LOCUS       DAOMTQ010000001        76056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_10_length_76056_cov_26.823805, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000001 DAOMTQ010000000
VERSION     DAOMTQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 76056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76056
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10_length_76056_cov_26.823805"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..607)
                     /locus_tag="PK518_00005"
     CDS             complement(23..607)
                     /locus_tag="PK518_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRM47319.1"
                     /translation="MMPTVYTVGHSNHSLADFLALLAQVDVQCLVDIRKLTGSRAQPQ
                     FNADALAAALATQGVGYLHLAALGGLRGRSPGMDGQRNGWWSNASFHRYADYACTQAF
                     AQGLQQLLMLSEQKRCALMCAEAVWWRCHRRIVADHLLAHGCQVLHIMGLGKVEPAHL
                     SAGAVVGEQGMVSYPGGDCNRVGLTPPGRAEGTP"
     gene            complement(604..816)
                     /locus_tag="PK518_00010"
     CDS             complement(604..816)
                     /locus_tag="PK518_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HRM47320.1"
                     /translation="MGQDFRIGEHVRWNSEAGFVTGRIIAVHTQDFDYKGHRHRATPE
                     NPQYEIQSDKTEHIAAHRGAVLQRIP"
     gene            1017..1364
                     /locus_tag="PK518_00015"
     CDS             1017..1364
                     /locus_tag="PK518_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRM47321.1"
                     /translation="MSDSVHPCLTCGACCHEYRVEFPVYELQSMGGSVPDALAHEVHG
                     NRWRMDGTQRHPVRCVALTGRCGKQSICSIYERRPSTCDQFQMGTERCNEARARHGLP
                     PLYAPREDFPLAA"
     gene            complement(1420..1689)
                     /locus_tag="PK518_00020"
     CDS             complement(1420..1689)
                     /locus_tag="PK518_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47322.1"
                     /translation="MLQELIVALIVVVAVAYMAWRYLPAAWRQRLGRVHPGLAAGPGG
                     CGSGGGGGCSSCGGCGTRAKAGVQQQSVAPPAPVARNADSLQSNQ"
     gene            complement(1693..3609)
                     /locus_tag="PK518_00025"
     CDS             complement(1693..3609)
                     /locus_tag="PK518_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRM47323.1"
                     /translation="MSAGQAAAAAFTHIALLGNPNCGKTALFNLLTGARQKVANYAGV
                     TVERKEGVFATASGRRVRVLDLPGAYSLHAQSLDEAVTRDVVTGRRPGEAVPELLVCV
                     TDATHLRLNLRLVLEARELGLPMVLVLNMSDMAQRQGIAIDRALLARELGMPVLATVG
                     VRTDGAQELLHWLDQARPDGPVQPRPPTDAPAPALNALEQDQPQRVQRLHQEVRRIMG
                     LAVREPAQRLEQDDRIDAVVLHPLWGTLLLAVTLFLMFQAVFSWAELPMGWIEGATAL
                     AAEWLNAHMADGPLRSLLADGVIAGVGGVVVFLPQILILFFFILALEESGYLPRAAFL
                     LDRIMGVVGLSGRSFIPLLSSFACAVPGIMATRSIPDWRDRLVTILIAPLMTCSARLP
                     VYALLIAAFIPEREVAGLFNLQGLVLFALYLGGILSAMAVAAVARLARRSAGVAPLLM
                     ELPSYRWPSPRNLALGLWERAGIFLRRVGGIILAVTVLLWFLSTYPAPPEGATGAAIE
                     YSLAGRLGAVLEMIFAPIGFNWQISIALVPGMAAREVAVSALGTVYAIAASADDTAAQ
                     LAPLLAGQWSLATALSLLVWFIFAPQCVSTIAAVRRETNGWRWPLVMTAYLFALAYGA
                     SFVTYRLALAWGGG"
     gene            complement(3606..3899)
                     /locus_tag="PK518_00030"
     CDS             complement(3606..3899)
                     /locus_tag="PK518_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRM47324.1"
                     /translation="MQLQGDTQATQQTQGLDSLPRGVPAVVAGLRQAEDGRASAMALR
                     LMEIGFLPGEPVRVIAKGFPTADPLAVRVGQATFALRRHEAAQVLVHVPRGAA"
     gene            4020..4343
                     /locus_tag="PK518_00035"
     CDS             4020..4343
                     /locus_tag="PK518_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRM47325.1"
                     /translation="MSTHPCLSCGACCASFRVDFSVHETEDMGGSVPQGLVVEVTGHT
                     ARMRGTDHARPRCAALTGQVGQRAACGIYEWRPSPCREFEAGSAACNRARQRHGLAPL
                     EGLVP"
     gene            4340..4981
                     /gene="kynB"
                     /locus_tag="PK518_00040"
     CDS             4340..4981
                     /gene="kynB"
                     /locus_tag="PK518_00040"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HRM47326.1"
                     /translation="MSYQPPLWDISAPLHASSPVYPGDTPYEQRWDARIGPDSAVNLS
                     TLTLSPHIGTHADAPLHYDNAGVAIGAVALQAFIGPCRVIHALGCGPLVEWRHIAHAV
                     SADLPPRVLLRTYASAPVDRWDAQLSAVAPETLERLADAGVLLVGIDTASVDPADSRE
                     LPSHQVLRRRGLRVLENLVLDAVPEGDYELIALPLKLTTADASPVRAVLRALA"
     gene            complement(5057..6106)
                     /locus_tag="PK518_00045"
     CDS             complement(5057..6106)
                     /locus_tag="PK518_00045"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRM47327.1"
                     /translation="MPTIPTIAPERLPALLAAMPKAELHIHIEGSLEPEMIFALARRN
                     GVDLPYANVEALRRAYAFTDLQSFLDIYYAGASVLLKEQDFYDMASAYLARAAMDNVV
                     HAEIFFDPQTHTARGVAIDTVIQGLHRACVDARAELGISASLILSFLRHLSEEDAFAT
                     LEQALPWMDKFIGVGLDSSELGHPPEKFARVFARCRELGLHVVAHAGEEGPPAYIWGA
                     LDVLKAERIDHGVQAVHDAALMRRLAAERIPLTVCPLSNLKLCVFPSLAAHNLPALLE
                     AGLVATVNSDDPAYFGGYINANFIQTFAALPILTARHAWQLAHNSFEASFAPQAEKRA
                     WGDRLQACFEHHASA"
     gene            complement(7051..8211)
                     /locus_tag="PK518_00050"
     CDS             complement(7051..8211)
                     /locus_tag="PK518_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM47328.1"
                     /translation="MTDLHKRSLFKLAALSAVAATALMGCGKKEEAAPAAPAPAPAAA
                     PAAKAEPLKIAFAYVGPVGDGGWSYAHDQARKKLEAEFGDKIQTSYVESVPEGPDAER
                     VLRDLVTQGNKLIFGTTFGYMDVMQKLAGEFPDVKFEHATGYKTSANMRIYDSRTYEG
                     AYLAGIIAGAMTKTNTLGVVGSVPIPEVLRNLNSFTLGAQSVNPKIKTKVVWVNEWFS
                     PPKETEAATSLINGGADVLFQNTDSPAVLKTAQEKGKRAFGWDSDMTAYGPKAHLGSA
                     VINWAPYYIKATRDVLDGSWATAQNWWGVKEGAIDLVSIAEDVPADIKAKVDAAKAGL
                     KDGSFVIWKGPIVDNTGKTVLDKDAAADDKFLTGINFYVQGVDGQVPGGDKK"
     gene            complement(8276..9433)
                     /locus_tag="PK518_00055"
     CDS             complement(8276..9433)
                     /locus_tag="PK518_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM47329.1"
                     /translation="MQHLNKRTLLKTAALSAIALAALAGCGKKEEAAAPAAAPAPAAA
                     APAADKLKIAFMYVSPIGDGGWTFQHDLGRKAIQEKFGDKIETSFVESVPESADAERV
                     MRDMAGQGNQLIFATSFGYQEFVQKAAADLPDVKFEHATGYKQAANVATYDTKTFEGA
                     YLAGIVAGGMSKTKTVGVVASVPIPEVVRNINSFVLGAQSVDPSIKAKVVWVNEWFSP
                     PKEAEAATSLINGGVDVMYQNTNSPAVLKTAEERGMRAFGKDGDMSAFAPKAHLGSAV
                     IDWTPYYTKVVTDTLGGQWQSGNFWWGVKEGAIDLLKIADDVPQDIKDRVAKARAGLK
                     DGSFAVWTGPVKDNTGKELLGAGQVADDKFLTGINFYVAGVEGKVPGTEGK"
     gene            complement(9458..10378)
                     /locus_tag="PK518_00060"
     CDS             complement(9458..10378)
                     /locus_tag="PK518_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM47330.1"
                     /translation="MESYALLLGSTLSAGTVLAIAALGLLINEKAGIVNLGAEGMMLC
                     AAIAAFATVVHTGSFALGLLAGMAAGALLAALFGWLVIWLNTNQYATGLALSLFGVGF
                     SAFVGISYVQAKLPDTPKFSWPVLGDLPVVGQALFRHHPLVYLTMVLVLLMVWFLYRT
                     RAGLVLRSVGESPGSAHALGYPVRRIRLLAVMAGGALCGLAGAYVSTVYTPLWVEGMV
                     AGRGWIALALTTFATWRPARVLLGAYLFGGVTMLQFHLQASGVQLASQLLSMLPYLAT
                     IVVLVLISRNPTWIRANMPASLGKAFHPGS"
     gene            complement(10378..11451)
                     /locus_tag="PK518_00065"
     CDS             complement(10378..11451)
                     /locus_tag="PK518_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM47331.1"
                     /translation="MLKLEARPQASRLWAYGSPLLALAITVLIGVALFAALGKDPLRG
                     LQVFFWEPIKSPYALGELALKATPLLLIALGLAVCFRSNVWNIGAEGQFVIGAVAAGG
                     VALLADKGTGPWIVPAILVAGVLGGMAWAGITALLRDRFNANEILVSLMLVYVATLLL
                     GYLVYGPWKDPMGYNFPQTKMFERVTQMPKLFAGSRVTIGLPLALIGVALLWAFLFRT
                     RAGFALQVGGLAPAAARYAGFSSRRALWTALLISGGTAGLAGALDVAGPIGQLTPFVP
                     AGYGFAAIIVAFVGRLHPVGMVFSALLMSMFYIGGELAQSRLGLPKSLTGVFQGLLLF
                     TLLACDTLIAYRVRWAAAQKGLA"
     gene            complement(11438..13039)
                     /locus_tag="PK518_00070"
     CDS             complement(11438..13039)
                     /locus_tag="PK518_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47332.1"
                     /translation="MNPPPQSDAAAMPTRAAPRLQLAGITKRYPAVLANDAISLTVQP
                     GEVHAVLGENGAGKSTLMKIIYGAVKPDAGQMLFDGRPVQVKNPQEARQLGIAMVFQH
                     FSLFDTLTVAENVWLGQDRRLPLAEVARRISATAAEYGLDIDPQRPVHTLSVGEMQRV
                     EIIRALLTAPRLLILDEPTSVLTPQAVDKLFVVLRKLASEGCSILYISHKLHEIRELC
                     SACTVMRAGKVTGVCNPAQESNASLSRLMIGAEPPRLTHRALSAGEVVLHTRNLSLPA
                     TDPFGVPLLAIDLDVRAGEVVGIAGISGNGQKELLYALSGEDQRAQPAMVQLAGKNAG
                     RLSPRRRRALGLHFVPEERLGRGAVPSMSLAHNLLLTRGNAVGRGGWLRMGQLQHQAR
                     AIIERFHVKAGGPNAPAQSLSGGNLQKFIVGREIDAAPRLLIVSQPTWGVDVGAAAQI
                     RGEILQLRDKGCAVLVISEELDELFEICDRLHVLAKGRLSPSVPRAEATVARIGEWMS
                     GLWHADVQQHLGAGGADQGGGHAQA"
     gene            complement(13179..15272)
                     /locus_tag="PK518_00075"
     CDS             complement(13179..15272)
                     /locus_tag="PK518_00075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM47333.1"
                     /translation="MNLPAHQPAAWEPEAEESTASGWFPGPLVRKLLLVALAAIALSG
                     SLAAWWVSHAMGQEAMDRLVSQQNDEVELVARLLASKIEQSQKVLSTVAEGITPEILE
                     SPASLGWLLQQGLPAVRFFDAIQVARKDGTLSVNLRYGRLAKASDLDPTERDYLVRTL
                     VNGKPLVSGLIGNSPTDARVMFTMPLLRGEGRVMGVVSGVLRLQSQGLLPHSLSLPER
                     SDSRLVIFTRDGVILSHPNLERVLGQVSDEPGLAQAYADWRAAGQTFAGGAATQPQAD
                     HVVSLAGVPMPQWMVARVSDAQSMLAPLQQAQRRAWGQVAVAAGGVCLLATLFMLWLA
                     RPLTLLRLRVRAALEADERPASTAEGADEPWPRSSGEVDDVVRVCTRLLEGRRKSQRG
                     ADTLVRQLQAVLEHVPLGIAVTRAEQVEVVSQQVCRLLGYAPQELQGRNLIDLLAPHE
                     DGLDTAQQVHEAFAAHGAFDGELPLLRKDGSVLWGHVRGQLVQPGEPGRGVVWTVQDC
                     TAEREARAQQAWESSHDALTQLLDHGEFERRLRVLLAERAQRAAQPGALAPEQGVLLF
                     MDVDHFTVVNDLAGRAVADDVLRRLARLLEAEVQRSGWAARLGGDEFAVLLPASSLAN
                     GQAVAERLRATVRAWEPAYQGRSFTLGLSIGLVPLAPGLRDAAAVLHAADMACYEAQC
                     AGRNRVHLGAVRVRA"
     gene            complement(15525..16148)
                     /locus_tag="PK518_00080"
     CDS             complement(15525..16148)
                     /locus_tag="PK518_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM47334.1"
                     /translation="MFPIPSSDQSVQIEVATAGEVLRQRHQQPASVLYLDSGRVLLGV
                     REDGVLRHQLGVIEGPFWLDAATALLDQPCAVDMVADTRVQLRRVPLEDFKRSFDDLP
                     EPAQALLRDMATGYCQQTELAVSRLAQDAEARCAQWLLRHAHNAEDGGLRVTLHQRKR
                     LIAAQLGIAPETLSRVLRHLREHGLITGTGNVLNLPQPRALQMVAGF"
     gene            16420..17445
                     /locus_tag="PK518_00085"
     CDS             16420..17445
                     /locus_tag="PK518_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRM47335.1"
                     /translation="MNHKLIALATAALCAAGAHAQSSVQVTGLMDVYAGSMKMAGDAK
                     RKSVVDSGGMTTSWFGFKGTEDLGGGLKASFQLTSFIQVDDGTQGRFPKDTFFSRDAN
                     VSLSGGFGSVLLGRWMAPNFLPSVIGNPLGDSFEFSPLILHKNVPLLNASNWKATTPS
                     DTGWSNQIVYSTPNIGGFKANLQYQFGEQPGDNGKKNIGANFFYFGGPLTVTGYYERD
                     QISNPAVPNTYLGTTKKDWMLAAAYDFKAVKPYLSYGQTKADNSPDKAKTLQLGASAP
                     LGGGSLLASWAKTELSASNLDRKTFTVGYDYYLSKRTDVYAMFMNDRITSQPTGNSFG
                     VGIRHRF"
     gene            17538..17975
                     /locus_tag="PK518_00090"
     CDS             17538..17975
                     /locus_tag="PK518_00090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRM47336.1"
                     /translation="MPMFVDTSPPGQCIFCRLVQGSIPAAKVYEDALTIAFMDIGQVT
                     PGHVLVATRRHAATLLDATPEEAAAVMQTAHRVARAIQASFDPPGLTLLQANGILGWQ
                     TVAHFHMHVVPRREGDGLGLTWPRQEPPAQTLAGYAERLRAAL"
     gene            18177..19421
                     /locus_tag="PK518_00095"
     CDS             18177..19421
                     /locus_tag="PK518_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRM47337.1"
                     /translation="MSVPSAAIGAQQAPAVPAGLAIVVLALLLSIQPVTTDLYLPALP
                     ALTRGLGADVAAGQLTLSALLLAFGCSQLAWGPLSDRFGRRPVLLAGLALYTLASVGA
                     VLAPSMDLLVAWRTAQGLAMGAVVMCARAIVRDLYTPLAGARAMSKALTGLGLIACLC
                     APLGGLLTEWLGWRAALLVLTAYAVATLALVALRLPETLGQRNPRALHPATLVRTWAL
                     VLRTPTFWAFSLLTTASYGGLFTFLAASSFVYLDVLGSSRTQYGLTLLSTAAAYLLGT
                     LLCRRLLARHGLKRTVAIAGLLSASGGLLMLLAAALGWHSVAALLPPFYLFMLGHGIH
                     QPCGQSGAVGPFPQAAGVASALNGFMMMLAAFAIGRWLGGALDGTVWPLVQGVALWSL
                     LLAAIAWTLVQRHAPQESADAP"
     gene            19411..20382
                     /gene="miaA"
                     /locus_tag="PK518_00100"
     CDS             19411..20382
                     /gene="miaA"
                     /locus_tag="PK518_00100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRM47338.1"
                     /translation="MHRDPIPAIALAGPTASGKTAAAFAIAEALAPRLPVEIISVDSA
                     LVYCGMDIGTAKPTRDELARVPHHLIDIRDPLQAYSAAEFVRDATRLITEIRARGALP
                     LLVGGTMLYFKALLDGLDDMPAADPAVRARLEQQAAAIGWAGMHAELARVDPQTAARL
                     APGDSQRIQRALEVWHVSGRPLSSFHTIKTEAYNARPISDTSLFSLEPENRTWLHERI
                     AQRFHAMLAAGFLDEVLRLRARGDLHPDLPSMRCVGYRQAWESLDGLHPMASLPERGI
                     AATRQLAKRQITWLRSMPGRHTIACDAPDAGAQLVRAVCAHVLGTGA"
     gene            20379..21038
                     /locus_tag="PK518_00105"
     CDS             20379..21038
                     /locus_tag="PK518_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47339.1"
                     /translation="MTGAAPLLQVRGLARRYGAQSVFENVHLEVHAGEFVAIVGDSGV
                     GKSTLLNCLAGLDRWDAGQVLHQGVDLGRLDDTQRALWRRSHVGFVFQAFHVLPHLDV
                     AQNVGLPLLLQGRPDAARVGQMLAAVGLDGLGDRLPHQLSGGQLQRVAIARALVHRPA
                     LLLADEPTGNLDPGTAARIMDLLLAQTREHGAALVLVTHSAAAAARAARVLHLTAQGI
                     R"
     gene            21150..22529
                     /gene="soxC"
                     /locus_tag="PK518_00110"
     CDS             21150..22529
                     /gene="soxC"
                     /locus_tag="PK518_00110"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HRM47340.1"
                     /translation="MAQIQAQPAPASQERGRLRKAPEHFLDATGVRTVFAEGRQGRRN
                     FIRSAFAAAMAGSAASHAAAQARDTGDGDPAILNLPAHSTGLGQPVATDGYGRPSQYE
                     GNVQRRQSPGLTQTNQASVSFAPLQSLFGIVTPSGLHFERHHQGWWDVDPAQHRLMIN
                     GMVKTPKVFTMDEIMRLPSVSRFHFIECGANTAVDWGNVAVPTCQYTHGMLSCSEFTG
                     VSLITLLEHAGADLKAAKFVLAEGADGSSMTRTIPMSLVTSGQVLVAYGQNGEMLRPE
                     NGYPLRLVVPGVQGVSWVKYLRRIELGDQPWASKDEAIHYIDLMPDGQHRQYTSIQEC
                     KSVITTPSGGQVLLDKGFYNITGLAWSGRGKVKRVDVSVDGGRNWRTARLETPVLDKC
                     LTRFNLDWVWDGKPTIVQSRAMDETGYVQPSYKQLREVRGTRSIYHNNAIQSWLVQEN
                     GEVKNVQIA"
     gene            22513..23586
                     /locus_tag="PK518_00115"
     CDS             22513..23586
                     /locus_tag="PK518_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM47341.1"
                     /translation="MFRLRDTLAAALLAAGVALPALAQDKFPGIGRAATPKEVAAWDI
                     DVRPDFKGLPPGKGTVAQGQDIWEERCASCHGFFGESNSVFMPLVGGTTADDVKTGRV
                     ATLRRTDYPGRTTLMKVPTVSTLWDYIHRAMPWDKPKSLKPDEVYAVTAFLLNLAHVV
                     PDSFTLSNDNIAEVQQKMPNRNGMTLAHALWPGKDMPGLATQPDIQAKACMSNCGPEP
                     VVTSVLPEHARNNHGNLREQNRTVGAQRGSDTTRPEGWLGDAAGPVKVVGPASHNSPN
                     AAAIALTHKHACTACHNMEGKLVGPSFADIAKKHGGKADYLAEKIKSGGSGLWGEIPM
                     PPQTLPQDDTGAIAAWLAGGAGK"
     gene            23663..24124
                     /gene="soxY"
                     /locus_tag="PK518_00120"
     CDS             23663..24124
                     /gene="soxY"
                     /locus_tag="PK518_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HRM47342.1"
                     /translation="MQTTRRQVLKHSAVVAGLLAGTGLFPQYALAFNKAAFDAKSLAD
                     ALKALGANGAPEASSAVTLTAPDIAENGAVVPLAVASTLPGIKQLVLLVEKNPNALVA
                     VFNMGPELEASFATRAKMGESSDVYAVAITGDGKAHFAKKEVKVTLGGCGG"
     gene            24178..24489
                     /gene="soxZ"
                     /locus_tag="PK518_00125"
     CDS             24178..24489
                     /gene="soxZ"
                     /locus_tag="PK518_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HRM47343.1"
                     /translation="MADPMRIRAQAQGDKATVRVLMSHEMESGQRKDASGNLVPAWHI
                     QDVSASLNGKQVFSCEWGPGVSKNPFLQFVVKGAKAGDKLSVTWKDNKGESRTDEATV
                     S"
     gene            24568..25377
                     /gene="soxA"
                     /locus_tag="PK518_00130"
     CDS             24568..25377
                     /gene="soxA"
                     /locus_tag="PK518_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HRM47344.1"
                     /translation="MRGKSIALITAALGLMGLSGLALAQKTSIEAIEEYREMLQDGNP
                     ADLFEAKGEDLWKKKRGPKNASLEGCDLGLGPGVVKGAFVELPRWFADTGKVQDLESR
                     LLTCMSALQGIDPKVIIDGQWGKGERANTTALATWIAAQSKGMAFHLPQGHAQERTMY
                     EVGKRLFYQRGGAHDFSCASCHGEAGKRIRLQDLPDLRKAPGDGIGFAAWPAYRVSNG
                     QMWSMQHRLHDCYRQQRFPEVVFASDVTVALGVFLGVNATGDKSAVPTIKR"
     gene            25395..26069
                     /gene="soxX"
                     /locus_tag="PK518_00135"
     CDS             25395..26069
                     /gene="soxX"
                     /locus_tag="PK518_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HRM47345.1"
                     /translation="MKPQITTACLGLLAAAALAGCAGGSRTAGLPSPAELDALTQKAV
                     QESFKDHGIVKVKNVFADDETISACNAADAAGKPLDDATAKRLEALNMKTVQWPSDGK
                     FLGSWKEGEKLAQSGRGLTFTDTTKTPNGGNCYNCHQIDKKEISFGTIGPSLYHYGKL
                     RGVKDPNAPEARAMVEYTWGKIWNSKAYSACSNMPRAGHMGIMTKSQVADLVALLLDP
                     QSPVNQ"
     gene            26158..27891
                     /gene="soxB"
                     /locus_tag="PK518_00140"
     CDS             26158..27891
                     /gene="soxB"
                     /locus_tag="PK518_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HRM47346.1"
                     /translation="MNLTKREFLQVLTAASAAGMGLGRYAHASTDSAAQGLYDIPKFG
                     NVSFLHITDCHAQLKPIYFREPSVNLGLGDMQGRLPHLVGEQLLKAAGMKNGTAAAHA
                     LTYLDFEQAAQRYGKVGGFAHLATLVKRMRASRPGALLLDGGDTWQGSGTALWTHGQD
                     MVDACKLLGVDAMTGHFEFTLGMQRVQEIVEKDFAGKVDFVAQNIKTTDFGDPVFKPY
                     VIRQINGVPCAIVGQAFPYTPIANPRWMVADWEFGIQDENMQKVVDEARAKGAQVVVV
                     ISHNGMDVDLKMASRVRGIDAILGGHTHDGMPVASIVKNAGGQTIVTNAGSNGKFLGV
                     LDFDVKGGKVADFRYKLLPVFANMLPPDKDMDALITKIRAPYEGKLAEQLAITEGLLY
                     RRGNFNGTGDQLIVDALMEVQGADLAFSPGFRWGTTLLPGQPITREWLLDMTATTYSF
                     ATLTEMKGEMIKTVMEDVADNLFNPDPYYQQGGDMVRVGGLTYAFDPLQGMGKRVSDM
                     RLKGELIDPNKTYKVAGWAPVAEDAAKAGNPMVWELVEQWLKARGGKVTARQPNVPRL
                     TAGLPNPGYAD"
     gene            complement(27902..29098)
                     /locus_tag="PK518_00145"
     CDS             complement(27902..29098)
                     /locus_tag="PK518_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRM47347.1"
                     /translation="MQKQVLIAGGGIGGLAAALGSARAGWEVRLFERAGAFTEVGAGV
                     QIGPNVVRRLQAWGLHRALLAVASFPERLRVRSALTGAELGALRLGATAVERYGAAYA
                     TIHRADLHGLLLDVARKSSLVHLNLDHQVERHEEQGDAVTVRLVRGDGKVIEVEGDAL
                     IGADGLRSGTRTQLLGEAATRVSGHLAYRAVLRQDALPQALRSNDVTVWLGPNLHVVQ
                     YPLRRGELLNLVAIRHGQPPADLDSWDHRVNAADLESALAQTCTPLQDLVRVVPQVGE
                     GWRLWPLLDRPPVAGPQEMARGLVALLGDAAHPMRPYLAQGAGMAIEDAVELEKSLAM
                     HDVDVALCLRRYALNRWQRNARVQERSRRNGQIFHASGPLRWGRDAAMGVLGERLLDQ
                     PWLYRG"
     gene            complement(29130..32045)
                     /locus_tag="PK518_00150"
     CDS             complement(29130..32045)
                     /locus_tag="PK518_00150"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRM47348.1"
                     /translation="MAVTTTPSHAFASTVKNFTTASGKKGKFYSLPELAHKYPRVSRL
                     PVSIRIVLESVLRNCDGRKVTAEHVRQLASWGANAARKDEIPFVLSRVVLQDFTGVPL
                     LVDLAAMRSVTQRLGKDPKKVEPLVPVDLVVDHSIMVDYYGSKNALQLNMKLEFQRNR
                     ERYEFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKSADGVYYPDTLVGTDSHT
                     TMINGIGVVGWGVGGIEAEAAMLGQPLYFLTPDVVGFELTGQLREGVTATDLVLTVTE
                     QLRRAKVVGKFVEFFGEGTRSLSLPDRATIGNMAPEYGATMGFFPVDEATLDYFEGTG
                     RTQDEIETLEAYLKAQGLFGIPRAGDIDYSQVVRLNLADVTPSLAGPKRPQDRIDLGQ
                     VKQQFTSLFSKPVEQAGFNLAPERLHERHAVAAHAPDAAQAVPDERPATEGAPRFEAE
                     MVHNKPRLEVQHVDAPMHGHQGDRLTVGSGDVLIAAITSCTNTSNPSVLLAAGLLARK
                     AVEAGLKVQPHIKTSLAPGSRIVTEYLTETGLLPYLEKLGFTVAGYGCTTCIGNAGDL
                     TPQLNEAITQHNLVCAAVLSGNRNFEARIHPNIKANFLASPPLVVAYAIAGTMLKDLM
                     TEPVGKGKGGKDVYLGDIWPKAEEVQALLKFAMNGKAFRENYAKVKTEPGALWDKIHG
                     VSGEVYSWPASTYIAEPPFFADFALDTVAVGEQGEGAGGQKHSESVTGMRIMALFGDS
                     ITTDHISPAGSIKESSPAGLWLKAHGVAKADFNSYGSRRGHHDVMMRGTFANVRIKNL
                     MIPALADGSREEGGMTLYQGDGPQRGSKMAIFDAAMQYMASKTPTVIFAGEEYGTGSS
                     RDWAAKGTQLLGIKAVVAKSFERIHRSNLVGMGVLPLQLRAGDSWESLGLKGDEIIDV
                     LPDAALTPQSQALLVIRRADGSRQEVAVTLRIDTPIEVDYYRAGGILPYVLRQLLSS"
     gene            complement(32345..32641)
                     /locus_tag="PK518_00155"
     CDS             complement(32345..32641)
                     /locus_tag="PK518_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HRM47349.1"
                     /translation="MNPSYKDLLKQREALELQISEARDRELAAAVAQVRGLIAEFGLT
                     QQEVFPVGRGARTSASIGVKVAPKYRDPATGQTWTGRGKAPKWIQNEDREKFAI"
     gene            complement(32700..32906)
                     /locus_tag="PK518_00160"
     CDS             complement(32700..32906)
                     /locus_tag="PK518_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47350.1"
                     /translation="MLIVDGGFEWGDCPAPDRQALRTGESGVVDKCFLTIEVGEYSFN
                     ERTGVMTCRVHQHSSVIHADKLVT"
     gene            32907..33752
                     /locus_tag="PK518_00165"
     CDS             32907..33752
                     /locus_tag="PK518_00165"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HRM47351.1"
                     /translation="MALYCAGDIQGCGDAFERLLSVIGFSPSRDTIYLLGDLVNRGPA
                     SAAVLRRCMQMGDSVRALLGNHDLHLLATAHGVRPPSRRDTLDGILQASDRGAMLDWL
                     RQQPLARQVEHGGEPLLLVHAGVLPQWSADDVLALAREVQAQLSGPGLAEFLRRMYGN
                     HPDLWDAGLQGADRLRIIVNALTRLRFCTPEGRMDFDSTESAAQAPEGLVPWFDAPGR
                     RTRGTLIAFGHWSTLGWISRPDILGLDTGCVWGGALSAVRFGATLAEREHCKVQCPQA
                     QRPGE"
     gene            complement(33853..34893)
                     /locus_tag="PK518_00170"
     CDS             complement(33853..34893)
                     /locus_tag="PK518_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRM47352.1"
                     /translation="MNNGWQGGQLSRRRFALALPLLGAVAWASPGLAQAAGPQRASRN
                     LMGTRVDIVADGGDARYLQAAIGLAFDEMQRLEALLSRYREDSVVRRIGHAAGRHPVA
                     VSPEVLAILRNAQQVWRESAGAFDPTVGALSGWHFEAGQQAMPAATDIAAALRHVDAR
                     KLRLDERAGTAYLSERGMQLDLGGIAKLPILAAGLRVLEREGVANALVNGGGDVLASG
                     RLQGRPWRVGVRDPRAPEKLLGAIEVEGFGIVASSGDYERGFMHQGRRLHHVLDPQSG
                     WPTSGVHGVALLARDVRAVNGWGTALMVQGMAAVPAWSAGHPDVAVLAAGSDGALWQS
                     RGMAAALKSIAA"
     gene            complement(34911..35327)
                     /locus_tag="PK518_00175"
     CDS             complement(34911..35327)
                     /locus_tag="PK518_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM47353.1"
                     /translation="MKKTSLTMALAAATLLLAACGKNEAPAPAPAPAPAAEAPAAAPA
                     PAPAPAPVAENTAGKSVYGKTCAMCHAAGVAGAPKPGDKEDWGPRIAQGKDTLYKHAI
                     EGFTGAKGMMPARGGASSLSDDDVKAAVDFMADQSM"
     gene            35552..35923
                     /locus_tag="PK518_00180"
     CDS             35552..35923
                     /locus_tag="PK518_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47354.1"
                     /translation="MDFLRLLNRQRRWIVAWFFLSLGAALAAPMVQARTMELVCTGGN
                     GLSLVVHSSDGKAAPDTLGMDCPLCLLGASAPPPVRPLPQAAPLPHYTFAPPSVAHAL
                     PPMAAPPPARAPPVFPSVYPS"
     gene            35936..37870
                     /gene="nosZ"
                     /locus_tag="PK518_00185"
     CDS             35936..37870
                     /gene="nosZ"
                     /locus_tag="PK518_00185"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HRM47355.1"
                     /translation="MSDKKDSLSTETAGIGRRRFINTAALAGLTVGVAACNDKPAAAP
                     AASAPAPAPAPAAASGAAASTHLKPGELDTYYGLWSGGHTGDMRVLGMPSGRELHRIP
                     CFVPDALVGWGVTNESKAVMGTKPDGHLKYTVADTHHTHASYKDGNYDGRYAWINDKI
                     NSRIARIRLDYFVCDKITQLPNVQGFHGIFPDKRDPVDPAINYTTRVFCGGEFGIPLP
                     NNTGIEDGSKYRSLFSCVDAETMEVRWQVLIDGNCDLVATSFDGKLAATNQYNTESGV
                     HYEDMMSAERDACLFFNVARIEEAVKAGKFKTFGTSKVPVVDGTRDSNKDPLTALTAY
                     VSVPKNPHGVNASPDQKYFICAGKLSPTTTTIELVKVLDWFDGKMEKLDDAIVAEVEV
                     GLGPLHTAFDGRGNAYTTLFLDSQIVKWNIEKAIAFHKGDKNAKYVVDRIDVHYQPGH
                     INASQSETVAADGKYLAVGCKFSKDRFLPVGPLHAENEQLIDISGEKMVLLADHPVRG
                     EPHDFIIFKRDLLKPKQVYDLDESPLAIKDPKESGVTRNGKKVTVKITSQAPAFSLRE
                     FKVKKGDEVTLILTNLDKIEDLTHGFAIPNYNVNFIVNPLETKSVTFIADQPGVFWCY
                     CTHFCHALHLEMRTRMIVEA"
     gene            37934..40555
                     /locus_tag="PK518_00190"
     CDS             37934..40555
                     /locus_tag="PK518_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM47356.1"
                     /translation="MSAFGQRFAFFWAALLLVLALAAPRAHAGAYEAELPANLATTPD
                     MCSLLPCGEVFPGAKHFSERKGQPPYVEAYDSDGADKKLLGYVMLSTDITDTPAYSGK
                     PVVTLIGMDKEGHFVGIQVLKHSEPILLLGIPESALINFNNQYLGKSVTDTIEVGPSR
                     PDENVIGVDAISGATVTVVAQNQVVMASGAAVARQTGIIAPTVREPARFIAGGQNYDW
                     AQLVQMGVVKQLLVKPEQVGLPRGPEPFIELWYGDLNHPDVGTSLLGKHNYERLHASL
                     KEGENALFVIKTAGQESFKGSGFVRGGIYDRVQVKQGADSFTFRDLDSLNLYGLEAQG
                     APRYTESAIFIIRSHSFSAAYPWKFAFLGNRVDRATGQRSFAVFESKYWLPAALLEGG
                     RPKVEEPAAPWVRIWKSQALPISLFALLLVAVTVVYALREKLTRLSTHKNKWPVNGFK
                     YTFWAISIFWIGFGYMAQPSITQVLTWFHSLLFQWTWSLFLSDPFIFLFWIFIIVTVF
                     LFGRGLFCGWMCPFGSLQEGIYKIAKAVGLKRFQTALPQKWHDRLKWVKYAVFFGLLA
                     VSMFSMGLAEKLAEVEPFKTTFLVGVMNRAWPYGLFVAAILGVSIFIERPYCKYICPL
                     GASLAMPSTFRWFGLKRKQDCNSCKACAVGCGAQAIDADGRIDHRECLHCLDCMILYT
                     DTKGCPPLAMERKRRERDGLEITPIGKNGYFIPIHPAKVEDQISPKAKKGVDPRMPTD
                     RTLPAHKEDVGLLEWLWLELRDHLWPWSREGWHSQKTLQIAGLALAIAATIAWVQAAL
                     GHLSSGAIIGWWFGWSVYEVLIRLSGRRYVKDGPWWRDQYRVAGVMDMMSYVGFKNLM
                     IGAALFLVLKTLGWLIA"
     gene            40552..41820
                     /locus_tag="PK518_00195"
     CDS             40552..41820
                     /locus_tag="PK518_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRM47357.1"
                     /translation="MTMLPHLAAALALAIAGACQAATISVRPGDDLAEAVTKAQAGDV
                     IEVARGQYRANLLIDKPLTLRGLDRPTISGGNQGDTIRVTAPDVLIEGLIVRDSGDSL
                     KDQNAGIYLYPGAHRAIVRRCDLTYNLFGLWIEKANDVLIEHNTITGKREYASPQRGN
                     GVQLYNTKGAKILNNDISFVRDALYVDVSHNALFKGNKLHHSRYGTHYMNSYYNLWED
                     NDTFYNRGGLALMEVRHQEVRNNRAWGNSDHGIMLRTLQDSDISGNVVAGNNRGFFIY
                     DVEYIKLNDNLVVDNVVGVHLSAGSTRNEVEGNDFIANREQVRYVGARDEVWGKQRGN
                     YWSNYLGWDRNNDGKGDVQYEANDMVDRLTWRHPSIKLLLASPAVQALRLVGQQFPVL
                     RVPSVVDPHPRMRPHHQDWSQWRGKHFPGQ"
     gene            41831..42733
                     /locus_tag="PK518_00200"
     CDS             41831..42733
                     /locus_tag="PK518_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47358.1"
                     /translation="MAISLRGVRKHFGSLHAVDGVDLDIPQGEIFGLIGHNGAGKSTL
                     FKMMLGLLAPTSGSILVAGTPVGGRNFRAARRQLGYLPENVVLYDNLDGLETLHFFAR
                     LKGAPLAECPAMLEKVGLAHAGKRPVREYSKGMRQRLGFAQALLGNPRVLFLDEPTNG
                     LDPQAIRDFYATLRDLQQQGVTVIITSHILAELQERVDRLAILVSGKVEALGSVHELR
                     ERTGLPIIVEVDLAPQDCHDALHVLHQVTGVKPAQTPSGLQLQCPREHKMAALAALTP
                     LGARVQDLRLHEPSLEDMFFGISA"
     gene            42759..43574
                     /locus_tag="PK518_00205"
     CDS             42759..43574
                     /locus_tag="PK518_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM47359.1"
                     /translation="MELTPILTVAAKEFRDRMRNRWVLAVALVFTVFSLVIAYFGGAQ
                     QGQVGFRSIEFTIASLVSLVIYLIPLIALLLGFDAIVGERERGSLDLLLALPITRLEL
                     LLGKYLGLALALSLSTVVGFGLVAVLLWRHMNANALFHYGGFMLSSVLLGLAFLSLAV
                     LISVLARERTRASGLAIATWFFFVLVFDLLLLGLLVTTGGQFAGDSFAWLLLLNPADV
                     FRILNVFSLEDVRTLYGLASVVPQSLGNPLTMGGVMAAWIALPLALANWRFKP"
     gene            43571..44119
                     /locus_tag="PK518_00210"
     CDS             43571..44119
                     /locus_tag="PK518_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRM47360.1"
                     /translation="MTCSCITRRRALGFAALAALSTAGLLSGCGDKAAEQTSLAPVEI
                     DRSTSCELDGMLLADYPGPKAQIHYAGQDKPSFFCDTVELFNTLLAGEQVRPIRAVYV
                     QDMGQAQWEEPKGHWIDAKSAIYVLGSKRHGSMGPTIASFAQEADATKFAGEHGGKVL
                     RFGDIKLDMVDLSGGALHDTRM"
     gene            44249..44794
                     /locus_tag="PK518_00215"
     CDS             44249..44794
                     /locus_tag="PK518_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRM47361.1"
                     /translation="MLSPEAPASLEPDVCIVAPPTPYDPASGLPPTAARAVPPDVRCP
                     VCGMFPARSPQWAAQIIFSNGDAQYFDSPLSLFMYLQDVARYSPGRSSGQIAAHYVTD
                     ASAPPDGKRTPWVDALSAYYVHGSSAKGPMRAGNLPAFATRAAADAFAERRGGVVLAF
                     GAIDAALVAELASGGGHSHHQ"
     gene            complement(44887..46335)
                     /locus_tag="PK518_00220"
     CDS             complement(44887..46335)
                     /locus_tag="PK518_00220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM47362.1"
                     /translation="MTQAYSSLALAEPLKRAVADMGYENMTPIQAQAIPVVLTGKDVM
                     GAAQTGTGKTAAFSLPLLERLLKHENSSASPARHPVRALVLLPTRELADQVAQQIALY
                     AKYTKLRSTVVFGGMDMKPQTIELKKGVEVLVATPGRLLDHIEAKNAVLNQVEYVVLD
                     EADRMLDIGFLPDLQRILSYLPKSRTTLLFSATFSPEIKRLAGSYLQDPVTIEVARPN
                     ETASTVEQRFFSAQDDDKRRAIRHVLTERGLKQAFVFVNSKLGCARLARSLERDGLKT
                     AALHGDKSQDERLKALEAFKSGEVDLLVCTDVAARGLDIKDVPAVFNYDVPFNAEDYV
                     HRIGRTGRAGASGLAVTLVASSDTRLVTDIEKLIKKKIELEPLELAEDRPRGRFNDGR
                     RAWREDGEDNRGAPRGGRERSSYRSAPAVVRDPFFDKPYEAATADSLPPSWEVVPKAA
                     PARGSISANIKPRRKLAALFKAPVPEAEPTAQ"
     gene            complement(46424..47290)
                     /locus_tag="PK518_00225"
     CDS             complement(46424..47290)
                     /locus_tag="PK518_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRM47363.1"
                     /translation="MKWEGNRESDNVEDRRSSGGGGGFGGGRSIGIGTVVLALIGWGV
                     FGINPLTTIGVLSGADAPQVQQAPAQRPPGDDRQAAFVSTVLASTEDVWGQIFQQGGA
                     QYRQPKLVLFRGATPTACGTGQSAMGPFYCPGDQKVYLDMDFFDTMSRQLGAPGEFAR
                     AYVIAHEVGHHVQTLLGATAKVDGMRGRISQRDQNALSVRLELQADCYAGIWARHSQQ
                     AKNWLDQSDIESAINAAQQIGDDTLQRQQTGTVRPDAFTHGSSAQRVRWFSQGLKTGS
                     VQACDTFKAQAL"
     gene            complement(47435..48001)
                     /gene="dcd"
                     /locus_tag="PK518_00230"
     CDS             complement(47435..48001)
                     /gene="dcd"
                     /locus_tag="PK518_00230"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRM47364.1"
                     /translation="MSIKSDKWIRRMAEQHGMIEPFEPGQIREQDGKKIISYGTSSYG
                     YDIRCAREFKVFTNIHSTVVDPKNFDEKSFVDFEGDYCIIPPNSFALARTMEYFRIPR
                     DVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFF
                     QGDEECAVSYKDRKGKYQGQHGVTLPKA"
     gene            complement(48114..49331)
                     /locus_tag="PK518_00235"
     CDS             complement(48114..49331)
                     /locus_tag="PK518_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRM47365.1"
                     /translation="MESYLLDWANLLLRWLHVITAIAWIGSSFYFVFLDSSLTPPEDE
                     GMKRDGVSGELWAVHGGGFYHPVKFNVSPPKMPDHLHWFYWESYTTWLSGFALLTVSY
                     LWNAGIYLVSPSNPLMSSSMAIVAALGFLVVFWLLYDAICRAFGQKPNGDATVGAMVL
                     VLVCIAAYLACHIFPGQAAFLLMGAMLATSMSANVFFWIIPGQRKVVAALKAGQPVDP
                     MHGKRAKQRSVHNTYFTLPVLFAMLSNHYGWLYNHELNWLVLILMMFAGAAIRQFFVM
                     RHGFKLGRNTHPWPYATVGVLVLLGVLGWLKPAPQAAAAMASGAATQPAGYGQVQAVM
                     QKHCVVCHSANTVAQKNVKFDTPDEVKGHAQQIYQQVVQLRKMPFGNPGALSVEEVDT
                     FKRWYEGGATVTP"
     gene            complement(49537..50364)
                     /gene="xdhC"
                     /locus_tag="PK518_00240"
     CDS             complement(49537..50364)
                     /gene="xdhC"
                     /locus_tag="PK518_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HRM47366.1"
                     /translation="MAALNSLLERIKTTPACLVTVESTQGSVPREVGAWMALFMGQTP
                     ALGTIGGGHLELQAMQEARERLAEHLAGLPARPPLLRQALGPSLGQCCGGVVHLRFEC
                     VDAADAPALRQRLAPRLQPVALFGGGHVGHALARVLAPLPFALHWIDSRDEVFPAQVD
                     ADTLCEHSDPVQAAVPELAPQSMVLIMSFSHAEDLDIVAACLARQRERADLPFIGLIG
                     SRTKWATFSHRLLARGFSDQELAQVTCPIGVSGITGKEPEVIAVAVAAQLLQRLGTN"
     gene            50490..50942
                     /locus_tag="PK518_00245"
     CDS             50490..50942
                     /locus_tag="PK518_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47367.1"
                     /translation="MAAVAAIVAACKSGPQPPPAQDAPPPQPAGTAAGPVRSSAAATP
                     RAYRRDAASHLYGLNPQRIYKGKLPPLLYAIGVLEVDIDRMGRVSALRWMRAPRHAPE
                     VIREIERTVRAAAPFPVPARMQRVTYTDTWLWDKSGHFQLDTLTEGQL"
     gene            complement(50947..51297)
                     /gene="uraH"
                     /locus_tag="PK518_00250"
     CDS             complement(50947..51297)
                     /gene="uraH"
                     /locus_tag="PK518_00250"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRM47368.1"
                     /translation="MGLSTHVLDTMHGCPAAGMAVALYATQGDQATLLKSLVLNHDGR
                     TDAPLFDNHSLKAGTYRLTFDVAGYFKARGVQLPEPNFLNQVSLDFGVAHTDQHYHVP
                     LLVSPWSYSTYRGS"
     gene            51426..53204
                     /gene="ggt"
                     /locus_tag="PK518_00255"
     CDS             51426..53204
                     /gene="ggt"
                     /locus_tag="PK518_00255"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRM47369.1"
                     /translation="MQLRPTLLAASLALALFGLAGCGSTPQQPSAAATAQPGAAQAAA
                     AAYDFDMDIFHPVVASNGMVASEQELASRIGLDILKSGGNAVDAAVAMGFALAVALPN
                     AGNIGGGGFMMVHDAKTGKSVALDFREVAPAKATRDMYLDAQGKVIDGKSLYTHYAVG
                     VPGTVAGMEHALKRWGSLPLSRVVAPAVALADKGFPVSETLAKILQQEQKNMGRWPAT
                     QAIFWKNGAPLKRGDALVQKDLAQSLRLIGEQGAKAFYEGAIAQKIAAEMAPHAGALS
                     LQDLKNYKVVEREPVRGSYRGYQIVTMPPPSSGGAHLIQILNMMERWPMNQWGVNSAK
                     SMHHMAEGMKLAYADRAEYLGDPDFVKVPLKGLISKRYADQLAAGINATQARSAKDIK
                     PGKPQPYESDQTTHYSVVDKAGNAVAVTYTLNTNFGSGIVAKGTGIMLNNEMDDFSAK
                     PGVANAYGLVGGEANAVAAGKRPLSSMTPTLVLKDGKPTLVTGSPGGARIITTVLQTV
                     VNTIDFGMNPAEAAATPRFHHQWTPDELRIEKGFSNDTLALLKQWGHNVALKPSMGRT
                     QTIQIRDGMLYGASDPRNPDGQTLGY"
     gene            complement(53271..54185)
                     /locus_tag="PK518_00260"
     CDS             complement(53271..54185)
                     /locus_tag="PK518_00260"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HRM47370.1"
                     /translation="MTSSPLKLGFIGLGIMGAPMCGHLIAAGHQLFIHTRSKLPAALA
                     ESSATQCTTARGVAERADIVFLMLPDTPDVEAVLFAEDGVAAGLKGSAAKVVVDMSSI
                     SPVATKDFAKRIEAVGAQYLDAPVSGGEVGAKNATLSIMVGGPDAVFERVKPLFEKMG
                     KNITLVGGNGDGQTAKVANQIIVALNIEAVAEALLFASRAGADPARVREALLGGFASS
                     KILEVHAERMIKRTFDPGFRIALHQKDLNLALSSARQLGVSLPNTAQAQELFNSCVAH
                     GGAAWDHSGMVRALEKMANFEVGQKAPA"
     gene            complement(54240..55040)
                     /gene="hyi"
                     /locus_tag="PK518_00265"
     CDS             complement(54240..55040)
                     /gene="hyi"
                     /locus_tag="PK518_00265"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HRM47371.1"
                     /translation="MPRFAANLSMLFTEVPFLDRFERAAQAGFEAVEFLFPYAHTVEE
                     IKERLDATGLQIVLHNLPAGDWDAGERGIACDPQRVDEFRAGVAKAVSYAHALGVPQL
                     NCLAGKAPAGVDAAVLRRTFVENLRFAAAALKMAKLRLLIEPINPFDIPGFYLNRTDQ
                     AIAILDEVGADNAFVQYDIYHAQRVEGELAATLQKNLARIAHVQLADNPGRNEPGSGE
                     INYPFLFAHLDRIGYAGWVGCEYKPAQGTEAGLGWLEKLAGKKALAPA"
     gene            complement(55067..56857)
                     /gene="gcl"
                     /locus_tag="PK518_00270"
     CDS             complement(55067..56857)
                     /gene="gcl"
                     /locus_tag="PK518_00270"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HRM47372.1"
                     /translation="MAKMKAIEAAVKVLEKEGVSVTFGVPGAAINPLYAAMKEHGGIG
                     HILARHVEGASHMAEGYTRAVAGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIATIAKPVTKWATTVLEPAQVPRAFQQAFHLMRSGRPGPVLI
                     DLPIDVQLAEIEFDIDTYEPLPAYKPAASRKQAEKAIAMLNESERPLLVSGGGVINAD
                     ASELLVELAEILNVPVIPTLMGWGSIPDDHPLMAGMCGLQTSHRYGNATMLSSDFVFG
                     IGNRWANRHTGSVDVYTKGRKFIHVDIEPTQIGRVFAPDYGIVSDAKAALEQFVAVAR
                     EWQAAGRLKDRSSWVAECQGRKNSVDYLRKTHYDNVPMKPHRVYQCMNRNLPKDTTYV
                     STIGLSQIAGAQFLHVYQPRHWINCGQAGPLGWTTPAALGVRVADPTRKIVALSGDYD
                     FQFMIEELAVGAQFKLPYVHVLVNNSYLGLIRQSQRAFSMDYCVQLAFDNINMEEGEA
                     ARGYGVDHVKVVEGLGCKAIRVHRPEDFAAAMTQAEAWMAEHQTPVVIECILERVTNI
                     SMGAEIDNVVEFEELALSGADVPSALAMLD"
     gene            56966..57883
                     /locus_tag="PK518_00275"
     CDS             56966..57883
                     /locus_tag="PK518_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM47373.1"
                     /translation="MDRLDAMHMFVRVVETGSFTQVAREFATTQPTVTKQVAAMEARL
                     KVRLLNRNTRGVSLTEPGTLYYEKCKAIVSDVAEAENVVQVRQHQVQGQLRIGSSVAF
                     GRRVVIPLALDFMQHNPRVEVDLSFEDRYTDLVANGIDVALRLGKLADSTLGARTLGV
                     NPWVLVASPHYLKKHGTPRRPSDLKEHATLIYSSVQGNDVWRLRNASGEAVSVPVSGR
                     LRSNNLSALLAAARAHMGIAALPWYVAHDSLAAGRVVEVLKTCRLPEQEIHAVYPSPR
                     LVPQKVQAFIAFLQGRFGSTWWEQLPKGG"
     gene            58034..58354
                     /locus_tag="PK518_00280"
     CDS             58034..58354
                     /locus_tag="PK518_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HRM47374.1"
                     /translation="MSNPLYENIKRDPRYAQLVRERARFATTLALLVIAVFFGFVLLV
                     AFQPQVIALRLFEGSNLTLGIALGFTQFVFFCALTWVYVRRANSDFDARNAQIVADAM
                     RKTA"
     gene            58351..60033
                     /locus_tag="PK518_00285"
     CDS             58351..60033
                     /locus_tag="PK518_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRM47375.1"
                     /translation="MMQRPISLACRSAAALSLLLPALAIAAPDLGATQKQPLNWHAIG
                     MFFVFVLMTLGITYWAAGRSKSASDFYTAGGGITGKQNGLAIAGDFMSAATLLGLTAM
                     IYGDGVDGYIYMVAFFVGWPIILFLMAERLRNLGKFTFADITAYRLSQGPVRTMAAVS
                     SLTVVCFYLVAQMVGAGQLIKLLFGLDYNVALVVVGVLMMVYVIFGGMVATTWVQIIK
                     ACMLLAGGTAIMLLAFSQFGFSLDTLLTKTMEVHKLGANMLSPGKLLADPVTALSMGL
                     GLMFGIAGLPHILMRFFTVTNAQEARKSVFYASGIIAFFFNVIAIMGLCAIVLVGTNP
                     DFFEGGVVGGKLIGGGNMVALHLAKAAGGNLLLGFLSAVAFATILAVVAGLALAGASA
                     ISHDLYARVIMKGQASEAKEIRVSKMATLGLGVVAVLLGMAFEKMNVAFMVALAFGVA
                     SCANFPVLLMSMYWKGLTTRGALVGGYTGLISSVLFVLFSKSVWVDVLGNKAALFPYT
                     QPALFAMPLAFLAIYVFSRLDSSAQADKERAAFDDQYVRAQTGVGAASAAAH"
     gene            complement(60070..61392)
                     /locus_tag="PK518_00290"
     CDS             complement(60070..61392)
                     /locus_tag="PK518_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRM47376.1"
                     /translation="MTTTPSRPLQDPQAFLLDLYRVAVQRAQPLHSMGQHLPPPPKGR
                     TVVLGAGKAGGSMCQALEALWPQEAPLSGLVVTRYHHIPPRPEGLAQRIELVEAAHPV
                     PDAAGLLAAERMLALTQGLTEDDLVLCLISGGGSALLTLPAEGLDLAEKQRINQALLE
                     SGAAIGEMNCVRKHLSRIKGGRLAAACHPARVVTLTISDVPGDDPSIIASGPTVADAS
                     TCADALAILQRYQIAVPDAVRAALQAGALETPKPGDARFAGHQVHLIATPQQSLQAAA
                     RAAQAAGIAAHILSDEIEGESREVGKVHAALARAVARRDEPFARPCVILSGGETTVTI
                     RPRQPGAAKGRGGRAGEFCLGLAQALQGQEGVWALAADTDGIDGVEDNAGARVAPDTL
                     ARAAQNGQRIGDHLDRNDAYGFFEPLGDLVFTGPTNTNVNDFRAILVL"
     gene            61512..62180
                     /locus_tag="PK518_00295"
     CDS             61512..62180
                     /locus_tag="PK518_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM47377.1"
                     /translation="METSTTSFIVENLTRAIVEHRLLPGTKLAEQKLADHFGVSRTLV
                     RQALFQLSQNRLIKLEPARGAFVATPSVAEARQVFAVRRMLEAEMVRAFMAQSTPAKI
                     RSLRQHVAAEKKAMEREDVGQRTELLGDFHVRMAELMGNEVLAQLLGELISRCALITL
                     MYQSNVAAEHSHDEHADIVEALAGGDADHAVQLMRQHLDNVEAGLTFDRDVPTSDLSM
                     ALSA"
     gene            62204..63154
                     /gene="puuE"
                     /locus_tag="PK518_00300"
     CDS             62204..63154
                     /gene="puuE"
                     /locus_tag="PK518_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HRM47378.1"
                     /translation="MTYDATAPYPRDLIGYGRTPPQPQWPGQARVAVQFVLNYEEGGE
                     NCVLHGDAASEQFLSEMFNPAAYPERHMSMEGIYEYGSRAGVWRILREFERRGLPLTV
                     FGVATALQRHRDVTAAFTELGHEIACHGLKWIHYQGVPEEVERAHMAEAMQIIQGLTG
                     SRPLGWYTGRDSPRTRRLVADHGGFEYDSDYYGDDLPFWMKVQKTDGSAAHQLIVPYT
                     LDVNDMRFALPQGYSHADPFFQYMKDSFDALYAEGDPAGDNAPKMMSIGMHCRLLGKP
                     GRITALQRFLDHIQGHNKVWICRRIDIARHWKERFPAPIL"
     gene            63218..64990
                     /gene="uraD"
                     /locus_tag="PK518_00305"
     CDS             63218..64990
                     /gene="uraD"
                     /locus_tag="PK518_00305"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HRM47379.1"
                     /translation="MALTLEQLNRAPLPEATQMLDGLYEHTPWIAEQALAQRPFTSLA
                     HLKHAMVRVLAAAPREAQLALVRAHPELAGKAMVSKTLTAESTNEQSKAGLTDCTPEE
                     FARIQQLNSDYNARHGFPFILAVRGPRGLGLPKQEIIDTFARRLENPVDFELAEALRN
                     IHRIVEIRLNDKFGFAPTLGNDVWDWQEKLSTHSDPGYAEKGQLTVTYLTDAHRACAQ
                     RISHWMRDCGFDEVEIDAVGNVVGRYHGAQPDAKYLLTGSHYDTVRNGGKYDGRLGIF
                     VPMACVRELHRAGRRLPFGIEVVGFAEEEGQRYKATFLGSGALVGDFRAEWLEQKDAD
                     GITMRQAMQHAGLCVDDIPKLKREAANYLGFVEVHIEQGPVLNELDIPLGVVTSINGS
                     VRFVGEVCGMASHAGTTPMDRRRDAAAAVAELILYVEKRAAQDGDSVGTVGQLEVPSG
                     SINVVPGRCRFSLDLRAPTNAQRDRLVHDIEAELQAICARRFVHYSLEETMRAAAAPS
                     APEWQQRWEKAVAALGVPLYRMPSGAGHDAMKLHELMPQAMLFTRGINSGISHNPLEA
                     TTSDDMQLAVDAFTHVLNQLAQEA"
     gene            65005..66258
                     /locus_tag="PK518_00310"
     CDS             65005..66258
                     /locus_tag="PK518_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM47380.1"
                     /translation="MSNYQQLDAWIDQHFDEQVQFLQALVRVPTDTPPGNNAPHAERT
                     AELIKDYGFEAEKHAVPQAEVQAYGMESITNLIVRRPYGNGGRTVALNAHGDVVPPGE
                     GWSHDPYGAEIVDGAMYGRATAVSKSDFSTFTFAVRALEAVARPTKGTIELHFTYDEE
                     FGGLLGPGWLLEQGLTKPDLMIAAGFSYEVVTAHNGCLQMEVTVHGKMAHAAVPHTGV
                     DALQGATAIMNALYAENVKYQQVTSNMPGIKHPYLNIGRIEGGTNTNVIPGKVVLKID
                     RRMIPEENPAEVEASIHAVIAKAVQDFNQQRDYQGQDAVRTDIRRILLANAMTPLPGN
                     APLVEAIQQHGEAVFGEKPAAVGTPLYTDVRLYVERGIPGVIYGAGPRTVLESHAKRA
                     DERVQLEDLRRATKVVARALHDLMA"
     gene            66501..67988
                     /locus_tag="PK518_00315"
     CDS             66501..67988
                     /locus_tag="PK518_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HRM47381.1"
                     /translation="MTTAVHPVDETLPLGRLTALGLQHVLVMYAGAVAVPLIVGRALN
                     LPPEQVAHLISADLFVCGLVTLIQAWGATQWFGIKLPVMMGVTFAAVAPMVSMAQATG
                     GHAGAGLIFGAVIGAGVVSILIAPMMSRMLRFFPPVVTGTIIAVIGISLMRVGINWIF
                     GNPVGPTAPSVPNPEHLKWLAEAQAAAGAPGSSLAPVPKGFAIVPTIPNPKYADLTGV
                     GISGIVLLTILLIARFGKGFWANISVLLGIAAGGVITAAMGLMDFHKVGKAQWFDIVL
                     PFQIAMPVFDPILILTMTLVMIVVMIESTGMFLALGEMTDKEISQEDLKRGLRTDGLG
                     TLIGGIFNTFPYTSFSQNVGLVAVTGVKSRWVCVAGGVILIILGVLPKMAALVESLPT
                     VVLGGAGLVMFGMVAATGIRILSKVDFQHNRNNAMIVAVSIGVGMIPLVAPNFRQWMP
                     HSIHPLIESGILLSSLAAVALNVFFNGAKHDARAAIEAAKQAEAH"
     gene            68175..70484
                     /locus_tag="PK518_00320"
     CDS             68175..70484
                     /locus_tag="PK518_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRM47382.1"
                     /translation="MNQPLSAAEVALREAALEYHRNPSKGKISVTPTKPLSNQRDLSL
                     AYSPGVAYPCLDIQADPSKAFDYTSRGNLVGVITNGTAVLGLGDIGPLAGKPVMEGKG
                     CLFKKFAGVDVFDIELDARDPDKIIEIVAALEPTLGGINLEDIKAPECFYIEQELSKR
                     MNIPVFHDDQHGTAIISSSALLNGLELVGKQIDQVKVAVSGAGAAAIACVDVMVGLGV
                     KREHVFMVDSKGVIYEGRPGGMDASKARYAQKTEARTLADVVNGADVFLGCSAPGVLT
                     AEMVKTMADKPIILALANPEPEIRPELAKAVRPDCIIATGRSDYPNQVNNVLCFPYIF
                     RGALDCGATKITEAMKLACVRQIAALAKENISEEVANAYAGKELAFGPDYLIPTPFDS
                     RLILKIAPAVAQAALESGVASRPITDMDAYRSSLTRFVYQTGMLMRPVFNAAKSLPKA
                     QKRVAYADGEDERALRAAQFAIDDNVAHPILIGRPAVIAARIAKAGLRMEPGKDVEVC
                     DPSDDPRFRQYWETYYQLMKRDGATPEMAKAAVRRSNTIIASLMVKLGDADAMICGLV
                     GTYETHLERIHHILGHSPDAKQYAALNALMTPNNGTLFITDTYVNEDPTAEELADIAW
                     AAVQEVQRFGIPPKVAFLSHSSFGSSKRASARKMRAARDLFVARHPDIECDGELHGDA
                     ALEPKVRNTYLQDSTLTAPANLLVCPNIDAANILYNVLKTTTSGGVTVGPVLMGTSAS
                     AHILTPAATVRRVLNMTALAVVNSATRNQ"
     gene            complement(70563..71135)
                     /locus_tag="PK518_00325"
     CDS             complement(70563..71135)
                     /locus_tag="PK518_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRM47383.1"
                     /translation="MRSAFLTIAAAALTATAVHAEPATYAIDPTHTFATFEIGHFGAS
                     VNRGRFDKKEGSVQFDKAGRVGKVELTFHINSINTGTAQFDKHLQSAEIFDAAKFPTA
                     QFVGDKFTYEGDKLTAVSGNLTIKGKTQPVTFKANQFACYDSPMLKREVCGGDFETTI
                     DRTAFGADYGVQYGFPKNVRIVAQIEAIKQ"
     gene            complement(71187..71759)
                     /locus_tag="PK518_00330"
     CDS             complement(71187..71759)
                     /locus_tag="PK518_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRM47384.1"
                     /translation="MVFSRHMAWALAGAACLATGPALAQQKLIAENSEIAFVSKQMGV
                     PVEGRFKKFDAQVAFDPAKPATSKVAFTVDTSSATLGVRETDAELPKAVWFNVPKFPQ
                     ATFESTAIKAVGAGRYEVAGKLSIKGAVHDVLVPVTLTQAGANTTAAGSFVIKRLAFK
                     IGENEWADTSMVADDVQVKFKLALTGVPKL"
     gene            complement(71807..72373)
                     /locus_tag="PK518_00335"
     CDS             complement(71807..72373)
                     /locus_tag="PK518_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRM47385.1"
                     /translation="MSPVTTQRPARYGAPAIALHWVLGLAIVVMLVAGWYMTGLPFSP
                     QRLKLYNWHKWAGMAVLALSVLRLVWRFIRRPPALPADMQAAMPRWQQRAQHGTHHLM
                     YGLFFIVPLLGWAYSSAAGFPIVFLGWLPLPDFVPVSPELADALKPLHGLAAYALALL
                     VVLHVAAAVKHHFIDRDGLMARMLPGRG"
     gene            72608..73297
                     /locus_tag="PK518_00340"
     CDS             72608..73297
                     /locus_tag="PK518_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3334 family protein"
                     /protein_id="HRM47386.1"
                     /translation="MNPPNTPVVFGTENILLSLCNSVTRVLSVATQGPVHYSAMVQRI
                     TKTCLKPDIGCFVLFDGGFSGLVIINFSAAAAMEIYERYMLSMGMAREELASSFTSDE
                     VSNVMGELMNQVVGDFTGKVRRELQTHITQNQPKMLVINQQVMLSVDANLDQPEARRV
                     SFYTANNNIFYLELAIDRTQFIKLYDFEPQDAPDPDALMAQAAGAAATATAALPDPDA
                     DTEALLKSLGM"
     gene            73492..73980
                     /locus_tag="PK518_00345"
     CDS             73492..73980
                     /locus_tag="PK518_00345"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRM47387.1"
                     /translation="MAIVVNKPLPEFEANATGGIKVSNVSHQGQILILYFYPKDNTPG
                     CTTEAMQFRDKFKDFEKAGAVVFGVSRDNMKSHDDFKEKLELPFELIADTEEKMCHMF
                     GVVKNKIMYGKKVKGIERSTFLINPEGILVQEWRGLKVPGHVDEVLKAVKAIKAPVKK
                     AA"
     gene            74136..75905
                     /locus_tag="PK518_00350"
     CDS             74136..75905
                     /locus_tag="PK518_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRM47388.1"
                     /translation="MPLPPAPTKRAALLSPDAYRLAAPQEEAPNSADTETLPSATRKR
                     ATSKNKAGSVAGPAITGTATATPAVAAAPAKPAITGPAPVTQARGRPAADKPASDGSD
                     ATPSNVMPLRAAPAGAARKRRATGPKKMFVLDTNVLLHDPTSLFRFEEHDIFLPMIVL
                     EELDAHKKGMTEVARNGRQASRTLDALAAAHGAADMAHGLKLDATGQRAATGSLFFQT
                     APLSYQLPASLPQGKADNQILGVVEALRKLHAPREVVLVSKDINMRVKARALGLAAED
                     YQNDKTLEDGDLLYSGSLALPQDFWSKAGKNVESWQSGVHMYYRIGGPVVAQLMINQF
                     VYFEAPGEPSLYARVTEIRDKTAVLETLKDYGSAKNAVWGVNTRNREQNFAMNLLMDP
                     DVDFVTLTGTAGTGKTLMALAAGLTQVLDERRYTEIIMTRATVSVGEDIGFLPGTEEE
                     KMGPWMGALDDNLEFLAKGDGGNAGEWGRAATNELIRSRIKIKSMNFMRGRTFLNKYV
                     IIDEAQNLTPKQMKTLITRAGPGTKIICMGNLAQIDTPYLTEGSSGLTYAVDRFKGWP
                     HSGHILLARGERSRLADFASEVL"
BASE COUNT        13233 a        26175 c        24249 g        12399 t
ORIGIN      
        1 cgcatgcacg tgcagaaggc actcatggag taccttctgc tcggccgggt ggcgtgaggc
       61 caacgcggtt gcaatcaccc cccgggtagc tcaccatccc ttgctccccc acgacagctc
      121 cagcgctcag gtgcgcgggc tccaccttgc caagtcccat gatgtgcagc acctggcagc
      181 cgtgcgcgag caggtggtcg gcgacgatgc gccgatggca gcgccaccag acggcctcgg
      241 cacacatcag cgcgcagcgt ttttgctcgc tcagcatcag cagctgctgc aggccctggg
      301 caaacgcctg cgtgcaggcg tagtccgcat agcggtgaaa gctggcgttg ctccaccagc
      361 cattgcgctg gccgtccatt cctgggctgc gtccgcgcag gccgcccagc gccgccaaat
      421 gcagatagcc cacgccctgc gtggccaacg cggcggccag cgcgtcggcg ttgaactgcg
      481 gctgcgcgcg cgagccggtc agcttgcgaa tatccaccag gcactgcaca tcgacctgcg
      541 ccagcagcgc cagaaaatcc gccaggctgt ggttggaatg gccgacggtg tagacggtcg
      601 gcatcatggg atgcgctgca gcaccgcgcc gcggtgcgcg gcgatgtgct cggtcttgtc
      661 gctctggatt tcgtactgcg ggttctcggg cgtcgcgcgg tgccggtggc ccttgtagtc
      721 aaaatcctgc gtgtgcacgg cgatgatgcg gccggtcacg aaaccggcct cggaattcca
      781 gcgcacatgc tcgccgatgc gaaaatcctg ccccatggtt tttcctcctg cacgcggcgc
      841 gcgcccatga ccgacgccta ccgccaccac atgatgcgcc cgctgcatcg catgcacgcc
      901 ccgatctgat cgcccggtgg cttgtccgcg ggcgcgtttc ctcctctacc cccacctgca
      961 gccccctcct gacaggcgcc tcgcgctcct tcgggctgtc cgcctggccc ggaaccatgt
     1021 ccgattccgt ccacccctgc ctgacctgtg gcgcctgctg ccacgaatac cgtgtcgaat
     1081 ttcccgtcta cgaactgcaa tccatgggcg gcagcgtgcc cgatgcgctg gcccatgagg
     1141 tgcatggcaa ccgctggcgc atggatggca cgcagcgcca tccggtgcgc tgcgtggcgc
     1201 tgacaggacg ctgcggcaag cagtcgatct gcagcatcta cgaacgacga ccttcgacct
     1261 gcgaccagtt ccagatgggc acagagcgct gcaacgaagc gcgtgcacgc cacggcctgc
     1321 cgccgcttta tgcgccccgg gaagattttc cgctcgcggc ctgatgccaa tttatagggc
     1381 aaaaatgggt tctaacaccc gtccatcaag tgcctacagc tattgatttg attgcaaact
     1441 atcggcgttg cgtgccactg gcgcgggcgg cgccacgctc tgctgctgca ccccggcctt
     1501 ggcccgcgta ccgcagccgc cgcagctgct gcaaccacca ccaccgccac tgccgcagcc
     1561 acccggcccc gcagccagcc cggggtgcac gcggcccagg cgctggcgcc aggcggcagg
     1621 cagatagcgc caggccatat aggccacggc aacgacaacg atcagggcga cgatgagttc
     1681 ttgcaacatg cttcaacctc cgccccatgc cagggccaag cgataggtca cgaagctggc
     1741 gccataggcc agcgcgaaca gataggccgt catcaccaat ggccagcgcc agccgttggt
     1801 ctcgcgccgc acggcggcga tggtcgagac gcattgcggc gcgaagatga accacaccag
     1861 cagcgacagc gccgtggcca gcgaccattg gccggccagc agcggcgcca gctgcgccgc
     1921 cgtgtcgtcg gccgaggcgg caatggcgta caccgtgccc agggcgctca ccgccacctc
     1981 gcgcgcggcc atgccgggca ccagggcaat gctgatctgc cagttgaagc ctatgggggc
     2041 gaagatcatt tccagcacgg cgcccaggcg gccggccagg ctgtattcga tggccgctcc
     2101 ggtggcaccc tcgggcggcg ccgggtaggt cgagagaaac cacagcagca ccgtgacggc
     2161 caggatgatg ccgcccacgc ggcgcagaaa gatgccggcg cgctcccaca ggccgagcgc
     2221 caggttgcgc gggctgggcc agcggtagct gggcagctcc atcagcagcg gcgccacgcc
     2281 ggcactgcgc cgcgccagcc gggccaccgc cgccacggcc atggcgctca ggatgccccc
     2341 caggtacaga gcaaacagca ccaggccctg caggttgaac aggccggcca cctcgcgctc
     2401 ggggatgaag gccgcgatga gcagcgcata caccggcagg cgcgccgagc aggtcatcag
     2461 cggcgctatc aggatggtca ccagccggtc gcgccagtca gggatggagc gcgtggccat
     2521 gatgcccggc acggcgcagg caaagctgga caagagcggg atgaaggagc gccccgacag
     2581 gcccaccacg cccatgatgc ggtccagcag aaaggccgcg cgcggcaggt agcccgactc
     2641 ttccagcgcc aggatgaaga agaacaggat caggatctgc ggcaaaaaca ccaccacccc
     2701 gccgacgccg gcaatcaccc cgtcggccag caggctgcgc agcgggccgt cggccatgtg
     2761 ggcattgagc cattcggcgg ccagcgccgt ggcgccctcg atccagccca tgggcagctc
     2821 ggcccagctg aacaccgcct ggaacatcaa gaacagcgtc accgccagca gcagcgtgcc
     2881 ccacagcggg tgcagcacca cggcgtcgat gcggtcatcc tgctccaggc gctgcgccgg
     2941 ctcgcgcacg gccaggccca tgatgcggcg tacctcctgg tgcaggcgct gcacgcgctg
     3001 cggctgatcc tgctccagcg cgttcagggc cggggccggc gcatcggtcg gcggccgtgg
     3061 ttgtaccggg ccatctggcc tggcctgatc cagccagtgc agcagctcct gcgcgccatc
     3121 ggtgcgcacg cccacggtgg ccagcaccgg catgcccagc tcgcgcgcca gcagcgcacg
     3181 gtctatggcg atgccctggc gctgggccat gtcgctcatg ttcagcacca gcaccatggg
     3241 caggcccagc tcgcgcgcct ccagcaccag gcgcaggttc aggcgcaggt gcgtggcgtc
     3301 ggtgacgcag accagcagct cgggcacggc ctcgccgggg cgccggcccg tgaccacgtc
     3361 gcgcgtcacg gcctcatcca ggctttgcgc atgcaggctg taggcgccgg gcagatccag
     3421 cacgcgcacg cgccggccgc tggcggtggc aaacacgccc tccttgcgct ccacggtcac
     3481 gccggcgtag ttggccacct tctggcgcgc gccggtcagc aggttgaaca gcgccgtctt
     3541 gccgcagttg gggttgccca gcagggcgat atgggtaaac gctgccgccg cagcctggcc
     3601 cgcgctcatg ccgcgcccct cggcacatgc accagcacct gcgcggcctc atggcggcgc
     3661 agcgcaaacg tggcctggcc cacgcgcacg gccagtggat cggccgtggg aaagcccttg
     3721 gcgatgacgc gcaccggctc gccgggcagg aagccgatct ccatcaggcg cagcgccatg
     3781 gcgctggcgc gcccatcctc ggcctggcgc aggccggcca ccacggcggg cacgccacgc
     3841 ggcaggctgt cgagtccctg ggtttgctgg gttgcctggg tatcgccctg gagttgcatg
     3901 ttctatgggt gttgcaaata aaaatggttc tcattttaat gcatcggcgc gccctgggct
     3961 tgatccagct cagcgcctgg caggcactgc acaatcccca tctttcttcc tgtcacccca
     4021 tgagcaccca tccctgcctg agctgcggcg cctgctgcgc cagtttccgc gtcgacttct
     4081 ccgtccacga gaccgaggac atgggcggca gcgtacccca gggcctggtg gtggaggtga
     4141 ccggccacac cgcccgcatg cgcggcaccg accatgcccg gccacgctgc gctgcgctga
     4201 ccggccaggt gggccagcgc gcggcctgcg gcatctacga atggcggccc tcgccctgcc
     4261 gtgagttcga agccggcagc gcagcctgca accgcgcgcg ccagcgccac ggcctggcgc
     4321 cgctggaagg cctggtgcca tgagctacca gcccccgctg tgggacatct cggcgccgct
     4381 gcatgccagc agcccggtct atcccggcga cacgccctac gagcagcgct gggacgcacg
     4441 catcggtccg gacagcgcgg tgaacctgag caccctgacc ctgtcgcccc atataggcac
     4501 gcatgccgac gcgccgctgc actacgacaa cgcaggcgtc gccataggcg ccgtggcgct
     4561 acaggccttc atcggcccct gccgcgtgat ccacgccctg ggctgcggcc cgctggtcga
     4621 atggcgccac atcgcccacg ccgtcagcgc ggacctgccg ccacgcgtgc tgctgcgcac
     4681 ctatgcgagc gcgcccgtcg atcgttggga tgcgcagctg agcgccgtcg cacccgagac
     4741 cctggagcgc ctggccgacg ccggcgtgct gctggtgggc atagacacgg ccagcgtgga
     4801 cccggctgac agccgggagc tgcccagcca ccaggtgctg cgccggcgcg gcctgcgcgt
     4861 gctggagaac ctggtgctgg acgccgtgcc cgagggcgac tacgagctca tcgccctgcc
     4921 gctcaagctg accacggccg atgcctcgcc ggtgcgcgcc gtgctgcggg cgctggcgtg
     4981 aggactacaa atttcaagcg atttatggct ctagagctta cccataaagc gccagtagct
     5041 attaaaaaag aagcgttcac gccgaggcat gatgctcaaa gcatgcctgc agcctgtcgc
     5101 cccaggcgcg cttttcggcc tgcggggcga aactggcctc gaaactgttg tgcgccagct
     5161 gccaggcatg gcgcgccgtg aggatgggca gggccgcaaa ggtctggatg aagttggcgt
     5221 tgatgtagcc gccaaagtag gccgggtcat cggaattcac cgtggccacc aggcccgctt
     5281 ccagcagcgc cggcaggttg tgcgccgcga ggctggggaa cacgcacagc ttgaggttgg
     5341 acagcgggca cacggtgagc ggtatgcgct ccgctgccag gcggcgcatc agcgccgcgt
     5401 catgcacagc ctgcacgcca tggtctatgc gctcggcctt gagcacatcg agcgcgcccc
     5461 agatataggc cggcggcccc tcctcgcccg catgggccac cacatgcagg cccaactcgc
     5521 ggcagcgtgc gaacacgcgg gcaaatttct ctggcgggtg gcccagctcg ctgctgtcca
     5581 ggcccacgcc tatgaatttg tccatccagg gcagtgcctg ctccagcgtg gcgaaggcgt
     5641 cttcctcgct caggtggcgc agaaagctca ggatcaggct ggcggatatg cccagctcgg
     5701 cgcgcgcatc cacgcaggcg cggtgcaggc cctggatcac cgtgtcgatc gccacgccgc
     5761 gcgcggtatg cgtctgcggg tcgaagaaga tttccgcgtg cactacgtta tccatagctg
     5821 cccgcgcaag gtaggcgctg gccatatcgt aaaaatcctg ttccttcaaa agcacgctgg
     5881 cgccggcgta gtagatgtcc agaaaactct gcaggtcggt aaaggcgtag gcgcggcgca
     5941 gcgcctcgac gttggcatag ggcaggtcca cgccattgcg ccgggccagg gcaaagatca
     6001 tctcgggctc cagcgagccc tcgatgtgga tgtgcagctc ggcctttggc atggcggcca
     6061 gcagagccgg caggcgttcg ggagcgatgg tggggattgt cggcatgggg gctttccttg
     6121 gcgtgatggc cccatgatgc cccggcggcc aggccgcgca agagcgtttg caatgaccgc
     6181 gatcggcata gggagcctcc attgtcggat gcagctcgcg ctggctgccg tcgggtttgg
     6241 gaatcatcac cttgcgcacc gggctgggcc ggtaccttcc tgccagcagt gcttggcgga
     6301 tgtcgggcca agtctggcgt atcacctggg cggcccgctc gatgtcgagc ccgtccaccc
     6361 ctgccgcacc cttgttggcc ttgacccgct tccacgcggc ttgcaggttc tgtctcgtca
     6421 gcgccgcttc cagcaagcca ccggtgcctg ccttgggctt tgccgaccct gtgccccggt
     6481 gctgcaggag cgggccgcag gcttcgtcac tgacagagtc acccgcggct tcaccgcgtg
     6541 ctgctcctgc ccgcccggat tgctccggca tctgacgcat ggcctttgac atctcctgat
     6601 ccttggtcac tcactctcgt tcggcccttg gctgtcgcct tctggcctca tcggccccgg
     6661 cttgcagcta ctacgacctc tgctgacttc tggctccggc atgaccgtcg ggctttcacc
     6721 cacaaggcga gatctcccca ggtaagaacg cactccttcg ctgcacagcc gccggatcta
     6781 cgccgcctga gccttgatca cgagagcttt gcggcttcat gcccgctcgc cctgctcggc
     6841 accgccttct atccggttct tgttcatcgg ctcgcagctt cgctccacgc ttcctctcca
     6901 cggtcagtca ccctctcgca gttgcgcttc gcttcgttcg ccgtgatcaa cttacgggag
     6961 gacttgcacc tccaagagtg cgcccatgct gggcgcacat aacaaaggcc gccttgcggc
     7021 ggcctccggg ttcagagggt cagaccaggc ttacttcttg tcgccgcccg gcacctggcc
     7081 gtccacgccc tggacgtaga agttgatgcc ggtgaggaat ttgtcatcgg ccgcagcgtc
     7141 cttgtccagc acggtcttgc cggtgttgtc cacgatgggg cccttccaga tcacgaagct
     7201 gccgtccttc agaccggcct tggcggcatc caccttggcc ttgatgtcgg cgggcacatc
     7261 ctcggcgatg gacaccaggt cgatggcgcc ctccttcacg ccccaccagt tctgtgccgt
     7321 ggcccagctg ccgtccagca cgtcgcgcgt ggccttgatg tagtacggcg cccagttgat
     7381 gacggccgag cccaggtggg ccttggggcc gtaggcggtc atgtcgctgt cccagccgaa
     7441 ggcacgcttg cccttttcct gcgcggtctt gagcacggcg ggcgagtcgg tgttctggaa
     7501 cagcacgtcg gcgccgccgt tgatcaggct ggtggcggcc tcggtctcct tgggcgggct
     7561 gaaccattcg ttgacccaga ccaccttggt cttgatcttc gggttcacgc tttgtgcgcc
     7621 cagggtgaag ctgttcaagt tgcgcagcac ctcggggatg ggcaccgagc cgaccacgcc
     7681 cagggtattg gtcttggtca tggcgccggc gatgatgcca gcgaggtagg caccctcgta
     7741 ggtgcggctg tcgtagatgc gcatgttggc gctggtcttg tagccggtgg catgctcgaa
     7801 cttcacgtcg gggaattcac cggccagctt ctgcatcacg tccatgtagc cgaaggtggt
     7861 gccaaagatc agcttgttgc cctgcgtgac caggtcgcgt agcacgcgct cggcgtccgg
     7921 gccctcgggt acgctttcca cgtaagaggt ctggatcttg tcgccaaact cggcctcgag
     7981 cttcttgcgc gcctggtcat gggcatagga ccagccgccg tcgcccaccg ggccgacata
     8041 ggcaaaggcg atcttcagcg gctcggcctt ggccgcgggt gccgccgcgg gcgcgggggc
     8101 gggggcggcc ggcgcggcct cttccttctt gccgcagccc atcagtgcgg tggcggcgac
     8161 ggccgacagg gcggccagct tgaacaggga acgcttgtgc agatcggtca tgttttcttt
     8221 cagatgatga acacaaaaaa acgcgcctga atgtggtcag gcgcgttgct ggggtttact
     8281 tgccttcggt gccgggcacc ttgccctcca cgcccgccac atagaaattg atgccggtga
     8341 ggaacttgtc gtccgccacc tggccggcgc ccagcagttc cttgccggtg ttgtccttca
     8401 ccgggccggt ccagacggcg aagctgccgt ccttcaagcc ggcgcgggcc ttggccacgc
     8461 ggtccttgat gtcctggggc acgtcgtcgg cgatcttcag caggtcgatg gcgccttcct
     8521 tcacgcccca ccagaagttg ccgctttgcc actggccgcc cagggtgtcg gtgaccacct
     8581 tggtgtagta cggcgtccag tcgatcacgg ccgagcccag gtgcgccttg ggcgcaaagg
     8641 cgctcatgtc gccgtccttg ccaaaggcgc gcatgccgcg ctcctcggcg gtcttgagca
     8701 ccgcgggcga gttggtgttc tggtacatca cgtccacgcc gccgttgatc aggctggtgg
     8761 cggcctcggc ctccttcggc gggctgaacc attcgttgac ccagaccacc ttggccttga
     8821 tcgacggatc gacgctctgc gcgccgagca cgaagctgtt gatattgcgc accacctcgg
     8881 ggatgggcac ggaggccacc acgcccacgg tcttggtctt gctcatgccg ccggcgacga
     8941 tgccggccag gtacgcaccc tcgaaggtct tggtgtcata ggtggccacg ttggcggcct
     9001 gcttgtagcc ggtggcgtgt tcgaacttca cgtccggcag atcggccgcg gccttttgca
     9061 cgaactcctg gtaaccaaag ctggtggcaa agatcagctg gttgccctgg cccgccatgt
     9121 cgcgcatcac gcgctcggcg tcggcgctct cgggcacgct ctcgacgaag gaggtttcga
     9181 tcttgtcgcc aaacttttcc tggatggcct tgcgccccag gtcatgctgg aaggtccagc
     9241 cgccgtcgcc aatcgggctg acgtacatga aggcgatctt cagcttgtcg gccgcgggcg
     9301 cggcggcggc gggagcggga gcggcggccg gtgcggcggc ctcttccttc ttgccgcagc
     9361 cggccagggc cgccagtgca atggccgaca gggcggcggt tttgagcagg gtgcgtttgt
     9421 tcaggtgctg catgggagtc aacggagggg taaaaaatca gctgccgggg tggaaggcct
     9481 tgcccagcga ggccggcata ttggcacgaa tccaggtcgg gttgcgcgag atcaacacca
     9541 gcaccacgat ggtggccaga tagggcagca tggacagcag ctggctggcc agctgcacgc
     9601 cgctggcctg caggtgaaac tgcagcatgg tcacgccgcc aaacaggtag gctcccagca
     9661 gcacgcgtgc cggccgccag gtggcaaagg tggtcagcgc cagcgcgatc cagccgcgcc
     9721 cggcgaccat gccctcgacc cacagcggcg tgtagacggt ggagacatag gcccccgcca
     9781 gcccgcacag cgccccgccg gccatcaccg ccagaaggcg tatgcggcgc accgggtagc
     9841 ccagcgcgtg cgccgaaccg ggcgactcgc ccaccgagcg cagcaccagg ccggcgcgcg
     9901 tgcggtacag aaaccacacc atcagcaaca ccagcaccat ggtcagatac accagcgggt
     9961 ggtgcctgaa caaggcctga ccgaccaccg gcagatcacc cagcaccggc caggagaact
    10021 tgggcgtatc cggcagcttg gcctgcacat agctgatgcc cacaaaggcc gaaaaaccca
    10081 cgccgaacaa cgacagcgcc aaccccgtgg cgtactggtt ggtattcagc cagatcacca
    10141 gccagccgaa cagcgccgcc agcagcgcgc ccgcggccat gccggccagc aggcccagcg
    10201 caaagctgcc ggtatgcacc acggtggcaa aggcggcgat ggcggcgcac agcatcatgc
    10261 cctcggcccc caggttgacg atgccggcct tttcgttgat caggaggccc agggcggcaa
    10321 tcgccagcac ggtgccggcg ctcagcgtgc tgcccagcag caatgcgtac gattccatca
    10381 agccaatccc ttttgtgccg ccgcccagcg cacgcggtag gcgatcagcg tgtcgcaggc
    10441 cagcagcgtg aacagcagca ggccctggaa cacgccggtc agcgatttgg gcaggcccag
    10501 gcgcgactgc gccagctcgc cgccgatgta gaacatgctc atcagcaggg ccgaaaacac
    10561 catgcccacc gggtgcagtc gccccacgaa ggccacgatg atggccgcaa agccgtagcc
    10621 cgccggcacg aagggtgtga gctggccgat cggcccggcg acatccagcg cgccggccag
    10681 gccggccgtg ccgcccgata tcaagagcgc cgtccacagc gcgcgccgcg acgaaaagcc
    10741 cgcgtagcgc gccgccgccg gcgccagccc gcccacctgc agcgcaaagc cggcgcgcgt
    10801 gcgaaacaga aaggcccaca gcagcgccac gccgatgagc gccagcggca ggccgatggt
    10861 cacgcgcgag ccggcaaaca gcttgggcat ctgcgtcacg cgctcgaaca tcttggtctg
    10921 cgggaagttg tagcccatcg ggtccttcca cggcccatag accaggtagc cgagcagcag
    10981 cgtggccaca tacaccagca tcaggctgac caggatctcg ttggcattga agcggtcgcg
    11041 caagagcgcc gtgatgcccg cccaggccat gccgccaagc acgcccgcca ccaggatggc
    11101 cggcacgatc cacggccccg tgcccttgtc ggccagcagc gccacgcccc cggcggccac
    11161 ggcgccgatg acgaactgcc cctcggcacc gatgttccag acattcgagc gaaagcacac
    11221 cgccagcccc agcgcgatca gcagcaacgg cgtggccttc agcgccagct cgcccagcgc
    11281 gtagggcgac ttgatgggct cccagaagaa cacctgcagg ccgcgcagcg ggtccttgcc
    11341 cagcgcggcg aacagcgcca cgccgatcag caccgtgatc gccagcgcca gcaacggcga
    11401 gccataggcc cacaggcgcg aggcctgggg ccgcgcctca agcttgagca tggccgcccc
    11461 cctgatctgc gccgccagcg cccaggtgct gctgcacatc ggcatgccac aggccgctca
    11521 tccattcgcc tatgcgcgcc accgtcgcct cggcacgcgg cacgctgggc gacagccgcc
    11581 ccttggccag cacatgcagg cggtcacaga tctcgaacaa ttcgtccaac tcctcgctga
    11641 tgaccagcac cgcgcagccc ttgtcgcgca gctgcagtat ttcgccacgg atctgcgccg
    11701 ccgcgcccac gtccacgccc caggtgggct ggctgacgat gagcagcctg ggcgcggcgt
    11761 cgatctcgcg gccgacgatg aacttctgca ggttgccgcc cgacagcgac tgggccggcg
    11821 cattcggccc accggccttg acatggaagc gctcgatgat ggcgcgcgcc tggtgctgca
    11881 gctgccccat gcgcagccag ccgccccggc ccaccgcgtt gccgcgcgtg agcagcaggt
    11941 tgtgcgccag gctcatgctg ggcacggcgc cacggcccag gcgctcctcg ggcacgaagt
    12001 gcaggcccag ggcgcgccgg cgccgcgggc tcaggcggcc ggcgttcttg cccgccagtt
    12061 gcaccatggc cggttgcgcg cgctggtctt cgcccgacag cgcgtagagc agctccttct
    12121 gcccgttgcc cgagatgccg gcaatgccca ccacctcgcc ggcgcgcaca tccaggtcga
    12181 tggccagcag cggcacgcca aacgggtcgg tggccggcaa gctcaggttg cgcgtgtgca
    12241 gcaccacttc gcccgcgctc agcgcgcggt gcgtcagccg tggcggctcg gcgccaatca
    12301 tcaggcgcga cagcgaggcg ttggactcct gcgccgggtt gcacacgccc gtcaccttgc
    12361 ccgcgcgcat caccgtgcag gcgctgcaca gctcgcggat ctcgtgcagc ttgtggctga
    12421 tgtagaggat ggagcagccc tcgctggcca gctttctcag caccacgaag agcttgtcca
    12481 cggcctgcgg cgtgagcacc gaagtgggct cgtccaggat cagcagcctg ggcgcggtga
    12541 gcagcgcacg gatgatctcc acgcgctgca tctcgcccac cgagagggtg tgcacgggcc
    12601 gctgcgggtc aatgtccagc ccgtactcgg ccgccgtggc gctgatgcgg cgcgccacct
    12661 cggccagtgg caggcgcctg tcctggccca gccagacgtt ctcggccacg gtcagggtgt
    12721 cgaacaggct gaaatgctgg aacaccatgg caatgccgag ctggcgcgcc tcctgtgggt
    12781 tcttcacctg cacgggccgg ccgtcgaaca gcatctgccc ggcgtcgggc ttgacggcgc
    12841 cgtagatgat cttcatcaag gtggacttgc cggcgccgtt ctcgcccagc acggcatgca
    12901 cctcgccggg ctgcacggtg agcgatatgg cgtcgttggc cagcacggcc gggtagcgct
    12961 tggtgatgcc cgccaactgc agccgcggcg ccgcccgcgt gggcatggcg gcggcatcag
    13021 attggggggg tggattcatg ccgagattgt ctcctagttt tttgtcttgc gattttgaat
    13081 gaaaaaggca tatagcgctt ccccaatgcg tgctgatagc tatttattta atagtatcaa
    13141 cgctccggca cagccccccc gcgccgcagc atcgtgcatc acgcgcgaac gcgcaccgct
    13201 cccaggtgga cgcggttgcg cccggcacac tgggcctcgt agcaggccat gtcggccgcg
    13261 tgcagcacgg cggccgcgtc gcgcagcccc ggcgccagcg gcaccaggcc tatgctcagg
    13321 cccagcgtga agctgcgccc ctggtaggcg ggctcccagg cccgcaccgt ggcacgcaaa
    13381 cgctcggcca cggcctggcc attggccagg ctgctggcgg gcagcagcac ggcgaattca
    13441 tcaccaccca ggcgcgccgc ccagccactg cgctggacct cggcctccag caggcgtgcc
    13501 aggcggcgca gcacatcgtc agccacggcc cggcccgcca gatcattgac cacggtgaaa
    13561 tgatccacgt ccatgaacag cagcacgccc tgctccggcg ccagcgcgcc cggttgcgcc
    13621 gcgcgctggg cccgctcggc cagcagcacc cgaaggcggc gctcgaactc gccatggtcg
    13681 agcagctggg tcagcgcatc gtggctgctc tcccaggcct gctgcgcgcg cgcctcacgc
    13741 tcggcggtgc aatcctgcac ggtccagacc acgccgcgcc cgggctcgcc gggctgcacc
    13801 agctggcctc gcacatggcc ccacaagacg ctgccgtcct tgcgcagcag gggcagttca
    13861 ccgtcaaacg cgccatgcgc ggcaaacgcc tcatgcacct gctgggccgt atccagccca
    13921 tcctcatgcg gggccagcag gtcgatcagg ttgcgcccct gcaactcctg tggcgcatag
    13981 cccagcaggc ggcaaacctg ctggctcacc acctccacct gctcggcacg cgtgaccgca
    14041 atgcccagcg gcacatgctc cagcaccgcc tgcagctggc gcaccagggt gtccgcgccc
    14101 cgctgggact tgcgccggcc ctccagcagc cgggtacaca cgcgcaccac atcgtccact
    14161 tcaccgctgg agcggggcca gggttcgtca gccccctcgg cggtcgaggc cgggcgctcg
    14221 tcggcctcca gcgccgcccg tacgcgcagg cgcagcagcg tcaacggccg cgccagccac
    14281 agcatgaaca gcgtggccag caggcagacg ccgcccgcgg ccaccgccac ctgcccccag
    14341 gcgcggcgct gggcctgctg caacggcgcc aacatggact gggcgtcgct gacccgcgcc
    14401 accatccact gcggcatggg cacgccggcc aggctgacca catgatcggc ctgcggctgc
    14461 gtggccgcgc cacccgcgaa tgtttgcccg gcagcgcgcc aatcggcata ggcctgggcc
    14521 aggccgggct catcgctcac ctggcccagc acacgctcca gattcgggtg cgacaggatg
    14581 accccgtcgc gcgtgaaaat gaccagccgt gaatcgctgc gttccggcaa cgacagcgaa
    14641 tgcggcagca agccctgcga ctgcagccgc agcacgcccg acacgacgcc catgacccgc
    14701 ccctcgccgc gcagcagcgg catggtgaac atcacacgcg catcggtcgg gctattgccg
    14761 atcaggcccg acaccagcgg cttgccgttg accagggtgc gcaccaggta gtcgcgctcg
    14821 gtggggtcga gatccgaggc cttggccagc cggccatagc gcagattcac gctcagggtg
    14881 ccgtccttgc gcgccacctg gatggcatcg aagaagcgca ccgccggcag gccctgctgc
    14941 agcagccagc ccagcgacgc gggcgattca agtatctccg gcgtgatgcc ctcggccacg
    15001 gtggacagca ccttctggct ctgctcgatc ttgctcgcca gcaggcgcgc gaccaactcc
    15061 acctcgtcgt tctgctggct caccagcctg tccatggcct cctggcccat ggcatgcgag
    15121 acccaccagg cagcaagcga gccagacagc gcaatggcgg ccagcgccac cagaagcagc
    15181 ttgcgcacca gcggcccggg gaaccagccc gaggccgtcg attcctcagc ctccggctcc
    15241 cacgccgcgg gttgatgcgc aggcaagttc atacccactc cgcttttgaa gcgcctgtgg
    15301 caagcagatg gaaggtgaag cgctgcatat gggcgggcga gaaaatgacc tcgggccaat
    15361 atacgcagcg cctccatggc ccggcttggg tggaaccctg tgagccacgg atgaaaaccc
    15421 tgacacgcgg caaatgcacg tcatatttga aacaaataga tgcaaaaagc gctgaaaccg
    15481 ccgggtttca gcgcttttgc ctgctgaaag caagacaggg gtggttaaaa ccccgccacc
    15541 atctgcagcg cgcgtggctg cggcaggttc agcacattgc cggtgccggt gatcaggcca
    15601 tgctcgcgca ggtggcgcag cacacgcgac agggtctcgg gcgctatgcc cagttgcgcg
    15661 gcgatcagac gcttgcgctg gtgcagggtg acgcgcaggc cgccatcctc ggcgttgtgc
    15721 gcgtggcgca gcaaccattg ggcgcagcgc gcctcggcgt cctgcgccag gcggctgacg
    15781 gccagctcgg tttgctggca atagccggtg gccatgtcgc gcagcagggc ctgcgccggc
    15841 tcgggcagat cgtcaaagct gcgcttgaag tcctccagcg gcacgcggcg caactgcacg
    15901 cgcgtgtcgg ccaccatgtc cacggcgcag ggctggtcca gcagggccgt ggcggcgtcg
    15961 agccagaacg gcccttcgat cacgccgagc tggtggcgca acacgccgtc ctcgcgcacg
    16021 cccagcagca ctcggccact gtccaggtag agcacggagg cgggttgctg gtggcgctga
    16081 cgcagcactt cgcccgcggt ggccacctcg atctgcacgg actggtctga ggacggaatg
    16141 gggaacatag gcgccactgt gccggcgtac ccacttttgc actttgagga atatcaatag
    16201 tgggttagca cggccgtcaa caatatgcgg ctctttgcat gccatttttg tatacacgca
    16261 acactctgca cccgtacggc cgaaaaaact ggcgacatgg atggcgcgtc accaaaatgg
    16321 cgcgctgaca cgcttgcgcc aggcccggcg ccgtgcaaca ccccccacag gatcgcctgc
    16381 aggcacccgg ccatgccagg ccccaccgga gacaaaccca tgaaccacaa gctgattgcc
    16441 cttgccaccg ccgcgctgtg cgccgccggc gcccacgccc aatcctccgt ccaggtcacc
    16501 ggcctgatgg atgtgtatgc cggctccatg aagatggccg gcgacgccaa gcgcaagtcg
    16561 gtcgtcgaca gcggcggcat gaccacctcg tggttcggct tcaagggcac ggaagacctg
    16621 ggcggcggcc tcaaggccag cttccagctg acctcgttca tccaggtcga tgacggtacc
    16681 caggggcgct ttccgaagga cacctttttc tcgcgcgacg ccaacgtcag cctgtcgggc
    16741 ggttttggct cggtgctgct gggccgctgg atggcgccca acttcctgcc ctcggtcatc
    16801 ggcaatccgc tgggcgactc gttcgagttc tcaccactga tcctgcacaa gaacgtgccg
    16861 ctgttgaacg cctcgaactg gaaggccacc acgccgtccg acaccggctg gagcaaccag
    16921 atcgtctaca gcacgcccaa catcggcggc ttcaaggcca acctgcaata ccagtttggc
    16981 gagcagccgg gcgacaacgg caagaagaac attggcgcca acttcttcta ctttggcggc
    17041 cccctgacgg tgacgggcta ttacgaacgc gaccagatca gcaatcccgc ggttcccaac
    17101 acctacctgg gcacgaccaa gaaggactgg atgctggccg ccgcctatga cttcaaggcg
    17161 gtcaagccct acctgagcta tggccagacc aaggccgaca acagcccgga caaggccaag
    17221 acgctgcagc ttggcgcatc cgcaccgctg ggcggcggca gcctgctggc gtcctgggcc
    17281 aagaccgagc tgtcggcctc caacctggat cgcaagacct tcaccgtggg ctacgactac
    17341 tacctgtcca agcgcaccga cgtgtacgcc atgttcatga acgaccgcat caccagccaa
    17401 ccgacaggca acagcttcgg cgtgggcata cgccaccgct tctgatcggt ttaagctctc
    17461 gcgcacgggg cacgctggct gatggccgcg tgccccttgt ttttgcccct gaccacacga
    17521 agacggagaa gccccccatg cccatgtttg tcgatacctc accacccggc cagtgcatct
    17581 tttgccgact ggtgcagggc agcatccccg ccgccaaggt gtacgaggac gcgctgacca
    17641 tcgccttcat ggacatcggc caggtcacac cgggccatgt gctcgtggcc acccggcgtc
    17701 acgcggccac gctgctggac gccacacccg aagaggccgc cgccgtgatg cagaccgcgc
    17761 accgcgtggc gcgcgccata caggccagct tcgacccgcc gggcctgacc ctgctgcagg
    17821 ccaatggcat actgggctgg cagaccgtgg cccactttca catgcatgtg gtgccgcgcc
    17881 gcgagggcga tggcctgggc ctgacctggc cgcgccagga gccgccggcc cagacgctgg
    17941 ccggctacgc cgaacgcctg cgcgcggcgc tgtgaacccg gtgcgccagc ggtggcaaag
    18001 ccgccgcgcg gcacgcccca ccgcagcttg caccccctga acagcgcctc ttcagcgccc
    18061 attcatacta tattattaat agctgtttgc gcttaactac agtgcgctgc agccattttt
    18121 gatcgtattg gcacgccctg cacggccgat cagactctgc taccctcgcg cgcatcatgt
    18181 ccgtcccgtc cgcagccata ggagcgcagc aggcaccggc cgtgcccgct ggcctggcca
    18241 tcgtggtgct ggccctgctg ctgagcatcc agcccgtcac caccgacctg tacctgcccg
    18301 ccctgcccgc gctcacgcgc ggcctgggtg cggacgtggc cgcaggccag ctcacgctgt
    18361 cggcgctgct gctggccttt ggctgctcgc agctggcatg ggggccgctg tcggatcgct
    18421 ttggccggcg gcccgtactg ctggccgggc tggcgctgta cacgctggcc tctgtgggcg
    18481 cggtgctggc gccgtccatg gatctgctgg tggcctggcg cacggcccag ggcctggcca
    18541 tgggcgcggt ggtgatgtgc gcgcgcgcca tcgtgcgcga cctgtacacg ccgctggcgg
    18601 gggcgcgcgc catgtccaaa gcgctcaccg ggttggggct gatcgcctgc ctgtgcgccc
    18661 cgctgggcgg cctgttgacc gaatggctgg gctggcgcgc ggcgctgcta gtgctcaccg
    18721 cctatgccgt ggccacgctg gccctggtgg ccctgcgcct gcccgagacg ctgggccagc
    18781 gcaacccacg ggcgctgcac ccggcgacgc tggtgcgcac ctgggcactg gtgctgcgca
    18841 cgcccacgtt ctgggccttc tcgctgctga ccacggccag ctatggcggg ctgttcacct
    18901 ttctggcggc ctcgtccttc gtgtacctgg acgtgctggg cagctcgcgc acgcagtacg
    18961 ggctgacgct gctgtccacc gccgcggcct acctgctggg cacgctgctg tgccggcgcc
    19021 tgctggcgcg ccatggcctg aagcgcacgg tggccattgc cgggttgttg agcgccagcg
    19081 gcgggctgct gatgctgctg gccgccgccc tgggctggca cagcgtggcg gcgctgttgc
    19141 cgccgttcta cctgttcatg ctgggccatg gcatccacca gccctgcggg caatcgggcg
    19201 cagtggggcc gtttccgcag gcggctggcg tggcatcggc gctcaatggc ttcatgatga
    19261 tgctggcggc ctttgccatc ggccgctggc tgggcggggc gctggacgga accgtctggc
    19321 cgctggtgca gggcgtggcc ctgtggtcgc tgctgctggc tgccattgcc tggacgctgg
    19381 tgcagcgcca cgccccgcag gagagcgccg atgcaccgtg accccatccc cgccatcgcc
    19441 ctggccggcc ccacggcctc gggcaagacg gcggcggcct tcgccattgc cgaggcgctg
    19501 gcgccacggc tgccggtgga gatcatcagc gtcgattcgg ccctggtgta ttgcggcatg
    19561 gacataggca cggccaagcc cacgcgcgat gagctggccc gcgtgccgca ccacctgatc
    19621 gacatccgcg accccctgca ggcctacagc gcggccgagt tcgtgcgcga cgccacgcgg
    19681 ctgatcaccg agatccgcgc gcgcggcgcc ctgccgctgc tggtgggcgg caccatgctg
    19741 tacttcaagg ccttgctgga tggcctggac gacatgccgg ccgccgaccc cgccgtgcgc
    19801 gcgcgcctgg aacagcaggc cgcagccata ggctgggccg gcatgcacgc cgagctggcg
    19861 cgcgtcgatc cgcagacggc cgcacgcctg gcacccggcg acagccagcg catacagcgc
    19921 gcgctggagg tctggcatgt ctcgggccgg ccgctgtcga gctttcacac tatcaaaaca
    19981 gaagcttata acgcccgccc cataagcgat acaagccttt tttccttgga accagagaac
    20041 cgcacctggc tgcacgagcg catcgcccag cgctttcacg ccatgctggc ggcgggcttt
    20101 ctcgacgagg tgctgcgcct gcgcgcgcgc ggcgacctgc accccgacct gcccagcatg
    20161 cgctgcgtgg gctatcgcca ggcctgggag tcgctggacg gtctgcaccc catggccagc
    20221 ctgcccgaac gcggcattgc cgccacgcgc cagctggcca agcgccagat cacctggctg
    20281 cgcagcatgc cggggcgcca caccatcgcc tgtgacgcgc ccgacgccgg cgcacaactg
    20341 gtgcgcgccg tctgcgcgca tgtgctcggc accggcgcat gaccggggcc gcgccactgc
    20401 tgcaggtgcg cggcctggcc cggcgctacg gcgcacagag cgtcttcgag aacgtccacc
    20461 tggaggtgca cgcgggtgag ttcgtcgcca tcgtcggtga ctcgggcgtg ggcaagtcca
    20521 cgctgctcaa ctgcctggcg gggctggacc gctgggacgc gggccaggtg ctgcaccagg
    20581 gcgtcgatct gggcaggctg gacgataccc agcgcgcgct gtggcggcgc agccatgtgg
    20641 gctttgtgtt ccaggccttc catgtgctgc cgcacctgga cgtggcgcag aacgtcggcc
    20701 tgccgctgct gctgcagggc cgccccgatg ccgcgcgcgt cgggcagatg ctggccgccg
    20761 tggggctgga cggcctgggc gaccggctgc cgcaccagct cagcggcggc cagctgcagc
    20821 gcgtggccat tgcccgcgcg ctggtgcacc gccccgccct gctgctggcc gacgagccca
    20881 ccggcaacct cgaccccggc acggcggcac gcatcatgga tctgctgctg gcccagaccc
    20941 gggagcatgg tgcagcgctg gtgctggtca cccactcggc cgcggcagcg gcgcgcgccg
    21001 cccgcgtgct gcacctgacg gcccagggaa tacgctgaat ccagggaaat ccatgacctg
    21061 gttggctaca tcgaccgcat aaaccctggg ttgcgctacc gcaatagtag ataacatcag
    21121 cggtcgctaa tatatggagg gacaacccca tggcccagat acaggcccag cccgcacccg
    21181 ccagccaaga gcgcgggcgg ctgcgcaagg cacccgagca ttttcttgac gccacgggcg
    21241 tgcgcaccgt gttcgccgag ggcaggcagg ggcgccgcaa cttcatccgc agcgcctttg
    21301 ccgccgccat ggcgggcagc gccgccagcc atgcggccgc gcaagccagg gacacgggcg
    21361 atggtgaccc cgccatcctg aacctgcccg cgcacagcac cggcctgggc cagcccgtgg
    21421 ccaccgatgg ctacggccgc ccctcgcagt acgagggcaa tgtacagcgc cgccagagcc
    21481 cggggctgac gcagaccaac caggcctcgg tgtcgtttgc gccgctgcag tcgctgtttg
    21541 gcatcgtcac gcccagcggc ctgcacttcg agcgccacca ccagggctgg tgggacgtcg
    21601 atcccgccca gcaccggctg atgatcaacg gcatggtcaa gacccccaag gtcttcacca
    21661 tggacgagat catgcgcctg cccagcgtgt cgcgctttca cttcatcgaa tgcggcgcca
    21721 ataccgccgt ggactggggc aacgtggccg tgcccacctg ccagtacacg cacggcatgc
    21781 tgtcgtgcag cgaattcacc ggcgtgtcgc tgatcacgct actcgaacat gccggcgccg
    21841 acctgaaagc ggccaagttc gtgctggccg aaggggcgga cggctcgtcg atgacgcgca
    21901 ccatccccat gagcctggtc accagcggcc aggtgctggt ggcctatggc cagaacggcg
    21961 agatgctgcg ccccgaaaac ggctaccccc tgcgcctggt ggtgccgggc gtgcagggcg
    22021 tgagctgggt gaagtacctg cgccgcatcg agctgggcga ccagccctgg gccagcaagg
    22081 acgaggccat ccactacatc gacctgatgc ccgacggcca gcaccgccag tacaccagca
    22141 tccaggagtg caagagcgtc atcaccaccc cctcgggcgg ccaggtgctg ctggacaaag
    22201 gcttctacaa catcaccggc ctggcctggt cagggcgcgg caaggtcaag cgcgtggacg
    22261 tgtccgtcga tggcgggcgc aactggcgca cggcgcggct ggagacgccg gtgctcgaca
    22321 aatgcctgac gcgcttcaac ctggactggg tctgggacgg caagcccacc atcgtgcaaa
    22381 gccgggccat ggacgagacg ggctatgtgc agcccagcta caaacagctg cgcgaggtgc
    22441 gcggcacacg ctccatctat cacaacaacg ccatccagtc ctggttggtg caggaaaacg
    22501 gcgaggtgaa aaatgttcag attgcgtgac accctcgccg ccgcgctgct ggccgcgggc
    22561 gtcgccctgc cggccctggc gcaggacaag ttccccggca tcggccgcgc cgccaccccc
    22621 aaggaggtgg ccgcttggga catcgacgtg cgccccgact tcaaaggcct gccacccggt
    22681 aagggcacgg tggcccaggg gcaggacatc tgggaagagc gctgcgccag ctgccatggc
    22741 ttttttggcg agtccaactc ggtcttcatg ccgctcgtgg gcggcaccac cgccgatgac
    22801 gtgaagaccg gccgcgtcgc caccctgcgg cgcaccgact accccggccg caccacgctg
    22861 atgaaggtgc ccacggtttc cacgctgtgg gactacatcc accgcgccat gccctgggac
    22921 aagcccaagt cgctcaagcc cgacgaggtc tacgccgtca ctgcctttct gctcaacctg
    22981 gcgcatgtcg tgcccgacag cttcacgctg tccaacgaca acatcgccga agtgcagcaa
    23041 aaaatgccca accgcaacgg catgaccctg gcccacgccc tgtggcccgg caaggacatg
    23101 cccggcctgg cgacccaacc cgacatacag gccaaggcct gcatgagcaa ctgcggcccc
    23161 gagcccgtgg tcacgtcggt gctgcccgag catgcgcgca acaaccacgg caatctgcgc
    23221 gagcaaaacc gcaccgtggg cgcgcagcgc ggctccgaca ccacccggcc cgagggctgg
    23281 ctgggcgacg ccgccggccc ggtgaaggtg gtgggcccgg ccagccacaa cagccccaat
    23341 gccgccgcca tcgcgctcac ccacaagcac gcctgcaccg cctgccacaa catggagggc
    23401 aagctggtcg gcccgagctt tgccgacatc gccaagaaac atggcggcaa ggccgactac
    23461 ctggcagaga agatcaagtc cggcggctcc ggcctctggg gcgaaatccc catgccgccg
    23521 caaaccctgc cacaggatga caccggggcc attgccgcct ggctggccgg tggcgccggc
    23581 aagtgacagg cagcgggcgt tgacacacat tccatcacca cacgcaaatc cgcgttcgct
    23641 tcaatccaag gagtacctct tcatgcaaac cacacgcagg caagtcctca agcacagcgc
    23701 cgtcgttgcc ggcctgttgg cgggaacggg gctgttcccg caatacgcgc tggccttcaa
    23761 caaggccgcg ttcgacgcca agagcctggc ggacgcgctc aaggccctgg gcgccaacgg
    23821 cgctcctgaa gccagcagcg ccgtgaccct cacggcaccg gacatcgccg aaaacggcgc
    23881 cgtggtgcca ctggccgtgg ccagcacgct gccgggcatc aagcagctgg tgctgctggt
    23941 cgagaagaac ccgaacgccc tggtggccgt gttcaacatg ggccccgagc tggaggccag
    24001 ctttgccacg cgcgccaaga tgggggaatc gtccgacgtg tacgcggtgg ccatcaccgg
    24061 cgacggcaag gcgcacttcg ccaagaagga agtgaaggtc accctgggtg gctgcggcgg
    24121 ctgaccagca ccagcactag caccagcacc cctatcaaga aatcaaggag aaacgacatg
    24181 gcagatccga tgcgcatccg cgcccaggca cagggcgaca aggccacggt acgtgtgctc
    24241 atgagccacg aaatggagtc cggccaacgc aaggacgctt ccggcaatct ggtgcccgcc
    24301 tggcatatcc aggacgtgag cgccagcctg aacggcaagc aggtgttttc ctgcgaatgg
    24361 ggccccgggg tctcgaagaa tcccttcctg caattcgtgg tcaagggcgc caaggccggc
    24421 gacaagctca gcgtcacctg gaaggacaac aagggcgaga gccgcaccga cgaggccacg
    24481 gtgtcctgac ccatctctcg cccgggcggg ccgcgctgca ccaggccagg ccccgctttc
    24541 ttgctaccca cggaggagac aaacaccatg cgaggcaaat ccatcgcgtt gatcacggcg
    24601 gccctgggcc tgatgggcct atcgggcctg gctctggcac agaagacatc gatagaggcc
    24661 atagaggagt accgcgagat gctgcaggac ggcaacccgg ccgacctgtt cgaggccaag
    24721 ggcgaagacc tgtggaagaa aaagcgcggc cccaagaacg cgtcgctgga aggctgcgac
    24781 ctgggcctgg gaccgggcgt ggtcaaaggc gccttcgtcg agctgccgcg ctggtttgcc
    24841 gacaccggca aggtgcagga tctggagtca cgcctgctca cctgcatgag cgcgctgcag
    24901 ggcatagacc ccaaggtcat catcgacggc cagtggggca agggcgagcg tgccaacacc
    24961 acggccctgg ccacctggat tgccgcgcag tccaagggca tggccttcca cctgccgcaa
    25021 ggccatgcgc aggagcgcac catgtacgaa gtgggcaagc gcctgttcta ccagcgcggc
    25081 ggcgcgcatg atttctcctg cgcctcctgc catggcgagg cgggcaagcg catccgcctg
    25141 caagacctgc ccgacctgcg caaggccccc ggcgacggca taggctttgc cgcctggccc
    25201 gcctaccgcg tctccaacgg ccagatgtgg agcatgcagc accgcctgca cgactgctac
    25261 cgccagcagc gctttccgga agtggtgttt gccagcgacg tgaccgtggc cctgggcgtg
    25321 ttcctgggcg tgaacgccac gggcgacaag tcggccgtac ccaccatcaa gcgttgaacc
    25381 gcaggaggat gaccatgaag ccacagatca ccaccgcctg cctcggcctg cttgccgcgg
    25441 cggcactggc cggttgcgcc ggcggcagcc gcactgccgg cctgccctcg cccgccgagc
    25501 tggatgccct gacgcagaag gccgtgcagg aatcattcaa ggaccacggc atcgtcaagg
    25561 tgaaaaacgt cttcgccgac gacgaaacca tcagcgcgtg caacgccgcc gacgcggccg
    25621 gcaagccgct ggacgacgcc accgccaagc gcctggaggc cctgaacatg aagaccgtgc
    25681 agtggcccag cgatggcaag ttcctgggca gctggaagga gggggaaaaa ctggcccaga
    25741 gtggccgcgg cctgaccttc accgacacga ccaagacgcc caacggcggc aattgctaca
    25801 actgccacca gatcgacaag aaggagatct ccttcggcac cattggcccc agcctctacc
    25861 actacggcaa gctgcgcggc gtgaaagacc cgaacgcgcc ggaagcccgg gccatggtcg
    25921 aatacacctg gggcaagatc tggaacagca aggcctacag cgcctgctcc aacatgccgc
    25981 gcgcgggcca catgggcatc atgaccaaaa gccaggtggc cgacctggtg gcgctgctgc
    26041 tcgaccccca gtctcccgtg aatcaataag cttttcagca accgtttgcg gccttggctg
    26101 cggtggaaac ccgccgcgcc caaggccgct gccacatacc agccccgcga gcccgccatg
    26161 aacctgacca aacgcgaatt cctgcaagtg ctcaccgccg ccagcgccgc cggcatgggc
    26221 ctgggccgct atgcccacgc cagcaccgac agcgccgccc agggcctgta cgacataccg
    26281 aagttcggca acgtctcctt cctgcacatc accgactgcc acgcccagct caagcccatc
    26341 tactttcgcg agcccagcgt gaacctgggc ctgggcgaca tgcaggggcg actgccgcac
    26401 ctggtgggtg aacaactgct gaaggccgcc ggcatgaaga acggcacggc ggcggcgcac
    26461 gccctgacct acctggattt tgagcaggcc gcccagcgct acggcaaggt gggcggcttt
    26521 gcccacctgg cgaccctggt caagcgcatg cgcgccagcc gccccggcgc gctgctgcta
    26581 gatggtggcg acacctggca gggcagcggc acggcgctgt ggacccacgg ccaggacatg
    26641 gtggacgcct gcaagctgct gggcgtggac gccatgaccg gccacttcga gttcaccctg
    26701 ggcatgcagc gggtgcagga gatcgtcgag aaggacttcg ccggcaaggt ggacttcgtg
    26761 gcgcagaaca tcaagaccac cgactttggc gacccggtgt tcaagcccta cgtgattcgc
    26821 cagatcaacg gcgtgccctg cgccatcgtc ggccaggcct ttccctatac accgatcgcc
    26881 aacccgcgct ggatggtggc ggactgggag ttcggcatcc aggacgagaa catgcaaaag
    26941 gtggtcgatg aggcccgcgc caagggcgcg caggtggtcg tcgtcatcag ccacaacggc
    27001 atggacgtgg atctgaagat ggccagtcgc gtgcgcggca tagacgccat cctgggcggc
    27061 cacacgcacg acggcatgcc cgtggccagc atcgtgaaga acgccggcgg ccagaccata
    27121 gtcaccaacg ccggcagcaa cggcaagttt ctgggcgtgc tggacttcga cgtcaagggc
    27181 ggcaaggtgg cggacttccg ctacaagctg ctgccggtgt ttgccaacat gctgccgccc
    27241 gacaaggaca tggacgcgct catcaccaag atacgcgccc cctacgaggg caagctggcc
    27301 gagcaactgg ccatcaccga gggcctgctg taccggcgcg gcaacttcaa cggcacgggc
    27361 gaccagctca tcgtcgatgc gctgatggag gtgcagggcg ccgatctggc gttctcaccc
    27421 ggtttccgct ggggcacgac gctgctgccc ggccagccca tcacacgcga atggctgctg
    27481 gacatgacgg ccaccaccta cagcttcgcc accctcaccg agatgaaggg cgagatgatc
    27541 aagacggtga tggaggacgt ggccgacaac ctgttcaacc ccgacccgta ttaccagcag
    27601 ggcggcgaca tggtgcgcgt gggcggcctg acctatgcct tcgacccgct gcagggcatg
    27661 ggcaagcgcg tcagcgacat gcgcctgaag ggcgagctga tcgacccaaa caagacctac
    27721 aaggtggccg gctgggcccc cgtggccgag gatgccgcca aggccggcaa ccccatggtc
    27781 tgggagctgg tggagcaatg gctgaaggcg cgcggcggca aggtcacagc gcgccagccg
    27841 aatgtgccgc gcctgacggc cggcctgccc aaccccggct acgcagactg acgcagccgc
    27901 gtcagccgcg gtagagccag ggctgatcga gcaggcgctc gcccagcacg cccatggccg
    27961 cgtcgcgccc ccagcgcagc gggccgctgg catgaaaaat ttgcccgttg cgccgcgatc
    28021 gttcctgcac gcgggcgttg cgctgccagc ggttcaaggc atagcggcgc aggcacaggg
    28081 ccacatccac atcgtgcatg gcgagcgatt tttccagctc caccgcatcc tcgatggcca
    28141 tgccggcgcc ctgggccagg taggggcgca tggggtgggc ggcgtcgccc agcagcgcca
    28201 ccaggccgcg cgccatctcc tgcgggccgg cgacgggcgg gcggtcgagc agcggccaca
    28261 ggcgccagcc ctcccccacc tgaggcacga cgcgtaccaa gtcctgcagc ggcgtacagg
    28321 tctgcgccag ggcggattcc agatcggcgg cgttcacgcg gtggtcccag gagtccagat
    28381 cggccggcgg ctggccatgg cgtatggcca ccaggttcag cagctcgccg cggcgcagcg
    28441 ggtactgcac cacatgcagg tttggcccca gccagacggt gacgtcattc gaacgcagcg
    28501 cctggggcag cgcatcctgg cgcagcacgg cgcggtaggc cagatggccc gacacgcgcg
    28561 tggccgcctc gcccagcagc tgcgtgcgcg tgccgctgcg caggccgtcg gcgccaatca
    28621 gcgcatcacc ctcgacctcg ataaccttgc cgtcaccgcg caccaggcgc acggtgacgg
    28681 cgtcgccctg ctcctcatgt cgttccacct ggtgatccag gttcagatgc accagcgatg
    28741 acttgcgcgc cacatccagc aacaggccgt gcagatcggc acggtggatg gtggcgtagg
    28801 ccgcgccgta gcgctcgacc gcggtggcgc ccaggcgcag cgcgcccagc tcggcgccgg
    28861 tgagcgcgct gcgcacccgc agccgctcgg gaaagctggc caccgccagc agggcccggt
    28921 gcaggcccca ggcctgcagg cgccgcacca cattcggacc gatctgcacg ccggcgccca
    28981 cctcggtgaa tgcgccggcg cgctcgaaca gccgcacctc ccaaccggcg cgcgcactgc
    29041 ccagcgcggc agccagcccg cctatgccgc caccggcaat caagacttgt ttttgcatgc
    29101 cgtgattgtc ggcgcaggcg cggcccgggt tagctgctca gcaactgacg cagcacgtag
    29161 ggcaggatgc cgccggcgcg gtagtagtcc acctcgatgg gcgtgtctat gcgcagcgtc
    29221 accgccacct cctggcgcga gccatcggcg cggcggatca ccagcagcgc ctggctttgc
    29281 ggtgtcaggg cagcgtcggg cagcacgtcg atgatctcgt cgcccttgag gcccaggctc
    29341 tcccacgagt caccggcacg cagctgcagc ggcagcacgc ccatgcccac caggttggag
    29401 cggtggatgc gctcgaagct cttggccacc acggccttga tgcccagcag ctgtgtgccc
    29461 ttggccgccc agtcgcggct ggagccggtg ccgtactcct cgccggcaaa gatcaccgtc
    29521 ggcgtcttgc tggccatgta ctgcatggcg gcatcgaaga tggccatctt gctgccgcgc
    29581 tgcgggccat cgccctggta cagggtcatg ccgccctcct cgcgcgagcc atcggccagc
    29641 gccgggatca tcaggttctt gatgcgcacg ttggcgaagg tgccgcgcat catcacgtcg
    29701 tgatggccgc ggcgcgagcc atagctgttg aagtcggcct tggccacgcc atgcgccttg
    29761 agccagaggc cggcgggcga gctctccttg atggagccgg cgggcgagat atggtcggtg
    29821 gtgatcgagt cgccaaacag ggccatgatg cgcatgccgg tgacagattc agagtgtttt
    29881 tggcctccag cgccctcccc ttgctcgcca acagctacag tatcaagagc aaaatcggca
    29941 aagaacggcg gctcggcgat ataggtgctg gcgggccatg agtacacctc gccgctcacg
    30001 ccgtggatct tgtcccacag cgcgccgggc tcggtcttga ccttggcgta gttctcgcgg
    30061 aaggccttgc cgttcatggc gaacttcagc agcgcctgca cctcctcggc cttgggccag
    30121 atgtcgccca ggtacacgtc cttgcccccc ttgcccttgc ccacaggctc ggtcatcaag
    30181 tccttgagca tggtgccggc aatggcatag gccacgacca gcggcgggct ggccaggaag
    30241 ttggccttga tgttgggatg gatgcgcgcc tcgaagttgc gattgcccga cagcacggcg
    30301 gcgcacacca ggttgtgctg ggtgatggcc tcgttgagct gtggcgtcag atcgccggca
    30361 ttgccaatgc aggtggtaca gccgtagccg gccacggtga agcccagctt ttccagatag
    30421 ggcagcaggc cggtttccgt caggtactcg gtgacgatgc gcgagcccgg cgccagcgag
    30481 gttttgatgt gcggctgcac cttcagcccc gcctccacgg ccttcctggc cagcagaccg
    30541 gcggccagca gcacgctggg gttggaggtg ttggtgcagc tggtgatggc cgcgatcaac
    30601 acatcgccgc tgcccaccgt gagccggtcg ccctggtggc cgtgcatggg ggcgtccaca
    30661 tgctgcacct ccaggcgcgg cttgttgtgc accatctcgg cctcgaagcg cggcgcgccc
    30721 tcggtcgccg ggcgctcgtc gggcacggcc tgcgcggcat cgggtgcatg cgccgcaacg
    30781 gcatggcgct catgcaggcg ctcgggcgcc aggttgaagc cggcctgctc cacgggcttg
    30841 gaaaacaggc tggtgaactg ctgcttgacc tggcccaggt cgatgcggtc ctgcgggcgc
    30901 ttgggtccgg ccaggctggg cgtgacgtcg gcaagattca gccgcacgac ctgtgaatag
    30961 tcgatatcgc ccgcgcgcgg aatgccgaac aggccctggg ccttgaggta ggcctccagg
    31021 gtttcaatct cgtcctgcgt gcgcccggtg ccctcgaaat agtcgagggt ggcctcatcc
    31081 accgggaaga agcccatggt ggcgccgtac tcgggcgcca tgttgccgat ggtggcgcgg
    31141 tcgggcagcg acagcgagcg cgtgccctcg ccaaaaaatt cgacgaactt gcccaccacc
    31201 ttggcgcggc gcaactgctc ggtcacggtg agcaccaggt cggtggccgt cacgccctcg
    31261 cgcagctggc cggtgagctc gaagcccacc acgtccggcg tcaggaagta cagcggctgg
    31321 cccagcatgg ccgcctcggc ctcgatgccg cccacgcccc agccgaccac gccaatgccg
    31381 ttgatcatgg tggtgtggct gtcggtgccc accagggtgt cggggtagta cacgccatcg
    31441 gcgctcttgt gcacaccgcg tgccagatat tccaggttca cctggtgcac gatgccaaag
    31501 cccggcggca cgacgccaaa ggtgtcaaag gcctgcatgc cccatttcat gaactcgtag
    31561 cgctcgcggt tgcgctgaaa ctcgagcttc atattcagct gcagcgcatt cttgctgccg
    31621 tagtaatcca ccatgatgga gtggtccacc accagatcca cgggcaccag cggctcgacc
    31681 tttttcggat ccttgcccag gcgctgcgtc acgctgcgca tggctgccaa atccaccagc
    31741 agcggcacgc cggtgaaatc ctgcagcacg acgcgggaga gcacgaaggg aatttcgtcc
    31801 ttgcgcgctg cattggcgcc ccagctggcc agttgccgca catgctcagc cgtcaccttg
    31861 cgcccatcgc aattgcgcag caccgattca agcacgatgc gaatggaaac cggcaggcgg
    31921 ctcacacggg gatacttgtg tgccagctcc ggcagggaat agaacttgcc tttcttgcct
    31981 gacgccgtgg tgaaattctt gacggtggaa gcaaaggcgt ggcttggggt ggtggtgaca
    32041 gccatacagc aactccggtt gggaaggaca acgtcccatt ctggcaggga gcgacggtat
    32101 cgccaatgat cagggccaat acctttggcc cacgccatgg ttaaaaatgg cgatcggcat
    32161 gcaaatccgg cacatctgcc gcgatcaggt gccagtcaat gcatgtcgtg gccaagcggc
    32221 gccggttgtg ccgttctgtc ccaataaaca aaaaacccgc caaaggcggg tttttaccga
    32281 gggcaaacca agagggcctg cgccacggtg ccaaaggcca gcccggggcg ccggccatgc
    32341 cggatcaaat ggcgaacttc tcacgatcct cattttgaat ccacttcggt gccttgccac
    32401 ggcccgtcca ggtctggccc gttgccggat cgcggtactt gggcgccacc ttcacgccga
    32461 tactggcaga agtgcgcgcg ccacgcccca cgggaaagac ctcctgctgc gtcaaaccaa
    32521 actcggcaat caaaccgcgc acctgggcaa cggcggcggc caattcgcga tcgcgcgctt
    32581 cactgatctg cagttcaagg gcttcacgct gcttcaacag gtctttatag ctggggttca
    32641 tgatagtttg cttgggttga gaattaatag acggcgcgat tatagataaa taattccgtt
    32701 catgtaacga gtttatcggc atgaatgact gatgaatgtt ggtgaacccg gcaagtcatc
    32761 acgcccgtcc gctcattgaa tgagtattca ccaacttcaa tggtcagaaa gcatttgtcc
    32821 accacacccg attcacccgt tcgcagcgcc tgcctgtccg gtgcgggaca atcgccccat
    32881 tcaaaaccac catcgacgat taacacatgg cgctttactg tgcgggcgat atccagggct
    32941 gtggcgatgc tttcgagcgg ctgctgtccg tgatcggctt ttctccgagc cgcgacacca
    33001 tttatctgct gggcgatctg gtcaatcgcg gtcccgcctc ggccgcggta ttgcgccgct
    33061 gcatgcaaat gggcgacagc gtgcgcgcgc tgctgggcaa ccacgacctg cacctgctgg
    33121 ccacggcgca tggcgtgcgc cccccttcac ggcgcgacac gctggacggc atcctgcagg
    33181 cgagcgaccg gggcgcgatg ctggactggc tgcgccagca acccctggca cgccaggtgg
    33241 agcatggcgg cgagcccctg ctgctggtgc acgccggcgt gctgccgcag tggtctgcgg
    33301 acgatgttct ggcgttggcg cgcgaggtgc aggcgcagct tagcggcccg gggttggctg
    33361 aattcctgcg ccggatgtat ggcaaccacc cggatctatg ggacgcgggc ctgcagggcg
    33421 cggatcgcct gcgcatcatc gtcaacgcac tgacccggct gcgcttttgc acgcccgagg
    33481 ggcgcatgga tttcgacagc accgaaagcg ccgcgcaggc gcccgaagga ttggtgccct
    33541 ggtttgatgc gcccgggcgg cgcacccgcg gcacactgat tgcctttggt cactggtcca
    33601 cgctgggctg gatcagccgg ccagacatcc tcgggctgga cacgggctgc gtctggggcg
    33661 gcgcactgag cgccgtgcgc tttggcgcca cgctggccga gcgcgagcac tgcaaggtgc
    33721 aatgcccaca agcccagcgg ccaggagagt agttcgctat aaaattaaaa gctgcctacg
    33781 ctttatcaat accactttca ggcaataaat agccaaaaaa catttgccat caagcgcaaa
    33841 cagctatgct ttttatgcag caattgactt gagcgcagcc gccatgccgc gcgactgcca
    33901 cagcgcgcca tcgctgcccg cagccagcac ggccacgtcg gggtggccag cgctccaggc
    33961 aggtacggcg gccatgccct gcaccatcag cgccgtgccc cagccgttga cggcccgcac
    34021 gtcacgcgcc agcagcgcca cgccatgcac gcccgatgtc ggccagccgg actgcggatc
    34081 cagcacatgg tgcaggcgcc ggccctggtg catgaagcca cgctcgtagt cacccgagga
    34141 ggccacgatg ccgaagccct cgacctcgat ggcgcccagc agcttctcgg gggcgcgcgg
    34201 atcgcgcacc ccgacacgcc aggggcgccc ctgcaggcgg ccgctggcga gcacgtcgcc
    34261 gccgccgttg acaagcgcgt tggcaacgcc ctcgcgctcg agcacgcgca ggcccgcagc
    34321 cagaatgggc aacttggcga tgccgcccag gtcgagctgc atgccgcgct cgctcagata
    34381 ggccgtgccg gcgcgctcat ccaggcgcag cttgcgtgcg tccacatggc gcagcgcggc
    34441 cgcgatgtct gtggcggcgg gcatggcctg ctggcccgcc tcgaaatgcc agccggacaa
    34501 ggcccccacg gtgggatcga aggcgccagc gctctcgcgc catacctgct gggcgttgcg
    34561 cagaatggcc agcacctcgg gcgacacggc aaccgggtgg cgcccggccg cgtgcccgat
    34621 gcgccgcacc acgctgtcct cgcggtagcg gctcagcagc gcctcaaggc gctgcatttc
    34681 gtcaaaggcc aggcctatcg cggcctgcag atagcgcgca tcgccgccgt cggcgacgat
    34741 gtccacgcgc gtgcccatga ggttgcggct tgcacgctgc ggccctgcgg cctgcgccag
    34801 gcctgggctg gcccaggcca cggcgcccag caggggcagc gccagggcga agcgccgccg
    34861 cgacagctgc ccgccttgcc atccgttgtt catggcacgc actccttgca ttacatcgac
    34921 tggtcggcca tgaagtccac ggcagccttg acatcgtcat cgctgagcga ggatgcgccg
    34981 ccgcgtgcgg gcatcatgcc cttggcgccg gtgaagccct cgatggcgtg cttgtacagc
    35041 gtgtccttgc cctgggcgat acgcgggccc cagtcttcct tgtcgcccgg cttgggagca
    35101 ccggccacgc cggcggcatg gcacatggcg caggtcttgc cgtagacgct cttgccggca
    35161 gtgttttccg ccaccggggc gggtgcggga gcaggcgcgg gtgccgcggc gggcgcctcg
    35221 gcagcgggtg ccggggcggg cgcgggtgcc ggcgcctcgt tcttgccgca ggcagccagc
    35281 aacagcgttg cggcggccag cgccatggtc agagatgtct ttttcatgaa agcctttctt
    35341 gtacggaaac gttggtaagc cccaagggcg agtgctgcag acgcaccatg cggcgcgcca
    35401 caccccttct ggcatggacg ccagtttggt gttttccaga attcactatt tgatttacat
    35461 caacatcaaa aggaacattt ggagtgaaca tttatacacc gtcgccaaag tggtgacccg
    35521 tatttccgcc accagaacct gcaccaccgt catggacttc ctccgcctcc tgaatcgcca
    35581 gcgccggtgg atcgtggcct ggttcttcct gagcctgggc gcggcgctgg cggcgcccat
    35641 ggtgcaagcg cgcaccatgg agctggtctg caccggcggc aatggcttga gcctggtcgt
    35701 gcattccagc gacggcaagg ccgcacccga caccctgggc atggactgcc ccctgtgcct
    35761 tctgggcgcg tcggcaccgc cgcctgtccg ccccttgccg caggccgcac ccctgcccca
    35821 ctacacattc gctccaccgt cagtagcgca tgccctgccc cccatggctg cgccaccgcc
    35881 ggcgcgagcc cccccggttt tcccttccgt ttacccatcc tgaaaagagg acttcatgag
    35941 cgacaagaaa gacagcctga gcaccgaaac cgccggcatt ggccgccgcc gtttcatcaa
    36001 caccgccgcc ctggccggcc tgaccgtggg cgttgccgcg tgcaacgaca agcccgcggc
    36061 agcccctgcg gcatccgctc cggcacccgc acccgcgcct gccgcggcca gcggcgctgc
    36121 tgccagcacc cacctcaagc ccggtgagct cgacacctac tacggcctgt ggagcggcgg
    36181 tcataccggc gacatgcgcg tgctgggcat gccctcgggt cgcgaactcc accgcatccc
    36241 ctgctttgtg cctgatgccc tggtcggctg gggcgtcacc aacgaatcca aggcggtcat
    36301 gggcaccaag cccgatggcc acctcaagta cacggtggcc gacacccacc acacgcacgc
    36361 ctcctacaag gacggcaact acgacggccg ctacgcctgg atcaacgaca agatcaacag
    36421 ccgcatcgcc cgtatccgcc tggactattt cgtctgcgac aagatcacgc aactgcccaa
    36481 cgtgcagggc ttccacggca tcttcccgga caagcgcgac ccggtcgatc cggccatcaa
    36541 ctacaccacg cgcgtgttct gcggcggtga gttcggcatt cccctgccca acaacaccgg
    36601 catcgaggac ggcagcaagt accgctcact gttctcctgc gtggacgcag agaccatgga
    36661 agtgcgctgg caggtgctga tcgacggcaa ctgcgaccta gtggcaacct ccttcgatgg
    36721 caagctggcc gccaccaacc agtacaacac cgagagcggc gtgcactatg aggacatgat
    36781 gtccgccgag cgcgacgcct gcctgttctt caacgtggcc cgcatcgaag aagcggtcaa
    36841 ggccggcaaa ttcaaaacct ttggcacgtc caaagtaccc gtggttgacg gcacacgtga
    36901 ttccaacaag gatccgctca ccgcgctgac ggcgtacgtc agcgttccca agaacccgca
    36961 cggtgtgaac gccagccccg accagaagta cttcatctgt gccggcaagc tctcgcccac
    37021 caccaccacc atcgagctgg tcaaggtgct ggactggttc gacggcaaga tggaaaaact
    37081 cgacgatgcc atcgtggccg aagtcgaagt gggcctgggc ccgctgcaca ctgcctttga
    37141 cggccgcggc aacgcctaca ccacgttgtt cctggacagc cagatcgtca agtggaacat
    37201 cgaaaaggcc atcgccttcc acaagggcga caagaacgcc aagtacgtgg tggatcgcat
    37261 cgacgtgcac tatcagcccg gccacatcaa cgcgtcacag tccgagaccg tggccgcgga
    37321 cggcaagtac ctggccgtgg gctgcaagtt ctccaaggat cgcttcctgc ccgtgggccc
    37381 gctgcacgcc gagaacgagc agctgatcga catctcgggc gaaaagatgg tgctgctggc
    37441 cgaccacccg gtgcgtggcg agccgcatga cttcatcatc ttcaagcgcg atctgctcaa
    37501 gcccaagcaa gtgtatgacc tggacgagtc gccactggcc atcaaggatc ccaaggagtc
    37561 cggcgtcaca cgcaatggca agaaggtcac ggtcaagatc acctcgcagg ccccggcttt
    37621 cagcctgcgc gagttcaagg tcaagaaggg cgacgaggtg actctgatcc tgaccaacct
    37681 ggacaagatc gaagacctga cacatggctt cgccatcccg aactacaacg tcaacttcat
    37741 cgtcaacccg ctggagacca agtcggtaac cttcatcgcc gaccagcccg gtgtgttctg
    37801 gtgctattgc acgcacttct gccacgcgct gcaccttgaa atgcgcacgc gtatgatcgt
    37861 cgaggcataa gcctctcgcc aacagtgggc gctgcgctgg tagcgcagcg cccatttttt
    37921 tcaggatagt gtgatgtctg cttttggcca gcgatttgcg ttcttctggg ccgccctgct
    37981 tctcgtcctt gccctggcgg ccccgcgcgc ccatgcaggc gcctatgagg cggaattgcc
    38041 cgccaacctg gcaaccacac ccgacatgtg ctcgctgctg ccctgcggcg aggtgtttcc
    38101 tggcgccaag catttttccg aacgcaaggg ccagcctcct tacgtggagg cctacgacag
    38161 cgacggagcc gacaagaagc tgctcggcta cgtaatgctg tccaccgaca tcacggacac
    38221 ccccgcctat tccggcaagc ccgtggtgac gctgatcggc atggacaagg aaggccattt
    38281 cgtcggcatc caggtactca agcattccga gcccatcctg ctgctgggca tccccgaatc
    38341 ggccctgatc aacttcaaca accagtacct gggcaagtcg gtcaccgaca ccatcgaggt
    38401 cggcccctcg cgccccgatg aaaacgtgat tggcgttgat gccatctcgg gcgccaccgt
    38461 caccgtggtg gcgcaaaacc aggtggtcat ggcctcgggc gcggccgtcg cgcgccagac
    38521 cggcatcatc gcccccacgg tacgcgagcc ggcgcgtttt atcgccggtg gccagaacta
    38581 cgactgggcc cagctggtgc agatgggcgt ggtcaagcaa ctgctggtga aacccgaaca
    38641 ggtgggcctg ccgcgcggcc ccgagccgtt catcgagctc tggtacggcg acctgaacca
    38701 ccccgacgtg ggcaccagcc tgctgggcaa gcacaactac gagcggctgc atgccagtct
    38761 gaaggaaggc gaaaacgccc tgttcgtgat caagacggcg ggccaggaat cgttcaaggg
    38821 ctcgggcttc gtgcgcggtg gcatttacga ccgcgtgcag gtcaagcaag gggcggactc
    38881 gttcaccttc cgcgacctgg attcgctcaa cctctatggc ctggaggccc agggtgcgcc
    38941 gcgctacacc gagtcggcga tcttcatcat ccgctcacat tcgttctcgg ccgcctatcc
    39001 gtggaagttc gccttcctgg gtaaccgcgt cgatcgcgcc acgggccagc gcagctttgc
    39061 cgtgttcgag tccaagtact ggctgcccgc agccctgctc gaaggcggcc gccccaaggt
    39121 cgaggagcct gcagccccct gggtgcgcat ctggaagtcc caggccctgc cgatcagcct
    39181 gttcgccctg ctgctggtcg ccgtgaccgt ggtctacgcc ttgcgcgaga agctcacgcg
    39241 gctgtccacg cacaagaaca aatggccggt gaatggcttc aagtacacct tctgggccat
    39301 cagcatcttc tggatcggct ttggctacat ggcacagccc tccatcaccc aggtgctgac
    39361 ctggttccat tcgctgctgt tccagtggac ctggtcgctg ttcctgtccg atccgttcat
    39421 cttcctgttc tggatcttca tcatcgtcac cgtcttcctg ttcggacgcg gcctgttctg
    39481 tggctggatg tgtccgttcg gctcgctgca ggagggcatc tacaagatcg ccaaggcggt
    39541 gggcctgaag cgcttccaga cggcgctgcc gcagaaatgg catgaccggc tgaagtgggt
    39601 caagtacgcg gtcttcttcg gtttgctggc cgtctccatg ttctccatgg gcctggccga
    39661 gaagctggcc gaggtcgagc ccttcaagac caccttcctg gtcggcgtga tgaaccgcgc
    39721 ctggccctac ggcctgttcg tggccgccat cctgggcgtg tccatcttca tcgagcgccc
    39781 atactgcaaa tacatctgcc cgctcggcgc gtcgctggcc atgcccagca ccttccgctg
    39841 gtttggcctg aagcgcaagc aggactgcaa cagctgcaag gcctgcgccg tgggctgcgg
    39901 cgcgcaggcc atcgacgccg acggccgcat cgaccaccgc gaatgcctgc actgcctgga
    39961 ctgcatgatc ctttacaccg acaccaaggg ctgcccgccc ctggcgatgg agcgcaaacg
    40021 gcgcgagcgc gatggcctgg agatcacgcc cattggcaag aacggctact tcatccccat
    40081 tcacccggcc aaggtggaag accagatcag ccccaaggca aagaagggcg tcgatccgcg
    40141 catgcccacc gaccgcaccc tgcccgcgca caaggaagat gtgggcctgc tggaatggct
    40201 gtggctggag ctgcgcgacc acctctggcc ctggagccgc gagggctggc acagccagaa
    40261 gaccctgcag atcgccggcc tcgcgctggc catcgccgcc accattgcct gggtgcaggc
    40321 cgccctgggc cacctgtcct cgggcgctat catcggctgg tggtttggct ggagcgtgta
    40381 cgaggtgttg attcgtctgt ccggtcgccg ttatgtgaag gatggcccat ggtggcgcga
    40441 ccagtaccgc gtcgccggtg tgatggacat gatgagttac gttggattca agaacctgat
    40501 gattggcgct gcgctgttcc tggtgctcaa gaccctgggc tggctcatcg catgacaatg
    40561 ctgccccatt tagctgcggc cctggcgctg gccatcgccg gggcctgcca ggcagccacc
    40621 atctcggtgc gccccggcga cgatctggcc gaggccgtca ccaaggccca ggctggcgat
    40681 gtgatcgagg tggcgcgcgg ccagtaccgc gccaacctgc tgatcgacaa gccgctcacg
    40741 ctgcgcggcc tcgatcgccc caccatcagc ggcggcaacc agggcgacac catacgcgtg
    40801 acggcgccgg acgtgctcat cgagggcctg atcgtgcgcg actcgggcga cagcctgaaa
    40861 gaccagaacg ccggcatcta cctctacccg ggcgcgcacc gcgccatcgt gcgccgctgc
    40921 gacctgacct acaacctgtt cggcctgtgg atagagaagg ccaacgacgt gctgatcgag
    40981 cacaacacca tcaccggcaa gcgcgaatac gcctcgcccc agcgtggcaa cggcgtgcag
    41041 ctgtacaaca ccaagggcgc caagatcctg aacaacgaca tcagcttcgt gcgcgacgcg
    41101 ctgtatgtcg atgtctcgca caacgcgctg ttcaagggca acaagctgca ccacagccgc
    41161 tacggcacgc attacatgaa ctcctactac aacctgtggg aggacaacga cacgttctac
    41221 aaccgcggcg gcctggcgct gatggaggtg cgccaccagg aggtacgcaa caaccgtgcc
    41281 tggggcaact cggaccacgg catcatgctg cgcacgctgc aggattccga catctcgggc
    41341 aacgtggtcg ccggcaacaa ccggggcttc ttcatctacg acgtggagta catcaagctc
    41401 aacgacaacc tggtcgtgga caacgtcgtg ggcgtgcacc tgtcggccgg ctccacgcgc
    41461 aacgaggtcg agggcaacga cttcatcgcc aaccgcgagc aggtgcgcta cgtgggcgcg
    41521 cgcgacgagg tctggggcaa gcagcgtggc aactactgga gcaactacct cggctgggat
    41581 cgcaacaacg acggcaaggg cgatgtgcag tacgaggcca acgacatggt tgatcgcctg
    41641 acctggcgtc accccagcat caagctgttg ctggccagcc cggccgtgca ggcgctacgc
    41701 ctggtgggcc agcagtttcc cgttttgcgt gtgccctcgg tggtcgatcc ccacccgcgc
    41761 atgcgccctc atcatcaaga ttggagtcag tggcgtggca agcatttccc cggccagtaa
    41821 cgcgccgggc atggcgattt ccctgcgcgg cgtgcgcaag cattttggtt ccctgcatgc
    41881 cgtggacggc gtggatctgg acatccccca gggcgagatc tttggtctga taggccacaa
    41941 cggcgccgga aagagcaccc tgttcaagat gatgctgggc ctgctggccc caaccagcgg
    42001 cagcattttg gtggccggca cgcccgtggg cgggcgcaac ttccgcgccg cgcgccgcca
    42061 gctcggctac ctgcccgaga acgtggtgct ctacgacaac ctggacggcc tggagaccct
    42121 gcactttttt gcccgcctca agggcgcgcc gctggccgaa tgcccggcca tgctggagaa
    42181 agtgggcctg gcccacgccg gcaagcgccc ggtgcgcgaa tattccaagg gcatgcgcca
    42241 gcgcctgggc tttgcgcagg cgctcctggg caatccgcgc gtgctgtttc tggacgagcc
    42301 caccaacggc ctggatccgc aggcgatccg cgacttctac gccacgctgc gcgacctgca
    42361 gcagcagggc gtgacggtga tcatcacctc gcacatcctg gccgagctgc aggagcgcgt
    42421 ggaccgcctg gcgatcctcg tgtcgggcaa ggtcgaggcc ctgggcagcg tgcatgagtt
    42481 gcgcgagcgc accggcctgc cgatcatcgt ggaggtggac ctggcgcccc aggactgcca
    42541 cgacgccctg catgtgctgc accaggtcac cggcgtgaag ccggcgcaga ccccgtccgg
    42601 gctgcagctg caatgtccgc gcgagcacaa gatggcagcc ctggccgcgc tcacgcccct
    42661 gggcgcacgc gtgcaggacc tgcgcctgca cgagccctcg ctcgaagaca tgttcttcgg
    42721 catctccgct taaggcccgc tctcgggcaa tgctattcat ggaactgacc cctatcctca
    42781 ccgtcgcggc caaggagttc cgcgaccgca tgcgcaaccg ctgggtgctg gccgtggccc
    42841 tggtgttcac cgtgttctcg ctggtcattg cctactttgg cggcgcccag cagggccagg
    42901 tgggctttcg ctccatcgag ttcaccatcg ccagcctggt gagcctggtg atctacctga
    42961 tcccgctgat cgccctgctg ctgggttttg acgccatcgt cggcgaacgc gagcgtggtt
    43021 cgctggacct gctgctggcc ctgcccatca cgcggctgga gctgctgctg ggcaaatacc
    43081 tggggctggc gctggcgctg tccctgtcca ccgtggttgg cttcggtctg gtggccgttc
    43141 tgctgtggcg ccacatgaat gccaatgcgc tgtttcacta cggtggcttc atgctgtcct
    43201 cggtactgct gggcctggcc tttctgagcc tggcggtgct gatctcggta ttggcgcgcg
    43261 agcgcacgcg tgcctcgggc ctggcgattg ccacctggtt cttctttgtg ctggtgttcg
    43321 acctgctgct gctgggcctg ctggtcacca ccggcgggca gtttgccggc gacagctttg
    43381 cctggctgct gctgctcaac ccggcggacg tgttccgcat cctcaacgtg ttctcgctcg
    43441 aggacgtgcg caccctgtac ggcctggcca gcgtcgtgcc ccagtcgctg ggcaatccgc
    43501 tgaccatggg cggcgtgatg gccgcctgga tagctctgcc gctcgctttg gccaactgga
    43561 gattcaagcc atgacctgtt cctgcatcac ccgccgccgc gccctgggtt tcgccgccct
    43621 ggccgcactg tccaccgccg gcctgctctc gggctgcggc gacaaggcag ccgagcagac
    43681 atcgctggcg cccgtggaga tcgaccgcag caccagttgc gagctcgacg gcatgctgct
    43741 ggccgactac cccggcccca aggcgcagat ccactacgcg ggccaggaca agccctcctt
    43801 cttctgcgac acggtggagc tgttcaacac cctgctcgcc ggcgagcagg tgcgccccat
    43861 acgcgccgtc tatgtgcagg acatgggcca ggcccaatgg gaagagccca agggccactg
    43921 gatagacgcc aagagcgcca tctacgtact gggcagcaag cgccacggct ccatgggccc
    43981 gaccattgcc agctttgccc aggaagccga cgccacgaaa ttcgccggcg agcacggcgg
    44041 caaggtgctg cgctttggcg acatcaagct agacatggtg gacctgagcg gcggcgcgct
    44101 gcacgacacc cgcatgtaag ccatgcccgc cgcgcccctg ctcccgccac tacagcagcg
    44161 ccgcaggcgc gcgctggcca tgggcgtggt ggccgctctg ggcgttctgg ccgccagcac
    44221 accctggagc gtgccggcgc tgcgccagat gctgtcgccc gaggcaccag cctcgctgga
    44281 gcccgatgtg tgcatcgtcg ccccgcccac accctacgac ccggccagcg gcctgccacc
    44341 cacggccgcg cgcgccgtac cccccgatgt gcgctgcccg gtctgcggca tgttcccggc
    44401 acgctcgccg caatgggcgg cgcagatcat cttcagcaac ggcgacgcgc agtatttcga
    44461 ctcgcccctg tcgctgttca tgtacctgca ggatgtggcg cgctacagcc ccggccgcag
    44521 cagcgggcag atcgcggccc actacgtgac cgacgcctcc gcaccaccgg acggcaagcg
    44581 cacgccctgg gtcgatgcgc tgtcggccta ctacgtgcat ggctcgtcgg ccaaggggcc
    44641 catgcgcgcc ggcaacctgc cagcctttgc aacacgcgcc gcggccgatg ccttcgccga
    44701 gcggcgtggc ggcgtggtgc tggcctttgg tgccatcgac gccgcgctgg tggccgagct
    44761 cgccagcggc ggcggacata gccatcacca ataatttact ataaaaaaaa tagctgtcag
    44821 cgctttatgc ataagcgtta acagctattt ttatcaaata aacctccggg gggggtcccg
    44881 gccccctcac tgggccgtgg gttcggcctc cggcacaggt gccttgaaca gcgccgccag
    44941 cttgcgcctg ggcttgatgt tggccgatat gctgccgcgt gccggcgctg ccttgggcac
    45001 cacctcccag ctgggtggca ggctgtcggc ggtggcggcc tcataaggct tgtcgaagaa
    45061 cgggtcacgc accaccgccg gggccgaacg atagctgctg cgttcgcggc cgccgcgtgg
    45121 cgcaccccgg ttgtcttcgc catcttcacg ccaggcgcgt cggccgtcgt tgaagcggcc
    45181 cctggggcgg tcttccgcaa gttccagcgg ctccagctcg atcttcttct tgatgagctt
    45241 ctcgatgtcg gtcaccaggc gcgtatcgct gctggccacc agcgtcacgg ccaggccgga
    45301 ggcgccggcg cggccggtgc ggccgatgcg gtgcacgtag tcctcggcgt tgaagggcac
    45361 gtcgtagttg aacaccgccg gcacgtcctt gatatcgagg ccgcgcgccg ccacgtcggt
    45421 gcacaccagc agatccacct cgccgctctt gaaggcctcc agggccttca ggcgctcgtc
    45481 ctggctcttg tcgccgtgca gcgccgcggt cttcaggccg tcgcgctcca ggctgcgtgc
    45541 cagtcgcgcg cagccgagct tgctgttgac gaaaacgaag gcctgcttga gcccgcgctc
    45601 ggtgagcaca tggcggatgg cgcggcgctt gtcgtcgtcc tgcgcgctga agaaacgctg
    45661 ctcgacggtg gatgccgtct cgttcgggcg cgcgacctcg atggtcaccg ggtcctgcag
    45721 gtagctgccg gccaggcgct tgatctcggg cgagaaggtg gccgagaaca gcagcgtggt
    45781 acggctcttg ggcagatagg acaggatgcg ctgcaggtcg ggcagaaagc cgatgtccag
    45841 catgcggtcg gcctcgtcca gcaccacgta ttcgacctgg ttcaggactg cgttcttggc
    45901 ctcgatatgg tccaagaggc gcccgggcgt ggccaccagc acctcgacgc cctttttcag
    45961 ctcgatggtc tgcggcttca tgtccatgcc gccaaacacc acggtgctgc gcagcttggt
    46021 gtacttggcg tacagcgcta tctgctgcgc cacctggtcg gccagctcgc gcgtgggcag
    46081 cagcaccagg gcgcgcacgg ggtggcgcgc gggcgaggcc gagctgtttt cgtgcttgag
    46141 cagtcgctcg agcagcggca gcgagaacgc cgccgtcttg ccggtgccgg tctgggccgc
    46201 acccatcacg tccttgccgg tcagtaccac ggggatcgcc tgggcctgga tcggcgtcat
    46261 gttctcgtag cccatgtcgg ctacggcgcg cttgagcggt tctgccagcg ctagggagga
    46321 atatgcttgt gtcatcaagc cgctattgtc gcactgcagc aaagcaaagc gccagggcgc
    46381 cggccgctgc gccccaatcc acccccgaaa agcaccgcaa aaactacaac gcctgcgcct
    46441 tgaaggtgtc gcaggcctgc acgctgccgg tcttcaggcc ctgggaaaac cagcgcacgc
    46501 gctgggcgct ggagccgtgg gtgaaggcgt cggggcgcac ggtgcctgtc tgctggcgct
    46561 gcagcgtgtc gtcgccgatc tgctgggcgg cgttgatggc cgattcgatg tcgctttgat
    46621 ccagccagtt cttggcctgc tgcgaatgcc gcgcccagat gccggcgtag cagtcggcct
    46681 gcagctccag gcgcaccgac agcgcgttct ggtcacgctg gctgatgcgc ccgcgcatgc
    46741 cgtccacctt ggccgtggcg cccaacaggg tctgcacatg gtggcccacc tcgtgggcga
    46801 tcacataggc acgggcgaac tcgccgggag cacccagctg gcggctcatg gtgtcgaaga
    46861 agtccatgtc caggtaaacc ttctggtctc ccgggcagta aaacggcccc atggcgcttt
    46921 ggccggtgcc acaggccgtg ggcgttgcgc ccctgaaaag caccagcttg ggctggcggt
    46981 actgcgcccc accctgctgg aagatctggc cccagacatc ctcggtggaa gccagcacgg
    47041 tggagacaaa cgccgcctgg cggtcatcgc ccggcgggcg ctgggccggc gcctgctgca
    47101 cctggggcgc atcggcgccg gagagcacgc caatggtggt cagcgggttg ataccaaaca
    47161 cgccccaacc gatcaaggcc aggactaccg tgccaatgcc gatggagcgc ccgccgccaa
    47221 agccgccgcc gccaccgctg ctgcggcggt cttccacgtt gtccgattcg cgattgcctt
    47281 cccatttcat tgactgtctc ccgtgcgcgt caggcgctca gcggtgcatg ttacgcccgc
    47341 catccgcgcc tgcgcggcac cggccggccg tacgcgaggc tggtcacgaa gttcaacaag
    47401 tttcacattt gcggcgcgcg gcgctgcggc ggcgtcatgc cttcggcaag gtcacgccat
    47461 gctggccctg gtacttgccc ttgcggtcct tgtagctaac agcgcattcc tcgtcgccct
    47521 ggaagaacag cacctgggca cagccctcgc cggcgtagat cttggccggc agcggtgtgg
    47581 tgttggagaa ctccagcgtc acatagccct cccactcggg ctcgaagggc gtgacattga
    47641 cgatgatgcc gcagcgcgca taggtgctct tgcccaggca gatggtgagc acgtcgcgcg
    47701 gaatgcggaa gtactccatg gtgcgcgcca gcgcaaagct gttgggcgga atgatgcaat
    47761 agtcgccctc gaagtcgaca aagctcttct cgtcgaagtt cttcggatcg accacggtgc
    47821 tgtggatgtt ggtgaagacc ttgaattcgc gcgcgcagcg gatgtcgtaa ccgtagctgc
    47881 tggtgccgta gctgatgatc ttcttgccgt cctgctcgcg gatctggccg ggctcgaagg
    47941 gctcgatcat gccgtgctgc tcggccatgc gccgaatcca cttgtcgctc ttgatgctca
    48001 tgacggtgat actcctggcg cgcattctag cgatcactcg atcactcata aaaaaagagc
    48061 tgctcgcgct tgactgacaa gcgctgcagc ccgttttcat tcaaaccgca gattcagggc
    48121 gtaaccgttg ccccgccctc gtaccaacgc ttgaaggtat ccacctcctc gaccgacagc
    48181 gcgcccgggt tgccaaacgg catcttgcgc agctgcacca cctgctggta gatctgctgg
    48241 gcatggccct tgacctcgtc gggggtgtcg aacttcacgt tcttctgcgc cacggtattc
    48301 gcgctgtggc agaccacgca atgcttttgc atcaccgcct gcacctggcc atagccggcc
    48361 ggctgggtag ccgcgccaga ggccattgca gcggcggcct gcggtgccgg cttgagccag
    48421 cccagcaccc ccagcagcac cagcacgccc acggtggcat agggccaggg atgggtgttg
    48481 cggcccagct tgaagccgtg gcgcatgacg aagaactggc ggatggcggc accggcgaac
    48541 atcatcagga tcagcaccag ccagttgagc tcgtggttgt agagccagcc gtagtggttg
    48601 gacagcatgg caaacagcac cggcagcgtg aaataggtgt tgtgcacgct gcgctgcttg
    48661 gcgcgcttgc catgcatcgg gtcgaccggc tgcccggcct tgagcgccgc caccaccttg
    48721 cgctggccgg ggatgatcca gaagaacacg ttggcgctca tggacgtagc cagcatggcc
    48781 cccatcagca ggaaggccgc ctgacccgga aagatatggc aggccaggta ggccgcaatg
    48841 cacaccagca ccagcaccat ggcgccgacg gtggcgtcac cgttgggctt ttggccaaag
    48901 gcgcggcaga tggcgtcata cagcagccag aacaccacca gaaagcccag cgccgcgaca
    48961 atggccatgg agctggacat cagcgggttg gatgggctca ccaggtagat gccggcattc
    49021 cacaggtagg acaccgtgag cagcgcaaag ccgctgagcc aggtggtgta gctctcccag
    49081 tagaaccagt gcaaatggtc gggcatcttc ggcggcgaca cgttgaactt gacagggtgg
    49141 tagaagccgc cgccgtgcac ggcccagagc tcgccgctga cgccgtcgcg cttcatgccc
    49201 tcgtcctcgg gcggcgtgag gctgctgtcg agaaagacga aatagaacga cgagccgatc
    49261 caggcgatgg cggtgatgac atgcaaccag cgcagcagca ggttggccca gtccaaaagg
    49321 tagctttcca tagtgtctca acctcggcag gcgttcgtgg cctgcccgaa agctgctcac
    49381 cactgcgggt gctctgagca gaaaaacaat gggccgcgca ccttggcatg aagctaccga
    49441 gacaccgctg cgaccgactt tgtggacatt aagtgtatgc agtttttgcc gccaactcca
    49501 cgggcggaaa cccgggattt cccgaagtgg gttgtattag tttgtgccaa gccgctgcaa
    49561 cagctgcgcc gccacggcca cggcgatcac ctcgggctct ttgccggtga tgccagaaac
    49621 gccgatcgga caggtcacct gggccagctc ctgatcggag aacccgcgcg ccagcaggcg
    49681 atgcgagaag gtggcccatt tggtgcggct gccgatcagg ccgatgaagg gcaggtcggc
    49741 gcgctcgcgc tggcgcgcca ggcaggcggc gacgatgtcc agatcctcgg catggctgaa
    49801 gctcatgatg agcaccatgg actgcggcgc cagctcgggc acggcggcct gcaccgggtc
    49861 ggaatgctcg cacagcgtgt ccgcatccac ctgggccggg aacacctcat cgcggctgtc
    49921 tatccagtgc agggcaaatg gcagcggcgc cagcacgcgc gccagcgcat ggcccacatg
    49981 tccgccgcca aacagcgcca ccggctgcag ccgcggcgcc aggcgctggc gcagggccgg
    50041 cgcatcggcc gcatccacgc actcgaaacg cagatgcacc acgccaccgc agcattgccc
    50101 caggctcggg cccagtgcct gccgcagcaa aggcgggcgc gccggcaggc cggccagatg
    50161 ctcggccagg cgttcgcgcg cctcctgcat ggcctgcagc tccagatggc cgccgcctat
    50221 ggtgcccagc gccggcgtct gtcccatgaa caaagccatc caggcaccca cctcgcgcgg
    50281 caccgagcct tgggtagact ccaccgtcac caggcacgcg ggggtggtct tgatgcgctc
    50341 cagcagacta ttcaatgccg ccactcgtgg cctcccgtca gccatgtacc aagactgcgc
    50401 gccacagcat agcgccaccc accatgcctt tgaaaccgtc caacaccccg cgccatgcca
    50461 ggcgcccctg gtggctgccc gccagcctga tggcggcggt ggccgccatc gtggcggcct
    50521 gcaagtccgg cccccagcct ccgccagcac aggatgcgcc gccgccccag ccagccggca
    50581 cggcggccgg cccggtgcgc agctcggccg ccgccacgcc gcgcgcctac cgcagggacg
    50641 ctgccagcca cctttacggc ctgaacccgc agcgcatcta caagggcaag ctgccgccac
    50701 tgctgtacgc gattggcgtg ctggaggtgg acatagaccg catgggccgc gtgtccgccc
    50761 tgcgctggat gcgcgcaccg cgccacgcgc cggaggtgat ccgcgagatc gagcgcacgg
    50821 tgcgcgccgc ggcgcccttc cccgtgcccg cgcgcatgca gcgcgtgacc tacaccgaca
    50881 cctggctgtg ggacaagtcg gggcactttc aactcgacac gctgaccgag gggcagctct
    50941 gaagcgtcac gagccgcggt aagtcgagta gctccacggg ctgaccaaca gcggcacgtg
    51001 gtaatgctgg tcggtatggg ccacgccaaa gtccaggctg acctggttca gaaaattggg
    51061 ctcgggcagc tgcacgccgc gcgccttgaa gtagcccgcc acatcaaagg tcaggcgata
    51121 ggtaccggcc ttcaggctgt ggttgtcgaa cagcggcgca tcggtgcgcc catcgtggtt
    51181 gagcaccagg ctcttgagca gggtggcctg gtcgccctgg gtggcataca gcgcaacggc
    51241 catgcccgca gccgggcaac catgcatggt gtctagaacg tgggtgctga ggcccatggt
    51301 gtgtctcctg aaaacattga cagcgccaag cgtggcgccg tcaaagtgta tacaactttt
    51361 gtacgctgtt gccaggtttc gcttgggcgt ttaccctgcg gcccttcaca tccaaggaga
    51421 catcgatgca gcttcgcccc accctactgg ctgccagcct ggcgctggct cttttcggcc
    51481 tggccggctg cggcagcacg ccgcagcagc catcggcagc cgccacggcc cagcccggcg
    51541 cagcgcaggc tgccgctgcg gcgtatgact tcgacatgga catcttccat ccggtggtcg
    51601 ccagcaacgg catggtggcc agcgagcagg aattggcctc gcgcatcggc ctggacatcc
    51661 tgaagagcgg cggcaacgcc gtcgatgccg ccgtggccat gggctttgcg ctggccgtgg
    51721 ccctgccgaa cgccggcaac atcggcggcg gcggtttcat gatggtgcac gacgcgaaga
    51781 cgggcaaaag cgtggcgctg gacttccgcg aggtcgcgcc cgccaaggcc acgcgcgaca
    51841 tgtacctgga cgcccagggc aaggtgatcg atggcaagtc gctctacacc cactacgccg
    51901 tgggcgtgcc cggcacggtg gccggcatgg agcatgcctt gaagcgctgg ggctcgctgc
    51961 cgctgtcgcg ggtggtggcg ccggccgtgg cgctggcgga caagggcttt cccgtcagcg
    52021 agaccctggc caagatcctg cagcaggaac agaagaacat gggccgctgg cccgccaccc
    52081 aggccatctt ctggaagaac ggcgcgccac tcaagcgcgg tgatgccctg gtgcaaaaag
    52141 acctggcgca gtcgctgcgg ctgattggcg agcagggcgc caaggccttc tacgagggcg
    52201 ccatcgccca gaaaatagcc gccgagatgg ccccgcacgc gggcgccctg tcgctgcagg
    52261 atctgaagaa ctacaaggtg gtggagcgcg agcccgtgcg cggcagctac cgcggctacc
    52321 agatcgtgac catgccgccg ccgtcatctg gcggcgcgca cctgatccag atcctgaaca
    52381 tgatggagcg ctggcccatg aaccaatggg gcgtgaacag cgcgaaaagc atgcaccaca
    52441 tggccgaggg catgaagctg gcctatgccg accgcgccga atacctgggc gacccggact
    52501 tcgtgaaggt gccgctcaag ggcctgatct ccaagcgcta cgccgatcag ctggccgccg
    52561 gcatcaacgc cacgcaggcg cgcagcgcaa aggacatcaa gcccggcaag ccccagccgt
    52621 atgagagcga ccagaccacg cattactcgg tggtggacaa ggccggcaac gccgtggccg
    52681 tgacctatac gctgaacacc aacttcggca gcggcatcgt cgccaagggc acgggcatca
    52741 tgctgaacaa cgagatggac gacttctcgg ccaagcccgg cgtggccaac gcctatggcc
    52801 tggtgggcgg cgaggccaac gccgtggccg ccggcaagcg cccgctgtcg tcgatgacgc
    52861 ccacgctggt gctcaaggac ggcaagccca ccctggtcac cggcagcccc ggcggcgcgc
    52921 gcatcatcac cacggtgctg cagacggtgg tcaacaccat tgacttcggc atgaaccccg
    52981 ccgaggccgc cgccacgccg cgctttcacc accaatggac gcccgacgag ctgcgcatag
    53041 aaaagggctt ctccaacgac acgctggccc tgctcaagca atgggggcac aacgtggcgc
    53101 tgaaaccctc gatgggccgc acgcagacca tacagatacg cgacggcatg ctgtatggcg
    53161 cgtccgaccc gcgcaacccg gacgggcaga cgctgggcta ttgaggcgag gcgcgcccag
    53221 cacaaaggcc gcacccaccg ggcgcggcct ttttttgcgt cagttgggaa tcaggccggc
    53281 gccttctggc cgacctcgaa attcgccatc ttctccaaag cgcgcaccat gccggaatgg
    53341 tcccaggccg cgccgccatg ggccacgcag ctgttgaaca gctcctgcgc ctgggccgtg
    53401 ttgggcagcg agacgccaag ttggcgcgcg ctggacaggg ccaggttcaa gtccttttga
    53461 tgcagggcga tgcggaagcc cggatcaaag gtgcgcttga tcatgcgctc ggcatgcacc
    53521 tccaggatct tggacgaggc aaagccgccc agcagggcct cgcgcacgcg cgccgggtcg
    53581 gcgccggcgc gggacgcaaa cagcagcgcc tcggccacgg cctcgatgtt cagggcgacg
    53641 atgatctggt tggccacctt ggcggtctgg ccgtcgccat tgccgccgac cagggtgatg
    53701 ttcttgccca tcttctcgaa cagcggcttg acgcgctcga acacggcatc gggcccgcca
    53761 accatgatgg acagggtggc gttcttggcg cccacctcgc cgcccgagac cggggcgtcc
    53821 aggtactgcg cgcccacggc ctcgatgcgc ttggcgaaat ccttggtggc cacgggggag
    53881 atggaggaca tgtccaccac caccttggcc gcgctgccct tcaggcccgc cgccacgcca
    53941 tcctcggcga acagcacggc ctccacgtca ggggtgtcgg gcagcatcag aaagacgatg
    54001 tcggcgcgct cggccacacc gcgcgccgtg gtgcattgcg tggcgctgct ctcggccagc
    54061 gccgcgggca gcttgctgcg ggtgtggatg aacagctgat ggccggcggc gatcaggtgg
    54121 ccacacatgg gcgcgcccat gatgcccaga ccgatgaaac cgagcttgag gggggatgag
    54181 gtcatgtggt tttctccttg tctcttggaa taatcaaaat ttgtagctgc ttgcgcttat
    54241 caggcgggcg ctagagcctt ttttcctgcc aatttttcca gccaccccag gccggcctcg
    54301 gtaccctggg cgggcttgta ctcgcagccc acccagccgg cgtaaccgat gcggtcgagg
    54361 tgcgcgaaca ggaagggata gttgatctcg ccgctgccgg gctcgttgcg gccggggttg
    54421 tcggccagct gcacatgggc gatgcgcgcc aggttcttct gcagcgtggc ggccagctcg
    54481 ccctccacgc gctgggcgtg gtagatgtcg tactgcacga aggcgttgtc ggcgcccacc
    54541 tcgtcgagga tggcaatcgc ctgatccgtg cggttcaggt agaagcccgg aatgtcgaag
    54601 gggttgatgg gctcgatcag caggcgcagc ttggccatct tcagcgcggc ggcggcaaag
    54661 cgcaggttct cgacaaaggt acggcgcagc acggcggcgt ccacgccggc cggggccttg
    54721 ccggccaggc agttgagctg cggcacgccc agggcgtggg catagctcac ggccttggcc
    54781 acgccggcac ggaattcatc cacgcgctgc gggtcgcagg cgatgccgcg ctcgccggca
    54841 tcccagtcgc ccgcgggcag gttgtgcagc acgatctgca ggccggtggc atccaggcgc
    54901 tccttgatct cctcgaccgt gtgggcgtag gggaagagga actccacggc ttcaaaaccg
    54961 gcctgggcgg cgcgctcgaa gcgatcgagg aagggcacct cggtgaacag catgctgagg
    55021 ttggcggcaa aacggggcat atcgggtctc cagtcaaaaa aatacgtcaa tcgagcatgg
    55081 ccagcgcgct gggcacgtcg gcgccgctca gggccagctc ctcgaactcg accacattgt
    55141 cgatctcggc acccatggag atattcgtca cccgttccag gatgcattcg atgaccacgg
    55201 gcgtctggtg ctcggccatc caggcctcgg cctgggtcat ggcggcggca aagtcctcgg
    55261 ggcggtgcac gcgaatcgcc ttgcagccca ggccctcgac caccttcaca tggtccacgc
    55321 cgtagccgcg cgcggcctcg ccctcttcca tgttgatatt gtcaaacgcc agctgcacgc
    55381 agtagtccat ggagaaggcg cgctgcgact ggcggatcag gcccagatag ctgttgttga
    55441 ccagcacatg cacatagggc agcttgaact gcgcgcccac ggccagctcc tcgatcatga
    55501 actggaagtc gtagtcgccc gacagggcca cgatcttgcg cgtggggtcg gccacgcgca
    55561 cgcccagggc ggcgggcgtg gtccagccca gcgggccggc ctggccgcag ttgatccagt
    55621 ggcgtggctg gtagacatgc agaaactgcg cgccggcaat ctgcgacagg cctatggtgg
    55681 agacataggt cgtgtccttg ggcaggttgc ggttcatgca ctggtagacg cggtgcggct
    55741 tcatcggcac gttgtcgtag tgggtcttgc gcaggtagtc cacgctgttc ttgcggccct
    55801 ggcattcggc cacccagcta ctccgatcct tgagcctgcc tgcggcctgc cattccctgg
    55861 ccacggcaac gaactgctcc agggcggcct tggcgtcgga gacgatgcca tagtcgggcg
    55921 cgaacacgcg gccgatctgc gtcggctcga tgtccacatg gatgaacttg cgccccttgg
    55981 tgtagacatc gaccgaaccc gtgtggcgat tcgcccagcg gttgccaatg ccgaacacga
    56041 agtccgacga cagcatggtc gcattgccat agcggtggct ggtctgcagg ccgcacatac
    56101 cggccatcag cgggtggtcg tcggggatgc tgccccaacc catcagcgtg gggatcaccg
    56161 gcacattcag gatttcggcc agctcgacca gcagctcgga tgcatcggcg ttgatcacgc
    56221 cgccgccgga aacgagcagc gggcgctcgg actcgttgag catcgctatg gccttttcgg
    56281 cctgcttgcg cgatgcggcc ggcttgtagg cgggcagcgg ctcgtaggtg tcgatgtcga
    56341 attcgatctc ggccagctgc acgtcgatgg gcaggtcgat cagcaccggg ccggggcggc
    56401 ccgagcgcat caaatgaaag gcctgctgga aggcgcgcgg cacctgggcc ggctccagca
    56461 ccgtggtggc ccacttggtg accggcttgg cgatcgtggc gatgtccacc gcctggaagt
    56521 cctccttgtg caggcgggca cgcggcgcct ggccggtgat gcacaggatg ggaatcgagt
    56581 ccgcgctggc cgagtacagg ccggtgatca tgtcggtgcc ggccgggccg ctggtgccta
    56641 tgcagacgcc gatgttgccg gccacggcac gggtatagcc ctcggccatg tggctggcgc
    56701 cttccacatg ccgcgccagg atatggccta tgccgccatg ctccttcatc gccgcgtaca
    56761 acggattgat ggcggcaccg ggcacgccga acgtgacgct gacaccctct ttttcgagca
    56821 ccttgacggc ggcttcgatc gctttcattt tggccatggc ctatctcctt gaagtgggtg
    56881 caactttagg caaaccggca ccatggcgga aggccacggg agaccataga atttattccg
    56941 cgttggaata aacaaggaag atgcaatgga cagactggac gccatgcaca tgtttgtgcg
    57001 ggtggtggag accggcagct ttacccaggt ggcacgcgaa ttcgccacca cccagcccac
    57061 cgtgaccaag caggtcgcgg ccatggaggc gcggctcaag gtgcgccttt tgaaccgcaa
    57121 cacgcgcggc gtgagcctga ccgagccggg cacgctgtac tacgagaaat gcaaggccat
    57181 cgtgagcgac gtggccgagg ccgagaacgt ggtccaggtg cgccagcacc aggtgcaggg
    57241 ccagctgcgc ataggcagct cggtggcctt cgggcggcgc gtggtgatcc cgctggcgct
    57301 cgacttcatg cagcacaacc cccgggtcga ggtggacctg agcttcgagg accgctacac
    57361 cgacctggtg gccaacggca tagacgtggc gctgcgcctg ggcaaactgg ccgactccac
    57421 gctgggcgca cgcaccctgg gcgtgaaccc ctgggtgctg gtggcctcgc cccactacct
    57481 gaagaagcac ggcacgccgc gccgcccctc ggatctgaag gagcacgcca cgctgatcta
    57541 cagcagcgtg cagggcaatg acgtctggcg cctgcgcaat gccagcggcg aggcggtatc
    57601 cgtgcccgtc agcggacgac tgcgctcgaa caatctctcg gccctgctgg ccgcggcacg
    57661 cgcgcacatg ggcatcgccg ccctgccctg gtatgtggcc catgactcgc tggcggcggg
    57721 acgcgtggtc gaggtgctca agacctgccg cctgcccgag caggagattc acgccgtcta
    57781 cccctcgcca cgcctggtgc cgcagaaggt gcaggccttc atcgccttcc tgcagggacg
    57841 cttcggtagc acctggtggg aacaactacc caagggcgga tgacacctgg gactacacaa
    57901 gcgcgcccga ctcgttctcc taccagtcca ctgcgttgta agtcttgcta caggcttgct
    57961 tcctagaatc cgcgcgtcaa tgctgcactg cacaatttcg tgcgcagccg atgccattga
    58021 tggagtcagg tttatgtcca accccttgta tgaaaacatc aagcgcgacc cgcgctacgc
    58081 gcagttggtg cgcgagcggg cgcgctttgc cacgacgctg gccttgctgg tgattgccgt
    58141 gttctttggc tttgtgctgc tggtggcctt ccagccgcaa gtgattgcgc tgcgcctgtt
    58201 cgagggcagc aatctgacgc tgggcattgc cctgggcttt acccagtttg tgttcttctg
    58261 cgccctgacc tgggtctatg tgcgccgcgc caacagcgac tttgatgcgc gcaacgccca
    58321 gatcgtcgcc gacgccatga ggaagacagc atgatgcagc gccctatttc tctcgcctgc
    58381 cgcagcgctg cggccctgtc cctgctgctg ccggcactgg ccatcgccgc cccagacctg
    58441 ggcgccacac aaaagcagcc gctgaactgg cacgccattg gcatgttctt cgtcttcgtg
    58501 ctgatgacgc tgggcatcac ctactgggcg gccggccgct ccaagtcggc gtccgacttc
    58561 tacaccgccg gcggcggcat caccggcaag caaaacggcc tggcgattgc cggcgacttc
    58621 atgtcggcgg ccacgctgct gggcctgacg gccatgatct atggcgacgg tgtcgatggc
    58681 tacatctaca tggtggcctt cttcgtcggc tggcccatca ttttgttctt gatggccgag
    58741 cgcctgcgca acctgggcaa gttcaccttt gccgacatca ccgcctaccg cctctcccag
    58801 ggcccggtgc gcaccatggc ggccgtcagc tcgctgaccg tggtctgctt ttacctggtg
    58861 gcgcagatgg tcggcgccgg ccagctcatc aagctgctgt tcggcctgga ctacaacgtg
    58921 gccctggtcg tcgtcggcgt gctgatgatg gtgtatgtga tcttcggcgg catggtcgcc
    58981 accacctggg tgcagatcat caaggcctgc atgctgctgg cgggcggcac ggccatcatg
    59041 ctgctggcct tctcccagtt cggcttcagc ctggacaccc tgctgaccaa gaccatggag
    59101 gtgcacaagc tgggcgccaa catgctctcg cccggcaagc tgctggccga cccggtgacg
    59161 gccctgtcca tgggcctggg cctgatgttc ggcatcgccg gcctgccgca tatcctgatg
    59221 cgcttcttca ccgtgaccaa cgcgcaggag gcgcgcaagt cggtgttcta tgcctccggc
    59281 atcatcgcct tcttcttcaa cgtgatcgcc atcatgggcc tgtgcgccat cgtgctggtg
    59341 ggcaccaatc ccgacttctt cgagggcggc gtggtcggcg gcaagctgat cggcggcggc
    59401 aacatggtgg ccctgcacct ggccaaggcc gcgggcggca acctgctgct gggctttttg
    59461 tcggccgtgg cctttgccac catcctggcg gtggtggcgg gcctggcgct ggcaggtgcc
    59521 tcggccatct cgcacgacct gtatgcccgc gtgatcatga agggccaggc gagcgaggcc
    59581 aaggaaatcc gcgtctccaa gatggccacc ctgggcctgg gcgtggtcgc cgtgctgctg
    59641 ggcatggcgt tcgagaagat gaacgtggcc ttcatggtgg cgctggcctt tggcgtggcc
    59701 tcctgcgcca acttccccgt cctgctgatg tccatgtact ggaagggcct gaccacgcgc
    59761 ggcgcgctgg tgggcggcta cacgggtctc atcagctcgg tgctgttcgt gctgttctcc
    59821 aagtcggtgt gggtggatgt gctgggcaac aaggccgcgc tcttccccta cacccagccg
    59881 gcgctgtttg ccatgccgct ggccttcctg gcgatctatg tcttctccag gctggacagc
    59941 agcgcccagg ccgacaagga gcgtgcggca tttgatgacc agtacgtgcg cgcgcagacc
    60001 ggcgtgggcg ccgccagcgc cgctgcacac tgatgcagca gcccggggac ggcaagcgtc
    60061 cctgggcgcc tacaacacca ggatggcgcg aaagtcgttc acattggtgt tcgtaggccc
    60121 ggtgaacacc agatcgccca gaggttcgaa aaagccatag gcatcgttgc gatccaggtg
    60181 gtcgccgatg cgctggccgt tttgtgccgc gcgcgccagg gtgtcgggtg ccacgcgggc
    60241 gcccgcgttg tcctccacgc cgtctatgcc gtcggtgtcg gccgccagcg cccagacacc
    60301 ctcctgcccc tgcagcgcct gggccaggcc caggcagaac tcgccggcac gcccgccgcg
    60361 ccccttggcg gcgccgggct gcctggggcg tatcgtgacc gtggtttccc cgcccgaaag
    60421 aatcacgcag ggccgcgcaa acggctcatc gcgccgggcc acggcgcgcg ccagcgccgc
    60481 atgcaccttg cccacctcgc gcgactcgcc ctcgatctcg tcggacagga tatgcgccgc
    60541 aatgcccgcg gcctgcgccg cccgggccgc ggcctgcagt gactgctggg gcgtggcgat
    60601 caggtgcacc tggtgaccgg caaagcgcgc gtcaccgggc ttcggggttt ccagcgcgcc
    60661 agcctgcagc gcggcgcgca cggcatcggg cacggcaatt tggtagcgct gcagaattgc
    60721 cagcgcgtcg gcgcaggttg aggcatcggc caccgtgggc ccgctggcga tgatggaggg
    60781 gtcgtcgccc ggcacgtcgc tgatggtcag cgtgaccacc cgggccgggt ggcaggcggc
    60841 ggccaggcgg ccgcccttga tgcgcgagag atgcttgcgc acacagttca tctcgccaat
    60901 ggcggcgccg ctttccagca aggcctggtt gatgcgctgc ttctctgcca gatccagacc
    60961 ctcggccggc agggtcaaga gggccgagcc gccgccagag atcaggcaca gcaccagatc
    61021 gtcctcggtc agcccttgcg tcagcgccag catgcgctcg gcagccagca agccggcggc
    61081 atccggcacg gggtgagcgg cctcaacgag ttcgatacgc tgcgccagcc cctcggggcg
    61141 cggcggaata tggtggtagc gcgtaaccac caggccggac agcggcgcct cctgcggcca
    61201 cagcgcttca agcgcctggc acatcgaacc gccggccttg ccggcgccca gcaccacggt
    61261 acggcccttg ggaggcggcg gtagatgctg acccatgctg tgcagaggtt gggcgcgctg
    61321 caccgccacg cggtacagat cgagcaagaa ggcctgggga tcctgcaggg gccgggacgg
    61381 ggttgtggtc atgcgcgctg tctcctgtat gagtgcattg ccgggttcaa ggttgaatgg
    61441 tggcgtactt tcccttattc gtccacaaaa actgtataca actcctgtcg ccaaaggcta
    61501 tgatggcgtg catggaaacc tccaccacca gtttcatcgt cgagaacctg acccgcgcca
    61561 tcgtggagca ccgcctgttg cccggcacca agctggccga gcaaaagctg gcggatcact
    61621 ttggcgtctc gcgcacgctg gtgcgccagg ccttgttcca actgtcgcaa aaccggctca
    61681 tcaagctgga gccggcgcgc ggcgcattcg tggccacgcc ctcggtggct gaggcgcgcc
    61741 aggtattcgc cgtgcgccgc atgctggagg ccgagatggt gcgcgccttc atggcccaga
    61801 gcacgccggc caagatccgc agcctgcgcc agcatgtggc cgccgagaaa aaggccatgg
    61861 agcgcgagga cgtaggccag cgcaccgagc tgctgggcga ctttcatgtg cgcatggccg
    61921 agctcatggg caacgaggtg ctggcccagc tgctgggcga actgatctca cgctgcgccc
    61981 tgatcacgct gatgtaccag tccaacgtgg ccgccgagca ttcgcacgac gagcatgccg
    62041 acatcgtcga ggcgctggcc ggcggcgacg ccgaccatgc cgtgcagctc atgcgccagc
    62101 acctggacaa cgtcgaggcg ggcctgacct tcgaccgcga cgttccgacc agcgaccttt
    62161 ccatggccct ttctgcctga gaactgcagc acgctccaac accatgacct acgacgccac
    62221 tgccccctac ccccgcgacc tgatcggcta cggccgcacg cccccgcagc cccagtggcc
    62281 gggccaggcg cgcgtggccg tgcagtttgt cctcaactac gaggaaggcg gcgagaactg
    62341 cgtgctgcat ggcgacgccg ccagcgagca gtttttgtcc gaaatgttca acccggccgc
    62401 ctaccccgag cgccacatga gcatggaggg catctacgaa tacggctcgc gtgccggcgt
    62461 gtggcgcatc ctgcgcgagt tcgagcgccg cggcctgccg ctcaccgtct ttggcgtcgc
    62521 cacggcgctg caaagacacc gcgatgtgac ggccgcgttc accgagctgg gccacgagat
    62581 cgcctgccat ggcctcaaat ggatccacta ccagggcgtg cccgaggagg tggagcgcgc
    62641 ccacatggcg gaggccatgc agatcatcca gggtctgacc ggcagccgcc cgctgggctg
    62701 gtacacgggc cgcgacagcc cgcgcacgcg ccgcctggtg gccgaccatg gcggcttcga
    62761 gtacgacagc gactactacg gcgacgacct gccgttctgg atgaaggtcc agaagaccga
    62821 cggcagcgcc gcgcaccagc tcatcgtgcc ctacacgctg gacgtgaacg acatgcgctt
    62881 tgcgctgccc cagggctact cgcacgccga cccgttcttt cagtacatga aggacagctt
    62941 cgacgccctg tatgccgaag gcgacccggc cggcgacaac gcgcccaaga tgatgagcat
    63001 aggcatgcac tgccgcctgc tgggcaagcc cggacgcatc accgcgctgc agcgctttct
    63061 ggaccatatc cagggccaca acaaggtctg gatctgccgg cgcatcgaca tcgcgcgcca
    63121 ctggaaagag cgctttcccg caccaatttt gtaaagaaaa caggctctag cgcttatcca
    63181 tcaaacgctg acagctatca ttttaatagg atacaccgtg gctttgaccc tggaacaact
    63241 gaaccgcgcg cccttgcccg aggccacgca aatgctcgac ggcctgtacg agcacacgcc
    63301 ctggatcgcc gagcaggcgc tggcgcagcg ccccttcacg tcgctcgccc atctcaagca
    63361 cgccatggtg cgggtgctgg ccgccgcccc gcgcgaggcg cagctggccc tggtgcgcgc
    63421 ccacccggag ctggcgggca aggccatggt cagcaagacg ctgacggcgg aatcgaccaa
    63481 cgagcaaagc aaggccggcc tgaccgactg cacgcccgaa gagttcgccc gcatccagca
    63541 gctcaacagc gactacaacg cgcgccacgg cttccccttc atcctggccg tgcgcggccc
    63601 gcgcggccta ggcttgccga agcaggaaat catcgacacc tttgcgcgcc ggctggagaa
    63661 cccggtcgat ttcgagctgg ccgaggcgct gcgcaacatc caccgcatcg tcgagatccg
    63721 cctgaacgac aagttcggct ttgcccccac gctgggcaac gatgtctggg actggcagga
    63781 aaaactcagc acgcacagcg acccgggcta cgccgagaag ggccagctga ccgtcaccta
    63841 cctgaccgac gcgcaccgcg cctgcgccca gcgcatcagc cactggatgc gcgattgcgg
    63901 cttcgatgag gtcgagatcg acgccgtggg caacgtggtg ggccgctacc acggcgccca
    63961 gcccgatgcc aaatacctgc tcaccggatc gcactacgac accgtgagga acggcggcaa
    64021 gtacgacggg cgcctcggca tcttcgtgcc catggcctgc gtgcgcgagc tgcaccgcgc
    64081 cggccgccgc ctgcccttcg gcatcgaggt ggtgggcttt gccgaggaag aaggccagcg
    64141 ctacaaggcc accttcctgg gctcgggcgc gctggttggc gacttccgcg ccgaatggct
    64201 ggagcagaag gacgccgacg gcatcaccat gcgccaggcc atgcagcacg ccggcctgtg
    64261 cgtggacgac atccccaagc tgaagcgcga ggccgccaac tacctgggtt ttgtcgaggt
    64321 gcatatcgag cagggcccgg tgctcaacga gctggacatc ccgctgggcg tggtcacctc
    64381 catcaacggc agcgtgcgct tcgtcggcga ggtgtgcggc atggccagcc acgccggcac
    64441 cacgcccatg gatcggcgcc gcgacgccgc cgccgccgtg gccgaattga ttctctacgt
    64501 ggaaaagcgc gccgcccagg acggcgacag cgtcggcacc gtgggccagc tggaggtgcc
    64561 cagcggctcc atcaacgtgg tgccggggcg ctgccgcttc agcctggatc tgcgcgcacc
    64621 caccaacgcg cagcgcgacc gcctggtgca cgacatagag gccgagctgc aggccatctg
    64681 cgcgcgccgc ttcgtgcatt acagcctgga ggaaaccatg cgcgcggccg ccgcgcccag
    64741 cgcgcccgag tggcagcagc gctgggaaaa ggccgtcgcc gccctgggcg tgccgctgta
    64801 tcgcatgccc agcggcgccg gccatgacgc catgaagctg cacgagctca tgccccaggc
    64861 catgctgttc acgcgcggca tcaacagcgg catcagccac aacccgctgg aggcgacgac
    64921 cagcgacgac atgcagctgg ccgtcgatgc cttcacccat gtcttgaacc aactggccca
    64981 agaggcctga agaaagcagt ccccatgagc aactaccaac aactggacgc ctggatagac
    65041 cagcatttcg acgagcaggt gcagttcctg caggccctgg tgcgcgtgcc caccgacacg
    65101 ccaccgggca acaacgcgcc gcacgccgaa cgcaccgccg aactcatcaa agactacggc
    65161 ttcgaggccg agaaacatgc cgtgccccag gccgaggtgc aggcctatgg catggagtcg
    65221 atcaccaatc tcatcgtgcg ccgcccctac ggcaacggtg gccgcaccgt ggcgctgaac
    65281 gcccatggcg acgtggtgcc cccgggcgag ggctggagcc acgaccccta cggcgccgag
    65341 attgtcgatg gcgccatgta cggccgcgcc accgccgtga gcaagagcga cttctccacc
    65401 ttcaccttcg ccgtgcgcgc gctcgaggcc gtggccaggc ccaccaaggg cacgatcgag
    65461 ctgcacttca cctacgacga ggagtttggc ggcctgctcg gccccggctg gctgctggag
    65521 caaggcctga ccaagccgga cctgatgatc gccgcaggct tcagctacga ggtcgtcacc
    65581 gcacacaacg gctgcctgca gatggaagtc acggtgcacg gcaagatggc ccacgccgcg
    65641 gtgccgcaca cgggcgtcga cgcgctgcag ggcgccacgg ccatcatgaa tgcgctgtac
    65701 gccgagaacg tgaagtacca gcaggtcacc tcgaacatgc ccggcatcaa gcacccctac
    65761 ctgaacatcg gccgcatcga gggcggcacc aacaccaacg tgattcccgg caaggtggtg
    65821 ctgaagatag accgccgcat gatccccgag gaaaacccgg ccgaggtcga ggccagcatc
    65881 cacgccgtca tcgccaaggc ggtgcaggac ttcaaccagc agcgcgacta ccagggccag
    65941 gacgccgtgc gcaccgacat caggcgcatc ctgctggcca acgccatgac gccgctgccc
    66001 ggcaacgcgc cgctggtgga ggccatccag cagcatggcg aagcggtgtt tggcgaaaag
    66061 cccgccgccg tgggcacgcc gctgtacacc gacgtgcgcc tgtacgtgga gcgcggcatt
    66121 cccggcgtga tctacggcgc cggcccgcgc accgtgctgg agagccacgc caagcgggcc
    66181 gacgagcgcg tacagctgga agacctgcgc cgcgccacca aggtcgtggc acgggcgctg
    66241 catgacttga tggcctgatc agctatccat ccgcaatcac caagcccgct gctgcagcgg
    66301 gcttttttgt tgcaacaaac cgtgccctcc gcagctacag ccggggtttg aacgccggcc
    66361 tttcgggtta ctaccgaatc aacaatgctt atagggtgta tacacttttt ccatacgaaa
    66421 gcgcttgcca cctgacgcgc gcgtatgcaa gcacatccat cagcccttga gcccgcccat
    66481 attcctggag acagcccccc atgaccaccg cagtccaccc cgtcgatgag acattacccc
    66541 tgggtcgcct cacggccctg ggtttgcaac atgtgctggt gatgtacgcc ggcgccgtcg
    66601 ccgtgccgct gatcgtcgga cgcgcgctga acctgccgcc cgaacaggtg gcgcacctga
    66661 tctccgccga cctgttcgtc tgcggcctgg tgacactgat ccaggcctgg ggggccacgc
    66721 agtggtttgg catcaagctg ccggtgatga tgggcgtgac ctttgccgcc gtcgcgccca
    66781 tggtgtcgat ggcgcaggcc accggcggcc atgccggggc gggcctgatc tttggcgcgg
    66841 tgataggcgc gggcgtggtt tccatcctga tcgcgcccat gatgagccgc atgctgcgct
    66901 tctttccgcc ggtggtcacc ggcaccatca tcgcggtcat cggcatcagc ctgatgcgcg
    66961 tcggcatcaa ctggatcttt ggcaacccgg tcggccccac tgcgccctcc gtgcccaacc
    67021 cggaacacct gaaatggctg gccgaggccc aggccgccgc cggcgcgccg ggctcgtccc
    67081 tggctccggt gcccaagggc tttgccatcg tgcccaccat tcccaacccc aagtacgccg
    67141 acctgacggg cgtgggcatc tccggcatcg tgctgctcac catcttgctc atcgcgcgct
    67201 ttggcaaggg cttctgggcc aatatctcgg tgctgttggg cattgctgcg ggcggcgtca
    67261 tcactgccgc gatgggcctg atggactttc acaaggtggg caaggcgcag tggttcgaca
    67321 tcgtgctgcc gttccagatc gccatgccgg tcttcgaccc catcctgatc ctgaccatga
    67381 ccctggtgat gatcgtggtc atgatcgaat ccaccggcat gttcctggcc ctgggcgaga
    67441 tgaccgacaa ggaaatctcg caggaagacc tcaagcgcgg cctgcgcacc gatggcctgg
    67501 gcacactgat cggcggcatc ttcaacacct tcccctacac cagcttctcg cagaacgtgg
    67561 gcctggtggc ggtcaccggc gtcaagagcc gctgggtctg cgtggccggt ggcgtgatcc
    67621 tgatcattct cggcgtgctg cccaagatgg cggcgctggt cgaatccctg cccaccgtgg
    67681 tgctcggcgg cgccggcctg gtgatgttcg gcatggtcgc cgccaccggc atacgcatcc
    67741 tgtccaaggt ggacttccag cacaaccgca acaacgccat gatcgttgcc gtctccatcg
    67801 gcgtgggcat gatcccgctg gtggcgccca acttccgcca gtggatgccg cattccatcc
    67861 acccgctgat cgagtcaggc atcctgctgt cctcgctggc cgccgtggcg ctgaacgtgt
    67921 tcttcaatgg cgccaagcat gacgcccgcg ccgccatcga ggccgccaag caggccgagg
    67981 cgcactgacc gcccaacgcc agacccggcc cgcacagggc catgcagcgc ggggcccgga
    68041 gccccgcgca gcgcgtatga gccgaccgcc gcgtcgcgca ctatttaccc gtgaatcacc
    68101 gccgataccg gcgcgcttgc agtaagcttg ttcgccccgt attcgccgtc cctttgctac
    68161 tgattggatt tcgtatgaac cagccactgt ccgccgccga agtcgccttg cgcgaagccg
    68221 ccctggaata ccaccgcaac cccagcaagg gcaagatttc cgtcacccct accaagccgc
    68281 tgtccaacca gcgcgacctg tcgctggcct attcgcccgg cgtggcctat ccctgcctgg
    68341 acatccaggc cgacccgagc aaggcgtttg actacacctc gcgcggcaac ctggtgggcg
    68401 tgatcaccaa cggcacggcc gtgctgggcc tgggcgacat aggcccgctg gccggcaagc
    68461 cggtcatgga aggcaagggc tgtctgttca agaagttcgc cggcgtggat gtgttcgaca
    68521 tcgagctcga cgcacgcgac ccggacaaga tcatcgagat cgtcgccgcg ctggagccca
    68581 cgctgggcgg gatcaacctg gaggacatca aggcgccgga atgcttctac atcgagcagg
    68641 agctgtccaa gcgcatgaac atccccgtgt tccatgacga ccagcatggc acggccatca
    68701 tctccagcag cgccctgctc aacggcctgg agctggtcgg caagcagatc gaccaggtca
    68761 aggtagccgt ctcgggcgcc ggcgcggcgg ccattgcctg cgtggacgtg atggtgggcc
    68821 tgggtgtgaa gcgcgagcat gtcttcatgg tcgattccaa gggcgtgatc tacgagggcc
    68881 gccccggtgg catggatgcc tccaaggcgc gctacgcgca aaagaccgag gcgcgcaccc
    68941 tggccgatgt ggtcaacggc gccgatgtat tcctgggctg ctcggccccc ggcgtgctca
    69001 ccgccgagat ggtcaagacc atggccgaca agcccatcat cctggcactg gccaatcccg
    69061 agcccgagat ccgtcccgag ctggccaagg ccgtgcgccc cgactgcatc atcgccaccg
    69121 gccgctcgga ctaccccaac caggtcaaca acgtcctgtg cttcccctac atcttccgcg
    69181 gcgcactgga ctgcggcgcg accaagatca ccgaggccat gaagctcgcc tgcgtgcgcc
    69241 agatcgccgc gctggccaag gaaaacatca gcgaagaagt cgccaacgcc tacgccggca
    69301 aggagctggc cttcggcccc gactacctga tccccacgcc attcgactcg cgcctgatcc
    69361 tgaagatcgc ccccgccgtg gcccaggcgg cgctcgagtc cggtgtggcc tcccgcccca
    69421 tcaccgacat ggatgcctac aggagcagcc tgacgcgctt tgtctaccag accggcatgc
    69481 tgatgcgccc ggtgttcaat gccgccaagt ccctgcccaa ggcacaaaaa cgcgttgcct
    69541 acgccgatgg cgaggacgag cgcgcgctgc gcgccgccca gttcgccatt gacgacaacg
    69601 tggcccaccc catcctcatc ggtcgcccgg cggtgattgc cgcgcgcatc gccaaggccg
    69661 gcctgcgcat ggagcctggc aaggatgtgg aagtgtgcga cccctcggac gacccgcgct
    69721 ttcgccagta ctgggaaacc tactaccagc tcatgaagcg cgacggcgcc acgcccgaga
    69781 tggccaaggc cgccgtgcgc cgctccaaca ccatcatcgc ctcgctgatg gtgaagctgg
    69841 gcgacgccga tgccatgatc tgcggcctgg tcggcaccta cgagacgcat ctggagcgca
    69901 tccaccacat cctgggccac tccccggacg ccaaacaata cgcggcgctg aacgcgctga
    69961 tgactcccaa caacggaaca ctgttcatca ccgacaccta cgtcaacgaa gaccccaccg
    70021 ccgaggagct ggccgacatc gcctgggccg ccgtgcagga ggtgcagcgc tttggcatcc
    70081 cgccgaaggt ggcattcctg tcgcactcca gctttggctc gtccaagcgt gcctcggcac
    70141 gcaagatgcg cgccgcgcgc gacctgttcg tggcgcgcca tcccgacatc gaatgcgatg
    70201 gcgaactgca cggcgacgcg gcgctggagc ccaaggtgcg caacacctac ttgcaggatt
    70261 ccacgctcac cgccccagcg aacctgctgg tctgcccgaa catcgacgcg gccaacattc
    70321 tctacaacgt actcaaaacc acgaccagcg gtggcgtgac cgtgggcccc gtgctcatgg
    70381 gcacctcagc gtcggcccat atcctcacgc ccgctgccac cgtgcgccgc gtgctgaaca
    70441 tgaccgccct ggccgtggtc aacagcgcca cgcgcaatca gtaagctcca ttactgacaa
    70501 caaaaagggc tggcgccact cgcggcgcca gccctttttt tcaggcggtc tcaatcccgt
    70561 acttactgct tgatggcctc gatctgcgcc acgatgcgca cattcttcgg aaaaccgtac
    70621 tgcacgccat agtccgcgcc aaaggccgtg cgatctatcg tggtttcaaa gtcgccaccg
    70681 caaacctcgc gcttgagcat ggggctgtcg tagcaggcga actgattggc cttgaaggtc
    70741 acgggctggg tcttgccctt gatcgtcagg ttgcccgaga cagccgtgag cttgtcgccc
    70801 tcataggtga acttgtcacc gacgaactgc gccgtgggga acttggcggc atcaaaaatt
    70861 tcggcactct gcaggtgttt gtcgaactgc gccgtgccgg tgttgatgga gttgatgtgg
    70921 aacgtcagct ccaccttgcc cacccggcca gccttgtcga actgcaccga accctccttc
    70981 ttgtcgaagc ggccgcggtt cacgctggcg ccaaagtgcc cgatctcaaa ggtggcaaag
    71041 gtgtgcgtcg ggtcaatggc ataggtcgca ggctcggcat gcacggcggt ggcggtcaat
    71101 gcagcagcgg caatggtgag gaaggcagaa cgcatgaatc ggtactccag agttgtttga
    71161 acagtcgtgt tgagaaaaag tcgcaatcaa agtttcggca cgccggtcaa ggccagcttg
    71221 aatttgacct ggacgtcatc ggccaccatg gacgtatccg cccattcgtt ttcgccaatc
    71281 ttgaaagcca ggcgcttgat gacaaagctg ccggcggccg tggtgttggc accggcctgg
    71341 gtcaatgtga cgggcaccag cacatcatgc accgccccct tgatgctgag cttgcccgcc
    71401 acctcgtacc tgccagcgcc caccgccttg atggcagtgg actcgaaggt ggcctgaggg
    71461 aacttgggca cattgaacca gacggccttc ggcagttcgg catccgtctc acgcacaccc
    71521 aatgtggcgc tactggtatc caccgtgaaa gccaccttgc tcgttgccgg cttggcaggg
    71581 tcgaacgcca cctgtgcgtc gaatttcttg aaccggccct cgacgggaac gcccatttgc
    71641 ttgctgacaa aggcgatttc gctgttctcg gctatcagtt tttgctgagc gagagcgggg
    71701 ccagtggcca ggcaggccgc gccagccagg gcccaggcca tgtgtcggga aaaaaccatg
    71761 gttgtcattc aaaaatcctg tgtgcgttat tggatgaagg cgggcatcaa ccgcggccag
    71821 gcagcatgcg tgccatcaag ccgtcacggt caatgaagtg atgcttcacg gccgccgcca
    71881 catggagcac caccagcaac gccaatgcat aggcagccaa cccatgcaag ggcttgaggg
    71941 catctgccaa ttcagggctg acgggcacaa agtcgggcaa cggcagccag cccagaaaca
    72001 caatcggaaa acccgccgcg gagctatatg cccaacccag caaggggacg atgaagaaca
    72061 agccgtacat caggtggtgc gtaccgtgtt gtgcccgctg ctgccagcgc ggcatggccg
    72121 cctgcatgtc tgcgggcagc gcgggcggtc tgcgaatgaa acgccacacc aggcgcagca
    72181 ccgacaaggc cagcaccgcc atgcccgccc acttgtgcca gttgtaaagc ttgaggcgct
    72241 gcggcgaaaa cggcagcccg gtcatgtacc agcccgcgac cagcatgacc acgatggcca
    72301 gccccagcac ccaatgcagg gcgatagcag gggcaccata gcgtgcgggg cgttgcgtag
    72361 taacagggct catggaatat gcgtacaaga aactggatcg tcggtatttt gtgccattgc
    72421 cccttcaagg gttcaaaaat caatgactta tgaagtttag ggggcgatca atgcgcctag
    72481 tttcaatcga attgttgttt tagttcaaat tttatgaaca tccagggctc ccatgttgcg
    72541 gcggcgcacc agatcaacgc atggccaccc aaagattgcc tcggcttgat tacactctga
    72601 tacagcgatg aatcccccaa acacccccgt cgttttcggc accgagaaca tcctgctgtc
    72661 cctgtgcaac tccgtcacgc gtgtgctttc cgtggccacg caagggccgg tgcactactc
    72721 ggccatggtg cagcgcatca ccaagacctg cctcaaaccg gacattggct gctttgtgct
    72781 gttcgatggc ggcttctccg ggctggtcat catcaacttt tcggccgccg ctgccatgga
    72841 gatctacgag cgctacatgc tcagcatggg aatggcacgc gaagaactgg ccagctcctt
    72901 cacttcggac gaggtgagca acgtcatggg cgagctgatg aaccaggtgg tgggcgactt
    72961 cacgggcaag gtgcggcgcg agctgcagac ccacatcaca caaaaccagc ccaagatgct
    73021 ggtgatcaac cagcaggtga tgctgagcgt ggacgccaat ctggatcagc ccgaggcacg
    73081 ccgcgtctcc ttctacaccg ccaacaacaa catcttctac ctggaactgg cgatagaccg
    73141 cacgcagttc atcaagctct acgatttcga gccccaggat gcgcccgacc ccgacgccct
    73201 gatggcccag gccgccgggg ctgcggcaac ggccacggca gcccttcccg atccggacgc
    73261 agacaccgag gcattgctca aatccctggg tatgtagatc gccggcggcc gggcagcgcc
    73321 gctcgcgcca gcggccaatg ccctcccgcg ggcatgcata tcgggcacga caaggggttt
    73381 cagggtaaaa tcgcctgttg cggtcggggg caccatgcct agagtccacc accccactcc
    73441 cccggctttt cgtcgacttt tgtcgactaa tacctgtgag atagaagtgc catggcgatc
    73501 gttgtcaaca agccccttcc cgaatttgaa gccaatgcga ccggcggaat caaggtttcc
    73561 aatgtatcgc accagggcca gatcctgatt ctgtacttct accccaagga caatacaccg
    73621 ggttgcacca ccgaggccat gcaattccgt gacaagttca aggactttga aaaagccggt
    73681 gccgtggtat ttggcgtgtc gcgcgacaac atgaagtcgc acgacgactt caaggagaag
    73741 ctggagctgc ccttcgagct cattgccgac accgaagaga agatgtgcca catgttcggc
    73801 gtggtcaaga acaagatcat gtacggcaag aaggtcaagg gcatagagcg cagcaccttc
    73861 ctcatcaatc ccgagggcat cctggtgcag gaatggcgcg gcctgaaggt gcccggccat
    73921 gtggacgagg tactcaaggc cgtgaaggcc atcaaggcac ccgtcaagaa ggccgcgtaa
    73981 acccggtacg acacgacccg gagccgtcac atggggcatg cataatgaat tcatgcggct
    74041 gaaaaacgct acctgcccct ctccacacaa agccgcctcg gtctccaggc ggctttgtgt
    74101 tttttgaatg cccctccagc gaggcctttc acaccatgcc cctgcccccc gccccgacca
    74161 agcgcgccgc cctgctctct cccgatgcct accgtctggc agcaccccag gaagaagcac
    74221 ccaacagcgc cgacacagag acattgccca gcgccacgcg caagcgcgcc accagcaaaa
    74281 acaaggccgg cagcgtggca ggcccagcca tcaccggcac cgccacggca acacctgcgg
    74341 tcgccgctgc cccggccaag ccggccatca ccggccccgc ccccgtcaca caggcacgcg
    74401 gccggcccgc agcggacaaa cccgccagcg atggcagtga cgccacgccc agcaacgtca
    74461 tgccgctgcg cgccgcaccc gccggcgcag cacgcaagcg ccgcgccacg ggcccgaaaa
    74521 agatgttcgt gctcgacacc aatgtgctgc tgcacgaccc gaccagcctg ttccgcttcg
    74581 aggagcacga catcttcctg ccgatgatcg tgctcgagga gctggacgcc cacaaaaagg
    74641 gcatgaccga ggtggcacgc aacggccgcc aggccagccg cacgctggac gcgctggccg
    74701 cagcccatgg cgcggccgac atggcgcatg gcctgaagct ggatgcgacg ggccagcgcg
    74761 ctgccacggg cagcctgttc tttcagacgg ccccgctgtc ctaccagctg ccggccagcc
    74821 tgccccaggg caaggccgac aaccagatcc tgggcgtggt cgaggccctg cgcaagctgc
    74881 acgcgccgcg cgaggtggtg ctggtgtcca aggacatcaa catgcgcgtc aaggcccgcg
    74941 ccctgggcct ggcggccgag gactaccaga acgacaagac gctggaggat ggcgacctgc
    75001 tgtactccgg cagcctggcc ctgccccagg acttctggag caaggccggc aagaacgtgg
    75061 agagctggca aagcggcgtg cacatgtact accgcatcgg cgggccggtg gtggcacagc
    75121 tgatgatcaa ccagttcgtc tacttcgagg caccgggcga gcccagcctg tacgcgcgcg
    75181 tgaccgagat ccgcgacaag acggcggtgc tggagacgct caaggactac ggctcggcca
    75241 agaacgccgt ctggggcgtg aacacgcgca accgcgagca gaacttcgcc atgaacctgc
    75301 tcatggatcc cgacgtggac ttcgtcaccc tgaccggcac ggccggcacg ggcaagacgc
    75361 tgatggccct ggccgccggc ctgacccagg tgctggacga gcgccgctat accgagatca
    75421 tcatgacccg cgcgacggtg agcgtgggcg aggacatagg ctttctgccc ggcaccgagg
    75481 aggaaaagat gggcccctgg atgggtgcgc tggacgacaa cctcgaattt ttggccaagg
    75541 gtgacggcgg caacgccggc gagtgggggc gcgcggcgac caacgagctg atccgaagcc
    75601 gcatcaagat caagagcatg aacttcatgc gcgggcgcac cttcctgaac aagtacgtca
    75661 tcatcgacga ggcgcagaac ctgacgccga agcagatgaa gaccctgatc acgcgcgccg
    75721 gccccggcac caagatcatc tgcatgggca atctggcgca gatcgacacg ccctacctga
    75781 ccgagggcag ctcgggcctg acctacgccg tggatcgctt caagggctgg ccgcacagcg
    75841 gccacatcct gctggcgcgt ggcgagcgct cgcgcctggc agactttgcc agcgaggttt
    75901 tgtaacgcga aacagctatc atatctatag cttcgtgcgc agcgcatatg cgcctgaagg
    75961 cgggttttga ttcaaaaccc gccttttttt tgcctcacac atcaaaacgc acgccctgcg
    76021 ccagcggcag cgcgccgctg tagttgatgg tgttgg
//
LOCUS       DAOMTQ010000002        70224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_14_length_70224_cov_27.459761, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000002 DAOMTQ010000000
VERSION     DAOMTQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 70224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70224
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14_length_70224_cov_27.459761"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..971
                     /gene="accD"
                     /locus_tag="PK518_00355"
     CDS             99..971
                     /gene="accD"
                     /locus_tag="PK518_00355"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRM47389.1"
                     /translation="MSWLEKLLPSKIQQTNATERRQMPEGLWTKCPSCETVLYKSDLE
                     QNQNVCPKCSHHHRIGARARLNAFLDGEGRYEIGQEVLPVDALKFKDSRKYPERLKEA
                     LENTGETDALIVMGGAVKSINLVAACFEFDFMGGSMGSVVGERFVRGVETAIEQKVPF
                     ICFTATGGARMQEGLLSLMQMAKTNAALTRLAKKGLPYISVLTDPTMGGVSAGFAFVG
                     DIVIAEPKALIGFAGPRVIESTVRVTLPEGFQRAEFLQTKGAVDFICDRRELRETVAQ
                     CLSMLQRLPADAVA"
     gene            complement(1032..1586)
                     /locus_tag="PK518_00360"
     CDS             complement(1032..1586)
                     /locus_tag="PK518_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRM47390.1"
                     /translation="MLFQIISYLLEVVSGLLTGACLLRLYMQLQRVPFGNPVGRLVFA
                     LTDWLVLPLRKLIPAAGRWDLSCLVAAFLLQLVQYLLLWLLVGAGLGLAWLPWMALFS
                     LARVTVSGLMGLLIAYAILSWMQTRTPISDVIARLSEPLLQPVRRVVPLLGGIDLSPL
                     VLLVLLQVLSIMLGHLQASVLMGQ"
     gene            1678..2313
                     /locus_tag="PK518_00365"
     CDS             1678..2313
                     /locus_tag="PK518_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRM47391.1"
                     /translation="MPDPLTLTSLPLFPLNTVLFPGGLLALRVFEVRYLDMVRKCRQT
                     GSPFGVVTLTAGQEVRSAAAPEERFQPQGTLAVIEQLQTPQPGLITLHCRGTERFHIT
                     QQHQLPYGLWLADVQQLPDDPQVPLPADLQSLAAKLGLLLAAQHQRRSTPGHPIPPTP
                     AQLQDCGWVANRWCELLPVPLELKHHLMTLDSPLLRLELVGDVLERLGIAP"
     gene            2456..4003
                     /gene="lysS"
                     /locus_tag="PK518_00370"
     CDS             2456..4003
                     /gene="lysS"
                     /locus_tag="PK518_00370"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRM47392.1"
                     /translation="MSEQNQAPIQPQDENQLIAERREKLKALRAGQAQGRGVAFPNDF
                     KPRDHAASLQQRYGSLAAEALETENISVSLGGRMMLKRVMGKASFATLQDGSLGDTGG
                     RIQLYVTRDAVGEEVYADFKRWDLGDIVGAEGQLMKTKTGELSVKVTTLRLLTKSLRP
                     MPDKFHGVADQEVKYRQRYVDLMTDDAARRRFVARSKAVGSIRNFMVEHGFLEVETPM
                     LHPIPGGANARPFVTHHNALEQEMFLRIAPELYLKRLVVGGFERVFEINRNFRNEGIS
                     VRHNPEFTMMEFYAAYWNYRDLMDFTEQLVRDAALKATGTLQLTYGGRAVDLSQPFQR
                     LTIREAIYQYTEAGAHVDDAAWLIGALKKLGMSEEKDRLSTRSLASLQVLYFEETVED
                     KLWQPTFIMEHPTEISPLARANDARPEVTERFELYITGREFGNGFSELNDAEDQAARF
                     NAQVAAKDSGDDEAMFFDHDFVRALEYGMPPTGGCGIGIDRLMMLLTDSPSIRDVILF
                     PALRREQ"
     gene            4014..4670
                     /locus_tag="PK518_00375"
     CDS             4014..4670
                     /locus_tag="PK518_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRM47393.1"
                     /translation="MLERLKTLLPPWMHDWLDIIVPGVQIALIVLAALLLQHLVRRII
                     RRASDHYQFPHELLTPINGVVRWLILGGAGLLALERLGVSAAVLWTAFTSFAAVGAVA
                     FFAAWSVLSNLFCAFLIFTVGPFRVGDHIELLDTAEKPGALGRVVDINLLYTTLEDAT
                     APEPGSLLQIPNALIFQRVLRRWPAGVPVPQGKLRPVPPSGGAATTESAAAPKGASFP
                     "
     gene            4867..5985
                     /locus_tag="PK518_00380"
     CDS             4867..5985
                     /locus_tag="PK518_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM47394.1"
                     /translation="MSIPFRMHSVVAALGLASAALLSTAHAQVKIAMVIPATGPVTQY
                     GDMIKEGVNTAVEQANAAGGINGKKIELVTVDDACEPKQGPVAANRVINDKIHYVVGP
                     VCSGAAIAAAPIYNNEGVVVVTPSATSPALTEGKNFHFIFRTIGRDDQQGPAAAKFIA
                     EKIKPKKVAILHDKQSYGQGIAASVRDDLKKAGVNVALFEGINAGDSDYSAVITKLKS
                     AGVDFVYYGGYHPEMGLLLRQAGEQGLKVKLMGPEGVGNPEVNAIAGPAVEGMLLTLP
                     ADFSTNPKNAAIVKAFKDKKRDAGGAFQMTAYAAAQVIIDSIKAVGDNPAKVADHMHK
                     ATFETPLGPISWNKSGDLKSFDFQIFEWHKDGSKSVAK"
     gene            6163..7089
                     /gene="livH"
                     /locus_tag="PK518_00385"
     CDS             6163..7089
                     /gene="livH"
                     /locus_tag="PK518_00385"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HRM47395.1"
                     /translation="MSEFLPQLIQQLFNGLSLGAIYALIAIGYTMVYGIIGMINFAHG
                     EIYMIGAYAGLVTLAAVGTQSGLPVAVLIALMLLVAVLVTGTYGFVVEQIAYKPLRNG
                     PRLVALISAIGMSIFLQNWVALGQGARDMAVPALLPGALRFGDEGGFEIFVPYARVLI
                     IAVTVVLMIALTLYIRHSRMGRASRACSQDMHMAGLLGIDVNRVISFTFILGAVLAAV
                     GGVLIALAVGKLNPFIGFIVGVKAFTAAVLGGIGSIPGAMLGGVLLGIAETFAAAYIS
                     SEYKDIVAFGLLVLILLFRPTGLLGKPEVEKV"
     gene            7089..8348
                     /locus_tag="PK518_00390"
     CDS             7089..8348
                     /locus_tag="PK518_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HRM47396.1"
                     /translation="MSAAITSSARPSWRQNLGHALTATVLTAVVVTPIFGLHLERAGV
                     RTIITQHWSNVAWACLLVFIVQMLRPMLAGSGVRLPWPKLPAIPARQRGVWIAAALLL
                     AVIWPFFGGRNAVDIATLVMIYVMLGLGLNVVVGFAGLLDLGFVGFYAVGAYTYALLF
                     HWAGWSFWEALPLAGAASALFGFVLGFPVLRLRGDYLAIVTLGFGEIIRLLLVNLTDF
                     TGGPDGISSIPKPTVFGLELARSPSVEGATTFHQFFGLEFNSMHMVIFLYLMALTLAV
                     VTLWISNRLIRMPIGRAWEALREDEIACRSLGLNPMKIKLSAFTLGAMFAGFGGAFFA
                     ARQGIVNPESFTFIESALILAIVVLGGMGSQLGVILAAILLTALPELAREFAEYRMLI
                     FGLVMILMMVWRPQGLLPMQRHHVEVK"
     gene            8348..9127
                     /gene="livG"
                     /locus_tag="PK518_00395"
     CDS             8348..9127
                     /gene="livG"
                     /locus_tag="PK518_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HRM47397.1"
                     /translation="MAGSDKNSFLQIEGLSMRFGGLLAVDGIAFDVKEREIFAIIGPN
                     GAGKTTVFNCISGFYRPTTGAIRLQGRDIAGQGSHSVALHGLVRTFQNVRLFKHMTAL
                     ENLLVAQHRHLSTNLLTGLFKTRGYRESEERALQNALFWLDYMGLREFANREAGNLAY
                     GHQRRLEIARCMVTQPRVLMLDEPAAGLNPQEKKDLQSLIEKLRQEYGVAVLLIEHDM
                     SLVMGVSERILVMEYGRPIALGTPEAIQKDERVIKAYLGEE"
     gene            9136..9837
                     /locus_tag="PK518_00400"
     CDS             9136..9837
                     /locus_tag="PK518_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47398.1"
                     /translation="MLELKGISTHYGAICAVNNVSLHVNQGEIVSLIGSNGAGKTSLL
                     MTLCGNPRASSGQVLYEGEDITNAPSHLIMRKGIAISPEGRRIFPALTVVENLQMGGF
                     FQSKDAIAEGMEYAFKLFPRLKDRANQRAGTMSGGEQQMLAIGRALMSKPRLLLLDEP
                     TLGLAPLIIAQIFEIIQAIRAQGVTVFLVEQNANRALSIADRGYVLETGRLVLEDTGA
                     NLLINPEVRKAYLGH"
     gene            complement(10065..10394)
                     /locus_tag="PK518_00405"
     CDS             complement(10065..10394)
                     /locus_tag="PK518_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HRM47399.1"
                     /translation="MSRLRLMEDLEADDPILSVVNLIDVFLVVIAALLLAVANSSVNP
                     FSTESVTIIKNPGKDNMEIIVKDGQKVDHYKASGEIGEGQGAKAGVAYRLKDGSMVYV
                     PEAGEAH"
     gene            complement(10391..10927)
                     /locus_tag="PK518_00410"
     CDS             complement(10391..10927)
                     /locus_tag="PK518_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRM47400.1"
                     /translation="MTANPIELAMYSASQLFLTPVLILIGLLFVHAFYALGAFIWQAW
                     QRHNGQAGGFELAAALARNPQMTLTELEALATKRMEFARIATRIAPMLGLVATMIPMG
                     PALMALSDGQMADVSRNLTVAFSAVILALLAASISYTVVNVRRRWYAVDLAAIEHDAP
                     QPQRDHDPVAVMALEGAR"
     assembly_gap    10963..11062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11229..15314)
                     /gene="cobN"
                     /locus_tag="PK518_00415"
     CDS             complement(11229..15314)
                     /gene="cobN"
                     /locus_tag="PK518_00415"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HRM47401.1"
                     /translation="MFQNQKLRWLLCTALLLLGGAARAASVLFVATGNVPQGKFHQLQ
                     EIARPHGIDVEVRYINSLPADVDAGLWAGRDAVFFDSYLQDEVRARLTRALPALKAPN
                     AWLYDQQPAWGGGLPEAVARRLIAYYGSGGRQNYEGFFATLAAQLKGGNALTAAPDPV
                     VFPKTGIYHPRLPGTVMADPVAFLRGQGVEMKAAQRKPVVAISLHQQYIGSMQTAYID
                     DMVARIEAGGAVALPFYTPMMGDGGFAKVLQPGGPGTPVLADVLINTQIMLNADERRE
                     EFERLGFPVIQAMTHRRGDEADWAASRQGVAAMDVPFYLAQAEYAGVTDIQVASAVRK
                     GDEQIMPIAAQAAAVAAKALNLVKLQRTPNADKNVAVMFWNYPAGEKNLSASFLNVPR
                     SLQNTLAAMVRAGYQTESPDEAMLITLLQRLLAPSYRPGLLEPLLRDGLAELLPVKDY
                     RAWLASLPQATQDELRRAWGDPEKSPWVLRQNGQAYFIIPRLQLGKVTLLPQPGRSGA
                     APGSTDARAKEKEIYHSTVDLPPHHYLATYLWTRQKNDALVHFGTHGTQEWLPGKERG
                     LSVHDAPLLALGDIPVAYPYIADNIGEATQAKRRGRATIISHQTPPFAPGGLHDALTQ
                     MHDLLHQWQAQDEGAVRDRMAADLLAAARKERVIADMGWTEARVKTDFAGFVHELHNH
                     LHELAQTAQPQGLHRLGQAPEELHRLGTVLLTLGKNFWEAAARRAGVPLADLDEALVG
                     PWDKLAQTVPYQLLQKHVVAGEPMTGLPADLQQALAQAQTAYDNLGAQGELKGLLDVL
                     AGRYLPTSYGGDPIKNPDAYPTGRNLYGFDPSRVPTKQAWEAGKLAADNLLAEHRKLH
                     GKQPSKLTFTLWSVETMRHFGMLEAQALWLLGVEPVWDKGGRVTGVKLVPREQLGRPR
                     VDVVLSATGLYRDHFPNVMKQLAQAVLLASRATEIDNPVAQNAQRIAQKLIAQGADAK
                     AAEMAGATRIFASESGRYGTGLDEATQATDTWKGKKEGDRKMAELYLSKMQFAYGPDE
                     KEWGQAGVAGVGDGKGGKGGKGGKGVNLYAEHLSGTEGAVLSRSSNLYGMLTTDDPFQ
                     YLGGIAMAVRHLDGKAPELYISNLRGSGSGKVEGAAQFLAKELATRQFHPGYIQGLMK
                     EGYAGTLQVLDATNNFWGWTATAREIVRDDQWQEMVDVYVRDKHKLGLKKWFESKNPH
                     ALAQTMERMLEAARQGYWKADAATVKELKERWRDLSERYDVQSDNARFSEFVAEGAAQ
                     AGFGLQATAAAAAPARAAAQPQEQAEAQPEAAPPEPPPPPMVTGMRMQKVQPKELPPF
                     NPATLLFAVALAGVSCAGAWQQRRRSGPFPLWGKVGMGAAG"
     gene            complement(15374..17326)
                     /locus_tag="PK518_00420"
     CDS             complement(15374..17326)
                     /locus_tag="PK518_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRM47402.1"
                     /translation="MRIPFAVPCRRHPLALAALLLATGAAQAQTETLALAPAQGLPAV
                     TVTATMTEQDARTAPASVTVISAEELAEKNAGDLLDAVRGAPGITLQGRQVGGRKTLA
                     LRGLEGKHTLTLIDGRRISASDDVIGHSDYQYGWLPISAIERIEIIRGPMSALYGSEA
                     LGGVINVISKKPKDHWSGSIGVSGGTGLKSDAASQFGSSVYAAGPLGQRARLSVNAEY
                     ARSNPTPDIDDLRYSEVEGRRPRNLGVNAEFDLTASQRLQAGISDGQERRLYDDVNTA
                     GRAYVNRYDLDRRQTHVGWTGDFGDLKAQLRAYRSEMSVINSRSNGVAPTRPQDMQDD
                     VVDGHVTLRRGSHNIALGGEWRSEELVNAGLKGGRDDVTHKALFVQDEFSLGANLMAT
                     LGLRADHHGLFGSELSPRAYLVWEASPQLVVKGGWGHAFKAPTLKQISPNYVGAEGPH
                     SFLGNANVKPETSNSLEIGADWQASSALSLRATAFHTEVKDLITYKLLQQIGIRRVYQ
                     YDNIDAARIQGLEAGFTLQLSRQLSWGSDATLLHTRDKATGKRLNDRPTTSVASHLTW
                     KRGGWDARMGLQYTGSQENYGNKLPAYTLFNVSAGYAMKLNATQNLHLRAGLENLGNV
                     RLAEKSPNFGYAELGRRVFVSARLDF"
     gene            17664..18101
                     /locus_tag="PK518_00425"
     CDS             17664..18101
                     /locus_tag="PK518_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47403.1"
                     /translation="MPLLRNAPRLARLLLACFALSLAVAMAAPLVQARPMERVCTASG
                     DERWVAAGGNQVGTLPYEGHQFSCALCLPPMLPGAQAATLPQPQGQPFMAQALRVRIG
                     HVPALSRAAFPPRAPPAPAPSPCNLSECGGAICAPPRCAHGPG"
     gene            18145..18630
                     /locus_tag="PK518_00430"
     CDS             18145..18630
                     /locus_tag="PK518_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRM47404.1"
                     /translation="MNFQPIATRITLAAALLLTGFAHAQVSVQDAWVRATVPQQKATG
                     AFMRLTAAQDMRLVAASSPVAGITEVHEMKMVDNVMKMRAVPALDLPAGQAVELKPGG
                     YHIMLLDLKQPVAQGASVPLTLVFEGKDGQRQTQELQAPVRALGATAAPAMGHGKHSG
                     H"
     gene            18794..20026
                     /locus_tag="PK518_00435"
     CDS             18794..20026
                     /locus_tag="PK518_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRM47405.1"
                     /translation="MHPDAQHLWRGPRWALAILLALLGMVGPFSIDTYLPAFPAISQA
                     LGATPVQMQQTLSAYLFAFAFMALFHGALSDSLGRRPVVLWGLALFTLASAGCALAQT
                     TGQLVAFRALQGLSAGAGIVVSRAVIRDLFPPAQAQQVMSQVTIFFGVAPAIAPIVGG
                     WLSVHLGWHSVFWFLTGVGLFLWLANWRLLPESLPPEGRQSIHLGHLMRGYWELISSP
                     RFMLLALASGVPFNGMFLYVLSAPAFLGDLLGLAPQQFFWFFLCTISGIMGGAWMSGR
                     LAGRIPPKHQIRHGFVIMLAMGLLNLGANLLFPAHVGWALWPIGIFSFGWALMVPVVT
                     LLVLDLFPARRGMASSLQAFVGSTANGLVAGVVAPLVMHSTVLLATASLLMMCVGLVS
                     WIYLHHRWPEIGRTATHS"
     gene            complement(20034..21491)
                     /locus_tag="PK518_00440"
     CDS             complement(20034..21491)
                     /locus_tag="PK518_00440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM47406.1"
                     /translation="MLPVPSTDFEHMFELAPVSLWLEDYSAVRRLFEQWRAAGVVDVV
                     PHLRRHPECVRAYGASIRLLRVNRRTLELFAAPDQATLEASLDQVFRGDMFESAIAEI
                     AQLWDGVPEYANQTVNYALDGRRLDVRIRGRILPGYEGCWSRVLVSLEDVTERVQARL
                     RLERSEKYARGLFEHSPVSLWVEDFSAVKPLLDEVRAQGISDLKTFLKVHPEFVSRCM
                     REIRVLDVNQLTLSMFGAPDKQTLLAGLDKVFRGEMHDSFSEQLLDLWEGKLFQQREA
                     VNYSLIGEPVHLHMQFAVLQEHRHDWGMVLLSMVDISARKKAEAYLEYLGKHDVLTGL
                     RNRAYYVEELNRLTRKGPWPVAVIAIDLNGLKAINDEHGHAAGDAMLRRAGEVLAKAV
                     DASACTARIGGDEFCVLLPMTDERGAQALMERIISLQELNNQFYPGQALSLSMGVAVA
                     MSGDQIEAAAHQADQAMYAEKARYYAAMGLDRRHA"
     gene            complement(21612..23495)
                     /locus_tag="PK518_00445"
     CDS             complement(21612..23495)
                     /locus_tag="PK518_00445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM47407.1"
                     /translation="MTEQVLLQGECAPADSVISAINAVPEAAVPNGFVELGLAPELVQ
                     AVADLGYTQPTAVQDKAIPLAMGAGAEDGRFIDLMVSSQTGSGKTAAFLLPVLHTLLR
                     QQAEAEAAQRAEYERAALEAAARGEAAPKRPKRKNPMQARNFKAAVPGALVLCPTREL
                     AQQVAHDAIDLVKHCKGLRIANVVGGMPYQMQIAKLQNANLVVATPGRLLDLQRSQQI
                     KLDQVQFLVVDEADRMLDLGFSDDLAEINELTAQRKQTMMFSATFAPRIQQLAMRVMH
                     DGGASVKKIQIDSPQEKHSNIQQKLFWADNAQHKRKLLDHWLRDASIDQAIVFASTQV
                     ECDGLANDLQQDGFSAVALHGALSQGLRNRRLMALRNGQVQILVATDVAARGIDVPTI
                     THVFNFGLPMKAEDYTHRIGRTGRAGRDGLAVTFAEFRDRRKIFDIEGYSRQQFKAEV
                     VEGLEPTQRFPQGGRPDFAGRGGREGGGRGRDQRFGGAGRGHGDRGGFGDRGGFGERG
                     PRGGFGGDRGGFGARRDGDGYGRKGGFGETSRFGETSRFGETSRFGDAPRFNDGPRHA
                     HAPHGHHHGRGDGGAPRGDFGRKSDFAKPGFGKGGFAKPHGGTGKSFTPHEARKRPAR
                     NDR"
     gene            23786..25324
                     /locus_tag="PK518_00450"
     CDS             23786..25324
                     /locus_tag="PK518_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM47408.1"
                     /translation="MHLCTLQEDAMQLPLNIVAHGSESLQQQITQQIRCLIHDGRLAP
                     GTRMPATRELASDLGVSRNTVIGAFQRLIAEGCLESREPVGTFVAQRIIVDRVAPPVV
                     TGQATPPFTSAARQDTGMHRPQPRLRPPQHWQPHHVARPHAGQVGLDFWVGRPDARLF
                     PLRSWGMLLARALHSQPHQLCEYGDPQGMLALREAIASHIGATRGIATDAGRVLITNG
                     IQEGLSLLAQLLIAPGVEVAVENPCYMGASRVFAAHGGVLAGVPVDDQGMRTDALPRK
                     AALAYVTPSHQYPLGGTLPLERREALLAWARNCGAYVLEDDYDSDFFYDRAPLLALKS
                     LDHHDQVVYLGTFSKTLGAGLRIGYMVLPHSLCQAAVNAKAMLNNSQSWLEQAALAAF
                     MAEGGYEHHVRRLRRLYADRRDHLRTALGQWLPDWQVQGAGSGMHLVARVPPNAAPIH
                     ELERAAHAQGVGIYGVERGNGQLLNVAADHPWRRIVLLGYASLDEPQISDALLRLRQV
                     FPHS"
     gene            complement(25278..26579)
                     /gene="ccmI"
                     /locus_tag="PK518_00455"
     CDS             complement(25278..26579)
                     /gene="ccmI"
                     /locus_tag="PK518_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRM47409.1"
                     /translation="MTGFVLLAAAMLALALAFVVLPLLRTRAAGTGAGANLTVLADAM
                     RELDEDLAAGRIDALEHGRSGDALRRQALAADAMRQQQQERSRGANRGAALGLGIALP
                     LMAVALYLVVGQPGALMLAGQSPPVHERQMDAAVQALAQRLESTGGDADGWMLLARSQ
                     LSAGRAAEAVRAYAKASELRPDDANLLVEYANAVAMTQQRDLSGQPQDLLARALRIEA
                     DNLNALALAGAAALQAGDRDGALQHWSRLEQLVAADPQDLERVRVLLARARGEAPPPP
                     AALPAGHPDTAPVMARVADQAPASKAGISGTVTVASELAARVAPTDTLFIFARAPGGP
                     PMPVAAMRTRAGGWPVAFQLDDSSAMGQGRALSSFSQVDIVARISRLGSPVAQAGDIE
                     GRLERVALGSSNLRLVLDKVVGRSAELKNGEKPASASTAHR"
     gene            complement(26596..28623)
                     /locus_tag="PK518_00460"
     CDS             complement(26596..28623)
                     /locus_tag="PK518_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRM47410.1"
                     /translation="MSAELGQFALVLALVLALAQGVLPLVGAARGNGDWMAFARPAAR
                     GQCLFVAMAFACLTHAFVSNDFSVLYVVQHSNSALPLPYRIAGVWGGHEGSILLWALI
                     LGGWSVAVSFFSRHLDEPARARILGVMGLISVGFLAFILFTSNPFSRLLPAAPDGQDL
                     NPQLQDPGLIFHPPLLYMGYVGTVVAFAFAISALLAGRLDAAWARWSRPWATVSWCFL
                     TLGIALGSAWAYYELGWGGWWFWDPVENASLMPWLVSTALLHSLAVTEKRGGFRMWTA
                     LLAIIAFALSLLGTFLVRSGVLTSVHAFATDPSRGIYILGFLIIVIGGSLILFAWRAP
                     LVGGGSRFDLLSREAMLLANNLLLLVACAAVLLGTLYPLLLDALGLGKLSVGPPYFDS
                     VLVPLMAPALFLMGVGPLARWGGDQVPDLARLLRWALAVSLASALLAPWLMGGWSALV
                     GLGLFFAFWIFATTVLSLIRQWRPMGRDGLWLRVRKQPRSYYGMLLAHCGLAVFIVGV
                     TLVKGYELERDVTMEPGLSTTLAGYEFRFDGVRRQQGPNYLADVATLQVLRGDRVVAT
                     LQPERRLYTAGRNPSPMTEAAIHSGLARDLYAALGESLGGNRWVVRVYYKPFIAWIWW
                     GCGFMALGGFWAVLDRRYRPRALAAAQPAPPGAVVRAERGRGTVALKEARR"
     gene            complement(28620..29663)
                     /gene="haoB"
                     /locus_tag="PK518_00465"
     CDS             complement(28620..29663)
                     /gene="haoB"
                     /locus_tag="PK518_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine oxidation protein HaoB"
                     /protein_id="HRM47411.1"
                     /translation="MLVAHAHDQATFPAHHRQADWKRRFALFSIAVGALLISVAGLFA
                     WKHVSSPASYHYELGAVVPAGELDAWASLAERQVVVRRASIIAADQQPLAELELAEVS
                     SGPVLLKWKSRVDDPFLTVGTSAQDVSALAQVLQRHMPKDGLVLGWWDTSRQLQLLGG
                     TPVRFGKHLGLPLFVPQRWRSQSADIESIERAFWKAGNAGEERVQFQRFANALLSDEA
                     SGMAELQAIAGGKPAVLVLHVRDAILLGQMDSRRLGVAFTDLGALSDVHGMVRRVHDW
                     LDHNRYPAYAMLQDKDSPVRAVALTDAASGKTLAARLLPFMGNDQQDVAGATLVYRVG
                     GFVVYELAPQARP"
     gene            complement(29663..31456)
                     /locus_tag="PK518_00470"
     CDS             complement(29663..31456)
                     /locus_tag="PK518_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRM47412.1"
                     /translation="MLTRWLSLLIAAVALLASHMPAQARIWDSVPDEQLQALQLSREA
                     SPKELFEALSKRYKAELNKGKLARYWEPIPMDMYLAPSLFYKPPDVNIDVTREQCVSC
                     HTSVTHGWVKSWEKSVHANLDAIRQLPASDVRAYKKDIIAEVEDNLRGQGLLAKTEPL
                     KEVQCTDCHMGIGAKGGNHVKDLRMPDRAVCGSCHVRQFAEAESERDTLVWPQKQWDQ
                     GHPSHTVDYRANVETATWAVLMNEGKGPVAASCTMCHTNQTKCDNCHTRHEFSTVESR
                     KPEACATCHNGVDHNEFEQYMYSKHGTAYQTLGNQWDWNIRLADQLGHKNGATAPTCQ
                     TCHFEYKGQYSHNVVRKVRWGFLPFKSIADNLEHPWFKERKDAWAATCSQCHAPRFAQ
                     TYLTMMDDGIKLGTGLVEQSRKVMQKLYDDKLLVGQKTNRTPLPAPEKDEPGGFSSLF
                     FATNNQTTVADRTFAEMWEQHVARYMKGLEHVNPGGWTYSHGWSDLIKDQTIINEQDT
                     VLRENARMEQRLRKLESGRAKTSFLNRSPDDGPQSVLAAVTDDPLTSSLALSLLGGTL
                     VVGGASTVRRRRRLPGRQASDATTLPEQETR"
     gene            complement(31815..32933)
                     /locus_tag="PK518_00475"
     CDS             complement(31815..32933)
                     /locus_tag="PK518_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM47413.1"
                     /translation="MKSTLVAAAALVCASGALAQEQVVKIGHSGPLSGPNTFAGRDND
                     NGVRLAIEDLNARKINVGGKTLKFELVSEDDQCDAKAGVAVAQKFVDSGVRYVMGPYC
                     SGVTIPASRVYDNGGTMVSTVGTNPKITQGGYKNLFRIVASDEKAGSNMAGYAANVLK
                     VKKVGVIDDRTAFGQGLADEFAKEAQKLGLTVVGREFTTDKATDFMAILTNMKAKQPE
                     VIFYGGYAPQAAPMARQMKQLGINAKLLGGDTLCSPEMPKLGADAVNNVVYCAYAGML
                     TDSDAGAKAFQEKFKKRFGQNPDVYGPFYYDQVMNIGEAMQKSGSIDPAKVGAYMHQQ
                     TYKGVMGEYAYDDKGNRIKAPIVVMTFEGGKAKPLANY"
     gene            complement(33072..33650)
                     /gene="orn"
                     /locus_tag="PK518_00480"
     CDS             complement(33072..33650)
                     /gene="orn"
                     /locus_tag="PK518_00480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRM47414.1"
                     /translation="MSETVNPTAPTLAKSDQNLVWLDCEMSGLDPEKEYLLEIAVVVT
                     GPSLEPRVEGPVFAIHQSDAVLDAMDAWNKGTHGKSGLIDKVRASTVSEADAEQQLID
                     FLSLYVPKGCVPMCGNSIGQDRRFLVKYMPRLEAFFHYRNVDVSTLKELAKRWKPEAY
                     ASFKKAQRHTALADVHESIDELQHYRQQLLAL"
     gene            33683..34999
                     /locus_tag="PK518_00485"
     CDS             33683..34999
                     /locus_tag="PK518_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRM47415.1"
                     /translation="MPPSSTAPFLSPSTLTTLVFAALLLAGLALRLWLATRQIRHVAQ
                     HRGAVPAAFAERITLAAHQKAADYTIAKARFGLLSMALSTAVVLGWTLLGGLDALNQQ
                     LLAWLGGGMPQQLALLAGFALIGGALDLPASLYQTFRIEQRFGFNQMTPRLWLTDLVK
                     STLIGAVIGLPIAALILWLMGAAGPLWWLWAWGVWMGFNLLLMVVFPVFIAPLFNKFQ
                     PLADEALKARVTALMQRCGFAAKGLFVMDGSRRSAHANAYFTGFGAAKRVVFFDTLLR
                     QLSPAEVEAVLAHELGHFKHKHVTRRMLGMFAVSLAGFALLGWLSTRGWFYTGLGVQP
                     NLMIEGLPGSAPNDALALLLFLLAVPVLTLFAQPLMAQISRHQEFQADAYAVSQASGT
                     ELASALLKLYQDNASTLTPDPVYVKFYYSHPPATERLARLPVAVQS"
     gene            34996..35337
                     /locus_tag="PK518_00490"
     CDS             34996..35337
                     /locus_tag="PK518_00490"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRM47416.1"
                     /translation="MTSMFPKKDWSTQTRRALSATEIVVKLTELPGWQLSGDGADVAI
                     EKTYRFANYHETMAFVNAVAFIAHAQDHHPDLSVHYARCVVRLNTHDVGGISATDIDC
                     ATRIDALLAAP"
     gene            35334..36290
                     /gene="rsgA"
                     /locus_tag="PK518_00495"
     CDS             35334..36290
                     /gene="rsgA"
                     /locus_tag="PK518_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRM47417.1"
                     /translation="MTRSEGLVVASHGRHCVVESADGVRRICHPRGKKSQAVVGDRVL
                     WQAAAPGQAQGHGDEGTIEKVLERRNLFYRQDEVRTKSFAANLDQVLILIAAEPVFSE
                     SQLVRALIAAEAAGITPLIALNKSDLVEPFAQAWQRLWPYRHMGGEQDTRHHYRVLPL
                     SLTQSGAVDRDALLPLLAGKTTLILGPSGSGKSTLVNLLVPGAAAQTGEISQALNSGR
                     HTTTTTSLYWIDGARTTALIDSPGFQEFGLRHLAPTDLARCMPDIAAHAGGCRFYNCS
                     HLHEPGCAVMAQVSESQDAQAISLQRYRIYSELFAELSQSAY"
     gene            complement(36358..37347)
                     /locus_tag="PK518_00500"
     CDS             complement(36358..37347)
                     /locus_tag="PK518_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HRM47418.1"
                     /translation="MSFFAILFALLIEQARPLARSNPIHAGLRAWALSVCRSFDAGQS
                     RHGWVAWSLAVLVPPAFVLAVHWALLHWLGWPLALLWSVAVLYLTLGFRQFSHHFTAI
                     RDALEDGDAAEARALLARWKQVDASELPRSEIVRHVIEYSVIAAHRHVFGVLAWYSVF
                     AALGLGPTGAVLYRLAEFVARYWGRGSRVVTAHPPSESLQRAAEQAWMVIDWLPARLT
                     AMSFAVVGSFEEAIDGWRFHAQRFPNDNDGVVLAATAGAINVRLGGEALRSRAGAELA
                     ADFEAGADMADSSSTPGREPEVGHLRSVVGLVWRSVVVWMLLLALLTLARLLG"
     gene            complement(37394..38077)
                     /locus_tag="PK518_00505"
     CDS             complement(37394..38077)
                     /locus_tag="PK518_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRM47419.1"
                     /translation="MSSLSSLPDFDPRTVPLVGVDGHLPAVPAAAQTPDALRRRFATP
                     PLWTPEIVREKKFVQRPPAEAAVLVPIVQRERPTVLLTERTAHLSTHKGQVAFPGGRS
                     DPEDLDAAATALRETHEEVGLEAGSVEVLGQLPIYVTGSSFIVTPVVALVRPDFVLAP
                     NPDEVADVFEVPLDFLLNPAHHERHALEWQGLRREWFAMPYQDGARQRYIWGATAAML
                     RNFYRFMLA"
     gene            complement(38201..38584)
                     /gene="rplS"
                     /locus_tag="PK518_00510"
     CDS             complement(38201..38584)
                     /gene="rplS"
                     /locus_tag="PK518_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRM47420.1"
                     /translation="MNLIQILEQEEIARLNKTIPAFAPGDTVIVSVNVVEGSRKRVQA
                     YEGVVIAKRNRGLNSGFTVRKISSGEGVERTFQTYSPLIAGIEVKRRGDVRRAKLYYL
                     RQRSGKSARIKEKLPSRVKVAATAA"
     gene            complement(38715..39470)
                     /gene="trmD"
                     /locus_tag="PK518_00515"
     CDS             complement(38715..39470)
                     /gene="trmD"
                     /locus_tag="PK518_00515"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRM47421.1"
                     /translation="MRFDVITLFPELFEPFLASGVTRRAYATGQVQVRLWSLRDHAEG
                     NYRRVDDRPFGGGPGMVMMAEPLARCLAAIRADRAEAQAAPLVLFSPIGRRLDHAAVQ
                     DWSAGAGAVLLCGRYEGVDQRFIDAHVDMQISLGDFVLSGGEIAAMALLDAVARLQPG
                     VLHDEGSHQLDSFNPALDGLLDCPHYTRPEQWQGRGVPAPLLSGHHAQIERWRRDQRL
                     VTTARHRPDLLQAARERGLLTRADEAVLAQNPL"
     gene            complement(39478..40050)
                     /gene="rimM"
                     /locus_tag="PK518_00520"
     CDS             complement(39478..40050)
                     /gene="rimM"
                     /locus_tag="PK518_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518084.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRM47422.1"
                     /translation="MPQPPILEASELPPDAVEVGRITDAWGVKGWFKVLPYSSDPEAL
                     LASRAWFLQPAERGAKSFFAGTVLLPIRQARTHSDTIVAWAQGVDDRDGAEALRGARI
                     FVPRASFPAVGDDEYYWVDLIGLAVVNREGVPLGTVRDLLPTGPQTTLVLGYEHEGKA
                     QERMIPFVSAFVDKVDLAGRCIRVDWQPDY"
     gene            complement(40144..40395)
                     /gene="rpsP"
                     /locus_tag="PK518_00525"
     CDS             complement(40144..40395)
                     /gene="rpsP"
                     /locus_tag="PK518_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRM47423.1"
                     /translation="MVVIRLSRGGSKGRPFFNIVVADKRVRRDGRFIERLGFYNPSAK
                     GGEETLRIAQDRLTYWKGVGAQASPTVDRLIKQAAKQVA"
     gene            40519..41040
                     /locus_tag="PK518_00530"
     CDS             40519..41040
                     /locus_tag="PK518_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRM47424.1"
                     /translation="MPTIRASIDGDIAAITAIYAHHVLHGTGTFEVDPPSSQDMAARR
                     AEVLARGLPHLVAEEGGQVLGFAYCNWFKPRPAYRYSAEDSVYVADAARGLGVGRLLL
                     DALAEQAQAAGMRKLLAVIGDSANAGSIALHRAAGFTEVGVMRSVGWKFGAWRDIVLM
                     EKPLGAGDSSAPE"
     gene            41063..41509
                     /locus_tag="PK518_00535"
     CDS             41063..41509
                     /locus_tag="PK518_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47425.1"
                     /translation="MKNKTLATWLAFLGGPLGLHRFYLFGFGDMLGWLLPVPTALGVY
                     GLQRVQQWGQDDQLSWLLIPLLGFTIAGCALCAIVYGLMAPEKWNARFNQTAAADAAP
                     GRTNWFTVFAVALSLLVGTAVLMASIAFSLQRYYEYQIEEARKISQ"
     gene            complement(41565..42575)
                     /locus_tag="PK518_00540"
     CDS             complement(41565..42575)
                     /locus_tag="PK518_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRM47426.1"
                     /translation="MDTVQTALWVVVVLVALAILFDFMNGFHDAANSIATVVSTGVLK
                     PTQAVVFAAFFNFVAIFVFHLSVAATIGKGIADPGVVDTHVIFGALVGAITWNVITWY
                     YGIPSSSSHALIGGIVGAVVAKSGSGALMASGIIKTVAFIFISPLLGMVLGSAMMVLV
                     AWVFRRTRPIKVDKWFRRLQLVSAGAYSLGHGGNDAQKTIGIIWLLLIATGYASAADS
                     APPTWVIVSCYAAIGLGTMFGGWRIVKTMGQKITKLKPVGGFCAESGGALTLFFATAL
                     GVPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWILTIPASAFVAAIAYWISLQ
                     LY"
     gene            complement(42601..43248)
                     /locus_tag="PK518_00545"
     CDS             complement(42601..43248)
                     /locus_tag="PK518_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRM47427.1"
                     /translation="MLFGKLLPREGNFFEMFNQHADRIVEAARAFSQLVANYSDTHLR
                     DQYNQDVDNAERAADRVTHEVNRTLHQTFITPIDREQIHSLINTMDDVADLIQDSAET
                     MALYDIRHMTEEISRLTDLSVKCCERVRDAVKMLSRIADPAVAEAALKTCEEIDRLEG
                     DADRVLRSAMSKLFREETDVRELIKLKAIYELLETITDKCEDVANLIEGVILENS"
     gene            43481..44062
                     /locus_tag="PK518_00550"
     CDS             43481..44062
                     /locus_tag="PK518_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRM47428.1"
                     /translation="MPSTLRQLLCATLLTGLAAAGLAQAPMPRAAGMYGGYVGLGAGA
                     SRYDLRSGSGGFDFDDGGSAIKLYAGAFFHPNLGLELGYLDLGKARRIGGNSSARGLN
                     FSLVGRLPLGTQLDLQGKLGTFYGRTDTRGVGGYGVQLGKGSGFGLSYGAGLRWAFTP
                     QWSAVLQWERQRLHFADRLSDVDAATLGLQYNY"
     gene            complement(44085..44447)
                     /locus_tag="PK518_00555"
     CDS             complement(44085..44447)
                     /locus_tag="PK518_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRM47429.1"
                     /translation="MHVLQSLPIPLQTILLLVASNVFMTLAWYGHLKNLATAPWYVAA
                     LVSWGIALFEYLLQVPANRIGHAQFSVGQLKIMQEVITLTVFVPFAVFYLNEPLKWDY
                     LWAGLCLVGAVYFIFRSA"
     gene            complement(44562..45293)
                     /locus_tag="PK518_00560"
     CDS             complement(44562..45293)
                     /locus_tag="PK518_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47430.1"
                     /translation="MADTNNKQVLLKVTGLKVAYGGIQAVKGVDMEVREGELVTLIGS
                     NGAGKTTSMKAITGGLGMNDGDIEYLGRSIKGRGAWDLVRDGLVMVPEGRGVFARMTI
                     TENLLMGAYTRNDKAGIEADVEKMFNIFPRLRERKDQLAGTMSGGEQQMLAMGRALMS
                     QPKVLLLDEPSMGLSPIMVDKIFEVVRDVYALGVTIVLVEQNASRALAIADRGYVMES
                     GLITMTGAGQDLLSDPRVRAAYLGE"
     gene            complement(45295..46077)
                     /locus_tag="PK518_00565"
     CDS             complement(45295..46077)
                     /locus_tag="PK518_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47431.1"
                     /translation="MTDTSKDVVLKVAGISKRFGGLQALSGVGITIQRGQVYGLIGPN
                     GAGKTTFFNVITGLYTPDAGTFELAGRPYEPTAVHKVAQAGIARTFQNIRLFAEMTAL
                     ENVMVGRHIRTHSGLFGAVFRTQGFKQEEAAIAKRAQELLDYVGVGKYADFKARTLSY
                     GDQRRLEIARALATDPQLIALDEPAAGMNATEKVQLRELIERIRQDNRTILIIEHDVK
                     LIMGLCDRVTVLDYGKQIAEGTPAEVQKNEKVIEAYLGTGGH"
     gene            complement(46099..47175)
                     /locus_tag="PK518_00570"
     CDS             complement(46099..47175)
                     /locus_tag="PK518_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47432.1"
                     /translation="MKNNKLQWILGGIALLVLPLVLQYFGNAWVRIADLALLYVMLAL
                     GLNIVVGYAGLLDLGYVAFYAVGAYMFGLLASPHLTENFAWFAAHFPDGMHLSLWVVI
                     PLALVLAACTGVLLGIPVLKLRGDYLAIVTLGFGEIIRIFLNNLDHPVNLTNGPKGLG
                     QIDSVKIFGLDLAKRQELFGFDINSVTMYYYLFLALVLITIVICYRLQDSRIGRAWMA
                     IREDEIAAKAMGINTRNMKLLAFGMGASFGGVSGAMFAAFQGFVSPESFSLMESVMIV
                     AMVVLGGLGHIPGVILGAVLLSALPEVLRYVAGPLQAMTDGRLDASILRQLLIALAMI
                     IIMLLRPRGLWPSPEHGKSLASKS"
     gene            complement(47190..48119)
                     /locus_tag="PK518_00575"
     CDS             complement(47190..48119)
                     /locus_tag="PK518_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM47433.1"
                     /translation="MDILLQQIINGLVLGSMYALIALGYTMVYGIIQLINFAHGEVLM
                     IGALTSWSCIGLMQDAMPGAPGWLVLLLATVIACVVAAALNYTIEKVAYKRLRNSPRL
                     APLITAIGMSILLQTLAMIIWKPNYKPYPTLLPAAPFEVGGAVITTTQVLILAVTVIA
                     LAAMVYLVSYTRLGRAMRATAENPRVAALMGVKPDMVISATFIIGAVLAAIAGIMWAA
                     NYGTAQHTMGFLPGLKAFTAAVFGGIGNLTGAVVGGVLLGLIEAIGSGYIGTLTGGVL
                     GSHYTDIFAFIVLIIILTLRPSGLLGERVADRA"
     gene            complement(48316..49623)
                     /locus_tag="PK518_00580"
     CDS             complement(48316..49623)
                     /locus_tag="PK518_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRM47434.1"
                     /translation="MNAAAAHQPLAERLRPRTLGEVVGQQHVLGPGMPLRLAFESGRP
                     HSCILWGPPGVGKTTIARLMAQAFDAQFISISAVLGGVKDIRDAVQLAESARDGLMGQ
                     RTIVFVDEVHRFNKSQQDAFLPHVESGLFTFVGATTENPSFEVNSALLSRAVVYVLQP
                     LGSEDLKQIIAKAQNLQALPAIENEATERLIAYADGDARRLLNTLETLAITASQAGLA
                     LIEDDWVVRVLGERMRRYDKGGEQFYDTISALHKSVRGSDPDAALYWLVRMLDGGADP
                     RYMARRIVRMAWEDVGLADPRAMQIANEAAQTYERLGSPEGELALAQAVLYLAVAPKS
                     NAGYMAYNKARAFVKQDGTRPVPMHLRNAPTQLMKQLDYGKGYRYAHDEEGGFAAGES
                     YLPDGMEPTGFYAPVPRGLEIKIGQKLDELRARNSAAKLPAQK"
     gene            49704..50318
                     /locus_tag="PK518_00585"
     CDS             49704..50318
                     /locus_tag="PK518_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRM47435.1"
                     /translation="MPRHESLHALLHRVRACTICADYLPLGPRPVLQAGSGARILIAG
                     QAPGRKVHASGIPFDDASGQRLRDWLGLTPEQFYDPTQVAILPMGFCYPGKGISGDAP
                     PRPECAPAWRAQLLAHLPRLAFTVVLGQYALAWHMPEERSGVTQAVQNWQRHWPAAVV
                     LPHPSPRNNGWLKRNPWFETQLLPEVRARVIAVLATKGRHDRTT"
     gene            50563..50718
                     /locus_tag="PK518_00590"
     CDS             50563..50718
                     /locus_tag="PK518_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47436.1"
                     /translation="MSHHDDENESVPLIQQLLDSPFLLLFLGVMIPMVVYNLWGVLDI
                     LTIPLAK"
     gene            50733..51272
                     /locus_tag="PK518_00595"
     CDS             50733..51272
                     /locus_tag="PK518_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HRM47437.1"
                     /translation="MSAILPPSERLWWKHPLDRVEGLWILISFVWCMVMFFMMVGWHI
                     YGKQNLSTETYKTTPEKFAAQAQAAVDKWTVRTELEQKIPVVAPPEGADVYMIARLWN
                     FWPILELEKGKTYKLHLSSMDYNHGFSLQPTNINIQVVPGYEHVIKVTPNESGTFSVV
                     CNEFCGIGHHEMVGRIYVK"
     gene            51286..52980
                     /locus_tag="PK518_00600"
     CDS             51286..52980
                     /locus_tag="PK518_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRM47438.1"
                     /translation="MTTTYRTCPRTGLQFEAQAEKLMIANAFMAVVALLVGGLLAIGV
                     VLTRWPAVHWLPADTFYMVLTAHGIDMLIFWIIFFEVAVLYFASSTLLRCRIATPKIA
                     WLGFALMLIGAVWNNIVVFQGESSVMMTSYVPMMAHWSFYLSLILFAVGALVACFVFF
                     GTLVVAKRDKTYEGSVPLVTFGATTAAIIAVFTILSGAGVLVPTFFVSIGLLESVDPL
                     MYRVVWWAFGHSSQQINVAAHISIWYAVAAIAFGAKPMSERVSRGAFLLYILFLQLAS
                     AHHILADPGVSTEWKVVNTSYFMYFAVLGSMIHALSIPGAMEVAQRARGYNKGLFEWL
                     RKAPWSNPTFSGVFIALVGFGFLGGTTGVMMGTEQLNLLIHNTIYVPGHFHATVVVGT
                     TLTFMALTYFLIPSLFRREMIAPGLARLQPYLFGFSMYFFCLVMMGAGTLGVSRRHWD
                     MAFSGNALAYEWPGAAYLMMGLVGISGVAAIAGGVIYVYVTVGSLLWGKKLDAGVTSP
                     KFTPVARAAPTAAAQTYGSAGFVAPGTFVLAMFFLVTFVLYYFVNWKYLGQLWGLS"
     gene            52997..53896
                     /gene="cyoE"
                     /locus_tag="PK518_00605"
     CDS             52997..53896
                     /gene="cyoE"
                     /locus_tag="PK518_00605"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRM47439.1"
                     /translation="MHTTTLKPAGRSTAQMARDVISLFKLRIGVLIMITALVGMAVTP
                     GPAPSFVQALVLAISVLMASASAGAFNQYVEFESDRLMRRTSRRAFATGALPHHPAWL
                     VLMAVLLAVAVASAWLVLNAVAALHVFLGAFFYGVVYTLWLKRRTAMNIVIGGLAGSF
                     AVLAGAAAVDPGLGPLPWLLALVLFLWTPPHFWSLAIANSADYAQAGVPMLPVVVGPE
                     RAARTVLLSTIALFLASLAPLAFGAGWVYGAGALVGGLHFVRKSWQLARKPERATAMG
                     SFFASLVQLSLLLVAASIDALMR"
     gene            53991..54770
                     /locus_tag="PK518_00610"
     CDS             53991..54770
                     /locus_tag="PK518_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRM47440.1"
                     /translation="MASSSNTTDLGLDREAALRTSQAAIGRKVGDHILLNREGVPTSL
                     ASYRGKPLLVSFIYTGCFQVCPTTTRSLQEAVEGLLKSVGPNQFNVVSIGFNQPADSP
                     QALKSFATQYGIRQPNWEFLSPPAALVPDLARDFGFVFQASPAGFDHVLQVSILDGEG
                     RIVRQVYGEGVVPSELGEPLKMLLAGQPVESNELIEHLVDRVRILCTVFDPKTGTYKV
                     DYTLPIQIAGGVTFFVLMLIFFINEWRSSRKMARSKGMRNA"
     gene            54788..56368
                     /locus_tag="PK518_00615"
     CDS             54788..56368
                     /locus_tag="PK518_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRM47441.1"
                     /translation="MSLPGSALPVQGPATTPPPLPRLASAYRTLEHGLDMAFGAAANP
                     LKHLGALGFMLLWLLVASGSILYIVLDTSAAGAYQSIAALADVPWMLGTALRGVHRYA
                     ADAFVVVMGMHILREWLLGRYRHWRRAIWWTGVPLVGLAFVSAVGGFWLNWDRLGQYS
                     VVATADWLDALPILGASLARNFLGDVSLSDRLFTLFIFIHIGMPLLMVFGLWFHIQRI
                     ALAKVFPPRPLAWGTFLTLLLLALLLPVHSQGPADLRSQPTALALDWWLLFIHPLVDA
                     TSPTTVWVLLGLAFALLALPPLWPGRAREAVAVVYPEHCSGCSRCFDDCPYSAITMVP
                     HPNAKPGMRLAQVNSDLCASCGICAGSCPSSTPFRSAEQLVTGIDMPQWPVNDLRTQL
                     IQSLHAMPGPERFVVFGCAQGIHSQLQAPDVLHFKLACAGALPPSFVEYALRHGADGV
                     LINGCREGGCEYRLGQRWTAQRLTGEREPHLRRSVPQDRWQVAWTDSGDEHRLAAALD
                     DMRQRSRTATQSVSEESH"
     gene            56368..56871
                     /locus_tag="PK518_00620"
     CDS             56368..56871
                     /locus_tag="PK518_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47442.1"
                     /translation="MKRALNWTGQALLYGGFAVALGVFSRWPVYHPLPEDMAQIKVSF
                     IHHGARVAECRPYTAEEQAKMPPNMRKAEKCERERSPVSIEVDIDGKTVLAHTSPPSG
                     LSRDGASTLYQRLNVSAGQHQIAVRFKDNQKSEQFTSQIDETVTLKPAQVLVIDYSTD
                     KGGIVLQ"
     gene            56868..58307
                     /locus_tag="PK518_00625"
     CDS             56868..58307
                     /locus_tag="PK518_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRM47443.1"
                     /translation="MSSHASPYPDRPATSPVAEPAAAQARANYELSLPNDARLPLARG
                     WLWLGLAALIGSGLFSILLVASRTPYVNQWLPSGNFFHIALVLHVDLSVLVWFVAMAG
                     LLWSLYGRPHASGLGWLALWVTGGGTLAMALAPFLNPGEPVMANYIPVLESPLFLSGL
                     VVFGLGATLLVLRSLLTTPRMGPHLDGQGALGFGLNAGGVATAVALLCFAWSWIVLPT
                     SLHGKAYYEILFWGGGHALQFTWTLLMLVAWLWLANACGARIVLSPRITVLLLLLALA
                     GVFVTPVAYLAHDVTSVEHRNLLTWAMRLGGGPAIVPVALAAVLGVLTVRLSNDAALR
                     PLRAALLSSVLLFAAGGVIGVFIHGSNVRIPAHYHGSIVGVTLALMGAVYRILPALGY
                     QAPQGRMATLQPWLYGMGQLMHIIGLVWSGGYGVQRKVAGTDQVLRSTAEVAGMGLMG
                     LGGLIAIIGGLLFVVVVLRAMRQPQALSH"
     gene            58320..60266
                     /locus_tag="PK518_00630"
     CDS             58320..60266
                     /locus_tag="PK518_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRM47444.1"
                     /translation="MIKKFQDLLQWLFLRMEGLFNAAFGDRINPFYHLGAITFYLFWI
                     VGASGLYLYIFFETGVAEAYSSVIALTTKQWWLGGIMRSVHRYASDAMVLTMMLHMLR
                     YFAFNLYHGFRWFSWVTGVMLIWMVYASGINGYMLPWDQLAQYITVTSFEWLDWLPIF
                     NGSLMRNFLYSDHVGARFFTLLSFMHLGIPLVLLMVMWIHVQRVPKARTTPPGPIVYG
                     LLATLLVLSLIAPVHSQGGQTDLAQAATGLQLDWFYMAVFPLLTEWPLGRVWALVIGV
                     SVVVSLLPWWPPQFKRGDKRLHRVQIHGEQGALSTITVRTDETILDAGLRQGLALPYE
                     CRNGGCGLCVCSIEQGQYEHRPYQKTALPDADKERGKALMCCAVPHEDMAIDVAGFTG
                     ATAEAMETFHASVAQMERLSPDVMRVMLQLPAGQRLLFAAGQYINILLDDGQRRAFSF
                     ANRPGASDQIELHVRLVPGGRWTTHVFDGMKPGDNVRFEGPLGQFTLRESQHPILFIA
                     GATGFAPIKSIVEDAFARGVQRPMRLYWGVRQPQDLYMLQQAEQWQREHDNFTVVPVV
                     SEPTPEDGWSGRTGLVHEAMLADFPSLSGNEVYLCGSVRMVETAVPAFIAHGLDESFC
                     FSDAFVMAAPAPEQQPPGAAAATA"
     gene            complement(60256..60888)
                     /gene="lolA"
                     /locus_tag="PK518_00635"
     CDS             complement(60256..60888)
                     /gene="lolA"
                     /locus_tag="PK518_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HRM47445.1"
                     /translation="MKKAVAAILIAASAQWASADGLKSLENFMKTAHSGRADFTQIVT
                     APPKDGQAQRPKQSSGSFAFQRPGRFKFVYQKPFEQTIVADGQTLWLYDVDLNQVTQR
                     AQAQALGSTPAALLASAPDMAALRADYALEAAPEQDGLQWVQATPKARDGQLKSVRVG
                     FEGDKLAALDILDSFGQRSQIRFTGMQLNPALPAGSFQFKPPAGADLLKP"
     gene            complement(60900..63236)
                     /locus_tag="PK518_00640"
     CDS             complement(60900..63236)
                     /locus_tag="PK518_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRM47446.1"
                     /translation="MTYSLNTLNGAGKSAPRSAAARFGHELGLVLGLLALVFWLLAMV
                     SYSAQDAAWSTSGAGGGRPLANWVGRFGAWLADGSYFLFGFSVWWCVAAGVRAWLSSL
                     ARWMRGGEPVPGATAHGPWLWRAMFWGGLLLLMCTSTALEWSRLYRFEALLPGHAGGM
                     LGYVMGPLAVKWLGFAGSGLAGVVLVVLGAALVFRFSWGHLAERLGARIDALVQTGRA
                     QRERAKDVAVGRRAAREREEVVQEERHESEVQHPQPVRIIEPVLQDAPQSTRVVKERQ
                     KPLFSDMPDSRLPQVDLLDAAQARQETVSPETLEMTSRLIEKKLKDFGVDVTVVAAQP
                     GPVITRYEIEPATGVKGSQIVNLAKDLARSLSLVSIRVIETIPGKNFMALELPNAKRQ
                     TIRLTEILGSQVYHDAKSLLTMGLGKDIVGAPVVADLAKMPHVLVAGTTGSGKSVGIN
                     AMILSLLYKAEARDVRLLMIDPKMLEMSVYEGIPHLLCPVVTDMKQAAHGLNWCVAEM
                     ERRYKLMSKLGVRNLAGYNTKIDEAKAREESIPNPFSLNPEAPEPLERLPHIVVVIDE
                     LADLMMVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFSV
                     GSKIDSRTILDQMGAEALLGMGDMLYMASGTGLPVRVHGAFVSDEEVHRVVSYLKEQG
                     EPDYIEGVLEGGSADGEDSGFGLDDGEGGGEKDPMYDQAVEVVLKDRKASISYVQRKL
                     RIGYNRSARLLEDMEKAGLVSGLTSSGQREVLVPARSE"
     gene            63461..64414
                     /gene="trxB"
                     /locus_tag="PK518_00645"
     CDS             63461..64414
                     /gene="trxB"
                     /locus_tag="PK518_00645"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRM47447.1"
                     /translation="MSTTQHAKVLILGSGPAGYTAAIYAARANLNPVLITGMAQGGQL
                     TTTTEVDNWPADVNGVQGPELMQRFLEHAERFKTQIVFDHINAVDLSRRPFTLTGDSG
                     QYTCDSLIIATGASAKYLGLPSEEAFMGKGVSACATCDGFFYREQEVCVVGGGNTAVE
                     EALYLANIASKVTLVHRRDRFRAEPILVDKLMERVAAGKIVLKTFFTLDEVLGDASGV
                     TGIRIKSTEDGHTEDIKLQGCFIAIGHAPNTEIFQGQITMENGYIATQGGLKGFATQT
                     SVPGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRYLEQQE"
     gene            64565..64798
                     /gene="rpmB"
                     /locus_tag="PK518_00650"
     CDS             64565..64798
                     /gene="rpmB"
                     /locus_tag="PK518_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRM47448.1"
                     /translation="MARVCEVTGKKPMVGNNVSHANNKTKRRFLPNLQYRRFWVESEN
                     RWVRLRVSSAALRLIDKNGIESVLADMRARGQA"
     gene            64819..64989
                     /gene="rpmG"
                     /locus_tag="PK518_00655"
     CDS             64819..64989
                     /gene="rpmG"
                     /locus_tag="PK518_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRM47449.1"
                     /translation="MAAKGGREKIKLVSTAETGHFYTTTKNKKTMPEKMSIIKFDPKA
                     RKHVEYKEAKLK"
     gene            complement(65048..66319)
                     /locus_tag="PK518_00660"
     CDS             complement(65048..66319)
                     /locus_tag="PK518_00660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRM47450.1"
                     /translation="MHPKALLDACAELVRLTLTFEHPADAVVSRYFREHRSLGPRERA
                     TLAETAYTVLRKKPLFEQLARSGQGPRERRLAILGFYGPRDFLKSALSAQEKDWVDAC
                     DAVRPEELLEPQRHNLPQWLAEPLKAQLGDGFWPLVDSLGQSAPLDLRVNALKDKRES
                     VQKELAQAGIKAKATPYSPWGLRIEGKPALTRLDAFTRGAIEVQDEGSQLLALLLDAK
                     RGEMVVDFCAGAGGKTLAIGAAMRSTGRLYAFDVSAHRLDALKPRLARSGLSNVHPAA
                     IAHERDERVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPQAVQELTAKQTAILE
                     SAARLVKPGGRLVYATCSVLPEENEAIAQAFGAAHADFEPLAAGELLARLKVEQAASL
                     CSGGADGQAYLRLWPHLHQTDGFFAAVWQRK"
     gene            complement(66413..66997)
                     /gene="purN"
                     /locus_tag="PK518_00665"
     CDS             complement(66413..66997)
                     /gene="purN"
                     /locus_tag="PK518_00665"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRM47451.1"
                     /translation="MKNIVILISGGGSNMAAMVRAAQQQDWAGRHGIRVAAVVSNKAD
                     AKGLGLAREQGIATQVLDHKAYATREAFDAALAQAIDAYEPALVVLAGFMRILTPGFV
                     DHFAGRLVNIHPSLLPAFTGLHTHQRAIDAGCRFAGCTVHLVTAELDVGPILDQAVVP
                     LLPDDTAESLAARVLTQEHLIYPRAVLAHLLSKI"
     gene            67019..>67115
                     /locus_tag="PK518_00670"
     CDS             67019..>67115
                     /locus_tag="PK518_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HRM47452.1"
                     /translation="MPFDPRQRPLTPAEARVLATLLEKSRTVPDSY"
     assembly_gap    67116..67215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <67216..67866
                     /locus_tag="PK518_00675"
     CDS             <67216..67866
                     /locus_tag="PK518_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HRM47453.1"
                     /translation="ARVLATLLEKSRTVPDSYPLTLNSLAAGCNQKTSREPVMQLADD
                     DLLQALTSLRQQSLVVEIGGARVARWEHNFARGIGVPDQSAVLLGLLMLRGPQTAAEL
                     RLNAERWHRFADTSSVEAFLDELQERSDEKGGPLVMLLPKAPGAREPRWAQLLCGPVD
                     VAALAASQAASAAPAQPALQARVEALEAEVAQLQAQVRRLCAELGLDAPLSPPGQE"
     gene            67913..69094
                     /locus_tag="PK518_00680"
     CDS             67913..69094
                     /locus_tag="PK518_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM47454.1"
                     /translation="MDLAFTPEEQAFRQEVRAWVRANLPPAMAHKVHNALRLTRDDLQ
                     GWARILGKKGWLAFGWPKEFGGPGWTAVQKHLFEEECAMAGAPRIIPFGPVMVAPVIM
                     AYGTPEQQKRFLPGIADGSVWWSQGYSEPGSGSDLASVKTRAERVGDKYIVNGQKTWT
                     TLGQHGDWMFNLVRTSNEGKPQTGISFLLLDMKSPGVTVRPIKLLDGECEVNEVFFDN
                     VEVPADQLIGQENKGWTYAKHLLSHERTNIADVNRAKRELERLKRIARAEGLWDDQRF
                     RDQIALLEVDIVALEMLVLRVLSMEKSGKNPLDIAGLLKIKGSEIQQRYAELMMLAAG
                     PYALPLIQEAMDAGWQGLFPGGTLANAPLASTYFNMRKTTIYGGSNEVQRNIVAQTIL
                     G"
     gene            69109..70224
                     /locus_tag="PK518_00685"
     CDS             69109..70224
                     /locus_tag="PK518_00685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM47455.1"
                     /translation="MDFDFSDDQQQLRDAVRRWVDKGYTFERRRAIVAAGGFERAAYG
                     ELAELGLTALTVPEAHGGLGQGAIDAMVVMEELGRGIVLEPLAQAFVTAGVLAQYAPQ
                     ELQAAWLPRIASGAVLVVLAQQERKARYRLDVCEAKAVVAPNGYAITAIKNIVSAGDQ
                     ADAFIVPAQLDGQIALFLVERAAADVSTRGYGTQDGSRAADLQLASTPATLITTDGLA
                     ALELAVDTAIAAACAYAVGVMDQTCAVTFDYLNQRRQFGVAIASFQALRHRAADMKMQ
                     LELARSMSYYASLKLGAPAEERRRALSRAKVQLGQSMRFVGQQAVQLSGGIGVTDEYI
                     VSHYFKTLTQLEMSWGDTLHHLGEVSARMQEQAGVFA"
BASE COUNT        11900 a        23523 c        22490 g        12111 t
ORIGIN      
        1 ggcggccctt gcacggcggc cctcgcccgg gtcgcgccag tttcataggc tgcggcgtaa
       61 tgcgacgcag cttggcaaat tcatacaaag gaacacccat gtcctggctc gaaaaactcc
      121 tgccttccaa aatccagcag accaacgcca ccgagcgtcg ccagatgccc gagggcctgt
      181 ggaccaagtg ccccagttgc gagaccgtgc tctacaagag cgacctggag cagaaccaga
      241 acgtctgccc caagtgcagc caccaccacc gcatcggcgc gcgcgcgcgg ctcaatgcct
      301 ttctcgacgg cgagggccgc tacgagatcg gccaggaggt gctgcccgtc gatgcgctca
      361 agttcaagga cagccgcaag taccccgagc gcctgaagga ggcgctggag aacaccggcg
      421 agaccgacgc gctcatcgtc atgggcggcg cggtcaagag catcaacctg gtcgccgcct
      481 gcttcgagtt cgacttcatg ggcggctcca tgggcagcgt ggtcggcgag cgcttcgtgc
      541 gcggcgtcga gaccgccatc gagcagaagg tgcccttcat ctgctttacc gccaccggcg
      601 gcgcgcgcat gcaggagggc ctgctgtcgc tgatgcagat ggccaagacc aacgccgccc
      661 tcacgcgcct ggccaagaag ggcctgccct atatcagcgt gctaaccgac ccgacgatgg
      721 gcggcgtctc ggccggcttt gcctttgttg gcgacatcgt gattgccgag cccaaagcgc
      781 tgatcggctt tgccggcccg cgcgtcatcg aatccaccgt gcgcgtgacc ctgcccgagg
      841 gctttcagcg cgccgaattc ctgcagacca agggcgcggt ggacttcatc tgcgaccgcc
      901 gcgaactgcg cgagaccgtg gcgcaatgcc tgtccatgct gcagcgcctg cctgccgacg
      961 ccgtagcctg atacaaaaat aatagctgtc agcgctttcc cagcaagcgc tagaagctat
     1021 tttgttcaag tttattggcc catcaggacg ctggcctgca aatggcccag catgatggac
     1081 agcacctgca gcagcaccag cagcaccagc ggtgatagat caatgccacc cagcagcggc
     1141 acgacgcgcc gcaccggttg cagcagcggc tcgctcaggc gggcaatgac gtcgctgatg
     1201 ggtgtgcgcg tctgcatcca cgacaagatg gcataggcaa tcagcaggcc catcaggccc
     1261 gacacggtga cgcgcgccag actgaacagc gccatccacg gcagccaggc caggcccagg
     1321 cctgccccca ccagcaacca cagaagcagg tactgcacca gctgcagcaa aaaggccgca
     1381 accaggcaag acaaatccca gcgccccgca gccggtatca gcttgcgcag cggcagcacc
     1441 agccagtcgg tgagagcaaa gaccagccgc cccaccgggt tgccaaaagg cacgcgctgc
     1501 aactgcatgt acagacgcag cagacaggcg ccggtcagca gcccgctcac gacttcaaga
     1561 agataggaaa tgatctgaaa cagcatgggg atcgacaaat gcatgcgccc aacaagcgcc
     1621 acggcgccga cggtggcgca gcgtgggatg atagcgaggc tgcctgggcc caacgccatg
     1681 ccggatcccc tgacattgac atcgctcccg ctgttcccgc taaacaccgt gttgtttccg
     1741 ggcggcttgc tggcgctgcg cgtgttcgag gtacgttact tggacatggt gcgcaaatgc
     1801 cgacagaccg gctctccctt tggcgtggtc acgctcacag cagggcagga ggtgcgcagc
     1861 gccgcagctc ccgaggagcg gttccagccc cagggcacac tggccgtcat cgaacagctg
     1921 cagacgccgc aacccggcct gatcaccctg cactgccgcg gtaccgagcg ctttcacatc
     1981 acgcagcaac accagctgcc ctacggcctg tggctggcag acgtacagca attgcccgac
     2041 gacccgcagg taccgctgcc cgccgacttg caaagccttg cggccaagct ggggctgctg
     2101 ctggcagcgc agcatcagcg ccgcagcacg cctggccacc ccattccccc cacgcctgcg
     2161 caactgcagg actgcggctg ggtcgccaac cgctggtgcg agctgctgcc cgtgccgctg
     2221 gaactgaaac accacctgat gacgctggac agcccgctgt tgcgcctgga gctggtgggc
     2281 gatgtgctgg agcgcctggg aattgcaccg tgaacatggc gcgatcgagc acccacacac
     2341 acggcggctg ggtggttcct gccatacccg gccggccggt caacccagcc ccggacacct
     2401 ggcatgtgcg aaaaccctaa aatcgccggt ttgccccttc ttgcccgacg gcaccatgtc
     2461 tgaacaaaac caagccccca tccagcccca ggacgaaaac cagctcatcg ccgaacgccg
     2521 cgagaagctc aaggcactgc gcgcgggcca ggcccagggc cggggtgtgg cgtttcccaa
     2581 tgacttcaag ccgcgggacc acgccgccag cctgcagcag cgctacggca gcctcgctgc
     2641 cgaggcgctg gagacggaga acatatcggt cagcctgggc ggccgcatga tgctcaagcg
     2701 cgtcatgggc aaggccagct ttgccacgct gcaggacggt tcgctgggtg acacgggcgg
     2761 tcgcatacag ctgtatgtga cacgcgatgc cgtcggcgaa gaggtctacg ccgacttcaa
     2821 acgctgggat ctgggcgaca tcgtgggcgc ggagggccag ctcatgaaga ccaagaccgg
     2881 cgagctgtcg gtcaaggtga ccaccttgcg cctgctcacc aagagcctgc gccccatgcc
     2941 cgacaaattc catggcgtgg ccgaccagga ggtcaagtac cgccagcgct atgtcgatct
     3001 gatgaccgac gacgccgcgc gccggcgctt cgtggcgcgc agcaaggcgg tcggctcgat
     3061 ccgcaacttc atggtcgagc atggcttcct ggaggtggag acgcccatgc tgcaccccat
     3121 cccgggcggc gccaacgccc gccccttcgt tacacaccac aacgcgctgg agcaggagat
     3181 gttcctgcgc atcgcccccg agctgtactt gaagcgcctg gtggtcggcg gcttcgagcg
     3241 cgtgttcgag atcaaccgca acttccgcaa cgaaggtatc tcggtacgcc acaaccccga
     3301 gttcacgatg atggagttct acgcggccta ctggaactac cgcgacctga tggacttcac
     3361 ggaacagctg gtacgcgacg cggcgctcaa ggccacgggt acgctgcaac tcacctatgg
     3421 cgggcgcgcc gtcgatctgt cccaaccctt ccagcgcctg acgattcgcg aggccatcta
     3481 ccagtacacc gaggcaggcg cccatgtgga cgatgccgcc tggctcatcg gcgcgctgaa
     3541 aaagctcggc atgagcgagg aaaaggatcg cctgtccacc cgcagcctgg ccagcctgca
     3601 ggtgctgtac ttcgaggaaa cggtggagga caagctctgg cagccgacct tcatcatgga
     3661 acacccgacc gagatcagcc cgctggcgcg cgccaatgat gcccgccccg aggtcaccga
     3721 gcgcttcgag ctctacatca ccggccgcga gttcggcaac ggcttcagtg agctgaatga
     3781 cgccgaggac caggccgcac gcttcaacgc ccaggtggct gccaaggaca gcggcgacga
     3841 cgaggccatg ttcttcgacc acgacttcgt gcgcgcgctc gaatacggca tgccccccac
     3901 gggcggctgc ggcatcggca tagaccggct gatgatgctg ctcaccgaca gccccagcat
     3961 ccgcgacgtg atcctgttcc cggcattgcg ccgcgagcaa taagccagga cccatgctgg
     4021 agcgcctgaa gaccctgctg ccgccatgga tgcacgactg gctggacatc atcgtgcccg
     4081 gcgtgcagat cgcgctcatc gtgctggcag cgctgctgct gcagcatctg gtgcgccgca
     4141 tcatccggcg cgcaagcgac cactaccagt ttccgcacga gctcctcacc cccatcaacg
     4201 gcgtggtgcg ctggctcatc ctgggcggcg ccggcctgct ggcgctggag cgcctggggg
     4261 tttcggcagc ggtgctgtgg acggccttca ccagcttcgc cgccgtcggc gccgtggcct
     4321 tcttcgcggc gtggagcgtg ctgtcgaacc tgttctgtgc cttcctgatc ttcaccgtgg
     4381 ggccgtttcg cgtgggcgac catatcgagc tgctggacac ggcggagaag cccggcgccc
     4441 tgggccgcgt ggtggacatc aacctgctgt acaccacgct ggaagacgcc acggcaccag
     4501 agcccggctc actgctgcag atccccaatg cactgatatt ccagcgcgtg ctgcgccgct
     4561 ggccagcggg cgttccagtg ccgcagggca agctgcgccc tgtgccgccc agtgggggtg
     4621 cggcaaccac ggaatctgcc gcggcaccca agggtgctag ttttccctga ggcttccagc
     4681 ttccgcaggc gcaaacagcc tgtcacaatc ggcgccgccg tagcaaaaaa atggcagcag
     4741 cgccagcctt ggtttccctg ttgtcaacaa aatgcgcatg gtgaatcatg gcggtcttga
     4801 tcgcaggccc ctacccgggg cctgatgatt tttcagaccg ctttacccaa agttggagat
     4861 cgttttatga gcattccgtt tcgcatgcat tccgtcgtcg ccgcactggg cctggccagc
     4921 gccgctttgt tgtccaccgc gcatgcgcag gtcaagatcg ccatggtgat cccggccacc
     4981 ggcccggtca cccagtacgg tgacatgatc aaggaaggcg tgaacaccgc cgtggagcag
     5041 gccaacgccg ccggcggcat caacggcaag aagatcgaac tcgtcaccgt ggacgatgcc
     5101 tgcgaaccca agcagggccc cgttgccgcc aaccgcgtga tcaacgacaa gatccactac
     5161 gtggtcggcc ccgtgtgctc gggcgcagcc attgccgcag cgcccatcta caacaacgaa
     5221 ggcgtggtcg tcgtgactcc gtccgccacc tcgcccgcgc tgaccgaggg caagaacttc
     5281 cacttcatct tccgtaccat cggccgtgac gaccagcaag gccccgccgc cgccaagttc
     5341 atcgccgaga agatcaagcc caagaaggtc gccatcctgc acgacaagca gtcctacggc
     5401 cagggcattg ccgcctcggt gcgcgacgac ctgaagaagg ccggcgtgaa cgtagcgctg
     5461 ttcgagggca tcaacgccgg cgacagcgac tactctgccg tcatcaccaa gctcaagagc
     5521 gcgggcgtgg acttcgtcta ctacggcggc taccaccccg aaatgggtct gctgctgcgc
     5581 caggcaggcg agcagggcct gaaggtcaag ctgatgggcc ccgagggcgt gggcaacccc
     5641 gaggtcaacg ccatcgccgg tccggccgtc gaaggcatgt tgctgacgct gcccgccgac
     5701 ttctccacca acccgaagaa cgccgccatc gtcaaggcct tcaaggacaa gaagcgcgac
     5761 gccggtggcg ccttccagat gacggcgtac gccgctgcgc aagtcatcat cgacagcatc
     5821 aaggccgtgg gcgacaaccc cgccaaggtg gccgatcaca tgcacaaggc cacctttgag
     5881 acgcccctgg gccccatcag ctggaacaag agcggcgacc tgaagtcctt cgacttccag
     5941 atcttcgaat ggcacaagga cggcagcaag tccgtcgcca agtaagtcct tagtcgctga
     6001 caccccgacg cccttccctc caggagccag gcgatcgggg tttttgccgt tttctcctga
     6061 gtctgtgaaa aaatctcccg tgctcgttct gagcgcgggg gcttttcaca gcccctgcac
     6121 ggcagtcttt tttgttccca tctctttatc gagaggccca ggatgtctga attcctgccg
     6181 cagctgatac agcagctctt caacggcctg tcgctcggag cgatctacgc cttgattgcc
     6241 attggctaca ccatggtgta cggcatcatc ggcatgatca acttcgcgca cggcgagatt
     6301 tacatgatcg gcgcctacgc cggcctggtc accctggccg ccgtgggcac acaaagtggc
     6361 ctgccggtgg ccgtgctgat tgccctgatg ttgttggtgg ccgtgctggt cacgggcacc
     6421 tacggcttcg tcgtcgagca gatcgcgtac aagccactgc gcaatggccc gcgcctggtg
     6481 gccctgatct cggccattgg catgtccatc ttcctgcaaa actgggtggc cctgggccag
     6541 ggcgcgcgcg acatggccgt gcccgccctg ctgcccggtg cgctgcgctt tggcgacgaa
     6601 ggcggcttcg agatcttcgt gccctatgcc cgcgtgctga tcatcgccgt caccgtggtg
     6661 ctgatgatcg ccctcacgct ctacatcagg cattcgcgca tgggccgggc atcacgcgcc
     6721 tgttcgcagg acatgcacat ggcgggcctg ctgggcattg acgtgaaccg cgtgatctcc
     6781 ttcaccttca tcctgggcgc ggtgctggcc gccgtcggcg gcgtgctgat cgcgttggca
     6841 gtgggcaagc tcaacccgtt catcggcttc atcgtcggcg tgaaggcctt caccgcggcc
     6901 gtgctgggcg gcatcggctc aattcccggc gccatgctgg gcggagtgct gctgggcatc
     6961 gccgagactt ttgccgccgc ctatatttct tcggaataca aggacatcgt ggcctttggc
     7021 ctgctggtgc tgatcctgct gttccgcccc acgggcctgc tcggcaaacc tgaagtggag
     7081 aaagtctgat gagcgccgct atcacctcct ccgcccgccc gagctggcgc cagaacctgg
     7141 gccacgccct taccgcgacg gtgctcaccg ccgtagtggt tacgccgatt ttcggcctgc
     7201 acctggaacg cgcgggtgtg cgcaccatca tcacgcagca ctggagcaac gtggcctggg
     7261 cctgtttgct ggtgttcatc gtccagatgc tgcgccccat gctggccggc agcggggtgc
     7321 gcctgccctg gcccaaactt ccagccattc cggcgcgcca gcgtggcgtg tggatcgcgg
     7381 cggcactgct gctggcggtc atctggccct tctttggcgg ccgcaatgcc gtggacattg
     7441 ccaccctggt catgatctac gtgatgctgg gcctgggcct gaacgtcgtc gtgggctttg
     7501 ccggcctgct ggatttgggc tttgtcggct tttacgccgt gggcgcctac acctacgccc
     7561 tgctgttcca ctgggccggc tggagcttct gggaggcgct gccgctggcc ggtgccgcgt
     7621 cggcgctgtt cggctttgtg ctcggctttc cggtactgcg cctgcgcggt gactacctgg
     7681 ccatcgtgac cctgggcttt ggcgaaatca tccgcctgct gctggtcaac ctgacggact
     7741 tcaccggcgg gccggacggc atctccagca ttcccaagcc cacggtgttc ggtctggaac
     7801 tggcgcgcag cccctcggta gagggcgcca ccaccttcca ccagtttttc gggctggaat
     7861 tcaactccat gcacatggtg atcttcctgt acctgatggc actcacgctg gccgtagtca
     7921 cgctgtggat ctccaaccgc ctgatccgca tgcccattgg ccgcgcctgg gaggcgctgc
     7981 gcgaggacga gatcgcctgc cgatccctgg gcctgaaccc gatgaagatc aagctctcgg
     8041 cgttcacgct gggcgccatg ttcgcgggct ttggcggcgc cttctttgcc gcgcgccagg
     8101 gcatcgtgaa ccccgagtcg ttcaccttca tcgaatcggc gctgattctg gccatcgtcg
     8161 tgctgggcgg catgggctcg cagctgggcg tgatcctggc tgccatcttg ctgaccgcac
     8221 tgcccgagct ggcgcgcgag tttgccgagt accgcatgct gatcttcggc ctggtgatga
     8281 ttctgatgat ggtgtggcgc ccgcaaggcc tgctgcccat gcaacgccac cacgtggagg
     8341 tgaagtgatg gccggtagcg acaaaaatag tttcttgcag atcgaaggcc tgagcatgcg
     8401 ctttggcggt ctgctggccg tcgatggcat cgccttcgac gtcaaggaac gcgagatctt
     8461 cgccatcatc ggccccaacg gcgcgggcaa gaccacggtg ttcaactgca tcagcggctt
     8521 ttaccgcccg accacgggcg ccatccgcct gcagggacgg gacatcgccg gccagggcag
     8581 ccacagcgtg gcgctgcacg gcctggtgcg aaccttccag aacgtgcgcc tgttcaagca
     8641 catgacggcg ctggaaaacc tgctggtggc ccagcaccgc catctgtcca ccaacctgct
     8701 caccggcctg ttcaagaccc gcggctatcg cgaaagcgaa gaaagggcct tgcagaacgc
     8761 actgttctgg ctggactaca tgggcctgcg cgaatttgcc aaccgcgagg ccggcaacct
     8821 ggcctacggc caccagcggc gtctggaaat cgcccgctgc atggtgaccc agccgcgtgt
     8881 actgatgctc gacgagccgg ccgccggcct gaacccgcag gaaaagaagg acttgcagag
     8941 cctgatcgaa aagctgcgcc aggaatacgg cgtggccgtg ctgctcatcg agcacgacat
     9001 gagcctggtg atgggcgtgt ccgagcgcat cctggtgatg gaatatggcc gcccgattgc
     9061 gttgggcacg ccagaggcca tccagaagga tgaacgcgtg atcaaggcct acctgggaga
     9121 agaatgatgc aagccatgct ggaactgaag ggcatcagca cccactacgg cgccatctgc
     9181 gccgtgaaca acgtgagcct gcacgtcaac cagggcgaga tcgtctccct catcggctcc
     9241 aacggcgcgg gcaagacctc gctgctgatg acgctgtgcg gcaacccgcg cgccagcagc
     9301 ggccaggtgc tgtacgaggg agaggacatc accaacgccc cctcgcatct gatcatgcgc
     9361 aagggcatcg ccatctcgcc cgaggggcga cgcatcttcc cggcgctcac cgtggtcgaa
     9421 aacctgcaga tgggcggctt cttccagtcc aaggacgcga tcgccgaggg catggaatac
     9481 gccttcaagc tgtttccgcg cctgaaggac cgcgccaacc agcgcgccgg caccatgagc
     9541 ggcggcgagc agcagatgct ggccattggc cgcgcgttga tgagcaagcc acgcctgctg
     9601 ctgctcgacg agcccacgct gggcctggcg ccgctgatca ttgcgcagat cttcgagatc
     9661 atccaggcca tccgcgccca gggcgtgacg gtattcctgg tggagcagaa cgccaaccgg
     9721 gcgctgtcga ttgccgaccg cggctatgtg ctggagacgg gccgcctggt gctggaagac
     9781 acgggcgcca atctgctgat caacccggaa gtgcgcaagg cgtatctggg gcactgagga
     9841 ctcaccacaa gcgcccccaa agcggccttt ggccgcttta tttatttcta ggccaaagcg
     9901 cttaccagat aagcgcttga tgctataaaa ataaaagtaa cttcggcact tggagcatcc
     9961 tcaacgcggc cccggaaagt ctctccaagt gctcagaacc tgtctcgtgg gacaggctgc
    10021 tgaacagagg ctcgcatccg ccaccggggc gaggtcaccg tccgtcagtg tgcctccccc
    10081 gcctccggca cataaaccat cgacccgtcc ttgagccgat aggccacgcc ggccttggcg
    10141 ccctggcctt cgccgatctc gccgctggcc ttgtagtggt cgaccttctg gccgtccttg
    10201 acgatgattt ccatgttgtc cttgcccggg ttcttgatga tggtcaccga ctcggtgctg
    10261 aacgggttga ccgagctgtt ggccacggcc agcagcagcg cggcgatgac gaccaggaac
    10321 acgtcgatca ggttgacgac cgagaggatg gggtcgtccg cctccaggtc ttccatcagg
    10381 cgcaggcggc tcatcgcgca ccctccaggg ccatgacggc caccggatca tggtcgcgct
    10441 ggggctgggg cgcgtcatgc tctatcgccg ccagatccac ggcgtaccag cgccggcgca
    10501 cgttgaccac ggtgtagctg atcgacgccg ccaagagcgc caggatcacc gccgaaaacg
    10561 ccaccgtgag gttgcgcgac acgtcggcca tctggccgtc ggacagcgcc atcagcgccg
    10621 ggcccatggg gatcatggtg gccaccaggc ccagcatggg cgcgatgcgt gtggcgatgc
    10681 gcgcgaactc catgcgcttg gtggccaggg cttcgagctc ggtcagcgtc atttgggggt
    10741 tgcgcgcgag cgctgccgcc agctcaaagc cgccggcctg gccgttgtgg cgctgccagg
    10801 cctgccagat gaaggcgccc agggcgtaga aggcgtgcac gaacaacagg ccgatgagga
    10861 tgagcaccgg cgtcagaaac agctggctgg cggagtacat cgccaattcg atgggatttg
    10921 cggtcatggt ggggggggta agtggtaagt ggtaagtggt aannnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nntcgatggg atttgcggtc atggtggggg gggtaagtgg
    11101 taagtggtaa gtggtaaatg gaaagtggtg agtcgtagat agtgcgtggt gcgtagcctt
    11161 actccctccc cctctggggg agggttgggg tgggggccag cggcgctgat aagtgaaagg
    11221 gagatgcgct agccggcagc ccccatcccc accttccccc agagggggaa ggggccagag
    11281 cggcggcgct gttgccaggc accggcacaa ctcacgccag ccagcgcaac ggcgaacagc
    11341 agcgtggcgg ggttgaaggg cggcagctcc ttgggctgga ccttttgcat gcgcatgccg
    11401 gtgaccatgg gcggcggcgg tggctctggc ggcgcggctt cgggctgggc ctcggcctgc
    11461 tcctggggct gcgctgcagc ccgggccggc gcagcggctg cggccgtggc ctgcaggcca
    11521 aagccggcct gggccgcgcc ctcggcgaca aactcggaga agcgcgcgtt gtcgctctgc
    11581 acgtcgtagc gctcggacag gtcgcgccag cgctccttca gctccttcac cgtggcggcg
    11641 tcggccttcc agtagccctg gcgcgcggct tcgagcatgc gctccatggt ctgcgccagc
    11701 gcgtgcgggt tcttggactc gaaccatttc ttcaggccca gcttgtgctt gtcgcgcaca
    11761 tagacgtcga ccatctcctg ccactggtcg tcgcgcacga tctcgcgtgc ggtggccgtc
    11821 cagccccaaa aattgttggt cgcgtccagc acctgcagcg tgccggcgta gccctccttc
    11881 atcaggccct ggatgtagcc ggggtggaac tggcgcgtgg ccagctcctt ggccaggaac
    11941 tgcgccgcgc cctcgacctt gcccgagccg ctgccgcgca ggttgctgat gtacagttcg
    12001 ggcgccttgc cgtccaggtg acgcacggcc atggcgatgc cgcccaggta ctggaagggg
    12061 tcgtcggtgg tcagcatgcc gtagaggttg gagctgcgcg agagcaccgc gccctcggtg
    12121 ccgctcaggt gctcggcgta caggttcacg cccttgccgc ccttgccgcc cttgccacct
    12181 ttgccgtcac ctacgcccgc cacaccggcc tggccccatt ccttctcgtc cgggccgtag
    12241 gcgaactgca tcttcgacag atacagctcg gccatcttgc ggtcgccctc tttcttgccc
    12301 ttccaggtgt cagtggcctg cgtggcctcg tccaggccgg tgccgtagcg cccgctctca
    12361 gaggcaaaaa tgcgcgtggc gccggccatc tcggccgcct tggcgtcggc cccctgggcg
    12421 atgagctttt gcgcgatgcg ctgggcgttc tgcgccaccg ggttatctat ctctgtagca
    12481 cgagacgcaa gcaacacggc ctgtgcaagc tgtttcatca cattggggaa atggtcgcgg
    12541 tacaggccgg tggccgacag caccacgtcc acgcgcgggc ggccgagctg ctcgcgcggc
    12601 accagtttca cgccggtgac gcgcccgccc ttgtcccaca ccggctccac acccaagagc
    12661 cacagcgcct gggcctcgag catgccgaag tggcgcatgg tctcgaccga ccagagcgtg
    12721 aaggtcagct tggacggctg cttgccgtgc agcttccggt gctcggccag caggttgtcg
    12781 gccgccagct tgccggcctc ccaggcctgc ttggtgggca cgcgcgaggg gtcgaagccg
    12841 tagaggttgc ggccggtggg gtaggcatcg gggttcttga tcgggtcgcc gccgtaggag
    12901 gtgggcaggt agcggccggc cagcacatcg agcaggccct tcaactcgcc ctgcgcaccc
    12961 aggttgtcgt aggccgtttg ggcctgcgcc agggcttgct gcaggtcggc gggcaggccg
    13021 gtcatgggtt caccggccac cacatgcttt tgcaagagct ggtacggcac ggtctgcgcc
    13081 agcttgtccc aggggccgac cagggcctca tccagatcgg ccagcggcac accggcgcgg
    13141 cgcgcggcgg cttcccagaa atttttgccc agcgtgagca gcaccgtgcc caggcggtgc
    13201 agctcctctg gcgcctggcc caggcggtgc aggccctggg gctgggcggt ttgcgccagc
    13261 tcgtgcaggt ggttgtgcag ttcatgaaca aagccggcaa agtcagtttt cacccgcgcc
    13321 tcggtccagc ccatgtcggc aatcacgcgc tccttgcgcg cggcggccag caggtcggcg
    13381 gccatgcgat ctcgcaccgc gccttcgtcc tgcgcctgcc actggtgcag caggtcgtgc
    13441 atttgcgtca gcgcgtcgtg cagccccccg ggggcaaagg gcggcgtctg gtggctgatg
    13501 atggtggcgc gcccacggcg cttggcctgc gtagcttcgc caatgttgtc ggcgatatac
    13561 gggtaggcga cggggatgtc gcccagcgcc agcagcggcg cgtcatgcac cgacaggccg
    13621 cgctccttgc ccggcagcca ttcctgcgtg ccgtgcgtgc caaagtgcac cagcgcgtcg
    13681 ttcttctgac gcgtccacag ataggtggcc aggtagtggt gcggcggcag gtcaacggtg
    13741 gagtggtaga tctccttctc tttggcgcgc gcgtcagtgc tgccgggggc cgctccgctg
    13801 cggccaggct ggggcagcag tgtcaccttg cccagctgca ggcgcgggat gatgaagtag
    13861 gcctggccgt tctggcgcag cacccagggg gatttttcgg gatcacccca agcgcggcgc
    13921 agctcgtcct gcgtggcctg gggcaggctg gccagccagg cgcggtagtc cttcaccggc
    13981 agcaactcag ccaggccgtc gcgcaacagc ggctccagca gccccgggcg gtagctgggc
    14041 gccagcaggc gctgcagcag cgtgatgagc atggcctcgt cgggcgattc ggtctggtag
    14101 ccggcgcgca ccatcgcggc cagggtgttt tgcaggctgc gcggcacgtt caaaaacgac
    14161 gcgctcaggt tcttctcgcc ggcggggtag ttccagaaca tcacggcgac gttcttgtcg
    14221 gcattgggcg tgcgctgcag cttgaccagg ttgagtgcct tggcggccac ggcggcggcc
    14281 tgcgcggcta tcggcatgat ctgctcgtcg cccttgcgca cggcggacgc gacctggatg
    14341 tcggtcacgc cggcgtattc ggcctgcgcc aggtagaacg gcacgtccat cgccgccacg
    14401 ccctgccggc tggcggccca atccgcttcg tcgccacggc ggtgcgtcat ggcctggatg
    14461 accgggaacc ctaagcgctc gaattcttcg cggcgctcgt cggcgttcag catgatctgc
    14521 gtgttgatca gcacgtcggc cagcacgggc gtgccggggc cgccgggctg cagcaccttg
    14581 gcaaacccgc catcgcccat catgggcgtg tagaacggca gcgccacggc gccgccggcc
    14641 tcgatgcgcg ccaccatgtc gtcgatgtag gcggtctgca tcgagcctat gtattgctgg
    14701 tgcagcgaaa tggccaccac cggcttgcgc tgcgcggcct tcatttccac accctggcca
    14761 cgcagaaagg ccacggggtc ggccatgacg gtgccgggca ggcgcgggtg gtagatgccg
    14821 gtcttgggga agaccaccgg gtcgggcgcc gccgtcaggg cgttgccgcc cttgagctgc
    14881 gcagccagtg tggcgaagaa accctcgtaa ttctgccgcc cgccgctgcc gtagtaggca
    14941 atcaggcgcc gcgccacggc ctcgggcagg cccccgcccc aggccggctg ctggtcgtac
    15001 agccaggcgt tgggtgcctt cagggccggc agcgcgcgcg tcaggcgcgc acgcacctcg
    15061 tcttgcagat agctgtcgaa gaacaccgcg tcgcgtccgg cccacaggcc ggcgtccaca
    15121 tcggccggca ggctgttgat gtagcgcacc tcgacgtcga tgccgtgcgg ccgtgcaatc
    15181 tcctgcagct ggtggaactt gccctgcggc acgttgccgg tggcgacgaa cagcaccgac
    15241 gcggcgcgcg ccgcgccgcc cagcagcagc aacgcggtgc acagcagcca gcgcagcttt
    15301 tggttttgaa acaaattggc ctcctgcgcc ctatttatga gcgccagcag ctactatttt
    15361 tatagctaac aaatcagaaa tccagacgtg ccgaaacaaa gacccggcgg cccagctcgg
    15421 cgtagccgaa gttgggcgac ttctcggcca ggcgcacatt gcccaggttc tccaggccgg
    15481 cgcgaagatg aaggttttgc gtggcattga gcttcatggc gtagcccgcg ctgacgttga
    15541 acagggtgta ggccggcagc ttgttgccgt agttctcctg gctgccggtg tattgcaggc
    15601 ccatgcgggc gtcccagccg ccgcgcttcc aggtcaggtg cgaggccacg ctggtcgtgg
    15661 gccggtcgtt caggcgcttg cccgtggcct tgtcgcgcgt gtgcagcaag gtggcatcgc
    15721 tgccccagga cagctgcctg gagagctgca gggtgaagcc cgcctccagc ccctggatgc
    15781 gcgcggcgtc gatgttgtcg tactggtaga cgcggcgtat gccgatctgc tgcagcagct
    15841 tgtaggtgat caggtccttg acctcggtgt ggaaggccgt ggcgcgcagc gacagcgcgc
    15901 tgctggcctg ccagtcggca ccgatctcca gcgagttgga cgtctcgggc ttgacgttgg
    15961 cgttgcccag gaagctgtgc ggcccctcgg ccccgacgta gttgggcgag atctgcttga
    16021 gcgtcggcgc cttgaaggcg tggccccagc cacccttgac gacgagctgc gggctggcct
    16081 cccagaccag gtaggcgcgc gggctcagct cggagccaaa caggccatgg tggtcggcgc
    16141 gcaggcccag ggtggccatc agattcgcgc ccagcgagaa ctcgtcctgc acgaacagcg
    16201 ccttgtgcgt cacgtcgtca cggccgccct tcaggccggc gttgaccagc tcttcgctcc
    16261 gccattcgcc gcccagggcg atgttgtggc tgccgcggcg cagcgtgaca tggccgtcaa
    16321 cgacatcgtc ctgcatgtcc tgcgggcgcg tcggcgccac gccgttgctg cggctgttga
    16381 tgacgctcat ctcgctgcgg taggcgcgca gctgggcctt caggtcacca aagtcgcccg
    16441 tccagccgac atgggtctgg cgccgatcca ggtcgtagcg gttgacatag gcgcggccgg
    16501 cggtgttcac gtcgtcgtac aggcgccgtt cctggccgtc gctgatgccg gcctgcaggc
    16561 gctggctggc cgtcaggtcg aactcggcgt tcacgcccag gttgcgcggc ctgcggcctt
    16621 cgacctcgct gtagcgcagg tcatcaatgt ctggcgtggg gttgctgcgc gcgtactcgg
    16681 cgttgaccga caggcgcgca cgctggccca gcgggcccgc ggcgtagacc gaactgccga
    16741 actggctggc cgcgtcgctc ttgaggccgg tgccgccgga gacaccgatg gagccgctcc
    16801 agtgatcctt gggcttcttg ctgatgacgt tgatcacccc gcccagcgcc tcggagccat
    16861 acagcgccga catcgggccg cggatgatct cgatgcgctc gatggccgag attggcagcc
    16921 agccgtactg gtagtcggag tggccgatca cgtcgtcgct ggcgctgatg cgccggccgt
    16981 cgatcagcgt cagcgtgtgc ttgccctcca ggccgcgcag cgccagcgtc ttgcgcccgc
    17041 ccacctggcg cccctgcagg gtgatgccgg gcgcgccgcg cacggcgtcc agcaggtcgc
    17101 cggcgttctt ctcggccagt tcctcggcgc tgatgacggt gacgctggcc ggcgcggtgc
    17161 gcgcgtcctg ctcggtcatg gtggcggtga cggtgacggc gggcaggccc tgggcgggcg
    17221 ccagggccag ggtctcggtc tgcgcctggg cggcgccggt cgccagcagc agggcggcca
    17281 gggccagcgg atggcggcgg cagggtacgg caaatgggat acgcatgaac gtccagaagt
    17341 aagaaccgca gtggctggac gctggcgccg caagggtgcg cgtggggctg ggcggaaagt
    17401 tgaaaaaacc cgatatcgac gccaagcacc agaccggtca gcagagtgtt ttgcccccgg
    17461 gacttgcctc ctggagtggc gaacacggca cccacaggcg gtggcagcgc gaaactgggc
    17521 ggaagtgtaa acgcaagtcg ttcgcattcg catcaatatt taaagaaaat gcggccgaat
    17581 gatctttgtc aataacgccc aggcgcgctg cagcccgcct gggctgcatg gagcccccat
    17641 gggcgcagat aatgcgcatc accatgcctc tcttgcgcaa cgctccccgc ctggcccgcc
    17701 tgctgctggc ctgctttgcg ctgtcgctcg cggtggccat ggccgcgccg ctggtgcagg
    17761 cccggcccat ggaacgcgtg tgcactgcat cgggcgatga acgctgggtg gcggccggcg
    17821 gcaaccaggt cggcaccctg ccctatgagg gccaccagtt cagctgcgcg ctgtgcctgc
    17881 cgcccatgct gcccggcgcg caagcggcca cccttcccca gccccagggc cagcccttca
    17941 tggcccaggc actacgcgtg cgcatcggcc atgtgccggc actcagccgc gccgcctttc
    18001 cgccacgcgc cccgccggcg cccgcgccat cgccttgcaa tctttctgag tgcggcggcg
    18061 ccatctgcgc cccgccccga tgcgcgcatg ggccgggatg acccatcgcg ctcgccctcc
    18121 accgatccaa tcaagagact ttttatgaac ttccaaccca ttgccacccg tatcaccctc
    18181 gccgccgccc tgctgctgac cggcttcgcc catgcccagg tcagcgtgca ggacgcctgg
    18241 gtgcgcgcca ccgtgccgca gcaaaaggcc accggcgcct tcatgcgcct gaccgctgcc
    18301 caggacatgc gcctagtcgc cgccagcagc cccgtggccg gcattaccga ggtgcacgag
    18361 atgaagatgg tggacaacgt gatgaagatg cgcgccgtgc cggccctgga tctgccggcc
    18421 ggccaggccg tggagctcaa gcccggcggc taccacatca tgctgctgga cctgaagcag
    18481 cccgtggccc agggcgccag cgtgcctctg accctggtct tcgagggcaa ggacggccag
    18541 cgccagaccc aggagctgca ggcccccgtg cgcgccctgg gcgccacggc ggcacccgcc
    18601 atggggcatg gcaagcacag cgggcactga tattttgata cttttatggc tgtagcgctt
    18661 taccaataag cgcctaaagc tataaaatat atagcatcaa agacacaggc cggacgcctg
    18721 ccggcagagg cttgacccat aatcaagcgc ttttgcgtgc ggcccgctgc gcacgccctc
    18781 ctctgccacc accatgcacc ctgacgccca acatctctgg cgcggcccgc gctgggccct
    18841 ggccatcctg ctggcattgc tgggcatggt ggggccgttc tccatagaca cctatctgcc
    18901 ggcctttccg gccatcagcc aggcgcttgg cgccacgccg gtgcagatgc agcaaacgct
    18961 gtcggcctat ctgtttgcct ttgccttcat ggcgctattc catggcgcgc tgtctgacag
    19021 ccttggccgc cggcccgtgg tgctgtgggg gctggcgctg ttcacgctgg cctcggccgg
    19081 ctgcgcgctg gcgcagacca cggggcagct ggtggcgttc cgcgcgctgc aggggctgtc
    19141 ggcgggtgcc ggcatcgtag tttcgcgcgc ggtgattcgc gacctgttcc cgccggccca
    19201 ggcgcagcag gtgatgagcc aggtgaccat cttcttcggt gtggcgccgg cgatcgcccc
    19261 catcgtcggc ggctggctgt cggtgcacct gggctggcac agcgtgttct ggtttctgac
    19321 cggggtgggc ctgtttctgt ggctggccaa ctggcggctg ctacccgagt cgctgccgcc
    19381 cgagggtcgc cagtccatcc atctggggca cctgatgcgc ggctattggg agctgatttc
    19441 cagcccgcgc ttcatgctgt tggcactggc cagcggcgtt cccttcaatg gcatgttttt
    19501 gtacgtgctg tcggctccgg cctttctggg cgacctgctg gggctggcgc cacagcagtt
    19561 cttctggttc tttctgtgca ccatctccgg catcatgggc ggcgcctgga tgagcgggcg
    19621 gctggcgggc cgcataccgc ccaagcacca gatccgccac ggcttcgtca tcatgctggc
    19681 catgggcctc ttgaacctgg gtgccaacct gttgtttccg gcccatgtcg ggtgggcgct
    19741 gtggcccatc ggcatcttct cgtttggctg ggcgctgatg gtgccggtgg tcacgctgct
    19801 ggtgctggat ctgtttccgg cacgccgcgg catggcgtcc tcgctgcagg cctttgtcgg
    19861 ctccacggcc aacgggctgg tcgcaggtgt ggtggcgccg ctggtgatgc actccacggt
    19921 gctactggcc acggcctcgc tgctgatgat gtgcgtgggc ctggtgtcct ggatctacct
    19981 gcaccatcgc tggcccgaga tcgggcgcac cgccacgcat tcctgaaaca gcttcaggcg
    20041 tggcgacggt cgagccccat ggccgcgtag tagcgagcct tctcggcgta catggcctgg
    20101 tcggcctggt gcgcggcggc ctcgatctga tcgcctgaca tggccacggc cacgcccatg
    20161 gacaggctca gggcctggcc cgggtagaac tggttgttca gctcctgcag gctgatgatg
    20221 cgctccatca gtgcctgcgc gccgcgctcg tcggtcatgg gcagcagtac gcagaactcg
    20281 tccccgccta tgcgcgccgt gcatgccgag gcatcgacgg ccttggccag cacctcgcct
    20341 gcgcgacgca gcatggcgtc gccagcggca tggccatgct cgtcgttgat ggccttcagg
    20401 ccattgagat cgatggcgat caccgccacc ggccagggcc ccttgcgcgt gagccggttg
    20461 agctcctcca cgtagtaggc gcggttgcgc aggccggtga gcacatcatg cttgcccagg
    20521 tattccagat aggcctcggc cttcttgcgc gccgagatgt ccaccatgga cagcagcacc
    20581 atgccccagt cgtggcggtg ctcctgcagc acggcaaact gcatgtgcag atgcaccggc
    20641 tcgccgatca ggctgtagtt gaccgcctcg cgctgctgga acagcttgcc ctcccacaga
    20701 tcaagcagct gctcgctgaa cgaatcatgc atctcgccac ggaacacctt gtccaggccg
    20761 gccagcaggg tctgcttgtc gggggcgcca aacatgctca gcgtgagctg gttcacatcc
    20821 agcacacgga tctcgcgcat gcagcgcgag acgaactctg gatgcacctt cagaaaggtc
    20881 ttcaggtcgc tgatgccctg ggcacgcacc tcatcgagca ggggcttgac ggcgctgaaa
    20941 tcctctaccc atagcgacac gggcgaatgc tcgaacaggc cgcgcgcgta tttctcgctg
    21001 cgctccaggc gcagccgtgc ctgcacgcgc tcggtcacgt cctccagcga caccagcacc
    21061 cggctccagc agccctcgta gcccggcagg atgcgcccgc ggatgcgcac gtccaggcgc
    21121 cgcccgtcga gcgcatagtt caccgtctgg ttggcatact cgggcacccc gtcccacagc
    21181 tgcgcgatct cggcaatggc gctctcgaac atgtcgccgc gaaacacctg atccagcgaa
    21241 gcctccagcg tcgcctggtc gggcgcggca aacagctcca gcgtgcgccg gttcacgcgc
    21301 agcagccgga tgctggcgcc ataggcgcgc acgcactcgg ggtgccgacg cagatgcggc
    21361 accacgtcca ccacgcctgc ggcgcgccac tgctcgaaca gccggcgcac ggcgctgtag
    21421 tcctccagcc agagcgagac aggggcgagc tcgaacatat gctcgaaatc ggttgacggg
    21481 acaggcagca tgcacaactc cagatatcca ggaattatcg gcagcggccg gccggtcttg
    21541 atgcaaaaaa gccagccgcg ttgccggggc tggcttgtct cacgctgcgg gcgggcgggc
    21601 cgtccgcctg atcaacgatc gttgcgggcc gggcgcttgc gcgcctcgtg cggcgtgaag
    21661 gacttgccgg tgccgccatg gggcttggca aagccgccct tgccaaagcc cggcttggca
    21721 aagtcgctct tgcggccaaa gtcaccgcgc ggcgcaccgc cgtcgccacg gccatggtgg
    21781 tggccatggg gggcatgggc atggcgcggg ccgtcattga aacggggggc gtcgccaaaa
    21841 cgagacgttt caccaaaacg agacgtttca ccaaaacggg acgtttcacc aaacccgccc
    21901 ttgcggccat agccgtcgcc gtcacggcga gcaccaaagc cgccacgatc gccgccaaag
    21961 ccgccgcgcg ggccgcgctc gccaaagccg ccgcgatcac caaaaccgcc acggtcgcca
    22021 tggccacggc cggcgccgcc aaagcgctgg tcgcgcccac ggccgccgcc ctcgcggcca
    22081 ccacggccgg caaagtcggg gcggccaccc tgggggaagc gctgtgtggg ctccaggcct
    22141 tccaccacct cggccttgaa ctgctggcgg ctgtagccct cgatgtcaaa gatcttgcgg
    22201 cggtcgcgga actcggcaaa ggtcacggcc aggccgtcgc ggccggcgcg gccggtgcgg
    22261 ccgatgcggt gcgtgtagtc ctcggccttc atcggcaaac cgaaattgaa gacatgggtg
    22321 atggtgggca cgtcgatgcc gcgtgcggcc acgtcggtgg ccaccaggat ctgcacctgg
    22381 ccattgcgca gcgccatcag gcggcggttg cgcaggccct ggctcagagc gccatgcagc
    22441 gccacggcgc tgaagccatc ctgctgcagg tcattcgcca ggccgtcgca ctccacctgg
    22501 gtgctggcaa acacgatggc ctggtcgatg gaagcgtcgc gcagccagtg gtccaggagc
    22561 ttcctcttgt gctgggcgtt gtcggcccag aacagcttct gctggatgtt ggaatgcttc
    22621 tcctgcggcg agtcgatctg gatcttcttc acgctggcgc cgccatcgtg catcacgcgc
    22681 atggccagct gctggatgcg cggcgcaaag gtggcgctga acatcatggt ctgcttgcgt
    22741 tgggccgtca gctcattgat ctcggccagg tcgtcggaga aacccagatc cagcatgcga
    22801 tcggcctcgt ccacaaccag gaactgcacc tggtccagct tgatctgctg tgagcgctgc
    22861 aggtccagca ggcggccggg cgtggccacc accaggttgg cgttctgcag cttggcgatc
    22921 tgcatctggt agggcatgcc gcccaccacg ttggcgatgc gcaggccctt gcaatgcttg
    22981 accaggtcga tggcgtcatg cgccacctgc tgcgccagct cgcgcgtggg gcacagcacc
    23041 agggcgccgg gcacggcggc cttgaagttg cgcgcctgca tggggttctt gcgcttgggg
    23101 cgcttggggg ccgcctcgcc acgtgccgcg gcctcaagag cggcgcgctc atactcggca
    23161 cgctgcgcgg cctcggcctc ggcctgctgg cgcagcagcg tgtgcagcac gggcagcagg
    23221 aaggccgcgg tcttgccgct gccggtctgg ctggagacca tcaggtcgat gaagcggccg
    23281 tcctcggcgc cggcgcccat ggccagggga atggccttgt cctgcacggc cgtgggctgg
    23341 gtgtagccca ggtcggccac ggcctgcacc agctcgggcg ccaggcccag ctcgacaaag
    23401 ccattgggca cggcggcttc agggactgcg ttgatggcgg aaataacgga atcggcaggc
    23461 gcgcactcgc cctgcaacag aacttgctca gtcatgattt gctcacacga agacggccgc
    23521 aggcgctacc tgggcaggct tcgctcgatg gttacaaaac atcaaccatc aaacgaaacc
    23581 aagggccgca gcggacgacc cgggtgggca ttggttgcaa ggcgccgcgt ggctgtcacg
    23641 ctggcgtgca gtgcccgatg tgtgaaggga gatgcacaaa aaaattgcac cgcgctcttg
    23701 gcggtgcaag cggcgaatta tggcatgacc cgggttttcc cgcaagcgat ggttttgcag
    23761 gtcaaagcta acatgcatca agtcgatgca cttgtgcacc ttgcaggagg acgccatgca
    23821 gctaccgctg aacatcgtcg cccacggcag cgaaagcctg caacagcaga tcacgcagca
    23881 gatccgctgc ctgattcatg acggccgcct ggcgcccggt acgcgcatgc ctgccacgcg
    23941 cgagctggcg agcgacctgg gcgtgtcgcg caataccgtg attggcgcct tccagcgcct
    24001 gattgccgag ggctgcctgg aatcgcgcga gcccgtgggc acctttgtcg cacagcgcat
    24061 catcgtcgat cgggtagcgc cgcccgtagt gacgggccag gccacccctc ctttcacgtc
    24121 agcggcgcgg caagacaccg gcatgcaccg gccgcaaccg cgcctgcgcc cgccgcaaca
    24181 ctggcagcca caccatgtcg cgcgcccgca tgcgggccag gtggggctgg acttttgggt
    24241 tggccgaccg gacgccaggc tgtttccgct gcgcagctgg ggcatgctgc ttgcacgggc
    24301 gctgcacagc cagccacacc agctgtgcga atacggcgac ccgcagggca tgctggcgct
    24361 gcgcgaggcc atcgccagcc atattggcgc cacgcgcggc attgccaccg acgccggccg
    24421 cgtgctcatc accaacggca tccaggaggg cctgagcctg ctggctcaac tgctgattgc
    24481 cccgggcgtg gaggtggccg tggaaaaccc ctgctacatg ggagccagcc gcgtgtttgc
    24541 ggcgcatggc ggggtgctgg cgggcgtgcc ggtggacgac cagggcatgc gcacggatgc
    24601 actgccgcgc aaggcggcgc tggcctatgt cacgccctcg caccagtatc cgcttggcgg
    24661 cacgctgccg ctggagcgac gcgaggccct gctggcctgg gcgcgcaact gcggtgccta
    24721 tgtgctggaa gacgactacg acagcgactt tttctacgac cgtgcgccgc tgctggctct
    24781 gaaaagcctg gatcaccatg accaggtggt gtacctgggc actttctcca agacccttgg
    24841 cgcgggcctg cgcattggct acatggtgct gccgcacagt ctgtgccagg cagcagtgaa
    24901 cgccaaggcc atgctgaaca acagccagtc ctggctggag caggcggcgc tggcagcctt
    24961 catggccgag ggtggctatg aacatcatgt gcgccgcctg cggcgcctgt acgcggatcg
    25021 gcgcgaccac ctgcgcacgg ccctggggca atggctgcca gactggcagg tgcagggcgc
    25081 gggcagcggc atgcacctgg ttgccagggt gccgccaaat gccgcaccga ttcatgagct
    25141 ggaacgcgcg gcccacgccc agggggtggg catctatggt gtcgagcgcg gcaatggtca
    25201 attgctgaac gtggcagcgg atcacccctg gcgacgcatc gtgctgctgg gctatgcctc
    25261 gctcgacgag ccacaaatca gcgatgcgct gttgaggctg aggcaggttt ttccccattc
    25321 ttgagctcag ccgagcggcc caccaccttg tcgagcacca gcctgaggtt gctgctgccc
    25381 agcgccacgc gctccaaacg gccctctatg tcgcccgcct gggccaccgg gctgcccagg
    25441 cgcgagatgc gggccacgat atccacctgg gaaaagctgg acagcgccct gccctgcccc
    25501 atggcactgc tgtcatcgag ctggaaggcc acgggccagc ccccggcgcg ggtgcgcatg
    25561 gcggcgaccg gcatgggcgg accgccgggc gcgcgggcaa agatgaacag ggtgtcggtg
    25621 ggggccaccc tggccgcaag ctcgctggcc accgtcaccg tgccgctgat gcccgccttg
    25681 ctggcgggcg cctggtccgc cacgcgcgcc atgacaggtg cagtgtcggg atggccggcc
    25741 ggcagggctg ccgggggcgg tggagcctcg cccctggcgc gcgccagcag cacgcgcacg
    25801 cgctcaaggt cttgggggtc ggccgccacc agctgctcca gccggctcca gtgctggagg
    25861 gcgccgtccc ggtcgccggc ctgcagggcc gcagccccgg ccagcgccag ggcattcagg
    25921 ttgtcggcct cgatgcgcag ggcacgcgcc agcagatcct ggggctggcc gctcaaatcg
    25981 cgctgctggg tcatggccac ggcattggcg tattcaacca gcaggttggc gtcgtcaggc
    26041 cgcagctcgc tggccttggc gtaggcccgc acggcctcgg cggcacgccc tgcggacagc
    26101 tgggaacgcg ccagcagcat ccagccatcg gcatcgccac cagtgctttc caggcgctgg
    26161 gccagcgcct gtaccgctgc gtccatttgc cgttcatgga ctggaggact ctgccctgcc
    26221 agcatgagtg cgccgggctg gcccaccacc aggtacagcg caaccgccat caaaggcaag
    26281 gcgatgccca agcccagcgc ggcaccccgg ttggcgccgc ggctgcgctc ttgctgctgc
    26341 tggcgcatgg catcggccgc cagtgcctgg cggcgcaggg catcgccgct gcgaccatgc
    26401 tccagcgcgt cgatgcggcc ggccgccaga tcctcatcga gctcgcgcat ggcatcggcc
    26461 aacacggtca ggttcgcgcc cgcgccggtg cccgcagcac gcgtgcgcag caggggcaag
    26521 accacaaagg ccagggccag tgccagcatg gctgccgcca gcagaacaaa cccggtcatg
    26581 tgtgccacct ccatctcatc gccgcgcctc tttcaatgcc acggtgcccc ggccgcgctc
    26641 tgcgcgcacc acggcgccag gtggcgccgg ttgcgcggca gccagcgcgc gtggccggta
    26701 gcggcgatcg agcacggccc aaaagccccc cagggccatg aacccacagc cccaccagat
    26761 ccaggcgatg aagggcttgt agtacacgcg caccacccag cgattgccgc ccagggactc
    26821 tcccagcgcc gcatacaggt cgcgcgccag cccgctgtgg atggcggcct cggtcatggg
    26881 cgatggattg cggccggcgg tatacaggcg ccgctcgggt tgcagcgtgg ccacgaccct
    26941 gtcgccccgc agcacctgca gtgtggccac gtcggcgagg tagttggggc cctgctggcg
    27001 ccgcacacca tcaaagcgga attcatagcc cgccagggtg gtggacaggc caggctccat
    27061 ggtcacgtcc ctttccagct catagccctt gaccagggtg acgccgacga tgaacaccgc
    27121 caggccgcaa tgcgccagca gcatgccgta gtagctgcgc ggctgtttgc gcacgcgcag
    27181 ccagagccca tcgcggccca tcgggcgcca ctggcggatc aggctcagga cagtggtggc
    27241 aaaaatccag aaagcgaaaa acaggccaag tcccaccagc gccgaccagc cgcccatgag
    27301 ccagggcgcc agcagggccg aggcaaggct caccgccagc gcccagcgca gcaggcgtgc
    27361 cagatccggc acctggtcgc cgccccagcg cgcgagtggc cccacgccca tgaggaacag
    27421 cgccggtgcc atcagcggca cgaggacgct gtcgaaatac ggcggcccca ccgacagctt
    27481 gcccagaccc agcgcatcga gcagcaaggg gtacagcgtg cccagcagca cggccgcgca
    27541 ggccacgagc agcagcaggt tgttcgccag cagcatggct tcgcgcgaca gcaggtcgaa
    27601 gcggctgccg ccgccgacca gcggcgcacg ccaggcaaac aggatcagtg agccgccgat
    27661 gacgatgatc agaaagccca ggatgtagat gcctcgcgat ggatcggtgg caaaggcatg
    27721 caccgaggtc agcacgcccg agcgcaccag gaaggtgccc agcagagaca aggcaaaggc
    27781 gatgatggcc agcagcgccg tccacatgcg aaagccgccg cgcttttcgg tcaccgccaa
    27841 tgagtgcagc agggccgtgc tgaccagcca gggcatgagc gaggcgttct ccaccggatc
    27901 ccagaaccac cagccacccc agcccagctc gtagtaggcc caggcgctgc ccagcgctat
    27961 gcccagcgtg agaaagcacc acgacaccgt ggcccagggc cgcgaccagc gcgcccaggc
    28021 ggcgtccagc cgtcccgcca gcagggcaga gatggcaaag gcaaacgcca ccaccgtgcc
    28081 cacgtaaccc atgtagagca gcggcgggtg aaagatcagg ccgggatcct gcagctgcgg
    28141 attcaggtcc tggccgtcgg gcgcggccgg cagcaggcgg ctgaaggggt tggaggtgaa
    28201 caggatgaag gccagaaagc ccacgctgat cagccccatc acgcccagga tgcgggcacg
    28261 cgcaggctcg tccaggtggc gcgagaagaa cgacaccgcc acgctccagc cgcccagaat
    28321 cagggcccac agcaggatgg agccctcatg cccgccccat acgccggcaa tacggtaagg
    28381 caggggcagg gccgagttgg aatgctgcac cacatacagc acggaaaaat cattgctcac
    28441 gaaggcatgg gtcaggcagg cgaaggccat cgccacgaac aggcattgcc cgcgcgccgc
    28501 agggcgggca aaggccatcc agtcgccatt gccacgcgcc gcgccgacga gcggcaacac
    28561 gccctgggcc agcgccagca ccaaggccag caccagggca aactggccca gctcggcgct
    28621 catgggcggg cctgcggcgc cagctcgtag accacgaagc cgcccacgcg gtacaccagc
    28681 gtggcaccgg ccacatcctg ctggtcgttg cccatgaagg gcagcagccg ggcggccagg
    28741 gtcttgcccg aggccgcatc ggtcagcgcc acggcgcgca ccgggctgtc cttgtcctgc
    28801 agcatggcgt aggccgggta gcggttgtga tccagccagt cgtggacgcg ccgcaccatg
    28861 ccatgcacgt cgctcaaggc gccgaggtcg gtaaaggcca cgcccaggcg gcgcgaatcc
    28921 atctggccga gcaggatggc atcgcgcaca tgcagcacca gcacggccgg cttgccgccg
    28981 gcgatggcct gcaactcggc catgccactg gcctcgtccg agagcagcgc attggcaaag
    29041 cgctggaact gcacacgctc ttctcctgcg ttgccggcct tccagaacgc acgctctatc
    29101 gactctatgt ccgcgctctg cgacctccag cgctgcggca caaagagggg caggcccaga
    29161 tgtttgccaa aacgcaccgg cgtgccgccg agcaactgca gctggcgcga ggtgtcccac
    29221 cagccaagca ccaggccgtc cttgggcatg tggcgctgca gcacctgtgc cagcgcactc
    29281 acatcctgtg ccgacgtacc cacggtgaga aaaggatcat caacccggga cttccacttc
    29341 agcagcacgg ggccggaaga tacttcggcc aactccagct cagccagagg ctgctggtct
    29401 gccgcaatga tgctggcacg acgcaccacc acctgccgct cggccaggct ggcccaagcg
    29461 tccagctcgc ccgcgggaac gacggccccg agctcgtagt ggtagcttgc cggactgctc
    29521 acatgcttcc aagcaaaaag ccctgcaacg cttatcagta aagcgccaac agctatcgaa
    29581 aacaaagcaa atctgcgctt ccaatcggct tgtcgatgat gcgccgggaa cgtggcctgg
    29641 tcatgggcat gtgcgaccag catcaccgtg tctcctgttc gggcagggtg gtcgcatcgc
    29701 ttgcctgccg ccctgggagc cgacggcggc ggcgcacggt ggaggcaccg ccgacgacca
    29761 gggtgccgcc gagcagcgac aaggccaggc tggaggtgag cgggtcgtca gtcacggcgg
    29821 ccaggacgga ctgggggcca tcgtccgggg agcggttcag gaacgaggtc ttggcgcggc
    29881 cgctctccag cttgcgcagg cgctgctcca tcctggcgtt ctcgcgcagc acggtgtctt
    29941 gctcgttgat gatggtctgg tccttgatca ggtcgctcca gccgtgcgag taggtccagc
    30001 caccggggtt cacatgctcc aggcctttca tgtagcgcgc cacatgctgc tcccacatct
    30061 cggcaaaggt gcgatcggcc acggtggtct ggttattggt ggcaaaaaac agtgacgaga
    30121 agcctccggg ttcgtccttc tccggggccg gcaagggcgt gcggttggtc ttctgcccga
    30181 ccagcagttt gtcgtcgtag agcttttgca tcaccttgcg cgactgctcg accaggccgg
    30241 tgccgagctt gatgccatcg tccatcatgg tcagataggt ctgggcaaag cgcggcgcat
    30301 ggcattggga acaggtcgcc gcccaggcgt ccttgcgctc cttgaaccag gggtgctcca
    30361 ggttgtcggc aatgctcttg aacggcagaa agccccagcg caccttgcgc accacgttgt
    30421 gcgagtactg gcccttgtac tcaaagtggc aggtctggca ggtgggcgcc gtggcgccgt
    30481 tcttgtggcc cagctggtcg gccagacgga tgttccagtc ccattggtta cccagcgtct
    30541 gataggccgt gccatgcttg gaatacatgt actgctcgaa ctcgttgtga tccacgccgt
    30601 tgtggcaggt ggcgcaggcc tcgggcttgc gtgactcgac ggtggaaaac tcgtggcgcg
    30661 tgtggcagtt gtcgcacttg gtctggttgg tatggcacat ggtgcagctg gcggccaccg
    30721 ggcccttgcc ctcgttcatc agcacggccc aggtggcggt ctccacgttc gcacggtagt
    30781 ccacggtatg tgacgggtgg ccctgatccc attgcttctg cggccacacc agggtgtcac
    30841 gctcggactc ggcctcggca aactggcgca catggcagga gccgcacacg gcccggtcgg
    30901 gcatgcgcag gtccttcacg tggttgccgc ccttggcgcc tatgcccatg tggcagtcgg
    30961 tgcactgcac ctccttcagc ggctcggtct tggccagcag cccctggccg cgcaggttgt
    31021 cctcaacctc ggcaatgatg tccttcttgt aggcacgcac gtcggatgcc ggcagctggc
    31081 ggatggcgtc gaggttggcg tgcacgctct tttcccagga cttgacccag ccatgggtga
    31141 cactggtgtg gcagctcacg cactgctcgc gcgtgacgtc gatgttcacg tctggtggct
    31201 tgtagaacag gctgggcgca aggtacatgt ccatgggaat aggctcccag tacctggcca
    31261 gcttgccctt gttcagctcg gccttgtagc gcttggacag ggcctcgaac aattccttgg
    31321 gcgaggcctc gcggctcagc tgcagggcct gcagctgctc gtcgggcacc gaatcccaga
    31381 tacgcgcctg cgccggcatg tgagacgcga gcagggccac tgctgcaatg agaagagaca
    31441 gccatcgtgt cagcatctgc aacctccagt ccgccataca gccaagctgc gctggatcct
    31501 gaagattgga cgcatgcggc accgcaaggg ttaccgccgt ctcaacccgc cattccatgt
    31561 ggatgaacgc agctttgcgc ctttgccaca tggcagaacc gcccgcgact tgtgatttac
    31621 gccaccgtac gcgtgactat gggcggcctg cgggtggttt tgaaagaacc acagcccagg
    31681 cggtggatgg aaccacggca gtttcgagcg ggcaccgcag cggcacgccg ccgcaggggg
    31741 ccaaagaaaa gggggctttg aagcccccct tgctgtggta ttggccgcag gccgttatcg
    31801 ccaggctctc aggttcagta gttggccagc ggcttggcct tgccgccctc gaacgtcatg
    31861 acgacgatgg gcgccttgat gcgattgccc ttgtcgtcgt acgcatactc gcccatcacg
    31921 cccttgtagg tctgctggtg catgtaagcg ccgaccttgg cgggatcgat ggagccgctc
    31981 ttttgcatgg cctcgccaat gttcatgacc tggtcgtagt agaacgggcc atagacatcg
    32041 gggttctggc cgaagcgctt cttgaacttc tcctggaagg ccttggcacc ggcgtcgctg
    32101 tccgtcagca tgccggcata ggcgcaatag accacgttgt tcacggcgtc ggcgcccagc
    32161 ttgggcatct cggggctgca cagcgtgtcg ccgcccagca gcttggcgtt gatgcccagc
    32221 tgcttcatct ggcgcgccat gggcgctgcc tgcggggcgt agccgccgta gaagatgacc
    32281 tcgggctgct tggccttcat gttggtcaga atggccatga aatcggtggc cttgtcggtc
    32341 gtgaactcgc ggccgaccac ggtcaggccg agcttctgcg cctccttggc gaactcatcg
    32401 gccagcccct ggccaaaggc cgtacggtcg tcgatcacgc cgaccttctt gaccttgagc
    32461 acgttggccg cataaccggc catgttggag ccggccttct catcgctggc cacgatgcga
    32521 aacaggttct tgtagccgcc ctgggtgatc ttggggttgg tgcccacggt ggagaccatg
    32581 gtgccgccgt tgtcatagac gcgcgatgcc ggaatggtca cgcccgagca gtacggcccc
    32641 atgacatagc gcacgcccga atccacaaac ttctgcgcca cggccacgcc ggccttggcg
    32701 tcgcactggt cgtcctcgga caccagctcg aacttcagcg tcttgccacc cacgttgatc
    32761 ttgcgcgcgt tcaggtcctc gatggccagg cgcacgccgt tgtcgttgtc gcggccggcg
    32821 aaggtgttag ggcccgacag ggggccgctg tggccgatct tgacgacctg ctcctgggcc
    32881 agggcgccag aggcgcaaac cagggcggca gcggccacga gggtggattt catgatgggg
    32941 gattgcatgg cgaacattcc ggttgagatt gtgcgaaaca tgcccgtcaa tgccaggcac
    33001 ttcgcactgg cctggacgac gcaaggtctg tgtgcagaca accggcgatt atctgacgaa
    33061 cccgctcaac tttacaaggc cagcaactgc tggcggtagt gctgcagttc gtcgatggac
    33121 tcatgcacat cggccagggc ggtgtggcgc tgcgccttct tgaagctggc gtaggcctcg
    33181 ggcttccagc gcttggccag ctccttgagc gtgctcacat ccacattgcg gtagtgaaag
    33241 aaggcctcca gcctgggcat gtacttgacc aggaaacgcc tgtcctggcc gatgctgttg
    33301 ccgcacatgg gcacgcagcc cttgggcaca tagagcgaca ggaagtcgat gagctgctgc
    33361 tcggcatcgg cctcgctgac ggtggaggcg cgcaccttgt cgatcaagcc gctcttgcca
    33421 tgggtgccct tgttccaggc atccatggcg tcgagcacgg cgtcgctctg gtggatggcg
    33481 aacaccgggc cttcgacgcg cggctccagg ctggggccgg tgacgaccac ggcgatctcc
    33541 agcaggtatt ccttttcggg gtcgagcccg ctcatttcac agtcgagcca gacaaggttc
    33601 tggtcggatt tggcaagggt gggggcagtg ggattgacag tttcagacat ggcgcaaatt
    33661 gtcgccgtaa acttgcgcgg ccatgccgcc ctcctctacc gcccccttcc tctcgccctc
    33721 gaccttgacg accctggtgt ttgccgccct gctgctggcc gggctggcgt tgcgcctgtg
    33781 gctggccacg cgccagatcc gccatgtggc gcagcaccgc ggcgccgtgc cggcggcctt
    33841 tgccgagcgc atcaccctgg ccgcgcacca gaaggcggcc gactacacca tcgccaaggc
    33901 gcgctttggc ctgctctcta tggccctgtc caccgccgtg gtgctgggct ggacgctgct
    33961 gggcgggctg gacgccctga accagcagct gctggcctgg ctgggcggcg gcatgccgca
    34021 gcagctggcg ctgctggccg gctttgccct catcggtggc gccctggatc tgccggccag
    34081 cctgtaccag acctttcgca tcgagcagcg ctttggtttc aaccagatga cgccgcggct
    34141 gtggctgacc gatctggtca agtccacgct gatcggggcc gtgatcggcc tgccgatcgc
    34201 cgcgctgatc ctgtggctca tgggcgccgc cggcccgctg tggtggctct gggcctgggg
    34261 cgtgtggatg ggcttcaacc tgctgctgat ggtggtgttc ccggtgttca tcgcgccgct
    34321 gttcaacaaa ttccagccgc ttgccgacga ggccctgaag gcgcgtgtga cggcgctgat
    34381 gcagcgctgc ggctttgccg ccaagggcct gttcgtgatg gacggcagcc ggcgcagtgc
    34441 acacgccaac gcctacttca cgggtttcgg cgcggccaag cgcgtggtgt tcttcgacac
    34501 gctgctcagg cagctctcgc ccgccgaggt cgaggccgtg ctggcgcatg agctgggcca
    34561 cttcaagcac aaacatgtca cgcgccgcat gctgggcatg tttgccgtca gcctggccgg
    34621 ctttgcactg ctcggctggc tgtccacgcg cggctggttc tacaccggcc tgggcgtgca
    34681 acccaacctg atgatcgagg gcctgcccgg ctcggcgccc aacgacgcgc tggcgctgct
    34741 gctgttcctg ctggccgtgc cggtgctgac gctgttcgcc cagccgctga tggcgcagat
    34801 ctcgcgccac caggagtttc aggccgatgc ctatgcagtc agccaggcca gcggcaccga
    34861 gctggcgtcg gccctgctca agctctacca ggacaatgcc tccacgctca cgccagaccc
    34921 ggtgtacgtg aagttctact actcccaccc ccccgccacg gagcgcctgg cgcgcctgcc
    34981 cgtggccgtc cagtcatgac ctcgatgttt ccaaaaaaag actggtctac gcagaccagg
    35041 cgcgcgctgt cagctactga aatagtagtc aaactgaccg aactgccagg ctggcagctt
    35101 tccggcgacg gcgccgatgt ggccatagag aagacctacc gcttcgccaa ctaccacgag
    35161 accatggcct tcgtgaacgc cgtggccttc atcgcccatg cgcaggacca ccatccggat
    35221 ctgtcggtgc attacgcccg ctgcgtggtg cgcctgaata cgcatgacgt gggcggcatc
    35281 tcggccaccg acatcgactg cgccacgcgc atagacgccc tgctggccgc gccatgacgc
    35341 gcagcgaagg cctggtggtc gccagccatg gccgccactg cgtggtggag agcgccgacg
    35401 gcgtgcgccg catctgccac ccgcgcggca agaagagcca ggccgtcgtg ggcgaccgcg
    35461 tgctgtggca ggccgcggcc cccggccagg cgcagggcca tggcgacgag ggcacgatag
    35521 aaaaggtgct ggagcggcgc aacctgttct accgccagga cgaggtgcgc accaaatcct
    35581 ttgccgccaa cctcgatcag gtgctgatcc tgatcgccgc cgagcccgtg ttttccgaaa
    35641 gccagctcgt gcgcgccctg atcgcggccg aggctgccgg catcacaccg ctgatagcac
    35701 tcaacaaaag cgacctggtg gagccttttg cacaggcctg gcagcgcctg tggccctacc
    35761 gccatatggg tggcgagcag gacacgcgcc accactaccg cgtgctgccg ctgtcgctga
    35821 cgcaatcggg cgcggtcgat cgcgatgcac tgctgccgct gctggcgggc aagaccaccc
    35881 tgatcctggg tccttcgggg tcaggcaaga gcaccctggt gaacctgctg gtaccagggg
    35941 ctgcggcgca gacgggcgag atatcgcagg cactgaactc cggccggcac accaccacca
    36001 ccaccagcct gtactggata gacggcgcgc gcaccacggc gctcatcgac tcgccgggct
    36061 ttcaggagtt cggtctgcgt cacctggcgc ccaccgatct ggcgcgctgc atgcccgaca
    36121 ttgccgcgca tgccggcggg tgccgcttct acaactgcag ccacctgcac gagcccggct
    36181 gcgccgtgat ggcccaggtg agcgagagcc aggacgcgca ggccatcagc ctgcagcgct
    36241 accgcatcta tagcgaattg ttcgccgaac tgagccagag cgcctattga tggatattgg
    36301 acaaatacgg ttgtagcgcc tgccaataaa gcgtaaatag cttttaattt gatagcatca
    36361 accgagcagg cgcgccaggg tcagcagcgc cagcagcagc atccacacca cgaccgagcg
    36421 ccacaccagg cccaccacgc tgcgcaggtg gcccacctcc ggctcgcggc ccggcgtgct
    36481 gctgctgtcc gccatgtcgg ccccggcctc aaaatcggcg gcaagctcgg cgccggcgcg
    36541 gctgcgcagc gcctctcccc ctaagcgcac gttgatggcg ccggccgtgg cggccagcac
    36601 cacgccatcg ttgtcgttgg gaaagcgctg ggcatgaaat cgccagccgt cgatggcctc
    36661 ctcgaaactg cccaccacgg caaagctcat cgccgtcagg cgcgccggca gccagtcgat
    36721 aaccatccag gcctgctccg ccgcgcgttg cagtgactca ctgggcggat gggccgtgac
    36781 cacgcgcgac cctcgccccc agtagcgcgc cacaaattcg gccagccggt acaacaccgc
    36841 accggtcggc cccaggccca gcgccgcgaa caccgagtac cacgccagca caccaaacac
    36901 atggcggtgc gccgcaatca ccgaatactc gatcacatgg cgcacgatct cgctgcgcgg
    36961 cagctcgctg gcatccacct gcttccagcg tgcgagcagc gcgcgtgcct cggcggcgtc
    37021 gccatcctcc agagcatcac gtatggccgt gaagtgatga ctgaactggc gaaaacccag
    37081 cgtgaggtag agcacggcca cgctccacag cagcgccagc ggccagccca gccaatgcag
    37141 cagcgcccaa tgcacggcca gcacaaacgc cggcggcacg agtacggcca ggctccaggc
    37201 cacccagcca tgccgggact gtccggcatc aaaactgcgg cataccgaca gggcccaggc
    37261 gcgcaggccg gcgtggatgg ggttgctgcg ggccagcggc cgcgcctgct cgatcagcaa
    37321 ggcgaacagg atggcaaaga aactcatggc ggcaatgata gcggcgccgc ccggcaggcc
    37381 gccgcgcctg cagtcacgcc agcatgaagc ggtagaagtt gcgcagcatg gccgccgtgg
    37441 cgccccagat gtagcgctgg cgcgcaccgt cctggtaggg catggcaaac cactcacggc
    37501 gcaggccctg ccactccagc gcatggcgtt cgtggtgcgc cggattgagc aggaagtcga
    37561 gcggcacctc gaatacgtcg gccacctcgt ccgggttggg cgccagcaca aaatccggcc
    37621 gtaccagggc cacgacgggc gtgacgatga aggagctgcc agtcacatag atcggcagct
    37681 ggcccaacac ctccacgctg cccgcctcca gccccacttc ctcatgcgtc tcgcgcaggg
    37741 ctgtggcggc ggcatccaga tcctcggggt cgctgcgccc gccaggaaag gccacctgcc
    37801 ccttgtgcgt ggacaggtgg gccgtgcgtt cggtgagcag caccgtgggc cgctcacgct
    37861 gcacgatggg caccaacacc gccgcctcgg ccggtgggcg ctgcacgaac ttcttctcgc
    37921 gcacgatctc cggcgtccac agtggcggcg tggcaaagcg ccggcgcaat gcatcggggg
    37981 tctgcgccgc cgctgggacc gccggcaaat ggccgtccac acccaccagc ggcacggtac
    38041 gtgggtcaaa gtcgggcagc gacgacagcg aggaaatcac cgtcgccgca tcagagccaa
    38101 tggggcgaaa tgagctcaca aaacagcacc aggaatgaaa aaaccgctac aggcctgcgc
    38161 actgtagcgg ttttgcagct tgaggcttgc gccgcgtgaa ttacgcggcg gttgcagcca
    38221 ccttgacgcg ggagggcagc ttttccttga tgcgtgccga cttgccgctg cgctggcgca
    38281 ggtagtacag cttggcgcgg cgcacatcgc cacggcgctt gacttcgatg ccggcgatca
    38341 gcgggctgta ggtctggaac gtgcgctcca cgccttcgcc gctggagatc ttgcgcacgg
    38401 tgaagccgct gttcaggcca cggttgcgct tggcgatcac cacgccttcg taggcctgca
    38461 cgcgcttgcg gctgccttcg accacgttca cgctgacgat gacggtgtcg ccaggagcga
    38521 atgcgggaat ggtcttgttc aagcgggcga tttcttcctg ctcgaggatc tggatcagat
    38581 tcatggtttg gtttccaacg atcatggacg cgccaggatt ggctgcggcg cccctattgg
    38641 cgccttgtgg ctattcggct gatcatgctg cgcatgtgcc catagcggcc ggccagagga
    38701 tcgagaacct gcgattatag tgggttttgc gctagcactg cctcatctgc gcgcgtgagc
    38761 aacccacgct cgcgcgccgc ctggagcagg tcgggtcgat ggcgcgccgt ggtcacgagg
    38821 cgctgatcgc gacgccagcg ctcgatctgc gcatggtggc ccgacagcag cggagccggc
    38881 acgccgcggc cctgccattg ctcgggccgg gtgtagtgag ggcagtccag caggccatcc
    38941 agggccgggt tgaagctatc gagctggtga ctgccctcgt cgtgcagcac acccggctgc
    39001 aggcgcgcca cggcatccag cagggccatg gccgcgatct cgccgcccga gagcacaaag
    39061 tcgcccaggc tgatctgcat gtccacatgg gcgtcgatga agcgctggtc cacaccctca
    39121 tagcggccgc agagcagcac cgcacccgcc cccgccgacc aatcctgcac cgccgcgtga
    39181 tccagcctgc ggcctatggg cgagaacagc accagcggcg ccgcctgcgc ttctgcacga
    39241 tcggcgcgga tggcggccag acagcgcgcc agcggttcgg ccatcatcac catgccgggg
    39301 ccgccgccaa acgggcggtc atcgacgcgg cggtagttac cctcggcatg gtcgcgcagg
    39361 ctccacagcc gcacctgcac ctggcccgtg gcgtaggcac gccgcgtcac gccgctggcc
    39421 agaaagggct cgaacagctc ggggaacagg gtgatgacgt caaagcgcat gggggcttca
    39481 gtagtcaggt tgccagtcca ccctgatgca gcgcccggcc agatccacct tgtccacaaa
    39541 ggccgaaacg aaggggatca tgcgctcctg cgccttgccc tcatgctcgt agcccagcac
    39601 cagcgtggtc tgcggtccgg tgggcagcag atcacgcacg gtgcccagcg gcacgccctc
    39661 gcggttcacc acggccaggc cgatcaggtc gacccagtag tactcgtcat cgccgaccgc
    39721 aggaaaactc gcacgtggca caaagatgcg cgcgccgcgc agcgcctcgg cgccgtcgcg
    39781 atcgtccacg ccctgcgccc aggccacgat ggtgtcggaa tgcgtgcgtg cctggcgtat
    39841 cgggagcaac acggtgccgg caaagaagct cttggcgccc cgttcggcgg gctgcaaaaa
    39901 ccaggccctg gaggccagca aggcctcggg gtcgctgctg tagggcaaga ccttgaacca
    39961 gcccttcacg ccccaggcat cggtgatgcg gccgacctcc accgcgtcag ggggcagttc
    40021 actggcttcc agaatggggg gctgaggcat ggcggcggca gggcgaaaag aatcgtgcat
    40081 gaaaaaaggc ggggcccgtc agctgtacca acggggcccg cccttttcgg gactgtgaac
    40141 cgatcaggcg acctgcttgg cggcttgctt gatcaggcga tccacggtgg gggaggcttg
    40201 cgcgcccaca cccttccagt acgtcaggcg atcctgggcg atgcgcaggg tctcttcgcc
    40261 gcccttggcg gaagggttgt agaagcccag acgctcgatg aagcggccgt cacggcgcac
    40321 gcgcttgtcg gcaacgacga tgttgaaaaa cggacggccc ttggagccgc cgcgggagag
    40381 tcgaatcacg accataatga atccttcggg tggacgcagc ggttgctgca tgagtttttg
    40441 cggcgtttga gacacgcgac ttggccaccc ggccagcgac acgcagcaaa gccggcaatt
    40501 atagccccat ccacgcaaat gcccaccata cgcgccagca ttgacggcga catcgccgcc
    40561 atcaccgcca tctacgccca ccatgtgctg cacggcacgg gcaccttcga ggtcgacccg
    40621 cccagcagcc aggacatggc ggcgcgccgc gccgaggtgc tggccagggg cctgccccat
    40681 ctggtggccg aggaaggagg tcaggtactg ggctttgcct actgcaactg gttcaagccg
    40741 cgccccgcct atcgctattc ggccgaggac tcggtctacg tggccgatgc agcccggggg
    40801 ctcggcgtgg gccggttgct cctcgacgcc ctggccgagc aggcgcaggc cgcagggatg
    40861 cgcaagctgc tggccgtgat cggcgattcg gccaacgccg gctccatcgc cctgcaccgc
    40921 gctgccggat tcaccgaggt cggcgtgatg cgctccgtgg gctggaagtt tggcgcctgg
    40981 cgcgacatcg tgctcatgga aaagcccctg ggcgcaggcg acagcagcgc tcccgaataa
    41041 cgccacaacc atcaccccac ccatgaagaa caagaccctc gccacctggc tcgccttcct
    41101 cggggggccg ctggggctgc accgcttcta cctgtttggc tttggcgaca tgctcggctg
    41161 gctgctgccg gtgccgacgg cgctgggggt gtacggccta cagcgcgtgc agcaatgggg
    41221 tcaggacgat cagctgagct ggctgttgat cccgctgctg ggtttcacga tcgccggctg
    41281 cgcgctgtgc gccatcgtct acgggttgat ggccccggaa aaatggaatg cccgcttcaa
    41341 ccagaccgcc gcggccgacg ccgcgcctgg gcgcaccaac tggttcacgg tgtttgccgt
    41401 ggcgctatcg ctgctggtgg gcactgccgt gctgatggcc agcattgcct tcagcctgca
    41461 gcgctactac gaataccaga tcgaggaagc acgcaagatt tctcagtgaa aatggccgat
    41521 agcgcccatc catcaagcgc caatggctat tattttaata gtgatcaata cagctgcagg
    41581 ctgatccagt aggcaattgc ggcgacgaag gcgctggccg ggatggtcag aatccaggcc
    41641 cagacgatgt tgcccgccac accccagcgc acggcgctgg cgcgctgggt ggagcccacg
    41701 ccgacgatgg cgccggtgat ggtgtgggtg gtggacacgg gcacgcccag ggcggtggca
    41761 aagaacagcg tcagcgcccc gcccgactcg gcacaaaagc cgcccacggg cttgagcttg
    41821 gtgatcttct gccccatggt tttgacgatg cgccagccgc caaacatggt gcccaggccg
    41881 atggccgcgt agcacgagac gatgacccag gtgggcggcg cgctgtcagc agccgaggca
    41941 tagccggtgg cgatgagcag cagccagatg atgccaatgg ttttctgcgc gtcgttgcca
    42001 ccatggccca ggctgtaggc gccggccgac accagctgca ggcggcggaa ccatttgtcc
    42061 accttgatcg gtcgcgtgcg cctgaatacc caggccacca gcaccatcat cgccgagccc
    42121 agcaccatgc ccagcagcgg ggagatgaag atgaaggcca cggtcttgat gatgccactg
    42181 gccatcagcg cacccgagcc cgacttggcc accaccgcgc cgacgatgcc gccaatcagc
    42241 gcatgcgagg agctgctggg gatgccgtaa taccaggtga tgacgttcca ggtgatggcg
    42301 cccaccaggg ccccgaagat cacatgggtg tccaccacgc ccggatcggc aatgcccttg
    42361 ccgatggtgg cggccacgct gaggtggaac acgaagatcg ccacaaagtt gaaaaaggcc
    42421 gcaaagacaa cagcctgcgt gggtttgagc acacccgtgg aaaccacggt ggcaatcgag
    42481 ttggccgcgt catgaaaccc gttcatgaag tcgaacagaa tggcaagcgc caccagcacg
    42541 accacgaccc atagggcggt ttgtaccgta tccatggtgt agaaaagctt atgcctgggc
    42601 tcaggagttt tcgaggatca cgccctcgat gaggttggcc acgtcctcgc acttgtcggt
    42661 gatggtttcg agcagctcgt agatggcctt gagcttgatc agctcgcgca catcggtttc
    42721 ctcgcggaac agcttgctca tggcgctgcg cagcacgcgg tcggcgtcac cctccaggcg
    42781 atcgatctcc tcgcaggtct tgagcgcggc ctcggccacg gccggatcgg caatgcgcga
    42841 cagcatcttc acggcatcgc gcacgcgctc gcagcacttg acgctcaagt ccgtcaggcg
    42901 tgaaatttct tcggtcatgt ggcggatgtc atacagcgcc atggtctcgg ccgagtcctg
    42961 gatcaggtcg gccacatcgt ccatggtgtt gatcagtgag tgaatctgct cgcgatcgat
    43021 gggcgtgatg aacgtctggt gcagcgtgcg attgacctcg tgggtcacgc ggtcggcagc
    43081 gcgttcggcg ttgtccacgt cctggttgta ctggtcacgc agatgggtgt cactgtaatt
    43141 ggccaccagc tgcgaaaaag cgcgcgccgc ttcgacaata cggtcggcat gctggttgaa
    43201 catctcgaaa aaattgccct cgcggggcaa cagctttcca aacaacatgt cgactcctga
    43261 tcgaaccttc gtgcgtagta cgacgcagag ttgaccatcg tgtgacaaaa ctgcgaaagg
    43321 tgcgagttta accgccggct cgcaggctcc cggaatggca gggcaaaccc tgattgctgt
    43381 aggatttttc tcaccccgtg gcatcgccca ggcccacaag gcgcacccat tgccggccgt
    43441 agattgaagt acaaaagcca ctcaccaccc aggaggggcc atgccatcca cgcttcgtca
    43501 actgctctgc gccaccctgc tcacgggcct tgccgctgcc ggcctggcgc aagcgcccat
    43561 gccccgggct gcgggaatgt atggtggcta tgtgggactg ggcgcgggtg cctccaggta
    43621 tgacctgcgc agcggcagcg gcggtttcga cttcgacgat ggcggcagcg ccatcaagct
    43681 gtatgccggc gcgtttttcc accccaacct ggggctggag ctgggctacc tggatctggg
    43741 caaggcgcgc cgcatcggcg gcaacagcag cgcccgcggc ctgaacttca gcctggtggg
    43801 acgcctgccc ttgggcacgc agctggatct gcagggcaag ctgggcacct tctacggccg
    43861 caccgacacc cggggggtgg gcggctatgg cgtgcaactg ggcaagggaa gcggctttgg
    43921 cctgtcttac ggcgcagggc tgcgttgggc cttcacgccg caatggtctg ccgtgctgca
    43981 gtgggagcgc cagcgcctgc actttgccga ccgcctgtcg gacgtggatg cggccacgct
    44041 gggcctgcag tacaactact gacacgcccc aaaggacggg ccgttcaggc gctgcgaaag
    44101 atgaaataca ccgcgccgac caggcacaaa ccagcccaga gatagtccca tttgagcggc
    44161 tcgttcagat agaacaccgc gaaagggacg aacacggtca gcgtgatgac ctcctgcatg
    44221 atcttgagct ggcccacgct gaactgtgca tggccgatgc ggttggccgg cacctgcagc
    44281 aggtattcga acagcgcgat gccccaactc accagggccg ccacgtacca cggcgccgtg
    44341 gccagattct tcaggtgccc ataccaggca agggtcatga agacattgct tgccaccagc
    44401 agcaagatgg tctgcagcgg gatgggcagg gattgcagga catgcatggc gcagtcaaaa
    44461 tcactagaaa aatagcgccc tagcgcatat gcaacaagcg ccaatagcta ttaaaacaaa
    44521 agcaccacca gggcggtgcc ttggacttcg cgcggcttgc ctcactcgcc cagataagcc
    44581 gcgcgcacac gcggatccga cagcagatcc tgccccgcgc cggtcatggt gatcaggccg
    44641 gactccatca cgtagccgcg atcggcaatg gccagcgccc ggctggcgtt ctgctcgacc
    44701 agcacgatgg tcacgcccag ggcgtacacg tcgcgcacca cctcgaagat cttgtccacc
    44761 atgatgggcg acaggcccat ggagggctcg tccagcagca gcaccttggg ctggctcatc
    44821 agcgcgcggc ccatggccag catctgctgc tcgccgccgc tcatggtgcc ggccagctgg
    44881 tccttgcgct cgcgcagacg cgggaagatg ttgaacatct tttccacgtc cgcctcgatg
    44941 ccggccttgt cattgcgcgt ataggcgccc atgagcaggt tctcggtaat ggtcatgcgc
    45001 gcgaacacgc cgcggccctc gggcaccatc accaggccat cacgcaccag atcccaggcg
    45061 ccacggccct tgatgcttcg gcccaggtat tcgatgtcgc catcgttcat gcccaggccg
    45121 ccggtgatgg ccttcatgct ggtggtcttg ccggcgccgt tggagccgat cagcgtgacc
    45181 agctcgccct cgcgcacctc catgtccacg cccttgacgg cctggatgcc gccataggcc
    45241 accttcaggc ccgtgacctt gagcagaact tgcttgttgt tggtatcggc catatcaatg
    45301 cccccccgtt cccaggtagg cctcaatcac tttctcgttc ttctgcacct cggcaggcgt
    45361 gccttcggcg atctgcttgc catagtccag caccgtgacg cggtcgcaca ggcccatgat
    45421 gagcttgacg tcgtgttcga tgatgagaat ggttcggttg tcctggcgga tgcgctcgat
    45481 gagttcgcgc aactgcacct tctcggtggc gttcatgccg gccgcgggct cgtccagcgc
    45541 gatcagctgc ggatcggtgg ccagggcgcg cgcgatctcc aggcgccgct gatcgccgta
    45601 ggacagcgta cgcgccttga agtcggcgta cttgcccacg cccacgtagt ccagcagctc
    45661 ctgtgcgcgc ttggcgatcg cggcctcttc ctgcttgaag ccctgggtgc ggaacacggc
    45721 gccaaacagg cccgagtggg tgcggatatg ccgccccacc atcacgttct ccagcgccgt
    45781 catttcggca aacaggcgga tgttctgaaa ggtgcgcgca atgccggcct gggccacctt
    45841 gtgcaccgcc gtgggttcat agggccggcc cgcgagctcg aaggtacccg cgtcgggcgt
    45901 gtacaggccg gtgatgacgt tgaagaacgt cgtcttgccg gcgccattgg ggccgatcag
    45961 gccatagacc tgaccgcgct gtatggtgat gcccacgccc gacagcgcct gcaggccgcc
    46021 aaagcgtttg gaaatgcccg cgaccttgag aaccacgtct ttcgatgtat ctgtcatgtt
    46081 cttgctctat gcgtggtgtc aggacttgct cgccaggctc ttgccatgct cgggcgaagg
    46141 ccacaggcca cgcgggcgca gcagcatgat gatgatcatc gccagggcaa tcaacagctg
    46201 gcgcaggatg gacgcgtcca ggcgcccatc ggtcatggcc tgcagcgggc cggcgacata
    46261 gcgcagcacc tcgggcaggg ccgacagcag cacggcaccc aggatcacgc ccgggatatg
    46321 gcccaggccg cccagcacca ccatggcgac gatcatcacc gactccatca ggctgaagga
    46381 ctcgggcgag acaaaaccct ggaaggccgc gaacatggcg cccgagacgc cgccaaagga
    46441 tgcccccatg ccgaaggcca gcagcttcat gttgcgcgtg ttgatgccca tggccttggc
    46501 ggcgatctca tcttcgcgaa tggccatcca ggcgcggcct atgcgcgagt cctgcaggcg
    46561 atagcagatg acgatggtga tcaacaccag ggccaggaac aggtagtagt acatcgtcac
    46621 cgagttgatg tcgaagccga acagctcctg ccgctttgcc agatccaggc cgaagatctt
    46681 caccgagtcg atctgcccca ggcccttggg accattggtg aggttgaccg ggtgatccag
    46741 gttgttcagg aagatgcgga tgatttcacc gaagcccagc gtcacgatgg ccaggtagtc
    46801 gccgcgcagc ttgagcaccg gtatgcccag cagcaccccc gtgcaggccg ccagcaccag
    46861 ggcgagcgga atcaccaccc acagagacag gtgcatgcca tctgggaaat gcgcggcaaa
    46921 ccaggcaaag ttctccgtca ggtgcggcga ggccagcagg ccgaacatgt aggcgcccac
    46981 ggcgtagaag gccacgtagc ccagatccag caggccggca tagccgacca cgatgttcag
    47041 gcccagggcc agcatcacat acagcagtgc cagatcggca atgcgcaccc aggcgttgcc
    47101 aaagtactgc aacaccagcg gcagcaccag cagcgcgata ccacccagaa tccattggag
    47161 cttgttgttt ttcatgttgt ggctcctcgt taggcgcggt cggccacacg ttcacccagc
    47221 aggcccgagg ggcgcagcgt caggatgatg atgagcacga tgaaggcgaa gatgtcggtg
    47281 tagtggctgc ccagcacgcc accggtcagg gtgccgatgt agcccgagcc aatggcctcg
    47341 atcaggccca gcaacacacc gcccaccacg gcaccggtca ggttgccaat gccgccaaac
    47401 accgccgccg tgaaggcctt gagccccggc aggaaaccca tggtgtgctg ggccgtgccg
    47461 tagttggccg cccacatgat gccggcgatc gccgccagca ccgcgccgat gatgaaggtg
    47521 gccgagatca ccatgtcggg cttgacgccc atgagtgccg ccacacgcgg gttctcggca
    47581 gtggcgcgca tggcgcggcc caggcgggtg tagctcacca gatacaccat ggccgccagg
    47641 gcgatcaccg tgaccgccag gatcagcacc tgggtggtgg tgatcacggc gccgcccacc
    47701 tcgaacggcg cggccggcaa caaggtggga tagggcttgt aattgggctt ccagatgatc
    47761 atggccagcg tctgcagcag gatggacatg ccgatggctg tgatcagcgg tgccaggcgc
    47821 gggctgttgc gcaagcgctt gtaggcgacc ttctcgatcg tgtagttcaa cgccgccgcc
    47881 actacgcagg cgatcaccgt cgccagcagc agcacgagcc agcccggcgc gccaggcatg
    47941 gcgtcctgca tcaggccgat gcagctccag ctggtcagcg ccccgatcat gagcacctcc
    48001 ccatgggcga aattgatgag ctggatgatg ccgtacacca tggtatagcc caaggctatc
    48061 aaggcgtaca tgctgccgag aaccagacca ttgatgatct gttgcagcaa gatgtccata
    48121 agaaaaagtc ctttgtattg acgttcccag cagcaggcac ccatgcacgg tgcagccttg
    48181 tgggcccgtc tgccatttac caaaaaaccc gcgagccaaa ggtctgcgcg ggttggaagc
    48241 gggattttgt cacggctttg gcgccgccag cgggcagcct ggggcggtgt tttccctggc
    48301 tttcggtgca ggcgtttact tctgcgccgg caattttgca gcgctgttgc gtgcgcgcaa
    48361 ctcgtcaagc ttctggccta tcttgatctc caaaccgcgc ggcacggggg cgtaaaagcc
    48421 cgtcggctcc atgccatctg gcagatagct ttcgccggcg gcaaagccgc cctcctcgtc
    48481 atgtgcgtag cgatagccct tgccatagtc cagctgcttc atcagctgcg tgggcgcatt
    48541 gcgcaggtgc atgggcacgg ggcgggtgcc gtcctgcttc acaaaggcgc gcgccttgtt
    48601 gtaggccatg tagccggcgt tgctcttggg cgccaccgcc agatagagca cggcctgggc
    48661 cagggccagc tcaccctccg gcgagcccag gcgctcatag gtctgcgcgg cctcgttggc
    48721 gatctgcatg gcacgcgggt cggccaggcc gacatcctcc caggccatcc gcacgatgcg
    48781 ccgggccatg tagcgcgggt cggcgccgcc atcgagcatg cgcaccagcc agtacagcgc
    48841 ggcgtcgggg tcggagccgc gcaccgattt gtgcaacgca ctgatggtgt cgtagaactg
    48901 ctcaccaccc ttgtcgtagc ggcgcatgcg ctcaccaagc acgcgcacga cccagtcatc
    48961 ctcgatcagc gccaggcccg cttggctggc cgtgatggcc agcgtttcca gcgtattgag
    49021 caggcgccgc gcatcgccat cggcgtacgc aatcaggcgc tcagtcgctt cattttcaat
    49081 agctggtagc gcttgtaaat tctgggcttt ggcgattatt tgcttcaagt cttcgctgcc
    49141 caaaggctgc agcacataga ccacggcgcg cgacaacagc gccgagttga cctcgaacga
    49201 ggggttctcc gtcgtcgccc ccacgaaggt gaacagccca ctttccacat gcggcaagaa
    49261 agcatcctgc tggctcttgt tgaaacggtg cacctcgtcc acaaagacga tggtgcgctg
    49321 ccccatcaag ccgtcgcgtg cgctttcggc cagctgcacc gcatcgcgta tgtccttgac
    49381 cccgcccagc acggcgctga tgctgatgaa ctgggcgtcg aacgcctgcg ccatcaggcg
    49441 cgcaatggtg gtcttgccca cacccggtgg cccccacagg atgcagctgt gcggccgccc
    49501 cgactcgaac gccaggcgca gcggcatgcc cgggcccagc acatgctgct ggccaacgac
    49561 ctcaccgagg gtgcgcgggc gcaggcgttc ggccaggggt tgatgggcag cagcagcgtt
    49621 catgacaggc aggggcaggc aatccggcga tggtagcggg caaagccacc ggagatcgac
    49681 aatggccggt cacgcaagct gccatgcccc gccacgaatc actccacgcc ctgttgcacc
    49741 gcgtgcgcgc ctgcacgatc tgtgcggact acctgcccct tggcccccgg ccagtgcttc
    49801 aggccggcag cggtgcgcgc atactgattg caggccaggc gccggggcgc aaggtgcatg
    49861 cctcgggcat cccgtttgac gacgccagcg gccagcggct gcgcgactgg ctgggcctga
    49921 cgcccgagca gttctacgat ccaacgcagg tggccatcct gcccatgggt ttttgttatc
    49981 ccggcaaggg gatctcgggc gatgcgcctc cccgcccgga atgtgctccg gcgtggcgcg
    50041 cacagttgct ggcgcattta ccgcgtctgg ccttcaccgt ggtgctgggt cagtacgcgc
    50101 tcgcctggca tatgcccgaa gagcgctcgg gcgtgaccca ggcggtacaa aactggcagc
    50161 gccactggcc cgcggccgtc gtcctgcccc atcccagccc gcgcaacaac ggctggctca
    50221 agcgcaaccc atggttcgag acgcagttgc tgcccgaggt gcgggcacgc gtgattgccg
    50281 tgcttgcgac caaaggcagg cacgatcgca ccacttgatt gatataggtc aacctgcaat
    50341 tgatctcagg caagccggca tgctgcacca tcggactgcg gcgtgcacat gcaaagacct
    50401 tgggcatggg ctttcagcgg ccatggattg accgccgcca acaccaaaac caagggtttt
    50461 ccctgaattc gtccgtttac caaaccttga catagttcat gggtcaaccc agcataacag
    50521 tcaagaatcc agggtgctga aaagcctttt aaggagaaga ccatgagcca ccatgacgat
    50581 gagaacgaga gcgttccgct catacagcag ctgctagaca gcccatttct actgctgttt
    50641 ctgggcgtga tgatcccaat ggttgtatac aacctatggg gagttttaga catcctgacc
    50701 attccattgg ccaaataaga ggagccgacc ccatgagcgc aatactccct ccctcagagc
    50761 ggttgtggtg gaaacaccct ctcgaccgtg tcgagggcct ctggatcctc atttcctttg
    50821 tctggtgcat ggtcatgttc ttcatgatgg tgggctggca catctacgga aaacagaacc
    50881 tgtccaccga aacctacaaa accacgcctg agaaatttgc cgcgcaagcg caagcagccg
    50941 tggacaaatg gacggtgcgc actgagctgg agcaaaaaat ccccgtggtg gctccacccg
    51001 agggtgcaga cgtgtacatg atcgcgcgcc tgtggaattt ctggccgatc ctcgagctgg
    51061 agaagggcaa gacctacaag ctgcatctga gctcgatgga ctacaaccac ggcttttctc
    51121 tgcaacccac caacatcaac atccaggtcg tgcccggcta tgagcacgtg atcaaggtca
    51181 cgcccaacga gtccggcact ttttccgttg tctgcaacga gttttgcggc attggccacc
    51241 acgagatggt gggtcgcatc tacgtgaagt aagcggagac accccatgac taccacttac
    51301 cgtacctgcc cgcgcacggg cctgcaattc gaggcccagg ccgagaaact catgattgcc
    51361 aacgccttta tggccgtggt cgccctgctc gtgggcggcc tgctggccat tggcgtggtg
    51421 ctgacccgct ggcccgccgt gcactggctg ccggccgaca ccttctacat ggtgctcacg
    51481 gcccacggca tcgacatgct gatcttctgg atcatcttct ttgaagtcgc agtcctgtac
    51541 ttcgcctcat cgacgctgtt gcgctgtcgc attgccacgc caaaaatagc ttggctgggc
    51601 tttgcgctga tgctcatcgg cgccgtgtgg aacaacatcg tggtgttcca gggcgagtcc
    51661 agcgtgatga tgacctccta tgtgcccatg atggcgcact ggtcgttcta cctgagcctg
    51721 atcctgttcg ccgtgggcgc cctggttgcc tgctttgtgt tcttcggtac gctggtcgtt
    51781 gccaagcgcg acaagaccta cgaaggctca gtcccactgg ttaccttcgg cgccacgacc
    51841 gcagccatca tcgcggtatt caccattctg tcgggcgctg gcgtcctggt gccgaccttc
    51901 ttcgtgtcca tcggactgct ggagagtgtt gacccgctga tgtaccgcgt tgtctggtgg
    51961 gccttcggcc actcgtcgca gcagatcaac gtggcggccc atatctccat ctggtacgcc
    52021 gtggcggcca tcgccttcgg tgccaaaccc atgtccgagc gcgtcagccg tggcgccttc
    52081 ctgctgtaca tcctgttcct gcaactggcc agtgcccacc acatcctcgc cgatcccggc
    52141 gtcagcaccg agtggaaggt ggtcaacacc agctacttca tgtactttgc cgtgctgggt
    52201 tccatgatcc acgccctgtc gatcccgggc gccatggaag tggcgcaacg cgcacgcggc
    52261 tacaacaagg gtttgttcga gtggctgcgc aaggcaccct ggagcaaccc cacgttctcc
    52321 ggcgttttca tcgcactcgt gggcttcggc ttcctgggcg gcactaccgg cgtgatgatg
    52381 ggtaccgaac agctgaacct gctgatccac aacaccatct acgtgcccgg tcacttccac
    52441 gccaccgtgg tggtgggtac cacgctgacc ttcatggcgc tgacctactt cctgatcccc
    52501 tcgctgttcc gccgcgagat gatcgccccg ggtctggcac gtctgcagcc ctatttgttc
    52561 ggcttctcga tgtacttctt ctgcctggtc atgatgggcg cgggtacgct gggtgtgtca
    52621 cgccgtcact gggacatggc cttcagcggc aacgccctgg cctatgaatg gccaggagcg
    52681 gcctacctga tgatgggcct ggtcggtatc agtggcgtcg cagccattgc cggtggtgtt
    52741 atctacgtgt acgtgaccgt gggttcgctg ctgtggggca agaagctcga cgctggcgtg
    52801 accagcccga agttcacccc ggttgcacgt gcggccccga ctgccgccgc gcagacctac
    52861 ggctcggctg gcttcgtggc ccctggcacc tttgtgctgg ccatgttctt cctcgtgacc
    52921 tttgtgctgt actacttcgt gaactggaag tacctcggcc agctgtgggg tctgagctga
    52981 tccggagccc tgcgtcatgc ataccacaac gctcaagccc gcagggcgca gcacggcgca
    53041 gatggcgcgc gacgtcatct cgctgttcaa gctgcgcata ggcgtgctga tcatgattac
    53101 cgcgctcgta ggcatggcgg tgacgccagg gcccgcgccc agcttcgtgc aggcgctggt
    53161 gctggccatc tccgtgctga tggcgtcggc cagtgcagga gcgttcaacc agtatgtgga
    53221 gtttgagagc gaccggctga tgcggcgcac cagtcggcgc gcgttcgcca ccggggccct
    53281 gccccaccac cccgcctggc tggtcttgat ggccgtgctg ctggcggtgg cggtggcatc
    53341 ggcctggctg gtgctcaacg ccgtggcggc cctgcatgtt tttctgggcg cgtttttcta
    53401 cggggtggtc tacaccctgt ggctcaagcg ccgcaccgcc atgaacatcg tcatcggcgg
    53461 tctggccggc agctttgccg tgctggccgg cgccgccgcg gtcgatcccg gcctgggccc
    53521 gctgccctgg ctgctggcac tggtgctgtt cctgtggacg ccgccgcatt tctggagcct
    53581 ggccatcgcc aacagcgctg actatgccca ggcgggcgtt cccatgctgc cggtggtcgt
    53641 agggcccgag cgcgccgcgc gcaccgtgct cctgagtacc atcgccctgt tccttgcctc
    53701 gctggcaccc ctggcctttg gcgcgggatg ggtgtacggc gcaggggccc tcgtgggcgg
    53761 cctgcacttc gtgcgcaagt cctggcagct ggcacgcaag cctgagcgcg ccacggccat
    53821 gggctcgttt tttgcctctc tggttcaact gagcctgctg ctggttgccg ccagcataga
    53881 cgcgctgatg cgctgaaacg gaaagccacg ccatggcctg cctgagtttg cttcgcatac
    53941 gccacgcgct tgccacgtgc gctgcggggc tgatgctggc gctgccggcc atggcctctt
    54001 cctccaacac caccgacctg gggctggatc gcgaagctgc gctgcgcacc agccaggccg
    54061 ccattggccg caaggtcggt gaccatattc tgctcaaccg cgagggcgtt cctacctccc
    54121 tcgcgtccta ccgcggcaag ccgctgctgg tgagtttcat ctataccggt tgcttccagg
    54181 tttgcccgac caccacgcgc tcgctgcaag aagccgtgga gggcctgctc aaaagcgtgg
    54241 ggcccaacca gttcaacgtg gtgagcatag gcttcaacca gccggccgac tcgccacaag
    54301 ccctgaagtc ctttgccacg caatacggca tccgccagcc gaactgggaa tttttaagcc
    54361 cgccggcagc cctcgtgccc gatctggcgc gggatttcgg ctttgtcttt caggcctctc
    54421 cggcaggctt tgaccatgtg ctgcaggttt ccatcctgga tggcgagggg cgcatcgtgc
    54481 gccaggtata tggcgagggc gtcgtcccat ccgagctggg cgagccgctg aaaatgctgc
    54541 tggcgggcca gcctgtcgag tccaacgaac tgatcgagca tctggtggac agggtgcgca
    54601 tcctgtgcac ggtgttcgat cccaagaccg gcacctataa ggtcgattac acgcttccca
    54661 tacaaattgc cggcggcgtg acgtttttcg tcctgatgct gatctttttc atcaacgaat
    54721 ggcgcagcag ccgcaagatg gctcgtagca aggggatgcg caacgcctga tgcgcaacgc
    54781 cagtcccatg tcattgccag gctccgccct gcccgtccag ggccccgcca caaccccgcc
    54841 cccactgccc cgccttgcca gcgcctaccg cacgctggaa cacggccttg acatggcgtt
    54901 tggcgccgca gccaacccgc tgaaacacct gggcgcgctg ggtttcatgc tgctatggct
    54961 gttggtggcc agcggcagca ttctctacat cgtgctggac acttctgccg ccggcgccta
    55021 tcagtccatc gccgcgctcg ccgatgttcc atggatgctg ggcacggccc tgcgcggcgt
    55081 gcaccgctac gcagcagacg ccttcgtcgt cgtcatgggc atgcatatcc tgcgcgagtg
    55141 gctgctgggg cgctaccggc attggcgcag ggcaatctgg tggacgggcg tgcccctggt
    55201 gggcctggcc tttgtcagcg ccgtgggtgg tttctggctc aactgggatc gcctgggcca
    55261 gtattcggtg gtggccaccg ccgattggct cgatgccctg ccgattctgg gcgcctccct
    55321 ggcgcgcaac ttcctgggtg acgtgagcct cagcgaccgg ctattcaccc tgttcatctt
    55381 catccacatc ggcatgccgc tgctgatggt gttcggcctg tggtttcaca tacagcgcat
    55441 cgcgctggcc aaggtatttc caccacgccc gctggcctgg ggcaccttcc tgacactgct
    55501 gctgctcgcc ctgctgctac ccgttcacag ccagggcccg gcagacctgc gcagccagcc
    55561 cacggccctg gcgctggatt ggtggctgct gttcatacac ccgctggttg atgccacctc
    55621 gccgaccacc gtctgggtgt tgctgggcct ggcctttgca ctgctggcgc tgccgccgct
    55681 gtggcctggc cgtgcgcgcg aggccgtggc cgtggtctat cccgaacatt gcagcggctg
    55741 ctcgcgttgc tttgacgact gcccctactc cgccatcacc atggtgccgc atccgaacgc
    55801 caagccgggc atgcgcctgg cacaggtgaa cagcgacctg tgcgccagct gcggcatctg
    55861 cgccggctcc tgcccatcat ccacaccgtt tcgcagcgcc gagcaattgg tgacgggcat
    55921 agacatgccg caatggccgg tcaacgatct gcgcacgcag ctgatccaga gcctgcatgc
    55981 catgcctggc cccgagcgct tcgtggtctt tggctgcgcc cagggcatac actcgcaact
    56041 gcaagcgccc gatgtgctgc atttcaagct ggcatgcgcg ggcgccctgc caccctcctt
    56101 tgtcgaatac gccctgcgcc acggcgccga tggcgtgctg atcaacggct gtcgtgaagg
    56161 cggctgcgaa taccgcctgg ggcaacgctg gacggcgcag cgcctgacag gcgagagaga
    56221 accccatctg cgccgttcgg taccccagga tcgctggcag gtcgcctgga ccgatagcgg
    56281 cgatgaacac aggctggcgg ccgcgctgga cgacatgcgc cagcgcagcc gcacggcaac
    56341 gcaatctgtt tctgaggaga gtcattgatg aaacgcgcac tcaactggac tggccaggcg
    56401 ctcttgtacg gcggctttgc cgtcgcgctg ggcgtgtttt cacgctggcc ggtataccac
    56461 ccactgcccg aggacatggc gcagatcaag gtcagcttca tccaccacgg agcgcgcgtg
    56521 gccgagtgcc gcccctacac ggccgaggag caggccaaga tgccgccgaa catgcgcaag
    56581 gcggagaagt gcgaacggga gcgctcgccc gtgtcgattg aagtggacat cgacggcaag
    56641 accgtgctgg cgcacacctc gccaccctcg ggcctgtcgc gcgatggcgc ctccacgctg
    56701 taccagcgcc tgaacgtgag cgctggccaa caccagatcg ccgtgcgttt caaggacaac
    56761 caaaagtcgg agcagttcac ctcgcagatt gacgagaccg tcaccctcaa gccggcacag
    56821 gtgctggtga ttgactacag caccgacaag ggaggcattg tcttgcaatg agctcccacg
    56881 cctcgcccta tcccgaccgg cctgcaacat cccctgttgc cgagccggca gccgcgcaag
    56941 cgcgcgccaa ctatgagctc agcctgccca acgatgcccg cctgccgctg gcacgcgggt
    57001 ggttgtggct ggggctggcg gcgctgatcg gctcgggcct gttttccatc ctcctggtcg
    57061 cctcacgcac gccctatgtg aaccagtggc tgccttcggg caacttcttc catattgccc
    57121 tggtgctgca tgtggatctg tcggtgctgg tttggtttgt cgcaatggcc ggtctgctat
    57181 ggagcctgta cggccggcca catgcgtctg gcctgggctg gctggcgctg tgggtgacgg
    57241 ggggcggcac cctggccatg gccctggcgc cgttcctgaa cccgggcgag cccgtcatgg
    57301 ccaactacat tccggttctc gagtccccgc tgtttctctc cgggctggtg gtgtttggcc
    57361 tgggcgccac gctgctggtg ctgcgcagcc tgctgaccac accccgcatg gggccgcacc
    57421 tcgatgggca gggcgcactg ggctttggcc tgaacgcagg cggcgtggcc accgccgtgg
    57481 ccctgctgtg ctttgcctgg tcgtggatcg tgttgcccac cagcctgcac ggcaaggcgt
    57541 attacgaaat cctgttctgg ggcggcggcc atgcgctgca gttcacctgg acgctgctca
    57601 tgctcgtcgc ctggctgtgg ctggcgaacg cctgcggcgc gcgcatcgtg ctcagtccac
    57661 gcatcaccgt gctattgctg ctgctggccc tggcgggtgt tttcgtcacc cccgtggcct
    57721 atctggcaca tgacgtgacc tcggtggagc accgcaacct gctgacctgg gccatgcgcc
    57781 tgggcggcgg cccggccatc gtgccggtgg ccctggcggc cgtgctgggc gtgctgactg
    57841 tgcgcctgtc aaacgatgcc gcgctgcgcc cgctgcgcgc ggccctgctg tcctcggtgc
    57901 tgctgtttgc cgccggcggc gtgatcggcg tcttcatcca cggcagcaat gtgcgcattc
    57961 cggcgcacta ccacggcagc atcgtcggcg tgacgctggc cctgatgggc gcggtctacc
    58021 gcatccttcc ggccctgggc taccaggccc cgcaggggcg catggcgacc ttgcagccct
    58081 ggctgtatgg catgggccag ctcatgcaca tcatcggtct ggtctggtct ggcggctatg
    58141 gcgtgcagcg caaggtggcc ggcaccgatc aggtgctgcg cagcacggcc gaagttgccg
    58201 gcatgggcct gatgggcctg ggcgggctga tcgccatcat tggcgggctg ctgtttgtcg
    58261 tcgtcgtgct gcgcgccatg cgccagccgc aagcactgtc ccattgaaga aagcaagcca
    58321 tgatcaaaaa attccaagac ctgctgcagt ggctgtttct gcgcatggag gggctgttca
    58381 acgccgcctt tggcgaccgc atcaacccct tctaccacct gggcgccatc accttctacc
    58441 ttttctggat tgtcggtgcc agcggcctgt acctctatat cttctttgaa accggggtgg
    58501 ccgaggccta ttcctcggtg atagcgctca ccaccaagca gtggtggctg ggcggcatca
    58561 tgcgcagcgt gcaccgttac gcatccgacg ccatggtgct gaccatgatg ctgcacatgc
    58621 tgcgctactt cgccttcaac ctctaccacg gctttcgctg gttctcgtgg gtcaccgggg
    58681 tgatgctgat ctggatggtc tacgcctcgg gcatcaacgg ctacatgctg ccctgggatc
    58741 agctggcgca gtacatcacc gtcaccagct tcgaatggct ggactggctg cccatcttca
    58801 acggcagcct gatgcgcaac ttcctgtatt cagaccatgt gggtgcgcgc ttcttcacgc
    58861 tgctgtcctt catgcacctg ggcattccgc tggtgttgct gatggtgatg tggatccacg
    58921 tacagcgcgt gcccaaggcg cgcaccacgc ccccgggccc catcgtctac gggctgctgg
    58981 ccaccttgct ggtgctctcg ctgatcgcac ccgtgcacag ccagggcggg cagacggatc
    59041 tggcgcaggc cgccacgggc ctgcagcttg actggttcta catggcggtg ttcccgctgc
    59101 taaccgaatg gccgctgggt cgcgtctggg ccctggtgat cggcgtctcg gtcgtggtgt
    59161 cgctgctgcc ctggtggccg ccgcagttca agcgtggcga caagcgcctg catcgggtac
    59221 agatccacgg cgaacaaggc gcgttgagca ccatcaccgt acgcaccgat gagaccattc
    59281 tggacgccgg cctgcgccag ggcctggccc tgccctatga atgccgcaac ggtggctgcg
    59341 gcctgtgtgt ttgctccatc gagcagggcc agtacgagca ccgcccctac cagaagacgg
    59401 ccctgcccga cgccgacaag gaacgcggca aggcgctgat gtgctgcgcc gtgccgcacg
    59461 aagacatggc catcgacgtg gccggcttca ccggcgccac cgccgaggcg atggaaacct
    59521 ttcatgccag cgtggcacag atggagcgcc tgtcgcccga cgtgatgcgc gtcatgctgc
    59581 aactgccggc gggccagcgc ctgctgttcg ccgccggcca gtacatcaac atcctgctgg
    59641 acgatggcca gcgccgcgcg ttctcgtttg ccaaccggcc aggggccagc gaccagatcg
    59701 agctgcatgt gcgcctggtg cccggcggac gctggaccac gcatgtgttc gatggcatga
    59761 agccgggcga caacgtgcgc tttgaaggcc cgctgggcca gttcaccctg cgcgagagcc
    59821 agcaccccat tctgttcatc gccggtgcca cgggctttgc gcccatcaag agcatcgtcg
    59881 aggacgcctt tgcgcgtggc gtgcagcgcc cgatgcgcct gtactggggc gtgcgccagc
    59941 cgcaggactt gtacatgctg cagcaggccg agcagtggca acgcgagcac gacaacttca
    60001 cggtggtgcc ggtggtgtcg gagcccacgc ccgaggacgg ctggagcggg cgcactggcc
    60061 tggtgcacga ggccatgctg gcggacttcc ccagcctgag cggcaacgag gtctatctgt
    60121 gcggctcggt gcgcatggtg gagacggcgg tgccggcctt catcgcccat ggcctcgatg
    60181 agagcttctg cttctccgac gccttcgtga tggccgcgcc cgcaccggag cagcagccac
    60241 ccggggccgc cgccgctacg gcttgagcag atccgccccc gcgggcggct tgaactggaa
    60301 gctgcccgcg ggcagcgccg ggttgagctg catgcccgtg aagcggatct gcgaacgctg
    60361 gccaaagctg tccagaatgt ccagtgcggc cagcttgtcg ccctcgaagc ccacgcgcac
    60421 gctcttgagc tggccgtccc tggcctttgg cgtggcctgc acccactgca ggccatcctg
    60481 ctcgggcgcg gcctcgagcg catagtcggc acgcagcgcc gccatgtccg gcgccgacgc
    60541 cagcagcgcc gcgggcgtgc tgcccagggc ctgtgcctgg gcgcgctgcg tgacctggtt
    60601 caggtccacg tcgtagagcc acagcgtctg gccatcggcg actatggtct gctcgaaggg
    60661 cttctggtag acgaacttga agcgccccgg gcgctggaac gcaaagctgc cgctcgattg
    60721 cttgggccgc tgtgcctggc catccttcgg gggcgccgtc acgatctgag tgaagtcggc
    60781 ccggccgctg tgggcggttt tcatgaagtt ttcaagactt ttcaggccgt cagcgcttgc
    60841 ccattgtgcg ctagcagcta tcaaaattgc agcaaccgct ttcttcacaa ccccacctcc
    60901 tactcgctgc gcgcgggcac cagcacctcg cgctggccgc tgctggtcag gccgctgacc
    60961 aggccggcct tctccatatc ctcgagcagc cgcgccgagc ggttgtagcc aatgcgcagc
    61021 ttgcgctgca cgtaggagat gctggccttg cggtctttca gcaccacctc cacggcctgg
    61081 tcgtacatcg gatccttctc gccgccgccc tcgccatcgt ccaggccaaa gccgctgtcc
    61141 tcgccatcgg cgctgccgcc ttccaacacc ccttcgatgt aatcgggctc gccttgttcc
    61201 ttgaggtagc tgacgacgcg gtgcacctcc tcgtcactga cgaaggcgcc gtgcacgcgc
    61261 accggcagcc ccgtgccgct ggccatgtag agcatgtcac ccatgcccag cagtgcctcg
    61321 gcgcccatct ggtccaggat cgtgcggctg tcgatcttgc tgcccaccga gaaggcgatg
    61381 cgcgtgggga tgttggcctt gatcaggccg gtgatcacgt ccacgctagg gcgctgcgtg
    61441 gccaggatca aatggatgcc ggcggcgcgc gccttttgcg ccaggcgcgc gatcagctcc
    61501 tcgatcttct tgccgaccac catcattagg tcggccagct cgtcgatgac gacgacgatg
    61561 tgcggcaggc gctccaacgg ctcgggcgcc tcggggttga gactgaaggg gttgggaatg
    61621 gactcctcgc gtgccttggc ctcgtctatt tttgtgttgt agcccgccag gttgcgcacg
    61681 ccgagtttgc tcatcagctt gtagcgccgc tccatctcgg cgacgcacca gttcaggcca
    61741 tgcgcggcct gcttcatgtc ggtgaccacc gggcacagca ggtgcggaat gccctcgtag
    61801 accgacattt ccagcatctt cgggtcgatc atcaagagcc gcacgtcgcg cgcctcggcc
    61861 ttgtagagca ggctgagaat catggcgttg atgcccaccg acttgcccga gcccgtggtg
    61921 ccggccacca gcacatgcgg catcttcgcc aggtcggcca ccacgggcgc gccgacgatg
    61981 tctttcccta agcccatggt caagaggctc ttggcgtcgt ggtagacctg cgagcccagg
    62041 atttccgtca ggcggatcgt ctggcgcttg gcgttgggca attcgagggc catgaagttc
    62101 ttgcccggaa tggtctcgat cacgcggatc gacaccagcg acagcgagcg cgccagatcc
    62161 ttggccaggt tcacgatctg cgagcccttg acgcccgtgg ccggctcgat ctcgtagcgg
    62221 gtgatcaccg ggccgggttg ggccgccacc accgtcacgt ccacgccgaa gtccttgagc
    62281 ttcttctcga tcaggcggct ggtcatctcc agcgtctcgg gcgacaccgt ctcctggcgc
    62341 gcctgggccg catccagcaa gtccacctgc ggcaatcggc tgtcgggcat gtcgctgaac
    62401 aggggctttt gccgctcctt gaccacgcgc gtgctctgcg gcgcatcctg cagcacgggc
    62461 tcgatgatgc gcacgggctg cggatgctgc acctcgctct cgtgccgctc ttcctgcacc
    62521 acctcctcgc gctcgcgcgc ggcgcgtctg cccacggcca catccttggc cctctcgcgc
    62581 tgggcacggc cggtttgcac cagcgcatcg atgcgcgcac ccaggcgctc ggccagatgc
    62641 ccccaggaaa agcggaacac cagcgccgcg cccagcacca ccagcaccac gccggccagg
    62701 cccgagcccg caaagcccag ccacttcacg gccagcggcc ccatcacata gcccagcatg
    62761 cccccggcat gcccgggcag caacgcctcg aaacgataca ggcgcgacca ctccaacgcc
    62821 gtgctggtgc acatcagcag caacaacccg ccccagaaca tggcgcgcca cagccagggg
    62881 ccatgggccg ttgcacccgg cacgggctca ccaccacgca tccagcgcgc cagcgacgac
    62941 agccaggcgc gcacccccgc ggccacgcac caccagacgg agaaaccgaa cagaaaatag
    63001 ctgccatcgg ccagccaggc accaaagcgc cccacccagt tggccagggg gcggccgccg
    63061 cccgcgccgg acgtggacca ggccgcgtcc tgcgcggaat agctgaccat ggccagcagc
    63121 cagaacacca gggccaacag gcccaggaca aggccaagtt catgcccaaa gcgcgccgcg
    63181 gccgagcgtg gcgcagactt tccggcgccg ttcaaggtat tgagggagta ggtcaaaaga
    63241 tcgcgaacac gttcaacaac agcgtgcaag cttaccccat ggcgcaaggc tgcaagccgc
    63301 cgtgcacccc acgccaagcc gacgcaagag ccagcatgag agccggcatg cacccaccgg
    63361 gctgacgtga tcgacggctt catacaatgg ctgcgctttt gccctttttc tgccctttcg
    63421 tgcccgctgc acgggccgcc aacacccctt cggttccgcc atgtccacta cccaacacgc
    63481 caaagtcctg atcctcggct ccggcccggc cggctacacc gccgccatct acgccgcgcg
    63541 cgccaacctg aatccggtgc tgatcaccgg catggcccag ggcggccagc tgaccaccac
    63601 caccgaggtg gacaactggc cggccgacgt gaacggcgtg cagggcccgg agctgatgca
    63661 gcgctttctc gagcatgccg agcgcttcaa gacgcagatc gtgttcgatc acatcaacgc
    63721 cgtcgatctg tccaggcgcc cattcaccct gaccggcgac agtggccagt acacctgcga
    63781 ctcgctgatc atcgccaccg gcgcgtccgc caagtacctg ggcctgccgt ccgaggaggc
    63841 cttcatgggc aagggcgtgt ccgcctgcgc cacctgcgac ggcttcttct accgcgagca
    63901 ggaggtctgc gtggtcggcg gtggcaacac cgcggtggaa gaagccctgt acctggccaa
    63961 tatcgcatcc aaggtcacgc tagtgcaccg gcgcgacagg ttccgcgccg agcccatcct
    64021 ggtggacaag ctgatggagc gcgtggccgc cggcaagatc gtcctcaaga ccttcttcac
    64081 gctcgacgag gtgctgggcg acgccagcgg cgtgaccggg atccgcatca agagcaccga
    64141 agacgggcat accgaggaca tcaagctcca gggctgcttc atcgctattg gccatgcgcc
    64201 caacaccgag atcttccagg gtcagatcac catggaaaat ggctacatcg cgacccaggg
    64261 cggcctcaag ggctttgcca cgcagaccag cgtgcccggc gtgtttgccg ccggcgacgt
    64321 gcaggaccat gtgtaccgcc aggccatcac cagcgccggc acgggctgca tggcggcgct
    64381 ggatgcccag cgctatctgg aacagcagga gtgatctttc aggctcgcgc tataatgcgc
    64441 ggctttgctg tattgtggcg ccccggcgcc ttgagacagc cgggttaccg ccacccgatt
    64501 caactggcga ggtgaggtta cgggccgcca gggcctgcag ccgtgcaact accggagtgt
    64561 cctgatggca cgcgtatgtg aagtaacggg caagaagccc atggtgggaa acaacgtctc
    64621 ccacgccaac aacaagacca agcgtcgttt cctgcccaac ctgcagtacc gccgtttctg
    64681 ggtggagagc gaaaaccgct gggtgcgcct gcgtgtctcc agcgccgccc tgcgtctgat
    64741 cgacaagaac ggcatcgaat ccgtgctcgc agacatgcgt gcccgtggcc aagcttaatt
    64801 tcctgaagga gaaacaccat ggctgctaaa ggcggacgcg aaaagatcaa gctggtgtcc
    64861 acggctgaga ccgggcactt ctacaccacc accaagaaca agaagacgat gcccgagaag
    64921 atgagcatca tcaagttcga ccccaaggct cgcaagcatg tcgagtacaa ggaagccaag
    64981 ctgaagtaat tcggcccggc tcctgacaag aaccgccacg gcaccagccc tggcggtttt
    65041 ttttggccta tttgcgctgc cacacggcag cgaagaagcc gtctgtctgg tgcagatgcg
    65101 gccacaggcg caggtaggcc tgaccgtccg caccgccgct gcacaggctg gccgcctgct
    65161 ccaccttgag ccgcgccagc agctcgcccg ccgccagcgg ctcgaaatct gcgtgggcgg
    65221 cgccaaaggc ctgggcgatg gcctcgttct cctcgggcag cacgctgcag gtggcataaa
    65281 ccaggcggcc gccaggcttc accaggcgcg cggcactctc caggatagcg gtctgcttgg
    65341 ccgtcagctc ctgcacggcc tggggcgact ggcgccattt cagatccgga ttgcggcgca
    65401 gagtgcccag acccgagcag ggcgcatcaa ccagcacgcg gtcgatcttg ccggccaggc
    65461 gcttgacgcg ctcatcgcgt tcatgggcga tggccgccgg gtgcacattc gacaggccgc
    65521 tgcgcgccag gcgcggcttc agcgcatcga gccggtgcgc cgacacgtcg aacgcataca
    65581 gccgcccggt gctgcgcatg gccgcgccaa tcgccagggt cttgccgccg gcaccggcac
    65641 aaaaatcgac caccatctcg ccgcgcttgg catccagcag cagggccagc agctgcgagc
    65701 cctcgtcctg cacctcgatg gcgccgcgcg tgaaggcgtc cagccgggtc agcgccggct
    65761 tgccctcgat gcgcaaaccc cagggggaat acggcgttgc cttggccttg atgccggcct
    65821 gcgccagctc cttttgaacc gattcgcgct tgtccttcag ggcgttgacg cgcagatcca
    65881 gcggtgccga ctgccccagg ctgtcaacca gcggccagaa accatcgccc agctgggcct
    65941 tcagcggctc ggccagccac tgcggcaggt tgtggcgctg cggctccagc agctcctcgg
    66001 ggcgcaccgc gtcgcaggca tcgacccagt ccttttcctg cgcggagagc gcgcttttca
    66061 gaaagtcgcg cgggccgtaa aagcccagaa tggccaggcg ccgctcacgc gggccttgac
    66121 ctgagcgcgc cagctgctcg aacagcggct tcttgcgcag cacggtgtag gcggtttcgg
    66181 ccagcgtggc gcgctcgcgc ggcccgagac tgcggtgttc gcggaaatag cgcgagacga
    66241 cagcgtcggc cgggtgttca aaagtaagcg tcagcctgac gagttcggcg caggcgtcca
    66301 gaagggcttt gggatgcatg ggtgtgattg tcccatcttg cggcgctgtc tcgccggccg
    66361 catcacttat caaaaaaact ggccttagcc tttatccatc aagcaccaac agctatattt
    66421 ttgaaagcag atgcgccaac accgccctgg ggtaaatcag gtgctcctgt gtcagcacgc
    66481 gcgccgccag actctcggcc gtgtcatcag ggagcagcgg caccacggcc tggtccagga
    66541 tcgggcccac gtcgagctcg gccgtcacca ggtgcacggt gcagccggca aacctgcagc
    66601 cggcatcaat ggcgcgctgg tgcgtgtgca ggccggtaaa agccggcagc agcgaggggt
    66661 ggatgttgac cagccggccc gcaaagtgat cgacaaagcc cggcgtgagg atgcgcatga
    66721 agcccgccag caccaccagg gccggctcgt aggcatcaat cgcctgggcc agggcggcgt
    66781 cgaaggcctc gcgcgtggca taggccttgt ggtccagcac ctgggtggct atgccctgct
    66841 cgcgtgccag ccccaggccc ttggcgtccg ccttgttgct gacgacggcc gccacgcgta
    66901 tgccgtggcg ccccgcccag tcctgctgct gcgccgcgcg caccatggcc gccatgttgg
    66961 agccgccgcc agagatcaaa atcacgatgt ttttcattac ccgccaatta tccccgccat
    67021 gcccttcgac ccacgacaac gccccctgac cccggccgag gcccgcgtgc tcgccacgct
    67081 cttggaaaag tcgcgcaccg tgcccgacag ctaccnnnnn nnnnnnnnnn nnnnnnnnnn
    67141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    67201 nnnnnnnnnn nnnnngcccg cgtgctcgcc acgctcttgg aaaagtcgcg caccgtgccc
    67261 gacagctacc cgctaacgct caacagcctg gccgccggct gcaaccagaa gaccagccgc
    67321 gagcctgtca tgcagctggc cgacgacgac ctgctgcagg cactgaccag cctgcgccag
    67381 cagagcctgg tggtcgagat aggcggcgcc cgcgtggcgc gctgggagca caattttgcg
    67441 cgcggcatag gcgtgcccga tcagtccgcc gtgctgctgg gtctgctcat gctgcgcggc
    67501 ccgcagaccg ccgccgagct gcgcctgaac gccgagcgct ggcaccgctt tgccgacacc
    67561 tcgtcagtcg aggctttcct cgacgaactg caggagcgca gtgacgaaaa gggcggcccg
    67621 ctggtcatgc tgctgcccaa ggcgccgggc gcgcgcgagc cgcgctgggc gcagctcttg
    67681 tgcgggccgg tggacgtggc ggcgctggcc gccagccagg cagcaagcgc cgcgccagcc
    67741 cagccggctc tgcaggcgcg cgtggaggcg ctggaggccg aggtggccca gctgcaggcc
    67801 caggtgcgcc gcctgtgcgc agagctgggc ctggacgcac cgctgtcgcc gcccggacag
    67861 gagtgaggcg ggctgccgtg ctacaaccgc ctcctgcaaa ggagacgttc gcatggatct
    67921 cgcattcacc cccgaggaac aggcctttcg ccaagaagtg cgcgcctggg tgcgcgccaa
    67981 tctgccgcca gcgatggcgc acaaggtgca caacgccctg cgcctgacga gggacgacct
    68041 gcagggctgg gccaggatac tgggcaagaa gggctggctg gcctttggct ggcccaagga
    68101 gtttggcggc cccggctgga cggccgtgca aaagcatttg ttcgaggagg aatgcgccat
    68161 ggccggcgcg ccgcgcatca tccccttcgg gccggtgatg gtggcgccgg tcatcatggc
    68221 ctacggcacg cccgagcaac aaaaacgctt cctgcccggc attgccgatg gctccgtgtg
    68281 gtggagccag ggctactccg agccgggctc gggctcggat ctggccagcg tcaagacccg
    68341 cgccgagcgc gtcggggata aatacatcgt caacggccag aagacctgga ccacgctggg
    68401 ccagcatggc gactggatgt tcaacctggt gcgcaccagc aacgagggca agccccagac
    68461 cggcatcagc tttttgctgc tggacatgaa gagccccggc gtgacggtgc gccccatcaa
    68521 gctgctcgat ggcgaatgcg aggtcaacga agtcttcttc gacaacgtcg aggtgcccgc
    68581 cgaccagctc ataggccagg agaacaaggg ctggacctac gccaagcatc tgctgtccca
    68641 cgagcgcacc aacatcgccg acgtaaaccg cgccaagcgc gaactggagc gcttgaagcg
    68701 catcgcccgc gccgaaggcc tatgggacga ccagcgtttt cgcgaccaga tcgccctgtt
    68761 ggaggtggac atcgtggcac tggagatgct ggtgctgcgc gtgctgtcga tggaaaagag
    68821 cggcaagaac ccgctggaca tcgccggcct cttgaagatc aagggcagcg agatccagca
    68881 gcgctacgcc gagctgatga tgctggccgc cggcccctac gccctgcccc tgatccagga
    68941 ggccatggac gcgggctggc agggactgtt ccccggcggc accctggcca acgcgccgct
    69001 ggcatcgacc tacttcaaca tgcgcaagac caccatctac ggcggcagca atgaagtgca
    69061 acgcaatatc gttgcccaaa ccatcctggg ctgacaggag acaagcacat ggacttcgat
    69121 ttttccgacg accagcaaca actgcgtgac gccgtgcgcc gctgggtgga caagggctat
    69181 accttcgagc gccgccgcgc catcgtggca gccgggggct ttgagcgcgc ggcctatggc
    69241 gagctggccg agctgggcct cacggccctg accgtacccg aggcacatgg cggcctgggc
    69301 cagggcgcga tcgacgccat ggtggtgatg gaagagctgg gccgcggcat cgtgctggaa
    69361 cccctggcgc aggccttcgt caccgccggc gtgctggcgc agtacgcacc gcaagagctg
    69421 caggccgcct ggctgccgcg catcgccagc ggcgcggtcc tggtggtgct ggcacagcag
    69481 gagcgcaagg cgcgctaccg ccttgatgtt tgcgaggcaa aagcggtcgt agcgcccaat
    69541 ggatatgcga ttacagctat aaaaaacata gtttcagccg gtgaccaggc cgacgccttc
    69601 atcgtccccg cgcaactcga cgggcagatc gccctgttcc tggtggagcg cgctgccgcc
    69661 gacgtcagca cgcgcggcta cggcacccag gacggcagcc gcgccgccga cctgcagctg
    69721 gccagcaccc cggccaccct catcaccacc gacggcctgg ccgcgctgga gttggcggtg
    69781 gacaccgcca tcgccgccgc ctgcgcctac gccgtgggag tgatggatca gacctgcgcc
    69841 gtcaccttcg actacctgaa ccagcgccgg cagtttggcg tggcaatcgc cagcttccag
    69901 gccctgcgcc accgggcggc cgacatgaag atgcagctgg agctggcacg ctccatgagc
    69961 tactacgcca gcctgaaact cggcgcccct gccgaggagc gccgccgcgc actgtcgcgc
    70021 gccaaggtgc agctgggcca gtccatgcgc ttcgtcggcc agcaggcggt gcagctgtcg
    70081 ggtggcatag gcgtgacgga cgagtacatc gtcagccact acttcaagac cttgacgcag
    70141 ctggagatga gctggggcga cacgctgcac cacctgggcg aggtgtcggc gcgcatgcag
    70201 gagcaggcgg gggtgtttgc ctga
//
LOCUS       DAOMTQ010000003        59091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_19_length_59091_cov_26.797530, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000003 DAOMTQ010000000
VERSION     DAOMTQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 59091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59091
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_59091_cov_26.797530"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1621)
                     /gene="guaA"
                     /locus_tag="PK518_00690"
     CDS             complement(5..1621)
                     /gene="guaA"
                     /locus_tag="PK518_00690"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRM47456.1"
                     /translation="MAHDKILILDFGSQVTQLIARRVREAHVYCEVHPCDVSSDWVRE
                     FAADGRLKGVILSGSHASVYEVDDKAPDAVFELGIPVLGICYGMQTMANQLGGKVEGS
                     NSREFGYAEVRAHGHTALLKGIEDFATAEGHGMLKVWMSHGDKVTALPPGFKLMASTP
                     SCPIAGMADEARGYYAVQFHPEVTHTVQGMALLSRFVRDICGAQADWVMKDHIEEAVA
                     KIREQVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLHEGDMVMDMF
                     QGKLHAKVIRVDASDLFLGKLAGVSEPEQKRKIIGGLFVDVFKAEAAKLKAANAGHKG
                     ATFLAQGTIYPDVIESGGAKSKKAVTIKSHHNVGGLPEQLGLKLLEPLRDLFKDEVRE
                     LGVALGLPREMVYRHPFPGPGLGVRILGEVKKDYADLLRRADAIFIEELRNFTDEATG
                     KTWYDLTSQAFTVFLPVKSVGVMGDGRTYDYVVALRAVQTSDFMTADWAELPYSLLKR
                     VSGRIINEVRGINRVTYDVSSKPPATIEWE"
     gene            complement(1700..3175)
                     /gene="guaB"
                     /locus_tag="PK518_00695"
     CDS             complement(1700..3175)
                     /gene="guaB"
                     /locus_tag="PK518_00695"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRM47457.1"
                     /translation="MRLLGKALTFDDVLLVPAYSQVLPKDTSLATRFTRNITLNLPLV
                     SAAMDTVTEARLAIAIAQEGGIGVIHKNMTAEQQAAEVSKVKRHESGVVHDPVVITPE
                     HTVLQVLELSENLGISGFPVCDGGKVIGIVTSRDVRFETRYDVKVSQIMTPREKLITV
                     NERDHTSPAQAKALLNKHKLERLLVVNDAFELKGLITVKDINKQTTFPNAARDSEGRL
                     RVAAAVGVGAGTEERVAALVKAGVDAIVVDTAHGHSKGVIDRVRWVKQNYPQVDVIGG
                     NIATGAAALALVEAGADAVKVGIGPGSICTTRIVAGVGVPQIMAIDSVAQALKGTGVP
                     LIADGGIRFSGDIAKAIAAGASTIMMGGMFAGTEEAPGEVILYQGRSYKSYRGMGSIG
                     AMQQGSADRYFQEATTGNPNADKLVPEGIEGRVPYKGSMVSIVFQMAGGVRAAMGYCG
                     CATIAEMNDKAEFVEITAAGIRESHVHDVQITKEAPNYRAD"
     gene            3283..3789
                     /locus_tag="PK518_00700"
     CDS             3283..3789
                     /locus_tag="PK518_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRM47458.1"
                     /translation="MKPHKLLLLALACTWATGAFAQWQWLDKDGRKVFSDRPPPSEVP
                     EKNILKQPHISSRPRPAAPPVGAEANADTPVTAQATPGQGKDKQLEENKAKAEAAEAA
                     KKKAEQERVAKARADNCARARLNKVQLAPGRLVAHTNAKGERSYMDDATREAELRRAD
                     DIIAADCQ"
     gene            complement(3817..4155)
                     /locus_tag="PK518_00705"
     CDS             complement(3817..4155)
                     /locus_tag="PK518_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HRM47459.1"
                     /translation="MAEGGAASLRITVAASLRPGEVQEWQLQLAQGATVADALRACGL
                     MPQADRLACGIWGRPAKAHEVLRDGDRLECCRPLTVDPKLARRQRFKAQGARVAGLFA
                     KRRPGSKAGY"
     gene            complement(4148..4588)
                     /locus_tag="PK518_00710"
     CDS             complement(4148..4588)
                     /locus_tag="PK518_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRM47460.1"
                     /translation="MKTVNKSVLIWYSPEEMFALVTDVASYPEFLPWCDHSTVLEQDA
                     HGMKAEVGISLGGLRKSFVTRNTHEQGRRVKMELVEGPFSKLDGDWHFHPVGDGTQRA
                     CKVELQLHYGFDSRTLAALVGPVFDRIAATFVDAFIKRAEDVYG"
     gene            4677..5150
                     /gene="smpB"
                     /locus_tag="PK518_00715"
     CDS             4677..5150
                     /gene="smpB"
                     /locus_tag="PK518_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRM47461.1"
                     /translation="MAKKPDTSSRIADNKKAAYNYFFEERHEAGMVLHGWEVKALREG
                     KVQLTDGYVVIKDGELFLIGCQINPLKTASTHVNPDATRIKKLLLHKEEIRRLIGKVE
                     QKGYTLVPLNLHWKDGRAKCDIALAKGKAEHDKRDTIKDREGKREVERAMKSRHR"
     gene            5252..5617
                     /locus_tag="PK518_00720"
     CDS             5252..5617
                     /locus_tag="PK518_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47462.1"
                     /translation="MTKLNSLLAVLALAGCYSMPHAQVMTQGGALVGANGMTLYTFDK
                     DMAGSGKSVCNGACATNWPPLTATAAPGGEGYSLVTRDDGTKQVAYKGKPLYYWSKDM
                     KPGDKTGDGVNNLWRTATP"
     gene            5624..6148
                     /locus_tag="PK518_00725"
     CDS             5624..6148
                     /locus_tag="PK518_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM47463.1"
                     /translation="MNRAQVVEQLPGLRRYARALTGNAWAADDLVQDTLERACRKWLL
                     WRSGSDLRAWLFTLMHNLYLNQRRGLLEVQPLDDQALATLAGTDDAAADMAMDLDRCL
                     QRLPAEQRAVLLLVTLEDMAYADVARVLDIPVGTVMSRLSRARSRMRELMETAPAQAP
                     AAGAAPPAPLRRMK"
     gene            6172..6999
                     /locus_tag="PK518_00730"
     CDS             6172..6999
                     /locus_tag="PK518_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRM47464.1"
                     /translation="MNTSSSPQDPDASRLHALVDGRLPPEQAALLRASLDAATGEDAD
                     AWARQRALLRTLHGDWLQRPLPEALHQAAERLQNQHAQRRRWATWSGVAAGWVLAFGL
                     GWGLHGASGGGATQAMAAPRLFAQQATVAHAVYQPEQRHPVEVTAAQQEHLVQWLSRR
                     LERPLTVPHLQDQGYELVGGRLLPGGSGARAQFMYQNAAGQRITLYLGALEQAQAEAF
                     HFYGDGAVSSFYWVDRGFGYALSGALPRPALQALATAVYQQLSAAPPAGARAPARGS"
     gene            complement(6950..8041)
                     /locus_tag="PK518_00735"
     CDS             complement(6950..8041)
                     /locus_tag="PK518_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRM47465.1"
                     /translation="MTETTHEVIAELRGQVGCITLNRPRALNALSLGMVRELMAVLLD
                     WQKNDQVLAVAIRGSNKEGAFGAFCAGGDIRFLHQAGTVGNPQLEDFFTEEYALNHLI
                     HNFGKPYIAFMDGIVMGGGMGISQGASLRVVTERTKMAMPETIIGLFPDVGGGYFLSR
                     CPGRVGEWLALSGETIGAADAIAFKLADGFLPADQQAGLWDALGAQSFASGAAVQQWV
                     ESKMIASGACQVSARGQIDQYFSLPDVPTIVAALEGAGDDWARATAALLRKRSPLMLH
                     VVLEQIRRARTMDLADDLRMERDMVRHCFYLRPGQSETLEGIRALAVDKDQSPRWNPA
                     RIEDVAPELVQQFFKSPWPAHAHPLAALR"
     gene            complement(8038..8877)
                     /locus_tag="PK518_00740"
     CDS             complement(8038..8877)
                     /locus_tag="PK518_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM47466.1"
                     /translation="MALAALMWSIAGVVTRHLEQARSFEVTFWRSFFMLLSLLVILPW
                     LSGRDVFAKVWRGPRALWLSGLCWSVMFAAFMVAITLMPVANVLVTMAVGPMLTALVA
                     RVFIGHRIAPRTAAAIVVAGLGIVWMYGAEVRSLSPVGLAVALCIPVAAAINWTVVQH
                     SQRHGQAVDLVPAVLIGAALSAAATLPWALPFQASARDLSLLGLLGLVQLAIPCVLVV
                     YCGRVLKAPEIALLGLLEVIFGIVLVWVGAGERPAPAVLMGGTLVIGALVFNELLGWK
                     EQP"
     gene            complement(8933..10762)
                     /gene="typA"
                     /locus_tag="PK518_00745"
     CDS             complement(8933..10762)
                     /gene="typA"
                     /locus_tag="PK518_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRM47467.1"
                     /translation="MSKQIRNIAIIAHVDHGKTTMVDQLLRQSGTFAQHEKVVDTVMD
                     SNAIERERGITILAKNCAVSWEGTHINILDTPGHADFGGEVERALSMVDGVVLLIDAQ
                     EGPMPQTRFVTKKALALGLKPIVVVNKVDKPGARPDYVVNAAFDLFDKLGATDEQLDF
                     PVVYASGINGWSALEQGNPGEQWGPDMSALFNTVLKHVPSIKGDPAAPVQMQVSALDY
                     STFVGRIGVGRITQGTLKAGQDVLVMAGPEGNSYKGRINQIHQFQGLDRVQVTEAGPS
                     EIVLINGIENVGIGETITDVADPQPLPMLKIDEPTLTMNFCVNTSPLAGREGKFVTSR
                     QIWDRLQKELRSNVALRVKETDEDGIFEVSGRGELHLTILLEEMRREGYELAVSKPRV
                     VFKDIDGERCEPIELVTADIEEGHQGGVMQALGERKGELVNMEPDGRGRVRLEYRIPA
                     RGLIGFTNEFLNLTRGSGLISNIFDSYEPHKGEIGGRKNGVLISMDDGEIFTYALGKL
                     DDRGRMFVKANDPVYEGMIVGIHNRDNDLVVNATRTKQLTNFRVSGKEDAIKITPPID
                     LTLEYGVEFIEDDELVEITPKSIRLRKRHLKEHERKRASREGA"
     gene            complement(10789..11760)
                     /gene="truB"
                     /locus_tag="PK518_00750"
     CDS             complement(10789..11760)
                     /gene="truB"
                     /locus_tag="PK518_00750"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRM47468.1"
                     /translation="MIERAPRIRVQRRPVHGVLLLDKPLGLSSNNALQKVKWLLRAEK
                     AGHTGTLDPLATGVLPLCFGAATKFSALQLDAPKTYEAVALLGITTSTGDAEGEVLQR
                     RDVDPGQLTPERLAQVQAQFTGAIRQVPPMHSALKKDGKALYEYARAGVEVERAPRDV
                     TIHALELAVSQVGKAQTAIKIIVTCSKGTYIRTLGEDIGQALGCGAHLTFLRRIDTGG
                     LGVQRCVTLAALEAMTEDERLACVQSVDTLLAQHERVTLDESEAGRFLSGLRRRGPWA
                     DAPAVAVYGTRPRALLGVAHVAQGELIPDRLLSPLEIQQILESAPRA"
     gene            complement(11753..12130)
                     /gene="rbfA"
                     /locus_tag="PK518_00755"
     CDS             complement(11753..12130)
                     /gene="rbfA"
                     /locus_tag="PK518_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRM47469.1"
                     /translation="MPKRSTKPNRGHQVSDQIQRDLAELIRELKDPRIGMVTLQAVEV
                     TPDYAHAKVFFSVLVGDAEATQEALNQAAGFLRNGLFKRLHIHTVPTLHFLFDRTPER
                     AADMNALIARAVASRSKDDDGND"
     gene            complement(12202..15021)
                     /gene="infB"
                     /locus_tag="PK518_00760"
     CDS             complement(12202..15021)
                     /gene="infB"
                     /locus_tag="PK518_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRM47470.1"
                     /translation="MSSNTVAEFAAELKKSPETLLDQLQSAGVAKSSSADVLNEADKQ
                     KLLAYLQASHGTAGDRKKITLVKKSTSEIKQADATGKARTIQVEVRKKRTFVKREDGV
                     DAAPDPAAVARIAQEQELARREEEARRQAELIRRQEEELAQSRAEREAREQREREAEE
                     RAAAYVAEQEQKKAEEKAGRLEAKEQAVAEAQARAEAQAEARAKAEEESKARAAEEAA
                     RAVDLDERRRKALAEAEAIRAMMAAPKKVLVAKKPEEPKPAAKAADAKDAKKGTLHKP
                     AAGAGARAAAPAAPGAGKEVKSAKLSSSWANDATKKKEIKTRGDSSGGVGRGNWRSGP
                     KGRRGNDRDERQQQQAPAEFRAIEVHVPETITVAELAHKMAIKASEVIKALMKMGQMV
                     TINQPLDQDTAMIVVEEMGHKAVVAALDDPEAFTAEEVSGQQAEALPRAPVVTVMGHV
                     DHGKTSLLDYIRRSRVAVGEAGGITQHIGAYHVETPRGIVTFLDTPGHEAFTAMRARG
                     AQATDIVILVCAADDGVMPQTREAIKHAKAAGVPIVVAITKADKHEANPEKVKQELVA
                     EEVVPEEYGGDSPFVAVSSKTGMGIDDLLEQVLLQAEVLELKAPVEAMAKGLVIEGQL
                     DKGRGPVATVLVQSGTLKVGDVVLAGQTFGRVRAMVDENGQTAKEAGPSIPVEIQGLS
                     EVPQAGDEFMVMADERRAREIATYRAGKFRHTKLAKQQAAKMENVFAEMQAGEVQHLP
                     IIIKADAQGSQEALAASLLKLSTDEVRVQLVYAGVGGISESDVNLAIASKALIIGFNV
                     RADANARKHAEASDVTLHYYNIIYDAVDELKAAMSGMLAPEQREEALGTAEIRTVFVA
                     TKIGTIAGSYITSGQVTRGCRFRLLRENVVIYTGEVEAVRRFKDDVKEVKEGFECGIK
                     LRNYTDIKEGDQLELFEIKEIARTL"
     gene            complement(15046..16530)
                     /gene="nusA"
                     /locus_tag="PK518_00765"
     CDS             complement(15046..16530)
                     /gene="nusA"
                     /locus_tag="PK518_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRM47471.1"
                     /translation="MNRELLMLVEAISREKNVEHDVVMGAVESALAQATKKLYQGEVD
                     IRVSIDRDSGEYETFRRWLVVPDEAGLQNPDAEELLMDARERIADIEVGEYIEEQVES
                     VPIGRIGAMAAKQVILQKIRDAEREMLLSDFMSRGEKIFTGTVKRMDKGDLIVESGRV
                     EGRLRRGEMIPKENLRNGDRVRAMIMDVDLTLRGAPIILSRSAPEFMMELFRNEVPEI
                     EQGLLEIKSCARDAGSRAKIAVLSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIV
                     LWSDDPAQFVIGALAPANVSSIVVDEEKHAMDVVVDEENLAIAIGRGGQNVRLAADLT
                     GWKINIMDAAESAQKQADESDAARRLFMEKLDVDEEIADILIAEGFESLEEVAYVPLQ
                     EMLEIESFDEDTVNELRSRAKDALLTMEIAREESMGAVSQDLRDLEGLTPELIAKLAA
                     GGVNTRDDLADLGLDELTELTGQSAEDATALIMKAREHWFTGQE"
     gene            complement(16600..17190)
                     /gene="rimP"
                     /locus_tag="PK518_00770"
     CDS             complement(16600..17190)
                     /gene="rimP"
                     /locus_tag="PK518_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRM47472.1"
                     /translation="MALQQIVEQTVAGLGYDLVEIERSAGGLLRITIDLVWAAPAAEV
                     QEPVVEQFITVEDCEKVTRQLQYALEVEGVDYKRLEVSSPGIDRLLRNEQDFMRFEGE
                     MVDITLKEPLGAAAGGQVAANRKKFRGTLERVDGGGWQIVWSDAPPVKPGQRVSKKRV
                     PAPLQALGFTLDELREARLAPIVDFKGRGAKPDSAD"
     gene            complement(17384..17755)
                     /locus_tag="PK518_00775"
     CDS             complement(17384..17755)
                     /locus_tag="PK518_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM47473.1"
                     /translation="MQDTESAAGVCQPWWVVCLCAQWCGVCREYRSAFDALARDWPQV
                     RFEWVDVEDEEDLMGDVDVETFPTVLIADGGAARFLGTVLPQTQVLGRMLQSLQADPD
                     APEANAQAQALFERIAASRVR"
     gene            complement(17791..19077)
                     /locus_tag="PK518_00780"
     CDS             complement(17791..19077)
                     /locus_tag="PK518_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRM47474.1"
                     /translation="MGRAWLVWGAGATLLILLAATALVALNRLDELPLPEREAAAAPA
                     SPGLIAQGEYLVRVGNCMSCHTQPGGPAFAGGRAIATPFGSVYAPNLTPDDATGLGRW
                     SAAEFWRAMHNGRSRDGRLLYPAFPYPSYTYITRADSDAIYAFLRSLSPVPLQSPPHV
                     LRFPFNTQWALAGWRALFFRPGTFVQQSTQSAEWNRGAYLVQGLGHCAACHTPRNALG
                     ALRTDAQFHGGLIPAQNWYAPALNSGREAAVTAWPVEDVVALLKTGVSLQAGVSGPMA
                     EVVFRSLQYLSDADLRAMAVYLRSLPPQDKPEPPVAKPAADLMERGQDIYRQQCAECH
                     GEQGEGRRGAFPALAGNRAVTLADTTNLVRVLLQGGYQPATAGNPRPHGMPPFAQSLR
                     DEEMAAVLSYIRNAWGNVAPRVDTIDVYRARERRGS"
     gene            complement(19080..19715)
                     /locus_tag="PK518_00785"
     CDS             complement(19080..19715)
                     /locus_tag="PK518_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM47475.1"
                     /translation="MVARVLACTACHGKEGRATPSGYFPRIAGKPAGYLYHQLINFRD
                     GRRSYPQMSYLLEHLTDDYLREMAAYFAALDLPYAPPAATSAARALLERGQQLVRHGD
                     ASQKIPACAQCHGDALTGVAPFVPGLLGLSRDYVSSQLGAWQAGQRRAQSPDCMARIA
                     RQMAPGDVHAVATWLAAQVVPEQGKPAAEFKGKLPIPCGGVAAPAEAGSAR"
     gene            complement(19806..20561)
                     /locus_tag="PK518_00790"
     CDS             complement(19806..20561)
                     /locus_tag="PK518_00790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM47476.1"
                     /translation="MTQTVRIAIVTGAGTGIGKAAALALLRDGWSVALAGRREEPLLQ
                     VALESGAGTRALAVPTDVADPEAVRQLFDRTVQQFGRVDLLFNNAGVGAPAIPLEDLS
                     VQQWKDVVDINLSGMFYCMQNAFRVMKSQSPRGGRIINNGSISAHTPRPNSVAYTATK
                     HAVMGMTKTASLDGRKYDIAVGQIDVGNAETELAQRMTQGVQQANGQIAAEPLMDVAI
                     VGQSVLYMANLPLEANVMFHTVMATKMPFAGRG"
     gene            complement(20585..22912)
                     /gene="rnr"
                     /locus_tag="PK518_00795"
     CDS             complement(20585..22912)
                     /gene="rnr"
                     /locus_tag="PK518_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRM47477.1"
                     /translation="MYSKKKYSGPSAAQISSDEIEGSVQGHRDGHGFVIRDDGEPDIY
                     IPPNEMRAVLHKDRVRVRIVRQDKRGRPEGRVLEIIERPPQPIIGRLLQESGVWLVAP
                     EDKRYGQDVLIPKGATGSAATGQVVVVQLTEPPALFGQPVGRVVEVLGEVDDPGMEVE
                     IAVRKYGVPHEFSEACLAQAKALPDTVRALDKRRRVDLTDIPLVTIDGEDARDFDDAV
                     YCEPVKVGRGKGWRLLVAIADVSHYVETGSDIDIDAYDRATSVYFPRRVIPMLPEKLS
                     NGLCSLNPHVERLCMVCDMMVSAKGEVQAYQFYPAVMLSHARLTYNEVSAVLGNTRGP
                     EAAKYQDRVGNLLNLHDCYRALLTARQARGAVDFETTETQIICDDNGRIDKIVPRVRT
                     EAHRLIEEAMLAANVCSADFVEQAHRPALYRVHEGPTPEKQDILRGYLKAMGVGMSIS
                     DDPRPEEFQAIAEATKDRPDAQQIHTMLLRSMQQAIYTPINSGHFGLAFEAYTHFTSP
                     IRRYPDLLVHRVIKSILAGTKYALPALPTPGEAQFKLAKRLASKVAEPAIRPGKGAIS
                     VRDTQAWEAAGLHCSANERRADEASRDVEAWLKCQYMREHLGEEYSGVVSAVTSFGIF
                     VTLDALYVEGLVHITELGGEYFKFDEARQELRGERTGIRYSIGARVRVQVSRVDLDGR
                     KIDFRLVDETGETVQGKPAKARDGGKRPPEQDARLRDASQPQGFARSHDMRGHKDKDH
                     GHGGSGKKHGAGKSESRNGGKAGGKPGAKGRKPRP"
     gene            22932..23016
                     /locus_tag="PK518_00800"
     tRNA            22932..23016
                     /locus_tag="PK518_00800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22966..22968,aa:Leu,seq:cag)
     gene            23204..23288
                     /locus_tag="PK518_00805"
     tRNA            23204..23288
                     /locus_tag="PK518_00805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23238..23240,aa:Leu,seq:cag)
     gene            complement(23394..26021)
                     /locus_tag="PK518_00810"
     CDS             complement(23394..26021)
                     /locus_tag="PK518_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRM47478.1"
                     /translation="MSDAPEATPVQTKTTEPHPLDALTGGAFSAATSGERSARIREWL
                     VTQPATDQLQEVFKELSARDKGAARAVRERLDEIRRAQGQAVIAAEWQAKAEALLAAA
                     QLNIADALAWQRDAAKAGAALSREPLSLLKAQLSERVKTIEDLQHRVQVQREAAVLLA
                     QRIEVLSTKPWQDAQAALEALSADVGRWQEQAQVLTGDAAWPSVEARFPTQLESSRSQ
                     LLVVWEAFQSALALTVAAAQDAAAPLPPVPVWADELRQARGLPAEAAASAPARTAAKP
                     KVDPEVRDKAVQAVSEALAKLEQETAQGHGKASAGAAAALRAILKPHGRFIDAELERK
                     VHAALVAAGELEGWQRWSADQVREDLVARAEALLRRPDGQALGGRKMQETLRQLREQW
                     KQADQGAPANHGLWKKFDDACNAAHKVVESWLEKVRAEGVEHRAQRMALIEEVTTWAQ
                     EQGAAAAADWKAVNRALHQFGERWRAGGHVSEKVFAELQPLWKQALGAAAAPLETAQK
                     ASLQLRQAMIAEAEALGAAASLRIDAIKALQQRWQAEAQSVPLDRKHEQRLWDAFRKP
                     IDDAFNRKTVERERSAEQLSARDRAVLDASKALEAASASGDAQQIRAAMAALELAIKE
                     QAKAVESDANQPQTQADQAQTAINSEVNQESAKPVAAAPVRPVVAVRGDDRPGMRKAE
                     SAQPARAGRPGSERRDARDGRGPRDGAGRGREREAFEARGPRLGDTAFRAQRDAMEHA
                     QTALRKLAAQAHGEALTQLMAAWKSRDAEQLPTLQELGGKVSAGVRAAWGQAIAAAPA
                     GTGTEQALLRLEMAADVPTPADQLAARRALQLQMLTRRNDPAPAQTWAQDAAQVLAGA
                     SDEASARRLQSVLKVLLRK"
     gene            complement(26252..29950)
                     /locus_tag="PK518_00815"
     CDS             complement(26252..29950)
                     /locus_tag="PK518_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRM47479.1"
                     /translation="MAGLLGLWLLAWLAVPPIARAQIEKHASEALGRRVTVAKVEFAP
                     WSLEATVRGLSIASQDGQSPQVQIARIYIDAELQSLWRLAPVLDALQVDDPVIHITQT
                     APGEFDFDDVLAHLAQQRKPDEEPASPLRFALYNIALQGGAIDFNDRTVDKVHQLRQL
                     KLELPFLSNFAADREIRVIPRLAFDLNGSAFDSAAQATPFAQNRQTDLHLQIHGFDLA
                     PFSGYIPGSVPVRLRAGTLNADLKLDFEQVDATPQVKISGSAQLQGIQTLDVAGQPLL
                     SFDSLQVALNEVQPLRRIVDIAAIDWSGVHVHIGRDAKGALTLPGVNQPVDDASVKTP
                     PTAGVQESPWQVRLARLGVHDATVDWVDASLPGGTAVWKAENLQLQASAIELPFKQPL
                     QFRASARLSGGGAKGDPAWVALRGQATDAEAQAAASIRGLPLAMAAPYLAAVLKPALG
                     GMLDADIGLARNGAVMVAQVATLSLDNLVLTCAAEDKCPSLRRAGIADAGKTSVAELG
                     RLEVTDALVMLPEQRATVGRLLLHQPRMLLSRGPKGDWMFEQWLAQAPEQQPNHAVPK
                     KVEAPWALKLAALDVDGASIAFRDEVPERPVALNASGLQLHLRDVAFADGRLAPAAVR
                     LDTRVAAGRIEPGRLNWEGSVAMNPALSVQGKLRAQHLPLQAFEPYVTADLNVDILRA
                     DGSFTGSLRYVQDKAGPLVAVAGDASLDEVRVRAKDEDAGDGQDEGGMRIAQRGEELL
                     RWKSLALRGLAVNLKPGQPLALDVKETALSDFFARVIVHENGRINLQDIQKGAAAESR
                     VAAPSVQAASTPTAAPGEAAQTPAAQPADPMAPVIRFGPVALTNGSVRFSDFFIKPNY
                     SADLSELSGRLSAFSSVPAQAGGALQMADLELRGRAQGTASLEVSGKLNPLAKPLALD
                     IQGRMRDLELPPLTPYSIKYSGHGIERGKLSMDVSYKVQPNGQLTANNKLVLNQLAFG
                     DAVEGAPASLPVRLAVALLADRNGVIDLDLPISGSLNDPQFSLGGVILKVIGNLVMKA
                     VTAPFSLLAGMFGAADEQGVVHFVQGSSALDDKAHQLLDKIAEQLVNRPALKLTVVGW
                     AQPQAEQEAWKRLRLRDLAQAQKRRDAARAGESAADVAPVTDAEYPALLKEAYQRSDI
                     KKPRNLVGMAKDLPLPEMEALLLKSITVPDNAMQDLALARGVAVRDYLASRQVPLDRL
                     FVGAAKLQPAGEGGASWAPKAELALAAR"
     gene            30748..31569
                     /locus_tag="PK518_00820"
     CDS             30748..31569
                     /locus_tag="PK518_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRM47480.1"
                     /translation="MTASGNAIASARTFAADVINGFLEVTHNGFALLGLAVAFVAIAL
                     SARPDLRQAGEDELRGWLHERRVAEVGFPLEPTASERAVAINPKKLPREQAAVAFWLS
                     KKYRVAPEPLAALVTEAYEIGKRTKLDPTLILAIIAIESSFNPFAQSSVGAQGLMQVM
                     TSVHTDKYEGFGGQLAAFDPVANLRVGVKVLQECIARAGSLEGGLRYYVGAANLPHDG
                     GYAAKVLAEHFRLRQVAGRPAAATPVLSTKAPAATEIAPTAKPAEEKVALLDSDA"
     regulatory      31607..31772
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            31807..33051
                     /gene="glyA"
                     /locus_tag="PK518_00825"
     CDS             31807..33051
                     /gene="glyA"
                     /locus_tag="PK518_00825"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRM47481.1"
                     /translation="MYQRNILVEQTDPEVWAAIQAENLRQEEHIELIASENYASPAVM
                     AAQGSQLTNKYAEGYPGKRYYGGCENVDVIEQLAIDRIKQLFGAEAANVQPNSGSQAN
                     QAVLMAFLKPGDTILGMSLAEGGHLTHGMALNMSGKWFNVVSYGLNDKEEIDYDAFEA
                     KAREHKPKLIIGGASAYALRIDFERMARVAKEIGAIFWVDIAHYAGLVVAGEYPNPVP
                     FADVVTSTTHKSLRGPRGGIILMKAEHEKAINSAIFPGLQGGPLEHVIAAKAVAFKEA
                     LSPEFKTYQQQVAKNAKVFAETLTQRGLRIVSGRTESHVMLVDLRAKGITGKAAEAAL
                     GKAHITINKNAIPNDPEKPMVTSGIRVGTPAITTRGFKEEETRITANLLADVLENPND
                     EAHLAAVREKVNALTSRFPVYR"
     gene            33060..33509
                     /gene="nrdR"
                     /locus_tag="PK518_00830"
     CDS             33060..33509
                     /gene="nrdR"
                     /locus_tag="PK518_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRM47482.1"
                     /translation="MKCPFCSNQDTQVVETRVSEDGNFIRRRRQCGACEKRFTTYERP
                     DVHFPTVVKKDGRRVEYSRDKLLASFKLALRKRPVSTVQIDSAIERIEEKLLQLGQRE
                     VISSRIGELVMRELKKLDKVAYIRYASVYRSFEDIDEFRALVDEVRK"
     gene            complement(33573..34673)
                     /gene="aroC"
                     /locus_tag="PK518_00835"
     CDS             complement(33573..34673)
                     /gene="aroC"
                     /locus_tag="PK518_00835"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRM47483.1"
                     /translation="MSGNTLGTLFCVTNFGESHGPAIGCVIDGCPPGMELSEADIQQD
                     LDRRRPGTSRHVTQRNEPDAVEILSGVYQGKTTGTPIALLIRNQDQRSKDYGDIAQSF
                     RPGHADYAYWHKYGLRDPRGGGRSSARLTAPTVAAGAVAKKWLAEKYGTAFRACMTQL
                     GEQAIPFESWEHVPRNPFFAPVADVQAYEDYMDALRKSGDSCGARIRVQATGVPVGLG
                     EPLYDKLDADIAYVMMGLNAVKGVEIGAGFASVAQRGTVHGDSLTPQGFASNNAGGVL
                     GGISTGQDIEVQLAIKPTSSIISPRESIDIHGQSTEVITKGRHDPCVGIRAAPIAEAL
                     LALVLMDHALRHRAQCGDVRQAVPPIASAREV"
     gene            complement(34767..35222)
                     /locus_tag="PK518_00840"
     CDS             complement(34767..35222)
                     /locus_tag="PK518_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM47484.1"
                     /translation="MKVSDILRLKGNTLYTVHPDEPLARAVETMAEKDIGSLVVMEHG
                     DLVGMLTFREVIQRLVKNGGGVGTTLVRSSMDDAPVTCTLETDMDEVRRMMLGCHARY
                     MPVMDKRMLMGVVSFYDVAKAVVDSQNFENKLLKAYIRDWPQEESPPAA"
     gene            35331..36632
                     /locus_tag="PK518_00845"
     CDS             35331..36632
                     /locus_tag="PK518_00845"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HRM47485.1"
                     /translation="MPGYSDPGFDTLALHAGAQPDPATGARAVPIHLTASFVFESSDQ
                     AAALFNMERPGHVYSRISNPTNAVLEQRVAALEGGVGAIATASGQAALHLAIATLMGA
                     GGHIVASTALYGGSQNLLNYTLRRFGIETTFVQPGDLDGWRAAIRPNTRLLFGETVGN
                     PGLEVLDIPAVAQIAHDAGVPLLVDSTLTSPWLIKPFEHGADLVYHSATKFLSGHGTV
                     IGGVLVDGGSFDWEKSGRFPEMTQPYDGFHGMVFAEESTVGAFLLRARREGLRDFGAC
                     MSPHTAWLILQGIETLPLRMQQHMRNTEKLVQFLADQPFVARVGHPLLPSHPSHALAQ
                     KLLPRGAGSVFSFDIKGSRAQGKAFIEALKLFSHLANVGDCRSLVIHPASTTHHRLSN
                     EALAAAGIGQGTIRLSIGLEDADDLIDDLKRALKAAEKAGG"
     gene            36632..37447
                     /locus_tag="PK518_00850"
     CDS             36632..37447
                     /locus_tag="PK518_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM47486.1"
                     /translation="MYLQINGAQTFCHTGGKPFDAGQPSVVFIHGVLCDHSVWALQSR
                     HLAHHGWNVLAVDLPGHSRSGGEAPASVEQAADFIIALLDTLGVQRAALVGHSWGSLI
                     ALEATARLGTRASHLVLVGTAFPMKVSPALIESSLNEPEKALKMVNMFSRSSLASPSG
                     AGFWAYGAGLALGRRVLRSNPRVNVFHRGFVACDSYCGGEQAIAAIQCPVLFALGAQD
                     QMTPPKAAQGLIATARAAGKMVQVAHLPVGHNQMTETPEETLRAIGDFLNAPA"
     gene            complement(37452..38156)
                     /locus_tag="PK518_00855"
     CDS             complement(37452..38156)
                     /locus_tag="PK518_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM47487.1"
                     /translation="MVVTSLDTARIRAITIDLDDTLWPVWPTIARAEAALAGWLATHA
                     PATAALFGDAPALRAIRVRMNELRPDLRLDLSALRRESIRLALTQAGDDPALAEPAFD
                     LFFAERQRVELFDDALETLEFLSARYPVVALSNGNADVHHIGIGRYFKDSLSAARLGV
                     AKPDARIFHAAAQAAGVTSGAVLHVGDDALLDARGALDAGMQAAWLNPAQAAWPQGTP
                     PQATVTSLRELCRLLA"
     gene            38205..38594
                     /locus_tag="PK518_00860"
     CDS             38205..38594
                     /locus_tag="PK518_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47488.1"
                     /translation="MTQRNIFSTLRQPGMVLAMLALLAQLWMVQASARHWADMATQAL
                     FSADICSVHGQAGSPGPQGDEQPMGGMQHCPVCSVAGASFIASGGSTATLPRAPQGPP
                     PSHHDSQPQRESKRLRPPAQGPPQATA"
     gene            38690..40954
                     /locus_tag="PK518_00865"
     CDS             38690..40954
                     /locus_tag="PK518_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRM47489.1"
                     /translation="MISKTRILRGDGPLHGCALLPLAALLASLPLAATAQQDTALQTI
                     TVTATPVNETPVAVSEARQREIRAARPASSDSAQLLRNLPGVSLSGAGGVSSLPSVHG
                     LADDRLRIQVDGMDLISACGNHMNPPLSYIDPTRVGSVRLFAGITPVSVGGDSIGATI
                     QVESAPPTFAAPGEPARISGEAGAFWRSNGDALGAHLAASYAAEQFSLRYDGSTAQAD
                     NYKAARSFKSAGPAASDKPTQWLGDDEVGSSSYKTRNHALTMAWRGNTDLVELRLGLQ
                     DIPYQNYPNQRMDMTRNDSRQFNLRYQGQFGWGRLHARAYHENTRHAMNFGADKQFWY
                     GPAGNVPGMPMDTEGRTSGVQLRGDVELSERDTLRVGVLAQRYRLDDWWLPSGGGMAP
                     NTFWNIRDGQRDRLEAFAEWEARWSPQWLTQIGLRAGQVRMDSGAVQGYNSGYAAEAN
                     AFNARDRQRTDHNLDFTALARYTHNDQASYEFGFAHKTRSPNLYERYTWSTGGMAMRM
                     INLVGDGNGYVGNPDLRPEVANTLSATADWHDTTGERWGVKLTPYVTRVNDYIGAERC
                     TGGLGAMTSCTAANAGATQGFVYLRFANQDVRLHGLDLSGFSRLGDSAAWGRFTLHGM
                     LGYVRGKDRRTGDRLYAIMPLNARLALAHDQGGWSSMAELLMVGAKKHVSDLRNEVPT
                     AGYALLNLRTSYEWQAWRLDLGVDNVFNRFYQHPQGGAYLGQGATMAGTAVPWGVSVP
                     GMGRSFYLGATVKF"
     gene            41042..42082
                     /gene="hemH"
                     /locus_tag="PK518_00870"
     CDS             41042..42082
                     /gene="hemH"
                     /locus_tag="PK518_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRM47490.1"
                     /translation="MDLQKEPAPGPAHDTAVLLCNLGTPDAPTAPALRRYLAQFLSDP
                     RVVEIPRAAWLPILHGIILRTRPAQSAAKYASIWTDEGSPLAVWTHKQAVMLRGWLGE
                     AGIQAQVLPAMRYGQPAIAAQLQALQDAGVRRVLVLPLYPQYSATTTASVVDAVNAWT
                     RDARHFPELRFVNDYHDHPGYIAALAASVRAHWRREGARGDKLVLSFHGIPERNVRLG
                     DPYADQCRSTAHLLAQALGLADDEWLLTFQSRFGRARWLEPYTQPTLERLAAAGLASV
                     DVLCPGFAADCLETLEEINMEVREAFLHAGGKRFAYIPCLNDNREWITALSSIAQTHL
                     AGWTAPAAKVQP"
     gene            42079..44520
                     /locus_tag="PK518_00875"
     CDS             42079..44520
                     /locus_tag="PK518_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM47491.1"
                     /translation="MTPPCPRLAPEQLRLHIAPESLGFADTRALVGEPLPWIGQQRAF
                     EAARFGLGLQQPDYHLFVLGEVGSGRASLMRQAMKEAAAGRPVPPDLCFLHDFDATER
                     PRALRLPAGQGRQLRDQIAAWSRLLEAEIPKRLAAPDVKSESERIVKRYQSDEAQAFG
                     ELAEFARARRFRLVRDEGQMLFTLLGPGGEEPLIEAEAAALSAPQRSPLEQSEQELRA
                     EIVRFLDRTRPLERARDEALAQLLRQSAGPLVTHGLDDVRQGLRKQIKDTVKLGRWLD
                     QVEEQVLDNLDLFVPHEGGQGEDALEDERREQLADLLALCQVNLAVDNTGLAGAPVLV
                     EDNPQVRNLFGHIQYESGEDAPYADFSGIHAGGLLRAHGGFLMLFLRDLAGDAPLWER
                     LRRFLRCNRVSIEDQAAGHGSGAPVALQPEGVDVEVKIVLVGSAAEYYLLQDGDADLA
                     RRFRVKVDFAEQFRASDATHHASCVFLARSCARRGLPHCTAAAAARLLEFTHREADDQ
                     ARQSASFASLEALLVESCAQARARAAQLTDARDVDAALAARRTRHNAPEEALHEAIAE
                     GELLITLEGQRVGLLNALTQIDMGDYRFGFPVRITARTHAGREGVVNIEREVEMSGPI
                     HDKGVLILQSYLSALFGHLAPLALNASIVFEQEYSGIEGDSASCAELYALLSSLSGLA
                     LRQDIAVTGALNQHGEVLPVGGLNEKIEGWYRVCAQAGLTGTQGVLIPARNQRHLMLS
                     PQVVQAVADGQFHVHAAQHVSEGVALLTGHDAGLAPGECLPGSVHARAEATLLAYRRA
                     WQAMALGRRLPQRGR"
     gene            complement(44535..44747)
                     /locus_tag="PK518_00880"
     CDS             complement(44535..44747)
                     /locus_tag="PK518_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HRM47492.1"
                     /translation="MIFGYTEQQIAHFFLTYGVGAFILFMVFIILQLARESKAGRFGT
                     FVIFLGLGVGFIGYLAKIVIQWWMER"
     gene            complement(44778..45623)
                     /locus_tag="PK518_00885"
     CDS             complement(44778..45623)
                     /locus_tag="PK518_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRM47493.1"
                     /translation="MPHSLRNRPASPLVLPQVRTIGLLRPLAWLTLALRDIARAPLLS
                     LAHGLVLALLGAAILSLGYNRFWFVAGTLSGFLIVGPMLATSLYAISRALERGERVGL
                     GLVWQTWASWQRSHAGQAQHQGYWSLVRFGLLLSLAATGWVAISASLIYTLSPVRVDT
                     PMDFLRHVVLAREGALFAIWLALGSFLAAPIFASSVVAMPLLLDRRITVRQAVLTSWA
                     VVLANPVPMALWAALIVVLTLLGLGTLLIGLVVVMPLLGHASWHAYRELVDASAFAPL
                     DAPAP"
     gene            45804..46748
                     /locus_tag="PK518_00890"
     CDS             45804..46748
                     /locus_tag="PK518_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM47494.1"
                     /translation="MKKLLALAALATAFHTAWAAQPLLTPAELSAQQAQASAPRVIDI
                     RDPASYSAQHIPGAINAPYGKWRGPASNPGELLDAPKLTELAQSLGLTASTHAVIVST
                     GADATDFGAAARVYWTLKLLGLKELSILNGGMKAWADAKLAQDQAVPQVARSQYQPQI
                     DTSLLATREDVGQHIKLSNAALVDSRPDAFFLGKTRAPAAKLAGTLPGAQQLDFNQWF
                     LPGTATFVDKSAAQKIAAQVKREQGQDMVAFCNTGHWAATDWFGLSEVAGLPGVKLYA
                     GSMVDWTQSKDTPLMANQPSRFEALAYDAKQWWERKSK"
     gene            46947..48044
                     /locus_tag="PK518_00895"
     CDS             46947..48044
                     /locus_tag="PK518_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRM47495.1"
                     /translation="MKRLPLAAVLAVFTGWLLWSVSTRQAALFAVGLGLGAVLAGQRF
                     GFTTGWRMLIEQRDASGVMGQLLLLALAAALAMPLLGHYPELTAALGPPSVSLLVGAF
                     VFGLCMQIADGCGSGTLYKAGLGIPMNAAILPLFALGSFLGSLHLGFWLDLGRVQPVG
                     LVNQFGWVQALLITLAALALAAVAVRWYVGRASQAAGQAPKPLLVRKWMLGAVLLALL
                     ATANLLIAGQPWGVVYGFGLWAAKLAQATGAADLAGNWFWSQGGNAARLQETVLMDVT
                     SITNIGILGGALWVSAGKATTAKPLNGTQWAVALVAGLALGYSSRLAFGCNVGAMLSG
                     ISTGSIHGWIWVPLAFAGTIFGLRIRRHFGF"
     gene            48062..48229
                     /locus_tag="PK518_00900"
     CDS             48062..48229
                     /locus_tag="PK518_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47496.1"
                     /translation="MTQHTTLRLVFWAILVAFLAWDWAGSKPVDLRLEPPLIAAGSGQ
                     AATGGHCSMPD"
     assembly_gap    48429..48528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<48529..50622)
                     /locus_tag="PK518_00905"
     CDS             complement(<48529..50622)
                     /locus_tag="PK518_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HRM47497.1"
                     /translation="MTALSAATLAACGGSGPSNELPKGIKEISATAYPATTVGKGDTA
                     ATQDLLTGGMGRTGLGAAAAPAYADPANPTAAELRRNALYSNYRGILDPSAGGGYGLL
                     YGPNVTVDGQVTSNEGLIPGREYVAVLDDGTGRKQTVIAVQVPDSFSASNPCIVLGAS
                     SGSRGVYGAIGTAGEWGLKKGCAVALTDAGKGVGLHNLSDDTVNRIDGTRATRTVAGA
                     LSFFAANLSDAARAAYNAVLPNRIAIKHAHSQQNPEKDWGLDTLAAGRYALYVLNARY
                     GTPDNRVPFTAGNTLVIAGSASNGGAASLRAAEQDGDGLIDGVVASEPVTEMPTTAGY
                     GIQFGGAAVAGYGKTLADYTTYGNIYQPCAALAADAAMAETSIYNYIALTAMTARATA
                     RCDGLAAKGLVTGATTAERAADALARLRAYGWSSDNDSMHNAHYALGNGPILSAMYTM
                     AYGRFGVDANLCGASFAAANATGDVVAAAPAALASSFATANGTANGTPATVIYNDSKG
                     GAKSWPWAVSPSTGVADFGLDNALCQHALVSGKDLATGAALTASSQPSKAQSDAVRAG
                     IAEVLHSANLRGKPTIIVAGRSDALVPVNHNARAYTALNRTIEGAASKLRYIEVVNGQ
                     HFDAFLPFSGFDTRFVPLHPYFNQAMDVMWAHLKSGSALPASQVVRTTPRGGTAGAAA
                     APAITAANVPPFAMAP"
     gene            complement(50781..52310)
                     /locus_tag="PK518_00910"
     CDS             complement(50781..52310)
                     /locus_tag="PK518_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRM47498.1"
                     /translation="MEIFDALMAGFATAITPANLLWALVGCALGTAVGVLPGIGPAVA
                     VAMLLPITAKVEMTASMIFFAGIYYGAMYGGSTTSILLNTPGETASMVTAMEGNKMAK
                     SGRAGAALATAAIGSFFAGTVATVVVTLFAPSVAEFAVKLGPPEYFMLMVLAFTTVSA
                     VLGQSSLRGMTALFIGLALGCVGMDQISGAARYTGGKMELLDGIDIVLVAVGLFAVAE
                     VLYAALFEGKVEESQNKLTRVHMTARDWKRSWPAWLRGTVIGTPFGCIPAGGTEIPTF
                     LSYATEKKLARGADKAEFGTQGAIEGVAGPEAANNATVTAALIPLLTLGIPTSNTTAV
                     LLGAFQNYGLNPGPQLFSSSAALVWALIASLYIGNLMLLVLNLPMVGLWVKLLKIPRP
                     QLYAGILIFATVGAYGMRQSTFDLFLLYAIGLLGVLMRRFDFPTAPVVVGMILGPLAE
                     AQMRNAVSIGEGSWWIFLQRPMSLTLVIIVLAVLILPRLLRRWAERNLARARQNVDDV
                     S"
     gene            complement(52310..52867)
                     /locus_tag="PK518_00915"
     CDS             complement(52310..52867)
                     /locus_tag="PK518_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRM47499.1"
                     /translation="MVEPVSSDLLAPPTEAGSIPSPRWQAAVGAAVVLVAAGMALGAL
                     QIPGDAGYGGVGPNFLPWLCAAVLALCGVLLVREALTGGYRQAADGGGEPRAALAPFA
                     WVTAGLLLNAALIETLGFILGCTLCYALAVQGLRRANGQQGLLAPRALAVDVFTGLAI
                     AAPVFWLFTQFLAINLPGLTNTGWL"
     gene            complement(53007..53969)
                     /locus_tag="PK518_00920"
     CDS             complement(53007..53969)
                     /locus_tag="PK518_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRM47500.1"
                     /translation="MRRDTFLKSLAALAAVGSLPLPARAAVAMKMMLPANPGGGWDTT
                     GRALGKALQDAGVASSVTYENKGGAAGAIGLAQFINGSKGDPHALMVMGAVMVGGIIT
                     GKPPVNLGQATPIARLTSEYNVFVLPANSPFKTMAEVVAQLKKDPGSVKWGGGSRGAT
                     EHIAAAMIAREVGVDPAKINYVAFRGGGEATAAILGGNVTIGGSGYSEFAEYINAGKM
                     RAIGVTSPKRLKDLPNVPTLKEQGINVEIGNWRGVYGAPGINAEQRKALIAMVDKAVK
                     SKAWAEALEKNGWTPAWLAGEEFGNFVDSEFASLRATMVKSGMV"
     gene            54111..54794
                     /locus_tag="PK518_00925"
     CDS             54111..54794
                     /locus_tag="PK518_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM47501.1"
                     /translation="MQLLLVEDDATMQATLQRSLSRRGMAVTALADGRAALAEWRAHP
                     PDAVVLDLTLPGLDGLQVLAQARAAGLRTPVLLLTARGTVGDRVLGLNAGADDYLPKP
                     FDLDELEARLRALLRRSADGSQTPTPPEGPVVGALRYDKDSGVIYLQDAPMDLTPREQ
                     ALLRALLAQPGRALPKERLYELVFPGQSEVQYEAIEVVVYRLRKKLTGTGATLMTLRG
                     LGYLLKTET"
     gene            54791..56227
                     /locus_tag="PK518_00930"
     CDS             54791..56227
                     /locus_tag="PK518_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HRM47502.1"
                     /translation="MTRQRSLRRQLLLGILLPTLLLIGVNTYSLYHQALAALNTAYDR
                     TLLASAKSISEQLDVRGYDDAAELRATVPYSALEAFEADNQSRMFYRVSTLQGGLVSG
                     FAELPFWQGSIAQRPPYAALVDFYNARFRDRPVRVAALLQPVAGPQGRTMAVIQVAET
                     LELRHAAALQILWNTLVRQALLLAMIAAIVVLVVQRATLPVRRLSSELQGRGADDLSP
                     IAAPAAPRELQPLIDATNAVMQRLSRLLAHQKRFVRDASHQLRTPLAVLKTQVQSALR
                     GDLPPTQALQEIGDTVERATQLANQMLALAKVEQLRQQGEPPVTRLDEVLREVALDLS
                     PLIAQANLDFGISTDTAPIQAHAWMLRELCRNLLHNAIRHAPPTTELTVALHTQGAQA
                     LLTIADAGPGVDDELAARLFEPFSAGDMRHSSGLGLAICQEIVQALGGNIALGNRRQG
                     GAILGLDAVVRLPLARPREAAPVKKSRP"
     gene            56224..56649
                     /locus_tag="PK518_00935"
     CDS             56224..56649
                     /locus_tag="PK518_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRM47503.1"
                     /translation="MTTPPDSMRLDKWLWCARFYKTRSLAVEEIGKGRVTVNGQAAKA
                     ARELRVGDNVALRQGPVARTVVIRALSNMRGPAPVAQQLYEETPESIAARAQAAEARR
                     LAPEPATALREGRPTKRDRRDMDDLRHGWGERWSASLDD"
     gene            56730..57188
                     /locus_tag="PK518_00940"
     CDS             56730..57188
                     /locus_tag="PK518_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRM47504.1"
                     /translation="MIYNALKLAHVLSIIVWIGGMVFAHFFLRPAAQALEPAQRVTLM
                     HGVLQRFLGAVAIAIVVVLTSGLGMIGAVSMGSGGAFSMPLKWTIMATLGLVMMAIFA
                     YIYFALLDRLEQAVTASDWSAGGLALARIRSWVGVNLVIGVVIVVQTLLM"
     gene            complement(57289..57516)
                     /locus_tag="PK518_00945"
     CDS             complement(57289..57516)
                     /locus_tag="PK518_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRM47505.1"
                     /translation="MDIHKEIDTRGLNCPLPILKAKKALADMESGQLLRVVATDSGSV
                     RDFQAFARQTGNELVEQQTVGTEFIHVLRRR"
     gene            complement(57522..58088)
                     /locus_tag="PK518_00950"
     CDS             complement(57522..58088)
                     /locus_tag="PK518_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM47506.1"
                     /translation="MQYQTRFCSHCATALAPIVTMEDSGEKERLRCPACGWTHWNNPT
                     PVLAAIVEVDGRILLARNALWPAKMFALITGFMEAGESPEEGIAREVKEETNLDTRAV
                     RLVGAYEFLRMNQVIIAYHVQAQGQVRLSPELVDYRFYELDELKCWPAGTGYALADWL
                     RSRGHEPVFFTAEENAERRRGLDEAPKD"
     gene            complement(58103..59008)
                     /gene="cysM"
                     /locus_tag="PK518_00955"
     CDS             complement(58103..59008)
                     /gene="cysM"
                     /locus_tag="PK518_00955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HRM47507.1"
                     /translation="MKTYPTIEDAIGRTPLVALQRIAAQDNAARGNVILGKLEGNNPA
                     GSVKDRPALAMIRRAEERGEIKPGDTLIEATSGNTGIALAMAAAIRGYRMVLIMPEDL
                     SVERAQTMKAFGAELILTPKSGGMEYARDLADQMVVQGKGVVLDQFANADNPRIHYET
                     TGPELWEQTEGRITHFVSAMGTTGTITGVARFLREKNPGVRIIGAQPQEGSRIPGIRK
                     WPEQYLPKIYQPNLVDELVLVSQDDAEDMARRLAREEGIFGGISAAGACWVAQQIAAQ
                     EQNATIVFVVCDRGDRYLSTGVFPA"
BASE COUNT         9883 a        20349 c        18879 g         9880 t
ORIGIN      
        1 gatttcactc ccactcaatc gtcgccggcg gcttgctgct cacgtcgtag gtcacgcgat
       61 tgatgccgcg cacttcgttg atgatgcggc cggacaccct cttcaaaaga ctgtagggca
      121 gctcggccca gtctgcggtc atgaagtcgc tggtctgcac ggcgcgcagc gccaccacgt
      181 agtcgtaggt gcggccgtcg cccatgacgc cgacgctttt taccggcagg aaaacggtga
      241 aggcctggct cgtcaggtcg taccaggtct tgccggtggc ttcgtcggtg aagttgcgca
      301 gctcttcgat gaagatggcg tcggcgcggc gcagcaggtc ggcgtagtct ttcttcacct
      361 cgcccagaat gcgcacgccc aggccggggc cggggaaggg gtggcgatag accatctcgc
      421 gcggcaggcc cagggccacg cccagctcgc gcacctcgtc cttgaacagg tcgcgcagcg
      481 gctccagcag cttcaagccc aactgctcgg gcaggccgcc gacgttgtgg tggctcttga
      541 tggtgacggc ttttttgctc ttggcgccgc ccgactcgat cacgtcgggg tagatggtgc
      601 cctgggccag gaaggtggcg cccttgtggc cggcattggc ggctttcagc ttggccgcct
      661 cggccttgaa cacgtccacg aacaggccgc caatgatctt gcgcttttgc tcgggttcgc
      721 tcacgccggc gagcttgccc aggaacaggt cgctggcgtc cacacggatc accttggcgt
      781 gcaacttgcc ctggaacatg tccatgacca tgtcgccttc atgaaggcgc agcaggccgt
      841 ggtcaacgaa cacgcaggtc agctggtcgc cgatggcgcg gtggatgagc gctgcggcca
      901 cactggaatc gacgccgccg gacaggccaa gaatcacttc ctcgtcgccc acctgctcgc
      961 ggatcttggc aaccgcttct tcgatgtgat ccttcatcac ccagtcggcc tgcgcgccgc
     1021 agatgtcccg cacgaagcgc gagagcagcg ccatgccctg cacggtgtgc gtgacctcgg
     1081 ggtggaactg cacggcgtaa tagccccgcg cctcgtcggc catgccggcg atggggcagc
     1141 tgggcgtgct ggccatgagc ttgaagcccg gcggcagcgc cgtgaccttg tcgccgtggc
     1201 tcatccacac cttgagcatg ccgtggcctt cggcggtggc gaagtcctcg atgcctttga
     1261 gcagcgcggt gtggccgtga gcgcgcacct cggcgtagcc aaactcgcgg ctgttgctgc
     1321 cttcaacctt gccgcccagc tggttggcca tggtctgcat gccgtagcag atgcccagca
     1381 ccgggatgcc caactcgaac acggcgtccg gcgccttgtc gtccacttcg tagacgctgg
     1441 cgtgactgcc cgacaggatc acgcccttga gcctgccgtc ggcggcaaat tcgcgcaccc
     1501 agtcgctgct cacgtcgcag gggtgcacct cgcagtacac atgcgcctcg cgcacgcggc
     1561 gcgcgatgag ctgggtgacc tgggagccga agtcgaggat gaggatcttg tcgtgtgcca
     1621 tggtggttta taaagaaatc agcctctagc gcttgccagt caagcgttag aggctctcaa
     1681 aataatagtg attggtggct tagtcggcgc ggtagttggg cgcttccttg gtgatttgca
     1741 cgtcgtgcac atgcgattca cggatgccgg cggcggtgat ctcgacgaac tcggccttgt
     1801 cgttcatctc ggcgatcgtg gcgcagccgc agtagcccat ggcggcgcgc acgccaccag
     1861 ccatctggaa gacgatgctg accatgctgc ccttgtaggg cacgcggcct tcgatgccct
     1921 cgggcaccag cttgtcggcg ttggggttgc cggtggtcgc ttcctggaag tagcgatccg
     1981 cgctgccctg ctgcatggcg ccgatggagc ccatgccgcg gtagctcttg tagctgcggc
     2041 cctggtagag gatgacctcg cccggcgctt cctcggtgcc ggcaaacatg ccgcccatca
     2101 tgatggtcga cgcgcctgcg gcgatggcct tggcgatgtc gcccgagaag cggataccgc
     2161 cgtcggcaat caagggcacg cccgtgccct tgagcgcctg ggccacgctg tcgatggcca
     2221 tgatctgcgg cacgcccaca ccggcgacga tgcgcgtggt gcagatcgag cccgggccga
     2281 tgccgacctt gacggcgtcg gcaccggctt cgaccagcgc cagcgcagcc gcgccggtgg
     2341 cgatgttgcc gccgatcacg tccacctgcg ggtagttctg cttgacccag cgcacgcgat
     2401 cgatcacgcc cttgctgtgg ccgtgcgcgg tgtccacgac gatggcatcg accccggcct
     2461 tgaccagggc ggcgacgcgt tcctcggtgc ccgcacccac acccacggcg gcggccacgc
     2521 gcaggcggcc ctcgctgtcg cgcgcggcgt tggggaaggt ggtctgcttg ttgatgtcct
     2581 tgacggtgat caggcccttc aattcaaagg cgtcgttcac caccaagaga cgctccagct
     2641 tgtgcttgtt taaaagtgcc ttggcctggg ccgggctggt gtggtcacgc tcgttgaccg
     2701 tgatcagctt ctcgcgcggc gtcatgatct ggctgacctt gacgtcgtaa cgcgtctcaa
     2761 agcgcacgtc gcggctggtg acgatgccaa tcaccttgcc gccgtcgcac accggaaaac
     2821 ccgagatgcc caggttttcc gacagctcca gcacctgaag caccgtgtgc tcgggggtga
     2881 tgaccaccgg gtcatgcacc acgccagact cgtggcgctt gaccttggag acctcggcgg
     2941 cctgctgctc ggccgtcatg ttcttgtgga tgacgccgat accgccctct tgcgcgatgg
     3001 caatcgccag gcgcgcctcg gtcacggtgt ccatggcggc ggacacgagt ggcaggttca
     3061 gggtgatgtt gcgcgtgaag cgcgtggcga gggaggtgtc cttgggcagg acctgggagt
     3121 acgctggcac caacaacaca tcgtcgaagg tgagcgcttt tccgagaagg cgcatgtgaa
     3181 agctccaaaa aaacgattgt acccgtgact gccgctgtcg aaaaaacgac atgccgtggc
     3241 cttgttggcc gccagaaagg tctcgccgtt acactcgctt gcatgaaacc gcacaagctt
     3301 cttctgctgg cattggcatg cacctgggcc acgggcgcgt tcgcgcaatg gcagtggctg
     3361 gacaaggacg ggcgcaaggt gttcagcgac cggccaccac cgtccgaggt gccggagaag
     3421 aacatcctca agcagcccca catcagcagc cggcctcgcc ctgcggcccc gcccgtaggc
     3481 gcagaggcca acgcggatac gcccgtcacg gcccaggcca cccccggcca aggcaaggac
     3541 aagcagctgg aagaaaacaa ggccaaggcc gaggccgccg aggccgccaa gaaaaaggcc
     3601 gaacaagagc gcgtggccaa ggcccgcgcc gacaattgcg cacgcgccag gctcaacaag
     3661 gttcagctgg cccctggtcg attggtggcc cataccaatg ccaagggcga gcgcagctac
     3721 atggacgatg ccacgcgcga ggccgagctg cgccgggccg acgacatcat cgccgcggac
     3781 tgccagtaac gccgcctggt gccggccatc ggcacctcaa taacccgcct tggagccagg
     3841 ccggcgcttg gcaaacaggc ccgcaacacg cgcgccctgg gccttgaagc gctggcgcct
     3901 tgccagtttc gggtccaccg tcagcggacg gcagcattcc aaccggtcgc catcacgcaa
     3961 cacctcgtgc gctttggctg gcctgcccca gatgccgcag gccagccgat cggcctgcgg
     4021 catgagcccg caggcccgca gcgcgtcagc cacggtggcg ccctgcgcca attgcagctg
     4081 ccactcctgc acctcgccgg gccgcagcga tgcggccacg gtgatacgca ggcttgccgc
     4141 cccgccctca gccatagaca tcctcggcgc gcttgatgaa ggcgtccacg aaggtggcgg
     4201 caatgcggtc gaaaaccggg ccgaccaggg ctgccagcgt gcggctgtca aagccgtaat
     4261 gcaattgcag ctccaccttg caggcacgct gcgtgccgtc gcccacgggg tggaaatgcc
     4321 aatcgccatc aagcttggag aacgggcctt cgaccagctc catcttcacg cgccggccct
     4381 gctcgtgggt attgcgcgtg acgaaggact tgcgcaggcc gcccagggag atgccgacct
     4441 cggccttcat tccatgggcg tcttgctcca ggacagtgct gtggtcgcac cagggcaaga
     4501 actccgggta gctggcaacg tccgtcacca gggcgaacat ttcttcgggg ctgtaccaga
     4561 tgaggacgga tttgttgact gttttcatgg aggcgggcgc ctagaatggg gggcgcgaga
     4621 cgacaacgat tgtagggagg ccttggaact cccggtttgc gccgcacctg aataacatgg
     4681 ccaagaaacc cgatacatcg tcacgcatcg ccgacaacaa gaaggcggca tacaactact
     4741 tcttcgagga acgccacgag gccggcatgg ttctgcatgg ctgggaggtc aaggcgctgc
     4801 gcgagggcaa ggtgcagctg accgatggct acgtggtcat caaggacggc gagctgtttt
     4861 tgatcggctg ccagatcaac cccctgaaaa ccgcctccac ccacgtcaac cccgacgcca
     4921 cgcgcatcaa gaagctgctg ctgcacaagg aagagatccg ccgcctgatt ggcaaggtgg
     4981 agcaaaaggg ctacaccctg gtgccgctga acctgcactg gaaggacggc cgcgccaagt
     5041 gcgacatcgc cctggccaag ggcaaggccg agcacgacaa gcgcgacacc atcaaggatc
     5101 gcgaaggcaa gcgcgaggtc gaacgcgcca tgaagagccg ccaccgctga ccggtttttt
     5161 cgacagggcg tggaataaat gcccctgcca tggtgttcat ccctgcgtcc ggccgcaatc
     5221 tcacccggtc ggcaccacga cggagaacct catgaccaaa ctcaactccc tgctcgccgt
     5281 gctggccctg gccggctgct attccatgcc ccatgcccag gtgatgaccc agggcggcgc
     5341 cctggtcggc gccaacggca tgacgctcta caccttcgac aaggacatgg ccggcagcgg
     5401 caagtcggtc tgcaacggcg cctgtgccac caactggcca ccgctcaccg ccacggcggc
     5461 gcccggcggc gagggctaca gcctggtcac gcgcgacgat ggcaccaagc aggtggccta
     5521 caagggcaag ccgctgtact actggagcaa ggacatgaag ccaggcgaca agaccggcga
     5581 cggcgtgaac aacctctggc gcaccgccac accgtaagcg cccatgaacc gcgcccaggt
     5641 cgtggagcaa ctgcccggac tgcgccgcta cgcgcgtgcg ctcaccggca acgcctgggc
     5701 ggccgacgat ctggtgcagg acacgctgga gcgcgcctgc cgcaaatggc tgctctggcg
     5761 cagcggcagc gatctgcgcg cctggctgtt cacgctgatg cacaacctct acctgaacca
     5821 gcgccgcggc ctgctggagg tgcaaccgct ggacgatcag gccctggcga cactggcggg
     5881 cacggatgat gcagcggccg acatggccat ggatctggat cgctgcctgc agcgcctgcc
     5941 cgccgagcag cgcgccgtgc tgctgctggt gacgctggag gacatggcct atgccgacgt
     6001 ggcgcgcgtg ctggacattc ctgtgggcac tgtgatgtcg cgcctgtcgc gcgcccgcag
     6061 ccgcatgcgc gagctgatgg agaccgcgcc tgcccaggca ccggccgccg gcgcagcccc
     6121 gccagccccg ctgcgccgca tgaagtgaca cccatcgcga cgatgccgat catgaacacc
     6181 tcttccagcc cccaggatcc ggacgcatcc aggctgcacg ccctggtgga tggccgcctg
     6241 ccgcccgaac aggccgcgct tctgcgcgcc agcctggatg ccgctacagg cgaggatgcc
     6301 gacgcctggg cgcgccagcg cgcgctgctg cggaccctgc acggcgactg gctgcagcgc
     6361 cccctgcccg aggcgctgca ccaggcggcc gagcgcctgc agaaccagca tgcccagcgc
     6421 cggcgctggg ccacctggag cggcgtggcc gcgggctggg tactggcctt cggcctgggc
     6481 tggggcctgc atggggcaag cggcggcggc gccacgcagg ccatggcggc gccccggctg
     6541 tttgcccagc aggccaccgt ggcccatgcc gtctaccagc ccgagcagcg ccaccccgtc
     6601 gaggtgacgg ccgcgcagca ggagcatctg gtgcagtggc tgtccaggcg cctggagcgc
     6661 ccgctgacgg tgccgcatct gcaggatcag ggctatgagc tggtgggcgg gcgcctgctg
     6721 cccggcggca gtggcgcgcg cgcccagttc atgtaccaga acgccgccgg ccagcgcatc
     6781 accctgtacc tgggggcgct ggagcaggcc caggccgagg ccttccactt ctacggcgac
     6841 ggggcggtat cgagctttta ctgggtcgat cgcggctttg gctacgcgct cagcggcgcg
     6901 ctgccgcgcc cggcgctgca ggcgctggcc acggccgtgt accagcagct cagcgcagcg
     6961 ccgccagcgg gtgcgcgtgc gccggccagg ggctcttgaa gaactgctgc accagctcag
     7021 gcgccacgtc ctcgatgcgc gccgggttcc aacgcgggct ctggtccttg tccacggcca
     7081 gggcgcgaat gccctccagg gtttcactct ggcccgggcg caggtagaag cagtggcgca
     7141 ccatatcgcg ctccatgcgc aggtcgtcgg ccaggtccat ggtgcgcgcg cgccggatct
     7201 gctccagcac cacatgcagc atcagcggcg agcgcttgcg cagcaacgct gccgtggcgc
     7261 gcgcccagtc gtcgccggcg ccctccagtg cggcgacgat ggtgggcaca tcaggcaatg
     7321 agaagtattg gtcaatttgg cctctagcgc ttacttggca agcgccagaa gctatcattt
     7381 ttgactcaac ccactgctgc acggccgcgc cgctggcaaa gctttgcgcg cccagggcgt
     7441 cccacagccc ggcctgctgg tcagcgggca agaagccgtc ggccagcttg aaggcgatgg
     7501 cgtcggccgc gccaatggtc tcgccgctca aggccagcca ctcgcccacc cggcccgggc
     7561 aacgcgagag gaagtagccg ccgcccacgt cggggaacag gccaatgatg gtctcgggca
     7621 tggccatctt ggtacgctcg gtcacgacgc gcaggctggc cccctggctg atgcccatgc
     7681 cgccgcccat gacgatgcca tccatgaagg cgatgtaggg cttgccgaag ttgtggatca
     7741 ggtggttgag ggcgtattcc tcggtgaaga agtcctccaa ctgcgggttg cccacggtgc
     7801 cggcctggtg cagaaagcgg atgtcgccac cggcgcagaa ggcgccgaag gcaccttcct
     7861 tgttgctgcc gcgaatggcc acggccagca cctggtcgtt cttctgccag tccagcagca
     7921 cggccatcag ctcgcgcacc atgcccagcg acagggcatt cagcgcccgg gggcggttga
     7981 gcgtaatgca gcccacctgg ccgcgcagct cggcaatcac ttcatgtgtt gtctcggtca
     8041 tggttgttcc ttccatccca gcagttcatt gaaaaccagc gcgccgatca ccagcgtgcc
     8101 gcccatcagc acggccgggg ccgggcgctc gccggcgccc acccagacca gcacgatgcc
     8161 aaaaatcact tccagcaggc ccagcagcgc gatctccggc gccttgagca cacgcccgca
     8221 gtacaccacc agcacgcagg ggatggccag ctgcaccagg cccagcagcc caagcaggga
     8281 cagatcacgc gccgatgcct gaaacggcag ggcccagggt agcgtggccg cggccgacag
     8341 cgccgcgccg atcagcaccg ccggcaccag atcgaccgcc tggccatggc gctgcgaatg
     8401 ctgcaccacg gtccagttga tggccgccgc cacgggaatg cacaacgcca cggccagccc
     8461 cacgggcgac agggagcgca cctcggcgcc atacatccag acgatgccca ggcccgccac
     8521 cacgatggcc gccgccgtgc gcggtgctat gcgatggcct atgaaaacgc gcgccaccag
     8581 ggccgtgagc atgggcccga ccgccatggt gaccagcaca ttggccaccg gcatcagcgt
     8641 gatggccacc atgaaggcgg caaacatcac gctccagcac aggcccgaca gccacagcgc
     8701 gcgcggcccg cgccatacct tggcaaacac gtcgcgcccg ctcagccagg gcaggatgac
     8761 gagcaacgag agcagcatga agaagctgcg ccagaaggtc acctcgaagc tgcgcgcctg
     8821 ctccaggtgg cgcgtgacca cgccggcgat agaccacatc agcgccgcca gcgccatcag
     8881 ccatacggcc cggccatgcg tcaggccagg gcgcgcgttg gcgggatgtg cctcaggcgc
     8941 cttcacggct ggcgcgcttg cgctcgtgtt ccttgaggtg gcgcttgcgc aggcggatgc
     9001 tcttgggcgt gatctccacc agctcgtcgt cctcgatgaa ctccacgccg tactccagcg
     9061 tcaggtcgat cggtggcgtg atcttgatcg cgtcttcctt gccgctgacg cggaagttgg
     9121 tcagctgctt ggtgcgcgtg gcgttcacca ccaggtcgtt gtcgcggttg tggataccca
     9181 cgatcatgcc ttcgtacacg gggtcgttgg ccttcacgaa catgcggcca cggtcgtcga
     9241 gcttgcccag ggcgtaggtg aagatttcac cgtcgtccat ggagatcagc acgccgttct
     9301 tgcgaccgcc gatctcgccc ttgtgcggct cgtagctatc aaaaatattg ctgatcaggc
     9361 ccgagccacg cgtcaggttc aagaattcgt tggtaaagcc gatcaggcca cgcgccggga
     9421 tgcggtactc caggcgcacg cggccacggc cgtccggctc catgttgacc agctcgccct
     9481 tgcgctcgcc cagcgcctgc atcacgccgc cctggtggcc ttcttcgatg tcggcggtca
     9541 ccagctcgat gggctcgcag cgctcgccgt cgatgtcctt gaacaccacg cgcggcttgg
     9601 agacagccag ctcgtagccc tcgcggcgca tttcttccaa gagaatggtc aggtgcagct
     9661 cgccgcggcc ggagacttca aaaatgccgt cctcgtcggt ttccttgacg cgcagcgcca
     9721 cgttggagcg caactctttc tgcagacggt cccagatctg gcggctggtg acgaacttgc
     9781 cttcgcggcc ggccagcggg ctggtgttga cgcagaagtt catggtcagc gtcggctcgt
     9841 cgatcttgag catgggcagg ggttgcgggt cggccacgtc ggtgatggtc tcgccgatgc
     9901 cgacgttctc gatgccgttg atcaacacga tctcggacgg accggcctcg gtcacctgca
     9961 cgcggtccag accctggaac tgatggatct ggttgatacg gcccttgtag ctgttgccct
    10021 ccgggccagc catgaccagc acgtcctggc ccgccttgag cgtgccctgg gtgatgcggc
    10081 ccacgccgat acggccgaca aaggtggagt agtccagcgc cgagacctgc atctgcaccg
    10141 gtgcggccgg gtcgcccttg atcgagggca catgcttgag cacggtgttg aacagggccg
    10201 acatgtcggg gccccactgt tcaccggggt taccctgctc cagcgcgctc cagccgttga
    10261 tgcccgaggc atagaccacg ggaaagtcga gctgctcgtc ggtggcgccc agcttgtcga
    10321 acagatcgaa ggcggcgttc accacgtagt cggggcgcgc gccgggcttg tccaccttgt
    10381 tcacgacgac gatgggcttc aagcccagcg ccagcgcctt cttggtcacg aagcgcgtct
    10441 gcggcatggg gccttcctgc gcgtcgatga gcagtaccac gccgtccacc attgagagcg
    10501 cgcgctccac ctcgccgcca aagtcggcgt ggccgggcgt gtcgaggatg ttgatgtgcg
    10561 tgccctccca gctcacggcg cagttcttgg ccaggatggt gatgccacgc tcgcgctcga
    10621 tggcgttgct gtccatcacc gtgtccacca ctttctcgtg ttgggcgaag gtgccggact
    10681 ggcgcagcag ctggtccacc atggtggtct tgccatggtc gacgtgggcg atgatggcga
    10741 tgttgcggat ttgtttgctc atagcgttgt tttccaaaat gccgcgtcct atgcgcgcgg
    10801 cgcgctttcc aatatctgtt gaatttccag ggggctcagc agcctgtccg ggatcagctc
    10861 accctgcgcg acatgagcca cgcccagcag cgccctgggg cgcgtgccat acacggccac
    10921 ggccggcgca tcggcccagg ggccacgccg gcgcaggccg gacagaaagc gcccggcctc
    10981 gctctcatcc agcgtgaccc tttcgtgctg cgcgagcagc gtgtccacgc tctgcacaca
    11041 ggccaggcgc tcgtcctcgg tcatggcctc gagtgcggcc agcgtcacgc agcgctgcac
    11101 gcccaggccg ccagtgtcaa tgcggcgcag aaacgtcagg tgcgcaccgc agcccagggc
    11161 ctgacctatg tcctcgccca gggtgcggat gtaggtgcct ttgctgcagg ttactattat
    11221 ttttatagct gtttgcgctt tacccacctg cgatacagcc agttctaatg catgaatggt
    11281 gacatcgcgc ggcgcccgct cgacctccac gccggcacgt gcgtattcgt acaacgcctt
    11341 gccgtccttc ttcagcgcac tgtgcatggg aggcacctgg cgtatggcgc cggtgaactg
    11401 cgcctgcacc tgcgccaggc gctcgggcgt gagctggcca gggtctacat cgcgccgctg
    11461 cagcacctcg ccttcggcat cgccggtgct ggtcgtgatg cccaacaggg cgactgcctc
    11521 gtaggtcttg ggtgcatcca gctgcagcgc gctgaacttg gtggccgcac caaagcacag
    11581 cggcagcacg ccggtcgcca gcgggtcgag cgtgccggtg tggccggcct tctcggcgcg
    11641 cagcaaccac ttcacctttt gcaaggcgtt gttgctggac aggcccaggg gtttgtcgag
    11701 cagcagcact ccgtgcaccg ggcgccgctg cacccggatg cggggagcgc gctcaatcat
    11761 tgccgtcgtc gtccttggaa cgcgaggcca cggcgcgcgc gatcagcgca ttcatgtcgg
    11821 ccgcgcgctc gggcgtgcgg tcgaacagga agtgcagcgt gggcacggtg tggatgtgca
    11881 ggcgcttgaa cagtccgttg cgcaaaaagc ccgcggcctg gttcagcgcc tcctgcgtgg
    11941 cctcggcatc gcccaccagc acgctgaaga acaccttggc atgcgcgtag tcgggcgtga
    12001 cctcgacggc ctgcagcgtg accatgccga tgcgcgggtc cttcagctcg cggatcagct
    12061 ccgcgaggtc gcgctggatc tgatccgaga cctggtggcc acggttgggt ttggtgcttc
    12121 gtttaggcat agggcatgtg gtgcgttaaa cgttgtgcgt tgggcgtgaa aaccacgcca
    12181 aacgcccaac gctcaaccgc ttcaaagcgt gcgcgcaatt tccttgatct caaagagttc
    12241 gagctggtcg ccctctttga tatcggtgta gttgcgcagt ttgataccgc actcgaagcc
    12301 ttccttgact tccttgacat cgtccttgaa gcggcgcacg gcctcgacct cgccggtata
    12361 gatgaccacg ttctcgcgca gcaggcggaa cctgcagccg cgcgtgacct ggcccgaggt
    12421 gatgtaggag ccggcaatgg tgccgatctt ggtcgccacg aacacggtgc ggatctcggc
    12481 cgtacccagc gcttcctcgc gctgctcggg cgccagcatg ccggacatcg ccgccttcaa
    12541 ctcatccacg gcgtcgtaga tgatgttgta gtaatgcaag gtcacatcgc tcgcctcggc
    12601 gtgcttgcgc gcattcgcgt cggcacgcac gttgaagccg atgatcagcg ccttggaggc
    12661 gatcgccagg ttcacgtccg actcgctgat gccgcccacg ccggcataca ccagctgcac
    12721 gcgcacctcg tcggtggaga gcttgagcag cgaggcggcc agcgcctcct gcgagccctg
    12781 ggcatcggcc ttgatgatga tgggcaggtg ctgcacctcg cccgcctgca tctcggcgaa
    12841 cacgttttcc atcttcgccg cctgctgctt ggccagcttg gtgtggcgga acttgccggc
    12901 gcggtaggtg gcgatctcgc gcgcacggcg ctcgtcggcc atcaccatga actcgtcgcc
    12961 tgcctgcggc acctcggaca agccctggat ttccaccgga atcgaggggc cggcttcctt
    13021 ggcggtctga ccgttttcat cgaccatggc gcgcacgcgg ccaaaggtct ggcccgccag
    13081 caccacatcg cccaccttca gcgtgccgga ctgcaccagc accgtggcca cggggccgcg
    13141 gcccttgtcc agctgacctt cgatcaccag gcccttggcc atggcttcca ccggcgcctt
    13201 cagttccagc acctcggcct gcagcagcac ctgctccaac aggtcgtcga tgcccatgcc
    13261 ggtcttggag gacacggcca cgaagggaga gtcgccgccg tactcttcgg gcacgacttc
    13321 ctcggccacc agctcctgct tgaccttctc cgggttggct tcgtgcttgt cggccttggt
    13381 gatggcgacg acgatgggca cccctgccgc cttggcgtgc ttgatggcct cgcgcgtctg
    13441 cggcatcacg ccgtcgtcgg ccgcgcagac caggatgacg atgtcggtgg cctgggcacc
    13501 gcgtgcacgc atggccgtga aggcctcgtg accgggggta tccaggaagg tgacgatgcc
    13561 gcgcggcgtc tcgacatggt aggcgccgat atgctgcgtg atgccaccgg cctcgcccac
    13621 cgccacacgc gagcggcgga tgtagtccag cagcgaggtc ttgccatggt ccacgtggcc
    13681 catgacggtg accaccggcg cgcgcggcag ggcctcggcc tgctggccgg agacttcctc
    13741 ggcggtgaag gcttcggggt cgtccagcgc cgccaccacg gccttgtggc ccatttcctc
    13801 gaccacgatc atggccgtgt cctggtccag cggctggttg atggtgacca tctggcccat
    13861 cttcatcagc gccttgatca cctcggaggc cttgatggcc atcttgtgcg ccaactcggc
    13921 gacggtgatg gtctcgggca catgcacctc gatggcgcgg aactcggccg gcgcctgctg
    13981 ctgctggcgc tcgtcgcggt cgttgccgcg gcggcccttg gggccggagc gccagttgcc
    14041 gcgccccacg ccgccgctgc tgtcgccgcg ggtcttgatt tccttcttct tggtcgcgtc
    14101 attggcccag ctggaagaca gcttggcgga cttcacttcc ttgccggcgc ccggcgcggc
    14161 cggcgcagcg gcacgggcac ccgccccggc ggccggcttg tgcagcgtgc ccttcttggc
    14221 gtccttggca tcggcggcct tggccgccgg cttgggctcc tcgggcttct tggccacgag
    14281 caccttcttc ggcgcggcca tcatggcgcg aatggcctcg gcctcggcca gggccttgcg
    14341 gcggcgctcg tccagatcga cggcacgcgc cgcctcttcg gccgcacgcg ccttggattc
    14401 ttcctcggcc ttggcacggg cctcggcctg tgcctcggct cgggcttgtg cctcggcaac
    14461 ggcctgctcc ttggcctcca ggcgcccggc cttttcctcg gccttcttct gctcctgctc
    14521 ggccacatag gctgcagcgc gctcttcggc ctcgcgctcg cgttgttcac gcgcctcgcg
    14581 ttcggcgcgc gactgtgcca gctcttcctc ctggcggcgg atcagctcgg cctgacggcg
    14641 ggcttcctcc tcgcgtcggg ccagctcctg ctcctgcgca atccgggcca ctgcggccgg
    14701 atcgggcgcg gcatcaacac cgtcctcgcg cttgacgaag gtgcgcttct tgcgcacttc
    14761 cacctggatc gtgcgtgcct tgcccgtggc atcggcctgc ttgatctcgc tggtggactt
    14821 tttcaccagg gtgatcttct tgcggtcgcc tgccgtgccg tggctggcct gcaggtaagc
    14881 cagcagtttc tgcttgtcag cctcgttcag cacatcggca gaggatgact tggccacgcc
    14941 agcggattgg agttgatcca gcagggtttc aggcgacttt ttgagttctg cagcgaactc
    15001 ggcaacagtg ttactcgaca tattctgttc gtacctccct gacctttact cttgccccgt
    15061 gaaccagtgc tcacgcgcct tcatgatcaa ggccgtggca tcctcggcag actggccggt
    15121 cagctcggtc agttcatcca ggccgagatc ggccaggtca tcacgtgtat tcaccccgcc
    15181 ggctgccaac ttggcgatca gctcgggcgt caggccttcg agatcgcgca aatcctgaga
    15241 cacggcgccc atgctttctt cgcgtgcgat ttccatggtc aagagcgcat ccttggcgcg
    15301 cgagcgcagc tcgttgaccg tgtcctcgtc gaagctctcg atctccagca tttcctgcag
    15361 cggcacatag gccacttctt ccaggctctc gaagccctcg gcgatcagga tgtcggcgat
    15421 ttcctcgtcc acatcgagct tttccatgaa caggcggcgt gcggcatcgc tttcatcggc
    15481 ctgcttctgc gcgctctcgg ccgcgtccat gatgttgatc ttccagcccg tcaagtcggc
    15541 cgccaggcgc acgttctggc caccacggcc gatggcgatg gccaggtttt cctcgtccac
    15601 aaccacatcc atggcgtgct tttcttcatc gacgacgatg gaggagacat tggccggggc
    15661 cagcgcgcca atcacgaact gcgccggatc gtccgaccac agcacgatgt ccacgcgctc
    15721 gccggcaagc tcgttggtaa cggcgttgac gcgcgtgcca cgcacgccga cgcaggtgcc
    15781 gatgggatct acacgcttgt cgtgcgagag cacggctatt ttggcccgac tgccggcatc
    15841 acgtgcgcag ctcttgatct ccagcaggcc ctgttcgatc tccggcacct cgttgcgaaa
    15901 caactccatc atgaactccg gcgccgagcg cgagaggatg atgggcgcgc cgcgcagcgt
    15961 cagatccaca tccatgatca tggcgcgcac gcggtcgcca ttgcgcaggt tttccttggg
    16021 gatcatctcg ccacggcgca ggcggccttc gacacggccg gactcgacga tgaggtcgcc
    16081 cttgtccatg cgcttgaccg taccggtaaa gatcttctcg ccacggctca tgaagtcgct
    16141 cagcagcatc tcacgctcgg cgtcgcggat cttctgcagg atgacctgct tggctgccat
    16201 ggcgccgatg cgccctatgg gcacggactc gacctgctcc tcgatgtact cgccaacctc
    16261 gatatcggcg atgcgctcgc gcgcatccat gagcagttcc tcagcgtcgg gattctgcag
    16321 gccggcctcg tcgggcacga ccagccagcg gcggaaggtt tcgtattcgc cgctatcgcg
    16381 atcgatggat acacgaatgt ccacctcgcc ctggtagagc ttcttcgtgg cctgcgccag
    16441 ggccgactcc acggcgccca tgaccacatc gtgctccacg ttcttttcgc gtgaaatagc
    16501 ctcaaccaac atcaacagtt cgcgattcat gcgacgactc tcctgtttct caatccctgc
    16561 cgggggctgc cgtggcggca ttcccgaata ctcaaatttt tagtccgcgg aatctggctt
    16621 ggcgccacgc cccttgaagt ccacgatggg cgcaagccgc gcctcacgca actcatccag
    16681 cgtgaagccc agcgcctgca gcggggccgg cacgcgcttc ttgctcacgc gctggcccgg
    16741 cttgaccggc ggtgcgtcgc tccagacgat ctgccagccg ccaccgtcca cacgctcgag
    16801 cgtgccgcga aatttcttgc ggttggccgc cacctgccca ccagccgccg cgcccagggg
    16861 ctccttcaag gtgatgtcga ccatctcgcc ctcgaagcgc atgaaatcct gttcgttgcg
    16921 cagcagccgg tctattccgg gcgaggacac ttccaggcgc ttgtagtcca ccccttcgac
    16981 ctccagggcg tactgcaact ggcgcgtcac cttctcgcag tcctctacgg tgatgaactg
    17041 ctccaccacc ggctcctgga cctcggccgc aggcgcagcc cacaccagat cgatggtgat
    17101 gcgcagcaat ccccctgcgg agcgctcgat ctccaccagg tcatagccta acccggccac
    17161 ggtttgttca acgatttgct gcaatgccac aggtgtgcgc tctgtccaat tccaaatgaa
    17221 aagcctggcc gatccaaaac ccctggagca gccaccagcc aaaaagcaaa cgcccaaaaa
    17281 aaacgggcgg tgagtacccg cccgttttgg tcgtgaagct cgcattgtat ccgctaatgc
    17341 agcactatgc aaaatccagc ggcacgcatc ttgcaatgct gcgtcagcgc accctgctcg
    17401 cggcgatgcg ctcgaacagc gcctgcgcct gcgcatttgc ttcgggcgca tctggatctg
    17461 cctgcaggct ttgcagcatg cggcccagca cctgagtttg aggaagcacc gtgcccagaa
    17521 aacgggcagc gccaccatcg gcgatcaaaa ccgtggggaa tgtttccaca tccacatcgc
    17581 ccatcaggtc ttcctcgtcc tccacgtcca cccactcaaa gcgcacctgc ggccagtcgc
    17641 gcgccaatgc gtcgaacgcg ctgcgatact cacggcacac gccgcaccat tgggcgcaca
    17701 gacagaccac ccaccagggt tggcacacgc cggcagcgga ttcagtgtct tgcaaaatgg
    17761 cctccatacc atgcaatcaa gcgctcgtat tcaagagcca cgccgttcac gggcgcggta
    17821 aacatctatc gtatcgaccc tgggcgccac attgccccag gcattgcgga tataggacag
    17881 cacggccgcc atttcctcgt cacgcagact ctgcgcaaag ggtggcatgc catgagggcg
    17941 tggattgccg gcggtcgccg gttggtaccc gccctgcagc aatacgcgca ccaggttggt
    18001 ggtatcggca agcgtcacgg cacggttgcc agccagcgcc ggaaaagcgc cgcggcgccc
    18061 ctccccctgt tcgccatggc attcggcgca ctgctgccga tagatatctt gcccgcgctc
    18121 catcaggtcg gccgcaggct tggcgacagg cggctcgggc ttgtcctgcg gcggcaggct
    18181 gcgcagatat accgccatcg cgcgcagatc ggcatcgctc aggtattgca ggctgcgaaa
    18241 caccacctcc gccatcgggc ccgacacacc ggcctggagc gaaacgcccg tcttcaacaa
    18301 agccaccaca tcctccacgg gccaggcggt gacagctgcc tcgcgcccgg agttgagtgc
    18361 cggcgcatac cagttctgcg cgggaatcaa gccgccatgg aactgcgcgt cggtgcgcaa
    18421 tgcgcccagg gcattgcgtg gcgtatggca ggcagcgcaa tgccccaggc cctgcaccag
    18481 gtaggcacca cggttccact ctgctgactg cgtgctctgc tgcacaaacg taccagggcg
    18541 aaagaacaag gcgcgccatc cggccaacgc ccattgggtg ttgaacggaa agcgcagtac
    18601 atgcggtgga ctttgcaatg gcactggcga caggctgcgc aagaaggcgt agatggcatc
    18661 cgaatccgcg cgcgtgatgt aggtatagct ggggtaagga aacgcaggat agagcagccg
    18721 cccgtcacgc gaacgtccgt tgtgcatggc gcgccagaat tcggcggccg accaacggcc
    18781 caggcccgtc gcatcgtcgg gcgtgaggtt gggtgcatag accgatccaa agggtgtagc
    18841 gatggcccgt ccgccagcaa atgccggacc gcccggctgg gtatggcatg acatgcagtt
    18901 gcccacgcgc accagatact caccctgggc aatcaacccg gggctagccg gtgcggcggc
    18961 ggcctcgcgc tcgggtaatg gcagttcatc caaccgattc agcgccacca gggcagtcgc
    19021 agccaacaga atcagcaagg tggcgccagc cccccataca agccaggcgc ggcccatgct
    19081 caccgcgcgc tccctgcctc ggctggggcg gcaacccctc cacaagggat gggcagtttt
    19141 cccttgaatt cagctgcagg cttgccctgc tccggcacaa cctgggccgc aagccaggta
    19201 gccacggcat gcacatcccc tggggccatc tggcgcgcaa tgcgggccat gcagtcaggc
    19261 gattgagcgc gccgctgccc cgcctgccag gcccccaact ggctgctcac atagtcacgc
    19321 gacagcccca gcagaccggg aacaaagggc gccacaccgg tcagggcgtc gccatggcat
    19381 tgcgcgcagg cggggatttt ttggctggca tcaccgtggc gcaccagttg ctgcccacgc
    19441 tcaagcaagg cccgcgctgc cgatgtggcc gcaggtggtg catatggcaa gtccaatgct
    19501 gcgaaatacg cagccatctc gcgcaggtag tcatccgtca gatgctctag cagataggac
    19561 atctgcggat agctccgccg cccgtcacga aaattgatca gctggtggta caggtagcct
    19621 gccggcttgc cggcgatgcg cggaaagtag ccgctgggcg tggcccggcc ctccttgcca
    19681 tggcaagccg tgcaggccag cacccgagcc accataccgt catcatcaaa tgccgcctgc
    19741 accgttgtcg ccaagagtcc caggcaaaga gccagacaaa ggcgcgccaa gctgaccatg
    19801 tgtgctcacc cacgtccggc aaagggcatc ttcgtggcca tcacggtgtg gaacatgacg
    19861 ttggcctcca gcggcaggtt ggccatgtac agcacgcttt ggccgacgat ggccacatcc
    19921 atcaagggct cggccgcaat ctggccgttg gcctgctgca ccccctgcgt catgcgctgg
    19981 gccagttcgg tctccgcgtt gcctacatcg atctgaccca cggcaatatc gtacttacgc
    20041 ccgtccaggg atgcggtctt ggtcatgccc atcacggcat gcttggtcgc ggtgtaggcg
    20101 acggagttgg gccgcggagt atgcgccgag atggagccgt tgttgatgat gcgcccgccg
    20161 cgcggcgact gcgacttcat cacgcgaaag gcgttctgca tgcaataaaa catgccgctg
    20221 aggttgatgt ccaccacatc tttccactgc tgcaccgaca gatcttccag cggtatcgcg
    20281 ggcgcgccca cgccggcgtt gttgaacagc agatccacgc ggccgaactg ctgcaccgtg
    20341 cggtcgaaca gctggcgcac tgcctcgggg tcggcgacat ccgtgggaac ggccagggcg
    20401 cgcgtacctg cgccggactc cagcgccacc tgcaacagcg gctcttcgcg ccgacccgcc
    20461 agggccacgc tccagccatc cctgagcaag gccaatgccg cggccttgcc tatgcccgtg
    20521 ccggcacccg tgacgatggc gattcttaca gtctgcgtca tgtctgatct ccgattgctg
    20581 cgactcaagg gcgcggcttg cgcccttttg cgcctggttt tccaccggcc ttgccgccgt
    20641 ttctggactc actcttgccg gcgccatgct tcttgccgga gccgccgtgg ccgtgatcct
    20701 tgtctttgtg gccacgcatg tcatggctcc tggcaaagcc ttggggctgc gaggcatccc
    20761 tcaagcgcgc atcctgctcg ggggggcgct tgccgccatc gcgcgccttg gctggcttgc
    20821 cctgcaccgt ctcgcccgtt tcatccacca ggcgaaagtc gatcttgcgg ccatccagat
    20881 ccacgcggct cacctggacg cgcacgcgcg caccaatcga gtagcgaata cccgtgcgct
    20941 cgccacgcag ctcctggcgc gcttcgtcga acttgaagta ctcgccaccc agctcggtga
    21001 tgtgcaccag gccctcgaca tacagggcat ccagcgtcac gaagatgccg aaactggtca
    21061 cggcgctgac cacgccgctg tactcctcgc ccaggtgctc gcgcatgtac tggcacttga
    21121 gccatgcctc cacgtcgcgg ctggcctcgt cggcccgacg ctcgttggcg ctgcaatgca
    21181 ggcccgcggc ctcccaggcc tgggtgtcgc gcacgctgat ggcgcccttg cctggcctga
    21241 tcgccggttc ggccaccttg gaggccaggc gcttggccag cttgaactgc gcctcgccag
    21301 gcgtgggcaa ggccggaagc gcgtacttgg tgccggccag tatggacttg atgacacggt
    21361 gcaccagcaa gtcagggtag cggcgaatgg ggctggtgaa atgcgtgtag gcctcgaacg
    21421 ccaggccaaa gtggccactg ttgatgggcg tgtagatcgc ctgctgcatg gagcgcagca
    21481 gcatggtgtg gatctgctgc gcatcgggcc tgtccttggt ggcctcggca atggcctgga
    21541 actcctcggg acgcgggtcg tcactgatcg acatgcccac gcccatggcc ttcagatagc
    21601 cgcgcaggat gtcctgcttt tccggcgtcg gcccctcgtg cacacggtac agcgccggac
    21661 gatgcgcctg ctcgacaaag tctgcactgc agacattggc cgccagcatg gcctcctcga
    21721 tcagcctgtg ggcctcggtg cgcacgcgcg gcacgatctt gtcgatgcgg ccgttgtcgt
    21781 cgcaaatgat ctgcgtctct gtggtctcga agtccactgc gccacgcgcc tggcgtgcag
    21841 tcagcaaggc gcgatagcag tcgtgcaggt tcagcagatt gcccacgcga tcctggtatt
    21901 tcgctgcctc cggcccacgc gtattcccca gcacggccga aacctcgttg taggtcagcc
    21961 gggcatggct gagcatcacg gccggataga actgataggc ctgtacctcg cccttggcac
    22021 tgaccatcat gtcgcaaacc atgcacaggc gctcgacatg cgggttcagc gagcacaggc
    22081 cgttggagag tttctccggc agcatcggaa tcacgcgccg cggaaaatac acgctggtgg
    22141 cgcggtcgta ggcgtcgata tcgatgtcac tgccggtttc cacatagtgg ctcacgtcag
    22201 caatggctac caacaggcgc cagcccttgc cgcgcccgac cttgacgggc tcgcaataca
    22261 cggcatcgtc aaagtcgcgg gcatcctcgc cgtcgatggt gacgagtgga atatccgtga
    22321 gatcgacgcg ccggcgctta tcgagcgcgc gcaccgtgtc gggcagcgcc ttggcctgcg
    22381 ccagacaggc ctcagaaaac tcatgcggca cgccatattt gcgcaccgcg atctccacct
    22441 ccatgccggg atcgtccacc tcgcccaaca cttcgaccac gcgccccacg ggctgaccaa
    22501 acagggccgg cggctcggtc aactgcacca ccaccacctg gccggtggca gcggatcccg
    22561 tggcgccctt ggggatcagt acgtcctggc cgtagcgctt gtcctcgggg gccaccagcc
    22621 aaacgccact ttcctgcaac agccggccga tgatgggttg cggtggccgt tcaatgatct
    22681 ccagcacacg cccctcgggc cgaccacgct tgtcctggcg cacgatgcgc acacgcaccc
    22741 tatccttgtg caacacggcg cgcatttcgt tggggggaat gtagatgtcc ggctcaccat
    22801 catcgcgaat gacgaaccca tgcccgtcac ggtgaccttg cacggagccc tcgatctcat
    22861 ccgaagaaat ttgcgccgcg gacgggccgg agtatttttt tttgctatac aatctatttc
    22921 tttcctgaaa tgcccaggtg gcggaattgg tagacgcact agtttcaggt actagcgctg
    22981 agaggcgtgg aggttcgagt cctctcctgg gcaccaagtt taacgagaac ccgcaggggt
    23041 tttgagctca aaggccgctt tcctcgaaag cggccttttt gtttttgcca acgcccggaa
    23101 agctggcgca acgcaaacca actcaggcgg gtcgctcgca aaaatatcaa caaactttct
    23161 ttgactccta aaaatcgcat tacaatgcga gcttccctga aatgcccaga tggcggaatt
    23221 ggtagacgca ctagtttcag gtactagcgc tgagaggcgt ggaggttcga gtcctcttct
    23281 gggcaccaaa atagaacaaa ggccgctgat gaacatcagc ggcctttttg tttgcactcc
    23341 cttggcgcac aagcatcgcg gcggcaacac agtgccgccg cacacaaagc ccgctacttg
    23401 cgcagcaaaa ccttgagcac gctttgcagc cgacgggcgc tggcctcatc actggcgccg
    23461 gcaagcacct gggcagcatc ctgcgcccag gtctgtgccg gagccggatc attgcggcgc
    23521 gtgagcattt gcagctgcag ggcgcggcga gcagccagtt gatcggctgg cgtgggtaca
    23581 tcggcggcca tttccaggcg caatagagcc tgttcagtac ccgtacctgc cggggctgcg
    23641 gcaatggcct gcccccaagc ggcgcgcacg ccggcggaca ccttgccacc gagctcctgc
    23701 aacgtaggca gctgctcggc atcgcggctc ttccaggccg ccatcagttg ggtgagcgcc
    23761 tcgccatgcg cctgggcggc cagtttgcgc aacgcggttt gtgcatgctc catggcatcg
    23821 cgctgggccc ggaaggcggt atcgcccagg cgcggtccgc gtgcttcaaa agcttcgcgc
    23881 tcgcggccac gccccgcgcc atcgcgcggg ccacggccgt cgcgcgcatc acggcgctca
    23941 ctgcctgggc ggccggcacg tgccggttgc gccgactcgg ccttgcgcat gcccggcctg
    24001 tcgtcgccac gcaccgccac caccggtcgc acgggagcgg cagcaacagg cttggcactc
    24061 tcctgattta cttctgaatt tatagctgtt tgcgcttgat ccgcctgcgt ttggggctga
    24121 tttgcatcag attcgactgc cttggcctgt tccttgatgg ccaattccag cgccgccatt
    24181 gcagcgcgaa tctgttgcgc atcgcccgaa gcgctggcag cctccagcgc cttggaggcg
    24241 tccagcacgg cgcggtcgcg cgcactcaac tgctcggcac tgcgctcacg ctcgactgtc
    24301 ttgcggttga atgcatcatc aatgggcttg cgaaaagcgt cccacagcct ttgctcatgc
    24361 ttgcgatcca gtggcacgga ctgggcctct gcctgccagc gctgctgcag cgccttgatg
    24421 gcgtcaatgc gcagtgaagc cgccgcgccc agggcctcgg cctcggcaat catggcctgg
    24481 cgcagctgca ggctggcctt ttgcgcagtc tccagcggcg cagcagccgc gcccagcgcc
    24541 tgcttccaaa gcggctgcag ctcggcaaac accttttcac tcacatggcc gcctgcgcgc
    24601 cagcgctcgc caaactggtg cagggcacgg ttcacggcct tccagtcggc cgccgccgcg
    24661 gccccctgct cctgcgccca ggtggtcact tcttcaatca gggccatgcg ctgcgcgcgg
    24721 tgctcaaccc cttcggcgcg caccttttcc agccaggatt cgaccacctt gtgggcggcg
    24781 ttgcaggcat cgtcaaactt tttccacagt ccatggttgg ccggtgcgcc ctggtcggcc
    24841 tgcttccact gctcgcgcag ctggcgcagg gtttcctgca tcttgcggcc acccagggcc
    24901 tggccatcgg ggcgacgcag caacgcctcg gctcgggcca ccaggtcttc gcgcacctgg
    24961 tcggcgctcc agcgctgcca gccctcgagt tcgccggcag ctaccagggc cgcatgcacc
    25021 ttgcgctcca gctctgcgtc gatgaaacgg ccatgcggct tgagtatcgc gcgcagcgcc
    25081 gcagccgcgc cggcactggc cttgccatgc ccttgggcag tctcctgctc cagcttggcc
    25141 aaggcctcgc tcacggcctg cacggccttg tcgcgcacct cgggatcgac cttgggtttg
    25201 gctgcggtac gggccggcgc gctggcggcg gcctcggccg gcaagccgcg cgcctgacgc
    25261 agctcatcgg cccacactgg cacgggcggc aggggcgccg ccgcgtcctg ggcagcggcc
    25321 acggtcaatg ccagggccga ctgaaacgcc tcccagacca ccagcaactg gctgcgcgaa
    25381 gactccagtt gcgttggaaa gcgcgcctcc acgctgggcc aggcagcatc gcccgtcagc
    25441 acctgtgcct gctcttgcca acggcccaca tcagcgctca aggcctccag tgccgcctgg
    25501 gcgtcctgcc agggcttggt ggacagcacc tcgatgcgct gtgccaacag caccgccgcc
    25561 tcacgctgca cctgcacacg gtgctgcagg tcttcaatgg tcttcacgcg ctcactcagc
    25621 tgcgccttga gcagagacag cggctcgcgc gacagtgcgg cgccggcctt ggccgcgtca
    25681 cgctgccagg ccagggcatc ggcaatgttc agctgggccg cggccagcaa cgcctcggcc
    25741 ttggcctgcc attcggcggc aatgaccgcc tgcccctgcg cacgccggat ttcgtcgagc
    25801 cgctcgcgca ccgcccgggc cgcgcccttg tcgcgggcgc tgagctcctt gaagacctcc
    25861 tgcaactgat cggtggcggg ttgcgtgaca agccactcac ggatacgcgc cgagcgctcg
    25921 ccggacgtgg cggccgagaa agcgccgccc gtcagagcat ccaggggatg cggctcggtg
    25981 gtcttggttt gaaccggggt cgcttcgggt gcgtcggaca ttttgctgcg agaagaaaaa
    26041 gggaacatgg attcatcgag aaaaatggcg ggctgcctgc tgcagcccgc accatgccag
    26101 gcgaagagcc cacggcccat gcttggcaaa gctgctattt tcgcctcaat tcagagcgca
    26161 gcctttatct ggagcaagtc aggccaatgg cagcaccaaa attggccgcc gccgtactcg
    26221 gcatggcgac acaagccggc actgcctgcg ctcaacgcgc cgccaacgcc agctccgcct
    26281 tgggcgccca gctggcccca ccctcgcccg ccggctgcag cttcgcggcc cccacgaaca
    26341 accgatccag cggcacctgc cggctggcga ggtagtcgcg cacggcgacg ccgcgcgcca
    26401 gggccagatc ctgcatggcg ttgtccggca cggtgatgct cttgagcagc agcgcctcca
    26461 tctccggtag cggcaaatcc ttggccatgc cgaccaggtt gcgcggcttc ttgatgtcgg
    26521 agcgctgata ggcctccttg agcagggccg gatattccgc atcggtcact ggcgccacgt
    26581 cggcggccga ctcgcccgcg cgcgcggcgt cgcggcgctt ctgtgcctgt gccagatcgc
    26641 gcaggcgaag gcgcttccag gcctcctgct ccgcctgcgg ctgggcccag ccgaccacgg
    26701 tgagcttgag tgccggccga ttgaccagct gctcggcaat tttgtctagc agctggtgcg
    26761 ccttgtcatc cagtgcgctg ctgccctgga cgaagtgcac cacaccctgc tcgtccgcag
    26821 cgccgaacat gccggcaagc aaggaaaacg gcgcggtaac cgccttcatc acgaggttgc
    26881 cgatcacctt caggataacg ccgccgaggc tgaattgcgg gtcgttgagc gagccgctga
    26941 tgggcagatc caagtcaatg accccattgc ggtcggccag caaggcgacg gccagacgca
    27001 ccggcaggct ggccggcgcg ccttccaccg cgtcgccaaa agccagttgg ttgagtacca
    27061 gcttgttatt cgccgtgagc tggccattgg gctgcacctt atacgacacg tccatgctca
    27121 gcttgccgcg ctcaatgcca tggcctgagt acttgatgct gtagggcgtc aggggcggca
    27181 gctccaggtc acgcatgcgg ccctgaatat cgagggccag cggcttggcc aaggggttca
    27241 gcttgccgga gacctccagc gaggccgtgc cctgcgcgcg gccgcgcagc tccagatcgg
    27301 ccatttgcaa tgcaccgccg gcttgtgcag gcaccgacga aaacgcactc aacctgccgc
    27361 tcagctcgct caggtcggcc gaatagttgg gcttgatgaa aaagtcggag aaacgcaccg
    27421 acccattggt cagcgccacc ggcccgaacc ggatcaccgg cgccatgggg tcggccggct
    27481 gcgccgctgg ggtttgcgcg gcctcgcctg gtgctgctgt tggcgtagat gctgcctgca
    27541 ccgatggcgc ggccaccctc gactcggcag ccgcgccctt ctggatgtcc tgcaggttga
    27601 tacggccgtt ctcatgcacg atgacacggg caaagaaatc actcagcgcc gtttccttca
    27661 cgtccagcgc caggggctgg cctggtttca ggttcacggc caggccacgc agcgccaggg
    27721 atttccagcg cagcaactcc tcgccacgct gagcgatccg catgccaccc tcgtcctgtc
    27781 catcgccggc atcttcgtcc ttggcgcgca cgcgcacctc gtccagcgag gcgtcgcccg
    27841 ccacggccac caacgggccg gccttgtcct gcacgtagcg cagactgccg gtaaaactgc
    27901 cgtcggcgcg caggatgtcc acattcaagt ccgcggtgac atagggctcg aacgcctgca
    27961 agggcaggtg ctgcgcgcgc agttttccct gcacgctcag ggccggattc atggcgaccg
    28021 agccctccca attcaagcgg cccggctcga tccggcctgc ggccacacgg gtatccaggc
    28081 gcacggcggc gggcgcgagg cggccatcgg cgaaggcgac atcccgcaga tgcaactgca
    28141 aaccggacgc attgagtgcc accgggcgct ccggcacctc atcacgaaag gcaatactgg
    28201 caccgtccac atccagcgcc gcaagcttca gtgcccaggg cgcctcaacc ttcttcggta
    28261 cggcatggtt gggctgttgc tctggtgcct gagccagcca ctgctcaaac atccaatcgc
    28321 ccttggggcc acgggacagc agcatcctgg gttggtgcaa caacaggcgc cccaccgtgg
    28381 cacgctgttc gggcagcatg accagggcat ccgtcacctc aaggcgcccc agttcggcca
    28441 cggacgtctt gcccgcatcc gcaatacctg ccctgcgcag gcttgggcat ttgtcctcgg
    28501 cggcgcaggt cagtaccaga ttgtcgagtg acagcgttgc cacctgggcc accatgacag
    28561 caccattcct ggccaggcca atgtcggcat cgagcatgcc acccaatgca ggcttgagca
    28621 cggccgcaag atatggcgcg gccatggcca gcggcaagcc gcgaatggag gctgccgcct
    28681 gcgcctctgc atcggtagcc tgcccgcgca gcgccaccca ggccggatct cccttggccc
    28741 ccccgccgga caaccgcgcg ctggcgcgaa actgcagcgg ctgcttgaag ggcagttcga
    28801 tggccgaagc ttgtagttgc aaattttctg ccttccatac ggccgtgccg cccggcaggc
    28861 tggcatctac ccaatccacg gtagcgtcat gtacgcccag gcgcgccagc cgcacctgcc
    28921 agggcgattc ctgtacccct gcggttggtg gggttttgac gctggcgtca tccaccggtt
    28981 gattcactcc gggcagggtc aacgcaccct tggcatcccg gccgatgtgg acatggacac
    29041 cactccaatc gatggcagca atgtccacga tgcgccgcag aggttgcacc tcgttgagag
    29101 ccacctgcaa ggaatcaaag ctcagcaatg gttgccccgc aacatccagg gtctgtatcc
    29161 cctgcaactg tgcactgcca ctgatcttga cctggggcgt ggcatcgacc tgttcaaagt
    29221 ccagcttcaa atcggcattc agcgtgccgg ccctgagccg caccggcacc gagccgggga
    29281 tgtagccgct gaagggggca agatcaaaac cgtggatctg caggtgcaga tcggtctggc
    29341 gattctgcgc aaacggagtg gcctgcgcgg ccgaatcgaa ggcgctgcca ttgagatcga
    29401 atgccaggcg cggaatgacc cggatttcgc ggtctgcagc aaagttgctc aagaacggca
    29461 gttccagctt caactggcgc agttggtgga ctttgtccac cgtgcggtca ttgaaatcga
    29521 tggccccccc ctgcaacgca atgttgtaca gcgcaaagcg cagtggtgac gcaggctctt
    29581 catccggctt gcgctgctgc gccaggtgtg ccagtacgtc atcaaagtca aactcgccag
    29641 gggcggtctg ggtgatatgg atcacggggt catcgacctg cagagcgtcc aacaccggag
    29701 ccaggcgcca tagggattgc aactctgcat ctatatagat acgtgcgatc tgcacctggg
    29761 gcgactggcc atcctgcgac gcgatggaca gcccgcgcac ggtcgcctcc agcgaccagg
    29821 gcgcaaactc caccttcgcc accgtgaccc tgcggcccag cgcctcggac gcgtgctttt
    29881 cgatctgcgc acgcgcaatc ggcgggaccg ccagccaggc cagcagccac agccccagca
    29941 gtccggccac aagccaggtg gcacgccggg cccagcgatt gcttttgatg gtttgtatgg
    30001 cttgtgacac gcggcaccca atccctggaa caacaggcgc aattgaatca gcgaacacct
    30061 ggacgccaat gtaaaagagt gcaaaaagaa agcccggtga acggagtcct tccgtacacc
    30121 gggcttgacg gcagacgcaa cggtcttttg ccatcttttg cgtcgaggag aggagcgtcc
    30181 ccgacgcagg aggcaaggta ttgcctccag gggttgcctt ggaagcgagg tacctgttgc
    30241 tcggtgtggc actgactgcc ttccaaccag cggtttcgcc ccttccggcg tacgcctgaa
    30301 tcgttcaggc agccctaagg tagcaaatac caccggcgct ttccagcagc ttctttcaat
    30361 gagcccgcca atgcgattgc cactttcatt tggccgctta agccgcaccc cgctacatgg
    30421 cccccaaggt gctagaaaag tcacatttcc gaatctatta actattaaca gtatgttaat
    30481 tatttttttt tatataatat gcaatactta gtattgactt ggcatttaga ccccctctag
    30541 aatccgccat tcttgaaaaa cagaccgtac aaaccctagc cgtcacccga caccatctag
    30601 gagagtccgc aagcttccat gacgcagcgc gccatggcac ggaccgaatg gcagaccaat
    30661 ccaagccagc aagctggcaa gaccgagtcc tgctgcaccc cctgccggcg cagcgctcca
    30721 ctgggctcga ctgagaaagg aaaaacgatg acagcgtcag gaaatgcgat tgcaagtgcg
    30781 cgcacctttg ccgccgatgt gatcaacggc tttcttgaag tcacacacaa cggcttcgcc
    30841 ctgctcgggc tggccgtggc ctttgtggcc atcgccctga gcgcacgccc ggacctgcgt
    30901 caggctggcg aggacgagtt gcgcggctgg ctgcatgaac gccgcgtcgc agaagtcggc
    30961 ttcccactgg agccgaccgc cagcgaacgc gccgtggcca tcaaccccaa gaagttgccg
    31021 cgcgaacagg cggccgtggc cttttggctg agcaagaagt accgcgtggc cccggagccg
    31081 ctggctgccc tggtgaccga ggcctatgag atcggcaagc gcaccaagct ggatccgacc
    31141 ctgatcctgg ccatcatcgc catcgagtcc agcttcaacc cgtttgcgca aagctccgtc
    31201 ggcgcacaag gcctgatgca ggtgatgacc agcgtgcaca ccgacaagta cgaaggtttt
    31261 ggtggtcagc tggccgcttt cgaccccgtg gccaacctgc gtgtgggcgt gaaggtgctg
    31321 caggagtgca ttgcgcgcgc cggctcgctg gaaggcggcc tgcgctacta cgtgggcgcc
    31381 gccaacctgc cccacgatgg tggctacgct gccaaggtgt tggccgagca tttccgcctg
    31441 cgccaggtag ccgggcggcc cgcggctgcc accccggtgc tgtccaccaa ggccccggcg
    31501 gccaccgaga tcgccccaac agccaagcct gccgaagaaa aagtggccct gctcgacagc
    31561 gacgcctgaa ttcccggcgc tggcgcgaag caccgtccgc tacactagcg gggcacgcaa
    31621 ctggcgatag gcgcgcaagg cccaccgggc tgcgctgctg acgtggagac ccgcaaaaac
    31681 aagccatttt ttgcaaacca ctggggagcg tgccgcgcgg aacaaggcag cgtttgcccc
    31741 gccgcgatcc gtcgttcgcc tgggcagccc ttgatttcca agggactctt agttcatcgg
    31801 actgccatgt accaacgcaa tattcttgtc gaacaaaccg atcccgaggt gtgggccgcc
    31861 atccaggccg agaacctgcg ccaggaagag cacatcgagc tgatcgccag cgagaactac
    31921 gcctcgcccg ccgtcatggc ggcacaaggc tcgcagctga ccaacaaata tgccgagggc
    31981 taccccggca agcgctacta cggcggctgc gaaaacgtcg acgtgatcga gcagctggcg
    32041 attgaccgca tcaagcagct gttcggtgcc gaggccgcaa acgtgcagcc caactcgggc
    32101 tcgcaggcca accaggccgt gctgatggcc ttcctcaaac ccggcgacac cattttgggc
    32161 atgagcctgg ccgaaggcgg gcacctgacg cacggcatgg cgctgaacat gtcgggcaag
    32221 tggttcaacg tggtctccta cggcctgaac gacaaggaag agatcgacta cgacgccttc
    32281 gaagccaagg cgcgcgaaca caagcccaag ctgatcatcg gcggcgcatc ggcctacgcc
    32341 ctgcgcattg atttcgagcg catggcccgc gtggccaagg aaatcggcgc catcttctgg
    32401 gtggacattg cccactatgc cggcctggtg gtggcgggcg agtaccccaa cccggtgccg
    32461 tttgccgacg tggtgaccag caccacgcac aagagcctgc gcggcccgcg cggcggcatc
    32521 atcttgatga aggccgagca cgagaaggcc atcaacagcg ccatcttccc cggcctgcag
    32581 ggcggcccgc tggagcacgt gatcgcagcc aaggccgtgg ccttcaagga ggcgctgtcg
    32641 cccgagttca agacttacca acagcaagtg gccaagaacg ccaaggtgtt tgccgagaca
    32701 ttgacacagc gcggcctgcg catcgtcagc ggccgcaccg agagccacgt catgctggtc
    32761 gacctgcgcg ccaagggcat caccggcaag gccgccgagg cggccctggg caaggcgcat
    32821 atcaccatca acaagaacgc catccctaac gaccccgaga agcccatggt gaccagcggc
    32881 attcgcgtgg gcacgccggc catcaccacg cgcggtttca aggaagagga aacgcgcatc
    32941 accgccaacc tgctggcgga cgtgctcgag aaccccaatg acgaggccca cctggccgcc
    33001 gtgcgcgaga aggtcaacgc gttgaccagt cgcttcccgg tctatcgctg agctgcgcca
    33061 tgaagtgccc gttctgcagc aatcaggaca cccaggtcgt cgagacacgg gtgtccgagg
    33121 acgggaactt catccgccgc aggcgccaat gcggtgcctg cgaaaaacgc ttcaccacct
    33181 atgagcggcc ggacgttcac ttccccaccg tggtcaagaa agatggccgg cgtgtggagt
    33241 attcgcgcga caagctgctc gcctccttca agctggcgct gcgcaagcgg ccggtgagca
    33301 cggtgcagat cgattcggcc atcgagcgca tcgaagagaa actgctgcaa ctgggccagc
    33361 gcgaggtgat ctccagccgc atcggcgagc tggtgatgcg cgagctcaag aagctcgaca
    33421 aggtggccta catccgctat gccagtgtgt accgcagctt cgaggacatt gatgagttcc
    33481 gcgctctcgt cgatgaagtc cgcaagtagt tcatcgcttc tctattgata gctgtcagcg
    33541 cccatagcct gggcgctaca gccatttttc acttaaacct ctcgcgcgga cgcaatcggc
    33601 ggcacggcct gccgcacgtc gccgcactgg gcacggtggc gcagcgcgtg atccatcagc
    33661 accagggcca gcagcgcctc ggcaatcggc gcggcacgta tgcccacaca gggatcgtgg
    33721 cgccccttgg taatcacctc ggtgctctgg ccgtggatgt ctatggactc gcgcggactg
    33781 atgatggagc tggtcggctt gatggccagc tgcacctcga tgtcctgccc ggtgctgatg
    33841 ccgcccagca ccccgccggc gttgttgctg gcaaagccct gcggcgtgag cgagtcgcca
    33901 tgcacggtac cgcgctgggc cacgctggca aaaccggcgc ctatctccac gcccttgacg
    33961 gcgttcaggc ccatcatcac gtaggcgatg tcggcgtcga gcttgtcata caaaggctcg
    34021 cccaggccga ccggcacgcc ggtggcctgc acgcggatgc gcgcgccgca ggagtcgccg
    34081 gacttgcgta gcgcgtccat gtagtcctcg taggcctgca catcggccac cggagcaaag
    34141 aaggggttgc gcggcacatg ctcccagctc tcgaaaggga tggcctgctc gcccagctgc
    34201 gtcatgcagg cgcgaaacgc cgtgccgtat ttctcggcca gccatttttt ggccacggcg
    34261 ccggcggcca cggtgggcgc cgtcaggcgc gccgacgagc ggccgccgcc gcgcgggtcg
    34321 cgcaggccgt acttgtgcca ataggcgtag tcggcatgac cggggcgaaa gctctgcgcg
    34381 atgtcgccat agtccttgct gcgctgatcc tggttgcgga tcagcagcgc gatgggcgtg
    34441 ccggtggtct tgccctggta cacgccggag aggatctcca ccgcgtccgg ttcatttcgc
    34501 tgcgtgacat ggcggctggt gccggggcgg cggcgatcca gatcctgctg gatgtcggcc
    34561 tctgacagct ccatgcccgg cgggcagccg tcgatcacgc agccaatggc cgggccatgg
    34621 gattcaccga agttggtgac gcaaaacagg gtgcctaagg tgttgccgct catgggaacg
    34681 cgatgctctt gaaaccgttg aaactataaa aacaatagct gtttgcgctt tatacataag
    34741 cgcaaacggc tgattttgtt caaacctcaa gctgccggcg gggattcttc ctgcggccag
    34801 tcgcggatat aggccttgag cagcttgttc tcgaagttct ggctgtccac cacggccttg
    34861 gccacgtcgt agaagctgac cacgcccatc agcatgcgct tgtccatcac cggcatgtag
    34921 cgcgcgtggc agcccagcat catgcggcgc acctcgtcca tgtcggtctc cagcgtgcag
    34981 gtcaccggcg cgtcgtccat gctgctacgc accagggtgg tgcccacgcc gccgccgttc
    35041 ttcaccaggc gctgtatcac ctcgcggaag gtcagcatgc ccaccaggtc gccatgctcc
    35101 atgaccacca gcgagccgat gtccttctcg gccatggtct ccacagcgcg cgccagcggt
    35161 tcgtcgggat ggacggtgta gagggtgttg cctttgaggc gcaggatgtc gctgactttc
    35221 atggggtatc tccgaatggt gcggcaggcg ctgcttgccg actgcttgcg aggaaatata
    35281 gcccacaatc agacgccgac tcaccccgat ccactacttg ggagacatgg atgcccggtt
    35341 actccgaccc cggcttcgac accctggcgc tgcatgccgg cgcccagccc gaccccgcca
    35401 ccggcgcgcg tgccgtgccc atacacctga cggcctcctt cgtcttcgaa tccagcgacc
    35461 aggccgcggc actgttcaac atggagcgcc ccggccatgt ctacagccgc atcagcaacc
    35521 ccaccaacgc cgtgctggag caacgcgtgg cggccctgga gggtggcgtc ggcgccatcg
    35581 ccacggccag cggccaggcg gcgctgcacc tggccatcgc cacgctgatg ggcgcgggcg
    35641 ggcatatcgt ggccagcacg gcgctgtatg gcggctcgca gaacctgttg aactacacac
    35701 tgcgccgctt cggcatagag acaacgtttg tgcagcccgg cgacctggac ggctggcgcg
    35761 ccgccatccg ccccaacacc cggctgctgt ttggcgagac ggtgggcaac cccggcctgg
    35821 aggtgctgga catccccgcc gtggcgcaga tcgcccacga cgccggcgtg ccgctgctgg
    35881 tcgattccac cctcacctcg ccctggctca tcaagccctt cgagcatggc gccgacctgg
    35941 tctaccactc ggccaccaag ttcctctcgg gccacggcac ggtgataggc ggcgtgctgg
    36001 tcgatggcgg cagctttgac tgggaaaaat cggggcgctt tcccgagatg acccagccct
    36061 acgacggctt tcacggcatg gtgtttgccg aggaaagcac cgtgggcgcc ttcttgctgc
    36121 gcgccaggcg cgagggcctg cgcgactttg gcgcctgcat gagcccgcac accgcctggc
    36181 tcatcctgca gggcatagag accctgcctc tgcgcatgca acagcacatg cgcaacaccg
    36241 agaagctggt gcaattcctg gccgaccagc ccttcgtggc gcgcgtgggc catccgctgc
    36301 tgccatcgca ccccagccac gccctggcgc aaaagctgct gccgcgcggc gcgggctcgg
    36361 tgttcagctt cgacatcaag ggcagccgcg cccagggcaa ggccttcatt gaagcgctga
    36421 agctcttcag ccacctggcc aacgtgggcg actgccgctc gctggtgatc cacccggcca
    36481 gcaccacgca ccaccgcctg agcaacgagg cgctggccgc cgccggcata ggccagggca
    36541 cgatacgcct gtccataggc ctggaggacg ccgacgacct gatcgacgac ctgaagcgcg
    36601 cactgaaggc cgcagaaaag gcaggcggct gatgtacctg caaatcaatg gcgcgcagac
    36661 cttttgccat accggcggca agccgtttga tgccggccag cccagcgtgg tcttcatcca
    36721 cggcgtgctg tgcgaccaca gcgtctgggc cctgcaaagc cgccatctgg cgcaccacgg
    36781 ctggaacgtg ctggccgtgg atctgcccgg tcacagtcgc agcggcggcg aggcgcccgc
    36841 cagcgtggaa caggcagcgg acttcatcat cgcgctgctc gacaccctgg gcgtgcagcg
    36901 tgccgcgctg gtgggtcaca gctggggttc gctgatcgcg ctggaggcca cggcccggct
    36961 gggcacgcgt gcgagccacc tggtgctggt gggcacggcc tttcccatga aggtttcgcc
    37021 agcgctgatc gaatcgtcac tgaacgagcc cgagaaggcg ctgaagatgg tcaacatgtt
    37081 ctcgcgcagc agcctggcct cgccctcggg cgcgggcttc tgggcctacg gcgcgggcct
    37141 ggccctgggt cggcgcgtgc tgcgcagcaa cccgcgggtg aatgtgttcc accgcggctt
    37201 cgtggcctgc gacagctatt gcggtggcga gcaggcgatc gccgccattc agtgcccggt
    37261 gttgtttgcg ctgggcgcgc aggatcagat gacgcccccg aaggccgccc agggcctgat
    37321 agcgaccgcg cgcgccgccg gcaagatggt gcaggtggcg cacctgcccg tgggccacaa
    37381 ccagatgacc gagacgccgg aggagacgct gcgcgccatc ggcgatttcc tgaacgcccc
    37441 ggcctaaggc atcaggccaa cagccggcac agctcgcgca ggctggtcac cgtcgcctgg
    37501 ggcggcgtgc cctgcggcca ggcggcctgc gccgggttga gccaggcggc ctgcatgcct
    37561 gcatccagcg cacctcgggc gtcgagcagc gcatcgtcgc ccacatgcag caccgcgccg
    37621 ctggtcacgc ccgctgcctg cgccgcggcg tggaagatgc gcgcatcggg cttggccacc
    37681 cccaggcgcg cagcgctcag gctgtccttg aagtagcgcc caatgcctat gtggtgcacg
    37741 tcggcattgc cgttggacaa ggccaccacg gggtagcgcg cggacaggaa ttccagcgtc
    37801 tccaacgcat cgtcgaacag ctccacgcgc tggcgctcgg caaagaagag gtcgaaggcc
    37861 ggctcggcca gcgcgggatc gtcccccgcc tgggtcagtg ccaggcggat cgactcgcgc
    37921 cgcagcgcgc tcaagtccag ccgcaggtcg gggcgcagct cgttcatgcg cacgcggatg
    37981 gcgcgcagcg ccggggcgtc accaaacagc gccgccgtgg ccggggcatg ggtcgccagc
    38041 cagccggcca gggccgcctc ggcccgtgca atggtgggcc atacgggcca tagcgtgtcg
    38101 tccaggtcga tggtgatggc gcgtatgcgc gcggtgtcaa ggcttgttac cacaattgat
    38161 ccacagcaaa actcagggag gcggccaggg cacaatggcg ctccgtgacg cagcgcaaca
    38221 ttttctccac cctccgccag ccgggcatgg tactcgccat gctggccctg ctggcgcagc
    38281 tgtggatggt gcaggccagc gcccggcact gggccgacat ggccacccag gcgctgttca
    38341 gtgccgacat ttgctcggta cacggacagg ctggcagccc cggcccgcaa ggcgacgagc
    38401 agcccatggg cgggatgcag cattgcccgg tctgcagcgt tgccggcgcc agctttatcg
    38461 ccagcggcgg cagcacggcc acgctgccgc gcgcgccaca gggcccgccc ccgagccatc
    38521 acgacagcca gccccagcgc gagtccaagc ggctgcgccc gccggcccag gggccgccac
    38581 aggccacggc ctgagacccg ttctcttcct gtcttcgtga tgcagccagc cccgccgggg
    38641 cctggctgtg cccgggcccg cgcccgcatt ccgcaccagt ttcaaaccca tgatttccaa
    38701 gacccgcatt cttcgcggcg acggcccgct gcacggctgc gccctgctcc ctcttgccgc
    38761 cctgctggca agcctgcccc tggcggccac agcacagcag gacactgccc tgcaaaccat
    38821 caccgtcacc gccacacccg tcaacgaaac ccccgtggcc gtaagcgaag cgcggcagcg
    38881 cgaaatccgg gcagcgcgcc ccgccagcag cgacagcgcc cagctgttac gcaacctgcc
    38941 cggcgtcagc ctgtcgggcg cgggcggcgt gtccagcctg cccagcgtgc atggcctggc
    39001 cgacgaccgc ctgcgcatac aggtggacgg catggatctg atctctgcct gcggcaacca
    39061 catgaacccg ccgctgtcct acatagaccc cacgcgcgtg ggcagcgtgc gcctgtttgc
    39121 cggcatcacg ccggtgagtg tgggcggcga cagcatcggc gccaccatcc aggtggaatc
    39181 cgcgccgccg accttcgccg cgccgggtga gccggcacgc atctccggcg aggccggcgc
    39241 tttctggcgc agcaacggcg acgcgctggg agcgcatctg gcggcgtcgt atgcagccga
    39301 gcaattcagc ctgcgctacg acggctccac cgcccaggcc gacaactaca aggccgcgcg
    39361 cagcttcaag agcgccggcc cggccgccag cgacaagccc acgcaatggc tgggcgacga
    39421 cgaggtggga tcgagcagct acaagacgcg caaccacgca ttgaccatgg cctggcgcgg
    39481 caacaccgac ctggtggagc tgcgcctggg tctgcaggac attccctacc agaactaccc
    39541 caaccagcgc atggacatga cgcgcaacga cagccggcag ttcaacctgc gctaccaggg
    39601 ccagttcggc tggggccggc tgcatgcccg cgcctaccac gagaacacgc gccacgccat
    39661 gaactttggc gccgacaagc agttctggta cggccccgcg ggcaacgtac ccggcatgcc
    39721 catggacacc gagggccgca ccagcggcgt gcagctgcgc ggcgacgtgg agctgtcgga
    39781 gcgcgacacg ctgcgcgtgg gcgtgctggc gcagcgctat cggctggacg actggtggct
    39841 gccctcgggc ggcggcatgg cgccgaacac cttctggaac atccgcgacg gccagcgcga
    39901 ccgcctggag gcctttgccg aatgggaggc gcgctggagc ccgcaatggc tcacccagat
    39961 cggcctgcgc gccggccagg tgcgcatgga cagcggcgcc gtgcagggct acaacagcgg
    40021 ctacgcggcc gaggccaacg ccttcaacgc ccgcgaccgc cagcgcacgg atcacaacct
    40081 ggacttcacc gcgctggcgc gctacacgca caacgaccag gccagctacg agttcggctt
    40141 tgcgcacaag acgcgctcgc ccaacctgta cgagcgctac acctggtcca ccggcggcat
    40201 ggccatgcgc atgatcaacc tggtgggcga cggcaatggc tacgtgggca acccagacct
    40261 gcgccccgag gtggccaaca ccttgagcgc cacggccgat tggcatgaca ccacgggcga
    40321 gcgctggggc gtgaagctca cgccctatgt cacgcgcgtc aacgactaca taggcgccga
    40381 acgctgcacg ggcggcctgg gcgccatgac ctcctgcacc gcggccaacg ccggcgccac
    40441 gcagggcttt gtctacctgc gctttgccaa ccaggacgtg cgcctgcacg gcctggacct
    40501 gtcgggcttt agccggttgg gcgacagcgc cgcctggggc cgcttcacgc tgcacggcat
    40561 gctgggctat gtgcgcggca aggataggcg cacgggcgac cgcctatacg ccatcatgcc
    40621 gctgaacgcc cggctggccc tcgcccacga ccagggtggc tggagcagca tggccgagct
    40681 gctgatggtg ggcgccaaga agcatgtttc cgacctgcgc aacgaggttc ccaccgccgg
    40741 ctatgccctg ttgaacctgc gcaccagcta cgagtggcag gcctggcgcc tggatctggg
    40801 cgtggacaac gtcttcaacc gcttctacca gcatccccaa ggcggcgctt acctgggtca
    40861 gggcgccacc atggcgggca cggcagtgcc ttggggggta tcggtgcccg gcatgggccg
    40921 atccttctac ctgggggcga cggtgaagtt ctgacggcat atggcccgcg gcgcagcgca
    40981 agcgcgcggc gggcttttga cgcatcgccg cattcctccc gcgcgtgggc gaaaatcggc
    41041 catggactta caaaaagaac cggccccggg ccccgcgcat gacactgccg tcttgctctg
    41101 caacctgggc acgcccgatg cccccaccgc accggcgctg cgccgctacc tggcgcaatt
    41161 cctctccgac ccgcgcgtgg tcgagatacc gcgcgccgcc tggctgccga tactgcacgg
    41221 catcatcctg cgcacgcggc cggcgcaatc ggccgccaag tacgccagca tctggaccga
    41281 cgagggctcg cccctggcgg tatggacgca caagcaggcc gtgatgttgc gcggctggct
    41341 gggcgaggcg ggcatacagg cccaggtgct gcctgccatg cgctacggcc agccggcgat
    41401 tgccgcgcag ctgcaagccc tgcaggatgc tggcgtgcgc cgcgtcctcg tgctgccgct
    41461 gtacccccag tactccgcca ccaccaccgc cagcgtggtg gatgccgtga atgcctggac
    41521 gcgcgacgcg cgccatttcc ccgagctgcg cttcgtgaac gactaccacg accaccccgg
    41581 atacatcgcc gccctggccg ccagcgtgcg cgcccactgg cgccgcgaag gggcgcgcgg
    41641 cgacaagctg gtgctgagct ttcacggcat acccgagcgc aacgtgcgcc tgggcgaccc
    41701 ctacgccgac cagtgccgca gcaccgcgca tttgctggcg caggccctgg gcctggccga
    41761 cgacgagtgg ctgctgacct tccagtcgcg ctttggccgc gccaggtggc tggagcccta
    41821 cacccagccc acgctggagc ggctggcggc cgccggcctg gccagcgtgg acgtgctctg
    41881 cccgggcttt gccgccgact gcctggagac gctggaagaa atcaacatgg aggtgcgcga
    41941 ggccttcctg cacgccggtg gcaagcgctt tgcctacatc ccctgcctga acgacaaccg
    42001 cgagtggatc accgcgctgt ccagcatcgc ccagacccat ctggcgggct ggacagcgcc
    42061 cgccgccaag gtacagccat gacgccaccc tgcccccggc tcgcccccga gcaactgcgc
    42121 ctgcacatcg cccccgagag tctgggcttt gccgacacgc gcgcgctggt gggcgagccc
    42181 ctgccctgga taggccagca gcgcgccttc gaggcggcgc gctttggcct gggcctgcag
    42241 cagcccgact accacctgtt cgtgctcggc gaggtcggca gcggccgcgc atcgctgatg
    42301 cgccaggcca tgaaagaagc ggccgccggc cggcccgtgc cgccggatct gtgctttctg
    42361 cacgacttcg atgccacgga gcggccacgg gcactgcgcc tgcccgccgg ccaggggcgc
    42421 cagctgcgcg atcagattgc agcctggagc cgtctgctgg aggccgagat cccgaagcgc
    42481 ctggccgcac ccgatgtgaa gagcgagagc gaacgcattg tcaagcgcta ccagagcgat
    42541 gaggcgcagg ccttcggtga gctggccgag tttgcgcgcg cgcggcgctt tcgcctggtg
    42601 cgcgacgagg gccagatgct gttcacgctg ctcggccccg gcggcgagga acccctgatc
    42661 gaggccgagg cagctgcgtt gagcgcaccg cagcgcagcc ccctggagca gtccgagcag
    42721 gagctgcgcg ccgagatcgt gcgctttctc gaccgcaccc ggccgctgga gcgcgcacgc
    42781 gacgaggcgc tggcgcagct gctgcgccaa agcgccggcc cgctggtcac gcatgggctg
    42841 gacgatgtgc gccaaggcct gcgcaagcag atcaaggaca cggtcaagct cgggcgctgg
    42901 ctcgatcagg tggaagagca ggtgctggac aacctggatc tgttcgtgcc ccacgagggc
    42961 ggccagggcg aggacgcgct ggaggacgag cgccgcgagc aactggccga tctgctggcg
    43021 ctatgccagg tgaacctggc ggtggacaac accggcctgg ctggtgcgcc ggtgctggtc
    43081 gaggacaacc cgcaggtgcg caacctgttc ggccatatcc agtacgaatc cggcgaggac
    43141 gcaccgtatg cggacttttc cggcatccac gccggcggcc tgctgcgtgc gcatggcggc
    43201 tttctgatgc tgtttttgcg cgacctggcg ggcgacgcgc cgctgtggga gcggctgcgg
    43261 cgctttctgc gctgcaaccg cgtgagcata gaagaccagg ccgccggcca cggcagcggc
    43321 gcccccgtgg cgctgcagcc tgaaggggtg gacgtggagg taaagatcgt gctcgtcggc
    43381 tcggccgccg agtactacct gctgcaggac ggtgacgccg atctggcgcg gcgttttcgc
    43441 gtgaaggtgg actttgccga gcagtttcgc gccagcgacg cgacgcacca tgccagctgc
    43501 gtattcctcg cgcgctcctg cgcgcgccgc ggcctgccgc attgcacggc cgcggcggcg
    43561 gcgcgcctgt tggaattcac gcaccgtgag gccgacgacc aggcgcgcca gagcgccagc
    43621 tttgccagcc tggaggcgct gctggtggag agctgcgccc aggcacgcgc acgcgcggcg
    43681 cagctgaccg atgcgcgcga cgtggatgcg gccctggccg cccggcgtac gcgccacaac
    43741 gcgcccgagg aggcgctgca cgaagcgatt gccgagggcg agctgctcat cacgctggaa
    43801 ggccagcgcg tgggcctgct caacgcgctc acgcagatcg acatgggcga ctaccgcttc
    43861 ggctttccgg tgcgcatcac cgcacgcacg cacgccgggc gcgagggcgt ggtgaacatc
    43921 gagcgcgagg tggagatgtc cggccccatc catgacaagg gcgtgctgat cctgcaaagc
    43981 tatttgagcg cgctgttcgg ccacctggcg ccgctggcgc tgaacgcctc catcgtcttc
    44041 gagcaggagt acagcggcat cgagggcgac tcggcctcct gcgccgagct gtatgcgctg
    44101 ctgtcctcgc tgtcgggcct ggcgctgcgc caggacatcg ccgtgaccgg cgctctcaac
    44161 cagcatggcg aggtgctacc cgtgggcggg ctgaatgaaa agatagaggg ctggtatcgc
    44221 gtctgcgcgc aggccggcct gacgggcacg cagggcgtgc tgatcccggc gcgcaaccag
    44281 cgccacctga tgctctcgcc ccaggtggtg caggccgtgg ccgacgggca gttccatgtg
    44341 catgccgctc agcatgtcag cgaaggcgtg gcgctgctca ccggccacga cgcgggcctg
    44401 gcacccggcg aatgcctgcc gggcagcgtg catgcgcgcg ccgaggccac gctgctggcc
    44461 taccgccgcg cctggcaggc gatggcgctg gggcgccgct tgccccaacg cgggcgttga
    44521 tcaggggtgc cggatcagcg ctccatccac cactggatga cgatcttcgc caggtagccg
    44581 atgaagccca cgcccaggcc gagaaagatg acgaaggtgc caaacctgcc ggccttggac
    44641 tcgcgcgcca gctgcaggat gatgaacacc atgaacagga tgaaggcccc caccccgtag
    44701 gtgaggaaga aatgggctat ttgctgttct gtgtacccaa aaatcacaat aacttcctac
    44761 caacaatccc agaagcctca aggcgcaggc gcgtcgagcg gcgcaaacgc cgaggcatcg
    44821 accagctcgc gatacgcatg ccagctggcg tggcccagca gcggcatcac gaccaccagc
    44881 ccgatcagca gcgtgcccag gcccagcagg gtcagcacca cgatcaaggc cgcccacagc
    44941 gccatgggca ccgggttggc cagcaccacg gcccagctgg tcagcacggc ctggcgcacg
    45001 gtgatgcgcc tgtccagcag caacggcatg gccaccacgc tggacgcaaa aatgggcgcg
    45061 gccagaaaac tgcccagcgc cagccagatg gcaaacagcg ccccctcgcg ggccagcacc
    45121 acatggcgca gaaaatccat gggggtatcg acgcgcaccg gcgacagcgt gtagatcagc
    45181 gacgccgaga tcgccaccca ccccgtggcc gccagcgaca gcagcaggcc aaagcgcacc
    45241 aggctccaat agccctggtg ctgcgcctgc cccgcgtggc tgcgctgcca gctcgcccag
    45301 gtctgccaca ccaggcccag gcccacgcgc tcgccgcgct ccagggcacg gctgatggcg
    45361 tacaggctgg tggccaacat aggcccgacg atcaaaaaac ccgacagggt gcccgccacg
    45421 aaccagaaac ggttgtaccc cagcgacagg atggcggcgc ccagcaaggc cagcaccagc
    45481 ccatgggcca ggctcaacag cggcgcgcgc gcaatgtcgc gcagcgccag cgtcagccac
    45541 gccagcggcc tcagcaggcc tatggtgcgc acctggggca ggacaagcgg cgaagcagga
    45601 cggtttcgga ggctgtgggg catgggacgc ggctgggcaa tgaccggcgc agtctactgc
    45661 acccgggcag ccacccatgg cacatgtggc gcagggagcg cttatgaata attggcataa
    45721 gaacaagtca attctgtagt ttgcaagcag aaggacagcc cacacaatca cgcccaatgt
    45781 tgaaccctgc cccaggactg gccatgaaga aactgcttgc cctcgccgcc ctcgccacgg
    45841 cctttcacac cgcctgggcc gcgcaacccc tgctcacccc cgccgagctc agcgcccagc
    45901 aggcgcaggc gagcgccccg cgcgtgatcg acatccgcga cccggccagc tacagcgccc
    45961 agcacatccc cggcgccatc aacgccccct atggcaaatg gcgcggccct gccagcaacc
    46021 ccggcgagct gctggacgca cccaagctca ccgagctggc gcagtcgctg ggcctgacgg
    46081 ccagcacgca tgccgtcatc gtctccaccg gcgccgacgc caccgacttt ggcgccgccg
    46141 cgcgcgtgta ctggacgctg aagctgctgg gcctcaagga gctgtccatc ctcaacggcg
    46201 gcatgaaggc ctgggccgat gccaagctgg cgcaagacca ggccgtgccc caggtggcgc
    46261 gcagccagta ccagccgcag atcgacacca gcctgctggc cacacgcgag gatgtgggcc
    46321 agcacatcaa gctgagcaac gccgcgctgg tcgattcgcg ccctgacgca ttctttttgg
    46381 gcaagacacg cgcgcccgcc gccaagctgg ccggcaccct gcccggcgcc cagcagctcg
    46441 acttcaacca gtggttcctg cccggcactg cgaccttcgt ggacaagagc gcggcgcaga
    46501 agattgccgc ccaggtcaag cgcgaacagg gccaggacat ggtggccttc tgcaacaccg
    46561 gtcactgggc cgcgaccgac tggtttggcc tgtccgaggt cgccggcctg cccggcgtca
    46621 agctctacgc cggctccatg gtggactgga cccagtccaa ggacacgccg ctgatggcca
    46681 accagcccag ccgcttcgag gccctggcct atgacgccaa acagtggtgg gaacgcaagt
    46741 ccaagtaatt ccgtttctaa atcacccgtg tcgttgttac ttcgccttgt cgtgctacag
    46801 cactgcctgc ggcttcgcgc ctagacacga atgatttgga aacggaataa ggccgcaggc
    46861 caatcaccga tttatagtaa aaacggctgc cagcgcttga tggaaaagcg ctggcagctc
    46921 acttttttat agaactcttg cttcccatga aacgactccc cctcgcggcc gtgctggccg
    46981 ttttcactgg ctggctgctg tggagcgtgt cgacgcgcca ggcggccctg tttgccgtgg
    47041 ggctggggct gggcgcggtg ctggcgggcc agcgctttgg cttcaccacc ggctggcgca
    47101 tgctgatcga gcagcgtgac gccagcggcg tcatgggcca actgctgctg ttggcactgg
    47161 ccgcggccct ggccatgccg ctgctgggcc attaccccga gctcacggcc gcactcggcc
    47221 cgcccagcgt cagcctgctg gtgggcgcgt ttgtcttcgg cctgtgcatg cagattgccg
    47281 acggctgcgg cagcggcacg ctgtacaagg ccgggttggg cattcccatg aacgctgcca
    47341 tcctgccgct gtttgcgctg ggcagctttc tgggctcgct gcacctgggt ttctggctgg
    47401 atctggggcg cgtgcagccc gtgggcctgg tcaaccagtt cggctgggtg caggcgctgc
    47461 tcatcaccct ggccgctctg gccctggcgg ccgtggccgt gcgctggtac gtgggccgcg
    47521 ccagccaggc cgcgggccag gcgcccaagc cgctgctggt gcgcaagtgg atgctgggcg
    47581 cggtgctgct ggcgttgctg gccacggcaa acctgctgat tgccggccag ccctggggcg
    47641 tggtctacgg ctttggcctg tgggcggcaa aactggccca ggcgacgggt gcggcggatc
    47701 tggcgggcaa ctggttctgg agccagggcg gcaacgccgc acgcctgcag gagacggtgc
    47761 tgatggacgt gaccagcatc accaacatcg gcatcctggg cggcgcgctg tgggtgtcgg
    47821 cgggcaaggc caccacggcc aagcccttga acggcacgca atgggccgtg gctctggtgg
    47881 ccgggctggc gctgggctac agctcgcgcc tggcctttgg ctgcaacgtg ggcgccatgc
    47941 tctcgggcat ctccacgggc agcatccacg gctggatctg ggtgccgctg gcctttgccg
    48001 gcaccatctt cggcctgcgc atccgccgcc atttcggctt ttgaagtacc cgcatcacgc
    48061 catgacacag cacaccacct tgcgcctggt cttctgggcc attcttgtcg ccttcctggc
    48121 ctgggactgg gccggctcca agcccgtgga tctgcgcctg gagccgccgc tgatcgccgc
    48181 cggatcgggc caggcggcga cgggcgggca ttgctccatg ccggattgac ccgcccctgc
    48241 cccgcaaaca ggaaggcctc cgtacggagg ccttctgcgt tgccggcggg cgttcggtga
    48301 cgcctgccct gactcccgat cagcgcggca cggtgatgga catgccgctg aagcctatct
    48361 gatccgccgc acccggcgcc atggcaaatg gcggcacgtt ggcggcggtg atggccggcg
    48421 ccgccgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcgccatggc
    48541 aaatggcggc acgttggcgg cggtgatggc cggcgccgcc gccgcccccg ccgtaccacc
    48601 gcgtggcgtg gtgcgcacca cctggctggc gggcagcgca ctaccgctct tcagatgcgc
    48661 ccacatcaca tccatggcct ggttgaagta agggtgcagc ggcacgaagc gcgtgtcaaa
    48721 gccgctaaag ggcaggaagg catcgaaatg ctggccattg acgacctcga tgtagcgcag
    48781 cttgctggcc gcgccttcga tcgtgcggtt cagcgccgtg taggcgcgcg cgttgtggtt
    48841 caccggcacc agcgcatcgc tgcggccggc aacgatgatg gtcggcttgc cgcgcaggtt
    48901 ggcgctgtgc agcacctcgg caatgccggc acgcacggcg tcgctttgcg ccttgcttgg
    48961 ctggctgctc gccgtgagcg cggcgcccgt ggccaggtcc ttgccactga ccagcgcgtg
    49021 ctggcacagg gcgttgtcca ggccaaagtc ggccacaccc gtggagggtg acaccgccca
    49081 gggccaggac ttggccccgc ccttggagtc gttgtagatc accgtggccg gcgtgccatt
    49141 ggccgtgccg ttggccgtgg caaagctgct ggccagcgcc gcgggggcgg ccgccaccac
    49201 gtcgcccgtg gcattggccg cggcgaagct ggcgccgcac agattggcat cgacaccaaa
    49261 gcggccatag gccatggtgt acatggccga gaggatgggg ccgttgccca acgcgtaatg
    49321 ggcgttgtgc atgctgtcgt tgtcgctgct ccagccgtag gcgcgcagcc tggccagggc
    49381 gtcggcggca cgctcggccg tcgtcgcgcc agtcaccagg cccttggccg ccaggccatc
    49441 gcagcgcgcc gtggcgcggg ccgtcatggc ggtcagggcg atgtagttgt agatcgaggt
    49501 ctcggccatc gccgcatcgg ccgccagcgc cgcgcagggc tggtagatgt tgccgtaggt
    49561 ggtgtagtcg gccagggtct tgccgtagcc cgccacggcc gcgccgccga actggatgcc
    49621 gtagcccgcc gtggtgggca tctcggtcac cggctcggag gccaccacgc cgtcgatcag
    49681 gccatcgccg tcctgctcgg ccgcgcgcag cgaggccgcg ccaccattgg aggccgagcc
    49741 tgctatcacc agcgtgttgc ccgcagtgaa cggcacgcgg ttgtcgggcg tgccgtagcg
    49801 cgcgttcaac acgtacagcg catagcggcc cgcggccagc gtgtccaggc cccagtcctt
    49861 ctcagggttc tgctgtgaat gcgcgtgctt gatggcaatg cgattgggca gaacggcgtt
    49921 gtaggcagcg cgcgcggcgt cgctcaggtt cgcggcaaag aagctcagcg ccccggccac
    49981 ggtgcgcgtg gcgcgcgtgc cgtcgatcct gttgacggtg tcgtcactca ggttgtgcag
    50041 gcccactccc ttgccggcgt cggtcagcgc cacggcacag cctttcttca ggccccattc
    50101 gcccgccgtg ccaatggcgc catacacccc tcgcgagccc gacgaggcgc ccagcacgat
    50161 gcaggggttg ctggcgctga agctgtcggg cacctgcacc gcgatcacgg tctgcttgcg
    50221 ccccgtgcca tcgtcgagca cggcgacata ttcgcggccg gggatcaggc cctcattgct
    50281 ggtcacctgg ccgtcgaccg tgacattggg gccgtacagc aggccataac cgcccccggc
    50341 cgacgggtcg agaatgccgc ggtagttgga atacagagca ttgcgccgca gctcggccgc
    50401 cgtgggattc gccgggtcgg cataggccgg cgcggcggcg gcgcccaggc ccgtcctgcc
    50461 catgccgccc gtgagcagat cctgcgtggc cgccgtgtcg cccttgccca ccgtggtggc
    50521 cgggtaggcc gtggcgctga tctccttgat gcccttgggc agctcattgg atggccccga
    50581 gccaccgcag gccgccagcg tggcggcgga cagggccgtc atgacccagc gcatcgtcgt
    50641 tccctcattt cctcacatgt tgtctcctgt ggttgtggtg atgcatgcac gagaaaacgc
    50701 acgataccgg gaaacaacca gccatccggc gcgagaaaac ccgaggagcg ggccgtcggg
    50761 ccacggcccg atgcgcgcgc tcagctcaca tcgtccacgt tttggcgcgc acgcgccagg
    50821 ttgcgctcgg cccagcggcg cagcaggcgc ggcaggatga gcacagccag cacgatgatg
    50881 accagggtca gcgacatggg gcgctgcagg aagatccacc agctgccctc gccgatggag
    50941 acggcgttgc gcatctgcgc ctcggccagc ggccccagga tcatgcccac caccaccggc
    51001 gcggtaggga agtcaaagcg ccgcatgagc acgcccagca ggccgatggc gtagagcagg
    51061 aacaggtcga aggtgctctg gcgcatgccg taggcgccca cggtggcgaa gatcaggatg
    51121 ccggcataca gctgcgggcg cgggatcttc aagagcttca cccacaggcc caccatgggc
    51181 aggttcagca ccagcagcat gaggttgccg atgtacagcg acgcaatcag cgcccacacc
    51241 agcgccgccg acgaggaaaa cagctgcggc cccgggttca gcccgtagtt ctggaaggca
    51301 cccagcagca cggccgtggt gttgctcgtc ggtatgccca gcgtcaggag cgggatcagc
    51361 gccgccgtga ccgtggcgtt gttcgccgcc tcggggccgg ccacgccctc gatggcgccc
    51421 tgggtgccga actccgcctt gtcagcaccc ctggcgagct tcttctcggt ggcgtagctc
    51481 agaaaggtcg ggatctcggt gccgccagcc ggtatgcagc caaacggtgt gccaatcacg
    51541 gtgccgcgca gccaggcggg ccaggaacgc ttccagtcgc gcgcggtcat gtgcacgcgc
    51601 gtcagcttgt tctgcgactc ctccaccttg ccctcgaaga gcgcggcgta cagcacctcg
    51661 gccacggcaa acaggcccac tgcgacgagc acgatgtcga tgccgtcgag cagctccatc
    51721 ttgccgccgg tgtagcgcgc cgcgcccgag atctggtcca tgcccacgca gcccagcgcc
    51781 aggccgatga acagcgccgt catgccgcgc aggctgctct ggccgagcac ggcgctgacc
    51841 gtggtgaagg ccagcaccat cagcatgaag tattccggcg ggccgagttt gacggcgaat
    51901 tccgccaccg agggcgcgaa cagcgtcacc accaccgtgg ccacggtgcc ggcaaaaaac
    51961 gagcctatgg ccgccgtcgc cagcgccgcg ccggcgcggc cgctcttggc catcttgttg
    52021 ccctccatgg ccgtgaccat gctggccgtc tcgcccggag tgttcagcaa tatggaggtg
    52081 gtggagccgc catacatcgc gccgtagtag atgccggcga agaaaatcat cgatgccgtc
    52141 atctccacct tggcggtgat gggcagcagc atggccacgg ccacagccgg gcctatgccc
    52201 ggcagcacgc ccacggccgt acccagggcg cagcccacca gggcccacag caggttggcc
    52261 ggcgtgatcg ccgtggcgaa gcccgccatc aatgcgtcaa aaatctccat tacagccacc
    52321 ctgtattggt aagccccggc aggttgatgg ccaggaattg cgtgaacagc cagaacaccg
    52381 gcgcggcgat cgccaggccg gtgaacacat ctaccgccag cgcgcgtggc gcaagcaggc
    52441 cctgctgccc attggcgcgg cgcaggccct gcacggccag cgcatagcac agcgtgcagc
    52501 ccaggatgaa ccccagggtt tcgatgagcg cggcattcaa gagcagcccg gcggtaaccc
    52561 aggcaaaggg cgccagcgcc gcgcgcggct cgccaccccc atcggcggcc tggcggtagc
    52621 cgccggtgag cgcctcgcgc accagcagca cgccgcacag ggccagcacc gcggcgcaca
    52681 gccagggaag aaagttgggc cccacgccgc catagccggc atcgcccggg atctgcaacg
    52741 cgcccagggc catgcccgcg gccaccagca ccacggccgc gccgacggcg gcctgccagc
    52801 gcggggacgg gatgctgccc gcctcggtgg gcggggcgag aaggtcagaa gaaactggct
    52861 caaccatggc ggcggtctcc gcagaaataa tgaaaaaaca gcctctagcg ccctgccgca
    52921 gggcgccaag agctatcaat aagatagtaa caaccggacg acaaggttct gccggccggc
    52981 gttgcgcacc caggttcagg ccatgctcaa accatgccgg acttgaccat ggtggcgcgc
    53041 aggctggcga actcgctgtc gacaaagttg ccgaattctt cgccggccag ccaagcgggc
    53101 gtccagccgt tcttctccag cgcctcggcc caggccttgg acttgacggc cttgtccacc
    53161 atggcaatca gggccttgcg ctgctcggcg ttgatgcccg gcgcgccata cacgccgcgc
    53221 cagttgccaa tttccacgtt gatgccctgc tctttcagcg tgggaacatt gggcagatcc
    53281 ttcaggcgct tcggggatgt gacgccaatg gcgcgcatct tgcctgcgtt gatgtactca
    53341 gcaaactcgc tgtagccgct gccgcctatg gtgacgttgc cgcccaggat ggcggccgtg
    53401 gcctcgccac ccccgcgaaa agccacgtag ttgatcttgg ccgggtccac gcccacctcg
    53461 cgcgcgatca tggccgcggc gatgtgctcg gtggcgccgc gcgatccgcc accccatttc
    53521 acgctgcccg ggtccttctt gagctgggcc accacctcgg ccatggtctt gaacggcgag
    53581 ttggcgggca gcacgaagac gttgtactcg ctcgtcaggc gcgcaatcgg cgtggcctgg
    53641 cccaggttca ccggcggctt gccggtgatg atgccgccga ccatcaccgc gcccatgacc
    53701 atcaaggcat gcggatcgcc cttgctgccg ttgatgaact gcgccaggcc gatggcgccg
    53761 gccgccccgc ccttgttctc ataggtgacg ctggaggcca cgcccgcgtc ctgcagcgcc
    53821 ttgcccaggg cgcggccggt ggtgtcccag ccgccgccgg gattggccgg caacatcatc
    53881 ttcatggcca cggccgcgcg cgcgggcagc ggcaggctgc ccaccgccgc cagcgccgcg
    53941 agggatttca gaaaggtgtc acgacgcatg cctgtctcct tgtttatcgg ttgcaacaat
    54001 gaacgcggcc gatgatgcgt gggcaagctg tcaaacggct gtcctcggca ctagggatta
    54061 cgcgtacacc cgctgccctg caggccacgc caaacggttc taatcgggcc atgcaactgc
    54121 ttttggtcga agacgacgcc accatgcaag ccacgctgca gcgctcgctg tcgcgccgtg
    54181 gcatggccgt gaccgccctc gccgacggcc gcgcggcgct ggccgaatgg cgcgcccacc
    54241 cgcccgacgc cgtggtgttg gacttgaccc tgcccggcct ggatggcctg caggtgcttg
    54301 cccaggcccg cgccgccggc ctgcgcacgc cggtgctgct gctgaccgcg cgcggcaccg
    54361 tgggcgaccg cgtgctgggc ctgaacgccg gcgctgacga ctacctgccc aagcccttcg
    54421 acctggacga gctggaggcg cgcctgcgcg ccctgctgcg ccgcagcgcc gacggctccc
    54481 agaccccaac gccccccgaa ggcccggtgg tgggcgcgct gcgctatgac aaggatagtg
    54541 gcgtgattta cctgcaggat gcgcccatgg acctcacgcc gcgcgagcag gcactgctgc
    54601 gcgccctgct ggcccaaccg gggcgggcct tgccgaagga acggctgtac gagctggtct
    54661 ttcccggcca gagcgaggtg cagtacgagg ccatcgaggt cgtggtctat cgcctgcgca
    54721 agaagctcac cggcaccggg gccacgctga tgacgctgcg cggcctgggc tatctgctga
    54781 aaaccgaaac gtgacccggc aaagatcgct gcgccgccag ctgctgctgg gcatcctgct
    54841 gcccacgctg ctgcttatcg gcgtgaacac ctacagcctt tatcaccagg cgctggcggc
    54901 gctgaacacg gcctacgacc gcacgctgct ggcctcggcc aagagcatca gcgagcagct
    54961 ggacgtgcgc ggctacgacg acgcggccga gctgcgcgcc accgtgccct actcggccct
    55021 ggaggccttc gaggccgaca accaaagccg catgttctac cgcgtgtcca ccttgcaggg
    55081 cgggctggtc tcgggcttcg ccgagctgcc cttctggcag ggcagcattg cccagcgccc
    55141 tccctacgcg gcgctggtgg acttctacaa cgcccgcttc cgcgaccgac cggtgcgcgt
    55201 ggcggcgctc ttgcagcccg tggccggccc ccaggggcgc accatggccg tgatccaggt
    55261 ggccgagacg ctggagctgc gccatgccgc ggcgctgcaa atcctgtgga acacgctggt
    55321 gcgccaggcc ctgctgctgg ccatgatcgc tgccatcgtg gtgctggtgg tgcagcgcgc
    55381 cacgctgccg gtgcgccgcc tgagcagcga actgcagggc cgcggcgcgg acgacctgtc
    55441 gcccattgcc gcccccgcgg ccccacgcga gctgcagccg ctgatagacg ccacgaacgc
    55501 ggtgatgcag cgcctgtcgc gcctgctggc gcatcagaag cgctttgtgc gtgacgcctc
    55561 gcaccagctg cgcacgccgc tggcggtgct caagacccag gtgcagtcgg ccctgcgcgg
    55621 cgacctgccg ccgacacagg cgctgcagga aatcggcgac accgtggagc gcgccacgca
    55681 gctggccaac cagatgctgg ccctggccaa ggtggagcag ctgcgccagc agggagagcc
    55741 gcccgtgacg cgcctggacg aggtgctgcg cgaggtggct ctggatctgt cgccgctgat
    55801 cgcgcaggcc aacctggact tcggtatcag caccgacacc gcacccatcc aggcccacgc
    55861 ctggatgctg cgcgagctgt gccgcaacct gctgcacaac gccatccgcc atgcgccgcc
    55921 cacgaccgag ctgacagtcg cgctgcacac gcagggcgcc caggcgctgc tgaccattgc
    55981 cgacgcgggc cccggcgtag acgatgaact ggccgcgcgc ctgttcgagc ccttttcggc
    56041 cggcgacatg cgccatagct cgggcctggg cctggccatc tgccaggaga tcgtgcaggc
    56101 cctgggcggc aacatcgccc tgggcaaccg ccgccagggc ggcgccatcc tggggctgga
    56161 tgcggtggtg cgcctgccgc tggcccggcc ccgagaggcc gcgcctgtga aaaaatcacg
    56221 gccatgacga ccccacccga ttccatgcgc ctggacaaat ggctgtggtg cgcgcgcttc
    56281 tacaagaccc gcagcctggc cgtcgaagag ataggcaagg gccgcgtcac cgtcaacggc
    56341 caggcggcca aggcagcgcg cgagctgcgc gtgggcgaca acgtggccct gcgccagggc
    56401 cccgtggcgc gcaccgtcgt catacgcgcc ttgagcaaca tgcgcggccc cgcacccgta
    56461 gcccagcagc tgtacgaaga aacacccgag agcatcgccg cgcgcgccca ggccgccgag
    56521 gcccggcgcc tggcccccga accggccacc gccctgcgcg aaggccgccc gaccaaacgc
    56581 gatcggcgcg acatggacga tctgcgccac ggctggggcg aacgctggag cgcgtcgctc
    56641 gacgattgat gcggtgccgt gcgcagagca tggcgcggcc cacccccacg gcgctgcaca
    56701 atgggcggct ttcccgcccg gcctcaccca tgatctacaa cgccctcaag ctcgcccatg
    56761 tgctgtccat catcgtctgg ataggcggca tggtcttcgc ccattttttc ctgcgccccg
    56821 ccgcgcaggc gctggagccg gcccagcgcg taacactgat gcacggcgtg ctgcaacgct
    56881 tcctgggcgc cgtggccatc gccatcgtcg tggtgctgac cagtggcctg ggcatgattg
    56941 gggccgtgag catgggttcg ggcggtgcat tctccatgcc cctcaagtgg accatcatgg
    57001 ccacgctggg cctggtgatg atggccatct tcgcctacat ctacttcgcc ctgctcgata
    57061 ggctggagca ggccgtcacc gcgtccgact ggagcgccgg cggcctggcc ctggcccgca
    57121 tacgcagctg ggtcggcgtg aacctggtga tcggcgtggt gatcgtggtg cagacgctgc
    57181 tgatgtaggg ccgaggctgc ccaactgacc gccggctcca cctgtgcgtg aaagtggctg
    57241 caatcgcgcc cacgatgtcg tgagccgaag cagccccgac ttctgccctc agcgccggcg
    57301 cagcacgtgg atgaactctg tgcccacggt ctgctgctcc accagctcgt tgcccgtctg
    57361 ccgggcaaag gcctgaaagt cgcgcaccga accgctgtcg gtggccacca cgcgcagcag
    57421 ctggccgctc tccatgtcgg ccagggcctt cttggccttg agtatgggca gggggcaatt
    57481 caggccccgg gtgtcgattt ctttatggat gtccattcct gtcagtcctt tggcgcctcg
    57541 tccagtccgc ggcggcgctc tgcattttcc tcggccgtga agaacaccgg ctcatgcccg
    57601 cgtgagcgca gccagtcggc cagcgcgtag ccggtgccgg cgggccagca cttgagttcg
    57661 tccagctcgt agaagcggta gtccaccagc tccggcgaca gccgcacctg cccctgggcc
    57721 tgcacatggt aggcgatgat gacctggttc atgcgcagaa actcgtatgc gcccaccagg
    57781 cgcacagcgc gcgtgtccag gttggtttcc tccttcacct cgcgcgcaat gccttcctcg
    57841 ggcgactcac cggcctccat gaagccggtg atcaaggcaa acatcttggc cggccacagc
    57901 gcattgcgcg ccagcaatat cctgccatcc acttcgacga tggccgccag cacgggcgtg
    57961 ggattgttcc agtgcgtcca gccacaggcc gggcagcgca ggcgctcctt ctcgccgctg
    58021 tcctccatgg tgacaatggg cgccagcgcg gtcgcgcaat ggctgcaaaa gcgggtctgg
    58081 tattgcatgg tgcggcgctc gctcaggcgg gaaagacgcc ggtggacagg taacgatcgc
    58141 cccggtcgca aaccacgaag acaatggtgg cgttctgctc ctgcgccgcg atctgctgcg
    58201 ccacccagca ggcgccggcg gcggagatac cgccaaagat gccctcctcg cgcgccaggc
    58261 gccgcgccat gtcctcggca tcgtcctggc tcaccagcac cagttcatcc accaggttgg
    58321 gctggtagat cttgggcagg tattgctcgg gccacttgcg tatgccggga atgcgcgagc
    58381 cctcctgcgg ctgggcacca atgatgcgca caccggggtt cttctcgcgc aggaagcgcg
    58441 ccacgccggt gatggtgccg gtggtgccca tggcgctcac gaaatgcgtg atgcgcccct
    58501 cggtctgctc ccacagctcg gggccggtgg tttcgtagtg gatgcgtggg ttgtcggcgt
    58561 tggcaaactg atccagcacc acccccttgc cctgcaccac catctggtcg gccaggtcgc
    58621 gtgcgtattc catgccgccg ctcttgggcg tgaggatgag ctcggcgcca aaggccttca
    58681 tggtctgcgc ccgctccacc gacagatcct cgggcatgat cagcaccatg cggtagcccc
    58741 ggatggcagc ggccatggcc agcgcaatgc cggtgtttcc cgacgtggcc tcgatcagcg
    58801 tatcgccggg cttgatctca ccgcgctcct cggcgcgccg gatcatggcc agggccggcc
    58861 tgtccttgac cgagcccgcg ggattgttgc cctccagctt gcccagaatg acattgccgc
    58921 gcgccgcatt gtcctgtgcc gctatgcgct gcaaggcgac cagcggtgtg cgcccgatgg
    58981 cgtcttcaat ggtgggatag gtcttcatac gcccttactg tgccataatt tacgacttcg
    59041 cttttagcgc ctgcccgggt ggtgaaattg gtagacgcag gggactcaaa a
//
LOCUS       DAOMTQ010000004        54535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_23_length_54535_cov_24.597008, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000004 DAOMTQ010000000
VERSION     DAOMTQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 54535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54535
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_54535_cov_24.597008"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PK518_00960"
     CDS             complement(<1..955)
                     /locus_tag="PK518_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRM47508.1"
                     /translation="MQGDLSRETFDARKHYTAVRLQQGRVLTDADFNEQGDITRQRLE
                     HLARDVIGASGGPAEGAGFALAGGMAALAVHAQDANSIWIAGQDGVLLVSSNGGGAWT
                     VANTGSTRHLRALARSGSTGWAVGDGGTILRTSNSGSSWTAQASGTLQALRGVACTDA
                     QHAWAVGDGGLALATVDGGVTWTARATGVARLHAVAFTSAQNGLAVGQGGAIVRSADG
                     GQTWAKVGSGSQATLRAVVLNGAQALAAGDGGTLLVSGDGGATWSTATSGSTARLRTL
                     RMRDANTGWAAGDGGTLLATGNGGASWTAQPVAGSPQLAGLS"
     gene            complement(981..3296)
                     /locus_tag="PK518_00965"
     CDS             complement(981..3296)
                     /locus_tag="PK518_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47509.1"
                     /translation="MSLLTPERLYQLLPAIHRLRDAEQGQPLRALLAVIEEELTALEL
                     DAARLYDNWFIETCDEWTVPYIGDLVGARAIRPVPSAGVSMRAWVANTLAYRRRKGTA
                     LVLEQLARDVTGWPATAVEFFQRLATTQHMNHVRRAPAATAGVRDAARAELAQAHAGA
                     FDPYAHTLEVRNADTRGGRHNIPNIGIFLWRLRAQPLGQGVPGEADADFISARDSGAG
                     WWAMHPAGVDAPLFNRPRTLTGGALTQAAQAAREDNVSAPLRSLALHAELERLRAGMA
                     EPPPVFMTAQQPGLRVFAQLAGESLPVEIPRERLWICEIPNAVTLPVPPRAAALDVRR
                     GRIAFPAAANVQQVWLQAAHGSVADMGGGPYDRGDALRAASASLSEGTATNADQGGFL
                     APTVWQAGVTHLLALGSTGQLFATLRDAVTAWNALPAGRTGVIVVMDSLSETDAAPLT
                     INLAEGSRLMIVSGQWPLVPVPGALPGTLERLPGRFEARDTRAHWQGSLRVVGQAAPA
                     SPNPGALFINGLLLQGPLSLSGPLGQVELAHCTMLPSTTHITVAGDSPRLALRVLHSL
                     TAPIAAAGPVTSITLADSIAGLLDEGAAPQPALNAPDAPLDLQRTTVLGSVSGLTLTA
                     SDCIFDAPVTAVRRQTGCVRFCYVPPASQVPRRYRCQPQLESDTRIAALRAKAAAAGL
                     PPPTGADEAALRAEVEATVRPLFSARRYGDPALGQLDLRCAVQIRTGAESGAEMGAYE
                     QLRQPQREANLRDALDEYLRLGLTAGLHYAN"
     gene            complement(3293..6208)
                     /locus_tag="PK518_00970"
     CDS             complement(3293..6208)
                     /locus_tag="PK518_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47510.1"
                     /translation="MSDDHLSGCHCCEGQQPRPAIYNDPGLPALAWRLDTQPGFYQRM
                     LARLPLWRDPEAGAGAPRPLAHLATREPVDATVALVDAAACAADVLSFYQERIANEGF
                     LRTATERRSVLELARAVGYELRPGVAAGVHLVFTVEDAPGAPGLCTIAAGSPIQSVPP
                     QGKLPQVFETSAELLARAEWNALRPRLTRPADMALLNVSGGARRLVLLLPQGSLPAGT
                     AGLHQGLASGSLYRLDPGLPVDASVDALEVGRIYLGANANRIQAGELLLFVGKNGANL
                     LHLVLRAREVEEEKTLERVRIDLDPLPDPVAPAAPPMLQWAIPVMYAISGPYAQAQVQ
                     SVPFTSGSISTTFSGKAWQEGELQAMIAIQGWSGQQLVQAITASAQPATKPVAPEAGA
                     FSFGARLGFFGHNAPQWNSLPASNTKGGATYYPANWDSTNPSIWTDSQSGAITPQHTY
                     LERPVPALAAGSWMVFDSPGVKASTYLLYDSRECARADYGLSGRAMALRMAGSDGKPL
                     SAPPSEAFKFRDTTAHVASQRQPLVDLPIDAPVAAGSQRLELSTMVLGLTPGKLLAFV
                     GARSDIPGVDAAEIAVLDDVVHANGRSTLVLRGKSGLQFSYQREGLRIHANVVAATHG
                     EGVQEVLGNGDASQPFQQFTLRRPPTTHLSAASSSGAQSTLALRVNGLLWSERPSLYG
                     AGPNEHVFATRIDNDARMTLLFGDGRQGARLPTGQMNVRARYRTGLGADGEVAAASLT
                     MPRAMPLGLRGVNNPLPAGGAQDPEKLADARRNAPLTLLAFERVVSLRDYQDYARAFP
                     GIGKARADLVSVDASTRVLLSVTGATGGTADAQVLDNLRLAITDQSDPAQAFTLQAAA
                     LRYFRCQASVVVDGRYQATAVLADCLARLLEAYGFDARELAQPVTAAALLTLLQQVSG
                     VVAVDLSVLQPYGQGASPDAVQEVLPALGARWVAGAMQPAELLLINPAAVQLLEAMP"
     gene            complement(6205..8688)
                     /locus_tag="PK518_00975"
     CDS             complement(6205..8688)
                     /locus_tag="PK518_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HRM47511.1"
                     /translation="MSTQQYLCKNPRRIAALRAAAQELPPRWFNGIEYLEVIHGEPRL
                     VLHFVHDLSQTPVAPLTAGNVEVRGGQRVRDPHVVGVSASGKQLTVELDDSGDFSRYQ
                     LRLVASAGADAPPDGIDPALAQISFGFKVDCPSDFDCRQTRDCPPETQAVPDIDYLAR
                     DYPTFRRLVLDRLSVLMPDWRERNPADLLVTLSEALAYRADEIAYFQDAVATEAYLGT
                     ARQRVSVKRHTRLLDYQLAEGGNARAWVAFEVDATADGQQLAGCDPDTGLDGTLLLTQ
                     SAGTAKRLPSRARAAELVAGGAQPFELLTPVTLYSAHNDLRFYTWSDEACCLPKGATR
                     AFVRDAVAQRLRLRAGDLLLLEARAGTSNGLPQDADPTQRHVVRLTRVDPEADAARQP
                     APTPRRDPVTLQPIVELQWAEEDALPFDLCLSKVIGGSLVQDMAGACANLALADHGMS
                     PSATQKLLPVPGGRVPRLRPEHGSQLPLTRQAMVRDALGRLTLVDATAPAAAAMQTDP
                     AGQRAAMRVTSDGDGRLWSVQPDLLGSNGQAADYVVETDNDASVLLRFGDGVNGRSPG
                     ATEGLSLRMRGGNGSNGNVGSNAIAHVLCGFDGITRVRNPLPARGGTGPTPLARARMD
                     APQAFRRQERAVTPQNYADMAMRDTRVQRAVATRRWTGSWYTMFITVDRRGGAPVDAP
                     FEDSMTAFIDRYRLAGHDVEIDAPAYVALDIALHVCALPGYFTADVERRLLQVFGSAQ
                     GGFFDPDRFSFGDPVYLSAVVAAAMAVTGVSHVEPLRFQRLGRAPAGEITAGQITMAR
                     LEIARLDNDPNAPENGRLAFEVHAMGGQP"
     gene            complement(8685..9053)
                     /locus_tag="PK518_00980"
     CDS             complement(8685..9053)
                     /locus_tag="PK518_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HRM47512.1"
                     /translation="MSSYVAFPYRTDGRGRTHEAAREAWLHGLIEQLLFTLPGERVNR
                     PDFGCGLMQLVFAPNSPELAVTVQALVQASLQQWLGQLLRIDEVAASSEDATLTVAVR
                     YTVLETQQAGEAQFTRSLAS"
     gene            complement(9066..9386)
                     /locus_tag="PK518_00985"
     CDS             complement(9066..9386)
                     /locus_tag="PK518_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47513.1"
                     /translation="MPGPLLHLGATVLCAHGGQATPSAPVPRVLVSGQPVATQAAPWL
                     VAGCAFSPPQGNGPCVTAQWVVAATRVFAAGQPVLVMSGQAICTPTGTPLMPVAAQTR
                     VIGM"
     gene            complement(9397..9906)
                     /locus_tag="PK518_00990"
     CDS             complement(9397..9906)
                     /locus_tag="PK518_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HRM47514.1"
                     /translation="MNDKFYGKYRGMVLNNIDPMQIGRLQVQVPDVAGLLPATWAMPC
                     VPVAGVQNGMFALPMIGSGVWVEFEQGDPDYPIWVGCFWGSAAEVPALARATPPGVQA
                     ITLQTTLQNGLTVSDLPGPTGGIMLKSATGATLIVNDTGIYIQNGKGASLIMTGPTTN
                     INAGALTVV"
     gene            complement(9921..11063)
                     /locus_tag="PK518_00995"
     CDS             complement(9921..11063)
                     /locus_tag="PK518_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47515.1"
                     /translation="MFAQGIQLTLLVGPLVPLPVPRVVLDALQSVEVRTAAGSASGFQ
                     LTFTITARSPLNTIFLLAAGTNTGMATPPLRVLLILTMNGSAQPLFDGVMTHVEVQPG
                     GRGQAGTLTVTGEDVTKAMDMQDFSGLPFPAVPVEGRVALLCAKYAPFGLIPMVIPVL
                     FPDVQIPIDKIPAQKGTDLAYIQELARQVGYVFYVEPGPAPGTNIAYFGPEIKLGVPQ
                     PALNLDMDALTNVESLSFSFDPTKGVLPIVYIQNQLTRVPIPIPIPNLNPLQPPLGAL
                     PTPIANLKVLKDTAKMSPMRAISAGLAEAAKSQDAVSGSGSLNVLRYGRVLKARGLVG
                     VRGAGVAYDGLYYVSSVTSRLKRGEFKQSFELTRNGLVSITPNVPV"
     gene            complement(11066..11431)
                     /locus_tag="PK518_01000"
     CDS             complement(11066..11431)
                     /locus_tag="PK518_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRM47516.1"
                     /translation="MTDPIAAFMQANGLAQPAFAPDSRYHGLPTAQTQLADGRTVAYV
                     TRRFLPPPENFATIQTLTLAAGQRPDQLAAQYLGVAEQWWRLADANGAMQPEAMTAVP
                     GSSLRITLPEGVPPPPFEA"
     gene            complement(11434..12093)
                     /locus_tag="PK518_01005"
     CDS             complement(11434..12093)
                     /locus_tag="PK518_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47517.1"
                     /translation="MTTTPFSPKLIKGGIVLVDPTTAQVQRVIALQYNAESLKRELKG
                     QDVGGEAGGDRAEPTRFKGPAVETISFEADLDATDQLEFPDQHAATVAHGLAPQIALL
                     ESLSQPSSAQLSKVNSQASSGQLEIAPMLAPLLLLVWGASRVIPVKLTSFSVTEEAFD
                     PALNPIHAKASFGLRVLTVDDLGFASKGGALFMTYLQNREQLAAKAQPVSLSTLGVTG
                     V"
     gene            complement(12112..>13141)
                     /locus_tag="PK518_01010"
     CDS             complement(12112..>13141)
                     /locus_tag="PK518_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRM47518.1"
                     /translation="RAAVDGRTVMAPTAYAAVQSTLASAGTPLDKGTRGFMESRFGHD
                     FGQVRIHAGNGAAASARAVHARAYTLGRDIVFGANQYAPTTSEGRRLLAHELAHVVQQ
                     RAAPQQARLMRKPEASSPAQQHFYYESTEFGGRFDAAVDTRNHRVRLIMRLKLTDMGP
                     PQDKAERIARFSARAQAVIESTWSGIYALQSACHGDHEKFEAQVLVVFTSKAPHREIN
                     LWSDKGERSKSTEWQENDTAMKERQSPVLIDPKKPPSEKNLRMATFRQTTVAHEFGHL
                     MGLSHVVCDGSAERCYGITAEQKTDIMGYGSVVSKRDYAPFVRIMERYGQDNLPGTCN
                     SWRLVEPG"
     assembly_gap    13142..13241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13242..14246)
                     /locus_tag="PK518_01015"
     CDS             complement(<13242..14246)
                     /locus_tag="PK518_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47519.1"
                     /translation="MNAYARLQPRCACGGAAAGRCAACARRNIPGPQPKLAVSTPGDR
                     WEREADQAADQVLAAGGRPQLSRLASQPLQREGEDEKKKPPPSPVTEGLSTVASNLGE
                     NNPAFSKFTERLGDRFMAQPAPLSIGVPVFLGANYAFLWTMALADPAMRRQFDDFNLA
                     LLPGIVPQFPVKTFKYQILDGKQSRFAFEIGLDASKLMEAFNQGVLNTRVSSLKLDTG
                     GTLDTAGPRPVSLSALQVQVGLFGDGLQLSGGFRNGISPYPLPGPGGPGGDSSRVMAQ
                     SPALPDLYANQRDVRFMVLLDVPKLVQYFSPSAPSGPERLQRAAVDGRTVMAPTAYAA
                     V"
     gene            complement(14243..15466)
                     /locus_tag="PK518_01020"
     CDS             complement(14243..15466)
                     /locus_tag="PK518_01020"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRM47520.1"
                     /translation="MNTIPQHARVSASTPTGLWLQRKCACGGKVGSSGACADCQRRKT
                     LGWQTQLAVGAADDVYEREAERVAQQVAGGPALQQAGMPRIQRLSAAPGAASMTPDAS
                     PPSVETTLAAPGRALEPEVRQDMERRLGHDFSRVRVHTDAPAARSTQELDANAYTAGA
                     HIAFAPGRYAPRTCEGRRLLAHELAHVVQQGAGHPLGVQRDGKDKPTPAPRVVPPVAP
                     NQTQQKMIDDARRGAAVRTQLAMFRASGIEGAEALMRAKALARIKFDWPDPNMEQISD
                     VLRGMGGGVITAEAKVAGPGDPECGTRAGYVRGYRAPIVLCPAFFAKGTTPESRIRTM
                     VHEMAHLQRIGSADAGEAYYPVFDCDSPGAFESADSWANYVHCLSGQTPDANVVTVQV
                     PGGGSKAAPKKGGKP"
     gene            complement(15463..15735)
                     /locus_tag="PK518_01025"
     CDS             complement(15463..15735)
                     /locus_tag="PK518_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47521.1"
                     /translation="MNRIVVHIDRLVLRGVDHVDPAALQAALQAELRTQLGVGDVARQ
                     LTPHDGAAVLHVAPVRLRPGTAAAELGRAVAGGIAGGAPRRGGEAK"
     gene            complement(15732..16241)
                     /locus_tag="PK518_01030"
     CDS             complement(15732..16241)
                     /locus_tag="PK518_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47522.1"
                     /translation="MSPQSLRILLQPLLAGDLAAWQGLPAVSVADFDALYGPPTVRRE
                     EPLGALPAMRHDYAAGDDGTLAMWSRGGQAVMLAPAHLPSPAVLDTLPAPDAVLAHEI
                     LVPGHYAHEYLYCTRGLVLTVAQSLQGNERHIARCRGMAPLAAPREFGPDFYLAFEDR
                     VSWARLEDS"
     gene            complement(16238..18205)
                     /locus_tag="PK518_01035"
     CDS             complement(16238..18205)
                     /locus_tag="PK518_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRM47523.1"
                     /translation="MSVRTTATQRVADTANRDVMRLQRRKCGCDQAGEPAERFEGRQR
                     RAFGLQRKLAIGAADDPLEDEADRAAERALQNLPLPQGGAPAAQRLSRRAAPGACAGS
                     PAPESVQATLREPGMPLDAATRAEFGERFGHDFGSVRVHHGAMAALSAREVAALAYTV
                     GRHIVFDAGQYRPRSAAGRRLLAHELAHVVQQSAAPAVTLRRAEMRQGAVRVHIDYGP
                     IIFVTAANRPDRVVALMATLIGAPPSATQESTARALGIAAQDWLLFALQLLSQNRAAA
                     STLSGSTAVDRLLAYAPSARHRPLPDPDNEFVREVLRESGWSQTAQIERLPVPGAADR
                     TAVDKIVNPPPSSGSATDPLEVGEFKRRLPPALKHLVQQLDPARWTKRGTRSLSAFQA
                     LGDIVQQEARSFFAPYADAAIGNLYDLQPAWHASAHIFDVGALAPKQDQRIGYLLNRA
                     EIVGRSTAVTTIVNDANIFDQTHFDGSRADDRRELLAVVSALEGQKAIADAVDRLIQH
                     TGRKSGTGAATTIGLVTEFNADTASACTDHWRGIATLCHEVLHALVHPGFNAAAATVR
                     FPQIIREGFTEVLGAQLFNQRVRPKAASTAAFKQQLEAGVSGAPCPAPAADAIGYGAA
                     GAGAEAIRARVGDNRFRAAYFLGRPELAGIT"
     gene            complement(18202..20136)
                     /locus_tag="PK518_01040"
     CDS             complement(18202..20136)
                     /locus_tag="PK518_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM47524.1"
                     /translation="MNAPERVTDWTAANQQLLVAEFARLRALLGEPGEGGNAALASAR
                     TALPAPAAIDTLAALFQLSPFERELVLLAAGVEMDARLAALCAQAGTAQRPWASFGLA
                     LAALPDPHWSALAPDAPLRHWRLLEIDEGAGLTSARLRIAERVLHYLAGLDHLDYRLQ
                     SLLRPVSDDAPPLAAAQQAGLARLLARWRERPRFILLEGDDAGGQRDLAAALAAELDA
                     RLYCLQGFDLPMLAAEQAQFAALWSRDAVLLGAGLLIQCEDAAGAEQPAAALRLAERL
                     PGLVLLSGRTWPATEFPCHRLRLDPPEAPERRRLWQQALGPHRAARLPPAALDGLASQ
                     FRLGARRIAEIAAEAHAAGQADDTATLCRAARERAGRSAVLPGLLQQVDACAGWNQLV
                     LPPAQLQTLHQIAAHARHRLTVHHRWGFADQGARGLGLATLFWGDSGTGKTLAAEVMA
                     TELDLALLRVDLSAVISKYIGETEKNLRHVFDAAEQAGAVLLFDEADALFGKRSEVKD
                     SHDRYANIEVSYLLQRMEAYGGLAILTSNHKAALDPAFQRRLRFVVHFPFPDQAQREG
                     IWRAVFPKAAPLASDMDFERLARLDVAGGSIRNIALAAAFLAAEAGSDIGMAQLQQAA
                     RMEGAKRERPFSEAELRGWT"
     gene            complement(20133..21470)
                     /locus_tag="PK518_01045"
     CDS             complement(20133..21470)
                     /locus_tag="PK518_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HRM47525.1"
                     /translation="MSTALAIAGVTAVLRDLLNDGLINHNISGLLGSSVTVSVQAPDR
                     VVTGSTPEASQLNLFMYMVTPNLGWRNEGLPGRDGSGRTRLTNPPLALDLHYLLSAYS
                     GADLHAEILLGYAMQLLHETPVLTREAIRTSLIPSPSVGNALPPALRALADSGLAEQV
                     ELIKINPATQSSEEMSKFWTATQSHLRPSAAYTASVVLIQAQRPARSALPVLTRGPRD
                     AQGREAGIAVQPSLAASMPLLTRAQPPAGQPVAGIGDLVVLHGQSLDGADRRVTLNNE
                     PLQVALELAVQPPLPPDRPAATTAAVSLAGQAAALPVGIYQVTLQATQPDLLNQPRRM
                     SSNRLALALAPRITNLPATVARAGDGSATVTLNFTPELRTGQHATLVLGGSEVLPLTA
                     GATPTSLQFRVANAVPGTYLARLRVDGIESPIVDMDLQPGDTPVFLPITVSIT"
     gene            complement(21467..21790)
                     /locus_tag="PK518_01050"
     CDS             complement(21467..21790)
                     /locus_tag="PK518_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47526.1"
                     /translation="MAVHADPPPLLPLNADRNTANTPAASSPPLGKAQPQVAAAPHAL
                     RGLAQLSAHAPPRSEPPEVHIHIGRIEVTAAVTPEPQAPRRPARERAQPLSLDAYLAQ
                     RKGRS"
     gene            complement(22219..22941)
                     /locus_tag="PK518_01055"
     CDS             complement(22219..22941)
                     /locus_tag="PK518_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47527.1"
                     /translation="MRVLGAAELLSLWERGMSRHALDRAALLAATAHPNWPADAITDL
                     PLGAIGSSLLRLRAANFGPRIEAHADCQQCGERLAVALEVAELLHGAAPEDGTAPESP
                     TVTVADGLQVRASSLRDLAAVASLAPDAAADALLARCTLNGRALPLDAATRAQVEDAL
                     EALDPQADLAFTLNCVACGHEDQAQLDTAALLWDEITARAGALLHEVHRLASAYGWSE
                     AQILALSPARRAQYLALVEGMS"
     gene            complement(22958..23482)
                     /locus_tag="PK518_01060"
     CDS             complement(22958..23482)
                     /locus_tag="PK518_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRM47528.1"
                     /translation="MAQFSVNATRFDPYKNFKFRVKWDGKYVAGISKVGALKRSTEPV
                     EHREGGDPSTSRKSPGRTKYEAITLERGVTHDTEFEKWANKVWNFGSGLGAEVSLKDF
                     RKDLIIEVYNEAGQLALAYKVFRCWVSEYQALPDLDANANAVAIQHLKLENEGWERDY
                     DVSEPSEPSFTEPV"
     gene            complement(23498..25393)
                     /locus_tag="PK518_01065"
     CDS             complement(23498..25393)
                     /locus_tag="PK518_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HRM47529.1"
                     /translation="MPSALSYPGVYLEELPSGVRTLTGVATSITAFVGYTRMGTPDKA
                     VPITSFSDFERAYGGLDRDSPVSYGVRQFFANGGAQAIIVRVATGYASAAWTFNGGSP
                     AAHVLDVMTASPGAWGNDLRITVDRAAALNPDAQFNLIVSQLQADGVTLVPLETHRNL
                     DMNPSSARYVESVVNNASALVSAKCRGGLTFNQTGFAVSKTVTFPLTATDLVIGGVVD
                     GSQAFRLTLAGPAPASMAALVAAVNTAIGTAGLTALLQASESLADGSAGAGCLKLASL
                     TAGESSGVVIAGGAFGGLSGSIHLGLANGGREFTGNAQHRPAEVANLVPPTKGVDGTR
                     AGANEIVGSASGKTGMHALQDVDLFNLLSIPETYDMTDLQAAAVIPAAVDLCEKRRAF
                     YIVDAPAGKNLSNIASWANGVTQSRNAAVYFPAVKLVDPLDELRPRAMAPSGTLAGIY
                     ARTDANRGVWKAPAGTDATLNGVLDLAAPLNDLENGQINPKGVNALRNFPAYGRVAWG
                     ARTLKGADAQADEYKYVPVRRLALFLEESLYRGTQWVVFEPNDEPLWAQVRLNIGAFM
                     QGLFRQGAFQGKAPREAYFVKCDSETTTQADINLGVLNVIVGFAPLKPAEFVVIKIQQ
                     MAGQIQA"
     gene            complement(25587..28232)
                     /gene="mutS"
                     /locus_tag="PK518_01070"
     CDS             complement(25587..28232)
                     /gene="mutS"
                     /locus_tag="PK518_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRM47530.1"
                     /translation="MTGKTAPAPRPAEADFSGHTPMMAQYLALKSQHPDTLLLYRMGD
                     FYELFYADAEKAARLLDITLTQRGQSAGQPVTMAGVPFHALENYLARLIKMGESVAIA
                     EQVGEVGASKGPVERKVVRVVTPGTLTDSELLPDKSESMLLAVHQGARARCGLAWLSV
                     TQGVVQLAECTQAELGTWLARIAPSELIYSAGITQRFEEQLNQLRQGGAFACPMNLRP
                     DWQFDAALGQRKLLEHLGAASLQAWNAEGLADAHAAAAALLQYAEHTQGRTLTHVHGV
                     RVQQGDALIHLPAATRRNLELVKTLRGEDGPTLFSLLDSCMTGMGSRLLKNWLLEPER
                     NRTAARQRLAATQVLRGAGSGQGPWQLLRGELKGVSDVERITARIALRQVRPRELVAL
                     CKTLQKAELLALTSQAPEPYLAQIFAELQPPADCTALLHAAILEEPAALVRDGGVIAP
                     GFDAELDELRAIQNNCDDFLLDLETREKARTAIPNLRVQFNKVHGFYIEVTASHVDKV
                     PEDYRRRQTLKNAERFITPELKAFEDKALSAQERGLAREKWLYEQVLDQLQPHVARLT
                     RLAQALAQLDVLCCLAERSLTLNWCAPQFVNEPCIDIEAGRHPVVEGRLAETSSGAFI
                     PNHTRLNANQRMQIITGPNMGGKSTYMRQVALIVLLASMGSHVPASACRLGPIDAIHT
                     RIGAADDLANAQSTFMLEMTEAAQILHGATPHSLVLMDEIGRGTSTFDGLALASGIAT
                     HLHDKTRAFTLFATHYFELTELAARHAHAVNVHVGATEAGHDIVFLHEIQAGPASRSY
                     GIQVAKLAGVPAAVLNHARHTLAALEERAGEDDLQVDLFAPPPAPEIQAVSPVESALA
                     QINPDQLSPREALDALYQLKRLTTN"
     gene            complement(28294..29874)
                     /locus_tag="PK518_01075"
     CDS             complement(28294..29874)
                     /locus_tag="PK518_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRM47531.1"
                     /translation="MPRHSQATSRRRLLQQAVWAATATQLPRWAWARQPLRHDPFGLG
                     VASGEPQPDGVVLWTRLLPEHPADAGAPWAGPLSVRWEVADDAQFQRIVQQGQATALP
                     ELAHSVHVEVTGLQPGRWYHYRFMLDDAVSRTGRTRTAPAADALAPRLRLVYASCQRW
                     ENGYYAAWRHACADEPDLVLFLGDYIYENPSPKDTSGLARVHDLPLPQNLADYRARYA
                     LYKSDPDLQAAHACAPWMVTWDDHEVQNDYAGNHGLGDARWPPLRAAAWQAYYEHMPL
                     RASALVSSSFQGLQLYRRLAWGRLARLHMLDARQYRDRQACRPPGSRSAGSVHPGACS
                     ELAAPQRSFLGWQQEAWLQQGLAQDGREHAARWSVVAQQTLLSPRHYPSGQLPADSWD
                     GYPAARARLLNALAQHPPRNTVVLGGDIHQNYVCNLMAPPEAGKAGLILASEFCGTSI
                     SSRAGTTQDRLDALRRSNPHVLLARSEERGYGLCDITPEHWTTQLRALRNPLDAGSDA
                     YTLARFVVEDGRAGPQSA"
     gene            complement(29894..31414)
                     /locus_tag="PK518_01080"
     CDS             complement(29894..31414)
                     /locus_tag="PK518_01080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM47532.1"
                     /translation="MDSAKSPSDIARETLKLLATRRLTPSPDNYRALYEEVAGTRSAP
                     QFPATQLRQILRVMPCQTPGQKRQAELLEKAIAQQDWSMLQNVLVGYAKLGLAPAPVP
                     PVHSASIDHLPHGLAQNLVRMVENALPALGEDDPRIHELAAQLTAFLREPTPPVATAE
                     QMLRNYSYRLSFAAEDQAAIRQSLLGLLRLVFENISALSIDDAWLHGQAEALLTASTP
                     PLTLRQLDDVQQRLKDVIFKQSEAKDRSMQAQSQMRELLATFIERLAQMDESSGSYSE
                     QMERCAERISQATELKEITPLLEEVMAATRAVALNSRLTRAELQDLRERAETTHQEMD
                     LLRQELDRASIQARHDPLTGSLNRKGFDEAIGREIARARRQGKPLCLALLDLDNFKSL
                     NDRLGHEAGDDALKHLVLVTREVMRPQDQLARYGGEEFVLILPDTELTEGVKAMERLQ
                     RELTKRYFLKEKERVLITFSAGVTQLEGDDHADAIRRADQGMYLAKRAGKNRVLAA"
     gene            complement(31599..31807)
                     /locus_tag="PK518_01085"
                     /pseudo
     CDS             complement(31599..31807)
                     /locus_tag="PK518_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465061.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            31944..32726
                     /gene="surE"
                     /locus_tag="PK518_01090"
     CDS             31944..32726
                     /gene="surE"
                     /locus_tag="PK518_01090"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRM47533.1"
                     /translation="MKILISNDDGYQAPGIVALHDALKAIADVEVVAPEHNNSAKSNA
                     LTLHSPLYVHRAANGFRYVNGTPADCVHIALTGLLGYRPDLVVSGINNGANMGDDTIY
                     SGTVGAAMEGYLFGIPSIALSQVDKGWGEIDSAARKACEIVEQMQRHHLVGQSPWLLN
                     INIPNMPFEALRSLRLCRLGRRHAAERVIEQQSPRGELMYWIGGAGAAKDAAEGTDFH
                     ATAHGHVSMTPLKVDLTDHDGLGYWAQTAARLAVPGLSGGGL"
     gene            32726..33499
                     /locus_tag="PK518_01095"
     CDS             32726..33499
                     /locus_tag="PK518_01095"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRM47534.1"
                     /translation="MQRRPGFPARLDRPQAGAMTPTVPAARLARETQPPKPQGVGLDS
                     AAVRARMVQRLAAAGIAAAPVLQAMGAVERHRFVDTALANQAYEDTSLPIGLGQTISK
                     PSVVARMIELLLGAECARAGALGRVLEIGTGCGYQAAVLARLAREVYTIERLRALHEK
                     AREHLRPLRLANVHLILGDGMLGYAAGAPYAGIISAAGGDSLPMQWCEQLAVGGRLVA
                     PMAGADGRQLLLVVDKTLQGLKQSVLEAVHFVPLKSGVA"
     gene            33511..34431
                     /locus_tag="PK518_01100"
     CDS             33511..34431
                     /locus_tag="PK518_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRM47535.1"
                     /translation="MLVSRGLVAWGMVALAGMGLVGCGTPVNKAPVVDRGTFANGGAT
                     PPPAAPEVKPLPGAENAGKPGYYTVKPGDTLIRIGLDNGQSWKDIARWNNLDNANLIE
                     VGQVLRVVPPGAPSSAVASDTGVVTRPVTTSPVAPSSSASAPKAGPPTAAASTPAAPV
                     EPIAAPAPAPAPAPAPAPAVAGGVDDMHFIWPSSGSLLAGFDEARNKGFDIAGKAGDP
                     VLAAADGRVVYAGAGLRGYGNLVILKHNNTFLTAYAHNQTLLVKEDQNVRRGQKIAEM
                     GSTDADRVKLHFEIRRQGKPVDPARYLPAR"
     gene            34428..35462
                     /locus_tag="PK518_01105"
     CDS             34428..35462
                     /locus_tag="PK518_01105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM47536.1"
                     /translation="MSTPVAGDGFQWPDEESEPQDSGEACASGQADADLELRQVEVSA
                     VQHQVRLDKALAELVPEFSRSYLQQLLSQGCVRLNDQVAHKPAHKVRAGDRVVVEMRP
                     TQQSQAFKPEQLPLDVVYEDAHLLVVNKPAGLVVHPAPGNWSGTLLNALLGRDAQALQ
                     VPRAGIVHRLDKDTSGLMVVARSRPVMDALVRMIAAREVSRQYLALAWGRWAGAAQRV
                     VQAPIGRDPRNRLRMATVDLQQHPGKPARTDFELLVGNDEVCLVRCTLHTGRTHQIRV
                     HMASLRHPLVADGLYGGALGGVLQRQALHAFRLAFQHPVTGQAMELRAPLPADMLQAL
                     DFWALGYNGG"
     gene            35626..36327
                     /gene="scpB"
                     /locus_tag="PK518_01110"
     CDS             35626..36327
                     /gene="scpB"
                     /locus_tag="PK518_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRM47537.1"
                     /translation="MNSVDAKRVLETALICAPQPVTVRELRTLFDDALGSDTVKMLLL
                     QLQQDWALRGVELVQVATGWRFQSRPEMREYLDRLHPEKPPRYTRATLETLAIIAYRQ
                     PVTRGDIEDIRGVTVNSLIIKQLEDRGWVEVIGHRETVGRPALFATTRQFLDDLGLQS
                     LDQLPTLDDPGVQATVFEALADAPEVQRPDKLEMKVALDIAETPAQGDADMADAPVES
                     GGVENEEADDGVTGT"
     gene            36329..37654
                     /locus_tag="PK518_01115"
     CDS             36329..37654
                     /locus_tag="PK518_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM47538.1"
                     /translation="MNDTHKDLDAAQPAAQAPAGSVDEPRARRPRQRKPGQIADSASF
                     AQQPMAQQAEEPGQKPQRDSRGPQGRRGGKSSADREGYRFADVVSGQLDQDETDTEVA
                     PAKRVLLPQPETPKLHKVLAQAGMGSRLEMEQLILEGRISVNNEPAHVGQRVQFGDQI
                     KVNGKPIRFRIAPPPTRVIAYHKPAGEIVTHDDPQNRPTVFRKLPRLVQGKWQSVGRL
                     DLNTEGLLLFTSSGELANRLMHPRFGLEREYAVRVLGALSDEEKQRLLDGVQLEDGMA
                     AFGSIDEGGGEGANCWYRVTISEGRNREVRRMLEAVGHAVSRLIRIRYGAMLLPRGLK
                     RGAWMELDEHDIQALMQAAGAPLDGAAAGRQPGGGGQGNNAHRGERKPMGGKMAGGGR
                     SKSLARDGRSAGNTQPDPMKTSVGYIGADSFLRQRQQQRSGGGARRGRR"
     gene            37732..38157
                     /gene="ndk"
                     /locus_tag="PK518_01120"
     CDS             37732..38157
                     /gene="ndk"
                     /locus_tag="PK518_01120"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRM47539.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYARFEAAGLKIAAARMIHLSR
                     AEAEQFYSVHKERPFFKDLVDFMISGPVMVQVLEGENAVLKNRELMGATDPKKADAGT
                     IRADFADSIDANAVHGSDAAETAAVEVAFFFPGLNVYAR"
     gene            38246..39370
                     /gene="rlmN"
                     /locus_tag="PK518_01125"
     CDS             38246..39370
                     /gene="rlmN"
                     /locus_tag="PK518_01125"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRM47540.1"
                     /translation="MTTNLLDFDLDAMAAFCERLGEKRFRATQLFRWIHQRGARDFDQ
                     MSDLAKSLREKLKGCAHVTGLQAISEHVSSDGTVKWLFDVGGGNAVETVFIPEDDRGT
                     LCISSQAGCAVGCRFCSTGHQGFSRNLASGEIIAQLWHAEHVLRQRRGDGERVISNVV
                     MMGMGEPLQNYSALVPALRIMLDDHGYGLSRRRVTVSTSGVVPMMDRLGADCPVALAV
                     SLHAPNDALRDNLVPLNRKYPLHELLAACRRYLDHAPRDFITFEYCMLDGVNDQPEHA
                     RELIELVGRRAADGGVPCKFNLIPFNPFPASGLHRSPAAAVSAFAKLLSDAGIVTTVR
                     KTRGDDIDAACGQLAGDVKDRTRVHERMAKLRVIEIKPVH"
     gene            39399..40223
                     /gene="pilW"
                     /locus_tag="PK518_01130"
     CDS             39399..40223
                     /gene="pilW"
                     /locus_tag="PK518_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HRM47541.1"
                     /translation="MRLWGGGGIRHGLLAACLAVGAAALGGCAHQAGLDSGVGAAEPV
                     SASGDTDVRRRARIRLELAANYLQMGQTGVALEEVQQALATDPSYADAYHLRGLVYMA
                     LADLQGAEDSLKRAQAMRPNDPDIMHNYGWLQCQRQQYAQANQLFERALASPTYASRS
                     KTLMSQGLCYQRAGQVAEAEKTLLRAYEIDAGNPLVGYQLASILLAREEPKRAQFYIR
                     RVNNGEFSNAASLWLGVKVERALGDSVAMRQLGEQLHKRFPDAKETLAFERGAFHE"
     gene            40216..>40951
                     /locus_tag="PK518_01135"
     CDS             40216..>40951
                     /locus_tag="PK518_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM47542.1"
                     /translation="MSESIGEHNADQSVPAPAPNAGGMSAGTWLRQVRESAGLHIDVL
                     AGALKVPVAKLQALEADDYAAFPDAVFMRALASSMCRALKVDAAPVLALLPQGAPIHL
                     PPDRAINASFKDSGRRLGKGGSIERPKSRAMGLAVVALLLGALAVAFLPLGGDGKQDQ
                     PALAMTPAPQAPAAPVVAAQEPEAVVPAAEPTGAAPEAQPADGTPVAQEPAAAVATAA
                     VQGALVIRAHKESWVQVRDAGGRVLLQ"
     assembly_gap    40952..41051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41052..41264
                     /locus_tag="PK518_01140"
     CDS             <41052..41264
                     /locus_tag="PK518_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRM47543.1"
                     /translation="HKESWVQVRDAGGRVLLQKALAAGESYSAEGSPPWRVVIGRADA
                     TEVIVRDQPMDLKAVSRENVARFEVK"
     gene            41265..42527
                     /gene="ispG"
                     /locus_tag="PK518_01145"
                     /gene_synonym="gcpE"
     CDS             41265..42527
                     /gene="ispG"
                     /locus_tag="PK518_01145"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRM47544.1"
                     /translation="MNPTDAPIAMAAPLARRSRQARIVWGSNVVTVGGDAPVRVQSMT
                     NTDTVDAIATAIQVKELAQAGSEFVRITVNTPEAAAAVPYIREQLDRMGCDVPLVGDF
                     HYNGHRLLTEFPDCAQALSKYRINPGNVGKGDKRDRQFGQMIEAAVRWDKAVRIGVNW
                     GSLDQELLAGLMDVNGRRSQPWDARQVMYEALITSAIESARRAEEMGLRGEQIILSCK
                     VSGVQDLIAVYRELARRCDYALHLGLTEAGMGTKGVVASATALSILLQEGIGDTIRVS
                     LTPQPGEARTQEVVVAAEILQSLGLRAFVPSVTACPGCGRTTSTTFQELAKQIDDYLR
                     AQMPVWRERYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKA
                     MTLRGENIVREFQDIVDGYIDKRFGLGA"
     gene            42606..43895
                     /gene="hisS"
                     /locus_tag="PK518_01150"
     CDS             42606..43895
                     /gene="hisS"
                     /locus_tag="PK518_01150"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRM47545.1"
                     /translation="MQKLVAIKGMNDILPPDSARWEWLEEKVRSLMARYAYRNIRTPI
                     VEPTPLFVRGLGEVTDIVEKEMYSFEDRLNGEQLTLRPEATAGVVRAVVEHNMLYDGG
                     KRLYYMGPMFRHERPQRGRYRQFHQIGAEALGFGGPEVDAEIILLAHALWAELGLTNV
                     RLELNSLGQPDERRAHRAALIAYLEQHQDVMDEEARRRMYSNPLRVLDTKNPAMQDMV
                     NAAPRLIDFLGEASLRHFDTVKAILDANGVAWSINPRLVRGMDYYNLTVFEFITEQLG
                     SQGTICGGGRYDYLIEQIGGKSAPAVGWALGVERVLELLKEQAAQAPQPAADAYAVVP
                     DAAALPQVMVCLQQLRALGVAVQMHSPANSAEGMGSMKSQFKKADASGARFALIFGSD
                     ELARGVVTVKPLRDGGEQVERPLATPADWAASLQSPR"
     gene            43910..44590
                     /locus_tag="PK518_01155"
     CDS             43910..44590
                     /locus_tag="PK518_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM47546.1"
                     /translation="MANNFDLQEQEQLEELKHFWNKWGTPITWALVVIMGSFAAWNGY
                     RLWQGRQAQQATALVEAVELAAQTGDMARVEQAFGDLQSGYGGTTQAGQAGLLAAKAL
                     ADAGKWDAAKGVLTWVAEKSSDEGYVALARLRLAGVLMEQKAYDAAMAQLSGSMPAEF
                     AGVVADRKGDVLALLGKNQEAIAEYRRAYQAFGDSVEYRRLVEAKLNALGAQAQNVAV
                     AAPAGSAQ"
     gene            44623..45735
                     /gene="bamB"
                     /locus_tag="PK518_01160"
     CDS             44623..45735
                     /gene="bamB"
                     /locus_tag="PK518_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HRM47547.1"
                     /translation="MTLSLAALLGASGCSLWGGSPKPKPAELGPNVPVLGVRQAWTAK
                     IGAMDGLALDVHVNANTLTLAAANGEVAAIDARTGGDVWRTQLNLPLAAGVGSDGRWA
                     AVVSQGAQLIVLDGGREIWRKPLTAQAYTAPLVAGNRVFVLAADRSVSAYDAATGYRL
                     WGQQRPGEPLILRQAGVLMAVGDTLVAGLSGRLVGFNPDNGVVRWEAPLASPRGTNDV
                     ERLVEIVGHTSRVGDNLCARAFQATVGCIDAARGAVLWTQPAGGSEGLDGDAEMLFGT
                     ESNGTVVAWQRADGKRAWTSERLQFRKLTAPLLLGRSVVIGDDAGLVHLLSRADAAPL
                     NRLATDGSGIAAAPVAAGDTLVVVTRKGGVYGFRPD"
     gene            45754..47097
                     /gene="der"
                     /locus_tag="PK518_01165"
     CDS             45754..47097
                     /gene="der"
                     /locus_tag="PK518_01165"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRM47548.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRMTKSRDAIVADFAGLTRDRHYGN
                     GRQGQHEYIVIDTGGFEPDAGSGIFREMAKQTRQAVAEADVVVFVVDVRGGLSAQDHD
                     IANYLRRLGKPCVLAGNKAEGMQDSPHLAEFYELGLGEVHPVSAAHGQGVRSLVELAL
                     EPLALPEVDECEAGAEANVIRLAVAGRPNVGKSTLINTWLGEERLVAFDLPGTTRDAI
                     SVPFERNGQRFELIDTAGLRRKGKVFEAIEKFSVVKTLQAIESANVVLLLLDATQGVT
                     DQDAHIAGYILESGRAVVIAVNKWDAVDDYQRQQLERSIESRLSFLKFAPLHFISAIK
                     RQGIAPLWTSIAQAYKSANRKMPTPVLTRLLHEAVQFQTPKRAGGFRPKLRYAHQGGM
                     NPPVIVIHGNSLEHVSDAYKRFLEGRFRKEFDLVGTPLRIEMKTSSNPYTDKNGA"
     gene            47175..47423
                     /gene="hfq"
                     /locus_tag="PK518_01170"
     CDS             47175..47423
                     /gene="hfq"
                     /locus_tag="PK518_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRM47549.1"
                     /translation="MSNKGQLLQDPFLNALRREHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTIVPGRAVNFSTVETAEGSNQ"
     gene            47487..48668
                     /gene="hflX"
                     /locus_tag="PK518_01175"
     CDS             47487..48668
                     /gene="hflX"
                     /locus_tag="PK518_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRM47550.1"
                     /translation="MSSKPSSDARTTPVLLIGVDFGAPHFDADLQELGLLAQTAGLNP
                     VARLTCKRKAPDAALFVGSGKADEIRTLAQMHGAQEVLFDQALSPAQQRNLERHLQLP
                     VNDRTLLILEIFAQRARSHEGKLQVELARLQYLSTRLVRRWSHLERQQGGIGMRGGPG
                     ETQIELDKRMIGDAIKRTRERLLKVKRQRATQRRQRERRDTYNISLVGYTNAGKSTLF
                     NALVKARAYAADQLFATLDTTTRQLYLGEAAGSASLSDTVGFIRDLPHGLVDAFQATL
                     QEAVDADLLLHVVDAANPDYPEQIAQVQMVLAEIGASDIPQLLVFNKLDAMALESRPK
                     QLQDMYALDGRKVPRIFVSARQGEGVAALREQLAQVVASAASPNSMSPDQATELPNPH
                     V"
     gene            48721..50094
                     /gene="hflK"
                     /locus_tag="PK518_01180"
     CDS             48721..50094
                     /gene="hflK"
                     /locus_tag="PK518_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRM47551.1"
                     /translation="MNLPNRASRWALLPERIRGMFNLNDPRWGRGDDKGESDERPQQP
                     ERPNNDRPQGGRGRDQGPGGQPPDLDELWRDLNRKLSGLFGGGNGGGRGRMPGGGNGG
                     GGLQPDMKNAGVGVGLIAAIAVLIWLGTGFFIVQEGQQAVITQFGKYKSTVNAGFNWR
                     LPYPIQRHELVFVTQIRSADVGRDTIIKSTGLRESAMLTEDENIVEIKFAVQYRLNDA
                     RAWLFESRNPADAVVQVAETAVREVVGKMKMDTALAEERDQIAPRVRNLMQSILDRYK
                     IGVEVVGINLQQGGVRPPEQVQAAFDDVLKAGQERERAKNEAQAYANDVIPRAVGTAS
                     RLGEEAAAYKARIVAQSQGDAQRFAAVLTEYQKAPQVTRDRMYIESMQQIYSNVTKVL
                     VESRQGSNLLYLPLDKIMQGVSQTLPAATPEAPPAGSTGTAAPSQFPSDSRITRDPNR
                     SRERESR"
     gene            50104..51027
                     /gene="hflC"
                     /locus_tag="PK518_01185"
     CDS             50104..51027
                     /gene="hflC"
                     /locus_tag="PK518_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRM47552.1"
                     /translation="MNRIGFFVSSILVLLGLASSMMFVVDQRQFGVVYALGQIKEVIT
                     EPGLNFKLPPPFQNVRYIDKRLLTLDSSDTESMLTAEKQRVVIDWYVRWRISDPQEYI
                     RNVGLDESAGAMQLNRVVRNAFQEEVNRRTVRELLSSKRDALMEDVKREVLEAVRGAK
                     PWGVDVVDVRITRVDYVEAITESVYRRMEAERKRVANELRSTGAAEGEKIRADADRQR
                     EIAIANAYRDAQKIKGEGDAEAARQYAEAFGRDPQFAQFYRSLEAYKASFNRKSDVVV
                     LDPSSTDFFKAFRGGAAPGTLAAPGAVPRKP"
     gene            51048..51239
                     /locus_tag="PK518_01190"
     CDS             51048..51239
                     /locus_tag="PK518_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HRM47553.1"
                     /translation="MDWADSFWMALGLVLVIEGIFPFVSPTGWRQMFTQILQLRDGQI
                     RFCALLSMLGGALVLLLLS"
     gene            51308..52456
                     /locus_tag="PK518_01195"
     CDS             51308..52456
                     /locus_tag="PK518_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRM47554.1"
                     /translation="MSAWVLPDHIADVLPSEARHIEELRRGLLDTARSYGYELVMPPL
                     LEHLDSLLTGTGEALDLQTFKLVDQLSGRSLGLRADTTQQVARIDAHLLNRKGVTRLC
                     YCGPVLHTRPDRPHATREPLQFGAEIYGHPGMEADIEAVQLSLECLRGAHVQDVSVDL
                     ADVRIVRSLLAGVPVGWQTLGRVHGALAAKDISELTSLTRDFPAASRQGLLALLQLYG
                     DIGVLDEAEIALKSIAGVRNVLSDLRALASSIQGARVTFDLADLRGYAYYSGARFAIY
                     TQGASDALVRGGRYDEVGAVFGRNRPAAGFSLDIKQLVSVVSPRPLKAAIRAPWGEGQ
                     GANAAIASLRSQGETVVCMLPGHGSEVDEFRCDRELIRVGDRWVVQAI"
     gene            52492..53868
                     /locus_tag="PK518_01200"
     CDS             52492..53868
                     /locus_tag="PK518_01200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRM47555.1"
                     /translation="MKTSKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNA
                     GHTLVINGVKTALHLIPSGIMRPGVKCYIGNGVVLSAAKLFEEIEGLEKAGVEVRSRL
                     RVSEACPLILPFHVALDVAREAAREHGGVEKIGTTGRGIGPSYEDKIARRALRVQDLK
                     YPERFVAKLRELLALHNHVLSTFLGSGSFQFSDALRPYMKDGQVQFDPVFAEAMRHAE
                     MLRPMMADVSRELNEAHAAGANLLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSG
                     VGPGMLHYVLGITKAYCTRVGGGPFPTELEWEKPGTPGYHMSTVGAEKGVTTGRSRRC
                     GWFDAALLKRSAQVNGLSGLCITKLDVLDGLEDLMLCVGYDLDGERIDLLPMGADDIT
                     RCKPIYEKMPGWADSTVGVTSYDALPVQARNYLERIEQVTGVPIAMISTSPDRDHTIL
                     VQNPYAAG"
     gene            53940..54464
                     /locus_tag="PK518_01205"
     CDS             53940..54464
                     /locus_tag="PK518_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HRM47556.1"
                     /translation="MLTEDGKHLYVSYDEYHNLIEKLALKVHQSGWEFDTILCLARGG
                     LRPGDILSRIFQKPLAIMSTSSYRAEAGTVQGHLDLARFITTPKGEIAGRVLLVDDLA
                     DSGHTLRAVIDMLKTNYAPITELRSAVIWTKGMSTFTPDYSVEFLPTNPWIHQPFEGY
                     DSLVPEKLAEKWRW"
BASE COUNT         8437 a        17508 c        19268 g         9122 t
ORIGIN      
        1 ccgacaggcc tgccagttgc gggctgcctg ccacgggctg cgccgtccag ctggcgccgc
       61 cgttgccggt ggccagcagc gtgccgccgt cgccggcggc ccagccggtg ttggcgtcac
      121 gcatgcgcag ggtgcgcagg cgggccgtgc tgccgctggt cgccgtgctc caggtggcgc
      181 cgccatcgcc gctcacgagc agggtgccac cgtcacccgc ggccagggcc tgggcaccgt
      241 tcaagaccac ggcacgcagc gtggcctggc tgccgctgcc gaccttggcc caggtctggc
      301 cgccgtcggc gctgcgcacg atggcgccgc cctggcccac ggccaggccg ttctgtgcgc
      361 tggtgaaggc cacggcatgg agccgcgcca cgccggtggc gcgcgccgtc caggtgacgc
      421 cgccgtcgac cgtggccagg gccaggccgc catcgccaac ggcccaggca tgctgggcgt
      481 cggtgcaggc cacgccgcgc agcgcctgca gcgtgccgct ggcctgggcg gtccagctgc
      541 tgccggaatt gctggtgcgc aagatggtgc cgccatcacc cacggcccag ccggtgctgc
      601 cgctgcgtgc cagggcgcgc aggtggcgcg tgctgccggt gttggccaca gtccaggcgc
      661 cgccgccgtt gctgctgacg agcagcacac catcctggcc ggcaatccag atgctgttgg
      721 catcctgggc gtgtacggcc agggcggcca tgccgcccgc cagcgcaaag cccgcgccct
      781 cggccgggcc gccgctggcg ccgatcacat cgcgcgccag atgttccagg cgctggcgcg
      841 tgatgtcgcc ctgctcgttg aagtcggcat cggtgagcac gcggccctgc tgcagccgca
      901 ctgccgtgta gtgcttgcgc gcgtcgaagg tttcgcggga gagatcgcct tgcatggtgg
      961 tacctccttg ccttgttgca tcagttggcg taatgcagcc cggccgtcag gcccaggcgc
     1021 aggtattcgt cgagcgcgtc gcgcaggttg gcctcgcgct ggggctggcg cagctgctcg
     1081 taggcgccca tctcggcgcc actctcggcg ccggtgcgta tctgcaccgc gcagcgcaga
     1141 tccagctgac ccagagccgg gtcgccatag cggcgtgcgc tgaacagcgg gcgtaccgtg
     1201 gcctcgacct cggcgcgcag ggccgcctcg tccgcgcccg tcggcggggg caggccggcg
     1261 gccgctgcct tggcgcgcag cgccgcgatg cgggtgtcgc tctcgagctg cggctggcag
     1321 cggtagcgcc gcggcacctg ggatgcgggc ggcacatagc aaaagcgcac gcagcccgtc
     1381 tggcgccgca cggccgtgac aggcgcgtcg aagatgcagt cgctggccgt gagcgtgagc
     1441 ccgctcaccg agccaaggac ggtcgtgcgc tgcagatcca ggggggcgtc gggggcattc
     1501 agtgccggct gtggggccgc gccctcgtcc agcaggccgg cgatgctgtc ggccagggtg
     1561 atgctggtga cagggccggc cgccgcgatg ggtgccgtca ggctgtgcag cacgcgcagc
     1621 gccaggcgcg ggctgtcgcc agccaccgtg atgtgggtgg tgctgggcag catggtgcaa
     1681 tgcgccagct ccacctggcc cagcgggccg gagaggctga gtggcccctg cagcagcagc
     1741 ccgttgatga acagcgcgcc ggggttgggg ctggccggcg cggcctggcc caccacgcgc
     1801 aggctgccct gccagtgggc gcgtgtatcc cgcgcctcga agcggccggg caggcgctcc
     1861 agggtgccgg gcagggcgcc gggtacgggc accagcggcc attggccgga gacgatcatc
     1921 agacgcgagc cctcggccag gttgatggtc agcggcgccg cgtcagtctc gctcaggctg
     1981 tccatgacaa cgatcacgcc ggtgcggccc gcaggcaggg cgttccaggc cgtgacggcg
     2041 tcgcgcaagg tggcaaacag ctggccggtg ctgcccagcg ccagcaggtg cgtcacgccc
     2101 gcctgccaca ccgtgggggc caggaagccg ccctgatccg cgttcgtggc cgtgccttcg
     2161 gacaggctgg cgctggcggc acgcagggcg tcgccccggt cgtaggggcc gccgcccatg
     2221 tcggccacgc tgccgtgggc ggcctgcagc cagacctgct gcacgttggc ggcggcggga
     2281 aaggcaatgc gcccgcgccg tacgtccagc gccgccgcgc gcggaggcac gggcagtgtc
     2341 acggcgttgg gtatctcgca gatccacagg cgctcgcgcg gtatctccac cggcaggctc
     2401 tcgccggcca gctgcgcgaa cacgcgcagc ccgggctgct gcgccgtcat gaatacgggg
     2461 ggcggttcag ccatgccggc gcgcaggcgt tccagctcgg catgcagggc cagcgagcgc
     2521 agtggggcgc tgacgttgtc ctcgcgcgcg gcctgggcag cctgggtcag ggcgcccccg
     2581 gtcaaggtgc gcgggcggtt gaacagcggt gcgtccacgc cggcggggtg catggcccac
     2641 cagcccgcgc cgctatcgcg tgcgctgatg aagtcggcat ccgcctcgcc cggcacgcct
     2701 tggcccagcg gctgggcgcg caggcgccac aggaagatgc cgatgttggg gatgttgtgg
     2761 cggccgccgc gtgtgtctgc gttgcgcact tccagggtgt gcgcgtatgg atcgaaggcg
     2821 ccggcgtggg cctgggccag ctcggcgcgt gcggcgtcgc gcacgccggc ggtggctgct
     2881 ggagcgcggc gcacatggtt catgtgctgc gtggtggcca ggcgctggaa aaattccacg
     2941 gccgtggccg gccagcccgt cacgtcgcgc gccagctgct ccagcaccag ggccgtgccc
     3001 ttgcggcgcc ggtacgccag ggtgttggcc acccaggcgc gcatgctgac gccggccgag
     3061 ggcacggggc ggatcgcccg tgcgccgacc aggtcgccga tgtagggcac ggtccactcg
     3121 tcgcaggtct ctatgaacca gttgtcgtac aggcgggcgg catccagctc gagtgccgtg
     3181 agttcttcct cgatcaccgc tagcagcgcg cgcagcggct ggccctgctc ggcgtcgcgc
     3241 aggcggtgta tggccggcag cagctggtac agcctttcgg gggtgagcag gctcatggca
     3301 tggcctccag cagttgcacg gcggcggggt tgatcagcag cagctcggcc ggctgcatgg
     3361 cgccggcgac ccagcgtgcg cccaaggccg gcagtacctc ttgcacggca tcggggctgg
     3421 cgccctggcc gtaaggctgc agcaccgaca gatccacggc gaccacgccc gacacctgtt
     3481 gcagcagggt cagcaaggcg gcggcggtca cgggttgggc cagctcgcgt gcgtcaaagc
     3541 cataggcctc gagcaggcgc gccaggcagt cggccagtac ggcggtagcc tggtagcgcc
     3601 catcgacgac cacgctggcc tggcagcgga agtagcgcaa cgccgcagcc tgtagcgtga
     3661 aggcttgcgc ggggtcggac tggtcggtga tggccaggcg caggttgtca agtacctggg
     3721 cgtcggccgt gccaccggtg gcgcccgtta ccgacagcag cacgcgcgtg ctggcgtcca
     3781 cgctgaccag gtcggcgcgt gccttgccta tgccgggaaa ggcgcgcgcg taatcctggt
     3841 agtcgcgcag cgagaccacg cgctcgaagg ccagcagcgt cagcggtgcg ttgcggcgtg
     3901 cgtcggccag tttctcggga tcctgcgcgc cgccggccgg cagcgggttg ttgacgccgc
     3961 gcaggcccag cggcatggcg cgcggcatgg tcaggctggc ggccgccacc tcgccatcgg
     4021 cacccagtcc ggtgcggtac ctggcgcgca cattcatctg cccggtgggc aggcgcgcac
     4081 cctggcggcc gtcgccgaac agcagggtca tgcgcgcgtc gttgtcgata cgcgtggcaa
     4141 acacatgctc gttcggtccc gcgccataca gcgatgggcg ttcgctccac aacaggccgt
     4201 tgacgcgcag ggccagcgtg ctttgcgcgc cgctgctgct ggcggccgac aggtgggtgg
     4261 tgggcgggcg gcgcagcgtg aactgctgga acggttggga ggcgtcgccg ttgcccagta
     4321 cctcctgcac gccctcgcca tgggtggcgg cgaccacgtt ggcgtgtatg cgcaggccct
     4381 cgcgctgata gctgaactgc aggccactct tgcccctgag caccagggtg ctgcggccgt
     4441 tggcatgcac cacgtcgtcg agtaccgcga tctcggccgc atccacgccg gggatgtcgc
     4501 tgcgcgcgcc gacaaaggcc agcagcttgc ccggggtgag gcccagtacc atggtggaca
     4561 gctccaggcg ctggctgccc gccgcgaccg gggcatcgat gggtagatcc accagcggct
     4621 ggcgctggct ggccacatgg gccgtggtgt cgcggaactt gaaggcttcc gaagggggtg
     4681 ccgacagtgg cttgccgtcg ctgccggcca tgcgcaaggc catggcgcgg ccggacaggc
     4741 cgtagtcggc gcgtgcgcac tcccggctgt cgtacagcag ataggtgctg gccttgacac
     4801 cgggcgaatc gaacaccatc cagctgcccg cggccagggc cggcacgggc cgttccaggt
     4861 aggtgtgctg gggtgtgatg gcgccgcttt gcgagtctgt ccagatgctg gggttggtgc
     4921 tgtcccagtt ggctgggtag taggtcgcgc cgcccttggt gttgctggcc ggcagggagt
     4981 tccattgcgg cgcgttgtgg ccgaaaaatc ccaggcgtgc gccaaagctg aaggcgccgg
     5041 cttcgggcgc caccggcttg gtggctggct gggcgctggc ggtgatggcc tgcaccagtt
     5101 gctggccgct ccagccctgg atggcgatca tggcctgcag ttcgccctcc tgccaggcct
     5161 tgccgctgaa ggtggtgctg atgctgccgc tggtgaaggg cacgctctga acctgcgcct
     5221 gggcgtaggg accggagatg gcgtacatga ccggtatggc ccattgcagc atgggtggcg
     5281 cggccggcgc gacggggtcg ggcagggggt cgagatcgat gcgcacacgc tccagcgtct
     5341 tttcttcttc gacttcgcgt gcgcgcagca ccagatggag caggttggcg ccgttcttgc
     5401 ccacgaacag caatagctcg ccggcctgta tgcggttggc gttcgcgccc aggtaaatgc
     5461 gacccacctc gagtgcatcg acgctggcat ccaccggcag acccggatcc aggcggtaca
     5521 ggctgccgct ggccaggccc tggtgcaggc cggcggtgcc cgcgggcagg ctgccctggg
     5581 gcagcagcaa caccagccgc cgtgcaccac cgctgacatt cagcaaggcc atgtcggccg
     5641 gccgtgtcag gcgcgggcgc agcgcattcc attcggcgcg cgccagcagc tcggccgagg
     5701 tctcgaacac ctggggcagc ttgccctgcg gcggcacgct ctggatcggg ctgccggcgg
     5761 cgatggtgca caggccgggc gcgcctggcg cgtcctccac ggtgaacacc agatgcacgc
     5821 cagcggcgac gccggggcgc agctcgtagc ccacggcgcg cgccagctcc agcaccgaac
     5881 ggcgctcggt ggcggtgcgc agaaagccct cgttggcgat gcgctcctgg tagaagctca
     5941 gcacatcggc cgcgcaggcg gcggcgtcca ccagggccac ggtggcatca acgggctcgc
     6001 gcgtggccag gtgcgccagg gggcgcggtg cgcctgcgcc cgcctccggg tcgcgccaca
     6061 gcggcagcct ggccagcatg cgttggtaaa agcccggctg ggtgtccagg cgccaggcga
     6121 gcgcgggcag gccggggtcg ttgtagatgg cagggcgcgg ctgctggcct tcgcagcagt
     6181 ggcagccgct cagatggtcg tcgctcatgg ctggcctccc attgcgtgta cctcgaatgc
     6241 gagcctgccg ttctcgggtg cattcgggtc gttgtcgagc cgcgcgatct ccagccgcgc
     6301 catggtgatc tggcctgccg ttatctcgcc cgcgggcgcg cggcccaggc gctgaaagcg
     6361 cagcggctca acgtgcgaca cgccggtcac ggccatggcg gcggcgacca cggcgcttaa
     6421 gtaaaccggg tcgccaaagc tgaagcggtc ggggtcgaag aagccgccct gcgcgctgcc
     6481 gaacacctgc agcagccgcc gctccacgtc ggccgtgaag tagccgggca gggcgcagac
     6541 atgcagcgcg atgtccagcg ccacataggc cggggcgtct atttccacgt cgtggccggc
     6601 caggcggtag cggtcgatga aggccgtcat gctgtcctcg aagggcgcgt ccacgggcgc
     6661 cccgccacgc cgatccacgg tgatgaacat cgtgtaccag ctacccgtcc agcgccgcgt
     6721 ggccacggcg cgctgcacgc gcgtgtcgcg catggccatg tcggcatagt tctggggcgt
     6781 aacggcgcgc tcctggcggc ggaaggcctg gggcgcgtcc atgcgcgcgc gcgccagcgg
     6841 tgtcgggccg gtgccgccgc gggcgggcag cgggttgcgc acgcgcgtga tgccgtcgaa
     6901 gccgcacagc acatgggcga tggcgttgct gcccacgttg ccgttgctgc cgttgccgcc
     6961 gcgcatgcgc agcgacaagc cctcggtggc gcccggcgag cggccgttga cgccgtcgcc
     7021 gaagcgcagc agcacgctgg cgtcgttgtc ggtctcgacc acgtagtcgg ctgcctgccc
     7081 gttgctgccc agcaggtcgg gctgcacgct ccagagccgg ccgtcaccgt cgctggtgac
     7141 acgcatggcc gcgcgctgcc cggccgggtc ggtctgcatg gcggctgcgg cgggggccgt
     7201 ggcatcgacc agcgtcaagc gtcccaacgc gtcgcgcacc atggcctggc gcgtgagggg
     7261 aagttggctg ccatgctctg gccgcaggcg tggcacgcgc ccgccgggca cgggcagcag
     7321 cttttgtgtg gcgctggggc tcatgccgtg gtcggccagc gccaggttgg cgcaggcgcc
     7381 ggccatgtcc tgtaccaggg agccgcctat gaccttggac aggcacaggt cgaatggcag
     7441 ggcgtcttcc tcggcccatt gcagttccac gatgggctgc agcgtgactg ggtcgcgccg
     7501 cggcgtgggt gcgggctggc gggcggcgtc agcctcgggg tctacccgcg tgagccgcac
     7561 cacatggcgc tgcgtggggt cggcgtcctg gggcaggccg ttgctggtgc cggcgcgcgc
     7621 ctccagcagc agcaggtcac cggcgcgcag gcgcaggcgc tgggccacag cgtcgcgcac
     7681 gaaggcgcgc gtggccccct tgggcaggca gcaggcctcg tcgctccagg tgtagaagcg
     7741 caggtcgttg tgcgcgctgt acagggtgac gggcgtcagc agctcgaagg gctgggcgcc
     7801 gccagccacc aactcggcgg cccgggcgcg cgatggcagg cgcttggccg tgccggccga
     7861 ctgggtcagc agcagcgtgc cgtccaggcc ggtgtccggg tcgcagcccg ccagctgctg
     7921 gccgtcggcc gtggcatcga cctcgaaggc gacccaggcg cgggcgttgc cgccctcggc
     7981 cagctggtag tccagcagcc gtgtgtggcg cttgaccgag acgcgctggc gtgctgtgcc
     8041 caggtaggcc tcggtggcca cggcgtcctg gaagtaggcg atctcgtcgg cgcgatacgc
     8101 cagggcctcg ctcagtgtca ccagcaggtc ggcagggttg cgttcgcgcc agtctggcat
     8161 gagcaccgac aggcgatcga gcaccaggcg gcggaaagtc gggtagtcgc gtgccaggta
     8221 gtcgatgtcg ggcaccgctt gggtctctgg cgggcagtcc cgggtctggc ggcagtcgaa
     8281 gtcgctgggg cagtcgacct tgaagccaaa gctgatctgc gccagggccg ggtctatgcc
     8341 gtcgggtggt gcgtccgcgc cggccgaggc caccagacgc agctggtagc gcgagaagtc
     8401 gccgctgtcg tccagctcca ccgtcagttg cttgccgctg gcgctcacgc ccaccacatg
     8461 cgggtcacgc acacgctggc cgccgcgcac ttcgacgttg cccgcggtca gtggtgcaac
     8521 cggggtctgg ctcaggtcat gcacgaagtg cagcaccaga cgcggttcgc cgtggatcac
     8581 ctccaggtac tcgatgccgt tgaaccagcg cggcggcaac tcttgcgcgg cggcgcgcag
     8641 ggcggcaatg cggcgcggat tcttgcagag gtattgctgc gtgctcatga tgcgaggctc
     8701 cgcgtgaact gggcctcacc ggcctgctgc gtctcgagca ccgtgtagcg cacggccacc
     8761 gtgagcgtgg cgtcctcact gctggcggcc acctcgtcga tgcgcagcaa ctgccccagc
     8821 cactgctgca gcgaggcctg aaccagggcc tgcacggtga cggccagctc ggggctgttg
     8881 ggggcgaaca cgagctgcat cagcccgcag ccgaagtcgg gccggttcac gcgctcgccg
     8941 ggcagggtga acaacagctg ctcgatcagg ccatgcagcc aggcctcgcg cgcggcttca
     9001 tgcgtgcggc cgcgcccgtc ggtgcggtag gggaaggcga cgtagctgct catgatgcac
     9061 ctccttcaca tcccaatgac gcgggtttgc gcggccaccg gcatcagcgg cgtgcccgtg
     9121 ggcgtgcaga tggcctggcc gctcatcacc agcaccggct ggccggcggc gaatacgcgc
     9181 gtggccgcca ctacccattg cgccgtgacg cagggcccgt tgccctgagg cggagagaac
     9241 gcacagcccg ccaccagcca gggcgcggcc tgcgtggcca cgggctggcc cgagaccagc
     9301 acgcgcggca cgggcgcgct gggcgtggcc tggccgccgt gggcgcagag cacggtggcg
     9361 ccgaggtgca gcagggggcc gggcatacga tgttccttaa acgaccgtca acgcgccggc
     9421 gttgatgttc gtcgtgggcc ccgtcatgat cagcgaggca cccttgccgt tctggatgta
     9481 gatgcccgtg tcgttcacga tcagcgtggc gccggtggcg ctcttgagca tgatgccgcc
     9541 ggtcgggccg ggcaggtcgc tgaccgtgag gccgttctgc agcgtggtct gcagcgtgat
     9601 cgcctgcact ccgggcggcg tggcgcgcgc cagcgctggc acctcggccg cgctgcccca
     9661 gaagcagccg acccagatgg gatagtccgg gtcgccctgc tcgaactcta cccacacgcc
     9721 tgagccgatc atgggcaggg cgaacatgcc gttctgcacg cccgccacgg gcacgcaggg
     9781 catggcccag gtggcgggca gcagcccggc cacgtcgggc acctgcacct gcagccggcc
     9841 gatctgcatg gggtcgatgt tgttgagcac catgcctcgg tacttgccgt agaacttgtc
     9901 gttcatcgtc tgttcctcct tcagaccggg acattgggag tgatcgacac caggccattg
     9961 cgcgtgagct cgaagctctg cttgaactcc ccgcgcttga gccggctggt cacgctgctg
    10021 acgtagtaca ggccgtcgta ggccacgccg gcgccacgca cgcccaccag gccgcgcgcc
    10081 ttcagtaccc ggccgtagcg cagcacgttc aggctgccgc tgccgctcac ggcgtcctgc
    10141 gacttggcgg cctcggccag gccggccgag atggcacgca tggggctcat cttggccgtg
    10201 tccttgagca ccttgaggtt ggcaatcggc gtaggcaaag cgcccagcgg cggctgcagc
    10261 gggttcaggt tcgggatggg gatcggtatc ggaacgcgcg tcagctggtt ctggatgtag
    10321 acgatgggca gcacgccctt ggtcggatcg aaggagaacg acagcgactc cacattggtc
    10381 agcgcgtcca tgtccaggtt cagtgccggc tgcggtacgc ccagcttgat ctcgggcccg
    10441 aagtaggcga tgttggtgcc cggggccggg cccggctcga cgtagaacac atagcccacc
    10501 tgtcgcgcca gctcctggat ataggccaga tctgtgccct tttgcgcggg aatcttgtcg
    10561 atggggatct gcacgtcggg gaacagcacc gggatcacca tggggatcag gccgaagggc
    10621 gcgtacttgg cgcacagcag ggccacgcgg ccctccaccg gcacggcggg aaatggcagg
    10681 ccgctgaaat cctgcatgtc catggccttg gtcacgtcct cgccggtcac ggtgagcgtg
    10741 ccggcctgac cacgcccgcc aggctgcacc tccacatggg tcatcacgcc gtcgaacagc
    10801 ggctgggcgc tgccgttcat ggtgaggatc agcagcacgc gcagcggcgg cgtggccatg
    10861 ccggtgttgg tgcctgccgc cagcaggaag atggtgttga gcggcgagcg cgcggtgatc
    10921 gtgaacgtga gctgaaagcc cgaggcgctg cccgccgccg tgcgcacctc cacgctctgt
    10981 agcgcatcca gcaccacgcg cggcaccggc agcggcacca gcgggccgac gagcagggtg
    11041 agctgtatgc cctgggcgaa catgatcagg cctcgaaggg gggcggcggc acgccctcgg
    11101 gcagggtgat gcgcaggctt gaacccggca cggccgtcat ggcctcgggc tgcatggcgc
    11161 cgttggcgtc ggccaggcgc caccattgct cggccacgcc caggtattgc gccgccagct
    11221 gatccgggcg ctggccggcg gccagggtca gggtctggat ggtggcgaag ttctccggcg
    11281 gcggcagaaa gcgccgcgtg acataggcca cggtgcggcc gtcggccagc tgggtctgcg
    11341 ccgtcggcag gccgtggtag cggctgtccg gcgcaaaggc tggctgggcc aggccgttgg
    11401 cctgcataaa ggcggcaatg gggtcggtca tggtcacact cccgtcacgc ccagcgtgga
    11461 cagcgacacc ggctgggcct tggcggccag ctgctcgcgg ttttgcagat aggtcatgaa
    11521 cagcgcgccg cccttggagg caaagcccag gtcatcgacg gtgagcacgc gcagaccgaa
    11581 gctggccttg gcgtgtatcg ggttgagcgc ggggtcgaag gcctcctcgg tcaccgagaa
    11641 gctggtcagc ttgaccggta tcacgcggct agcgccccag accagcagca gcagcggcgc
    11701 cagcatgggc gcgatctcca gctggcccga gctggcctgg ctgttcacct tgctcaactg
    11761 cgcgctgctg ggctgcgaca gcgattccag cagcgcgatc tgcggcgcca ggccatgggc
    11821 cacggtggcg gcatgctggt ccgggaattc gagctggtcg gtcgcatcca ggtcggcctc
    11881 gaagctgatg gtctccaccg ccgggccctt gaagcgtgtg ggctcggcac gatcgccccc
    11941 ggcctcgccg ccgacatcct ggcccttgag ctcgcgcttg agcgattcag cgttgtattg
    12001 cagcgcgatc acgcgttgca cctgggcggt ggtgggatcg acgagcacga tgccgccctt
    12061 gatcagtttg ggggagaagg gggtggtggt catggcggac tccaatggat ttcagccggg
    12121 ctcgaccagg cgccagctgt tgcaagtccc gggcaggttg tcctgaccgt agcgttccat
    12181 gatgcgcacg aagggcgcgt agtcgcgctt agacaccaca gagccgtagc ccatgatgtc
    12241 cgtcttctgt tcggccgtga tgccgtagca gcgttcggcg ctgccgtcgc agaccacgtg
    12301 gctcagcccc atcaggtggc cgaattcgtg cgccaccgtg gtttgccgga aggtggccat
    12361 gcgcaggttc ttctccgagg gcggtttctt cggatcaatc agcaccggcg actggcgctc
    12421 tttcatcgcc gtgtcgttct cctgccattc ggtgctcttg ctgcgctcgc ccttgtcgga
    12481 ccagaggttg atttcgcggt gcggtgcttt gctggtgaac accacgagca cctgcgcctc
    12541 gaacttttca tggtcgccgt ggcaggccga ctgcagcgcg tagatgcccg accaggtgga
    12601 ttcgatcacg gcctgggcgc gggcactgaa tctggcgatg cgctcggcct tgtcttgcgg
    12661 cggccccatg tcggtgagct tgagccgcat gatcagccgc acccggtggt tgcgggtgtc
    12721 caccgctgcg tcgaagcggc caccgaactc ggtactctcg taatagaaat gctgctgcgc
    12781 ggggctgctg gcctcgggtt tgcgcatcag gcgcgcctgc tgtggtgccg ctcgctgctg
    12841 taccacatgc gccaactcgt gcgccagcag gcgcctgccc tcactggtgg tgggtgcgta
    12901 ttgattggct ccgaacacaa tgtcgcgacc cagcgtgtag gcgcgtgcat gcaccgcccg
    12961 ggccgaggcg gcagcaccgt tgccggcgtg gatgcgtacc tggccgaagt cgtgaccgaa
    13021 gcgcgactcc atgaagccgc gcgtgccctt gtccagcggc gttccggcac tcgccagggt
    13081 ggactgcacg gcggcgtagg ccgtgggagc catcaccgtg cgcccgtcaa ccgccgcgcg
    13141 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacggcggc gtaggccgtg
    13261 ggagccatca ccgtgcgccc gtcaaccgcc gcgcgctgca gtcgctcggg cccgctgggt
    13321 gcggacgggc tgaagtactg gaccagcttg ggtacgtcca gcagcaccat gaagcgcaca
    13381 tcgcgctggt tggcgtagag gtccggcagg gccggtgact gcgccatcac gcgcgagctg
    13441 tcgccgcccg gaccgcccgg gccgggcagc gggtagggcg agatgccatt gcgaaaaccg
    13501 cccgacagct gcagcccatc gccgaatagg ccaacctgca cctgcagcgc agacagcgaa
    13561 acgggacgcg ggccggccgt gtccagcgtg ccgccggtgt cgagcttcag gctggagacg
    13621 cgagtgttga gcacgccctg gttgaaggcc tccatgagct tcgaagcatc cagcccgatc
    13681 tcgaaggcga agcggctttg cttgccgtcc aggatctggt acttgaaggt cttgaccgga
    13741 aactgcggca cgatgcccgg cagcagcgcc aggttgaagt cgtcgaactg ccggcgcatg
    13801 gcagggtcgg ccagcgccat ggtccagaga aaggcgtagt tcgcgcccag gaaaaccggc
    13861 acgccaatgc tcagcggcgc cggctgggcc atgaagcggt cacccagccg ctcggtgaat
    13921 ttgctgaagg cggggttgtt ctcgcccagg ttgctggcca ccgtgctcag gccttcggtc
    13981 accgggctgg gcggcggttt ctttttctcg tcctcgccct cgcgctgcag gggctgcgat
    14041 gccaatcgcg acagctgggg cctgccgccc gcggccagca cctggtctgc cgcctggtcg
    14101 gcctctcgct cccagcggtc gcccggtgtg ctgacggcca gcttgggctg cgggcccggt
    14161 atgttccggc gcgcacaggc cgcgcagcgg cctgcggcag cgccgccgca ggcgcaccgg
    14221 ggttgcagcc tggcgtacgc gttcatggct tgccgccctt cttgggtgcg gccttcgatc
    14281 cgccgccggg cacctgcacg gtcaccacgt tggcatccgg cgtctggccc gacaggcagt
    14341 gcacgtagtt ggcccaggag tcggccgatt cgaaggcgcc gggcgaatcg cagtcgaaca
    14401 ccgggtagta ggcctcgccc gcgtccgcgc tgccgatgcg ctgcaggtgc gccatctcgt
    14461 gcaccatggt gcggatgcgg ctttccggcg tcgtgccctt ggcgaagaag gccgggcaca
    14521 gcacgatggg tgcgcggtag ccgcgtacat agccggcgcg ggtgccgcac tccgggtcgc
    14581 ccgggccggc gaccttggcc tcggccgtga tcacgccgcc gcccatgccg cgcagcacgt
    14641 cactgatctg ctccatgttg gggtccggcc agtcaaactt gatgcgcgcc agggccttgg
    14701 cgcgcatcag cgcctcggcg ccctcgatgc cgctggcgcg gaacatcgcc agctgcgtgc
    14761 gcacggccgc gccgcgccgg gcgtcgtcga tcatcttctg ctgcgtctga ttcggcgcga
    14821 ccggcggcac gaccctgggc gcgggtgtgg gtttgtcctt gccatcgcgc tgcacgccca
    14881 gcgggtggcc cgcaccttgc tgcaccacat gcgccagctc gtgcgccagc aggcgccggc
    14941 cctcacaggt tcgtggcgca tagcggccgg gcgcgaatgc gatatgcgcg cctgcggtgt
    15001 aggcgttggc gtccagttcc tgggtcgaac gtgccgcagg cgcatcggta tgtacccgca
    15061 cgcgcgaaaa gtcgtggccc aggcggcgtt ccatgtcctg gcgtacctcg ggctccagcg
    15121 cccggcccgg cgcggccagc gtcgtctcca cgcttggcgg gctggcgtcg ggcgtcattg
    15181 acgcggcgcc cggcgcggcg gacaggcgct ggatgcgcgg catgccggct tgctgcagcg
    15241 ccgggccgcc ggccacctgc tgcgccacgc gctcggcttc gcgttcgtag acgtcgtcgg
    15301 ccgcgcccac ggccagctgg gtctgccagc ccagcgtctt cctgcgctgg caatcggcgc
    15361 acgcgccgga tgagccgacc ttgccgccgc aggcgcattt gcgctgcagc cagagacccg
    15421 tgggggtgct ggcggacacc cgtgcatgtt gcgggatggt gttcatttcg cttcaccccc
    15481 gcgccgtggc gccccacctg cgataccgcc tgcgaccgcg cgacccagct cggccgcggc
    15541 agtgcccggg cgcagccgaa cgggtgcgac atgcagtacc gccgctccgt catgcggcgt
    15601 cagctgccgt gcgacgtcgc cgacgccgag ctgtgtgcgc aactcggcct ggagggccgc
    15661 ctgcaacgcg gccggatcca cgtggtcaac gccgcgcagc accagtcggt cgatgtgcac
    15721 cacgatgcgg ttcatgagtc ctccagcctg gcccaggaca cgcggtcttc aaacgcgaga
    15781 tagaaatccg ggccaaattc ccgcggcgcg gccagcggtg ccatgcctcg gcagcgcgcg
    15841 atatgccgct cattgccctg cagggactgc gccacggtca gcaccaggcc gcgcgtgcag
    15901 tacagatatt cgtgcgcata gtgcccgggc accagaatct cgtgcgcgag caccgcgtcg
    15961 ggtgcaggca gcgtatcaag caccgcgggt gacggcaggt gcgccggggc aagcatcacg
    16021 gcctggccgc cgcgcgacca catggccagg gtcccgtcgt cgccggccgc gtagtcgtgg
    16081 cgcattgccg gtagggcacc caacggctct tcgcgtcgca ccgtgggcgg gccgtacaga
    16141 gcgtcgaagt cggccaccga caccgctggc aggccctgcc aggcggcaag atcgccggcc
    16201 agcagcggct gcagcaggat gcgcagggac tgcgggctca tgtgatcccc gccagttctg
    16261 gccggcccag gaagtaggcg gcgcgaaaac ggttatcgcc cacgcgcgcc cggatggcct
    16321 cggcgccggc gccggccgcg ccgtagccga tggcatcggc cgccggtgcg gggcagggcg
    16381 cgccgctgac gccggcctcc agctgttgct tgaacgccgc ggtcgaagcc gccttgggcc
    16441 gcacgcgctg gttgaagagc tgcgcgccca gcacttcggt gaagccctcg cggatgattt
    16501 gtggaaagcg caccgtggcg gccgccgcat tgaagcccgg atgcaccagc gcgtgcagca
    16561 cctcgtggca cagggttgcg atgccgcgcc agtgatcggt gcatgcgctc gcggtgtccg
    16621 cattgaactc ggtgaccagg cctatggtcg tggccgcgcc ggtgccggac ttgcggccgg
    16681 tatgctggat cagccggtct acggcgtcgg cgatggcctt ttggccctcc agggcggaca
    16741 ccacggccag cagctcgcgc cggtcgtcgg cgcgtgatcc atcgaaatgg gtctggtcga
    16801 agatgttggc gtcgttgacg atcgtcgtga ccgcagtgct gcggcccacg atttcggcgc
    16861 ggttgagcag atagccgatg cgctgatcct gcttcggcgc cagggcaccg acatcgaaga
    16921 tgtgggccga ggcgtgccag gcgggctgca ggtcgtacag gttgccaatg gcggcgtcgg
    16981 cgtagggcgc gaagaagctg cgcgcctcct gctgcacgat atcgccgagc gcctgaaagg
    17041 cggacagcga acgcgtgccg cgcttggtcc agcgcgccgg atcgagctgc tgcaccagat
    17101 gcttcagcgc cggcggcagg cggcgcttga attcgcccac ctccagcggg tcggtggcgc
    17161 tgccgctcga aggcggggga ttgacgatct tgtcgaccgc ggtgcggtca gcggcccctg
    17221 gaaccggcag gcgctcgatc tgtgcggtct gcgaccagcc ggattcgcgc agcacctcgc
    17281 gcacgaactc gttgtcgggg tcaggcagcg gccggtgccg cgccgagggg gcgtaggcga
    17341 gcagccgatc cacagcggtg ctgccgctca gcgtgctggc ggcggcgcgg ttctggctca
    17401 gcaattgcag cgcgaacagc agccagtcct gtgccgcgat gcccagagcg cgcgcggtgc
    17461 tctcctgcgt ggcagagggc ggtgcaccga tcagcgtggc catcagcgcg acgacgcggt
    17521 cgggtcgatt ggccgccgtg acaaagatga tgggaccgta gtcgatgtgc acgcgcacgg
    17581 cgccctggcg catctcggca cgtcgcagcg tcacggccgg tgcggccgac tgctgcacca
    17641 catgggccag ttcgtgtgcc agcaggcggc ggccagcggc actgcgcggt cgatactggc
    17701 cggcatcgaa cacgatgtgc cggccgacgg tataggccag cgccgccacc tcgcgcgccg
    17761 acagtgcggc catcgcgcca tggtgcacgc gcacggagcc gaaatcgtgt ccgaagcgct
    17821 cgccgaactc ggcgcgcgtg gctgcgtcca ggggcatgcc tggctcgcgc aacgtggcct
    17881 gcacgctctc gggtgcgggt gagcccgcgc aagcgcccgg tgccgcgcgg cgcgacagcc
    17941 gctgcgccgc gggcgcgcca ccctgcggca gcggcaggtt ctgcagcgcg cgttcggcgg
    18001 ctcggtcggc ctcgtcctcc agcgggtcgt cggccgcgcc gatggcgagc ttgcgttgca
    18061 ggccgaacgc gcgacgctgg cgaccctcaa aacgttctgc tggctcgcct gcctggtcgc
    18121 agccgcactt gcgccgctgc aagcgcatga cgtcgcggtt cgccgtgtcg gcaacgcgtt
    18181 gcgtggcggt ggtgcgcacg ctcatgtcca ccccctcagc tccgcctcgg aaaacggccg
    18241 ctcgcgcttg gcgccttcca tgcgcgcggc ctgctgcagc tgcgccatgc cgatgtcgct
    18301 gccggcctcg gcggcgagga aggcggcagc cagcgcgatg ttgcggatgc tgccgccggc
    18361 cacgtccagc cgtgccagcc gctcgaaatc catgtccgac gccagcggcg cggccttggg
    18421 aaacacggcg cgccagatgc cttcgcgctg cgcctggtcc ggaaacggga aatgcacgac
    18481 gaagcgcagc cggcgctgga aggccgggtc cagcgccgcc ttgtggttgc tggtgaggat
    18541 ggccaggccg ccataggcct ccatgcgctg cagcaggtag ctgacctcga tgttggcgta
    18601 gcggtcgtgg ctgtccttga cctcgctgcg tttgccgaac agggcgtcgg cctcgtcgaa
    18661 cagcagcacg gcgccggcct gctcggcggc atcgaacacg tggcgcaggt tcttctcggt
    18721 ttcgccgatg tacttgctga tcacggccga cagatcgacg cgcagcagtg ccaggtccag
    18781 ctcggtcgcc atgacctcgg cggccagcgt cttgccggtg ccgctgtcgc cccaaaacag
    18841 cgtggccagg cccagcccac gcgcgccctg gtcggcaaag ccccagcggt ggtgcacggt
    18901 gagccggtgg cgcgcgtgcg cggcgatctg gtgcagcgtc tgcagctgcg ccgggggcag
    18961 caccagctga ttccagccgg cgcatgcgtc cacctgctgc agcaggccgg gcaggaccgc
    19021 gctgcggccg gcacgttcac gcgccgcgcg gcacagcgtg gccgtgtcgt cggcctggcc
    19081 cgcggcatgc gcctcggcgg caatctcggc aatgcggcgc gcgcccaggc ggaactgact
    19141 ggccaggccg tccagcgcgg cgggcggcag ccgcgcggcc cggtgcgggc ccagcgcctg
    19201 ctgccacagc cggcggcgct cgggtgcttc gggaggatcc aggcgcaggc gatggcatgg
    19261 gaactcggtc gccggccagg tgcgcccgct cagcagcacc aggccgggca ggcgttcggc
    19321 caggcgcaga gcggcggccg gttgctcggc gcccgccgcg tcctcgcact ggatgagcag
    19381 tccggcgccg agcagcaccg cgtcgcggct ccagagggcg gcgaattggg cctgttcggc
    19441 ggcaagcatg ggcagatcga agccttgcag gcagtacagc cgcgcgtcca gttcggccgc
    19501 cagggcagct gccaggtcgc gctggccgcc ggcatcgtca ccttcgagca ggatgaaccg
    19561 gggtcgttcc cgccatcgag cgagcaggcg cgctagcccg gcctgctggg cagcggccag
    19621 gggcggcgcg tcgtcggaca ccggccgcaa caggctttgc agccggtagt ccaggtggtc
    19681 cagcccggcc aggtaatgca gcacgcgttc ggcgattctc agccgcgcgc tggtcaggcc
    19741 cgcaccctcg tcgatttcca gcagccgcca gtggcgcagc ggtgcatcgg gcgcgagggc
    19801 gctccagtgc ggatcgggca gggcggccag cgccaggccg aagctggccc agggccgctg
    19861 cgccgtgccg gcctgcgcgc acagagcggc caggcgcgca tccatctcca cgccggcggc
    19921 cagcagcacg agctcgcgct cgaacggact gagctggaac agcgcggcca gggtgtcgat
    19981 ggcggccggt gccggcagcg cggtgcgggc cgacgccagc gccgcgttgc cgccttcacc
    20041 cggttcgccc agcagcgcgc gcagtcgggc gaactcggcc actagcagct gctggttggc
    20101 ggcggtccag tcggtgacgc gttccggggc gttcatgtga tgctcacggt gatgggcagg
    20161 aagaccggtg tgtcgccggg ctgcagatcc atgtcgacga tggggctctc gataccgtcc
    20221 acgcgcaggc gcgcgaggta ggtgccgggc acggcgttcg ccacgcggaa ctgcagcgag
    20281 gtcggcgtcg cgccggccgt cagcggcagc acctcgctgc cgcccagcac cagcgtggcg
    20341 tgctggcccg tgcgcagctc gggtgtgaag ttcagggtga cggtggccga gccatcgcct
    20401 gcgcgcgcca ccgtcgccgg caggttggtg atgcgcggtg ccagcgccag cgccagccgg
    20461 ttgctgctca tgcgcctggg ttggttcagc aggtcgggtt gggtggcctg cagcgtgact
    20521 tggtagatgc ccacgggcag ggcggccgcc tggccggcga gcgagacggc ggccgtggtc
    20581 gcggccggac gatcgggcgg cagggggggc tggacggcga gttccagcgc cacctgcaac
    20641 ggctcgttgt tcagcgtaac gcggcggtcg gcgccatcga gcgattggcc atgcagcacg
    20701 accaggtcgc ctatgccggc taccggctgg ccggccggcg gctgggcacg ggtcagcagc
    20761 ggcatgctgg cggccaggct gggttggacg gcaatgccgg cctcgcggcc ctgggcatcg
    20821 cgtgggccgc gcgtcagcac gggcagggcg ctgcgtgccg gccgctgcgc ctggatcagc
    20881 accaccgagg cggtataggc ggcgctgggt cgcaggtggc tttgtgtcgc ggtccagaac
    20941 ttggacatct cctcgctgct ttgcgtggcc ggattgatct tgatcagctc gacctgctcg
    21001 gccaggcccg aatccgccaa ggcgcgcagg gccggtggca gggcgttgcc cacgctgggt
    21061 gaggggatca gcgaggtgcg tatggcctcg cgcgtcagca ccggcgtctc gtgcagcagc
    21121 tgcatggcgt agcccagcaa tatctcggcg tgcaggtcgg cgccgctgta ggccgacagc
    21181 agatagtgca ggtcgagggc cagcggcggg ttggtcaggc gcgtgcggcc gctgccgtcg
    21241 cggcctggca ggccctcgtt gcgccagccg aggttgggcg tgaccatgta catgaacagg
    21301 ttgagctgcg aggcctcggg tgtgctgccg gtcaccacgc ggtcgggcgc ctgcacgctg
    21361 acggtgacgc tcgatcccag caagccactg atgttgtggt tgatcagccc gtcgttgagc
    21421 aggtcgcgca gcaccgcggt cacgccggcg atggccaggg cggtgctcat gaccgcccct
    21481 tgcgctgcgc gaggtaggcg tctagcgaca gcggctgggc gcgttcgcgc gccggacgcc
    21541 tgggcgcctg gggctcggga gtcaccgcag ccgtgacctc gatgcggcca atgtggatat
    21601 gaacctccgg tggctccgaa cgtggtggtg catgggccga cagctgcgcc agcccccgca
    21661 gtgcatgtgg tgcagcagct acctggggct gtgccttgcc cagtggcggg gaggatgcgg
    21721 ccggtgtgtt ggcggtgttg cgatcggcgt tgagcggcag cagcggcggc gggtcggcat
    21781 gcactgccaa gggtacggcc catggtgtcg ggacatttgc cgtggcagca gacggcgggg
    21841 tgttgggtgg cgtcgcgtgt ggcgcagctt gcgccagcgc tgcggttgcc acgctggctg
    21901 ccaacccgga tgggcgttgc tgcggcaacg gtgctctgtc ggccggtacg cgagtgctgg
    21961 ccggctgcgg ccggtctgtc gccatgttgg cggggtgcag ctgtgtgccg actggaggcg
    22021 caggtgctgg ccgggtcacg tgagggcgga tggcgcctgc cggcatgggt ggcgggtgcg
    22081 cggcggtggt cgtgccgggc gcgagcaagg tggcccctga cgggctggac gtgacaccgg
    22141 caaacggcag acttgcgtcg ctttgcagcg cccaggcgct gcccgtggcg cgcgcggcca
    22201 gacgttgcaa caggccggtc atgacatgcc ctccaccagc gccaggtact gcgcgcgccg
    22261 cgccggcgac agggccagga tctgtgcctc gctccagccg taggcgctgg ccaggcggtg
    22321 cacctcgtgc agcagggcgc ccgcgcgggc ggtgatttcg tcccacagca gcgcggcggt
    22381 gtccagctgc gcctggtctt cgtggccgca ggccacgcag ttcagggtga aggcgagatc
    22441 ggcctgcgga tccagcgctt ccagcgcatc ctcgacctgc gcgcgtgtag ctgcgtccag
    22501 ggggagcgcc cggccgttga gggtgcaacg tgccaatagc gcatcggccg ctgcatcagg
    22561 cgcaaggctg gcgactgccg ccagatcgcg caggctggaa gcacgcacct gcaagccgtc
    22621 ggcgacagtg accgtcggtg actcaggggc tgttccatcc tcgggtgcag caccgtgcag
    22681 cagctcggcc acctccagcg cgacggccag ccgttcaccg cattgttggc agtcggcatg
    22741 ggcttcgatg cgtgggccga aattggcggc gcgcaggcgc agcaggctgc tgcctatggc
    22801 acccagcggc aagtcggtga tggcgtcggc aggccaattg gggtgcgcgg tggcggcgag
    22861 cagggcggca cgatcgagcg cgtggcgcga catgccgcgt tcccacagcg acagcagttc
    22921 ggcggcgccc agaacgcgca tgacaagcac tccgcgatca cacgggttcc gtgaaggatg
    22981 gctcggacgg ttcgctcacg tcgtagtcgc gctcccagcc ctcgttctca agtttcaggt
    23041 gctgtatggc cacggcgttg gcgttggcgt ccaggtctgg cagcgcctgg tattcggata
    23101 cccagcagcg gaacaccttg taggccagcg ccagctggcc ggcctcgttg tagacctcga
    23161 tgatcagatc cttgcggaaa tccttgagcg aaacctcggc gcccaggccc gagccgaagt
    23221 tccagacctt gttggcccat ttctcgaact cggtatcgtg cgtcaccccg cgctccagcg
    23281 tgatggcctc gtacttggtg cggccggggg atttgcgtga ggtcgagggg tcgccgccct
    23341 cgcggtgctc caccggctcg gtgctgcgtt tcaaggcgcc gaccttggag atgccggcca
    23401 catatttgcc gtcccatttg acgcgaaact tgaagttctt gtacgggtcg aaacgggtgg
    23461 cgttgacgct gaactgtgcc atttcggttc tccttcgtca ggcctggatc tggccggcca
    23521 tctgctggat cttgatgacc acgaactcgg ccggcttgag cggcgcaaag ccgacgatca
    23581 cgttcagtac gcccaggttg atgtcggcct gcgtggtggt ttcgctgtcg cacttgacga
    23641 agtaggcctc gcgcggcgcc ttgccctgga aggccccctg gcggaacagc ccctgcatga
    23701 aggcgccgat gttgagccgc acctgggccc acagcggttc gtcgttgggc tcgaacacca
    23761 cccactgcgt gccgcggtac aggctttcct ccaggaacag tgccaggcgg cgcactggca
    23821 cgtacttgta ctcgtcggcc tgcgcatccg cgcccttgag cgtgcgtgcg ccccaggcca
    23881 cacggccata ggccgggaag ttgcgcagcg cgttcacacc cttggggttg atctgcccgt
    23941 tctccaggtc gttgagcggc gcggccagat ccagcacgcc gttgagcgtg gcgtccgtgc
    24001 ccgcgggggc cttccataca cctcggttgg catcggtgcg tgcatagatg ccagccagcg
    24061 tgcccgaggg cgccatggcg cgcgggcgca gttcgtccag cggatcgacc agcttcacgg
    24121 ccgggaagta caccgccgcg ttgcgcgact gcgtgacgcc attggcccag ctggcgatgt
    24181 tcgacaggtt tttgcccgca ggggcatcga cgatgtagaa ggcgcggcgc ttctcgcaca
    24241 gatcgaccgc cgcgggaatc acggccgcgg cctgcagatc ggtcatgtca taggtttcgg
    24301 ggatgctcaa aaggttgaac aggtcgacgt cctgcaacgc atgcatgccc gtcttgccgc
    24361 tggcgctgcc cacgatctcg ttggcaccgg cgcgcgtgcc atccacgccc ttggtggggg
    24421 gcaccaggtt ggcgacctcc gctgggcggt gctgggcgtt acccgtgaac tcgcgcccgc
    24481 cattggccag gcccagatgg atgctgcccg acaggcctcc aaaggcaccg ccggcaatca
    24541 ccacgccaga cgattcgccc gcggtcagcg atgccagctt gaggcagccg gcgccggcgc
    24601 tgccatcggc cagggactcg gatgcctgca gcagcgcggt caggccggcc gtgccgatgg
    24661 cggtgttcac cgcggcaacc agggcggcca tgctggcggg tgccggcccg gccagcgtca
    24721 gccggaaggc ctggctgcca tcgaccacgc cgccaatcac cagatccgtg gctgtgagcg
    24781 ggaacgtcac cgtcttgctg accgcgaagc ccgtctggtt gaaggtgagg ccgcccctgc
    24841 acttggcgct gaccagggcc gaggcgttgt tcaccaccga ctctacgtag cgtgccgagg
    24901 aggggttcat gtccaggttg cggtgtgtct ccaggggcac cagggtcacg ccgtcggcct
    24961 gcagctgcga gacgatgagg ttgaactggg cgtcggggtt gagcgcggcg gcacggtcca
    25021 cggtgatgcg cagatcgttg ccccaggccc ctggggaggc tgtcatcacg tccaggacgt
    25081 gggcggcggg cgagccgccg ttgaacgtcc atgcggccga ggcatagccg gtggccacgc
    25141 gcacgatgat ggcctgggcg ccaccgttgg caaaaaactg ccgcacgccg tacgagaccg
    25201 ggctgtcccg atcgagcccg ccgtaggcgc gctcgaaatc gctgaagctg gtgatcggaa
    25261 cggccttgtc gggcgttccc atgcgggtgt agccgacaaa ggcggtgatc gatgtggcaa
    25321 ccccggtcag ggtgcgtaca ccgctgggca gttcctctaa atagacccct ggataactga
    25381 gcgcactcgg catatatcct ccaatcggtc gatcgatgac tagcgcgcca tgcggctgcg
    25441 ctcatgccct ccggtgattt gcttttgtgt tccgcgctat tgctgcaagg taagtaaaca
    25501 ctggctttga ttctcttgag ccgtccatgc aagtcaatgg acagtttcat ttgctgacac
    25561 cacttggccg cgcttaacaa cctttgtcaa tttgtggtca aacgcttgag ctggtagagc
    25621 gcatccagcg cctcgcgtgg cgacagttgg tcggggttga tctgcgccag ggccgattcc
    25681 acggggctca ccgcctggat ctcgggtgcg ggcggcgggg cgaacaggtc cacctgcagg
    25741 tcatcctcgc ccgcgcgctc ctccagcgcc gccagcgtgt ggcgcgcgtg gttgagcacg
    25801 gcggcgggca cgccggccag tttggcgacc tggatgccgt agctgcggct ggccggaccg
    25861 gcctggattt cgtgcaggaa gacgatgtcg tgccccgcct cggtggctcc cacatgcacg
    25921 ttgaccgcat gcgcatggcg cgccgccagc tcggtcagct cgaagtaatg cgtggcgaac
    25981 agcgtgaagg cgcgggtctt gtcgtgcagg tgcgtggcaa tgccgctggc cagggccagg
    26041 ccgtcgaagg tgctggtgcc gcggccgatc tcgtccatca gcaccaggct gtgcggcgtg
    26101 gcgccgtgca gaatctgcgc ggcctcggtc atctccagca tgaaggtgga ctgcgcattc
    26161 gccaggtcgt cggccgcgcc tatgcgggtg tggatggcgt ctatcggccc caggcggcag
    26221 gcgctggccg gcacatggct gcccatgctg gccagcagca cgatgagggc cacctggcgc
    26281 atataggtcg atttgccgcc catgttgggg ccggtgatga tctgcatgcg ctggttggcg
    26341 ttcaggcgcg tgtggttggg gatgaaggcg ccgctcgatg tttcggctag ccggccttcg
    26401 accaccgggt ggcgcccggc ctctatgtct atgcagggct cgttcacgaa ctgcggcgcg
    26461 caccagttga gggtcagcga gcgctcggcc aggcagcaca gcacgtccag ctgcgccagc
    26521 gcctgcgcca ggcgcgtgag ccgcgccaca tggggctgga gctggtcgag cacctgctcg
    26581 tacagccatt tctcgcgtgc caggccccgc tcctgcgccg acagggcctt gtcctcgaag
    26641 gctttgagct cgggcgtgat gaagcgctcg gcgttcttca gcgtctggcg ccggcggtag
    26701 tcctccggca ccttgtccac atggctggcc gtgacctcga tgtaaaagcc atgcaccttg
    26761 ttgaactgca cgcgcaggtt ggggatggcg gtgcgcgcct tctcgcgcgt ttccagatcg
    26821 agcaggaagt cgtcgcagtt gttctgtatg gcgcgcagct cgtccagttc ggcatcgaac
    26881 cccggggcga tcacgccgcc gtcgcgcacc agggcggcgg gttcctccag gatggcggcg
    26941 tgcagcaggg cggtgcagtc ggcgggcggc tgcagctcgg caaaaatttg agccaaatat
    27001 ggctctggcg cttgactggt aagcgctaaa agctctgctt tttgtagtgt cttgcacagc
    27061 gccaccagtt cgcgcgggcg cacctggcgc agcgcgatgc gggccgtgat gcgctccacg
    27121 tcgctcacgc ccttgagttc gccgcgcagc agctgccacg gcccctggcc gctgcccgcg
    27181 ccgcgcagaa cctgcgtggc ggccaggcgc tggcgcgcgg cggtgcgatt gcgctcgggc
    27241 tccagtagcc agttcttcag cagccggctg cccatgccgg tcatgcagct gtcgagcagc
    27301 gagaacagcg tcgggccgtc ttcgccgcgc agggtcttga ccagttccag gttgcgccgg
    27361 gtggccgccg gcaggtggat cagtgcgtcg ccctgctgca cgcgcacgcc gtgcacatgg
    27421 gtcagggtgc ggccctgggt gtgctcggcg tattgcagga gcgcggcggc ggcggcgtgg
    27481 gcatcggcca gaccctcggc gttccaggcc tgaaggctgg ccgcccccag gtgctccagc
    27541 agcttgcgct ggcctaaagc ggcgtcgaac tgccagtcgg gccgcaggtt catcgggcag
    27601 gcgaaggcgc cgccctggcg cagctggttc agctgctcct caaagcgctg cgtgatcccc
    27661 gcgctgtaga tcagctcgct gggcgcaatg cgcgccagcc aggtgcccag ctcggcctgc
    27721 gtgcattcgg ccagctgcac cacgccctgg gtgaccgaca gccaggccag gccgcagcgc
    27781 gcgcgcgcac cctggtgcac ggccagcagc atggattcgc ttttgtcggg cagcagctcg
    27841 ctgtcggtca gcgtgccggg cgtcaccacg cgcaccacct tgcgctccac cgggcctttg
    27901 ctggcgccca cctcgcccac ctgctcggca atcgccaccg attcgcccat cttgatcagg
    27961 cgcgccaggt agttctccag cgcatgaaac ggcacgccgg ccatggtgac gggttggccg
    28021 gccgactggc cgcgctgggt cagcgtgatg tccaacaggc gcgcggcctt ctcggcgtcg
    28081 gcgtagaaca gctcgtagaa atcgcccatg cggtagagca gcagggtgtc cggatgctgg
    28141 cttttcaggg ccaggtactg ggccatcatc ggcgtatggc cggagaaatc ggcctcggcg
    28201 gggcgcggcg cgggcgcggt tttgccggtc atctgctggg gtcgtccttg gggtgggcgc
    28261 gttgtccatg aaggaaggcg ccgggtatcg gtgctaggcg gactgggggc cggcgcggcc
    28321 atcttccacc acgaaacggg ccagagtgta cgcgtcgctg ccggcgtcca gcgggttgcg
    28381 cagggcgcgc agctgggtcg tccagtgttc tggcgtgatg tcgcacaggc cgtagccgcg
    28441 ctcctcgctg cgcgccagca gcacatgggg gttgctgcgg cgcagggcgt ccagcctgtc
    28501 ctgcgtggtg ccggcgcgcg agctgatcga ggtgccgcag aactcgctgg ccaggatgag
    28561 ccctgccttg cccgcctcgg gcggggccat caggttgcag acatagttct ggtggatgtc
    28621 gccacccagc accacggtgt tgcgcggggg gtgctgcgcc agggcgttca gcaggcgtgc
    28681 gcgtgcggcc gggtagccgt cccagctgtc ggcgggcagc tggcccgagg ggtagtggcg
    28741 cggcgagagc agggtctgct gcgccaccac gctccagcgc gcggcatgct cgcgtccatc
    28801 ctgggccagg ccctgttgca gccaggcctc ctgctgccag ccgagaaagc tgcgctgggg
    28861 ggctgccagc tcgctgcagg cgcccgggtg cacggagccg gccgaccggc tgccgggcgg
    28921 gcggcaggcc tgccggtcgc ggtactggcg cgcatccagc atgtgcagtc gggcgagccg
    28981 cccccaggcc aggcgccggt agagctgcag accctgaaag ctgctggata ccagggccga
    29041 tgcgcgcagc ggcatatgct cgtaataggc ctgccaggcg gcggcgcgca gcggtggcca
    29101 gcgggcgtca cccaggccgt ggttgccggc gtagtcgttc tgcacctcat ggtcgtccca
    29161 ggtcaccatc cagggcgcgc aggcatgggc ggcctgcagg tcgggatcgc tcttgtagag
    29221 ggcgtagcgc gcgcggtagt cggccaggtt ctgcggcagc ggcaggtcgt gcacgcgcgc
    29281 caggccgctg gtgtctttgg ggctggggtt ctcgtagatg tagtcaccca ggaacagcac
    29341 cagatccggc tcgtcggcgc aggcgtggcg ccaggcggcg tagtaaccgt tctcccagcg
    29401 ctggcatgag gcgtagacca ggcgcagccg cggcgccagt gcatcggcgg ctggggccgt
    29461 gcgcgtgcgg ccggtgcggc tcacggcgtc gtccagcatg aagcggtagt ggtaccagcg
    29521 gcccggctgc aggcccgtca cctccacatg cacgctgtgc gccagctcgg gcagggcggt
    29581 ggcctggccc tgctgcacga tgcgctggaa ctgcgcgtcg tccgcgactt cccagcgcac
    29641 gctgagcggg ccggcccagg gggcgccggc atcggccggg tgctcgggca gcaggcgcgt
    29701 ccagagcacc acgccatcgg gctggggctc gccgctggcc acacccaggc cgaaggggtc
    29761 gtggcgcagc ggctgccggg cccaggccca gcgcggcagc tgcgtggccg tggcagccca
    29821 gaccgcctgt tgcagcagcc ggcggcggct ggtggcctgg gagtgcctgg gcatggagcg
    29881 ttttgggtgc cgctcaggcc gccagcaccc ggttcttgcc ggcgcgcttg gccaggtaca
    29941 tgccctggtc ggcgcggcgt atggcgtcgg cgtggtcgtc gccctccagc tgggtcacgc
    30001 cggcgctgaa ggtgatgagc acgcgctctt tttccttgag aaagtagcgt ttggtcagct
    30061 cgcgctgcag gcgctccatg gccttgacgc cttcggtcag ctcggtgtcg ggcaggatca
    30121 gcacgaactc ctcaccgcca tagcgcgcca gctgatcctg ggggcgcatg acctcgcgtg
    30181 tgaccagcac cagatgcttg agcgcgtcgt ccccggcctc gtggcccagc cggtcattga
    30241 gtgacttgaa gttgtccaga tccagcaggg ccaggcacag cggcttgccc tggcggcgcg
    30301 cgcgggcgat ttcacggcct atggcttcgt caaagccctt gcggttcagc gagccggtga
    30361 gcgggtcgtg gcgcgcctgg atgctggcgc gatcgagctc ctggcgcagc agatccatct
    30421 cctggtgcgt ggtttcggcg cgttcgcgca agtcctgcag ttcggcgcgg gtcaggcggc
    30481 tgttcaaggc caccgcgcgc gtggccgcca tgacctcctc cagcaagggc gtgatctcct
    30541 tgagctccgt ggcctggctg atgcgctccg cacagcgctc catctgttcg ctgtagctgc
    30601 cgctcgattc atccatctgc gccaggcgct cgatgaaggt ggccagcagc tcgcgcatct
    30661 ggctctgtgc ctgcatgctg cgatccttgg cctcggactg cttgaagatc acatccttca
    30721 ggcgctgttg cacgtcgtcg agctggcgca gcgtcaaggg gggagtggag gccgtcagca
    30781 gcgcctcggc ctggccatgc agccaggcgt cgtcgatgct cagggcggag atgttttcga
    30841 acaccaggcg cagcaggccg agcaggctct gacgtatggc ggcctggtct tctgcggcaa
    30901 acgacaggcg atagctgtag ttgcgcagca tctgttcggc cgttgccacg gggggcgtgg
    30961 gttcgcgcaa gaaggcagtg agctgcgcgg caagctcatg gatgcggggg tcgtcttccc
    31021 ccagcgccgg cagggcgttc tccaccatgc gcaccaggtt ctgcgccagg ccatggggca
    31081 ggtggtcgat gctggcgctg tgcacgggcg gcacgggcgc tggcgccagt cccaacttgg
    31141 cgtagcccac gaggacgttc tgcagcatgg accagtcctg ctgcgcaatc gccttctcca
    31201 gcagttcggc ctggcgcttt tgcccaggtg tctggcaggg catgacgcgc aagatctgac
    31261 gcagctgggt ggccggaaac tgcggcgcgg aacgcgttcc tgccacctct tcgtacaggg
    31321 cccggtagtt gtcgggcgat ggagtcagtc ggcgcgtggc cagcagcttc agggtttcac
    31381 gcgcgatgtc ggagggcgat ttggcagagt ccatctgcga acggggggga gacacaaagg
    31441 gccacaattg tgaccgagcg ccgcatattt gaatagctgt ggtgcgcgta gatgtcacct
    31501 cggttaccaa tgccagcttg ccaggcaatg gaacgaacgg ccggtcagac caatggctcg
    31561 cccaccgggt cgtagaacac gtcagggccc tttccagcgc ccacggcctg caccacgccg
    31621 gcgtattccg aaccgggtac aaacggcagg ggcggcttca tctggtgttt gtgctccact
    31681 atcagcagat tggggaagtt gaggctggtg gcacggattt ccatcggcac ctcgcgggcc
    31741 tgggggcggg tgtgggcaat tgagtccatt gcagtgcctc gacgtcgtta cggtggttca
    31801 gggccaggcg cgcatggaag actcatgtcc tttgggtatt ggggcgcatc atggaaggcg
    31861 tggcactgcc ccgtctgtct tgggttcgag cgtgctggac accaggggcc ttctacaatg
    31921 cgttgcattc agtcacattg accatgaaaa ttctcatttc caacgacgat ggctaccagg
    31981 cgccggggat cgtggcgctg catgacgccc tcaaggccat tgccgacgtc gaggtggtgg
    32041 cgccggaaca caacaacagt gccaagtcga acgccttgac gttgcattcg ccgctgtacg
    32101 tgcaccgggc agccaacggt tttcgctatg tgaacggcac gccggccgac tgcgtgcaca
    32161 ttgcgctcac gggcctgctg ggctaccggc cggatctggt cgtctcgggc atcaacaacg
    32221 gcgccaacat gggcgacgac accatttact ccggcaccgt gggtgcagcc atggagggct
    32281 atctgttcgg tattccgtcg attgccttgt cgcaggtgga caagggatgg ggcgagatcg
    32341 attccgcggc gcgcaaggcg tgcgagatcg tggagcagat gcagcggcac catctggtgg
    32401 ggcagtcccc atggctgctc aatatcaaca ttcccaacat gcctttcgag gcgctgcgct
    32461 ccttgcgcct gtgccgcctg ggccggcgcc acgcggccga gcgtgtcata gagcagcaaa
    32521 gtccgcgcgg cgaactgatg tactggatag gcggcgccgg cgccgccaag gatgccgccg
    32581 agggcaccga cttccatgcc acggcgcatg gccatgtgtc catgaccccg ttgaaggtgg
    32641 atctgacaga ccatgatggc ctgggctact gggcccagac cgctgcgcgc ctggctgtgc
    32701 caggcttgtc aggcgggggg ctgtgatgca gcggcgtcct gggtttcccg cgcgcctgga
    32761 caggccgcag gctggggcga tgacgcccac ggttccggct gcgcgcttgg cacgcgagac
    32821 tcagcctccc aagccgcagg gtgtggggct ggattccgcg gcggtgcgcg cgcgcatggt
    32881 gcagcgcctg gccgcggctg gtatcgctgc ggcacctgtg ctgcaggcca tgggggcggt
    32941 ggagcggcat cgcttcgtgg ataccgccct ggcgaaccag gcctatgagg acaccagcct
    33001 gcccatcggc ctgggtcaga ccatctccaa gcccagcgtg gtggcacgca tgatcgaact
    33061 actgctgggc gcggaatgtg cgcgtgccgg cgccctgggc cgggtgctgg aaatcggcac
    33121 cggctgcggc taccaggcgg ccgtgctggc ccggctggcg cgcgaggtgt acaccatcga
    33181 gcgcctgcgc gcgttgcacg aaaaggcgcg tgaacatctg cgcccgctgc gcctggccaa
    33241 tgtgcacctg atcctcggtg acggcatgct gggctatgca gccggcgctc cctatgcggg
    33301 catcatttca gcggcaggcg gcgacagcct gcccatgcag tggtgcgagc aactggccgt
    33361 aggtggacgc ctcgtggcgc cgatggcggg cgccgatgga cgccagctgt tgctggttgt
    33421 ggacaaaacc ctgcagggct tgaaacaaag cgttctcgag gccgtgcatt tcgtcccctt
    33481 aaaatctggg gttgcttgaa ggaattgctt atgttggtat cgcgtggtct tgtggcttgg
    33541 gggatggtgg ctctggctgg catggggctg gtgggttgcg gcactccggt gaacaaggct
    33601 ccggtcgtgg atcgtggcac ctttgccaat ggcggcgcta cgccgcctcc tgccgcgccc
    33661 gaggtcaaac ccctgccggg ggctgagaac gccggcaagc cgggttatta caccgtcaag
    33721 ccaggggaca ccctgattcg cattggcctg gataacgggc agagctggaa agacatcgca
    33781 cgttggaaca atctggataa cgccaacctg atcgaggtcg gccaggtgtt gcgcgtggtt
    33841 ccgccagggg cgccgagttc tgcggtggca tccgacacgg gtgtcgtcac gcggccggtc
    33901 acgacgtcgc ccgtggcacc atcctcgtcg gccagtgcgc ccaaggcggg gcctccgacc
    33961 gcagcggctt ccactccagc ggcgccggtg gagccgattg ctgcgcccgc gccggcacca
    34021 gcgccggcac ccgcgccggc gcccgccgtg gctggtggtg tggacgacat gcatttcatc
    34081 tggccctcgt caggctccct gctggcgggc tttgacgagg cgcgcaacaa gggtttcgat
    34141 attgctggca aggcgggtga tccggtactg gccgcggccg atggccgcgt ggtgtatgcc
    34201 ggcgccggcc tgcgtggcta tggcaacctg gtgatcctca agcacaacaa cacctttctc
    34261 acggcctacg cgcacaacca gacgctgctg gtgaaggaag accagaacgt gcgccggggc
    34321 cagaagatcg ccgagatggg cagcaccgat gccgaccggg tgaagctgca cttcgaaatt
    34381 cgccgccagg gcaagccggt cgatccggcc cgctacctgc cagcgcgttg agcacgccgg
    34441 tggccggcga cggctttcag tggccggacg aggagtccga gccgcaggat tcaggcgaag
    34501 cctgcgcatc ggggcaggcc gatgcggatc tggagctgcg ccaagtggag gtgtctgcag
    34561 tgcagcacca ggttcggctg gacaaggccc tggcagagct ggttccagag ttttcccgca
    34621 gctatctgca gcagttgctg tcccaggggt gcgtacgcct taatgaccag gtggcgcata
    34681 aacccgcgca caaggtcagg gccggcgatc gcgtggtggt ggagatgcgc ccaacgcagc
    34741 aaagccaggc cttcaaaccc gagcaattac ccctggacgt ggtgtacgag gatgcccatt
    34801 tgctggtcgt caacaaacca gccgggctgg tggtgcatcc ggcgccgggc aactggagtg
    34861 gcaccttgct caatgccttg ctcgggcgcg atgcgcaggc gctgcaagtg ccgcgcgccg
    34921 gcatagtgca ccggttggac aaggacacca gcggcctcat ggtggtggcg cgcagccgcc
    34981 cggtcatgga tgcgctggtg cgcatgatcg cggcgcgcga ggtcagtcgc cagtatctgg
    35041 cgctggcctg ggggcggtgg gcgggtgcgg cgcagcgcgt tgttcaggcg cccattggtc
    35101 gagacccgcg caatcgcctg cgcatggcga cggtcgatct gcagcagcac ccgggcaagc
    35161 cggcgcgcac cgacttcgag ctgctggtcg gcaacgatga ggtgtgcctg gtgcgctgca
    35221 cattgcacac cgggcgcacg caccagatcc gcgtgcacat ggcgtccctg cgccaccccc
    35281 tggtggccga cgggctgtac ggcggcgcac tcgggggcgt cctgcagcgc caggccttgc
    35341 atgcgtttcg cctggcgttc cagcatcccg tcaccggaca ggccatggag ttgcgcgcgc
    35401 cgctgccagc ggacatgctg caggcccttg atttctgggc tctgggctac aatggcggct
    35461 gacggctgat cagccgctgg tgctgccggt tgcatactgc gtaccgcatg ttggccccat
    35521 gatggcgcgc tgccacttgc agcactttcg cgccgcagat gtcctccaat cccaggcaac
    35581 cactccgctg cggagtacgg aagccctcat cggaactgct gaagcatgaa ttcggtggat
    35641 gccaaacggg ttcttgaaac cgcgttgatc tgcgccccgc agcccgtgac cgtgcgcgaa
    35701 ttgcgtaccc tgttcgacga tgcgctgggc tcggatacgg tgaagatgct gttgctgcag
    35761 ttgcagcagg attgggcctt gcgcggggtg gagttggtac aggtggcgac ggggtggcgt
    35821 tttcaaagcc gccccgaaat gcgcgagtac ctggatcgac tgcacccgga aaaacccccg
    35881 cgctataccc gggccacgct ggagaccctg gccatcatcg cctaccgcca gccggtgacg
    35941 cgcggcgata tcgaggacat acgcggcgtg accgtgaaca gcctgatcat caagcagctg
    36001 gaggacaggg gctgggtcga ggtcataggc catcgcgaga cagttgggcg cccggcattg
    36061 ttcgccacta cgcggcagtt tctggacgat ctgggtctgc agtcgctgga tcagttgccc
    36121 acgctggacg accccggcgt tcaggccacg gtatttgaag cgctggccga tgcccccgag
    36181 gtgcagcggc cagacaagtt ggagatgaaa gtggccctgg atattgctga aacgccagct
    36241 cagggcgatg cggacatggc agacgccccc gtcgagtccg gtggtgtgga aaacgaggaa
    36301 gcagacgatg gcgtcacagg aacatgatat gaatgacacg cacaaggatc tggatgccgc
    36361 gcagccggct gcccaggcgc cggccggctc cgttgacgag cccagggcgc gccggccgcg
    36421 ccagcgcaaa ccgggccaga tcgcagattc agcctcattc gcgcagcagc ccatggcgca
    36481 gcaggccgaa gaaccaggcc agaaaccgca acgcgactcc cgtggcccgc agggcaggcg
    36541 gggcggcaag tcttctgccg atcgcgaagg ctatcggttt gccgacgtgg tctcgggcca
    36601 gctcgaccag gatgagactg acaccgaggt ggcgcccgcc aaacgcgtgc tgctgcccca
    36661 gccggagacg cccaagctgc acaaggtgct ggcccaggcc ggcatgggtt cgcgcctgga
    36721 gatggaacag ctcattcttg aagggcgtat ctcggtgaac aacgagcccg cccatgtcgg
    36781 ccagcgcgtg cagtttggcg atcagatcaa ggtcaatggc aaacccatcc gttttcgtat
    36841 tgcgccgcca ccgacgcgcg tgattgccta tcacaagccg gccggggaaa tcgtgacgca
    36901 cgatgacccg cagaaccgcc ccacggtgtt ccgcaagctg ccgcggctgg tgcagggcaa
    36961 atggcagtcg gttgggcggc tggatttgaa caccgaaggc ctgctgctgt tcaccagctc
    37021 gggggaactg gccaacaggc tcatgcaccc gcgctttggc ctggagcggg agtacgcggt
    37081 gcgcgtgctg ggcgctttga gcgacgagga aaagcagcgc ctgctcgatg gtgtgcagct
    37141 cgaggacggc atggcggcct ttggctccat tgacgagggc gggggcgagg gcgccaattg
    37201 ctggtaccgc gtgacgattt ccgaggggcg caaccgcgag gtcaggcgca tgttggaggc
    37261 cgtggggcat gcggtcagcc ggttgatccg catacgctac ggcgccatgc tgctgccgcg
    37321 cggcttgaag cgcggcgcct ggatggagtt ggacgagcac gatattcagg ccctgatgca
    37381 ggccgccggt gccccgctgg atggcgcagc tgcaggccgc cagccgggcg ggggcgggca
    37441 gggcaacaac gcacaccgtg gcgagcgcaa gcccatgggc ggcaagatgg ctggtggcgg
    37501 tcgcagcaag agcctggcgc gcgatgggcg atctgccgga aatacccagc ccgacccgat
    37561 gaaaacctcg gtcggctata tcggcgccga cagtttcctg cgccagcgtc agcagcaacg
    37621 cagcggtggc ggcgcgcgcc gcggccgccg atagggaatt ggcgaaaagt tagaatgtgc
    37681 ggttttgcct acatggcaaa aaaaccgcaa caccctactc aaggaaatca tatggctatc
    37741 gaacgcaccc tctccatcat caagcccgac gccgtcgcca agaatgtgat cggccagatc
    37801 tacgcccgct tcgaggccgc cggcctgaag atcgcggctg cccgcatgat ccacctgtcg
    37861 cgcgccgagg ccgagcagtt ctacagtgtg cacaaggagc gccccttctt caaggatctg
    37921 gtggacttca tgatttccgg cccggtgatg gtgcaggtgc tcgaaggcga gaacgccgtg
    37981 ctgaagaacc gtgagctgat gggcgccacc gaccccaaga aggctgatgc cggcaccatc
    38041 cgcgccgatt ttgccgacag catcgacgcc aacgccgtgc acggctccga tgccgccgag
    38101 accgctgccg tcgaagtggc cttcttcttc cctggcctga acgtttacgc acgctgatgc
    38161 ggcggccgcg ggtgccgtgc aggcctgacc aggatctgca ggcgcccggg gcgcactatg
    38221 tgggcgtgga catccgatat acgttatgac cacgaacctt ctggactttg acctggatgc
    38281 catggccgcc ttttgcgagc ggctgggcga gaagcgcttc cgggccacgc aactgtttcg
    38341 ctggatccac cagcgcggtg cgcgcgattt cgaccagatg agcgatctgg cgaaatccct
    38401 gcgcgaaaaa ctcaagggct gcgcccatgt gacgggcttg caggccatca gtgaacatgt
    38461 gtcgtcggac ggcacggtga agtggctgtt cgacgtgggc ggcggcaatg ccgtggagac
    38521 ggtgttcatc cccgaagacg accgcggcac gctgtgcatc tcgtcgcagg cgggctgtgc
    38581 cgtgggctgc cgcttctgct cgacggggca tcagggcttc agccgcaacc tggcgtcggg
    38641 cgaaatcatt gcccagctct ggcatgccga gcatgtgctg cgccagcgcc gcggcgatgg
    38701 cgagcgcgtg atctccaacg tggtgatgat gggcatgggc gagccgctgc agaactactc
    38761 ggccctggtg ccggcgttgc gcatcatgct ggacgatcat ggctacggtc tgtcgcgccg
    38821 ccgcgtcacg gtgtccacct cgggcgtggt gccgatgatg gatcgcctgg gcgcggactg
    38881 cccagtggcc ttggccgtgt cgctgcatgc gcccaacgac gcgctgcgcg acaacctggt
    38941 gccgctcaat cgcaaatacc cgctgcacga gttgctggct gcctgcaggc gctacctcga
    39001 tcacgcgccg cgtgacttca tcacctttga atactgcatg ctcgatggcg tgaacgacca
    39061 gccagagcat gcgcgcgagc tgattgaact ggtgggccgc agggcggccg atggcggcgt
    39121 accctgcaag ttcaacctga ttcccttcaa cccgtttccc gcatcgggcc tgcaccgctc
    39181 gcccgcggcg gcggtgtcgg ctttcgccaa gctgctcagc gatgccggca tcgtgaccac
    39241 tgtgcgcaag acgcgcggcg acgatatcga tgcggcctgc ggccagctgg cgggtgatgt
    39301 gaaggatcgc acccgcgtgc atgaacgcat ggccaagctg cgggtcatag aaatcaagcc
    39361 tgtccattga ggcttgtcgt aaccgagaga ggcgatacat gaggctgtgg ggcgggggag
    39421 gaattcggca cgggctgctg gcggcctgtt tggcggtggg ggccgccgcg ctgggtggat
    39481 gcgcgcatca ggcggggctt gactccggtg tcggcgccgc cgagccggtg tctgcttccg
    39541 gcgataccga tgtgcgccgg cgtgcccgca tccggctgga gctggccgcc aactatcttc
    39601 agatggggca gaccggtgtc gctctggagg aggtgcagca ggcgcttgcc accgacccca
    39661 gctatgccga tgcctaccat ctgcgcggac tggtctacat ggccctggct gacctgcagg
    39721 gtgccgaaga cagcctcaag cgcgcccagg ccatgcggcc caatgatccg gacatcatgc
    39781 acaactacgg ctggctgcaa tgccagcgcc agcagtacgc acaggcgaat cagctgtttg
    39841 agcgcgccct ggccagtccc acctatgcct cgcgcagcaa gacgctgatg tcccagggcc
    39901 tgtgctacca aagagcaggg caggtggccg aggccgagaa gacattgctg cgcgcctatg
    39961 agatagatgc cggcaatccg ttggtcggct accagctggc gtcgatattg ctggcacgcg
    40021 aggagcccaa gcgggcgcag ttttatatcc gccgagtgaa caacggcgag ttctccaatg
    40081 ccgcgtcgct gtggctgggt gtgaaggtgg agcgcgcgct tggggattcc gtggccatgc
    40141 gccaattggg cgagcagctg cacaagcgct tccccgatgc caaggaaact ttggcgtttg
    40201 agagaggggc ttttcatgag tgagtcgatc ggagagcaca acgccgatca atccgtgcca
    40261 gcaccggcac cgaacgcagg cggcatgtcc gccggcacat ggctgcggca ggtgcgcgag
    40321 tcggccggct tgcacatcga tgtgctggcc ggcgccctga aggtgccggt ggccaagctc
    40381 caggcgctgg aagccgatga ctatgcggcc tttcccgatg ccgttttcat gcgtgcgctg
    40441 gcatcgagca tgtgccgcgc gctgaaggtg gacgctgctc cggtgctggc gctcctgccc
    40501 cagggcgcgc ccatccatct gccgccggac agggccatca acgcttcatt caaggattct
    40561 ggtcgccgcc tgggcaaggg cggctccatc gagcggccca agtcacgcgc catgggcctg
    40621 gctgtcgtgg ccttgctgct gggtgcgttg gcggtggcct ttctgccgct gggcggcgat
    40681 ggcaagcaag accagcccgc gctggccatg acgcctgcac cgcaggcgcc ggccgcaccg
    40741 gtggtggcgg cgcaagagcc cgaggcggtg gtgccagctg ccgagccgac aggcgccgcg
    40801 cctgaggcgc agccggccga cggcacgccc gtggcacagg agccggccgc tgccgtggct
    40861 accgctgctg tgcaaggcgc tctggtcatc cgcgcgcaca aggaatcctg ggtgcaggta
    40921 cgcgatgccg gtgggcgcgt gttgttgcaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41041 nnnnnnnnnn ncacaaggaa tcctgggtgc aggtacgcga tgccggtggg cgcgtgttgt
    41101 tgcaaaaggc attggctgcg ggcgagagct actcggccga gggcagtccg ccgtggcggg
    41161 tggtgattgg cagggccgac gccaccgagg tcatcgtgcg tgaccagccc atggatctga
    41221 aggccgtctc gcgtgagaac gtggcccgtt tcgaggtgaa gtaaatgaat ccaaccgacg
    41281 cacccattgc catggctgcg cccctggcgc gccgttcgcg ccaggcaaga attgtctggg
    41341 gcagcaacgt ggtcaccgtg ggcggcgacg ctcccgtgcg tgtgcagtcc atgaccaaca
    41401 ccgacacggt ggacgcgatt gccacggcca tccaggtcaa ggagctggcg caggccggct
    41461 ccgagttcgt gcgcatcacg gtgaacacgc ccgaggcggc ggccgccgtg ccctatattc
    41521 gcgagcagct cgaccgcatg ggctgcgatg tgccgctggt gggtgacttc cactacaacg
    41581 gccaccgcct gctgaccgag tttccggatt gcgcccaggc gctgtccaag taccgcatca
    41641 atcccggcaa cgtgggcaag ggcgacaagc gcgacaggca gttcggccag atgatcgagg
    41701 cggccgtgcg ctgggacaag gccgtgcgca taggcgtgaa ctggggcagc ctggatcagg
    41761 agctgctggc cgggctgatg gacgtcaacg gccgccgtag ccagccctgg gacgcgcgcc
    41821 aggtcatgta cgaggcgctg atcacctcgg ccatcgaatc ggcgcgtcgc gccgaagaaa
    41881 tgggcctgcg cggcgagcag atcatcctgt catgcaaggt cagcggcgtg caggatctga
    41941 tcgccgtgta tcgtgaactg gcgcgccgct gcgactacgc cctgcacctg ggcctgaccg
    42001 aggccggcat gggtaccaag ggggtcgtgg cctcggccac ggccctgtcc atcctgctgc
    42061 aggagggcat cggcgacacg attcgcgtgt cgctcacgcc ccagccgggc gaggcgcgta
    42121 cgcaagaggt ggtggtcgcc gccgagatat tgcagtcgct gggtctgcgc gccttcgtgc
    42181 ccagcgtgac cgcctgcccc ggctgcgggc gcaccaccag caccaccttc caggagctgg
    42241 ccaagcagat cgacgactac ctgcgcgcgc agatgcccgt gtggcgcgag cgctatccgg
    42301 gtgtggaaaa gctcaaggtc gcggtcatgg gctgcatcgt caacggcccg ggcgagagca
    42361 agcatgccga catcggcatc agcctgcctg gaaccggcga ggcacccgcg gccccggtgt
    42421 tcatcgatgg cgagaaagcc atgaccctgc gcggcgagaa catcgtgcgc gagttccagg
    42481 acatcgtcga tggctacatc gacaagcgct ttggattggg tgcgtaggta tcaaaataat
    42541 agctgtcagc gcttgcctat aaagcgttag agcccgattt cattcaaata atagattcac
    42601 acaagatgca gaagctggtt gccatcaagg gcatgaacga catcctgccg cccgattcgg
    42661 cgcgctggga atggctggag gagaaggtgc gctcgctgat ggcgcgctac gcctaccgca
    42721 acatccgcac ccccatcgtc gagcccacgc cgctgttcgt gcgcggcctg ggtgaggtca
    42781 ctgacatcgt cgagaaggag atgtattcct tcgaggaccg cctgaacggc gagcagctca
    42841 cgctgcgccc cgaggccacc gccggcgtgg tgcgcgccgt ggtcgagcac aacatgctgt
    42901 acgacggcgg caagcgcctg tactacatgg gcccgatgtt ccgccacgag cggccacagc
    42961 gcgggcgcta ccgccagttc caccagatag gcgccgaggc cctgggcttt ggcggccccg
    43021 aggtggacgc cgagatcatc ctgctggcgc acgccctgtg ggccgagttg ggtttgacca
    43081 atgtccgcct ggagctcaac agcctgggcc agcccgacga gcgccgcgcc caccgcgccg
    43141 cgctgattgc ctacctggag cagcaccagg acgtgatgga cgaggaggcc aggcgccgca
    43201 tgtacagcaa cccgctgcgc gtgctcgata ccaagaaccc ggccatgcag gacatggtga
    43261 acgccgcgcc gcggctcatc gactttctgg gcgaggcctc gctcaggcac ttcgacaccg
    43321 tcaaggccat cctggacgcc aacggcgtcg cctggagcat caacccgcgc ctggtgcgcg
    43381 gcatggacta ctacaacctc acggtgttcg agttcatcac cgagcagttg ggctcgcagg
    43441 gcacgatctg cggcggcggc cgctacgact acctcatcga gcagatcggt ggcaagagcg
    43501 ccccggccgt cggctgggcc ttgggcgtgg agcgcgtgct ggagctgctc aaggagcagg
    43561 cggcccaggc gccgcagccc gccgctgacg cctacgccgt ggtgcccgat gcggcggccc
    43621 tgccccaggt catggtctgc ctgcaacaat tgcgcgccct gggcgtggcg gtgcaaatgc
    43681 acagcccggc caactccgcc gaaggcatgg gcagcatgaa gtcgcaattc aagaaagccg
    43741 acgccagtgg cgcgcgcttt gctctgattt ttggtagtga tgagctggca cgcggcgtcg
    43801 taacagtcaa acccctgcgc gacggcggcg agcaggtcga gcggcccctc gccaccccgg
    43861 ccgactgggc tgccagccta caatcgccgc gctgaacgga aaccccaaca tggcaaacaa
    43921 ctttgacctg caagaacaag aacagctcga ggagctgaag catttctgga acaaatgggg
    43981 cacgcccatc acctgggcgc tggtcgtcat catgggcagc tttgccgcat ggaacggcta
    44041 ccgactctgg caaggccgcc aggcccagca ggccaccgca ctggtggagg ccgtggaact
    44101 ggctgcgcag acgggcgaca tggcgcgcgt ggagcaggcc tttggtgacc tgcagtccgg
    44161 ctatggcggc accacccagg ccggccaggc aggcctgctg gcagccaagg ccctggccga
    44221 cgccggcaaa tgggatgcgg ccaagggcgt gttgacctgg gtggccgaga aatcctccga
    44281 tgagggctac gtggcactgg ccaggctgcg gctggccggg gtgctgatgg agcagaaggc
    44341 ctatgacgca gcgatggcgc aactgtcggg cagcatgccg gcagagtttg ccggggtcgt
    44401 ggcagatcgc aagggcgacg tgttggcact gctgggcaaa aatcaggagg cgatcgccga
    44461 gtaccgccgt gcctaccagg cctttggtga cagcgtggaa taccgtcgcc tggtcgaagc
    44521 caagctgaac gctctgggcg cgcaggcgca gaacgtggcc gttgccgcac cggccgggag
    44581 cgcgcagtga tcgccgcgcg cagaaacttc cgcgtcttga cgctgacgct ctcgctggcc
    44641 gcgctgctgg gcgcgagcgg ttgttcgctg tggggcgggt cgcccaagcc caagccggct
    44701 gaattggggc cgaatgttcc cgtgctcggc gtacgccagg cctggacggc caagatcggt
    44761 gccatggacg gcctggcgct ggatgtccat gtgaacgcca acacgctcac cctggctgcc
    44821 gccaatggtg aagtggcggc catcgacgca cgcaccggtg gcgatgtctg gcgcacccag
    44881 ctgaacctgc cgttggccgc gggcgtgggc agcgatggcc gctgggctgc cgtggtttcc
    44941 cagggcgcgc agctcatcgt gctcgatggc gggcgtgaaa tctggcgcaa gccactgacg
    45001 gcgcaggcct atacggcgcc gctggtggcg ggcaatcggg tgttcgtgct ggcggccgat
    45061 cgctccgttt cggcttatga cgcggccacg ggctaccgcc tgtggggcca gcagcgcccg
    45121 ggcgagccct tgatactgcg ccaggctggc gtgctgatgg ccgtgggcga caccctggtg
    45181 gcgggccttt cggggcgcct ggtgggcttc aaccctgaca acggggtggt gcgctgggag
    45241 gcgcccctgg ccagcccgcg cggtaccaat gatgtggagc gcctggttga aatcgtcggc
    45301 cacaccagcc gcgtgggtga caacctgtgt gcgcgcgcct tccaggccac agtgggctgc
    45361 atagacgcgg cccgcggtgc ggtgctttgg acgcagccgg ccggcggcag cgagggcctg
    45421 gacggcgacg ccgagatgct gtttggcacc gagagcaatg gcacggtcgt cgcctggcag
    45481 cgcgccgatg gcaagcgtgc ctggacgtcc gagcggctac agtttcgcaa gctgacggcg
    45541 ccgctgctgc tgggccgttc ggtcgtcatt ggcgacgacg cgggcctggt gcatctgctg
    45601 tcgcgtgcgg acgcggcgcc gctgaaccgc ctggccacgg atggctcggg cattgccgcg
    45661 gcgcccgtgg ccgccggcga cacgctggtg gtggtcacgc gcaagggcgg cgtctatggc
    45721 tttcgccccg actgattcag ataggacact gggatgaaac cggtcattgc cctggtgggg
    45781 cggcccaatg tgggcaaatc caccctgttc aaccgcatga ccaagtcgcg cgacgccatc
    45841 gtggccgact ttgccggcct cacgcgcgac cgccactacg gcaatggccg ccagggccag
    45901 cacgaataca tcgtcatcga cacggggggc ttcgagcccg atgccggtag cggcatcttc
    45961 cgcgagatgg ccaagcagac gcgccaggcc gtggcagagg ccgatgtggt ggtcttcgtg
    46021 gtggacgtgc gcggcggcct gtcggcgcag gatcatgaca ttgccaacta cctgcgccgc
    46081 ctgggcaagc cgtgcgtgct cgccggcaac aaagccgagg gcatgcagga cagcccccat
    46141 ctggccgagt tctatgaact gggcctgggc gaggtgcatc cggtgtcggc cgcacatggt
    46201 cagggcgtgc gcagtctggt cgagctggcg ctcgaacccc tggctctgcc agaggtggat
    46261 gagtgcgaag ccggcgccga ggccaacgtc atacgcctgg ccgtcgccgg gcgccccaat
    46321 gtcggcaaat ccacgctgat caacacctgg ctgggcgagg agcgcctggt ggccttcgat
    46381 ctgccgggca cgacgcgcga cgccatctcg gtgccgttcg agcgcaatgg ccagcggttc
    46441 gagctgatcg atactgcagg cctgcgccgc aagggcaagg tgttcgaggc catcgagaag
    46501 ttctccgtcg tcaaaaccct gcaggccatc gaatcggcca acgtggtgct gctgttgctc
    46561 gatgccactc agggcgtgac ggatcaggac gcgcatatcg cgggctatat cctggaaagt
    46621 ggccgcgccg tggtgattgc cgtgaacaag tgggacgcgg tggacgacta tcagcgccag
    46681 cagctggagc gctccattga gtcgcgcctg tccttcctca agtttgcccc gctgcatttc
    46741 atctccgcca tcaagcgcca gggcatagcg ccgctgtgga cttccatcgc gcaggcctac
    46801 aagtcggcca accgcaagat gcccacgccg gtgctcacgc gcctgctgca cgaagcagtg
    46861 caattccaga ccccgaagcg tgccggcggc ttccgtccca agctgcgcta tgcgcaccag
    46921 ggtggcatga atccaccggt gatcgtgatc cacggcaatt ccctggaaca tgtctcggat
    46981 gcctacaagc gctttctgga ggggcgtttc cgcaaggagt tcgacctggt tggcacaccg
    47041 ctgcgcatag aaatgaaaac ctccagcaat ccttacaccg acaagaacgg cgcctgagcc
    47101 cttgcgctgc atatggcagc gcggctgtgg taaggtgccg ctttacaaca acatttttga
    47161 acacggagaa tatcgtgagc aataaaggtc agcttcttca agaccccttc ctgaacgcac
    47221 tgcgtcgcga gcatgtgccg gtgtccatct acctggtcaa cggtatcaag ctgcaggggc
    47281 agatcgagtc cttcgatcaa tatgtcgtgt tgttgcgcaa cacggtcacg cagatggtct
    47341 acaagcacgc tatctccacc atcgtgccgg ggcgtgccgt caatttctct acggtagaaa
    47401 ccgccgaggg cagcaatcag taagccagca acgcagcttg aggcattgcc agggtccaag
    47461 cgcttcgcat gcaaggatcc gcagccttga gttccaagcc atcttccgat gcccgcacca
    47521 cgccggtcct gttgatcggc gtggattttg gtgcgccgca tttcgacgcc gacttgcagg
    47581 agctgggctt gctggcacag accgcgggcc tgaatccggt agctcgactg acgtgcaagc
    47641 gcaaggcgcc ggatgcagcg ctcttcgtcg gcagcggcaa ggccgacgag attcgaaccc
    47701 tggcccagat gcatggtgcc caggaagtgt tgttcgacca ggccctgagt ccggcacagc
    47761 agcgcaacct ggagcgccat ctgcagctgc cggtgaacga ccgcaccctg ctgatcctgg
    47821 agattttcgc ccagcgtgcg cgtagccacg agggcaagct gcaggtggag ttggcgcgtc
    47881 tgcagtacct cagcacccgg ctggtgcgcc gctggtccca cctggagcgc cagcagggcg
    47941 gcatcggcat gcgcggcggc ccgggcgaga cgcagatcga gctggacaag cgcatgatcg
    48001 gcgacgccat caagcgcact cgcgagcgtt tgctcaaggt caagcgccag cgcgcgaccc
    48061 agcggcgcca gcgcgagcgc cgtgatacct acaacatctc cctggttggc tacaccaacg
    48121 cgggcaagtc caccttattc aatgcgttgg tgaaggcgcg cgcatacgcc gccgaccagc
    48181 tgtttgccac gctggacacc acgacgcggc agctctacct gggcgaggcg gccggctcgg
    48241 catccctgtc ggatacggtg ggtttcatcc gtgatctgcc ccacgggttg gtcgatgcct
    48301 ttcaggccac attgcaggag gcggtggatg ccgatctgct gttgcatgtg gtggatgcgg
    48361 ccaaccctga ctacccggag cagattgccc aggtgcaaat ggtgttggcg gaaattggcg
    48421 cctcggacat tccgcagtta ctggtattca acaaactcga tgccatggcg ctcgaatcca
    48481 ggcccaagca attgcaggac atgtacgcac tggatgggcg caaggtgccg cgcatcttcg
    48541 tgagcgcacg ccagggcgaa ggggtggcgg cattgcgtga acagctggcg caggtggtgg
    48601 catccgcggc cagtcccaac agcatgtccc ctgatcaggc cactgaattg ccgaatcccc
    48661 atgtttgatt gggcacaatg ccggttgtta cagatctaca aagaaccaga gaacttgcgc
    48721 atgaatcttc ctaaccgtgc ctcgcgctgg gcattgttgc ccgagcgcat ccgagggatg
    48781 ttcaatctca acgatccgcg ctggggccgt ggtgacgaca agggcgaatc agatgagcgc
    48841 ccccagcagc cggaacgccc caacaacgac aggccgcagg gcggcagagg gcgtgaccag
    48901 ggtccaggtg ggcaaccgcc ggacctcgat gaactctggc gtgacctcaa tcgcaagctg
    48961 agcggcttat ttggaggtgg caatggcggt ggccgagggc gcatgccagg cggcggcaat
    49021 ggcggcggtg gcttgcaacc cgacatgaag aacgccgggg taggcgttgg tctgatcgcg
    49081 gccatagccg ttttgatctg gctggggacg ggctttttca tcgtgcagga aggccagcag
    49141 gccgtgatca cgcagttcgg caagtacaag agcacggtaa atgccggctt caactggcgc
    49201 ttgccttatc ccatccagcg tcacgagctg gtgttcgtga cccagatccg ttcggctgac
    49261 gttgggcgcg acaccatcat caagagcacg ggtctacgcg aatcggcgat gctgacagaa
    49321 gacgagaaca tcgtcgaaat caagtttgcc gttcaatacc gcttgaatga tgccagggca
    49381 tggctgttcg agagccgtaa cccggctgat gccgtggtgc aggtggcgga gacggcggtg
    49441 cgtgaagtcg ttggcaagat gaagatggac actgcgctgg ctgaagaacg cgaccagatt
    49501 gccccgcgcg tgcgcaacct gatgcaaagc attctggatc gctacaagat tggcgtggaa
    49561 gtggtgggca tcaacctgca gcagggcggc gtgcgtccgc cggagcaggt gcaggcggcg
    49621 tttgatgacg tgctcaaggc gggtcaggag cgcgagcgcg caaagaacga ggcgcaggcc
    49681 tatgccaatg acgtgattcc gcgtgcagtg ggtacggcgt cgcgcctggg agaagaagcc
    49741 gccgcttaca aggcgcgcat cgtggcccag tcccagggtg atgcgcagcg tttcgctgcc
    49801 gtgctgacgg agtaccagaa agccccccag gtaacacgcg accgcatgta tatcgaatcc
    49861 atgcagcaga tctacagcaa tgtcaccaag gtgctggtgg agtcgcgcca gggttccaat
    49921 ctgctgtatc tgcctttgga caagatcatg cagggtgtct cgcaaacact gccagcggca
    49981 actccagagg ctccgcctgc cggatccact ggcactgctg caccctctca atttcccagc
    50041 gactcccgta tcacgcgcga ccccaaccgc tcgcgcgaac gtgagtctcg ataggagatc
    50101 tgtgtgaaca gaatcggatt ttttgtttcc agcatcctgg tgctgctggg tttggccagc
    50161 tccatgatgt tcgtggtcga tcagcgtcag tttggtgtgg tgtatgcact gggacagatc
    50221 aaggaagtga tcaccgagcc tggcctgaac ttcaagcttc cgcctccatt ccagaacgtg
    50281 cgctatatcg acaagcgctt gttgacgctg gacagctcgg acaccgagtc catgctgacg
    50341 gcggagaagc agcgcgtggt gatcgactgg tatgtgcgtt ggcgtatttc cgatccgcag
    50401 gaatacatcc gcaatgtcgg cctggatgag agtgcgggcg ccatgcagct caatcgtgtg
    50461 gtgcgcaatg ccttccagga agaggtcaat cgtcgcaccg tgagggagct gctatcgagc
    50521 aagcgtgatg ccctgatgga agacgtcaag cgcgaggtgc tggaggccgt gcgtggcgcc
    50581 aagccatggg gcgtggatgt ggtggacgtg cgtatcacgc gcgtggacta tgtggaagcg
    50641 atcaccgagt ccgtgtaccg acgcatggag gccgagcgta agcgtgtggc caacgagctg
    50701 cgctcgaccg gcgccgccga gggcgaaaaa atccgcgccg acgccgatcg ccagcgggag
    50761 atcgccatcg cgaacgccta ccgtgatgcg cagaagatca agggtgaggg cgacgctgaa
    50821 gcggcgcgcc agtacgcaga ggcctttggg cgtgatccgc agtttgctca gttctatcgc
    50881 agcctggagg cctacaaggc cagcttcaac cgcaagagcg acgtggtggt gcttgatcca
    50941 tcctccaccg atttcttcaa ggcgtttcgt ggcggcgcgg cacccggcac gttagcggct
    51001 cccggcgctg taccccgcaa gccctgagca gactttcatt gcggctcatg gactgggcag
    51061 acagtttttg gatggctctc gggctggtgt tggtgatcga agggattttt cccttcgttt
    51121 cgcccactgg ctggcggcaa atgttcacgc agattctgca attgcgtgac gggcagatcc
    51181 ggttttgtgc gctgctcagc atgcttggcg gcgctctggt tctcctgctg ttgtcatagt
    51241 ggcagcgggt acgggtgctt actgcgcgtg ccggtagaat cgttctttta acagccttcc
    51301 ccactccatg tctgcctggg tcctgccgga tcacattgcc gatgtattgc cctccgaggc
    51361 gcggcacatc gaagaacttc gtcgcgggtt gctcgatacc gctcgcagct acggctacga
    51421 gttggttatg ccgccgctgc ttgagcatct ggactcgctt ttgaccggca ccggcgaggc
    51481 gctggatctg cagaccttca aattggtgga tcagctctca ggtcgctcgc tggggctgcg
    51541 cgcagacact acgcagcaag tggcccgcat cgacgcccat ttgctcaatc gcaagggcgt
    51601 gacgaggctg tgctactgcg gccccgtgct gcacacgcgt ccggatcggc cccatgccac
    51661 acgcgagccc ctgcagtttg gtgcggagat atacggtcac ccaggcatgg aggcagatat
    51721 cgaggccgtc cagctctcgc tggaatgctt gcgcggcgca catgtgcagg acgtgagcgt
    51781 cgatctggcc gacgtgcgta tcgtgcgcag cctgctggcg ggggttccgg tgggctggca
    51841 gaccctgggt cgcgtacatg gggcgctggc ggcaaaagac atcagcgagc tgacatcgct
    51901 gacccgcgat tttcctgctg cttcgcgcca ggggctcttg gcgctgttgc agctgtatgg
    51961 ggatattggc gtcttggatg aggcggaaat cgccctgaaa tccatagctg gcgtgcgcaa
    52021 cgtgctatcg gatttgaggg cattggcgtc cagcatccaa ggtgcacgag tgacctttga
    52081 cctggctgat ctgcgcggct atgcgtacta cagtggcgcc cgttttgcca tctacaccca
    52141 gggcgcaagc gatgcgctgg tgcgtggtgg tcgctatgac gaggtgggtg cggtattcgg
    52201 tcgcaaccgc cctgcagcgg gtttcagcct ggatatcaag caactggtca gcgtcgtttc
    52261 gccccgtccg ctcaaggcag ccatccgcgc gccttggggg gaagggcagg gggcaaatgc
    52321 agcgatcgca tccttgcgct cgcaaggcga gactgtggtg tgcatgctgc cggggcatgg
    52381 cagcgaggtg gatgaattcc gctgcgaccg tgagctgatc cgggttggtg atcgctgggt
    52441 tgtccaggcc atctgatttt tgatttttcc agtatttgaa tcgggtttga catgaaaact
    52501 agcaaaggac gcaacgtcgt cgtggtcggt acccagtggg gtgacgaggg caagggcaag
    52561 ctcgtcgact ggctcaccga aagcgcacag ggggtggtgc gcttccaagg cggccacaac
    52621 gctggccata cattggtcat caacggggtc aagacggcgt tgcacctgat tcccagcggc
    52681 atcatgcgcc caggcgtgaa gtgctacatc ggcaatggcg tagtgctttc cgcagccaag
    52741 ctgttcgagg aaatcgaggg gctggaaaag gctggcgtgg aggtgcggtc gcgccttcgt
    52801 gtcagcgagg cttgcccgtt gatcctgccc ttccatgtgg ccctcgacgt ggcccgcgag
    52861 gcagcgcgtg agcatggtgg cgtcgaaaaa atcggtacca cggggcgtgg cattggcccg
    52921 tcctacgagg acaagatcgc gcggcgtgcg ctgcgcgtgc aagatctgaa gtatccggag
    52981 cggtttgtcg cgaagttgcg cgagttgctg gccttgcaca accatgtgtt gagcaccttc
    53041 ctgggttccg gcagttttca gttcagcgat gcgctcaggc catacatgaa agacgggcag
    53101 gttcagttcg atccggtttt cgccgaggcc atgcgccatg ccgagatgct caggcccatg
    53161 atggcggacg tgtcgcgcga gctgaacgaa gcccatgcag cgggtgccaa cctgctgttc
    53221 gaaggtgcac agggcacact gctggatgtc gatcacggca cctaccccta cgtcacctcc
    53281 agcaactgtg ttgcgggcaa tgccgccgcg ggttcgggtg tcgggcccgg gatgctgcat
    53341 tacgtgctgg gtatcaccaa agcctactgc acacgggtgg gaggcggccc gttcccgacc
    53401 gagctggaat gggagaagcc aggcacgccg ggctatcaca tgagcaccgt gggtgccgag
    53461 aagggcgtta ccaccggccg cagtcgccgc tgcggctggt ttgatgccgc gctgctcaag
    53521 cgcagtgcac aggtcaacgg cctgtcaggg ctatgcatca ccaagctgga tgtgctcgac
    53581 ggcctggaag acttgatgtt gtgtgtcggc tatgacctcg acggcgaacg catcgacctg
    53641 cttcccatgg gggcggacga cattactcgc tgcaagccca tctacgaaaa aatgccaggg
    53701 tgggcggatt cgacggtggg cgtgacgagc tatgacgcct tgccagtcca ggcgcgcaac
    53761 tatctggagc gtattgaaca ggtgacgggc gtgcccatcg cgatgatttc gacgagcccc
    53821 gatcgggatc acaccatttt ggtgcaaaac ccctacgctg ccggctagga agttcacatt
    53881 caatcgggct atgacgcccg tgaataaagc gacatgtgat atgaaaagag gagcattaaa
    53941 tgctgactga agacggcaaa cacctttacg taagctacga cgagtaccac aacctgatcg
    54001 aaaagctggc gttgaaagtg catcagtccg gctgggagtt cgacaccatt ttgtgcctcg
    54061 cccggggcgg cctgcgtccc ggcgacatcc tgagccgcat ctttcaaaaa cccctggcca
    54121 tcatgtctac cagttcctat cgtgccgagg ctgggaccgt tcaggggcat ctggatcttg
    54181 cgcgcttcat caccacaccc aagggggaaa ttgcggggcg cgtgctcctg gtcgacgatt
    54241 tggcggattc ggggcatacc ttgagggcgg tgattgacat gctcaagacc aactatgcac
    54301 ccatcaccga actgcgtagc gcggtgatct ggaccaaagg aatgtccacc ttcacgccgg
    54361 actactcagt ggaattcttg ccaaccaacc cgtggattca tcaacccttc gagggctacg
    54421 actccctggt tccggaaaaa ttggcagaaa aatggcgttg gtaggcgatc gcattgaaaa
    54481 ctttccgtac gggccttttt tgcggctata ctagcgggct cgctgcagcg aaagt
//
LOCUS       DAOMTQ010000005        46448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_33_length_46448_cov_27.818917, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000005 DAOMTQ010000000
VERSION     DAOMTQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 46448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46448
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33_length_46448_cov_27.818917"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PK518_01210"
     CDS             complement(<1..638)
                     /locus_tag="PK518_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517972.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM47557.1"
                     /translation="MSDYYLAILQGSLLTVGVSLASLAVATLLGLLGAAAKLSGSRPL
                     VWLAQLYTTVVRGVPELLMMLLIFYGGAIGLNHLLELWGNQEGVDLDPFVAGVLTIGF
                     IYGAYMTETFRGAILAIPRGQMEAAWAFGMGRLQTFVRITLPQMVRYALPSFTNNWLV
                     LIKATALVSLIGLHDMTYLAKQSSAAVREPFAFFLFAAALYLVFTSLSLWALR"
     gene            complement(705..1466)
                     /locus_tag="PK518_01215"
     CDS             complement(705..1466)
                     /locus_tag="PK518_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM47558.1"
                     /translation="MRKSIIALALAGLAGAAVAADLRVGLNPAYEPFESKTPTGEIVG
                     FDVDIANALCEQIKRKCVFVESEWDSIIPGLQAKKFDVIVSSMSITPERARVVDFTKR
                     YYKTPSAIVVKKSVKYDGPASLKGMKIGVLKASTQEKWALGELKPAGVTVVPYQSQNQ
                     VYLDIQAGRIDGTVADKVEVNGGFLRKPEGKDFGYVGADQYDVKYYGEGIGIALRKGQ
                     GDLKQQLNDAIDTIRANGTYEKIAKKYFDFDPYGK"
     gene            complement(1552..2538)
                     /locus_tag="PK518_01220"
     CDS             complement(1552..2538)
                     /locus_tag="PK518_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRM47559.1"
                     /translation="MSELHKFLFDGLPVRGMVVRLTDAWTELLARRAGNTETGPYPVP
                     VSELLGEMAAAAVLMQSNIKFNGALVLQIFGDGPVKLAVAEVQSDLSLRATASVTGEL
                     PADARLPDMVNVGGGGRCAITLDPKDRLPGQQPYQGVVPLHGDHKEKLTRLSDVLQHY
                     MLQSEQLDTTLVLAANDQVAAGLLIQRMPLKGEGNLAGAAGPADEDQIGQNEDYNRIA
                     TLAASLTRDELLSLDVDTILHRLFWEEKLLRFVPQQGADGPRFACSCSRARVGSMLRS
                     LGEGEVQSILDERGTIDVGCEYCGQQYHFDAVDAVQLFTAPGQQPPVSSSVQ"
     gene            complement(2548..3072)
                     /locus_tag="PK518_01225"
     CDS             complement(2548..3072)
                     /locus_tag="PK518_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRM47560.1"
                     /translation="MAIYELDGVAPQVAASAWVADSAQVIGDVVLGEGASVWFGTVVR
                     GDADRITIGAGTNVQDASVLHADIGVPLVIGNNVTVGHQVMLHGCTIGDESLIGIGAV
                     VLNGARIGRNCLVGAGSLVTEGKEFPDGSMILGSPARVVRQLSPEQIEGLRTSARHYM
                     ENARRFKAGLRQLD"
     gene            3113..3919
                     /locus_tag="PK518_01230"
     CDS             3113..3919
                     /locus_tag="PK518_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRM47561.1"
                     /translation="MELRQHALQVLCLTDPEQKSAAALDMKAQAATFLIATDAVIKAP
                     EAQALPGRPERPELRHHTAVARRSPATAEGRAVLIHAIAHIEFNAINLALDAIWRFPG
                     MPEQFYLDWLRVAAEEAKHFRLLRDHLRQHLGHDYGDFSAHQGLWSMCEKTAQDITAR
                     MALVPRTLEARGLDATPLIQHKLRSVGTSDALAAVAILDIILAEEVGHVAIGNHWYGW
                     LCARQGLDPVAHYAELVARHEAPRPKPPLNAAARRAAGFTEAELLWLQQG"
     gene            3987..5261
                     /locus_tag="PK518_01235"
     CDS             3987..5261
                     /locus_tag="PK518_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRM47562.1"
                     /translation="MSNTATAPTSSSPATPAPGGGVGAMIARQAIVDGQQSVVGYELF
                     NRSRSQVEHTAASDVLLVFTALSHAGTEELIGKVLLFVNCTHESLSGGHLELVNPDKV
                     VLEIPPLGHAAMEEVQARLPILAALRERGFHLAFNHQVLESAYAPWLPLADFIKLDIS
                     LLPPEQLAVLVKYAGRQSQARLIAEKVETAQQFDMVSALGVQLFQGYLFARPTVVQTR
                     LLSPGQASIVQLLNLLRGQASTDAIEEVLKKDAGLAFNLMRLINSASLGLTREITSIR
                     QAVLLLGLKKLFRWAALLLTAARSAGTPPATGQTAVVRGRLMELLANEFMSPEEADQA
                     FVIGVFSMLDQMLGMPQASAVGLLNLPDELSTALLERSNMHGRLLNLVEACESGDDDS
                     FDQAAQALPLTSQQINWAHLQALAWADAITQA"
     gene            5321..6574
                     /locus_tag="PK518_01240"
     CDS             5321..6574
                     /locus_tag="PK518_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRM47563.1"
                     /translation="MTAAEPGAEDADNQVVIARQAILDENRAVFGYELFDRSTATHSH
                     TAASDAALLFNALCYAGSEALGSKKIVFINCTHESLAGGHLELVHPEKVVLEVPTLAA
                     NATPEQIEQLLPTLQALRTRGFRLAFSQDVLRRAYGSWLPLANFIKLDMLAIKPELIE
                     PLVKYVRANAQATLIAEKVETAEQHERMAALGVKLFQGYWFARPAMVKAQTIRPSQAT
                     IIQLINLVRQQASTDEIEDLLKKDPTLSFNLLRFINSSGFGLSCEITSFRHAVMILGL
                     KKLFRWAALLLTTSRKDGTAPAVGQTAVVRGRLMELLAAELLPKEECDNAFVVGVFSM
                     LDSMLNMPMERALESVALPDSVQQALLHNQGVFAPFLELTKACESGDDAAFARCAEAL
                     HLSNRQVNWAHLQALAWAESLATEA"
     gene            complement(6586..7485)
                     /gene="xerD"
                     /locus_tag="PK518_01245"
     CDS             complement(6586..7485)
                     /gene="xerD"
                     /locus_tag="PK518_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRM47564.1"
                     /translation="MLPDSQTAIDLFLDALWLEDGLARNTLAAYRRDLTLYAQWLAQQ
                     QPPLVLDATAEHHLQGYFAARHAQTRATTANRRLTVLRRYFHWALRERRITQDPTVRL
                     LAARQPLRVPGTLTQAQVEALLHAPAPSTPLGLRDRAMLELMYASGLRVSELVTLKTF
                     HLGLADGVLRIMGKGGKERLVPFGDEARRWLERYLLEARGVILGGQQTDDLFVTQRGS
                     AMSRVMFWIIVKKCAQLAGITAPLSPHTLRHAFATHLLNHGADLRVVQLLLGHADIST
                     TTIYTHVARERLKQLHAQHHPRG"
     gene            complement(7533..8495)
                     /locus_tag="PK518_01250"
     CDS             complement(7533..8495)
                     /locus_tag="PK518_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugE"
                     /protein_id="HRM47565.1"
                     /translation="MQRRNYLALVLLATAGTAFAQAYPAKPIKLLVPFAPGGTTDIIA
                     RAIADPLGKVLGQPVVVDNRGGGGGIIGAQETARAAPDGYNLGIATVSTTATAPAINP
                     KVPYNALTDFTPVINVAATPNVIAVNPKFPAKDYQSFVAELKKNPGRYSYASSGTGTI
                     QHLLMELYKNLSGTFMTHIPYRGAGPALNDVVANQVSMNLDNLPSSLPFIKDGRLVPI
                     VVAAPQRLAVLPNVPTFKEVGLEPVNRLAFYGIVGPKGMPKEVVDKINAGVKKVLEDP
                     VVKKRIEDTGSFIVANTPEQFAEQIKAENAVYKEVVQKQKLTLE"
     gene            8607..9524
                     /locus_tag="PK518_01255"
     CDS             8607..9524
                     /locus_tag="PK518_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM47566.1"
                     /translation="MLRAVNYAQLLIPDFSLILCGYLVCRYTPLNRTVWQPVESLVYY
                     LLFPVLLFQSIVKSPIDVGEATGLVVAGVGTLLCGVALAYSLPHLPWLGRHIDRRDHA
                     AAAQVSFRFNSYLVLALAERLAGAQGLLMVAVLIGVCVPLVNVAAVWPMARGGQRGFW
                     GELLRNPLIIATATGLVANALGLRIPAWLEPAVSRIGAASLALGLMAAGAGMQFGLLR
                     RGKLLSASVLSIRHVALPLIAWAWVLLLGLDATQAQGLMVFCAVSTASSCYVLTARMG
                     YNGAYVAGLVTLSTALGVLSLPFGLGLLR"
     gene            complement(9566..10642)
                     /gene="queG"
                     /locus_tag="PK518_01260"
     CDS             complement(9566..10642)
                     /gene="queG"
                     /locus_tag="PK518_01260"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRM47567.1"
                     /translation="MVSSSQLVPQIRGWARELGFSQIGVAGVDLSAAEPGLMQWLAEG
                     FHGEMHYMAAHGLRRARPAELVPGTVSVITARMDYLPRATPPGWQAVEFDRLTRPREA
                     IVSVYARGRDYHKVLRARLQKLSERIAGAVGPFGHRVFTDSAPVLEAELARRSGQGWR
                     GKHTLVLSREAGSMFFLGEIYLDMALAPTQPVTAHCGSCQACMDVCPTQAIVAPHRVD
                     ARRCISYLTIEYAGPIPEELRPLMGNRIYGCDDCQLVCPWNKYAQASSLPDFDARAPL
                     VGQQLVQLFAWDEATFLRLTEGGPIRRIGHERWLRNIAVALGNALRATGDERLRAALL
                     TRADDPSALVREHVAWALLEEKRP"
     gene            10661..11134
                     /gene="tsaE"
                     /locus_tag="PK518_01265"
     CDS             10661..11134
                     /gene="tsaE"
                     /locus_tag="PK518_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRM47568.1"
                     /translation="MPPPLSLHWQGEDDAARFAARLAAQPGIANAFIALHGDLGAGKT
                     TLVRHLLRALGVAGRIKSPTYAVVEPHQAPGLAIWHFDFYRFNDPREWEDAGFRDLFA
                     SPGLKLAEWPNNASGLIPVADIAIYIEAEDDHARHVTLQAHTPQGSAVLQGLNTP"
     gene            11131..>11568
                     /locus_tag="PK518_01270"
     CDS             11131..>11568
                     /locus_tag="PK518_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HRM47569.1"
                     /translation="MSDERPPRPHHSRRRLLQAGSLVLLLGAQQIARGASIVAVRVWP
                     AADYSRVTIESDRALVAKQFFVSTPPRLAVDIEGLDLDPSLRELVAKVQPDDPNIAGI
                     RVGQNTPGVVRLVVDLKQAARPQVFTLAPVAAYQHRLVFDLYPA"
     assembly_gap    11569..11668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11669..12403
                     /locus_tag="PK518_01275"
     CDS             <11669..12403
                     /locus_tag="PK518_01275"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM47570.1"
                     /translation="ARSTDRLIIVALDPGHGGEDPGAIGPGGTREKDVVLKVARQLRD
                     RINATQVGGNPMRAFLTRDGDFFVPLGTRVEKARRVQADLFVSIHADAFTTPSARGAS
                     VFALSQGGASSSAARWLASKENEADLIGGVNVGSQDRHVQRALLDMSTTAQINDSLKL
                     GSVLLGEIGGMARLHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEEEAKLRSAAYQE
                     QLADALMRGIVRYFAKNPPLARSRTV"
     gene            12585..14165
                     /gene="xdhA"
                     /locus_tag="PK518_01280"
     CDS             12585..14165
                     /gene="xdhA"
                     /locus_tag="PK518_01280"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRM47571.1"
                     /translation="MTYATPSDHSRAIRFYHRGEIVSVSGVQTTRSVLDWLREDARHT
                     GTKEGCNEGDCGACTVVVGELDAAGQLQLASVNACIQFLPTLDGKALFTVEDLSQLAP
                     AGASCCGGACGSGTQPQLHPVQQALVDCHGSQCGFCTPGIAMTLWNSYEAHQSAGTRP
                     TRQQLADALSGNLCRCTGYRPILDAGERMFDLPDARLDRAAVTAALQELAKAPALAYS
                     APNPAASPARSDRFHAPRTLDDLAALRLALPQARLLAGSTDIGLWVNKQFRDVGELIY
                     VGQVAELRTVEERGDQLWIGAGASLEDAWRALAARAPTLTDCWLRFASTPIRHAGTMG
                     GNVANGSPIGDAPPILMTLDAAVELRRGAAVRRLALTDFYLDYMKNALQEGEFVQAIT
                     VPLAAFARQIRGYKISKRFDCDISALCAGMAMELDGDTVASVRLAYGGMAATVRRAAQ
                     AEAALLGQRWDEAAVRAAQAALARDFAPLSDMRASSAYRLQVAQNLIQRLWLETRATN
                     PLGSDATSVFSVMPHAAA"
     gene            14177..16606
                     /gene="xdhB"
                     /locus_tag="PK518_01285"
     CDS             14177..16606
                     /gene="xdhB"
                     /locus_tag="PK518_01285"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRM47572.1"
                     /translation="MNQPISSQLLARPVAFAPYQDNHGLRLDPAAEARAHAAGARVGI
                     SQAHESAQLHVTGSATYTDDIPELAGTLHCALGLAPVAAGRLLSIDLALLAAQPGVVR
                     VFTAADIPGSNDWGSIVHDDPILCDGDIRYLGQPVFAVVAESREQARRAAALAKKLIQ
                     AEAATPVLTPQDAHAKGQYVVPPMHLVRSSSGLDEAGIQARIQAAPHRLQGQLDVGGQ
                     EQFYLEGQISYAIPKEAGGMHVHCSTQHPSEMQHLVAHALGVAAHAVLVECRRMGGGF
                     GGKESQSALFACMASVAAQQLQRPVKLRLDRDDDFMITGRRHCFWYEYDVGYDDAGRI
                     LGAAVTMVSRAGHSADLSAPVMTRALCHFDNAYWLPEVAMHGFCGKTNTQSNTAFRGF
                     GGPQGAIAIEYILDSIARQLQQDPLAVRRANFYGTATHNITPYLQPVRDNVIHELVDQ
                     LEERSSYQARRAAIAAFNATSPVLKRGLALTPVKFGISFNVKHFNQAGALVHVYTDGS
                     ILVNHGGTEMGQGLNTKVAQVVAHELGVDFARVRATATDTSKVANTSATAASTGTDLN
                     GKAAQDAARQIRERLAACAAARHGGAAEDVRFANGQVTVAGKTISFETLVGEAYLDRV
                     QLWSDGFYATPGLSWDREKMQGNPFYYFAYGAAVSEVVVDTLTGEWKLLRADVLHDVG
                     RSLNPAVDIGQVEGAFIQGMGWLTTEELVWHPQTGLLMTHAPSTYKIPTANDCPPEFH
                     VQLFEGANAMDSIHRSKAVGEPPLLLPFSVFFAIRDAVAAAGGHQALPPLQAPATSEA
                     ILRAIGAVQAG"
     gene            16618..17613
                     /locus_tag="PK518_01290"
     CDS             16618..17613
                     /locus_tag="PK518_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM47573.1"
                     /translation="MPPSTPPTAPAGAGDQHLFDRIDLHLIRVLHTVLTERSVSRAAL
                     RLGMHQPAVSTALKRLRELSGDPLLVRNGAHMVPTEAGQRMLEPAANVLRAAETLFTE
                     ARSFDPRTSCQTFHIAASDYLDPLFLPRLVADIKRQAPLARVEIHALTREADYRAQLA
                     LGEIDLVIGNWLQPPDELHLARLFEDEVVSLVSRHHPAVRRGWDQDAWLAAEHIAPMA
                     THPGARGIIDQMLDGLGLKRHIAVRSAYFSLIPDMVAGTQLVLTTGRRYCERVASRLP
                     LVILPCPVALAPLQYYQLWHARSQHSSAARWLRERTRDAIAGLQLQPTTPLSPLS"
     gene            17665..18996
                     /locus_tag="PK518_01295"
     CDS             17665..18996
                     /locus_tag="PK518_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRM47574.1"
                     /translation="MPTTPPQAAATGLLERLFKLREHGTTPRTEVIAGITTFLTMAYI
                     IFVNPSILGDAGMPKDAVFVATCLIAALGSAIMGLYANYPIAMAPGMGLNAYFAYAVV
                     LHMGFTWQAALGAVFISGCLFLVVTLFGIRRHIIDGIPQSLRSAITVGIGLFLALIAL
                     KSAGIVVGDKATLVTLGDLHSAPVILAVLGFLAIVALDKARVPGAILIGIVGVTVASF
                     FFGGNQFRGIFSAPPSIAPTFLQLDIHTAVTKGLLNVVLVFFLVELFDATGTLMGVAK
                     RAGLLVPGKMERLNKSLLADSGAIFAGSLLGTSSTTAYIESASGVQAGGRTGMTALTV
                     AVLFLACLFIAPLAGVVPAYATAPALFFVACLMLRELIDLNWDDTTETVPAAVTALAM
                     PFTYSIANGLAFGFITYAAAKLFTGRVREVHFMVWIIAAVFLFKFFYIGGH"
     gene            19001..20281
                     /gene="guaD"
                     /locus_tag="PK518_01300"
     CDS             19001..20281
                     /gene="guaD"
                     /locus_tag="PK518_01300"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HRM47575.1"
                     /translation="MSPHAFRAQLLRFDADGQPLYDEDGLLVTATDAQGVRRVLDAGP
                     FGALRERHAQVPLTHWPGRLIAPGFIDMHVHYPQLDVIGAPADGLLPWLEHYTFPAES
                     RFADPEHAAEVAGFFFDELQRNGVTTALTFATSHPASVDAFFGQAQRRGLRMIGGKVL
                     QDRHSPDGVRDATEQSLIDTEALIQRWHGQGRLGYAITPRFAPSCSDAQMHGAAELAR
                     RHPDTWIQSHVAENRDEVRWVRELYPQARSYLDVYAGFGLLRRRAVYAHCIYLDDTDR
                     QLMHETGTAAAVSPTSNLFLGSGFFNFIGADRLGMLHGLASDVGGGTSFSPFHTMLAA
                     YYVGREGQTKPGLSLKPSQLWWQHTGGAARALGLEGVIGTLQAGTEADFVVLNPQATP
                     LLARKTALAGSLEELLFALIVLGDDRLVERVVIA"
     gene            complement(20278..20922)
                     /locus_tag="PK518_01305"
     CDS             complement(20278..20922)
                     /locus_tag="PK518_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRM47576.1"
                     /translation="MDIIAFLIDFILHVDKHLEAFVASYGAWVYALLFLIVFVETGVV
                     VMPFLPGDSLLFIVGALSGAGLLNFPLACTLLVVAAILGDQCNYSIGRYLGPKVFQWE
                     GSRWFNKKAFDQAHAFYERYGGITIVLARFMPFIRTFAPFVAGVAAMGRAKFTAYNVA
                     GALIWVLGIATAGYFFGNLPWVRQHLEKIIWGLILVPGLIAIFGAWRAGRQQPA"
     gene            21216..21482
                     /locus_tag="PK518_01310"
     CDS             21216..21482
                     /locus_tag="PK518_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HRM47577.1"
                     /translation="MIFRRTHTVRALTVATALVGALALAGCAHHPSNAQIGTGVGAVA
                     GGLVGNAVFGSTLGTVGGAAAGALIGNEVGKNNDRGRGRRGHRH"
     gene            21529..23436
                     /gene="mutL"
                     /locus_tag="PK518_01315"
     CDS             21529..23436
                     /gene="mutL"
                     /locus_tag="PK518_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRM47578.1"
                     /translation="MTAQPSDPATPSPRRPIRDLPDELISQIAAGEVVERPASAVREL
                     VDNALDAGARQITVRLAAGGVRLIAVEDDGCGIPRDELPVALRRHATSKITNLHDLES
                     VATMGFRGEALAAIASVSEMALLSRTAQQASAFALDARSGELRPAARSTGTTVEVKEL
                     FFSTPARRKFLKTDATELAHCVEAVRRHALARPDVGFAIWHEGRLVEQWRTTLAPGVD
                     ADAALQQRLGDVLGDDFLRNSVPVQLRLGAVTVTGRAGLPDAARSRADQQFCYVNGRF
                     VRDKVLTHAARSAYEDVLHGQKQPVYALYVQIDPQRVDVNVHPTKIEVRFRESREVHQ
                     AVRHAVENALAAPRAQALAAASATPAVAPPGDFEQNRPVAPNWQAQTAIKFDERMGHK
                     VSDLQALWGTPAAQPAPPGPTMEPAGATSPAAAMPLPAAVETPADAANTWPLGRALAQ
                     LHGIYILAESAQGLVLVDMHAAHERIVYERLKSQVDQGARIASQPLLIPATFAATPEE
                     VATAEAHADTLGLLGLEVVPFSPRTLAVRAVPTQLAQGNAAELARSVLAELGQHDAST
                     VIQRARNEILATMACHGAVRANRRLTLDEMNALLRQMEVTERSDQCNHGRPTWRQLTM
                     KELDGLFLRGR"
     gene            complement(23468..24604)
                     /locus_tag="PK518_01320"
     CDS             complement(23468..24604)
                     /locus_tag="PK518_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRM47579.1"
                     /translation="MAAAAVLLAACGGGDDKGGYPTLTGDKPLVIGHRGASGYLPDHT
                     LEGYRKAIELGADFIEPDLVATKDGVLVARHEPNITATTDVAQRSEFAARKSKKLVDG
                     VSEEGWFVSDFTLAELKTLRAVQPLAERDQSHNGKYLIPTFEEVLELAKTEGARLNRS
                     IGVYPETKHPSYHAGLGLALEDRLLAILGKYGYTSKSAPVIVQSFEVANLKYLRSKTQ
                     VRLVQLVDANDVNADGSMDLTAPYDKPYDFAVAGDARTFASLLTPAGLKDVKSYADGI
                     GPWKPYLIPSKLVDANKDGKPDDLNGDGKIDERDRVMLPATDVVKNAHAAGLFVHPYT
                     FRSEARRLASDFKGDPKAEFRLFYQLGVDGVFADFPDHAKAARD"
     gene            24868..26211
                     /gene="gdhA"
                     /locus_tag="PK518_01325"
     CDS             24868..26211
                     /gene="gdhA"
                     /locus_tag="PK518_01325"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRM47580.1"
                     /translation="MKHASVGQFLEQLAQRNPGQPEFLQAVTEVMESLWPFIEKHPRY
                     AEQALLERLVEPERVIMFRVSWFDDHGNVHVNRGYRIQHSMAVGPFKGGIRFHPSVTL
                     SVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSPTEIMRFCQAFVTELFRHVGPD
                     TDVPAGDIGVGGREVGFMAGMYKKLANNAASVFTGKGLSFGGSLIRPEATGYGCVYFA
                     QEMLATRGQSLEGKTVSVSGSGNVAQYSVEKAMQLGAKVVTVSDSSGTIYDPEGFTAE
                     KLAILMDVKNHHYGRVNDYAKRTGVEFLPGKTPWHIPADVALPSATQNELDEQDARTL
                     IKNGVLCVAEGANMPSTNEAAKVFETAGVLYAPGKASNAGGVATSGLEMSQNAQRLSW
                     TRDEVDARLLQIMKSIHGACLQHGKRADGSVSYIDGANIAGFVKVADAMLAQGVV"
     gene            complement(26213..26827)
                     /locus_tag="PK518_01330"
     CDS             complement(26213..26827)
                     /locus_tag="PK518_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM47581.1"
                     /translation="MLHVQGLGFGFPGHSLFQDWSADFPAGLSLIQGGDGTGKTSLLR
                     LFAGETAPQAGSVTLLGMDAVRQPAEYRAQVFWRDPRAPWPDTLTPLTWVEQLPAHHP
                     RWSGEAWRAHVAGFGLAMHLHKPMYQLSSGSQRKVLLAAALASGAVLTLLDEPLAALD
                     KPSISYLLQALEAEARAPRWPGRAVLVAHYDALGDLPWCARIAL"
     gene            26908..27276
                     /locus_tag="PK518_01335"
     CDS             26908..27276
                     /locus_tag="PK518_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47582.1"
                     /translation="MKLHFLDFDYSEDAEGTATWDAIAAIPAARMSELEHEIISVLAW
                     AHAEYGELRGPLESGGLWDYDLQCERDGHPLLAMSYNTETRRLEPLPQLPPDARVALT
                     LSLCGGQAFDEAFAARFGAA"
     gene            complement(27301..27768)
                     /locus_tag="PK518_01340"
     CDS             complement(27301..27768)
                     /locus_tag="PK518_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRM47583.1"
                     /translation="MKVLDLFCADDHAFEGWFGSEDDFQSQLARGLVQCPLCGNAHIR
                     KGLSAPRLNLRSSSDAGSQASQPTQPVQATNGAVTPQAMHAAWLHMARQIVARTEDVG
                     QRFAVEARRMHYGEIEERPIRGQASPGETAELLDEGIAVLPLLLPDAATESLQ"
     gene            complement(27780..28097)
                     /locus_tag="PK518_01345"
     CDS             complement(27780..28097)
                     /locus_tag="PK518_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HRM47584.1"
                     /translation="MTQTAAIWLVILAALVAANLPFINDRWLVVGARAHQGKPFFARA
                     LELLLLYFLVGALALLIEKRAGQIAPQGWEFYAVTGALFITLAFPGFVYRYLMHRGRR
                     PAH"
     gene            complement(28117..29610)
                     /gene="nuoN"
                     /locus_tag="PK518_01350"
     CDS             complement(28117..29610)
                     /gene="nuoN"
                     /locus_tag="PK518_01350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRM47585.1"
                     /translation="MIDNISWLAIYPEIVLLVMACVIALVDLGVGSTRRTATYVLTML
                     SLAVVAVMQAMYASSGNTFYGWGNMVVSDAMGNWLKCFATVSLMVTLVYGRSYALDRG
                     MLRGGELFTLTMLALLGICVLISANNFIVIYLGLELLTLSSYALVALRRDHTTATEAA
                     MKYFVLGAMASGFLLYGLSMVYGATGSLDIGQVFKAINAGQIRHQVLVFGVVFIVAGL
                     AFKFGAVPFHMWIPDVYQGAPTVVTLIIGSAPKFAAFAMAIRLLVDGLLPLAIDWQQM
                     LAVLAIASLLVGNVAGVLQTNLKRMLAYSTISHMGFLFLGLLSGVVNGMVDANAAETA
                     YSSAMFYIVTYVLTVLPAFGLIAMLAREGFESEEIADLAGLNQRSPLYAAVMAVCMFS
                     LAGIPPLVGFYAKLSVLQALVASGHGLHVALAVFAVVMSLIGAFYYLRVVKVMYFDAP
                     LTAGKVSAPFDARVVLSINGALVLILGVLPGGLMALCADAIVRALAT"
     gene            complement(29638..31113)
                     /locus_tag="PK518_01355"
     CDS             complement(29638..31113)
                     /locus_tag="PK518_01355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRM47586.1"
                     /translation="MGLLSLAIWTPIVFGVLLLAFGCDKHARTVRWLALIGSLLGLAV
                     TIPLISGFDAGTAAMQFVEKAPWVARFNMHYHLGVDGISMWFVPLTAFITVIVVIASW
                     ESITERVYQYMAAFLILSGLMIGVFSALDGMLFYVFFEATLIPMYLIIGMWGGPNKIY
                     AAFKFFLYTLLGSLLTLVAFIYLYNQAGGSFDIAAWHKLPLGGTAQTLLFFALFAAFA
                     VKVPMWPVHTWLPDVHVEAPTGGSAVLAAIMLKLGAYGFLRFSLPIAPDAAHEWAWLM
                     ITLSLIAVIYVGLVAIVQKDMKKLVAYSSVAHMGFVTLGFFIFNDLGVSGGLAQMIAH
                     GFVSGAMFLCIGVLYDRVHSRQIADYGGVVNTMPKFAAFALLFSMANCGLPATAGFVG
                     EWMVIIASVRFNFWIGLGAAIAVILGASYSLWMYKRVYLGPVTNDNVRAMTDLNGREF
                     LVLGVLAIAVLYMGIYPKPFTDVMDVSVAELLKHVAQTKLN"
     gene            complement(31127..33157)
                     /gene="nuoL"
                     /locus_tag="PK518_01360"
     CDS             complement(31127..33157)
                     /gene="nuoL"
                     /locus_tag="PK518_01360"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRM47587.1"
                     /translation="MSQTLSASTLLAVPLAPLAGSLLAGLFGTAFGGNWFGRRISHTL
                     TILGVLVSFLISASTLYSVAVDGARLNETLYTWMVVGGLKMEVGFLIDGLTAMMMCVV
                     TFVSLMVHIYTIGYMEEDEGYNRFFSYISLFTFSMLMLVMSNNMLQLFFGWEAVGLVS
                     YLLIGFWFNKPTAIFANMKAFLVNRVGDFGFILGIGLIAAYAGSFDYAEVFAKKQELG
                     ALVFPGTDWMLITVICICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTA
                     GIFMVSRMSPLFELSDAALNFILVIGSITALFMGFLGIIQNDIKRVVAYSTLSQLGYM
                     TVALGASAYSVAVFHLMTHAFFKALLFLGAGSVIMGMHHNQDIRWMGGVRKYMPITWI
                     TSLLGSLALIGTPLFSGFYSKENIIEAVHFSQLPAAGFAHFAVMAGVFVTAFYSFRMY
                     FLVFHGKERYDQNPDAHEAQEEPDPHHHHDDHSKPHESPWVVTVPLVLLAIPSVVIGF
                     MYIQPMLFGDFFKDVIFVDGSKHAAMAQLAQMFHGPVAMAVHALQTAPFWLALAGVAL
                     SYYMYIVNPALPAAIKRVFQPIYTLLENKYYMDWINENIIAAGTRLLGVVLWKGGDQG
                     LIDGAIVNGSWKLVARISAAVRRVQSGFLYHYALFMILGVFALMTYFVWLNK"
     gene            complement(33190..33498)
                     /gene="nuoK"
                     /locus_tag="PK518_01365"
     CDS             complement(33190..33498)
                     /gene="nuoK"
                     /locus_tag="PK518_01365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRM47588.1"
                     /translation="MTLTLGHFLTLGAMLFALAVVGIFLNRKNLIVLLMAIELMLLAV
                     NMNFVAFSHYLGDMHGQVFVFFILTVAAAEAAIGLAILVLLFRNKNSINAEDLNTLKG
                     "
     gene            complement(33495..34163)
                     /locus_tag="PK518_01370"
     CDS             complement(33495..34163)
                     /locus_tag="PK518_01370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRM47589.1"
                     /translation="MDVKTGFFYLFSVVLLYAAFRVITARNPVHAVLHLILAFSQAAG
                     LWLLLKAEFLAIALVLVYLGAVMVLFLFVVMMLDIRIDAVRRGFWKHFPLAALIGALV
                     AFEMGAVLMTGFRGVEEPKAVATLVNAAGQVVPYSNTQALGKLMYTEYLYPVEIAAVI
                     LLVAMISAIALTLRQRKDVKASDVTAQVRARASDRLVVVKMPVTQAAQPLADGDISAE
                     ENKA"
     gene            complement(34207..34719)
                     /gene="nuoI"
                     /locus_tag="PK518_01375"
     CDS             complement(34207..34719)
                     /gene="nuoI"
                     /locus_tag="PK518_01375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRM47590.1"
                     /translation="MSAVVAPASFSLKDFFKSFMLWELAKGMVLTGRYAFRGKVTVQF
                     PEEKTPLSPRFRGLHALRRYDNGEERCIACKLCEAVCPAMAITIESDVRADGTRRTTR
                     YDIDLTKCIFCGFCEESCPVDSIVETQILEYHGEKRGDLYFTKEMLLAVGDRYEPEIA
                     AAKAADAKYR"
     gene            complement(34735..35811)
                     /gene="nuoH"
                     /locus_tag="PK518_01380"
     CDS             complement(34735..35811)
                     /gene="nuoH"
                     /locus_tag="PK518_01380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRM47591.1"
                     /translation="MIDAIYNGGLNLVAASWWTSMGWPVIWNLIKIVCVLAPLMGAVA
                     YLTLWERKLLGFMQARLGPNRVGPLGLLQPLADGLKLLTKEVIQPTAASKGLFFLGPV
                     MAIMPALAAWVVVPFAPDTALANINAGLLLVMAIASIEVYGVIIAGWASNSKYALLGA
                     LRASAQMVSYEIAMGFCFVVVIMVTGSMNLTEIVLGQARGTMAGIGLGFLSWNWLPLL
                     PIFLVYLISGVAETNRHPFDVVEGEAEIVAGHMVEYSGMGFAIFFLAEYAAMWLVSIL
                     AVIMFLGGWLPPVDALGFIPGWIWLGIKTFVVVSMFIWIRATFPRYRYDQIMRLGWKI
                     FIPVTLVWVLVVGAWLLSPWNIWK"
     gene            complement(35808..37928)
                     /gene="nuoG"
                     /locus_tag="PK518_01385"
     CDS             complement(35808..37928)
                     /gene="nuoG"
                     /locus_tag="PK518_01385"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRM47592.1"
                     /translation="MVEIELDGQKVEVAEGSMVMHAAEKSGTYIPHFCYHKKLSIAAN
                     CRMCLVDIEKAPKPMPACATPVTQGMIVRTKSDKAIKAQQSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASASRYGEEKRVVLKKDIGPLISTEEMTRCIHCTRCVRFGQEI
                     GGDMELGMINRGEHSEITTVKGDTVDSELSGNMIDICPVGALTSKPFRYSARTWELSR
                     RKSISPHDSTGANLVVQIKNHKVMRVVPLENEAVNECWIADRDRFSYEALNGDDRLQR
                     PMLKQGGQWQEVDWQTALEYVANGLKCVRDKHGASAIGALVSPHSTLEELHLAATLVR
                     GLGSDNIDHRLRHAEFATPEGVRWLGMPIAALTDLQSVLVLGSNLRKDHPLFAQRIRQ
                     AARKGCKVFAINAMAYDWALPATQSVVASADWTQALADVAAAVAQEKGTVAPVVSGKA
                     NDEALAIARALLSGERKAILLGNAAAHHAQASSLLALAQWIAQATGATFGYLSEAANT
                     VGAQFVGAQPRQGGLNAAQMLAGQVKALFLLNTEPEFDSAAGANAARGLADVDMVVTL
                     SPFKTNMDISDVLLPIAPFTETSGSFVNAEGRVQSFHAVVRPLGETRPAWKVLRVLAN
                     IMALPGVEFESSQDVLNQATAGLEQLTSQQLSNATKAAIDVAHGPAAAPVVASIYQLD
                     GLVRRAPALQLTADARAAREGVVA"
     gene            complement(37938..39299)
                     /gene="nuoF"
                     /locus_tag="PK518_01390"
     CDS             complement(37938..39299)
                     /gene="nuoF"
                     /locus_tag="PK518_01390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRM47593.1"
                     /translation="MTTVAQVLSQFQATGVQTCFHDRHINPQIYADLNGGNWSIKDYE
                     ARGGYAALRKILGKDGGEGLTQDQVIATVKESGLRGRGGAGFPTGLKWSFMPRQFPGQ
                     KHLVCNSDEGEPGTCKDRDILMFNPHIVIEGMIIAAYAMGISLGYNYIHGEIFQVYER
                     FEAALEEARAAGYLGNNIMGSAFSFQLHAHHGFGAYICGEETALLESLEGKKGQPRFK
                     PPFPASFGLYGKPTTINNTETFAAVPWIIRNGGQAYLECGKPNNGGTKIFSVSGDVER
                     PGNYEIPLGTPFAKLLELAGGVRKGRQLKAVIPGGSSSPVLPASIMMDCTMDYDSIAK
                     AGSMLGSGAVIVMDDSRDMVESLVRLSYFYMHESCGQCTPCREGTGWLWRVVHRIQHG
                     QGREEDIELLDNVAFNIMGRTICALGDAAAMPVRAMIKHFRHEFVAKIEAAVKQGTSA
                     ARV"
     gene            complement(39296..39787)
                     /gene="nuoE"
                     /locus_tag="PK518_01395"
     CDS             complement(39296..39787)
                     /gene="nuoE"
                     /locus_tag="PK518_01395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRM47594.1"
                     /translation="MITEATKARFAREVAKYPPEQKQSAVMACLSIVQQEQGWVSQEN
                     EAVIADYLGMPEIAVHEVTTFYNMYNQQPVGKYKLAVCTNLPCQLRGGNQALHHLEAK
                     LGIEMGETTPDGMFTLQQCECLGACADAPAMLVSDRHMCSFMDNDKLDQLVDGLRAAE
                     GQQ"
     gene            complement(39784..41037)
                     /locus_tag="PK518_01400"
     CDS             complement(39784..41037)
                     /locus_tag="PK518_01400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRM47595.1"
                     /translation="MADIKNYSLNFGPQHPAAHGVLRLVLELDGEVVQRADPHVGLLH
                     RATEKLAEHKTYIQSLPYMDRLDYVSMMCNEQAYCLAIEKLLGIEVPLRAKYIRTMFG
                     EITRLLNHLMWLGSNGMDLGASTVLMYTFREREVLFDMYEAVSGARMHAAYYRPGGVY
                     RDLPDSMAQLKPSKVRSAKATEQFNSNRQGSLLDYIEDFCAKFPNLVDEYETLLTENR
                     IWKQRTVGIGVVSAERALNLGMTGVMLRGSGVAWDLRKKQPYDAYDRVDFDVPLGKNG
                     DCYDRYLVRVEEMRQSNRIIQQCAKWLRANPGPVIVDNYKVAPPPRERMKTGMEDLIH
                     HFKLFSEGFKVPEGEAYAAVEHPKGEFGIYIVSDGANKPYRLKIRAPGFAHLSAYDEL
                     TRGHMIADAVAVIGTMDIVFGEIDR"
     gene            complement(41065..41673)
                     /locus_tag="PK518_01405"
     CDS             complement(41065..41673)
                     /locus_tag="PK518_01405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRM47596.1"
                     /translation="MTDFAIHPEKLREAVAAALGDKVRDIALALDELTVVVAAGDYLD
                     VMQTLRDAPQCRFEQLIDLCGVDYSTYREVGTDGLRYAVVSHLLSVSLNQRVRVKVFC
                     PDDDFPVIPSVTDIWSGANWFEREAFDLFGIVFDGHNDLRRILTDYGFIGHPFRKDFP
                     LSGHVEMRYDPEQRRVIYEPVTIEPREITPRIIREEKYGGLH"
     gene            complement(41683..42162)
                     /locus_tag="PK518_01410"
     CDS             complement(41683..42162)
                     /locus_tag="PK518_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRM47597.1"
                     /translation="MIEGVMKEGFVTTSYDAVVNWAKTGSIWPVTFGLACCAVEMMHA
                     AAARYDLARFGAEVFRASPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRVVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTQTIARV"
     gene            complement(42188..42547)
                     /locus_tag="PK518_01415"
     CDS             complement(42188..42547)
                     /locus_tag="PK518_01415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRM47598.1"
                     /translation="MNLDQYLPVLLFILVGVGVGVVPLVLGYVLGPNRPDPAKNSPYE
                     CGFEAFDDARMKFDVRYYLVAILFILFDLEIAFLFPWAVALQDVGMTGFVAVVIFLAI
                     LVIGFAYEWKKGALDWE"
     gene            complement(42619..42703)
                     /locus_tag="PK518_01420"
     tRNA            complement(42619..42703)
                     /locus_tag="PK518_01420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42667..42669),aa:Leu,seq:gag)
     gene            complement(42833..43207)
                     /gene="secG"
                     /locus_tag="PK518_01425"
     CDS             complement(42833..43207)
                     /gene="secG"
                     /locus_tag="PK518_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRM47599.1"
                     /translation="MNAIVNIILAVQMLTALGMIGLILIQHGKGADMGAAFGSGSAGS
                     LFGASGSANFLSRSTAALATVFFVATLALAYFGNQRAPSTGSVLETPAAAVPASSTAP
                     ADAAVPAPAAEPASGAAQIPTK"
     gene            complement(43237..43998)
                     /gene="tpiA"
                     /locus_tag="PK518_01430"
     CDS             complement(43237..43998)
                     /gene="tpiA"
                     /locus_tag="PK518_01430"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRM47600.1"
                     /translation="MTQIKKLIAGNWKMNGSLQANAALLQGLRGGLGQPACNVAVAVP
                     APYLAQVQALVAGSSIALAAQDVSQHESGAYTGETSAAMLRDFGARYVLVGHSERRQY
                     HGETDAVVAAKAQRALAAGITPIVCVGETLEQREAGQTEAVVKRQLAAVIQANGHCIS
                     EIVVAYEPVWAIGTGRTATPEQAQAVHAVLRAQLAAATEQAARIPLLYGGSMNAANAA
                     QLLAQPDIDGGLVGGASLKAEDFLQIIAAAQTNKA"
     gene            complement(43995..44987)
                     /locus_tag="PK518_01435"
     CDS             complement(43995..44987)
                     /locus_tag="PK518_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRM47601.1"
                     /translation="MQAVEITAFGAPEVLRLGERPVPMAGVGELLIRVAASGVNRPDV
                     LQRKGHYAPPAGASDLPGLEVAGVIESGDAAALAAAGFAVGDRVCALVAGGGYAEYCV
                     APVAQCLPVPAGLSDVEAASLPETFFTVWSNVFDRARLQAGETLLVQGGSSGIGVTAI
                     QLARARGATVIITAGSDEKCAACLALGAHHAINYQAQDFVAEVQHITEGRGVDVVLDM
                     VAGPYVAREVECLAEDGRIVIIAVQGGVKAEFNAALVLRKRLVITGSTLRPRPVAFKA
                     AIAQALREQVWPLLASGAIKPVIHSTFAAADAAQAHALMESNQHIGKIVLRWTT"
     gene            complement(45088..>46448)
                     /gene="pnp"
                     /locus_tag="PK518_01440"
     CDS             complement(45088..>46448)
                     /gene="pnp"
                     /locus_tag="PK518_01440"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRM47602.1"
                     /translation="DGVKVDNMLFDIEARIVRGQILAGEPRIDGRDTRTVRAIEIRNS
                     VLPRTHGSALFTRGETQALVVSTLGTERDAQRIDALAGEFEDRFLFHYNMPPFATGEV
                     GRMGSTKRREVGHGRLAKRALVACLPSKEEFPYTIRVVSEITESNGSSSMASVCGGCL
                     SMMDAGVPMKAHVAGIAMGLIKEDNKFAVLTDILGDEDHLGDMDFKVAGTTAGITALQ
                     MDIKIQGITKEIMQVALAQAKEARMHILAKMQEAMGEAKAEISSFAPKLFTMKINPEK
                     IRDVIGKGGATIRALCDETGCQINIEEDGTITIAATEAAKADEAKRRIEEITAEVEIG
                     KVYEGPITKILDFGALVNVLPGKDGLLHISQIAHERVEKVTDYLQEGQIVRVKALEAD
                     DKGRIKLSMKALIERPAGMPEREPREPREYRERAPRAPRENREPREPRAERGEQDQPT
                     QE"
BASE COUNT         8579 a        15542 c        14478 g         7749 t
ORIGIN      
        1 cgcagcgccc acagcgacag cgaggtgaac accagataca gggcggcggc aaacaggaag
       61 aaggcaaacg gctcgcgcac ggcggcactg gactgcttgg ccaggtaggt catgtcgtgc
      121 aggccgatca ggctcaccag cgccgtggcc ttgatcagca ccagccagtt gttggtgaag
      181 ctgggcagcg cgtagcgcac catctgcggc agcgtgatgc gcacaaaggt ctgcagtcgg
      241 cccatgccaa aggcccaggc ggcctccatc tggccgcgtg gaatggccag gatggcgccg
      301 cgaaaggtct cggtcatgta ggcgccgtag atgaagccaa tggtcagcac gccggcgacg
      361 aaggggtcga gatccacgcc ctcctggttg ccccacagct ccagcaggtg gttcaggccg
      421 atggcgccgc cgtaaaaaat cagcagcatc atcaacagct ccggcacgcc gcgcaccacg
      481 gtggtgtaga gctgggccag ccacaccagc ggccggcttc ccgagagctt ggccgccgcg
      541 cccagcagcc ccagcagcgt ggccacggcg agcgaggcca gcgacacgcc cacggtgagc
      601 agcgagcctt gcaggatggc caggtagtaa tcactcatgg ataaaaaacg gggcgttgcg
      661 cgccgagggc cgcaaggcac ccgacgccaa cgccccatcg ggctttactt gccgtaggga
      721 tcgaagtcga agtacttctt ggcgatcttc tcgtaggtgc cgttggcgcg aatggtgtcg
      781 atggcgtcgt tgagctgctg cttcaaatcg ccctggccct tgcgcagcgc aatgccaatg
      841 ccctcgccgt agtacttcac gtcgtactgg tcggcgccga catagccgaa gtccttgccc
      901 tcgggtttgc gcaggaagcc gccgttgacc tcgaccttgt cggccaccgt gccgtcgatg
      961 cggccggcct ggatgtccag gtagacctgg ttctgcgact ggtagggcac gacggtgacg
     1021 ccggcgggct tcaactcacc cagggcccat ttctcttgcg tgctggcctt gagcacgccg
     1081 atcttcatgc ccttgaggga tgccgggccg tcgtacttga cgctcttctt gaccacgatg
     1141 gcgctggggg tcttgtagta gcgcttggtg aagtccacca cgcgggcgcg ctcgggcgtg
     1201 atcgacatgg agctgacgat gacgtcgaac ttcttggcct gcaggccggg gatgatggag
     1261 tcccactcgg actcgacaaa cacgcacttg cgcttgatct gctcgcacag ggcgttggcg
     1321 atgtccacgt cgaagccgac gatctcgccc gtgggcgtct tggactcgaa gggttcgtag
     1381 gccgggttca ggccgacacg caggtcggcg gcaacggccg cgcccgccag gccagcgagc
     1441 gccagggcaa tgatgctttt gcgcatggta gtggttcctt ggaaagggaa ggcaaagcgc
     1501 gtattgtcgc cgctgcgcgc ggccacgccg gcgggacacc ccgccccgcc cttattgcac
     1561 ggacgacgag accggcggct gctggcccgg cgcggtgaac agctgcaccg cgtccacggc
     1621 gtcgaagtgg tattgctggc cacagtattc gcagcccacg tcgatggtgc cacgctcgtc
     1681 cagaatgctc tgcacctcgc cctcgcccag gctgcgcagc atgctgccca cgcgcgcgcg
     1741 gctgcacgag caggcaaagc gcggcccgtc cgccccctgc tggggcacga agcgcagcag
     1801 cttttcctcc cagaacaggc ggtgcaggat ggtgtccacg tccagactca atagctcgtc
     1861 acgcgtcagg ctggcggcca gcgtggcgat gcggttgtag tcctcgttct ggccgatctg
     1921 gtcttcgtcg gcagggccgg cggcgcccgc caggttgccc tcgcccttga gcggcatgcg
     1981 ctggatcagc aggccggcgg ccacctggtc gttggcggcc agcaccagcg tggtgtccag
     2041 ctgctcggac tgcaacatgt agtgctgcaa cacatcgctg agcctggtga gcttttcctt
     2101 gtggtcgcca tgcagcggca ccacgccctg atagggctgc tggccgggca ggcgatcctt
     2161 gggatcgagc gtgatggcgc agcgcccgcc gccgcccaca ttcaccatgt ccggcaggcg
     2221 cgcatcggcc ggcagctcgc cggtcaccga ggccgtcgcg cgcaggctca ggtcggactg
     2281 cacctcggcc acggccagct tcaccggccc gtcgccaaag atctgcagca ccagcgcgcc
     2341 gttgaacttg atgttggatt gcatcagcac ggcggcggcg gccatttcgc ccagaagctc
     2401 gctcacgggc accgggtacg gcccggtctc ggtattaccg gcgcggcgcg cgagcagctc
     2461 cgtccaggca tcggtcaggc gcacgaccat gccgcgcacg ggcaggccat caaacaaaaa
     2521 cttgtgcagt tcagacatcc agtcgcatca atccagttgg cgcaggcccg ctttgaagcg
     2581 gcgcgcgttt tccatatagt gccgcgcact ggtgcgcagc ccctcgatct gctcgggcga
     2641 gagctggcgc acgacgcgcg ccggactgcc caggatcatg gagccgtcgg gaaactcctt
     2701 tccctcggtg accagcgagc ccgcgcccac cagacagttc ctgccgatgc gcgcgccgtt
     2761 gagcaccacg gcgccaatgc cgatcagcga ctcgtcgcca atcgtgcagc catgcagcat
     2821 gacctggtgg cccaccgtca cgttgttgcc tatcaccagc ggcacgccga tgtcggcatg
     2881 cagcacgctg gcgtcctgca cgttggtgcc ggcgcctatg gtgatgcggt cggcgtcgcc
     2941 gcgcaccacg gtgccgaacc agacgctggc gccctcgccc agcaccacat cgcctatcac
     3001 ctgcgcgctg tcggccaccc aggccgatgc cgccacctgc ggcgcgacgc cgtcgagttc
     3061 atagatcgcc atggctgcct gcctccgtgg ttcaaaacct acaattgtag ggatggagct
     3121 tcgtcaacac gccctgcagg tcttatgcct cacagatcct gaacaaaaat cggccgcagc
     3181 gcttgatatg aaagcgcaag cagctacatt tttaatagct acagatgccg tcatcaaggc
     3241 gcccgaggcg caggccctgc ccggccggcc ggagcgcccc gagctgcgcc accacaccgc
     3301 cgtggcgcgc cgctcacccg ccacggccga gggccgcgcg gtgctgatcc atgccatcgc
     3361 gcacatcgag ttcaacgcca tcaacctggc gctggacgcc atctggcgct ttcccggcat
     3421 gcccgagcag ttctacctgg actggctgcg cgtggcggcc gaggaggcga agcactttcg
     3481 cctgctgcgt gaccacctgc gccagcatct gggccacgac tatggcgact tctccgcgca
     3541 ccagggcctg tggagcatgt gcgagaagac ggcgcaggac atcaccgcgc gcatggccct
     3601 ggtgccgcgc acactggagg cgcgcggcct ggacgccacg ccgctgatcc agcacaagct
     3661 gcgcagcgtg ggcaccagcg acgcgctggc ggccgtggcc atactggaca tcatccttgc
     3721 cgaggaagtg ggccatgtgg ccataggcaa ccattggtat ggctggttgt gcgcgcgtca
     3781 ggggctggac ccggtggcgc attacgccga gctggttgcg cgccacgagg cgccgcgccc
     3841 caagcccccc ttgaacgcgg ccgcacgccg cgccgccggc ttcaccgagg ccgagctgct
     3901 ctggctgcag cagggctgag caacaactta cttcagccga aatgggccaa ttacctacaa
     3961 tgagggatgc tctagccctt cctgccatgt ccaacaccgc caccgcccca acctcctcca
     4021 gcccggccac cccagcgccg ggtggcggcg tgggcgccat gattgcgcgc caggcgatcg
     4081 tggacggcca gcagagcgtc gtcggttacg aactgttcaa ccgctcacgc tctcaggtcg
     4141 agcacacggc cgccagcgat gtgctgctgg tgttcacggc gctatcgcat gccggcaccg
     4201 aggagctgat cggcaaggtg ctgctcttcg tcaactgcac gcatgagagc ctgagcggcg
     4261 gccacctgga gttggtgaac ccggacaagg tggtcctgga gatccccccg ctgggccacg
     4321 ccgcgatgga ggaggtgcag gcccggttgc cgattctggc ggcgctgcgc gaacggggct
     4381 ttcacctcgc gttcaaccac caggtgctgg agtccgccta cgccccctgg ctgccgctgg
     4441 ccgacttcat caagctcgac atcagcctgc tgccccccga gcagctggcg gtgctggtga
     4501 agtacgccgg ccggcagtcg caggcccggc tgatcgccga gaaggtggaa accgcgcagc
     4561 agttcgacat ggtgtcggcg ctgggcgtgc agctgttcca gggctatctg tttgcgcgcc
     4621 ccacggtggt gcagaccagg ctcctcagcc ccgggcaggc cagcatcgtg cagttgctga
     4681 acctgctgcg cggccaggcg agcaccgatg ccatcgagga agtcctgaag aaggacgccg
     4741 gcctggcctt caacctgatg cgcctgatca actccgccag cctggggctg acgcgcgaga
     4801 tcacctccat ccgccaggcg gtgctgctgc tgggcctgaa gaagctgttc cgctgggcgg
     4861 cgctgctgct gacggccgca cgcagcgccg gcacgccgcc ggcaacgggc cagacggccg
     4921 tggtgcgcgg ccgcctcatg gagctgctgg cgaacgaatt catgtcgccc gaggaggccg
     4981 accaggcctt cgtgatcggc gtcttctcca tgctggatca gatgctgggc atgccgcagg
     5041 ccagcgccgt cggcctgctg aacctgcccg atgaactatc gacggcgctg ctcgagcgca
     5101 gcaatatgca cggccggctg ctcaacctgg tcgaagcctg cgaaagcggc gacgacgaca
     5161 gcttcgacca ggccgcgcag gcgctgccgc tgaccagcca gcagatcaac tgggcccacc
     5221 tgcaggccct ggcctgggcc gatgccatca cccaggcctg accatctcat agccctttca
     5281 aagaattcaa tcaccaccca tgtccagccc caccgatccc atgaccgcag ccgagcccgg
     5341 cgctgaagac gccgacaacc aggtcgtcat cgcccgccag gccatcctgg atgaaaaccg
     5401 cgccgtcttc ggctatgagc tctttgaccg cagcacggcc acccacagcc ataccgcggc
     5461 cagcgatgcg gcgctgctgt tcaacgccct gtgctatgcc ggatccgagg cgctgggcag
     5521 caagaagatc gtcttcatca actgcacgca cgagagcctg gccggcggcc acctggagct
     5581 ggtccacccc gagaaggtgg tgctggaggt gccgacactg gccgccaacg ccacgccgga
     5641 gcagatagaa cagctgctgc ccacgctgca ggcgctgcgc acgcgcggct ttcgcctggc
     5701 cttcagccag gacgtgctgc gccgcgccta tggcagctgg ctgccgctgg cgaactttat
     5761 caagctggac atgctggcca tcaagcccga actgatcgaa ccgctggtga aatatgtgcg
     5821 cgccaacgcc caggccacgc tgatcgccga aaaggtcgag acggccgagc agcacgagcg
     5881 catggccgcc ctgggcgtga agctgttcca gggctactgg tttgcgcgcc cggccatggt
     5941 caaggcgcag accatcaggc cctcgcaggc caccatcatc cagctcatca acctggtgcg
     6001 ccagcaggcg agtacggacg aaatcgagga tctgctgaag aaagacccca cgctgtcgtt
     6061 caacctgctg cgcttcatca actcctcggg ctttgggctg tcgtgcgaga tcacctcgtt
     6121 ccgccacgcg gtgatgatcc tggggctgaa gaagctgttt cgctgggcgg cgctgctgct
     6181 caccacctcg cgcaaggatg gcacggcacc ggccgtaggc cagaccgcgg tggtgcgcgg
     6241 ccgcctcatg gagctgctgg cggccgagct gctgcccaag gaagaatgcg acaacgcctt
     6301 cgtggtcggc gtgttctcca tgctcgacag catgctgaac atgcccatgg agcgcgcgct
     6361 cgaaagcgtg gccctgcccg actcggtgca acaggccctg ctgcacaacc agggcgtgtt
     6421 cgcgcccttt ttggaactca ccaaggcctg cgaaagcggc gacgacgcgg cctttgcccg
     6481 ctgcgccgag gcgctgcacc tgtccaaccg ccaggtcaac tgggcccacc tgcaggccct
     6541 ggcctgggcc gaaagcctgg ctacagaggc ctgaagccgc agctgctacc cccgtggatg
     6601 gtgctgcgca tgcagctgct tgagccgctc gcgcgccaca tgggtgtaga tggtggtggt
     6661 ggagatatcg gcatggccca gcagcagctg caccacgcgc aggtcggcgc cgtggttcaa
     6721 cagatgggtg gcgaaggcgt ggcgcagcgt gtgcggcgac agcggcgcgg tgatgccggc
     6781 cagctgcgca catttcttca cgatgatcca gaacatcacg cgcgacatgg cgctgccgcg
     6841 ttgggtgacg aacagatcgt ccgtctgctg cccgcccagg atgacgccgc gcgcctcaag
     6901 caggtagcgt tccagccagc gccgcgcctc gtcgccaaac ggcaccagcc gctccttacc
     6961 gcccttgccc atgatgcgca gcacgccatc ggccaggccc aggtgaaagg tcttgagcgt
     7021 gaccagctcg gagacacgca ggccgctggc atacatcagc tccagcatgg cgcggtcgcg
     7081 caggcccagc ggcgtggacg gggcgggcgc atgcagcagc gcctccacct gcgcctgagt
     7141 cagcgtcccg ggcacgcgca gcggctggcg cgcagccagc aggcgcaccg tggggtcttg
     7201 cgtgatgcgc cgctcgcgca gcgcccagtg aaagtagcgc cgcagcaccg tcaggcgccg
     7261 gttggcggtg gtggcgcgcg tctgtgcgtg gcgcgcggca aagtagccct gcaggtggtg
     7321 ctcggccgtg gcgtccagca ccagcggcgg ctgttgctgc gccagccact gggcatacag
     7381 cgtcaggtcg cgccggtagg cagccagggt gttgcgcgcc aggccgtcct ccagccacag
     7441 ggcatctaga aacaagtcaa tggcggtctg actatccggc agcatgatga aaaacgcccg
     7501 tgggcggcgg gcggtgtgtt gaagcccgtg gcctattcca gcgtcagctt ctgcttttgc
     7561 accacttcct tgtacacggc attctcggcc ttgatctgct cggcaaactg ctcgggcgtg
     7621 ttggcgacga tgaaggaacc cgtgtcctcg atgcgcttct tcaccaccgg atcctccaac
     7681 accttcttca cgccggcatt gatcttgtcc accacctcct tgggcatgcc cttggggccg
     7741 acgatgccgt agaacgccag gcggttcacc ggctccagac ccacctcctt gaaggtgggc
     7801 acgttgggca gcacggccag gcgctggggc gcggcgacga cgatgggcac caggcggcca
     7861 tccttgatga agggcagcga ggacggcagg ttgtccaggt tcatgctgac ctggttggcc
     7921 accacgtcgt tcagggccgg gcccgcaccc cggtagggga tatgcgtcat gaaggtgccc
     7981 gacaggttct tgtacagctc catcagcaga tgctgaatgg tgccggtgcc cgacgaggcg
     8041 taggaatacc tgcccgggtt cttcttcagc tcggcaacaa agctctggta atccttggcc
     8101 ggaaacttcg ggttcacggc aatcacgttg ggcgtggccg ccacgttgat gacgggcgtg
     8161 aagtccgtca gcgcgttgta cggcaccttg gggttgatcg ccggggcggt ggccgtggtg
     8221 gacaccgtgg cgatgcccag gttgtagcca tcgggcgcgg cgcgcgcggt ctcctgcgca
     8281 ccgatgatgc cgccgccgcc gccgcggttg tccaccacca ccggctggcc caggaccttg
     8341 cccagcgggt cggcgatggc gcgggcaatg atgtcggtgg taccaccggg cgcgaacggc
     8401 accagcagct tgatgggctt ggcgggatag gcctgggcaa aggcggtgcc cgcggttgcg
     8461 agcagaacca gggccaggta gttacgacgt tgcatgcaaa cgctccttgt ccaatacgtc
     8521 caatgaaaga aacaaattgt aggccgcgat gctaccgacg cgcctggccg cgagcgccct
     8581 ggtaattacc ccggttgcac cgcgctatgc tgcgcgccgt gaactacgcg cagctgctca
     8641 tccccgactt ctcgctgatc ctgtgcggct atctggtctg ccgctacaca ccgctgaacc
     8701 gcacagtctg gcagcccgtg gaaagcctgg tgtactacct gctgtttccg gtgctgctgt
     8761 tccagtccat cgtcaaaagc cccatcgacg tgggcgaggc cacgggcctg gtcgtcgccg
     8821 gcgtgggcac cctgctgtgt ggcgtggccc tggcctacag cctgccgcac ctgccctggc
     8881 tgggccgaca tatcgaccgg cgcgaccatg cggcggcggc gcaggtctcg ttccgcttca
     8941 actcctacct ggtgctggcc ctggccgagc gcctggccgg cgcccagggt ctgctgatgg
     9001 tggcagtgct gattggcgtg tgcgtgccgc tggtgaacgt ggcggcggtg tggcccatgg
     9061 cgcgcggtgg ccagcgcggc ttctggggcg agctgctgcg caaccccttg atcatcgcca
     9121 cggccaccgg cctggtggcg aatgcgctgg gcctgcgcat ccccgcctgg ctggagcctg
     9181 cggtgagccg catcggcgcg gcgtcgctgg cgctggggct gatggccgcc ggcgccggca
     9241 tgcagttcgg cctgctcagg cgcggcaagc tgctcagcgc gtcggtgctt tccatccgcc
     9301 atgtggcgct gccgctgatc gcctgggcat gggtactgct gctggggctg gacgccactc
     9361 aggcccaggg gctgatggtg ttttgtgccg tgtccacggc gtccagctgc tatgtgctca
     9421 cggcgcgcat gggctacaac ggcgcctacg tggcggggct ggtcacgctg tccaccgccc
     9481 tgggcgtgct gagcctgccg ttcggactcg ggctattgcg ctgatactat tatttttata
     9541 gctgtttgcg ctttaccagc gggcgctacg gccgtttttc ttctaaaaga gcccaggcca
     9601 catgctcgcg caccagcgcg ctggggtcgt cggcacgggt gagcagcgcg gcgcgcagcc
     9661 gctcgtcgcc cgtggcgcgc agcgcattgc ccagcgccac ggcaatgttg cgcagccagc
     9721 gctcgtggcc aatgcggcgt atcgggccgc cctcggtcag gcgcaggaag gtggcctcgt
     9781 cccaggcgaa cagctgcacc agctgctggc ccaccagggg cgcgcgcgcg tcaaagtcgg
     9841 gcaggctgct ggcctgggcg tatttgttcc aggggcagac cagctggcag tcgtcgcagc
     9901 cgtagatgcg gttgcccatc agggggcgca gctcctcggg tatcggcccg gcatactcga
     9961 tcgtcaggta ggagatgcag cggcgcgcgt ccacgcggtg cggggcgacg atggcctgcg
    10021 tggggcacac gtccatgcag gcctggcagc tgccgcagtg ggcggtgacg ggctgcgtgg
    10081 gcgcaagcgc catgtccagg tagatctcgc ccaggaagaa catcgagccc gcctcgcggc
    10141 tcagcaccag cgtgtgcttg ccgcgccagc cctggccgct gcggcgcgcc agctcggcct
    10201 ccagcaccgg ggcggaatcg gtgaacacgc ggtggccgaa cgggcccacg gcgcctgcga
    10261 tgcgctcgct gagcttttgc aggcgcgcgc gcagcacctt gtggtagtcg cgcccccggg
    10321 cgtagacgga gacgatggcc tcccgcgggc gcgtcaggcg atcaaactcc acggcctgcc
    10381 agcccggcgg cgtggcgcgc ggcaaatagt ccatgcgcgc cgtgatcacg ctcaccgtgc
    10441 ccggcaccag ctcggccggg cgcgcgcgcc tgaggccgtg cgcggccatg taatgcatct
    10501 cgccgtggaa accttcggcc agccattgca tcaatccagg ctcggcggcg gacaagtcca
    10561 cgccggccac gccgatttgg gaaaatccga gctcgcgcgc ccagccccga atttgaggaa
    10621 cgagttgact gctgctgacc atcatcgccc gattgtagaa atgccgcccc cgctgtcgct
    10681 gcactggcag ggcgaggacg acgccgcccg ctttgccgcg cgcctggcgg cgcagcccgg
    10741 catcgccaac gccttcatcg ccctgcatgg cgatctgggc gcgggcaaga ccacgctggt
    10801 gcgccacctg ctgcgcgcgc tcggcgtggc cgggcgcatc aagagcccga cctatgccgt
    10861 ggtcgagccg caccaggcgc cgggcctggc catctggcat ttcgacttct accgcttcaa
    10921 cgatccacgc gaatgggagg acgccggctt tcgcgacctt ttcgccagcc cgggcctgaa
    10981 gcttgcagag tggcccaaca acgcatcagg gcttatccca gttgcggata ttgctattta
    11041 cattgaagcg gaggatgatc acgcccggca tgtcacgctg caggcccaca cgccccaagg
    11101 cagcgccgta ctgcaaggac tgaatacccc atgagcgacg agcgcccccc ccgaccgcac
    11161 cacagccgcc gccgcctgct gcaggcgggc agcctggtgc tgctgctggg cgcgcagcag
    11221 atcgcgcgcg gcgccagcat cgtggcagtg cgcgtgtggc ccgcggccga ctattcgcgc
    11281 gtgaccatag agtccgaccg cgccctcgtc gccaagcagt ttttcgtgtc cacgccgccg
    11341 cgcctggcgg tggatatcga aggcctggat ctcgatccat cgctgcgcga gctggtggcc
    11401 aaggtgcagc ccgacgaccc caacatcgcc ggcatacgcg tgggccagaa cacccccggc
    11461 gtggtgcgcc tggtggtgga cctgaaacag gccgccaggc cgcaggtgtt cacgctcgcg
    11521 ccggtggccg cctaccagca ccggctggtg ttcgacctct accccgccnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gcgcagcacc gaccggctga tcatcgtcgc
    11701 gctcgacccc ggccatggcg gcgaagaccc gggagccata ggccccggcg gcacgcgcga
    11761 gaaagacgtg gtgctgaagg tcgcgcgcca gttgcgcgac cgcatcaacg ccacccaggt
    11821 gggcggcaac ccgatgcgcg cctttctcac gcgcgacggc gattttttcg tacccctggg
    11881 cacgcgcgta gagaaggccc ggcgcgtgca ggccgacctg tttgtctcca tccatgccga
    11941 cgccttcacc actccgtccg cgcgcggcgc cagcgtattc gccctgagcc agggcggggc
    12001 atcgagctcg gccgcgcgct ggctggccag caaggagaac gaggccgacc tgataggcgg
    12061 cgtgaacgtg ggcagccagg accgccatgt gcagcgcgcc ctgctggaca tgagcaccac
    12121 ggcgcagatc aacgacagcc tgaagctcgg cagcgtgctg ctgggcgaga ttggcggcat
    12181 ggcccggctg cacaagccgc gcgtcgagca ggccggtttt gcggtgctga aggcacccga
    12241 cattcccagc gtgctggtcg agaccgcctt catcagcaac cccgaggaag aagccaagct
    12301 gcgctcggcc gcctaccagg agcagctggc cgacgcgctg atgcgcggca tcgtgcgcta
    12361 cttcgccaag aacccgccgc tggcacgcag ccgcaccgtg tagcggcgcc aggcccgccg
    12421 ccatacggcg cgcaaggcgc tgcgcgccca ccagggttaa cccaggcgtt ataaaaccca
    12481 tcgcatatcg agtatcaggg ggcctgaatg cgcaagtgcc ccgcgtgggt gcaccattgg
    12541 cgcaacgacg gcattgccct gcccgcttgc cctttggact gcccatgaca tacgccacac
    12601 cctcagacca cagccgcgcc atccgcttct atcaccgggg tgagatcgtc agcgtgagcg
    12661 gcgtgcagac cacgcgctcg gtgctcgact ggctgcgcga ggatgcacgc cacaccggca
    12721 ccaaggaagg ctgcaacgag ggcgactgcg gcgcctgcac cgtggtcgtg ggcgagctgg
    12781 acgccgctgg ccagctgcag ctggccagcg tcaacgcctg catccagttt ctgcccacgc
    12841 tcgacggcaa ggcgctgttc accgtggaag acctgagcca gctggcaccg gcgggcgcca
    12901 gctgctgcgg cggcgcttgc ggcagcggca cccagcccca gctgcacccg gtgcagcagg
    12961 cgctggtgga ctgccacggc tcgcaatgcg gtttttgcac gccgggcatc gccatgacgc
    13021 tgtggaacag ctacgaggcg catcaaagcg ccggcacacg cccgacgcgc cagcagctgg
    13081 ccgacgcgct gtctggcaac ctgtgccggt gtaccggcta ccgccccatc ctggacgcgg
    13141 gcgagcgcat gttcgacctg cccgatgcgc gccttgaccg cgccgccgtg acggccgccc
    13201 tgcaagagct ggccaaggcg ccggcgcttg cctacagcgc gcccaacccg gccgctagcc
    13261 cggcgcgcag cgaccgcttc cacgccccgc gcacgctgga cgacctggcc gcgctgcgcc
    13321 tggcgctgcc ccaggcgcgg ctgctggccg gttcgaccga catcggcctg tgggtcaaca
    13381 aacagtttcg cgatgtcggc gagctgatct atgtgggcca ggtggccgag ctgcgcaccg
    13441 tcgaggaacg cggcgaccag ctgtggatag gcgccggcgc ctcgctcgaa gatgcctggc
    13501 gcgccctggc cgcgcgcgcg ccaacgctga ctgactgctg gctgcgcttc gcctccacgc
    13561 cgatccgcca cgccggcacc atgggcggca acgtcgccaa cggctcgccg attggcgacg
    13621 caccaccgat tctgatgacg ctggacgcgg ccgtggagct gcgccgcggc gccgccgtgc
    13681 gccgcctggc gctgaccgat ttctacctgg actacatgaa aaacgcgctg caagagggcg
    13741 agttcgtgca ggccatcacc gtgccgctgg cggcctttgc gcgccagatt cgcggctaca
    13801 agatcagcaa acgttttgac tgcgacatct cggcgctgtg cgccggcatg gccatggagc
    13861 tggacggcga caccgtggcc agcgtgcgcc tggcctacgg cggcatggcc gccacggtgc
    13921 gccgcgccgc ccaggccgaa gccgcgctgc tgggccagcg ctgggacgag gccgcggtgc
    13981 gcgccgccca ggccgcgctg gcgcgcgact tcgcccccct gagcgacatg cgcgccagca
    14041 gcgcctaccg cctgcaggtg gcgcaaaacc tgatccagcg cctgtggctg gagacgcgcg
    14101 ccaccaaccc gctgggcagc gacgccacca gcgtgttcag cgtcatgcca cacgccgcag
    14161 cgtaaggaga aacgccatga accagcccat ctccagccag ctgctggccc gcccggtggc
    14221 ctttgccccc tatcaagaca accacggcct gcgcctggac cccgccgccg aggcccgcgc
    14281 ccacgccgcc ggcgcccgcg tgggcatcag ccaggcgcat gaatcggcgc agctgcacgt
    14341 caccggcagc gccacctata ccgacgacat tcccgagctg gccggcaccc tgcactgcgc
    14401 gctgggtctg gcgccggtgg ccgccgggcg cctgctgtcc atcgacctgg cgctgctcgc
    14461 cgcccagccc ggcgtggtgc gggtgttcac cgcggccgac attcccggca gcaacgactg
    14521 gggctcgatc gtgcacgacg accccatcct gtgcgacggc gacatccgct acctgggcca
    14581 gccggtgttt gccgtggtgg ccgagtcgcg cgagcaggcg cgccgcgccg ccgccctggc
    14641 caaaaagctg attcaggccg aggccgccac gccggtgctg acgccgcagg acgcccacgc
    14701 caagggccag tacgtggtgc cgcccatgca tctggtgcgc agcagcagcg gcctggatga
    14761 ggccggcata caagcgcgca tccaggccgc gccgcaccgc ctgcagggcc aactggacgt
    14821 gggcgggcaa gagcagttct atctggaagg ccagatcagt tacgccattc ccaaagaggc
    14881 cggcggcatg catgtgcact gctcgaccca gcaccccagc gagatgcagc acctggtggc
    14941 gcacgccctg ggcgtggcgg cgcacgccgt gctggtcgaa tgccggcgca tgggcggcgg
    15001 ctttggcggc aaggagtcgc agtcggcgct gttcgcctgc atggccagcg tggccgcgca
    15061 gcagctgcag cgcccggtca agctgcgcct ggaccgcgac gacgatttca tgatcaccgg
    15121 ccggcgccac tgcttctggt acgagtacga cgtgggctat gacgacgccg ggcgcatcct
    15181 gggcgcggcc gtcaccatgg tgtcgcgcgc cgggcattcg gccgacctgt cggcgccggt
    15241 gatgacgcgc gcgctgtgcc acttcgacaa tgcctactgg ctgcccgagg tcgccatgca
    15301 cggcttttgc ggcaagacca acacgcagag caataccgcg tttcgcggct ttggcgggcc
    15361 ccagggcgcg attgccatcg agtacatcct cgacagcatc gcacgccagc tgcagcaaga
    15421 cccgctggcc gtgcgccgcg ccaactttta cggcaccgcc acgcacaaca tcacccccta
    15481 tctgcagccg gtgcgcgaca acgtcatcca cgaactggtc gaccagctgg aagagcgcag
    15541 cagctaccag gcacgccgcg ccgccattgc cgcattcaac gccaccagcc cggtgctcaa
    15601 gcgcggcctg gcgctgacgc cggtgaagtt cggcatcagc ttcaacgtca agcatttcaa
    15661 ccaggccggc gcgctggtcc atgtgtatac cgacggctcc atcctggtca accacggcgg
    15721 caccgaaatg ggccagggcc tgaacaccaa ggtggcgcag gtcgtggcgc acgagctggg
    15781 cgtggacttt gcccgcgtgc gcgccaccgc caccgacacc agcaaggtgg ccaacacctc
    15841 ggccacggcg gcgtccaccg gcaccgattt gaacggcaag gcggcgcagg acgcggcgcg
    15901 ccagatccgc gagcgcctgg ccgcctgcgc cgccgcccgc cacggcggcg cggccgagga
    15961 cgtgcgcttt gccaacggcc aggtcacggt ggccggcaag acgatcagtt ttgaaaccct
    16021 ggtcggcgag gcctacctgg accgcgtgca gctgtggtcc gacggcttct acgccacgcc
    16081 cggcctctcc tgggaccgcg aaaaaatgca gggcaacccg ttctactact tcgcctacgg
    16141 cgccgccgtc agcgaggtgg tggtggacac actgaccggc gaatggaagc tcttgcgcgc
    16201 cgacgtgctg cacgacgtgg ggcgctcgct caacccggcc gtggacatag gccaggtcga
    16261 gggcgccttc atccagggca tgggctggct gaccacggaa gagctggtct ggcatccgca
    16321 aaccggcctc ttgatgacgc acgcgcccag cacctacaag attcccacgg ccaacgactg
    16381 cccgccggag tttcatgtgc agctgttcga gggcgccaat gcgatggact cgatccaccg
    16441 cagcaaggcc gtgggcgagc cgccgctgtt gctgccgttc tcggtgttct ttgccatccg
    16501 cgacgcggtg gccgctgccg gcgggcacca ggctctgcca ccgctgcagg ctccagccac
    16561 cagcgaggcg atcctgcgcg ccattggcgc cgtgcaggcc ggctgaaatc ccacgccatg
    16621 ccgccctcaa caccccccac cgcgcccgcc ggtgccggcg atcagcacct gttcgaccgc
    16681 atagacctgc acctgatacg cgtgctgcac accgtgctga ccgagcgcag cgtctcgcgc
    16741 gcggcgctgc gtctgggcat gcaccagccg gcggtatcca cggcgctcaa gcgcctgcgc
    16801 gagctctcgg gcgatccgct gctggtcagg aacggcgccc acatggtgcc caccgaggcc
    16861 ggccagcgca tgctggagcc cgccgccaac gtgctgcgcg cggccgagac cctgttcacc
    16921 gaggcgcgca gctttgaccc gcgcaccagc tgccagacct tccacatcgc cgccagcgac
    16981 tacctcgatc cgctgttcct gccgcgcctg gtggccgaca tcaagcgcca ggcaccgctg
    17041 gccagggtgg agatccatgc gctgacgcgc gaggccgact accgtgccca gctggccctg
    17101 ggcgagatcg acctggtcat aggcaactgg ctgcagcccc cggacgagct gcacctggca
    17161 cggctgttcg aggacgaggt ggtgtccctg gtcagccgcc accacccggc cgtgcgccgc
    17221 ggctgggacc aggacgcctg gctggccgcc gagcatatcg cccccatggc cacgcacccg
    17281 ggcgcgcgcg gcatcatcga ccagatgctc gacggcctgg ggctcaagcg ccatatcgcc
    17341 gtgcgcagcg cctacttcag cctgataccg gacatggtcg ccggcaccca gctggtgctg
    17401 accacggggc gccgctattg cgaacgcgtg gccagccgcc tgcccctggt gatcctgccc
    17461 tgccccgtgg ccctggcgcc actgcagtac taccagctgt ggcatgcgcg cagccagcat
    17521 tccagcgccg cacgctggct gcgcgagcgc acgcgcgacg ccattgccgg cctgcagctg
    17581 cagcccacaa cgccgctctc accgctttcc tgagcacaac accacaagga gacatacgcc
    17641 atgaatgcca aggacctgac aggcatgccg accacgccac cacaagccgc ggccacgggc
    17701 ttgttggagc ggctgttcaa gctgcgcgag catggcacca cgccgcgcac cgaggtgatc
    17761 gccggcatca ccacctttct gacgatggcc tacatcatct tcgtcaaccc ctccatcctg
    17821 ggcgatgccg gcatgcccaa ggacgcggtg ttcgtcgcta cctgcctgat agcggccttg
    17881 ggctcggcca tcatggggct gtacgccaac taccccatcg ccatggcgcc gggcatgggg
    17941 ctgaacgcct actttgccta cgccgtggtg ctgcacatgg gctttacctg gcaggcggcg
    18001 ctgggggcgg tgttcatctc cggctgtttg ttcctggtcg tgacgctgtt cggcatacgc
    18061 aggcacatca tcgacggcat accgcagtcg ctgcgcagcg ccatcaccgt gggcatcggt
    18121 ctgttcctgg cactgatcgc gctcaagagc gccggtatcg tcgtcggcga caaggccacg
    18181 ctggtgacgc tgggcgacct gcactcggcc cctgtgatcc tggcggtgct gggttttctg
    18241 gccatcgtgg cgctggacaa ggcgcgcgtg ccgggcgcca tcctgatcgg catcgtcggc
    18301 gtcaccgtgg cctcgttctt ctttggcggc aaccagttcc gtggcatctt ttcggcgccg
    18361 ccatcgatcg cccccacctt cctgcaactg gacatccaca ccgccgtcac caagggcctg
    18421 ctcaacgtgg tgctggtgtt cttcctggtc gagctgttcg atgccaccgg cacgctgatg
    18481 ggcgtggcca agcgcgccgg cctgctggtg ccgggcaaga tggagcgcct gaacaagtcg
    18541 ctgctggccg acagcggcgc catctttgcc ggctcgctgc tgggcacgtc cagcaccacc
    18601 gcctatatcg agagcgcctc cggcgtgcag gccggtggcc gcaccggcat gacggcgctg
    18661 accgtggccg tgctgttcct ggcctgtttg ttcatcgccc cgctggccgg cgtggtgccg
    18721 gcttacgcca cggcgccggc gctgttcttt gtcgcctgcc tgatgctgcg cgagctgatc
    18781 gacctgaact gggacgacac caccgagacc gtgcccgccg ccgtcaccgc gctggccatg
    18841 ccgttcacct actccatcgc caacggcctg gccttcggct tcatcaccta cgccgcggcc
    18901 aagctgttca ctgggcgcgt gcgcgaggtg cacttcatgg tgtggatcat cgccgccgtg
    18961 ttcctgttca agttcttcta catcggcgga cattgacacc atgagccccc acgcctttcg
    19021 cgcgcagttg ctgcgctttg acgccgacgg ccagccgctg tatgacgagg acggcctgct
    19081 ggtcactgcc accgacgccc agggcgtgcg gcgcgtgctc gatgccggcc cctttggcgc
    19141 gctgcgcgag cgccatgccc aggttccgct cacgcactgg cccgggcgcc tcatcgcccc
    19201 cggcttcatc gacatgcatg tgcactaccc gcagctggat gtcatcggcg cgcccgccga
    19261 cggcctgctg ccctggctgg agcactacac cttcccggcc gagtcgcgct ttgccgaccc
    19321 ggagcatgcg gccgaggtgg ctggcttttt ctttgacgaa ctgcagcgca atggcgtgac
    19381 cacggcgctg acatttgcca cctcgcaccc ggcctcggtg gacgctttct tcggccaggc
    19441 acagcggcgc ggcctgcgca tgatcggcgg caaggtgctg caggacaggc acagccccga
    19501 cggcgtacgc gatgccaccg agcaaagcct gatcgacacc gaggcgctga ttcagcgctg
    19561 gcatggccag ggccgtctgg gctacgccat cacgccacgc tttgcgccgt cgtgctccga
    19621 cgcgcagatg cacggtgcgg ccgagctggc gcgccgtcac cccgacacct ggatacagtc
    19681 gcatgtggcc gagaaccggg acgaggtgcg ctgggtgcgc gagctctacc cgcaggcgcg
    19741 ctcctacctg gacgtgtacg ccggcttcgg gctgttgcgc cggcgcgcgg tgtacgcgca
    19801 ttgcatttat ctggacgaca ctgaccgcca gctgatgcac gaaacgggca ccgccgccgc
    19861 cgtcagccca acgagcaacc tgttcctggg cagcggcttc ttcaacttca tcggcgccga
    19921 tcgactgggc atgctgcacg gcctggccag cgacgtgggc gggggcacca gcttcagccc
    19981 gtttcacacc atgctggcgg cctactacgt gggccgcgaa ggccagacca agccgggctt
    20041 gagcctgaaa ccttcgcagc tatggtggca gcacaccggc ggcgcggcgc gcgcgctggg
    20101 gctggagggc gtgataggga cgctccaggc cggcaccgag gccgacttcg tggtactcaa
    20161 cccccaggcc acgccgctgc tggcgcgcaa gacggcactg gcgggcagcc tggaagagct
    20221 gctgttcgcc ctgatcgtgc tgggcgacga ccggctggtc gagcgggtag tgatcgctta
    20281 agccggctgt tgacgccccg cgcgccaggc accaaagatg gcgatcagcc ccggcaccag
    20341 gatcaggccc cagatgatct tctccagatg ctggcgcacc cagggcaggt tgccgaagaa
    20401 gtaaccggcc gtggcaatgc ccagcaccca gatcagcgcc ccggccacgt tgtaggccgt
    20461 gaacttggcc cgccccatgg ccgccacccc ggccacgaag ggcgcaaagg tgcggatgaa
    20521 cggcatgaag cgcgccagca cgatggtgat gccgccatag cgctcgtaaa acgcatgggc
    20581 ctggtcgaag gccttcttgt tgaaccagcg cgaaccctcc cactggaaca ccttggggcc
    20641 aagataacgc ccgatgctgt aattgcattg atcgcccagg atcgccgcaa cgacgagcag
    20701 cgtgcaggcc agcggaaagt tgagcagccc cgcaccactc agggcaccga cgatgaacag
    20761 cagcgaatcg ccgggcagga agggcatgac caccacgccc gtctcgacga acacgatgag
    20821 aaacagcagc gcatacaccc aggcgccgta actggcgacg aaggcctcga gatgcttgtc
    20881 cacatgcagg atgaaatcga tcagaaacgc aatgatgtcc acgtagcaag cccttcgaat
    20941 tgacggcgcg taatgtaccc gggcgcgcca tgtccgtacg gtgtgcgaca caaacatggc
    21001 aagtggacac agttgctcgc aatcggcaac cagatagcca tacacacttc acaccctgcc
    21061 gccacagtgg cagcacatca tttgttccac ccattccgtt tccaaatcat tcgtgtctag
    21121 gcgcgaagcc gcaggcagtg ctgtagcacg acaaggcgaa gcaacaacga cacgggtgat
    21181 ttagaaatgg aatcatccac caaggaatgc gtttcatgat cttccgtcgt acccacaccg
    21241 tccgcgccct gaccgtcgcc accgcactgg tgggcgccct ggcgctggcc ggctgcgccc
    21301 accaccccag caatgcccag atcggcaccg gggtgggcgc cgtcgccggt ggcctggtcg
    21361 gcaatgcggt gtttggctct accctgggca ccgtgggcgg cgccgccgca ggcgcgctga
    21421 ttggcaacga agtcggcaag aacaacgacc gcggccgtgg ccgccgcgga catcgccact
    21481 gatcgccctg gcacccggcg ggccagaccg gccccctaca atcgccgcgt gaccgcccag
    21541 ccctccgacc ctgccacccc gtccccccgc cgcccgatac gcgacctgcc cgatgagctg
    21601 atcagccaga ttgccgcagg cgaggtggtg gagcgcccgg cctccgccgt gcgcgagctg
    21661 gtggacaacg cgctggacgc cggcgcgcgc cagatcacgg tacgcctggc cgcgggcggc
    21721 gtgcgcctga tcgccgtcga ggacgatggc tgcggcattc cgcgggatga gctgccggtg
    21781 gccctgcgcc gccacgccac cagcaagatc accaatctgc acgacctgga atcggtcgcc
    21841 accatgggct ttcgtggcga ggccctggcg gccattgcct cggtgtccga gatggcgctg
    21901 ctgtcgcgca ccgcgcagca ggccagcgcc ttcgcactcg acgcgcgcag cggcgagcta
    21961 cgccccgcgg cgcgcagcac cggcaccacg gtggaggtca aggagctgtt cttctccacc
    22021 ccggcgcggc gcaagttcct gaaaaccgac gccaccgagc tggcgcattg cgtggaggcc
    22081 gtgcgccgcc acgcgctggc gcggcccgac gtgggctttg ccatctggca cgagggccgg
    22141 ctggtcgagc agtggcgcac caccctcgcc cccggcgtgg acgccgacgc cgccctgcag
    22201 cagcgcctgg gcgacgtgct gggcgacgac ttcttgcgca actccgtgcc cgtgcaactg
    22261 cgcctgggcg ccgtcaccgt gaccggccgt gccggcctgc cggacgccgc gcgctcgcgc
    22321 gccgaccagc agttctgcta tgtcaacggc cgcttcgtgc gcgacaaggt gctcacgcac
    22381 gccgcgcgca gcgcctacga ggatgtgctg cacggccaga agcagccggt gtatgcgctc
    22441 tacgtgcaga tcgacccgca gcgggtggac gtgaatgtgc acccaacaaa aatagaagtg
    22501 cgctttcgcg aaagccgcga ggtgcaccag gccgtgcgcc acgccgtgga gaacgccctg
    22561 gccgccccgc gcgcccaggc attggccgct gccagtgcga cgcccgctgt ggcaccccca
    22621 ggtgattttg aacaaaatag gcctgtagcg cccaactggc aagcgcaaac agctattaaa
    22681 tttgatgagc gcatgggcca taaggtctcc gacctgcagg cgctatgggg cacgccggcg
    22741 gcgcagcctg cgccccccgg gcccaccatg gagcccgccg gcgcaacctc gcctgctgcc
    22801 gccatgcccc tgccagctgc ggtcgaaacc cctgccgacg cagccaacac ctggcccctg
    22861 ggtcgcgccc tggcgcagct gcacggcatc tacatcctgg ccgaaagcgc ccagggcctg
    22921 gtgctggtgg acatgcacgc agcgcatgag cgcatcgtct acgagcgcct caagtcccag
    22981 gtcgatcagg gcgcgcgcat cgccagccag ccactgctca tccccgctac cttcgccgcc
    23041 acccccgagg aggtggccac ggccgaggcc catgccgaca cgctgggcct gctgggcctg
    23101 gaggtggtac cgttttcacc gcgcaccctg gccgtgcgcg ccgtgcccac ccagctggcc
    23161 cagggcaacg ccgcggagct ggcgcgcagc gtgctcgccg agctgggcca gcatgacgcc
    23221 agcaccgtga tccagcgtgc acgcaatgag atcctggcca ccatggcctg ccacggcgcc
    23281 gtgcgcgcca accgccgcct gacgctggac gagatgaacg ccctgctgcg ccagatggag
    23341 gtcaccgagc gttccgacca gtgcaaccac ggccgcccca cctggcgcca gctgaccatg
    23401 aaagagctgg acgggctgtt tttgcgcggg cgctgacgca agagcccgcg caagcgggcc
    23461 gcaggcttca gtcgcgcgcc gccttggcgt ggtcggggaa gtcggcaaac accccatcca
    23521 cgcccagctg gtagaacagg cggaactcgg ccttggggtc acccttgaag tccgatgcca
    23581 ggcgccgcgc ctcgctgcgg aaggtgtagg ggtggacgaa cagcccggcg gcatgggcgt
    23641 tcttgaccac atcggtggcc ggcagcatga cccggtcgcg ttcgtcgatc ttgccgtcac
    23701 cgttgaggtc atcaggcttg ccgtccttgt tggcgtcgac cagcttggat gggatcagat
    23761 agggcttcca cgggccgatg ccgtcggcgt aactcttgac gtccttgagc ccggccggcg
    23821 tcagcaggct ggcaaaggtg cgggcgtcgc cagccacggc aaagtcgtac ggcttgtcat
    23881 agggggctgt caggtccatg ctgccgtcgg cgttgacgtc gttggcatcc accagctgca
    23941 ccaggcgcac ctgggtcttg ctgcgcagat acttgaggtt ggccacctcg aaggactgca
    24001 cgatcaccgg cgcgctcttg ctggtgtaac cgtacttgcc caggatggcg agcaagcggt
    24061 cttccagcgc cagacccagg cccgcgtggt agctcggatg cttggtctcg ggatagacgc
    24121 cgatgctgcg attcaggcgt gcaccttcgg tcttggccag ctccagcacc tcttcgaagg
    24181 tcgggatcag atacttgccg ttgtggctct ggtcgcgttc ggccaggggc tgcacggcgc
    24241 gcagggtctt gagctcggcc agggtgaaat cggaaacaaa ccagccctcc tcgctcaccc
    24301 catcgaccag cttcttgctc ttgcgtgcgg caaattcgct gcgctgggcg acatcggtgg
    24361 tggcggtgat attgggttcg tggcgcgcca ccaacacccc gtccttggtg gcgacgagat
    24421 caggctcaat gaaatcagcg cccagctcta tggccttgcg gtagccctcc agggtgtggt
    24481 ctggcaggta gcccgaggcg ccccggtgac caatcaccag gggcttgtca cccgtcaggg
    24541 ttggataacc acccttatcg tcaccaccgc cacaggccgc cagcaacacc gctgccgctg
    24601 ccagggccac ggccccttcg aaacattgtg aatacgcatc gcttgcacct ctcttgaaaa
    24661 atcagactat gggccgcgat cgtgacaaaa aaaagacgct gcaggcatgg cgaatcccca
    24721 ccccaccccg tgccacgcta cccaagcaca gcattgcgcg tgagcgccac actaactttt
    24781 gcacggctta tgcatgcacg catgctttat tggcataatt ccatgctgca acgccgcaat
    24841 ttttctctcc gattcgaagg tttcccaatg aagcacgcat ccgtaggcca attcctggaa
    24901 caactggccc agcgcaaccc cggtcagccc gagttcttgc aagccgtgac cgaggtcatg
    24961 gaaagcctgt ggcccttcat cgagaagcac ccgcgctacg ccgaacaggc actgctggag
    25021 cgcctggtgg agcccgagcg ggtgatcatg ttccgcgtct cctggttcga tgaccatggc
    25081 aacgtacatg tcaaccgcgg ctaccgcatc cagcacagca tggccgtggg gccgttcaag
    25141 ggcgggattc gctttcaccc ctcggtcacg ctgtcggtgc tgaagttcct ggccttcgag
    25201 caaactttca agaacgccct gaccaccctg cccatgggcg gcggcaaggg cggctctgat
    25261 ttcgacccca agggcaagag cccgaccgag atcatgcgct tctgccaggc cttcgtgacc
    25321 gagctgttcc gccatgtggg cccggacacc gacgtgccgg cgggggacat cggcgtgggc
    25381 ggccgtgagg tcggcttcat ggccggcatg tacaagaagc tggccaacaa cgccgccagc
    25441 gtgttcaccg gcaagggcct gtcgtttggc ggctcgctga tacgtcccga ggcgacgggc
    25501 tatggctgcg tgtactttgc ccaggaaatg ctggccacgc gtggacaaag cctggaaggc
    25561 aagacggtgt ccgtctccgg ctccggcaac gtggcccagt actcggtcga gaaggccatg
    25621 caactgggcg ccaaggtggt cacggtgtcc gactcttcgg gcacgattta cgaccctgaa
    25681 ggcttcaccg ccgagaagct ggccatcctc atggatgtga agaaccacca ctacggccgc
    25741 gtgaatgact acgccaagcg caccggcgtg gaattcctgc ccggcaagac gccctggcat
    25801 atcccagctg acgtggccct gcccagcgcc acgcagaacg aactcgacga gcaggacgcc
    25861 cgcacgctga tcaaaaacgg cgtgctgtgc gtggcggagg gcgccaacat gccttcgacc
    25921 aacgaggctg ccaaggtgtt cgaaacggcc ggtgtgctct acgcgcccgg caaggccagc
    25981 aatgccggcg gcgtggccac ctcgggcctg gagatgagcc agaacgcgca gcgcctgtcc
    26041 tggacacgcg acgaagtcga tgcccgcctg ctgcagatca tgaagagcat ccatggcgcc
    26101 tgcctgcagc atggcaagcg tgccgacggc agcgtcagct atatcgacgg cgccaacatc
    26161 gccggcttcg tgaaggtggc cgacgccatg ctggcccagg gcgtggtctg atctacagcg
    26221 ctatcctggc gcaccagggc aggtctccca gtgcgtcgta gtgcgcgacg agtaccgcac
    26281 gccctggcca gcgcggcgcg cgggcctcag cctccagggc ctgcagcaga tagctgatcg
    26341 atggtttatc caacgctgcc aacggctcgt ccagcaaggt cagcacggcg ccgctggcca
    26401 gggctgcagc cagcagcacc ttgcgctggc tgccgctgga gagctgatac atgggcttgt
    26461 gcaggtgcat tgccaggcca aaaccggcga catgtgcgcg ccaggcttcg cctgaccagc
    26521 gcggatggtg cgcaggcagc tgctccaccc atgtcagcgg tgtcaatgtg tccggccagg
    26581 gcgcacgcgg atcgcgccag aacacctggg cgcgatattc cgccggttgc cgcacggcat
    26641 ccatcccgag caatgtcaca ctccccgcct gcggcgcagt ttcgccggca aacaggcgca
    26701 acaggctggt tttgcccgtt ccatcgcccc cttgtatcag ggatagcccg gctggaaaat
    26761 ctgcactcca atcctggaac aggctgtgtc cagggaaccc gaaccccagg ccctgcacat
    26821 gcagcacggg tgcgttgctg gtgggttgcg acgacatggt cagcaccttt cgttttatcg
    26881 tctacgggac ggcatctgcc gcacgtcatg aagctgcatt ttctcgattt tgactacagc
    26941 gaagacgcag aaggcacggc cacctgggac gccatcgctg ccatacccgc agcgcgcatg
    27001 agtgagctgg agcacgagat catcagcgtg ctggcctggg cgcatgcgga atatggtgag
    27061 ctgcgcggcc ccctggaatc cggtggcctt tgggattacg acctgcagtg cgagcgtgat
    27121 ggccaccctc tgctggcgat gagctataac accgaaaccc ggcgactgga gccattgccg
    27181 cagttgccgc ctgatgcacg cgtggccctg actctgtcac tatgcggcgg ccaggcgttt
    27241 gacgaggcct ttgcggcgcg tttcggcgcc gcatagtcca aaccagcgcc ccttgcctgc
    27301 ttattgcaga gattctgtag ccgcgtctgg cagcagcaat ggcagcacgg caatcccctc
    27361 atccagcaac tctgccgttt ctcctggaga ggcctggccc cgaatggggc gctcctcgat
    27421 ctcaccgtaa tgcatgcgcc gggcctcgac agcaaagcgt tgcccgacat cctcggtgcg
    27481 cgccacaatc tgccgggcca tatgcagcca ggccgcgtgc atcgcctggg gtgtcacggc
    27541 cccgttggtg gcttgcactg gctgagttgg ctgggacgcc tgagatccgg catcagaaga
    27601 cgagcgcaga ttcaggcgtg gcgcgcttaa ccccttgcga atatgggcat tgccacacag
    27661 ggggcattgc accagaccac gcgccaactg actctgaaag tcgtcctcgc taccaaacca
    27721 gccctcgaag gcatggtcat cggcgcaaaa caaatccaga accttcatga cacacccgtt
    27781 cagtgtgcag ggcgcctgcc acgatgcatc agatagcggt agacgaatcc gggaaatgcc
    27841 aaggtgatga acaaggcacc ggtcacggca tagaactccc agccttgagg cgcaatctgt
    27901 ccggcgcgct tttcgatcag cagcgccagc gcacccacca gaaagtacag cagcagcagt
    27961 tcgagcgcac gcgcaaagaa tggtttgccc tggtgtgccc gcgcgccgac caccaaccaa
    28021 cggtcattga taaaaggtag attggccgcc acaagggcgg ccaagatcac cagccaaatg
    28081 gctgcagtct gggtcatggg ttgaacaata cgcccatcag gtcgccaatg cgcgcacgat
    28141 ggcgtcagca cacaaggcca tcaagccacc tggaagaacg cccagaatca gcaccaaggc
    28201 gccattgatg gaaagaacca cgcgcgcatc gaagggcgcc gacaccttgc cagccgtgag
    28261 cggcgcatcg aaatacatga ccttgactac gcgcaggtag tagaaggcgc caatcagcga
    28321 catgacgacg gcaaacaccg ccagggccac atgcaggcca tggccagacg ctaccagcgc
    28381 ctgcagcacc gacagcttgg cgtaaaaacc caccagtggt ggaatgcctg ccagcgagaa
    28441 catgcagacc gccatcacgg ctgcgtacag cgggctgcgc tggttcaggc cagccaggtc
    28501 ggcgatttcc tcactctcga aaccttcgcg tgcgagcatg gcaatcaggc caaaggcagg
    28561 cagcacggtc agcacatagg tgacgatgta gaacatggcc gagctgtagg cggtttcggc
    28621 agcattggca tccaccatgc cgttgaccac tcccgacaac aggcccagaa acaggaaacc
    28681 catgtgcgaa atggtcgaat aggccagcat gcgcttcaga ttagtctgca acacaccggc
    28741 cacgttgccc acgagcaatg aggcgatggc cagcactgcc agcatctgtt gccagtcaat
    28801 cgccagcggc agcaggccat cgaccaggag gcgaatcgcc atggcaaagg ccgcaaactt
    28861 gggtgcgcta ccgatgatca gcgtgaccac cgtgggtgca ccctgataga cgtcaggaat
    28921 ccacatgtgg aacggcacag caccgaattt gaaggccagg ccggcgacga tgaagaccac
    28981 gccaaacacc agtacctggt gacgaatctg gcccgcattg atggccttga acacctggcc
    29041 gatgtccagg gaaccggtgg cgccatagac catggacagg ccatagagca gaaagccact
    29101 ggccatggca ccgagcacga agtacttcat cgcggcctcg gtggccgtgg tgtgatcacg
    29161 gcgcagcgca accagcgcat agctggacag cgtgagcaat tccaggccca gatagatcac
    29221 gatgaagtta ttggccgaaa tcagcacgca aatgcccagc agcgcaagca tggtcagcgt
    29281 gaaaagctcg cctccacgca acatgccgcg gtcaagcgca taggaacggc cataaaccag
    29341 cgtcaccatc agtgacactg tggcaaagca cttgagccag ttacccatgg catcgctgac
    29401 caccatgttg ccccagccat agaaggtgtt gccactgctg gcatacatgg cctgcatcac
    29461 ggcaaccacg gccagagaaa gcatggtcag cacataggtc gccgtgcgtc gcgtacttcc
    29521 cacgcccaga tctaccagcg caatcacgca ggccatgacc agcagcacga tctcagggta
    29581 aatcgccaac cagctgatgt tgtcaatcat cttgtatctc tcgcttcgct agtccggtca
    29641 gttcagcttg gtttgcgcca catgcttgag cagctcggcc accgacacat ccatcacatc
    29701 ggtaaagggc ttgggataaa tgcccatgta gagcacggcg atcgccagca cgcccaacac
    29761 caggaattca cgcccattca ggtcggtcat ggcacggacg ttgtcattgg tcacggggcc
    29821 cagatagacc cgtttgtaca tccacagcga gtagctggca cccaggatca cggcaatcgc
    29881 agcacccagg ccaatccaga aattgaaacg caccgaggca atgatcacca tccactcgcc
    29941 aacgaaacca gccgttgcag gcagaccgca attggccatg gagaacagca gcgcaaacgc
    30001 cgcaaacttg ggcatggtgt tgaccacgcc gccgtagtct gcaatctggc gggaatgcac
    30061 gcggtcatac aacacgccaa tgcacaggaa catggcgccc gacacgaagc cgtgggcaat
    30121 catctgcgcc agccctccag acacgccaag gtcgttgaaa atgaagaagc ccagcgtgac
    30181 aaagcccatg tgtgccacgg acgaataggc cacgagtttt ttcatgtcct tttgcacgat
    30241 ggccacgagg ccgacgtaga tcaccgctat cagcgacagg gtgatcatca gccaggccca
    30301 ttcatgcgcg gcgtcgggag cgatcggcag tgagaagcgc aggaagccat aggcacccag
    30361 cttcagcatg atggccgcca gcactgcgga accaccggtg ggcgcttcga cgtgcacgtc
    30421 gggcaaccag gtatgcaccg gccacatcgg caccttgacg gcaaaggccg cgaacaacgc
    30481 aaagaacaac aatgtctgcg cggtgccgcc cagcggcagc ttgtgccagg cagcgatatc
    30541 aaagctgcca cctgcctggt tgtacaggta gatgaaggca accagcgtca gcagcgagcc
    30601 cagcaaggta tacaggaaga acttgaacgc cgcgtagatc ttgttcggcc caccccacat
    30661 gccgatgatc aggtacatcg gtatcagcgt ggcttcaaag aacacataga acagcatgcc
    30721 gtcgagtgcg gagaacacac cgatcatcag tcccgacaga atcaggaagg ccgccatgta
    30781 ctggtagaca cgctcggtga tggactccca cgaagcgatg accacgatca cggtaatgaa
    30841 ggccgtcaat ggtacgaacc acatcgagat gccatccacg cccaggtggt agtgcatgtt
    30901 gaagcgcgcg acccaggggg ccttttcaac gaactgcatg gctgcagtac cggcatcaaa
    30961 cccgctgatc agcggaatcg ttaccgccag gcccaacagg gagccgatca gcgccagcca
    31021 gcgcacggta cgcgcatgct tgtcgcaacc gaacgccaga agcaggacgc caaaaacgat
    31081 gggcgtccaa atggcaagac tcaacaaacc catttgttat ttgctcctat ttgttgagcc
    31141 agacgaaata cgtcatgagg gcgaagacac ccaggatcat gaacagtgcg tagtggtaca
    31201 ggaaaccgga ctgcacacgc cggactgcgg cagaaatacg cgccaccagc ttccacgaac
    31261 cgttcacgat ggcgccgtcg atcaacccct ggtcaccacc cttccacaga accacaccca
    31321 gcaggcgcgt gccggctgca atgatgttct cgttgatcca gtccatgtag tacttgttct
    31381 cgagcaaggt gtagatgggc tgaaacacac gcttgatcgc cgccggcagc gccggattga
    31441 cgatgtacat gtagtaagac agcgcaacac ccgccagagc cagccagaac ggcgccgtct
    31501 gcaatgcatg cacggccatg gccacagggc cgtggaacat ctgcgccagt tgcgccatcg
    31561 ccgcatgctt ggagccgtcg acaaagatca cgtccttgaa gaagtcgcca aacagcatgg
    31621 gctggatgta catgaaaccg atcaccaccg aagggatggc cagcaacacc agcggcaccg
    31681 tcaccaccca tggcgactca tgtggcttgg aatggtcgtc atggtgatgg tgcggatcgg
    31741 gttcttcctg cgcctcatgg gcatcagggt tctggtcgta gcgctccttg ccatggaaga
    31801 ccaggaagta catccggaac gaatagaacg cggtgacaaa cacccccgcc atgaccgcaa
    31861 aatgtgcaaa gccggcagcc ggcagctggc tgaagtgcac ggcctcgatg atgttttcct
    31921 tggaataaaa gccggagaac agaggtgtgc caatcagtgc caacgagccg agcagcgagg
    31981 tgatccaggt gataggcatg tacttgcgta cgccacccat ccagcggata tcctggttgt
    32041 ggtgcatgcc catgatgacc gagccggcac ccagaaacag caaggccttg aagaaagcgt
    32101 gcgtcatcag gtggaacacc gcgaccgaat aggcggatgc ccccagcgcc acggtcatgt
    32161 aaccgagttg cgacagcgtg gagtaggcca ccacgcgctt gatgtcgttc tgaatgatgc
    32221 ccaggaagcc catgaacagg gccgtgatgg agccgatgac caggatgaag ttcagggcag
    32281 catcggacag ctcgaacagt ggcgacatgc gcgagaccat gaagatgccc gccgtcacca
    32341 tggtggcggc gtggatcagc gcagaaatcg gcgtcggacc ttccatggag tcaggcagcc
    32401 agacatgcag cgggaactgg gccgacttgc ccatggcgcc aatgaacaga cagatgcaga
    32461 tgacggtgat cagcatccag tcggtgccag gaaacaccaa ggcacccaac tcctgcttct
    32521 tggcaaacac ctcggcgtag tcaaagctgc cggcataggc cgcaatcagg ccgatgccca
    32581 ggatgaaccc gaagtcaccg acacggttga ccaggaaggc cttcatgttg gcgaagatgg
    32641 ccgttggctt gttgaaccag aaaccgatca gcaggtagga caccaggccc acggcttccc
    32701 agccaaagaa cagctgcagc atgttgttgc tcatcacgag catcagcatg gagaaggtga
    32761 acagcgagat gtaggagaag aaacggttgt agccctcgtc ttcctccatg tagccaatgg
    32821 tgtagatgtg caccatcagc gatacaaagg tcaccacgca catcatcatc gccgtcaggc
    32881 cgtcgatcag aaagccgacc tccatcttca ggccgccaac caccatccac gtatagaggg
    32941 tttcgttaag gcgtgcgcca tccacggcca cgctgtacag cgtggacgcg gagatcagga
    33001 aggacaccag cacgcccaga atggtcagcg tgtgcgagat gcggcggccg aaccagttgc
    33061 caccaaaggc cgtgccaaaa aggcctgcca gcagggagcc ggccaacggt gccaagggca
    33121 cagccagcag cgtcgaagca gaaagggttt gactcattct tgagaacctt gcaagtaccg
    33181 gttagcccat caacccttga gggtgttgag atcttccgca ttgatgctgt tcttgttacg
    33241 gaacaacagc accaaaatgg ccaagccaat ggccgcttcg gcggccgcca cggtaaggat
    33301 gaagaacacg aatacctgac cgtgcatgtc gcccaggtag tgcgaaaacg cgacaaagtt
    33361 catgttcacc gccagcagca tcaactcgat cgccatcagc agcacgatca ggttcttgcg
    33421 gttcaggaaa ataccgacca ccgccagggc aaacagcatt gcccccagcg tcagaaaatg
    33481 gcccagggtc aatgtcatgc cttgttctcc tctgcactga tatcgccgtc tgccaagggt
    33541 tgtgctgcct gcgtgaccgg catcttcacc acgaccagac gatcactagc gcgggcacgc
    33601 acctgggccg tgacatcgct ggccttgacg tccttgcgtt ggcgcagcgt aagggcaata
    33661 gcggaaatca tggccaccag cagaatcacg gcagcgatct ccaccggata cagatattcg
    33721 gtgtacatca gtttgcccaa tgcctgggta ttggaatacg gcaccacctg tccggccgca
    33781 ttgaccagag tcgcgacggc cttgggttct tccactccac ggaagccggt catcagcacc
    33841 gcacccattt caaacgccac cagggcacct atcagggcag ccaaggggaa atgcttccaa
    33901 aatccacgac gcactgcgtc gatacgaatg tccagcatca tcaccacgaa caggaacagc
    33961 accatcactg cccccaggta caccagcacc aaggcaatgg caaggaactc ggccttcaat
    34021 agcagccaca aaccggcagc ttgggaaaag gccagaatca aatgcaacac ggcatgcacg
    34081 ggattgcgcg ccgtgatcac ccggaaggcc gcgtacagca gcactaccga gaacaggtag
    34141 aagaatccgg ttttaacgtc catggatcgg gtcttttcaa aagttcaggt gtgatgcgtc
    34201 acccgatcaa cggtatttcg cgtccgccgc cttggccgcc gcgatctcgg gttcatagcg
    34261 atcgcccaca gccagcagca tttccttggt gaaatacagg tcaccccgtt tttcgccgtg
    34321 gtattcgagg atctgcgtct ccacgatcga atccaccggg cagctttctt cgcaaaaacc
    34381 acaaaaaatg cacttggtca ggtcgatgtc atagcgcgtg gtacggcgcg tgccatcggc
    34441 acgcacatcg gactcgatag tgatggccat cgccgggcac acggcctcgc acagcttgca
    34501 ggcgatgcaa cgctcttcgc cgttgtcgta acggcgcagc gcgtgcaagc cacgaaagcg
    34561 cggcgacaaa ggcgtctttt cttcgggaaa ctgaaccgtg accttgccac ggaaggcata
    34621 gcgcccggtc agcaccatgc ccttggccaa ttcccagagc atgaagctct tgaagaaatc
    34681 cttgagagaa aaagatgcag gagcaacaac agcagacatg aatggtgtcc ccgcttattt
    34741 ccaaatattc cagggcgaca gcaaccaggc gcccacgacc agcacccaca ccagggtcac
    34801 cgggatgaag atcttccagc ccagacgcat gatctggtca tagcgatagc gcgggaaggt
    34861 ggcgcggatc cagatgaaca tggataccac cacaaaggtc ttgatgccaa gccagatcca
    34921 gcccggaatg aagccgaggg cgtcgactgg cggcaaccag ccccccagga acatgatcac
    34981 tgccaggatg gacaccagcc acatggctgc atattcagcc aggaagaaga tggcaaagcc
    35041 catgcccgaa tattcgacca tgtgaccagc cacgatctcc gcctcgcctt ccaccacgtc
    35101 aaacggatgg cggttggttt cggcaacccc cgaaatcaga tacacgagga agatgggcag
    35161 caaaggcagc cagttccagg acaggaagcc caacccgatg ccagccatgg tgccgcgagc
    35221 ctgccccaac acaatctcgg tcaggttcat gctgcccgtc accatgatca ccaccacaaa
    35281 gcaaaagccc atggcgattt catagctgac catctgtgcg gaagcgcgca atgcgccaag
    35341 caaggcgtac ttggagttgg atgcccagcc tgcaatgatc acgccataaa cctctatcga
    35401 ggcaatggcc atgaccagca gcaatccggc gttgatgttg gccaaggccg tatccggcgc
    35461 gaaaggcacc accacccagg ctgccagggc cggcatgatg gccatgaccg ggcccaggaa
    35521 gaacagaccc ttgctcgcag ccgtgggttg gatcacttcc ttggtaagca gcttcagacc
    35581 gtcggccagg ggctgcagca ggcccagggg accaacccga ttgggcccaa ggcgcgcctg
    35641 catgaaaccc agcagcttgc gttcccacaa ggtgaggtaa gcgactgccc ccatcagcgg
    35701 cgccagcacg caaacgatct tgatcagatt ccagatcacc ggccagccca tgctggtcca
    35761 ccagcttgcg gcgaccaggt tcaagccccc gttgtaaatc gcgtcaatca tgcaacgact
    35821 ccttcacgtg ccgcacgtgc gtcggcggtc agttgcagcg ctggcgcacg acgcaccagg
    35881 ccatcgagct ggtaaatgct ggccaccaca ggcgcagccg ccggcccgtg tgccacgtcg
    35941 atggccgcct tggtggcatt gctcagttgc tgagaagtca actgctccaa gccggccgtg
    36001 gcctggttca gtacatcctg cgaggactca aactccacac ccggcagagc catgatgttg
    36061 gcgagcacgc gcaacacctt ccaggcggga cgcgtctcac ccagcgggcg cacgacggca
    36121 tggaaactct gcacccggcc ttcggcgttg acaaaactgc cggaggtttc agtgaacgga
    36181 gctatgggca gcagtacgtc gctgatgtcc atattcgttt tgaacggact caatgtcacc
    36241 accatgtcca cgtccgccag cccccgggcc gcattggcac ccgctgcgga gtcgaactcg
    36301 ggctcggtat tcagcaggaa caaggccttc acctgccccg ccagcatttg tgcggcattg
    36361 aggccgccct ggcgtggttg tgcgccgacg aactgggcac caacggtgtt cgccgcctcg
    36421 ctcagatagc cgaaggtggc accggttgcc tgtgcaatcc actgcgcgag tgccagcaga
    36481 ctggatgcct gagcgtggtg ggcggcggca ttgccaagca agatagcctt gcgttcaccg
    36541 ctgagcagtg cgcgggcaat ggccagcgcc tcatcgtttg ccttgccgga cacgaccggc
    36601 gccacagtac ccttttcttg cgccacggcg gccgcaacat cggccaaagc ttgcgtccaa
    36661 tcagcggatg ccactacgga ttgtgtcgca ggcaaagccc agtcatatgc catggcattg
    36721 atggcaaata ccttgcaacc cttgcgcgct gcctggcgga tgcgctgggc gaacagcggg
    36781 tgatccttgc gcaggttgga gcccagtacc agtaccgact gcaggtcggt cagtgccgcg
    36841 atgggcatgc cgagccagcg aaccccctcg ggtgtggcga actcggcgtg gcgcaggcgg
    36901 tggtcgatgt tgtcgctgcc cagcccacgc accagggtcg ctgccaggtg cagctcttcc
    36961 agcgtgctgt gcgggctgac caacgcgcca atggccgaag cgccatgttt gtcgcgtacg
    37021 catttcaaac cattcgccac atactccagc gccgtctgcc aatcgacctc ctgccactga
    37081 ccaccctgct tgagcatggg gcgctgcaga cgatcgtcac cattcagtgc ttcataggag
    37141 aagcggtcgc ggtcggcaat ccagcactcg ttgacggcct cgttctccag gggcacgacg
    37201 cgcatcacct tgtggttctt gatctgcacc acgaggttgg cacccgtgga gtcatgcggt
    37261 gaaatcgact tgcggcgcga caactcccag gtgcgggcgc tgtagcggaa gggcttgctg
    37321 gtgagcgcac ccaccgggca gatgtcgatc atgttgcccg agagttccga atcgaccgtg
    37381 tcacccttga cagtcgtgat ctccgaatgc tccccgcggt tgatcattcc cagctccatg
    37441 tcgccgccaa tctcctggcc aaagcgcacg caacgggtgc agtggatgca acgggtcatc
    37501 tcctcggtgg agatgagcgg ccctatgtcc ttcttgagaa ccacgcgttt ttcttcaccg
    37561 taacgcgagg ccgaagcgcc atagcccacg gccaagtctt gcagctggca ttcgccgccc
    37621 tggtcgcaga tcgggcaatc aagcgggtga ttgatgagca ggaactccat caccgattgc
    37681 tgcgccttga tggccttgtc actcttggtg cgcacgatca tgccctgggt caccggcgtg
    37741 gcgcaggccg gcatgggctt gggcgccttc tcgatgtcca ccagacacat gcgacagttg
    37801 gccgcgatgg agagcttctt gtggtagcag aaatggggaa tataggtgcc cgacttttca
    37861 gcagcatgca tgaccatgct gccctcggca acctccacct tctgaccgtc cagttcaatt
    37921 tcaaccatat gcttttctca cacgcgggcg gcggaggtgc cctgcttgac cgcggcctcg
    37981 atcttggcca cgaactcgtg gcggaaatgc ttgatcatgg cgcgcacagg catggcagcc
    38041 gcgtcgccca gcgcacagat ggtgcgcccc atgatgttga acgccacgtt gtcgagcagc
    38101 tcgatgtctt cctcgcggcc ctggccgtgc tggatgcggt gcaccacgcg ccacagccag
    38161 cccgtgccct cgcggcaggg tgtgcactga ccgcatgatt cgtgcatgta gaaataggat
    38221 agacgcacca gcgactcgac catgtcacgc gaatcgtcca tgacgatcac tgcgcccgaa
    38281 cccagcatgg agccggcctt ggcgatggag tcgtagtcca tggtgcagtc catcatgatg
    38341 gaagcaggca acacgggcga ggaagagccg ccgggaatca ccgccttgag ctggcgcccc
    38401 ttgcgcacgc cgcctgcgag ttcgagcagc ttggcaaacg gcgtgcccag cggaatctcg
    38461 tagttgccgg ggcgctcgac gtcacccgag accgagaaga tcttggtgcc tccgttgttc
    38521 ggcttgccgc attccagata ggcctgacca ccattcctga tgatccaggg cacggccgca
    38581 aaggtctcgg tgttgttgat ggtggtcggc ttgccgtata gaccaaagct ggccgggaac
    38641 ggcggcttga agcgcggctg tcccttcttg ccttcgagcg actcgagcag cgcggtttcc
    38701 tcgccgcaga tgtaggcgcc aaagccgtgg tgcgcgtgca gctggaagct gaaggcgctg
    38761 cccatgatgt tgttacccag gtagccggcg gcgcgcgcct cttccagggc ggcctcgaag
    38821 cgttcgtaga cctgaaaaat ttcgccgtgg atgtagttgt aacccagcga aatacccatc
    38881 gcgtacgcgg caatgatcat gccctcgatg acgatgtgcg ggttgaacat caggatgtcg
    38941 cggtccttgc aggtgcccgg ctcgccctca tcggagttgc acaccagatg cttctgccca
    39001 ggaaactgac gcggcatgaa gctccacttc agccccgtgg gaaaacccgc gccgccacga
    39061 ccgcgcaggc ctgattcctt gaccgtggca atcacctgat cctgcgtcag gccttccccg
    39121 ccatccttgc cgaggatctt gcgcagtgcc gcataaccac cgcgcgcttc gtaatccttg
    39181 atgctccagt tgccgccatt caagtccgcg tagatctgcg ggttgatgtg acggtcatgg
    39241 aagcaggtct gaacgcccgt ggcctgaaac tgcgacagga cttgtgcgac cgtggtcatt
    39301 gctggccctc cgcagcgcgc aagccgtcga ccaattggtc gagcttgtcg ttgtccatga
    39361 aactgcacat gtgacggtca ctcaccagca tggccggcgc gtccgcacag gcacccaggc
    39421 attcgcattg ctgcagggtg aacatgccgt ccggcgtggt ctctcccatc tcgattccga
    39481 gcttggcttc aaggtgatgc agggcctggt tgccgccacg cagctggcac ggcaaattgg
    39541 tgcacaccgc cagcttgtac ttgcccacgg gttgctggtt gtacatgttg tagaaggtcg
    39601 tgacttcatg cacggcaatc tcgggcatgc ccaggtagtc ggcaatcacg gcttcgtttt
    39661 cctggctgac ccagccctgc tcctgctgca cgatggacag gcaggccatg acggcggact
    39721 gcttctgttc aggcgggtac ttggctactt cgcgcgcaaa gcgcgccttg gtcgcttcgg
    39781 taatcatcgg tcaatctctc cgaacacgat atccatggtg ccgatgacgg ccacggcatc
    39841 ggcaatcatg tggccgcgcg tcaactcgtc atatgccgac aggtgggcaa acccaggggc
    39901 gcggatcttc aggcgatatg gcttgttggc accatcactc acgatgtaga tgccgaactc
    39961 gcccttgggg tgctcgaccg cggcataggc ctcgccctcg ggcaccttga agccctcgga
    40021 gaacagcttg aagtggtgga tcagatcctc catgccggtc ttcatgcgct cgcgcggcgg
    40081 gggtgccacc ttgtagttgt ccacaatgac cgggcccggg ttggcacgca gccacttcgc
    40141 gcattgctga atgatgcggt tggactggcg catttcttcc acgcgcacca ggtagcgatc
    40201 gtaacagtcg ccgttcttgc ccagaggaac gtcgaaatcg acgcggtcgt aagcgtcgta
    40261 gggttgcttc ttgcgcaaat cccaggctac gccggagccg cgcagcatca caccggtcat
    40321 gcccagattc agggcgcgct cggccgagac cacgccaatg cccacggtgc gctgcttcca
    40381 gatgcggttc tcggtcagca gggtttcgta ttcatccacc aggttgggga acttggcgca
    40441 gaagtcctcg atgtagtcca gcagcgagcc ctggcggttg ctgttgaatt gctccgtggc
    40501 cttggcactg cgcaccttgc tgggcttgag ttgcgccatg ctgtcaggca ggtcgcggta
    40561 caccccaccc gggcgatagt aggcggcatg catgcgcgcg ccagacacag cctcgtacat
    40621 atcgaacaag acctcgcgct cgcggaaggt gtacatgagc acggtggatg cgcccaaatc
    40681 cataccattc gagcccagcc acatcaggtg gttgagcagg cgcgtgatct cgccaaacat
    40741 ggtgcgaatg tacttggcgc gcagcggtac ctcgataccc agcagcttct ctatggccag
    40801 gcagtaggcc tgttcgttgc acatcatgga cacgtagtcc agcctgtcca tatagggcaa
    40861 cgactggata taggtcttgt gctcggcgag cttctcggtc gcacgatgga gcaggcccac
    40921 atgggggtcg gcacgctgca ccacctcgcc gtcgagttcc agcaccaggc gcaacacacc
    40981 gtgcgcggca ggatgctgcg gcccgaagtt cagggagtag ttcttgatat cagccatgtt
    41041 caaacacctg ggcagcgcgc cgcctcagtg caggcctccg tatttttctt cgcggatgat
    41101 gcgcggagtg atctcgcgcg gctctatggt cacgggctcg tatatcacgc gacgctgctc
    41161 ggggtcgtag cgcatctcca catgacccga cagggggaaa tccttgcgga atggatggcc
    41221 gatgaaaccg tagtcggtca ggatgcggcg caggtcgttg tggccatcga aaacgatgcc
    41281 gaagaggtcg aaagcctcgc gctcgaacca gttggcgccg ctccagatgt cggtcacgga
    41341 gggaatcacc gggaagtcgt catccgggca gaacaccttc acgcgcacac gctgattcag
    41401 cgacaccgac agcagatgcg acaccaccgc atagcgcaag ccgtctgtgc ctacttcacg
    41461 gtatgtcgaa tagtccacgc cacacagatc gatgagctgt tcgaaacggc attgcggtgc
    41521 atcacgcaaa gtctgcatga catccagata gtcaccagcg gcaaccacga cggtgagctc
    41581 atcgagcgcc agcgcgatat cgcgcacctt gtcgcccagc gcggccgcca cggcctcgcg
    41641 cagcttctca gggtgaatgg caaagtcggt catcaatacc cctcaaacac gcgcaatggt
    41701 ctgggtgcgg cggatcttct gctgcagctg gatgatgccg tagatcagcg cctcggccgt
    41761 aggcgggcag cccggcacgt acacgtccac cggcaccacg cggtcgcagc cacgcaccac
    41821 ggaatagctg tagtgatagt agccaccgcc attggcgcaa gagcccatgg agatcaccca
    41881 gcgcggctcg gccatctggt catagacctt gcgcaaggcg ggcgccatct tgttgcacag
    41941 cgtgccggca acaatcatca ggtcggactg gcgcgggctg gcgcggaaca cctcggcacc
    42001 aaaccgcgcc aggtcatagc gcgccgccgc cgcatgcatc atctccaccg cacagcaggc
    42061 gaggccgaac gtgacgggcc aaatggatcc ggtcttggcc cagttcacca cagcgtcgta
    42121 gctcgtggtg acaaagcctt ccttcatcac accttcaatc attgcgtcat tccttgtgta
    42181 gcgccgctca ttcccaatcc agggcgccct ttttccactc gtaggcaaaa cctatgacca
    42241 ggatggccag gaaaatcacg accgccacga aacccgtcat ccccacatcc tgcaaggcca
    42301 cggcccaggg gaacagaaag gcgatttcga ggtcaaacag aatgaacaaa atggccacga
    42361 ggtagtagcg cacatcgaac ttcatgcgcg catcgtcaaa ggcctcgaag ccacattcgt
    42421 aaggggagtt ttttgccggg tcggggcgat tggggcccag cacatagccc aacacaaggg
    42481 gcacgacgcc gacaccgacg ccaaccaaga tgaacaagag aactgggagg tattgatcga
    42541 ggttcatcta tgccgctctc gccggtgaaa ccgcctcaca cagctcgcac tgcgggcccg
    42601 tgaggcggcc tttttatttg gtgccgtcgg cgagactcga actcgcacag ctttcgccac
    42661 taccccctca agatagcgtg tctaccaatt tcaccacgac ggctgctgta tgcatctgga
    42721 tggcttgagg accgatggga cggtttttgc tttccagaca gccggggatt ctaccccgga
    42781 aaaaccctgt cacaaagcat ggaggcgctt ttttgctacg cctcttgcat gctcatttgg
    42841 ttgggatttg cgccgcacca gatgccggtt ccgccgctgg tgcgggcacc gctgcatcgg
    42901 ctggcgctgt gctgctggcg ggcactgctg cggccggtgt ctccagcacg ctgcccgtgc
    42961 ttggcgcacg ctgattgccg aaataggcca gggccagcgt ggccacgaag aacacggtag
    43021 ccagggccgc cgtgctacgc gacaagaagt tggcgctgcc gctggcgcca aacaggctgc
    43081 cggcgctgcc actgccaaac gcggcgccca tgtcggcacc cttgccatgc tggatcagga
    43141 tcaggccgat catgcccagg gccgtcagca tctgcacggc cagaatgatg ttcacgattg
    43201 cattcatgct tggttagctc ctcaactgat agctgcttac gctttattgg tctgcgctgc
    43261 agcaataatt tgcaaaaagt cttcggcctt gagcgaggca ccgccgacca ggccaccatc
    43321 gatgtccggc tgcgccagca actgcgccgc attcgccgca ttcatgctgc cgccatacag
    43381 cagcggtatg cgcgctgcct gctcggtggc ggccgccagt tgggcacgca gcacggcatg
    43441 cacggcctgg gcctgctccg gcgtcgccgt gcgaccggtg ccaatggccc agacaggctc
    43501 gtaggcgacc acaatctcgc tgatgcaatg gccgttggcc tggatcaccg ccgccagctg
    43561 gcgcttgacc accgcctcgg tctgacccgc ctcgcgctgc tccagcgttt cacccacgca
    43621 gacgatgggc gtgatgcccg cggccagcgc acgctgagcc ttggcggcca ccacggcatc
    43681 ggtttcgcca tggtattggc ggcgctcgga atggcccacc agcacatagc gcgcgccgaa
    43741 gtcgcgcagc atggccgccg atgtctcgcc cgtgtaggca cccgattcat gctgcgagac
    43801 atcctgcgcc gccagcgcaa tgcttgatcc cgcgaccagc gcctgcacct gcgccaggta
    43861 gggcgccggc acggccacgg cgacgttgca ggccggctgg cccaggccgc cacgcaagcc
    43921 ctgcagcagt gcggcgttgg cctgcaggct gccgttcatc ttccagttgc cggcgatgag
    43981 tttctttatc tgagtcatgt cgtccacctc aaaacaatct tgcctatatg ttggttcgac
    44041 tccatcagcg catgtgcctg ggccgcatcg gccgcggcaa aggtgctgtg aatgacgggc
    44101 ttgatggctc cagacgccag cagcggccag acctgctcgc gcagggcctg ggcgatcgcc
    44161 gccttgaaag ccacagggcg cgggcgcagc gtggagccgg tgatcaccag gcgcttgcgc
    44221 agcaccagcg cggcgttgaa ctcggccttc acgccacctt gcacggcaat gatgacgatg
    44281 cgcccatcct cggccaggca ctccacctcg cgcgccacat aggggccggc gaccatgtcc
    44341 agcaccacat ccacgccacg cccctcggtg atgtgctgca cctcggcgac aaagtcctgc
    44401 gcctggtagt tgatggcatg gtgcgcgccc agcgccagac aggcggcgca tttttcgtcg
    44461 ctgcccgcgg tgatgatgac ggtggcgccg cgcgcacgcg ccagctgtat cgccgtgacg
    44521 cctatgccgc tgctgccccc ctgcaccaac agggtctcgc ctgcctgcag gcgggcgcgg
    44581 tcgaagacat tgctccacac ggtgaagaag gtctcgggca gcgaggccgc ctcgacgtcg
    44641 gacaggccag cgggaaccgg caggcattgc gccaccgggg ccacgcaata ttcggcatag
    44701 ccgccaccag ccaccagcgc gcagacgcga tcgcccacag cgaagccggc cgcggccagg
    44761 gccgccgcgt cacccgactc gatgacgccc gcgacctcca gccccggcag gtccgaggca
    44821 ccggcaggcg gtgcgtaatg ccccttgcgc tgcagcacat cggggcgatt gacaccgctg
    44881 gccgccacgc ggatgagcag ctcgcccacc cccgccatag gcactgggcg ctcgcccaaa
    44941 cgcagcacct caggcgcacc aaaggccgtg atttctaccg cttgcattgt caattcaccg
    45001 cataaatttc aagccaaaat ggctgcttgc gcccactaag caagcgcaag cagctcattt
    45061 ttcaatagca actgccacgc ggcctgctta ctcctgcgtc ggctgatcct gctcgccacg
    45121 ctcggcgcga ggctcacgcg gctcgcggtt ttcacgcggc gcgcgcgggg cacgctcgcg
    45181 gtactcgcgc ggctcacgcg gttcgcgctc gggcatgccg gcggggcgct caatcagtgc
    45241 cttcatcgac agcttgatgc ggcccttgtc gtcggcctcc agcgccttga cgcgcacgat
    45301 ctggccttcc tgcaggtagt cggtgacctt ttccacgcgc tcgtgcgcga tctggctgat
    45361 gtgcagcagg ccatccttgc ccggcagcac gttgaccagg gcgccgaagt ccaggatctt
    45421 ggtgatcggg ccttcgtaga ccttgccgat ttcaacttcg gcggtgatct cctcgatgcg
    45481 gcgcttggcc tcgtcggcct tggcggcctc ggtggcagcg atggtgatgg tgccgtcttc
    45541 ctcgatgttg atctggcagc ccgtttcgtc gcacagcgcg cggatggtgg cgccgccctt
    45601 gccgatcacg tcgcggatct tctcggggtt gatcttcatg gtgaagagct tgggcgcaaa
    45661 gctgctgatc tcggccttgg cctcgcccat ggcttcctgc atcttggcca ggatgtgcat
    45721 gcgcgcttcc ttggcctggg ccagggcgac ttgcatgatt tccttggtga tgccctggat
    45781 cttgatgtcc atctgcagcg ccgtgatacc ggcggtggta ccggccacct tgaagtccat
    45841 gtcgcccagg tgatcctcat cgcccaggat gtcggtcagc acggcgaact tgttgtcctc
    45901 tttgatgagg cccatggcga tacccgccac atgcgccttc atgggtacgc ccgcgtccat
    45961 catcgacagg cagccgccgc agaccgaagc catggaagac gagccgttgg actcggtgat
    46021 ctcggacacc acgcggatgg tgtaggggaa ctcttccttg ctcggcagac aggccaccag
    46081 ggcgcgcttg gccaggcggc cgtggccgac ttcgcggcgc ttggtcgagc ccatgcgacc
    46141 cacttcgccg gtggcaaagg gaggcatgtt gtagtggaac aggaagcggt cttcgaactc
    46201 gccagccagt gcgtcaatgc gctgcgcgtc gcgctcggtg cccagcgtag acaccaccag
    46261 cgcctgcgtc tcgccgcgcg tgaacagggc tgaaccgtgg gtgcgcggca gcacgctgtt
    46321 gcggatttcg atagcacgca cggtgcgcgt atcgcggccg tcgatgcgtg gctcaccggc
    46381 cagaatctgg ccgcgcacga tgcgcgcttc gatgtcgaac agcatgttgt ccaccttgac
    46441 gccgtcaa
//
LOCUS       DAOMTQ010000006        42685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_48_length_42685_cov_26.512128, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000006 DAOMTQ010000000
VERSION     DAOMTQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 42685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42685
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48_length_42685_cov_26.512128"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..298
                     /locus_tag="PK518_01445"
     CDS             65..298
                     /locus_tag="PK518_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47603.1"
                     /translation="MLPDGRILLVGQGSVVATSSDGGHHFSFSRVAGRATLTDIALDS
                     EGAGWITSDAGLQPYQVAAKGESSNLSSAVAKP"
     gene            427..2319
                     /locus_tag="PK518_01450"
     CDS             427..2319
                     /locus_tag="PK518_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47604.1"
                     /translation="MFALPCAGLCLYAAAQHPVAPMLALSLLAAALVWAMWRPVDLWF
                     VLPALLPVASFAPWTGWWVVDESDLLVLALLGGAYGRWALDAWRVPAAVHGTTPAGIR
                     WAYWVLPPLLVVGVWRGLDDARGAASWVALLADLWAQGPHGDYDQPGNTLRVAKSLAW
                     GLLLMPALYRSSRGAALRLARGMLVGLFFVCIAVLWERGLYVGWLDFSANYRTVAWFW
                     EMHVGGGAIDAYLAMALPFAWWAAWSAPHGWRWYAAAGLVLLATYAVLTAYARGLYLT
                     VVITAVGMAWLAHRHRLKAPDATLWHRRAMAWLLVALVVEILAVWVGGAFMSDRLGRS
                     NADLHQRLAHWKRGVHMLHTPGQWVLGLGLGRLPAQYSAQNPEGALPGRVRWVRHGDA
                     GNEVWLYGPQRADAKGGLALTQHVPLESGGHYTVRLRAHMNAPMWLKVQLCEQYLLYQ
                     ARCQLRTRQVSEASKTTDGWIVLPLLGPALASGGRGTVSRDGVLSVQLLQANASIRIT
                     AVELLDKSGRQVLKNADLALGPRYWSSIAYGNFLPWHMDNLLLELLIERGLLAVLILA
                     LAVAWALLQLARGVRRGSPLALVVGGSIIAGLMAGGLISFAEVPRVSLVLWLLPIVSF
                     CITEDR"
     gene            2475..3869
                     /locus_tag="PK518_01455"
     CDS             2475..3869
                     /locus_tag="PK518_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HRM47605.1"
                     /translation="MVRIFNHYFRQRSILQAMVYMVLIVSTLVISMAWQVGAEALNGA
                     IISSGVIRGGLIAVGILGMNAALGIHESPSGLTVGQVRSRAVLSFILSMVIAAGVLLL
                     LPLSPPYHNEQTLLALLIMVSAMLILRLLGNSLLPASYKRQRVLVYGTGSRACVVTES
                     LMKNDPSIELVGHFRGPQEKEQVASGLHVVSAGKTLTEVVEEYRVNEIVVAVNERRGG
                     SMPLRELLDCKLQGVQVIDLATHFEKLLGQVHLEAVSAGWLIFGDGFKQGVVRSLVKR
                     LLDVVGSAVLLLLAAPVMLVTAVLVFLESGLPIFYRQERVGLNGQTFNVIKFRSMRQD
                     AEKDGTPRWAGAGDARVTRVGKFIRKTRIDELPQLLCVLKGDMSLVGPRPERPYFVER
                     LTQDIPYYALRHGVKPGLTGWAQVRYHYGASVKDAEQKLQYDLYYVKNHSWFLDLIVL
                     FETVGVVLTGKGAQ"
     gene            complement(3882..6191)
                     /locus_tag="PK518_01460"
     CDS             complement(3882..6191)
                     /locus_tag="PK518_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 and HATPase_c domain-containing protein"
                     /protein_id="HRM47606.1"
                     /translation="MTLSAQAGYHRNLRREWLVLSLLMLGLVAWLCLAGGLRRMDYLV
                     QDAGTRLYAHAAHPDIVIVAVDDRSIASIGRWPWRRALHAQLVDQITAQSPRALGLDI
                     LFGEEDADYPGDDLLLARALERNAHAVLPVARRGDGPRSSADAPMPLLRQAAAQLGHV
                     QVQLDEDGVARSLFEHEGPASAPWPHFSTAMLCAEGLALASCRGNAAPVNAPWTSQDL
                     QILRFAQGQPAFTTYSYIDVLTGQLPAGALRDKYVIVGATATGLGDMFAAPMVSQAER
                     IPGVEVIAHALNADLMRWHVQRAPEAWNLAFNLTPVALTLLAIVLLGPLAGLIASATL
                     FLATLVAAAGAPTLSGWQFAAAPALAGIAAVYPLWSWRRLSAAAHFLQLEIQALRGAG
                     LSPLPDEAMPLVLRGDLLEQRIHAVEDATRRLRKLHHFISDSLQHLPSPTFVCDARGR
                     VTLANEAALLYLPSGQAAQGQAIAQLLVELVHPHSGRPLLPQDASRMVELPAQQEGRD
                     AQGRHMLMLCKPFALEGSTIWLITLVDLTDMRRAQQQRDQALHFISHDIRAPGASILT
                     LLEMRREFPEQLSQQDLLARIERHARSSLAMAQGFVQLASAQAYEYHSTPFDLAAALE
                     ETVDDAWASAQDQQVQMHITHLPQAAPVHGDRALICRAITNIVGNAIKFSPPGSTVQC
                     ALTLESPYWVISVRDQGPGIAPEQQSHLFQPFKRLHEGSHPGIAGVGLGLALVQTVLQ
                     RHGGLVQVRSSSGAGAEFRLLLPQETAEQ"
     gene            complement(6232..7923)
                     /locus_tag="PK518_01465"
     CDS             complement(6232..7923)
                     /locus_tag="PK518_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRM47607.1"
                     /translation="MQATPMPHRYARQTLPLALALCAALAHGGALAQPLDIEHQVQRG
                     DTLEALSTHYLGTPRLWPQLQRHNQVANPRHLQPGSVLHIPMQLLPLGSAEVTFVQGE
                     ATVTAPTNASATTLQAGQPLPEGARVQVAPNSFVTVRLADGTLIRVHADSDLQLQQLR
                     RRGRAGDAQSVLELRRGSVETSVPANSGGARRFEIRTPKASTSVRGTRFSVALTGDER
                     ALTAVTEGTVAVQPHATRQGASIPAGQGVVVAADGELGAPQPLLPAPDLSALPAAVHD
                     ADFLSLALPPVAAAVAYQVRVARDADFTTVVRDGAFSGPQARMRAVDDGSYHLAVRAV
                     DSTGLPGLVAQRTITVKAHPVPPLYQAPPQGATISRTQGELLCTPVLGAVRYRIQVAT
                     DAAFTAPALDETRDQQCGAQVQALAPGNYFWRAASVRELPGGGGDQGPYAPAQPFSVA
                     NNPNAPDAAALQVGNEGPALQLRWPGGSGQSYRLQLASSQDFAALLMDERLGTAAWTA
                     ASLAPGEYFVRIQTRDPSGLESEFSTPRLIHVRAAVQSSGGLPVTSSDGRPLARP"
     gene            complement(7994..8722)
                     /locus_tag="PK518_01470"
     CDS             complement(7994..8722)
                     /locus_tag="PK518_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM47608.1"
                     /translation="MRIAALDDDPLQLEFFTHALTAMGHACRTYTTGAALLKDLRRET
                     FDLLIVDWQLPDIEGPEIITWVRQQLDTELPILFVTHRQSESDIVQGLGCGADDFMVK
                     PVRLAEFKARVGALLRRAYPNDAQDVLEFGRFRFLPEARGLEMDGKPVELKAREYELA
                     LFLFQNMGRLLSRDHLREVIWGHSAEVISRSLDTHVSRLRSLLDLRPANGYMITAVYG
                     VGYRFEAVKPTAEQTANGSASQAQ"
     gene            complement(8906..10210)
                     /locus_tag="PK518_01475"
     CDS             complement(8906..10210)
                     /locus_tag="PK518_01475"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRM47609.1"
                     /translation="MTTTRFLTGITPSGTPHLGNYAGMMRPAIAATRTPGVENFYFLA
                     DYHALIKCQEPERIHRSTLEIAASWLACGLDPEQVTFYRQSDVPEIPELHWLLTCVTG
                     KGLLNRAHAYKAVQDKNAEAGREADDGVSAGLFMYPVLMAADILAFNAHKVPVGRDQI
                     QHIEMARDMASSFNHLYGEHFTLPEAAIEDNVATLLGLDGRKMSKSYDNTIALFCPPE
                     QLKKLIGSIVTDSRAPGEPKETEGSALFQLYQAFATPEETAAMRRAFAEGIAWGEAKQ
                     LLFARIDREIAPMRARYDDLMLHPEKVEAALQIGAQRARALAQPLLQRLRAAVGLRPL
                     SATRPQAQGAHKAGKAQLPAFKQYREADGKFYFKLVASDGRLLLQSTAFDAPRDAGQA
                     IARLQKEADALQALAAQLAPVAGVDAAEVTAALQALAAAADA"
     gene            complement(10215..10883)
                     /locus_tag="PK518_01480"
     CDS             complement(10215..10883)
                     /locus_tag="PK518_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRM47610.1"
                     /translation="MDLTNLIQTVLIYALPVLFAITVHEAAHGYTARHFGDNTATMMG
                     RVTLNPIKHIDPVGTVLMPLVLYFATSGAFLFGYAKPVPVNFHHLRNPKRDMVWVALA
                     GPASNFAQAIGWALLLVLLATLGGQERFFIEMARAGIAVNLVMWAFNLFPLPPLDGGR
                     ILVGLLPWRQAQLVARIEPYGFFIVMALVVGGIVGTLWLRPLMSLGYAAINLLLLPIT
                     ALLR"
     gene            complement(11053..11688)
                     /locus_tag="PK518_01485"
     CDS             complement(11053..11688)
                     /locus_tag="PK518_01485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRM47611.1"
                     /translation="MAQYFEIHADNPQPRLLKQAAALLSGGAILAVPTDSSYALVCRL
                     DDKDAVDRIRRLRQINDKHHLTLLCRDLSELASYAHVDNRQYRLLKLATPGAYTFILD
                     ATKEVPRRVSHPQRKTIGLRVPDRKGLQMLLELHGAPLLATTLIPPDETEPLNDPQEI
                     RERFEKQLDAIIDAGACPLEPTTVLDLTPMNTGGDPVVVREGRGSLQALGL"
     gene            complement(11699..12565)
                     /locus_tag="PK518_01490"
     CDS             complement(11699..12565)
                     /locus_tag="PK518_01490"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HRM47612.1"
                     /translation="MSHLLNADLHCHSVVSDGTLTPEALAARAHGNGVQLWALTDHDE
                     IRGQHRAAAAAQALGMDYLTGVEISVTFVDTTVHIVGLGFDADDAQLARGLAATRDGR
                     GERAQEIGAQLARVGIRGAYEGALCYVDNPELISRTHFARYLVDTGHCRDAGEVFRKY
                     LTEGKPGFVPHRWARLGDAVRWIREAGGMAVIAHPARYRLTANEEYALFSEFQQHGGQ
                     GVEVVTGSHTAAEYATYAAMAQEFGLAASRGSDFHSPGESHTDLGALPGLPGQLTPVW
                     QLLHERILRAAR"
     gene            12777..14513
                     /locus_tag="PK518_01495"
     CDS             12777..14513
                     /locus_tag="PK518_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM47613.1"
                     /translation="MNLNHLPVARKLWALVLGLMISMLALLAGLLSYSMHIDDDAARV
                     VHFNEERIGLALRWKSLSSLAVDRVVNALSSNDEELTERLVKQSKEGIQAIDAVQKQI
                     ETALFSPADKEQFQRVLAARKQVLEHNAEAGKLRANLDHTGARAVLDGKMLPAVERYV
                     AEQDAFVQLQERQRDAAKQRAADQRQRAIWLGVGIALVVVLLGMVLAALLVRSITQPL
                     ARAVGLADTIAAGDLTQDVHDERRDELGQLLRALSAMGARLRSVVGEVRSGVESVSAA
                     SSQIATGNHDLSARTEQTAANLEETAASMEELTATVTQSADTARQANQLAANAAQAAE
                     QGGQVMDQVVTSMQQITDSSRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRSLAQRSAEAAKEIKQLIGTSVDNVESGSAQVAQAGQSMQEIVQGVRRVSD
                     LIGEITASSTEQRDGISQVNQAVANLDQMTQQNAALVEESSAAAAAMHEQAQRLAQVV
                     AVFNVGSGAAVAARPAAKAAMPAPQAKAGAASARPQIAKPAPKPVAAAPARIASAKAP
                     VAAGGDDDWESF"
     gene            14626..15228
                     /locus_tag="PK518_01500"
     CDS             14626..15228
                     /locus_tag="PK518_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRM47614.1"
                     /translation="MLSTTRSSAASRPLPSMIFASRWLQLPLYLGLIAAQAVYVFHFW
                     VELIHLLEAALGDEQALQALVTSIGYKTDAPLTSLNETVIMLVVLALIDVVMISNLLI
                     MVIVGGYETFVSRLHLEGHPDQPEWLSHVNASVLKVKLSTAIIGISSIHLLKTFINAA
                     NYDEKVLMWQSIIHIAFLLSALAIALTDRLLSHNHGGNAH"
     gene            complement(15305..15754)
                     /locus_tag="PK518_01505"
     CDS             complement(15305..15754)
                     /locus_tag="PK518_01505"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HRM47615.1"
                     /translation="MLPVAVTEETFRHNGIISAQDAQELPVAKLREIFGADAALYLDV
                     RQYGSVYAVLKSETKVTLTARLVDLRTGDQLWSGEATASSAETKSTSGNPLGMLISAL
                     IDQIVDSLSDRSVQIAEIANGRLLYAGRPGGLLHGPRSPRYGADGQP"
     gene            complement(15736..15909)
                     /locus_tag="PK518_01510"
     CDS             complement(15736..15909)
                     /locus_tag="PK518_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47616.1"
                     /translation="MRSPISIGEVANRSNFQGGIFSDGVDRLGGQAQTILVTHLQQSG
                     RWPSPATTCCRWP"
     gene            complement(15995..17716)
                     /gene="recJ"
                     /locus_tag="PK518_01515"
     CDS             complement(15995..17716)
                     /gene="recJ"
                     /locus_tag="PK518_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRM47617.1"
                     /translation="MKIISRDIPPRAVWALEQAGVHPLLARLYAARGIRAQDELDDAL
                     ARLLPPGGLKGIGAAATLLADAIAQDKRLVIVADYDCDGATACAVGVRGLALLGARHV
                     QYLVPDRVVDGYGLTASIARRVAERGADMLITVDNGIASVEGVGEAKALGLSVVVTDH
                     HLPGPALPAADAIVNPNQPGCTFESKSMAGVGVMFYLLLQLRAELRARGRFDQTSQPR
                     LEPLLPLVALGTVADVVRLDNNNRRLVAQGLKRIRAGQMQPGIQALFDAAGRKAPAAT
                     TFDFGFALGPRINAAGRLADMTLGIECLLTDDAARAAELARQLDAINRERREIEGGMR
                     EQAMQLAEGLFDEGEEPPPAISVFDPDFHEGVVGIVASRLKDRLHRPTFVFAASSAPG
                     KEHELKGSGRSIQGFHLRDALDLVAKRHPGVILKFGGHAMAAGCTVAEEHFETFEQAF
                     AQVAQEWLDAATLTRRLETDGPLAPEYCRAELVDQLQLQVWGQGFAPPTFSEQVQVLS
                     QRLVGEAKNHLQLKLLHQGQPMDAIWFGHTEQLPERALLAFRLDVNEWRGERRVQFLV
                     EGVELPA"
     gene            complement(17713..18672)
                     /locus_tag="PK518_01520"
     CDS             complement(17713..18672)
                     /locus_tag="PK518_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HRM47618.1"
                     /translation="MPPMPHLIVALATSPTQGCQQAMKNLALPQLDRLLARLSPAGLD
                     AGNETDFSPPHERALARHLGLAAGPAGTTPWAAWHRLQTPAHMPTDHGLAWAFITPCQ
                     WQADANHVLLADPAQLQLTEDDSRSLLAILAPWFAEDGITLVYDQPTRWLAHGAAFDG
                     LATASLDRVLQRDVSSWLPDKLQARNLHRLHSEMQMLLYTHPFNDARAARRLPPVNAF
                     WVHGAGALPQPPVARHAPQLPNGLLQAALHEDWGAWSRAWAELDAGPVAQLAAHVGRG
                     GSARLTLCGERSAMTWHTAPRGLGQRIQSLFRPRRFADVREQL"
     gene            18762..20015
                     /locus_tag="PK518_01525"
     CDS             18762..20015
                     /locus_tag="PK518_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRM47619.1"
                     /translation="MQIPYELALGWRYTRAGRATRRNGFISFISGVSMLGIALGVAAL
                     IIVLSVMNGFQKEVRDRMLGVVSHIEIFAPGGAALPDMQRTLEEARANPNVIGAAPFV
                     AAQGLLARGEDMKGVLVRGIDPAREPEVTDLAAENVAGLKALVPGEFRVFLGGELARQ
                     LGVQVGDTVTLIAPSGQVTPAGVLPRLKQMTVAGLFNSGHYEYDSALVLMAHQDAERI
                     FRLEGPTGVRLKLKDLHQAPEVARQLARSLSGDLLIRDWTQQNKTWFAAVQLEKRMMF
                     IILTLIVAVAAFNLVSTLVMTVTDKRADIAILRTLGASPRSIMGIFVVQGAMVGVVGT
                     LAGLLLGLAIAFNIDVIVPAIERALHTRFLPQDIYLISRMPSDPQSSDIVPIAVISLI
                     LAFVATLYPSWRASRVNPAEALRYE"
     gene            20008..20688
                     /gene="lolD"
                     /locus_tag="PK518_01530"
     CDS             20008..20688
                     /gene="lolD"
                     /locus_tag="PK518_01530"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HRM47620.1"
                     /translation="MSDHQIVLQAEGLTKRFTEGRLDVSVLQGVDLQVRAGETLAIVG
                     ASGSGKSTLLHLLGGLDAPTAGSVTLKGQALGALKPGEQGRLRNAHLGFIYQFHHLLP
                     EFSAQDNVAMPLRIRRAPYAHCLEQARQMLAAVGLAERVHHRPAELSGGERQRVAIAR
                     ALVTRPACVLADEPTGNLDRATADGVFQLMLQLAREQGTAFVLVTHDEQLAARCDRVL
                     RLVSGVLG"
     gene            complement(20743..21789)
                     /locus_tag="PK518_01535"
     CDS             complement(20743..21789)
                     /locus_tag="PK518_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRM47621.1"
                     /translation="MVFLWPSLLWLLLILPLLVLLYLWLLRRRKVQALAYPGLALVRQ
                     ALGSRAQWRRHLPPLLFLLGLAALLLAAARPLAVLKLPSEQQTIILAMDVSGSMRATD
                     VEPDRLTAAQNAAKAFIKELPRHVRVGVVAFAGTAQLAQLPTQSHDDLLKAIDSFQLQ
                     RGTATGNGIMVALATLFPDAGIDIAALGGRQAMRVLPMDQVTRPEPEQKTFTPVAPGS
                     YRSAAIIMLTDGQRTTGVDPLEAAQWAADRGVRVYTVGVGTVQGEVIGFEGWSMRVRL
                     DEDTLKAVALRTNAEYFHAATAQDLRKVYETLSSRISVETKETEVTALMALAGAALML
                     LAGALSVWWFGRVL"
     gene            complement(21806..22741)
                     /locus_tag="PK518_01540"
     CDS             complement(21806..22741)
                     /locus_tag="PK518_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRM47622.1"
                     /translation="MPEARHAAPPAERLLQRLEWKVLRRLDGLLQGGARTLLRGAGID
                     LADLREYQPHDDVRHIDWNVTARLATPHVRVYTEDRDITAWFLLDLSPSVDFGPPGQA
                     KRDMLAGFVGVLARLIQRHGNRVGAVLHDGHAAMGRVLPAGGGRRQVLQLLHLLVGGQ
                     GAADPATPPGTTDLALLLNQAQGLLRRRSAVFVISDFLSQPGWDKPLARLARRHDVVA
                     VRLLDPLELELPDIGLLLLRDPETGEQLQVDTHERGFRHRFARLAAQREAELRASLAR
                     AGVDTLELSTDEDLLSALLRFMQLRGHRQHLAARA"
     gene            complement(22734..23753)
                     /locus_tag="PK518_01545"
     CDS             complement(22734..23753)
                     /locus_tag="PK518_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM47623.1"
                     /translation="MQTTHTTATLMEQILYQVKRVVVGQDRFLERVMVAMLAQGHLLV
                     EGVPGLAKTLTVKTLANCIQGQFKRIQFTPDLVPADLIGTRMYNQRTGEFSTALGPVF
                     THLLLADEINRAPAKVQSALLEVMQERQVTIAGQTHPVPEPFVVMATQNPIETEGTYQ
                     LPEAQVDRFMLKVLIDYPSEEEEFVIAQRALAPPEQVAAVASTEQLAALQKECRKVYV
                     DPALIQYAVRLVAATRAPRQHGLPDLAGHIAYGASPRATIALAEGAQALAMLRARAYA
                     LPEDLLDLVPDVLRHRISLSYEALAEGLDADALIQRILNHLPPPARVLQHSAEEAAGA
                     EARRA"
     gene            complement(23771..24673)
                     /locus_tag="PK518_01550"
     CDS             complement(23771..24673)
                     /locus_tag="PK518_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM47624.1"
                     /translation="MPRSVRYSPSRGARALPDAGPAAPQRATTPTPEPVRRHRRLPSP
                     QQLQWAAMALLALVLAAALWWLQPAPQPRITQKMIDAAVLHTLEHNSLPSATARAAEA
                     VAPSVVRVIGYATSKKGKEVERSVGTGVVIVDKGIILTNLHVVRGVDRIGVTFADGTE
                     SDASITGAQPENDLAVLQAHKLPDDLQAAPLRAAHNLRVGEGVVVMGFPFGIGPSASA
                     GVVSGLQREFESPEGGQELQNLIQFDAAANPGNSGGPLVTLDGEVVGIVTAILNPTPA
                     RTFIGIGFAVPIENAARAAGMTPF"
     gene            complement(24751..25122)
                     /locus_tag="PK518_01555"
     CDS             complement(24751..25122)
                     /locus_tag="PK518_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRM47625.1"
                     /translation="MSSSTDFKALTREISGNVSQLRTSQPEVMQSFNAMGKAALAPGA
                     IDAKTKELIALGIGVAARCDGCIGFHTQALARLGATRAEVHETLAIAVYMGGGPSLMY
                     AANAVAAFDQVAPEQVPPPAA"
     gene            25272..25553
                     /locus_tag="PK518_01560"
     CDS             25272..25553
                     /locus_tag="PK518_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM47626.1"
                     /translation="MQQGAAKAAALLRTVGNEQRLLVLCLLIDQGEAAVGTLLEQVQL
                     SQSALSQHLARMREEGLVTYRRDAQTLYYRIADPAVEKLVSALKDIFCG"
     gene            25611..25805
                     /locus_tag="PK518_01565"
     CDS             25611..25805
                     /locus_tag="PK518_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRM47627.1"
                     /translation="MKNVGGLDRGLRIIVGLVLIALAATGTVGWWGWLGVVPLLTGLI
                     GGCPAYSLLGVNTCPVSKKN"
     gene            25833..26666
                     /locus_tag="PK518_01570"
     CDS             25833..26666
                     /locus_tag="PK518_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRM47628.1"
                     /translation="MQRWIDTHCHLDEFAAHGGMAYADAERMRAAQAGVAHCVLPAVE
                     LANLQAVRAMAQRHGDSYALGIHPLYTPQAGEDDIQALAAALQQCRPDPRLVAVGEIG
                     LDFFVPGLDPERQQRFYRAQLQLARRFDLPVILHVRRSADQLLKALRELPVRGGIAHA
                     FNGSLQQAQAFIDLGFKLGFGGAVTYERALQLRRLATELPLYALVLETDAPDIPPHWL
                     YVTAAERAAGRAPARNSPAELPRIAAVVAALRGISGAELARATRDNACAALPRLASLL
                     G"
     gene            26738..27283
                     /locus_tag="PK518_01575"
     CDS             26738..27283
                     /locus_tag="PK518_01575"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRM47629.1"
                     /translation="MEAATSSGRLVGLPPVVGAGTRVLVLGSFPGAASLAARQYYGHA
                     RNQFWPILAALWPQHPLPDRGHYAARCDWLLARGLGLWDVYAACERQGSLDASIRNAR
                     VNDFAGLRAQAPRLAAIAHNGGESFRHAAAVRASLGQGDGAAIASLRLPSTSPANASW
                     SLDRKLQAWGETLAAFGLITN"
     gene            27280..28041
                     /locus_tag="PK518_01580"
     CDS             27280..28041
                     /locus_tag="PK518_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HRM47630.1"
                     /translation="MTRKKTATAELPEVSVSDDGEVRHLHLGTPWIQGSMRIDAPFDL
                     ELEYVQRMMAWLLFVEPTSVARRHAMQLGLGAGAITKFCRKKLRMCCTVVELNPRVEA
                     VCRAWFKLPHDGPMQRVVLADAALEIQKPEWHGTVDALAVDLYDHDAAAPVLDSADFY
                     ADCRALLTEDGCLTVNLFGRSSSYERSLDKLAEAFGDDALWAFKATREGNTVVLAQRT
                     PTRPKRAELLARAEVIQERWGLPATKWLRVFKPVA"
     gene            28065..29858
                     /locus_tag="PK518_01585"
     CDS             28065..29858
                     /locus_tag="PK518_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRM47631.1"
                     /translation="MTTATRPDHARKPVSNAGPLQWRSIVDWLRADGVISAEQAERTI
                     ARCAQAESSQHPLVRLGNLSMLRAADDKPLDVDMLTEYVAQRCGLAFLRIDPLKVDVG
                     RVADAMSASYAERHKVLPVQVSGSEVVVATAEPFVNDWVAEVERQTKRQVRRVLANPQ
                     HITRYTAEFFALAKSVREASKSGGSAGGSSFEQLVELGKSNKQLDANDQGVIRVVDWL
                     WQYAFDQRASDIHLEPRREQGVIRFRIDGVLHAVYQMPMGVLNAMVARVKLLGRMDVV
                     EKRRPQDGRIKTRNPRGDEVEMRLSTLPTAFGEKMVMRIFDPDNAVKDLDALGFTPHD
                     AQRWERLVKRPHGIILVTGPTGSGKTTTLYSTLKRVATEEVNVSTVEDPIEMIEPSFN
                     QTQVQPQLDFTFAEGLRALMRQDPDVIMVGEIRDLQTADMAVQAALTGHLVFSTLHTN
                     DAPSAVTRLMELGVPPYLINATLLGVLAQRLVRTLCRQCRQLDEAASPEALAELIRPW
                     KLNGGYRAYRPVGCVDCRMTGFRGRMGLYELLTVSEALKNQIHQSPSIDALRRQAVQD
                     GMRPLRLAGALRAAEGLTTLDEVIAATPPLE"
     gene            29991..30479
                     /locus_tag="PK518_01590"
     CDS             29991..30479
                     /locus_tag="PK518_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRM47632.1"
                     /translation="MKIKSQQDFFAGLMFLVVGGGFAWGATTYNVGTGARMGPGYFPL
                     MLGILLAILGAIIMLKALVVETVDGAPIGKWAWKQIFFIIAANLLFGVLLAGLPSLGV
                     PAMGLIVAIYGLAFVASLAGTHFHFKGVFILATILAAGSYAAFIWGLNLQFPVWPSFI
                     AG"
     gene            30496..32010
                     /locus_tag="PK518_01595"
     CDS             30496..32010
                     /locus_tag="PK518_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRM47633.1"
                     /translation="MELFDHLSMGFGVAFTFQNLIYCFVGCLLGTLIGVLPGVGPVAT
                     IAMLLPATYALPPVAALIMLAGIYYGAQYGGSTTAILVNLPGESSSVVTVIDGYQMAR
                     NGRAGPALAAAGIGSFFAGCVGTLILAAFAPPLTEVAFKFGPAEYFSLMVLGLIGAVV
                     LASGSLLKAIGMIVLGLLMGLVGTDVNSGVARYSFDIPELTDGISFTVIAMGVFGYGE
                     IIANLSKPEHEREVFAAKVTGLFPTAQDFKRMFPAMLRGTALGSALGILPGGGALLSA
                     FAAYTIEKKSRLKPGEIPFGQGNIRGVAGPESANNAGSQTSFIPLLTLGIPPNAVMAL
                     MVGAMTIHNIQPGPQVMTSNPELFWGLIASMWIGNLMLIVLNLPLIGMWIKLLTVPYH
                     WLFPSIVLFCAVGVYSTNNNTFDVWLVGAFGFVGYMFYKLGVEPAPLLLGFILGPMME
                     ENLRRALLLSRGDWSVFVTRPISAGLLAAAALLLVIVLLPAVSKRRDEAFVEED"
     gene            32101..33393
                     /locus_tag="PK518_01600"
     CDS             32101..33393
                     /locus_tag="PK518_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HRM47634.1"
                     /translation="MPLDYLPAQHPQRAQLHNEIHARPPEALAAPVAIAHIVMWADAA
                     QREASRAHLAALLRDQHQAMPDAHSTHLRANLGGFRLRWELHTEFVTWTFSVPLPADA
                     WNQRAPAAATEAVPREWLSQLPGLCLCQLQLWVLPTREYDELPQLGELLREDSLVASS
                     VAQGHGEVFTDFCVHADGSSRMVLLAGSMAPRRLGRLVQRLLEIETYRMAALLGLPVA
                     REAAAVLGRAEQELAALADAIRSADRDMEPELLDRLTRLAGQVESQYAATQARFSASS
                     AYFELVDLRIRDIAESRLGGMQTIGDFMDRRLSPARSTCEWVARRQNALSQRVSRISN
                     LLRTRVEIEQQQSSQALLATMNQRQDLQLKLQSTVEGLSVAAITYYIVGLVSYLAKGA
                     QKLGWPWSPESTAAVAIPLVAGAVWWSLRRLHQRMFKH"
     gene            33408..34238
                     /locus_tag="PK518_01605"
     CDS             33408..34238
                     /locus_tag="PK518_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM47635.1"
                     /translation="MDFGIEGKWALVCGASKGLGLGCAQALVGEGVNLVINARAPDAL
                     HQVASQLIAIGADRVSARGQNGLKPQVLAVAADITTPEGRAAVFGAAGGPGRDFDIVV
                     TNAGGPPTGDFRDWEREAWIRALDANMLTPIELMRATVDGMAARGFGRIVNITSSAVK
                     APIDILGLSNGARSGLTGFVAGAARGGIAAHGVTINNLLPGKFDTDRLAATLRAAADK
                     SGKSLDEVRQAQRAAIPARRFGSPQEFGAICAFLCSAQAGYITGQNILADGGAYPGML
                     "
     gene            34507..35439
                     /locus_tag="PK518_01610"
     CDS             34507..35439
                     /locus_tag="PK518_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRM47636.1"
                     /translation="MNQYTQWEEPFWTRELPRISGGRFKAEIVPFDRAGVPGEDMLRL
                     LQLGVVPMATVLMSGLTAQYPEYTAPDLAGLNPDMASARAAVAAFRPFLEKNLREQHG
                     VRLLAVYAYPAQVVFCKKAFSALTELAGRRVRVSSAGQADFMEALGARPVQLPFARIV
                     AQIESDSIDCAITGTLSGHGLGLHQVTSHLHAMPLNWGLALFGANQGAWEALPAELRQ
                     LLASELPKLEEAIWTQSESDTRRGMACNTGQAACAQGRKGAMALVPVTPQDERRRQEI
                     FTNTVLPRWLQRCGQRCADVWRQTLGPTLRANPP"
     gene            35459..38089
                     /locus_tag="PK518_01615"
     CDS             35459..38089
                     /locus_tag="PK518_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM47637.1"
                     /translation="MRLNPARVPVLFGLLAALLVVVLAGSALGLVWSMRTTAMRNAEG
                     LVENMAMGVEATLNRHLLSLDLLLSGVQDQLQVTHANDRSDDGRLLTMISRDNLLVGR
                     LLLLDAKGGVHAVSGSSGALQEFSLPPAFWAEITQSKAARMHISGPVQSFASAQQVLF
                     FARRLSLDGMPYWAVAEVPLAKFTEVAQGSERSGFEVLLEKTNGQRLFAVPDLPVSAG
                     QDLMAHSPLPERAWNVPARISGAPALVLGRSLLYPELRFSVSLPMALALARWQAERNA
                     VLLVVALFATVLLLAAVVAGRVFRQLSAARQNTAASKAMLDEALESMVGGFVLLDSQR
                     RVVHWNRRFVVIYPWLAPYVAPQLPFRVLLEQSARNLLPAASQAEREQWIQERQAQQR
                     EGMGSAEQLLPDGRCIQLQERHTPDGGLVITYHDVTDLRRAKEEIENLAFYDALTGLA
                     NRRLLLDRLGRAIVQAQRTGKVGALLFLDLDHFKALNDSKGHEMGDELLQQVARRLLA
                     HVREMDTVARLGGDEFVVMLRDLSASREGAAAVAQRIAEKLLQALEEPFQLGGHLYQG
                     AASIGATLFCADVDSATDLLRRADIAMYQAKARRGNALCFFDPQMQAAINERAQLESD
                     LRQALASDQLVLHYQPQCTASGDVLGAECLVRWQHPERGLVPPGQFIPVAEGSDLILG
                     IGQWVLRAACRQLAAWAHQADCARLRLSVNVSARQFRQDDFVCQVDRVLQTTGASAHL
                     LTLELTESMVLDNVQDAIEKMDQLRAKGVQFALDDFGTGYSSLSYLTRLPLHTLKIDQ
                     SFVRHLGERPADNAIVQTIIGMGRNLDLAVIAEGVETQAQKDLLAGYGCDLYQGYLLG
                     RPAPIEVLQALLAREVASAA"
     gene            complement(38078..38977)
                     /locus_tag="PK518_01620"
     CDS             complement(38078..38977)
                     /locus_tag="PK518_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM47638.1"
                     /translation="MTQRLSPGTAAMLAAAPLLWAGNAVVGRALHTLIAPSTLNFLRW
                     LLAFALLLPLAWQVLRPASGLWPHWRRYAALGMFGIGSYNALQYLALQTSTALNVTLV
                     GSGMPVWMLVVGALFFGVPVTRRQAVGALLSVTGVVLVLSRGSVGELLALRLVPGDLY
                     MVLATISWAFYSWLLVRTSEPQGLRGDWAAFLTAQMFFGLGWSGAFAGAEWSLGQGHI
                     AWGWPLAAGLAYIVIGPAILAYRFWGAGVQRAGPVAAGFFINLTPLFAALLSAAFLGE
                     APHLYHGAAFVLIVGGIVVSSRR"
     gene            39082..40068
                     /locus_tag="PK518_01625"
     CDS             39082..40068
                     /locus_tag="PK518_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRM47639.1"
                     /translation="MTLAVQIRQHGGPEELQIVDVQVGEPGPGQVRIRHHAVGLNFID
                     VYHRTGLYPLNMPAGIGMEAAGVIEAVGEGVTHLQVGDRAAYASNPPGSYSERRVMPA
                     MCVCKLPDAISFETGAAMMLKGLTAQYLLKKTRPVEGLEPGDFVLFHAAAGGVGLIAC
                     QWARALGLRLIATAGSDAKCQLALDNGAAHAINYRQEDFAARVKEITAGQGVKVVYDS
                     VGKDTWDKSLDCLRPFGLMASFGNASGPVAPFAPGMLGAKGSLYVTRQTLFTHIRTRG
                     ATQAMADELFAVVASGQVKIHIAQRYALTDVQQAHRDLEARKTTGSTLLTLP"
     gene            40068..40886
                     /locus_tag="PK518_01630"
     CDS             40068..40886
                     /locus_tag="PK518_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM47640.1"
                     /translation="MAATTAPAWVAAARARATCAPAAPRMALLLGGERVGSVDGQIFN
                     KIGLQRLLDKRYQLSIVEYEGAPAWALAADDATAALNDLALTLRAAGRCGPWRDEQLA
                     VCAADGRRLATVERGAARVLGLATQAVHLVGCTADGAAMWVQQRAHSKASNPGMWDTL
                     MGGMVSAADSLQTAVARETWEEAGLRMAQLSGLRPGGQVRFALPSDEGGGAGYMVECI
                     HWYQATVRAGATPDNQDGEVERFDCPAHGEVQARLAQGAFTPEAALVLAAFYGW"
     gene            complement(40894..42561)
                     /locus_tag="PK518_01635"
     CDS             complement(40894..42561)
                     /locus_tag="PK518_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRM47641.1"
                     /translation="MKHLLNLLAAIALLVWGIQMVRTGILRVFGANLRQLIARGVRTQ
                     LLAVFSGVGVTAVVQSSTATALIVSAFVGQGMISLTAALTVMLGADVGSALMAVVFSL
                     DLSWLSPLCIFVGVVLYVARQDGKAGRVGRVLIGLGLMLLALRLITESTLVLTQSEVV
                     RTLLMALTSDITLEVMTGAILAIISYSSLATVLLVATLAGSGGIPTEVALGLVVGANL
                     GSGLLAVLTTMRSSIQTRQVPLGNLLFRMVAATVMIPVIGLWHTHIQTLIPDKVSQVV
                     LFHLAFNIMGTLLCLPITGLVATAVGKILRQEEQPASLSRPHHLDASALSTPSLAISC
                     AAREALHLADLVETMLNGLKTVWQTDDDKLGAELRGLDDQVDSLYSAIKYYMTKISRS
                     ELDEAEGRRWTEIIGFTINMEQVGDLVERVLQDVEDKKIKKGRQFSHAGLQEINEMHD
                     HLVANLRLAMSVFLTSNVRDAQKLLEEKNRFRELELAYAATHLNRLAENTIQSIETSS
                     LHIDIISDLKRINSLLCSVAYPVLEASNAVAPLRPEAEVPSTQNDRL"
BASE COUNT         6672 a        13846 c        14948 g         7219 t
ORIGIN      
        1 ttgtggatca cgcgatcctg cgccgtgacg cctggcatgt agcgcatctt gatccaggca
       61 tgctgtgctg cccgacggcc gcatcctgct ggtgggccag ggcagtgttg tggccacaag
      121 cagcgatggt ggccaccatt tcagcttcag cagggtggcg gggcgcgcca ccttgaccga
      181 cattgcgctt gattccgagg gcgcaggctg gatcacctcg gacgccggtc tgcaacccta
      241 ccaggtcgcc gccaagggcg agagcagcaa cctgagttct gccgtcgcca agccctgatg
      301 cccaaaaagg cttgagaatc aagcccatac ccgtccgtgg gatgggggca agggcaggcg
      361 gagggcgtgg cgttgttccc agcgtgggaa agtggctgtc gtgttgcagg cagggctggt
      421 gtggccttgt tcgctttgcc atgcgccggc ctgtgcctgt atgcggcggc gcagcacccg
      481 gtcgcgccca tgctggcctt gtccctgctg gcggcggccc tggtctgggc catgtggcgt
      541 cccgtcgatc tttggtttgt gctgccagcc ttgttgccgg tagccagttt cgcgccatgg
      601 accggttggt gggtggtcga tgagtccgac ctgttggtgc tggccctgtt gggcggcgcc
      661 tatgggcgct gggcgcttga tgcctggcgt gtgcccgcgg cggtgcatgg caccacgccc
      721 gcgggcatca ggtgggcgta ttgggtgttg ccaccactgc tggtcgttgg cgtctggcgg
      781 gggctggacg acgcgcgcgg cgcggcaagc tgggtggcgc tgctggcgga tctgtgggct
      841 caggggcccc atggcgacta tgaccagccg ggaaacaccc tgcgtgtggc caagagcctg
      901 gcatggggat tgttgctcat gcccgccttg tatcgatcct cgcggggagc cgcgttgcgc
      961 ctggcgcgtg gcatgctggt ggggctgttt tttgtctgca tcgcggtgct gtgggagcgg
     1021 gggctgtatg tcgggtggct ggacttcagc gccaactatc gtacggtggc ctggttctgg
     1081 gagatgcatg tgggcggcgg cgccatcgat gcctacctgg ccatggcgct gccgtttgcc
     1141 tggtgggccg catggtctgc cccgcatggc tggcgctggt atgcggcggc ggggctggtg
     1201 ttgttggcaa cctatgcggt gctgaccgcc tatgcccgcg gcctgtatct gacggtggtc
     1261 atcacggcgg ttggcatggc ctggttggcg caccgccatc ggttgaaggc gcccgacgca
     1321 accctgtggc accgcagggc catggcgtgg ctgttggtgg ctctggtggt ggaaatcctg
     1381 gcggtttggg tgggcggagc cttcatgtcc gatcgtctgg gtcgctccaa tgccgatctg
     1441 caccagcgcc tggcccattg gaagcgcggc gtgcacatgt tgcatacgcc cggccagtgg
     1501 gtgctgggct tgggcttggg gcgtttgccg gcgcagtaca gcgcgcagaa ccccgagggt
     1561 gcgctacccg gccgggtgcg ctgggtgcgc catggcgatg ccggcaatga ggtctggcta
     1621 tatggcccgc agcgtgccga cgccaagggt ggcctggcgc tgacgcagca tgtgccactg
     1681 gaatccggcg gacattacac ggtgcgcttg cgcgcgcaca tgaatgcgcc catgtggctg
     1741 aaggttcagt tatgcgagca gtatctgctc taccaggcac gatgccagct gcgcacgcgg
     1801 caggtgtcag aagccagcaa gacgaccgat ggctggatcg tgctgccttt gctgggcccg
     1861 gccttggcat cgggcggtcg tggcacggtt tcgcgggatg gggttctttc cgtccagctg
     1921 ctgcaggcca atgccagcat tcgcatcacc gccgtggaac tcctggacaa gagcgggagg
     1981 caggttctca agaatgcgga tctggccctg gggcctcgct attggagcag tattgcctat
     2041 ggcaactttc tgccatggca tatggataac ctgttgctgg agctgctgat agagcgcggt
     2101 ctattggctg tgctgattct tgcgctggcc gtggcatggg cgctgctgca actggccaga
     2161 ggagttcggc gtggatcacc gctggccctg gttgtcggcg gctccatcat cgccggcttg
     2221 atggcgggtg ggctgataag ctttgcggaa gttccgcgtg tatctcttgt gttgtggttg
     2281 ttacctatcg tatcattctg catcacggag gatcggtagg ctcgggtgct gggatttggt
     2341 ctgagacggc gaattgttaa atttatttgc caaaccatta gaaattgttt cgccgctttg
     2401 aatttttttg cctattgatt tctaatgtgc ggttgttttt ttaatggtgg cggcgtcttg
     2461 cgaagagggt gacgatggtc aggattttta atcattattt ccgccagcga agtattttac
     2521 aggccatggt atatatggtc ttgattgtga gtactttggt tatttccatg gcctggcagg
     2581 ttggcgccga ggcgctcaat ggtgcaataa tatcttcagg cgtcattcgc ggtgggttga
     2641 tcgctgtcgg tatattgggt atgaatgctg cattgggtat tcacgaatcg cccagtggtc
     2701 tgacggtggg ccaggtgcga tcccgtgctg tattgtcctt tattttgtcg atggtaattg
     2761 ctgccggtgt tttgctgctg ctgccattgt cgccccctta tcataatgag caaactttat
     2821 tggcgttgct gatcatggta agtgccatgt tgattttgcg gttgcttggc aatagtttat
     2881 tacccgcttc ttacaagcgc cagcgagtcc tggtttatgg cactggttcg cgcgcttgtg
     2941 tggtgactga atccctgatg aaaaacgacc ccagcattga gctggtcggg cattttcgcg
     3001 gcccgcagga gaaggagcag gttgcatcgg gtttacatgt ggtgtctgcc ggcaagacgc
     3061 tcaccgaggt tgttgaggaa taccgggtca atgagatcgt ggtggcggtc aatgagcgcc
     3121 gcggcggcag catgccgctg cgcgagttgc tggactgcaa gctgcaaggt gtacaagtca
     3181 ttgatttggc gacacatttt gaaaaacttc tggggcaggt gcatctggag gcggtatccg
     3241 cagggtggct gatctttggt gatggcttca aacagggggt ggttcgctcg ctggtcaaac
     3301 gcctgctgga tgtcgttgga tcagccgtgc tgttgttgct cgcagcaccc gtgatgctgg
     3361 tgacggcggt tctggtcttt cttgaaagtg gtctgcccat tttttaccgc caggagcgcg
     3421 tgggtctgaa tggccagacc tttaatgtca tcaagtttcg cagcatgcgc caggatgcgg
     3481 aaaaagatgg aaccccccga tgggcaggtg ccggggacgc ccgggtgacg cgggttggca
     3541 aattcatccg caagacgcgg atcgatgagc tgccacagtt gctttgcgtg ctcaagggcg
     3601 atatgagtct ggtaggcccc aggcccgaac gcccgtactt tgtcgaacgc ctgacgcagg
     3661 acattcccta ttacgccctg cgccacggcg taaaaccggg cctcactggc tgggcccagg
     3721 tgcgctacca ctacggcgcc tctgtgaaag atgccgagca aaaattgcaa tatgacctgt
     3781 actacgtcaa gaaccactcg tggtttctgg acttgatcgt cttgtttgaa accgtgggtg
     3841 tggtattgac gggcaagggc gcgcaatagg caagcgctgg cttattgttc agccgtttcc
     3901 tgcggcaaca gcaaccgaaa ttcggcaccg gcgccgctgc tgctgcgcac ctgcaccagc
     3961 ccgccatggc gttgcagcac ggtctgtacc agtgccaggc ccaggcccac gccggcgatg
     4021 cctggatggc tgccttcgtg caggcgcttg aagggctgga acagatggct ttgttgctcc
     4081 ggcgcgatgc ccgggccttg gtcgcgtacg ctgatcaccc aatacggcga ctccagggtc
     4141 agggcgcatt gcacggtgct gcccggcggg ctgaatttga tggcgttgcc cacgatgttg
     4201 gtgatggcgc ggcaaatcaa ggcgcggtcg ccatggacag gggcggcttg ggggaggtgg
     4261 gtgatgtgca tctgcacctg ctgatcctgc gcgctggccc aggcgtcgtc cacggtttcc
     4321 tcgagcgcgg cggccaggtc gaagggggtg ctgtggtatt cgtaggcctg ggcgctggcc
     4381 agttgcacga agccctgggc catggccagc gacgagcgcg catggcgctc gatgcgcgcc
     4441 agcagatcct gctgcgacaa ctgctcgggg aattcacgcc gcatctccag cagcgtgagt
     4501 atggacgcgc cgggcgcgcg gatgtcgtgc gagatgaagt gcagcgcctg gtcgcgttgc
     4561 tgctgggcgc ggcgcatgtc ggtcagatcc accagggtga tgagccagat ggtgctgccc
     4621 tccagcgcaa aaggtttgca cagcatgagc atgtggcgcc cctgcgcgtc tcggccttcc
     4681 tgttgtgctg gcaactcgac cattcggctg gcgtcctgcg gcagcagggg cctgcctgaa
     4741 tggggatgga ccagctcgac cagcagctgg gcgatggcct gcccctgtgc tgcctggccg
     4801 gatggcaggt agagcagtgc ggcttcgttg gccagggtca cgcggcccct tgcgtcgcag
     4861 acaaaggtgg gcgatggcag gtgctgcagg ctgtcgctga tgaagtgatg cagcttgcgc
     4921 aaccgccttg tggcgtcttc cacggcgtgt atgcgctgtt ccagcaggtc gccgcgcagc
     4981 accaggggca ttgcctcgtc gggtaggggg gaaagccccg cgccgcgcag cgcctggatc
     5041 tccagctgca ggaaatgggc ggcggcgctg agccggcgcc agctccacag cgggtacacg
     5101 gcggcaatgc cggccagggc cggtgcggcg gcaaattgcc agcccgatag ggtcggcgcg
     5161 ccagccgctg ccaccagggt ggcgagaaac agggtggcac tggcgatcag gccggccagt
     5221 ggccccagca gcacgatggc cagcagcgtc agggcaacag gcgtgaggtt gaaggccagg
     5281 ttccaggcct cgggcgcgcg ttggacatgc cagcgcatga ggtctgcatt cagcgcgtgg
     5341 gcgatcactt ccaccccggg aatgcgttcg gcctggctca ccatgggggc ggcgaacatg
     5401 tcgcccaggc cggtggcggt ggcacccacg atgacgtatt tgtcccgcag ggcgccggct
     5461 ggcagctgcc cggtcaatac atcaatgtag gaataggtgg tgaatgccgg ttgtccttgc
     5521 gcaaagcgca ggatctgaag atcctggctt gtccagggag cattcaccgg cgcggcgttg
     5581 ccccggcatg acgccaatgc cagcccttcg gcgcacagca tcgcggtgct gaaatgcggc
     5641 cagggcgcgg acgctggccc ttcgtgttca aacaggctgc gcgccacgcc gtcttcgtcc
     5701 agctgcacct gcacatggcc gagctgggcg gccgcctggc gcagcagcgg catgggtgcg
     5761 tcggcgctgc tgcgcggccc gtcaccgcgg cgcgccacgg gcagcacggc gtgggcgttg
     5821 cgctccaggg cccgggccag cagcaggtcg tcaccggggt agtcggcgtc ttcttcgcca
     5881 aacagaatgt ccagccccag tgcgcgtggt gactgtgccg tgatctggtc cacgagctgg
     5941 gcatgcagcg cgcggcgcca gggccagcgg cctatcgagg ctatgctgcg gtcatcgacg
     6001 gccacgatga cgatgtccgg gtgtgccgca tgcgcataca ggcgcgtgcc tgcgtcctgt
     6061 accaggtagt ccatgcgccg caggccgccg gcaaggcata gccatgccac caggcccagc
     6121 atcagcagcg acagcaccag ccattcgcgg cgcaggttgc ggtggtaacc cgcctgtgca
     6181 gatagcgtca tatcacgcgg cggccccagg cctggtgcgc tggggcgggt ttcagggccg
     6241 tgccaatggg cggccgtcgg acgatgtcac cggcagaccg ccgctcgatt gcaccgccgc
     6301 ccggacatgg atcaggcgcg gcgtggagaa ctcgctctcc agcccggaag ggtcgcgtgt
     6361 ctgtatgcgc acaaagtact cgccgggtgc cagactggcg gctgtccagg cagctgtgcc
     6421 gaggcgttcg tccatcagca gcgcggcaaa gtcctggctg ctggccagct gcaggcggta
     6481 gctctggccg ctgcctccgg gccagcgcag ctgcagggcc gggccttcat tgccaacctg
     6541 cagggcggcg gcgtccgggg cgttcgggtt gttcgccacg ctgaatggct gggcgggtgc
     6601 gtaggggccc tggtcgccgc cgccgccggg cagctcgcgc acgctggccg cacgccagaa
     6661 gtagttgccg ggggccagcg cctgcacttg cgctccgcac tgctggtcgc gggtttcgtc
     6721 cagcgccggg gcggtaaagg cggcatcggt agccacctgg atgcggtagc gcacagcgcc
     6781 cagtaccggc gtacacagca gctcaccctg ggtacgcgag atggtcgctc cctggggtgg
     6841 cgcctggtac agcggcggaa cggggtgggc cttgacggtg atggtgcgct gtgccaccag
     6901 gccgggcagg ccggtgctgt ccacggcgcg cacggccagg tggtagctac cgtcgtccac
     6961 ggcgcgcatg cgcgcctggg ggccggagaa ggcaccatcg cgcaccacgg tggtgaagtc
     7021 ggcgtcgcgc gccacccgca cctggtaggc cacggcagcg gccactggtg gcagggccag
     7081 gctgaggaag tctgcgtcat gcactgccgc gggcagcgcg ctcaggtcgg gcgcgggcag
     7141 caggggctgc ggtgccccca gctcgccgtc ggccgccacg accacgccct ggccggccgg
     7201 tatcgatgcg ccctggcgcg tggcatgggg ctgcacggcc accgtgcctt cggtgacagc
     7261 cgtcagggcg cgctcatcgc ccgtcagggc caccgaaaag cgcgtgccgc gcacgctggt
     7321 ggatgccttg ggtgtgcgga tttcaaagcg gcgtgcaccg ccgctgttgg cgggcacgga
     7381 ggtctccacg ctgccgcggc gcagttccag caccgactgc gcatcgcccg cgcggccgcg
     7441 ccggcgcagc tgctgcagct gcaggtcgga gtcggcatgc acgcggatca gcgtgccgtc
     7501 ggccaggcgc acggtgacga aggagttggg cgcgacctgc acccgcgcgc cctccggcag
     7561 tggctggccg gcttgcaggg tggtggcgct ggcgttggtg ggcgcagtga ccgtggcctc
     7621 gccctgcacg aaggtcacct cggccgagcc cagtggcagc agctgcatgg ggatgtgcag
     7681 caccgaaccc ggctgcaggt ggcgcgggtt ggcgacttgg ttgtgccttt gcagctgcgg
     7741 ccacaggcgc ggcgtgccga ggtagtgcgt cgaaagtgcc tccagcgtgt cgccgcgttg
     7801 cacctggtgc tctatgtcca gtggctgggc cagcgcgccg ccatgcgcca gtgcggcgca
     7861 gagggccagg gccagaggta gtgtctggcg tgcgtagcgg tgtggcatgg gtgtggcttg
     7921 catcggcttc tccctcgtga tccaggcgtg ccggctccgt ccagggccag gccgcatggc
     7981 ttgttggccg tgcctattgt gcctggctgg cgctgccgtt agccgtttgt tcggccgtcg
     8041 gcttgacggc ctcgaagcgg tagcccacgc catacacggc ggtgatcatg tagccgttgg
     8101 ccgggcgcag atccagcagg ctgcgcaggc gcgagacatg cgtgtccagc gaacgcgaga
     8161 tgacctcggc gctatggccc cagatgactt cgcgcagatg gtcgcgcgac agcagccgcc
     8221 ccatgttctg gaacaaaaac agcgccagct cgtattcgcg cgccttcagc tccacgggct
     8281 tgccatccat ttccaggccg cgcgcctcgg ggagaaagcg gaagcggccg aactccagca
     8341 cgtcctgggc gtcgttcggg taggcgcggc gcagcagcgc acccacgcgg gccttgaatt
     8401 cggccagacg cacgggtttc accatgaagt cgtcggcgcc acagcccagg ccctgcacga
     8461 tgtcgctttc ggactggcgg tgggtgacga agagtatcgg caactcggtg tccagctgct
     8521 ggcgcaccca ggtgatgatc tcggggccct cgatgtcggg cagctgccag tcaacgatca
     8581 gcagatcgaa ggtttcgcgc cgcaaatcct tgagcagcgc cgcgcccgtg gtgtaggtgc
     8641 ggcaggcgtg gcccatggcc gtcagggcgt gggtgaaaaa ctcgagctgg agcggatcgt
     8701 cgtccagtgc cgcgatgcgc atgggactcc ttggaaatgg gggcggagtg ggcaagtata
     8761 ggaaaaggcc agaagaaccc cgcctgcatg acgctgccct gttggatggg gcatacagcc
     8821 cgtccaggtt ttttaggtgt tattggctca tagcgcttta ctgacaagcg ctataagcta
     8881 caaaaaacgt agtaaccgat ccatctcagg catcggccgc cgcggccagc gcctgcaggg
     8941 cggctgtcac ctcggcggca tccacgccgg ccaccggtgc cagttgcgcg gccagcgcct
     9001 gcagggcgtc ggcctctttc tgcaggcggg cgatggcctg gccggcatcg cgtggcgcgt
     9061 cgaacgccgt gctttgcagc agcagccggc cgtcgctggc gacgagcttg aagtagaact
     9121 tgccgtctgc ctcgcggtat tgcttgaagg ccggcagctg cgccttgcca gccttgtgcg
     9181 cgccctgcgc ctggggccgc gtggcgctca aggggcgcag gccgacggcg gcgcgcaggc
     9241 gctgcaacaa tggctgggcc agggcgcgcg cgcgctgggc gccgatctgc agggcggcct
     9301 cgaccttctc ggggtggagc atcaggtcgt catagcgtgc gcgcatcggg gcgatctcgc
     9361 ggtcgatgcg cgcaaacagc agctgcttgg cctcgcccca ggcgatgccc tcggcaaagg
     9421 cgcggcgcat ggcggcggtc tcctcgggcg tggcaaaggc ctggtagagc tggaacaggg
     9481 ccgagccctc ggtctccttg ggctcgcccg gcgcgcgcga gtcggtgacg atggagccga
     9541 tcagcttctt gagctgctcg ggcgggcaga acagcgcaat ggtgttgtcg tagctcttgc
     9601 tcatcttgcg cccgtccagg ccgagcaggg tggccacgtt gtcctcgatg gcggcctcgg
     9661 gcagcgtgaa atgctcgcca tacaggtggt tgaagctgga cgccatgtcg cgcgccatct
     9721 cgatgtgctg gatctggtcg cgccctaccg gcactttgtg ggcgttgaac gccaggatgt
     9781 ctgccgccat cagcaccggg tacatgaaca ggccggcgct cacgccgtca tcggcctcgc
     9841 gcccggcctc ggcattcttg tcctgcacgg ccttgtaggc atgggcgcgg ttgagcaggc
     9901 ccttgccggt aacgcaggtc agcagccagt gcagctcggg aatctcgggc acgtcggact
     9961 ggcggtagaa ggtcacctgt tcgggatcca ggccgcaggc cagccagctg gcggcgatct
    10021 ccagcgtcga gcggtgtatg cgctcgggct cctggcattt gatgagggcg tggtagtcgg
    10081 ccaggaagta gaagttctcg acgccgggcg tgcgcgtggc ggcgatggcc gggcgcatca
    10141 tgcccgcgta gttacccagg tgcggggtgc ccgagggcgt gatgccggtg agaaaacgcg
    10201 tggtggtcat gagattaacg gagcaaggcg gtgatcggca gcagcagcag gttgatggcg
    10261 gcgtagccca gcgacatcag cgggcgcagc cacagggtgc ccacgatgcc gccgacgacc
    10321 agggccatca cgatgaagaa gccatagggc tcgatgcgcg caaccagctg cgcctggcgc
    10381 cagggcagca ggccgacgag gatgcggccg ccgtcaagcg gcggcagcgg aaacaggttg
    10441 aaggcccaca tcaccaggtt cacggcaatg ccggcgcggg ccatctcgat aaagaagcgc
    10501 tcctgcccgc ccagggtcgc cagcagcacc agcagcagtg cccagccaat ggcctgggca
    10561 aagttggacg ccggccccgc cagcgccacc cagaccatgt cgcgcttggg gttgcgcagg
    10621 tggtggaagt tgaccggcac cggcttggca tagccaaaca gaaaggcgcc cgaggtggcg
    10681 aaatacagca ccagcggcat cagcaccgtg cccaccgggt cgatgtgctt gatggggttg
    10741 agcgtgacgc gtcccatcat ggtggcggtg ttgtcgccaa aatgccgggc ggtgtagcca
    10801 tgcgccgcct catgcactgt gatggcgaac agcacgggca gggcgtagat gagtacggtc
    10861 tggatgaggt tggtaaggtc cacgggcggc gattgtgtca gatgcctgct gagggctggc
    10921 gcgcgacgcg atccggtgca ggagcgcctc ggtgttcagc ctgaggtttg gtttgttatg
    10981 aaaaaaatag ctactcgcgc tcgctgcata agcgccaggt gctgttttgg cttcaaatgc
    11041 cctgtgacgg gctcacagcc ccagcgcctg caggctgccg cgcccctcgc gcaccacgac
    11101 ggggtcgccg ccggtgttca tcggcgtcag atccagcacc gtggtcggct ccagggggca
    11161 ggcgccggcg tcgatgatgg cgtccagctg cttttcgaag cgctcgcgga tttcctgcgg
    11221 gtcgttcagc ggctcggtct cgtcaggtgg tatcagcgtg gtggccagga gcggcgctcc
    11281 gtgcagctcc agcagcatct gcaggccctt gcggtcgggc acgcgcaggc caatggtctt
    11341 gcgctgtggg tggctgacgc gccgcggcac ctccttggtg gcgtccagga tgaaggtata
    11401 ggcgccaggc gtggccagct tcagcagtcg gtactgccgg ttgtccacat gggcgtagct
    11461 ggccagctcc gacaggtcgc ggcacaagag cgtcagatgg tgcttgtcgt tgatctggcg
    11521 caggcggcgt atgcggtcca cggcgtcctt gtcgtccagc cggcagacca gggcgtagct
    11581 ggagtcggtg ggcacggcca gaatggcgcc gccggacagc agcgcggcgg cctgcttgag
    11641 caggcgcggc tgcgggttgt cggcatggat ttcgaaatat tgggccatgg tgagatgttt
    11701 atcgggcggc gcgcagtatg cgttcatgca gcagctgcca cacgggcgtc agctggccgg
    11761 gcaggccggg cagggcgccc aggtcggtgt gcgattcgcc ggggctgtga aagtcgctgc
    11821 cgcgcgatgc ggccaggccg aactcttgcg ccatcgcggc gtaggtggcg tattcggccg
    11881 ccgtgtggct gccggtgacc acctccacgc cctggccgcc atgctgctgg aactcggaga
    11941 acagcgcata ttcctcgttc gccgtcaggc ggtagcgcgc cgggtgggcg atcaccgcca
    12001 tgccgccggc ctcgcgtatc cagcgcacgg catcgcccag ccgggcccag cggtgcggca
    12061 cgaagccggg cttgccctcg gtcaggtatt tgcggaacac ctcgcccgca tcgcggcagt
    12121 gaccggtatc caccaggtag cgggcaaagt gggtgcgcga gatcagctcc gggttgtcca
    12181 catagcacag cgcaccctcg taggcgcccc gtatgcccac gcgggccagc tgcgcgccga
    12241 tttcctgcgc gcgctcgccg cgcccatcgc gcgtggcggc caggccgcgc gccagttgcg
    12301 cgtcatcggc gtcgaacccc aggcccacga tatgtaccgt ggtatcgaca aaggtgaccg
    12361 agatctccac gcccgtgagg tagtccatgc ccagtgcctg ggccgccgcc gcggcacggt
    12421 gctggccgcg gatctcgtcg tgatcggtca gcgcccacag ctgcacgccg ttgccatggg
    12481 cgcgcgcggc cagtgcctcg ggcgtgagcg tgccgtcgga gacgacggaa tggcaatgca
    12541 ggtcggcgtt gagcaaatga gacaccaggg cattgtaggt tgcgcccccg ggccgcgcag
    12601 gcgtagtgat ttgcgcgaca aagccggaaa aaacatgtgt ttgttacctt aaattatgta
    12661 aacaatcgtg taagtggtgc cacactacgc ctgcagctga acccttgggt tcggccgtgg
    12721 aattctctcc ctccttgttt ttgtggatgt ggcgcgatgg tgcgcccctg agtgtcatga
    12781 atctcaatca tttgcccgtt gcacgcaaac tctgggccct ggtactgggc ctgatgatct
    12841 ccatgctggc gctgctggcc ggtctgttga gctattcgat gcatatcgac gacgatgcgg
    12901 cgcgcgtcgt gcacttcaac gaggagcgca tcggtctcgc actgcgctgg aagagcctga
    12961 gctccctggc ggtggatcgc gtggtcaatg ccctgtcatc gaacgacgag gaactgaccg
    13021 agcggctggt caagcagtcc aaggagggca tacaggccat cgacgcggtg cagaagcaga
    13081 tcgagacggc gctgttctcg cccgccgaca aggagcagtt ccagcgcgtg ctggcggcgc
    13141 gcaagcaggt gctggagcac aacgccgagg ccggcaagct gcgcgccaac ctcgatcaca
    13201 ccggcgccag ggccgtgctg gacggcaaga tgctgcccgc cgtggagcgc tatgtcgccg
    13261 agcaggacgc cttcgtgcaa ctgcaggagc gccagcgcga cgccgccaag cagcgcgccg
    13321 cggatcagcg ccagcgtgcc atatggctgg gcgttggcat cgccctggtg gtggtgctgc
    13381 tgggcatggt gctggccgcg ctgctggtgc gctccatcac ccagcccctg gccagggccg
    13441 tgggcctggc cgacaccatc gccgccggcg atctgacgca ggacgtgcac gacgaacgcc
    13501 gtgacgagct gggccagctc ttgcgcgccc tctcggccat gggcgcacgc ctgcgcagcg
    13561 tggtgggcga ggtgcgctcg ggcgtggaat cggtctcggc cgcctccagc cagatagcca
    13621 ccggcaacca cgacctgtcg gcacgcaccg aacagacggc ggccaacctg gaagaaaccg
    13681 cggccagcat ggaagagctc accgccaccg tcacgcaaag cgccgacacc gcgcgccagg
    13741 ccaaccagct ggcggccaac gccgcccagg cggccgagca gggcggccag gtgatggacc
    13801 aggtggtcac cagcatgcag cagatcaccg acagcagccg caagatcgcc gacatcatcg
    13861 gcgtcatcga cggcatcgcc ttccagacca acatcctggc gctgaacgca gcggtggagg
    13921 cggcacgcgc cggcgagcag ggccgcggct ttgccgtggt ggccggcgag gtgcgctctc
    13981 tggcgcagcg cagcgccgag gccgccaagg aaatcaagca gctgatcggc accagcgtgg
    14041 acaacgtgga atcgggctcg gcccaggtgg cccaggccgg ccagagcatg caggagatcg
    14101 tgcagggcgt gcgccgcgtg agcgacctga taggcgagat cacggccagc agcaccgagc
    14161 agcgcgacgg catcagccag gtgaaccagg cggtggccaa cctggaccag atgacgcagc
    14221 aaaacgccgc gctggtggaa gagtccagcg ccgccgcagc cgccatgcac gaacaggcgc
    14281 agcgcctggc gcaggtggtg gcggtgttca acgtcggcag cggcgcggcc gtggcggcac
    14341 gcccggcggc aaaggccgcc atgcccgcgc cgcaggccaa ggccggcgca gccagcgccc
    14401 gcccgcagat cgccaagccc gcgcccaagc cggtggccgc agccccggca cgcatcgcca
    14461 gcgccaaggc gccggtggcc gcgggtggtg atgatgactg ggagagtttt taaaaaacca
    14521 tagcttgctg cgcatacggg gcaagcgcca aaggcctttt tggccttgaa tcatgacaag
    14581 ggggcggcgg tttttataat cgccgcccca ttttttgcat tttttatgct gtcaactacc
    14641 cgctccagcg ccgcttcgcg ccccctgccg tccatgattt ttgccagccg ctggctgcaa
    14701 ttgcccctgt atctgggcct gattgcggcg caggcggtgt atgtgtttca tttctgggtg
    14761 gagctgatcc acctgctcga agccgctctg ggcgacgaac aggcgctgca ggccctggtg
    14821 accagcattg gctacaagac cgatgcaccg ctgaccagcc tgaacgagac cgtcatcatg
    14881 ctggtggtgc tggccctgat cgacgtggtg atgatctcca acctgctgat catggtcatc
    14941 gtcggcggct acgagacctt tgtcagccgc ctgcacctgg agggccaccc ggatcagccc
    15001 gagtggctca gccatgtgaa cgcctcggtg ctcaaggtca agctctccac ggccatcatc
    15061 ggcatcagct ccatccacct gctgaaaacc ttcatcaacg ccgccaacta tgacgagaag
    15121 gtgctgatgt ggcagtccat catccacatc gccttcctgc tcagcgcgct ggcgattgcg
    15181 ctgaccgacc gcctgctgag ccacaaccac ggcggcaacg cgcattgatc cccggcaagc
    15241 atgcatggct gacgtaaaaa aaggcgcaga ccatgccgcg ccttcttcta cctgccatgc
    15301 gagcctacgg ctgaccgtcg gccccgtacc tgggcgagcg cgggccgtgc agcaggccgc
    15361 cggggcggcc ggcatagagc aggcggccat tggcgatttc ggcgatctgc acgctgcggt
    15421 cggacagtga atcgacgatc tggtcgatga gagctgaaat caacataccc aatgggttgc
    15481 cgcttgtgct cttggtctcc gcgctggagg cagtggcttc gccgctccac agctggtcac
    15541 ccgtgcgcag atccaccagc cgggcggtga gtgtcacctt ggtctcgctt ttgagtacgg
    15601 catagaccga gccgtattgg cgcacgtcca ggtacagcgc ggcatcggcg ccaaagatct
    15661 cgcgcagctt ggccaccggc agctcctgcg catcctgcgc ggagatgatg ccgttgtgcc
    15721 ggaaggtctc ctcggtcacg gccaccggca gcacgtagta gccggactcg gccagcggcc
    15781 gctttgctgc aggtgggtca ccaaaatggt ctgtgcctgc ccgcccaggc gatcgacgcc
    15841 gtcggagaag atgccgccct ggaagttgga gcgattggcg acctcgccta tcgaaatggg
    15901 gctgcgcatg ccctggtagg cgcgcgtgga tgccgtgggc tttggggtgt cgagggcgcg
    15961 cgatttgatg caattttgtg ggtatttgac ctgatcaggc gggcagctcc accccctcga
    16021 ccagaaactg cacccgtcgc tcgccgcgcc actcgttcac gtccaggcgg aaggccagca
    16081 gcgcgcgctc gggcagctgc tcggtgtggc cgaaccagat ggcgtccatg ggctggccct
    16141 ggtgcagcag cttgagctgc aggtggttct tcgcctcgcc caccaggcgc tggctgagca
    16201 cctgcacctg ctcgctgaag gtgggcgggg cgaagccctg gccccagacc tgcagctgca
    16261 gctgatccac cagctcggcg cggcagtatt cgggcgccag ggggccgtcg gtctccagcc
    16321 ggcgcgtgag ggtggcggcg tccagccatt cctgggccac ctgggcgaag gcctgctcga
    16381 aggtctcgaa atgctcctcg gccacggtgc agccggcggc catggcgtgg ccgccgaact
    16441 tcaggatcac gcccggatgg cgcttggcca ccagatccag cgcgtcgcgc aggtgaaagc
    16501 cctggatgga gcggcccgaa cccttgagct catgctcctt gcccggtgcg ctgctggcgg
    16561 caaagacgaa ggtcgggcgg tgcagccggt ccttgaggcg cgaggcgacg atgcccacca
    16621 cgccctcgtg aaaatccggg tcgaagacgc tgatggccgg cggcggctcc tcaccctcgt
    16681 caaatagccc ctcggccagc tgcatggcct gctcgcgcat gccgccctcg atttcgcggc
    16741 gctcgcggtt gatggcgtcc agctggcggg ccagctccgc ggcccgcgcg gcgtcgtcgg
    16801 tcagcaggca ttcgatgccc agcgtcatgt cggccaggcg gccggcggcg ttgatgcgcg
    16861 ggcccagtgc aaagccaaag tcgaaggtgg tggcggccgg cgccttgcgg cccgcggcgt
    16921 cgaacagcgc ctgtatgccc ggctgcatct gcccggcccg tatgcgtttg aggccctggg
    16981 ccaccaggcg gcggttgttg ttatccaggc gcaccacatc ggccaccgtg cccagggcca
    17041 ccaggggcaa gagcggctcc agccgcggct ggctggtctg gtcgaagcgg ccgcgtgcgc
    17101 gcagctcggc gcgcagctgc agcagcaggt agaacatcac gcccacgccg gccatggatt
    17161 tgctctcgaa ggtgcagccg ggctggttcg ggttgacgat ggcgtcggcc gcaggcagcg
    17221 cggggccggg caggtggtgg tcggtgacga cgaccgagag gcccagcgcc ttggcctcac
    17281 ccacgccctc gacgctggcg atgccgttgt ccacggtgat cagcatgtcg gcgccgcgct
    17341 cggccacgcg cctggcgatc gaggcggtga ggccgtagcc gtccaccacg cggtcgggta
    17401 ccagatactg gacatgcctg gcgcccagca gcgccaagcc gcgcacgccc acggcgcagg
    17461 cggtggcacc gtcgcagtcg tagtcggcga cgatgaccag gcgcttgtcc tgggcgatgg
    17521 cgtcggccag cagcgtggcg gccgcgccta tgcccttgag gccgccgggc ggcagcaggc
    17581 gcgccagggc atcgtccagc tcgtcctggg cgcgaatgcc gcgcgccgcg tacaggcgcg
    17641 ccagcagcgg gtgcacgccg gcctgctcca gcgcccagac agcgcgggga gggatgtctc
    17701 tgctaattat tttcatagct gctcgcgcac gtcagcaaag cgccgaggcc ggaaaaggct
    17761 ttgaattctc tgccccaggc cacgcggcgc ggtgtgccag gtcatggcgc tgcgctcgcc
    17821 gcacagcgtc aggcgggcgc tgccgccacg ccccacatgc gcggccagct gggccaccgg
    17881 gccggcatcc agctcggccc aggcccggct ccaggcgccc cagtcctcgt gcagggcggc
    17941 ctgcagcagg ccgttgggca gctggggcgc gtgcctggcc accgggggct ggggcagggc
    18001 gcccgcgcca tgcacccaga aggcgttgac ggggggcaat cgtcgggcgg cgcgtgcgtc
    18061 gttgaacggg tgggtgtaga gcagcatctg catctcgctg tgcaggcggt gcaggttacg
    18121 ggcctgtagc ttgtcgggta gccagctgct cacgtcgcgc tgcagtacgc ggtcgagcga
    18181 ggccgtggcc aggccgtcga acgctgcgcc atgggccagc cagcgcgtgg gctggtcgta
    18241 gaccagggtg atgccgtcct cggcaaacca gggggcgagg atggccagca ggctgcgcga
    18301 gtcgtcctcg gtcagctgca gttgcgccgg gtcggccagc agcacatggt tggcgtcggc
    18361 ctgccattgg cagggggtga tgaaggccca ggccaggcca tggtcagtgg gcatatgcgc
    18421 cggcgtctgc agccggtgcc aggccgccca gggcgtggtg ccggcggggc cggcggcaag
    18481 gcccaggtgg cgcgccaggg cgcgttcgtg cggggggctg aagtcggtct cgttgccggc
    18541 gtccaggccg gccgggctca ggcgcgccag cagccggtcg agctggggca gggccaggtt
    18601 tttcattgcc tgctggcagc cctgggtggg gctggtggcg agggcgacga tcagatgggg
    18661 catgggaggc agggcagaca tcggtgggct attgtccggg atgccacaat cgcgggctct
    18721 gtgcggccag tgtttttctg tggccttgca ctctttacac catgcaaatt ccctacgaac
    18781 tggccctggg ctggcgctac acccgggccg gccgcgccac gcggcgcaac ggcttcatct
    18841 ccttcatctc gggcgtgtcc atgctgggca ttgccctggg cgtggcggcg ttgatcatcg
    18901 tgctgtcggt gatgaacggc tttcagaagg aggtgcgcga ccgcatgctc ggcgtggtct
    18961 cgcatatcga gatcttcgcg cccggcggcg cggccctgcc cgacatgcag cgcacgctgg
    19021 aggaggcgcg cgccaacccg aacgtgattg gcgccgcgcc cttcgtcgcc gcccaggggc
    19081 tgctggcgcg cggcgaggac atgaagggcg tgctggtgcg cggcatagac ccggcgcgcg
    19141 agcccgaggt gacggatctg gcggccgaga acgtggctgg cctcaaggcc ctggtgccgg
    19201 gcgagttccg cgtgttcctg ggcggagagc tggcacgcca gctgggcgtg caggtgggcg
    19261 acacggtgac gctgatcgcg ccctccggcc aggtcacgcc ggcgggcgtg ctgccgcgcc
    19321 tcaagcagat gacggtggcg ggactgttca actcgggcca ctacgagtac gactctgccc
    19381 tggtgctgat ggcgcaccag gacgccgagc gcatcttccg cctggagggg ccgaccggcg
    19441 tgcgcctcaa attgaaggat ctgcaccagg cgcccgaggt ggcgcgccag ctggcgcgaa
    19501 gcctgtcggg cgacctgctg atccgcgact ggacgcagca gaacaagacc tggtttgccg
    19561 ccgtgcagct ggagaaacgc atgatgttca tcatcctcac gctgatcgtg gccgtggccg
    19621 ccttcaacct ggtgtccacc ctggtcatga ccgtcaccga caagcgcgcc gacatcgcca
    19681 tattgcgcac gctgggcgcc agcccgcgca gcatcatggg catcttcgtg gtgcagggcg
    19741 ccatggtggg cgtggtgggc acgctggccg ggctgctgct gggcctcgcc attgccttca
    19801 acatcgacgt catcgtgccg gccatagagc gtgccttgca cacccgcttt ttgccgcagg
    19861 acatctacct gatcagccgc atgcccagcg atccgcagag cagcgacatc gtgccgatcg
    19921 ccgtcatatc gctgatcctg gccttcgtcg ccaccctgta cccgagctgg cgcgccagcc
    19981 gcgtgaaccc ggcggaggcg ctgcgctatg agtgaccacc agatcgtgct gcaggctgaa
    20041 ggcctgacca agcgcttcac cgaggggcgg ctcgacgtca gcgtgctgca gggcgttgac
    20101 ctgcaggtgc gcgccggcga gaccctggcc atcgtcggcg catccggctc gggcaagagc
    20161 acgctcttgc acctgctggg cgggttggac gcgccgaccg cgggcagcgt caccctcaag
    20221 ggccaggcgc tgggcgcgct caaacccggc gaacaggggc ggctgcgcaa cgcgcacctg
    20281 ggtttcatct accagtttca tcacctgctg cccgagttca gcgcccagga caacgtggcc
    20341 atgccgctgc gcatacgccg cgcgccctat gcgcactgcc tcgagcaggc gcggcagatg
    20401 ctggccgccg tgggccttgc tgagcgcgtg caccaccgcc cggcggaact gtcggggggc
    20461 gagcgccagc gcgtggccat tgcccgggct ctcgtcacgc gcccggcctg cgtgctggcc
    20521 gacgagccca ccggcaacct ggaccgcgcc acggccgacg gcgtgttcca gctgatgctg
    20581 caactggcgc gcgagcaggg cacggccttt gtgctggtca cgcatgacga gcagctggcc
    20641 gcgcgttgcg accgcgtgct gcggctggtg tccggggtgc tggggtgata tgaaaagaat
    20701 agctgttcac gcccatgtac cgggcgctac agcgataaaa cactataaga cccggccaaa
    20761 ccaccacacc gacagcgcgc cggccagcag catcagcgcg gcgccggcca gtgccatcag
    20821 ggccgtcacc tcggtttcct tggtttccac gctgatgcgc gagctcagag tctcgtagac
    20881 cttgcgcaga tcctgcgccg tggcggcgtg aaagtactcg gcgttggtgc gcagggccac
    20941 ggccttgagc gtgtcctcgt ccaggcgcac acgcatggac cagccctcaa agccgatcac
    21001 ctcgccctgc accgtgccca cgcccacggt gtagacgcgc acgccgcggt cggcggccca
    21061 ttgggcggcc tccagcggat ccacgccggt ggtgcgctgg ccatcggtga gcatgatgat
    21121 ggcggccgag cggtaggagc ccggcgccac cggggtgaag gttttctgtt cgggctcggg
    21181 gcgcgtgacc tggtccatcg gcagcacgcg catggcctgg cgcccgccca gggcggcgat
    21241 gtcgatgccg gcatcgggaa acagcgtggc cagcgccacc atgatgccgt tgcccgtggc
    21301 cgtgccgcgc tgcagctgaa agctgtctat ggccttgagc aggtcgtcgt ggctttgcgt
    21361 gggcagctgc gccagctggg ccgtgccggc aaaggccacc acacccacgc gcacatggcg
    21421 cggcagctcc ttgatgaaag ccttggcggc gttctgtgcg gccgtcagac ggtcgggctc
    21481 gacatcggtg gcgcgcatgc tgcccgagac atccatggcc aggatgatgg tctgctgctc
    21541 ggacggcagc ttcagcaccg ccagcggccg cgccgccgcc agcagcagtg ccgccaggcc
    21601 cagcaggaac aacagcggcg gcagatggcg gcgccattgc gcgcggctgc ccagcgcctg
    21661 gcgcaccagg gccaggccgg ggtaggcaag cgcctgtacc ttgcgccggc gcagcagcca
    21721 caggtacagc agcaccagca gcggcaggat gagcagcagc cacagcaggg agggccagag
    21781 aaacaccatc gtccgctcct tgggttcagg ctctcgcggc caggtgctgg cggtgtccgc
    21841 gcagctgcat gaagcgcagc agcgcgctca gcaaatcctc gtcggtggac agctccagcg
    21901 tgtccacccc ggcgcgcgcg aggctggcgc gcagctcggc ttcgcgctgc gcggcgaggc
    21961 gcgcgaagcg gtggcgaaag ccgcgttcgt gcgtgtccac ctgcagctgc tcgccggtct
    22021 cggggtcgcg cagcagcagc aggccgatgt cgggcagttc cagctccagc gggtcgagca
    22081 ggcgcacggc gaccacgtca tggcgccggg ccaggcgcgc cagcggcttg tcccagcccg
    22141 gctggctcag gaagtcggat atcacgaaca ccgcgctgcg ccggcgcagc aggccctggg
    22201 cctggttcag cagcagtgcc agatcggtgg tgccgggcgg cgttgccggg tcggccgcac
    22261 cctggccgcc caccagcagg tgcagcagct gcagcacctg gcgccgcccg ccgcccgcgg
    22321 gtagcacccg gcccatggcg gcatggccgt cgtgcagcac ggcgcccacg cggttgccgt
    22381 ggcgctggat caggcgcgcc agcacgccga cgaagcctgc cagcatgtcc cgtttggcct
    22441 ggcccggcgg gccgaagtcc accgagggac tcaagtccag cagaaaccag gccgtgatgt
    22501 cgcggtcttc ggtgtacacg cgcacatggg gtgtcgccag gcgggcggtg acgttccagt
    22561 cgatatggcg cacgtcgtcg tgcggctggt actcgcgcag gtcggccagg tcgatgccgg
    22621 cgccgcgcag gagcgtgcgc gcgccgcctt gcagcaggcc gtccaggcgg cgcagcacct
    22681 tccattccag gcgctgcagc aggcgctcgg cgggcggtgc ggcgtggcgg gcttcaggca
    22741 cggcgggcct cggcgccggc cgcctcttcg gccgaatgct gcagcacgcg cgcgggcggc
    22801 ggcaggtggt tcaggatgcg ctggatcaac gcgtcggcat ccaggccttc ggccagcgcc
    22861 tcgtagctga gcgagatgcg gtgacgcagc acgtcaggca ccaggtccag caggtcctcg
    22921 ggcaaggcgt aggcgcgtgc gcgcagcatg gccagggcct gcgcgccctc ggccagggca
    22981 atcgtggcgc gcgggctggc gccgtaggca atgtggccgg ccaggtccgg caggccatgc
    23041 tggcgcggcg cgcgcgtggc ggccaccagg cgcacggcgt actggatgag cgcggggtct
    23101 acatacacct tgcggcattc cttctgcagg gcggccagct gctcggtgct ggccacggcg
    23161 gccacctgct cgggcggcgc cagggcgcgc tgggcgatga cgaattcttc ctcttcgctg
    23221 gggtagtcga tcagcacctt gagcatgaag cggtccacct gggcctcggg cagctggtag
    23281 gtgccctcgg tctctatcgg gttttgcgtg gccatgacca cgaagggctc gggcacgggg
    23341 tgggtctggc cggcaatggt cacctggcgc tcctgcatca cctccagcag cgcgctctgc
    23401 accttggccg gcgcgcggtt gatctcgtcg gccagcagca ggtgggtgaa caccgggccc
    23461 agcgcggtgc tgaactcgcc cgtgcgctgg ttgtacatgc gcgtgcctat caggtcggcg
    23521 ggcaccaggt cgggcgtgaa ctggatgcgc ttgaactgcc cctggatgca gttggcgagc
    23581 gtcttcacgg tgagcgtctt ggccaggccg ggcacgccct ccaccagcag atggccctgg
    23641 gccagcatgg ccaccatgac gcgctccaaa aagcgatcct ggccgacgac gacgcgcttg
    23701 acctggtaga ggatctgctc catcagcgtg gccgtggtgt gcgtggtctg catgctgtgc
    23761 ctcctgggtt tcagaacggt gtcatgccgg cggcccgggc ggcgttctca atcggcacgg
    23821 caaagcctat gcctatgaag gtgcgcgccg gcgtggggtt gaggatggcg gtgactatgc
    23881 ccaccacctc gccgtccagc gtgaccagcg gcccgcccga gttgccgggg ttggccgcgg
    23941 cgtcgaactg gatcaggttc tgcagctcct gcccgccctc gggggactcg aattcgcgct
    24001 gcaggcccga gaccacgccg gccgacgccg acgggcctat gccgaaggga aagcccatca
    24061 ccaccacgcc ctcgcccacg cgcaggttgt gcgcggcgcg cagcggcgcg gcctgtaggt
    24121 cgtcgggcag cttgtgcgcc tgcagcacgg ccaggtcgtt ctcgggctgg gcgccggtga
    24181 tgctggcgtc agactcggtg ccgtcggcaa aggtcacgcc gatgcgatcg acgccgcgca
    24241 ccacatgcag attcgtcagg atgatgccct tgtccacgat caccacgccc gtgcccacgc
    24301 tgcgctccac ctccttgccc ttcttgctgg tggcatagcc gatcacgcgc accaccgagg
    24361 gcgccacggc ctcggccgcg cgcgccgtgg ccgagggcag gctgttgtgc tccagcgtgt
    24421 gcagcacggc ggcatcgatc attttttgcg tgatgcgcgg ctgcggcgcc ggctgcagcc
    24481 accacagggc cgcggccagc accagggcca gcagcgccat ggcggcccat tgcagttgtt
    24541 ggggcgaggg caggcgccgg tgccgcctga cgggctcagg ggtgggtgtg gtggcacgct
    24601 ggggtgcagc cggcccggcg tccggcaggg cccgggcgcc gcgggaaggg ctgtagcgta
    24661 cggaccgtgg catgcgggca cctccgtgac aggctgtgga cgctcacggt agcacggaaa
    24721 gcgcccgcgc gcatgcccgg gcggggccgc tcaggcggcg gggggcggca cctgctcggg
    24781 cgccacctgg tcgaaggcgg ccaccgcatt ggcggcatac atcagcgacg gcccgccgcc
    24841 catgtagacg gcgatggcca gggtctcgtg cacctcggcg cgcgtcgcgc ccaggcgcgc
    24901 cagggcctgg gtgtgaaagc ctatgcagcc gtcgcagcgc gcggccacgc caatgcccag
    24961 ggcgatcaac tccttggtct tggcgtcgat ggcgccgggg gccagggcgg ccttgcccat
    25021 ggcgttgaag ctttgcatca cctcgggctg gctggtgcgc aactggctca cgttgccgga
    25081 gatttcgcgc gtgagggcct tgaagtcggt ggaagaggac atgagaaaaa ctcccgtggt
    25141 tgaggtggat ggggaagcag atgccaagcc gatattgaca tatttattag aagtatctaa
    25201 attagtcggc ggtaaagtcg cacctttgcc caaaggagtg ccaagtgacc accgccatcg
    25261 accgcgagtt tatgcagcaa ggagcagcca aggcggcagc gctgctgcgc accgtgggca
    25321 acgagcaacg gctgctggtg ctgtgcctgc tcatcgacca gggcgaggcc gcggtgggca
    25381 ccctgctcga gcaggtgcag ctgagccaat cggcgctgtc gcagcacctg gcgcgcatgc
    25441 gcgaggaggg gctggtgacc taccggcgcg acgcgcagac gctgtactac cgcatcgccg
    25501 atccggccgt ggaaaagctc gtgtcggcgc tgaaggatat tttttgtggc tgaacgctgc
    25561 ggctggcgcg gcgcgcggca cgggtttttt catcaaccag gagaaaagtc atgaaaaacg
    25621 tcggaggtct ggaccgcggg ctgcgcatca tcgtcgggct ggtgctgatc gcgctggccg
    25681 ccacgggcac ggtgggctgg tggggctggc tgggcgtggt gccgctgctt accgggctga
    25741 ttggcggctg cccggcctat tcgctgctgg gcgtgaatac ctgccctgtc agcaaaaaga
    25801 attgaccctt gcgggtggca tgatccagcc gtatgcagcg ctggatagat acccattgcc
    25861 acctcgatga attcgcggcc catggcggca tggcctatgc cgacgccgag cgcatgcgcg
    25921 cagcgcaggc cggcgtggcg cattgcgtgc tgccggccgt ggaactggcc aatctgcagg
    25981 ccgtgcgcgc catggcccag cgccatggcg acagctatgc gctgggcata cacccgctct
    26041 acaccccaca ggccggcgag gacgacatcc aggccctggc tgccgcgctg cagcaatgcc
    26101 ggccagaccc gcggctggtg gccgtgggcg agatcgggct ggatttcttc gtgcccggcc
    26161 tcgacccgga gcgccagcag cgcttctacc gcgcccagct gcagctggcc aggcgctttg
    26221 atctgccggt gatactgcat gtacggcgct cggccgacca attgctgaaa gccctgcgcg
    26281 agctgcccgt gcgcggcggc atcgcccatg ccttcaacgg cagcctgcag caggcccagg
    26341 ccttcatcga cctgggcttc aagctcggct ttggtggcgc cgtgacctac gaacgcgcgc
    26401 tgcagctgcg ccgcctggcg accgagctgc cgctctatgc cctggtgctg gagaccgacg
    26461 cgcccgacat tcccccgcac tggctctacg tgacggccgc cgagcgcgcc gccggccggg
    26521 cgccggcgcg caacagcccg gccgagctgc cgcgcatcgc cgccgtggtg gccgcgctgc
    26581 gcggtatttc aggggccgag ctggcccgcg ccacgcggga caatgcctgc gccgccctgc
    26641 cgcggctggc gtccctgctg gggtaatatt caagcatttt tggcctctag cgcttatata
    26701 tcaagcgcaa gcagctatta aaaatatagt aaattcaatg gaagccgcca cgtcctctgg
    26761 ccgccttgtc ggcctgcccc cggtggtcgg cgcgggcacg cgcgtgctgg tgctgggcag
    26821 cttccctggc gcggcctcgc tcgcggcgcg gcagtactac ggccatgcgc gcaaccagtt
    26881 ctggcccatt ctggcggccc tgtggccgca gcacccgctg cccgatcgcg gccattacgc
    26941 cgcgcgctgc gactggctgc tggcgcgcgg cctgggcctg tgggacgtgt acgccgcctg
    27001 cgaacgccag ggcagcctgg atgccagcat ccgcaatgcc cgggtgaacg actttgccgg
    27061 cctgcgggcg caggcgcccc ggctggccgc catcgcccac aatggcgggg agagcttccg
    27121 ccatgccgcc gccgtgcgcg ccagtctggg gcagggggac ggcgcggcca tcgcctcgct
    27181 gcgcctgccg tccaccagcc cggcgaatgc atcgtggagc cttgacagaa aactgcaggc
    27241 ctggggcgag accctggccg cctttggatt gatcacgaat tgactcgcaa gaaaaccgcc
    27301 accgccgaac tgcccgaggt cagcgtctcc gacgatggcg aggtgcgcca tctgcacctg
    27361 ggcacgccct ggatccaggg ctccatgcgc atagatgcgc cctttgacct cgaactggaa
    27421 tacgtgcagc gcatgatggc ctggctgctg tttgtcgagc ccaccagcgt ggcccggcgc
    27481 cacgccatgc agctgggcct gggggcgggc gccatcacca agttctgccg caagaagctg
    27541 cgcatgtgct gcaccgtggt cgagctgaac ccgcgcgtcg aggccgtctg ccgtgcctgg
    27601 ttcaagctgc cgcacgatgg ccccatgcag cgcgtggtgc tggccgatgc ggccctggag
    27661 atccagaagc cggaatggca cggcacggtg gatgcgctag cggtcgatct gtacgaccat
    27721 gacgcggctg cccccgtgct ggatagcgca gacttttacg ccgactgccg cgccctgctg
    27781 acagaggacg gctgcctgac ggtgaacctg ttcggccgct cctccagcta cgagcgcagc
    27841 ctggacaagc tcgcagaggc ctttggcgac gatgccctgt gggccttcaa ggccacgcgc
    27901 gagggcaaca ccgtggtgct ggcccagcgc acgcccacgc ggcccaagcg cgccgagctg
    27961 ctggcgcgcg ccgaggtgat ccaggagcgc tggggcctgc cggccacgaa gtggctgcgg
    28021 gtgttcaagc ccgtggcctg acctgaccct tatgctgtga tgccatgacc accgccacac
    28081 gcccagacca cgcccgcaag cctgtcagca acgcgggccc gctgcagtgg cgcagcatcg
    28141 tcgactggct gcgcgccgac ggcgtgatca gcgccgagca ggccgagcgc accattgccc
    28201 gctgcgccca ggccgagagc agccagcacc cgctggtgcg cctgggcaac ctgagcatgc
    28261 tgcgcgccgc ggacgacaaa ccgctggacg tggacatgct caccgagtat gtcgcccagc
    28321 gctgcggcct ggcctttctg cgcattgatc cgctcaaggt ggacgtgggc cgcgtcgccg
    28381 acgccatgag cgccagctat gccgagcgcc acaaggtgct gccggtgcag gtgagcggca
    28441 gcgaggtggt cgtggccacg gccgaaccct tcgtcaatga ctgggtggcc gaggtggagc
    28501 gccagaccaa gcgtcaggtg cgccgcgtgc tggccaatcc acagcacatc acgcgctaca
    28561 cggccgagtt cttcgcgctg gccaagtcgg tgcgcgaggc cagcaaatcc ggcggcagcg
    28621 ccggcggcag cagcttcgag cagctggtcg agctgggcaa gagcaacaag caactcgacg
    28681 ccaacgacca gggcgtgatc cgtgtggtgg actggctctg gcaatacgcc ttcgaccagc
    28741 gcgccagcga catccacctg gagccgcggc gcgagcaggg cgtgatccgt tttcgcatcg
    28801 acggcgtgct gcacgccgtc taccagatgc ccatgggcgt gctcaacgcc atggtggcgc
    28861 gcgtcaagct gctcgggcgc atggacgtgg tggaaaagcg ccgcccgcag gacggccgca
    28921 tcaagacgcg caacccgcgc ggcgacgagg tggagatgcg cctctcgacc ctgcccacgg
    28981 ccttcggcga gaagatggtg atgcgcatct tcgacccgga caacgcggtg aaagacctgg
    29041 acgcgctcgg cttcacgcca cacgacgcgc agcgctggga gcggctcgtc aagcgtccgc
    29101 acggcatcat cctcgtcacc gggcccacgg gatcgggcaa gaccaccacg ctgtactcga
    29161 cgctcaagcg cgtggccacc gaggaagtca acgtgagcac agtggaggac ccgatcgaga
    29221 tgatcgagcc cagcttcaac cagacccagg tgcagccgca gctcgatttc acctttgccg
    29281 agggcctgcg cgccctgatg cgccaggacc cggacgtgat catggtgggc gaaatccgcg
    29341 acctgcagac cgccgacatg gcggtgcagg ccgccctcac cggccacctg gtgttttcca
    29401 cgctgcacac caacgacgcg cccagtgccg tcacgcgcct gatggagctg ggcgttccgc
    29461 cctacctcat caacgccact ctgttgggtg tgctggccca gcgcctggtc cgcacgctgt
    29521 gccggcagtg ccgccagctc gacgaggccg ccagccccga ggcgctggcc gagttgattc
    29581 gcccctggaa gctcaatggt ggctatcgcg cctacaggcc cgtgggctgc gtggactgcc
    29641 gcatgaccgg ctttcgcggc cgcatggggc tgtacgagct gctcacggtg agcgaggcgc
    29701 tgaagaacca gatccaccag tcgccgtcca tcgacgcgct gcgccgccag gcggtgcagg
    29761 acggcatgcg cccgctgcgc cttgccggcg cgctgcgcgc cgccgagggc ctgaccacgc
    29821 tggacgaggt catcgccgcc acgccgccgc tggagtgagc cgggcgcggc tggccgcagg
    29881 cgccgcccca tgtcagggaa agcttgtatt tctcggtagg tggaatccac atcaaggaat
    29941 gcgggccacg ctctcacaat cgctagtggt attcctatct ggagacattc gtgaaaatca
    30001 aaagccaaca agatttcttt gccggcctga tgttcctggt ggtgggtggt ggttttgcct
    30061 ggggcgccac cacctacaac gtgggcacgg gcgcccgcat ggggccgggc tactttcctt
    30121 tgatgctggg cattttgctg gccatcctcg gcgccatcat catgctcaag gccctggtcg
    30181 tggaaacggt cgatggcgcc ccaatcggca aatgggcctg gaagcagatc ttcttcatca
    30241 ttgccgccaa cctgctgttc ggcgtgctgc tggccggcct gcccagcctg ggcgtgccgg
    30301 ccatgggcct gatcgtcgcc atctatggcc tggcctttgt ggccagcctg gcgggtaccc
    30361 atttccactt caagggggtc ttcatcctgg ccaccattct tgccgcgggc agctacgcgg
    30421 cgttcatctg ggggctgaac ctgcagttcc ccgtctggcc cagcttcatc gcaggttgag
    30481 agggagatct agatcatgga attgtttgac cacctctcga tgggttttgg cgtcgcgttc
    30541 acgttccaga acctgattta ctgctttgtc ggctgtctgc tgggcaccct gatcggcgtg
    30601 ctgcccggcg tgggccccgt ggccaccatc gccatgctgc tgcccgccac ctatgcgctg
    30661 ccgcccgtgg cggcgctgat catgctggcc ggcatttact acggtgcgca gtatggcggc
    30721 tccactaccg ccattttggt gaacctgccg ggcgagtcgt cctcggtcgt cacggtgatc
    30781 gacggctacc agatggcgcg caatggccgt gcgggcccgg ctctggcggc ggcgggcata
    30841 ggctcgttct tcgccggctg cgtgggcacg ctgatcctcg cggcctttgc cccgccactg
    30901 accgaggtgg ccttcaagtt cggcccggcg gaatacttct cgctgatggt gctgggcctg
    30961 atcggcgccg tggtgctggc ctcgggctcg ttgctcaagg ccatcggcat gatcgtgctc
    31021 ggcctgctga tgggcctggt cggcaccgac gtgaactcgg gcgtggcgcg ctacagcttc
    31081 gacatccccg agctgaccga cggcatcagc ttcacggtga tcgccatggg cgtgttcggc
    31141 tacggcgaaa tcatcgccaa cctgtccaag cccgagcacg agcgtgaggt gttcgccgcc
    31201 aaggtgacgg gcctgttccc cacggcgcag gacttcaagc gcatgttccc cgccatgctg
    31261 cgcggcacgg ccctgggttc ggccctgggc attctgcccg ggggcggcgc gctgctgtcg
    31321 gcctttgccg cctacaccat cgagaagaag tccaggctca agcccggtga gatacctttc
    31381 ggccagggca acatccgcgg cgtggccggc cccgagtcgg ccaacaacgc gggctcgcag
    31441 acctccttca ttccgctgct gacgctgggc attccgccca acgccgtgat ggcgctgatg
    31501 gtgggcgcca tgaccatcca caacatccag cccggcccgc aggtgatgac cagcaacccc
    31561 gagctgttct ggggcctgat tgcctcgatg tggatcggca acttgatgct gatcgtgctc
    31621 aacctgccgc tgatcggcat gtggatcaag ctgctcaccg tgccctacca ctggctgttc
    31681 ccctccatcg tgctgttctg cgcggtgggt gtgtactcca ccaacaacaa caccttcgac
    31741 gtgtggctgg tcggcgcctt tggctttgtg ggctacatgt tctacaagct gggggtggag
    31801 cctgcgccgc tgctgctggg cttcatcctc gggccgatga tggaagagaa cctgcgccgt
    31861 gcgctgctgc tctcgcgcgg cgactggagc gtgttcgtga cgcgccccat ctccgcaggc
    31921 ctgctggccg ccgcagcgct actgctggtc atcgtgctgc ttccggcggt gagcaagcgg
    31981 cgtgacgagg ccttcgtcga ggaggattga cggcgtccgt accacccctc caggcatgca
    32041 cggcacccgc gggtgccgtg ctgcgttctg gcgcctgcat tgcgcacaca atagcccccc
    32101 atgcccctgg actacctccc cgcacaacac ccgcaacgcg cgcagctgca caacgaaatc
    32161 catgcccgcc cgcccgaggc actggcggcg ccggtggcga ttgcgcatat cgtcatgtgg
    32221 gccgacgccg cgcagcgcga ggccagccgc gcgcacctgg cggccctgct gcgcgaccag
    32281 caccaggcca tgcccgatgc acacagcacg catctgcgtg ccaacctggg cgggtttcgc
    32341 ctgcgctggg agctgcacac cgagttcgtc acctggacct tcagcgtgcc gctgccggcc
    32401 gatgcctgga accaacgcgc cccagccgcc gccaccgagg ccgtgccgcg cgagtggctg
    32461 tcgcagctgc ccggcctgtg cctgtgccag ctgcagctgt gggtgctgcc gacgcgcgaa
    32521 tatgacgagc tgccgcagct cggcgagctg ctgcgcgagg actcgctcgt tgcctccagc
    32581 gtggcccagg gccatggcga ggtgttcacc gacttttgcg tgcatgccga cggcagctcg
    32641 cgcatggtgc tgctggccgg cagcatggcg ccgcggcgcc tggggcggct ggtgcagcgc
    32701 ctgctggaga tagaaaccta ccgcatggcg gccctgctgg gcctgcccgt ggcacgcgag
    32761 gccgcggccg tgctgggccg cgccgagcag gagctggcgg cgctggccga cgccatacgc
    32821 tcggccgacc gcgacatgga gcccgagctg ctggaccgcc tgacgcgcct ggcgggccag
    32881 gtggagagcc agtacgccgc cacccaggcg cgcttttcag cgagcagtgc ctacttcgag
    32941 ctggtggatc tgcgcatacg cgacatcgcc gagtcgcgcc tgggcggcat gcagaccatt
    33001 ggcgatttca tggaccggcg cctgagcccg gcgcgcagca cctgcgaatg ggtggcacgc
    33061 cggcaaaatg cgctgagcca gcgcgtctcg cgcatcagca acctgctgcg cacgcgcgtc
    33121 gagatcgagc agcagcaaag cagccaggcg ctgctggcca ccatgaacca gcgccaggat
    33181 ctgcagctga agctgcagtc cacggtggag gggctgtcgg tggcggccat cacctactat
    33241 atcgtcggcc tggtgagcta cctggccaag ggtgcgcaaa aactgggctg gccctggtcg
    33301 cccgagagca cggcggccgt cgccatcccg ctggtggcgg gtgccgtctg gtggtcgctg
    33361 cgtcggctgc accagcgcat gttcaaacac tgaccaagga gcgaagcatg gatttcggta
    33421 tcgaaggcaa atgggccctg gtctgcggcg ccagcaaggg cctgggcctg ggttgcgcgc
    33481 aggccctggt gggcgagggc gtgaacctgg tgatcaacgc gcgtgcgccc gatgcattgc
    33541 accaggttgc ttcacaatta atagctattg gcgccgatag ggtaagcgct agaggccaaa
    33601 atggcttgaa accacaggtg ctggccgtgg ccgccgacat caccacaccc gagggccgcg
    33661 cggcggtgtt cggcgccgcc ggcggcccgg ggcgggactt cgacatcgtc gtcaccaacg
    33721 ccggcggccc gcccaccggt gactttcgcg actgggagcg cgaggcctgg atacgcgccc
    33781 tggacgccaa catgctcacc cccatagagc tgatgcgcgc cacggtggat ggcatggcgg
    33841 cgcgcggctt tggccgcatc gtcaacatca cctccagcgc ggtgaaggcg cccatcgaca
    33901 tcctgggcct gtccaacggc gcgcgcagcg gtctcaccgg ctttgtggcc ggcgcggcgc
    33961 gcggcggcat tgccgcgcat ggcgtgacca tcaacaacct gctgccgggc aagttcgaca
    34021 ccgaccgcct ggccgccacc ctgcgggccg cggccgacaa aagcggcaag agtctggacg
    34081 aggtgcgcca ggcccagcgg gccgcgatac ccgcgcggcg ctttggctcg ccgcaggagt
    34141 ttggcgccat ctgcgccttt ctgtgcagcg cgcaggcggg ctatatcacc ggccagaaca
    34201 tcctggccga cggcggcgcc tatcccggca tgctgtgagt ccggatcggt catagcggac
    34261 ctgtttccaa ttgtttgcgt gacaaatttg atccccatga atcaggcagt tcttgactga
    34321 tttgcgtcac gattgataca tttctcaaca atcctccaaa gggggacatc aggagtggac
    34381 gcaatgggct ggcatgtggg aacaaggaca accggagcta tcgccctggc cgcgggcctg
    34441 tgcgcggctg cggcgcatgc cgtggcgcca gcacatcagt ttcgtgtggt gggtgggctg
    34501 gccggagtga accaatacac ccaatgggag gagcccttct ggacgcgcga actgccgcgc
    34561 atcagcggcg gccgtttcaa ggccgagatc gtgccctttg accgtgcggg cgtgcccggc
    34621 gaggacatgc tgcgcctgct gcaactgggc gtggtgccga tggccaccgt cctcatgagc
    34681 ggtctgaccg cccaatatcc cgagtacacc gcccccgacc tggcgggcct caaccccgac
    34741 atggccagcg cccgtgcggc ggtggcggcc tttcggccct tcctcgagaa aaacctgcgc
    34801 gaacagcatg gtgtgcgctt gctggccgtt tacgcctatc cggcccaggt ggtgttttgc
    34861 aagaaggcgt ttagcgcact gaccgagctt gcggggcggc gtgtgcgcgt gtcgtcggcg
    34921 gggcaggccg acttcatgga ggccctgggg gccaggccgg tgcagttgcc gtttgcgcgg
    34981 atcgtggcgc aaatcgaatc ggacagtatt gactgcgcca tcaccggcac gctgtcgggc
    35041 catggcctgg ggctgcacca ggtcaccagc catttgcacg ccatgccgct caactggggc
    35101 ctggcgctgt ttggcgccaa ccagggcgcc tgggaggcct tgcccgccga gctgcgccag
    35161 ctgctggcca gcgagctgcc caagctggag gaggcgatct ggacacagtc ggagagcgac
    35221 acccggcgag gcatggcctg caataccggt caggctgcct gcgcccaggg gcgcaagggc
    35281 gccatggcgc tggtgccggt gacgccacag gatgagcgcc gcaggcagga gattttcacc
    35341 aacaccgtgc tgccgcgctg gctgcagcgc tgcggccagc gttgtgccga tgtctggcgc
    35401 cagactcttg ggccgaccct gagggcaaac ccgccatgac gggcgaggtg atggtttcat
    35461 gcgcctgaat cctgcccgcg taccggtgct ttttgggctg ctggcagcgc tgcttgtcgt
    35521 cgtgctggcg ggcagcgccc tgggtctggt ctggtccatg cgaaccaccg ccatgcgcaa
    35581 cgcggagggc ctggtcgaga acatggccat gggggtggag gcgacgctga accgccacct
    35641 gctgtcgctg gacttgctgc tctcaggggt gcaggatcag ctgcaggtga cccatgccaa
    35701 tgatcgatcg gacgatggcc gtctgctgac catgatctcg cgggacaacc tgctcgtcgg
    35761 tcggctgctt ctgctggatg ccaagggggg cgtgcatgca gtgtccggta gttcaggggc
    35821 gctgcaggaa ttttcactcc cccctgcgtt ttgggccgag atcacgcagt ccaaggcggc
    35881 gcgcatgcac atcagtggac cggtgcaaag ttttgcctcc gcccagcagg tgctcttttt
    35941 tgcccggcgc ctgagtctgg acggcatgcc gtattgggcg gtggccgagg tgccgctcgc
    36001 caagttcacc gaggtggcgc agggtagtga acggtcgggt tttgaagtgt tgctggagaa
    36061 aaccaatggg cagcgtctct tcgccgtacc tgacctgccg gtatcggcag gtcaggactt
    36121 gatggcgcat tccccattgc cggagcgtgc ctggaatgtc ccggcacgca tcagcggcgc
    36181 gccggcgctg gtgctggggc gttcgctgct gtacccggag ctgcgctttt cggtcagtct
    36241 gcccatggcg ctggccctgg cacgctggca ggccgagcgc aatgccgtgc tgctggtggt
    36301 ggcgctgttt gccacggtct tgttgctggc ggctgtggtg gcggggcggg tgttccggca
    36361 gttgagcgct gcgcggcaga acacggccgc atccaaggcc atgctggacg aggcgctgga
    36421 gtccatggtg ggcgggtttg ttttgcttga cagccagcgg cgcgtggtgc actggaaccg
    36481 gcggttcgtg gtcatatacc cctggctggc cccctatgtc gccccgcaac tgccgtttcg
    36541 cgtcctgctg gagcagtcgg ccagaaatct gttgcctgcc gcctcgcagg ccgaacgcga
    36601 acagtggatt caagaacggc aggcgcagca gcgtgaaggc atgggctccg cggagcagct
    36661 gttgcccgat gggcgctgca tccagctgca ggagcggcac acccccgacg gtggtttggt
    36721 gatcacctac cacgacgtga ccgacctgcg ccgcgccaag gaggagatag agaacctggc
    36781 cttctacgat gccctcaccg gcctggccaa ccgccgcctg ctgctcgacc gcctgggccg
    36841 ggccatcgtg caggcccagc gcacggggaa ggtcggtgca ctgctgtttc tggatctgga
    36901 tcacttcaag gccctcaacg acagcaaggg ccatgaaatg ggcgatgaac tgctccagca
    36961 ggtggcccgg cgcctgctgg cccatgtgcg cgagatggat accgtggcgc gcctgggggg
    37021 cgatgaattc gtcgtcatgc tgcgcgacct gtccgccagc agggagggcg cggcggccgt
    37081 agcacagcgc attgccgaga agctgctgca ggccctggaa gaaccgtttc aactgggcgg
    37141 ccacctgtac cagggggccg ccagcattgg cgccacgctg ttctgtgcgg atgtggacag
    37201 cgccacagac ctgctgcgcc gcgccgacat tgccatgtac caggccaagg cccggcgcgg
    37261 caacgccctg tgtttcttcg acccccagat gcaggcggcc atcaacgagc gcgcgcagct
    37321 cgagtctgac ctgcgccagg cgctggccag cgaccagctg gtgctgcact accagccaca
    37381 gtgcacggca agcggcgatg tgctcggggc cgaatgcctg gtgcgctggc agcaccccga
    37441 gcgcggcctg gtgccgccgg ggcagttcat cccggtggcg gagggcagcg atctcatcct
    37501 gggcataggc caatgggtgc tgcgggcggc atgccggcag ctggccgcct gggcccatca
    37561 ggccgactgt gcgcggctgc ggctgtcggt caacgtgagc gcgcgccagt tccgccagga
    37621 cgatttcgtg tgccaggtcg accgcgtgct gcagaccacg ggggcgtcgg cccatttgct
    37681 gacgctggag ctgaccgaat ccatggtgct cgacaacgtg caggacgcca tcgagaagat
    37741 ggatcagctg cgcgccaagg gcgtgcagtt tgccctggat gactttggca ccggctactc
    37801 gtcgctgtcc tacctgacgc gcctgccgct gcacacgctg aagatcgacc agtccttcgt
    37861 gcgccacctg ggcgagcggc ccgcggacaa cgccatcgtg cagaccatca tcggcatggg
    37921 ccgcaatctg gatctggcgg tcatcgccga gggggtggaa acccaggcgc aaaaagacct
    37981 gctggccggg tacggctgcg acctgtacca gggctacctg ctcgggcggc cggcaccgat
    38041 cgaggtgctg caggcgttgc tggcacggga ggtggcctca gcggcgtgag gacaccacga
    38101 tgccgccgac gataagcaca aaggcggcgc cgtggtacag gtgcggcgcc tcgcccagaa
    38161 aggccgccga cagcagggcc gcgaacaggg gcgtcaggtt gatgaaaaaa cccgccgcca
    38221 ccggccccgc gcgctgcacg cccgcgcccc agaaacggta ggccaggatg gccgggccga
    38281 tgacgatgta agccaggccc gcggccaacg gccagcccca ggctatgtgt ccctggccca
    38341 gcgaccattc cgcgcccgca aaggcgcccg accagcccag gccgaagaac atctgcgctg
    38401 tcagaaaggc cgcccagtcg ccgcgcaggc cctggggctc gctggtgcgc accagcagcc
    38461 agctgtagaa cgcccaggag atggtggcca gcaccatgta cagatcgccc ggcaccaggc
    38521 gcagcgccaa cagctcgcca acactgccac ggctcagcac cagcaccacg ccggtcactg
    38581 acagcagagc gcccaccgcc tgccggcgcg tgaccggcac gccgaagaac agcgcgccca
    38641 ccaccagcat ccacaccggc atgccagagc ccaccagcgt cacgttcagc gccgttgatg
    38701 tctgcagcgc caggtactgc agcgcgttgt agctgccaat gccgaacatg cccagcgccg
    38761 catagcggcg ccagtgcggc cacaggccgc ttgccgggcg cagcacctgc caggccagcg
    38821 gcaacagcag cgcaaaggcc agcagccagc gcaggaaatt cagggtgctc ggggcaatca
    38881 gggtgtgcag cgcgcggccc accacggcat tgccggccca caacaggggc gcggcggcaa
    38941 gcatggcggc cgtgcctggc gagagtcttt gggtcatagg ggctgcgact gtaagccagg
    39001 ttctgtatcc cgcgtcgggc aggggccagg caaacatggc acgatcctgg ccatcgcaat
    39061 gcacgcagga gaactggatc catgaccctc gccgtacaaa tccgccagca tggcggccct
    39121 gaagaactgc agatcgttga cgtgcaggtg ggcgagcccg gcccgggcca ggtgcgcata
    39181 cgccaccacg ccgtgggcct gaacttcatc gacgtctatc accgcaccgg cctgtatccc
    39241 ctgaacatgc ccgccggcat aggcatggag gccgcgggcg tgatcgaggc cgtgggggag
    39301 ggcgtcacgc acctgcaggt gggcgaccgc gcggcctatg ccagcaaccc gccgggcagc
    39361 tactctgagc gccgtgtcat gcccgccatg tgtgtgtgca agctgcccga tgccatcagc
    39421 ttcgagaccg gcgcggcgat gatgctcaag ggcctgacgg cgcagtattt gctgaaaaag
    39481 acacgccccg tcgaggggct tgagccaggc gacttcgtgc tgttccacgc cgccgccggc
    39541 ggtgtgggcc tgatcgcctg ccagtgggcc agggcattgg gcctgcggct catcgccacg
    39601 gcgggctcgg acgccaaatg ccagctggcg ctggacaacg gcgcggccca tgccatcaac
    39661 tatcggcagg aagactttgc cgcccgcgtg aaggagatca ccgcgggcca gggcgtgaag
    39721 gtggtctacg actcggtggg caaggacacc tgggacaagt cgctcgattg cctgcgcccc
    39781 ttcggtttga tggccagctt cggcaacgcc tcggggccgg tggcgccgtt tgcgcccggc
    39841 atgctggggg ccaagggctc gctctacgtc acgcgccaga cgctgttcac gcatatccgc
    39901 acgcggggcg ccacgcaggc catggccgat gagctgtttg ccgtggtcgc cagcgggcag
    39961 gtgaagatcc acattgccca gcgctacgcc ctgaccgacg tgcagcaggc gcaccgggat
    40021 ctggaggcgc gcaagaccac gggctccacc ctgctcacgc tgccctgatg gccgccacca
    40081 cggcgcccgc ctgggtggcc gccgcacgcg cgcgtgccac ttgcgcgccg gcagcgccac
    40141 gcatggcgct gctgctgggc ggtgagcgcg tgggctcggt ggatggacag atttttaata
    40201 aaattggcct ccagcgcttg ctggataagc gctaccagct atcaatagta gagtatgaag
    40261 gcgcccctgc ctgggccttg gccgccgacg atgccacggc cgcgctgaat gacctggcgc
    40321 tgaccctgcg cgccgccggc cgctgtggcc cctggcgcga cgagcaactg gccgtgtgcg
    40381 ctgccgatgg ccggcgcctg gccacggtgg agcgtggcgc cgcgcgcgtg ctgggcctgg
    40441 ccacccaggc cgtgcacctg gtgggctgca cggccgacgg cgccgccatg tgggtgcagc
    40501 agcgcgcgca cagcaaggcc agcaacccgg gcatgtggga cacgctgatg ggcggcatgg
    40561 tgtcggccgc cgacagcctg cagaccgccg tggcgcgcga gacctgggag gaggccggcc
    40621 tgcgcatggc ccagctgtct gggctgaggc cgggtggcca ggtgcggttt gccctgccca
    40681 gcgacgaggg cgggggcgcc ggctacatgg tggaatgcat ccactggtac caggccaccg
    40741 tgcgggctgg ggccacgccc gacaaccagg acggcgaggt cgagcgcttt gattgcccgg
    40801 cgcacggcga ggtgcaggcg cgcctggcac agggcgcctt cacgcccgag gcagctttgg
    40861 tgctggccgc cttctacggc tggtagcgca gtctcacagc cggtcgttct gcgtgctggg
    40921 cacctcggcc tccgggcgca gcggggccac ggcgttgctg gcctccagca ccgggtaggc
    40981 caccgagcac agcagcgaat tgatgcgctt caggtcgctg atgatgtcga tgtgcagcga
    41041 gctggtctct atgctctgga tggtgttttc cgccaggcgg ttgaggtggg tggcggcgta
    41101 ggccagctcc agctcgcgga agcggttctt ctcctccagc agcttttgcg catcgcgcac
    41161 attgctggtc agaaacacgc tcatggccag gcgcaggttg gccaccaggt ggtcgtgcat
    41221 ctcgttgatc tcctgcagcc cggcatgcga gaactgccgc cccttcttga tcttcttgtc
    41281 ctccacgtcc tgcagcacgc gctccaccag gtcgcccacc tgctccatgt tgatggtgaa
    41341 gccgatgatt tccgtccagc gccggccctc ggcctcatcc agctcggagc gtgagatctt
    41401 ggtcatgtag tacttgatgg ccgagtacag gctgtccacc tggtcgtcca ggccgcgcag
    41461 ctcggcgccc aacttgtcgt cgtcggtctg ccacacggtc ttcaggccat tgagcatggt
    41521 ttccaccaga tcggccaggt gcagggcctc gcgtgccgcg caggaaatgg ccagcgaggg
    41581 cgtggacagg gccgaggcgt ccaggtggtg cgggcgcgac agcgaggccg gttgctcctc
    41641 ctggcgcagg atcttgccca cggcggtggc caccaggccg gtgatgggca ggcacagcag
    41701 cgtgcccatg atgttgaagg ccaggtggaa cagcaccacc tggctgacct tgtccgggat
    41761 cagggtctgg atatgcgtgt gccacaggcc tatgaccgga atcatcaccg tggccgccac
    41821 catgcggaac agcaggttgc ccagcggcac ctggcgcgtc tgaatgctcg agcgcatggt
    41881 ggtcagcacc gccagcaggc cgctgcccag gttggcaccc accaccaggc ccagcgccac
    41941 ttcggtgggg atgccgcccg agcccgccag cgtggccacc agcagcaccg tggccaggct
    42001 ggagtaggag atgatggcca ggatggcgcc tgtcatgacc tccaacgtga tgtcgctggt
    42061 cagcgccatg agcagggtgc gcaccacctc ggactgggtc agcaccaggg tggactcggt
    42121 gatcaggcgc agcgccagca gcatcaggcc caggccgatg agcacgcgcc ccacgcgccc
    42181 ggccttgccg tcctggcgcg ccacatacag caccacgccg acaaaaatgc acagcggcga
    42241 gagccaggac aggtccagcg agaacaccac cgccatcagc gccgagccca catcggcgcc
    42301 gagcatcacc gtcagcgccg cggtcaggct gatcatgccc tggccgacga aggccgagac
    42361 gatcagcgcc gtggccgtgc tggactgcac caccgccgtc acccccaccc cggagaacac
    42421 ggccagcagt tgcgtgcgca cgccgcgcgc gatcaattgg cgcaaattgg cgccaaacac
    42481 gcgcaggatg ccggtgcgca ccatttggat gccccacacc agcagggcaa tggcggcgag
    42541 caggttcagc aggtgcttca tatgtggggt tggcgggatg cgagcgctat cagtctatga
    42601 gcatgccatt ttcgtggaaa ggataggggc cttcaaaaat tcctgatgcg gccaggtgcg
    42661 acaccaggcg ctggccgtcc caggc
//
LOCUS       DAOMTQ010000007        34418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_83_length_34418_cov_24.064779, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000007 DAOMTQ010000000
VERSION     DAOMTQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 34418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34418
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_34418_cov_24.064779"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PK518_01640"
     CDS             complement(<1..478)
                     /locus_tag="PK518_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRM47642.1"
                     /translation="MIPIYPDNSTSLAKLDAMLQDDPANHLYRLDRAAFTDEQLFELE
                     MKYIFEGNWVYLAHESQIPNINDYLTVYIGRQPVVITRSKDGKLNALANTCTHRGAIL
                     CRFKKGNKSSFTCPFHGWTFSNNGKLLKVKDNTPDAGYPPSFNCEGSHDMVKLGAFE"
     gene            1126..2943
                     /locus_tag="PK518_01645"
     CDS             1126..2943
                     /locus_tag="PK518_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HRM47643.1"
                     /translation="MKKSSTGSPADLSLPLIHADHTDIDKVCFPNLVDLMARLHFSTS
                     DGRIWLDEQRMLLVHAKALGSMRREMIESLGTDVARGLLTRMGYQAGTYDAQMARKVR
                     SKTSLKDMFVVGPQMHCLEGIGLSEPVRLEFDVGKGEHYGEFLWTHQVEDEEHVRHFP
                     IGTEPSCWMQVGYASGYSTEFMGRQILYREVECQSMGQTACRIVGMPVEAWGDEGLQD
                     LQHLMPAALRSDAPEAAVSATRNPVLLGADATDSAQPVGISPGYNAVLHMVQRVAPTN
                     ATVLFLGETGVGKEVFARMLHRMSSRSHKPFVAVNCAAIPETLIESELFGTERGAFTG
                     ATQSRAGRFERADGGTLFLDEIGTLSAGAQGNLLRALQEGEIERLGDTQTRRVDVRVI
                     AATNVDLREEVAAGRFREDLFFRLNVFPIRVTPVRERREDIPLLMTHFLAKFNRLHGR
                     RLTGFTQRAVDAVMSYAWPGNIRELENVIERGVILAAQNTAIDAPQLFTSGEQFNRKQ
                     FAVSPDGLLVHADQTRLLLEEPPVDPEIDRVSKHINNLLNGIEDDSKVSLDDIETLLL
                     KQAVTRSQGNVARAARLLGITRPAMVYRLKSRGILHPGQ"
     gene            2971..4206
                     /locus_tag="PK518_01650"
     CDS             2971..4206
                     /locus_tag="PK518_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRM47644.1"
                     /translation="MFSHLERPSGPIPHWRQVLADFGPREAANGFIGWVFSATAPVAI
                     ILAVGTRGGLSEAELASWVFGVFFINGLISIFFSWLYRQPLAFAWTIPGTVLVGPALG
                     HLSFAEVIGAFYVTGALMLALGATGWVKRAMRAVPMPIVMGMVAGVFLRFGLDLIRSL
                     HEDALIVAPMILVWLLLTVFPALGRRLPPLIGALLIGALVVILAGRFDAASLGALEFA
                     RPVIHAPAWSWGAIFELVVPLAITVLVVQNGQGMAVLKAAGHEPPVDAITVACGVGAM
                     ASAVVGSVSTCLTGPTNAIITSSGEQSRHYTAAIFCGVLGVIFGLLSPAFTRLMLHAP
                     KAFIMALAGLAMLRILQAAFVTSFKDRFTLGALVSFLVTVADLGLLNIGAAFWGLVAG
                     FVISWLLERPDFVAVANVS"
     gene            complement(4347..4856)
                     /locus_tag="PK518_01655"
     CDS             complement(4347..4856)
                     /locus_tag="PK518_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRM47645.1"
                     /translation="MQHLLNLLALPQFGLSTVFVVAFVSATLLPLGSEPAVFGLVKLN
                     PELFWPAILVATLGNTLGGALSWWMGRGAHRAWDAARRRRQAGQPASAELPAPRELSR
                     TERRARVWLRRWGPKTCLLSWLPVVGDPLCAVAGWLRLPFWPCLAYMAVGKLARYLVM
                     TTGLLYLVH"
     gene            4986..6041
                     /gene="dusB"
                     /locus_tag="PK518_01660"
     CDS             4986..6041
                     /gene="dusB"
                     /locus_tag="PK518_01660"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRM47646.1"
                     /translation="MHIGHIPLANRLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTS
                     RRDLWASLKTSRRANHEGEPGPIAVQIAGTDAAMMAEAAVYNVERGAQIIDINMGCPA
                     KKVCNKWAGSALMQNEALAVEIAAAVVAACAPHRVPVTLKMRTGWCQQQKNAVQLARQ
                     FEAVGVQMLTVHGRTREQGYKGQAEYDTIAAVKAAVAVPVVANGDITSPEKARAVLAQ
                     TGADAIMIGRAAQGRPWIFREVAHFLATGEQLAPPLVAEVRRLLLEHLQDHYGLYGQA
                     SGVRSARKHIAWYVRDLPGGEAFRQQINTMEDCAAQWQAVADFFDALGAQMDRLPLAS
                     DDADPTTRESMHEQPQY"
     gene            6022..6255
                     /locus_tag="PK518_01665"
     CDS             6022..6255
                     /locus_tag="PK518_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HRM47647.1"
                     /translation="MSNHNIEQCVRESLQSYFRDLDGETPDGVYDMVVRLVEKPLLEV
                     VMNQAENNQSRAAEWLGLNRNTLRKKLLDHKLL"
     gene            6338..7939
                     /gene="purH"
                     /locus_tag="PK518_01670"
     CDS             6338..7939
                     /gene="purH"
                     /locus_tag="PK518_01670"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRM47648.1"
                     /translation="MNALLSVSDKTGIVEFARALHALGIRLLSTGGTAQLLAREGLPV
                     TEVAEVTQFPEMLDGRVKTLHPKVHGGLLARRSLPAHMAALQQHGIDTIDLLVVNLYP
                     FEATVAKAGCTLADAIENIDIGGPAMVRSAAKNWQDVGVVTAADQYAGVLAELQAHGK
                     LSDKLRFALSVAAFNRIAQYDGAISDYLSSVQFEEEKLNDAYVPERSAFPGQSNGHFI
                     KVQDLRYGENSHQSAAWYRDLHPAPGSIATGVQLQGKELSYNNIADADAAWECVKSFK
                     LPACVIVKHANPCGVAVGTSALEAYSKAFQTDPTSAFGGIIALNRAVDGAAAQKIAQQ
                     FVEVLMAPDFTPEALEVFKAKANVRLMKIAIPAPGGATAWEQGRNAMDAKRIGSGLLL
                     QTADNHELQLPDVKVVTLKQPTQEEMQDLMFAWKVAKYVKSNAIVFCKGGMTMGVGAG
                     QMSRLDSARIASIKAQAAGLSLQNTVVASDAFFPFRDGLDVVVDAGATCVAQPGGSMR
                     DQEVIAAANERGVAMVFTGVRHFRH"
     gene            complement(8179..9483)
                     /gene="hemL"
                     /locus_tag="PK518_01675"
     CDS             complement(8179..9483)
                     /gene="hemL"
                     /locus_tag="PK518_01675"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRM47649.1"
                     /translation="MTHSTDHNQTLFERAKALIPGGVNSPVRAFRAVGGTPRFIARAQ
                     GPYMWDADGKKYIDYIGSWGPMILGHGHPAVLEAVQKAALEGFSFGAPTEREVELAEE
                     IIRHVPSMEMIRLVSSGTEAGMSAIRLARGATGRSKIIKFNGCYHGHADSLLVKAGSG
                     LATFGHATSAGVPPEVVQHTLVLEYNDVAQLEEAFALHGKEVACVIMEPIAGNMNFVR
                     ASTAFMRRARELCTQNGALLVIDEVMTGFRVALGSAQGLYAQQLPGFKPDLTVLGKVI
                     GGGMPLAAFGGPRAIMEQLAPLGPVYQAGTLSGNPVATACGLATLREIAKPGFYEALA
                     TRTRSLVDGLAGAAKAAGVPFCADSQGGMFGFFLLPELPQNYPEVLKTDGAKFNQLFH
                     GLLDGGVYIAPALYEAGFVSAAHSAQDIADTVALAREVFAKL"
     gene            complement(9467..10399)
                     /locus_tag="PK518_01680"
     CDS             complement(9467..10399)
                     /locus_tag="PK518_01680"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRM47650.1"
                     /translation="MSTMTSSSQDNPAPADDDAVLPAICVLAFNANDPCGAGGLAADI
                     AAVASVGGHPAPVVTGAYIRDSAEIHDHCAFDDDAVADQARAVLEDMAVQAIKVGFVG
                     SPENLSAIAEIATDYPEIPVVAYMPDLSWWHDDLIDQYQDAFLELLLPQTSVLVGNHS
                     TLWRWLLPDWSSERAPTARDLAMAVADSGVPYLLVTGIPAAEQHIENTLTSAQTVLGS
                     GRFERFEATFIGAGDTLSATLAALVASGNDLGEATSEALTYLDGCLEHGFRPGMGHVL
                     PDRMFWAEPDEDDNSEEPAPTAADFDFPPHDTQH"
     gene            10515..10667
                     /locus_tag="PK518_01685"
     CDS             10515..10667
                     /locus_tag="PK518_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRM47651.1"
                     /translation="MCVVCGWIYDEAEGCPEDGIAPGTRWADIPASWTCPECGAGKQD
                     FEMVEI"
     gene            10735..11133
                     /locus_tag="PK518_01690"
     CDS             10735..11133
                     /locus_tag="PK518_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM47652.1"
                     /translation="MTTSDTTFRVLVVDDSNTIRRSAEIFLKQGGHEVLLADDGFDAL
                     SKVNDYQPQLIFCDILMPKLDGYQTCAIIKRNARFSDTPVVMLSSKDGVFDKARGRMV
                     GCQEYLTKPFTKDQLLLAVQQFANAQQGVM"
     gene            11133..11498
                     /locus_tag="PK518_01695"
     CDS             11133..11498
                     /locus_tag="PK518_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM47653.1"
                     /translation="MAIRKVLVVDDSKTELLYLTDLLQKKGMQVRTAENGEEALRRLA
                     EDKPDLILMDVVMPGQNGFQLTRSITRDPLYADVPVIMCTSKNQETDRVWGMRQGARG
                     YITKPVDPAELQAKIDALN"
     gene            11503..12030
                     /locus_tag="PK518_01700"
     CDS             11503..12030
                     /locus_tag="PK518_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRM47654.1"
                     /translation="MANREALKELQTRLAARLQAAKAEGVAVASWLAVESGAQHFLLP
                     LAQSGEIFSWTGVQPVPYTQPWFLGVANLRGTLTGVVDLAGLLGGSPARPEQALFESS
                     LLALNAALEVNAALLVDRLVGLRGAQAFVASEPPAAEAAAYWGNTYVDAQGTRWQELN
                     LQALAQHPTFLSISA"
     gene            12049..14292
                     /locus_tag="PK518_01705"
     CDS             12049..14292
                     /locus_tag="PK518_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM47655.1"
                     /translation="MSVVNPLEKLFLRKPVQGDADLGQPDIIQDSISAGVLSQAYQAD
                     GMPSVLGDYGDTVRKPEPGVAEQEQISLPLLGSASTGTHQRRLLILLGVGLVLLALIA
                     GWALQQSSRSAKQLAAMGQSLMQSQRLAKSVSQALAGQPQAIADMKDSADVLARNLRA
                     LNAGDAELGVQALGESFKPELEAMNPLVERAERNAGLLLGQKAILTQVGEALRAINQQ
                     SPALLESAEGIAALKLQNAAPAADISAAGQLVMLTQRIGKSANEFQAAEGVNPESVFL
                     LGKDLNSFKEIAQSLLAGTRDAQTREQLQALITLFDQTRTQAGAILGNLQGLAAARQA
                     QAAIVADSEPLRRQLETLQDKLSAQSGVGPGQIAALVVAGLFVLLCGVGVSRVQLLDS
                     RGRQVAAEALQIDAKRQEQEAKRVNDANQAAILRLMNELQSVAEGDLTQEATVTEDIT
                     GAIADSVNYTVEELRLLVGSVQNTAARVAQTTAQVDSTSTELLAASTEQLREIRETGR
                     SVLDMASRINQVSSQAQESAAVARQSLQAADQGLRAVQNTIGGMNAIRDQIQDTSKRI
                     KRLGESSQEIGEITELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAER
                     SADATRQISALVKAIQTDTQDAVAAMERSTQGVVEGARLSDSAGTALSEIDSVSRRLA
                     ELIEQISSSASKEAELANGVADNIQHIFAVTEQTGEGTRATAQQVRELSRMADELRQS
                     VARFKIA"
     gene            14338..20274
                     /locus_tag="PK518_01710"
     CDS             14338..20274
                     /locus_tag="PK518_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRM47656.1"
                     /translation="MSSLDAATATGADGTQNEQDLGPLAWVLDELRKSLEGVAKSMRR
                     FVRDAEMARQSDLAALDAAALRIARQQLHQAGGALTMVGMESPALLLRAMEAAVQRFV
                     HRPELCSDDAARTIERASFALIEYLECVLAGQRISPVALFPQYRDVQALAGVDRVHPA
                     DLWPMNAPVQAPALPVGVAPLPYGAEARSRLDSAVLRIVKNADPGAAADMRAISQGFA
                     AAQAEPQVRMFWTVCAGFFEALAQQALKPDLYAKRVASRVLMQYATLAKGEHGITERL
                     LQDLLFFCAQAAPEPTAAAATPALQAVRQSFGLQRHPPVDYERPRFGLFDPALLAQAR
                     KRIAAATETWSALAGGDRNKLKPAVDQFSLVCDSLRKLCPESERLAQALTHVLDVTLR
                     SGEPPPAALAMEVATAVLYLQASFEELDASRDAMGQRAERLAERLDSVVAGGQSQPLE
                     SWMEELYRRVSDQQTMGSVVGELRATMAEAEKSMDQFFRQPDDLAPLAGVPGGLQQMR
                     GVLSLLGLDQASLAVVRMRDMVERLLVGEVAPEERQQVFEKLGNSLGALGFLIDMLSY
                     QRAMARKLFVYDQELGELRIVMGRAPVGQPEPSAAAPAQAQSPAPESEPEPAPQAVVP
                     QPAEPAPLDLAVHAAPVLPQPEVAPTPLVPVAAVTLPDAGADDDADDDAELLDIFLEE
                     AREVVATGLAAVQALHAEPGDLSEQTTLRRAFHTLKGSARMVGLAEFGEAAWAMEQTL
                     NAWLAEEKPMSAPLLQLSDEALQAFGRWADDIEAGVAGHWQALPFGAAATAMREQGTL
                     LPLTAASEFLMEVEPSPEPALPPVVADEAESAPSANAEPGLEDHGDGPQQDFADTCIE
                     LPDFAASVPPAVEVTETVLLDELDFAVFEQALQAQADDVTEPLPESTGLAVADTAAEE
                     VLHQPSPLDDGFALDIPVDAVEGVGQPGEAEPPEAVAAGDEADESEALEVLPSTDEAI
                     KVIEHLRIGIPLYNVYLNEADEWSRRLATELQEWSLELHRPVPEAAVALAHSLAGSSA
                     TVGFAALSDLARQLEHALQHVQLLPHGLAEHAQACNAAADDIRRLLHQFAAGFLKEPN
                     PSVRERLAAVLRIEVSSGAAPLEAMAQSPDTLELEFGDLAMQDEVADLPTEPSGQTSE
                     PADDQPDAAGQDLEAGFEHDRAIDEAIARAVAAVDDIDDDIDALDVIDPDLFPIFEEE
                     AAELLPALGSALRQWAARPENLSARNELLRALHTLKGSARLAGAMRLGEMAHRMETAV
                     EQIGTNGVTTAAVEPLLASLDVLQADLDVLRTIGAQPLSEPMVVSAPEAATPASAAAV
                     PQLPAAAAPVAAPLSWRVPASASATVPAQRAATQSVRVRAQLLDRLVNQAGEVMISRS
                     RLDARLGQMRASLADLTGNLERLRQQLRDIEVQAETQMQSRLALSKDVAAGFDPLEFD
                     RFTRVQELTRMMAESVNDVATVQRNLQRSMEGAEDDLIAQGRQARELQRDLLRTRMVE
                     FESIAERLYAVVRQASKETGKQIKLDISGGSIEMDRGVLDRMAPAFEHLLRNCAGHGI
                     EAPETRVAAGKPAVGTITIALRHEGNDVSVEFSDDGAGLDLERIRARAVAQGLIAPDE
                     PITATQAANLIFRPGFTTASEVTGLSGRGIGMDVVRAELNALGGRIETQTEAGKGSSF
                     RMLLPLTTAVTQVVMLRAGGLTLGVPANLVEVVRRSPVAQVDAAYASQWFAEGSESLP
                     FYWAGALMQSAPRSTEVAGKTRPVIILRSASQRLALHVDEVLGNQEVVVKNLGPQLSR
                     LPGVAGMSVLASGAVVLIYNPVALATVYGEQARAAMLAPAAQDAGMVADITRAGMSAP
                     ASSVAQLDQVPLVLVVDDSITVRRVTQRLLQREGYRVALATDGLQALARLQQERPAVV
                     LSDIEMPRMDGFDLARNIRADAALADLPIIMITSRIAQKHRDHAAQLGVNHYLGKPYS
                     DEELLSLVGHYAQLTVAQGG"
     gene            20351..20971
                     /locus_tag="PK518_01715"
     CDS             20351..20971
                     /locus_tag="PK518_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRM47657.1"
                     /translation="MSATSAPMNLTHHFLIAMPGMEDASFARSVVYLCEHSERGALGL
                     IINKPTDISLEGLFEKVDLTLGRADLTHQPVFLGGPVQTERGFVLHDPMRGTPQGAST
                     PDADEDDSPYASTMSIPGGLEMTTSKDVLEALSHGAGPRRVLVTLGYSAWGEGQLESE
                     LAENSWLTVDADAAIIFDTPVGERYERALGLLGLQSWMLSRDAGHA"
     gene            20968..21387
                     /gene="ruvX"
                     /locus_tag="PK518_01720"
     CDS             20968..21387
                     /gene="ruvX"
                     /locus_tag="PK518_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRM47658.1"
                     /translation="MSGAPAPAAVPAHFQSFLAFDFGLKRTGVAVGTRMLRTATPQGT
                     IRAEGDARFAPIAQRIAEWQPDALVVGVPFHPDGAPHENTARAQKFARQLRGRFGLQV
                     FEVDERYSTTEAQAAGALDADAASACIILEQFLRSLA"
     gene            21384..21896
                     /gene="pyrR"
                     /locus_tag="PK518_01725"
     CDS             21384..21896
                     /gene="pyrR"
                     /locus_tag="PK518_01725"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRM47659.1"
                     /translation="MNTTTGSALLLDAEALYGELLRGVRALLQPDTRLAGITSGGAWL
                     VERLQRDLGLAGKPGVLSSALHRDDFAQRGMAASAQTQLDFDVNGADILVLDDVLYTG
                     RTVRAVLNELFDYGRPARVRLAVLVDRGGRQLPIAADFAAARVALPASQILALASGAD
                     GSFQFAVKEA"
     gene            21900..22862
                     /locus_tag="PK518_01730"
     CDS             21900..22862
                     /locus_tag="PK518_01730"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRM47660.1"
                     /translation="MLYKRNPQLNKNGELIHLLSIEGLPRDIVTHILDTAANFTSVND
                     REVKKVPLLRGKSVFNLFFENSTRTRTTFDIAAKRLSADVFNLDIARSSTAKGETLLD
                     TIDNLTAMAADIFVVRHSESGAPYLIAQHVGPHVHVVNAGDGRHSHPTQGLLDMYTIR
                     HYKKDFSNLTVAIVGDVLHSRVARSDIHALTTLGAAEVRVVGPRTLVPSDLSHMGVRV
                     FHSLEEGIKGCDVVITLRLQNERMSGALLPSSQEYFKSFGLTPEKLRLAKPDAIVMHP
                     GPINRGVEIASAVVDGPQAVILPQVTFGIAVRMAVMSIVAGNDA"
     gene            22930..24225
                     /locus_tag="PK518_01735"
     CDS             22930..24225
                     /locus_tag="PK518_01735"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRM47661.1"
                     /translation="MKILLQNGRVLDPASGLDKVCHLALAAGRIVGVDRQPADFTPNR
                     VIDASGCLVLPGLVDLAARLREPGHEHEGMLESEMAAAVAGGVTSLVCPPDTDPVLDE
                     PGLVEMLKFRAEKLHQARLFPLGALTRGLAGEVLTEMAELTESGCVGFSQADVALTST
                     QVLQRALQYAATYGYTVWLRPQELYLGKGVAASGALATRMGLSGVPVAAETIALHTIF
                     ELQKTTGARVHLCRLSSSAGVELVRQAKAAGLPVTCDVSINSLMLTDMDIGYFDSRAR
                     LTPPLRQQRDRDALAAALADGTVDALVSDHTPVDEDAKAVPFAEAEPGATGLELLLSV
                     ALKWSRDAGVPLARAVAVVTSEPARVLGSALGTLQASVGRIVEGGVADLCVLDPSADW
                     TVQAKALVSQGKHTPFSSYELPGRVRWTLVGGQVAFDRG"
     gene            24234..24974
                     /locus_tag="PK518_01740"
     CDS             24234..24974
                     /locus_tag="PK518_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM47662.1"
                     /translation="MPRHLRAGWRLLRLLVHALWGLWLVAWRFPRLSSDQQQARVQAW
                     SLGLLTCAGITLQLRGRPQRAGPVLLVANHHSWLDIPVLHAARYCRFISKSDLQDWPI
                     VGTLATAAGTLYIRRESRRDALRMVTSMRDALLAGEVLAVFPEGQTGDGRALLPFHAN
                     LLQAALDAGAPVQPVGLRFLDGIGGATSDAPSFVGDESFLGSVWRTLKAPGITAVVHF
                     GAPERALGRDRRAWSQALHAAVDALRHG"
     gene            complement(25003..26067)
                     /locus_tag="PK518_01745"
     CDS             complement(25003..26067)
                     /locus_tag="PK518_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47663.1"
                     /translation="MFLEVSQLDVRYGSRQTPAVHGVTLGLAAGEIGVLIGPSGCGKT
                     TLLRAVAGLEAVAAGSIRLSGELVGAPGRSVAPEQRRIGMVFQDYALFPHLSVGRNVA
                     FGIHELPRGEQQQRVRECLALVGLEGSEARFPHELSGGQQQRVALARALAPRPRLMLL
                     DEPFSNLDVDLRERLAHEVRGILKTANATALFVTHDQLEAFAIGDRIGVMDGGHLHQW
                     DDAYALYHRPATRFVADFIGHGVFAPATLVRHGGQVVARTALGDLTDIDGCLLPSSYP
                     HGECDVLLRADDVVHDDAAPVRARIVRKAFRGSEFLYTLELEGGLTVVAHVPSHHDHA
                     MGEWIGIRAQVDHVVTFARA"
     gene            26193..27782
                     /locus_tag="PK518_01750"
     CDS             26193..27782
                     /locus_tag="PK518_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM47664.1"
                     /translation="MLALLLALPVLAVLGAWLPLGQGDAQSGAILREMAATVLPDYLW
                     TTLWLSLAVAIGAALVGTASAAAVTLFDFAGRRQLEWLLLLPLAMPAYVTAYAYTDFL
                     QFSGPLQVWLREAFGLQGRLLPEVRSLGGAIWVFIFSLYPYVYLLARTALGERAAHLM
                     EAARLLGAAPARRLRSVALPLARPAVAAGVALVLMETLADFGVASYFGIQTFTTGIYK
                     AWLSMDNRLAAAQLATFLLALVVLLLQLELRAQRRMRFATGGVGRAGSSEAQPTRLHG
                     AYAVLAWLVCALPVLMGFALPVLFMLRPLLADWSLLPWDRFVQWAGNSVRLGAITAVL
                     AVGIALALAFAVRRRRGPLMRAVVQLVSLGYAVPGAVIVVGLLLPVGWLQAAAPTWGL
                     SALVTTTAVGIVWAYLVRFCAVALQSVQSGYARVPASLDDSARMLGVGGMGLLMRVHW
                     PLLKRSTAAATLLVFVDVMKELPATIVLRPFDSDTLAVVAYQLARDERLGEAALPSLA
                     LVAVGLVPVILLSRTLRGSSR"
     gene            27799..28152
                     /gene="arsC"
                     /locus_tag="PK518_01755"
     CDS             27799..28152
                     /gene="arsC"
                     /locus_tag="PK518_01755"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071185.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRM47665.1"
                     /translation="MNDITIYHNARCSNSRGALEILRERGIEPVIVDYIKEPLDAARL
                     TALVARLGVPVRELMRSKEALYQELGLAAPERSDAELIAAIAEHPVLLNRPIVVTPKG
                     ARLCRPPELVLDLIA"
     gene            28220..29740
                     /locus_tag="PK518_01760"
     CDS             28220..29740
                     /locus_tag="PK518_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRM47666.1"
                     /translation="MNTLLTSPRRLVLALVAAGALGATGAGLITAHEARAQLAMPVAA
                     AVAPATPAAAVALPDFSSITARYGPAVVNISVSGMRKTGGDDGDNSPAARQRGPQGMD
                     PNDPFNEFFRRFGIPMPERGGQGQRGMPMRGEGSGFIIDANGIVLTNAHVVKGASDVT
                     VKLTDRREFRAKVLGADPKTDIAVLKIDAKNLPTVKLGNSRDLKVGDWVLAIGSPFGF
                     ENTVTAGVVSAKGRSLPEDSYVPFIQTDAAVNPGNSGGPLFNAAGEVVGINSQIYSRS
                     GGFQGLSFAIPIEVATRVEQQIVATGHVEHARLGVAVQEVNQTFADSFKLPRPEGALV
                     ASVDASGPAAKAGLKVGDVILKVNGQPIVASGDLPAYIGQRDPGDKVQMEVWRQGHAE
                     TLAATLGNANDKAAKLASNDQAVGKGQLGLTLRPLQPDEAKAAGVPKGQGLLIEDVRG
                     PAAMAGVQGGDVLLAVNGTPVKDVEQVRAAMAHAGKSVALLIERDGDKIFVPVRLG"
     gene            29791..30447
                     /locus_tag="PK518_01765"
     CDS             29791..30447
                     /locus_tag="PK518_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM47667.1"
                     /translation="MRLLLVEDDTMIGEAVRDLLRAEHYAVDWATDGDMADAALAAQS
                     YDLVLLDLGLPRRDGLAVLRSLRARKDRTPVLIATARDGVAQRVEGLDAGADDYVLKP
                     YDLDELLARIRALLRRASGRAEPVYEHQGVALNPGTREATIQGQPVVLSAREWAVLEP
                     LLARPGMVLSRQQLEDKLYGWGDEVSSNAVEVYIHGLRKKLGAALILNVRGLGYMVPK
                     "
     gene            30444..31823
                     /locus_tag="PK518_01770"
     CDS             30444..31823
                     /locus_tag="PK518_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM47668.1"
                     /translation="MNRLRHPASLRVRLLVFLLAAIALTAAVQGLLAYRSALVEADAL
                     FDYQMQQTAYALRAGLPPDARASGNALPEYQNDELIVQVWTNEGLRIFESAVGAALPQ
                     KAVLGFTELQARGISYRVFSLQTRSQVIQVAQDMSARRAMARALALRSLLPLAVMAPL
                     LALAVWWAVSRLLAPVQRVRGQLAQRRPDDLTPVSGAGLPSELQPLVHEFNALLARVQ
                     QAFDAQQHFVADAAHELRSPLAALKLQLQGLQRAPDTATRERAVERLGSGIDRATRLV
                     EQLLALARQEAQIAAGAPPQPVPLAELARQAVLDATPAAQQRGIDLGLAPVPPEAEGA
                     SVPGHAQALAILLRNLLDNAIKYTPRGGRVDVALQADDGHITLLVDDSGPGIAPAERA
                     QVLQRFHRGAQPEGAPASGSGLGLAIADSIARLHGARLQLADAPQLGGLRVSLRLARH
                     TPPRESPHT"
     gene            32183..32542
                     /locus_tag="PK518_01775"
                     /pseudo
     CDS             32183..32542
                     /locus_tag="PK518_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
     gene            32841..33239
                     /locus_tag="PK518_01780"
     CDS             32841..33239
                     /locus_tag="PK518_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRM47669.1"
                     /translation="MDAWCLACRRGVRVSLLVPARSNHRLADIARERALRQLAAAGAQ
                     VWLAPAMVHAKAVLIDDDLALAGSLNLDARSLFLNYEAMAAFYGEQELRWLSQWCARL
                     IATARPYHARQPSWARDIVEGVVRSVGFQL"
     gene            complement(33300..33698)
                     /locus_tag="PK518_01785"
     CDS             complement(33300..33698)
                     /locus_tag="PK518_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRM47670.1"
                     /translation="MSGYLLDTNIISDLIRNPSGAAAQRIAQLGAKGICTSIVVAAEL
                     RYGCAKKGSPRLLAKVQALLETIPVLALDLPADGDYGRIRAELEAAGQPIGGNDLLIA
                     AHACTLRLTLVTNNTREFARIRGLKVENWL"
     gene            complement(33695..33967)
                     /locus_tag="PK518_01790"
     CDS             complement(33695..33967)
                     /locus_tag="PK518_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HRM47671.1"
                     /translation="MTAPTLSSRPKEAKLFRNNRSQAVRIPVEFELPGDRVLIHREGS
                     KLIIEPVLRPTNILELLAEWRQEPPLAPEDQFPAIDDAPVQPEDLP"
     gene            complement(34030..34182)
                     /locus_tag="PK518_01795"
     CDS             complement(34030..34182)
                     /locus_tag="PK518_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47672.1"
                     /translation="MRAKLDEGFLQQFTGRLSLPFAAGANQQVAVKIVDDRGIESLKV
                     VKLPAG"
BASE COUNT         5397 a        11596 c        11823 g         5602 t
ORIGIN      
        1 tctcgaaagc cccgagcttg accatgtcgt gactgccttc gcagttgaag ctcggtgggt
       61 agcccgcgtc gggcgtattg tccttgacct tcagcagttt gccgttgttg ctgaaagtcc
      121 agccgtggaa ggggcaggtg aagctggact tgttgccctt cttgaagcgg cacagaatcg
      181 cgccacggtg cgtgcaggtg ttggccagcg cgttgagctt gccgtccttg ctgcgcgtga
      241 tgaccaccgg ctggcgaccg atgtaaacgg tcaggtagtc gttaatgttg gggatctggc
      301 tctcgtgcgc caagtagacc caattgccct caaaaatgta cttcatctcg agctcaaaca
      361 actgctcgtc ggtgaatgcc gcccgatcca ggcgataaag atggttggcg gggtcatctt
      421 gcagcatcgc atcgagtttg gcgaggctgg tcgagttgtc tgggtatatc gggatcacgc
      481 ggttctccta ttgaatcggt tcaacgtcgg ggtgggtcct gtgctgtgct gggcgcgccg
      541 aggcgccggc acatcagtcg gacaggttgg ggtggcggga atggtcgcca ggcggcggcg
      601 atccatcacg ccacgtgggg cggcggtgcg cgcatcaagg gctgcagggt cattgagctt
      661 gtctcctatg ggtggactta tcgtctcttg gtttcggtct cgccgcatgt tcttgcgacg
      721 ccggtgcgca tttggtctct atggctcgcg gcagtcttgg tgattgcatt tgccgtgcca
      781 actttgcacg actgcaattg ttccactcaa cctcactcga cagcgagtga atcattaggt
      841 gtaaacccga agcacctagt cctggccgca caatcaacca gtgaagcaat tgatgcaaat
      901 aagcgcgccg ggccttgtca cgaatgcgtc atctgatcga tgtagcgggc tttcctgcgg
      961 gtcgcgatca aaggcctctt gcttctgcca cgagggagca cactgcgctt gctcgcgctg
     1021 cgctgcgctt ggcacgttgc gtgctcgcaa tcacgcaagc cgtgcgaaaa attcaaagga
     1081 gacgaggatg ttgatatgcc tagccgaccg cccgggacgg tggccatgaa aaaatccagc
     1141 acgggttccc ctgcggactt gagtctgccg ctgatccacg ccgatcacac tgacattgat
     1201 aaggtctgct tcccgaacct tgtcgatttg atggcgcgcc tgcatttttc gaccagcgac
     1261 ggccgcatct ggctcgacga gcaacgcatg ctgctggtcc acgccaaggc gctgggctcg
     1321 atgcggcgcg agatgatcga atccctgggc acggacgtgg cgcgcggcct gctcactcgc
     1381 atgggttacc aggccggcac ctacgacgcg cagatggcgc gcaaggtgcg ctccaagacg
     1441 tcgttgaagg acatgttcgt cgtcgggccg cagatgcact gcctggaagg catcggcctg
     1501 tccgagccgg tgcgactgga gttcgacgtc gggaagggcg aacactacgg cgagttcctc
     1561 tggacccacc aggtcgagga cgaagagcat gtccgacatt tcccgatcgg caccgaaccg
     1621 tcctgctgga tgcaggtcgg ctatgcatcg ggctactcca cggagttcat gggtcgccag
     1681 atcctctacc gcgaggtcga gtgccagtcg atgggccaga ccgcttgccg catcgttggc
     1741 atgccggtcg aagcatgggg ggatgagggc ttgcaagacc tgcagcacct gatgcccgca
     1801 gcgctgcgca gcgatgcgcc ggaagcggcg gtgtcggcca cacgcaatcc cgtgctgctc
     1861 ggcgctgatg cgacggactc ggcccagcca gtcggcatct caccgggcta caacgccgtg
     1921 ctgcacatgg tgcagcgggt cgcaccgacc aatgccacgg tgctgtttct cggcgaaacc
     1981 ggcgtcggca aggaggtttt cgcgcgcatg ctgcatcgga tgagttcgcg cagccacaag
     2041 cctttcgtcg cggtgaactg cgcggccatt cctgaaacct tgatcgagtc ggagttgttc
     2101 ggcaccgaac gcggtgcctt caccggtgcc acgcagagcc gcgcagggcg cttcgaacgc
     2161 gccgacggcg gcacgctgtt cctcgacgag atcggcacct tgtccgctgg cgcccagggc
     2221 aacctcttgc gtgcgctcca ggagggtgag atcgagcgac ttggcgatac ccagacccgg
     2281 cgcgtcgatg tccgcgtcat tgcagcgacg aacgtcgacc tgcgcgagga ggtggcggcc
     2341 gggcgcttcc gcgaggatct gttcttccgc ctcaatgtct ttccaatccg cgtgacgccc
     2401 gtgcgcgagc ggcgcgagga cattccgctg ttgatgacgc acttcctcgc caagttcaat
     2461 cggctgcatg ggcggcgcct gaccggcttc acacagcgtg cggtcgatgc cgtgatgtcc
     2521 tacgcctggc ccggcaacat ccgcgaactc gaaaacgtca tagaacgcgg cgtcatcctg
     2581 gcagcccaga acacagcgat cgacgctccg cagttgttta ccagcggtga acaattcaac
     2641 cgcaaacagt tcgccgtaag cccggacggg ctactggtcc atgcggatca gactcggctg
     2701 ctgctggagg agccgccagt cgaccccgag atcgaccggg tgtccaagca catcaacaat
     2761 ctgctgaacg gcatcgagga cgattccaag gtatcgctcg acgacatcga gaccttgctg
     2821 ctcaagcagg ccgtgacgcg ttcgcaaggc aatgtcgcaa gggcagcgcg cttgctcggc
     2881 atcacgcgcc cggcgatggt gtaccggctg aagagccgag gtatcctgca ccctggccag
     2941 taaattcaac ccgacagaaa cgacacgcct atgttctccc acctcgaacg gccgtcaggc
     3001 ccgatccccc attggcggca agtgctggcc gactttggcc cgcgtgaagc cgccaatggc
     3061 ttcatcggct gggtcttctc ggccacggca ccggtggcca tcatcctggc ggtcggcaca
     3121 cgcggcggat tgagcgaggc ggagcttgcc tcgtgggtct tcggcgtgtt cttcatcaac
     3181 gggttgatca gcatcttctt cagctggttg tatcggcagc ctctggcctt cgcctggacc
     3241 attcccggca ccgtgcttgt gggccccgca ctgggccacc tcagcttcgc cgaagtgatt
     3301 ggcgcgttct acgtgaccgg agcgttgatg ctggcgctgg gggcaaccgg ctgggtcaag
     3361 cgtgccatgc gcgcggtgcc gatgccgatc gtgatgggaa tggtggcagg cgtgttcctg
     3421 cgcttcgggc tcgatctgat tcgctcgttg cacgaagatg ccctcatcgt ggcgcccatg
     3481 atccttgtct ggctgctgct gacggttttt cccgcgctgg gacgacgcct gccgccgctg
     3541 atcggggcct tgctgatcgg cgcgctggtc gtaattctgg cggggcgatt cgatgcggcc
     3601 agcctcggcg cgttggagtt cgcccgaccc gtgatccatg cgcccgcctg gtcttggggc
     3661 gcgatattcg aactggtcgt gccgctggcg atcaccgtcc tggtggttca gaacggccag
     3721 ggcatggcgg tgctgaaggc cgccggacat gagccgccgg tcgatgccat caccgtcgcc
     3781 tgtggcgtcg gtgccatggc gagcgcggtg gtcggcagcg tcagcacctg cctgaccggg
     3841 ccgacgaatg ccatcatcac ctcttcgggc gaacagtccc ggcactacac cgccgcgatt
     3901 ttctgcgggg tgctcggtgt gatcttcggg ctgctgtccc cggccttcac gcgcttgatg
     3961 ctgcatgcac cgaaagcctt catcatggcg ctcgcgggcc tggccatgct gcgaatcttg
     4021 caggccgcat tcgtcacctc gttcaaggac agattcacat tgggcgcttt ggtctctttc
     4081 ctggtgacgg tggctgatct tggcctgctg aacatcggcg ccgcgttctg ggggctggtg
     4141 gcgggattcg tcatctcatg gttgctggag cggcccgact tcgttgcggt ggcaaatgtc
     4201 tcatgaacac agggattcat tggggtcacg cgggccggca ccgatcgcgc atatcagcat
     4261 gcacggcatt cgaagtcatg taggtgcgca ttgaaaccgg ccgggtgcgt gaaggtcgca
     4321 ccttgaacgg gggtgcctct cagggttcaa tgcaccagat acagcaggcc tgtcgtcatc
     4381 accaaatagc gcgccagctt gcccacggcc atataggcca ggcagggcca gaacggcagg
     4441 cgcagccagc cggccacggc gcatagcggg tcgcccacca cgggcagcca gctcagcagg
     4501 caggtcttgg ggccccagcg gcgcagccag acgcgcgcgc gccgctcggt gcggctgagc
     4561 tcgcgtggcg cgggcaattc ggcgctggcc ggctggccgg cctgccggcg gcgccgcgcc
     4621 gcgtcccagg cgcggtgcgc gccccggccc atccaccagc tcagggcgcc gcccagcgta
     4681 ttgcccagcg tggccaccag gatggcgggc cagaacagct cggggttgag cttgaccagg
     4741 ccgaacaccg ccggctccga gcccaggggc agcagcgtgg ccgagacaaa ggccacgacg
     4801 aataccgtgc tgaggccgaa ctgcggcagc gccagcaggt tcagcagatg ttgcatccag
     4861 gcttccatag cagggctggg agtgtagagg catgggcgcg gcggccctgc cgccatttca
     4921 tttgctgaaa ttttgagcag gtaaaatacc gcccctcacc cgtgctccca tccccaacgc
     4981 ctgacatgca tatcggccat attcccctgg cgaatcgttt gttcgtcgcc cccatggcgg
     5041 gggtgacgga caggccgttt cgccagctgt gcaagcggct gggcgcgggc tatgcggtga
     5101 gcgagatggt cacctcgcgc agggacctgt gggccagcct caagaccagc cggcgcgcca
     5161 accacgaggg cgaacccggc ccgatcgccg tgcagatcgc cggcaccgac gcggccatga
     5221 tggccgaggc ggcggtctac aacgtggagc gcggcgcgca gatcatcgac atcaacatgg
     5281 gctgcccggc caagaaggtg tgcaacaaat gggccggctc ggcgctgatg cagaacgagg
     5341 cgctggcggt ggagatcgcc gcggccgtcg tcgccgcctg cgccccgcac cgggtgccgg
     5401 tgacgctgaa gatgcgcacc ggctggtgcc agcagcagaa gaacgccgtg cagttggcgc
     5461 gccagttcga ggccgtaggc gtgcagatgc tcaccgtgca cggccgcacg cgcgagcagg
     5521 gatacaaggg ccaggcggaa tacgacacca tcgccgccgt gaaggccgcc gtggccgtgc
     5581 cggtggtggc caatggcgac atcaccagcc ccgagaaggc gcgcgcggtg ctggcgcaga
     5641 ccggcgccga tgccatcatg atcggccgcg cggcccaggg gcggccgtgg atcttccgcg
     5701 aggtggcgca tttcctggcc acgggcgagc aactggcccc gccgctggtg gccgaggtgc
     5761 ggcgcctgct gctggagcat ctgcaagacc actacggcct gtacggccag gccagcggcg
     5821 tgcgcagcgc gcgcaagcat atcgcctggt atgtgcgcga cctgcccggc ggcgaggcct
     5881 tcaggcaaca gatcaacacc atggaggact gcgcggcgca gtggcaggcc gtggccgatt
     5941 tcttcgacgc gctgggcgcg cagatggatc ggttgcccct tgccagcgac gacgcggacc
     6001 cgacaacgag ggagtcgatg catgagcaac cacaatattg agcaatgcgt gcgcgaaagc
     6061 ctgcaaagct actttcgcga cctggacggc gagacccccg acggcgtgta cgacatggtc
     6121 gtgcgcctgg tggagaagcc gcttctggag gtggtgatga accaggccga gaacaaccag
     6181 tcccgcgccg ccgaatggct ggggctgaac cgcaacaccc tgcgcaagaa gctgctggat
     6241 cacaagctgc tttgatccaa catacaaaaa aaatagctgt cagcgcttga tggataagcg
     6301 ctggagccga atttcaatcc aaaactctgt aaaccccatg aacgcactcc tctccgtctc
     6361 cgacaagacc ggcatcgtcg aattcgcccg cgccctgcat gccctgggca tcaggctgct
     6421 gtccaccggc ggtaccgccc agctgctggc cagggagggc ctgcccgtca ccgaggtggc
     6481 cgaggtcacg cagttccccg agatgctgga cggccgcgtc aagaccctgc accccaaggt
     6541 acacggcggc ctgctggcgc ggcgcagcct gccggcgcac atggcggctt tgcagcagca
     6601 cggcattgac accatagacc tgctggtggt caacctctac cccttcgagg ccaccgtggc
     6661 caaggccggc tgcacgctgg ccgacgccat cgagaacatc gacatcggcg gcccggccat
     6721 ggtgcgcagc gccgccaaga actggcagga cgtgggcgtg gtcaccgccg ccgaccagta
     6781 cgccggcgtg ctggccgagc tgcaggccca cggtaagctc agcgacaagc tgcgttttgc
     6841 cctgtcggtg gcggcattca accgcatcgc tcagtacgac ggcgccatca gcgactacct
     6901 gtcgtcggtg cagttcgagg aagaaaagct gaacgatgcc tatgtgcccg agcgcagcgc
     6961 cttccccggc cagagcaacg gccatttcat caaggttcag gatctgcgct acggcgagaa
     7021 cagccaccag agcgccgcct ggtaccgcga cctgcacccc gcgcccggct ccatcgctac
     7081 gggcgtgcag ctgcagggca aggaactgtc ctacaacaac atcgccgacg ccgacgcagc
     7141 ctgggaatgc gtgaagagct tcaagcttcc ggcctgcgtc attgtcaagc acgccaaccc
     7201 ctgcggcgtg gccgtgggca ccagcgcgct ggaggcctac agcaaggcct tccagaccga
     7261 ccccaccagc gccttcggcg gcatcatcgc gctcaaccgc gccgtcgatg gcgccgcggc
     7321 gcagaagatt gcgcagcagt tcgtagaggt gctgatggcg cccgacttca cgcccgaggc
     7381 gctcgaagtc ttcaaggcca aggccaatgt gcgcctgatg aagatcgcca ttcccgcccc
     7441 cggcggcgcc acggcctggg agcagggccg caacgccatg gatgccaagc gcatcggctc
     7501 gggcctgctg ctgcagacgg cagacaacca cgagctgcag ctgcccgacg tcaaggtcgt
     7561 caccctcaag cagccgacgc aggaggaaat gcaggatctg atgttcgcct ggaaggtcgc
     7621 caagtacgtc aagagcaacg ccatcgtgtt ctgtaagggt ggcatgacca tgggcgtggg
     7681 tgcggggcag atgtcgcgcc tggattcggc gcgcattgcc agcatcaagg cccaggcggc
     7741 tggtttgagc ctgcagaaca ccgtggtggc gagcgacgcc ttcttcccct tccgcgacgg
     7801 cctggacgtg gtggtggacg cgggcgcgac ctgcgtggcc cagccgggcg gctcgatgcg
     7861 cgaccaggag gtcatagccg cggcgaacga gcgcggcgtg gccatggtct tcaccggcgt
     7921 gcgccacttc cgtcattgaa ttcggtacac ccccctgagc ggctgcgccg cttccccccg
     7981 ctctcgcatt gcgttgcaat gcgggcaggg ggacgcagcc agcgcggcgg ggcggccctt
     8041 gcgcggctgc ccgcgcgctc agcgcccatg gcgcatgttg ctgccctgca tagccaataa
     8101 gcaaaaaggc ccgcaagggc cttttttaat gaaacaaggc tgcagcgctt gtccatcaag
     8161 cgctaacagc tattgttttt atagcttggc gaacacctcg cgcgccagcg ccacggtgtc
     8221 ggcaatgtcc tgcgcgctgt gcgcggcgct gacaaacccg gcctcgtaca gcgccggggc
     8281 gatgtacacg ccgccgtcca gcaggccgtg gaacagctgg ttgaatttgg cgccgtcggt
     8341 cttcagcacc tcggggtagt tctgcggcag ctcggggagg aggaagaagc cgaacatgcc
     8401 gccctggctg tcggcgcaaa acggcacgcc ggcggccttg gcggcgcccg ccagcccatc
     8461 gacgagcgaa cgcgtgcgcg tggccagcgc ctcgtaaaag ccgggcttgg cgatctcgcg
     8521 cagcgtcgcc aggccgcagg ccgtggccac cgggttgcca ctcaaggtgc ccgcctggta
     8581 caccgggccc agcggcgcca gctgctccat gatggcgcgc ggcccgccaa aggccgccag
     8641 cggcatgccg ccgcctatca ccttgcccag caccgtgagg tcgggtttga aaccgggaag
     8701 ctgctgcgca tacaggccct gcgcactgcc cagggccacg cgaaatccgg tcatgacctc
     8761 gtcgatgacc agcagggcgc cgttctgcgt gcagagttcg cgtgcgcggc gcatgaaggc
     8821 ggtggaagcg cgcacgaagt tcatgttgcc ggcaatcggc tccatgatca cgcaggcgac
     8881 ctccttgccg tgcagcgcga aggcctcttc gagctgggcc acgtcgttgt attcgagcac
     8941 cagggtgtgc tgcaccacct cgggcggcac gccggcgctg gtggcatggc caaaggtggc
     9001 caggcccgag ccggccttga ccaggagtga atcggcgtgg ccgtggtagc agccgttgaa
     9061 cttgatgatc ttgctgcgcc cggtggcgcc gcgcgccagg cggatggcgc tcatgccggc
     9121 ttcggtgccc gagctgacca ggcggatcat ctccatcgac ggcacatggc ggatgatttc
     9181 ttccgccagt tcgacctcgc gctcggtggg ggcgccaaaa ctgaagcctt ccagggcggc
     9241 cttctgcacg gcctccagca cggccgggtg gccgtggccc aggatcatcg ggccccagga
     9301 gccgatgtag tcgatgtact tcttgccgtc ggcgtcccac atgtagggcc cttgggcgcg
     9361 ggcgatgaag cgcggcgtgc cgcctacggc gcgaaaggcg cgcacgggcg agttcacgcc
     9421 gccggggatc agcgccttgg cgcgttcgaa cagggtttgg ttgtggtcag tgctgtgtgt
     9481 catggggtgg aaaatcaaaa tccgccgcgg tgggcgcggg ctcttcacta ttgtcgtcct
     9541 catcgggctc ggcccagaac atccggtcgg gcagcacatg gcccatgccg ggcctgaagc
     9601 catgctccag gcagccgtcg aggtaggtca gggcctcgct cgtggcctcg cccaggtcgt
     9661 tgccgctggc caccagtgcg gccagcgtgg ccgacagggt gtcgccggcg ccgatgaagg
     9721 tggcctcgaa gcgctcgaag cgcccgctgc ccagcacggt ctgggccgag gtgagggtgt
     9781 tctcgatatg ctgctcggcc gcgggaatgc cggtcaccag caggtagggc acgccgctgt
     9841 cggcgaccgc catggccagg tcgcgcgccg tgggggcgcg ctcgctgctc cagtccggca
     9901 gcagccagcg ccacagcgtg ctgtggttgc ccaccaacac cgaggtctgg ggcagcagca
     9961 actccaggaa ggcgtcctgg tattggtcta tcaggtcgtc gtgccaccag gacaggtcgg
    10021 gcatgtaggc gactaccggg atttcgggat agtcggtggc aatctcggcg atggcgctca
    10081 ggttctcggg gctgccgaca aagcccacct tgatggcctg cacggccatg tcctcgagaa
    10141 cggcgcgcgc ctggtcggcc acggcatcgt cgtcaaaggc gcagtggtcg tggatctcgg
    10201 cgctgtcgcg gatataggca ccggtcacca ccggcgccgg gtgcccgccc accgaggcca
    10261 ccgccgcgat gtcggccgcc aggccgcccg cgccgcaggg gtcgttggca ttgaaggcca
    10321 gcacgcagat ggcgggcagc acggcatcgt catcggcggg ggctggattg tcttgggagg
    10381 aggaagtcat cgtgctcatg gtcgggtgcc agcgtaaccg gtggcgtgaa aacagcaccc
    10441 cgcagtcatg actagataca atcgttgcat tctatgtggg tgccccttaa gctgtgaccg
    10501 acctgaagac ctggatgtgc gtggtgtgcg gctggatcta tgacgaggct gagggctgtc
    10561 ccgaggacgg catagcgcca ggcacccgct gggcggacat cccggcgagc tggacatgcc
    10621 cggaatgtgg ggccggcaag caggatttcg agatggtcga aatatgagat ggcgggcgcg
    10681 gcgatggccg gcctggtgtt ttgaccgcct aagaggagca atgggaaatt ggctatgacg
    10741 acaagcgata ccactttcag agtactcgtg gtggacgaca gcaacaccat tcggcgcagt
    10801 gccgagattt ttttgaagca gggcggtcac gaggtgttgc tggccgacga tggcttcgac
    10861 gccttgtcca aggtcaatga ttaccagccc cagctgatct tctgcgacat cctgatgccc
    10921 aagctcgatg gctaccagac ctgcgccatc atcaagcgca acgcgcgctt ttccgatacg
    10981 ccggtggtca tgctgtcctc caaggacggg gtgttcgaca aggcgcgcgg gcgcatggtc
    11041 ggctgccagg aatacctcac caaacccttc accaaagacc agctgctgct ggccgtgcag
    11101 caatttgcca atgcgcaaca aggagtgatg tgatggctat ccgcaaagtg ctggtcgtgg
    11161 acgactcgaa gaccgagttg ctgtacctga ccgacctgct gcaaaaaaag ggtatgcagg
    11221 tgcgcaccgc cgaaaacggc gaagaggcct tgcgccgcct ggccgaggac aagccggatc
    11281 tgatcctgat ggacgtggtc atgcccggcc agaacggctt ccagctcacg cgctccatca
    11341 cgcgcgaccc gctgtatgcc gatgtgccgg tgatcatgtg caccagcaag aaccaggaaa
    11401 cagaccgcgt ctggggcatg cgccagggcg ctcgcggcta tatcaccaag ccggtcgatc
    11461 ctgcggagct gcaggccaag atcgacgcgc tgaactgaca ccatggccaa tcgcgaagcc
    11521 ctgaaggagc tgcagaccag gctggccgcg cgcctgcagg ccgccaaggc cgagggcgtg
    11581 gccgtcgcat cctggctggc cgtggagtct ggcgcgcagc atttcctgtt gcccctggcg
    11641 cagtcgggcg agatcttttc ctggaccggc gtgcagcccg tcccctatac ccaaccgtgg
    11701 tttttgggcg tggccaattt gcgcggcacg ctcaccgggg tggtggatct ggcgggcctg
    11761 ttgggcggct cgcctgcgcg cccggagcag gcgctgttcg aatccagcct gctggccttg
    11821 aacgccgccc tggaggtgaa tgccgcgctg ttggtggaca ggctggtggg gctgcgcggc
    11881 gctcaggcgt ttgtcgcctc cgagccgccg gccgccgagg ccgccgctta ttggggcaac
    11941 acctatgtcg atgcacaggg cacgcgctgg caggagctga acctgcaggc cttggcgcag
    12001 cacccgacgt ttctcagcat cagcgcatga atttttgtga ggcagaacat gtccgtcgtc
    12061 aatccactgg aaaaactgtt tttgcgcaag cctgtccaag gcgatgctga tttgggccag
    12121 ccggacatca tccaggactc gatctccgcc ggtgttctgt cacaggccta ccaggccgac
    12181 ggcatgccca gcgtcctcgg cgactatggc gacaccgtgc gcaagcccga gcccggcgtg
    12241 gctgaacaag agcagatcag cctgcccctg ctcggcagtg ccagcaccgg gacgcaccag
    12301 cggcgcctgc tgatcctgct gggcgtgggc ctggtgctgc tggcgctgat tgccggctgg
    12361 gcgctgcagc agtcaagccg gtctgccaag cagctggccg ccatggggca gtcgctgatg
    12421 cagtcgcaac gcttggccaa gtcggtgtcg caggcactgg cagggcagcc gcaggccatt
    12481 gccgacatga aggacagtgc cgacgtgctg gcgcgcaatc tgcgtgcgct caacgccggc
    12541 gatgccgaac tgggtgtgca ggcgctgggc gaatccttca agcccgagct cgaagcgatg
    12601 aacccgctgg tggagcgggc cgaacgcaat gccggcctgc tgttggggca aaaggccatc
    12661 ctgacccagg tgggtgaggc cttgcgcgcc atcaaccagc agtctccggc cctgctggag
    12721 agcgccgagg gcattgccgc cctgaagctg cagaacgccg cgcctgcggc cgacatttcg
    12781 gccgccggcc agctggtgat gttgacgcag cgcatcggca agtcagccaa cgagttccag
    12841 gccgccgagg gtgtgaaccc cgagtcggtg ttcctgctcg gcaaggactt gaattccttc
    12901 aaggagatcg cccagagcct gctggccggc acgcgcgacg cgcagacgcg cgagcagctg
    12961 caggcattga tcacgctctt tgaccagaca cgcacgcagg ccggcgccat cctgggcaac
    13021 ctgcaaggcc tggcggcggc acgccaggcg caggcggcca tcgtggccga cagcgagccg
    13081 ctgcgccgcc agctcgaaac cctgcaggac aagctgtccg cgcagtccgg cgtggggccg
    13141 gggcagattg ccgccctggt cgtggcgggc ctgttcgtgc tgctgtgcgg cgtgggcgtc
    13201 tcgcgcgtgc agctgctcga cagccgcggc cgccaggtgg ccgccgaggc gctgcagatc
    13261 gatgccaagc gccaggagca ggaggccaag cgcgtcaacg acgccaacca ggccgccatc
    13321 ctgcgcctaa tgaacgagct gcagtcggtg gccgagggcg atttgacgca ggaggccacg
    13381 gtgaccgagg acatcaccgg cgccattgca gactcggtga actacaccgt ggaggagctg
    13441 cgcctgctgg tgggcagcgt gcagaacacg gcggcgcgcg tggcgcagac cacggcgcag
    13501 gtggacagca cttcgaccga gctgctggcg gcttcgaccg agcagctgcg cgagatccgc
    13561 gagaccggcc gctcggtgct ggacatggct tcgcgcatca accaggtgtc gtcgcaggcg
    13621 caggaatcgg ccgccgtggc gcgccagtcg ctgcaggcgg ctgaccaggg cctgcgcgcg
    13681 gtgcagaaca ccatcggcgg catgaacgcc atccgtgacc agatccagga cacctccaag
    13741 cgcatcaagc gcctgggcga atcgtcgcag gagatcggcg agatcaccga gctgatttcc
    13801 gacattaccg agcagaccaa cgtgctggcg ctcaacgccg ccatccaggc cgcgtcggcg
    13861 ggcgaggccg gccgcggctt ctcggtggtg gccgaggaag tgcagcgcct ggccgagcgc
    13921 tcggccgacg ccacgcgcca gatctcggcg ctggtgaagg ccattcagac cgacacccag
    13981 gatgcggtgg ccgccatgga acgctccacc cagggcgtgg tcgagggcgc gcgactgtcc
    14041 gacagcgccg gcaccgcact cagcgaaatt gatagcgtct cgcgcaggct ggctgagctg
    14101 attgagcaga tttcatcctc tgcttctaaa gaagcggagc tggccaacgg cgtggccgac
    14161 aacatccagc acatctttgc cgtgaccgag cagacgggtg agggcacgcg cgccacggcg
    14221 cagcaggtgc gcgagctctc gcgcatggcc gacgagctgc gccagtccgt ggcccgattc
    14281 aagatcgctt gacgccatct catcaacgca ttggctcaag cagccgggga cgcatccatg
    14341 tcatctcttg acgccgcaac cgcaacgggc gccgacggga ctcagaacga gcaggatctc
    14401 gggccgctgg catgggtgct cgacgagctg cgcaaatccc tggagggtgt tgccaagtcc
    14461 atgcgccgct ttgtgcgcga cgccgagatg gcgcgccagt cagacctggc ggcgctcgat
    14521 gccgctgcac tgcgcattgc gcgccagcag ctgcaccagg ccggtggcgc gctgacgatg
    14581 gtgggcatgg agtcgccggc actgctgctg cgcgcgatgg aggccgccgt gcagcgcttc
    14641 gtgcaccgcc ccgagctgtg cagcgacgat gccgcccgca ccatagagcg cgccagcttt
    14701 gcgctgatcg aatacctgga atgcgtgctg gcgggtcagc gcatttcacc ggtcgccttg
    14761 tttccgcagt accgcgacgt gcaggccctg gctggcgtcg atcgcgtgca tccggccgac
    14821 ctgtggccca tgaacgcgcc cgtgcaggcc ccggccttgc ccgtgggcgt ggccccgctg
    14881 ccctatggcg ccgaggcgcg ctcgcgcctg gacagcgccg tgctgcgcat cgtcaagaac
    14941 gccgacccgg gtgcggccgc agacatgcgg gccatctccc agggttttgc cgcggcacag
    15001 gcagagcccc aggtgcgcat gttctggacg gtctgcgccg gcttcttcga ggccctggcg
    15061 cagcaggcgc tcaagcccga tctgtacgcc aagcgcgtcg catcgcgcgt gctcatgcag
    15121 tacgccacgc tggccaaggg cgagcacggc atcactgagc gtctgttgca ggatctgctg
    15181 tttttctgtg cgcaggcggc gcccgagccg acggccgcgg ccgccacgcc ggcactgcag
    15241 gccgtgcgcc agagctttgg tctgcagcgc cacccgccgg tggactacga acggccacgc
    15301 tttggcctgt tcgacccggc cctgctggca caggcacgca agcgcattgc cgcggccacc
    15361 gaaacctggt cggccctggc cggcggtgac cgcaacaaac tcaagccggc ggtggatcag
    15421 ttcagcctgg tgtgcgattc gctgcgcaag ctgtgccccg aaagcgagcg cctggcgcag
    15481 gcattgacgc atgtgctgga cgtcacgctg cgcagcggtg aacccccgcc agcggccctg
    15541 gccatggagg tggccactgc cgtgctgtac ctgcaggcct cgttcgagga gctcgacgcc
    15601 tcccgtgacg ccatgggcca gcgcgccgag cgcctggccg agcgcctgga cagcgttgtc
    15661 gcgggcggcc agtcgcaacc gctggagtcc tggatggagg agctgtaccg ccgtgtcagc
    15721 gaccagcaga ccatgggcag cgtggtcggt gagctgcgcg ccaccatggc cgaggccgaa
    15781 aagtccatgg atcagttctt ccgccagccc gacgatctgg cgcccctggc cggcgtgcct
    15841 ggcggcctgc agcaaatgcg cggcgtgctg tcgctgctgg gcctggacca ggcatcgctg
    15901 gccgtggtgc gcatgcgcga catggtggag cggctgctgg tgggcgaggt ggcgcccgag
    15961 gagcgacagc aggtattcga gaagctgggt aacagcctgg gcgccctggg ttttctcatc
    16021 gacatgctga gctatcagcg tgccatggcg cgcaagctgt ttgtctatga ccaggagctg
    16081 ggagagctgc gcatcgtcat gggacgcgcg cccgtggggc agccggagcc gagcgcagcc
    16141 gcgccagccc aggcgcagtc gcccgcgcct gagtcagagc cagaacccgc gccgcaagca
    16201 gtggtgccgc agcctgccga gccagccccg ttggatcttg ccgtccatgc tgcgccggtg
    16261 ttgccgcagc ccgaggtcgc gccgaccccg ctggtccctg tggccgcggt gacactgccc
    16321 gatgcgggcg ccgacgacga tgccgacgac gatgcggagc ttctggatat cttcctggag
    16381 gaggcgcgcg aagtcgtggc caccggcctg gcggccgtgc aggcattgca cgccgaaccg
    16441 ggcgacttga gcgagcagac cacgctgcgc cgcgccttcc acaccctcaa gggcagcgcg
    16501 cgcatggtgg gcctggccga gttcggtgag gcagcctggg ccatggagca aacgctcaat
    16561 gcctggctgg ccgaggaaaa gcccatgtcg gcgccgctgc tgcagctgtc cgacgaggcg
    16621 ctgcaggcct ttggccgctg ggccgatgac atcgaggccg gcgtcgccgg tcactggcag
    16681 gcgctgccct ttggcgccgc cgccacggcc atgcgcgagc agggcaccct gctgccgctg
    16741 acagccgcca gcgaattcct gatggaggtc gaacccagtc cggagccggc gctgccgccg
    16801 gtcgtggccg atgaggccga gtcggcaccg tcggccaatg ccgagcccgg cctggaggac
    16861 catggcgatg ggccgcagca ggattttgcc gacacctgca tcgagctgcc ggactttgcg
    16921 gcgtcggtgc cgcccgccgt cgaggtgacc gaaaccgtac tgctggacga actggatttc
    16981 gcggtcttcg agcaggcgct gcaggcccag gccgacgatg tgaccgagcc gctgcccgag
    17041 tccacggggt tggctgttgc cgacacggcc gccgaagagg tgttgcacca gcccagtccg
    17101 ttggacgatg gctttgctct tgacattccg gtggatgcgg tcgaaggcgt gggccagcca
    17161 ggcgaggccg agccgcccga ggccgtggcc gctggcgatg aggccgatga atccgaggcg
    17221 cttgaagtct tgccctccac ggacgaggcg atcaaggtga ttgagcacct gcgcatcggc
    17281 atcccgctgt acaacgtcta tctgaacgag gccgacgaat ggtcgcgccg cctggccacc
    17341 gagctgcagg aatggtcgct ggaattgcac cggcccgtgc cggaggcggc cgtggcgctg
    17401 gcgcattcgc tggccggcag ctcggccacc gtgggctttg ccgccctgtc ggatctggcg
    17461 cgccagctgg agcatgcgct gcagcatgtg cagctgctgc cgcatggcct ggccgagcat
    17521 gcccaggcct gcaatgcagc ggccgacgac attcgtcgcc tgctgcacca gtttgccgcc
    17581 ggttttctca aggagccgaa tccttcggtg cgcgagcgcc tggcggccgt cttgcgcatc
    17641 gaggtcagca gcggcgcggc gccgctggag gccatggcgc agagtcccga tacgctggag
    17701 ctggagtttg gcgacctggc catgcaggac gaggtggccg acctgccgac cgaaccgtcc
    17761 gggcagacca gcgagccagc ggatgaccag ccagatgccg cagggcagga cttggaggcc
    17821 ggcttcgagc atgaccgcgc catcgatgaa gcgattgcgc gtgcggtcgc cgccgttgac
    17881 gatatcgacg atgacatcga cgcactcgac gtcatcgacc cggacctgtt ccccatcttc
    17941 gaggaagagg cggccgagtt gctgccggcc ctgggctcgg cgctgcgcca atgggcggcc
    18001 cgcccggaaa acctgagtgc ccgcaacgag ctgctgcgcg cgttgcacac cttgaaaggc
    18061 agtgcccgcc tggccggcgc catgcgcctg ggcgagatgg cgcaccgcat ggaaaccgcc
    18121 gtcgagcaga tcggcaccaa cggcgtcacc acggcggcgg tggagcccct gctggccagc
    18181 cttgatgtcc tgcaggcgga tcttgacgtc ctgcgcacga ttggcgcaca accgctgtcc
    18241 gaacccatgg tggtgtccgc gcccgaggcc gccacgccgg cgtctgctgc cgctgtgccg
    18301 cagctgccgg cggccgccgc ccccgtcgcc gcgccgttgt cctggcgcgt gccggctagc
    18361 gccagtgcca ccgtgccggc acagcgcgcc gccacgcagt cggtgcgtgt gcgtgcgcag
    18421 ctgctcgacc gcctggtgaa ccaggccggc gaggtgatga tctcgcgctc gcgcctggac
    18481 gcgcgcctgg ggcagatgcg cgcgtcgctc gccgacctga cgggcaatct ggagcgcctg
    18541 cgccagcagc tgcgcgacat cgaggtgcag gccgagacgc agatgcagtc gcggctggcc
    18601 ctgtccaagg atgtggcggc cgggttcgac ccgctggaat tcgaccgctt tacccgcgtg
    18661 caggaactca cgcgcatgat ggccgagtcg gtgaacgacg tggccacggt gcagcgcaac
    18721 ctgcagcgca gcatggaagg ggcggaggac gacctgatcg cccagggccg ccaggcgcgc
    18781 gagctgcagc gcgacctgct acgcacgcgc atggtggagt tcgagagcat tgccgagcgc
    18841 ctgtacgccg tggtgcgcca ggcatccaag gaaaccggca agcagatcaa gctggacatt
    18901 tccggcgggt cgatcgagat ggatcgcggc gtgctcgatc gcatggcgcc ggcgttcgag
    18961 cacctgctgc gcaattgcgc cggccatggc atagaggcgc ccgagacgcg cgtggccgcg
    19021 ggcaagccgg ccgtgggcac catcaccatc gccctgcgcc acgagggcaa cgacgtttcg
    19081 gtcgagttca gtgacgatgg cgccggcctc gacctggagc gcatccgcgc ccgggccgtg
    19141 gcccaggggc tgattgcgcc ggacgaaccc atcaccgcca cgcaggccgc gaacctgatc
    19201 ttcaggcccg gcttcaccac cgccagcgaa gtcaccggcc tgtctggccg cggcataggc
    19261 atggatgtgg tgcgcgccga gctcaatgcc ctgggcgggc gtatcgagac gcagaccgag
    19321 gccggcaagg gttcgtcgtt ccgcatgctg ctgccgttga ccacggccgt gacccaggtg
    19381 gtcatgctgc gcgccggcgg gctgaccctg ggcgtgccgg ccaacctggt ggaggtggtg
    19441 cgccgcagcc cggtggccca ggtggacgcc gcctatgcca gccagtggtt tgccgaagga
    19501 tccgagtcgc tgccgttcta ctgggccggc gccttgatgc aatcggcacc gcgcagcacc
    19561 gaagtggcgg gcaagacccg gccggtgatc attctgcgca gcgcctcgca gcgcctggcc
    19621 ctgcatgtgg acgaggtgct gggcaatcag gaagtggtgg tgaagaacct ggggccgcag
    19681 ctgtcgcgcc tgccgggggt ggcgggcatg tcggtgctgg cctcgggggc ggtggtgctg
    19741 atctacaacc ccgtggccct ggccacggtg tatggcgagc aggcgcgcgc ggccatgctg
    19801 gcgcccgcgg cgcaggatgc gggcatggtg gcggacatca cgcgtgccgg catgtcggcg
    19861 ccagcgtcgt ccgtggcgca gctcgatcag gtgccgctgg tgctggtggt ggacgactcc
    19921 atcaccgtgc gccgcgtcac gcagcgcctg ctgcagcgcg agggttaccg cgtggcgctg
    19981 gcgaccgacg gcctgcaggc gctggcgcgg ctgcagcagg agcgcccggc ggtggtgctg
    20041 tcggacatcg aaatgccgcg catggacggc ttcgacctgg cgcgcaacat ccgcgcggac
    20101 gcggcgctgg ccgatctgcc catcatcatg atcacctcgc gcatcgcgca aaagcaccgc
    20161 gaccatgccg cgcagctggg cgtcaaccat tacctgggca aaccctattc cgacgaggaa
    20221 ctgctgagcc tcgtgggcca ctatgcccag ctgacggtcg cgcaaggcgg ttgaccggag
    20281 ggggcggtgc ccccagggtc cggcccggct ttgcgccggc ctggccctgg ctctggccta
    20341 aaattggccc atgtctgcca cttccgcgcc catgaacctg acgcaccatt tcctgattgc
    20401 catgccgggc atggaggatg cgtcctttgc gcgcagcgtg gtctatctgt gcgagcacag
    20461 cgagcgcggc gccctcggcc tgatcatcaa caagccgacc gacatcagcc tggagggcct
    20521 gttcgagaag gtggatctga cgctggggcg cgcagacctc acgcaccagc cggtgttcct
    20581 gggcggcccg gtgcagaccg aacgcggttt cgtgctgcac gacccgatgc gcggcacgcc
    20641 ccagggagca tcgacaccag acgcggacga agacgattcg ccctatgcct ccaccatgag
    20701 cattcccggt ggcctggaga tgaccacctc caaggatgtg ctggaggcgc tgtcgcacgg
    20761 tgccggcccg cggcgcgtgc tggtcacgct gggctactcg gcctggggcg aggggcagct
    20821 ggaatccgag ctggccgaga acagctggct caccgtggat gccgacgctg ccatcatctt
    20881 cgacaccccc gtgggcgagc gctacgagcg cgccctgggc ctgctgggcc tgcagtcctg
    20941 gatgctgtcg cgcgacgcgg ggcacgcatg agcggcgcgc ctgccccggc cgccgttccc
    21001 gcgcatttcc aatcctttct ggcctttgat ttcggactca agcgcaccgg agtggcggtg
    21061 ggcacgcgca tgctgcgcac ggcgacgccg cagggcacga ttcgcgccga gggcgatgcc
    21121 cgctttgcac ccatagcgca gcgcatcgcc gaatggcagc ccgacgcgct ggtggtgggc
    21181 gtgccgtttc atccggacgg cgcgccgcac gagaacacgg cgcgcgcgca gaagtttgca
    21241 cgccagctgc gcgggcgctt tggcctgcag gtgtttgagg tggacgagcg ctacagcacc
    21301 accgaggcgc aggccgccgg cgcgcttgac gcggacgcgg cctcggcctg catcatcctg
    21361 gaacaattct tgaggagtct tgcatgaaca ccaccacagg cagtgcgctg ctgctggatg
    21421 ccgaggcctt gtatggcgag ctgctgcgcg gcgtgcgcgc cctgctgcag ccggacacgc
    21481 gcctggcggg catcacttcg ggcggcgcct ggctggtcga gcgcctgcag cgcgacctgg
    21541 gcctggcggg caagcccggc gtgctgtcct ctgccctgca ccgggacgac ttcgcccagc
    21601 gcggcatggc cgccagtgcg cagacgcagc ttgatttcga tgtcaacggc gccgacatcc
    21661 tggtgcttga cgacgtgctc tacaccgggc gcaccgtgcg cgccgtgctc aacgagctgt
    21721 tcgactatgg ccgcccggcg cgcgtgcgcc tggccgtgct ggtcgatcgc ggcgggcgcc
    21781 agctgccgat cgccgccgat tttgccgccg cccgcgtggc cctgcccgcc agccagatcc
    21841 tggcgctggc gagcggagcg gacggcagtt tccaattcgc agtcaaagag gcctgaaccg
    21901 tgctgtacaa gcgcaacccc caactcaaca agaatggcga gctgatccat ctgctgtcca
    21961 tcgagggcct gcccagggac atcgtcacgc atatcctgga cacggccgcc aacttcacca
    22021 gcgtcaacga ccgcgaggtg aagaaggtgc cgctcctgcg cggcaagagc gtgttcaacc
    22081 tgttcttcga gaacagcacg cgcacgcgca ccaccttcga catcgccgcc aagcgcctgt
    22141 cggccgacgt gttcaacctg gacatcgcgc gcagctccac cgccaagggc gagaccctgc
    22201 tggacaccat agacaacctg acggccatgg ccgccgacat cttcgtcgtg cgccacagcg
    22261 agtcgggcgc gccctacctg atcgcccagc atgtgggccc gcatgtgcat gtggtgaacg
    22321 ccggcgacgg ccgccattcg caccctacgc agggcctgct ggacatgtac accatacgcc
    22381 actacaagaa ggatttttcc aacctgacgg tggccatcgt gggcgatgtg ctgcattcgc
    22441 gcgtggcgcg ctcggacatc cacgccctga ccacgctggg tgcggccgag gtgcgcgtgg
    22501 tcggcccgcg caccctggtt ccgtcggacc tgtcacacat gggcgtgcgc gtgttccaca
    22561 gcctggagga gggcatcaag ggctgcgacg tggtgatcac gctgcgcctg cagaacgagc
    22621 gcatgagcgg tgcgttgctg ccctccagcc aggagtattt caagagcttc ggcctcacgc
    22681 ccgaaaagct gcggctggcc aagcccgacg ccatcgtcat gcaccctggc cccatcaacc
    22741 gcggggtgga gatagcgtcc gccgtggtcg atgggccgca ggcggtgatc ctgccgcagg
    22801 tcaccttcgg catcgcggtg cgcatggcgg tgatgtccat cgtcgctgga aacgacgctt
    22861 gattactatc atattaatag ctgttggcgc tttatggatg agcgctagag ccttgtttcg
    22921 cttgaaacca tgaaaatact cctacagaac ggccgtgtgc tcgaccccgc cagcgggttg
    22981 gacaaggtct gccacctggc gctggccgcg ggccgcatcg tgggcgtgga tcgccagccg
    23041 gcggacttca cgcccaaccg cgtcatcgac gccagcggct gcctggtgct gcccggcctg
    23101 gtggatctgg cggcgcgcct gcgcgagccg ggccacgagc acgagggcat gctcgaatcc
    23161 gaaatggccg cggccgtggc cggcggcgtg accagcctgg tctgcccgcc cgacaccgac
    23221 ccggtgctcg acgagcctgg cctggtggag atgctcaagt tccgcgccga gaagctgcac
    23281 caggcgcgat tgttcccgct gggcgcgctc acgcgcggcc tggccggcga ggtgctgacc
    23341 gagatggccg agctcaccga atcgggctgc gtgggcttca gccaggccga tgtggccctg
    23401 accagcaccc aggtgctgca gcgcgcgctg cagtacgccg ccacctacgg ctacaccgtc
    23461 tggctgcgcc cgcaggagct gtatctgggc aagggcgtgg ccgccagcgg ggcgctggcc
    23521 acgcgcatgg gcctgtcggg cgtgccggtg gcggccgaga cgattgcgct gcacaccatc
    23581 ttcgagctgc aaaagaccac cggcgcgcgc gtacacctgt gccgcctgtc gagtagcgcc
    23641 ggggtggagc tggtgcgcca agccaaggcc gccggcctgc ccgtgacctg cgacgtgagc
    23701 atcaactccc tcatgctgac cgacatggac ataggctact tcgacagccg cgcgcgcctc
    23761 acgccgccgc tgcgccagca acgcgatcgc gacgcgctgg ccgcggccct ggccgacggc
    23821 accgtggacg ccctggtctc cgaccacacg ccggtggacg aggacgccaa ggccgtgccc
    23881 tttgccgagg ccgagcccgg cgccacgggc ctggagctgc tgctgtccgt ggccctgaaa
    23941 tggtcgcgtg acgccggcgt gcccctggcg cgcgccgtgg ccgtggtcac cagcgagccg
    24001 gcgcgcgtgc tgggcagcgc cctgggcacg ctgcaggcca gcgtcggccg catcgtggag
    24061 ggcggcgtgg ctgatctgtg cgtgctcgat ccgagcgctg actggacggt gcaggccaag
    24121 gccctggtca gccagggcaa gcacacgccg ttttcgtcct acgagctgcc gggccgcgtg
    24181 cgctggacgc tggtcggggg ccaggtcgcc ttcgaccgcg gctgaaccac ggcgtgccgc
    24241 gtcacctgcg cgccggctgg cgcctgctgc gcctgctggt gcatgcgctg tgggggctgt
    24301 ggctggtggc ctggcgcttt ccacgcctgt cgtccgacca gcagcaggcg cgcgtgcagg
    24361 cctggtcgct gggcctgctg acctgcgccg gcatcacgct gcagctgcgc ggccggccgc
    24421 agcgcgcggg gccggtgctg ctggtggcca accaccattc ctggctggac attcccgtac
    24481 tacatgcggc gcgctactgc cgcttcatct ccaagtccga cctgcaggac tggcccatcg
    24541 tcggcaccct ggccacggcc gcgggcacgc tctacatccg gcgcgagtcc cgccgtgacg
    24601 cgctgcgcat ggtgaccagc atgcgcgacg cgctgctcgc aggcgaggtg ctggcggtgt
    24661 tccccgaggg ccagaccggc gacgggcgcg cgttgctgcc ctttcacgcc aatctgctgc
    24721 aggccgcgct ggacgcgggc gccccggtgc agcccgtggg cctgcgtttt ctcgatggca
    24781 ttggcggcgc caccagcgat gcgcccagct ttgtcggcga cgagagcttt ctgggctcgg
    24841 tctggcgcac cttgaaggcg ccgggtatca ccgccgtggt gcactttggt gcgcccgagc
    24901 gggccctggg gcgcgatcgg cgtgcctgga gccaggcgct gcatgcagcg gtcgatgcgc
    24961 tgcggcacgg ctgaagccgc tcctaccggc atgccatcgc gttcaggcgc gggcgaaggt
    25021 caccacatga tcgacctggg cacgtatgcc aatccattcg cccatggcat ggtcatggtg
    25081 gctgggcaca tgggccacca cggtgaggcc gccctccagc tccagtgtgt agagaaactc
    25141 cgagccgcga aacgccttgc gcacgatgcg cgcgcgcacc ggggcggcgt cgtcgtgcac
    25201 cacgtcgtcg gcgcgcagca gcacgtcgca ttcgccgtgc gggtagctcg acggcagcag
    25261 gcagccgtcg atgtccgtca ggtcgcccag cgcggtgcgc gccaccacct ggccgccgtg
    25321 ccgcaccagg gtggcggggg cgaacacgcc atggccaatg aagtcggcca cgaagcgcgt
    25381 ggccgggcgg tggtagagcg cataggcatc gtcccactgg tgcaggtgac cgccgtccat
    25441 cacgccgatg cgatcgccga tggcaaaggc ctccagctgg tcgtgcgtga cgaacagggc
    25501 cgtggcgttg gcggttttca gaatgccgcg cacctcatgc gccaggcgct cgcgcagatc
    25561 gacgtcgagg ttggaaaacg gctcgtccag cagcatcagg cgtgggcgcg gcgccagtgc
    25621 ccgcgccagg gccacgcgct gctgctggcc gcccgagagt tcatgcggaa agcgtgcctc
    25681 gctgccttcc aggcccacca gggccaggca ttcgcgcacg cgctgctgct gctcgccgcg
    25741 cggcagctcg tggatgccga aggccacgtt gcgccctacg gaaaggtgcg ggaacagggc
    25801 atagtcctgg aacaccatgc cgatgcgccg ctgctcgggc gccacgctgc gccccggggc
    25861 acccaccagc tcaccgctta agcggatgct gccggcagcg acggcctcca ggccggccac
    25921 ggcgcgcagc agcgtggtct tgccgcagcc cgaggggccg atcagcacgc cgatctcgcc
    25981 cgcggccagg cccagggtca cgccatgcac ggcgggggtc tggcggctgc catagcgcac
    26041 atccagctga gagacttcga gaaacatgtg ccgattgtaa aagtcgcttg cgtagaatcg
    26101 ttcccatttg catctcaacc gccgagccct ctgcggccgc gcgccctcac ttgccacccg
    26161 tcccacgcgc cctgcgcgcc atcgctcccc tcatgctggc cctgctgctg gccctgcccg
    26221 tgctggcggt gctgggcgcc tggctgccgc tggggcaggg cgatgcgcag tccggcgcca
    26281 tcctgcgcga gatggccgcc accgtgctgc cggactacct gtggaccacg ctgtggctga
    26341 gcctggccgt ggccattggg gccgccctgg tgggcacggc cagcgctgcg gcagtcacgc
    26401 tgttcgactt tgccggccgg cgccagctgg aatggctgct gctgctgccc ctggccatgc
    26461 cggcctatgt cacggcctat gcctataccg actttctgca gttcagtggc ccgctgcagg
    26521 tctggctgcg tgaggctttc ggcctgcagg ggcggttgtt gcccgaggtg cgcagcctgg
    26581 gcggcgcgat ctgggtgttc atcttctcgc tctatcccta tgtctacctg ctggcgcgca
    26641 cggccctggg cgagcgcgcc gcgcacctga tggaggcggc gcgcctgctc ggcgccgcgc
    26701 ccgcgcggcg cctgcgcagc gtggccctgc cgctggcacg cccggccgtg gccgcgggcg
    26761 tggccctggt gctgatggag acgctggccg actttggcgt ggcctcctac ttcggcatac
    26821 agaccttcac caccggcatc tacaaggcct ggctgtccat ggacaaccgc ctggccgcgg
    26881 cgcagctggc caccttcctg ctcgcgctgg tggtgctgct cttgcaactg gagctgcgcg
    26941 cccagcgccg catgcgcttt gccaccggcg gcgtgggtcg cgccggctcc agcgaggccc
    27001 agcccacgcg gctgcacggc gcgtatgccg tgctggcctg gctggtgtgc gccctgccgg
    27061 tgctgatggg ttttgcgctg ccggtgctgt tcatgctgcg gccgctgctg gccgactggt
    27121 cgctgctgcc ctgggatcgc ttcgtgcaat gggccggcaa cagcgtgcgt ctgggcgcca
    27181 tcaccgccgt gctggcggtg ggcatcgcgc tggctctggc ctttgccgtg cgccgccggc
    27241 gcgggccgct gatgcgtgcc gtggtgcagc tggtcagcct gggctacgcc gtgccgggcg
    27301 cggtcatcgt cgtcggcctg ctgctgcccg tgggctggct gcaggccgcg gcccccacct
    27361 ggggcctgag cgcgctggtg accaccaccg ccgtgggcat cgtctgggcg tacctggtgc
    27421 gcttttgcgc agtggccctg cagtcggtgc aaagcggcta tgcgcgcgtg ccggccagcc
    27481 tggacgattc ggcgcgcatg ctgggcgtgg gcggcatggg actgctcatg cgcgtgcact
    27541 ggccgctgct caagcgatcg acggccgccg ccacgttgct ggtgttcgtg gacgtgatga
    27601 aggagctgcc cgcaaccatc gtgctgcggc cctttgacag cgataccctg gccgtggtgg
    27661 cctaccagct ggcgcgcgac gaacgcctgg gcgaggccgc gctgccctcg ctggcgctcg
    27721 tcgccgtggg cctggtgccg gtgatcttgc tcagccgcac gctgcgcggg tcttcgcgct
    27781 gatgcctaca ctgcggccat gaacgacatc accatctacc acaatgcccg ctgcagcaac
    27841 tcgcgcggcg cactggaaat cctgcgcgaa cgcggcatcg agcccgtcat cgtggactac
    27901 atcaaggaac cgctggacgc ggcccggctc accgccctgg tggcacgcct gggggtgccc
    27961 gtacgcgagc tgatgcgcag caaggaggcc ctctaccagg agctgggcct ggccgcgcca
    28021 gagcgcagcg acgccgagct gatcgccgcc attgcagagc acccggtgct gctcaatcgc
    28081 cccatcgtcg ttacgcccaa gggcgcgcgc ctgtgccggc cgccggagct ggtgctcgat
    28141 ttgatcgctt aagaatcacc taagccagcg ccaccacact gccttccatc agcactacaa
    28201 gcttttggaa aggcagacca tgaacaccct tctcacctct ccccgccgtt tggtactggc
    28261 cctggtggcc gcgggcgccc tgggcgccac gggtgccggc ctgatcacgg cgcatgaggc
    28321 gcgcgcgcag ctggccatgc ccgtggcggc cgcagtggcg ccggccacgc ctgccgcagc
    28381 cgtggcgctg cccgattttt ccagcatcac cgcgcgctat ggcccggcgg tggtcaacat
    28441 cagcgtcagc ggcatgcgca agaccggggg cgacgatggc gacaacagcc ccgcggcgcg
    28501 ccaacgcgga ccgcagggca tggatccgaa cgatccgttc aacgagttct tccgccgttt
    28561 cggcattccc atgcccgagc gcggtggcca gggccagcgg ggcatgccca tgcgtggcga
    28621 gggttcgggc ttcatcatcg atgccaatgg catcgtcctg accaacgccc atgtggtcaa
    28681 gggcgcgagc gacgtgacgg tgaagctgac cgaccggcgc gagtttcgtg ccaaggtgct
    28741 gggtgccgac cccaagaccg acatcgccgt gctgaagatc gacgccaaga acctgcccac
    28801 ggtgaagctg ggcaattcgc gtgacctgaa ggtgggcgac tgggtgctgg ccataggttc
    28861 gcccttcggc ttcgagaaca cggtcaccgc cggggtggtc agcgccaagg gccgctcgct
    28921 gcccgaggac agctatgtgc ccttcatcca gaccgatgcc gccgtgaacc ccggcaactc
    28981 cggcggcccg ctgttcaatg ccgccggcga ggtggtgggc atcaactcgc agatctacag
    29041 ccgctccggc ggcttccagg gcctgtcgtt tgccatcccc atcgaggtgg cgacgcgcgt
    29101 ggagcagcag atcgtggcca ccggccatgt cgagcatgcg cgcctgggcg tggccgtgca
    29161 ggaggtgaac cagacctttg ccgactcgtt caagctgccc cggcccgagg gcgccctggt
    29221 ggccagcgtc gatgccagcg gcccggctgc caaggcgggc ctgaaggtgg gcgacgtgat
    29281 cctcaaggtc aacggccagc ccatcgtggc ctcgggtgat ctgccggcct atatcggcca
    29341 gcgtgatccg ggcgacaagg tgcagatgga ggtctggcgc cagggtcatg ccgaaaccct
    29401 ggccgccacc ctgggcaatg ccaacgacaa ggccgccaag ctggccagca acgaccaggc
    29461 cgtgggcaag ggccagctgg gtctgacgct gcgcccgctg cagcccgatg aagccaaggc
    29521 cgccggcgtg cccaagggcc agggcctgct gatcgaggat gtgcgcggcc ccgccgccat
    29581 ggccggcgtg cagggcggcg acgtgctgct ggccgtcaac ggcacccccg tcaaggacgt
    29641 ggagcaggtg cgtgccgcga tggctcacgc gggcaagtcg gtggccctgt tgatcgagcg
    29701 cgacggcgac aagatcttcg tgccggtacg cctcggctag gtgcttcacg acttgtagca
    29761 ggcgcagggg tttggcttat gctgccaccc atgcgcctgt tgcttgttga ggacgacacc
    29821 atgatcggcg aggccgtgcg tgacctgctg cgtgccgagc attacgcggt ggactgggcc
    29881 acggatggcg acatggccga cgctgccctg gcggcccagt cctacgacct ggtgctgctg
    29941 gacctgggcc tgccgcgccg tgatggcctg gccgtgctgc gctccctgcg cgcgcgcaag
    30001 gaccgcacgc cggtgctgat cgccacggcg cgcgacggcg tggcccagcg cgtcgaggga
    30061 ctggacgccg gcgccgacga ctatgtgctc aagccctatg acctggacga actgctggcg
    30121 cgcatacgcg cgctcttgcg ccgcgccagc gggcgcgccg agccggtgta cgagcaccag
    30181 ggcgtggcgc tgaaccccgg cacgcgcgag gccacgatcc agggccagcc ggtggtgctg
    30241 tcggcgcgcg aatgggcggt gctcgaaccc ctgctggcgc gccccggcat ggtgctgtcg
    30301 cgccagcagc tcgaggacaa gctctacggc tggggcgacg aggtcagcag caacgcggtc
    30361 gaggtctaca tccacggcct gcgcaagaag ctcggcgccg ccctgattct gaacgtgcgc
    30421 ggcctcggct acatggtgcc caaatgaacc ggctgcgcca ccccgcctcg ctgcgtgtgc
    30481 ggctgctggt gttcctgctg gcggccattg cgctgaccgc cgccgtgcag ggcctgctgg
    30541 cctaccgcag cgcgttggtg gaggcagatg ccctgtttga ttaccagatg cagcagaccg
    30601 cctacgcgct gcgtgctggc ctgcccccgg atgcacgcgc cagcggcaac gccttgccgg
    30661 agtaccagaa cgacgagctc atcgtccagg tctggaccaa cgaggggctg cgcatctttg
    30721 aatccgccgt cggcgcggcc ctgccgcaga aggcggtgct gggctttacc gagctgcagg
    30781 cacgcggcat cagctaccgc gtgttttcgc tgcagacacg ctcgcaggtg atacaggtgg
    30841 cacaggacat gtccgcgcgc cgcgccatgg cgcgtgctct ggcgctgcgc tccttgctgc
    30901 cgctggcggt gatggcgcca ctgctggcgc tggccgtgtg gtgggccgtg agccgcctgc
    30961 tggcccccgt acagcgcgtg cgtggccagc tggcgcagcg ccggccggac gacctgacgc
    31021 ccgtcagcgg cgccggcctg cccagtgagc tgcaacccct ggtgcatgaa ttcaacgcgc
    31081 tgctggcgcg cgtgcagcag gccttcgacg cgcagcagca tttcgtggcc gacgccgccc
    31141 acgaactgcg ctcgcccctg gcggcgctga aactgcagct gcagggtctg cagcgcgcgc
    31201 ccgacacggc cacgcgcgag cgcgccgtgg aacgcctggg cagcggcata gaccgtgcca
    31261 cgcgcctggt cgagcagctg ctggccctgg cgcgccagga ggcgcagata gcggccggcg
    31321 cgccacccca gccggtgccg ctggccgaac tggcgcgcca ggccgtgctc gatgccacac
    31381 ccgccgccca gcagcgcggc atagacctgg gcctggcgcc ggtgccgccc gaggccgagg
    31441 gcgccagcgt gcccggccat gcgcaggccc tggccatcct gctgcgcaac ctgctggaca
    31501 acgccatcaa gtacacgccc cgtggcggcc gggtggacgt ggcgctgcag gccgacgacg
    31561 gccatatcac gctgctggtg gacgacagcg gccccggcat cgcgcccgct gagcgcgcgc
    31621 aggtgctgca gcgctttcac cgcggtgcgc agcccgaggg cgcgccggcg tctggcagcg
    31681 gcctgggcct ggcgattgcc gacagcatcg cccgcctgca tggcgcgcgg ctgcagctgg
    31741 cggatgcgcc gcagctgggc ggcctgcgcg tcagcctgcg cctggcgcgc cacacgccgc
    31801 cacgggagag tccgcacaca tgatcagcgt gaccatgctc tccggcctgg gccacgccgt
    31861 cttcgtggct gcgggcctgt tgatctacat gatgggcacg cgcatcggcc accagcaccg
    31921 ccatccctcg gccgccatgg cctgggtgct gggcctgatc accttcccct acctgacgct
    31981 gccgctgttc ctgttctttg gcacgcgcaa gtttgcccgg ccggcgcgcc gcgagcgcca
    32041 ccaggtgctg gccattcccc agggtggccc gagctgggcc acgcggctgc tggccggcat
    32101 ggagttggcg cccgccgtgc acaacgaacg ggtgcagctg cacgccaatg gcgacgaggc
    32161 gttgcgcagc ctgctggcca tcatggcatc ggcgcgccag cgcctcgatg tctgcaccta
    32221 cctgttcgcc aacgacgaca ccggccgcca gattgcgcag cagctcgcgg ccagcgcggg
    32281 ccgtggcgtc agcgtgcgcc tgctggtgga cgccatgggc agcatgggca aggcccccgc
    32341 catgctgcgc gcgctgcagc ggcagggcgt gcaggtgcgc cgcttcatgc cgctgctgca
    32401 caacccgctg cacggccgca tcaacctgcg caatcaccgc aagctggtgc tggccgacgg
    32461 acagcgcctg tggagtggtg ggcgcaacct ggccagcgag tactttgtcg atcgccccgg
    32521 ccagcccgcc tggatagacc tgagctacga ggtgcagggc ctgctggccg cccaggccca
    32581 ggcccagttc aacaccgact ggcgcgtggc acacggcctg gcgctgcgca aggcgcgccg
    32641 gccgcacggc acgcccggcg tgcaccagca gcccgtgggc ggcccgctgg cccaatggct
    32701 gcccagcggg cccgaccatg ccgacgacac cgtgcatgcg ctgctgctgg ccgcggccta
    32761 ccacgcccag cggcgcatcg tcgccgtcac gccgtacttc gtgcccgcgc ggcttcgaca
    32821 ccggcccggc gaacggcctg atggacgcct ggtgcctggc ctgccggcgc ggcgtgcgcg
    32881 tgagcctgct ggtgccggcg cgctccaacc accgcctggc cgacatcgcg cgcgagcgcg
    32941 ccctgcgcca gctcgctgcc gccggcgccc aggtctggct ggcaccggcc atggtgcatg
    33001 ccaaggccgt gctcatcgac gacgacctgg ccctggcggg ctcgctgaat ctcgacgcgc
    33061 gcagcctgtt tttgaactac gaggccatgg ccgccttcta tggcgagcag gagctgcgct
    33121 ggctctcgca gtggtgcgcc cggctcatcg ccacggctcg cccctaccac gcgcgccagc
    33181 cgtcctgggc gcgcgacatt gttgaagggg tggtgcggtc ggtgggtttt cagctgtagg
    33241 ccgggcctgt cgggccgcca ggggcagtgg gtcggtcatg cgctgcgcgt ctccatcgct
    33301 caaagccagt tttccacttt gaggccgcga atgcgggcga actcccgggt gttgttggtg
    33361 accagcgtca gcctcagggt gcaggcgtgc gcggcaatca gcaggtcgtt gccgccgatg
    33421 ggctgccccg ccgcttccaa ctcggcgcgg atgcggccgt agtcgccatc ggccggcaaa
    33481 tccagcgcca gtacgggaat cgtttccagc agcgcctgca ccttggccag cagacggggc
    33541 gatcccttct tggcgcaacc atagcgcagc tccgcggcca ccacgatgct ggtgcaaatg
    33601 cccttggcgc ccagttgcgc gatgcgctgc gcagccgccc ccgacgggtt gcgtatcagg
    33661 tcgctgatga tgttggtgtc cagcaggtag ccgctcacgg caggtcctcg ggctgaaccg
    33721 gggcatcgtc gatggccggg aactgatcct cgggcgccag cggcggctcc tggcgccact
    33781 cggccagtag ctccaggata ttggtggggc gcaacaccgg ctcgatgatc agcttgctgc
    33841 cttcgcggtg aatcagcacg cgatcgcccg gcaattcgaa ctcgaccgga atgcgcaccg
    33901 cctggctgcg gttgttgcga aacagcttgg cctctttggg ccgggaagac agggttggag
    33961 cagtcatgac acctccgttg gcatatgcaa aattgtatat gccaacgggg ctcgatcaac
    34021 cgccttgggt cagcccgccg gcagcttgac caccttcagc gactctatcc cccggtcgtc
    34081 cacgatcttc accgccacct gctggttcgc tccggcggca aagggcagcg acagcctgcc
    34141 ggtgaattgc tgcaaaaacc cctcatccag cttggcgcgc atgtcttttt tgacttcacc
    34201 cagccctcgt ccttgcccgc catcaggaag aacacctggc gcgggaacag gccgcgcccg
    34261 tcgtaatcgg tgtcgagctg ccaggccgca atcttgcgct tgccgcgaga ggccggttcg
    34321 cccctggcgg tgtcgaagca gtcaaagccg ttcacctgcc acagcccacc cgcagccctg
    34381 atgccacaat cgcctggccc ggcatgtcag gttcttga
//
LOCUS       DAOMTQ010000008        32245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_100_length_32245_cov_25.697856, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000008 DAOMTQ010000000
VERSION     DAOMTQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 32245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32245
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100_length_32245_cov_25.697856"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    216..304
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(354..1601)
                     /locus_tag="PK518_01800"
     CDS             complement(354..1601)
                     /locus_tag="PK518_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM47673.1"
                     /translation="MRIAIVGAGIVGAATAYELAADGHEVTVFEQRGAAAEEASFASA
                     GLLAPALLTPWAQPAMGAGTLRQLWDSEATLRLVRGAGPSGLAWLWRWAQAGRSPTAG
                     GLLSALQQLGQYSLARTRSLAEQLEVDIETSRGALVLLRRPECLQRLQPALQLLREAG
                     STITDIDADTARLIEPGLSADTPLAGALHAPDGEAGNCRLFALVLRQAAQNLGAQFMF
                     GSAVRALTHAPTGLQITGESAPRRFDAVVLCAGLASAALLRPLGPRLPLRALYGYTIS
                     APLREDTHAPQGAVIDPLHRLIITRQGQRVRVSGGAEIGHGGGAHHAPTLQTLYLALS
                     GWFPGGALTSSPQVQVWRGARPMLPDGAPVLGASGVAGLWLNTGHGACGWAQACGSAR
                     ALADTIAGRSADISLEGLDAQRF"
     gene            complement(1650..2996)
                     /locus_tag="PK518_01805"
     CDS             complement(1650..2996)
                     /locus_tag="PK518_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRM47674.1"
                     /translation="MLTDLVSMRSQLAAGQSSAAAELERCIAAAASPACAHVFRQTCL
                     DEARAAAAAPGVQLLPLAGLAVSVKDLFDIKGLPTAAGSSVLAGAAPATADCSAVARL
                     RRAGGAILGHSQMVEFAFSGVGVNPHFGTPAAHDARFGALTGPARVPGGSSSGAGVSV
                     AAGATFVGLGSDTGGSIRIPAALNGVVGFKNTARLVPTDGAVPLSTTLDTACAITRSV
                     RDAIVVHEILAARRVTRSPAPLPAWRLAVPSTLFLDSLDATVAAAFERSLDQLRRAGA
                     QITEIALPQTGELAEVNATGGFSAAESYAWHRPLLQQHAASYDPRVRARIERGATMSA
                     CDYINLQHARRAWIARMEAAVAGFDALLSPTVPIVAPTIASVAPGSERDAEFFRVNAL
                     LLRNTSVVNMLDGCALSLPCHQAGELPVGLMVWHAALRDDAVLNIGLLIEQELQKQ"
     gene            3187..3939
                     /gene="rpsB"
                     /locus_tag="PK518_01810"
     CDS             3187..3939
                     /gene="rpsB"
                     /locus_tag="PK518_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRM47675.1"
                     /translation="MSVTMREMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIINLEK
                     SLPMFQEAQKFAKQLAANRGTILMVGTKRQARELVAEQAQRAGVPYVDQRWLGGMLTN
                     FKTVKTSIKRLKDMKAQQEAGLESMSKKEQLMFARELEKLEKDIGGIQDMNALPDAIF
                     VIDVGFHKIAISEAKKLGIPLIGVVDSNHNPEGIDYVIPGNDDSAKAVALYARGIADA
                     ILEGRANAVDEVVKAVAAEGSDEFVEVQDSAA"
     gene            4114..5007
                     /gene="tsf"
                     /locus_tag="PK518_01815"
     CDS             4114..5007
                     /gene="tsf"
                     /locus_tag="PK518_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRM47676.1"
                     /translation="MAAITASMVAELRAKTDAPMMECKKALTEADGDMAKAEELLRVK
                     LGTKAGKAASRVTAEGVVAASISGNLGALIEVNSETDFVSKNDSFIAMANAAAKLVAE
                     HNPANIEALGQLAYEQDGFGPTLEDVRKGLIGKIGENMSFRRFKRFAGNNLAHYLHGS
                     RIGVVVEFDGDAVAAKDVAMHVAAMKPVAVTSAEVPADLIEKERSVAAAKAAESGKPA
                     DIAAKMVEGSVQKYLKEVSLADQVFVKAADGKQTVAAMLKGANTTVKGFTMYVVGEGI
                     EKKTDDFAAEVAAQVAAAKGA"
     gene            5059..5781
                     /gene="pyrH"
                     /locus_tag="PK518_01820"
     CDS             5059..5781
                     /gene="pyrH"
                     /locus_tag="PK518_01820"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRM47677.1"
                     /translation="MTNAAPAHKRILLKLSGEALMGDDAFGINRNTIVRMVQEVVDVT
                     RLGVQVAVVIGGGNIFRGVAGGAEGMDRATADYMGMLATVMNSLALADAMNKQGVTAR
                     VMSAIDIQQVVEPYVRPKALQYLEEGKVVVFAAGTGNPFFTTDTAAALRGAEIGAELV
                     LKATKVDGVYTADPMKDPTATRYAKLTFDEAMVRNLGIMDATAFALCRDQKLPIRVFS
                     ILKPGALKRVVMGEDEGTLVYA"
     gene            5821..6381
                     /gene="frr"
                     /locus_tag="PK518_01825"
     CDS             5821..6381
                     /gene="frr"
                     /locus_tag="PK518_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRM47678.1"
                     /translation="MTIADIKKTLDTKMDQSITALKNNLSKVRTGRANPQLLDSIHVE
                     YYGSMVPLSQVANVALLDARTISVQPWEKNMGAKIEKAIRESDLGLNPASLGDLIRVP
                     MPPMSEERRKEMTKLARNEGENSKVAVRNLRRDANEAVKKLVKDKLASEDEQKRAEAD
                     IQKTTDKHIAEIDSLVAAKEQDILAI"
     gene            6428..7183
                     /gene="uppS"
                     /locus_tag="PK518_01830"
     CDS             6428..7183
                     /gene="uppS"
                     /locus_tag="PK518_01830"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRM47679.1"
                     /translation="MTHNAPRTTPDGGHGPQHIAIVMDGNGRWATKRFLPRLAGHRQG
                     VESLRRCVRACVARGVRVLTVFAFSSENWNRPADEVSGLMELMGKALVREVPQLTKDG
                     VRLRFVGERAGLSAGMRAALEQAEAATAANVRMTLNVCFNYGGRWDIAQAAATLAARG
                     EPITEASLHGAMGMSHVPDPDLLIRTGGEKRISNFLLWQAAYSELYFSERLWPDFDEA
                     ALDAAIADYAARERRFGLTSEQLQPAQPDPLRA"
     gene            7226..8074
                     /locus_tag="PK518_01835"
     CDS             7226..8074
                     /locus_tag="PK518_01835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRM47680.1"
                     /translation="MLKQRVITALVLLAILLPALFYPSFVPFAVVTLVMMAAGAWEWG
                     RLNGLGHGASLVLGALCLALCSLSWAAGWLQLALPWLWTLGGAAWVLGGAWLLRVGVD
                     GWPRVPRALRIVGGVLALWLAWLAVAQARVAGINFLLSVLTLVWAADIFAYFAGRAFG
                     LRFTKGKLAPSISPGKSWEGVWGGMAGVLALALAWVWADAHWQTAVPSLYTHLARRGW
                     WLLLIGALFMAAMSVVGDLVESLIKRSAGVKDSSGLLPGHGGVLDRIDALLPTLPLAM
                     MLSHYS"
     gene            8078..9253
                     /gene="ispC"
                     /locus_tag="PK518_01840"
     CDS             8078..9253
                     /gene="ispC"
                     /locus_tag="PK518_01840"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRM47681.1"
                     /translation="MKQRLTVLGSTGSIGTNTLDVVARHPDRYEVFALSAATQVDLML
                     AQCAQFCPRYAVMASAPHARLLAERLKQNDLSTQVLQAPDALETIASHDQVDAVMAAI
                     VGAAGLAPCLAAARAGKRLLLANKEALVVGGAVFMAAVRQGGATLLPIDSEHSAIFQG
                     LPEDASTWARRVDHILLTASGGPFRTRDPATLRAVTIEEACAHPNFSMGRKITIDSAT
                     MMNKALEVIEARWLFDLAPEHIKVVIHPQQIIHSMVQFKDASIIAQLGTPDMRVPIAC
                     GLAWPERIASGAPLLDFTQLAALTFEEADARRFPGLHLSWQALAAAPGTTAVLNAANE
                     VAVEAFLQQRIRFDQIHALNVATLAAVQPSNPDTLHALLALDAETRAVAQELAQRLN"
     gene            9357..10718
                     /gene="rseP"
                     /locus_tag="PK518_01845"
     CDS             9357..10718
                     /gene="rseP"
                     /locus_tag="PK518_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRM47682.1"
                     /translation="MLTIAAFIVALGVLIAVHEYGHYRVAVACGVKVLRFSVGFGRPL
                     LRWQRKGSPTEFVIGAFPLGGYVRMLDEREAPVAAEDRHLAFNTQPLRARAAIVAAGP
                     LANLLLAVLLYATVNWSGVDEPKAVLASPVAGSIAQQAGLHGGELVLGAALGEGDFEP
                     VRSFEDLRWTLTRGALDGESVRLQFQPQRGSAQRELVLPLDKLEVREADAHLFRRIGI
                     VGPWTRPVLGELVAGGAAERAGLRAGDLVLRVGSGDVVDGSQLRELIRTSGRGGQPQA
                     LAWRVERDGRQLDLLVQADVVQEASGSVGRIGAYVGAAPEMVNVRYGATEGLWKGAER
                     TWEVSVLTLRMLGRMVIGEASVKNLSGPLTIADYAGRSASMGLTQYLAFLALISVSLG
                     VLNLLPLPVLDGGHLMYYLWEALTGRSVSDAWMERLQRGGVAVLLLMMSIALFNDLTR
                     LFG"
     gene            10763..13060
                     /gene="bamA"
                     /locus_tag="PK518_01850"
     CDS             10763..13060
                     /gene="bamA"
                     /locus_tag="PK518_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRM47683.1"
                     /translation="MRKHINRLGVRTASALVAMAFATQAAWALAPFKVQDIRVEGLQR
                     VEPGTIFASLPLRVGDEYNDEKGAAAIRALFALGLFKDVRLEATGNVLVVVVEERPTI
                     ADVEFAGTKEFDKDTLKKAMRDVGLTEGRPFDKALADRAEQELKRQYINKSLYGAEVV
                     TTVTPIERNRVNLTFTVTEGEPAKIKDIRIVGNQAFSESTLKGLFDQDTGGWMSWYTK
                     SNRYARAKLNADLETLRSYYLARGYLEFRIDSTQVAISPDKQDISITINVTEGQRYVV
                     SGVKLEGSYLDRDDEFKSLITIRPGEPYNADKVAETTKAFTDHFGNFGFAFARVEAVP
                     EIDRENNRVALVLRAEPARRAYVRRIQVSGNNRTRDAVVRREFRQYEASWYDGDKIKL
                     SRDRVDRLGFFTEVNVETQEVPGSPDQVDLVINVVEKPTGSLQLGAGFSSAEKVSLSF
                     GIKQENVFGSGNYLGVDVNTSKYRRTLVFSTTDPYFTQDGISRTLDLYYRTAKPYQHQ
                     GGDYELVTAGASVRFGVPFSENDTVFFGGGLEQTQIKSGTNIPAAYLAYAREFGDTSW
                     SLPLTIGWSRDNRDSALAPNSGRYQRLNTEWAVAGDARYIRGNYQYQQYIPLNKQYTF
                     AFNGELGLGKGMNGQPFPVFKNFYSGGLGSVRGFDQGTLGPRDVTGASLGGPKKVTLN
                     AELIAPFPGAGNDRTLRWFAFVDAGNVYGENDNWELSELRASAGLGLSWISPLGPLRL
                     AYAQPVRKFAGDRIQKLQFQIGTSF"
     gene            13060..13584
                     /locus_tag="PK518_01855"
     CDS             13060..13584
                     /locus_tag="PK518_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRM47684.1"
                     /translation="MKSLSRHIPLVFLLGSLAAAVPAQAQEFKAGFVNTDRIFREANT
                     AKTAQAKLEQEFSKREKELVDMGNALKSATEKFEREAPTMAESQRVTRQRQLVDQDRD
                     FQRKRREFQEDLGARKNEELGQVLERANKVVKQLAEAEKYDVILQEAVYINPKHDITD
                     KVIKAMNAAGNSGK"
     gene            13613..14611
                     /gene="lpxD"
                     /locus_tag="PK518_01860"
     CDS             13613..14611
                     /gene="lpxD"
                     /locus_tag="PK518_01860"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRM47685.1"
                     /translation="MSLRLGQIVDALGGSLEGASADTQIQRIAPLETAGEGDLAFLNN
                     PRYQSQLAASRAACVIVAPAMRAAALARGACIVTDDPYAYFARVTQLWVRAQGQAPVP
                     GIHASAVVDAQAQVHPTATIGPLCVVERGAVIGAHTVLKSRVTVAERCTVGARCILHP
                     GVVIGADGFGFARQQGEWIKIEQLGAVRIGDDVEIGANTCIDRGALEDTVIEDGVKLD
                     NLIQIAHNVHIGRHTAMAGCSAVAGSTRIGAHCTIAGAASIVGHLQLADNVHVSTNTV
                     VTHSIARPGQYTGVFPMDENSKWEKNAATLRQLYKLRERIKALEQARQGDQSATQQ"
     gene            14608..15051
                     /gene="fabZ"
                     /locus_tag="PK518_01865"
     CDS             14608..15051
                     /gene="fabZ"
                     /locus_tag="PK518_01865"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRM47686.1"
                     /translation="MMDIHAILKQLPHRYPFLLVDRVVELERNTRIRAIKNVTFNEPY
                     FTGHFPGRPVMPGVLILEALAQAAGLLAFDAMGQVPDENNIYYFVGIDGARFKRPVEP
                     GDQLILEITIDRVRGGVWKFNGVARVGDEVACEAQLMCTMRNVGG"
     gene            15063..15851
                     /gene="lpxA"
                     /locus_tag="PK518_01870"
     CDS             15063..15851
                     /gene="lpxA"
                     /locus_tag="PK518_01870"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRM47687.1"
                     /translation="MSQIHATAIVAAGAQIDPSATVGPYAIIGANVRIGARTSVGPHC
                     VIEGHTTIGADNHIFQFASLGAAPQDKKYAGEPTRLEIGDRNTIREFCTFNTGTVQDQ
                     GVTRIGDDNWIMAYVHIAHDCVVGNQVILANNATLAGHVQVGDQVIIGGLTGVHQYSR
                     IGAHAMAGFASHISQDVPPFMMVDGNPLAVRGLNLEGLRRRGFSAQRVAAIKQAYRLL
                     YRQGLTLEAALSAMAELPQQHPEADGDIALLRDFIAASQRGIAR"
     gene            15868..17016
                     /gene="lpxB"
                     /locus_tag="PK518_01875"
     CDS             15868..17016
                     /gene="lpxB"
                     /locus_tag="PK518_01875"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRM47688.1"
                     /translation="MAGISPRVAMVAGETSGDLLAGLLLDGLRAQWPGVASMGIGGPR
                     MAERGFDAWWPSERLAVHGYSIELVRRLLGILKIRKQLRTRLLADRPDVFIGVDAPDF
                     NLGLEADLRNSGVKTVHFVCPSIWAWRANRVDKIRAAADHVLCIFPFEPELLARHGIA
                     ATYVGHPLASVIPMQPDKLAARAQLGLAPQDEVLAILPGSRSAEVAYIAKPFFQAAAL
                     ISKARNAIKIVVPAVPALRARIEQIARDCGVLEQLQIVTGQSHQVLAACDVTLIASGT
                     ATLEAALFKRPMVIAYHMHPISWRLMRGKQLQPWVGLPNILCRDFVVPELLQDAATPE
                     ALSGAVQQWLDAPPARLDALHQRFTALHEQLQRDTPRLAANAIQEILA"
     gene            16991..17626
                     /gene="rnhB"
                     /locus_tag="PK518_01880"
     CDS             16991..17626
                     /gene="rnhB"
                     /locus_tag="PK518_01880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRM47689.1"
                     /translation="MPSKKSWLEQGNLNWHAPGLLVAGVDEAGRGPLAGPVVAAAVIL
                     DELQPIAGLADSKALTAARRERLFDEIRAKALCCSIAEASVEEIDRLNILQATMLAMR
                     RAVQGLRLKPRLALVDGNRLPVLDVPAEAVVKGDAKVAAISAASIVAKVTRDRWCAQL
                     HDQYPQYGFAGHKGYGTAEHLAALQAHGACPQHRRSFSPVARALQAAPAQP"
     gene            17623..18399
                     /locus_tag="PK518_01885"
     CDS             17623..18399
                     /locus_tag="PK518_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRM47690.1"
                     /translation="MTRQQPTLIQSRDNPLLKDLRRLALDSTAYRKQGRVWLEGDHLC
                     GAALVRGQRPAIAVFSESFWPLAPAEYAQAAIKTIVMPDALWRDISGLESPARMGFVL
                     PLPVEQGLDAQAPTVVLDRLQDAGNVGSILRSASAFGFTQIAALKGTAALWSAKVLRA
                     GMGAHFALRLLEGLELQDIDRLAVPLLATSSHQGDWLHRAALPWPCAWIMGHEGQGVS
                     PELQLRARQHIRITQPGGEESLNVGAAAAICLHASAAARG"
     gene            18425..18808
                     /locus_tag="PK518_01890"
     CDS             18425..18808
                     /locus_tag="PK518_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM47691.1"
                     /translation="MIDALISGKLHAHPEQRTSKTGKTYATAKMTAAGGDGQSLFVNC
                     IAFDEASVEALLALVAGDSLAVAGSITPKVWTDREGQHRPALDMVVARVLTAYHVTHK
                     RRAMQQSAAPQARQQPMDDGEPLDF"
     gene            complement(18863..18938)
                     /locus_tag="PK518_01895"
     tRNA            complement(18863..18938)
                     /locus_tag="PK518_01895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18903..18905),aa:Thr,seq:cgt)
     gene            complement(18951..19958)
                     /locus_tag="PK518_01900"
     CDS             complement(18951..19958)
                     /locus_tag="PK518_01900"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRM47692.1"
                     /translation="MTERISLTRYLVEQQRVDGRIPPQLRLLLEVVARACKRISQAVN
                     KGELGDVMGTAGSQNVQGEVQKKLDIIANETLIEANEWGGHLAAMASEEMEGIYVVPN
                     RYPQGEYLLMFDPLDGSSNIDVNVSIGTIFSVLKKPEGHPGVHDNDFLQPGTCQVAAG
                     YCIYGPQTTLVLTVGDGVAMFTLDREQGSFVLIRENVRIPEDTKEFAINMSNMRHWDE
                     PVKRYIDECLAGKEGPREKDFNMRWVASMVADVHRILSRGGIFMYPWDKREPNKPGKL
                     RLMYEANPMGWLVEQAGGAATNGKQRILDIKPTQLHERVSVILGSKNEVERVTSYHNG
                     I"
     gene            complement(19982..22690)
                     /gene="pepN"
                     /locus_tag="PK518_01905"
     CDS             complement(19982..22690)
                     /gene="pepN"
                     /locus_tag="PK518_01905"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRM47693.1"
                     /translation="MKEGQAKAIERLDYAPPAFWIDSVDLTFDLDPAKTRVLNKMQVR
                     RNSAAAPQALRLLGQELNLARVLVNGAGTSFKIEGDELVLENLPEGDAPFALEIFTTC
                     APAKNTQLSGLYVSQGTFFTQCEAEGFRRITYFLDRPDVMASYQVLLRASKADYPVLL
                     SNGNLVQSGELEDGRHFARWQDPHKKPSYLFALVAGKLVAREQQVTSRAGNKHLLQVW
                     VRPGDLGKTEHAMNSLMASIAWDEARFGLTLDLERFMIVATSDFNMGAMENKGLNIFN
                     TKYVLASEATATDTDFANIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEF
                     SQDMAGSPSARAVKRIEDVRVLRTAQFPEDAGPMAHPVRPDSYVEINNFYTVTIYEKG
                     AEVVRMQHRLVGREGFARGMKLYFERHDGQAVTCDDFAQAIADANPASPLALHLEQFK
                     RWYSQAGTPHVKAVGQYDADAGRYTLTLSQSCAATPGQPNKLPFVIPVALGLLDAQGN
                     ALPLQLDGEATAGSTERTVVLTEESLTLGFVNVTSQPVPSLLRGFSAPVVLDYDYSDT
                     ELLTLLAHDGDAFNRWEAGQRLMLRIAINEIADSTITEGANGQIDQNLLPDALVQAMR
                     AVLRHPQLDAAFKELVLTLPSESYIAEQLTQVDPQRVHAVREALRLQLALSLQADWAW
                     AWDEHQASGAYRPDATSSGRRALAGLALAMLCLAARATGDEVWPGKTYQRFKIAANMT
                     DRFNALSALVMSGHELAQPALARFHALFKDDALVLDKWFALQAGSTDRGGNVLPAVKA
                     LLKHPDFHIKNPNRARSVIFSYCSANPGAFHRRDAAGYVFWAERVLELDALNPQVAAR
                     LARALDRWSKLAEPYRTAAREAIARVAAKADLSNDVREVVTRALAD"
     gene            complement(22709..23813)
                     /gene="prfB"
                     /locus_tag="PK518_01910"
     CDS             complement(join(22709..23731,23733..23813))
                     /gene="prfB"
                     /locus_tag="PK518_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518358.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRM47694.1"
                     /translation="MEAEHINQIGNTLEDLSARTAELRRYLDYDAKYERLRTVNASLE
                     DPACWNDPKKAQELGKEKKSLDAVVLTLQKLTAELADNRELYEMSYEEDDEAGLLTIE
                     AETAKLLPLIEELEFRRMFGREADPLNCYIDIQAGAGGTEACDWASMLLRQYLKYAER
                     KGFKATVDEETPGDVAGIKSATIHIEGDYAYGLLRTETGVHRLVRKSPFDSSGGRHTS
                     FASLFVYPEIDDSIEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHMPTGIVVQC
                     QDGRSQHSNRDVAWQRLRSKLYELEMQKRMEAQQALEETKTDVGWGHQIRSYVLDNSR
                     IKDLRTNVEISATQKVLDGDLDAFIEASLKQGL"
     gene            complement(24002..24997)
                     /locus_tag="PK518_01915"
     CDS             complement(24002..24997)
                     /locus_tag="PK518_01915"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HRM47695.1"
                     /translation="MNDKQPAIRIAIVGHGNLGRGVESAIANNPDMTIAGIYTRRDPA
                     QLQPLTAGVSVYPMDALAGHKDRIDVLILCGGSKDDLPRQSPELAAQFNLVDSFDTHA
                     RIPAHFAAVDAAAQAGKTTALISAGWDPGMFSINRVLGEALLPDGATYTFWGKGLSQG
                     HSDAIRRVSGVAGGVQYTIPVPEAVDQVRSGVRPQLTTREKHRRDCFVVLAPGADAEA
                     VRQAIVTMPHYFDEYDTTVQFISAEQLAREHGAMPHGGFVIRSGNTSAANKQVIEYRL
                     QLDSNPEFTSSVLVAYARAVHRMAQAGQFGCKTVFDVAPGLLSPQSPAELRERLL"
     gene            complement(25115..26047)
                     /locus_tag="PK518_01920"
     CDS             complement(25115..26047)
                     /locus_tag="PK518_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM47696.1"
                     /translation="MYQALRPSRSDSVRIRTLNYHVRTWGAPQPGTAPLLLLHGWMDV
                     GASWQFVVDAFGEAFAAGRLIIAPDWRGFGGSRLAAPCDGHHFVDYLADLDQLLDHYA
                     GAQPVDLVGHSMGGNVAMMYAGARPARIRRLVNLEGFGMAPSNPAQAPGRYAQWMDEI
                     RQWERGEMDLKPYDSADGVALRLMKTNPRLASDKACWLARHWAGADAQGRWHILGDAA
                     HKIVNPHLYRVDEALALYAAITAPVLSVHASDDSLGRWWKGRYTLQDYHQRLAHVADS
                     RSALVQDAGHMLHHDQPQAVAALIEDFLADKMPA"
     gene            26174..27499
                     /locus_tag="PK518_01925"
     CDS             26174..27499
                     /locus_tag="PK518_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM47697.1"
                     /translation="MQRSIVAAALAAGFCGLALPAIAGQVTVITSFPKELTTAYQKAF
                     EAAHPGIKIEILNKNTTAAVAYIRELPEGQRPDVMWASAPDAFEVLASYQLLQKAPEV
                     VNKSAPEKIGNYPLNDPKGMYYGQALAGYGISWNTRYIKAHKLAAPREWSDLTRPEYF
                     GHVAISSPSRSGTTQLTVETILQGEGWDKGWSQLLQIMGNAAAVTDRSFAVPDGVNNG
                     QYGIGIVIDFLALAGKYSGYPVDFTYPSMTAVVPANIALVAGAKNAAEAKQFMAYTMS
                     AAGQQILFDPKIGRLPILPYSMLKAPAGYPVPQDIAKRAKVQFNTELSGQRYPVVISL
                     FDQMVTFRLKELQAATKSIHEATAALKARPNARGSELLAQARSLAYTPLVGADNVKNP
                     EFLELFRKSRRDVAVAKELTGMEQLWSEKARANYVRAKQLADEARGLAR"
     gene            27558..29282
                     /locus_tag="PK518_01930"
     CDS             27558..29282
                     /locus_tag="PK518_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM47698.1"
                     /translation="MPVAHLSTAARAPRASAGLGPWIAFALILAFLLVFLILPVGTVI
                     YTAFVTDTGALTVGHFGNFFDQLVFRESFFNSLWVALASTFFASVIALPLAYLTVRFE
                     FRGALLIQTLGVLPLIMPPFVGAVALQLIFGRSGSVNLLLNDWFDITIPIMDGLVGVT
                     FVESIHYFPFILLNLVAAMRNIDGAMEESALNLGARGWRLFRRVIFPLAMPGYLAGAA
                     LVFVKVFDDLGTPLVMGVTNMLAPQAYLRITSVGVDDPLGYVISVIMIVFSILALWLA
                     ARVMKGKDYATLQKGGGALQKRRLSKWEALLAYGWIALVLAVVLAPHVGILLMSLAQV
                     WSFSVLPDSYTLAHYATVFSDARGMMANTLLYCVLAAGLDVVIGTAIAYLILRTRLPA
                     RQWLDWLASAALAIPGLVLAIGYLRLFKGVTLPGSDQLVVQTWVLIMLAYAVRRLPYA
                     LRSCMAALQQVHVSLEEAAQSVGASRLSTIRRVTVPLMAGGILAGFVTSFITAAVELS
                     ATLVLVSTESQAPMSLGIYLYMQSIAGRGPGAALGVLAVLVVGLGTYFSHRFVERSRA
                     TVASSAKA"
     gene            29285..30370
                     /locus_tag="PK518_01935"
     CDS             29285..30370
                     /locus_tag="PK518_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47699.1"
                     /translation="MNQASLECRHISLSYGATPVLRDVNLKIEPGEFFALLGPSGSGK
                     STLLRLIAGFNQHQSGELLIDGQDISGVPPHLRGVGMVFQNYALWPHMTVWDNVAFGL
                     VERRESRDAIRRKVGAALELVGLQDYGQRRPGQLSGGQQQRVALARTVVIEPKLLLLD
                     EPLSNLDKQLRVQMREELKNLQRQLGLTTIFVTHDQEEAMTTADRMAVLDQGVLQQVG
                     TAAGLYDEPHNRFVAGFVGTANLLEGQVSALDGDTLVFDAQGLGRLTLPLPAEPPAPG
                     AAALAFRPHQIRIAAEGEPGDASRVWLQGRVEAAEFLGEFARYRVRVGEVALVADQPH
                     YAGGAMFPPGAQVRLGLAPTQLRYLHR"
     gene            30437..31498
                     /locus_tag="PK518_01940"
     CDS             30437..31498
                     /locus_tag="PK518_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM47700.1"
                     /translation="MNTIPLGQSDLQVTPICLGTMTFGEQVDEPLAHAILDRALERGL
                     NFIDTAEMYAVPARAATYGATEAIIGRWLACRPGARAKVVLASKVAGPSRGMPWIREG
                     SGMTAADIRASCEASLRRLQTDCIDLYQIHWPERHVPVFGQLYYEPAQETSQTSIREQ
                     LQALAALVQAGKVRHIGLSNETPYGVHEFVRLAEQHGLPRVVSVQNPYGLVNRSWDNA
                     MDESCHRLGVSLLAYSPLGFGLLTGKYDRHAPHEPGAPQQARIARYESVRKQRWGRPE
                     ALAAARRYNQLARDHGLTPVQLALAFCYQSWRVASTIIGVTTLAQLDENLDAWGTVLS
                     PELLAAVDALRWQLRDPGQ"
     gene            31561..32061
                     /gene="ybaK"
                     /locus_tag="PK518_01945"
     CDS             31561..32061
                     /gene="ybaK"
                     /locus_tag="PK518_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRM47701.1"
                     /translation="MAKKDKNAHVSETPATQLLRAQGVEFTEHPYDYVEHGGSAESAR
                     QLGLDEHMVVKTLVMQDQDARPLIVLMHGDRKVSTKNLARQIGAKHVQPCKPEVANRH
                     SGYLVGGTSPFGTRKEMPVFIEESILQLPRIAINGGRRGYLLQLDPQVCVRLLQAKTV
                     QCALAE"
     gene            32092..>32245
                     /locus_tag="PK518_01950"
     CDS             32092..>32245
                     /locus_tag="PK518_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRM47702.1"
                     /translation="MSILLSLAATLAAYLLGSLSFAVIVSRAMGLSDPRSYGSKNPGA
                     TNVLRSG"
BASE COUNT         5202 a        10542 c        10904 g         5508 t
ORIGIN      
        1 cttggggcgt ttgcgtggac gggtcatcgt gctcgccggc gaagacgacg atggattgcg
       61 gggtaaaaag cttggttaga tagtgttggt ccatggtgtt tccaaggtgg ttcgatacgg
      121 gctgtgtgca gtgtgtgtgc cgctaaaaaa cgccattgca tcaacgccat cgcagaggcc
      181 tttgagaggc acgaaacgac aagccgaggc acattnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnacttgc attgactttt tgtgtggcag cagcgggttg tctgggcgcg gtcttaaaag
      361 cgctgtgcgt ccaagccttc caggctgata tcggccgatc gcccagcgat ggtgtcggcc
      421 aaggcgcgtg cgctgccgca ggcctgggcc cagccgcagg cgccgtggcc ggtgttcagc
      481 cacaggccgg ccacgccgct ggcgcccagc accggcgcgc cgtcgggcag catggggcgg
      541 gcgccgcgcc agacctgcac ctggggcgag gaggtcagcg ccccgcccgg gaaccagccc
      601 gacagcgcca ggtacagggt ctgcagcgtg ggcgcgtggt gggcgccacc gccatggccg
      661 atctcggcgc cgcccgagac acgcacgcgc tggccctggc gcgtgatgat caggcggtgc
      721 agcgggtcga tcacggcgcc ctgcggcgcg tgcgtgtcct cgcgcagcgg ggcgctgatg
      781 gtgtagccgt acagcgcccg cagcggcagg cgcggcccca gcgggcgcag cagggcggcg
      841 ctggccaggc ccgcgcacag caccacggcg tcgaagcgcc ggggggcgct ctcgccggtg
      901 atctgcaggc ccgtgggggc gtgggtgagg gcgcgcacgg cgctgccgaa catgaattgc
      961 gcccccaggt tctgggccgc ctggcgcaac accagggcaa acagccggca gttgccggcc
     1021 tcgccatcgg gcgcatgcag ggcaccggcc agcggggtgt cggcggacag gccgggctcg
     1081 atgaggcggg cggtgtcggc atcgatgtcc gtgatggtgc tgcccgcctc gcgcagcagc
     1141 tgcagcgccg gctgcaggcg ctgcaggcat tcgggccggc gcagcagcac cagggcgccg
     1201 cggctggttt ctatgtccac ctccagttgc tcggccaggc tgcgggtgcg cgccaggctg
     1261 tactggccca gctgctgcag ggccgacagc aggccaccgg ccgtggggct gcggccagcc
     1321 tgggcccagc gccacagcca ggccaggccg gaggggccgg cgccgcgcac caggcgcagc
     1381 gtggcctcgc tgtcccagag ctggcgcagg gtgccagcgc ccatggccgg ttgcgcccag
     1441 ggcgtgagca gcgccggcgc cagcagcccg gcgctggcaa agctggcctc ctcggcggcc
     1501 gcgccgcgct gctcgaaaac ggtgacctca tggccgtcgg cggccagttc ataggctgtg
     1561 gctgcgccga cgatgccggc gcccacgatg gcaattcgca tgaatatggt taaaagtgcc
     1621 tgtagcgctt gtatggtaag cgccaatagc tattgttttt gtagttcctg ctcgatcaac
     1681 aggccgatgt tcagcaccgc gtcgtcgcgc agcgccgcgt gccagaccat caggcccacg
     1741 ggcagctcgc ccgcctggtg gcagggcagc gacagggcgc agccgtccag catgttgacc
     1801 acgctggtgt tgcgcagcag cagggcgttg acgcggaaga actcggcgtc gcgctcgctg
     1861 cccggcgcga ccgaggctat ggtgggcgcg acgatgggca cggtgggcga gagcagggcg
     1921 tcgaagcccg ccacggccgc ttccatgcgc gctatccagg cgcggcgtgc atgctgcagg
     1981 ttgatgtagt cgcaggcgct catcgtggcg ccgcgctcga tgcgcgcccg cacgcgcggg
     2041 tcatagctgg cggcatgctg ctgcagcagc gggcggtgcc aggcgtagct ttcggcggcc
     2101 gagaagccgc cggtggcgtt gacctcggcc agctcgccag tttgcggcag ggcgatctcg
     2161 gtgatctgcg cgccggcacg gcgcagctgg tctaggctgc gctcgaaggc ggccgccacg
     2221 gtggcatcca ggctgtccag aaacagggtg gaaggcacgg ccaggcgcca ggccggcagc
     2281 ggcgccgggc tgcgcgtgac gcggcgcgcg gccagtattt catgcactac gatggcatcg
     2341 cgcacgctgc gcgtgatggc gcaggcggtg tccagcgtgg tcgatagcgg cacggcgcca
     2401 tcggtgggca ccaggcgcgc cgtgttcttg aagcccacca cgccattgag cgccgccggg
     2461 atgcggatcg agccgcccgt gtccgagccc aggccgacaa aggtcgcgcc cgccgccacc
     2521 gacacccccg cgcccgagga cgaaccgccc ggcacgcgcg ccgggccggt caatgcgcca
     2581 aagcgcgcgt catgggcggc cggcgtgccg aaatgcgggt tcacgcccac gccggagaag
     2641 gcaaattcca ccatctgcga gtggcccagg atggcgccgc cggcgcggcg cagccgtgcc
     2701 acggcgctgc agtcggccgt ggccggcgcg gcgccggcca gcacgctgga gccggcggcc
     2761 gtgggcaacc cctttatgtc gaacagatcc ttgaccgaca ccgccaggcc ggccagcggc
     2821 agcagctgca cgcccggcgc ggcggccgcg gcgcgcgcct catccaggca ggtctggcga
     2881 aatacatggg cgcaggcggg gctggccgcg gcggcaatgc agcgctccag ctctgcggcg
     2941 gcgctggact gcccggcagc cagctggctg cgcatggaga caaggtcggt caacatgagg
     3001 ctatgctaga atccttgggt tttgctgagt gctgcccggc tttgatcggt gccagtcatg
     3061 cggcaaaacc aatcgcaaac cagccctttc aaggtgttgt ggcctgcaaa agtacatgta
     3121 tgaaacacat gttgcacggc gcaattattg gggctggatt ttagacccaa ccttcggagt
     3181 actttcatgt ccgtcaccat gcgcgaaatg ctggaagccg gtgtccattt tggccaccag
     3241 acccgcttct ggaaccccaa gatggccccc ttcatcttcg gtcatcgcaa caagattcac
     3301 atcatcaacc tggaaaagtc gctgccgatg ttccaggagg cgcagaagtt cgccaagcag
     3361 ctggccgcca accgcggcac catcctgatg gtgggcacca agcgccaggc ccgtgagctg
     3421 gtggccgagc aggcccagcg cgccggcgtg ccctacgttg accagcgctg gctgggcggc
     3481 atgctgacca acttcaagac cgtcaagacc tcgatcaagc gcctgaagga catgaaggcc
     3541 cagcaggaag ccggcctgga gtccatgagc aagaaagagc agctgatgtt cgcccgcgag
     3601 ctcgagaagc tggaaaagga catcggcggc attcaggaca tgaacgccct gcccgacgcc
     3661 atctttgtga tcgacgtggg cttccacaag atcgccatct ccgaggccaa gaagctgggc
     3721 attccgctga tcggcgtggt ggactccaac cacaaccccg agggcatcga ctacgtgatc
     3781 ccgggtaacg acgactcggc caaggccgtg gcgctgtacg cccgtggcat cgccgatgcc
     3841 atcctggaag gccgcgccaa cgccgtggat gaggtggtca aggcggttgc cgccgagggc
     3901 tccgacgaat tcgtggaagt ccaggactcc gccgcctaaa taccgggccg cgcgatgcgt
     3961 cgcaaaagaa gcggggcccc tgtgagcccc ctttttttta gcccttgttc cacttctaaa
     4021 tcacccgtgt cggcgttgct ttgccttgac aggcatgatt tggaaacgga attaatcttg
     4081 taacgaaccg aactgaaacc tggagaaata ccgatggctg ctattaccgc aagcatggtc
     4141 gcagaactgc gcgccaagac cgacgccccg atgatggaat gcaagaaggc cctgaccgag
     4201 gccgacggcg acatggccaa ggccgaggag ctgctgcgcg tgaagctggg caccaaggcc
     4261 ggcaaggccg cctcgcgtgt cacggccgaa ggcgtggtgg ctgccagcat cagcggcaac
     4321 ctgggtgcgc tgatcgaagt caacagcgaa accgacttcg tgtccaagaa cgacagcttc
     4381 atcgccatgg ccaatgccgc cgccaagctg gtggccgagc acaaccccgc caacatcgag
     4441 gccctgggcc aactggccta cgaacaagac ggcttcggcc cgacgctgga agacgtgcgc
     4501 aagggcctga tcggcaagat cggcgagaac atgtccttcc gccgcttcaa gcgctttgcc
     4561 ggcaacaacc tggcccacta cctgcacggc tcgcgcattg gcgtggtggt cgagtttgac
     4621 ggcgacgccg tggccgccaa ggacgtggcc atgcatgtgg ccgccatgaa gcccgtggcc
     4681 gtgaccagtg ccgaggtgcc tgccgatctg atcgagaagg agcgctcggt cgccgccgcc
     4741 aaggccgccg aatccggcaa gcccgccgac atcgccgcca agatggtgga aggttcggtg
     4801 cagaagtacc tcaaggaagt ctcgctggcc gaccaggtct tcgtgaaggc cgccgacggc
     4861 aagcagaccg tggccgccat gctcaagggc gccaacacca ccgtcaaggg cttcaccatg
     4921 tacgtggtgg gcgagggcat cgagaagaag accgacgact tcgccgccga agtggccgcg
     4981 caggtcgccg cagccaaggg cgcctgatcg cccttgcccc cgtcaccact ttcacccaac
     5041 ctcacggaga accagcccat gaccaacgcc gcaccagccc acaagcgcat cctgctcaag
     5101 ttgtctggcg aggcgctgat gggcgacgac gccttcggca tcaaccgcaa caccatcgtg
     5161 cgcatggtgc aggaggtggt cgatgtcacg cgcctggggg tgcaggtggc ggtggtgatt
     5221 ggcgggggca acatctttcg cggcgttgcc ggcggtgccg agggcatgga ccgcgccacg
     5281 gccgactaca tgggcatgct ggccacggtg atgaactccc tggccctggc ggacgccatg
     5341 aacaagcagg gcgtgaccgc gcgcgtgatg tcggccatcg acatccagca ggtggtcgag
     5401 ccctatgtgc gccccaaggc gctgcagtac ctggaagagg gcaaggtggt ggtgtttgcc
     5461 gccggcaccg gcaacccgtt cttcaccacc gacacggcgg ccgcgcttcg aggtgccgag
     5521 atcggcgccg agctggtgct caaggcgacc aaggtcgatg gcgtgtacac ggccgacccg
     5581 atgaaggatc cgacggccac gcgctacgcc aagctgacct tcgacgaggc catggtgcgc
     5641 aacctgggca tcatggatgc gacggcgttc gcgctgtgcc gcgaccagaa gctgccgatc
     5701 cgtgtgttct ccatcctgaa gcccggcgcg ctcaagcgcg tggtgatggg cgaggacgaa
     5761 ggcaccttgg tgtacgcttg agcgtttttc cgcgcagtac tgcccgctga aggatttcac
     5821 atgacgattg cagacatcaa gaagaccctg gacacgaaga tggaccagtc catcaccgcg
     5881 ctcaagaaca acctgtccaa ggtgcgtacg ggccgcgcca atccgcagct gctcgattcc
     5941 atccatgtcg agtactacgg ctccatggtg cccctgtcgc aggtggccaa cgtggccctg
     6001 ctggacgcgc gcaccatcag cgtgcagccc tgggaaaaga acatgggcgc caagatcgag
     6061 aaggccattc gcgagagcga cctgggcctg aacccggcgt cgctcggtga cctgatccgc
     6121 gtgcccatgc cgcccatgag cgaggagcgc cgcaaggaga tgaccaagct ggcgcgcaac
     6181 gagggcgaga actccaaggt ggccgtgcgc aacctgcgcc gcgacgccaa cgaggccgtg
     6241 aagaagctgg tcaaggacaa gctcgcctct gaggacgagc aaaagcgtgc cgaggccgac
     6301 atccagaaga ccaccgacaa gcacattgca gagatcgact ccctggtcgc ggccaaggag
     6361 caggacatcc tggcgatctg atggaaaagg ttgggcgttg agcgttcagc gtgcagcgcc
     6421 cgaaaccatg acgcacaacg caccacgtac aacgcctgac ggcggccacg ggccgcagca
     6481 tattgccatc gtcatggatg gcaatggccg ctgggccacc aagcgctttc tgccgcgcct
     6541 ggctgggcac cgccagggcg tggagtcgct gcgccgctgc gtgcgcgcct gtgtggcgcg
     6601 tggcgtgcgc gtgttgacgg tgtttgcgtt ctcctccgag aactggaacc gcccggcgga
     6661 cgaggtctcc ggcctgatgg agctgatggg caaggcgctg gtgcgcgagg tgccgcagct
     6721 caccaaggat ggcgtgcgcc tgcgcttcgt cggtgagcgc gccggcctgt cggcgggcat
     6781 gcgcgctgcg ctggagcagg ccgaggcggc taccgcggcc aacgtgcgca tgacgctcaa
     6841 cgtctgcttc aactacggcg gccgctggga catcgcccag gccgccgcca cgctggccgc
     6901 gcgcggcgag cccatcaccg aggccagcct gcacggcgcc atgggcatgt cccatgtgcc
     6961 cgaccccgac ctcttgatcc gcaccggcgg cgaaaagcgc atcagcaact ttctgctgtg
     7021 gcaggccgcc tattccgagc tgtacttcag cgagcgcctg tggcctgatt ttgacgaggc
     7081 cgccctggac gcggcgattg ccgactacgc cgcgcgcgag cgccgctttg gcctgacctc
     7141 cgagcaactg cagccggcgc aacccgaccc gctgcgcgcc tgaacggcgc ggtgcgccgc
     7201 attttcattt cgcgaaaggg cttggatgct caagcaacgt gtcatcaccg ccctggtttt
     7261 gctggccatc ctgctgccgg cgctgttcta tccctcgttc gtgccgtttg ccgtggtcac
     7321 gctggtgatg atggccgcgg gcgcctggga atggggccgc ctgaatggcc tgggccacgg
     7381 tgccagcctc gtgctgggcg cgctgtgcct ggccctgtgc tcgctgagct gggcggcggg
     7441 ctggctgcag cttgcgctgc cctggctgtg gacgctgggc ggcgccgcct gggtgctggg
     7501 cggtgcctgg ctgctgcgtg tgggtgtgga cggctggccg cgcgtgccgc gcgccctgcg
     7561 catcgtcggt ggcgtgctgg cactgtggtt ggcctggctg gccgtggccc aggcacgcgt
     7621 ggcaggcatc aactttctac tgtcggtgtt gaccctggtc tgggctgccg acatctttgc
     7681 ctactttgcc ggccgcgcct tcggactgcg cttcaccaag ggcaaactgg cgcccagcat
     7741 cagcccgggc aagagctggg agggtgtctg gggcggcatg gctggtgtgc tggcgctggc
     7801 tctggcttgg gtatgggctg acgcccactg gcagaccgcc gtgcccagcc tgtacaccca
     7861 cctggcgcgg cgcggctggt ggctgctgct gatcggcgcg ctgttcatgg cggcgatgag
     7921 cgtggttggc gacctggtgg agtcgctcat caagcgcagc gccggtgtca aggacagcag
     7981 cggcctgctg cccggccatg gcggcgtgct cgaccgcatc gatgcgcttt tgcccacgtt
     8041 gccactggcc atgatgcttt ctcactattc ctgacgcatg aagcaacgcc tgaccgtgct
     8101 cggatccacc ggatccatag gcaccaacac cctggacgtg gtggcgcgcc acccggatcg
     8161 ctacgaagtc tttgccctga gcgcggccac gcaggtggat ctgatgctgg cgcaatgcgc
     8221 gcaattttgc ccgcgctacg ccgtcatggc cagcgccccg catgcccgcc tgctggccga
     8281 aaggttaaag caaaatgacc tgtcaacgca ggtgttacaa gcgccagatg ctcttgaaac
     8341 aatagcgtca cacgaccagg tggacgccgt catggccgcc atcgtcggcg ccgccggcct
     8401 ggcgccctgt ctggcggcag cgcgcgccgg caaacgcctg ctgctggcca acaaggaggc
     8461 cctcgtcgtc ggtggcgcgg tcttcatggc ggcggtgcgc cagggcggcg ccacgctgct
     8521 gcccatagac agcgagcatt cggccatctt ccagggcctg cccgaggatg cgagtacctg
     8581 ggcgcggcgc gtcgatcaca tcctgctcac ggcctcgggc gggccgtttc gcacgcgcga
     8641 cccggccacg ctgcgcgccg tgacgataga ggaggcctgc gcccacccca acttctctat
     8701 ggggcgcaag atcaccatcg actcggccac catgatgaac aaggcgctgg aggtgataga
     8761 ggcgcgctgg ctgttcgacc tggcgcccga gcacatcaag gtggtcatcc acccgcagca
     8821 gatcatccat tcgatggtgc agttcaagga cgcttccatc attgcccagc tgggcacgcc
     8881 cgacatgcgc gtgccgattg cctgcggcct ggcctggcct gagcgcatcg ccagcggggc
     8941 tccgctgctg gacttcacgc agctggcggc gctgaccttc gaggaggcgg atgcgcgccg
     9001 cttccccggc ctgcacctgt cctggcaggc gctggccgcc gcccccggca ccacggcggt
     9061 gctgaatgcg gccaacgagg tggcggtgga ggcctttttg cagcagcgca tacgcttcga
     9121 tcagatccat gcgctgaatg ttgcaacttt ggcggccgtc cagccctcca atccggatac
     9181 gctgcacgcc ctgttggcac tggatgccga gacccgcgcc gtggcgcagg aactggcgca
     9241 gcgactcaat taagtgagct gccagcgctt gataaataag ggtttcaaat cgttttgatg
     9301 atggaatcgt tgtttatgaa gcgccagcag ctatgttttt ttaaggaagc cccagcatgc
     9361 tcaccatcgc cgcctttatc gtcgcactgg gcgtactgat agccgtgcac gagtacggcc
     9421 actaccgcgt ggcggttgcc tgcggcgtga aggtgctgcg cttctcggtg ggctttggcc
     9481 ggccgctgct gcgctggcag cgcaagggtt cgccgacaga gtttgtcatc ggtgcgtttc
     9541 cgctcggcgg ctatgtgcgc atgctcgacg agcgcgaggc acccgtcgcc gctgaagatc
     9601 gccatctggc cttcaacacc cagccgctgc gcgcgcgcgc ggccatcgtc gccgcggggc
     9661 cgctggccaa cctgctgctg gctgtgctgc tgtatgccac cgtcaactgg agtggggtgg
     9721 acgagcccaa ggccgtgctg gccagcccgg tggccggctc catcgcgcag caggcgggtt
     9781 tgcacggggg cgagctggtg cttggcgccg ccctgggcga gggtgacttc gagcccgtgc
     9841 gctcgtttga ggatctgcgc tggacgctca cgcgtggcgc cctcgatggc gagagcgtgc
     9901 gcctgcagtt ccagccacag cgtggctcgg cccagcgcga gctggtgctg ccgctggaca
     9961 agctggaggt gcgcgaggcc gatgcacacc tgtttcgcag gattggcatc gtcggcccct
    10021 ggacgcgccc ggtgctgggc gagctggtgg ccggtggcgc ggccgaacgc gccggcctgc
    10081 gcgccggtga cctggtgctg cgcgttggct ccggcgatgt ggttgacggc agccagctgc
    10141 gcgagctgat ccgcaccagc gggcgcggcg gccagccgca ggcactggcc tggcgcgtcg
    10201 agcgcgatgg ccggcagctc gatctgttgg tgcaggccga tgtggtgcag gaggccagtg
    10261 gctccgtggg gcgcataggc gcctatgtgg gcgcagcgcc cgagatggtg aacgtgcgct
    10321 acggcgccac cgagggcctg tggaagggcg ccgagcgcac ctgggaggtg tcggtgctca
    10381 cgctgcgcat gctgggccgc atggtgatcg gcgaggcttc ggtcaagaac ctcagcgggc
    10441 cgctgaccat tgccgactat gcgggccgct cggccagcat ggggttgacg caatacctgg
    10501 cgtttctggc cctcatcagc gtcagcctgg gggtgctgaa cctgctgccg ctgccggtct
    10561 tggacggtgg gcacctgatg tattatcttt gggaggctct gaccgggcgc agcgtatccg
    10621 acgcctggat ggaacggctg cagcgcgggg gcgtcgcggt gctcctgctg atgatgtcca
    10681 ttgccctgtt caacgatctc acccggctat ttggctaact ttttgccgct gctgcgcggt
    10741 agcggcggct tcagcttcac tcatgagaaa acacatcaat cgcctgggcg tgcgcaccgc
    10801 atcggccctt gtggccatgg cctttgccac ccaagccgcc tgggcgctgg cgcccttcaa
    10861 ggtgcaggac atccgcgtcg agggcctgca acgcgtcgag cccggcacca tcttcgcgtc
    10921 gctgccgctg cgcgtgggtg acgagtacaa cgacgagaag ggcgccgccg cgatccgcgc
    10981 gctgtttgcc ctgggcctgt tcaaggacgt gcgcctggag gccaccggca atgtgctggt
    11041 ggtggtggtc gaggagcgtc cgacgatcgc cgacgtcgaa tttgccggca ccaaggagtt
    11101 cgacaaggac accctgaaga aggccatgcg cgacgtgggc ctgaccgaag gccgcccgtt
    11161 cgacaaggcc ctggcggatc gcgccgagca ggagctcaag cgccagtaca tcaacaagag
    11221 cctgtatggc gccgaggtcg tgaccaccgt cacgccgata gagcgcaacc gtgtcaacct
    11281 gaccttcacg gtgaccgagg gcgaacccgc caaaatcaag gacatccgca tcgtcggcaa
    11341 ccaggccttc agcgaatcca cgctcaaggg cctgttcgac caggacacgg gtggctggat
    11401 gagctggtac accaagagca accgctacgc gcgcgccaag ctcaatgcgg atctggagac
    11461 gctgcgctcc tactacctgg cgcgcggcta cctggagttc cgcatcgact ccacgcaggt
    11521 agccatctcc ccggacaagc aggacatctc catcaccatc aacgtcaccg agggccagcg
    11581 ctacgtcgtc tcgggtgtga agctggaggg cagctatctc gaccgtgacg acgagttcaa
    11641 gtccctgatc accatccgcc ccggcgagcc ctacaacgcc gacaaggtgg cggaaaccac
    11701 caaggccttc accgaccatt ttggcaactt cggctttgcc ttcgcgcgcg tcgaggccgt
    11761 gcccgagatc gaccgcgaga acaaccgcgt ggccctggtg ctgcgcgccg agcccgcgcg
    11821 ccgcgcctat gtgcgccgca tccaggtcag cggcaacaac cgcacgcgcg acgccgtggt
    11881 gcgccgcgag ttccgccagt acgaggcctc ctggtacgac ggcgacaaga tcaagctgtc
    11941 gcgcgaccgc gtggaccgcc tgggcttctt caccgaggtc aacgtcgaga cgcaggaggt
    12001 gcccggctcg cccgaccagg tggacctggt catcaacgtg gtcgaaaagc ccaccggctc
    12061 gctgcagctg ggcgcgggtt tttccagcgc cgagaaggtc tcgctgtcgt tcggcatcaa
    12121 gcaggaaaac gtctttggct cgggcaacta cctgggcgtg gatgtgaaca ccagtaagta
    12181 ccgccgcacg ctggtgttct ccaccaccga cccgtatttc acgcaggacg gtatctcgcg
    12241 cacgctggat ctgtactacc gcacggccaa gccctaccag caccagggcg gcgactacga
    12301 actggtcacg gccggcgcca gcgtgcgctt tggcgtgccg ttcagcgaga acgacaccgt
    12361 gtttttcggt ggcggcctgg agcagacaca gatcaagtcc ggcaccaaca tccctgccgc
    12421 ctatctggcc tatgcacgcg aatttggcga caccagctgg tcgctgccgc tgaccatcgg
    12481 ctggtcacgc gacaaccgcg acagcgcgct ggcgcccaac tccggccgct accagcggct
    12541 gaataccgag tgggccgtgg ctggcgacgc gcgctacatc cgcggcaact accagtacca
    12601 gcaatacatt ccgctgaaca agcaatacac ctttgccttc aatggcgagc tgggcctggg
    12661 caagggcatg aatggccagc ccttcccggt gttcaagaac ttctactcgg gcggcctggg
    12721 ctcggtgcgc ggtttcgacc agggtacgct gggcccgcgt gacgtgaccg gcgcctcgct
    12781 gggcggcccg aagaaagtca cgctgaacgc cgagctgatt gcgccgttcc ccggcgccgg
    12841 caatgaccgc acgctgcgct ggtttgcctt cgtcgatgcc ggcaacgtct acggtgaaaa
    12901 tgacaactgg gagctgagcg agctgcgcgc ttccgcgggc ctgggcctga gctggatttc
    12961 tccgctgggc ccgctgcgtt tggcctatgc gcagccggtg cgcaagtttg ccggcgatag
    13021 aatccagaaa ctgcaattcc aaatcggaac gtccttctaa tgaaatctct ttcccgccat
    13081 attcccctgg tttttctgct gggttccctg gccgctgccg tgcctgccca ggcccaggag
    13141 ttcaaggccg gcttcgtcaa taccgaccgt atcttccgcg aggccaacac cgccaagacg
    13201 gcgcaggcca agctggagca ggagttctcc aagcgcgaga aagagctcgt ggacatgggc
    13261 aatgccctga agagcgctac cgagaaattc gagcgcgagg cccccaccat ggccgagagc
    13321 cagcgcgtga cgcgccagcg ccagctggtc gatcaggatc gtgacttcca gcgcaagcgc
    13381 cgcgagttcc aggaagacct gggcgcgcgc aagaacgaag aactgggcca ggtgctcgag
    13441 cgcgccaaca aggtcgtcaa gcagttggcc gaggccgaga agtacgacgt catcctgcag
    13501 gaagcggtgt acatcaaccc caagcacgac atcaccgaca aggtgatcaa ggccatgaac
    13561 gctgccggca actccggcaa gtaaagcgcg ggcgcaccaa tacaggccag gcgtgagttt
    13621 gcggctcggg caaatcgtcg atgcactcgg gggcagcctt gaaggagcca gcgcagacac
    13681 gcaaatccag cgcatagcac cgctggagac ggccggtgag ggcgacctgg cctttctgaa
    13741 caacccgcgc tatcagtcgc agctggccgc ctcacgggcg gcctgcgtga ttgtggcgcc
    13801 ggccatgcgc gccgcggcgc tggcgcgcgg cgcctgcatc gtcaccgatg acccctatgc
    13861 gtactttgcc cgcgtcaccc agctgtgggt gcgcgcgcaa ggccaggccc cggtgcccgg
    13921 cattcacgcc agtgcggtgg tcgatgcaca ggcgcaggtg catccaacgg ccacgattgg
    13981 cccgctgtgc gtggtcgagc gcggggccgt gattggtgcc catacggtgc tcaagtcccg
    14041 tgtgacggtg gccgagcgct gcaccgttgg cgcgcgctgc atcctgcatc cgggggtggt
    14101 catcggcgcc gacggctttg gctttgcgcg ccagcagggc gagtggatca agatcgagca
    14161 gctgggcgcc gtgcgcattg gcgacgatgt ggagataggc gccaacacct gcatagaccg
    14221 cggcgcactc gaggacacgg tgattgagga cggcgtcaag ctggacaacc tgatccagat
    14281 cgcgcacaac gtgcacatcg gtcggcacac cgccatggcg ggctgctcgg ccgtggccgg
    14341 cagcacgcgc ataggggcgc attgcaccat tgcgggggcg gcgtccatcg tcgggcacct
    14401 gcaattggcg gacaatgtgc atgtctccac caacacggtg gtcacgcatt cgattgcacg
    14461 gcccgggcag tacaccgggg tgttccccat ggacgaaaat tccaagtggg aaaagaacgc
    14521 ggccacgctg cggcagctgt acaagctgcg cgaacgcatc aaggcccttg aacaagcacg
    14581 acaaggcgac cagagcgcta cacaacaatg atggacattc acgcaatcct caagcaactg
    14641 ccgcaccgct atcccttcct gctggtggac agggtggtgg agctcgagcg caacacgcgc
    14701 atccgggcca tcaagaacgt caccttcaac gaaccatact tcaccggcca tttcccgggc
    14761 cgcccggtca tgcccggcgt gctgattctt gaggcgctgg cccaggcggc cggcctgctg
    14821 gccttcgacg ccatgggcca ggtgcccgac gagaacaaca tctactactt tgtcggcatc
    14881 gacggcgcgc gcttcaagcg cccggtggag ccgggcgatc agctgatcct cgaaatcacc
    14941 atcgaccgcg tgcgcggcgg tgtctggaag ttcaacggcg tggcccgtgt gggcgatgag
    15001 gtggcctgcg aggcgcagct catgtgcacc atgcgcaacg tcggcggctg atgcagcagc
    15061 ccgtgagcca gatccacgct acggccatcg tcgcggccgg ggcgcagatc gaccccagcg
    15121 ccacggtggg gccgtacgcc atcatcggcg ccaacgtgcg cattggtgcg cgcaccagcg
    15181 tcgggccgca ctgcgtgatc gagggccaca cgacgatagg cgcggacaac cacatcttcc
    15241 agttcgcctc gctgggcgcc gcgccgcagg acaagaagta cgccggcgag ccgacgcgcc
    15301 tggagattgg cgaccgcaac accatccgcg agttctgtac cttcaacacc ggcacggtgc
    15361 aggatcaggg tgtgacccgt atcggtgacg acaactggat catggcctat gtgcacattg
    15421 cgcacgactg cgtggtgggc aaccaggtga tattggccaa caacgccacg ctggccggcc
    15481 atgtgcaggt cggcgaccag gtcatcatcg gcgggctgac gggggtgcac cagtattccc
    15541 gcataggcgc gcatgccatg gcgggctttg ccagccatat ctctcaggat gtgccgccct
    15601 tcatgatggt ggacggcaac ccgctggccg tgcgcggtct gaacctggaa gggctgcgcc
    15661 ggcgcggctt ctcggcgcag cgcgtggccg ccatcaagca ggcctaccgc ctgctgtacc
    15721 gccagggcct gacgctggag gcggcactct cggccatggc cgagctgccg cagcagcacc
    15781 ctgaggccga tggcgacatc gccctgctgc gcgacttcat cgccgcatcg cagcgcggca
    15841 ttgcgcgctg atattttttc cggcgcaatg gctggcatct ctccccgcgt tgccatggtg
    15901 gccggtgaaa cctcgggcga cctgctggcc ggcctgctgc tcgacggcct gcgcgcccag
    15961 tggcccggtg tggccagcat gggcataggc gggccacgca tggctgagcg tggttttgac
    16021 gcctggtggc ccagcgaacg cctggccgtg catggctaca gcatcgagct ggtgcggcgc
    16081 ctgctgggca tcctgaagat ccgcaaacaa ctgcgcacgc gcctgctggc cgacaggccc
    16141 gatgtgttca taggcgtgga tgcgcccgac ttcaacctgg gcctggaggc cgatctgcgc
    16201 aactccggcg tgaagaccgt gcattttgtc tgcccgtcga tctgggcctg gcgcgccaac
    16261 cgcgtggaca agatccgcgc cgcggccgac catgtgctgt gcatctttcc attcgagccc
    16321 gagctgctgg cgcgccatgg catagccgcc acctatgtgg ggcacccgct ggccagcgtc
    16381 atccccatgc agccggacaa gctggccgca cgcgcccagc tgggtctggc accgcaggac
    16441 gaggtgctgg cgatactgcc cggcagccga tcggccgagg tcgcctacat cgcaaaacca
    16501 ttttttcagg ccgcagcgct tatcagtaaa gcgcggaatg ctatcaaaat agtagtccct
    16561 gcggtgcccg cactgcgtgc gcgcatcgag cagatcgcgc gcgactgcgg cgtgctggag
    16621 cagctgcaga ttgtcaccgg ccagtcgcac caggtgctgg ccgcctgcga cgtgacgctg
    16681 atcgccagcg gcacggccac gctggaggcg gcgctgttca agcgccccat ggtcattgcc
    16741 taccacatgc accccatcag ctggcgcctg atgcgtggca agcagctgca gccctgggtg
    16801 ggcctgccca acatcctgtg ccgcgacttc gtcgtgcccg agctgctgca ggacgctgcc
    16861 acgcccgagg ccctgtcggg tgcggtgcag cagtggctgg acgcgccgcc ggcgcgcctg
    16921 gacgcgctgc atcaacgttt caccgcgctg catgaacagc tgcaacgcga cacgccacga
    16981 ctggccgcca atgccatcca agaaatcctg gcttgagcag ggcaacctca actggcatgc
    17041 gcccggcctg ttggttgccg gcgtggacga ggccggccgc ggcccgctgg ccggccccgt
    17101 ggtcgccgcc gccgtcatcc tggacgaact gcagcccatt gccggcctgg ccgactccaa
    17161 ggccctcacg gcggcgcgcc gcgagcggct gttcgatgag atccgcgcca aggccctttg
    17221 ctgcagcatt gccgaagcca gcgtggaaga gatagaccgg ctcaacattc tgcaggccac
    17281 catgctggcc atgcgccgcg ccgtacaggg cctgcgcctc aaaccccgtc tggcgctggt
    17341 ggacggcaac cggctgcctg tgctggatgt tccggccgag gccgtcgtca agggcgatgc
    17401 caaggtggcc gccatctcgg ccgcctccat cgtcgccaag gtcacgcgcg accgctggtg
    17461 tgcccagctg cacgaccaat atccgcaata cggctttgcc ggccacaagg gctacggcac
    17521 ggccgaacac ctggcagccc tgcaggcgca tggcgcctgc ccgcagcacc gccgctcctt
    17581 ttcccccgtg gcgcgcgccc tgcaggctgc gcccgcacaa ccatgacgcg ccagcagccc
    17641 accctcatcc agtcgcgtga caacccgttg ctcaaggatc tgcggcgcct ggccctggac
    17701 agtacggcct accgcaagca gggtagggtg tggctggagg gcgaccatct gtgcggcgcc
    17761 gccctggtgc gtggccagcg tccggccatt gcggtgtttt ctgaaagttt ttggcctctt
    17821 gcgcccgctg aatatgcgca agcagctatt aaaacaatag tgatgcccga tgccctgtgg
    17881 cgggacatca gcggcctcga atcacctgcg cgcatgggct tcgtgctgcc gctgccggtc
    17941 gagcaggggc tggatgcgca agcgcccacg gtggtgctgg atcgcctgca ggatgcgggc
    18001 aacgtgggct ccatactgcg cagcgcctcg gccttcggct tcacgcagat cgccgcgctc
    18061 aagggcacgg cggccctgtg gtcggccaag gtgctgcgcg ccggcatggg tgcgcatttt
    18121 gcgctgcgcc tgctggaagg cctggagctg caggacatcg accgcctggc cgtgccgctg
    18181 ctggccacca gctcgcacca gggcgactgg ctgcaccgtg ccgccttgcc ctggccctgc
    18241 gcctggatca tgggccacga agggcagggc gtttcacccg agctgcagct gcgcgcacgc
    18301 cagcacatcc gcatcaccca accgggcggc gaggagtcgc tcaacgtcgg cgccgccgcc
    18361 gccatctgcc tgcatgccag cgctgcggcg cgtggctgaa cggcccggca agtacgccaa
    18421 accaatgatt gacgccctga tatccggcaa gctgcacgcc caccctgagc agcgcaccag
    18481 caagaccggt aaaacctatg ccacggccaa gatgacggcc gccggtggcg acggccaaag
    18541 cctgttcgtc aactgcattg ccttcgacga ggcgtcggtg gaagcgctgc tggcgctggt
    18601 tgccggggac agcctggccg tggccggcag catcaccccc aaggtctgga ccgatcgcga
    18661 gggccaacac cgcccggcgc tggacatggt ggtcgcgcgc gtgctgaccg cctaccacgt
    18721 cacgcacaag cgccgtgcca tgcagcaaag cgccgctcca caggcgcggc agcagcccat
    18781 ggacgatggc gagccactgg acttctgacg gcaggcagca aagcaaaaag gccctgattc
    18841 ttgcgaatca gggcctttca tttggtgccg gagacatgaa tcgaacacgc gaccttctca
    18901 ttacgaatga gctgctctac cgactgagct acaccggcta aagacggcga ttatataccg
    18961 ttgtggtagc tggttacgcg ttcaacttcg ttcttggagc ccaggatcac gctcacgcgc
    19021 tcgtgcagct gcgtgggctt gatgtccagg atgcgctgtt tgccgttggt ggcggcgccg
    19081 ccggcctgct ccaccagcca gcccatgggg ttggcctcgt acatcaggcg cagcttgccg
    19141 ggcttgttgg gttcgcgctt gtcccagggg tacatgaaga tgccaccgcg gctcaggatg
    19201 cggtgcacgt cggccaccat gctggccacc cagcgcatgt tgaagtcctt ctcgcgcggg
    19261 ccttccttgc cggccaggca ttcgtcgatg tagcgcttca ccggttcgtc ccagtggcgc
    19321 atgttgctca tgttgatggc gaattccttg gtgtcctcgg ggatgcgcac gttctcgcgg
    19381 atgagcacga aggagccctg ttcgcggtcc agcgtgaaca tggcgacgcc gtcgccaacg
    19441 gtcagcacca gggtggtctg cgggccgtag atgcagtagc cggccgcgac ctggcaggtg
    19501 ccgggctgaa ggaagtcgtt gtcgtgcacg ccggggtggc cctcgggctt cttcagcacg
    19561 ctgaagatgg tgccgatgct gacgttgacg tcgatgttgg acgaaccatc gagcgggtcg
    19621 aacatcagca ggtattcgcc ctgcggatag cggttgggca cgacgtagat gccctccatc
    19681 tcctcgctgg ccatggccgc caggtggccg ccccattcgt tggcctcgat cagggtttcg
    19741 ttggcgatga tgtccagctt cttctgcacc tcgccctgca cgttctggct gccggcggtg
    19801 cccatcacgt cgcccagttc gcccttgttg acggcctgtg agatgcgttt gcaggcgcgc
    19861 gccaccactt ccagcagcag gcgcagctgg ggcgggatgc ggccatcgac gcgctgttgt
    19921 tcgacgaggt agcgggtcag gctgatgcgt tcggtcatgg gtgggtcctt ttccttttca
    19981 atcaatcggc gagagcgcgc gtgacgactt cacgcacatc gttggacagg tccgccttgg
    20041 cggcgacgcg ggcgatggcc tcgcgtgcgg ccgtgcggta gggctcggcc agcttgctcc
    20101 agcgatcgag tgcgcgcgcc aggcgggccg cgacctgggg gttgagggcg tccagctcca
    20161 gcacgcgctc ggcccagaag acgtagccgg cggcgtcgcg ccggtggaag gcaccggggt
    20221 tggcgctgca gtagctaaag atcacgctgc gcgcgcggtt cgggttcttg atgtggaagt
    20281 cgggatgctt cagcaaagcc ttgacggcgg gcagcacgtt gccgccgcgg tcggtgctgc
    20341 cggcctgcag ggcaaaccat ttgtccagca ccagggcgtc gtctttgaac agtgcatgaa
    20401 agcgcgccag cgccggctgg gccagctcgt ggccgctcat caccagggcc gacagcgcgt
    20461 tgaagcggtc ggtcatgttg gcagcaatct tgaagcgctg gtaggtcttg ccgggccaca
    20521 cctcgtcgcc cgtggcgcgc gcggccaggc acagcatggc cagggccagg ccggccaggg
    20581 cgcgccggcc gctgctggtg gcgtcggggc ggtaggcgcc gctggcctgg tgctcgtccc
    20641 aggcccaggc ccagtcggcc tgcagggaca gcgccagctg caggcgcagg gcctcgcgca
    20701 cggcatgcac gcgctgcgga tcgacttgcg tcagctgctc ggcgatgtag ctctcggagg
    20761 gcagggtcag caccagctcc ttgaaggccg cgtccagctg cgggtggcgc agcacggcgc
    20821 gcatggcctg caccagggcg tcgggcaaaa ggttttggtc tatttggccg tttgcgccct
    20881 ctgttattgt gctgtcagct atttcattga tagcaatacg cagcatcagg cgctggccgg
    20941 cctcccagcg gttgaaggcg tcgccgtcat gcgccagcag ggtcagcagt tcggtgtcgc
    21001 tgtagtcata gtccagcacc accggggcgc tgaaaccgcg taacagcgag ggcacgggct
    21061 ggctggtgac gttgacgaag cccagcgtca ggctctcctc ggtcagcacc acggtgcgtt
    21121 cggtgctgcc tgccgtggcc tcgccatcga gttgcagggg cagggcgttg ccctgcgcgt
    21181 ccagcaggcc cagtgccacg ggaatgacga agggcagctt gttcggctgg cccggtgtgg
    21241 cggcgcagct ttgcgacagg gtcagggtgt agcggccggc gtcggcgtcg tactggccca
    21301 cggccttgac atggggcgtg ccggcctggc tgtaccagcg cttgaactgc tccaggtgca
    21361 gggccagcgg gctggccggg ttggcgtcgg caatcgcctg ggcaaagtcg tcgcaggtga
    21421 cggcctggcc gtcgtggcgc tcaaagtaca gcttcatgcc gcgggcaaag ccttcgcggc
    21481 ccaccaggcg gtgctgcatg cgcacgacct cggcaccctt ttcgtagatg gtgacggtgt
    21541 agaagttgtt gatctcgacg tagctgtcgg ggcgcaccgg gtgggccatg gggccggcgt
    21601 cctcggggaa ctgcgcggtg cgcagcacgc gtacgtcctc gatgcgtttg acggcgcgcg
    21661 ccgacgggct gcccgccatg tcctggctga actcctggtc gcggaacacc gtcaggccct
    21721 ctttcagcga gagctggaac cagtcgcggc aggtgacgcg gttgcccgtc cagttgtgga
    21781 agtactcgtg gccgacgacg gattcgatat tggcgaagtc ggtgtcggtg gccgtggcct
    21841 cgctggccag cacgtacttg gtgttgaaga tgttcaggcc cttgttctcc atggcgccca
    21901 tgttgaagtc gctggtggcg acgatcatga agcgctccag atccagcgtg aggccaaagc
    21961 gcgcctcgtc ccaggcgatg gacgccatca gcgagttcat ggcgtgctcg gtcttgccca
    22021 ggtcgcccgg gcgcacccag acctgcagca ggtgcttgtt gccggcgcgg ctggtgacct
    22081 gctgctcgcg cgccaccagc ttgccggcca ccagggcgaa caaatagctg ggctttttgt
    22141 gcgggtcttg ccagcgggcg aagtggcggc cgtcttcgag ctcgccggac tgcaccaggt
    22201 tgccgttgga gagcagcacc ggatagtcgg ccttgctcgc gcgcagcagc acctggtagc
    22261 tggccatgac gtcgggacga tccaggaagt aggtgatgcg ccgaaagccc tcggcctcgc
    22321 actgcgtgaa gaaggtgccc tggctcacgt acaggcccga cagctgcgtg ttcttggcgg
    22381 gcgcgcaggt ggtgaagatc tccagcgcga agggcgcgtc gccctccggc aggttctcca
    22441 gcaccagctc gtcgccctcg atcttgaacg aggtgcccgc gccattgacc agcacgcgcg
    22501 ccaggttcag ctcctggccc agcaggcgca gggcctgggg cgcggcggcg ctgttgcggc
    22561 gcacctgcat cttgttcagg acgcgggtct tggccgggtc caggtcgaac gtcagatcca
    22621 cgctgtcgat ccagaacgcg ggcggggcgt agtccaggcg ctcaatggcc ttggcttgtc
    22681 cttctttcat cgtgcttgtc cttctgtttt acaggccttg cttgagcgag gcctcgatga
    22741 atgcatccag gtcaccgtcg agcacctttt gcgtggccga tatttcgacg ttggtgcgca
    22801 ggtccttgat gcggctgttg tccagcacat agctgcggat ctggtggccc cagccgacgt
    22861 cggtcttggt ctcctccagg gcctgctgcg cctccatgcg cttttgcatc tccagctcat
    22921 agagcttgga gcgcaggcgc tgccaggcca cgtcgcggtt gctgtgctgg ctgcgtccgt
    22981 cctggcactg cacgacgatg ccggtgggca tgtgcgtcag gcgcacggcc gaatccgtct
    23041 tgttgatgtg ctggccgccg gcgccgctgg cgcgataggt gtcggtgcgc acgtcggcgg
    23101 ggttgatgtc gatctcaatc gagtcgtcga tctcgggata gacaaacagg ctggcaaacg
    23161 aggtgtggcg cccgcccgag ctgtcgaacg gcgatttgcg caccaggcgg tgcacgccgg
    23221 tctcggtgcg cagcaggcca taggcgtagt cgccctcgat gtggatggtg gcgctcttga
    23281 tgccggccac gtcgcccggg gtttcctcgt cgaccgtggc cttgaagccc ttcctctcgg
    23341 cgtacttcag gtactggcgc agcagcatgc tggcccagtc gcaggcctcg gtgccgccgg
    23401 cgccggcctg gatgtcgatg tagcaattga gcgggtcggc ctcgcgcccg aacatgcggc
    23461 ggaactccag ctcctcgatc agcggcagca gcttggcggt ctcggcctct atggtgagca
    23521 gcccggcttc gtcgtcttcc tcgtagctca tctcgtagag ctcgcggttg tcggccagct
    23581 cggccgtcag cttttgcagc gtcagcacca cggcgtcgag ggacttcttt tccttgccca
    23641 gttcctgggc cttcttcggg tcgttccagc aggccgggtc ttccagcgag gcgtttaccg
    23701 tgcgcaggcg ttcgtacttg gcatcgtagt caaagatacc tccgtagctc ggccgtgcga
    23761 gcgctcaggt cttcgagggt gttgccaatc tggttgatgt gttctgcttc catggtgcgg
    23821 gtgtcctgtg tgtttctttg gtgataaccc gcgattttct catgacgcgt gatggcgccc
    23881 gtggctgccg gacttggcgc cacgcagggg cggggtatgg tttgctgtaa ctatcaaaaa
    23941 aagagctaat gacgcttgat gtacaagcgc cagagccatt tttgactgat attgtctggg
    24001 tttacagcag ccgctcacgc agctcggccg ggctttgcgg cgacagcagg ccgggggcca
    24061 cgtcgaacac cgtcttgcag ccgaactgcc cggcctgggc catgcggtgc acggcgcgtg
    24121 cgtaggccac cagcacgctg gaggtgaatt cggggttgct atcgagctgc agccggtatt
    24181 cgatgacctg cttgttggcg gccgaggtgt tgccgctgcg gatcacgaag ccgccgtgcg
    24241 gcatcgcgcc atgctcgcgc gccagctgct cggcgctgat gaactgcacc gtggtgtcgt
    24301 actcgtcaaa gtagtgtggc atggtgacga tggcctggcg tacggcctcg gcgtccgccc
    24361 ccggggccag caccacgaag cagtcgcgcc ggtgcttctc gcgcgtggtc agctgcgggc
    24421 gcacgccgct gcgcacctgg tccacggcct cgggcacggg gatggtgtat tgcacgccac
    24481 cggccacgcc gctgacgcgg cggatggcgt cggaatgccc ctggctcagg cccttgcccc
    24541 agaaggtgta ggtggcgccg tcgggcagca gggcctcgcc cagcacgcgg ttgatggaga
    24601 acatgcccgg gtcccagccg gccgagatca gcgccgtggt cttgcccgcc tgcgcggcgg
    24661 cgtcgacggc ggcgaaatgc gcggggatgc gcgcatgggt gtcaaagctg tccaccaggt
    24721 tgaactgcgc cgccagctcc gggctttggc gcggcaggtc gtccttggag ccaccgcaga
    24781 ggatgagcac gtcgatcctg tccttgtggc cggccagcgc gtccatgggg tacacggaca
    24841 cgcccgcggt caggggctgg agctgcgccg ggtcgcggcg cgtgtagatg ccggcaatgg
    24901 tcatgtcggg gttgttggcg atggccgact ccacgccacg gcccaggttg ccgtggccga
    24961 cgatggcgat gcgtatggct ggctgcttgt cgttcatggg tgtttgcttt ctgtttgctt
    25021 caaaaatagt agctgtatgc gcttgttggg tgagcgcaaa ccggctgttt tgcttaattt
    25081 tttggccgcc tcggcgcagc ccgaggcggc attgtcaggc aggcatcttg tctgccagaa
    25141 aatcctcgat cagcgccgcc acggcctggg gctggtcgtg gtgcagcata tggccggcgt
    25201 cctgcaccag cgcgctgcgg ctgtcggcca catgggccag gcgctggtgg tagtcctgca
    25261 gcgtgtagcg gcccttccac cagcggccca ggctgtcgtc gctggcatgc accgagagca
    25321 cgggggcggt gatggccgcg tacagcgcca gagcctcgtc cacgcggtac aggtgcgggt
    25381 tgacgatctt gtgcgccgcg tcgcccagga tatgccagcg cccctgcgcg tccgcgccgg
    25441 cccagtggcg cgccagccag caggccttgt cgctggccag acgcgggttg gtcttcatca
    25501 ggcgcagggc cacgccgtcg gcgctgtcgt agggtttcag gtccatctcg ccgcgctccc
    25561 attgccgaat ctcgtccatc cactgcgcat agcgcccggg ggcctgggcg gggttggagg
    25621 gggccatgcc aaagccctcc aggttcacca gtcggcgtat gcgcgccggc cgcgcgccgg
    25681 catacatcat ggccacgttg ccgcccatgc tgtggcccac caggtccacc ggctgcgcgc
    25741 ccgcatagtg gtccagcagc tgatccagat ccgcaagata gtccacgaaa tggtggccgt
    25801 cacagggcgc ggccaggcgt gagccgccaa agccgcgcca gtcgggggcg atgatgaggc
    25861 gcccggcggc aaaggcctcg ccgaaggcat cgaccacgaa ctgccaggac gcgcccacat
    25921 ccatccagcc atgcagcagc agcagcggcg ccgtgcccgg ctgcggcgcg ccccaggtgc
    25981 gtacgtggta gttcagcgtg cggatgcgca cgctgtcgct gcggctgggg cgtagggctt
    26041 ggtacatggg ctctggacag tggttgcgtg gccggttgca cggggagagg gggctgccgc
    26101 gcaaaccatg ataggcaggc gcgccgcccg ggttatacag tgccggcaca tccatcacga
    26161 taaggaggca gacatgcagc gcagcattgt ggcagcggca ttggcggcag gtttttgtgg
    26221 gctggcgcta ccggccattg cgggccaggt gacggtgatc acgtccttcc ccaaggagct
    26281 gaccacggcc taccagaagg ccttcgaggc cgcccacccg ggcatcaaga tcgagatcct
    26341 gaacaagaac accacggccg ccgtggccta catccgcgag ctgcccgagg gccagcgccc
    26401 cgacgtgatg tgggccagcg cacccgacgc cttcgaggtg ctggccagct accagctgct
    26461 gcaaaaggcg cccgaggtgg tcaacaaaag cgcgccggag aagataggca actacccgct
    26521 caacgacccc aagggcatgt actacggcca ggcgctggcg ggctacggca tctcgtggaa
    26581 cacgcgctac atcaaggcgc acaagctggc ggcgcccagg gagtggagcg acctgacgcg
    26641 gcccgagtac ttcggccatg tcgccatctc gtcgccctcg cgctcgggca cgacgcagct
    26701 cacggtggag accattttgc agggcgaggg ctgggacaag ggctggtcgc agctcttgca
    26761 gatcatgggc aacgccgccg ccgtcaccga ccgcagcttt gccgtgcccg acggcgtgaa
    26821 caacggccag tacggcatag gcatcgtgat cgacttcctg gcgctggcgg gcaagtactc
    26881 gggctatccg gtggacttca cctaccccag catgacggcc gtggtgccgg ccaatatcgc
    26941 cctggtcgcc ggcgccaaga acgcggccga ggccaagcag ttcatggcct acaccatgtc
    27001 ggcggcgggc cagcagattc tgttcgaccc caagatcggc cgcctgccga tactgccgta
    27061 ctccatgctc aaggcccccg caggctaccc cgtgccgcag gacatcgcca agcgcgccaa
    27121 ggtgcagttc aacaccgagc tgtcgggcca gcgctacccc gtggtcatca gcctgttcga
    27181 ccagatggtg accttccgcc tcaaggagct gcaggccgcg accaagtcca tccacgaggc
    27241 cacggccgcg ctcaaggcca ggcctaacgc ccggggcagc gagctactag cgcaggcgcg
    27301 cagcctggcc tatacgccgc tggtgggcgc ggataacgtg aagaaccccg agttcctgga
    27361 gctgtttcgc aagagccggc gcgacgtggc cgtggccaag gagctgacgg gcatggagca
    27421 gctgtggtcg gagaaggcgc gcgccaacta tgtgcgtgcc aagcagctgg cggacgaagc
    27481 gcgcggcctg gcccgataaa tccattattc aagaccgcca catctgccgc tctgggcggc
    27541 gggtgtgatc gaacgccatg cccgttgctc acctatcgac cgccgcgcgt gcgccgcgcg
    27601 catctgccgg cctggggccc tggatcgcct ttgcgctgat cctggccttc ttgctggtgt
    27661 ttctgatcct gcccgtgggc acggtgatct acaccgcttt cgtcaccgac accggcgcgc
    27721 tgaccgtggg ccattttggc aattttttcg accagctggt gtttcgcgag tccttcttca
    27781 acagcctgtg ggtggcactg gccagcacct tttttgccag cgtcatcgcg ctgccgctgg
    27841 cctacctgac ggtgcgcttc gagttccgcg gtgcgctgtt gatacagaca ctgggcgtgc
    27901 tgccgctgat catgccgccc ttcgtcggcg cggtggcgtt gcagctgatc tttggccgca
    27961 gcggctcggt caacctgctt ctcaatgact ggttcgacat caccatcccc atcatggatg
    28021 gcctggtggg cgtgaccttc gtcgagagca tccactactt ccccttcatc ctgctgaacc
    28081 tggtggccgc catgcgcaac atcgacggcg ccatggagga gtcggctttg aacctgggtg
    28141 cgcgcggctg gcggctcttt cggcgcgtga tctttccgct cgccatgccg ggctacctgg
    28201 ccggcgcggc gctggtcttc gtcaaggtgt tcgacgacct gggcacgccc ctggtgatgg
    28261 gcgtgaccaa catgctggcg ccgcaggcct atctgcgcat cacctcggtg ggggtggatg
    28321 acccgctggg ctacgtgatc agcgtgatca tgatcgtgtt ctccatcctg gcgctgtggc
    28381 tggccgcgcg tgtgatgaag ggcaaggact acgccacgct gcaaaagggc ggcggcgcgc
    28441 tgcaaaagcg gcggttatcc aagtgggagg cgctgctggc ctatggctgg atagctctgg
    28501 tgctggccgt ggtgctggcg ccgcatgtgg gcattctgct gatgtcgctg gcccaggtgt
    28561 ggagcttctc ggtgctgccc gacagctaca cgctggcgca ctacgccacc gtgttcagcg
    28621 acgcgcgcgg catgatggcc aacaccctgc tgtactgcgt gctggccgcc gggctggacg
    28681 tggtcatagg cacggccatc gcctacctga ttctgcgcac gcggttgccg gcgcgccagt
    28741 ggctggactg gctggcctcg gccgcgctgg ccattcccgg tctggtgctg gcgattggct
    28801 atctgcggct gttcaagggc gtgacgctgc cgggcagcga tcagctcgtc gttcagacct
    28861 gggtgctgat catgctggcc tacgccgtgc ggcgcctgcc ttatgcgctg cgctcgtgca
    28921 tggcggcgtt gcagcaggtg catgtgtcgc tggaggaggc ggcgcaaagc gtgggcgcgt
    28981 cgcgcctgtc caccatacgg cgcgtgacgg tgccgctgat ggccggcggc attctggcgg
    29041 gtttcgtcac cagcttcatc acggcggcgg tggagctgtc ggccacgctg gtgctggtct
    29101 cgaccgaaag ccaggcgccg atgagcttag ggatatacct ctacatgcaa agcattgccg
    29161 ggcgcgggcc gggcgctgct ttgggcgtgc tggccgtgct cgtcgtgggc ctgggcacgt
    29221 atttctcgca ccgtttcgtg gagcgctcgc gcgccacagt cgcttcttct gccaaggcct
    29281 gaccatgaac caagcctccc ttgagtgccg ccatatcagc ctgtcctacg gcgccacgcc
    29341 ggtgctgcgc gatgtcaatt tgaagataga gccgggcgag ttttttgcgc tgctcggccc
    29401 ctcgggctcg ggcaagtcca cgctgttgcg cctgatcgcg ggcttcaacc agcaccagag
    29461 cggcgagctc ttgatcgacg gccaggacat ctccggcgtg ccgccgcatt tgcgcggcgt
    29521 gggcatggtg ttccagaact acgcgctgtg gccgcacatg acggtgtggg acaacgtggc
    29581 cttcggcctg gtggagcggc gcgagagccg cgacgccatc cgccgcaagg tgggcgcggc
    29641 actcgaattg gtcggcctgc aggactacgg ccagcgccgc cccggtcagc tctcgggcgg
    29701 ccagcagcag cgcgtggcgc tggcgcgcac ggtggtcatt gaacccaagc tgctgctact
    29761 cgacgagccc ctgtccaacc tggacaagca gctgcgcgtg cagatgcgcg aggaactgaa
    29821 gaacctgcag cgccagctgg gcctgaccac catcttcgtc acccacgacc aggaagaggc
    29881 catgaccacc gccgaccgca tggccgtgct ggaccagggc gtgctgcagc aggtgggcac
    29941 ggccgccggg ctgtatgacg agccgcacaa ccgctttgtg gcgggctttg tcggcaccgc
    30001 gaatctgctg gagggccagg tgagcgcctt ggacggcgac acgctggtgt tcgacgccca
    30061 gggcctgggc cgcctgacgc tgcccctgcc cgccgagccg cccgcgcccg gtgcggcggc
    30121 gctggcgttt cgcccgcacc agatccgcat cgccgccgag ggcgagccgg gtgatgcatc
    30181 gcgcgtctgg ctgcagggca gggtggaggc ggccgagttt ctgggcgagt ttgcgcgtta
    30241 ccgcgtgcgc gtgggcgagg tggcgctggt ggcagaccag ccgcattacg cgggcggcgc
    30301 catgttcccg cccggcgccc aggtgcggct ggggcttgcg cccacgcagc tgcgctatct
    30361 gcatcgctga gcctgcgccc tgcacgggcg ggggcgaatc cgtacactgc ggcgcttgtt
    30421 ttctgaagac gagcccatga acacgattcc ccttggtcaa agcgacttgc aggtcacccc
    30481 catctgcctg ggcaccatga cctttggcga gcaggtggat gagccgctgg cccacgccat
    30541 cctcgaccgc gccctggagc gcggcctgaa cttcatcgac acggccgaga tgtacgccgt
    30601 gcccgcgcgc gcggccacct acggcgccac cgaggccatc atcggccgct ggcttgcctg
    30661 ccgccccggc gcgcgcgcca aggtggtgct ggccagcaag gtggccggcc cctcgcgcgg
    30721 catgccctgg atacgcgagg gttccggcat gacggcggcc gacatccggg cctcctgcga
    30781 ggccagcctg cgccgcctgc agaccgattg catagacctc taccagatcc actggcccga
    30841 gcgccatgtg ccggtgttcg gccagctgta ctacgagccc gcccaggaga cctcgcaaac
    30901 ctccatccgc gagcagctgc aggcgctggc cgccctggtg caggccggca aggtgcgcca
    30961 catcggcctg tccaacgaga caccctacgg cgtgcatgaa ttcgtgcgcc tggccgagca
    31021 gcatggcctg ccgcgggtgg tgtcggtgca aaacccgtac ggcctggtga accgcagctg
    31081 ggacaacgcc atggacgaga gctgccaccg cctgggggtg tcgctgctgg cctattcgcc
    31141 gctcggcttt ggcctgctca ccggcaagta cgaccggcat gcgccgcatg aacccggcgc
    31201 gccccagcag gcgcgcatcg cccgctacga gtcggtgcgc aagcagcgct ggggccggcc
    31261 cgaggccctg gccgccgcgc gccgctacaa ccagctggcg cgcgaccatg gcctgacgcc
    31321 ggtgcagctg gcgctggcct tctgctacca gagctggcgc gtggccagca ccatcatcgg
    31381 cgtgaccacg ctggcacagc tggacgaaaa cctggacgcc tggggcacgg tgctgtcccc
    31441 cgagctgctg gccgccgtgg acgcgctgcg ctggcagctg cgcgaccccg ggcaatgatt
    31501 tgaataaaaa aacggctgta gcgcttatcc atcaagcgca agcagctatg atttttatat
    31561 atggccaaga aagacaagaa cgcccatgtg agcgaaaccc ccgccaccca gctgctgcgc
    31621 gcgcagggcg tggagttcac cgagcacccc tacgattacg tggagcatgg cggcagcgcc
    31681 gagtccgcgc gccagctggg cctggatgag cacatggtgg tcaagaccct ggtgatgcag
    31741 gaccaggacg ccaggccgct gatcgtgctc atgcatggcg accgcaaggt ctccaccaag
    31801 aacctggcgc gccagatcgg cgccaagcat gtacagccct gcaagcccga ggtggcgaat
    31861 cgccacagcg gctatctggt gggcggcacc tcgcccttcg gcacgcgcaa agagatgccg
    31921 gtgtttattg aggagagcat cctgcagctg ccgcgcatcg ccatcaacgg cgggcggcgc
    31981 ggctatctgt tgcagctcga cccacaggtg tgcgtgcggc tgctgcaggc gaaaaccgtg
    32041 cagtgcgcgc tggcagaata gggcgcttcg tttcctcctt ccttgtctgc cgtgagcatc
    32101 ctcctgtccc ttgccgccac cctggctgcc tatttgctcg gttccctgtc gttcgccgtc
    32161 atcgtcagcc gcgccatggg cctgtccgat ccgcgcagct atggcagcaa gaaccccggc
    32221 gctacgaatg tgctgcgctc tggct
//
LOCUS       DAOMTQ010000009        32132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_101_length_32132_cov_25.358668, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000009 DAOMTQ010000000
VERSION     DAOMTQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 32132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32132
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101_length_32132_cov_25.358668"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..909)
                     /gene="phoB"
                     /locus_tag="PK518_01955"
     CDS             complement(214..909)
                     /gene="phoB"
                     /locus_tag="PK518_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRM47703.1"
                     /translation="MKTSPRVLIVEDEPAIAELLSVNLRHNGFTPIWAEDGAAAQREI
                     DAQLPDLILLDWMLPGQSGLQLARKWRLDARSKAIPILMLTARGDEPDKVAGLDAGAD
                     DYITKPFSTQELLARIRAVLRRRAPEHGGESVSVGSLHLDPATHRVSYAGQPLKLGPT
                     EFKLLHHLMRHAERVHSRAQLLDKVWGDHVYIEERTVDVHVKRLREALGEAAVMVETV
                     RGAGYRVTAQGQA"
     gene            complement(906..1607)
                     /gene="phoU"
                     /locus_tag="PK518_01960"
     CDS             complement(906..1607)
                     /gene="phoU"
                     /locus_tag="PK518_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRM47704.1"
                     /translation="MTDKHLSTQFDSELNQVSARVMELGGLVESQIRQAIAALSQFSL
                     EAAERVEFIENRVNQMEVEIDHELITIIARRQPTARDLRLLMAFSKATANLERMGDEA
                     TRMARMVRSIIEHGAARSLPSSDLRMAADLASGLLRKALDAFARLDTRAALAILKEDD
                     LIDREFDGFVRKLITYMMEDARTISASLDLLFLAKAIERIGDHSKNIAELIIYLVEGK
                     DVRHQALEHIESALQ"
     gene            complement(1833..2618)
                     /gene="pstB"
                     /locus_tag="PK518_01965"
     CDS             complement(1833..2618)
                     /gene="pstB"
                     /locus_tag="PK518_01965"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRM47705.1"
                     /translation="MSTAPRPSQPPRAKLAVRDLNFYYGKFHALKGINLDIPENKVTA
                     FIGPSGCGKSTLLRTFNRMFELYPEQRAQGQILLDGEDLLTSRQDVALIRAKVGMVFQ
                     KPTPFPMSIYDNIAFGVKLFESLNATDMDERVEWALRKAALWTEVKDKLHQSGAGLSG
                     GQQQRLCIARGIAVLPEVLLLDEPCSALDPISTAKIEELIAELKNDYTVVIVTHNMQQ
                     AARCSDYTAYMYLGDLMEFGATEQMFFKPQRKETEDYITGRFG"
     gene            complement(2631..3512)
                     /gene="pstA"
                     /locus_tag="PK518_01970"
     CDS             complement(2631..3512)
                     /gene="pstA"
                     /locus_tag="PK518_01970"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRM47706.1"
                     /translation="MTTSQKLLSAATLAQTRQARFAARKRLNQVALALSLAAMAFGVF
                     WLVWILWETLRLGLSGLSLSLFTEMTPPPNEAGGLANALFGSGVMVLLATFVGTPIGI
                     LAGVYLAEYGRQGALAGCTRFVNDILLSAPSIVIGLFVYTVVVARFKSFSGWAGVLAL
                     ALIVIPVVIRTTENMLLLVPASLREAAYALGTPKWKVITLVTLRAARAGVITGVLLAV
                     ARIAGETAPLLFTALNNQFWNADLSKPMASLPVTIFKFAMSPYENWQQLAWAAVFLIT
                     VAVLGLNILARVLTRQK"
     gene            complement(3509..4549)
                     /gene="pstC"
                     /locus_tag="PK518_01975"
     CDS             complement(3509..4549)
                     /gene="pstC"
                     /locus_tag="PK518_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRM47707.1"
                     /translation="MQHKGHPVSTSLTSPAAPLAQPGSGQSRPPARRPWFSASWADRL
                     FAALARGAALLTLALLLGVLVSLVIGAWPAIAQYGLGFLTSTAWDPVQNEYGGLVMVY
                     GTLATSLIALLIAVPVSFGIALFLTELSPTWLKRPLGTAIELLAAVPSIVYGMWGLMV
                     FGPILATWVQQPLQALFKGVPVLGALVAGPPVGIGILAAGIILAIMIIPFIASVMRDV
                     FEVTPPLLKESAYGLGATTWEVMWKVVLPYTKSGVLGGVMLGLGRALGETMAVTFVIG
                     NMNQLNSLSVFEAANSITSALANEFAEAGEGLHQASLIYLGLVLFFITFVVLSASKLL
                     LSRLQKGEGARA"
     gene            complement(4604..5644)
                     /gene="pstS"
                     /locus_tag="PK518_01980"
     CDS             complement(4604..5644)
                     /gene="pstS"
                     /locus_tag="PK518_01980"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRM47708.1"
                     /translation="MKLCVLRVLVAGLVSAAALPQALAQSEATGAGASFPAPLYAKWA
                     ADYHKATGVKINYQSVGSGAGLRQIEAKTVDFGASDAPLKDEDLAKKGLVQFPTVIGG
                     VVPVVNIKGIAPGQLKLSGAVLGDIYLGKVTQWNDAAIAALNPGVALPDAAIAPVRRA
                     DGSGTSFIFTNYLSKVNAEWKAKVGEGTAVNWPIGAGGKGNEGVAAFVGRLPNSIGYV
                     EYAYVKQNKMNYVQLQNADGAFVTPDDDSFKAAAAGADWSKSFYQILTNQPGKAAWPL
                     TGATFILMHKAQDKPQQAAATLKFFEWAYKSGDKQAGELDYVPMPEAVKGQIAKSWGQ
                     IVDGAGKAVALK"
     gene            5852..7327
                     /gene="ppx"
                     /locus_tag="PK518_01985"
     CDS             5852..7327
                     /gene="ppx"
                     /locus_tag="PK518_01985"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRM47709.1"
                     /translation="MQDGTLLAAVDLGSNSFRLEIGRYEHGHIQRVEYLKETVRQGGG
                     LDEERNLSQAAMQRGWDCLARFAERLADFAPRQVRAVATQTLREAKNRDVFLERGNAI
                     LGHAIEVVSGPEEARLIYQGVSHLLPQSDERRLVVDIGGRSTELILGRQYQAHEVASF
                     RVGSVSWSKQFFADGQLTAAAFRQAETAAQAMLDEALAVYRPERWERAYGSSGTVGAV
                     AEILAACGHESGVIYRQGLDWLLQQLLRAGHTSRVELSGLKDDRRPVIGGGLSVLRAL
                     FDLLQIDRLHVAQGALRQGALYDLIDRSSPGTDKRSATVAGLAQRFAVDGAQAERVAR
                     AAGTLFAQATPQAGDDAARELGWAAQLHEIGQRIAHSGYHRHGAYILEHTDAAGFAQP
                     ELQRLGQLLRAHRGKLRKLDLDWDRPGLALQLACLRLAVALCHARRDPDLQGLRLQQQ
                     GQRLSLWLPAGWAERYPQSAHLLREEGLDWQRTPWVLEIFE"
     gene            complement(7569..8033)
                     /locus_tag="PK518_01990"
     CDS             complement(7569..8033)
                     /locus_tag="PK518_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM47710.1"
                     /translation="MMDLILWRHAEAHEGQEGEDDLLRRLTPRGEKQAARMAVWLDRN
                     LPEGLRVLASPARRAEQTAQALGRKFKLRAELLPQATPADLLELARWPQGRGAVLMVG
                     HQPVLGQTAAQLLDMPGADCAIRKGAVWWLRQRQRQGESQTVLLAAMSPDLL"
     gene            complement(8041..10212)
                     /gene="ppk1"
                     /locus_tag="PK518_01995"
     CDS             complement(8041..10212)
                     /gene="ppk1"
                     /locus_tag="PK518_01995"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM47711.1"
                     /translation="MLPLSPTKTEPALEASLSELGPIPPEAAPAALAPTPPAAPLLDR
                     DHSILAFNERVFDWARRPDVPLLERLRYLCIVSSNLDEFFEVRAAAHITAVTDGDTKG
                     AYTQASFEALMASAQGLVARQYQLYNEALAPALAAEGLRIVSHGQRTAEQRRWVQEYF
                     LREVRPLLVPVGLDPAHPFPMVANKSLNFIVRLKGSDAFGRSNEIAIVRVPRALPRVI
                     RIPARAAGKESIFVLLSSVIRAHLADLFPGRELGEFSQFRVTRHSELAVDEEDVSNLR
                     TALRQGLTQRNYGQSVRLEVSASCTRFLSDFLQAQFSLPAQAVFRVHGPVNLVRLGQL
                     VDLAERPSLLFPAWRAAWPQGLVQGHSILAQLRSNDVLMHHPFESFDAVLAFLREAVH
                     DPDVLVIKQTIYRTGADSELMELLREAVRRGKEVMAVVELKARFDEEANINWAEALEA
                     LGAQVVYGVVGLKTHAKMLLVTRREGKGLRRYGHLSTGNYNPRTARLYTDLGLLTSNE
                     DITADMDGVFSHLASQNRPPKLRQLMLAPFYLQQRMLEKIAQMGEAAQAGQYARIVVK
                     MNALTDEALIHALIRAGQQGAQIDLIVRGACMLPAQLPGLTDNIRVRSIIGRFLEHTR
                     VFYFAAGEQENLYLSSADWMNRNMLRRVELAWPVHDRALRQRIIDECLVAYLHDERNA
                     WTQRSDGSYAPPPRPGVGNGAQTALMSRYGGGALAKRKKRK"
     gene            complement(10389..11453)
                     /locus_tag="PK518_02000"
     CDS             complement(10389..11453)
                     /locus_tag="PK518_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM47712.1"
                     /translation="MTAPATASALPAQTDAVVIGAGPVGLFQVFQLGLQGLSAHVIDA
                     LPQPGGQCAELYGDKPIYDIPAIAACTGHDLTERLCAQIAPFAPAWHLGQQVDTVAAL
                     DQGGFLVVTARGTRLHARCVFIASGVGAFMPRQLKLPGLEAFEGRQLHYHLDDNTSLA
                     ARHIVIHGGGESAVRQAIACALAPEAIRAARITLLHRRDVFDASPKQLDELQALRAAG
                     RIQVAIGMPGRVVQQQGRLTALELTNPEGQAVELPLDMLLVRLGLVPRLGPIADWGLQ
                     LERKQLVVDGATFATSTPGIYAVGDAITYPGKRKLIVSGFHEATLAAFGAAEWLAGTR
                     LPLEYTTSSERLQTLLGRRG"
     gene            complement(11477..11800)
                     /locus_tag="PK518_02005"
     CDS             complement(11477..11800)
                     /locus_tag="PK518_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRM47713.1"
                     /translation="MTHVVVENCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCA
                     VCVPECPANAIFAEEDLPSDQLAFIKLNAELTPKFKSITKRKAALPDADEWNGQPDKL
                     KDLER"
     gene            complement(11906..13276)
                     /locus_tag="PK518_02010"
     CDS             complement(11906..13276)
                     /locus_tag="PK518_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM47714.1"
                     /translation="MTKTIETIAASAVSTGENSINDHISALRFITCGSVDDGKSTLIG
                     RLLVDSRAVLQDHLAGVTRSGEVDLAQLTDGLSSEREQGITIDVAYRYFATEERKFII
                     GDAPGHEQYTRNMVTAASQADAAVVLVDATKLDWQNPQLELLVQTRRHSLLVHLLRVH
                     SLVFAVNKLDAVADPSQAFTNIRAALLRFAQDAGIQVAATVPVSALKGWNVVNPHPGW
                     CGYQGHSLLQVLELLPHTPADTQLPLAFPVQWVEKSASSSADTRQGRRVFWGRVAAGS
                     VAPGAQVSLFPSGQKAVVAQVLDHARRPTGVDAGSSAGIVLDREVDVSRGDWILAAPS
                     AAAPADDDFDAPVEHSAWPGRREISATVAWMDDEPLVVGRVYWALHGHRWVKAKVRRV
                     VHCLNIHTLAKEGAHQLEPNAIGHVELVLQEPLPVAAFEQARVLGALILVDQASHKTA
                     GAVLAH"
     gene            complement(13276..14208)
                     /gene="cysD"
                     /locus_tag="PK518_02015"
     CDS             complement(13276..14208)
                     /gene="cysD"
                     /locus_tag="PK518_02015"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRM47715.1"
                     /translation="MNARVDPQVLSQLSNRHLDALEEETIFILREVAAAFERPALLFS
                     GGKDSLVMLRCAEKAFGAGNIPYPLLMIDTGHNFPEVTDFRDFRAKELGAELIVRSVE
                     DSMARGTVRLSHPGESRNVHQSVTLLEAIEEFRFDALIGGARRDEEKARAKERIFSHR
                     DSFGQWQPKAQRPELWTLFNTRIAPGEHFRVFPISNWTELDVWQYIAREQIGLPQLYY
                     AHQRQVVERRGLLVPVTPLTPAKDGEQVETRTVRFRTVGDITCTCPVESPAASAAEIV
                     IETLAAEVSERGATRMDDKTSEASMEKRKKDGYF"
     gene            complement(14273..14905)
                     /locus_tag="PK518_02020"
     CDS             complement(14273..14905)
                     /locus_tag="PK518_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HRM47716.1"
                     /translation="MSAVTPAELARVNAELGRDAAGLTRWALGLGKNAIVTTNFRPFE
                     AVILHLVTRVQPDMPVVWMDNGYNTEATYRYADEVTRRLGLNLKTYLPLRTRAHREAV
                     EGPTPALDDPRHESFTQEVKLEPFARALREQAPQVWFTALRASDSAVRAQMEPVSINP
                     DGLIKVAPLLHWSSKDLYQYCEIHGLPNNFDYVDPTKGEEQRECGLHLAH"
     gene            complement(14902..15297)
                     /locus_tag="PK518_02025"
     CDS             complement(14902..15297)
                     /locus_tag="PK518_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HRM47717.1"
                     /translation="MKIISANAVPTGENAENVLQIANDADLAALHASGALQGVERIEL
                     QFPKFTDGRAYSQALLLRRRYQFAGDIRATGDVLIDQLVHMHRSGFSSAVLAGGVDAS
                     AAERQFARFAAFYQGDVNEPRPLFAREAA"
     gene            complement(15325..17115)
                     /locus_tag="PK518_02030"
     CDS             complement(15325..17115)
                     /locus_tag="PK518_02030"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRM47718.1"
                     /translation="MYQYTDFDKQFVKLRAQQFRDQLERWQKGELSDEQMLPLRLQNG
                     WYIQRYAPMARIAVPYGEISSLQLRTLGHIAREYDRPSPELLASAQATQDALEASQPG
                     ATLAAPALQYGYGHFTTRTNCQFNWIPITRAADVMDLLASVSMHGIQTSGNDIRNITC
                     DAWEGIAQDSIVDCRPFAEITRQWSSLHPEFSYLPRKFKIAFNGAEEDRAAIGWYDIG
                     LQALKNDAGEVGFRVLVGGGMGRTPIVGSVVREFLPWAELLNYIEAIVRVYNGYGRRD
                     NKWKARIKILVKSEGQAYIDAVEKEYQAIVEQDGAPHRITQAEFDRVASHFVIPELKP
                     ANLPSQVNPQGQLYQRWLAQNVRGHRLAGMKTVTLSFKRSGFAPGDADAATLEALAEL
                     AEQFSASEARLTHEQNLLLPWVFESDLPALYEAARKLGLAQANIGLLTDMIACPGGDF
                     CSLANARSLHIAHAVTERYQDLDELLDIGPIDLHMSGCINSCGHHHSGHIGILGVDKD
                     GKEWYQVTLGGADGTRLSGPSIGGKVVGPAFTAAEVPDVIEAVLQTYRKLRQPGEYFI
                     DALRRIGHDPFKQAANGARHPKLAEEALPA"
     gene            complement(17284..19155)
                     /gene="mnmC"
                     /locus_tag="PK518_02035"
     CDS             complement(17284..19155)
                     /gene="mnmC"
                     /locus_tag="PK518_02035"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HRM47719.1"
                     /translation="MSETIAWLPDGTPYSTRFDDRYHGEQGLAQARDVFLHGCGLPAA
                     WAGAQQWRILETGFGFGLNFLATWAAWRADPARPRLLHFVSLEAWPVSADDLLRAAQR
                     HPELADLAQELHAQYWGLLPGIHRLWLDDGHVLLTLCIGDAQALLRQQLWEVDAVYLD
                     GFNPQRNPAIWSPETLKAMARHCRRGTRLATWTVARSVRDALTQCGFQVEMVPGLPPK
                     RDNLRACYDPHWQPRSSRQAPEFTQPARCLVLGAGLAGATAAASLARRGWQVTLLDAA
                     RAPAAGASGLPVGLFAPHLSPDDNLFARVSRAGVRAMLQQSGALLQAGVDWNPAGVLE
                     RRPAGELGLPAAWADGPGTDWSQAAGADHLAAAGLPLDTSACWHTRAGWVCPARLIAR
                     LLAQPGIALRPNSPVARLQHTSPSLWQVLDGTGAVLAEAELVVVAAGPASNALLVQLA
                     GPRLPLQSVRGQMSWGLQPEPAPGLPPFAVNGGGALMAHVPCEGALAWHMGATFERDV
                     ERLPMTIAEQADAHMHNWQRLQALVPTAAATLRPAFAPGGDLRAWSGVRCTSQDRMPI
                     VGPVDAAALPGLWISTAMGARGLTRAVLCAELLAARLMGEPLPVETKLARAMGTERL"
     gene            complement(19165..19830)
                     /locus_tag="PK518_02040"
     CDS             complement(19165..19830)
                     /locus_tag="PK518_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRM47720.1"
                     /translation="MLPSASLLESFQTSQISFMLVIYLLAITAEAMSGALAAGRRNMD
                     VFGVTVIAFVTALGGGTIRDMVLGHFPIGWTQHPEYVYLVIAAGLLTTVVARFMHHLK
                     QLFLWLDALGLIAFSLIGCSVALELDYPTVVVVMSGMITGISGGILRDVLCNQVPVVF
                     RRELYASVSMAVCLLFLALRAWDVEHNLSTAVCFVGGLALRLAAIRWHWRLPVFSYQQ
                     RWE"
     gene            complement(20018..20290)
                     /locus_tag="PK518_02045"
     CDS             complement(20018..20290)
                     /locus_tag="PK518_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HRM47721.1"
                     /translation="MARTVHCIKLDKEAQGLDFPPYPGELGKRIFENVSKQAWGDWLK
                     HQTMLVNENRLNLADARARQYLARQMENHFFGSGADAAAGYVPPKA"
     gene            complement(20542..21444)
                     /locus_tag="PK518_02050"
     CDS             complement(20542..21444)
                     /locus_tag="PK518_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRM47722.1"
                     /translation="MTPSLSILAPLSVALFCAAPASAASAQRATGDSALATSLREQAI
                     AYEHAEGVTRDTARAAALYCESARLGDMVAQYNLGWMYAHGRGVARDDETAAFFFHAA
                     AEQGLDVAARMLQVVGGSTGKMPGCMRPAPQATTKAAPPGVDYQAIAPRKIVEMVHKM
                     APQYQVEPQLALAIIAAESNFNPQALSPKNAQGLMQLIPETSERFQVKNPYDPAQNIR
                     GGLSYLRWLLAYFEGDVALVAAAYNAGEGKVERYRGVPPYLETRAYVQRILKAVGTAI
                     HPFDRTVTQPSPALERIRLVLRPQ"
     assembly_gap    21574..21673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21832..22920
                     /locus_tag="PK518_02055"
     CDS             21832..22920
                     /locus_tag="PK518_02055"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRM47723.1"
                     /translation="MQHHRAPRIVAHTVVEGLEHPWAVAFLPDGRYLISERPGRLRVA
                     TPEGRLLAPITGLPAIASGGQGGLLDVALDSDFTSNRRLYFCFSEPGSGADQGKNSTA
                     LARARLSQDDRRLEDLQVIFSQRPKIASRLHFGCRIVERRVGGRGDGTLFLTLGERFS
                     EKDQAQNLQSHLGKIVRVGKDGRVPPDNPFVGRDSVWPEIWSLGHRNLQGAALAPDGA
                     LWTHEHGPQGGDEVNRPEPGRNYGWPVITYGVNYGGGSIGAGITHHAGMEQPLHYWVP
                     SIAPSGMAFITSDRYGPAWQGSLVVGALKSRMLERLSVRGGQVTATEPLLPQLAQRVR
                     DVRQGPDGWLYLLTDEDPGQLLRLQLAP"
     gene            23038..23223
                     /locus_tag="PK518_02060"
     CDS             23038..23223
                     /locus_tag="PK518_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47724.1"
                     /translation="MPVFKPGTRVKRAGSWETVSHVTLRRNDLAVYLVGSTEAVDPME
                     LELEPTVFTTERVPGAY"
     gene            complement(23234..24085)
                     /gene="asd"
                     /locus_tag="PK518_02065"
     CDS             complement(23234..24085)
                     /gene="asd"
                     /locus_tag="PK518_02065"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014409.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HRM47725.1"
                     /translation="MSDRLAVLPQYLLPKQLLTTLAGRLASAHAGSLTTAAIRRFVAR
                     YRVDMSEAAEPDITGYASFNDFFTRALRPGLRPLADAAVVCPVDGAVSQLGPIERDQI
                     FQAKGHHYSTTALLGGNAQLAAQFEDGHFATIYLSPRDYHRIHMPVDGRLLSMEHVPG
                     ALFSVNPTTARGVPGLFARNERVVCIFDTPLGPMALVLVGATIVGSMATVWHGQVNPP
                     RTGQPRRWDYSDQQITLRQGDEMGRFLLGSTVVLLFARSAIQFEPGWAAERPVRLGQA
                     MAQHSAG"
     gene            24229..25383
                     /locus_tag="PK518_02070"
     CDS             24229..25383
                     /locus_tag="PK518_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HRM47726.1"
                     /translation="MFLPPSQNPGNEPGTSRALVWLRLFKPARVRINQRERWRMVLGM
                     VMCIAVVALFAHSLGMASSPPWMVASLGASAVLIIALPSSPLAQPWPVLAGTVLSYLV
                     GVACAALVPSLSLASGLAVGLSVAVMLALRCLHPPGGGMALFAVLHPHDSTAAGALPV
                     AFNVLALVLAGMAFNHLTGRSYPHAQARPPSTRKESGAFTSADLDAALSHYNQVLDVS
                     RDDLEGLLHLAGKAAFQRTLGELRCRQIMSAPVHVVVADTPLKDAWALMRKQAIKALP
                     VVDEQQAVIGIVTMADFMRLANLEVHEGMGQRLRTLILGRPKRPEQVQGLMSAPVLQV
                     LAEQHVMDLVPLFSDAGHHHIPVVNEEQQLVGIITQSDLVKTLAAAVTQT"
     gene            25628..25894
                     /gene="infA"
                     /locus_tag="PK518_02075"
     CDS             25628..25894
                     /gene="infA"
                     /locus_tag="PK518_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRM47727.1"
                     /translation="MAKEELIEMQGSVTEVLPDSRFRVTLDNGHQLIAYTGGKMRKHH
                     IRILAGDKVSLEMSPYDLTKGRITFRHLAGRGPGPGTGAPPARR"
     gene            25958..26034
                     /locus_tag="PK518_02080"
     tRNA            25958..26034
                     /locus_tag="PK518_02080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25992..25994,aa:Pro,seq:tgg)
     gene            26145..27359
                     /locus_tag="PK518_02085"
     CDS             26145..27359
                     /locus_tag="PK518_02085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM47728.1"
                     /translation="MLGVFVCHTFRPKQKGAVLQQDNPPSSGLIRRAMDALLTTDRDQ
                     RIRLSMSVMAALLMLCCIAALQLVAAAGLADAGHVHWFSVVCSLGLIAVYWLIRSGYS
                     QRWEDPALTFLQIVSSVTCTAVAYVIAGSARGITLPILAVILIFGVFGLSPRQMLFVL
                     FYGIAVFGVALSVAQWGLAHGSQPLALTAAYMVMIVVMLASCTFINLRVLASRKRKLE
                     LAQAAANERERAIRDELTGLHNRRFMLEIMHLEGARVQRNHQPLLIAQLDLDHFKHIN
                     DTHGHDAGDQVLAAFARTVAGYIRSTDILARWGGEEFVLLMVNTAADNGVALLERVRA
                     AVQESVLVMATGASIRVTVSIGVAQLQTGHETPIGLLRRADEALYAAKAQGRNRVVLA
                     DQTLLETTVATG"
     gene            27346..28122
                     /locus_tag="PK518_02090"
     CDS             27346..28122
                     /locus_tag="PK518_02090"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HRM47729.1"
                     /translation="MPRVDPGPRGGSAARHDLVDALRGLAMVWMTVFHFCFDLSHFGY
                     WQQDFLGDPFWTVQRTLIVSLFLLCAGMGQAVAWHRRLAWPRFWRRWGQIAACALLVT
                     VGSYLMFPRSFIYFGVLHGMAVMLLIARLTAGWGRWLWPMGLLAVALPWLAGWALEGP
                     LAGLAETFNGRALNWLGWVTHKPFTEDYVPLFPWLGVMWWGMAGGQWLLARRPHWLAW
                     AMPGTGVALAVLGRYSLSYYMLHQPVMMGALMLVGWFMQP"
     gene            complement(28173..28958)
                     /locus_tag="PK518_02095"
     CDS             complement(28173..28958)
                     /locus_tag="PK518_02095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM47730.1"
                     /translation="MKKSLLSGLVAAAVLGTMALPVAAQNLAIVNGKAVPKERVETLK
                     QQVERSGRPISPEMENQIKEEVIAREIFMQEAQKRGLEATSDYKAQMELARQTILIRE
                     LFVDFQKTNPVTDAEIQAEYDKFAAANAGKEYKASHILVEKEDEAKAIIAALKKGGKF
                     EEIAKKQSKDPGSGARGGDLDWANPSSYVPEFTEALVKLEKGKLTQTPVKSQFGWHII
                     RLDDVREAQLPKIDEVKPQIAQQLQQQKLAKFQEDLRAKAKVE"
     gene            complement(29027..29302)
                     /locus_tag="PK518_02100"
     CDS             complement(29027..29302)
                     /locus_tag="PK518_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRM47731.1"
                     /translation="MTATPIAITAEAMEARLREVLAPTQLQVLDESWQHEGHPGANGT
                     GFGTHFRVRIASPRFAGQRKVAQHRLVYDALQIFIDHGAHAIAIETL"
     gene            complement(29299..29856)
                     /locus_tag="PK518_02105"
     CDS             complement(29299..29856)
                     /locus_tag="PK518_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HRM47732.1"
                     /translation="MKLLIDFFPIILFFVAFKAWGIYVATGVAIVATIAQIAYIHFKH
                     GKVEPMQWLSLGVIVLFGGATLLAQSETFIKWKPTVLYWLMGGALLVGQLLFRKNFIQ
                     SLMGAQIELPAPVWLKLNWAWTGFFSAMGVLNLWVAYNFDTDTWVNFKLFGGMGLMLA
                     FVVAQALYLSRHMKEDGTTAKDAQP"
     gene            complement(29853..30266)
                     /gene="msrB"
                     /locus_tag="PK518_02110"
     CDS             complement(29853..30266)
                     /gene="msrB"
                     /locus_tag="PK518_02110"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRM47733.1"
                     /translation="MTYAIQKTDAEWQALLRDKGAEPAAFQVTRHAATERPFTGKYER
                     HWADGSYHCICCGARLFDSGTKFDAGCGWPSFSLALPGAIQEIEDRSHGMRRVETVCA
                     QCGAHLGHVFPDGPAPTGLRYCMNSASLAFEPPAP"
     gene            complement(30263..31747)
                     /locus_tag="PK518_02115"
     CDS             complement(30263..31747)
                     /locus_tag="PK518_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HRM47734.1"
                     /translation="MPASTVCPPPATTPAQPLLAGWHDGFAALGPAFHTELRPTPLPA
                     PYWVGTSADVGTLLGLPVGWPQRDDALQVFTGNVLLPGSRPLASVYSGHQFGVWAGQL
                     GDGRAILLGETAGGQEVQLKGCGRTPYSRMGDGRAVLRSSIREFLCSEAMYALGIPTT
                     RALCVTGSPAPVQREELESAAVVTRVAPSFIRFGHFEHFAARGQEAELRQLADYVIER
                     YYPQCSGTSNPYAQLLQAVSERTAALLAQWQTVGFCHGVMNTDNMSILGLTIDYGPFQ
                     FLDAFDPNHICNHSDHLGRYAFNRQPGVAYWNLLCLAKALLPLIRDVEQAQQALDGYE
                     PVFIEQFMAHMRAKLGLRRAREGDETLVDSLQQLLAKNRVDYTIFWRRLSRAVADGKD
                     EPVRDLFADRTAFDAWSLQYKELLALDDKAMAADLMLKTNPCFVLRNHLAEQAIRAAK
                     LGDLSVLQTLQRLLARPFDEHPGFEAYADFPPDWASSLSISCSS"
     gene            31905..>32132
                     /locus_tag="PK518_02120"
     CDS             31905..>32132
                     /locus_tag="PK518_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229102.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRM47735.1"
                     /translation="MLGLMQNQPLLISLLITFAERNHGDGEIVSRRVEGDIHRCTYRD
                     LARRARQLANTLDGMGLQFSDRVATLAWNGYR"
BASE COUNT         5152 a        10142 c        11054 g         5684 t
ORIGIN      
        1 agccaggcca gcacccgcag gccgcgccag gccacccagc cccagcacag ccaggaggcc
       61 agcgccacgc ccgccgccag cccccaggcg ccggccagcc acagccccag ggccgcgccc
      121 agcagctggg cgatgagcag catcgctatg cgccagaaca tgatcaaaaa atgcctctat
      181 ggcaataggg tagtgcgcag gcagctatca tttttaagcc tgtccctggg ccgtgacgcg
      241 gtagcccgcg ccgcgcaccg tctccaccat caccgcggcc tcgcccagcg cctcgcgcag
      301 gcgcttgaca tgcacgtcca cggtgcgctc ctcgatgtag acatggtcgc cccagacctt
      361 gtccagcagc tgcgcgcggc tgtgtacacg ctcggcgtgg cgcatcaggt ggtgcagcag
      421 cttgaactcg gtggggccga gcttgagcgg ctggccggcg tagctgacgc ggtgcgtggc
      481 cgggtcgaga tgcaggctgc cgacgctgac actttcgccg ccatgctcgg gcgcgcgccg
      541 gcgcagcacg gcgcggatgc gcgccagcag ctcctgcgtg gaaaagggct tggtgatgta
      601 gtcatcggcg ccggcgtcca ggcccgcgac cttgtcgggc tcgtcgccgc gcgcggtcag
      661 catcaggatg gggatggcct tgctgcgcgc atccaggcgc cacttgcgcg ccagctgcag
      721 gccgctctgg cccggcagca tccagtcgag caggatcagg tcgggcagct gcgcgtcgat
      781 ttcgcgctgc gcggccgcgc cgtcctcggc ccagatgggc gtaaagccgt tgtggcgcag
      841 gttcacggac agcagttcgg cgatcgccgg ttcgtcttcg acgatcagaa cacgggggga
      901 ggttttcatt gaagtgccga ctcgatgtgt tccaacgcct ggtggcgcac gtccttgcct
      961 tcgaccaggt agatgatgag ttcggcaatg ttcttggaat ggtcgccgat gcgctcgatg
     1021 gccttggcca ggaacagcag gtccaggctg gccgagatgg tgcgcgcatc ttccatcatg
     1081 taggtgatga gcttgcgcac gaagccgtcg aactcgcggt cgatcaggtc gtcttccttg
     1141 aggatggcca gcgccgcgcg ggtgtccagg cgcgcaaacg cgtccagcgc cttgcgcagc
     1201 aggcccgagg ccaggtcggc ggccatgcgc aggtcgctgc tgggcagcga gcgcgccgcg
     1261 ccgtgctcga tgatggagcg caccatgcgc gccatgcgcg tggcctcgtc gcccatgcgc
     1321 tccagattcg ccgtcgcctt ggagaaggcc atcaacaggc gcaggtcgcg ggcggtgggc
     1381 tggcgccggg cgatgatggt gatgagctcg tggtcgatct cgacctccat ctggttcacg
     1441 cggttctcga tgaactcgac gcgctcggcc gcctccaggc tgaactgcga cagggcggcg
     1501 atggcctggc gaatctgcga ctcgaccagg ccgccgagct ccatcacgcg ggcggagacc
     1561 tggttgagtt cgctgtcgaa ttgggtggag aggtgtttgt ctgtcatggt gtctctagtt
     1621 tttctccctc tcccgctggc gggagagggt gggggtgagg gcgattcggt ggcaggcgcc
     1681 gtgcctgctc taccccctca cccctgccct ctcccccaag ggggcgaggg agagaaaaaa
     1741 tcagccgaag cggccggtga tgtaatggtt acggccgctg cgcttaactt cgctgcgcga
     1801 agtgagcctc cgccgttgca tcactgctcg gttcatccga acctcccagt gatgtaatcc
     1861 tcggtttcct tgcgctgggg cttgaagaac atttgctcgg tggcgccgaa ctccatcagg
     1921 tcgcccaggt acatataggc cgtgtagtcg ctgcagcgcg ccgcctgctg catgttgtgc
     1981 gtgacgatga cgacggtgta gtcgttcttc agctcggcaa tcagttcctc gatttttgcg
     2041 gtggagatgg ggtccagcgc cgagcagggt tcatccaaga gcagcacctc gggcagcacc
     2101 gcgatgccgc gcgcaatgca caggcgctgc tgctgcccgc ccgagaggcc agcgccgctc
     2161 tggtgcagct tgtccttgac ctcggtccac agcgcggcct tgcgcagcgc ccattccacg
     2221 cgctcgtcca tgtcggtggc gttcaggctc tcgaacagtt tcacgccaaa ggcgatgttg
     2281 tcgtagatcg acatcggaaa cggcgtgggc ttctggaaca ccatgccgac cttggcacgg
     2341 atcagcgcca cgtcctggcg actggtcagc aggtcttcgc cgtccagcag gatctgcccc
     2401 tgggcgcgct gctcggggta gagctcgaac atgcggttga aggtgcgcaa gagcgtcgat
     2461 ttgccgcagc ccgaggggcc gatgaaggcc gtgaccttgt tctcgggaat gtcgaggttg
     2521 atgcccttga gcgcgtggaa cttgccgtag tagaagttca ggtcgcgcac ggcgagcttg
     2581 gcacgcggcg gctgggaggg gcggggagcg gtggacatgt tggttgttcc ttatttttgg
     2641 cgcgtcagca cgcgcgccag gatgttcagg cccagcaccg ccacggtgat gaggaagacg
     2701 gcggcccagg cgagctgttg ccagttctcg taggggctca tggcaaattt gaaaatggtg
     2761 accggcaggc tggccatggg cttactcagg tcagcgttcc agaactggtt gttgagcgcc
     2821 gtgaacaaaa gcggcgccgt ctcgcccgca atgcgcgcca cggccagcag cacgccggta
     2881 atcacacccg cgcgcgcggc gcgcagcgtg accagcgtaa tcaccttcca cttcggcgtg
     2941 cccagggcgt aggccgcttc gcgcaggctg gcaggcacca gcagcagcat gttctcggtc
     3001 gtgcggatga ccacggggat gacgatcagc gccagcgcca gcaccccggc ccagcccgaa
     3061 aagctcttga aacgcgccac caccacggtg tagacgaaca ggccgatgac gatggagggc
     3121 gccgacagca ggatgtcgtt gacgaaacgc gtgcagcccg caagcgcacc ctggcgcccg
     3181 tattcggcca ggtagacccc cgccagaatg cctatgggcg tgccgacaaa ggtggccagc
     3241 agcaccatca cgcccgagcc gaacagcgcg ttggccaggc cgccggcctc gttgggcggc
     3301 ggcgtcatct cggtgaagag cgagagcgac aggcccgaca ggcccaggcg cagcgtctcc
     3361 cacaaaatcc acaccagcca gaacacgcca aaggccatgg cggcgagcga gagcgccagt
     3421 gccacctggt tcaggcgctt gcgtgcggca aagcgggcct ggcgggtctg cgccagggtg
     3481 gcggcagaaa ggagcttttg tgacgtcgtc atgcgcgcgc tccctcgccc ttttgcaggc
     3541 ggctcagaag cagcttggac gccgacagca cgacgaaggt gatgaagaac agcaccagcc
     3601 ccaggtagat gagcgacgcc tggtgcaggc cctcgccggc ctcggcgaat tcgttggcca
     3661 gggccgaggt gatgctgttg gcggcctcga acaccgagag ggagttgagc tggttcatgt
     3721 tgccgatgac aaaggtcacc gccatggttt cgcctaaagc gcggcccagg cccagcatca
     3781 cgccgccgag cacgccggac ttggtgtagg gcagaaccac cttccacatc acctcccagg
     3841 tggtggcgcc caggccgtag gccgactcct tgagcagtgg cggagtcact tcaaacacat
     3901 cgcgcatcac cgaggcgatg aagggaatga tcatgatggc caggatgatg ccggccgcca
     3961 gaatgccaat gcccaccggc gggccagcca ccagcgcgcc cagcaccggc acgcccttga
     4021 agagagcctg cagcggctgc tgcacccagg tggcgaggat ggggccgaac accatcagcc
     4081 cccacatgcc gtagacgatg gagggcacgg cggccagcag ctcgatggcc gtgcccagcg
     4141 ggcgcttgag ccaggtgggc gagagttcgg tgaggaacag cgcaatgcca aagctcaccg
     4201 gcaccgcgat gagcagggcg atgagcgagg tggccagcgt gccgtagacc atgaccaggc
     4261 cgccgtactc gttctgtacc gggtcccagg ccgtgctggt caggaaaccc aggccgtact
     4321 gcgcgatggc gggccaggca ccaatgacca gcgacaccag cacgcccagc agcagcgcca
     4381 gggttagaag ggcggcgcca cgcgccagcg cggcgaacag ccggtcggcc cagctggcgg
     4441 aaaaccaggg gcggcgcgcc ggcgggcgcg actggccgct gcccggctgc gccagcggcg
     4501 cggcgggaga agtcaaggag gtggacacgg ggtggccttt gtgttgcatg gtgcggcgtt
     4561 ttttctccca cccccagagg gggaaggagt gaaaagccgc agattatttc agtgccaccg
     4621 ccttgcccgc gccgtccacg atctggcccc aggatttggc gatctggcct ttgacggcct
     4681 cgggcatggg cacgtagtcc agttcgccgg cctgcttgtc gccgctcttg taggcccatt
     4741 caaagaactt gagtgtggct gcggcctgct gcggcttgtc ctgcgccttg tgcatcagga
     4801 tgaaggtggc gccggtcagc ggccaggcgg ccttgccggg ttggttggtc aggatctggt
     4861 agaagctctt gctccagtcg gcaccggcgg ccgcggcctt gaagctgtcg tcgtcaggcg
     4921 tcacaaaggc gccatcggcg ttttgcagct gcacgtagtt cattttgttt tgcttgacat
     4981 aggcgtactc cacgtagccg atggagttgg gcaggcggcc gacgaaggcg gccacgccct
     5041 cgttgccctt gccgccggcg ccgatgggcc agttcaccgc cgtgccttcg cccaccttgg
     5101 ccttccactc ggcgttcacc ttggagaggt agttggtgaa gatgaagctg gtgcccgagc
     5161 cgtcggcgcg gcgcaccggg gcgatggctg catccggcag cgccacgccg gggttcagcg
     5221 cggcgatggc ggcgtcgttc cactgggtga ccttgcccag gtagatgtcg cccagcacgg
     5281 cgccgctcaa tttcagctgg cccggggcaa tgcccttgat gttgaccacc ggcaccacgc
     5341 caccgatcac ggtggggaac tgcaccaggc cctttttggc caggtcttcg tccttgagcg
     5401 gcgcgtccga ggcgccaaag tccaccgtct tggcctcgat ctggcgcagg cccgcgcccg
     5461 agccgacgga ctggtagttg atcttcacgc ccgtggcctt gtggtagtcg gcggcccatt
     5521 tggcgtacag cggcgccgga aagctcgcgc ccgcgccggt ggcctcgctc tgggccaggg
     5581 cttggggcag ggcggcggca gacaccaggc ccgccaccag aacacgtaac acacacagct
     5641 tcatgaaaaa cctttgaagg ttgttgaaag ggatgcagcg caatgtaggc atgctttgtg
     5701 acagcgctgt gacatatggg cggcccggaa gctggtggca agccgccgac tgcgcgctgt
     5761 cacggcgctg tcatgcaggc ttcgtaggct gcgcggttga ccattgaccc cggtgctatg
     5821 ctgcgccgct ttgtttgcct ccccccctcg tatgcaagac ggaacattgc tcgccgccgt
     5881 ggacctgggg tccaacagct ttcgcctgga aattggccgc tacgagcatg gccatatcca
     5941 gcgcgtcgaa tacctcaagg aaaccgtgcg ccagggcggc ggcctggacg aggaacgcaa
     6001 tctgagccag gccgccatgc agcgcggctg ggactgcctg gcacgctttg ccgagcgcct
     6061 ggccgacttt gccccccggc aggtgcgcgc cgtggccacg caaaccctgc gcgaggcgaa
     6121 gaaccgggac gtttttttgg agcgcggcaa cgccatcctg ggccatgcca tcgaggtcgt
     6181 ctcgggcccc gaagaggcgc gcctgatcta ccagggtgtg tcgcacctgc tgccccagtc
     6241 cgacgagcgc cgcctggtgg tggacattgg cgggcgctcc accgagctca tcctgggccg
     6301 gcagtaccag gcgcacgagg tggcctcgtt tcgcgtcggc agcgtgtcct ggtccaaaca
     6361 gttctttgcc gatggccagc tgacggccgc tgccttccgc caggcggaga ccgccgccca
     6421 ggccatgctc gacgaggccc tggccgtgta ccggcccgag cgctgggagc gggcctacgg
     6481 atcgtcgggc acggtgggcg cggtggccga gatcctggcc gcctgcgggc atgagagcgg
     6541 cgtgatctac cgccagggcc tggactggct gctgcagcag ctgctgcgcg cgggccacac
     6601 cagccgcgtg gagctgtccg gcctcaagga cgaccgccgc cccgtcatcg gcggcggcct
     6661 gagcgtgttg cgcgcgctgt tcgacctgct gcagatcgac cgcctgcatg tcgcccaggg
     6721 ggccctgcgc cagggtgcgc tgtacgacct gatcgacaga tcttcacccg gcactgataa
     6781 gcgcagcgcc accgtggccg gcctggcaca gcgctttgct gtcgatggcg cccaggccga
     6841 gcgcgtggcg cgggcggccg gcaccctgtt tgcccaggcc acgccccagg ccggcgacga
     6901 cgccgcgcgc gagctgggct gggccgcgca gctgcatgag atcggccagc gcatcgccca
     6961 cagcggctac caccgccacg gcgcctacat cctggagcac accgacgccg ccggctttgc
     7021 ccagcccgag ctgcagcgcc tgggccagct gctgcgcgcc caccggggca agctgcgcaa
     7081 gctcgacctc gactgggatc gccccgggct ggcgctgcaa ctggcctgcc tgcgcctggc
     7141 cgtggccctg tgccacgcgc ggcgcgaccc cgacctgcag ggcctgcgcc tgcagcagca
     7201 gggccagcgc ctgagcctgt ggctgcccgc cggctgggcc gagcgttacc cgcagtcggc
     7261 ccacctgctg cgcgaggaag gcctggactg gcagcgcacg ccctgggtgc tggaaatttt
     7321 tgagtgattt tggcctgatt aggcatatac ctcagactgt gccgcgagcc ctcaccccaa
     7381 ccctctccca gagggagagg gagtaaagcc ggcgcaaccg gtcgccctcg cccctttggg
     7441 gagagggagg ggtgaggggc ctctgcgata tgtgcatagt caggggtttt ggccgttatc
     7501 gcttgtatat gaagcgcccg cagctacaaa ttcaatagtg aatggccctg cggctggccc
     7561 gcccgctgtt acaaaagatc gggcgacatg gccgccagca acacggtctg gctttcgccc
     7621 tggcgctggc gctggcgtag ccaccacacc gctcccttgc gtatcgcgca atccgcgccc
     7681 ggcatgtcca gcagctgggc cgccgtttgc cccagcacgg gctggtggcc caccatcagt
     7741 acggcgccgc gcccctgggg ccagcgggcg agctccagca gatccgcggg cgtggcctgg
     7801 ggcagcagtt cggcgcgcag cttgaatttg cgccccagcg cctgggcggt ctgctcggcg
     7861 cgccgcgccg ggctggccag tacgcgcagg ccctcgggca ggttgcggtc gagccagacg
     7921 gccatgcgcg cggcctgctt ctcgccgcgc ggcgtcaggc ggcgcagcag gtcgtcttcg
     7981 ccctcctggc cctcatgggc ctcggcgtgg cgccacagaa tcaggtccat catcctggaa
     8041 tcacttgcgt ttcttgcgtt tggccaacgc gccgccgccg tagcggctca tgagggcggt
     8101 ttgcgcgccg ttgccgacac cggggcgcgg tggcggggcg tagctgccgt cgctccgctg
     8161 ggtccaggcg ttgcgctcgt cgtgcaggta ggccaccagg cattcgtcga tgatgcgctg
     8221 gcgcagcgcc ctgtcgtgca ccggccaggc cagctccacg cggcgcagca tgttgcggtt
     8281 catccagtcg gcgctggaga ggtagaggtt ctcctgctcg cccgcggcga agtagaagac
     8341 ccgcgtgtgc tcgagaaagc gcccgatgat ggagcgcacg cggatgttgt cggtcagccc
     8401 cggcagctgc gccggcagca tgcaggcgcc gcgcacgatg aggtcgatct gcgccccctg
     8461 ctggccggcg cgtatcaggg cgtgtatcag cgcctcatcg gtgagcgcat tcatcttgac
     8521 gacgatgcgc gcgtactgcc cggcctgcgc cgcctcgccc atctgcgcaa tcttctccag
     8581 catgcgctgc tgcaggtaga agggcgcgag catgagctgg cgcagtttgg gcgggcggtt
     8641 ctggctcgcc aggtggctga agacgccgtc catgtcggcg gtgatgtcct cgttgctggt
     8701 gagcaggccc aggtcggtgt acaggcgtgc cgtgcgcggg ttgtagttgc cggtcgacag
     8761 gtgcccgtag cggcgcaggc ccttgccctc gcggcgcgtc accagcagca tcttggcgtg
     8821 ggtcttgagg cccaccacgc cgtagaccac ctgcgcgccc agggcctcca gtgcctcggc
     8881 ccagttgatg ttggcctcct cgtcaaagcg cgccttcagc tccaccacgg ccatcacttc
     8941 cttgccgcgg cgcacggcct cgcgcagcag ctccatcagt tcggagtcgg ccccggtgcg
     9001 gtagatggtc tgcttgatga ccagcacatc ggggtcgtgc acggcctcgc gcaggaaggc
     9061 gagcacggca tcaaagctct cgaacgggtg gtgcatcagc acatcattgc tgcgcaattg
     9121 cgccaggatg gaatggccct gcaccagccc ttgcggccag gcggcgcgcc aggcgggaaa
     9181 cagcagcgac gggcgttcgg ccagatcgac gagctggccc aggcgcacca ggttcaccgg
     9241 cccatgcacg cgaaacaccg cctgcgccgg caggctgaac tgggcctgca gaaagtccga
     9301 cagaaagcgt gtgcagctcg ccgacacctc caggcgcacg gactggccgt agttgcgctg
     9361 tgtcaggccc tggcgcaggg cggtgcgcag gttgctcacg tcttcctcgt ccaccgccag
     9421 ctcggaatgg cgcgtgacgc gaaactgcga gaactcgccc agctcgcgcc cgggaaacag
     9481 atcggccagg tgcgcgcgga tgacgctgga gagcagtacg aagatcgatt ccttgccggc
     9541 ggcgcgcgcc ggaatgcgga tcacgcgcgg cagggcgcgc ggcacgcgca cgatggcaat
     9601 ctcgttgctg cggccaaagg cgtccgagcc cttcaggcgc acgatgaagt tgagcgactt
     9661 gttggccacc atggggaagg ggtgggccgg gtccagtccc acgggcacca gcagcgggcg
     9721 cacctcacgc aggaagtact cctgcaccca gcgccgctgc tccgccgtgc gctggccgtg
     9781 cgagacgatg cgcagtccct cggccgccag ggccggggcc agggcctcgt tgtagagctg
     9841 gtactggcgc gccaccaggc cctgggccga ggccatcagg gcctcgaagc tggcctgggt
     9901 ataggcgccc ttggtgtcgc cgtcggtgac tgccgtgata tgcgcggccg cgcgcacctc
     9961 gaagaactcg tccaggttgg acgagacgat gcacaggtag cgcaggcgct ccagcagggg
    10021 gacatcgggg cgccgggccc agtcgaacac gcgttcgttg aaggccagga tgctgtggtc
    10081 gcggtccagc agcggcgccg ccgggggcgt tggcgccaat gccgcggggg cggcttcggg
    10141 cgggatgggg ccgagttcgc tcaggctcgc ctcaagggcg ggctccgtct tggtggggga
    10201 gaggggcaac atggatacgg cgacagtgag cgacccgcgc agtatcagaa ggctttgtga
    10261 cagagtgatg acgcatctgt gacgcggcgg cggcggtggc gggtgcctgc ccgtggcatg
    10321 cggggcatgc ccgccaatgc ggcctttttc tccatgccgc agggcgtgcc cgagcacgcc
    10381 ccaggctttc agccgcgcct gcccagaagg gtttgcagcc gttcgctgct ggtggtgtat
    10441 tccagtggca gcctggtgcc cgccagccac tcggcggcgc caaaggcggc cagggtggct
    10501 tcgtgaaagc cgctcacgat gagtttgcgt ttgccggggt aggtgatggc atcgcccacg
    10561 gcgtagatgc cgggggtgct ggtggcaaag gtggcgccgt ccaccaccag ctgcttgcgt
    10621 tcgagctgca ggccccagtc ggcaatcggc cccaggcgtg gcaccaggcc caggcgcacg
    10681 agcagcatgt ccagcggcaa ctccacggcc tggccctcgg ggttggtgag ctccagggcc
    10741 gtcaggcgtc cctgctgctg cacgacgcgg ccgggcatgc cgatggccac ctggatgcgg
    10801 ccggcggcgc gcagcgcctg cagctcgtca agctgctttg gcgacgcgtc gaagacatcg
    10861 cggcggtgca gcagcgtgat gcgcgcggcg cggatggcct cgggggccag ggcgcaggca
    10921 atggcctgcc gcaccgcgct ctcgccgccg ccgtggatga cgatatgccg ggcggccagg
    10981 ctggtgttgt cgtccaggtg atagtgcagc tggcggcctt caaaggcctc cagcccgggt
    11041 agcttgagct ggcgcggcat gaaggcgccg acgccgctgg cgatgaagac gcaacgcgca
    11101 tgcagccgcg tgccacgcgc cgtgaccacg agaaaaccgc cctggtccag ggcggccacg
    11161 gtatcgacct gctggcccag atgccaggcg ggggcaaagg gcgcgatctg cgcgcacagg
    11221 cgctctgtca ggtcgtgccc ggtgcaggcg gcgatggccg ggatgtcgta gatgggtttg
    11281 tcgccataca gctcggcgca ttgaccgccg ggctggggca gggcgtcgat gacatgggcc
    11341 gagagaccct gcaggcccag ctgaaagacc tggaacagcc ccaccggacc ggcgccgatc
    11401 accacggcat cggtctgcgc cggcaatgcg ctggcggtgg cgggcgctgt catcaacggc
    11461 ccgggcgggt gggcgctcag cgctccagat ccttgagctt gtccggctgg ccgttccatt
    11521 cgtcggcgtc gggcagggcg gccttgcgtt tggtgatgct cttgaacttg ggcgtcagct
    11581 cggcattgag cttgatgaag gccaactggt cgctgggcag atcttcctcg gcaaagatcg
    11641 cgttggccgg gcattcgggc acgcagacgg cgcagtcaat gcactcgtcg gggtcgatca
    11701 cgagcatgtt cgggccttcg cggaagcagt ccacggggca tacatccacg caatcggtgt
    11761 atttacactt gatgcagttc tcgacaacga cgtgggtcat gggggttcag ttgctctagc
    11821 tattgggtat aacccgcgat tttaggccca tggtcagccg ccggggcagc cgaccatggg
    11881 ccaggtgcgg aaaacttgat gcgggtcaat gcgccagtac ggcgcctgcg gtcttgtggc
    11941 tggcctggtc caccaggatc aatgcgccca gtacccgcgc ctgctcaaac gccgccacgg
    12001 gcaggggctc ctgcaacacc agctcgacat ggccgatggc gttgggttcc agttgatgcg
    12061 cgccttcctt ggccagggtg tggatgttca ggcagtgcac cacccggcgc accttggcct
    12121 tgacccagcg gtggccatgc agtgcccaat agacgcggcc aaccaccagg ggctcgtcgt
    12181 ccatccaggc gacggtggcg ctgatctcac gccggcccgg ccaggcgctg tgttcgacgg
    12241 gggcgtcaaa gtcatcgtcg gccggtgctg ccgcgctggg cgcagcgagg atccagtcgc
    12301 cacgcgagac atccacctcc cgatcgagga cgatgccggc gctgctgccg gcgtccaccc
    12361 cggtcggccg gcgagcatgg tccagcacct gggccacgac cgccttctgg ccgctgggga
    12421 acaggctgac ctgcgccccc ggcgccaccg agcccgcagc cacgcggccc cagaacacgc
    12481 gccggccctg gcgcgtgtcg gccgacgaag aagcggactt ttccacccat tgcaccggaa
    12541 aggccagcgg cagttgcgtg tccgccggtg tgtgtggcag cagttccagg acttgcagca
    12601 ggctgtggcc ctggtagccg caccagcccg gatgggggtt gaccacgttc cagcccttga
    12661 gcgccgagac gggcacggtg gcggcgacct ggatgcccgc gtcctgtgca aagcgcagca
    12721 gcgccgcgcg gatgttggtg aaggcctgcg acgggtcggc cacggcgtcg agtttgttca
    12781 ccgcaaacac cagcgaatgc acgcgcagca ggtgcaccag caggctgtgg cggcgcgttt
    12841 gcaccagcag ttcgagctgc gggttctgcc agtcgagctt ggtggcatcg accagcacca
    12901 cggccgcgtc ggcctgcgag gctgccgtaa ccatgttgcg cgtgtactgc tcgtggccgg
    12961 gggcatcgcc gatgatgaat ttccgctcct cggtggcgaa gtagcgatac gccacgtcga
    13021 tggtgatgcc ctgctcgcgc tcggacgaca ggccgtcggt gagctgcgcc agatccacct
    13081 cgcccgagcg cgtcacgccc gccaggtgat cctgcagcac ggcgcggcta tcgaccaaga
    13141 ggcgcccgat cagcgtgctc ttgccgtcat cgacgctgcc gcaggtgatg aagcgcagag
    13201 cggaaatatg atcgtttatg gagttttcgc ctgtagatac ggcgctagca gctattgttt
    13261 cgatagtttt tgtcattaga agtacccgtc tttcttgcgc ttttccatgc tcgcttcgct
    13321 ggtcttgtcg tccatgcgcg tggcgccgcg ttcgctcacc tcggcggcca gggtttcgat
    13381 cacgatctcg gccgcgctgg cggcggggct ttccaccggg caggtgcagg tgatgtcgcc
    13441 cacggtgcga aagcgcacgg tgcgcgtctc cacctgctcg ccgtccttgg ccggggtcag
    13501 cggcgtcacc ggtaccaaca ggcctcggcg ctcgaccacc tggcgctggt gcgcgtagta
    13561 cagctggggc aggccaatct gctcgcgcgc gatgtactgc cacacgtcca gctcggtcca
    13621 gttgctgatg gggaagacgc ggaaatgctc gcccggggcg atgcgggtgt tgaacagcgt
    13681 ccacagctcg ggtcgctgcg ccttgggctg ccattggcca aagctgtcgc ggtgggaaaa
    13741 gatgcgctcc ttggcgcgcg ccttttcctc gtcgcggcgc gcgccgccga tcagtgcgtc
    13801 gaagcgaaac tcttcgatgg cctccaggag cgtgaccgac tggtgcacgt tgcggctctc
    13861 gcccgggtgc gaaaggcgca ccgtgccgcg tgccatggag tcttcgacgc tgcgcacgat
    13921 gagttcggct cccaattcct tggcgcgaaa gtcgcgaaag tcggtcacct cggggaagtt
    13981 gtgcccggtg tcgatcatca agagcgggta gggtatgttg ccggcgccaa aggctttttc
    14041 ggcgcagcgc agcatgacca gcgaatcctt gccgcccgag aacagcaggg ccgggcgctc
    14101 aaaagcggcg gcgacttcgc gcaggatgaa gatggtttct tcttcgagcg catcgaggtg
    14161 gcggttgctg agctggctga gcacttgggg gtcaacacgg gcgttcatgg gagtatccat
    14221 tcaaattcgg ttgtagcact gataggtaaa gcgctgatag ctatcatttt tatcagtgcg
    14281 ccaggtgcag gccgcattcg cgctgctcct cgcccttggt cgggtccacg tagtcaaagt
    14341 tgttcggcag gccgtggatt tcgcagtact ggtacaggtc cttggacgac cagtgcagga
    14401 gcggcgccac cttgatcaga ccgtcggggt tgatgctgac gggctccatc tgcgcacgca
    14461 cggcgctgtc gctggcgcgc agcgccgtga accagacctg cggtgcctgc tcgcgcaggg
    14521 cgcgggcgaa gggctccagc tttacttcct gcgtgaagga ctcgtggcgc gggtcatcca
    14581 gcgcgggcgt ggggccttcg acggcctcgc ggtgcgcgcg cgtgcgcagc ggcaggtagg
    14641 ttttcaggtt caagcccagc cggcgcgtga cctcgtcggc gtagcggtag gtggcctcgg
    14701 tgttgtagcc gttgtccatc cagaccacgg gcatgtcggg ctgcacccgg gtgaccaggt
    14761 gcaggatgac ggcttcaaac ggacggaaat tggtcgtcac gatggcgttt ttgcccaggc
    14821 ccagcgccca gcgcgtcagg ccggcggcgt cgcggcccag ttcggcgttg acgcgcgcaa
    14881 gttctgcagg ggtgacggca ctcatgcggc ctcccgggca aagagcgggc gcggctcgtt
    14941 cacatcgccc tggtagaagg cagcaaaacg ggcgaactgg cgctcggccg cgctggcatc
    15001 cacgccgcct gccagcacgg cgctgctaaa gccactgcgg tgcatgtgca ccagctggtc
    15061 tatcagcaca tcgcccgtgg cgcggatgtc gcccgcaaat tggtagcgcc ggcgcagcag
    15121 cagggcctgg ctgtaggcgc gcccgtcggt gaacttcgga aactgcagct cgatgcgttc
    15181 tacgccctgc agcgcaccgc tggcgtgcag cgcggccagg tcggcgtcgt tagcgatttg
    15241 caagacattt tcggcgtttt cgcccgtagg tactgcgtta gcagatatta ttttcatagc
    15301 agtaattctc gtgggaacgg ggcttcaagc cggcagggct tcttcggcca gcttggggtg
    15361 gcgcgcgcca ttggccgctt gcttgaacgg gtcgtggccg atgcggcgca gggcgtcgat
    15421 gaagtattcg cccggctggc gcagcttgcg gtaggtttgc agcacggcct cgatcacgtc
    15481 gggtacctcg gcggcggtga acgccgggcc gacgaccttg ccgccaatcg aggggccgga
    15541 cagccgcgtg ccgtcggcac cgcccagcgt cacctggtac cactccttgc cgtccttatc
    15601 gacgcccaga atgccgatat ggccgctgtg atggtggccg cagctgttga tgcagccgct
    15661 catgtgcagg tcgatggggc cgatgtccaa gagctcgtcc aggtcctggt agcgctcggt
    15721 gaccgcgtgg gcgatgtgca gcgagcgcgc attggccagc gagcaaaaat ccccgcccgg
    15781 gcaggcgatc atgtcggtca agaggccgat attggcctgc gccaggccga gcttgcgcgc
    15841 cgcctcgtac aaggcgggca ggtcagactc gaacacccag ggtagcagca ggttttgttc
    15901 gtgcgtcagg cgtgcttccg atgcgctgaa ctgctcggcc agctcggcca gggcctccag
    15961 ggttgcggcg tcggcgtcgc ccggggcaaa gccgctgcgc ttgaacgaca gcgtcacggt
    16021 cttcatgccc gccagccggt ggccgcgcac gttctgcgcc agccagcgct ggtagagctg
    16081 gccctgcggg ttcacttggc tgggcagatt ggcgggcttc aattcaggga tgacgaagtg
    16141 gctggccacg cggtcaaatt cggcctgggt gatgcggtgc ggtgcgccgt cctgctcgac
    16201 gatggcctgg tattccttct ccaccgcatc gatataggcc tggccctcgc tcttgaccag
    16261 aatcttgatg cgcgccttcc acttgttgtc gcgccggccg tagccgttgt acacgcgcac
    16321 gatggcctcg atgtagttga gcagttcggc ccagggcagg aattcgcgca ccaccgagcc
    16381 gacgatgggc gtgcgcccca tgccaccgcc caccagcacg cgaaaaccca cctcgcccgc
    16441 gtcgtttttg agcgcctgca ggccgatgtc ataccagcca atggcggcgc ggtcttcctc
    16501 ggcaccgttg aaggcgatct tgaacttgcg cggcaggtag ctgaactcgg ggtgcagcga
    16561 gctccactgg cgcgtgatct cggcaaacgg gcggcaatcg acgatgctgt cctgggcaat
    16621 gccctcccag gcgtcgcagg tgatgttgcg gatgtcgttg ccgctggtct ggatgccgtg
    16681 catggagacg ctggccagca ggtccatcac gtcggccgcg cgggtgatgg gaatccagtt
    16741 gaactggcag ttggtgcgcg tggtgaagtg gccatagccg tattgcagcg ctggtgcggc
    16801 cagggtggcg ccgggctggc tggcctccag ggcgtcttgc gtggcctggg cgctggccag
    16861 cagctcaggg ctggggcggt cgtactcgcg cgcaatatgg cccagggtgc gcagctgcag
    16921 gctgctgatc tcgccgtagg gcacggcaat gcgcgccatg ggggcgtagc gctggatgta
    16981 ccagccgttt tgcaggcgca gcggcagcat ctgctcgtcg ctgagttcgc ctttttgcca
    17041 gcgctctaac tggtcgcgga actgctgggc gcgcagcttg acgaattgct tgtcgaagtc
    17101 ggtgtattgg tacatggtgg gccgtggggc gattcaatcg ttgcaccacc gggccctttt
    17161 ggcgcgccca gcagcgggga tgggcgggac tgtaaaaggc agtcttataa aaacaaacga
    17221 atatttagaa cttcatatat gcataaatgt gcatatgaaa cggaatgtcg aggttcgctc
    17281 gggctatagc cgctccgtgc ccatggcgcg cgccagcttg gtctcgaccg gcaatggttc
    17341 gcccatcagg cgcgccgcca gcagttcggc gcagagcacg gcacgtgtca atccgcgcgc
    17401 gcccatggcg gtgctgatcc acaggccggg cagggcggct gcatccaccg ggccgacgat
    17461 gggcatgcgg tcttgcgagg tgcagcgcac gcccgaccag gcgcgcaggt cgccgccggg
    17521 cgcaaaggcc ggacgcagcg tggccgcggc ggtgggcacc agggcctgca ggcgctgcca
    17581 gttatgcatg tgggcgtcgg cttgttcggc gatggtcatc ggcaggcgtt ccacatcgcg
    17641 ctcaaaggtt gcgcccatat gccacgccag agccccttcg cagggcacat gggccatgag
    17701 cgcgccgccg ccgttgacgg caaagggtgg caggccgggt gccggctcgg gttgtaggcc
    17761 ccagctcatc tggccgcgca ccgactgcag cggcagccgt gggccggcca gttgcaccag
    17821 cagggcgttg ctcgccgggc cagcggccac gaccaccagc tcggcctcgg ccagcacggc
    17881 gcctgtgccg tccagcacct gccaaaggct gggggaggtg tgctgcagcc gtgccacggg
    17941 gctgttcggg cgcaaggcga tgccgggctg cgccagcagc cgggcgatca aacgcgccgg
    18001 gcacacccag ccggcgcggg tgtgccagca agcgcttgtg tccaggggca ggccggcggc
    18061 ggccaggtga tcggcgcctg ccgcctgact ccagtccgtg cccgggccat cggcccaggc
    18121 ggcgggcaag cccagttcgc cggcggggcg gcgctcaagc accccggccg ggttccagtc
    18181 cacgcccgcc tgcaagagcg caccagactg ctgcagcatg gcacgcacgc cggcgcgtga
    18241 gacgcgggca aacaggttgt cgtcgggcga caagtgcggc gcaaacagcc ccaccggcag
    18301 gccggaggcg ccagcagcag gggccctggc agcatcgagc agcgtgacct gccagccacg
    18361 gcgcgcgagg ctggcggcag ctgtggcccc ggccaggccc gcgcccagca ccaggcagcg
    18421 tgccggctgg gtgaattcgg gcgcctggcg gctgctgcgc ggctgccagt gcgggtcgta
    18481 gcaggcgcgc aggttgtcgc gcttgggagg caggccgggc accatctcca cctggaagcc
    18541 acattgcgtg agcgcatcgc gcacgctgcg cgccactgtc cacgtcgcca ggcgcgtgcc
    18601 acggcggcag tggcgcgcca tggccttgag ggtttccggg ctccagatcg ctgggttgcg
    18661 ctgcgggttg aagccgtcga ggtacaccgc gtccacttcc cacagctgct ggcgtagcag
    18721 cgcctgggcg tcgccaatgc atagcgtcag cagcacatgc ccatcgtcaa gccatagccg
    18781 gtggatgccg ggcagcaggc cccagtactg ggcgtgcagc tcctgggcga ggtcggccaa
    18841 ctctgggtgg cgctgggccg cgcgcagcag gtcgtcagcc gacaccggcc aggcctcgag
    18901 cgaaacaaaa tgcagcaggc gcgggcgggc cgggtcggcg cgccaggcgg cccaggtggc
    18961 caggaagttc aggccaaagc caaagccggt ctccaaaatg cgccactgct gcgccccggc
    19021 ccacgccgct ggcagaccgc agccgtgcag aaacacgtcg cgcgcctggg ccaagccctg
    19081 ttcgccgtgg tagcgatcgt caaaacgggt gctgtaaggg gtgccgtcag gcagccaggc
    19141 gatggtttcg gacatggatg tgtgtcattc ccagcgctgc tggtaggaga acaccggcag
    19201 ccgccaatgc cagcgaatgg ccgccagccg caacgccagg ccaccgacaa agcacacggc
    19261 ggtgctcagg ttgtgttcca catcccaggc gcgcagcgcc aggaacagca ggcatacggc
    19321 catggagacg ctggcgtaga gctcgcgccg gaacaccacc ggcacctggt tgcacagcac
    19381 gtcacgcagg atgccgccgc tgatgccggt gatcatgccg ctcatgacca ccaccaccgt
    19441 ggggtagtcc agctccagcg ccacgctgca gccgatcagc gagaacgcga tcaacccaag
    19501 tgcgtccagc cacagaaaca gctgcttgag gtggtgcatg aagcgcgcca ccacggtggt
    19561 cagcaggccg gcggcaatca ccaggtacac gtattcgggg tgctgcgtcc agccgatcgg
    19621 gaagtggccc agcaccatgt cgcgtatggt gccgccgccc agggcagtga cgaaggcgat
    19681 caccgtcacg ccaaagacat ccatgttgcg ccgcccggcc gccagtgcac cggacatggc
    19741 ctcggccgtg atggccagca ggtagatcac cagcatgaag ctgatctgcg aggtttggaa
    19801 cgattccagc agggaggccg atggaagcac ggtgatcttt gggagttcgg ggtctgcaca
    19861 aggtctgcgc cgctgccgaa gaagtggttt tggcgaaacc acttctggca aggcttcact
    19921 gtgttacgcc ttgggcggga catagcccgc cgcagcgtct gcgccgctgc cgaagaagtg
    19981 gttttggcga aaccacttct ggcaaggctt cactgtgtta cgccttgggc gggacatagc
    20041 ccgccgcagc gtctgcgccg ctgccgaaga agtggttttc catctggcgg gccaggtatt
    20101 ggcgggcgcg ggcgtcggcc aggttgaggc ggttttcatt caccagcatg gtctggtgct
    20161 tgagccagtc gccccaggcc tgtttgctca cgttttcgaa gatgcgcttg cccagttcac
    20221 cggggtaggg cgggaagtcg aggccctggg cttccttgtc gagtttgatg caatggacgg
    20281 tacgtgccat ggagtgcttt ctgagtgaat ggggtggaaa taggacactg aacgctactg
    20341 cgttgagttg gaaggccggc gttcagcgtt ggggataggt ggccttgatc agcagcacca
    20401 aggcaacgct ggccttcgtg aaaagttgcc gtgactttat caagtttcgt gttgccgacg
    20461 ccctgagtga ggtcatggca ctggtggttt tgtcaccgtg gtcagcgcag tatcctgcgc
    20521 cagcggccgg cgccactgtc gctattgggg ccggagtacc agccgaatgc gttcgagtgc
    20581 cggcgaaggc tgcgtgacgg tgcgatcgaa cgggtgtatg gcagtgccga cggccttcag
    20641 gatgcgctgc acataggctc gcgtttccag gtaagggggc acgccgcggt agcgctccac
    20701 cttgccttca ccggcgttgt aggcggctgc caccagcgcc acatcaccct cgaaataggc
    20761 cagcagccag cgcaggtatg acaatccgcc ccggatgttc tgcgccgggt cataggggtt
    20821 cttgacctga aagcgctcgc tggtctctgg aatcagctgc atcaatccct gcgcattctt
    20881 gggtgatagc gcctgggggt tgaagttcga ctcggcggca atgatggcca gagccagctg
    20941 cggctcgacc tggtactgag gcgccatctt gtgcaccatc tcgacgatct tgcgcggtgc
    21001 aattgcctgg tagtccacgc caggtggagc tgccttggtt gttgcctgtg gtgccgggcg
    21061 catgcacccg ggcatcttgc cggtgctgcc gccaacgacc tgcaacatgc gtgcggcgac
    21121 gtccagcccc tgctcggctg ccgcgtgaaa gaagaaggct gccgtttcgt cgtcgcgtgc
    21181 cacgccccgc ccatgggcat acatccatcc aaggttgtac tgggccacca tatcgccaag
    21241 ccgcgcgctc tcgcagtaca gcgctgcggc ccgggccgta tcgcgtgtca ctccctcggc
    21301 gtgttcgtag gcgatggcct gctcgcgcag gctggtggcc agggcgctgt cgcccgtagc
    21361 gcgctgcgcc gatgccgcgc ttgcgggcgc ggcgcagaac aaggccaccg acagcggggc
    21421 aagaatcgag aggctcggcg tcatggcttt gagtatgcgc gtctttgtgg cgtacgtcat
    21481 gtcggcgcgg atcatgccgg ccgcggcatt tgccagaatg gggccatagc ccgggagtca
    21541 cgtcatgcgc cgacctgcca gccaccgccc caannnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnntggggcc atagcccggg agtcacgtca tgcgccgacc tgccagccac
    21721 cgccccaacc ttgctcatct tgttcgctcg cccctgcgcg gggtggctgc attcgcgctg
    21781 gccagtaccg cgctggccag taccgcgctg gcccagtccg cagcgccagg cgtgcagcac
    21841 catcgcgccc cgcgcatcgt ggcgcacacc gtggttgaag ggctggagca cccctgggcc
    21901 gtggcctttt tgcccgatgg ccgctatctc atcagcgaac gcccgggccg gctgcgcgtg
    21961 gccacgcccg aggggcgcct gctggcgccc atcaccgggc tgcccgccat tgccagcggc
    22021 ggccagggcg ggctgctcga cgtggcgctg gacagcgact tcacaagcaa ccgcaggctg
    22081 tacttctgct tttccgagcc tggcagtggt gccgaccagg gtaagaacag cacggcgctg
    22141 gcgcgggcgc ggctgtctca ggacgaccgg cggctggaag acctgcaggt catcttttcg
    22201 cagcgcccca agatcgccag ccgcctgcat ttcggctgcc gcatcgtcga gcgccgggtt
    22261 ggcggtcggg gcgatggcac attgtttctg acgctgggcg agcgcttcag tgagaaagac
    22321 caggcgcaga acctgcaaag ccacctgggc aagatcgtgc gcgtgggcaa ggacgggcgc
    22381 gtgccgcccg acaacccctt cgtgggccgc gactctgtct ggcccgagat ctggagcctg
    22441 ggccaccgca acctgcaggg cgccgcgctg gcgcccgacg gcgcgctgtg gacgcacgag
    22501 cacggccccc agggtggcga cgaggtcaac cgccccgagc cagggaggaa ctacggctgg
    22561 cccgtgatca cctatggcgt gaactacggt ggcggctcga ttggtgcagg catcacccac
    22621 cacgccggca tggagcagcc gctgcattac tgggtgccat cgattgcgcc ctcgggcatg
    22681 gccttcatca ccagtgatcg ctatggcccg gcgtggcagg gcagcctggt ggtgggcgcg
    22741 ctcaagagcc gcatgcttga acgcctgagc gtgcggggcg gccaggtcac cgccaccgaa
    22801 cccttgctgc cgcaactggc acaacgtgtg cgggacgtgc gccagggccc ggacggctgg
    22861 ctgtacctgc tgaccgacga ggatcccggg caactgttgc ggctgcaact cgcaccgtga
    22921 tcgttgcggc ccgatcaacc ctttcgacta agatggcacg ccatggagat catccaaacc
    22981 cccattgaag gcgccgacca accagaggta atttggggtt ggtccaggca atttcgcatg
    23041 cccgttttca agccgggtac gcgtgtcaag cgtgcaggca gttgggaaac cgtgagccat
    23101 gtaacgctgc ggcgcaatga tctggccgtg tatctggtgg gcagcaccga ggccgttgat
    23161 cccatggagc tggagttgga gccgacggtg tttaccaccg agcgcgtacc cggggcgtat
    23221 tgacgtttct cagtcagcca gcgctgtgct gcgccatggc ctgacccagg cgcacggggc
    23281 gctccgcagc ccagcctggc tcaaactgta tggcgctgcg cgcaaacagc agcaccacgg
    23341 tggagccaag caaaaaacga cccatttcat cgccctggcg cagcgtgatt tgctgatccg
    23401 aatagtccca gcggcgcggc tggccggtgc gtggtggatt cacctgacca tgccacacgg
    23461 tggccatgct gccgacaatc gtggccccca ccagcaccag ggccatgggg ccgagcgggg
    23521 tgtcaaaaat gcacaccacg cgctcgttgc gcgcaaacag accgggcacg ccgcgcgccg
    23581 ttgtcgggtt gaccgaaaac agcgcaccag gcacatgctc catgctgagc aaacggccat
    23641 cgaccggcat atggatgcgg tgatagtcgc gcgggctcaa atagatggtg gcgaaatggc
    23701 catcctcaaa ttgcgccgcc agttgcgcat tgccgcccag cagggcggtg gtggagtagt
    23761 ggtggccctt ggcctgaaaa atctggtcac gttctatcgg gcccagctga ctcaccgccc
    23821 catccaccgg gcacaccacg gctgcatcgg ccagggggcg caggccgggg cgcagtgcgc
    23881 gcgtgaagaa atcgttgaag ctcgcgtagc cggtgatgtc gggctcggca gcctcactca
    23941 tgtcaacgcg gtagcgcgcc acgaatcggc ggattgcggc cgtggtcagg ctgccggcat
    24001 gtgccgaggc caaccggccg gcaagggtgg tgagcagttg tttgggcagc agatactgcg
    24061 gaagcacggc gaggcggtca gacacgtagc ggcaatcttt ggaaaaaata gccgggccat
    24121 tctatagagt gatcgggcct gatctgacca gcccaataac catgccggca cattgccgac
    24181 aagccgcatt gataatgcgc ggccccgcgt ggtgcggctt gcctttccat gtttttgcca
    24241 ccatcccaga accccggtaa cgagcctggc accagccgtg cgctggtgtg gttgcgcctg
    24301 ttcaaacctg cgcgcgtgcg catcaaccag cgcgagcgct ggcgcatggt gttgggcatg
    24361 gtcatgtgca tcgccgtggt tgcgctgttc gcacattcgt tgggcatggc ctcgtctcca
    24421 ccgtggatgg tggcttcgct gggggcgagt gcggtgctga tcattgcctt gccctccagt
    24481 ccattggcgc aaccctggcc ggtgctcgcg ggaactgtcc tgtcctatct cgtgggcgtg
    24541 gcctgtgcgg cgctcgtacc cagcctgtcc ttggccagcg gcctggccgt cggcctgtcc
    24601 gtggcggtga tgctggccct gcgctgcctg cacccgccgg gcggaggcat ggcgttgttt
    24661 gccgtgctgc atccacacga cagcacggct gcaggggcat tgccggtggc cttcaacgtg
    24721 ctggccttgg tgctggcggg catggccttc aatcacctga cgggccgcag ctacccccat
    24781 gcgcaggcca ggccgccgtc aacgcgcaag gaatctggcg ccttcacgtc ggctgacctc
    24841 gatgccgcac tgtcacatta caaccaggtg ctggacgtca gtcgcgacga tctggagggc
    24901 ctgctgcatc tggccggcaa ggcagcgttt cagcgcaccc tgggcgagct gcgctgccgg
    24961 cagatcatgt cggcgcccgt gcatgtggtg gtggccgata cgccgctgaa ggatgcctgg
    25021 gcattgatgc gcaaacaagc catcaaggcc ctgccggtgg tggacgagca gcaggcggtc
    25081 atcggcatcg taaccatggc cgacttcatg cggctggcca atctggaggt gcatgagggc
    25141 atggggcagc gcctgcgtac gctcatcctg gggcggccca aacggcccga gcaggtgcag
    25201 ggcttgatgt cggcgccggt actgcaggtg ctggccgagc agcatgtgat ggatctggtg
    25261 ccgctgtttt ctgatgcggg gcaccatcac atcccggtgg tgaacgagga gcagcagctg
    25321 gttggcatca tcacccagtc cgatctcgtg aaaacattgg ctgcagctgt aacgcaaacc
    25381 taggtgcaag ccagcgcatc gcagtacaat gctcggcttc gtacatagcg ccgcgcttgg
    25441 ccattgaagc tgccgttacc cggtctgttg gcatccagcc atcgggtttg cgactcccct
    25501 gcctgtacca gcactgtgtc agcactttgc gccgggttcc tgaagggcct gcgatcgctg
    25561 gctatggttg cgcaggactt tccatgagcg ccgccacaga catttgttat ctagagattg
    25621 accacatatg gccaaagaag agctgatcga gatgcagggc agcgtcaccg aagtcctgcc
    25681 agactcgcgt tttcgcgtga ccctggacaa cgggcaccag ttgatcgcct acaccggcgg
    25741 caagatgcgc aagcaccata tccgcattct tgccggcgac aaggtgtcgc tggagatgtc
    25801 gccctacgac ctgaccaagg gccgtatcac cttccgccac ctggccggac gcggccctgg
    25861 gccgggcacg ggtgctccgc cggccaggcg ttgatggcga caaaaatcgc gccgttcgcc
    25921 tgtgaaccat ggtttggcag gttagaatgc cagctgtcgg agcgtagcgc agcctggtag
    25981 cgcatctgct ttgggagcag agggtcgcga gttcgaatcc cgccgctccg accaataaaa
    26041 tcaagggctt gcatcgtatt ggtgcaagcc cttttggttt ttggctgcgc tttgggtgtg
    26101 tctcagaaat gacattcact cgtgagtgtc ggtgtcttgc cgtcatgctg ggtgtgttcg
    26161 tctgccatac tttccgccca aagcagaagg gagcggtgtt gcagcaagac aatccaccat
    26221 catcgggcct gattcggagg gcaatggatg cgctgttgac caccgatcgt gatcagcgca
    26281 ttcgtctttc gatgagcgtg atggcggcct tgctgatgct gtgttgtatt gccgcgctgc
    26341 agctggtggc ggccgcgggt ctggctgatg ccggacatgt gcattggttc agcgtggtgt
    26401 gctcccttgg cctgattgcc gtgtattggc tcattcgcag tggctacagc cagcgctggg
    26461 aagacccggc cctgacgttc ctccagatcg tttcatccgt aacgtgcact gccgtggcct
    26521 atgtgattgc cggatcggcg cgcggcatca cgctgccgat cctggcggtg atcctgattt
    26581 ttggcgtgtt tgggctgtcg ccacggcaga tgctgtttgt gttgttctac ggcattgcag
    26641 tgtttggcgt cgccctgtcg gtggcccaat ggggcctggc tcatgggagc cagccgttgg
    26701 cactgacggc ggcttacatg gtgatgattg tggtgatgct ggcgagttgc acgttcatca
    26761 acctgcgtgt gctggcctcg cgcaagcgca agctggagct cgcgcaagcg gcggccaacg
    26821 agcgcgagcg ggccattcgc gatgaactga ccggcctgca caaccggcgt ttcatgctgg
    26881 agatcatgca cctggaaggc gcgcgggtgc agcgcaacca ccagcccctg ctgatagccc
    26941 agctggatct ggatcatttc aagcacatca acgacaccca cggccatgat gccggggatc
    27001 aggtgctggc cgcgtttgcc cggacggtgg cgggctacat tcggtccacc gatatcctgg
    27061 cgcgctgggg cggtgaggaa ttcgtgttgc tgatggtcaa tacggcggcg gacaacggtg
    27121 tcgctctgct cgagcgtgtg cgtgccgcgg tgcaggagtc agtgctggtg atggcgacgg
    27181 gtgccagtat ccgggtgacc gtttccatcg gtgtggcgca gctgcagacg gggcatgaaa
    27241 cacccattgg cttgctgcgc cgcgccgacg aggctttgta tgcggcgaag gcccaggggc
    27301 gcaaccgcgt cgtccttgca gaccaaacgt tactggaaac gaccgttgcc acgggttgat
    27361 cctgggccgc gcggcggcag cgccgcccgc catgacctgg tcgatgcctt gcgcggcctg
    27421 gccatggtgt ggatgacggt gtttcatttc tgcttcgacc tgagccactt tggctactgg
    27481 cagcaggact tccttggcga tccattctgg accgtacagc gcaccctgat cgtcagcctg
    27541 tttctgttgt gcgccggcat gggtcaggcc gtggcctggc acaggcggct ggcgtggccg
    27601 cggttctggc ggcgctgggg gcagatcgca gcctgtgcgc tcttggtcac ggtcggttcc
    27661 tacctgatgt ttccacgcag tttcatttat tttggcgtgc tgcacggcat ggcggtgatg
    27721 ctgctcatcg cgcgcttgac cgcgggctgg ggacgctggc tgtggccaat gggcctgctg
    27781 gccgtggcct tgccctggtt ggcggggtgg gctttggagg ggccgcttgc cggactggcc
    27841 gagacgttca atggccgtgc gctgaactgg ctcggctggg tgacgcacaa acccttcacc
    27901 gaggactatg tacccctgtt cccctggttg ggcgtgatgt ggtggggcat ggcgggcggg
    27961 caatggctgc tcgctcggcg gccccactgg cttgcctggg ccatgccggg caccggcgtg
    28021 gcgctggcgg tgctgggccg ctacagcctg agctattaca tgctgcacca gccggtgatg
    28081 atgggcgccc tgatgctggt gggctggttc atgcagccgt aaaaaacgcg gccaccaggg
    28141 ccgcgttgtt gggtgcgcag aggcgcggtg cgtcactcca ccttggcctt ggcgcgcagg
    28201 tcttcctgga acttggccag tttttgctgc tgcagctgct gggcaatctg gggcttgacc
    28261 tcgtcgatct tgggcagctg ggcctcgcgc acgtcgtcca ggcggatgat gtgccagcca
    28321 aactggctct tcacgggggt ctgggtcagc ttgccttttt ccagcttgac cagggcttcg
    28381 gtgaactcgg gcacgtagct gctcgggttg gcccagtcca ggtcgccgcc acgggcgccg
    28441 gatccgggat ccttggactg cttcttggcg atctcttcga acttgccgcc cttcttcagc
    28501 gccgcgatga tggccttggc ctcgtcctcc ttttccacca ggatatggct ggccttgtat
    28561 tccttgccgg cgttggcggc agcaaacttg tcgtactcgg cctggatctc ggcgtcggtg
    28621 acggggttgg tcttctggaa gtccacgaac agctcacgga tcaggatggt ctggcgcgcc
    28681 agctccattt gcgccttgta gtcagacgtc gcctccaggc cgcgcttttg cgcttcctgc
    28741 atgaagatct cacgggcgat gacttcttcc ttgatctggt tttccatctc gggagagatg
    28801 gggcggcccg agcgttccac ctgttgtttc agggtttcga cacgttcctt gggaacggcc
    28861 ttgccattga cgatggcgag gttttgtgcg gcaacgggca aagccatcgt gcccagcacg
    28921 gctgcggcca cgaggccgga caagagcgat ttcttcattg gagatccaca gggagaaaac
    28981 aagtctgcgg gccaccggac ccgcagtttc ttagagtgag gctgggttag agagtttcaa
    29041 tggcgatggc gtgcgcgcca tggtcaataa aaatctgcag cgcatcatac acaagcctat
    29101 gctgcgccac cttgcgttgg ccggcgaacc ggggcgaggc aatgcgcacg cgaaagtgcg
    29161 tgccaaagcc cgtgccgttg gcgccggggt ggccttcgtg ctgccagctt tcgtccagca
    29221 cttgcagctg cgtgggcgcc aaaacctcgc gcaggcgcgc ctccatggct tcggcggtga
    29281 tggcgatggg ggtggcggtc atggttgcgc gtcctttgcg gtggtgccgt cttccttcat
    29341 gtggcggctc aggtacaggg cctgggcgac gacgaaggcc agcatcaggc ccatgccgcc
    29401 aaacagcttg aagttgaccc aggtgtcggt gtcaaagttg taggccaccc acaggttgag
    29461 cacgcccatg gcgctgaaaa agccggtcca ggcccagttg agcttgagcc acacgggcgc
    29521 gggcagctcg atttgcgcac ccatcagcga ctggatgaag ttcttgcgaa acagcagctg
    29581 gccgacgagc aatgctccac ccatgagcca gtacagcaca gtgggcttcc atttgatgaa
    29641 ggtttcgctt tgcgccaaca gcgtggcgcc gccaaacagc acgatcaccc ccaggctgag
    29701 ccattgcatg ggctcgacct tgccgtgctt gaagtggatg taggcgatct gcgcgatggt
    29761 ggcgacgatg gccacgccgg tggccacgta gatgccccag gccttgaagg ccacgaagaa
    29821 caggatgatg gggaaaaaat cgatgagcag tttcatggcg ccgggggttc aaaggcgagc
    29881 gaggcggagt tcatgcagta gcgcaggccg gtgggggccg ggccgtcggg aaagacatgg
    29941 cccaggtggg ccccgcattg tgcacatacc gtctcgacgc gccgcatgcc gtggctcctg
    30001 tcctcgattt cctggatggc gccgggcagg gccagcgaaa agctgggcca gccgcaaccc
    30061 gcgtcgaact tggttccgga gtcaaacagc ctggcgccgc agcagatgca gtggtagctg
    30121 ccgtcggccc aatgccgctc gtacttgccg gtgaatgggc gctcggtggc ggcatggcgc
    30181 gtgacctgga aggccgcggg ctcggcgccc ttgtcacgca gcagggcctg ccattccgcg
    30241 tcggttttct gtattgcgta ggtcatgaag agcaactaat ggaaagggag gaggcccagt
    30301 cgggcgggaa gtcggcatag gcctcgaacc ccgggtgttc gtcaaagggg cgcgccagca
    30361 gccgctgaag ggtttggagt actgaaaggt cgccaagttt cgcagcccgt atggcctgtt
    30421 cggccaggtg gttgcgcagc acgaagcagg ggttggtttt cagcatcaaa tcggccgcca
    30481 tggctttatc atcaagggcc agtagctctt tatattgtag cgaccaggcg tcgaaggcgg
    30541 tgcgatcggc gaacaggtcg cgcacgggct cgtccttgcc gtcggccacg gcgcgtgaca
    30601 ggcggcgcca gaagatggtg tagtccacgc ggttcttggc cagcagctgc tgcaggctat
    30661 cgaccagggt ttcgtcgccc tcgcgtgccc ggcgcagccc gagcttggcg cgcatgtgcg
    30721 ccatgaactg ttctatgaac accggttcgt aaccgtccag cgcttgctgc gcctgctcca
    30781 cgtcgcggat cagcggcagc agcgccttgg ccaggcacag caggttccag taggccacgc
    30841 cgggctggcg gttgaaggcg tagcggccca ggtggtcgct gtggttgcag atatggttgg
    30901 ggtcgaaggc gtcgaggaac tggaacggcc cgtagtcgat ggtcaggccc aggatgctca
    30961 tgttgtcggt gttcatcacg ccgtggcaaa agcccacggt ctgccactgc gccagcagcg
    31021 cggccgtgcg ttcgctcacc gcctgcagca gctgcgcata ggggttgctc gtgccgctgc
    31081 actgcgggta gtagcgctcg atgacgtagt cggcgagctg gcgtagttcg gcctcctgcc
    31141 cgcgcgcggc gaaatgctcg aaatggccga agcggatgaa gctgggcgcc acacgcgtga
    31201 ctacggcggc gctctccagc tcctcgcgct gcacgggcgc gggcgagccg gtcacgcaca
    31261 gggcgcgcgt ggtgggaatg cccagggcgt acatggcctc actacagaga aattcgcgga
    31321 tcgacgaacg cagcacggcg cggccgtcgc ccatgcgcga gtagggcgtg cgcccgcagc
    31381 ccttgagctg cacctcctgg ccgcccgccg tctcgcccag caggatggcg cgcccgtcgc
    31441 cgagctggcc ggcccagacg ccgaactggt ggccgctgta tacgctggcc agcggccggc
    31501 tgccgggcag aaggacattg ccggtgaaga cctgcagggc atcgtcgcgc tgcggccagc
    31561 cgacgggcag acccagcagc gtgcccacgt cggcgctggt gcccacccag tacggggcgg
    31621 gcaggggcgt gggacgcagc tcggtatgaa aggccgggcc cagcgcggca aagccgtcgt
    31681 gccagccggc cagcaggggc tgggcaggtg ttgttgcggg aggggggcaa accgtggatg
    31741 caggcatgcg gggattgtcc ttgcaggccg atgtcccgca gtggacaaag gccttgcgtc
    31801 tggcgtgccc acgcggggtt tttcctgatg gccgggagga tgacggcacc gggttcgccg
    31861 ctaggatggt gcggctttcc atccaccttc tccgaggagt atccatgctg ggcctgatgc
    31921 agaaccaacc tctcctgatc tcgttgctga tcacctttgc cgagcgcaac catggcgatg
    31981 gcgagatcgt ctcgcgccgg gtggagggtg atattcaccg ctgcacctac cgcgacttgg
    32041 cacggcgcgc gcgccagctg gcgaataccc tcgatggcat gggcctgcag ttcagcgacc
    32101 gcgtggccac cctggcctgg aatggctacc gc
//
LOCUS       DAOMTQ010000010        31374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_109_length_31374_cov_26.680162, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000010 DAOMTQ010000000
VERSION     DAOMTQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 31374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31374
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109_length_31374_cov_26.680162"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..960)
                     /gene="trpA"
                     /locus_tag="PK518_02125"
     CDS             complement(142..960)
                     /gene="trpA"
                     /locus_tag="PK518_02125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRM47736.1"
                     /translation="MSHPQRIAATFAELQAKGRKALIPYVTAGFPFADITPALMLGMV
                     EAGADVIELGVPFSDPMADGPVIQKAGEKALALGIGLAQVLDMVRTFRQRNDSTPVVL
                     MGYANPVERYEQRHGQGSFARDAGAAGVDGVLVVDYPPEECEQFAADLRAHGIDLIFL
                     LAPTSTPERMQQVAKVASGYVYYVSLKGVTGSGALDTAAVEAMLPRIREHVKIPVGVG
                     FGIRDAATAQAISRVADAVVIGSRIIGLIEDQPHERVVGVTVDFLRQVRKALDA"
     gene            complement(957..2225)
                     /gene="trpB"
                     /locus_tag="PK518_02130"
     CDS             complement(957..2225)
                     /gene="trpB"
                     /locus_tag="PK518_02130"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRM47737.1"
                     /translation="MSEYHQPDASGHFGPYGGSFVSETLTHAIDELRQAYARYQHDPE
                     FLAEFHYELAHFVGRPSPVYHAARTSREIGGAQIYLKREDLNHTGAHKINNVIGQAML
                     ARRMGKPRIIAETGAGQHGVATATICARYGLECVVYMGAEDVKRQSPNVYRMKLLGAT
                     VVPVESGSKTLKDALNEAMRDWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGQECL
                     TQMPAMLAEQKIAAEQPDAVVACVGGGSNAMGIFHPYIPFENTRLIGVEAAGEGLDSG
                     KHSASLQRGSSGVLHGNRTFILQDENGQITETHSISAGLDYPGVGPEHAWLQEIGRAE
                     YVGITDAEALAAFHHLCRTEGIIPALESSHAMAYAMKLAKTMGPDQSILVNLSGRGDK
                     DIGTVADLSGVDFYDRPSMRGLTVKGGPAA"
     gene            complement(2232..2954)
                     /locus_tag="PK518_02135"
     CDS             complement(2232..2954)
                     /locus_tag="PK518_02135"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRM47738.1"
                     /translation="MSSAPPSSIPVRTRIKICGLTREQDVDAAVAAGADAIGFVLYPK
                     SPRAVSPARAAELARRLPPFVTPVLLFVNAKATEVIAASAYVSGATVQFHGDESPADC
                     WAASDKGRIPFLRAARIPLGDGAARFDLVKYAQDYSHARAILLDAHVEGYGGGGKAFN
                     WSLLPPSVSSHLVLSGGLTPANVTDGISQVRPRCKTLAVDVSSGVELDGPDGRPLKGI
                     KDAGKIQRFVAAVRAADAAPAI"
     gene            complement(2951..3775)
                     /gene="truA"
                     /locus_tag="PK518_02140"
     CDS             complement(2951..3775)
                     /gene="truA"
                     /locus_tag="PK518_02140"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM47739.1"
                     /translation="MRVALGVSYNGQAYSGWQSQPGGNTVQDHLEAALGRFATHKVAT
                     ICAGRTDAGVHGLMQVVHFDTALERAPFSWVRGTNTFLPADIAVQWAQPVPDSFHSRA
                     CAVARRYAYVLLQSPVRPSVDAGRVGWVFHALDEEAMQAAVQHLLGEHDFTSFRASAC
                     QAKSPIKTLQRISITRRSPPAGESAGDHGCAPCYWRFEFEGNAFLHHMIRNIMGCIVA
                     IGQGLYPPDWMRQVLEARSRDAAAPTFSPDGLYFQGPVYAAEWGLPTRTAAYDWLP"
     gene            complement(3843..6503)
                     /locus_tag="PK518_02145"
     CDS             complement(3843..6503)
                     /locus_tag="PK518_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HRM47740.1"
                     /translation="MHRWKFSALATAAILSAGLLGTDANALTLGRVNVQSALGEPLRA
                     EIELPQITAAEAESLRVSTANAAAFRSQGLEYSPTVGNVQVQVHRRADGSMVLRLSSS
                     RPINEPFVDLVLDATWSSGHIVRSYTMLFDPPAQAAKPAEVTVAPQLAAPRSESAPTP
                     PRTSSARPAAPPAAAPAAAAPRPTPRVAPAPSRAPAATEAGAGSDDTVTVRPGDTAGR
                     IANAHRPASVSLDQMLVAMAQANPKAFVDGNVNRLRSGAVLQMPSESAAQATPASEAR
                     QIVAAQSRDFNEFRRRLAGAAPTAQVAAAERSASGSVQAQVQEAKPAAASPDKLTLSK
                     GGVQARKADEQMARDKQADQNTARMAELSKNIAELNQLSGASTQPATPAADTSAAAPN
                     AAGSQSAVAVPAPAGLPGAVAPAQTNANPEAPATAEQAPTAPQATPQEAAPAPAAAAA
                     PAPKPAPPPETYEEPSFLGSLTEDPLLAGGALALVLALLGYGGYRVAQSRRQQAQLDS
                     SFQDSGNPDSFFGASGGQHVDTADSSLTTGASSMSYSPSQLDVGGDVDPVAEADVYLA
                     YGRDLQAEEILKEAMRHNPERVAIPVKLAEIYAKRQDRKALESAASDVFRLTDGQGPD
                     WSRVADLGRTLDADNALYQPGGRPAAGPDGEGDIAPLSNFPSTVGVGAPTNASTGPDS
                     VLPDLDLDLDLDLDLHDAPPAPAPAPSAFAMAAAASELAATQGAAATPAVSEDLPAAL
                     DAIDLELPDAGPEAAPRSAEPTAVASLTTEPAPLDFDTDNLLLADSAATPLAASDAKD
                     SAPAALEFDLGDLNLDLSLPAAPTAAAQADAGAAEPLPDDPLTTKLALAEEFNTIGDS
                     EGARTLVEEVIAESTGELKARAQRLLAELG"
     gene            complement(6777..7889)
                     /gene="asd"
                     /locus_tag="PK518_02150"
     CDS             complement(6777..7889)
                     /gene="asd"
                     /locus_tag="PK518_02150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRM47741.1"
                     /translation="MSKQLVGLVGWRGMVGSVLMDRMQAEGDFDLIEPVFFSTSNAGG
                     KAPAMARTHTELKDANDVAELAKCDIIITCQGGDYTKEVFPKLRAAGWQGHWIDAASA
                     LRMEGDAVIVLDPVNDGLIRQKLAAGGKNWIGGNCTNSILLMGLAGLFKAGLVDWVSS
                     MTYQAASGGGANHMRELLKGMGVVHGAVADELATPASAILEIDRKVASTIREDVPTEH
                     FGAPLAGGLIPWIDAQLDNGQSKEEWKGQAEVNKILGTAATIPVDGLCVRIGAMRCHS
                     LALTLKLKKDIPLAQIEALIKDGNPWVKFVPNERAITVKELTPAAITGGLEVGVGRVR
                     KLNMGPEYVSAFVIGDQLLWGAAEPLRRMLRILLSA"
     gene            complement(8156..9226)
                     /gene="leuB"
                     /locus_tag="PK518_02155"
     CDS             complement(8156..9226)
                     /gene="leuB"
                     /locus_tag="PK518_02155"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRM47742.1"
                     /translation="MKIAVLPGDGIGTEIVAEAVKVLKALDLKFEMETAPVGGAAYEA
                     AGHPLPESTLALAKSADAILFGAVGDWKYDKLDRPLRPEQAILGLRKALGLFANFRPA
                     ICYEQLTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRTAVDGHFPGAEEAFDTM
                     RYSRPEIERIARVAFEAARKRSKRVTSVDKANVLETFQLWKDVVTEVHKDYPDVALDH
                     MYVDNAAMQLVKAPKAFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLNDKKQGLYE
                     PSHGSAPDIAGKGVANPLATILSAAMMLRFSLNQPEAADRIETAVKKVLAQGLRTPDI
                     YSDGTTKVGTVQMGDAVVNALR"
     gene            complement(9300..9953)
                     /gene="leuD"
                     /locus_tag="PK518_02160"
     CDS             complement(9300..9953)
                     /gene="leuD"
                     /locus_tag="PK518_02160"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRM47743.1"
                     /translation="MQKFTVHQGLVAPMDRENVDTDAIIPKQFLKSIKKTGFGPNLFD
                     EWRYLDQPGEPGVPESARKPNPDFVLNQPRYKGASILIARKNFGCGSSREHAPWALDQ
                     YGFRAIIAPSFADIFFNNCFKNGLLPIVLPESVVAQLFDEVAAFPGYELKIDLERQVV
                     IRPQGEEIAFDVLPFRKYCLLNGFDDIGLTLRHKDKIAAFEAERLATKPWLAHTMAG"
     gene            complement(9978..10103)
                     /locus_tag="PK518_02165"
     CDS             complement(9978..10103)
                     /locus_tag="PK518_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HRM47744.1"
                     /translation="MKPSFSIILIGLALTLAACNTIKGIGQDVQSAGGAIERAAR"
     gene            complement(10127..11548)
                     /gene="leuC"
                     /locus_tag="PK518_02170"
     CDS             complement(10127..11548)
                     /gene="leuC"
                     /locus_tag="PK518_02170"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRM47745.1"
                     /translation="MGRTLYDKIFDEHVVHTEEDGTAVLYIDRHLVHEVTSPQAFEGL
                     REAGRKVWRTSSIVATADHNTPTTGWERGYDGIADPISKEQITTLDKNIAASGAAAFF
                     PFLHKRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQ
                     TLLAKKAKNMLVRVNGHVAPGITAKDIVLAIIGKIGTAGGTGYTIEFAGQAIRDLSME
                     GRMTVCNMAIEGGARAGLVAVDEKTIAYVKGRPLAPTGVEWDQAVQYWQTLHSDADAQ
                     FDAVVELNGAEIVPQVTWGTSPEMVLGVDARVPDPDKEKDANKRGAMERALAYMALTP
                     GKPLQDIFVDKVFIGSCTNSRIEDMREAAALVKKLGQKVAKNIKLAMVVPGSGLVKAQ
                     AEAEGLDKIFTAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTH
                     LVSPAMAAAAAIHGHFVDIRKFA"
     gene            complement(11578..12519)
                     /locus_tag="PK518_02175"
     CDS             complement(11578..12519)
                     /locus_tag="PK518_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM47746.1"
                     /translation="MKSSERSFARRIDLTSLQLFVAVCELGSIGRAAEREFIAASAVS
                     KRLSDLEAAVDTALLYRHSRGVTLTPAGESLLHHARTVLFGLERMQGELSEYAEGVRG
                     HVRMHANISAIVQFLPEDLGHFARAHSQIKIDLQEHLSPEVLQAVQEGAADLGICSLG
                     NASQGNGAAELQSRPYRSDRLVLVMPQTHALSAREAIKFDEVLDWDIVGLHAGSSISL
                     AMRAAAAQAGRPLRQRIQVTSLDTMCRMIDNGLGVGLLPDRAFALMQGLGRLAAVRLD
                     EPWAERELRLVARDFDALPVTARLLVEHLAPKISPSP"
     gene            complement(12702..14066)
                     /locus_tag="PK518_02180"
     CDS             complement(12702..14066)
                     /locus_tag="PK518_02180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM47747.1"
                     /translation="MPVSLTFLGGAGTVTGSKYLVRHGGQVLMVDCGLFQGYKLLRQR
                     NWRPLPVAPQEIDAVILTHAHLDHSGYLPLLARDGFGGPVFCTSGTRALCAILLPDSG
                     HLQEEEAAYLNRHRLSKHEPALPLYTRLDAQHSLALLQVQPVHQQFEPLPGWRATFTR
                     AGHILGAASVLLEVGGRRILFSGDLGRPDDLLMCAPDAPPAADTVLIESTYGDRGHPS
                     GSVLDELGPPLARLAARGGVAVVPVFAVGRAQTVLHAIARLKARGELPTGLPVYLDSP
                     MAVSATELFQNHVGEHCLSRADLQAMARGTTLVHSVDQSKALARLHGPRVILSASGMA
                     TGGRVLHHLALHAGDHRNMIILTGHQAGGTRGARIAAGEKTIRILGREVQIRAEVVQL
                     ATASAHADGNQLLDWLRALPAAPRRIYVVHGELPAADQLRQRVEHELLWPAEVPEHGD
                     TVAF"
     gene            complement(14172..15029)
                     /locus_tag="PK518_02185"
     CDS             complement(14172..15029)
                     /locus_tag="PK518_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRM47748.1"
                     /translation="MTQTQDIQQIVKEKYGEAARRVTQGGAASSCRGAVAAPTNSCCA
                     TGPLAGTDPITRDLYDAVQTGGLPEAAVLASLGCGNPTALAELRAGEVVLDLGSGGGI
                     DVLLSAKRVGPTGKAYGLDMTDEMLALARDNQRQAGATNVEFLKGEIEHIPLPDASVD
                     VIISNCVINLSADKDRVLAEAFRVLKPGGRFAVSDVVTRGAIRPEIRASAMAWVGCVA
                     GALDEDDYRHKLAAAGFSDIGVEPTRIYTAQDAREFLSANGLDTSVVSDEMDGKFLSA
                     FIRAVKPAH"
     gene            complement(15026..16123)
                     /gene="arsB"
                     /locus_tag="PK518_02190"
     CDS             complement(15026..16123)
                     /gene="arsB"
                     /locus_tag="PK518_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRM47749.1"
                     /translation="MSQLTRKLSFLDRYLTLWIFAAMALGLALGWLVPDIEAIIGRYQ
                     VGSTNIPIAIGLVLMMYPPFAKVRYEDLPAVWRDTRVLWLSLLQNWVIGPVLMFVLAV
                     VFLPDQPEYMAGLILIGLARCIAMVIVWNDLARGSTEYAAALVAFNAFFQVLFYSVYA
                     WLFLTVLPPLFGLGGSVVAISMGQIAESVFIYLGIPCIAGLLTRWVLLRFVSKDWYHT
                     RFVPRIAPLTLVALLLTIVVLFSLKARTVLALPLDVLRIALPLLIYFVLMFVLSFAMG
                     RRAGADYPRCVTLSFTAASNNFELAIAVAVAVYGIHSGAAFAAVIGPLVEVPVMIALV
                     ALALRWQRRWFAGEKASPSVAQTCTPIGEPR"
     gene            complement(16141..16626)
                     /locus_tag="PK518_02195"
     CDS             complement(16141..16626)
                     /locus_tag="PK518_02195"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRM47750.1"
                     /translation="MTTHILILCTHNSARSVLAEAMLNHLAAAQGLDVRAHSAGSAPS
                     GRVNPQALEVLAAAGVATQGLRSKSWDEFSAADAPPMDIVITVCDSAAEEACPLWPGN
                     PVRLHWGYADPSSAPGGAAGQRAAFEHTRQTLAERLQRLLALPLASRGAAALHTELSR
                     L"
     gene            complement(16631..16951)
                     /locus_tag="PK518_02200"
     CDS             complement(16631..16951)
                     /locus_tag="PK518_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM47751.1"
                     /translation="MEEQSVLKALTALSQAHRLRVFRALVVAGQAGLTPGQIAEAQQI
                     PAATLSFHLKELVQAGLLTQERQGRSLIYRADFAQMNALLAFLTENCCQGDACAATPS
                     APCC"
     gene            complement(17038..18186)
                     /locus_tag="PK518_02205"
     CDS             complement(17038..18186)
                     /locus_tag="PK518_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRM47752.1"
                     /translation="MSSTPTPWDPQAPEVQRDQRAAFDQLRARCPLAHSASQHTTVFG
                     HPEVMQVLQDHATYSSAVSQHPAVPNGYDQPQHTRYRQALDHFFAPERMARFEPVCRA
                     LSAATVQEALQLGGSLEITQEFALPFAVRVQSAFLGWPTLLQDELLQWLRRSQAATRS
                     GERAQTAAVAQEFEDLVQRVRQARQGVPATQDISSELMHTRVDGRLLDLRELASVLRN
                     WTVGEVGTIAASIGILLHWLASHPDWQQRLRAQPALLGAAIDEVLRIDGPLVGNRRIT
                     TRPVTLGGCPLPAGERVAINWIAANRDPRVFDQPGEFRLERDPALNLLYGAGIHVCPG
                     APLARLELRVLIEEVLARTQAITLSPSHAPTRAVAPDAGYATLHMRLQ"
     gene            complement(18199..19656)
                     /gene="ubiD"
                     /locus_tag="PK518_02210"
     CDS             complement(18199..19656)
                     /gene="ubiD"
                     /locus_tag="PK518_02210"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HRM47753.1"
                     /translation="MAQLEAAGELRRIAAPVSPHLEMTALADRVLRAGGPALLFENPT
                     GHAMPVLANLFGTPARVARALGVAGLPEIRAFGEVLASLKEPEPPRGMKDLWGRRELV
                     KTLWSMAPSNVRRPPCQELVVEGDEVDLSRLPIQHCWPGDVAPLVTWGLTITRGPHKR
                     RQNLGIYRQQVIGKNQLIMRWLSHRGGALDFAEHCRQHPGQPYPVAVAIGADPATILG
                     AVTPVPDTLSEYQFAGLLRGARTEVADALGVPLQVPAQAEIVLEGHIHPDAQHPSGWQ
                     HALEGPYGDHTGYYNECAEFPVFTVERITQRRDAIYHSTYTGKPPDEPAMLGLALNEL
                     FVPLLQRAFPEIVDFYLPPEACSYRMAVVSIKKAYAGHARRVMMGVWSHLRQFLYTKF
                     VVIVDDDIDVRDWKEVIWAMTTRMDPVRDTMLIANTPIDYLDFASPVSGLGGKMGLDA
                     TNKWPGETQREWGRTMAMDAAVKARMDTLADDLGL"
     gene            complement(19737..20321)
                     /locus_tag="PK518_02215"
     CDS             complement(19737..20321)
                     /locus_tag="PK518_02215"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRM47754.1"
                     /translation="MAEPRRIIVGISGASGAVYGVALLQALRAMPGVQTHLVVSDAGW
                     LNIQHETALSREQVLALGHQAHDNASIGASIASGSFGAQAMVVAPCSMRTLAAIAHGL
                     ADNLLTRAADVILKERRRLVLLARETPLHLVHLRNMTTVTEMGGIICPPVPAFYQRPQ
                     TVEDIVQHSVARSLDLLGLEHALAPRWQGLHGQS"
     gene            complement(20314..20709)
                     /locus_tag="PK518_02220"
     CDS             complement(20314..20709)
                     /locus_tag="PK518_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HRM47755.1"
                     /translation="MTDPAPAPLPPLVYSCSGCSSAAQLANHVAVQMDRRGLAEMSCI
                     AGVGGDVPKLVQLAKSGRPVIALDGCPLECVRNSLARHSVTPARHYQLHKYGVKKRNH
                     QDFDPAQADEVLAHVQADLAAAPLVPAHG"
     gene            complement(20706..21224)
                     /locus_tag="PK518_02225"
     CDS             complement(20706..21224)
                     /locus_tag="PK518_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRM47756.1"
                     /translation="MTTTPPAPFTLPAPVGALLSRLPAYPGSMLLVTAINFALAKQLP
                     QDVKDILRAKRLAIRVRDARVAFDFSWNGQRFVPCKPQAETDLAISANAQDFLLLAQR
                     RQDPDTLFFNRRLVMEGDTELGLVVKNALDALELPVLDPREWTPRAVLERKAPQLLRQ
                     LPPLPRFLRGRP"
     gene            complement(21244..22143)
                     /locus_tag="PK518_02230"
     CDS             complement(21244..22143)
                     /locus_tag="PK518_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM47757.1"
                     /translation="MKLALGPLQYYWHRDTVFEFYQQMAESPVDIVYLGEAVCSRRRE
                     LRQSDWLDIARMLRDAGKQAVMSTMVLLESTSDVADMHKIVRDEEFLIEANDMGAVHK
                     LAGKRPFVAGPQLNLFNADSLAWMAGLGASRWVMPLEMKHSDLAVLLQTRPAGLETEV
                     FAYGRMPLAFSARCFTARHYNLPKDDCGFRCIEHPDGQLLQTREKESFLVINGIQTQS
                     AKVHNLVGDMPALRAAGVDILRISPQSQHTDKIIAAFDAARRAAQPNPAALQAMRPFM
                     PEASCNGYWHGRPGLDLIEPALA"
     gene            complement(22289..23341)
                     /locus_tag="PK518_02235"
     CDS             complement(22289..23341)
                     /locus_tag="PK518_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRM47758.1"
                     /translation="MLSPSTPIETGTSPARTAPELVCPAGSLPALKTAVDHGADCVYL
                     GLRDATNARNFAGLNFDEAAIQTGIAYAHQRGRKVLMALNTYPQASNSTPWRSALDRA
                     SAWGIDAVILADPGLMRYANEHHPDLRLHLSVQGSATNADAINLYQQQFNIQRAVLPR
                     VLSLEQVKQVTQRTDVEIEVFGFGSLCVMVEGRCALSSYATGESPNTHGVCSPPKSVR
                     WQETPRGLESRLNGVLIDRYAQGENAGYPTLCKGRFDVGDESNYYALEEPTSLNTLEL
                     LPQLVKMGVRAFKIEGRQRSPAYVAQVTKVWREAIDHCMAQGHLYAPKTAWMASLDQV
                     AEGQQHTLGAYHRPWK"
     gene            23583..24953
                     /locus_tag="PK518_02240"
     CDS             23583..24953
                     /locus_tag="PK518_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRM47759.1"
                     /translation="MPKTLIEPFRIKSVEPIRMTTAAERARLLEEARLNVFKLRAEDV
                     LIDWLTDSGTGAMSARQWGAIMEGDESYAGARSFFRLEKVIQDITGMQYFVPTHQGRA
                     AEKVLFTAVCKQGDLVPNNCHFDTTRGNLEYLGVEALDLVEAVGLQPATIAPFKGNID
                     LARAEEVLKKEGQRIPFGMLTVTNNTGGGQPVSMANIRAYAQLLKKYGKPFIMDVCRF
                     AENAMFIKMREPGYENTSIKAIAQEMFSYADGATMSAKKDGMVNIGGFIVLRSDEWLD
                     EVRSDLIMMEGFPTYGGMAGRDLEALAVGLEEGLDEDYLRYRLRTAEYLGEKLDAAGV
                     GFVRPTGGHAVYIDAKSVLPNMSVEHYPAWALCNALYLEGGIRGVEIGSVMFGKRLQD
                     GSETYHSMELVRLAFPRRMYTQSHFDYAAEVIAEVKQKAASIRGVKIIKQPKFLRHFT
                     CECAWV"
     gene            complement(24969..26781)
                     /locus_tag="PK518_02245"
                     /pseudo
     CDS             complement(24969..26781)
                     /locus_tag="PK518_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            complement(26795..27025)
                     /locus_tag="PK518_02250"
     CDS             complement(26795..27025)
                     /locus_tag="PK518_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRM47760.1"
                     /translation="MSTLTIRLPDDTAQRLKNLAHTRGLSINKLMEELSTQAIAAFDA
                     ETRFRTLAASGDAAQALAILDRLDQQDADSTH"
     gene            complement(27022..27441)
                     /locus_tag="PK518_02255"
     CDS             complement(27022..27441)
                     /locus_tag="PK518_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRM47761.1"
                     /translation="MRTIVLDTNVFVSALRSGGGASRQVLRRILQGQATPLFGNTLWQ
                     EYQDLLGRDVWTSATTPEERLQVLAALARAGRWVQVYYGWRPNLRDEGDNHLIELAVA
                     GNAQAVVTHNVRDLARGELAWPRLRILTPAQFLEEQP"
     gene            complement(27751..27909)
                     /locus_tag="PK518_02260"
                     /pseudo
     CDS             complement(27751..27909)
                     /locus_tag="PK518_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011204925.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(28011..29144)
                     /gene="dnaJ"
                     /locus_tag="PK518_02265"
     CDS             complement(28011..29144)
                     /gene="dnaJ"
                     /locus_tag="PK518_02265"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRM47762.1"
                     /translation="MSKRDYYEVLGVPKNASEDDIKKAYRKLAMKHHPDRNQGDAAKN
                     AEEKFKEAKEAYEMLSDGQKRAAYDQYGHAGVDPNMRGGMGGTEGFGGFAEAFGDIFG
                     DMFGGQRGGRGGRQVYRGNDLSYAMDVTLEEAAHGKDAQIRIPSWESCDTCHGSGAKP
                     GTSAKTCGTCQGSGAVQMRQGFFSVQQTCPHCRGTGKIIPEPCTSCHGQGKIKKQKTL
                     EVKIPAGIDDGMRIRSTGNGEPGTNGGPPGDLYIEIRVRKHDIFERDGDDLHCQVPVS
                     FITAALGGEIEVPTLAGKAAIDIPEGTQAGKQFRLRGKGIKGVRASYPGDLYCHIVVE
                     TPVKLTEHQRKLFKELDESLKKGGGKHSPSTDSWTDRLKSFFS"
     gene            complement(29240..31183)
                     /gene="dnaK"
                     /locus_tag="PK518_02270"
     CDS             complement(29240..31183)
                     /gene="dnaK"
                     /locus_tag="PK518_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRM47763.1"
                     /translation="MGKIIGIDLGTTNSCVSIMEGNTTRVIENSEGARTTPSIIAYQE
                     DGEILVGASAKRQAVTNPKNTIYAVKRLIGRKFDEKEVQKDIDLMPFTITKADNGDAW
                     VEVRGQKLAAQQISAEILRKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKQDKADRKIAVYDLGGGTFDVSIIEIADVDGEKQFE
                     VLATNGDTFLGGEDFDQRIIDYIIGEFKKDQAVDLSKDVLALQRLKEAAEKAKIELSN
                     SAQTDINLPYITADATGPKHLNIKLTRAKLESLVDDLIERTIAPCRTAIKDAGISVSD
                     INDVILVGGMTRMPKVQDKVKEFFGKEPRKDVNPDEAVAVGAAIQGQVLSGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMITKNTTIPTKFAQTFSTAEDNQPAVTIKVFQGEREIAN
                     ANKLLGEFNLEGIPPSARGTPQIEVSFDIDANGILHVGAKDKGTGKENKITIKANSGL
                     SEAEIQQMVKDAELNAAEDHKKLELVQARNQGEAAVHTVHKSLAEHGDKLDAGEKEKI
                     EAAVKDLEAALKGEDKAAIEEKTTALMTASQKLGEKMYADAQAAQGAGAEAAGAQAAA
                     GASAGAAAQDDNVVDAEVKEVKK"
     gene            31182..>31374
                     /locus_tag="PK518_02275"
     CDS             31182..>31374
                     /locus_tag="PK518_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47764.1"
                     /translation="MFFSEGFFKNVDDGYVWLAGLAFKGASVLRVAFCVLRFAFCVNT
                     AQRSTLNLTWAPPPSPAPGA"
BASE COUNT         4747 a         9955 c        11078 g         5594 t
ORIGIN      
        1 ccgcagccta tgaaactggc gcgacccggg cgagggccgc cgtgcaaggg ccgccccgcc
       61 gcactggcgg cgtccccctt cccgcgcatc gcgcgagaga agggggaagc ggcgcagccg
      121 ctcagggggt tgcgtcccga attatgcgtc cagcgccttg cgcacctggc gcaggaagtc
      181 cacggtgacc ccaaccaccc gctcgtgcgg ctggtcttcg atcaggccga tgatgcggct
      241 gcctatgacc acggcgtcgg ccacgcggct aatcgcctgg gccgtggccg cgtcgcggat
      301 gccgaagccc acgccgacag ggatcttcac atgctcgcgg atgcgcggca gcatggcctc
      361 caccgctgcg gtgtcgagtg cgcccgagcc cgtcacgccc ttgagcgaga catagtacac
      421 atagccgctg gcgaccttgg ccacctgctg catgcgctcg ggtgtggagg tcggggccag
      481 caaaaagatc aggtcgatgc cgtgggcgcg caggtcggcg gcgaactgct cgcattcctc
      541 gggcgggtag tccacgacca gcacgccatc gacgccagcc gcgccggcat cgcgggcaaa
      601 agagccctgg ccatggcgct gctcgtagcg ctccaccggg ttggcgtagc ccatcagcac
      661 cacgggcgtg ctgtcgttgc gttggcgaaa ggtgcgcacc atgtccagca cctgggccag
      721 gcctatgccc agggccaggg ctttttcgcc cgccttctgg atcaccgggc cgtcggccat
      781 ggggtcggaa aagggcacgc ccagctcgat cacgtcggcg ccggcctcga ccatgccgag
      841 catcagggcg ggcgtgatgt cggcgaacgg gaagccggcc gtcacatagg ggatgagggc
      901 cttgcgaccc ttggcctgca gttcggcaaa ggtggcggca atgcgttgtg ggtggctcat
      961 gcagcgggcc ctcccttgac ggtcaaaccc cgcatcgagg ggcggtcata gaaatcgacg
     1021 cccgacaggt cggccaccgt gccaatgtcc ttgtcgccgc ggccggacag gttcacgagg
     1081 atggactgat cggggcccat ggtcttggca agcttcatgg cgtaggccat ggcgtggctg
     1141 gattcgaggg ccgggatgat gccctcggtg cggcacaggt ggtggaaggc ggccagcgcc
     1201 tcggcgtcgg tgatgcccac gtactcggcg cgcccgatct cttgcagcca ggcatgctcg
     1261 gggccgacgc cggggtagtc caggccggcg ctgatgctgt gcgtctcggt gatctggccg
     1321 ttttcgtcct gcaatatgaa ggtgcggttg ccgtgcagca cgccgctgct gccccgctgc
     1381 aggctggccg aatgcttgcc gctatcgagc ccctcgccgg cggcctcgac gccgatcagg
     1441 cgcgtgttct cgaacgggat ataggggtgg aagatgccca tggcattgct gcccccgccc
     1501 acgcaggcga ccacggcgtc gggctgctcg gcggcaattt tttgctcggc cagcatggcc
     1561 ggcatctgcg tcaggcattc ctggccgatc acgctctgga agtcgcgcac catcatcggg
     1621 taggggtggg ggccggccac ggtgccgatg atgtagaagg tgttgtccac gttcgccacc
     1681 cagtcgcgca tggcctcgtt gagggcgtcc ttgagcgtct tgctgccgga ttcaaccggc
     1741 acgacggtgg cgcccagcag cttcatgcga tagacgtttg ggctttggcg cttgacgtcc
     1801 tcggcgccca tgtagaccac gcattccagg ccgtagcgcg cgcagatggt ggccgtggcc
     1861 acgccgtgct ggccggcgcc ggtctcggca atgatgcgcg gcttgcccat gcgccgggcc
     1921 agcatggcct ggccgatgac gttgttgatc ttgtgcgcgc cggtgtggtt taggtcctcg
     1981 cgcttcaagt agatctgcgc gccgccgatc tcgcggctgg tgcgcgcggc gtggtagacg
     2041 ggcgaggggc gaccgacgaa atgcgccagc tcgtaatgaa attcggccag gaactcaggg
     2101 tcgtgctggt agcgcgcata ggcctggcgc aattcatcga tggcatgcgt cagcgtctcg
     2161 ctgacgaagc tgccgccata ggggccaaag tggcccgagg catcgggttg gtggtattca
     2221 gacattcgga ttcatatggc tggtgctgcg tcggccgcac gcacggcggc gacgaagcgc
     2281 tggatcttgc cggcgtcctt gatgcccttg aggggccggc catcggggcc gtcaagttcc
     2341 acgccggagc tgacatcaac cgccagcgtc ttgcaacgcg ggcgtacttg gctgatgcca
     2401 tcggtcacgt ttgcaggcgt gagtccacca gacaaaacga ggtgagagct gacgcttggt
     2461 ggaaggagtg accaattgaa tgccttgccg ccgccgccat aaccttcgac atgggcgtcg
     2521 agcaggatgg cgcgggcgtg agagtagtcc tgcgcgtatt ttacgaggtc aaaccttgcc
     2581 gcaccgtcgc ccaggggtat gcgcgcggcg cgcaggaatg gaatgcgccc tttgtcgctg
     2641 gcggcccagc agtccgcggg cgattcatca ccatgaaatt gcactgtagc gcccgataca
     2701 taggcgcttg cagctatcac ttcagtagct tttgcgttga cgaacagcag taccggcgtg
     2761 acgaagggcg gcaggcgccg ggccagctcg gcagcgcgcg cagggctgac tgcgcgcggg
     2821 ctcttggggt agagcacgaa accgatggcg tctgctcccg cggccacggc ggcgtccacg
     2881 tcctgctcgc gcgtcaggcc gcagatcttg atgcgggtac gtacgggtat ggaagaggga
     2941 ggggcgctgc tcatggcagc caatcatacg cagccgtgcg cgtgggcagg ccccactcgg
     3001 cggcgtatac cggcccctga aagtacaggc catcgggcga gaaggtgggc gccgccgcgt
     3061 cgcgcgaacg tgcctccagc acctggcgca tccagtcggg cgggtacagc ccctggccta
     3121 tggcgacgat gcagcccatg atgttgcgga tcatgtggtg caaaaaagca ttgccctcga
     3181 actcgaagcg ccagtagcag ggcgcgcagc catggtcgcc ggcggattct ccggcgggcg
     3241 ggctgcgccg cgtgatgctg atgcgctgca gcgtcttgat gggcgacttg gcctggcagg
     3301 cgctggcgcg aaacgacgtg aagtcatgct cgcccagcag gtgttgcacg gccgcctgca
     3361 tggcctcttc gtccagcgca tggaacaccc agccgacgcg gcccgcatcc acgctggggc
     3421 gcacgggtga ctgcagcagc acataggcat aacggcgcgc cacggcgcag gcgcgcgagt
     3481 gaaagctgtc gggtacgggc tgcgcccatt gcacggcgat gtcggcaggc aaaaaggtgt
     3541 tggtgccgcg cacccaggaa aacggcgcgc gctcgagcgc cgtgtcgaag tgcaccacct
     3601 gcatcagccc atgcacgccg gcatcggtac ggccggcgca gatggttgcc accttgtgcg
     3661 tggcaaagcg gcccagggcc gcttcgagat ggtcttgaac ggtattcccg ccgggctggc
     3721 tttgccagcc gctataggcc tggccgttgt agctgacgcc cagggccacc ctcatgccag
     3781 gccccctggc gcgaacggtg cggcgcggcg gcaggcgccg tccctgcgca tggcatgcgg
     3841 catcagccca gctcggccag caggcgctgg gcgcgggcct tgagttcgcc ggtcgattcg
     3901 gcgatgactt cctcgaccag ggtgcgggca ccctcgctgt cgccaatggt gttgaattct
     3961 tcggccagtg ccagcttggt ggtcagcgga tcgtcgggca ggggctccgc agcgccggca
     4021 tccgcttgcg cagcagcagt gggggcagcg ggcaggctca ggtcaaggtt cagatccccc
     4081 aggtcgaatt ccagtgccgc aggggccgag tccttggcat cgctggccgc cagcggggtg
     4141 gctgccgagt cggccagcag caggttgtcg gtgtcaaagt ccaggggcgc gggctcggtc
     4201 gtcagggatg cgaccgcggt gggctcggcg gaacgtggag cggcttcggg ccctgcatcc
     4261 ggcagctcca ggtcgatggc gtccagcgca gcgggcagat cttcgctgac cgcgggcgtg
     4321 gccgctgcgc cttgcgttgc agccagctcg ctcgcggcag cggccatggc gaaggcgctg
     4381 ggcgcgggtg ccggcgctgg tggcgcatcg tgcaggtcga gatccagatc caggtcgaga
     4441 tccaggtcgg gcaacacgga gtcggggccg gtgctggcgt tggtcggcgc gcccacgccc
     4501 acggtgcttg ggaagttgct caggggggcg atgtcgccct cgccgtcagg gcctgcggcg
     4561 gggcggccac cgggttggta cagggcgttg tccgcgtcca gcgtgcgacc caggtctgcg
     4621 acgcgcgacc agtcggggcc ttgcccgtcg gtcagccgga agacatcgct ggcggccgac
     4681 tccagcgcct tgcgatcctg gcgcttggca taaatctcgg ccagcttgac gggtatggcg
     4741 acgcgctccg ggttgtggcg catggcctcc ttgaggattt cttcggcctg caggtcacgg
     4801 ccataggcca gatacacgtc ggcctcggcc acgggatcca cgtctccgcc cacgtcgagc
     4861 tggctgggcg aataggacat cgacgatgcg ccggtggtca ggctgctgtc ggccgtgtcc
     4921 acatgctggc caccactggc tccgaagaat gagtcggggt tgccgctgtc ctggaaggag
     4981 ctgtccagct gcgcctgttg ccgacggctt tgcgccacgc ggtagccacc gtagccgagc
     5041 agcgccagca ccagcgcgag cgcgccaccg gccagcagcg ggtcttcggt gagcgagccc
     5101 aggaagctgg gctcctcata ggtctctggc ggtggtgcgg gcttgggcgc cggggccgct
     5161 gcggctgccg gagcgggtgc cgcttcctgt ggcgtggctt gcggggctgt gggcgcctgc
     5221 tcggctgttg ccggggcttc gggattggcg ttggtctgcg ccggcgcaac cgccccgggc
     5281 agcccggcag gagcagggac ggccaccgcg gactgactgc cagccgcgtt gggcgctgcg
     5341 gcgctggtat ctgctgcagg tgtggcgggc tgtgtggatg cgccactcaa ctggttgagc
     5401 tcggcgatgt tcttggacag ctcggccata cgggccgtgt tctggtcggc ctgcttgtcg
     5461 cgggccatct gctcgtcggc cttgcgggct tgcacgccgc ccttcgacag agtcagcttg
     5521 tcggggctgg cagctgcggg cttggcctct tgcacctgcg cctgcaccga accgctggct
     5581 gaacgctcgg ccgcggccac ctgtgcggtg ggagcggcgc cggccagccg gcggcggaac
     5641 tcgttgaagt cgcggctttg cgctgcgacg atctggcgcg cctcgctggc cggggtggcc
     5701 tgggcggcgg actcgctggg catctgcagc accgcgcccg agcgcagccg gttcacgttg
     5761 ccgtcgacga aggctttggg gttggcctga gccatggcaa ccagcatctg atcgagcgag
     5821 acgctggccg gcctatgggc attggcaatg cgtccggccg tatcgcccgg gcgcaccgtc
     5881 acggtgtcgt cgctgcctgc gccggcctct gtcgcggcag gagcacggct gggggccggc
     5941 gccacccgtg gagtgggccg cggggcggct gctgccggtg ccgccgccgg gggcgcggcg
     6001 ggccgcgccg aggatgtacg ggggggcgtc ggcgcgcttt cgctgcgtgg cgcggccagc
     6061 tgtggtgcca ctgtcacttc ggcgggtttg gcggcctgtg ccggtgggtc gaacagcatg
     6121 gtgtagctgc gcacgatatg gcccgagctc caggtggcat ccagcaccag atcgacaaac
     6181 ggctcgttga tggggcggct gctgctcagg cgcagcacca tggagccgtc ggcacgtcga
     6241 tgcacctgca cctgcacgtt gcccaccgtg ggcgagtatt ccagcccctg gctgcggaac
     6301 gcggccgcgt tggcggtact cacgcgcagc gactcggcct ccgcagccgt gatctggggt
     6361 aattcgatct cggcacgcag cggttcgccc aatgccgact gcacattcac gcgccccagg
     6421 gtcagggcgt tggcatcggt gccgagcagt ccggccgaca gaatggccgc tgtggccagt
     6481 gcagaaaatt tccagcgatg catgtgtgcg acaagtgaat gttgtggtgc ggtcgtgcgg
     6541 agcgttgttg ttcgagtcag gcctagagcc gctccgagac gtgaaaatat gtaaaaagca
     6601 ccatagcatc aatgccttgc actgacaagc cgaggccatg cgccggcatt ccctaccctg
     6661 ttggcaggag ccaaaagttg tctttcatgt tggtaacagg caacgatggg gccgaacagg
     6721 gtagggttgg aggcggatga taaacagcgc ccggccaggg gcgccgttgc cctgcttcag
     6781 gcgctcagca ggatgcgcag catgcggcgc agcggctcgg ccgcgcccca cagcagctgg
     6841 tcgccgatga cgaaggcgga cacgtattca ggccccatgt tgagcttgcg cacgcggccc
     6901 acgccgacct ccaggccgcc ggtgatggcc gccggagtga gttccttgac ggtgatggcg
     6961 cgctcgttgg gcacgaactt cacccagggg ttgccgtcct tgatcaaggc ctcgatctgg
     7021 gccagcggaa tgtccttctt gagcttgagc gtgagcgcca gcgagtggca gcgcatggcg
     7081 ccaatgcgca cgcacaggcc gtcgacgggg atggtggcgg ccgtgcccag gatcttgttc
     7141 acctcggcct ggcccttcca ctcttccttg gactggccgt tgtccagctg cgcgtcgatc
     7201 cacgggatca ggccgccggc cagcggcgcg ccaaagtgct cggtgggcac gtcctcgcgg
     7261 atggtgctgg ccaccttgcg gtcgatctcc aggatggcgg aggcgggcgt ggccagctca
     7321 tcggcgacgg cgccatgcac gacgcccatg cccttcaaga gctcgcgcat gtggttggcg
     7381 ccgccgcccg aggccgcctg gtaggtcatg gaagacaccc agtccaccag gccggccttg
     7441 aacagcccgg ccaggcccat cagcaggatg gagttggtgc agttgccgcc aatccagttc
     7501 ttgccgccgg cggcaagctt ttgccggatc aggccgtcgt tcaccgggtc gagcacgatg
     7561 acggcgtcgc cctccatgcg cagggcgctg gcggcgtcga tccagtggcc ctgccagccg
     7621 gcggcgcgca gcttggggaa gacttccttg gtgtagtcgc caccctggca ggtgatgatg
     7681 atgtcgcact ttgcgagctc ggcgacgtcg ttcgcgtctt tcagctcggt atgtgtcctg
     7741 gccatggcgg gcgccttgcc gccggcgttg gaggtggaga agaacaccgg ctcgatgagg
     7801 tcgaagtcgc cctcggcctg catgcgatcc atcaacaccg agccgaccat gccgcgccag
     7861 ccgaccagac ctaccaattg cttgctcatt tcaaacgccc tttcaagtta aaaacagatc
     7921 acacgcgacc ggtggctgct tttctggccc cggctcgtct ggccagggag ggcgcacgac
     7981 gataccggag ctggatcagc ccttaatggt cgtggttttg gtggtgcttg ctcgcgcact
     8041 atggccggcc agggcggcgg gcttgttctt cagggggaag ggttgcgcgg caaacatgac
     8101 aaaacattgt aggggatgcg atggcgggca ccgccccctg attgttaacg atgcgtcaac
     8161 gcaacgcgtt gaccacggcg tcgcccatct gcaccgtgcc gactttggtc gtgccgtcgc
     8221 tgtagatgtc gggcgtgcgc aggccttgcg ccagcacctt tttcacggcg gtctcgatgc
     8281 ggtcggcggc ctcgggctgg ttcagggaga agcgcagcat catggccgcg gacaggatgg
     8341 tggccagcgg gttggccacg cccttgccgg cgatgtccgg cgcgctgccg tggctgggct
     8401 cgtacaggcc ctgctttttg tcattgagcg atgccgaggg cagcatgccg atggagcccg
     8461 tcagcatcga ggcttcgtcc gagaggatgt cgccaaacat gttgccggtg acgatcacgt
     8521 cgaaggcctt gggcgccttg accagctgca tggcagcgtt gtccacgtac atgtggtcca
     8581 gcgccacgtc cggatagtct ttatggactt cggtgaccac gtccttccac agctggaagg
     8641 tctccagcac gttggccttg tccacgctgg tgacgcgctt gctcctcttg cgtgcggcct
     8701 cgaaggccac gcgggcgatg cgctcgatct ccgggcggct gtagcgcatg gtgtcaaagg
     8761 cttcttctgc gccggggaag tggccgtcta ccgccgtgcg gcggccgcgc ggctggccga
     8821 agtagatgtc gcccgtcagc tcgcggatga tgaggatgtc caggcccgcg accagctcgg
     8881 gcttcaggct gctggcgtgc gtcagttgtt cgtagcagat ggcagggcgg aagttggcga
     8941 acaggccgag cgccttgcgc aggcccagga tggcctgctc ggggcgcagc gggcggtcca
     9001 gcttgtcgta cttccagtcg cccacggcgc cgaacagaat ggcgtcggcg cttttggcca
     9061 gggccagggt ggactcgggc agggggtgac ctgcggcctc gtaggcggcg ccgccaacgg
     9121 gggcggtttc catctcgaac ttcaagtcca gggccttgag caccttgacg gcctcggcga
     9181 cgatttcggt gcctatgccg tcaccaggga gaactgcgat tttcatgtcg atgaactatt
     9241 aaattgatag ctattggcgc ttgttggata agcgccagag gcttgttttg cttaaaattt
     9301 caacccgcca tggtgtgcgc cagccagggc ttggtggcca ggcgctcggc ctcgaatgcg
     9361 gcgatcttgt ccttgtggcg cagggtcagg ccgatgtcgt cgaagccgtt gagcaggcag
     9421 tacttgcgaa acggcagcac gtcgaaggct atttcttcac cctgcgggcg gatgacgacc
     9481 tggcgctcca ggtcgatctt cagctcatag ccggggaagg ccgccacctc atcgaacagc
     9541 tgggccacca ccgactcggg caggacgatg ggcagcaggc cgttcttgaa gcagttgttg
     9601 aagaagatgt cggcaaagct gggcgcgatg atggcgcgaa agccgtactg gtccagcgcc
     9661 cacggggcgt gctcgcggct ggagccgcag ccgaagttct tgcgcgcgat caggatgctg
     9721 gcacccttgt accgtggctg gttcagcacg aagtcgggat tgggcttgcg cgctgattcg
     9781 ggcacgccgg gttcgcccgg ctggtccagg tagcgccatt cgtcgaacag attcgggccg
     9841 aagccggtct tcttgatgga tttcaggaac tgcttgggga tgatggcgtc ggtgtcgacg
     9901 ttctcgcggt ccatgggggc gacgaggccc tggtgcacgg tgaatttctg catggcggtt
     9961 gggtagtgag agggttgtca gcgcgcggcg cgctctatgg cgccgccggc actttgcacg
    10021 tcctggccaa tgcccttgat ggtgttgcag gccgccaggg tcagggccag gccgatcagg
    10081 atgatggaga aagagggctt catgggggcg ctcgcttcgg tcgggttcag gcaaatttgc
    10141 ggatatcgac gaaatgaccg tggatggccg cggcagccgc catggccggg ctgaccaggt
    10201 gggtgcgccc gccggcgccc tggcggccct cgaagttgcg gttgctggtg gacgcgcagc
    10261 gctcgcccgg ttccagccgg tcggcgttca tggccaggca catgctgcag ccgggctcgc
    10321 gccattcgaa gccagcggcg gtgaagatct tgtccagccc ctcggcctcg gcctgcgcct
    10381 tcaccaggcc cgagcccggc accaccatgg ccagcttgat gttcttggcc accttctggc
    10441 ccagcttttt caccaaggcg gcggcctcgc gcatgtcctc gatgcggctg ttggtgcaag
    10501 agccgatgaa gaccttgtcc acgaagatgt cctgcagcgg cttgccgggt gtgagcgcca
    10561 tataggccag ggcgcgctcc atggcgccgc gtttgttggc gtccttttcc ttgtccgggt
    10621 cgggcacgcg ggcatccacg cccagcacca tctcggggct ggtgccccag gtgacctggg
    10681 gcacgatctc ggcgccgttc agttccacca cggcgtcaaa ttgcgcgtcg gcatcggagt
    10741 gcagcgtctg ccaatactgc acggcctggt cccattccac gcccgtgggg gccagcggcc
    10801 ggcccttgac gtaggcgatg gtcttctcgt ccacggccac caggcccgcg cgcgcgccgc
    10861 cctcgatggc catgttgcac accgtcatgc ggccttccat ggacaggtcg cgtatcgcct
    10921 ggccggcaaa ttcaatcgtg tagcccgtgc cgccggccgt gcctatcttg ccgatgatgg
    10981 ccagcacgat gtccttggcg gtgatgcccg gggccacatg gccgttcacg cgcaccagca
    11041 tgttcttcgc cttcttggcc agcagggtct gcgtggccat gacatgctcg acttccgacg
    11101 tgccaatgcc atgcgccagc gcgccaaagg cgccgtgggt gctggtgtgg ctgtcgccac
    11161 agaccaccgt catgccgggc agcgtggcgc cgttctccgg gccgatgacg tggactatgc
    11221 cctggcgctt gtgcaggaag gggaagaagg cggccgcgcc cgatgccgca atgtttttat
    11281 cgagcgtggt gatctgctcc ttgctgatcg ggtcggcaat gccgtcgtag ccgcgctccc
    11341 agccggtggt gggcgtgttg tggtcggcgg tggcgacgat ggagctggtg cgccagacct
    11401 tgcggcccgc ctcgcgcagg ccctcgaagg cctgcgggct ggtgacctcg tgcaccaggt
    11461 ggcggtcgat gtagagcacg gccgtgccgt cctcttcggt gtggacgacg tgctcgtcga
    11521 agatcttgtc gtacagggtg cgtcccatgg cggtctcgtt tcgtgggggt cttgggttca
    11581 gggggatggt gaaattttag gcgccaggtg ctccaccagc aggcgcgcgg tgaccggcag
    11641 ggcgtcgaag tcgcgcgcca ccaggcgcag ctcgcgctcg gcccagggtt catccaggcg
    11701 cacggcggcc aggcgcccca ggccctgcat cagggcaaag gcgcggtcgg gcagcaggcc
    11761 cacgcccagg ccgttgtcga tcatgcggca catggtgtcc aggctggtca cctgtatgcg
    11821 ctggcgcagc ggccgcccgg cctgggcggc ggcggcgcgc atcgccaggc tgatggaact
    11881 gccggcatgc aggccgacga tgtcccagtc cagcacctca tcaaatttga tagcttctcg
    11941 cgcagacagg gcatgcgttt gcggcatgac cagcaccaat ctgtcactgc ggtaggggcg
    12001 gctttgcagt tcggcggcgc cgttgccctg gctggcgttg cccaggctgc agatgcccag
    12061 gtcggccgcg ccctcctgca cggcctgcag cacctcgggc gagagatgct cctgcaggtc
    12121 gatcttgatc tggctgtggg cgcgcgcgaa atgccccagg tcctcgggca gaaactgcac
    12181 gatggccgag atgttggcgt gcatgcgcac atggccgcgc acgccctcgg cgtactccga
    12241 cagctcgccc tgcatgcgtt ccagcccgaa gagcacggtg cgcgcgtggt gcagcaggct
    12301 ctcgccggcg ggggtgagcg tcacgccgcg gctgtggcgg tagagcaagg ccgtgtccac
    12361 ggccgcctcc agatccgaca ggcgtttgct cacggccgag gcggcgatga actcgcgctc
    12421 ggccgcgcgg ccaatgctgc ccagctcgca cacggcgacg aacagttgca gcgaggtcag
    12481 gtcgatacgg cgcgcaaaac tgcgctcgga gcttttcatg ggggatgggt tggcttctcg
    12541 tttggagaag aatttgtgta ttttccctca atattcacgt cttgccatcg tcaaatgcga
    12601 tgacttgtgc atcgctgtgc gcatgggctc cgaaattact cctgaatcaa tagctgtttg
    12661 cgctttattg aagggcgtta gagccgtatt tgatcaatat ttcaaaaggc cacggtatcc
    12721 ccatgctcgg gtacttcggc cggccagagc agctcatgct ccacgcgctg gcgcagctgg
    12781 tcggccgcag gcagctcgcc atgcaccaca tagatgcggc gcggcgcggc gggcagggcg
    12841 cgcagccagt ccagcagctg gttgccgtcg gcatgggccg aggccgtggc cagctgcacc
    12901 acctcggcac ggatctgcac ctcgcggccc aggatgcgga tggtcttttc ccccgccgcg
    12961 atgcgcgcgc cgcgcgtgcc gccggcctgg tggccagtga ggatgatcat gttgcggtgg
    13021 tcgcccgcat gcaaggccag gtggtgcagc acgcgcccgc cggtggccat gccgctggcc
    13081 gagaggatga cgcgcggccc gtgcaggcgc gccagggcct tggactggtc cacgctgtgc
    13141 accagcgtgg tgccgcgcgc catggcctgc aggtcggcgc ggctcaggca atgctcgcct
    13201 acatggtttt ggaacagctc ggtggcgctt accgccatgg ggctgtccag gtacacgggc
    13261 aggccggtgg gcagctcgcc acgggccttc aaccgggcga tggcgtgcag caccgtctgc
    13321 gcccgcccca cggcaaacac cggcaccacg gccacgccgc cgcgcgcggc caggcgcgcc
    13381 agcggtggcc ccagttcatc gagcaccgag ccgctggggt ggccgcggtc gccataggtg
    13441 gattcaatca gcaccgtgtc ggccgcgggc ggcgcgtcgg gcgcgcacat cagcaggtcg
    13501 tccggccggc ccaggtcgcc cgagaacagg atgcgccgcc cgcccacctc cagcagcacg
    13561 ctggccgcgc ccaggatatg gccggcgcgc gtgaaggtgg cacgccagcc gggcaggggc
    13621 tcgaactgct ggtgcacggg ctgcacctgc agcagagcca ggctgtgctg cgcgtccagc
    13681 cgcgtgtaca gcggcagcgc cggctcgtgc ttgctcagtc ggtggcggtt gaggtaggcg
    13741 gcctcttcct cctgcaggtg gccgctgtcg ggcagcagga tggcgcacag cgcacgcgtg
    13801 ccgctggtac agaacaccgg cccgccaaag ccgtcccgcg ccagcagcgg caggtagccc
    13861 gaatggtcca gatgggcgtg ggtcaggatg acggcatcga tctcctgcgg cgccaccggc
    13921 aatggccgcc agttgcgctg gcgcaagagc ttgtagccct ggaacagccc gcaatcgacc
    13981 atcagcacct ggccgccgtg acgcaccagg tatttggagc cggtgacggt gccggcaccg
    14041 ccgaggaagg tgaggctgac tggcatggcg ccctccgtgc tgctatgcct gccagggtag
    14101 caggcttggg cggtcatggg gcagtctccc gctgccgccc gcactcaagc gcggcggtgc
    14161 gacctgctgg ctcaatgcgc gggcttgacg gcgcgaatga aggcgctgag gaatttgccg
    14221 tccatctcgt cggaaaccac cgaggtgtcg agcccgttgg cgctcagaaa ttcgcgcgcg
    14281 tcctgcgccg tgtagatgcg cgtgggctcc acgccgatgt cggaaaagcc ggcggcggcc
    14341 agcttgtggc ggtagtcgtc ctcgtccagc gcgccggcca cgcagcccac ccaggccatg
    14401 gcgctggcgc gaatctcggg gcggatggcg ccgcgcgtga ccacgtcgga cacggcaaag
    14461 cgcccgccgg gcttgagcac gcgaaacgcc tcggccagca cgcggtcctt gtcggccgag
    14521 aggttgatga cgcagttgga gatgatcacg tccaccgagg cgtcgggtag cgggatgtgc
    14581 tcgatctcgc ccttcaagaa ttccacgttg gtggcgcccg cctggcgctg gttgtcgcgc
    14641 gccagcgcca gcatctcgtc ggtcatatcc aggccgtagg ctttgcccgt ggggccgacg
    14701 cgcttggccg acagcagcac gtcgatgccg ccgcccgagc ccagatccag caccacctcg
    14761 ccggcgcgca gctcggccag cgccgtcggg ttgccgcagc ccagcgaggc cagcacggcg
    14821 gcctcgggca ggccgccggt ctgcaccgcg tcgtacaggt cgcgcgtgat ggggtcggtg
    14881 ccggccagcg ggccggtggc gcagcaggag ttggtgggcg cggccacggc gccgcggcag
    14941 gaggaggcag cgccgccctg cgtgacgcgg cgcgccgcct caccgtactt ttccttgacg
    15001 atttgctgga tgtcctgggt ttgtgtcatc gcggttctcc tatgggggta catgtctggg
    15061 ccacggacgg gctcgctttc tcgccggcaa accagcgccg ctgccaacgc agcgccaggg
    15121 ccaccagcgc gatcatcacc ggcacctcga ccagcgggcc gatgacggcg gcaaacgccg
    15181 cgccggaatg gatgccgtag acggccaccg ccacggcaat ggccagctcg aagttgttcg
    15241 acgcggcggt gaacgacagc gtcacgcagc gcgggtagtc ggcgccggcg cgccggccca
    15301 tggcgaagga cagcacgaac atcagcacga agtagatgag cagcggcagc gcgatgcgca
    15361 gcacatcgag cggcagggcc agcaccgtgc gcgccttgag gctgaacagc acgacgatgg
    15421 tcagcagcaa cgccaccagc gtcagcggcg cgatgcgcgg cacgaaacgg gtgtggtacc
    15481 agtctttgct gacaaagcgc agcagcaccc agcgcgtgag caggccggcg atgcagggaa
    15541 tgcccaggta gatgaacacg ctttcggcga tctggcccat ggagatggcg acgaccgagc
    15601 cgcccaggcc aaacagcggc ggcagcacgg tcaaaaacag ccaggcatag acgctgtaga
    15661 acagcacctg gaagaaggcg ttgaacgcga ccagcgcagc ggcgtattcg gtcgagccgc
    15721 gcgccaggtc gttccagacg atgaccatgg cgatgcagcg cgccagcccg atgaggatca
    15781 gcccggccat gtattcgggc tggtcgggaa gaaagacgac ggccagcacg aacatcagca
    15841 ccgggcctat gacccagttt tgcagcagcg acagccacag cacgcgcgtg tcgcgccaca
    15901 cggcgggcag gtcttcgtag cgcaccttgg caaacggcgg gtacatcatc agcaccagac
    15961 cgatggcgat ggggatgttg gtactgccga cctggtagcg gccgatgatg gcctcgatgt
    16021 ccggcaccag ccagcccagg gccaggccca gcgccatggc ggcgaagatc cacagcgtga
    16081 gatagcggtc gaggaaagag agtttgcggg tgagctggct catggcgggt tggcggcggg
    16141 tcaaaggcgg gagagttcgg tgtgcagggc ggctgcgccc cgggacgcca gcggcagcgc
    16201 cagcaggcgt tgcaggcgct cggccagggt ctggcgcgtg tgctcgaagg cggcgcgttg
    16261 gccggccgcg ccgcccggcg cgctggacgg gtcggcgtag ccccagtgca gccgcaccgg
    16321 gttgccgggc cacagcgggc aggcctcctc ggccgcgctg tcgcagacgg tgatgacgat
    16381 gtccatgggc ggcgcatcgg cggcgctgaa ttcgtcccag ctcttgctgc gcaggccctg
    16441 tgtggccacg ccggcggcgg ccagcacctc cagcgcctgc gggttgacgc gcccgctggg
    16501 cgcgctgccg gcgctgtgcg cgcgcacgtc cagcccttgc gcggcggcca ggtggttgag
    16561 catggcctcg gccagcacgc tgcgcgccga gttgtgcgtg cacaggatga gaatgtgggt
    16621 ggtcatgggg tcagcagcag ggtgcggatg gggtggcggc gcaggcgtcg ccctggcagc
    16681 agttctcggt cagaaaggcc agcagcgcgt tcatctgcgc gaagtcggcg cggtagatca
    16741 ggctgcggcc ctggcgctct tgcgtcagca ggccggcctg caccagctcc ttgaggtgaa
    16801 acgacagcgt ggccgccggt atctgctgcg cctcggcgat ctgccccggc gtcaggccgg
    16861 cctggccggc gaccaccagg gcgcggaaca cgcgcagccg gtgcgcttgc gagagggctg
    16921 tcagggcctt gagtacggat tgttcttcca ttattcgata attatggaaa tgatgaggcg
    16981 agtgtatgcg acgcctggtg ccgtggcaag ggggtgtgaa aagaccgggc gaaagatcta
    17041 ttgcagccgc atgtgcagcg tggcgtagcc ggcgtccggc gccacggcgc gggtcggggc
    17101 gtggctgggg ctgagcgtga tggcctgggt gcgcgccagc acttcctcga tcagcacgcg
    17161 cagttccagc cgcgccagcg gcgccccggg gcagacgtgg atgccggcgc cgtagagcag
    17221 gttcagcgcc gggtcgcgct ccaggcggaa ctcgcccggc tggtcgaaca cgcgcgggtc
    17281 gcggttggcg gcgatccagt tgatggccac gcgctcgcct gcgggcagcg ggcagccgcc
    17341 cagcgtgacc gggcgcgtgg tgatgcggcg gttgccgacc agcgggccgt cgatgcgcag
    17401 cacctcgtcg atggccgcgc ccaagagggc gggctgggcg cgcaggcgct gctgccagtc
    17461 ggggtggctg gccagccagt gcagcaagat gcctatggac gcggcaatcg tgccgacctc
    17521 gcccaccgtc cagttgcgca gcacgctggc gagttcgcgc agatccagca ggcgcccgtc
    17581 gacgcgcgtg tgcatcagct cgctgctgat gtcctgcgtg gccggcacgc cctggcgcgc
    17641 ttggcgcacg cgctgcacca ggtcttcaaa ttcctgcgcc acggcggcgg tctgcgcgcg
    17701 ctcgcccgag cgggtggccg cctggctgcg gcgtagccac tgcagcagct cgtcctgcag
    17761 cagcgtcggc cagcccagaa acgccgactg cacgcgcacg gcaaagggca gggcgaactc
    17821 ctgcgtgatc tccaggctgc cgcccagctg cagcgcctct tgcacggtgg cggcggagag
    17881 cgcacggcac accggctcga agcgcgccat gcgttcgggt gcgaagaaat gatcgagcgc
    17941 ctggcggtag cgcgtgtgct gcggctggtc gtagccgttg ggcacggccg gatgctgcga
    18001 cacggcgctg ctgtaggtgg cgtggtcttg cagcacctgc atgacttcgg ggtggccgaa
    18061 caccgtggtg tgctggcttg cgctgtgcgc cagcgggcag cgcgcgcgca gctggtcgaa
    18121 ggcggcgcgc tggtcgcgct gcacctcggg ggcttgcggg tcccaggggg tgggggtgct
    18181 gctcatggcg ttctcctttt acaggcccag gtcgtcggcc aacgtatcca tgcgcgcctt
    18241 gaccgctgcg tccatggcca tggtgcggcc ccattcgcgc tgggtctcgc cgggccattt
    18301 gttggtggcg tccagcccca tcttgccccc taggccgctg acgggggaag caaagtcgag
    18361 gtagtcgatg ggggtgttcg ctatcagcat ggtgtcgcgc accgggtcca tgcgcgtggt
    18421 catggcccaa atcacttctt tccaatcgcg cacgtcgata tcgtcgtcga cgatgacgac
    18481 gaacttggtg tacagaaact ggcgcagatg gctccacacg cccatcatca cgcgccgcgc
    18541 gtggccggcg taggccttct tgatgctgac caccgccatg cgatagctgc aggcctcggg
    18601 cggcaggtag aaatcgacga tctcgggaaa ggcgcgctgc aacagcggca cgaacaattc
    18661 gttgagcgcc aggcccagca tggccggctc gtccggcggc ttgccggtgt aggtgctgtg
    18721 gtagatggcg tcacggcgct gcgtgatgcg ctccacggtg aacaccggga actcggcgca
    18781 ttcgttgtag tagccggtgt ggtcgccgta ggggccctcc agcgcgtgct gccagccgct
    18841 ggggtgctgg gcatcggggt ggatgtggcc ttccaggacg atctcggcct gggccggcac
    18901 ctggagcggc acgcccagcg cgtcggccac ctcggtgcgc gcaccgcgca gcaggccggc
    18961 gaactggtat tccgacagcg tgtcgggcac gggcgtgacg gcgcccagaa tggtggccgg
    19021 gtcggcgcca atcgccacgg ccacggggta gggttggccg gggtgttgcc ggcaatgctc
    19081 ggcaaagtcg agcgcgccgc cgcggtgcga gagccagcgc atgatgagct ggtttttgcc
    19141 gatgacctgc tggcggtaga tgcccaggtt ttgccggcgc ttgtgcggcc cgcgcgtgat
    19201 cgtcaggccc caggtgacca gcggcgccac gtcgccgggc cagcagtgct gtatcggcag
    19261 cctggacaga tcgacctcgt cgccctcgac caccagctcc tggcagggcg ggcggcgcac
    19321 gttggacggg gccatgctcc acagcgtctt gaccagctcg cgccggcccc acaggtcttt
    19381 catgccgcgc ggcggctcgg gctctttcaa ggacgccagc acctcgccaa aggcgcggat
    19441 ctcaggcagc cccgctacgc ccagcgcccg cgccacgcgc gccggcgtgc caaacaggtt
    19501 ggccagcacc ggcatggcgt ggcccgtggg gttttcgaat agcagcgccg ggccgcccgc
    19561 gcgcagcacg cggtcggcaa gggccgtcat ctccaggtgt ggcgagaccg gggcggcaat
    19621 gcggcgcagc tcgcccgcgg cctccagctg ggccatgaag tcgcgcaggt cgcggtagtg
    19681 ggtttgcgtc aaaacgggct ccagcgctta tgtatcaagc gcaagcagct atcaattcag
    19741 gactgtccat gcaggccctg ccagcgcggc gccagtgcat gctccagacc cagcaggtcc
    19801 agcgaccgcg ccacgctgtg ctgcacgatg tcctcgacgg tctgcggccg ctggtagaag
    19861 gcgggcacgg gcgggcagat gatgccgccc atctcggtca ccgtggtcat gttgcgcagg
    19921 tgcaccaggt gcagcggggt ctcgcgcgcc agcagcacca ggcggcggcg ctctttcagg
    19981 atcacgtcgg cggcgcgcgt cagcaggttg tccgccaggc catgggcgat ggccgccagc
    20041 gtgcgcatgg agcagggcgc caccaccatg gcctgcgcgc cgaaggagcc gctggcaatg
    20101 ctggcgccaa tgctggcgtt gtcatgcgcc tggtgcccca gggccagcac ctgctcgcgc
    20161 gacagggcgg tctcgtgctg gatgttgagc cagccggcat cggagacgac caggtgcgtc
    20221 tgcacccccg gcatggcgcg cagcgcctgc agcagggcca cgccatagac ggcgccgctg
    20281 gcccccgata tgccgacgat gatgcggcgt ggctcagcca tgcgcaggca ccagcggggc
    20341 tgcagccagg tcggcctgca catgtgccag cacctcgtcg gcctgggccg ggtcgaagtc
    20401 ctggtggttg cgcttcttca cgccgtactt gtgcagctgg tagtggcgtg ccggcgtgac
    20461 gctgtggcgc gccagtgaat tgcgcacgca ttccagcggg cagccgtcca gtgcgatgac
    20521 ggggcggccg ctcttggcga gctgcaccag cttgggcacg tcgccgccga cgccggcaat
    20581 gcatgacatc tcggccaggc cgcgcctgtc catttgcacc gccacatggt tggccagctg
    20641 cgccgcgctg gagcagcccg agcaggaata gaccagcggc ggcaggggcg caggggccgg
    20701 gtcggtcatg gccgtcccct cagaaagcgc ggcagcggcg gcagctggcg cagcagctgc
    20761 ggcgccttgc gctcgaggac tgcgcgcggc gtccactcgc gcgggtcgag cacgggcagc
    20821 tcgagggcgt ccagcgcgtt tttgaccacc aggccgagct cggtgtcgcc ctccatcacc
    20881 aggcggcggt tgaagaacag cgtgtccggg tcttgccggc gctgcgccag cagcaggaag
    20941 tcctgggcgt tggcgctgat ggccagatcg gtttcggcct gcggcttgca gggcacgaag
    21001 cgctggccgt tccatgagaa gtcgaacgcc acgcgcgcat cgcgcacgcg aatcgccaga
    21061 cgcttggcgc gcaggatgtc tttcacgtcc tgcggcagct gtttggccag ggcgaagttg
    21121 atggccgtga ccagcagcat ggagcccggg taggccggca gacgcgagag cagcgcgccc
    21181 acgggggcgg gcagggtgaa gggggcgggc ggggtggtgg tcatggggat gcctctcagt
    21241 ttttcaggcc agggcgggtt cgatcaggtc caggcccggg cggccatgcc agtagccgtt
    21301 gcaggacgcc tcgggcatga aggggcgcat ggcttgcagc gcggccgggt tgggctgcgc
    21361 ggcgcggcgc gcggcgtcga acgcggcaat gatcttgtcg gtgtgctgcg actgtggcga
    21421 gatgcgcagg atgtccacgc cggcggcgcg cagcgcgggc atgtcgccca ccaggttgtg
    21481 caccttggcc gactgcgtct gtatgccgtt gatgaccaga aaggattctt tctcgcgcgt
    21541 ctgcaagagc tggccgtccg ggtgctcgat gcagcgaaaa ccgcagtcgt ccttgggcag
    21601 gttgtagtgc cgcgccgtga agcagcgtgc cgagaaggcc agcggcatgc ggccgtaggc
    21661 gaagacctcg gtctccagtc ctgcgggccg ggtctgcagc agcacggcca ggtcgctgtg
    21721 cttcatctcc agcggcatca cccagcgact ggcgcccagg ccggccatcc aggccaggga
    21781 atcggcattg aagaggttga gctgcggccc ggccacgaag gggcgcttgc ccgccagctt
    21841 gtgcaccgcg cccatgtcgt tcgcctcgat gaggaattct tcatcgcgca cgatcttgtg
    21901 catgtcggcc acgtcgctgg tggattcgag cagcaccatg gtggacatca cggcctgctt
    21961 gccggcatca cgcagcatgc gggcaatgtc cagccagtcg ctttggcgca gctcgcggcg
    22021 gcgcgagcac acggcctcgc ccaggtagac gatgtccacc ggcgactcgg ccatttgttg
    22081 gtagaactcg aagacggtgt cgcggtgcca gtagtactga agggggccta gggctagttt
    22141 catcatggtg gtttggttgt tctctccctc tcccccctgg gggagagggc aggggtgagg
    22201 gggcgaggcg cggcggtggt cttgcctggc accctcaccc caaccctctc ccgcgtgcgg
    22261 gagagggagt tggcgaggca atagggtgtc acttccatgg ccggtgatat gcccccagcg
    22321 tgtgctgctg gccctcggcc acctggtcca ggctggccat ccaggcggtc ttgggggcgt
    22381 acaaatggcc ctgcgccatg caatggtcga tggcctcgcg ccagaccttg gtgacctggg
    22441 ccacgtaggc ggggctgcgc tggcggccct cgatcttgaa ggcgcgcacg cccatcttca
    22501 ccagctgggg caagagctcc agggtgttga ggctggtggg ctcttccagc gcgtagtagt
    22561 tgctctcgtc acccacgtca aagcggccct tgcacagcgt cgggtagccg gcgttctcgc
    22621 cctgggcgta gcggtcgatg agcacgccgt tcaggcgcga ctccaggcct ctgggcgtct
    22681 cctgccagcg cacggacttc gggggcgagc acacgccatg ggtgttgggt gattcgcccg
    22741 tggcgtagga cgacagggcg cagcgcccct ccaccatcac gcacaggctg ccaaagccaa
    22801 acacctcgat ctccacgtcg gtgcgctgcg tcacctgctt gacctgctcc agcgacagca
    22861 cgcgcggcag cacggcgcgc tggatgttga actgctgctg gtacaggttg atggcgtcgg
    22921 cattcgtggc cgagccctgc acggacaggt gcaggcgcaa gtcggggtgg tgctcgttgg
    22981 cgtagcgcat caggcccggg tcggccagga tcaccgcgtc tatgccccag gcgctagcgc
    23041 gatccagggc cgagcgccag ggcgtggagt tgcttgcctg tgggtaggtg ttgagtgcca
    23101 tcagcacctt gcggccgcgc tggtgggcgt aggcaatgcc ggtctggatg gcggcctcgt
    23161 caaagttcag gccggcaaag ttgcgcgcat tggtggcgtc gcgcaggccc aggtagacgc
    23221 agtcggcgcc gtggtcaacg gcagttttca gggccggcag gctgccggcg gggcagacca
    23281 gctcgggggc tgtgcgggcg gggctggtgc cggtctctat gggggtggag ggactgagga
    23341 tggcgttcac tgtgggtctc gatgcaatct gtgtcgggag gcttggctcc tgaggccgca
    23401 caccatagga cgatgcgaaa gaccacagcc tgatttttgt caagacaggg ggtatttccg
    23461 aggaaaaccc tcgggcatgg gcgggcagca ggttttttcg tgaactgttg gaaaagaccg
    23521 acaccatggt ttttgtcgcg gggctacctt cacccaccca acagatacgt ggatagccca
    23581 ccatgcccaa gaccctgatc gaaccctttc gcatcaagag cgtagagccc atacgcatga
    23641 ctaccgccgc cgagcgtgcg cgcctgcttg aagaggccag gctgaatgtc ttcaagttgc
    23701 gcgccgagga cgtgctcatc gactggctca ccgactcggg caccggcgcc atgtcggcgc
    23761 gccagtgggg cgccatcatg gagggcgacg agagctacgc cggcgcgcgc agcttcttta
    23821 ggctggagaa ggtgatccag gacatcaccg gcatgcagta tttcgtgccc acgcatcagg
    23881 gccgcgcggc cgagaaggtg ttgttcacgg cggtgtgcaa gcagggcgac ctggtgccca
    23941 acaactgcca ctttgacacc acgcgcggca acctggagta cctgggcgtc gaggcgctgg
    24001 acctggtcga ggccgtgggc ctgcagccgg ccaccatcgc ccccttcaag ggcaacatcg
    24061 acctggcgcg cgccgaggag gtcttgaaaa aggagggcca gcgcatcccg ttcggcatgc
    24121 tcaccgtgac caacaacacc ggcggcggtc agccggtttc catggccaac atccgcgcct
    24181 atgcgcagct gctcaaaaaa tacggcaagc ccttcatcat ggacgtgtgc cgcttcgccg
    24241 agaacgccat gttcatcaag atgcgcgagc cgggctacga aaacacctcc atcaaggcca
    24301 tcgcgcaaga gatgttcagc tacgccgacg gcgccaccat gagcgccaag aaggacggca
    24361 tggtcaacat cggcggcttc atcgtgctgc gctctgacga atggctggac gaggtgagga
    24421 gcgacctgat catgatggaa ggctttccca cctacggcgg catggccggg cgcgacctgg
    24481 aggccctggc cgtgggcctt gaggaggggc tggatgagga ctacctgcgc tatcgcctgc
    24541 gaacggccga gtacctgggc gagaagctgg acgctgcggg cgtgggcttt gtgcgtccca
    24601 cgggcgggca cgcggtctac atcgacgcca agagcgtgct gccgaatatg tcggttgaac
    24661 actacccggc ctgggcgctg tgcaacgcgc tgtacctgga gggcggcata cgcggcgtgg
    24721 agataggctc ggtcatgttc ggcaagcgcc tgcaggatgg cagcgagacc taccacagca
    24781 tggaactggt gcgcctggcc ttcccgcgcc gcatgtacac gcaaagccat ttcgactacg
    24841 cggccgaggt gatcgccgag gtcaagcaaa aggccgccag cattcgcggc gtgaagatca
    24901 tcaagcagcc gaagttcctg cgccacttca cctgcgagtg cgcctgggtg tgaggcaggt
    24961 gcggcgggtt aaccagtgcg ccgcatggcg ccgttgccgc cccgctcgcg cagcgcctca
    25021 tatagctgcg ccggccccag caaaatggac ttgagcccgg cgcgcacgcg ctcgctgtcc
    25081 agcgactgtt tgctcatcgt tgtgtacttg gtgaacccgt cgatgatggc gtcaatcaac
    25141 gcgcgatcca ggtcgggcga gttgtcgaac tgggccttgc tgttgttgct ggcctgctgc
    25201 accaggatgt ccgattcgag caacttgccc ttgacgtggt tcacatatac cagccggtcg
    25261 tcgtcggaga gatccccgtc gaacaggtcg ttcaccttgg cgacgatctc cgacagcagc
    25321 agcttttgct tgtcctgcac cgcgccggcg cctgtttcgg tcaggggctg cagcttgtac
    25381 tcgctgacca tatcgtgcag cgccaggctg cgtgcgccct ggtctttcag ccgatggtgg
    25441 gtcagcacca ccttggagag gtccaccccc tcccgctcgc ggccaaagtc caagagcggc
    25501 agcaggcgct tgaagaagat ggcgcgcttc tcgatagccg tgttgccgta gtcgaacacc
    25561 tggcccagaa aggcatacac gcgcacaaag ctgcccaggt cgcgcttgaa cagttgcagc
    25621 gcctcttgtt cgtccttggc ggcctgcgtg cccgggccgt ctgaatccgc ctgctgtgcc
    25681 tgttgcgctg cacggtagcg cctgagcagc cggtcggcca ccggctcgat ggcggcggcc
    25741 agcatcgcct gcgtggccct ggggttgagc tcggccagca ccacgcgctc gacctcgaag
    25801 tcgtcgtaat gccccgccgc gtcgagcttg gcgcgcaggt cgtagaccag gtgcgggtcg
    25861 gtcaccccgg ccagctcggc ggtgtcgtaa taggtcttga acgcggccag cacctcgtcg
    25921 gggtcgttga caaagtccag gatgaacgtg gtgtccttca ggccatgggg gccgctgtag
    25981 gcccggttca ggcggctgag cgtctgcacc gcctggatgc cgcacaggcg cttgtccaca
    26041 tacatgccgc acaagagcgg ctggtcaaac ccggtctgaa acttgttggc caccagcagg
    26101 atttggtagt catccgtgga aaacgccttg cgcatgtcgc ggcccttgag cagtgggttg
    26161 agccgcgcgc tgttctcggt cacggattca tccgcctcgt cggggtccgc cacctcgccc
    26221 gagaacgcca ccagcgtgcc cagcgggtat gcggtcaaag cggtactggt ccaccgccgc
    26281 ctgcacgcct tgcgtgaact cggttttcag ctcggccgtg gccaccggaa tgccgttcaa
    26341 gaacagcacc agatcgatga tgtcgtcgtg gttggtacgg cactggcgca ccacgcgcag
    26401 gcggttggcg ccgtagcgcg cctgcaaatc cgggttcatg gccagcgcgg gcttgaactg
    26461 cgcgagcctc aaagggcttt tcagccccag caggtctatg cccacacgca ggacttcgag
    26521 cgtgccgcgc tcgtccagcg cgcggcgcag gcgatcgagc agcatggcgg gggccgccgc
    26581 gccgtggttc ttggccagca cctcccaggc ctgcggctgc gtctgctgca cccaggtcag
    26641 caggtcggcg gggtacaggg cgcgggcggt gtcgtagccg ctggcgtcgc cctcggcgcc
    26701 gggtgcgccg tagagccagc catgctgcgc cagatggttg cagatgtcgg tttcaaacgc
    26761 gatttcttgg tgcagggcca tggcaaaccg tggttcagtg ggtggagtct gcgtcttgct
    26821 ggtccagccg atccagaatc gccagcgcct gcgcggcatc gccactggcg gccagggtgc
    26881 gaaagcgggt ttccgcatca aaggcagcaa tggcctgggt gctgagttct tccatcagct
    26941 tgttgatgct caggccccgg gtgtgggcca ggttcttcag gcgctgcgcg gtgtcgtcgg
    27001 gcaggcggat ggtcagggtg ctcatggctg ctcctccaaa aattgggcgg gggtcaaaat
    27061 gcgcaagcgc ggccaggcca gctcgccccg cgccaggtcg cgcacgttgt gcgtcaccac
    27121 ggcctgggca ttgcccgcca cggccagctc gatcaggtgg ttatcgccct catcgcgcag
    27181 attggggcgc cagccgtaat acacctgcac ccagcggccc gcacgcgcca gggcggccag
    27241 cacctgcaag cgctcttcgg gcgtggtggc gctcgtccag acgtcgcgcc ccagcaggtc
    27301 ttggtactcc tgccacagcg tattgccaaa cagcggcgta gcctgccctt gcaggatgcg
    27361 acgcaacacc tggcgcgacg ccccgccgcc cgagcgcaaa gcggagacaa aaacgttggt
    27421 gtccagaacg atggtgcgca tggctcatgc tagcacgggt gctggcatat ccatcatctg
    27481 gagtatggtc gaatggtgca aaaactggct tgaaatgcct gccagataag ccttggcagc
    27541 tatgaatttc aagaatccgc ccgcgcgcgc tgatcttccc gctgacggtc acctccagca
    27601 gcaccagcag gtcttggggt ttgggctcca tggtgccatt cgctattcac gaatagagaa
    27661 tgcgccttgc gggtcatggc cgccagctca gggaacagag ggtgctgcac gttggcgttg
    27721 tgccgggaac agcaggtgct tggcggccag ctcggccagc accgcggcct tggtggattc
    27781 gagcacgcag tccaggcggc gcagcacggt gaagggcagg atgaccttgc cgtagtcgga
    27841 ctgcttgtag tcgccacgca gcaggtcggc aacggaccag aggaaggcgg agagggtttg
    27901 ctggttcatg gaagctgggg gctgggccgt tggggtgtgg cgaatccgct aaaagacaaa
    27961 ggccataccg ggcggggcat ggcctttgcg aagaagcggc ttgagcgcgc tcagctgaag
    28021 aagctcttca gccggtcggt ccagctatcg gtgctgggcg aatgcttgcc gccgcctttt
    28081 ttcagcgact cgtccagttc cttgaacagc ttgcgctggt gctcggtgag cttgaccggc
    28141 gtctcgacga cgatgtggca gtacaggtcg ccggggtagc tggcgcgcac gcccttgatg
    28201 cccttgccgc gcaggcggaa ctgcttgccg gcctgcgtgc cctcggggat gtcgatggcg
    28261 gccttgccgg ccagcgtggg cacctcgatc tcgccgccca gggccgcggt gatgaagctc
    28321 accggcacct ggcagtgcag gtcgtcgccg tcgcgctcga atatgtcgtg ctttctgacg
    28381 cggatctcga tgtacaggtc gcccggtggg ccgccgttgg tgccgggctc gccgttgccg
    28441 gtcgagcgta tgcgcatgcc gtcgtcgatg ccggcgggga tcttcacctc cagcgtcttt
    28501 tgcttcttga tcttgccctg gccgtggcag ctggtgcagg gctcgggaat gatcttgccg
    28561 gtgccgcggc aatgcgggca ggtctgctgc acgctgaaaa agccctggcg catctgcacc
    28621 gcgccgctgc cctggcaggt gccgcaggtc ttggcgctgg tgccgggttt ggcgccgctg
    28681 ccgtggcagg tgtcgcagga ctcccagctc gggatgcgga tctgcgcgtc cttgccgtgg
    28741 gcggcttctt cgagcgtgac gtccatggcg taggacaggt cgttgccgcg atacacctgg
    28801 cgcccgccgc gcccgccgcg ctgaccgccg aacatgtcgc caaagatgtc gccaaaggct
    28861 tcggcaaagc cgccaaagcc ctcggtgccg cccatgccgc cgcgcatgtt ggggtcaacc
    28921 ccggcgtggc cgtactggtc gtaggcggca cgcttctgcc cgtcggagag catctcgtag
    28981 gcctctttgg cctccttgaa tttctcctcg gcattcttgg ccgcatcacc ctggttgcga
    29041 tccgggtggt gcttcatcgc cagcttgcga taggccttct tgatgtcgtc ctcgctggcg
    29101 ttcttgggaa cgcccaggac ttcgtaatag tctcgtttcg acatggttgg attcgcctgc
    29161 ttggaagagg aacgccgcgc gggctctcct tgaggtggag gccggcgcgg cggtgtgttt
    29221 cagcgcaagc gctgggtgat cacttcttca cttctttcac ttcggcatcg accacgttgt
    29281 cgtcctgcgc tgccgcgccg gcagacgccc ctgcagcggc ttgcgcgcca gcagcttcgg
    29341 cacctgcgcc ctgggctgcc tgggcgtcgg cgtacatctt ctcgccgagc ttctggctgg
    29401 cggtcatcag ggccgtggtc ttttcctcga tggcggcctt gtcctcaccc ttcaaggcgg
    29461 cttccaggtc cttcaccgcg gcttcgatct tctccttctc gccggcgtcg agcttgtcgc
    29521 catgctcggc caggctcttg tgcacggtgt gcaccgcggc ctcgccctgg ttgcgtgcct
    29581 gcaccagttc cagcttcttg tgatcctcgg cggcgttgag ttcggcgtcc ttcaccatct
    29641 gctggatctc ggcctcggac aggcccgagt tggccttgat ggtgatcttg ttttccttgc
    29701 ccgtgccctt gtccttggcg cccacatgca ggatgccgtt ggcgtcgatg tcaaagctca
    29761 cctcgatctg cggcgtgccg cgcgcggacg gcgggatgcc ttccaggttg aattcgccca
    29821 gcagcttgtt ggcgttggcg atctcgcgct cgccctggaa caccttgatg gtcacggccg
    29881 gctggttgtc ctcggcggtg gagaaggtct gggcgaactt cgtcgggatg gtggtgttct
    29941 tggtgatcat cttggtcatc acgccgccca gggtctcgat gcccagggac agaggggtga
    30001 cgtccagcag cagcacgtcc ttgcggtcgc cgctcagaac ctggccctgg atggcggcgc
    30061 cgacggccac ggcctcgtcg gggttgacgt ccttgcgcgg ctccttgccg aagaactcct
    30121 tgaccttgtc ctgcaccttg ggcatgcgcg tcatgccgcc gaccaggatc acgtcgttga
    30181 tgtcggagac gctgatgccg gcgtccttga tggccgtgcg gcagggcgcg atggtgcgct
    30241 cgatcaggtc atcgaccagc gattcgagtt tggcgcgcgt gagcttgatg ttcaggtgct
    30301 tggggccggt ggcatcggcc gtgatgtagg gcaggttgat gtcggtctgc gcgctgttgg
    30361 acagctcgat cttggccttt tcggcggctt ccttcaggcg ctgcagggcc agcacgtcct
    30421 tggacagatc cacggcctgg tcctttttga actcgccgat gatgtagtcg atgatgcgct
    30481 ggtcgaagtc ctcgccgccc aggaaggtgt cgccgttggt ggccagcacc tcgaactgct
    30541 tctcgccgtc cacgtcggcg atctcgatga tggacacgtc gaaggtgcct ccgcccaggt
    30601 catacacggc gatcttgcgg tcggccttgt cctgcttgtc caggccaaag gccagggccg
    30661 cggcggtggg ctcgttgatg atgcgcttga catcgaggcc ggcgatgcgg ccggcgtcct
    30721 tggtggcctg gcgctggctg tcgttgaagt aggcgggcac cgtgatcacg gcctcggtca
    30781 ccggctcgcc aagatagtcc tcggcggtct tcttcatctt gcgcaggatt tcggcgctga
    30841 tctgctgcgc cgccagcttc tggccgcgca cttccaccca ggcgtcgccg ttgtcggcct
    30901 tggtgatggt gaagggcatc aggtcgatgt ccttctgcac ttccttctcg tcgaacttgc
    30961 gaccgatcag gcgcttgacg gcatagatgg tgttcttggg gttggtcact gcctggcgct
    31021 tggccgaggc gccgacgagg atctcgccgt cttcctggta ggcaatgatg gagggcgtgg
    31081 tgcgcgcgcc ttcgctgttc tcgatcacgc gcgtggtgtt gccttccatg atggacacgc
    31141 agctattggt ggtgcccagg tcgataccga tgatttttcc catgtttttc tccgaaggtt
    31201 ttttcaaaaa tgtggatgac ggttatgtgt ggctagcggg cctggctttc aagggggcga
    31261 gcgtgttgcg tgttgcgttt tgcgttttgc gttttgcgtt ttgcgtcaat accgcccaac
    31321 gctcaacgct caacctcact tgggcgccgc caccgtcacc agcgccgggc gcag
//
LOCUS       DAOMTQ010000011        30435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_123_length_30435_cov_26.191968, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000011 DAOMTQ010000000
VERSION     DAOMTQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 30435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30435
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123_length_30435_cov_26.191968"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PK518_02280"
     CDS             <1..821
                     /locus_tag="PK518_02280"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRM47765.1"
                     /translation="SPHVLNGSRDGQRMGDWKMVDTMIVDGLWDVYNQYHMGITAENV
                     AKAYGITREMQDALALASQQKAAAAQDAGRFADEIVGVSLAQKKGDPILFNADEYLNR
                     KTNAEALAGLRPAFDKAGSVTAGNASGINDGAAAVVVMSAKKAAALGLKPLARIAAYG
                     TSGLDPATMGMGPVPASRKALARAGWNAADVDLFELNEAFAAQACAVNKELAIDPARV
                     NVNGGAIAIGHPIGASGCRVLVTLLHEMQRRDARKGLAALCIGGGMGVSLALER"
     gene            965..1702
                     /gene="phbB"
                     /locus_tag="PK518_02285"
     CDS             965..1702
                     /gene="phbB"
                     /locus_tag="PK518_02285"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRM47766.1"
                     /translation="MSQKVAYVTGGMGGIGTAICQRLHKEGLKVIAGCGPTRDYAKWL
                     AEQKALGYTFYASAGNVGDWDSTVEAFAKAKAEHGSIDVLVNNAGITRDRMFVKMTRE
                     DWDAVIETNLNSMFNVTKQVVADMVEKGWGRIVNISSVNGEKGQAGQTNYSAAKAGMH
                     GFSMALAQELASKGVTVNTVAPGYIGTDMVKAIRPDVLEKIVGTVPVKRLGEPSEIAS
                     IVAWLASDEGGYATGAEFSVNGGLHMR"
     gene            complement(2165..4108)
                     /locus_tag="PK518_02290"
     CDS             complement(2165..4108)
                     /locus_tag="PK518_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM47767.1"
                     /translation="MITLKNVTLRRGSKVLLDGVSATINPGEQVGLVGRNGAGKSTLF
                     ALLNGTLHEDGGDFFMPPQWRMAQVAQHMPETDEPATDFVLDGDTRLSSLRDKLSKAE
                     RTGDGMAIAQAHADLQDAGAHDAPARAQALILGLGFQVAELDRPVNSFSGGWRMRLQL
                     ARALMCPSDLLLLDEPTNHLDLDALVWLEAWLKRYEGTLIVISHDREFLDAVTGVTLH
                     IDNGTLTRYGGNYSQFEELRAQQLTLQQASFERQQAKIAHLQKFIDRFKAKATKARQA
                     QSRVKALERMEKIAPVLASAEFTFEFQEPANLPNPMLALTDAAFGYRSEDGAESTILT
                     GVSKTVLAGQRIGILGANGQGKSTLVKTIARTMAPLSGKVTEGKGLAIGYFAQQELDV
                     LHPDDHPLGHMVRLARELGVDAREQDLRNFLGSFNFSGDMVLQAVGSMSGGEKARLVL
                     AMIVWQRPNLLLLDEPTNHLDLATREALAMALNEFEGTVILVSHDRALLRAVCDEFWL
                     VGRGQVGPFDGDLDDYQRYLLEESKRLREQARTVSSAVSAPAAAAPPQRDAREQRRLD
                     AQARQLLAEKTRPLKRELDKIDQRLAALGEEKAALEERLMQALPAAEIADCGRRLKAC
                     ADEIEVLEEKWLDMSTTLEELSR"
     gene            complement(4105..5016)
                     /gene="prmB"
                     /locus_tag="PK518_02295"
     CDS             complement(4105..5016)
                     /gene="prmB"
                     /locus_tag="PK518_02295"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRM47768.1"
                     /translation="MTQAVQGATVGALIQSGAEQLAQAGVVFGHGTTNAWDEAAWLVL
                     WRLGLPLDSVLEEGVADSVENRTVTPANQALVATLFEERIATRKPAAYLTREAWLQGV
                     PFYVDERAIVPRSFIAELLAGGGIDDWLSDQTRNVLDLCTGNGSLACLAAMAWPEVAV
                     TGADISPDALAVARINVDKHGLQQRVRLIESDGLAACPGPWDLILCNPPYVNAQSMAA
                     LPQEYRAEPALALAGGADGMDFVRRLLQDAPTHMSQDAVLVLEIGNERAHFEAAFPQL
                     PVFWLPTSAGEDQVLLVTRAALAHFSA"
     gene            complement(5013..6188)
                     /gene="dapE"
                     /locus_tag="PK518_02300"
     CDS             complement(5013..6188)
                     /gene="dapE"
                     /locus_tag="PK518_02300"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRM47769.1"
                     /translation="MSPTLQLTEQLIARPSITPEDAGCLDLLTERLAPLGFACERLDS
                     GPPSFRVSNLWAKRPAASVNKAQEAIKTIVFAGHTDVVPTGPVEQWGSPPFQPTQRDG
                     RLYGRGASDMKTSIAAFIVAVEEFLAATPEPAIAIAFLLTSDEEGPSVDGTKVVVERL
                     RARGERLDWCIVGEPTSVTSTGDMIKNGRRGTLSGRLAVKGVQGHIAYPQLARNPIHQ
                     AVPALAELAATEWDQGNAFFQPTSWQMSNIHAGTGATNVIPGQVVIDFNFRFSTESTA
                     DGLKARVHAVLDHHGLEYDLGWTLGGQPFLTQPGELVDAVQQAIHAETGLATELSTTG
                     GTSDGRFIAQICPEVVELGPPNDSIHKIDENIRLVDIEPLTNIYRRTLENLNARAAA"
     gene            complement(6233..7396)
                     /locus_tag="PK518_02305"
     CDS             complement(6233..7396)
                     /locus_tag="PK518_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRM47770.1"
                     /translation="MSTMERILRLMGEKKASDVYLSANAPALIKINGECVPINNQLLP
                     PDAPRNLLAEIVPPDRIEELEETGELNMGVPLAGVGRFRVSAMRQRGTYAVVIRFIAQ
                     EIPKLSTLNLPPVLSELIMEKRGLILVVGATGSGKSTTLAAMIDERNEAMTGHILTVE
                     DPVEYQFKNKKSIVNQREIGGDTQSLQTALKNALRQAPDVILIGEIRDRETMSAAIAY
                     AQSGHLCLATLHGNNSYHALNRILSFYPVEVRSTMLGDLGSALKAIISQRLVRTKTGE
                     RLPAAEVMLNTKLVADLIEQSDFSGVREAMEKSMAEGSQTFEEALAQLIMSDKIDRKE
                     GLAYADSPTNLMWRLQNDFTIAARAAKAQQEASQQPEDEEPSFTEIVLDVKPA"
     gene            complement(7592..8416)
                     /gene="dapD"
                     /locus_tag="PK518_02310"
     CDS             complement(7592..8416)
                     /gene="dapD"
                     /locus_tag="PK518_02310"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRM47771.1"
                     /translation="MTQQLQTLIDNAWDNRASLAPGAAPKEIEDAVEHVIAELNNGQL
                     RVATREGVGQWTVHQWIKKAVLLSFRLKDNAVMRAGDLAFFDKVPPKFSHLTPDEMAA
                     TGVRVVPPAVARRGSFIAKGAILMPSYVNIGAYVDEGTMVDTWATVGSCAQVGKHVHL
                     SGGVGLGGVLEPLQAGPTIIEDNCFIGARSEIVEGVIVEENSVISMGVYIGKSTPIYD
                     RATGETSYGRVPAGSVVVSGNLPKDGGRYSMYAAIIVKKVDAKTRASTSLNDLLRD"
     gene            complement(8425..9627)
                     /gene="dapC"
                     /locus_tag="PK518_02315"
     CDS             complement(8425..9627)
                     /gene="dapC"
                     /locus_tag="PK518_02315"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HRM47772.1"
                     /translation="MNPLLSRLQPYPFERLRQLFAGVTPSAQHSAISLGIGEPRHATP
                     RFIQEALAANLAGLASYPSTLGEPRLREACAAWVQRRYGIAVDAATQLLPVNGSREAL
                     FAFTQTVVDASRPGATVVCPNPFYQIYEGATLLAGANPHFAPSDPARNFAVDWDSVPD
                     AVWQRTQLLFVCSPGNPTGAVMPLAEWEKLFALSDRHGFVIASDECYSEIYFRDEPPL
                     GGLEAAAKLGRDDYRNLIAFTSLSKRSNVPGLRSGFVAGDATLIKAFLLYRTYHGSAM
                     GLAVQAASIAAWNDEAHVEDNRALYRAKFAQVTPLLDQAMQVSLPDAGFYLWAQVPEA
                     LGMSDTDFARQLLAQYNVTVLPGSYLARETDGINPGAQRVRMALVAEADECLQAAQRI
                     VQFIRSHT"
     gene            9790..11298
                     /locus_tag="PK518_02320"
     CDS             9790..11298
                     /locus_tag="PK518_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM47773.1"
                     /translation="MADLFILRPGIWLMRRLRFSAKLALLAGVAVLAQMLVAGVQGLG
                     SQVHWWVSGLAVLAVVYLAVALRISVIQDMRQLSMSMHRAAHGDLCARVQLKGADELA
                     HMGLQLDRMVLTLSAMVADIRSNAALVAHAGQTLASDNLELAGRTEQQAASLQQTVAS
                     VAQLSSAVNGNAQTAGVADQHAIELRTMAEAGTQAMARAVDSVQGIQNDARRMGEIIQ
                     VIDSIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRMLARRSAEAAREIRGLISTS
                     VQQVESSAGLIRSSGDGIASMVHGIRGVASHVSDISHSVTEQQTGLQEISAAVQQLDQ
                     ITQRNASMAGNALHQAQALESRAHTLSQAVRNFRLQQGTADEAVALVERAAALHGGYS
                     REAFLRTVSDPAQPYHDRDMYVFALDAAGTYRAFGGNPAKVGSCVQDIPGVAGEQLLH
                     AIVTQAERAPGWVEYDITHPTTGTVQTKMSFVRRAGDLYLGCGVYKSLAAAA"
     gene            complement(11306..11989)
                     /gene="rsxB"
                     /locus_tag="PK518_02325"
     CDS             complement(11306..11989)
                     /gene="rsxB"
                     /locus_tag="PK518_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HRM47774.1"
                     /translation="MSTVEAELPSLSSTEALRALAARINAALPQTQCTRCGYPDCAAY
                     ALAVASGEAGINQCPPGGAQGIARLATITGRPIEPLSAEHGQEAPRTVAVIDENWCIG
                     CTLCIKACPTDAILGLNKRMHTVIAPHCTGCELCVPACPVDCIRMENISGEATGWAAW
                     SALQADQARTRYLFRQERLQREQQTQIMPESTDRQSPADPPEAAAAVETATKQAAIAT
                     ALARARARR"
     gene            complement(12000..13310)
                     /gene="phaZ"
                     /locus_tag="PK518_02330"
     CDS             complement(12000..13310)
                     /gene="phaZ"
                     /locus_tag="PK518_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HRM47775.1"
                     /translation="MLYTIYESQRSLMEPFADLAQAAAKFYSNPLSPLSETPLAQRLS
                     AGYDLMHRLGKDYEKPEFGIRTVDVDGVSCAIHERIEIDKPFCELRRFKRFSDDPDTL
                     LKLKVQPVVLIVAPLSGHYATLLRDTVRSMLQGHKVYITDWKNARTVPMSEGDFHLDD
                     YVNYVQEFIRHLQEKYGNCHVMSVCQPTVPVLAAVSLMASRGETTPLSMTMMGGPIDA
                     RRSPTAVNNLASKRSYSWFENNVIYRVPSNFPGAGRRVYPGFMQHMGFVAMNPDRHAT
                     SHYDYFRDLIQGAGASAEAHRKFYDEYNAVLDMDAHYYLETIKTVFQDYSLVNGTWDV
                     RAPSGELQRVRPQDITTTALLTVEGELDDISGCGQTEAAQGLCSGIPPERRTHYEVKG
                     AGHYGIFSGRRWRTHVYTQVRDFILANQPSQPAAAARVQRARSA"
     gene            complement(13490..14542)
                     /locus_tag="PK518_02335"
     CDS             complement(13490..14542)
                     /locus_tag="PK518_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HRM47776.1"
                     /translation="MSSSTHLAREEAAFHAFYALERPALEQARVFFKDLLQTILAQAR
                     HIDIAKIESRVKDRDECLRKFSRKYRTTLEESGVPYEIRPYITDLIGVRVVCLYEDEL
                     EKVAQIVQSHFDVIDVTDKVSAMEGTEASFGYKGLHLDLRLNAAQAALPQHAAYVHQP
                     FELQVRTIIQDAWSVLDHKIKYKKSIPAQLKRRINVLSALFELADREFLQIRDATAAE
                     LQRAPDETTEADPPGARPALAGSELNAFTFLKIANHFFKDVEFEGQKVDQFVDDIRAW
                     SPAITRARFNALMRETLATVKRYKQYYEERNPESSFNPFTVMRHCLYLGDKQLFRRAL
                     RNTSREAFEAWLQGTS"
     gene            complement(14625..15623)
                     /gene="dusA"
                     /locus_tag="PK518_02340"
     CDS             complement(14625..15623)
                     /gene="dusA"
                     /locus_tag="PK518_02340"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HRM47777.1"
                     /translation="MQPTPPSSPHNPWRMSVAPMMDWTDRHCRHLHRLLSRHTLLYTE
                     MVTTGALLHGDVPRHLRFGEAEHPVALQLGGSEPADLTQAARLGAQWGYDEINLNCGC
                     PSERVQRGAFGACLMNEPHLVADCVKAMRDMVDIPVTVKHRIGIDQGEDYGFVRDFVG
                     TVADAGCTVFIVHARNAWLKGLSPKENREIPPLRYDVAARLKADFPYLTIAINGGFQT
                     DAAVQEQLAHVDGVMVGREAYHNPWWLARWDELFFGAAPGLLTREQVEEDMVAYMERE
                     AVEHGTHWYAIARHMLGLRHGLPGARRWRQVWSDHRLKTLPARDVMALARTRPPTT"
     gene            15827..16942
                     /locus_tag="PK518_02345"
     CDS             15827..16942
                     /locus_tag="PK518_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRM47778.1"
                     /translation="MPNHQPQGAALRTVVILGAGQIGQAIALKLLDAGGYALLVADRN
                     AASLQRLHALTGIPTAQIQDAVALEAVIAGAYAVLNALPFHQAVPVATLCVRHGVHYF
                     DLTEDVASTQAIRNLARDARSVLMPQCGLAPGFIGVVGNDLAGRLDVARSLRLRVGAL
                     PRYPQGSLRYSLTWSTEGLINEYCKPCEVIAQGRRSTVPALDGLEPLLIDGVEYEAFH
                     TSGGLGTLTQTWDGRIEQLDYKTIRYPGHRAAMQLLLTELRLSERRELLKELLEGAIA
                     GTQQDVIVILASAMGQRAGRLVQESYAARITGTRLRGTTLSAIQHTTAASICTALDLV
                     STQRLPQAGFVGQEQMALTDFLANRFGCIYDAGASQA"
     gene            complement(16949..17584)
                     /locus_tag="PK518_02350"
     CDS             complement(16949..17584)
                     /locus_tag="PK518_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM47779.1"
                     /translation="MSALTLTPRALYEQVAEQLRQRIFRRELEPGGWIDELKIAEEFG
                     ISRTPLREALKVLAAEGLVTMKVRRGAYVTEMNDKDLRDVYHLLALLESDAAGVVAAR
                     ATPEQLRELEQLHADLAAALDERERFFAINERIHMRMLEMADNRWRSQMVADLRKVMK
                     LNRHNSLLKQGRIEDSLAEHEVILAALRAHDARATVAAMRDHFAQGLKAAA"
     gene            17712..19871
                     /gene="scpA"
                     /locus_tag="PK518_02355"
     CDS             17712..19871
                     /gene="scpA"
                     /locus_tag="PK518_02355"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRM47780.1"
                     /translation="MSSDNPTFQPASLDAWVRAAAKSAPGGDVNALNWVTPDGISVKP
                     LYTAADTQDLPYANTLPGFEPYLRGPQATMYSVRPWTIRQYAGFSTAEESNAFYRKAL
                     AAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFNEIPLDKVSVS
                     MTMNGAVLPVLAGYVVAAEEQGVSQEKLSGTIQNDILKEFMVRNTYIYPPKPSMRIIG
                     DIIEYTAKNMPKFNSISISGYHMQEAGANQALELAFTLADGKEYVKTAIAKGMDVDEF
                     AGRLSFFWAIGMNFYLEIAKMRAARLLWCRIMKGFDAKNPKSLMLRTHCQTSGWSLTE
                     QDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHI
                     TNVIDPWAGSYMMEKLTQDMADAAWKIIEEVEGMGGMTAAVDSGWAKLKIEAAAAQKQ
                     ALIDSGKEVIVGVNKYKLAKEDPVDILEVDNVKVRDSQIARLNSIKQKRDAAQVQQAL
                     AALTSAAESGTGNLLDLAIKAIRLRATVGEVSDALEKSFGRHRADTQMVTGVYAQAYE
                     GAEGWEKLKGEINAFAEQQGRRPRVMIAKLGQDGHDRGAKVVSTAFADLGFDVDIGPL
                     FQTPEECARQAIENDVHAIGVSTLAAGHKTLVPAIINALKAQGADDIIVFVGGVIPAQ
                     DYDFLNAAGVKGIFGPGTPIPASAKTVLEQIKLAISA"
     gene            19910..20203
                     /locus_tag="PK518_02360"
     CDS             19910..20203
                     /locus_tag="PK518_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47781.1"
                     /translation="MAVSVRLDPLLEKDLELAAKRLGLTKSQFIVNALEKALERKNPY
                     ELLLKVEREMQADPHYARLDAAFAGEPQPAYDTEQSRAALIAKLRTKHGLSPD"
     gene            20184..20603
                     /locus_tag="PK518_02365"
     CDS             20184..20603
                     /locus_tag="PK518_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRM47782.1"
                     /translation="MASALIDASALVAVFGKNQPHAEHYRELLTLASLERWALSTTWP
                     CVIEAAYLVKAPQCFALLQWIGEGAVMVYPFDQRHLLDFVTVMQRYTESPRTEMDLAD
                     ASLYHLAIETGATRILTLDRRDFSRYRLPDGRAFEIL"
     gene            20608..21612
                     /gene="meaB"
                     /locus_tag="PK518_02370"
     CDS             20608..21612
                     /gene="meaB"
                     /locus_tag="PK518_02370"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRM47783.1"
                     /translation="MDHPLLDPLLHGAPMAQRRAMAKTITLLESTRQDHRTQGDQLLT
                     ALLPHTGRALRLGISGVPGVGKSTFIEALGLYLVRQGLKVAVLAIDPSSSVTGGSILG
                     DKTRMEQLCAQEAAYIRPSPSSGTLGGVAEKTREAMLVCEAAGYDVVIVETVGVGQSE
                     TAVHGMTDMFCVLQLPNAGDDLQAIKKGVMELADLVVINKADIDPAAATRAQAQITSS
                     LRLLGFHGNPEHDPHNAQQWTPKVLQISALQQQGVDGFWAAVQDYRQMQSANGRLAAR
                     REKQALAWMWERIDFGLKQAFRAHPQVQALLPQMQADVAAGRIAASTAARNLLAAHMG
                     "
     gene            21667..23199
                     /locus_tag="PK518_02375"
     CDS             21667..23199
                     /locus_tag="PK518_02375"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRM47784.1"
                     /translation="MQTILDQLEKKRELARLGGGQKRIDAQHAKGKLTARERIEVLLD
                     VGSFEEWDMFVEHRCADFGMDATHIPGDGVVTGYGTINGRLVFVFSQDFTVFGGALSE
                     AHAEKICKVMDQAMKVGAPVIGLNDSGGARIQEGVASLGGYAEVFQRNVMASGVIPQI
                     SMIMGPCAGGAVYSPAMTDFIFMVKDTSYMFVTGPEVVKTVTHESVTAEELGGALTHN
                     TKSGVADLAFENDVEALLMLRRFFNYIPASNREKAPHRPTQDRVDRADLSLDTLVPEN
                     PNQPYDIKEAIVKTVDDGEFFELQPDYAANIVIGMARMDGHVVGVVANQPLVLAGCLD
                     IKSSIKAARFVRFCDAFNIPVITFVDVPGFMPGTAQEYGGIIKHGAKLLYAYAECTVP
                     KITVITRKAYGGAYDVMSSKHLRGDVNLAWPNAEIAVMGAKGAVEIIFREEKKDPAKL
                     AAREAEYKARFANPFVAGSRGYIDDVIQPHETRQRICRSLAMLKDKKLDNPWRKHGNI
                     PL"
     gene            23220..23534
                     /locus_tag="PK518_02380"
     CDS             23220..23534
                     /locus_tag="PK518_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM47785.1"
                     /translation="MPKAATAQQAVKLTDIPNIGPSIAADLKGLGVHAPTDVGRMDPW
                     HTYDALRGPMGQRHDPCVLDVLLAAHEFMNGGAAKPWWAFTAQRKAQWASRFKTAAKG
                     LK"
     gene            complement(23543..23875)
                     /locus_tag="PK518_02385"
     CDS             complement(23543..23875)
                     /locus_tag="PK518_02385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM47786.1"
                     /translation="MKIERGNVYWADLDPVRGSEMAKTRPCVVVSATQFNAIRRTVVI
                     VPLTTTTAEPVWPLLIALPSFSSKTRARPEQVRVLDKSRLRKRIDTLDADALLLLDEA
                     LAAVLGLH"
     gene            complement(23872..24237)
                     /locus_tag="PK518_02390"
     CDS             complement(23872..24237)
                     /locus_tag="PK518_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47787.1"
                     /translation="MMHTLNAPPRQVLLRLPEDLAAKLARAVAPRQRNRFLVDLLAKG
                     LAEQEEARNQVLIDAANRMNELEAKHPELLQEAREWEAAELTDSVDVWDADFDREMFE
                     RDLAEAQKTRNQSALPAKR"
     gene            24290..26350
                     /gene="accC"
                     /locus_tag="PK518_02395"
     CDS             24290..26350
                     /gene="accC"
                     /locus_tag="PK518_02395"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRM47788.1"
                     /translation="MFTKILIANRGEIACRIITTAKRMGIATVAVYSDADKNARFVQL
                     ADEAVRLGPPPSRESYLLADKIIEACKQTGAQAVHPGYGFLSENAEFAKKVEEQGIAF
                     IGPKHAAIAAMGDKIASKKLAGEARVSTIPGYNDAIETPEKAVEIAKGIGYPVMIKAS
                     AGGGGKGLRVAFNDKEAYEGFASCKHEALSAFGDDRVFIEKFVEQPRHIEIQVLGDAH
                     GNVVYLNERECSIQRRHQKVIEEAPSPFISAATRKAMGEQAVQLAKAVGYQSAGTVEF
                     VVGKDQDFYFLEMNTRLQVEHPVTELITGLDLVEQMIRVAAGEKLAFTQADVKCEGWA
                     MECRINAEDPFRGFLPSTGRLVRFVPPAQDLHQGLPATGLGVRVDTGVLDGGEIPMYY
                     DSMIAKLIVHGKDRADAIARMREALNGFVIKGVQSNIPFQAALLAHPDFVSGNFNTGF
                     IADHYAHGFRAEDVPHEDPSFLLALAAHLHMFYRNRASGLQGQFWEGFKRLPRREFSV
                     CVLGEGGHNSYTRATVAETEPGHATPVTLHLPGGDKTYHLSVDRHLGEVRITGTVDGK
                     AFTAQVERGKTSNPLAIRVIHNGTRLDAIVMHPRTADLHRLMPYKAPPDLSKFVLSPM
                     PGLLVQVAVQPGQEVKAGERVAVIEAMKMENVLLASADGVVKEIKAQKGDSLAVDQAI
                     VEFE"
     gene            26553..27014
                     /locus_tag="PK518_02400"
     CDS             26553..27014
                     /locus_tag="PK518_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM47789.1"
                     /translation="MTRPFKVLGIQQVAIGGTDKGRMKKLWVDMLGLEQTGSFQSERE
                     NVDEDILAMGRGAHKVEVDIMQPLDIDKKPAVHTTPLNHIGLWIDDLPKAVEWLTAQG
                     VRFAPGGIRKGAAGYDITFLHPKSNDEFPIAGEGVLIELVQAPPEVIAALG"
     gene            27063..28856
                     /locus_tag="PK518_02405"
     CDS             27063..28856
                     /locus_tag="PK518_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM47790.1"
                     /translation="MSTAAPRPSPAAPRGQPRALGALLPFLRPYRWPIAAALAFLLLA
                     ALATLAFPLALRQLIDQGGSSQAAALRDNFGLLFAVAVAVGLFSAARYYAMSWLGERV
                     TADLRSRVYAHVLTQSPAFFETTQTGEVLSRLTADTTLVQTVIGSSFSLGLRNAVLGL
                     GALAMLVWTNPVVMSAALAAVLMVVLPTMWIGRSVRRLSRASQDRVADASAIAAEVLG
                     AIPIVQGFTAERREAARFAASAEAAFQTAVRRSGVRALLVAFIIIANAALLLWGLYRG
                     SQAVLAGQMSAGSLGQTIVYALFLAGAVAVLGEVYGDLLRAAGAMERLMELLATRPAI
                     RSPEAGTLQGLAPGQPLAVAFEAVDFHYPSRPDRAALAGFTLHLAPGETVALVGASGA
                     GKSTVFQLLQRFYDVQAGRILLGGQDIRGLDLHALRGRIATVPQDAVIFSSSALDNIR
                     YARPEATREEVINAARAAFAHDFIEALPQGYDSFLGEHGVRLSGGQRQRIAIARALLK
                     DAPVLLLDEATSALDAHSERVVQQALDAALQSRAGSRITLVIAHRLATVQHADRIVVL
                     DHGCIVEQGSHAALMAADGAYARLAALQFTA"
     gene            29145..30206
                     /gene="bioB"
                     /locus_tag="PK518_02410"
     CDS             29145..30206
                     /gene="bioB"
                     /locus_tag="PK518_02410"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRM47791.1"
                     /translation="MTTVFLADAPTPANHARPAHASPAAAQRWSVQAVQELLDRPFMD
                     LLWQAQEVHRQHWPAGDIELATLLSVKTGGCAENCGYCPQSSHFDTGVQAQKLMAVDE
                     VRSAAQAAKNAGATRFCMGAAWRAPKERDIEKMSELIRTVKGLGLQTCATLGMLQPHQ
                     AQALKAAGLDYYNHNLDTAPEYYQDVVSTRSYQDRLDTLRHVRAAGVKVCCGGIVGMG
                     EAPVHRAGLIAQLANLDPYPESVPINSLVPVPGTPLAGSAPVDSLDFVRVIAVARITM
                     PRARVRLSAGREQLGEAVQALCFLAGANSIFYGEKLLVTGNPDVAADERLLAKLGLRA
                     HATHGQAQPAGTDRGCACS"
     gene            complement(30284..>30435)
                     /locus_tag="PK518_02415"
     CDS             complement(30284..>30435)
                     /locus_tag="PK518_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRM47792.1"
                     /translation="LGIPGSPIFASQWWLAFTAFVGANLLQSGLTKWCLLETMLRKLG
                     IQPGC"
BASE COUNT         5321 a         9626 c        10144 g         5344 t
ORIGIN      
        1 tgtcgcccca tgtcctgaac ggctcgcgcg acggccagcg catgggcgac tggaaaatgg
       61 tggacaccat gatcgtggac ggcctgtggg acgtctacaa ccagtaccac atgggtatca
      121 ccgccgagaa cgtggccaag gcctacggca tcacgcgcga gatgcaggat gcgctggccc
      181 tggccagcca gcaaaaggcc gccgcggccc aggacgccgg ccgctttgcc gacgaaatcg
      241 tcggcgtctc gctggcgcag aaaaagggcg acccaattct gttcaatgcc gacgaatacc
      301 tgaaccgcaa gaccaatgcc gaagccctgg ccggtctgcg cccggcgttt gacaaggccg
      361 ggtcggtgac ggccggcaat gcctcgggca tcaacgacgg cgccgccgcc gtggtggtca
      421 tgagcgccaa gaaggcggcg gccctgggcc tcaagcccct ggcgcgcatc gccgcctacg
      481 gcacctccgg cctggatccg gccaccatgg gcatgggccc ggtgccggca tcgcgcaagg
      541 cactggcccg cgccggctgg aacgccgccg atgtcgacct gttcgagctc aacgaagcct
      601 tcgccgccca ggcctgcgcc gtcaacaagg agctggccat cgacccggcg cgtgtcaacg
      661 tcaacggcgg ggccattgcc atcggtcacc ccatcggcgc ctcgggctgc cgcgtgctgg
      721 tgacgctgct gcacgagatg cagcgccgcg acgccaggaa gggcctggct gcgctgtgca
      781 ttggcggcgg catgggcgtg tcgctggcgc tggagcgcta agccgtcaga gattcgggcc
      841 ttcgcgtcat tgttgattca gtcgcctggg cgacaatgac caaggcccgt gtttaccttg
      901 atcgcacaga tcaggatctt gggtagagtg tttacggttt tttccaccac acaaggagca
      961 cacaatgagt cagaaagtcg catacgtcac cggaggcatg ggtggaatcg gcacggccat
     1021 ttgccaacgc ctgcacaagg aaggcttgaa ggtcatcgct ggctgcggcc ccacgcgcga
     1081 ctacgccaag tggctggccg agcagaaggc cctgggctac accttctacg cctctgcagg
     1141 caatgtgggt gactgggatt ccacggtgga ggccttcgcc aaggccaagg ccgagcatgg
     1201 cagcatcgat gtgctggtga acaacgccgg catcacgcgc gaccgcatgt tcgtgaagat
     1261 gacgcgcgag gactgggacg ccgtcatcga aaccaacctc aacagcatgt tcaacgtgac
     1321 caagcaggtt gttgccgaca tggtggaaaa gggctggggg cgcatcgtca acatcagctc
     1381 ggtcaatggg gaaaagggcc aggccggcca gaccaactac tcggccgcca aggccggcat
     1441 gcacggcttc tccatggcgc tggcgcagga actggcctcc aagggcgtaa cggtgaatac
     1501 cgtggcgccg ggctatatcg gtaccgacat ggtcaaggcg attcgccccg atgtgctgga
     1561 gaagattgtc ggcaccgtcc ccgtcaagcg cctgggcgag cccagcgaaa ttgcctccat
     1621 cgtcgcctgg ctggcctctg acgagggtgg ctacgccact ggcgcagagt tctccgtcaa
     1681 cggtggccta cacatgcgct gatacagtga agagcattcc cggcaactgc tgccgggaat
     1741 gggccataaa aaaaaggaac gtcaccagac gttccttttt tttcgggcca ccggtgcact
     1801 accgcacttc gacttgaacc cggtgggccg cgccgcccac gcaggactgg cgcacagggg
     1861 gagcctgtca actcccgaaa tcagggcctc gcaaggccgc aggatgccct tacaggcggg
     1921 ttggcgcgcg cttgcgatcg cgttcgtctt cgggccaggc agagagcatt gcagtcatgg
     1981 tcatgggaaa aactcctttg atgtaaccac aacgcgaacc ccgggcgcag cgttgacagc
     2041 gagtggaaag aaagtgcaac ccctggatag cacctgtttt tgctctcgat aaaagagcta
     2101 taaatccatg ctgcaaaagc gctaacgggc aatttcttcc gtgtgttttc ctggcaggcc
     2161 gccgctatcg gctcaattct tcaagagtcg tggacatatc cagccacttt tcttccagta
     2221 cttcgatctc gtcggcgcag gctttcaggc gccgcccgca gtcggcaatc tctgccgctg
     2281 gcagggcttg catcaggcgc tcttccagcg ctgccttttc ctcgcccagg gctgccaggc
     2341 gctggtcgat cttgtcgagt tcgcgcttca gcggccgagt cttctcagcc aacaactggc
     2401 gcgcctgggc gtccaggcgg cgctgttcgc gtgcgtcacg ctggggtggc gcagcggccg
     2461 ccggcgccga taccgcgctg ctgaccgtgc gggcctgctc gcgcagacgc ttggattctt
     2521 ccagcaggta gcgctggtaa tcatcgaggt cgccatcaaa ggggccgacc tggccgcggc
     2581 cgacaagcca gaactcgtcg cacacggcgc gcagcagggc ccggtcgtgg ctcaccagga
     2641 tcaccgtgcc ctcgaactcg ttgagcgcca tggccagggc ctcgcgcgtg gccaggtcca
     2701 ggtggttggt gggctcgtcg agcagcagca gattcggccg ctgccagacg atcatggcca
     2761 gcaccaggcg cgccttttcg ccgccgctca tgctgcccac ggcctgcagc accatgtcgc
     2821 cgctgaaatt gaatgacccg aggaagttgc gcaaatcctg ttcgcgcgcg tccacgccca
     2881 gctcgcgcgc caggcgcacc atgtgcccca gcgggtgatc atccgggtgc agcacatcga
     2941 gttcctgctg ggcgaagtag ccaatcgcca ggcctttgcc ctcggtgacc ttgcccgaga
     3001 gcggcgccat ggtgcgggcg atggttttga ccagcgtgga cttgccctgg ccgttggcgc
     3061 ccagaatgcc tatgcgctgg ccggccagca ccgttttgct gacgccggtg aggatggtgc
     3121 tctcggcgcc gtcttcactg cggtagccaa aggccgcatc ggtcagcgcc agcatggggt
     3181 tgggcaggtt ggccggctcc tggaattcaa aggtgaattc ggcgctggct agcaccgggg
     3241 cgatcttttc catgcgctcc agcgccttga cgcggctttg cgcctggcgt gccttggtgg
     3301 ccttggcctt gaagcggtcg atgaacttct gcaggtgggc gatctttgcc tgctggcgct
     3361 cgaacgacgc ctgctgcagg gtcagctgct gcgcccgcag ctcctcgaac tggctgtagt
     3421 tgccgccgta gcgcgtgagc gtaccgttgt cgatgtgcag cgtgacgccg gtcacggcat
     3481 ccagaaactc gcggtcgtgg ctgatgacga tgagcgtgcc ctcgtagcgc ttgagccagg
     3541 cctccagcca caccagcgcg tccagatcca ggtggttagt gggctcgtcc agcagcagca
     3601 ggtcggacgg gcacatcagc gcgcgcgcca gctgcaggcg catgcgccag ccgccggaga
     3661 agctgttcac ggggcgatcg agttcggcca cctgaaaccc caggcccagg atcagcgcct
     3721 gcgcgcgtgc gggcgcgtca tgcgcgccgg catcctgcag gtcggcatgc gcctgggcga
     3781 tggccatgcc atcgccggta cgctctgctt ttgatagctt atcgcgtaga ctggacaagc
     3841 gtgtatcgcc atccagcaca aaatccgtgg ccggctcgtc ggtctcgggc atatgctgcg
     3901 cgacttgcgc catgcgccac tgcggcggca tgaagaaatc gccgccgtct tcgtgcagcg
     3961 tgccgttcaa gagcgcaaac agcgtggact tgcccgcgcc gttgcggcct accaggccga
     4021 cctgttcacc cggattgatg gtggcgctga cgccatcgag gagcaccttg ctgccgcggc
     4081 gcaaggtgac gtttttcaga gtgatcatgc agaaaaatgc gccagggcgg cacgcgtgac
     4141 gagcagcacc tggtcttcgc cggcactggt gggcaaccag aacacgggca attgggggaa
     4201 ggcggcctcg aagtgggcgc gctcgttgcc gatctccagc accagcacgg catcctggct
     4261 catgtgcgtg ggggcgtcct gcagcaggcg gcggacgaag tccatgccgt ctgcgccgcc
     4321 ggccagggcc agcgcgggct cggcgcggta ctcctgcggc agggcggcca tgctctgggc
     4381 gttgacatag ggcgggttgc acaggatcag gtcccagggg ccggggcagg cggccaggcc
     4441 gtcggactcg atcaggcgca cgcgctgctg caggccatgt ttgtcgacgt tgatgcgcgc
     4501 cacggccagg gcgtccggcg agatgtcggc gccggtcact gctacctcgg gccaggccat
     4561 ggccgccaga caggccaggc tgccgttgcc ggtacacagg tcgagcacgt tcctggtctg
     4621 gtcgctgagc cagtcgtcga tgccgccgcc tgccagcagc tcggcgatga agctgcgcgg
     4681 cacgatggcg cgctcgtcca cgtagaaggg cacgccctgc agccaggcct cgcgcgtcag
     4741 gtaggcggcg ggcttgcgcg tggcgatgcg ctcttcaaaa agagtagcta ccagcgcttg
     4801 attggcgggc gttacggttc gattttcaac tgaatcagcc acaccttctt cgagtacgct
     4861 gtccagcggc aggcccaggc gccacagcac cagccaggcg gcctcgtccc aggcgttggt
     4921 cgtgccatgg ccaaagacca cgccggcctg ggccagctgt tccgcaccgc tttggatcag
     4981 cgcgcccacg gtggcgcctt gtacggcctg tgtcatgccg cggcccgcgc gttcaggttc
     5041 tccagcgtgc ggcggtagat gttcgtcagg ggctctatgt ccaccaggcg gatgttctcg
     5101 tcgatcttgt ggatgctgtc gttgggcggg ccgagttcga ccacctcggg gcagatctgg
     5161 gcgatgaagc ggccgtcgct ggtgccgccg gtggtggaca gctcggtggc caggccggtc
     5221 tcggcatgga tcgcctgctg cacggcatcg accagctcgc ccggctgcgt cagaaagggc
     5281 tggccgccca gcgtccagcc caggtcgtac tccaggccgt ggtggtcgag cacggcatgc
     5341 acgcgcgcct tcaggccgtc ggcggtggat tcggtgctga agcggaagtt gaagtcgatc
     5401 accacctggc cggggatgac gttggtcgca cccgtgccgg cgtggatgtt gctcatctgc
     5461 cagctggtcg gctggaagaa ggcgttgccc tggtcccact cggtggcggc cagctcggcc
     5521 agcgcgggca ccgcctggtg gatggggttg cgcgccagct gcgggtaggc gatatggcct
     5581 tgcacgccct tcacggcgag ccggcccgag agcgtgccgc gacggccgtt cttgatcatg
     5641 tcgccggtgc tcgtgaccga tgtcggttcg cccacgatgc accagtccag gcgctcgccg
     5701 cgtgcgcgca gcctctcgac caccaccttc gtgccgtcca ccgacgggcc ttcctcgtcg
     5761 ctggtgagca aaaaggcgat ggcgatggcc ggctcgggcg tggcggccag gaactcctcg
     5821 accgcgacga tgaaggcggc gatcgacgtc ttcatgtcgc tggcaccgcg cccgtacagc
     5881 cggccgtcgc gctgcgtggg ctggaacggc gggctgcccc attgctccac ggggccggtg
     5941 ggcaccacgt cggtatggcc ggcaaacact attgttttaa tagcttcttg cgctttattg
     6001 acggacgctg caggcctttt tgcccataga ttgctgacgc ggaagctggg cggcccgctg
     6061 tccaggcgct cgcaggcaaa gcccagcggg gccaggcgtt cggtcagcag atccaggcag
     6121 cccgcgtcct cgggggtgat ggaggggcgg gcgatgagtt gttcggtcag ttgcagggtg
     6181 ggggacatcg ggtgggcggt agagaggccg gcgcgcgagc cggctggggc cgtcaggcag
     6241 gcttgacgtc cagcacgatc tcggtgaacg aaggctcttc gtcctcgggc tgctggctcg
     6301 cctcctgctg cgccttggcg gcacgggcgg caatggtgaa gtcgttttgc agacgccaca
     6361 tgaggttggt gggcgagtcg gcgtaggcca gaccttcctt gcggtcgatc ttgtcgctca
     6421 tgatcagctg cgccagggct tcctcgaagg tctgcgagcc ttcggccatg gatttttcca
     6481 tggcctcgcg cacgccggag aagtcgctct gctcgatcag gtcggccacc agcttggtgt
     6541 tgagcatgac ctcggccgcc ggcaggcgct cgcccgtctt ggtgcgcacc aggcgctgcg
     6601 agatgatggc cttgagggcc gagcccaggt cgcccagcat ggtggagcgc acctcgactg
     6661 ggtagaacga caaaatgcgg ttgagcgcgt ggtagctgtt gttgccgtgc agcgtggcca
     6721 ggcacaaatg gcccgactgg gcataggcga tggccgccga catggtctcg cggtcgcgga
     6781 tctcgccaat caggatcacg tccggcgcct ggcgcagggc gttcttcaac gccgtctgca
     6841 gcgactgcgt gtcgccgccg atctcgcgct ggttgacgat ggacttcttg ttcttgaact
     6901 ggtattcgac cgggtcttcg acggtgagga tatggcccgt catggcctcg ttgcgctcgt
     6961 cgatcatggc cgccagtgtc gtgctcttgc ctgaaccggt ggcgccgacg accaggatca
     7021 ggccgcgctt ttccatgatc agctcgctga gcacgggcgg cagattcagg gtggacagct
     7081 tgggtatctc ttgggcgatg aaacggatca ccaccgcgta ggtgccgcgc tggcgcatgg
     7141 cgctgacacg aaagcgcccc acgccagcga gcgggacacc catgttgagc tctcccgtct
     7201 cctccagctc ctcgatgcgg tcgggcggca cgatctcggc cagcaggttg cgcggcgcat
     7261 cgggcggcaa cagctggtta ttgatgggca cgcattcacc gttgatcttg atgagggccg
     7321 gcgcattcgc cgacaggtag acgtcggagg ccttcttctc gcccatcagg cgcaatatgc
     7381 gttccatcgt gctcatgctt gctccctctc tcgcggtcgt gtgtattggt tatccactgc
     7441 ggttcgcgcg tcgcccgcga cgcatgcaac tggcgcggcc ccacgcagcg gacgccgcgc
     7501 aagggccgcc ccgccgcgct ggcgtcgtcc ccctgcccgc gaagcgagag cgggggtgaa
     7561 ggcgcgaagc gcctcagggg ggtgtcgcat atcaatcgcg caacaggtcg ttcaggctgg
     7621 tggaggcgcg cgtcttggcg tccaccttct tgacgatgat ggccgcgtac atgctgtagc
     7681 gcccgccatc cttgggcagg ttgccgctga ccaccaccga gcccgcgggc acgcggccgt
     7741 agctggtttc gcccgtggcg cggtcgtaga tcggggtgct cttgccgatg tacacgccca
     7801 tggagatgac ggagttttcc tcgacgatca cgccctcgac gatctcggag cgcgcgccga
     7861 tgaagcagtt gtcttcgatg atggtggggc cggcctgcag tggctccagc acaccgccca
     7921 agcccacgcc gcccgacagg tgcacatgct tgcccacctg ggcgcaggag cccacggtgg
     7981 cccaggtatc gaccatggtg ccctcatcca cataggcgcc gatgttcacg tagctgggca
     8041 tgaggatggc acccttggcg atgaagctgc cgcggcgcgc cacggccggc ggcaccacgc
     8101 gcacgccggt ggcggccatc tcgtcgggcg tgaggtgcga gaacttcggc ggcaccttgt
     8161 cgaagaaggc caggtcgccg gcgcgcatga cggcgttgtc cttcaggcgg aaggacagca
     8221 gcacggcctt tttgatccac tggtgcaccg tccactggcc cacgccttca cgcgtggcca
     8281 cgcgcaactg gccgttgttg agctcggcga tcacatgctc gacggcatct tcgatctctt
     8341 tgggggcggc accgggcgcc aggctggcgc ggttgtccca ggcgttgtct atcagggtct
     8401 gcagttgttg tgtcatgaaa ggaatcaggt atgggagcgg atgaattgca cgatgcgctg
     8461 ggcggcctgc aggcactcgt cggcctcggc caccagggcc atgcgcacgc gctgggcgcc
     8521 aggattgatg ccatccgtct cgcgcgccag gtagctgccc ggcagcaccg tcacattgta
     8581 ttgagccagc agttggcggg cgaagtcggt atcgctcatg cccagcgcct cgggcacctg
     8641 ggcccagagg tagaagccgg cgtcgggcag gctgacctgc atggcctggt ccagcagcgg
     8701 cgtgacctgg gcaaacttgg cacggtacag ggcgcggttg tcctccacat gggcctcgtc
     8761 gttccatgcg gctatgctgg ccgcctgcac cgccaggccc atggcgctgc cgtggtaggt
     8821 gcggtagagc aaaaaggcct tgatgagcgt ggcgtcgccg gcgacaaagc cgctgcgcag
     8881 gccgggcacg ttgctgcgct tggacaggct ggtgaaggcg atcaggttgc ggtaatcgtc
     8941 gcgccccagt ttggccgcag cctccaggcc acccaaaggg ggctcgtcgc gaaagtaaat
     9001 ttcgctgtag cactcatcag aggcaatcac gaagccatgg cggtcgctga gcgcaaacag
     9061 cttctcccac tcggccaagg gcatcacggc gcccgtgggg ttgccgggag agcacacgaa
     9121 cagcagctgc gttcgctgcc acacggcatc gggcacgctg tcccagtcaa cggcaaagtt
     9181 gcgcgccggg tcactggggg cgaaatgcgg gttggcgccg gccagcaggg tggcaccttc
     9241 gtagatttga tagaacggat tggggcagac cacggtggca ccgggccgcg aggcatcgac
     9301 caccgtttgt gtgaacgcga ataaggcctc acgcgagccg ttgacgggca gcagctgtgt
     9361 agccgcatcc acggcaatgc catagcggcg ttgcacccag gccgcgcagg cctcgcgcaa
     9421 gcgcggctcg cccaaggttg atgggtagct ggccaggccc gccaggttgg cggcaagcgc
     9481 ttcctggatg aaacgcggtg tggcgtggcg cggctcgcca atgcccaggc tgatggcact
     9541 gtgctgcgca gacggggtaa cccccgcgaa aagctgtcgc agccgctcga atgggtaggg
     9601 ctgcagtcgg gagagcaggg ggttcatggt gccgattatc aaggatggcc agcaatgtct
     9661 tggagcgtac tcgggtgcct tgaccgagtc ggccttagcc tcgatttctg cattgattga
     9721 atgtgtttac attgttacca tcggaatcat aatcatatgg cgctctgaat tgcgagggag
     9781 ttcgagacga tggcagattt gttcattctg cgaccaggca tctggctgat gcgtcgtctg
     9841 cgctttagcg ccaaattggc cctgctggct ggtgtcgccg tgctggcgca aatgcttgtt
     9901 gcaggcgtgc aggggctggg ttcgcaggtg cactggtggg tgtccggctt ggctgtgttg
     9961 gcggtcgtgt atctggcagt cgccttgcgg atcagcgtga tccaggacat gcggcagttg
    10021 agcatgtcaa tgcatcgggc ggcacatggc gatctgtgcg cgcgcgtgca gctgaagggt
    10081 gcggatgaac tggcgcacat gggcttgcaa ctcgatcgca tggtgctcac gttgtccgcc
    10141 atggtggccg acatacgcag caacgccgcc ttggtcgcgc atgcggggca gaccctggcg
    10201 tcggacaacc tggagctcgc aggccgcacc gagcagcagg cggcaagcct gcagcagacg
    10261 gttgccagcg tggcccagct gtcctctgca gtgaatggca acgcacagac cgcgggcgtg
    10321 gcggatcaac atgccattga gctgcgcacc atggccgagg ccggcacgca ggccatggcg
    10381 cgtgcggtgg attcggttca gggtattcag aatgatgccc gtcgcatggg cgagatcatc
    10441 caggtgatcg acagcattgc ctttcagacc aatatcctgg ccctcaatgc ggctgttgaa
    10501 gccgcccggg caggggaaca gggccgagga tttgccgtgg tggcggccga ggtacgcatg
    10561 ctcgcgcgtc ggtccgccga ggcggcacgc gagatccgcg gactgatcag cacctcggta
    10621 caacaggtcg agagcagtgc cggcctgatt cgcagctccg gcgacggtat tgcttccatg
    10681 gtgcacggca ttcgtggcgt ggcaagccat gtgagtgata tttcgcattc ggtgaccgaa
    10741 cagcagaccg gtctgcaaga aatcagcgcg gcggtgcagc agcttgacca gatcacccag
    10801 cgcaacgcgt ccatggcggg caatgccctg catcaagccc aggcactgga aagtcgtgcc
    10861 cacaccctgt cacaggccgt gcggaatttc cgcctgcagc agggtacggc cgatgaagca
    10921 gtggccttgg tcgaacgagc ggcggcgctg cacggtggct attcgcgcga ggcctttctg
    10981 cgcaccgtga gcgatcctgc gcagccctac catgaccgcg acatgtatgt gtttgccctg
    11041 gatgccgccg gaacctaccg agcatttgga ggtaaccccg ctaaggttgg cagctgcgta
    11101 caggacatcc ctggggttgc gggcgaacag ctgctgcacg ccatcgtgac tcaggccgag
    11161 cgggccccgg gttgggtgga atacgacatc acgcacccga caacgggcac ggttcaaacc
    11221 aagatgtcct ttgtgcggcg cgcgggggat ttgtatcttg gttgcggggt ctacaaatcg
    11281 ctcgcggctg cggcgtagcc ctgcttcagc gcctggcccg cgcacgggcc aatgcggtag
    11341 cgatggcggc ttgcttggtg gcggtttcca cagcagctgc cgcctcaggc ggatcggctg
    11401 gtgactgtct gtccgtggac tccggcatga tctgcgtctg ctgctcccgc tgcaggcgtt
    11461 cttgccggaa cagatagcgg gtacgggcct gatcggcttg caaggctgac caggcggccc
    11521 agcctgtggc ctcgccgctg atgttttcca tgcgaatgca gtccacgggg caggcgggaa
    11581 cgcacagttc gcagcccgtg caatgcgggg caatgacagt gtgcatgcgc ttgttcaggc
    11641 ccaggatcgc atccgtgggg caggccttga tgcacagcgt gcagcctatg caccagtttt
    11701 cgtcgatgac ggccacggtg cgaggtgctt cctgaccatg ctcggcactc aagggctcaa
    11761 tcgggcggcc tgtgatggtc gccaggcgcg caatgccctg ggcgcctccg ggcgggcatt
    11821 ggttgatgcc ggcctcgcca ctggcgacgg ccagggcgta tgcagcgcag tcgggatagc
    11881 cgcagcgcgt gcactgggtc tggggcaggg cggcgttgat gcgtgccgcc agggcccgca
    11941 gcgcctctgt cgatgacagc gacggcagct cggcctcgac ggtggacatg acgcgctact
    12001 caggcgctgc gcgcacgctg cacgcgcgcg gccgcagctg gctggctggg ttggttggcg
    12061 agaatgaagt cgcgcacctg ggtgtacaca tgcgtgcgcc agcgccggcc gctgaagatg
    12121 ccgtagtggc ccgcaccctt gacttcgtag tgcgtgcggc gttcgggggg aatgccgctg
    12181 cacaggccct gtgcggcctc ggtctggccg cagccggaga tgtcgtcgag ttcgccctcg
    12241 acggtcagca gcgccgtggt ggtgatgtcc tgcgggcgca cgcgctggag ctcaccgctg
    12301 ggcgcgcgca catcccaggt gccgttgacc aggctgtagt cctggaaaac ggtcttgatg
    12361 gtctccaggt aatagtgggc gtccatgtcg agcacggcgt tgtactcgtc atagaacttg
    12421 cggtgggcct cggcgctggc gcccgcaccc tggatcaaat ccctgaagta gtcgtagtgg
    12481 ctggtcgcgt ggcgatcggg attcatggcc acgaagccca tgtgctgcat gaaacccgga
    12541 tagacgcgac gacccgctcc cgggaaattg ctcggcacgc ggtagatgac gttgttttca
    12601 aaccatgaat agctacgttt ggaggcgagg ttgttcaccg ccgtgggcga gcgacgcgcg
    12661 tcgatcgggc cgcccatcat ggtcatggac agcggcgtgg tttcgccccg gctggccatc
    12721 agcgacaccg cggcgagcac cggcaccgtt ggctggcaca cgctcatcac atggcagttg
    12781 ccgtattttt cctgcaggtg acgaatgaac tcctgtacgt agttcacata gtcgtcgagg
    12841 tggaagtcgc cctcggacat gggcacggtg cgtgcgtttt tccagtcggt gatatagacc
    12901 ttgtggccct gcagcatgga gcgcaccgtg tcgcgcagca gcgtggcgta atgcccggaa
    12961 agcggcgcga cgatgagcac cacgggttgt accttgagct tgagcaaggt gtccggatcg
    13021 tcggagaagc gcttgaaacg gcgcagctcg cagaacggct tgtcgatttc gatgcgctcg
    13081 tgaatggcgc agctgacacc gtccacgtcc acggtacgga tgccaaactc gggcttttca
    13141 tagtccttgc ccaggcgatg catgagatcg tagccggcgg acaggcgctg ggccagtggc
    13201 gtctcactga ggggcgacag cggattgctg tagaacttgg cggccgcttg tgccagatcg
    13261 gcaaagggct ccatcaggga gcgctgggat tcgtagatgg tgtacagcat atgggcgata
    13321 ttttatgttg cagtgcacaa tatgcaagca aatcgctgtg tatgtgtgta ggactaccac
    13381 gaattttacg tctcgtaggc gacaaaacaa cccaactgtt gactgggggg ttgatggcaa
    13441 atcgcgcttt gtggtagggg attgatcctt ttctggtgcc ggcttgggat cagctggttc
    13501 cttgcagcca ggcttcgaag gcctcacgcg aagtgttgcg taaagcacga cggaacagct
    13561 gcttgtcgcc cagatacagg cagtggcgca tcaccgtgaa ggggttgaag ctgctctcgg
    13621 ggttgcgctc ttcgtaatac tgcttgtagc gcttgacggt ggccagggtt tcgcgcatca
    13681 gtgcattgaa gcgggcacgg gtgatggcgg gtgaccaggc gcggatgtcg tccacgaact
    13741 gatcgacctt ctggccctcg aattccacat ccttgaagaa gtggttggcg atctttaaaa
    13801 aggtgaaagc attgagctcg ctgcccgcta gcgcgggccg ggcgccgggc gggtcggcct
    13861 cggtggtctc gtcgggcgcg cgttgcagct cggccgccgt cgcgtcgcgg atctgaagaa
    13921 actcgcggtc ggccagctcg aacagcgccg acagcacgtt gatacggcgc ttgagctgag
    13981 ccggaatcga ttttttgtac ttgatcttgt gatccagcac gctccacgcg tcctggatga
    14041 tagtgcgcac ctgtaactcg aagggctgat ggacataagc agcatgctgc ggcaaggcgg
    14101 cctgggcggc gttcaggcgc agatccagat gcaggccctt gtagccgaaa gaggcctcgg
    14161 tgccctccat ggcgctgacc ttgtcggtta cgtcgatcac gtcgaagtgc gattgcacga
    14221 tctgcgccac cttctccagc tcgtcctcat acaggcagac cacgcgcacg cctatcaggt
    14281 cggtgatgta ggggcggatt tcatagggaa cgccgctttc ttccagcgtc gtgcggtact
    14341 tgcgcgagaa cttgcgcaga cattcgtcgc ggtctttcac gcggctttcg atcttggcta
    14401 tgtcgatgtg gcgcgcctgc gcaagaatgg tctgcagcaa atccttgaaa aagacgcggg
    14461 cctgctccag ggccggacgc tccagcgcat agaaggcgtg gaaggcggct tcctcgcgtg
    14521 caagatgggt ggaagaagac atgcggtggt aggtgccgtc gggggtgatg gccaccatcg
    14581 tagggcagcc ggtcgctgcc tgccaatggt gggcggcagt gtgttcacgt cgttggtggc
    14641 ctggtgcgcg ccagggccat gacatcgcgc gccggcagtg tcttcaggcg atggtcgctc
    14701 cacacctggc gccagcggcg cgcaccgggc agaccatggc gcaggcccag catgtggcgc
    14761 gcaatcgcgt accaatgcgt gccgtgttct acggcctcgc gctccatgta ggccaccatg
    14821 tcttcctcga cctgctcgcg tgtcagcagg cccggcgccg caccaaagaa cagctcgtcc
    14881 cagcgggcca accaccaggg gttgtgatag gcctcgcggc ccaccatcac gccatcgaca
    14941 tgcgccagct gttcctggac ggcggcatcg gtctgaaagc cgccgttgat ggcaatggtc
    15001 aggtacggga agtcggcctt caggcgtgcg gcgacgtcgt agcgcagggg cggaatctca
    15061 cggttttcct tcggtgacag gcccttgagc caggcattgc gcgcatgcac gatgaacacg
    15121 gtgcaacccg cgtcggccac ggtgccgaca aagtcgcgca caaaaccata gtcttccccc
    15181 tggtctatgc caatgcggtg cttcacggtg acgggaatgt ccaccatgtc ccgcatggct
    15241 ttcacgcagt cggccaccag gtgcggctcg ttcatcaggc aggcgccaaa ggcgccgcgc
    15301 tgcacgcgct cgctggggca gccgcagttc aggttgatct cgtcatagcc ccattgcgcg
    15361 cccaggcggg cggcctgcgt caggtcggcg ggctcgctgc cgcccagctg cagcgccacg
    15421 gggtgctcgg cctcgccaaa gcgcaggtgg cgcggcacgt cgccatgcag cagcgcgccg
    15481 gtggtcacca tctcggtata gagcagggtg tggcgcgaca gcaggcggtg caggtggcgg
    15541 caatgcctgt cggtccagtc catcatcggc gcaacggaca tgcgccaggg gttgtgtggg
    15601 gaggagggag gggtgggctg cacgaaagat cttggcgatg ggtggcgcag tattgtccca
    15661 ggtaccgcat cacggatgca ggcgggcggt tttatgaggt attttcggct ttcacgcccg
    15721 tagtacaagc gccataagct attaaaataa tagcaacctc tagtcacagg gcgcaagccc
    15781 gcctaccatc aatggccatg cccatggctt tgcaaggagg cgcaccatgc cgaaccatca
    15841 accccagggt gcggcgctgc gcaccgtggt gattctgggt gcgggtcaga tcggccaggc
    15901 gatcgcactc aagttgctcg atgccggggg ctatgcgctg ctggtggccg atcgcaatgc
    15961 tgcctcgctg caacgcttgc atgccttgac cggcataccc acggcacaga tccaggacgc
    16021 ggtggcgctg gaagccgtga ttgccggcgc ctacgccgtg ctcaacgcct tgccgtttca
    16081 ccaggccgtg ccggtggcca cgctatgcgt gaggcatggc gtgcattact tcgacctgac
    16141 cgaggacgtg gccagcacgc aagccatacg caacctggcg cgcgacgcgc gcagcgtgct
    16201 gatgccgcag tgcggtctgg cgccgggttt catcggggtt gtgggcaacg atctggccgg
    16261 gcggctggat gtggcgcggt cgctgcgcct gcgcgtgggg gcgctgccgc gctatccgca
    16321 gggcagcctg cgctacagcc tgacctggag caccgagggc ctgatcaacg aatactgcaa
    16381 gccctgcgag gtcattgccc aaggccggcg cagcaccgta ccggcactgg acggcctgga
    16441 gccgctgctg atcgacggcg tggagtacga ggcctttcac acctcgggcg gcctgggcac
    16501 gctgacgcag acctgggacg ggcgtatcga gcagctggac tacaagacca tccgctaccc
    16561 aggccaccgc gccgccatgc agctgctgct gaccgagttg cgcctgagcg agcgtcgcga
    16621 gctgctcaag gagctgctgg aaggggcgat tgccggcacc cagcaggatg tgatcgtcat
    16681 cctggccagt gccatgggcc agcgcgcagg gcgcctggtg caggagagct atgccgcccg
    16741 catcaccggc acgcggctgc gcggcacaac cctgagcgcc atacagcaca ccacggcggc
    16801 cagcatttgc acggcgctcg acttggtatc tacccagcga ctgccgcagg caggctttgt
    16861 cggccaggag cagatggcgt tgaccgactt tctggcaaac cgttttggct gtatttacga
    16921 cgccggggcc agccaggcct gatgctcctc aggccgcggc cttcagcccc tgggcgaaat
    16981 ggtcgcgcat ggcggccacg gtggcccgtg cgtcatgggc gcgcagcgcc gccagaatca
    17041 cctcgtgctc cgccagggag tcttctatgc gcccctgctt gagcagggag ttgtgtcggt
    17101 tgagcttcat gaccttgcgc agatccgcca ccatctggct gcgccaacgg ttgtcggcca
    17161 tctccagcat gcgcatgtgg atgcgctcgt tgatggcaaa gaagcgctcg cgctcgtcca
    17221 gggcggcggc caggtcggcg tgcagctgct ccagctcgcg cagctgctcg ggggtcgccc
    17281 gcgcggccac cacgccggcg gcgtcgctct ccagcagcgc cagcaggtgg tacacgtcgc
    17341 gcaaatcctt gtcgttcatc tcggtgacgt aggcgccgcg gcgcaccttc atggtcacca
    17401 gcccctcggc cgccagtacc ttcagggcct cgcgcagcgg cgtgcggctg atgccgaatt
    17461 cctcggcgat cttcagctcg tcaatccacc cgcccggctc cagctcgcgc cggaagatgc
    17521 gctggcgcaa ctgctccgcc acctgttcgt agagcgcgcg cggggtcagg gtgagggcgg
    17581 acatgggcaa atggtagcgc aaaaagagtt aagaattaat aattacagtt cgggtaaact
    17641 cgggaaaacc tgcgaaaagc agcgagtgat cctgcccctt tttgccatct ttgcgaaagc
    17701 accgcgccac catgagctct gacaatccca ctttccagcc cgccagcctt gacgcttggg
    17761 tccgcgccgc cgccaagtcc gctcctggcg gcgatgtgaa cgcgctcaac tgggtgacgc
    17821 ccgacggcat cagcgtcaag ccgctgtaca ccgccgctga tacgcaggat cttccctacg
    17881 ccaacacgct gccggggttt gagccctatc tgcgcggccc gcaggcgacg atgtattccg
    17941 tgcggccgtg gacgattcgc caatacgccg gcttttccac cgctgaagag tcgaatgcgt
    18001 tttatcgcaa ggcgctggcc gccggcgggc agggcgtgtc ggtggccttc gacctggcca
    18061 cgcaccgtgg ctacgacagc gaccacccgc gcgtcacggg cgacgtgggc aaggccgggg
    18121 tggcgatcga ctcggtcgag gacatgaaga ttttgttcaa cgagattccg ctggacaagg
    18181 tgagcgtctc catgaccatg aacggtgcgg tgctgccggt gctggcgggc tacgtggtgg
    18241 cggcggaaga gcagggcgtg tcgcaggaaa agctctcggg aaccattcag aacgacattc
    18301 tgaaagagtt catggtgcgc aacacctaca tctacccgcc caagccgtcg atgcgcatca
    18361 tcggcgacat catcgagtac acggccaaga atatgccgaa gttcaactcg atcagcatca
    18421 gcggctacca catgcaggag gccggggcga accaggcgct ggaactggcc tttaccctgg
    18481 ccgacggcaa ggagtatgtg aagaccgcca tcgccaaagg catggacgtg gacgagttcg
    18541 ccggacgcct gtccttcttc tgggcgattg gcatgaactt ctacctggag atcgccaaga
    18601 tgcgcgccgc gcgcctactg tggtgccgca tcatgaaggg ttttgacgcc aaaaacccca
    18661 agagcctgat gctgcgcacg cattgccaga catcgggctg gagcctgacc gagcaggacc
    18721 cgtacaacaa cgtggtgagg acgacgatcg aggccatggc cgccgtcttt ggcggcacgc
    18781 agtccctgca caccaacgcg ctcgacgagg cgattgccct gccgaccgag ttttctgccc
    18841 gcatcgcccg caatacgcag ctgatcatcc aggaagagac acacatcacc aacgtcatcg
    18901 acccctgggc cggctcctac atgatggaga agctgacgca ggacatggcg gatgccgcct
    18961 ggaagatcat cgaagaggtc gaaggcatgg gcggcatgac ggccgccgtc gatagcggct
    19021 gggccaagct caagatcgag gcggcggcgg cgcagaagca ggcgctgatc gactctggca
    19081 aggaggtcat cgtcggcgtc aacaagtaca agctggccaa agaagacccg gtggacattc
    19141 tggaagtgga caacgtcaag gtgcgggaca gccagattgc gcgcctgaac agcatcaagc
    19201 aaaagcgcga tgcagcccag gtgcagcaag cgctggcagc tcttacttct gcagcagaaa
    19261 gcggcaccgg caacctgctc gacctggcga tcaaggccat ccgcctgcgc gccaccgtgg
    19321 gcgaagtctc ggacgccctg gaaaagtctt ttggccgcca ccgcgcggat acgcaaatgg
    19381 tcactggtgt ctatgcccag gcctacgaag gcgccgaggg ctgggaaaag ttgaagggcg
    19441 agatcaacgc ctttgccgaa caacaaggcc gccgcccgcg cgtgatgatc gccaaactgg
    19501 gccaggacgg ccacgaccgc ggcgccaagg tggtgtccac ggcgtttgcc gacctgggct
    19561 ttgacgtgga catcggcccg ctgttccaga cccccgagga atgcgcgcgc caggcgatag
    19621 agaacgacgt gcacgcgatc ggcgtcagca cgctcgccgc tggccacaag accttggtgc
    19681 cggccatcat caatgcgctc aaggcccagg gggcagacga catcatcgtc ttcgtcggcg
    19741 gcgtgatccc ggcgcaggac tacgactttt tgaacgccgc gggcgtcaag ggcatctttg
    19801 gcccgggcac gccgattcca gccagcgcta aaaccgtgct ggagcagatc aaactggcta
    19861 tttccgcttg attggattac agtaatactg tattcaaagg agagttgtca tggctgtttc
    19921 ggtacgtttg gatccgctgc ttgaaaaaga cctggagctg gccgcaaaac gcctgggcct
    19981 gaccaagtcg cagttcattg tgaatgcgct ggaaaaggcc ttggagcgca aaaatccgta
    20041 tgaactgctg ctcaaggtcg agcgggaaat gcaggctgat ccgcattacg cacgcttgga
    20101 tgcggcattc gcaggcgagc cgcaaccggc ctatgacacc gagcaatcgc gcgcggcgct
    20161 gatcgccaaa ctccgaacta aacatggcct cagccctgat tgacgccagc gcgttggtgg
    20221 cggtgtttgg aaaaaaccag ccccatgccg agcattaccg agagctgctg accctggcct
    20281 cgctggagcg ctgggcgctg tccaccacct ggccttgcgt gattgaggct gcctatttgg
    20341 tcaaggcgcc gcagtgcttt gccctgttgc agtggatagg cgagggtgcg gtgatggtct
    20401 atccctttga tcagcggcat ttgctggact ttgtcaccgt gatgcagcgc tacaccgaaa
    20461 gcccgcgcac tgaaatggat ttggccgatg cctcgctcta ccacctggcg atcgaaaccg
    20521 gtgctacgcg catcctgacg ctggatcggc gcgacttttc ccgctaccgc ctgcccgacg
    20581 gccgcgcctt cgagattttg taaacccatg gatcaccctc tgctcgaccc gctgctgcac
    20641 ggcgcgccca tggcgcagcg ccgcgccatg gccaagacca tcacgctgct ggagtccacc
    20701 cgccaggacc accgcacgca gggcgaccag ctcctcacgg ccctgctgcc gcacaccggc
    20761 cgcgccctgc gcttaggcat cagcggcgtg cccggcgtgg gcaagtccac cttcatcgag
    20821 gcgctgggcc tgtacctggt gcgccagggg ctgaaggtgg cggtgctggc gatcgacccg
    20881 tcatcctccg tcaccggcgg ctccatcctg ggcgacaaga cgcgcatgga gcagttgtgc
    20941 gcgcaggaag ccgcctacat ccgccccagc cccagcagcg gcacgctggg cggcgtcgca
    21001 gagaaaacgc gcgaggccat gctggtgtgc gaggccgcag gctacgacgt ggtgattgtc
    21061 gagactgtgg gcgtgggcca gagcgagacg gcggtgcacg gcatgaccga catgttctgc
    21121 gtgctgcagc tgcccaatgc gggcgacgac ctgcaggcca tcaagaaggg cgtgatggag
    21181 ctggccgacc tggtggtcat caacaaggcc gacatcgacc cggccgccgc cacccgcgcc
    21241 caggcgcaga tcacgtcgag cctgcgcctc ttgggctttc acggcaaccc cgagcacgac
    21301 ccgcacaacg cccagcaatg gacgcccaag gtattgcaga tcagcgcctt gcagcagcag
    21361 ggcgtcgacg gcttctgggc cgccgtgcag gactaccgcc agatgcagag cgccaacggc
    21421 cgcctggccg cgcggcgcga aaagcaggcc ctggcctgga tgtgggagcg catcgacttc
    21481 ggcctgaagc aggcttttcg tgcccatccg caggtgcagg cgctgctgcc gcagatgcaa
    21541 gccgatgtgg ccgccggccg catcgccgcc agtactgcgg caagaaatct gcttgcagcg
    21601 catatgggat aagcgctaca agctatcaag tttatagtta tcagattcca acaaaggaca
    21661 gaaccgatgc aaaccatcct cgaccaactg gagaaaaagc gcgaactcgc ccgcctgggc
    21721 ggcggccaaa agcgcattga cgcgcagcac gccaagggca agctcacggc gcgcgagcgc
    21781 atcgaggtgc tgctggacgt cggctcattc gaagagtggg acatgttcgt cgagcaccgc
    21841 tgtgcggact tcggcatgga tgccacgcat attcccggcg acggcgtggt gacgggctac
    21901 ggcaccatca acgggcgcct ggtgttcgtc ttcagccagg acttcacggt gttcggcggc
    21961 gcgctgtccg aggcgcatgc cgagaagatc tgcaaggtga tggatcaggc catgaaggtc
    22021 ggtgctccgg tcatcggtct gaacgactcg ggcggcgcgc gcatccagga gggcgtggcc
    22081 agcctgggcg gctatgccga ggtgttccag cgcaacgtca tggccagcgg cgtcattccg
    22141 cagatcagca tgatcatggg cccgtgcgcc ggcggcgcgg tgtacagccc ggctatgacc
    22201 gacttcatct tcatggtcaa ggacaccagc tacatgttcg tcaccggccc cgaagtggtc
    22261 aagaccgtca cgcacgagag cgtgacggcc gaggaactgg gtggcgcgct cacgcacaac
    22321 accaagagcg gcgtggccga cctggctttc gagaacgacg tcgaggccct cttgatgctg
    22381 cgccgcttct tcaactacat ccccgccagc aaccgcgaaa aggcgccgca ccgccccacg
    22441 caggaccgcg tcgaccgcgc cgacctgagc ctggacaccc tggtgcccga gaaccccaac
    22501 cagccctacg acatcaagga ggccatcgtc aagacggtgg acgacggcga attcttcgag
    22561 ctgcagcccg actacgccgc caacatcgtc atcggcatgg cgcgcatgga cggccatgtg
    22621 gtgggcgtgg tggccaacca gccgctggtg ctggccggct gcctggacat caagagcagc
    22681 atcaaggccg cgcgcttcgt gcgcttctgc gacgccttca acatccccgt catcaccttt
    22741 gtcgacgtgc ccggcttcat gcccggcact gcgcaagagt acggcggcat catcaagcat
    22801 ggcgccaaac tgctctacgc ctacgccgag tgcacggtgc ccaagatcac cgtcatcacc
    22861 cgcaaggcct acggcggcgc ctacgacgtg atgagctcca agcacctgcg tggcgacgtg
    22921 aatctggcct ggccgaatgc cgagatcgcg gtgatgggcg ccaagggcgc ggtcgagatc
    22981 atcttccgcg aggaaaagaa agaccccgcc aagctggccg cgcgcgaggc cgaatacaag
    23041 gcccgctttg ccaacccctt cgtcgccggc agccgcggct atatcgacga cgtaatccag
    23101 ccgcacgaga cgcgccagcg catctgccgc agcctggcca tgctcaagga caagaagctg
    23161 gacaacccgt ggcgcaagca cgggaatatt ccgctctgat ttgaatagct gatcacgcaa
    23221 tgcccaaggc tgccaccgcc caacaggctg tcaaactgac cgatataccc aacatcggcc
    23281 cgtcgattgc cgccgatttg aaggggcttg gcgtgcatgc cccaaccgac gtaggacgca
    23341 tggacccttg gcatacctac gacgcattgc gtgggcccat gggccagcgc catgacccct
    23401 gcgtgctcga cgtgttgttg gccgcgcacg agttcatgaa tggtggcgcg gccaagccct
    23461 ggtgggcgtt cacggcgcag cgcaaagccc agtgggcgtc gcggttcaag accgcagcga
    23521 aaggcttgaa gtgatggtct ggctagtgca agcccaacac ggcggcgagc gcttcgtcca
    23581 gcagaagcag cgcatcggca tccagggtgt cgatgcgctt gcgcagtcgc gatttatcca
    23641 gcacgcgcac ctgttcaggg cgagcgcggg tcttggagct gaagctgggc agggcaatca
    23701 gcaagggcca aacaggctcg gccgtggtgg tggtgagcgg cacgatgaca acggtgcgcc
    23761 ggatcgcgtt gaactgcgtc gccgagacaa cgacgcaggg tcgagtcttg gccatctcgg
    23821 agccgcgcac cgggtcaagg tcggcccagt acacgttgcc gcgttcgatc ttcatcgctt
    23881 ggccgggaga gccgattggt ttcgggtttt ctgggcttca gccagatcgc gctcaaacat
    23941 ctcgcgatca aagtcggcgt cccagacatc gaccgagtcg gtcaactcgg cggcttccca
    24001 ttcacgggct tcctgcagca actctggatg cttggcttcc agttcgttca tgcggttggc
    24061 tgcatcgatc aacacctggt ttcgggcctc ttcttgctcg gccaagcctt tggcaagcag
    24121 atccaccaga aaccggttgc gctggcgagg tgccacggcg cgagccagct tggcggccag
    24181 gtcttcgggc aggcgcagca gcacctggcg gggtggggcg ttcaacgtat gcatcatgat
    24241 gtgatgatat caagttatca aaatttaagt tactgagaaa gagatcacta tgttcaccaa
    24301 aatcctgata gccaaccgcg gcgagatcgc ctgccgcatc atcaccaccg ccaagcgcat
    24361 gggcattgcc accgtggcgg tgtattccga cgccgacaag aacgcgcgct tcgtgcagct
    24421 ggccgacgag gccgtgcgcc tgggcccgcc gccgtcgcgc gagtcctacc tgctggccga
    24481 caagatcatc gaggcctgca agcagaccgg cgctcaggcc gtgcatccgg gctatggctt
    24541 tctgtccgag aacgccgagt tcgccaagaa ggtggaagag cagggcatcg ccttcatcgg
    24601 ccccaagcac gctgccattg ccgccatggg tgacaagatc gcctccaaga agctggcggg
    24661 cgaggccagg gtcagcacca tccccggcta caacgacgcg atagaaacgc ccgagaaggc
    24721 ggtggaaatt gccaagggca ttggctatcc ggtgatgatc aaggccagcg cgggcggcgg
    24781 cggcaagggc ctgcgcgtgg ccttcaatga caaggaggcc tatgaaggct ttgcgtcctg
    24841 caagcacgag gcgctgagcg cctttggcga cgaccgcgtg ttcatcgaaa aattcgtcga
    24901 gcagccgcgc catatcgaga tccaggtgct gggcgacgcg catggcaacg tggtctacct
    24961 gaatgagcgc gaatgctcca tccagcggcg ccaccagaag gtgatcgagg aagcgccttc
    25021 gcccttcatc agcgccgcca cgcgcaaggc catgggcgag caggcggtgc agctggccaa
    25081 ggccgtgggc taccagagcg cgggcacggt ggagttcgtg gtcggcaagg atcaggactt
    25141 ctacttcctg gagatgaaca cccgcctgca ggtcgagcac ccggtgaccg agttgatcac
    25201 cggcctggat ctggtcgagc agatgatccg cgtggcggcg ggcgagaagc tggccttcac
    25261 ccaagcggat gtcaagtgcg aaggctgggc catggagtgc cgcatcaatg ccgaagatcc
    25321 gttccgcggc ttcctgccct ccacggggcg cctggtgcgc ttcgtaccgc cggcgcagga
    25381 cctgcaccag ggcctgcctg ccacggggct gggcgtgcgc gtggacacgg gcgtgctgga
    25441 cggcggcgaa atcccgatgt actacgactc aatgatcgcc aagctgatcg tgcacggcaa
    25501 ggatcgtgcc gacgccattg cccgcatgcg cgaggcgctc aacggcttcg tcatcaaggg
    25561 cgtgcaaagc aacatcccgt tccaggccgc gctgctggcg cacccggatt tcgtcagcgg
    25621 caacttcaac accggcttca tcgccgacca ctatgcacat ggctttcggg ccgaggatgt
    25681 gccgcacgaa gacccgtcgt tcctgctggc gctggcggcg catctgcaca tgttctaccg
    25741 caaccgcgcc agcgggctgc agggccagtt ctgggagggc ttcaagcgcc tgccgcggcg
    25801 cgagttctcg gtatgcgtgc tgggcgaggg cgggcacaac agctacaccc gggccaccgt
    25861 ggccgaaacc gagcccggcc acgccacgcc cgtgaccctg cacctgccgg gtggcgacaa
    25921 gacctaccac ctgagcgtcg atcgccacct gggcgaggtg cgcatcaccg gcacggtgga
    25981 tggcaaggcc ttcaccgcgc aggtcgagcg cggcaagacg agcaacccgc tggccatccg
    26041 tgtgatccac aacggcacac gcctggacgc catcgtcatg cacccgcgca ccgccgatct
    26101 gcaccgcctg atgccctaca aggcgccgcc ggacttgagc aagttcgtgc tctcgcccat
    26161 gcccggcctg ctggtgcagg tggcggtgca accggggcaa gaggtcaagg ccggcgagcg
    26221 cgtggccgtg atcgaggcca tgaagatgga gaacgtgctg ctggccagcg ccgacggcgt
    26281 ggtcaaggaa atcaaggcgc aaaagggcga cagcctggcg gtggatcagg ccatcgtcga
    26341 gttcgagtga gcgtggcggg ttgaacgccc gcgcagaggg tttggagtgc gctgtgccac
    26401 cttgctggcc aacgacggcg tgaatccatg cccccgtcaa cgcgggcatg gctgcacgtc
    26461 tgatttatga agagaaaaat cgctgtgacg cttatgtggc aagcgctggc agctattaaa
    26521 tcaatagtta aagcgaaaaa ggaagccaat ccatgacacg ccccttcaaa gtcctgggca
    26581 tccagcaggt cgccatcggc ggcaccgaca agggccgcat gaaaaagctc tgggtggaca
    26641 tgctgggtct tgagcaaacc ggcagcttcc agagcgagcg cgagaacgtg gacgaggaca
    26701 tcctggccat ggggcgcggc gcgcacaagg tcgaggtgga catcatgcag ccgctcgaca
    26761 tcgacaagaa gccggcggtg cacaccacgc cgctgaacca tatcggcctg tggatagacg
    26821 acctgcccaa ggcggtggaa tggctcacgg cgcaaggcgt gcgctttgcg cccggcggca
    26881 tccgcaaggg cgcggctggc tacgacatca cctttttgca ccccaagagc aatgacgagt
    26941 tccccattgc tggtgagggc gtgctcatcg agctggtgca ggcgccgccc gaggtgatcg
    27001 ccgcgctggg ctgaaacacg aggcagcgcc agcgcggccg gccgggttct tacactcggc
    27061 gcatgtccac tgccgcaccc cgaccatccc cggccgcccc gcgcggccag ccgcgcgcgc
    27121 tgggcgcctt gctgcccttt ctgcggccct atcgctggcc gatcgccgct gcgcttgcct
    27181 ttctgctgct ggcggcactg gccacgctgg cctttccgct ggcgctgcgc cagctgatcg
    27241 accagggcgg cagcagccag gcggcggcgc tgcgcgataa ctttggcctg ctgtttgccg
    27301 tggccgtcgc cgtgggcctg ttctcggccg cgcgctacta cgccatgagc tggctgggcg
    27361 aacgcgtcac ggccgacctg cgcagccgcg tctacgccca tgtgctcacg caaagcccgg
    27421 ccttcttcga gaccacgcag accggcgagg tgctctcgcg cctgacggcc gacaccacct
    27481 tggtgcagac cgtcatcggc tcgtcgttct cgctgggcct gcgcaatgcc gtgctgggcc
    27541 tgggcgcgct ggccatgctg gtgtggacca accccgtggt gatgtcggcg gcgctggccg
    27601 ccgtgttgat ggttgtgctg cccaccatgt ggataggccg cagcgtgcgc cgcctgtcgc
    27661 gcgccagcca ggaccgcgtg gccgatgcca gcgccatcgc cgccgaggtg ctgggcgcca
    27721 ttcccatcgt gcagggcttc accgccgagc ggcgcgaggc ggcgcgcttt gccgcctcgg
    27781 ccgaggccgc cttccagacc gccgtgcgcc gcagcggcgt acgtgcgctg ctcgtggcct
    27841 tcatcatcat cgccaatgcc gcgctgctgc tgtggggtct gtaccgcggc agccaggcgg
    27901 tgctggccgg gcagatgagc gccggcagcc tgggccagac catcgtctac gcgctcttcc
    27961 tggccggcgc cgtggccgtg ctgggcgagg tctatggcga cctgctgcgc gcggccggcg
    28021 ccatggagcg gctgatggag ctgctggcca cgcggcccgc catccgctcg cccgaggccg
    28081 gcacgctgca agggctcgcc ccgggccagc cgctggccgt ggccttcgag gcggtggatt
    28141 ttcactaccc ctcgcgcccc gaccgtgcgg cgctggcggg cttcacgctg cacctggcgc
    28201 ccggcgagac ggtggcgctg gtgggcgcca gcggcgccgg caagtccacc gtgttccagc
    28261 tgctgcagcg cttctacgac gttcaggcag ggcgcatcct gctgggcggc caggacatcc
    28321 gcggcctcga tctgcacgcg ctgcgcgggc gcatcgccac cgtgccgcag gacgcggtga
    28381 ttttttccag cagcgcgctg gacaacatcc gctacgcacg ccccgaggcg acgcgcgaag
    28441 aagtcatcaa cgccgcacgt gccgccttcg cgcacgactt catcgaggca ctgccgcagg
    28501 gctacgacag cttcctcggc gagcatggcg tgcgcctgtc gggcggccag cgccagcgca
    28561 tcgccatcgc ccgcgcactg ctcaaggatg cgccggtgct gctgctggac gaagccacca
    28621 gcgccctgga cgcgcacagc gagcgcgtgg tgcagcaggc gctggacgcg gcactgcaaa
    28681 gccgcgccgg cagccgcatc accctggtca tcgcgcaccg cctggccacg gtgcagcatg
    28741 ccgaccgcat cgtggtgctg gaccacggct gcatcgtcga gcagggcagc catgccgcct
    28801 tgatggccgc agacggcgcc tacgcgcgcc tggcggcgct gcagttcacg gcgtgacggc
    28861 gggctcctgc gatatttctg gcttcatttc gccagcggtt tcgccagcat tcgccgcagt
    28921 tgccgcctga ttggccggca ttggcgacga atggtgtttg cgtcgtcatt tgccttcaaa
    28981 aatggccaat tggtctgctt ctgccgcaaa aaatgagcgc ttttgggctg ttttacaggc
    29041 agaaattaag tgccaactat ttgcggcgcg ggggcttcac agcgcgcaac ggcattcaca
    29101 caatcgcagc cgccctgcag cctggctgcg ttttggagtg atgcatgaca actgtttttc
    29161 tggccgatgc gccaaccccc gcaaaccatg cccggcccgc ccacgcgtcg cctgccgcgg
    29221 cgcagcgctg gagcgtacag gcggtgcagg agctgctgga tcgccccttc atggacttgc
    29281 tctggcaggc ccaggaggtg caccgccagc attggccggc gggtgacatc gaactggcaa
    29341 ccctgctgtc agtcaagacc ggcggctgcg ccgaaaactg cggctactgc ccgcagtcca
    29401 gtcatttcga cacgggggtg caggcgcaaa agctgatggc cgtggacgag gtgcgcagcg
    29461 ccgcccaggc ggccaaaaat gccggcgcca cgcgcttttg catgggcgcc gcctggcgcg
    29521 cgcccaagga gcgcgacatc gaaaaaatgt cggagctgat ccgcacggtg aagggtctgg
    29581 gcctgcagac ctgcgccact ctgggcatgc tgcagccgca ccaggcgcag gcgttgaaag
    29641 ccgccgggct ggactactac aaccacaacc tggataccgc ccccgagtac taccaggacg
    29701 tggtcagcac gcgcagctac caggatcgcc tggacaccct gcggcatgtg cgcgccgcgg
    29761 gcgtcaaggt gtgctgcggc ggcatcgtcg gcatgggcga ggcaccggtg caccgcgccg
    29821 ggctgatcgc gcagctggcc aacctcgacc cataccccga gtccgtgccc atcaacagcc
    29881 tggtgccggt gcccggtacg ccgctggcgg gcagcgcgcc ggttgattcg ctggattttg
    29941 tgcgcgtgat tgccgtagcg cgcatcacca tgccgcgcgc gcgcgtgcgc ctgtcggccg
    30001 ggcgcgagca gctgggcgag gcggtgcagg cgctgtgctt tctggccggg gccaactcca
    30061 tcttctacgg cgaaaagctc ttggtgaccg gcaaccccga tgtcgccgct gatgagcggc
    30121 tgttggccaa gctcggcctg cgcgcccatg ccacccatgg acaggcgcag ccggcgggga
    30181 ctgaccgtgg ttgtgcatgc tcctaaaata ggagctgttg gcgcttatgg tgtatgagat
    30241 aaggccaaaa aaaccataaa cttttgatcc gccttcaagc cgatcagcag cccggctgta
    30301 tgcccagctt gcgcagcatg gtctccagca ggcaccactt ggtcagcccg ctttgcagca
    30361 ggttggcgcc cacgaaagcc gtgaaggcca gccaccattg gctggcgaag atcgggctgc
    30421 cgggaatgcc cagcg
//
LOCUS       DAOMTQ010000012        30422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_124_length_30422_cov_26.479665, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000012 DAOMTQ010000000
VERSION     DAOMTQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 30422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30422
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124_length_30422_cov_26.479665"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK518_02420"
     CDS             <1..669
                     /locus_tag="PK518_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM47793.1"
                     /translation="LLRGIVAIAREHELVLFADEVYDKVLYDGVGHTAMASLSSDVLT
                     LTFNSLSKAYRSCGYRAGWMVVSGDKAAARDYIEGLNMLANIKLGSNVPGQWAIQTAL
                     GGYQSINDLVKEGGRLRRQRDLAYELITQIPGVTCVKPQAALYMFPRLDPDMYPIADD
                     RQFFMEVLRATRVMLVQGSGFNYPDNQHFRIVFLPHEDDLREAVARLAAFLATYRQRN
                     AKAA"
     gene            738..2072
                     /locus_tag="PK518_02425"
     CDS             738..2072
                     /locus_tag="PK518_02425"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM47794.1"
                     /translation="MKPIQVGLLGIGTVGSGVFNVLQRNQEEIRRRAGRGIEIACVAD
                     LDTARARSIVGEGTRVVGDAREVIANPEIDIVIELIGGYGIAKQLVLEAIAAGKHVVT
                     ANKALLAVHGTEIFKAAADKGVVVAYEAAVAGGIPIIKALREGLSANQIQWVAGIING
                     TTNFILSEMRDKGLDFDVVLKEAQRLGYAEADPTFDIEGVDAAHKATIMSAIAFGIPV
                     QFDKAYVEGITKLSAADIRYAEQLGYRIKLLGITKRTDKGVELRVHPSLVPAKRLIAN
                     VEGAMNAVVVHGDAVGTTLYYGKGAGSEPTASAVIADLVDITRLIEADPAHRVPPLAF
                     QNHTLGAAGQDLPVLPMAEVVTSYYLRIRVADQAGVLARITGLLADAGISIDAVLQRE
                     ADEVGGEGSTQTDVIILTHDTREGDMDGALAQIQALPTVLAPITRIRKEELN"
     gene            2134..2397
                     /locus_tag="PK518_02430"
     CDS             2134..2397
                     /locus_tag="PK518_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM47795.1"
                     /translation="MSAVWQVQEAKNHFSEVIDRALTEGPQTVTRHGKPVVRVVAVTE
                     GDMQEPADDGFLEFLLSAPKVEGGLPEMPRDTNPGRSPMFGED"
     gene            2404..2832
                     /locus_tag="PK518_02435"
     CDS             2404..2832
                     /locus_tag="PK518_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRM47796.1"
                     /translation="MRWLLDTCTISESTYPQPHAGVLDWLRRHHHDCAIAAASFTEIH
                     YGIACLPHGAKRNRLQAWALELAQQFDGRILASDDAVWRQFGDLKASLRAMGRMQDAL
                     DMVIAATALHHGLPLVTRNTRYFEDTGLRLVNPWADVSQA"
     gene            2841..4271
                     /gene="thrC"
                     /locus_tag="PK518_02440"
     CDS             2841..4271
                     /gene="thrC"
                     /locus_tag="PK518_02440"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRM47797.1"
                     /translation="MQYLSTRGHPERKRFCDILLEGLAPDGGLYLPETYPQIDDAALT
                     QLRRAYNEEGYAALAFRILSLYIDDIPAADLKALCAKTYTQEVFGTKEIVPLRQLEDG
                     LWIEALSNGPTLAFKDMAMQLLGNLFEYELARRGEELNILGATSGDTGSAAEYAMRGK
                     QGVRVFMTSPHGRMSPFQQAQMFSLQDENIHNLAIEGVFDDCQDIVKAVSNDHAFKAR
                     YKIGTVNSINWARLLAQVVYYFAGYIQATQGNEQKVSFTVPSGNFGNICAGHVARQMG
                     LPIDRLVVATNENDVLDEFFRTGVYQVRASEHTYETSSPSMDISKASNFERFVFDLVG
                     RDGARTQALFADGVGRAGRFDLSQDAVFQDTAGKYGFVSGKSTHADRLATIRDTYQRF
                     GQIIDTHTADGVKVARERRGDLAVPMIVLETALPIKFAETIQEALGRSPERPARFEGI
                     ELLPKRVQQLPADVERVKAYIAAHCD"
     gene            4342..4893
                     /gene="mobB"
                     /locus_tag="PK518_02445"
     CDS             4342..4893
                     /gene="mobB"
                     /locus_tag="PK518_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRM47798.1"
                     /translation="MKVLGFAGYSGSGKTTLLEQLIALLRLRGLRVSVVKHAHHRFDI
                     DHQGKDSWRHREAGAYEVVVASDRRMALMREYEQPHAIDPHELLAELDPRVDWVLVEG
                     FKHSDLPKVEVWRPAAPGQPERPVHYGGDDHVVAVATDAAQLPRPTALPVLNLRDPEA
                     VAQWMLEQGARFDYLPQAVEACE"
     gene            4890..6119
                     /locus_tag="PK518_02450"
     CDS             4890..6119
                     /locus_tag="PK518_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRM47799.1"
                     /translation="MTARTPLIPLDDALTELLAQARPLAGVDTVSTFDADGRVLAQAA
                     ISPLQVPQQDNSAMDGYALRCADVAAAGVELRVSQRIAAGDSGQPLLPGTAARIFTGA
                     PVPAGADAVVMQEDCEALDDGQRVRINHLPSPGQAIRRAGEDITLGAQVLAAGTRLTP
                     AELGLAASIGLAQLQVARRPRVALFSTGDELVMPGEVAPAQMRPGAIYNSNRFFLRAM
                     LQRLGCAVTDMGVVPDRLDATVAALREAAQGHDLILTSGGVSVGEEDHVKPAVQSLGS
                     LDLWQIAIKPGKPFAYGRIGGAHFIGLPGNPVSSFITFALLVRPFLLRLQGVVNVAPT
                     TIAASAGFDWPRVDKRREFLRARHGADGRLELFANQGSGVLTSVAWGDGLVDNPAGHA
                     IARGDQVRFLPFAELLS"
     gene            6116..6379
                     /locus_tag="PK518_02455"
     CDS             6116..6379
                     /locus_tag="PK518_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRM47800.1"
                     /translation="MTAPLTITVRYFASIREAVGQGQETLRTAAPTLGALRDELIARG
                     QPWADCLARGRAVRMALDQTMAQEATPLADGAEAAFFPPVTGG"
     gene            complement(6383..6703)
                     /locus_tag="PK518_02460"
     CDS             complement(6383..6703)
                     /locus_tag="PK518_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47801.1"
                     /translation="MHDDNQILIPPSFIALHSDARQRLLLPVDELRARYELCEDLAQH
                     LVEQAQQLYHGGARSEEGVLLGMHAGLASPDAGVSAPEARWITLRLAELLGWRSPALP
                     TTGD"
     gene            complement(6756..6899)
                     /locus_tag="PK518_02465"
     CDS             complement(6756..6899)
                     /locus_tag="PK518_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HRM47802.1"
                     /translation="MQLLKQLFTTEVGLFSAVGIGFMLCMLVFFVWLFTRNDAPPADQ
                     KQP"
     gene            complement(7036..7290)
                     /locus_tag="PK518_02470"
     CDS             complement(7036..7290)
                     /locus_tag="PK518_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47803.1"
                     /translation="MALQTINTDDYTLYPSPRNQHRVVFEFQTFVPHPYALIDLPSFE
                     LAGRHSLFAAHRRADNKMGQLVTFELASDQQRFERLFVPD"
     gene            7337..7813
                     /locus_tag="PK518_02475"
     CDS             7337..7813
                     /locus_tag="PK518_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRM47804.1"
                     /translation="MTRVSIQTQDFDVSAELARLRAQDARVGAVCCFVGTVRDRNDGD
                     AVATMELEHYPGMTEKSIEAMIAAALARFDLYGVRVIHRVGLLAPLDQIVLVAVTSAH
                     RGESFQACEFLMDYLKTQAPFWKKEETPQGARWVDARVADDAALARWGIAAPAQFD"
     gene            7882..10485
                     /gene="clpB"
                     /locus_tag="PK518_02480"
     CDS             7882..10485
                     /gene="clpB"
                     /locus_tag="PK518_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRM47805.1"
                     /translation="MRFDKFTTKFQEALSDAQSIALGHDNAYIEPVHMLVAMLRQDEG
                     PRALLERAGANAPGLLKAAEAAVKRLPQVQGQDQVQGSPELGRVLQATEKEAIQRGDQ
                     FIASELFLLALIDSKGEAAQIAKDNGLSRKSLEAAIDAVRGGQKVDSAEAEGQREALK
                     KYCLDLTERARLGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLA
                     QRIVEGEVPESLKNKRVLVLDMAGLLAGAKYRGEFEERLKSVLKEVAHEEGRIILFID
                     EIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQKV
                     LVQEPSVEDTIAILRGLQVRYEAHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLI
                     DEAAAKIKIEIDSKPEALDRLERRMIQLKIEREAMKKEHDEASQKRLALIEEELANVE
                     REYADLEEIWKMEKASAQGSEQIRKDIDAIRTQMDDLKRKGDFNKVAELQYGRLPELE
                     KRLKEAQDSEADGETPKHRLLRTHVGAEEIAEVVSRATGIPVAKMMQGEKDKLLHMED
                     KLHERVVGQDEAISAVANAIRRSRSGLSDPNRPLGSFLFLGPTGVGKTELCKALAGFL
                     FDSEEHLVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVLLLDEVE
                     KAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSPLIQAMVGQDADDIKE
                     AVWGELKNHFRPEFLNRIDETVVFHGLDARHIEAIARIQLHLLQARLAKMDLQLEVSP
                     AAMGELAKVGFDPVFGARPLKRAIQQRIENPLSKLLLQGHYPPKSVIPVDVDPVQEPG
                     VFHFGQARQLA"
     gene            10553..10855
                     /locus_tag="PK518_02485"
     CDS             10553..10855
                     /locus_tag="PK518_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47806.1"
                     /translation="MNDVVTGVIRWVLRLVVLAMGVLFFLSLLAAAAVLALVWGLRAL
                     WARLTGRPVTPWVMRMDPRTGWSTVYRSTARWTAQRPEPPRRDDHDVTDVVPREIR"
     gene            10911..12356
                     /locus_tag="PK518_02490"
     CDS             10911..12356
                     /locus_tag="PK518_02490"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRM47807.1"
                     /translation="MRATLPDDIRHVFELQRQASRAQPDTPLTLRRERLLRLSKLIDE
                     HGPALAAAVQADFGMRSPRLTEVADLFMLRAQLAHTLRHLARWCRRTRVRTPLFLLPA
                     RGWIERQPLGVVGVISPWNYPVQLALAPVIGALAAGNRVMLKPSELTPHTAAQLASLV
                     AQFFAPDEFCVVQGDAATAALVASLPFDHLVFTGSTAVGRKVAQAAAANLTPTTLELG
                     GKSPAIIDTDCDLREAALKIVHGKLLNAGQTCIAPDYVMLPRGSEAAFAEAYRAAVAR
                     LFPYFEGNPDYAAIVSPRHLARLRTMLQQAQTLGAELQVLQSVPGSPPPPPQGIGDGV
                     GRQMAPVLVFGATPAMQLMQEEIFGPVLPVLSYERLDDAIAHINAGPRPLALYWFGRG
                     EAARDDVLRRTVSGGVCVNDTLLHVAHDNLPFGGVGDSGWGAYHGEAGFLRFSHQKSV
                     LAQSRWAQGHLLYPPYGARFDRVMALLRRLV"
     gene            12513..13034
                     /locus_tag="PK518_02495"
     CDS             12513..13034
                     /locus_tag="PK518_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM47808.1"
                     /translation="MSSIQVRPATLRDAKAIAQVHTTAALEAYRGLVPDDQLKSMSSV
                     EKRQAYWREAIEYCEPQVQVAVDGDKIVGFVGFDRSRDEKTRNTTGEIWAIYAAPTHW
                     DKGVGVALWDAARDGLQEEGCTTVTAWVPLRNERALRFHEMAGFKREMTTAKTALVGG
                     VKIEEVRLKRPVN"
     gene            13057..14208
                     /locus_tag="PK518_02500"
     CDS             13057..14208
                     /locus_tag="PK518_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM47809.1"
                     /translation="MIEWSEQGEAQRARWHSESGAAAPARVQPADDTLSADSAYRLAC
                     EGTALLWRGDFQNARQLLQALMRRADRKNPKAAAKAAQKLAGATPSERFHLHRQAQAQ
                     RARTLGALLIPVAGDYSIPLRRAPDWREACTEAWGPADGTPSVVALRELLGMVGAHEW
                     RKKGVEIPALGAAPGNRIHPHYGVFSPLRGEYIDLVAQALLPSTELAFDIGTGTGVLA
                     AVLARRGVRRIVATELAPRALVCAQDNLQRLGLQGRVELLAADLFPPARAPLVVCNPP
                     WLPARPSSAVEQAVYDEGSRMLKGFLAGLAAHLSPGGEGWLILSDLAEHLGLRSRDEL
                     LGWIAAAGLRVLGRQDTRPRHSKAQDAGDMLHAARAAEVTSLWRLAVAQ"
     gene            14305..15237
                     /locus_tag="PK518_02505"
     CDS             14305..15237
                     /locus_tag="PK518_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRM47810.1"
                     /translation="MEGLLDFVLRDVLTRVGGVDRCVSEFIRVSGNLLTDRVFLSYIP
                     ELKNGGRTLAGVPVRAQLLGSDPGCMAENAARLAALGPEGIDLNFGCPAKTVNRHGGG
                     AALLREPESIAAVVAAVRRAVPAPLPVSAKMRLGYDDAGLARECAQAMEQGGATELVV
                     HARTKADGYRPPAYWEQIPAIRAAVAIPVVANGEIWSVADALRCRSLSGCDALMLGRG
                     MVADPGLALAIRAHDQGQAHQPLAWRALLPQIGRFWDLVCADLEPRQRAGRLKQWLNL
                     LRRCYPEAQRAFDEVRVMTDQRVITAWVRQVADQ"
     gene            complement(15231..16037)
                     /locus_tag="PK518_02510"
     CDS             complement(15231..16037)
                     /locus_tag="PK518_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47811.1"
                     /translation="MNNTGYSRFLQEQWPQQQPLVARYMLAGEQVWLKRAGPRHGMWR
                     YRLLGAAAGVLRLPVLRPVPNLGGRPAIATELRRLRTLGALGLRVPQVLAACDDAFLM
                     RDLGTPGRPTPSLGDEIEAAVAAGPTAVLALWRLGLQTLDAVHGHQQCLSQAFARNMV
                     RCPDGAIACIDFEDDPLSALPLALCQLRDALAYAHSTALALRQAGAQQEARALWNDWL
                     TQPLRGADFQAALQGTLTRLTWLRHLPQDRRWGRDVQRLRAAHDLLIGSY"
     gene            complement(16034..17146)
                     /gene="ribD"
                     /locus_tag="PK518_02515"
     CDS             complement(16034..17146)
                     /gene="ribD"
                     /locus_tag="PK518_02515"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRM47812.1"
                     /translation="MTGATPFIQQALGLAAQALCLSNPNPRVGCVIAGGDGAVLGQGF
                     TQQAGGPHAEVVALRDAAARGHDVRGATAYVTLEPCAHQGRTGPCCDALIQAGIGRVV
                     ASIADPNPQVAGQGFARLRAAGVEVEVGPGAAQARELNIGFFSRMQRGTPWVRMKAAA
                     SLDGVTALANGASQWITAPAARADGHAWRARACAVLTGIGTVLDDDPLLNVREVATPR
                     QPHLVVVDSRLQTPPTARLFGVPDRRVFIYAAHEDGTRKQALEARGATVIQLPNAHGK
                     VDLPALLRDLGPRGVNELHLEAGHKLNGSFVREGLVDEMLLYLAPLLLGSGAHGLASW
                     GPLQALAQGLRMEFHDIQQIGPDLRLIARVAGRAQF"
     gene            complement(17272..17347)
                     /locus_tag="PK518_02520"
     tRNA            complement(17272..17347)
                     /locus_tag="PK518_02520"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17312..17314),aa:Lys,seq:ctt)
     gene            complement(17431..18408)
                     /locus_tag="PK518_02525"
     CDS             complement(17431..18408)
                     /locus_tag="PK518_02525"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HRM47813.1"
                     /translation="MEIFDYDNILLLPRKCRVQSRSECDASVELGGRRFKLPVVPANM
                     KTVVDEKICAWLAQNGYFYVMHRFDLDNVRFVRDMRAQGCFASISLGIKQADYDTVDR
                     LKAEGLTPEYITIDIAHGHSDSVRDMIGYLKKHLPQSFVIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITKLKTGFGTGGWQLSAMKWCARVATKPIIADGGIRSHGDIAKSI
                     RFGATMVMIGSLFAGHEESPGHTVEVDGELYKEYYGSASDFNKGEYRHVEGKRILEPI
                     KGKLADTLTEMEQDIQSSISYSGGKKLMDIRKVNYVILGGDNAGEHLLM"
     gene            complement(18613..19299)
                     /locus_tag="PK518_02530"
     CDS             complement(18613..19299)
                     /locus_tag="PK518_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47814.1"
                     /translation="MKTPALIAAGLCALPLAGALAQPCSSDHTPRPAALFERFVSADC
                     ATCWSEPHTPAPGPSALVVDWIVPTPSGDDAPLSSAATADALARLQALARAAPAGTDV
                     HTDSLITAPGSLRVAMGPAVNDYVGTIIRFSPPPKARPPLRFWLVLVEQLPAGAEASP
                     VARHVVRNAWHGEWPGGALSDLRPMRIPDGADVERLGLLGWVQDAAGRTVAAAQALCA
                     GQPVPLPRPE"
     gene            19334..21745
                     /locus_tag="PK518_02535"
     CDS             19334..21745
                     /locus_tag="PK518_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM47815.1"
                     /translation="MLPQDSLSGAGVPPSGQAAPLLRGLNPEQLAAVTLPAGHALILA
                     GAGSGKTRVLTTRIAWLLSTGQATPGGILAVTFTNKAAKEMLTRLSAMLPINVRGMWI
                     GTFHGLCNRLLRAHHKLAGLPQTFQILDTQDQLSAVKRLCKQFNVDEERFKPKELAYF
                     FAGCKEEGLRPRDVPVRDEDTRKKVELYQLYEEQCQREGVVDFGELMLRSYELLRDND
                     PVRVHYQRRFAHMLVDEFQDTNKLQYAWLKLLAGDMVDGRLQTRGSVIAVGDDDQSIY
                     AFRGARVGNMQDFVREFGVERQIKLEQNYRSYSNILDSANALIAHNSKRLGKNLRTSQ
                     GPGEPVRVYEANSDLAEAQWMVDEIRQLVQGGTATRSEIAVLYRSNAQSRVIESALFN
                     AAVPYRVYGGLRFFERAEIKHALAYLRLLENPHDDTSFMRVVNFPARGIGARSLESLQ
                     DVARQIGCSLHDAVSSISGKAGANLGAFVAMIDVLREQTQGQNLRAIIGQMLQASGLI
                     EHYRNEKEGADRLENLDELISAAESFVTQEGFGKDAVALPIDETGAPLTQSPASQGLD
                     PNAPLLNQPLVDADTGETLSPLAAFLTHAALEAGDNQAQAGQDAVQLMTVHASKGLEF
                     DCVFIGGLEEELFPHQNAMSDQGGLEEERRLMYVAITRARARLYLSHAQTRLLHGQTR
                     YNLRSRFFDELPEECLKWLTPRQQGFASYAPDMRAVGAYQSGARGQSGFKSEAFTSPP
                     VPPRKEAPAHGLRVGLAVFHTKFGEGKVLALEGAGVDARAQVNFPRHGTKWLALAVAK
                     LTIVE"
     gene            21892..23949
                     /locus_tag="PK518_02540"
     CDS             21892..23949
                     /locus_tag="PK518_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HRM47816.1"
                     /translation="MSKSVSDKVVRNHADNVPFSAILQARLSRRTVARGGLSAALALL
                     TGTGLAACGGGDGQGNAPAPVPAPGLKLGFESLPASMTDACVVPAGYVAHVLGPWGTP
                     LNDNAAEWDKDGNNSSTDLLHSTGMHHDGMHYFALEGSSTEGILVVNHEYIDEQALHP
                     AGPTMVGGKRPAEEVRKEINAHGVAVMHIRKSGGSWAIVKNSRYNRRFTSATPMALAG
                     PVSGTDWVKTPFSPTGRQVRGTNNNCGNGTTPWGTYLTAEENWAACFVNTDKANQPAH
                     QKRVGVSTSKGRYQWETAGGHGSEEQGEFARFNVTPSGADALSDWRNEVNGFGYLVEI
                     DPYDPGSIATKRTAMGRFAHEGAAYGLPQAGKPLAFYTGDDSRFEYIYRYVSDAAWDA
                     ADARRTDRLAVGAKYLDKGTLYVARFNDDGTGSWLPLTPDSVGKDGTRLGSRYATLDE
                     ILINTRGAADFMGATPMDRPEWTAVHPTNGDVYLTLTNNSSRNAGTGTNAANPRLNNV
                     NGHIIRWHDDAGANSFRWEIFVFGSDAAAAADTNRSGLSALNQLASPDGIAFDARGIL
                     WIQTDNGIDGGRNNAVARATNDQMLAVIPGALAEHKGPGPAINAANQGDLRRFFVGPN
                     AAEITGFACTPDFKTIFLNVQHPGNWPAYGSSDATLAPTGTVRPRAATVVIQKADGGA
                     IGV"
     gene            24277..25764
                     /locus_tag="PK518_02545"
     CDS             24277..25764
                     /locus_tag="PK518_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM47817.1"
                     /translation="MASLPALAACGGQVRLTQPVEFSAQELAGFRAMAPLRVLPLDAP
                     PMARHDTQDGYSGIGVDVWCFITERLGLRYEIIQRRDLSIAEKIRLVQEGQADVLLPL
                     SANSERAARGIFTRPFYESHYAVIARKDWRPEIRGLADLAPYRVGVLKGVASESLLKT
                     VMASDRLVVIDDVTSEALFQALRDGRIDVAVFSKAVFEEKRYQHEYFDLAVVHTLHDA
                     PRVYGFYFHASPRNQQIVQAFDRYLAVLDVSGAVAEHEDGERQFIQRYVIQRSWHLVV
                     LTAGAGALVLVVVLAALLYRYRRLARLLAASNEHILQQQQALQAVNQDLERQSQTDGL
                     TGLANRRQFDHALLREHARQQRTGAPLSLLMVDLDHFKHVNDRYGHAIGDDYLRAVAR
                     VLNTSVTRATDLAARYGGEEFVCLLPETAATDAQMLAERIRQGVSSMALPNELASPPH
                     LTLSIGVATLLGGKTDAAQLLAQADEQLYAAKHAGRDRVHAVVLR"
     gene            complement(25907..26734)
                     /locus_tag="PK518_02550"
     CDS             complement(25907..26734)
                     /locus_tag="PK518_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HRM47818.1"
                     /translation="MKLNIVPARTGLQWVRQGITVFWRQPMALSALFFMTMAAMSLAT
                     LLPLVGPAVALALLPSATLAMMVAAAEASQNRTPTPALMLVAFRTGKQRLNAMMSLGA
                     MYAVGFLGVMGLSALIDGGEFARVYLGGAPLTREVAESGDFQAAMWLAMLLYVPLSLL
                     FWHAPGLVHWHNVPPVKALFFSIVACWRNFGAFTVYGLSWLGVFLAAGLAMSLLVTLL
                     AAVGIGASMAGGIMVATAMMLAAMFFTSVVFTFRDSFAPPDTQTNGDEPAPGADPSH"
     gene            complement(26784..27734)
                     /locus_tag="PK518_02555"
     CDS             complement(26784..27734)
                     /locus_tag="PK518_02555"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRM47819.1"
                     /translation="MAVYTEVSNKEARELLRRLQLGKLLTLRGIEGGIENTNYFVTSE
                     AGEYVLTLFERLSAEQLPFYLHLMHHLAHHGIPVPDPQADKYGEILHQVAGKPAALAT
                     KLRGKSQLAPQAVHCTAVGNMLARMHLAARDYERQQPNLRALPWWNETVPVVLPHIGP
                     EQAELLTSELAYQNHVAAGSAYAALPRGPIHADLFRDNVMFEGEELTGCFDFYFAGVD
                     TWLFDLAVCLNDWCIDLPTGRHDEQRAAALLAAYQAVRPLTAAERELLPAMLRAGALR
                     FWLSRLWDFYLPREASMLKPHDPTHFERVLRERVRHPVHP"
     gene            complement(27809..28174)
                     /locus_tag="PK518_02560"
     CDS             complement(27809..28174)
                     /locus_tag="PK518_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47820.1"
                     /translation="MRAVYPFLLLAVASAPALAQNTSQNPSQPNTTERGQLSTQEQKA
                     DDRREPRVERIVVEDAGSRVDELRVGGQTQHITVQPKTGTPLPAYEVRANDGARARPG
                     NFNQSDSVTAPRVWNLRQF"
     gene            complement(28207..28686)
                     /locus_tag="PK518_02565"
     CDS             complement(28207..28686)
                     /locus_tag="PK518_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47821.1"
                     /translation="MGLDGGAMGRRAEPFTHSPAMKYIVALLACAASTLALADSEFKN
                     VPPLLHKSIQHGGVTAAQFDGGVLRAQLNKAEVTELVYQTFVFHNICAREWYEPQQFA
                     SLGLARVELLNAKGDQGFAFDTRGNVCTEMGRLGKNFRTFISQYTVACTASGCPPQR"
     gene            28730..>30422
                     /locus_tag="PK518_02570"
     CDS             28730..>30422
                     /locus_tag="PK518_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRM47822.1"
                     /translation="MSESKTLVLVDGSSYLYRAFHAMPDLRAVPGDPASSPTGAIRGM
                     INMLQALKKEVPADYAVCVFDASGPTFRDDIYAEYKANRSPMPDDLRAQIAPIHEVVR
                     LLGWPVLAIAGVEADDVIGTLAKTAAAQGLRVVISSGDKDLSQLVDERITIIDTMNGK
                     RRDVAGVTAEFGVPPALMVDFQTLVGDAVDNVPGVPKVGPKTAAKWLMEYGSLDALVA
                     SAGAIKGVAGENLRGALDWLPTARSLVTIKTDCELADHVAGLPALDSIAVGAEQTGEL
                     ADFYQKYGFKSLARALEPQAQPASGDLFAQPDASTVAVQAQRRDVAYDIILTWAQLDA
                     WLARIQAAPLVAIDTETTSLDELRAAIVGISFSVEPGAAAYIPLAHEGPDAPAQLPLA
                     EVLARLKPWLEDPARLKLGQHIKYDRHVFANHGVEVRGYAHDTMLQSYVLEVHKPHGL
                     ASLAERHTGRKGINYEDLCGKGAKQIPFAQVAVDKAAAYSCEDSDQTLDVHRVLWPQL
                     AAEEKLRAIYELEIACSETLFRIERNGVLIDAQQLASQSHALGQRILQLEKEAYDI"
BASE COUNT         5096 a        10179 c        10367 g         4780 t
ORIGIN      
        1 ctgctgcgcg gcatcgtcgc gatcgcgcgc gagcatgagc tggtgctgtt tgccgacgag
       61 gtctacgaca aggtgctgta cgacggcgtg ggccacacgg ccatggccag cctgtcctcc
      121 gacgtgctca cgctgacctt caattcgctc tccaaggcct accgctcttg cggctaccgt
      181 gccggctgga tggtggtctc gggcgacaag gctgcggcgc gcgactacat cgagggcctg
      241 aacatgctgg ccaacatcaa gctcggctcc aacgtgccgg gtcagtgggc cattcagacg
      301 gcgctgggcg gctaccagag catcaacgac ctggtgaaag agggcgggcg cctgcgccgc
      361 cagcgcgacc tggcctatga gctgatcacg cagattccgg gcgtcacctg cgtcaagccg
      421 caggcggcgc tgtacatgtt cccgcgcctg gatccagaca tgtacccgat cgccgacgac
      481 cgccagttct tcatggaggt gctgcgcgcc acgcgcgtga tgctggtgca gggctcgggc
      541 ttcaactacc cggacaacca gcatttccgc atcgtctttt tgccgcacga ggatgacctg
      601 cgtgaggcgg tggcgcgtct ggcggccttc ctggcaacct atcgacagcg caacgccaag
      661 gcggcttgac tattttttta tagctagtgg cgcttgatag tagggcgcta gagccatttt
      721 ttactggtaa aactgaaatg aaacccatac aagtaggcct gctgggcatc ggcaccgtgg
      781 gcagcggcgt gttcaacgtc ttgcaacgca accaggaaga gattcgccgc cgggccggcc
      841 gcggcataga gatcgcctgt gtggccgatc tggacaccgc gcgcgcccgc tccatcgtcg
      901 gcgagggcac gcgcgtggtg ggcgatgcgc gcgaggtcat tgccaacccc gagatcgaca
      961 tcgtcatcga gctgatcggc ggctacggca tagccaaaca actggtgctg gaggcgattg
     1021 ccgccggcaa gcatgtggtc acggccaaca aggccttgct ggccgtgcat ggcacggaga
     1081 ttttcaaggc cgccgccgac aagggcgtgg tcgtcgccta cgaggcggcc gtggccggcg
     1141 gcatccccat catcaaggcg ctgcgcgagg gcctgtcggc caaccagatc cagtgggtgg
     1201 ccggcatcat caatggcacg accaacttca tcctgtccga gatgcgcgac aagggcctgg
     1261 acttcgacgt ggtcttgaag gaggcgcagc gcctgggcta cgccgaggcg gacccgacgt
     1321 ttgacatcga gggcgtggac gccgcgcaca aggccaccat catgtcggcg attgccttcg
     1381 gcattccggt gcagttcgac aaggcctatg tggagggcat caccaagctg agcgccgccg
     1441 acatccgcta cgccgagcag ctcggttacc gcatcaagct cctgggcatc accaagcgca
     1501 ccgacaaggg cgtggagctg cgcgtgcacc ccagcctggt gccggccaag cgcctgatcg
     1561 ccaacgtcga gggcgcgatg aacgccgtgg tggtgcacgg cgacgccgtg ggcaccacgc
     1621 tgtactacgg caagggcgcg ggctcggagc ccacggccag cgccgtgatc gccgacctgg
     1681 tggacatcac ccgcctgatc gaggccgacc ccgcgcaccg cgtgccgccg ctggccttcc
     1741 agaaccacac gctgggcgcg gcggggcagg acctgcccgt gctgcccatg gccgaggtgg
     1801 tcaccagcta ctacctgcgc atccgcgtgg ccgaccaggc aggcgtgctg gcccggatca
     1861 ccggtctgct ggccgacgcc ggcatcagca tcgacgccgt gctgcagcgc gaggccgacg
     1921 aggtgggcgg cgagggctcg acgcaaaccg acgtcatcat cctcacccac gacacgcgcg
     1981 agggcgacat ggacggcgcc ctggcccaga tccaggcctt gcccacggtg ctggcaccga
     2041 tcacgcgcat ccgcaaggaa gagctgaact gatggcttaa tctggccaga atgcgtaaaa
     2101 ttctggctag attgacgcca tcaggaggtc gccatgtccg cagtctggca agtgcaagaa
     2161 gccaagaacc acttcagcga agtgatcgat cgcgccctta ccgaagggcc gcagaccgtc
     2221 acgcgccatg gcaagcccgt ggtgcgggtg gtcgcggtga ccgagggcga catgcaggag
     2281 cctgccgatg acgggttcct ggagtttttg ctcagcgcgc ccaaggtcga gggcgggcta
     2341 cctgagatgc cgcgcgacac aaacccgggg cgctcgccca tgtttggcga ggattgactc
     2401 gccatgcgct ggctgctcga tacctgcacg atatcggagt cgacttatcc ccaaccccat
     2461 gctggcgtgc tcgactggct gcgtcggcac caccatgatt gcgccatcgc tgcggcatcc
     2521 tttaccgaaa tccattacgg catcgcctgc ctgccacatg gcgccaagcg caatcgccta
     2581 caggcatggg cgctggagct ggctcagcag ttcgatggtc gtattcttgc ctccgacgat
     2641 gccgtgtggc gccagtttgg cgacctcaag gcttcgttgc gcgccatggg ccgtatgcag
     2701 gatgccctgg acatggtgat tgccgcgacg gcgctgcacc acggcctgcc cctcgtcacc
     2761 cgcaacacgc gctactttga agacacgggc ctcaggctcg tcaacccctg ggccgacgtg
     2821 tcccaggctt gagtaacacc atgcaatacc tctccacccg cggccacccc gaacgcaagc
     2881 gtttttgcga catcctgctc gaaggcctgg cgcccgacgg cggcctgtac ctgcccgaga
     2941 cctacccgca gatcgacgac gctgcgctga cccagctgcg ccgcgcctac aacgaggaag
     3001 gctacgccgc gctggcgttt cgcatcctct cgctgtacat cgacgacatt cccgccgccg
     3061 acctgaaggc cttgtgtgcc aagacctata cgcaagaggt cttcggcacg aaagagatcg
     3121 tgcccctgcg ccagctggaa gacggcctgt ggatcgaagc gctgtccaac ggccccacgc
     3181 tggccttcaa ggacatggcc atgcagctgc tgggcaacct gttcgagtac gagctggcgc
     3241 gccgtggcga agagctgaac atcctgggcg ccaccagcgg cgacaccggc agcgcggccg
     3301 aatacgccat gcgcggcaag cagggcgtgc gcgtctttat gaccagcccg cacggccgca
     3361 tgagcccctt ccagcaggcg cagatgttca gcctgcaaga cgagaacatc cacaacctgg
     3421 cgatagaggg cgtgttcgac gactgccagg acatcgtcaa ggccgtgagc aatgaccatg
     3481 ccttcaaggc ccgctacaag atcggcaccg tcaactccat caactgggcg cgcctgctgg
     3541 cccaggtggt gtactacttt gccggctaca tacaggccac gcaaggcaac gagcagaagg
     3601 tcagcttcac cgtgcccagc ggcaacttcg gcaacatctg cgccggccat gtggcgcgcc
     3661 agatgggtct gccgatcgac cgcctggtgg tggccacgaa cgagaacgac gtgctcgacg
     3721 agttcttccg caccggcgtc taccaggttc gcgccagcga gcacacctac gagacgtcca
     3781 gcccctcgat ggacatcagc aaggccagca acttcgagcg cttcgtgttc gacctggtgg
     3841 gccgcgacgg cgcgcgtacc caggccctgt ttgccgacgg cgtgggccgg gcggggcgct
     3901 tcgacctgag ccaggacgcc gtgttccagg acacggccgg caaatatggt ttcgtgagcg
     3961 gcaagagcac gcacgccgac cgcctggcca ccatccgtga cacgtatcag cgcttcggcc
     4021 agatcatcga cacccacacc gccgatggcg tgaaggtggc gcgcgagcgg cgcggcgacc
     4081 tggccgtgcc gatgatcgtg ctggagacgg ccctgcccat caagttcgct gagaccatcc
     4141 aagaggccct gggccgctcg cccgagcgtc cggccaggtt cgagggcata gagctgctac
     4201 ccaagcgcgt gcagcagctg ccggccgatg tcgagcgggt gaaggcctat atcgccgcgc
     4261 actgtgattg aagtcaaatg agggcgtagc gcttgctggt aggctgctga cagctatcat
     4321 ttttgcataa ggagcggcgg tatgaaggtg ctgggctttg ctggttattc gggcagtggc
     4381 aagaccacgc tgctggagca gctgattgcc ctgctgcgcc tgcgcggcct gcgggtgtcg
     4441 gtggtcaagc acgcgcacca caggtttgat atcgaccacc agggcaagga cagctggcgc
     4501 caccgcgagg ccggcgccta cgaggtggtc gtggcgtccg accggcgcat ggcgctgatg
     4561 cgcgagtacg agcagcccca tgcgatcgat ccgcatgagc tgctggccga actcgacccg
     4621 cgcgtcgatt gggtgctggt ggaaggcttc aagcacagcg acctgcccaa ggtcgaggtc
     4681 tggcgacccg ccgcgcccgg ccagccggag cgccccgtgc actacggtgg cgatgaccat
     4741 gtggtggccg tcgccaccga cgcggcacag ctgccccggc ccaccgcgct gcccgtgctg
     4801 aatctgcgcg accccgaggc ggtggcgcag tggatgctgg agcagggcgc gcgttttgac
     4861 tatctgcccc aagccgtgga ggcctgcgaa tgacagcgcg cacacccctc ataccccttg
     4921 acgacgccct gaccgagctg ctggcccagg ccaggccgct ggccggcgtc gacaccgtca
     4981 gcaccttcga tgccgacggc cgtgtgctgg cgcaggcggc catctcgcct ctgcaggtgc
     5041 cgcaacagga caacagcgcc atggacggct atgcgctgcg ctgcgccgac gtcgctgcgg
     5101 cgggcgtgga gttgcgcgtg tcgcagcgca ttgcggccgg cgacagcggc cagcccctgc
     5161 tgcccggcac ggccgcgcgc atcttcaccg gtgcgcccgt gccagccggg gccgacgccg
     5221 tggtgatgca ggaggactgc gaggcgctcg acgacggcca gcgcgtgcgt atcaaccacc
     5281 tgcccagccc gggccaggcc atacgccgcg ccggcgagga catcaccctg ggcgcccagg
     5341 tgctggccgc cggcacgcgc ctgaccccgg ccgagctggg cctggccgcc agcattgggc
     5401 tggcgcagct gcaggtggcg cgccgcccgc gcgtggcctt gttctccacc ggcgacgagc
     5461 tcgtcatgcc cggcgaggtg gcgcccgcgc aaatgcgccc cggtgccatc tacaacagca
     5521 accgcttctt tctgcgcgcc atgctgcagc gcctgggctg cgcggtgact gacatggggg
     5581 tggtgccgga tcggctggac gccaccgtgg ccgcgctgcg cgaggccgcc caggggcacg
     5641 atctgatcct gaccagtggc ggtgtctcgg tgggcgagga agaccatgtc aagcccgccg
     5701 tgcaaagtct gggcagcctg gacttgtggc agatcgccat caagcccggc aagccctttg
     5761 cttacggccg catcggtggc gcgcatttca tcggcctgcc cggcaacccg gtatcgagct
     5821 tcatcacctt tgcgctgctg gtgcggccct tcctgctgcg gctgcagggg gtggtgaatg
     5881 ttgctccaac aacaatagct gccagcgctg gatttgattg gccaagagtc gataaacgcc
     5941 gagaattctt gcgtgcccgc catggtgcgg acggccgcct ggagctgttt gccaaccagg
     6001 gctcgggcgt gctcacttcg gtcgcctggg gtgatggcct ggtggacaac cccgcaggcc
     6061 acgcaatagc gcgcggcgac caggtgcgct ttctgccctt tgccgagctg ctgtcatgac
     6121 cgcgccgctc accatcaccg tgcgctactt cgcctccatc cgtgaggccg tgggccaggg
     6181 tcaggaaacc ctgcgcaccg ctgcccccac cctgggcgcg ctgcgcgacg agctgatagc
     6241 gcgcggccag ccctgggccg attgcctggc gcgcggccga gccgtgcgca tggcgctgga
     6301 tcaaaccatg gcccaggagg ccacgccgct ggccgacggt gccgaggcgg cgttctttcc
     6361 gcccgtcacc ggcggttgac cgtcaatcgc ctgtcgttgg cagggccggg ctgcgccagc
     6421 ccaacagctc ggccagccgc agcgtgatcc agcgcgcctc cggtgcgctc acgccggcat
     6481 cggggctggc caggcccgca tgcatgccca gcagcacgcc ttcctcggag cgcgcgccgc
     6541 cgtggtagag ctgctgcgcc tgctctacca ggtgctgggc caggtcttcg cacaactcgt
     6601 agcgtgcccg cagctcatcc acgggcagca acaggcgctg acgcgcgtcg ctgtgcaggg
     6661 cgatgaagga gggcgggatc aggatctggt tgtcgtcatg catgggccgt attgtcgaca
     6721 aaggcacgat caccgaccct gagaggccgc cgcaatcagg gctgcttctg gtctgctggc
     6781 ggcgcgtcgt tgcgcgtgaa cagccagaca aagaacacca gcatgcacag catgaagcct
     6841 atgcccacgg cgctgaacag gccgacctcg gtggtgaaga gttgcttgag cagttgcatg
     6901 gcaggttccc ttgtagttga gtatgtgctt atattgcaac gcaacacgaa gggcgcgtgt
     6961 ttgctttagg tcaagaaata ccgcgcagtc gttggtggct ttgtcagcct tgatatggct
     7021 caagcctgac gggcgtcagt cgggcacgaa caggcgttca aagcgctgtt gatcgcttgc
     7081 cagctcaaag gtcaccagct gccccatctt gttgtcggcg cgccggtggg cggcaaacag
     7141 gctgtggcgg cccgccagct cgaagctggg cagatcgatc agcgcatagg ggtggggcac
     7201 gaaggtctgg aactcgaaca ccacgcggtg ctggttgcgt ggcgaggggt agagcgtgta
     7261 gtcgtcggtg ttgatggtct gcagggccat ggagcaggag cggggcgtta aagtgccaat
     7321 tttgcgcctt gatccaatga cccgcgtttc catccagacc caggatttcg atgtctcggc
     7381 cgagctggcc cggctgcgcg cccaggacgc gcgcgtgggc gccgtctgct gcttcgtcgg
     7441 cacggtgcgt gaccgcaacg atggtgatgc cgtggcgacg atggagctgg agcattaccc
     7501 cggcatgacc gagaagtcca tcgaggccat gatagctgcg gcgctggcgc gtttcgacct
     7561 gtacggcgtt cgcgtgatcc accgcgtggg gctgttggcg ccgctcgatc agatcgtgct
     7621 ggtggccgtc accagcgcgc accgcggcga gagtttccag gcctgcgaat tcctcatgga
     7681 ttacctgaaa acccaggcac cgttctggaa gaaggaagaa acgccccagg gcgcgcgctg
     7741 ggtggacgcg cgcgtggccg acgacgcggc gctggcgcgc tggggcattg ccgcgccagc
     7801 gcaatttgac tgactttgat ggcgcggtgg cttgaaatcg gggcgttcca accctagtta
     7861 gcagcaagtt ggaggtaaac catgcgtttc gacaaattca cgaccaagtt ccaggaagcc
     7921 ctgtccgacg cccagtccat cgcgctgggc catgacaacg cctatatcga accggtgcat
     7981 atgctggtcg ccatgctgcg ccaggatgag ggtccgcgcg cgctgctcga gcgcgccggc
     8041 gccaacgcgc ccggcctgct gaaggcggcc gaggccgccg tgaagcgcct gccccaggtg
     8101 caaggccagg atcaggtgca gggcagcccc gagctgggcc gcgtgctgca ggccaccgag
     8161 aaggaggcga tacagcgcgg cgaccagttc atcgccagcg agctgttcct gctggccctg
     8221 atcgacagca agggcgaggc cgcgcagatc gccaaggaca acggcctgtc ccgcaagagc
     8281 ctggaggcgg cgatagacgc cgtgcgcggc ggccagaagg tggacagcgc cgaggccgag
     8341 ggccagcgcg aggcgctgaa gaaatactgc ctggatctga ccgagcgcgc tcgcctgggc
     8401 aagctcgacc ccgtgatcgg ccgcgacgac gagatccgcc gcgccatcca ggtgctgcag
     8461 cggcgcagca agaacaaccc ggtgctgatc ggcgagcccg gcgtgggcaa gaccgccatc
     8521 gtcgagggcc tggcccagcg catcgtcgag ggcgaggtgc ccgagtcgct gaagaacaag
     8581 cgcgtgctgg tgctggacat ggcggggctg ctcgccggcg ccaagtaccg cggcgagttc
     8641 gaggagcggc tgaaatcagt gctgaaggag gtggcgcacg aggaaggccg catcatcctc
     8701 ttcatcgacg agatccacac catggtcggc gccggcaagg ctgaaggagc gatagacgcc
     8761 ggcaacatgc tgaaaccggc cctggcgcgc ggcgagctgc actgcatagg cgcgaccacg
     8821 ctggatgagt accgcaagta catcgagaag gacgcggccc tggagcggcg ctttcagaag
     8881 gtgctggtgc aggagcccag cgtggaggac accatcgcca tcctgcgcgg cctgcaggtg
     8941 cgctatgagg cgcaccacgg cgtggacatc accgacccgg ccatcgtcgc cgcggccgag
     9001 ctctcgcacc gctacatcac cgaccgcttc ctgcccgaca aggcgattga cctgatcgac
     9061 gaggccgcgg ccaagatcaa gatcgagatc gactccaagc ccgaggctct ggacaggctg
     9121 gagcgccgca tgatccagct caagatcgaa cgcgaggcca tgaagaagga gcatgacgag
     9181 gcttcgcaaa agcgcctggc cttgatcgag gaagaactgg ccaacgtgga gcgcgaatac
     9241 gccgacctgg aagagatctg gaagatggaa aaggccagcg cccagggcag cgagcagatc
     9301 aggaaggaca tagacgccat ccgcacgcag atggatgacc tgaagcgcaa gggcgacttc
     9361 aacaaggtcg ccgagctgca atacggccgc ctgcccgaac tggaaaagcg cctgaaggag
     9421 gcgcaggaca gcgaggccga tggcgagacc ccgaagcacc gcctgctgcg cacccatgtg
     9481 ggcgccgagg agatcgccga ggtggtcagc cgtgccaccg gcattcctgt ggccaagatg
     9541 atgcagggcg agaaggacaa gctcctgcac atggaggaca agctgcacga gcgcgtggtc
     9601 ggccaggacg aggccatatc agccgtggcc aacgccatcc ggcgctcgcg ctcggggctg
     9661 tcggacccga accggccgct gggcagcttc ttgttcctgg gccccacggg cgtgggcaag
     9721 accgagctgt gcaaggcgct ggcgggcttt ctgttcgaca gcgaggaaca cctggtgcgc
     9781 atcgacatga gcgagttcat ggaaaagcat agcgttgcgc gcttgattgg cgcgcctccc
     9841 ggctatgttg gctatgaaga gggcggctac ctgacggagg ccgtgcggcg caagccctac
     9901 agcgtgctgc tgctcgacga ggtggagaag gcccaccccg atgtgttcaa cgtgctcctg
     9961 caggtgctgg acgacgggcg cctgaccgac ggccagggcc gcaccgtgga cttcaagaac
    10021 acggtcatcg tcatgaccag caatatcggc tcgccgctga tccaggccat ggtgggccag
    10081 gacgcggacg acatcaagga agcggtgtgg ggggaactaa aaaaccattt ccgccccgaa
    10141 ttcctgaacc gcatcgacga gacggtggtg ttccacgggc tggatgccag gcacatcgag
    10201 gccattgcgc gcatacagct gcatttgctg caagcgcggc tggccaagat ggatctgcag
    10261 ttggaggtgt cgcccgcagc gatgggcgag ctggccaagg tggggttcga cccggtgttt
    10321 ggcgcgcggc cactcaagcg tgcgatacag caacgcatcg agaatccgct gtccaagctg
    10381 ctgctgcagg ggcactatcc gcccaagagc gtgatacccg tggatgtcga tccggtgcag
    10441 gagccagggg tatttcactt tggccaggcc cgccagctgg cttgaagcct accatggagc
    10501 gcgcggtgcg ggcctggccc gcgccggcac tcctgtctga aagggggatg gtttgaatga
    10561 tgtagtgacc ggtgtgatcc ggtgggtttt gcgcctggtg gtgttggcca tgggcgtgct
    10621 cttcttcctg agcctgctgg ccgccgcggc cgtgctggcg ctggtctggg gcctgcgtgc
    10681 gctgtgggcg cggctgacgg gcaggccggt cacgccgtgg gtcatgcgca tggatccgcg
    10741 caccggctgg agcaccgtgt accgcagcac ggcgcgctgg acggcccagc gcccggagcc
    10801 gccgcgccgg gacgatcacg acgtcaccga cgtggtgccg cgcgaaatcc gctgaaaacc
    10861 cctcacattt gcctgacgcg tggcctgccc ggcctgcgcg gccatccacc atgcgcgcca
    10921 ctttgccgga cgacattcgc cacgtttttg aattgcagcg ccaggccagc cgcgcccagc
    10981 ccgatacccc cctgacgctg cgccgcgagc gcctgctgcg cctgtccaag ctgatcgatg
    11041 agcatggccc ggccctggcg gccgcagtcc aggccgactt cggcatgcgc tcgccgcgcc
    11101 tgaccgaggt ggcggatctg ttcatgctgc gtgcccagct tgcgcacacc ctgcgccacc
    11161 tggcgcgctg gtgccggcgc acccgggtgc gcaccccgtt gtttctgctg cccgcgcgcg
    11221 gctggatcga gcgccagccg ctgggcgtgg tgggtgtgat ctccccctgg aactacccgg
    11281 tgcagctggc cctggcgccg gtcataggcg cgctggcagc gggcaaccgc gtcatgctca
    11341 agcccagcga actcaccccg cacacggcgg cgcagctggc aagcctggtg gcgcagttct
    11401 ttgcgcccga cgagttctgc gtggtccagg gcgacgcggc caccgcggcg ctggtggcct
    11461 cgctgccgtt tgatcacctg gtgtttaccg gctccaccgc cgtggggcgc aaggtggcgc
    11521 aggccgcggc cgccaacctg acgccgacca cgctggagct gggcggcaag tcgccggcca
    11581 tcatcgatac cgattgcgac ctgcgggagg cggcgctgaa gatcgtccac ggcaagctgc
    11641 tcaacgccgg ccagacctgc atcgcccccg actatgtgat gctgccgcgc ggcagcgagg
    11701 ccgcctttgc cgaggcctac cgcgccgccg tggcgcggct gtttccgtac ttcgagggca
    11761 acccggacta cgccgccatc gtcagcccgc gccacctggc gcggctgcgc accatgttgc
    11821 agcaggccca gaccctgggc gccgaactgc aggtgctgca aagcgtgcca gggtcgccgc
    11881 caccaccgcc ccagggcata ggcgacggcg tgggccggca gatggcgccg gtgctggtgt
    11941 ttggcgccac gccggccatg cagctgatgc aggaggaaat cttcggcccg gtgctgcccg
    12001 tgctgagcta cgagcggctc gatgacgcca ttgcccacat caacgccggg ccgcgcccgc
    12061 tggccctgta ctggtttggc cgcggcgagg ccgcgcgtga tgacgtgctg cgccgcacgg
    12121 tcagcggcgg cgtgtgcgtc aacgacacgc tgctgcatgt ggcgcatgac aacctgccct
    12181 ttggtggcgt gggcgacagc ggctggggcg cgtatcacgg tgaggccggc tttctgcgct
    12241 ttagccacca gaagtcggtg ctggcgcagt cgcgctgggc ccagggccac ctgctgtacc
    12301 cgccctatgg tgcgcgtttt gaccgggtga tggcgctgct gcggcgcctg gtgtagcgcg
    12361 ttggcgggtt gccgcacgcc tgcctgccac agatggaggc gtttgcgtac aaaaacgggg
    12421 ctgggcacaa ggaaacttgc cgtgcttgga gacaaaaaac catttgaggg aaaataccgg
    12481 gttttcccaa attcaccggt tctcgttttc ccatgtccag tatccaggtt cgtcccgcca
    12541 cacttcgcga tgccaaggcc attgcccagg ttcacaccac cgccgccttg gaagcctacc
    12601 gcggtctggt gcccgacgat caattgaaat ccatgtcttc ggtcgagaag cgccaggcct
    12661 actggcgcga ggccatcgag tattgcgagc cccaggtgca ggtggccgtg gacggcgaca
    12721 agatcgtcgg ctttgtcggc ttcgaccgct cgcgcgatga aaagacgcgc aacaccacgg
    12781 gcgaaatctg ggccatctac gccgctccca cgcattggga caagggcgtg ggcgtggccc
    12841 tgtgggacgc cgcccgcgat ggcctgcagg aagagggctg caccaccgtc accgcctggg
    12901 tgccgctgcg caacgagcgc gccctgcgct ttcacgagat ggctggcttc aagcgcgaga
    12961 tgaccaccgc caagactgcc ctggtcggcg gcgtgaagat cgaggaagtg cgcctcaagc
    13021 gccccgtcaa ctgaagccac tgttgcaggg ccggcgatga tcgaatggag cgaacagggc
    13081 gaggcgcagc gcgcccgctg gcactccgag agtggcgccg ccgcgcccgc ccgcgtgcag
    13141 ccggccgacg acacgctgag cgccgacagc gcctaccgcc tggcctgcga gggcacggcc
    13201 ctgctgtggc gcggcgactt ccagaatgcg cgccagctgc tgcaggcctt gatgcgccgc
    13261 gccgaccgca agaaccccaa ggcagccgcc aaggccgcgc agaagctggc cggggccacc
    13321 ccgtccgagc gctttcacct gcaccgccag gcgcaggcgc agcgcgcgcg cacgctgggc
    13381 gcgctgctga taccggtagc tggcgactac tccatccccc tgcgccgcgc gcccgactgg
    13441 cgcgaggcct gcaccgaggc ctggggcccg gccgatggca cgcccagtgt ggtcgcgctg
    13501 cgtgagttgc tgggcatggt cggcgcgcat gaatggcgca agaagggcgt ggagatcccc
    13561 gccctgggcg ccgcgccggg caaccgcatc cacccgcact acggcgtgtt ctcgccgctg
    13621 cgcggcgaat acatcgacct ggtggcccag gcgctgctgc cgtccaccga gctggccttc
    13681 gacatcggca ccggcaccgg cgtgctggcc gccgtgctgg cgcgccgggg cgtgcgccgc
    13741 atcgtcgcca ccgagctggc gccgcgcgcc ctggtctgcg cccaggacaa cctgcagcgc
    13801 ctgggcctgc agggccgggt ggaactgctg gccgccgacc tgttcccgcc cgctcgcgcg
    13861 cccctcgtgg tctgcaatcc cccctggcta ccggcgcggc ccagctcggc cgtggagcag
    13921 gcggtgtacg acgagggcag ccgcatgctc aagggctttc tggccggcct ggccgcgcac
    13981 ctgtcgcccg gcggcgaggg ctggctgatc ctgtccgacc tggcggaaca cctgggtctg
    14041 cgctcgcgcg atgagctgct gggctggatc gccgcagccg gactgcgcgt tctcggccgc
    14101 caggacacac gcccgcgcca cagcaaggcc caggacgccg gcgacatgct gcacgccgcg
    14161 cgcgcggccg aggtcacgtc gctgtggcgc ctggccgttg cgcaatgatc gctatataaa
    14221 taatagctgc ttgcgcttat tggtaaagcg ctagaggtcg ttttgactca aacattcccg
    14281 cgcaagcccc tgctgctggc tcccatggaa gggctgctgg acttcgtgct gcgcgacgtg
    14341 ctgactcgcg tcggcggcgt cgatcgctgc gtgtccgagt tcatccgcgt ctcgggcaat
    14401 ctgctgaccg acagggtgtt tctgagctac atccccgagc tgaagaacgg cggccgcacc
    14461 ctggccggcg tgcccgtgcg cgcgcagctg ctgggttcgg acccgggctg catggccgag
    14521 aacgccgccc gcctggccgc cctgggcccc gagggcatag acctgaactt tggctgtccg
    14581 gccaagaccg tgaaccgcca cggcggcggc gcggccctgc tgcgcgagcc agaatcaata
    14641 gcggccgtgg tggccgccgt gcgccgcgcc gtgcccgctc cgctgcccgt gtccgccaag
    14701 atgcgcctgg gctatgacga cgccggcctg gcgcgcgaat gcgcccaggc catggagcag
    14761 ggcggcgcca cagagctggt ggtgcatgcg cgcaccaagg ccgatggcta tcgcccgccc
    14821 gcgtactggg agcagatccc cgccattcgt gcggcggtcg ccatccccgt ggtggccaat
    14881 ggtgagatct ggagcgtggc cgatgcgctg cgctgccgca gcctgtcggg ctgcgacgcg
    14941 ctgatgctgg gccgcggcat ggtggccgac cccggcctgg ccctggccat acgcgcgcac
    15001 gaccaggggc aggcccacca gcccctggcc tggcgggccc tgctgccgca gatcgggcgc
    15061 ttctgggatc tggtctgcgc cgacctggag ccgcgccagc gcgccggccg gctcaagcaa
    15121 tggctgaacc tgctgcgacg gtgctacccc gaggcgcagc gggcgttcga cgaagtgcgc
    15181 gtgatgaccg accagcgtgt gattacggcc tgggtgcggc aggtggccga tcaataagac
    15241 ccgatcagca aatcatgcgc cgcgcgcagg cgctgcacgt cgcgccccca gcggcgatcc
    15301 tgcggcaggt ggcgcagcca ggtcaggcgc gtcagcgtgc cctgcagcgc cgcctgaaag
    15361 tccgcgccac gcagcggctg ggtcaaccag tcgttccaga gggcgcgcgc ctcctgctgg
    15421 gcgccggctt gccgcagcgc cagtgccgtg gaatgcgcat aggccagcgc atcgcgcagc
    15481 tggcacaggg ccaggggcag ggcgcttaaa gggtcgtcct cgaagtctat gcaggcaatc
    15541 gcgccgtccg ggcagcgcac catgttgcgc gcaaaggcct ggctcaggca ttgctgatgg
    15601 ccatgcacgg cgtccagcgt ctgcaggccc aggcgccaca gcgccagcac ggcggtgggg
    15661 ccggcagcca cggcggcctc gatctcgtcg cccagcgacg gcgtgggccg gcccggtgtg
    15721 cccaggtcgc gcatcaaaaa ggcgtcatcg caggcggcca gcacctgcgg cacgcgcagg
    15781 cccagcgcgc ccagggtgcg caggcgtcgg agctcggtgg caatcgccgg gcgcccgccc
    15841 aggttgggca cggggcgcag caccggcagg cgcaggacgc cggcggccgc gcccagcagg
    15901 cggtagcgcc acataccatg gcgcggcccg gcgcgcttga gccagacctg ctcccccgcc
    15961 agcatgtagc gtgccaccag cggttgttgc tgcggccatt gctcctgcag gaagcggctg
    16021 tagccggtgt tgttcaaaat tgcgcccgtc ccgccacgcg ggcaatcagg cgcagatccg
    16081 ggccgatctg ctgtatgtcg tggaactcca tgcgcagccc ttgcgccagc gcctgcagcg
    16141 gcccccagct ggccaggcca tgcgcgccgc tgcccagcag caggggcgcc aggtagagca
    16201 gcatttcatc gaccaggccc tcgcgcacaa aggagccgtt gagtttgtgc ccggcctcca
    16261 ggtgcagctc gttcacgccg cgtggcccta agtcgcgcag cagggccggc agatccacct
    16321 tgccgtgggc attgggcagc tgaatgaccg tggcaccgcg cgcctcaagc gcctgttttc
    16381 gcgtgccgtc ctcgtgggcg gcgtagatga acacgcgcct gtcgggcacg ccgaacagcc
    16441 gtgccgtggg cggtgtctgc agccggctgt ccaccaccac cagatgcggc tggcgcggtg
    16501 tggccacttc gcgcacgttg agcagcgggt cgtcgtccag caccgtgcca atgcctgtca
    16561 gcacggcgca ggcgcgtgcg cgccaggcat ggccgtcggc gcgcgcggcc ggtgccgtga
    16621 tccactgact ggcgccgttg gccagcgccg tcacgccgtc cagcgaggcc gcggccttca
    16681 tgcgtaccca gggcgtgccg cgctgcatgc ggctgaaaaa gccgatgttc aactcgcgcg
    16741 cctgtgcggc gcccggcccc acctcgacct ccacccccgc ggcgcgcagc cgcgcaaagc
    16801 cctggcccgc cacctggggg ttggggtcgg caatgctggc caccacgcgc cctatgcccg
    16861 cctgtatcag cgcatcgcag cacggccccg tgcgcccctg gtgcgcgcag ggttccagcg
    16921 tcacataggc cgtggcgccg cgcacgtcat ggccgcgggc ggcggcatcg cgcagcgcca
    16981 ctacctcggc atgcgggccg ccggcctgct gggtaaaacc ctggccaagc acggcgccgt
    17041 cgccgccggc gatcacgcag cccacgcgcg gattcgggtt ggaaagacaa agcgcctggg
    17101 ctgcgagccc aagcgcctgc tggatgaagg gtgtggcgcc tgtcatgacg cagcgatggt
    17161 agcgcgcaga accatgcgtt tgtgcaaacc gcgcaggcct gtcaattcct gtcgggttcg
    17221 gaaataaaaa agccggaagt tgcatcacaa cttccggctt tctggaatag ctggtgggcc
    17281 ctgaaggatt cgaaccttcg accaacggat taagagtccg ctgctctacc aactgagcta
    17341 agagccctct cttttcagag gcgctgcaat gcagcacagc cgcgaattat gcaccaagtt
    17401 tgggtgcctc cagaaaagat ggcaaaaaat tcacatcagc aggtgctcgc ccgcgttgtc
    17461 gccgcccagg atcacgtagt tcaccttgcg gatgtccatg agttttttgc cgcccgagta
    17521 gctgatggag ctttggatgt cctgctccat ctcggtcagc gtgtcggcca gcttgccctt
    17581 gatcggttcg aggatgcgtt tgccctccac atgccggtac tcgcccttgt tgaagtcgct
    17641 ggccgagccg tagtactcct tgtacagctc cccgtccacc tccacggtat ggccggggga
    17701 ctcttcgtgg cccgcgaaca gcgagccaat catgaccatg gtggcgccaa agcggatgct
    17761 cttggcgatg tcgccgtggc ttctgatgcc gccgtcggca atgatgggct tggtggccac
    17821 gcgcgcgcac cacttcatgg ccgacagctg ccagccgccc gtgccaaagc cggttttcag
    17881 cttggtgatg cagaccttgc ccgggcccac gcccaccttg gtggcgtcgg cgccccagtt
    17941 ctccaggtcg atcacggcct cgggcgtggc cacgttgccg gcgatgacga aggactgcgg
    18001 caggtgtttt ttgaggtagc cgatcatgtc gcgcacgctg tcggagtggc cgtgggcgat
    18061 gtcgatggtg atgtactcgg gcgtcagccc ttcggccttg aggcgatcga cggtgtcgta
    18121 gtcggcctgc ttgatgccca gcgagatcga ggcaaagcag ccctgggcgc gcatgtcgcg
    18181 cacgaaccgc acgttgtcca gatcaaagcg gtgcatgacg tagaagtagc cgttctgggc
    18241 cagccaggcg cagatttttt cgtccaccac ggttttcatg ttggccggca ccaccggcag
    18301 cttgaaacgg cgcccgccca gttccacgct ggcgtcgcat tcggatcggc tttgcacgcg
    18361 gcacttgcgt ggcagcagca ggatgttgtc gtagtcgaag atttccatga ggttccaagc
    18421 tccttagaaa aggtcaccag ggcaggattg cactggcgtg gggcgcttgg cttgggctcc
    18481 ggctttgcgg gcactgccgc agctggcggc gccgaaccct gtttgcacag gcgcaaaaaa
    18541 accgggcgtc aagaaacttg ggcccggtga ttgattgtac gggtgcgcag gatgtgcaga
    18601 aggtcgcggg gtctattcgg ggcgcggcag ggggaccggc tggccggcgc acagcgcctg
    18661 cgctgcggcc acggtgcgcc cggcagcgtc ctgcacccag cccagcagcc ccaggcgctc
    18721 gacatcggcg ccgtcgggaa tgcgcatggg gcgcagatcc gacagcgcac cgccgggcca
    18781 ttcgccgtgc caggcattgc gcaccacatg gcgcgcgacg ggactggctt cggcgccggc
    18841 cggcagttgc tccaccagca ccagccagaa gcgcagcggc gggcgcgcct tgggtggcgg
    18901 gctgaagcgg atgatggtgc ccacgtagtc gttgacggcc ggccccatgg ccacgcgcag
    18961 gctgccgggc gcggtgatga gcgagtcggt atggacatct gtgccggccg gcgcggcgcg
    19021 cgccagggcc tgcaggcgcg ccagcgcatc ggcagtggca gcggacgaca gcggcgcatc
    19081 gtcgcccgag ggcgtgggca cgatccagtc caccaccagg gccgacggcc ccggcgcggg
    19141 cgtgtgcggc tcgctccagc aggtcgcgca atcggcgctg acgaagcgct cgaacaacgc
    19201 agccggccgc ggcgtgtggt cgctgctgca gggctgggcg agggcgccgg ccagcggcag
    19261 cgcgcacagg ccggcagcga tcagggcagg ggttttcatc ggtgggttcc ggtgggcttc
    19321 ctacaattcc accatgctcc cacaagattc gctttccggc gccggtgtgc cgccatcggg
    19381 ccaggccgcg ccgctgctgc gcggtctcaa ccccgaacaa ctcgccgccg tcaccctgcc
    19441 cgccggccat gcgctgatat tggccggcgc cggttcgggc aagacgcgcg tgctgaccac
    19501 gcgcatcgcc tggctgctct cgaccggcca ggccacgccc ggcggcatcc tggccgtcac
    19561 gttcaccaac aaggcggcca aggagatgct cacgcgcctg tccgccatgc tgcccatcaa
    19621 cgtgcgcggc atgtggatag gcaccttcca cggcctgtgc aaccgcctgc tgcgcgcgca
    19681 ccacaagctc gccggcctgc cgcagacttt tcagattctg gacacgcagg accagctctc
    19741 ggccgtcaag cgtttgtgca agcagttcaa cgtggacgag gagcgcttca agcccaagga
    19801 gctggcctac ttttttgccg gctgcaagga agagggcctg cgcccgcgtg acgtgccggt
    19861 gcgtgacgag gacacgcgca agaaggtcga gctgtaccag ctctacgagg agcaatgcca
    19921 gcgtgagggc gtggtggatt ttggcgagct gatgctgcgc agctacgagc tgttgcgcga
    19981 caacgacccg gtgcgcgtgc actaccagcg gcgctttgcc cacatgctgg tggacgagtt
    20041 tcaggacacc aacaagctgc agtacgcctg gctcaagctg ctggcgggcg acatggtcga
    20101 tggccgcctg caaacccggg gcagcgtgat cgccgtgggc gacgacgacc agagcatcta
    20161 cgccttccgc ggcgcgcgcg tgggcaatat gcaggacttc gtgcgcgagt ttggcgtaga
    20221 gcggcagatc aagctggagc agaactaccg cagctacagc aacatcctgg attcggccaa
    20281 cgcgctcatc gcgcacaaca gcaagcgcct gggcaagaac ctgcgcacaa gccaaggccc
    20341 gggcgagccg gtgcgcgtgt acgaggcgaa tagcgacctg gccgaggcgc agtggatggt
    20401 ggacgagatc aggcaactgg tgcagggtgg cacggccacg cgcagcgaga tcgccgtgct
    20461 ctaccgcagc aacgcgcaaa gccgggtcat cgaatcggcg ctgttcaacg ccgccgtgcc
    20521 gtaccgcgtg tatggcggcc tgcgcttctt cgagcgcgcc gagatcaagc acgcgctggc
    20581 ctatctgcgc ctgctggaga acccgcacga cgacaccagc ttcatgcgcg tggtcaactt
    20641 tccggcgcgc ggcatagggg cgcgctcgct ggaatcgctg caggatgtcg cgcgccagat
    20701 cggctgctcg ctgcacgacg ccgtcagcag catcagcggc aaggccggcg ccaacctggg
    20761 cgcctttgtg gccatgatcg acgtgctgcg cgagcagacc caggggcaga acctgcgcgc
    20821 catcatcggg cagatgctgc aggccagcgg cctgatcgag cactatcgca acgaaaagga
    20881 aggcgccgac cggctggaga acctggatga actcatcagt gcggccgaga gctttgtcac
    20941 gcaggagggc tttggcaagg acgccgtggc cctgccgatc gatgaaaccg gcgcgccgct
    21001 cacgcaaagc ccggccagcc aggggctgga cccgaacgcg ccgctgctga accagcccct
    21061 ggtcgatgcc gacaccggtg agaccctgtc gccgttggcc gccttcctga cccacgccgc
    21121 gctggaggcc ggcgacaacc aggcccaggc cgggcaggac gccgtgcagc tgatgaccgt
    21181 gcacgccagc aagggcctgg agtttgactg cgtgttcatc ggtggcctgg aggaggaact
    21241 cttcccgcac cagaacgcca tgagcgacca gggcggcctg gaggaagaac gccgcctgat
    21301 gtacgtggcc atcacgcggg caagagcgcg cctgtatctg agccacgcgc agacccggtt
    21361 gctgcacggc cagacgcgct acaacctcag gagtcgcttc ttcgacgaac tgcccgagga
    21421 atgcctgaag tggctcacgc ccaggcagca ggggtttgcc agttatgctc ctgatatgag
    21481 agctgttggc gcttatcagt cgggcgctag aggccaatct ggcttcaaat cagaggcctt
    21541 caccagcccg cccgtgccgc cgcgcaagga ggctccggcg catggcctgc gcgtgggcct
    21601 ggcggtgttc cacaccaagt tcggcgaggg caaggtgctg gccctggagg gagctggcgt
    21661 cgacgcccgt gcccaggtca actttccgcg ccatggcacc aagtggctgg cgctggcggt
    21721 ggccaagctg accatcgtgg aataacccag cccgcgccgg gccgtgcccg cctgtgcgca
    21781 tggcatcggg gcgctgcctg cgtgcccttg ctgctgtcac gccggcgtca caagcgattt
    21841 ctagactgag cactccaatc catgtttcac aacgcgagga gtgtggctct catgagcaag
    21901 tctgtttccg acaaggtggt tcgcaaccat gccgacaatg ttccgttcag cgccatcctg
    21961 caggccaggc tgtcgcgccg cacggtcgcc cgcggcggcc tgagcgccgc cctggccctg
    22021 ctgaccggca ccgggctggc cgcatgtggc ggcggtgacg gccagggcaa cgcgccggct
    22081 cccgtgccag cgcccggtct caagctcggc tttgagtccc tgcccgcctc catgaccgat
    22141 gcctgcgtgg tgcccgctgg ctacgtggcc catgtgctgg gcccctgggg cacgccgctc
    22201 aacgacaacg ccgccgaatg ggacaaggac ggcaacaaca gctcgacaga cctgctccat
    22261 tccacgggca tgcaccacga tggcatgcac tactttgcgc tggagggcag ctccaccgag
    22321 ggcatcctgg tcgtcaacca cgaatacatc gacgagcagg ccctgcaccc cgccggcccc
    22381 accatggtcg gcggcaagcg cccggccgag gaggtgcgca aggagatcaa cgcccatggc
    22441 gtcgccgtga tgcatatccg caagagcggc ggcagctggg ccatcgtcaa gaactcgagg
    22501 tacaaccgac gcttcacctc cgccacgccc atggccctgg ccggccctgt gagcggcacc
    22561 gactgggtca agacgccgtt ctcgcccacc ggcaggcagg ttcgcggcac caacaacaat
    22621 tgcggcaacg gcacgacccc ctggggcacc tacctcacgg ccgaggagaa ctgggctgcc
    22681 tgcttcgtca acaccgacaa ggccaaccag cccgcgcacc agaagcgtgt cggcgtttcc
    22741 accagcaagg gccgctacca atgggaaacc gctggcgggc acggtagcga agagcagggc
    22801 gagttcgcgc gcttcaacgt cacgccatcg ggcgcggacg ccttgagcga ctggcgcaac
    22861 gaggtcaacg gctttggcta tctggtggag atcgacccct acgaccccgg cagcattgcc
    22921 accaagcgca cggccatggg ccgctttgcc catgaggggg cggcttacgg cctgccccag
    22981 gcaggcaagc cgctggcctt ctataccggc gacgactcgc gcttcgaata catctaccgc
    23041 tatgtgtccg atgccgcctg ggatgctgcc gatgccaggc gcacggatcg cctcgccgtg
    23101 ggcgccaagt acctggacaa gggcacgctt tatgtcgccc gcttcaacga cgatggcacg
    23161 ggcagctggc tgccgctcac gcccgacagc gtgggcaagg acggtacgcg gctgggcagc
    23221 aggtacgcca cgctcgatga aatcctgatc aacacccgcg gcgcggccga tttcatgggc
    23281 gcaaccccca tggatcgtcc cgaatggaca gccgtgcacc cgacgaatgg cgacgtgtac
    23341 ctgacgctga ccaacaacag cagccgcaat gccggcacgg gcacgaacgc ggcgaacccg
    23401 cgcctgaaca acgtcaacgg ccacatcatc cgctggcacg acgacgcggg cgcgaacagc
    23461 ttccgctggg agatcttcgt gttcggctct gacgccgccg cagcagccga caccaaccgc
    23521 tccggcctga gcgcgctgaa ccagctggcc agccccgacg gcattgcctt cgacgcgcgc
    23581 ggcatcctgt ggatacagac cgacaacggc atcgacggcg ggcgcaacaa cgctgtcgcc
    23641 cgggccacca acgaccagat gctggcggtg atccccggcg cgctggccga gcacaagggc
    23701 ccggggccgg ccatcaacgc cgccaaccag ggtgatctgc gccgcttctt cgtcggcccg
    23761 aacgcggccg agatcaccgg ctttgcctgc acgccggact tcaagaccat cttcctgaac
    23821 gtgcagcacc ctggcaactg gcccgcctat ggcagcagtg acgccacgct ggcgcccacg
    23881 ggcacggtgc gcccgcgggc agccaccgtg gtcatccaga aggccgatgg gggtgcgatc
    23941 ggcgtctgac ccgcgcaagc agcatggccc agggagccat gcggtcggcg gtaccgacca
    24001 gggagctgcc acgcccatgc gtggcagctc tgttggcgct tttggcttgc gtttgccccc
    24061 atggcgcgcg caaggtgcgg tggcacaatc ccagcggcgt ggtggcggcc cgttgaacgg
    24121 ctggccatct gcttcatctt tgggtgcggt ttccggaact cctggcgaat ttttttcatg
    24181 ggtcagaggc gacattggac tcccctgtgg ctggcgcacc ggcccgggcg gcgtgggctt
    24241 ggtgtctggc tcgcgctgtg cgtcgcctgg gtggcaatgg cctctctccc ggccctggcg
    24301 gcctgcggcg gtcaggtgcg actgacgcag ccggtggagt tcagcgccca ggagctggcc
    24361 gggtttcgcg ccatggcgcc gctgcgcgtg ctgcccctgg acgcgccccc gatggcgcgc
    24421 cacgacacgc aagatggcta ctccggcatc ggcgtggatg tgtggtgctt catcaccgag
    24481 cgcctggggc tgcgctatga aatcattcag cggcgggacc tgtccatcgc tgaaaaaatc
    24541 cgcctggtgc aggaaggcca ggccgatgtc ctgctgccct tgagcgcgaa cagcgaacgc
    24601 gccgcgcgcg gcatcttcac ccggccgttc tacgaaagcc attacgccgt catcgcgcgc
    24661 aaggactggc gcccggagat ccgcggcctg gccgacctgg cgccctaccg cgtgggcgtg
    24721 ctcaagggcg tggcgtccga gagcctcctc aagacggtga tggcgtccga tcggctggtg
    24781 gtcattgacg acgtgaccag cgaggcgctg tttcaggccc tgcgcgatgg ccgtatcgac
    24841 gtggccgtgt tcagcaaggc ggtttttgaa gaaaagcgct accagcacga gtactttgac
    24901 ctggccgtgg tgcataccct gcacgatgcg ccgcgggtct acggttttta cttccacgcc
    24961 tcgccacgca accagcagat cgtgcaggcg tttgaccgct acctggcggt gctggatgtt
    25021 tccggcgcgg tggccgagca tgaggacggc gagcgccagt tcatccagcg ctatgtgatc
    25081 cagcgcagct ggcacctggt ggtgctgacc gccggcgcgg gggcgctggt gctggtcgtg
    25141 gtgctggcgg cgttgctgta ccgctaccgc cgcctggcgc gcctgctggc ggccagcaac
    25201 gagcacatcc tgcagcagca gcaggctctg caggcggtca accaggatct ggaaaggcag
    25261 agccagaccg acgggctgac gggcctggcc aaccgccgcc agtttgacca tgcgctgctg
    25321 cgcgaacatg cgcgccagca gcgcacgggc gcgccgctga gcctgctgat ggtggatttg
    25381 gaccatttca agcatgtcaa cgaccgctat ggccatgcca ttggcgacga ctacctgcgc
    25441 gccgtggccc gggtgctcaa cacatcggtg acccgtgcca ccgacctggc ggcgcgctac
    25501 ggcggcgagg agttcgtctg cctgctgccc gaaaccgcgg ccaccgatgc ccagatgctg
    25561 gccgagcgca tccgccaggg cgtgagcagc atggccctgc ccaacgagct ggccagtccc
    25621 ccgcacctga cgctgagcat aggcgtcgcc acgctgcttg gcggcaagac ggatgcggcg
    25681 cagctgctgg cccaggccga cgagcagctg tacgccgcca agcatgccgg gcgtgaccgc
    25741 gtgcatgcgg tggtgttgcg ttgacgcggc aagcagcggt ggttggcgcc cgtcatcgcg
    25801 gcgaaggctg gtatccagcg ttcacgctgg ccaatggcat cccgggatcg cggcctttgc
    25861 cgggacggcg gtcagtcctt tgctgcccgg acgatagggc aatggttcag tgcgatggat
    25921 ctgcgcccgg ggccggctcg tcgccgttcg tctgcgtgtc gggcggcgca aagctgtcgc
    25981 ggaaggtgaa caccaccgag gtgaagaaca tcgccgccag catcatcgcc gtagccacca
    26041 tgatgccgcc ggccatgctc gcgccaatgc ccacggcggc cagcagcgtg accagcaggc
    26101 tcatggccag gcccgccgcc aggaacacgc ccagccagga caggccatac accgtgaagg
    26161 caccaaagtt gcgccagcag gcgacgatgc tgaagaacag cgccttcacg ggcggcacgt
    26221 tgtgccagtg caccaggccc ggcgcatgcc agaacagcag cgacagcggc acgtacagca
    26281 gcatggccag ccacatggcg gcctggaagt cgccggactc ggccacctcg cgcgtgagtg
    26341 gcgcgccgcc caggtagacg cgcgcgaatt cgccgccatc gatcagggcc gacagcccca
    26401 tcacgcccag aaagcccacg gcatacatgg cgcccaggct catcatggcg ttcaggcgct
    26461 gcttgccggt gcgaaaggcc accagcatca aggccggtgt gggcgtgcgg ttctggctgg
    26521 cctcggccgc ggcgaccatc atcgccagcg tggccgacgg cagcagcgcc agtgccacgg
    26581 cggggccaac cagcggcagc agcgttgcca gcgacatggc ggccatggtc atgaaaaaca
    26641 gcgcggacag ggccatgggc tggcgccaga acacggtgat gccctggcgt acccattgca
    26701 ggcccgtgcg cgcggggacg atgttcagtt tcatggtgcg gcgactgtac cccagggccc
    26761 gatggccctg gcaggcagga atatcaagga tggacggggt gccgcacacg ctcgcgcagc
    26821 acgcgttcga aatgcgtggg gtcgtggggc ttgagcatgc tggcctcgcg cggcaggtaa
    26881 aagtcccaca ggcgcgacag ccagaagcgc agcgcgccgg cgcgcagcat ggccggcagc
    26941 agctcgcgct cggccgccgt gagcgggcgc actgcctggt aggcggccag cagggcggcg
    27001 gcgcgctgct cgtcgtgccg gccggtgggc agatcgatgc accagtcgtt caggcacacg
    27061 gccaggtcaa aaagccaggt atcgacgccg gcgaagtaga agtcgaagca gccggtgagc
    27121 tcctcgccct cgaacatcac gttgtcgcgg aacaggtcgg cgtgtatcgg cccgcgcggc
    27181 agcgcggcgt aggcgctgcc ggccgccaca tggttctggt aggccagctc ggacgtgagc
    27241 agctcggcct gttccgggcc aatgtgcggc agcaccacgg gcacggtctc gttccaccag
    27301 ggcaaggccc gcagattggg ttgctggcgc tcgtagtcgc gcgcggccag gtgcatgcgc
    27361 gccagcatgt tgcccaccgc ggtgcagtgc acggcctgtg gcgccagctg gctcttgccg
    27421 cgcagcttgg tggccagggc ggctggcttg ccggccacct ggtgcaggat ctcgccgtac
    27481 ttgtccgcct gtgggtcggg cacggggatg ccgtggtgcg ccagatggtg catcaggtgc
    27541 aggtagaacg gcagctgctc ggcgctcagg cgctcgaaca gcgtcagcac atattcgccc
    27601 gcttcgctgg tcacgaagta gttggtgttc tcaatgccgc cctctatgcc gcgcagcgtg
    27661 agcagcttgc ccagctgcag gcggcgcagc agctcgcgcg cctctttgtt tgagacttcg
    27721 gtgtaaacgg ccatggatca gcagaaagaa acgtgcggcg gcgggagggc ggcgcagccg
    27781 ccggtggtgg cggcatgggc gcgggaggtc agaactggcg caggttccac acgcgtggcg
    27841 cggtcaccga gtcggactgg ttgaagttgc cgggccgcgc gcgcgcgcca tcattggcgc
    27901 gcacctcgta ggcgggcagg ggcgtgccgg tcttgggttg caccgtgatg tgctgcgtct
    27961 ggccgcccac gcgcagctca tcgacgcggc tgccggcgtc ttcgaccacg atgcgctcca
    28021 cgcgcggttc gcgccggtca tcggcttttt gctcttgtgt tgatagctgt ccgcgctcag
    28081 tggtattggg ctgagacggg ttttggcttg tattttgtgc cagggcgggg gcgctggcga
    28141 cggccagcag cagaaaggga taaacggcgc gcatggcgcc gattgtaggt cgtggcaggg
    28201 cgcgggtcag cgctgcggcg ggcagccgct ggccgtgcag gccacggtgt actggcttat
    28261 gaaggtgcgg aagttcttgc ccagccggcc catctcggtg cagacgttgc cgcgcgtgtc
    28321 gaaggcaaag ccctggtcgc ccttggcgtt gagcagctcc acgcgggcca ggcccaggct
    28381 ggcgaactgc tgcggctcat accactcgcg ggcgcagatg ttgtggaaga cgaaggtctg
    28441 gtagaccagc tcggtgacct cggccttgtt cagctgcgcg cgcagcacgc cgccgtcgaa
    28501 ctgcgccgcc gtcacgccgc cgtgctggat ggacttgtgc agcagcggcg gcacgttctt
    28561 gaattcgcta tcggccaggg ccagggtgga ggcggcgcag gccagcagtg caacgatgta
    28621 tttcatggcg ggagaatgcg tgaatggctc ggcgcggcgc cccatggcgc cgccgtcgag
    28681 gcccatgatt gtgcaggttt tgccgcgggc ttatgtgcac aatctcccca tgagtgaatc
    28741 caaaaccctg gtgctggtcg atggttccag ctacctgtac cgcgccttcc acgccatgcc
    28801 cgacctgcgc gccgtgccgg gcgacccggc cagcagcccc acgggggcca tacgcggcat
    28861 gatcaacatg ctgcaggcgc tcaagaaaga ggtgccggcc gactacgcgg tctgcgtgtt
    28921 cgacgcgagc ggccccacct tccgcgacga catctacgcc gagtacaagg ccaaccgctc
    28981 ccccatgccc gacgacctgc gcgcgcagat cgctcccatc cacgaggtgg tgcgcctctt
    29041 gggctggccg gtgctggcga ttgccggggt ggaggccgac gacgtcatcg gcacgctggc
    29101 caagacggcc gcggcccagg gcttgcgcgt ggtgatatcg agcggcgaca aggacctgag
    29161 ccagctggtg gacgagcgca tcaccatcat cgacaccatg aacggcaagc gccgcgacgt
    29221 ggccggggtg acggccgagt ttggcgtgcc gcccgcgctg atggtggact tccagaccct
    29281 ggtgggcgac gccgtggaca acgtgccggg cgtgcccaag gtcggcccca agaccgctgc
    29341 caagtggctc atggaatacg gctcgctgga cgcgctggtg gccagcgccg gcgccatcaa
    29401 gggcgtggcc ggcgagaacc tgcgcggcgc gcttgactgg ctgcccacgg cgcgcagcct
    29461 ggtcaccatc aagaccgact gcgagctggc cgatcatgtg gcgggtttgc ctgctcttga
    29521 ttcgatagct gttggcgcag aacagacggg cgaactggcc gatttttatc aaaaatacgg
    29581 cttcaagagc ctggcgcgcg cgctggagcc ccaggcgcag ccggccagcg gcgacctgtt
    29641 tgcccagccc gacgccagca ccgttgccgt ccaggcccag cggcgcgacg tggcctacga
    29701 catcatcctg acctgggcgc agctcgatgc ctggctggcc cgcatccagg ccgcaccgct
    29761 ggtggccata gacaccgaaa ccacctcgct ggacgagctg cgcgccgcca tcgtcggcat
    29821 ctccttcagc gtggagccgg gggccgccgc ctacatcccg ctggcacacg aaggcccgga
    29881 cgcgcccgcg cagctgccgc tggccgaggt gctggcgcgc ctcaaaccct ggctggaaga
    29941 cccggcgcgc ttgaagctgg gccagcacat caagtacgac cgccatgtgt ttgccaacca
    30001 cggcgtcgag gtacgcggct acgcccacga caccatgctg caaagctacg tgctcgaagt
    30061 ccacaagccc cacggcctgg ccagcctggc cgaacgccac acgggccgca agggcatcaa
    30121 ctacgaagac ctgtgcggca agggcgccaa gcagatcccg tttgcccagg tggcggtgga
    30181 caaggccgcg gcctactcgt gcgaggactc ggaccagacc ctggacgtgc accgcgtgct
    30241 ctggccgcag ctcgcggccg aagagaagct gcgcgccatc tacgagctgg agatcgcctg
    30301 cagcgagacg ctgttccgca tagagaggaa cggcgtgctg atcgacgccc agcagctggc
    30361 cagccaaagc cacgccctgg gccagcgcat cctgcagctc gaaaaagagg cctacgacat
    30421 cg
//
LOCUS       DAOMTQ010000013        30267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_125_length_30267_cov_25.730802, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000013 DAOMTQ010000000
VERSION     DAOMTQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 30267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30267
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_30267_cov_25.730802"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1890)
                     /locus_tag="PK518_02575"
     CDS             complement(121..1890)
                     /locus_tag="PK518_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HRM47823.1"
                     /translation="MSQTHFSSLASSPQALAAAPSGPAWLAFLRRGLQRVDGWFASPL
                     SAQQLVLRLSVYLVLAANWPLWLQLVQIGGAPSIYLRSVAEMALLIVCGMVALLAFTA
                     WTRGMRLLWWLVLLVAALAQYYMLTYSVVMDPGMAANVLQTDAREAADLLSPRLLGAV
                     LAVMVLPTWWLVRVRIPPMRPLRQLGRNLALLALALAVAAGMVALNARHLAPLMRNHP
                     QLRYLMNPLASLYSTSVALARPVFARGRTLVPMTQGAALGASYASATKPLLFVLVVGE
                     TARADHFGINGYGRDTTPELARRGVMSWREVRSCGTSTLASLPCMFSPLGKSAFEARD
                     NEYENLLDVAQAAGLAVLWLDNQSGCKGVCERVPHANAADGLGAQERNRLCDGGECRD
                     EAMLQGLDERLAAMPEGQRQRGVLLVLHQMGSHGPAYYKRSADGAKRFLPECRTEVLA
                     NCAHGELVNAYDNSIAATDAFLGQTIAWLQGQSARYDTGLLYLSDHGESLGEYGLFLH
                     GMPYAMAPDVQKHVPMVAWLDGGLLRRGRLSSSCLSAGTDAPLTHDNLYHSMLGLLDV
                     QSPTYRRPMDAFDGCRGAALALK"
     gene            complement(1939..2637)
                     /locus_tag="PK518_02580"
     CDS             complement(1939..2637)
                     /locus_tag="PK518_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM47824.1"
                     /translation="MPSWTLRRLWALTLLFFVLLVLWDATGFDLPLARWSGSPQGFAL
                     RQQPLFVLLSHELPRMAGAALLLALLVGVFKPWGFLCRLAPGERWQLWLSIAGGALAI
                     TVLKRLNHTSCPWDLAEFGGVARHVSHWLWGVRDGGAGHCFPAGHASTAFAYLAGWFV
                     LRRSAPRAAGRWLLGALLAGMLLGLAQQMRGAHYMSHTLWTAWICWAWGLALETALQV
                     HRHGRAVVANLNQA"
     gene            2753..3421
                     /locus_tag="PK518_02585"
     CDS             2753..3421
                     /locus_tag="PK518_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM47825.1"
                     /translation="MRVLLVEDDEALSQAVCGYLRAKAFVVDAVPSLAQAGAALHAAQ
                     YAAVLLDLHLTDGDGLSLLPQLRGLKERPIVIVLTARDQVSDRIHGLDAGADDYLVKP
                     YDPGELLARLRAVERRRSTAQLPVLHLGELQIDLSREQVRRAGVPMALTQKEWALLRV
                     LATRPDHLHTRERLQDALYGFDDDTGSNTLEVFISRLRRKLGREHIETVRGLGYRLVR
                     GNAA"
     gene            3418..4839
                     /locus_tag="PK518_02590"
     CDS             3418..4839
                     /locus_tag="PK518_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM47826.1"
                     /translation="MSAGPNAERAQSLAGRLARTLILWVGGVWLLCVLGVAWYTDSEI
                     NHNFDNEIVEVAHRMFDIALQHLDQAPARAADAGPLILPSPGSFADADVLYQLVAPGG
                     QVLARSAEARPEQFDVPLAQGFANTPAWRVYTLIHPTRTIYLQVADPLQERREAANRT
                     LTGLILTMLAMLPPLALLLRRIARRELRMLDQLTHEISLRGGQLLTPIALAGLPRELR
                     SVEDHVNRLLERLAHALDVERALAANAAHELRTPLAAARLRLQTALEHGLSQGDVQAA
                     INALHTLGLRTEKLLQLSRAESSAPFARQPVDLGQLATTVAQEFWQDDVAVDRLDLVL
                     DYAGPGPIALGDFDALAIALRNLVENALHYAGSSAVELIVGPGSMLTVRDHGPGVDGA
                     RLQALQQRHVRDSAERAGYGLGLSIVATIAAKHGAGLTLTSPLPGESGGFEARLRLGA
                     ATAPAHASSPAAAPPFAPESPPR"
     gene            complement(4769..5188)
                     /locus_tag="PK518_02595"
     CDS             complement(4769..5188)
                     /locus_tag="PK518_02595"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRM47827.1"
                     /translation="MKIEIAEHKKLAHSMELDVRWGDMDAMGHVNNAVYFRYMESVRV
                     EWMRAVGCEPSPKGQGPVIVNAFCNFYRQLEYPARLLLKLYVSDPARTTFETWTTIER
                     LDQPGVLAAAGGATVIWVDFPAQKAVPLPDWMRALVL"
     gene            5336..7042
                     /locus_tag="PK518_02600"
     CDS             5336..7042
                     /locus_tag="PK518_02600"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HRM47828.1"
                     /translation="MTNEEILSTYGPREAMEYDVVIVGGGPGGLATAIRLKQLAAEKG
                     QEVSVVVLEKGSEPGAHILSGAIMDPKAITELIPDWKAKGAPLNQPVTDDAMVFLGES
                     SSFRTPNFLLPGCFQNHGNYIVSLGNVVRWMAEQAEGLGVEIFPGFAAAEVLYNDDGS
                     VKGVATGNMGISKEGEPTGDFQLGMELHAKYTIFAEGARGHLGKQIIAKYQLDAESDP
                     QTYGIGIKELWEIDPKRHQPGFVLHTAGWPMDNNTYGGAFLYHLEDNKVTLGFITGLD
                     YSNPYLSPFEEMQRWKLHPNIRWYLEGDEAKGIKPAKRLGYGARAITAGGLLSLPKFV
                     FPGGALVGCDAGFLNVSRIKGSHAAIKTGMLAAEAAFDAIGAGRQHDELTAYPKAFEA
                     SWLYTELNKSRNFKAWFKKGLVMASAMNGIEQFALRGHIPWTLRRDTPDHAYLKPAAE
                     CKPIVYPKPDGKLTFDRLSSVFISNTNHSENQPAHLTLKDASVPVNVNLAKYAGPESR
                     YCPAGVYEFVPDEAQGGNAQRLQINAQNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYS
                     GM"
     gene            7267..8235
                     /locus_tag="PK518_02605"
     CDS             7267..8235
                     /locus_tag="PK518_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM47829.1"
                     /translation="MKSLSWAPLGAFALAMATLAPVAHAQQKSVAVTAIVEHPALDSV
                     RDGVQAALKAAGYESGKNLKWQYQSAQGNTGTAAQIARKFVGDNPDAIVAIATPSAQA
                     VVAATKTVPVVFSAVTDPVAAKLVPSWEPSKTNVSGVSDLLALDKQMDLVKQVVPNAK
                     RVGMVYNPGEANSVVVVKELQKLLPTLGMTLVEAAAPRSVDVSSAARSLIGKVDVIYT
                     NTDNNVVSAYESLVKVGQDAKIPLIASDTDSVKRGAIAALGINYRDLGEQTGRMVVRI
                     LKGEKPGDIKPETSSKLELFVNPGAAEKQGVKLSEQLVKSAAQVVQ"
     gene            8317..9201
                     /locus_tag="PK518_02610"
     CDS             8317..9201
                     /locus_tag="PK518_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM47830.1"
                     /translation="MSLFSLLGAVEIGLIFSLVALGVYISFRLLRFPDLTVDGSFPLG
                     GAVCAILISTGTNPWLATLAATAAGALAGLVTGWLNVKLKIMDLLASILMMIALYSVN
                     LRIMGGPNVPLINDTTLFTLLQPESIPDYVARPMVLFVVVVLAKLALDWFFATERGLA
                     IRATGSNARMARAQGVNTGAMVLLGMAISNALVGLAGALFAQTQGGSDISMGIGTIVI
                     GLAAVIVGESILPSRRIVYATLAVIVGAIVYRFFIAAALNSDFIGLKAQDLNLVTAAL
                     VTVALVIPQLKRKLSSRK"
     gene            9225..10019
                     /locus_tag="PK518_02615"
     CDS             9225..10019
                     /locus_tag="PK518_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM47831.1"
                     /translation="MLSAQDLFITFNPGTPIETRALRGMSLEIPAGQFVTVIGSNGAG
                     KSTFLNAISGDLGVDTGRIAIDNLDVTRRPVWERAERVARVFQDPMAGTCEDLTIEEN
                     MALAQQRGTRRGLRGAVKQAERAMYRERLATLGLGLENRLTDRIGLLSGGQRQAVSLL
                     MAALQPSRILLLDEHTAALDPRTADFVLQLTARIVTENKLTTMMVTHSMRQALDVGQR
                     TVMLHQGQVVLDVSGEERARLDVPDLLQMFEKVRGEKLADDALLLS"
     gene            10146..11621
                     /locus_tag="PK518_02620"
     CDS             10146..11621
                     /locus_tag="PK518_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRM47832.1"
                     /translation="MSASDSTSGNHLHRALKARHLSMIAIGGSIGTGLFVASGATISQ
                     AGPGGAVLAYMVVGLMVYFLMTSLGEMAAYLPTSGSFATYGARYVDEGFGFALGWNYW
                     YNWAVTIAVDLVAAQLVMAYWFPGVDGVLWSAVFLAIMFGLNAISVRGFGEAEYWFAA
                     IKVATVIVFIGIGVLMILGIMRGGAPEGLWGNLALWTQDDAPFAGGFAALIGVAMIVG
                     FSFQGTELIGIAAGESEDPARNIPRAVRQVFWRILLFYVFAILVISLLIPYTDPQLLK
                     NDVGDIAVSPFTLVFQRAGLLSAAAVMNAVVLTSVLSAGNSGMYASTRMLYTLACQRM
                     APRIFARVSANGVPVMALIATTVVAALCFLTNIFSPQAVYIWLLNLSGMTGFIAWLGI
                     AISHYRFRKGYVAQGLQLSALPYRTTSFPFGPIFAFVLCMTITLGQNYQAFLQERIDW
                     TGVVATYIGLPLFLVIWFGYRLVKGSRIVPYAKMQVKPEAY"
     gene            complement(11651..12106)
                     /locus_tag="PK518_02625"
     CDS             complement(11651..12106)
                     /locus_tag="PK518_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM47833.1"
                     /translation="MDRLDRKILSVLQGNARASLQDIGAAVGLSPSPCWGRIKKMEES
                     GVIEGYTVRLNAQALDLGDTVLVQVTLDSHSDNTLEKFGEVLASIPEVVEAHLVSGDY
                     DYLLRVVVKDTRDYERLLREKLYKIKGIRHSRSSFVLRTLKKADLPLGV"
     gene            12459..13577
                     /locus_tag="PK518_02630"
     CDS             12459..13577
                     /locus_tag="PK518_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRM47834.1"
                     /translation="MTEQTVETGRRKLLKGAAVGAGALSVPMISTAQTTTMRFQSTWP
                     AKDIFHEFAVDFAKKVNDMAGNRLKIEVLPAGAVVPAFQLLDAVAKGTLDGGHGVVAY
                     WYGKTQAVALWGSGPAFGMDANMVLAWHNYGGGKELLEEIYKSLNLDVVYKLYGPMPT
                     QPLGWFKKPVSKVEDLKGLKFRTVGLAVDVFNELGVAVNPLPGGEIVPALDRGLLDAA
                     EFNNASSDRVLGFPDVAKNCMLQSFHQSSEQFEVLFNKTRYDALPAELKSIIDYATQA
                     ASADMSWKAADRYAKDYAEMQAKQGVKFYKTPDAILRAQLAAWDKVTAKKAEENPMFK
                     KVLESMRAFAQRSGRWQNDTTIDYRMAWNHYFAPKKKG"
     gene            13698..14324
                     /locus_tag="PK518_02635"
     CDS             13698..14324
                     /locus_tag="PK518_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRM47835.1"
                     /translation="MQKLLLRIDAVSTFIGKAAAWLCVPLTLLICWEVFSRYALNKPH
                     AWVLDAQIMLYGTMFMLAGAYTLAKNGHVRGDVLYGFFSPRVQAGLDLALYGLFFLPG
                     VLAMTWAGWNYAQESLAIREQTFSAEPLPLYPFKFVIPLAGAALLLQGLAEIARCVIC
                     LRQGAWPRREDDVEEVDVEKLKRMVHIEGTMYEASQPTSTPGQQGGKP"
     gene            14321..16303
                     /locus_tag="PK518_02640"
     CDS             14321..16303
                     /locus_tag="PK518_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRM47836.1"
                     /translation="MKLRKELWFGLSIMALVVGGAAWGLLSTPTITNGHLGLLMLSLV
                     VVAIMLGFPTAFTLMGMGMIFTWLAYDRDITRSLDLMVQAAYKSMVNDVLIAIPLFVF
                     MGYLVERANLIEKLFKSLHLAMARIPGSLAVATLVTCAIFATATGIVGAVVTLMGLLA
                     LPAMLRAGYSIPLSAGAITAGGCLGILIPPSVLLILYGATAGVSVVQLYAGAFFPGIV
                     LAGLYVVYVVIVAKIKPAWAPPLSDGERQVDLPALTAALPAQTGQHAVVRLLDALKGR
                     RNAQVPLAHVLRQLALVVLPGLLFLVLVVSSWNAVTTVKVEETFDIQPIGLGESSSPA
                     SGLQEPPGDGGLQEPPAEGSLQEPPAEQAEENQPDTALAEPPGAEPEVQAQGQDGAAV
                     AEPPAAAAAEAPAQAETQPAPTWWWVLFSLLGAVTALFYLFLSFARLEIFKMLLSSFF
                     PLMMLILAVLGSIVFGLATPTEAAAMGALGGFLLAAAYRQLTMDVLRESVYLTAKTSA
                     MVCWLFVGSAIFSAAFALLGGQEVIEKWVLSMNLTKVEFMVLSQIVIFLLGWPLEWTE
                     IIVIFMPIFIPLLSHFGVDPLFFGLLVALNLQTAFLSPPVAMSAFYLKGVAPPYVTLN
                     QIFLGMLPFMGIQILAIVLLYAWPSIGLWLPQLLYK"
     gene            complement(16326..17378)
                     /locus_tag="PK518_02645"
     CDS             complement(16326..17378)
                     /locus_tag="PK518_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM47837.1"
                     /translation="MAEEVVTDKNGLVYPCGVAPAVGDAKELAPGVWWLRMPMPFALD
                     HINLWALRDGDGWALVDTGIWSTDTAGAWRQLFAGVLDGPVTRVFVTHMHPDHIGMAG
                     WLTRKFACRLWISRLEYLSCRALSVDTGREAPDDAIAFCRRAGWGEEAIETYRTRFGG
                     FGKLIYALPDSYRRLRDGEEIAIGAQRWRVVVGAGHSPEHVCLYCPELKLLISGDQVL
                     PGISSNVSVFPAEPDANPLADWLESLEKLERSVPGDVLVLPSHNQPFRGLHRRIAALR
                     DSQHRALDRLREALRQPQRVVDVYGALFSRPVTADNMQMSLATGESVAHLNYLVARGE
                     ARVRMGKEGCAWYQMV"
     regulatory      17472..17557
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      17577..17685
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            17796..18860
                     /gene="gcvT"
                     /locus_tag="PK518_02650"
     CDS             17796..18860
                     /gene="gcvT"
                     /locus_tag="PK518_02650"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRM47838.1"
                     /translation="MVPFAGYSMPVQYKSGLMAEHQHTRQAAGLFDVSHMGQLKLIGA
                     DAAAALESLMPVDVQGLAEGKQRYGLLLNDEGGVIDDLMFFNQGNDTWFLIVNGACKL
                     TDIAHIETHIGNRCQIVTLPTQGLLALQGPQAVTALSRLVPGVEKLVFMSGASFAWNG
                     AELFITRSGYTGEDGFEISVPGEHTETLARALLAQPEVKPIGLGARNSLRLEAGLCLY
                     GNDIDSTTTPPEAALNWAIQKVRRSGGERAGGFPGAAKVLAQIDNPASLSKKRVGLIA
                     KERVPVREPAVLENLDGQHVGHVTSGLLSPTLNQPIALAYVQPEYAQPGTEVFAMVRG
                     KPVAMVVSPTPFLPPRYHRG"
     gene            18941..19315
                     /gene="gcvH"
                     /locus_tag="PK518_02655"
     CDS             18941..19315
                     /gene="gcvH"
                     /locus_tag="PK518_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRM47839.1"
                     /translation="MSIQFSKDHEWVNSADANAAVVGITVHAQDALGDVVFVDLPAVG
                     ATFAQGDVAGVVESVKAAADVYMPVSGEVVEVNEALRDDPSLANSDPMNAGWFFKVKL
                     SAPAELDALMDEAAYNAFAASA"
     gene            19442..22324
                     /gene="gcvP"
                     /locus_tag="PK518_02660"
     CDS             19442..22324
                     /gene="gcvP"
                     /locus_tag="PK518_02660"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRM47840.1"
                     /translation="MPALPLSALENHAEFLPRHIGIDSADEALMLSVIGEASRQSLVA
                     SIVPRSIARASSMDLPAPCTEAEALAELKTIAAKNQIKKSFIGQGYYGTHTPGVILRN
                     ILENPAWYTAYTPYQAEISQGRMEALVNFQTMVTDLTGMDIANASMLDEATAAAEAMT
                     LAKRSVKARGNSIIVSGDCHPQTIEVIQTRAAPLGFTVKLVQSEAEWNAAIASDDYFA
                     ALQQYPSSSGWLADWQASSDAIHAKGAAFILAADLLALTLLKAPGEMGADIVIGTTQR
                     FGMPMGAGGPHAGFMACKDAFKRSMPGRLVGVSVDVHGQPAYRLALQTREQHIRREKA
                     TSNICTAQVLPAVIASMYAVYHGAAGLTRIAQRVARLTAILAAGLKQLGLPAEHASAF
                     DTLAVDLGSSAAADATLARALALGANLRRLDAQRLAVSLDETTTRADLALLWSAFAPN
                     AAQPSVEALEGAASLIPAALVRASSFLTHPVFNTHHSETAMLRYIRQLSDKDLALDRT
                     MIPLGSCTMKLNATSEMIPITWPEFAHIHPFAPAEQLAGYAELDALLQRWLCAATGYA
                     GVSLQPNAGSQGEYAGLLAIKAYHEAQGHAHRNICLIPSSAHGTNPASAQMVGMQVVV
                     TKCDENGNVDMADLQAKCEQHSQNLACVMITYPSTHGVFETQVKELCQIVHSHGGRVY
                     VDGANMNALVGVAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVVEDLVPYLPGH
                     GTSGNASKVGAVSAAPLGNAAVLPISWMYIRMMGAEGLTAATEVAILSANYISKRLAE
                     HYPTLYASANGHVAHECILDLRQLKESSGVMAEDVAKRLIDYGFHAPTLSFPVANTLM
                     VEPTESEHLAELDRFVDAMIAIREEIRQVETGAWPQDDNPLKNAPHTAESLLAGDWSH
                     PYTREAAAFPVKSLRGAKYWAPVGRVDNVYGDRNLFCSCVPMSELA"
     gene            22350..23330
                     /locus_tag="PK518_02665"
     CDS             22350..23330
                     /locus_tag="PK518_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRM47841.1"
                     /translation="MASENLKPEKLAVLIDADNTTSSCAQGLLEEIAKYGIASVKRIY
                     GDWSSPLLSGWRSILLKHALVPIQQFAYTKGKDATDMGLIIDAMDLLYSGHFDGFCLV
                     SSDSDFTPLASRIRASGRMVYGFGREKTPEAFRQACDRFFYIENLGEAGKGKDDIVAV
                     PNAVMAASPDIAKPAAKPRQMDGTTKNLLYKSIKDATDETTGWAFVGKIGNVISETRP
                     DFDSRTYGYAKLSGMLRELRGLQFRTDEANRMYCRKIPFGDLIKLLDEAFNKFKNAKG
                     WASLDVTGKYVKPRWNWEEYGFESFTDLLSKVDHVEIANDSMRMQVSIAP"
     gene            23436..23834
                     /locus_tag="PK518_02670"
     CDS             23436..23834
                     /locus_tag="PK518_02670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRM47842.1"
                     /translation="MRTTLTLLAALFLTPVALAQSTPVTTASGVVYESLHDGTGISPK
                     AGDTVKVHYRGIAGDGKEIDSSYKRGEPAEFPVNRVIPCWREGLQLMKEGGKAKLSCP
                     PATTYGMRGSSALIPSNTTMQFEIELLSVR"
     gene            23858..26032
                     /locus_tag="PK518_02675"
     CDS             23858..26032
                     /locus_tag="PK518_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM47843.1"
                     /translation="MGQPDLSFLLRGMEPPQPPGDATSPAGMPERRSPERASGAVAQL
                     IAELRASQAELETQNKVLRYSQAAAESASERFEALFANVPLSLMVLDAHDMVVQANSM
                     AQRSFQPQENDRPLIALMPFVGAEHAPRVQAAFAAATQQGTAEATEVVFHIDDERRIT
                     GDLHIARIESHSDDGTVLTQFLCAVIDQGPLLAERQALQRSAAELQERNAQLHAGERR
                     LEAVINSALDAIICVDQSEHITVFNPTAAMLFQCAASDALGSPLSRFLPDAQQVLAFA
                     QLTTQAALGEMTALTAGGNELAVEVNVSFERHAEGETTTVFARDLTGKKREEARRNAL
                     EAQLRESHKMQAVGTMAGGIAHDFNNILHAILGNVELAKADCTPGSALHESLLEIDKA
                     GRRARDLVRQILTFSRNEAPRRAAVALAEVAHDTERLLRVTLPPEIELSLHLQPELPP
                     VLADATQVEQALLNLCTNAVHAIGNRRGRIQVDGVLVQPDQHQRENLGLAAGDYVALR
                     VQDDGPGMDEATLPRIFEPFFTTKPVGQGTGLGLAVVHGVMRTHGGAIDVHSAPGRGS
                     SFTLYFPVATASPQEGAATATPPAPAPEPAPPASRRQRHVMYVDDDEALVFLVRRLLR
                     RRGYEVSGFTDPHEATDALRAAPGRYDLLVTDYNMPGYSGLDLVRAARAIRPDLPVAL
                     ASGYVTAEIEQAALAEGARALIHKPNDVAELCATVDRLAHER"
     gene            26022..26690
                     /locus_tag="PK518_02680"
     CDS             26022..26690
                     /locus_tag="PK518_02680"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM47844.1"
                     /translation="MSDEALAGLACLHADDDLLVLDKPPGLLCVPGRGPDKQDCLSTR
                     AQALWSDALIVHRLDQPTSGLVLMARGLPMQRALSRIFAERRVHKRYQAVVAGLPQPD
                     DTADDEGWSAIALPIAADWERRPLRVVDAQSGQPSLTRWRVLDVDRALGCTRLELEPV
                     TGRTHQLRVHLAALGHPILGDMLYANAAQQASAPRLLLHACTLEFAHPLHGRRCSYIS
                     AAPF"
     gene            26724..27506
                     /locus_tag="PK518_02685"
     CDS             26724..27506
                     /locus_tag="PK518_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM47845.1"
                     /translation="MTQQLFILTGASRGMGLAMAQQLLQPGHTLICMARNSNSQLTAA
                     VPAGAMLEQWTVDLSEPAPAAQQLHAWLAGQSAGRYASATLINNAGVIPRIAPLSACA
                     SAQDMADQARALRVGLEAPMLLTAAFLGATEGWGAPPHAVARKVLNISSGLGRRAMAS
                     QAAYCAAKAGMDHFTRCLALDEAAKAHGASVCSLAPGVIDTDMQIQLRGASAADFPDV
                     QNFAALKSGGMLTTPAAAAARVLAWLERADFGSNPVADVRDA"
     gene            27602..28786
                     /locus_tag="PK518_02690"
     CDS             27602..28786
                     /locus_tag="PK518_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HRM47846.1"
                     /translation="MTREVVVVSGVRTAIGTFGGALKDTPTVDLGALVVKEALARAGV
                     EGKDVGHVVFGHVVNTEPRDMYLSRVAAINGGCQKETPAMNVNRLCGSGLQAIVSASQ
                     GILLGDYDIAIGGGAENMSRAPYASLATRFGARMGDSKMVDMMVGALHDPFHTIHMGV
                     TAENVAAEHGITREMQDALALESHRRAEVAWAEGRFKDQIVPVMLKSRKGEVAFDKDE
                     HFRPGAKLEDFQKMKAVFLKENGTVTAGNASGINDAAAAVLLMEAGAAKARGAKPLAR
                     LVGYAHAGVEPKTMGIGPVPATQQLLQKLGMKIDQMDVIEANEAFAAQACAVTKGLGA
                     DPAKVNPNGSGISLGHPIGATGALITVKALYELQRIQGRYALVTMCIGGGQGIAAVFE
                     RM"
     gene            complement(28866..30098)
                     /locus_tag="PK518_02695"
     CDS             complement(28866..30098)
                     /locus_tag="PK518_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRM47847.1"
                     /translation="MAPRPTPWSLAHPLWLCPFRPFFALAMLSAWLLMGLWGGFLLLG
                     WPLPVVPGGPFVWHAHELLLGFVLAALAGFVLTAVPEFTGTPPFAAGPVRQLVLLWLL
                     GRLAFWSSGWWPGPALALSALAHLGLLAGLMALLAPRLWRDPDRRHLSFLWTLLALAA
                     VVAALYFDALRGISPARWLHVLVGVFMALVVVAMSRISMSIVNASIDELAARQGEDLA
                     PYLARPPRRKLALLTIGLFTLAEFWQAGGPVSGWLALAAACALLNLLNDWHVGRPLLR
                     RWPLMLYAVYLLMAGGYALIGVAVLTGAFSPNAGLHLLTTGVLGLSIYVVICIAGYTH
                     SGLEKGARPWVPLGALLLGASALLRVGAYAGQAPAMMGAAALLWGAAFLLQSAHMLPV
                     FWRPRADGASGCAGITTS"
BASE COUNT         5398 a        10546 c         9613 g         4710 t
ORIGIN      
        1 atgcagccca accccgagtt ctatctgatg ggccgcatgc cgcgcgagaa gaaggcctga
       61 gccaattact acacttttta tagctgtttg cgcttatcca ctaagcgcta cagccatttt
      121 ttacttcaaa gccagcgccg cgccacggca gccgtcaaag gcatccattg gccgccggta
      181 cgtgggcgat tgcacatcca gcaggcccag catgctgtgg tagaggttgt catgcgtcag
      241 cggcgcgtcg gtgccggcgc tcagacagct ggacgacagc cggccgcgcc gcagcaggcc
      301 gccgtccagc caggcgacca tgggcacatg cttttgcaca tcgggcgcca tggcataggg
      361 catgccgtgc aggaacaggc cgtactcgcc cagcgactcg ccatggtcac tcaggtacag
      421 caggccggtg tcataccttg cagactggcc ctgcagccag gctatcgtct ggcccagaaa
      481 ggcgtcggtg gcggcgatgg agttgtcata ggcgttcacc agctcgccgt gggcgcagtt
      541 ggccaacacc tctgtgcggc attcgggcag aaagcgcttg gcgccatcgg ccgagcgctt
      601 gtaatacgcc ggcccatggc tgcccatctg gtgcagcacc agcagcacgc cgcgctggcg
      661 ctggccctcg ggcatggccg ccaggcgctc atccaggccc tgcagcatgg cctcgtcgcg
      721 gcattcgcca ccgtcgcaca ggcggttgcg ctcctgcgcg cccaggccgt cggcggcgtt
      781 ggcgtgcggc acgcgctcgc acacaccctt gcagccggac tggttgtcca gccacagcac
      841 ggccaggccg gcggcctggg ccacgtccag caggttttca tattcattgt cacgcgcctc
      901 gaatgcgctc ttgcccagcg gcgagaacat gcagggcagc gaggccagcg tgctcgtacc
      961 gcaggatcgc acctcgcgcc agctcatcac gccgcgcctg gccagctcgg gcgtggtgtc
     1021 gcggccataa ccgttgatgc cgaaatggtc ggcgcgcgcg gtctcgccca ccaccagcac
     1081 gaacagcagc ggcttggtgg cagatgcata ggaagcgccc agtgccgcgc cctgcgtcat
     1141 cggcaccagg gtgcggccgc gcgcaaacac cggccgcgcc agcgccacgc tggtcgagta
     1201 caggctggcc agtgggttca tcaggtagcg cagctgcggg tggttgcgca tcagcggcgc
     1261 cagatggcgc gcattgagcg ccaccatgcc tgcggccacg gccagcgcca gggccagcag
     1321 ggccaggttt cgccccagct ggcgcagcgg ccgcatgggc gggatgcgca cgcgcacaag
     1381 ccaccaggtc ggcagcacca tcacggccag cacggcgccc agcaggcgcg ggctcaatag
     1441 atcggccgcc tcgcgggcat cggtctgcag cacgttggcg gccatgccgg gatccatgac
     1501 cacgctgtag gtcagcatgt agtactgcgc cagcgccgcc accagcagca ccagccacca
     1561 cagcagccgc atgccgcgag tccaggcggt gaatgccagc aaggccacca tgccgcagac
     1621 gatgagcagc gccatctcgg ccaccgagcg caggtagatg ctgggcgcgc caccgatttg
     1681 caccagctgc agccacagcg gccagttggc cgccagcacc aggtagacgg acaggcgcag
     1741 caccagctgc tgggccgaca aggggctggc aaaccagcca tccacgcgct gcaggccgcg
     1801 gcgcagaaac gccagccagg ccgggccgga cggcgccgcc gcaagggctt ggggactgga
     1861 agccaggctg ctgaaatggg tctgagacat gggccgccat gctcggcccc gcctctgaaa
     1921 cctggctgaa gaccgggttc aggcttggtt caggtttgca acaacagccc tgccatgccg
     1981 gtgcacctgc agcgccgtct ccagcgccag gccccaggcc cagcagatcc aggccgtcca
     2041 cagcgtatgg ctcatgtagt gcgcgccgcg catctgttgc gccaggccca gcagcatgcc
     2101 ggccagcagc gcccccagca gccaccggcc ggcagcgcgc ggtgcactgc ggcgcaatac
     2161 aaaccagcct gccagatagg caaaggccgt ggacgcatgg cccgccggaa agcaatggcc
     2221 cgcgcccccg tcgcgcacac cccaaagcca atgcgagaca tggcgcgcca cgccgccaaa
     2281 ctcggccaga tcccaggggc agctggtgtg gttcaggcgc ttgagcacgg tgatcgccaa
     2341 ggccccaccg gcaatggaca gccacagctg ccagcgctcg cccggcgcca ggcggcacag
     2401 aaacccccag ggcttgaaca cccccaccag caacgccagc aacagcgccg cgccggccat
     2461 gcgcggcagc tcatgcgaca gcaacacaaa gagcggctgc tggcgcaagg caaagccctg
     2521 gggcgagcca gaccagcgcg ccaggggcaa atcaaaaccc gtggcgtccc acagtaccag
     2581 cagcacgaag aacaacagcg tcagcgccca caagcggcgc aaggtccagg aaggcatggc
     2641 aagacccggc aaagcctgca accgtagacc actggcctga acgcagcatg aacgggccag
     2701 gccgccgcgc gggcctacac tttgcagtcc tgtgactgcg acgaggactg gcatgcgcgt
     2761 gctgttggtg gaagacgatg aggccctgtc ccaggccgtc tgcggctatt tgcgcgccaa
     2821 ggcctttgtg gtggatgccg tgcccagcct ggcccaggct ggtgccgcgc tgcatgcggc
     2881 gcagtacgcg gctgtgctgc tcgatctaca cctgaccgac ggcgacggcc tgagcctgct
     2941 gccgcagctg cgcggcctca aggagcggcc catcgtcatc gtgctcacgg cgcgcgacca
     3001 ggtcagcgac cgcatccatg gcctggacgc cggggccgac gactacctcg tgaaacccta
     3061 cgaccccggc gagctgctgg cgcgcctgcg cgccgtggag cgccggcgca gcacggccca
     3121 gctgccggtg ctgcacctgg gcgagctgca gatcgacctg tcgcgcgagc aggtgcgccg
     3181 cgccggcgtg cccatggccc tcacacaaaa ggaatgggcg ctgctgcgcg tgctggccac
     3241 ccggcccgac cacctgcaca cgcgcgagcg cctgcaggac gccctgtacg gctttgacga
     3301 cgacaccggc agcaacacgc tggaggtctt catcagccgg ctgcgccgca agctcgggcg
     3361 cgagcatatc gagaccgtgc gcggcctggg ctaccggctg gtgcgaggca acgccgcatg
     3421 agcgccggcc caaacgccga gcgcgcacaa agcctggccg ggcgcctggc gcgcaccctc
     3481 atcctgtggg tgggcggcgt gtggctgctg tgcgtgctgg gcgtggcctg gtacaccgac
     3541 agcgagatca accacaactt cgacaacgaa atcgtcgagg tcgcgcaccg catgttcgac
     3601 atcgcgctgc agcacctgga ccaggccccg gcgcgagccg ccgacgccgg gccgctgatc
     3661 ctgcccagcc cgggctcgtt tgccgatgcc gatgtgctct accagctggt cgcgcccggc
     3721 ggccaggtgc tggcgcgctc ggccgaggcc aggcccgagc aattcgacgt gcccctggcc
     3781 cagggctttg ccaacacccc cgcctggcgc gtgtacacgc tgatccaccc cacgcgcacc
     3841 atctacctgc aggtggccga cccgttgcag gagcggcgcg aggccgcaaa ccgcacgctg
     3901 accggcctga tcctcaccat gctggccatg ctgccgccgc tggcgctgct gctgcggcgc
     3961 atagcgcggc gcgaactcag gatgctggat cagctgacgc acgagatcag cctgcggggc
     4021 ggccagctgc tcaccccgat tgccctggcc ggcctgccgc gcgagctgcg cagcgtggaa
     4081 gaccatgtga accgcctgct cgaacgcctg gcccatgccc tggacgtgga gcgcgccctg
     4141 gcggcgaacg ccgcgcacga gctgcgcacg ccgctggccg ccgcgcgcct gcgcctgcag
     4201 acggcgctgg agcatggcct gagccagggc gacgtgcagg ccgccatcaa cgccctgcac
     4261 acgctgggcc tgcgcaccga gaagctgctg cagctgtcgc gcgccgagtc gagtgctccc
     4321 tttgcgcgcc agcccgtgga tctgggccag ctggcaacca cggtggcgca ggagttctgg
     4381 caggacgatg tggccgtgga caggctcgac ctggtgctgg actacgccgg gccggggccc
     4441 atcgccctgg gcgacttcga cgcgctggcg attgcgctgc gcaacctggt ggagaacgcg
     4501 ctgcactacg ccggcagcag cgccgtggag ctcatcgtcg gccccggcag catgctcacc
     4561 gtgcgcgacc atggcccggg cgtggacggc gcgcgcctgc aggccctgca gcagcgccat
     4621 gtgcgcgaca gcgccgagcg cgcgggctac ggcctgggcc tgtccatcgt ggccaccatt
     4681 gccgcaaagc acggtgcggg actgacattg acatcaccgc tgccaggtga aagcggcggc
     4741 ttcgaggcgc gcctgcggtt aggcgccgct acagcaccag cgcacgcatc cagtccggca
     4801 gcggcaccgc cttttgcgcc ggaaagtcca cccagatgac cgtggccccg ccggcggcgg
     4861 ccagcacgcc gggctgatcc aggcgctcga tggtggtcca ggtctcgaac gtggtgcgcg
     4921 ccgggtcgct cacatagagc ttgagcagca gccgggccgg gtattcgagc tggcggtaga
     4981 agttgcagaa tgcattcacg atcaccggcc cctggccctt ggggctgggc tcgcaaccca
     5041 cggcccgcat ccactcgacg cgcacgctct ccatatagcg aaagtacacc gcgttgttca
     5101 catggcccat ggcgtccatg tcgccccagc gcacatccag ctccatgctg tgggccagct
     5161 tcttatgttc agcaatctcg attttcatgg tggtcttggt aagtcgttca aacagggcaa
     5221 gctgggcttg ccacggttcc gtgttggcgt gaatacaatc atcgccaaca cggccgcagc
     5281 cgcatggctg cttgggccgg cccattttcc aacgatccaa gcaatagaga cacccatgac
     5341 gaacgaagaa atcctcagca cctacggccc acgcgaagcc atggaatacg acgtggtcat
     5401 cgtcggcggc ggccccggcg gtctggccac ggcgattcgc ctgaagcagc tggcggcaga
     5461 aaaaggccag gaggtatcgg tggtggtgct ggaaaagggg tctgaacccg gcgcgcacat
     5521 cctctcgggc gccatcatgg atccgaaggc aatcaccgag ctgatccccg actggaaggc
     5581 caagggcgcg cccttgaacc agcccgtcac cgacgacgcc atggtgttcc tgggcgagtc
     5641 ctcgtcgttt cgcacgccca acttcctgct gcccggctgc tttcagaacc acggcaacta
     5701 catcgtcagc ctgggcaacg tggtgcgctg gatggccgag caggcggaag gcctgggcgt
     5761 ggagatcttc cccggctttg ccgcggccga ggtgctgtac aacgacgacg gctcggtcaa
     5821 gggcgtggcc accggcaaca tgggcatcag caaggagggc gagcccacgg gcgacttcca
     5881 gctgggcatg gagctgcatg ccaaatacac catctttgcc gagggcgcac gcggccatct
     5941 gggcaagcag atcatcgcca agtaccagct ggacgccgag agcgacccgc aaacgtacgg
     6001 cataggcatc aaggagctgt gggagatcga ccccaagcgc caccagcccg gcttcgtgct
     6061 gcacaccgcc ggctggccca tggacaacaa cacctacggc ggcgccttcc tctaccacct
     6121 ggaggacaac aaggtcacgc tgggcttcat caccggcctg gactacagca acccctacct
     6181 gagtccgttc gaggagatgc agcgctggaa gctgcacccc aacatccgct ggtacctgga
     6241 gggcgacgag gccaagggca tcaagccggc caagcgcctg ggctatggcg cgcgcgccat
     6301 cacggcgggc ggcctgctga gcctgcccaa gttcgtcttc cctggcggcg ccctggtggg
     6361 ttgcgacgcg ggctttttga acgtcagccg catcaagggc agccacgccg ccatcaagac
     6421 cggcatgctg gccgccgagg ccgcgttcga tgccattggc gccggtcgcc agcatgatga
     6481 gctgaccgcc taccccaagg ccttcgaggc cagctggctc tacaccgagc tgaacaagtc
     6541 gcgcaacttc aaggcctggt tcaagaaggg cctggtgatg gcctcggcaa tgaacggtat
     6601 cgagcagttc gcgctgcgcg gtcacattcc ctggacgctg cgccgcgata cgccggatca
     6661 cgcctacctg aagcccgccg ccgagtgcaa gcccatcgtc taccccaagc ccgatggcaa
     6721 gctgaccttc gaccgcctgt ccagcgtctt catcagcaac accaatcaca gcgagaacca
     6781 gcccgcgcac ctgacgctca aggacgccag cgtgccggtg aacgtgaacc tggccaagta
     6841 cgccggcccc gagagccgct actgccccgc cggcgtgtac gagttcgtgc ccgacgaggc
     6901 ccagggcggc aacgcccagc ggctgcagat caacgcgcag aactgcgtgc actgcaagac
     6961 ctgcgacatc aaggacccta cgcaaaacat cgtctgggtg acacccgaag gcggcggcgg
     7021 cccgaactat tcgggcatgt aagcccagcc ctggcagcaa tgattttgat agcttgctgc
     7081 gcttgtatat caagcgctgc aggccaattc cattaaaacc gttaaagtgt cgccggtacg
     7141 gggaacaacc gacgcgacca gaccgcgcaa acgctagact cgcgtgccag ccgcatgacc
     7201 ttcctgcatc ggcctggaat aacaagcaca ggaaacaccg cacgttccac tggagattga
     7261 accgaaatga agagcttgtc ttgggccccc ctgggcgcat tcgccctggc catggccacc
     7321 cttgcccccg ttgcacatgc gcagcaaaag tccgtggccg tgacggccat cgtggagcat
     7381 cccgcccttg actcggtgcg cgacggcgtg caggccgccc tgaaggccgc aggctacgag
     7441 tcgggcaaga acctcaagtg gcaataccag agcgcccagg gcaacaccgg cacggcggcg
     7501 cagatcgcgc gcaagttcgt cggcgacaac cccgacgcca tcgtcgccat cgccacaccg
     7561 tcggcccagg ccgtggtggc cgccaccaag accgtgcccg tggtgttctc cgccgtcacc
     7621 gatccggtgg ccgccaagct ggtgccgagc tgggagccga gcaagaccaa cgtgagcggc
     7681 gtgtccgacc tgctggcgct ggacaaacag atggatctgg tcaagcaggt ggtgcccaat
     7741 gccaagcgcg tgggcatggt ctacaacccc ggcgaggcga actcggtggt cgtggtcaag
     7801 gagctgcaaa agctgctgcc cacgctgggc atgaccctgg tcgaggccgc ggccccgcgc
     7861 tcggtggacg tgagcagcgc cgcacgcagc ctgatcggca aggtggacgt gatctacacc
     7921 aacaccgaca acaacgtggt ctcggcctac gagtcgctgg tcaaggtcgg ccaggacgcc
     7981 aagatcccgc tcatcgcctc ggacaccgac agcgtcaagc gcggcgccat tgccgcgctg
     8041 ggcatcaact accgtgacct gggcgagcag actgggcgca tggtggtgcg catcctgaag
     8101 ggcgaaaagc ccggcgacat caagcccgag accagcagca agctggagct gtttgtgaac
     8161 cccggcgccg ccgagaagca gggcgtgaag ctctccgagc agctggtcaa gtcggccgcg
     8221 caggtcgttc agtaacaata cggccccttg gccttgtgcg caggagcctc cggggctcct
     8281 gctttgtttt tgcccccagc ttccaagccc cgccccatgt ctcttttttc cttgctcggc
     8341 gccgttgaaa tcggcctgat cttcagcctg gtggcgctgg gggtctatat ctcgttccgc
     8401 ctgctgcgct ttcccgacct gacggtggac ggcagctttc cgctgggcgg cgcggtctgt
     8461 gccatcctga tttccaccgg caccaacccc tggctggcaa cgctggccgc cacggcggcc
     8521 ggcgcactgg cgggtttggt gaccggctgg ctcaacgtca agctcaagat catggatctg
     8581 ctggccagca tcctgatgat gattgcgctg tactcggtga atctgcgcat catgggcggg
     8641 ccgaacgtgc cactgatcaa cgacaccacc ctgttcaccc tgctgcagcc cgagagcatc
     8701 cccgactacg tggcgcgccc catggtgctg ttcgtggtgg tggtgctggc caagctggcg
     8761 ctggactggt tttttgccac cgagcgcggc ctggccatcc gcgccaccgg ctccaacgcg
     8821 cgcatggcac gcgcccaggg cgtgaacacc ggggccatgg tgctgctggg catggcgatt
     8881 tccaacgccc tggtgggcct ggccggcgcg ctgtttgcgc agacccaggg cggctcggat
     8941 atctcgatgg gcattggcac catcgtcatc ggcctggcgg ccgtgatcgt gggcgagtcc
     9001 atcctgccgt cgcggcgcat cgtctatgcc acgctggccg tgatcgtggg cgccatcgtc
     9061 taccgcttct tcatcgccgc ggcgctcaac agcgacttca tcggcctgaa ggcgcaggac
     9121 ctgaacctgg tcaccgccgc cctcgttacc gtggccttgg tgattcccca gctcaagcgc
     9181 aagctcagca gccgcaagtg atgcgccccc aggagaaacc acccatgctg agcgcacaag
     9241 accttttcat caccttcaac cccggcacgc cgatcgagac gcgcgcgctg cgcggcatgt
     9301 cgctggagat cccggccggc cagttcgtca ccgtcatcgg ctccaacggc gcgggtaaat
     9361 ccaccttttt gaacgccatc tcgggcgacc tgggcgtgga caccggccgc atcgccatcg
     9421 acaacctgga cgtgacgcgc cgccccgtgt gggagcgcgc cgagcgcgtg gcgcgcgtgt
     9481 tccaggaccc gatggccggc acctgcgaag acctgaccat cgaggaaaac atggccctgg
     9541 cgcagcagcg cggcacgcgc cgcgggctgc gcggcgccgt gaaacaggcc gagcgcgcca
     9601 tgtaccgcga gcgcctggcc acgctgggac tggggctgga aaaccgcctc accgaccgca
     9661 tcggcctgct ctcgggcggc cagcgccagg ccgtgagcct gctgatggcg gcgctgcagc
     9721 catcgcgcat cctgctgctg gacgagcaca ccgccgcgct cgacccgcgc acggccgact
     9781 tcgtgctgca gctcacggcg cgcatcgtga ccgagaacaa gctcaccacc atgatggtca
     9841 cgcacagcat gcgccaggcg ctggacgtgg gccagcgcac cgtgatgctg caccagggcc
     9901 aggtggtgct ggacgtgtcg ggcgaggagc gcgcacgcct ggacgtgccc gacctgctgc
     9961 agatgttcga aaaggtacgc ggcgaaaagc tggccgacga cgccctgctg ctgagctgat
    10021 ctccctcttg gcgggccgac accatcggcc ctcttcccct cttcatttcc cagtccacac
    10081 aggacaccaa ggcataggcg cgccgcacaa cggcggcgcc gcagccatcc cttcgaggac
    10141 tcaccatgag tgccagcgac tccacctccg gcaaccattt gcaccgcgcc ctaaaggcgc
    10201 gccacctgtc catgatcgcc atcggcggct ccatcggcac gggcctgttc gtcgcctccg
    10261 gcgccaccat ctcgcaggcc ggccctggcg gcgccgtgct ggcctacatg gtcgtgggtc
    10321 tgatggtcta ctttttgatg accagcctgg gcgagatggc ggcctatctg cccacctcgg
    10381 gctcgttcgc cacctacggc gcgcgctacg tcgatgaggg ctttggcttc gccctgggct
    10441 ggaactattg gtacaactgg gccgtcacca tcgcggtcga tctggtggcc gcgcaactgg
    10501 tcatggccta ctggttcccg ggtgtggatg gggtgctgtg gagcgcggtc ttcctggcca
    10561 tcatgttcgg cctgaatgcc atttcggtgc gcggctttgg cgaggccgag tactggtttg
    10621 ccgccatcaa ggtggccacg gtgatcgtct tcataggcat cggggtgctg atgatcctgg
    10681 gcatcatgcg cggcggcgca cccgaaggtc tgtggggcaa tctggcgctg tggacgcagg
    10741 acgatgcccc ctttgccggc ggctttgccg ccctgatcgg cgtggccatg atcgtcggct
    10801 tttcgttcca gggcaccgag ctgatcggca tcgctgccgg cgagtcggaa gacccggcga
    10861 ggaacatccc gcgcgccgtg cgccaggtgt tctggcgcat cctgctgttc tacgtgttcg
    10921 ccatcctggt gatcagcctg ctcattccct acaccgaccc acagctgctg aagaacgatg
    10981 tcggtgacat cgcggtgagc ccgttcaccc tggtgttcca gcgcgccggc ctgctgtcgg
    11041 cagcggcggt gatgaacgcg gtggtgctga cctcggtgct gtcggcgggc aattcgggca
    11101 tgtatgcctc cacccgcatg ctctacaccc tggcctgcca gcgcatggcg ccgcgcattt
    11161 ttgcccgcgt cagcgccaac ggcgtgccgg tgatggcgct gatcgccacc acggtggtgg
    11221 cggcgctgtg ctttctgacg aatatcttca gcccgcaggc ggtctacatc tggctgctca
    11281 acctctcggg catgaccggt ttcattgcct ggctgggcat tgccatcagc cattaccgct
    11341 tccgcaaggg ctatgtggca cagggccttc aactctccgc cctgccctac cgcaccacat
    11401 cgtttccatt cgggcccatc tttgccttcg tgctgtgcat gaccatcacg ctggggcaga
    11461 actaccaggc cttcctgcaa gagcgcatcg actggacggg tgtcgtggcc acctacatcg
    11521 gtttgccgct gttcctggtg atctggttcg gctatcggtt ggtgaagggc tcgcgcatcg
    11581 tgccctacgc caaaatgcag gtgaagcccg aggcgtattg acccagcgcc aaagacaggc
    11641 cggcctgccg ctacacaccc agcggcaggt cggccttttt cagcgtgcgc agcacaaagc
    11701 tcgaccggct gtggcgtatg cccttgatct tgtagagctt ctcgcgcaag aggcgctcgt
    11761 agtcgcgcgt gtccttgacc accacgcgca gcagatagtc atagtcaccc gaaaccaggt
    11821 gcgcctccac cacctcggga atgctggcca gcacctcgcc aaacttctcc agcgtgttgt
    11881 cgctatggct gtccagcgtc acctgcacca gcacggtgtc gcccagatcc agcgcctggg
    11941 cgttcaggcg cacggtatag ccctcgatca cgcccgactc ctccatcttc ttgatgcgcc
    12001 cccagcaggg cgacgggctc aggccgacgg cggcgccgat gtcctgcagg ctggcgcgcg
    12061 cattgccctg caacacggag agtatttttc tatcaagcct gtccatacgc tgcattgttc
    12121 actattttgc gtatatgcag aaaattattc tgcaaattcg gaaattttga agaaattaag
    12181 caaatctatc cagaccgtcc caagcatact ctgtacatca aggcgctctt accggggcgc
    12241 acaacattga caggccccca cccggttacc gccgggcggg gtcttggcaa gctgccaggc
    12301 cggcgcaaaa aaactcatcc cggcggtgca aaacgctcaa tcactctttg gcagcctggc
    12361 gcaagcgatg cgcgtaaaac ctctttgtta ctacctctta cccggaaaag acacgggctc
    12421 tggacagacc agacaaccca accgaaggag agagccagat gacagagcag accgtagaga
    12481 caggccgccg caagcttctc aagggcgcag ccgttggcgc aggcgccctc tccgtgccaa
    12541 tgatcagcac ggcgcagacg accaccatgc gctttcaaag cacctggccg gccaaggaca
    12601 tctttcacga atttgccgtg gacttcgcca agaaggtgaa cgacatggcc ggcaatcggc
    12661 tgaagattga agtgctgccc gcaggcgccg tggtgcccgc cttccagctg ctggatgcag
    12721 tcgccaaagg cacgctggac ggtggccacg gcgtggtggc ctattggtat ggcaaaaccc
    12781 aggcggtggc gctgtggggc tctggcccgg catttggcat ggatgccaac atggtactgg
    12841 cctggcacaa ctacggcggg ggcaaggagc tgcttgaaga aatctacaag agcctgaacc
    12901 tcgatgtggt ctacaagctc tacggcccca tgcccacgca gcccctgggc tggttcaaga
    12961 aacccgtgtc caaggtggaa gacctcaagg gtttgaagtt ccgcaccgtc ggcctggcgg
    13021 tggatgtgtt caatgagctc ggcgtggccg tcaatccgct gccaggtggc gagatcgtgc
    13081 cggcgctcga tcgtggcctg ctcgacgcgg ccgagttcaa caacgcctcc agcgaccgcg
    13141 tgctgggctt tcccgacgtg gccaagaact gcatgctgca gagctttcac cagtcgagtg
    13201 agcagttcga ggtgctgttc aacaagacca ggtacgacgc gctgccggca gagctcaagt
    13261 ccatcatcga ctacgccacg caggccgcca gcgccgacat gagctggaag gctgccgacc
    13321 gctacgccaa ggactatgcc gaaatgcaag ccaagcaggg cgtgaagttc tacaagacgc
    13381 ccgacgccat cctgcgcgcc cagttggccg cctgggacaa ggtcacggcc aagaaggcgg
    13441 aagagaaccc gatgttcaag aaggtgctgg agtcgatgcg tgcatttgcc cagcgcagcg
    13501 gacgctggca gaacgacacc accatcgact accggatggc ctggaatcac tacttcgcac
    13561 ccaagaagaa ggggtaagtc cgaccgcgac aagaaggcct tgaatcaggc cttctttttt
    13621 gcctgccacg gaggggcaag cccccccgca aggcacgcac tcccctttcg cctgcgagcc
    13681 cctgcggctc tgtcaccatg caaaaactgt tgcttcggat cgacgccgtg tcgaccttca
    13741 ttggcaaggc agccgcctgg ctttgcgtac cactgacctt gctgatctgc tgggaggtct
    13801 tttcacgcta cgcactcaac aagccccacg cctgggtgct ggacgcgcaa atcatgctct
    13861 acggcaccat gttcatgctt gccggcgcct acaccctggc caagaacggc catgtacgcg
    13921 gcgacgtgct gtacggcttt ttcagccccc gcgtgcaggc cgggctggat ctggccctat
    13981 acggcctgtt cttcctgccg ggtgtgctcg ccatgacctg ggccggctgg aactacgcgc
    14041 aggagtcgct ggcgattcgc gagcagacct tctcggccga gcccttgccg ctgtatccgt
    14101 tcaagttcgt gattccgctg gccggcgccg cgctgctgct gcaggggctg gccgaaattg
    14161 cgcgctgtgt gatctgcctg cgccagggcg catggccaag gcgcgaagac gacgtggagg
    14221 aagtggacgt cgaaaaactc aagcgaatgg tgcatatcga gggcaccatg tacgaagcca
    14281 gccagcccac gtccacaccc ggccagcagg ggggcaagcc atgaagctgc gcaaggaact
    14341 gtggttcggc ctgtccatca tggccctggt ggtgggcggc gcggcctggg ggctgctgag
    14401 cacacccacc atcaccaacg gccacctggg cctgctgatg ctgtcgctgg tggtggtggc
    14461 catcatgctg ggctttccca ccgccttcac cctcatgggt atgggcatga tcttcacctg
    14521 gctggcgtac gaccgggaca tcacccgctc gctcgacctg atggtgcagg ccgcctacaa
    14581 gagcatggtc aacgacgtgc tgattgccat cccgctgttc gtgttcatgg gctacctggt
    14641 tgagcgcgcc aacctgatcg aaaagctgtt caagagcctg cacctggcga tggcgcgcat
    14701 cccgggctcg ctggccgtgg ccacgctggt cacctgcgcc atctttgcca ccgccaccgg
    14761 catcgtcggt gccgtggtca cgctgatggg cctgctggcg ctgccggcca tgctacgcgc
    14821 cggctacagc atcccgcttt cggccggcgc catcacggcc ggcggctgcc tgggcatcct
    14881 gattccgcct tcggtcctgc tcatcctgta tggcgcgacc gccggggtgt cggtggtgca
    14941 gctgtacgcc ggcgccttct tccctggcat cgtgctggcg ggcctgtatg tggtctacgt
    15001 ggtcatagtg gccaagatca agccggcctg ggcgccacca ctgtccgatg gcgaacgcca
    15061 ggtcgatctg ccggcgctga ccgccgccct gcccgcgcag accggccagc atgccgtggt
    15121 gcgactgctg gacgcgctca agggccggcg caacgcccag gtgccactgg cccatgtgct
    15181 gcgccagctg gccctggtgg tcttgcccgg gctgctgttt ctggtgctgg tggtgagcag
    15241 ctggaacgcc gtcaccacgg tcaaggtgga ggagacgttc gacatccagc ccataggcct
    15301 gggcgaaagc agctcccctg cctccggcct gcaagagccg cccggcgacg gaggtttgca
    15361 agagcccccg gcagagggca gcctccagga gccccccgca gagcaagccg aagagaacca
    15421 gccggatacc gcgctggccg agcccccggg cgccgagccc gaagttcagg cacagggcca
    15481 ggatggcgcg gccgtggccg aaccgccggc cgctgcagcg gccgaggccc cagcccaggc
    15541 cgagacgcag ccggccccca cctggtggtg ggtgttgttc tcgctgctgg gggcggtcac
    15601 ggccctgttc tacctgttcc tgagctttgc ccggcttgaa atcttcaaga tgctgctgag
    15661 ctccttcttc ccgctgatga tgctcatcct ggcggtgctg ggctccatcg tcttcggcct
    15721 ggccacgccc accgaggcgg cggccatggg ggcgctcggc ggctttctgc tggcggcggc
    15781 ctaccggcag ctgaccatgg acgtgctgcg cgagtcggtc tacctgacgg ccaagacctc
    15841 ggccatggtg tgctggctgt tcgtgggctc ggccatcttt tcagccgcct ttgcgctgct
    15901 gggcggacag gaagtgatcg agaaatgggt gctgtccatg aacctcacca aggtcgagtt
    15961 catggtgctg tcccagatcg tcatcttcct gctcggctgg ccgctggagt ggacggagat
    16021 catcgtgatc ttcatgccca tcttcattcc actgctgagc cacttcgggg tcgatccgct
    16081 gttcttcggc ctgctggtgg cgctgaacct gcagaccgcc tttctgtcac cacccgtggc
    16141 catgtcggcg ttctatctga agggcgtggc gccgccgtat gtgacgctga accagatctt
    16201 cctgggcatg ctgcccttca tgggcataca gatcctggcc atcgtgctgc tgtatgcctg
    16261 gccgtccata ggcctgtggc tgccgcagct gctctacaag taaggccagg ggccgtcgcc
    16321 tgtgatcaga ccatctgata ccaggcgcag ccctccttcc ccattctcac cctggcctcg
    16381 ccacgcgcca ccaggtagtt gagatgggcc acgctttcgc ccgtggccag gctcatctgc
    16441 atgttgtccg cggtgaccgg gcgggaaaac agcgcgccat agacatcgac cacgcgctgc
    16501 ggttgccgca aggcctctcg cagcctgtcg agcgcccgat gctggctgtc gcgcagcgcg
    16561 gcaatgcgcc gatgcaggcc ccggaagggc tggttgtgcg atggcagcac cagcacgtcg
    16621 cccggcacgc tgcgctcgag cttttccagc gactccagcc agtcggccaa cgggttggca
    16681 tcgggctcgg ccggaaacac cgagacgttg gacgaaatgc cgggcagcac ctggtcgccc
    16741 gagatcaaca gcttcagctc ggggcagtac aggcagacat gctcgggcga atggcccgcg
    16801 cccaccacca cgcgccagcg ctgcgcgcct atggcgatct cctcgccatc gcgcaggcgc
    16861 cggtaactgt cgggcagcgc atagatcagc ttgccaaagc cgccaaagcg ggtgcgatag
    16921 gtctcgatcg cctcctcgcc ccagccggca cgccggcaga acgcgatggc gtcatccggc
    16981 gcttcgcgcc cggtatcgac gctcaatgcg cggcagctca ggtactccag gcggctgatc
    17041 cacagccggc aggcaaactt gcgcgtcagc cagccggcca tgccgatatg gtcggggtgc
    17101 atgtgggtga cgaaaacacg cgtcaccggc ccatcgagca cccccgcaaa cagctggcgc
    17161 caggcacccg ccgtgtcggt ggaccagatg ccggtatcga ccagcgccca gccatcgcca
    17221 tcacgcaaag cccacaggtt gatgtggtcc agcgcaaacg gcatgggcat gcgcagccac
    17281 catacgcccg gggccagttc tttggcgtcg cctaccgcgg gcgcaacgcc gcatgggtag
    17341 accaggccgt tcttgtccgt caccacttct tcagccattg atgccactac cgaccttcat
    17401 ttttgttacc aactggcagc ttatcggaaa acaccaggac gcattgccca gcctgtcgcg
    17461 caaggtagaa ttgccggctg tcaggagaga gcccccccgc ggggccgccg aaggcgcagg
    17521 cagcagccaa aacgctcagg caaaaggact gacgacacgc ccgcgattgc tgcgggcgtt
    17581 tccttgctgg agagaggcgc cccaaccggt ttcgggcgtc caccgaagga gcaaaccctg
    17641 ccatccgggg tgaatctctc aggtaaagag gacagcaggg caaacaccat ggtgcgttgc
    17701 gccatggccc gcatttgccc tggagacatg ccttgtccga caccgaactg ctgaccaccc
    17761 ccctgaacgc cctgcacatc gagctgggcg cccgcatggt gccctttgcc ggctactcca
    17821 tgcccgtgca gtacaagagc gggctgatgg ccgaacacca gcacacgcgc caggccgccg
    17881 gcctgttcga cgtctcgcac atgggccagc tcaagcttat cggagccgac gcggcggcgg
    17941 cgctggagtc tctgatgccg gtggacgtgc agggcctggc cgaaggcaag cagcgctacg
    18001 gcctgctgct caacgacgag ggcggcgtga tcgacgacct gatgttcttc aaccagggca
    18061 acgacacctg gttcctgatc gtcaacggtg cctgcaagct caccgacatc gcgcatatcg
    18121 agacgcatat cggcaaccgc tgccagatcg tcaccctgcc cacccagggc ctgctggcgc
    18181 tgcaggggcc gcaggcggtc acggccctct cgcgcctggt gccgggcgtg gaaaagctgg
    18241 tcttcatgag cggcgccagc ttcgcctgga atggcgccga gctgttcatc acgcgcagcg
    18301 gctacaccgg cgaggacggc tttgaaatct ccgtgcccgg cgagcacacc gagaccctgg
    18361 cacgcgccct gctggcccag cccgaggtga agccgattgg cctgggcgcg cgcaattcgc
    18421 tgcgcctgga ggccggcctg tgcctgtacg gcaacgacat cgacagcacc accaccccgc
    18481 ccgaggcggc gctgaactgg gccatccaga aggtgcgccg cagcggcggc gagcgcgctg
    18541 gcggctttcc gggcgcggcg aaggtgctgg cgcagatcga caacccggcc agcctgtcga
    18601 agaagcgcgt gggcctgatc gccaaggagc gggtgccggt acgcgagccg gcggtgctgg
    18661 agaacctgga cggccagcat gtcggccatg tcaccagcgg cctgctctcg cccacgctga
    18721 accagcccat cgccctggcc tacgtgcagc ccgagtacgc ccagcccggc accgaggtgt
    18781 ttgccatggt gcgcggcaag cccgtggcca tggtggtctc gcccacgccc ttcctgcccc
    18841 cgcgctacca ccgcggctga ttttttcggt ttcgctacac cggcagcgcg gctacagtgc
    18901 gcgctgcccc ttctcacaac ttttttatcg gagcatcgcc atgagcatcc agttttccaa
    18961 agaccacgag tgggtcaaca gcgccgacgc caacgctgcc gtggtcggca tcaccgtaca
    19021 tgcgcaggac gcgctgggcg acgtggtctt cgtcgacctg cccgccgtgg gcgccacctt
    19081 tgcccagggc gacgtggccg gcgtggtcga gtcggtgaag gccgcggccg acgtctacat
    19141 gccggtgagc ggcgaggtcg tcgaggtgaa cgaggccctg cgtgacgatc cttcgctggc
    19201 caactccgac cccatgaacg ccggctggtt cttcaaggtc aagctctctg cccccgccga
    19261 gctggacgcg ctgatggacg aagcggccta caacgccttt gccgccagcg cgtgatgagc
    19321 gttgagcgtt gagcgttgag cgtcccctca gacgctcaac gccaaatgca tcacccaaaa
    19381 atatgagctg cccgcgctga tccagcaagg gctaccagcc aatctgacct gaacccaccc
    19441 catgcctgcc ctgcccctct ccgcgctgga aaaccacgcc gaattcctgc ctcgtcatat
    19501 cggtatcgat agcgctgacg aggcgctgat gctgtccgtg attggcgagg cctcgcgcca
    19561 gtcgctggtg gcctccatcg tgccgcgctc catcgcgcgc gccagcagca tggatctacc
    19621 cgcgccctgc accgaggccg aggcactggc cgagttgaaa acaatagccg ccaagaacca
    19681 gatcaagaaa agcttcatcg gccagggcta ctacggcacg catacgcccg gtgtcatcct
    19741 gcgcaacatt ctggaaaacc ccgcctggta caccgcctac acgccctacc aggccgagat
    19801 cagccagggc cgcatggagg cgctggtcaa cttccaaacc atggtcaccg acctgacggg
    19861 catggacatc gccaacgcgt cgatgctgga cgaagccacg gccgccgccg aggccatgac
    19921 gctggccaag cgttccgtca aggcccgggg caacagcatc atcgtgagcg gcgactgcca
    19981 cccacagacc atcgaagtca ttcaaacccg cgccgcgccg ctgggtttca ccgtcaaact
    20041 cgtgcagtcc gaggccgagt ggaacgccgc catcgccagc gacgactact ttgccgccct
    20101 gcagcaatac cccagctcca gcggctggct ggccgactgg caggcatcca gcgacgccat
    20161 ccacgccaag ggcgcggcct tcatcctggc ggccgacctg ctggcactga ccctgctcaa
    20221 agctccgggt gagatgggcg ccgacatcgt catcggcacc acccagcgct ttggcatgcc
    20281 catgggcgcg ggcggcccgc acgccggctt catggcctgc aaggacgcct tcaagcgctc
    20341 catgcccggg cgcctggtgg gcgtgagtgt ggacgtgcac ggccagcccg cctaccgcct
    20401 ggcattgcaa acgcgcgagc agcacatccg ccgcgaaaag gccaccagca acatctgcac
    20461 cgcgcaggtg ctgcccgccg tgattgccag catgtacgcc gtctaccacg gcgctgcagg
    20521 gttgacccgc atcgcccagc gcgtggcacg cctcaccgcc atcctggctg cgggcctgaa
    20581 acaactgggc ctgcctgccg aacacgccag cgcctttgac acgctggccg ttgacctggg
    20641 cagcagcgca gccgccgatg ccaccctggc ccgcgccctg gcccttggtg ccaacctgcg
    20701 ccgcctggac gcacagcgcc tggccgtgtc gctggacgag accaccaccc gcgccgacct
    20761 ggccctgctg tggagcgcct tcgcacccaa tgctgcccag cccagcgtcg aggcgctgga
    20821 aggcgccgcc agcctgatcc cggccgccct ggtgcgcgcg agcagcttcc tcacgcaccc
    20881 ggtgttcaac acgcaccact ccgagacggc catgctgcgc tacatccgcc agctgtccga
    20941 caaggatctg gcgctagacc gcaccatgat cccgctgggc agttgcacca tgaagctcaa
    21001 cgccaccagc gagatgatcc ccatcacctg gcccgagttt gcgcatatcc accccttcgc
    21061 cccggccgag caactggccg gctacgccga gctcgacgca ctgttgcagc gctggctctg
    21121 cgccgccacc ggctacgccg gcgtgagcct gcagcccaac gcgggttcac aaggcgaata
    21181 cgcgggcctg ttggccatca aggcctacca cgaggcccag ggccacgcac accgcaacat
    21241 ctgcctgatc cccagcagcg cgcacggcac caacccggcc agcgcgcaaa tggtgggcat
    21301 gcaggtggtg gtgaccaagt gcgacgagaa cggcaacgtg gacatggccg acctgcaagc
    21361 caagtgcgag cagcacagcc agaatctggc ctgcgtgatg atcacctacc ccagcacgca
    21421 cggcgtgttc gagacccagg tcaaggagct gtgccagatc gtgcacagcc acggcggccg
    21481 cgtgtacgtg gacggcgcca acatgaacgc cctggtcggc gtggccgcgc cgggcgagtt
    21541 tggcggcgac gtgagccacc tgaacttgca caagaccttc tgcatcccgc acggcggcgg
    21601 cggcccgggc gtcggcccgg tctgcgtggt ggaagacctg gtgccctacc tgccgggcca
    21661 cggcacgtct ggaaacgcta gcaaagtagg agctgtaagc gcagcaccat tgggcaacgc
    21721 agccgttttg cccatcagct ggatgtacat ccgcatgatg ggcgccgagg gcctgacggc
    21781 cgccaccgag gtggcgattc tgagcgccaa ctacatcagc aagcgcctgg ccgagcacta
    21841 ccccacgctg tacgccagcg ccaacggcca tgtggcgcac gagtgcattc tggacctgcg
    21901 ccagctcaag gagtccagcg gcgtgatggc cgaggacgtg gccaagcgtt tgatcgacta
    21961 cggctttcac gcgcccactt tgagcttccc tgtcgccaac acgctgatgg tcgaacccac
    22021 cgagagcgag cacctggccg agctggaccg ttttgtggac gccatgatcg ccattcggga
    22081 agaaatccgc caggtggaaa ccggcgcctg gccgcaggac gacaacccgc tcaagaacgc
    22141 gccacacacc gccgagagcc tgctcgccgg cgactggagc cacccctaca cgcgcgaggc
    22201 ggctgccttc ccggtcaagt ccttgcgcgg cgccaagtac tgggcgcccg tgggccgcgt
    22261 ggacaacgtc tatggcgacc gcaacctgtt ctgcagctgc gtgccgatga gtgagctggc
    22321 gtaaaaccgc cgcgcacgga gaaatgtgga tggccagcga aaatctaaaa cccgaaaaac
    22381 tggccgtcct cattgacgcc gacaacacga catccagctg cgcacagggc ttgctggagg
    22441 aaatcgccaa gtacggcatc gccagcgtca aacgcattta cggcgactgg agcagccccc
    22501 tgctcagcgg ctggcgcagc attcttctca agcacgcgct ggtacccatc cagcaattcg
    22561 cctacacgaa gggcaaagat gccaccgaca tggggctgat catcgacgcg atggatctgc
    22621 tctacagcgg ccacttcgac ggcttctgcc tggtctccag cgacagcgat ttcacgcctc
    22681 tggcatcacg catccgagct tcgggccgaa tggtctatgg ctttggcagg gagaaaacac
    22741 ctgaggcatt caggcaagcc tgcgaccggt ttttctacat tgaaaacctc ggtgaagcag
    22801 gaaaaggcaa ggatgacatc gttgccgtgc cgaatgcagt tatggccgcc tcgcctgaca
    22861 ttgccaaacc tgccgccaag ccaaggcaaa tggatggaac caccaaaaac ctgttgtaca
    22921 aatcgatcaa ggacgccacc gacgaaacca ccggttgggc cttcgttgga aaaatcggca
    22981 acgtcatcag tgaaacgcgg ccagacttcg actctcgcac ctatggttac gccaagctct
    23041 ccggaatgct gcgcgagcta cgaggcctgc aatttcgcac cgacgaagcc aaccgcatgt
    23101 attgtcggaa aatacctttt ggcgatctca tcaaattgct tgatgaagct ttcaacaaat
    23161 tcaagaacgc caagggctgg gccagccttg acgtgactgg caagtacgtg aagccgcgct
    23221 ggaactggga agagtatggg tttgaaagct tcactgatct tctcagcaag gtagatcatg
    23281 tggaaatcgc caacgacagc atgcgcatgc aggtatccat tgcgccgtag cgcccctgtt
    23341 ctgcagctgc gttccattgt ctgatctcga ctaaagtcaa tccgtaagca ccctcaaagg
    23401 ccctaccatc gggcctttgt ttttggaagg cttccatgcg caccaccctg accctgctcg
    23461 ccgctctttt tctcactccg gtggccctgg cgcagtccac cccggtcacc acggccagcg
    23521 gcgtggtcta tgaatccctt catgacggca caggcatcag ccccaaggcc ggcgacacgg
    23581 tcaaggtcca ttaccgcggc atcgccggcg acggcaagga aatcgacagc tcctacaaac
    23641 gcggggagcc ggccgagttc ccggtcaacc gcgtcattcc ctgctggcgc gagggcctgc
    23701 agctcatgaa ggagggcggc aaggccaagc tctcctgccc gcccgcaacc acctatggca
    23761 tgcgcggctc cagcgccctg atcccctcca acaccacgat gcagttcgag atcgagctgc
    23821 tgtcggtgcg ctgacagccc atcgagccgc agccgccatg ggccagcccg acctgtcctt
    23881 tctgctgcgg ggcatggagc cgccgcagcc gccgggcgat gccacgtcac ccgcgggcat
    23941 gcccgagcgc cgcagcccgg aacgcgccag cggcgccgtg gcccagctga tcgccgagct
    24001 gcgcgcctcg caggccgagc tggagacgca gaacaaggtg ctgcgctaca gccaggcggc
    24061 ggcggaaagc gcctcggagc gcttcgaggc cctgtttgcc aatgtgccgc tgtcactcat
    24121 ggtgctggac gcgcacgaca tggtggtgca ggccaactcg atggcgcagc gctcgttcca
    24181 gccgcaggag aatgaccgcc cgctgatcgc cctcatgccc ttcgtcggcg ccgagcatgc
    24241 gccgcgcgtg caggccgcgt ttgcagcggc cacccaacag ggcacggccg aggccaccga
    24301 ggtagtgttc cacatcgacg acgagcggcg catcacgggc gacctgcata tcgcgcgcat
    24361 cgaatcgcac agcgacgatg gcacggtgct gacgcagttt ctctgcgccg tgatcgacca
    24421 gggcccgctg ctggccgagc gccaggcctt gcagcgcagc gccgcagagc tgcaggagcg
    24481 caacgcgcag ctgcatgccg gcgagcgccg gctggaggcg gtgatcaact ccgcgctgga
    24541 cgccatcatc tgcgtcgatc aaagcgagca catcaccgtc ttcaacccca ccgcagccat
    24601 gctcttccag tgcgccgcca gcgacgccct gggcagcccc ctgagccgct ttctgcccga
    24661 cgcccaacag gtgctggcct tcgcccaact caccacccag gcggcgctcg gcgagatgac
    24721 agccctcacg gcgggcggca acgagctggc agtggaggtg aatgtgtcgt ttgagcgcca
    24781 tgcagagggg gaaaccacca ccgtgttcgc gcgcgacctc acgggcaaga agcgcgagga
    24841 agcgcgccgc aatgcccttg aggcgcagct gcgcgagtcg cacaagatgc aggccgtggg
    24901 caccatggcc ggcggcatcg cgcacgactt caacaacatc ctgcatgcca tcctgggcaa
    24961 cgtggagctg gccaaggccg actgcacgcc aggctcggca ctgcacgaaa gcctgctgga
    25021 gatagacaag gccggccggc gcgcgcgcga cctggtgcgc cagatcctga ccttcagccg
    25081 caacgaggcg ccccggcgcg ccgccgtcgc gctggccgag gtggcgcatg acaccgagcg
    25141 cctgctgcgc gtcacgctgc cacccgagat cgagctgagc ctgcacctgc agcccgagct
    25201 gcccccggtg ctggccgatg ccacgcaggt cgagcaggcg ctcctgaacc tgtgcaccaa
    25261 cgccgtgcac gccattggca atcggcgcgg gcgcatccag gtcgatggcg tgctggtgca
    25321 gcccgaccag caccagcgcg aaaacctggg cctggccgcg ggcgactatg tggcgctgcg
    25381 cgtgcaggac gacgggcccg gcatggacga ggccacgctg ccgcgcatct tcgagccctt
    25441 cttcaccacc aagcccgtgg gccagggcac gggcctgggc ctggccgtgg tgcatggcgt
    25501 gatgcgcacc catggcggcg ccatcgacgt gcacagcgcg ccgggccggg gcagcagctt
    25561 cacgctgtac tttcccgtgg ccaccgcgtc accgcaggag ggcgccgcca cggccacacc
    25621 gcccgcacct gctcccgagc ccgcaccacc cgcgagccgg cgccagcgcc atgtgatgta
    25681 cgttgacgac gacgaggcgc tggtgtttct ggtgcggcgc ctgctgcgcc gccggggcta
    25741 cgaggtcagc ggcttcaccg acccgcacga ggccacggac gcattgcgcg ccgcgcccgg
    25801 gcgctacgac ctgctggtca ccgactacaa catgcccggc tactcgggcc tggacctggt
    25861 gcgcgcggcg cgcgccatca ggccggatct gccggtggcg ctggcctcgg gctatgtcac
    25921 ggccgagatc gagcaggcgg cgctggccga aggcgcgcgc gcgctgatcc acaagcccaa
    25981 cgatgtggcc gagctctgcg cgaccgtgga caggctggcg catgagcgat gaggcgctcg
    26041 ctgggcttgc ctgcctgcat gccgacgacg acctgctggt gctggacaag ccccccggcc
    26101 tgctgtgcgt gcccggccgc gggccggaca agcaggattg cctgagcacc cgcgcccagg
    26161 cgctttggag cgacgcactc atcgtgcacc gactggacca gcccacctcg ggcctggtgc
    26221 tgatggcgcg cggcctgccc atgcagcgcg cgctgagcag gatctttgcc gagcgccgcg
    26281 tgcacaagcg ctaccaggcc gtggtggccg gcctgccgca acccgatgac acggccgatg
    26341 acgaaggctg gagcgccatc gccctgccga ttgccgccga ctgggagcgc cggccgctgc
    26401 gtgtggtgga tgcacagagc ggccagccca gcctcacgcg ctggcgggtg ctggacgtcg
    26461 accgggcttt gggctgcacg cgcctggagc tcgaacccgt caccggccgc acgcaccagc
    26521 tgcgcgtgca tctggcggcg ctgggccacc cgatactcgg cgacatgctc tacgccaacg
    26581 ccgcgcaaca ggcaagcgcg ccgcgcctgc tgctgcacgc ctgcacgctg gaatttgccc
    26641 atcccctgca tgggcgccgg tgcagctaca tctcggcagc gccattttga gaatgcggcg
    26701 cgcctgaccg ttaccatgca gggatgacac aacagctctt catcctcacc ggcgcctcgc
    26761 gcggcatggg actggccatg gcccagcagc tgctgcaacc cggccacacc ctgatctgca
    26821 tggcgcgcaa cagcaattcc cagctgaccg ctgccgtgcc cgccggcgcc atgctggagc
    26881 aatggaccgt cgatctgtcc gagcccgcac ccgcggccca gcagctgcat gcctggctgg
    26941 ccgggcaaag cgccgggcgc tacgccagcg ccacgctgat caacaacgcc ggcgtgatcc
    27001 cgcgcattgc gccgctgtcg gcctgcgcca gcgcgcagga catggccgac caggcgcgcg
    27061 cgctgcgcgt cgggctggag gcgcccatgc tgctcacggc cgccttcctg ggcgccacgg
    27121 agggctgggg cgccccgccg cacgccgttg cacgcaaggt gctcaacatt tcctcgggcc
    27181 tgggccggcg cgccatggcc tcgcaggcgg cctactgcgc ggccaaggcc ggcatggacc
    27241 atttcacgcg ctgcctggcg ctggacgagg ccgccaaggc gcatggcgcc agcgtctgct
    27301 cgctggcgcc cggcgtcatc gacaccgaca tgcagatcca gctgcgcggc gccagcgcgg
    27361 ccgactttcc cgacgtgcag aactttgccg ccctcaaatc cggtggcatg ctgaccacac
    27421 ccgctgccgc ggccgcccgc gtgctggcct ggctggagcg cgccgacttc ggcagcaatc
    27481 cggtggccga cgtgcgcgac gcttaagaga taaattacta tattttttgt agctattgac
    27541 gcttaccccc tgggcgtaaa agcccttttc aacctgaaac cttccactac aggagagaac
    27601 catgacccgt gaagttgtcg ttgtcagcgg tgtgcgcacc gccatcggaa cctttggcgg
    27661 cgcgctgaag gacaccccca ccgtcgatct cggcgcgctg gtggtcaagg aggcgctggc
    27721 gcgcgccggc gtggagggca aggacgtggg ccatgtggtc ttcggccatg tggtgaacac
    27781 cgagccgcgc gacatgtacc tctcgcgcgt ggcggccatc aacggcggct gccagaaaga
    27841 gacgccggcc atgaacgtca accgcctgtg cggctcgggc ctgcaggcca tcgtcagcgc
    27901 cagccagggc atcctgctgg gcgactacga catcgccatc ggcggcggcg ccgagaacat
    27961 gagccgcgcc ccctatgcca gcctggccac gcgctttggc gcgcgcatgg gcgacagcaa
    28021 gatggtggac atgatggtcg gcgcgctgca cgaccccttc cacaccatcc acatgggcgt
    28081 gacggccgag aacgtcgccg ccgagcatgg catcacgcgc gagatgcagg acgcgctggc
    28141 gctggagagc caccgccgcg ccgaggtcgc ctgggccgag ggccgcttca aggaccagat
    28201 cgtcccggtg atgctcaaga gccgcaaggg cgaggtggcg tttgacaagg acgagcattt
    28261 ccgccccggc gccaagctgg aggacttcca gaagatgaag gccgtgttcc tgaaggagaa
    28321 cggcaccgtc accgccggca acgccagcgg catcaacgat gccgctgccg ccgtgctgct
    28381 gatggaggcc ggcgccgcca aggcgcgcgg cgccaagccg ctggcgcgcc tggtgggcta
    28441 cgcccacgcc ggcgtcgagc ccaagaccat gggcattggc ccggtgccgg ccacacagca
    28501 actgctgcaa aagctgggca tgaagatcga ccagatggac gtgatcgagg ccaacgaggc
    28561 cttcgccgcc caggcctgcg ccgtcaccaa gggcctgggc gccgacccgg ccaaggtcaa
    28621 ccccaatggc tcgggcatct cgctgggcca ccccatcggc gccacgggcg cgctgatcac
    28681 cgtcaaggcg ctgtacgagc tgcagcgcat ccagggccgc tacgccctgg tgaccatgtg
    28741 catcggcggc ggccagggca ttgccgcagt ctttgagcgc atgtgatgac ccgccccata
    28801 tagctacaac taaagtagct atcagcgccc gctggacaag cgccagcggg cgtttttatt
    28861 gaatatcacg aagtcgtgat accggcgcaa ccactggcac catcggcgcg cggacgccag
    28921 aacaccggca gcatatgcgc gctctgcagc aagaaggccg cgccccacag caacgcggcc
    28981 gcgcccatca tggccggcgc ctgcccggca taggcgccca cgcgcagcag cgcgctggcg
    29041 cccagcagca gggcgcccag cggcacccag gggcgagcgc ccttctccag cccgctgtgc
    29101 gtataaccgg caatgcagat cacgacgtag atgctgagcc cgagcacgcc cgtggtcagc
    29161 aggtgcaggc cggcgttggg gctgaaagcg cctgtcagca ccgccacgcc gatgagcgcg
    29221 tagccgcccg ccatcaacag gtacacggcg tacagcatca acggccagcg ccgcagcagc
    29281 ggccgcccca catgccagtc gttcagcaga ttcagcaggg cgcaggccgc cgccagcgcc
    29341 agccagccgc tgaccggccc acccgcctgc cagaactcgg ccagcgtgaa caggcctatg
    29401 gtcagcagcg ccagcttgcg cctgggcgga cgggccaggt agggcgccag atcctcgccc
    29461 tggcgcgcgg ccagctcgtc gatgctggcg ttgacgatgc tcatcgagat gcggctcatg
    29521 gccaccacca ccagggccat gaagacgccc accagcacat gcagccagcg cgccggcgag
    29581 atgccccgca gcgcgtcgaa gtacaaggcc gcgaccaccg cggccagcgc cagcagcgtc
    29641 cataagaacg acagatggcg cctgtccggg tcgcgccaca ggcgcggcgc cagcaaggcc
    29701 atcagcccgg ccagcaggcc caggtgggcc agcgccgaca gcgccagcgc cgggcccggc
    29761 caccagccgc tggaccagaa ggccagccgc cccagcagcc acagcagcac cagctggcgc
    29821 acgggcccgg ccgcgaatgg tggcgtgccg gtgaactcag gcaccgccgt gagcacgaag
    29881 ccggccagag ccgccagcac aaaacccagc agcagctcat gcgcatgcca gacaaagggg
    29941 ccgcccggca ccacgggcag cggccagccc agcagcaaaa aaccgcccca cagccccatc
    30001 agcaaccagg ccgacagcat ggccagcgca aagaacgggc gaaacgggca cagccacagg
    30061 gggtgggcga gcgaccaggg cgtggggcgc ggcgccattg ccattgccag cggcgccgtc
    30121 acagcgccac ctcgcaaccg tcgccggcga tgcgcgcctg gccgcccacg gccacgccgg
    30181 tgcctgggtc ggcgggggaa tatgcgtcgg cgggcgacac cccatgggcc agcggcggca
    30241 ggtcgaacgc cgcgcgcacc gccggct
//
LOCUS       DAOMTQ010000014        30005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_126_length_30005_cov_26.850217, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000014 DAOMTQ010000000
VERSION     DAOMTQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 30005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30005
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_30005_cov_26.850217"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..496
                     /locus_tag="PK518_02700"
     CDS             80..496
                     /locus_tag="PK518_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HRM47848.1"
                     /translation="MPAASALPACPCGRTDSRRRALPYAQCCGRYIDHFAQCPAPDAE
                     SLMRSRYTAFVREDAAYLLATWQAAHRPAQLDFEPGARWLGLQVRAHLPLDATHAEVE
                     FVARQRDATGRAHRLHERSRFLREQGRWYYVDGKLI"
     gene            complement(551..2056)
                     /locus_tag="PK518_02705"
     CDS             complement(551..2056)
                     /locus_tag="PK518_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM47849.1"
                     /translation="MPATALTRRSCLAQSAALCLGTWAAGPAQARSTRRAVVINMALE
                     PDSLDPTMAAAAAVGEVTHYNVLEGLTRIEENGAVAPLLAESWSVDGTGRHYHLRLRQ
                     GVRFHDGAAFDAAAVRFSFERAVAPSATNKARKALFDNIASIGTPDAHSVALTLHHPD
                     PQLLFRLGEGPAVMLHPATAAQAATRPVGTGPYQVAQWRRGHGIELAQAPMYRQAARL
                     RMHQASFRFLHDPLAQNAALRSGEIDVAFNFATQTVRRFQDETGYQVLIGASTGKGLL
                     ALNQRRAPLNDVRVRRAITHAIDREGFIRSVLDGRGVAIGSHFSPADAGYLHLANMYP
                     HDPERARALLKSAGVATPLRLTLALPPAPYAHEGGPVIAQDLARVGIVAELQRLSWEQ
                     WLNGPFKGQFDMTLINHVEPLDYRIYADPGYYFGYDSPAFRDLVQRHGAADNARERQL
                     LWAELQRQLARDAVNAWIFAPQVGTVVRKGLRGTWMNYPIFVHDIAAMWWE"
     gene            2152..4131
                     /locus_tag="PK518_02710"
     CDS             2152..4131
                     /locus_tag="PK518_02710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM47850.1"
                     /translation="MKAPDTMPPHHARSRAWLLLLLVALLLGGMAWGLSHVYQEREQR
                     YRHQIEGSLQAVNQLQLRAVTDWRARRMAEAAALTQDSLFARAVAQWRGAPSSPQQAA
                     LRERLTILMEQVRYTAAYLVDAQGRLLLDAQGAVTGQLPEPEQQALQLALAQARPVVV
                     ELRRDPAFAFAFYGLIAPLFDGTRALGAVWLLVDARATLYPLLETWPNHSPTAESVLV
                     QRSGDEVVSLSPLPLRASESGALRLPLAQGGRDPMALAATGVRGAFYARDYRGQEVLA
                     VASAVADSPWLLVSKVDVGDAFTDAQRREWLGLSLFVSLGLLSLGLVVLLWQWRAWRR
                     ERALKRELERNLRWLDNAQKAATVGYFTYETRSEAFVMSRMASAIFGLPDEGRMSLRQ
                     WMTMLHPDESVQTLRIHGQAMMERTPLRTQYRILRHGDRQERWVEVWGEYSQASENEP
                     LLMTGTVQDITERKHTEEQLARYRTALEAQVRLDPLTQVANRLALHEAVTQEWSRAQR
                     EGQPLALVMIDVDHFKAYNDSYGHVAGDQCLQQVATAMSALLGRAGDLLARYGGEEFA
                     VLLPGADAPAAMAVALRLQDAVRQLALPHRGSACCDSVTLSMGVTSLQAAHYPGADAA
                     QALFQQADAALYTAKQTGRNRVVLFGADCMQALHS"
     gene            4171..4830
                     /locus_tag="PK518_02715"
     CDS             4171..4830
                     /locus_tag="PK518_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM47851.1"
                     /translation="MMFDAILFDCDGVLVDSEPITNGVLCQMLNEAGWPLPPEECMRL
                     FIGKTVRSETARIEARTGRPLTDAWMAEFYARRNARLQAELVAIPNAVETVRAVHARL
                     QGRIACASGADRQKVEMQLAQVGLAPLFAGRIFSGHEMPATKPAPDVYLAAAAALGVP
                     PARCLVVEDTVTGVQAGVAAGATVVGFSSDGVGHGSPAALRAAGAQWVLSDMALLTKM
                     I"
     assembly_gap    5097..5196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5197..7749
                     /locus_tag="PK518_02720"
     CDS             <5197..7749
                     /locus_tag="PK518_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM47852.1"
                     /translation="AGHQGRLFDSRSGFGLRLQGLQPGAPVLCRANFVGPARLSVLQW
                     DEASLQTAIQAFEHNAGLLEGGIMMLVLFVFITALINRNPGYVLFAVWLLINLRMGAL
                     SAGWDQRWLGHDMPVAWLLQGRPITLALLYVLTFALFRTLFREELAKCAASGVLIWLQ
                     WSCALLLLLSATLSYAQFLPLIWVATVVGSMLLVIILGRIIAITRSPVAAWYGAAIAV
                     MLLSSLYEVVAAAFGLQAWIGSVNSVTAALASSLLATMAIAAQMRQEHQQRIQAQAEL
                     QHSYEVTPLGLFTLDLEGRFIAANPALHAALGPTVLARGNNHWDQYIASMDWLRMLQA
                     LNQQPAVEFELAGRPGARAPKGRRFLVKASRSGARVEGSLQDVTERSLATERLQFLAH
                     HDPLTQVLNRDGIRAELDWALQSAQTGPALALAYLDLDRFKLINDLFGHYAGDEVLRQ
                     VCTRVTALLQPGMHMGRMGGDEFIILMPGLGLAQATALCQGIVDQIGHLPYRVGDRSF
                     HVRGSVGLIEAAPGSSAKDAISTADRACREAKQSSGSHLVVFEHDSRALQEHEAEILL
                     AGQLTASDELQGLYLEMQPIMSLREPEHSLNFEVLLRMRDAAGQSVPTPRLITAAENS
                     GRMGLIDRWVLRQTLAWMRAHQARLRHTRFICMNLSGASLNDESFLHDAVQLLQRYAD
                     VAPRLCLEITESVALHDLENTRRFIAQVHGLGAKVAFDDFGAGYTSFSYLKQLKGDLL
                     KIDGSFIVNMNEHPANLAIVEAIVGLARNLGMKTIAEWAEDSATVQALAEIGVDYVQG
                     FAIARSQAPERILAARSAADFVSDPGLLHSLVLQAWPAPQPPSGLPPGWVAH"
     gene            complement(7753..8538)
                     /locus_tag="PK518_02725"
     CDS             complement(7753..8538)
                     /locus_tag="PK518_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47853.1"
                     /translation="MLSPALMPSLPAMPLPPIMTRGMHDNQAPARVEERLPFSVRVVS
                     DAEALDKAVQIRHAAYARHVPDLARTLLQPEAADFSAGSVVLLAQSHLDGSPLGTMRI
                     QTNRYEPLALEQSLALPDDLSGCLLAEATRLGVAEGRVGTLVKTVLFKAFYLYCLHSG
                     IDWMVVTGRAPVDRQYLRMLFEDVYPDIGYIPMRHVGNLPHRVMKFEVRTAQERWAAV
                     HHPMLKFMVETHHPDISLDAPQASQADMQATAPLLQQAPAQLM"
     assembly_gap    8771..8870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9104..9898
                     /locus_tag="PK518_02730"
     CDS             9104..9898
                     /locus_tag="PK518_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HRM47854.1"
                     /translation="MIKRRDCLGAALLGPWAVGPLWAAPDRTGGDPLGSMQWPSLHEQ
                     YLDKAPVRFTDAVLVRTPAFADDALNVPLQIDARALADVGGGIARMLVLADRNPVREV
                     LEFEPLRSLPMLAFRMRLEQASPIRALVQTRDGQWHAGGAMVQAAGGGCTVPGATRAD
                     GSWRTTLNQVRARIFNNVIEGSRRLRVNIMHPMDTGLVAGIPAFYIERLALLDAQGEP
                     WWRLQLHEPVSENPLLSFELPAQGPQAFHLSGQDNNGNRIAAEVRA"
     gene            9895..10872
                     /locus_tag="PK518_02735"
     CDS             9895..10872
                     /locus_tag="PK518_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HRM47855.1"
                     /translation="MKAAALLLAACAMAAGAQTPPAPPTPAQPDMASLDYRLQPRRIA
                     ENTWVIEGAVADFSRANGCNIINTAFIATGEGVLVINTGPSRLYGEQQRRAIAAVTDQ
                     PVRQVLNLNLHPDYFFGNQAWADVPIQALAGSIEGQRAEGEAYADNLYRLCGDWMRAT
                     QATPAREALAPQTLRLGRHVLHLQRLQGHTGDDLVLLDEHTGVLFAGGLVFANRVPTT
                     PHADAGAWLASLDQLRQWHAQGRFRLVVPSHGPVHAGLQGMEQTRDWLQWLTALMQTS
                     AAQGLDLSELLRQPVPERFAAWAAQPAELHRSLAQWYPRYERQALGARP"
     gene            11036..11359
                     /locus_tag="PK518_02740"
     CDS             11036..11359
                     /locus_tag="PK518_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM47856.1"
                     /translation="MRNLILLAFLSAAGAAAHASTDLAAAAEIAKSNGCYSCHAAAEK
                     IVGPAFNSVAEKYAGDKDAVATLAQSIQMGSKGKWGRAAMPAHSSLSAQDLKLLAGWV
                     LSSKP"
     gene            11368..11580
                     /locus_tag="PK518_02745"
     CDS             11368..11580
                     /locus_tag="PK518_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47857.1"
                     /translation="MAATHPHTVLGAAWAMLQPAADAQGQHLQAHAACLLGDYQRARL
                     LWERLAQSGDGEAQLHLGQLAQRRTA"
     gene            complement(11823..13091)
                     /locus_tag="PK518_02750"
     CDS             complement(11823..13091)
                     /locus_tag="PK518_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HRM47858.1"
                     /translation="MARLQRRQLLLGAAGLAAPAILRAQGANARVVVVGGGFGGATVA
                     RYLRAWAPRVQVTLVEPAERFVTCPFSNHYLAGLRSWDSISHGYDGLRAAGVQVIHAR
                     AEDVDGPRRSLRLHTGQELPWDRLVLSPGVDMRWGALEGYDEAAAELAPHAWKAGAQT
                     RLLRRQLEAMPDGGLFVMVIPDNPFRCPPGPYERASLVAHYLQQHKPRSKILLLDAKN
                     NFSKKELFLPAWKTLYGDRIEWVSLAEDGQVVRVDARALEVETLFGTRHRADVLNVIP
                     PQKAGLIAARAGVLDASGWAPVRGENFASKQVAGIHVLGDAALAGPMPKSGFAANNQG
                     KLVAAAIAAELTDQRAPTAWYANTCYSLLAPDYGISVAGVYRAEQGRLVDLPHSVGVS
                     PLDAPPAHRAAEARHGAAWYAAICADIWDR"
     gene            complement(13091..13453)
                     /locus_tag="PK518_02755"
     CDS             complement(13091..13453)
                     /locus_tag="PK518_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47859.1"
                     /translation="MAQTLIQQAHTAIKNSAFLAAGLLLPALCTAQSAPSPQDTALLA
                     GNCITCHGPGGQPPAGAGSSIPALRGQSAARLLERMQAFQAGQVADATVMPLLMQGYD
                     KTQMQALARWFAQPAEKP"
     gene            complement(13477..14610)
                     /gene="pqqE"
                     /locus_tag="PK518_02760"
     CDS             complement(13477..14610)
                     /gene="pqqE"
                     /locus_tag="PK518_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HRM47860.1"
                     /translation="MAGSINTPTPPVGVGQPLWLLAELTYRCPLHCVFCYNPVQLKQA
                     SSELTTAEWVEVMRQARQLGAAQLGFSGGEPLVRDDLEELVQEAHRLGYYTNLITSGV
                     GLTPARAQRLKDAGLDHVQLSFQDSTRELNDFLSHTKTFELKKKVAQTIKAHDWPMVM
                     NCVLHRHNLPHVGQIIEMALDLGAEYLELANVQYYGWAWVNRDHLMPTRAQLQEAEAV
                     VQGWRERIGKQCRLLFVVPDYFEERPKACMNGWGSVFLSIAPDGLAMPCHNARDLPGL
                     QLPNVREQPIADIWQRSQAFNAYRGQDWMQEPCRSCDERYKDFGGCRCQAFMVTGDAA
                     ATDPVCSKSPQHEKIVEFVRRAPERRQSIPIVHRSDPQPCARA"
     gene            complement(14594..14896)
                     /gene="pqqD"
                     /locus_tag="PK518_02765"
     CDS             complement(14594..14896)
                     /gene="pqqD"
                     /locus_tag="PK518_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HRM47861.1"
                     /translation="MNFDAAAHAAAPPYPDAPQLPHLSRRLRLQYEAAQTRWVLLYPE
                     GMVQLNDSAAEILRRCDGRHTVADIVNELESLFDTQGIAPQVHELLAEGTRRGWLD"
     gene            complement(14886..15623)
                     /gene="pqqC"
                     /locus_tag="PK518_02770"
     CDS             complement(14886..15623)
                     /gene="pqqC"
                     /locus_tag="PK518_02770"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HRM47862.1"
                     /translation="MDIAISPAPHPSLPAWSAQEFEAQLRDKGAAYHIHHPFNVRMNA
                     GGCTADELRCWVANRFYYQICIPRKDAAILANMPDRAHRRLWVERILDHDGQGDHQGG
                     NAGGIEAWTRLGQAVGIEREDLWSLRRVAPAVRFACDAYVNFAARAPWQEAVCSSLTE
                     MFAPQIHKDRLATWPGHYPWIEAEGLHYFRSRIPLASRDVEHGLRVTLEHFTTRAAQQ
                     RALDILQFKLDVLWSMLDAIEKACHEL"
     gene            complement(15645..16562)
                     /gene="pqqB"
                     /locus_tag="PK518_02775"
     CDS             complement(15645..16562)
                     /gene="pqqB"
                     /locus_tag="PK518_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HRM47863.1"
                     /translation="MLLRVLGSSAGGGFPQWNCNCPNCAGLRAGSLRARARTQSSVAV
                     TADGQDWLLVNASPDLLTQIQQTPTLQPARSLRDSGIAAVLLMDAQIDHVTGLLMLRE
                     RKTPLPLLATPEVLSDIGAGFPITQILSHYCGVAAQAIVCDGRSFAAPGLPGIALQAV
                     TLSSKPPPYSPFRGAPRPGDNIGLMVTNPATGARLFYAPGLAEVTPDLLALMARCSVV
                     LVDGTFWTNDEMQRLGLSNKAALDMGHLPQSGAGGMLDQLARLPRSVRKVLIHINNTN
                     PILNEDSPERAELAAQGVELAFDGMEIEF"
     gene            complement(16620..16694)
                     /gene="pqqA"
                     /locus_tag="PK518_02780"
     CDS             complement(16620..16694)
                     /gene="pqqA"
                     /locus_tag="PK518_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HRM47864.1"
                     /translation="MQWTTPAFTDLRFGFEITMYIANR"
     gene            16777..17832
                     /locus_tag="PK518_02785"
     CDS             16777..17832
                     /locus_tag="PK518_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM47865.1"
                     /translation="MPRKINATLDVPHWPAPRRLARGGLHRMAWRDMGRGEPWLLLHG
                     GPGSGCSPALLAPFDLARQRVIAPDQRGAGDSRPSGRTAANHLAALVADLEALRRHLG
                     LERWSLLGGSWGTVVALAYARQHPDHVARLVLRGAFGLTRSEVGGLLLPSARPGKRVA
                     QGDWPVPHGAALPQALSGLSQLLQSGAMTVPSLQASRGWALRERRDALHGLRRGLRHL
                     SGKDATAQRRLWAGLQRQQRRALAQLPRPRATPADRQAQARYRLQAHYLRHRGFVRPG
                     ELDAAVLALARHGVPVDWVHGRFDAICPPGNSRRWAAMGRPHARLQLVASGHLGAEPA
                     MLRALRYCVAAAVGDSD"
     gene            complement(17796..18224)
                     /locus_tag="PK518_02790"
     CDS             complement(17796..18224)
                     /locus_tag="PK518_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRM47866.1"
                     /translation="MEQIKPQVDTSALPALGWHEPNPLRGNAEAAAIGKAAFNQSCAV
                     CHGQDAIGTRSPAPDLRRIGMGCRRIQDAALRQRCQGDADAFFIKSVRYGKQKFGIVH
                     MPPWEGLLAPELAWALRSFVETAPKGTGIQSLSPTAAATQ"
     gene            complement(18307..18906)
                     /locus_tag="PK518_02795"
     CDS             complement(18307..18906)
                     /locus_tag="PK518_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRM47867.1"
                     /translation="MTRFTRIALTGAILLAGAGAALAQPAQDDARSEPLVINGCPIWP
                     YTRCPGADLRHASLAGKNLAGADLRGANLTRADLRGANLSAADLEGANLTGARMSKVS
                     AANTDFKGAKFIGTDLESARLMRSDFSGALFEGTSLEMARMNHAWFVGTRFIANDFQE
                     TKFVATNMQNAFFDGNHTLYTIFTESNLDGCQGCPPDWR"
     gene            complement(18995..20884)
                     /locus_tag="PK518_02800"
     CDS             complement(18995..20884)
                     /locus_tag="PK518_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HRM47868.1"
                     /translation="MTSSVSKQLRLSSLAVAATLALTAHAASVKPVTWEDIANDHKTT
                     ADVLTYGLGLTAQRYSPAKTLNTKNVNGLVPVWSYSFGGEKQRGQEAQALIHDGVMYV
                     TASYSRFVALDVRTGKRLWTYEHRLPEDIRPCCDVVNRGPAIFGDKVYFGTLDARVVA
                     LDRNTGKVVWNEKFGDHKVGYTMTGAPFIIKDQKTGKTLLVHGSSGDEFGVVGWLYAR
                     DPDTGKEVWARPMVEGHVGRLNGQPSTPTGDPKAPSWPNDPNSPTGKVEAWSQGGGAP
                     WQTPSFDVETNTIIVGTGNPAPWNTWKRTAKGDDPRNWPSLYTSGQAYVDPSTGDLNG
                     FFSHTPNDAWDFSGNNSVLLFDYKDAKSGKTIKASAHADRNGFFFVTDREKLAKADGG
                     HPWKQNAIIGAYPFVDGITWAKGFDLKTGLPIENNNRPPEPKEGQEKGDSIFVSPPFL
                     GGTNWMPMSYSPDSGLFYIPANHWAMDYWSEQITYKPGAAYLGQGFRIKKLYDDHVGI
                     LRAINPQTGKIAWEHKEQFPLWAGTLTTAGGLLITGTSDGFVKAFDNKTGKELWKFQT
                     GSGIVSVPVTWEMDGEQYIAIQSGYGGAVPLWGGDMAELTKQVSQGGSMWVFKLPKAQ
                     KVAAK"
     gene            complement(21053..21577)
                     /locus_tag="PK518_02805"
     CDS             complement(21053..21577)
                     /locus_tag="PK518_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRM47869.1"
                     /translation="MQRNRYDRFSVTLHWLMAVLLLGQIALGMWMLGLPKDASGLRAS
                     WFNVHKSWGMVLGLLIALRLAWALWRPRVAALPQPRPMQLLATGAHRLLYLLMLLMPL
                     SGFVGSVFSGYPIRFFGLRLPQLAERWDGAKELASGVHQWSAWALVLLIALHVLAFVH
                     HQVIRKEPLLQRMR"
     gene            complement(21602..>22462)
                     /locus_tag="PK518_02810"
     CDS             complement(21602..>22462)
                     /locus_tag="PK518_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRM47870.1"
                     /translation="RQPFVAVNCASIPDTLIEAELFGYEEGAFTGARKKGAAGKILQA
                     HGGTLFLDEIGDMPRHLQARLLRVLQERKVSPLGAAKEVEVDVTIISATHKNLKELIA
                     RGDFREDLYYRLNGLVVRLPALRERSDFEAVVKKVLHSLCESCANITIAPQVMELLQA
                     YHWPGNVRQLHNLLRTAAVMVDDDGIIRLEHLPDDFLDELRTPLRSPMVAPPVRAAEP
                     PLMPSVGAPPVPDDMADDSQSLQDVTINAMAQMLRLHKGNVSAAAKALGVSRNTIYRK
                     KNLLPPDLLD"
     assembly_gap    22463..22562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22563..23287)
                     /locus_tag="PK518_02815"
     CDS             complement(<22563..23287)
                     /locus_tag="PK518_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRM47871.1"
                     /translation="MDAQSGRPAAIAVQAPPEQDAHLVIDEAHQRSKAFGVCSDDAPD
                     FSSLAISQLKAVLEENRFLFQHAVPVMETLYGQIANTHSMVLLTSAQGMVLHSMGDND
                     FLEKANRVALVPGMDWSEKTKGTNAIGTALSAQQAVTVHGDQHFMSANQVLTCSCAPI
                     LDPYGQIVGALDVTGDHRSYHQHTIALVRMSAQMIENHMFAGSFPKAVRVHFHSRPEF
                     LGTLVEGIAAFTPEGRFLSANRSA"
     gene            23624..24109
                     /gene="pedF"
                     /locus_tag="PK518_02820"
     CDS             23624..24109
                     /gene="pedF"
                     /locus_tag="PK518_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HRM47872.1"
                     /translation="MQRSFLAPLQRSLLALALACGASAAFAHGDMVPQAVDTSTLPQL
                     GAKWLESNPYSSGEGHKEALRIGSSAYNQNCARCHGLEAVSGGIAPDLRKFDEECSGY
                     ADAAKKKVCFQEMDDFYAGTVRRGRTRDGRVYMPPFEGTLTQEAVWAIRTYLEERRAQ
                     Q"
     gene            complement(24275..25303)
                     /locus_tag="PK518_02825"
     CDS             complement(24275..25303)
                     /locus_tag="PK518_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HRM47873.1"
                     /translation="MPPHTRLLAGASAAAALALMAGCAQNPSAGPADNTDPTALVNQL
                     EATTGKFPGQRRSGAKGICATGEFVGSAQGRALSTASAFSGQPVPVVARFSLTGANPA
                     APDNTKSQRNMALQFNLPGGEQWLMGNISTPVFGAATPAQFLGRLASLQADPATKKAD
                     PAKVKAFADANPEVLLQGKYLAAQPVPASFAGLNYWGVHGFGFVNAQGATTWGKWLFE
                     PVGGVQGLSDDEAKAKGANFLFDDLRQRVATGQARFDFNLELAQSGDQLDNPTVPLPA
                     GRQKVNLGQLRITKVEAEGQGACMGVTFIPTVLPKGVVAGNDPVLAARAAPYAISQGR
                     RQSEAGAK"
     gene            complement(25393..27525)
                     /locus_tag="PK518_02830"
     CDS             complement(25393..27525)
                     /locus_tag="PK518_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRM47874.1"
                     /translation="MHFDQVCAGAGWPLRIATLSGLMALAQGAAAQTSPALPEVTVRS
                     TLVESPLEYSPASVTIVDGERVRDRQWQVNLSESLSTTPGLLLQNRQNYAQDLQLSIR
                     GHGARSTFGVRGVQVFVDGIPATMPDGQGQTNNIDLQSVERIEVLRGPYSTLYGNASG
                     GVINAYTERGEGRPRIDSSFAVGSNGQKRLGLKAQGEQSGVGYVVSASRYLTDGWRAQ
                     SAADKNMFNARLDTNPGDSGHLMLVANYVDVDALDPGGVTPADWQANARAVAQGPIDY
                     NMRKSMRQTQVGLSYDHRVDASNALRLMVYAGQRHITQYQSTPRAAQAPATSAGGVID
                     LGRDYGGMDLRWAHDTRLADSPFSLVAGLAVNVVEEDRQGYNNFLGSALGVKGALRRD
                     ERNTLNNADPYMQASWSFAPRWRLDAGLRWSNVKFESRDHYIAPGNGDGSGDTRYRRW
                     LPMLSLQHQISAATQVYASAGGGTETPTFNEISYRPGGLPGLNFGLQPATSRSLELGL
                     RQRLDGDALRGHWSAALFHTDTENEIVTADNTGGRATYRNAGRTRRQGAELGTQLQLA
                     RQWQLSGALTLLDAKLRDGFCNAAGTSCVSAGKRIAGTARTQAALGLDWRPHGDWRLG
                     ADWRHVSAIAANDSNSVEAPSYNVLGLSASHTRQWGAWKLNAFARVDNVTDKKYVGSV
                     IVNEGNGRFYEAAPGRQWMAGISLGYQF"
     gene            complement(27644..29170)
                     /locus_tag="PK518_02835"
     CDS             complement(27644..29170)
                     /locus_tag="PK518_02835"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRM47875.1"
                     /translation="MDMFEFKRFNLDLSFPYRQQYDNFIGGKWVAPAAGRYFDNVSPI
                     TGKPFCKVAQSDATDINLALDAAHAAQEKWGKTSVAERSGILLKIADRMEQNLELLAI
                     AETIDNGKPLRETMAADLPLGIDHFRYFAGCIRGQEGTISEIDANTVAYHFHEPMGVV
                     GQIIPWNFPILMACWKLAPALAAGCSIVMKPAEQTPASILVLMELIADLLPPGVVNIV
                     NGYGREAGEALATSKRIAKIAFTGSTPVGQRILHAAADNLIPSTVELGGKSPNIFFAD
                     VMDADDEFFDKALEGFSMFALNQGEICTCPSRILVQESIYERFMERAVARVQAMKQGH
                     PLDKSTMVGAQVSQAQLDRILGYIDIGRGEGAKCLTGGERLRAGGEINDGFFIKPTLL
                     YGQNDMRVFQEEIFGPVASVTTFKDVQDAVRIANDTEFGLGAGVWSRNGSVAYHMGRA
                     IQAGRVWTNCYHLYPAHAAFGGYKKSGIGRETHKLALSNYQQTKNLLVSYNPKALGFF
                     "
     gene            complement(29349..>30005)
                     /locus_tag="PK518_02840"
     CDS             complement(29349..>30005)
                     /locus_tag="PK518_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRM47876.1"
                     /translation="HCVSARSFRPLWLLEELGIPYRLHMLPFPPRVHARDYLARNPLG
                     TVPLLEDGATRLTESAAMCHYLATRYGAGVLDVAPADAAYGSYLNWLFMSDATLTFPQ
                     TLVLRYSYFEPPDRLQPQVAADYARWFLARLRAVDAALQHEPYLCAGRFTAADVAVTY
                     ALHLAQHLELAAQFPPAVADYWQRMQARPAFQRALQAQHQAALDQAVDPTPSPDLRPA
                     "
BASE COUNT         4518 a         9703 c        10453 g         5031 t
ORIGIN      
        1 gtgccagatc tacgagggca cgagcgacgt gcagaagatc atcatccagc gcgcgctggc
       61 ctgagggccg ggcccgttca tgcctgccgc cagcgcactg cccgcctgcc cctgcggccg
      121 caccgacagc cgccggcgtg cgctgccgta tgcgcaatgc tgcggccgct atatcgacca
      181 tttcgcgcaa tgcccggcgc ccgatgccga gagcctgatg cgctcgcgct acacggcctt
      241 cgtgcgtgag gacgcggcct acctgctggc cacctggcag gccgcgcacc ggccggcgca
      301 gctcgacttc gagcctggtg cgcgctggct gggcctgcag gtgcgcgccc atctgccact
      361 ggacgcgacc cacgccgagg tggaattcgt cgcgcgccag cgcgacgcca cgggccgggc
      421 acaccgcctg cacgagcgca gccgcttcct gcgcgagcag gggcgctggt actacgtaga
      481 tggaaaactt atataaatag tggctgtaac gcttgtatgg caagcgcagc acgctatcaa
      541 atttaaagca ctactcccac cacatggcag caatgtcgtg cacgaagatg gggtagttca
      601 tccaggtgcc gcgcagcccc ttgcgcacca cggtgcccac ctggggcgcg aagatccagg
      661 cgttgacggc atcgcgcgcc agctggcgct gcagctcggc ccacagcaac tggcgctcgc
      721 gtgcgttgtc ggcggcgcca tggcgctgca ccaggtcgcg gaatgccggg ctgtcatagc
      781 cgaagtagta gcccgggtcg gcgtagatcc ggtagtccag cggctccaca tggttgatca
      841 gcgtcatgtc gaactggccc ttgaacgggc cgttcagcca ttgctcccag gacagtcgct
      901 gcagctcggc cacgatgccg acgcgggcca ggtcctgcgc gatcaccggg ccgccctcgt
      961 gcgcgtaggg cgccggcggc aaggccaggg tcaggcgcag cggcgtggca acgccggcgc
     1021 tcttgagcag ggcgcgggcg cgctccgggt catgggggta catgttggcc aggtgcagat
     1081 accctgcatc cgccgggctg aagtggctgc caatggccac gccgcggccg tcgagcacgc
     1141 tgcgtatgaa gccctcgcgg tcgatggcgt gggtgatggc gcggcgcacg cgcacatcat
     1201 tgagcggtgc gcgccgctgg ttcagcgcca gcaggccctt gccggtggag gcgcctatca
     1261 gcacctggta gccggtttca tcctggaagc gacgcacggt ctgcgtggcg aagttgaacg
     1321 ccacgtcgat ctcgccggag cgcagcgcgg cgttctgcgc cagggggtcg tgcagaaagc
     1381 gaaagctggc ctggtgcatg cgcagccttg ccgcctggcg gtacatgggc gcctgggcca
     1441 gctcgatgcc gtggccacgg cgccactgcg ccacctggta gggcccggtg ccgacggggc
     1501 gggtggcggc ctgggccgcc gtggccgggt gcagcatcac ggccggcccc tcgcccagac
     1561 ggaacagcaa ctgcgggtcc gggtggtgca aggtgagggc cacgctgtgc gcgtccggcg
     1621 tgccgatgct ggcaatgttg tcgaacaagg ccttgcgcgc cttgtttgtc gcgctggggg
     1681 ccacggcgcg ctcgaagctg aagcgcacgg ccgctgcatc gaaggccgcg ccgtcgtgaa
     1741 agcgcacgcc ctggcgcagg cgcaggtggt aatgccgccc cgtgccatcc acgctccagg
     1801 actcggccaa cagcggcgcc accgcgccgt tttcctcgat gcgggtcaga ccctccagca
     1861 cgttgtaatg cgtcacctcg cccacggccg ccgccgcggc catggttggg tccaggctgt
     1921 cgggctccag tgccatattg atcaccacgg cgcgccgggt ggagcgggcc tgggcggggc
     1981 cggcggccca tgtgcccagg catagcgcgg ccgactgggc caggcaggag cggcgggtaa
     2041 gggcagttgc tggcatggta ttggggtatt gttgcggaga gatacaaact gtgacataac
     2101 atccggggtt tgacgacccc ttctcctgtt ttcattcgcc cgcctcttgt catgaaagcg
     2161 cccgacacca tgccgccgca ccacgcgcgc tcgcgcgcat ggctgctgtt gctgttggtg
     2221 gccctgctgc tgggcggcat ggcctggggg ctgtcgcatg tctaccagga gcgcgagcag
     2281 cgctaccgcc accagatcga ggggagtctg caggctgtca accagctgca gctgcgcgcc
     2341 gtcaccgact ggcgtgcgcg gcgcatggcc gaggctgcgg cgctgacgca ggacagcctg
     2401 tttgcccggg ccgtggcgca gtggcgcggc gcgccgtcat cgccccagca ggcggcgctg
     2461 cgcgagcgcc tgaccatcct catggagcag gtgcgctaca ccgcggccta cctggtcgat
     2521 gcgcagggcc ggctgctgct ggacgcccag ggcgccgtca ccggacagct gccagagccc
     2581 gagcagcagg ccctgcagct ggcgctggca caggcccggc cggtcgtcgt cgagctgcgc
     2641 cgcgacccgg cgtttgcctt tgccttctac ggcctgatcg cgcccctgtt cgatggtacg
     2701 cgtgcgctgg gcgccgtgtg gctgctggtg gatgcgcgcg ccaccctgta tccgctgctg
     2761 gagacctggc ccaaccacag ccccacggcc gaatccgtgc tggtgcagcg cagtggcgac
     2821 gaggtggtct cgctcagccc gctgcccctg cgcgccagcg agtcaggggc gctgcgcctg
     2881 cccctggcgc agggtgggcg cgaccccatg gccctggccg ccaccggggt gcgcggcgcc
     2941 ttttacgcgc gggactaccg cggccaggag gtgctggccg tggccagtgc cgtggccgac
     3001 tcgccctggt tgctggtgtc caaggtggat gtgggcgatg ccttcaccga tgcccagcgc
     3061 cgtgaatggc tgggcctgag cctgtttgtc agcctgggtt tgctgtcgct gggcctggtc
     3121 gtgctgctgt ggcagtggcg cgcctggcgg cgcgagcgtg ccttgaagcg cgagctcgaa
     3181 cgcaacctgc gctggctgga caacgcgcag aaggccgcca ccgtgggtta tttcacctat
     3241 gagacacgca gcgaggcctt tgtcatgtcg cgcatggcca gcgccatctt cggcctgccc
     3301 gacgagggcc gcatgagctt gcgccagtgg atgaccatgc tgcacccgga cgagagcgtg
     3361 caaaccctgc ggatccacgg ccaggccatg atggagcgca cgccgctgcg cacccagtac
     3421 cgcatcctgc gccacggtga ccggcaggag cgctgggtgg aggtctgggg ggaatacagt
     3481 caggcctcgg aaaatgaacc gctgctcatg accggcacgg tgcaggacat caccgagcgc
     3541 aagcacaccg aagagcagct ggcgcgctac cgcacggcgc tggaggcgca ggtgcgcctg
     3601 gacccactga cccaggtggc caaccgcctg gcgctgcacg aggccgtgac ccaggagtgg
     3661 agccgagccc agcgcgaggg ccagccgctg gcgctggtga tgatcgacgt ggaccatttc
     3721 aaggcctaca acgacagcta cggccatgtc gcgggcgacc aatgcttgca gcaggtggcg
     3781 acggccatgt cggccctgct gggccgcgcg ggcgacctgc tggcgcgcta tggcggcgaa
     3841 gagttcgccg tgctgctgcc cggcgctgat gcccccgctg ccatggccgt cgccctgcgg
     3901 ttgcaggatg ccgtgcgcca gctggcactg ccgcaccgtg gcagcgcctg ctgcgacagc
     3961 gtgaccctga gcatgggcgt gaccagcctg caggcggcgc actatccggg cgccgatgcc
     4021 gcgcaggccc tgttccagca ggccgacgcc gccctgtaca cggccaagca gaccgggcgc
     4081 aaccgcgtcg tgctgtttgg tgcagactgc atgcaggccc tgcattcata gccctggtgg
     4141 gccaggggca accacccaac cccatgaccc atgatgtttg acgcgatctt gtttgactgt
     4201 gacggcgtgc tggtggacag cgagccgatc accaacggcg tgctgtgcca gatgctcaat
     4261 gaggccggct ggccgctgcc gcccgaggaa tgcatgcgcc tgttcatagg caagaccgtg
     4321 cgcagcgaga cggcgcgcat cgaggcccgg accggccgcc cgctgaccga tgcctggatg
     4381 gccgagttct acgcccgccg caacgcgcgc ctgcaggccg agctggtggc cattccgaat
     4441 gccgtggaga ccgtgcgcgc cgtgcatgcg cggctgcagg ggcgtattgc ctgcgcctcg
     4501 ggtgccgatc ggcagaaggt ggagatgcag ctggcacagg tcggcctggc gcccctgttt
     4561 gccgggcgca tcttcagcgg ccatgaaatg ccggccacca aacccgcgcc cgacgtctat
     4621 ctggccgccg ccgcggccct gggcgtgccg cccgcgcgct gcctggtggt cgaggacacg
     4681 gtcaccggcg tgcaggccgg ggtggccgca ggcgccaccg tggtgggctt cagctcggac
     4741 ggcgtgggcc acggctcgcc cgctgcgctg cgggctgcag gggcgcagtg ggtgctcagc
     4801 gacatggcct tgttgaccaa aatgatttaa gttgcaagca gaatcgaacg aaaataacaa
     4861 aaagagatca attgccaagg ttggtactca ctaagccgct attccgccat ccctgacatt
     4921 cctgcccaca gatatcgcac gtgaagcctt cttccacccc cgccgcgcta cccttctgga
     4981 gccgtttgca tggtgggctg gatcacgtct tgacctggct gctcatctat gccctgcccg
     5041 gcgccgtgct ggtgttgtcg ttgtgggcct tgttcacctg ggcgccgcac tttgccnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg gccatcaggg gcggttgttt
     5221 gactcgcgca gcggctttgg cctgcggctg cagggcctgc agcccggcgc gccggtgctg
     5281 tgccgcgcca actttgtcgg cccagcgcgc ttgagcgtgc tgcagtggga cgaggcctcg
     5341 ttgcagacgg ccatccaggc cttcgagcac aacgccggcc tgctggaggg cggcatcatg
     5401 atgctggtgt tgttcgtctt catcaccgcc ctgatcaacc gcaacccggg ctatgtgctg
     5461 tttgcagtgt ggctgctgat caatctgcgc atgggcgccc tgtccgccgg ctgggatcag
     5521 cgctggctgg ggcatgacat gccggttgcc tggttgctgc aagggcgtcc catcaccctg
     5581 gcgctgctgt atgtgctgac ctttgcactg tttcgcacgc tgttccggga agagctggcg
     5641 aaatgcgccg cgtcaggggt gctgatctgg ctgcaatgga gctgcgcgct tttgctgctg
     5701 ttgtccgcca cgctgtccta tgcgcagttt ctgccgctga tctgggtggc cacggtcgtc
     5761 ggcagcatgc tgctggtgat cattctgggg cgcatcatcg ccatcacgcg ctcgccggtg
     5821 gcggcctggt atggcgcggc aattgccgtg atgttgctgt ccagcctgta tgaggtggtg
     5881 gcagcggcct ttgggctgca ggcctggatt ggctcggtca acagcgtgac ggcggcgttg
     5941 gcatccagcc tgctggccac catggccatc gccgcgcaga tgcgccagga gcaccagcag
     6001 cgcatccagg cgcaggccga gctgcagcac agctacgagg tgacgccgct ggggctgttc
     6061 acgctggatc tggagggccg cttcatcgcc gccaaccctg cgctgcatgc agcgctgggg
     6121 cccaccgtgc tggcgcgggg gaacaaccac tgggatcaat acatcgcctc catggactgg
     6181 ctgcgcatgc tgcaggcgct gaaccagcag ccggccgtgg agtttgagct ggcggggcgg
     6241 ccaggcgcgc gcgcgcccaa ggggcgccgt tttctggtga aggcctcgcg ctccggcgcg
     6301 cgtgtcgaag gctctttgca ggatgtgacc gagcgctcgc tggccacgga gcgcctgcag
     6361 ttcctggccc accatgaccc gctgacccag gtgctcaatc gcgacggcat tcgcgccgag
     6421 ctggactggg cgctgcagtc cgcgcagacc ggcccggccc tggcactggc ctatctcgat
     6481 ctggatcgct tcaagctgat caatgacctg tttggccact acgccggcga cgaggtgctg
     6541 cgccaggtct gcacccgggt gacggcgctg ctgcagcctg gcatgcacat ggggcgcatg
     6601 gggggcgatg aattcatcat cctcatgccc ggcctgggcc tggcgcaggc tactgccctg
     6661 tgccagggca tcgttgacca gattggccat ctgccctatc gggtgggtga ccggtctttt
     6721 catgtgcgcg gctccgtggg tttgatcgag gcggcgcctg gcagctcggc caaggatgcg
     6781 atctccaccg ccgaccgtgc ctgccgcgag gccaagcaaa gcagcggcag ccacctggtg
     6841 gtgtttgagc atgactcgcg tgcgctgcag gagcatgagg ccgagatact gctggccggc
     6901 cagctgaccg cgtcggacga gctgcagggc ctgtacctgg agatgcagcc catcatgtcg
     6961 ctgcgcgagc ccgagcactc gctcaacttc gaggtgctgc tgcgcatgcg cgacgccgcc
     7021 ggccagagcg tgcccacgcc gcgcctgatc accgcggccg agaacagcgg ccgcatgggc
     7081 ctgatcgacc gctgggtgct gcgccagacc ctggcctgga tgcgcgcgca ccaggcgcgc
     7141 ctgcgccata cgcgtttcat ctgcatgaac ctcagcggcg cttcgctgaa cgacgagagt
     7201 tttctgcacg atgccgtgca gctgctgcag cgctacgccg acgtggcgcc gcgcctgtgc
     7261 ctggagatca ccgagagcgt ggccctgcac gacctggaaa acacccggcg cttcatcgcc
     7321 caggtgcatg gcctgggcgc caaggtggcg tttgatgatt ttggcgccgg ctacacctcg
     7381 ttttcctacc tcaagcagct caagggcgac ctgctcaaga tcgacggcag cttcatcgtc
     7441 aacatgaacg agcacccggc caatctggcc atcgtcgagg ccatcgtcgg cctggcacgc
     7501 aacctgggca tgaagaccat tgccgaatgg gccgaagaca gcgccacggt ccaggcgctg
     7561 gccgagattg gcgtggacta tgtgcagggc tttgccatcg cccgctcgca agcgcccgag
     7621 cgcatactcg cggcgcgttc ggcggcagac tttgtgagcg acccgggcct gctgcattcc
     7681 ctggtgttgc aggcctggcc cgcgccacag ccgccctcgg gcttgccacc ggggtgggtc
     7741 gcccactgaa acctacatca gctgcgccgg ggcttgctgc agcaggggcg ctgtggcctg
     7801 catgtccgcc tgggatgctt gcggtgcatc caggctgatg tcagggtggt gcgtttcgac
     7861 catgaatttg agcatcgggt ggtgcacggc ggcccagcgc tcctgggccg tgcgcacctc
     7921 gaacttcatc acgcggtgcg gcagattgcc gacatgccgc atggggatgt agccgatgtc
     7981 cgggtacacg tcctcgaaca gcatgcgcag atactggcga tcgaccggcg cgcggcccgt
     8041 caccaccatc cagtcgatgc cgctgtgcag gcagtacagg taaaaggcct tgaacagcac
     8101 ggtcttcacc agggtgccca cgcggccctc ggccacgccc aggcgggtgg cctcggccag
     8161 caggcagccg ctcaagtcgt caggcagggc cagcgactgc tccagcgcca gcggctcgta
     8221 gcggttggtc tgtatgcgca tggtgcccag gggcgagcca tcgagatggg actgggccag
     8281 cagcaccacg gagccggcgg aaaagtcggc cgcttcaggc tgcagcaggg tgcgcgccag
     8341 atccggcaca tggcgcgcgt aggccgcgtg gcgtatctgc accgccttgt ccagcgcctc
     8401 ggcatcgctg accacgcgca ccgaaaacgg caggcgttcc tcgacccggg ccggggcctg
     8461 gttgtcgtgc atgccgcggg tcatgatggg cggcaggggc atggcgggca gggaaggcat
     8521 cagggctggt gacaacatga tcaggtctcc cggcctgtcg gacatgcagg ccatgtactc
     8581 cactatcggg cgcggggcgc aacgcgatgc gtggatgacg gggtgaaaca accctctttt
     8641 ccggcggcat gaagatggcc gggccgaggc gcgcagagcc ggtgaacaca ttccccgcgg
     8701 atatatggac tggcggtgca acccctcccc gtcgcacgca tgctgagcgc gcttcatgtt
     8761 ttgctgggtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgggtgaca
     8881 cattgctgcg cagtgaacct gttcacaggt ttgaattgat tctgtccagc ccgcgggcct
     8941 ttcctggtaa gcgttggtaa ctatcaattt gtgagtattg tcacgacagc gacaagccgg
     9001 ccttgtgccc tggcgggcag gcatggcgat tcttctggca ctgcttttgc ttgctgcccc
     9061 agggttgcgc ccgcgtggcg gtttgttgga aagggcactg atcatgatca agcgcaggga
     9121 ttgcctgggg gcggcgttgc tcggcccctg ggccgtaggg ccgctgtggg cggcgccgga
     9181 tcgcaccggg ggcgaccccc tgggctccat gcaatggccc agcctgcacg agcagtatct
     9241 ggacaaggca ccggtgcgct ttaccgacgc cgtgctggtg cgcacgccgg cctttgccga
     9301 cgatgcgctc aacgtgccct tgcagataga tgcccgcgcc ctggcggatg tgggtggcgg
     9361 cattgcgcgc atgctggtgc tggccgatcg caacccggtg cgcgaggtgc tggagttcga
     9421 gccgctgcgc tccctgccca tgctggcgtt tcgcatgcgg ctggagcagg cctcgccgat
     9481 acgtgcgctg gtgcagacgc gcgacggcca atggcatgcg ggcggtgcca tggtgcaggc
     9541 ggccggcggg ggctgcaccg tgcccggcgc cacgcgcgcc gacggttcct ggcgcaccac
     9601 gctgaaccag gtgcgcgcgc gcatcttcaa caacgttatc gagggcagcc ggcgcctgcg
     9661 cgtgaacatc atgcacccca tggacacggg gctggtggcc ggcataccgg cgttctacat
     9721 tgagcgcctc gcgctgctgg acgcgcaggg cgagccctgg tggcgcctgc agctccacga
     9781 gccggtgtcg gaaaacccgc tgctgagctt tgagctgccg gcccagggcc cccaggcatt
     9841 tcacctgagc gggcaggaca acaacggcaa ccgcatcgcc gccgaggtgc gcgcatgaag
     9901 gccgccgccc tgttgctggc cgcctgcgcc atggccgccg gcgcgcagac gccccctgcg
     9961 ccgcccaccc cggcccagcc cgacatggcc agtctggact accgcctgca gccgcgccgc
    10021 attgccgaga acacctgggt catcgagggc gcggtggccg atttctcgcg tgccaacggc
    10081 tgcaacatca tcaacaccgc cttcatcgcc accggcgagg gcgtgctggt gatcaatacc
    10141 ggcccctcgc gcctgtacgg cgagcagcag cgccgcgcca tcgccgccgt gacagaccag
    10201 cccgtgcgcc aggtgctgaa cctgaacctg caccccgact acttctttgg caaccaggcc
    10261 tgggccgatg tccccatcca ggcgctggcc ggcagcattg agggtcaacg cgccgagggc
    10321 gaggcctatg ccgataacct ctaccgcctg tgtggcgatt ggatgcgcgc cacccaggcc
    10381 acaccggcgc gtgaggcgct ggcgccgcag accttgcgcc tggggcgcca tgtgctgcac
    10441 ctgcagcgcc tgcagggcca cacgggtgac gacctggtgc tgctggacga gcacaccggc
    10501 gtgctgtttg ccggcggcct ggtgtttgcc aatcgcgtgc ccaccactcc ccatgccgat
    10561 gccggcgcct ggctggccag cctggatcag ctgcggcagt ggcacgccca ggggcgcttt
    10621 cggctggtgg tgcccagcca cgggccggtg catgccggcc tgcagggcat ggagcagacg
    10681 cgcgactggc tgcaatggct caccgccctg atgcagacca gcgccgcgca ggggctggac
    10741 ctgagcgagc tgctgcgcca acccgtgccc gagcgctttg ccgcctgggc cgcgcagccg
    10801 gccgagctgc accgcagcct ggcgcagtgg tacccgcgct acgagcggca ggccctgggc
    10861 gcacggccgt gaagctgtga cacgcactgc ttcatttttg aacagttggc gcgcgcactg
    10921 gcacgcatat ggacagcagc ggcggcatcc gcagcggggc ggggctggca tggatgttgc
    10981 ccctatcagc agcaagcaag ccaaacctct ttgaatccac ctgaagggcc ccaccatgcg
    11041 caacctcatc ctcctcgcct tcctgtcggc cgctggcgca gccgcccacg ccagcaccga
    11101 cctggccgcg gccgccgaga tcgccaagtc caatggctgc tacagctgcc acgccgcggc
    11161 cgagaagatc gtcggcccgg ccttcaattc ggtggccgag aagtacgccg gcgacaagga
    11221 cgccgtggcc acgctggcgc agtccataca aatgggctcc aagggcaaat ggggccgcgc
    11281 cgccatgccg gcgcattcca gcctcagcgc gcaggatctg aagctgctgg ccggctgggt
    11341 gctcagcagc aagccatgag ccgggccatg gccgccaccc atccgcatac cgtgcttggc
    11401 gccgcctggg ccatgctgca gccggcggcc gatgcccagg gccagcactt gcaggcccat
    11461 gccgcctgcc tgctgggcga ctaccagcgc gcgcgcctgc tgtgggagcg gctggcgcaa
    11521 agcggcgacg gtgaggcgca gctccacctg gggcagctgg cacagcgccg aaccgcttga
    11581 ggttcaacct aaaaaccgca gttgaccgct tgtaatcctt ggaaaagtcg catggaatct
    11641 gactttgtgc tacttgggat cgttcacctt ttgggtgaga gcgctatgcg cccgccagca
    11701 ccgcgctcga cgcgccatgc ggcgttgctt ctccttgcgc acaggagtgc gctgcgtcgt
    11761 cgcgccttgc ctggcacgcc gatcacggca ctggcgggcg catccaactg cggtttttag
    11821 gttcaacgat cccagatatc ggcgcagatg gccgcgtacc aggctgcgcc atgccgcgcc
    11881 tcggcggcgc ggtgggcggg tggggcgtcc agcgggctca cgcccacgct gtgcggcaga
    11941 tccaccaggc gcccctgctc ggcgcggtag acgccggcca ccgagatgcc gtagtccggc
    12001 gccagcaggc tgtagcaggt attggcgtac caggcggtgg gggcgcgctg gtcggtcagc
    12061 tcggcggcaa tggccgccgc caccagcttg ccctggttgt tggcggcaaa gccggacttc
    12121 ggcatcggcc cggccagggc cgcgtcgccc agcacatgga tgcctgccac ctgtttggaa
    12181 gcaaaatttt ctccccgcac cggcgcccag ccgctggcat ccagcacgcc tgcgcgcgcg
    12241 gcaatcaggc cggccttctg cggcgggatg acgttgagca catcggccct gtggcgcgtg
    12301 ccaaacagcg tctccacctc cagcgcccgg gcatcgacgc gcaccacctg gccatcctcg
    12361 gccaggctga cccattcgat cctgtcgccg tacaaggtct tccaggcggg cagaaagagt
    12421 tctttcttgg aaaagttgtt cttcgcatcc agcagcagga tcttgctgcg tggcttgtgc
    12481 tgctgcaggt aatgcgcgac caggctggcg cgctcatacg gcccgggcgg gcagcggaac
    12541 gggttgtccg ggatcaccat cacgaacagg ccgccgtcgg gcatggcctc aagctggcgg
    12601 cgcagcagcc gggtctgggc gccggccttc caggcatgcg gcgcgagttc ggcggctgcc
    12661 tcgtcgtaac cctccagcgc gccccagcgc atgtccacgc cgggcgagag caccaggcga
    12721 tcccagggca actcctggcc ggtatgcagg cgcaggctgc gccgcgggcc atccacgtcc
    12781 tcggcccggg cgtggatgac ctgcacgccg gcggcgcgca ggccgtcgta gccgtggctg
    12841 atgctgtccc agctgcgcag cccggccagg taatggttgg agaacgggca ggtgacaaag
    12901 cgctcggccg gctccaccag ggtcacctgc acccgtggcg cccaggcgcg caggtagcgc
    12961 gccaccgtgg cgccgccaaa gccgccgccg accaccacca cgcgggcgtt cgcgccctgg
    13021 gcgcgcagga tggcaggagc cgccaggccg gccgcgccca gcaataactg gcggcgctgc
    13081 agcctggcca tcagggcttc tccgctggct gggcgaacca gcgcgcgagg gcctgcatct
    13141 gggtcttgtc atagccctgc atcaacagcg gcatgacggt ggcgtctgcc acctgcccgg
    13201 cctggaaggc ctgcatgcgc tcgagcaggc gggcggcgct ttggccgcgc agggcgggaa
    13261 tgctgctccc ggcccccgct ggtggctggc cgccggggcc atggcaggtg atgcagttgc
    13321 cggcgagcag ggccgtgtcc tgtgggctcg gggcggactg cgccgtgcag agtgctggca
    13381 gcagcagacc ggcggccaga aacgcactgt ttttgatagc tgtatgcgct tgctggataa
    13441 gcgtttgagc catattttgt ttgaaatatg gcggattcag gcgcgtgcac atggctgcgg
    13501 gtcgctgcgg tggacgatgg ggatgctctg cctgcgctcg ggcgcacggc gcacgaactc
    13561 gacgattttt tcgtgctgtg gcgatttact gcataccggg tcggtcgcgg cggcgtcgcc
    13621 cgtgaccatg aaggcctggc agcggcagcc gccaaagtcc ttgtagcgct cgtcgcagct
    13681 gcggcagggc tcctgcatcc agtcctggcc acggtaggcg ttgaaggcct ggctgcgctg
    13741 ccagatgtcg gcgattggct gctcgcgcac gttcggcagt tgcaggccgg gcaggtcgcg
    13801 cgcgttgtgg cagggcatgg ccaggccgtc gggggcgatc gacaggaaca ccgagcccca
    13861 gccgttcatg caggccttgg ggcgctcctc aaaataatcg ggcacgacga acagcagccg
    13921 gcattgttta ccgatgcgct cgcgccagcc ctgcactacg gcctcggcct cttgcagctg
    13981 cgcgcgcgtg ggcatcaggt ggtcgcggtt cacccaggcc cagccgtagt actgcacgtt
    14041 ggccagctcc aggtattcgg cgcccaggtc gagggccatc tcaatgattt gcccgacatg
    14101 cggcaggttg tgccggtgca gcacacagtt catgaccatg ggccagtcgt gcgccttgat
    14161 ggtctgcgcc acctttttct tcagctcgaa ggtcttggtg tggctcagaa aatcgttcag
    14221 ctcgcgcgtg ctgtcctgga aggacagctg cacatgatcg agccccgcgt ccttgaggcg
    14281 ctgcgcccgc gccggcgtca ggccgacgcc cgaggtgatg aggttggtgt agtagcccag
    14341 gcggtgcgcc tcctgcacca gctcctccag gtcgtcacgc accagcggct cgccgcccga
    14401 gaacccgagc tgcgccgcgc cgagctggcg cgcctggcgc atcacctcga cccattcggc
    14461 ggtcgtcagc tcgctgctcg cctgcttgag ctggaccggg ttgtagcaaa acacgcagtg
    14521 cagcgggcag cggtaggtca gctcggccag cagccacagc ggctggccta cgccgaccgg
    14581 cggcgtgggg gtgttaatcg agccagccac ggcgtgtgcc ctcggccagc agctcgtgca
    14641 cctgcggggc gatgccctgg gtgtcgaaca gcgattcgag ctcattgacg atgtcggcca
    14701 cggtgtggcg gccgtcgcag cggcgcagga tctcggcggc gctgtcgttc aattgcacca
    14761 tgccttcggg gtagagcagc acccagcgcg tctgcgcggc ctcgtactgc aggcgcaggc
    14821 ggcgcgacaa gtgcggcagc tgcggcgcgt cggggtaggg cggagctgct gcgtgggcgg
    14881 cggcgtcaaa gttcatggca ggctttctct atggcatcca gcatggacca gagcacgtcc
    14941 agcttgaatt gcagaatgtc cagcgcacgc tgctgtgcgg cgcgggtggt gaaatgttcc
    15001 aaggtcacgc gcaggccatg ctcgacgtcg cggctggcca gcggaatgcg gctgcgaaag
    15061 tagtgcaggc cctcggcctc tatccagggg taatgcccgg gccaggtggc caggcggtcc
    15121 ttgtggatct gcggtgcgaa catctcggtg agcgacgagc acacggcctc ctgccaggga
    15181 gcgcgggcgg caaagttgac ataggcgtcg caggcaaagc gcacggccgg cgccacgcgg
    15241 cgcagtgacc acaggtcttc gcgctcaatg cccacggcct ggcccaggcg tgtccaggcc
    15301 tcgatgccgc cggcattgcc gccctggtgg tcaccctggc cgtcatggtc caggatgcgc
    15361 tccacccaca ggcggcggtg cgcgcgatcg ggcatgttgg ccaggatggc ggcgtccttg
    15421 cgcggtatgc agatctggta atagaagcga ttcgctaccc agcagcgcag ctcgtccgcg
    15481 gtgcagccgc cggcgttcat gcgcacattg aacgggtggt ggatgtggta ggccgcgccc
    15541 ttgtcgcgca gctgggcttc gaactcctgc gcgctccagg cgggcaggct tgggtggggg
    15601 gcggggctga tggcgatgtc catggtgggc agtcctgtcc agaatcagaa ttcaatttcc
    15661 atgccatcga aggccagctc cacgccctgc gcggccagct cggcgcgctc gggcgagtcc
    15721 tcgttcagta tggggttggt gttgttgatg tggatgagca ccttgcgcac gctcctgggc
    15781 aggcgcgcca gttgatcgag catgccgccg gcgccgcttt gcggcagatg ccccatgtcc
    15841 agcgccgcct tgttggacag gcccaggcgc tgcatttcgt cattcgtcca gaaggtgccg
    15901 tccaccagca ccacactgca gcgggccatc aaggccagca gatccggtgt gacctcggcc
    15961 aggccggggg cgtagaacag gcgcgcgccg gtcgccgggt tggtgaccat caggccgatg
    16021 ttgtcgcccg ggcgcggcgc gccgcgaaac ggcgaatacg gcggcggctt ggacgacagc
    16081 gtcacggcct gcaaggcgat gcccggcagg cccggggcgg caaaactgcg cccgtcacag
    16141 actatggcct gggcggccac gccgcagtaa tgggaaagaa tctgggtgat gggaaagccc
    16201 gcgccaatgt ccgacaacac ctcgggtgtg gccaagagcg gcagcggcgt cttgcgctcg
    16261 cgcagcatca agagacccgt gacatggtcg atctgtgcgt ccatcagcag caccgcggca
    16321 atgccgctgt cgcgcaggct gcgcgccggt tgcagcgtcg gcgtctgctg gatttgcgtc
    16381 agcaggtccg gcgaggcgtt gaccagcagc cagtcctgcc catcggccgt gacggccacg
    16441 ctggattggg tgcgtgcccg ggcgcgcagg ctgcctgcgc gcaggccggc gcaattgggg
    16501 cagttgcagt tccactgcgg aaagccaccg ccggcggaag agcctaaaac gcgcaatagc
    16561 atggtctgtg aatgtgggtg atgtgcgggg gcgacgcctg ccgcccccgc caggggggat
    16621 taacggttgg cgatgtacat ggtgatttca aagccgaagc gcagatcggt gaaggcgggg
    16681 gtggtccatt gcatggttgt ctcctgtgat gagtggcatg aaagatgatt gcgtgccgca
    16741 caaaacccgt agaacctgtt tcacgcagcc gcagccatgc cacgcaaaat caatgccacc
    16801 cttgatgtac ctcattggcc cgcgccgcgc cggctggcgc gtggcgggct gcaccgcatg
    16861 gcctggcgcg atatgggacg gggcgaaccc tggctgctgc tgcatggcgg cccgggcagc
    16921 ggctgcagcc cggccctgct ggcaccgttt gacctggcgc gccagcgtgt gatcgcgcca
    16981 gaccagcgcg gcgcgggcga ctcgcgcccc agcgggcgca cggcggccaa ccatctggcg
    17041 gcgctggtgg ccgacctgga ggcactgcgc cggcatctgg gcctggagcg ctggtcgctg
    17101 ctgggtggct cctggggcac ggtggtggcc ctggcctatg cgcgccagca cccggatcat
    17161 gtggcgcggc tggtgctgcg cggcgccttt ggcctcacgc ggagcgaggt gggcggccta
    17221 ctgctgccca gcgcgcggcc cggcaagcgc gtggcgcagg gcgactggcc ggtaccgcac
    17281 ggggccgcac tgccccaggc cctgtccggc ctgtcacaac tgctccaaag cggagcaatg
    17341 actgttccat cgctgcaggc cagccggggc tgggcgttgc gcgagcggcg tgatgcgctg
    17401 catggcctgc gccgcggcct gcggcatctg tcgggaaagg atgcaaccgc ccagcgccgg
    17461 ctatgggccg gcctgcagcg ccagcagcgc cgtgccctgg cgcagctgcc ccggccgcgc
    17521 gccacgccgg cggatcgcca ggcgcaggcc aggtaccgcc tgcaggcgca ttacctgcgc
    17581 caccggggct ttgtgcgacc cggagaactc gatgccgccg tgctcgccct ggcgcggcat
    17641 ggggtgccgg tcgattgggt gcatgggcgc tttgacgcca tttgcccgcc cggcaacagc
    17701 cggcgctggg cggccatggg ccggccccat gcccgcctgc agctggtggc cagcgggcac
    17761 ctgggcgcag agcccgccat gctgcgggcg ctgcgctact gcgtggcggc ggcggtgggc
    17821 gacagcgact gaatacccgt gcccttgggc gcggtttcga caaagctgcg cagcgcccag
    17881 gccagctcgg gcgcaagcag gccttcccag ggcggcatgt ggacgatgcc gaacttctgc
    17941 ttgccgtagc gcaccgactt gatgaagaac gcgtccgcgt cgccctggca gcgctggcgc
    18001 agggccgcgt cctggatgcg ccggcagccc atgccgatgc ggcgcaggtc gggtgcgggc
    18061 gagcgtgtgc caatggcatc ctggccatgg cagacggcgc agctctggtt gaaggcggcc
    18121 ttgccgatgg cggcggcttc ggcattgccg cgcagggggt tgggctcgtg ccagccgagc
    18181 gccggcaggg ccgaggtgtc gacctggggc ttgatctgct ccacggcctg gacatttgtg
    18241 gcaaaaattg ccgctagccc aatgcaggta agcgcaagat gctttgaaaa atatagcatt
    18301 cgcatatcat ctccagtccg gtgggcagcc ctgacagcca tcgaggttgg attccgtgaa
    18361 gatggtgtag agcgtgtggt tgccgtcgaa gaatgcgttc tgcatgttgg tggcgacgaa
    18421 cttggtctcc tggaaatcat tggcgatgaa gcgcgtgccg acgaaccacg catggttcat
    18481 gcgcgccatc tccaggctgg tgccctcgaa cagggcgccc gagaagtccg agcgcatcag
    18541 gcgcgcgctc tccaggtcgg tgccgatgaa tttggcgccc ttgaaatcgg tgttggccgc
    18601 cgagaccttg gacatgcgcg cgccggtcag gttggcgccc tccagatcgg cggccgagag
    18661 attggcgccg cgcaggtcgg cgcgcgtcag gttggcgccg cgcaggtcgg cgccagccag
    18721 gttcttgccc gccaggctgg catggcgcag gtcggcgccg gggcagcggg tatagggcca
    18781 gatcgggcag ccgttgatga ccaggggttc gctgcgggcg tcgtcctggg ccggctgggc
    18841 cagcgccgcg ccggccccgg caagcaaaat cgccccggtc agggcgatgc gggtgaaacg
    18901 ggtcatgggc gtgggctccg ggtggttgat gggagcgccg gcgtctcgcc ggcacaggaa
    18961 atccatgccg gcgagacgcc ggcgctccca ggacttactt ggccgccacc ttctgcgcct
    19021 tgggcagctt gaagacccac atcgagccgc cctgcgagac ctgcttggtc agctcggcca
    19081 tgtcgccgcc ccacagcggc acggcgccac cgtagccgga ctggatggcg atgtactgct
    19141 cgccgtccat ctcccaggtc accggcacgg agacgatgcc cgagccggtc tggaacttcc
    19201 acagctcctt gccggttttg ttgtcgaaag ccttgacgaa gccgtccgag gtgccggtga
    19261 tgagcaggcc gccggccgtg gtcagcgtgc cggcccacag cgggaactgc tccttgtgct
    19321 cccaggcgat cttgccggtc tgcgggttga tggcgcgcag gatgcccaca tggtcgtcat
    19381 acagcttctt gatgcggaag ccctggccca ggtaggccgc gccaggcttg taggtgatct
    19441 gctcggacca gtagtccatg gcccagtggt tcgccgggat gtagaacagg ccgctgtccg
    19501 ggctgtagct catgggcatc cagttggtgc cgcccaggaa cgggggcgag acgaagatgc
    19561 tgtcgccctt ttcctggcct tccttgggct cgggcgggcg gttgttgttc tcgatcggca
    19621 ggccggtttt caggtcaaaa cccttggccc aggtgatgcc atcgacgaag gggtaggcac
    19681 cgatgatggc gttctgcttc caggggtggc cgccgtcggc cttggcaagc ttttcgcggt
    19741 cggtcacgaa gaagaagcca ttgcggtcgg cgtgcgccga ggccttgatg gtcttgccgc
    19801 tcttggcgtc cttgtaatcg aacagcagca ccgagttgtt gcccgagaag tcccaggcgt
    19861 cattgggcgt gtgcgagaag aagccgttca ggtcgccggt gctcggatcg acataggcct
    19921 ggcccgaggt gtagaggctg ggccagttgc gtgggtcgtc gcccttggcg gtgcgcttcc
    19981 aggtgttcca gggcgcgggg ttgccggtgc cgacgatgat ggtgttggtc tccacgtcga
    20041 acgagggcgt ctgccacggc gcgccgccgc cctggctcca ggcctcgacc ttgccggtgg
    20101 ggctgttcgg gtcgttgggc cagctgggcg ccttggggtc gccggtgggg gtgctgggct
    20161 ggccgttcag gcggccgaca tggccttcga ccatggggcg ggcccagact tccttgccgg
    20221 tatccgggtc gcgggcgtag agccagccga ccacaccgaa ctcgtcgccc gaggagccat
    20281 gcaccagcag ggtcttgccg gtcttctgat ccttgatgat gaagggcgcg ccggtcatgg
    20341 tgtagcccac cttgtggtcg ccgaatttct cgttccagac caccttgccg gtgttgcggt
    20401 ccagggccac gacgcgcgcg tccagcgtgc cgaaatacac cttgtcgcca aaaatggccg
    20461 ggccgcggtt gaccacgtcg cagcagggac ggatgtcctc gggcaggcgg tgctcatagg
    20521 tccacaggcg cttgccggtg cgcacgtcca gcgccacgaa gcgcgagtac gaggcggtga
    20581 catacatcac gccgtcgtgg atcagcgcct gcgcctcttg gccgcgctgc ttctcgccgc
    20641 cgaaggagta gctccacacc ggcaccaggc cgttgacgtt cttggtattg agcgtcttgg
    20701 ccgggctgta gcgctgcgcc gtcaggccca ggccataggt cagcacgtcg gccgtggtct
    20761 tgtggtcgtt ggcaatgtct tcccaggtga cgggcttgac gctggcggca tgggccgtca
    20821 gcgccagggt ggcggccacg gccaggctgc tcaggcgcag ttgcttggat accgatgagg
    20881 tcatggtggt ttgctccttg tgttgtggtg ggtgagatcc ccgccacatc aacgcaagag
    20941 ccgtgccaca ttgacaatcg tcacgctgcc ggcccgggtg ccgtgtttgc cgttcaaatg
    21001 tgcaacgctg tgtggcgccg cggttgctca gctatggagc agccggtgac attcagcgca
    21061 tgcgctgaag cagcggctcc ttgcggatca cctggtggtg cacgaaggcc agcacatgca
    21121 gggcgatgag cagcaccagg gcccaggccg accattggtg cacgccgctg gccagctcct
    21181 tggcgccgtc ccagcgctcg gccagctgcg gcaggcgcag gccgaagaag cggatggggt
    21241 agcccgagaa caccgagccc acgaagcccg acagcggcat cagcagcatc agcaggtaca
    21301 gcagccggtg ggcgcccgtg gccagcagct gcatggggcg gggctgcggc agcgccgcca
    21361 cgcgcgggcg ccagagcgcc caggccaggc gcagcgcgat cagcaggccc agcaccatgc
    21421 cccaggactt gtgtacgttg aaccaggagg cgcgcaggcc gctggcatcc ttgggcaggc
    21481 ccagcatcca catgcccagg gctatctgcc ccagcagcag cacggccatg agccagtgca
    21541 gggtgacgga aaaacggtcg tatcggttgc gctgcacaaa agcctcctgt tgctccatcg
    21601 gtcaatcgag cagatcgggc ggcagcaggt ttttcttgcg gtagatggtg tttcgcgaca
    21661 cacccagggc cttggccgcc gccgacacat tgcccttgtg caggcgcaac atctgtgcca
    21721 tggcgttgat ggtcacatcc tgcaggctct ggctgtcatc ggccatgtcg tcaggcacgg
    21781 gcggggcccc cacgctgggc atcagcggcg gctcggccgc gcgcacgggg ggggcgacca
    21841 tggggctgcg caggggcgtg cgcagctcgt cgagaaaatc gtcgggcaga tgctccaggc
    21901 ggatgatgcc gtcatcgtcc accatcaccg ccgccgtgcg cagcaggttg tgcagctgac
    21961 gcacattgcc cggccagtgg taggcctgca gcagctccat aacctggggc gcgatcgtga
    22021 tgttggcgca gctctcgcac agcgagtgca gcaccttctt caccacggcc tcgaagtcgc
    22081 tgcgctcgcg cagggccggc aggcgcacca ccaggccgtt caggcggtag tacaggtctt
    22141 cgcggaagtc gccgcgcgcg atcagctcct tcaggttctt gtgcgtggcg ctgatgatgg
    22201 tgacatccac ctccacctcc ttggccgcgc ccagcgggct caccttgcgc tcctgcagca
    22261 cgcgcagcag ccgtgcctgc aggtgcctgg gcatgtcgcc gatctcgtcc aggaatagcg
    22321 tgccgccatg ggcctgcaat atcttgccgg ccgcgccctt cttgcgtgcg ccggtgaagg
    22381 cgccttcctc gtagccgaac agctcggcct cgatcaaggt gtcgggaatc gaggcgcagt
    22441 tcacggccac gaagggctgg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nngcgctgcg gttggccgag agaaagcgcc cctcgggcgt gaacgcggca atgccctcca
    22621 ccagggtgcc cagaaactcg ggacgcgaat ggaaatgcac gcgcacggcc ttcgggaagc
    22681 tgcccgcgaa catgtggttc tctatcatct gcgccgacat gcgcaccagg gcgatggtgt
    22741 gctggtggta gctgcggtgg tcgccggtca cgtccagcgc gccgacgatc tggccgtagg
    22801 ggtcgagaat cggcgcgcag gagcaggtca gcacctggtt ggcgctcatg aaatgctggt
    22861 cgccatgcac ggtgacggcc tgctgtgccg acagcgccgt gccgatggcg ttcgtgccct
    22921 tggtcttctc gctccagtcc atgccgggca ccagggccac gcggttggcc ttctccagaa
    22981 aatcgttgtc gcccatggaa tgcagcacca tgccctgggc cgaggtcaag agcaccatgc
    23041 tgtgggtatt ggcgatctgc ccatacagcg tctccatcac cggcaccgca tgctggaaca
    23101 gaaagcggtt ctcttccagc accgccttca gctggctgat cgccaggctg gagaagtcgg
    23161 gggcatcgtc gctgcagaca ccaaaggcct tggagcgctg gtgtgcctcg tcgatgacga
    23221 gatgtgcgtc ttgctctggc ggtgcttgca cggcgatggc ggccgggcgt ccagactggg
    23281 cgtccatgtt gtctcctggc gctggaagta ggttcttcca atggttttat gggtcctcct
    23341 gggcggcagg cgcagggggc gtggctccgg gcaagaacca agtattgtgc aaatggtacg
    23401 ggtgcctcgc aacccggatc cacagcaggg atcaagcaag aaatgtgccg caactgtgcc
    23461 actgaattgc tccatgctgt gacactcggc cgggtttgga cggcgccggc gggcacgcgc
    23521 tggcgcaaac tgggacagcg ccgggcccga acccgggcag cggcgggggt ggcatgcaaa
    23581 ttgcgcagct gagggcattc acttttccca caggagacaa accatgcaac gatcttttct
    23641 cgcccccctg cagcgcagcc tgctggccct ggccctggcc tgcggtgcct cggcggcatt
    23701 tgcgcacggc gatatggtgc cccaggccgt ggacaccagc acgctgcccc agctgggcgc
    23761 gaagtggctc gaatccaacc cctacagcag cggcgagggc cacaaggagg cgctgcgcat
    23821 aggcagctcg gcctacaacc agaactgcgc gcgctgccat gggctggagg cggtgtcggg
    23881 cggcatcgca cccgatctgc gcaagttcga cgaggaatgt tccggctacg ccgatgccgc
    23941 caagaagaag gtgtgcttcc aggagatgga cgacttctac gccggcaccg tgcgccgcgg
    24001 ccgcacgcgc gacggccggg tctacatgcc gccgttcgag ggcacgctga cgcaggaggc
    24061 ggtctgggcc atccgcacct atctggaaga gcgccgcgcg cagcagtgaa gacacgccat
    24121 tgcacaaaaa aaaggccgct gcgatagcgg cctttttact gggatgcggc gcttattccg
    24181 tttctacatc attcgtgtct aggcgcgaag ccgcagacag tgctgtagca cggcaaggcg
    24241 aagcaacaac gacacgggtg atttagaaat ggaattactt ggcgcccgcc tcgctctggc
    24301 gccggccctg ggatatggcg tagggcgcgg cacgcgcggc cagcaccggg tcgttgcccg
    24361 ccaccacgcc cttgggcagc acggtgggga tgaaggtcac gcccatgcag gcgccctggc
    24421 cctcggcctc gaccttggtg atcctgagct ggcccaggtt caccttctgg cgcccggcgg
    24481 gcagtggcac ggtggggttg tccagttggt cgccgctctg ggccagttcc aggttgaagt
    24541 caaaccgggc ctggccggtg gccacgcgct ggcgcaggtc gtcaaacagg aagttggcgc
    24601 ccttggcctt ggcctcgtcg tcgctcaggc cttgcacacc gccgacgggc tcgaagagcc
    24661 acttgcccca ggtggtcgca ccctgggcgt tgacaaagcc aaagccatgc acgccccagt
    24721 agttcaggcc ggcaaagctg gccggcacgg gttgcgccgc caggtatttg ccctgtagca
    24781 gcacctcggg gttggcgtcg gcaaaggcct tgaccttggc cgggtcggcc ttcttcgtcg
    24841 ccggatcggc ctgcagcgag gccaggcggc cgaggaactg cgccggcgtg gcggcgccga
    24901 acaccggcgt ggagatattg cccatgagcc actgctcgcc gcccggcagg ttgaactgca
    24961 gggccatgtt gcgctggctc ttggtgttgt cgggtgcggc ggggttggcg ccggtcagtg
    25021 aaaagcgcgc cacgacgggc accggctggc cgctgaaggc cgaggccgtg gacagcgcgc
    25081 ggccctgggc ggagccgacg aattcgccgg tcgcgcagat ccccttggcg ccggagcggc
    25141 gctggccggg gaacttgccg gtggtggcct cgagctggtt caccagggcc gtggggtcgg
    25201 tgttgtcggc tgggccggcg gacgggtttt gggcgcagcc ggccatcagg gccagggcgg
    25261 cggcggcgct ggcgccggcc agcagacggg tatgaggggg catgcgagga gtctccttgg
    25321 tggtggttat gagagaaaaa tggcttttgc gcaaactggg tatgcgcaaa cagctattaa
    25381 aaacatagtg actcaaaact gatagcccag gctgatgccg gccatccact gccgtccggg
    25441 cgccgcctcg tagaagcggc cgttgccctc gttgacgatg acggagccta catatttctt
    25501 gtctgtcacg ttgtccacgc gggcaaaggc gttcagcttc caggcgcccc attgccgggt
    25561 atggctggcg ctcaggccca gcacgttgta gctgggtgct tcgacgctgt tgctgtcgtt
    25621 ggcggcaatg gcgctgacat ggcgccagtc cgcgcccagg cgccagtcgc cgtgtgggcg
    25681 ccagtccagc ccgagggcgg cctgggtgcg cgccgtgccg gcgatgcgct tgccggcgct
    25741 cacgcagctg gtgccggcgg cgttgcagaa gccgtcgcgc agcttggcgt cgagcagcgt
    25801 cagggcgccg ctgagctgcc actggcgtgc cagctgcagc tgggtgccca gctcggcgcc
    25861 ctggcgccgc gtgcgcccgg cgttgcggta ggtggcgcgc ccgccggtgt tgtcggcggt
    25921 gacgatttcg ttctcggtgt ctgtatggaa cagcgccgcc gaccaatggc cgcgcagggc
    25981 gtcgccgtcc aggcgctggc gcaggcccag ctccaggctg cgcgaggtgg ccggctgcag
    26041 gccgaagttg agcccgggca ggccgccggg gcggtaggag atctcgttga aggtgggcgt
    26101 ctcggtgccg ccgccggccg aggcatagac ctgggtggcg gcgctgatct ggtgctgcag
    26161 cgacagcatg ggcagccagc gccggtagcg cgtgtcgccg ctgccgtcgc cattgccggg
    26221 ggcgatgtag tggtcgcgcg actcgaactt cacgttgctc cagcgcagcc cggcatcgag
    26281 cctccagcgc ggcgcaaacg accagctggc ctgcatgtag gggtcggcgt tgttcagcgt
    26341 attgcgctcg tcgcggcgca gcgcgccctt gacgccgagc gcgctgccca ggaagttgtt
    26401 gtagccctgc cggtcttcct cgaccacgtt cacggccagg ccggccacca ggctgaaggg
    26461 gctgtctgcc aagcgcgtgt catgcgccca gcgcagatcc atgccgccat agtcgcgccc
    26521 caggtcgatg acgccgccgg cactggtggc gggcgcctgt gccgccctgg gcgtggactg
    26581 gtactgggtg atgtggcgct ggccggcata caccatcagg cgcagggcat tgctggcgtc
    26641 cacgcggtgg tcgtagctca ggcccacctg ggtctggcgc atgctcttgc gcatgttgta
    26701 gtcgatcggc ccctgggcca cggcgcgggc attggcctgc cagtcggcgg gcgtgacgcc
    26761 gccggggtcg agcgcatcca catccacgta gttggccacc agcatcaggt ggccgctgtc
    26821 gcccgggttg gtgtccaggc gggcgttgaa catgttcttg tcggccgcgc tctgcgcgcg
    26881 ccagccgtcg gtcaggtagc ggctggcgct caccacatag cccacgccgc tctgctcgcc
    26941 ctgggccttc aggcccaggc gtttctggcc gttactgccg acggcgaagc tgctgtcgat
    27001 gcgcgggcgg ccctcgccgc gctcggtgta ggcgttgatc accccgcccg aggcgttgcc
    27061 atacagcgtg gaatagggtc cgcgcagcac ctcgatgcgc tcgaccgact gcaggtcgat
    27121 gttgttcgtc tggccctggc cgtcgggcat ggtggcgggt atgccgtcca cgaagacctg
    27181 cacgccgcgc acgccgaagg tcgagcgtgc gccatggccg cgtatcgaca gctgcaggtc
    27241 ctgggcgtag ttctggcggt tctgcagcag caggccgggc gtggtggaca gcgactcgga
    27301 cagattcacc tgccactggc ggtcgcgcac gcgctcgcca tcgacgatgg tcaccgaggc
    27361 cggcgaatac tccagcggcg actccaccag ggtgctgcgc accgtgacct cgggcagggc
    27421 cgggctggtc tgcgccgcgg cgccttgcgc cagggccatc aggccgctca aagtggcaat
    27481 gcgcaggggc cagccggcgc ctgcgcaaac ttgatcgaaa tgcatggaat gctccctccc
    27541 ttgttctgac caaagaggag cggggcgcac catcccgcac tgcttgcgcc ccgcgacatg
    27601 gatggcccgg cctgccgtgc cgcggcaggc cgggtggaca ggcctagaag aagcccagcg
    27661 ccttggggtt gtagctcacc agcaggttct tggtctgctg gtagttggac agggccagct
    27721 tgtgcgtctc gcggccaatg cccgacttct tgtagccgcc gaaggccgcg tgcgcggggt
    27781 agaggtggta gcagttggtc cacacgcggc cggcctggat ggcgcggccc atgtggtagg
    27841 ccacggagcc attgcggctc cacaccccgg cgcccaggcc gaactcggtg tcgttggcga
    27901 tgcgcacggc gtcctgcacg tccttgaagg tggtcaccga ggccacgggg ccgaagattt
    27961 cctcctggaa cacgcgcatg tcattctggc catagagcag cgtgggcttg atgaagaagc
    28021 cgtcgttgat ctcgccgccg gcgcgcaggc gctcgccgcc ggtcaggcat ttggcgccct
    28081 cgccacggcc gatgtcgatg tagcccaaga tgcgatcgag ctgagcttgc gacacctgcg
    28141 cgccgaccat ggtgctcttg tccagcgggt ggccctgctt catggcctgc acacgggcca
    28201 cggcgcgctc catgaagcgc tcgtagatgg attcttgcac caggatgcgc gacgggcagg
    28261 tgcagatctc gccctggttc agggcaaaca tggagaagcc ctccagcgcc ttgtcaaaga
    28321 actcgtcatc ggcgtccatg acgtcggcaa agaagatgtt cgggctcttg ccgcccagct
    28381 ccacggtgga ggggatcagg ttgtcggccg cggcgtgcag gatgcgctgg cccacggggg
    28441 tggagccggt gaaggcgatc ttcgcgatgc gcttgctggt ggccagggcc tcgccggctt
    28501 cacgcccgta gccgttgacg atgttcacca cgccaggcgg cagcaggtcg gcgatcagct
    28561 ccatcagcac caggatggag gcgggggtct gctcggccgg cttcatgacg atggagcagc
    28621 cggcggccag ggccggggcc agcttccagc aggccatcag gatggggaag ttccagggaa
    28681 tgatctgacc gaccacgccc atgggctcgt ggaaatggta ggccacggtg ttggcgtcga
    28741 tctcggaaat cgtgccttcc tggccgcgga tgcagccggc gaagtagcgg aaatggtcaa
    28801 tgcccagcgg caggtcggcg gccatggtct cgcgcagcgg cttgccgttg tcaatggtct
    28861 cggcgatcgc cagcagctcc aggttctgct ccatgcggtc ggcaatcttc agcaggatgc
    28921 ccgagcgctc ggccaccgag gtcttgcccc acttttcctg cgcggcatgg gcggcgtcca
    28981 gggccagatt gatgtcggtg gcgtcggact gcgccacctt gcagaacggc ttgccggtga
    29041 tgggcgagac gttgtcgaaa tagcggccgg cggccggggc cacccatttg ccgccgatga
    29101 agttgtcgta ctgctggcgg taggggaagg aaaggtcgag gttgaagcgc ttgaattcga
    29161 acatgtccat gttggtctcc ttgggtggtg gatgccggcg cacatgcgca ccgactgccc
    29221 caggagtaat caagcttcgt gccagcccgt tgcgtgccgg gtgtgccgac ttttgtcaca
    29281 gtccatggcg gtgggtgttc cagattggcc cactggtgtg caggcttgtg acaccgcagc
    29341 gcccggcatc aggccggccg cagatcgggc gacggcgtgg gatcgaccgc ctgatccagc
    29401 gcggcctggt gctgcgcctg cagcgcccgc tggaaggccg gcctggcctg catgcgctgc
    29461 cagtagtcgg ccacggccgg tggaaactgc gccgccagct ccaggtgctg tgccaggtgc
    29521 agggcatagg tcacggcgac gtcggcggca gtgaagcgtc cggcacacag atatggttca
    29581 tgctgcaatg cagcatccac cgcccgcagc cgcgccagga accagcgtgc gtagtccgcc
    29641 gccacctgtg gctgcagccg gtcagggggt tcgaaatagc tatagcgcag caccagcgtt
    29701 tgcggaaagg tcagcgtcgc atcgctcatg aacagccagt tgagatagct gccataggcc
    29761 gcgtccgccg gtgccacatc cagaacgccc gcgccgtagc gcgtggctag gtaatggcac
    29821 atcgcggccg actcggtcag ccgcgtggcc ccgtcctcca gcagcggcac cgtgcccagg
    29881 gggttgcgcg ccagatagtc ccgtgcatgc acgcgtggcg ggaagggcag catgtgcagg
    29941 cggtagggga tgcccagctc ttccagcagc caaagagggc ggaaggatcg ggcgctgacg
    30001 cagtg
//
LOCUS       DAOMTQ010000015        28318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_147_length_28318_cov_26.179599, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000015 DAOMTQ010000000
VERSION     DAOMTQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 28318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28318
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147_length_28318_cov_26.179599"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PK518_02845"
     CDS             <1..170
                     /locus_tag="PK518_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM47877.1"
                     /translation="LRAMPGVFCAGEMLDWEAPTGGYLLTACCATGRVAGAGAVHWLA
                     SPVPADQNLVR"
     gene            complement(152..709)
                     /locus_tag="PK518_02850"
     CDS             complement(152..709)
                     /locus_tag="PK518_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47878.1"
                     /translation="MVWVGAAVAQVADPLTSAASPPITLRGHSYPDFGALYPSDALRQ
                     PGAMSPQAIHYTDITGWLTPRQASSLGLTLGMVSYARGVELSPQAFDLGVRWRSQLDR
                     RVQLDIHAWARTSQLHAMPNAMGMIWHQDQPTFGTRVEVQWSSSRTRGLVPEFGAVGL
                     QLEGNSRLLLRARRGGPMLYYRTKF"
     gene            complement(961..1057)
                     /gene="ffs"
                     /locus_tag="PK518_02855"
     ncRNA           complement(961..1057)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK518_02855"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1136..3079
                     /gene="dnaX"
                     /locus_tag="PK518_02860"
     CDS             1136..3079
                     /gene="dnaX"
                     /locus_tag="PK518_02860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRM47879.1"
                     /translation="MSYLVLARKYRPKTFSEMVGQEHVVQALTNALTQQRLHHAYLFT
                     GTRGVGKTTVSRILAKSLNCQGPDGQGGITAQPCGVCPACQDIDSGRFPDYTELDAAS
                     NRGVDEVQGLLEQAVYKPVQGRFKVFMIDEVHMLTNTAFNAMLKTLEEPPEYLKFVLA
                     TTDPQKVPVTVLSRCLQFNLRPMAPETVLEHLTRVLAAEQVAAEPQALRLLSRAARGS
                     MRDALSLTDQAIAFGSGQLQEASVRLMLGSVDRSHVFRLIEALAAGDGRTVVETADAL
                     RVNGLSAASTLEEMAAVLQRMAVLQAVPQMAAAVDASDPEAAEIARLAAALPADETQL
                     LYSICLHGRTELGLAPDEYAALTMVLLRLLAFKPAGAAGATAEKKTLTPPEPAAPAPV
                     AVAADPPVAPLAVQPPIHVAREPQPPAPLAAPAAPEPATPDDVNRAVTEAMLQDAAAP
                     NAVPAALPGPAAAPTVRLPVRSPEDLRQKQHPAPANQAQTASETIAIPVRSAPEPGPR
                     LQAGAAAPPLVTTEEGDVWHATVQQLIAQDAITALVRELALQSQLMARDEGHWLLRVE
                     RESLNQPQARERLRAALEAAGHARQISVELGVVSDSPARRNAQAASARQRAAEDIVHN
                     DPYVQQLVRDFGAKIVPGSIKPA"
     gene            3118..3450
                     /locus_tag="PK518_02865"
     CDS             3118..3450
                     /locus_tag="PK518_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRM47880.1"
                     /translation="MFNKGQLAGLMKQAQAMQDNLKKAQDELASVEVEGESGAGLVKV
                     LMTCKHDVKRVTIDPSLLAEDKDMLEDLVAAAFNAAVRKAEETSQEKMGKITAGMPGL
                     PGGMKFPF"
     gene            3473..4063
                     /gene="recR"
                     /locus_tag="PK518_02870"
     CDS             3473..4063
                     /gene="recR"
                     /locus_tag="PK518_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRM47881.1"
                     /translation="MSDTNSLDALIQALRRLPGVGIKSAQRIAFHLLQHDRPAAQQLS
                     QALAQACAQVRHCQRCYTFTEGEVCATCMDATRDASRLCVVETPADQAAMERTAAFRG
                     LYFVLMGRLSPLDGVGPRDIGVQKLLERAGDGVVQEVILATSFTAEGEATAHAIAEAL
                     RRRGMHVTRLARGVPVGSELEYVDLGTIAHALADRR"
     gene            4102..4497
                     /locus_tag="PK518_02875"
     CDS             4102..4497
                     /locus_tag="PK518_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRM47882.1"
                     /translation="MSNTSFTLAYGCVLVAALLPMFCALLAKAGAMPRGGNRDPRAWL
                     AAQSGWRARANAAQANGFEGLPFFIGAVIIAHQLGAPQARLDQLACAFIVLRLAYIAL
                     YVGDKAMARSLVWGLGLAVNIAILLLGWR"
     gene            4624..5670
                     /locus_tag="PK518_02880"
     CDS             4624..5670
                     /locus_tag="PK518_02880"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HRM47883.1"
                     /translation="MIDLRGITQIYQGPQGPVEALRGIDLHVTPGEVFGIIGRSGAGK
                     SSLVRVINLLNRPSAGQVMVAGRDMTQLSDAELRAARREIGMVFQHFNLLSSRTVYGN
                     AALPLELAGMAPDKIKKRVDPLLDMVGLAHLADRYPAQISGGQKQRVGIARALASSPK
                     VLLSDEATSALDPETTRSILDLLRQVNRELGVTVVLITHQMQVIKQIADRVAVIEAGR
                     IVEMGPVIDVFTKPQQDITKSLIDEIVPQELPAGVLSRVRSLAARLEAGASGQLLRLS
                     YAGEHAYEPILSHLIRELGLDLSILHGQIDEIQEQTFGSLAVYASGPMARIGAAIDYL
                     RAQGVVVRTVDVSA"
     gene            5681..6346
                     /locus_tag="PK518_02885"
     CDS             5681..6346
                     /locus_tag="PK518_02885"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HRM47884.1"
                     /translation="MFENFSEMMLELFATSLWETVIMVGASGILGALVGIPLGVFLRL
                     TDKGGILQNAPANTVVGGIVNAVRSTPFIILLVAIIPFTRLVTGTSIGTWAAVVPLTL
                     AAAPFIARLVETALREVDGGLVEAAQSMGATTGQIVWKVLLPEAMPGIVAGLTISFVS
                     LTGYSAMAGAVGGGGLGDLGIRYGYQRFLPDVMLAVVVILIVFVQAIQSLGDWVVRRM
                     SHR"
     gene            complement(6400..7044)
                     /locus_tag="PK518_02890"
     CDS             complement(6400..7044)
                     /locus_tag="PK518_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRM47885.1"
                     /translation="MHAATHPIVFRDRLDAARRLAERLQAYAGQNPLVLAIPRGAVPM
                     AQCIAQALQGDLDLVLVRKLGAPYQPEYAIGSVDESGWTYLSPHAAATGADAHYIATE
                     KRRQLQLIHQRRAQYTPIRPPLEVARRIVIVVDDGLATGATMAAALHSLRQRKPARLI
                     CAVPVASHEALALVRPLADEVACLQAPQDFEAVGQYYEDFTQVEDDEVIQILSK"
     gene            7128..7928
                     /locus_tag="PK518_02895"
     CDS             7128..7928
                     /locus_tag="PK518_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM47886.1"
                     /translation="MISKRSLLQSTLALALATGLGGAAMAQDKPLKIGVTAGPHAQIF
                     EQVKKVADKQGLKIQVVEFSDYVQPNAALAAGDLDANSYQHKPYLDAQIKDRGYPFAV
                     AANTVNFPIGIYSKKIKKLADLKEGARFGIPNDPTNGGRVLLLLQAQGLIKLKDGAGL
                     KATPLDVVGNPKKLKFIELDAAQLPRSLDDLDASAINTNFAISAGLNPKTDAIAQENP
                     DGPYVNLLVVRAADKDKPWVAQLVKVYHSPEIRQFIDTQFKGSVLAGW"
     gene            8020..8571
                     /locus_tag="PK518_02900"
     CDS             8020..8571
                     /locus_tag="PK518_02900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HRM47887.1"
                     /translation="MSKYRIAVIVGSLRKDSHNRRLAHALTRLGAPEFEFHYPRIDDL
                     PLYNQDDDGHQPAPVLRLKQEVAESQGVLFVTAEYNRSIPGVLKNAIDNASRPYGHSA
                     WANKPAGVIGVSIGTIGTAMAQQHLRNVLVFLNMPTMAQPEAFIQNSDALFNADGSIG
                     EVSRAFLQAWMDSYVAWVKRHNA"
     gene            complement(8580..9989)
                     /locus_tag="PK518_02905"
     CDS             complement(8580..9989)
                     /locus_tag="PK518_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM47888.1"
                     /translation="MTGAAPDTPAASAELTPARRVLVFCIVSLALLMMSVDSTIVATA
                     LHALQQDLHTSVNWAGWSITAYAFGFVLMLPISGRLSDRYGRRRVFLGSVITFTAASL
                     ACGMADDIVTLVLLRALQAAGGAGFTPSATGILVDHFGQARDRAVGLFGTIFPIGAMI
                     GPIFGGLFVTYASWRDVFFVNVPIGLVVAVLALRYIPRDPPQGQQQRLRMDGAGMALL
                     AVTLFACMLVASYLGEAHAPVGPALLALLAGVAGVSGWLFLRHVARAEPPFISPRLIH
                     GPGFGVVSLVNMVYGGISIGAVALIPLYASNRYGMNALYAGTLLVAQGCASIVLSVLA
                     TMALRRTGYRLPLYVGGAISAVGMLLVALAPPAGMAPHAWLTGAALVLGLGTGAVNPA
                     SRNAGLQLAPQESSSIAGLRSLCFQLGTIAVVSLATAALAGAADAGMRQAWVYAAVAA
                     LLLLALPLVARVPEHHGAW"
     gene            complement(10050..10703)
                     /locus_tag="PK518_02910"
     CDS             complement(10050..10703)
                     /locus_tag="PK518_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM47889.1"
                     /translation="MQLLHIDSAITGHQSFSRQLTAQIVEAWQASHPATQVQYLDLVA
                     EAPAHFTMDAMAPRTGQTEGLSEAQQRENAVSERLVRQFLAADVVVIGAPFYNFSIPT
                     QLKAWIDRLAQPGRTFRYTANGPEGLAKGKTVIIASSRGGVYSTSDTGQAMEHQESYL
                     QTVLGFFGVTDVRFVRAEGVAMGADAKAQALAAAAQAIAAHVQHHEAANRERLAEVA"
     gene            10827..11741
                     /locus_tag="PK518_02915"
     CDS             10827..11741
                     /locus_tag="PK518_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM47890.1"
                     /translation="MQDLNDMLYFAEVAERGGFAAAGRALGIPKSRLSRRVAELEAQL
                     GVRLLQRTTRTLSLTEVGEAYLRHCQAMRESAQAAADAVAQVQSEPRGTVRVTCPVTL
                     AQTVLARLMPVFLERHPQVRLEMQVTNRVVNVVEEGVDVALRVRATLDNSGSMIVKRL
                     DEGRQLLVASPAQLARQGMPATLQDLAGMDTLAMSATDGRTSLRLTGPGGREETVQFT
                     PRYVADDLLTLKYAALAGTGLCWLPDYMCQHELQQGRLVQLLPEWSQPPGIVHAVFAS
                     RRGLSPAVRGFLDFLGETARGAKGDAPQ"
     gene            11837..12844
                     /locus_tag="PK518_02920"
     CDS             11837..12844
                     /locus_tag="PK518_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM47891.1"
                     /translation="MTTRIKFKTVFATLALAASGLASAQTQSLLNVSYDVAREFYKDY
                     NQAFIAHYKKTKGVDIKVDQAHAGSSAQARAVNDGLAADVVTFNTTTDVQFLADNGVV
                     AKDWAQKFPHEASPTTSTMLFLVRHGNPKNIKDWADLTRPDVKVIVVNPKTGGNGRYA
                     YLAAWGSVREKGGSDAQAAEFITQMYKNVPVLAKGGRDATATFLQRNIGDVLITFESE
                     VVSVEREFGKGKVDAVYPSVSITAKNPVAIVERTVAKKGTGELAKAYLDYLYSDEAQE
                     IAARHAIRPRSEAVLKKHADLFKPIPQFAVAKYFGSLVEAQKVHFNDGGQFDKLYQPG
                     K"
     gene            12891..13760
                     /gene="cysT"
                     /locus_tag="PK518_02925"
     CDS             12891..13760
                     /gene="cysT"
                     /locus_tag="PK518_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRM47892.1"
                     /translation="MGAASDLTGAAAPRRRPKRVLPGFGLTLGYTLFYLSIIVLIPLL
                     ALLAKSFSLSWPQFWEAVSAPRVLASYKLTFGASFIAACVNLVFGLLIAWVLVRYQFP
                     GKKIVDAFVDLPFALPTAVAGISLTALLAGNGWVGQYFEPFGIQLAFNPNGVVIALIF
                     IGLPFVVRTVQPVLEDSEKELEEAATSLGATRWQVFTKVILPSITPALLTGFAMAFAR
                     AIGEYGSVIFIAGNMPMVSEITPLIIIGKLEQYDTAGATAVAVVMLFFSFIMLLVINA
                     LQAWQRRHAGMPA"
     gene            13757..14650
                     /gene="cysW"
                     /locus_tag="PK518_02930"
     CDS             13757..14650
                     /gene="cysW"
                     /locus_tag="PK518_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HRM47893.1"
                     /translation="MSAVANPSTRRAQAGTTEAPWVRFTLIGLALAFLALFLLLPLAA
                     VFTEALRKGWDAYLAGLAEPDAWSAIRLTLLTAVIAVPLNLVFGVAAAWCIAKYEFKG
                     KAFLTTLIDLPFSISPVVAGLMYVLVFGANGWFGSWLAEHDIKIIFAVPGIVLATVFV
                     TFPFVARELIPLMQAQGNDEEQAAIVLGASGWQTFWHVTLPNIKWGLVYGVILCNARA
                     MGEFGAVSVVSGHIRGQTNTIPLHVEILYNEYQSVAAFAAASLLALLALVTLVIKSIA
                     EWQGEQQAKAAAALPPERPSA"
     gene            14696..15823
                     /locus_tag="PK518_02935"
     CDS             14696..15823
                     /locus_tag="PK518_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HRM47894.1"
                     /translation="MSIEIRNVSKQFGNFHALRDVNLDIQSGELIALLGPSGCGKTTL
                     LRIIAGLETPDRGSIHFSGEDTTDVHVRERGVGFVFQHYALFRHMTVFENVAFGLRVK
                     PRGERPSEAQIRDKVMNLLKLVQLDWIAERFPSQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDELHVTSIFVTHDQEEALEVADRVVVINQGRIEQQ
                     GTPQQVWDNPASPFVYGFLGDVNLFKGRANDGRVHLEGGTQLDIPDAQHADGAQAFAY
                     VRPHDLEVERYSPGQAQDAEGRQRGMVVQLARSIVVGPVARLELLPESATESADNVAP
                     EALIEAHISAQQFHTMGLREGETLVVTPRRAKVFLDEAAGI"
     gene            15882..16400
                     /locus_tag="PK518_02940"
     CDS             15882..16400
                     /locus_tag="PK518_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRM47895.1"
                     /translation="MFLNAITGAPRRTLALMCAVCLAMLAFGLYLQYAQGLEPCPMCI
                     VQRYALIGVAACAGLASLRVQKGWWISWSLLALLLAGFGAFTAARQSWLQWYPPEVAT
                     CGRDFYGMVENYPLSRAIPMIFRGSGDCTAIDWTFLGGSIANWSFVWFVLFALVLLAL
                     LLRRQPAGGGLR"
     gene            16415..17401
                     /locus_tag="PK518_02945"
     CDS             16415..17401
                     /locus_tag="PK518_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRM47896.1"
                     /translation="MALPRFAPDHFTLALVGTVVLASLLPASGPMARVLEVATTGVVS
                     LLFFLHGAKLSRQAILGGLGHWRLHLSIFAATFALFPLLGWALRPVLEPLVTPEMYLG
                     VLFLCTLPATVQSAIAFTAMARGNMPAAVCSASASTLLGIVVTPILVGLVLGRSAEGG
                     SVLGAISKISLQLLLPFIVGHLLRPLIGGFIHRHAKRVKLVDQGSILLVVYSAFSAAV
                     VSGLWRETPLPALAGLLVLCAVLLTLVLTLTTWGARALGFAKEDEITLVFCGSKKSLI
                     SGVPMAKVLFASSTVGAIVLPLMIFHQLQLMVCAVLAQRYARRPEHPATQPA"
     gene            complement(17331..18035)
                     /locus_tag="PK518_02950"
     CDS             complement(17331..18035)
                     /locus_tag="PK518_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRM47897.1"
                     /translation="MTALPVLYSFRRCPYAMRARLALVYAGLTCELREVQLRNKPQAL
                     LDASPKGTVPVLVLAGGRVLEQSLDIMLHALRENDPDGWLAPTGARLSDMLALIARND
                     GFFKQALDRCKYPERHGAAAVHQARLDAQAWLSQLNQQVMASGHLFGHKPSLADMALL
                     PFVRQYARIDEGQWTAQPWPHLQGWLQRWLDSALFAEIMQRHPAWAPGMACVTLAGSR
                     DARAGAHSAAPAPRTP"
     gene            18236..19846
                     /locus_tag="PK518_02955"
     CDS             18236..19846
                     /locus_tag="PK518_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRM47898.1"
                     /translation="MPNAMQKLLQRLQLLNEVGAALSRERDIDHLLERILDAAQMLTH
                     ADAGTLYRVTEDGSALRFALMRTHSLGLHQGGSSGQAVEFPDLPLYLPDGRANDSLVA
                     VHAAVHDHTVSIADAYDSTEFNFAGARAFDLSTGCRSRSFLTVPLRNHDRELVGVLQL
                     INSIDPATGAVRAFSQQDRSLAESLASQAAIALSNRLLITQLERLFESFVNLINLAID
                     EKSPYTGGHCERVPALTMMLAEAAHATTDGPLAAFVMTERDRYELKMAGLLHDCGKIT
                     TPVHVVDKATKLQTIFDRIGLVDTRFEVLKRDAEIDMLRRQLALRPQVDAAAEARLQD
                     GFQDQRQQLDEERAFLRRCNLGSEAMRPEDQARVQTIGSAYHWRNAEGWVEDFLSDDE
                     VENLSIRSGTLTRTERGIINHHIVATIKMLESLPWPSHLRNVPEYAGGHHERMDGKGY
                     PRGLQREQLSWQARMLGLADIFEALTAADRPYKQSMTLSQALAVMRKMVQGGHIDPDL
                     FDVFVQGKVYQRYAERFLDPEQIDDVRL"
     assembly_gap    19900..19999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20109..21830
                     /locus_tag="PK518_02960"
     CDS             20109..21830
                     /locus_tag="PK518_02960"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM47899.1"
                     /translation="MISVDQIVDLHLPQLQRHPWLSKGVRGMLRRLLHEQSFRHFAQQ
                     YPHLEGFPFVEQVLEHFAFSYAVRDNERERIPARGRVVIIANHPLGSLDGLALLNLVG
                     SIRSDVKIVANGLLATLAPLQRLLLPVTVLGGHSGARQLKAILEHLRGEGAVIIFPAG
                     EVSRLRPGGVRDGRWHSGFLRLAAQTQAPIVPIHIDGRNSALFYGASLCYKPLATLLL
                     VQEMFGQHRKSIALRIGHPIPHSSHGAMPLPDAAKAKLFCKHLYRVAQDRPGLLHTET
                     AVAHPVDRSALAQAVGACALLGQTPDGKRIHLFRGALDSPVLREVGRLRELAFRAVGE
                     GSGRRRDLDRYDLIYDHLILWDPQDLEIVGAYRMVRTAAVVQDQGVEGLYTHSLFQFG
                     PRMVPYLQQGLELGRSFVQPRYWGRRSLDYLWFGIGAYVRRYPECRYLFGPVSLSRDM
                     PQAARDLLVHHYRSHYGEPGVAEARRPYVPGSDLETVASGDSSADFVRLKHLLAHLGC
                     SVPTLYKQYTEVAEPGGVRFLDFGVDPEFSHCVDGLVLVDVQQLKAQRRERYIGSAGT
                     AGALTAA"
     gene            complement(21817..23163)
                     /gene="argA"
                     /locus_tag="PK518_02965"
     CDS             complement(21817..23163)
                     /gene="argA"
                     /locus_tag="PK518_02965"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HRM47900.1"
                     /translation="MSTVFNFTFVPWFRSVAPYIHKFRHQTFVVGLTGEAIAAGKLQN
                     IVQDLALIQAMGVKIVLVHGFRPQVNEQLAAKGHAARYSLGMRITDPIALDCAQEAAG
                     QLRYEIEAAFSQGLPNTPMAGSRVRVISGNFLTARPVGIVDGVDFQHTGLVRKVDVEG
                     IRRTLASEAMVLISPFGFSPTGEAFNLAMEEVATRVASELKADKLLFLTEVSGIRTKP
                     LEPEGEDNPIDTELPLAAARQLLAQLPPAQQPTDMGFYLQHCIRACEHGVDRSHILPF
                     ARDGALLLEIYVHDGIGTMVVDEKLEEMREATIDDVGGILQLIEPFERDGTLVKRDRT
                     EIERDISAYTVIEHDGVIFGCAALYPYPEANTAEMAAVTVSPNSQGTGDGEKLLKRIE
                     QRARLLGLASIFVLTTRTMHWFIKRGFQPVAPDWLPEARKRKYNWDRKSQVLVKRL"
     gene            complement(23372..24058)
                     /locus_tag="PK518_02970"
     CDS             complement(23372..24058)
                     /locus_tag="PK518_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRM47901.1"
                     /translation="MKHAIKKTGLYVATAIALAFGCASAAQAATVSWTDWTNTSAAAS
                     QVFGDLDINGAHVGVTFTGAYAFAQTTGGTNYWSPAAPYLSSLAGNAPPASDIIALST
                     GGTATITFSQPVVDPLIALVSWNGNTVDFGVPIEVLSYGSGYWGNGTPVLNPTSTGFF
                     GNGEVHGVIRLPGTYSSVTFTHTTENWHGFTVGAIGQGNGNAVPEPGSVALISLALFT
                     LGATLRRRRS"
     gene            complement(24213..24758)
                     /locus_tag="PK518_02975"
     CDS             complement(24213..24758)
                     /locus_tag="PK518_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM47902.1"
                     /translation="MDFADYRMAFEMAPVGLVISRNRAMVDCNRQVCEMFGASRELLI
                     GQSFQILYPSVDEYERLGARMAPILNARGYYADSRIMKRANGETFWCHVSGRALDRQD
                     PHASGIWSFEDLSAQRPVKAELTAREREVAARLLEGMTSKEIGRALDISHRTVEIYRA
                     RLMRKYSASTAADLVQKLLLG"
     gene            24841..25545
                     /locus_tag="PK518_02980"
     CDS             24841..25545
                     /locus_tag="PK518_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRM47903.1"
                     /translation="MKNKLYSSADEALKGVVQDGQLLAVGGFGLCGIPEALIQALHDS
                     GVKNLTAISNNAGVDGFGLGKLLETRQIKKMISSYVGENKEFERQYLAGELELEFTPQ
                     GTLAEKLRAGGAGIPAFFTRTGVGTIVAEGKETREFDGHTYLMERSLVPDVSLVKAAV
                     ADKSGNLRFNLTARNFNPAVAMAGKICIVEVEKIVEVGELAPDDIHLPGIYVHRIVLN
                     AHPEKRIEKRTLTETK"
     gene            25601..26239
                     /locus_tag="PK518_02985"
     CDS             25601..26239
                     /locus_tag="PK518_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRM47904.1"
                     /translation="MAWTQDQMAARAAQELQDGFYVNLGIGIPTLVANHTGDKEVWLQ
                     SENGMLGIGPFPTEDQVDADLINAGKQTVTTIPGTSIFGSHDSFAMIRGGKINLAILG
                     AMQVSEKGDLANWMIPGKMVKGMGGAMDLVGGVPKVIVLMEHVARKKDGSTDMKILPQ
                     CTLPLTGVGVVHIIITDLGVMDVTPQGLKLVELAPGVSFEEIQSKTGVTLLR"
     gene            26380..27738
                     /locus_tag="PK518_02990"
     CDS             26380..27738
                     /locus_tag="PK518_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRM47905.1"
                     /translation="MNVFETSLAASIADDANAPGDNRHYLRTLTDMADRGGVLTHDAI
                     YTDRGVKLVDKGTRVDSELYDRLVKHKLRGPIEDHLSVENMVSVQSLLQVARTQCEGD
                     TLVYLLVHTLPDMTESKLLAPVRAMPLPPALAFKLTVMREQHPLLFAHSVRMMLVAVY
                     LGIKSGLSERECTPLAAAALLHDLGVLHMDPAWHDPANKITGAGRKQLLAHPVTAMLI
                     IREQNIYPRSVEQAVLEHHECMDGSGYPRRLRGEQISRLGQLLLTAEVVAAFFEKYAS
                     EGAAQRLALTLKLNHRKFPADLVACLLPLLQIRAVQGDVPASQDDVQRCITLLTGAFE
                     DWQRCRVILQPVAGQPSPEPACALVEQRLAALQRSLFESGSHPRQLAEMLPHLQGDAQ
                     GLAELALLGQEALWQLQRIIDTTHSHWPQLSASEDAGDIAVVQWRAACTARLVQAPAT
                     QA"
     gene            27796..>28318
                     /gene="imuA"
                     /locus_tag="PK518_02995"
     CDS             27796..>28318
                     /gene="imuA"
                     /locus_tag="PK518_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HRM47906.1"
                     /translation="MGAALPFFIDDLPGVWRGDAWAQAPQRAVDTGHEQLNAQLPGGG
                     WPLAGMTELLLPIHAHCEWRLLAPALARLLADSSGHAVLVAPPLEPFMPALQAAGVPV
                     PRLCCIRPVGSGAAMAAAWACEQALRCRDVRAVLAWLPQVATQSLRRLQLAAAQNQQL
                     LWVLRPEGARAQAS"
BASE COUNT         4701 a         9295 c         9481 g         4741 t
ORIGIN      
        1 tgctgcgggc catgcccggg gtgttctgcg ccggcgagat gctggactgg gaggctccca
       61 cgggcggtta cctgctcacg gcctgctgcg ccaccgggcg cgtggcaggc gcgggggcgg
      121 tccactggct ggcgagcccg gtgccggcgg atcagaactt ggtgcggtag tacagcatgg
      181 gcccgccgcg cctggcacgc agcagcaggc gcgagttgcc ctccagctgc aggcccacgg
      241 cgccaaactc cggcaccagg ccgcgcgtgc gcgagctgga ccactgcacc tccacccggg
      301 tgccgaaggt cggttgatcc tggtgccaga tcatgcccat ggcattgggc atggcatgca
      361 gttgcgatgt gcgggcccag gcatgtatgt ccagttgcac cctgcgatcg agctgtgagc
      421 gccagcgcac gcccaggtca aaggcttgcg ggctcagttc cacgcccctg gcatacgaca
      481 ccatgcccaa tgtcagtccc aggctggacg cctggcgtgg tgtgagccag ccggtgatat
      541 cggtgtagtg aatggcctgc ggcgacatgg ctcccggctg gcgcagtgca tcggacgggt
      601 acagggcgcc aaagtcgggg tagctgtggc cacgcagcgt gatggggggc gatgccgcgc
      661 tggtcagcgg gtcggccacc tgcgccaccg cagccccgac ccagaccaca aggggcaagg
      721 cgccgcaaag ccatgggcgc cggcccaagg ccgatctgcg ggcaaatggt gcaaacatga
      781 gtgcattcat gacgacaccc tggaggacaa agcctgggat cagtatgaat caccggggtg
      841 cgccccccgc cagccaaggc cccaagcccc tgtgggacag ggctgacacg cacgcagccc
      901 atgccgggcc cggtgggcgt ctggcacagg aagagaaaaa gagggggagg gggaagagaa
      961 gttgacgagc cttacccccg gcactggctc cacagttagg gctgcttggt tccccacctg
     1021 acccggttgg ccgcttcacc atgcggggag gcccgtcagc ggcaattgta cgcggaaccg
     1081 gggggcgttt cgggctcgcc aaagggcggc ggcgggccac atagaatcac cccccatgtc
     1141 ctatctcgtg ctcgcccgca aataccgccc caagaccttc tcggagatgg tcgggcagga
     1201 gcatgtggtg caagccctga cgaatgcgct cacgcagcag cggctgcacc atgcctatct
     1261 gttcaccggc acgcgcggcg tgggcaagac cacggtctcg cgcatcctgg ccaagtcgct
     1321 caactgccag gggccggacg gccagggcgg catcacggcc cagccctgcg gcgtgtgccc
     1381 ggcctgccag gacatcgaca gcgggcgctt tcccgactac accgagctgg acgcggcatc
     1441 caaccgcggc gtggacgagg tgcagggcct tttggagcag gccgtctaca agccggtgca
     1501 ggggcgtttc aaggtcttca tgatcgacga ggtgcacatg ctgaccaaca ccgccttcaa
     1561 cgccatgttg aagacgctgg aggagccgcc cgagtacctg aaattcgtgc tggccacgac
     1621 cgacccgcag aaggtgccgg tgacggtgct ctcgcgctgc ctgcagttca acctgcggcc
     1681 gatggcgccc gagacggtgc tggagcattt gacgcgcgtg ctggcggccg aacaggtggc
     1741 cgccgagccc caggcgctgc gcctgctctc gcgtgcggcg cgcggctcca tgcgcgatgc
     1801 gctgagtttg accgaccagg ccatcgcctt cggcagcggc cagctgcagg aggccagcgt
     1861 gcgcctgatg ctgggcagcg tggaccgcag ccatgtgttc cgcctgatcg aggcgctggc
     1921 cgctggcgac ggccgcaccg tggtggagac ggccgacgcc ctgcgcgtca acggcctgtc
     1981 ggccgccagc acgctggagg aaatggctgc cgtgctgcag cgcatggccg tgctgcaggc
     2041 ggtgccgcag atggccgccg ccgtggacgc cagcgacccc gaggcggcag agattgcccg
     2101 cctggccgcc gccctgccgg ccgacgaaac gcagctgctc tacagcatct gcctgcatgg
     2161 ccgtaccgag ctgggcctgg cacccgatga gtacgccgcc ctgaccatgg tgctgctgcg
     2221 cctgctggcc ttcaagcccg ccggtgccgc tggcgccacg gcggaaaaaa aaactctgac
     2281 gccgcctgaa cccgccgcgc cagcaccggt tgccgtcgca gctgatccgc ccgttgcacc
     2341 gctcgcagtg cagccgccca tccatgtggc gcgtgaaccg cagccgccag ccccactggc
     2401 tgcacccgcc gcacccgagc cggccacgcc cgatgatgtg aaccgcgcgg tgaccgaggc
     2461 catgctgcag gatgcggcgg cgcccaacgc cgtgcctgcg gcgctgccgg gcccggctgc
     2521 cgcgcccacg gtgcgcctac ccgtgcgcag ccctgaggat ttaaggcaaa aacagcatcc
     2581 agcgcccgcc aatcaagcgc aaacagcttc tgaaacaata gcaataccgg tgcgctccgc
     2641 acctgagccc gggccgcgcc tgcaggccgg tgcggcagcg ccgccgctgg tgaccaccga
     2701 ggagggtgat gtctggcatg ccaccgtgca gcagctcatc gcgcaagacg ccatcaccgc
     2761 cctggtgcgc gagctggcgc tgcagtcgca gctgatggcg cgcgacgagg gccactggct
     2821 gctgcgcgtt gagcgcgaat cactgaacca gccccaggcg cgcgaacgcc tgcgcgcggc
     2881 gctggaggcc gcgggccatg cgcgccagat cagcgtggag ctgggcgtgg tcagcgacag
     2941 ccccgcgcgc cgcaacgccc aggccgccag cgcacgccag cgcgcggccg aggacatagt
     3001 ccacaacgac ccctatgtgc agcagctggt gcgcgacttc ggcgcgaaaa tcgtgccggg
     3061 cagcatcaag cctgcgtgac acgctcttac tttttttaat caacgaagga aatttccatg
     3121 ttcaacaagg gacagctcgc cggcctcatg aagcaggccc aggccatgca ggacaacctg
     3181 aagaaggccc aggacgaatt ggccagcgtc gaggtcgagg gcgaatccgg cgccggactg
     3241 gtcaaggtct tgatgacctg caagcacgac gtcaagcgcg tgaccataga ccccagcctg
     3301 ctggccgagg acaaggacat gctcgaagac ctggtggccg cggccttcaa cgccgccgtg
     3361 cgcaaggccg aggaaaccag ccaggaaaag atgggcaaga tcaccgccgg catgccaggc
     3421 ctgcccggcg gcatgaagtt ccccttctga cggtgcctgg gcgcaccgcc ccatgtccga
     3481 taccaattcc ctcgacgcac tgatccaggc gctgcggcgc ctgccgggcg tgggcatcaa
     3541 gtcggcgcag cgcatcgcct ttcatctgct gcagcatgac cgcccagcgg cgcagcagct
     3601 gtcgcaggcg ctggcgcagg cctgcgcgca ggtgcgccat tgccagcgct gctacacctt
     3661 caccgagggc gaggtctgcg ccacctgcat ggacgcgacg cgcgacgcca gccgcctgtg
     3721 cgtggtggag acgcccgccg accaggcggc catggagcgc actgccgcct ttcgtggcct
     3781 gtacttcgtg ctcatggggc ggctgtcacc gctggacggc gtggggccgc gcgacatcgg
     3841 cgtgcaaaag ctgctcgagc gcgccggcga cggcgtggtg caggaggtga tcctggccac
     3901 cagcttcacc gccgagggcg aggccacggc ccacgccatt gccgaggcgc tcaggcgccg
     3961 cggcatgcat gtcacgcgcc tggcgcgcgg cgtgccggtg ggcagcgagc tggaatacgt
     4021 ggatctgggc accattgccc atgcgctggc ggatcggcgg tgacataaac gcacaacgca
     4081 caacgcacaa ccgaggcccc aatgtccaac acctctttca ccctggcgta cggctgcgtg
     4141 ctggtggcgg cgctgctgcc catgttttgt gccctcctgg ccaaggccgg ggccatgccc
     4201 aggggcggca accgcgaccc gcgcgcctgg ctggcggcgc agagcggctg gcgtgcccgc
     4261 gccaatgcgg cccaggccaa cggcttcgaa ggcctgccgt tcttcatcgg cgcggtcatc
     4321 atcgcccacc agctgggtgc gccgcaggcg cgcctggatc agctggcctg cgccttcatc
     4381 gtcctgcgcc tggcctatat cgccctgtat gtgggcgaca aggccatggc gcgcagcctg
     4441 gtctggggcc tgggcctggc ggtgaacatc gccatcctgc tgttgggctg gcgctgaggc
     4501 gcggtttcca cgactgggcg taaaatagcg ccatccggct tgcccattca gcgcaagccg
     4561 ctattttttt gatagttgcg caggcatgtg aatggccttg cgcggcattt ttcctcgttt
     4621 tccatgatcg atctccgggg tatcacccag atttaccagg gcccacaagg cccggtcgag
     4681 gccctgcgcg gcatcgacct gcatgtcacg ccgggcgagg tgttcggcat catcgggcgc
     4741 tcgggcgcgg gcaagagctc gctggtgcgc gtcatcaatc tgctgaaccg cccgtcggcc
     4801 ggccaggtca tggtggcggg gcgcgacatg acgcaactga gtgacgccga gctgcgcgcc
     4861 gcgcgccgcg agatcggcat ggtattccag catttcaacc tgctgtcgtc gcgcaccgtg
     4921 tacggcaacg ccgcgctgcc gctggagctg gccggcatgg cgccggacaa gatcaagaag
     4981 cgtgtcgatc cgctgctcga catggtgggc ctggcgcatc tggccgaccg ttacccggcg
     5041 cagatctcag gcgggcaaaa gcagcgcgtg ggcattgcgc gcgcgctggc cagcagtccg
     5101 aaggtgctgc tgtccgacga ggccacgagt gcgctggacc ccgaaaccac gcgctccatc
     5161 ctggatctgc tgcgccaggt gaaccgcgag ctgggcgtga ccgtggtctt gatcacgcac
     5221 cagatgcagg tcatcaagca gatcgccgac cgcgtggcgg tgatcgaagc cggccgcatc
     5281 gtcgagatgg gcccggtgat cgatgtgttc accaagcccc agcaggacat caccaagagc
     5341 ctgatcgacg aaatcgtgcc gcaggagctg ccggccggcg tgctctcgcg cgtgcgcagc
     5401 ctggcggcgc ggcttgaggc gggggccagc ggccagctgc tgcgcctgtc gtacgccggc
     5461 gagcatgcct acgagcccat cctgtcgcac ctgatccgcg agctgggcct cgacctgtcc
     5521 atcctgcacg gccagatcga cgagattcag gagcagacct ttggctcgct ggcggtgtac
     5581 gccagcggcc ccatggcgcg cattggcgcg gccatcgact atctgcgcgc ccagggggtg
     5641 gtggtgcgca ccgtggacgt gagcgcataa ggagcggatg gtgttcgaga atttttccga
     5701 aatgatgctg gagctgtttg ccacctcgct gtgggagacg gtgatcatgg tcggcgcgtc
     5761 gggcatcctc ggcgcgctgg tgggcattcc gctgggggtg tttttgcgcc tcaccgacaa
     5821 gggcggcatc ctgcagaacg ccccggctaa caccgtagtt ggcggcatcg tgaatgccgt
     5881 gcgctccacg cccttcatca tcctgctggt ggccatcatc ccgttcacgc gcctggtcac
     5941 gggcacgtcg atcggcacct gggcggccgt ggtgccgctg acgctggcgg ccgcgccctt
     6001 catcgcgcgt ctggtggaga cggcgctgcg cgaggtggac ggcggcctgg tcgaggccgc
     6061 ccagtccatg ggcgcgacca ccggccagat cgtctggaag gtgctgctgc ccgaggccat
     6121 gccgggcatc gtcgccgggc tgaccatcag ctttgtgagc ctgactggtt attcggccat
     6181 ggccggcgcc gtgggcggcg gcggcctggg cgacctgggc attcgctacg gctaccagcg
     6241 ctttctgccc gacgtgatgc tggccgtggt ggtgatcctg atcgtcttcg tgcaggcaat
     6301 acagagcctg ggcgactggg tggtgcggcg catgtcgcac cgctgatatc tacttctgat
     6361 ttgatagctg ctcacgcttt atagataagc gctggaggcc tatttgctta gtatctgaat
     6421 gacctcgtca tcctccacct gggtgaaatc ctcgtagtac tggcccacgg cctcgaagtc
     6481 ttgcggcgcc tgcaggcatg ccacctcgtc ggccagcggg cgcaccaggg ccagcgcctc
     6541 gtgcgaggcc acgggcacgg cgcagatcag ccgggcaggc ttgcgctggc gcaggctgtg
     6601 cagcgcggcg gccatggtgg ccccggtggc caggccgtcg tccaccacga tgacgatgcg
     6661 cctggccacc tccagcggcg ggcgtatcgg cgtgtactgc gcgcgccgct ggtggatcag
     6721 ctgcagctgc cggcgctttt cggtggcgat gtaatgcgca tcggcgcccg tcgctgcggc
     6781 gtgcggcgac aggtaggtcc agccgctctc atcgaccgag ccgatggcgt attcgggctg
     6841 gtagggcgcg cccagcttgc gcaccagcac caggtccaga tcgccctgca gcgcctgggc
     6901 gatgcattgc gccatgggca cggcgccgcg cggaatggcc agcaccaagg ggttctggcc
     6961 ggcataggcc tgtagccgct cggccaggcg ccgggcggcg tccaggcggt cgcgaaagac
     7021 aatcgggtgg gtggcggcat gcatgccgtg attgtggaac ttactggcct cgccggtatt
     7081 gcgacaggtc tacgataggc ggcccattgc acaagaaagg tttcatcgtg atttccaaac
     7141 gctccctgct tcaatccacc ctggccctgg cgctggccac cggccttggc ggcgccgcca
     7201 tggcacagga caagccgctg aagattggcg tgacggctgg cccgcatgcg cagatctttg
     7261 agcaggtgaa gaaggtggcg gacaaacagg gcctgaagat ccaggtggtg gagttcagcg
     7321 actatgtgca gcccaacgcc gccctggccg cgggcgacct ggacgccaac agctaccaac
     7381 acaagcccta tctggatgcg cagatcaagg atcgcggcta tccgttcgcc gtggcggcca
     7441 acacggtcaa cttccccatc ggcatttact ccaaaaagat caagaaactg gccgatctga
     7501 aggagggcgc gcgcttcggc attcccaacg accctaccaa tggtggccgc gtactgctgc
     7561 tgctccaggc ccagggcctg atcaagctca aggacggcgc gggcctgaag gccacgccgc
     7621 tggacgtggt gggcaacccc aagaagctca agttcatcga gcttgacgcc gcccagctgc
     7681 cgcgctcgct cgacgacctg gacgcctcgg ccatcaacac caactttgcg atctcggccg
     7741 gcctgaaccc caagaccgac gccatcgccc aggagaaccc ggacggcccc tatgtgaacc
     7801 tgctggtggt gcgcgcggcc gacaaggaca agccctgggt ggcgcagctg gtcaaggtct
     7861 accacagccc ggagatccgc cagttcatcg acacccagtt caagggttcg gtgctggccg
     7921 gctggtaatg gctggttgat ggcctgttgt ccgacagcgc aatacgcggc gggctcataa
     7981 gctgcccacg gtcataaccc ccctgactgg agatttctta tgagcaaata ccgcattgcc
     8041 gtcattgtcg gcagcctgcg caaggattcg cacaaccgcc gcctggccca cgccctcacg
     8101 cgcctgggcg cgcccgagtt cgagttccac tacccgcgca tcgacgacct gccgctgtac
     8161 aaccaggacg acgacggcca ccagccagcc ccggtactgc gcctgaagca ggaggtggca
     8221 gagtcccagg gggtgttgtt cgtcacggcc gaatacaacc gctccatccc cggtgtgctc
     8281 aagaacgcca tagacaacgc ctcgcgcccc tacggccaca gcgcctgggc caacaagccc
     8341 gccggggtga taggtgtgtc cataggcacc attggcacgg ccatggcgca gcagcatttg
     8401 cgcaatgtgc tggtgttcct gaacatgccc accatggccc agcccgaggc cttcatccag
     8461 aacagcgacg ccttgttcaa tgccgacggc agcattggcg aggtcagcag ggcgttcttg
     8521 caggcttgga tggatagcta cgtcgcctgg gtcaagcgcc acaacgcctg aacccggggt
     8581 taccaggcgc cgtggtgctc cggcacgcgg gcaaccaggg gcagcgccag caacaacaga
     8641 gcggccacgg ccgcatacac ccaggcctgg cgcatgcccg catccgcggc gcccgccagg
     8701 gccgccgtgg ccagcgacac cacggcgatg gtgcccagct gaaagcacag cgaacgcagc
     8761 ccggcaatgg atgacgactc ctggggtgcc agctgcagcc cggcattgcg gctggccgga
     8821 ttcaccgcgc ccgtgcccag gcccagcacc agggctgcac cggtcagcca ggcatgcggc
     8881 gccatgcccg ccggtggcgc cagtgccacc agcagcatgc ccacggcgct gatggcgccg
     8941 cccacgtaca gcggcagccg atagccggta cgccgcagcg ccatggtggc cagcaccgac
     9001 agcacgatgg aagcgcagcc ctgggccacg agcagcgtgc cggcatacag cgcattcatg
     9061 ccgtagcggt tgcttgcgta cagcgggatc aatgccacgg cgcctatgct gatgccgcca
     9121 tagaccatgt tcaccaggct caccacgcca aagccgggcc catggatcag ccgcgggctg
     9181 atgaagggcg gctcggcgcg cgccacatgg cgcaggaaca gccagccgct gacgccggcc
     9241 acaccggcca gcagggcgag cagggccggc ccgaccggcg catgcgcctc acccaggtag
     9301 ctggccacca gcatgcaggc aaacagcgtc acggccaaca gggccatgcc ggcgccatcc
     9361 atgcgcagcc gctgctgctg cccttggggc gggtcgcgcg gtatgtagcg cagtgccagc
     9421 acggcaacga ccaggcctat gggcacgttc acgaagaaca cgtcgcgcca gctggcgtag
     9481 gtgacgaaca ggccgccaaa aatggggccg atcatggccc ctatgggaaa gatggtgcca
     9541 aacaggccca cggcgcggtc gcgcgcctgg ccgaaatgat cgaccaagat gcccgtggcc
     9601 gatggcgtga aacccgcgcc accggcggcc tgcagcgcgc gcagcagcac cagggtgacg
     9661 atgtcgtcgg ccatgccgca ggcaagggat gcggcggtga acgtgatgac cgagcccagg
     9721 aacacgcgcc ggcgcccgta gcggtcgctg agccggccgc tgatgggcag catgagcacg
     9781 aagccgaagg cgtaggcggt aatgctccag ccggcccagt tcacgctggt gtgcagatcc
     9841 tgctgcagtg catgcagggc ggtggcgacg atggttgagt ccaccgacat catcaacagg
     9901 gccagcgaga cgatgcagaa caccagcacg cgccgtgccg gcgtcagctc ggctgacgcg
     9961 gccggggtgt caggtgctgc gccggtcatc cagccatgct acgcgcaggc ggcaaccagc
    10021 agggcagggg gcttattcag agtggcgcct caggcgactt cggccaggcg ctcgcggttg
    10081 gcggcttcgt ggtgctggac atgcgcggca atggcctgcg cggcggccgc gagcgcctgg
    10141 gccttggcat cggcacccat ggccacgccc tcggcgcgca cgaagcgtac atctgtcacg
    10201 ccaaagaagc cgagcacggt ctgcaggtag ctctcctggt gctccatggc ctggccggta
    10261 tcgctggtgg agtacacgcc gccgcgactg gacgcgatga tcaccgtctt gcccttggcc
    10321 aggccctcgg gaccattggc ggtgtagcgg aaggtgcggc caggctgggc caggcgatcg
    10381 atccaggcct tgagctgcgt gggaatgctg aagttgtaaa acggcgcgcc gatcaccacc
    10441 acatcggccg ccaggaactg gcgcaccagg cgttcggaga cggcgttctc gcgctgctgc
    10501 gcctcgctca ggccctcggt ctggccggtg cgtggggcca tggcgtccat ggtgaagtgg
    10561 gccggggcct cggccaccag atccaggtat tgcacctggg tggcggggtg gctggcctgc
    10621 caggcctcga cgatctgggc cgtgagctgg cgcgaaaagg actggtggcc ggtgatggcg
    10681 gaatcgatgt gcagcagttg catggaatct cctgtgtctg ggttaaccct tggtttgggc
    10741 gttggttgaa ttgtagttcg tcctgaatac ttgataagtg ctgtgtattg cgatagattg
    10801 tcctgaattt gattacaaac accacgatgc aagacctgaa cgacatgctg tatttcgccg
    10861 aggtggccga gcgcggcggc tttgccgcgg cgggccgggc cctgggcata ccgaagtcgc
    10921 gcctgtcgcg ccgcgtggcc gagctggagg cgcaactggg cgtgcgcctg ctgcagcgca
    10981 ccacccgcac cctgtcgctg accgaggtgg gcgaggccta cctgcgccac tgccaggcga
    11041 tgcgcgaatc ggcccaggcc gcggcggacg ccgtggccca ggtgcagtcc gagccgcgcg
    11101 gtacggtgcg cgtgacctgc ccggtgacgc tggcgcagac ggtgctggct cggttgatgc
    11161 ccgtgtttct ggagcgccac ccgcaggtgc ggctggagat gcaggtcacc aaccgcgtgg
    11221 tcaacgtggt ggaggagggt gtggacgtcg ccctgcgcgt gcgcgccacg ctggacaaca
    11281 gcggcagcat gatcgtcaag cgcctggacg aggggcggca gctgctggtg gccagccccg
    11341 cgcaactggc gcggcagggc atgccggcga cgctgcagga tctggccggc atggacacgc
    11401 tggccatgtc ggctaccgac gggcgtacca gcctgcgcct taccggccct ggtggccgcg
    11461 aggagacggt gcagttcacg ccgcgctatg tggccgacga cctgctgacg ctgaaatacg
    11521 ccgccctggc cggcaccggc ctgtgctggc tgccggacta catgtgccag catgagctgc
    11581 agcagggccg gctggtgcag ctgctgcccg aatggtcgca gccccccggc atcgtgcatg
    11641 cggtgttcgc ctcgcgccgg gggctgtcac ccgcagtgcg cggctttctg gactttctgg
    11701 gtgaaacggc aaggggggcc aagggtgacg cgcctcaata gatgaatttt ctataaattc
    11761 acaaccatat attcttttga gttttaagag cctctcccca caatgcctcc cattcctgca
    11821 atccggagag gcacacatga caacccgaat caagttcaag accgttttcg ccaccctggc
    11881 tctggccgcc agcgggctcg catcggctca gacccagtcc ctgctcaacg tctcctacga
    11941 cgtggcgcgc gagttctaca aggactacaa ccaggccttc atcgcccatt acaagaagac
    12001 caagggcgtc gatatcaagg tggatcaggc gcacgcgggc tccagtgccc aggcgcgtgc
    12061 cgtcaacgac ggcctggccg ccgacgtggt gaccttcaac accacgaccg acgtgcagtt
    12121 cctggctgac aacggcgtgg tggcgaagga ctgggcacaa aaattcccgc atgaggcatc
    12181 gcccaccacc tcgaccatgc tgttcctggt gcgccacggc aaccccaaga acatcaagga
    12241 ctgggccgac ctgacccgcc ccgacgtgaa ggtcatcgtc gtcaacccca agaccggcgg
    12301 caatggccgc tacgcctacc tggcggcctg gggcagcgtg cgcgagaagg gtggcagcga
    12361 tgcccaggcg gcagagttca ttacccagat gtacaagaac gtgccggtgc tggccaaggg
    12421 cgggcgcgac gccacggcca ccttcttgca gcgcaacatt ggcgacgtgc tgatcacctt
    12481 cgagtccgaa gtggtgtcgg tggagcgcga gttcggcaag ggcaaggtcg atgccgtcta
    12541 cccctcggtg agcatcaccg ccaagaaccc ggtggcgatc gtggagcgca ccgtcgccaa
    12601 gaagggcacg ggcgaactgg ccaaggccta cctggactac ctgtactccg acgaggcgca
    12661 ggagattgcc gcccgccacg cgattcgccc gcgctccgag gccgtgctca aaaaacatgc
    12721 cgacctgttc aagcctatcc cgcagtttgc cgtggccaag tacttcggct cgctggtcga
    12781 ggcgcagaag gtgcacttca acgacggcgg ccagttcgac aagctgtacc aacccggcaa
    12841 ataagggcgg tgccgcggtg aattcctcca ttgccttgcc cggtgtcgcc atgggcgcgg
    12901 ccagtgacct gaccggtgcg gccgcacccc ggcgccgtcc taagcgcgtg ctgcccggtt
    12961 ttggcctgac gctgggctac acgctgttct acctgagcat catcgtgctc atcccgctgc
    13021 tggcgctgct ggccaagagc ttttcgctga gctggccgca gttctgggag gccgtcagcg
    13081 cgccgcgcgt gctggcctct tacaagctga cgttcggcgc gtccttcatc gccgcctgcg
    13141 tgaatctggt gtttggcctg ctgattgcct gggtgctggt gcgctaccag ttcccgggca
    13201 agaagatcgt cgatgccttt gtggatctgc cgttcgccct gcccaccgcc gtggccggca
    13261 tctcgctgac cgcgctgctg gcgggcaatg gctgggtggg gcagtacttc gagccctttg
    13321 gcattcagct ggccttcaac cccaacggcg tggtgatcgc gctgatcttc atcggcctgc
    13381 ccttcgtggt gcgcacggtg cagccggtgc tcgaagacag cgagaaggag ctggaagagg
    13441 cggccaccag cctcggcgcc acgcgctggc aggtgttcac caaggtgatc ctgcccagca
    13501 tcacgcccgc gctgctgacc ggctttgcca tggcctttgc gcgtgccatc ggcgaatacg
    13561 gttcggtgat cttcatcgcc ggcaacatgc ccatggtgtc cgagatcacg ccgctgatca
    13621 tcatcggcaa gctcgagcaa tacgacaccg ctggtgccac cgccgtggcc gtggtgatgc
    13681 tgttcttctc cttcatcatg ctgctggtca tcaatgcgct acaggcctgg cagcgacgcc
    13741 acgcggggat gccggcatga gcgccgttgc caacccatcc acacgccgcg cccaggccgg
    13801 caccaccgag gcgccctggg tgcgcttcac gctgatcggc ctggccctgg cttttctggc
    13861 gctgtttttg ctgctgccgc tggccgccgt cttcaccgag gcgctgcgca agggctggga
    13921 tgcctacctg gccggcctgg ccgagcccga tgcctggtcg gccatccggc tcacgctgct
    13981 gacggccgtg atcgccgtgc cgctgaacct ggtgttcggc gtggcggcgg cctggtgcat
    14041 tgccaagtac gagttcaagg gcaaggcctt tctgaccacg ctgatcgacc tgccgttctc
    14101 catctcgccg gtggtcgccg gcctgatgta tgtgctggtg ttcggcgcca atggctggtt
    14161 cggcagctgg ctggcggagc atgacatcaa gatcatcttt gccgtgccgg gcatcgtgct
    14221 ggccaccgtg ttcgtcacct tcccgttcgt ggcgcgcgag ctgatcccgc tgatgcaggc
    14281 ccagggcaac gacgaggagc aggccgccat cgtgctggga gccagcggct ggcagacctt
    14341 ctggcatgtg acgctgccca acatcaaatg gggactggtg tatggcgtga tcctgtgcaa
    14401 cgcgcgcgcc atgggcgagt tcggcgcggt gtcggtggtt tcgggccata tccgcggcca
    14461 gaccaacacc attccgctgc atgtggagat tctctacaac gagtaccagt cggtcgcggc
    14521 ctttgccgcc gcatcgctac tggccctgct ggcgctggtg accctggtca tcaagtcgat
    14581 cgccgaatgg cagggcgagc agcaggccaa ggccgccgcc gccctgccgc ccgagcggcc
    14641 aagtgcctga ttggcctgca gggcatatgt ataaagcgcc aatagctatc aaatcatgag
    14701 tatagaaata cgcaacgtca gcaagcagtt cggcaacttc cacgcgctgc gcgacgtgaa
    14761 cctggacatc cagtccggcg agctgatcgc gctgctgggc ccctccggct gcggcaagac
    14821 cacgctcttg cgcatcattg ccgggctgga gacgccggat cgcggcagca tccacttcag
    14881 cggcgaggac accaccgacg tgcatgtgcg cgagcgcggc gtgggctttg tgttccagca
    14941 ctacgcgctg tttcgccaca tgacggtgtt cgagaacgtg gccttcggcc tgcgcgtcaa
    15001 gccgcgcggc gagcggccga gcgaggcgca gatcagggac aaggtgatga acctgctcaa
    15061 gctggtgcag ctggactgga tcgccgagcg cttcccctcg cagctctcgg gcggccagcg
    15121 tcagcgcatc gccctggcgc gcgccctggc cgtcgagccc aaggtgctgc tgctggacga
    15181 gcccttcggc gcgctggacg ccaaggtgcg caaggaactg cgccgctggc tgcgccgcct
    15241 gcacgacgag ctgcatgtga ccagcatctt cgtcacgcac gaccaggagg aggcgttgga
    15301 ggtggccgac cgcgtggtcg tcatcaacca gggcaggatc gagcagcagg gcacgccgca
    15361 gcaggtctgg gacaacccgg ccagcccttt cgtgtatggg tttctgggtg acgtgaacct
    15421 gttcaagggc cgcgccaacg acggccgcgt gcacctggaa gggggcacgc agctggacat
    15481 tcccgacgcc cagcatgccg atggcgccca ggcctttgcc tatgtgcgcc cgcacgacct
    15541 ggaggtcgag cgctattcgc ccggccaggc ccaggacgcc gaggggcgcc agcgcggcat
    15601 ggtggtgcag ctggcgcgct ccatcgtcgt tgggcccgtc gcgcggctgg aactattgcc
    15661 cgaaagcgct accgaatccg ccgacaatgt ggcccccgag gcgctcatcg aggcgcatat
    15721 ctcggcgcag cagttccaca ccatggggct gcgcgagggc gagacgctgg tggtgacacc
    15781 acgccgcgcc aaggtgtttt tggacgaagc cgccggcatt tgatgcatcg cccgcccttg
    15841 ccgggtggtg gtcaacaccg gccctgcaag aaagagaaag agtgtttctg aacgcgatca
    15901 ccggcgcccc gcgccgcacc ctggcgctga tgtgcgccgt ctgcctggcc atgctggcct
    15961 ttggcctgta cctgcaatat gcccagggcc tggagccatg cccgatgtgc atcgtccagc
    16021 gttatgctct tattggtgta gctgcttgcg caggactggc aagccttaga gtccaaaaag
    16081 gctggtggat tagctggagc ttgctggccc tgctgctggc cggctttggc gccttcaccg
    16141 ccgcccgcca gagctggctg cagtggtacc cgcccgaggt ggcgacctgc gggcgcgact
    16201 tctatggcat ggtcgagaac taccccctga gccgcgccat ccccatgatc ttccgcggtt
    16261 cgggcgactg cacggccatc gactggacct tcctgggcgg ctccatcgcc aactggtcgt
    16321 ttgtgtggtt cgtgctgttt gcgctggtgc tgctggcgct gctgctgcgc aggcagccgg
    16381 ccggcggcgg cctgcgctga caaaggcttg acccatggcc ctgccgcgct tcgcccccga
    16441 ccatttcacg ctggccctgg tgggcacggt ggttctggcc agcctgctgc cggcatccgg
    16501 ccccatggcc cgggtgctgg aggtggcgac caccggcgtg gtcagcctgc tgttctttct
    16561 gcacggcgcc aagctgtcgc gccaggccat cctgggcggc ctgggccatt ggcggctgca
    16621 cctgagcatc tttgccgcca cctttgcgct gttcccgctg ctgggctggg ctctgcgccc
    16681 ggtgctggag ccgctggtca cgcccgagat gtacctgggc gtgctgtttt tgtgcaccct
    16741 gccggccacg gtgcagtcgg cgattgcctt cacggccatg gcgcgcggca acatgccggc
    16801 ggcggtgtgc agcgcatcag cctccacgct gctgggcatc gtggtcacgc ccatcctggt
    16861 ggggctggtg ctggggcgca gcgccgaggg cggcagcgtg ctcggggcca tctccaagat
    16921 ctcgttgcag ctgctgctgc ccttcatcgt cggccatttg ctgcgtccgc tgatcggcgg
    16981 cttcatccac cgccacgcca agcgcgtcaa gctggtcgat cagggctcca tcctgctggt
    17041 ggtgtattcg gccttcagcg cggccgtggt cagcggcctg tggcgcgaga cgccgctgcc
    17101 cgcgctggcc ggcctgctgg tgctttgcgc ggtgctgctg accctggtgc tgacgctgac
    17161 cacctggggc gcgcgcgcgc tgggctttgc caaggaagac gagatcacgc tggtgttctg
    17221 cggctccaag aagagcctga tcagcggcgt gcccatggcc aaggtgctgt ttgcctccag
    17281 caccgtgggc gccatcgtgc tgccgctgat gatcttccac caactgcagc tcatggtgtg
    17341 cgcggtgctg gcgcagcgct atgcgcgccg gcccgagcat cccgcgaccc agccagcgtg
    17401 acgcaggcca tgcccggcgc ccaggccggg tggcgctgca tgatctcggc aaacagggcc
    17461 gaatccagcc agcgttgcag ccagccctgc agatgcggcc atggctgggc ggtccattgc
    17521 ccctcgtcga tgcgcgcgta ctggcgcaca aagggcagca gcgccatgtc ggccaggctg
    17581 ggtttgtggc caaaaagatg gccgctggcc atcacctgtt gattgagctg cgatagccag
    17641 gcctgcgcgt caagccgcgc ctggtgcacg gctgcggcgc cgtggcgctc ggggtatttg
    17701 cagcggtcga gcgcctgctt gaagaaaccg tcattgcgcg caatcagtgc cagcatgtct
    17761 gacagccggg cgccggtggg cgccagccag ccgtcggggt cgttctcgcg cagcgcatgc
    17821 agcatgatgt ccaggctttg ttccagcacc cggccgccgg ccaacaccag caccggcacc
    17881 gtgcctttgg gcgaggcgtc cagcagcgcc tggggcttgt tgcgcagctg tacctcgcgc
    17941 agttcgcagg tcaggccggc atataccagg gccaggcgcg cacgcatggc gtaggggcag
    18001 cggcgaaacg aatacaacac gggcagggca gtcatggctg ggatgaaaca tgtagttgcc
    18061 tccagtatgc ggcgcggcgc gggagatgca gtcctgccca ggggcttgat ccatatcgca
    18121 ggttgttgtt tcttctgcat acatgtgtaa acaaataata cgacaatccg ccccggatca
    18181 ccccagcccg gggaggtcag gcatcccatt tttaggtgaa ccaaggggaa cctgcatgcc
    18241 caatgcaatg cagaaactgc tgcagcgcct gcaactgctc aacgaggttg gggcagcgtt
    18301 gtcacgcgag cgtgatatcg accatctgct ggaacgcatc ctggatgcgg cgcagatgct
    18361 cacccatgcc gatgccggca cgctgtaccg cgtcaccgag gacggcagtg cgctgcgctt
    18421 tgcgctgatg cgcacgcatt cgcttggttt gcaccagggc ggcagctccg ggcaggcggt
    18481 ggaatttccc gacctgccgc tgtatctgcc tgatgggcga gcgaatgatt cactggtggc
    18541 cgtgcatgcg gccgtgcacg accataccgt gagcattgcc gatgcctatg acagtaccga
    18601 attcaacttt gccggcgccc gggcctttga cctgagcacg ggctgccgct cgcgttcctt
    18661 tctcaccgtg ccgctgcgca accacgatcg tgagctggtc ggcgtgctgc agctgatcaa
    18721 ttccatagac ccggccacgg gcgccgtgcg cgctttttcg cagcaagacc gcagcctggc
    18781 cgagtcgctg gcctcgcagg cggcgatcgc gctgagcaat cggctcttga tcacgcagct
    18841 ggagcgcctg ttcgagtcct tcgtgaacct catcaacctg gccatcgacg aaaagtcacc
    18901 ctacaccggc ggccactgcg agcgcgtgcc cgcactgacc atgatgctgg ccgaggcggc
    18961 gcatgccacc accgacgggc cgctggccgc ctttgtcatg accgagcgcg accgctacga
    19021 gctgaagatg gccggcctgc tgcacgactg cggcaagatc accacgccgg tgcatgtggt
    19081 ggacaaggcg accaagctgc agaccatttt cgaccgcatc gggctggtcg atacgcgctt
    19141 tgaggtactc aagcgcgatg ccgagatcga catgctgcgc cgccagctcg ccctgcgccc
    19201 gcaggtggac gccgccgccg aggcgcgcct gcaggatggg tttcaagacc agcggcagca
    19261 gctggacgag gaacgcgcat tcctgcgccg ctgcaacctg ggcagcgagg ccatgcggcc
    19321 cgaggaccag gcgcgcgtgc agaccatagg cagcgcgtac cactggcgca acgccgaggg
    19381 ctgggtggag gattttctgt ccgacgacga ggtggagaac ctgagcatcc gctcgggcac
    19441 gctgacgcgc accgagcgcg gcatcatcaa ccaccacatc gtggccacga tcaagatgct
    19501 ggagagcctg ccctggccca gccacctgcg caacgtgccc gagtacgccg gtggccacca
    19561 tgagcgcatg gatggcaagg gctacccgcg cgggctccag cgcgagcagc tgtcgtggca
    19621 ggcgcgcatg ctgggcctgg ccgacatctt cgaggcgctg accgctgccg atcgtcccta
    19681 caagcagtcc atgacgctgt cacaggcgtt ggcggtgatg cgcaagatgg tgcagggtgg
    19741 tcacatcgac cccgatctgt tcgatgtctt cgtgcagggc aaggtgtacc agcgctacgc
    19801 cgagcgcttc ctcgaccccg agcagatcga cgacgtgcgg ctctgacgcg ctgcgggcgc
    19861 ggcgcgcgcc tgtcgcgtat cggtcacggg cctgtcgcgn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnng cctgtcgcgt atcggtcacg ggcctgtcgc gcgcccgtca
    20041 tggcatggcc tcagcatggc tgcagtgccg gagaccccct ccggcgcatg cacctgggag
    20101 tgagcgccat gatctccgtg gaccagatcg tcgacctgca cctgccgcaa ctgcagcggc
    20161 atccgtggct gagcaagggc gtgcgcggca tgctgcgccg cctgctgcac gagcagagct
    20221 tccgccattt cgcccagcag tacccgcacc tggagggctt tccgttcgtc gagcaggtgc
    20281 tggagcattt cgccttcagc tacgcggtgc gcgacaacga gcgcgagcgc attccggcgc
    20341 gcgggcgcgt ggtcatcatt gccaaccacc ccctgggctc gctcgacggg ctggcgctgc
    20401 tcaacctggt gggcagcatc cgcagcgacg tgaagatcgt cgccaacggc ctgctcgcca
    20461 cgctcgcgcc cctccagcgc ctgctgctgc cggtcacggt gctgggcgga cacagcggcg
    20521 cgcggcagct caaggccatc ctggagcacc tgcgcggcga gggggcagtc atcatcttcc
    20581 cggccggcga ggtctcgcgc ctgcgcccgg gcggcgtgcg cgacgggcgc tggcacagcg
    20641 gctttctgcg gctggcggcg cagacgcagg cgcccatcgt gcccatccac atcgacggcc
    20701 gcaactcggc cctgttctat ggcgcctccc tgtgctacaa gccgctggcc acgctgctgc
    20761 tggtgcagga gatgttcggc cagcaccgca agagcatagc cctgcgcatc ggccacccca
    20821 tcccgcacag cagccacggc gccatgccct tgccggacgc ggccaaggcc aagctgttct
    20881 gcaagcacct gtaccgtgtg gcgcaggaca ggcccggcct gctgcacacc gagacggctg
    20941 tcgcccatcc ggtggaccgc agtgcgctgg cccaggccgt gggcgcctgc gcgctgctgg
    21001 ggcagacgcc ggacggcaag cgcatccacc tgttccgcgg cgcgctggac tcgcccgtgc
    21061 tgcgcgaagt ggggcggctg cgcgagctcg ccttccgggc cgtcggcgag ggcagcggcc
    21121 gccgccgcga cctggaccgc tacgacctga tctacgacca cctcatcctg tgggatcccc
    21181 aggatctgga gatcgtcggc gcctaccgca tggtgcgcac ggccgcggtg gtgcaggacc
    21241 agggcgtaga ggggctgtac acgcacagcc tgttccagtt cggcccgcgc atggtgccgt
    21301 acctgcagca ggggctggag ctgggccgct ccttcgtgca gccgcgctac tggggccgcc
    21361 gctcgctcga ctacctgtgg ttcggcatcg gcgcctacgt gcggcgctat cccgagtgcc
    21421 gctacctgtt cggcccggtc tcgctctcgc gcgacatgcc ccaggcggcg cgcgatctgc
    21481 tggtgcacca ctaccgcagc cactatggcg agcccggcgt ggccgaggcc cgccgcccct
    21541 acgtgccggg cagcgatctg gagacggtgg cgagcggcga cagcagcgcg gatttcgtgc
    21601 gcctcaagca ccttctggcg cacctgggct gcagcgtgcc cacgctgtac aagcagtaca
    21661 cggaagtggc cgagccgggc ggcgtgcgct tcctggactt cggcgtggac ccggaattca
    21721 gccattgcgt cgatggcctg gtgctggtgg acgtgcagca gctcaaggcc cagcggcgcg
    21781 agcgctacat cgggtccgca ggcacggcgg gcgcgctcac agccgcttga ccagcacctg
    21841 gctcttgcga tcccagttgt acttgcgctt gcgcgcctcg ggcagccagt cgggcgccac
    21901 gggctggaag ccccgcttga tgaaccagtg catggtgcgc gtggtcagca cgaagatgct
    21961 ggccaggccc agcaggcgcg cgcgctgctc tatgcgcttg agcagctttt caccgtcacc
    22021 cgtgccctgg ctgttgggcg agacggtgac ggcggccatt tcggcggtgt tcgcctcggg
    22081 gtaggggtag agcgcggcgc agccaaagat cacgccgtca tgttcgatca cggtgtaagc
    22141 cgagatatcg cgctcgatct cggtgcggtc gcgcttgacc agggtgccgt cgcgctcgaa
    22201 gggctcgatc aattgcagga tgccgcccac gtcgtcaatc gtggcctcgc gcatttcctc
    22261 cagcttttca tcgacgacca tggtgccgat gccgtcgtgc acgtagatct ccagcagcag
    22321 ggcgccgtcg cgcgcgaagg gcaggatatg gctgcgatcc acgccatgct cgcaggcgcg
    22381 tatgcagtgc tgcaggtaga accccatgtc ggtgggctgc tgcgccgggg gcagctgcgc
    22441 cagcagctgg cgcgcggcgg ccaggggcag ctcggtgtct atggggttgt cctcgccctc
    22501 gggctccagg ggcttggtgc gtatgccgct cacctcggtc aaaaacagca gcttgtcggc
    22561 cttgagctcg ctggccacgc gcgtggccac ctcctccatg gccaggttga aggcctcgcc
    22621 cgtgggcgag aagccaaacg gcgagatgag caccatggcc tcggaggcca gcgtgcggcg
    22681 tatgccttcc acgtccacct tgcgcaccag gccggtgtgc tggaagtcca ccccatcgac
    22741 gatgcccacc ggccgtgcgg tcaggaagtt gcccgagatc acacgcacgc gcgagcccgc
    22801 catgggggtg ttgggcaggc cctggctgaa ggcggcctcg atttcgtagc gcagctggcc
    22861 cgcggcctcc tgggcgcagt ccagcgcgat cggatcggtg atgcgcatgc cgagcgagta
    22921 gcgtgcggca tgccccttgg ccgccagctg ttcgttgacc tgcgggcgaa agccgtgcac
    22981 cagcacgatc ttcacgccca tggcctggat caaggccaga tcctgcacga tgttctgcag
    23041 cttgcccgcg gcaatggctt cgcccgtcag ccccaccaca aaggtctggt gccggaactt
    23101 gtggatatac ggcgccacgg agcggaacca gggaacgaag gtgaagttga agaccgtgga
    23161 catggtggct tgggcgtggg cgggcgagca aaggcaaaaa agcctgcgaa ccccaggcca
    23221 cccaggcggt ggttacggcg tcggcaagcg aagcgcgcag tttagcggct gcgtgggcgc
    23281 cttgtgtgcg cgtgcggcgt ccaggatctg cccggcagtg tggagcggcg cagatacgcg
    23341 accgacgtcg tgttgaaggc gttgtcgtga ctcagctgcg acgcctgcgc agggtggcgc
    23401 cgagtgtgaa aagcgccaag gagatgagag ctaccgatcc cggttctgga accgcgttgc
    23461 cgttaccttg gccgatcgcg cccacagtga atccatgcca gttctcggtg gtatgtgtga
    23521 atgtcaccga gctataggtg cccggcagac ggatcacgcc gtgaacttca ccgttgccga
    23581 agaagccggt gctggtcggg ttaaggactg gagtcccgtt gccccaatag ccggatccgt
    23641 agctcaaaac ctcgatcggc acgccgaagt cgacggtgtt gccgttccag ctcacgagcg
    23701 caatcagcgg atcgacgaca ggctgtgaaa aggtgatcgt ggctgtcccg ccggtgctca
    23761 gtgcgatgat gtccgatgcg ggtggtgcgt tgccagccag gctgctgaga tatggcgctg
    23821 ccggcgacca gtaattggtg ccccccgttg tctgtgcgaa cgcataggcg ccggtaaagg
    23881 tgacgccgac atgcgcaccg ttgatgtcga ggtcgccaaa aacctggctt gcagcggcac
    23941 tcgtgtttgt ccaatcagtc caggaaaccg tcgctgcctg ggcggcgctt gcacaaccga
    24001 acgccaatgc tatcgctgtt gcaacgtaga ggcctgtctt tttgattgcg tgcttcatgg
    24061 ttcattccta gtggccggtg atagctgcaa ccaataaagc aatgatcggg ccagaaattc
    24121 aagttattga ttctgcttgg acgacggctc caagcgcatg tcgtgtgtca aatttttcga
    24181 cagtttgatt cgggggatca gaacggcacg agttacccaa gcagcagctt ttgcaccaga
    24241 tctgcggccg tggaggcgct gtacttgcgc atcagccggg cgcggtagat ctccaccgta
    24301 cggtggctga tgtccagggc gcggccgatt tccttggagg tcatgccctc cagcaggcgc
    24361 gcggccacct cgcgctcgcg cgccgtgagc tcggccttca cggggcgctg ggcgctgagg
    24421 tcttcaaagc tccagatgcc cgaggcatgc ggatcctggc gatcgagggc gcggcccgag
    24481 acatggcacc agaaggtctc gccattggcg cgtttcatga tgcggctgtc ggcgtagtag
    24541 ccccgggcgt tgagtatcgg ggccatgcgc gcgcccaggc gctcgtattc atcgacgctg
    24601 gggtagagaa tctggaacga ctggccgatg agcagttcgc gcgaggcgcc aaacatctcg
    24661 cagacctggc ggttgcagtc caccatggcg cggttgcgcg agatcaccag gcccacgggc
    24721 gccatctcga aagccatgcg gtaatcggca aagtccatgg tggtgcaagt ctttagggaa
    24781 tactacgtaa tcgaatacgt agtatcgtag cgcattcccg tagtacttca ggagacagta
    24841 gtgaagaaca agctttattc atcggcggac gaggcgctca agggcgtcgt gcaggacggc
    24901 cagttgctgg ccgtcggtgg cttcggcctg tgcggcatcc cggaggccct gatccaggcc
    24961 ctgcacgaca gcggtgtgaa gaacctcacc gccatttcca acaacgccgg cgtggatggc
    25021 tttggcctgg gcaagctgct ggagacgcgc cagatcaaga agatgatcag ctcctacgtg
    25081 ggcgagaaca aggaattcga gcgccagtac ctggccggcg aactggaact ggaattcacc
    25141 ccccagggca cgctggccga gaagctgcgt gccggcggcg ccggcattcc ggccttcttc
    25201 acgcgcaccg gcgtgggcac catcgttgcc gagggcaagg aaacccgtga gttcgacggc
    25261 catacctacc tgatggagcg ctcgctggta cccgacgtgt cgctggtcaa ggcggcggtg
    25321 gccgacaaga gcggcaacct gcgcttcaac ctgacggcgc gcaacttcaa cccggccgtg
    25381 gccatggccg gcaagatctg catcgtcgag gtggagaaga tcgtcgaggt gggcgagctg
    25441 gcgcccgacg atatccatct gcccggcatc tatgtgcacc gcatcgtgct caatgcccat
    25501 cccgaaaagc gcatcgagaa gcgcaccctg accgaaacca aataatttgt ggggcggatt
    25561 tgccaatccc tgccccatgc catcaatagg agagacagac atggcctgga ctcaagacca
    25621 gatggccgca cgcgcggccc aggaactgca agacggcttc tacgtcaacc tcggcatcgg
    25681 cattcccacg ctggtggcca accatacggg cgacaaggag gtgtggctgc agtccgagaa
    25741 cggcatgctg ggcataggcc cgttcccaac cgaggatcag gtggatgccg acctgatcaa
    25801 cgccggcaag cagaccgtga ccaccatccc cggcacgtcg atcttcggta gccacgacag
    25861 ctttgccatg atccgcggtg gcaagatcaa cctggccatc ctaggtgcca tgcaggtcag
    25921 cgagaagggc gacctggcca actggatgat tcccggcaag atggtcaagg gcatgggcgg
    25981 agccatggat ctggtgggtg gcgtgcccaa ggtcatcgtg ctgatggaac atgtggcgcg
    26041 caagaaggac ggcagcaccg acatgaagat cctgccccag tgcacgctgc cgctgacggg
    26101 cgtgggcgtg gtgcacatca tcatcaccga cctgggcgtg atggatgtca cgccccaggg
    26161 cctgaaactc gtggagctgg ccccgggtgt gagcttcgag gaaatccagt cgaagacggg
    26221 cgtgacgctg ttgcgctaag ggcttgccgc tcctgagctg gatcggtgca ttttccccat
    26281 acatcaggcc ggaggtgaca ctccggcctt tgtttttttg ctatggcgcg caaagcaccg
    26341 tatagtcgcc ggcgacattt agaaacacat gatccgtgca tgaacgtttt cgaaaccagt
    26401 cttgcggcct cgatcgccga tgacgcgaac gcccccggtg acaaccgcca ttacctgcgc
    26461 accctcaccg acatggccga ccgcggcggc gtgctcacgc acgatgccat ctacaccgac
    26521 cgtggcgtca agctggtgga caagggcacg cgcgtggaca gcgagctcta cgatcgcctg
    26581 gtcaagcaca agctgcgcgg ccctatcgaa gaccacctga gcgtggagaa catggtcagc
    26641 gtgcagtcgc tgctgcaggt ggcgcgcacg caatgcgaag gcgacaccct ggtctacctg
    26701 ctggtgcata cgttgccaga catgacggag agcaaactgc tcgccccggt gcgcgccatg
    26761 ccgctgccgc cggcgctggc cttcaagctc acggtgatgc gggagcagca ccccctgctg
    26821 tttgcgcaca gcgtgcgcat gatgctggtg gccgtgtatc tgggcatcaa aagcggcctg
    26881 agcgagcgcg aatgcacgcc cctggccgcg gcggcgctgc tgcacgacct gggggtgctg
    26941 cacatggatc cggcctggca tgacccggcg aacaagatca ccggggcagg gcgcaagcag
    27001 ctcttggcgc atccggtcac ggccatgctc atcatcagag agcaaaacat ctatccgcgc
    27061 tcggtggagc aggcggtgct ggagcaccac gaatgcatgg acggcagcgg ctacccgcgg
    27121 cgcctgcgcg gcgagcagat ctcgcgcctg gggcagctcc tgctgaccgc cgaggtggtg
    27181 gccgccttct ttgaaaaata tgccagcgag ggtgcggccc agcgcctggc gcttacgctc
    27241 aagctgaacc atcgcaagtt tccggccgat ctggtggcct gcctgctgcc tttgctgcag
    27301 atacgggccg tgcagggtga tgtgccagcc tcgcaggacg atgtgcagcg ctgcatcacg
    27361 ctgttgaccg gggctttcga ggattggcag cgctgccgcg tcatcctgca gcccgtggcc
    27421 gggcagcctt cccccgagcc ggcctgtgcg ctggtcgagc agcggctggc ggcgctgcaa
    27481 agatcgctgt tcgagtctgg ctcccacccg cggcagctgg ccgagatgct gccgcacctg
    27541 cagggcgacg cccagggcct ggccgagctg gccctgctgg ggcaagaggc gctgtggcag
    27601 ctgcagcgca tcatcgacac cacccacagc cattggccgc aactgtcggc cagcgaggat
    27661 gccggcgaca ttgccgtcgt acaatggcgc gctgcctgca ccgccaggct ggtgcaagcg
    27721 ccggccacgc aggcctgatt tgggcatgtc tcgcatgaat actgtatatt taaacagtat
    27781 tcatgcgagg tacccatggg cgctgctctt ccttttttca tagacgattt acccggtgtc
    27841 tggcgcggtg acgcctgggc gcaggcgcca cagcgggcgg tggacacggg ccacgagcag
    27901 ctgaatgcac aactgcccgg gggtggctgg cccttggcag gcatgaccga gttgttgctg
    27961 cccatccatg cccattgcga atggaggctg cttgcgcccg cgctggcgcg tctgcttgca
    28021 gactcgtccg gccatgccgt gctggtggcg ccgccgctgg agcccttcat gcccgcgttg
    28081 caggccgctg gcgtgcccgt gccgcggctg tgttgcatca ggcctgtggg ctccggtgcc
    28141 gccatggccg ccgcctgggc ctgcgagcag gcgctgcgct gccgcgacgt gcgcgccgtg
    28201 ctggcctggc tgccgcaagt ggcaacgcag tcgctgcgcc ggctgcagct ggcagccgcg
    28261 cagaaccagc agcttttgtg ggtgctgcgg cccgagggcg cgcgagccca ggcgtcgc
//
LOCUS       DAOMTQ010000016        28187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_152_length_28187_cov_27.465413, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000016 DAOMTQ010000000
VERSION     DAOMTQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 28187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28187
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152_length_28187_cov_27.465413"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PK518_03000"
     CDS             <1..524
                     /locus_tag="PK518_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM47907.1"
                     /translation="LGDKHVFFWLLTNRMTPPAVFLLPFFQLYTTVGLMDTHIAVALA
                     HLLFNVPLAVWILEGFMSGIPREIDETAYIDGYSFPRFFLTIFLPLIKAGVGVAAFFC
                     FMFSWVELLLARTLTSVNAKPIVATMTRTVSASGMDWATLAAAGVLTIVPGAIVIWFV
                     RHYIAKGFAMGRV"
     gene            526..834
                     /locus_tag="PK518_03005"
     CDS             526..834
                     /locus_tag="PK518_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HRM47908.1"
                     /translation="MFEWMAWTTPVAVFFTCIVLMLIGMTVWEIRSPTVERKGFLPMA
                     TTRGDRLFIGLLTAAYINLAWVGLGEKMAQWFSLEAEPSVWISFVLSMLVLVLVLRKG
                     "
     gene            1013..2746
                     /locus_tag="PK518_03010"
     CDS             1013..2746
                     /locus_tag="PK518_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM47909.1"
                     /translation="MKMQFTAIAFATAAMALGQAAWAGEAEAKKWIDSEFQPSTLSKD
                     QQMAEMKWFIEAAKKLQAKGVKEISVVSETITTHEYESKTLAKAFSEITGIQVKHDLI
                     QEGDVVEKLQTSMQSGKSIYDGWISDSDLIGTHYRYGKILNLTDYMAGAGKDYTNPGL
                     DLKDFIGTKFTTAPDGKLYQLPDQQFANLYWFRADLFDRKDLKDKFKAKYGYDLGVPL
                     NWSAYEDIAEFFTNDVKQIDGKPIYGHMDYGKKDPSLGWRFTDAWLSMAGSADIGTPN
                     GLPIDEWGIRVADDKCTPVGASVARGGATNSPAAVYALTKYVDWMKKYAPKEAMGMTF
                     GEAGPVPAQGQIAQQIFWYTAFTADMNKPGLPVVNADGTPKWRMAPGPNGPYWKQGMQ
                     NGYQDVGSWTFFKDHNPDRLAAAWLYAQFVTAKTTSLKKTVVGLTPIRESDIRSKAMT
                     DMAPKLGGLVEFYRSPARVAWSPTGTNVPDYPKLAQLWWKNVAQAVTGEKTPQAAMDT
                     LADEMDQVMARLERAGMERCAPKLNKKEDPKKWLSDKHAPWAKLANEKPKGETINYNT
                     LLQAWKDGKVR"
     gene            2848..4443
                     /locus_tag="PK518_03015"
     CDS             2848..4443
                     /locus_tag="PK518_03015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRM47910.1"
                     /translation="MTSDTSTPLATVRADLLARLAQPVTYDLAVVGGGATGLGVALDA
                     AARGFSVVLLESHDFAKGTSSRATKLVHGGVRYLAQGNIALVREALHERTTLLHNAPH
                     LAQPLAFVMPSYKLWETPFYGVGLKMYDALAGQAGLGSTQFLGRMETLRCLPTARADG
                     LKGGVKYWDGQFDDARLALALARTAALKGALLVNYCPVRGLLHEGGKLAGLVCEDAES
                     GQRFELQARAVVNATGVWVDELRQQDGQAMGKPVRPMVAPSQGVHIVVDRDFLPSDHA
                     LLVPKTADGRVLFAVPWLGKLILGTTDSPRKDLAREPLAFKEEVDFILGESAKYLRRA
                     PGAQDVRSIWVGLRPLVKPQDEEGGNTKAISREHTVLASKSGLVTVTGGKWTTYRAMA
                     EDVLQKCFDAGLLPARAAGVTNLLRMVGTPAQPPHQRICDAQGLHSYGSEAAAVRQLP
                     GAEQWLAEGLSEAMVRFAARFEYARTVEDMLARRSRMLFLDARLAAELAPRVAAILQE
                     ELGRDPRLAEFLALAQQYQQLPT"
     gene            4652..5020
                     /gene="rplN"
                     /locus_tag="PK518_03020"
     CDS             4652..5020
                     /gene="rplN"
                     /locus_tag="PK518_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRM47911.1"
                     /translation="MIQTESRLDVADNTGAKSVLCIKVLGGSKRRYASVGDIIKVSVK
                     EAAPRGRVKKGEIYSAVVVRTAKGIRRGDGSLVKFDGNAAVLLNAKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5031..5351
                     /gene="rplX"
                     /locus_tag="PK518_03025"
     CDS             5031..5351
                     /gene="rplX"
                     /locus_tag="PK518_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRM47912.1"
                     /translation="MNKIRKGDEVIVLAGRDKGKRGTVTLRKDDSHLVIEGINLVKKH
                     VKPNPMKGTNGGIVEKAMPIHQSNVAIFNAATGKADRVGIKVQADGTRVRVFKSSGAE
                     IKAA"
     gene            5362..5901
                     /gene="rplE"
                     /locus_tag="PK518_03030"
     CDS             5362..5901
                     /gene="rplE"
                     /locus_tag="PK518_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRM47913.1"
                     /translation="MARLQKIYREKIAAELKEKFGYKSIMEVPRLTKITLNMGVSEAV
                     ADKKIMDNAVADLTKIAGQKPVVTKAKKAIAGFKIREGQAIGCMVTLRGAQMYEFLDR
                     FVTVALPRVRDFRGISGRAFDGRGNYNIGVKEQIIFPEIEYDKVDALRGLNISITTTA
                     KTDAEAKALLTGFRFPFKN"
     gene            5911..6216
                     /gene="rpsN"
                     /locus_tag="PK518_03035"
     CDS             5911..6216
                     /gene="rpsN"
                     /locus_tag="PK518_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRM47914.1"
                     /translation="MAKVALIQRELKREKLAAKYAAKYAELKAIAGDAKRSDEERDAA
                     RLGLQKLPRNANPTRQRNRCEITGRPRGTFRQFGLGRAKIRELAFAGDIPGVTKASW"
     gene            6238..6633
                     /gene="rpsH"
                     /locus_tag="PK518_03040"
     CDS             6238..6633
                     /gene="rpsH"
                     /locus_tag="PK518_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRM47915.1"
                     /translation="MSMSDPIADLLTRIRNAQMVSKATVSVPSSKVKIAIAQVLKDEG
                     YIDGFEVKTEAGKSELQIALKYYAGRPVIERIERVSRPGLRVYKGRDSIPQVMNGLGV
                     AIVTTPKGVMTDRKARATGVGGEVLCYVA"
     gene            6661..7194
                     /gene="rplF"
                     /locus_tag="PK518_03045"
     CDS             6661..7194
                     /gene="rplF"
                     /locus_tag="PK518_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRM47916.1"
                     /translation="MSRVAKSPVTIPAGVDVSIKEDQISVKGAGGSLSLAQNALVKVS
                     SNEGKLSFEPVNDSREANAMSGTVRQLVNNMVLGVSKGFEKKLTLIGVGFKAAASGSK
                     LNLNVGFSHPVNFDMPAGITVATPTPTEIVIKGANRQVVGQLAAEIRAVRPPEPYKGK
                     GIRYADEKVVIKETKKK"
     gene            7206..7571
                     /gene="rplR"
                     /locus_tag="PK518_03050"
     CDS             7206..7571
                     /gene="rplR"
                     /locus_tag="PK518_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRM47917.1"
                     /translation="MLTKKEQRLRRARQTRIRIAQQGVARLSVNRTNLHIYASVISGC
                     GTKVLASASTAEAEVRAQLGAAGKGGNVAAATLIGKRIAEKAKAAGVEKVAFDRAGFA
                     YHGRVKALADAAREAGLQF"
     gene            7587..8108
                     /gene="rpsE"
                     /locus_tag="PK518_03055"
     CDS             7587..8108
                     /gene="rpsE"
                     /locus_tag="PK518_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRM47918.1"
                     /translation="MAKFTPKVQDEGRDDGLREKMIAVNRVTKVVKGGRILGFAALTV
                     VGDGDGRVGMGKGKSKEVPAAVQKAMEEARRNMVKVSLKNGTIFHNVMGHHGAAVVQM
                     APAPKGTGIIAGGPMRAVFEVMGITDIVAKSHGSSNPYNMVRATFDALTNSTTPAEVA
                     AKRGKTVEDLFAA"
     gene            8123..8305
                     /gene="rpmD"
                     /locus_tag="PK518_03060"
     CDS             8123..8305
                     /gene="rpmD"
                     /locus_tag="PK518_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRM47919.1"
                     /translation="MTTQQTIKIQLVRSPIGTKESHRATVRGLGLRKLNSVSELKDTP
                     EVRGMINKISYLVKIL"
     gene            8316..8747
                     /gene="rplO"
                     /locus_tag="PK518_03065"
     CDS             8316..8747
                     /gene="rplO"
                     /locus_tag="PK518_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRM47920.1"
                     /translation="MELNSIKPAAGAKHAKRRVGRGIGSGLGKTAGRGHKGQKSRSGG
                     YHKVGFEGGQMPLQRRLPKRGFKSHLLKFNAEISLTALNNLGLAEVDVLALKQAGLVS
                     ELAKVVKVIKSGEISTAVKLSGVVATAGAKAAIEAAGGSLA"
     gene            8771..10090
                     /gene="secY"
                     /locus_tag="PK518_03070"
     CDS             8771..10090
                     /gene="secY"
                     /locus_tag="PK518_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRM47921.1"
                     /translation="MATNAAQLAKTGKFGDLRRRLVFLLLALVVYRVGAHIPVPGIDP
                     AQLQQLFSGQQGGILNLFNMFSGGALSRFTVFALGIMPYISASIIMQLMTYVMPTFEQ
                     LKKEGEAGRRKITQYTRYGTLGLAIFQSLGIAVALESTAGLVIAPGFGFRMTAVVSLT
                     AGTMFLMWLGEQITERGLGNGISILIFAGIAAGLPSSIGGLLELVRTGAMSILAAIFI
                     VAIVGLVTYFVVFVERGQRKILVNYARRQVGNKVYGGQSSHLPLKLNMAGVIPPIFAS
                     SIILLPATVVNWFSAGESMRWLRDIASTLTPGQPIYVLLYAAAIVFFCFFYTALVFNS
                     RETADNLKKSGAFIPGIRPGEHTARYIDKILVRLTLAGAVYITFVCLLPEFLILKYNV
                     PFYFGGTSLLIIVVVTMDFMAQVQNYMMSQQYESLLKKANFKTTPGT"
     gene            10247..10360
                     /gene="rpmJ"
                     /locus_tag="PK518_03075"
     CDS             10247..10360
                     /gene="rpmJ"
                     /locus_tag="PK518_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRM47922.1"
                     /translation="MRVSASVKKICRNCKIIRRKGVVRVICTDQRHKQRQG"
     gene            10384..10749
                     /gene="rpsM"
                     /locus_tag="PK518_03080"
     CDS             10384..10749
                     /gene="rpsM"
                     /locus_tag="PK518_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRM47923.1"
                     /translation="MARIAGINIPPHQHTEIGLTAIYGIGRTRARKICEACGIAYSKK
                     VKELTDGDLEKIRDQIAQFTIEGDLRRETTMNIKRLMDIGCYRGFRHRRGLPMRGQRT
                     RTNARTRKGPRKGAAALKK"
     gene            10765..11169
                     /gene="rpsK"
                     /locus_tag="PK518_03085"
     CDS             10765..11169
                     /gene="rpsK"
                     /locus_tag="PK518_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRM47924.1"
                     /translation="MAKSPANNAAQRVRKKVRKNVSDGIAHVHASFNNTIITITDRQG
                     NALSWASSGGQGFKGSRKSTPFAAQVASEVAGRAAIEQGIKNLDVEIKGPGPGRESSV
                     RALGALGIRITSISDVTPVPHNGCRPQKRRRI"
     gene            11262..11885
                     /gene="rpsD"
                     /locus_tag="PK518_03090"
     CDS             11262..11885
                     /gene="rpsD"
                     /locus_tag="PK518_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRM47925.1"
                     /translation="MARYLGPKAKLSRREGTDLFLKSARRSIADKAKFDSKPGQHGRT
                     SGQRTSDYGLQLREKQKVKRMYGVLEKQFRRYFEAAERRKGNTGANLLALLESRLDNV
                     VYRMGFGSTRAEARQLVSHKAITVNGQSVNIASYLVKAGDVVAVREKSKKQARIVEAL
                     QLAQQVGMPAWVEVSADKVEGTFKQIPDRDQFGADINESLIVELYSR"
     gene            11998..12993
                     /gene="rpoA"
                     /locus_tag="PK518_03095"
     CDS             11998..12993
                     /gene="rpoA"
                     /locus_tag="PK518_03095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRM47926.1"
                     /translation="MQTNLLKPKAINVEQLGHNRAKVELEPFERGYGHTLGNAIRRVL
                     LSSMVGYAATEVTIAGVVHEYSSIDGVQEDVVNILLNLKGVVFKLHNRDEVTLSLRKD
                     GEGVVTARDIQTPHDVEIINPDHVIATLSAGGKLDMQIKVEKGRGYVPGNLRRYADEA
                     TKSIGRIVLDASFSPVKRVSYTVESARVEQRTDLDKLVLEIETNGAISAEEAVRASAK
                     ILVEQLAVFAQLDGGEIFEGQPRGSHPPSGATFDPILLRPVDELELTVRSANCLKAEN
                     IYYIGDLIQRTENELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLDKR"
     gene            13084..13473
                     /gene="rplQ"
                     /locus_tag="PK518_03100"
     CDS             13084..13473
                     /gene="rplQ"
                     /locus_tag="PK518_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRM47927.1"
                     /translation="MRHGHGLRKLNRTSSHRLAMLQNMMNSLIEHEAIKTTVPKAKEL
                     RRVIEPMITLAKEDSVANRRLAFNRLRDRDSVTKLFNDLGPRFKTRPGGYTRILKMGF
                     RVGDNAPMAFVELVDRPEVADETNNEA"
     gene            13498..13574
                     /locus_tag="PK518_03105"
     tRNA            13498..13574
                     /locus_tag="PK518_03105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13532..13534,aa:Met,seq:cat)
     gene            13666..14418
                     /locus_tag="PK518_03110"
     CDS             13666..14418
                     /locus_tag="PK518_03110"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRM47928.1"
                     /translation="MADFSRSLDLSGASNFRDLGGYASQDGRQIRWRRIFRSDHLASL
                     TPQDRQLLAGLGVARAVDFRGQAESAALSYELPHVRYQALPIEPIVVQRAKEMALAGH
                     SMTAPIAVRLMQDTYRAFVSDNTSQFASLFEQLLLEDTPLVFHCTAGKDRTGFAAALV
                     LLALGVSREVVMQDYLLTNGLYRRPAGLVSSAPEEVLNVIWRVQAEFLQAALQAVDHD
                     HGGVQRYLKHRLGMHEAATKRLAELYLQPHAA"
     gene            complement(14475..14906)
                     /locus_tag="PK518_03115"
     CDS             complement(14475..14906)
                     /locus_tag="PK518_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRM47929.1"
                     /translation="MATAKKTTAPVKKTTASAKKAAEPAKTAAAKKTAAPAAPKPVKD
                     PFNKTTLVAHLASQAGVEPKTAKAVLAALETAILGAVHKKGCGEFTLPGLLKIGLLQV
                     PAKKKRFGKDPFTGEERWFPAKPASVKIKTRALKKLKDATL"
     gene            15148..15921
                     /locus_tag="PK518_03120"
                     /pseudo
     CDS             15148..15921
                     /locus_tag="PK518_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15975..17210
                     /locus_tag="PK518_03125"
     CDS             15975..17210
                     /locus_tag="PK518_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HRM47930.1"
                     /translation="MKLFPNAHATHPQWRMAAALVLAQLQAQMAQPQYARKPSLGLLY
                     ITDHYVADAVALLRYLGQQLPSVADWSGTVGVGVAGNNVEYFDEPALSIMLCDLPPEQ
                     YRVFSGVAPLPLGASAFSAHTALVHADGRTPDLAELLVEMAGRTSSGYLFGGVAASRG
                     ASLAFAQHAGGDGAASGVLSGGLSGVAFSDQVGLVSRVTQDCQPIGPQAVVTQAQDNV
                     VLRLDGQPALDVLLQTLGVELNGDPQPALRAVRHTLAGLSNADAPALQGTGHFGADTR
                     VRHIVGLDVARGGVALADRVHEGMHLAFCQRHMAAARADLTRICAEIREELAPESPAL
                     PDEQAATQRRICGALYVSCSGRGGRHFGGPSAELQTVHRALGDVPLVGFFASGEIAAH
                     WLYGYTGLLTVFVAPADTA"
     gene            complement(17251..18357)
                     /locus_tag="PK518_03130"
     CDS             complement(17251..18357)
                     /locus_tag="PK518_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806972.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRM47931.1"
                     /translation="MTDTIARLTHLQDTPQTQATRQQRAKAISATQACEDLLLAPDLP
                     GGLPLAQRLAVAQTVSAASGVTALGEHYAAQLQRLPTATPDARWQAMHHFTQLLATQP
                     AAADRAALLALPAAGLTTADVVLLAQLIGFAAFQARLVAGVAALAALGPAGSTATPAA
                     PAAEALFIHPANLPAPGEPLRLNGYTSETLDWKAWLPVVDATQASAEQNAVLDASHPK
                     ARVSDFYLLLVHQPRVLAERSEAFNAIMYAPGGLPRSERELATAVVSRINGCVYCASV
                     HAQRFEQLAKRNDVIAQVFIDPDQAGTNARERAIVQASAALTRAPGSFGAHHLAALRD
                     AGLSDLEILDTVHAVALFAWANRLMLNLGEAVYP"
     gene            complement(18354..19841)
                     /locus_tag="PK518_03135"
     CDS             complement(18354..19841)
                     /locus_tag="PK518_03135"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM47932.1"
                     /translation="MPDTPALGAAGLAALEERLAHDLLTLNWRARPWLPALSHGGQPV
                     IDVLIIGAGQAGLAASMALAQQGIAAVLLDRAATDYEGPWATTARMETLRSPKELTGP
                     ALGLPSLTFRAWFEAQWGRSAWDALDKIPRLQWMDYLRWYRRVTQADVRNEHAVTAVR
                     PRADALVEVDVHTPTCSATWLARRVVLATGRDGLGGAQIPAFMDGVGHASKGGLWAHS
                     SDVLDYATLAGKRVGVIGMGSSAMDSAGTALECGAASVDLIARRTAMPTINKSKGSGL
                     PGLTQGHHELPDAWKWRIRHYINVQQVPPPRGSTLRVSRHAHAFFHFGCAVQQVTQQG
                     QALQVATSQGRFAFDFLILATGFAIDWAQKPEFAAFAPHVRSWGERYQSPPGEDDREL
                     FDSPDLGPVFELREKSPGACPGLDRIHCFCYPAALSHGTVSGDIPAISDGARRLASGI
                     AGLLYAEDVEQHFAALQAYAEPELLGDEWTPADTRQRVLPESPPT"
     gene            complement(19862..20854)
                     /locus_tag="PK518_03140"
     CDS             complement(19862..20854)
                     /locus_tag="PK518_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM47933.1"
                     /translation="MHPILRRTALAATALLFASSAALAQANGWPKQPITLIMGFPAGS
                     GVDVVARAVQEPLGQKLGQPIVIDYKSGAAGNIASEYVAHAKPDGYTLAFGTAATHGS
                     NAALYKSLPFDVEADFVPVGPLIDVSNVLTINPSVIDANNLKEFVEIVKANPGKYNYA
                     STGNGAGTHMAFAEFNARLGLDMVHVPYKGGPEAITSVLRGETCCIMNQVQTVLAHYK
                     SGKVRLLGITTAKPVDAVKEVPTIAASGLPGTKGFDSSIWFGIFAPKGTDPTVVNKLN
                     TALRQVLELPEIRQRFESQGNTVRIETPEQFRQTVRKDRAKWAQVVKDAKISIN"
     gene            complement(20987..21397)
                     /locus_tag="PK518_03145"
     CDS             complement(20987..21397)
                     /locus_tag="PK518_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HRM47934.1"
                     /translation="MAYPLTHALARPLGLAAATLTALLALAGAAHAAPGVPQHQTHAA
                     QPAHYRPAPPPPRYERAPAARRGMAWSQGYWQWRGQRYAWVPGHWVQARHGQHYRQPG
                     WEQRQGQWHFTGGGWGHGGRPAPQGPRPGGSHRH"
     gene            21680..23584
                     /locus_tag="PK518_03150"
     CDS             21680..23584
                     /locus_tag="PK518_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM47935.1"
                     /translation="MSAPHPNQFALLGQRRFAPFFWTQAAGAANDNLFKFAFTVMVTY
                     QLGVSWLPPSLAGLVIGALFILPFLLFSATAGQITDKLEKTRIIRFVKNLEIAIMLLA
                     ALGFMAGHAALLLACVFLMGLHSTLFGPVKFAYLPQVLSERELTGGNGMVEMGTFVAI
                     LLGNVAGGLLVAMPGVGHGAVALACLGLALAGRLAAQFIPGAPATDPGLVVNWNPVSE
                     TWRNLQLAHGNIVVFRSLLGISWMWFFGAVFLSQFPSFAKDVLRGDEQVASALLVVFS
                     LGIGTGALLCEVLSRRHIEIGLVPLGAIGMSVFAGDLYFASRGLPPSGVMALGAFLTQ
                     PAYWRVMLDLFLLSLFTGLYSVPMYALIQMRSQATHRARIIAANNILNALFMIVSAVL
                     AGLLLQAGFTVPQIFLFTAIANAVVAAYIFLLVPEYLLRFVAWVLSRCVYRFRVRGDE
                     HIPTAGAAILACNHVSFVDAVLLMAASPRPIYFLMDHRIFRVPVLGRLFRLAKAIPVA
                     PQKDDPATYQAAFERAAQVLKEGDLLAIFPEGGITRDGQLQEFKGGIMKIIERAQADG
                     VQAPVVPMALTNLWGSFFSRIEQGGAMVRPFRRGMFTRVGLNVGAPLAAAEVRPEPLR
                     ARVAGLLALG"
     gene            complement(23591..24007)
                     /locus_tag="PK518_03155"
     CDS             complement(23591..24007)
                     /locus_tag="PK518_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRM47936.1"
                     /translation="MSTDRLPPPPWERIVADVADALIFIDRAGVIRAWNAGAEALFGF
                     AAVQTLGQSVDMIIPPHLREAHWRGFERAMQRGACSRPAEVRTTRGLHQDGRRLYVDM
                     SFSVVKDAAGQVLGSAAMARDATARYLAEQAMRAGG"
     gene            24139..25200
                     /locus_tag="PK518_03160"
     CDS             24139..25200
                     /locus_tag="PK518_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM47937.1"
                     /translation="MGKALRLSEKWFRRGLWLVALVFAGFLIGLGGTIVGDLPQVEQP
                     LLLDDFLDSGAATALRGQIQQAQQAEQDAQAALEQARLRHARARSATQAERETLANWL
                     AARSATQRAEHDPEVIARTQALDALKATELQAQQAVQAQDQALLDARQGGQAAQQRLN
                     AMERDAHDKLAAERRRVELRVFLYRLALTLPLLAVAGWLFAKKRQGTWWPFVWGFIFF
                     ALFAFFVELVPYLPSYGGYVRYIVGIALTALVGRQAIVALNRYLERQRRAEAMPAPQR
                     RQGLGYDLALARLAKNVCPGCERPVDLKDGKTDFCPHCGIGLFDHCGHCNARKSAFAR
                     FCQACGQAAGGVAGGYEAP"
     gene            complement(25339..26766)
                     /locus_tag="PK518_03165"
     CDS             complement(25339..26766)
                     /locus_tag="PK518_03165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM47938.1"
                     /translation="MSTPPIATQQSAAPWRDPLLLLASVFAACLLGIASRPAGYSAAI
                     WPANAVLLGLLLRNPSWARGPTAWICALVGYLTADMLTGAPWLRALGMNGANLLGVAV
                     GWLFFRPYAASIVGFTRQRAVLTLLAGSSVAALGSALPATPVMQWAFASQAWTDMLMW
                     LSSEMYNLLLFLPLVLAAPRGWFWQWDWARLLRPLRQAGVAPLLALLACEALAYFVNG
                     PGMLGFAVPALVWCAMAYGVFPITVLNLLVCSWKTAVAALDTISFLPDQLAAVTSFRL
                     GMALLSLAPLAVAVAHQLRSQTLSQLQQAVDHDHLTGALSRRKLMEQGARLVQRLHQQ
                     GAPVAVLLMDLDHFKRINDSHGHAQGDTVLREFAALVQRNLRPQDLFGRIGGEEFALI
                     LPATAASHAQLIGERLRSQLREHPFRLQDGSALQVTLSVGLHATQPPDQQHSLERLLA
                     WADAALYQAKALGRDQVRGHWADQG"
     gene            complement(26864..28036)
                     /gene="tgt"
                     /locus_tag="PK518_03170"
     CDS             complement(26864..28036)
                     /gene="tgt"
                     /locus_tag="PK518_03170"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRM47939.1"
                     /translation="MLQFDLLKTDPSSHARRGRLTLNHGVVQTPIFMPVGTYGTVKGV
                     MPRSLHEMGAQIILGNTFHLWMRPGLDIVQSFGGLHRFEQWDKPILTDSGGFQVWSLG
                     AMRKITEEGVTFASPVNGDKLFMSPEVSMQIQTILNSDIVMQLDECTPYETNGHKTTE
                     AEARKSMEMSRRWALRSKTEFERLANPNALFGIVQGGMFPHLRQESLDALVEMDFPGY
                     AIGGVSVGEPKEEMLAIMAHTPHRLPQHKPRYLMGVGTPEDLVEGVACGVDMFDCVMP
                     TRNARNGTLFTRHGDLKIRNARHKSDHQPLDASCRCHACAGSEGVAWQDGGRGGFSRA
                     YLHHLDRCGEMLGPMLTTIHNLHYYLNLMQEIRDALDAGRFAEFRARFKADRARGV"
BASE COUNT         4905 a         8891 c         9375 g         5016 t
ORIGIN      
        1 tcctgggcga caagcatgtg ttcttctggc tgctgaccaa ccgcatgacg ccgccggcgg
       61 tgttcctgct gcccttcttc cagctctaca ccaccgtggg cctgatggac acgcacatcg
      121 ccgtcgccct ggcgcacctg ctgttcaacg tgccgctggc ggtgtggatc ctggagggct
      181 tcatgagcgg catcccgcgc gagatcgacg agacggcgta catcgacggc tacagcttcc
      241 cgcgcttctt cctcaccatc ttcctgccgc tcatcaaggc cggcgtgggc gtggcggcat
      301 tcttctgctt catgttctcg tgggtggaat tgctcttggc gcgcacgctc accagcgtga
      361 acgccaagcc catcgtcgcc accatgacgc gcaccgtgag cgccagcggc atggactggg
      421 cgacgctggc cgccgccggg gtgctgacca tcgtgcccgg ggcgatcgtc atctggtttg
      481 tgcggcatta catcgccaag ggctttgcca tggggagggt gtgacatgtt cgagtggatg
      541 gcctggacca cgccggtggc cgttttcttc acctgcatcg tgctcatgct catcggcatg
      601 acggtgtggg agatccgctc tcccacggtg gagcgcaagg gctttctgcc catggccacc
      661 acgcgcggcg accggctgtt catcggcctg ctcaccgccg cgtacatcaa cctggcctgg
      721 gtgggcctgg gcgagaagat ggcccaatgg ttctcactgg aggccgagcc ctcggtgtgg
      781 atcagcttcg tgctctccat gctggtgctg gtgctggtgc tgcgcaaggg ttgatatgaa
      841 aacgatagct tccggcgctt gttgaaatag caattcagag tgttttgctc tgtaaatggc
      901 tttcagcaag cgccagcagc tctgattttg atagtagcaa agggccgacc cgttcctccc
      961 ccgaggtcac aggcccatcc aacaatgggg agtctgacgt gaggagacag caatgaagat
     1021 gcagttcacg gcgatcgcgt tcgccaccgc agccatggcc ctgggccagg cggcctgggc
     1081 cggcgaggcc gaagccaaga agtggatcga cagcgagttc cagccctcga cgctgagcaa
     1141 ggatcagcag atggccgaaa tgaaatggtt catcgaggcg gccaagaagc tccaggccaa
     1201 gggggtgaag gagatctccg tcgtctccga gaccatcacc acgcacgaat acgagtccaa
     1261 gaccctggcc aaggcctttt ccgagatcac cggcatccag gtcaagcacg acctgatcca
     1321 ggaaggcgac gtggtcgaga agctgcagac ctccatgcag tcgggcaagt ccatctacga
     1381 cggctggatc agcgactccg acctcatcgg cacgcactac cgctacggca agatcctgaa
     1441 cctgaccgac tacatggccg gcgccggcaa ggactacacc aaccccgggc tggacctcaa
     1501 ggacttcatc ggcaccaagt tcaccaccgc gcccgacggc aagctctacc aattgcccga
     1561 ccagcagttc gccaacctgt actggttccg cgccgacctg ttcgaccgca aggacctcaa
     1621 ggacaagttc aaggccaagt acggttacga cctgggcgtg ccgctgaact ggtctgccta
     1681 tgaggacatc gccgagttct tcaccaacga cgtcaagcag atcgacggca agcccatcta
     1741 cggccacatg gactacggca agaaggaccc cagcctgggc tggcgcttca ccgacgcctg
     1801 gctgtccatg gccggcagcg ccgacatcgg cacgcccaac ggcctgccca tcgacgagtg
     1861 gggcatccgc gtggccgacg acaagtgcac ccccgtgggc gccagcgtgg cccgtggcgg
     1921 cgccaccaac tcgccggccg ccgtctacgc gctcaccaag tacgtggact ggatgaagaa
     1981 gtacgcgccc aaggaagcca tgggcatgac cttcggcgaa gccggccccg tgcccgccca
     2041 gggccagatc gcgcagcaga tcttctggta cacggccttc accgccgaca tgaacaagcc
     2101 cggcctgccc gtggtcaacg ccgacggcac gcccaagtgg cgcatggccc ccggccccaa
     2161 cggcccctac tggaagcagg gcatgcagaa cggctaccag gacgtgggca gctggacctt
     2221 cttcaaggac cacaaccccg accgcctggc cgccgcctgg ctctacgcgc agttcgtgac
     2281 cgccaagacc acctcgctga agaagaccgt ggtcggcctc acgcccatcc gcgagtccga
     2341 catccgctcc aaggccatga ccgacatggc gcccaagctt ggcggtctgg tcgagttcta
     2401 ccgcagcccg gcgcgcgtgg cctggtcgcc caccggcacc aacgtgcccg actaccccaa
     2461 gctcgcccag ctctggtgga agaacgtggc ccaggccgtc acgggggaga agaccccgca
     2521 ggccgccatg gacacgctgg ccgacgagat ggaccaggtc atggcccgcc tggagcgcgc
     2581 cggcatggag cgctgcgcgc ccaagctcaa caagaaggaa gaccccaaga agtggctgag
     2641 cgacaagcac gcaccctggg ccaagctggc caatgaaaag cccaagggcg agaccatcaa
     2701 ctacaacacc ctgctgcagg cctggaagga cggcaaggtg cgttgatgcc gcagcggatg
     2761 ggccctttgc cgggcccatc ccgcggatcg ggtcacaatt cagggtaaac cctgattttt
     2821 ccgccagggt tacggaccat tccccttatg actagcgaca cctccacccc tttggcaacc
     2881 gtacgggccg acctgctggc ccgcctggct caacccgtga cctacgacct ggcggtcgtc
     2941 ggaggtgggg cgacgggcct gggggtggcg ctggatgcgg cggcgcgcgg cttttccgtc
     3001 gtgctgctgg aatcgcacga cttcgccaag ggcacctcct cgcgtgccac caagctggtg
     3061 cacggcggcg tgcgctacct ggcgcagggc aatatcgccc tggtgcgcga ggcgctgcac
     3121 gagcgcacca cgcttttgca caacgcgccg cacctggcgc agccgctggc cttcgtcatg
     3181 ccctcgtaca agctgtggga gacgccgttc tacggcgtcg gcctgaagat gtatgacgcg
     3241 ctggcgggcc aggctggcct gggctcgacg caattcctcg ggcgcatgga aacactgcgc
     3301 tgcctgccca cggcgcgcgc tgacgggctc aagggcggcg tcaagtactg ggacggccag
     3361 tttgacgatg cccgcctggc gctggcgctg gcgcgcacgg cggcgctcaa gggtgcgttg
     3421 ctggtcaact attgcccggt gcgcggcctg ctgcacgagg gtggcaagct ggctggcctg
     3481 gtctgcgagg atgctgaaag cggccagcgc tttgagctgc aggcccgcgc cgtggtcaac
     3541 gccaccggcg tctgggtgga cgagttgcgc cagcaggacg gccaggccat gggcaagccg
     3601 gtgcgcccca tggtcgcgcc cagccagggc gtgcacatcg tcgtcgatcg cgattttctg
     3661 ccctcagacc acgcgttgct ggtgcccaag acggcagacg gtcgcgtgct gtttgccgtg
     3721 ccctggctcg gcaaactcat cctggggacg acggacagcc cgcgcaagga tctggcgcgt
     3781 gagccgttgg ccttcaagga agaggtggat ttcatcctcg gcgaatcggc caagtacctg
     3841 cgccgtgcac ccggggcgca ggacgtgcgc agcatctggg ttggcctgcg cccgctcgtc
     3901 aagccgcagg atgaagaggg tggcaacacc aaggccatca gccgcgagca cacggtgctg
     3961 gcgagcaaga gcggcctggt caccgtgacg ggcggcaaat ggaccaccta ccgggccatg
     4021 gccgaggatg tgttgcaaaa atgcttcgac gcgggactgc tgccggcgcg tgcggccggc
     4081 gtgaccaacc tgttgcgcat ggtgggcacg ccggcgcagc cgccacacca gcgcatctgc
     4141 gacgcccagg gtttgcattc ctatggcagc gaggctgcgg ccgtcaggca gctgcccggt
     4201 gcggaacaat ggctggccga ggggctgagt gaagccatgg tgcgctttgc tgcgcgtttt
     4261 gagtacgccc gcaccgtgga ggatatgttg gcgcggcgca gccgcatgct gttcctggat
     4321 gcgcgcctgg ccgccgagct ggcaccacgc gtggcggcca tcctgcagga agagctgggc
     4381 cgtgatccac ggctggcgga gttcctggcc ctggcgcagc agtaccagca actgccgacc
     4441 tgagcatttg gccgccagtt gttgactttg atttccagct gggtcacaat ccgaggcttc
     4501 gcgtgaaaag cgcgaggttt ctgcccagtg gaagcgctgc gaaatggttc gcggcgtgga
     4561 cgacgggccc aagactgact ggctgcctgg cggcccttga gaaggactct ggggctgtcg
     4621 ggatgtgctg gtgcaagttg ggaatattga aatgatccaa acagaatctc ggttagacgt
     4681 tgccgacaat accggcgcga agtccgtcct gtgcatcaag gtgctgggtg gttccaagcg
     4741 tcgctatgca agcgttggcg acatcatcaa ggtgagcgtg aaggaagctg ctccgcgtgg
     4801 tcgcgtcaaa aaaggcgaga tctacagtgc tgtggtcgtt cgtacggcca agggcattcg
     4861 ccgcggcgac ggttcgctcg tcaaattcga tggcaacgcg gcagtgttgc tgaatgcaaa
     4921 gctggagccc atcggcaccc gcatcttcgg ccccgtgacg cgtgagctgc gtaccgagcg
     4981 tttcatgaag atcgtgtccc tggctcccga agttctctga aggacgcacc atgaacaaga
     5041 tccgcaaggg cgacgaggtc atcgtgctcg ccggccgcga caagggcaag cgtggcacgg
     5101 tcacgctgcg caaggatgac tcccatctgg tcatcgaagg catcaacctg gtcaaaaagc
     5161 atgtcaagcc gaaccccatg aagggtacca acggcggcat cgtggaaaag gccatgccga
     5221 ttcatcagtc caacgtggcc atcttcaatg ccgctaccgg caaggctgat cgcgtgggca
     5281 tcaaggtgca ggccgacggt acgcgtgtgc gcgtgttcaa gtccagcggc gccgaaatca
     5341 aggccgccta aggagtagca catggcacga ctgcaaaaaa tctatcgcga aaagatcgcg
     5401 gccgaactca aggaaaagtt tggctacaag tcgatcatgg aagttccgcg cctgaccaag
     5461 atcaccctga acatgggcgt gagcgaggct gtggccgaca agaagatcat ggacaacgcc
     5521 gttgccgatc tgaccaagat cgccggccag aagcccgtgg tgaccaaggc caagaaggcc
     5581 atcgccggtt tcaagatccg cgaaggccag gccatcggct gcatggtgac cctgcgtggc
     5641 gcgcagatgt atgagttcct ggatcgtttc gtgaccgtgg ccctgccgcg tgtgcgtgac
     5701 ttccgtggta tctccggccg cgccttcgat ggccgtggca actacaacat cggcgtcaaa
     5761 gagcagatca ttttccctga gatcgagtac gacaaggtgg atgccttgcg tggcctcaac
     5821 atcagcatca ccacgacggc caagaccgac gcagaagcca aggcgcttct gaccggcttc
     5881 cgtttcccgt tcaagaacta aaggcagcgt atggctaaag tagctttgat ccagcgcgaa
     5941 ctcaagcgcg aaaaactggc cgccaagtac gccgccaagt acgcggaact gaaggcgatt
     6001 gccggcgatg ccaagcgcag cgacgaagag cgcgacgctg cacgcctggg gctgcagaag
     6061 ttgccccgca acgccaaccc cacgcgtcaa cgcaaccgct gcgaaatcac cggtcgtccc
     6121 cgtggcacct tccgtcaatt cggtctgggc cgcgccaaga tccgtgaact ggcattcgcc
     6181 ggtgacatcc ccggtgtcac caaggccagc tggtaagcga agcaggagag atacaacatg
     6241 agcatgagtg atcccatcgc tgacttgctg acccgcatcc gcaatgcgca aatggtgtcc
     6301 aaggccaccg tttcggtgcc ttcttccaag gtgaagattg ccatcgcaca ggtgttgaag
     6361 gacgagggtt acatcgacgg cttcgaagtc aagaccgaag ccggcaagtc cgagctgcaa
     6421 atcgccctga agtactacgc cggtcgcccc gtgatcgagc gcatcgagcg cgtcagccgt
     6481 cccggcctgc gcgtgtacaa gggtcgcgat tccattccgc aagtcatgaa cggcctgggt
     6541 gtggcgattg tcaccacgcc caagggtgtg atgaccgatc gcaaggcgcg cgctaccggt
     6601 gtcggcggcg aagtgctttg ctacgtcgct taagcgcggg catgaaggag aaatactgaa
     6661 atgtctcgcg tagcaaaatc cccggtgacc atccccgcag gcgtggatgt gtccatcaag
     6721 gaagatcaga tcagtgtcaa gggtgcaggc ggctcgctgt cgctggcaca gaatgcactg
     6781 gtgaaggtct ctagcaatga aggcaagctg agcttcgagc ccgtcaatga ctcgcgtgaa
     6841 gccaatgcca tgagcggtac cgtgcgccag ttggtgaaca acatggtgct gggcgtcagc
     6901 aagggcttcg aaaagaagct gacgctgatc ggcgtgggct tcaaggcggc agcctccggc
     6961 tccaagctga atctgaacgt gggtttctcg caccccgtca actttgacat gcccgccggc
     7021 atcaccgtgg ccacccccac gccgacagaa atcgtgatca agggtgcgaa tcgccaggtg
     7081 gtgggccagc tggccgccga gatccgcgcc gttcgtcctc ccgagcccta caagggcaag
     7141 ggcatccgtt atgcggatga gaaggtcgtg atcaaagaga ccaagaagaa gtaaggagct
     7201 gcaatatgtt gaccaagaaa gagcagcgtc ttcgtcgtgc ccgtcagaca cgtatccgca
     7261 ttgcacagca aggcgtcgcg cgtctgtcgg tgaaccgtac caacctccac atctacgcca
     7321 gcgtgatttc tggctgcggc accaaggtgc tggcatcggc ctcgacggcc gaagcagaag
     7381 tgcgtgccca gctcggcgcc gccggcaagg gtggcaacgt ggccgccgcc acgctgatcg
     7441 gcaagcgcat tgccgagaag gccaaggctg ccggtgttga gaaggttgcg tttgaccgcg
     7501 cgggttttgc ctaccacggt cgcgtcaagg ccctggccga cgcagcccgt gaagcaggtc
     7561 tgcagttcta agcggagcgg ataaaaatgg ctaaatttac ccccaaggtg caagacgaag
     7621 gtcgtgacga cggtctgcgc gaaaaaatga tcgcggtcaa ccgcgtgacc aaggtcgtga
     7681 agggcggtcg tatcctcggc ttcgctgcac tgaccgtggt tggcgacggt gacggccgcg
     7741 ttggcatggg caagggcaag tccaaggaag tgcccgctgc cgtgcagaag gccatggaag
     7801 aggcccgccg caacatggtg aaggtgtcgc tgaagaacgg caccatcttc cataacgtga
     7861 tgggtcacca cggcgccgcc gtggtgcaga tggcgccggc tcccaagggt accggcatca
     7921 tcgccggcgg cccgatgcgc gccgtgttcg aggtgatggg cattaccgat atcgtggcca
     7981 agagccacgg ttcgtccaac ccctacaaca tggttcgtgc aacgttcgat gccttgacca
     8041 attccacaac gccggccgaa gtggccgcca agcgtggcaa gacggtcgaa gacctcttcg
     8101 ctgcctgata gggaggcgac caatgacaac gcaacaaacc atcaagattc aactggtgcg
     8161 cagcccgatt ggcaccaagg aatcgcaccg cgccaccgtg cgtggcctgg gtcttcgcaa
     8221 gctcaacagc gtcagcgaac tcaaggacac acccgaggtg cgcggcatga tcaacaagat
     8281 cagctatctg gtcaaaatcc tctgaaaggt tgatgatgga actcaatagc atcaagcctg
     8341 cagcgggtgc caagcacgcc aagcgccgtg tcggccgtgg catcggctcc ggcctgggca
     8401 agaccgccgg tcgcggtcac aagggccaga agtcgcgttc cggcggctac cacaaggtgg
     8461 gcttcgaagg cggccaaatg cctttgcagc gccgtctgcc caagcgcggt ttcaagtcgc
     8521 acctgctgaa gttcaacgcc gagatttctc tgacggccct gaacaatctc ggcctggccg
     8581 aggttgacgt gctggcactg aagcaggctg gcctggtcag cgagctggcc aaggtcgtga
     8641 aggtcatcaa gagtggcgag atctccacgg ccgtgaagct gtcgggcgtc gtcgcgaccg
     8701 ccggtgccaa ggctgccatc gaggccgccg gtggcagcct ggcctgattt ggttctgaag
     8761 aaaggcatct gtggctacta acgcagctca actggcaaaa accggaaagt tcggcgatct
     8821 gcgccggcgc ctggtgttct tgttgctcgc gttggtggtg taccgcgtgg gggcgcatat
     8881 tcccgttccg ggaatcgacc ccgcgcagct gcagcagctg ttcagtggcc agcagggtgg
     8941 catcctcaac ctgttcaaca tgttctcggg cggggcgctg tcgcgcttca cggtcttcgc
     9001 gctggggatc atgccgtaca tctcggcatc gatcatcatg cagttgatga cctatgtgat
     9061 gcccacgttc gagcagttga agaaggaggg cgaggccggc cgccgcaaga tcacgcagta
     9121 cacccgctac ggcacgttgg gcctggccat ctttcagtcg ctcggcatcg ccgtggcgct
     9181 ggagagcacg gccgggctgg tgatagcgcc gggctttggc tttcgcatga cggctgtggt
     9241 cagcctgacg gcgggcacca tgttcctgat gtggctcggc gagcagatca ccgagcgcgg
     9301 cctgggcaac ggcatttcga tcctgatttt tgcaggtatc gcagccggcc tgccgagctc
     9361 catcggtggc ctgctggagt tggtgcgcac gggtgccatg agcatcctgg cggccatctt
     9421 catcgtggcc atcgtcggct tggtgaccta cttcgtggtc ttcgtcgagc gtggacagcg
     9481 caagatcctg gtgaactacg cgcgccgtca ggtgggcaac aaggtctatg gtggacagtc
     9541 gtcgcatctg cccttgaagc tgaacatggc aggcgtgatc cctccgatct ttgcttcgtc
     9601 catcatcttg ctgccggcta ccgtggtgaa ctggttcagt gcgggtgaat ccatgcgctg
     9661 gttgcgtgac atcgcctcca cgctgacacc cggacaaccg atctatgtgc tgctgtacgc
     9721 tgccgccatc gtgtttttct gcttcttcta tacggcgttg gtcttcaaca gccgcgagac
     9781 cgcagacaac ctgaagaaga gcggggcatt cattcccggg attcgaccag gtgaacacac
     9841 ggcccgctac atcgacaaga ttctggtacg cctgacgctg gccggagccg tgtacatcac
     9901 cttcgtgtgc ctgctgcccg agttcctgat tctgaagtac aacgtgccgt tctattttgg
     9961 tggcacctcg ttgctcatca tcgtggtggt gacgatggac ttcatggctc aggtgcagaa
    10021 ctacatgatg tcgcagcagt acgaatcgct gctcaagaag gccaacttca agacgacacc
    10081 aggtacctga caccggcgtg gctttggtgg atgcgttata gtcgcgggtt cgccaaagaa
    10141 gcaacggttt ttcgatgccc ttcgatccat cgcgggcaca tgttttgtgt tccgcgcgca
    10201 ttgagtaagt ggccggaacg attggacaag agtttaggag aatgcaatga gagtttcggc
    10261 ttcggtcaag aaaatttgcc gcaactgcaa aattatccgc cgcaagggcg ttgtgcgcgt
    10321 gatctgcacg gatcagcgtc acaagcagcg ccaaggttga ttgggaaata ttagaggacg
    10381 cacatggctc gtatcgctgg tatcaacatc ccgccccatc agcacaccga gattggcctg
    10441 acggccatct acgggattgg gcgtacccgt gctcgcaaga tttgcgaggc atgcggaatt
    10501 gcttattcga agaaggtcaa ggaattgact gacggcgacc tggaaaagat tcgtgaccag
    10561 atcgcccagt tcaccatcga aggtgacctg cgtcgtgaaa ccacgatgaa catcaagcgc
    10621 ctgatggaca tcggctgcta tcgcggtttc cgccatcgcc gtggcctgcc catgcgcggt
    10681 cagcgtacgc gcaccaatgc ccgtacccgc aagggcccgc gcaagggtgc ggcggcactg
    10741 aagaagtaag actgaaagat caatatggcc aagtctcctg ccaataacgc tgcgcagcgt
    10801 gtgcgcaaga aggttcgcaa gaacgtgtcg gatggcattg cccacgtgca tgcctccttc
    10861 aacaacacca tcatcaccat caccgatcgc cagggcaatg ctctgtcctg ggcttcgtcc
    10921 ggtggccagg gtttcaaggg ttcgcgcaaa tcgaccccgt ttgccgcaca ggtcgcctct
    10981 gaagtggccg gtcgtgccgc catcgagcag ggcatcaaaa accttgacgt cgagatcaag
    11041 ggtcccggcc ctggtcgcga gtcgtcggtg cgcgccctgg gtgccctggg catccgcatc
    11101 acgtccattt cggatgtgac gccggttccg cacaacggct gccgcccgca aaagcgccgc
    11161 cgcatctgat tttttcagta agcccaccgc caccgatcgc ctggtgcgat ggtggctccc
    11221 gcaggtttct gcggtagttg attagttcca aggatataca agtggcacgt tatctcggcc
    11281 cgaaggccaa actctcccgc cgcgaaggca ccgacctgtt cctgaagagc gcgcgtcgct
    11341 cgatcgccga caaggccaag ttcgactcca agcctggcca gcatggccgc acttcgggtc
    11401 agcgtacctc cgactacggt ttgcagctgc gcgagaagca aaaggtcaag cgcatgtacg
    11461 gcgtgctgga gaagcagttc cgccgctact tcgaagccgc cgagcgccgc aagggcaaca
    11521 ccggtgccaa cctgctggcg ctgctggaat cgcgcctgga caacgtggtg tatcgcatgg
    11581 gtttcggctc gacgcgcgcc gaggcacgcc agctggtgtc gcacaaggcc atcacggtga
    11641 atggtcaaag tgtgaacatt gcctcctacc tggtgaaggc cggtgacgtt gtcgccgtgc
    11701 gcgaaaagtc aaagaagcag gcccgtatcg ttgaggcact gcaactggcg cagcaggtgg
    11761 gcatgcctgc gtgggtggaa gtaagcgccg acaaggtcga aggcaccttc aagcaaatcc
    11821 ccgatcgtga tcagttcggt gcggacatca acgaatccct gatcgttgag ttgtactcgc
    11881 gttaatcctg aagccaccgt tagcgtaaat cgttcctgaa ccgcgaggca tcgcggttta
    11941 ggtgcttcat cagccttacc ggtgtaacga gctgggggca ttgagaagga agtctgcatg
    12001 caaaccaatt tgctgaaacc caaggcaatc aacgtcgagc aacttggcca caaccgggcc
    12061 aaggtcgagc tggagccgtt cgagcgtggc tatggccaca cgctgggcaa cgccattcgc
    12121 cgtgtcctgc tctcgtccat ggtgggttac gcagcaaccg aggtgaccat tgccggagtc
    12181 gtgcacgagt actcgtccat cgacggcgtg caggaagacg tggtcaacat cctcttgaat
    12241 ctcaaagggg tggtgttcaa gctgcacaac cgtgatgagg tcaccctgag cctgcgcaag
    12301 gacggcgaag gtgtcgtgac cgcacgcgac atccagaccc ctcatgacgt cgagatcatc
    12361 aatcccgatc acgtcatcgc cacactgtcg gccggcggca agctggacat gcagatcaag
    12421 gttgaaaagg gtcgcggcta cgtgcccggg aacctgcgcc gctatgccga cgaggccacc
    12481 aaatccatcg gccgcatcgt gctggatgca tcgttctcgc cggtgaagcg ggtgagctat
    12541 acggtggaaa gcgcccgcgt tgagcagcgt accgacctgg acaagctggt tctggagatc
    12601 gaaaccaacg gcgccatttc ggccgaggag gccgtacgcg cttctgccaa gatcctggtc
    12661 gagcaactgg ccgtattcgc gcagctcgat ggcggcgaga tcttcgaagg tcagccgcgg
    12721 ggttcacatc ctcccagcgg cgccacgttt gatccgattc tgctgcgtcc cgtagacgag
    12781 ctggaactca ccgtgcgctc ggccaactgc ctcaaggcgg agaacatcta ttacatcggc
    12841 gacctcatcc agcgcaccga gaacgagctg ctcaagacgc ccaacctcgg ccgcaagtcg
    12901 ctcaacgaga tcaaggaagt tctggcttcg cgcggtctga cactgggcat gaagctcgag
    12961 aactggccgc ccgccggctt ggacaagcgt taagctataa ttttcggctc ttttgaaaga
    13021 gccagtgcct cgggcagtac ctgatacggc tgcccgattt aatttactga taaggaaagc
    13081 accatgcgcc acggacacgg cctccgtaaa ctcaaccgca ccagctcgca ccgcctcgcg
    13141 atgctgcaga acatgatgaa ctcgctcatc gagcacgagg ccatcaagac caccgttccc
    13201 aaggccaagg agctgcgccg tgtgatcgaa cccatgatca ccctggccaa ggaagactcc
    13261 gttgccaatc gccgcttggc cttcaaccgc ctgcgcgatc gcgatagcgt gaccaagttg
    13321 ttcaacgacc tgggcccgcg cttcaagacc cgtcccggtg gctacacccg cattctgaag
    13381 atgggcttcc gcgttggcga caatgcaccc atggcgtttg ttgagctcgt cgatcgtccc
    13441 gaagttgcag acgagaccaa caacgaagcc taaaaatagg ttataatttc tgtctaccgc
    13501 gcgatggagc agtctggtag ctcgttgggc tcataaccca aaggtcggag gttcaaatcc
    13561 ttctcgcgca accagtcaga gaaaaggggt tacgacatcg tcgtagcccc ttttttcgtt
    13621 tgggcgcatt ccgcagcggt tgctacgtta caaggactgc ctggcatggc tgacttctct
    13681 cgctctctgg atctgtcggg agcttccaat tttcgcgatc ttggcggcta tgccagccag
    13741 gacgggcgcc agattcgttg gcgccggatt tttcgttctg accaccttgc cagtctgaca
    13801 ccgcaggaca ggcagttgct ggccggtttg ggcgtggcgc gtgcggtgga tttccgtggg
    13861 caggcggaga gcgctgcact gtcctatgaa ctgccgcatg tacgctatca agcgctaccc
    13921 atcgagccca tcgtggtgca gcgcgccaag gaaatggccc tggctggcca ttccatgacc
    13981 gcgccaatag cggtgcggct gatgcaggac acctatcgcg cctttgtgtc ggacaacacc
    14041 agccagtttg caagcctgtt cgagcagttg ctgctcgagg acacgccgtt ggtattccat
    14101 tgcaccgcag gcaaggatcg caccggcttc gcagccgccc tggttttgct tgccctgggg
    14161 gtatcccggg aagtggtgat gcaggactac ctgctgacca acggcctgta caggcgcccg
    14221 gctggtcttg tcagttcagc gcccgaggag gtgttgaacg tgatttggcg cgtgcaggcg
    14281 gagtttctgc aggccgcgct gcaggctgtg gatcatgacc atggtggtgt gcagcggtac
    14341 ctgaagcatc ggctggggat gcacgaggca gcgaccaagc gtctggcgga gctctatctg
    14401 cagccacatg ctgcttgacg cgcccatgaa aaaagcagcc catgggctgc ttttcggtca
    14461 aggcatggca tcgatcagag cgtcgcgtcc ttgagcttct tcagggcgcg ggtcttgatc
    14521 ttgaccgatg cgggcttggc cgggaaccag cgctcttcgc ccgtgaaggg gtctttgccg
    14581 aagcgtttct ttttcgccgg cacctgcaac aggccgatct tcagcaggcc gggcagggtg
    14641 aactccccgc aacccttttt gtgcacggcg cccaggatgg cggtttccag cgctgccagc
    14701 acggccttgg ccgtcttggg ctcgacgcct gcctgcgagg cgaggtgggc caccagcgtg
    14761 gttttgttga acggatcctt tacgggcttg ggtgccgcag gcgccgcggt cttcttcgcg
    14821 gctgcggtct tggcaggttc tgcagccttc ttggcgctgg cagtggtctt ttttaccggg
    14881 gcggtggttt tctttgcagt tgccatggtg ttggatggtt ttggaatgtc tgaatgcatg
    14941 ccagccaggc cggcgagtgg attctaggtg gtacaggcgt gcgcgatacc atgggtttgc
    15001 gcgcggttgc ccgcgaaagc tgcgtcaggc cgaccgttgg tgcattgttt gcaatgtacc
    15061 cgggcggtgg gcccgaggga tatgccagga ctgggcatta gcggtaagct ctcggccacg
    15121 tatttcacgt gatgcatagg agctgccatg agcgacacaa cccccttcgg tttcgggcgc
    15181 ttcgtcccgg gctttgattt tctgcagaac ctggccaaag gcgcggccgc tggcatgcct
    15241 gccatgccgg cggcgctgtc gggctgggtg gcaccgacga tgagcgtgga ggagctggac
    15301 aagcgcatac aggagctcaa ggcggtgcag ttctggctgg agcagaacgc ccgcgccatc
    15361 acggcaacgg tgcaggcact ggaggtgcag aagatgaccc tggccacgct gcagggcatg
    15421 aatgtggcca tgggagatat ggtgggtgcc tttggtgccg cgcaaccgtt caaggcaccg
    15481 ccccaagcca gcgccgcacc cgagccacag cccgcgccag cggcaccggc gccagccgca
    15541 gcgcccaaga gtgcggaagc cgcatcgcct caggccgacg cggcgcaggc cgaggccaag
    15601 gcgccgggca tggtcgatcc gatgcaatgg tggggagcgt tgaccaacca gtttcagcag
    15661 ttggcggcca atgccatgaa ggacgcgggc acgcaccatg cagccttcga ggccacacgt
    15721 gagatggcga gcggggcgct gaaaacagcc accgacatgg cagcgaagat ggccacccag
    15781 ggcatgccgg gcgccgtagc cgccaaaccc aaggcccggg cggcagcgcc cgcggcgaca
    15841 cccgaggctg cggccaagcc ggctgccaag ccacgcgccg catcgaaggc ggcagccacc
    15901 aagaaggccg cggcgccgcg ctgaaacggc caggctgcgg catccgtggc cttgcgcatt
    15961 ccccggggtc atcgatgaag ctctttccca acgcccatgc cacccacccg caatggcgca
    16021 tggcggcggc cctagtgttg gctcagctgc aggcccagat ggcccagccg caatatgcgc
    16081 gcaagccctc gctgggcctg ctctacatca ccgatcacta tgtggcggac gccgttgcgc
    16141 tgctgcgcta tctgggtcag caactgccct cggtggccga ttggagcggt accgtgggtg
    16201 tgggcgtagc cggcaacaat gtggagtact tcgacgaacc tgcgctgtcg atcatgttgt
    16261 gcgacctgcc acccgaacag taccgcgtgt tttccggtgt ggcgccacta cctctggggg
    16321 catctgcttt ttccgcacac acggccctgg tgcatgccga tggccgcacc cccgacctgg
    16381 ccgagttgct ggtggaaatg gcgggccgca ccagcagcgg ctacctgttt ggcggcgtgg
    16441 ctgccagccg tggcgccagc ctggcgtttg cgcagcatgc cggcggcgat ggcgccgcga
    16501 gtggagtgct gtcggggggg ctgtccgggg tggcgttttc cgaccaggtg ggcctggtgt
    16561 cgcgtgtcac gcaggactgc cagcccattg gcccacaagc cgtcgtgacc caggcgcagg
    16621 acaacgtggt gctgcgcctg gatggccagc ccgcgctgga cgtgctgttg cagactctgg
    16681 gtgtggagct caatggcgac ccgcagcccg ccttgcgcgc ggtgcgccac acgctggcgg
    16741 gcctgtcgaa tgcggatgcg ccggcgttgc agggcacggg gcattttggt gccgatacgc
    16801 gcgtgcgcca tatcgtcggg ctggatgtgg cgcgcggtgg cgtggcgctg gccgatcggg
    16861 tgcatgaggg catgcaccta gcgttctgcc agcgtcacat ggcggccgcg cgtgccgacc
    16921 tgacgcgcat ttgcgccgag atccgtgaag agcttgcgcc cgagtccccg gcgttgccgg
    16981 atgagcaggc ggcgacgcag cggcgcatct gcggcgcgct gtatgtcagc tgctccggcc
    17041 gcggcgggcg acattttggc gggcccagtg ccgagctgca aaccgtgcac cgtgcgctgg
    17101 gcgacgtgcc cctggtgggg ttctttgcct cgggcgaaat tgccgcgcat tggctttatg
    17161 gctacaccgg cctgctgacg gtgttcgtgg cgcctgccga cacggcgtga acctgcccgc
    17221 ctgccgttgg ctgggtggcc gatgggcgtg tcaagggtac acggcttcgc ccaggttgag
    17281 catcaggcgg ttggcccagg caaacagggc cacagcatgc acggtatcca gtatctccag
    17341 atcgctcagg ccggcgtcgc gcagcgccgc caggtggtgc gcgccgaagc tgccgggcgc
    17401 gcgcgtcagg gccgcgctgg cctgcacgat ggcgcgctcg cgcgcattcg tacccgcctg
    17461 atcggggtcg atgaagacct gtgcgatgac gtcattgcgc ttggccagtt gttcaaaacg
    17521 ctgggcgtgc accgaagcgc agtagacgca gccgttgata cgcgagacca cggcggtggc
    17581 cagttcgcgt tcggatcgcg gcaggccgcc gggtgcatac atgatggcgt tgaaggcctc
    17641 ggagcgctcg gccagcacgc gcggctggtg caccagcagc aggtagaagt cggatacgcg
    17701 cgccttcggg tggctggcgt ccagcacggc gttctgttcg gcgctggcct gggtcgcgtc
    17761 caccaccggc agccaggcct tccagtccag tgtctcgctg gtgtagccgt tcaggcgcag
    17821 tggttcgccc ggcgcgggca gattcgccgg gtggatgaag agcgcctcgg cggcgggcgc
    17881 ggccggggtc gcagtgctgc ctgccgggcc cagcgcagcc agtgctgcca cgcccgccac
    17941 caggcgcgcc tggaaggccg caaagcctat gagctgtgcc agcaacacca cgtctgcggt
    18001 ggtcaggccc gcggcgggca gcgccagcag cgcggcccgg tcggcagccg cgggctgcgt
    18061 ggccagcagc tgcgtgaagt ggtgcatggc ctgccagcgg gcatctggcg tggccgtggg
    18121 caggcgctgc agctgtgctg cgtagtgctc gcccagggcc gtcacgccgc tggcggcgga
    18181 cacggtctgg gccacggcca ggcgctgcgc cagcggcagg ccgccgggta ggtcgggcgc
    18241 cagcagcagg tcttcgcagg cctgggtggc gctgatggct ttggcgcgct gctggcgcgt
    18301 ggcctgggtc tggggtgtgt cttgcaggtg ggtgagccgg gcgatggtgt cggtcatgtc
    18361 ggtggcgatt cgggcagtac gcgttggcgg gtgtcggcgg gcgtccattc gtcgcccagc
    18421 agttcgggtt cggcataggc ctgcagggcg gcgaaatgct gctccacatc ctcggcatac
    18481 aacagaccgg cgatgccgct ggccaggcgt ctggcgccat cgctgatggc ggggatatcg
    18541 cccgacaccg tgccgtgcga cagcgccgcc gggtagcaaa agcagtggat gcgatcgagg
    18601 cccggacagg caccggggga cttctcgcgc agctcgaaca ccgggcccag gtcgggcgag
    18661 tcgaacagct cgcggtcgtc ctcgcccggc gggctctggt agcgctcgcc ccagctgcgc
    18721 acatgcggcg cgaaggcggc gaactcgggc ttttgtgccc agtcgatggc aaagccagtg
    18781 gccaggatca ggaagtcgaa ggcgaatcgg ccctgcgatg tggcgacctg cagcgcctga
    18841 ccctgctgcg tgacctgctg caccgcgcag ccgaagtgga agaacgcgtg cgcatgccgc
    18901 gacacgcgca gcgtgctgcc acgcggtggc ggcacctgct gcacgttgat gtagtggcgt
    18961 atgcgccatt tccaggcatc gggcagctcg tggtggccct gcgtcaggcc gggcaagccc
    19021 gagcccttgg acttgttgat ggtgggcatg gcggtgcggc gtgcgatcag atccacgctg
    19081 gccgcgccgc attccagcgc cgtgcccgcg ctgtccatgg ccgatgagcc catgccgatc
    19141 acgcccacgc gtttgcctgc cagcgtggcg tagtccagca catcgctgga atgcgcccac
    19201 aaaccaccct tgctggcgtg ccccacgccg tccatgaagg ccggtatctg cgccccgccc
    19261 aggccgtcgc ggccggtggc cagcaccacg cggcgcgcca gccaggtggc gctgcaggtc
    19321 ggtgtgtgca catccacctc caccagcgcg tcggcgcgcg ggcgtacggc cgtcaccgcg
    19381 tgttcattgc gcacgtcggc ctgcgtcacg cggcggtacc agcgcaggta gtccatccac
    19441 tgcaggcgcg ggatcttgtc cagcgcgtcc caggcggacc gaccccactg cgcctcgaac
    19501 caggcgcgga aggtcagcga gggcaggcct agcgccgggc cggtcagctc cttgggcgag
    19561 cgcagcgtct ccatgcgcgc cgtggtggcc caggggccct cgtagtcggt ggcggcgcgg
    19621 tccagcagca cggcggcaat gccctgctgt gccagtgcca tgctggccgc cagtccggcc
    19681 tggccggcgc cgatgatgag gacgtcgatc accggctggc cgccatggct gagtgcgggc
    19741 agccaggggc gcgcgcgcca gttcagggtc agcaggtcat gcgccaggcg ttcttcaagg
    19801 gcggccagtc cggccgcgcc cagggcgggg gtgtcaggca tggggtgcgg agcgggcggc
    19861 atcagttgat gctgattttg gcgtccttga ccacctgggc ccactttgca cgatccttgc
    19921 gcacggtctg gcggaactgc tcgggcgttt ctatgcgcac ggtgttgccc tgggactcaa
    19981 agcgctggcg gatttctggc agctccagca cctggcgcaa tgccgtgttg agcttgttga
    20041 ccacggtggg gtcggtgccc ttgggtgcaa agatgccaaa ccagatcgag ctgtcgaagc
    20101 cctttgtgcc gggcaggccg ctggcggcaa tggtgggcac ttccttcacg gcgtccacgg
    20161 gcttggccgt ggtgatgccc agcaggcgga ccttgccgct cttgtagtgc gccagcacgg
    20221 tctgcacctg gttcatgatg cagcaggtct cgccgcgcag caccgaggtg atggcctcgg
    20281 gcccgccctt gtagggaaca tgcaccatgt ccaggcccag gcgcgcattg aactcggcaa
    20341 acgccatgtg cgtgccggcg ccattgccgg tgctggcgta gttgtacttg cccgggttgg
    20401 ccttgacgat ctcgacgaac tccttgaggt tgttggcgtc gatcacgctg gggttgatgg
    20461 tcagcacgtt ggacacgtcg atcagcgggc ccacgggcac gaagtcggcc tccacgtcga
    20521 agggcaggct cttgtacagc gcggcgttgc tgccgtgcgt ggcggccgtg ccgaaggcca
    20581 gcgtgtagcc gtcgggcttg gcatgggcca cgtattcgct ggcgatgttg ccggccgcgc
    20641 cggacttgta gtcgatgacg atgggctggc ccagcttctg gcccaggggt tcctgcaccg
    20701 cgcgcgccac cacatccacg cctgagccgg ccggaaagcc catgatcagc gtgatgggct
    20761 gtttgggcca gccgttggct tgcgccagtg cggcgctgga ggcgaagagc agggcggttg
    20821 cggcaagcgc agtgcggcga aggatggggt gcatggcggt cttctttgat gcgtgtcagt
    20881 gcaatgatcg tcgaaatcac ctaagcgcgt tcgacgttcg gcattgtgcg caaaaccaca
    20941 tgcaggggcg ccataagctt atggcgcctc atgaccgcgc cacccttcaa tgccgatggc
    21001 tgccgccggg gcgcgggccc tgtggcgccg gccggccacc atggccccag ccgccgccgg
    21061 tgaaatgcca ctgcccctgt cgttgctccc agcctggctg gcggtaatgc tggccgtggc
    21121 gcgcctgcac ccaatggccc ggcacccagg catagcgctg gccgcgccat tgccaatacc
    21181 cctgcgacca ggccatgcca cggcgtgcgg ccggtgcgcg ttcatagcgc ggcggcggcg
    21241 gggctgggcg gtagtgcgcg ggctgcgcgg cgtgggtctg atgctgtggc acgccgggcg
    21301 cggcatgggc ggcgccggcc agggccagca gtgcagtcag cgtggctgcg gcaagcccga
    21361 gggggcgggc caatgcgtgg gtcagggggt aggccatggt gttctccttg cagattgcgg
    21421 ccggattggc cgcgggagac tccatgctgc ccgccttgtg tcaacgccac ggaagcctgc
    21481 atggctggcg cgcaaagctc tgtgtgaatt tgtgaaaaat tgggctgtag cgctttctgg
    21541 ttaagcgctg acagctatca atataggaat atctgccaac ctgcctggcg cccccggcag
    21601 ctcgtccttg gcccgcatgt ttgccgcccg gcggcgggtt tttgcgcaca atgcgcggca
    21661 cttccacagc agcaccccca tgagcgcccc ccatcccaac cagtttgcgc tgctcggcca
    21721 gcgtcgcttt gcaccgtttt tctggaccca ggccgccggc gccgccaacg acaacctgtt
    21781 caagttcgcc ttcaccgtga tggttaccta ccagctcggc gtgtcctggc tgccgccgtc
    21841 cctggcgggg ctggtgatag gggcgctgtt catactgccg ttcttgctgt tctcggccac
    21901 ggccgggcag atcaccgaca agctggagaa gacgcgcatc atccgcttcg tgaagaacct
    21961 ggagatcgcc atcatgctgc tggcggctct gggcttcatg gccggccatg ccgcgctgtt
    22021 gctggcctgc gtgtttctga tgggcctgca ttccacgctg ttcgggccgg tcaagttcgc
    22081 ctatctgccg caggtcttga gcgagcgcga gctcaccggc ggcaatggca tggtggagat
    22141 gggcaccttc gtcgccatct tgctgggcaa tgtggccggc ggcctgctgg tggccatgcc
    22201 cggcgtgggt catggcgccg tcgccctggc ctgcctgggc ctggcgctgg cagggcggct
    22261 ggcggcgcag ttcattcccg gcgcgccggc caccgacccg ggactggtgg tcaactggaa
    22321 cccggtgagc gagacctggc gcaacctcca gctggcgcac ggcaacatcg tcgtcttccg
    22381 ctcgctgctg ggcatctcgt ggatgtggtt cttcggcgcg gtgttcctct cccagttccc
    22441 cagctttgcc aaggatgtgc tgcgcggcga cgagcaggtg gcgtcggccc tgctggtggt
    22501 gttctcgctg ggcataggca cgggcgcgct gctgtgcgag gtcttgagcc ggcgccatat
    22561 cgagattggc ctggtgccgc tgggggcgat tggcatgagc gtgttcgctg gtgacctgta
    22621 ctttgcctcg cgcggcctgc cgccttcggg tgtcatggcc ctgggcgcct ttctgacgca
    22681 gcccgcgtac tggcgcgtga tgctggacct gttcttgctg agcctgttca ccggcctgta
    22741 tagcgtgccc atgtacgcgc tgatccagat gcgcagccag gccacgcacc gcgcgcgcat
    22801 catcgccgcc aacaacatcc tgaacgcgct gttcatgatc gtcagcgccg ttctggccgg
    22861 gctgctgctg caggcgggct tcacggtgcc gcagattttt ctgttcacgg ctatcgccaa
    22921 cgccgtggtg gcggcctaca tctttttgct ggtgcccgaa tacctgctgc gcttcgtggc
    22981 ctgggtgctg tcgcgctgcg tgtaccgctt tcgcgtgcgc ggcgatgagc acatccccac
    23041 ggcgggcgcg gccatcctgg cctgcaacca tgtgagcttt gtcgatgccg tgctgctgat
    23101 ggccgccagc ccgcggccca tctacttcct gatggaccac cgcatcttcc gcgtgccggt
    23161 gctgggcaga ttgttccgcc tggccaaggc catccccgtg gcgccgcaga aggacgaccc
    23221 cgccacctac caggccgcgt tcgagcgcgc cgcccaggta ctgaaggagg gcgacctgct
    23281 ggccatcttc cccgagggcg gcatcaccag ggacgggcag ttgcaggagt tcaagggcgg
    23341 catcatgaag atcatcgagc gcgcccaggc cgacggcgtg caggcgcctg tggtgcccat
    23401 ggcgctgacc aacctgtggg gctctttctt cagccgcatc gagcagggcg gggccatggt
    23461 gcggccgttt cgccgcggca tgttcacgcg cgtgggcctg aacgtgggcg cgcccctggc
    23521 ggcggccgag gtgcggcccg agccgctgcg cgcgcgcgtg gccggcctgc tggcgctggg
    23581 gtgacagttc tcagccgccc gcacgcatgg cctgctcggc cagatagcgc gccgtggcgt
    23641 cgcgcgccat cgcggccgaa cccagcacct ggccggccgc gtccttgacc accgagaagc
    23701 tcatgtccac atacagcctg cgcccgtcct ggtgcaggcc gcgcgtggtg cgcacctcgg
    23761 ccgggcggct gcaggcgccg cgctgcatgg cgcgctcaaa gccgcgccag tgcgcctcgc
    23821 gcaggtgcgg cgggatgatc atgtccacgc tctggcccag ggtctggacg gcggcaaagc
    23881 cgaacagcgc ctcggcaccg gcgttccagg cacggatcac gcccgcgcgg tcgatgaaga
    23941 tcagcgcatc ggccacatcg gccacgatac gctcccaggg cggcggggga aggcggtctg
    24001 tgctcatggc gggcattgtc cgcgcgggtg cgccactggc aagcgcgggc tcttgggttt
    24061 gagtgaaaac agccagtagc acccttgtat attgcgctga tagctattaa atttgtagtt
    24121 aaatgaccgg agggcaacat gggcaaggca ttgcgtctgt ctgaaaaatg gtttcgccgc
    24181 ggcctgtggc tggtggcgct ggtgttcgcc ggctttctga tcggcctggg cggcaccatc
    24241 gttggcgacc tgccccaggt ggagcagccg ctgctgctgg acgactttct ggatagcggc
    24301 gcggccacgg cgctgcgcgg ccagattcag caagcgcaac aggccgagca ggacgcccag
    24361 gccgcgctgg agcaggcgcg cctgcgccac gcccgggcgc gcagcgccac ccaggccgag
    24421 cgcgagaccc tggccaactg gctggccgcg cgcagcgcca cccagcgcgc cgagcatgac
    24481 cccgaggtca tcgcccgcac ccaggcgctg gacgccctca aggccaccga gctgcaggcc
    24541 cagcaggccg tgcaggcgca agaccaggcc ctgctggacg cgcgccaggg cgggcaggcc
    24601 gcgcagcagc ggctgaacgc catggagcgc gatgcccacg acaagctggc cgccgagcgc
    24661 cggcgcgtcg agctgcgcgt gtttctgtac cgcctggcgc tcacgctgcc gctgctggcc
    24721 gtcgccggct ggctgtttgc caagaaacgc cagggcacct ggtggccctt tgtctggggc
    24781 ttcatcttct ttgcgctgtt cgccttcttc gtcgaactgg tgccctacct gcccagctac
    24841 ggcggctatg tgcgctacat cgtcggcata gcgctcacgg cgctggtggg gcgccaggcc
    24901 atcgtcgcct tgaaccgcta cctggagcgg caaaggcgcg ccgaggccat gcccgccccg
    24961 cagcggcgcc agggcttggg ctacgacctg gccctggcgc gcctggcgaa aaacgtctgc
    25021 cccggctgcg agcgcccggt ggatttgaag gacggcaaga ccgacttctg cccgcattgc
    25081 ggcatcggcc tgttcgacca ttgcgggcac tgcaacgcgc gcaagagcgc ctttgccagg
    25141 ttctgccagg cctgcggcca ggcggcgggc ggcgttgccg gcgggtatga agccccatag
    25201 gccggcttgg cgcggccagg ccaggcagct gcgtaaaaca tcgataaaac tgcctatagc
    25261 gcttatggaa taagcgtgta tagctatgaa aattcataac ccccacgggg gtgggcggcg
    25321 ctgcggcgcg gcaggttttc agccctggtc cgcccaatgc ccgcgcacct ggtcgcgccc
    25381 cagtgccttg gcctggtaga gcgcggcgtc ggcccaggcc agcaggcgct ccaggctgtg
    25441 ctgctggtcg ggcggctggg tggcgtgcag gccgacgctg agcgtgacct gcagcgcgct
    25501 gccgtcctgc agcctgaacg ggtgttcgcg cagctggctg cgcaggcgct cgccgatcag
    25561 ctgggcgtgg ctggctgcgg tggccggcag gatcagcgcg aactcctcgc cgcctatgcg
    25621 cccgaacagg tcctgcgggc gcaggttgcg ctgcaccagg gcggcgaact cgcgcagcac
    25681 ggtgtcgccc tgggcgtggc catggctgtc gttgatgcgc ttgaagtggt ccagatccat
    25741 cagcagcacg gccacgggcg cgccctgctg gtgcaggcgc tgcaccaggc gggcgccctg
    25801 ctccatcagc ttgcgccgcg acagggcgcc ggtcagatgg tcgtgatcca cggcctgctg
    25861 caactggctc agggtctgcg agcgcagctg gtgcgccacc gccacggcca gtggcgccag
    25921 tgacaagagg gccatcccca ggcggaatga ggtcacggcg gccagctgat cgggcaagaa
    25981 gctgatggtg tccagggcgg ccacggccgt cttccagctg cacaccagca ggttcagcac
    26041 cgtgatgggg aacaccccgt aggccatggc gcaccacacc agcgcgggca ccgcaaagcc
    26101 cagcatgccg ggcccgttga caaagtaggc cagggcctcg caggccagca gggccagcag
    26161 gggcgccaca cctgcctgcc gcagcggtcg cagcagccgc gcccagtccc actgccagaa
    26221 ccagccccgg ggcgcggcca gcaccagggg caggaagagc agcaggttgt acatctcgga
    26281 cgacagccac atcagcatgt ccgtccacgc ctgggatgcg aacgcccatt gcatcaccgg
    26341 ggtggccggc agcgcgctgc ccagggcggc cacgctgctg ccggccagca gggtcaatac
    26401 ggcgcgctga cgcgtgaagc cgacgatgct tgccgcatac ggcctgaaga acagccagcc
    26461 caccgccacg cccagcaggt tggcgccgtt catgcccagg gcgcgcagcc agggcgcacc
    26521 cgtcagcatg tcggcggtca ggtagccgac cagcgcgcat atccaggccg tggggccgcg
    26581 tgcccaggac gggttgcgca gcagcagccc cagcagcacg gcattggcgg gccagatggc
    26641 ggcggaatac cccgccgggc ggctggctat gcccagcagg caggcggcga acacgctggc
    26701 cagcagcaac agcggatcgc gccagggggc ggcggactgc tgcgtggcga tggggggcgt
    26761 gctcatgtag ggggcgcttg gccttggcaa aaagttgaca gtgtccgtgg gttattgacg
    26821 ccggggtgtc agcaagtgca aatcatgttc aattggtgca gggctaaacg ccacgcgccc
    26881 ggtcggcctt gaagcgcgcg cggaactcgg caaagcgacc ggcgtccagc gcgtcgcgta
    26941 tctcctgcat cagattcagg tagtagtgca ggttgtggat ggtggtgagc atggggccca
    27001 gcatctcgcc gcagcgatcg aggtggtgca ggtaggcgcg cgagaaaccg ccgcgcccgc
    27061 cgtcctgcca ggccacgccc tcgctgcccg cgcaggcgtg gcagcggcag ctcgcgtcca
    27121 gcggctggtg gtcgctcttg tggcgcgcat tgcggatctt caggtcgcca tggcgcgtga
    27181 acagcgtgcc gttgcgcgca ttgcgcgtgg gcatgacgca gtcgaacatg tccacgccgc
    27241 aggccacgcc ctccaccaga tcctcgggcg tgcccacgcc catcaggtag cgcggcttgt
    27301 gctggggcag gcggtgcggc gtgtgcgcca tgatggccag catctcttcc ttgggctcgc
    27361 ccacgctcac gccgccgatg gcgtagccgg ggaagtccat ctccaccagg gcgtccagcg
    27421 actcctggcg caggtgcggg aacatgccgc cctggacgat gccgaacagc gcgttcgggt
    27481 tggccaggcg ctcgaactcg gtcttggaac gcagggccca gcgccggctc atctccatgg
    27541 atttgcgcgc ctcggcttcg gtggtcttgt ggccattcgt ctcatacggc gtgcactcgt
    27601 cgagctgcat cacgatgtcg gagttcagga tggtctggat ctgcatgctg acctcgggcg
    27661 acatgaagag cttgtcgccg ttgaccgggc tggcaaaggt cacgccctct tcggtgatct
    27721 tgcgcatggc gcctaagctc cagacctgga agccccccga gtcggtgagt atgggcttgt
    27781 cccactgctc aaagcggtgc aggccgccaa agctttggac gatgtccaac cccgggcgca
    27841 tccacaggtg gaaggtgttg cccaggatga tttgcgcgcc catctcatgc aggctgcgtg
    27901 gcatcactcc cttgacggtg ccataggtgc ccacgggcat gaagatgggg gtttgcacca
    27961 cgccgtggtt cagcgtgagg cggccacggc gggcgtggct ggaggggtcg gttttcagca
    28021 ggtcgaattg cagcatgggc ggcattttcc cagaccctgc tggcctacac tggcagccac
    28081 agcacaagga ggacgcatgt tcagaatcat gattgccgtt gatggctcgg agctggcgct
    28141 ggacgccgtg cgccatggca tcgccctcgt gcgccgcggc ggcctgc
//
LOCUS       DAOMTQ010000017        27534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_157_length_27534_cov_27.663488, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000017 DAOMTQ010000000
VERSION     DAOMTQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 27534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27534
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157_length_27534_cov_27.663488"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="PK518_03175"
     CDS             complement(<1..131)
                     /locus_tag="PK518_03175"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806625.1"
                     /note="converts protoheme IX and farnesyl diphosphate to
                     heme O; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HRM47940.1"
                     /translation="MAPTPSPAPAPAGPFGMAGAGALPSRLAQFYALTKPRVVQLIV"
     gene            complement(134..1306)
                     /locus_tag="PK518_03180"
     CDS             complement(134..1306)
                     /locus_tag="PK518_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRM47941.1"
                     /translation="MNDTQALYDLAPMLEVMALGLVIALGPLAWVWWRSRGSTPVRRL
                     QALTVLTLFLTFDLVMFGAFTRLTDSGLGCPDWPGCYGSASPVGAHTEISAAQQAMPT
                     GPVTHGKAWVEMIHRYLATGVGVLIIALTVGAWRQRGANRVLSPWWPTATLVWVCLQG
                     AFGALTVTMKLFPAIVTLHLIGGLVLLALLCLQAVRQTQHTKGVAPVALPGGLRWLLA
                     GAMALVALQSVLGGWVSTNYAVLACTTFPQCQGSWWPEMAFSEGFQIWRELGHLQDGS
                     LIGFAALTAIHYVHRLAAYGVLLVLALLVWRLLRAQLLRDQARWLAGLGALQLLTGLS
                     NVILDWPLVAAVLHTGGAAALVVVLTWALVASRSATAEARAAHVQARQPAAKRVSA"
     gene            complement(1303..2001)
                     /locus_tag="PK518_03185"
     CDS             complement(1303..2001)
                     /locus_tag="PK518_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47942.1"
                     /translation="MSGSNLSDVYPPLAASQTPEPTGQRPQRARRIGRWQLLGVLLVC
                     AAPVIASYFTYYVIRPEGRRNFGELVEPQRPMPAEALVVALDGQPRKLGELRDQWLLV
                     SVGAAACDADCRNNLYLQRQMREAMGREKERLDWVWLVSDDAALPADIAPALEQATVL
                     RADDAMLAQWLAPQAGHGLSEHLYVVDPQGQWMMRFPARMDGAGATQARRDLERLLRA
                     SASWDQPGRAKEQP"
     gene            complement(1943..2719)
                     /locus_tag="PK518_03190"
     CDS             complement(1943..2719)
                     /locus_tag="PK518_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRM47943.1"
                     /translation="MDPIISPAQARFGWRFWLLTLAALVAMAVTATLGAWQLSRAAQK
                     QALQAMLVAREQMPVLVGRDLERARVGGVAALLQRNVQLQGQWVPQATVFLDNRQMQG
                     RPGFWVLTPLRLPGEQGVVLVQRGWAPRDFQERTRLPDVVTPAGNVQIHGHVAAAVAR
                     LYEFAPAGEGEGASRIRQNLALAAYGAETGLPLLPLVVVQTGGASDGLQRDWAPADSG
                     VDKHYGYAFQWFGLSGLVFILYVWFQFVRRLPSLGGKSDA"
     gene            2709..2942
                     /locus_tag="PK518_03195"
     CDS             2709..2942
                     /locus_tag="PK518_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRM47944.1"
                     /translation="MGSMKILVALAFLAIIASLGSALFFMMRGDGNDRQRSKHMARAL
                     ALRVGLSIVLFLCLLLAWKLGYIQPTGLPAVMR"
     gene            complement(2988..3872)
                     /locus_tag="PK518_03200"
     CDS             complement(2988..3872)
                     /locus_tag="PK518_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRM47945.1"
                     /translation="MSASIQGAKPPYYFVPAQSSHPVLVAVGLFFVILGAAMWINGHD
                     WGKYSFAAGLLWWLVVLFRWFREAAHESESGLYGRKVDVSFRWSMSWFIFSEVMFFGA
                     FFTALWWARAHSVPALGSLENALLWPEFKAAWPSVVAGATASPANVVDPFQTVGPFWL
                     PTINTALLLSSGVTLTIAHHALREDQRSKAIGFMWITVLLGVTFLCVQAYEYFHLYTD
                     LNLKLSSGIYGSTFFMLTGFHGFHVFVGMLMLFFITLRLQNGHFTAERHFGFEGAAWY
                     WHFVDVVWLGLYILVYWM"
     gene            complement(3926..4141)
                     /locus_tag="PK518_03205"
     CDS             complement(3926..4141)
                     /locus_tag="PK518_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HRM47946.1"
                     /translation="MTEAGDRPPARPSASLWRTMRTIAWALFGVRNADRHRQDQESLR
                     PLQVLGMGLLALVLLVLLLMALVNWLV"
     gene            complement(4138..4758)
                     /locus_tag="PK518_03210"
     CDS             complement(4138..4758)
                     /locus_tag="PK518_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRM47947.1"
                     /translation="MARRSDNLRMLGKLLVVTLGMFAFGYLLIPAYKAICEATGVNIL
                     SLTERQVPGNGVAADEARALVKNTQVDTTRTITVEFDANARGPWQFKPAKRSMQVHPG
                     EMATVLYEFENVQDRRMSAQAIPSYAPRQAAPHFNKLECFCFSQYTLDPGEKKEWPVV
                     FVIDPRLPKDVTTITLSYTFFEVGGKIPSAPQSTAGAVQTPGAGAS"
     gene            complement(4773..4889)
                     /locus_tag="PK518_03215"
     CDS             complement(4773..4889)
                     /locus_tag="PK518_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HRM47948.1"
                     /translation="MTSQEQRKRNARLGLMLAAVALALFVGFIVQRYFVLGH"
     gene            complement(4917..6542)
                     /gene="ctaD"
                     /locus_tag="PK518_03220"
     CDS             complement(4917..6542)
                     /gene="ctaD"
                     /locus_tag="PK518_03220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRM47949.1"
                     /translation="MSAVLDNHGHAAGHDDHHHHQPTGWRRWVYATNHKDIGTLYLLF
                     SFTMLMIGGVLAMLIRAELFQPGLQIVNPEMFNQFTTMHGLIMVFGAIMPAFVGFANW
                     MIPLQIGASDMAFARMNNLSFWLLVPAGIMLAASFFMAGGAPAGGWTLYAPLSAQMPM
                     SLDAAIFALHIMGASSIMGAINIIVTILNMRAPGMTLMKMPIFCWAWLITAYLLIAVM
                     PVFAGAITMTLTDRHFGTSFFNPAGGGDPVMYQHIFWFFGHPEVYIMILPAFGMISQI
                     VPTFARKKLFGYTSMVYAIAAIATLSMIVWAHHMFTTGMPVTGQLFFMYGTMLIAIPT
                     GVKVFNWTATMWRGSMTFETPMLWAVGFIFVFTIGGFTGVIPAVVPVDTQIQDTYYII
                     AHFHYVLVAGSLFAIYGAYYYWSPKWCGVMYNETRGKIHFWWSMISFNVTFFPMHFLG
                     LAGMPRRYADYPMQFADFNMIASIGGFFFGAAQVYFFFAVVLPMLRGKGDKAPQRPWE
                     GAEGLEWEVPSPAPFHTFEEPPKLDATATRVIG"
     gene            complement(6580..7743)
                     /gene="coxB"
                     /locus_tag="PK518_03225"
     CDS             complement(6580..7743)
                     /gene="coxB"
                     /locus_tag="PK518_03225"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRM47950.1"
                     /translation="MTSISNKLASPLLVALASWGTLAHAVQDLPGGPAVNQLNLHPPV
                     TKIAEAQHELHWMLMIICTVIFIGVFGVMFYSIWHHRKSRGAKAANFHESTAVEITWT
                     VIPFLIVIAMAAPATKVIVAQKDTTNAELTIKVTGYQWKWGYDYLTGEGEGIGFLSTL
                     DSSQRALSSAGTPQGDNYLLKVDNPLVVPVNKKVRIITTANDVIHSWMVPSFGVKQDA
                     IPGFVRDTWFRAEQVGDYYGQCVELCGKEHAYMPIHVKVLPQVEYTAWVADQQNKAAA
                     RRDDPNKVWALADLQARGAKVYAANCAACHQANGKGAGPIKALDGSAIVKNANHAPQI
                     QVVLKGAGNGAMPSWAALSDTDLAAVVTYTKNAWSNKTGQLVQPAEIVALRAK"
     gene            complement(8215..9123)
                     /locus_tag="PK518_03230"
     CDS             complement(8215..9123)
                     /locus_tag="PK518_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HRM47951.1"
                     /translation="MSEQRPPTIDPVAAARWHAMVSDESPWLHEEVARRMQDRLQWMV
                     NAPTAWCDWDPLRGGMQGHALVSERFAQAACQIHETSSPQGEALARERLARRWWSPAR
                     WIANAPRFGQPEAASVQMLWSNMALHMTADPQALVTQWHRALAVDGYLMFSCLGPDTL
                     RELRALYAELDWPAPGHALTDMHDWGDMLVHAGFAEPIMDMERITLTFATPQRLLQEL
                     RGLGRNLHPQRFAALRGRNWRARLEQALAQRLADAGADGQLALTFEIIYGHAFKPAPR
                     VRLEGSSAVSLQDMRAMLHAGRSSTR"
     gene            9159..9887
                     /locus_tag="PK518_03235"
     CDS             9159..9887
                     /locus_tag="PK518_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRM47952.1"
                     /translation="MLDTRPAGIWRRGLATLAALGARLPSQCAVCHAWPAQRLCAACC
                     ARFAPRLARCATCACVVHGGVAQCGQCLLHPPPLDACLAAVDYGYPWSQLLAQFKFQD
                     DPGWATALAALMRQTPGAQELLEQADWVLPVPLAPGRLRHRGYNQALLLARRLAGAHV
                     RPDLLLRTREAEAQAQLTRAQRLRNLRGAFVLEPLRAQQLAGRRVLLVDDVMTTGATL
                     HAAAAPLREAGVAHVGALVLARTP"
     gene            9934..10407
                     /locus_tag="PK518_03240"
     CDS             9934..10407
                     /locus_tag="PK518_03240"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRM47953.1"
                     /translation="MFHIVLVEPEIPPNTGNVIRLAANTGCTLHLVEPLGFSMEDRLM
                     RRAGLDYHEYAKLRLHKGWTALLREMQPDPARMFALTTHGTRLVHDTGFLPGDWFLFG
                     AESRGLPPELRESFAPAQRLRLPMLAGQRSLNLSNAVAVTVFEAWRQNSFLAPQA"
     gene            complement(10412..11032)
                     /locus_tag="PK518_03245"
     CDS             complement(10412..11032)
                     /locus_tag="PK518_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRM47954.1"
                     /translation="MPVIAVASPKGGVGKSTLSTNIAGYFASRGHTVVLGDADRQQSA
                     RLWLQQRPATARPIGAWEVDADWISAPPKQATHAVLDTPAGLGGWRLKDALKLADRII
                     VPLQPSLFDIYATGQYLDELAAQRPGGLERVAIVGMRVDPRTIVAEKLRNFVDSLGLP
                     VLGYLRNTQNYVQLAAQGQSVFDVAGTRVARDVEQWQGICDWLDAV"
     gene            complement(11113..11751)
                     /locus_tag="PK518_03250"
     CDS             complement(11113..11751)
                     /locus_tag="PK518_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRM47955.1"
                     /translation="MDWHTWMAFFAASWVIAISPGSGAVLSMSHGLSYGVRKTGATIL
                     GLELGLLFILIVAGAGVGSLLIASELAFTVVKVLGACYLIYLGFSQWRAGSAGTLGGD
                     LLAAPASWRRRVLTGALTNATNPKGILFMVAVLPQFMSEARPLWSQLLVMALTLVSVD
                     LIVMNGYAASASVLRRWMQNASAMRAQNRVFGGLLMLVGAGLFFVKRGTQHQ"
     gene            11923..12615
                     /locus_tag="PK518_03255"
     CDS             11923..12615
                     /locus_tag="PK518_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47956.1"
                     /translation="MNRRIIVLSLLLSPLAHAQPERLVPSSPVDSVVLYVIPTDGIPE
                     QVAATVARTLTEDTGMWVKSALQVPSGVTEPFAGTNQYPAEDYLPLGATFAKRLPDAG
                     PRTYFIVLTDKDINSRSRNFRFQYSMHSPMARTSVLSVARLLFTRDGNPASGDVIRLR
                     IQKMLMRIVGEMKFGWQRTTDPADLMYSPIMSIDDIDRMSLTHTAQTRGIPRYDFQPS
                     ATEPPRPLPNHI"
     gene            12733..14601
                     /gene="kefC"
                     /locus_tag="PK518_03260"
     CDS             12733..14601
                     /gene="kefC"
                     /locus_tag="PK518_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HRM47957.1"
                     /translation="MAQAPVWLTYGFTYLAAAVIAVPIARALGLGAIIGYLAAGIAIG
                     PWGLALVSNVQDILHFAEFGVVLMLFLVGLELQPSRLWSLRRPILGLGMAQMAGCALL
                     LWGCAWALGLPWRVALVGALGLALSSTAIALQVLNERNLLRTDSGQKAFSILLFQDVA
                     AIPILALLPLLGVAARAEALQPHAPLDLLPEALKIIAVVAAIVFGGRWVLRPLLRWIA
                     KSKTPEIFTAASLFLVVGIAMLMLTVGLSMALGAFLAGVLLADSEYRRELETDIEPFK
                     GLLLGLFFIAVGMSIDFGVIAHHPFAMLGLLLGFLAIKAAVIWLLARVTAMAYQERPL
                     FTLLLAQGGEFAFVVFQSGATFGAIAPDHASLLIGAVALSMLMGPLLLVWLDRVLLQR
                     HATLQSAPQAQEISEPQSAPVIIAGFGRYGQIVARMMLAQGVPATVLDHSVEMLEVAH
                     TFGYRVFYGDATRVNLLRMAGAQQARILVVAVDAPEQSLKIVQLAKKHFPHLHIVARA
                     RDVTHWHALRDLGVQHVERELFESSLRTARTVLELTGLSQADADALAERFRAHNIALS
                     ERMYEHHNDREAMIAVARQGRQQLVEQLAKERQERMAAAEAGSGLTPDKPQPGKSS"
     gene            complement(14664..15644)
                     /locus_tag="PK518_03265"
     CDS             complement(14664..15644)
                     /locus_tag="PK518_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM47958.1"
                     /translation="MTPIRITRRSLLAGLAAAALPLGALAQNYPAKPVTIVVPFAPGG
                     TTDILARIVGQGLGTELGQPFVVDNRAGAGGNIGASLAAKAPADGYTLFMGTVGTHAI
                     NQALYKKMPFDPVKDFAPISRVATVPNLLVAHPSQPYKTVKELIAYAKANPGKVTYGS
                     PGSGASPHVSGELFKSMTGTDLLHVPYKGSAPAMTDLLGGQIAIMFDNMPSAIQHVRS
                     GKLRPIAVTTAKRSPELPDIPTIAEAGVPGYEAMSWFGLFAPAATPKPVLDRLNAALV
                     KVLNQPDVKKKIAEQGGDVVAETPEQFAAFIKAETAKWGKVVKDSGATVD"
     gene            complement(15679..16881)
                     /gene="pcaF"
                     /locus_tag="PK518_03270"
     CDS             complement(15679..16881)
                     /gene="pcaF"
                     /locus_tag="PK518_03270"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRM47959.1"
                     /translation="MTQAFICDAIRTPFGRYGGALAGVRTDDLGAIPIKALMERNPGV
                     DWKAVNDVLYGCANQAGEDNRNVARMAALLAGLPIAVPGGTINRLCGSGLDAVGTAAR
                     AIKAGEARLMIAGGVESMSRAPFVMPKAESAFSRSNAVYDTTIGWRFINKLMKQHYGV
                     DSMPETAENVADDFQIEREAQDRMALASQQKAAAAIAAGHLAQEIVPVHIPQKKGDPI
                     IVSQDEHPRATTMEALAKLKGVVRPDGTVTAGNASGVNDGACALLLANEEAAKAYGLT
                     PRARVVGMAVAGVAPRIMGFGPAPAVRKVLELTGLSLAQMDVIELNEAFAAQGLAVLR
                     DLDLADDDRRVNQWGGAIALGHPLGASGARLATTAVSQLHTLGGKYALCTMCIGVGQG
                     IAVILERV"
     gene            complement(16958..17602)
                     /locus_tag="PK518_03275"
     CDS             complement(16958..17602)
                     /locus_tag="PK518_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRM47960.1"
                     /translation="MSNYQKRSKTELAQRVAQDIFDGAYVNLGIGQPTLVANHLPAGR
                     EVVLHSENGILGMGPAPAAGQEDYDLTNAGKQPVTLLPGGSFFHHADSFAMMRGGHLD
                     ICVLGAFQVSSTGDLANWSTGEPGAIPAVGGAMDLAVGAKATWVMMDLLTKTGECKVV
                     EECSYPLTGLACVKRIYTDLCTLECTPDGLRLIDTVPGLTHAELESIVGLPIRQ"
     gene            complement(17630..18325)
                     /locus_tag="PK518_03280"
     CDS             complement(17630..18325)
                     /locus_tag="PK518_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HRM47961.1"
                     /translation="MINKIADSVAQALSGVKDGATVLIGGFGTSGNPIELIDGLIAHG
                     ARELTVVNNNAGNGDAGLAALLKSGQVRKIICSFPRQADSWVFDELYRAGKIELELVP
                     QGNLAERMRAAGAGIGAFFCPTAYGTELAKGKETREINGKHYVLEYPIYGDVALVKAE
                     KGDRWGNLTYRMSARNFGPVCAMAAQFTVATVHEIVELGELDPEAVVTPGIFVSQVVK
                     VPRVATKAGGIRE"
     gene            18428..19231
                     /locus_tag="PK518_03285"
     CDS             18428..19231
                     /locus_tag="PK518_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRM47962.1"
                     /translation="MLDTPLSVSTTHKPGDSYVQSFARGLEVIRSFNASAPQQTLSEV
                     AARTGLTRAGARRILLTLQTLGYLESDGRLFRLTPRILDLGFAYLSSTPIWDLAEPMM
                     EALTERVHESCSAAVLDGLDIVYVLRIHTHKIMSTNLAVGSRLPAFWTSMGRVLLAAL
                     PEEDLHARLAALPRQAFTRHTALDDQVLLARITQARQQGWCLVDQELEEGLISVAAPL
                     KNRAGQVVAALNISGQANRTSTEMLREQLLPELLATAQAISRTVGLRRA"
     gene            19258..19692
                     /locus_tag="PK518_03290"
     CDS             19258..19692
                     /locus_tag="PK518_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HRM47963.1"
                     /translation="MFNPTQADVRRFFCAAHAKQQAGGPMEAIETLAGLWIAEHPEYH
                     ADLADADAAVARNYDETPTRTNPFLHLSMHLSISEQCSIDQPRGIRQAVELLSRRLGS
                     LHDAHHAAMECLGSMLSDAQCAGRAPDGQAYIACVQRRATRD"
     gene            complement(19653..20549)
                     /locus_tag="PK518_03295"
     CDS             complement(19653..20549)
                     /locus_tag="PK518_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM47964.1"
                     /translation="MRLQTLVALTDFSPSAEQAVARAALLAAEHKAELHLLHGSAGPD
                     VKHVDPQARLEQLARQLARRHGIVVKALAHGGAGLVQRILHAAAGADLLVLGHSLAAG
                     VAAMWRGDMLSQVLRASPCPVLVVQRATRGAYRHVLVKMDAWPQSQALVHFAGALDEA
                     AAVELFHRARPGDRSGPCVAELAHGLRDELRRRMHGRRVRLSDVFDARRNRVGLKLGQ
                     GVDDKARQLAVQQHRSGADLVALTQQRRRWLGDWLQGGMLRHLLGHADCDLLVYPHDR
                     PQATPGGRALQSRVARRWTQAI"
     gene            complement(21002..21661)
                     /locus_tag="PK518_03300"
     CDS             complement(21002..21661)
                     /locus_tag="PK518_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM47965.1"
                     /translation="MKKLLTTVFGLSVACATGLLHAEEPKGDAKAGAGKVAMCIGCHG
                     ISGYQANFPEVYKVPKIGGQDAKYITAALEAYKKGERKFPSMRAIADSLTEQDMADVA
                     AYYSGHGRATELQAQPAREPNAQVAQLLQKGACVSCHGENFAKPIDPSYPQIAGQYAD
                     YLYVALRQYKDGSGAYFGRSNAIMAGIAKQFTNAELKALAGYVGSLDTNLQFVPEARF
                     R"
     gene            21769..22611
                     /locus_tag="PK518_03305"
     CDS             21769..22611
                     /locus_tag="PK518_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM47966.1"
                     /translation="MKFQGSENYVATQDLMLAVNAAIKLQRPLLVKGEPGTGKTMLAE
                     EVAQALNMPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDERVKDIHNYIVKGVLWQ
                     AFTADAPVALLIDEIDKADIEFPNDLLRELDRMEFYCYETREMVRAKHRPLVFITSNN
                     EKELPDAFLRRCFFHYIKFPEAETMKKIVDVHFPTLKSELLTAAMKTFYDVRGLPGLK
                     KKPSTSELIDWLKLLVAEEIPLEALQSADNKVSVPPLVGALLKNEQDVSLFEKLVFMN
                     QRNR"
     gene            22625..22864
                     /locus_tag="PK518_03310"
     CDS             22625..22864
                     /locus_tag="PK518_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47967.1"
                     /translation="MSATQRLLLEGLSDAVGFVGGALAGYGLGLLLGMDIFDQGYGSA
                     SILGIALVGLGGGLGLHLARRWRAARMTARQGAQP"
     gene            22861..23463
                     /locus_tag="PK518_03315"
     CDS             22861..23463
                     /locus_tag="PK518_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM47968.1"
                     /translation="MSFVEPVTLRRGGLLLEPLALSHEEGLRQAAADGELWRIRVTSV
                     PEPQDTRTYIEAALQGRAEGQRFAFAVIDEASGRVLGSTSYHDILPAVRRVEIGYTWY
                     AKSVQRSHVNTTAKLLMMGHAFEQLQCHVVGWRTDNFNFASQRAIERLGARKDGVIRG
                     HALRRDGTIRDTVMYSMRAGEWPEAKAQLLYLLERHARTE"
     gene            23482..24669
                     /locus_tag="PK518_03320"
     CDS             23482..24669
                     /locus_tag="PK518_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRM47969.1"
                     /translation="MLIDFFYTLRAAKLPVSVKEFMGLLEALQVGVVGPHSDDAWSLD
                     DFYYLARTALVKDEKHFDKFDRAFAAYFKGVEMLADFTKEIPADWLRKMLERELTPEQ
                     KAAIEKMGWDELMETLKKRLEEQKGRHEGGNKWIGTGGTSPFGHGGYNPQGIRIGGAG
                     KNKSAVKVWEQRAYRDYDDQQELGTRNIKVALRRLRKFAREGHELELDLPDTIRSTAA
                     NAGYLDIKMVPERHNNVKVLLLMDVGGTMDEHIQRVEELFSAVKSEFKHLEFYYFHNC
                     VYDFMWKNNRRRFAEKFPTWDIIRKYNKDYKLIFVGDATMSPYEILQPGGSVEYNNEE
                     PGAEWLQRLTHAFPKFAWINPEPQGVWQYRQSISIIQQLMGQRMYPLSLKGLEDTMRL
                     LSK"
     gene            24675..26540
                     /locus_tag="PK518_03325"
     CDS             24675..26540
                     /locus_tag="PK518_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRM47970.1"
                     /translation="MFHMRTTPARWPAVLFLTLALAVLPACGTLQGAPGAEPSEPQAE
                     RDAFTLDIQAEDAAIRDYLGRHLELQRFRQLPDLQSQELARLLQVADANARELLATLG
                     YFSPEITLQLQDKPTPAKDEPAQTVRLAVQPGPRTQVESVDIAIAHADTPDLKRRQAQ
                     LQRNWGLPEGEPFTQQAWDEAKAQGLRSLQARRYPQARLVDSRADIAADSAQARLSVQ
                     YDSGPAYRFGPLQIRGGERYDSVGLARIALLPTGQDYREGQLLDAQQRLAASGYYDAV
                     FLTLDSDAPDPQAAPVIAQVRDAPLQKLVFGVGISTDSGPRLSMDHSHNRLPALSWRA
                     VSKLALDNKRRLVSSDWTALPSASGWRWFSGAQLQRESTGSFEVNSTRLRAGRTQNTD
                     HIDRSYYLQHDTADSQGIGAPASSSAISANFGWTGRYFNNNNAPTRGWGLAGEIGLGS
                     TLRPTREPFMRTQLRWQSFIDLGRVDLGNKVRRASRLSLRVEGGAVLARADADIPVTQ
                     LFLTGGDTTVRGYGYRSIGAGINNGQTYGGRYMTVASAEWQRPIVLRGNRTDFESAVF
                     IDAGAVADKPGELSARVGLGAGLRWRSPVGPLQADLGYGLQSHELRLHLRLGYTF"
     gene            26543..>27534
                     /locus_tag="PK518_03330"
     CDS             26543..>27534
                     /locus_tag="PK518_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF490 domain-containing protein"
                     /protein_id="HRM47971.1"
                     /translation="MSPGHHPNPFAAAAAPRPQPTPPRRSHRVLLALELLLATALALL
                     LALWWWAGSSGSLASTLSRATHYLPEGQSLETREVTGSLRAGGRIGWLRWSSPTLTVE
                     VQDARIAWQLAPLLRRQLQFSELQATQITLTPQGEASSEPPEPLQQLLLPLQVELPFA
                     FERITWAGASPVTIEGLAGNYRYDGTQHRLEVQQLNLAQGRYALQAQLQARAPMALTA
                     QLDGSVHTALPGSTTQLQAQARAQLQGSLASAAGRLNVTAQLRGTGTEARPPLHADLQ
                     ASLAPWAGQPLLQATAELQALDLAALWPQAPRTRLTGSAGLEPADNGWRLHAAL"
BASE COUNT         4827 a         9383 c         8923 g         4401 t
ORIGIN      
        1 aacacgatga gctgcaccac gcgcggcttg gtcagcgcgt agaactgcgc caggcgcgac
       61 ggcagcgccc cggcaccggc catgccgaat gggccggcgg gcgccggcgc gggggatggg
      121 gtgggcgcca ggctcatgca gaaaccctct ttgcggccgg ctgccttgcc tgcacatggg
      181 ctgccctggc ctcggccgtg gcgctgcggc tggcaaccag cgcccaggtc agcaccacca
      241 ccagcgccgc cgccccgccc gtatgcagca ccgccgccac cagcggccag tccaggatca
      301 cgttggacag gccggtgagc aactgcagcg ctcccaggcc cgccagccag cgcgcctggt
      361 cgcgcagcaa ttgcgcgcgc agcagacgcc acaccaggag cgccagcacc agcagcacgc
      421 cataggccgc caggcggtgc acgtagtgga tggcggtcag ggcggcaaag ccgatcaggc
      481 tgccgtcctg cagatggccc agttcgcgcc agatctggaa gccctcgctg aaggccatct
      541 cgggccacca gctgccctgg cactgcggaa aggtggtgca ggccagcacc gcatagttgg
      601 tactgaccca gccgccaagc acgctttgca gcgccaccag cgccatcgcg ccagccagca
      661 accagcgcag gccgcctggc agcgccacgg gggccacgcc cttggtgtgc tgcgtctggc
      721 gcacggcctg caggcacagc agggccagca gcaccaggcc gccaatcagg tgcagcgtga
      781 cgatggccgg aaacagcttc atggtcacgg tgagcgcgcc aaacgccccc tgcagacaga
      841 cccagaccag cgtcgccgtg ggccaccagg gactgagcac gcggttggcg ccgcgctggc
      901 gccaggcgcc cacggtgagc gcaatgatga gcacgcccac gccggtggcc aggtagcggt
      961 ggatcatctc cacccaggcc ttgccatgcg tgaccgggcc ggtgggcatg gcctgctgtg
     1021 cggcggagat ctcggtatgc gcacccaccg ggctggcgct gccgtagcag cccggccagt
     1081 cggggcagcc caggcccgag tccgtcaggc gcgtgaaggc accgaacatc accaggtcaa
     1141 aggtcaggaa cagcgtcaga accgtgagcg cctgcaggcg ccgcaccggc gtgctgccgc
     1201 ggctcctcca ccacacccaa gccagggggc cgagggcgat caccaggccc agggccatga
     1261 cctcaagcat gggcgccaaa tcgtagagcg cttgtgtgtc gttcatggtt gctccttggc
     1321 acggcccggc tgatcccacg acgcggaggc gcgcagcagg cgctccaggt cacgcctggc
     1381 ctgggtcgcg ccggcgccgt ccatgcgcgc cggaaagcgc atcatccact gaccctgcgg
     1441 atcgaccaca tacaggtgct cggacagccc atggcccgcc tgcggcgcca gccactgcgc
     1501 cagcatggcg tcatcggcgc gcagtaccgt cgcctgctcc agcgccgggg cgatgtccgc
     1561 gggcagcgcg gcatcgtcgc tgaccagcca gacccagtcc aggcgctcct tctcgcggcc
     1621 catggcctcg cgcatctgac gctgcaggta caggttgttg cggcagtctg cgtcgcaagc
     1681 ggcagcgccc acgctgacca gcaaccactg gtcgcgcagt tcgcccagtt tgcgcggctg
     1741 gccatcgagc gccaccacca gggcctcggc cggcatgggg cgctggggtt cgaccagctc
     1801 gccaaagttg cgccgtcctt ccgggcggat cacgtagtag gtgaaatacg aggcaatcac
     1861 cggcgcggcg cacaccagca acacacccag caactgccag cgccctatgc ggcgcgcgcg
     1921 ctgcggccgc tgccccgtgg gctcaggcgt ctgacttgcc gccaagggag ggtaaacgtc
     1981 ggacaaattg gaaccagaca taaagaatga acaccaggcc ggaaagcccg aaccattgaa
     2041 acgcgtagcc gtaatgcttg tcgacaccgc tgtcggcagg cgcccagtcg cgctgcagcc
     2101 catcactggc gccgccagtc tgcaccacca ccaggggcag cagcggcagg ccggtctccg
     2161 cgccataggc agccaaggcc agattttgcc ggatgcgcga agccccctcg ccctcgccgg
     2221 ccggagcaaa ctcatacagc cgcgcaactg cggcggccac atgaccatga atctgcacat
     2281 tgccggcagg tgtgacaacg tcgggcagac gggtacgctc ctgaaaatcg cgcggtgccc
     2341 agccgcgctg caccagtacc acgccttgtt cgcccggcag gcgcagcggc gtcagcaccc
     2401 agaaacccgg ccgcccctgc atctggcggt tgtccagaaa caccgtggcc tgaggcaccc
     2461 actgcccttg cagttgcacg ttgcgctgca gcaaggcagc cacgccaccg acccgggccc
     2521 gctccaggtc gcggcccacc aataccggca tttgctcgcg cgcgaccagc atggcctgca
     2581 aggcctgctt ctgcgcggca cgcgataact gccaggcccc gagcgtggcc gtcaccgcca
     2641 tcgccaccag cgcagccagc gtgagcagcc agaagcgcca accgaaacga gcctgtgcgg
     2701 gactgataat gggatccatg aaaattttgg tggccttggc gtttctcgcc atcatcgcaa
     2761 gcctgggatc ggcgctgttt ttcatgatgc gcggagatgg caacgaccgc cagcgcagca
     2821 aacacatggc gcgcgcgctg gcgctgcgcg tgggcctgtc gattgtgctg tttttatgcc
     2881 tgctgctggc gtggaagctg ggctacatcc agcccacggg tttgccagcg gtcatgcgtt
     2941 gaagcaaaaa agcgccctga ggcgctttct tgcggcgctt gcatggctca catccagtac
     3001 accaatatgt acagcccgag ccagaccacg tccacgaaat gccagtacca ggcggcaccc
     3061 tcgaaaccga aatgccgctc ggccgtgaaa tgtccgtttt gcaggcgcag cgtgatgaag
     3121 aacagcatca acatgcccac gaacacgtga aagccatgaa agccggtgag catgaagaag
     3181 gtggagccgt aaatgcccga gctcagcttg aggttgaggt cggtgtagag gtgaaagtac
     3241 tcatacgcct gcacacacag gaaggtaacg ccgagcagca cggtgatcca catgaagcct
     3301 atggccttgc tgcgctgatc ctcgcgcaag gcgtggtggg cgatggtcag ggtcacaccc
     3361 gagctcagca gcagggccgt attgatggtg ggcagccaga acggccccac ggtctggaaa
     3421 ggatccacca cgttggccgg cgaggccgtg gcgccggcca ccacgctggg ccaggcggcc
     3481 ttgaattctg gccacagcaa tgcattttcc aggctgccca gtgccggcac cgagtgcgca
     3541 cgggcccacc agagcgccgt gaagaaggcg ccgaagaaca tcacctcgga gaagatgaac
     3601 cagctcatgc tccagcgaaa ggacacatcg accttgcgcc cgtacaggcc ggactcgctt
     3661 tcgtgcgccg cctcgcgaaa ccaccggaac aacacgacca gccaccagag cagccccgcc
     3721 gcgaacgaat acttgcccca gtcgtggccg ttgatccaca tggcggcgcc caggatgacg
     3781 aagaacagcc ccaccgcgac cagcaccgga tggctggact gcgcgggaac aaaatagtac
     3841 ggcggcttgg cgccctggat cgatgcactc atgtcagctc ctctgtctcc ggtttatcca
     3901 acttctgtcg ttgcggatgg cgcggtcaaa ccagccaatt gaccaatgcc atgagcagca
     3961 gcaccagcag aaccagggcc agcagcccca tgcccaatac ctgcagtggc cgcagcgatt
     4021 cctgatcctg gcgatgccga tcggcattgc gcaccccgaa caaggcccag gcaatggtgc
     4081 gcatggtgcg ccacagagat gcgctggggc gcgccggcgg acgatctcct gcttccgtca
     4141 tgacgcccca gcccctggtg tctgcactgc cccggccgtc gattgcggcg ccgacggaat
     4201 cttgccgccc acttcaaaga aggtgtagga cagcgtgatc gtcgtcacat ccttgggcag
     4261 gcgcgggtcg atcacaaaca ccaccggcca ttccttcttc tcgccggggt cgagcgtgta
     4321 ctggctgaag caaaagcatt cgagcttgtt gaaatgcggc gcggcctggc gtggcgcgta
     4381 gctgggaatg gcctgagccg acatgcgcct gtcctgcacg ttctcgaact cgtagagcac
     4441 ggtcgccatc tcgccggggt gcacctgcat gctgcgcttg gccggcttga actgccaggg
     4501 gccgcgggca ttggcatcga actcgacggt gatggtgcgg gtggtgtcca cctgagtgtt
     4561 cttcaccagc gcacgtgcct cgtccgcggc gacaccattg ccggggacct ggcgctcggt
     4621 gagcgagagg atgttcacgc ccgtggcctc gcagatcgcc ttgtaggcgg gaatcagcag
     4681 atagccaaag gcgaacatgc ccagggtcac gaccagcagc ttgccgagca tcctgaggtt
     4741 gtcgctgcgc cgtgccatgc ctgcctccgc cgtcaatggc ccagcacgaa ataccgctgg
     4801 acaatgaagc cgacaaacaa tgccagcgcc accgcagcca gcatcagccc gagccgggca
     4861 ttgcgcttgc gttgctcttg tgacgtcatc tcgctactgc tcctgtcggc ccgcgctcag
     4921 ccaatcaccc gggtcgccgt ggcgtccagc ttgggcggct cctcaaaggt atggaacggc
     4981 gccggcgaag gcacttccca ctccaggccc tcggcgccct cccagggccg ctgcggcgcc
     5041 ttgtcgccct tgccgcgcag catgggcagc accacggcga agaaaaaata cacctgtgcg
     5101 gcaccgaaga agaagccgcc gatggaggca atcatgttga agtcggcaaa ctgcatcggg
     5161 tagtcggcat agcgtcgcgg catgcctgcc agccccagaa agtgcatggg aaagaaggtg
     5221 acgttgaaag agatcatcga ccaccagaaa tggatcttgc cgcgcgtctc gttgtacatc
     5281 acgccgcacc atttgggcga ccagtagtag tacgcgccat agatggcgaa cagcgaaccg
     5341 gccaccagca catagtggaa atgggcaatg atgtagtagg tgtcctggat ctgcgtgtcc
     5401 accggcacca cggccggaat cacgccggtg aagcccccga tggtgaacac gaagatgaag
     5461 cccacggccc acagcatggg cgtttcaaac gtcatcgagc cacgccacat ggtcgccgtc
     5521 cagttgaaca ccttcacgcc cgtggggatg gcgatcagca tggtgccgta catgaaaaac
     5581 agctgtccgg tgaccggcat gccggtggtg aacatgtggt gcgcccagac gatcatcgac
     5641 agcgtggcga tggcggcaat ggcgtacacc atggaggtgt agccaaacaa cttcttgcgc
     5701 gcgaaggtgg gcacgatctg gctgatcatg ccgaaggccg gcaggatcat gatgtagacc
     5761 tcggggtggc caaagaacca gaagatatgc tggtacatca ccgggtcgcc gccgccggcc
     5821 gggttgaaga aactggtgcc gaagtggcgg tcggtcagcg tcatggtgat ggcgccggca
     5881 aacaccggca tcacggcgat cagcaggtag gcggtgatca gccaggccca gcagaagatg
     5941 ggcatcttca tcagcgtcat gcccggcgcg cgcatgttca ggatggtgac gatgatgttg
     6001 atggcaccca tgatggacga ggcgcccatg atgtgcagcg caaaaatggc cgcgtccagc
     6061 gacatgggca tttgcgccga cagtggcgca tacagcgtcc agccgccggc gggtgcgcca
     6121 ccggccatga agaacgatgc cgccagcatg atgcccgctg gtaccagcag ccagaagctg
     6181 aggttgttca tgcgcgcaaa cgccatgtcc gaggcgccga tctgcagcgg gatcatccag
     6241 ttcgcaaagc ccacgaaggc cggcatgatg gcgccgaaca ccatgatcag accgtgcatg
     6301 gtggtgaact ggttgaacat ctccgggttc acgatctgca ggccgggctg gaacagctcg
     6361 gcgcggatca gcatcgccag cacgccgccg atcatcagca tggtgaacga gaacagcaga
     6421 tacagcgtgc ctatgtcctt gtggttggtg gcgtacaccc agcgccgcca gcccgtgggc
     6481 tggtggtgat ggtggtcgtc atggccggcg gcatgcccat ggttgtccaa aactgcgctc
     6541 atggtcgaat cctcaatacg ttgcgttggg ccttgggttt cacttggcgc gcagggccac
     6601 gatctcggcg ggctgcacca gctggccggt cttgttcgac caggcgttct tggtataggt
     6661 gaccacggcc gccaggtcgg tatcgctcaa ggcggcccag gagggcatgg caccattgcc
     6721 ggcgcccttg agcacaacct gaatctgcgg cgcatggttg gcgttcttga cgatggccga
     6781 gccatcgagc gccttgatgg gcccggcccc cttgccgttg gcctggtggc aggccgcgca
     6841 gttggcggca tagaccttgg cgccccgcgc ctgcagatct gccagcgccc agaccttgtt
     6901 cggatcatcg cgcctggcgg cggccttgtt ttgctgatcc gccacccagg ccgtgtactc
     6961 cacctgcggc agcaccttca cgtggatcgg catgtaggca tgctccttgc cgcacagctc
     7021 cacgcactgg ccgtaatagt cgcccacctg ctcggcgcga aaccaggtgt cgcgcacaaa
     7081 accggggatg gcatcctgct tgaccccaaa ggagggcacc atccacgaat ggatcacgtc
     7141 gttggcggtg gtgatgatgc gcaccttctt gttcaccggg accaccagcg ggttgtccac
     7201 cttgagcaga tagttgtcgc cctggggtgt gccggcgctg gacagggcgc gctgcgatga
     7261 atccagggtg gagagaaagc ctatgccctc gccctcgcca gtcaggtagt cgtaacccca
     7321 tttccattgg tagccggtga ccttgatcgt cagctcggcg ttggtcgtgt ccttctgcgc
     7381 cacgatgacc ttggtggcgg gcgcggccat ggcgatcacg atcaggaagg gaatcaccgt
     7441 ccaggtgatt tcgacagcgg tggattcatg gaaattcgcc gccttggcac cacgtgattt
     7501 gcggtggtgc cagatcgaat agaacatgac gccgaagacg ccgatgaaga tgaccgtgca
     7561 gatgatcatc agcatccagt gcagctcatg ctgcgcctcg gcgatctttg tcacgggtgg
     7621 gtgcaggttc agctggttta ccgccgggcc gccgggcagg tcttgcacgg catgggccag
     7681 cgtgccccag gaggcgagcg ctaccagcaa cggggaggcc agcttgttgg aaatgcttgt
     7741 catggttctc tcttgtcttt ctagctccgg ttgaccaatc ccctgcggcg tgcactgttg
     7801 ccagacgctg cgcaaagcct tgcgaccacc tgccacccct gccgcatgga acctgcattc
     7861 gccagcgacg gggcagtgca cgactgccgc attttttcaa tcagtccaga ccactttcca
     7921 ggcagcctgt tgtctccacg atccagtgca cggactctct cgttcgcttt cagcatgccc
     7981 agccagcaaa gcacccgcaa tacgcgttag ccccagggaa gcagcgtgaa gaagtgacgc
     8041 acaagaaagg ttctgacaac gcatggtgtc ctcagaactt caaaagatgc gacaagatgc
     8101 acctggcaac acccaggctt caccacgtca ggagagttcc gactctgcaa tcacaattca
     8161 ttctagcgcg cattgatgga ggtcattgac ggcgcaaata gcgccgcaga gcactcagcg
     8221 cgtggatgaa cggcccgcgt gcagcatggc gcgcatgtcc tgcagcgaca cggcgctgct
     8281 gccctccaag cgcacacgcg gcgcgggttt gaaggcgtgg ccgtagatga tctcaaaggt
     8341 cagggccagc tgcccgtccg caccagcgtc ggccaggcgc tgcgccaggg cctgctccag
     8401 ccgcgcacgc cagttgcgcc cacgcaacgc cgcaaagcgc tgcgggtgca ggttgcgccc
     8461 caggccgcgc agctcctgca gcaggcgctg gggcgtggca aaggtcaagg tgatgcgctc
     8521 catgtccatg atgggctcgg caaagccggc atgcaccagc atgtcgcccc agtcgtgcat
     8581 gtcggtcagc gcatggccgg gcgcgggcca gtccagctcg gcatacaggg cgcgcagctc
     8641 gcgcagcgtg tccggcccca ggcaggagaa catcaggtag ccgtccaccg ccagcgcccg
     8701 gtgccattgc gtcaccaggg cctgcggatc cgcggtcatg tgcagcgcca tgttggacca
     8761 caacatctgc acgctggcgg cttcgggctg gccaaagcgc ggcgcgttgg cgatccagcg
     8821 cgccgggctc caccagcgcc tggcaaggcg ttcgcgcgcc agcgcctcac cctggggcga
     8881 agaggtttcg tggatctggc acgcggcctg tgcaaagcgc tcgctcacca gcgcatggcc
     8941 ctgcatgcca ccgcgcaacg gatcccagtc gcaccaggcg gtgggcgcat tcaccatcca
     9001 ctgcagcctg tcctgcatgc gccgcgccac ctcctcgtgc agccagggcg attcgtcaga
     9061 gaccatggca tgccagcgcg cggcggcaac cgggtctatg gtgggcggac gttgttctga
     9121 catggcggga cgcaaatggc ggcgagtata ttgagcccat gcttgacacg cgccccgcag
     9181 ggatatggcg ccggggcctg gcgaccctgg cggccctcgg cgcccggctg cccagccaat
     9241 gcgccgtctg ccatgcctgg ccggcgcaac gcctgtgcgc ggcctgctgc gcgcgctttg
     9301 cgccgcggct ggcgcgctgc gccacctgcg cctgcgtggt gcatggcggc gtggcgcaat
     9361 gcgggcagtg cctgctgcac ccgccgccgc tggacgcctg cctggccgcc gtggactacg
     9421 gctacccgtg gtcacagttg ctggcgcaat tcaaattcca ggacgatccg ggctgggcca
     9481 cggcactcgc cgcgctgatg cgccagacgc cgggtgcgca agagctgctg gagcaggccg
     9541 attgggtgct gcccgtgccg ctggcgcccg ggcggctgcg tcatcgcggc tacaaccagg
     9601 cgctgctgct ggcacgccgg ctggccggag cgcatgtgcg gcccgacctg ctgctgcgca
     9661 cgcgcgaggc cgaggcacaa gcgcagctca cgcgcgcgca gcgcctgcgc aacctgcgcg
     9721 gtgcctttgt gctcgagccg ctgcgcgcgc agcagctggc cggtcgccgc gtgctgctgg
     9781 tggacgacgt gatgaccacc ggcgccacgc tgcacgccgc cgccgcgccc ctgcgcgagg
     9841 ctggcgtggc gcatgtcggc gccctggtgc tggcgcgcac gccgtgacca ccgcagcgcc
     9901 cgcgggacag cggctgacgg tgacaatagc gccatgtttc acatcgtcct cgtcgagccc
     9961 gaaatcccgc ccaacacggg caacgtgatc cgcctggcgg ccaataccgg ctgcacgctg
    10021 cacctggtgg agccgctggg gttttcgatg gaagaccggc tcatgcgccg cgccggcctg
    10081 gactatcacg aatacgccaa gctgcgcctg cacaagggct ggacggcgct gctgcgcgag
    10141 atgcagcccg acccggcgcg catgttcgcc ctgaccaccc atggcacgcg cctggtgcac
    10201 gacacgggct ttctgcccgg cgactggttc ttgtttggcg ccgaatcgcg cggcctgccg
    10261 cccgagttgc gcgagagctt tgcgcccgcg caacgcctgc gcctgcccat gctggccggg
    10321 cagcgcagcc tgaatctgtc caacgccgtg gcggtgacgg tgttcgaggc ctggcggcag
    10381 aacagctttc tagcgccgca ggcttaagaa atcaaacagc gtccagccag tcgcaaatgc
    10441 cctgccactg ctccacatcg cgcgccacgc gcgtgccggc cacgtcgaac accgactgcc
    10501 cctgcgccgc cagctgcaca tagttttgcg tgttacgcag atagcccagc accggcaggc
    10561 ccaggctgtc cacaaagttg cgcagcttct cggcgacgat ggtgcgcgga tcgacgcgca
    10621 tgccgacgat ggccacgcgc tccagcccgc cggggcgctg ggccgccagc tcgtccaggt
    10681 actggccggt ggcgtagatg tcgaacaggc tgggctgcag cggcacgatg atgcggtcgg
    10741 ccagcttcag cgcgtccttc aggcgccagc cgcccaggcc ggccggcgtg tccagcacgg
    10801 catgggtggc ctgcttcggc ggagcgctga tccagtcggc atccacctcc caggccccaa
    10861 tgggccgagc cgtggccggg cgctgctgca gccacaggcg cgcggactgc tggcgatcgg
    10921 catcgcccag caccaccgta tggccgcggc tggcaaagta acccgcgatg ttggtggaca
    10981 gcgtggactt gcccacgcct cccttgggac tggctacagc aatgactggc atctgaaatc
    11041 cctctctggt ttgaataaaa caagcctcta gcgcccatcc ataaaacgca tatagctatt
    11101 aatttagaag cattactggt gctgcgtacc acgcttgacg aagaacagcc cggcgcccac
    11161 cagcatcaac aggccgccaa acacgcggtt ttgcgcacgc atggcgctgg cgttctgcat
    11221 ccagcggcgc agcacgctgg cgctggcggc atagccgttc atcacgatca gatccaccga
    11281 caccagggtc agggccatca ctagcagctg gctccagagc gggcgcgctt cactcatgaa
    11341 ctgcggcagc accgccacca tgaacaaaat gcccttgggg ttggtggcgt tcgtcagcgc
    11401 accggtgagc acacgccggc gccagctcgc cggcgcggcc agcaggtcgc cgcccagcgt
    11461 gccggccgag cccgcgcgcc actggctgaa acccaggtag atgaggtagc aggcgcccag
    11521 caccttgacc acggtgaagg ccagctcgga ggcaatcaac agcgaaccca ccccggcgcc
    11581 cgcgacgatg aggatgaaca gcaggcccag ctccagcccc aggatggtgg cgcccgtctt
    11641 gcgcacgccg taagacagac catggctcat ggacagcacg gcacccgagc ccggcgaaat
    11701 ggcgatcacc cacgacgccg caaaaaaagc catccaggtg tgccagtcca tatcacgtcg
    11761 tgctgggaaa agtgttgcaa gggagaacgc attttatcgg ccgcgcagct gctggccacg
    11821 ggcctggctt tgatgctgca tgatggtgcc cggttgccgt tgaccaccga tacgcggcgc
    11881 gcccgagacg ccgttgcccc cctctcattc gcgaggtatc ccatgaaccg ccgaatcatt
    11941 gtgctctcgc tgctgctgtc acccctggct catgcccagc cggaacgcct ggttccctca
    12001 agcccggtgg atagcgtagt gctgtacgtc attcccaccg atggaattcc ggagcaggtt
    12061 gcggccaccg tcgcccggac attgacggaa gacaccggca tgtgggtcaa gtccgcgctc
    12121 caggtgcctt cgggtgtgac cgagccattc gctggcacga accagtaccc cgccgaagac
    12181 tacctgccac ttggcgccac cttcgcgaag cggctgccag acgcggggcc aagaacgtat
    12241 ttcatcgtgc tcacggacaa ggacatcaac tcacgctccc gcaacttccg gttccagtac
    12301 tcgatgcaca gtcccatggc gcgcacctcg gtgctgtccg tcgccaggct gctcttcacc
    12361 agggacggca atccggcctc gggggatgtc atcaggctgc gcatacagaa aatgctgatg
    12421 cgcatcgttg gcgaaatgaa gtttggctgg cagcggacga cggatcccgc cgacctcatg
    12481 tactcaccga tcatgtccat cgacgacatc gaccgtatga gcctcaccca cacggcacaa
    12541 acgcgtggca tcccccgcta tgatttccaa ccttccgcaa ccgagccccc tcggcccctg
    12601 ccaaaccaca tctgaagcat agaggccaac agccgccact tgccactctg gccgcgcgcc
    12661 agcctgcgca atcaagggca ccccggactg tcagaattcc gcccttggct ttcagttttc
    12721 gcttccccca ccatggccca agcccccgtc tggctcacct acggattcac ctacctggcc
    12781 gcggccgtga ttgccgtgcc catcgcccgc gcgctcggcc tgggcgccat catcggctac
    12841 ctggcggcgg gcattgccat agggccctgg ggcctggccc tggtgagcaa tgtgcaggac
    12901 atactgcact ttgccgagtt cggtgtggtg ctgatgttgt ttctggtcgg cctggagctg
    12961 cagcccagcc ggctgtggag cctgcgccgg cccatcctcg gcctgggcat ggcgcagatg
    13021 gccggctgcg cgctactgct gtggggctgc gcctgggccc tgggcctgcc ctggcgcgtg
    13081 gcactggtgg gggcgctggg gctggcgctg tcatctacgg cgatcgcgct gcaggtgctg
    13141 aacgagcgca acctgctgcg caccgacagc gggcagaagg cgttctccat cctgctgttc
    13201 caggacgtgg cggcgatccc catcctggcc ctgctgccgc tattgggcgt ggcggcgcgg
    13261 gccgaggcgc tgcagccgca tgcgccgctg gacctgctgc ccgaggcgct gaagatcatc
    13321 gccgtggtgg ccgccatcgt gtttggcggg cgctgggtgc tgcggccgct cttgcgctgg
    13381 attgcaaaaa gcaagacgcc cgagatcttc accgccgcct cgctgttcct ggtggtgggc
    13441 attgccatgc tgatgctgac cgtgggcctg tcgatggccc tgggcgcctt tctggccggc
    13501 gtgctgctgg ccgacagtga gtaccggcgc gagctggaga ccgacatcga gcccttcaag
    13561 ggcctgctgc tgggcctgtt cttcattgcc gtgggcatga gcatcgactt tggcgtgatc
    13621 gcgcaccacc ccttcgccat gctgggcctg ctgctgggct ttctggccat caaggcggcg
    13681 gtgatctggc tgctggcacg cgtgaccgcc atggcctacc aggagcgccc gctgttcacg
    13741 ctgctgctgg cccagggcgg cgagttcgcc ttcgtggtgt tccagtccgg cgccacgttc
    13801 ggcgccattg cgccggacca cgcctcgctg ctgataggcg cggtggcgct gtccatgctg
    13861 atgggcccgt tgctgctggt gtggcttgac cgcgtgctgc tgcagcgcca cgccacgctc
    13921 caaagcgcgc cgcaggccca ggagatttcc gagccgcaga gcgcgccggt gatcattgcg
    13981 ggcttcgggc gctacggcca gatcgtggcg cgcatgatgc tggcccaggg cgtgccggcc
    14041 acggtgctgg accacagcgt ggagatgctg gaggtggccc acacctttgg ctaccgcgtg
    14101 ttctacggcg acgcgacgcg cgtgaacctg ctgcgcatgg cgggtgcgca gcaggcccgc
    14161 atcctggtgg tggcggtgga tgcgccggag cagtccctca agatcgtgca gctggcgaaa
    14221 aagcatttcc cgcacctgca catcgtggcc cgcgcgcgcg acgtgacgca ttggcacgcg
    14281 ctgcgcgacc tgggcgtgca gcatgtggag cgcgagctgt tcgagtccag cctgcgcacg
    14341 gcgcgcacgg tgctggagct caccggcctg tcgcaggccg acgccgacgc cctggccgag
    14401 cgctttcgcg cccacaacat cgccctgtcc gagcgcatgt acgagcacca caacgaccgc
    14461 gaggccatga tcgccgtcgc caggcagggg cgccagcagc tggtggagca gctggccaag
    14521 gagcgccagg agcgcatggc cgcggccgag gccggcagcg ggctgacgcc ggacaagccg
    14581 cagcccggca aatcttcata agaaaaacgg ctgtagcgcc cattaaacgt gcgctataag
    14641 ctcttatatt tatagtgaca tgatcaatcg accgtggcac ccgaatcctt caccaccttg
    14701 ccccatttgg ccgtctcggc cttgatgaag gcggcgaact gctcgggcgt ctcggccacc
    14761 acgtcgccgc cctgctcggc gatcttcttc ttcacgtcgg gctggttcag caccttgacc
    14821 agggcggcat tgagcctgtc cagcacgggc ttcggagttg ccgcgggggc gaacaggccg
    14881 aaccaggaca tggcctcgta gccgggcacg ccggcctcgg cgatggtggg tatgtcaggc
    14941 agctcgggcg agcgcttggc cgtggtcacg gcgatggggc gcagcttgcc cgagcgcaca
    15001 tgctggatgg ctgacggcat gttgtcgaac atgatggcga tctgcccacc cagcaggtcg
    15061 gtcatggccg gggcgctgcc cttgtagggc acatgcagca ggtcggtgcc ggtcatgctc
    15121 ttgaacagct cgcccgacac atggggcgag gctccgctgc ccggcgagcc gtaggtcacc
    15181 ttgcccgggt tggccttggc gtaggcgatc agctccttga cggtcttgta gggctggctg
    15241 gggtgggcca ccagcagatt cggcaccgtg gccacgcgcg agatgggcgc gaagtccttc
    15301 accgggtcaa agggcatctt cttgtacagc gcctggttga tggcgtgcgt gcccaccgtg
    15361 cccatgaaca gcgtgtagcc gtccgccggc gccttggccg ccagcgaggc gccgatgttg
    15421 ccaccggcgc cggcgcggtt gtccaccacg aagggctggc ccagctcggt gccaaggccc
    15481 tggccgacga tgcgcgccag gatgtcggtg gtgccgccgg gggcgaacgg cacgacgatg
    15541 gtcaccggct tggccgggta gttctgcgcc agcgcgccca ggggcaaggc ggcggcggcc
    15601 aggccggcca gcaggctgcg gcgggtgatg cgtatcgggg tcatcctgtg tcctttttct
    15661 gtcggcaatg gtcgggcgtc aaacgcgttc aaggatcacg gcgatgccct ggcccacgcc
    15721 tatgcacatg gtgcacagcg cgtatttgcc gcccagcgtg tgcagctggc tcacggcggt
    15781 ggtcgccagg cgcgcgcccg atgcacccag cgggtggccc agggcgatgg cgccgcccca
    15841 ctggttgacg cgccggtcgt cgtcggcaag atccaggtcg cgcagcacgg ccaggccctg
    15901 ggctgcaaaa gcctcgttga gctcgatcac gtccatctgc gccaggctca atccggtgag
    15961 ctcgagcacc ttgcgcacgg ccggcgccgg gccaaagccc atgatgcgtg gcgccacgcc
    16021 ggccacggcc atgccgacca cgcgggcgcg cggcgtcagg ccgtaggcct tggcggcctc
    16081 ttcgttggcc agcagcaggg cgcaggcgcc gtcgttcacg ccagatgcgt tgccggccgt
    16141 caccgtgccg tcaggccgca ccacgccctt gagcttggcc agcgcctcca tggtggtggc
    16201 acgcgggtgc tcgtcctggc tgacgatgat ggggtcgccc tttttctgcg ggatatgcac
    16261 gggcacgatc tcctgcgcca ggtggccggc agcaatcgcc gcggcggcct tttgctggct
    16321 ggcgagcgcc atgcggtcct gggcctcgcg ctcaatctgg aagtcgtcgg ccacgttctc
    16381 ggccgtctcg ggcatggaat cgacgccgta gtgctgcttc atcagcttgt tgatgaagcg
    16441 ccagccaatc gtcgtgtcgt acacggcgtt gctgcggctg aaggcgctct cggccttggg
    16501 catgacgaag ggcgcgcggc tcatgctctc cacgccgccg gcgatcatca gccgcgcctc
    16561 gcccgccttg atggcgcgcg ccgccgtgcc cacggcgtcc aggcccgagc cgcacaggcg
    16621 gttgatggtg ccgccaggca ccgcaatcgg caggcccgcc agcagggcgg ccatgcgcgc
    16681 cacgttgcgg ttgtcctcgc cggcctggtt ggcgcagccg tagagcacgt cgttcaccgc
    16741 cttccagtcc acgccggggt tgcgctccat cagcgccttg atgggtatgg cccccaggtc
    16801 gtcggtgcgc acgccggcaa gggcgccgcc gtagcggccg aagggggtgc ggatggcgtc
    16861 gcagataaag gcttgggtca tgttgtctcc tgtgaggtga tttaaaaaag agagctgctt
    16921 gcgcttatcc agcaagcgtt acagccatat ttaatgctta ttgccgtatc ggcaggccga
    16981 cgatgctttc cagctcggca tgcgtcaggc ccggcacggt gtcgatcagc cgcagaccat
    17041 cgggtgtgca ttccagcgtg cataaatcgg tgtagatgcg cttcacgcag gccaggccgg
    17101 tgagggggta gctgcactcc tcaaccacct tgcactcgcc ggtcttggtg agcaaatcca
    17161 tcatcaccca ggtggccttg gcgcccacgg ccaggtccat ggcgccgcct acggcgggga
    17221 tggcgcccgg ctcgcccgtg ctccagttgg ccaggtcgcc cgtgctgctc acctgaaacg
    17281 cgccgagcac gcagatgtcc agatggccgc cgcgcatcat ggcgaagctg tcggcatggt
    17341 ggaagaagct gccgccgggc agcagcgtca cgggctgctt gccggcgttg gtcaggtcgt
    17401 agtcctcctg gcccgctgcc ggggccgggc ccatgcccag aatgccgttc tcggagtgca
    17461 gcaccacctc gcggccggcc ggcaggtggt tcgccaccag ggtgggctgg cctatgccca
    17521 ggttcacgta ggcgccgtcg aaaatgtcct gggccacgcg ctgggcgagt tcggttttgc
    17581 ttcgtttttg atagttgctc acgcttgtat ctcctgcgct ctactggtct tattccctga
    17641 taccaccggc cttggtggcg acgcgaggca ccttgaccac ctggctgacg aagatgccgg
    17701 gcgtgaccac ggcctcgggg tccagctcac ccagctccac gatctcatgc accgtggcca
    17761 cggtgaattg ggcggccatg gcgcacaccg ggccgaagtt gcgcgccgac atgcggtagg
    17821 tcaggttgcc ccagcggtcg cccttctccg ccttgaccag ggccacgtcg ccgtagatcg
    17881 ggtactccag cacatagtgc ttgccgttga tctcgcgcgt ctccttgccc ttggccagct
    17941 cggtgccata ggccgtgggg cagaagaagg cgcctatgcc ggcgccagcg gcgcgcatgc
    18001 gctcggccag gttgccctgg ggcacgagtt ccagctcgat cttgccggcg cggtagagct
    18061 cgtcaaacac ccagctgtcg gcctggcgcg gaaagctgca gatgatcttg cgcacctggc
    18121 cactcttcaa aagcgccgcc agtcccgcat cgccattgcc ggcgttgttg ttgaccaccg
    18181 tgagctcgcg cgcgccgtgg gcgatcaggc cgtctatcag ctcgatcggg ttgcccgagg
    18241 tgccaaagcc gcctatgagt accgtagcgc cgtccttgac gccagagagc gcctgggcaa
    18301 ccgagtccgc aatcttgttg atcatgtctt gccgtggtga agaaaaaaga aaggccggcc
    18361 cgagtccgac acaatatgtt cgaatatcga acttttgttc gtataatgaa ttctaggaca
    18421 aatggccatg cttgacaccc ccctttccgt aagtactacc cacaaacctg gcgacagcta
    18481 tgtgcagtcg tttgcgcgcg gcctggaggt gatccgctcc ttcaacgcca gcgcgccgca
    18541 gcagacgctc agcgaggtgg cggcgcgcac cggcctgaca cgcgccggcg cccggcgcat
    18601 cctgctgact ctgcaaaccc tgggctatct ggagagcgac gggcgcctgt tccgcctcac
    18661 gccgcgcatc ctggatctcg ggtttgccta cctcagctcc acccccatct gggatctggc
    18721 cgaacccatg atggaagcgc tgaccgagcg cgtgcacgag tcctgctcgg ccgccgtgct
    18781 cgacgggctg gacatcgtct atgtgctgcg catacacacg cacaagatca tgagcaccaa
    18841 cctggccgtg ggctcgcgcc tgccggcctt ctggacgtcg atgggccggg tgctgctggc
    18901 ggccctgccc gaggaagacc tgcacgcacg cctggccgcc ctgccgcgcc aggccttcac
    18961 gcgccacacg gcgctggacg accaggtgct gctggcgcgc atcacccagg cgcgccagca
    19021 gggctggtgc ctggtcgacc aggagctgga ggaaggcctg atctccgtcg ccgcgccgct
    19081 caagaaccgc gccggccagg tggtggcggc cctcaacatc agcggccagg caaaccgcac
    19141 cagcaccgag atgctgcgcg agcagctgct gcccgagctg ctggccacgg cgcaggccat
    19201 ctcgcgcacc gtgggcctgc gccgcgcata gcgggccagc gtgctaggct gacgcccatg
    19261 ttcaacccca cccaagccga tgtgcggcgc tttttctgcg ccgcccacgc caagcagcag
    19321 gccggcgggc ccatggaggc catagagacc ctggccggcc tgtggatcgc cgagcacccc
    19381 gagtaccacg ccgacctggc cgacgccgac gccgccgtgg cgcgcaacta cgacgagacg
    19441 cccacgcgca cgaatccgtt tctgcacctg tccatgcacc tgtcgatcag cgagcaatgc
    19501 agcatcgacc agccgcgcgg catacgccag gccgtggagc tgctcagccg ccgcctgggc
    19561 tcgctgcacg acgcccacca tgcggccatg gaatgcttag gaagcatgct gtcagatgcc
    19621 cagtgcgccg gccgcgcgcc cgatggccag gcctatatcg cctgcgtcca gcgccgcgcc
    19681 acgcgcgact gaagcgcccg cccgcccggc gtggcctggg gccggtcatg cgggtagacc
    19741 agcaggtcgc agtccgcgtg tccgagcaaa tgccgcagca tgccgccctg cagccagtcg
    19801 cccagccagc ggcggcgctg ctgtgtcagc gccaccagat ccgcccccga gcggtgctgc
    19861 tgcaccgcca gttgccgcgc cttgtcgtcc acaccctggc ccagcttgag tccgacgcgg
    19921 ttgcgccgcg catcgaacac atcggacagg cgcacgcgtc gcccgtgcat gcgccggcgc
    19981 agctcgtcgc gcaggccatg cgccagctcg gccacgcagg gcccgcttcg atcgcccggg
    20041 cgggcgcggt gaaacagctc cacggccgca gcctcgtcca gcgcgcccgc aaagtgcacc
    20101 agggcctggg actgaggcca ggcatccatc ttcaccagca catggcgata ggcaccgcgc
    20161 gtggcccgtt gcaccaccag caccggacaa gggctggcgc gcagcacctg cgacagcatg
    20221 tcgccgcgcc acatggccgc cacgcccgcc gccaaggagt ggccaagcac cagcaggtcg
    20281 gcaccagctg ccgcgtgcag gatgcgctgc accaggccag caccgccatg ggccagggcc
    20341 ttgaccacga tgccatggcg gcgcgccagc tggcgtgcca gctgctccag ccgcgcctgc
    20401 ggatccacat gcttcacatc aggcccggcg ctgccatgca gcaggtgcag ctcggccttg
    20461 tgctcggcag ccagcagggc cgcgcgcgcg acggcctgct cggcgctggg ggaaaaatcg
    20521 gtgagggcta caagggtctg aaggcgcatg gtgcatgtct cctgtctggt catggagggc
    20581 agcggccaag ccagggccgc cggcggggcg tcatcgactg atagggatgt ggcgccagca
    20641 caaggctgca cggcacaccc atgtggtgag cccggcggtc tgcgagacgc tgatcaggag
    20701 gggagccgcc gggcctgcag atgcggggcc ggcgcggcgg gtttcaacat gacttgcctg
    20761 tgcatggcgc gcgccatcgc ggcaactgcc gaggcatgcc gcgcgattcg cacacaggaa
    20821 gaggaaacca gaaaacgcat gggcgtcatt atagaaaata accactttat cgacaaggat
    20881 ttcactacta tccctgccag attaaagccg gcatttgaca aacccgtaaa tgtcgccatc
    20941 gctggcttgc agatacaaaa aagcccgcca gtggcgggct ggtttgcggc gccggccggc
    21001 gtcaacgaaa acgcgcctcc gggacgaatt gcaagttggt gtcgagcgag ccaacatagc
    21061 ccgctagcgc cttcaactcg gcattcgtga attgcttggc aatgcccgcc atgatggcgt
    21121 tggaacgacc aaagtaggca cccgaaccgt ccttgtactg cctgagggcc acgtacagat
    21181 agtcggcgta ctgcccggct atttgggggt aggaggggtc gatgggcttg gcgaagttct
    21241 cgccatggca ggacacgcag gcgccctttt gcagcagctg cgccacctgg gcattgggct
    21301 cgcgcgcggg ctgggcttgc agctctgtcg ccctgccatg gccggaatag taggcggcaa
    21361 catctgccat gtcctgctcg gtcaggctgt cggcgatggc gcgcatggag gggaacttgc
    21421 gctcgccctt tttataggcc tccagcgccg cggtgatgta cttcgcgtcc tgcccgccga
    21481 tcttgggcac cttgtagacc tctggaaaat tcgcctggta gcccgatatg ccatggcagc
    21541 ctatgcacat ggccaccttg ccggcaccgg ccttggcgtc acccttgggc tcctcggcat
    21601 ggagcaaacc ggtcgcacaa gcgacggaca ggccgaatac ggtggtcagt agttttttca
    21661 ttttgcgcgc acaatcaaac gattggttgt ctccgcgatc tctatcgcaa acgattatag
    21721 gcagcggctg tcaagccttt tcatcaacga tttccctatt accggcccat gaaattccaa
    21781 ggctctgaaa actacgtcgc cacgcaagac ctgatgctgg ccgtcaacgc cgccatcaaa
    21841 ctgcagcgcc cgctgctcgt caagggcgag cccggcacgg gcaagaccat gctggccgag
    21901 gaggtggcgc aggcgctgaa catgcccctg ctgcagtggc acatcaagag caccaccaag
    21961 gcgcagcagg gcctgtacga gtatgacgcg gtgagccgcc tgcgtgattc gcagctgggc
    22021 gacgagcgcg tcaaggacat ccacaactac atcgtcaagg gcgtgctgtg gcaggccttc
    22081 acggccgatg cgcccgtggc cctcttgatc gacgagatcg acaaggccga catcgagttt
    22141 cccaacgacc tgctgcgcga gctcgaccgc atggagttct actgctacga gacgcgcgag
    22201 atggtgcgtg ccaagcaccg gccgctggtg ttcatcacct cgaacaacga aaaagagctg
    22261 cccgacgcct tcctgcgccg ctgcttcttc cactacatca agttccccga ggccgagacg
    22321 atgaagaaga tcgtcgatgt gcactttccc acgctcaaga gcgagctgct cacggcggcc
    22381 atgaagacct tctacgacgt gcgcggcctg ccggggctga agaaaaagcc ctcgaccagc
    22441 gagctcatcg actggctcaa actgctggtg gccgaggaga tcccgctcga agccctgcaa
    22501 agcgcggaca acaaggtcag cgtgccgccg ctggtgggcg cgctgctgaa gaacgaacag
    22561 gacgtgagcc tgttcgagaa gctggtcttc atgaaccagc gcaaccgctg accggggccg
    22621 ccacatgagt gccacacaac gcttgctgct ggaaggtctg tccgacgccg tgggcttcgt
    22681 cggcggcgcg ctcgccggct atggcctggg cctgctgctg ggcatggaca tcttcgacca
    22741 gggctatggc tcggccagca tcctgggcat tgccctggtg ggtctgggcg gcgggctggg
    22801 cctgcacctg gcgcggcgct ggcgcgcagc gcgcatgacg gcaaggcagg gggcgcagcc
    22861 atgagctttg tcgagcccgt caccctgcgc cggggcggcc tgttgctgga gccgctggcg
    22921 ctgtcgcacg aggaaggtct gcgccaggcc gccgccgacg gcgagctgtg gcgcatacgc
    22981 gtgaccagcg tgccagagcc gcaggacacg cgcacctata tcgaggccgc gctgcagggc
    23041 cgcgccgagg gccagcgctt cgccttcgcc gtgatcgacg aggccagcgg ccgcgtgctg
    23101 ggcagcacca gctaccacga catattgccc gccgtgcgcc gcgtggagat tggctacacc
    23161 tggtacgcca agagcgtgca gcgcagccat gtcaacacca cggccaagct cttgatgatg
    23221 ggccatgcct tcgagcagct gcaatgccat gtcgtggggt ggcgcacgga caatttcaac
    23281 ttcgcctcgc aacgcgccat agaacgcctg ggcgccagga aagacggcgt gatccgcggc
    23341 catgcgctgc gccgcgacgg caccattcgc gacacggtga tgtacagcat gcgcgccggc
    23401 gaatggcccg aggccaaggc tcagttgctc tatcttttag agcggcatgc gcgcactgaa
    23461 taagcgccag aggccaattt aatgctgatc gattttttct acaccctgcg cgcggccaag
    23521 ctgcccgtgt ccgtcaagga gttcatgggc ctgctcgagg cgctgcaggt gggcgtggtc
    23581 ggcccccact ccgacgacgc ctggagcctg gacgactttt actacctggc gcgcaccgca
    23641 ctggtcaagg acgagaagca tttcgacaag ttcgaccgcg cctttgccgc ctacttcaag
    23701 ggcgtggaga tgctggccga tttcaccaag gaaatccccg ccgactggct gcgcaaaatg
    23761 ctcgagcgcg agctgacgcc cgagcaaaag gccgccatcg agaagatggg ctgggacgag
    23821 ctgatggaga cgctgaagaa acgcctggag gaacaaaagg gccggcacga gggcggcaac
    23881 aagtggatag gaacgggcgg caccagcccc ttcggccatg gcggctacaa cccgcagggc
    23941 atacgcatag gcggcgccgg caagaacaag agcgccgtca aggtctggga gcagcgcgcc
    24001 taccgcgact acgatgacca gcaggagctg ggcacgcgca acatcaaggt ggcgctgcgc
    24061 cgcctgcgca agtttgcgcg cgaagggcat gagctggagc tggacctgcc cgacaccatt
    24121 cgcagcacgg ccgccaacgc gggctacctg gacatcaaga tggtgcccga gcgccacaac
    24181 aacgtgaagg tgctgctctt gatggacgtg ggcggcacca tggacgagca tatccagcgc
    24241 gtggaagagc tcttttcagc ggtgaaaagc gagttcaagc acctggagtt ctattacttc
    24301 cacaactgcg tctacgactt catgtggaag aacaaccgcc gccgctttgc cgagaagttt
    24361 ccgacctggg acatcatccg caagtacaac aaagactaca aactgatctt cgtgggggat
    24421 gccaccatga gcccctacga gatcctgcag cccggcggca gcgtggaata caacaacgag
    24481 gagccgggcg ccgaatggct gcagcgcctg acgcatgcct tccccaagtt cgcctggatc
    24541 aaccccgagc cccagggcgt gtggcagtac cggcagagca tctccatcat ccagcagctg
    24601 atgggccaac gcatgtaccc gctgtcgctc aaaggcctgg aggacacgat gcggcttttg
    24661 tcaaaatagc ccccatgttc cacatgcgca caacgccggc ccggtggccg gcggtgcttt
    24721 ttctgaccct ggccctggcc gtactgccgg cctgcggcac gctgcagggc gcgcctggcg
    24781 ccgaaccctc cgaaccccag gccgagcgcg acgccttcac gctcgacatc caggccgagg
    24841 atgccgccat ccgcgactat cttggccggc acctggagct gcagcgcttt cgccagctgc
    24901 ccgacctgca atcccaggag ctggcgcgcc tgctgcaggt agcagacgcc aacgcgcgcg
    24961 agctgctggc cacgctgggc tacttcagcc ccgagatcac gctgcagctg caggacaagc
    25021 ccacgccggc caaagatgaa cccgcgcaga ccgtgcgcct ggcggtacaa cccggcccac
    25081 gcacgcaagt cgagagcgtg gacattgcca tcgcccatgc cgacaccccc gacctcaagc
    25141 gccgccaggc ccagctgcag cgcaactggg gcctgcccga gggcgagccc ttcacccagc
    25201 aggcctggga cgaggccaag gcccagggcc tgcgcagcct gcaggcgcgc cgctaccccc
    25261 aggcgcgcct tgtggacagc cgcgccgaca tagccgccga cagcgcccag gcgcgcctgt
    25321 cggtgcagta cgacagcggc cccgcctacc gcttcgggcc gctgcagatc cgcggtggcg
    25381 agcgctacga cagcgtgggt ctggcccgca tcgccctgct gcccacgggc caggactacc
    25441 gcgaaggcca gctgctcgac gcccagcagc gcctggccgc cagtggctac tacgacgccg
    25501 tgttcctgac gctggacagc gacgcccccg acccgcaggc cgcgcccgtg atcgcccagg
    25561 tgcgcgatgc gccgctgcaa aaactggtgt ttggcgtcgg catctccacc gacagcggcc
    25621 cgcgcctgtc catggaccat agccacaacc ggttgccggc cttaagctgg cgcgcggtca
    25681 gcaagctggc actggacaac aagcgccggc tcgtctccag cgactggacg gccctgccct
    25741 cggccagcgg ctggcgctgg ttcagcggcg cacagctgca gcgcgagagc accggcagct
    25801 tcgaggtgaa cagcacacgc ctgcgcgccg ggcgcacgca aaacaccgac cacatcgacc
    25861 gcagctatta cctgcagcac gacaccgccg acagccaggg cataggggcg ccggcctcca
    25921 gcagcgccat cagcgccaac ttcggctgga cggggcgcta cttcaacaac aataacgccc
    25981 ccacgcgcgg ctggggcctg gccggcgaga tcggcctggg cagcacgctg cggcccacgc
    26041 gcgagccgtt catgcgcacg cagctgcgct ggcagtcctt cattgacctg gggcgcgtgg
    26101 atctgggcaa caaggtgcgc cgcgccagcc gcctgtcgct gcgcgtggag ggcggcgccg
    26161 tgctggcgcg cgccgatgcc gacattcccg tgacccagct gttcctgacc ggcggcgaca
    26221 ccaccgtgcg cggctatggc taccgcagca taggcgccgg catcaacaac ggccagacct
    26281 atggcggccg ctacatgacg gtggccagcg ccgaatggca gcgccccatc gtgctgcgcg
    26341 gcaaccgcac cgactttgaa agcgccgtgt tcatcgacgc cggcgccgtg gccgacaagc
    26401 ccggcgagct gagcgcccgt gtcggcctgg gcgccggcct gcgctggcgc agccccgtgg
    26461 gcccgctgca ggccgacctg ggctacggcc tgcagtcgca cgagctgcgc ctgcacctgc
    26521 gcctgggcta taccttttga caatgagccc cggccaccac cccaacccct tcgccgcagc
    26581 tgctgcgcca cgcccgcagc ccacaccgcc gcgccgcagc caccgggtgc tgctggcgct
    26641 ggagctgctg ctggccacgg ccctggcctt gctgctggcg ctgtggtggt gggccggcag
    26701 cagcggctcg ctggccagca cgctgtcgcg cgccacgcac tacctgcccg aggggcagag
    26761 cctcgaaacc cgcgaggtga cgggctcact gcgcgcgggc gggcgcatcg gctggctgcg
    26821 ctggagcagc cccacgctga ccgtcgaggt gcaggatgca cgaatcgcct ggcaactggc
    26881 tccattgcta cgcaggcagc tacaattttc agagcttcag gcgacacaga tcaccctcac
    26941 accacagggc gaagccagca gcgaaccgcc cgagccgctg cagcaactgc tgctgccgct
    27001 gcaggtggag ctgccctttg cctttgagcg catcacctgg gccggcgcca gccccgtcac
    27061 catcgaaggc ctggccggca actaccgcta cgacggcacg cagcaccgac tggaggtgca
    27121 gcaactcaac ctggcccagg gccgctatgc gctgcaggcc caactgcagg cacgcgcccc
    27181 catggccctg acggcccagc tggacggcag cgtgcacacc gccttgcccg gcagcacaac
    27241 gcagctgcag gcgcaggcca gggcacagct gcagggcagc ctggcaagcg ccgccgggcg
    27301 cctgaacgtc acggcgcagc tgcgcggcac aggcaccgaa gcccgcccgc cactgcacgc
    27361 cgacctgcag gccagcctgg cgccctgggc cggccaaccg ctgctgcagg ccaccgccga
    27421 actgcaggcg ctggacctgg ccgctctgtg gccccaggcg ccgcgcacgc ggctcaccgg
    27481 cagcgccggc ctggagcccg cagacaacgg ctggcggctg cacgccgcgc tgca
//
LOCUS       DAOMTQ010000018        27498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_158_length_27498_cov_27.243924, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000018 DAOMTQ010000000
VERSION     DAOMTQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 27498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27498
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_27498_cov_27.243924"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..891
                     /locus_tag="PK518_03335"
     CDS             112..891
                     /locus_tag="PK518_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM47972.1"
                     /translation="MNLKHGSTPWVFIFLLQWLWAGCALAQLPDAVQRVKPSVVLVGT
                     YKATDNPRFRFVGTGFVVGDGLRVVTNAHVTKAAGGQEADAPALVVQVRSGASAWTLR
                     QARVLENNHEQDLALLQIDGPAVAALSIGASDQVREGEDFAFMGFPVGGLLGFSSVTH
                     RAIVSSITTMALPSPTSQQLSERAIRSMRSGPQQVFQLDATAYPGNSGGPLFDPRSGA
                     VFGVISMVLVKGTRESALSQPSGITYAIPSRYVSEMLARHP"
     gene            complement(1007..1276)
                     /gene="infA"
                     /locus_tag="PK518_03340"
     CDS             complement(1007..1276)
                     /gene="infA"
                     /locus_tag="PK518_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRM47973.1"
                     /translation="MSNKEELIEMQGKVDEVLPDSRFRVTLDNGHALIAYSGGKMRKH
                     RIRVLAGDAVSVEMSPYDLTKGRITFRHLPPRAGGAPRAPARPRR"
     gene            complement(1760..2422)
                     /gene="alkB"
                     /locus_tag="PK518_03345"
     CDS             complement(1760..2422)
                     /gene="alkB"
                     /locus_tag="PK518_03345"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HRM47974.1"
                     /translation="MPHQPLDDLFADDSAQPTALPLGTQAVLLRGFALAQADALVQGV
                     QQVAAQAPWRHMLTPGGQAMSVAMTGCGPLSWVSDRRGYRYAERDPVSGRPWPAMPAA
                     FMRLAQDAARQAGFAPFLPDACLINRYAPGARMSLHQDRDERDLAAPIVSVSLGLPAV
                     FLWGGATRSERALRVPLRHGDVLVWGGVDRLRFHGVLPVAPGEHPATGQCRINLTLRK
                     AL"
     gene            2505..4013
                     /locus_tag="PK518_03350"
     CDS             2505..4013
                     /locus_tag="PK518_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM47975.1"
                     /translation="MNIAQLLQRSALVHGDRPAVLAGARLLHDYRGLAARVAALAGHL
                     RQRCGVQPGERVAIYSANCPEYLEALHAIYWCGAVSVPVNYKLHARELAHVLGDSGAR
                     VVCVSAELRDAAFAAGADPAALQVLGGPGWSQAAQGAPLAMEERTPEDLASLFYTSGT
                     TGRPKGVMQTQRNLLAMTMAYFTDVDDVHPDDAMVYAAPMSHGAGLYNYAYMLRGARH
                     VLPESGGFDPAELAQLAADVGRLSLFAAPTMVHRLVEHLRATGGDGSGFKTIVYGGGP
                     MYVDDLRRALDTMGQKFVQIYGQGECPMTITALAREHLMDTRHPRWEQRIASVGVAHA
                     CVEVRVADANDEPVPQGELGEVLVRGDTVMAGYWNNPEATAQTLRGGWLHTGDVGSLD
                     ADGFLTLQGRSKDVIISGGSNIYPREVEEVLLLHPRVSEAAVVGKQDAEWGEVVIAFL
                     VADGEPVPDAELDALCLAHIARFKRPKQYRWVPALPKNSYGKVLKTELRAWL"
     gene            4129..4845
                     /locus_tag="PK518_03355"
     CDS             4129..4845
                     /locus_tag="PK518_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM47976.1"
                     /translation="MATYTVKLLAKEPVAQGTMAFHLERPAGFEFRAGQAFEVILPGG
                     PEGEDGSHAFSIVTAPHEAELMFATRMRDSRFKRALGALPLGAELNIDGPFGSLTLHK
                     NPARAAVLIAGGIGITPFMSMLRSAAEQQSQHKLVLLYSNRRPEDTAFLAELQALVKR
                     NPRFHLMATMTDMGHSQQPWEGSTGIIDSTFVQHAIAGLDAPIFYVSGPPALVEAMRN
                     TLVQAGVDEDDVRSEEFFGY"
     gene            complement(4858..6423)
                     /locus_tag="PK518_03360"
     CDS             complement(4858..6423)
                     /locus_tag="PK518_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47977.1"
                     /translation="MANATAPFHPIIYVRGYAMTEREQDETTADPFCGFNLGSTVYRA
                     TADKNQPAKKFVFESPVLRLMSDYGYADIYDNGLDIMDGEWEGRISTRSIIIYRYYEQ
                     ASALLGSSKTPDISHFARGLSELILRVRDLACANPDNQLAPEDFRCYLVAHSMGGLVC
                     RALLQNPGLGDDQARGCVDKFFTYATPHNGIDMAGINVPQWLSAGDMNNFNRQNMAGY
                     LKLEALYAKTGRVDWLPEKAFASERVFCLIGTNRADYGVAMGLSRSFAGHGSDGLVRI
                     ENASLWGVNAKGKLSAPCATAYAYRSHSGYFGIVNSEEAYQNLTRFLFGDVRVDIWAD
                     IDGVQLPPEIEGKTVNALYQFEVLGSPRGKRWYLTRRMAEEDSVACRSHQELTDPTNK
                     DARSIYLSTVFLANRARVNPVRPSLAYGLTLGVRVPDYEVERKFWADGHYEGGYLFHD
                     TVIVEMVPPQQPGEAWTASFGWQSDNVGRATTPLSYKTLKTGKQVMSIAFGAARAPSI
                     TGRLRFVVSAWNA"
     gene            complement(6440..7048)
                     /locus_tag="PK518_03365"
     CDS             complement(6440..7048)
                     /locus_tag="PK518_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM47978.1"
                     /translation="MRIEEMIAAIQAQLGLDVDGKAGPQTWGAIYARIVQKKIDGQPP
                     ALAIATVDARSEKAIATLLPQVQPMARALVQKAAAAGITIRVLSGLRSFAEQDALYAQ
                     GRTAPGPIVTKARGGYSNHNFGIAFDVGVFEGNRYLADSPKYKAVGALGMDLGLEWGG
                     SWKTIVDQPHFQLRPAWAMDLSERDMLAEMRSRQAAGGPVFA"
     gene            7162..7830
                     /locus_tag="PK518_03370"
     CDS             7162..7830
                     /locus_tag="PK518_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRM47979.1"
                     /translation="MTPATPTLLKIDFVSDIVCPWCAIGLAALAQAAERLAGQVRLDW
                     HFQPFELNPGMGPEGEDLFEHLAAKYGSTPEQLRQMHAQIGARGAALGFTFSERRTRI
                     YNTFDAHRLLHWLEVEGTGEQRLALQRALFASYFTDGENPGEHAVLLRLVARLGLDEA
                     RARAVLQSQEYTDAVRQQQAFYQGQGIHSVPAVIINERHLIQGGQPVEVFEQALRQIA
                     AQQA"
     gene            complement(7902..8381)
                     /locus_tag="PK518_03375"
     CDS             complement(7902..8381)
                     /locus_tag="PK518_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HRM47980.1"
                     /translation="MRALRATALLALALAGSAQAADAEKIGTVDTAFQWIGRDHDIIV
                     EAYDDPLVAGVTCYVSRARTGGIKGTLGLAEDRAEASIACRQVGPITFAQPLRSQQEV
                     FSERTSLLFKRLRVVRMVDARRNTLVYLTYSEKLIDGSPQNSITAVPVDRATPIPLK"
     gene            complement(8378..10972)
                     /gene="cphA"
                     /locus_tag="PK518_03380"
     CDS             complement(8378..10972)
                     /gene="cphA"
                     /locus_tag="PK518_03380"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRM47981.1"
                     /translation="MQITRIRALRGPNLWTRSTAIEAIVQCAPEEHDISQLPGLEDRL
                     RARFPQIGALQPQGAGQSVSLADVLEAAALSLQAQAGCPVTFSRTHATVEEGTFQVVV
                     EYTEESVGRLAMERAEQLLQAALADTPFDADTVVAELRELDEDDRIGPSTGSIVDAAV
                     ARGIPFRRLTSGSLVQLGWGSKARRIQAAELDLTSAVAESIAQDKDLTKRLLHAAGVP
                     VPMGRPVADVDDAWAVAEEVGLPVVVKPQDGNQGKGVTVNITTRAQLEAAFATAQSFS
                     DEVMVERFLPGNDFRMLVVGHQLVAAARRDPPQVLGDGQHSVRELVDIVNQDPRRGSG
                     HGTALTKIRLDDIAIARLASEGLTPESVPVQGQRVVLRNNANLSTGGSATDVTDDVHP
                     EIAARAIEAAQTIGLHICGVDVICESMLKPLEEQGGGIVEVNAAPGLRMHLAPSFGRP
                     RNVGVPMVDELFAPGEDGRIPVVAVTGTNGKTTTTRLITQLLAAHGLRVGMTNTDGVY
                     VHGRQIDSGDCSGPKSARNVLFHPEVDAAVLECARGGVLREGLGFDRCQVAVVTNVGE
                     GDHLGLNFITTVEDVAVLKRVIVQNVAQDGYAVLNAADPHVAAMAGNCPGQVIFFAQD
                     RHHPVMATHRAQGSRVVYVDDGHIVAAEGSWRESIALRDIPITRGGTIGFQVENVMAA
                     VAASWALGLPWQTIRRGLAGFVNDGDNAPGRFNVMDYRGATLIADYGHNPDAMRALVQ
                     AVAAMPGQRRSVVISGAGDRRDQDIIEQTRILGAAFDDVVLYQDACQRGRADGEVLAL
                     LQQGLQGAPRTSYVTEIHGEFIAIDHALARLQPGDLCLVLVDQVHEALEHLRQRCAET
                     SSATAGAA"
     gene            complement(11053..13218)
                     /gene="cphA"
                     /locus_tag="PK518_03385"
     CDS             complement(11053..13218)
                     /gene="cphA"
                     /locus_tag="PK518_03385"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRM47982.1"
                     /translation="MANFKDIQLLRTTFLRGPSVWTYRPILEVWLDLGELEDFPSNKL
                     PGLNERLTAWLPDLVEHTCGVGERGGFIQRLEGGTWMGHVLEHVVIELLNLSGMPAEF
                     GQTREISRRGIYRMVFRCPEEAVARVALEHGHRLLMAAINDQAFDLKAAIQAIKTAIN
                     DRYLGPSTGCIVDAAGERRIPHIRLNDGNLVQLGYGAAQRRIWTAESDQTSAIAEGIA
                     QDKDFTKRLLASCGVPVPEGQIVNSAEEAWEVAQDIGFPVTVKPSDGNHARGVTLELS
                     QEADIKAAFALAQPEGSDVIVEKFIDGIEHRLLVVGGKVVAATKGETVSVHGNGQATL
                     AELVAVLNQDPRRGPEQEYPLDWIDTDKGAVQLELKRQNVTPATVIPKGQSVLLQRNG
                     NMAIDCTDDVHPDVAYYATLAAKIVGLDIAGMDMIVKDVSKPLQGQGAILEVNAGPGL
                     LMHLKPTSGAPRPVGMAIADHLFPREDGPGAGRIPLVGIVGTRHNAFIARLVGWLLQL
                     SGKLTGVASSEGMFIAGRQTQKANAAHWAGAHRLLTNRLAQAAVIQTTARSILEEGLA
                     YDRCLVGVVTDMDGYEGLADHDVLEQAKMTRVLRTQIDVVLDEGAGVLNADIAQVAEL
                     APLCDGEVLLYSTSADNAAVAAHRAEGEGRAVFVRAGQVILATGASERVLGSLQALSL
                     PGGQQPDTTALLAAIATAWSMHIGPDLIAAGIKTFEYQI"
     gene            13444..15753
                     /locus_tag="PK518_03390"
     CDS             13444..15753
                     /locus_tag="PK518_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM47983.1"
                     /translation="MQHQPSVDASGVLPGPLGAELRAMLAPDENVQAAFEVDLTPELR
                     FAPGLLVLSDRRLLARAMDGATQQWPLAPGMVLRMLDHGGVGTLELHDDQQRLALWRF
                     TLGRHPQALHLQQRLEQQIAHLQNPQALAVQRDEAPCCAVCGTPLPAGSDECPACARQ
                     QAKPTSTWVLLRLWRFARPYKKQLAAGFGLTLASTAASLVPPYMTIPLMDDILIPYQS
                     GKAIDPWLVVMYLGGLLLAALLAWGLGWARTYILALVSERIGADLRTTTYEHLLRLSL
                     DYFGSKRTGDLMARIGSETDRINVFLSLNALDFATDVLMIAMTSAILFSINPWLALVT
                     LVPLPFIAWLIHNVRDRLRTGFEKIDRVWSEVTNVLADTIPGIRVVKAFAQESREAQR
                     FRAANQHNLEVNDKLNKTWSLFAPTVSLMTEVGLLVVWGFGVWLVAHNQITVGVLAAF
                     IAYIGRFYTRLDSMSRIVSVTQKAAAGAKRIFDILDHVSNVPDPQNPVRVDKVAGAIE
                     MQGVGFRYGSRTVIKNLSLQIRPGEMIGLVGHSGSGKSTLVNLISRFYDVSDGAILVD
                     GVDIRRMAVSDFRRHIGLVLQEPFLFFGTIAENIAYGKPDATRAEIVAAARAAHAHEF
                     ILRLPHGYDSLVGERGQGLSGGERQRISIARALLIDPRILILDEATSAVDTETEKEIQ
                     KALDNLVQGRTTIAIAHRLSTLRKADRLVVMDRGEVVEVGPHDELMAQQGAYWRLYEA
                     QARRAEEDAEAAGVRIESQRQHSAHPAGK"
     gene            15765..16250
                     /locus_tag="PK518_03395"
     CDS             15765..16250
                     /locus_tag="PK518_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HRM47984.1"
                     /translation="MNTTTEPMAFDLRRNPHGRLVLRLADGTEHEAVTPVRAFPIAAP
                     QEGLSLVGADGHELAWVPRLADLAAAARTLIEEELAAREFVPTITRIRSVSSFSTPST
                     WEVDTDRGAAQLVLKGEEDIRRLSGRTKLLIAASDGLHFHVPDSQALDRASRKLLERF
                     L"
     gene            16403..16849
                     /locus_tag="PK518_03400"
     CDS             16403..16849
                     /locus_tag="PK518_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47985.1"
                     /translation="MQLPPVDRSPAQWRPQGADLYSTGASGAVPVRPVNAANPVESTD
                     RLGEGSVLREPTKPSAPDEENRDWTLPIEKKDKPSEADAPPKDPPLHQVMLEFIQSMW
                     RASASAVDVAQDIHKTTLQELQAQKAEDEQQSLVYSDPKVKRTGGL"
     gene            complement(16911..17828)
                     /locus_tag="PK518_03405"
     CDS             complement(16911..17828)
                     /locus_tag="PK518_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRM47986.1"
                     /translation="MAPSLSDKLVVAISSRALFDFEEENRVFEHGDDRAYVELQRQRL
                     DIPAPPGVAFSLVRKLLAFNHAEHQRVEVVLLSRNDPVSGMRVFRSCQAHGLPSVQRG
                     VFTQGRDPFRYLRPLGAHLFLSANETDVRSALHLGFPAARVLTESVQAGDAHPREVRI
                     AFDGDAVLFSDEAERVYQSEGLAAFQRHESAKAAQPLPEGPFKPLLAALHRLQQAGGA
                     AMRIRTALVTARSAPAHERAIRTLMDWNIAVDEAMFLGGLPKGEFLREFEPDFFFDDQ
                     TGHVNSAARHVPAGHVSSGIANAPAEIAK"
     gene            complement(17985..19214)
                     /gene="gspF"
                     /locus_tag="PK518_03410"
     CDS             complement(17985..19214)
                     /gene="gspF"
                     /locus_tag="PK518_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HRM47987.1"
                     /translation="MPAYSFEALDAQGHVRRGTLDADSPKAARNQLRAQALVPLDVEA
                     LSPEAATGSAPGLAQRLFTRPVFNATQLSIWTRQLAGLVGSGLPLERALTALADEADE
                     ERQRHLVAALRAEVNAGSTFARALAQHPREFSDIYCAVIGAGESSGGLSQVLDSLADD
                     LEARQQLRAKLIGAALYPAIVTLVAIVIVLFLVGYVVPQVASVFAGTKRALPLLTVIM
                     LAISDFVRGYGWAMLGFIVLIAVGARAALARPVFCQKFDAAWLQLPLVGKLARGYNAA
                     RFAGTLAMLTGAGVPILRALHAAAETLNNRALRADAQEALVLVREGAPLASALAQKKR
                     FPGLVAMFARLGEQTGQLPTMLQRAATQLGTEVQRRAMQLATILEPLLIVTMGLIVML
                     IVLAVLQPIIELNQFVK"
     gene            complement(19238..20242)
                     /locus_tag="PK518_03415"
     CDS             complement(19238..20242)
                     /locus_tag="PK518_03415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRM47988.1"
                     /translation="MTTNHTPVLSMLDLVAVREGGSVGDALAIAVRTAQHVESLGFKR
                     YWLAEHHNMPGIASSATAVLVGHIAGSTSHIRVGSGGVMLPNHAPLVVAEAFGTLAEL
                     YPGRIDLGLGRAPGTDGATMRALRRDRVETEADFPRDVAELQQLLAPAQPGQRLIATP
                     GAGTQVPLWLLGSSLFSAQLAAHMGLPYAFASHFAPRLLQPALQLYRELFRPSAVLHK
                     PYVMIGVPVIAAPTDEEAEYLASSTYQRVLGILTGQRGLLRPPVEGFLQGLPAPERAA
                     IGDFLAMGVVGGPDTVRAGLQALARDTRADEFMLVSDVFDPALRLRSLTLTAQATIAG
                     "
     gene            20431..20940
                     /locus_tag="PK518_03420"
     CDS             20431..20940
                     /locus_tag="PK518_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HRM47989.1"
                     /translation="MGFFSRLFQPRPDGLQTHSDWSRLPDTDASELVLFDDEASKLMA
                     QFDIDAAIVTHERWLPWLGEVLQGARDERLRPEVVADDGCSELGQWLHGSGRTALGHY
                     PAFDMLLRRHRYFHQQAAALITHAEAGDILMAQQAHKACQHASRQVVLLLKELQKGLL
                     RTRRPVLGR"
     gene            complement(21024..21410)
                     /locus_tag="PK518_03425"
     CDS             complement(21024..21410)
                     /locus_tag="PK518_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47990.1"
                     /translation="MNTITRLSAPLLPLLLTACAATAPPARDFPAGVRTPSAAELAAL
                     LRGKSTRTPMPDGSTVRADYAAEGNHLTIYAAGRSDTGTWLAEDGRVCFEFKVFRSAC
                     NDIRLAGQDIYAKRANGDVVPVTVGR"
     gene            complement(21475..23127)
                     /locus_tag="PK518_03430"
     CDS             complement(21475..23127)
                     /locus_tag="PK518_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM47991.1"
                     /translation="MRYTNGRRLPYSLTIIAALAMAACGGSSSDDTDGGGGDGGGGGS
                     GASITLSGEVARLGTLKNVIVCLDLNGNDACDSGEPASSATGADGKYSLSTTASQAAA
                     ARLIAPVKTGDPAAATTAIDSHNPAVAATDADYVLKRPAGSGGAINPLTTLAQAGVAA
                     GMTEADARANVALQLAIGAAKIDNYQDDPAWDDAQVRDTARTAAAVVSGMLRQGIALE
                     VGNQQAAAPASTILRQLNYSGSGDYYLRAYDRQARAAGTAEEQSVDARSGKSGGVDMT
                     EDSLYGNAFLTPSGWKYCDRRVLLRSTLGNPNRAVYCDGQTTLGWDRANSVAGEAMAT
                     LVNRWQAMPSNTINAGIPTAALTGKLGAAAFPAGAEEWIRTNVELAPSITITSVWSRR
                     LPQSRNTLEAVIAYYPTSGAASPTGANTLNLALTSSALKNLRVSFAPTDATQGTATYY
                     ECDLNEAQTVVSNCAAIAAKGSYSIDTINGARVLRFAGQPPTPALNYHVAYAEIQWTA
                     GDPSSRWVYRAHEVKPSLSARQSTTNRLNETAWAAMKVQLGL"
     gene            complement(23329..23979)
                     /locus_tag="PK518_03435"
     CDS             complement(23329..23979)
                     /locus_tag="PK518_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM47992.1"
                     /translation="MRILLVDDHALFRDGVALLLKELDPAVQTVHAAHLGEALAALDH
                     DTGIDLVLLDLILPGANGFQALETIRERHPGMPLVMLSSQEDQVTVLRAIQLGAMGFI
                     PKSASSQQMFEALRTVLAHGIHIPPLSLAGIALTVPAQPRTSPAELGLTPRQTQVLRL
                     ILEGKPMKLIARELGLSHNTAKTHVSAVLRALCVTTRTQAIVAASRMGLVLGRDDQ"
     gene            complement(23957..26266)
                     /locus_tag="PK518_03440"
     CDS             complement(23957..26266)
                     /locus_tag="PK518_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM47993.1"
                     /translation="MRPSGPRLWQTYALTVVALVALPLTASGLAQLLLIQRETRVAVQ
                     AVQALEAAKASAKIERFISQTAALLRTTLSLQDSRHLGNADADIQYELYRLMKAERAI
                     QTLYWIDPHGQERVRISRMAPDRIGGGEDRSQSPEFLHARADAPWFGPVTFAVNLPRV
                     VIARRGRLPESGVLAAEVELSELRDIIHETRFGSDGVAYVVDGQGRLLAHPDFLRVLT
                     LTDWATRPPVRTAGRGGLIEFETPEGPMVARVAELSVPGWRLITEQPRAEAYAPVRRA
                     LATALLILVLAVLTGSGVGIVLARRVVRPIQQLAEGADRIGEGQLDHRIELARSDELG
                     QLADRFNQMAERLGDSLRLLETRVAERTQDLAKANALLQARREEAEQASQAKTRFLAS
                     ASHDLRQPLHTISLLVGVLRRQEVTPQVGQLVGHIQSSISAMEALFVGLLDISRLDAG
                     MSRPVLGDHALAELLHRVAASHAPEAEHKRLRLTVVPSRCAVRTDAVLLERVLANLVS
                     NAIRYTERGRVLVGCRRRPGAIELQVWDTGIGIAPAQLPHVFDEFFQVGNPERDRSKG
                     LGLGLAIVKRTLDLLGHPHTLRSAPGRGSRFSILLPAAEALPVAAPLPPALASGRRIA
                     GAFIAVIDDEADTRRAMQALCHSWGAHVLIAESTRQCLALLDEHLRYPDLILCDYRLG
                     GSHDGLGAVQRIRARIGHAVPAIIITGDIGAADLRRVADAGLPLLHKPAGTDRLLAAI
                     EAALNDPPTGGGATGQNGADCDHEDPAGR"
     gene            complement(26266..27189)
                     /locus_tag="PK518_03445"
     CDS             complement(26266..27189)
                     /locus_tag="PK518_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRM47994.1"
                     /translation="MNQVRRALQESGWSFDGQLALEWNDAGNDPAQLTPMAQAIVASR
                     PDILIATENMPNEALMKATQKLPIVVMGPTNLHHVLDAQLRPLANVTGVSLGLNNQYA
                     IKPVEVLLQAFPTARRIGMMENNDQPGHNDGGSVGPITDMVRQIGAELVRVKFSGEAG
                     IASAWDELAGRKVDAVMIRPDTSAFLGEHARQAQRVGMPAIAHNSWFATRYGGLLSYG
                     AVGRVNMCGRGARYIDQVLRGRPLAELPIEELYEAALVVNLDAAQRLGVTLPPALIAR
                     ADRLIQPGERTLAPRADATPLPPFQPPARAR"
BASE COUNT         4226 a         9122 c         9608 g         4542 t
ORIGIN      
        1 ctgccgccgg ggacaagttt cccggccagg ccgaggtggc ggagttgaag aaggcactgt
       61 gagaggcgcc cctcggtgtt tgctgcaaca ggctggaggt tgatcagtgc catgaacttg
      121 aaacatggat cgacgccctg ggtgttcata ttcctgctcc agtggctgtg ggcgggttgc
      181 gcgctggcgc aactgcccga tgccgtgcag cgggtgaaac cctccgtagt gctggtcggt
      241 acctacaaag ccaccgacaa cccgcggttt cgttttgtcg gaacgggctt tgtggtgggt
      301 gatggcttgc gtgtcgtgac caatgcccat gtcaccaagg ccgctggcgg ccaggaggct
      361 gatgcaccgg ccctggtggt gcaggtacgc tcaggcgcaa gcgcctggac gctgcgccag
      421 gccagggtgc tggaaaacaa ccacgagcag gatctggcgc tgctgcaaat cgacgggcct
      481 gcggttgccg cgctcagcat tggtgcatcc gaccaggtgc gcgaagggga agatttcgcc
      541 ttcatgggct ttccggtcgg cgggctgctg ggcttttcgt ccgtcaccca ccgggccatt
      601 gtgtcgtcca tcaccaccat ggccctgccc agcccgacca gccagcaatt gagcgagcgc
      661 gccatccgca gcatgcgctc ggggccgcag caggtgtttc agttggacgc cacagcctac
      721 cccggcaaca gcggcggccc gttgttcgat ccccgtagcg gtgccgtgtt tggcgtcatc
      781 agcatggtgc tggtgaaggg cacgcgcgaa tcggcactca gccagccttc gggcattacc
      841 tacgccattc caagccgcta tgtgtcggag atgctggcgc ggcatccatg agtcattcca
      901 tttctaaatc acccgtgtcg ttgttgcttc gccttgtcgt gctacagcac tgcctgcggc
      961 tccgcgccta gacacgaatg atttggaagc ggaatcaaat acggtactag cgccgtggac
     1021 gcgccggagc gcgcggcgcg ccgcccgcgc gcgggggcag gtggcggaag gtgatgcggc
     1081 ccttggtcag gtcgtagggc gacatctcca ccgacaccgc gtcgcccgcc agcacgcgga
     1141 tgcggtgctt gcgcatcttg ccgcccgagt aggcgatgag cgcgtggccg ttgtcgaggg
     1201 tcacgcgaaa gcgtgagtcg ggcagcactt catcgacctt gccttgcatc tcgatcagtt
     1261 cttccttgtt ggacatgggg ttcctctttt ttgtcagggg cgagtgatgg cgggcgcgac
     1321 ccatggccgg cgccacccgc actcgatggg ggtctgtgtg caatgcacag aaaacgcatg
     1381 cgccggcaac cccggcgcgg gatgggcgat ggcgcatcgc ccgtgcaaga cgccggtatg
     1441 caacgctggc atctactggt aaggccgctg cggccgggca tgctgccccg gactgcgggt
     1501 gggcagggtg cccagcgcgg cggtgatgtg tgcgggactg gcctgcacag gctgtgctga
     1561 cgaagcgcgt gcccggcagc aaaggggcgc agtctagtgc gtaatggact tttgtgctgg
     1621 cgccaaggtc attggccctg ccgcgggctg tggcatggcg ctggccatgt cttggtgcag
     1681 ggtgtggcgt ttgggcctac ggttgaaaag gcctgcaggt gttttacagc aaacgctcga
     1741 tgctatcgtt tttgtggttt caaagcgcct tgcgcagcgt caggttgatg cggcactggc
     1801 ccgtggccgg gtgctcgccc ggcgccacgg gcagaacgcc atgaaagcgc agcctgtcca
     1861 cgccgcccca gaccagcacg tcgccatgcc gcagaggcac gcgcagtgcg cgttctgagc
     1921 gcgtggcgcc gccccacaga aagacggccg gcaggcccag cgataccgag acgatgggtg
     1981 cggccaggtc gcgctcgtcg cgatcctggt gcagcgacat gcgcgcgccg ggtgcgtagc
     2041 ggttgatcag gcaggcatct ggcaggaagg gcgcaaaccc cgcctgccgc gcagcatcct
     2101 gcgccagtcg catgaaggcc gcgggcatgg ccggccaggg ccggccgctc accgggtcgc
     2161 gctccgcgta gcggtagccg cgccggtcac tgacccagga caaaggcccg cagcccgtca
     2221 tggccaccga catggcctgg ccgcccgggg tgagcatgtg acgccagggc gcctgcgcgg
     2281 cgacctgttg cacgccctgc accagggcgt cggcctgggc cagcgcaaag ccgcgcagca
     2341 gcaccgcttg cgtgcccagg ggcagggccg taggctgggc gctgtcgtcg gcaaacaggt
     2401 cgtcgagggg ttgatggggc atggcaggag tttgagccaa aatgccgtgt agcgcttatc
     2461 catcaagcgc aaacagctat ttttttaaga gtgaggagac ggcaatgaat atcgcccaac
     2521 tgctgcagcg cagcgccctg gtgcatggcg accgcccggc ggtgctggcc ggcgcgcgcc
     2581 tgttgcacga ctaccgtggc ctggcggcgc gcgtggcggc gctggccggg catttgcgcc
     2641 agcgttgcgg tgtgcagccg ggcgagcgcg tggccatcta ctcggccaac tgccccgagt
     2701 acctggaggc gctgcatgcc atctattggt gtggcgcggt gtcggtgccc gtcaactaca
     2761 agctgcatgc gcgcgagctg gcccatgtgc tgggcgacag cggcgcgcgc gtggtctgtg
     2821 tgtcggccga gctgcgcgat gcagcgtttg ccgccggcgc cgaccccgcc gccctgcagg
     2881 tgctgggcgg ccccggctgg agccaggcgg cgcagggcgc gccgctggcg atggaggagc
     2941 gcacgcctga ggatctggcc tcgctgttct acacctcggg caccaccggc cggcccaagg
     3001 gcgtgatgca gacgcagcgc aacctgctgg ccatgaccat ggcctatttc accgacgtgg
     3061 acgatgtgca cccggatgac gccatggtct atgccgcgcc catgtcgcat ggcgccggcc
     3121 tgtataacta cgcctacatg ctgcgcgggg cgcgccatgt gctgcccgaa tcgggcggtt
     3181 tcgacccggc cgagctggcg cagctggccg ccgacgtggg ccggctgtcg ctgtttgccg
     3241 cgcccaccat ggtgcaccgg ctggtcgagc acttgcgcgc cacgggcggc gacggcagcg
     3301 gcttcaagac catcgtctac ggcggcggcc cgatgtatgt ggacgacctg cgccgcgcgc
     3361 tggacaccat gggccagaag ttcgtgcaga tctacggcca gggcgaatgc cccatgacca
     3421 tcaccgccct ggcgcgcgag catttgatgg acacccggca cccgcgttgg gagcagcgca
     3481 tcgcctcggt gggcgtggcg cacgcctgcg tggaggtgcg cgtggccgac gccaatgacg
     3541 agcctgtgcc ccagggcgag ctgggcgagg tgctggtgcg cggcgacacg gtcatggccg
     3601 gctactggaa caaccccgag gccacggcgc agacgctgcg cggcggctgg ctgcacacgg
     3661 gcgatgtcgg cagcctggat gctgacggct ttctcacgct gcagggccgc agcaaggacg
     3721 tgatcatctc tggcggctcc aacatctacc cgcgcgaggt ggaggaggtg ctgctcctgc
     3781 acccgcgcgt gagcgaggcg gcggtggtgg gcaagcagga cgccgaatgg ggcgaggtgg
     3841 tgatcgcctt cctggtggcc gatggcgagc ccgtgcccga tgccgagctc gacgccctgt
     3901 gcctggcgca tatcgcccgc ttcaagcgcc ccaagcaata ccgctgggtg ccggccttgc
     3961 ccaagaacag ctacggcaag gtgctcaaga ccgagctgcg cgcctggctg taaccatggc
     4021 gtggctgggc gctatgggtg cgcggctggc gtgatccggg cgcatgcggg aatatgggcc
     4081 ccatgcgccg catcccatgg tgcatgaccg tcattgagag gacacaacat ggctacctat
     4141 accgtcaagc ttctcgccaa agagcccgtg gcccagggca ccatggcctt ccacctggaa
     4201 aggccggcgg ggttcgagtt tcgcgccgga caggcgtttg aagtcatcct gcccggcggc
     4261 cccgagggcg aggacggcag ccacgccttc tccatcgtca ccgcgccgca cgaggccgag
     4321 ctgatgttcg ccacgcgcat gcgcgacagc cggttcaagc gcgccctggg tgcgctgccg
     4381 ctgggggccg agctcaacat cgacgggccg tttggctcgt tgaccctgca caagaacccc
     4441 gcgcgcgcag cggtattgat agcggggggc atcggcatca cacccttcat gagcatgcta
     4501 cgcagcgccg ccgagcagca gtcgcagcac aagctggtgc tgctgtactc caaccgccgc
     4561 cccgaggaca cggccttcct ggccgaactg caggcgctgg tcaagcgcaa ccccaggttc
     4621 cacctgatgg ccaccatgac cgacatgggc cattcgcagc agccctggga gggcagcacg
     4681 ggcatcatcg acagtacctt cgtgcagcac gccatcgccg ggctggacgc gcccatcttc
     4741 tacgtgtccg gcccgccggc tctggtcgag gccatgcgca acaccctggt gcaggccggt
     4801 gtggacgagg acgacgtgcg cagcgaggag ttcttcgggt attgaaagcg cgggtgctca
     4861 ggcattccac gccgacacca cgaagcgcag cctcccggtg atgctcggcg ccctggcggc
     4921 gccgaaggcg atgctcatca cctgcttgcc ggtcttcagc gttttgtagg acagcggcgt
     4981 ggtggcccgg ccgacgttgt cgctttgcca gccgaagctg gccgtccagg cctcgcccgg
     5041 ctgctggggc ggcaccatct cgacgatgac ggtgtcgtgg aacaggtagc cgccctcgta
     5101 gtggccgtcg gcccagaact tgcgctccac ctcgtagtcg ggcacgcgca cgcccagcgt
     5161 caggccatag gccagcgagg gccgcaccgg gttcacgcgc gcgcggttgg ccaggaacac
     5221 agtggacagg tagatgctgc gcgcgtcctt gttcgtcggg tcggtcaact cctggtggct
     5281 gcggcaggcc accgaatctt cctcggccat gcgccgcgtc aggtaccagc gcttgccgcg
     5341 cggcgagccc agtacctcga actggtacag cgcgttcacg gtcttgccct cgatctcggg
     5401 cggcagctgc acgccatcga tgtcggccca gatgtccacg cgcacatcgc cgaacagaaa
     5461 gcgtgtcagg ttctggtagg cctcctcgct gttgacgatg ccgaaatagc ccgaatgcga
     5521 gcgataggca taggccgtgg cgcagggcgc ggagagcttg cccttggcgt tcacgcccca
     5581 gagcgaggcg ttttctatgc gcaccaggcc atcgctgcca tggccggcaa agctgcgcga
     5641 caggcccatg gccacgccat agtcggcgcg gttggtgcct atcaggcaaa acacgcgctc
     5701 ggacgcgaag gccttttcgg gcaaccagtc cacgcgcccg gtcttggcgt acagcgcttc
     5761 cagcttcagg tagccggcca tgttctggcg gttgaaattg ttcatgtcgc cggcgctcag
     5821 ccactgcggc acgttgatgc cggccatgtc tatgccgttg tgcggcgtgg cgtaggtgaa
     5881 gaacttgtcc acgcagccac gtgcctggtc atcgcccaga ccggggtttt gcagcaaggc
     5941 gcgacacacc aggccgccca tggagtgggc gacgaggtag cagcgaaagt cctcgggcgc
     6001 cagctggttg tcggggttgg cgcaggccag atcgcgcacg cgcaggatca gctcggacag
     6061 gccgcgcgca aagtgcgaaa tatccggcgt cttgctgctg cccagcaggg ccgaggcctg
     6121 ctcgtagtag cggtagatga tgatggagcg cgtgctgatg cggccctccc actcgccgtc
     6181 catgatgtcc aggccgttgt cgtagatgtc ggcgtagccg tagtccgaca tcaggcgcag
     6241 caccggggac tcgaagacaa acttcttcgc cggctggttc ttgtcggccg tggcgcggta
     6301 cacggtggag cccaggttga agccgcagaa ggggtcggcc gtggtttcgt cctgctcgcg
     6361 ctcggtcatg gcgtagccgc gcacgtagat gatggggtgg aagggggcgg ttgcattggc
     6421 catggtgggg ctccagcggc tacgcaaaga ccgggccgcc tgccgcctgg cgcgagcgca
     6481 tttcggccag catgtcgcgc tcgctcaggt ccatggccca ggccgggcgc agctggaaat
     6541 gcggctgatc gacgatggtc ttccagctgc cgccccactc caggcccaga tccatgccca
     6601 gcgcgcccac ggccttgtat ttgggtgaat cggccaggta gcggttgccc tcgaacacgc
     6661 cgacgtcaaa ggcaatgccg aagttgtggt tggaatagcc gccgcgcgcc ttggtgacga
     6721 tggggccggg tgccgtgcgg ccctgggcgt acagggcgtc ctgctcggca aagcttctca
     6781 ggccgctgag gacgcggatg gtgatgcccg cggcagccgc cttctgcacc agcgcgcgcg
     6841 ccatgggctg cacctggggc agcagggtgg cgatggcctt ttcgctgcgc gcatccacgg
     6901 tggcgatggc cagggcgggc ggctggccgt cgatcttctt ctgcacgatg cgcgcgtaga
     6961 tcgcgcccca ggtctgcggg cccgctttcc cgtccacgtc aaggcccagc tgggcctgga
     7021 tggcggcaat catttcctcg atgcgcatgg tgtgctcccg gtgatgaggt gcggcctcca
     7081 tcaaaatacc gcggcgatgc cgtgtcaagc caagatttca ggctggcgga caatcgccgt
     7141 gatcgaccac cggaagcccg catgacgcct gccaccccca ccctcctgaa gatcgacttt
     7201 gtctctgaca tcgtctgccc ctggtgcgcc ataggcctgg cggcgctggc gcaggcggcc
     7261 gagcgcctgg cggggcaggt gcggctggac tggcatttcc agcccttcga gctcaacccc
     7321 ggcatggggc cggagggcga agacctgttc gaacacctgg ccgccaagta cggcagcaca
     7381 cccgagcagc tgcgccagat gcacgcgcag ataggcgcgc gcggcgcggc cctgggcttc
     7441 acgttcagcg agcggcgcac gcgcatctac aacaccttcg acgcgcaccg cctgctgcac
     7501 tggctggagg tggagggcac gggcgagcag cgcctggcgc tgcagcgggc gctgtttgca
     7561 agctatttca ccgatggcga gaaccccggc gagcatgccg tgctgctgcg cctggtggcg
     7621 cgcctggggc tggacgaggc gcgcgcccgc gccgtgctgc agtcgcagga gtacaccgac
     7681 gccgtgcgcc agcagcaggc cttctaccag ggccagggca tccactcggt gccggcggtg
     7741 atcatcaatg agcgccacct gattcagggc ggccagccgg tcgaggtctt cgagcaggcg
     7801 ctgcgccaga ttgccgcaca gcaggcttga aaatgcttct tttttaatag ctgttggcgc
     7861 tttttgatcg ggcgctgcgg gctgttttca cttcaaatca attatttcag cgggatcggc
     7921 gtggcgcgat ccacgggcac ggccgtgatg ctgttttgcg gcgagccgtc gatgagcttt
     7981 tcggagtagg tcaggtagac cagggtgttg cgcctggcgt ccaccatgcg taccacgcgc
     8041 aggcgcttga agagcagcga ggtgcgctcg ctgaacacct cctgctggct tctcaagggc
     8101 tgggcaaacg tgatcggccc gacctggcgg caggcgatgg aggcctcggc gcgatcctcg
     8161 gccaggccca gcgtgccctt gatgccgccg gtgcgcgcgc gcgagacata gcaggtcacg
     8221 ccggccacca gcgggtcgtc ataggcctcg acgatgatgt cgtgatcgcg gccaatccat
     8281 tggaaggccg tgtccaccgt gcctattttt tccgcatccg ccgcctgggc gctacccgcc
     8341 agggccaggg ccagcagcgc ggtcgcgcgc aacgccctca tgccgcaccc gccgtggcgc
     8401 tggaagtttc ggcgcagcgc tggcgcagat gttccagcgc ctcgtgcacc tgatcgacca
     8461 gcaccaggca caggtcgccc ggctgcaggc gcgccagggc gtggtctatg gcgatgaact
     8521 cgccgtggat ctcggtcacg tagctggtgc gcggcgcgcc ctgcaggccc tgctgcagca
     8581 gggccagcac ctcgccgtcc gcgcggccgc gctggcaggc gtcctggtac agcaccacgt
     8641 cgtcgaaggc cgcgcccagg atgcgggtct gctcgatgat gtcctggtcg cgccggtcgc
     8701 cggcaccaga aatcaccacg ctgcgccgct ggcccggcat ggcggccacg gcctgcacca
     8761 gggcgcgcat ggcgtcgggg ttgtggccgt agtcggcgat cagcgtagcg ccgcggtagt
     8821 ccatgacgtt gaagcgcccc ggggcgttgt cgccgtcgtt cacaaagccg gccaggccgc
     8881 ggcgtatggt ctgccagggc aggcccaggg cccagctggc ggccacggcg gccatgacgt
     8941 tctcgacctg aaagccgatg gtgccgccgc gcgtgatcgg gatgtcgcgc agcgcgatgg
     9001 attcgcgcca cgagccctcg gcggcgacga tatggccatc gtccacatac accacgcggc
     9061 tgccttgcgc gcggtgcgtg gccatcaccg ggtggtgccg atcctgggca aagaagatca
     9121 cctggccggg gcagttgccg gccatggccg ccacatgcgg gtcggcggcg ttgagcacgg
     9181 cgtagccgtc ctgggccacg ttctgcacga tgacgcgctt gagcacggcc acgtcctcca
     9241 cggtggtgat gaagttcagc cccaggtggt cgccctcgcc cacattggtg accacggcca
     9301 cctggcagcg gtcgaagccc aggccctcgc gcagcacgcc gccgcgcgcg cattcgagca
     9361 cggccgcgtc cacctcgggg tggaacagca cgttgcgtgc gctcttcggg ccggagcagt
     9421 cgccgctgtc gatctggcgg ccgtgcacat acacgccgtc ggtattcgtc atgccgacgc
     9481 gcaggccatg cgcggccagc agctgggtga tcagccgcgt ggtggtggtc ttgccattcg
     9541 tgccggtgac cgcgaccacg ggaatgcgcc catcctcgcc cggggcaaac agctcgtcga
     9601 ccatgggcac gccgacgttg cgcggcctgc cgaaggacgg cgccaggtgc atgcgcaggc
     9661 cgggggcggc gttcacctcg acgatgccgc cgccttgctc ctccagcggc ttcagcatgg
     9721 attcgcagat cacgtccacg ccgcagatgt gcaggcctat ggtctgcgcg gcctcgatgg
     9781 cgcgcgcggc gatctcgggg tgcacgtcgt cggtcacatc ggtggcgctg ccgccggtgg
     9841 acagattggc gttgttgcgc agcaccacgc gctggccctg cactggtacg gattcggggg
     9901 tcaaaccctc gctggccagg cgcgcgatgg cgatgtcatc caggcggatc ttggtcagcg
     9961 ccgtgccatg gcccgagccg cggcgcggat cctggttcac gatgtccacc agctcgcgga
    10021 cgctgtgctg gccgtcgccc agcacctgcg gcgggtcgcg ccgcgcggcc gccaccagct
    10081 ggtggcccac caccagcatg cgaaagtcgt tgccgggcag aaagcgctcg accatcacct
    10141 cgtcgctgaa gctctgcgcg gtggcaaagg cggcctccag ctgcgcgcgc gtggtgatgt
    10201 tcaccgtcac gcccttgccc tggttgccgt cctggggctt gacgaccacg ggcaggccga
    10261 cttcctcggc cactgcccag gcgtcatcca catcggccac cggccggccc atgggcacgg
    10321 gcacgccggc ggcgtgcagc aggcgcttgg tcaggtcctt gtcctgggcg atggattcgg
    10381 ccacggcgct ggtcagatcc agctccgccg cctggatgcg ccgcgccttg ctgccccagc
    10441 ccagctgcac cagcgagccg ctggtcaggc gccgaaacgg tatgccgcgc gccacggcgg
    10501 cgtcgacgat ggagccggtg gacggcccga tgcggtcgtc ctcgtcgagt tcgcgcagct
    10561 cggcgaccac ggtgtcggcg tcgaagggcg tgtcggccag cgcggcctgc agcagctgtt
    10621 cggcgcgctc catggccagg cggccgaccg attcctcggt gtattcgacg accacctgga
    10681 aggtgccctc ttcgacggtc gcatgcgtgc ggctgaaggt caccgggcaa ccggcctggg
    10741 cctgcagcga cagggctgca gcctccagca catcggccag cgacacgctc tggcccgcgc
    10801 cctggggctg cagggcgccg atctgcggaa agcgcgcgcg cagccggtct tcaaggccgg
    10861 gcagctggct gatgtcgtgc tcctcgggcg cgcattgcac tatggcctcg atggcggtgc
    10921 tgcgggtcca gaggttgggg ccgcgcaggg cgcggatgcg ggtgatttgc atgggtgttc
    10981 tttcagccga attttgattc aaataggtgc ctaacgctta tttgatgcgt cacagtagct
    11041 attatttttg tatcaaatct ggtattcgaa ggtcttgatg ccggcggcga tcagatccgg
    11101 accaatgtgc atggaccagg ccgtggcaat ggctgccagc agggccgtgg tgtccggctg
    11161 ctggccgccg ggcaggctca gcgcctgcag gctgccgagc acgcgctcgc tggcacccgt
    11221 ggccaggatc acctggccgg cgcgcacgaa cacggcgcgg ccctcgccct cggcgcggtg
    11281 cgcggctacg gcggcgttgt cggcgctggt ggagtacagc agcacctcgc cgtcgcacag
    11341 cggcgccaat tcggccacct gagcgatgtc ggcattgagc acgcccgcgc cttcatcgag
    11401 caccacgtcg atctgggtgc gcagcacgcg cgtcatcttg gcctgctcca gcacatcgtg
    11461 gtcggccagg ccctcgtagc cgtccatgtc ggtgaccacg ccgaccaggc agcggtcgta
    11521 ggccaggcct tcttccagga tggagcgggc cgtggtctgt atgaccgccg cctgggccag
    11581 gcggttggtc agcaggcggt gcgcgccggc ccagtgggcg gcattggctt tctgcgtctg
    11641 gcggccggcg atgaacatgc cctcgctgct ggccacgccg gtgagcttgc ccgacagctg
    11701 caacagccag cccaccaggc gcgcgatgaa ggcgttgtgg cgcgtgccga ctatgcccac
    11761 cagcgggatg cggccggcgc ccgggccgtc ctcgcgcggg aacaggtgat cggcaatggc
    11821 catgcccacc gggcgcggcg cgccgctggt cggcttcagg tgcatcagga ggcccgggcc
    11881 ggcgttcacc tccaggatgg cgccctggcc ctgcaggggc ttggagacat ccttgacgat
    11941 catgtccatg ccggcgatgt ccaggccgac gatcttcgcc gccagggtgg cgtagtaggc
    12001 cacgtcgggg tgcacgtcgt cggtgcagtc aatggccatg ttgccgttgc gctgcagcag
    12061 cacgctctgg cccttgggga tgacggtggc gggcgtgacg ttctggcgct tgagttccag
    12121 ctgcaccgcg cccttgtcgg tatcgatcca gtccagcggg tattcctgct cggggccgcg
    12181 gcgcgggtcc tggttcagca cggccaccag ctcggccagc gtggcctggc cattgccgtg
    12241 cacgctcacg gtctcgccct tggtggcggc caccaccttg ccgccgacga ccagcaggcg
    12301 gtgctcgatg ccgtcgatga acttctcgac gatgacatcc gagccctcgg gctgggccag
    12361 cgcgaaggca gccttgatgt cggcctcttg cgacagctcc agcgtcacgc cgcgcgcgtg
    12421 gttgccgtcg gacggcttga cggtgacggg aaagccgatg tcttgcgcca cctcccaggc
    12481 ctcctcggcg ctgttcacga tctggccctc gggcacgggc acgccgcagg aggccagcag
    12541 gcgcttggtg aagtccttgt cctgcgcaat gccctcggcg atggcgctgg tctggtcgct
    12601 ttcggcggtc cagatgcgcc gctgcgccgc gccatagccc agctgcacca ggttgccgtc
    12661 gttcaggcgg atatgcggga tgcggcgctc gccggcggcg tcgacgatgc agccggtgct
    12721 ggggccgaga tagcggtcgt tgatggcggt cttgatggcc tggatggcag ccttgaggtc
    12781 aaacgcctgg tcgttgatgg cggccatcag cagccggtgg ccatgctcga gcgccacgcg
    12841 cgccaccgcc tcctcggggc agcggaacac catgcggtag atgccgcggc gcgatatttc
    12901 gcgcgtctgg ccgaattcgg ccggcatgcc cgagaggttc agcagctcga tgaccacatg
    12961 ctcaagcaca tggcccatcc aggtgccgcc ttccaggcgc tggatgaagc caccgcgctc
    13021 gcccacgccg caggtgtgct ccaccaggtc gggcagccag gccgtcaggc gctcgttcag
    13081 gccgggcagc ttgttggagg gaaagtcctc cagctcgccc agatccagcc agacctcgag
    13141 gatggggcgg taggtccaga cactggggcc gcgcaggaag gtggtgcgca gcagttggat
    13201 atctttgaag ttggccatga agtgcggggt ggaggaatgg ggtggcgcgc cggcgctggc
    13261 gctgcctaag cgtgaattgt gcgtggatat tggcagatgt cagccgccag cggtggagaa
    13321 gcgttacatc gccataccgc tgcgccaccg aggcccggcc cctgctggcc gacaatgccg
    13381 ttcacgggca tggggccctg ccgcagactc cctttgcggg cgcgggcaga catacaaaac
    13441 aaaatgcaac atcaaccatc tgtggacgct tccggtgtct tgcctggccc gctgggggcc
    13501 gaactgcgag ccatgctcgc tcccgacgaa aacgtgcagg ccgccttcga ggttgacctg
    13561 acccccgagc tgcgctttgc ccccggtctc ttggtgctga gcgaccggcg cctgttggcg
    13621 cgcgccatgg acggcgcgac ccagcaatgg ccgctggccc ccggcatggt gctgcgcatg
    13681 ctcgaccatg gcggcgtcgg cacgctggag ctgcatgacg accagcagcg cctggcgctg
    13741 tggcgcttca ccctggggcg ccatccccag gcgctgcacc tgcagcagcg gctggagcag
    13801 cagatcgccc atctgcaaaa cccccaggcc ctggccgttc agcgtgacga ggcgccctgc
    13861 tgcgccgtct gtggcacgcc gctgccggcg ggcagcgacg aatgcccggc ctgcgcgcgc
    13921 cagcaggcca agccgacctc cacctgggtg ctgctgcgcc tgtggcgctt tgcccggccc
    13981 tacaagaagc agctggccgc cggctttggc ctgacgctgg cgtccaccgc cgcctcgctg
    14041 gtgccgccgt acatgaccat cccgctgatg gacgacatcc tcatccccta ccagagcggc
    14101 aaggccatag acccctggct ggtggtgatg tacctgggcg gcctgctgct ggcggcgctg
    14161 ctggcctggg gtttagggtg ggcgcgcact tacattttgg cgctggtgag cgaacgcata
    14221 ggcgcggatc tgcgcaccac cacctacgag cacctgctgc gcctgtcgct ggactacttt
    14281 ggcagcaagc gcaccggcga cctgatggcg cgcataggct cggagaccga ccgcatcaac
    14341 gtctttctgt cgctgaacgc gctggatttc gccaccgacg tgctgatgat cgccatgacc
    14401 tcggccatcc tgttctccat caacccctgg ctggccctgg tcacgctggt gcctctgccc
    14461 ttcatcgcct ggctgatcca caacgtgcgc gaccgcctgc gcaccggctt cgagaagata
    14521 gaccgggtct ggagcgaggt taccaacgtg ctggccgaca ccattcccgg catccgcgtg
    14581 gtcaaggcct tcgcccagga gagccgcgag gcgcagcgtt ttcgcgccgc caaccagcac
    14641 aacctggagg tcaacgacaa gctgaacaag acctggagcc tgttcgcccc cacggtgtcg
    14701 ctgatgacgg aggtcggcct gctggtggtc tggggctttg gcgtctggct ggtggcgcac
    14761 aaccagatca ccgtcggtgt gctggcggcc ttcattgcct acatcgggcg cttttacacg
    14821 cggctcgact ccatgagccg catcgtctcg gtgacgcaaa aggccgccgc cggcgccaag
    14881 cgcatcttcg acatcctgga ccatgtgagc aacgtgcccg atccgcagaa cccggtgcgc
    14941 gtggacaagg tggccggcgc gatcgagatg cagggcgtgg gttttcgcta cggcagccgc
    15001 acggtgatca agaacctgtc gctgcagatt cgccccggcg agatgatcgg cctggtgggc
    15061 cacagcggct cgggcaagag cacgctggtg aacctgatca gccgcttcta cgacgtgagc
    15121 gacggcgcca tcctcgtcga tggcgtggac atacgccgca tggcggtgag cgacttccgc
    15181 cgccacattg gcctggtgct gcaggagccg ttcctgttct tcggcaccat cgccgagaac
    15241 atcgcctacg gcaagcccga tgccacgcgc gccgagatcg tcgccgccgc gcgtgccgcc
    15301 catgcacatg aattcatcct gcgcctgccg cacggctacg acagcctggt gggcgagcgc
    15361 ggccagggcc tgtcgggcgg cgagcgccag cgcatcagca tcgcccgcgc cctgctgatc
    15421 gacccgcgca tcctgatcct ggacgaggcc acgtccgccg tggacaccga gaccgagaag
    15481 gaaatccaga aggcgctgga caacctggtg cagggccgca ccaccatcgc catcgcccac
    15541 cgcctgtcca cgctgcgcaa ggcggaccgg ctggtggtca tggatagagg cgaggtggtc
    15601 gaggtgggcc cgcacgacga gctgatggcc cagcagggcg cctactggcg cctgtacgag
    15661 gcccaggcgc gccgcgccga ggaagacgcc gaggccgccg gcgtgcgcat agaaagccag
    15721 cgccagcaca gcgcccaccc ggccggcaag tgagaggcac agccatgaat accaccacag
    15781 aacccatggc gttcgatctg cgccgcaacc cccatggccg cctggtgctg cgcctggccg
    15841 acggcaccga gcacgaggcc gtgacacccg tgcgcgcctt tccgattgcc gcgccgcagg
    15901 agggcctgtc cctggtcggc gccgacggcc acgagctggc ctgggtgccg cgccttgccg
    15961 atctggcagc tgccgcacgt acgctgatcg aggaagaact cgccgcacgc gagtttgtgc
    16021 ccaccatcac gcgcatccgc agcgtgtcca gcttttccac ccccagcacc tgggaggtgg
    16081 acaccgaccg aggcgccgcg cagctggtgc tcaagggcga ggaggacatc aggcgccttt
    16141 ccgggcgcac caagctgctc atcgccgcca gcgacgggtt gcactttcat gtgcccgaca
    16201 gccaggcgct cgatcgcgcc tcgcgcaagc tgctggagcg atttttgtaa tcatattgat
    16261 agcgctttgc gcttgtctgg cgggcgctgc atggcaatcg gtccaaaagg ccttaatttc
    16321 cccggcagtt tgccgaaatg ggtgtacgac gaggtggtag ctgcggttgc cgccaattgc
    16381 gtattcatga ggagcccatg ccatgcaact gccacccgta gaccgatcgc ccgcccagtg
    16441 gcgtccccag ggcgcggatc tgtattccac gggcgcctcc ggagccgtgc ccgtgcggcc
    16501 cgtgaacgcc gccaatcccg tcgaatccac agaccggctg ggcgagggca gcgtcctgcg
    16561 tgaacccacc aagcccagcg ccccggacga ggaaaaccgc gactggaccc tgccgataga
    16621 gaaaaaagac aagccttcgg aggccgatgc accccctaag gatccaccgc tgcaccaggt
    16681 gatgctggag ttcatccagt ccatgtggcg cgccagcgcc agcgccgtgg atgtggcgca
    16741 ggacatccac aagaccacgc tgcaggagct ccaggcgcag aaggcggagg acgagcagca
    16801 gtcgcttgtc tattccgacc ccaaggtcaa gcgcaccggg ggattgtgag tcttaagcta
    16861 taaattaaat agctgtatgc gcccactgga tgggcgctac cggctggaaa ctacttggca
    16921 atctctgccg gtgcattcgc aatcccgctg ctcacatgcc cggccggcac atggcgcgcg
    16981 gccgagttca catggccggt ctggtcgtcg aagaagaagt cgggttcgaa ctcgcgcaaa
    17041 aactccccct tgggtaggcc gcccagaaac atcgcctcgt ccacggcgat gttccagtcc
    17101 atcagcgtgc gtatggcgcg ctcgtgcgcc ggcgcgctgc gggcggtgac cagggccgtg
    17161 cgtatgcgca tggccgcgcc gccggcctgc tgcagccggt gcagcgcggc cagcaggggc
    17221 ttgaacggcc cctcgggcag gggttgggcg gccttggcgc tctcgtggcg ctggaaggcc
    17281 gccaggccct cgctctggta gacgcgctcg gcctcgtcgg agaacagcac ggcgtcgccg
    17341 tcaaacgcga tgcgcacctc gcgcggatgg gcgtcgccgg cctgcaccga ctcggtcagc
    17401 acgcgcgccg ccggaaaacc caggtgcagg gcggagcgca catcggtctc gttggccgac
    17461 aaaaacaggt gcgcgcccag cggccgcaaa tagcggaacg ggtcgcgccc ctgggtgaac
    17521 acgccgcgct gcacgctggg caggccgtgc gcctggcaag agcggaacac gcgcatgccg
    17581 ctcaccgggt cgttgcgcga cagcagcacc acctccacgc gctggtgctc ggcatggttg
    17641 aaggccagca gcttgcgcac cagcgaaaac gccacgccgg gtggcgcggg gatgtccagg
    17701 cgctggcgct gcagctccac ataggcgcgg tcgtcgccgt gttcgaagac gcggttttcc
    17761 tcctcgaagt cgaacagtgc gcgcgatgag atggcgacga cgagcttgtc gctgagagag
    17821 ggggccatgt cagtcatcgg ggcagaagct taaaaaccgg cgcaacccag gcgcgggcag
    17881 ccgagcaagg gccgccccgc agcgagggct gcgtccccct gcccgcatgg cgtagccatg
    17941 cgagagcggg gggaagcggc gcagccgctc aggggggtgt tgcttcactt cacgaactgg
    18001 ttgagttcga tgatgggctg cagcaccgcg agcacgatga gcatcacgat caggcccatg
    18061 gtgacgatga gcagcggctc caggatcgtc gccagctgca tggcgcggcg ctgcacctcg
    18121 gtgcccagct gggtggcggc gcgctggagc atggtgggca gctggccggt ctgctcgccc
    18181 aggcgcgcga acatggccac caggccgggg aagcgtttct tctgcgccag cgccgaggcc
    18241 agcggcgcgc cctcgcgcac cagcaccagg gcctcctggg cgtcggcgcg cagggcgcgg
    18301 ttgttcagcg tctcggcggc ggcgtgcagg gcgcgcaata tcggcacgcc cgccccggtg
    18361 agcatggcca gggtgccggc aaagcgcgcc gcgttgtagc cgcgcgccag tttgcccacc
    18421 agtggcagct ggagccaggc ggcatcaaac ttttgacaaa aaacgggcct ggccagcgct
    18481 gcacgtgcgc caacagctat caaaacaata aagcccagca tggcccaacc atagccgcgc
    18541 acaaagtcgc tgatggccag catgatgacg gtgagcagcg gcagcgcacg cttggtgccg
    18601 gcaaacacgc tggccacctg cggcaccaca tagcccacca ggaacagcac gatgacgatg
    18661 gccaccaggg tgacgatggc cgggtacagg gccgcgccga tcagcttggc gcgcagctgc
    18721 tgtctggctt ccagatcgtc ggccaggctg tcgagcacct ggctcaagcc cccgctggac
    18781 tcgcccgcgc cgatcaccgc gcagtaaatg tcggagaact cgcgcgggtg ctgggccagg
    18841 gcgcgggcga aggtgctgcc ggcgttgacc tcagcgcgca gcgcggccac cagatggcgc
    18901 tggcgttctt cgtcggcctc gtcggccagg gcggtgaggg cgcgctccag cggcaggccc
    18961 gagcccacca ggccggcgag ctggcgcgtc cagatggata gctgcgtggc gttgaacacc
    19021 ggccgcgtga acaggcgctg cgccaggccg ggcgcgctgc cggtggcggc ctcgggtgac
    19081 agtgcctcca cgtccagcgg caccagcgcc tgggcgcgca gctggttgcg cgccgccttg
    19141 gggctgtcgg catccagcgt gccgcggcgt acatgaccct gggcgtccag ggcttcgaag
    19201 gaataggcgg gcatggcgtc ggcggcgttg aaggcggtca gcctgcgatg gtagcctggg
    19261 ccgtgagggt gagcgagcgc aggcgcagcg cggggtcgaa cacatcgctc accagcatga
    19321 attcatcggc ccgcgtgtcg cgcgccagtg cttgcaggcc ggcgcgcacc gtgtccggac
    19381 cgccgaccac gcccatggcc agaaagtcgc caatggccgc gcgctcgggc gccggcaggc
    19441 cttgcagaaa gccctccacg ggcgggcgca gcaggccgcg ctggccggtg aggatgccca
    19501 gcacgcgctg gtaggtgctg ctcgccagat actcggcctc ttcgtccgtg ggcgcggcaa
    19561 tcaccggcac gccgatcatc acgtagggct tgtgcagcac tgccgagggg cggaacagct
    19621 cgcggtacag ctgcagcgcc ggctgcagca ggcgcggcgc aaagtgcgag gcgaacgcat
    19681 agggcaggcc catgtgcgcc gccagctggg ccgagaacag gctggagccc agcagccaca
    19741 gtggcacctg ggtgccggcg ccgggcgtgg cgatgaggcg ctggcccggc tgggcgggcg
    19801 ccagcagctg ctgcagctcg gccacgtcgc gcggaaagtc ggcctcggtc tccacgcggt
    19861 cgcggcgcag ggcgcgcatg gtggcgccgt cagtgccggg ggcccggccc agccctaagt
    19921 cgatgcggcc ggggtagagc tcggccagcg tgccaaaggc ctcggcgacc accagcggcg
    19981 catgattggg cagcatcacg ccgcccgagc ccacgcggat gtgcgacgtg ctgccggcga
    20041 tatggcccac cagcacggcc gtggccgagc tggcgatgcc cggcatgttg tggtgctcgg
    20101 ccagccagta gcgcttgaag ccgagcgact ccacatgctg cgcggtgcgc acggcaatgg
    20161 ccagcgcatc gccgacgctg ccgccttcgc gcacggcgac cagatcgagc atggaaagta
    20221 cgggggtgtg gttcgtcgtc atggcgctga ttgtgggcgc aagcagtagc ccggcgcggc
    20281 cgtgcggaaa tgtttgttga cagtcaccag gccttgttta gaaaaatgga atcaataatt
    20341 catcagttat caaatataat tttttgtaac gttatgaaac ctgtccgagg ttgcgagtct
    20401 cggatgggca agaactcagg gtgggggtat atgggatttt tcagccgctt gttccagccg
    20461 cgacccgatg gcctgcagac ccattcggac tggtcccgcc tgccggatac cgatgccagc
    20521 gagctggtgc tgttcgacga cgaggccagc aagctcatgg cgcagttcga tatcgacgcc
    20581 gccatcgtca cgcacgagcg ctggctgccc tggctgggcg aggtgctgca gggcgcgcgt
    20641 gacgagcgcc tgcgccccga ggtggtggcc gatgatggct gttccgagct gggccaatgg
    20701 ctgcacggca gcggccgaac ggcgctgggg cattacccgg cgttcgacat gctgctgcgg
    20761 cgccaccgct atttccacca gcaggcggcg gccctgatca cccacgccga ggccggcgac
    20821 atcctgatgg cccagcaggc gcacaaggcc tgccagcatg catcgcgcca ggtggtgctg
    20881 ctgctcaagg agctgcaaaa gggcctgctg cgcacgcgcc ggccggtgct tggtcgataa
    20941 aatttggtgt tttcaggttg cagagcttgt ttggtaagcg gttgacgcta tcttattaat
    21001 agcgtttttt gttgccaatc tgctcaacgc ccgacggtga ccggcaccac gtcgccattg
    21061 gcgcgcttgg cgtaaatgtc ttgccccgca agacggatat cgttgcaggc cgagcggaag
    21121 accttgaact caaagcagac cctgccgtct tcggcgagcc aggtgcccgt gtcggagcgg
    21181 ccggcggcgt agatcgtcag gtggttccct tcggcggcat agtctgcccg gacagtgctg
    21241 ccgtcgggca tgggggttct ggtggatttg ccgcgcagca aagcggccag ctcggccgct
    21301 gatggcgtcc gcacaccggc gggaaaatcc ctggccggtg gcgcggtggc ggcgcaggct
    21361 gtcagcagca gcggcagcag tggagcggac aggcgggtga tggtgttcat gtgagcctcc
    21421 gtgaatcaat cgaatgcgca gcaggcgggc ggctgcccgc ctcacgctgc gacatcacag
    21481 gcccagctgc accttcatcg ccgcccaggc cgtttcgttc aggcggttgg tggtggactg
    21541 gcgcgcgctc aggctgggct tgacctcgtg cgcgcgatag acccagcggc tgctggggtc
    21601 gccggcagtc cattggatct ccgcgtaggc aacgtggtag ttgagcgccg gggtcggcgg
    21661 ttggccggca aagcgcagca cgcgcgcgcc gttgatcgtg tcgatgctgt agctgccctt
    21721 ggcggcgatg gctgcgcagt tggagaccac ggtctgcgct tcgttcaggt cacattcgta
    21781 gtacgtggcc gtgccctggg tggcgtcggt gggggcaaac gacacgcgca ggttcttcag
    21841 tgcgctgctg gtcagcgcca ggttcaaggt gttggcgccg gtgggcgatg ccgcgcccga
    21901 ggttgggtaa tacgcgatca ccgcctccag cgtgttgcgg ctctgcggca gcctacggct
    21961 ccacacgctg gtgatcgtga tgctgggggc gagctctaca ttggtgcgta tccattcctc
    22021 ggcgcctgcg gggaatgcgg cggcgcccag cttgcctgtc aacgccgccg tcgggatgcc
    22081 ggcattgatg gtgttggacg gcatggcctg ccagcggttc accagcgtgg ccatggcctc
    22141 gccggccacg ctgtttgcgc gatcccagcc cagtgtggtt tggccatcgc agtacaccgc
    22201 gcggttcggg ttgcccagcg tgctgcgcag gagcacgcgg cggtcgcagt acttccagcc
    22261 gctcggggta aggaaggcgt tgccatagag cgaatcctcg gtcatgtcca cgccgccgct
    22321 cttgccgctg cgcgcatcga ccgactgctc ctctgcggta cctgctgccc tggcctgccg
    22381 gtcataggcg cgcaggtagt agtcgcccga tccgctgtag ttcagttggc gcaagatggt
    22441 cgatgctggt gcggccgcct gctggttgcc cacctcgagg gcaatgccct ggcgcagcat
    22501 gcccgagacc acggccgccg ccgtgcgggc ggtatcgcgc acctgcgcgt cgtcccaggc
    22561 agggtcgtcc tggtagttgt cgatcttggc ggcaccgatc gccagttgca gcgccacgtt
    22621 ggcgcgcgca tccgcttcgg tcatgccggc ggccacgcct gcctgtgcca gggtggtcag
    22681 cgggttgatg gcgccgcccg agccggccgg gcgcttgagc acgtagtcgg cgtcggtggc
    22741 ggccacggcc gggttgtggg agtcgatggc ggtcgtggcc gcggccgggt caccggtctt
    22801 gacgggggct atcaggcgcg ccgccgcagc ctgcgaggcc gtggtggaca ggctgtactt
    22861 gccatcggcc ccggtggcgc tggatgcggg ttcgccgctg tcgcaggcgt cgttgccgtt
    22921 caggtccagg cagacgatga cgttcttgag cgtgccgagg cgggccactt cgccgctgag
    22981 ggtgatgctg gcgccgctgc cgccgcctcc accgtcgccg ccgccgccgt cggtgtcatc
    23041 gctgcttgag ccgccgcatg ccgccatcgc cagcgcagcg atgatggtga gggagtaggg
    23101 tagccgtctg ccattggtgt atcgcatggt gcgcctcgtt gcttcttggg ttcaggtggt
    23161 tcgtcttgcg acaccgagta cgctaaagga ggcgcttcct ggatacgtca cccagttggg
    23221 tgactccatc tcaaattcca tttcacccgt gtcgttgtgg cgcgccttgc cgtgctgccg
    23281 cgttcactga acatctggcg cctggatgcg aagaacctgg acgcgcaatc actggtcgtc
    23341 gcgccccaac accagcccca tgcggctggc tgccacgatg gcctgcgtgc gcgtggtgac
    23401 gcagagggcg cgcagcacgg cgctgacatg ggtcttggcg gtgttgtggc tcaggcccag
    23461 ctcgcgggcg atcagcttca tcggcttgcc ttccaggatc agccgcagca cctgcgtctg
    23521 gcgtggggta agccccagct cggcagggct ggtgcgcggc tgcgcaggca cggtcagcgc
    23581 aatgcctgcc aggctgagcg gggggatatg gatgccgtgc gccagcacgg tgcgcagcgc
    23641 ctcgaacatc tgctgggacg atgccgactt ggggatgaag cccatcgcgc cgagctggat
    23701 ggcgcgcagc acggtgacct gatcctcttg tgacgacagc atgaccagcg gcatgccggg
    23761 atgtcgttcg cgtatcgtct ccagcgcctg gaagccgttc gcgccgggca ggatcaggtc
    23821 gagcagaacc aggtcgatgc ccgtgtcatg gtcgagcgcg gccagagcct cccccaggtg
    23881 tgcggcatgc accgtctgca ctgccggatc aagctccttg agcagcagcg ccacgccgtc
    23941 gcgaaacaac gcatgatcat cgaccagcag gatcctcatg gtcgcagtct gcaccgtttt
    24001 gccctgtcgc gcctcccccg gttgggggat cgttcaatgc cgcttcgatg gccgccagca
    24061 gccggtcggt gcctgcgggc ttgtgcaaca gcggcagccc ggcgtcagcc acgcgccgca
    24121 ggtcggcagc gcctatgtcg ccggtgatga tgatcgccgg caccgcgtgg ccgatgcgcg
    24181 cccttatccg ctgtacagcg cccaggccgt catgggagcc gcccagccgg tagtcgcaca
    24241 ggatcaggtc ggggtagcgc aggtgttcgt ccagcagcgc caggcactgc cgggtggatt
    24301 cggcgatgag cacatgcgcc ccccagctgt ggcacagcgc ctgcatggcg cggcgcgtgt
    24361 cagcctcgtc gtcgatcacc gcgatgaagg cgccggcgat gcgccgcccg ctggccaggg
    24421 ccggtggcag cggcgccgcc accggcagcg cctcggccgc cggcagcagg atggaaaaac
    24481 ggctgccccg gcccggcgcc gagcgcagcg tgtgcggatg gcccagcagg tcgagcgtgc
    24541 gcttgacgat ggccagcccc aggcccagcc ccttgctgcg gtcacgctcc gggttgccca
    24601 cctggaagaa ctcgtcgaag acatgcggca gctgagccgg cgcgatgccg atgccggtgt
    24661 cccatacctg cagctcgatg gcgccgggcc ggcgccggca cccgaccagc accctgccgc
    24721 gctcggtgta gcggatggcg ttggacacca ggttggccag cacccgctcc agcagcacgg
    24781 cgtcggtgcg cacggcgcag cgcgaaggca ccaccgtcag ccgaagccgt ttgtgctcgg
    24841 cctcgggcgc gtggctcgcc gccacgcggt gcagcagctc ggccagggca tggtcgccca
    24901 gcacggggcg ggacatgccg gcatcgagcc gggagatgtc gagcaggcca acgaacaacg
    24961 cctccatggc cgagatggaa gactggatgt ggcccaccag ctggccgacc tgcggcgtga
    25021 cctcctgcct gcgcagcacc cccaccagca ggctgatggt gtgcagcggc tggcgcaggt
    25081 cgtggctggc cgatgccagg aagcgcgtct tggcctggct ggcctgctcg gcctcctcgc
    25141 gccgggcctg cagcagggcg ttggccttgg ccaggtcttg cgtgcgttcg gccacgcggg
    25201 tttccagcag ccgcaacgag tcgcccaggc gctcggccat ctggttgaag cggtcggcaa
    25261 gctggcccag ctcgtcgctg cgcgccagct cgatgcgatg gtccagctgg ccttcgccga
    25321 tgcggtctgc gccttcggcg agttgctgga tcggccgtac cacccggcgc gccagcacga
    25381 tgccgacgcc cgatcccgtc agcacggcaa gcaccaggat cagcagcgcg gtggccagcg
    25441 cacggcgtac cggcgcgtat gcttcggcgc gcggctgctc ggtgatcagc cgccagccgg
    25501 gtacgctcaa ctcggcgacg cgggcaacca tcgggccctc aggcgtctcg aattcgatca
    25561 gtccgccgcg gccagccgtg cgcaccggtg ggcgcgtggc ccagtcagtg agcgtcagta
    25621 cccgcaagaa atccgggtgc gccagcagcc gcccttggcc atcgacgacg taggccacac
    25681 cgtcgctgcc gaagcgggtt tcatggatga tgtcacgcaa ttccgacagt tcgacctccg
    25741 ccgcgagcac cccgctttct ggcagccggc cgcgccgggc gatcaccacg cgcggcaggt
    25801 tgacggcaaa cgtgaccggg ccgaaccatg gcgcatcggc gcgggcgtgc aggaactcgg
    25861 gagactggct gcggtcttca cccccgccga tgcggtcggg cgccatgcgc gagatacgca
    25921 cgcgttcctg gccgtgcggg tcgatccagt acagcgtctg gatcgcccgt tctgccttca
    25981 tcaaccggta cagctcgtac tggatgtcgg cgtcggcgtt gcccagatgg cgtgaatcct
    26041 gcagcgacag ggtggtgcgc agcagggcag ccgtctggct gatgaagcgc tcaatcttgg
    26101 ccgaggcctt ggcggcttcg agcgcctgta ctgcctgcac cgcaacgcgc gtctcgcgct
    26161 ggatcagcaa gagctgcgcc aggccgctgg ccgtgagtgg cagcgccacc agtgcgacca
    26221 ccgtcagcgc ataggtctgc cacaggcggg ggccggacgg gcgcatcagc gggcgcgcgc
    26281 gggaggctgg aagggaggca ggggcgtggc gtcggctctc ggcgccagcg tgcgctcgcc
    26341 gggctggatc aggcggtcgg cgcgcgcgat cagcgcgggc ggcagcgtta cacccagacg
    26401 ctgggcggcg tcgaggttga cgaccagcgc cgcttcgtac agttcttcga tcggcagctc
    26461 ggccagcggg cggccgcgca gcacttggtc gatgtagcgc gccccgcgcc cgcacatgtt
    26521 gacgcgcccg acggcgccgt agctgagcag gccgccataa cgcgtggcga accaggagtt
    26581 gtgtgcgatc gccggcatgc ccacgcgctg cgcctggcgc gcgtgctcgc cgagaaacgc
    26641 cgaggtatcc ggccggatca tcacggcatc gaccttgcgt ccggccagct cgtcccaggc
    26701 gctggcaatg ccggcctcgc ccgagaattt cacgcgcacc aactcggcgc cgatctgcct
    26761 caccatgtcc gtgatcggcc cgaccgatcc cccgtcgttg tgtcctggtt ggtcgttgtt
    26821 ttccatcatg ccaatgcggc gggctgtcgg aaacgcctgc agcaacacct ccaccggctt
    26881 gatcgcatac tggttgttca ggcccagcga gacgccggtg acattggcca acgggcgcaa
    26941 ttgcgcatcg aggacatggt gcaggttggt tggacccatg acgacgatcg gcagcttctg
    27001 cgtggccttc atcagggcct cgttcggcat gttctcggtg gcgatcagga tatcgggccg
    27061 gctggccacg atcgcctggg ccatcggggt gagctgggct ggatcgttgc ctgcgtcgtt
    27121 ccattccagc gccagttgac cgtcgaatga ccagccggac tcctgcaggg cgcggcgcac
    27181 ctggttcatg cagacccgca ccgactcgct cggcccggtg cccaggtagg cgatacgccg
    27241 gggcgcggcg gtttgcgcgc cggcgggtgg gctggtggcc aataaggcgg cgagcgcggc
    27301 aatgaggatg ggccgaaggc cgcacggaat cgagggcatt cgccagcgta gcggcgccgc
    27361 cgccgcgctg tcaatcgcgc gttacgcgca gcacttcctc gcggctggtg atgccggcgg
    27421 cgaccagtcg ctcgccgtcc tcgcgcatca gctgcatgcc gccggccagg gcctgggcgc
    27481 gcacctcggc ctcggccg
//
LOCUS       DAOMTQ010000019        27289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_162_length_27289_cov_26.740471, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000019 DAOMTQ010000000
VERSION     DAOMTQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 27289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27289
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_27289_cov_26.740471"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..1022)
                     /gene="mobA"
                     /locus_tag="PK518_03450"
     CDS             complement(387..1022)
                     /gene="mobA"
                     /locus_tag="PK518_03450"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HRM47995.1"
                     /translation="MIDTCDITGLILAGGRGSRMGGVDKGLQGFRGQPLALHALRRLQ
                     PQVGSVMISANRNLPAYEAFGVPVWPDGLADHAGPLAGFLIGLEHCTTPWLLTVPCDS
                     PRFPADLAGRLAAAATAQGADIAMAAAPEQQHDGSTRLRTQPVFCLLRVNLLQSLQGF
                     TADGGREIHRWAAMHPCAVMPFDQAGDDPLAFFNANTLAQLHALDSAQTPC"
     gene            complement(1032..2174)
                     /gene="moaA"
                     /locus_tag="PK518_03455"
     CDS             complement(1032..2174)
                     /gene="moaA"
                     /locus_tag="PK518_03455"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRM47996.1"
                     /translation="MAERVIPLVDQRSAALRPAVPSAAQAAPATGQLHDRLSRPLRDL
                     RISVTDRCNFRCGYCMPKEVFDKHYRYLPHGDLLSFEEITRLARLFMQHGIHKIRLTG
                     GEPLLRKDLEVLVAQLAGLRTTEGRVPDLTLTTNGSLLARKARALKAAGLQRVTISLD
                     SLQDEVFRRMNDVDFPVAEVLAGIEAAQAAGLAHIKVNMVVKRGTNDQEIPAMARHFR
                     GSGITLRFIEYMDVGATNGWRMDQVVPSSEVIARLQSELPLVPLAATAQGETAKRWGW
                     AGADGRHDPALGEVGVISSVTQAFCGDCNRARLSMEGRMYLCLFATQGWDLRSLLRSD
                     ASDAQISTAIAHIWQGRGDRYSELRASLPADSGAGQRRIEMSYIGG"
     gene            2441..3154
                     /locus_tag="PK518_03460"
     CDS             2441..3154
                     /locus_tag="PK518_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HRM47997.1"
                     /translation="MALATAINRTARFRAMSQRTAKLYGQLHLNVRPERAREALEQTR
                     RQVRADLDELDRQAWPPEVARLLADVRRSAERLDGLTAQPAGKSGLQAVSAQADQMLT
                     HANSATQALEHLAQAPTARLVNMAGRQRMLSQRLAKNYGLVAAGLDSRPLREQIAGDA
                     EEFQRAMAQLAGAPLSTPAIRTTLELGQGQWVFFDMAIKRQPNARGLEDVATTSERLL
                     EVMDSLTALYEVALQDVLG"
     gene            complement(3220..3777)
                     /locus_tag="PK518_03465"
     CDS             complement(3220..3777)
                     /locus_tag="PK518_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HRM47998.1"
                     /translation="MPLSPPSPRTARHIRRVNYQGFERDDGLWDIEGELHDSKTYDLP
                     SLRHPGQLRPAGDPIHHMWLRVTVDGDLVVRAIESAMDAHPLAGCPEAQRALQSMVGA
                     SMVRGWRQSIQTHLGGVAGCTHLRELLFNLATATFQTINAAFKSRGDEPPRHLGQCTG
                     WDYHGEGVRQHYPRFYQQPKKLQNK"
     gene            complement(3807..5240)
                     /locus_tag="PK518_03470"
     CDS             complement(3807..5240)
                     /locus_tag="PK518_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRM47999.1"
                     /translation="MQYHYIANAAVASSAGRTIAVIDPSDGQPYDEIQRGTGEDIDAA
                     VRAAQQCYDGVWRSLAPAERGRLLQRLSAKITENADELAAIEQRDCGKPTRQARADAA
                     ALARYFEFYAGACDKLHGETIPYQEGFSVLTWREPHGVTGHIVPWNYPMQIFGRCVAA
                     ALAAGNVCVVKPSEDACLSLLRVAQLAAEAGFPAGAINIVTGYGHEVGDALARHPGVR
                     HISFTGSPRIGTLIQQAAAERHCPVTLELGGKSPQIVFADADLDAAVPVIVNAIVQNS
                     GQTCSAGSRLLIDSLIYEPLLERLAQAFESLRVGPAAMDLDLGPLIRQSQQQRVWDFL
                     SDAQVAGIPLVAQGTVVDEAPDTGFYQAPTLLRDVPADHRLAQQEVFGPVLAAMPFHD
                     EDHAVELANATPFGLVAGVWTRDGARQLRMARRVASGQVFINNYGAGGGVELPFGGVK
                     SSGHGREKGFEALYGFTTLKTVAIRHG"
     gene            5571..7670
                     /locus_tag="PK518_03475"
     CDS             5571..7670
                     /locus_tag="PK518_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRM48000.1"
                     /translation="MTPNPSTHGSPLRSFLIWGAVAIIGAWALGVIALQRGEPINAVW
                     LIVAAICVYLIGYRFYSKFIAEKVLELDASRMTPAWRHNDGLDYVPTDKYVLFGHHFA
                     AIAGAGPLVGPVLAAQMGYLPGMLWILAGVVFAGAVQDFVVLFISTRRDGKSLGDLIK
                     TEMGPVAGVIALFGAFMIMVIILAVLAMIVVKALIHSPWGVFTVAATVPIALFMGIYL
                     RFIRPGRIGEVSVIGFVLLMLAIVYGQDVAASPTLAPWFDYSGVGLTWILIIYGAVAA
                     MLPVWLLLAPRDYLSTFLKIGFIVLLAGSIVIVAPQMQMPALTRYIDGTGPVWSGSLF
                     PFLFITIACGAVSGFHALISSGTTPKLLENETQARLIGYGGMLMESAVASMAMIAACV
                     IEPGVYFAMNSPAALIGTTPEQVAQVVSSWGFAVTPEVLLATAKDVGETTIISRAGGA
                     PTLAVGMAHILHQLFGGEGMMAFWYHFAILFEALFILTAVDAGTRAGRFMLQDLLGTF
                     VPSMGRTDSLPANVISTCLCVAAWGFFLYQGVVDPLGGINTLWPLFGIANQMLAALAL
                     TLGTVVLFRMKKDRYAWVTVVPTVWLLACTLTAGWMKLFSADPRVGFVATARKFSAEV
                     ARHQAEGTLPEKGPTIEQMQQIVFNNYLDAALCAVFMLVVVSGVIFGIKAVLHARSHN
                     KPSVRETPYQPLPATGA"
     gene            7675..7896
                     /locus_tag="PK518_03480"
     CDS             7675..7896
                     /locus_tag="PK518_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HRM48001.1"
                     /translation="MPGLVTLSSLAHAAQESGSYLARAARMMVGVPDYGQYLAHMRRE
                     HPQEAPLNYEEFFKNRMQARYGGGRAGCC"
     gene            complement(7985..10441)
                     /locus_tag="PK518_03485"
     CDS             complement(7985..10441)
                     /locus_tag="PK518_03485"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRM48002.1"
                     /translation="MAGQAALTSPLILALACGLLAVVYGLWARSWILSKDAGNARMQE
                     IAAAIQAGAAAYLARQYKTIAVVGVVLAVLIGLFLDTLTAAGFVVGAVLSGACGFIGM
                     NVSVRANVRTAQAATQGMGPALDVAFRGGAITGMLVVGLGLLGVTGFAWFLLGRSGAD
                     AQLAATLNPLIGFAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPA
                     VIADNVGDNVGDCAGMAADLFETYAVTLIATMVLGALLIGAAPTAAVAYPLALGAVSI
                     VASIIGCFFVKASPGMKNVMPALYKGLAIAGVLSLVAFWFVTAWIIPDNALGGSGAAI
                     RLFGACATGLVLTAALVWITEFYTGTQYSPVQHIAQASTTGHGTNIIAGLGVSMRSTA
                     WPVLFVCAAIIVSYMLAGLYGVAVAAMSMLSMAGIVVALDAYGPITDNAGGIAEMSEL
                     PSSVRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLDSYGQAVKFDLSD
                     PMVIVGLFIGGLIPYLFGAMAMEAVGRAAGSVVVEVRRQFSEIKGIMEGTAKPEYGRA
                     VDMLTSAAIKEMIIPSLLPVAVPIVVGLALGPKALGGLLMGTIVTGLFVAISMCTGGG
                     AWDNAKKYIEDGHHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIV
                     PLVVAMHGGTKVAATAPAAPTAAVATADDAASVVIENEKVIFYFASGKAEPHADGAGA
                     LARIVDGVKAGKKAVISGYVDSTGSAAANAEIAKQRALAVRDLLMGLGVAEDQVELQK
                     PADIQAGSGAQARRVEVVLM"
     gene            10711..11241
                     /gene="ppa"
                     /locus_tag="PK518_03490"
     CDS             10711..11241
                     /gene="ppa"
                     /locus_tag="PK518_03490"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRM48003.1"
                     /translation="MSLNNVKPGTNIPDSFNVVVEIPAESDPVKYEVDKESGAVFVDR
                     FLTVAMYYPCNYGYIPQTLSGDGDPVDVLIITPYPLLPGVVVPCRALGILKMEDEAGV
                     DGKVLAVPTTKILPMYENWKTVEDVNPMRLKAISHFFEHYKDLEDGKWVKVLGWGGID
                     AAHKEILDGVESYKKG"
     gene            11478..11984
                     /locus_tag="PK518_03495"
     CDS             11478..11984
                     /locus_tag="PK518_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRM48004.1"
                     /translation="MLSATAQRPSTSNAATSNAAEYLTFRLGQEEYGIDILRVQEIRS
                     YEQPTRLAHAPDFIKGVIDLRGVIVPIVDLRLKLHCAQAEYTDFTVVIILNVGGTVLG
                     AVVDAVADVVALQAEAIKPAPQFQGQVDSSFVRGIATVGERMLIVLDIESLLSGSEMG
                     LVQAAAMV"
     gene            complement(11974..13209)
                     /locus_tag="PK518_03500"
     CDS             complement(11974..13209)
                     /locus_tag="PK518_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM48005.1"
                     /translation="MPLTSTTPPYELRLHDAASQVDATAWDALLALQSQPTPFMRHAY
                     LAALETSGSATARTGWVPRYFTLWQGERLCAACALYIKAHSYGEYVFDWAWADAHERH
                     GLPYYPKAVVAVPFTPVPGSRLLAQGDASRTALLRALLDWARESGLSSLHLLFAADDE
                     LATARAAGLLLRHQVQFHWHNRHPQNAARFADFDDFLASLTQDKRKKIRQERRRVLEA
                     GVVFRVLRGREITAADWDFFYHCYQRTYLEHGNPPYLTRGFFTLMQEQMPEAWVLFVA
                     ERGGQRIASSLIAVSAYPSSVRSENSLTSTAYGRYWGALERVDCLHFEACYYQPIAWC
                     IAQGVRRFEGGAQGEHKMARALMPVQAGSAHWLAHPAFAQAVGHFLAREGQGVQHYLE
                     DLDNRSPFRRAHQDGRIKP"
     gene            13310..14974
                     /locus_tag="PK518_03505"
     CDS             13310..14974
                     /locus_tag="PK518_03505"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRM48006.1"
                     /translation="MSFAISIAQLNFVVGDLPGNARKIIRAAQDAHAAGARLLLTPEL
                     ALCGYAAEDLYLRPAFLAACEQALAEVVAATAALPGLAVVVGHPQRLPHGIGEGGRNL
                     CHNAASVLRAGRIEQTYAKQYLPNYEVFDERRYFVPGTDSCVFEVDGLRLGLLICEDA
                     WYPEPARRAREAGAQMLLTINASPYHLGKGGEREQVMRERVQETGLPLVYAHLVGGQD
                     EVVFEGRSFALNADGSVAARAPGFEEKMLFAQVQQAQAAITIEADVAPREGWEAELWA
                     ALVLGVRDYVGKNGFPGVLLGLSGGMDSALVLAIAVDALGADKVRTVMMPSPYTADIS
                     WIDARDMAERLQVRYDEIAIAPQFEAFKAALAPQFAGRAEDTTEENLQARIRGTLLMA
                     LSNKFGSVVLTTGNKSELATGYCTLYGDMAGGFAVIKDVLKTRVFALARWRNAHDPYG
                     TGANPIPERIITRPPSAELRPDQTDQDNLPPYEVLDAIVERYMENDEPVEDIVRAGFA
                     HADVERVTRLIQVNEYKRRQAPVGVRVTRRSFGKDWRYPMTNKFRA"
     gene            15017..15355
                     /locus_tag="PK518_03510"
     CDS             15017..15355
                     /locus_tag="PK518_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRM48007.1"
                     /translation="MQMITAVIKPFKLEEVREALAECGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVRDQDVDRCVDAIVGAARTGKIGDGKIFVTAVERVVRI
                     RTGELDDSAV"
     gene            complement(15366..15959)
                     /locus_tag="PK518_03515"
     CDS             complement(15366..15959)
                     /locus_tag="PK518_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRM48008.1"
                     /translation="MADMAFSVCVYLGSRQGDNPLFTEAAQAVGRWIGRHGGQLVYGG
                     GRSGLMGTVAEATRLAGGRVVGIIPQALVDKELANQLCDELHVVTSMHERKAMMAERS
                     DAFLALPGGIGTLEELFEVWTWRQLGYHDKPVGLLDTDGYYSGLMAFMQHAVKNGLMS
                     EWQMGLIRTGTEPAGLLAALVQDAGLNATTIPLRDVI"
     gene            complement(15959..16348)
                     /locus_tag="PK518_03520"
     CDS             complement(15959..16348)
                     /locus_tag="PK518_03520"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRM48009.1"
                     /translation="MPDTPAPHPHKQRTGLNRLWHATGYSLAGLRAGWREKAFRQEVL
                     LAAVLLPLACWLGRGWVETALLTASVVLVLITELLNSGIEAAIDRIGPELHDLSKRAK
                     DMGSAAVLLSLLLCGAVWAAALFQRFS"
     gene            complement(16366..16860)
                     /locus_tag="PK518_03525"
     CDS             complement(16366..16860)
                     /locus_tag="PK518_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRM48010.1"
                     /translation="MAPPLHRRMACWLYEGMLMFGVVFIAGYLFGTLTQTRNALDNRH
                     GLQAFLFIVFGIYFVWFWSRGHTLAMKTWHIRVVDRQGRLISQKRALLRYMLSWIWFV
                     PPLAVIAPYHLSGGASAVIVLGWVAIWALLSRLHPQRQFWHDALAGTRLVHHELPPAP
                     RKKK"
     gene            complement(16966..17784)
                     /locus_tag="PK518_03530"
     CDS             complement(16966..17784)
                     /locus_tag="PK518_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HRM48011.1"
                     /translation="MHLAPPRTPAHRLPAFVLAFVLLGVLAAGGWWMTAYTRIAPSTP
                     MPAQALSGGKFGEMRFAPQPEVGPPWSTLTTRQKEALYPLANRWSLLSELQKRNWLNL
                     AAGFHALTPEEQEKMLERITDWASLSAQQRSQARLNYAAAAKLPTDDRRAQWEAYQAL
                     SAEEKKRLAARAAPRPRGAATAIHPVSPRKLARVPAATEAPPAVLNPPKIVAPTVLHP
                     APITLPPPPIAVETAPINTPSAVAAPLAPLEPASGASEAKEEPARYYPPLHPPE"
     gene            complement(17813..18274)
                     /locus_tag="PK518_03535"
     CDS             complement(17813..18274)
                     /locus_tag="PK518_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HRM48012.1"
                     /translation="MNRTTEPSMQANQAADAYARRLTARLTHGNADLPYDITERLRAA
                     RMQALAQRKRPAIQTVQQTESATQILVHGASASLGGWGREGGNWWRAVMSAVPLAALV
                     IGLFVVNMEQDDAVASEIAEVDAALLTGDLPPSAYADPGFVQFLKSSHQTP"
     gene            complement(18271..18840)
                     /locus_tag="PK518_03540"
     CDS             complement(18271..18840)
                     /locus_tag="PK518_03540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRM48013.1"
                     /translation="MATEQELSDFLKSVDKRAFKRTLYHVRNEEAALDIVQDSMLKLA
                     EHYGDKPPGELPMLFQRILTNCTLDWFRRQKTRNALFSSLSDFEGPGEDGADFDFLEV
                     HAGVPEGETAQSAEDSLRRAQVLRAIETEIQLLPARQREAFLMRYWEEMDVAETAAAM
                     GCSEGSVKTHCFRAVQTLSKALKAKGIVL"
     gene            19127..20905
                     /locus_tag="PK518_03545"
     CDS             19127..20905
                     /locus_tag="PK518_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HRM48014.1"
                     /translation="MEISKAELTSAAAASQASGSQELMGAEILVKALQAENVQYIWGY
                     PGGAVLHIYDALYKQETMQHVLVRHEQAAVHAADGYARATGDVGVALVTSGPGVTNAV
                     TGIATAYMDSIPMVIITGQVPTPAIGLDAFQECDTVGITRPIVKHNFLVKDVRDLALT
                     MKKAFHIARTGRPGPVVVDIPKDVSFKKAPYNGYPKSVEMRSYNPVKKGHAGQIRKAL
                     QLLLTAKRPYIYTGGGVLLGNATQELRTLVDMLGYPVTNTLMGLGAFPATDRRFVGML
                     GMHGTIEANNTMQNCDVLLAVGARFDDRVIGNPKHFAQNDRKIIHIDIDPSSISKRVK
                     VDIPIVGDVKEVLAELIAMIRETTTRADAGALAAWWDQIESWRGRDCLKYDRGNTEVI
                     KPQYVVETLWNMTRDVEAYITSDVGQHQMWAAQFYKFDEPRRWINSGGLGTMGVGIPY
                     AMGIKLAKPDSEVFCITGEGSVQMNIQELSTCLQYNTPIKIVSLNNRYLGMVRQWQEI
                     EYSGRYSHSYMDALPNFVKLAESYGHVGLLIERPQDVEPALREARKLKDRTVFLDFRT
                     DPSENVFPMVRAGKGITEMLLGSEDL"
     gene            21093..21584
                     /gene="ilvN"
                     /locus_tag="PK518_03550"
     CDS             21093..21584
                     /gene="ilvN"
                     /locus_tag="PK518_03550"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRM48015.1"
                     /translation="MKHIIAVLIENEAGALSRVVALFSARGYNIESLTVAPTEDASLS
                     RMTIQTTGSDDVIEQITKHLNRLIEVVKVVDLTEGAYTERELMMVKVRAVGKEREEMK
                     RMADIFRGRIIDVTDKSYTVELTGDQSKNDAFLQAIDRTAILETVRTGASGIGRGERI
                     LRV"
     gene            21634..22650
                     /gene="ilvC"
                     /locus_tag="PK518_03555"
     CDS             21634..22650
                     /gene="ilvC"
                     /locus_tag="PK518_03555"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRM48016.1"
                     /translation="MKVFYDKDCDLSLIKGKTVAIIGYGSQGHAHAQNLNDSGVKVVV
                     GLRKGGASWDKVGKAGLNVLEVNDAVKAADVVMILLPDEQIAEVYKNNVEPNIKQGAS
                     LAFAHGFNVHYNQVVPRADLDVWMVAPKAPGHTVRNTYTQGGGVPHLVAVHQDKSGKA
                     RDLALSYAMANGGGKAGIIETNFREETETDLFGEQAVLCGGAVELIKMGYETLTEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPEVINEQSRVAMRN
                     ALKRIQDGEYAKMFIQEGRLNYPSMTARRRNLAAHSIEQVGAQLRAMMPWIAKNKLVD
                     QSRN"
     gene            22828..23664
                     /gene="pssA"
                     /locus_tag="PK518_03560"
     CDS             22828..23664
                     /gene="pssA"
                     /locus_tag="PK518_03560"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRM48017.1"
                     /translation="MHDGDEATENTGVLVHKRRKGIYVLPNLFTLAALFGGFYAIVMA
                     MNGRFELASMGVFCAMVLDSLDGRVARMTNTQSAFGEQMDSLSDMVSFGAAPALIAYM
                     WALQGLGRWGWIAAFVYCACAALRLARFNVNTGVVDKRFFQGLPSPAAAALVMGFIWL
                     LTDAGVHPGQDLLGLSWVHITWVMFGFTLYAGLTMVTNVPFYSFKDLHMKKSVPFAAI
                     VLIALGIAVVNIHPPTVLFGIFVLYGLSGYVLYAWRKAKGQHTSVISTSTDEPDEQGL
                     HH"
     gene            complement(23671..24324)
                     /gene="maiA"
                     /locus_tag="PK518_03565"
     CDS             complement(23671..24324)
                     /gene="maiA"
                     /locus_tag="PK518_03565"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRM48018.1"
                     /translation="MPRLYSYFRSSAAYRVRIALALKGLAYETVPVHLVKDGGQQHAS
                     GYRSANPQGLVPALQPAVGGPVLAQSLAIMEYLDEVHPQPALLPADALGRARVRALAQ
                     MVACEIHPLNNLRVLQYLTQELGVSGAQKDAWYAHWVALGLQAVEDSLARSPDTGRFC
                     HGDTPGLADCCLVPQVFNARRFNCALDAYPTITRIVAACEQLPAFQQAAPSAQPDAE"
     gene            complement(24395..25408)
                     /locus_tag="PK518_03570"
     CDS             complement(24395..25408)
                     /locus_tag="PK518_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRM48019.1"
                     /translation="MKLATLKQGGRDGTLVVVSRDLKRCRAVPAIARTMLAAMDDWAT
                     VEAQLRQVYEALNSGAIEGEAFDQNACHSPLPRTWQWADGSAYINHVELVRKARNAEV
                     PESFYTDPLMYQGGSDGFIPPRGKVLAQEAWGIDFEAEVTVVTGDVPQGATPEQAAKA
                     IRLVMLVNDVSLRNLIPAELAKGFGFFQSKPASAFSPVAVTPDELGEAWKDAKVHLPL
                     VVHLNEQLFGKPNAGVDMTFDFGQLVAHVAKTRTLCAGSIVGSGTVSNKQGDLWGSSI
                     ANGGMGYCCLAEVRTYETIEQGKPATPFMKHGDRVRIEMFDGQGQSIFGQIENEVDTH
                     GAA"
     gene            complement(25446..26825)
                     /locus_tag="PK518_03575"
     CDS             complement(25446..26825)
                     /locus_tag="PK518_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM48020.1"
                     /translation="MSHAHSAAAVDIGRTLDHGPFSSLQKMVVLMAALSIILDGFDGQ
                     LIGFAIPVMMKEWGIARSAFAPAVATGLVGMAIGSAFAGYMADRIGRRLVIAASVALF
                     GATTIAIGFAPNIDAITVLRFIAGLGIGGALPSASTLTAEFTPLKYRTVAVTGTIVSY
                     PLGGMLAGLFASFVLPTLGWRAMFWIGGALPLAYSFFLLARLPESPRLMARHSAQWPQ
                     LRTLLARMQRPTAADAPFVDAVEQAAGQKQNAGFGALFKDGLARDTLAIWMGFFMVML
                     ASYSAFSWLPSMLTAEGLPVSMASSGLTAYNLGGVLGALGCAWAMSRFGSRWPLALAC
                     AGAAASALALKGVDFHQDASLLMLGLGVHGMFVNGVQAPMYALCAHVYATHIRATGTA
                     AGLAFGRLGAILSSFAGAAIITSNGAQGYLSLLGYAMLAALVALFLVKHHIRGRRAAD
                     TPSTNAKAA"
     gene            complement(26991..>27289)
                     /locus_tag="PK518_03580"
     CDS             complement(26991..>27289)
                     /locus_tag="PK518_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM48021.1"
                     /translation="QHLALKVDSVETMMKVKAKMEADGIEVIGPTNHTIFQSIYFRDP
                     SGHRLELAANTSTPQMDKALDDVKWDMLNEWDRTKQAPTHARWMHDGSGRVGPH"
BASE COUNT         4625 a         8589 c         9274 g         4801 t
ORIGIN      
        1 cctcgcgttg aggggcttgc ggcaggtgtt gggcgctgaa gggcattcca gttcaatcca
       61 tcaaacatcg atcgtggtgc cgacgatatc acctgggatc gccatggtgg gggcattgca
      121 tataaaacta ttgtttttat agctgtcggc gcttatctat aaagcgcgac atgccaattt
      181 tgcctgacta tgcacatatc gcagaggccc ctcacccctc cctctcccca aaggggcgag
      241 ggcgaccggt tgcgccggct ttactccctc tccctctggg agagggttgg ggtgagggct
      301 cgcggcacag tctgaggtat atgcctaatc aggcaatttt gttgagattt catgcggctg
      361 caagcccatg gcctggcaag gccgggtcag catggcgtct gcgcgctgtc gagtgcatgc
      421 agctgcgcca gggtgttggc gttgaagaag gccagcgggt cgtctccggc ctggtcaaag
      481 ggcataacgg cgcaggggtg cattgctgcc cagcggtgga tctcgcgccc gccatcggcg
      541 gtaaagcctt gcaggctctg cagcaggttc acgcgcagca ggcaaaacac aggctgggtg
      601 cgcagccgcg tgctgccatc gtgctgttgc tcgggggctg cggccatggc gatatcggcg
      661 ccctgggcgg tggctgcggc cgccaggcgg ccggccaggt cggccggaaa gcgtggactg
      721 tcgcagggca cggtcagcag ccacggggtg gtgcaatgct ccagcccgat caaaaagccc
      781 gccagcgggc cggcatggtc ggccaggccg tcgggccaca ctggtacgcc aaaggcctca
      841 taggcgggca ggttgcggtt ggcgctgatc atcacgctgc caacctgggg ctgcagtcgc
      901 cgtagcgcat gcagcgccag gggctggccg cgaaagccct gcaggccctt gtccacgccg
      961 cccatgcgcg agccgcgccc gccggcgagg atcaggcccg tgatatcgca cgtgtcgatc
     1021 atgctccgcg ctcagccgcc tatgtagctc atctcgatgc ggcgctggcc tgcgccgctg
     1081 tccgctggca ggctggcgcg cagctctgaa tagcggtcgc cgcggccctg ccagatatgg
     1141 gcgatggcgg tgctgatctg cgcgtcgctg gcatcgctgc gcagcaggct gcgcagatcc
     1201 cagccctggg tggcaaacag gcacaggtac atccgcccct ccatggacag gcgggcgcgg
     1261 ttgcagtcgc cgcagaacgc ctgggtgacg ctgctgatca cgcccacctc gcccaaagcc
     1321 gggtcgtggc ggccgtcggc gccggcccag ccccagcgtt tggcggtttc gccctgggcc
     1381 gtggcggcca gcggcaccag cggcagttcg gactgcaggc gcgcaatgac ttcggatgag
     1441 ggtacgacct gatccatgcg ccagccattg gtggcaccca cgtccatgta ttcgatgaag
     1501 cgcagcgtga tgccgctgcc gcggaaatgc cgcgccatgg ccgggatctc ctggtcgttg
     1561 gtgccgcgct tgaccaccat gttgaccttg atgtgggcga ggccggcggc ctgcgccgcc
     1621 tcgatgccgg ccagaacctc ggccacggga aagtccacgt cgttcatgcg ccggaacacc
     1681 tcatcctgca ggctgtccag gctgatggtg acgcgctgca gcccggcagc cttgagcgcg
     1741 cgcgccttgc gcgccagcag cgagccgttg gtggtcagcg tgaggtcggg cacccggccc
     1801 tcggtggtgc gcagcccggc cagctgggcg acgagcacct ccagatcctt gcgcagcaag
     1861 ggctcgccgc ccgtgaggcg gatcttgtgt atgccatgct gcatgaacag ccgcgccagc
     1921 cgcgtaatct cttcaaagct gagcaggtcg ccatgcggca ggtaacggta atgcttgtcg
     1981 aacacttcct tgggcatgca gtagccgcag cggaagttgc agcgatccgt gacgctgatg
     2041 cgcaggtcgc gcagcggccg gctcaggcgg tcgtgcagct gaccggtggc gggtgccgcc
     2101 tgtgctgcgc tgggcaccgc gggccgcagc gccgcgctgc gctgatccac gaggggaatg
     2161 acacgttcag ccatgggcaa attgtgcccc atggggcgag taggggcttg gctggtgctg
     2221 cgccgattga taccggtcaa tggatcgctg acctggttca cggttgatgt gggtcaattg
     2281 cggggcggcg cggcatttgg acaatggcgc catgcttaac atttgtttac cctgtgtttc
     2341 tctcggcatt acccaggttc atcgtggccg ccgtgtcgcg ttgcgcgcgt tggctgccgc
     2401 gatgctgatg ccggcgttgc catcgaccgc cctggcgcgc atggctttgg ccacggcaat
     2461 caaccgcacg gcgcgctttc gcgcgatgtc gcagcgcacg gccaagctgt acggtcagtt
     2521 gcatctgaac gtgaggcccg agcgcgcccg tgaagcgctg gagcagacgc ggcgccaggt
     2581 gcgcgccgac ctggatgagc tggaccgcca ggcctggccg cccgaggtgg cgcgtctgct
     2641 ggccgatgtg cgccgcagtg ccgagcgcct ggatgggctg acggcccagc ctgccggcaa
     2701 gagtggcctg caggccgtct cggcccaggc cgaccagatg ctgacccacg ccaacagtgc
     2761 cacgcaggcg ctggagcacc tggcgcaggc gccgacggcc aggctggtga acatggccgg
     2821 gcgccagcgc atgctgtcgc agcgcctggc caagaactat ggtctggtgg ccgcggggct
     2881 cgacagccgc cctttgcgcg agcagattgc gggtgatgcc gaggaattcc agcgtgccat
     2941 ggcgcagctt gccggcgcgc ccttgtccac gccggccatt cgcacgacgc tggagctggg
     3001 ccagggccaa tgggtgtttt tcgacatggc gatcaagcgc cagccgaatg cgcgcgggct
     3061 ggaggacgtg gccaccacta gtgaacgtct gctggaggtc atggacagcc tcaccgcgct
     3121 ctacgaagtg gctttgcagg atgtgctggg ctagccggct tccatgcctg aaattatgta
     3181 aaaaaatggc tgaaacgctt gtgtatcaag cgcaaagcgc tatttatttt gtagtttttt
     3241 gggctgttgg tagaagcgtg ggtaatgctg ccgcacgccc tcgccgtggt agtcccagcc
     3301 ggtgcactgg cccaggtggc gtggcggttc gtcgccacgg ctcttgaatg cggcgttgat
     3361 ggtctggaac gtggccgtgg ccaggttgaa cagcagctcg cgcagatgcg tgcagccggc
     3421 cacgccgccc aggtgggtct gtatggactg gcgccagccg cgcaccatgc tggcgcccac
     3481 catggattgc agggcgcgct gggcttcggg gcagccggcc agcgggtggg catccatggc
     3541 ggattcgatg gcgcgcacga ccaggtcgcc gtccaccgtg acgcgcagcc acatgtggtg
     3601 aatcggatca cccgcggggc gcagctgtcc ggggtggcgc agcgagggca ggtcgtaggt
     3661 cttgctgtcg tgcagttcgc cctctatgtc ccacaggcca tcgtcacgtt cgaagccctg
     3721 gtagttgacg cggcggatat gtctggcggt gcggggggag gggggtgaca ggggcatgaa
     3781 gaacggtttg aaactgcagt cggccatcag ccatggcgta tggccacggt cttcagggtg
     3841 gtgaagccgt acagcgcctc gaagcctttc tcgcgcccgt ggccgctgga cttgacgccg
     3901 ccaaagggta gttccacccc gccgccggcg ccgtagttgt tgatgaacac ctggccgctg
     3961 gccacgcggc gcgccatgcg caactggcgt gcgccatcac gcgtccacac cccggccacc
     4021 aggccaaagg gcgtggcgtt ggccagctcc acggcatggt cctcgtcgtg gaagggcatg
     4081 gcggccagca ccgggccgaa gacctcctgc tgcgccaggc ggtgatccgc gggcacgtcg
     4141 cgcagcaggg tgggggcctg gtagaagccg gtgtcgggcg cctcgtccac caccgtgccc
     4201 tgggcgacga ggggtatgcc ggccacctgc gcgtcggaca ggaagtccca cacacgctgc
     4261 tgctggcttt ggcgtatcag cggacccaga tcgagatcca tggccgcggg gcccacgcgc
     4321 agggactcga aggcctgggc cagacgctcc agcagcggct cgtagatcag cgaatcgatg
     4381 agcagccgcg agccggccga gcaggtctga ccgctgttct gcacgatggc gttgacgatc
     4441 accggaacgg cggcatccag gtcggcgtcg gcaaagacga tctgcgggct cttgccaccc
     4501 agctccagcg tcaccgggca atggcgctcg gcagccgcct gctgaatcag cgtgccaatc
     4561 ctgggactgc cggtaaagct gatgtggcgc acccccgggt ggcgcgccag tgcgtcgccc
     4621 acctcgtggc catagccggt cacgatgttg atggcgcctg ccggaaagcc cgcctcggcc
     4681 gccagctgcg ccacgcgcaa gagcgacagg caggcgtcct cggacggctt gactacgcag
     4741 acattgcccg cggccaatgc ggcggcgacg cagcggccaa agatctgcat ggggtagttc
     4801 cagggcacga tgtggccggt cacgccatgc ggctcgcgcc aggtgagcac gctgaagccc
     4861 tcctggtagg ggatggtctc gccatgcagc ttgtcgcagg cgccggcata gaactcgaag
     4921 tagcgcgcca gggccgcggc atcggcgcgc gcctgcctgg tcggtttgcc gcagtcgcgc
     4981 tgctcgatgg cggcgagctc gtcggcgttc tcggtgatct tggcggacag gcgctgcagc
     5041 agtcgcccgc gttcggccgg ggccaggctg cgccacacgc cgtcatagca ctgctgtgcg
     5101 gcgcgcacgg cggcatcgat gtcttccccg gtgccgcgct ggatctcgtc atatggctgg
     5161 ccgtcggacg ggtcgatgac ggcaatggtg cgcccggcgc tggaggctac ggcggcattg
     5221 gcgatgtagt ggtactgcat gcggcccatt gtgcggcaga tgggggctcg ccgctggcgc
     5281 cgttcgtgag ggcgggcatc gtcgggctca aacgcccaaa tcggcggatt atggttggct
     5341 agtgcgcact tggcgattgc gcctgcatgc tgatgccagt cgcaggggca atcggttgca
     5401 gccgtgtgag gctggcgtga agatgcgcca cggacgccgg atgcggggtg gccctggttg
     5461 ccagcctgat cagcgccgtt ccaaagctgt ggcatgcggg tgcgtttgcc ggccaggccg
     5521 gtggatcatg tgcaaggcat tgttcgaagg cattcaagag gagaccacca atgaccccca
     5581 acccttcgac gcacggcagt ccgctgcgca gctttttgat ctggggcgca gtggccatca
     5641 ttggcgcctg ggcgcttggt gtgatagcac tgcagcgtgg cgagccgatc aacgccgtgt
     5701 ggctgatcgt ggcggccatc tgcgtttacc tgattggcta tcgcttctac agcaagttca
     5761 ttgccgagaa ggtgcttgag ctggacgcca gccgcatgac gccggcctgg cgccacaacg
     5821 atggcctgga ctatgtgccg acagacaaat acgtcctgtt cggccaccac ttcgccgcca
     5881 ttgccggagc cggacccttg gtgggcccgg tgctggcggc ccagatgggc tacctgcctg
     5941 gcatgctgtg gattctggcg ggcgtggtct ttgccggcgc cgtgcaggac tttgtcgtgc
     6001 tgttcatctc gaccaggcgc gatggcaagt cgctgggtga tctgatcaag accgagatgg
     6061 ggccggtggc cggcgtgatt gcgctgtttg gtgccttcat gatcatggtg atcatcctgg
     6121 cggtgctggc gatgattgtc gtcaaggccc tgatccactc gccctggggc gtgttcacgg
     6181 tggccgcgac ggtgccgatt gccctgttca tgggcattta tctgcgtttc atccgcccgg
     6241 ggcggattgg tgaggtctcg gtgatcggct ttgtgctgct catgctggcc atcgtctacg
     6301 gccaggacgt ggcggccagc cccaccctgg ccccatggtt tgactacagc ggcgtcggcc
     6361 tgacctggat cctgatcatc tatggcgccg tggccgccat gctgcccgtg tggctgctgc
     6421 tggcgccgcg cgactatctt tcgacctttt tgaagatcgg cttcatcgtg ctgctggcgg
     6481 gcagcatcgt catcgtcgcg ccgcagatgc agatgccggc gttgacacgc tacatcgacg
     6541 gcaccgggcc ggtctggtcg ggcagcctgt tccccttcct gttcatcacc attgcctgtg
     6601 gcgcggtgtc gggcttccat gccctgatct cgtcgggcac taccccgaag ctgctggaga
     6661 acgaaaccca ggcgcgcctg atcggctatg gcggcatgct gatggagtcg gccgttgcct
     6721 cgatggccat gattgccgcc tgtgtcatcg agccgggggt gtacttcgcc atgaacagcc
     6781 cggcggcctt gatagggaca acacccgagc aggtggcaca ggtggtgtcg tcctggggct
     6841 ttgccgtgac accggaagtg ctgctggcca cggccaagga tgtgggcgag acgaccatca
     6901 tttcgcgcgc tggcggcgcg cctaccttgg ccgtgggcat ggcgcatatc ctgcaccagt
     6961 tgtttggcgg cgagggcatg atggccttct ggtaccactt cgcgatcctg ttcgaggcct
     7021 tgttcatact gaccgcggtg gatgccggta cgcgtgccgg ccgcttcatg ctgcaggatt
     7081 tgctgggcac ctttgttccg tcgatggggc gcaccgattc actgcccgcc aacgtgatct
     7141 cgacctgcct gtgcgtcgca gcctggggct tctttctcta ccagggggtg gttgatccgc
     7201 tgggcggcat caacacgctg tggccgttgt ttggcattgc caatcagatg ctggcggcgc
     7261 tggcgctgac gctgggcacc gtggtgctgt tccggatgaa gaaggatcgc tacgcctggg
     7321 tcactgtcgt gcccactgtc tggttgctgg cttgtacgct gactgccggc tggatgaagc
     7381 tgttttcagc cgacccgcgc gtgggttttg ttgccacggc gcgcaagttc agcgccgagg
     7441 tcgccaggca ccaggccgag ggaacgctgc cggagaaggg gcccacgatc gagcagatgc
     7501 agcagatcgt tttcaacaac tacctggatg cagccctatg cgccgtcttc atgctggtgg
     7561 ttgtatcggg agtgatcttc ggcatcaagg ccgtgctgca tgcgcgttcg cacaacaaac
     7621 cgagcgtgcg cgagacacct taccagccct tgccggccac tggagcctga cgccatgcca
     7681 ggtttggtta ccctcagcag cctggcgcat gccgcgcaag aaagcggcag ctacctggca
     7741 cgggccgcgc gcatgatggt gggtgtgccc gactacggcc agtatctggc gcacatgcgt
     7801 cgcgaacatc cgcaggaggc gccgctcaat tacgaggagt tcttcaagaa ccgcatgcag
     7861 gcccgctacg gcggcggtcg cgcgggttgt tgttgaggtc gatcccaact cggccatcgg
     7921 cctggccagc caatgaaaaa cccgcttgaa gcgggttttt ctgtggttgt cgacctggtg
     7981 gcgcctacat caataccact tccacgcgac gcgcctgtgc tccgctgccg gcctggatgt
     8041 cggcgggctt ttgcagctca acctgatcct cggcgacgcc caggcccatc agcaagtcgc
     8101 gtaccgccaa ggcgcgttgt ttggctattt cggcattggc tgcagcgctg ccggtgctgt
     8161 ccacatagcc actgatgacc gccttcttgc cggccttgac cccgtccacg atgcgtgcca
     8221 gagcccccgc gccatcggca tgcggctcgg ccttgccgct tgcgaaatag aagatcacct
     8281 tctcgttctc aatgacgacc gaggccgcat cgtcggccgt ggctacggcc gcggtgggcg
     8341 cggccggtgc cgtggccgcc accttggtgc caccatgcat ggccaccacc agcgggacga
     8401 tgagcagggc cacgatgttg atgatcttga tcagcgggtt gactgccggg ccagccgtgt
     8461 ccttgtaggg gtcgcccacc gtgtcgccgg tgacggccgc cttgtgcgcc tcgctgccct
     8521 tgccgccgtg gtggccatcc tcgatgtatt tcttggcgtt gtcccaggcg ccgccgccgg
     8581 tgcacatgga aatcgccacg aacaggccgg tgacgatggt acccatcagc aggccgccca
     8641 gcgccttcgg ccccagggcc aggcccacga cgatgggcac ggccaccggc agcaggctgg
     8701 ggatgatcat ctccttgatg gcggcgctgg tcagcatgtc cacggcgcgg ccgtattccg
     8761 gcttggccgt gccttccatg atgcccttga tctcggagaa ctggcgccgc acctccacca
     8821 ccacgctgcc ggcggcgcgg cccacggcct ccatggccat ggcgccgaac aaatagggaa
     8881 tcaaaccgcc gatgaacagg ccgacgatga ccatggggtc ggacagatcg aacttcacag
     8941 cctggccgta gctgtccagc ttgtgcgtgt agtcggcgaa cagcaccagg gcggccagac
     9001 cggccgagcc aatggcgtag cccttggtga cggccttggt ggtgttgccc accgcgtcca
     9061 gcgggtcggt gatgtcgcgc acgctgctgg gtagctcgct catctcggcg atgccgccgg
     9121 cgttgtcggt gatcgggccg taggcgtcca gcgccaccac gatgcccgcc atggacagca
     9181 tggacatggc cgccacggcc acgccataca ggccggccag catgtacgac acgatgatgg
     9241 ccgcgcacac aaagagcacc ggccaggcgg tggagcgcat ggacacgccc agcccggcaa
     9301 tgatgttggt gccatggccc gtggtcgagg cctgggcgat atgctgcacc ggcgaatact
     9361 gcgtaccggt gtagaactcg gtgatccaca ccagcgcggc cgtcagcacc aggccggtgg
     9421 cgcaggcgcc aaacagccgt attgcagcgc cgctgccgcc cagggcgttg tcgggaatga
     9481 tccaggcggt gacgaaccag aaggcgacca gcgagagcac gcccgcaatc gccaggccct
     9541 tgtagagcgc aggcatgacg ttcttcatgc cggggctggc cttgacgaag aagcagccga
     9601 tgatggaggc gacgatggac acggctccca gcgccagcgg ataggcgacg gccgccgtcg
     9661 gcgcggcgcc tatgagcagc gcgcccagca ccatggtggc gatcagcgtc acggcgtagg
     9721 tctcgaacaa atcggccgcc atgccggcgc agtcgcccac gttgtcgccc acgttgtcgg
     9781 cgatcacggc ggggttgcgc gggtcatcct cggggatgcc ggcctcgacc ttgcccacca
     9841 ggtcggcgcc aacgtcggcg cccttggtga agatgcctcc ccctaagcgc gcaaagatgg
     9901 aaatcaggga tgagccaaag gcaaaaccga tcagggggtt cagcgtggcg gccagctgcg
     9961 cgtccgcacc cgagcggccc agcaggaacc aggcaaagcc ggtcacccct agcaggccca
    10021 ggcccaccac cagcatgccg gtgatggcgc cgccacgaaa cgctacatcc agcgccggcc
    10081 ccatgccctg tgtggcggcc tgggcagtgc gcacgttggc acgcaccgag acgttcatgc
    10141 cgatgaagcc gcaggcgccc gagagcacgg cgccgacgac aaatccggcg gcggtgagcg
    10201 tgtccaggaa caggccgatg agcacggcaa gtaccacgcc cacgacggca atcgtcttgt
    10261 attggcgcgc caggtaggcc gcggcgccgg cctggatggc ggcggcgatc tcttgcatgc
    10321 gggcgttgcc cgcgtccttg gacagaatcc agctgcgggc ccaaaggccg tacaccacgg
    10381 cgagcaagcc gcacgccaaa gccagtatca ggggggaagt caatgctgct tgaccagcca
    10441 tacgcgatct ccatgggcaa ggggtcacac gggtggaagc gcaacgccgg ggaagtgctt
    10501 ccgctgcgtt gcttcctcgc catgccgatg ccgcaaacag cctggagacg attggccaac
    10561 gggggcaatt tactgcgaaa ccccggcctg caagctggct gcaagccgcg tcgatctaaa
    10621 atccagggtt aatcccgagc cgctggtgct acgacctgca cggccattgg cgcgggagcc
    10681 acctcgtatc aaccactcca acctccaaac atgtcgctga acaacgtcaa gcctggcacc
    10741 aacatccccg attccttcaa cgtggttgtg gaaattccgg ccgaatccga tcccgtgaaa
    10801 tacgaggtgg acaaggaatc cggcgccgtc ttcgtggatc gcttcctgac cgtggcgatg
    10861 tactacccct gcaactatgg ctacatcccc cagacgctgt cgggcgacgg tgacccggtg
    10921 gacgtgctga tcatcacccc ctatccgctg ctgcccggcg tggttgtgcc ctgccgtgca
    10981 ctgggcatcc tgaagatgga agacgaagcc ggcgtggatg gcaaggtgct ggccgtgccc
    11041 accaccaaga tcctgccgat gtatgaaaac tggaagaccg tggaggacgt gaaccccatg
    11101 cgcctgaagg ccatcagcca tttcttcgag cactacaagg atctggaaga cggcaaatgg
    11161 gtcaaggtgc tgggctgggg cggcatcgat gccgcgcaca aggaaatctt ggacggcgtg
    11221 gagagctaca agaagggttg aacccggggc agccctgggg ctgcttatcc gcattgcgtg
    11281 aaagcctgcc ggcagtgttt gccggcaggc ttttttgttg tcgagtgtgg acgcgacggt
    11341 gatctttagt aggtttttgg tagaaatgtc aaaataagta acgttcagct ggtattctgc
    11401 tgagacacac ggggtcaggc ggcgttgata tccaccggcc ctggtgcttg ccaaataccg
    11461 ttttcctagg agttgccatg ttgtctgcca ccgcccagcg tccttccacc agcaatgccg
    11521 ccacgagcaa cgctgcggag tacctgacct tccgcctggg gcaggaagag tacggcatcg
    11581 acatcctgcg cgtgcaggaa atccgctcct acgagcaacc cacgcgcctg gcccatgcgc
    11641 cggacttcat caagggcgtg atcgacctgc gtggcgtcat cgtccccatc gtcgatctgc
    11701 gcctcaagct gcactgcgcg caggccgaat acaccgattt caccgtggtc atcatcctca
    11761 acgtgggcgg caccgtgctc ggggcggtgg tggacgccgt ggccgacgtg gtcgccctgc
    11821 aggccgaggc catcaagccc gccccgcagt tccagggcca ggtggacagc agtttcgtgc
    11881 gcggcatcgc caccgtgggc gagcgcatgc tcatcgtgct ggacattgag tctttgctca
    11941 gcggttcgga aatggggttg gtgcaggccg ctgctatggt ttgatgcggc cgtcctgatg
    12001 ggcgcgcctg aacgggctgc ggttgtccag atcttccagg tagtgctgca cgccttggcc
    12061 ttcgcgcgcc agaaaatgcc ccacggcctg ggcaaaggcc ggatgcgcca gccaatgcgc
    12121 actgccggcc tgcaccggca tgagggcgcg cgccatcttg tgctcgccct gggccccgcc
    12181 ctcgaagcgg cgcacaccct gggcgatgca ccaggcgatg ggctggtagt agcaggcctc
    12241 gaaatgcagg cagtccacgc gctccagcgc gccccagtag cggccatagg ctgttgatgt
    12301 caagctattt tcgcttctaa cgcttgatgg gtaagcgctg acagctatca aactgctagc
    12361 tatccgttgg ccgccgcgct cggccacgaa cagcacccag gcctcgggca tctgctcctg
    12421 catcaacgta aaaaacccgc gcgtgaggta gggcgggttg ccatgctcca ggtaggtgcg
    12481 ctggtagcag tggtagaaaa aatcccagtc ggcggccgtg atctcacgtc cgcgcagcac
    12541 gcgaaacact acgccggcct caagcacgcg gcggcgctcc tggcggattt tcttgcgctt
    12601 gtcctgggtc agcgacgcca ggaaatcatc gaaatcggcg aacctggcgg cattttgggg
    12661 atgccggttg tgccagtgga actgcacctg gtggcgcagc agcaggccgg ccgcgcgtgc
    12721 ggtggccagc tcgtcgtcgg ccgcgaacag caggtgcagc gacgacaggc cgctttcacg
    12781 cgcccagtcc agcaaggccc gcagcagcgc cgtccgcgat gcgtcgccct gggccagcaa
    12841 gcggcttccg ggcacgggcg tgaagggcac ggccaccacg gccttcgggt agtagggcag
    12901 gccatggcgc tcatgggcgt cggcccaggc ccagtcgaag acatactcgc catacgaatg
    12961 ggccttgatg tagagagcgc aggcggcgca cagccgctcg ccctgccaca gcgtgaaata
    13021 gcgcggcacc cagcccgtgc gcgccgtggc gctgccgctg gtttcgagtg cggccagata
    13081 ggcgtggcgc atgaagggcg tgggctggga ctggagcgcc agcagggcgt cccaggcggt
    13141 ggcgtcgacc tggctggccg catcgtgcag ccgcagttcg taggggggcg tggtgctggt
    13201 caagggcagg gcgtaggtgg tggggggccc atcatggcgc caaaaccgct ggcccgcgtg
    13261 gcatgggggc tgcgggcata atcaagcgcg ttttttcgcc tttctcccta tgtccttcgc
    13321 catcagcatc gcccagctca atttcgtggt gggcgacctg ccgggcaacg cccgcaagat
    13381 cattcgcgct gcccaggatg cccacgcggc cggagccagg ctgctgctga cgcccgagct
    13441 ggccctgtgc ggctacgccg ccgaggatct ttatctgcgg ccggcgtttc tcgcagcctg
    13501 tgagcaggcc ctggccgagg tggtggcggc gacagcggcc ttgcccggcc tggctgtggt
    13561 ggtgggccat ccgcagcgcc tgccgcatgg gatcggcgaa ggcggccgca acctgtgcca
    13621 caacgccgcc agcgtgttgc gcgcgggccg catcgagcaa acctacgcca agcaatacct
    13681 gcccaactac gaggtgttcg acgagcgtcg ctacttcgtg ccgggcactg acagctgcgt
    13741 gttcgaggtt gatggcctgc gcctgggcct gctgatctgt gaggatgcct ggtatcccga
    13801 gcccgcccgc cgcgcgcgcg aggccggggc gcagatgctg ctcaccatca atgcctcgcc
    13861 ctaccacctg ggcaagggtg gcgagcgcga gcaggtgatg cgcgagcgcg tgcaggaaac
    13921 cggcctgccc ctggtctacg cccacctggt gggcgggcag gacgaggtgg tgttcgaggg
    13981 gcgctcgttt gctctgaacg ccgatggcag cgtggcggcg cgtgcgccag gatttgaaga
    14041 aaaaatgctg tttgctcagg tacagcaagc gcaagcagct attactattg aagcagatgt
    14101 ggcgccacgc gaaggctggg aggccgaact gtgggcggcc ctggtgctgg gcgtgcgcga
    14161 ctatgttggc aagaacggct ttcccggcgt gctgctgggg ctgtcgggcg gcatggattc
    14221 ggccctggtg ctggcgattg ccgtggacgc gctgggggcg gacaaggtgc gtacggtgat
    14281 gatgccctcg ccctacaccg ccgacatcag ctggatagac gcgcgcgaca tggccgagcg
    14341 cctgcaggtg cgctacgacg agatcgccat cgcgcctcag ttcgaggcct tcaaggccgc
    14401 gctggcgccc cagtttgccg gccgcgccga ggacactacc gaggaaaacc tgcaggcgcg
    14461 catccgcggc acgctgctga tggcgctgtc caacaaattc ggcagcgtgg tgttgaccac
    14521 gggcaacaag agcgagctgg ccacgggcta ttgcacgctg tatggcgaca tggccggcgg
    14581 ctttgccgtg atcaaggatg tgctcaagac gcgcgtgttc gcgctggcgc gctggcgcaa
    14641 tgcccacgac ccctatggca cgggcgccaa tcccatcccc gagcgcatca tcacccgccc
    14701 cccgagcgcc gagctgcgcc ccgatcagac cgaccaggac aacctgccgc cctacgaggt
    14761 gctggatgcc atcgtcgagc gctacatgga aaacgatgaa cccgtcgaag acatcgtgcg
    14821 cgccggcttt gcacacgccg acgtggagcg cgtgacccgc ctcatccagg tcaatgaata
    14881 caagcgccgc caggcacccg tgggcgtgcg cgtcacgcgc cgcagctttg gcaaggactg
    14941 gcgctatccg atgacgaaca aattcagggc ctgacgcagt ccgggccgca acgattcata
    15001 tccaggagtt ttcaccatgc aaatgattac cgccgtcatc aagcccttca agctggagga
    15061 ggtgcgcgaa gcattggccg aatgtggcgt gaccggcctg acggtgaccg aggtcaaggg
    15121 ttttggccga cagaaaggcc ataccgagct ctaccgtggc gccgagtacg tggtggactt
    15181 tctgcccaag gtgaagatcg aagtggtggt gcgcgaccag gatgtggatc gctgcgtgga
    15241 cgccatcgtg ggcgcggcgc gcaccggcaa gatcggtgac ggcaagatct ttgtgacggc
    15301 ggtggagcgc gtggtgcgca tccgcaccgg cgagctggac gactcggcgg tctgaaccac
    15361 cgccgtcaga tcacgtcgcg cagcgggatg gtggtggcgt tcaggccggc atcctgcacc
    15421 agggcagcga gcagcccggc gggttcggtg ccggtgcgaa tcaggcccat ctgccactcg
    15481 ctcatcaggc cgttcttcac ggcatgctgc atgaaggcca tcaggccgct gtagtagccg
    15541 tcggtgtcca gcaggcccac gggcttgtcg tggtagccca gctggcgcca ggtccagacc
    15601 tcgaacagct cctccagcgt gcctatgccg ccgggcaggg ccaggaaggc gtcgctgcgc
    15661 tcggccatca tggccttgcg ttcgtgcatg ctcgtgacca catgcagttc gtcgcacagc
    15721 tggttggcca actccttgtc caccagcgcc tgcgggatga tgcccaccac gcgtccgccg
    15781 gccaggcgcg tggcctcggc caccgtaccc atcaggccgc tacgcccacc gccatagacc
    15841 agctgcccgc catggcggcc aatccagcgg cccacggcct gtgcggcttc ggtgaacaaa
    15901 gggttgtcgc cctggcgtga gccgaggtag acgcatacgg aaaatgccat atcagccatc
    15961 aggaaaacct ttggaaaagc gccgcagccc agacagcgcc gcacagcagc aggctgagca
    16021 gcacggcggc actgcccatg tccttggcgc gcttggacag gtcgtgcaac tcggggccta
    16081 tgcggtctat ggccgcctca atgccggagt tgagcagctc ggtgatcagt accagcacca
    16141 cgctggccgt cagcaaggcg gtttcgaccc agccgcggcc cagccagcac gctagcggca
    16201 acagcacggc ggccagtaag acctcttggc ggaaggcctt ttcgcgccag cccgcgcgca
    16261 acccggccag cgaatagccc gtggcatgcc acaggcggtt caggccggtg cgctgcttgt
    16321 gtgggtgggg ggcgggggta tcgggcatgg cggtgcgggt gcgtcttatt ttttcttgcg
    16381 tggcgccggc ggcagttcgt ggtgcaccag gcgcgtgccc gccagcgcat cgtgccagaa
    16441 ctggcgttgc gggtgcaggc ggctgagcaa tgcccagatg gccacccagc cgagcacgat
    16501 gacggccgat gcgccgcccg agaggtggta gggcgcgatc accgccaggg gcggcacgaa
    16561 ccagatccag ctcagcatat agcgcagcag ggcgcgcttt tggctgatga gccggccctg
    16621 gcgatcgacc acgcggatat gccaggtctt catggccagg gtatggccgc gcgaccagaa
    16681 ccagacgaaa tagatgccga agacaatgaa caggaaggcc tgcagcccat ggcggttgtc
    16741 cagggcgttg cgtgtctgtg tcagtgtgcc gaacaggtag ccggcgatga acaccacgcc
    16801 gaacatgagc atgccctcat agagccagca ggccatgcgc cggtgcagcg gcggggccat
    16861 gccttcggtt ggcggtgatg ttggggcagg gggcgcgcca gtggtggttt ttgacgggcc
    16921 tggcgggctg gacgtggaca tgcgggagcg gggcggcaag cgtggttatt ccggaggatg
    16981 cagcggcgga tagtaacgcg ccggctcttc cttggcctcg gatgcgcccg aggcgggctc
    17041 cagcggcgcc agcggcgcgg ccacggccga gggggtgttg ataggggccg tctcgacggc
    17101 gatgggcggc ggcggcaggg tgatcggcgc cgggtgcaga accgtgggcg ccacgatttt
    17161 gggcgggttg agcacggccg gcggggcctc tgtggcagcc ggcacgcgtg ccagtttcct
    17221 gggcgacacc gggtggatgg ccgtggctgc tccacgcggc ctgggtgcgg ccctggcggc
    17281 cagccgtttc ttttcctcgg cgctcagcgc ctggtaggcc tcccactggg cgcgcctgtc
    17341 gtcggtgggc agcttggccg cggcggcgta gttcaggcgt gcctggctgc gttgttgggc
    17401 gctcagactg gcccagtcgg tgatgcgctc cagcatcttt tcctgctcct ctggggtgag
    17461 agcatggaat ccggctgcca gattcaacca gttgcgtttt tgcaactcgc tgagcaggga
    17521 ccagcgattg gccagcggat acagagcctc cttctggcgc gtggtcaggg tggaccaggg
    17581 cgggccgacc tcgggttgcg gggcaaaccg catctcgcca aactttccgc cactcagagc
    17641 ctgcgcaggc ataggggtac tgggcgcaat acgggtatag gccgtcatcc accagccgcc
    17701 ggcggccagc acgcccagca acacaaaagc cagcacgaat gctggcagtc tgtgggcggg
    17761 ggtacgggga ggagctaggt gcatgcaggc gctgggtttg cgggtcggcg gattacgggg
    17821 tctggtgcga tgacttcaga aattgcacaa aacccggatc ggcgtaggcg gaggggggca
    17881 ggtcgcccgt cagcaacgcg gcgtccactt cggcaatctc gctggcaacg gcatcatcct
    17941 gctccatatt cacgacaaac aggccaatga ccagggccgc caaagggacg gcggacatca
    18001 ctgcgcgcca ccagttgcca ccctcgcgcc cccagccacc gagcgaggcg ctggcgccat
    18061 ggacaagaat ctgcgtcgcg gattcggtct gctgcaccgt ttgaatggca ggccgcttgc
    18121 gctgggccag ggcctgcatg cgcgcggcgc gcaggcgctc ggtgatgtcg tagggcaggt
    18181 cggcattgcc gtgtgtcagg cgcgccgtca ggcgccgcgc gtaggcgtcg gcagcctgat
    18241 tggcttgcat gctgggttct gtggtgcggt tcatagcacg attcctttgg ccttcagtgc
    18301 cttgctgagg gtttgcacgg cgcgaaaaca atgtgtcttg acgctgcctt ccgagcagcc
    18361 catggcggcg gctgtttctg cgacatccat ttcctcccag taacgcatca aaaacgcttc
    18421 acgttgacgt gcgggcaaca gttgtatctc ggtttcgatg gcacgcagga cctgggcgcg
    18481 ccgcaggctg tcctcggcgc tttgcgcggt ctcgccttcg gggacgcccg catgcacctc
    18541 aaggaaatcg aaatccgcgc cgtcctcgcc cgggccttca aagtcgctca ggctggagaa
    18601 cagggcattg cgcgtctttt gccggcggaa ccagtccagc gtgcagttgg tcagaatgcg
    18661 ctggaacaac atgggcagtt cgcccggcgg tttgtcgccg taatgttcgg ccagcttgag
    18721 catgctgtcc tggacgatgt ccagggccgc ttcctcgttg cgaacgtggt acagcgtgcg
    18781 cttgaaggca cgcttgtcca cgctcttgag gaagtcggat agctcttgtt cggtggccaa
    18841 aaggcaaaag acccgcgaag ggcctgctgt gacggtcggg cgggcactgg gctcgtcgat
    18901 aagcgggcaa ttatcgcatt gcgtgcatgt ttttttgtgg catgacgcgc cagaccttgt
    18961 gtgcagtgca ataatctgcg ctgcaaatca aaaaggcaaa cgggtctcgg ttcggcgttc
    19021 actcctctcg ctcatggctg aggcctcaag tcccaagtcg gctccataag agtgcgtaca
    19081 aggggcaccc aaggtttttc gttagagaaa ttcgcaaagg ttgatcatgg aaatctccaa
    19141 ggccgagctg acctcggcag cagcagcatc gcaggcatcg ggctcccagg agctcatggg
    19201 tgccgaaatt ctcgtcaagg ccctgcaggc agagaacgtg cagtacatct ggggttaccc
    19261 cggtggcgcc gtgctccaca tctacgacgc cctgtacaag caggaaacca tgcagcatgt
    19321 gctggtgcgc catgagcagg cggccgtgca tgcggccgac ggctacgccc gtgccaccgg
    19381 cgacgtgggg gtggcgctgg tcacgtctgg ccccggggtg acgaatgccg tcaccggcat
    19441 cgccacggcc tacatggact cgattcccat ggtcatcatc accggtcagg tgcccacgcc
    19501 cgcgatcggg ctggatgcct tccaggaatg cgacaccgtg ggcatcacgc gccccatcgt
    19561 caagcacaac ttcctggtca aggacgtgcg cgacctggcg ctgacgatga aaaaggcctt
    19621 ccacatcgcg cgcaccggcc gccccggccc ggtggtggtg gacatcccga aggatgtttc
    19681 cttcaagaag gcgccctaca acggttaccc caagagcgtg gaaatgcgct cgtacaaccc
    19741 ggtcaagaag ggccatgcgg gccagatccg caaggcactg cagctgttgc tgacggccaa
    19801 gcgtccctac atctacaccg gcggcggcgt gctgctgggc aatgccacgc aagaactgcg
    19861 caccctggtg gacatgctgg gctacccggt cacgaacacg ctgatgggcc tgggcgcctt
    19921 tccggccacc gacaggcgct tcgtcggcat gctgggcatg cacggcacga tagaggccaa
    19981 caacaccatg cagaactgcg acgtgctgct ggccgtgggc gcgcgttttg acgatcgcgt
    20041 catcggcaac cccaagcatt tcgcgcagaa cgaccgcaag atcatccata tcgacattga
    20101 tccgtcgagc atctccaagc gcgtgaaggt ggacatcccc atcgtcggcg acgtcaagga
    20161 ggtgctggcc gagctgatcg ccatgatccg cgagaccacc acccgcgccg atgctggcgc
    20221 cctggccgcc tggtgggatc agatcgagtc ctggcgcggc cgcgactgcc tgaagtacga
    20281 ccggggcaac accgaggtca tcaagccgca gtatgtggtg gagacgctgt ggaacatgac
    20341 gcgggatgtg gaggcctaca tcacctcgga cgtgggccag caccagatgt gggccgcgca
    20401 gttctacaag ttcgatgagc cgcgccgctg gatcaactcc ggcggcctgg gcaccatggg
    20461 cgtgggcatt ccctacgcca tgggcatcaa gctggccaag ccggactccg aggtcttctg
    20521 catcacgggc gagggctcgg tgcagatgaa catccaggag ctgtccacct gcctgcagta
    20581 caacacgccg atcaagatcg tctcgctgaa caaccgctac ctgggcatgg tgcgccagtg
    20641 gcaggagatc gagtactcgg gccgctacag ccacagctac atggacgcgc tgcccaactt
    20701 cgtgaagctg gccgagtcct atggccatgt gggcctgctc atcgagcgcc cgcaggacgt
    20761 ggagccggcg ctgcgcgagg cgcgcaagct caaggatcgc acggtgttcc tggacttccg
    20821 taccgaccct tccgagaacg tgttccccat ggtgcgcgcg ggcaagggca ttaccgagat
    20881 gctgctgggg tcggaagatc tttaaggcaa atcggcaccc agcgcttatc ctataagcgc
    20941 aagcagctat tcattgaata gcattatttg acgaatctat tgcctgccaa gcctggcgct
    21001 taccggcgcc aggggagggc agcgaaaaac gacagttttc tgcgaccatt tttttgatcg
    21061 tgctgttgtc gcaaaaagag gaagcacatt tcatgaaaca catcattgcc gttctgatag
    21121 agaacgaagc cggcgccctg tcgcgcgtgg tggcgctttt ttccgcgcgt ggctacaaca
    21181 tcgagtcgct caccgtcgca ccgacggaag acgcttcgct gtcgcgcatg accatacaga
    21241 ccacgggctc ggacgacgtg atcgagcaga tcaccaagca tctgaaccgc ctcatcgagg
    21301 tggtcaaggt ggtggacctg accgaggggg cgtacaccga gcgcgagctg atgatggtca
    21361 aggtgcgcgc cgtgggcaag gagcgcgagg agatgaagcg catggccgac atcttccgcg
    21421 gccgcatcat cgacgtgacc gacaagagct acacagtcga actcaccggc gaccagtcca
    21481 agaacgacgc ctttttgcag gccatagacc gcacggccat cctggagacc gtgcgcacgg
    21541 gtgccagcgg cattggccgt ggcgagcgca ttctgcgcgt gtaagacaat ccaccgtttt
    21601 ctttttttga acagaacctc aaccggagcc aacatgaaag ttttctacga caaagactgt
    21661 gacctgagcc tgatcaaggg caagaccgtg gccatcatcg gctacggcag ccagggccac
    21721 gcccacgcgc aaaacctgaa cgacagcggc gtgaaggtcg tggtcggcct gcgcaagggc
    21781 ggcgccagct gggacaaggt cggcaaggcc ggcctgaacg tgctggaagt gaacgacgcc
    21841 gtgaaggccg ccgacgtggt catgatcctg ctgcccgacg agcagattgc cgaggtgtac
    21901 aagaacaacg tcgagcccaa catcaagcag ggtgcgtcgc tcgcctttgc ccatggcttc
    21961 aacgtgcact acaaccaggt cgtgccgcgc gccgatctgg acgtgtggat ggtggcgccc
    22021 aaggcccccg gtcacaccgt gcgcaacacc tacacccagg gcggcggcgt gccccacctg
    22081 gtggcggtgc accaggacaa gtcgggcaag gcgcgtgacc tggccctgtc ctacgccatg
    22141 gccaatggcg gcggcaaggc cggcatcatc gagaccaact tccgcgaaga gaccgagacc
    22201 gacctgttcg gcgagcaggc cgtgctgtgc ggcggcgccg tcgagctgat caagatgggc
    22261 tacgagacgc tgaccgaagc cggctacgct cccgaaatgg cctacttcga gtgcctgcac
    22321 gagctcaagc tcatcgtcga tctgatctac gaaggcggca ttgccaacat gaactactcg
    22381 atcagcaaca acgccgagta cggcgagtac gtgaccggcc ccgaggtgat caacgagcag
    22441 tcgcgcgtgg ccatgcgcaa tgccttgaag cgcatccagg acggcgagta cgccaagatg
    22501 ttcatccagg aaggccgcct gaactacccc agcatgacgg cacgccgccg caacctggcc
    22561 gcgcattcca tcgagcaggt gggcgcccag ctgcgcgcca tgatgccctg gattgccaag
    22621 aacaagctgg tcgaccagag ccgcaactga ttgccaaagg cgcggccccg ggcagcgcca
    22681 cggtgatgca caaggccacc tcggtggcct tttttgcgtg tgccggcaag ggctacactg
    22741 ctgcctctcg cacccctggt gcaatcctga atactattaa atatatagct gtttgcgctt
    22801 tattgacggg cgctggaggt cattttgatg catgatggcg acgaagccac cgaaaacacc
    22861 ggggtcttgg tgcacaagcg ccgcaagggc atttacgtgc tgcccaacct gttcacgctg
    22921 gcggcgctgt ttggtgggtt ttacgccatc gtcatggcca tgaacgggcg tttcgaactg
    22981 gcgtcgatgg gcgtgttctg cgccatggtg ctcgacagcc tggacggccg cgtggcgcgc
    23041 atgacgaata cgcaaagcgc ctttggcgag caaatggatt cgctgtcgga catggtgtcc
    23101 ttcggcgccg cgccggcgct gatcgcctac atgtgggcgc tgcagggcct ggggcgctgg
    23161 ggctggattg cggccttcgt ctactgtgcc tgtgccgcgc tgcgcctggc gcgtttcaac
    23221 gtgaacacgg gggtggtgga caagcgcttc ttccagggcc tgccttcgcc ggcggccgcc
    23281 gccctggtga tgggtttcat ctggctgctg accgatgccg gcgtgcaccc ggggcaggac
    23341 ttgctgggcc tgtcctgggt gcatatcacc tgggtgatgt tcggcttcac gctctacgcc
    23401 ggcctgacca tggtcaccaa tgtgccgttc tacagcttca aggatctgca catgaagaaa
    23461 agcgtgccct ttgccgccat cgtgctcatc gcgctgggca tcgccgtggt caacatccac
    23521 ccgcccacgg tgctgttcgg catctttgtg ctgtacggcc tgtcgggcta cgtgctgtat
    23581 gcctggcgca aggccaaggg ccagcacacg agtgtgatca gcacctcgac cgacgaaccc
    23641 gacgagcagg ggctacacca ctgatcccct ttactcggcg tccggctgcg cgcttggcgc
    23701 ggcttgttgg aaggccggca gttgctcgca ggcagcgacg atgcgcgtga tggtgggata
    23761 ggcatcgagc gcgcagttga agcgcctggc gttgaacacc tggggcacca ggcagcagtc
    23821 ggccaggccc ggggtgtcgc catggcagaa gcggccggtg tccgggctgc gcgccaggct
    23881 gtcctccacg gcctgcaggc ccagcgccac ccaatgggcg taccaggcgt ccttttgcgc
    23941 gcccgacacg cccagctcct gcgtcaggta ctggagcacg cgcaggttgt tcagcggatg
    24001 gatctcgcag gccaccattt gggccagcgc ccgcacgcgc gcgcgcccca gggcatcggc
    24061 gggcagcagg gcgggctggg ggtgtacctc gtcaaggtat tccatgatgg ccagcgactg
    24121 cgccagcacc gggccgccca cggcgggttg caatgccggg accagcccct gcgggttggc
    24181 gctgcggtag ccgctggcgt gttgctggcc gccatccttg accagatgca ccgggacggt
    24241 ttcgtaggcc aggcccttga gcgccagggc aatgcgcacg cggtaggccg cggagctgcg
    24301 gaagtacgaa tacagccggg gcagggcgcg cgcacccgcc tggtgcgcgc tggcgccggc
    24361 gggcgtggcc ggccgctcgg gcagggttgc gagctcaggc ggcgccatgt gtgtccacct
    24421 cgttctcaat ctggccaaag atgctctggc cctggccgtc gaacatttct atgcgcaccc
    24481 tgtcgccatg tttcatgaag ggggtggcgg gcttgccctg ctctatcgtc tcgtaggtgc
    24541 gcacctcggc caggcagcag tagcccatgc cgccgttggc gatcgacgag ccccacaggt
    24601 cgccctgctt gttgctcacc gtgcccgagc ccacgatgga gccggcgcag agtgtgcgtg
    24661 tcttggccac atgggccacc agctggccaa agtcaaacgt catgtccacg ccggcgttgg
    24721 gcttgccaaa cagctgctcg ttcaggtgca cgaccagggg caggtgcacc ttggcgtcct
    24781 tccacgcctc gcccagttcg tcgggcgtga cggccacggg gctgaaggcg ctggcgggct
    24841 tgctctggaa gaagccgaag cccttggcca gctcggccgg gatcaggttg cgcagcgaca
    24901 cgtcgttgac cagcatcacc aggcggattg ccttggcggc ctgctcgggc gtggcgccct
    24961 ggggaacgtc gccggtgacc accgtcacct cggcctcgaa atctatgccc caggcttcct
    25021 gggccagcac cttgccgcgc ggcgggatga agccgtcgct gccgccctgg tacatcagcg
    25081 ggtcggtgta gaagctctcg ggcacttcgg cgttgcgtgc cttgcgcacc agctccacat
    25141 ggttgatata ggccgagccg tcggcccatt gccaggtgcg cggcaggggc gagtggcagg
    25201 cgttctggtc gaaggcctcg ccctcgatgg cgccgctgtt cagcgcctcg tagacctggc
    25261 gcagctgcgc ctcgacggtg gcccagtcgt ccatggcggc cagcatggtg cgggcgatgg
    25321 cgggtacggc gcggcagcgc ttcaggtcac ggctgacgac caccagggtg ccgtcacggc
    25381 cgccttgctt gagggttgcg agtttcatga tgtgagtcct ttccttgtga tgtcgggtgc
    25441 ggcgttcagg ccgccttggc gttggtgctg ggcgtgtcgg cggcgcgtcg accgcggata
    25501 tggtgcttga ccaggaatag cgccacgagt gccgcaagca tggcatagcc cagcagcgac
    25561 agatagccct gcgcgccatt gctggtgatg atggcggcgc cggcgaacga gctcaggatg
    25621 gcgcccaggc gaccaaaggc caggccagcc gcggtaccgg tggcgcggat atgggtggcg
    25681 tagacatggg cgcacagcgc atacatgggc gcctgcacgc cgttgacgaa catgccgtgc
    25741 acgcccaggc ccagcatcag caggctggcg tcctggtgga agtccacgcc cttgagtgcc
    25801 agggcgctgg ccgcggcccc ggcgcaggcc agcgccagtg gccagcgcga gccaaagcgc
    25861 gacatggccc aggcgcagcc cagggcgccc agcacgccgc ccaggttgta ggccgtcagg
    25921 cccgagctgg ccatggagac cggcagcccc tcggcggtga gcatcgatgg cagccagctg
    25981 aaggcgctgt acgaggccag catgaccatg aagaagccca tccagatggc cagggtgtcg
    26041 cgcgccaggc cgtccttgaa cagggcgcca aaaccggcgt tctgcttctg gccggccgcc
    26101 tgctccacgg catcgacaaa gggcgcgtct gccgccgtgg ggcgctgcat gcgcgccagc
    26161 agggtgcgca gctggggcca ctgggcgctg tggcgcgcca tcaggcgcgg tgattcgggc
    26221 aggcgtgcca gcaggaagaa gctgtaggcc agcggcagcg cgccgccgat ccagaacatg
    26281 gcgcgccagc ccaaagtggg cagcacaaag ctggcgaaca gtccggccag catgccgccc
    26341 agcgggtagc tgacgatggt gccggtcacg gccacggtgc ggtacttcaa aggcgtgaat
    26401 tccgccgtca gcgtggaggc gctgggcagg gcgccgccaa tgcccaggcc ggcgatgaag
    26461 cgcagcacgg tgatggcgtc gatgtttggg gcaaagccga tggcgatggt ggtggcgcca
    26521 aacagcgcca cgctggcggc gatcaccagc cggcgcccga tgcggtcggc catgtagccg
    26581 gcgaaggcgc tgcctatcgc catgccgacc aggccggtgg ccacggccgg ggcaaaggcg
    26641 ctgcgcgcta tgccccattc cttcatcatg acgggaatgg caaagcctat gagctgaccg
    26701 tcaaagccgt ccaggatgat ggacagtgcg gccatcagca ccaccatttt ctgcaggctg
    26761 gaaaacgggc cgtggtctag cgtgcggcct atgtctacgg ctgctgcgct gtgtgcgtgg
    26821 gacatggaga aatctccttt ttttatagcg ccgaacgctg atgcggcggc ttgtctcttt
    26881 ggggtggggg gtgaagcggg gcgcagcagc gcccgccacg cggcgcctgg gcgcgcgcgc
    26941 gggtggttgg gtaggggtag ggggcttgcc gcggcgtcag ccgcaggcca tcagtgcggg
    27001 cctacgcggc ccgagccgtc gtgcatccag cgggcgtggg tgggggcctg cttggtgcga
    27061 tcccactcgt tgagcatgtc ccacttcaca tcgtccagcg ccttgtccat ctgcggcgtg
    27121 gaggtgttgg ccgccagctc caggcggtgg cccgaggggt cgcgaaagta gatcgactgg
    27181 aagatggtgt ggttggtggg gccaatcacc tcgatgccgt cggcctccat cttggccttg
    27241 accttcatca tggtttcgac cgaatccacc ttcagcgcca ggtgctgcg
//
LOCUS       DAOMTQ010000020        27160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_166_length_27160_cov_24.970374, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000020 DAOMTQ010000000
VERSION     DAOMTQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27160
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_27160_cov_24.970374"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PK518_03585"
     CDS             <1..477
                     /locus_tag="PK518_03585"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069512.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRM48022.1"
                     /translation="ERIAFQGLPARICWLGLGERHIAGLAFNEMVKNGELKAPIVIGR
                     DHLDTGSVASPNRETEGMKDGTDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGYSQ
                     HSGMVIVADGTDAAAERLARVLVNDCGSGVMRHADAGYELAIATAKKQGLKLPMIK"
     gene            491..1417
                     /gene="hutG"
                     /locus_tag="PK518_03590"
     CDS             491..1417
                     /gene="hutG"
                     /locus_tag="PK518_03590"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRM48023.1"
                     /translation="MSAPFVWQGRIDTEEVGDSRRWHQCVQPFTPQSRSGVALIGFAV
                     DEGVRRNGGRPGAAQGPAAARKALANLPILGEPALWDAGDAVCEGGALDAAQTALAQR
                     VAQALAQGCTPLVLGGGHEVAWGTFQGVALARPGARILIVNLDAHFDLRQAREGNSGT
                     PFRQIQEHCAARGLPFDYRVLGISRFANTQALFDRADALQVRYVLDDALQTEAGVHAA
                     LADLAPDLQACDAVYLTLDTDVLPAAVAPGVSAPAPLGVPLWCVEAVVDAVLASGKLV
                     AADVAEFNPAFDRDGITAKVVARLAARIARVC"
     gene            1474..2223
                     /locus_tag="PK518_03595"
     CDS             1474..2223
                     /locus_tag="PK518_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM48024.1"
                     /translation="MTHSVSLTAVPTADRVLQVLAVLAQQGKALSAAELMEHTGLARS
                     TLYRQLARLKRWGFVLESDGYYAPGPLGLQLALGFDLASHLVRQARPAMVELAQQSQE
                     SVGLVVAVNDRAICLDMVESQQSLRCSFEKGRSVPLRAGASAKCLLAHLPEAARMGVL
                     DAQWGPGTPARQAAQDELDAIRRAGFAMSAAEVDPGVWGCSVPLFGATRQAVGALTLM
                     APILRAQGQEQVLIRMAVVAAARISRQLAIH"
     gene            2249..2827
                     /locus_tag="PK518_03600"
     CDS             2249..2827
                     /locus_tag="PK518_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HRM48025.1"
                     /translation="MHSFDLAAVPHTPWKNGGGHTRELACWPPGAGMEGFEWRVSVAT
                     IAAPGPFSAFAGVDRRIMLLDGDGMQLRARDGSLDHRLDRRWQPLAFAGEVAVDCTLL
                     GGTSTDFNLMLRRGRWRGEVQVVRDPRQPGSTPVGLCLVLAGRWSRGDDRLTAGQGLW
                     WSAAEAGPALVPQAGQGGDAPALAWVELRPII"
     gene            2893..4146
                     /gene="hutI"
                     /locus_tag="PK518_03605"
     CDS             2893..4146
                     /gene="hutI"
                     /locus_tag="PK518_03605"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRM48026.1"
                     /translation="MKHADSTTAPSADGVWEDLRLAPGLFRDDRPLAEHEAASLVVQG
                     GVLRWVGPSSALPLDYAGLARHAGGGLLATPGLVDCHTHLVYGGQRAGEFAMRLAGAS
                     YEEVAKAGGGIVSSVRATRAASEDELFAAALPRLSALLDEGVCAIEIKSGYGLALEHE
                     RKQLRVARRLGRALGVTVRTTFLGAHALPPEYAGRSQDYVDLVCSEMLPAMAAEGLVD
                     AVDVFCERIAFSLAETEQVFQAAQKLGIPVKLHAEQLSDMGGARLAARHGALSCDHIE
                     HLSQDGVDAMRAAGTVAVLLPGAYYTLRDTHLPPIEALRAAGVPMAVSTDHNPGTSPA
                     LSLLLMANMACTLFRLTVPEALAGITTHAARALGLSETHGLIAAGRPANFVLWNLREA
                     AELAYWFGHNPARTIVRQGRIVKGA"
     gene            4151..>4895
                     /locus_tag="PK518_03610"
     CDS             4151..>4895
                     /locus_tag="PK518_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM48027.1"
                     /translation="MHMIQTLARPEVPQLPAYNAGLSSEAVRARYGVSEIARLASNEN
                     PYGASPAVARALVDLATRVGCYPDANCSALRAAIGARTGVAPAAIVIGNGSEDILQML
                     CQAFLSAGERVLTQRPAFGLHEIYPRMMGAQVELLALTPRLGFDVDAWCAALARGPKL
                     AMLANPSNPVGCMFSAAEMQQLLAATPAHTLLVIDEAYYEYARLAPGYPDVLALLRGR
                     AMPWIVLRTFSKAWGLAGLRVGYGLASDAA"
     assembly_gap    4896..4995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4996..5421
                     /locus_tag="PK518_03615"
     CDS             <4996..5421
                     /locus_tag="PK518_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM48028.1"
                     /translation="AWGLAGLRVGYGLASDAALVQLLDRVRTPFNVNHAAQTAALAAW
                     GDEDFMRQGVTETVRLRGRLAARLAALPGVRVAPSATNFLFLDLGRPNAPVNEALLAQ
                     GLIVKPWKEAGFEHFIRVSIGTEAENERFARALEGILAL"
     gene            5454..6269
                     /gene="hutG"
                     /locus_tag="PK518_03620"
     CDS             5454..6269
                     /gene="hutG"
                     /locus_tag="PK518_03620"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HRM48029.1"
                     /translation="MPDTAPAPFLFHQGTAPLLISMPHAGTHVPPAIAARLTPEARQV
                     PDTDWHLPRLYEFAQALGASILVATHSRYVVDLNRPPDGTSLYPGQSVTGLCPVDTFD
                     DRPLYANPADLPDEAEVAARRAAYWAPYHAQLRAELERLRAAHGVAVLWDAHSIRSVL
                     PRFFEGRLPDLNLGTGDGISCDPALAQTLLGEARAATAYTAVLNGRFKGGHITRHYGA
                     PARNIHAVQLEMTQCSYMQEAPPFDYLPEVAAGVLPHLRRMVYAALAFARSRQ"
     gene            complement(6301..7176)
                     /gene="murI"
                     /locus_tag="PK518_03625"
     CDS             complement(6301..7176)
                     /gene="murI"
                     /locus_tag="PK518_03625"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRM48030.1"
                     /translation="MTSPPAARPIGVFDSGIGGLSVLQALRHELPHEDFIYLADSGNA
                     PYGDEKGEAFVQERTLAIARWLLQQHHIKALVVACNTATAAAVERLRASLPDLPAIGV
                     EPALKPAAARSQTHHVGVLATRGTVGSARFERLREQHSAHTRFAVQACDGLARAIEQS
                     TEEPLPALESAAKIRALCAQYTGALGPFGLKTGDIDTLVLGCTHYVFAKDVLRALVGP
                     DVQILDTGAAVARQTRRILTQAAALAPEQAADGEPAAPGRISLYTTGQLGALQAAAVR
                     WLELPAHCCAALPQG"
     gene            7349..8353
                     /locus_tag="PK518_03630"
     CDS             7349..8353
                     /locus_tag="PK518_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRM48031.1"
                     /translation="MHLDDSYHELGLAPGSSDAEVKAAWRRLSARWHPDRNDSPQALR
                     KMQRINWALKAIRQARAEPAAEAEDPAPGAEEFTVEHSVSLTLEDVVSGCVREVRGEV
                     VEDCVECEGSGLQSQATECSECNGAGHIRQHLWFTWVSSTVECAACQGQGKTHQACTA
                     CGASGKAPATKYRCRVQVAPGAHAGDLLNVTANVQGRQRQHRLALRVRVELQPHEFFT
                     AEADGTLRCELPVDGFAWMANRWIEVPTPRGLQQMKLRRGSLSYRIKNAGLPWTNTGE
                     AADCIVTVAPMFPQEFSPEQEAAIDRLVSSNSGAAGTSAGDQMAAWTRLVDSWQDRRR
                     "
     gene            complement(8614..9240)
                     /locus_tag="PK518_03635"
     CDS             complement(8614..9240)
                     /locus_tag="PK518_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HRM48032.1"
                     /translation="MTTPPADALPFSQACENNQAPILAVLQQAFADRRQVLEVGSGTG
                     QHGVYFAPRLPHLVWQTSDLAENHPGIRAWQAAHPAPNLRAPLLLDLAGSAWPDGAFD
                     AVFSANTTHIVAWPLVQRLFALAGAHLPAGGALALYGPFNYGGSFTSDSNRAFDAWLR
                     QRDAASGIRHFEDIVALGVEHDLALEADNAMPANNRLLVLRRAAPAYA"
     gene            complement(9277..10083)
                     /locus_tag="PK518_03640"
     CDS             complement(9277..10083)
                     /locus_tag="PK518_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HRM48033.1"
                     /translation="MTARNPFKTALAERRPQIGLWLSMADPYQAEAAATCGYDWLLID
                     GEHAPNDLRSTLAALQAVNAYPAHPVVRVVEGSQALIKQMLDIGAKTLLVPMVDTASQ
                     ARAIAAATRYPPQGVRGVGSATGRSSLWSSRSDYLNVANDEVCLLLQAETATALQNLE
                     AICAVDGVHGIFIGPADLAASMGHRGNPGHPEVQAAIAGAMKTIIASGKAAGTLTSDP
                     ALAQRYLDLGCTFVAVGVDVLLFVNAARRLRGQFAGAGAAPAASGPSAAY"
     gene            complement(10097..11566)
                     /locus_tag="PK518_03645"
     CDS             complement(10097..11566)
                     /locus_tag="PK518_03645"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRM48034.1"
                     /translation="MPLTLTRPDLIRSANFIAGQWSATPAARLAVTDPATGATITEVP
                     DSGALEARAALDAAHAAFPAWRKVPAKQRAAIIKRWNDLVLAHQDDLGKLISREQGKP
                     LAEGKGEVAYAASYIEWFGEEATRMNGEIIPAPLPGRRMFALREPVGVVAAITPWNFP
                     AAMIARKIAPALAAGCTVVCKPAEDTPLTSLALVLLAQEAGVPAGVLNIVTASREKTP
                     EVVDVWLDDARVRKITFTGSTPVGKHLARRSADTLKKLSLELGGNAPFIVFDDADVDA
                     AVDGLMAAKFRNGGQTCVCPNRVFVQRGVFDTFAQKLAARVAALKVGPASDPASQIGP
                     MINDRAVEKIERHVQDAVAKGAKVLAGGKRLPELGATYYAPTVLSGADARMACACEET
                     FGPVAPLTMFDSEDEVLQAANDTPFGLAAYFYSQDVRRIWRVADALESGIVGINEGAI
                     SAEAAPFGGVKESGYGREGSTHGLDDYLHTKYICQGQLD"
     gene            complement(11626..13167)
                     /locus_tag="PK518_03650"
     CDS             complement(11626..13167)
                     /locus_tag="PK518_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HRM48035.1"
                     /translation="MATIDSPLLHLHPDDNVFVAKSALALGQTISELGVRTRAQVPAG
                     HKIAARHIAAGEQVKKFDTVIGVATRDLAPGDYVHSHNLKIVDYYRDPAFGADVRPVD
                     YLPEEQRATFQGYVRADGRVGTRNFIGILSSVNCSATVIRQIAAHFTPERLAAFPHVD
                     GVVAFAQTSGCGMSSPSEHFDVLRRTLAGYARHANLAGALIVGLGCERNQVGALVESQ
                     GLKDDPANGQLLRTLVMQEVGGTRVTIEAGIRAIEEMLPIANQLRRQTVSASHIKIGL
                     ECGGSDGFSGITANPALGAAMDILVRHGGTAILSETPEIHGVEFMLTRRAISPAVGQK
                     LLDRLAWWERYAAGQNAQFNGVVGHGNQAGGLANIFEKSLGSAMKGGTTPLRAVYEYA
                     EPITEHGFVFMDSPGYDPVAVTGQIASGANLICFTTGRGSMFGSKPAPTMKLASNTPM
                     YQRLSEDMDINCGLIVDGECSVAEMGQRIFEQILRHASGEPTKSEQLGLGDNEFVPWH
                     LGIVS"
     gene            complement(13179..14168)
                     /locus_tag="PK518_03655"
     CDS             complement(13179..14168)
                     /locus_tag="PK518_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48036.1"
                     /translation="MTIERNTRRQLILGALGATALASSPARLLAQGWPERSITFICPW
                     PAGGTADQSMRALCTVAARVLGQAIAVENRVGASGMIGAKALASARPDGYTIGQIPIS
                     VTRFSQLGTLAADPRKDYSYIARTSGQTFGIAVLPGSQFKTLKDFVDFAKANPGKVTY
                     AHAGVGGATHVGMEEFAMAAGIQLNHVPYKGGAEALQGVLGGHVDVLADSSSWAPHVE
                     AGKLRLLATWGEQRTQRFRDTPTLKELGYNVVVDAPNGIGAPKGVDPAILARLREAFK
                     QAVASAEFKAVTDKLDAPLLYLDGPDYEQYVNTVYQKETVLIDKLKLRELMRS"
     gene            14294..15232
                     /locus_tag="PK518_03660"
     CDS             14294..15232
                     /locus_tag="PK518_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM48037.1"
                     /translation="MALIDRVLRSNLKLRHLQMLVALDQFRHLGRTAEFLSLTQPAVS
                     KTLAEIESMFGLPLFERSTRGTEPTAAGASVVRFARLVLAGYERTREEIAAEASGAMG
                     RTSVGAMVAATPVLLAQAVQRLKQHSSRTTVLIDEGDLTRLLPKLRLGELDLFVGRLE
                     PAYASPDLETEALYEDPMAAVVCTGHPLADVRAVQWADIAAQPCVLPPPWASLRVKLE
                     QQFFAHELHPPADIIESASFLVQLTFMQQRGAVAFMARSVARHHAQLGMVALLDLPVR
                     IALPPVGLITLRGQRMTPVTAALVECLRDEARLLAQ"
     gene            complement(15234..16265)
                     /locus_tag="PK518_03665"
     CDS             complement(15234..16265)
                     /locus_tag="PK518_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRM48038.1"
                     /translation="MRRHAVWRPLTAIGLAGLAACSNTTPSQPTITPQATATAPPAPI
                     KIGIALGGGAAKGFAHVGVIKMLEANGLAPAVVAGTSAGSVVGALYASGMNAFELQDK
                     AVALDESKVRDLQLSSGGLVQGQKLEDYVNEQVRRKSLEQLAKPFVAVATRLEDGERT
                     LFARGNTGQAVRASSSVPGVFQPVSIGKYHFVDGGVVSPVPVDAARQLGADMVIAVDI
                     SNKARGKTPEHMLGALNQSIAIMGQKLGQAELARADVVIRPQVLDMGPVDFNQRTQAI
                     AEGEKAALAAMAQIRERIAQLQAERAQATRLAQQKASEAARQACLDQRSRLDKLASVA
                     GLGDSCAPR"
     gene            complement(16459..17808)
                     /gene="gorA"
                     /locus_tag="PK518_03670"
     CDS             complement(16459..17808)
                     /gene="gorA"
                     /locus_tag="PK518_03670"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HRM48039.1"
                     /translation="MKHYDYIVIGGGSGGIASANRAAMYGRRCALIEAHEIGGTCVNV
                     GCVPKKVMWHAAQIAEAIHRYGPDYGYDTRVNRFDWQRLIESRSAYIARIHESYDSGL
                     QRNKVDVIRGFGRFVDARTIEVEGRRLSADHILIATGTRPTWPAIPGAELGLDSDGFF
                     ALPALPARTAVVGAGYIAVELAGVLHALGSQTHLFLRRASALRHFDPMLGETLMQVME
                     AEGPTLHRHATPQSVTRNADGSLCLRLEDGRSHNVDCIVWAIGRRPDTSGLGLEAAGV
                     ALDEGGYIQVDKFQNTNVGGIYAVGDITGRIELTPVAVAAGRRLSERLFNNRPDEHLD
                     YENIPSVVFSHPPIGTVGLTEPQARERFGDAQVKVYQAAFTAMYTAVTRHRQSARMKL
                     VCAGPEERIVGIHGIGHGMDEILQGFAVALKMGATKRDLDDTVAIHPTAAEEFVTMR"
     gene            complement(17922..19010)
                     /locus_tag="PK518_03675"
     CDS             complement(17922..19010)
                     /locus_tag="PK518_03675"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HRM48040.1"
                     /translation="MQTTYKIAVLPGDGIGKEVMPEGLRAVEAAAQRFGLSLEFHHFD
                     WAHCDYYAAHGKMMPDDWKAQLQGMDAIYYGAVGWPATVPDHVSLWGSLLKFRREFDQ
                     YINLRPVRLFEGVPCPLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTEREIVIQES
                     VYSRHGANRLLKFAFELAQSRAKKHVTLATKSNGIAISMPWWDQRAEDMAKHYPEVAL
                     DKQHIDILTARFVLQPGRFDVVAATNLFGDILSDLGPATTGTIGLAPSANLNPERSFP
                     SLFEPVHGSAPDIYGKNIANPIAMIWSGALMLDFLTQGQGAGRAAHDAIVAAIEQVIK
                     SGPRTPDLGGQASTQQVGKAIAATITKG"
     gene            19100..20002
                     /locus_tag="PK518_03680"
     CDS             19100..20002
                     /locus_tag="PK518_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM48041.1"
                     /translation="MQKIPPTDDLRVFCTVVRKAGFAAAAQELGASPAYVSKRIRLLE
                     QELGVPLLHRTTRRVVVTDQGERVFHWAQRILDDMDQLLQEVAVTRREPRGLLRVSSS
                     FGFGRNVVASALSRLVRQHPGLQVRLEVFDRLVDVAAEGFDLDVRVGDEIAPHLIARR
                     LADNHRVLCAAPSYLARRGTPRTPAELPAHDCLVIKERDHPFGVWRLRAGAQEQSLKV
                     TGPLSANNGEMVVQWAVDGCGIALRSRWDVQQHLHAGRLVQLLPEWRQEANVWAVYPT
                     RLQLSAKVRVCVEFLQAYFRQEWG"
     gene            complement(20024..20935)
                     /locus_tag="PK518_03685"
     CDS             complement(20024..20935)
                     /locus_tag="PK518_03685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM48042.1"
                     /translation="MSKPFISLCPEITRAHALHLMDWLEDESVTRYLSDSRHVSRFIE
                     QVIGRVQLPILTHLFNQGGRFFMAYDRHDQPVGFVRLVKNGPDCEIVLLIGSRDNWGR
                     KLGASALREGMKLAFFDMRAERLIAKIHADNTRSLKAFQRCGFLPDGATPTLKSYAMT
                     SAHYLQHLRAGTMGDSCQIHITEVDQARLRDLVALELDAHVVDLEHEIERAIVVDARQ
                     VARNVVTMNSRALLHLDDEKVEVALVYPQDADDSAGKLSVYSDIGTAILGYKEGDAFD
                     WRIPNRTRRISIEKVLYQPEAAGDFDL"
     gene            complement(21165..21626)
                     /locus_tag="PK518_03690"
     CDS             complement(21165..21626)
                     /locus_tag="PK518_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRM48043.1"
                     /translation="MSLKDQITEDMKTAMRAKDSERLGTIRLLQAAMKQKEVDERITL
                     DDAAIVAIVDKLIKQRKDSITAFEGAGRQDLADKEKAEMAVLQAYLPARMSEAEILDA
                     VKAIVAELGAELGRQAGPGDMGKVMGVVKTRLAGKAEMGQVSSAVKAALAG"
     gene            complement(21716..21928)
                     /gene="rpsU"
                     /locus_tag="PK518_03695"
     CDS             complement(21716..21928)
                     /gene="rpsU"
                     /locus_tag="PK518_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRM48044.1"
                     /translation="MTTIRVKENEPFDVALRRFKRTIEKLGLLTDLRAREFYEKPTAE
                     RKRKKAAAVKRHYKRVRSMQLPKKLY"
     gene            22224..23702
                     /gene="sufB"
                     /locus_tag="PK518_03700"
     CDS             22224..23702
                     /gene="sufB"
                     /locus_tag="PK518_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRM48045.1"
                     /translation="MSTPSENLDTPVEQAPALEQALARPYQHGFVTDIESVSLPPGLD
                     EDTIRSISRIKREPQFLLDWRLAAFKRWQAMELPDWARLNIAPVDFQALSYYSAPKSM
                     KDRPKSLDEVDPKLLETYEKLGVPLHERAALAGVAVDAVFDSVSVGTTFRKQLADVGV
                     IFCSFSHAVQNHPELVEKYLGTVVPQGDNFYAALNSAVFSDGSFVYIPKGVRCPMELS
                     SYFRINAQNTGQFERTLIIAEEGSQVSYLEGCTAPQRDENQLHAAVVELVAHDDAFIK
                     YSTVQNWYPGDENGKGGIYNFVTKRGLAAGKRSRIAWTQIETGSAITWKYPSVVLRGD
                     ESNGEFHSIAVSNHWQQADTGTKMIHLGKNTTSRIVSKGISAGNAQNSYRGLVRINPG
                     AAGARNHTQCDSLLIGPHCGAHTYPYVDTARSDAVVEHEATTTRISEERLFYCEQRGI
                     DAQDATALIVGGFCQAVLEELPMEFALEAKALLAVSLEGAVG"
     gene            23774..24544
                     /gene="sufC"
                     /locus_tag="PK518_03705"
     CDS             23774..24544
                     /gene="sufC"
                     /locus_tag="PK518_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRM48046.1"
                     /translation="MTHTPPLLQVRDLRVNIGERTVLHSVSLDVQPGQLVVLMGANGS
                     GKSTLGMALAGHPHYQVQGGQALLAGQDLLALPAHERARAGLFLSFQSPPDVPGVKNN
                     LFIRTALNAQHEARGQEPLDAFDFLSSAKKAAKDLGLPEAMLGRPVNEGFSGGERKRN
                     ELLQLSLLKPRLALLDEIDSGMDVDGVRAVVALVQKLRAEGTAFIVISHYLQMIESLE
                     PDAVLRLHQGCIAETGDLELAREIARTGFARAPQAVEA"
     gene            24544..25803
                     /locus_tag="PK518_03710"
     CDS             24544..25803
                     /locus_tag="PK518_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM48047.1"
                     /translation="MDSARADILAARQHLQQQGWIARKSEAFHHLPPPALDQWLGAQG
                     ESGCEIPALSGAGWTLHPLHRPDGNHGASGIHARWLDAHDPAERSELFAGLPRPGNTG
                     VDGEAAPFAWAHRALCRLGLRLRISATPGKEREPVWLELRHQPRARAEAPLLVLEVDP
                     GVQCVLIESHEREVGSCQHAVAQNLHAHIHLGAGAQLQHLRIAAPVADDSVAHHLHIN
                     VAAGAQYAQALVATSAGYHLQRSLVQLQGKGAQAHSAALLLAGAHKIDHQSYTRMQAA
                     HSASTVETLTLAQGKAHAVANAHTTIAPGADEANVRQRLAGVPLSGNPRLILRPHLEI
                     LHDNVQAAHGATWGALPEDALFYARQRGLDEASARSLIIEGMARALLQRCLGQDDDGL
                     LAQWLGGGWLARAIAVQLQADMEERHG"
     gene            25919..>27160
                     /locus_tag="PK518_03715"
     CDS             25919..>27160
                     /locus_tag="PK518_03715"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004536388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HRM48048.1"
                     /translation="MRGQPGEQEQQDLSHLRAQFPILAQSIHGHPLAYLDNAATTQTP
                     LAVQQAMQQFEQRDRANIHRGVHTLSQRATDAFEAARATLKAFVGAGPAHELVFTSGT
                     TEALNLVAQGLWAAGPGKAWLKPGDEIIVSGLEHHANIVPWQQAARMSGATLSVLRPD
                     AEGRIHTQDLQRLLGARTRVLAMTAGANATGERPPYEEMLALARQAGALTVLDAAQAV
                     GHAMPALSALDCDFMAFSGHKMYGPMGTGALVGKREVLQLLYPLRLGGDMVDWVSYEA
                     AGYAQLPARLEGGTPNVAGAVGLAAAARFIDEVGRTAIDQHLHALRAQALAGLQAIDG
                     ISVLAPHATQASLVSFVSHDVHPHDIGTLLDERGIAVRTGHHCAQPLLDHLGLGPTTR
                     ASFALYNTSDEVDRFVAAVAHA"
BASE COUNT         4085 a         9066 c         9463 g         4446 t
ORIGIN      
        1 gagcgcatcg ccttccaggg cctgcccgcg cgcatctgct ggctgggcct gggcgagcgc
       61 cacatcgcgg gcctggcctt caacgagatg gtgaagaacg gcgagctgaa ggcccccatc
      121 gtcatcggcc gcgaccacct ggacaccggc agcgtggcca gccccaaccg cgagaccgaa
      181 ggcatgaagg atggcaccga cgccgtgagc gactggccgc tgctcaacgc cctgctcaac
      241 accgccggcg gcgccacctg ggtcagcctg caccacggcg gcggcgtggg catgggctac
      301 agccagcact cgggcatggt catcgtggcc gacggcacgg acgccgcggc cgagcgcctg
      361 gcgcgcgtgc tggtcaatga ctgcggctcg ggcgtgatgc gccatgccga tgcaggctac
      421 gagctggcca ttgcgacggc gaagaagcag ggcctgaaac ttcccatgat caagtaaagc
      481 aggggacacc atgagcgcac cctttgtctg gcaaggccgc atcgacaccg aagaagtggg
      541 cgacagccgc cgttggcacc agtgcgtgca gccttttaca ccccagagcc gctcaggcgt
      601 ggccctgatc ggctttgccg tggacgaggg cgtgcgccgc aacggcggcc gcccgggcgc
      661 tgcgcaagga ccagcggcgg cgcgcaaggc gctggccaac ctgcccattc tgggcgagcc
      721 ggcgctctgg gatgcgggtg atgcggtctg cgaaggcggt gcgctcgatg cggcgcaaac
      781 cgccctggcg cagcgggtgg cgcaggccct ggcgcagggc tgcacgcccc tggtgctggg
      841 cggtgggcac gaggtggcct ggggcacctt ccagggcgtg gcgctggcac ggcccggcgc
      901 gcgcatcctc atcgtcaacc tggacgcgca tttcgacctg cgccaggcgc gcgagggcaa
      961 ttcgggcacg ccgttccgcc agatccagga acactgcgcc gcgcggggcc tgccgtttga
     1021 ctaccgcgtg ctgggcatca gccgctttgc caacacccag gccttgttcg accgtgccga
     1081 tgccttgcag gtgcgttatg tgctggacga cgccttgcag accgaagccg gcgtacacgc
     1141 cgcgctggcc gatctggcgc ccgacctgca ggcctgcgac gccgtgtacc tgaccctgga
     1201 taccgacgtg ttgcccgccg ccgtggcccc cggcgtgtcg gcgcctgcgc cgctgggcgt
     1261 gccgctgtgg tgcgtggagg ccgtggtgga tgcggtgctc gccagcggca agctggtggc
     1321 ggccgatgtg gccgaattca accccgcctt cgaccgcgac ggcatcaccg ccaaagtggt
     1381 cgcccgcttg gcggcgcgca ttgccagggt gtgctgaaca ggcgtattga agaggggccc
     1441 gcattcccat tctttgactt tggtactgcc gccatgaccc acagtgtttc cctcaccgcg
     1501 gtgcccacgg ccgaccgtgt gctgcaggtg ctggccgtgc tggcccagca gggcaaggcc
     1561 ttgtcggccg ccgagctcat ggagcacacg ggcctggcgc gcagcacgct gtaccgccag
     1621 ctggcgcggc tcaagcgctg gggcttcgtg ctcgaaagcg acggctatta cgcgcccggg
     1681 ccgctcggcc tgcagctcgc gctggggttc gacctggcct cgcacctggt gcgccaggcg
     1741 cgccccgcca tggtggagct ggcgcagcag tcgcaggaaa gcgtgggtct ggtcgtggcc
     1801 gtgaacgacc gggccatctg cctggacatg gtggagagcc agcagtcgct gcgctgctcg
     1861 ttcgagaagg ggcgcagcgt gcctctgcgt gcgggcgcct cggccaaatg cctgctggcc
     1921 cacctgcccg aggcggcacg catgggcgtg ctcgacgccc agtggggccc gggcacgccc
     1981 gcacgccagg ccgcgcagga tgagctcgat gccattcgcc gcgccggctt tgccatgagc
     2041 gctgccgagg tggaccccgg cgtctggggc tgcagcgtgc cgctgttcgg tgccacgcgc
     2101 caggccgtgg gcgccctcac gctgatggcg ccgatcctgc gtgcccaggg gcaggagcag
     2161 gtgctgatcc gcatggccgt cgtcgctgcg gcgcgcatat cgcgccagtt ggcaatccac
     2221 tgaatcctct gcaagccctg atctgcccat gcactccttc gacctcgcgg ccgtgccgca
     2281 caccccctgg aagaacggcg gcggccacac gcgcgagctg gcctgctggc cgcctggcgc
     2341 gggcatggag ggcttcgagt ggcgcgtgag cgtggccacc atcgccgcgc ccgggccatt
     2401 ctcggccttc gccggcgtgg atcgccgcat catgctgctc gatggcgacg gcatgcagct
     2461 gcgcgcccgg gatggcagcc tggaccaccg gctggacagg cgctggcagc cgctggcctt
     2521 tgcgggcgag gtggccgtgg actgcaccct gctgggcggc acctccaccg acttcaacct
     2581 catgctgcgc cggggccgct ggcgcggcga ggtgcaggtg gtgcgcgacc cacgccagcc
     2641 cggcagcacg cccgtgggcc tgtgcctggt gctcgcgggc cgctggagtc ggggtgacga
     2701 tcggctgacc gccggccagg gcctgtggtg gagcgccgcc gaggccggcc ctgcgctcgt
     2761 gccgcaggcg gggcagggcg gcgatgcgcc cgccctggcc tgggtggaat tgcgacctat
     2821 tatttgatga aatatgccac ttgcgcttga tggacaagcg ccaatagcta taactaatgt
     2881 agtaaatgag ccatgaaaca cgctgattcc accactgccc ccagcgccga cggcgtctgg
     2941 gaagacctgc gcctcgcccc cgggctgttc cgtgacgaca ggcccttggc cgagcatgag
     3001 gccgccagcc tggtggtgca gggcggggtg ctgcgctggg tgggaccgtc ctcggcgctg
     3061 ccccttgact acgcaggact ggcgcgccat gccggcggcg gcctgctggc cacgcccggc
     3121 ctggtggatt gccacacgca cctggtttat ggcggccagc gtgcggggga gttcgccatg
     3181 cgcctggccg gcgccagcta cgaggaggtt gccaaggccg gcggtggcat cgtctccagc
     3241 gtgcgcgcca cgcgcgccgc cagtgaggac gagctgttcg ccgccgcgct gccgcgcctc
     3301 tcggcactgc tggacgaggg cgtgtgcgcc atcgagatca agtccggcta tggcctggcc
     3361 ctggagcatg agcgtaagca gctgcgcgtg gcgcgccgcc tgggccgggc gctgggcgtg
     3421 acggtgcgca ccaccttcct cggcgcgcac gcgctgccgc ccgaatatgc ggggcggagt
     3481 caggactatg tcgatctggt atgcagcgag atgctgcccg ctatggcggc cgagggcctg
     3541 gtggatgccg tggatgtgtt ctgcgagcgc atcgcctttt cgctggcgga aaccgagcag
     3601 gtgttccagg ccgcgcaaaa gcttggcatc ccggtcaagc tgcatgccga gcagctctcg
     3661 gacatgggcg gcgcgcggct cgccgcacgc catggcgccc tgtcctgcga ccacatcgag
     3721 cacctgtcgc aggacggagt agacgccatg cgcgccgccg gcaccgtggc cgtgctgctg
     3781 cccggcgcct actacacgct gcgcgacacg catctgccgc ccattgaggc gctgcgcgcg
     3841 gccggcgtgc ccatggccgt atccaccgac cacaaccccg gcacctcgcc cgcattgagc
     3901 ctgctgctca tggccaatat ggcctgcacc ctgttccgcc tcacggtgcc cgaggcgctc
     3961 gcgggcatca ccacgcacgc ggcgcgcgcg ctgggcctgt cggaaacgca cggcctgatc
     4021 gcggcgggcc ggcccgccaa ctttgtgctg tggaacctgc gcgaagccgc cgagttggcc
     4081 tattggttcg gccacaaccc ggcgcgtacc atcgtgcgcc agggccgcat cgtcaaggga
     4141 gcgtgaagcc atgcacatga tccagaccct ggcgcggccc gaagtgccgc aactgcccgc
     4201 ctacaacgcc ggcctgtcgt ccgaggccgt gcgtgcgcgc tacggcgtga gcgagatcgc
     4261 gcgcctggca agcaacgaga acccctacgg cgccagcccc gccgtggcgc gcgccctggt
     4321 cgacctggcc acgcgcgtgg gctgctaccc cgacgcgaac tgcagcgcgc tgcgcgcggc
     4381 gattggcgcg cgcacgggcg tggcgccggc ggccatcgtc atcggcaacg gctccgagga
     4441 catcctgcag atgctgtgcc aggccttcct gtccgcgggc gagcgcgtgc tcacgcagcg
     4501 cccggccttt ggcctgcatg agatctaccc gcgcatgatg ggcgcccagg tcgagctgct
     4561 ggcgctcacg ccgcggctgg gctttgacgt ggatgcctgg tgcgcggcgc tggcgcgcgg
     4621 ccccaagctc gccatgctgg ccaatccctc caaccccgtg ggctgcatgt tcagtgcggc
     4681 cgagatgcag caactgctcg ccgccacgcc cgcgcacacc ctgctggtga tcgacgaggc
     4741 ctattacgaa tatgcccgcc tggcccccgg ctaccccgat gtactggccc tgctgcgcgg
     4801 ccgcgccatg ccctggatcg tgctgcgcac cttctccaag gcctggggcc tggccggcct
     4861 gcgcgtgggc tacggcctgg ccagcgatgc ggcgcnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnngcctg gggcctggcc ggcctgcgcg tgggctacgg cctggccagc
     5041 gatgcggcgc tggtgcaact gctcgaccgc gtgcgcacgc ccttcaacgt gaaccatgcg
     5101 gcgcagacgg cggcgctggc cgcctggggc gacgaagact tcatgcgcca gggcgtgacc
     5161 gagaccgtgc gcctgcgcgg gcggctggcg gcgcggctcg ccgccctgcc gggcgtgcgc
     5221 gtggcgcctt cggccaccaa cttcctgttc ctcgacctgg gccgccccaa tgcgcccgtg
     5281 aacgaggcgc tgctggccca gggactgatc gtcaagccct ggaaggaggc gggcttcgag
     5341 cacttcatcc gcgtctccat aggcaccgag gccgagaacg agcgcttcgc gcgggcgctt
     5401 gaaggcatcc tggcgcttta ggacctgttc acaaccttcc agggactcac acgatgccgg
     5461 acaccgcccc cgcgccgttc ctctttcacc agggcacggc gccgctgctc atctccatgc
     5521 cccatgcggg cacccatgtg ccgccggcca tcgccgcgcg cctgacgccc gaggcgcgcc
     5581 aggtgcccga caccgactgg cacctaccgc gcctgtacga gtttgcccag gcgctgggcg
     5641 cctccatcct cgtggccacg cattcgcgct acgtggtgga cctgaaccgc ccgcccgacg
     5701 gcaccagcct ctaccccggc cagagcgtga ccggcctgtg ccccgtcgat accttcgacg
     5761 accggccgct gtacgccaat cccgccgatc tgcccgatga ggccgaggtc gccgcgcgcc
     5821 gcgccgccta ctgggcgccc taccacgccc agctgcgcgc cgagttggag cgcctgcgcg
     5881 ccgcgcacgg cgtggccgtg ctctgggacg cgcactccat ccgctcggtg ctgccgcgtt
     5941 tcttcgaggg caggctgccc gacctgaacc tgggcacggg cgacggcatc agctgcgacc
     6001 cggccctggc ccagaccctg ctgggcgagg cccgcgccgc cacggcttac accgccgtgc
     6061 tcaacggccg cttcaagggt ggccacatca cgcgccacta tggcgcgccc gcgcgcaaca
     6121 tccacgccgt gcagctggag atgacgcagt gcagctacat gcaggaggcg ccgccgttcg
     6181 actacctgcc cgaggtggcg gccggcgtgc tgccgcacct gcgccgcatg gtctatgcgg
     6241 ccttggcctt cgcccggtcg cggcagtagc ggcagtagcg gtagtggggc tggttcggat
     6301 tcagccctgg ggcagcgccg cgcagcagtg ggccggcagc tccagccagc gcacggccgc
     6361 ggcctgcagt gcgcccagct ggcccgtggt gtagaggctg atgcgtcccg gcgcggccgg
     6421 ctcgccgtcc gccgcctgct cgggcgccag cgcggcggcc tgggtcagga tgcggcgcgt
     6481 ctggcgcgcc acggcggcgc cggtgtccag gatctgtacg tccgggccca ccagcgcgcg
     6541 caacacatct ttggcgaaca catagtgcgt gcagcccagc accagcgtgt cgatgtcgcc
     6601 ggttttcagg ccaaacgggc ctaaagcgcc cgtgtattgt gcgcaaagcg ctcttatttt
     6661 tgctgcagat tcgagcgccg gcaggggctc ctcggtgctc tgctctatgg cccgcgccag
     6721 gccgtcgcag gcctgcacgg caaagcgcgt gtgcgccgaa tgctgctcgc gcaggcgctc
     6781 aaagcgcgcg ctgccaaccg tgccgcgcgt ggccagcaca cccacatggt gcgtctggct
     6841 gcgcgcggcg gcgggtttca gcgccggctc cacgccaatc gccggcagat ccggcaggct
     6901 ggcgcgcagc cgctccaccg ccgccgccgt ggccgtgttg caggccacca ccagggcctt
     6961 gatgtgatgc tgctgcagca gccaccgggc aatggccagc gtgcgctctt gcacaaaggc
     7021 ctcgcccttc tcgtcgccat agggcgcgtt gccgctgtcg gccaggtaga tgaagtcctc
     7081 gtgcggcagc tcgtggcgca gggcctgcag cacgctcagg ccgcctatgc cgctgtcgaa
     7141 tacgcctatg ggacgggcgg cggggggcga tgtcatggaa ggcgtgggga gggttggtgt
     7201 agtcgggtgc gcctgccgtc gcggcggcgg cgcgggtggc aagtatgcct gggtgggtgc
     7261 cagcccagtg catccagcgt gagaggcgcg acggcaagct gtgcctgcta ccatgctgca
     7321 tcgcagcaaa tcttgcgcaa cggagatcat gcacctcgac gactcttacc acgaacttgg
     7381 cctggcaccg ggttccagcg atgccgaggt gaaggcggcg tggcgccgcc tgtcagcgcg
     7441 ctggcacccg gatcgcaacg acagcccgca ggccctgcga aagatgcagc gtatcaattg
     7501 ggcactgaaa gcgatccgcc aggcccgggc tgagccggcg gccgaagccg aggatcccgc
     7561 ccctggtgcg gaggaattca ccgtcgagca ctccgtgagc ctgacgcttg aggatgtggt
     7621 gagcggctgc gtccgggaag tgcggggcga ggtggtcgaa gactgcgtcg aatgcgaagg
     7681 cagtggcctg caatcgcagg cgaccgagtg cagcgaatgc aatggcgccg gccacatccg
     7741 gcagcacctc tggttcacct gggtatcgtc caccgtcgag tgcgccgcct gccaggggca
     7801 ggggaagacg caccaagcct gcaccgcctg cggggccagc gggaaggcgc cggcgaccaa
     7861 ataccgctgc cgggtgcagg tcgcgccggg tgcgcatgcg ggcgacctgc tgaatgtcac
     7921 ggccaacgtg caaggcaggc agcgtcagca caggctggcg ctgcgcgtgc gcgtggaact
     7981 gcagccgcac gagttcttca cggccgaagc cgacggaacc ttgaggtgcg agctacccgt
     8041 cgacggtttt gcatggatgg ccaatcgctg gatcgaggtt ccaacccctc gcggcctgca
     8101 acagatgaag ctgcgacgag gctccctgag ctatcgcatc aaaaatgcgg gcctgccttg
     8161 gacgaacact ggagaggctg cggactgcat cgtcaccgtc gcgccgatgt ttccgcaaga
     8221 attcagcccg gagcaggagg cagcgatcga tcggctggtg agtagcaact ctggggcggc
     8281 aggtacatcg gccggcgatc aaatggcggc atggactcgg ttggtggaca gttggcagga
     8341 tcgacgcagg tgagaccccc tgatggccat cagcatgatg gcccgctttt tgtcggcaga
     8401 acccagcctc gggtgtaacc gtgagcccca ttgccggtgg ctgcctcgcc ggatgcggtt
     8461 gatcgcttca cttgcacagg cacttcagcc ggcactgccg ctgggaatac ctgtttgctt
     8521 tcaaaaagat agctcttcgc gcttatgggg tgggcgaata atgtcaataa catccaaacc
     8581 gcatgccacg gctgcgcagc tgcggaagcc agcctatgcg taggcggggg ccgcgcggcg
     8641 cagcaccagc agccggttgt tggccggcat ggcgttgtcc gcctccagcg ccaggtcatg
     8701 ctctacgccc agcgccacga tgtcctcgaa gtggcgtatg ccgcttgccg catcgcgctg
     8761 gcgcagccag gcgtcaaacg cgcggttgct gtcgctggtg aagctgccgc cgtagttgaa
     8821 cgggccgtac agcgccagcg cgccgcctgc gggcaggtgg gcgccggcca aagcgaacaa
     8881 ccgctgcacc agcggccagg cgacgatgtg cgtggtgttg gcgctgaaga cggcgtcgaa
     8941 ggcgccgtcg ggccaggcgc tgcctgccag gtccagcagc agcggcgcgc gcaggttggg
     9001 cgccgggtgt gcggcctgcc aggcgcgtat gccggggtgg ttctcggcca ggtcgctggt
     9061 ctgccagacg aggtgcggca ggcgcggcgc aaagtacacg ccgtgctggc ccgtgcccga
     9121 gcccacctcc agcacctggc gccggtcggc aaaggcctgc tgcagcacgg cgagtatggg
     9181 ggcctggttg ttctcgcagg cctgcgaaaa gggaagggcg tcggcgggcg gggtggtcat
     9241 gccgcgcatg atccaagatt tgccatggcc ccgcgttcaa taggccgcgc tcgggccgct
     9301 tgccgcaggg gctgcgcccg cgccggcaaa ctggccgcgc aggcggcgcg ccgcgttcac
     9361 gaacagcagc acgtccacgc ccacggcgac aaaggtgcag cccaggtcga ggtagcgctg
     9421 ggccagcgcc gggtcggatg tcagcgtgcc ggccgccttg ccgctggcga tgatggtctt
     9481 catcgcgccc gctatggcgg cctgcacctc ggggtggccg gggttgccgc ggtggcccat
     9541 ggatgcggcc aggtcggccg ggcctatgaa gatgccgtgc acgccgtcca cggcgcagat
     9601 ggcttcgagg ttctgcaggg ccgtggcggt ctcggcctgc agcagcaggc agacttcgtc
     9661 attggccacg ttcagatagt cgctgcggct gctccacagc gacgagcgcc ccgtggcact
     9721 gccgacgccg cgcacgccct gcggcgggta gcgcgtggcg gcggcgatgg cgcgggcttg
     9781 gcttgccgtg tccaccatgg gcaccagcag ggtcttggcg cctatgtcca gcatctgctt
     9841 gatgagggcc tggctgccct cgaccacgcg caccaccgga tgcgccgggt aggcgttcac
     9901 ggcctgcagg gcggccaagg tgctgcgcag gtcgttgggc gcgtgctcgc cgtcgatcag
     9961 cagccagtcg tagccgcagg tggcggcggc ctcggcctgg tacgggtcgg ccatggacag
    10021 ccacaggccg atctgcgggc ggcgttcggc cagggcggtc ttgaaggggt tgcgggcggt
    10081 catggggcgt ccttgttcag tccaactggc cttggcagat gtacttggtg tgcaggtaat
    10141 cgtccagccc gtgggtggag ccctcgcggc cgtagcccga ttccttcacg ccgccaaagg
    10201 gcgcggcctc ggccgagatg gcgccctcgt tgatgccgac gatgccggat tcgagcgcgt
    10261 cggccacgcg ccagatgcgg cgcacgtcct ggctgtagaa gtaggcggcc aggccaaacg
    10321 gcgtgtcgtt ggcggcctgc agcacctcgt cctcgctgtc gaacatggtc agcggtgcga
    10381 cggggccgaa ggtttcctcg caggcgcagg ccatgcgggc gtcggcgccg ctgagcacgg
    10441 tgggggcgta gtaagtggcg ccgagctcgg gcaaacgctt gccgccggca agcaccttgg
    10501 cgcccttggc cacggcgtcc tgcacatggc gctctatctt ctcgaccgcg cggtcgttga
    10561 tcatggggcc gatctgcgag gccgggtcgc tggcggggcc gactttgagc gccgccacgc
    10621 gcgcggcaag tttctgcgcg aaggtatcga acacgccgcg ctgcacgaac acgcggttcg
    10681 ggcagacgca ggtctggccg ccgttcctga acttggcggc catcaggccg tccacggcgg
    10741 catcgacgtc ggcgtcgtca aagacgatga agggggcgtt gccgcccagc tccagcgaga
    10801 gttttttcag cgtatcggcg ctgcgccgcg ccaaatgctt gcccacgggg gtggagccgg
    10861 taaaggtgat cttgcgcacg cgcgcgtcgt cgagccacac atcgaccacc tcgggcgtct
    10921 tctcgcgcga ggcggtgacg atgttcagca cgccggcggg cacgcccgcc tcctgcgcca
    10981 gcagcaccag ggcgagggag gtgagcggcg tgtcctcggc tggcttgcag accacggtgc
    11041 agcccgcggc cagggccgga gcgatcttgc gcgcgatcat ggcggcggga aagttccagg
    11101 gcgtgatggc ggccacgacg cccaccggtt ccctgagcgc gaacatgcgc cggcccggca
    11161 ggggcgcggg gatgatctcg ccattcatgc gcgtggcctc ttcgccaaac cattcgatgt
    11221 agctggcggc gtaggccacc tcgcccttgc cctcggccag gggcttgcct tgctcacggc
    11281 tgatgagctt gcccaggtcg tcctggtgcg ccagcaccag gtcgttccag cgtttgatga
    11341 tggccgcgcg ctgtttggcc ggcaccttgc gccaggcggg gaaggcggcg tgggcggcgt
    11401 cgagcgcggc gcgggcctcc aaagcgcccg agtcgggcac ctcggtgatg gtggcgcccg
    11461 tggccgggtc ggtcacggcc aggcgggcgg cgggcgtggc gctccactgg ccggcgataa
    11521 agttggcgct gcggatgagg tcggggcggg tcagggtcag aggcatggtg ttatcagtca
    11581 aattggctgc taacgcttga tggataagcg cttgaagcta tggttttagc taacaatgcc
    11641 caggtgccag ggcacgaact cattgtctcc caatcccagt tgttcgctct tggtgggctc
    11701 gccgctggcg tggcgcagga tctgttcaaa gatgcgctgg cccatctcgg ccacgctgca
    11761 ttcaccgtcc acgatcaggc cgcagttgat gtccatgtcc tcggacaggc gctggtacat
    11821 gggcgtgttg ctggccagct tcatggtcgg cgcgggcttg ctgccgaaca tggagccgcg
    11881 cccggtggta aagcagatca ggttcgcgcc gctggcgatc tggccggtga cggccaccgg
    11941 gtcgtagccg ggcgagtcca tgaagacgaa gccatgctcg gtgatgggct cggcgtattc
    12001 atacaccgcg cgcagtggcg tggtgccgcc cttcatggca ctgcccagcg atttttcaaa
    12061 gatgttggcc aggccgccgg cctggttgcc gtggccgacc acgccgttga actgggcgtt
    12121 ctggccggcc gcgtagcgct cccaccaggc caggcggtcg agcagcttct gccccaccgc
    12181 cgggctgatg gcgcggcgcg tgagcatgaa ctccacgccg tggatctcgg gcgtctccga
    12241 caggatggcc gtgccgccgt ggcgcaccag gatgtccatg gccgcgccca gcgccgggtt
    12301 ggcggtgatg ccggaaaacc cgtccgagcc gccgcactcc aggccgatct tgatgtggct
    12361 ggcactgacc gtctggcgcc gcagctggtt ggcgatgggc agcatctctt cgatggcccg
    12421 gatgccggcc tctatggtga cgcgcgtgcc gcccacctcc tgcatcacca gggtgcgcag
    12481 cagctggccg ttggccgggt cgtccttgag gccctgcgat tcgaccagcg cgccgacctg
    12541 gttgcgctcg cagcccaggc cgacgatgag tgcgcccgcc aggttggcgt ggcgcgcgta
    12601 gccggccagc gtgcggcgca gcacgtcgaa atgctcgctg ggggaggaca tgccgcagcc
    12661 gctggtctgc gcaaaggcca ccacgccatc cacatggggg aaggccgcca gccgctcggg
    12721 cgtgaagtgc gcggcgatct gccggatcac cgtggccgag cagttcaccg acgacaggat
    12781 gccaatgaag ttgcgcgtgc ccacgcggcc gtcggcgcgc acatagccct ggaaggtggc
    12841 gcgctgctcc tcgggcaggt agtccacggg gcgcacgtcg gcaccgaagg cggggtcgcg
    12901 gtagtagtcg acgatcttca ggttgtggct gtgcacgtag tcgccgggcg ccaggtcgcg
    12961 cgtggccacg ccgatcacgg tgtcgaactt cttcacctgc tcgcccgcgg cgatgtggcg
    13021 cgcggcaatc ttgtggccgg ccggcacctg ggcgcgcgtg cgcacgccca gttcggagat
    13081 ggtctggccc agcgccaggg cggacttggc aacgaagacg ttgtcgtcgg ggtgcagatg
    13141 cagcaggggg ctgtcgatgg tggccatggc tctgtccttc agctgcgcat cagttcgcgc
    13201 agcttgagct tgtcgatgag cacggtttcc ttctggtaga ccgtattcac gtattgttca
    13261 tagtcggggc catctaggta gagcagcggg gcgtcaagct tgtccgtcac ggccttgaac
    13321 tcggcgctgg ccacggcctg tttgaaggcc tcgcgcagcc gggccaggat ggccggatcc
    13381 acgcccttgg gcgcgccgat gccgttgggg gcatcgacca ccacgttgta gcccagctcc
    13441 ttgagcgtgg gcgtgtccct gaagcgctgc gtgcgctgtt cgccccaggt ggccagcagg
    13501 cgcagcttgc cggcctcgac atgcggggcc caggagctgg agtcggccag cacatccaca
    13561 tggccgccga gcacgccctg cagcgcctcg gcgccgccct tgtaaggcac atggttcagc
    13621 tgaatgcccg cggccatggc gaactcttcc atgcccacat gggtggcgcc gcccacgccg
    13681 gcatgggcgt aggtgacctt gccagggttc gccttggcga aatcgacaaa atccttcagg
    13741 gtcttgaatt gcgagcccgg cagcacggcg atgccgaagg tctggcccga cgtgcgggcg
    13801 atatagctgt agtccttgcg cgggtcggcg gcgagcgtgc ctagctgcga aaaacgtgtc
    13861 accgagatcg ggatctggcc aatggtgtag ccatcgggcc gggccgatgc caaagccttg
    13921 gcaccgatca tgcccgaggc gcccacgcgg ttttccacgg cgatggcttg gcccagcacg
    13981 cgtgcggcca cggtgcacag ggcgcgcatc gactggtcgg ccgtaccgcc ggcgggccag
    14041 gggcagatga aggtgatgga gcgctcgggc caaccctggg cgagcaggcg ggcggggctg
    14101 ctggcgagcg ccgtggcgcc gagggcgccc aggatgagct gacgcctggt attgcgttct
    14161 atggtcatgc ttgtctccgt gtcgtgggta aagcgccatt gttggaactt ggtgcataac
    14221 aatccaatga aaatacgcga tgtattcatt gcaaaacagt tatgtacttg gtgaaccaga
    14281 aaggtggtga tcgatggcgt tgattgaccg tgtgctgcgc agcaacctga aactgcgcca
    14341 tttgcagatg ctggtggcgc tggatcagtt tcgccacctg ggacggacgg cggagttcct
    14401 gtcgctcacg cagccggctg tctccaagac gctggccgag atcgagtcca tgttcggcct
    14461 gccgctgttc gagcgatcca cgcgcggcac cgagcccacg gcggccggcg ccagcgtggt
    14521 gcgctttgca cgtttggtgc tggctggcta cgagcgcacg cgcgaggaaa ttgccgccga
    14581 ggccagtggc gccatgggcc gcaccagcgt aggcgccatg gtggcggcta cgcctgtgct
    14641 gctggcgcag gctgtgcagc ggctgaaaca gcattcaagc cgcacgacgg tactcatcga
    14701 cgagggcgac ctgacacggc tcctgcccaa gctgcgtctg ggggagctgg acttgttcgt
    14761 ggggcgcttg gagcctgcct atgcctcccc cgacctggag accgaggcgc tgtatgaaga
    14821 tccgatggcg gccgtggtgt gcacaggcca cccgctggca gatgtgcgcg ccgtgcagtg
    14881 ggccgacatc gccgcccagc cctgcgtgct gccgccgccc tgggcctcgc tgcgcgtgaa
    14941 gctggagcag cagttctttg cccacgagct gcatccgccg gcggacatca tcgagtcggc
    15001 gtccttcctg gtgcagctca ccttcatgca gcagcgcggc gccgtggcgt tcatggcgcg
    15061 cagcgtggcg cgccaccacg cgcagctggg catggtggcg ctgctggacc tgcccgtgcg
    15121 catagcgctg ccgcccgtgg gcctgatcac gctgcgcgga cagcgcatga ccccggtgac
    15181 ggcggcgctg gtggaatgcc tgcgggacga ggccaggtta ttggcgcagt agttcagcgc
    15241 ggtgcgcagg agtcgcccag gccggccacg ctggccagtt tgtccaggcg cgagcgctgg
    15301 tccaggcagg cctggcgcgc ggcctcggag gccttttgct gcgccaggcg cgtggcctgt
    15361 gcgcgctcgg cctgcagctg cgcaatgcgt tcgcggatct gcgccatggc ggccagggcg
    15421 gccttctcgc cttcggcgat ggcctgggtg cgctggttga agtccaccgg gcccatgtcc
    15481 agcacctgcg ggcgtatcac cacgtcggcg cgtgccagct cggcctggcc gagcttctgg
    15541 cccatgatgg cgatcgactg gttcagcgcg cccagcatgt gctcgggcgt cttgccgcgc
    15601 gccttgttgg agatgtccac ggcgatcacc atgtccgcgc ccagctggcg cgccgcatcc
    15661 accggcacgg ggctgaccac gccgccatcg acgaagtggt atttgccaat gctgaccggc
    15721 tggaaaacgc ccggcacgct gctggaggcg cgcacggcct gaccggtgtt gccgcgcgca
    15781 aacagggtgc gctcgccgtc ctccagccgc gtggccacgg ccacgaaggg cttggcgagc
    15841 tgctccaggc tcttgcggcg cacctgttcg ttcacgtagt cctccagctt ctggccctgc
    15901 accagcccgc ccgaggacag ctgcaggtcg cgcaccttgg actcgtccag cgccacggcc
    15961 ttgtcctgca gctcaaacgc gttcatgccg ctggcgtaca gcgcgcccac cacgctgccg
    16021 gcgctggtgc cggccaccac cgccggcgcc aggccgttgg cctccagcat cttgatcacg
    16081 ccgacatggg caaagccctt ggccgcgccg ccgcccagcg cgatgccgat cttgatgggg
    16141 gcgggcgggg cggtggccgt ggcctgcggc gtgatcgtgg gctggctcgg cgtggtgttg
    16201 ctgcatgcgg ccagtccggc caggccgatg gcggtcagtg ggcgccagac agcatgccgg
    16261 cgcatgccag aaaatcctgc attcataggg gcggggagta tagggcgggt gattctcggc
    16321 cttgtcggca atgctgttct ggtgcgaaga gatcagggca aaaatggccg ttttcgctta
    16381 tctggcaagc gctgatagct attaaattga aagtggcttg atggtgcttg ccatgccggg
    16441 caccgcgcgc tgcgccgctc agcgcatggt cacgaattcc tcggccgccg tcgggtggat
    16501 ggctacggtg tcgtccaggt cgcgcttggt tgcgcccatc ttgagtgcca ccgcaaagcc
    16561 ctgcaggatc tcgtccatgc catggcctat gccgtggatg ccgacgatgc gctcctcggg
    16621 gccggcgcag accagcttca tgcgcgcgct ctggcggtgc cgggtcacgg ccgtgtacat
    16681 ggccgtgaac gccgcctggt agaccttgac ctgggcgtcg ccaaagcgct cgcgggcctg
    16741 gggctcggtc agtcccaccg tgcctatggg tggatggctg aagaccacgc tggggatgtt
    16801 ctcgtagtcc agatgctcgt ccggccggtt gttgaacagg cgctcggaca ggcgccggcc
    16861 cgcggccacg gccacgggcg tgagctcgat gcggccggtg atgtcgccca cggcgtagat
    16921 gccgccgaca ttggtgttct ggaacttgtc cacctgtatg tacccgcctt cgtccagcgc
    16981 cacgcccgcg gcctccagcc ccaggcccga ggtgtcgggc ctgcggccta tggcccagac
    17041 gatgcagtcc acgttgtggc tgcggccatc ctccagccgc aggcacaggc tgccgtcggc
    17101 attgcgcgtg acggactggg gcgtggcatg gcggtgcagc gtcgggccct cggcctccat
    17161 gacctgcatc agcgtctcgc ccagcatggg atcgaaatgg cgcagcgccg atgcgcggcg
    17221 cagaaacaaa tgggtctgcg agcccaacgc gtggagcacg cccgccagtt ccacggcgat
    17281 gtagccggcg cccacgacgg ccgtgcgcgc gggcagggcg ggcagggcga agaagccgtc
    17341 ggagtccagg cccagctcgg cgcccggtat cgccggccat gtgggacggg tgccggtggc
    17401 gatcaggata tggtcggcgc tcaggcgccg gccctcgacc tcgatggtgc gcgcatccac
    17461 gaagcggccg aagccacgga tcacatccac cttgttgcgt tgcaggccac tgtcgtagga
    17521 ctcgtggatg cgcgcgatgt aggcgctgcg gctctcgatc aggcgctgcc agtcgaagcg
    17581 gttcacgcgg gtgtcatagc catagtcggg gccgtagcgg tgtatggctt cggcgatctg
    17641 cgccgcatgc cacatgacct tcttgggcac gcagccgaca ttgacgcagg tgccgccgat
    17701 ctcatgggcc tcgatcagcg cgcatcggcg cccgtacatg gcggcgcggt tggccgatgc
    17761 gatgccgccg ctgccgccgc cgatgacgat gtagtcgtag tgcttcatcc cggactcctg
    17821 ctgtttggct gtcttgcaac ggggccatct tgccctgtcc tgcaagcagg gggccgagca
    17881 ggggcaatgc cggcgccggc gccggctggc ggcggtgggc gtcagccctt ggtgatcgtc
    17941 gccgcgatgg ccttgcccac ctgctgggtg ctggcctggc cgcccaggtc gggcgtgcgc
    18001 ggcccgctct tgatgacctg ctcgatcgcg gccacgatgg catcgtgcgc ggcgcggccc
    18061 gcgccctggc cttgcgtcaa aaagtccagc atcagcgcgc ccgaccagat catggcgatg
    18121 gggttggcga tgttcttgcc gtagatgtcg ggcgccgagc cgtgcacggg ctcgaacagg
    18181 ctggggaagc tgcgctcggg gttcaggttg gcggacggcg ccaggccaat ggtgccggtg
    18241 gtcgccggcc ccaggtcgga gaggatgtcg ccaaacaggt tggtggccgc caccacgtca
    18301 aagcgccccg gctgcagcac aaagcgcgcc gtgaggatgt cgatgtgctg tttgtccagc
    18361 gccacctcgg ggtagtgctt ggccatgtcc tcggcgcgct ggtcccacca gggcatgctg
    18421 atggcgatgc cgttgctttt ggtggccagc gtcacatgct tcttcgcccg gctttgcgcc
    18481 agctcaaagg cgaacttgag caggcggttc gcgccgtggc gcgaatagac cgattcctgg
    18541 atgacgatct cgcgctcggt gccctcgtac atgatgccgc ccagcgaggt gtactcgccc
    18601 tcggtgttct cgcgcaccac gtaatagtcg atgtcgccgg gtttgcggcc ggccagcggg
    18661 cagggcacgc cttcgaacag gcgcaccgga cgcaggttga tgtactggtc gaactcgcgg
    18721 cggaacttca gcagcgaacc ccacagcgac acatggtccg gcacggtggc cggccagccc
    18781 acggcgccgt agtagatggc gtccatgcct tgtagctgcg ccttccagtc gtcgggcatc
    18841 atctttccgt gggccgcgta gtagtcgcaa tgcgcccagt cgaagtggtg gaattcgagc
    18901 gacaggccaa agcgttgggc ggcggcctcg acggcgcgca ggccttcggg catgacttct
    18961 ttgccaatgc cgtcgccggg caggacggca atcttgtagg tggtttgcat ggggtgctcc
    19021 aaggggtgat gggcgccatt gttcactgat cacgaggttc atacaatgag ccatttcgtg
    19081 gtttcattgt tcacacatcg tgcagaaaat accacccacc gacgatttgc gcgtgttctg
    19141 caccgtggtg cgcaaggccg gctttgccgc cgccgcgcag gagctgggag cctcgcccgc
    19201 ctatgtcagc aagcgcatcc gcctgctgga gcaggaactg ggcgtaccgc tcttgcaccg
    19261 caccacgcgc cgcgtagtgg tcaccgacca gggcgagcgc gtgttccact gggcgcagcg
    19321 catcctggac gacatggatc agctgctgca ggaggtagcg gtcacgcgcc gcgagccgcg
    19381 tggcctgctg cgcgtctcca gcagcttcgg ctttggccgc aacgtggtgg cgtcggcgct
    19441 gtcgcgcctg gtgcgccagc acccgggtct gcaggtgcgg ctggaggtgt tcgaccggct
    19501 ggtggacgtg gcggccgagg gctttgacct ggacgtgcgc gtgggcgacg agatcgcgcc
    19561 gcacttgatc gcgcggcgcc tggccgacaa ccaccgcgtg ctctgcgccg cgcccagcta
    19621 cctggcgcgc cgcggcacgc cgcgcacccc ggccgagctg cccgcgcacg actgcctggt
    19681 gatcaaggag cgcgaccacc cgttcggcgt ctggcgcctg cgcgcgggtg cgcaggagca
    19741 gtcgctgaag gtaacgggcc cgctgtcggc caacaacggc gagatggtgg tgcagtgggc
    19801 cgtcgatggc tgcggcatcg cgctgcgttc gcgctgggat gtgcagcagc acctgcatgc
    19861 cgggcgcctg gtgcagctgc tgcccgagtg gcgtcaggag gccaacgtgt gggccgtcta
    19921 ccccacgcgg ctgcagctgt cggccaaggt gcgcgtgtgc gtggagtttc tgcaggcgta
    19981 cttccggcag gaatgggggt gattgcgggg tgcccgctgt gcactacagg tcgaaatcgc
    20041 ccgcggcttc tggctggtaa agcactttct caatcgaaat gcggcgcgtg cggttcggaa
    20101 tccgccagtc gaaggcgtca ccctccttgt agcccaggat ggcggtgccg atgtcggagt
    20161 aaaccgacag ctttccggca ctgtcgtcgg cgtcctgtgg gtagaccagg gccacctcca
    20221 ctttctcgtc gtccagatgc agcaaggccc tggagttcat cgtgaccaca ttgcgcgcca
    20281 cttgccgggc atcgacgacg atggcgcgtt cgatctcgtg ttccagatcg accacatgcg
    20341 cgtccagctc cagcgccacc aggtcccgca gccgcgcctg gtcgacttcg gtgatgtgga
    20401 tctggcagga gtcgcccatg gtgcctgcgc gcaagtgctg gagatagtgc gccgaggtca
    20461 tggcatagga cttcagtgtg ggcgttgcgc cgtcaggcag aaatccgcag cgctgaaagg
    20521 ccttcagcga gcgcgtgttg tcggcgtgga tcttggcaat cagccgctcg gcgcgcatgt
    20581 cgaagaaggc cagcttcatg ccctcgcgca gggcgctggc gccaagcttg cggccccagt
    20641 tgtcgcggct gccgatgagc aggacgatct cgcagtcagg cccgttcttg accaggcgga
    20701 caaagcccac cggctggtca tgccggtcgt aggccatgaa gaagcggccg ccctggttga
    20761 acagatgggt caggatcggc agctgaaccc ggccgatgac ctgttcgatg aaccgcgaga
    20821 catggcgcga atcgctcagg tagcgggtga cgctctcgtc ctccagccag tccatcaaat
    20881 gcagcgcgtg tgcccgcgtg atctccgggc acagagaaat gaaaggcttg ctcatcttca
    20941 ccacggttgg ttgcaagaat gaaagccttt catggccgtg gccatgacgg ttctttcgac
    21001 agggcggcca tcttagtgca gtgcgcgcga gcggggcgtg atgccctgca gtagccgcat
    21061 ggggcgtgcc gttgttccaa ataaaaatag cttcaaacgc ttgtttatca agcgctcgaa
    21121 gctattaaaa taatagtaac tgggggctaa gaccgaatcg atgctcaacc cgccagcgcg
    21181 gccttcacgg cgctggatac ctggcccatc tcggccttgc cggccaggcg ggtcttgacc
    21241 acgcccatga ccttgcccat gtcgcctggg cctgcctggc gtccgagttc cgcgcccagt
    21301 tcggcgacga tggccttgac cgcgtcgagg atctcggcct cggacatgcg cgccggcagg
    21361 taggcctgca gcacggccat ttcggccttt tccttgtcgg ccagatcctg gcggcccgcg
    21421 ccctcaaacg ccgtgatgct gtccttgcgc tgcttgatga gcttgtccac gatggcgacg
    21481 atggcggcat cgtccagagt gatgcgctcg tccacctcct tttgcttcat ggccgcctgc
    21541 agcaggcgga tggtgcccag gcgctcgctg tccttggcgc gcatggcggt tttcatgtct
    21601 tcggtgatct ggtctttgag gctcatgggg tttctccaag tcaaaaaagc aaaaacccgc
    21661 gcttggcgtc cctagcgcgg gttttgtggg cgtcagtaaa aacgccgggc tgtgcttagt
    21721 acagcttctt gggcagctgc atgctgcgca cgcgcttgta gtggcgcttg acggcggcgg
    21781 ccttcttgcg cttgcgctcg gcggtgggct tttcgtagaa ctcgcgggca cgcaggtcgg
    21841 tcagcaggcc cagcttctcg atggtgcgct tgaagcggcg cagggcaacg tcaaaaggct
    21901 cgttttcttt aacgcgaatg gtggtcatta gctaaggtgt ttccaaaata tgcccacaga
    21961 gggtagccag gaagatcggg ccgggctgcc atgtgcggcg ggttccccgt cttgtaacta
    22021 gtatccggcc gacggggttt gccagcaaag ccaagcatta tagcgataag ccgcatgcga
    22081 gcaaggctat gcgcgccgca agccggcggg tgggggttga aaggcgacaa tagccctggc
    22141 gcgcatacag ggacagcctg gcgctttgct gaatctgcaa aagattcata ccatatgaat
    22201 gtttaaacgc tcatcgaatg gccatgtcta caccgtcaga gaatttggat acgcccgtag
    22261 agcaggcgcc ggcgcttgag caggcgcttg cgcgccccta ccagcatggt tttgtcactg
    22321 acatcgagtc cgtcagcctg ccgccgggcc tggatgagga caccatccgc tccatttcgc
    22381 gcatcaagcg cgagccgcaa ttcctgctcg actggcgact ggcggcgttc aagcgctggc
    22441 aggcgatgga gctgcccgac tgggcgcggc tgaacatcgc gcccgtcgac ttccaggcgt
    22501 tgtcgtatta ctcggcgccc aagtccatga aggatcgccc caagagcctg gacgaggtcg
    22561 atcccaagct gcttgagacc tacgaaaagc tcggcgtgcc gctgcacgag cgcgcggcgc
    22621 tggccggcgt ggcggtggat gcggtgttcg actcggtctc ggtgggcacg acgtttcgca
    22681 agcagctggc ggacgtgggc gtgatcttct gcagcttctc gcacgcggtg cagaaccacc
    22741 ccgagctggt cgagaagtac ctgggcacgg tggtgccgca gggcgacaac ttctacgccg
    22801 cgctcaactc ggcggtgttt tccgacggct ccttcgtcta catccccaag ggcgtgcgct
    22861 gcccgatgga gctgtcgtcc tacttccgca tcaatgccca gaacaccggg cagttcgagc
    22921 gcaccttgat cattgccgag gagggcagcc aggtgagcta cctggagggc tgcaccgccc
    22981 cgcagcgcga cgagaaccag ctgcacgccg ccgtggtgga gctcgtcgcg catgacgacg
    23041 ccttcatcaa atattcgacg gtgcagaact ggtacccggg tgatgagaac ggcaagggtg
    23101 gcatctacaa cttcgtcacc aagcgcggcc tggcggcggg caagcgctcg cgcatcgcct
    23161 ggacgcagat cgagaccggc tcggccatca cctggaagta ccccagcgtg gtgctgcgcg
    23221 gcgatgagtc gaatggcgag ttccactcca tcgccgtgtc caaccactgg cagcaggcgg
    23281 acaccggcac caagatgatc cacctgggca agaacaccac cagccgcatc gtctccaagg
    23341 gcatcagcgc cggcaacgcg caaaacagct accgcggcct ggtgcgcatc aaccccggcg
    23401 ccgccggcgc gcgcaaccat acgcagtgcg actcgctctt gattggcccg cactgcggcg
    23461 cccacaccta tccctatgtg gacacggcgc gcagcgacgc cgtggtcgag cacgaggcca
    23521 cgaccacgcg catcagcgag gagcgcctgt tttattgcga gcagcgcggc atagacgcgc
    23581 aggacgccac ggcgctgatc gtgggcggct tctgccaggc cgtgctggag gagctgccga
    23641 tggaatttgc cctggaggcc aaggccctgc tggccgtgtc gctggaaggc gcggtaggct
    23701 gatattcaga taaaaacggg ctgtagcgct tatgtataaa gcgcgagaag ctatcaaaat
    23761 aataggaacc cacatgacac acaccccacc cctgctgcaa gtgcgcgacc tgcgcgtgaa
    23821 cattggcgag cgcaccgtgc tgcattccgt atcgctggac gtgcagcccg gccagctcgt
    23881 cgtgctgatg ggcgccaacg gcagcggcaa gagcacgctg ggcatggccc tggccggcca
    23941 tccgcactac caggtgcagg gcggtcaggc gctgctggcg gggcaggatc tgctggcgct
    24001 gccggcgcat gagcgcgcgc gcgccgggct gtttttgtcc ttccagtcgc cgcccgatgt
    24061 gccgggcgtg aagaacaacc tgttcatccg caccgcgctg aatgcccagc acgaggcgcg
    24121 tggccaggag ccgctggatg ccttcgactt cctctccagc gccaagaagg ccgccaagga
    24181 tctgggcctg cccgaagcca tgctgggccg cccggtcaac gagggcttct ccggcggtga
    24241 gcgtaagcgc aacgagctat tgcagctgtc gctcttgaag ccgcgcctgg cgctgctcga
    24301 cgagatcgac tcgggcatgg acgtggacgg cgtgcgcgcc gtcgtcgccc tggtgcaaaa
    24361 gctgcgcgcc gagggcacgg ccttcatcgt catctcgcac tacctgcaga tgatcgaatc
    24421 cctggagccc gatgccgtgc tgcgcctgca ccagggctgc attgccgaga ccggcgacct
    24481 ggagctggcg cgcgagatcg cgcgtaccgg ctttgcgcgc gcgccgcagg ccgtggaggc
    24541 atgatggaca gcgcaagagc cgacatcctg gccgcgcgcc agcacctgca gcagcagggc
    24601 tggatcgcgc gcaagagcga agcctttcac cacctgccgc cgccggcgct ggaccaatgg
    24661 ctgggcgcgc aaggcgagag cggttgcgag atcccggccc tgagcggcgc gggctggacg
    24721 ctgcaccccc tgcatcgccc ggacggcaac cacggcgcca gcggcatcca tgcgcgctgg
    24781 ctcgatgccc acgaccccgc cgagcgcagc gagctgtttg ccggcctgcc acggccgggc
    24841 aacacaggcg ttgacggcga ggccgcgccc ttcgcctggg cgcaccgcgc gctgtgccgc
    24901 ctgggcctgc gcctgcgcat cagcgccacg ccgggcaagg agcgcgaacc cgtctggctg
    24961 gagctgcgcc accagccgcg cgcccgggcc gaggcgccgc tgctggtgct ggaggtggac
    25021 cctggcgtgc aatgcgtgct catcgaaagc catgagcgcg aggtcggcag ctgccagcac
    25081 gcggtggcgc aaaacctgca cgcccatatc cacctgggcg ccggcgcgca actgcagcac
    25141 ctgcgcatcg ccgcgcccgt ggccgatgac agcgtggcgc accacctgca catcaacgtt
    25201 gccgccggcg cgcagtacgc acaggcgctg gtcgccacat cggccggcta ccacctgcag
    25261 cgcagcctgg tgcagctgca gggcaagggc gcgcaggcgc acagcgcggc gctgctgctg
    25321 gccggcgcgc acaagataga ccaccagagc tacacccgca tgcaggccgc gcacagcgcc
    25381 agcacggtgg agacgctgac gctggcccag ggcaaggccc atgcggtggc caatgcccac
    25441 accaccatcg cccccggcgc cgacgaggcc aacgtgcgcc agcgcctggc gggcgtgccg
    25501 ctgtcgggca acccgcgcct gatcctgcgc ccgcacctgg agatattgca cgacaacgtg
    25561 caggccgcgc atggcgccac ctggggcgcg ctgcccgagg acgcgctgtt ctacgcccgc
    25621 cagcgcggcc tggacgaggc cagcgcgcgc agcctgatca tcgaaggcat ggcgcgcgcg
    25681 ctgctgcagc gctgcctggg ccaggacgac gacggcttgc tggcgcagtg gctgggcggc
    25741 ggctggctgg cgcgcgccat agcggtgcag ctgcaggcgg atatggagga gcggcatggc
    25801 taacacattg gctgagcatg tgggcgtcgc cggccctcac ccccaccctc tcccaggggg
    25861 agagggggtt gataccggcg cggcctcgct ccctcgcccc gctggggaga gggtaggggt
    25921 gaggggccag ccaggcgagc aagagcagca agacctgagc cacctgcgcg cccaattccc
    25981 catcctggcc caatcgatcc acggccaccc cctggcctat ctggacaacg ccgccaccac
    26041 gcaaaccccg ctggccgtgc aacaggccat gcagcagttt gaacagcgcg accgcgccaa
    26101 catccatcgc ggcgtgcaca ccttgagcca gcgcgccacc gacgctttcg aggccgcgcg
    26161 cgccacgctg aaggccttcg tcggcgccgg cccggcgcat gagctggtct tcaccagcgg
    26221 caccaccgag gcgctgaacc tggtcgccca gggcctgtgg gccgcggggc cgggcaaggc
    26281 ctggctgaaa cccggcgacg agatcatcgt cagcggcctg gagcaccacg ccaacatcgt
    26341 gccctggcag caggccgcgc gcatgagcgg ggccaccctg tccgtgctgc gccccgacgc
    26401 cgagggccgc atccatacgc aggatctgca gcgcctgctg ggcgcgcgca ccagggtgtt
    26461 ggccatgacg gccggcgcca atgccaccgg cgagcgcccg ccctacgaag aaatgctggc
    26521 cctggcgcgc caggctggcg ccctgacggt gctggacgcc gcccaggccg tgggccacgc
    26581 catgcccgct ctgtcggccc tggactgcga cttcatggcc ttctctggcc acaagatgta
    26641 tggccccatg ggcacgggcg ccttggtagg caagcgcgag gtgctacaac ttttataccc
    26701 attgcgcctg ggcggcgaca tggtggactg ggtcagctac gaggcagccg gctacgccca
    26761 gctgcccgca cgcctggagg gcggcacgcc caacgtggcc ggggccgtgg gtctggcggc
    26821 cgccgcgcgc tttatcgacg aggtggggcg caccgccatc gaccagcacc tgcatgccct
    26881 gcgcgcgcag gcgctggccg gcttgcaggc aatagatggc atcagcgtgc tggcgccgca
    26941 tgccacgcag gcctcgctgg tgtcctttgt ctcgcacgac gtgcacccgc acgacatcgg
    27001 cacgctgctg gacgagcgcg gcatcgccgt acgcaccggc caccactgcg cccagccgct
    27061 gctggatcac ctgggcctgg ggccgaccac gcgcgcctcg ttcgcgctct acaacaccag
    27121 cgacgaggtt gatcgcttcg tcgccgccgt ggcccatgcc
//
LOCUS       DAOMTQ010000021        27115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_168_length_27115_cov_22.944715, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000021 DAOMTQ010000000
VERSION     DAOMTQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 27115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27115
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_27115_cov_22.944715"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PK518_03720"
     CDS             complement(<1..1000)
                     /locus_tag="PK518_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM48049.1"
                     /translation="MNFSLPPELRALRDKTRQFVAEQVIPLENDERQSHHGPSEALRD
                     ELVARARAAGLLTPHASPEMGGLGLSHVEKAVVFEEAGYSWLGPTALNIHAPDEGNIH
                     LMEAVATPVQKERWLRPQVAGATRSCFVMTEPAPGAGADPSMLATTARRDGDDYVING
                     RKWFITGAEGAHYAIIMARMEDGSATMFLSDMDRPGITLERNMDAMDSCFAGGHGVLR
                     FDDLRVPATDVLGEVGQGFRYAQVRLAPARLTHCMRWLGQARRAHDQALAYARRRQAF
                     GRPLAEHEGVGFMLADNDMDLHTARLHIWHKAWLLDQGEKCNFESSRAKVVCSEAEW"
     gene            complement(1076..1999)
                     /locus_tag="PK518_03725"
     CDS             complement(1076..1999)
                     /locus_tag="PK518_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRM48050.1"
                     /translation="MFLLQSPQVCELEPFTSMPEPLRRRERSDWADANRGGVVTDSFL
                     EGPVWGDDGNLYVTDIPWGRVFRIDPQGAWTLAAEYDGEPNGMKFLDAGTLLITDYKN
                     GLMRLDLASGQVSPFLERRNSERFKGVNDLVFDSQGNLYFTDQGQTGLHDPSGRLYRL
                     RTNGQLDLLLTNVPSPNGVALSPDERVLYLAVTRGNCVWRVPLLPDGSVAKVGQFFTS
                     YGPSGPDGLAVDAEGRLLVANPGLGYVWVLNDRAEPVQVMRGLPGSSITNLAFGGADR
                     GQIYVTDSTHGRILRATLGVPGLPLHRAARH"
     gene            complement(2012..2875)
                     /locus_tag="PK518_03730"
     CDS             complement(2012..2875)
                     /locus_tag="PK518_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM48051.1"
                     /translation="MTAHTVPHSVGLNRPARRLPPLACDSHMHIFDPRFAPSPHWKRT
                     PPDAPVAAYRRLQQRLGTQRTVVVTPSTYGTDNACTLDALDQLGDGARGVAVVAQDVG
                     DAELDRLHARRVRGLRVNFVTPQSWGETTPQMLATLARKAARLPLWHIQVFMHPEQIV
                     ALESVLAALPVPLVIDHLGRIDPDEGPSADGYGALRRLLDGGNTWVKLSGAYMRSTAP
                     AYADTLVLAQALVRAAPERLVWGSDWPHTTAAPGTVNDVDLVDLLRAWAGSDAAMDRI
                     LVDNPARLYGF"
     gene            complement(2872..3843)
                     /locus_tag="PK518_03735"
     CDS             complement(2872..3843)
                     /locus_tag="PK518_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48052.1"
                     /translation="MNRMNFPLCAALALACAAAGVQAQTAYPDKPVKIVVPYPPGGAV
                     DQVTRRIAQKLTEQTGQSFFVENKAGATGTIGAAQVARSPGDGYTLTANDTTYSILPQ
                     VFKKLPWDYAHDLVPVAGFNFAPTTVVVAANSPFKTLGDMVAYSQAHPGKLNYGTGGA
                     GTMPHFATVALEGVSGLRATHVPFKGAGEATLALLSGTIDFQIASTPGVMGQVRGGKV
                     RLLAVSGEQRLKALPDVPTFAQAGAPDYKVVNFTGLWAPKGTPEAVLETLRKNIAKAM
                     ASADVQSFANDLGAVPGVVSGQAFADKLKSDAQLWEAVAAKVGIDKQ"
     gene            complement(3866..4855)
                     /locus_tag="PK518_03740"
     CDS             complement(3866..4855)
                     /locus_tag="PK518_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48053.1"
                     /translation="MLKQNRLLALAEKALTAILLVASGACFAQAGYPSKPVKLVVGFA
                     AGGPTDVVARAFADHAARALGQPFVVENKPGANTILAAQAVASAPADGYTLLFGATNH
                     TMIPALYGTRVKFDAVKSFQPLCTVATSPTVLVVGPGMPVKSLADYMARARKEPRRTT
                     AGTAGVGSSGHFATEMFARANGLQLNHVPYKGAAPVVTDLMGAQLDSSFATLGSVLPQ
                     LREGKLAALAVASPKRSPLLPGVPTFAEAGGGNYSADAWYGVLAPAGLPQPIAQQLEK
                     AARDFAASTAAAEKLRGLGLDADSTCGLAFAQQVEREVATYMQIARDLNLKAE"
     gene            complement(4917..5885)
                     /locus_tag="PK518_03745"
     CDS             complement(4917..5885)
                     /locus_tag="PK518_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48054.1"
                     /translation="MNRRNFNVLAAALAAFTAIPATAQSAWPNKPITLVVPYAPGGTA
                     DALARVLAQHLGTRLKVSVVVLNKAGASGVIGAASVAQAPADGYTVLYDATPLSINPH
                     LQKLPFDAAKDLQPVALVGVTPMLVVVPKNSPYNTLQELIAAAAKASGKLTFCSGGQG
                     TVQYMGAELFNQGVGIKMLHVPYRAGGLALQAILAGEVDMGFFNLPALSSHIKGGALK
                     PLAITSAKRNPLFPNVPTISEAGVKNYEVYEWNGMFVPSTTPAEIVTRLNTAVREVLA
                     MPEVKARFDALGSEIVGSQPDEFRKRLAAESARWAETIKAAGIKKE"
     gene            complement(5923..6852)
                     /locus_tag="PK518_03750"
     CDS             complement(5923..6852)
                     /locus_tag="PK518_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM48055.1"
                     /translation="MSRAAILVTAADLAPQALALLADYDIVYAGKTPTEDEVVALCRR
                     HNPVAIIVRYSKVGAAAMDAAPSLKVISKHGSGTDTIDKAAAQARGIEVVAAVGANAA
                     AVAEQALALLLACAKSVPQLNERMHAGHWDKATHKSVELGGRTIGLVGLGAIGLRFAK
                     MADALGMRVLGFDPFAKNLPDFVQAADLETIWRESDAISLHCPLTDDNRDLLDADTLA
                     RCKKGVIVVNTARGGLIDEAALLAAVHSGQVRMAGLDSFAVEPMAAGHPFQHQSGFIL
                     SPHIGGVTSDAYVNMGLGAARNALAVLNRTATA"
     gene            complement(6849..7535)
                     /locus_tag="PK518_03755"
     CDS             complement(6849..7535)
                     /locus_tag="PK518_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRM48056.1"
                     /translation="MNQLPEVIRTIERVSPDVVQQAATFQAAILADVAGRRGTMHARV
                     APVHERMRLAGPAFTVEVRPGDNLMIHAAIALAQPGDILVIDGKGDQTAALMGTLMLS
                     ACKKRGLGGVIVDGCIRDKLELLELDFPVFSAGFNPAGPTKFVPGRVNHPIACGGAMV
                     HPGDLVVGDADGVVVIERAKAPAMLALAVKKVADEAARIESIARGDTAAKWLPAALRA
                     AGVLKEGETL"
     gene            complement(7634..8635)
                     /locus_tag="PK518_03760"
     CDS             complement(7634..8635)
                     /locus_tag="PK518_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48057.1"
                     /translation="MAFSRRFSLTASLGATLLGLLAAAVPATGAMAQTAYPAKPIRLI
                     VPFPPGGGTDMIARTVAQKLTDQNRWTIVVDNRPGAGGNLGVDAAAKAAPDGYTLVVG
                     QTSNLAINPSLYPKLPYNPLKDLVPVALLSSSPIVMAAPAGSPYKSFADVVAAAKANP
                     DGITLGYSGNGTVAHLSGELVENAAGIQLRHVPYKGAAQAMTDLVGGQIDLYMSSVPT
                     LLGQVRNGKLRAIVLTSAKRSDQLPGTPTLEESGYKGFESVTWFGLLAPAGTPAAIVQ
                     QLNKAINLALQQPDVAEKLRSEGGEVLGGSTEKFDQLLRAELPRWAKTVKASGVSLD"
     gene            8767..9546
                     /locus_tag="PK518_03765"
     CDS             8767..9546
                     /locus_tag="PK518_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM48058.1"
                     /translation="MSRPANPPLSADESTPAGARDGGVIAVTRALRLLESFAVGESQL
                     SLAEISRRCQLHKTTVLRLARTLAQSGYLVQREDGDWRLGPAAGWLGARYQAGFDVQN
                     VLEPVLRELTLATGESAAFYVREGDMRTCLVRVEGPQALRHHARMGEGLPLDKGSPGR
                     VILAFSGEPGEVYEEIRRRGYHWSIGEREQGVATVSAPVFGRHWRLLGCVCISGPQSR
                     LPQEKLEALAQTIIRTANQLSYALAGKAASTAAQAPGVWHP"
     gene            9604..10242
                     /locus_tag="PK518_03770"
     CDS             9604..10242
                     /locus_tag="PK518_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM48059.1"
                     /translation="MLHYLTIPVTAFQQNSSLVWCDQTMEAAVIDPGGDLDLLLAEAQ
                     RLGLHLKAIWLTHAHIDHAGGTGELAQKLGLPIIGPHPGDQFWIDGLPQQSQMFGFPP
                     ALHFTPTRWLHDGDTVTIGHETLNVRHCPGHTPGHVVFHAPQIDRCFVGDVLFAGSIG
                     RTDFPQGNHQQLIDSITGRLWPMGDQTVFIPGHGPESTFGRERRSNPYVGDT"
     gene            complement(10318..10509)
                     /locus_tag="PK518_03775"
     CDS             complement(10318..10509)
                     /locus_tag="PK518_03775"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HRM48060.1"
                     /translation="MPFAQIYMLEGRTEEQKKAVIEKVTQALVDAVGAPVANVRVWIH
                     DVPKENWGIAGVSAKELGR"
     gene            complement(10537..11520)
                     /locus_tag="PK518_03780"
     CDS             complement(10537..11520)
                     /locus_tag="PK518_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48061.1"
                     /translation="MQRRDFITAGALAACGASLAGGAWAQSGYPARPVRMVVPFPPGG
                     PTDVMGRTAAKAMGERLGQQFVVENKAGAGGNIGTDFVAKAAPDGYTIGLAAISSLAI
                     APHLYASLPFNVEKDLLPISLVGTTPCALVIHPGAPFSDLAGMVAHAKANPGRLSYAT
                     SGIGTSNHLAAELLQSVAGIALTNVPYKGSSQIVPDLLAGTVPMSMESSLATTLQHVR
                     AGKLKAIAVTSPQRAKALPDVPTVAESGYPGFEVETWFGLVAPAGTPPAVVAQLHDAW
                     EAGARTPQAQAAFDNISGNLRVNTPQQFADFIRAENRRWGGLIRQLGLKAD"
     gene            complement(11553..12341)
                     /gene="dmpH"
                     /locus_tag="PK518_03785"
     CDS             complement(11553..12341)
                     /gene="dmpH"
                     /locus_tag="PK518_03785"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HRM48062.1"
                     /translation="MALSKDTIERLAEHLEACQLQVRDTPKITDEHPDMDWDDAYAIQ
                     DAILARKLARGARVVGLKAGLTSHAKMKQMGVTDPVFGFLVDDYSVPEGGTVQTRELI
                     HPKVEPEIVFVLKHALKGPGCHIGAVLAATDFVLPGIEVIDSRYRDFKFDLKSVVADN
                     TSAARFVIGGQAQRPESVDLRTCGIVLEKNGEPVALGAGAAVLGHPAAAIAMLANHLG
                     RRGRELPAGSMILSGGVTEAVAVQAGDNVSLRVQGMGSVSLRFA"
     gene            complement(12357..13385)
                     /gene="dmpG"
                     /locus_tag="PK518_03790"
     CDS             complement(12357..13385)
                     /gene="dmpG"
                     /locus_tag="PK518_03790"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HRM48063.1"
                     /translation="MTQKITLHDMTLRDGMHPKRHLMTLEQMKSVAQGLDAAGMPLIE
                     VTHGDGLGGASVNYGFPAHSDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAHELG
                     VHTIRVATHCTEGDVSEQHITQARKLGMDTVGFLMMAHMNDAAGLVKQAKLMEGYGAN
                     CIYITDSAGYMLPGHVTERISAVRQALKPETELGFHGHHNLAMGVANSIAAIEAGANR
                     IDAAAAGLGAGAGNTPMEVLVAVLDRMGIPTGVHVWKIQDVAEDLVVPLMDFPIRIDR
                     DALTLGYAGVYGSFLLFAKRAEQKYGVPAREILLELGRRGMVGGQEDMIEDTAITMAR
                     ARGLAVAA"
     gene            complement(13541..14449)
                     /locus_tag="PK518_03795"
     CDS             complement(13541..14449)
                     /locus_tag="PK518_03795"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HRM48064.1"
                     /translation="MSDKIKCALIGPGNIGTDLLAKLQRSPVLEPVWMVGIDPESDGL
                     KRASEMGIKTTDQGVDGLLPHMKQDGVQIVFDATSAYVHAENSRKVNEQGALMIDLTP
                     AAIGPFCVPPVNLKEHLGRGEMNVNMVTCGGQATIPMVAAVSRVQPVAYGEIVATVSS
                     KSAGPGTRKNIDEFTRTTAGAIEKVGGARRGKAIIVINPAEPPLIMRDTVHCLTDEEP
                     DQTAITESIHAMLAEVQKYVPGYKLVNGPVFDGKRVSIYLEVEGLGDYLPKYAGNLDI
                     MTAAAARTAEMFAEEMLAGRLTLQAA"
     gene            complement(14466..15248)
                     /gene="dmpE"
                     /locus_tag="PK518_03800"
     CDS             complement(14466..15248)
                     /gene="dmpE"
                     /locus_tag="PK518_03800"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HRM48065.1"
                     /translation="MQTKDIEQLGDALFEALTECTTLAPLSSAHPGMTIDDAYAVQQR
                     LMARRLAAGERVVGKKIGVTSQAVMNMLGVFQPDFGWLTDAMVYNEGQAIPMSRLIQP
                     KAEGEIAFVLKKTLQGPGVTAADVLAATEGVMACFEIVDSRIQDWTIKIQDTVADNAS
                     CGVFVLGDRLVDPRDVDLGTCGMVLEKNGDIVATGAGAAALGHPANAVAWLANTLGAR
                     GIALEAGEVILSGSLGIMVPVQAGDNLRVTIGGVGGCSVRFV"
     gene            complement(15306..16766)
                     /locus_tag="PK518_03805"
     CDS             complement(15306..16766)
                     /locus_tag="PK518_03805"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HRM48066.1"
                     /translation="MKEFLNFINGEYVRNASGKTFANINPVDGSVIGSIHEAGQAEVN
                     AAVAAAQAALKGDWGRLSVVERCKLLDAVALEINRRFDDFLQAEIADTGKPHHLASHL
                     DIPRGAANFQIFIDTIKSAATESFQMRTPDGKTALSYGVRVPRGVIAVVCPWNLPLLL
                     MTWKVGPALACGNTVVVKPSEETPATATLLGEVMNQVGMPKGVYNVVHGFGPDSAGEF
                     LTRHQGVNGITFTGETRTGEAIMRAAASGVRPVSLEMGGKNAAVVFADCDFQVAVDTL
                     TRSCFENAGQVCLGTERVYVERPIFEKLVTALKERAETMTPGHPEDKATKIGPVISKE
                     HQQKVLGLYAKAREEGATIVTGGGVPDMPAHLKGGSWVQPTIWTGLSETATVVTEEIF
                     GPCCHISPFDSEDEVLAKVNANRYGLATSVFTQDISRANRLAQQIEVGLCWINSWFLR
                     DLRTPFGGSKQSGIGREGGVHSLEFYTELRNVMVKF"
     gene            complement(16848..17741)
                     /locus_tag="PK518_03810"
     CDS             complement(16848..17741)
                     /locus_tag="PK518_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRM48067.1"
                     /translation="MKKSHIAAAALAALGSLFATQALADGHYVPGVEGMQAASVPPPG
                     MYYLGYLVNYQIDDFRAPGSSSNLPGHNRGTVTALANRFVWVTNHKLLGADYGVEAIV
                     PVMRTSLTINAAGISDSRSGVGDVYLGPLVLGWHGPQWDAVAAAGMWFDTASTSAPAS
                     PGKGFKSTMLTGGLTYYFDGAKTVSGAALMRYEFNGRNSAGMRPGDQLTLEWGLGKSF
                     GAVSAGLVGYSQWQTTNDSGAGASANKAARHAVGAELVYPIPGAGVFLKGALYKEVSA
                     KAGTGAQPKGSLLRFTLVKAF"
     gene            complement(17755..18183)
                     /locus_tag="PK518_03815"
     CDS             complement(17755..18183)
                     /locus_tag="PK518_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRM48068.1"
                     /translation="MAHSVEQRAITAEAANAAVLAAVAKAGELGIRISAAVTDASGVL
                     AAFLRMPGAFLHSVDIAVDKAYTAAGFGFATSQWAGILQGDEALRLGMPVRPRNVVFG
                     GGLPMREGGVLIGGIGVSGGSAEQDEICARAALVALGLDN"
     gene            complement(18185..19111)
                     /locus_tag="PK518_03820"
     CDS             complement(18185..19111)
                     /locus_tag="PK518_03820"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HRM48069.1"
                     /translation="MAMTGVLRPGHAQIRVLDIEESVKWYTDVMGLKYQGRDSQGRAY
                     FKTRAERDHNSIILRQSDRAGMDFFGYKVLDKATLASLEKQLNEYGVKTERIPAGELL
                     ETGERVRFQIPTGHMIELYADKTAVGSAVGNSSPDMDIIDQPGITPIRLDHALIYGTD
                     LDGATDLFTKVLGFTLVERVKLEDGQTDLGVWLTCSAKAHDIAMVRHGEPGKLHHVSF
                     QLGSWEQVLRAADIMSKNRVSIDIGPTRHGITNGTTIYFFDPSGNRLETFAGGYDHYP
                     DMEPITWSWEEVGRGIFYHDRKLNDAFLSVVT"
     gene            complement(19132..19479)
                     /locus_tag="PK518_03825"
     CDS             complement(19132..19479)
                     /locus_tag="PK518_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HRM48070.1"
                     /translation="MGQYYTVTIAETQESYRCLDERSVLQGMEALGKKGIPVGCRQGG
                     CGVCKVHVLEGSYDKRVMSRAHISAEEEAEGCVLSCRIKPTSDLRLSVVGSMKKTVCR
                     SPSTTDASLSQPT"
     gene            complement(19492..20568)
                     /locus_tag="PK518_03830"
     CDS             complement(19492..20568)
                     /locus_tag="PK518_03830"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenol 2-monooxygenase domain-containing
                     protein"
                     /protein_id="HRM48071.1"
                     /translation="MSHQLTIEPLGQTIEVEEGQSILDAALRAGIYLPHACCHGLCAT
                     CKIQVTDGEIEHGEASGFALMDYEREEGKALACCATLQSDVVIEAEIDEEPDARNLLV
                     HDYRATVVRIEDLTPTIKGVWLKLDQPMEFQAGQYINLHIPGEAAPRAFSIANAPTAA
                     GGRDEIELNVRHVPGGKGTTWVHQQLKSGDAVQLSGPYGRFFVRESAHAKEGLGYLFL
                     AGGSGLSSPRSMVLDLLAAGCSKPITLVNGARSQDELYHHAEFEQLADKHPNFSYVAA
                     LSGEAEDSGWTGARGFVHDAAKAHFANDFRGHKAYLCGPPLMIDACISTLMQGRLFER
                     DIYTEKFISAADAQQVRSPLFKKI"
     gene            complement(20663..21019)
                     /locus_tag="PK518_03835"
     CDS             complement(20663..21019)
                     /locus_tag="PK518_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit P4"
                     /protein_id="HRM48072.1"
                     /translation="MAVKSIAPYEFEQKDTVDKFPAPLLYIGWEDHKMFCAPFCAPMP
                     PTMKFGDLVNGALPGMYGAHPDFARIDWDQAEWFMSGKPFTPDMDKTLAEHGLGHKSV
                     IRFRTPGLTGLSGSCF"
     gene            complement(21053..22600)
                     /locus_tag="PK518_03840"
     CDS             complement(21053..22600)
                     /locus_tag="PK518_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene/methane monooxygenase
                     hydroxylase/oxygenase subunit alpha"
                     /protein_id="HRM48073.1"
                     /translation="MDMKATKKLGLKERYNLMTRDLAWNPSYQAVKDVFPYMEYEGIK
                     IHDWDKFEDPFRMTMDSYWKYQAEKERKLYAIIDAFTQNNGHLGVTDGRYINTLKLFL
                     TGVSPLEYMAHRGFAHVGRQYPGAGPRVACMMQSLDEIRHAQTQIHSLSNYNKHYNGF
                     ADWRHQHDRVWYLSVPKSFFDDAVSAGPFEFIVAIGFAFEYVLTNLLFVPFISGAAYN
                     GDMGAMAFGFSAQSDESRHMTLGLEIIKFILEQDPDNLPIVQAWLDKWFWRGYRVLTL
                     VAMMMDYMLPKRVMSWKEAWEIYAEQNGAALFNDLARYGIKVPKGWEQACKDKDHLSH
                     QAWNVFYNYGAAAPFHTWAPSEKDMDWLSAKYPETFDKYYRPLWQHYAKEQAEGKRFY
                     NGTLPMLCQVCQVPMFFTEPDDPTKVAYRESEYKGMKYHCCSDGCKHIFDNEPEKYAQ
                     SWLPVHQIYQGNCFPEDVDPTQPDFNPLAEVLRWYKIRQGEDNMDYADSPDKKNFENW
                     RSQATGN"
     gene            complement(22649..22918)
                     /locus_tag="PK518_03845"
     CDS             complement(22649..22918)
                     /locus_tag="PK518_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HRM48074.1"
                     /translation="MSNVFIAFQKNEDSRAIVNAILADNPGAIVSDQPAMVKIDVPGH
                     LVVRKSTIEEEIGRAFDLQEMQVHLITMSGNVDETDDEFTLTWHQ"
     gene            complement(22952..23947)
                     /locus_tag="PK518_03850"
     CDS             complement(22952..23947)
                     /locus_tag="PK518_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HRM48075.1"
                     /translation="MQIDLRTVSITPLRQTYSHIAERLGADKPASRYIEGTMNVQPDA
                     NFHYRPTWDPEHELFDPRRTRLVMRDWYALKDPRQFYYGAYTQARARMQEVAEADFEF
                     VEERGLADRYDDAARRKALDFYVPLRHVAWGANMNGAYQCAYGYGTAITQPCLYAGMD
                     QLGIAQYLTRLGLLLGNQGELEAGKQAWLQAPAWQGLRRLVEDTWVLKDWFELFVAQN
                     LVLDGVLFALAYKEVDQVLSEQAGPVVSMLTRFQAEWGQDANKWVDAVMKVAAAESPE
                     NKELLSHWYAHWRGRVQQALAPIAELALGADGADALARSVAAVDGRMNKAGLTLA"
     gene            complement(23986..24276)
                     /locus_tag="PK518_03855"
     CDS             complement(23986..24276)
                     /locus_tag="PK518_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit"
                     /protein_id="HRM48076.1"
                     /translation="MSTTPPPSEDRWDVQRRFIRIVQEHDNGMVEFEFAVGEPGLYVE
                     MVMPRQQFEEFCAMQAVVPTHGRLPEHGEDSAEHEWDWSLRDARERHFRQEP"
     gene            24526..26226
                     /locus_tag="PK518_03860"
     CDS             24526..26226
                     /locus_tag="PK518_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRM48077.1"
                     /translation="MPTLPALPSDADLRRQIQFSAADGRIWLAGQRMVLMHAASLGIL
                     RRELMHTLGPTAARRLLLRVGYASGERDAALARQLRPGADAFAMFSVGPQLHMLQGAV
                     QVAPRRLELDMASGHFHGIFDWQHSWEVETHVRDFGAQHNAVCWTLLGYASGYTSAFM
                     GRTVLYKEVQCAACGHAHCRIEGRPVQDWPDGAQVASDYAPDALALPEGARTPVAWPP
                     PVPAEDRPEPPADALGQLIGRSTGFNAATQLLRKAASTQVTVLLTGETGVGKERFARA
                     LHALSPRAAKPFVAVNCAALPGSLVESELFGTEKGAYTGADAARAGRFERAHGGTLFL
                     DELGELPLPAQAKLLRVLQDGQVERLGATQPRQVDVRLVAATHQDLAVAVRAGRFRQD
                     LYYRLNVYPIRIPPLRERLDDIEPFARHLLERFAARHDKHIAGLSDRALHALRQYAWP
                     GNMREMENLIERGVILADPGQPVDAAHLFPDGTGAASGTPTLTLQASGRLGETRASGC
                     DDTLIARLLGDGLDLQALETQLMEAAVQRSHGNLAAAARLLGLTRPQLSYRLNKPRES
                     "
     gene            26242..26976
                     /locus_tag="PK518_03865"
     CDS             26242..26976
                     /locus_tag="PK518_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM48078.1"
                     /translation="MAKRSALGAIDDPAPSGRTLIEQAYARLRDDIVTGQLGPGERLR
                     VEHLKTDYGVSAGTLREAITRLASDALVVTEGQRGFRVAPIALEDLEDITNLRVQIET
                     MALRQSIRAGDGLWRQRVEQAFAAISAEEPIAPERRRHWENLNVDFHEALLSGYASAW
                     TLRVLRLLSRHTERYRSFAMALPGAVRDVHAEHTAIVEFALSGQDARAALALEAHIRT
                     TPDLLMQALREGRASLPGNGAPPPPP"
     gene            complement(27024..>27115)
                     /locus_tag="PK518_03870"
     CDS             complement(27024..>27115)
                     /locus_tag="PK518_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HRM48079.1"
                     /translation="STHPTGPDRIRELEQNVPRVQGLYLQSRR"
BASE COUNT         4121 a         9097 c         9216 g         4681 t
ORIGIN      
        1 gccactcggc ctcggagcac acgaccttgg cgcggctgga ttcaaagttg catttctcgc
       61 cctgatccag cagccaggcc ttgtgccaga tatgcaggcg cgcggtgtgc agatccatgt
      121 cgttgtcggc cagcatgaag cccaccccct cgtgctcggc cagcggccgg ccgaaggcct
      181 ggcgccggcg cgcgtaggcc agggcctggt cgtgcgcacg ccgggcctgg cccagccagc
      241 gcatgcagtg cgtcaggcgc gccggggcca ggcgcacctg ggcgtagcga aagccctggc
      301 cgacctcgcc cagcacatcc gttgccggca cacgcagatc gtcaaagcgc agcacgccgt
      361 ggccgccggc aaagcagctg tccatggcgt ccatgttgcg ctccagggtg atgccgggcc
      421 ggtccatgtc ggacaaaaac atggtcgccg agccgtcctc catgcgcgcc atgatgatgg
      481 cgtaatgcgc cccttcggcg ccggtgatga accacttgcg gccgttgatg acgtagtcgt
      541 cgccgtcgcg ccgggccgtg gtcgccagca tcgacggatc agccccggcg ccgggcgcgg
      601 gctcggtcat gacaaagcag gagcgtgtcg cgcctgccac ctgcgggcgc agccagcgct
      661 ctttttgcac gggcgtcgcc accgcctcca tcaaatggat gttgccctcg tcgggtgcgt
      721 ggatgttgag cgccgtcggg ccgagccagg agtagccggc ctcctcgaag accacggcct
      781 tttccacatg gctcaggccc aggccgccca tctcgggcga cgcgtgcggc gtgagcagcc
      841 ccgccgccct ggcgcgcgcc accagctcgt cgcgcaacgc ctcgctgggg ccatggtgtg
      901 actggcgctc gtcgttttcc agcggaatca cctgttcggc gacgaattgc ctggttttgt
      961 cgcgcaatgc gcgcagttca gggggcaaag agaaattcat gcgctacctc gtgataaggc
     1021 ccggcagggt gaccggcata ggaagctttc taccgcactc cgccgccacc gaatttcagt
     1081 gacgcgccgc ccggtgcaag ggcaagccag gcacccccag cgtggcgcgc aggatgcgcc
     1141 catgcgtgga gtcggtcacg tagatctgac cccggtccgc gccgccgaag gccaggttgg
     1201 tgatggagct gcccggcaga ccgcgcatca cctgcaccgg ctccgcgcgg tcgttgagca
     1261 cccagacata gcccaggccc ggattggcga cgagcaggcg gccctcggcg tccacggcca
     1321 ggccgtcggg gccgctgggg ccgtaggagg tgaagaactg gcccaccttg gccacgctgc
     1381 cgtcgggcag cagcggcacg cgccatacgc agttgccgcg cgtcacggcc aggtacagca
     1441 cgcgctcgtc gggcgacagc gccacgccat tgggactggg cacgttggtg agcaacaggt
     1501 cgagctggcc gttggtgcgc aggcggtaca ggcgcccgct ggggtcgtgc agcccggtct
     1561 gcccctggtc ggtgaagtac aggttgccct ggctgtcgaa caccaggtcg ttcacgccct
     1621 tgaagcgttc gctgttgcgc cgctccagaa aagggctgac ctggccgctg gccagatcca
     1681 ggcgcatcag gccgttcttg tagtcggtga tgagcagcgt gccggcatcg aggaacttca
     1741 tgccgttggg ctcgccgtcg tattcggccg ccagcgtcca ggcgccctgc gggtcgatgc
     1801 ggaaaacgcg gccccagggg atgtcggtca cgtagaggtt gccgtcatcg ccccacaccg
     1861 ggccttcgag gaaggagtcc gtcaccacgc cgccgcggtt ggcatcggcc cagtcgctgc
     1921 gctcgcggcg gcgcagcggc tcgggcatgg aggtgaacgg ctccaattca cacacctggg
     1981 gggattgcag cagaaacatg aaaaattcct ttcaaaagcc gtagagccgc gccgggttgt
     2041 ccacgaggat gcggtccatg gccgcgtccg acccggccca ggcccgcagc aggtccacca
     2101 ggtcgacatc gttcaccgtg cccggcgccg cggtggtgtg cggccagtcg ctgccccaca
     2161 ccagacgctc gggcgcggcc cgcaccagcg cctgagccag caccagcgta tccgcatatg
     2221 cgggcgcggt cgagcgcatg tacgcgcccg agagcttgac ccaggtgtta ccgccgtcca
     2281 gcagccggcg cagcgcgccg tagccgtcgg cggacggccc ctcgtccgga tcgatgcggc
     2341 ccaggtggtc gatcaccagc ggcacgggca gcgcggccag cacgctttcc agggccacga
     2401 tctgctccgg gtgcatgaag acctggatgt gccagagcgg caggcgcgcg gccttgcgcg
     2461 ccagcgtggc cagcatctgc ggcgtggtct cgccccagga ttgcggcgtg acgaagttca
     2521 cgcgcagccc gcgcacgcgg cgcgcgtgca ggcggtcgag ttcggcatcg cccacgtcct
     2581 gcgccaccac ggccacgccg cgcgcgccgt cgcccagctg gtccagcgca tcgagcgtgc
     2641 aggcgttgtc ggtgccgtag gtagaaggcg tgacgaccac cgtgcgctgc gtgcccagtc
     2701 gctgctgcag ccgccggtag gcggccacgg gcgcatctgg cggcgtgcgc ttccagtgcg
     2761 gcgacggtgc gaaacgcgga tcgaagatgt gcatgtggct gtcgcaggcc aacggcggca
     2821 gccggcgcgc gggccggttc aggcccacgg aatgcggcac ggtgtgcgca gtcattgctt
     2881 gtcgatgccg accttggccg ccacggcctc ccacagctga gcatcgctct tgagcttgtc
     2941 ggcgaacgcc tggccgctca ccacgccggg caccgcgccc aggtcgttgg cgaacgactg
     3001 cacgtcggcg ctggccatgg ccttggcgat gttcttgcgc agcgtctcca gcaccgcctc
     3061 gggcgtgccc ttgggcgccc acaggccagt aaagttgacg accttgtagt cgggcgcacc
     3121 cgcctgcgcg aaggtgggca cgtcgggcag cgccttcagg cgctgctcgc cgctcaccgc
     3181 cagcaggcgc accttgccgc cgcgcacctg gcccatgacg ccgggggtgg aggcgatctg
     3241 gaagtcgatg gtgccgctca gcagcgccag cgtggcctcg cccgcgccct tgaacggcac
     3301 atgggtggcc ctcaggccgc tgacgccttc cagcgccacg gtggcgaagt gcggcatggt
     3361 gcccgcgccg ccggtgccgt agttcagctt gcccgggtgg gcctgcgaat acgccaccat
     3421 gtcgcccagc gtcttgaacg gcgagttggc ggccaccacc actgtcgtgg gcgcgaagtt
     3481 gaagccggcc acgggcacca ggtcgtgcgc gtaatcccac ggcagcttct tgaagacctg
     3541 cggcaggatg gagtacgtgg tgtcgttggc cgtgagcgtg tagccgtcac ccggggagcg
     3601 cgccacctgc gccgcgccga tggtgccggt ggcgccggcc ttgttctcga cgaagaaact
     3661 ctggccggtc tgctcggtca gcttttgcgc gatgcggcgc gtcacctggt ccacggcgcc
     3721 gccgggtggg tacggcacga cgatcttgac gggcttgtcc gggtaggccg tttgcgcctg
     3781 cacgccagcg gcggcgcagg ccagcgccag ggcggcgcac agagggaagt tcatacggtt
     3841 catgggtgtt gtctccttgt ggggctcatt cggccttcag gttcagatcc ctggcgatct
     3901 gcatataggt ggcgacttcg cgctcgacct gctgcgcgaa cgccaggccg caggtggagt
     3961 cggcatccag ccccaggccg cgcagcttct cggcggcggc ggtgctggcg gcgaagtcgc
     4021 gcgccgcctt ctccagttgc tgcgcgatgg gctggggcag cccggccggg gccagcacgc
     4081 cgtaccaggc gtcggccgaa tagttgccgc cgcccgcttc ggcgaaggtg ggcacgccgg
     4141 gcagcagcgg cgagcgcttg ggcgacgcca cggccagggc cgcgagcttg ccctctcgca
     4201 gttgcggcag caccgagccc agcgtggcga aggagctgtc gagctgggcg cccatcaggt
     4261 ccgtgaccac gggcgcggcg cccttgtacg gcacgtggtt cagctgcagg ccgttggcac
     4321 gggcaaacat ctcggtggcg aagtgacccg agctgcccac gcccgccgtg cccgcggtgg
     4381 tgcggcgggg ctccttgcgc gcgcgggcca tgtagtcggc cagcgatttc accggcatgc
     4441 ccggccccac caccaacacc gtcgggctgg tagcgacggt gcacagcggc tggaaggact
     4501 tcaccgcgtc gaacttcacc ctggtgccgt agagcgcggg aatcatggtg tggttggtgg
     4561 cgccgaacag cagcgtgtag ccgtcggccg gcgcgctggc cacggcctgc gcggccagga
     4621 tggtgttggc gccgggcttg ttctccacca cgaagggctg gcccagcgcg cgggctgcat
     4681 ggtcggcaaa ggcgcgggcc accacgtcgg tggggccgcc ggcggcgaag cccaccacca
     4741 gcttcacggg cttggagggg tagcccgcct gcgcgaagca ggcgccggat gctactaata
     4801 aaatagctgt aagcgctttt tcagcaagcg ccagaagccg attttgcttc agcattttga
     4861 gtctccttgc agtcaatcca tgcgggggcg cgggcgcgga ggccgcacca acgcgcctac
     4921 tccttcttga tgcctgcggc cttgatggtc tcggcccacc gcgcggactc cgccgcgagg
     4981 cgcttgcgga actcatccgg ctgcgagccc acgatctctg agcccagcgc gtcgaagcgg
     5041 gccttgacct cgggcatggc cagcacttcg cgcaccgcgg tgttgagccg cgtcacgatc
     5101 tcagcgggcg tggtgctggg cacgaacatg ccgttccact cgtagacctc gtagttcttc
     5161 acaccggctt cgctgatggt gggaacgttg gggaacaagg ggttgcgctt ggccgaggtg
     5221 atggccaggg gcttgagcgc cccgcccttg atgtggctgc tgagcgccgg caggttgaag
     5281 aaacccatgt ccacttcacc cgcaagaatc gcctgcagcg ccagtccgcc agcgcgatac
     5341 ggcacgtgca gcatcttgat gcccacgcct tggttgaaca gctcggcgcc catgtactgc
     5401 acggtgccct ggccgcccga gcagaaggtc agcttgccgg acgccttggc ggctgccgcg
     5461 atcaactcct gcagcgtgtt gtagggcgaa ttcttgggca ccacgaccag catgggcgtc
     5521 acgcccacca gcgccaccgg ctgcaggtct ttggcggcat cgaaaggtag cttctgcaga
     5581 tgggggttga tggacagcgg cgtcgcgtcg tacagcaccg tgtagccgtc cgcaggcgcc
     5641 tgcgccaccg atgccgcgcc gatgacgccg ctagcgcccg ccttgttcag aacgacgacg
     5701 gacaccttga gccgtgtccc caggtgctgg gcaagcaccc gggccagggc atcggccgtt
     5761 ccgcccggcg cgtagggcac caccagcgtg atcggcttgt tcggccaggc cgattgtgcg
     5821 gtggcgggaa tcgcggtgaa cgccgccagc gcagcggcca gcacgttgaa attgcgacgg
     5881 ttcatgacgt gttgtctcct ttggaattga aacgggctgg aatcaggccg tggccgtgcg
     5941 gttcagcacc gccagcgcat tgcgcgccgc acccaggccc atgttcacgt aggcgtcgct
     6001 ggtcacgcca ccgatgtgcg ggctcaggat gaagccgctc tggtgctgga acgggtggcc
     6061 tgcggccatg ggctcgacgg cgaaactgtc cagcccggcc atgcgcacct ggccgctgtg
     6121 cacggcggca agcagtgctg cctcgtcgat caggccgccg cgcgcggtgt tcaccacgat
     6181 cacgcccttt ttgcagcgcg ccagcgtgtc ggcgtccagc aggtcacggt tgtcatcggt
     6241 caacgggcag tgcagcgaaa tcgcgtcgct ctcgcgccag atggtttcca ggtcggcggc
     6301 ctgcacgaag tccggcaggt tcttggcgaa cgggtcgaag ccgagcacgc gcatgcccaa
     6361 ggcgtcggcc atcttcgcga agcgcaggcc gatggcgccc aggccgacga ggccgatggt
     6421 gcggccgccg agttccacgc tcttgtgcgt ggccttgtcc cagtggccgg cgtgcatgcg
     6481 ttcgttgagc tgcggcaccg acttggcgca ggccagcagc agcgccagcg cctgctcggc
     6541 cacggcggcg gcgttggcgc ccacggcggc caccacctcg atgccgcgcg cctgggccgc
     6601 tgccttgtcg atggtgtcgg tgccgctgcc atgcttggag atcaccttga gcgacggcgc
     6661 cgcgtccatg gccgccgcgc ccaccttgct gtagcgcacg atgatggcca cggggttgtg
     6721 ccggcggcac agggcgacta cttcgtcctc cgtgggcgtc ttgccggcat agacgatgtc
     6781 gtaatcggcg agcagcgcca gcgcctgcgg ggcgaggtcg gccgccgtga cgaggatggc
     6841 ggcgcggctc acagcgtttc tccttctttc agcacgccgg cggcgcgcag cgcggcgggc
     6901 agccacttgg cagccgtgtc gccgcgggct attgattcga tgcgcgcggc ctcgtcggcc
     6961 accttcttca cggccagggc cagcatggcg ggcgccttgg cgcgttcgat caccactacg
     7021 ccatcggcat cgcccaccac caggtcgccg gggtgcacca tggcaccgcc gcaggcgatg
     7081 gggtggttca cgcggccggg cacgaacttg gtcggccccg ccgggttgaa gccggcgctg
     7141 aagacgggga agtccagctc cagcagttcc agcttgtcgc ggatgcagcc gtccacgatc
     7201 acgccgccca ggccgcgctt cttgcaggcg ctgagcatca gcgtgcccat gagggccgcc
     7261 gtctggtcgc ccttgccgtc gatcaccagg atgtcgcccg gctgcgccag cgcgatggca
     7321 gcgtggatca tcaggttgtc gccggggcgc acctcgacgg tgaaggccgg gccggcgagc
     7381 cgcatgcgtt catgcaccgg cgccacgcgg gcgtgcatgg tgccgcggcg gcccgccacg
     7441 tcggcgagga tggcggcctg gaaggtggcg gcctgttgca cgacgtcggg gctgacgcgc
     7501 tcgatggtgc ggatcacttc ggggagttgg ttcatggaaa tctcggcagt gaaggtaaaa
     7561 cggtgcgaca caaccgccac cacgcaggat gcaggtggcc gtgccgcatg gaacggatca
     7621 ggaaatgagc gcttcagtcc aagctaacgc ccgaggcctt gacggtcttg gcccagcgcg
     7681 gcagttcggc gcgcagcagc tggtcgaact tctcggtgct gccgcccagc acctcgccac
     7741 cctctgagcg cagcttctcg gccacgtcgg gttgctgcag cgccaggttg atggccttgt
     7801 tcaactgctg cacgatggcc gcaggggtgc cggcaggcgc cagcaggccg aaccaggtga
     7861 cggactcgaa acccttgtaa cccgattcct ccagcgtggg ggtgccgggc agctgatccg
     7921 agcgcttggc cgaggtcagc acgatggcgc gcagcttgcc gttgcgcacc tggcccagca
     7981 gcgtgggaac ggaggacatg tacaggtcga tctggccgcc caccaggtcc gtcatggcct
     8041 gggccgcgcc cttgtagggc acatggcgca gctggatgcc ggcggcgttc tcgaccaact
     8101 cgcccgacag gtgggccacc gtgccgttgc cggaatagcc cagcgtgatg ccgtcggggt
     8161 tggccttggc ggcggccacg acgtcggcaa agctcttgta gggcgagccc gccggcgcgg
     8221 ccatgacgat gggcgaggac gacagcagcg ccaccggcac caggtccttc agcgggttgt
     8281 agggcagctt ggggtagagc gaggggttga tcgccaggtt gctggtctgg cccacgacca
     8341 gggtgtagcc atcgggggct gccttggccg ccgcgtccac gcccagattg ccgccggcgc
     8401 cggggcggtt gtccacgacg atggtccagc gattctggtc ggtgagcttt tgcgccaccg
     8461 tgcgcgcgat catgtccgtg ccgccacccg gggggaaggg cacgatcagg cggatgggct
     8521 tggccgggta ggcggtctgc gccatcgcgc cggtcgcggg cacggcggcc gcgagcaggc
     8581 caagaagcgt cgcacccagg gatgcggtca gggaaaagcg tcgagagaag gccatgggtt
     8641 tgtctccgtg tatgtcatga accgctgccg gcacacaggc gcaggcatcg ggacgcaact
     8701 ttaagaaagc gtttcaacct atacaataga gttgcgcaca atgaaacata tcccacggta
     8761 agcaacatga gtcgtcccgc taatcccccc ctctccgccg atgaatcaac gcctgcgggc
     8821 gcacgcgacg gtggcgtcat tgccgtgacg cgcgcgctgc ggctgctcga gtccttcgcc
     8881 gtgggcgagt cccagctgtc gctggccgag ataagccggc gctgccagct gcacaagacc
     8941 acggtgctgc gcctggcacg cacgctggcg cagtcgggct acctggtgca gcgcgaggac
     9001 ggcgactggc gcctgggccc ggccgcgggc tggctgggag cgcgctacca ggccggcttt
     9061 gacgtgcaga acgtgctgga gccggtgctg cgcgagctca ccctggccac cggcgaaagc
     9121 gcggcgttct atgtgcgcga gggcgacatg cgcacctgcc tggtgcgcgt ggaaggcccg
     9181 caggcactgc gccaccatgc gcgcatgggc gaaggtctgc cgctagacaa gggctcgccg
     9241 ggccgcgtga tcctggcgtt ctcgggcgag ccgggcgagg tctacgagga gatccgccgg
     9301 cgcggctacc actggtcgat cggcgagcgc gagcagggcg tggccacggt gtccgctccc
     9361 gtgttcggcc gccactggcg cctgctgggc tgcgtgtgca tctcggggcc acagtcacgg
     9421 ctgccgcagg agaagctgga ggcgctggcc cagaccatca tcaggacggc caaccagctg
     9481 tcctacgcgc tggccggcaa ggccgccagc acggcggcgc aggcgccggg cgtctggcat
     9541 ccgtaaggcg acgcccagcc agccgataca ctcgcccggc catttgcctc cgaccttgcc
     9601 cccatgctcc actacctgac cattcccgtc accgccttcc agcaaaacag ctccctcgtc
     9661 tggtgcgacc agaccatgga ggcggccgtc atcgacccgg gcggcgacct cgatctgctg
     9721 ctggccgaag cgcagcgcct gggcctgcac ctgaaggcca tctggctcac gcacgcacac
     9781 atcgaccacg ccggcggcac gggcgagctg gcgcaaaagc tcggcctgcc catcatcggc
     9841 ccgcatccgg gcgaccagtt ctggatcgat ggcctacccc agcaaagcca gatgttcggc
     9901 tttccgccgg ccctgcattt cacgcccacg cgctggctgc acgatggcga tacggtcacc
     9961 attggccacg agacactgaa cgtgcgccac tgcccgggcc acacgcccgg ccatgtggtg
    10021 ttccacgcgc cgcagatcga ccgctgcttt gtcggcgacg tgctgtttgc cggcagcatt
    10081 ggccgcaccg actttcctca gggcaaccac cagcagctga ttgacagcat caccggccgc
    10141 ctgtggccca tgggcgacca gacggtgttc atccccggcc atgggccgga gagcacgttc
    10201 ggacgggagc ggcgcagcaa cccctatgtc ggtgatacct gagcgattta ctacaaatta
    10261 aatagcttat ggcgctttcc tataaagcgc tacaagccgt tttgccacat atccctgtca
    10321 gcgccccagc tccttggcgc tgaccccggc gatgccccag ttctctttgg gcacgtcgtg
    10381 gatccagacg cgcacattgg ccacgggtgc gcccacggca tcgacgaggg cctgtgtcac
    10441 cttctcgatg acggccttct tctgctcctc ggtgcggcct tcaagcatgt agatctgtgc
    10501 aaatggcatg gtgctccacc ctggtaagaa aagaggtcaa tcggccttga ggccgagctg
    10561 gcggatcagc ccgccccagc gccggttctc ggcgcggatg aaatcggcga actgctgcgg
    10621 cgtgttcaca cgcaggttgc ccgagatgtt gtcgaaggcg gcctgggcct gtggcgtcct
    10681 ggcgccggcc tcccaggcgt cgtgcagctg ggccacgacg gccggcggcg tgccggctgg
    10741 cgccaccagg ccaaaccagg tctcgacctc gaagccgggg taacccgact cggccacggt
    10801 gggcacgtcg ggcagggcct tggcgcgctg cggcgaggtg acggcgatgg ccttgagctt
    10861 gcccgcgcgc acatgctgca gcgtggtggc cagcgagctt tccatgctca ttggcaccgt
    10921 gcccgccagc agatccggca cgatctggct ggagcccttg tacggcacgt tggtgagcgc
    10981 tatgcccgcc accgactgca gcagctcggc cgccagatgg ttgctggtgc ctatgcccga
    11041 ggtggcgtag ctcagccggc ctgggttcgc cttggcatgg gccaccatgc ccgccaggtc
    11101 ggagaacggc gcccccgggt ggatgaccag ggcgcagggc gtggtgccca ccagcgagat
    11161 cggcagcaag tccttctcca cattgaaggg caggctggcg tacaggtgcg gcgcgatcgc
    11221 caggctgctg atggccgcca ggcctatggt gtagccgtcg ggcgcggcct tggcgacgaa
    11281 gtcggtgccg atgttgccgc ccgcgccggc cttgttctcg accacgaact gctggcccag
    11341 ccgctcgccc atggccttgg ccgccgtgcg gcccatcaca tcggtcggcc cgcccggcgg
    11401 gaagggcacc accatgcgca ccggcctggc cgggtagcca ctctgggccc aggcgccgcc
    11461 ggccaagctg gcgccgcatg cggccagcgc gccggccgtg atgaagtcgc gtcgttgcat
    11521 ggtcttgtct ctggttgttg ttatgaaaag gctcaggcaa agcgcagcga cacgctgccc
    11581 atgccctgca cgcgcaggct gacgttgtcg ccggcctgca cggccacggc ctcggtgacg
    11641 ccgcccgaca ggatcatgct gcccgcgggc agctcgcgcc cgcgccggcc caggtggttg
    11701 gccagcatgg caatggccgc ggccgggtgg cccagcacgg cggcgccggc gccaagggcc
    11761 acgggctcgc cgtttttctc cagcacgatg ccgcaggtgc gcaggtccac gctctcgggc
    11821 cgctgcgcct ggccgccaat gacgaagcgc gcggccgagg tgttgtcggc caccacgctc
    11881 ttcaagtcga acttgaagtc gcggtagcgg ctgtctatca cctcgatgcc cggcagcacg
    11941 aagtcggtgg cggccagcac ggcgccgata tggcagcccg gccccttcag cgcatgcttg
    12001 agcacgaaga cgatctcggg ctcgaccttg gggtggatga gctcgcgcgt ctgcaccgtg
    12061 ccgccctcgg gcacgctgta gtcgtccacc agaaagccga acaccgggtc ggtcacgccc
    12121 atctgcttca tcttggcgtg cgaggtcagg cctgccttca gtcccaccac gcgcgcgcca
    12181 cgcgcgagct tgcgcgccag gatggcgtcc tggatggcgt aggcgtcatc ccagtccatg
    12241 tccgggtgct cgtcggtgat cttgggcgtg tcacgcacct gcagctggca ggcctccagg
    12301 tgctcggcca ggcgctctat ggtgtctttg ctcagtgcca tgttgtgttc ctttcttcag
    12361 gcggcaacgg ccaggccgcg cgccctggcc atggtgatgg ccgtgtcctc gatcatgtcc
    12421 tcctgcccgc cgaccatgcc gcggcggccc agctccagca ggatctcgcg cgccggcacg
    12481 ccgtatttct gctcggcacg cttggcaaac agcaggaagc tgccgtacac gccggcgtag
    12541 cccagcgtca gcgcgtcgcg gtcgatgcgg atcgggaagt ccatcagcgg cacgaccaga
    12601 tcctcagcca cgtcctggat cttccagaca tgaacgcccg tggggatgcc catgcggtcg
    12661 agcacggcca ccagcacctc catgggcgtg ttgcccgcgc ccgcgcccag gccggctgct
    12721 gccgcgtcga tgcgattggc accggcctct atggcggcaa tgctgttggc cacgcccatg
    12781 gccaggttgt ggtggccatg gaagcccagc tctgtttcag gcttcaaggc ctgacgcacc
    12841 gcgctgatgc gctcggtcac atgcccgggc agcatgtagc cggccgagtc ggtgatgtag
    12901 atgcaattgg cgccgtagcc ttccatcagc ttggcttgtt tgaccagacc ggcggcgtcg
    12961 ttcatgtgcg ccatcatcaa gaagcccacg gtgtccatgc ccagcttgcg tgcctgggtg
    13021 atgtgctgct cgctcacatc gccctcggtg cagtgtgtgg ccacgcggat ggtgtgcacg
    13081 cccagctcat gggccatctt caggtgatcg acggtgccaa tgcccggcag cagcagcgcc
    13141 gagaccttgg cctgcttcat cagcgggatc acggcgccca ggtattcctc gtcgctgtgc
    13201 gccggaaagc catagttgac cgaggcgcca cccaggccgt cgccgtgggt gacctcgatc
    13261 agcggcatgc cggcggcatc cagcccctgg gcgacggatt tcatctgctc cagggtcatg
    13321 aggtggcgct tggggtgcat gccgtcgcgc agcgtcatgt cgtgcagggt gattttctgt
    13381 gtcatggtga tagtccttat gtatgtcgca ccgggcattc actccctctc ccgctggcgg
    13441 gagagggcgg gggtgagggt ggttcttcgt acaggcgcag cgcctgtgca cccccctcac
    13501 ccctgccctc tcccccaggg gggagaggga gtcaagccct tcaggcggcc tgcagggtga
    13561 ggcgcccggc cagcatttcc tcggcaaaca tctcggccgt gcgcgcggct gctgccgtca
    13621 tgatgtccag gttgccggcg tacttgggca ggtagtcgcc caggccctcg acctccaggt
    13681 agatggagac gcgcttgccg tcgaacaccg ggccgttgac cagcttgtaa ccgggcacgt
    13741 atttctgcac ctcggccagc attgcgtgaa tgctttcggt gatcgccgtc tggtcgggtt
    13801 cttcgtcggt caggcagtgc acggtgtcgc gcatgatcag cggcggctcg gccgggttaa
    13861 tgacgatgat ggccttgccg cgcctggcgc cgcccacctt ctcgatggcg cctgcggtgg
    13921 tgcgggtgaa ttcgtcgatg ttcttcctgg tgccggggcc ggcggatttg ctcgacacgg
    13981 tggcgacgat ctcgccgtag gccaccggct gcacgcgcga gacggccgcg accatgggga
    14041 tggtggcctg gccgccgcag gtgaccatgt tgacgttcat ctcgccacgc cccaggtgtt
    14101 ccttcaaatt gaccgggggc acgcagaagg ggccgatggc ggcgggcgtc aggtcgatca
    14161 tcagcgcgcc ctgttcgttg accttgcgcg agttctcggc gtgcacgtag gcgctggtgg
    14221 cgtcaaagac gatttgcacg ccatcctgct tcatgtgcgg cagcaggccg tcgacgccct
    14281 gatcggtagt cttgatgccc atttcgctcg cgcgcttcaa gccgtcggac tccgggtcga
    14341 tgcccaccat ccacaccggc tccaagacgg ggctcctctg cagcttggcc agcaggtcgg
    14401 tgccgatgtt gcccgggcct atcagggcgc atttgatctt gtcactcatc acttttctcc
    14461 acaaatcaaa caaaacgaac gctgcagccg ccgacgccgc caatcgtcac gcgcaggttg
    14521 tcgcccgcct gcaccggcac catgatgccc agcgagcccg acaggatgac ctcgccggct
    14581 tcgagcgcga tgccgcgcgc gcccagggtg ttggccagcc aggccacggc gttggccggg
    14641 tggcccaggg cggcggcgcc ggcgccggtg gcgacgatgt cgccgttctt ctccagcacc
    14701 atgccgcagg tgcccagatc cacatcgcgc gggtccacca gccggtcgcc cagcacgaac
    14761 acgccgcagc tggcgttgtc ggccacggtg tcctggatct tgatcgtcca gtcctggatg
    14821 cgcgagtcga cgatctcgaa acaggccatc acgccctcgg tggcggcgag cacgtcggcg
    14881 gcggtgacgc ccgggccttg cagggttttc ttgagcacaa aggcgatctc gccctcggcc
    14941 ttgggctgga tcaggcggct catgggaatg gcctggccct cgttgtagac catggcgtcg
    15001 gtcagccagc caaagtcggg ctggaacacg cccagcatgt tcatcaccgc ctggctggtc
    15061 acgccgatct tcttgccgac cacgcgctcg ccggcggcaa ggcggcgcgc catcaggcgc
    15121 tgctgcacgg cataggcgtc gtcgatggtc atgcccgggt gcgcgctgct caagggcgcc
    15181 agcgtggtgc actcagtcag cgcctcaaac agcgcgtcgc ccagttgttc gatgtccttg
    15241 gtttgcatta ctacactttc aatagctgtt tgcgcttgac tggcaagcgc tacaagcctg
    15301 ttttgttaaa acttcaccat cacgttgcgc agctcggtat agaactccag cgagtgcacg
    15361 ccaccctcgc ggcctatgcc cgattgcttg gagccgccga acggcgtgcg caggtcgcgc
    15421 agaaaccagg agttgatcca gcacagcccg acctcgatct gctgcgccag gcggttggcg
    15481 cgcgagatgt cctgggtgaa gaccgaggtc gccagaccat agcggttggc gttgaccttg
    15541 gccagcacct cgtcctcgct gtcgaacggg ctgatgtggc agcaggggcc gaaaatttcc
    15601 tcggtgacca cggtggccgt ctcgctcaat ccggtccaga tggtgggctg cacccaggag
    15661 ccgcccttga gatgcgcggg catgtcgggc acgccgccgc cggtgactat ggtggcgccc
    15721 tcctccctgg ccttggcgta caggcccagc accttctgct ggtgctcctt gctgatcacc
    15781 gggccgatct tcgtcgcctt gtcctcgggg tggccggggg tcatggtttc ggcgcgctcc
    15841 ttgagcgcgg tgaccagctt ttcaaagatg gggcgctcga catacacgcg ctcggtgccc
    15901 aggcagacct ggccggcgtt ctcgaagcag gagcgcgtga gggtatccac ggccacctgg
    15961 aagtcgcagt cggcaaacac cacggccgca ttcttgccac ccatctccag cgacaccggg
    16021 cgcacgccgc tggccgccgc gcgcatgatg gcctcgccgg tgcgtgtctc gccggtaaag
    16081 gtgatgccgt tgacgccctg gtggcgcgtg aggaactcgc ccgccgagtc cgggccaaag
    16141 ccgtgcacca cgttgtacac acccttgggc atgccgacct ggttcatcac ctcgcccaag
    16201 agcgtggccg tggccggggt ttcctcgctg ggcttgacga ccacggtgtt gccgcaggcc
    16261 agggccgggc caaccttcca ggtcatcagc aagagcggca ggttccacgg gcagaccacg
    16321 gcgatcacgc cgcgcggcac gcgcacgccg tagctcaatg ccgtcttgcc gtcgggcgtg
    16381 cgcatctgga acgactcggt ggcggcgctc ttgatggtgt cgatgaagat ctggaagttg
    16441 gccgcaccgc gcggaatatc caggtgcgag gccagatggt gcggcttgcc ggtgtcggca
    16501 atctcagcct gcagaaagtc gtcaaagcgc cggttgatct ccagcgccac ggcatccagc
    16561 agcttgcagc gctcgaccac cgacagccgc ccccagtcgc ccttgagcgc ggcctgggcg
    16621 gcggccaccg ccgcattcac ctcggcctgg ccggcctcgt ggatggagcc gatgacgctg
    16681 ccatcgaccg ggttgatgtt ggcaaaggtc ttgccgctgg cgttcctgac gtactcgccg
    16741 ttgatgaagt tgagaaactc tttcatgtcg ctgccctgat tttttggaat ggttgaacgc
    16801 ctgccggccc cctgcaagcg ccggcccggc gggccggcgc aactggctta gaacgccttg
    16861 accagcgtga agcgcagcag cgagcccttg ggctgcgcgc ccgtgccggc cttggcgctg
    16921 acctccttgt agagcgcgcc cttgaggaac acgcccgcgc ccggtatcgg gtacaccagc
    16981 tcggcgccca cggcgtggcg cgcggccttg ttggcgctgg cgccggcgcc gctgtcgttg
    17041 gtggtctgcc actggctgta gcccaccagg ccggccgaga ccgcgccgaa tgacttgccc
    17101 agcccccact ccagcgtcag ctggtcgccg gggcgcatgc cggcgctgtt gcggccgttg
    17161 aattcgtagc gcatcagcgc cgcaccggag acggtcttgg caccgtcgaa gtaataggtc
    17221 aggccgcccg tgagcatggt gctcttgaag cccttgccgg gcgatgccgg ggcgctggtg
    17281 ctggcggtgt cgaaccacat gccggcggcg gccacggcgt cccactgcgg gccatgccag
    17341 cccagcacca gcgggcccag gtagacatcg cccacgccgc tgcggctgtc ggagatgccg
    17401 gcggcgttga tggtgagcga ggtgcgcatc accggcacga tggcctccac gccatagtcg
    17461 gcgcccagca gcttgtggtt ggtgacccag acgaagcggt tcgccagcgc cgtcacggtg
    17521 ccgcggttgt ggccgggcag gttgctgctg ctgccggggg cgcggaagtc gtctatctgg
    17581 taattgacca ggtagcccag gtagtacatg cccggtggcg gcacgctggc cgcctgcatg
    17641 ccctccacgc cgggcacgta atggccgtcg gccagggctt gcgtggcaaa aaggctgccc
    17701 agggcggcca gggccgcagc ggcgatgtgg gatttcttca tgttgtggtt tccttcagtt
    17761 atcgaggccc aacgccacca gggcggcgcg ggcgcatatt tcgtcctgct cggccgagcc
    17821 gccggagaca ccgatgccgc cgatcaacac cccgccctcg cgcatgggca ggccgccgcc
    17881 aaagaccacg ttgcgcggac gcaccggcat gcctaagcgc aacgcctcgt caccctgcaa
    17941 gatgccggcc cactggctgg tggcaaagcc aaagccggcc gccgtgtagg ccttgtcgac
    18001 ggcgatgtcc accgagtgca gaaaggcgcc gggcatgcgc aggaacgccg ccagcacgcc
    18061 cgaggcgtcg gtcacggccg cgctgatgcg tatgcccagc tcgccggcct tggccaccgc
    18121 cgccagcacg gcggcgttgg cagcctcggc ggtgatggcg cgttgctcca cgctgtgcgc
    18181 catgtcaggt caccacgctc aggaaggcgt cgttcagctt gcggtcgtga tagaagatgc
    18241 cgcgcccgac ttcctcccaa ctccaggtga tgggctccat gtccgggtag tggtcgtagc
    18301 cgccggcaaa ggtctccagg cggttgcccg aggggtcgaa gaaataaatg gtggtgccgt
    18361 tggtgatgcc gtggcgcgtc gggccgatgt cgatggagac acggttcttg ctcatgatgt
    18421 ccgcggcgcg cagcacctgc tcccagctgc cgagctggaa cgacacatgg tgcagcttgc
    18481 ccggctcgcc atggcgcacc atggcgatgt catgcgcctt ggcgctgcag gtcagccaca
    18541 cgcccaggtc ggtctgaccg tcttcgagct tcacgcgctc gaccagtgtg aagcccagca
    18601 ccttggtgaa caggtcggtg gcgccgtcga gatcggtgcc gtagatcagc gcgtggtcca
    18661 ggcggatggg ggtgatgccc ggctggtcga tgatgtccat gtcggggctg ctgtttccca
    18721 cggcgctgcc gacggcggtc ttgtcggcgt acagctcgat catgtggccg gtggggattt
    18781 gaaagcgcac acgctcgccg gtctccagca gctcaccagc ggggatgcgc tcggtcttga
    18841 cgccgtattc gttgagctgt ttttccaggc tggccagggt ggccttgtcg agcaccttgt
    18901 agccaaagaa gtccatgccg gcacggtcgg actggcgcag gatgatgctg ttgtggtcgc
    18961 gctcggcgcg ggtcttgaag taggcgcggc cctggctgtc gcggccctgg tacttcaggc
    19021 ccatgacgtc ggtgtaccac ttcactgatt cctcgatgtc cagcacacga atctgtgcgt
    19081 gcccggggcg caaaactcca gtcattgcca tggtctgtct ccttgtggtt gtcaggtggg
    19141 ttgggaaagg gaggcgtcgg tcgttgacgg cgaccggcac acggttttct tcatgctgcc
    19201 gaccacgctc aggcgcaggt cgctggtcgg cttgatgcga caggagagca cgcagccctc
    19261 ggcctcttcc tcggcgctga tgtgggcgcg gctcatcacg cgcttgtcgt agctgccctc
    19321 gagcacatgc accttgcaca cgccgcagcc gccctggcgg cagcccacgg ggatgccctt
    19381 cttgcccagc gcctccatgc cctggagcac cgagcgctcg tccaggcagc ggtagctctc
    19441 ctgggtttcg gcgatcgtga cggtgtagta ctggcccatg gtggcgtccg gtcagatctt
    19501 cttgaacagc gggctgcgca cctgctgcgc atcggccgcc gagatgaact tctcggtgta
    19561 gatgtcgcgc tcgaacaagc gcccctgcat cagcgtgctg atgcaggcgt cgatcatcag
    19621 cggcgggccg cacagatagg ccttgtggcc gcggaagtcg ttggcaaagt gcgccttggc
    19681 ggcgtcgtgc acgaagccgc gcgcacccgt ccagccgctg tcctcggcct cgcccgagag
    19741 cgccgccacg tagctgaaat tggggtgctt gtcggccagc tgctcgaact cggcgtggtg
    19801 gtagagctcg tcctggctgc gcgcgccgtt gaccagcgtg atgggcttgc tgcaacccgc
    19861 ggccagcaga tccagcacca tggagcgcgg gctcgacagc cccgatccgc cggccaggaa
    19921 caggtagccc aggccctcct tggcgtgcgc cgattcgcgc acgaagaagc gcccgtaggg
    19981 gcccgagagc tgcactgcat caccgctctt gagctgctgg tgcacccagg tcgtgccctt
    20041 gcctccgggc acatggcgca cgttgagctc gatctcgtcg cggccgcccg ctgccgtagg
    20101 ggcgttggcg atcgagaagg cgcgcggcgc ggcctcgccg gggatgtgca ggttgatgta
    20161 ttgcccggcc tggaactcca tgggctgatc cagcttgagc cacacgccct tgatggtggg
    20221 cgtcagatct tcgatgcgca ccaccgtggc gcggtagtcg tgcacgagca ggttgcgcgc
    20281 gtcgggttct tcgtcgatct cggcctctat aaccacgtcg gactgcagcg tggcacagca
    20341 ggccagggcc ttgccttctt cgcgctcata gtccatcagt gcaaagccgc tggcctcgcc
    20401 atgctcgatc tcgccgtccg tcacctggat cttgcaggtg gcgcacaggc cgtggcagca
    20461 ggcgtgcggc aggtagatgc cggcgcgcag cgcggcatcc agaatgctct ggccttcctc
    20521 gacctcgatg gtctggccca gcggttcaat ggtgagctga tggctcatga caatctcact
    20581 gattcaaaat agatagctac ttgcgcttta tggacaaggg attgacctga atttgattca
    20641 aacccttgcc aaaagacgca tctcagaagc aggagccgct caggcccgtc aggcccggcg
    20701 tgcgaaagcg gatcacgctc ttgtggccca ggccatgctc ggccagggtc ttgtccatgt
    20761 cgggcgtgaa gggcttgccg gacatgaacc actcggcctg gtcccagtcg atcctggcaa
    20821 agtccgggtg ggcgccatac atgccgggca acgcgccgtt gacgaggtcg ccaaacttca
    20881 tcgtcggcgg catgggggcg cagaacggcg cgcagaacat cttgtgatcc tcccagccga
    20941 tgtagagcag cggcgcgggg aatttgtcca ccgtgtcttt ttgctcgaac tcgtaggggg
    21001 cgatggattt gacggccatg gggtatctcc ttgtctcttg cctggtgcgc gctcagttgc
    21061 cggtggcctg ggagcgccag ttctcgaagt tcttcttgtc cggcgagtcg gcgtagtcca
    21121 tgttgtcctc gccctggcgg atcttgtacc agcgcagcac ctcggccagc gggttgaagt
    21181 cgggctgggt cggatccacg tcctcgggga agcagttgcc ctggtagatc tggtgcacgg
    21241 gcagccagct ctgggcgtac ttctcgggct cgttgtcgaa gatgtgcttg cagccgtcgg
    21301 aacagcagtg gtacttcatg cccttgtatt cgctctcgcg gtaggccacc ttggtcgggt
    21361 cgtcgggctc ggtgaagaac atcggcacct ggcagacctg gcacagcatg ggcagggtgc
    21421 cgttgtagaa gcgcttgccc tcggcctgct ccttggcgta gtgctgccac agcgggcggt
    21481 agtacttgtc aaaggtttcg gggtacttgg ccgagagcca gtccatgtcc ttctcgctgg
    21541 gcgcccaggt gtggaagggg gcggcggcgc cgtagttgta gaacacgttc caggcctgat
    21601 ggctgaggtg atccttgtcc ttgcaggcct gttcccagcc cttgggcacc ttgatgccgt
    21661 agcgcgccag gtcgttgaac agggcggcgc cgttttgctc ggcgtagatc tcccaggcct
    21721 ccttccagct catcacgcgc ttgggcagca tgtagtccat catcatggcc accagggtca
    21781 gcacgcggta gccgcgccag aaccatttgt ccagccaggc ctgcacgatg ggcaggttgt
    21841 ccggatcctg ctccaggatg aacttgatga tctccagccc cagcgtcatg tggcgcgact
    21901 catcgctctg cgccgagaag ccgaaggcca tcgcccccat atcgccgttg taggccgcgc
    21961 ccgagatgaa gggcacgaac agcaggttgg tcagcacata ttcaaaggcg aagccgatgg
    22021 cgacgatgaa ctcgaacggc ccggcgctga cggcgtcgtc aaagaacgac ttgggcacgc
    22081 tcaggtacca gacgcggtca tgctggtggc gccagtcggc aaaaccgttg tagtgcttgt
    22141 tgtaattgga caggctgtgg atctgcgtct gtgcgtggcg gatctcgtcc aggctctgca
    22201 tcatgcaggc cacacgcggg ccggcgccgg ggtactggcg cccgacatgg gcaaagccgc
    22261 ggtgcgccat gtattccagc ggcgagacgc cggtcaggaa cagcttcaag gtgttgatgt
    22321 agcgcccatc ggtcacgccc aggtggccgt tgttctgcgt gaacgcatcg atgatggcgt
    22381 agagcttgcg ctccttctcg gcctggtatt tccagtacga gtccatcgtc atgcggaacg
    22441 ggtcttcgaa cttgtcccag tcgtggatct tgatgccctc gtactccatg tacgggaaca
    22501 cgtccttgac ggcctggtag ctgggattcc aggccaggtc gcgcgtcatc aggttgtagc
    22561 gctccttgag gcccagcttc ttggtggctt tcatatccat ggtgtgtgtc tccttgcgtc
    22621 gtccgtgggg ggtggcggtg gcgcgggttt attgatgcca ggtgagggtg aattcgtcgt
    22681 cggtttcgtc cacgttgccg ctcatggtga tgaggtgcac ctgcatttcc tgcaggtcga
    22741 acgcgcgccc gatctcctct tcgatggtgg acttgcgcac caccagatgg ccaggtacgt
    22801 cgatcttcac catggccggc tggtcgctga cgatggcgcc ggggttgtcg gccaggatgg
    22861 cgttgacgat ggcgcgcgag tcttcgttct tctgaaatgc aatgaatacg ttggacatgc
    22921 ctgtgctcct gtactggtgt cggggtcggg ctcaggccag ggtcaggccg gccttgttca
    22981 tgcgcccatc cacggccgcg accgagcgcg ccagcgcatc ggcaccatcg gcgcccagcg
    23041 ccagctcggc gatgggggcc agggcctgct gcacgcggcc gcgccagtgg gcgtaccagt
    23101 ggctgagcag ctccttgttc tcggggctct cggcggcggc gaccttcatc accgcgtcca
    23161 cccatttgtt ggcgtcctga ccccactcgg cctggaagcg cgtgagcatg gagaccaccg
    23221 ggccggcctg ctcgctcagc acctgatcga cctccttgta ggccagggcg aacagcacgc
    23281 cgtcgagcac caggttctgc gccacgaaca gctcgaacca gtccttgagc acccaggtgt
    23341 cttccaccag gcggcgcagg ccctgccagg cgggcgcctg cagccaggcc tgcttgccgg
    23401 cctcgagctc gccctggttg cccagcagca ggcccaggcg ggtgaggtac tgggcaatgc
    23461 ccagctgatc catgccggca tacaggcagg gctgggtgat ggcggtgccg tagccgtagg
    23521 cgcactggta ggcgccgttc atgttggcgc cccaggcgac atggcgcagc ggcacataga
    23581 agtccagcgc cttgcgccgg gcggcgtcgt catagcgatc ggccaggccg cgctcctcga
    23641 cgaattcaaa gtccgcctcg gccacttcct gcatgcgcgc acgcgcctgg gtgtaggcgc
    23701 cgtagtagaa ctggcgcggg tccttcagcg cataccagtc ccgcatcacc aggcgggtgc
    23761 ggcgcgggtc gaacagctcg tgctccggat cccaggtggg gcggtagtgg aagttggcat
    23821 cgggctgcac attcatcgtg ccctcgatgt agcgcgaggc cggcttgtcg gcccccagcc
    23881 gctcggcgat atggctgtag gtctggcgca gcggggtgat gctgacggtg cgcaggtcga
    23941 tttgcatggg gcttgtctcc ttgggttttc ggtaggggct gacggtcacg gctcctggcg
    24001 gaaatgccgc tcacgggcat cgcgcaggct ccagtcccat tcgtgctcgg cgctgtcctc
    24061 gccatgctcg ggcaggcggc catgcgtggg caccacggcc tgcatggcgc agaactcctc
    24121 gaactgctgg cgcggcatca ccatctcgac gtagagcccg ggctcgccca cggcgaactc
    24181 gaactccacc atgccgttgt cgtgctcctg cacgatgcgg atgaagcggc gctggacatc
    24241 ccagcggtct tcggaagggg gcggcgtagt gctcatgcca ggagctatgc agaacatgtg
    24301 ccagctcgtc ccgcaaaggc tgaaatctcg ataattcagg ggtaaacccc taaaaattca
    24361 ccaaaagaac agggatttcc aagccccatg aatcacaaaa tccgcctgta tccaggcgta
    24421 aattcacgat ttgcgtgatt catcgaggag gtgattcacc atttgatgaa attcacccaa
    24481 tttctccgct tgcactgaaa aatggtttat cgataattca acctcatgcc cacactcccc
    24541 gccctgccct cggatgccga cctgcggcgc cagatccagt tctccgcggc cgacggccgc
    24601 atctggctgg cgggccagcg catggtgttg atgcacgccg cctcgctggg catattgcgc
    24661 cgcgagctga tgcacaccct ggggcccacg gccgcgcgcc gcctgttgct gcgcgtgggc
    24721 tacgcctcgg gcgagcgcga cgcggcgctg gcgcgccagc tgcgccccgg cgccgacgcc
    24781 ttcgccatgt tcagcgtcgg gccccagctg cacatgctgc agggcgcggt gcaggtggcg
    24841 ccccggcgcc tggagctgga catggccagc ggccatttcc acggcatctt cgactggcag
    24901 cattcctggg aggtggagac ccatgtgcgc gacttcggcg cgcagcacaa cgccgtgtgc
    24961 tggacgctgc tgggctacgc ctcgggctat accagcgcct tcatgggccg caccgtgctc
    25021 tacaaggagg tgcaatgcgc cgcctgcggc catgcccatt gccgcatcga gggccggccg
    25081 gtgcaggact ggcccgatgg cgcccaggtg gccagcgact acgccccaga cgcgctggcc
    25141 ctgccggagg gcgcgcgcac gccggtcgcc tggccgccgc ccgtgccggc cgaggaccgg
    25201 cccgagccgc cggccgacgc cctgggccag ctcatcggcc gctccaccgg cttcaacgcc
    25261 gccacacagc tgctgcgcaa ggccgccagc acccaggtga cggtgctgct caccggcgaa
    25321 accggcgtcg gcaaggagcg ctttgcgcgc gccctgcacg ccctgagccc acgcgcggcc
    25381 aagcccttcg tggcggtgaa ctgcgcggcg ctgccgggca gcctggtcga gtcggaactg
    25441 tttggcaccg agaaaggcgc ctacaccggc gccgacgccg cgcgcgccgg gcgcttcgag
    25501 cgcgcccacg gcggcacgct gttcctggac gagctgggcg agctgccgct gccagcccag
    25561 gccaagctct tgcgcgtgct gcaggacggc caggtggagc gcctgggcgc gacccagccg
    25621 cgccaggtgg atgtgcgtct ggtggcggcc acgcaccagg atctggccgt ggcggtgcgc
    25681 gccgggcgct ttcgccagga tctgtactac cgcctgaacg tctaccccat acgcatcccg
    25741 ccgctgcgcg agcgactcga tgacatcgag ccctttgccc gccacctgct ggaacgcttt
    25801 gccgcgcgcc acgacaagca catcgccggc ctgagcgacc gcgccctgca cgcgctgcgc
    25861 cagtacgcct ggcccggcaa catgcgcgag atggagaacc tgatcgagcg cggcgtgatc
    25921 ctggccgacc cagggcagcc ggtggacgcg gcccacctgt tcccggacgg caccggcgcc
    25981 gccagcggca cgccgacact cacgctgcag gcctcgggcc gcctgggcga gacccgcgcc
    26041 agcggctgcg acgacacgct gatcgcgcgc ctgctgggtg acggcctgga tctgcaggca
    26101 ctggagaccc agctgatgga agccgccgtg cagcgcagcc acggcaacct ggctgccgcc
    26161 gcgcgcctgc tgggcctgac gcgtccacag ctgagctacc gcctgaacaa accacgcgaa
    26221 tcctgaaccg gagcccccgc catggccaaa cgcagcgccc tgggcgccat cgacgacccg
    26281 gcaccgtccg ggcgcaccct gatagagcag gcctacgcaa ggctgcgcga cgacatcgtg
    26341 accggccagc tcggcccggg cgaacgcctg cgcgtggagc acctgaagac cgactatggc
    26401 gtgagcgccg gcacactgcg cgaggccatc acgcgcctgg ccagcgacgc gctggtggtc
    26461 accgaggggc agcgcggctt tcgcgtcgcg cccattgctc tggaagacct ggaggacatc
    26521 accaacctgc gcgtgcagat cgagaccatg gccctgcgcc agtcgatacg cgcgggcgac
    26581 gggctatggc gccagcgggt ggagcaggcc tttgccgcga tctcggccga ggagcccatc
    26641 gccccggagc gccggcgcca ctgggaaaac ctgaacgtgg actttcacga ggccctgctc
    26701 tcgggctacg cctcggcctg gacgctgcgc gtgctgcgcc tgctgtcgcg ccacaccgag
    26761 cgctaccgca gctttgccat ggccctgccc ggcgccgtgc gcgacgtgca tgccgagcac
    26821 acggcaatcg ttgaatttgc cctgtccggg caggacgcgc gcgccgcgct ggcgctggag
    26881 gcgcatatcc gcaccacgcc cgacctgctc atgcaggcgc tgcgcgaggg ccgggcatcg
    26941 ctgcccggca atggcgcacc accaccgccg ccgtagaaat ctgccgaccc atcaccgggc
    27001 gaataagccg cccggcctgg ccatcaacgg cgcgactgca gatacagccc ctgcacgcgc
    27061 ggcacgttct gctccagctc gcggatgcgg tctggccccg tggggtgggt ggaca
//
LOCUS       DAOMTQ010000022        26786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_177_length_26786_cov_25.427893, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000022 DAOMTQ010000000
VERSION     DAOMTQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 26786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26786
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_26786_cov_25.427893"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PK518_03875"
     CDS             <1..1139
                     /locus_tag="PK518_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HRM48080.1"
                     /translation="IMRGCFGGCTFCSITEHEGRIIQSRSEDSILREIEDVRDKVQGF
                     TGTISDLGGPTANMYRLGCRTPEIEAACRKPSCVYPGICQNLTTDHDPLISIYKKGRG
                     IKGIKKILIGSGLRYDLAIQSPEYVKELVQHHVGGYLKIAPEHTEAGPLTKMMKPGIG
                     NYDKFKVLFEKYTAEAGKKQFLIPYFIAAHPGTSDEDMMNLAVWLKKNGFRADQVQTF
                     YPSTMATATAMYHTGINTLKGVHRDERGEKVDVVRGEKRRRLHKAFLRYHDPANWPLL
                     RDALKAMGRADLIGNGKHHLIPTYQPMGKQCASKDKGKYLVIPTMVLDQARADAAKKP
                     QAGGQQGTGGRAAAKPAAKGRATPQPRPGQILSQHTGLPPRMQR"
     gene            complement(1191..1853)
                     /locus_tag="PK518_03880"
     CDS             complement(1191..1853)
                     /locus_tag="PK518_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock and DUF1294 domain-containing
                     protein"
                     /protein_id="HRM48081.1"
                     /translation="MHTQGIIRRWDSAKGFGFIQGAHSSNVFFHVRDYRGATPPAEGM
                     RVEFEEIQVGGKGPRAMAVRPLGARAAAPAPARAGRPAPAQPATRRRTAPQARDNTPA
                     GASLAMGLMLAWLALLAWGSWAQRFPLWLLGAMAALNLVTYASYKLDKSAAQSGGWRT
                     SEKQLHLLALLGGWPAAWWAQQWLRHKSSKQEFRVVYWATVLLHCAGLGVLVVRPDLL
                     PI"
     gene            complement(1908..3104)
                     /locus_tag="PK518_03885"
     CDS             complement(1908..3104)
                     /locus_tag="PK518_03885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HRM48082.1"
                     /translation="MSLFTAVEMAPRDPILGLNEQFNADTNPAKVNLGVGVYFDDNGK
                     LPLLACVQAAEKAMMDKPAARGYLPIDGIAAYDAAVKGLVFGADSEPVKTGRVATVQA
                     IGGTGGLKIGADFLKKISPAAKVLISDPSWENHRALFTNAGFTVESYAYYDAAKRGVN
                     FDGMLASLNAAESGTIVVLHACCHNPTGYDITPAQWDQVIAVVKARGLTPFLDMAYQG
                     FGNGIAEDGAVIQKFVAAGLAFFVSTSFSKSFSLYGERVGALSVLCQSKEEADRVLSQ
                     LKIAIRTNYSNPPIHGGAVVAAVLNTPELRALWEKELGEMRVRIKAMRSKLVDGLKAA
                     GVKQDMSFMTEQIGMFSYSGLSKEQMLRLRSEFGVYGTDTGRICVAALNSKNIDYVCQ
                     AIAKVI"
     gene            complement(3232..4419)
                     /locus_tag="PK518_03890"
     CDS             complement(3232..4419)
                     /locus_tag="PK518_03890"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRM48083.1"
                     /translation="MASDVILVLNCGSSSIKFALFDAATIPMPRQPRWSGKVQGIGGP
                     TPTFDEAGQPPQPITLDTEHPNTAALALIRERVLKRLQGQRPCAVAHRVVHGGQHYFA
                     PTLVTQQVIDELRGYIPLAPLHQPFPLKAMSLLMQQHPDVPQVAVFDTAFHRTAEPVE
                     QMFALPWSAWERGVRRYGFHGLSYDYMGHALPERHGDAARGRTIVAHLGSGASLCAMQ
                     GLKSVATTMGFSALDGLMMGTRCGALDPGAVIYLMQIEKLSLDEVARMLYQDSGLLGV
                     SGVSPDPRVLLPLEHENERVRTALALYVRRIVREIGALAAVLGGLDMLVFTAGIGENN
                     AELRRRVCVQLGWLGLNLDQDANASHAAVISTSASRVLVGVEPTNEEWIAASQATRLL
                     AQG"
     gene            complement(4446..5405)
                     /locus_tag="PK518_03895"
     CDS             complement(4446..5405)
                     /locus_tag="PK518_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HRM48084.1"
                     /translation="MITSTNPAPANGQQLRGIDRLIAHVRNLEPVRMAVVHPCDALSL
                     GGAMDARDAGIIIPVLVGPQARLQAVAAEGGISLAGCEIVDAPHSHAAAAAAACMAGK
                     GEVQALMKGSLHSDEYLGAILAPNVGLRTERRLTHCFIMETASYPRPFIITDAAINIA
                     PTLDDKADIVRNAIDLAHVIGVAQPRVAILAAVETVNARMPATLDAAALCKMADRGQI
                     TGGLLDGPLAFDNAVSPEAARVKGIQSEVAGHADVLVVPDLESGNMLAKQLEYLGGAD
                     SAGIVMGARVPVVLTSRADSRQTRLASCAIALMLAQRYRVTPP"
     gene            complement(5402..7603)
                     /locus_tag="PK518_03900"
     CDS             complement(5402..7603)
                     /locus_tag="PK518_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HRM48085.1"
                     /translation="MQRAVLYADIMRARGNQYRAHMQETAPNVLNFAAELVMRGDTLP
                     RPVNYGLVRIQPQPGVASDETRRPFVVVDPRAGHGPGIGGFKPDSEIGAALAAGHPCY
                     YVGFTPNPIPGQTVEDVMRAEAAFLRKVIELHPDAHGKPAVIGNCQAGWQIMMTAAVW
                     PELFGPIILAGTPLSYWAGWRGKNPMRYGGGLTGGSWLTALASDIGAGKFDGAWLVQN
                     FENLNPANTLWEKKHHVYANVDTEGPRFLAFEKYWGGHVCLNDVEMQTIVDDLFIGNK
                     LSSAELVTSDGVRIDLRNITSPIVVFCSYGDNITPPPQALGWITDLYRSDDELLGHDQ
                     TIVYATHQSIGHLGIFVSGAVGRKEHAKFTSNIDLIDMLPAGLFELSVQDKEPDMAHA
                     EIVPGDYLMTVHQRTLDDVRAIVSPDPVQVAASDRRFAAAAHVSQVNLALYRRYLQPW
                     VRGAVTPQLADALRAWHPLRIGYEMWSDRNLLAPVVEQQAESVRADRKPVGADNPWWS
                     WQEACSDAITHLLQVFSQSRDEAEERAFEAIYSHPLVQALAGQADPAAALRPHPGDTP
                     EHRAWMAQRLGGLRVSIEQGGLPEATVRALIFVLQPRREADERQYRRALLLRQTELAS
                     RLDIEGARNLVRRQALLMHIAGPEAIAALPTLLARAPAERIQAAALHLGALLQAGNAL
                     EGEEAARWHTMRQLFEQAAQAGQPALPTPETPPAVQPPKRAASKRKGRQST"
     gene            complement(7922..9697)
                     /locus_tag="PK518_03905"
     CDS             complement(7922..9697)
                     /locus_tag="PK518_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM48086.1"
                     /translation="MNDLVTSQPQAPEPRAQAPGDFLDMIDNLAYAARARLTAGAAPT
                     AGALAWFDWSMHLALSPGKQRSLWLDAWRKQWQFARYAQQSGLQASCPACVEPLEHDR
                     RFADPAWQQWPFNLIQQGFLLQQQWWKEATTDVRGVSEHHEKMVEFAARQWLDMMSPA
                     NAIATNPEVLRATASSGGLNLWRGAMNFLDDAQRQLTGKGPAGAEGFEVGKDVAVTPG
                     KVVLRNRLIELIQYAPTTRKVGAEPVLIVPSWVMKYYILDLSPHNSMVSYLVAQGHTV
                     FIISWKNPDADDRDLGLDDYRQLGVMAALDAVGTIVPKHRVQAVGYCLGGTLLATAAA
                     AMARDGDERLASLTLLASEIDYRESGEVKLFVDDSQLAWLEAGMWRKGYLEGWQMAAS
                     FQMLNSRDLIWSRRVREYLLGERGSLVDLMAWNADTTRMPYRMHSEYLRHLYMNNDLA
                     EGRWQVDGQPVTVADIRVPLFVVGTLRDHVAPWRSVYKIHLLNDSDISFVLTSGGHNA
                     GIVSEPGRPRRSYQIATRKAGARYRNPAQWQAETPVHEGSWWPAWSAWLAHNASAQVA
                     PPPMGGKSGSGKTLGAAPGTYVMQK"
     gene            complement(9762..10790)
                     /gene="adhP"
                     /locus_tag="PK518_03910"
     CDS             complement(9762..10790)
                     /gene="adhP"
                     /locus_tag="PK518_03910"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HRM48087.1"
                     /translation="MNKTMKAAVVRAFDQPLAIEEVPVPRPGAGDVLVKIEACGVCHT
                     DLHAAEGDWPVKPNPPFIPGHEGVGFVAAVGAGVKHVKEGDRVGIPWLYSACGHCTHC
                     LGGWETLCESQQNTGYSVNGGFAEYALGNADYVGHLPANVGFVEIAPVLCAGVTVYKG
                     LKVTDTKPGDWVVISGIGGLGHMAVQYARAMGLNVAAVDVDDTKLRLAERLGATVTVN
                     ALNTDPAAFLKKEIGGAHGALVTAVSPKAFAQATGMLRRGGTMSLVGLPPGDFPLDIF
                     GMVLNGITVRGSIVGSRLDLQESLAFAEQAKVAATVASDRLENINDIFARMHAGQIEG
                     RIVLDMNA"
     gene            complement(10848..11993)
                     /locus_tag="PK518_03915"
     CDS             complement(10848..11993)
                     /locus_tag="PK518_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM48088.1"
                     /translation="MNRARSSIRHLVNIYRLGVKELWSLWRDPMMLVLIVWVFTGSVY
                     TAATAMPETLHNVPIAIIDEDDSALSQRIVSAFYPPQFIAPRLVSMTEADRGMDAGLY
                     TFSLHLPQGLQRDVLAGRAPQLQLNVDATRMSQAFTGSGNVQQIVLAEVGEFVQRQRV
                     GTAAPVELAVRARFNPALEKSWFGSLNQIINQITLLSIILTGAALLREREHGTIEHLL
                     VMPVTPTEIMLSKIWAMGLVVLAAAALSLNLVVRAMLQVPVQGSLPLFFTGAALCLFA
                     TTSMGIFLATLARNMAQFGMLLVLTIMPLQMLSGGTTPRESMPELVQTIMLAAPTTHF
                     VEIGQAILYRGAGLDVVWQPFLWMALIGAVLFALSLARFRKTIAQMA"
     gene            complement(11995..14745)
                     /gene="rbbA"
                     /locus_tag="PK518_03920"
     CDS             complement(11995..14745)
                     /gene="rbbA"
                     /locus_tag="PK518_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HRM48089.1"
                     /translation="MVTSAQLEPVIRLSGVGLHYGKRCALAGIDQALPAGCMVGLIGP
                     DGVGKSSLLSLIAGARKIQDGTVWVLGGDMRERSHRNRVCPRIAYMPQGLGKNLYPTL
                     SVEENLQFFARLFGHDAPERRRRIDDLTRSTGLHRFLDRPAGKLSGGMKQKLGLCCAL
                     IHDPDLLILDEPTTGVDPLARSQFWDLIDRIRSQRPQMSVLVATAYMDEAERFDWLMA
                     MDDGRVLATGTPQELFARTATDSLEAAFIALLPPEKKHGHAPVTIPPLEADAGDIAIE
                     AKNLTMRFGDFVAVDQVSFRIRRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGQAWLF
                     GHEVDPKDIDTRRRVGYMSQAFSLYGELTVRQNLVLHAQLFNVPEAHIAQRVQEMAER
                     FDLTGAMDSLPASLPLGMRQRLSLAVAMVHQPELLILDEPTSGVDPVARDAFWRLLIE
                     LSRRDQVTIFISTHFMNEAERCDRMSMMHAGKVLDSDAPAKLVQKRGAKTLEEAFIGY
                     LVEAEGGAQEAQDAPPVSAPAASTQEVATAPARSTPFSLQRLLSYLWREALELRRDPI
                     RLTLALGGSILLMFVIGFGISMDVQDLRYAVLDRDNTTLSRDYALQLSGSRYFVERPA
                     LTDYDELDRRMQSGELSLAIEIPQGFMRDALRGHGVQIGAWVDGAMPTRAETVRGYVQ
                     GMHQSWLATQAAAMGVRTAGTAQVETRFRYNPDVKSLPAMVPAVIPMLLLMLPAMLTA
                     LAVVREKETGSITNLYVTPVTRSEFLLGKQLPYVGLAMVNFLLMALLAVTVFGVPVKG
                     SFVTLTLAALVFGFVSTGMGLLASTVTKSQIAAMFLAMIGTIIPATQFSGLIDPVSSL
                     EGSGKLIGQVYPATHMITISRGVFSKALDFADLSGAFWPMLLSVPVIIGTAIVLLKKQ
                     ER"
     gene            complement(14750..15838)
                     /locus_tag="PK518_03925"
     CDS             complement(14750..15838)
                     /locus_tag="PK518_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM48090.1"
                     /translation="MNATLKKWLVPAAIALLAGGAYMAWNATRDQGPGEGFVHGNGRI
                     EATEIDVATKLPGRLAEVLAAEGAFVKAGDVLARMDMASLQAQRSEAVARHAQAQHGV
                     ATAHAQVALRESDLRAMQAQVLLRESELDAAQRRLARSTDLSRDGASSQQELDDDRTR
                     MRGAQAAVTAAKAQVDAARAATEAARTQVTGAKAQVDAAQASIERIDADMKDAVLTAP
                     RDGRVQVRVAQPGEVLGAGGRVLNLLDLSDVYMTFFVPETVAGRIALGSEVRIVLDAM
                     PGTVIPAQVSFVASQAQFTPKTVETASERQKLMFRVRAQIAPQLLQRYIEQVKTGVPG
                     VAWLKLDSNAQWPASLALDPRTTAVGGS"
     gene            complement(15843..17348)
                     /locus_tag="PK518_03930"
     CDS             complement(15843..17348)
                     /locus_tag="PK518_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRM48091.1"
                     /translation="MNDFPFSIARRRPAAAGRGAWAQRLPAGALVAGALWALAGCTTL
                     PPGYQPPAAQIPAQFSSASPADASQVGELAWRDYFTEPELQALITKALANNRDLRAAM
                     LRVEQARAAYGISRADRVPAVGLGLDSQRTRMPGDLNFTREPIISSQHQLGVGLTSWE
                     IDLWGRVRNMNEAALQTYLASADGQRATTLMLVAQVADGWLSVRELDHRIQLADQALA
                     TRTEARRRFARRVEVGSSSKLDFVQVELLWQQASALVTQLQLQRSTQLHALGLLVGEP
                     GLSLAPATQPLSALAPLPPVAPGLPSQLLENRPDIVAAERQLQAANARVGAARAMFFP
                     RIALTTMAGTASADLDGLFASGSRAWTFAPSVSLPIFDGGRRQANLELAQVQREQALN
                     EYQQAIESAFRDVADALAAGQSLQEQLQTLRATEDTLTERARLAGRRYEAGAARYFEV
                     LDAERDLLATQQQVVQAEHALLTARLRLYAALGGGSRTLAPLPLPLQKSEE"
     gene            17441..18061
                     /locus_tag="PK518_03935"
     CDS             17441..18061
                     /locus_tag="PK518_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM48092.1"
                     /translation="MSDSEINLPKRRLRLAPEARVPLILDAALQEFSRRGFSAARMDD
                     IAQRCGLSKGGLYAHFDSKDAIFKALLDRTLHRTDWARMPQLAAGAGTRAVAEWIVDH
                     LRAALLAPDVITLLRLLIPERERVPLRVDEWKNLTQLNTQHVTAVISENLKASGRQDS
                     VLARHPWLALSPMVHVLLWHAVFGEGSDPDPDFHQAHVDMLCELLA"
     gene            18141..20228
                     /gene="uvrB"
                     /locus_tag="PK518_03940"
     CDS             18141..20228
                     /gene="uvrB"
                     /locus_tag="PK518_03940"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRM48093.1"
                     /translation="MHEVTAEPAAVDGQFVQFPGSPFQLFQPYPPAGDQPEAIARLVE
                     GVQDGEVFQTLLGVTGSGKTFTMANVIARMGRPAIVFAPNKTLAAQLYSEFREFFPKN
                     AVEYFVSYYDYYQPEAYVPQRDLFIEKDSAINEHIEQMRLSCTKSLLERRDVVIVATV
                     SAIYGIGNPESYHRMIMTLRAGDRLSQRDAIGQLIRMQYQRNEQDFSRGKFRVRGDTI
                     DVFPAEHSELAVRIELFDDEVESLQLFDPLTGRVQQKIPRFTVYPSSHYVTPRDVVLG
                     AVETIKEELRERLEFFAKEGKLVEAQRLEQRTRFDLEMLSEVGHCKGIENYSRHLSGA
                     QPGEPPSTLTDYLPPDALMFLDESHQMIGQLNAMYNGDRSRKTTLVEYGFRLPSALDN
                     RPLKFEEFEGRMRQVIFVSATPADYEKQHSGQVVDQVVRPTGLVDPEVEVRPATHQVD
                     DVLQEIRLCVQQRQRVLITTLTKRMAEQLTDYLSDNGVKVRYLHSDVDTVERVEIIRD
                     LRLGAFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAARNLHGR
                     AILYADRVTDSMKKAIGETERRRARQLAYNQAHGITPRGIVKQVRDLIDGVYSDKAGQ
                     EALRLEQEAQRHAELDEMSEKDIAREIKRLEKRMLEHARNLEFEQAARVRDELAHLKD
                     RVFGAHGGDQVPA"
     gene            20470..21006
                     /gene="iscR"
                     /locus_tag="PK518_03945"
     CDS             20470..21006
                     /gene="iscR"
                     /locus_tag="PK518_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HRM48094.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQNNGPVTLAAISQRQQISLSYLEQ
                     LFGKLRRHELVESTRGPGGGYTLARKAGDITVADIIVSVDEPIDATQCGGKENCLGEA
                     GRCMTHDLWATLNQRMVEFLDSVTLQKLVDDQLAKGIQIEDRPVTRRAISTTPVVKPI
                     RVNAPNSVFALGNAFAKS"
     gene            21115..22335
                     /locus_tag="PK518_03950"
     CDS             21115..22335
                     /locus_tag="PK518_03950"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRM48095.1"
                     /translation="MDMTPHFPIYLDYGATTPVDPRVVDAMVPWLREHFGNAASRSHA
                     WGWEAEEAIEKARGQVADLIGADPREIVWTSGATESINLALKGAAQFYKTKGKHLITL
                     KTEHKAVLDTMRELERQGFEVTYLDVKEDGLLDLDALKAAIRPDTILISVLFVNNEIG
                     VIQDIPAIGALCRERGILLHVDAAQATGRVEIDMASLPVDLMSMTAHKTYGPKGVGAL
                     YVRRKPRVRLEAQIHGGGHERGMRSGTLPTHQIVGMGEAFRIIKQEMHEVNAKARVLQ
                     QRLLDGLKDIEQVFINGSLEQRVPQNLNMSFNYVEGESLIMGIKGLAVSSGSACTSAS
                     LEPSYVLRALGRSDELAHSSLRMTIGRFTTEEEIDYAISTIRHNVAKLRELSPLWEMY
                     QDGVDLSTIQWAAH"
     gene            22373..22774
                     /gene="iscU"
                     /locus_tag="PK518_03955"
     CDS             22373..22774
                     /gene="iscU"
                     /locus_tag="PK518_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRM48096.1"
                     /translation="MAYSDKVIDHYENPRNVGSFDKGDDSVGTGMVGAPACGDVMKLQ
                     IKVNAETGVIEDARFKTYGCGSAIASSSLVTEWVKGKTLDEAAQLKNSQIAEELALPP
                     VKIHCSILAEDAIKAAVKDYKAKHDAVVEAA"
     gene            22782..23105
                     /gene="iscA"
                     /locus_tag="PK518_03960"
     CDS             22782..23105
                     /gene="iscA"
                     /locus_tag="PK518_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HRM48097.1"
                     /translation="MAITLTEAAARHVIRYLARRGKGFGVRLGVKTTGCSGLAYKLEY
                     VDEQAPEDIVFEQHGVKVLIDPKSLAYIDGTELDFVREGLNEGFKFVNPNERDRCGCG
                     ESFRV"
     gene            23172..23690
                     /gene="hscB"
                     /locus_tag="PK518_03965"
     CDS             23172..23690
                     /gene="hscB"
                     /locus_tag="PK518_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRM48098.1"
                     /translation="MNLQSDDFELFGLQPRFAQDRAMLDARWKDLQREAHPDRFAAQG
                     PAAQRVAMQWSVRINEAYQRLRDPLKRAAYLCELHGAPIRAEDNTAMPAAFLMQQMQW
                     REALDEATGAAELDALEDETQAARGAALARCGQLIDVRQDYAGAAREVRSLMFIARFA
                     RDIDLRREQLGQ"
     gene            23759..25627
                     /gene="hscA"
                     /locus_tag="PK518_03970"
     CDS             23759..25627
                     /gene="hscA"
                     /locus_tag="PK518_03970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRM48099.1"
                     /translation="MALLQISEPGQSPDPHQRRIAVGIDLGTTHSLVAAVKSGVAECL
                     PDAQGRVLLPSVVRYLQDGGRHVGHEAAAAQTQDPVNTVASAKRFMGRGLADIAGREK
                     LPYDFVAAQAAGGMLSLSTVAGVKSPVEVSAEILATLRYRAEDSFDSDIYGAVITVPA
                     YFDDAQRQATKDAARLAGLNLLRLINEPTAAAIAYGLDNAAEGVYAVYDLGGGTFDIS
                     ILRLTQGVFEVIATGGDSALGGDDYDAALADWVLQQLGLQAHTPADKAAVRLAARACK
                     QGLTTTETVAFSAYLAGVEAKFDVKSSDFAAVTAALTQRSLAAVRRALRDAQITRDEV
                     RGVVLVGGSTRMPQVREAVADFFGREPLTNLNPDEVVALGAAIQANQLAGNSTSGDLL
                     LLDVIPLSLGLETMGGLVERIVARNETIPTAKAQDFTTYKDGQTALAIHVVQGERDLV
                     QDCRSLARFELRGIPPMAAGAARIRVTFTVDADGLLSVSAREQGSGVEASIDVKPSYG
                     LSDEEIARMLQDGFATAEQDMQARALVEARVDADRLLIATQSALDADGDVLATDERTA
                     IDALMRALRAVLDAGEGAASVEAATAALAKGTEAFAAQRMNRGIRQALSGKNVSAL"
     gene            25643..25981
                     /gene="fdx"
                     /locus_tag="PK518_03975"
     CDS             25643..25981
                     /gene="fdx"
                     /locus_tag="PK518_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HRM48100.1"
                     /translation="MPVIKILPHHEYCPEGAEISASAGTSICEALLEHDIKIEHACDM
                     SCACTTCHVIVRQGYETLNEAEEEEEDLLDRAWGLEPQSRLSCQAILAQQDVTIEIPK
                     YTINHARENH"
     gene            25981..26709
                     /gene="dnaQ"
                     /locus_tag="PK518_03980"
     CDS             25981..26709
                     /gene="dnaQ"
                     /locus_tag="PK518_03980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRM48101.1"
                     /translation="MTRQIVLDTETTGLSAQTGDRIIELGCVELVNRKLTGNNLHLYL
                     NPERDSHEDALKVHGISNEFLRDKPKFADCVQDILQYLQGAELIIHNAGFDVGFLDKE
                     LELVGLPPLATHVDSITDTLAMAKELFPGKRNSLDALCDRLEVDNSGRTLHGALLDAE
                     LLADVYINMTRGQDALLIMDEAPAGDDGIRVAAMDLSRIALPVLPANEQELAEHEDLL
                     TQIDKSSGGKTIWRTFAGGEKAMP"
BASE COUNT         4599 a         8754 c         9020 g         4413 t
ORIGIN      
        1 acatcatgcg cggctgcttt ggcggctgca ccttctgctc catcaccgag cacgaggggc
       61 gcatcatcca gagccgctcc gaggactcca tcctgcgcga gatcgaggac gtgcgcgaca
      121 aggtgcaggg ctttaccggc accatctccg acttaggggg ccccacggcc aatatgtatc
      181 gcctgggctg ccgcacgccc gagatcgagg ccgcctgccg caagcccagc tgcgtctacc
      241 ccggcatctg ccagaacctg acgaccgacc acgacccttt gatttcgatc tacaaaaagg
      301 gccggggcat caagggcatc aagaagattt tgatcggctc gggcctgcgc tacgacctgg
      361 cgattcagtc gcccgaatac gtgaaggagc tggtgcagca ccatgtgggc ggctacctga
      421 agatcgcccc ggagcacacc gaggccgggc cgctgaccaa gatgatgaag cctggcattg
      481 gcaactacga caagttcaag gtcttgttcg agaagtacac cgccgaggcc ggcaagaagc
      541 agttcctgat cccgtacttc atcgccgccc accccggcac cagcgacgaa gacatgatga
      601 acctggccgt gtggctgaag aagaacggtt ttcgcgccga ccaggtacag accttctacc
      661 ccagcaccat ggccacggcc acggccatgt accacacggg catcaacacc ctgaagggcg
      721 tgcaccggga tgaacgcggc gagaaggtgg acgtggtgcg cggcgagaag cgccggcgcc
      781 tgcacaaggc ctttttgcgc taccacgacc cggccaactg gccgctcctg cgcgacgcct
      841 tgaaggccat gggccgggcc gatctgatag gcaatggcaa gcaccatctc atccccacct
      901 accagcccat gggcaagcag tgcgccagca aggacaaggg caagtacctg gtgataccga
      961 ctatggtgct ggaccaggcc cgcgccgacg ccgccaagaa gccccaggcc ggggggcagc
     1021 agggaacggg cggccgcgcc gcggccaagc cagcggccaa aggccgtgcc acgccccagc
     1081 ccaggccggg ccagatcctc agccagcaca cgggcctgcc gccgcgcatg cagcgttgag
     1141 tttgtggtga aatgggtctg tagcgcgcta tggctgcgcg ctacaagcta ttaaatcggt
     1201 agtaaatcgg gtcgtactac cagcacgccc aggcccgcgc aatgcagcag cacggtggcc
     1261 cagtagacga cgcgaaactc ctgcttgctc gacttgtggc gtagccactg ctgggcccac
     1321 caggcggcgg gccagccgcc cagcagggcc agcaggtgca gctgcttttc gctggtgcgc
     1381 cagccgccgc tttgagcagc gctcttgtcc agcttgtagc tggcatacgt gaccaggttc
     1441 agcgccgcca tggcgcccag cagccagagc ggaaaacgct gcgcccagct gccccaggcc
     1501 agcagggcca gccaggcgag catcagcccc atggccaggc ttgcgccggc cggtgtgttg
     1561 tcacgggcct ggggcgcggt gcggcgtcgg gttgccggct gggcaggggc agggcgccca
     1621 gcccgggcgg gtgcgggcgc tgccgcccgg gcgccaagcg ggcgcactgc catggcgcgc
     1681 ggacccttgc cgccaacctg gatctcctcg aactccaccc tcatgccctc ggcgggcggc
     1741 gtggcgccgc gatagtcgcg gacgtgaaag aacacgttgg agctgtgcgc cccctggatg
     1801 aagccaaagc ccttggctga gtcccatcgg cggatgatgc cttgtgtgtg catgtcaaag
     1861 ccgtaaaaaa agccgcccgc aggcggctcg agttggagta ggggcgctca gatcaccttg
     1921 gcaatcgcct ggcagacata gtcaatgttc ttgctgttga gcgcggccac gcagatgcgg
     1981 ccggtgtcgg tgccatagac accgaactcg ctgcgcaggc gcagcatctg ctccttggac
     2041 aggccagagt agctgaacat gccgatctgc tcggtcatga acgacatgtc ctgcttgacg
     2101 ccggcggcct tcaggccgtc caccagcttg ctgcgcatgg ccttgatgcg tacgcgcatt
     2161 tcgcccagtt ccttttccca cagcgcgcgc agctcggggg tattgagtac cgcagccacc
     2221 acggcgccgc cgtggatggg cgggttgctg tagttggtgc ggatggcgat cttgagttgc
     2281 gacagcacgc ggtcggcttc ttccttgctc tggcacagca ccgacagggc gcccacgcgc
     2341 tcgccgtaga gcgaaaagct cttggagaac gatgtcgaga cgaagaaggc caggccggcg
     2401 gcgacaaact tctggatcac cgcgccgtcc tcggcaatgc cgttgccaaa gccctggtag
     2461 gccatgtcca ggaagggcgt caggccgcgt gccttgacca cggcaatcac ctgatcccac
     2521 tgcgccggcg tgatgtcata gcccgtgggg ttgtggcagc aggcatgcag cacgacgatg
     2581 gtgccggact cggcggcatt caggctggcc agcatgccgt cgaagttcac gccgcgcttt
     2641 gccgcgtcgt agtaggcata gctttcgacc gtgaagccgg cgttggtgaa cagcgcccgg
     2701 tggttctccc agctggggtc ggagatcagc accttggccg ccgggctgat cttcttcaaa
     2761 aagtcagcgc caatcttcag cccacccgtg ccaccgatgg cctgcacggt ggccacgcgg
     2821 ccggtcttga cgggttcgga gtcggcgcca aacaccaggc ccttgacggc agcgtcgtag
     2881 gcggcgatgc cgtcgatcgg caggtagccg cgcgcggcgg gcttgtccat catggccttt
     2941 tcggcggcct gcacgcaggc aagcagcggc agcttgccgt tgtcgtcgaa gtacacgccc
     3001 acgcccaggt tgaccttggc ggggttggtg tcggcgttga attgttcgtt cagacccagg
     3061 atggggtcgc ggggggccat ttcgacggcg gtgaacagag acatggagag tccttgaggg
     3121 gaaatcggga gatatgtgcc tggcggcatg gccccatgca tgtttcgcat tcggggcagc
     3181 ccgccatttt aacggccgat accttgcgcc gttgaccgtg ccgacaggtc atcagccctg
     3241 tgccagcagc ctggtcgcct gcgaggcggc gatccactcc tcgttggtgg gctccacgcc
     3301 cacgagcacg cggctggcgc tggtggagat cacggccgcg tggctggcgt tggcgtcctg
     3361 gtccaggttc aggcccagcc agccgagctg cacgcagacg cgccggcgca gctcggcgtt
     3421 gttctcgcca atgccggcag taaagaccag catgtccagg ccgccgagca cggcggcgag
     3481 cgcgccgatc tcgcgcacga tgcggcgcac atacagcgcc agcgcggtgc gcacgcgctc
     3541 gttctcatgc tccagcggca gcagcacgcg cgggtcgggc gacacgcccg acacacccag
     3601 caggcccgag tcctggtaca gcatgcgtgc cacctcgtcc agcgacagct tctctatctg
     3661 catcaggtag atcacggcgc ccgggtccag ggcgccgcag cgtgtgccca tcatcaggcc
     3721 gtccagcgcc gagaaaccca tggtcgtggc cacgctcttc aggccctgca tggcgcacag
     3781 gctggcgccg ctgcccaggt gggcgacgat ggtgcgcccg cgcgcggcgt cgccatggcg
     3841 ctcgggcagg gcgtggccca tgtagtcgta ggacagacca tgaaagccgt agcggcgcac
     3901 gccgcgctcc caggccgacc agggcagggc gaacatctgc tccacgggct cggccgtgcg
     3961 gtgaaaggcg gtgtcaaaaa cggccacctg gggcacgtcg gggtgctgct gcatcagcag
     4021 gctcatggcc ttgagcggaa acggctggtg caacggcgcc agtggtatgt agccgcgcag
     4081 ctcgtcgatc acctgctgcg tgaccagggt gggcgcaaag taatgctggc cgccatgtac
     4141 cacgcggtgc gccacggcgc acgggcgctg gccctgcaag cgcttgagca cgcgctcgcg
     4201 tatcagcgcc agggctgcgg tgttggggtg ctcggtgtcc agcgtgatcg gctggggcgg
     4261 ctggccggcc tcgtcaaagg tgggggtggg cccgcctatg ccctgcacct tgccgctcca
     4321 ccgcggctgg cgtggcatgg ggatagtggc ggcgtcgaac agcgcgaact tgatgctcga
     4381 tgagccgcag ttgagaacga ggatgacgtc actggccatg gctgtgtatt ccctattcaa
     4441 tgcaatcacg gtggcgtgac gcggtagcgc tgcgccagca tcagggcgat ggcgcaggag
     4501 gccagccgcg tctggcgcga gtcggcccgg ctggtcagca ccaccggcac gcgcgcgccc
     4561 atgacgatgc cggcgctgtc ggcgccgccc aggtattcca gctgtttggc cagcatgttg
     4621 ccgctctcca ggtcgggcac caccagcacg tcggcgtggc ctgccacttc ggactggatg
     4681 cccttgacgc gcgccgcctc gggcgagacg gcgttgtcga aggccagcgg gccgtccagc
     4741 aggccaccgg tgatctggcc gcgatcggcc atcttgcaca gcgcggctgc gtccagcgta
     4801 gcgggcatgc gcgcgttgac ggtctccact gcggccagta tggccacgcg gggctgggcc
     4861 acgccgatga catgcgccag gtcgatggcg ttgcgcacga tgtcggcctt gtcgtccagc
     4921 gtgggggcga tgttgatggc ggcgtcggtg atgatgaagg ggcgcgggta gctcgccgtc
     4981 tccatgatga agcaatgcgt caggcgccgc tcggtgcgca ggcccacgtt gggcgcgagg
     5041 atggcgccca ggtattcgtc gctgtgcagg ctgcccttca tcagcgcctg cacctcgccc
     5101 ttgccggcca tgcaggcggc ggctgctgct gcggcgtggc tgtgcggcgc gtccacgatc
     5161 tcacagccgg caagtgatat gccgccctcc gccgccacgg cctgcagcct ggcctgcggg
     5221 ccgaccagca ccgggatgat gatgccggcg tcgcgcgcgt ccatggcgcc gcccaggctg
     5281 agcgcgtcgc aggggtggac gacggccatg cgcaccggct ccaggttgcg cacatgggcg
     5341 atgaggcggt cgatgccgcg cagttgctgg ccgttggcgg gcgcagggtt ggtggaggtg
     5401 atcatgtgct ctgcctgcct tttctcttgc tggccgcacg cttcgggggc tgcacggccg
     5461 gtggggtctc gggtgtgggc agcgctggct ggccggcctg cgctgcctgc tcgaacaact
     5521 gccgcatggt atgccagcgc gcggcctctt cgccctccag cgcattgccc gcctgtagca
     5581 gggcgcccag gtgcagtgcg gcggcctgta tgcgctcggc gggcgcgcgc gccagcagcg
     5641 tgggcagggc tgcgatggcc tcggggccgg caatgtgcat cagcaatgcc tggcggcgca
     5701 ccaggttgcg cgcgccctcg atgtcaaggc ggctcgccag ctcggtctgg cgcaacagca
     5761 acgcgcgacg gtactggcgc tcgtccgctt cgcgccgtgg ttgcagcacg aatatcaacg
     5821 cccgtaccgt cgcttcgggc aggccgccct gctcgatcga cacgcgcaac ccgcccagcc
     5881 gttgcgccat ccacgcgcgg tgctcggggg tgtcgccagg atgcggccgc aacgccgcgg
     5941 ccggatcggc ctggcccgcc agcgcctgca ccagcggatg gctgtagatc gcctcgaaag
     6001 ccctctcctc ggcctcgtcg cgcgattggc tgaacacctg cagcaagtgc gtgatcgcgt
     6061 ccgagcaggc ctcttgccaa ctccaccacg ggttgtcggc gcccaccggc ttgcgatcgg
     6121 cgcgcacgga ttcggcctgc tgttcgacca caggcgcgag caggttgcgg tcggaccaca
     6181 tctcgtagcc gatgcgcagc gggtgccagg cgcgcagcgc gtcggccagc tgcggggtga
     6241 ccgcgccccg aacccagggc tgaaggtagc gacggtacag cgccaaattg acctgcgaga
     6301 catgcgctgc agcggcaaag cgccggtcgc ttgcggccac ctgcacggga tcgggggaca
     6361 cgatggcgcg cacatcgtcc agcgtgcgct ggtgcacggt catcaggtag tcaccgggca
     6421 cgatctcggc gtgcgccatg tccggctcct tgtcctgcac gctgagctcg aacagaccgg
     6481 cgggcagcat gtcgatcagg tcgatgttgc tggtgaactt ggcgtgctct ttgcggccga
     6541 ccgcgcccga aacgaagatg ccgaggtggc cgatgctctg gtgcgtcgca tagacgatgg
     6601 tctgatcatg gcccagcagc tcgtcgtcgc tgcgatagag gtcggtgatc cagcctagcg
     6661 cctgcggtgg cggagtgatg ttgtcgccgt aggagcagaa caccacgatc ggcgaggtga
     6721 tgttgcgcag gtcgatgcgc acgccgtcgc tggtaaccag ctcggccgag ctcagcttgt
     6781 tgccgatgaa caggtcgtcg acgatggtct gcatctccac gtcgttgagg cagacgtggc
     6841 cgccccagta tttctcgaag gcaagaaagc gcggtccttc ggtgtcgacg ttcgcataga
     6901 catggtgctt cttctcccac agggtgttgg ccgggttgag gttctcgaag ttctgcacca
     6961 gccaggcgcc gtcgaacttg ccggcgccga tgtcgctggc cagcgcggtc agccagctgc
     7021 cgccggtcag cccgccgccg tagcgcatcg gattcttgcc gcgccagccg gcccagtatg
     7081 acaagggcgt gccggccagg atgatggggc cgaacagctc gggccagacg gccgcggtca
     7141 tcatgatctg ccagccggcc tggcaattgc cgatcaccgc aggcttgccg tgcgcgtcgg
     7201 ggtgcagctc gatcaccttg cgcagaaaag cggcttcggc gcgcatcacg tcctcgaccg
     7261 tctgccccgg gatcgggttg ggtgtgaagc cgacgtagta gcaggggtgt ccggcagcca
     7321 gcgcggcacc gatttcgctg tcgggcttga agccgccgat gcccgggcca tggccggcac
     7381 gcggatcgac gacgacaaac gggcgcctgg tctcgtcgct ggccacgccc ggctgcggct
     7441 gtatgcgaac cagcccgtaa ttgaccgggc gcggcagcgt gtcgccgcgc atcaccagct
     7501 cggccgcgaa gttcagcacg ttgggcgcgg tttcctgcat gtgcgcgcgg tactggttgc
     7561 cgcgcgctcg catgatgtcg gcgtaaagca ccgcgcgctg catggcgtcg atgccgtagt
     7621 ccagggtgtc gcgggtgaag gtcgatgggt tgaacagcaa gacgcaaatc ctttcttcaa
     7681 tacttaggtg ggtcgccgtt catggcgacg ttgcttgcgg ccatgaagcc gaccacgctg
     7741 ctgacatcct gccggcataa ggatgcgaca ggatgacggc gccaactctc caaattcgga
     7801 tgaaaatcag ctatagccct tatccatcaa gcgcagcatg ctattaaaaa agcatacgca
     7861 agcccaaagg agggcgagtg gccatccgaa acgcgttgcc actcaggctc tcaggcatgg
     7921 cttacttctg catcacatag gtgcctgggg cggcgcccag cgtttttccg ctgccgctct
     7981 tgccgcccat gggcgggggc gccacctggg cgctggcgtt gtgcgccagc caggcgctcc
     8041 aggccggcca ccacgagccc tcatgcactg gcgtctcggc ctgccactgg gcggggttgc
     8101 ggtagcgggc acctgccttg cgcgtggcga tctggtaact gcgcctgggc cgccccggct
     8161 cgctgacgat gcccgcgttg tggccgccgc tggtgaggac gaagctgatg tcggagtcat
     8221 tgagcaggtg aatcttgtag accgagcgcc atggcgccac atggtcgcgc agcgtgccga
     8281 cgacgaacag cggcacgcgg atgtcggcca ccgtcaccgg ctggccatcg acctgccagc
     8341 ggccttcggc caggtcgttg ttcatgtaga ggtggcgcag gtattcgctg tgcatgcgat
     8401 acggcatgcg cgtggtgtcg gcgttccagg ccatcaggtc caccagcgag ccgcgctcgc
     8461 ccagcaggta ttcacgcaca cgccgcgacc agatcaggtc gcgcgagttg agcatctgga
     8521 acgaggcggc catttgccag ccttccaggt agcccttgcg ccacatgcca gcctccagcc
     8581 aggcgagctg gctgtcgtcg acaaacagct tgacctcacc cgattcgcgg tagtcgatct
     8641 cggatgccag cagcgtcagg ctggccaggc gctcgtcgcc gtcgcgcgcc atggccgccg
     8701 ccgcggtggc cagcagggtg ccgcccaggc agtagcccac ggcctgcacc cggtgttttg
     8761 gaacgatggt gcccacggcg tccagcgccg ccatcacccc cagctgccgg tagtcgtcca
     8821 ggcccaggtc gcggtcgtcc gcgtcggggt ttttccagga gatgatgaag acggtgtggc
     8881 cctgagcgac caggtagctg accatggagt tgtggggcga caggtcgagg atgtagtact
     8941 tcatcaccca cgagggcacg atcagcactg gctcggcccc caccttgcgc gtggttggcg
     9001 cgtactggat gagttcgatc aggcggttgc gcagcaccac cttgccgggc gtgaccgcca
     9061 cgtccttgcc gacttcgaac ccctcggcgc cagccggccc cttgccggtg agctggcgct
     9121 gcgcgtcgtc caagaaattc atcgcgcccc gccatagatt gaggccgccg ctgctggcgg
     9181 tggcgcgcag cacttcgggg ttggtggcaa tggcgttggc gggcgacatc atgtccagcc
     9241 attggcgcgc ggcaaattcg accattttct cgtggtgctc ggacacgccg cgcacgtcgg
     9301 tggtggcctc tttccaccat tgctgctgca gcaaaaagcc ctgctggatc aggttgaacg
     9361 gccactgctg ccaggcggga tcggcaaagc gccggtcatg ctccagcggc tcgacgcagg
     9421 cggggcaact ggcctgcagg ccggactgct gcgcgtagcg cgcaaactgc cattgcttgc
     9481 gccaggcgtc cagccacagg ctgcgctgct tgccaggcga cagcgccagg tgcatggacc
     9541 agtcgaacca ggccagggca cctgcggtgg gcgcagcccc ggccgtcagc cgcgcccgcg
     9601 cggcgtaggc caggttgtcg atcatgtcca gaaagtcccc cggcgcctgg gctcgcggct
     9661 ctggcgcctg gggttgcgaa gtgacaaggt cgttcatgtg tcgatttcct tgggtatggg
     9721 gccctgcgaa agcggggcag ggcccgcacg gatgcagcgt ctcaggcgtt catgtcgagc
     9781 acgatgcggc cctcgatctg gccggcatgc atgcgcgcga agatgtcgtt gatgttctcc
     9841 agccggtcgc tggcaacggt ggcggccacc ttggcctgct cggcaaacgc cagcgactcc
     9901 tgcagatcca gtcggctgcc aacaatggag ccgcgcacgg tgatgccgtt cagcaccatg
     9961 ccgaagatgt ccagcggaaa gtcgcctggc ggcagtccga ccagcgacat ggtgccgccg
    10021 cggcgcagca tgccggtggc ctgtgcgaag gccttgggcg ataccgccgt caccagcgca
    10081 ccatgggcgc cgccgatttc cttcttcaga aacgccgccg gatcggtgtt caaggcgttc
    10141 acggtcaccg tggcgcccag gcgctcggca agcctgagct tggtgtcgtc cacatccacc
    10201 gccgccacgt tcaggcccat ggcccgggcg tattgcaccg ccatatggcc caggccgccg
    10261 atgcccgaga tcaccaccca gtcgcccggc ttggtgtcgg tcaccttgag ccccttgtag
    10321 accgtgacgc ccgcgcacag caccggcgct atctcgacaa agccaacgtt ggccggcagg
    10381 tggcccacgt agtcggcgtt gcccagcgcg tattcggcaa agccgccgtt gaccgagtag
    10441 ccggtgttct gctggctctc gcacagcgtc tcccagccgc ccaggcagtg cgtgcaatgg
    10501 ccgcaggccg agtacagcca tggaatgccg acgcggtcgc cctccttgac atgcttgaca
    10561 ccggcgccca ccgcggcaac aaagcccacg ccctcgtggc cgggaatgaa gggcgggttg
    10621 ggcttgaccg gccagtcgcc ctcggcggcg tgcaggtcgg tgtggcagac gccgcaggcc
    10681 tcgatcttga ccagcacgtc acccgcacct gggcgcggca ctggcacttc ttcgatggcc
    10741 agcggctggt cgaaggcgcg tacgacggcg gctttcatgg tcttgttcat cacgaaactc
    10801 cttttcgggg ggtgggtgga aactcaacag gtgttgcggc gggcgtatca ggccatttgc
    10861 gcgatggtct tgcgaaagcg cgccagcgac agcgcaaaca gcaccgcgcc gatcagcgcc
    10921 atccacagaa agggctgcca cacgacgtcc agaccggcgc cgcggtagag gatggcctgg
    10981 ccgatctcga cgaagtgcgt ggtcggcgcc gccagcatga tggtctgcac caattcgggc
    11041 atgctttccc gcggcgtggt tccgcccgaa agcatctgca gcggcatgat ggtgagcacc
    11101 agcaacatgc cgaactgggc catgttgcgc gccagcgtcg ccagaaaaat gcccatcgac
    11161 gtggtggcaa acaggcacag cgccgccccg gtgaagaaca gcggcagcga cccctgcacc
    11221 ggcacctgca gcatggcgcg caccaccagg ttgagcgaca gggcggcggc agccagcacc
    11281 accagtccca tggcccagat cttggacaac atgatctcgg tcggcgtcac cggcatgaca
    11341 agcaggtgct cgatggtgcc gtgctcgcgc tcgcgcagca gcgccgctcc ggtcaggatg
    11401 atggacagca gggtgatctg gttgatgatc tgattcaggc tgccgaacca gcttttctcc
    11461 agcgccgggt tgaagcgcgc gcgcacggcc agctccaccg gcgcggccgt gccgacgcgc
    11521 tggcgctgga cgaactcgcc cacctcggcc agcacgatct gctgcacatt gccgctgccg
    11581 gtgaaggcct ggctcatgcg cgtggcgtcg acgttgagct gcagctgcgg cgcgcgccca
    11641 gccagcacgt cccgctgcaa gccctggggc aggtgcaggg agaaggtgta caggccggcg
    11701 tccatgcccc ggtcggcctc ggtcatggag acgagccgcg gcgcaatgaa ctgcggcggg
    11761 tagaacgcgc tcacgatgcg ctgcgatagt gccgagtcgt cctcatcgat gatcgcgatg
    11821 ggtacgttgt gcaacgtctc tggcatggcc gtggcggcgg tgtagaccga gccggtgaac
    11881 acccagacga tgagcaccag catcattgga tcgcgccaca ggctccacag ctccttgacg
    11941 cccaggcgat agatgttgac gaggtggcgg atgctggagc gcgcgcggtt catctcaacg
    12001 ctcctgcttc ttgagcagca cgatggcggt gccaatgatc accggcaccg acagcagcat
    12061 gggccagaag gcgcccgaca ggtcggcaaa gtccagcgcc ttgctgaaca ctccgcggct
    12121 gatggtgatc atgtgggtgg ccgggtagac ctggccgatc agcttgccgc tgccctccag
    12181 cgaggacacc ggatcgatca gcccggagaa ctgggtggcc ggaatgatgg tgccgatcat
    12241 ggccagaaac atcgccgcga tctggctctt ggtcaccgtg gaggccagca ggcccatgcc
    12301 ggtggagaca aagccaaaca ccagcgcggc cagcgtcaag gtcacaaagc tgcccttgac
    12361 cggcacaccg aacacggtca ccgccagcaa ggccatcagc aggaagttga ccatcgccag
    12421 gccgacgtag ggtagctgct tgccgagcag aaactcactg cgggtgaccg gtgtgacgta
    12481 gaggttggtg atcgagccgg tctccttctc gcgcaccacg gccagcgcgg tcagcatggc
    12541 tggcagcatc aggagcagca tcggaatcac tgccggcacc atggccggca ggcttttcac
    12601 atccgggttg tagcgaaagc gcgtctccac ctgggcggtg ccggccgtgc gcacgcccat
    12661 ggccgcggcc tgcgtggcca gccagctctg gtgcatgccc tgcacatagc cccgcacggt
    12721 ttcggcgcgc gtgggcatgg cgccatcgac ccaggcgccg atctgcacgc catggccgcg
    12781 cagcgcgtcg cgcatgaagc cctggggtat ctcgatggcc agcgacagct cgccgctttg
    12841 catgcgccgg tcgagttcgt cgtagtctgt gagcgcgggt cgctcgacga agtagcgcga
    12901 tcccgacaac tgcagcgcat agtcgcggct gagcgtggtg ttgtcgcggt cgagcacggc
    12961 gtagcgtaga tcctgcacgt ccatgctgat gccaaagccg atcacgaaca tcagcaagat
    13021 cgagccaccc agcgccagcg tcaggcgaat cgggtcgcgc cgcagctcca gcgcctcgcg
    13081 ccacaggtag ctgagcagac gctgcaagct gaatggggtg ctgcgtgcgg gcgccgtggc
    13141 aacttcttgg gtgcttgctg ccggggccga aaccggcggc gcatcctgcg cctcctgcgc
    13201 gccgccctcg gcttcgacca ggtagccgat gaaggcttct tccagcgtct tcgcaccgcg
    13261 cttttgcacc agcttggccg gcgcgtcgct gtcgagcacc ttgcccgcgt gcatcatcga
    13321 catgcggtcg cagcgctcgg cctcgttcat gaagtgcgtc gagatgaaga tcgtcacctg
    13381 gtcgcgccgc gacagctcga tcaacagccg ccagaacgca tcgcgcgcca ccgggtccac
    13441 gcccgaggtg ggctcgtcga ggatcaagag ctcgggctgg tgcaccatgg ccacggccag
    13501 cgacaggcgc tggcgcatgc ccagcggcag gctagcgggc aggctgtcca tggcgccggt
    13561 gaggtcaaaa cgctcggcca tttcctgcac gcgctgcgcg atatgggcct cgggcacgtt
    13621 gaacagctgc gcgtgcagca cgaggttttg ccgtaccgtg agctcgccat agagcgaaaa
    13681 cgcctgtgac atatagccca cgcggcggcg ggtgtcgatg tccttggggt cgacctcatg
    13741 gccgaacagc caagcctggc cttcgctggc tggcagcagg ccggtgagca tcttcatggt
    13801 ggtggatttg ccgcagccgt tggagccgag aaagccaaaa atctcgccgc ggcggatgcg
    13861 aaacgacacc tgatcgacgg cgacgaaatc gccaaagcgc atggtgagat tcttggcctc
    13921 gatcgcgatg tcgccggcat cggcctccag aggaggaatc gtcaccggcg catggccgtg
    13981 cttcttctcc ggtggcaaca gtgcgatgaa ggccgcctcc agcgagtcgg tcgccgtgcg
    14041 tgcgaacaac tcctgcggcg taccggtggc cagcacgcgg ccgtcgtcca tggccatcag
    14101 ccagtcaaag cgctcggctt cgtccatgta ggcggtggcg accagcacgc tcatttgcgg
    14161 gcgctggctg cgaatgcgat cgatcagatc ccagaactgc gaccgtgcca gcggatcgac
    14221 gccggtggtt ggttcgtcga ggatcaacag atcggggtcg tggatcagcg cgcagcacag
    14281 cccgagcttt tgcttcatgc cgcccgagag cttgcccgcc gggcggtcga gaaaccggtg
    14341 caggccggtg gagcgggtga ggtcatcgat gcgccggcgc cgctcgggcg catcgtggcc
    14401 aaacagtcgc gcgaagaact gcaggttctc ttcgaccgac aaggtggggt agaggttttt
    14461 gcccaggccc tgcggcatgt aggcgatgcg cgggcagacc cggttgcggt ggctgcgttc
    14521 gcgcatgtcg ccgccgagca cccacaccgt gccgtcctga atcttgcgcg cgccggcgat
    14581 cagcgacagc aggctcgact tgcccacgcc atcgggcccg atcaggccga ccatgcagcc
    14641 cgctggcagg gcctggtcga tcccggcgag agcgcaccgc ttgccgtagt gcaggccaac
    14701 gccgctgagc cggatgaccg gttcgagctg tgctgaggtc accatgaaat cagctcccac
    14761 ccacggccgt tgtacgcgga tccagcgcca gtgaagccgg ccactgggcg ttggagtcca
    14821 gcttgagcca ggcaacgccc ggcactccgg tcttgacctg ctcgatgtag cgctgcagca
    14881 gctgcggcgc gatctgcgcg cgcacgcgga acatcaactt ctggcgctcg ctggccgtct
    14941 ccaccgtctt gggcgtgaac tgcgcctggc tggcgacgaa tgacacctgg gccgggatca
    15001 ccgtgccagg catggcgtcg agcacgatcc gcacctcgct gcccagcgcg atgcgcccgg
    15061 ccacggtttc gggcacgaag aaggtcatat aaacatcgga tagatcgagc agattgagca
    15121 cgcgcccgcc cgcgcccagc acctcgcccg gttgcgccac gcgcacctgc acgcggccgt
    15181 cgcgcggggc cgtcaacacg gcatctttca tgtcggcgtc aatgcgctcg atgctggcct
    15241 gcgcggcgtc gacctgcgcc ttggcgccgg tgacctgggt gcgggccgct tcggtggcgg
    15301 cgcgtgcggc atcgacctgc gccttggccg ccgtcaccgc cgcctgcgcg ccgcgcatgc
    15361 gggtgcggtc gtcgtccagc tcctgctgcg acgaggcgcc gtcgcgcgac aggtcggtgg
    15421 agcgtgccag gcgtcgctgg gcggcatcca gctcgctctc gcgcagcagt acctgggcct
    15481 gcatggcgcg caggtcgctc tcgcgcagcg ccacttgggc atgcgccgtg gccacgccat
    15541 gctgggcctg tgcatggcgc gccacggcct cgctgcgctg ggcctgcagc gaggccatgt
    15601 ccatgcgtgc cagcacgtca ccggccttga cgaaggcgcc ttcggccgcc agcacctcgg
    15661 ccaggcggcc cggcagcttg gtggccacgt cgatctcggt ggcctcgatg cggccgttgc
    15721 cgtgcacgaa gccttcgccc gggccctggt cgcgcgtggc attccaggcc atgtaagcgc
    15781 cgccagcgag cagcgcgatg gcggcaggga caagccattt tttcaatgtt gcgttcatgg
    15841 tgtcattcct cggatttctg gagcggcaag ggaagcgggg cgagtgtgcg cgagccgccg
    15901 cccagcgccg cgtacaggcg cagccgcgca gtcagcaagg catgctcggc ctgtaccacc
    15961 tgctgctgcg tggcgagcag gtcgcgttcg gcatccagca cctcgaagta gcgcgccgcg
    16021 ccggcctcgt agcgccggcc ggccaggcgc gcacgctcgg tcagcgtgtc ctcggtggcg
    16081 cgcagggtct gcaattgctc ttgcaaggac tggcctgcgg caagcgcatc ggccacgtcg
    16141 cggaacgcgg attcgatggc ctgctggtat tcgttcagcg cctgttcgcg ctgcacctgg
    16201 gcgagctcca ggttggcctg gcggcgcccg ccatcgaaga tgggcagcga cacgctgggc
    16261 gcgaacgtcc aggcgcggct gccggaggca aacaggccat cgagatcagc actggccgtg
    16321 cctgccatgg tggtgagggc gatgcgcggg aagaacatag cgcgcgcggc gccaacgcgg
    16381 gcgttggcgg cctgcagctg gcgctcggca gcgacgatgt cggggcggtt ttccagcaac
    16441 tgcgagggca ggccgggcgc cacgggcggc agcggtgcca gcgcggacag cggttgcgtc
    16501 gccggcgcca ggctcaggcc tggctcaccc accagcaggc ccagcgcatg cagctgcgtg
    16561 ctgcgctgca gctgcaactg cgtcaccagg gcgctggcct gttgccacag cagctccacc
    16621 tgcacgaagt ccagtttgga tgacgacccc acctcgactc ggcgcgcgaa gcgtcggcgc
    16681 gcctcggtgc gcgtggccag cgcctgatcg gccagctgaa tgcggtggtc gagctcgcgc
    16741 accgagagcc agccgtccgc cacctgggcg acaagcatca gggtcgtggc gcgttggccg
    16801 tcggcgcttg ccaggtaggt ttgcagtgcg gcttcgttca tgttgcgcac gcgaccccac
    16861 aggtctatct cccagctggt caagcccacg cccagctggt gttggctgga aatgatgggc
    16921 tcgcgtgtga agttcaggtc gcctggcatg cgtgtgcgct gcgaatccag gcccaggccc
    16981 actgccggca cgcggtcggc gcgcgaaatg ccataggcgg cacgcgcctg ctccacgcgc
    17041 agcatggcgg cgcgcaggtc acggttgttg gccagggcct tggtgatcag cgcttgcagt
    17101 tcgggttcgg tgaagtagtc gcgccaggcc agctcgccga cctggctcgc gtcggccggg
    17161 cttgcgcttg agaactgggc tggaatctgc gctgcaggag gctggtaccc ggggggcagc
    17221 gtcgtgcagc cggccagggc ccacagtgcg ccggccacca gggcgccggc cggcaggcgc
    17281 tgcgcccagg cgccgcgacc ggcagcagcc gggcgccggc gggcaatgga aaacgggaag
    17341 tcgttcatag gctttgatct ttataacaac cgaccagtcg gtaattaata taccatcgac
    17401 accgctgcag caccagccct gaaagatcac aatgccccac atgagcgata gcgaaatcaa
    17461 cctgcccaaa cgcaggctgc gcctggcccc cgaggcgcgc gtgcccctga tactggatgc
    17521 ggcgctgcag gagttctcgc gccgcggctt cagcgccgcg cgcatggacg acattgcgca
    17581 acgctgcggg ctgtccaagg gcgggcttta cgcgcatttc gatagcaagg acgcgatctt
    17641 caaggcgctg ctcgatcgca ccctgcaccg cacggattgg gcgcggatgc cgcagctggc
    17701 ggccggcgcg ggcacgcgcg ccgtcgccga gtggatcgtt gaccacctgc gggccgcgct
    17761 gctggcgccg gacgtgatca cgctgctgcg gctattgatt ccggagcgcg agcgcgtgcc
    17821 gctgcgcgtg gatgaatgga aaaacctgac ccagctgaac acgcagcacg tgacggcggt
    17881 gatcagcgag aacctgaagg ccagcggcag gcaggacagc gtgctggcgc gccacccctg
    17941 gctggcgctg tcgcccatgg tgcatgtgct gctgtggcat gcggtgtttg gcgagggcag
    18001 cgatcccgat ccagattttc accaggcgca tgtggacatg ctgtgcgagc tgttggcgtg
    18061 atgccgatgc ggcatggggg tggaagtcct gcacggcgcc cccagctgca gtaggcttac
    18121 ctgttcaccc ttctatcgcc atgcatgaag tcaccgcaga gcccgccgcc gtggacgggc
    18181 agttcgtcca gtttcccggc tcgccgttcc agctgttcca gccctatccg cccgccggcg
    18241 accaacccga ggcgattgcc aggctggtgg agggcgtgca ggacggcgag gtgttccaga
    18301 ccctgctggg cgtgaccggc tcgggcaaga ccttcaccat ggccaatgtg atcgcccgga
    18361 tgggccggcc ggccatcgtg ttcgcgccta acaagacgct ggcggcgcag ctgtactcgg
    18421 agtttcgcga gttctttccg aaaaacgcgg tggagtattt cgtcagctat tacgactact
    18481 accagcccga ggcctatgtg ccgcagcgcg acctgttcat agagaaggac agcgccatca
    18541 acgagcatat cgagcagatg cgtctgtcgt gcaccaagag cctgctggag cggcgtgatg
    18601 tggtcatcgt cgccacggtg tcggccatct atggcattgg caaccccgag agctaccacc
    18661 gcatgatcat gacgctgcgc gcgggcgaca ggttgagcca gcgcgatgcc ataggccagc
    18721 tgatacgcat gcagtaccag cgcaatgaac aggacttttc gcgtggcaag tttcgtgtgc
    18781 gcggagacac catcgacgtt tttccggccg agcattcgga gttggccgtg cgcatcgagc
    18841 tgtttgacga cgaggtggag agtctgcagc tgttcgatcc gctcactggg cgcgtgcagc
    18901 agaagattcc gcgcttcacg gtctacccca gcagccacta tgtgacgccg cgcgacgtgg
    18961 tgctgggggc ggtggagacc atcaaggagg agctgcgcga gcgcctggaa ttttttgcca
    19021 aggagggcaa gctggtcgag gcccagcggc tagagcagcg cacgcgcttt gacctggaga
    19081 tgctcagcga ggtggggcat tgcaagggca tcgagaatta ttcgcgccat ctgtcgggcg
    19141 cgcagccggg cgagccgccg agcacgctca ccgactacct gccgccggat gcgctgatgt
    19201 tcctcgatga gagtcatcag atgatcggcc agctcaacgc catgtacaac ggcgaccgct
    19261 cgcgcaagac cacgctggtc gaatatggct ttcgcctgcc ctcggcgctg gacaaccggc
    19321 cgctcaaatt cgaggaattc gaaggccgca tgcgccaggt gatttttgtc tcggccacgc
    19381 cggctgacta cgagaagcag cattccggcc aggtggtgga tcaggtggta cggcccacgg
    19441 ggctggtcga tcccgaggtg gaggtacgcc cggccaccca ccaggtggat gacgtattgc
    19501 aggagattcg cctgtgcgtg cagcagcgcc agcgcgtgct catcaccacc ctgaccaagc
    19561 gcatggccga gcagctgacc gactatctgt ccgacaacgg tgtcaaggtg cgctacctgc
    19621 actcggatgt ggacacggtg gagcgcgtgg agatcattcg cgacctgcgc ctgggggcgt
    19681 tcgacgtgct ggtgggcatc aatctgctgc gcgaaggcct ggacattccg gaagtctcgc
    19741 tggtggcaat tctggatgcc gacaaggagg gctttttgcg cgccgagcgc agcctgatcc
    19801 agaccatagg ccgggcggcg cgcaatttgc acggaagggc catcctgtat gcagaccgcg
    19861 tgaccgactc catgaagaag gccattggcg agaccgagcg tcggcgtgcg cggcaactgg
    19921 cctacaacca ggcgcatggc atcacgccgc gtggcatcgt caagcaggtg cgcgacctga
    19981 tcgacggcgt ttacagcgac aaggccggtc aggaggcgct gcgcctggaa caggaggctc
    20041 agcgccacgc cgagctcgac gagatgtccg aaaaggacat tgcgcgcgag atcaagcggc
    20101 tggaaaaacg catgttggag catgcgcgca acctggagtt cgagcaggcg gcgcgcgtgc
    20161 gcgacgagct ggcgcatctg aaggacaggg tgttcggcgc ccatggcggc gaccaggtgc
    20221 cggcgtagtg acctggcggg tgattacgcg actttccagt ctatttaccc gacagaatta
    20281 cttgggtttg tgttatactc ccacaaacta ctcaactaat cagcccatta cgcacggggg
    20341 cctcggggtg gcggtttcgg ccgcatgcct ctggaggagg gcgcggaggg tgcccaggcg
    20401 tggcctcatg gcgcgccttg tgcgtttctc taaaacgaac acgcctagac cctaggcaag
    20461 gagcttggaa tgcgactcac cacgaaaggc cgttttgcgg tcaccgccat gattgatttg
    20521 gccctgcgcc agaacaacgg ccccgtcacg ctggctgcca tcagccagcg tcagcagatc
    20581 tcgctgtcgt acctggagca gctgtttggc aagctgcgcc gccacgagct ggtggagtcc
    20641 acgcgcggtc ccggtggcgg atatacgctg gcgcgcaagg ctggcgacat caccgtggcc
    20701 gacatcatcg tctcggtgga cgagccgatt gatgccaccc agtgcggcgg caaggaaaac
    20761 tgcctgggcg aggccggccg ctgcatgacg cacgacctgt gggccacgct gaaccagcgc
    20821 atggtcgagt ttctggattc ggtcacgctg caaaagctgg tggacgacca actcgccaag
    20881 ggcatccaga tcgaggacag gcccgttacg cgccgcgcga tttccaccac gccggtggtc
    20941 aagccgatcc gcgtgaatgc gcccaactcg gtgtttgccc tgggcaacgc attcgccaag
    21001 tcctgagagc ctgagccggc cgttggcagg cccaggcctg cgccgggccg tgacccgtat
    21061 taccttgttt gacttctgac tcgcgccagc cagagccctg tacttgagcc agccatggat
    21121 atgacaccgc atttccccat ttacctagac tacggcgcca ccacgcccgt cgatcctcgc
    21181 gtcgtggacg ccatggttcc ctggctgcgt gaacattttg gcaacgccgc ttcgcgcagc
    21241 cacgcctggg gctgggaggc cgaggaggcc atcgagaagg cgcgcggcca ggtggccgac
    21301 ctgataggcg ctgacccgcg cgagatcgtc tggaccagcg gcgccaccga gtccatcaac
    21361 ctggccttga agggtgccgc ccagttctac aagaccaagg gcaagcacct gatcaccctg
    21421 aagaccgagc acaaggccgt gctcgacacc atgcgtgagc tcgagcgcca gggcttcgag
    21481 gtgacttacc tggatgtcaa ggaagacggc ctgctggatc tggatgccct caaggccgcg
    21541 atccgtcctg acaccatcct gatcagcgtg ctgttcgtga acaacgagat cggcgtgatc
    21601 caggacattc ccgccatcgg cgccctgtgc cgcgaaaggg gcatcctgct gcatgtggat
    21661 gcggcccagg ccaccggccg ggtcgagatc gacatggcca gcctgcccgt cgacctgatg
    21721 agcatgacgg cgcacaagac ctacggcccc aagggcgtgg gtgcgctgta tgtgcgccgc
    21781 aagccccgcg tgcgcctgga agcgcagatc cacggcggcg gccatgagcg cggcatgcgt
    21841 tcgggcaccc tgcccacgca ccagatcgtg ggcatgggcg aggcctttcg catcatcaaa
    21901 caagagatgc acgaggtgaa cgccaaggcg cgcgtgctgc agcagcgcct gctcgacggc
    21961 ctgaaggaca tcgaacaggt attcatcaac ggcagcctgg agcagcgcgt gccgcagaac
    22021 ctgaacatga gcttcaacta cgtcgagggt gagtcgctga tcatgggcat caagggcctg
    22081 gcggtgtcca gcggctcggc ctgcacctcg gccagcctgg agcccagcta cgtgctgcgc
    22141 gccctgggcc gcagcgacga gctggcgcac agcagcctgc gcatgaccat aggccgcttc
    22201 accaccgagg aagaaatcga ctacgcgatc agcaccattc gccacaacgt ggccaagctg
    22261 cgcgagctga gccctctgtg ggagatgtac caggatggcg tggacttgag caccatccaa
    22321 tgggcggcgc attgagcgcg ccagagcaga tttgaatcag gagaaataca ccatggctta
    22381 ctcggacaaa gtgattgacc attacgagaa cccgcgcaac gtgggctcgt tcgacaaggg
    22441 cgacgacagc gtgggcaccg gcatggtggg cgcgcccgcc tgcggcgacg tgatgaagct
    22501 gcagatcaag gtgaacgccg agaccggcgt gatcgaggac gcgcgtttca agacctatgg
    22561 ctgcggctca gccattgcct cctcgtcgct ggtcaccgaa tgggtcaagg gcaagacgct
    22621 ggacgaggct gcgcagctca agaacagcca gatcgccgag gagctggcgc tgccacccgt
    22681 gaagatccac tgctccatcc tggccgaaga cgcaatcaag gccgccgtca aagactacaa
    22741 ggccaagcac gacgccgtcg tggaggccgc ctgataccgc catggccatt acgctgaccg
    22801 aagccgccgc ccggcatgtg atccgctacc tggcccgcag gggcaagggg tttggcgtgc
    22861 gcctgggcgt caagaccacg ggttgctcgg gcctggccta caagctcgaa tacgtggatg
    22921 agcaggcacc cgaggacatc gtgtttgaac aacatggcgt gaaggtgctg atcgacccca
    22981 agagcttggc gtatatcgac ggcaccgagc tggattttgt gcgcgagggc ctcaacgagg
    23041 gcttcaagtt cgtcaacccc aacgagcgtg accgctgcgg ctgtggggag agtttccgcg
    23101 tctgaccctg tcgtgaccgc gttgacgacc gcaaccgcca tcgcccgccg cgctggcggt
    23161 tttttattgc catgaatctt caatccgacg atttcgaact ctttggcctg cagccacgtt
    23221 ttgcgcagga tcgcgccatg ctggatgccc gctggaagga cctgcagcgc gaggcccacc
    23281 ccgaccgctt tgccgcgcaa gggccggccg cgcagcgcgt ggccatgcaa tggtcggtgc
    23341 gcatcaacga ggcctaccag cgcctgaggg atccgctcaa gcgtgccgcc tacctgtgcg
    23401 agctgcatgg cgcgccgata cgcgccgagg acaacacggc catgccggcg gcctttctga
    23461 tgcagcagat gcagtggcgc gaggcgctgg acgaggccac cggcgcagcc gagctcgacg
    23521 cgctggagga cgagacgcag gccgcgcgcg gcgccgccct ggcgcgctgc gggcaactca
    23581 tcgacgtccg ccaggattac gcgggcgccg cgcgcgaggt cagatccctc atgttcattg
    23641 cgcgatttgc acgggacatc gatttgcgcc gtgagcaact gggacaatag ggcatctccc
    23701 gattgatctt ccagaggttc accttgtggc ctgctgggac tcttcatgac aaacatccat
    23761 ggcgcttttg cagatttccg aacccggcca gtcgcctgac ccacaccagc gccggattgc
    23821 cgtaggcata gacctgggca ccacgcattc gctggtagcc gcggtaaaga gcggcgtggc
    23881 cgaatgcctg ccagacgccc agggccgggt gctgctgccc tcggtcgtgc gttatctcca
    23941 ggacggcgga cgccatgttg gccatgaggc cgccgcggcg cagacgcagg acccggtcaa
    24001 caccgtggcc tcggccaagc gcttcatggg ccggggcctg gccgacatcg ccgggcgcga
    24061 gaagctgccc tacgactttg tcgctgcaca ggccgccggc ggcatgctga gcctgtccac
    24121 cgtggccggc gtgaagtcgc ccgtggaggt cagcgccgag atcctggcca cgctgcgcta
    24181 ccgtgcggaa gacagcttcg acagcgacat ctacggcgcc gtgatcaccg ttccggccta
    24241 ttttgacgac gcccagcgcc aggccaccaa ggatgcggcg cgcctggccg gcctgaacct
    24301 gctgcgcctg atcaacgaac ccacggccgc ggccattgcc tacggcctgg acaacgccgc
    24361 cgagggtgtc tacgccgtgt acgacctggg cggtggcacc ttcgatattt ccatactgcg
    24421 cctgacccag ggcgtgttcg aggtcatcgc cacgggcggt gactcggctc tgggcggtga
    24481 cgactacgac gcggctcttg ccgactgggt gctgcagcag ctgggcctgc aggcgcatac
    24541 gcccgccgac aaggctgccg tgcgcctggc ggcacgcgcc tgcaagcaag ggttgacgac
    24601 tactgaaact gtagcgttca gcgcttattt ggcgggcgtt gaggccaaat ttgatgttaa
    24661 aagctccgat tttgcggccg tcacggcagc gctcacccag cgctcgctgg ccgccgtgcg
    24721 ccgcgcgctg cgcgacgcgc agatcacacg cgacgaagtg cggggcgtgg tgctggtggg
    24781 tggctccacg cgcatgccgc aggtgcgcga ggcggtggcc gacttttttg gccgcgagcc
    24841 cctgaccaat ctgaatcccg acgaggtcgt ggccctgggc gcggccatac aggccaacca
    24901 gctggccggc aacagcacca gcggcgacct tctgttgctc gacgtgatcc cgctgtccct
    24961 ggggctggag accatgggcg gcctggtcga gcgcatcgtg gcgcgcaacg aaaccattcc
    25021 caccgccaag gcccaggact tcactaccta caaggacggg cagacggcct tggccatcca
    25081 tgtggtgcag ggcgaacgcg acctggtgca ggactgccgc agcctggcgc gctttgagct
    25141 gcgcggcata ccgcccatgg cggcgggcgc ggcgcgcatc cgcgtgacct ttaccgtgga
    25201 cgccgacggc ctgctgagcg tgagcgcgcg cgagcagggc agcggcgtgg aggccagcat
    25261 cgacgtcaag ccatcctatg gcttgagtga tgaagagatc gcccgcatgc tgcaggacgg
    25321 ctttgccacg gccgagcagg acatgcaggc gcgcgccctg gtggaggcac gcgtcgacgc
    25381 cgaccgcctg ctgatcgcca cgcaaagcgc gctcgacgcc gatggcgatg tgctcgccac
    25441 cgatgagcgc acggccatag acgccctgat gcgggcgctg cgcgcagtgc tcgacgcggg
    25501 ggagggcgcg gccagcgtcg aggccgccac ggccgccctg gccaagggca ccgaggcctt
    25561 cgccgctcag cgcatgaacc gcggcatccg ccaagccctt tccggcaaaa acgtcagcgc
    25621 gctttaaccc atttcttttg ccatgcccgt catcaaaatt ctgccccacc acgaatactg
    25681 ccccgagggc gccgagatca gcgcgagcgc cggcacctcc atctgcgagg ccttgctgga
    25741 gcatgacatc aagatcgagc acgcctgcga catgagctgc gcctgcacca cctgccatgt
    25801 catcgtgcgt cagggatatg aaacactcaa cgaggccgag gaagaggaag aagacctgct
    25861 cgatcgtgcc tggggcctgg agccgcagtc gcgcctgtcc tgccaggcca tcctggcgca
    25921 gcaggacgtg accatagaaa tccccaaata cacgatcaat cacgccaggg agaaccattg
    25981 atgacccggc agatcgttct ggacaccgaa accaccggcc tgtccgcgca gacgggcgac
    26041 cgcatcatcg agctcggttg cgtggagctg gtgaaccgca agctcaccgg caacaacctg
    26101 cacctgtacc tgaaccccga gcgcgacagc cacgaggacg cgctcaaggt gcacggcatc
    26161 agcaacgagt ttctgcgcga caagcccaag tttgccgact gcgtgcagga catcctgcag
    26221 tacctgcagg gggccgagct gatcatccac aacgccggct tcgacgtcgg ctttctggac
    26281 aaggagctgg agctcgtcgg tctgccgccg ctggccacgc atgtggacag catcaccgac
    26341 accctggcca tggccaagga gctgttccca ggcaagcgca actcgctcga tgcgctgtgc
    26401 gaccgcctgg aggtggacaa ctcgggccgc acgctgcacg gcgcgctgct cgacgccgag
    26461 ctgttggccg acgtgtacat caacatgacc cgggggcagg acgcgctcct gatcatggac
    26521 gaagcgcctg ccggcgacga cggcatcagg gtcgccgcca tggacctgag ccgcatcgcg
    26581 ctgcccgtgc tgcccgccaa tgaacaggag ctggccgagc acgaagacct gctcacgcag
    26641 atcgacaaat ccagcggtgg gaaaacaatt tggcgaactt ttgctggtgg cgaaaaagct
    26701 atgccataat cgcaggctgt cgcaacaacg acaaacgggt gattagctca gcggtagagc
    26761 actgccttca cacggcaggg gtcaca
//
LOCUS       DAOMTQ010000023        26399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_183_length_26399_cov_26.815252, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000023 DAOMTQ010000000
VERSION     DAOMTQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 26399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26399
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_26399_cov_26.815252"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="ugpE"
                     /locus_tag="PK518_03985"
     CDS             complement(<1..661)
                     /gene="ugpE"
                     /locus_tag="PK518_03985"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HRM48102.1"
                     /translation="MVDRNPWLTFLSHAVLILGVAIVAFPLYLALIASTHQAETIVQS
                     PMPLLPGSHMWENYRDALLGSGKLGSNTNVVRMMWVSFVVAIIITVGKIAISLLSAFA
                     IVYFRFPFKMACFWAIFLTLMLPVEVRILPTYKVVADLGLLNSYAGLSLPLIASATAT
                     FLFRQFFLTVPDELVEAARIDGAGPMRFFKDILVPLSRTSIAALFVIQFIYGWNQYLW
                     PL"
     gene            complement(663..1544)
                     /gene="ugpA"
                     /locus_tag="PK518_03990"
     CDS             complement(663..1544)
                     /gene="ugpA"
                     /locus_tag="PK518_03990"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HRM48103.1"
                     /translation="MEKRVLFRSAWLPWVLIAPQLAIIGIFFFLPAGQAVLQSFQVED
                     AFGMSREWVGLENFRQLWDNPDYLGSFQRTALFSVLVAGIGIVVSLALAIFADRIVRF
                     AMVYKTLLIVPYAVAPVIAGVLWVFLFSPSIGVVTYYLGKLGYGWNHLMNENQAMALI
                     VLASVWKQISYNFLFFLAGLQSIPKALIEAASIDGAGPWRRFWNIQLPLLSPTTFFLL
                     VINIVYAFFDTFGIIDAATQGGPGQSTSILVYKVYLDGFKALDLGGSAAQSVVLMAIV
                     VVLTVIQFRYVEKKVQY"
     gene            complement(1712..3025)
                     /gene="ugpB"
                     /locus_tag="PK518_03995"
                     /pseudo
     CDS             complement(1712..3025)
                     /gene="ugpB"
                     /locus_tag="PK518_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            3054..3296
                     /locus_tag="PK518_04000"
     CDS             3054..3296
                     /locus_tag="PK518_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48104.1"
                     /translation="MKPAMVWPLHDNTVSLSHAENAATTGETRQPAERSNCDIGVMLC
                     VRICAFWRGLWRYREQGYAISRMSARICALHNERRC"
     gene            3352..4581
                     /gene="serA"
                     /locus_tag="PK518_04005"
     CDS             3352..4581
                     /gene="serA"
                     /locus_tag="PK518_04005"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRM48105.1"
                     /translation="MPKTSLDKSKIKFLLLEGVHESAVQVLRAAGYSQIELLPGALEG
                     EELRKKIADVHFIGIRSRTQLTAEVLAQAHKLVAIGCFCIGTNQVDLDAARERGIAVF
                     NAPYSNTRSVAELVLAEAILLLRGVPEKNAVAHRGGWLKSADNAFEARGKILGIVGYG
                     AIGSQLSVLAESLGMQVIFHDVVNKLPLGNARQAAGLADLLGRADIVSLHVPELPSTQ
                     WMMGAAQIAAMKPGAILINASRGTVVEIEPLAQALKDKKLLGAAVDVFPVEPKSNKDE
                     FQSPLRGLDNVIITPHVGGSTQEAQANIGLEVAEKLVRYSDNGTSTTSVNFPEVALPA
                     HPGKHRILHIHRNQPGVLSAINQVFAQLHINIAAQYLQTSDKLGYVVIDLDAASSVQA
                     LEKLAQVDGTLRCRVLF"
     gene            4774..8700
                     /locus_tag="PK518_04010"
     CDS             4774..8700
                     /locus_tag="PK518_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HRM48106.1"
                     /translation="MNAPIAHAALLAAGTEPVRLREIPYNYTSFSDREIVIRLLGAEA
                     WGVLDQLRRERRTGRSARMLYEVLGDIWAVQRNPYLQDDLIANPQRRKMLVDAMQHRM
                     GEIEKRRTPQEDAGRDRRVGLLVQAANRAITEFDATLVEVTALRKSVAKRLGKHTARD
                     NIKFDGLSRVSHVTDATDWRVEFPFVVLTPDTEAEMAALVAACIALELTIIPRGGGTG
                     YTGGAIPLTWRSAVINTEKLEAMSEVEMRKLPGLDTEVATVWSEAGVVTQRVADAAER
                     GGYVFAVDPTSIEASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVTPDAQWLEVTRI
                     NHNLGKIHDVEVASFELAYFEADGKTAIRTERLDIPGKTFRKEGLGKDVTDKFLSGLP
                     GVQKEGTDGLITSARWIVHRMPAHTRTVCLEFFGSAKLAVPSIVEIKDFMFAEQKRSG
                     VLLAGLEHLDDRYLKAVGYATKSKKGGGKLPKMVLIGDIVGDDQDAVARAAAEVVRIA
                     NSRDGEGFTAVSSEARKKFWLDRKRTAAISKHTNAFKINEDVVIPLPRMAEYTDGIER
                     INIELSLRNKLALCDALRDFLARGDLPLSQQGDAQGIGADVMLGDRVAQAQALVAEVR
                     ALWQGWLDGVEALFPQLQDHSLRASWKSQLQKPLAEIFTGAAFEPIVKETIAIHQRVL
                     KGRVWVALHMHAGDGNVHTNIPVNSDDYEMLQTAHQAVARIMQLARSLDGVISGEHGI
                     GITKLEFLTPEELAPFAEYKRRVDPEGRFNWGKLLRNQELLAQYPQGQNADIAQKHSR
                     TSLRYADLTNAYTPSFGLMGYESLIMQQSDIGAIVASVKDCLRCGKCKPVCSTHVPRA
                     NLLYSPRNKILATSLLAEAFLYEEQTRRGVSSHHWQEFEDVADHCTICHKCFNPCPVK
                     IDFGDVTMAMRNLLVKMDKKSWRPGNKLAMAMLNATSPTTINVLRAGMVNVGFPAQRL
                     AVDVMKAVAKPQVTRPPATVGTAPIKEQVIHFVNKKLPGGLPTKTARALLDIQDKDYV
                     PIIRNPQTSADSEAVFYFPGCGSERLFSQVGLATQAMLWHAGVQTVLPPGYLCCGYPQ
                     RGNGLQDKAEKMITDNRVLFHRVATTLNYLDIKTVVVSCGTCYDQLQGYEFDKIFPGS
                     RIVDIHEYLLEKGITLPAGQGGYLYHEPCHNPMKQGDSMATVRALVGDKVLKSERCCG
                     ESGTLGVSRPDISTQVRFRKTEEIRKGEAQLRASGAVGAQDNVKILTSCPSCLQGISR
                     YENDLSTGLLEADYIVIEMAKQILGENWLPEYVARANAGGIERVLV"
     gene            8697..9140
                     /locus_tag="PK518_04015"
     CDS             8697..9140
                     /locus_tag="PK518_04015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRM48107.1"
                     /translation="MSCVLCDRDGGQLVWRGDKLRVIRADEAGFPAFYRVIWNTHVAE
                     FSDLSAAERAHCMQAVALVEQTLREQLQPTKINLAALGNVVPHLHWHVIARFDWDSHF
                     PAPVWAGAQRARDAAREDELRLRQVTLEAAMRARLGAWTGTRSDD"
     gene            9166..9582
                     /locus_tag="PK518_04020"
     CDS             9166..9582
                     /locus_tag="PK518_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRM48108.1"
                     /translation="MAGLTPSTPTPESITLHEKSRALELAFSDGARFRIPLELLRVCS
                     PSAEVMGHGPGQEVLQTGKRDVTLVDLQPVGNYAIQPSFSDGHNSGIYTWAYLYELGR
                     DQDALWQRYLQRLRDAGLDRDAPMAPKGQKSGCSSH"
     gene            9718..10449
                     /gene="ubiE"
                     /locus_tag="PK518_04025"
     CDS             9718..10449
                     /gene="ubiE"
                     /locus_tag="PK518_04025"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRM48109.1"
                     /translation="MSTTHFGFETVDEKDKARHVRGVFDSVAPRYDLMNDLMSAGLHR
                     AWKAYTVMVANLREGDQVLDIAGGTGDLSLAFSRKVGSTGSVVHTDINEAMLNVGRDR
                     LINLGVVLPTLVCDAEALPFPDNHFDLVSVAFGLRNMTHKDEALKEMCRVLKPRGRLL
                     VLEFSKVAQPLAKAYDWYSFQVLPRLGKLVAGDDSSYRYLAESIRMHPGQDELKALMQ
                     QSGFGHVDYHNMTGGIAALHVGIKC"
     gene            10495..11484
                     /locus_tag="PK518_04030"
     CDS             10495..11484
                     /locus_tag="PK518_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HRM48110.1"
                     /translation="MKLWSVILVAMLSVVHLDADARRMGGGKSIGKQSSNVTQREAAP
                     PAAPAAPMQQNAANSAAPKPAAAPNAAAGAPKKPWGAMLGGLAAGLGLAWLANSLGLG
                     AAFGNFLLIALLALVVMVVIGMVMRARKPAASQAASSPFAFQGAGAPAPVDVQMPRQY
                     NPEKVGNDASARPWEQFSASPQQGGGSMIGSALAGSQNWGVPADFDAEGFLAAAKRNF
                     TTLQAAWDRSDIATLRSMMTDGMLDEIRAQLAEREAQRAGEHPNHTEVVILEAQLLGI
                     EDLGDAYMASVEFSGMIREEPSAGPSPFREVWNMTKPKSGTSGWLVAGLQALQ"
     gene            11543..12100
                     /locus_tag="PK518_04035"
     CDS             11543..12100
                     /locus_tag="PK518_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48111.1"
                     /translation="MATQSPFSFLDGLLQRVLAGPQPPAWLVHELQHRLVLFLNHVLM
                     QEREAMERLTRQRGRVALVQWRVFTMALQVTPAGLLDLAPLGVVPDLRVEVSDSSPLS
                     LAKGALRGDKPAIRIDGDVQLAADINWLVDHVRWDVEEDLARVIGDAPARALAAVAQR
                     AAGALRGFVGARMGQGAGASDRMRL"
     gene            12097..13662
                     /gene="ubiB"
                     /locus_tag="PK518_04040"
     CDS             12097..13662
                     /gene="ubiB"
                     /locus_tag="PK518_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HRM48112.1"
                     /translation="MSRFFRGISIVWVVLRYGLDELVLSSFQHPWLRALTRIITIGRS
                     LDAPRGQRLREALESLGPIFVKFGQVLSTRRDLMPPDIADELALLQDRVPPFDPDIAI
                     ATIERSFRRPLGEIFVSFEREPVASASIAQVHFAVIRDRDGRERDVAVKVLRPNMLPV
                     IDKDLALMRMMAGWVESLSADGKRLKPREVVAEFDNYLHDELDLVREAANAAQLRRNM
                     EGLDLVLIPEIFWDFCHADVAVMERMNGVPISQVDRLRAAGVDIPKLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIQVSLDGSSFGRYISLDFGIVGTLTEFDKEYLAQNFTAFFRRD
                     YKRVAELHIESGWVPASTRVNELEAAIRTVCEPYFDRPLKEISLGMVLLRLFQTSRRF
                     QVEIQPQLVLLQKTLLNIEGLGRQLDPDLDLWSTAKPFLEKWMLDQLGPQRLWRELKA
                     EAPHYAKILPDLPRLLHDYLRQRPHDSRADLQALLAAQQRTNRLLQGIIYGALGFVLG
                     LLAMQLVVRVHLF"
     gene            13701..15152
                     /locus_tag="PK518_04045"
     CDS             13701..15152
                     /locus_tag="PK518_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRM48113.1"
                     /translation="MLIFMVIAYLFVTIGIGLWAAQRVKNTADFAIAGRNLPMYMIIT
                     TTFATWFGSEIVLGVPAKFIQGGLNAVVEDPFGAGMCLILVGLFFAAKLYRMTLLTIS
                     DYYRERYGRVVEIICSLIIMLSYLGWVSAQVTALGLVFNLLSGGSLSIPWGMTIGVLS
                     ILVYTLWGGMWSVAVTDFIQMIILVLGLVILSWFAADMAGGAGKVVDLITSRDMLRFW
                     PEPTWHEMLFFFGAAITMMLGSIPQQDVFQRVMSANTEKAATHGTVIGGSAYILFAFV
                     PMFLVASALLIMPEQAAELLQEDPQKVLPTLVMTRMPLVMQVLFFGALLSAIKSCASA
                     TLLAPSVTFTENIWRQFRPEHISDREKLLTMRISVLVFAAGVLVYSIKMEGTPIYELV
                     SSAYQVPLVGAFVPLVFGLYWKRANTQGAVCAVVLGIGVWVVFMLTPALHEAFPQQLA
                     GLLSAVFGMVAGSLAPQMVRNTRAPHHRVVGVE"
     gene            15223..15507
                     /locus_tag="PK518_04050"
     CDS             15223..15507
                     /locus_tag="PK518_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM48114.1"
                     /translation="MPIYAYQCGTCGHAKDVLQKMSDAPLTDCPACGAQTFTKQLTAA
                     GFQLKGSGWYVTDFRGGAGGTAAAAPAAPDAPKKEAVSAAPASAPASSDA"
     gene            15522..16145
                     /locus_tag="PK518_04055"
     CDS             15522..16145
                     /locus_tag="PK518_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRM48115.1"
                     /translation="MAALRKWLFTGLLVIVPGVITAWVLTWIVSTLDQTLTILPGAWH
                     PDRLLGFHIPGFGVLLTLAILLIVGAFASNFAGRKLVSLGDSLVTRIPVVRSIYSSVK
                     QVSDTLFSESGNAFRTAVLVQWPRDGVWTVAFITGQPSGEVAGYLRDEFVSVYVPTTP
                     NPTSGYFVLMRKSDCVELDMSIDAALKYIVSMGVVAPPDPVALEAAK"
     gene            16249..18057
                     /gene="aspS"
                     /locus_tag="PK518_04060"
     CDS             16249..18057
                     /gene="aspS"
                     /locus_tag="PK518_04060"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRM48116.1"
                     /translation="MAMRSEYCGLVTEALLGQTVSLCGWVNRRRDHGGVIFIDLRDRE
                     GYVQVVCDPDRAEMFKVAEDIRGEFCVRVVGLVRARPAGTTNDKLKSGQIEVLCHELT
                     VLNASVTPPFQMDDDNLSETTRLTHRVMDLRRPAMQKNLMLRYKTAIQVRNYLDKLGF
                     IDIETPMLGKSTPEGARDYLVPSRVHDGQFFALPQSPQLYKQMLMVAGYDRYYQITKC
                     FRDEDLRADRQPEFTQIDCETSFLGEEEIRAIFQQMIKEVFQTQLNVDLGEFPIMAYQ
                     EAMARFGSDKPDLRVKLEFTELTDVMRDVDFKVFSGAANMAGGRVVALRVPHGSVENG
                     GISRGEIDQYTEFVKIYGAKGLAYIRVNELAKGRDGLQSPIVKNIHDAALAEVLKRSG
                     AQDGDLIFFGADKAKIVNDAIGALRLKIGHSEFGKKHGLFEDRWAPLWVVDFPMFEHD
                     EEDNRWVAVHHPFTAPKDGHEDLMDTDPAKCIAKAYDMVLNGWELGGGSVRIHRAEVQ
                     AKVFAALHITPEDQRAKFGYLLDALQYGAPPHGGLAFGLDRLITLMTGAESIRDVIAF
                     PKTQRAQDLLTQAPSPVDEKQLRELHIRLRNPAVVG"
     gene            complement(18123..18707)
                     /locus_tag="PK518_04065"
     CDS             complement(18123..18707)
                     /locus_tag="PK518_04065"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRM48117.1"
                     /translation="MRKTLQILCLLGSLCWGAPAWSAVAIAGVQVQETAIVAGSELRL
                     NGAGIRFKGPFKVYVGSLYASKPIKSLDELIAAPGPKRLSMTFMREVKSSDFGKMLTQ
                     GIEDNVSRQEFPQIIPGMVKISEYFALHQSFSPGDVCSVEWDPAKGLSIWVKGRQQGE
                     PSRDAAFFRALMSIWFGPAPADWKLKEALLGGSP"
     gene            19036..19491
                     /gene="nudB"
                     /locus_tag="PK518_04070"
     CDS             19036..19491
                     /gene="nudB"
                     /locus_tag="PK518_04070"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HRM48118.1"
                     /translation="MAYKIPESVLVVIHTPDLQVLLIRRADAAEDFWQSVTGSKDSLG
                     EALAATAAREVREETGIDAGAPGCRLRDWGLENVYAIYPQWQHRYAPGVWRNTEHVFG
                     LQVPQGTPVVLNPREHTAYAWLDWRAAADRCFSPSNAEAILWLPRFAAR"
     gene            19488..20258
                     /locus_tag="PK518_04075"
     CDS             19488..20258
                     /locus_tag="PK518_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRM48119.1"
                     /translation="MTAFAISELPAEYSGILRVATYNIHKGVQGLGPARRLEIHNLGL
                     AVEQLDADIVCLQEVRRLNHKEAAYFRRWPRVPQAEYLAPEGYDAVYRTNAWTRHGEH
                     GNALLSRWPVIGHQHEDISDHRFEQRGLLHVEVEVQGRRVHAIVVHLGLIPGSRVRQV
                     AQLQRFIEREVPAGAPLVVAGDFNDWGLQIKRMLAGFGLHEYDDAARTFTYPARLPIV
                     QLDHVYVRGLTPLGLQVPRGRIWWRMSDHLPLIAEFRL"
     gene            20268..21497
                     /gene="clsB"
                     /locus_tag="PK518_04080"
     CDS             20268..21497
                     /gene="clsB"
                     /locus_tag="PK518_04080"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HRM48120.1"
                     /translation="MVTPTRPTKPATDHQIQLLEGAREFFPALITEMDAALSDIQFET
                     YIFDCTGTGADVAEALMRAAARGVRVHLVVDGVGTGRLPEPWRTRLRSAGVRIEVYSP
                     LGPLGLLLPRRWRRLHRKLCVIDGCVLFCGGINVLDDFYDPNHGPLAAPRFDFAVRAV
                     GSLVQHASDTMEQLWWRMRAVSDVRHRRLSLALADMRAASAARLAIKRAQSQRRGMRA
                     ALLLRDNVLHRTSIERAYLRAIAQARREVIIANAYFMPGGKLRRALILAARRGVRVQL
                     LLQGRYEYFMQYHAARPVYGALLAAGVEIYEYESSFLHAKVAVVDAQGRRAWATVGSS
                     NLDPLSLLLAREANVVVRDAAFAQQLHARLVRARDAGGRPLDPHEYAARPWRERVLDR
                     VAFGLMRAALWVTGNRY"
     gene            21595..22320
                     /gene="folE"
                     /locus_tag="PK518_04085"
     CDS             21595..22320
                     /gene="folE"
                     /locus_tag="PK518_04085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRM48121.1"
                     /translation="MSSEPVAPSNASTPDEGLPVSVKIRERLAAARRRFHANDNIAEF
                     IRPGELEQLLDEVEAKMQGVLDSMVIDTAGDHNTQNTARRVAKMYLNEVFKGRYVHPP
                     AITEFPNAEHLNELMIVGPITVRSACSHHFCPVIGKIWIGVLPNEHTNVIGLSKYARL
                     VDWVMGRPQIQEEAVVQLADLIMEKTQPDGLAIVMEASHFCMSWRGVREMDSKMLNSV
                     MRGVFLTNSTLRREFLALIPGRN"
     gene            22323..22745
                     /locus_tag="PK518_04090"
     CDS             22323..22745
                     /locus_tag="PK518_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HRM48122.1"
                     /translation="MLVRLLYVSRAVDTSPAAIEAILTQSRQHNPATGITGVLCYGGG
                     IFLQAIEGGRSTVSELYGHIQRDARHKDVELLAFEEICERRFGGWTMGHVNLAKLNHS
                     IVLKYSEKPEFDPYMSSGKVSFALLEELMATASIIGRA"
     gene            22978..23859
                     /locus_tag="PK518_04095"
     CDS             22978..23859
                     /locus_tag="PK518_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM48123.1"
                     /translation="MSLNAFALIILAGLIHAVWNIAAKKAGGDARFAFFTAVLMMLVW
                     APLGWWLGRSAVPLWGAREWAIVALSGVLHVLYYVVLLRGYRKADLTVVYPLARGTGP
                     LVTALVAVTLLGERLSLTAVLGIAGVVAGVFLIAGGPGLLRAGRDEQARRLVRKGMQY
                     GVLTGLFIASYTVVDGYAVKVMLLSPILVDYMGNFVRVLVLAPVALRKPAELAPLWAA
                     QWRFALLVAAVSPVAYVLVLFAMQSAPLSHVAPAREVSMLFAALLGGHLLGEGDRAAR
                     LAGALLIACGVVALALG"
     gene            complement(23867..24475)
                     /locus_tag="PK518_04100"
     CDS             complement(23867..24475)
                     /locus_tag="PK518_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48124.1"
                     /translation="MDFFLLLALGATGAYVLNHQQQRQRIALLARHLHPYQIERLMEQ
                     LTQGYLRAMGEQGDERRQQVLSLLAESETQLVEQFERFVDDFRRVPTALARVSRLPLG
                     LPFATQLLPAATFDMRELLAIHAAGMGRALRNEGNLAARERAFTMTAELLLMQHSCHW
                     FCKSKTVASARMLARHQTPHAQLLASVSPETRRSYLALTGPV"
     gene            24564..25208
                     /locus_tag="PK518_04105"
     CDS             24564..25208
                     /locus_tag="PK518_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRM48125.1"
                     /translation="MPSIETLIAFFGVSVLLALTPGPDNIFVLLQSAQRGWRAGMCVV
                     LGLCLGLVGHTAAVALGLAAVFAASSMAFTVLKVVGAAYLAWLAWGALRAPASVHEAP
                     PGGAAAAGGNGGALRMVGRGVVMNLTNPKVLVFFLAFLPQFADPAQGGMARQIMLLGL
                     VFMLATLLVFGAIACFSGAFGALLQRSARAQRLLNRVAGLVFLGLALRLATAQR"
     gene            complement(25267..>26399)
                     /locus_tag="PK518_04110"
     CDS             complement(25267..>26399)
                     /locus_tag="PK518_04110"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRM48126.1"
                     /translation="PLGTAAVPLVPASADAPPSARLREAGAVLIAKTTMPDYGMLSSG
                     LSSFHALARNPWDLSKGPGGSSAGAGAAAAAGYGPLHVGTDIGGSIRLPASWCGIFGL
                     KPSLGRVPIDPPYTGRAAGPMTRTVQDAALMMQVLSRPDARDSMSLPPQDIDWLRLAP
                     GLERLRGLRLGLLLDAGCGLATDPEVRAAVESAARLFEQAGAIVEPMRPFLTRQMLDG
                     MDHFWRMRSLIDMRQLAPGQLDKVLPFIRQWAESAAAFDGAQVFEAGAQFHATRLATV
                     RACAPFDYVLSPVAPITAFAAELPAPTNDPLQPLEHIAFTVPFNMSEQPAASIHCGHS
                     ATGLPIGLQIAGQRFDDLGVLQIAHAFEQLRGPQRPWPQPPE"
BASE COUNT         4463 a         8494 c         8839 g         4603 t
ORIGIN      
        1 gcagcggcca caggtactgg ttccagccgt agatgaactg gatcacgaac agcgccgcga
       61 tcgaggtgcg cgacagcggc accaggatgt ccttgaagaa gcgcatgggc ccggcgccgt
      121 cgatgcgcgc ggcctccacc agctcgtcgg gcacggtgag gaagaactgg cggaacaaaa
      181 aagtggccgt ggccgaggcg atcaggggca ggctcaggcc ggcgtagctg ttcaaaaggc
      241 ccagatccgc caccaccttg taggtgggca atatgcgtac ctcgacgggc agcatcagcg
      301 tcaaaaagat cgcccagaag caggccatct tgaacgggaa gcggaagtag acgatggcaa
      361 acgccgacaa gagcgagatg gcgatcttgc ccaccgtgat gatgatggcc acgacgaagc
      421 tcacccacat catgcgcacc acgttggtgt tggagcccag cttgcccgag cccagcagcg
      481 cgtcgcggta gttctcccac atgtggctgc ctggcaagag cggcatgggt gattgcacga
      541 tggtctcggc ctggtgcgta ctggcgatga gcgccaggta gagcggaaag gcgacgatgg
      601 ccacgcccag gatgagcacg gcgtgggaca ggaaagtcag ccagggattg cggtcaacca
      661 tgtcagtact gcactttctt ttccacgtag cggaactgga tcaccgtcag caccacgacg
      721 atggccatca gcaccaccga ctgcgcggcc gagccgccca gatccagcgc cttgaagccg
      781 tccagataga ccttgtagac caggatggag gtggactgcc ccggcccgcc ctgggtggcg
      841 gcgtcgatga tgccgaaggt gtcgaaaaag gcgtagacga tgttgatcac cagcaggaag
      901 aaggtggtgg gcgacaacag cggcagctgg atgttccaga agcgccgcca ggggccggcg
      961 ccgtcgatgg aggcggcctc gatcagggcc ttgggtatcg actgcaggcc cgccagaaag
     1021 aacagaaagt tgtacgagat ctgcttccac accgaggcca gcacgatcag cgccatcgcc
     1081 tggttctcgt tcatcaggtg gttccagcca tagcccagct tgcccaggta gtaggtcacc
     1141 acgccgatcg agggcgagaa caggaacacc cacagcacgc cggcaatcac tggcgccacg
     1201 gcgtagggca cgatgagcag ggtcttgtag accatggcaa agcgcacgat gcggtcggcg
     1261 aagatggcca gcgccagcga caccacgatg ccaatgcccg ccaccagcac cgaaaacagc
     1321 gccgtgcgct ggaaggagcc caggtagtca gggttgtccc agagttggcg gaagttctcc
     1381 agccccaccc actcgcggct catgccgaag gcgtcctcca cctggaagga ctgcagcacg
     1441 gcctggcccg cgggcaaaaa gaagaagatg ccgatgatgg ccagctgcgg ggcgatcaac
     1501 acccagggca gccaggcgga gcggaagaga acgcgttttt ccatggccat ggcgcatcac
     1561 ccaggaaaaa aaccagcacc cgcggtggcg gggcgctggc gatagggttg tgaaatactc
     1621 atatttttat agctgcttgc gctttattta taagggctag cggccaattt tcttcaagcc
     1681 ctcccggtag ggggagggcg aagcgctagg cttacttctt gtacgacttc tcaaagcgcg
     1741 ccagcagctc gttgccacgg ctgacgatgg cgtccagcgc ctccttggcg gtcttcttgc
     1801 cggaccagac ctgctccagt tcctcgtcct ctatggtgcg gatctgcaca tagttgccca
     1861 ggcgtatgcc gcgcgagttg tcggtcacct tgcggatcat ctggttcacg gccacgtcgg
     1921 tgccggggtt cttttggtag aagccggact tgtcggtcag ctcgaacgcc gccatggtga
     1981 tcggcaggta gccggtgcgc tggtggctgg cggcctatac cttggtctgc gacaggaatt
     2041 cgaagaactt ggccacgccc ttgtattcag gggccttctt gccggacatg acccacagcg
     2101 aggcgccgcc gatgacggtg ttctgcggcg cgcccttcac gtcggcgtag tagggcatgg
     2161 gggccaggcc gtagttgaac ttggcgttct tggccacgtc gccgtagtag cccgaggagg
     2221 tctggatcat ggcgcattca ccggcggtga acgaggcctg ggcgtccgag cgacggccct
     2281 tgtagacata ttcgccggcc ttggcggcct gcgccaggtt gtcgatgtgg cgcacatgca
     2341 gcggcgagtt gatcttcatg cgggccttgt agccgttggc ggacaggccg ttcctctcgg
     2401 tggcaaattc cacgttgtgc caggcggaga aggattcgag ctgggtccag ccgttccagg
     2461 ccagcgtcat ggggcagctg tggccgctgg ccttgagctt cttggcggcc tcgaacacct
     2521 cgggccaggt ggcgggggcc ttgtcggcgt tcaggccggc cttcttgaag gcgtccttgt
     2581 tgtaataaaa gatggtggtc gagctgttga acggaaagct cagcatctgc ccgtcgggcg
     2641 ccgtgtaata ggcggccacg gccgggatgt agccggccgg gttgaagtcc gcgccggcgt
     2701 ccttcattac cttggccacg ggcacggtgg cgcccttgct ggccatcatg gtggccgtgc
     2761 ccacctcgaa cacctgcagg atgtgcggcg cattgcccga gcggaaggcg gcaattgcgg
     2821 cggtcatgct ctcgtcatag ttgcccttga aggtgggcac caccttgtag tccttctggc
     2881 tggcgttgaa gtcccgggcc aggtcgttga cccactcgtt gttcacggcg gtcatggaat
     2941 gccaccactg gatctcggtc tgggcctgcg cggccaggcc ggtggcgagc agggacgcgg
     3001 ccagggccag ttgcttgaac tgcataaata ctcctcgttg atggacgaca gacatgaagc
     3061 ccgccatggt gtggccgctt catgacaaca cggtgtcgtt gtcgcatgct gaaaatgcgg
     3121 caacaaccgg ggaaacccgc cagccggcgg aacggtcaaa ttgtgacatt ggcgtgatgc
     3181 tttgtgtgcg aatctgcgcg ttttggcgcg gtttatggcg ctatcgcgag cagggatatg
     3241 ccatttcccg gatgtcggca aggatttgtg cgctgcacaa tgaacggcgt tgctgaattt
     3301 tcttcttctt tgacccaccg cgccgcaggc gcccacctgg attgccaagc catgcccaag
     3361 acctccctgg ataaaagcaa gatcaagttt ctgctgctcg aaggcgtgca cgagagtgcc
     3421 gtgcaggtgc tgcgcgccgc cggctacagc cagatcgagc tgctgcccgg cgcgctggag
     3481 ggcgaggagc tgcgcaagaa gatcgccgac gtgcacttca tcggcatacg ctcgcgcacg
     3541 cagctcacgg ccgaggtgct ggcgcaggcg cacaagctgg tggccattgg ctgcttctgc
     3601 ataggcacca accaggtcga tctggacgcg gcgcgcgagc gcggcattgc ggtgttcaac
     3661 gcgccgtatt ccaacacccg atcggtggcc gagctggtgc tggccgaggc catcctgctg
     3721 ctgcgcggcg tacccgagaa gaacgccgtg gcgcaccgcg gcggctggct caagagcgcc
     3781 gacaacgcct tcgaggcgcg cggcaagatc ctgggtatcg tcggctacgg cgccatcggc
     3841 tcgcagctgt cggtgctggc cgagagcctg ggcatgcagg tcatctttca cgacgtggtg
     3901 aacaagctgc cgctgggcaa tgcgcgccag gcggcgggcc ttgccgatct gctcgggcgc
     3961 gccgacatcg tcagcctgca tgtgcccgag ctgccctcca cgcagtggat gatgggcgcg
     4021 gcgcagatcg cggccatgaa gcccggcgcc atcctcatca acgcctcgcg cggcacggtg
     4081 gtcgagatag agccgctggc ccaggcgctc aaggacaaga agctgctggg cgcggcggtg
     4141 gacgtgttcc cggtcgagcc caagagcaac aaggacgagt tccagtcgcc gctgcgcggg
     4201 ctggacaacg tcatcatcac gccccatgtg ggcggctcca cgcaggaggc gcaggccaac
     4261 atcggcctgg aggtggcgga aaagctcgtc cgctacagcg acaacggcac cagcaccaca
     4321 tcggtcaact ttcccgaggt ggcgctgccc gcgcacccgg gcaagcaccg catcctgcac
     4381 atccaccgca accagccggg cgtgctgtcg gccatcaacc aggtgtttgc acagctgcac
     4441 atcaacatcg ccgcgcagta cctgcagacc agcgacaagc tgggctatgt ggtgatcgac
     4501 ctggacgcgg cctcgtccgt gcaggcgctg gaaaagctgg cccaggtgga tggcacgctg
     4561 cgttgccgcg tgctgttctg aggttgcttc tataaatata gctttcagcg cttggtgcat
     4621 aagcgctagc ggctgttttt gcttgtattc aacctgggac atcctgctgc gccgtagtgg
     4681 cacttggcgg cggggcgcgc ccttgggggg cctggcatag aattcccggc cccgaggcat
     4741 gagggcgcgc cgcgcccaac ccaggttgac cccatgaatg caccgatagc gcacgcagcg
     4801 ctgctggcgg ctggcacaga gcccgtgcgc ctgcgcgaga ttccgtacaa ctacaccagt
     4861 ttttccgatc gcgagattgt catccgcctg ctcggtgccg aggcctgggg cgtgctcgac
     4921 cagctgcgcc gcgagcgccg cactggccgc tcggcgcgca tgctctacga ggtgctgggc
     4981 gatatctggg cggtgcagcg caacccctac ctgcaggacg acctgatcgc caaccctcag
     5041 cggcgcaaga tgctggtgga cgccatgcag caccgcatgg gcgagatcga aaaacgccgt
     5101 acgccacagg aggatgccgg gcgcgaccgc cgcgtcggcc tgctggtgca ggcggcgaac
     5161 cgcgccatca ccgagttcga cgccacgctg gtcgaggtga cggccctgcg caagagcgtg
     5221 gcaaagcgcc tgggcaagca cacggcgcgc gacaacatca agttcgacgg cctctcgcgc
     5281 gtgagccatg tgaccgacgc gaccgactgg cgcgtggagt tccccttcgt cgtgctcacg
     5341 cccgacaccg aggcagagat ggccgccctg gtggccgcct gcatagcgct ggagctgacc
     5401 atcatcccgc gcggcggcgg caccggctac accggcggcg ccattccgct gacctggcgc
     5461 agcgccgtca tcaacaccga aaagctcgaa gccatgagcg aggtggagat gcgcaagctc
     5521 cccgggctgg acacggaagt cgccacggtc tggagcgagg ccggcgtggt cacgcagcgc
     5581 gtggccgatg cggccgaacg cggcggctat gtgttcgccg tcgatcccac gtccatcgag
     5641 gcatcgtgca taggcggcaa catcgccatg aacgccggcg gcaaaaaggc cgtgctctgg
     5701 ggcacggcgc tggacaacct ggcgagctgg cgcatggtca cgccggatgc acagtggctc
     5761 gaagtcacgc gcatcaatca caacctgggc aagatccacg acgtggaggt tgccagcttt
     5821 gaattggcct actttgaggc cgacggcaag actgccatcc gcaccgagcg cctggacatc
     5881 ccgggcaaga cctttcgcaa ggagggcctg ggcaaggacg tgacggacaa gttcctcagc
     5941 ggcctgccgg gtgtgcagaa agagggcacg gacggcctga tcaccagcgc gcgctggatc
     6001 gtgcaccgca tgccggcgca cacgcgcacc gtgtgcctgg agttcttcgg ctcggccaag
     6061 ctggccgtgc ccagcatcgt cgagatcaag gacttcatgt ttgccgagca aaagcgcagc
     6121 ggtgtcttgc tggcggggct tgagcacctg gacgaccgct acctgaaggc cgtgggctac
     6181 gcgacgaaat ccaagaaggg cgggggcaag ctgccgaaga tggtgttgat cggcgacatc
     6241 gtcggtgacg accaggacgc cgtggcgcgc gcggccgccg aggtggtgcg catcgccaac
     6301 tcgcgcgacg gcgagggttt cacggccgtg agcagcgagg cgcgcaagaa gttctggctc
     6361 gatcgcaagc gcacggcggc gatctccaag cacaccaacg ccttcaagat caacgaggac
     6421 gtggtcatcc cgctgccgcg catggccgag tacaccgacg gcattgagcg catcaacatc
     6481 gaactcagct tgcgcaacaa gctggcgctg tgcgacgcgc tgcgggactt cctggcccgc
     6541 ggcgacctgc ccttgagcca gcagggcgac gcccagggca ttggcgccga cgtcatgctg
     6601 ggcgaccgcg tggcccaggc gcaagcgctg gtggccgagg tgcgcgcgct ctggcagggc
     6661 tggctcgatg gtgtcgaggc gctgttcccg cagctgcagg accattcctt gcgcgccagc
     6721 tggaagagcc agctgcaaaa gccgctggcc gagatcttca ccggcgccgc cttcgagccc
     6781 atcgtcaaag agaccatcgc catccaccag cgcgtgctca agggccgcgt ctgggtggcg
     6841 ctgcacatgc atgccggcga cggtaatgtg cacaccaaca taccggtcaa cagcgacgac
     6901 tacgagatgc tgcagaccgc gcaccaggcc gtggcgcgca tcatgcagct ggcgcgcagc
     6961 ctggatggcg tgatctctgg cgagcatggc ataggcatca cgaaactgga gttcctcacg
     7021 ccggaggagc tggcgccgtt tgccgagtac aagcgccgcg tcgaccccga agggcgcttc
     7081 aactggggca aattgctacg taatcaggag ctacttgcgc aatacccaca agggcaaaac
     7141 gccgatattg cccaaaaaca ctcgcgcacc tcgctgcgct acgccgacct gaccaacgcc
     7201 tacacgccca gctttggcct gatgggctac gagtcgctga tcatgcagca gagcgacatt
     7261 ggcgccatcg tcgccagcgt caaggactgc ctgcgctgcg gcaagtgcaa gcccgtgtgt
     7321 tcgacgcatg tgccgcgcgc caacctgctc tacagcccgc gcaacaaaat cctggccacc
     7381 tcgctgctgg ccgaggcctt tttgtacgag gagcagacgc gccgtggagt gtccagccac
     7441 cactggcagg agttcgaaga cgtggccgac cactgcacca tctgccacaa atgcttcaac
     7501 ccctgcccgg tgaagatcga ctttggcgac gtcaccatgg ccatgcgcaa cctgctggtg
     7561 aagatggaca aaaagagctg gcgcccgggc aacaagctgg ccatggccat gctcaacgcc
     7621 accagcccga cgaccatcaa cgtgctgcgc gcgggcatgg tgaacgtggg ctttccggca
     7681 cagcgcctgg ccgtcgacgt gatgaaggcg gtggccaagc ctcaggtgac gcgcccgccg
     7741 gccacggtgg gcaccgcgcc gatcaaggag caggtgatcc acttcgtcaa caagaagctg
     7801 cccggcggcc tgccgaccaa gacggcgcgc gcgctgctgg acatccagga caaggactac
     7861 gtccccatca tccgcaaccc gcagaccagc gccgacagcg aggcggtgtt ctacttcccc
     7921 ggttgcgggt cggaaagatt gttcagccag gtcggcctgg cgacgcaggc aatgctctgg
     7981 cacgccggcg tgcaaacggt gctgccaccc ggttacctgt gctgcggcta cccgcagcgc
     8041 ggcaacggcc tgcaggacaa ggccgagaag atgatcaccg acaaccgcgt gctgttccac
     8101 cgcgtggcca ccacgctgaa ctacctggac atcaagaccg tggtcgtgtc gtgtggcacc
     8161 tgctacgacc agctgcaggg ctacgaattc gacaagatct tccccggcag ccgcatcgtg
     8221 gacatccacg aatacctgct ggaaaagggc atcaccttgc ctgcaggcca gggcggctac
     8281 ctgtaccacg agccctgcca caacccgatg aagcagggcg attccatggc caccgtgcgt
     8341 gcgctggtgg gcgacaaggt cttgaagagc gagcgctgct gcggcgaatc gggcacgctg
     8401 ggcgtctcgc gcccggatat ttccacccag gtgcgcttca gaaagaccga ggaaatccgc
     8461 aagggcgagg cccagctgcg cgccagcggc gccgtcggtg cgcaggacaa cgtgaaaatt
     8521 ttgaccagct gccccagctg cctgcagggc atctcgcgct acgagaacga tttgtccacg
     8581 ggcctgctgg aagccgacta catcgtcatc gagatggcca aacagatcct gggcgaaaac
     8641 tggctgcccg agtacgtggc gcgggccaac gccggcggca ttgagcgggt tcttgtatga
     8701 gttgcgtgct gtgcgacagg gacggcggcc agctcgtctg gcgcggcgac aagctgcgcg
     8761 tgatccgcgc cgacgaggcc ggctttccgg ccttctaccg tgtgatctgg aacacgcatg
     8821 tggccgagtt ttccgacctg agcgcggccg agcgcgcgca ctgcatgcag gccgtggccc
     8881 tggtcgagca gaccctgcgc gagcaactgc agcccaccaa gatcaacctg gcggcgctgg
     8941 gcaacgtcgt gccgcacctg cactggcatg tgatcgcgcg ctttgactgg gacagccact
     9001 ttccggcccc ggtgtgggcc ggcgcccagc gcgcgcgcga tgcggcgcgc gaggatgagc
     9061 tgcgcctgcg ccaggtgacg ctggaggcgg caatgcgtgc gcggcttggc gcctggaccg
     9121 gcacgcgctc cgacgattga tgcggtgaaa tttcagaggt aacccatggc cggcctgacc
     9181 cccagcaccc ccactcccga atccatcacc ctgcacgaaa aatcgcgtgc actggagctg
     9241 gccttctccg atggcgcgcg gtttcgcatt ccgctggagc tgctgcgcgt gtgctcgccc
     9301 tccgccgagg tcatgggcca tggcccgggc caggaggtgc tgcagaccgg caagcgcgac
     9361 gtgacgctgg tggacttgca gcccgtgggc aactacgcca tacagccgag cttctccgac
     9421 gggcacaaca gcggcatcta cacctgggcg tatctgtatg agctggggcg cgatcaggat
     9481 gcgctttggc agcgctacct gcagcgcctg cgggacgcgg gactggacag ggatgcgccg
     9541 atggcgccaa aggggcagaa atcgggttgc tccagccatt gatgctatct tcgtcatagc
     9601 tgctggcgct ttacctgtaa gcgcggtaag cattttcttt tttgcgacac atgccctggg
     9661 cggacgcggg gttgtcgctg tacgccgggc gcgcgcgccc ctaggatgca tcgctatatg
     9721 agtaccacgc atttcggatt tgaaacggtt gacgagaaag acaaggcgcg ccatgtgcgc
     9781 ggcgtgttcg actcggtcgc gcccaggtac gacctgatga acgacctgat gtccgccggc
     9841 ctgcaccgcg cctggaaggc ctacacggtg atggtcgcca acctgcgcga gggcgatcag
     9901 gtgctggaca tcgcgggcgg cacgggcgac ctgtcgctgg cgttttccag gaaggtcggc
     9961 agcacgggca gcgtggtgca caccgacatc aacgaggcca tgctgaacgt ggggcgcgac
    10021 cgcctcatca acctcggcgt ggtgctgccc acgctggtct gcgacgccga ggccctgccg
    10081 ttcccggaca accatttcga tctggtcagc gtggcctttg gcctgcgcaa catgacgcac
    10141 aaggatgagg cgctcaagga aatgtgccgc gtgctgaaac cccgcgggcg cctgctggtg
    10201 ctggagtttt ccaaggtggc ccagcctttg gccaaggcct acgactggta ttccttccag
    10261 gtgctgccgc gcctgggcaa gctggtggcg ggcgatgact ccagctaccg ctatctggcc
    10321 gagtcgatcc gcatgcaccc gggccaggat gagctcaagg ccctgatgca acaaagtggc
    10381 tttggccatg tggattatca caacatgacc gggggtattg ccgcgttgca tgtcggaatt
    10441 aaatgctgat gcacaatcga tacctgataa ctatcaacac tttggagtaa cgccatgaaa
    10501 ttgtggtccg tcatcctggt cgccatgctg tcggtcgtgc acctggatgc cgacgcgcgc
    10561 cgcatgggcg gtggcaagtc catcggcaag cagtccagca atgtgacgca gcgcgaggcc
    10621 gcgcctcccg ccgcgcctgc cgcccccatg cagcaaaacg ccgccaatag cgcggcaccc
    10681 aagccggcgg ctgcgcccaa cgctgcggcc ggcgcgccca agaagccctg gggcgccatg
    10741 ctgggcggcc tggccgcggg cttgggcctg gcctggctcg ccaactcgct gggcctgggg
    10801 gcggcctttg gcaacttcct gctgatcgcc ctgctggcgc tggtggtcat ggttgtgatc
    10861 ggcatggtga tgcgtgcgcg caagcccgca gccagccagg ccgccagctc gccctttgcc
    10921 ttccagggcg ctggcgcacc agcacctgtc gacgtgcaga tgccgcgcca gtacaacccc
    10981 gagaaggtcg gcaacgacgc ctcggcgcgt ccctgggagc agttctcggc cagcccgcag
    11041 cagggcggcg gcagcatgat cggttcggcc ctggcgggtt cgcagaactg gggcgtgccg
    11101 gccgactttg atgccgaggg tttcctggct gcggccaagc gcaacttcac cacgctgcag
    11161 gccgcctggg acaggtcgga catcgccacg ctgcgctcca tgatgaccga cggcatgctc
    11221 gacgagatcc gtgcccagct cgccgagcgc gaggcacagc gcgctggcga gcacccgaac
    11281 cacaccgaag tggtgatcct ggaggcgcag ctgctgggca tcgaggatct gggcgatgcc
    11341 tacatggcca gcgtggagtt ctccggcatg atccgcgagg agccctcggc tggccccagc
    11401 ccgtttcgcg aggtctggaa catgaccaag cccaagagcg gcaccagcgg ctggctggtg
    11461 gcgggcctgc aggccttgca ataagcagcc ggcgctggcg gcaatcaagc cgcggcgccg
    11521 aatgatcaga taatcggggg ctatggcaac acagtcccct ttttcgtttc tggacgggct
    11581 actccagcgc gtcctggccg gcccgcagcc gcccgcctgg ctggtgcatg agctgcagca
    11641 ccgcctggtg ctgtttttga accatgtgct gatgcaggaa agagaggcca tggagcgcct
    11701 gacgcgccag cgcggccggg tggcgctggt gcaatggcga gtcttcacca tggcgctgca
    11761 ggtcaccccg gcggggctgc tggatctggc gccgctgggc gtggtgcctg atctgcgcgt
    11821 ggaggtgtcc gacagctccc cgctgtcgct ggccaagggt gcgctgcgtg gcgacaagcc
    11881 cgccattcgc atcgacggtg acgtgcaact ggcagccgac atcaactggc tggtcgatca
    11941 tgtgcgctgg gacgtggagg aagacctggc gcgcgtcatt ggcgacgcgc cggcgcgcgc
    12001 gctcgccgcc gtcgcgcaac gcgctgccgg ggcgctgcgc ggcttcgtcg gcgctcgcat
    12061 gggccagggc gccggtgctt cggatcgcat gcgtctatga gtcgtttttt tcgcggcatc
    12121 agcatcgtct gggtggtgtt gcgctatggg ctcgatgagc tggtgctgtc cagcttccag
    12181 cacccgtggc tgcgcgcgct cacgcgcatc atcaccatag gccgcagctt ggacgccccg
    12241 cgcggccagc gcctgcgcga ggcgctggag agcctggggc cgatcttcgt caagttcggc
    12301 caggtgctgt ccacccggcg cgacctgatg ccccccgaca tcgccgacga actggcgctg
    12361 ctgcaggatc gcgtgccgcc attcgatccc gacattgcca ttgccaccat agagcgctcg
    12421 tttcgccgcc ccctgggcga aatctttgtg tccttcgagc gcgagcccgt ggccagcgcc
    12481 tccatcgccc aggtgcattt cgcggtgatc cgcgaccgcg atgggcgcga gcgcgacgtg
    12541 gccgtgaagg tgctgcgccc caacatgctg ccggtgatcg acaaggatct ggcgctgatg
    12601 cgcatgatgg ccggctgggt ggagagcctg tcggccgacg gcaagcgcct caagccccgc
    12661 gaggtggtgg ccgagttcga caactacctg cacgacgagc tggacctggt gcgcgaggcg
    12721 gccaatgccg cccagctgcg gcgcaacatg gaggggctgg acctggtgct catccccgag
    12781 attttttggg atttctgcca tgccgacgtg gccgtgatgg agcgcatgaa tggcgtgccc
    12841 atcagccagg ttgatcgcct gcgcgccgcc ggtgtcgata tcccaaaact ggcacgcgac
    12901 ggcgtgacca tcttcttcac ccaggtgttc cgcgacggct tcttccatgc cgacatgcac
    12961 ccgggcaaca tccaggtcag cctggatggc agcagctttg gccgctatat ctcgctcgat
    13021 ttcggcatcg tcggcacgct caccgagttc gacaaggaat acctggcgca gaacttcacg
    13081 gccttcttca ggcgcgatta caagcgcgtg gccgagctgc acatcgaaag cggctgggtg
    13141 cctgcgagca cgcgcgtgaa cgagctggag gcggcgatac gcaccgtgtg cgaaccgtat
    13201 ttcgaccgcc cgctgaagga gatctcgctg ggcatggtgc tattgcgcct gttccagacc
    13261 tcgcggcgct tccaggtgga gatccagccg cagctggtgc tgctgcagaa aaccctgctc
    13321 aacatcgagg gcctgggccg ccagctcgac cccgacctgg acttgtggag cacggccaag
    13381 cccttcctgg agaagtggat gctggaccag ctgggcccgc agcgcctgtg gcgcgagctc
    13441 aaggccgagg cgccgcatta cgccaagatc ctgcccgacc tgccgcgtct gctgcacgac
    13501 tatctgcgcc agcgcccgca tgacagccgc gccgacctgc aggcgctgct ggccgcgcag
    13561 cagcgcacca accggctgct gcaaggcatc atctacggcg ccctgggctt tgtgctcggc
    13621 ctgctggcga tgcagctggt ggtgcgcgta caccttttct gaaccactcc ccatccattt
    13681 tttcctccag gagcttcaac gtgttgatct tcatggtgat tgcctacctg ttcgtcacca
    13741 tcggcatcgg cctgtgggcc gcacagcggg tgaagaacac ggccgacttc gcgattgccg
    13801 ggcgcaacct gcccatgtac atgatcatca ccaccacgtt tgccacgtgg ttcggttcgg
    13861 agatcgtgct gggcgtgccc gccaagttca tccagggcgg gttgaatgcc gtggtcgagg
    13921 atccgttcgg cgccggcatg tgcctgatcc tggtgggcct gttcttcgcg gccaagctct
    13981 accgcatgac gctgctgacc atcagcgact attaccgcga gcgctatggc cgggtggtgg
    14041 agatcatctg ctcgctgatc atcatgctca gctacctggg ctgggtgtcg gcccaggtca
    14101 cggcgctggg gctggtgttc aacctgctct cgggtggctc gctcagcatt ccctggggca
    14161 tgaccatagg cgtgctgtcc attctggtct ataccctctg gggcggcatg tggtcggtgg
    14221 ccgtcacgga cttcatccag atgatcatcc tggtgctggg cctggtgatc ctgtcctggt
    14281 ttgctgccga catggccggc ggcgccggca aggtggtgga tctgatcacc agccgcgaca
    14341 tgctgcgttt ctggcccgag ccgacctggc atgagatgct gtttttcttc ggtgccgcca
    14401 tcaccatgat gctgggctcg attccgcagc aggacgtgtt tcagcgcgtg atgtcggcca
    14461 acaccgaaaa ggccgccacg catggcacgg tgatcggtgg ctcggcctac atcctgttcg
    14521 ccttcgtgcc catgttcctg gtggccagcg ccctgctcat catgcccgag caggccgccg
    14581 agctgctgca ggaggatccg caaaaggtgc tgcccaccct ggtcatgacg cgcatgccgc
    14641 tggtcatgca ggtactgttc ttcggcgcgc tgctctcggc catcaagtcc tgcgcctcgg
    14701 ccacgttgct ggcccctagc gtcaccttta ccgagaacat ctggcgccag ttccgccccg
    14761 agcacatcag cgaccgcgaa aagctgctca ccatgcgcat cagcgtgctg gtctttgccg
    14821 cgggcgtgct ggtctactcc atcaagatgg agggcacgcc gatttatgag ctggtgtcca
    14881 gcgcctacca ggtgccgctg gtgggggcct ttgtcccgct ggtgttcggc ctgtactgga
    14941 agcgtgccaa cacccagggc gcggtctgcg ccgtggtgct gggcattggc gtgtgggtgg
    15001 tgttcatgct cacgcccgcg ctgcacgagg cctttccgca gcagctggcc ggcctgttgt
    15061 cggcggtgtt cggcatggtg gccggctcgc tggcgccgca gatggtgcgc aatacgcgcg
    15121 cgccgcacca ccgggtggtg ggggtggagt agggcgcgcc ggctgtctgc tgcgcgccta
    15181 cctataatca aaagctttgc acatttttcg cgctacttcg caatgcctat ttacgcctac
    15241 caatgcggca cctgtggcca tgccaaggac gtgctgcaaa agatgtccga tgcgcccctg
    15301 accgactgcc ccgcctgcgg cgcgcagacc ttcaccaagc agctgacggc cgccggcttt
    15361 cagctcaagg gctcgggctg gtatgtgacc gacttccgcg gcggcgccgg tggcactgcg
    15421 gcggccgctc cagccgctcc ggacgctcct aaaaaagaag ctgttagcgc tgcaccagca
    15481 agcgctccag ccagttctga cgcataaggc tgaacatctt catggctgca ctgcgcaaat
    15541 ggctgttcac gggcctgctg gtcatcgtgc ccggcgtcat caccgcctgg gtgctgacct
    15601 ggatagtgag cacgctcgat cagacgctga ccatcctgcc cggggcctgg cacccggatc
    15661 ggttgctggg ctttcacatt cccggctttg gcgtgctgct cacgctggcc atcctgctca
    15721 tcgtgggcgc ctttgccagc aacttcgccg ggcgcaagct ggtgtcgctg ggcgacagtc
    15781 tggtgacgcg cataccggtg gtgcgctcca tctactccag cgtcaagcag gtgtcggaca
    15841 cgctgttttc cgagagcggc aacgcctttc gcaccgccgt gctggtgcag tggccgcgcg
    15901 atggcgtgtg gacggtggcc ttcatcaccg gccagcccag cggcgaggtg gcaggctatc
    15961 tgcgcgatga attcgtcagt gtctatgtgc ccaccacgcc caacccgacc agcggctact
    16021 tcgtgctcat gcgcaagagc gactgcgtgg agctggacat gagcattgac gcggcgctga
    16081 aatacatcgt ctccatgggt gtggtggccc cgcccgatcc ggtagcgctg gaagctgcga
    16141 agtaagacaa accaaggttg cgcgctcccg ggtgcccgtt catcggcggc gcgcagaaag
    16201 acaacccaac acaagcgccc cacgagggcg caggaagctg aacccatcat ggccatgcgc
    16261 tctgaatact gcggtcttgt gaccgaagcc ctcctgggcc aaaccgtcag cctgtgcggc
    16321 tgggtgaacc gccgccgcga ccatggcggc gtgatcttca tcgacctgcg cgaccgcgaa
    16381 ggctatgtac aggtggtctg cgacccggat cgcgccgaga tgttcaaggt ggccgaggac
    16441 atccgcggcg agttctgcgt acgcgtggtg ggcctggtgc gcgcgcgtcc cgccggcacc
    16501 accaacgaca agctcaaaag cggccagatc gaggtgctgt gccacgagct gacggtgctc
    16561 aacgcctctg tcacgccgcc cttccagatg gacgacgaca acctgtcgga gaccacgcgc
    16621 ctgacgcacc gcgtgatgga cctgcgccgc ccggccatgc agaagaatct gatgctgcgc
    16681 tacaaaacgg ccatccaggt gcgcaactac ctcgacaagc tgggcttcat cgacatcgaa
    16741 actcccatgc tgggcaagag cacgcccgag ggcgcgcgcg actacctggt cccgagccgc
    16801 gtgcacgacg gccagttctt cgccctgccg cagtcgcccc agctgtacaa gcagatgctg
    16861 atggtggccg gctacgaccg ctactaccag atcaccaagt gcttccgcga cgaagacctg
    16921 cgcgccgacc gccagcccga gttcacgcag atcgactgtg agacttcctt cctgggcgaa
    16981 gaagagatcc gcgccatctt ccagcagatg atcaaggaag tgttccagac gcagctgaat
    17041 gtcgatctgg gcgagttccc catcatggcc taccaggagg ccatggcacg ttttggctcc
    17101 gacaagcccg acctgcgcgt caagcttgag ttcaccgaac tcaccgacgt gatgagggac
    17161 gtggacttca aggtcttctc cggcgctgcc aacatggcgg gcggccgcgt ggtggcgctg
    17221 cgcgtgcccc atggttccgt cgagaacggc ggcatcagcc gcggcgagat cgaccagtac
    17281 accgagttcg tgaagatcta cggcgccaag ggcctggcct atatccgcgt caatgagctg
    17341 gccaaggggc gcgacggcct gcagtcgccc atcgtcaaga acatccacga cgccgccctg
    17401 gccgaggtgc tcaagcgtag cggcgcgcag gacggcgacc tgatcttctt cggcgccgac
    17461 aaggcaaaga tcgtcaatga cgccatcggc gcgctgcgcc tgaaaattgg ccacagcgaa
    17521 ttcggcaaga agcacggcct gttcgaggat cgatgggcac cgctgtgggt ggtggacttc
    17581 cccatgttcg agcatgacga ggaagacaac cgctgggtgg ccgtgcacca ccccttcacc
    17641 gcccccaagg acggtcacga agacctgatg gacaccgacc cggccaagtg catcgccaag
    17701 gcttacgaca tggtgctgaa cggctgggag ctgggcggcg gctcggtgcg tatccaccgc
    17761 gccgaggtgc aggccaaggt attcgccgcc ctgcatatca cccccgagga tcagcgcgcc
    17821 aagttcggct acctgctcga cgccctgcag tacggcgcgc ccccgcatgg cggcctggcc
    17881 tttggcctgg atcgtttgat cacgctgatg accggcgccg aatccatccg cgacgtgatc
    17941 gccttcccca agacccagcg tgcgcaggat ctgctgaccc aggcgccgtc gccggtggat
    18001 gagaagcagc tgcgcgagct gcatatccgc ctgcgtaacc cggcggtcgt aggctgagca
    18061 ttgccgtcgg ccgtgttgcc cgccggatca aaagggcgcc tcaaacgggc gccctttttg
    18121 catcatggcg acccgccgag cagggcctcc ttgagtttcc agtcggcggg ggcagggcca
    18181 aaccagatcg acatcagggc gcggaaaaaa gccgcatccc tggaaggttc gccttgctgc
    18241 ctgcctttga cccagatgct caggcccttt gcaggatccc actcgacgga acacacgtcg
    18301 ccgggggaga acgattgatg cagtgcaaaa tattcactga tcttcaccat acccgggatg
    18361 atctggggaa attcctgccg gctgacgttg tcttcaatcc cttgcgtcaa catcttgcca
    18421 aaatccgacg acttgacctc ccgcataaac gtcatgctga ggcgcttggg gcctggggca
    18481 gcaatcagct catcgagtga tttgattggc ttgctggcgt aaaggctgcc gacataaacc
    18541 ttgaaagggc ctttgaatcg gattcccgcg ccattcagcc ggagctccga cccggcgacg
    18601 atggccgtct cctgcacctg cacgccggca atcgcgacgg ccgaccaggc cggggctccc
    18661 cagcacaggg agccaagtag acagaggatt tgcaatgttt tgcgcatgac tgactctgaa
    18721 aagaacgacc gttcacttat gaaacaaatt gtgccgaatt ctatggcaag tactttcagg
    18781 gtttgtactg ggcgatttgc cgctggaggc gtgacagccc cactggttgc gtggccgcta
    18841 ctccattggt gctccgtcct tggcgtggca ggcgagtcgg tgcagaggct gcggcatccg
    18901 gctgcatttg tgctggcgcg cctgccgaga gtgccctccg gtgcgccgac ccatggtttg
    18961 gcaagactcc tgcaggccac ggctgttatc cactgcgggt gctgaggcga acgattcaga
    19021 ttcgccacca caaccatggc atacaaaatc cccgagtcgg tgttggtggt catccacacc
    19081 cccgacctgc aggtgctgct gatccgccgc gccgatgcgg ccgaggactt ctggcagtcc
    19141 gtcaccggta gcaaggacag cctgggcgaa gccttggccg ctacggccgc gcgcgaggtg
    19201 cgggaggaga caggcattga cgccggcgcc cccggctgcc ggctgcgcga ctgggggctg
    19261 gagaatgttt acgccattta cccgcagtgg cagcaccgct acgcccctgg cgtgtggcgg
    19321 aacaccgagc atgtctttgg cctgcaggta ccgcagggca caccggtggt cttgaatccg
    19381 cgcgaacata cggcatatgc ttggctggat tggcgcgcgg cggcggatcg ctgtttctcg
    19441 ccctcgaatg ccgaggccat actctggctg ccccgatttg ctgcacgatg actgcttttg
    19501 ccatttcaga actgcctgcc gagtattccg gcatcctgcg cgtggccacc tacaacatcc
    19561 acaagggtgt gcagggcctg ggccctgcgc gccggctgga gatccacaac ctgggcctgg
    19621 cggtggagca gctcgatgcc gacatcgtct gcctgcagga ggtgcgccgg ctcaaccaca
    19681 aagaggcggc gtactttcgg cgctggccgc gcgtgccgca ggccgagtac ctggcgcccg
    19741 aagggtatga cgcggtctat cgcaccaatg cctggacacg ccatggcgag catggcaacg
    19801 cgctgctgtc gcgctggccg gtgattggcc atcagcacga agatatttcc gaccaccgct
    19861 tcgagcagcg cgggctgttg catgtcgagg tggaggtgca gggccggcgc gtgcatgcca
    19921 tcgtcgtgca cctggggctg attccgggca gccgcgtgcg ccaggtggcc cagctgcagc
    19981 gcttcatcga gcgcgaagtg cccgccggcg ccccgctggt cgtggccggc gacttcaacg
    20041 actggggcct gcagatcaag cgcatgctgg cgggttttgg cctgcacgag tacgacgatg
    20101 cggcccgcac cttcacctac ccggcgcgcc tgcccatcgt gcagctcgat catgtctatg
    20161 tgcgtggcct cacgccgctg ggcctgcagg taccgcgcgg acgcatctgg tggcgcatgt
    20221 cggaccacct gccgctgatc gccgaattcc ggctctgacc gcaccctatg gtcaccccga
    20281 cgcgcccgac caagccagcc acagaccacc agatccagct gctcgaaggg gcgcgggagt
    20341 tctttccggc cctgatcacc gagatggatg cggccctgtc cgacatccag ttcgagacct
    20401 atatctttga ttgcaccggc acgggcgccg acgtggccga ggcgctgatg cgcgccgccg
    20461 cgcgtggcgt gcgcgtgcac ctggtggtcg atggcgtggg cacgggccgt ctgcccgagc
    20521 cctggcgcac gcgattgcgc tcggccggcg tgcgcatcga ggtgtattca ccgctggggc
    20581 cgctgggcct gctgctgccc aggcgctggc ggcgcctgca ccgcaagctg tgcgtgatcg
    20641 acggctgcgt gctgttctgc ggtggcatca atgtgctgga cgacttttac gaccccaacc
    20701 acgggccgtt ggccgcgccg cgctttgact ttgccgtgcg cgccgtgggc agcctggtgc
    20761 agcatgccag cgacaccatg gagcagttgt ggtggcgcat gcgcgctgtg agtgatgtgc
    20821 gccaccgccg cctgtcgctg gcgctggccg acatgcgagc ggccagcgcg gcgcgcctgg
    20881 ccatcaagcg cgcacaaagc cagcgccgcg gcatgcgtgc cgccctgctg ttgcgcgaca
    20941 acgtgttgca ccgcaccagc atagagcgcg cctacctgcg cgccatcgcc caggcgcggc
    21001 gcgaggtgat catcgccaac gcctacttca tgcccggcgg caagctgcgc cgggccctga
    21061 tattggccgc gcgccgtggc gtgcgcgtgc agctgctgct gcaggggcgc tacgaatatt
    21121 tcatgcaata ccacgccgcg cgccccgtct acggcgcgct gctggccgcg ggcgtggaga
    21181 tctacgaata cgagtccagc ttcctgcacg ccaaggtggc cgtggtggac gcgcagggcc
    21241 ggcgcgcctg ggccacggtg ggttcgagca acctggatcc gctgtcgctg ctgctggcgc
    21301 gtgaggccaa cgtggtggtg cgcgacgcgg cctttgccca gcagctgcat gcgcgcctgg
    21361 tgcgcgcgcg cgacgcgggc gggcggccgc ttgatccgca cgagtacgcg gcccgccctt
    21421 ggcgcgagcg ggtgctcgac cgcgtcgcct tcggcctgat gcgcgcggcg ctgtgggtta
    21481 cgggcaatcg ctattgagct attgtttaca tagctgccag cgcttgttgg ataagcgtta
    21541 ggggcataaa tcaagcataa aactgtgatc agcattcgct gttaccatgc cgccatgtct
    21601 tcagaacctg tagcacccag caacgccagc acaccggacg agggcctgcc cgtttccgtc
    21661 aagatccgcg agcgcctggc cgcggcgcgc cggcgctttc atgccaacga caatatcgcc
    21721 gagttcataa ggccgggcga gctcgaacag ctgctcgacg aggtcgaggc caagatgcag
    21781 ggcgtgctcg acagcatggt catagacacg gccggtgacc acaacacgca gaacaccgcg
    21841 cgccgcgtcg ccaagatgta tttgaacgag gtcttcaagg gccgctacgt gcatccgccg
    21901 gcgatcaccg agtttccaaa cgccgagcat ttgaacgagc tgatgatcgt cggcccgatc
    21961 accgtgcgca gcgcctgcag ccaccacttc tgccccgtga tcggcaagat ctggatcggc
    22021 gtgctgccca acgagcacac caacgtcatc ggcctgtcca agtacgcgcg cctggtggac
    22081 tgggtcatgg gccggccgca gatccaggag gaggccgtgg tgcaactggc cgacctgatc
    22141 atggagaaga cccagcccga cggcctggcc atcgtcatgg aggccagcca cttctgcatg
    22201 tcctggcgcg gcgtgcgcga gatggacagc aagatgctca actcggtgat gcgcggcgtc
    22261 ttcctcacca actccacgct gcgccgcgaa tttctggcgc tgatacccgg aaggaactga
    22321 ccatgctggt tcgcctgctc tacgtcagcc gcgcggtgga cacctcgccc gcggccatcg
    22381 aggccatcct cacgcaatcg cgccaacaca acccggccac cggcattacc ggcgtgctgt
    22441 gctatggcgg cggcatcttc ctgcaggcca tagagggcgg gcgctccacg gtgagcgagc
    22501 tctacggcca tatccagcgc gatgcgcgcc acaaggacgt ggagctgctg gccttcgagg
    22561 aaatctgcga gcgccgcttc ggcggctgga ccatgggcca tgtgaacctg gccaagctca
    22621 accattccat cgtgctcaag tactccgaga agcccgagtt cgacccctat atgtcctcgg
    22681 gcaaggtgtc gttcgcgctg ctcgaggagc tgatggccac ggccagcatc atcggccggg
    22741 cttgaggtgt gcaaccccct gagcggcttg cgccgcctcc cccttctctc tacgcgctgc
    22801 gcgctacggg aagggggacg ccgccagcgc ggcggggcgg cccttgcgcg gcggccctgg
    22861 ctcgggttgc gccagtttca tgggccgcgg gcagcgcacg gcatcatcta taactgagac
    22921 aaatcacata ctgaacgccg cagccggccg caaggcgctg cggtttcttg cgtttgcatg
    22981 tcgctcaacg cctttgccct gatcatcctc gccggactga tccatgccgt gtggaacatc
    23041 gccgccaaga aggccggtgg cgatgcgcgc tttgccttct ttactgccgt gctgatgatg
    23101 ctggtctggg cgccactggg ctggtggctg gggcgctcgg ccgtgccgct gtggggcgcg
    23161 cgcgaatggg ccatcgtcgc cctgagcggc gtgctgcatg tgctgtatta cgtggtgctg
    23221 ttgcgcggct atcgcaaggc cgatctgacg gtggtctacc cgctggcgcg cggcaccggg
    23281 ccgctggtga cggcgctggt ggccgtcacg ctgctgggcg agcggctgtc gctgaccgcc
    23341 gtgctgggca tagccggtgt ggtggccggg gtgttcctga ttgccggcgg gcccggcctg
    23401 ctgcgcgccg ggcgggacga gcaggcgcgc cggcttgtca ggaagggcat gcagtacggc
    23461 gtgctcaccg gcctgttcat cgccagctac acggtggtcg atggctatgc ggtgaaggtc
    23521 atgctgctgt ctcccattct ggtggactac atgggtaact tcgtgcgcgt gctggtgctg
    23581 gcgcccgtgg cgctgcgcaa acccgccgag ctggcgccgc tgtgggccgc gcagtggcgc
    23641 tttgcgctgc tggtggccgc agttagcccc gtggcttatg tgctggtgct gtttgccatg
    23701 cagagcgcgc ccctgtccca tgtggcgccg gcgcgcgagg tctccatgtt gttcgcggcc
    23761 ctgctgggcg gccatctgct gggcgagggc gaccgcgccg cgcgcctggc aggggcgctg
    23821 ctgattgcct gcggcgtggt ggcgctggcg ctgggttgac ccggggttac accggcccgg
    23881 tcaaggccag atagctgcgc cttgtctcgg gcgagaccga ggccagcagc tgggcgtgtg
    23941 gcgtctggtg gcgcgccagc atgcgcgccg aggcaacggt cttggacttg cagaaccagt
    24001 ggcagctgtg ctgcatcagc agcagctcgg ccgtcatcgt gaaggcgcgc tcgcgcgctg
    24061 ccagattgcc ctcgttgcgc agggcgcggc ccatgccggc ggcgtggatg gccagcagct
    24121 cgcgcatgtc gaacgtggcg gcgggcaaca gctgggtggc aaagggcagg cccagcggca
    24181 ggcggctgac gcgcgccagc gccgtgggca cgcggcgaaa gtcgtccacg aagcgctcga
    24241 actgctcgac cagctgcgtc tcgctctcgg ccagcaggct cagcacctgc tggcggcgct
    24301 cgtcgccttg ctcgcccatg gcgcgcagat agccctgggt gagctgctcc atcaggcgct
    24361 cgatctggta ggggtgcagg tgccgggcga gcagggcgat gcgctggcgc tgctgctggt
    24421 ggttgagcac ataggcgccg gtggcgccca gggccagcag aagaaagaaa tccatgggca
    24481 aactgaagtg aaagcgggcg aaagggggtg attgtcgccc gtccggtgtt ggctgccgtg
    24541 gcaggcgcag cacaatggca cccatgcctt ccatcgaaac cctgatcgcc ttctttggcg
    24601 tgtccgtgct gctggccctc accccggggc cggacaacat cttcgtgctg ctgcagtcgg
    24661 cgcaacgcgg ctggcgtgcc ggcatgtgtg tggtgttggg cctgtgcctg ggcctggtgg
    24721 ggcacacggc ggccgtggcc ctggggctgg cggcggtgtt tgccgcctcc agcatggcgt
    24781 tcacggtgct caaggtcgtg ggcgcggcct acctggcctg gctggcctgg ggcgcgctgc
    24841 gggcgccggc cagcgtgcac gaggcgccac ccggcggcgc tgcggcggcc ggcggcaacg
    24901 gcggcgccct gcgcatggtg gggcgcggcg tggtgatgaa cctgacgaat cccaaggtgc
    24961 tggtgttctt cctggccttt ctgccgcagt ttgccgaccc ggcgcaaggc ggcatggcgc
    25021 gccagatcat gctgctgggc ctggtgttca tgctggcgac gctgctggtg tttggcgcca
    25081 ttgcctgctt ctcgggggcg tttggcgcgc tgctgcagcg ctcggcaagg gcgcagcggc
    25141 tgctcaaccg tgtggccggc ctggtgtttc tgggcctggc gctgcggctg gccacggccc
    25201 agcgttgatt cctgatttga tagctgtcag cgcttgatgg ataagcgctg tagcctgttt
    25261 taatgctcat tctggcggct gcggccaggg ccgctgcggc ccgcgcagtt gctcgaaggc
    25321 gtgggcgatc tgcagcacgc ccaggtcgtc aaagcgctgt ccggcgatct gcaggcctat
    25381 gggcaggccg gtggcgctgt ggccgcagtg gatggaggcg gccggctgct ccgacatgtt
    25441 gaagggcacg gtgaaggcga tgtgctccag cggctgcagc gggtcgttgg tgggcgcggg
    25501 cagctcggcg gcaaaggcgg tgatgggtgc cacgggcgag agcacatagt cgaacggcgc
    25561 gcaggcgcgc accgtggcca ggcgcgtggc gtggaactgc gcgccggcct cgaacacctg
    25621 cgcgccatcg aaggcagcgg cgctctcggc ccactggcgg atgaagggca gcaccttgtc
    25681 cagctgcccg ggcgcgagct ggcgcatatc gatgagcgag cgcatgcgcc agaaatggtc
    25741 catgccgtcg agcatctggc gcgtgagaaa ggggcgcatg ggctcgacga tggcgccggc
    25801 ctgctcgaac aggcgcgcgg ccgattcaac ggcggcgcgt acctcggggt cggtggccag
    25861 gccgcagccg gcgtccagca gcaggcccag gcgcaggccg cgcaggcgtt ccaggccggg
    25921 cgccagccgc agccaatcga tgtcctgcgg tggcaggctc atgctgtcgc gcgcgtcagg
    25981 cctgctcagc acctgcatca tcagcgccgc gtcctgcacg gtgcgcgtca tcgggccggc
    26041 ggcgcggccg gtgtagggtg ggtctatggg gacgcggccc aggctgggtt tcaggccaaa
    26101 gatgccgcac cagctggccg gcaggcgaat ggagccgcca atgtcggtgc ccacatgcag
    26161 cgggccgtag cccgcggcgg cggcggcgcc cgcgccggcg ctcgatccgc ctgggccctt
    26221 gctcaaatcc caggggttgc gcgccagcgc gtgaaagctc gacagtcccg aggacagcat
    26281 gccgtagtcg ggcatggtgg tcttggcgat cagcacggcc ccggcttcgc gcagccgcgc
    26341 ggagggtggg gcgtcggcgc tggccggcac caggggcacg gcggccgtgc ccagcggcg
//
LOCUS       DAOMTQ010000024        26077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_190_length_26077_cov_26.675544, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000024 DAOMTQ010000000
VERSION     DAOMTQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 26077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26077
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190_length_26077_cov_26.675544"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK518_04115"
     CDS             complement(<1..566)
                     /locus_tag="PK518_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRM48127.1"
                     /translation="MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKV
                     SPQPLTPAHTLTLARSIMSDRQIADFERTKECNFAISPAGIGRFRANAFIQQGRVGMV
                     LRTIPLTLPTIDGLGVPQVLKEIALAKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
                     HIITVEDPIEFVHPHKNCVVTQREVGLDT"
     gene            complement(591..1220)
                     /locus_tag="PK518_04120"
     CDS             complement(591..1220)
                     /locus_tag="PK518_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRM48128.1"
                     /translation="MKGILSLLKSKVQKPGEDANDSVLFTTAFASLGVDESLLVPWEA
                     RAVEVGAKRLPRNRGGKLLQSLWAKDKYMAHLDQGAVERMERFFQFASIPPNRDVIRQ
                     DEYGNFMVVLLTGTIAVDRIQPWGEQLRLAETRPGDILGEMSLLDSGMRFSACTTLTD
                     CEVAVLSAEAMDEMMAQDPQLAASLVALLARKLSLRLRVVSARLSENQR"
     gene            complement(1290..2333)
                     /locus_tag="PK518_04125"
     CDS             complement(1290..2333)
                     /locus_tag="PK518_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRM48129.1"
                     /translation="MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDH
                     KTVHAMVYDIMSDAQRKAYEEFLEVDFSFEIDGLARFRVNAFNQNRGAGAVFRTIPSK
                     ILTLEQLNTPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNESEYGHILTIED
                     PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRVIDVFPAEEKEMVRAMLSESLVAVISQTLCKLKDGSG
                     RVAAHEIMTGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQNLTDLVRRNIISPAE
                     ARSKAKIPDNFPG"
     gene            2381..3109
                     /locus_tag="PK518_04130"
     CDS             2381..3109
                     /locus_tag="PK518_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRM48130.1"
                     /translation="MTTIADKLQSVRARIAQACAAAGRAPDSVALLAVSKTFGADAVH
                     AAALAGQCCFGENYIQEAVEKIAQVAALGLPQPLEWHCIGPVQSNKTRLVAEHFDWVH
                     TLDRLKTAQRLSEQRPAHLPPLQVCIQVNVDGGASKSGVAPVDALQLARAVAQMPRLC
                     LRGVMSIPDEVQGGAAQLAVHLRVRQVFDALRAQGGAGFEAFDTLSLGMTADLEAAVA
                     AGSTMVRVGSGIFGARHYPSSPTA"
     gene            3156..4292
                     /locus_tag="PK518_04135"
     CDS             3156..4292
                     /locus_tag="PK518_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM48131.1"
                     /translation="MPGLLPDIDPDGLLEFSVVYTDRALNHMSKRFTGVMQDILAALK
                     EVYNAHTAVLVPGSGTFGMEAVARQFANREKVLIVRNGWFSYRWSQIFDAGGLGGGAV
                     VCKARPQGAGAQAPWAPCPAAEVAAAIRAERPKVVFAPHVETASGIMLSDDYIRSVAD
                     AAHEVGALFVLDCVASGAMWVDMEKTGVDVLISAPQKGWSSSPCCAMVMLSERARAAI
                     EGTTSSSFSCDLKKWMQIAEGYEKGQHAYHATMPTDALVRLRDVMLETRAYGFDKVRA
                     EQMELGAKVRALLESRGFPSVAAEGWKAPGVVVSYTQDAGIQSGKKFLDVGLQTAAGV
                     PLQCDEGPDFKTFRIGLFGLEKWHNVDRTVGHLAAALDKVAAAG"
     gene            complement(4302..5000)
                     /locus_tag="PK518_04140"
     CDS             complement(4302..5000)
                     /locus_tag="PK518_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRM48132.1"
                     /translation="MDFSASFLLAAFGKSLLFALAGVLPIINPLASAPVFVDFTRGLS
                     DDGRARLARRIGKNAALLLAGAMLIGSYLLNFFGVSLPVVRVAGGMVVASIAWRMLHA
                     QQGASDDTTQMAQSLNEDRVRIKAFYPLTFPLTCGPGSIAVAIAIGASLHTKRSVTET
                     MTNIAGGLAAALLLGMLVALTFRYASRLLQRLGETGQIVFLRLMAFMLLCVGVEIIWD
                     GARALLIGIMATGG"
     gene            complement(5027..6538)
                     /locus_tag="PK518_04145"
     CDS             complement(5027..6538)
                     /locus_tag="PK518_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRM48133.1"
                     /translation="MAYSRAMVDTSAPPASERAARQRDVVQALSQCVPAHALLWQAED
                     TTPYECDGLTAYRQRPLVVCLPETEEQVQAVLKACHRLQVPVVARGAGTGLSGGAMPH
                     AMGVTLSLAKFNRILDVDPVARTARVQCGVRNLAISEAAAPHGLYYAPDPSSQIACTI
                     GGNVAENSGGVHCLKYGLTVHNVLAVRGFTMEGEPITFGGQALDAPGYDLLAAMIGSE
                     GMLAVALEVTVRLIPSPQLARCIMASFDDVRKAGDAVAAVIAAGIIPAGLEMMDKPMT
                     AAVEDFVKAGYDLTAAAILLCESDGTPEEVEEEIARMSEVLRAAGATAIAVSRDEQER
                     ARFWSGRKNAFPASGRISPDYMCMDSTIPRKRLADILIAIAEMEKKYQLRCCNVFHAG
                     DGNLHPLILFDANDPDQLHRCELFGADILETSVAMGGTVTGEHGVGVEKLNSMCVQFT
                     AEENAQMLALKKAFDPAGLLNPGKVIPTHNRCAEYGKMLVRGGRLSHPDLPRF"
     gene            complement(6655..7548)
                     /locus_tag="PK518_04150"
     CDS             complement(6655..7548)
                     /locus_tag="PK518_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM48134.1"
                     /translation="MATAFNASAARRIPPIQCLLTFEALARLRSVTQTADELCVTPSA
                     VSHRVKQLEQILGTRLFGRADFSLTTEGSGYLAQVREGLSALQRLPGHAVNPGRRRLK
                     LAVTPTFARSILIPRLRQFTEAYPEIDLALQVSIPLLDVVAEDADLVVRYGPGHYADM
                     EHVEIARDMLTPLASPAFVREHGPFERPEDLEGLPLLRSPLEPWRTWFAAAGLGWPEP
                     NEGSQFNDVGLMCDAAAAGMGVAPVRLKLAAPWLDQGTLVRLFDILAPSPYAHYLCWR
                     TGTMDRWECSAFADWLRRTMG"
     gene            complement(7570..8418)
                     /gene="purU"
                     /locus_tag="PK518_04155"
     CDS             complement(7570..8418)
                     /gene="purU"
                     /locus_tag="PK518_04155"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRM48135.1"
                     /translation="MTPAYILTLSCPDRLGLVHAVSGFLLEHGGNIEEAAQYNDDATG
                     LFFMRVQFACGQHDHAALKTRLASFAEPFAMRWSLHAKSAAMPTVLMVSREGHCLNDL
                     LFRVKSGLLPIDVRAIISNHRDFYQLAASYNIPFHHIPVSAATKAQAEARQYEIIEAE
                     GAELVILARYMQVLSDDLCTKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATA
                     HYVTADLDEGPIIEQDVTRAEHTDTVDDLTSRGRDTESLVLARAVKWHSEHRVLLNGH
                     KTVVFR"
     gene            8514..10784
                     /locus_tag="PK518_04160"
     CDS             8514..10784
                     /locus_tag="PK518_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM48136.1"
                     /translation="MALYWVLGAAWVAAGDALLAYCVTEPQQLAYWQTLKGWLYVLLT
                     GALAWWLLARMSRGQGEIGAARADLLHSENVLRLALEGSGSGLWDWDLLRRRSTHSPG
                     LVRLLRYQGAELPRGMNLLRRLHPDDYRRVHRAVQRAMATGEPFAETARLRRFDDSYC
                     WFQARGMCHRGAQGQAERFSGILTDLTASRALQERQRLAATVVDNTIEGVVVTDAQPC
                     ILSINPAVTRMLGYTEQEVLGQNPRIFQSGRHDKGFYQAMWAAVQRSGHWQGEIWNKR
                     KNGEVFPERMSLSAVHDEEGQVTHYVCMFSDISQEKAQHQRLEFLTQRDSLTGLPNRA
                     WFIEQLDEAMNQALADGEQLAVLLLNLDRFKDVNDSYGHAVGDEVLRHVAQQVRLSLR
                     PGDLLGRMAGDEIAVLVRHLRNTEGAAAVARHLIAATRKPWRTPDGMEVVAGVSIGVA
                     MFPRHASKAEQLLQAAHSAVYGAKAHGRSAHCFFDESMIQAARERLEIEARLRSAMAR
                     GHLLLHYQPQLSVATGRIVGAEALLRWNDPEEGLISPARFIPVAETSGVIGPLGQWVM
                     QEACRQGQAWRAAGLPAIRIAVNVSPRQFQLDDMAVCAAQALAASGLPAHCLELELTE
                     SVLAERPEEMRQVLQRLRELGVRIAVDDFGTGYSSLVHLKRFPIDVLKIDQGFMRDIP
                     ASGDDMAISATIIAMGRSLGLNVLAEGVETPEQLAFLRERGCDSYQGYLFSRPVPAEQ
                     FARLLQAQAEKQPAQA"
     gene            complement(10794..11666)
                     /locus_tag="PK518_04165"
     CDS             complement(10794..11666)
                     /locus_tag="PK518_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRM48137.1"
                     /translation="MRTSKPSVPSPWARACAPAWLSLVLLALPGCALQPPSTPADSAW
                     QAELERWPASDALLLGEQHDAPEHQRWEADSVRWLAERGRLAALVIEMAEAGTGTDGL
                     PPDANAAQVYAALRWNEAAWPWERYRAVIMAAVAAGVPVRGGNLPRSRMRAAMQDEAL
                     DRHLPPAALALQRNAIEEGHCGLLPADRVMPMVRIQLARDASMAQAVQQARQDGRTVL
                     LAAGWGHVRRDLGVPTWLPANFNAKVAIAQAGQARAAIESEANYIHPTPALAPRDHCA
                     ELRARPPGRLPAAK"
     gene            11896..14334
                     /locus_tag="PK518_04170"
     CDS             11896..14334
                     /locus_tag="PK518_04170"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRM48138.1"
                     /translation="MQREITDLAPSLPSQPISLDVLQEKYFKQGESDVEDLYRRVARA
                     LASVEREDIREAMQARFLDNLRAGAIGAGRIMSAAGTDIQATLINCFVQPVGDCIQGV
                     DDAGYPGIYEALREAAETMRRGGGVGYDFSRIRPRGAMVKGTASMASGPCSYINVFDQ
                     SCSTVESAGARRGAQMGVLRIDHPDVHDFITAKRTPGRWNNFNVSVGVPDTFMQAVES
                     DGDWELVHAAAPGADAMKEGAYQRADGQWVYRKLRARELWDTIMKSAYDFAEPGILFL
                     DQINTDNNLGYTETISATNPCGEQPLPPYGCCDLGPIILTRFVRNPFGLHGEAAFDFD
                     AFERSVATQVRALDNVLDVTFWPLPQQQAESAAKRRIGVGFTGMGNTLAMLKLRYDRQ
                     DGRDMAVQIAERMRNAAYRASIELAKEKGAFPKFKADGYLAEGTFASRLPEDIKKQIK
                     KHGIRNSHLLSIAPTGTVSLAFADNASNGIEPPFSWTYTRRKREADGSKSEYVVEDYA
                     WRLYKTLGGDVKKLPAYFVSAMDMAAADHVAMMEAVQPYVDTAISKTVNVPADYPYDD
                     FKNLYLQAWHSGLKGLATYRPNSILGAVLEVPAAEAKPEPKVEPKPEPAAPIDPMRIV
                     IESRPKGGLDAVAEKIEYWTQEGQQRLYLIVSFLPVPDGRGGQVDRAIEFFMPVGQSS
                     ESQQWITSSMRLLSLAARGGFLERALSDMRKVAWDRGPVRLGTFTRADGAQVPLWHDS
                     EVAAVAYAVQSILARRAEAPQQQLLPLDEPEMPAGNPPAMAGKKCGECGAHAVIRKDG
                     CDYCTQCGAIGSCG"
     gene            14410..16309
                     /locus_tag="PK518_04175"
                     /pseudo
     CDS             14410..16309
                     /locus_tag="PK518_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
     gene            16306..16974
                     /locus_tag="PK518_04180"
     CDS             16306..16974
                     /locus_tag="PK518_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM48139.1"
                     /translation="MSAPITLFLIDDHTLLRRGLVALLGQHADLRVVGEAGDAGQALR
                     LLPVLRPDVILLDNHLPGVRGVDAIADLREASPASRVIMLTVSEDGEDLASALRHGAQ
                     GYLLKTIDGDLLAQAVRRAASGEPVVSPEMMGKLVAAFQSQGAPEPEPAPEEAEPAPQ
                     LSPREEEVLREIARGASNKEIARALDIAETTVKIHVQHILRKLGLSSRVQAAVYASDR
                     QRAE"
     assembly_gap    17130..17229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17231..17572)
                     /locus_tag="PK518_04185"
     CDS             complement(17231..17572)
                     /locus_tag="PK518_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48140.1"
                     /translation="MSQAQGQRAGDPGPVEGASRARENYLRLLAWAFTLFSSVRILGY
                     LPTAWAILASGSSQQHSLLTWAIWTSSNMTMAAWLYEDNGRRLNRAIVVNIGNALMCL
                     VILALVAFYRH"
     gene            complement(17569..17994)
                     /locus_tag="PK518_04190"
     CDS             complement(17569..17994)
                     /locus_tag="PK518_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48141.1"
                     /translation="MNRILLCATLGMAACMGACAQQPSGQSTPLFDDEGRPAANVPAA
                     IPASPAHRTRAGLYITEAQARAHEQTLPGGAISVFAGCCGDRGLDDAMVSAWAQHASL
                     DAPSDMPVLVRGSDLQQAARLADRLTDAGFAPVFLVSVP"
     gene            complement(18044..19141)
                     /locus_tag="PK518_04195"
     CDS             complement(18044..19141)
                     /locus_tag="PK518_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48142.1"
                     /translation="MPGTVRKYRYQSTRPGTPIRPLDDAPGALGGDDDAIDNHEEESA
                     MTLDRRTLCLAAMAALCNVPLARAADYPSRAITIVVPTPAGGASDSAARLVAASLSAA
                     WGQPVVVVNRPGAGGAIAAQAVMDAPPDGYTLLWGLSAMAGLPLVQKGVPYKALSEFT
                     PVANVVELGFALFVNRELPVQNFADLVAYGRSHPGQLHLATGTLGEYMLAERVLKAAG
                     VQARRVPYKGGAQLMPDLISGQVQINFGPIAGGLQHAKAGKLRVLATALPQRSPLLPD
                     VPTFAELGVSSGTLSFWNALFAPAATPPEVAARLSAAVARALQAPAVQEPLRASGTEP
                     LGSTPQQLAEAVEAASTAWATFVRDYGIAQE"
     gene            19169..20182
                     /locus_tag="PK518_04200"
     CDS             19169..20182
                     /locus_tag="PK518_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48143.1"
                     /translation="MTSAHHLSPMGRRRLMAAAAGIALLPPLIPPVQAQPSRTARIGW
                     LGWIGAAGAAASALALAAFRAGLADRGWAEGRGYELLVRDGDQPRSADLAAELVRSGV
                     DLLVAQGPMVFGARKVSGATPVIFNIGGDPVEAGLVASLSHPGGHLTGVTSLSTELAG
                     KRLELLKTAMQWGSHVAAIANALHAGFTIERDAVQAAARRLGLTLSWHLLKSMDDFDT
                     ALPRIASEGAHALLAVPDNLINVRAQTLARFSAAQRLPSISGWAAFAEAGNLMSYGPN
                     VHGYYRQMAAIADRLLRGARAADIAVEQPRDIELVVNAKVARALGLDLPAELQLRADR
                     WIE"
     gene            20160..22388
                     /locus_tag="PK518_04205"
     CDS             20160..22388
                     /locus_tag="PK518_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM48144.1"
                     /translation="MPTAGSNERPWPGWWRSALGRRYAAVFALLVSLPLLAYAAWSAA
                     QVYRQQRDALAAVQTAQADAAAARIQRFLREIELQLSWLTALPWTPDGAPQRRLDALR
                     ALRQTPAIADIALIDGQGRERVAESRLALGRVDSMADRMASPAFSGARQARGAYFGDV
                     SFRKGSEPFMTVAVAGPRGEAGVAIAEVSLKYIWDVVSEIRIGDEGIVYVLDPAGRLI
                     AHPDINLVLRNTDLSATLRAFENQKAAAPPRPALRLVGTRGEPVLASSVMLAPPGWRV
                     VVEQGLQEADAPLWANVQGALWVAAVSLLLALSAALWSAWRIARPLRELAQGAERIGA
                     GQLSHRIALHSGDELERLGERFNSMAAELQASHDNLGRQVEERTRELSDANRAKSRLL
                     AAASHDLRQPLHALNLMVAQWRRDPDAAERRRLGLRVEQAIAAINALFDGLLDVSKLD
                     AGVIRPQVEAFPVQRLLDRMDVAHAAAARDKGLELRIRPCDAWVASDPVLLERILGNL
                     VANAVRYTRSGAMLLGCRRTRAGLRIAVWDTGIGIAPDKQERIFDEFYRLDAEGGAAD
                     GGLGLGLSIVARLAGLLGHQVSVRSVPGRGSCFAVTVPETASQPHLPTAANDSAIDAL
                     QGRRVLVIDNNAQVLASTARLLEGWGCCVLAAHGAPDHVHGPVDLLLVDMRLDHGDDG
                     VAAISRLRRQWGRDVPALILTGDTAIGTRERIAASGLPMLEKPVSALRLRSALTRLLQ
                     RG"
     gene            complement(22376..23020)
                     /locus_tag="PK518_04210"
     CDS             complement(22376..23020)
                     /locus_tag="PK518_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM48145.1"
                     /translation="MNFLVVDDHPLVREALASLLLELRPGSRVQQAASGAEAITALGC
                     GQAVDLVLLDLRLPDGSGLDLLRRIGAASIGTAVVMLSGDLDRQTVQEALAAGAVGYV
                     PKTEPREIIAGALGLVLAGGVYVPPVALQTAARTFDTRAATPEALGLTGRQLEVLALL
                     MQGKNNKLICRALGLAEPTVKNHVSAILRALGVDSRTEAVLAVTRLGWALPQPR"
     gene            23272..24567
                     /locus_tag="PK518_04215"
     CDS             23272..24567
                     /locus_tag="PK518_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HRM48146.1"
                     /translation="MAPASHSAAQDPRRNRQAWSVLIVSTLAFTVCFMVWMMFGIIGI
                     PIKKMLDLNATQFGLLTAMPVLTGSLVRVPLGIWTDKYGGRIVMAVLMAITVPAIWVM
                     GYASEYWHFLVIGLFVGLAGGAFSVGTPYVARWFPRHRQGTAMGVYGAGNSGAAVNKF
                     VAPVILVAFGWAAVPHVYAAIMLGALLLFWFFSHSDPSHLVASNISFTDQLKALKDPR
                     VLKYCQYYSIVFGGYVALSLWMVQYYVGEYGLDIRVAALLAACFSLPGGVLRAIGGVL
                     SDRYGAHSVTWWVMWVSWICLFLLSYPQTAFTIQTVNGPSTFHIGLNVYVFTAVMAVL
                     GVAWAFGKASVFKYISDDYPGNIGAISGIVGLAGGMGGFILPIMFGALMDWTGIRSSA
                     FMLMYGVVWVSLIWMYWTEVRRTDVMTGQGVAAATPAAR"
     gene            24588..26033
                     /locus_tag="PK518_04220"
     CDS             24588..26033
                     /locus_tag="PK518_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM48147.1"
                     /translation="MSADTVAQEGARQGRLLHIWTPEDKQFWEREGEAVAKLNLWISV
                     PALFLAFAVWQVWSVVAVNLPALGFKYSTNQLFWLAAAPALSGATLRIFYSFMVPVFG
                     GRRWTALSTATLLIPMLGIGFAIRDNTTGYPTMLMLALLCGLGGGNFSSSMANISFFF
                     PKERKGSALGVNAGLGNLGVSVVQFLSPLVISLGIFGIFGGDAQVIVRNGEQQHVWAQ
                     NAAFIWVPWIAITAVVAWFGMHDIADAKASFAAQAAIFRAKHNWIMCLLYLGTFGSFI
                     GFAAGFPLLMKALFPAINPLAYAWLGPLVGALTRPFGGWLADKIGGGVVTFWNFIVMA
                     LAVLGVLFFLPKGASAYALPFGPAEGSFTGFFLMFLVLFCTTGIGNGSTFRMIPVIFL
                     TQRLRALRGNDEAAQAQAIKDGNTEGAAAVGFAGALGAYGGFFIPKSYGSSIASTGGP
                     EFALWMFVVFYSLCVVVTWWYYARRNAEMPC"
BASE COUNT         4042 a         8620 c         9067 g         4248 t
ORIGIN      
        1 gtgtccaggc ccacctcgcg ctgcgtgacc acgcagttct tgtgcgggtg cacgaactcg
       61 atcgggtctt cgacggtgat gatgtggccg aaggagttct cgttgcgcca gtccaccatg
      121 gccgccagcg tggttgactt gcccgaaccc gtggcgccca ccaggatgca caggccgcgc
      181 ttggccaggg cgatctcctt gagcacctgg ggcacgccca ggccgtctat ggtcggcagg
      241 gtcagcggaa tcgtgcgcag caccatgccc acgcggccct gctggatgaa ggcgttggcg
      301 cgaaagcggc caatgcccgc gggcgatatc gcaaagttgc actccttggt gcgctcgaag
      361 tcggcgatct ggcggtcact catgatggag cgcgccagcg tcagcgtatg cgccggcgtc
      421 agcggctgtg gcgagacctt ggtgaccttg ccatcgacct tgatcgccgg cggaaactcg
      481 gccgtgatga acaggtcgct gccgccgcgg ctgaccatca gtttgagcag gtcgttgatg
      541 aatttgctgg cctgatcgcg ttccattgca ttcctccccg tgcctttgtt tcagcgctgg
      601 ttttcgctca ggcgcgcgct caccacgcgc aggcgcagcg acagcttgcg tgccagcagg
      661 gccaccaggc tggccgccag ctgcgggtct tgcgccatca tctcgtccat ggcctcggcg
      721 ctgagcacgg ccacttcgca gtcggtcagc gtggtgcagg cagaaaagcg catgccgctg
      781 tcgagcagcg acatctcgcc caggatgtca cccgggcgcg tctcggccag gcgcagttgc
      841 tcgccccagg gctgaatgcg atcgacggcg atggtgcccg tgagcagcac caccatgaag
      901 ttgccgtact cgtcctggcg gatcacatcc cggttgggcg ggatggaggc gaactggaag
      961 aagcgctcca tgcgctcgac cgcgccctgg tccagatgcg ccatgtactt gtccttggcc
     1021 cacagcgact gcagcagctt gccgccgcgg tttctgggca ggcgcttggc gccaacctcg
     1081 acggcgcgcg cctcccaggg gacgagcagc gattcatcca cccccaggct ggcaaatgcg
     1141 gtggtgaaga gcaccgagtc gttcgcgtct tccccgggct tttgcacctt ggatttgagc
     1201 aggctgagaa tgcctttcat ggacggactc cgatacgaga gaggcgcgcg aacgcgggca
     1261 aaacgggcga gcgggctgat ggcatggcat caacccggga agttgtccgg aatcttggcc
     1321 ttgctgcgcg cctcggccgg gctgatgatg ttgcgccgca ccagatccgt caggttctga
     1381 tccagcgtct gcatgcccac gctgttgctg gtctgtatgg tggagtacat ctgcgccacc
     1441 ttggcctcgc ggatcaggtt gcgaatggcg ctggtgccgg tcatgatctc gtgcgccgcc
     1501 acgcggcccg agccgtcctt gagcttgcac agcgtctgcg aaatcacggc caccaatgac
     1561 tccgacagca tggcgcgcac catctccttt tcctcggcgg ggaagacgtc gatcacgcgg
     1621 tcaatggtct tggcggcgct ggacgtgtgc agcgtgccaa acaccaggtg gccggtctcc
     1681 gctgccgtca tcgccaggcg tatggtctcc aggtcgcgca gctcgcccac caggatggcg
     1741 tccgggtctt cgcgcagggc tgaacgcagc gcgttggaaa acgatagcgt catcggcccg
     1801 acctcacgct ggttgatcag gcatttcttc gattcgtgca cgaattcgat cgggtcctcg
     1861 atggtcagga tatggccgta ttcggattcg ttcaggtggt tcaccatcgc cgctagcgtg
     1921 gtggacttgc ccgaacccgt ggggcccgtc accagcacca ggccacgggg cttgagcgcc
     1981 agatcggcaa atatcttggg cgtgttcagc tgctccagcg tcagaatctt gctgggaatg
     2041 gtacggaaca cggcgcccgc gccacggttc tggttgaagg cgttgacgcg aaagcgcgcc
     2101 aggccgtcga tctcgaagga aaagtccacc tccaggaact cttcataggc cttgcgctgg
     2161 gcatcgctca tgatgtcgta caccatggcg tgcacggtct tgtggtccag cgcatcgaca
     2221 ttgatgcgcc gcacatcgcc gtgcacccgg atcatgggcg gcaggccggc cgacagatgc
     2281 aggtcagagg ccttgttctt cacgctgaag gcgagcaact gggtgatgtc cacgggatcc
     2341 ctttgggtcg tttgatacgc ttggtaaaag attacacatt atgacgacga ttgcagacaa
     2401 gctccagagc gtgcgtgcgc gcatcgcaca ggcctgcgcc gcggcagggc gcgcgcccga
     2461 ctccgtggcc ctgctggccg tgtccaagac ctttggcgcc gatgcggtgc acgcggcggc
     2521 gctggccggc cagtgttgtt ttggcgagaa ctacatccag gaggcggtgg aaaaaatcgc
     2581 ccaggtggcc gcgctgggcc tgccccagcc gcttgaatgg cactgcatag gcccggtgca
     2641 gagcaacaag acacgcctgg tggccgagca tttcgactgg gtgcacacgc tggacaggct
     2701 gaagacggcg cagcgactgt cagagcagcg cccggcgcat ctgccgccgc tgcaggtgtg
     2761 catacaggtg aatgtggacg gcggcgccag caagtccggt gtggcgcccg tagatgcgct
     2821 gcagttggcg cgcgccgtgg cgcaaatgcc acgtttgtgc ctgcgcgggg tgatgagcat
     2881 tcccgacgaa gtacagggcg gggcggcgca gctggccgtg catttacggg tgcgccaggt
     2941 gttcgatgca ctgcgcgccc agggcggtgc cgggttcgag gccttcgaca ccctgtcgct
     3001 gggcatgacc gccgatctgg aggccgccgt ggccgctggc agcaccatgg tgcgcgtggg
     3061 cagcggcatc tttggggcac gccactaccc ttcaagcccc acggcctgag gtgctaagct
     3121 gcgccgctgt tgctattttg caaggagaca cccccatgcc cggcctgctg cccgacatcg
     3181 atcccgatgg cctgcttgaa ttctcggtgg tctataccga ccgcgcgctc aaccacatgt
     3241 ccaagcgttt caccggggtg atgcaggaca ttctggccgc gctgaaagag gtctacaacg
     3301 cccacacggc cgtactcgtg cccggcagcg gcacctttgg catggaggcc gtggcgcgcc
     3361 agttcgccaa ccgcgagaag gtgctcatcg tgcgcaacgg ctggttcagc taccgctgga
     3421 gccagatctt cgacgccggc ggcctgggcg gtggtgccgt ggtgtgcaaa gcacgcccgc
     3481 agggtgcggg cgcgcaggcg ccctgggcgc cctgcccggc ggctgaagtg gcggccgcca
     3541 tccgcgccga acgtccgaag gtggtgttcg ccccgcatgt ggagacggcc agcggcatca
     3601 tgctcagcga tgactacatc cgcagcgttg ccgacgccgc gcacgaggtg ggcgcgctct
     3661 tcgtgctcga ctgcgtggcc tcgggcgcga tgtgggtgga catggaaaag accggcgtgg
     3721 acgtgctgat ttccgcgccg caaaagggct ggagcagttc gccttgctgc gccatggtga
     3781 tgctgtccga gcgtgcgcgc gcggccatcg agggcacgac cagcagcagc ttctcctgcg
     3841 atctgaagaa gtggatgcag attgccgagg gctacgaaaa aggccagcat gcctaccacg
     3901 ccaccatgcc caccgatgcc ctggtgcgcc tgcgcgacgt gatgctggag acgcgcgcct
     3961 atggctttga caaggtgcgt gccgagcaga tggagctggg cgccaaggtg cgcgcgctgc
     4021 tggaatcgcg cggttttccc agcgtggcgg ccgagggctg gaaggccccg ggggtggtgg
     4081 tgagctacac ccaggatgcc ggcattcaga gtggcaagaa attcctcgac gtgggtctgc
     4141 agaccgccgc cggcgtgccg ctgcagtgcg acgaggggcc ggacttcaag accttccgca
     4201 tcggcctgtt cggcctggag aaatggcaca acgtcgatcg cacggtgggc catctggcgg
     4261 cggcgctgga caaggtggcc gccgccggct gacgcgccgg cctacccgcc tgtggccatg
     4321 atgcctatca gcagggcgcg cgcgccatcc cagatgatct ccacgcccac gcacagcagc
     4381 atgaaggcca tcaggcgcag aaacacgatc tgcccggtct cgcccaggcg ctgcagcagt
     4441 cggctggcgt agcgaaaggt cagcgccacc agcatgccca gcagcagggc ggcggccagg
     4501 ccgccggcga tgttggtcat ggtttccgtc acgctgcgtt tggtgtgcag gctggcgcca
     4561 atggcgatcg ccacggcgat ggagccgggg ccgcaggtga gcggaaaggt cagcgggtag
     4621 aaggccttga tgcgcacccg gtcctcgttc agcgactggg ccatctgtgt ggtgtcgtcg
     4681 ctggcgccct gctgggcgtg cagcatgcgc caggcgatgg aggccacgac catgccgccg
     4741 gccacgcgca ccaccggcag cgacacgcca aagaagttca gcaggtagga gccgatcagc
     4801 atggcccctg ccagcagcag cgcggcgttc ttgccaatgc gccgcgccag ccgggcgcgg
     4861 ccgtcgtcgc tcaggccgcg cgtgaagtcc acgaacaccg gtgccgaggc cagtgggttg
     4921 atgatgggca gcacgccggc cagcgcaaac agcaggcttt tgccgaaggc ggcgagcaaa
     4981 aacgaggcgg aaaaatccat gggcgctact gtacgcgccg cagcggtcaa aaacgtggca
     5041 aatcggggtg gctgagcctg ccgccgcgca ccagcatctt gccgtactcg gcgcagcggt
     5101 tgtgcgtggg gatcaccttg ccggggttga gcagcccggc cgggtcgaag gcctttttca
     5161 gggccagcat ctgcgcgttc tcctcggccg tgaactgcac gcacatgctg ttgagctttt
     5221 ccacgcccac gccatgctcg cccgtcaccg tgccgcccat ggccacgctg gtctccagga
     5281 tgtcggcgcc aaacagctcg cagcggtgca gctggtcggg gtcgttggca tcgaacagga
     5341 tcagcgggtg caggttgccg tcgccggcgt ggaacacgtt gcagcagcgc agctggtact
     5401 ttttctccat ctcggcgatg gcgatcagga tgtcggccag gcgcttcctc ggaatggtcg
     5461 aatccatgca catgtagtcg gggctgatgc ggccgctggc cgggaaggcg ttcttgcgcc
     5521 cgctccagaa gcgcgcgcgc tcctgctcgt cacggctcac ggcaatggcg gtggcgccgg
     5581 cggcgcgcag cacctcgctc atgcgcgcga tctcttcctc gacttcctcg ggggtgccgt
     5641 cgctctcgca cagcaggatg gccgcggccg tcaggtcgta gccggccttg acaaaatctt
     5701 ccacggcggc ggtcatgggc ttgtccatca tctccaggcc ggccgggatg atgccggcgg
     5761 cgatcaccgc ggccacggcg tcgccggcct ttcgcacatc gtcaaagctg gccatgatgc
     5821 agcgcgccag ctgtgggctg gggatcaggc gcaccgtcac ctccagcgcc acggccagca
     5881 tgccctcgct gccaatcatg gccgccagca ggtcgtagcc gggcgcgtcc agcgcctggc
     5941 cgccgaaggt gatgggctcg ccctccatgg tgaagccgcg caccgcgagc acgttgtgca
     6001 ccgtcaggcc gtatttcagg cagtgcacgc cgccggagtt ttcggccacg ttgccgccaa
     6061 tggtgcaggc gatctggctg ctggggtcgg gggcgtagta caggccatgg ggggcggcgg
     6121 cctcgctgat ggccaggttg cgcacgccgc attgcacgcg cgccgtgcgc gccaccggat
     6181 cgacatcgag gatgcggttg aacttggcca gcgacagcgt cacgcccatg gcgtgcggca
     6241 tggctccgcc cgacaggccg gtgccggcgc cgcgcgccac caccggcacc tgcagccggt
     6301 ggcaggcctt gagcacggcc tgcacctgct cttcggtctc gggcaggcag accaccagcg
     6361 ggcgctggcg gtaggccgtc aggccatcgc attcataggg cgtggtgtcc tcggcctgcc
     6421 agagcagcgc atgggcgggc acgcattggg acagggcttg cacaacgtcg cgctggcgcg
     6481 cggcgcgttc gctggcgggg ggggcggagg tatcgaccat ggcgcgactg taggccatgg
     6541 catggggcca tggcgtgaat cttcttgcaa gccgccgtga agtcgccgat agctataagt
     6601 ataatagctg tatgcgcttg ctggataagt gcttgcggcc gatttttgtt gaaatcaccc
     6661 catggtccgg cgtagccagt cggcaaaggc cgagcattcc cagcgatcca tggtgccggt
     6721 acgccagcac aggtaatgcg cgtagggact gggtgccagg atgtcgaaca ggcgcaccag
     6781 cgtgccctgg tccagccagg gcgcggccag cttcaggcgc accggtgcca cgcccatgcc
     6841 ggcggcggcg gcgtcgcaca tcaggccaac gtcgttgaat tgcgagccct cgttgggctc
     6901 gggccagccc aggccggcgg cggcaaacca ggtgcgccag ggctccagcg ggctgcgcag
     6961 caggggcagg ccctccagat cctcggggcg ctcgaacggc ccatgctcgc gcacaaaggc
     7021 gggcgaggcc aggggcgtga gcatgtcgcg cgcgatctcc acatgctcca tgtcggcgta
     7081 gtggccgggg ccgtagcgca ccaccaggtc ggcgtcctcg gccaccacgt ccaggagcgg
     7141 aatcgacacc tgcagcgcca ggtcgatctc ggggtaggcc tcggtgaact ggcgcaggcg
     7201 cggaatcagg atggagcgcg caaaggtcgg cgtgacggcc agcttcagcc gtctgcggcc
     7261 agggttgacg gcatggcccg gcaggcgctg cagggccgac aggccctcgc gcacctgggc
     7321 caggtagccg ctgccctcgg tggtcagcga aaaatcggcg cgcccgaaca gccgcgtgcc
     7381 caggatctgc tcgagctgct tgacgcggtg gctcacggcg ctgggcgtca cgcacagctc
     7441 gtcggcggtc tgcgtcacgc tgcgcaggcg cgccagcgcc tcgaaggtca gcaggcactg
     7501 tatcggcggt atgcgccgtg cggcgctggc gttgaaggcg gtggccatgg ccggcaatgc
     7561 ggcaggggtt tagcggaaga ccacggtctt gtggccgttg agcagcacgc ggtgttcgct
     7621 gtgccatttc acggcgcgcg ccagcaccag gctttcggtg tcgcgcccgc gcgacgtcag
     7681 gtcgtccacg gtgtccgtgt gttcggccct tgtaacgtct tgttcgataa tcggcccttc
     7741 gtcgaggtcg gcggtgacgt agtgagcggt tgctcctatc aacttcacgc cccggtcatg
     7801 ggcctggtaa tagggcttgg cgcccttgaa gctgggcaga aagctgtggt ggatgttgat
     7861 ggcgcggccg gccaatttgg tgcacagatc gtcggacaag acctgcatgt agcgcgccag
     7921 tatgaccagc tcggcaccct cggcctcgat gatttcgtat tgccgcgcct cggcctgggc
     7981 cttggtggcg gcgctgacgg gtatgtggtg aaagggaatg ttgtagctgg cggccagttg
     8041 gtagaagtcg cggtggttgc tgatgatggc gcgcacatct atcggcagca ggccgctttt
     8101 gacgcggaac agcaggtcgt tcaggcaatg gccctcgcgg ctgaccatga gcacggtggg
     8161 catggcggct gatttggcgt gcaggctcca gcgcatggca aagggctcgg caaagctggc
     8221 caggcgggtc ttgagcgcgg cgtggtcatg ctggccgcag gcgaactgca cgcgcatgaa
     8281 gaacagaccg gtggcgtcgt cgttgtattg cgcggcttcc tcgatgttgc cgccatgctc
     8341 gagcaaaaag cccgagacgg cgtgcaccag ccccaggcgg tcgggacagg acagggtaag
     8401 aatgtaagcg ggggtcattg gcgcattgtc gcagcagccc tcggaagata agcagagtca
     8461 ttgtgaaatc gattgcatcg cgaattgaca cgggaatcag cccctggtgg ggcatggccc
     8521 tgtattgggt tctgggcgcg gcctgggtgg ccgcgggcga cgccttgctg gcctattgcg
     8581 tcaccgagcc gcagcagctg gcctattggc agaccctcaa gggctggctg tatgtgctgc
     8641 tgaccggggc gctggcctgg tggctgctgg cgcgcatgtc gcgcggccag ggggagattg
     8701 gcgccgcgcg cgccgacctg ctgcacagcg aaaacgtgtt gcgcctggcg ctggaaggca
     8761 gcggcagcgg cctgtgggac tgggacttgc tgcgccgccg ctccacccat tcgcccggtc
     8821 tggtgcgcct gctgcgctac cagggcgccg agctgccgcg cggcatgaat ctgctgcgcc
     8881 gcctgcaccc ggatgactat cggcgcgtgc accgggccgt gcagcgggca atggccacgg
     8941 gcgagccgtt tgccgagacg gcgcgcctgc ggcgctttga cgacagctat tgctggttcc
     9001 aggcgcgcgg catgtgccac cgtggcgcac aggggcaggc cgagcgtttc tccggcatcc
     9061 tcaccgacct cacggccagc cgtgcgctgc aagagcgcca gcgcctggcg gcgaccgtgg
     9121 tggacaacac catcgagggc gtggtggtga ccgatgcgca accgtgcatc ctgtccatca
     9181 acccggcggt cacgcgcatg ctgggctaca cagaacagga ggtgctgggc cagaacccgc
     9241 gcatcttcca gtccggcagg catgacaagg ggttttacca ggccatgtgg gccgcggtgc
     9301 agcgcagcgg ccattggcag ggcgagatct ggaacaagcg caagaacggc gaggtcttcc
     9361 ccgagcgcat gtcgctgtcg gcggtgcatg acgaggaggg ccaggtcacg cactatgtat
     9421 gcatgttcag cgacatctcg caggaaaagg cccagcacca gcggctggaa ttcctcaccc
     9481 agcgcgacag cctgaccggc ctgcccaacc gagcctggtt catcgagcag ctggacgagg
     9541 ccatgaacca ggccctggcc gatggcgaac agctggccgt gctgctgctg aacctggatc
     9601 gcttcaagga tgtgaacgac agctacggcc atgccgtcgg cgacgaggtg ctgcggcatg
     9661 tcgcgcagca ggtgcgcctg agcctgcgcc cgggcgacct gctggggcgc atggcgggcg
     9721 acgagatcgc cgtgctggtg cgccacctgc gcaataccga aggcgcggct gccgtggcca
     9781 ggcatttgat agccgcgacg cgcaagccct ggcgcacgcc ggacggcatg gaggtcgtcg
     9841 ccggggtcag catcggcgtc gccatgttcc ccaggcatgc gtccaaggcc gagcagctgc
     9901 tgcaggcggc gcacagcgcg gtgtatggcg ccaaggccca tgggcgcagc gcgcattgtt
     9961 tctttgacga gtccatgatc caggccgcgc gcgagcggct ggagatcgag gcgcgcctgc
    10021 gcagcgccat ggcgcgcggt catctgctgc tgcactacca gccgcagctg agcgtggcca
    10081 cggggcgcat cgtgggtgcc gaggcgctgc tgcgctggaa cgaccccgag gaggggctga
    10141 tctcgccggc gcgcttcatc cccgtggccg agacctcggg cgtgatcggc ccgctgggcc
    10201 agtgggtcat gcaagaggcc tgccgccagg gccaggcctg gcgcgcggcc ggcctgccgg
    10261 cgatccgcat cgcggtgaat gtctcgccgc gccagttcca gctggacgac atggccgtct
    10321 gcgcggccca ggcgctggcc gccagcggcc tgccggcgca ttgcctggag ttggagctga
    10381 ccgagtccgt gctggccgag cggcccgagg aaatgcgcca ggtgctgcag cggctgcggg
    10441 agctgggcgt gcgaatcgcg gtggacgact ttggcaccgg ctactcctcg ctggtgcacc
    10501 tcaagcgctt tcccatcgat gtgctgaaga tcgaccaggg cttcatgcgc gacatacccg
    10561 ccagcggcga cgacatggcc atcagcgcca ccatcatcgc catgggccgc agcctgggcc
    10621 tgaacgtgct ggccgagggc gtggagacgc ccgagcagct ggcctttctg cgtgaacgtg
    10681 gctgcgacag ctaccagggc tacctgttca gccggcctgt gccggccgag caattcgccc
    10741 ggctgttgca ggcccaggca gaaaagcagc cggcccaggc ctgatccgca gtgctacttg
    10801 gcggcgggca gccggccggg tggacgcgcg cgcagctcgg cgcaatggtc gcgcggcgcc
    10861 agcgccggcg tggggtggat gtagtttgct tctgattcaa tagctgctcg cgcttgtcca
    10921 gcctgcgcta tggccacttt tgcgttgaag tttgctggca gccaggtggg cacgcccaga
    10981 tcgcgccgca catggcccca gccggccgcc agcagcaccg tgcggccgtc ctggcgcgcc
    11041 tgctgcaccg cctgggccat gctggcgtcg cgcgccagct gtatgcgcac catgggcatg
    11101 acgcggtcgg cgggcagcag gccgcagtgg ccttcctcga tggcgttgcg ctgcagcgcc
    11161 agcgccgcgg gtggcaggtg tctgtccagc gcctcgtcct gcatggcggc gcgcatgcgg
    11221 ctgcggggca ggttgccgcc gcgcaccggc acgcccgccg ccacggcggc catgatcacc
    11281 gcgcggtagc gctcccaggg ccaggcggcc tcgttccagc gcagtgcggc atacacctgg
    11341 gcggcgttgg cgtcgggcgg caggccgtcg gtgccggtgc cggcctcggc catctcgatc
    11401 accagcgccg ccaggcggcc gcgctcggcc agccagcgca cgctgtcggc ctcccagcgc
    11461 tggtgctcgg gtgcgtcatg ctgctcgccc agcagcaggg cgtcgctggc tggccagcgc
    11521 tccagctccg cctgccaagc gctgtcggcg ggcgtggatg ggggctgcag ggcgcatccc
    11581 ggcaatgcca gcagcaccag cgacagccat gccggcgcgc aggcacgtgc ccatggggag
    11641 ggcacggagg gtttggaagt acgcatcaaa atgaaaccaa ctgttaactg aaatgcacaa
    11701 tgcagaaacc gcgccgtccc gcgtctgcgg gcgcgctggt gctacaggaa gtgtatggca
    11761 tagtggcgac acgctatata tagcgtttgt caagcctttt tggtgaatgc aaggtatggc
    11821 ctctgggtgc acaatccagc ccctgactgc caacaatccc tccccccttt gaacaccccc
    11881 aggagtttcc tcgccatgca acgtgagatc accgacctcg cccccagcct gcccagccag
    11941 cccatcagcc tcgatgtgct gcaagaaaaa tacttcaagc agggcgagag tgatgtcgag
    12001 gacctgtacc gccgcgtggc gcgggccctg gcatcggtgg agcgcgagga catccgcgag
    12061 gccatgcagg cgcgctttct ggacaacctg cgcgccggcg ccattggcgc cggccgcatc
    12121 atgagcgccg ccggcaccga catccaggcc acgctgatca actgcttcgt gcaacccgtg
    12181 ggcgactgca tccagggcgt ggacgacgcc ggctaccccg gcatctacga ggccctgcgc
    12241 gaggccgccg agaccatgcg ccgtggcggc ggcgtgggct atgacttctc gcgcatccgc
    12301 ccgcgcggcg ccatggtcaa gggcacggcc tccatggcct cggggccctg cagctacatc
    12361 aacgtcttcg accagtcctg ctccaccgtg gagagcgccg gcgcccgccg cggcgcgcaa
    12421 atgggcgtgc tgcgcataga ccacccggac gtgcatgatt tcatcaccgc caagcgcacg
    12481 ccgggtcgct ggaacaactt caacgtctcg gtgggcgtgc ccgacacctt catgcaggcc
    12541 gtggagagcg atggcgactg ggagctggtg cacgccgcgg cccccggcgc cgatgccatg
    12601 aaggagggcg cctaccagcg cgccgacggc cagtgggtgt accgcaagct gcgcgcgcgc
    12661 gaactgtggg acaccatcat gaagtcggcc tacgacttcg ccgagcccgg catcctgttc
    12721 ctggaccaga tcaacaccga caacaacctc ggctacaccg agaccatcag cgccaccaac
    12781 ccctgcggcg agcagccgct gccgccctac ggctgctgcg atctggggcc catcatcctg
    12841 acgcgcttcg tgcgcaaccc gtttggcctg catggcgagg cggccttcga cttcgacgcc
    12901 ttcgagcgct ccgtcgccac ccaggtgcgc gcactcgaca acgtgctcga cgtcactttc
    12961 tggcccctgc cgcagcagca ggccgagtcc gcggccaagc gccgcatcgg cgtgggcttt
    13021 accggcatgg gcaacaccct ggccatgctc aagctgcgct atgaccgcca ggacgggcgc
    13081 gacatggcgg tgcaaatcgc cgagcgcatg cgcaacgccg cctaccgcgc ctccatcgag
    13141 ctggccaagg agaagggcgc cttccccaag ttcaaggccg acggctacct ggccgagggc
    13201 accttcgcca gccgcctgcc cgaggacatc aagaagcaga ttaagaagca cggcatacgc
    13261 aacagccacc tgctgtccat tgcccccacc ggcacggtga gcctggcctt tgccgacaac
    13321 gcctccaacg gcatcgagcc gccgttctcc tggacctaca cgcgcaggaa gcgcgaggcc
    13381 gacggcagca agagcgaata cgtggtcgag gactatgcct ggcgcctgta caagaccctg
    13441 ggtggtgacg tgaagaagct gccggcgtac ttcgtcagcg ccatggacat ggccgccgcc
    13501 gaccatgtgg cgatgatgga ggccgtccag ccctatgtgg acacggccat ctccaagacc
    13561 gtcaacgtgc cggccgacta cccgtacgac gacttcaaga acctctacct gcaagcctgg
    13621 cactcgggcc tgaagggtct ggccacctac cgccccaaca gcatcctggg cgcggtgctg
    13681 gaggtgcccg ccgccgaggc caagcccgag cccaaggtgg agcccaagcc cgagccggcc
    13741 gcgcccatcg acccgatgcg catcgtcatc gaaagccggc ccaaaggcgg gctggacgcc
    13801 gtggccgaga agatcgagta ctggacgcag gagggccagc agcggctgta cctgatcgtc
    13861 tccttcctgc ccgtgccgga cggccgcggc ggccaggtgg accgggccat cgagttcttc
    13921 atgcccgtgg gccagagcag cgagtcgcag cagtggatca cgtcgagcat gcgcctgttg
    13981 tcactggccg cgcgcggcgg cttcctggag cgcgccctgt ccgacatgcg caaggtggcc
    14041 tgggatcgcg gcccggtgcg cctgggcacc ttcacgcgcg ccgacggcgc ccaggtgccg
    14101 ctgtggcacg actccgaggt ggcggccgtg gcctacgccg tgcagagcat cctggcgcgc
    14161 cgcgccgagg cgccgcagca gcagctgctg ccgctggacg agcccgagat gcccgccggc
    14221 aacccgcccg ccatggccgg caagaaatgc ggcgaatgcg gcgcccacgc cgtgatccgc
    14281 aaggacggct gcgactactg cacgcagtgc ggggccattg gcagctgcgg ctgagggcgg
    14341 ccaacagcat cgctgatact attaattaaa tagctgttag cgcttgctgt ataaggggta
    14401 gggcggtcaa tgaataaaaa actgaccctc tccaacaagc tgctcaccat gggcacggcc
    14461 ttcctgctgg tggccctggc gtcgataggc ttcacgctct gggtcacctg ggaactagag
    14521 ggcggcgcgg ccgcggtgaa cgaggccggg cgcctgcgca tgaatatgct gcgcatgatc
    14581 ctggcgcagc aaaccgagtc gccacaggac tttgcgcgcc tggagcagcg cttcgaggcc
    14641 ggcctggagc tgttgcgcac cggcgacccg gcgcgtccgc tgttcgtacc ctggagcgag
    14701 gacacgcgcg cgctacgaac atatccgcag cgaatggcgc gccatccgcg ccgaatggga
    14761 tggcgggcag cgccccagca gcgcgcaggc ggtggcgcgc ggcgacgcct tcgtgagcga
    14821 gatcgacgcc tttgtcgagg ccatcgaggt gcagatcatg cgctggacgg ccgggctgca
    14881 cctgtttcac ctgttcctgg tggcactcac catcgccgcg gccgtgacct tcatggcgct
    14941 cagctactgg ctggtggtca accccgtgaa ccagctgcgc caggcgctgg cggcgatgcg
    15001 ccgtggcgag ctgggcacgc gcctggaggt gcagaccgag gacgagttcg gccagctcac
    15061 cgccggcttc aacctgatgg cacatgcgct gcagacctcg cacgaggatc tggagggcaa
    15121 ggtgcgcgag aagaccgcca gcatcgagga gcagaaccag cgcctggccg cgctgtacgc
    15181 ggtgagcgct ctgaccggcg aggccggcag cctggaggtg ctggcccagg gctttgcgca
    15241 gcagatccgc agcgccgccg gggccgatgc cgccgccgtg cgctggtccg acgaggccaa
    15301 tgagcgttat gtgctgctgg cccatgacgg cctgccgagc accctggccg agaccgagca
    15361 ttgcctgctg gccggtgcct gcgaatgcgg ccagcccggg ccgcgcgcgc gcatgcgcgt
    15421 gatccccatc gtgccggcca gcggcgggca gctgcccgat tgcggccagg agggcttcca
    15481 gaccgtgatc aacgtgcctg tgcgcctgca ccagcgcctg ctgggcgagg tgacgctgct
    15541 gtaccgcggc gcgcgcaccc tgcccgatgg cctgcgcgac ctgctgggca ccatggccca
    15601 gcatctggcc acggccatgg agggcctgcg cgccagcgcc ctggagcgcg aggccgccgt
    15661 ggcgcaggag cgtgcgctga tcgcacgcga gctgcacgac tccatcgccc agtcactggc
    15721 cttcctgaag atacaggcgc agctgttgcg cgacgccatc gccaagggcc gcgacgccgc
    15781 gcgcgaccag agcctggccg agctggacgc cggggtgcgc gaatgctatg ccgatgtgcg
    15841 cgagctgctg gtgcactttc gcacgcgcac gcaggacgag gacatcgagg aggcgctgcg
    15901 cgtgaccctg tccaagttcg agcaccaaag cggcctgccg gccgagctga cgatggccgg
    15961 ccagggcctg ccgctggcgc ccgatgtgca gatccaggtg ctgcacatcg tgcaggaagc
    16021 cctgtccaac gtgcgcaagc atgccggtgc gcgctccgtg cagctggtgg tgcagcgcca
    16081 cccgcaatgg cgcttcgagg tgcgcgacga cggccagggc ttcgaccccg cggcggtgcc
    16141 gccggattcg ctgcatgtcg gcctgggcat catgcgcgag cgcgccgagc gcataggcgc
    16201 gcgcatcagc ctggacacgc aggccggcca gggcacctgc gtgacgctgg agctgccgcc
    16261 agcggggcgc gaggccccgg gcgctgcggc taccatggcg gccgtatgag cgcgcccatc
    16321 accctgttcc tgatcgacga ccacaccctg ctgcgccgcg gcctggtggc gctcttgggc
    16381 cagcatgccg acctgcgcgt ggtgggcgag gcgggcgacg ccggtcaggc gctgcgcctg
    16441 ctgccggtcc tgcgccccga cgtgatcttg ctggacaacc acctgcccgg cgtgcgcggc
    16501 gtggacgcca ttgccgacct gcgcgaggcc tcgcccgcga gccgcgtgat catgctcacg
    16561 gtgagcgagg acggcgagga cctggccagc gcgctgcgcc atggcgcgca gggctatctg
    16621 ctcaagacca tagacggcga cctgctggcc caggccgtgc gccgcgcggc cagcggcgag
    16681 ccggtggtca gcccggagat gatgggcaag ctggtggcgg ccttccagtc ccagggcgcg
    16741 cccgagcccg aacccgcgcc cgaggaggcg gagccggcgc cgcagctgtc gccgcgcgag
    16801 gaagaggtgc tgcgcgagat cgcgcgcggc gccagcaaca aggagatcgc gcgtgccctg
    16861 gacatcgccg agaccacggt gaagatccat gtgcagcaca tcctgcgcaa gctgggtctc
    16921 agctcgcgcg tgcaggcggc ggtctatgcc tcggatcgcc agcgtgccga gtagcaatct
    16981 ctttttgata gctgtcaacg cttgccacac aagggttcat tcctgttttc tgaaaactgc
    17041 ccctgttttc aaagggatgg ctgttgtacc gtcgatcagt gccggtagaa cgccaccaga
    17101 gccaggatca ccaggcacat cagcgcgttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnna tcagtgccgg tagaacgcca ccagagccag gatcaccagg cacatcagcg
    17281 cgttgccgat gttgaccacg atggcgcgat tcagccgccg cccgttgtcc tcgtagagcc
    17341 aggccgccat cgtcatgttc gagctggtcc agatcgccca agtgagcagc gaatgctgct
    17401 gtgaactgcc gcttgccagg atggcccagg ccgtgggcag gtagccgagt atgcgcaccg
    17461 agctgaacag cgtgaacgcc caggccagca ggcgcaggta gttttcccgc gccctgctgg
    17521 caccctccac gggacccgga tcgcctgccc gctggccctg cgcctggctc atggcacgct
    17581 caccaggaac accggggcaa agcccgcatc ggtcagccgg tcggccaggc gcgcggcctg
    17641 ctgcaggtcg ctgccccgca ccagcacggg catgtccgac ggcgcgtcca ggcttgcgtg
    17701 ctgggcccag gcggacacca tcgcatcgtc caagccccgg tcaccgcagc agccagcgaa
    17761 gacgctgatc gccccgccag gcaaggtctg ctcatgggcg cgggcctggg cctccgtgat
    17821 gtacagtccg gcgcgcgtgc ggtgggcagg acttgcggga atggcagcgg gcacattggc
    17881 cgctggccta ccttcatcat cgaacagcgg cgtggattgg ccggatggct gttgggcgca
    17941 ggcgcccatg caggcggcca tgccgagggt ggcgcagagc aggatgcggt tcatggtgac
    18001 tctccttgca agtgattggg gacatgcggc gacggcatgg ccgtcactcc tgggcgatgc
    18061 cgtagtcgcg cacgaaggtc gcccacgcgg tggaggcggc ctcgacggcc tcggcgagct
    18121 gctgtggtgt gctgcccagg ggctcggtgc cgctggcccg cagcggttcc tgcacggcgg
    18181 gggcttgcag cgcccgggcc accgccgccg acagcctggc ggccacctcg ggcggcgtgg
    18241 cggcgggggc gaacaaggca ttccaaaagg acagcgtgcc cgaggaaacg ccgagttcgg
    18301 cgaaggtcgg cacgtccggc agcagcgggc ttctttgcgg cagggccgtg gccagcaccc
    18361 gcagcttgcc cgccttggcg tgctgcaggc cgcccgcgat tgggccgaag ttgatctgca
    18421 cctggccgct gatgaggtcc ggcatcagtt gcgcgccgcc cttgtacggc acccgccggg
    18481 cctgcacgcc tgcggccttc agcacgcgct cggccagcat gtattcgccc agcgtgccgg
    18541 tggccaggtg aagctggcct ggatgggaac gcccgtaggc caccaggtcg gcgaagttct
    18601 gcaccggcag ctcgcggttg acgaacagcg cgaacccgag ttccacgacg ttggcgaccg
    18661 gcgtgaattc gctcaaggct ttgtagggaa cacccttctg caccagtggc agtcccgcca
    18721 tcgccgacag gccccagagc agggtgtagc cgtcgggcgg cgcgtccatg accgcctgcg
    18781 cggcgatggc gccacccgcg cccggacggt tcaccacgac gaccggctgg ccccaggcgg
    18841 cgctgagtga cgcggccacc agccgcgcgg cgctgtcgga tgcgccgccg gcaggcgtcg
    18901 gcacgacgat ggtgatggcg cggctcgggt aatcggcggc gcgcgccagc gggacgttgc
    18961 agagcgcggc cattgcggca aggcacaggg tgcggcgatc cagcgtcatg gcggattcct
    19021 cttcgtggtt gtcgatggcg tcatcgtcgc cacccagcgc gcccggcgca tcgtccagtg
    19081 gtcttattgg agtaccgggc ctggtgcttt ggtaccggta cttgcgcaca gtccccggca
    19141 cggccgtgga tggctaagat gaaccgccat gacgtccgcg caccaccttt cccccatggg
    19201 ccgccgcagg ttgatggccg cagccgcggg catcgcgctg ctgcccccac tgataccccc
    19261 tgtccaggcc caaccctcgc gcacggcccg catcggctgg ctgggctgga tcggcgcggc
    19321 gggagcggcg gcttccgcgc tggcgctggc ggcctttcgc gcggggctgg ccgatcgggg
    19381 gtgggccgag gggcgtggct acgaactgct ggtgcgcgac ggtgaccagc cgcgcagcgc
    19441 cgatctggct gcggagctgg tgcgatccgg cgtcgatctg ctcgtcgcgc aggggccgat
    19501 ggtcttcggt gccaggaagg tgtcgggcgc gacgcccgtg atcttcaaca tcggcggcga
    19561 ccccgtcgag gcggggctgg tcgccagtct gtcgcaccca ggcggccatc tgacgggcgt
    19621 cacctcgcta tcgaccgaac tggcgggcaa gcggctcgag ttgctgaaga cggccatgca
    19681 gtggggctcg cacgtggcgg cgatcgccaa cgccctgcac gcgggcttca ccatcgagcg
    19741 cgacgcggtg caggcggcgg cccggcggct cggcctcacg ctgtcctggc atctgctgaa
    19801 gtcgatggat gatttcgata ccgcgctgcc ccgcatcgcc agcgaaggtg cccacgcact
    19861 gctggcggtg cccgacaacc tcatcaacgt tcgggcgcaa acgctggcgc gcttctccgc
    19921 agctcaacga ctgcccagca tttcgggctg ggccgcgttc gccgaggcgg gcaacctgat
    19981 gagctacggc ccgaacgtgc atggttacta ccggcagatg gcggccatcg cagaccggct
    20041 gctgcgcgga gcgcgcgccg ccgacattgc cgtggagcaa ccgcgcgaca tcgagctggt
    20101 cgtcaacgcc aaggtggcgc gtgcgctggg cctggatctt cccgccgagt tgcagctccg
    20161 tgccgaccgc tggatcgaat gagcgccctt ggccgggctg gtggcgcagc gcgctggggc
    20221 ggcgctatgc ggcggtcttc gcgctgctgg tgtcgctgcc gctgctggcc tacgcggcct
    20281 ggagcgctgc gcaggtgtac cgccagcagc gcgacgcgct ggccgccgta cagactgcgc
    20341 aggccgatgc ggccgccgcg cgcatccagc ggttcctgcg cgaaatcgag ctgcaactga
    20401 gctggctgac cgccctgccc tggacgcccg acggcgcccc gcagcggcgg ctggatgcgc
    20461 tacgcgcgct gcggcagacg cctgccatcg ccgacatagc cctcatcgac ggacagggcc
    20521 gcgagcgggt ggcggaatca cggctggcgc tgggccgcgt ggattcgatg gccgaccgca
    20581 tggcctcgcc cgcgttctcg ggggcgcgcc aggcgcgcgg cgcgtatttc ggcgacgtca
    20641 gcttccgcaa gggcagcgag ccgttcatga ccgtggccgt cgcggggccg cgcggcgagg
    20701 cgggcgtggc catcgccgag gtgagcctga agtacatctg ggacgtggtg tcggagatcc
    20761 gcatcgggga cgaaggcatc gtctatgtcc tcgatccggc ggggcggctg atcgcccatc
    20821 cggacatcaa cctcgtgctg cgcaacacgg acttgtccgc cacgctgcgc gccttcgaga
    20881 accagaaagc cgctgccccg ccacgcccgg cattgcggct ggtgggcaca cggggcgaac
    20941 cggtgctggc cagcagcgtg atgctggccc cgccgggctg gcgggtcgtg gtcgaacaag
    21001 gcttgcaaga ggccgacgcc cccctgtggg ccaacgtgca gggtgcgctg tgggtggcgg
    21061 cggtgagcct gctgctggcg ctgtcggcgg cactgtggtc ggcctggcgc atcgcccggc
    21121 cgctgcgcga gctggcccag ggcgcagagc ggatcggcgc cgggcagttg tcgcaccgca
    21181 tcgccctgca ttccggcgat gaactggagc ggttgggaga gcgtttcaac agcatggccg
    21241 ctgagctgca ggccagccac gacaacctgg ggcggcaggt ggaagagcgc acgcgcgagc
    21301 tgtcggatgc gaaccgtgcc aagtcgcgcc tgctggccgc cgccagccac gacctgcgcc
    21361 agccactgca cgcgctgaac ctgatggtgg cgcaatggcg gcgtgacccc gatgccgccg
    21421 agcgccggcg cctggggctg cgtgtggagc aggccatcgc cgccatcaat gcgctgttcg
    21481 acggcctgct ggacgtgtcc aagctggacg cgggcgtcat ccgcccgcag gtggaggcgt
    21541 tcccggtaca gcgcctgctc gaccgcatgg acgtggcgca cgcggcggcc gcgcgggaca
    21601 aggggctgga gctgcgcatt cgcccttgcg acgcctgggt ggccagcgat ccagtgctgc
    21661 tcgagcgcat cctcggcaac ctggtcgcca acgcggtgcg ctacacgcgc agcggggcca
    21721 tgctgctggg ctgccggcgc acgcgggccg ggctgcgcat cgccgtgtgg gataccggga
    21781 tcggcatcgc gcccgacaag caggagcgca tcttcgatga gttttaccgg ctcgacgccg
    21841 aaggcggcgc ggccgatggc ggactggggc tgggcctgtc catcgtcgcg cggctggccg
    21901 gtttgcttgg ccaccaggtc agcgtgcgct cggttcccgg acggggttca tgcttcgcgg
    21961 tcacggtgcc cgaaaccgcc agccagccgc acctccccac ggctgcgaac gactccgcca
    22021 tcgatgcatt gcagggccgc cgggtgctgg tcatcgacaa caacgcgcag gtgctggcga
    22081 gcacggcgcg gctgctcgaa ggctgggggt gctgcgtgct ggctgcgcac ggcgcgccgg
    22141 atcacgtgca tggcccggtc gatctgctgc tggtggacat gcgcctggac cacggcgatg
    22201 acggcgttgc ggccatctcg cgcctgcgcc gccagtgggg gcgggatgtg cccgcgctca
    22261 tcctgacggg cgacaccgcc attggcacgc gcgagcgcat cgccgccagc ggcctgccca
    22321 tgctggaaaa gccggtttcg gcgttgcgcc tgcgctcggc gctgacgcgg ctgcttcagc
    22381 gcggttgagg caacgcccac cccaggcgcg tgacggccag tacggcctcg gtgcggctgt
    22441 ccacccccag cgcccgcagg atggcggaga cgtggttctt caccgtgggc tcggcgagcc
    22501 ccagggcacg gcagatcagc ttgttgttct tgccctgcat gagcagggcc agtacttcga
    22561 gttggcgccc ggtcaggccc agcgcctcgg gcgttgccgc tcgggtgtcg aacgtgcgcg
    22621 ctgctgtctg caaggccacc ggcggcacgt aaacgccacc cgccagcacc aagcccaatg
    22681 cgccggcaat gatttcgcga ggctcggtct tggggacgta gcccaccgcg cccgccgcca
    22741 gcgcctcttg caccgtctgg cgatccaggt cgccggagag catgacaacg gcggtgccga
    22801 tgctcgccgc cccgatcctg cgcagcaggt cgagcccgga gccatcgggc agccgcaagt
    22861 ccagcagcac cagatccacc gcctggccgc agcccagggc cgtgatcgcc tccgcgccgc
    22921 tcgccgcctg ctgcaccctc gagcccggcc tcaactccag caacaaggat gcgagcgcct
    22981 cgcgcaccag gggatggtca tcgacgacca ggaagttcat gggttggtgg agaagggctt
    23041 gggatcgggg catcttagca aactataaaa aatatagcta actgcgcaat gtagttgagc
    23101 gataaaggcc aaaacaccgt tggaaatcct ggggtgcgga ctagttcttt cggagtaagg
    23161 agccggcgcg gcccatcgtt cttttgccac ggcccatgcg ttccttcggg gtatgggaaa
    23221 acccggggtg ccggtggaac atccatgcac acagcaacca aggactaccg catggcaccc
    23281 gcgtcgcatt ccgcggcaca agacccccgc cgcaaccgcc aggcctggtc ggtgctcatc
    23341 gtcagcaccc tggccttcac cgtctgcttc atggtctgga tgatgttcgg catcatcggc
    23401 atcccgatca agaagatgct ggacttgaac gccacgcagt tcggtctgct gacggccatg
    23461 ccggtgctca cggggtcgct ggtgcgcgtg ccgctcggta tctggacgga caaatacggt
    23521 gggcgcatcg tcatggccgt gctgatggcc atcaccgtgc ccgccatctg ggtcatgggc
    23581 tacgccagcg agtattggca ctttttggtc ataggcctgt tcgtgggcct ggccggcggc
    23641 gcgttttcgg tgggcacgcc ctatgtggcg cgctggttcc ccagacaccg ccagggcacg
    23701 gccatgggcg tgtacggcgc cggcaactcg ggcgcggcgg tgaacaagtt cgtcgccccg
    23761 gtgatcctgg tggcctttgg ctgggcggcg gtgccccatg tctacgcggc catcatgctg
    23821 ggggcgctgc tgctgttctg gttcttcagc cacagcgacc ccagccacct ggtggccagc
    23881 aacatcagct tcaccgacca gctcaaggcg ctgaaagatc cgcgcgtgct caagtactgc
    23941 cagtactaca gcatcgtgtt cggcggctat gtggcgctca gcctgtggat ggtgcagtac
    24001 tacgtgggcg aatatggcct ggacatccgc gtcgccgcgc tgctggcggc ctgcttctcg
    24061 ctgcccggcg gcgtgctgcg ggcgattggc ggcgtcttga gcgaccggta cggcgcgcac
    24121 agcgtcacct ggtgggtgat gtgggtgagc tggatctgcc tgttcctgct gtcctacccg
    24181 cagaccgcct tcaccatcca gacggtgaac ggccccagca ccttccacat cggcctgaac
    24241 gtctatgtgt tcaccgcggt catggccgtg cttggcgtgg cctgggcctt cggcaaggcc
    24301 agcgtgttca agtacatcag tgacgactac cccggcaaca tcggcgccat cagcggcatc
    24361 gtgggtcttg ccggcggcat gggcggcttc atcctgccga tcatgtttgg cgcgctcatg
    24421 gactggacgg gcatccgctc cagcgccttc atgctgatgt atggcgtggt ctgggtgtcg
    24481 ctgatctgga tgtactggac cgaggtgcgc cgcaccgatg tgatgaccgg ccagggtgtg
    24541 gcggccgcca cgcccgccgc ccgctgatcg accggagaac ccacaccatg tctgccgata
    24601 ccgttgcaca agagggcgcg cgccaggggc ggctgctgca catctggacg cccgaggaca
    24661 agcagttctg ggagcgcgag ggcgaggctg tggccaagct caatctgtgg atctcggtgc
    24721 ccgcgctgtt cctggcgttt gccgtctggc aggtctggag cgtggtcgcc gtgaacctgc
    24781 cggcgctggg cttcaagtac tcgaccaacc agctgttctg gctggccgcg gcgccggcgc
    24841 tgtcgggcgc cacgctgcgt atcttctact ccttcatggt gccggtgttc ggcgggcggc
    24901 gctggacggc gctgtccacg gccacgctgc tcattcccat gctgggcata ggctttgcca
    24961 tacgggacaa caccaccggc tacccgacca tgctcatgct ggcgctcttg tgtggcctgg
    25021 gcgggggcaa cttcagctcc agcatggcca acatcagctt cttcttcccc aaggagcgca
    25081 agggctcggc gctgggcgtg aacgcgggcc tgggcaacct gggcgtgtcg gtcgtccagt
    25141 tcctgagccc gctggtgatc tcgctgggca tcttcggcat ctttggcggc gacgcgcagg
    25201 tcatcgtgag gaacggcgag cagcagcatg tctgggcgca gaacgcggcc ttcatctggg
    25261 tgccgtggat cgccatcacc gccgtcgtgg cctggttcgg catgcacgac atcgccgatg
    25321 cgaaggccag ctttgccgcc caggcggcca tcttccgcgc caagcacaac tggatcatgt
    25381 gcctgctgta tctgggcacc ttcggctcct tcatcggctt tgccgcgggc tttccgctgc
    25441 tgatgaaggc gctgttcccg gccatcaacc cgctggccta cgcctggctg gggccgctgg
    25501 tgggggcgct cacgcggccg tttggcggct ggctggcgga caagatcggt ggcggcgtgg
    25561 tcaccttctg gaacttcatc gtcatggcgc tggcggtgct gggcgtgctg ttcttcctgc
    25621 ccaagggcgc gagcgcctat gccctgccgt tcggcccggc cgagggcagc ttcaccggct
    25681 tcttcctgat gttcctggtg ctgttctgca ccaccggcat aggcaatggc tcgaccttcc
    25741 gcatgatccc ggtgatcttt ctgacgcaaa ggctgcgcgc gctgcgcggc aacgacgagg
    25801 ccgcccaggc ccaggccatc aaggacggca acaccgaggg cgcggccgcc gtcggctttg
    25861 ccggggcgct tggcgcctac ggcggcttct tcatccccaa gagctatggc agctccatcg
    25921 ccagcaccgg cggaccggag ttcgcgctgt ggatgtttgt cgtgttctac agcctgtgcg
    25981 tcgtcgtcac ctggtggtac tacgcacgca ggaacgccga gatgccttgc tgacaagagg
    26041 ttgagcgttg agggttgagc gttttgcgca aacccaa
//
LOCUS       DAOMTQ010000025        26048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_192_length_26048_cov_25.299811, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000025 DAOMTQ010000000
VERSION     DAOMTQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 26048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26048
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192_length_26048_cov_25.299811"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="PK518_04225"
     CDS             complement(<1..1526)
                     /locus_tag="PK518_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRM48148.1"
                     /translation="MTWALLGGVAGVALQLQQAALWPWSAYAALLGAGLLAAAGLAWP
                     AWVPGRRQGMALPLVAMALAFGLCGLRATVFLDHALQPALEGRDLQVTGVIAAMPQAR
                     ETGLRLRLAVESARSDGAPVRLPPLVDLAWYAGAAGDAAQADPPPAVHAGERWRLTVR
                     LKAPHGARNPHGFDYELWLWEQGVQATGYVRSSARLDALHGPPERLAATWRYPVEQLR
                     ERVRDAILRGVDSAADGAQQRAIGVVAALVTGDQRAIERADWDVFRATGVAHLMSISG
                     LHITLFAWLAAALLGRAWRRSARLCLMLPAPTAALVGGVLLAGAYALFSGWGVPAQRT
                     VVMLATVALLRLSGRRWPWPQVWLLACAVVLLADPWALLQPGFWLSFVAVAVLFATDN
                     RAVSAYPAGVTGHFYALLREQWVVTLALTPLSLLLFGQVSLVGLLANLLAIPWATLVV
                     TPLALLGVLWAPLWQAAAWCVQALTLLLQWLAAWPWAQIALPMAPLWAGVAAVAGGAL
                     L"
     gene            1668..2126
                     /locus_tag="PK518_04230"
     CDS             1668..2126
                     /locus_tag="PK518_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRM48149.1"
                     /translation="MSVYDKLKALNITLPPVAVPAAAYVPYVQTGNLVFLSGHIARKD
                     GKPWVGQLGKNMATEEGKAAARAIAIELMGTLHAAAGGDLNRVKRIVKLMSLVNSTGD
                     YTEQHLVTNGASELIGEVFGDAGKHARSAFGVAQIPMGACVEIELIAELN"
     gene            complement(2189..2476)
                     /locus_tag="PK518_04235"
     CDS             complement(2189..2476)
                     /locus_tag="PK518_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48150.1"
                     /translation="MTTTPSNDTTLALKLPEGAVFEDLKLRRCAQDAIDLDMDLVERI
                     CQLNGWDFAKVQANPGPVVSSILSVWYKQHLAEGGQPDATMEALKKPPTAH"
     gene            complement(2494..4842)
                     /gene="parC"
                     /locus_tag="PK518_04240"
     CDS             complement(2494..4842)
                     /gene="parC"
                     /locus_tag="PK518_04240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRM48151.1"
                     /translation="MTEQANTLLDPTAPADDSLGGYAQRAYLEYALSVVKGRALPDVC
                     DGMKPVQRRILYAMERMGLSWSGPNGNTPPKPVKSARVVGDVLGRFHPHGDQSAYDAL
                     VRLAQDFNQRYPLIDGQGNFGSRDGDGAAAMRYTEARLARITSLLLSEIDMGTVDFQP
                     NYDGSTQEPCQLPARLPFALLNGASGIAVGLATEIPSHNLREVADACIALVKNPNLAD
                     AELFGLIPGPDYPGGGQIISSAADIQDAYQSGRGSLKVRARWKIEDLARGQWQLVVTE
                     LPPGVSTQKVLEEIEDISNPKVKTGKKALTQEQQQLKASMLALLDGVRDESSKDAPVR
                     LVIEPKTGRVPQQDLITALLAHTSLETSAPINLTMVGIDGRPVQKSLRQMLVEWIAFR
                     QQTITRRSQHRLAKVLDRIHILEGRQLVLLNIDEVIAIIRAADEPKAALIARFNLSDR
                     QAEDILEIRLRQLARLEAIKIEQELKQLREEQGKLEDILANPASLRRLMVKEIEGDAK
                     QFQDARRTLIQAEKKAVAEVKIVDEPVTVVVSDKGWVRARTGHGHEAGAFAFKAGDAL
                     YATFECRSVDQLIVFGSNGRVYSVTVASLPGGRGDGQPITTLIELETGTQIRHYFAGP
                     LAAQLLLSGSGGYGFLAQVEHMVARNRGGKAFVTLGEGETLCVPSHAAGSSASAPLAA
                     ATHVCCASVGGRILTFEISELKTMAGGGRGLQLIALEDKDSLAGAAAYTRSVRIAGLG
                     RGGKEREEQLEIRSLNNARAPRGRKGKAADLGFKPATVTRVE"
     gene            complement(4909..5826)
                     /locus_tag="PK518_04245"
     CDS             complement(4909..5826)
                     /locus_tag="PK518_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRM48152.1"
                     /translation="MRVKKPETPPEPNVPALYATLLADIKQRVRRAQVRAMLAVNAEL
                     IRLYWDIGRVIDERQQQEGWGAGVIPRLARDLHNDLPEEKGFSERNIKQMLAFYREYA
                     QLSFVQQPAAQIETEPKVPQAAALFTAELILALPWTHHAILMAKVKDPATRHWYMQAA
                     LEHGWSRSILVMQIETAAHQRQGRAASNFALRLPKPESDLVQQTLKDPYVVIELKRGE
                     FKPEYAGKMNFYCSVVDDRLRHAHDQPTMGLILCQQHNRVLAEYALRGVEKPIGVSSF
                     ELTRALPATLESSLPSIEQIEQELGGIAE"
     gene            complement(5817..>8092)
                     /locus_tag="PK518_04250"
     CDS             complement(5817..>8092)
                     /locus_tag="PK518_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HRM48153.1"
                     /translation="DTTTTVIVATRQAFQVEEEECRKVYQSSGALMHHFDHLAPAQRA
                     ELLADGEGTERSTPYSLANVLRLRRPFVVVDEAHNSRTELAFDMLARFRPSGVMELTA
                     TPDVQRTPSNVLHSVSAAELKAEEMIKLPVLLQTEPHWQQCLADAIGRRDALQALADE
                     ERRAGAPYLRPLVLIQSEPRRAGVETLDFDKLRQELIANHGIPASEIVVATGEEKGLE
                     QIDADYPLGMADPACPVKFVITQKALAEGWDCPFAYILVSMASMSSSTAVEQLLGRVL
                     RQPGASQRASPLLNQSYAFVVSRDFAETAGALRDRLVAGAGFERREVAEFVAAVLPDQ
                     ARLDLEGHAGRVVLRPVAVALPEKPDLKSVPKTVRGKVSWDGQLNTLTISAPLTEDEA
                     ETLKACVSSPAAAAAIVQAAEVSRTTAIEFFQTPAELGERLRVPQLALRVPVQQGELV
                     LFDDPEVLDYPWDVSLYDAAPTAADLSALGAALKVSEGGAIDIDEGSGKVFTRFLPEL
                     QRDLGLVYQPENWDAVRIATWLCRNLPEPSLTHPSKQAFVAAWLSDLLRRDGYTLARA
                     NLQKFQIRNLIEARIRELRKQAVGKAFQQALFGDDAAMRVAVSDQYAFEFAPHAYAPS
                     RDYDGRFGQFDFRKHFYGRMGDFDSKEEFECACQLDMWAQQGRIQFWVRNLVRREGCS
                     FFLQKADGRFYPDFVCQLPNGTVLVVEYKGADRWSSAEDDRLIGNLWAELSGGRCRFV
                     MVKDRQWDGVRELLPCA"
     assembly_gap    8093..8192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8361..8585)
                     /locus_tag="PK518_04255"
     CDS             complement(8361..8585)
                     /locus_tag="PK518_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48154.1"
                     /translation="MNGKPLENARSSDLRGSWQALQRAAQRAREVAVQTGTDLVVTRN
                     GVLEHIKPQPTPTPTPTPELDSPETKAESA"
     gene            complement(8582..9106)
                     /locus_tag="PK518_04260"
     CDS             complement(8582..9106)
                     /locus_tag="PK518_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HRM48155.1"
                     /translation="MAAQERKVIIIAGPNGAGKTTFAREFLPHEAACPVFVNADLIAA
                     GLAPFAPESAAVQAGRLMLSELERHFQAGSSFAFETTLSGRAYLRHIARWQQAGYEVK
                     LIFLRLSSAEEAVARVAQRVRQGGHDIPPEVIRRRFVAGLENFQRYYAPAVDVWALYD
                     NAGEVPVLLDWSER"
     gene            complement(9110..10720)
                     /locus_tag="PK518_04265"
     CDS             complement(9110..10720)
                     /locus_tag="PK518_04265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRM48156.1"
                     /translation="MPTLNWIGKDAVVKHHKDVPFRLLEPDAALSCAAADTGNLIVQG
                     DNLHALKALLPRYAGQVKCIYIDPPYNTGNEGWVYNDNVNSPEIRRWLGEVVGKEGET
                     LDRHDRWLCMMYPRLVLLKQFLREDGAIFVSIDDNEVGTLRLLMDEIFGSGNFVANVI
                     WQKKYAVANDHKTIAPMHDFVLVYQRSPSWQRNLLPRGEEKDSQYKFEDEKGQFRSSD
                     YTCAKTAEERPNLYYPVTNPNTGEEIWPKRTRVWSYSQDEHQRHRAEDLIYWGKDGKG
                     KVPAYKRYKHALRNDGVVPQTLWPHEFAGHTDGSRKELREVLHDVPSVSDFATPKPSL
                     LIQRILQIATDKDSIVLDSFAGSGTTAHAVLKQNAEDGGQRHFILVEMDENIAQNVTA
                     ERVRRVAGGYTNAKGQAAAGLGGGFQFCRLSSEPLFDAYGQIRSDVTFAELAKFVWLA
                     ETQTGYTGQADSPLLGVHEGRAIYLLYNGILKDKSVAGGNVLTAPVLAVLPPFDGPKV
                     IYAAANRLGSRTAREGITFKQTPYALEV"
     gene            complement(10817..11146)
                     /locus_tag="PK518_04270"
     CDS             complement(10817..11146)
                     /locus_tag="PK518_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HRM48157.1"
                     /translation="MPQFDVHLNPGPQRGTVPLVVLVQSALFDGYRRRVVVPLVLRSA
                     LPAGGRVAGTRMNPVFEIAGQTVVLHPLDMVSVALEQLGPVVGSLAEQGQAISDALDE
                     LLTRSWG"
     gene            complement(11158..11403)
                     /locus_tag="PK518_04275"
     CDS             complement(11158..11403)
                     /locus_tag="PK518_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HRM48158.1"
                     /translation="MTALESSAKKPVNLSLNEALVRESRAYCGNLSAKVEEMLQAYVE
                     AQRQARSQHQQQAQAAVADWNALHDASGSFADAHSTL"
     gene            complement(11574..11837)
                     /locus_tag="PK518_04280"
     CDS             complement(11574..11837)
                     /locus_tag="PK518_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRM48159.1"
                     /translation="MNKPTKTIPHFASEAEERAFWESQDSTDYLDWSQAHSVVLPNLK
                     PTTKTISLRLPQHLLDSIKAAANARDVPYQSLIKVWLQEKLHR"
     gene            complement(11821..12120)
                     /locus_tag="PK518_04285"
     CDS             complement(11821..12120)
                     /locus_tag="PK518_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRM48160.1"
                     /translation="MLKLAEITGFDWDAGNERKNDKHGVSMAQAEQVFFNAPLLILGD
                     AKHSQQEPRYHALGRSDDGRLLHLTFTLRGSGSLIRVISARDMHRKERAFYEQAH"
     gene            complement(12166..14142)
                     /locus_tag="PK518_04290"
     CDS             complement(12166..14142)
                     /locus_tag="PK518_04290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRM48161.1"
                     /translation="MAVKPITSPAYSEGSIRVLKGLEPVKQRPGMYTRTDNPLHIIQE
                     VLDNAADEALAGYGKKIKVTLHADGSVSVEDDGRGIPFGLHPEEGAPVVELVFTRLHA
                     GGKFDKGVGGAYSFSGGLHGVGVSVTNALSTRLEVATHREGQIARLAFAAGDVVEPLV
                     ARPLETGERKQGTTVRAWPDAKYFESAQLPMGELTHLLRSKAVLMPGVTVQLINEKTR
                     DTQTWQYKGGLVDYLTQTLAADPVIPLFEGEGFASGSDESFAEGEGAAWAVAFTEDGA
                     PVRESYVNLIPTSAGGTHDSGLRDGLFQAVKGFIELHSLLPKGVKLMPEDVFARASYV
                     LSAKVLDPQFQGQIKERLNSRDAVRLVSSFVRPALELWLNQHVDWGKKLAELAIKAAQ
                     TRQRAGQKVEKKKGSGVAVLPGKLTDCESRDLAYNEVFLVEGDSAGGSAKMGRDKETQ
                     AILPLRGKVLNTWEVERDLLFKNNEVHDIAVAIGVDPHGPHDQPDLSGLRYGKVCILS
                     DADVDGAHIQVLLLTLFFRHFPKLIEAGHIYVARPPLYRVDVPARGKKPAAKLYALDE
                     GELEAILDKAAKDGVAREKCQISRFKGLGEMNAEQLWDTTLNPDTRRLLPVQLGAMDF
                     AATEGLITRLMGKGEAASRRELMELHGDAVEVDV"
     gene            14237..15454
                     /locus_tag="PK518_04295"
     CDS             14237..15454
                     /locus_tag="PK518_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM48162.1"
                     /translation="MHDATRKLSIAQVLICGATIVTLSMGIRHGFGMWLAPITQDMGW
                     TRQTFALAMAIQNLSWGIFGIFGGMMADRFGAFRVLILGALLYALGLAGMALSPTPLL
                     FSLTTGVLIGAAQAGTTYAVIYGVLGRQISAERRSWAMGVAAAAGSFGQFLMMPLEGQ
                     LIAQLGWQTALLVLAAMVLLIVPLAFGLREPGFHGSAPIQRTQSAGHAFAEALRYKSF
                     LLLTAGYFVCGFQVVFIGVHMPSYLKDHGLSPQVASTALALIGLFNVFGTYAAGTLGQ
                     RMAKRHILAFIYFARAVVITIFLLVPLSPMSVYVFSAVMGTLWLSTVPPTNAAVAQIF
                     GVQHLSMLSGFVFFSHQIGSFFGVWLGGYLYDTSGSYDIVWYLAIALGVAAGLINLPV
                     REGAIARPTPQAA"
     gene            15457..15585
                     /locus_tag="PK518_04300"
     CDS             15457..15585
                     /locus_tag="PK518_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48163.1"
                     /translation="MRATHKKWLAAAAAVLVLLAVFALYLRPDFMLTLADQIWACF"
     gene            15585..16136
                     /locus_tag="PK518_04305"
     CDS             15585..16136
                     /locus_tag="PK518_04305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM48164.1"
                     /translation="MHGLAVPSDWIRRWSHLVPAGGSVLDVACGQGRHMRWFLERNHP
                     VTGIDKAQEAIESIAYLGEAICADIEAGPWPLPGRRFDCVVVTNYLWRPLLPTILDSV
                     APGGVLLYETFAQGNESVGRPARADFLLRPGELLQAFGALRVVAYEDGFLDAPARFVQ
                     RIAAVREPLVPSGPLRLPLVGTP"
     gene            16171..17082
                     /gene="dapA"
                     /locus_tag="PK518_04310"
     CDS             16171..17082
                     /gene="dapA"
                     /locus_tag="PK518_04310"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRM48165.1"
                     /translation="MTSSSATLTGSIVALVTPMHDDGSVDYPTLRKLIDWHIQEGTDC
                     IGVVGTTGESPTVNVEEHCEIIRVSVEQSAGRVPVMAGCGANSTFEAIELARYAKKVG
                     ANSQLQVVPYYNKPTQEGQYQHFKAIAEATGDLPMVLYNVPGRSVADMHHETVLRLTQ
                     VPGIVGIKEATGNIERAQWLIRDVPKGFAVYSGDDPTAVALMLCGGQGNISVTANVAP
                     RLMHELCVAAIAGDRRRAMDIQFQLLPVHKNLFVEANPIPVKWAMQRMGLCGGAMRLP
                     MTPLAQGNEAVVETALRAAGLLAADRG"
     gene            17146..18234
                     /gene="bamC"
                     /locus_tag="PK518_04315"
     CDS             17146..18234
                     /gene="bamC"
                     /locus_tag="PK518_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HRM48166.1"
                     /translation="MNATTRLGLLGLAMTLSACSVLENDKIDYKSATKGATLEVPPDL
                     NQLSRETRYTVPGATVSAAAFEAGQARQPRGTASAAPQAIGDVRIERDGNQRWLVVNR
                     PADQLWEPVRDFWLESGFVLTVEQPKLGLLETDWAENRAKLPQDIIRSTIGKVFDSLY
                     STGERDKFRTRLERTAGGATEIFITHRGMEEVYTSSQKDNTIWQPRPANAELETEFLR
                     RLMVKLGVSEEQSKAVAAAPVPVTPSARMATLNNVPVVQLDEGFDRAWRRVGVSLDRT
                     GFTVEDRDRSQGVYFVRYVAPGEKKEQGFFSKLFSSSPDAVAPLKYRIVVRSEGNQST
                     VSVLNATGAPETSANAQRIVRVLADDLK"
     gene            complement(18322..18660)
                     /locus_tag="PK518_04320"
     CDS             complement(18322..18660)
                     /locus_tag="PK518_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48167.1"
                     /translation="MKNSVVLAALIAAAALAACGKKEEPAPAPAPAPVAAPAAEAADA
                     AKDAAAAAVDATKDAADATKDAAAAAVDATKDAAAAAVDATKEAADAAADAAKKAADA
                     AANAAAGASK"
     gene            complement(18858..19376)
                     /locus_tag="PK518_04325"
     CDS             complement(18858..19376)
                     /locus_tag="PK518_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48168.1"
                     /translation="MHADPNQEQATPALPSGRFEGREAFQKLVRDALACAAQEGWREL
                     IVSDANFHDWPLGERAVVESLQQWASSGRRLTMLAADYAEVQRRHARFVQWRVRWDHI
                     IVCRRAGAVDAQDLPSAIWSPQWVLQRHDPLRCNGVTGAEPERRILLREAINEWLERK
                     SAPGFPASTLGL"
     gene            complement(19366..20481)
                     /locus_tag="PK518_04330"
     CDS             complement(19366..20481)
                     /locus_tag="PK518_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM48169.1"
                     /translation="MDVQQPLALLGGLSPAQFMRRHWHKKPLLVRQAIAGFKPLLARA
                     ELLELAGREGVESRFIQDKGGQWVLRHGPLARRSLPSLATPRWTALVQGVDLHHEGVH
                     ALMQQFRFVPEARLDDLMISYATDGGGVGPHFDNYDVFLLQAHGKRRWRIGRQKDKTL
                     RPGLPLKILAAFEAEEEHVLEPGDMLYLPPGWAHDGIAVGECMTYSIGFRSPNGAQLA
                     HEVLQRMAEGAADEEGAIYRDAQQPAVAGPGAIPAELQDFARQAVQRALAQPQALECA
                     LGEALTEPKPNVWFEPAEAGGMLECLTLDRRTRMMYDDKHVFINGESYRASGRDATLM
                     RRLADERRLEPRDLVRASDEALELLSAWCEAGWAHAR"
     gene            20535..21059
                     /locus_tag="PK518_04335"
     CDS             20535..21059
                     /locus_tag="PK518_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM48170.1"
                     /translation="MEITEQCVVALTWVLKDTLGEELDVLDDPVEFLVGGDDLLARIE
                     EALQGHCVGAALALHLEPEDAFGDYQDKLLFLEPRHLFPDDIEEGMTFEGAALPEGIN
                     PEAPRDALYTVAQIYPEHVVLDGNHPLAGIALRLALKVHGVREATEEEIGRGTAGTGF
                     FRIQPQAPGNDLLH"
     gene            complement(21076..21522)
                     /gene="dut"
                     /locus_tag="PK518_04340"
     CDS             complement(21076..21522)
                     /gene="dut"
                     /locus_tag="PK518_04340"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRM48171.1"
                     /translation="MKIDVKILDPRMAEQLPTYATPGSAGLDLRACLDAPLELAPNAW
                     QLVPTGIAIYLKDPGYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSAWNRSD
                     QAFTLQPMERLAQLVIVPVLQAQFNVVTEFPASERGEGGYGSTGKH"
     gene            complement(21541..21933)
                     /locus_tag="PK518_04345"
     CDS             complement(21541..21933)
                     /locus_tag="PK518_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48172.1"
                     /translation="MSAPQPWLGDYGEPPGGDYARYIERLLQSQDLRRGVLQAGAAAG
                     QRPVAVAKAAAPAAATTAPRRPAKQGANQAVTRTLSAKWPLLLGALLVLLMWFAPALL
                     PFVMAGVIVAGVGHGVWKSRHGRGGRDR"
     gene            complement(21930..23162)
                     /gene="coaBC"
                     /locus_tag="PK518_04350"
     CDS             complement(21930..23162)
                     /gene="coaBC"
                     /locus_tag="PK518_04350"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRM48173.1"
                     /translation="MNELSGKHIVLGLSGGVACYKSAELTRLLTKAGATVQVVMTEAA
                     EQFITPVTMQALSGRSVYRSQWDAREANNMPHINLSREADAILIAPCSADFIARLVQG
                     RADELLSLMCLARPMERVPLLIAPAMNREMWAHPATRRNLAQLAQDGATVLGVGCGEQ
                     ACGEVGDGRMLEAEEILEELQAFFAPKLLVGQRVLITAGPTFEAIDPVRGITNLSSGK
                     MGFAIARAARAAGAEVTLVAGPVHLPTPRGVRRIDVRSAQNMLQAVDTQAPEATIFIA
                     TAAVADWRPAHAAEHKIKKDGSGDTPTLAFVENPDILAQVAQSGRARAGQLYCVGFAA
                     ESENLLAHASAKRVRKGVPLLVGNIGPATFGQDDNALLLVDAQGHRELPRAPKSELGR
                     QLVAEIARRLGEAGAPRS"
     gene            23265..24911
                     /locus_tag="PK518_04355"
     CDS             23265..24911
                     /locus_tag="PK518_04355"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRM48174.1"
                     /translation="MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIETRMKQANNFTTGRIYQSVLEKE
                     RRGDYLGKTVQVIPHVTGEIQEFIKRGAGIGTADAVDVAICEVGGTVGDIESLPFLEA
                     VRQLSLKLGPNNSAFVHLTYLPWIATAGELKTKPTQHTVQKLREIGIQPDALLCRAHH
                     AVPDEEREKISLFTNVQEWGVISMWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPAN
                     LKRWDDLVREYEHPQGEVQIAMVGKYVELSDAYKSVNEALKHAGMQSHMRVKITHVDS
                     ETITDANAAEQLGQYHAILVPGGFGSRGVEGKISTARYAREHKVPYLGICLGMQVATI
                     EYARHVAGLDGANSTEFDAHCQHPVIALITEWKDEDGSIKTRDENSDLGGTMRLGAQS
                     SDVQAGTLAHSIYGSVVTERHRHRYEANVQYLDQLRKAGLVISALTQREHLTEIVELP
                     QSVHPWYIGVQFHPEFKSTPWNGHPLFNAFVKAAIAHQKA"
     gene            24927..25784
                     /gene="kdsA"
                     /locus_tag="PK518_04360"
     CDS             24927..25784
                     /gene="kdsA"
                     /locus_tag="PK518_04360"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRM48175.1"
                     /translation="MQLCGFDVGLDQRFFLIAGTCSIESLQLSLDVAGQLKEACTALG
                     VPLIYKGSFDKANRSSGSSQRGVGIDAGLKILAEVRRQLQLPILTDVHEAAQVAEVAS
                     VVDVLQTPAFLCRQTDFIRAVAQSGKPVNIKKGQFLAPWDMKNVIDKARDAAREVGLS
                     EDRFLACERGVSFGYNNLVADMTSLAEMRNSGAPVVFDVTHSVQKPGGLGAVSGGARA
                     MVPVLARAAVGVGVAGLFMETHPRPAEAWSDGPNAVPLRHMRALLETLVALDQVTKKN
                     GFLEDHFDA"
BASE COUNT         4422 a         8749 c         8353 g         4424 t
ORIGIN      
        1 agcagcgcgc ccccggccac ggccgccacg ccggcccaca gcggcgccat gggcagggcg
       61 atctgcgccc agggccaggc ggcgagccac tgcagcagca gcgtgagcgc ctgcacgcac
      121 caggccgccg cctgccacag cggcgcccac aacacaccca gcaacgccag cggcgtgacc
      181 accagcgtcg cccacggaat ggccagcaga ttggccagca gcccgacgag cgacacctgg
      241 ccaaacagga gcagcgagag cggcgtgagc gccagcgtca ccacccattg ctcgcgcagc
      301 aaggcataaa aatggcctgt aacgcccgct ggataagcgc taacagctct attatcagta
      361 gcaaataaca ccgccacggc aacgaagctg agccagaacc ccggctgcag cagcgcccag
      421 gggtcggcca gcagcaccac ggcgcaggcc agcagccaga cctgcggcca gggccagcgc
      481 cgccccgaca ggcgcagcag cgccaccgtg gccagcatga ccaccgtgcg ctgcgccggc
      541 acgccccagc cgctgaagag cgcataggcg ccggccagca gcaccccgcc caccagcgcg
      601 gccgttggcg cgggcaacat caggcacagc cgggccgagc ggcgccaggc gcgccccagc
      661 agcgcggccg ccagccaggc aaacagcgtg atgtgcaggc ccgagatgct catcagatgc
      721 gccacgcccg tggcgcgaaa gacatcccag tcggcgcgct ctatggcgcg ctggtcgccc
      781 gtgaccaggg ccgccaccac accgatggca cgctgctgag caccgtccgc agcgctgtcc
      841 acaccgcgca ggatggcgtc gcgcacccgc tcgcgcagct gctcaaccgg ataacgccag
      901 gtggcggcca gccgctctgg cgggccgtgc agcgcatcca gccgcgcgct gctgcgcaca
      961 tagccggtgg cctgcacgcc ctgctcccac agccacagct catagtcaaa gccatgtgga
     1021 ttgcgcgcgc catgcggggc cttgagccgc accgtcaggc gccagcgctc gcccgcatgc
     1081 acggcgggcg gggggtctgc ctgcgcggca tcgcccgctg cgccggcata ccaggccaga
     1141 tccaccagcg ggggcaggcg tacgggggcg ccatcgctgc gcgccgactc cacggccagc
     1201 cgcaggcgca agccggtctc gcgcgcctgc ggcatggcgg caatcacgcc ggtgacctgc
     1261 aggtcgcgcc cctccagagc gggttgcagc gcatgatcca gaaagaccgt ggcgcgcagc
     1321 ccgcacaggc cgaaagccaa ggccatggcc accagcggca gcgccatgcc ctgccggcgc
     1381 cccggcaccc atgctggcca ggccaggccg gccgcagcca gcaggccggc gcccagcagc
     1441 gccgcatacg ccgaccaggg ccacagcgcc gcctgctgca gctgcagcgc cacgcccgca
     1501 acgcccccca gcagcgccca ggtcatgcgg ccggcgccga ccctgggcca cagggccggc
     1561 tcgtcgatgg cagacgattc ctgctgcaac acggaggccg gtgcgctcat gggcgcatgc
     1621 taaggtacgc caccgatttt ttcattcatc tgttttcgag gactgccatg agcgtttacg
     1681 acaagctgaa agcactcaac atcaccctgc ccccggtggc cgtgcccgcc gccgcctatg
     1741 tgccctatgt gcagaccggc aacctggtgt tcctgtcggg ccacatcgcg cgcaaggacg
     1801 gcaagccctg ggtgggccag ctgggcaaga acatggccac cgaagaaggc aaggccgcgg
     1861 cacgcgccat tgccatcgaa ctcatgggca cgctgcacgc ggccgccggc ggcgacctga
     1921 accgcgtcaa gcgcatcgtc aagctgatga gcctggtcaa ttcgaccggc gactacaccg
     1981 agcagcacct ggtgaccaac ggcgccagcg agctcatcgg cgaggttttt ggcgacgccg
     2041 gcaagcacgc gcgcagcgcc tttggcgtgg cgcagatccc catgggcgcc tgcgtcgaga
     2101 tcgagctgat cgccgagctg aactaagcca actctacgta aaaggggcgc cgcgcggcgc
     2161 ccctttgcgt tgcagccagc cggccgcctc agtgcgccgt cggcggtttt ttcagcgcct
     2221 ccatggtcgc atcgggctgg ccaccttcgg ccagatgctg cttgtaccag acgctcagaa
     2281 tgctgctgac caccgggccg gggttggcct gcaccttggc aaagtcccag ccgttgagct
     2341 ggcagatgcg ctcgaccagg tccatgtcca ggtcgatggc gtcctgcgcg caacggcgca
     2401 gcttgaggtc ttcaaacacc gcgccctcgg gcagcttgag cgccagggtg gtgtcgttgc
     2461 ttggggtcgt cgtcatacca attccttgtt gcgttattcc acgcgcgtca cggtcgccgg
     2521 cttgaagccc aggtcggccg ccttgccctt gcggccgcgc ggcgcgcggg cgttgttcag
     2581 gctgcgaatc tccagctgct cctcgcgctc cttgccgcca cggcccaggc cggcgatgcg
     2641 cacgctgcgc gtgtacgccg ccgcgccggc caggctgtcc ttgtcttcca gggcaatcag
     2701 ctgcaggccg cgcccgccgc ccgccatggt tttgagctcg ctgatctcaa acgtcaggat
     2761 gcgcccgccc accgaggcgc agcacacgtg ggtggcagcg gccaggggcg cgctggcgct
     2821 gctgcccgct gcgtgcgaag gaacgcacag cgtctcaccc tcgcccagcg tgacaaaggc
     2881 cttgccgcca cggttgcgcg ccaccatgtg ctcgacctgc gccaagaagc cgtagccacc
     2941 cgagcccgac agcagcagct gcgcggccag cggcccggca aagtagtggc gtatctgcgt
     3001 gcccgtctcc agctcgatca gcgtggtaat cggctggccg tcgccgcgcc cgcccggcag
     3061 gctggccacc gtcaccgagt acacgcggcc gttgctgcca aagacgatga gctggtcgac
     3121 gctgcggcat tcaaaggtgg cgtagagcgc atcgcccgcc ttgaaggcga aggcgcccgc
     3181 ctcgtgcccg tggccggtgc gcgcacgcac ccagcctttg tctgagacga ccacggtgac
     3241 gggttcatcg acgatcttga cctcggccac ggcctttttc tcggcctgga tgagggtgcg
     3301 gcgtgcgtcc tgaaactgct tggcgtcgcc ctcgatctcc ttgaccatca ggcggcgcag
     3361 cgaggccggg ttggccagga tgtcctcgag cttgccctgc tcctcgcgca gctgcttcaa
     3421 ttcctgctcg atcttgatgg cctccagcct tgccagctgg cgcaggcgga tttccaaaat
     3481 atcttcggcc tggcgatccg acagattgaa gcgcgcgatg agcgccgcct tgggctcgtc
     3541 ggcggcgcgg atgatggcaa tcacctcgtc gatattgagc agcaccagct gccgcccttc
     3601 cagaatatgg atgcggtcga gcaccttggc cagccggtgc tggctgcgcc gcgtgatcgt
     3661 ctgctggcga aacgcgatcc actcgaccag catctggcgc agcgactttt gcaccggccg
     3721 cccgtcgatg ccgaccatgg tcaggttgat gggcgcggag gtctccaggc tggtgtgcgc
     3781 caagagcgcg gtaatcaagt cctgctgcgg cacgcggccc gtcttgggct cgatgaccag
     3841 gcgcaccggc gcgtccttgc tcgattcgtc gcgcacgccg tcgagcagcg ccagcatgga
     3901 tgccttgagc tgctgctgct cctgcgtgag cgccttcttg ccggtcttga ccttggggtt
     3961 gctgatgtcc tcgatttctt ccagcacctt ttgcgtcgac acgcccggcg gcagctcggt
     4021 gacgaccagc tgccactggc cgcgcgccag gtcttcgatc ttccagcgcg cgcgcacctt
     4081 caggctgccg cgcccgcttt ggtaggcgtc ttgaatgtcg gccgcactgc tgatgatttg
     4141 cccgccgccg gggtaatccg ggccgggtat caggccaaac agctctgcat cggcaaggtt
     4201 cgggttcttc accagggcga tacaggcatc cgccacctcg cgcaggttgt ggctggggat
     4261 ctcggtcgcc aggcccacgg caatgccgct ggcgccgttc aagagagcaa acggcaggcg
     4321 tgccggcagc tggcagggct cctgcgtcga tccgtcgtag ttgggctgga agtccaccgt
     4381 gcccatgtcg atctcggaca gcagcaggct ggtgatgcgc gccaggcgcg cctcggtgta
     4441 gcgcatggcc gccgcgccgt cgccgtcgcg gctgccaaag ttgccctggc cgtcgatcag
     4501 cgggtagcgc tggttgaagt cctgcgccag gcgcaccagc gcgtcgtagg ccgactggtc
     4561 gccgtggggg tgaaaacgcc ccagcacgtc gcccaccacg cgcgcgcttt tcacgggctt
     4621 gggcggggtg ttgccattcg gcccgctcca cgacaggccc atgcgctcca tggcgtacaa
     4681 aatgcgccgc tgcaccggct tcatgccgtc gcagacatcg ggcagggcgc ggcctttgac
     4741 cacggaaagg gcgtattcca gataggcgcg ctgggcgtag ccgcccaggc tgtcgtcggc
     4801 gggcgcggtg gggtctaaaa gggtgtttgc ttgctctgtc atactataaa aaatctagct
     4861 gtttgcgctt aatggataag cgttataggc caaaaacatt cgaatatttc actcggcgat
     4921 accgcccaac tcctgctcaa tctgctcgat gctgggcagg ctcgactcca gggtggcggg
     4981 cagcgcgcgc gtcagctcga agctggagac gccaatgggt ttctcgacac cacgcagcgc
     5041 atattcggcc agcacgcggt tgtgctgctg gcacaggatc aggcccatgg tgggctggtc
     5101 gtgcgcatgg cgcagccggt catcgaccac gctgcagtaa aaattcatct tgcccgcgta
     5161 ctcgggtttg aattcgccgc gcttgagctc gatcaccaca taggggtctt tcagcgtctg
     5221 ctgcaccagg tcggactcgg gcttgggcag gcgcagggca aagttgctcg cggcgcggcc
     5281 ctggcgctgg tgcgcagcgg tctcgatctg catcaccaaa atgctgcggc tccagccatg
     5341 ctccagcgcg gcctgcatgt accagtggcg cgtggcgggg tctttgacct tggccatcag
     5401 gatggcgtga tgcgtccagg gcaaggccaa aatcagctcc gccgtaaaaa gtgccgcagc
     5461 ttgtggcact tttggctcgg tctctatttg tgcagcaggc tgctgcacaa atgacaattg
     5521 cgcgtactcc cggtaaaacg ccagcatctg cttgatgttg cgctcagaaa aacccttctc
     5581 ctctggcaaa tcgttgtgca aatcgcgcgc aagacgggga atcacgcccg cgccccaacc
     5641 ctcttgctgc tggcgctcat caatcacccg gccaatgtcc cagtacaggc gaatcagctc
     5701 ggcattgacc gccagcatgg cgcgcacctg ggcgcgccgc acgcgctgct tgatgtcggc
     5761 cagcagagtg gcgtagagcg ccggcacgtt gggctcaggg ggtgtttcag gtttttttac
     5821 gcgcatggca gcaattcccg cacgccatcc cactgcctgt ccttgaccat cacgaaccgg
     5881 cagcgccccc cggacagttc ggcccacaga ttgccgatca aacggtcatc ctcggcgctg
     5941 ctccagcggt cggctccttt gtactcaacc accagcaccg tgccattggg cagttggcag
     6001 acaaagtcgg gatagaaacg cccatcggcc ttttgcagaa agaacgagca gccttcgcgg
     6061 cgcaccaggt tgcgcaccca gaactggatg cgcccctgct gcgcccacat gtccagctgg
     6121 caggcgcatt caaactcttc cttgctgtcg aagtccccca tgcgcccgta gaaatgtttg
     6181 cgaaagtcga actggccaaa gcgcccgtcg tagtcgcggc tgggtgcgta ggcgtggggc
     6241 gcaaactcaa aggcgtattg atccgagacc gccacgcgca tggccgcatc gtcgccaaaa
     6301 agcgcctgct ggaacgcctt gcccacggcc tgcttgcgca gctcccgaat gcgcgcctcg
     6361 atcagattgc gtatctgaaa tttctgcagg ttggcccgcg ccagcgtgta accgtcacgg
     6421 cgcagcaaat cactaagcca cgccgccacg aaggcctgct tggacgggtg ggtcagcgac
     6481 ggctcaggca ggttgcggca cagccaggtg gcaatgcgca ccgcatccca gttctcgggc
     6541 tgataaacca agcccaaatc acgctgcaac tcgggcagaa agcgggtgaa aaccttgccg
     6601 ctgccctcgt cgatgtcgat cgcaccgcct tccgacacct tcaaggccgc gcccaaggcc
     6661 gacaggtcgg ccgccgtggg cgccgcgtcg tagagcgaga catcccaggg gtagtccagt
     6721 acctccgggt cgtcaaacag caccaattcc ccttgctgga ctggcacccg caaggccagc
     6781 tgcggcacgc gcaagcgctc gcccaactcg gcaggcgtct gaaaaaactc gatcgccgtg
     6841 gtgcgactga cctcggcggc ctgcacgatg gccgccgcag cggctggcga actgacacag
     6901 gccttgagcg tttcggcctc atcctcagtc agcggcgcgc tgatggtcag ggtattgagc
     6961 tggccgtccc aactcacctt gccccgcacc gttttgggca cgctcttcaa gtccggtttt
     7021 tcgggcaagg caaccgccac cgggcgcagc accacgcgcc cggcgtggcc ctccaaatcc
     7081 agccgcgcct ggtcgggcag cacggcggcg acaaactcgg ccacctcgcg ccgttcgaac
     7141 ccggcccccg ccaccagccg gtcgcgcaag gcgcctgccg tctcagcaaa gtcgcgcgac
     7201 accacaaagg cataggactg gttgagcagc ggggatgccc gctggctggc ccccggctga
     7261 cgcagcacgc gcccgagcaa ctgctccacc gccgtgctcg acgacatcga cgccatgctc
     7321 accaaaatgt aggcaaacgg gcaatcccac ccttcagcca gcgctttttg cgtgatgacg
     7381 aacttcaccg ggcaggccgg gtcggccatg cccagcgggt aatcggcgtc gatctgctcc
     7441 agcccctttt cctcgcccgt ggccaccacg atttcactgg ccgggatgcc atggttggcg
     7501 atcaactcct ggcgcagctt gtcaaagtcc agcgtctcaa cccctgcgcg gcgcggctcg
     7561 gactggatca gcaccagggg gcgcaaatac ggcgcacccg cgcggcgctc ctcatcggcc
     7621 agcgcttgca gcgcatcgcg ccggccaatc gcgtccgcca ggcattgctg ccaatggggc
     7681 tcggtttgca gcagcaccgg cagcttgatc atctcctcgg ccttcagctc ggccgccgac
     7741 acactgtgca gcacattgct gggcgtgcgc tgcacgtcgg gcgtggcggt gagctccatc
     7801 accccgctgg ggcgaaagcg cgccagcatg tcaaaggcca gctcggtgcg gctgttgtgc
     7861 gcctcgtcca ccaccacaaa ggggcggcgc aggcgcagca cattggccag cgaatacggc
     7921 gtgctgcgct cggtgccttc accgtcggcc agcaactcgg cgcgctgggc gggcgcgagg
     7981 tggtcaaagt ggtgcatcag cgcgccgctg ctttggtaca ccttgcggca ctcctcctct
     8041 tccacctgaa aggcctggcg cgtggcgacg atgacggtgg tcgtggtgtc cannnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgtcggca ggaaagccag gcagcgggtg
     8221 gtagggattg ccacgccccc acaggcgttc ggtggtggcg gtaaaggcga ttgagggcga
     8281 gggcaactcg tggcaggcct tgaaataagc ctccacgcta tcgagcacct gttgctggta
     8341 ggttttgggg gtaaaggcac tcatgcggac tctgccttgg tttcaggcga gtccagctcc
     8401 ggggtcggcg tcggggtagg ggtcggttgg ggcttgatgt gctccagcac gccattgcgc
     8461 gtcaccacca aatcggtgcc ggtttgcacc gccacctcac gcgcacgctg cgcggcacgc
     8521 tgcaaagcct gccaggagcc gcgcaaatcg ctgctacggg cgttctctaa gggtttgccg
     8581 ttcatcgttc actccaatcc agcaacaccg gcacttcacc ggcgttgtcg tacaaggccc
     8641 agacatccac cgcaggcgcg tagtagcgct ggaaattttc cagccccgcc acaaaacgcc
     8701 ggcgtatcac ttcgggcgga atatcgtgcc cgccctggcg cacccgctgc gccacccgtg
     8761 caacggcctc ttcggcgctg ctcagacgca agaaaatcag ctttacctca taccccgcct
     8821 gctgccagcg cgcgatatgg cgcaaatagg cgcggcccga cagcgtggtt tcaaaggcaa
     8881 agctactacc cgcctgaaag tgccgctcca actcagacaa catcagccgc ccggcctgca
     8941 ccgctgccga ctcgggcgca aaaggcgcca gcccggcggc aatcaaatcg gcattgacga
     9001 acaccgggca agcggcctcg tgcggcaaga actcacgggc gaacgtggtt ttgcccgcgc
     9061 cattgggccc ggcgatgatg atgaccttgc gttcctgtgc ggccatggct cacacctcca
     9121 gcgcgtaggg cgtttgcttg aaggtaatgc cttcgcgcgc cgtgcgactg cccaggcggt
     9181 tggcggcggc gtaaatcacc ttggggccgt caaagggcgg cagcacggcc agcaccgggg
     9241 cggtgagcac gttgccgccg gccacggatt tgtccttgag gatgccgttg tagagcaggt
     9301 aaatggcgcg gccctcatgc acgcccagca ggggcgaatc ggcctgaccg gtgtaaccgg
     9361 tttgggtttc agccaaccag acgaatttag ccagttcggc aaacgtgaca tcgctgcgga
     9421 tttgaccgta ggcgtcgaac aaaggctcag acgacaggcg gcagaactga aaaccgccgc
     9481 ccaggccagc tgcggcctgg cctttggcgt tggtgtagcc accggccaca cgccgcacgc
     9541 gctcggcggt gacgttttgg gcgatgtttt catccatttc aacgaggatg aagtggcgct
     9601 ggccgccgtc ctcggcgttt tgcttgagca cggcgtgggc ggtggtgccg gaaccggcga
     9661 agctgtcaag aacgatggaa tctttatcgg tagcgatttg caggatgcgc tgaatcaaca
     9721 gcgaaggctt gggtgtggcg aaatccgata ccgaaggcac gtcatgcagc acttcgcgca
     9781 gctctttacg tgaaccatcg gtgtgaccgg cgaattcgtg cggccacagc gtttgcggca
     9841 ctacaccatc attgcgcaac gcatgcttgt agcgcttgta ggccggcact tttcccttgc
     9901 catccttgcc ccagtaaatc aaatcctccg ctctatgccg ttgatgctca tcctgcgaat
     9961 aagaccaaac acgggtgcgt ttaggccaaa tttcttcacc ggtgttgggg ttggtgaccg
    10021 ggtaatacag attggggcgt tcttcggcag tcttggcgca tgtgtaatcg ctactacgaa
    10081 attgcccctt ttcatcctca aatttgtact ggctgtcctt ttcttcacca cgcggcagca
    10141 ggttgcgctg ccaactaggt gagcgctggt acaccagcac aaagtcatgc ataggtgcaa
    10201 tggttttgtg gtcgttggcg acggcgtatt ttttctgcca gatgacattg gcaacaaaat
    10261 tacccgaccc aaaaatctca tccatcagca agcgcaacgt ccccacttcg ttgtcgtcaa
    10321 tcgacacaaa aatcgccccg tcctcgcgca aaaactgctt caaaagcacc aggcgcggat
    10381 acatcatgca cagccagcgg tcgtggcgat ccagcgtttc gccctccttg cccaccacct
    10441 cgcccagcca gcggcgaatc tccgggctgt tgacgttgtc gttatagacc cagccctcgt
    10501 tgccggtgtt gtagggcggg tcgatgtaga tgcatttgac ctgccccgcg tagcgcggca
    10561 gcagggcttt cagggcgtgc aggttgtcgc cctgcacgat caggttgccg gtgtccgccg
    10621 cggcgcagga cagcgcagca tcgggctcca gcaggcgaaa cggcacgtct ttgtggtgtt
    10681 tgacgacggc gtccttgccg atccagttca atgtgggcat gggtttttac ttcaaaatca
    10741 atagctgcta gcgcttgctg gataagcgtt agacgtcaaa aacaaaggaa ttccacctcg
    10801 cccgcagcgc ccaccgttaa ccccagctgc gcgtcagcag ttcgtccagc gcgtcgctga
    10861 tggcctggcc ctgctctgcc aggctgccca ccaccggccc gagctgttcc agggcgacgg
    10921 acaccatgtc cagcgggtgc agcaccacgg tttgcccggc gatttcaaaa acggggttca
    10981 tgcgcgtgcc ggccacgcgg ccgccggcgg gcagggccga gcggagcacc agcggcacga
    11041 cgacgcggcg acggtagccg tcgaacaagg ccgactgcac cagcaccacc aggggcacgg
    11101 taccccgctg cgggccgggg ttgaggtgga cgtcaaattg cggcatggga acgggtttta
    11161 cagggtggaa tgcgcgtccg caaaagaacc gcttgcatcg tgcagcgcgt tccagtcggc
    11221 caccgccgct tgcgcctgct gctggtgctg gctgcgcgcc tggcgctgcg cctccacata
    11281 ggcctggagc atttcctcga ccttggccga caggttgccg cagtacgccc gcgactcgcg
    11341 caccagggcc tcgttgagcg acagattgac gggttttttg gcgctggatt ccagggcggt
    11401 catggcttgg ctccttttat gcgcatttta tgcgcatgcc ggttggcaag ccgcccttgg
    11461 aacatgtgca agcccttttc atggcggcca gccgcaccgg caaaaggcgg cgcgtgtcgg
    11521 ggttgcccgg tgggcgcgct ggacttcaca ccatagggcc cggcgctcat gaatcaccga
    11581 tgcagctttt cctgcagcca caccttgatc agcgactgat acggcacgtc ccgtgcgttg
    11641 gccgccgcct tgatggaatc gagcaaatgc tgcggcaggc gcagcgagat ggtcttggtg
    11701 gtgggcttga ggttgggcag caccacgctg tgcgcctgcg accaatcaag atagtcggtc
    11761 gagtcctggc tttcccagaa ggcgcgttct tccgcctcgc tggcaaagtg cgggattgtt
    11821 ttagtgggct tgttcataaa acgctcgctc cttgcggtgc atgtcacggg ccgaaatcac
    11881 gcggatcagg ctgcctgagc cacgcagggt aaaggtcagg tgcagcagcc gaccgtcatc
    11941 gctgcggccc agggcgtggt agcgcggctc ttgctggctg tgcttggcat cgcccaggat
    12001 cagcagcggc gcgttgaaga aaacctgttc cgcctgcgcc atggaaacac cgtgtttgtc
    12061 gttcttgcgc tcattgccgg cgtcccagtc aaaaccggtg atttctgcca gtttcagcat
    12121 attttgtatg ttatagccat atacagtaaa gacaaggccg gatgcttaaa catccacctc
    12181 caccgcatcc ccatgcagct ccatcagctc gcggcgggat gccgcttcac ccttgcccat
    12241 gaggcgggtg atcagcccct cggtggcggc aaagtccatg gcgcccaatt gcacaggcaa
    12301 gaggcgccgg gtgtcggggt tgagcgtggt gtcccacagc tgctcggcgt tcatctcgcc
    12361 caggcccttg aagcggctga tctggcattt ctcgcgcgcc acgccgtcct tggcggcctt
    12421 gtcgaggatg gcctcgagtt cgccctcgtc cagcgcgtac agcttggcgg cgggtttttt
    12481 gccgcgcgcg ggcacgtcca cgcggtagag cggcgggcgc gccacgtaga tgtggccggc
    12541 ctctatcaac ttggggaagt ggcggaagaa cagcgtgagc agcagcacct ggatgtgcgc
    12601 gccgtccacg tcggcgtctg acaggatgca gaccttgccg tagcgcaggc cgctcaaatc
    12661 gggttggtca tggggcccgt gcgggtcgac gccgatggcg accgcaatgt cgtgcacctc
    12721 gttgttcttg aacagcaggt cgcgctcgac ctcccaggtg ttgagcacct tgccgcgcag
    12781 cggcaggatg gcctgcgttt ccttgtcgcg gcccattttg gcgctgccgc cggcgctgtc
    12841 gccctcgacc agaaagactt cgttataggc caggtcgcgg ctttcgcagt cggtcagctt
    12901 gccgggcagc acggccacgc cgctgccttt tttcttctcg accttttgcc cggctctttg
    12961 ccgggtttgt gccgccttga tggcgagttc ggccagcttt ttgccccagt ccacatgctg
    13021 gttgagccac agttcgagcg ctgggcgcac aaagctgctc accagccgca cggcgtcgcg
    13081 cgaattgagc cgctccttga tctggccctg gaattgcggg tccagcacct tggcgctcag
    13141 cacgtagctg gcgcgggcga acacgtcctc gggcatcagt ttcacgccct tgggcaagag
    13201 cgaatgcagc tcgataaagc ccttgacggc ctggaacagg ccatcgcgca agccgctgtc
    13261 gtgcgtgccg ccggcgctgg tggggatgag gttgacgtag ctctcacgca ccggcgcgcc
    13321 gtcttcggta aaggccacgg cccaggcagc gccctcgccc tcggcaaagc tctcgtcgct
    13381 gccgctggca aagccctcgc cctcgaacag cggaatcacc gggtcggcgg caagcgtctg
    13441 cgtcaggtag tcgaccaggc cgcccttgta ctgccaggtc tgggtgtcgc gggttttctc
    13501 gttgatgagc tgcaccgtca cgcccggcat cagcacggcc ttgctgcgca gcaaatgcgt
    13561 cagctcgccc atgggcagct gggcggattc aaagtatttg gcatcgggcc aggcgcgcac
    13621 cgtcgtgccc tgcttgcgct cgcctgtctc cagcggccta gccaccaggg gctcgaccac
    13681 atcgccggcg gcaaaggcca ggcgcgcgat ctggccctcg cggtgcgtgg ccacttccag
    13741 gcgcgtggac agggcattgg tcaccgacac gcccacgccg tgcagcccgc ccgagaagct
    13801 gtaggcgccg cccacgccct tgtcgaactt gccgcccgcg tgcaggcggg tgaacaccag
    13861 ctcgaccacc ggcgcgccct cttccgggtg caggccgaag ggaatgccgc ggccgtcgtc
    13921 ctcgacgctg accgagccgt cggcgtgcag cgtgaccttg atcttcttgc cgtagcccgc
    13981 cagcgcctca tcggcggcgt tgtccagcac ctcctggatg atgtgcaggg gattgtcggt
    14041 gcgggtgtac atgcccgggc gctgcttgac gggctccagg cccttcagga cgcggatcga
    14101 gccttcggaa tatgcgggtg aagtaatcgg tttgactgcc atgggcgcgg attgtagtgc
    14161 tggatataaa gccaggtttt catgctttaa atggccgcgt caggcagtca acccgggttt
    14221 tcgctacatt cgtgcgatgc acgacgcgac gcgaaaactc tccatagccc aggtgctgat
    14281 ctgcggcgcc accatcgtca ccctgtccat gggcatacgc cacggctttg gcatgtggct
    14341 ggcgcccatc acgcaggaca tgggctggac gcgccagacc ttcgccctgg ccatggccat
    14401 tcaaaacctg tcctggggca tttttggcat cttcggcggc atgatggcgg atcgcttcgg
    14461 cgcgtttcgc gtgctgatcc tgggcgccct gctctacgcc ctgggcctgg cgggcatggc
    14521 gctgtcgccc acgccgctgc tgttttcgct gaccaccggc gtgctgatcg gcgcggccca
    14581 ggccggcacc acctatgccg tgatctacgg cgtgctgggg cggcagatct cggccgagcg
    14641 gagatcctgg gccatgggcg tggcggcggc cgcgggctcg ttcgggcagt ttttgatgat
    14701 gccgctcgaa ggccagctga tcgcacaact gggctggcag acggcgctgc tggtgctcgc
    14761 cgccatggtg ctcttgatcg tgccactggc ctttggtctg cgcgagcctg gctttcacgg
    14821 cagcgcgccc atccagcgca cccagtccgc cggccatgcc tttgccgagg cgctgcgcta
    14881 caagagcttt ctgctgctca ccgcgggcta cttcgtctgc ggcttccagg tggtgttcat
    14941 cggcgtgcac atgcccagct acctcaagga ccatggcctg tcgccccagg tggccagcac
    15001 tgcgctggcg ctgatcgggt tgttcaacgt ctttggcacc tacgccgccg gcacgctggg
    15061 ccagcgcatg gccaaacgcc atatcctggc cttcatctac tttgcgcgcg ccgtggtcat
    15121 caccatcttt ctgctggtgc cgctgtcgcc catgtcggtg tatgtgttct cggccgtgat
    15181 gggcacgctg tggctgtcca ccgtgccgcc cacgaatgcc gccgtggcgc agatctttgg
    15241 cgtgcagcac ctgtccatgc tcagcggctt cgtcttcttc agccatcaga tcggcagctt
    15301 ctttggcgtg tggctgggcg gctatctgta cgacaccagc ggcagctacg acatcgtctg
    15361 gtacctggcg atcgccctgg gcgtggccgc cggcctgatc aacctgccgg tgcgcgaagg
    15421 cgcgattgcc cggcccacac cacaggcggc ctgagcatgc gggccaccca caagaaatgg
    15481 cttgccgccg cagctgcggt gctggtgctg ctggcggtgt ttgcgctgta cctgcggccc
    15541 gacttcatgc tgaccctggc cgaccagatc tgggcctgtt tctgatgcac ggcctggctg
    15601 tcccatcgga ctggatccgg cgctggtcgc acctggtgcc tgcgggcggg agcgtgctgg
    15661 acgtggcctg cggccagggc cgacacatgc gctggtttct tgaacgaaat caccccgtaa
    15721 cgggcataga taaagcgcaa gaagctattg aatcaatagc atacctgggt gaggcgatct
    15781 gcgccgacat cgaggccggg ccctggcctc tgcccgggcg ccgctttgac tgcgtggtgg
    15841 tcaccaacta cctgtggcgc ccgctgctgc ccaccatctt ggacagcgtg gcgccgggcg
    15901 gcgtgctgct gtacgagacc tttgcccagg gtaacgagag cgtgggccgg cccgcgcggg
    15961 cggatttttt gctgcgcccg ggtgaattgc tacaggcctt cggggcactg cgcgtggtgg
    16021 cctacgagga cggctttctc gacgcgccgg cacgcttcgt gcagcgcatc gcggccgtgc
    16081 gcgagccgct ggtgcccagc gggcctttgc gcctgccgct ggtgggcacg ccgtagttag
    16141 aatgctcttt taccgttttt gaccgcggac atgacctctt ccagcgccac cctcaccggc
    16201 agcatcgtcg ccctcgtcac ccccatgcac gacgacggca gtgtggacta ccccacgctg
    16261 cgcaaactca tagactggca tatccaggaa ggcaccgatt gcataggcgt ggtcggcacc
    16321 acgggtgaat cgcccacggt gaacgtggag gagcattgcg aaatcatccg cgtgtcggtg
    16381 gagcaatccg ccggccgcgt gcccgtcatg gccggctgcg gcgccaactc cacgtttgaa
    16441 gccatcgagc tggcccgcta cgccaagaag gtcggcgcca acagccagct gcaggtcgtg
    16501 ccctattaca acaagcccac gcaagagggc cagtaccagc acttcaaagc gattgccgag
    16561 gccacgggcg acctgcccat ggtgctgtac aacgtgcccg gccgcagcgt ggccgacatg
    16621 caccacgaga ccgtgttgcg cctgacccag gtgccgggca tcgtcggcat caaggaggcc
    16681 acgggcaaca tcgagcgcgc gcagtggctg atccgcgacg tgcccaaggg ctttgccgtg
    16741 tattcgggcg acgaccccac ggccgtggcc ctgatgctct gcggcggcca gggcaacatc
    16801 agcgtgacgg ccaacgtggc gccgcgcctg atgcacgagc tgtgcgtggc ggccatcgcc
    16861 ggcgaccgcc gccgcgcgat ggacatccag ttccagttgc tgccggtgca caaaaatctg
    16921 ttcgtcgaag ccaaccccat ccccgtgaaa tgggccatgc agcgcatggg cctgtgcggc
    16981 ggcgccatgc gcctgcccat gacgccgctg gcgcagggca acgaggccgt ggtcgaaacc
    17041 gccctgcgcg ccgccggcct gctggccgcg gacaggggct gaagccatct cccccggcgc
    17101 actcctgcgc ccgggtcagt cctggactgt agaaggatta attgcgtgaa cgctaccacc
    17161 cgcctgggcc tgctgggcct tgccatgacc ctgtcggcct gctccgtgct ggagaacgac
    17221 aagatcgact acaaaagtgc caccaagggc gcaacgctgg aggtcccgcc cgacctgaac
    17281 cagctgtcgc gcgagacgcg ctacaccgtg cccggcgcca ccgtctcggc cgctgccttc
    17341 gaggccggcc aggcgcggca gccgcgcggc actgccagcg ccgcgccgca agccatcggc
    17401 gacgtgcgca tagaacgtga cggcaaccag cgctggctgg tggtcaaccg ccccgccgat
    17461 caactgtggg agccggtgcg cgacttctgg ctggaaagcg gcttcgtcct caccgtcgag
    17521 cagcccaagc tcggcctgct ggaaaccgac tgggccgaaa accgcgccaa gctgccgcag
    17581 gacatcatcc gctcgaccat tggcaaggtg ttcgattcgc tgtattccac cggcgaacgc
    17641 gacaagttcc gcacccgcct ggagcgcacg gccggcggcg ccaccgagat cttcatcacc
    17701 caccgcggca tggaagaggt ctacaccagc agccagaagg acaacacgat ctggcagccg
    17761 cgcccggcca acgccgagct ggagaccgag ttcctgcgcc gcctgatggt caagctgggc
    17821 gtgagcgagg agcagtccaa ggccgtcgcg gctgcgccgg tacccgtcac gccgagcgcg
    17881 cgcatggcca cgctgaacaa tgtgcccgtg gtgcagctcg acgaaggctt tgaccgcgcc
    17941 tggcgccgcg tcggcgtgtc gctggaccgc acgggcttca ccgtggaaga ccgcgaccgc
    18001 agccagggcg tgtacttcgt gcgctatgtg gcgcccgggg aaaagaagga acagggcttc
    18061 ttcagcaagc tgttctccag ctcgcccgac gccgtggcac cgctcaagta ccgcatcgtg
    18121 gtgcgcagcg agggcaacca gagcacggtc tccgtgctca acgccacggg ggcacccgag
    18181 acatcggcca acgcgcagcg catcgtgcgc gtgctggcgg acgatctgaa gtaagccgcg
    18241 caaagcggcc aacaaaaaac cccggtcgat tggccggggt tttttgttac tgccaggctc
    18301 gcaagcctgg agcgggagtg cttacttgga cgcgccagcg gctgcattgg cagcagcgtc
    18361 ggcagccttc ttggcggcgt cagcggcagc gtcggcggct tccttggtgg cgtccacagc
    18421 ggcggcggca gcatccttgg tggcgtccac ggcagcagca gcggcatcct tggtggcgtc
    18481 agcagcatcc ttggtggcgt ccacagcggc ggcagcggca tccttggcgg cgtcagcagc
    18541 ttcggcggcg ggggctgcca cgggtgccgg agcaggtgca ggtgcgggct cttccttctt
    18601 gccgcaagcg gcgagggcag cagcagcaat cagggcagcc agaacgacgg agttcttcat
    18661 ttcagtttcc tatgggaaca agtgatagtc atggaaagcc gcgacacccg cctcttgcag
    18721 cggtataccc ggcgacgatg cattgcttga tttaaagcaa acaccaggct taataccagc
    18781 tgccgattat agaaagtttt cgtcaatagt ggataacgcg aagaaaccac tacctttatt
    18841 acaacagtgt gcgcagctca caggcccaag gtggaggcag gaaagccggg agcactttta
    18901 cgctccagcc attcgttgat ggcctcgcgc agcaggatgc gccgctcggg ctcggcgccc
    18961 gtcacgccgt tgcagcgcaa ggggtcgtgg cgctgcaaga cccactgggg cgaccagatg
    19021 gcgctgggca gatcctgcgc atccaccgca ccagcgcggc gacacacaat gatgtgatcc
    19081 cagcgcacgc gccactgcac gaagcgcgcg tggcgccgct gcacctcggc atagtcggcc
    19141 gccagcatgg tcaggcggcg tccgctgctc gcccattgct gcagcgactc gaccacggcg
    19201 cgctcgccca ggggccagtc gtggaagttg gcatcgctca caatcagttc gcgccagccc
    19261 tcctgggcgg cgcaggccag ggcgtcgcgc accagctttt gaaacgcctc gcgcccttca
    19321 aagcggccgc tgggcaatgc cggcgtcgcc tgctcctggt tgggatcagc gtgcatgggc
    19381 ccagccggct tcgcaccatg cggacagcag ctccagcgcc tcgtcgctgg cgcgcaccag
    19441 atcgcgtggc tcaaggcggc gttcgtcggc caggcggcgc atcagcgtgg cgtcgcgccc
    19501 ggaggcgcgg tagctctcgc cattgatgaa gacatgcttg tcgtcgtaca tcatgcgcgt
    19561 gcgcctatcc agcgtcaggc attcgagcat cccgccagcc tcggcgggct cgaaccagac
    19621 attcggcttg ggctcggtca gcgcctcgcc cagcgcacat tcgagcgcct gcggctgcgc
    19681 cagcgcgcgc tgcacggcct ggcgggcaaa gtcctgcagc tcggccggaa tggcaccggg
    19741 cccggccacg gccggctgct gcgcgtcgcg gtagatggca ccctcctcat cggcagcgcc
    19801 ctcggccatg cgctgcagca cttcgtgcgc cagctgggcg ccgttgggcg agcgaaaacc
    19861 gatcgagtag gtcatgcatt cgcccacggc gatgccgtca tgcgcccagc ccgggggcag
    19921 gtagagcatg tcgccgggct ccagcacatg ctcctcctct gcctcgaagg ccgccaggat
    19981 cttgagcggc aggccggggc gcagcgtctt gtccttttgc cggcctatgc gccagcgtcg
    20041 cttgccgtgc gcctgcagca ggaacacgtc gtagttgtcg aagtgcgggc cgacgccgcc
    20101 gccgtcggtg gcgtagctga tcatcaggtc atccaggcgc gcctcgggca caaaacggaa
    20161 ctgctgcatc agcgcgtgca cgccctcgtg gtgcagatcc acgccctgca ccagcgccgt
    20221 ccagcgcggc gtggccagcg acggcagcga acggcgtgcc agcggcccgt ggcgcagcac
    20281 ccactgcccg cccttgtcct ggatgaagcg cgactccacg ccctcgcgtc cggccagctc
    20341 gagcagctct gcgcgggcga gcagcggctt gaagccggca atcgcctggc gcaccagcaa
    20401 aggtttctta tgccagtggc ggcgcatgaa ctgggccggg ctcaggcctc cgagcagggc
    20461 caaaggttgt tgtacatcca tgggatagac gcggccgcgc gggccttgag gctgtgaact
    20521 gcgacaattg tcctatggaa ataaccgaac aatgtgtggt cgccctgacc tgggtcttga
    20581 aggacacgct gggtgaggag ctggacgtgc tcgacgatcc ggtggagttt ctggtgggtg
    20641 gcgacgacct gctggcgcgc atcgaggagg ccctgcaggg ccattgcgtg ggcgccgcgc
    20701 tggcgctgca cctggagccc gaggacgcct ttggcgacta ccaggacaag ctgctgttcc
    20761 tggagccgcg ccatctgttc cccgacgaca tcgaggaggg catgaccttc gagggcgcgg
    20821 ccctgcccga aggcatcaac cccgaggcgc cgcgcgacgc gctgtacacc gtggcccaga
    20881 tctatcccga gcatgtggtg ctggacggca accaccctct ggccggcatt gcactgcgcc
    20941 tggcgctcaa ggtgcatggc gtgcgcgaag ccaccgagga agaaatcggc cgcggcacgg
    21001 ccggcacagg gttctttcgc atccagcccc aggcgccggg caacgacctg ctgcattgag
    21061 cagccaccgg caggctcaat gcttgccggt ggagccgtag ccgccctcgc cgcgctcgct
    21121 ggcgggaaac tcggtcacca cattgaactg cgcctgcagc accggcacga tgaccagctg
    21181 cgccagccgc tccatgggct gcagcgtgaa ggcctggtcg ctgcggttcc aggcgctgac
    21241 catcagctgg ccctggtagt cgctgtcgat cagccccacc agattgccca gcacgatgcc
    21301 atgcttgtgg cccaggcccg agcgcggcag gatcagcgcc gcgtagcccg ggtccttcag
    21361 gtagatggcg atgcccgtgg gcacgagctg ccaggcattg ggcgccagct ccaggggcgc
    21421 gtccaggcag gcgcgcagat ccagcccggc gctgcccggc gtggcgtagg tgggcaactg
    21481 ttccgccatg cgcgggtcga gaattttgac gtctatcttc atgggtgcaa gggaagtaat
    21541 tcaacggtcg cggccacctc ggccgtgcct ggatttccat acgccatggc ccacgcccgc
    21601 cactatcacg ccggccatga caaagggcaa cagcgcgggc gcaaaccaca tcagcagcac
    21661 cagcagcgcg ccgagcaaca gcggccattt ggccgacaga gtgcgcgtga cggcctggtt
    21721 tgcgccctgc ttcgcggggc ggcgcggagc agtcgtggcg gcagcgggcg ccgccgcctt
    21781 ggcaaccgcc acaggccgct gcccggcggc cgccccggcc tgcaatacgc cacgccgcag
    21841 gtcctggctc tgcaacaggc gctcgatata gcgagcgtag tcgccaccgg gcggctcgcc
    21901 gtagtcgcca agccagggct gtggtgcgct catgaacggg gcgcgccggc ctcacccagg
    21961 cgccgtgcga tctcggccac cagctggcgc ccgagctcgg acttgggcgc gcgcggcagc
    22021 tcgcggtggc cctgtgcgtc caccagcagc agcgcgttgt cgtcctggcc gaaggtggcc
    22081 gggccaatgt tgcccaccag cagcggcacg cccttgcgca cgcgcttggc gctggcgtgc
    22141 gccagcaggt tctcgctctc ggcggcaaag cccacgcagt acagctggcc ggcgcgtgca
    22201 cgcccggatt gcgccacctg ggccaggatg tccgggttct ccacgaaggc gagcgtcggg
    22261 gtgtcgcccg agccatcctt cttgatcttg tgctcggcgg catgggccgg gcgccagtcg
    22321 gccacggccg cggtagctat gaaaatagta gcttccggcg cttgtgtatc aaccgcttga
    22381 agcatgtttt gtgctgaacg cacatcgatg cggcgcacgc cgcgcggcgt gggcaggtgc
    22441 accgggccgg ccaccagcgt gacctcggcc cccgccgcgc gcgccgcgcg ggcaatggca
    22501 aagcccatct tgcccgacga caggttggtg atgccgcgca ccgggtctat ggcctcgaac
    22561 gtcggcccgg cggtgatcag cacgcgctgg cccacgagca gcttgggggc aaagaacgct
    22621 tgcagctcct ccaggatttc ctcggcctcc agcatgcggc cgtcgcccac ttcaccacag
    22681 gcctgctcgc cacaacccac gcccagcacc gtggcgccgt cctgcgccag ctgggccagg
    22741 ttgcgccgcg tggccgggtg ggcccacatc tcgcggttca tggccggggc gatgagcagc
    22801 ggcacgcgct ccatcggccg ggccaggcac atgagggaga gcagctcgtc ggcgcgcccc
    22861 tgcaccaggc gcgcgatgaa gtccgcgctg cacggcgcaa tgaggatggc atcggcctcg
    22921 cggctgaggt tgatgtgcgg catgttgttg gcctcgcgcg catcccactg cgagcggtag
    22981 acgctgcgcc ccgacagcgc ctgcatggtc acgggcgtga tgaactgctc ggccgcctcg
    23041 gtcatcacca cctgcaccgt ggcgccggcc ttggtcagca gccgcgtcag ctcggcactc
    23101 ttgtagcagg ccacgccacc gctcaggccc aggacgatat gtttgccgga aagctcattc
    23161 atgggcgcca atttagcaga cggcacccag gggcaaggcg gtgcgggcgc cgccgccctg
    23221 gccggacttc ctataatcgc aatttcccac atccactcaa aaagatgacc aaattcgtct
    23281 tcgtcaccgg cggtgtggtg tcttccctgg gcaagggaat cgcctcagcc tcccttgccg
    23341 cgatcctcga atcgcgcggt ctcaaagtca ccctcatcaa gctcgacccg tacatcaacg
    23401 tggatccggg caccatgtca cccttccagc atggtgaggt cttcgtgacc gacgacggcg
    23461 ccgagaccga cctggacctg ggccattacg agcgtttcat cgaaacgcgc atgaagcagg
    23521 ccaacaactt caccaccggc cgcatctacc agagcgtgct cgagaaagaa cgccgcggcg
    23581 actatctggg caagacggtg caagtcatcc cgcatgtcac gggcgagatc caggagttca
    23641 tcaagcgcgg cgccggcatc ggcacggcgg acgccgtgga cgtggccatc tgcgaggtcg
    23701 gcggcacggt gggcgacatc gagtccctgc ccttcctgga ggccgtgcgc cagctgtccc
    23761 tgaagctggg cccgaacaac tcggccttcg tgcacctgac ctacctgccc tggattgcca
    23821 cggccggtga attgaagacc aagcccaccc agcacacggt gcagaagctg cgcgagatcg
    23881 gcatccagcc cgacgccctg ctgtgccgcg cccaccatgc cgtgcccgac gaggagcgcg
    23941 agaagatctc tctcttcacc aacgtgcagg aatggggcgt gatcagcatg tgggacgtgg
    24001 acaccatcta caaggtgccg cgcatgctgc acgagcaggg gctggacggc ctgatctgcg
    24061 acaagctgcg cctgaacacg ccgccggcca acctgaagcg ctgggacgat ctggtgcgcg
    24121 agtacgaaca cccgcagggc gaggtgcaga tcgccatggt gggcaagtat gtggaactgt
    24181 cggacgccta caagtcggtg aacgaggcgc tcaagcacgc cggcatgcaa agccatatgc
    24241 gcgtgaagat cacgcatgtc gattccgaga ccatcaccga cgccaacgcc gccgagcagc
    24301 tgggccagta ccacgccatc ctggtgccgg gcggctttgg ctcgcgcggg gtcgagggca
    24361 agatcagcac ggcacggtac gcccgcgagc acaaggtgcc ctacctgggc atctgcctgg
    24421 ggatgcaggt ggccaccatc gagtacgcgc gccatgtggc gggcctggac ggcgccaact
    24481 ccaccgagtt cgacgcgcac tgccagcacc cggtgatcgc cctgatcacc gagtggaagg
    24541 acgaggacgg cagcatcaag acgcgcgacg agaactccga cctgggcggc accatgcgcc
    24601 tgggcgcgca aagctcggac gtgcaggccg gcacgctggc gcacagcatc tacggcagcg
    24661 tggtcaccga gcgccaccgc caccgctacg aggccaacgt gcagtacctg gatcagctgc
    24721 gcaaggccgg cctggtgatc tcggcgctga cgcagcgcga gcacctgacc gagatcgtgg
    24781 aactgccgca aagcgtgcac ccctggtaca tcggcgtgca gttccacccc gaattcaagt
    24841 ccacgccctg gaacggccac ccgctgttca acgccttcgt gaaggcggcc attgcgcacc
    24901 aaaaggcatg aaagggagca gtacccatgc aactctgcgg ctttgacgtt ggcctggatc
    24961 aacgcttctt cctcatcgcg ggcacctgct ccatcgagag cctgcagctg tcgctcgacg
    25021 tcgcaggcca gctcaaggag gcctgcaccg ccctgggcgt gccgctgatc tacaagggct
    25081 cgttcgacaa ggcgaaccgc tcgtcgggca gcagccagcg cggcgtggga atcgacgccg
    25141 gcctgaagat cctggccgag gtgcggcgcc agctgcagct gcccatcctg accgacgtgc
    25201 acgaggccgc gcaggtggcc gaggtggcca gcgtggtcga cgtgctgcag acgccggcct
    25261 ttctgtgccg ccagaccgat ttcatccgcg cggtggcgca gtccggcaag ccggtgaaca
    25321 tcaagaaggg ccagttcctg gcaccctggg acatgaagaa cgtcatcgac aaggcacgcg
    25381 acgcagcgcg cgaggtgggc ctctccgaag accgcttcct ggcctgcgag cgcggcgtga
    25441 gctttggcta caacaacctg gtggccgaca tgaccagcct ggccgagatg cgcaactccg
    25501 gcgcgccggt ggtgttcgac gtgacgcact cggtgcaaaa gcccggcggc ctgggggccg
    25561 tgagcggcgg cgcccgcgcc atggtgccgg tgctggcgcg cgcagccgtg ggcgtgggcg
    25621 tggcggggct gttcatggaa acccacccca ggcccgccga ggcctggtcc gacggcccca
    25681 atgccgtgcc gctcaggcat atgcgtgcgc tgctggagac cctggtggcg ctggatcagg
    25741 tcaccaagaa gaacggcttt ctcgaagacc actttgacgc ctgaaagcct gacaggacgc
    25801 gcccagggcg aaaccattgc gcccgcaatg cacgcaatgg cgcgcgccgc atcaccgata
    25861 ctcgcggctg cggcccaggc cagaatacta tgctattgat agctgcttgc gcttgataca
    25921 taagctctac agccatattt gaccgcaaat cacccgatac cgatttcaag caacccagaa
    25981 ggaaactcca tgagtgccat tgttgacatc gtaggccgcg aagtgctgga cagccgcggc
    26041 aaccccac
//
LOCUS       DAOMTQ010000026        25864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_196_length_25864_cov_20.202604, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000026 DAOMTQ010000000
VERSION     DAOMTQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 25864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25864
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_25864_cov_20.202604"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PK518_04365"
     CDS             <1..637
                     /locus_tag="PK518_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM48176.1"
                     /translation="ALLVTALQLRARARTPAATAARLTELQSRIRPHFLFNTLNSAIA
                     LVRDEPAKAEALLEDLSDLFRHALVEQGESATLEEEITLARRYLDIEQVRFGERMRVQ
                     WQIDPRAHAARLPPLLLQPLVENAVKHGVEPSAHGGKLRITAEQRGSRVVLTITNTLP
                     PESTSGEQRPRGHGIAQANVRERLRLLHDVDCDFSAGMHNGLYQVRISLPC"
     gene            655..1398
                     /locus_tag="PK518_04370"
     CDS             655..1398
                     /locus_tag="PK518_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM48177.1"
                     /translation="MHILIVDDELLARSRLRTLLGDCDARHQVSEAAHAADALAQLNI
                     SGGRAVDLVLLDIHMPGQDGLALAQQIRRLPHPPTIVFVTAHADHALSAFELDAVDYL
                     TKPVRLQRLQQALAKAQRASGQTVIAPAVAPDERDTLLIQERGRTERVPLAEVLFLRA
                     EQKYVTVRTATRSFIVDDALSELEARHAAHFLRVHRSTLVARRAMRALERHYDEAEGE
                     GWAVRLQGLTELLPVSRRQVAAVREALAG"
     gene            complement(1402..2265)
                     /locus_tag="PK518_04375"
     CDS             complement(1402..2265)
                     /locus_tag="PK518_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRM48178.1"
                     /translation="MTLGAIILATLAAGIGSVWLAAALLRLGLGGRSRVGPQHLLSLA
                     AGALLATAFTLILPEAFEGPVDAQNLFTTLLLGLVFFFLLDKAELWHHGHEHSPAERQ
                     LHGHGTSHAHAEHGHQHDHHDHHDHHDHDHHHHHGHGGWSLLTGDSVHCFGDGILIAS
                     AFVADMRLGVVAALSVLAHEVPHHIGDLVVLRHSSHHRNAALLKVSLAGAVTALGGMV
                     GYYLVGLWDGALPYFLTIAASSFIYVALADLIPQLQKRLSAAQTLAQIAWLAAGIIAV
                     TVIGALAHHGH"
     gene            complement(2373..3257)
                     /locus_tag="PK518_04380"
     CDS             complement(2373..3257)
                     /locus_tag="PK518_04380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRM48179.1"
                     /translation="MDQYLHHDLHALLPGRGVASRRHALGLVLGAGYAAAAAPVLAQQ
                     AIATPVDGLVAGEVSFAVNGAQVYAYRAAPQGRTGLPVVLVVSEIFGVHEHIADVCRR
                     LAQAGYLAIAPDLFARQGDPMAYGEVAKLMSELIAKVPDAQVMADLDGAVQWAGAHGG
                     DLNKVAITGFCWGGRITWLYAAHGPVKAGVAWYGRLVGQASALNPKHPVDIAAILKAP
                     VLGLYGEKDGGIPLDTVDRMKAALKEGSAAAKASEFVIYPDAPHAFHADYRPSYRQDA
                     AQDGWRRMLGWFKHNGVV"
     gene            3394..5739
                     /locus_tag="PK518_04385"
     CDS             3394..5739
                     /locus_tag="PK518_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRM48180.1"
                     /translation="MTSTATPSPPQASRFGSGQAVPRLEDAQLLRGEGRYVDDFAPDG
                     LAHLVFVRSPHAHARLRGVDADAARALPGVLAVYTGADLVAAGIKPLPGPGFKRADGG
                     PGASPARRPLAHEQVRFVGEPVAAVVAQTLQQALDAAEAVFVDYEELPAATSVADALG
                     PGAPQLTPQAPDNIAAAMRHGDAGACAQAFARAAQVVHLAVSNQRVAAVTLEPRSLLA
                     WVDAADGRLLLRMSTQMPSGVRKLVCDCLGLPSQQVRVLVGDVGGGFGMKTSAYPEDV
                     ATAWAAWQLRRPVKWVAQRGEDFLSSAHGRDVVSQAEMALDAQGRILALRLHSQANVG
                     AYATPTGVAIQLMIGPWVQTSVYDIPVIDFHFQAVLTNTASTGAYRGAGRPEAIYLME
                     RLIDEAARQTGIDRLSLRRRNFIRPEQMPYTNAMGQQYDSGRFERVMDQALPLADWQG
                     FAARAAASQARGLWRGLGIATFLEWTGGNAFEERVTVTIQADGVVEVFSAVNAMGQGI
                     ATSLAQLVVDAFGIPIEQVRVVLGDTDRGDGFGSAGSRSLFTGGSAMRTGAERAIEHG
                     RELAAQALEASVHDVQYQAGRYQVAGTDLGLNLAELAARQPERQIFVDSTSAVAGPSW
                     PNGCHICEVELDPQTGAVQVVRYASVNDVGRVVNPLIVRGQLEGGAVQGLGQALSEAV
                     AYDAASGQLLSGSLMDYALLRALDTPPFQMTMDESTPCLNNPLGVKGVGELGTIGATP
                     CVVNAVADALARAGRADLAPRLTMPLTPGRLWQLLQPQPRG"
     gene            complement(5867..6631)
                     /locus_tag="PK518_04390"
     CDS             complement(5867..6631)
                     /locus_tag="PK518_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRM48181.1"
                     /translation="MSLFSYEVHNRVAEILFNNPPVNALTEAMLRDFLDALERAARDP
                     EVRAVIIGSRVPGRFSAGLDLTAVHQGAHQKVRALLDRLYVRMTEVQFQMGKPTIAAV
                     GGTARGGGMTIAISCDMIVASSDATFGYPEIDAGVLPSIHFTHLPRIVGKHRAFELLF
                     TGRAFDAQEAQSLGLVSRLTEPGQALEGARALAQTLCGKSPEVVRFGRNAFMLANDNG
                     YRQAVAAAADSFCNVAMTDAAREGIAAFVEKRKPRW"
     gene            complement(6655..7848)
                     /locus_tag="PK518_04395"
     CDS             complement(6655..7848)
                     /locus_tag="PK518_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRM48182.1"
                     /translation="MQPLKGLKVVDLTKVLAGPLCGQYLGEYGADVIKVETVGGDDTR
                     RWLPQVQGESAIFVAVNHNKRSIAVDLKTQQGRDIVHRLVKDADIVLQGFGGGTAKKL
                     GVDYETLCALNDRLIYCEISGFGRNGPLGNAPGYDVMLQAFSGMLSTMGQPEGDYARA
                     SFSPVDIGTAMNTLSGVLAAVIERGRSGRGVYLEVSLLDTALGFMTYMAQSYWQTGVN
                     PRPMGTAHPSMVPYQAFEAQDGAIMLGAGNDAQWQRFCAVAGLQDWVAHPDFATNALR
                     VANHAKVVALLQPVMKSRTAAHWLTALEQANVPCSPIHTLGQALAHPQVQARAIIAQT
                     EHPVLGPVQNIGHPVQFAHQPRAASRPAPLLGEHTQEILRQLGYGAQQIADLEQQGVV
                     AAASH"
     gene            complement(7876..8850)
                     /locus_tag="PK518_04400"
     CDS             complement(7876..8850)
                     /locus_tag="PK518_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48183.1"
                     /translation="MHQVTRRVLLAAALAAALPLQAMAQSGAGKITTLVVPFAAGGPT
                     DFMARLLAQPLGRELGTQVIIENRPGASGNIGTKQVMTTKPDGLTLVHTTIAMQAINP
                     LMYPDQDFNPARDLVPVGITGALPNVLVVHPSSGIKTLAELVAKGKAKNAQLNFATFG
                     PGSSPHFYGTLFQKATQVAAEPIAYKGSGDAAKDMLAGRIDYMFDSMTTAIANVKAEK
                     LVALAITSPQRSPLLPDVPTLKELGYGAADVNFWLTLQVPAKTPPEMVAALRQAVSKA
                     VQDPEYKHNVAARGVENLYVAPERLDAFFVSETKKWRDTALSIGIKPE"
     gene            8982..9977
                     /locus_tag="PK518_04405"
     CDS             8982..9977
                     /locus_tag="PK518_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM48184.1"
                     /translation="MRLDLRALRSFVAVASAGSISSAAQSLHIAQPALSVQIKQLEEQ
                     LGAALFDRHPRGVVPTAVGERLLGHAVEILRRVDVAYDDVRQAVDQPSGRVAIALPQS
                     VANFVTVPLVQAVVRQWPLIQLQMVELSSGYIPQQLLHGLVDIGITFGVEDDARMRFT
                     HVLQEDLVFITTAQQLAQYQGASAGGPAHVALPDVARFPLVLPTTTQSLRRVLDAYMV
                     KAGVAPRIVVEVNTIHEILALVAAGMGSTILSFAAVSDLLAAGQLLALPIARPAMSRS
                     IYTCRSATLPMSIAATKVHEQLLATITALASSRRWPQATVMLDRPPGDGSAGPPG"
     gene            complement(10039..10563)
                     /locus_tag="PK518_04410"
     CDS             complement(10039..10563)
                     /locus_tag="PK518_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRM48185.1"
                     /translation="MARIPYADCTSAAARPLADRIAAERGSVLHLYQMLLHSAPVAEG
                     WLGYLTAIRQKSSLPGDLRELVIMRVAQLNHAPYEADQHAPIALREGVSQAKLDALPN
                     WQDCPLFDARERAVLAYTDAMTRQVQVSDEVFAFVAAHFEPRRLVELTATVAAYNMVS
                     RFLEALQIHSHDAR"
     gene            11091..12416
                     /locus_tag="PK518_04415"
     CDS             11091..12416
                     /locus_tag="PK518_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM48186.1"
                     /translation="MNTRTAPPSSDPIRKHLETARTQIGNGELREAAQTLNTAQRIWP
                     GDARIYMLAGLMAEKSGNLKGALEAMRKSVAAAPDWGPGLLELALLLARQNQFKEAVE
                     TAEKVAALEPKNLMVLAGVVDIAHRAGHKEMAIKHLRRGLVLVPGDLQLSQLLAQDLS
                     ALNQHEEALHLWTALIEANPKNEPALLGRVKTLLAAGKPAKAVADTTTLLALAPGDSV
                     YAYYNALAHGITPAHQPVELNRQLFDGMAEVYDQHTVRGLRYQLPKIVGDKILARYPE
                     KRLNVLDLGCGTGLLGVCLGRIDGFLIGVDVSTKMIEQAARHNVYDRFHTVNLLEALE
                     ATPAALYEVITALDVFIYTGDLGQTIPNAHRILLPAGDLYFSCETAPEDGPDLVLQPN
                     GRYAHKRSHVHALCRQAGFESVEIEDLVLRYEAGEPVQGFVVTARKAAA"
     gene            complement(12551..13834)
                     /locus_tag="PK518_04420"
     CDS             complement(12551..13834)
                     /locus_tag="PK518_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRM48187.1"
                     /translation="MAVTLFIAVVVLLALGFPVAFALAISAALAVFVGGRYPQLVVFK
                     EMFTGIDSFPLMAVPFFILAAELMSGGALTAVLLRFAAQFVGHLRGGLGYANVLSLTL
                     FSGISGSALADAAGPGSMMVKMMDKAGYARSYAAALTASTAIVGPIIPPSVSMIIYAL
                     QDENVSVGGLFVAGFVPGILIAIAMAAVNWWVCKKRDYRSREPRPSGREILVNSFKAI
                     PALLLIVLIIVGIRFGIFTPTEASVVAVFYALVCGKWVYRTLQWKAVPAIAARSAMLT
                     SSVLLVMATSAAFAWVLTVEGIPQYLSQLIVSWELSPVMFLVAVNVLLLVFGIFMEPL
                     PGVMILVPILAPIASALGIDPTHFAMVVIVNLTLGMITPPVGGLLFVTAVSTRVSITA
                     LTREMPPFLLAHFVVLALLTFVPQLSTWLPHSLGF"
     gene            complement(13851..14366)
                     /locus_tag="PK518_04425"
     CDS             complement(13851..14366)
                     /locus_tag="PK518_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRM48188.1"
                     /translation="MLDRVERALVACNRWLLIVLLLAMACIVFANVVLRYTTGDSIVW
                     AEEVARHLMIWGTFLGAGLVLRFGGHVAIDNLHNAVSTRTARVIRMVVVAGVGLFCLV
                     MAYFSVLYVWATRFQTTAATDVPMAWIYVAMPVGFGLMFVHLLFIARGYIAAGSYVES
                     GEMGADAAASI"
     gene            complement(14463..15461)
                     /locus_tag="PK518_04430"
     CDS             complement(14463..15461)
                     /locus_tag="PK518_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRM48189.1"
                     /translation="MTPKKFSRRALVAACAASAFAFPAGLVCAQQAKEVKLGYALAVN
                     SHYGAAAQAWADSVEKASNGAFKFKQFPSSALGGERELIEGLQLGTVEAIIVSTGALS
                     NFVPDVGVVDIPFLFRDTAHARAVLDGAFGQDLLGKFQKRGLVALAWGEQGFRHLTNN
                     KHPVQKIEDLKGLKIRVTENPVHITAFKTLGASPTPMSWPEVIGALQQGTIDGQENPV
                     SVLASTKLWQVQKHLTLSAHVYAPVALIVSPGFWGGLTDAQKSAFTQGAKLGALASRA
                     FVDAVEKKGVDEAKSHGMQVVMGVDQAAFRAALEPAYKQYATKFGQKTLDAIAAVK"
     gene            complement(15592..16149)
                     /locus_tag="PK518_04435"
     CDS             complement(15592..16149)
                     /locus_tag="PK518_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRM48190.1"
                     /translation="MKVLKLSGQGLPQSWISLEQAVTHYAAGEVRWEIGAQVALFRGG
                     HNAVTGEQSQIAVSSIIGTRGVPHINPFDLRPSLTNAKLFTRDRNICAYCGGRFHEED
                     LTREHIVPFASRGLNHWMNVVTACRACNHRKGPRTPEQAHMPLLYTPYVPSLWEDFIL
                     RNRRILADQMEFLMAHVPKSSRLLS"
     gene            16315..17358
                     /locus_tag="PK518_04440"
     CDS             16315..17358
                     /locus_tag="PK518_04440"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRM48191.1"
                     /translation="MKIFRGFHHPQVASACALTIGNFDGVHRGHQAMLALLNNEAQHR
                     GVASCVLTFEPHPRDYFARVHHKPDIAPARIGTLRDKLAELARCGVQQAVVLPFNDKL
                     ASLPAQAFIDEVVVRGLGARYLLVGDDFRFGSQRAGDYAMLDAAGPAGGFEVARMMSY
                     EVHGLRVSSTAVRAALAAGDMAEAARLLGRPYAISGHVVHGRKLGRQLGRSAASGADQ
                     DGFRTLNLRFAHWKPAASGIFAVLVHGLHDAPLPGVANLGVRPSLDPRDANGGRVLLE
                     THCLDWPTHLGAEGGYGKIVRVELLHKLHDELKYDSLDALTAGIARDCEDARAFFATA
                     HAETRRQTTRDRI"
     gene            17453..20308
                     /gene="ileS"
                     /locus_tag="PK518_04445"
     CDS             17453..20308
                     /gene="ileS"
                     /locus_tag="PK518_04445"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRM48192.1"
                     /translation="MSENKTPKPEASAYRATLNMPDTPFPMRGDLPKREPQWAKDWSE
                     QGVYKKLRDARRDAPKFILHDGPPYANGQIHIGHAMNKILKDMIVKSRQLEGFDALYT
                     PGWDCHGLPIENAIEKKHGRNLPRDEMQARGRAFATEQIAQQMADFQRLGVLGEWDNP
                     YQTMAYASEAGELRALKKVMERGFVYRGLKPVYWCFDCASSLAEFEIEYQDKQSQTLD
                     VMFPAADPARLAAAFGLPALTKEAFIVIWTTTAWTIPANQALNVNPELEYSLVDTERG
                     LLLVASALVEKCLERWKLQGQVLATALGKKLDLMPFKHPLAHVDKAFDRISPVYLADY
                     ATAEDGTGIVHSAPAYGVDDFNSCVAHGLKYEDILNPVQGNGVYAPELGMFGGQHIWK
                     AVPVIIDALKVAGRLLDSITISHSYPHCWRHKTPVIYRAAAQWFVRMDEGDGVFTDPK
                     QKPAQTLRQIALAAIENTHFYPENGKARLRDMIAGRPDWCISRQRSWGVPLPFFLHVD
                     SGELHPRTMEIMDQAAEIVEKGGIEAWSRATAEEILGAEDAKLYTKSTDILEVWFDSG
                     STFWHVLRGTHAGMHHDEGPEADLYLEGHDQHRGWFHSSLLLASAIFGRAPYRGLLTH
                     GFTVDGQGKKMSKSLGNTVSPQEVSGKLGAEIIRLWCAATDYSGDLAIDDKILARVVD
                     SYRRIRNTLRFLLANVSDFDPAKDSVPFGDMLEIDRYALSRAAEFQANLLAHYKVYEF
                     HPVVAKLQLYCSEDLGGFYLDVLKDRLYTTAPKSLARRSAQTALYQITHAMLRWMAPF
                     LSFTAEEAWKVFGDSESIYLETYQTIPAADEGLLAKWARLREIRDAVNKEIEVLRADG
                     QVGSSLQANVTLTAAPEDHALLASLGDDLKFVFITSAIELVAGSALQISVRASSYAKC
                     ERCWHYRSDVGQDAAHPTLCGRCTSNLHGDGESRVHA"
     gene            20308..20820
                     /gene="lspA"
                     /locus_tag="PK518_04450"
     CDS             20308..20820
                     /gene="lspA"
                     /locus_tag="PK518_04450"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRM48193.1"
                     /translation="MPAARSGPSMWPWLAWAVVLLIADQVTKTLILNNYRLGDSTFIT
                     GFFNIVRAHNTGAAFSFLADAGGWQRWLFTGIGAVATIFIVWQLRAHPGQKLFCFALA
                     SILGGAVGNVVDRLMHGYVVDFLDFHWNFLSGMFPGGHFPAFNLADTAISVGAACLIL
                     DELLRVRRER"
     gene            21005..22657
                     /locus_tag="PK518_04455"
     CDS             21005..22657
                     /locus_tag="PK518_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRM48194.1"
                     /translation="MKHLLNLLAAVSLLIWGTYLVRTGILSVFGANLRQLIARSVSSR
                     FTAVLSGIGVTAVVQSSTATALIVSSFVGQGLVGLPAALAVMLGADVGSSVMAVVFSL
                     DLSWLSPLFIFAGVVLFTARNGTAVGRVGRVLIGLGLMLLALRLITESTIVLTQSPTV
                     RTLLSALTSDLLLELFTGALLAIVAYSSLATVLLTAALAGSGGIPPDVALGLVLGANL
                     GSGVLAVLTTLKANVQTRQVPLGNLFFKTIGVVIMTPLTGLWLQHVRPYVPETATLVV
                     LFHLAFNMVVGLLCISLTGTVARLVQRLLPQDSAPTVGARLQHLDPSALATPSLAISC
                     AAREALHQADVVESMLLGLHKVIRTDDLQLAEDLRKLDDQVDSLYSAIKYYMTKISRS
                     ELNEVEGQRWTEIISFTINMEQIGDIIERVLIDIEDKKIRKSRQFSEAGMEEINELHA
                     RLISNLRLAMSVFLNGSVRDAQKLLEEKARFRDLEHAYSVTHLARLSDNTVQSIETSS
                     LHIDLISDLKRINSLLCSLAYPILESAGALAPSRLKPLQDQG"
     gene            complement(22673..23494)
                     /locus_tag="PK518_04460"
     CDS             complement(22673..23494)
                     /locus_tag="PK518_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM48195.1"
                     /translation="MTAPGLDGWLAAARRAAQQPPAQPRQTLVVAGQVVGSVAPDFLN
                     KISLQRLLDKRYQLLNQEYGGAPAWQLDVPPEAATDALNTLAAALRDADLCGPWRNEQ
                     LAVCNAAGAVIATVERGAVRVLGLATRAVHLVGLAPDGRMWVQQRSMTKPNNPGQWDT
                     LMGGMVSAADTLHEALARETWEEAGLRVAALHGLRHGGHVDFGRESREGGGAGYMRER
                     IDWFCAEVPEGMAPDNQDGEVERFDLLAPQAVRQWLAQGRFTLEAGLVIAAFLGL"
     gene            complement(23491..24474)
                     /locus_tag="PK518_04465"
     CDS             complement(23491..24474)
                     /locus_tag="PK518_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRM48196.1"
                     /translation="MSLAVQIRQPGGAEELHLVDVAVGEPGPGEIRIRHHAIGLNFID
                     VYHRTGLYPLNLPATIGMEAAGVVEAVGEGVTHLKVGDRAAYASQPPGAYCEARVMPA
                     KCVCKLPDAISFETGAAMMLKGLTAQYLLKKTLPVEGLQPGDHVLFHAAAGGVGLIAT
                     QWAKALGLQLIGTAGSDAKCQLVRDNGAAHAINYNTEDFAARVKEITGGKGVKVVYDS
                     VGKDTWDKSLDCLRPFGLMASFGNASGPVPPFAPGSLGAKGSLYVTRQTLFTHIATRE
                     ATQAMADDLFAVVASGQVKIHIAQRYPLAEVQQAHRDLEARKTTGSTILTL"
     gene            24568..25467
                     /locus_tag="PK518_04470"
     CDS             24568..25467
                     /locus_tag="PK518_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM48197.1"
                     /translation="MTQRLTPGTVALLVTAPLLWAGNAVVGRLVHDLVPPITLNFLRW
                     ALALALLLPLTHRVLRPDSPLWPHWRRYALLGLLGVGLYNALQYLALQTSTPINVTLV
                     GSSMPVWMLAVGALFFGVAVTRRQLAGAALSMIGVLTVLSRGEWSQLLALRLVAGDLF
                     MLLATISWSFYSWLLVRTSEPASVRGDWAAFLTGQMVFGLAWSGVFASAEWATGHTHI
                     AWGWPLVAALLFIAIGPALVAYRCWGIGVQRAGPAVAGFFSNLTPLFAALLSAAFLGE
                     APSGYHAVAFALIVGGIVVSSRR"
     gene            complement(25562..>25864)
                     /locus_tag="PK518_04475"
     CDS             complement(25562..>25864)
                     /locus_tag="PK518_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM48198.1"
                     /translation="TGFVAGAARSKLAAQGVTLNNLLPGKFDTDRLATTLGAAATKSG
                     KSLDDVRRAQQAQIPAGRFGNPEEFGAICAFLCSVHAGYMTGQNVLADGGAYPGTF"
BASE COUNT         4379 a         8830 c         8461 g         4194 t
ORIGIN      
        1 ggccctgctg gtcacggccc tgcagctgcg cgcgcgcgca cgcacgccgg cggccaccgc
       61 cgcgcgcctg accgagctgc agtcgcgcat acgcccgcat ttcttgttca ataccttgaa
      121 cagcgccatt gccctggtac gcgacgagcc cgccaaggcc gaggccctgc tggaagacct
      181 gagcgacctg tttcgccacg cccttgtcga gcagggcgaa tcggccacgc tggaagaaga
      241 aatcaccctg gcgcggcgct acctggacat agagcaggtg cgttttggcg agcgcatgcg
      301 cgtgcagtgg cagatagacc cacgcgccca cgccgcgcgc ctgccgccgc tgctcttgca
      361 gccgctggtg gaaaacgccg tcaagcatgg cgtggaaccc agcgcccatg gcggaaaact
      421 gcgcatcacc gccgagcagc gtggcagccg cgtggtgctg accatcacca acaccctgcc
      481 gcccgagagc accagcggcg agcagcgccc gcgcggccac ggcatcgccc aggccaatgt
      541 gcgcgagcgc ctgcgcctgc tgcacgatgt ggactgcgac ttcagcgccg gcatgcacaa
      601 cggcctgtac caggtgcgca tttctctccc ttgttgaccg gattgaaagg ccccatgcac
      661 atcctcatcg tggacgacga acttctggcc cgcagccgcc tgcgcaccct gctgggcgat
      721 tgcgacgcgc gccaccaggt cagcgaagcg gcgcacgccg ccgacgcgct ggcgcagctc
      781 aacatcagcg gcgggcgcgc cgtcgatctg gtgctgctgg acatccacat gccgggccag
      841 gacgggcttg cgctggcgca gcagatcagg cgcctgccgc acccgccgac catcgtcttc
      901 gtcaccgccc atgccgacca tgcgctgtcg gccttcgagc tcgacgccgt ggactacctg
      961 accaagcccg tgcgcctgca gcgcctgcag caggccctgg ccaaggcaca gcgcgccagc
     1021 ggtcagaccg tgattgcgcc agcagtcgca ccggacgaac gcgatacgct attgatacag
     1081 gagcgcggcc gcaccgagcg cgtgccgctg gccgaggtac tgttcctgcg cgccgagcag
     1141 aagtacgtca ccgtgcgcac cgccacacgc agcttcatcg tggacgacgc gctgtcggag
     1201 ctggaggcgc gccatgcggc gcacttcttg cgcgtgcacc gcagcaccct ggtggcgcgc
     1261 cgcgccatgc gcgcgctgga gcggcattac gacgaggccg agggcgaggg ctgggccgtg
     1321 cgcctgcagg gcctgaccga gctgctgccc gtctcacgcc gccaggtggc ggcagtgcgc
     1381 gaggcgctgg cgggctaacg ctcaatgccc atggtgcgcc agcgcaccaa tcacggtcac
     1441 ggcgatgatg ccggccgcca gccaggcgat ctgcgccaac gtctgcgcgg cgctcaatcg
     1501 tttttgcagc tgcggaatca agtccgccag ggccacataa ataaagctgc tggcagctat
     1561 tgtgaggaag tacggcagcg ccccgtccca caggcccacc aggtagtagc ccaccatgcc
     1621 gcccagcgcc gtcacggcgc cggccagcga caccttgagc agggccgcat tgcggtggtg
     1681 gctgctgtgg cgcagcacca ccaggtcgcc gatatggtgc ggcacctcgt gcgccagcac
     1741 cgacagcgcc gccaccaccc ccaggcgcat gtcggccacg aaggccgagg cgatcaggat
     1801 gccgtcgcca aagcagtgca cgctgtcgcc ggtcagcagc gaccagccgc catggccgtg
     1861 gtgatggtgg tgatcgtggt cgtggtggtc gtggtgatcg tggtgatcgt gctgatggcc
     1921 atgctcggca tgcgcatggc tagtgccatg gccgtggagc tgccgctcgg ccgggctgtg
     1981 ctcgtggccg tggtgccata gctcggcctt gtccagcaga aagaagaaca ccagccccag
     2041 cagcagggtg gtgaacaggt tctgcgcatc gaccggcccc tcgaaggcct caggcagtat
     2101 gagcgtgaag gccgtggcca gcagcgcgcc cgcggccagg ctcagcagat gctgcggccc
     2161 cacgcgcgag cgcccgccca gccccaggcg cagcagcgcc gccgccagcc acacgctacc
     2221 aatgcccgcg gccagggtgg ccagaatgat tgctcctaat gtcatagctg tttgcgccct
     2281 accctattgg gttagaggcc tttttcataa aaccatcaaa aaaagccccg aggggctttt
     2341 gctgtccttt gccgttgctg cggcgtaccg cgtcacacca cgccattgtg cttgaaccag
     2401 cccagcattc ggcgccagcc gtcctgcgcg gcatcctgcc ggtagctggg ccggtagtcg
     2461 gcatggaagg cgtggggcgc atcggggtag atcacgaact cgctggcctt ggctgcggcc
     2521 gatccctcct tcaacgccgc tttcatccta tcaaccgtgt caaggggtat gccgccatct
     2581 ttctcgccat acaggcccag caccggcgcc ttcaggatgg cggcaatgtc caccgggtgc
     2641 ttaggattca gcgcactggc ctgccccacc agccggccat accaggccac gcccgccttc
     2701 accgggccat gggccgcgta cagccaggtg atgcgtccgc cccagcaaaa gccggtgatg
     2761 gcaaccttgt tcagatcgcc gccatgggcg cccgcccatt gcacggcgcc gtccaggtcg
     2821 gccatcacct gggcgtcggg caccttggca atcagctcgc tcatcagctt ggcgacctcg
     2881 ccataggcca tggggtcgcc ctggcgcgca aacagatccg gggcaatcgc cagatagccg
     2941 gcctgcgcca ggcgccggca gacatcggcg atgtgctcgt gcacgccaaa aatctccgac
     3001 accaccagca ccaccggcag gccggtgcgc ccctggggcg cggcgcggta ggcgtagacc
     3061 tgggcgccgt tgacggcaaa gctcacctcg cccgccacca gcccgtcgac gggcgtggca
     3121 atcgcctgct gcgccagcac cggcgcggcg gccgcggcat agcccgcgcc cagaaccagg
     3181 cccaaggcgt ggcggcggct ggccacaccg cgtccgggca gcagggcgtg caggtcgtgg
     3241 tgcaagtatt gatccatggc ggtttcctcc aaaagactgg gcaaggccgc gattgtagga
     3301 aggccggtgc cgccgcgcac ggcacggcac gccgcgggca gattggctac agtggccgca
     3361 tgcgccatgg gcacagccac aggagacccc gccttgacca gcaccgccac cccctcccct
     3421 ccccaggcca gccgctttgg cagcggccag gccgtgccac gcctggaaga cgcacagctg
     3481 ctgcgcggcg agggccgcta cgtggacgac tttgcgccgg acggcctggc gcacctggtg
     3541 ttcgtgcgct cgccccacgc ccacgcccgc ctgcgcggcg tggatgcgga cgcggcgcgc
     3601 gccctgcccg gagtgctggc cgtctacacc ggcgccgacc tggtggccgc aggcatcaag
     3661 cccctgcccg gccccggctt caagcgcgcg gatggcggcc ccggcgccag ccccgcgcgc
     3721 cggccgctgg cgcatgagca ggtgcgcttt gtcggcgaac ccgtggccgc cgtggtggcc
     3781 cagacgctgc agcaggcgct ggacgcggcc gaggcagtct ttgtggacta tgaggagctg
     3841 cccgccgcca ccagcgtggc cgacgccctg ggcccgggcg caccgcagct cacgccccag
     3901 gcccccgaca acatcgccgc cgcgatgcgc catggcgacg ccggcgcctg cgcccaggcc
     3961 ttcgcgcgcg ccgcgcaggt ggtgcacctg gccgtcagca accagcgcgt ggccgcggtg
     4021 acgctggagc cgcgctcgct actggcctgg gtggatgccg ccgatgggcg cctgctgctg
     4081 cgcatgagca cgcaaatgcc cagcggcgtg cgcaagctgg tctgcgactg cctggggcta
     4141 ccaagccagc aggtgcgcgt gctggtgggc gacgtgggcg gcggttttgg catgaagacc
     4201 tcggcctacc ccgaggatgt ggccaccgcc tgggccgcct ggcagctgcg gcgcccggtg
     4261 aaatgggtgg cgcagcgcgg cgaggatttt ttgtcgtcgg cccacgggcg cgacgtggtc
     4321 agccaggccg agatggcgct ggatgcgcaa ggccgcatcc tggcgctgcg cctgcactcg
     4381 caggccaacg tgggcgccta cgccacaccc acgggcgtgg ccatccagct gatgatcggc
     4441 ccctgggtgc agaccagcgt ctacgacatc ccggtgatcg actttcactt ccaggccgtg
     4501 ctgacgaaca ctgcgtccac cggcgcctac cgcggcgcgg gccggcccga ggccatctac
     4561 ctgatggagc ggctcatcga cgaggccgca cgccagaccg gcatagaccg cctcagcttg
     4621 cgccggcgca acttcatccg tcccgagcaa atgccctaca ccaacgccat gggccagcag
     4681 tacgacagcg ggcgcttcga gcgggtgatg gaccaggccc tgccgctggc cgactggcag
     4741 ggttttgccg cgcgcgccgc cgcctcgcag gcacgcggcc tgtggcgcgg gctcggcatc
     4801 gccacctttc tggaatggac cggcggcaac gccttcgagg agcgcgtcac cgtcaccatc
     4861 caggccgacg gcgtggtcga ggtgttctcg gccgtcaacg ccatgggcca gggcattgcc
     4921 acgtcgctgg cgcagctggt ggtggacgcc ttcggcatcc cgatcgaaca ggtgcgcgtg
     4981 gtgctgggcg acaccgatcg cggcgacggc ttcggcagcg ccggctcgcg ctcgctgttc
     5041 accggtggct cggccatgcg cactggcgcc gagcgcgcca tagagcatgg gcgcgaattg
     5101 gccgcgcagg cgctggaggc ctcggtgcac gatgtgcagt accaggcggg gcgctaccag
     5161 gttgccggca ccgacctggg gctgaacctg gcggaactgg ccgcgcgcca gccagagcgg
     5221 cagatcttcg tggacagcac cagcgccgtc gccggcccgt cctggcccaa cggctgccat
     5281 atttgcgagg ttgaactcga cccgcagacc ggcgccgtgc aggtcgtgcg ctacgccagc
     5341 gtgaacgacg tgggccgggt ggtgaatccg ctgatcgtgc gcggccagct tgaaggcggc
     5401 gccgtgcagg gcctgggcca ggcgctgagc gaagcagtcg cctacgacgc ggccagcggc
     5461 cagctgctct ccggcagcct gatggactac gcgctgttgc gggcgctgga cacgcccccg
     5521 ttccagatga cgatggacga gtccaccccc tgcctgaaca acccgctggg cgtgaagggc
     5581 gtgggcgagc tgggcacgat tggcgccacg ccctgcgtgg tcaacgccgt ggccgacgcc
     5641 ctggcacgcg ccggccgcgc ggatctggcg ccacgcctga ccatgccgct gacgccgggg
     5701 cggctgtggc agttgctgca accgcagccg cggggctgac gccaatactg ttcaggttgc
     5761 gaccgatgcc atctgttcgt aggagcggct tcagccgcga acggcgccat tcgcggctga
     5821 agccgctcct acagcctcga taaatgaaca gtattggggc tgacgctcac cagcgcggct
     5881 tgcgcttctc gacaaaggcg gcaatgccct cgcgcgccgc gtcggtcatg gccacgttgc
     5941 aaaaactgtc ggccgcggcc gccaccgcct ggcggtagcc gttgtcattg gccagcatga
     6001 aggcattgcg cccaaagcgc accacctcgg gcgacttgcc gcacagcgtc tgcgccagcg
     6061 cgcgcgcgcc ctccagtgcc tggcctggct cggtcaggcg cgagaccagg cccagcgact
     6121 gcgcctcctg cgcgtcgaag gcacggcccg tgaacagcag ctcgaacgcg cggtgcttgc
     6181 cgacgatgcg cggcaaatgc gtgaagtgga tcgagggcag cacgccggcg tcgatctcgg
     6241 ggtagccgaa cgtcgcgtcc gacgaggcaa cgatcatgtc gcaggagatg gcaatcgtca
     6301 tgccgccccc gcgcgccgtg ccgcccacgg ccgcgatcgt gggcttgccc atctggaact
     6361 gcacctcggt catgcgcacg tagagccggt cgagcagcgc acgcaccttc tggtgcgcgc
     6421 cctggtgcac ggccgtgaga tccaggcccg cagaaaaacg gcccggcacc cggctgccga
     6481 tgatgacggc acgcacctcg ggatcgcgcg cggcacgttc cagcgcatcc aggaaatccc
     6541 tcagcatggc ctcggtcagg gcgttgaccg gcgggttgtt gaacaggatt tccgccaccc
     6601 ggttgtggac ttcgtagctg aacaaagaca tttcttttcc tttacttctt ccaatcaatg
     6661 ggacgcagcc gcgaccacgc cctgttgctc caggtcggca atctgctgcg cgccgtagcc
     6721 cagctgccgc aggatttcct gcgtgtgctc gcccagcaac ggcgcggggc gcgaggccgc
     6781 ccggggctgg tgcgcgaact gcaccgggtg gccgatgttt tgcaccggcc cgagcaccgg
     6841 atgctcggtc tgcgcgatga tggcgcgcgc ctgcacctgc gggtgcgcca gggcctggcc
     6901 cagcgtatgg atgggcgagc agggaacatt ggcctgctcc agggcggtca gccaatgcgc
     6961 cgccgtcctg ctcttcatca cgggctggag cagcgccacc accttcgcgt ggttggccac
     7021 gcgcagggcg ttggtggcga agtccgggtg cgccacccag tcctgcagcc ccgccacggc
     7081 gcaaaagcgc tgccactgcg cgtcgttgcc cgcgcccagc atgatggcgc catcctgcgc
     7141 ctcaaacgcc tggtagggca ccatcgaggg atgggccgtg cccatcggcc tggggttgac
     7201 gccggtctgc caatagcttt gcgccatata ggtcatgaag cccagcgcgg tatcgagcag
     7261 cgacacctcc aggtacacgc ctctaccact gcgcccgcgc tcgatcaccg ccgccagcac
     7321 cccgctgagc gtgttcatcg ccgtgccgat atcgaccggc gaaaagctgg cgcgcgcata
     7381 gtctccctcg ggctggccca tggtgctgag catgccgctg aaggcctgca gcatcacgtc
     7441 gtagccgggg gcgttgccca gggggccgtt gcgcccgaaa cccgagattt cgcagtaaat
     7501 cagtctgtca ttcagtgcgc acaaagtctc gtagtccacc cccagctttt tcgccgtgcc
     7561 gccgccaaag ccctgcagca cgatgtcggc atccttgacc aggcgatgga cgatgtccct
     7621 gccctgctgc gtcttcaggt cgaccgcgat gctgcgcttg ttgtggttga cggcgacaaa
     7681 aatcgccgat tccccctgga cctgcggcag ccagcggcgc gtgtcgtcgc cccccaccgt
     7741 ctccaccttg atcacatcgg cgccgtactc gcccaggtac tggccgcaca gcggtccggc
     7801 cagcactttg gtcagatcga cgaccttcag gcctttcaat ggctgcatgg ctcgctcctt
     7861 cactgcacgc acggcttatt cgggcttgat gcctatggac agcgcggtgt cacgccactt
     7921 tttcgtctcg ctgacgaaga aggcatcgag ccgctcgggc gccacgtaca ggttttcgac
     7981 accgcgcgcc gccacgttgt gcttgtactc gggatcctgc accgccttgg agacggcctg
     8041 gcgcagcgcg gccaccatct cgggcggtgt cttggccggc acctgcagcg tcagccagaa
     8101 gttcacgtcg gccgcgccat agcccagttc cttgagcgtg ggcacgtcgg gcagcagcgg
     8161 cgaacgctgg ggcgaggtga tggccagcgc caccaacttc tcggccttca cgttggctat
     8221 cgccgtcgtc atgctgtcaa acatgtagtc gatccgcccg gccagcatgt ccttggccgc
     8281 gtccccgctg cccttgtagg caatgggctc ggcggcaacc tgggtggctt tctgaaacag
     8341 agtgccgtaa aaatgcggcg agctgcccgg cccaaaggtc gcaaaattca gctgtgcatt
     8401 ctttgccttg cccttggcga ccagctcggc aagggtcttg atgcccgacg atgggtgaac
     8461 caccagcaca ttcggcaggg cgccggtgat gcccacgggt accaggtcgc gcgcggggtt
     8521 gaagtcctgg tccgggtaca tcagcgggtt gatggcctgc atggcgatgg tcgtgtgcac
     8581 cagggtcagg ccgtccggct tggtggtcat gacctgcttg gtgccgatat tgccgctggc
     8641 gcccgggcgg ttctcgatga tgacctgcgt ccccaactcg cgccccagcg gctgggccag
     8701 caggcgggcc atgaagtccg tcggcccgcc cgcggcaaag ggcacgacca gcgtcgttat
     8761 ttttcctgcg cccgattgcg ccatcgcctg caaaggcagg gctgcggcca gagccgccgc
     8821 caacaacacc ctgcgcgtca cctgatgcat agccatctcc ttgaaaagca tctcacgaat
     8881 cacgaaggcg gcagtctgcg tggccggcag ctatttgaca aagacaggct tcgtatctca
     8941 cccataataa aaaaatatgg ataagttctt gcacatcggc catgcgcctt gaccttcgcg
     9001 ccttgcgcag cttcgtcgcg gtcgcctcgg ccggctcgat ttcatcggcg gcacagagcc
     9061 tgcacatcgc ccagcccgca ctgagcgtgc agatcaagca gctggaggag cagttgggcg
     9121 cggcgttgtt cgaccggcac ccgcgcggcg tggtgccgac ggccgtcggc gagcgcctgc
     9181 tgggccatgc ggtggaaata ctgcggcgcg tggacgtggc ctacgacgac gtgcgccaag
     9241 cggtggacca gcccagcggc cgggtggcga ttgcgctgcc gcaatcggtg gccaatttcg
     9301 tcaccgtgcc gctggtgcag gcggtagtgc gccagtggcc cctgatccag ctgcagatgg
     9361 tggagctgag ctcgggctat atcccgcagc agctgctgca cggcctggtc gacatcggca
     9421 tcacctttgg cgtcgaggac gacgcgcgca tgcgcttcac gcatgtgctg caggaggatc
     9481 tggtgttcat caccacggcc cagcagctgg cgcagtacca gggcgcctcg gccggtggcc
     9541 ccgcccacgt ggccttaccc gacgtggcgc gctttcccct ggtactgccg acgacgacgc
     9601 aaagcctgcg ccgcgtgctc gacgcctata tggtcaaggc cggcgttgcg ccgcgcatcg
     9661 tggtcgaggt caacaccatc cacgagatcc tggccctggt ggccgccggc atgggatcga
     9721 cgattctgtc gtttgccgcc gtcagcgacc tgctggccgc cgggcagttg ctggccctgc
     9781 ccatagcccg cccggcgatg tcccgctcca tctacacctg ccgctcggcc accctgccaa
     9841 tgtcgatcgc ggcaaccaag gtgcacgagc aattgctcgc caccatcacc gcgctggcgt
     9901 ccagccgccg ctggccgcag gccacggtga tgctggaccg cccaccaggc gatggcagcg
     9961 ccgggccacc gggctgaaaa cccggtctcc ggtcacagca ccctgcccgc ccggcggtca
    10021 gcacctgccc cagggccgtc aacgggcgtc gtgcgaatgg atctgcagcg cctccaggaa
    10081 gcgcgaaacc atgttgtagg ccgccaccgt ggccgtcagc tctaccagtc gccgcggctc
    10141 gaaatgcgcg gcgacaaagg cgaagacctc gtcgctcacc tgcacctgcc gcgtcatcgc
    10201 gtcggtgtag gccagcacgg cgcgctcgcg cgcatcgaac aacgggcagt cctgccagtt
    10261 gggcagcgcg tccagcttgg cctgcgaaac gccttcacgc agcgcgatgg gcgcgtgctg
    10321 gtcggcctcg tagggtgcgt ggttgagctg cgccacgcgc atgatgacca gttcgcgcag
    10381 gtcgcccggc aggctggact tctggcgtat ggccgtgagg tagccgagcc agccctcggc
    10441 caccggcgcg ctgtgcagca gcatctggta gaggtgcagg acactgccgc gctcggcggc
    10501 gatacggtcg gccagcgggc gcgcggcggc gctggtgcag tcggcgtagg ggatacgggc
    10561 catggggtgc tctccacaaa tcggggggaa agtgtgcact gcggatcggg atgggatgcc
    10621 atcagtgggc gtggcggcga tcgaccctga gcagccaacc aattacaagc cgcaaaactg
    10681 tcacctcatg agcaccgcac cccattgccc agattttttc cccagtactc aggttattgt
    10741 tagtatttgt aacgcattcg ctcacgaatc tgccccggca accgtgtggc gcgaaacgca
    10801 ggagggcggc gggtttcatg gctgaattta gtactgtgtt tttgtactgt tgatttccac
    10861 ccagaactga cccacctgca cgggtaattt ccatccaaat ctgacccacg taagaaccct
    10921 aacctgctgc ttttagtggc aggagtacag gagtgattga cgtggcgaca ttgagtgtca
    10981 tcaggcgccg gtactgcctc atccacacat gactgataca tggctgataa ttgctggttt
    11041 tcttgactgt ggagcggcgc gctatgtctt gccaccacct gcccaccacc atgaacaccc
    11101 gtaccgcccc gccctcgtcc gaccccatcc gcaaacacct ggaaaccgct cgtacccaga
    11161 tcggcaacgg agaactgcgc gaggctgccc agaccctgaa taccgcgcag cgcatttggc
    11221 ccggtgatgc ccgcatctac atgctggcag ggttgatggc ggaaaaatcc ggcaacctca
    11281 agggcgccct ggaagccatg cgcaaaagcg tggccgcggc gcctgactgg ggcccgggcc
    11341 tgctggagct ggcgctgctg ctggcgcgcc agaatcaatt caaggaggca gtggaaaccg
    11401 cggagaaggt ggccgcactg gagcccaaaa acctgatggt tctggccggc gtggtggaca
    11461 tagcccatcg cgcgggccat aaggagatgg ccatcaagca cctgcgccgc ggccttgtgc
    11521 tggtgccggg cgacctgcag ttgagccagt tgctggcgca ggatttgagc gcgctgaacc
    11581 agcacgaaga ggctctgcat ctatggacgg cacttatcga ggccaacccc aaaaacgagc
    11641 ccgcgctgct cggccgggta aagacgctgc tggcagcggg caagccggcg aaggcggttg
    11701 ccgacaccac cacgctgctg gctctggcgc cgggggacag cgtgtacgcc tactacaacg
    11761 ccctggcgca tggcatcacc cctgcgcacc agccggtgga actcaaccgc cagttgttcg
    11821 acggcatggc cgaggtgtat gaccagcaca cggtgcgcgg cctgcgctac cagctgccca
    11881 agatcgtggg cgacaagata cttgcgcgct acccggaaaa gcgcctcaac gttcttgacc
    11941 tgggctgcgg taccggcctg ctgggcgtgt gcctggggcg tatcgatggc tttttgattg
    12001 gcgtggatgt ctccaccaag atgatcgagc aggctgcgcg ccacaacgtc tacgaccgct
    12061 ttcacaccgt gaacctgctg gaggcgctgg aggccacgcc agccgccctc tatgaggtga
    12121 ttacggcgct ggacgtgttc atctacaccg gcgacctggg ccagaccata cccaacgcgc
    12181 accgcatctt gctgcccgct ggcgacttgt atttctcttg cgaaaccgcc cccgaggatg
    12241 gccccgacct ggtgctgcag cccaatggcc gctatgccca caagcgcagc catgtgcatg
    12301 cgctgtgccg ccaggccgga tttgagagcg tcgaaatcga ggatttggtc ctgcgctacg
    12361 aggcgggcga acccgtgcag ggttttgtgg tgacggcgcg aaaggccgca gcctgaaact
    12421 accagtgtcg catgagcggc ctcagccgcg aatgctgctg ttcgcagcgg cacatgaaga
    12481 tttgctgccc tggttcagcc acgcccaaca aaagtccccg caggtttgtc gcctgcgggg
    12541 gggcttgtat tcagaacccc agggagtgcg gcagccaggt ggacaactga ggcacgaagg
    12601 tcagcagcgc cagcaccacg aaatgcgcca gcaggaacgg cggcatctca cgcgtcagcg
    12661 ccgtgatgga cacacgcgtg gaaacggcgg tgacgaacag caggccgccc acgggcgggg
    12721 tgatcatgcc cagcgtcagg ttgacgataa cgaccatggc gaaatgcgtc ggatctatgc
    12781 ctaaagcgct ggcgatgggc gccagtatgg gcaccaggat catcacgccc ggcagcggct
    12841 ccatgaagat gccgaacacc agcagcaaca cgttcaccgc caccaagaac atcacgggcg
    12901 acagctccca gctgacgatg agctgcgaca ggtactgcgg aatgccctcc accgtcagca
    12961 cccacgcgaa ggccgccgag gtggccatga ccagcagtac cgaagacgtc agcatggccg
    13021 agcgcgccgc aatcgccggc acggccttcc actgcagcgt gcggtagacc cacttgccgc
    13081 agaccagggc gtagaacacc gccaccaccg acgcctcggt gggcgtgaag atgccgaagc
    13141 gtatgccgac gatgatgagc acgatgagca gcagcgccgg aatcgccttg aagctattga
    13201 ccaatatctc gcggccgctg gggcggggct cgcgcgagcg gtagtcgcgc tttttgcaca
    13261 cccaccagtt gaccgccgcc atggcgatgg cgatcaatat gccaggaacg aagccggcga
    13321 cgaacaggcc gccgaccgag acgttctcgt cctgcagcgc atagatgatc atgctgaccg
    13381 aaggcgggat gatgggcccg acgatggccg tgctggccgt aagcgccgcg gcgtaggaac
    13441 gcgcgtagcc ggccttgtcc atcatcttca ccatcatcga gccggggccg gccgcatcgg
    13501 ccagggccga gcccgagatg cccgagaaca gcgtcaacga caacacgttg gcatagccca
    13561 ggccgccgcg cagatggccg acgaactgcg cggcaaagcg cagcagcaca gcggtgagtg
    13621 cgccgcccga catcagttcg gcggccagta tgaagaaggg cacggccatc agcggaaagc
    13681 tgtctatgcc ggtgaacatc tccttgaaca ccaccagttg cgggtagcgc ccacccacga
    13741 acacggccaa ggccgccgag atggcgagcg cgaaggccac cgggaaaccg agggccagca
    13801 gcacgacgac ggcgatgaac agcgtgacag ccatatgtct accctgccct tcaaatggag
    13861 gccgccgcgt cggcgcccat ctcgcccgac tcgacatagc tgcccgcagc gatgtagccg
    13921 cgtgcaatga acagcaggtg cacgaacatc agcccaaagc ccaccggcat ggcgacatag
    13981 atccaggcca tgggcacgtc ggtggcggcc gtggtctgaa agcgcgtcgc ccagacatag
    14041 agcaccgaga aatacgccat caccagacag aacaggccca cgcccgcgac gacgaccatg
    14101 cgtatcacgc gtgctgtgcg cgtgctcacg gcgttgtgca ggttgtctat ggccacatgg
    14161 ccgccaaagc gcagcaccag accggcgccc agaaaggtgc cccagatcat caggtgacgc
    14221 gccacctcct cggcccagac gatcgagtcg cccgtggtgt agcgcagcac cacgttggca
    14281 aacacgatgc aggccatggc cagcagcaaa acgatgagca gccagcgatt gcaggccacc
    14341 agggcgcgtt ccacgcgatc gagcatatgc gggtcggcag gccggggcgt gccgctttct
    14401 tcatcaagca aaagaatccg cgcgggcgga gccgggcgga ttctttcagg acaacaacgg
    14461 acttatttca ccgcggcaat ggcatccagc gtcttctgcc cgaacttggt ggcgtactgc
    14521 ttgtaggccg gctccagcgc agcgcggaag gcggcctgat ccacacccat caccacctgc
    14581 atgccgtggc tcttggcctc gtccacgccc ttcttctcca ccgcatcgac aaaagcgcgc
    14641 gaggccagtg cgcccagctt ggcaccctgc gtgaaggcgc tcttctgcgc atcggtcagg
    14701 ccaccccaga agccgggcga gacgatcagc gccaccggtg cgtagacatg ggcgctcagc
    14761 gtcaggtgct tctgcacctg ccacagcttg gtcgaggcca gcaccgagac cggattttcc
    14821 tgaccgtcga tggtgccctg ttgcagcgcg ccaatcacct cgggccagga catgggcgtg
    14881 ggcgaggcgc ccagggtctt gaaggcggtg atatgcaccg ggttctcggt cacgcggatc
    14941 ttcaggccct tcagatcctc gatcttctgt accgggtgct tgttgttcgt caggtggcga
    15001 aagccctgct caccccaggc cagcgcaacc aggccacgct tttggaattt gcccagcaga
    15061 tcctggccga aggcgccatc gagcaccgca cgggcatggg cggtgtcgcg gaacaggaac
    15121 gggatgtcca ccacacccac atcgggcacg aagttgctca aagcgccggt ggaaacaatg
    15181 atggcctcaa cggtacccag ttgcagcccc tctatgagct cgcgctcgcc gcccagtgcg
    15241 ctggacggga actgcttgaa cttgaaggcg ccattgctcg ccttttccac agagtctgcc
    15301 caggcctggg cagcggcgcc gtagtgcgaa ttgacggcca gtgcgtagcc gagcttcacc
    15361 tccttggcct gttgggcaca gaccagacct gcgggaaagg caaacgccga tgcggcgcag
    15421 gccgcgacca gggcgcggcg agaaaatttc ttgggggtca tgcgaagaaa cgggttgacg
    15481 gttgaagggc ggtgaacggg gcattctaga tttccaccat cgccccagcc ctagtgaaac
    15541 tacgtgcccg gccccacgaa cgacgatgcc ggcgcgcacc gcatgccagc ctcaactgag
    15601 cagccgcgac gatttgggca catgcgccat caaaaactcc atctggtcgg ccaggatgcg
    15661 gcggtttctc aggatgaagt cttcccacag gctgggcacg tagggcgtat acagcaaggg
    15721 catatgcgcc tgctcgggcg tgcgcgggcc cttgcggtgg ttgcaggcgc ggcaggcggt
    15781 gaccacgttc atccagtggt tcaggccgcg gctggcgaag ggcacgatgt gctcgcgcgt
    15841 caggtcttcc tcgtgaaagc gtccgccgca ataggcgcag atgttgcggt cgcgcgtgaa
    15901 cagcttggcg ttggtcaggc tggggcgcag atcgaagggg ttgatatgcg gcacgccgcg
    15961 cgtgcctatg atgctgctga ccgcgatctg cgactgctcg ccggtgacgg cgttgtgccc
    16021 gccgcgaaac agcgccacct gggcgccgat ctcccagcgc acctcgcccg cggcgtaatg
    16081 cgtcactgcc tgctccagtg atatccacga ttgcggcaac ccctggcccg agagcttcaa
    16141 gaccttcacc acccgcctcc tgtcatgcaa cacaagaatg gaatgacaac gttgatgctt
    16201 cgccggccgc cccatgcggt gcgccggtag cgcacaatat acccctttcg caacaaacag
    16261 gcgtcaagcg cttgatggac ttgcgccaaa cgctattaaa agtaaagcac tgcgatgaag
    16321 atcttccgcg gcttccatca tccgcaagtg gccagcgcct gcgcgctcac cataggcaac
    16381 ttcgacggcg tgcaccgcgg ccaccaggcc atgctggcgc tgctgaacaa cgaggcgcag
    16441 caccgcggcg tggccagctg cgtgctgacc tttgagcccc acccgcgcga ctactttgcc
    16501 cgcgtgcacc acaagccgga catcgccccg gcacgcatag gcacgctgcg cgacaagctc
    16561 gccgagctgg cgcgctgcgg cgtgcagcag gccgtggtgc tgcccttcaa cgacaagctg
    16621 gccagtctgc cggcccaggc cttcatcgac gaggtggtgg tgcgcggcct gggcgcgcgc
    16681 tacctgctgg tgggcgacga ctttcgcttt ggcagccagc gcgcaggcga ctatgcgatg
    16741 ctggacgccg ccggcccggc cggcggcttc gaggtggcgc gcatgatgag ctacgaggtg
    16801 catggcctgc gcgtctccag caccgccgtg cgcgcggcgc tggccgcggg cgacatggcc
    16861 gaggcggcgc gcctgttggg ccggccctac gccatcagcg gccatgtggt gcatgggcgc
    16921 aagctcgggc gccagctggg ccgctcggcc gccagcgggg cggatcagga cggttttcgc
    16981 accctcaacc tgcgctttgc gcactggaag ccggccgcca gcggcatctt cgcggtgctg
    17041 gtgcatggcc tgcacgatgc gcccctgccc ggcgtggcga atctgggcgt gcgcccctcg
    17101 ctcgacccgc gcgacgcgaa cggcgggcgc gtgctgctgg agacccattg cctggactgg
    17161 cccacgcacc tgggcgccga ggggggctac ggtaaaatcg tgcgcgtgga actactgcac
    17221 aaactgcacg acgagctgaa atacgacagt cttgacgccc tcaccgccgg catcgcccgg
    17281 gactgcgagg acgcgcgcgc cttctttgcc acggcccacg ccgagacccg tcgccagacc
    17341 acgcgcgacc gaatttgacg gggccgcctt cgccccgccc ctcgccttag ctgttgcacc
    17401 gcccttccag gcccagcgcc tggaacgccc ttgatttgaa ggtttccctc ccatgtccga
    17461 gaacaaaacg cccaagcccg aagcgtccgc ctaccgcgcc acgctgaaca tgcccgacac
    17521 gcccttcccg atgcgcggcg acctgcccaa acgcgagccg caatgggcca aggactggag
    17581 cgagcaaggc gtgtacaaaa agctgcgcga cgcgcgacgc gatgcgccca agttcatcct
    17641 gcacgacggc ccgccgtacg ccaacggcca gatccacatc ggccacgcga tgaacaagat
    17701 cttgaaggac atgatcgtca agagccgcca gctggagggt tttgacgcgc tctacacccc
    17761 cggctgggac tgccacggcc tgccgatcga gaacgccatc gagaaaaaac acggccgcaa
    17821 cctgccgcgc gacgagatgc aggcgcgcgg ccgcgccttt gccaccgagc agatcgcgca
    17881 gcagatggcc gacttccagc gcctgggcgt gctgggcgaa tgggacaacc cctaccagac
    17941 catggcctac gccagcgagg cgggcgagct gcgcgcgctg aaaaaagtga tggagcgcgg
    18001 cttcgtctac cgcggcctca agcccgtgta ctggtgcttt gactgcgcca gttcgctcgc
    18061 cgaattcgag atcgagtacc aggacaagca aagccagacg ctggacgtga tgttcccggc
    18121 ggccgacccg gcgcgcctgg ctgcagcctt cggcctgccc gcgctgacca aagaagcctt
    18181 catcgtcatc tggaccacca ccgcctggac gatccccgcc aaccaggcgc tgaacgtcaa
    18241 ccccgagctc gaatacagcc tggtcgatac cgagcgcggc ctgctcctcg tcgccagtgc
    18301 gctggtcgag aaatgcctgg agcgctggaa gctgcaaggc caggtgctcg ccacggccct
    18361 ggggaaaaag ctcgatttga tgcccttcaa gcaccccttg gcccatgtgg acaaggcctt
    18421 tgaccgcatc tccccggtgt acctggccga ctacgccacg gccgaggacg gcaccggcat
    18481 cgtgcacagc gcgccggcct atggcgtgga cgacttcaac tcctgcgtgg cccatggcct
    18541 gaaatacgag gacatcctga accccgtgca gggcaacggc gtgtacgcgc cagagctggg
    18601 gatgttcggc ggccagcata tctggaaggc cgtgccggtg atcatcgacg cgctgaaggt
    18661 cgccgggcgc ctgctggaca gcatcaccat cagccacagc tacccgcatt gctggcgcca
    18721 caagaccccg gtgatctacc gcgccgccgc gcagtggttc gtgcgcatgg acgagggcga
    18781 tggcgtgttc accgacccaa aacaaaaacc cgcacaaacc ctgcgccaga ttgcgttggc
    18841 tgctatcgaa aacacacact tctaccccga gaacggcaag gcccgcctgc gcgacatgat
    18901 cgccgggcgg cccgactggt gcatctcgcg ccagcgcagc tggggcgtgc ccctgccctt
    18961 cttcctgcat gtggactcgg gcgaactgca cccgcgcacc atggagatca tggaccaggc
    19021 cgccgagatc gttgaaaagg gcggcatcga ggcctggagc cgcgccactg cagaagaaat
    19081 tctcggtgcc gaggacgcca agctctatac caagagcacc gacatcctcg aagtctggtt
    19141 cgactcgggc tccaccttct ggcatgtgct gcgcggcacg cacgccggca tgcaccacga
    19201 cgaaggcccc gaggccgacc tgtacctgga aggccacgac cagcaccgcg gctggttcca
    19261 cagctcgctg ctgctggcca gcgccatctt tggccgcgcg ccgtaccggg gcctgctcac
    19321 gcacggcttc acggtcgatg ggcaaggcaa gaagatgagc aagtcgctgg gcaacaccgt
    19381 ctcgccgcag gaggtcagcg gcaagctggg cgccgagatc atccgcctgt ggtgcgccgc
    19441 caccgactac tcgggcgacc tcgccatcga cgacaagatc ctggcccgcg tggtggacag
    19501 ctaccgccgc atccgcaaca cgctgcgctt cctgctggcc aacgtgagcg atttcgaccc
    19561 cgccaaggac agcgtgccgt ttggcgacat gctggagatc gaccgctacg cactctcccg
    19621 cgctgccgag ttccaggcca acttgctcgc gcactacaag gtctatgagt tccaccccgt
    19681 ggtcgcaaag ctgcagctgt actgctcgga agacctgggc ggcttctacc tcgacgtgct
    19741 caaggaccgg ctgtacacca ccgcgccaaa gagcctggcg cgccgcagcg cgcagacggc
    19801 gctgtaccag atcacgcacg ccatgctgcg ctggatggca cccttcctgt cgtttacggc
    19861 ggaagaggcg tggaaggtgt ttggcgactc cgaatccatc tacctggaga cctaccagac
    19921 catccccgcc gccgacgaag gcctgctggc caagtgggcg cgcctgcgcg agatccgcga
    19981 cgcggtgaac aaggagatcg aggtgctgcg cgccgacggc caggtgggct cgtcgctgca
    20041 ggccaacgtc acgctcacgg ccgcgcccga agaccacgcc ctgctggcca gcctgggcga
    20101 tgacctgaag ttcgtgttca tcacatccgc tattgaattg gtagctggta gcgctttaca
    20161 gataagcgtt agagccagtt cttatgccaa gtgcgaacgc tgctggcact accgcagcga
    20221 cgtggggcag gatgccgcgc accccacgct ctgcggccgc tgcaccagca acctgcacgg
    20281 cgacggcgaa agccgggtgc acgcctgatg cccgccgcgc gttcgggccc cagcatgtgg
    20341 ccctggctgg cctgggccgt ggtcttgctg atagccgacc aagtgaccaa gaccctgatc
    20401 ctgaacaact accgcctggg cgactctacg ttcatcaccg ggttcttcaa catcgtgcgg
    20461 gcgcacaaca cgggggcggc gttctcgttc ctggcggacg cgggtggctg gcagcgctgg
    20521 ctattcaccg gcatcggcgc ggtggccacg atcttcatcg tgtggcagct gcgcgcgcac
    20581 cctgggcaaa agctgttttg ctttgcgctg gccagcatcc tgggcggtgc ggtgggcaac
    20641 gtggtggacc gactgatgca cggctacgtg gtggactttc tggactttca ctggaatttt
    20701 ctgtccggca tgttcccggg cgggcatttc cctgcgttca acctggccga cacggccatc
    20761 agcgtggggg ccgcctgcct gatcctggac gaactcctgc gcgtgcggcg cgagcgctga
    20821 ccgcgccccg cccgcgccaa ccccatgcca acggctcgcc acgcgcgggc cgttggcatt
    20881 gtggcggacg cagcaggggc atccgcaccg ctatggaatc aggagtatgc tcacgcgtca
    20941 gcttgatgac agccctgcca agggtgctgc acaggccata aaaaggaaga aacaaccgtt
    21001 ttccatgaag catctcttga acctgctggc tgccgtgtcg ctgctgatct ggggcacgta
    21061 cctggtgcgt accggcatcc tgagtgtgtt tggcgccaac ctgcgccagc tgattgcccg
    21121 cagcgtcagc agccgcttca ccgccgtgct gtccggcatc ggggtaacgg ccgtggtgca
    21181 gtccagcacg gccacggcac tcatcgtgtc ctcgttcgtg ggccaggggc tggtgggcct
    21241 gcctgccgcg ctggcggtga tgctgggcgc cgacgtgggc tccagcgtga tggcggtggt
    21301 gttctccctg gacctgtcgt ggctgtcgcc gctgttcatc tttgcaggcg tggtgctttt
    21361 caccgcgcgc aatggcacgg cggtgggccg cgtggggcgg gtgctgattg gcctggggct
    21421 gatgctgctg gcgctgcgcc tgatcaccga gtccaccatc gtgctcaccc agtcgcccac
    21481 ggtgcgaacg ctgctctcgg cgctcaccag cgacctgctg ctggagcttt tcaccggcgc
    21541 gctgctggcc atcgtggcct attccagcct ggccacggtg ctgctcacgg cggcgctggc
    21601 gggctcgggc ggcattccgc ccgacgtggc gctgggcctg gtgctgggtg ccaacctggg
    21661 cagcggcgtg ctggccgttc tgaccacgct caaggccaac gtgcagacgc gccaggtgcc
    21721 gctgggcaac ctgttcttca agaccattgg cgtggtcatc atgacgccgc tgaccggcct
    21781 gtggctgcag catgtgcgcc cctacgtgcc cgaaaccgcc acgctggtcg tgctgttcca
    21841 cctggccttc aacatggtgg tgggtttgct gtgcatcagc ctcacgggca cggtggcccg
    21901 cctggtgcag cgcctgctgc cgcaggacag cgccccaacg gtgggcgcgc ggctccagca
    21961 cctggaccct tccgcgctgg ccacgccctc gctggccatc tcctgcgccg cgcgcgaggc
    22021 gctgcaccag gcggatgtgg tcgagtccat gctgctgggc ctgcacaagg tgatccgcac
    22081 cgacgacctg cagctggccg aagacctgcg caagctggac gaccaggtgg acagcctgta
    22141 ctcggccatc aagtactaca tgaccaagat ctcgcgctcc gagctgaacg aggtggaagg
    22201 ccagcgctgg acggaaatca tcagcttcac catcaacatg gagcagatcg gcgacatcat
    22261 cgagcgcgtg ctcatcgaca tcgaggacaa gaagatcagg aagagccgcc agttttccga
    22321 ggccggcatg gaggaaatca acgagctgca cgcccgcctg atcagcaatc tgcggctggc
    22381 gatgagcgtg ttcctgaacg gcagcgtgcg cgacgcccag aagttgcttg aagaaaaagc
    22441 gcgctttcgc gacctggagc atgcctactc ggtcacgcac ctggcgcggc tgtcggacaa
    22501 cacggtgcag agcatcgaga ccagctcgct gcacattgat ctgatcagcg atctgaaacg
    22561 catcaactcg ctgctgtgct cgttggccta ccccatcctc gaatccgccg gggcgctggc
    22621 gcccagccgg ctcaagccgc tgcaggacca gggctgagcg cccacccgca catcacaaac
    22681 ccaggaacgc ggcgatcacc aggcccgcct ccagcgtgaa gcggccttgc gccagccact
    22741 ggcgcactgc ctgcggcgcc agcaggtcga agcgctcgac ctcgccatcc tggttgtccg
    22801 gtgccatgcc ctcgggcact tcggcgcaaa accagtcgat gcgctcgcgc atgtagcccg
    22861 cgccgccgcc ttcgcggctc tcgcggccga agtccacatg gccgccgtgg cgcaggccgt
    22921 gcagcgcggc cacccgcagg cccgcctctt cccaggtctc gcgcgccagg gcttcgtgca
    22981 gcgtgtcggc ggccgacacc atgccgccca tcagcgtgtc ccactggccg gggttgttgg
    23041 gcttggtcat tgagcgctgt tgcacccaca tgcggccgtc gggcgccagg cccaccaggt
    23101 gcaccgcgcg cgtggccagg cccagcacgc gcacggcgcc gcgctccacg gtggcgatca
    23161 ccgcgcctgc cgcgttgcac acggccagct gctcgttgcg ccagggaccg cacaggtcgg
    23221 cgtcgcgcaa ggcggcggcc agggtgttga gggcgtcggt ggcggcctct ggcggcacat
    23281 ccagctgcca ggcgggtgcg ccaccgtact cctgattcaa tagctggtag cgcttatcca
    23341 gcaagcgctg gaggctgatt ttgtttaaaa aatcgggcgc caccgagccc actacctgcc
    23401 ccgccaccac cagcgtctgg cgtggctgcg cgggcggctg ctgggcggcg cggcgcgcag
    23461 cggccagcca gccatccaag ccgggtgccg tcacagggtg aggatggtcg agccggtggt
    23521 cttgcgcgcc tccaggtcgc ggtgcgcctg ctgcacctcg gccaggggat agcgctgggc
    23581 gatgtggatc ttgacctgcc cgctggccac cacggcaaac aggtcgtcgg ccatggcctg
    23641 cgtggcctcg cgcgtggcga tgtgggtgaa cagcgtctgg cgcgtgacgt agagcgagcc
    23701 tttggcgccc aggctgcccg gcgcgaacgg cggcacgggg ccggaggcgt tgccaaagct
    23761 ggccatgagg ccgaaggggc gcaggcagtc gagcgacttg tcccaggtgt ccttgcccac
    23821 cgagtcgtac accaccttca cgcccttgcc gccggtgatc tccttcacgc gcgcggcgaa
    23881 gtcctcggtg ttgtagttga tggcatgcgc cgcgccgttg tcccgcacca gctggcactt
    23941 ggcgtcgctg cccgccgtgc caatgagctg caggcccagc gccttggccc actgcgtggc
    24001 gatcaggccc acaccgcccg cggcggcgtg gaacagcacg tggtcgcccg gctgcaggcc
    24061 ctccacgggc agcgttttct tgagcaggta ctgggcggtg aggcccttga gcatcatggc
    24121 cgcgccggtc tcgaagctga tggcgtcggg cagcttgcac acgcatttgg cgggcatcac
    24181 gcgcgcctcg caataggcac cgggcggctg gctggcatag gcggcgcggt cgcccacctt
    24241 caggtgcgtg acgccttcgc ccacggcttc gaccacgccg gcggcctcca tgccgatggt
    24301 ggcgggcagg ttaagcgggt acagacccgt gcggtggtac acatcgatga agttcaggcc
    24361 gatggcgtgg tgccggatgc ggatttcacc cggcccgggc tcacccacgg ccacgtcaac
    24421 caggtgaagt tcttcggcgc caccgggctg gcggatctgg acggcaaggc tcatgaaaac
    24481 ggtctcctgt aatggatgcg acaagggggc tgtcatcctg ccatcaatct gcaagcctcg
    24541 ctggggcccc accgttacag tgacggcatg actcaacgac tgacgcccgg cacggtggcg
    24601 ctgctggtga cggcgccgct gctgtgggcc ggcaatgccg tggtgggccg cctggttcat
    24661 gacctggtgc cgcccatcac cctcaatttt ttgcgctggg cgctggccct ggcactgctg
    24721 ttgccgctca cgcaccgcgt gctgcgcccg gacagcccgc tgtggccgca ctggcggcgc
    24781 tatgcgctgc tggggctgct gggggtgggc ctgtacaacg cactgcaata cttggcgctg
    24841 cagacctcca cacccatcaa cgtgacgctg gtgggctcca gcatgccggt gtggatgctg
    24901 gcggtgggcg cgctgttttt tggcgtagcc gtcacgcgtc ggcagctggc gggcgcggcc
    24961 ctgtcaatga tcggcgtgct caccgtgctc agccggggcg agtggagcca gctgctggcg
    25021 ctgcgcctgg tggcgggcga tctgttcatg ctgctggcca cgatttcatg gtcgttctac
    25081 agctggctgc tggtgcgcac cagcgagccc gccagcgtgc gcggcgactg ggcagccttc
    25141 ctgaccgggc aaatggtgtt tggcctggcc tggtcgggcg tctttgccag cgccgagtgg
    25201 gccacggggc acacgcacat tgcatggggc tggccgctgg tggcggcgct gctgttcatc
    25261 gccatcggtc cggcgctggt ggcctaccgc tgctggggca tcggggtgca gcgggccggg
    25321 ccggcggtgg ccgggttctt ttccaacctg acgccgctgt ttgcggccct gctgtcggcc
    25381 gcgtttctgg gcgaagcacc gagcggctac cacgccgtgg cgtttgcgct gatcgtgggc
    25441 ggaattgtgg tgtcgtcgcg gcgctgaagg ttttttaatc aaaacagccg cccgcgcttt
    25501 gtggacaagc gcaagcagct atcaaaaact aagcgccatc acacagcgcc atgcaacgcc
    25561 gtcagaacgt gccggggtag gcgccgccgt cggccagcac attctggccc gtcatgtaac
    25621 ccgcatgcac gctgcacagg aacgcgcaga tggcgccaaa ctcttcgggg ttgccaaagc
    25681 ggccggccgg gatctgcgcc tgctgtgcac gtcgcacgtc gtccaggctt ttgccggact
    25741 tggtggccgc agcgcccagc gtggtggcaa ggcggtcggt gtcgaacttg cccggcagca
    25801 ggttgttcag ggtgacgccc tgcgctgcca gcttgctgcg cgcggcgcct gccacaaacc
    25861 cggt
//
LOCUS       DAOMTQ010000027        24816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_212_length_24816_cov_26.336012, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000027 DAOMTQ010000000
VERSION     DAOMTQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 24816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24816
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212_length_24816_cov_26.336012"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PK518_04480"
     CDS             <1..518
                     /locus_tag="PK518_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM48199.1"
                     /translation="RLRAASDRTPIIMLTAKGEDVDRIVGLEVGADDYLGKPFNPREL
                     LARIHAVLRRRPPQEAPGAPSGDNEVVTFGPFTFDMGTRALQKNGEELPLTTGEFAML
                     KALVRHPRQPLSREKLALLARGREFEPFDRSLDVQISRLRKLVEVDAAAPRYIQTVWG
                     VGYVFVPDGTS"
     gene            608..2107
                     /locus_tag="PK518_04485"
     CDS             608..2107
                     /locus_tag="PK518_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM48200.1"
                     /translation="MSATHDVPPDATSPAPLEASTRRGPPRSRVGLNLFWRTFFLLGL
                     LLVGCMLAWLQTLRALELEPRTLQSAQQIASMVNLSRAALVHSDAIARVSLLKTMADQ
                     EGMRILPREPGDKFQLLEPSALGQRLTEELSRRLGPGTIVASSVNGEAGLWVGFTING
                     DPNWLLMDRSRLTAAGGKTWLIWLSIVGALSLAGAAIIARLINRPLKQLSDAANRVRD
                     GDFAAGALDEEAVTSEIREVNIGFNRMAQKLAKLEQDRAVMLAGISHDLRTPLARLRL
                     ETEMSVNDDVAREHMVADIVQLDATIDKFLDYARPDTVALNPVNLHGVVSSCVYAVQD
                     HRELQITMNVPEDLIVMADEVELARVISNLLENARRYGKNRETGITTVDIAAKAREKW
                     VLVKLRDHGPGAPSEQLANLTKPFFRGDAARTAAAGAGLGLSIVDKTVQRMGGIFALA
                     NSASGGLVAHIQLQRATDLEDGQSPEQRLQRPQVKRHLPPKSRLPKRPV"
     gene            complement(2118..2597)
                     /gene="ispF"
                     /locus_tag="PK518_04490"
     CDS             complement(2118..2597)
                     /gene="ispF"
                     /locus_tag="PK518_04490"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRM48201.1"
                     /translation="MDFRIGEGWDVHALVPGRRLVLGGVDIPHGMGLLGHSDADVLLH
                     AITDALLGAAALGDIGSHFPDSDARFKGADSEVLLTEAARRVRDKGYAIANVDSTVVA
                     QAPRLAAHIPAMRERIAGALGLQLDQVNVKAKTAERLGPVGQGLAMEARAVVLLHRH"
     gene            complement(2606..3448)
                     /gene="ispD"
                     /locus_tag="PK518_04495"
     CDS             complement(2606..3448)
                     /gene="ispD"
                     /locus_tag="PK518_04495"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRM48202.1"
                     /translation="MTDLLPNHNPARHVPGRFWALLPCAGTGQRALRVQPGAGAVLPK
                     QYQRVAGQPMVLHTLAAFAGVARLAGTLVAVAPGDDFFSSHAHPAFFALPCGGATRAD
                     TVLGGLKALQARGAQDEDWVLVHDAARCLVTTAQIDALIDACQGDSVGGLLAHKLADT
                     LKTASDGPGGVRVAATVDRSDKWLAQTPQMFRLGVLLRALEQVGAAATDEASAIEAMG
                     LRPRLVPGGAQNFKVTYPEDFALAEAVLAQRLHGGTLDRFGGQRGLAASEAINPFFAR
                     ATQR"
     gene            3529..7002
                     /gene="mfd"
                     /locus_tag="PK518_04500"
     CDS             3529..7002
                     /gene="mfd"
                     /locus_tag="PK518_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRM48203.1"
                     /translation="MQLPKLSPGKRFTLPRPVGSADALLLARLGEREKAAGRVTAIVT
                     ADAADAQRLIDEMAFFAPELRCALFPDWETLPYDSFSPHQDLISERLATLWRISQGEA
                     DVVLVPATTALYRLAPPAFLAGYTFHFKVKQKLDEAKLRAQLTLAGYQHVSQVVGHGE
                     YAVRGGLIDLFPMGSLQPYRVDLFDDEIDSIRTFDPDSQRSLYPVPEVRLLPGREFPM
                     DEAARGKFRQRWRELLEGDPTRSRIYKDMGNGVATAGIEYYLPLFFDDTATVFDYLGE
                     QATVVLHGDLETAFQRFWQDTRERYRLIQGDPDHPTLPPEALFLSAEQFYGQAKPHAQ
                     LSLRPGVEDVQDNASFQKLPDLSVVRGADDPLARLQKHIASSAHRVLLLAESDGRRES
                     LLDFLRASGLNPPAFDSLAEFQQTGAERVGIATAALATGFAWVEDGLDLVTETELFAT
                     GSTTRRRKKQEQASDVEALIKDLSELKVGDPVVHSQHGIGRYRGLIHMDMGQKNPDGS
                     PALQEFLHLEYADKAVLYVPVSQLQLIGRYTGVSADEAPLHKLGSGQWEKARRKAASQ
                     VRDAAAELLNIYARRAARQGHAFRYSPQDYEQFANDFGFEETADQNAAIHAVVQDMIS
                     PRPMDRLVCGDVGFGKTEVALRACFVAVTGGKQVAFLAPTTLLAEQHFRTLSDRFAKW
                     PVKVAEVSRFRSGKEITAAVKGIADGTVDIVVGTHKLLSESTQFKNLGLLIIDEEHRF
                     GVRHKEAMKQFRAEVDVLTLTATPIPRTMGMALEGLRDLSVIATAPQRRLAIKTFVRN
                     EGQGVIREAVLRELKRGGQCYFLHNEVETIENRRQKLEEVLPEARIAVAHGQMPEREL
                     EKVMRDFVAQRYNILLCSTIIETGIDVPSANTILISRADKFGLAQLHQLRGRVGRSHH
                     QAYAYLMVPDLDGLTKQAQQRLDAIQQMEELGSGFYLAMHDLEIRGAGEVLGENQSGN
                     MLEVGFQLYNEMLAEAVRCLKAGKEPDLLAPMQAATDINLHAPALLPDDYCGDVHLRL
                     SFYKKLATARTSDQIDGLLEEIVDRFGKLPAQAQTLIDVHRLRVLSQPYGVVKVDAAP
                     GVITVTFKPQPPIDPMNIIQLIQKNKHIKLAGNEKLRIERELPEPAQRAQLVRDVLRS
                     LGQPIAAPVAA"
     gene            7010..7684
                     /locus_tag="PK518_04505"
     CDS             7010..7684
                     /locus_tag="PK518_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48204.1"
                     /translation="MFLEATIALLALVTAIFLQPWRLLASQRPLVHESHGKPSALWTP
                     LLAALVVLPWAWALPTLHHMPLQLQWSGACLVLLTLGWPLAVPVLLAVGLIAGLVSPG
                     LGWADALGLAVWQGVVPATLALLLGWLMRRLITTRAFTGARIFVYVLGRGFLGTALCI
                     FLAGTLSQSAGHMLPGVGEQLSLVARWLMAWGDAVVTGMLCAVFVAFKPEWLATWSDD
                     LYLPKP"
     gene            7716..8444
                     /gene="serB"
                     /locus_tag="PK518_04510"
     CDS             7716..8444
                     /gene="serB"
                     /locus_tag="PK518_04510"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRM48205.1"
                     /translation="MSHTPTELSPGLVVQGIKPPLALKNFKLIAFDMDSTLINIECVD
                     EIADFVGCKAEVAAITEAAMQGVITDFKDSLRQRVALLRGVTMADLERVYSERLRINP
                     GALELVRACQAAGLKTLLVSGGFTFFAHRVREQLGIDFVRANVLEVRSSTNCGELTGR
                     LVDQAWGDICDGAEKRRTLLEVASLLGIEPDECIAMGDGANDLPMMHASGLSVAYHAK
                     PAVRTQAQVAINQGGLDRLLEVLR"
     gene            8593..9378
                     /locus_tag="PK518_04515"
     CDS             8593..9378
                     /locus_tag="PK518_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM48206.1"
                     /translation="MFKQTLSALAIAALAFSAQAADVLKVAATAVPHAEILNFIKPQL
                     KAQGVELQVKEFSDYVQPNMAVEDKQLDANFFQHQPYLDSFNKDRKTSLVIVPNGKVH
                     VEPFGAYSSKIKNVKDLKDGASIAIPNDPSNGGRALILLAKQGLIELKDPKSLTPTPL
                     DITKNPKKLKFKELEAPLLPRALADVDLALINTNYAIEAKLNPTKDALFIEGSDSPYT
                     NIVAARGDRANGADIAKLMNALHTPEVKKFIQDKYKGAVVPAF"
     gene            complement(9677..11305)
                     /locus_tag="PK518_04520"
     CDS             complement(9677..11305)
                     /locus_tag="PK518_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HRM48207.1"
                     /translation="MNLSHYPALARRTGSHHGKPGAAAQRAARGVPRSLLPAGKRPAH
                     PLPDPSDWTFELIERYHAAIAATAERFGLDTYPNQLEVISAEQMMDSYASVGMPVGYR
                     HWSYGKEFLATERRYRRGHMGLAYEIVINSNPCICYLMEENTTAMQALVIAHAAYGHN
                     SFFKNNYLFGMWTDAGSIIDYLVYARDFVAQCEERHGLDTVEQWLDSCHALANLGVDR
                     YHRPSKKSLARERAEREQREAYAQQQVNVLWRTLPARPGKDSAAQANERFPKEPEENI
                     LYFIEKNAPLLEPWQREIVRIVRKIAQYFYPQRQTQVMNEGWATFWHYTLLSTLYDEG
                     WLTDGVMIEWLSSHTNVIYQPPAGHRAYSGINPYALGFSMYQDIRRICEHPSEEDRRW
                     FPDIAGTPWLPVLHHAMQNFKDESFVGQFLSPKLMRDMRLFSIHDDASERELLVSAIH
                     DEDGYRQLRQTLSQQYDLGVREPNIQVWNVNLRGDRCLTLRHTQYQGRPLAADALEVL
                     KHVARLWGFGVQLESVSGDGELPVLLHSVPAPPV"
     gene            complement(11302..>11730)
                     /locus_tag="PK518_04525"
     CDS             complement(11302..>11730)
                     /locus_tag="PK518_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HRM48208.1"
                     /translation="HYEKIDIVFIRHHTQAAEVSEDDFFHSTESGGTVVSSALVLLDQ
                     IMRARYSSQDWNIYVAQASDGDNFTNDSGNCRSLLEERILPLVRYFAYVQVAQEEQNL
                     WQEYSQLAPRFPQFAMRKVSEPGEIYPVLRDLFKKEGVSA"
     assembly_gap    11731..11830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11831..12342)
                     /locus_tag="PK518_04530"
     CDS             complement(<11831..12342)
                     /locus_tag="PK518_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HRM48209.1"
                     /translation="MALHIIDRRLAGKNKSVGNRERFLRRYKAQIAEAVRRAVSKRDI
                     RHIEQAENITIPKKDISEPVFHHGTGGIRDTVHPGNTEHVRGDRIARPQGGAGGAGSQ
                     ASDSGEGEDDFTFTLSRDEFMQLFFEDLALPRLLRTHLGDTPLFRTRRAGYSRDGTPH
                     NLAVLRTMRGA"
     gene            complement(12420..14342)
                     /locus_tag="PK518_04535"
     CDS             complement(12420..14342)
                     /locus_tag="PK518_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HRM48210.1"
                     /translation="MDAISNFAARYVRLREEEMSIDEYLALCQRDPMAYGSAAQRMLT
                     AIGEPEMVDTRSDPHLSRLFANKVIRRYPAFAEFYGMEDAIDQVVSFFRHAAQGLEER
                     KQILYLLGPVGGGKSSIAERLKYLMQKVPFYALKGSPVNESPLGLFDVAEDGPVLEEQ
                     FGIPRRYLQHVLSPWAVKRLEEYGGDIRQFRVVKRYPSILRQVAVAKTEPGDENNQDI
                     SSLVGKVDIRKLENFAQDDTDAYSYSGGLCLANQGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNYKGTEGFGAIPFDGIVLAHSNESEWKAFRNNRNNEAFLDRIYIVKVPYCLRVSEE
                     IKIYEKLIRESSLAQAICAPGTLKMMAQFSVLTRLKEPENSSIFSKMQVYDGESLKDT
                     DPRAKSYQEYRDYAGVDEGMSGISTRFAFKILSKVFNYDSTEVAANPVHLMYVLERQI
                     EREQFPAELETKYTGYIKEYLSPRYAEFIGKEIQTAYLESYSEYGQNIFDRYVTYADY
                     WIQDSEYRDTDTGEVFDRSALNAELEKIEKPAGIANSKDFRNEIVNFVLRARANNQGK
                     NPSWTSYEKLRLVIEKKMFSNTEELLPVISFNAKASAEDARKHEDFVTRMESKGYTPK
                     QVRLLCEWYLRVRKSS"
     gene            complement(14499..15131)
                     /locus_tag="PK518_04540"
     CDS             complement(14499..15131)
                     /locus_tag="PK518_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRM48211.1"
                     /translation="MFDAAVLSSSWLTGFTVCLSLIASIGAQNLYVLRQAVQGEHVRA
                     CVAWCVASDALLVALGVAGMARLLAGAPTLALWLRLGGALFLLGYGLLAWQRALFAAD
                     AGVRAGGARVARGWLGVVGGLAVITLLNPHVYLDTVLLIGSIGARHEGALKWIFVLGA
                     ACASLTWFALLAWAGRRLQGLFARPRAWRMLDGLTGAMMLLLAWWVWQGT"
     gene            15202..16107
                     /locus_tag="PK518_04545"
     CDS             15202..16107
                     /locus_tag="PK518_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator ArgP"
                     /protein_id="HRM48212.1"
                     /translation="MLDYTSLEALATVLHEGSFERAARRLHVTPSAVSQRVKQLEERL
                     GQVLVQRGSPCTGTEAGQRLCLHLEQVALLEHQLRRSHPGLLPDAPAPTLKLAVNADS
                     LSTWFMDAMAAFTAGGPELLELRLDDQEHTAERLRRGEVVAAVTASGASIAGCNSWPL
                     GRMRYVAAASPGFIARHFAQGVSAAALAQAPVMVYDRKDRLPQQWLQAHGLATRTHAP
                     QHFLPSNFGYVRACEVGMGWGIHPTGLIGPQLAHGSLVQLLPGMALDMPLFWTHPRSA
                     QAALERLTHCVMAAARQWLEPENAI"
     gene            complement(16163..16645)
                     /locus_tag="PK518_04550"
     CDS             complement(16163..16645)
                     /locus_tag="PK518_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM48213.1"
                     /translation="MRLTQWTDYALRVLMYCAACQEREQPVTISEVADAHGISRSHLT
                     KIVQQLAMQGLLDTTRGRGGGMRLMRPAQEISVGAVVRMTETDFDMVECFNAELNQCR
                     MSQHCRLQSVLSTATRAYLAVLDGLTVADLVAPLAPGKEGGAATGQPVRWVPGLPQSA
                     "
     gene            16736..17455
                     /gene="ytfE"
                     /locus_tag="PK518_04555"
     CDS             16736..17455
                     /gene="ytfE"
                     /locus_tag="PK518_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HRM48214.1"
                     /translation="MNAIADASTIDPSQAIGQIAVQLPGATAVFRRLKLDFCCGGHVS
                     LDQAASDKGLDLQAVVHELSALQRPSELPDLGTPTELVEHIIARYHEVHRAQLPELIR
                     MARRVEAVHRDNPNTPHGLADALETMEQELLMHMHKEEAVLFPMLRAGGNSFVSQPIA
                     MMRHEHNDHGEALEHLASLTNDITPPMGACNTWRALYTGLAQLREDLINHIHLENNIL
                     FPQFEQAAQPQGCGGGGCGCA"
     gene            17452..17817
                     /locus_tag="PK518_04560"
     CDS             17452..17817
                     /locus_tag="PK518_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRM48215.1"
                     /translation="MSALDIPTREGLTDLVHGFYADVRADAQLGPLFDRAIGAHWDTH
                     LERMVEFWSTVALGTRSFKGDVFGKHMAVDGVAPEHFAVWLRLWGQHTERVFAPEVAR
                     ELQTVAHGIARNLFRGYFG"
     gene            complement(17805..18056)
                     /locus_tag="PK518_04565"
     CDS             complement(17805..18056)
                     /locus_tag="PK518_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRM48216.1"
                     /translation="MDIAGCDAQRLQLLLQREMPFGKHKGRVIADLPANYLQWFAREG
                     FPRGEIGQLLALMYEIDHNGLRGLLAPLRASQPAGRQPK"
     gene            complement(18081..19082)
                     /locus_tag="PK518_04570"
     CDS             complement(18081..19082)
                     /locus_tag="PK518_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM48217.1"
                     /translation="MQYRQLGKSPLQVSALCLGTMMFGDQTGETEAAAIVAHAREQGV
                     NYIDTADVYTQGASEAMLGRLLKGQRQDWVLATKLGNKMGDLPNRAGYSRAWMLREVE
                     ASLERLQTDHVDILYLHRDYEAMDLEEPLYAIEALLRAGKIRYWGLSNFRAWRIAELV
                     HGARRIAMPGPVVCQPYYNLLNRMPEVEILPACAHHGLGVTPYSPVARGVLTGKYLPG
                     QAPEAGTRAGRGDRRMGQTEFRQESLAIAQTLKQHADQRGVTLAQFATAWVLAHRSVS
                     SVIAGPRTLAQWQDYLPALDYRVTPEDEALIDSLVAPGHPSTPGYTDPAYPLERRRP"
     gene            complement(19317..19520)
                     /locus_tag="PK518_04575"
     CDS             complement(19317..19520)
                     /locus_tag="PK518_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HRM48218.1"
                     /translation="MSTQERLEALEVKAAFTEDLLDQLNLTIYRQQQQIDGLLRAVTE
                     LRQQRPEGGTGARSLRDELPPHY"
     gene            complement(19517..20716)
                     /locus_tag="PK518_04580"
     CDS             complement(19517..20716)
                     /locus_tag="PK518_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM48219.1"
                     /translation="MAFADRLNNVETSAIRELFKLLGKPGIISFAGGFPDSAMFDVEG
                     ISAASQRALAEEPGAALQYGATEGYQPLREQLAAFMAAKGASDVAPEQLIVTTGSQQA
                     LDLLGKTLINPGDKVIVEGPTFLATIQCFRLYGAELISAPIDGDGVKTDELERLIAEH
                     RPKFVYLIPTFGNPSGAMLSLERRKKVLELAVKYQTLVVEDDPYGDLYFGAAPPPSLL
                     ALSAQVPGSRDWLAHCGSLSKVLSPGLRVGWLMAPPELLAKATMCKQFSDAHTSTFAQ
                     ATAAQYLKAGRMPATLAHVRAVYAERAQAMSDALRAELGAAVDFVQPQGGLFVWARLT
                     GAGGAVQDGNLLAQRAIAKGVAFVPGAPFFCANPDPATLRLSFATADVAKIREGVARL
                     GQALAQP"
     gene            20807..21445
                     /gene="nth"
                     /locus_tag="PK518_04585"
     CDS             20807..21445
                     /gene="nth"
                     /locus_tag="PK518_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRM48220.1"
                     /translation="MKPRDIEPFFATLKAANPQPNTELEYTTVFELLCAVLLSAQATD
                     VGVNKATRRLFPVAGTPQAMLDLGLERLEGYIKTIGLYPTKARNLLATCRILVEQHGG
                     QVPRTREALEALPGVGRKTANVVLNVAFGQPTMAVDTHIFRVGNRTGLAPGKTPLEVE
                     KQLLRRVPREYLVDAHHWLILLGRYVCQARKPRCWECAVAPYCDYRPKTPAP"
     gene            complement(21518..22021)
                     /locus_tag="PK518_04590"
     CDS             complement(21518..22021)
                     /locus_tag="PK518_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRM48221.1"
                     /translation="MIKEVTGDILLSKADLLAHGISAFDPFDSGLALALRERWPSLVK
                     DYRHDTHSKAIGAGELWAWTGVQQGGGVRRIVNLVTQNTLGQGPSAKPGKASVENVRA
                     ALQSLARFVRQEGITSVALPRLATGVGGLAWDDVKPLITQYLGDLAVPVIVYSTYRKD
                     VQADEGL"
     gene            complement(22116..23174)
                     /gene="cobT"
                     /locus_tag="PK518_04595"
     CDS             complement(22116..23174)
                     /gene="cobT"
                     /locus_tag="PK518_04595"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRM48222.1"
                     /translation="MLNLPVIAPLNDAALTERLQFLIDHKTKPLGALGRLEALMLRLG
                     LILGSEAPALHAPQMLVCAADHGLAARGVSAYPSDVTWQMVENFLAGGAAVSVLARQH
                     GLALTVADCGVARAIPARDTAPGAPRLVSRRIAAGTQDASVGPAMTREQCAQALANGM
                     AMVRDMPGNVLLLGEMGIGNTSVASLLLARLGGVPLADCVGAGTGLDAAGIARKRAVL
                     QQALDANATATEPLAALAALGGFEVATLTGAVLQAASEHRVIVVDGFITGAAVLVAAR
                     LAPQVLQRCVFAHRSGERGHAHMLEVLRADPLIDLGLRLGEGSGAALAWPLLVSACAV
                     LREMASFESAGVNGPSGG"
     gene            complement(23400..>24816)
                     /locus_tag="PK518_04600"
     CDS             complement(23400..>24816)
                     /locus_tag="PK518_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRM48223.1"
                     /translation="AGKSWLATALCRHYSNQGLKVAPFKAQNMSNNARVVATPAGGHG
                     EIGSAQYFQALAARAAPDVRMNPLLLKPEADTHSQVVLLGQVNNELSAMPWRGRSASV
                     WPQIAAALDELRAENDVVVIEGAGSPAEINLHDSDVVNMRVARHAQARCLLVTDIDRG
                     GAFAHLYGTWALLPGDERALIQGFVLNKFRGDAALLAPAPELLQEKTGVPVVATIPMQ
                     WNHGLPEEDGVFDMATAITGGAVHTRIAVVAYPRISNLDEFQPLKNVPGVLLTWARCP
                     ADVEGADWIILPGSKATAADLAWLRAQALDAAIARHAARGGRVLGICGGLQMLGEALI
                     DTLGVDGNGPGLGLLPLVTSFEARKTVRHTRARFGEVRGAWAALAGVEVAGYEIHHGQ
                     TAQHPAMAAKGDVARELIPSLAWQNPAGNVLGLYLHGLFEDAAVLRALFGADAPTLEQ
                     VFERMADGVGWWFAPEALALD"
BASE COUNT         4124 a         8515 c         8064 g         4013 t
ORIGIN      
        1 ggcgcctgcg cgccgccagc gaccgcacgc ccatcatcat gctcaccgcc aagggggagg
       61 acgtggaccg catcgtcggc ctggaggtgg gcgccgacga ctatcttggc aaacccttca
      121 acccgcgcga gctgctggcg cgcatccacg ccgtgctgcg ccgccgcccg ccgcaggagg
      181 cgccgggcgc gccctcgggc gacaacgagg tcgtgacctt cggccccttc accttcgaca
      241 tgggcacgcg cgcgctgcag aagaatggcg aggagttgcc gctgaccacg ggcgagttcg
      301 ccatgctcaa ggccctggtg cgccacccgc gccagccgct gtcgcgcgaa aagctggccc
      361 tgctggcgcg cggccgcgag ttcgagccct tcgaccgcag cctggacgtg cagatctcgc
      421 gcctgcgcaa gctcgtcgag gtggacgcgg cagcgccacg ctacattcaa accgtgtggg
      481 gcgtgggcta tgtgttcgtg ccggacggca cgagctaagc cctccaggcc cgcaccattt
      541 ctgaacggat ggacgccagg ccccgacggg ccgcgtccgc catcttgcat tgccagccac
      601 cacagccatg agcgccaccc acgacgtgcc cccagacgcc accagcccgg cgccactgga
      661 ggcttccacc agacgaggcc ccccgcgttc acgcgtgggg ctgaatcttt tctggcgcac
      721 cttcttcctg ctggggctgc tgctggtggg ctgcatgctg gcctggctgc aaaccctgcg
      781 cgcgctggag ctggagccgc gcacgctgca gtcggcgcag cagatcgcct ccatggtcaa
      841 cctcagccgc gcggcgctgg tgcactcgga cgccattgcg cgcgtgtcgc tgctcaagac
      901 catggccgac caggagggca tgcgcatcct gccgcgcgag cccggcgaca agtttcagct
      961 gctggagcca tcggccctgg gccagcgcct gaccgaggag ctgtcgcggc gcctgggccc
     1021 gggcaccatt gtggccagca gcgtgaatgg cgaggccggg ctgtgggtgg gcttcaccat
     1081 caatggcgac cccaactggc tgctgatgga tcgctcgcgc ctcaccgccg ccggcggcaa
     1141 gacctggctg atctggctgt ccatcgtggg tgcgctgtcg ctggccggag cggccatcat
     1201 cgcgcgcctg atcaaccgcc cgctcaagca gctcagcgac gccgccaacc gcgtgcgcga
     1261 cggcgacttt gccgccggcg cgctggacga agaggccgtg accagcgaaa tccgcgaggt
     1321 caacatcggc ttcaaccgca tggcacaaaa gctcgccaag ctggagcagg accgcgccgt
     1381 aatgctggcc ggcatctcgc acgacctgcg cacgcccctg gcgcgcctgc gcctggagac
     1441 cgagatgagc gtgaatgacg acgtggcgcg cgagcatatg gtggccgaca tcgtgcagct
     1501 cgacgccacc atcgacaagt tcctggacta cgcccgcccc gataccgtgg cactcaaccc
     1561 ggtcaacctg catggcgtgg tgtcgtcctg cgtgtatgcc gtgcaggatc accgcgaact
     1621 gcagatcacc atgaacgtgc ccgaagacct gatcgtcatg gccgacgagg tggagctggc
     1681 gcgcgtgatc tccaacctgc tggaaaacgc ccgccgctat ggcaagaacc gcgagaccgg
     1741 catcaccacc gtggacattg ccgccaaggc acgtgagaaa tgggtgctgg tcaagctgcg
     1801 cgaccatggc ccgggcgcac catcggagca gctggccaac ctgaccaagc cgttcttccg
     1861 tggcgacgcg gcgcgcacgg ccgccgccgg cgcggggctg ggcctgtcca tcgtggacaa
     1921 gaccgtgcag cgcatgggtg gcatcttcgc gctggccaac tcggcctcgg gcggactggt
     1981 ggcccatatc cagctgcaac gcgccaccga cctggaggac ggccagagcc ccgagcagcg
     2041 cctgcagcgc ccgcaggtca agcgccacct gccgcccaag tcgcgcctgc ccaagaggcc
     2101 ggtttagtgg tgagtggtca gtggcggtgc agcagcacta cggcgcgcgc ttccatggcc
     2161 aggccctggc ccacggggcc aaggcgctcg gcggtcttgg ccttgacgtt cacctgatcc
     2221 agctgcagcc caagggcacc ggcgatgcgc tcgcgcatgg cggggatatg ggccgccagg
     2281 cgcggcgcct gggccaccac ggtgctgtcg acattggcaa tggcatagcc cttgtcgcgc
     2341 acgcggcgtg ccgcctcggt gagcagcacc tccgaatccg cgcccttgaa acgcgcatcg
     2401 ctgtcgggga aatgactgcc tatgtcgccc agcgccgccg cgcccagcag ggcatcggtg
     2461 atggcgtgca gcagcacgtc ggcatccgaa tgcccgagaa ggcccatgcc atggggaatg
     2521 tccaccccgc ccaacaccag ccgccgcccg ggcaccaggg catgcacgtc ccaaccctcg
     2581 ccaatcctga aatccatcgc tgttcctagc gctgcgtcgc gcgcgcaaaa aaggggttga
     2641 tcgcctcact ggcggccagg ccacgctggc caccaaacct gtccagggta ccgccatgca
     2701 ggcgctgggc cagcacggcc tcggccaagg caaagtcctc cgggtaggtg accttgaagt
     2761 tctgcgcgcc gcccggcacc aggcgcgggc gcagacccat ggcctcgatg gcgctggcct
     2821 cgtcggtggc ggcagcgccc acctgctcca gcgcgcgcag cagcacaccc aggcggaaca
     2881 tctgcggcgt ctgcgccagc catttgtcgc tgcggtccac agtggcggcc acgcgcacgc
     2941 cgcccgggcc gtcgctggcg gtcttcaggg tgtcggccag cttgtgcgcc agcaggccac
     3001 caacgctgtc accctggcaa gcgtcgataa gcgcgtcaat ctgcgccgtg gtcaccaggc
     3061 agcgcgccgc gtcgtgcacc agcacccagt cttcgtcctg cgcgccgcgc gcctgcagcg
     3121 ccttcaagcc gcccagcacc gtgtcggcgc gcgtggcgcc gccgcagggc agggcgaaga
     3181 aggccgggtg cgcatggctg ctgaagaaat catcgcccgg cgccacggcc accagcgtgc
     3241 cggccagccg ggccacgccg gcaaaggccg ccagcgtgtg cagcaccatg ggctgaccgg
     3301 cgaccctttg gtactgcttg ggcaggaccg cgcccgcgcc aggctgcacg cgcagggcgc
     3361 gctgacccgt gcccgcgcag ggcagcagcg cccagaagcg cccaggcaca tggcgggcag
     3421 ggttgtgatt gggcagcagg tcggtcatgg gtgggcattc tagaatcgcc gcttcacgcc
     3481 ccacgcctgc ctgcgcgcac agcgcagcca tccaggcaac cgtcacgcat gcagctgccc
     3541 aaactctccc ccggaaaacg cttcaccctg ccccgccccg tgggtagcgc cgacgccctg
     3601 ttgctggccc gcctgggcga acgcgaaaag gccgccggcc gcgtcaccgc catcgtcacg
     3661 gcggacgccg ccgatgccca gcgcctgatc gatgaaatgg cgttttttgc gccagagctg
     3721 cgctgcgcgc tgtttcccga ctgggaaacc ctgccctacg acagcttctc gccgcaccag
     3781 gacttgatca gcgagcgcct ggcgacgctg tggcgcatat cccaaggcga ggccgacgtg
     3841 gtactggtgc ccgccaccac ggcgctgtac cgcctggcgc cgccggcctt cctggccggc
     3901 tacaccttcc acttcaaggt caagcaaaag ctggacgaag ccaaactgcg cgcgcaactg
     3961 acgctggccg gctaccagca tgtatctcaa gtcgtgggcc atggcgaata cgcggtgcgc
     4021 ggcgggctca ttgacctgtt ccccatgggc tcgctgcagc cctatcgcgt ggatctgttc
     4081 gacgacgaga tcgactccat ccgcaccttc gaccccgaca gccagcgcag cctctacccc
     4141 gtgcccgagg tgcgcctgct gcccgggcgc gaatttccca tggacgaggc ggcgcggggc
     4201 aagttccgcc agcgctggcg cgagctgctg gagggcgacc ccacgcgcag ccgcatctac
     4261 aaggacatgg gcaacggcgt ggccaccgcc ggtatcgagt actacctgcc gctgttcttc
     4321 gacgacacgg ccacggtgtt cgactacctt ggcgaacagg ccacggtggt gctgcacggc
     4381 gacctggaga cggcctttca gcgcttctgg caggacacgc gcgagcgcta ccgcctgatc
     4441 cagggcgacc cggaccaccc cacgctgccg cccgaggcgc tgtttctgtc cgccgagcag
     4501 ttctacggcc aggccaagcc gcatgcccag ctgtcgctgc gcccgggtgt ggaggacgtg
     4561 caggacaacg ccagcttcca gaagctgccc gacctgtccg tggtgcgcgg cgccgatgac
     4621 ccgctcgcca gactgcaaaa acatatagca tccagcgcgc accgggtctt gcttttggcc
     4681 gaatccgatg gcagacgcga gagcctgctg gactttctgc gcgcatcggg cctgaacccg
     4741 ccggccttcg actcgctggc cgaatttcaa cagacgggcg ccgagcgcgt gggcattgcc
     4801 acggcggcgc tggccaccgg ctttgcctgg gtcgaagacg gcctggatct cgtcaccgag
     4861 accgagctgt tcgccaccgg cagcacgacg cgccggcgca agaagcagga gcaggccagc
     4921 gatgtcgagg cgctgatcaa ggatctgtcc gagctcaagg tgggcgaccc ggtggtccac
     4981 agccagcatg gcataggccg ctaccgcggc ctgatccaca tggatatggg gcagaaaaac
     5041 cccgacggca gcccggccct gcaggaattc ctgcacctgg aatacgccga caaggccgtg
     5101 ctctacgtgc ccgtgagcca gctgcagctg atcggccgct acaccggcgt ctcggccgac
     5161 gaggcgccgc tgcacaagct gggcagcggc cagtgggaaa aggccaggcg caaggcggca
     5221 agccaagtgc gcgatgccgc ggccgagctg ctgaacatct acgcccgccg tgccgcgcgc
     5281 cagggccatg ccttccgtta cagcccgcag gactacgagc agtttgccaa cgacttcggg
     5341 ttcgaggaga ccgccgacca gaacgccgcc atccacgccg tggtgcagga catgatcagc
     5401 ccgcgcccca tggaccgcct cgtctgcggc gacgtgggct ttggcaagac cgaggtggct
     5461 ctgcgcgcct gcttcgtcgc ggtgacgggt ggcaagcagg tggcctttct ggcgccgacc
     5521 acgctgctcg ccgagcagca tttccgcacg ctgtccgacc gttttgccaa atggccggtg
     5581 aaggtggccg aggtctcgcg ctttcgctcg ggcaaggaga tcacggccgc cgtgaaaggc
     5641 attgccgacg gcacggtgga catcgtcgtc ggcacacaca agctattgag cgaatcgacc
     5701 cagttcaaga acctgggcct tttgatcatc gacgaggagc accgctttgg cgtgcgccac
     5761 aaggaggcga tgaagcagtt tcgcgcagag gtggacgtgt tgaccctcac cgccaccccg
     5821 atcccgcgca ccatgggcat ggcgctggag ggcctgcgcg acctgtcggt gattgccacc
     5881 gcaccgcagc gccgcctggc catcaagacc tttgtgagga acgaaggcca gggcgtgatc
     5941 cgcgaggccg tgctgcgcga attgaagcgc ggcgggcaat gctactttct gcacaacgag
     6001 gtcgaaacca tagagaaccg gcgccagaag ctggaagaag tattgcccga ggcgcgcatc
     6061 gccgtggccc atggccagat gcccgagcgc gagctggaaa aggtgatgcg cgactttgtc
     6121 gcccagcgct acaacatcct gctgtgctcg accatcatcg agaccggcat cgacgtgcca
     6181 tcggccaaca ccatcctgat cagccgcgcc gacaagttcg gcctggcgca gctgcaccag
     6241 ttgcgcgggc gcgtggggcg cagccaccac caggcctacg cctacctgat ggtgccggac
     6301 ctggacggcc tgaccaaaca ggcgcagcag cggctcgacg ctatccagca gatggaagag
     6361 ctgggcagcg gcttctacct ggccatgcat gacctggaga tacgcggcgc cggcgaggtg
     6421 ctgggcgaga accagagcgg caacatgctg gaggtcggct ttcagctcta caacgagatg
     6481 ctggccgagg ccgtgcgttg cctgaaggcc ggcaaggagc cggacctgct cgcacccatg
     6541 caggcggcga ccgacatcaa cctgcacgcc ccggccctgc tgcccgacga ctactgcggc
     6601 gacgtgcacc tgcgcctgtc gttctacaaa aagctggcca cggcgcgcac cagcgaccag
     6661 atcgacggcc tgctggaaga gatcgtggac cgtttcggca agctgccggc gcaggcgcag
     6721 acgctgatcg acgtgcaccg cctgcgtgtc ctctcccagc cctacggcgt ggtcaaggtg
     6781 gatgcggccc ccggcgtgat caccgtcacc ttcaagcccc agccgccgat cgacccgatg
     6841 aacatcatcc aactgatcca gaagaacaag cacatcaaac tggccggcaa cgaaaaactg
     6901 cgcatagagc gtgaactgcc cgagccggcg cagcgcgccc agctggtgcg cgacgtgctg
     6961 cgcagcctgg ggcaaccgat tgcagccccc gtggcagcgt aaatacacta tgtttttaga
     7021 agctactatc gctttactgg cgctggttac ggccatattt ttgcaaccct ggcggctgct
     7081 cgccagccag cgccctctgg tgcatgaaag ccacggcaag ccctctgccc tgtggactcc
     7141 gctgctggcc gccctggtgg tcctgccctg ggcctgggcc ctgcccacgc tgcaccacat
     7201 gccgctgcag ctgcaatggt cgggcgcctg cctggtgctg ctgacgctgg gctggccgct
     7261 ggccgtgccg gtactgctgg ccgtggggct gatcgccggc ctggtctcgc ccggcctggg
     7321 ctgggccgac gccctgggcc tggccgtctg gcagggcgtg gtgccggcca cgctggcgct
     7381 gctgctgggc tggctcatgc gccgcctgat caccacgcgc gccttcaccg gcgcgcgcat
     7441 ctttgtctac gtgctggggc gcggctttct gggcacggcg ctgtgcatct tcctggccgg
     7501 caccctgtcg caatcggccg gccatatgct gccgggcgtg ggcgagcagc tgtcgctggt
     7561 ggcgcgctgg ctcatggcct ggggcgacgc cgtggtcacc ggcatgctgt gcgcggtgtt
     7621 cgtggccttc aagcccgaat ggctggcgac ctggtcggat gacctctacc tgcccaagcc
     7681 ctgaacgaca attcctttct ttaccctggc ccgctatgtc tcacaccccc accgaactct
     7741 cccccggcct tgtcgtgcag ggcatcaagc cgccgctggc gcttaagaac ttcaagctca
     7801 tcgctttcga catggactcc acgctgatca acatcgagtg cgtggacgag atcgccgact
     7861 tcgtcggctg caaggccgag gtggcagcca tcaccgaggc cgccatgcag ggcgtgatca
     7921 ccgacttcaa ggacagcctg cgccagcgcg tggcattgct ccgcggcgtg accatggccg
     7981 atctggagcg ggtgtacagc gagcgcctgc gcatcaaccc cggcgccctg gaactggtgc
     8041 gcgcctgcca ggcggccggg ctgaagaccc tgctggtctc gggcggtttc accttctttg
     8101 cccaccgcgt gcgcgaacag ctaggcatcg acttcgtgcg cgccaacgtg ctggaggtgc
     8161 gctccagcac caactgcggt gagctcaccg gccgcctggt cgatcaggcc tggggcgaca
     8221 tctgcgacgg ggccgagaag cgccgcacgc tgctggaggt ggcatcgctg ctgggcatag
     8281 agcccgatga atgcatcgcc atgggcgacg gtgccaacga cctgccgatg atgcatgcgt
     8341 ccggcctgtc cgtggcctac cacgccaagc cggcggtacg cacccaggcc caggtggcca
     8401 tcaaccaggg tgggctggac cggctgctgg aagtgttgcg ctgaacgacc acataaaaaa
     8461 ccacatcgtt atatcgatat gaatttaatt acgtcgtagc ccctggtaca aacccgtaaa
     8521 ctacgccgta cgccgcgccc acaccgaacc ggtctgggcg tttttctttg ttgccctaca
     8581 aggttgcatt cgatgttcaa acaaaccctc tcggccttgg cgatcgctgc cctggccttt
     8641 tccgcccagg ccgccgatgt gctcaaggtc gcggccaccg ccgtgccaca cgccgaaatc
     8701 ctgaacttca tcaagcccca gctcaaggcc cagggcgtcg agctgcaggt caaggaattc
     8761 agcgactacg tacagcccaa catggccgtc gaggacaagc agctggacgc caacttcttc
     8821 cagcaccagc cctacctgga cagcttcaac aaggatcgca agaccagcct ggtcatcgtg
     8881 cccaatggca aggtgcatgt ggagcccttc ggcgcctact cgagcaagat caagaacgtg
     8941 aaggatttga aggacggcgc ctccatcgcc atccccaacg acccgtccaa cggcggccgt
     9001 gccctgatcc ttctggccaa gcagggcctg atcgagctga aagaccccaa gagcctgacg
     9061 cccacgccgc tcgatatcac caagaacccg aagaagctca agttcaagga actggaagcc
     9121 ccgctgctgc cgcgcgccct ggccgacgtc gatctggcgc tgatcaacac caactacgcc
     9181 atcgaggcca agctcaatcc gaccaaggac gcgctgttca tcgaaggctc tgactcgccc
     9241 tacaccaaca tcgtcgccgc ccgcggcgac cgcgccaatg gcgccgacat cgccaagctg
     9301 atgaacgcac tgcacacccc cgaggtgaag aagttcatcc aggacaagta caagggtgcc
     9361 gtggtgccgg cgttctgacc gcgcgcccct cacccgcgcc ccactaaaaa aacgctgccc
     9421 acgggcagtg ttgttacttt tgctactatt tatatagctt tcagcgcttt accagcaagc
     9481 gctacaggcc aattcggcct gattaggcac atacctcaga ctgtgccgcg agccctcacc
     9541 ccaaccctct cccagaggga gagggagtaa agccggcgca accggtcgcc ctcgcccctt
     9601 tggggagagg gaggggtgag gggcctctgc gatatgtgcc atagtcaggc aattcggctt
     9661 gtaaaacaca tgcgtgtcaa accggcggcg ccggcacgct gtgcagcagc accggcagct
     9721 cgccgtcgcc gctgacgctt tccagctgta cgccaaagcc ccacaggcgc gccacatgct
     9781 tgagcacctc cagcgcatcg gccgccagcg ggcggccctg gtactgggtg tggcgcaggg
     9841 tcaggcagcg gtcgccgcgc aggttcacgt tccagacctg gatgttgggc tcgcgcacgc
     9901 ccaggtcgta ttgctgcgac agggtctggc gcagctggcg gtagccgtcc tcgtcgtgga
     9961 tggcgctgac cagcagttcg cgctcgctgg cgtcgtcgtg aatggagaac aggcgcatgt
    10021 cgcgcatcag cttggggctc aggaactggc cgacaaagct ttcgtccttg aagttctgca
    10081 tggcatggtg cagcacaggc agccagggcg tgccggcgat gtcggggaac cagcgccggt
    10141 cttcctcgct ggggtgttcg caaatgcgcc ggatgtcctg gtacatggaa aagcccaggg
    10201 cgtaggggtt gatgccgctg taggcgcggt gcccggcggg cggctggtag atgacgttgg
    10261 tgtgcgatga cagccattcg atcatcacgc cgtcggtcag ccagccctcg tcgtacaggg
    10321 tgctcagcag ggtgtagtgc cagaaggtgg cccagccctc gttcatcacc tgggtctggc
    10381 gctgggggta gaagtactgg gcgatcttgc gcacgatgcg cactatttct ctctgccagg
    10441 gctccagcag cggggcgttc ttctcgatga agtagaggat gttctcctcg ggctccttgg
    10501 gaaagcgctc attggcctgg gctgcactgt ccttgcccgg gcgcgccggc agggtgcgcc
    10561 agagcacgtt gacctgctgc tgggcgtagg cctcgcgctg ctcgcgctcg gcgcgctcgc
    10621 gcgccaggct tttcttggac ggacggtggt agcggtccac ccccaggttg gccagcgcgt
    10681 ggcaggagtc cagccattgc tcgacggtgt ccaggccatg gcgctcctcg cactgggcaa
    10741 cgaagtcgcg cgcatagacc aggtagtcga tgatgctgcc ggcgtccgtc cacatgccga
    10801 acaggtagtt gttcttgaag aagctgttgt gcccgtaggc cgcatgggcg atcaccaggg
    10861 cctgcatggc cgtggtgttc tcctccatca gatagcagat gcaggggttg gagttgatga
    10921 cgatctcgta cgccagcccc atgtggccgc gccggtagcg ccgctcggtg gccaggaact
    10981 ccttgccata gctccagtgg cggtagccca ccggcatgcc gacgctggcg taggagtcca
    11041 tcatctgctc ggccgagatc acctccagct ggttcggata ggtgtccagg ccaaagcgct
    11101 cggccgtggc ggcgatggcc gcgtggtagc gctctatcag ctcgaacgtc cagtcgctgg
    11161 ggtcgggcaa ggggtgcgcc ggacgcttgc ccgctggcag cagggagcgc ggcacgcccc
    11221 gcgcagcgcg ctgcgcggcg gcgcccggct tgccatggtg cgaaccggtg cggcgcgcca
    11281 gggcggggta atgcgagagg ttcatgccga caccccttcc ttcttgaaca ggtcgcgcag
    11341 cacggggtag atctccccgg gctcggacac cttgcgcatc gcgaactgcg ggaaccgcgg
    11401 ggcaagctga ctgtactcct gccacaggtt ttgctcctcc tgcgccacct gcacataggc
    11461 aaagtagcgc accaggggca ggatcctctc ctccagcagg ctgcggcagt tgccgctgtc
    11521 gttggtgaag ttgtcgccgt cgctggcctg ggcaacgtag atgttccagt cctgcgagga
    11581 gtagcgcgcg cgcatgatct gatcgagcag caccagggcg ctgctgacca cggtgccacc
    11641 gctctcggtg gagtggaaga aatcgtcctc gctgacctcg gcggcctggg tgtggtggcg
    11701 tatgaagacg atgtcgatct tttcgtagtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn gcgccacgca tggtgcgcag caccgccagg ttgtgcggcg tgccgtcgcg
    11881 gctgtagccg gcgcggcgcg tgcgaaacaa aggggtatcg cccaggtggg tgcgcagcag
    11941 gcgcggcagg gccaggtctt caaagaacag ctgcatgaac tcgtcgcggc tcagggtgaa
    12001 ggtgaagtcg tcctcgccct cgccgctgtc gctggcctgc gagcccgcgc caccggcacc
    12061 gccctggggc ctggcgatgc ggtcgccacg cacatgctcg gtgttgcccg gatgcacggt
    12121 atcgcgtatg ccacccgtgc cgtggtggaa caccggctcg ctgatgtcct tcttcgggat
    12181 ggtaatgttt tcggcctgct cgatatggcg aatatcgcgc ttggacacgg cacggcgcac
    12241 agcctcggcg atctgcgcct tgtagcggcg caggaagcgc tcgcggttgc ccaccgactt
    12301 gttcttgccg gcaagcctgc ggtcgatgat atgcagtgcc atctgttacc cctggagagg
    12361 ttgagcgttg agcgttttgc gagaagtccg cccgacgcaa aacgcacaac gccaagcgct
    12421 cagctgctct tgcgcacgcg caggtaccac tcgcacagca ggcgcacctg cttgggcgtg
    12481 tagcctttgg attccatgcg ggtgacaaag tcctcgtgct tgcgcgcatc ctcggcgctg
    12541 gccttggcgt tgaagctgat caccggcagc agctcctcgg tgttggagaa catcttcttc
    12601 tctatcacca ggcgcagctt ctcgtagctg gtccagctcg ggttcttgcc ctggttgttg
    12661 gcgcgcgcgc gcagcacgaa gttgacgatc tcgttgcgga aatccttgga gttggcgatg
    12721 cccgcgggct tctctatctt ctccagctcg gcattcaggg cgctgcggtc gaacacctcg
    12781 ccggtgtcgg tgtcgcggta ctcgctgtcc tgtatccagt agtcggcata ggtcacgtag
    12841 cggtcgaaaa tgttctggcc gtactcgctg tagctctcca ggtaggcggt ctggatctcc
    12901 ttgcctatga actcggcgta acgcggcgag agatattcct tgatgtagcc ggtgtacttg
    12961 gtctccagct cggcggggaa ctgctcgcgc tctatctgcc gctccagcac atacatcaga
    13021 tgcaccgggt tggccgccac ctcggtgctg tcgtagttga aaaccttgga caggatcttg
    13081 aaggcaaagc gcgtggagat gccactcatg ccctcgtcca cgccggcgta gtcgcggtac
    13141 tcctggtagc tcttggcgcg cgggtcggtg tccttgaggc tttcgccgtc gtagacctgc
    13201 atcttgctga agatgctgga attctcgggc tccttcaggc gcgtgagcac ggagaactgc
    13261 gccatcatct tcagcgtgcc gggcgcgcaa atggcctggg ccagagacga ttcgcggatc
    13321 agcttctcgt agatcttgat ctcttcagag acgcgcaggc agtacggcac cttgacgatg
    13381 tagatgcgat ccagaaaggc ctcgttgttg cggttgttgc gaaatgcctt ccactcgctc
    13441 tcgttgctgt gcgcgagcac gatgccgtcg aacggtatcg cgccaaagcc ctccgtgccc
    13501 ttgtagttgc cctcctgcgt ggcggtgagc agcgggtgca gcaccttgat gggcgccttg
    13561 aacatctcga cgaactccag caggccctga ttggccaggc acaggccgcc cgagtagctg
    13621 taggcgtcgg tgtcgtcctg ggcaaagttt tccagcttgc ggatgtccac cttgcccacc
    13681 aggctggaga tgtcctggtt gttctcgtca cccggctcgg tcttggcaac cgccacctgg
    13741 cgcaggatgc tggggtagcg cttgaccacg cgaaactggc ggatgtcgcc gccgtattcc
    13801 tccaggcgct tgaccgccca gggcgagagc acatgctgca ggtagcggcg cggaatgccg
    13861 aactgctcct ccagcaccgg gccatcctcg gccacgtcga acaggcccag cggcgactcg
    13921 ttcaccggcg agcccttgag ggcgtagaac ggcaccttct gcatcaggta tttgaggcgc
    13981 tcggcgatcg agctcttgcc cccgcccacc gggcccagca ggtagaggat ctgcttgcgc
    14041 tcctccagcc cctgggcggc atggcggaag aagctcacca cctggtcgat ggcgtcctcc
    14101 atgccataga actcggcaaa cgcggggtag cggcgaatga ccttgttggc gaacaggcgc
    14161 gacaggtgcg ggtcgctgcg cgtgtccacc atctcgggct cgccaatggc cgtgagcatg
    14221 cgctgtgctg cgctgccgta ggccatggga tcacgctggc acagcgcgag gtattcgtcg
    14281 atcgacatct cctcttcgcg cagacggacg taacgtgcgg caaagttgct gatggcatcc
    14341 ataagagcct cctatggcgg cacgcccacc gcggacggtg ccgcaaaact gcattacctg
    14401 accagccctg cgagatcgtt gtagcacctt gctccggctt tgaacaggtg cgtgaatccc
    14461 gcagtggcgc ctgtcccgat ggataacgat gagccgcctc aggttccctg ccagacccac
    14521 caggccagca acagcatcat ggcgcccgtc aggccatcga gcatgcgcca ggcgcgcggc
    14581 cgggcaaaca gcccctgcag gcgccggccg gcccaggcca gcaaggcaaa ccaggtcagg
    14641 ctggcgcagg ctgctcccag cacgaagatc cacttgagcg cgccctcgtg gcgcgcgcct
    14701 atgctgccga tgagcaacac cgtgtccagg tacacatggg ggttgagcag ggtgatcacc
    14761 gccaggccgc cgaccacacc cagccagccg cgcgccacgc gcgcgccgcc tgcccgcacg
    14821 ccggcatcgg ccgcaaacag cgcacgctgc caggccaaca gcccatagcc cagcagaaac
    14881 agcgcgccgc ccaggcgcag ccacagcgcc agcgtgggcg cgccggccag cagccgcgcc
    14941 atgccggcca cgcccagggc caccagcagc gcatcgctgg ccacgcacca ggccacgcag
    15001 gcgcgcacat gttcaccctg caccgcctgg cgcaacacgt agaggttttg tgcgcctatg
    15061 gaggcgatca gcgacagaca cacggtaaag cccgtgagcc aggatgaaga gaggacagca
    15121 gcatcgaaca tggcccgcat gctaggcccg ccaatggttt acgtatactt aagttgtcta
    15181 aacgatattt agcaattctg catgctggac tacaccagtc tcgaggccct ggccaccgtg
    15241 ctgcacgagg gcagcttcga gcgcgcggcg cgccgcctgc atgtcacgcc ctcagccgtg
    15301 tcgcagcgcg tgaagcagct cgaagagcgc ctgggccagg tgctggtgca gcgcggcagc
    15361 ccctgcaccg gcaccgaggc cggccagcgc ctgtgcctgc atctggagca ggtggcgctg
    15421 ctggagcacc agctgcgccg cagccacccc ggcctgctgc ccgatgcacc cgcccccacg
    15481 ctgaagctgg cggtgaacgc cgactcgctc tccacctggt tcatggatgc catggcggcc
    15541 ttcaccgccg gcggccccga gctgctggag ctgcgcctgg acgaccagga gcacacggcc
    15601 gagcgcctgc gccgtggcga ggtggtcgcc gccgtcaccg ccagcggcgc cagcattgcc
    15661 ggctgcaaca gctggccgct ggggcgcatg cgctacgtgg cggccgccag cccgggcttc
    15721 atcgcccggc atttcgccca gggtgtgtcg gccgctgccc tggcgcaggc gccggtgatg
    15781 gtctacgacc gcaaggatcg cctgccgcag cagtggctgc aggcccatgg cctggccacg
    15841 cgcacgcatg cgccgcagca tttcctgccg tccaacttcg gctatgtgcg cgcctgcgag
    15901 gtgggcatgg gctggggcat acaccccacg gggttgattg gcccgcaact ggcccatggc
    15961 agcctggtgc aactgctgcc cggcatggcc ctggacatgc ccctgttctg gacccacccg
    16021 cgcagcgccc aagcggcgct ggagcggctc acgcactgcg tgatggcggc cgcaaggcaa
    16081 tggctggagc cagagaatgc tatttaatta atagctgttt gcgcttgttg cacaagggct
    16141 gcagccgtat tcaacccatt tttcacgccg actgcggcag gccgggcacc cagcgcacgg
    16201 gctggcccgt cgccgcgccg ccttccttgc ccggagccaa cggcgccacc aggtcggcca
    16261 cggtcaggcc gtcgagtacc gccaggtagg cgcgtgtcgc cgtggacaac acgctttgca
    16321 gccggcaatg ctggctcatg cggcactggt tcagctcggc gttgaagcat tcgaccatgt
    16381 cgaagtcggt ctcggtcatg cgcaccacgg cgcccacgct gatctcctgc gccggccgca
    16441 tcaggcgcat gccgccgccg cgtccgcgcg tggtgtccag caggccctgc atggccagct
    16501 gctgcacgat ttttgtcagg tggctgcgcg atatgccgtg tgcgtcggcc acctcgctga
    16561 tggtcacggg ctgctcgcgc tcctggcagg cggcgcagta catcagcacg cgcagcgcgt
    16621 aatcggtcca ctgggtcagt cgcatggggt tcccacggtg caaggcaaaa gattcacatc
    16681 cgatgcattt tagagaataa aatattcaga ttaaatgaat ctttaggaac ccaccatgaa
    16741 cgccatcgcc gacgccagca ccattgaccc cagccaggcc ataggccaga tcgccgtgca
    16801 gctgcccggc gccactgccg tgtttcgccg tctgaagctc gatttctgct gcggcggcca
    16861 tgtcagcctg gaccaggcgg ccagcgacaa gggcctggat ctgcaggccg tggtgcatga
    16921 gctatcggcg ctgcaacgcc ccagcgagct gcccgacctg ggcacgccca cggagctggt
    16981 cgagcacatc atcgcgcgct accacgaggt gcaccgcgcc cagctgcccg agctgatccg
    17041 catggcgcgc cgcgtggagg ccgtgcaccg cgacaacccc aacaccccgc atggcctggc
    17101 cgatgcgctg gagaccatgg agcaggagct gctcatgcac atgcacaagg aagaggccgt
    17161 gctgttcccc atgctgcgcg ccggcggcaa cagcttcgtg agccagccca tcgccatgat
    17221 gcgccacgag cacaacgacc atggcgaggc gctggaacac ctggccagcc tgaccaacga
    17281 tatcaccccg cccatgggcg cctgcaacac ctggcgcgcg ctctacaccg gcctggcgca
    17341 gctgcgcgaa gacctgatca accatatcca cctggagaac aacatcttgt tcccgcaatt
    17401 cgagcaggcc gcgcaaccgc aaggctgcgg cggcggtggc tgcggctgcg catgagcgcc
    17461 ctggacatcc ccacgcgcga gggcctgacc gacctggtgc acgggttcta cgccgacgtg
    17521 cgtgccgacg cccagctggg cccgctgttc gaccgcgcca ttggcgcgca ctgggacacg
    17581 cacctggagc gcatggtgga gttctggagc accgtggcgc tgggcacgcg cagcttcaag
    17641 ggcgacgtat tcggcaagca catggccgtg gacggcgtcg cgcccgagca tttcgccgtc
    17701 tggctgcgcc tgtggggcca gcacaccgag cgcgtgttcg cccccgaggt ggcgcgcgag
    17761 ctgcagaccg tggcccacgg cattgcgcgc aacctgtttc gcggctattt cggctgacgg
    17821 cctgctggct ggctggcgcg cagcggcgcc agcagcccgc gcaggccgtt gtggtctatc
    17881 tcatacatca aggccagcag ctggccgatc tcgccgcgcg gaaagccctc gcgcgcaaac
    17941 cattgcaggt agttggccgg caggtcggca atcaccctgc ccttgtgctt gccaaacggc
    18001 atctcgcgct gcagcaatag ctgcaggcgc tgcgcgtcac agccggcgat gtccatcaca
    18061 gacctccggg gcgcagccgc tcacggccgg cgccgctcaa gcgggtaggc cggatcggta
    18121 tagccgggcg tggacgggtg gccgggcgcc accaggctgt cgatgagcgc ctcgtcctcg
    18181 ggcgttaccc gatagtccag cgccggcagg tagtcctgcc actgcgccag cgtgcgcggg
    18241 ccggcgatga cgctgctcac gctgcggtgc gccagcaccc aggcggtggc gaactgcgcc
    18301 agcgtcacgc cgcgctggtc ggcatgctgc ttgagcgttt gcgcgatggc cagcgactcc
    18361 tggcggaact cggtctggcc catgcgccgg tcgccacgcc cggcgcgcgt gccggcctcg
    18421 ggcgcctggc ccggcaggta cttgccggtc agcacaccgc gcgccaccgg gctgtagggc
    18481 gtcacgccca ggccatggtg cgcgcaggcc ggcagtatct cgacctcggg catgcggttg
    18541 agcaggttgt agtacggctg gcacaccacc ggcccaggca tggcgatgcg gcgcgcgcca
    18601 tgcaccagct cggcaatgcg ccaggcgcgg aagttggaca ggccccagta gcgaatcttg
    18661 ccggcacgca gcagcgcctc gatggcgtag agcggctcct ccagatccat ggcctcgtag
    18721 tcacggtgca ggtagaggat gtccacatgg tcggtctgca gccgctcaag gctcgcttcc
    18781 acctcgcgca gcatccaggc gcgcgagtag cccgcccggt tgggcaggtc gcccatcttg
    18841 ttgcccagct tggtggccag cacccagtcc tggcgctggc ccttgagcag ccggcccagc
    18901 atggcctcgg acgcgccctg ggtgtagacg tcggccgtgt cgatgtagtt cacgccctgc
    18961 tcgcgcgcat gggcgacgat ggcggccgcc tcggtctcgc ccgtctggtc gccaaacatc
    19021 atggtgccca ggcacagggc cgagacctgc agcgggctct tgcccaattg acggtattgc
    19081 atggagttgc tccaactatc aaaaaaagag ctgttagcgc tttattgacg ggcgctacag
    19141 gccaattttc ctgactatgt acatgtcgca gatgcccctc acccctccct ctccccaaag
    19201 gggcgagggc gaccggttgc gccggcttta ctccctctcc ctctgggaga gggtgggggt
    19261 gagggctcgc ggcccagtct gaggtatgcg cctagtcagg ccaattttct tcaaaatcaa
    19321 taatgcggcg gcagctcgtc gcgcaggctg cgtgcgccgg tgccgccctc ggggcgctgc
    19381 tggcgcagct cggtcacggc gcgcagcaac ccgtcaattt gctgctgctg gcggtagatg
    19441 gtcaggttca gctgatccag cagatcctcg gtgaaggcgg ccttgacctc cagcgcctcc
    19501 agccgctctt gcgtgctcat ggctgcgcca gcgcctggcc caggcgcgcc acgccctcgc
    19561 ggatcttcgc cacgtcggcc gtggcgaagg acaggcgcag cgtggccggg tccgggttgg
    19621 cgcaaaagaa cggcgcgccc ggcacaaagg ccacgccctt ggcgatggca cgctgggcca
    19681 gcagattgcc atcctgcacg gcgccacccg caccggtcag gcgcgcccag acgaacaggc
    19741 cgccctgggg ctgaacaaag tccacggccg cgcccagctc ggcgcgcagc gcgtcgctca
    19801 tggcctgggc gcgctcggca tacaccgcac gcacatgggc cagcgtggcc ggcatgcgcc
    19861 cggccttgag gtactgcgcg gccgtggcct gggcgaaggt gctggtgtgc gcgtcggaaa
    19921 actgcttgca catggtggcc ttggccagca actcgggcgg ggccatcagc cagcccacgc
    19981 gcaggccggg cgaaagcacc ttggacagtg acccgcaatg cgccagccaa tcacggctgc
    20041 ccggcacctg ggccgacaag gccaacaggc tggggggagg agccgcacca aagtacaggt
    20101 cgccataggg gtcgtcctcg acgaccaggg tctggtactt gacggccagc tccagcacct
    20161 tcttgcgccg ctccaggctg agcatggcgc cgctggggtt gccgaaggtg ggaatcaggt
    20221 agacgaattt ggggcggtgc tcggcaatca ggcgctccag ctcgtcggtc ttcacgccat
    20281 cgccgtcgat gggcgcgctg atcagttcgg cgccatacag gcgaaagcac tggatggtgg
    20341 cgagaaacgt cgggccttcg acgatcacct tgtcgccagg gttgatcagg gtcttgccca
    20401 gcagatccag cgcctgctgg ctgccggtgg tgacgatgag ctgctccggc gccacatcgc
    20461 tcgcgccctt ggccgccatg aaggcggcga gctgctcgcg cagcggctgg tagccctcgg
    20521 tggcgccgta ctgcagggcg gcgccgggct cctcggccag cgcacgctgg ctggccgcgc
    20581 tgataccctc cacgtcgaac atggcgctgt ccggaaaccc cccggcaaaa ctgatgatgc
    20641 cgggcttgcc cagcagcttg aaaagctcgc ggatggcgga ggtttctacg ttattcagac
    20701 ggtcggcaaa ggcaatgggc atgggtgcaa tccctgttgt gatggcaaaa cggcaggccc
    20761 gcattgtcgc caattcacat cgccgagccg gtgacaatgg cgccccatga agccgcgcga
    20821 catagaaccc ttcttcgcca ccctcaaggc cgccaacccg cagcccaaca ccgaactgga
    20881 atacaccacg gtgttcgagc tgctctgcgc cgtgctgctc tcggcccagg ccaccgacgt
    20941 gggcgtgaac aaggccacgc gccgcttgtt tccggtggcg ggcacgccgc aggccatgct
    21001 ggatctgggg ctggagcgcc tggagggcta catcaagacc ataggcctgt acccgaccaa
    21061 ggcgcgcaac ctgctggcca cctgccgcat cctggtcgag cagcatggcg gccaggtgcc
    21121 acgcacgcgc gaggcccttg aggccctgcc cggcgtgggc cgcaaaaccg ccaacgtggt
    21181 gctgaacgtg gccttcggcc agcccaccat ggcggtggac acgcatatct ttcgcgtggg
    21241 caaccgcacc ggcctggcgc cgggcaagac cccgctggag gtggaaaaac aactactgcg
    21301 gcgcgtgccg cgggagtacc tggtggacgc acaccactgg ctgatcctgc tgggccgcta
    21361 tgtctgccag gcgcgcaagc cgcgctgctg ggaatgcgcg gtggcgccgt attgcgatta
    21421 ccggcccaag acgccggcgc cctgaatacc cccttccacc aacggcctgg cccgccaggg
    21481 cgggccagga atcagcgcat cgcacgcccc ggggcgctta cagcccttcg tcggcctgca
    21541 catccttgcg ataggtgctg tagacgatca cggggacggc cagatcgccc aggtactggg
    21601 tgatcagcgg cttcacgtcg tcccacgcca ggccacccac gcccgtggcc aggcgcggca
    21661 gggccacgct ggtgatgccc tcttgccgca cgaacctggc caggctttgc agggcggcgc
    21721 gcacgttctc gacgctggcc ttgcccggct tggcactggg gccctggccc agggtgttct
    21781 gggtgaccag attcacgatg cggcgcacgc cgccgccttg ctgcacgccc gtccacgccc
    21841 acagctcgcc ggcccctatg gccttggaat gggtgtcgtg gcggtagtcc ttgaccagcg
    21901 agggccagcg ctcgcgcagc gccagggcca ggccactgtc aaaagggtca aatgccgaaa
    21961 tgccgtgggc aagcaggtct gccttgctga gcaggatatc gccggtaact tccttgatca
    22021 ttcgctgttc ctttcagtga cggatacggg taattcctat tgcccgcatc tcaaatgaac
    22081 ggtaacgcgt tttcccgccg gcaacttgat acaggtcaac cgccactcgg cccattcacc
    22141 ccggcggact cgaagctcgc catctcgcgc aacacggcgc aggccgacac caacagcggc
    22201 caggccagcg ccgcacccga gccctcgccc aggcgcaggc ccaggtcgat cagcggatcg
    22261 gcgcgcagca cctccagcat atgggcgtgg ccgcgctcgc ccgagcggtg ggcaaacacg
    22321 caacgctgca gcacctgggg cgccaggcgc gcggccacca gcaccgccgc gccggtgatg
    22381 aagccatcga ccacgatcac gcggtgctcg ctggctgcct gcagcacggc gccggtgagc
    22441 gtggcgacct cgaagccgcc cagggccgcc agcgccgcca ggggttcggt ggccgtggcg
    22501 ttggcatcca gcgcctgctg cagcacggcc cgcttgcgcg cgatgccggc cgcgtccagc
    22561 cccgtgcccg cgcccacgca gtcggccagc ggcacgccac ccaagcgcgc cagcagcagc
    22621 gaggccaccg aggtgttgcc tatgcccatc tcgcccagca gcagcacgtt gcccggcatg
    22681 tcgcgcacca tggccatgcc gttggcaagg gcctgggcgc attgctcgcg cgtcatggcc
    22741 gggcccacgc tggcgtcctg cgtgccggcg gcaatgcggc ggctgaccag gcgcggcgcg
    22801 ccgggggcgg tatcgcgggc gggaatggca cgcgccacgc cgcagtcggc caccgtcagc
    22861 gccaggccgt gctggcgcgc cagcacgctc acggcggcgc cgccggccag gaagttctcc
    22921 accatctgcc aggtcacgtc gctggggtag gccgacacgc cccgcgccgc caggccgtgg
    22981 tcggccgcgc acaccagcat ctgcggcgca tgcagcgcgg gcgcctcact gcccaggatc
    23041 aggcccaggc gcagcatcag cgcctccagc cggcccaggg cgcccagggg cttggtcttg
    23101 tggtcgatca ggaactgcag gcgctcggtc agcgctgcgt cgttcaaggg ggcgattacg
    23161 gggaggttca acattcaatt actcttaaaa caatagctat ctgcgcttga taaacaagcg
    23221 ttacaggcca atttcacctg attagacaca tgcgtcggat agccgtcatt ccggcgaatg
    23281 ccggaatcca ggtacctcgc gctggcaata accgtggatt ccggctttcg ccggaatcac
    23341 gcggtgctac tgcagcgtgc tatctggggc atgtcgccag tcaggcaatt ccattcaaat
    23401 caatccaggg ccagcgcctc gggcgcaaac caccacccta cgccgtccgc catgcgctcg
    23461 aacacctgct ccagcgtggg cgcgtcggcg ccaaacagtg cgcgcagcac ggcggcgtcc
    23521 tcgaacaggc cgtgcaggta caggcccagc acgttgccgg ccgggttctg ccaggccagg
    23581 ctggggatca gctcgcgcgc cacgtcgccc ttggcggcca tggccgggtg ctgggcggtc
    23641 tggccgtggt ggatctcgta gccggcgacc tctaccccgg ccagcgccgc ccaggcgccc
    23701 cgcacctcgc caaagcgcgc ccgggtgtgg cgcacggtct tgcgcgcctc gaagctggtc
    23761 accagcggca gcaggcccag gccagggccg ttgccgtcca cgcccagggt gtctatcagc
    23821 gcctcaccca gcatctgcag gccgccgcag atgcccagca cgcggccgcc gcgcgcggca
    23881 tgccgggcaa tcgccgcatc cagagcctgc gcgcgcagcc aggccaggtc ggcggccgtg
    23941 gccttggagc cgggcaggat gatccagtcg gcgccctcca cgtccgccgg gcagcgcgcc
    24001 caggtgagaa gcacgccggg gacgttcttc aagggctgga attcgtccag attgctgatg
    24061 cgcggatagg ccacgacggc gatgcgcgtg tgcaccgcac cgccggtgat agcggtggcc
    24121 atgtcgaata cgccgtcctc ctcgggcagg ccgtggttcc actgcatggg gatggtggcc
    24181 accaccggca cgccggtctt ctcctgcagc agctcgggcg cgggcgccag cagcgccgcg
    24241 tcgccacgga acttgttgag tacaaagccc tggatcaagg cgcgctcatc gcctggcagc
    24301 agtgcccagg tgccgtacaa atgggcaaac gcgccgccgc ggtcgatgtc ggtcaccagc
    24361 aggcagcgcg cctgggcgtg gcgcgccacg cgcatgttga ccacgtcgct gtcatgcagg
    24421 ttgatctcgg ccggcgagcc cgcgccctcg atgaccacca cgtcgttctc ggcgcgcaat
    24481 tcatccagcg ccgccgcgat ctgcggccag acgctggcgc tgcgcccgcg ccagggcatg
    24541 gccgacagct cattgttcac ctgcccgagc agcaccacct ggctgtgcgt gtcggcctcg
    24601 ggcttgagca agagcgggtt catgcgcaca tcgggcgcag cgcgcgcggc cagggcctgg
    24661 aagtactgcg cgctgccgat ctcgccgtgg ccaccggccg gcgtggcgac cacgcgcgcg
    24721 ttgttgctca tgttctgcgc cttgaacggc gccaccttca gcccctggtt gctgtagtgg
    24781 cggcacaggg ccgtggccag ccagctcttg ccggcg
//
LOCUS       DAOMTQ010000028        23355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_232_length_23355_cov_29.187597, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000028 DAOMTQ010000000
VERSION     DAOMTQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 23355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23355
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232_length_23355_cov_29.187597"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..994
                     /locus_tag="PK518_04605"
     CDS             131..994
                     /locus_tag="PK518_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRM48224.1"
                     /translation="MNTKQQLKALAGARRGALVPGAFNALSAKLIEDLGFEAIYITGA
                     GVTNMWFAMPDQGFMGLHEIAEHTARIRDAVQLPLIVDADTGFGNALNVYHTVRTLER
                     AGADCIQLEDQVHPKRCGHFSGKEVISTEEAVAKIKAAVDARRESATLIMARTDAAAT
                     HGFEAAVERAQRFAEAGADILFVEAVTQAEEVRALPQRLATPQLMNMVIGGKTPIFDA
                     EQLGELGYGLVLYANAALQGAVMGMHKALSTLRDDKQVLESSGLVTPFAERQRLVGKP
                     GWDALEEKYRG"
     gene            complement(1010..1537)
                     /locus_tag="PK518_04610"
     CDS             complement(1010..1537)
                     /locus_tag="PK518_04610"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HRM48225.1"
                     /translation="MHHDPGGKHKPQWPSGSVVQAQFSGCGRYRYSLSEVWDTNRALV
                     MFLLMNPSVAGVEHADPTLIKTGKYARAWGYGGQLVGNVHAYRVTDSRRLVEADDPVG
                     PENDARLLHMARQAERVVLAYGLPPQPLRARAAAVVQILRRAAPLQYLQLTKNGTPRH
                     PLYLKGSLLPRDFVA"
     gene            1676..1993
                     /locus_tag="PK518_04615"
     CDS             1676..1993
                     /locus_tag="PK518_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48226.1"
                     /translation="MAHTAFASVKLPSPLVEQARQAAQPMRRSVASQIEYWATLGQIV
                     EHTGLSVQEARAAIEQYEARHTPTPPASIDALTTRLVAAQTQGSLAERVRQVVQENQA
                     RAS"
     gene            2072..2605
                     /locus_tag="PK518_04620"
     CDS             2072..2605
                     /locus_tag="PK518_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48227.1"
                     /translation="MREGILGPPLEFVNADPHERAHLQHITSGEERSRAARTWADGRR
                     ATLLATRTPFATETVFSHPSKLDLIANAQRQGFTVALYVVALDDPQRLLGRVAQRVRE
                     GGHPVPPERILARYPRTMALLAQAVRQADVAYLYDAAEVRAGGPQLVAVRSAGNSTPV
                     VAPLPAWAQAMLGAQRQ"
     gene            complement(2621..3541)
                     /locus_tag="PK518_04625"
     CDS             complement(2621..3541)
                     /locus_tag="PK518_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM48228.1"
                     /translation="MLDRIHLAVIQEVERQGSLTAAADRLCVTQSALSHSMKKLEQQL
                     GTPIWLREGRSLRLTQAGRYLLELAGRVLPQLQLAEERLRQFAQGERGSLRIGMECHP
                     CYQWLLKVVSPYLAAWPDVDVDVKQRFQFGGLDALQDHEIDLLVTPDPVQRPGLHFEP
                     VFDYEQVLVVARGHALAGAAHALPRQLAQEVLIAYPVAPERLDIYTRFLLPAGVAPAR
                     HKTIETTDIMLQMVAGGRGVAALPRWLVQEYAARMDVVPVRLGPRGIAKQIHLGARVA
                     DLQTDYLQAFIALARSMSAQKAISLEATQT"
     gene            3643..5934
                     /gene="metE"
                     /locus_tag="PK518_04630"
     CDS             3643..5934
                     /gene="metE"
                     /locus_tag="PK518_04630"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRM48229.1"
                     /translation="MTTIHNLGFPRMGARRELKFALESYWRGESSRAALQEQGARLRQ
                     RHWQEQAGLDWVPVGDFSFYDQVLDMSFTLGHLPERVQGFHGDALDNYFRVARGRSAQ
                     AEAHQGCCGAVAAGEMTKWFDTNYHYIVPEFTAATQFRLDASRLLAQLAEAGAQGVPA
                     KPVLIGPVTYLALGKAKDGSNRLALLERLLPVYAELLDTLAAHGVQWVQIDEPILVTE
                     LDADWRHAFTTAYHQLKSCRVKLLLATYFGQLQDNAYLAANLPVAGLHVDAVSDPDGV
                     LPLLGLLPAHKVLSLGVVNGRNVWKTDLNATLDWLEPLAARLGERLWLAPSCSLLHVP
                     VDLAQEQRLDAELKSWLAFALQKLDELRLLGRALRDGRGAVEAELAENHAALVARRTS
                     PRVHSPAVRAAVAAITADMGRRQSPYASRAARQAALLQLPAYPTTTIGSFPQTAQIRQ
                     ARSAFKAGQIDAAAYREAMRAEIARSVRAQEELGLDVLVHGEAERNDMVEYFGEQLDG
                     YAFSQFGWVQSYGSRCVKPPILFGDIQRPRAMTVDWTRFAQSLTDRPMKGMLTGPVTL
                     LNWSFVRDDQPRAATCRQLALAMRAEVLDLERAGVRVIQIDEAALREGLPLRRAQWSQ
                     YLDWAVQAFRITANGVRDETQIHTHMCYSEFNDIMAAIAAMDADVITIETSRSDMELL
                     DAFEDFQYPNQIGPGVYDIHSPNIPAAQQMIALIKKAADRIPAERLWVNPDCGLKTRQ
                     WAEVVPALTEMVAAARALRAGSS"
     gene            6021..7457
                     /gene="pyk"
                     /locus_tag="PK518_04635"
     CDS             6021..7457
                     /gene="pyk"
                     /locus_tag="PK518_04635"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM48230.1"
                     /translation="MQVRRATKIVATLGPASSDPQLLEQMIAAGVNVVRLNFSHGKAS
                     DHVDRATMVREAARRAGREVAIMADLQGPKIRVGKFADGKVMLEPGAPFVLDASRTEP
                     GDLQGVGLDYKELPRDVKPGDTLLLNDGLIVLTVDAVRGDAVHTTVKLGGELSNNKGI
                     NKQGGGLTAPALTAKDMEDIKTAMSFQADYVAVSFPKNATDMEMARQLCNVAAAEFRH
                     RPGLIAKIERAEAIPRLEEILKVSDGIMVARGDLAVEVGNAAVPALQKKMIRMARDMD
                     KVVITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPLETVEEMA
                     KICAAAEAAEDPDRDADFDGQTLGRIDQSVAMGALFTAHHLGAKAIVAMTDSGSTALW
                     MSRNRIHIPIYALTPKVATQRRMAMYRNVRPLLMDTSADRDTALEQAENHLKMRNIVL
                     QGDLYVITCGEPMGQPGGTNMLKICRVH"
     gene            7576..8640
                     /gene="fba"
                     /locus_tag="PK518_04640"
     CDS             7576..8640
                     /gene="fba"
                     /locus_tag="PK518_04640"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914537.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRM48231.1"
                     /translation="MPLVSMRELLDHAADNGYGIPAFNVNNLEQVQAVMTAADEVGAP
                     VILQASAGARKYAGESFIKYLIAAAAEAYPHIPLVMHQDHGTSPAICQGALDLGFGSV
                     MMDGSLKEDGKTPADFDYNVRVTRQVVEMAHKVGATVEGELGCLGSLETGEAGEEDGI
                     GAVGKLSHAQMLTDPEEAAQFVKATQLDALAIAIGTSHGAYKFTRPPTGDVLAISRVK
                     EIHARIPNTHLVMHGSSSVPQDLLDIINQYGGKMKQTYGVPVKEIQEAIKHGVRKINI
                     DTDIRMAMTGAVRKFQFENPDKFDMREWMKPAREAAKAICKQRYLEFGCEGQAAKIKG
                     YTLDEMAARYAAGALRQVVN"
     gene            8855..9541
                     /locus_tag="PK518_04645"
     CDS             8855..9541
                     /locus_tag="PK518_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48232.1"
                     /translation="MPCALLTLTACAPLASQQAEYPAGRARLALPAGAWEDLGTSDQA
                     VRLLPQPDGRIALQSRVLGLRGAQQELLAVLRVQTNRGNDWQGPMFWTGSCLPQQGVA
                     VEDATKGSRVRVDCLRFKRWVDGPQWLEKNQPDFAQWLLGRKLAVSAPYSYMSYRYAT
                     EGGAMVAVDVLADQRLLRPRTRNNEEFLVAGRPALQWGHELAQAVRVSSGMMDGYLAI
                     PPFPYAAAAP"
     gene            9604..11211
                     /locus_tag="PK518_04650"
     CDS             9604..11211
                     /locus_tag="PK518_04650"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRM48233.1"
                     /translation="MQTEPTPQAQPQRIGVPRETFPGEKRVATVPDVVEKLIKLGFTV
                     AVESGAGDAANFSDDAYRAAGAEVVAGAAQLWSTSDIVLKVRPPSEDEVGLMRGGGTL
                     IDFIWPAQNPQLMEQLAARKATVLAIDCLPRTLSRAQKMDALTSTAGVSGYRAVIEAA
                     NAFGRYFNGQITAAGKVPPAKVFIAGAGVAGLAAIGTAANLGAIVRANDTRAEVADQV
                     KSLGGEFVKVDYEEEGSGGGGYAKVMSEGFQAAQRQMYATQAKDADIIITTALIPGKP
                     APRLITAEMVQSMKPGSVIVDMAAEQGGNCELTVPGEAVVRHGVTIIGYTDLASRLAK
                     QSSTLYATNLLRLMEELCKAKDGVAVVNMEDDAIRGLTVIKDGSVTWPAPPLKAPPAP
                     APKAAAAPMAEKKSAHGPGEPMSAKALSIVFAVGAVLFWLIGAYAPEAFLGHFTVFVL
                     ACFIGYMVVWNVTPALHTPLMSVTNAISSIIAIGALVQIAPPEAGVNGRPDGLIMWLA
                     FAALVLTAVNMFGGFAVTRRMLAMFRK"
     gene            11242..12654
                     /gene="pntB"
                     /locus_tag="PK518_04655"
     CDS             11242..12654
                     /gene="pntB"
                     /locus_tag="PK518_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HRM48234.1"
                     /translation="MSASLATVAYIGAAILFILSLGGLSNPETSRRGNLLGMVGMALA
                     VLATVLGPRVSSAGLAWIIAALVIGGGVGLYAAKVVKMTQMPELVALMHSLVGLAACL
                     VGFASYVDTSMPLQGVEKTIHEVEIYVGILIGAITFSGSLIAFGKLSGKIGGKPLLLP
                     ARHWLNLVGLLVVIWFGRAFVNAHDVQSGMAPLIIMTVIALLFGVHMVMAIGGADMPV
                     VVSMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCNAMNRNFISVIAGG
                     FGSGGGAPAKKGDAAEPQGEVVPVSSQETAEMLRESKNVVIVPGYGMAVAQAQHTVFE
                     ITQTLREMGVNVRFAIHPVAGRMPGHMNVLLAEAKVPYDIVMEMDEINEDFPDTDVAI
                     VIGANDIVNPSALEDPSSPIAGMPVLEVWKARHSIVMKRSMASGYAGVDNPLFYKENN
                     RMLFGDAKKMLDEVLAALRA"
     gene            12822..14501
                     /locus_tag="PK518_04660"
     CDS             12822..14501
                     /locus_tag="PK518_04660"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRM48235.1"
                     /translation="MTDRIWLKSYPPGVPADIDPTQYRSLVQLMEQAFRDYAGRTAYS
                     FMGKDVSFAETDAQSRQLGAYLQNLGLQRGDRVALMMPNVPQYPVAVAAVLRAGFVVV
                     NVNPLYTPRELEHQLKDSGAKAIVIIENFAATLQACIANTAVKHVVLCAMGDRLGILK
                     GMLVNYVVRNVKKLVPPFQLPGAVRFNDALAKGAGAALQPVDIGPDDIALLQYTGGTT
                     GVSKGAVLLHRNVIANVLQSEAWNAPALGKIPAGEQTAAVCALPLYHIFAFTVNMMLY
                     MRMGGKNILIPNPRDLAGTLKELSKHSFHSFPAVNTLFNGLANHPDFNTVDWSHLKLS
                     VGGGMAVQAAVAKLWLEKTGCPICEGYGLSETSPVACANPVTATEFSGTIGVPMPSTY
                     MRIIDDEGRDISATGQPGEIAILGPQVMAGYWQRPDETAKVMTEDGYFKTGDIGVMDE
                     RGYFKVVDRKKDMVLVSGFNVYPNEVEDVVALIPGVLECAVVGVPDEKTGEAVKLVIV
                     KKDPALTEAQVRDYCRTNLTGYKQPKVIEFRTELPKTPVGKILRRELRDKK"
     gene            complement(14560..15006)
                     /locus_tag="PK518_04665"
     CDS             complement(14560..15006)
                     /locus_tag="PK518_04665"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HRM48236.1"
                     /translation="MKTLVKLSLATLLSVAACWGTAQTLSSIRGSDVSTVDQAPEIKN
                     YQGSKPGGQALIQRTFAQQPPLIPHKVDGFDDITAEENTCLDCHIHKEVRGQKIPQIK
                     ESHLIKPVNADAPQVHNSRWQCNSCHVPQIDAKPLVENVFKGNAKK"
     gene            complement(15041..15940)
                     /gene="napH"
                     /locus_tag="PK518_04670"
     CDS             complement(15041..15940)
                     /gene="napH"
                     /locus_tag="PK518_04670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HRM48237.1"
                     /translation="MKPARNAFARDAIAAKGWWSAHRWLLLRRSVQLGVLALFLAGPW
                     LGWWVVKGNLNYSLTLDTLPLADPLVMLQSLLTGHVPERNAIIGVAIVLAFYLLVGGR
                     VYCAWVCPVNIVTDAASALRRRLDIRGGGDLPRQTRYWMLALILALAAGTGSIVWELV
                     SPVSLLPRGLIFGMGGGWLLIAGIFLFDLFVMKRGWCGHLCPVGAFYGLLGRVGLVRV
                     SATRREDCNDCLDCFAACPEQHVIRLPLKGAHKGVGPMIKDSDCTSCGRCIDVCAKDV
                     FQFVPRFAPNADDQARPGAQPEQ"
     gene            complement(15937..16776)
                     /gene="napG"
                     /locus_tag="PK518_04675"
     CDS             complement(15937..16776)
                     /gene="napG"
                     /locus_tag="PK518_04675"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HRM48238.1"
                     /translation="MTAPVSAAARREFMLDTAKMACGMGVIGLGLGLLNRQARALPAG
                     AIRPPGAGSETDFQSACIRCGLCVRDCPYDILQLATPAQQVATGTPFFVARSKPCEMC
                     EDIPCVKACPTGALDHGLTDINQAKMGLAVLVDHETCLNFLGLRCDVCYRVCPVIDKA
                     ITLEVQHNERTAKHTMFLPTVHSEHCTGCGKCEASCVLEVAAIKVYPIAQAKGELGAH
                     YRVGWEEKRKAGQSLVEERGLIDLPDRMPEGVTLPGHFDPGSSGGTGALGTGGQLPDG
                     APR"
     gene            complement(16844..19369)
                     /gene="napA"
                     /locus_tag="PK518_04680"
     CDS             complement(16844..19369)
                     /gene="napA"
                     /locus_tag="PK518_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HRM48239.1"
                     /translation="MQITRRELIKAHAVTAAATAIGVSVPGAQALAQSAGAADDIRWD
                     KGACRFCGTGCGVLIGVKDGRVVATQGDPDAPVNKGLNCIKGYFLSKIMYGKDRLTQP
                     MLRMKDGKFEKNGEFTRISWNQAFDIMEDKCKAALKAGGPRNIGMFGSGQWTIWEGYA
                     ASKLFKAGFRSNNIDPNARHCMASAVAGFMRTFGIDEPMGCYDDCEHADAFVLWGANM
                     AEMHPILWSRVTDRRLTAKHVKLHVLSTFTHRSCELADSELIFKPQSDLAILNYICNH
                     IIQSGAVNEDFVKKHVNFRKGVTDIGYGLRPNHPLEQVAGNNGYPGADGKPKGSPDAS
                     APISFDEFKAFVAEYTLDKAHELSGVPKDKLEALAKVYADPKTKVTSFWTMGFNQHTR
                     GTWVNNMIYNVHLLVGKISEPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPK
                     HREISEKEWKLPAGTIPDWVGLHAVAQSRALKDGKLNFYWTTTTNNMQAGPNINGEIW
                     PGFRNPANFIVVSDCYPTVSALAADLILPSAMWVEKEGAFGNAERRTQFWRQQVKAPG
                     QAKSDLWQYVEFSKRFKTDEVWPAELLEKAPEYKGKTLYDVLYANGQVNRFPLSDLQK
                     GFDNDESKELGYYLQKGLFEEYAGFGRGHAHDLADFDVYHKARGLRWPVVDNKETLWR
                     FREGYDPYVKKGEVLKFYGKPDGKAWIFALPYQPAAEMPDAEYDLWLSSGRVLEHWHT
                     GSMTRRVPELYRAMPDAWLYMHPEDAKKRGLQRGDTVKVMTRRGEVATKVETRGRNKP
                     PQGLVFMPFFDEHRLVNKLTLDATCPISKETDFKKCACKVVKA"
     gene            complement(19360..19719)
                     /locus_tag="PK518_04685"
     CDS             complement(19360..19719)
                     /locus_tag="PK518_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HRM48240.1"
                     /translation="MHDPFYKWGRILVIRLTPGYWQYPKPYLIEGSILNISSAIVYAR
                     PGTDATVRAQLPHIPGVEVHAAAEDGRLVVTIEAENDRGAMDTYEAIARMDDVLNIAM
                     IFQQTETHPDQELAKCK"
     gene            19966..20739
                     /locus_tag="PK518_04690"
     CDS             19966..20739
                     /locus_tag="PK518_04690"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRM48241.1"
                     /translation="MTTAILSALPEEQGGLVQALQAPECITHAGRSFWRGRLLGQPVL
                     LALSGIGKVAAATTATLLAERFGVARIVFTGVAGGLGDGVQVGDVVVAQDFLQHDMDA
                     SPIFPRWEVPGYGLARMPCDAALTTMLFEAASAYLTSDRAGFDPNNPAIAHRHAACAH
                     QGLIVSGDRFVSTSAESAALRQALGAAGHQVLAVEMEGAAVAQVCRDYGLPFAAVRTI
                     SDRADDSAHVDFPHFVRHVASRYADHIIQGLLRAMPGGR"
     gene            complement(20800..21786)
                     /gene="corA"
                     /locus_tag="PK518_04695"
     CDS             complement(20800..21786)
                     /gene="corA"
                     /locus_tag="PK518_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRM48242.1"
                     /translation="MLNIFSLVNGRLVQEEIDSLEDQAPQHPIWVDMESPTVLEKRWV
                     KQHYGLAIPLDVMDEDIEESARFYEEDNGELHIRSDFLVDDDEEPRSVRVAFILNQHN
                     TELKSCGVLFSIREEDIPVFRLLRMRARRAPGLIEDAKEVLLSLFDTDVEYSADTLEG
                     IYDDLKKVSTQVLEGHVTDDHAQKVLADIAWQEDLNGRIRRNMLDTRRAVSFMMRSKM
                     LNAEQFEDARQILRDIESLDSHTQFLFDKINFLMDATVGFLNINQNKIIKIFSVASVA
                     LLPPTLIASVYGMNFRLMPELEWEHGYIYALCLMIASAVVPMLYFRKRGWLK"
     gene            complement(21988..22749)
                     /locus_tag="PK518_04700"
     CDS             complement(21988..22749)
                     /locus_tag="PK518_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRM48243.1"
                     /translation="MSLELDARQRAMLLEMGVTVWAPAPAAAPAAPPTNEAAPVTRPA
                     PAATPARPAAQAAAAPPPTTRRQAPTQAAAPTPAAAMAQAGGLLLRPPIALYPQADPT
                     ATPPELGSGWLVLLESPTPAEPLAGDSGQLLDNMLRAMRLHRHPRCFVATLSQPQPGR
                     PAEGEPPAAGLQQALTTLRPAMVLVLGLGAARVVLGSREPLGRLRASAHQLADGTPAV
                     VSYDPAYLLRAPEAKAAAWIDLCRALALVRRQPGA"
     gene            complement(22746..23222)
                     /gene="rimI"
                     /locus_tag="PK518_04705"
     CDS             complement(22746..23222)
                     /gene="rimI"
                     /locus_tag="PK518_04705"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRM48244.1"
                     /translation="MNANPRPADAMQASFAPLTTERLDALLAVEQAAYSHPWTRGNFI
                     DALAAGYQAQLLVAGGQILGYFVAMPGVQEAHLLNITVAPAFQRQGWAHVMLDALALW
                     ARGQGAQWLWLEVRASNTRARQVYEAHGYQQVGLRKRYYPSSDGEREDAVVMSLPL"
     gene            complement(23219..>23355)
                     /locus_tag="PK518_04710"
     CDS             complement(23219..>23355)
                     /locus_tag="PK518_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRM48245.1"
                     /translation="APALLAAGRAVPADAALPRYIRDKVAQTTAEREAARRASAAQAS
                     "
BASE COUNT         3919 a         7603 c         7887 g         3946 t
ORIGIN      
        1 tctgggagag ggtgggggtg agggctcgcg gaccagtctg aggcatgtgc ctagtcaggt
       61 aaaacagggc tgcagcgctt gtgcatcaag cgcaaggagc tattaaatca atagtattgg
      121 gtcgcgatcc atgaatacca agcaacaact caaggccctg gccggggcgc gccgcggcgc
      181 cctggtgccc ggcgcgttca atgccctgtc ggccaagctc atcgaagacc tgggcttcga
      241 ggccatctac atcaccggcg ccggcgtgac caatatgtgg tttgccatgc ccgaccaggg
      301 cttcatgggc ctgcatgaaa tcgccgagca cacggcgcgc atccgcgatg cggtgcagct
      361 gccgctgatc gtcgatgccg acaccggctt tggcaatgcg ctcaacgtct atcacacggt
      421 gcgcacgctg gagcgggccg gggccgactg catacagctg gaagaccagg tgcatccgaa
      481 gcgctgcggt catttctcgg gcaaggaggt catcagcacc gaggaggcgg ttgccaagat
      541 caaggccgcc gtcgatgccc ggcgcgagag cgccactctc atcatggcgc gcaccgacgc
      601 cgccgccacg cacggcttcg aggccgccgt ggagcgcgcg cagcgctttg ccgaggccgg
      661 cgccgacatc ctgttcgtcg aggccgtgac ccaggccgag gaggtgcgcg ccctgccgca
      721 acgcctggcc acgccccagc tgatgaacat ggtgataggc ggcaagacgc ccatcttcga
      781 cgcggagcag ctgggcgagc tgggctacgg cctggtgctc tacgccaacg ccgcgctgca
      841 gggcgcggtg atgggcatgc acaaggccct gagcacgctg cgcgacgaca agcaggtgct
      901 ggagagcagc ggcctggtca ccccctttgc cgagcgccag cgcctggtgg gcaagccggg
      961 ctgggatgcg ctggaggaaa aataccgggg gtagccgagc gcccattcat cacgcaacga
     1021 aatcgcgggg cagcaggctg cccttcagat acaacggatg gcggggtgta ccgttcttgg
     1081 tcagttgcag gtactggagg ggcgcggccc ttcgcaatat ctgcaccacc gctgcggcac
     1141 gcgcgcgcag cggctggggt ggcaagccat aggccaaaac aaccctctcg gcctggcgcg
     1201 ccatgtgtag cagccttgca tcgttttcag ggccgaccgg gtcgtccgcc tccacgagcc
     1261 gacggctgtc cgtcacacgg taggcgtgga cgttaccaac cagctgcccg ccatagcccc
     1321 aagcccgcgc atatttgccc gttttgatga gcgttgggtc ggcgtgctcc acccccgcta
     1381 ccgaagggtt catcagcaag aacatcacca acgcccggtt tgtgtcccac acctccgaca
     1441 ggctgtagcg gtaccgtccg caaccggaaa actgcgcctg aaccacagaa cccgagggcc
     1501 attggggttt gtgttttccg ccaggatcgt ggtgcatggt gtgtctgtct gctcttggcc
     1561 cgcgcattca ctcgatgcgg ccaatgatag ggaggatggg ccgtgctaca gcccatcgat
     1621 cgtgcgcctt ggaatttttc cgatttggcg caaaatgcgc caaatggagt ctgttatggc
     1681 acataccgct tttgcatccg tcaagctccc cagcccgctg gtggaacagg cccgccaggc
     1741 tgcgcagccc atgcgccgct ctgtcgccag tcagatcgaa tactgggcca ccctgggcca
     1801 gatcgtcgag cacaccggcc tgagcgtgca ggaagcacgc gccgccatcg agcaatacga
     1861 ggcccggcac acgcctacac cacctgcatc gatcgatgcg ctcaccaccc gcctggtggc
     1921 ggcgcaaacg cagggttcgc tggctgaacg ggtgcgccag gtagtgcaag aaaatcaggc
     1981 acgcgccagc tgaaccccat gaccaccgcc acgcccgggc cgttgtggct gcacttgctg
     2041 ggcgggcaaa tccaccctgt accgcgccct ggtgcgcgag ggcatcctcg ggccgccgct
     2101 ggagttcgtg aacgccgatc cgcacgagcg cgcgcacctg cagcacatca ccagcggcga
     2161 agaacgctcg cgggcggcaa ggacctgggc cgatggccgg cgcgccacat tgctggccac
     2221 acgcacgccc tttgcgaccg aaacggtgtt ctcgcacccg tccaagcttg atctgattgc
     2281 caacgcgcag cgccagggct ttaccgtggc actgtacgtg gtcgcgctgg acgatccgca
     2341 gcgcctgttg gggcgcgtgg cgcagcgcgt gcgcgaaggc ggccacccgg tgccgcccga
     2401 acgcatcctg gcgcgctacc cgcgcaccat ggccctgctg gcgcaggcgg tgcggcaggc
     2461 ggacgtggct tatctgtacg acgcggctga agtgcgggcc ggcggcccgc agctggtggc
     2521 cgtgcgcagt gcgggcaaca gcacgccggt ggttgcccct ctgcctgcct gggcgcaggc
     2581 catgctgggt gcccagcgcc aatgattggc gcggccggtg ctatgtctgg gttgcttcta
     2641 aactaatagc tttttgcgca gacatggagc gcgccaaggc aataaacgcc tgcaaatagt
     2701 cggtctgcag atcggccacg cgcgcgccca ggtggatctg cttggcaata ccgcgcggcc
     2761 ccaggcgcac cgggaccacg tccatgcggg cggcgtattc ctgcaccagc cagcgcggca
     2821 gggcggccac gccgcggccg ccggcgacca tctgcagcat gatgtccgtc gtctcgatcg
     2881 tcttgtggcg cgccggtgcc acgccggcgg gcagcaggaa gcgggtgtag atgtccagcc
     2941 gctccggcgc cacggggtag gcgatcagca cctcctgcgc cagctgccgc ggcagcgcgt
     3001 gggccgcgcc ggccagcgca tggccgcgcg ccaccaccag cacctgctcg tagtcgaaca
     3061 ccggctcgaa gtgcaggccg gggcgctgca ccgggtcggg cgtgaccagc aggtcgatct
     3121 catggtcctg cagcgcgtcc aggccgccga actgaaagcg ctgtttcacg tccacgtcca
     3181 cgtcgggcca ggccgccaga tagggcgaca ccaccttcag cagccactgg tagcagggat
     3241 ggcactccat gcctatgcgc aggctgccgc gctcgccttg cgcgaactgg cgcagccgtt
     3301 cctcggccag ctgcagctgc ggcagcacgc ggccggccag ctccagcagg tagcggccgg
     3361 cctgcgtcag gcgcaggctg cggccctcgc gcagccagat gggggtgccc agctgctgct
     3421 ccagcttctt catgctgtgg ctcagggccg actgggtgac gcacagcctg tctgcggcgg
     3481 cggtgagcga gccttggcgc tcgacctcct ggatcacggc gagatggatg cggtccagca
     3541 tttcaaatga acaaaattca tgaatcagtg agataaaacc attttacgtc atgggccgcg
     3601 ctgcctacga tgcgcccatt catcattcaa agggagcggc acatgaccac catccacaac
     3661 ctcggctttc cgcgcatggg cgcgcggcgg gagctgaaat ttgcactcga gtcctactgg
     3721 cgcggcgaat cctcgcgcgc ggcgctgcag gagcagggcg cgcggctgcg ccagcggcat
     3781 tggcaggagc aggcggggct ggactgggtg ccggtgggcg atttttcgtt ctacgaccag
     3841 gtgctggaca tgagctttac cctgggccac ctgcccgagc gcgtgcaggg ctttcatggc
     3901 gacgcgctgg acaactactt ccgcgtggcg cgcgggcgca gcgcccaggc cgaggctcac
     3961 cagggttgct gcggcgcagt ggccgcgggc gagatgacca agtggttcga caccaactac
     4021 cactacatcg tgcccgaatt caccgccgcc acgcagttcc ggctggacgc ctcgcgcctg
     4081 ctggcccagt tggccgaagc cggcgcccag ggcgtgccgg caaagccggt gctcatcggc
     4141 ccggtgacct atctggcgct gggcaaggcc aaggacggct cgaaccggct ggccctgctg
     4201 gagcgcctgc tgccggtcta tgccgagttg ctcgataccc tggctgcgca tggcgtgcag
     4261 tgggtgcaga tcgacgaacc catcctggtc accgagctgg acgccgactg gcggcacgcc
     4321 ttcaccaccg cctaccacca gctcaagtcc tgccgcgtaa agctgctgct ggcgacctat
     4381 ttcggccagc tgcaggacaa cgcctacctg gccgccaacc tgcccgtggc cggcctgcat
     4441 gtggacgccg tgagtgaccc ggacggcgtg ctgcccctct tgggcctgct gccggcgcac
     4501 aaggtgctgt cgctgggcgt ggtcaatggc cgcaatgtct ggaaaaccga tttgaacgcc
     4561 acgctcgatt ggctggagcc gctggccgcg cgcctgggcg agcgcctgtg gctggcgcca
     4621 tcctgctcgc tgctgcatgt gccggtcgat ctggcgcagg agcagcggtt ggacgccgag
     4681 ctcaagtcct ggctggcctt tgcgctgcaa aagctggacg agctgcggct gctgggccgg
     4741 gcgctgcgcg acgggcgtgg cgcggtcgag gccgagctgg ccgagaacca cgccgcgctg
     4801 gtcgcacgcc gcacctcgcc gcgcgtgcac agcccggcgg tgcgggcggc ggtggcggcc
     4861 atcacggccg acatgggccg gcgccaaagc ccctacgcca gccgcgcggc caggcaggcg
     4921 gcgctgctgc agctgccggc gtacccgacg acgacgattg gctcgttccc gcagaccgcg
     4981 caaatccgcc aggcgcgcag cgccttcaag gccggccaga tcgatgccgc ggcctaccgt
     5041 gaggcgatgc gtgccgagat cgcgcgcagc gtgcgcgcgc aggaagagct gggcctggat
     5101 gtgctggtgc atggcgaggc cgagcgcaac gacatggtcg aatactttgg cgagcaactc
     5161 gacggctacg ccttcagcca gttcggctgg gtgcagtcct acggctcgcg ctgcgtcaaa
     5221 ccgcccatcc tgtttggcga tatccagcgc ccgcgcgcca tgaccgtgga ctggacgcgc
     5281 tttgcccagt cgctgaccga caggcccatg aagggcatgc tcaccggccc ggtgaccctg
     5341 ctcaactggt ccttcgtgcg cgacgaccag ccgcgcgcgg ccacctgccg gcagctggcg
     5401 ctggccatgc gcgccgaggt gctggatctg gagcgcgccg gggtgcgcgt gatccagatc
     5461 gacgaggccg cgctgcgcga gggcctgccg ctgcgccgcg cgcagtggtc gcaatacctg
     5521 gactgggcgg tgcaggcctt ccgcatcacc gccaacggtg tgcgcgatga aacccagata
     5581 cacacgcata tgtgttattc cgaattcaac gacatcatgg ccgcgatcgc cgccatggac
     5641 gccgacgtga tcaccatcga gaccagccgt tccgacatgg agctgctcga cgcgtttgag
     5701 gatttccagt atccgaacca gatcgggccg ggcgtttacg acattcattc acccaatatt
     5761 cccgcggcac agcagatgat tgcgctcata aaaaaagcag ccgatcggat tcctgcggag
     5821 cgcctgtggg tcaacccgga ctgcgggctc aagacccgcc aatgggccga ggtggtgcca
     5881 gccctgaccg agatggtggc tgctgccagg gcgttgcgcg ccggcagcag ttgagcacgg
     5941 cgtgccggca aggggcttgc atgtgaggtg ttctgtgcaa aaatcgaaac tcgattacac
     6001 gaacagaaag agaccgagcc atgcaagtcc gccgcgccac caagatcgtc gccaccctgg
     6061 gccccgcctc cagcgacccg cagctgctgg agcagatgat tgccgcgggc gtgaacgtgg
     6121 tgcgcctgaa cttcagccat ggcaaggcca gcgaccatgt ggatcgcgcc accatggtgc
     6181 gcgaggctgc ccggcgcgcc gggcgcgagg tggccatcat ggccgacctg cagggcccga
     6241 agatccgcgt cggcaagttt gccgatggca aggtgatgct ggagccgggc gcgcccttcg
     6301 tgctcgacgc ctcgcgcacc gagcccggcg acctgcaggg cgtgggcctg gactacaagg
     6361 agctgccgcg cgacgtcaag cccggcgaca cgctgctgct caacgacggc ctgatcgtgc
     6421 tcactgtgga tgccgtgcgc ggcgatgcgg tgcataccac cgtcaagctg ggcggcgagc
     6481 tgtccaacaa caagggcatc aacaagcagg gcggcggtct gacggcgccg gcgctgacgg
     6541 ccaaggacat ggaggacatc aagacggcga tgagcttcca ggccgactat gtggcggtga
     6601 gctttccgaa gaacgccacc gacatggaga tggcgcgcca gctgtgcaac gtggccgcgg
     6661 ccgagttccg ccacaggccg ggcctgatcg caaaaataga gcgcgccgag gccattccgc
     6721 gcctggagga aatcctcaag gtgagcgacg gcatcatggt cgcgcgcggt gacctggcgg
     6781 tggaggtggg caacgccgcc gttccggcgc tgcagaagaa gatgatccgc atggcgcgcg
     6841 acatggacaa ggtggtcatt accgctacgc agatgatgga gagcatgatc accaaccccg
     6901 tgcccacgcg cgccgaggtc agtgacgtgg cgaatgccgt gctcgacggc accgacgccg
     6961 tgatgctcag cgccgagacc gccgctggca agtacccgct ggagacggtg gaggaaatgg
     7021 ccaagatctg cgccgccgcc gaggccgccg aggacccgga tcgcgacgcc gacttcgacg
     7081 gccagaccct ggggcgcatc gaccagtccg tggccatggg cgcgctgttc acggcgcacc
     7141 acctgggcgc caaggccatc gtggccatga ccgacagcgg ctccaccgcg ctgtggatga
     7201 gccgtaaccg catccatatc ccgatctatg cgctcacgcc caaggtggcc acgcagcggc
     7261 gcatggccat gtaccgcaac gtgcgcccgc tgctcatgga caccagcgcc gaccgcgaca
     7321 cggcgctgga gcaggccgaa aaccacctca agatgcgcaa catcgtgctg cagggcgacc
     7381 tgtatgtgat cacctgcggc gagcccatgg gccagccggg tggcaccaat atgctgaaga
     7441 tctgccgggt gcattgatcc tgcgcgcctt ggcgcgcatg tgggcatggg tggcccgggt
     7501 ggggcaggcg gttaaaatcg gcggttacca agcggttacc accgcttttc ctcaaccctc
     7561 gaacggatcc tccccatgcc tctcgtctcg atgcgcgaac ttctcgacca tgccgccgac
     7621 aacggctatg gcatccccgc cttcaacgtc aacaacctgg agcaggtgca ggccgtgatg
     7681 acggccgccg acgaggtggg cgctcccgtg atcctgcagg ccagcgccgg cgcgcgcaaa
     7741 tatgccggcg agagcttcat caagtacctg atcgccgccg ctgccgaggc ctatccgcat
     7801 attccgctgg tcatgcacca ggatcacggc acctcgcccg ccatttgcca gggtgcgctg
     7861 gatctgggct ttggctcggt gatgatggac ggcagcctca aggaagacgg caagaccccg
     7921 gccgacttcg actacaacgt gcgcgtcacg cggcaggtgg tcgaaatggc ccacaaggtc
     7981 ggcgccacgg tggagggcga actcggctgc ctgggcagcc tggagaccgg cgaggccggc
     8041 gaggaagatg gcatcggcgc ggtgggcaag ctcagccacg cccagatgct gaccgacccc
     8101 gaggaggccg cccagttcgt caaggccacg cagctcgacg cgctggccat agccatcggc
     8161 accagccacg gcgcctacaa gttcacgcgc ccgcccacgg gcgacgtgct ggcgatctcc
     8221 cgcgtcaagg aaatccacgc acgcattccc aacacccatc tggtgatgca cggctcgtcc
     8281 tccgtgccgc aggatctgct ggacatcatc aaccagtacg gcggcaagat gaagcagacc
     8341 tacggcgtgc cggtcaagga aatccaggaa gccatcaagc acggcgtgcg caagatcaac
     8401 atcgacaccg acatccgcat ggccatgacc ggcgcggtgc gcaagttcca gttcgagaac
     8461 ccggacaagt tcgacatgcg cgagtggatg aagcctgcac gcgaggccgc caaggcgatc
     8521 tgcaagcagc gctatctgga gttcggctgc gagggccagg ctgccaagat caagggctac
     8581 acgctcgacg aaatggcggc caggtacgct gcaggcgcgc tgcgccaggt ggtgaactga
     8641 tgccacgctg agcgcacgcc atgaaaagac ccggccccgc aggggagccg ggtttttttc
     8701 gcgctatgca ccgctgcgcc atttgtcccc caggtgtgcg ggcgctaaag ttctttcgtg
     8761 taagcttggg gtcagtaggc ccgagcggtc atgctgtggg cggttcttgc tttttccctt
     8821 catgaatctg gcgatttcga caatcaaatg cctgttgcca tgcgccctgc tgacgctgac
     8881 agcatgcgcg cccctggctt cgcagcaggc ggaatacccc gccggccgtg cgcgcctggc
     8941 gttgccggct ggagcgtggg aagatctggg cacctcggac caggcggttc gcctgttgcc
     9001 gcagcccgat ggccgcattg cgctgcaaag ccgcgtgctg ggcctgcgtg gcgcacagca
     9061 ggagctgctg gccgtgctgc gcgtgcagac caaccgaggc aacgactggc aaggcccgat
     9121 gttctggacg ggcagctgtc tgccccagca gggcgtggcg gtagaggacg ccaccaaagg
     9181 cagccgcgtg cgcgtggact gcctgcgctt caagcgctgg gtggatggcc cgcagtggct
     9241 ggaaaagaac cagccggatt tcgcccagtg gctgcttggc cgcaagctgg ccgtgagcgc
     9301 accctattcg tacatgagct accgctatgc caccgagggt ggtgccatgg tggcggtgga
     9361 tgtgctcgcc gatcagcggc tgctgcgccc caggacgcgc aacaacgagg aattcctggt
     9421 cgcgggccgg cccgcgctgc aatggggcca tgagctggcc caggcggtgc gcgtgagcag
     9481 cggcatgatg gacggctacc tggccattcc gcccttcccc tacgcagcgg ccgcgccctg
     9541 acgcacgcaa gtccccagcc tgccggactt gcacaacgat taccaaactc aaggagacct
     9601 cccatgcaga ctgaacccac gccccaggcg cagccacaac gcattggcgt gcccagggaa
     9661 accttccctg gcgagaaacg cgtcgccacc gtgcccgatg tggtggaaaa actgatcaaa
     9721 ctcggtttca cggtggccgt ggaatccggt gcgggagatg cggccaattt cagcgatgac
     9781 gcctaccgcg ccgccggtgc cgaagtcgtc gctggggccg cgcagctgtg gtccacgtcc
     9841 gacatcgtgc tcaaggtgcg cccgcccagc gaggacgaag tggggctgat gcggggcggc
     9901 ggtacgctga tcgacttcat ctggccggcg cagaaccccc agctgatgga gcagctggcg
     9961 gcgcgcaagg ccacggtgct ggccatcgac tgcctgccgc gcacgctctc gcgcgcgcag
    10021 aagatggatg cgctcacctc caccgcgggt gtgtccggct accgcgccgt gatcgaggcg
    10081 gcaaatgcct ttggccgcta tttcaatggc cagatcacgg ccgcgggcaa ggtgccgccg
    10141 gccaaggtgt tcattgccgg cgccggcgtg gcgggcctgg cggccatcgg cacggcggcc
    10201 aatctgggcg ccatcgtgcg cgccaacgac acgcgcgccg aggtggccga ccaggtcaag
    10261 tcgctgggcg gcgagttcgt caaggtcgat tacgaagaag agggctcggg cggcggcggc
    10321 tacgccaagg tcatgagcga gggcttccag gccgcgcagc gccagatgta cgccacccag
    10381 gccaaggacg cagacatcat catcaccacc gcgctgatcc ccggcaagcc ggcgcccagg
    10441 ctgatcaccg ccgagatggt gcagagcatg aaacccggca gcgtgatcgt ggacatggcc
    10501 gccgagcagg gcggcaactg cgagctgacg gtgcccgggg aggccgtggt gcgccatggc
    10561 gtgaccatca tcggctacac cgatctggcc tcgcgcctgg ccaagcagtc gtccacgctg
    10621 tatgccacca acctgctgcg cctgatggag gagctgtgca aggccaagga cggagtggcc
    10681 gtggtgaaca tggaggacga cgcgatccga ggcctcacgg tcatcaagga tggcagcgtg
    10741 acctggcccg ccccaccact gaaggcgccg ccggcccccg cgccgaaggc ggctgctgcg
    10801 cccatggccg agaaaaagtc tgcccatggc ccgggcgagc cgatgtcggc caaggcgctg
    10861 agcatcgtct tcgccgtcgg cgcggtgctg ttctggctca taggcgccta cgcgcccgag
    10921 gcgttcctgg gtcacttcac ggtgttcgtg ctggcctgct tcatcggtta catggtggtg
    10981 tggaacgtca cgccggcgct gcacacgccg ctgatgagcg tcaccaacgc catctccagc
    11041 atcatcgcca tcggcgcgct ggtgcagatc gcgccgccgg aggccggcgt caatgggcgg
    11101 cccgacgggc tcatcatgtg gctggccttt gccgccctgg tgctcacggc cgtcaacatg
    11161 ttcggcggct ttgcggtgac gcgccgcatg ctggccatgt tccgcaaata acaacgacaa
    11221 gaacacagag gagcacagaa catgtccgca agtctcgcta ccgtcgccta tatcggcgcc
    11281 gccatacttt tcattctcag cctgggaggg ctgtccaacc ccgaaacctc gcgccgcggc
    11341 aacctgctgg gcatggtcgg catggcgctg gcagtgctgg ccacggtgct cgggccgcgc
    11401 gtcagcagcg cggggctggc ctggatcatt gccgcgctgg tgatcggcgg cggtgtcggc
    11461 ctgtatgccg ccaaggtcgt gaagatgacc cagatgcccg agctggtcgc gctgatgcac
    11521 agcctggtgg gcctggcggc ctgcctggtg ggtttcgcca gctatgtcga tacctcgatg
    11581 ccgctccagg gcgtggaaaa aaccatccac gaggtggaga tctacgtcgg catcctgatt
    11641 ggcgccatca ccttctcggg ctcgctcatc gcctttggca agctcagcgg caagatcggc
    11701 ggcaagccgc tcttgctgcc cgcgcgccac tggctcaacc tggtgggcct gctggtggtg
    11761 atctggttcg gccgcgcctt cgtgaacgcg catgacgtgc agtccggcat ggcgccgctg
    11821 atcatcatga ccgtcatcgc cctgctgttc ggcgtgcaca tggtcatggc cattggcggc
    11881 gccgacatgc cggtggtggt ctccatgctc aacagctact ccggctgggc ggcggcggcc
    11941 acgggcttca tgctctccaa cgacctgctc atcgtcaccg gcgctctggt gggctcctcg
    12001 ggcgccatcc tctcctacat catgtgcaac gcgatgaacc gcaacttcat cagcgtcatc
    12061 gccggcggtt tcggctccgg cggcggtgca ccggccaaga agggcgacgc cgccgagccc
    12121 cagggcgagg tggtgccggt gagcagccag gagacggccg agatgctgcg cgagtccaag
    12181 aacgtggtca tcgtgcccgg ctacggcatg gccgtggccc aggcccagca cacggtgttc
    12241 gagatcaccc agaccctgcg cgagatgggc gtcaatgtgc gctttgccat ccaccccgtg
    12301 gccggccgca tgccgggcca catgaacgtg ctgctggccg aggccaaggt gccctacgac
    12361 atcgtgatgg agatggacga gatcaacgag gacttccccg acaccgacgt ggccatcgtc
    12421 attggcgcca acgacatcgt caaccccagc gcgctggaag acccgagcag cccgattgcc
    12481 ggcatgccgg tgctggaggt ctggaaggcg cgccattcca tcgtcatgaa gcgctccatg
    12541 gcctcgggct atgcgggtgt ggacaaccca ctgttctaca aagagaacaa ccgcatgctg
    12601 tttggcgacg ccaagaaaat gctcgacgag gtgctggcgg cactcagggc ctgacgatcg
    12661 ccactttaaa aaagaacggc cgtgctaaaa ttcgacggct ttctcgtcgc cggactgcgc
    12721 cagggtcgat aagcccgaga tcaggggctg cggccgtacg aaatgagcag gcttcggggg
    12781 gatttccatg cccctcatga caagaaccag gaggcttgga gatgaccgat cgcatttggc
    12841 tcaagagtta cccacccggc gtgcccgccg acatcgaccc gacgcaatac cgctcgctgg
    12901 tgcagttgat ggagcaggcg tttcgcgact atgcgggccg caccgcctac agcttcatgg
    12961 gcaaggacgt cagttttgcc gaaaccgatg cacagagccg tcagctcggt gcctatctgc
    13021 aaaacctggg cctgcagcgc ggtgaccgcg tggcgctgat gatgccgaat gtgccgcagt
    13081 atccggtggc cgtggcggcc gtactgcgcg cgggctttgt ggtggtgaac gtgaacccgc
    13141 tgtatacgcc gcgagagctc gagcaccagc tcaaagactc gggcgccaag gccatcgtca
    13201 tcatcgagaa cttcgccgcc acgctgcagg cctgcattgc caatacggcc gtcaagcatg
    13261 tggtgctgtg cgccatgggc gaccggctgg gtatcctcaa gggcatgctc gtcaactatg
    13321 tggtgcgcaa tgtcaagaag ctggtgccgc cgtttcagct gccgggcgcc gtgcgtttca
    13381 atgacgcgct ggccaagggg gcgggtgccg ctcttcagcc ggtggacata gggcctgacg
    13441 acattgccct gctgcagtac acgggcggta ccacgggcgt gagcaagggg gccgtactgc
    13501 tgcaccgtaa cgtgatcgcc aacgtgctgc agtccgaggc ctggaatgcg ccggccctgg
    13561 gcaagatacc ggcgggcgag caaaccgcgg cggtgtgcgc gctgccgctc taccacatct
    13621 tcgccttcac ggtgaacatg atgctgtaca tgcgcatggg gggcaagaac atcctgatcc
    13681 ccaacccgcg cgatctggcg gggacgctca aggaactgtc caaacacagc ttccacagct
    13741 ttccggccgt caacacgctg ttcaacggcc tggccaatca cccggatttc aacacggtgg
    13801 actggagcca tctgaagctc tcggtgggag gtggcatggc cgtgcaggcg gccgtggcca
    13861 agctgtggct ggagaagacc ggctgtccta tctgcgaggg ctatggcctg tccgagacca
    13921 gcccggtggc ctgcgccaac ccggtcacgg caacggagtt ctcgggcacg atcggtgtgc
    13981 ccatgccgag tacctacatg cgcatcatcg acgatgaggg gcgcgacatc tcggccaccg
    14041 gccagcctgg cgagatcgcc atcctcggcc cgcaggtcat ggccggctat tggcagcgcc
    14101 ccgacgagac cgccaaggtc atgaccgagg acggctactt caagaccggc gacatcggcg
    14161 tcatggacga gcgtggctac ttcaaggtgg tcgatcgcaa gaaggacatg gtgctggtca
    14221 gcggcttcaa cgtctacccc aacgaggtgg aggacgtggt ggccctgata cccggggtgc
    14281 tggaatgcgc cgttgtcggt gtgcccgacg agaagacggg cgaggccgtg aagctggtca
    14341 tcgtgaagaa agacccggcc ttgaccgagg cccaggtgcg cgactattgc cgcaccaacc
    14401 tgacgggcta caagcagccc aaggtgatcg agttccgcac cgagctgccc aagaccccgg
    14461 tgggcaagat attgcggcgc gagttgcgcg acaagaagtg atcctgcccc ggcagggccg
    14521 gggggaggcg actggcgggc gctgcagaag cggcccgcct tattttttgg cgttgccctt
    14581 gaagacgttt tccacgagtg gcttggcgtc tatctgtggc acatggcagg aattgcattg
    14641 ccagcgcgag ttgtgcacct gcggggcatc ggcgttgacc ggcttgatca ggtggctctc
    14701 cttgatctgc gggattttct gaccgcgcac ctccttgtgg atgtggcagt ccaggcaggt
    14761 gttttcctcg gcggtgatgt cgtcaaagcc gtccaccttg tgcggtatca gcggcggctg
    14821 ctgcgcgaag gtgcgctgga tcaacgcctg gccgcccggc ttgctgccct ggtagttctt
    14881 gatctcgggt gcctggtcca cggtcgagac atcgctgccc cggatgctgc tcagcgtctg
    14941 cgccgtgccc cagcacgccg ccacggacag cagggttgcc agagagagtt tgacgagcgt
    15001 cttcatgatg ggcttctcca cgggtgaaag ttgtttgagg tcattgttct ggttgggcgc
    15061 cgggccgggc ctggtcatcc gcgttgggcg caaagcgcgg tacgaactgg aaaacatcct
    15121 tggcgcacac atcgatgcag cgcccgcagc tggtgcagtc gctgtccttg atcatcggcc
    15181 ccacgccctt gtgtgcgccc ttgagcggca gccggatgac atgctgctcg gggcaggcag
    15241 caaagcagtc caggcaatcg ttgcaatcct cgcgccgcgt ggccgatacg cgcaccaggc
    15301 cgacgcgccc cagcaggccg tagaacgcgc ccaccgggca caggtggccg caccagccgc
    15361 gcttcatgac gaacaggtca aacaggaaga tgcccgcgat cagcagccag ccgccgccca
    15421 tgccgaagat caatccgcgc ggcagcagcg acaccgggct caccagctcc cagacgatgc
    15481 tgcccgtgcc cgccgccagc gccaggatca gcgccagcat ccagtagcgc gtctgacgcg
    15541 gcaggtcgcc accgccgcgt atgtccagcc tgcgccgcag cgcgctggcg gcgtcggtga
    15601 cgatgttcac cgggcagacc caggcgcagt acacgcgccc gcccaccagc aggtaaaagg
    15661 ccagcacgat agccacgccg atgatggcgt tgcgctccgg tacatggcca gtcagcagcg
    15721 actgcagcat caccagcggg tcggccagcg gcagtgtgtc cagcgtcagg ctgtagttga
    15781 ggttgccctt gacgacccac cagcccagcc agggcccggc caggaacagt gccagcacgc
    15841 ccagctgcac gctgcggcgc agcagcagcc agcggtgcgc gctccaccag cccttggcgg
    15901 cgatggcatc gcgggcgaag gcgttgcgtg cgggcttcat cgtggtgctc cgtcgggcag
    15961 ttggccgccc gtgcccaacg cgcccgttcc gccactgctg ccagggtcga aatggccggg
    16021 cagcgtgacg ccctcgggca tgcggtcggg caggtcgatc aggccgcgct cttcgaccag
    16081 cgactggccg gccttgcgct tttcttccca gcccacgcgg taatgcgcgc cgagttctcc
    16141 cttggcctgg gcgatggggt agaccttgat ggccgccacc tccagcacgc aggaggcctc
    16201 gcacttgccg cagccggtgc aatgctcgga atgcaccgtg ggcaggaaca tggtgtgctt
    16261 ggcggtgcgt tcgttgtgtt gcacctccag cgtgatggcc ttgtcgatca ccgggcagac
    16321 gcggtagcac acgtcgcagc gcaggcccag gaagttcagg caggtctcgt gatcgaccag
    16381 caccgccagg cccatttttg cctggttgat gtcggtcagg ccgtgatcca gcgcccccgt
    16441 ggggcaggcc ttgacgcagg gaatgtcctc gcacatctcg cagggcttgc tgcgcgccac
    16501 aaagaagggc gtgcccgtgg ccacctgctg cgccggcgtg gccagctgca ggatgtcgta
    16561 ggggcagtcg cgcacgcaca ggccgcagcg tatgcaggcg gactgaaagt ccgtctcgct
    16621 gcccgcgccc ggggggcgta tggcgcctgc cggcagggct ctggcctggc ggttgagcag
    16681 ccccaggccc aggccgatca cgcccatgcc gcaggccatc ttcgcggtgt cgagcatgaa
    16741 ttcacggcgc gcggcggcgc tgacaggagc ggtcatggcg gctaagtggg tgcagcgatg
    16801 ggagtgggcc gggcgcatgc aggttgcgcg cccgggctgt gggtcaggcc ttcacgacct
    16861 tgcaggcgca cttcttgaaa tcggtctcct tggagatcgg gcaggtggcg tccagcgtca
    16921 gcttgttcac caggcggtgc tcgtcaaaga agggcatgaa caccaggccc tgcggcggct
    16981 tgttacgccc gcgcgtctcg accttggtgg cgacctcgcc acggcgcgtc atcaccttga
    17041 cggtgtcgcc gcgctgcagg ccgcgcttct tggcgtcctc ggggtgcatg tagagccagg
    17101 catcgggcat ggcacggtac agctcgggca cgcggcgcgt catcgagccg gtgtgccaat
    17161 gctccagcac gcggccggaa gacagccaca ggtcgtactc ggcatccggc atctcggccg
    17221 cgggctggta gggcagcgca aagatccagg ccttgccgtc gggcttgccg tagaacttga
    17281 ggacctcgcc cttcttcaca taggggtcgt agccctcgcg gaagcgccac agcgtctcct
    17341 tgttgtccac caccggccag cgcaggccgc gtgccttgtg atagacgtca aagtcggcca
    17401 ggtcatgcgc atggccgcgg ccgaagccgg cgtattcctc gaacaggccc ttctgcaggt
    17461 agtagcccag ctccttggat tcgtcgttgt cgaaaccctt ttgcagatcc gacagcggga
    17521 acctgttcac ctggccgttg gcatacagca cgtcgtacag ggtcttgccc ttgtattcgg
    17581 gggccttctc cagcagctcg gcgggccaga cctcgtcggt cttgaagcgc ttggagaact
    17641 cgacgtactg ccacaggtcg gacttggcct ggccgggagc cttgacctgc tggcgccaga
    17701 actgcgtgcg ccgctcggcg ttgccaaagg ccccttcctt ctccacccac atggccgagg
    17761 gcaggatcag atccgccgcc agggccgaca ccgtcgggta gcagtccgac acgacgatga
    17821 agttggccgg gttgcgaaag ccgggccaga tctcgccgtt gatgttgggc ccggcctgca
    17881 tgttgttggt ggtcgtggtc cagtagaagt tgagcttgcc gtccttcagg gcgcggcttt
    17941 gcgccacggc gtgcaggccc acccagtcgg ggatggtgcc ggcgggcagc ttccactctt
    18001 tctcggagat ctcgcggtgc ttggggttgg tgaccaccat gtcggcgggc aggcggtgcg
    18061 agaaggtacc cacctcgcgc gccgtgccgc aggccgaggg ctggccggtg agcgagaacg
    18121 ggctgttgcc gggctcggag atcttgccca ccagcaggtg cacgttgtag atcatgttgt
    18181 tgacccaggt gccgcgcgtg tgctggttga agcccatggt ccagaacgag gtcaccttgg
    18241 tcttggggtc ggcatagacc ttggccaggg cctcgagctt gtccttgggc acacccgaga
    18301 gctcgtgcgc cttgtccagc gtgtactcgg cgacgaaggc cttgaactcg tcgaagctga
    18361 tgggcgccga ggcgtctggc gagcccttgg gcttgccgtc cgcgccggga tagccgttgt
    18421 tgccggccac ctgctccagc gggtgattcg ggcgcaggcc gtagccgatg tcggtcacgc
    18481 ccttgcggaa gttgacatgc ttcttcacga agtcctcgtt caccgcgcca ctctggatga
    18541 tgtggttgca gatgtagttg aggatcgcca ggtctgattg cggcttgaag atcagttcgc
    18601 tgtcggccag ctcgcaggag cggtgcgtga aggtggacag cacatgcagc ttgacatgct
    18661 tggccgtcag gcgccggtcg gtcacgcgcg accagaggat ggggtgcatc tcggccatgt
    18721 tcgcgcccca gagcacgaag gcgtcggcat gctcgcagtc gtcgtagcag cccatgggct
    18781 cgtcgatgcc gaaggtgcgc atgaagccgg ccacggccga ggccatgcag tggcgcgcat
    18841 tcgggtcgat gttgttggag cgaaaacccg ccttgaacag cttggaggcg gcatagccct
    18901 cccagatggt ccactggccg gagccgaaca tgccgatgtt gcgcggcccg cccgccttca
    18961 gcgcggcctt gcatttgtcc tccatgatgt cgaaggcctg gttccagctg atgcgcgtga
    19021 actcgccgtt cttttcgaac ttgccgtcct tcatgcgcag catgggctgg gtcaggcgat
    19081 ccttgccgta catgatcttg gagaggaagt agcccttgat gcagttcagg cccttgttca
    19141 ccggcgcgtc cgggtcgccc tgggtggcga ccacgcgccc gtccttgacg ccaatcaata
    19201 cgccgcagcc ggtgccgcag aagcggcagg cgcccttgtc ccagcgaata tcgtcggcgg
    19261 cgccggccga ctgcgccaat gcctgcgcac cgggaacgct cacgccaatg gccgtggcgg
    19321 cagcggtgac cgcatgcgcc ttgatgagtt ctcgacgggt tatttgcatt tggctaactc
    19381 ctggtcagga tgggtttctg tctgctggaa aatcatggcg atattcagga catcgtccat
    19441 gcgcgcgatg gcctcatagg tgtccatggc gccgcgatcg ttctccgcct ctatggtgac
    19501 gaccagtctg ccgtcctctg cagcggcgtg aacctccacg ccgggaatat gcgggagttg
    19561 tgcgcgtacc gtggcatccg tgcccggtct ggcgtaaacg atggcgctgg agatattcaa
    19621 gatactgcct tcaatcaaat acggtttcgg atattgccaa taccccggag ttaatcgaat
    19681 gacaagtatg cggccccatt tgtagaacgg gtcgtgcatg gcaggcgatg cgccgggaag
    19741 tggatgtccg agtaaaacgg tcggggatta tcgtgggagc cgtaagttca ttgttttttt
    19801 gacgatctaa tggtgaaccc ttattcgggg ttggcatttg agaaaaatca aatccacgat
    19861 gcgagtggca tgaatcgcac cgcccgtccg gccctgttgc ggtgcgggcg gccctgggcg
    19921 gtcgcgataa tgcggctccc tctgcccttg tgaaccgccc cgcccatgac caccgccatc
    19981 ctcagcgccc tgcccgaaga gcagggcggc ctcgtccaag ccctgcaggc gcccgaatgc
    20041 atcacccatg ccgggcgcag cttctggcgc ggccggctgc tgggccagcc ggtgctgctg
    20101 gcgctgtcgg gcataggcaa ggtggcggcg gccaccacgg cgacgctgct ggccgagcgc
    20161 tttggcgtgg cgcgcatcgt cttcaccggc gtggccggcg gcttgggcga tggcgtgcag
    20221 gtaggggatg tggtggtggc gcaggacttt ctgcagcacg acatggacgc ctcgcccatc
    20281 ttcccgcgct gggaggtgcc cggctacggg ctggcgcgca tgccctgcga tgcggcgctg
    20341 accactatgc tatttgaagc tgccagcgct tatctgacaa gcgatagagc cggatttgat
    20401 cccaacaatc cggcgattgc gcaccgccac gcagcgtgcg cgcaccaggg tttgatcgtc
    20461 agtggcgacc gctttgtctc cacctcggcc gaatccgccg cactgcgcca ggcgctgggc
    20521 gccgccggcc atcaggtgct ggccgtggag atggagggcg cggccgtggc ccaggtgtgc
    20581 cgcgactacg gcctgccgtt tgcggccgtg cgcaccatct ccgaccgggc cgacgacagc
    20641 gcgcatgtgg actttccgca tttcgtgcgc catgtggcca gccgctatgc cgatcacatc
    20701 atccagggtt tgctgcgcgc catgccgggt ggacgttgag cgttgggcgt attcagtgcc
    20761 agccgtcatg cgttcacgct caacgcttaa cgccaaacgc tacttcaacc agccgcgctt
    20821 gcgaaaatac agcatgggca ccacggcgct ggcgatcatc aggcagaggg cgtagatgta
    20881 gccatgctcc cattccagct cgggcatcag gcggaagttc atgccgtaga cgctggcgat
    20941 cagcgtgggc ggcagcaggg cgacgctggc caccgagaag atcttgatga tcttgttctg
    21001 gttgatgttc aaaaaaccga cggtggcgtc catcaggaag ttgatcttgt cgaacaggaa
    21061 ctgcgtgtgg ctgtccagcg actcgatatc gcgcaggatt tggcgcgcgt cctcgaactg
    21121 ctcggcgttg agcatcttgc tgcgcatcat gaagctgacg gcgcggcgcg tgtccagcat
    21181 gttgcggcgg atgcggccgt tcaaatcctc ttgccaggcg atgtcggcca gcaccttctg
    21241 cgcgtggtcg tcggtcacat ggccctccag cacctgggtg ctgaccttct tcagatcgtc
    21301 gtagatgccc tccagcgtgt cggccgaata ctccacgtcg gtgtcgaaca gcgacagcag
    21361 cacctccttg gcgtcctcga tcagccccgg cgcgcggcgc gcacgcatgc gtagcagtcg
    21421 gaacacgggg atgtcttcct cgcggatgga aaacagcacg ccgcagcttt tcagctcggt
    21481 gttgtgctgg ttcaggatga aggccacgcg caccgagcgc ggctcctcgt cgtcgtccac
    21541 cagaaagtcg ctgcggatat gcagttcgcc gttgtcttcc tcgtagaagc gcgccgactc
    21601 ttcgatgtcc tcgtccatca cgtccagcgg gatggccagg ccgtaatgct gcttgaccca
    21661 gcgcttttcc agcaccgtgg gcgactccat gtccacccag atcggatgct gcggcgcctg
    21721 atcctccagg gaatcgattt cttcctggac caggcggccg ttgacgagtg aaaagatgtt
    21781 gagcatggct cactctccct gttgttcttg aggcgtctgc gaggggtgat ggggggcgct
    21841 ttcagcccct cgtcagaccg gggagaggct gaaagctacc aactggggta ggtttccacg
    21901 ggtgggcggt gcgtgtgttg cgaagccggc gattatcgca ttcgcgtcgt gaccgcgctg
    21961 tgaccgcgcg tttgccccca ggtggtttca agccccgggc tggcgacgca ccagggccag
    22021 cgcgcggcac aggtcgatcc aggcggcggc cttggcttcc ggggcgcgca gcagataggc
    22081 cgggtcgtag ctgaccaccg caggcgtgcc gtccgccagc tggtgcgcgc tggcgcgcag
    22141 gcgccccagc ggctcgcggc tgcccagtac cacgcgcgcc gcgcccaggc ccagcaccag
    22201 caccatggcc gggcgcagcg tggtcagggc ctgctgcagg ccggcggccg gcggttcgcc
    22261 ctcggccggc cggcccggct ggggctgtga cagggtggcg acgaagcagc gcgggtgccg
    22321 gtgcaggcgc atggcgcgca gcatgttgtc cagcagctgg ccgctgtcgc ccgccagggg
    22381 ttcggccggt gtcgggctct ccagcagcac cagccagccg gagcccagtt ccggcggcgt
    22441 ggccgtcggg tcggcctgcg ggtacagggc tatgggtggg cgcagcagca gtccgccggc
    22501 ctgggccatg gctgcagccg gggttggcgc ggcagcttgc gtcggtgcct ggcggcgcgt
    22561 ggtggggggg ggcgctgcgg cagcttgcgc tgcgggacgg gccggggtgg cggcgggtgc
    22621 ggggcgagtg acgggagcgg cctcgttggt cggcggcgcc gctggcgcgg cggccggggc
    22681 cggggcccag acggtgacac ccatctccag cagcatggcg cgttggcgcg cgtccagttc
    22741 caggctcata acggcagact catgaccacg gcgtcctcgc gttcgccatc gctggacggg
    22801 tagtagcgct tgcgcaggcc cacctgctgg tagccatggg cctcgtacac ctggcgtgcg
    22861 cgcgtgttgc tggcgcgcac ctccagccac agccactgtg cgccctggcc gcgtgcccac
    22921 agggccagcg cgtccagcat cacatgcgcc cagccctggc gctggaacgc cggggccacg
    22981 gtgatgttga gcaaatgggc ctcctgcacc ccgggcatgg caacgaagta acccaggatc
    23041 tgcccgcccg ccaccagcag ctgcgcctgg tagccggcgg ccagcgcgtc aatgaagttg
    23101 ccgcgcgtcc acgggtggct gtaggcggcc tgctccacgg ccagcagcgc gtccaggcgc
    23161 tctgtcgtga gcggcgcaaa agacgcctgc atggcgtcag cggggcgcgg gttggcgttc
    23221 atgatgcttg cgccgcgctg gcgcggcgcg cggcctcgcg ctcggccgtg gtttgggcca
    23281 ccttgtcgcg tatgtagcgc ggcagcgccg cgtcggccgg cacggccctg ccggcggcca
    23341 gcagcgcggg tgcca
//
LOCUS       DAOMTQ010000029        23157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_237_length_23157_cov_26.315644, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000029 DAOMTQ010000000
VERSION     DAOMTQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 23157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23157
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_23157_cov_26.315644"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PK518_04715"
     CDS             <1..788
                     /locus_tag="PK518_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HRM48246.1"
                     /translation="IAQAAEDDTNIHLRDWSLVRQATTAPGASRYQIRAQADGFALEL
                     DCTSTQPILLQGDAGLSRKGPDASQASYYYSQPQLAVTGAITLGGRRMTVTDAPGPDN
                     RAWLDHECSQALMPPDAVGWDWIGMNLEDGGALTAFQLRRADGSALWAGGSWRQAGAS
                     HAQAFGPGAVRFTPLRRWHSASSGTLYPVEWRVDTPAGSFTVRALLDAQELDSRGSTG
                     AIYWEGLSQLRDAAGHALGRGYLEMTGYAQPLRLVRQPGNIET"
     gene            857..1609
                     /locus_tag="PK518_04720"
     CDS             857..1609
                     /locus_tag="PK518_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM48247.1"
                     /translation="MNKRRHIPLSMPDPDPQACSLAMRLAVCQKVPLFAELDAQQLAQ
                     INRHCRAQRFDAATPVYMEGDAATHLYVVATGAVKTTRLAADGRESLIDLLTPGDFFG
                     ALPALGQKHYADSATTLTPACLLGLDASEYDAVMHEIPQVAVATLKGVAHRLTQSQQA
                     IHMLAGAPLEQRLAAILLVLAGKVGKPWNGATLLDVPLAREDLAAMAGAATESVSRLL
                     SQWQREGAIEAGRRWVAVADAARLRQLRDGMG"
     gene            1723..1953
                     /locus_tag="PK518_04725"
     CDS             1723..1953
                     /locus_tag="PK518_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRM48248.1"
                     /translation="MNPTNTRTTLRAEGFSCPSCVTKIEKSVGGMAGVSQVKVHFASA
                     RIEVDHHPETVSVDDLIAAIAKVGFTARASAF"
     gene            2071..3984
                     /locus_tag="PK518_04730"
     CDS             2071..3984
                     /locus_tag="PK518_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM48249.1"
                     /translation="MNNINAWINGRWTIPALSGLLILASFTASKGYGAATAGDLLMVA
                     AAIVAGLPVVRNAIRALAVRHISIDLLVSIAAIGALIIGEYWEAAAVTFLFAIGHALE
                     SATLNKTRAALAELIAVAPDTAIVMRKGEQVEIPAASVAMGEVVLVKNGAKVPVDGEV
                     IAGTGALDEASITGESIPVEKSKGDHVFAGTISRGGFLQVLATGIGADTTLARIIHRV
                     EDAQDAKARTQKFIDKFSSWYTPAIVVLALLAGLISGSVVLGLTLLVVACPGALVISI
                     PVSIVAGIGRSARDGILIKGGEFLETAAKIDVVAVDKTGTLTKGRPQLTDVVALAPGV
                     EKNQALAWAARAEAGSEHPLARPILEAAEKAGIPVAALPEHIEPVIGKGITARVDGKR
                     VLIGNLALLEQFGVADTVGADRVAHELATQGRTPMIVAVDDQVLGVIAVADEIRRDAA
                     AMVAALHKAGVKKVVMLTGDVKPVADAVAAATGIDEVHAGLLPEDKLDIVARLQRAGH
                     VVAMVGDGVNDAPALATADVGVAMGAAGSAVAVETADIALMGDDLLKLPEAISLARRT
                     LANIRQNIVIALVTVGLLLAGVLMGGVTMAAGMLFHEGSVLIVIANAMRLLRRKTPAR
                     ANLPGPLRQEIRR"
     gene            complement(4102..5103)
                     /locus_tag="PK518_04735"
     CDS             complement(4102..5103)
                     /locus_tag="PK518_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM48250.1"
                     /translation="MNVKKFAPIGYGLLLLGLIAAPFVGAYPVFVMKLLCFALFATAF
                     NLLLGYTGLLSFGHAAFLGGAAYVTGHAVKVWGATPELGLLIGTLSGALLGLVMGWFT
                     IRRQGIYATMITLALAQMLFFAALQMPFTGGEDGLQGVPRGKLFGVLDLSSDLTMYYV
                     CLVIVVLAFLLIVRTIDSPFGQVLRGIKENEPRATSLGYDTNRFKLLAFVISAAIAGL
                     AGALKTLVLGFATLSDVHWSASGHVVLMTLVGGMGTLAGPLVGAAVVVALENKIGDLG
                     TLLAKLTTIDWFNTLGESVTIVTGLIFVVCVLAFRRGIMGEIVAWLERRGTGGGNSH"
     gene            complement(5123..6007)
                     /locus_tag="PK518_04740"
     CDS             complement(5123..6007)
                     /locus_tag="PK518_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM48251.1"
                     /translation="MEIFGVSMPAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINF
                     AHGALFMLGALITWMAMSYLGVNYWVMLIASPLVVGALGVVIERLFLRWIYKLDHLYG
                     LLLTLGLTMVIEGVFRSIYGVSGLGYDTPELLEGATNLGFMILPNYRAWVVVASIVVC
                     IATWYVIEKTKLGAYLRAGTENPRLVEAFGVNVPVMITLTYAFGAALAAFAGVLAAPV
                     YQVTPLMGQNLIIVVFAVVVIGGMGSIMGSILTGLGLGVIEGFTKVFYPEASSTVVFV
                     IMAIVLLIRPAGLFGKEK"
     gene            complement(6052..7248)
                     /locus_tag="PK518_04745"
     CDS             complement(6052..7248)
                     /locus_tag="PK518_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48252.1"
                     /translation="MKTNYTALVLMVAAAGLAGTAAQAQDKVKIGYISDMSSLYADLE
                     GKHGATAIQMAIDDFGGKVLGMPIELMSADHQNKADIAASKAREWIDTQGLTMIFSGT
                     NSGTALALAKIAEEKKRVLINNGAASSALTNDQCTPYTVHYAYDTVALSKGAAGAIVE
                     GGAKTWFFLTADYAFGHAMEADASKVVKDKGGTVVNAVRHPLNASDFSSFLLQAQNSK
                     AQVLALANAGGDTINTIKAAKEFGIGKSMKVAPMLVFYSDVHSLGLKNTEGMQFVTSW
                     YWDMDDASRKFADAFMKKTNRRPTEIQAADYSATMNYLKAVQAAGTVDADKVMATWRG
                     MKMDDFFAKGQIRPDGRYVHDMYLVQVKSPAESKGTWDYYKIVKKLPGESVFTTKAES
                     KCALWK"
     gene            complement(7330..8532)
                     /locus_tag="PK518_04750"
     CDS             complement(7330..8532)
                     /locus_tag="PK518_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48253.1"
                     /translation="MNRKLKTLVAMLGAAGLGLVATQAQAQDKVKIGFITDMSSLYAD
                     VEGKNGALAIQMAIDDFGGKVLGKPIELLSADHQNKADIAASKAREWIDTQGLTMLFG
                     GTNSGTALAMAKIAEEKKRVFVVNGAGSSALTNDQCTPYTVHYAYDTTALAKGTGGAV
                     VKQGGKTWFFLTADYAFGAALEADTAKVVKENGGQVLGQVKHPLNASDFSSFLLQAQN
                     SKAQILGLANAGGDTINAIKAAKEFGISKTMKMAGLLVFITDIHSLGLKNTEGLLLTT
                     SWDWNLNDKTREFGKKFFAKTKRMPTDIHAADYSATMNYLKAVQAVGTTDADKVMAKL
                     KSTPIDDFYAKGVIRPDGRFAHDMYLMQVKSQADSKEPWDYYKVVAKLPADQVWTTKA
                     ESKCALWK"
     gene            complement(8595..9308)
                     /locus_tag="PK518_04755"
     CDS             complement(8595..9308)
                     /locus_tag="PK518_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48254.1"
                     /translation="MAGTPALEIKGLEAWYGESHVLHGVDMVVQPGEVVTLLGRNGAG
                     RTSTLRAIMGLTGARKGSIKVYGTETIDMATHRIAHLGLGYCPEERGIFSSLSCEENL
                     LLPPALKTGQAGMSVDEIYAMFPNLAERKNSQGTRLSGGEQQMLAVARILRTGAKLLL
                     LDEISEGLAPVIVQALARMITTLRAKGYTVVMVEQNFRFAAPLADRFYVMEHGRIVER
                     FEASELQAKMPVLNQLLGV"
     gene            complement(9310..10083)
                     /locus_tag="PK518_04760"
     CDS             complement(9310..10083)
                     /locus_tag="PK518_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48255.1"
                     /translation="MSDDVILKTSHLTKEFKGFTAVSDVNLSVRRGSIHALIGPNGAG
                     KTTCFNLLTKFLVPTSGSIVFNGTDITNEQPAQIARRGVIRSFQISAVFPHLTLMENV
                     RLGLQRKLGTSFHFWRSQSSLNQLNDRAVQLLAEVGLEEMADEVTVNLPYGQKRALEI
                     ATTLGMEPELMLLDEPTQGMGHEDVDRVTQLIKKVSAGRTILMVEHNMKVISTIADRI
                     TVLQRGAVLAEGNYEEVSNNPQVMEAYMGTTDGELQGAH"
     gene            complement(10248..11021)
                     /locus_tag="PK518_04765"
     CDS             complement(10248..11021)
                     /locus_tag="PK518_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM48256.1"
                     /translation="MPPMQQSESLVNALGHGMVDKRIDILRQIGQGGSISQAARAAGV
                     SYKAAWQALDTLTNLAGVTLVERVVGGAGGGGARLTPAAEQLLAAADAVRQARVSALA
                     QLEGGGADLAVARLSLRTSMRNQWPCIVQDMRSCGQIARVHLCGVGPAAAGLAVTARI
                     TLESAELLGLHAGVPVQALCKATAVHVLAATKAPVPAGGNQWPGRASRVSRGELGDEV
                     AVQLDAGVQMVGFAAPDSGLRARSRVVLVIDESAVVLAV"
     gene            11090..11833
                     /gene="modA"
                     /locus_tag="PK518_04770"
     CDS             11090..11833
                     /gene="modA"
                     /locus_tag="PK518_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM48257.1"
                     /translation="MHPLRSLALAAVLLPTAHAGEVSVAVAANFTAPMQKIAAQFEQD
                     TGHKAQLSFGATGKFYAQIANGAPFGVLLAADDTTPEKIGKEGLGDGSTRFTYAIGQL
                     VLWSKQPGYVDADGKVLQKTDWQHIAIANPKLAPYGLAAMQTLNRLGLTAQVQPRIVQ
                     GENIGQTYQFAASGNAQLGFVALSQVMEGGKLREGSAWVVPASMHESIRQDAIVLKPG
                     QGNAAAAALMDYLKGDKARAIIKSYGYSF"
     gene            11859..12539
                     /gene="modB"
                     /locus_tag="PK518_04775"
     CDS             11859..12539
                     /gene="modB"
                     /locus_tag="PK518_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRM48258.1"
                     /translation="MPLLSPEDWSAIRLTLELAGITTVLLLVLCTPIAWWLAHTPSRW
                     RGPVGAVVALPLVLPPTVIGFYLLVLMGPSGPVGRLTESLGWGRLPFTFWGLVVGSIV
                     YSLPFAVQPLQRAFESMGRRPLEVAAALGAGPLDRFFTVALPLARPGFITAAVLSFAH
                     TVGEFGVVLMLGGNIPGKTRVVSVQIYDHVEAMEYAQAHWLAGGMVAFAFVVLMALHW
                     LQPKGRLQ"
     gene            12536..13612
                     /gene="modC"
                     /locus_tag="PK518_04780"
     CDS             12536..13612
                     /gene="modC"
                     /locus_tag="PK518_04780"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HRM48259.1"
                     /translation="MTGINARLRLQRPDFLLDVDLQLPSQGVTALFGPSGCGKTTCLR
                     AIAGLERTTGRVEVNGQPWQDDAAGLWLPTHKRQLGYVFQEASLFAHLNVRQNMEYGL
                     RRLAPERRRVSLDQAVELLGLSAMLARMPHTLSGGERQRVAIARALAASPRLLLMDEP
                     LAALDAARKAEVLPYLERLQRSLDIPMLYVSHSPDEVARLASHLVLLQAGHVKVQGPT
                     AELMTRLDLPLSHGDTAAALVQAQVLAHDAHDHLLSVAFSGGRLHLIATQQYVAGEAV
                     RLRVLARDVSLARTRATDSSILNLLPARITQLAEDSPGQMLVALDAAGTALLARITRR
                     SCQQLALAVGDTVFAQVKGVALVD"
     gene            13669..14715
                     /locus_tag="PK518_04785"
     CDS             13669..14715
                     /locus_tag="PK518_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRM48260.1"
                     /translation="MNTKHAHLTPTEHIPFDTLRTTLAHIFERHGTSAAVAAVLADNC
                     ASAERDGAHSHGVFRIPGYLSTLASGWVDGHAIPRVRDMAPGFLSVDACNGFAQPAYM
                     MAYDALVAKARANGIALLAIHNSHHFAALWPDVEPLARDGLVALALVNSMACVVPSGG
                     AKALFGTNPIAFAAPRAGHDPLVFDLATSAMAHGDVQIAAREGRPLPEGFGVDRHGQP
                     TTDPRAVLDGGALLPFGGVHSSYKGSALSMMVELLAAALTGGHFSFEFDWSGHPGAHT
                     PHTGELIIAIDPAKNQGRDFAQRCEDLVRAMAGAGQQRLPGERRYRQRAQSLREGVAL
                     PAATWAELQRLAQA"
     gene            complement(14771..16423)
                     /locus_tag="PK518_04790"
     CDS             complement(14771..16423)
                     /locus_tag="PK518_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRM48261.1"
                     /translation="MTANHMYDQNLSRNAANHAPLSPLSFIERTAEVYPERLAIVHGE
                     LRQTWAQTYARCRRLASSLQRAGIAKNDTVAVMLPNTPPMVEAHFGVPMAGAVLNALN
                     TRLDPETIAFMLDHGEAKAVIVDPEFATVIAKALTLRQSKAPLLLIDVEDEVYGAAEL
                     RVGQQTYEEFLAAGDPQFAWELPGDEWDAIALNYTSGTTGNPKGVVYHHRGATINAIS
                     NVLEWDMPKHAVYLWTLPMFHCNGWCFPWTVAARAGVNVCLRRVEAQAIFDAMRKHGV
                     THYCSAPIVHGLLVNAPAAMKAGVPAGIKAMVAGAAPPASMIEGMEKLGFDLTHVYGL
                     TEVYGPATVCAKHDAWGDLDIGERARLNARQGVRYHLQRAAAVLDPETMQSVPHDGET
                     MGEIMFRGNIAMKGYLKNPQATEEAFRGGWFHSGDLAVQYPDGYIKIKDRSKDIIISG
                     GENISSIEVEDVLYRHPDVLAAAVVARPDPKWGETPCAFVELKAGAQTTPEDIVAHCK
                     QHLAGFKVPRAVVFGELPKTSTGKIQKFELRKQAGSAAAINV"
     gene            16521..18467
                     /gene="dnaG"
                     /locus_tag="PK518_04795"
     CDS             16521..18467
                     /gene="dnaG"
                     /locus_tag="PK518_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRM48262.1"
                     /translation="MSIPQSFIQELLARVDVVDIVGRYVQLKKSGANFMGLCPFHGEK
                     SPSFSVSPSKQFYHCFGCGKNGNAIGFLMDHAGMGFVEAVQDLAQQTGMQVPQDDQSP
                     QDRERAAAQRQKQATLTDVLEKAGEAWRKHLRDSPRAIQYFKGRGVSGQIASRYGLGY
                     APEGWRGLASVFPEYDSPLLEESGLVIVNAEDGKRYDRFRDRVMFPIRNVKGECIGFG
                     GRVLGDDKPKYLNSPETPVFHKGRELYGLFEARQAIREMGYALVTEGYMDVVALAQLG
                     FANAVATLGTACTPDHVHKLFRFTEQVVFSFDGDAAGRRAARKALDGALPYASDTRSV
                     KFLFLPSEHDPDSFIRAHGPEAFARHVGEAMPLSRFLLEAAREGCDLGQAEGRAHMLS
                     NARPLWSALPDGALRRQLLAELGELGQLPATELASLWAQDGARHPGPAGAAHNAPPFG
                     DEPPWGQEPPGSWPGDTAQTTRPWRKSGDWKGKGGRWRREEQRPAQPPLPRTPVAGRA
                     DHAARLLLSHMQFLEELSHEDHGLLREQPAPHGALFVWLEGQLHEHGPQSWAVLREAL
                     AGHDSQALAERLMSGSHAQTEGDEAELRAELRGLLNRMLIDALKQEQNALIAAAAQDP
                     TALQRYRQLQERRRILESHGQPGS"
     gene            18727..21141
                     /gene="rpoD"
                     /locus_tag="PK518_04800"
     CDS             18727..21141
                     /gene="rpoD"
                     /locus_tag="PK518_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRM48263.1"
                     /translation="MPVQKSAKSPKSATGTAAQAASEATTTVKTAAKAPAKAAKSKAA
                     AAPEATTKVKTVPTPKSSAELEKAADELLKKKPARAAKAAPAEDAQEAPKKKPGRKPK
                     AADDEATAPKAAAKKAPAKRGRKPKGKEEGLEDDADLSDIEADLEGEPEPEVSTTEAA
                     PAEKVKPLRMKISKAKERALMKEFGLDETVLSEEDMAKRRAQLKKLITLGKTRGYLTQ
                     VEISDHLPDKLVDAETLEVVISMLSDMGVAVYEQTPDAETLFQNNVTPTATTVEEAEE
                     EAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLMDMMEAISA
                     SPATIAEILNMGEEIREGKVIINTVVDGFSNPEEADDYVAEEDFDEYDEEDDDDGKGG
                     SKAMTRLLEKLKNDALERFDELRALFEKMHKIYDKEGYGTPAYMKVQHEISAKLMTIR
                     FTAKTIEKLCDMVRAQVDDVRKKERELRRIIVDKCGMPQETFIKEFPPNLLDRTWVEK
                     QAAAAKPWSTVMQRNIPPIQELQQHLIDLQSRVVVPLGELKAINKRMNQGESNSRDAK
                     KEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWW
                     IRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQEFGFEPDASILAEKMEIPED
                     KIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDSNNTAPVDAAMQAGLRDVVKDILDG
                     LTPREAKVLRMRFGIEMTSDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLR
                     SFIDSL"
     gene            21263..22195
                     /locus_tag="PK518_04805"
     CDS             21263..22195
                     /locus_tag="PK518_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HRM48264.1"
                     /translation="MTTTPDTSAPRLVFAVMGAGSVGCYFGALLARAGHTVTLIGRQA
                     HVQAITAHGLRLQSATLDEHVRLSASTEAKAVAGADVVLLCVKSTDTETAARQIQPHL
                     APGALVLTLQNGVDNDERVRAMLGPAQPVAAAVVYVATAMAGPGHVRHFGRGDLLIAP
                     SPVSERLALELAAAGIPTQVSGNVRGALWAKLVINCAYNALSAITQQPYGWLVQQDGA
                     SAVIADLVAECQAVAQADGVRMDGDVHAAVRGIAQSMPGQLSSTAQDLARGRPSEIEH
                     LNGYVVRRGAALGLATPVNRALLVLVRMLQAKSI"
     gene            complement(22251..>23157)
                     /locus_tag="PK518_04810"
     CDS             complement(22251..>23157)
                     /locus_tag="PK518_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRM48265.1"
                     /translation="PGVMAKYGLDYAALSRLNERMVYVNHTGFLPGPYEHRTALDEVV
                     QMMGGLAYMTGRSGDPLRAGSSVNDIMGGMFGAMGAMAALMQRQQTGLGQEVDSALFE
                     NNVFLVGQHMMQYAVTGQPAAPMPERISSWAVYDVFDVKDGGQIFLAVVSDAQWQVFC
                     DAMGYADLKADPRHASNNDRVRQRPTLVPELRRRLGQHRAEELAAVFERHGLPFAPIA
                     RPEQLFDDPHLTATGGLAEITLPDGERAGQVARTTLLPLRMDGRRLAVRSDPPRLGQH
                     TEPLLQGLGYDAGQIQALLEAKAVV"
BASE COUNT         4091 a         7819 c         7494 g         3753 t
ORIGIN      
        1 gcatagcgca ggctgccgag gacgacacca acatccacct gcgcgactgg tcgctagtac
       61 gccaggccac gaccgcgccg ggcgccagcc gctaccagat tcgcgcccag gccgacggtt
      121 ttgcgctgga gctcgactgc accagcaccc agcccatcct gctgcagggc gacgccggcc
      181 tgtcgcgcaa ggggcccgat gccagccagg ccagctacta ctacagccag ccgcagctgg
      241 ccgtgacggg ggccatcacc ctgggcgggc gccgcatgac cgtgaccgac gcgcctgggc
      301 ccgacaaccg cgcctggctc gatcacgagt gcagccaggc gctgatgccg cccgacgccg
      361 tgggctggga ctggatcggc atgaacctgg aagacggcgg cgcgctcacc gccttccagc
      421 tgcgccgcgc cgacggcagc gcgctatggg cgggcggatc ctggcggcag gcgggtgcat
      481 cccatgcgca ggccttcggg ccgggggcgg tgcgcttcac gccgctgcgg cgttggcaca
      541 gcgccagcag cggcacgctc tacccggtcg aatggcgcgt ggacacgccg gcaggcagct
      601 ttaccgtgcg ggcgctgctg gatgcccagg agctcgacag ccgcggctcg acgggcgcga
      661 tctactggga aggattgagc cagttgcgcg acgcggcggg acatgcgctg gggcgcggct
      721 atctggaaat gacgggctat gcgcagccgc tgcgcctggt acggcaaccc ggaaatatcg
      781 aaacatgaaa gcgccacctt cagtcatgcc tctgcatacg accaagagcc cttcaggagc
      841 aagagaaata caggccatga acaagcgtcg ccacattcca ctgtccatgc cggaccccga
      901 cccgcaggcc tgctcgctgg ccatgcgcct cgccgtctgc cagaaagtac cgctctttgc
      961 cgaactcgac gcgcagcagc tggcgcagat caaccggcat tgccgggcgc agcgctttga
     1021 cgccgctacg ccggtctaca tggagggcga tgccgcgacg catctttacg tggtggcgac
     1081 cggtgccgtc aaaaccacgc gccttgcggc cgatgggcgc gagtccctga tcgacctgct
     1141 caccccgggc gacttttttg gcgccctgcc ggcgctgggg caaaagcact acgcagacag
     1201 cgccaccacc ttgacgccgg cctgcctgct cgggctcgat gcatccgaat acgatgccgt
     1261 gatgcatgaa atcccgcagg tggccgtggc caccctgaag ggggttgcgc accggctgac
     1321 gcagtcgcag caagccatcc acatgctcgc cggcgcgccg ctcgagcagc ggctggccgc
     1381 catcctgctg gtgctggccg gcaaggtcgg caagccctgg aacggggcga cgctgctgga
     1441 cgtgcccctg gcgcgcgaag acctcgctgc catggccggc gcggccaccg aatcggtcag
     1501 ccgcctgctg agccagtggc agcgcgaggg cgctatcgag gcgggccggc gctgggtcgc
     1561 cgttgccgat gcggcgcgcc tgcggcagct gcgcgacggc atgggctgac cggcacgcta
     1621 aggccgcgtg cggttgattg acgtttctca tggagccatc cggccggccg gcctatggtg
     1681 aaggcatccc ccaaccaagt agtcatcaac aaggagaaca ccatgaaccc cacgaacacc
     1741 cgcaccacct tgcgcgccga aggcttttcc tgcccgtcct gcgtgacaaa aatcgagaaa
     1801 agcgtcggtg gcatggctgg cgtgagccag gtcaaggtgc acttcgcgtc ggcgcgcatc
     1861 gaagtcgatc accaccccga gaccgtcagc gtggacgatc tcatcgccgc catcgccaag
     1921 gtcggcttca cggcgcgggc atcggcgttc tgacgcggcg cagcgggccg cagcatggcc
     1981 cgctcatttc accccacagc agcatgccgc ctggcggcgg catgcctctt cttttgatct
     2041 cccgaccctg atcacgaaca tggatacctc atgaacaaca tcaatgcatg gatcaacggc
     2101 cgctggacga ttcccgccct gtccggcctg ctgatcctgg cttcattcac cgcctccaag
     2161 ggctacggcg ccgccacggc cggcgatctg ctgatggtcg ccgccgccat cgtggccggc
     2221 ctgcccgtcg tgcgcaacgc catccgcgcg ctcgccgtgc gccacatcag catcgacctg
     2281 ctggtgagca ttgccgccat cggcgcgctg atcatcggcg agtattggga ggcggcggcc
     2341 gtcaccttcc tgtttgccat tggccacgcg ctggaatcgg ccaccttgaa caagacccgc
     2401 gcggcgctcg ccgagctgat tgccgtggcg ccggacacgg ccatcgtcat gcgcaagggc
     2461 gagcaggtcg aaatccccgc cgccagcgtc gccatgggcg aggtggtgct ggtcaagaac
     2521 ggcgccaagg tgccggtcga tggcgaggtg atcgccggca ccggcgcgct cgacgaggcg
     2581 tcgatcaccg gcgaatccat cccggtggaa aagtccaagg gcgaccatgt cttcgccggc
     2641 accatctcgc gcggcggctt cctgcaggtg ctggccaccg gcatcggcgc cgacaccacg
     2701 ctggcgcgca tcattcaccg cgtggaagac gcgcaggacg ccaaggcgcg cacgcagaaa
     2761 ttcatcgaca agttttcgtc ctggtacacc cccgccatcg tcgtgctggc gctgctggcc
     2821 gggctcatca gcggcagcgt ggtgctgggc ctgaccctgc tggtggtggc ctgcccgggg
     2881 gcgctggtga tctccattcc cgtgtccatc gtcgccggca ttggccgctc ggcacgcgac
     2941 ggcatcctga tcaagggcgg cgagttcctg gagaccgcgg ccaagatcga cgtcgtggcc
     3001 gtggacaaga ccggcacgct gaccaagggc cggccacagc tcaccgacgt ggtggcgctg
     3061 gcgccgggcg tggagaagaa ccaggcgctg gcctgggccg cgcgcgccga ggcgggctcc
     3121 gagcatccgc tggcgcgccc catcctggaa gccgccgaga aggccggcat cccggtggcc
     3181 gcgctgcccg agcacatcga gccggtcatc ggcaagggca tcaccgcgcg cgtcgatggc
     3241 aagcgggtgc tgatcggcaa cctggccttg ctggagcaat ttggcgtcgc cgatacggtg
     3301 ggcgcagacc gcgtggcgca cgaactcgcc acccaggggc gcacgcccat gatcgttgcc
     3361 gtggacgacc aggtgctggg cgtgatcgcc gtggccgacg agatccggcg cgacgccgcc
     3421 gccatggtgg ccgcgctgca caaggcgggc gtgaagaagg tcgtcatgct caccggcgac
     3481 gtgaagccgg tggccgatgc cgtcgccgcc gccaccggca tcgacgaggt gcacgccgga
     3541 ctgctgcccg aggacaagct cgacatcgtc gctcgcctgc agcgcgccgg ccatgtcgtg
     3601 gccatggtcg gcgacggcgt caacgacgcc ccggcgctgg ccaccgccga cgtcggcgtg
     3661 gccatgggcg cggccggctc ggccgtggca gtggaaaccg ccgacatcgc gctcatgggc
     3721 gacgacctgc tgaagctgcc cgaggccatc tcgctggcgc ggcgcacgct cgccaacatc
     3781 cgccagaaca tcgtgattgc gctggtgacc gtcggcttgc tgctggccgg ggttctcatg
     3841 gggggcgtga ccatggccgc cggcatgctg ttccatgaag gctcggtatt gatcgtcatc
     3901 gccaatgcca tgcgcctgct gcggcgtaaa accccggcgc gcgccaatct gccaggcccg
     3961 ctgcgccaag agatccggcg ttgatcaatc cgagcgattg attgatggtt gcttgccatg
     4021 aaaacacagc ggccatacgc ggcaaggtac cgtgtatggc cgctgcgcgg ggcatggcgg
     4081 cagggccatg catgcctcac gtcaatggct gttgccgccg ccggttccgc gccgctccag
     4141 ccaggcaacg atctcgccca tgatgccgcg gcggaaggcc agcacgcaga ccacgaagat
     4201 caggccggtg acgatggtca ccgactcgcc cagggtgttg aaccagtcga tggtcgtcag
     4261 cttggccagc agcgtgccca ggtcgccgat cttgttctcc agcgccacca ccacggcggc
     4321 gccgaccagc gggccggcca gcgtgcccat gccgccgacc agggtcatca agaccacatg
     4381 gcccgaggcg ctccagtgca cgtccgacag cgtggcaaag cccagcacca gggtcttgag
     4441 ggcaccagcg agcccggcga tggcggccga gatcacgaag gccagcagct tgaagcggtt
     4501 ggtgtcatag cccagcgagg tggcgcgcgg ctcgttttcc ttgatgccac gcagcacctg
     4561 gccaaacggc gaatcaatgg tgcgcacgat gagcaggaag gccagcacca cgatcaccag
     4621 gcacacgtag tacatggtca gatcgctgga cagatcgagc acgccgaaca gcttgccgcg
     4681 cggcacgccc tgaaggccgt cctcgccacc ggtgaacggc atttgcagcg ccgcaaagaa
     4741 cagcatctgc gccagcgcca gcgtgatcat ggtggcgtag atgccctggc ggcggatggt
     4801 gaaccagccc atcaccaggc ccagcagtgc accagagagc gtgccgatca acagacccag
     4861 ctcgggtgtg gcgccccaga ccttgacggc atggcccgtc acataggccg cgccgcccag
     4921 aaaggccgca tggccaaagg acagcaggcc ggtgtagccc agcagcaggt tgaaggccgt
     4981 ggcgaacagc gcgaagcaca gcagcttcat cacgaacacc gggtaggcac ccacgaaggg
     5041 cgcggcgatc aggcccagca gcagcaggcc gtaaccgatg ggggcaaatt tcttgacgtt
     5101 catgtggatc acccctctcg gcttactttt ctttgccgaa caggccggcg gggcggatga
     5161 gcaaaacaat ggccatgatg acgaacacga cggtggaaga cgcctcgggg tagaacacct
     5221 tggtgaaacc ctcgatcacg cccagcccca gaccggtgag gatggagccc atgatggagc
     5281 ccatgccgcc gatcacgacc acggcaaaca ccacgatgat caggttctgg cccatcagcg
     5341 gcgtgacctg gtacacgggc gcggccagca cgccggcaaa ggccgccagc gcggcgccga
     5401 aggcgtaggt cagcgtgatc atcaccggca cgttgacgcc aaaggcctcg accaaacgcg
     5461 ggttctcggt gccggcgcgc agataagcgc caagcttggt cttctcgatc acataccagg
     5521 tggcaatgca caccacgatg gaggccacca ccacccaggc gcggtagttg ggcaggatca
     5581 tgaagcccag gttggtcgcg ccctccagca gctcgggcgt gtcatagccc aggccagaga
     5641 caccgtagat ggagcggaac acgccctcga tcaccatcgt caggcccagc gtgagcagca
     5701 ggccgtacag gtgatcgagc ttgtagatcc agcgcaggaa cagccgctct atgaccacgc
     5761 ccagggcccc gaccaccagg ggcgaggcga tcagcatcac ccagtagttg acacccaggt
     5821 aactcatggc catccaggtg atcaacgccc ccagcatgaa caaagcgcca tgggcgaagt
     5881 tgatcacgtt gagcaggccg aagatgacgg ccagacccaa actgaggatc gcgtagaacg
     5941 agccattgac cagccccagc aggagctggc tgagcatggc cggcattgaa acaccaaaga
     6001 tttccatgag aaaactgcgt gatgagagga aggggtcggg tcagccgtgc atcacttcca
     6061 cagcgcgcac ttgctctcgg ccttggtggt gaacaccgat tcgccgggca gcttcttgac
     6121 gatcttgtag tagtcccagg tgcccttgga ctcggctggg ctcttcacct gcaccaggta
     6181 catgtcgtgc acgtagcggc catcgggacg gatctggccc ttggcgaaga agtcatccat
     6241 cttcatgccg cgccatgtcg ccatgacctt gtcggcatcg acggtgcctg ccgcctgcac
     6301 ggccttgagg tagttcatgg tggccgaata gtctgcggcc tggatctcgg tggggcggcg
     6361 attggtcttc ttcatgaagg cgtcggcaaa cttgcgcgag gcgtcatcca tgtcccagta
     6421 ccagctggtg acgaactgca tgccctcggt gttcttcagg cccaggctgt gcacgtcgct
     6481 gtagaacacc agcatcggtg ccaccttcat ggacttgcca atgccgaatt ccttggccgc
     6541 cttgatggtg ttgatggtgt caccgccggc gttcgccagc gccagcacct gggccttgga
     6601 attctgcgcc tgcagcagga aagaggagaa gtccgaggcg ttgagcggat ggcgcaccgc
     6661 gttcaccacc gtaccaccct tgtccttcac gaccttggag gcatcggcct ccatggcatg
     6721 gccgaacgca tagtcggccg tcaggaagaa ccaggtcttg gcgccaccct cgacgatggc
     6781 accggctgcg cccttggaga gcgccaccgt gtcataggca tagtgcacgg tgtagggcgt
     6841 gcactggtca ttggtcagcg ccgacgaggc cgcgccgttg ttgatcagca cgcgcttttt
     6901 ctcttcggcg atcttggcca gtgccagggc ggtgccggag ttggtgccgc tgaagatcat
     6961 cgtcaggccc tgcgtgtcga tccactcgcg cgccttgctg gcggcgatgt cggccttgtt
     7021 ctggtggtcg gcgctcatca gctcgatggg catgcccagc accttgccac cgaagtcatc
     7081 gatggccatc tggatggccg tcgcgccatg cttgccttcc aggtcggcat acaggctgga
     7141 catatccgag atatagccga tcttgacctt gtcctgcgcc tgcgcggcag taccggccaa
     7201 gcctgcagca gccaccatca gcacgagtgc cgtgtagttt gtcttcatgt tgtactgtct
     7261 ccgaggtttc tttcaatcgc cacggcctgc gcggcaatgc gtcaggcagc ggcgggcttc
     7321 agctcaagct cacttccaca acgcgcactt gctttcggcc ttggtggtcc acacctggtc
     7381 tgccggcagc ttggccacga ccttgtagta gtcccatggt tccttcgagt cggcctggga
     7441 cttcacttgc atcaggtaca tgtcgtgggc aaaacgacca tccggacgga tcacgccctt
     7501 ggcatagaag tcatcgatgg gcgtggactt gagcttggcc atcaccttgt cggcatccgt
     7561 tgtgcccacg gcctgcacgg ccttgagata gttcatcgtc gccgaatagt cggccgcatg
     7621 aatatccgtg ggcatgcgct tggtcttggc gaagaacttc ttgccgaact cacgtgtctt
     7681 gtcgttcagg ttccagtccc agctggtggt cagcaacagg ccctcggtgt tcttcaggcc
     7741 cagactgtgg atgtcggtaa tgaagaccag caagccagcc attttcatgg tcttgctgat
     7801 gccgaattcc ttggccgcct tgatggcatt gatggtgtcg ccaccggcat tggccagccc
     7861 caggatctgc gccttggagt tctgcgcctg cagcaggaag gaggagaaat cggacgcatt
     7921 cagcggatgc ttgacctggc cgagcacctg accgccgttc tccttgacga ccttggcggt
     7981 gtcggcctca agagcggcgc caaatgcata gtctgccgtc aggaagaacc atgtcttccc
     8041 gccctgcttg accacggccc cacccgtgcc ctttgccagc gccgtggtgt cgtaggcgta
     8101 gtgcacggtg tacggggtgc attgatcatt ggtcagtgcc gaggagcccg cgccattgac
     8161 cacgaagaca cgcttcttct cctcggcaat cttggccatg gccagcgccg tgcccgaatt
     8221 ggtgccgcca aacagcatgg tcaggccctg cgtgtctatc cactcgcgcg ccttgctggc
     8281 tgcgatgtcg gccttgttct ggtggtcggc cgaaagcagc tcaatgggct tgcccagtac
     8341 cttgccgccg aagtcgtcga tggccatctg gatcgccagc gccccgttct tgccttccac
     8401 gtcggcgtac aggctggaca tgtcggtgat gaagccgatc ttcaccttgt cctgtgcctg
     8461 cgcctgtgtg gcaaccaggc ccaggccagc ggcgccgagc atggcaacca gggtcttgag
     8521 tttgcggttc atgttcatat cgctctcctt ctctctatgg tcagaaatcg aaaactgttg
     8581 cgccagctca gggttcaaac gcccagcaat tggttgagca cgggcatctt ggcctgcaac
     8641 tcggatgcct cgaagcgttc gacgatgcgg ccatgctcca tgacgtagaa gcgatcggcc
     8701 agtggcgcgg cgaagcggaa gttctgctcc accatgacca ccgtgtagcc cttggcgcgc
     8761 agcgtggtga tcatgcgcgc cagggcctgg acgatgaccg gagccaggcc ttcggagatc
     8821 tcgtcgagca gcagcagctt ggcgccggtg cgcaggatgc gggccacggc cagcatctgc
     8881 tgctccccgc cggacaggcg cgtgccctgg ctgttcttgc gctcggccaa gttggggaac
     8941 atggcgtaga tttcatcgac cgacatgccg gcctggccgg tcttgagcgc gggcggcagc
     9001 agcaggttct cctcacaaga caggctggag aagatgccgc gctcttccgg gcaatagccc
     9061 aggcccaggt gcgctatgcg gtgcgtggcc atgtctatgg tctcggtgcc atagaccttg
     9121 atcgagccct tgcgcgcacc ggtcaggccc atgatggcgc gcagcgtgga ggtgcgtccg
     9181 gcgccgttgc gccccagcag cgtgaccacc tcgccgggct gcaccaccat gtccacgcca
     9241 tgcagcacat gcgattcgcc gtaccaggcc tcaaggccct tgatttccag agcgggagtg
     9301 cctgccatgt cagtgcgccc cctgcagttc gccgtcggtc gtgcccatgt aggcttccat
     9361 cacttgcggg ttgttcgaga cttcttcgta attgccttcg gccagcacgg cgccgcgctg
     9421 cagcaccgtg atccggtcgg cgatcgtgga gatgaccttc atgttgtgct ccaccatcag
     9481 gatggtgcgg ccggccgaca ctttcttgat gagctgcgtg acgcggtcca cgtcctcgtg
     9541 gcccatgccc tgggtgggct cgtcgagcag catcagctcg ggctccatgc ccagtgtggt
     9601 ggcaatctcc agcgcgcgct tttggccata tggcaggttc accgtgacct cgtcggccat
     9661 ctcttccagg cccacctcgg ccagcaactg gacagcgcga tcgttgagct ggttcagcga
     9721 actttggctg cgccagaaat ggaacgaggt gcccagcttg cgctgcaggc ccaggcgcac
     9781 gttctccatc agcgtgaggt gcgggaacac ggccgagatc tggaacgagc ggatgacgcc
     9841 gcggcgcgcg atctgcgccg gctgctcgtt ggtgatgtcg gtgccgttga agacgatgct
     9901 gcccgaggtt ggcaccagga atttggtcag caggttgaag caggtagtct tgcccgcgcc
     9961 gttagggccg atcagcgcgt ggatggagcc gcggcgcacg ctcaggttca cgtcgctcac
    10021 ggccgtgaag cccttgaatt ccttggtcag gtgggatgtt ttcagtatga cgtcgtcgct
    10081 cattgccacc aagcacgccg cgcacggcgg tgcgcgacgg ctttctcctt gtatatgttg
    10141 cggcgcatca tatggggcgc aagctcggaa tttgaacggg agaaatgcct acgtacaaac
    10201 gcctaaaccg acgcaactca ttacacgccc ggccttttcg tgatagttca aaccgccaga
    10261 acaacggccg actcatcgat gaccagcacc acgcgtgagc gtgcccgcag acccgagtcc
    10321 ggcgcggcaa agcccaccat ctgcacgcct gcatccagct gcacagccac ctcgtcgccc
    10381 agctcgccgc gcgacacgcg actggcccgg cctggccatt ggttgccgcc tgccggcacc
    10441 ggcgccttgg tggccgccag cacatgtacc gccgtggcct tgcacagcgc ctgcaccggc
    10501 acacccgcat gcaggcccag cagttccgcg ctctccagcg tgatgcgcgc agtcaccgcc
    10561 aggccggcgg ccgcagggcc cacgccacac aggtgcacgc gcgcgatctg accacaagat
    10621 cgcatgtcct gcacgatgca cggccactga ttgcgcatgc tggtgcgcag cgacagccgc
    10681 gccacggcca ggtctgcgcc gccgccctcc agctgcgcca atgccgacac gcgcgcctgg
    10741 cgcacggcgt cggccgcggc cagcaactgc tcggccgcgg gcgtgagccg cgcgccgcca
    10801 ccacccgcgc cgcccaccac gcgttcgacc agggtcacgc cggccaggtt ggtcagcgtg
    10861 tccagcgcct gccaggcggc cttgtagctg acgcccgcag cgcgcgcggc ctgcgagatg
    10921 gaaccgccct gccctatctg gcgcaggatg tcgatgcgct tgtcgaccat gccatgcccc
    10981 agggcattca ccaaggattc ggattgctgc atgggcggca tcatgccttt gagcgttcag
    11041 gtgctggcta tacttctcgc tattcatatt tggaatagcg aggcgattca tgcacccttt
    11101 gcgctccctg gctctggccg ccgttctgtt gcccacggca catgccggcg aggtttccgt
    11161 cgccgtggcg gccaacttca cggcacccat gcagaagatt gccgcgcaat tcgagcagga
    11221 cacgggccac aaggcccagc tgtcgtttgg cgccaccggc aagttctatg cgcagatcgc
    11281 caacggcgcg ccgtttggcg tgttgctggc agcggacgac accacccccg aaaaaattgg
    11341 caaagaaggc ctgggcgatg gcagcacgcg cttcacctat gccatcggcc agctggtgct
    11401 gtggagcaaa cagcccggct acgtggatgc cgacggcaag gtgctgcaaa agaccgactg
    11461 gcagcatatt gcaatagcca accccaagct ggcgccctac ggcctggcgg ccatgcaaac
    11521 gctgaacagg ctgggcctga cggcacaggt gcagccgcgc atcgtgcagg gcgaaaacat
    11581 tggccagacc taccagttcg ccgcatccgg caacgcgcag ctgggcttcg tggcgctgtc
    11641 gcaggtcatg gagggcggca agctgcgcga aggctcggcc tgggtcgtgc cggccagcat
    11701 gcatgagagc atccgccagg acgccatcgt gctcaagccg ggtcagggca acgcagcggc
    11761 ggccgcgctg atggactacc tcaagggcga caaggcgcgc gccatcatca agtcctacgg
    11821 ttattctttc tgacccgccg accaccaccc gcaaaacctt gccattgctg tcccccgaag
    11881 actggtctgc catccggctc acgctggagc tggccggcat caccaccgta ctgctgctgg
    11941 tgctgtgcac gccgattgcc tggtggctgg cgcacacacc ctcgcgctgg cgcggcccgg
    12001 taggggccgt agtggcgctg ccgctggtgc tgccacccac ggtgatcggg ttctacctgc
    12061 tggtgctgat gggccccagt ggcccggtcg ggcggctcac cgagtcgctg ggctggggcc
    12121 gcctgccctt taccttctgg ggcctggtcg ttggctccat cgtgtattca ctgccgtttg
    12181 ccgtgcagcc gctgcagcgc gcgttcgaat ccatgggccg gcgcccgctg gaagtggccg
    12241 cggccctggg cgcggggccg ctcgatcgtt tcttcaccgt cgccctgccg ctggcgcgcc
    12301 cgggcttcat caccgccgcg gtgctgagct ttgcccacac cgtgggcgag tttggcgtgg
    12361 tgctgatgct gggcggcaac atcccgggca agacacgcgt ggtgtcggtg cagatctacg
    12421 accatgtgga ggccatggag tacgcccagg cacattggct cgcgggcggc atggtggcat
    12481 tcgccttcgt cgtgctgatg gcgctgcatt ggctgcagcc caaggggcgg ctgcaatgac
    12541 cggcatcaac gcgcgtctgc gcctgcaacg ccccgacttc ctgctcgacg tagacctgca
    12601 actgcccagc cagggcgtga cggcgctgtt tgggccatca ggctgcggca agaccacctg
    12661 cctgcgcgcc atcgccgggc tggaacgcac cacgggccgc gtcgaggtca acggccaacc
    12721 gtggcaggac gatgcggccg gcctgtggct gcccacgcac aaacgccagc tgggctatgt
    12781 gttccaggaa gccagcctgt ttgcccatct gaacgtgcgg cagaacatgg aatacggcct
    12841 gcgccgcctg gcgcccgagc gccgacgcgt gtcgctggat caggccgtcg agctgctcgg
    12901 gctgtctgca atgctggcgc gcatgccgca cacgctgtcg ggcggcgagc gccagcgcgt
    12961 ggccattgcc cgagccctcg ccgccagccc acgcctgctg ctcatggacg agcccctggc
    13021 cgcgctggac gcagcgcgca aggccgaggt gctgccatat ctggagcgcc tgcagcgcag
    13081 cctggacatc ccgatgctct acgtcagcca ctcccccgac gaagtcgcgc gcctggcatc
    13141 gcacctggta ctgctgcagg ccggccacgt caaagtccag ggccccacgg ccgagctgat
    13201 gacgcgcctg gatctgcccc tgtcccatgg cgacacggcc gctgcactgg tgcaggcaca
    13261 ggtgctggcg cacgatgcgc acgatcacct gctcagcgtg gccttcagcg gcggccggct
    13321 gcacctgatc gctacccagc aatatgtagc gggcgaagcc gtgcgcctgc gcgtgctggc
    13381 gcgcgatgtc agcctggcac gtacacgcgc caccgacagc agcatcctga acctgctgcc
    13441 ggcacgcatc acgcagctgg ccgaagacag cccgggccag atgctggtgg cgctggacgc
    13501 cgcgggtacg gcgctgctgg cacgcatcac ccggcgctcg tgtcagcaac tggcgctggc
    13561 cgttggggac acagtgtttg cccaggtgaa gggtgtggcg ctggtggatt gatggttcag
    13621 caggtattgg gtttaggctg tgcagataca gcgccaccct caccacacat gaacaccaaa
    13681 cacgcccacc tcacgccgac agagcacatc cccttcgaca ccctgcgcac gacgctggcg
    13741 cacatcttcg aacgccacgg caccagcgcc gccgtcgccg ccgtgctggc cgacaactgt
    13801 gccagcgccg agcgcgacgg cgcgcacagc catggcgtgt ttcgcattcc cggctacctt
    13861 tccacgctgg ccagcggttg ggtggacggc cacgccatac cccgggtgcg ggacatggcg
    13921 ccaggctttc tgtcggtgga tgcgtgcaat ggctttgccc agccggccta catgatggcc
    13981 tacgacgccc tggtggccaa ggcgcgtgcc aacggcatcg cgctgctggc gatccacaac
    14041 tcgcaccatt ttgctgccct gtggcccgac gtcgagccgc tggcgcgcga cggactggtg
    14101 gcgctggccc tggtcaacag catggcctgc gtggtgccca gcggcggcgc caaggcgctg
    14161 tttggcacca accccatcgc cttcgccgcg ccacgcgccg gccatgaccc gctggtattc
    14221 gacctggcca ccagcgccat ggcgcatggc gatgtacaaa tcgccgcgcg cgagggccgg
    14281 ccactgcccg agggctttgg cgtggatcgc catggtcagc caacaaccga cccacgcgcc
    14341 gtgctggacg gcggtgcgct gctgcccttt ggcggcgtgc acagcagcta caagggctcg
    14401 gccctgtcga tgatggtgga gctgctggcc gcggcgctca ccggggggca cttttcgttc
    14461 gaattcgact ggagcggcca ccccggcgcg cacaccccgc acaccggcga actgatcatc
    14521 gccatcgacc cggccaagaa ccaggggcgc gactttgccc agcgctgcga agacctggtg
    14581 cgggccatgg ccggcgccgg ccagcagcgc ctgcctggcg agcggcgcta ccgccagcgg
    14641 gcgcagtcgc tgcgcgaagg cgttgcgcta ccggccgcca cctgggccga attgcagcgc
    14701 ctggcgcagg cgtagttttg acaaaatatg gctctagcgc ccgcccatca agcgctaaca
    14761 gctatcaaaa tcagacgttg atggccgctg ccgacccggc ctgcttgcgc agttcaaact
    14821 tctggatctt gccggtgctg gtcttgggca gctcgccaaa caccacggcg cgcggcacct
    14881 tgaagccggc caggtgctgc ttgcaatgcg ccacgatgtc ctcgggcgtg gtctgggcgc
    14941 cggccttcag ctcgacgaag gcgcagggcg tctcccccca cttcggatcg ggcctggcga
    15001 ccacggcggc ggccagcacg tcggggtggc gatacagcac gtcttccacc tcgatggagg
    15061 agatgttctc gccgcccgag atgatgatgt ccttgctgcg atccttgatc ttgatgtagc
    15121 cgtcggggta ctgcacggcc aggtcgcccg aatggaacca gccaccgcgg aaggcctctt
    15181 cggtcgcctg ggggttcttc aggtagccct tcatggcgat gttgccgcgg aacatgatct
    15241 cgcccatggt ctcgccgtcg tgcggcacgc tctgcatggt ctcggggtcg agcacggcgg
    15301 ccgcgcgctg caggtggtag cgcacgccct ggcgcgcgtt caggcgggca cgctcgccga
    15361 tgtccagatc gccccaggcg tcgtgcttgg cgcacaccgt ggccgggcca tagacctcgg
    15421 tcaggccgta gacatgggtc aggtcgaagc cgagcttttc catgccctcg atcatcgagg
    15481 ccggcggcgc ggcgccggcc accatggcct tgatgcccgc cggcacgccg gccttcatgg
    15541 cggccggggc attcaccagc aggccatgca cgatgggtgc gctgcagtaa tgcgtgacac
    15601 catgcttgcg catggcatca aagatggcct gtgcctcgac gcggcgcagg cagacgttga
    15661 cgccggcacg cgccgccacc gtccacggga agcaccagcc attgcagtgg aacatgggca
    15721 gggtccacag atacacggca tgcttgggca tgtcccactc cagcacgttg ctgatggcgt
    15781 tgatggtggc gccgcggtgg tggtagacca cgcccttggg gttgccggtg gtgcctgacg
    15841 tgtagttgag ggcgatggca tcccactcgt cgcccggcag ctcccaggcg aactgggggt
    15901 cgcccgcggc cagaaactcc tcgtaggtct gctggcccac gcgcagctcg gccgcgccat
    15961 agacctcgtc ctccacgtcg atcagcagca acggcgcctt ggactggcgc agcgtaagcg
    16021 ccttggcaat gacggtggcg aactcgggat cgacgatcac cgccttggcc tcgccgtgat
    16081 ccagcatgaa ggcgatggtc tcggggtcca ggcgcgtgtt cagcgcgttg agcacggcgc
    16141 cggccatggg cacaccgaaa tgcgcctcca ccatgggcgg agtgttgggc agcatcaccg
    16201 ccacggtgtc gttcttggct atgccggcgc gctgcaggct gctggccagg cgacggcagc
    16261 gcgcgtaggt ctgggcccag gtctggcgca gctcgccatg gacgatggcc aggcgctctg
    16321 ggtaaacctc ggccgtgcgc tcgatgaagg acaggggcga cagcggggcg tggttggccg
    16381 cgttgcgtga caggttctgg tcgtacatgt ggttggcggt catggaaact ccgaagcaaa
    16441 aattcagtac ccccgattac aacaaacccg cctgacgccg aactgagcgc gcgggcaaga
    16501 ggctgagaga aaatagcccc gtgtccatcc cccagtcgtt catccaggag ttgcttgcgc
    16561 gcgtcgacgt ggtcgatatc gtcggccgct atgtgcagct gaaaaaaagc ggcgccaact
    16621 tcatggggct gtgccctttt catggggaaa agtcgccctc gttcagcgtc agcccgtcca
    16681 aacagttcta ccactgcttt ggctgcggca agaacggcaa cgccattggt ttcctgatgg
    16741 accatgccgg catgggcttt gtcgaagcgg tgcaggatct ggcccagcag accggtatgc
    16801 aggtgccgca ggacgaccag tcaccccagg atcgcgagcg cgccgcagcc cagcggcaga
    16861 aacaggccac cctgaccgac gtgctggaga aggccggcga ggcctggcgc aagcatctgc
    16921 gcgacagtcc gcgcgccatc cagtacttca agggccgggg tgtatcgggc cagatcgcca
    16981 gtcgctatgg cctgggttac gcgcccgaag gctggcgcgg cctggccagc gtgttcccgg
    17041 aatacgacag cccgttgctc gaagaaagcg gcctggtgat cgtgaatgcc gaggacggca
    17101 aacgctacga ccgctttcgc gaccgggtga tgttccccat ccgtaacgtg aagggcgaat
    17161 gcataggctt tggcgggcgc gtgctgggcg acgacaagcc caagtacctg aactcgcccg
    17221 agacaccggt attccacaag gggcgcgagc tctacggcct attcgaggcg cgtcaggcga
    17281 tccgcgagat gggctacgcc ctggtcaccg agggctacat ggacgtggtg gccctggccc
    17341 agctggggtt tgccaacgcc gtggccacgc tgggcacggc ctgcacgccg gatcatgtac
    17401 acaaactgtt tcgtttcacc gagcaggtgg tgttcagctt tgatggcgac gcggccgggc
    17461 ggcgcgcggc gcgcaaggcg ctcgatggcg ccctgcccta tgccagcgac acgcgcagcg
    17521 tgaagtttct gtttctgccc tccgaacatg accccgacag cttcatccgc gcccacggcc
    17581 ccgaggcctt cgcgcgccat gtgggcgagg ccatgccgct gtcgcgcttc ctgctggaag
    17641 ccgcgcgcga aggctgcgac ttagggcagg ccgagggccg tgcccatatg ctgagcaacg
    17701 cgcgaccact atggtctgcc ctgcccgatg gcgcactcag gcggcaactg ctggccgagt
    17761 tgggcgagct cggtcagctg ccagccacag agctggccag cctgtgggca caagacggcg
    17821 cacgccaccc cggcccagcc ggcgcggcgc acaacgctcc tccgttcggc gacgaaccac
    17881 cctggggcca ggagccgcca gggtcatggc ctggcgacac cgcacagacc acccgaccct
    17941 ggcgcaagag cggcgactgg aagggcaagg gcggccgctg gcgccgcgag gaacagcgcc
    18001 ccgcccaacc gccgctgccg cgcacgccgg tggccggccg cgccgaccac gcagcgcggc
    18061 tgctgctgtc gcacatgcag tttctcgaag aactttcgca cgaagaccat ggcctgctgc
    18121 gcgagcagcc cgcgccccat ggcgcgctgt tcgtgtggct ggagggccag ctgcacgagc
    18181 atggccccca gtcctgggcc gttttgcgcg aggctctggc cggccatgac agccaggccc
    18241 tggccgagcg cctgatgagc ggcagccacg cccagaccga gggcgacgaa gccgagctgc
    18301 gcgccgaact gcgcggcctg ctgaaccgca tgctcatcga tgcgctgaaa caggagcaaa
    18361 acgcactcat cgccgccgcg gcccaggatc ccacggcact gcagcgatat cgccagctgc
    18421 aggagcgccg ccgcatcctg gaaagccatg ggcaaccggg ctcttgaaaa aatccaagtc
    18481 ggtataatgt aaggtttgtc ggcaagagcg acagcagcac ctccggatca ggcaaccgcg
    18541 acaacagcgc gcaaccgccc accccaccgc aaggactgca tcccaaatgt tcgcccagac
    18601 atcttgcctc tgagggcctg cccctcgaac cgcgcatgcc ccggcctgcg ccgtgcctga
    18661 ccgcgccgat accgctggcg cttgtgtttt ctttgtgtgt ccgtttttga ccctgaggtt
    18721 ggttccatgc ccgttcaaaa gtccgcgaag tcgcccaagt ccgccaccgg caccgccgca
    18781 caggccgctt ccgaagctac aaccaccgtg aaaaccgccg ccaaggcgcc cgcgaaagcc
    18841 gccaagtcca aggcagccgc tgcgcccgag gcgacgacga aagtcaagac cgtgccaacg
    18901 cccaaatcct cagccgagct ggaaaaagcc gccgacgaac tgttgaagaa aaaacccgcg
    18961 cgcgccgcca aggctgcccc agccgaagac gcccaagagg cgcccaagaa aaaacccggc
    19021 cgcaaaccca aggccgccga cgacgaggca acggcgccca aggcggccgc caagaaggcc
    19081 cccgccaagc gcggccgcaa gcccaagggc aaggaagaag gcctggagga cgatgccgac
    19141 ctgtccgaca tcgaagccga cctggagggt gagcccgaac ccgaggtttc caccaccgag
    19201 gccgcgcccg ccgagaaggt caagcccctg cgcatgaaga tcagcaaggc caaggaacgc
    19261 gccttgatga aggagttcgg cctggacgag acggtcctgt ccgaagaaga catggccaag
    19321 cgccgcgcgc agctcaagaa gctgatcacc ctgggcaaga cgcgcggcta cctgacgcag
    19381 gtcgagatct ccgaccacct gccggacaag ctggtcgacg ccgagacgct ggaagtcgtg
    19441 atctccatgc tcagcgacat gggcgtggcc gtgtacgagc agacgcccga cgcggaaacc
    19501 ctgttccaga acaacgtcac ccccaccgcc accacggtcg aggaagccga ggaagaggcc
    19561 gaggccgccc tgtccaccgt ggacagcgaa ttcggccgca ccaccgaccc ggtgcgcatg
    19621 tacatgcgcg agatgggcac ggtggagcta ctcacgcgtg agggcgagat cgagatcgcc
    19681 aagcgcatcg agggcggcct gatggacatg atggaggcca tcagcgcctc gcccgcgacc
    19741 atcgccgaga tcctcaacat gggcgaggaa atccgcgagg gcaaggtcat catcaacacc
    19801 gtggtcgatg gcttctccaa ccccgaggag gccgacgact acgtggccga ggaagacttc
    19861 gacgagtacg acgaagagga cgacgacgac ggcaagggcg gctccaaggc catgacgcgc
    19921 ctgctggaaa agctcaagaa cgacgcgctg gagcgctttg acgagctgcg cgcgctgttc
    19981 gagaagatgc acaagatcta cgacaaggag ggctacggca cgccggcgta catgaaggtg
    20041 cagcacgaga tctcggccaa gctgatgacc atacgcttca cggccaagac catcgagaag
    20101 ctgtgcgaca tggtgcgtgc ccaggtggac gatgtgcgca agaaggagcg tgagctgcgc
    20161 cgcatcatcg tggacaagtg cggcatgccg caggagacct tcatcaagga gttcccgccc
    20221 aacctgctcg accgcacctg ggtcgagaag caggccgccg cggccaagcc ctggagcacc
    20281 gtgatgcagc gcaacatccc gccgatccag gagctgcagc agcacctgat cgacctgcag
    20341 tcgcgcgtgg tcgtgccgct gggcgagctc aaggccatca acaagcgcat gaaccagggc
    20401 gaatcgaatt cgcgcgacgc caagaaggag atgatcgagg ccaacctgcg cctggtgatc
    20461 tcgattgcca agaagtacac caaccgcggc ctgcagttcc tggatctgat ccaggagggc
    20521 aacatcggcc tgatgaaggc cgtggacaag ttcgaatacc gccgcggcta caagttctcg
    20581 acctacgcca cctggtggat tcgccaggcc atcacccgct cgatcgccga ccaggcgcgc
    20641 accatccgca tcccggtgca catgatcgag accatcaaca agatgaaccg catctcgcgc
    20701 cagcatctgc aggagttcgg cttcgagccc gacgccagca tcctggccga gaagatggag
    20761 atccccgagg acaagatccg caagatcatg aagatcgcca aggagccgat ctcgatggaa
    20821 acgcccatcg gcgacgacga cgacagccac ctgggcgact tcatcgagga cagcaacaac
    20881 accgcgccgg tggatgccgc catgcaggcc ggcctgcgcg acgtggtcaa ggacatcctg
    20941 gacggcctga caccgcgcga ggccaaggtg ctgcgcatgc gctttggcat cgagatgacc
    21001 agcgaccaca cgctggaaga agtgggcaag cagttcgacg tgacgcgcga gcgcatccgc
    21061 cagatcgagg ccaaggcgct gcgcaagttg aagcacccca gccgcagcga caagctgcgc
    21121 agcttcatcg attcgctgta accgcgctac cgccagccag caagccccgc caccacccgg
    21181 tgccggggct tttttgcgcc ctcattgcgt cgcaccaccg gcggtggaca atgcctgcgc
    21241 accccccctt gccagtcccg ccatgaccac cacccccgat acctctgccc cacgccttgt
    21301 atttgccgtc atgggcgcgg gctcggtggg ctgctacttt ggcgcgctgc tggcgcgcgc
    21361 cgggcacacc gtcaccctca taggccgcca ggcccatgtg caggccatta ccgcccatgg
    21421 tctgcggctg cagagcgcaa cgcttgatga gcatgtgcgc ctgagcgcca gcaccgaagc
    21481 caaggcggta gccggcgccg acgtggtgct gctgtgcgtc aaatccaccg acaccgagac
    21541 cgccgcgcgc cagatccagc cgcatctggc gcccggcgcc ctggtgctga cgctgcagaa
    21601 cggcgtggac aacgacgagc gcgtgcgcgc catgctcggc cccgctcagc ccgtggccgc
    21661 cgccgtggtg tatgtggcca cggccatggc cgggccgggc catgtgcggc atttcgggcg
    21721 cggcgatctg ctgatagccc cctcgcccgt cagcgagcgc ctggcgcttg aactggccgc
    21781 ggctggcatt cccacccagg tgtccggcaa cgtgcgcggc gcgctgtggg ccaagctggt
    21841 catcaactgt gcctacaacg cgctgtcggc gatcacccag cagccctatg gctggctggt
    21901 gcaacaggat ggcgccagcg cggtgattgc cgatctggtg gccgaatgcc aggccgtggc
    21961 gcaggccgac ggcgtgcgca tggacggtga cgtgcatgcc gccgtgcgcg gcattgcgca
    22021 aagcatgccg ggccagctgt catccaccgc ccaggatctg gcacgcggcc gacccagcga
    22081 gatcgagcat ttgaacggct atgtggtgcg ccgcggcgca gcgctgggcc tggccacccc
    22141 ggtcaaccgc gccctgctgg tactggtgcg catgctgcag gcaaaatcca tctaaaatga
    22201 tagctgttag cgcttgactg tagcgcctca cagcccaaag acattcaaaa tcaaaccacc
    22261 gccttggcct ccagcagtgc ctggatctgc ccggcgtcat agcccagccc ctgcagcagt
    22321 ggctcggtgt gctggcccag gcgcggcggg tcgctgcgca cggccagtcg ccggccgtcc
    22381 atgcgcagcg gcagcagcgt ggtgcgcgcc acctgccccg cacgttcacc gtcgggtagc
    22441 gtgatctcgg ccaggccccc ggtggcggtc aggtgcggat cgtcgaacag ctgctcgggg
    22501 cgtgcgatgg gcgcgaacgg caggccgtgg cgctcgaaca ccgccgccag ctcctcggcc
    22561 cggtgctgcc ccagacgccg gcgcagttct gggaccagcg tggggcgctg gcgcacgcgg
    22621 tcgttgttgc tggcgtggcg cgggtcggcc ttgaggtcgg cgtagcccat ggcgtcgcaa
    22681 aaaacttgcc actgcgcgtc gctcaccacc gccagaaaga tctgcccgcc gtccttgaca
    22741 tcgaacacgt cgtacacggc ccaggatgag atgcgctcgg gcatgggtgc ggcgggctgg
    22801 ccggtgacgg cgtactgcat catgtgctgg cccaccagga agacgttgtt ctcgaaaagg
    22861 gccgaatcca cctcctggcc cagtccagtc tgctggcgct gcatcagcgc cgccatggca
    22921 cccatggcgc caaacatgcc acccatgatg tcgttgacgc tgctgccggc gcgcaacggg
    22981 tcgcccgagc ggccggtcat gtaggccagc ccgcccatca tctgcaccac ctcgtccagc
    23041 gccgtgcggt gctcgtacgg cccaggcaga aaaccggtgt ggttcacgta aaccatacgc
    23101 tcgttcaggc gcgagagcgc ggcgtagtcc aggccgtact tggccatcac gcccggc
//
LOCUS       DAOMTQ010000030        22543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_257_length_22543_cov_25.887140, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000030 DAOMTQ010000000
VERSION     DAOMTQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 22543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22543
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_22543_cov_25.887140"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK518_04815"
     CDS             <1..354
                     /locus_tag="PK518_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRM48266.1"
                     /translation="AVAPAPVVPAPAPAPAPAPAVQAAAEPAAGMPRVQAFTLPVQSL
                     MDVAQSSGLQWINSDADKVAAVQAAIAAEPKPVHIPRERPPAVVLDEGPLVLVETRRD
                     LRDLQLPFEKEGAAV"
     gene            501..1277
                     /gene="yaaA"
                     /locus_tag="PK518_04820"
     CDS             501..1277
                     /gene="yaaA"
                     /locus_tag="PK518_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRM48267.1"
                     /translation="MLFLLSPAKSLDYDTPLPAGLPHTLPAFVPQAQQLIAVLRDKSP
                     QEIASLMSISDPLAALNVARYAAWRPRFTADNARQAVMAFNGDVYEGLQAPTLSPDEL
                     NWAQQHLAILSGLYGVLRPLDWMQPYRLEMGTRLATSAGSNLYQFWGQRIAEHLNQRL
                     AQDVTPVVVNLASQEYFKSVDLKTLKARVIECVFEDYKNGQYKIISFYAKRARGLMAR
                     YAIQQRVTTPHQLEGFDLEGYAWAPGASTPERLVFRRQAT"
     gene            1293..2147
                     /locus_tag="PK518_04825"
     CDS             1293..2147
                     /locus_tag="PK518_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRM48268.1"
                     /translation="MSLSQTITPELRQWIIDQASAGVAAPALIEGMRASGWNEDVAIA
                     AVELVLRAHLDKVALDKGLPPAAPVPDPALAESPLHIDVGDRQVEVLMAMANPRVVLF
                     GNLLSPEECQAIIDAAGSRMARSLTVQTATGGQEVNQARTSDGMFFERGENAVVARLE
                     QRIARLVNWPVQNGEGLQVLHYRPGAEYKPHYDYFDPQEAGTPSILQRGGQRVATLVI
                     YLNSPAKGGGTTFPDVPLEIAPRQGNAVFFSYERPHPSTRTLHGGAPVTEGEKWIATK
                     WLREREFK"
     gene            2207..3052
                     /gene="queF"
                     /locus_tag="PK518_04830"
     CDS             2207..3052
                     /gene="queF"
                     /locus_tag="PK518_04830"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913989.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HRM48269.1"
                     /translation="MSTPEQSQLGKASAYADQYDAALLFPIPRQPKRAEIGIAGAPSF
                     FGADLWTAYELSWLNLRGKPQVAIAHITVPCETPNIVESKSFKLYLNSFNNTRFASLD
                     EVRARIRQDVSEAAWRGAAHAGTVGVRLVTPDQFDAEQVHELDGLLLDRLDIECTQYQ
                     PAPELLKANHDEAPVTETLVSHLLKSNCLVTGQPDWGSVQIAYSGAQIDQEGLLQYIV
                     SFRNHNEFHEQCAERIFMDIWQRCRPIKLAVYARYTRRGGLDINPLRTSAPMPLPANR
                     RTARQ"
     gene            3108..3923
                     /locus_tag="PK518_04835"
     CDS             3108..3923
                     /locus_tag="PK518_04835"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRM48270.1"
                     /translation="MNTPPRMPTLYIPHGAGPCFFMDWNPADAWDKTAAYLRSIASSL
                     PTRPQAIVLVTAHWLGAQPCMSSAARPGMLFDYYGFPPHTYELSYPAPGAPALAGRVS
                     GLLAGAGFDPVQDSERGYDHGSFIPLLLAFPDADIPVLQLSLLRSLDAAEHLRMGRAL
                     RPLRDEGVLIIGSGMSYHNMRGYGDPRSGPAAAEFDRWLSETVALPTAERDARLANWA
                     TASGPAGRLSHPPRAEEHLLPLHVAAGAAGEGRGAKVFGDRVMGVDISAFRFD"
     gene            4136..4351
                     /locus_tag="PK518_04840"
     CDS             4136..4351
                     /locus_tag="PK518_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48271.1"
                     /translation="MPAALDAVAVRHGMRPGEVTPALVRARPAVVAPITGATGLEPLG
                     GLIRATRLRLSARDMATFDAAGAAEAP"
     gene            4357..4854
                     /locus_tag="PK518_04845"
     CDS             4357..4854
                     /locus_tag="PK518_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48272.1"
                     /translation="MTGRDLVTLQILEHVALIQSRPGGDSSIEPASVAAPLGRAVLAA
                     TLRHDPPTPLELEHAIEQIEDALMPLRAQVAGARLVFANAALHALARAGAGANGSAPG
                     ADITLDMVDVETLFTRLAARANGRPATQDCLPTDPASSAALLVAREVLHHWGLGALTV
                     RDAAA"
     gene            complement(4878..6029)
                     /locus_tag="PK518_04850"
     CDS             complement(4878..6029)
                     /locus_tag="PK518_04850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRM48273.1"
                     /translation="MDAFYASVELLRYPQLAGLPVVIGGGRRQQDDALAAQYGERLAD
                     IPVAAFPRLADYVGRGVITTATYAARQFGVGSAMGLMKAARACPQAILLPVDFAEYRR
                     YSRRFKQVITDIAPVMEDRGVDEVYIDFTQVPGGQREGGRVLARLIQKSIFEATGLTC
                     SVGVAPNKLIAKMASEFNKPNGIATVQPDDLQTLVWPLACRKINGVGPKADEKLQSYG
                     IHTIGELAARPRDWLVAHFGRSTGAWLHDAAWGRDDRPVVLESEPVSMSRETTFDRDL
                     HAVQDRALLGQIFTELCQQVAADLQRKGYAGRTIGIKLRYDDFRVATRDHTIVLPTQD
                     ATVIRREAGLCLKRVPLDKRLRLLGVRVGKLLPQSEVGRQAQGEGEALF"
     gene            complement(6098..7030)
                     /locus_tag="PK518_04855"
     CDS             complement(6098..7030)
                     /locus_tag="PK518_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM48274.1"
                     /translation="MSLSLEAPAWLWVPVVLFATLAQTARNTAQRTLTAELGTLPATL
                     VRFLYGLPFAGLWLALLYCWPDQPPALPHFSAAYLGWIALGAFFQIAGTAFLLLAMAE
                     RNFAVAVTLSKTEVLQVALFATVLLHELPRPLALLAMVVATTGVFLLSLPPRGQLLSL
                     SAWLSRPALYGLGCGASFAIASVGFRGGALALGAETPWLSGAWGVVIAQLLQSLGLGA
                     WVALRSEKGLAPLWPAWRLSLVAGSMGAAASLAWFTAYAMQGAAEVRTLGMVEVVFSY
                     LVSRRLLSESFTRPEKLGMALMLVGLVLISLQGL"
     gene            complement(7271..8443)
                     /locus_tag="PK518_04860"
     CDS             complement(7271..8443)
                     /locus_tag="PK518_04860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRM48275.1"
                     /translation="MADLSKMTCIEDLRQVAECRVPRMFYDYADSGSWTEGTYRANES
                     DFHAIQFRQRVAVNMEGRSTATTMVGQQTSMPVCIAPVGLTGMQHADGEIHAARAAEK
                     FGIPFTLSTMSICSIEDIAEHTSAPFWFQLYMMRDRDAMARMIQRAKDAKCSALVLTL
                     DLQVIGQRHKDIKNGLSAPPRPTLKNIINLMTKPRWCLGMAGTRRHTFRNLVGHVKGV
                     SDMNSLAAWTNEQFDPRLSWEDVRWVKQQWGGKLILKGIMEVEDALLAANNGADAIVV
                     SNHGGRQLDGAPSSIAALPAIVDAVGDKLEVWMDGGIRSGQDVLRAWALGAKGTMIGR
                     AMVYGLGAFGEAGVTKALQIIHKELDVSMAFCGHTNLQNVDRSILVPGSYPVAPRG"
     gene            8557..9261
                     /locus_tag="PK518_04865"
     CDS             8557..9261
                     /locus_tag="PK518_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRM48276.1"
                     /translation="MITLYTAATPNGHKVSIALEELGLPYRLQVLDLSAGEQKRPEFL
                     AINPNGRIPAIVDHDAGDFAVFESGAILVYLAEKTGRLMPQDAPGRSRVLQWLMFQMG
                     GVGPMMGQANVFYRYFPEKIQPAIDRYQAEGRRLFEVLDGHLAQHEWLAGDYSIADIA
                     NWAWVRTHRWSGISIDGFDHLRRWRDAIRARPAVQRGLLQPPAKMDLERDGEAGAQAF
                     AAQARTMVITGQQEKP"
     gene            9258..9881
                     /locus_tag="PK518_04870"
     CDS             9258..9881
                     /locus_tag="PK518_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRM48277.1"
                     /translation="MKLYTAQRAPNPRRVLMFIHEKAITGIELVPVDLNAGEHKGAAY
                     RALNPVATVPTLELPDGRCLSETRAICSYLEGLQPEPNLMGRDFAERAFIEMHDRRVE
                     WYWLLPIANCVRHTHPGLATLERPQFADFGRSQGEKVRERARWLDEQLAEQPWMAGER
                     FTIADITAFCALEFARGLMKFNAGAEGFARLQAWRERMAERESARVF"
     gene            complement(10044..10505)
                     /locus_tag="PK518_04875"
     CDS             complement(10044..10505)
                     /locus_tag="PK518_04875"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRM48278.1"
                     /translation="MPSHTELLRLAVDLAHANRQRGGRPFGAVLARDGEVIATGINDI
                     VHTHDPSAHAEMQALRAGTRRQASPSLAGCTLYASGHPCPMCLAALLMAGCGQVFYAF
                     DNQDATPYGLSNEGWYRCLRLPLVPPPLPLSRLDTGISAAQLYGDTPWPQA"
     gene            complement(10553..12754)
                     /gene="pbpC"
                     /locus_tag="PK518_04880"
     CDS             complement(10553..12754)
                     /gene="pbpC"
                     /locus_tag="PK518_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRM48279.1"
                     /translation="MRLGAWAAGLALAAGPALALPSFDEVRADFRSSETLVLSREGEV
                     IQRLRTDATVRRGQWLGLADVSPALQRALVLSEDRRFYEHSGVDWAAVSAAAWANLWH
                     ERTRGASTLTMQLAGLLDGDWRQGPGGRTVTQKMGQAVAAQLLERRWRKDQILEAYLN
                     LVPFRGELVGIDALARTLFGKAAHGLDERESAVAAALVRAPNARPALVARRACGVLRD
                     MQQGQADCEALDLFTTAALQRRAYDASAGIAPHFARQWLRGGHGAGQRSSLSAPLQRM
                     AQQTLQRHLRELAGRNVQDAALVVLDNASGEVLAWVGSSGPLSQAAEVDGVLALRQPG
                     STLKPFLYAQAIAEKRLTAASLLHDSPAQIATAGGLYIPQNYDRQFKGWVSVRQALGS
                     SLNVPAVRTLVMVSPDAFFAQLRRLGLALRESGGYYGYSLALGSGEVRLLELTNAYRA
                     LANGGRLSPVAYAPGARPVLTQALDAGAAFIVGDMLADSNARVPSFGTDSVLNTRFWS
                     AVKTGTSKDMRDNWAMGWSQRYTVGVWVGNAGGSPMHDVSGSSGAAPIWADVMAWLHR
                     AAPSHAPRPPAGVLRQPVRFDGGADRLESPREEWFVRGTQQAVFAIDIEAPDAYIASA
                     SGKKHMNFGTPARIIAPAPGSIIALDPDIPPAHQRLQLQAQGGTALRWRIDGRPVGQG
                     AALAWLPWPGRHRVELLDAQGQMQDSVQIEVRGAGVKPGPGRPGGAGGAMP"
     gene            complement(12849..13196)
                     /locus_tag="PK518_04885"
     CDS             complement(12849..13196)
                     /locus_tag="PK518_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3088 domain-containing protein"
                     /protein_id="HRM48280.1"
                     /translation="MPKDQLFLLQPGFFKESEGPFYCGDSVAVEGLLSFFPQLRNEVD
                     VHYIGAPRPRAAIVALIGADNQSAPVLVLGHGRVVSDAGVETRTHNGVRFIDAPDQIR
                     RYLSAQYGVAHAG"
     gene            13331..14296
                     /gene="ftrA"
                     /locus_tag="PK518_04890"
     CDS             13331..14296
                     /gene="ftrA"
                     /locus_tag="PK518_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HRM48281.1"
                     /translation="MDTPHTAPGLVAVVVYNGLSLFEYGCAVEVFGLPRPELGDRWYR
                     FAACAAEPGVLRGAGGIQIQPDQGLELLAQARTIIVPGWRGIDAPVPEPLSQALRQAH
                     ARGARVLSICTGAFVLAAAGLLDGRRATTHWRHAQTLAARYPRVQVQADVLYVDAGDI
                     LTSAGSAAGIDLCLHLIRRDLGARMANQVARRLVMPPHREGGQAQYIEEPVPRRSNAS
                     LAPLLDAMRASLGQDWPIAKMAAHSATSARSFQRHFVGAMGMPPGEWLLAQRLAAART
                     MLEETDASVDEIALQVGFGDAATLRGHFRNRLGTSPGGYRKRFRA"
     gene            complement(14454..15551)
                     /locus_tag="PK518_04895"
     CDS             complement(14454..15551)
                     /locus_tag="PK518_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM48282.1"
                     /translation="MSLIQCLPSGPLDIVGDIHGEIAALRQLLTHLGYDEQGHHPQGR
                     RLVFVGDFCDRGPDSPAVLRLVLSMIAAGTALAVLGNHEINLLREDAKDGSGWFFDSR
                     LASDEPKYAPFARLSKADAATLLQQLNQMPIALEREDLRVVHAAWLSPQIAMARSLPV
                     GDVRHAYDRYEDQAAEQARSSQVLQRMQTERQRWPHSLEDGAQRPPFLPAHSERELGK
                     AMVNPLKVLTTGVERECRTPFYAGGKWRFVQRVAWWSEYTEAPAVVVGHYWRRPLAGD
                     AAAHGPQAENLFGHTGPLAWLGARGNVFCVDYSVGARWAARRNGEDPARRFKLAALRW
                     PERTLVFDDGVQLATEGFGAPGKAQAAARPS"
     gene            complement(15758..16027)
                     /locus_tag="PK518_04900"
     CDS             complement(15758..16027)
                     /locus_tag="PK518_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRM48283.1"
                     /translation="MNGNKHAMPTAWVDPDDAPELTDAFFEQADEYVGEKLVRRGRPK
                     AAVTKEAVKLRLDADLLAALRATGDGWQTRINDMLRASMHLAGRV"
     gene            complement(15987..16259)
                     /locus_tag="PK518_04905"
     CDS             complement(15987..16259)
                     /locus_tag="PK518_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRM48284.1"
                     /translation="MLIEFDPDKRDKALAERGLDFSRAAEVFEARHFTAEDERAGYSE
                     PRYITVGQLDGRMVVMVWTPRGEARRIISMRKANEREQARYAHRLG"
     gene            complement(16314..22244)
                     /locus_tag="PK518_04910"
     CDS             complement(16314..22244)
                     /locus_tag="PK518_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRM48285.1"
                     /translation="MNGGVRGAMAALLLLATAAAQALTVTSLSPQGEVAQVRQVVAKF
                     DAPAVRLGDPRAPAPFTLACSDPDAARGQGRWSGEREWVYEFAQDLPPGVRCTVSAKP
                     GFKSASGALLSSAKSYQFNSGGPFVQSIRPGTHEAIDEEQFFVLQLNGPASVASLQEH
                     MWCLADGVGERVPVRLVEGAQRTELLRALHLDKAAAKEPLRYATLTCGRRLTAGSKMQ
                     LVYGRGVATPSGVANAVEKRFAYQVRQPFTAEFACERENAQSDCLPIRPLRLAFNAPV
                     TRKQAQGIRLKGGGQTLMPTMDGAQEGDALVDAVAFAAPLTAQTRFTLELPADFQDAS
                     GRRLGNVASFPLKVATGPMPPLAKFAAAPFGVVERYAEGPDGPALLPVTLRNIEAQLD
                     AKTLQPGAGQGGKVSTLRADSDADIIAWLRKLQRYDDHYVDRKQALRDVKGPLPKVLD
                     ERDGDQVQARMLSLLAGQAGVRTLDIPQPTKGDPRPFEVVGIPLPPGFSVVEIASPLL
                     GQSLLDGRHGSPRSMVVRTSALVTNLGVHFKLGRENAVAWVTTLDKGQPVAGARVRVS
                     SCDGRELASASTGADGVARIAGLASEPPACGGEGEWRNAYFVSARAGDDMAFTWSDWQ
                     RGIEPWRFNLPTSSAPRPDEVAHTIFDRTLLRAGETLSMKHVLRTQTGAGFALPRQRP
                     GTLVITHVGSGQQYQQPLKWRNTATGGLSAESEFAVPAAARLGQYQVELRASEEGGGQ
                     QSGSFRVEEFRLPVFEGRVTPVAGKPLVRARSVPVDVQVSYVSGGPAARLPVRVSAMV
                     APKPLQFADFEAFSFAPPRKRDAVNNSDEEEPAASQNQRVVADKRPLMLDANGTGRLD
                     IDDIARQAQAQDLVLEASYADPNGELQTLRSTSTLWPAAVVAGVKTEGWVSSGSKVRL
                     QALALSLDGKAQAGVPLAVQAIARSTTSSRKRMVGGFYSYDNRVETKDLGTLCTGKSD
                     ARGLLLCDARLEEAGEVELVVTARDKDGNEAQAASSVWVTRRGELWFGGEDHDRIDLL
                     PEKKSYQPGETARLQLRMPFRQATALVSVEREGVIETRVVQLSGQDPTLQLKIEEGWA
                     PNVYVSVLALRGRLHEVPWYSLFTWGYKAPRAWWSAFWYEGREYAAPTAMVDLSRPAY
                     RLGVAELRVGHEAHRIDVKVKADKDSYPVRGKAQVTIQGTLPNGKPAAGAEVALAAVD
                     QALLELMPNTSWNLLEAMLQRRAWGVATSTAQMEIIGRRHYGKKAVPAGGGGGRAPTR
                     ELLDTLLLWQPAIKLDARGQAKVTVPLNDALTTFKIVAVADAGTGLFGTGSASIRSAQ
                     DLQIISGLPPLVRGGDAFRAQITLRNTTARAMRVEVAPRATLLSLDRQTVAIPPGEAR
                     ELAWNVTAPEQLGQMRAQAILWEIEARDTLGGARDALKASQRLIPAVPLAVQQARLVQ
                     VDGGYQVEVTPPADAIAGRGGLKLSLTPKLADGLPGVRDWWANYPFSCLEQKASKAMG
                     LADAGLWKTVLAQLPSYLDGDGLAYYFPPRDGAADRGSDTLTAYLLASSHEAAGLRPE
                     FALPPELREAMERGLIAFVEGRIERDFWSPRKDLDMRKLAAIEALSRYAKAQPRMLTS
                     LTIAPNQWPTHALIDWINILKRMDGVPQRAERLRDAMQILRARLSYQGSRLSFSTEQG
                     DTWWWLMQGGDVNGARLLLTVLDDPAWKDDLGRLASGFIARQQGGAWHTTTANLWGGL
                     ALEKFSASLEATPVAGSTRASLGGNTASVDWGKVARITAADSGGAAHQTTWFGAPAAP
                     GGLKNNAMFLPWGKGAESLTVTHQGTGKPWLTLQSVAAVERKAPFSAGFAIKRTVTPV
                     EQADKTRPAGTYSRGDVLRVTLEVTASADMSWVAITDPIPAGATILGSGLGRDSEIAT
                     QDEKRQGPGWPAFEERSFESLRAYYEYLPKGTVTMQYTLRLNNVGDFALPASRVEALY
                     APEMFGEAPNTRVRVAPE"
     gene            complement(22264..>22543)
                     /locus_tag="PK518_04915"
     CDS             complement(22264..>22543)
                     /locus_tag="PK518_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRM48286.1"
                     /translation="LPDVPTFAELGLKGFEDTPYYGFYAPAGTPQAFIQRFSDALAQV
                     VAIPEVRDQLTAMGLTVGTMTPEQLTTRERAYTAVWRRIILDSGFRPQ"
BASE COUNT         3549 a         8034 c         7527 g         3433 t
ORIGIN      
        1 gccgtcgccc cggcacccgt ggtgccagca cccgcgccgg caccggcacc cgcgccggcc
       61 gtccaggccg cggcagagcc ggccgccggc atgccacgcg tgcaggcctt cacgctgccc
      121 gtgcagtcgt tgatggatgt ggcccagtcc tcgggcctgc agtggatcaa ctccgacgcc
      181 gacaaggtgg ccgccgtcca ggccgccatc gccgccgagc ccaagcccgt gcacatcccg
      241 cgcgagcgtc cgcccgccgt ggtgctggac gaaggcccgc tggtgctggt ggaaacccgc
      301 cgcgacctgc gtgatctgca actgccgttc gagaaggaag gcgccgccgt ctaaagtaag
      361 gtcacgcctg acagcaccgg gggccttggc ccccggtgct gttttatcac tatgaaaaat
      421 ctagctgttt gcgcttgcca gtcaagggtt caaggccaat ttgatgcaat tacgaacagc
      481 taggcgcacc ggagccccgc atgctgtttc tgctctcccc cgccaagtcg ctggactacg
      541 acacgccgct gcccgccggt ctgccgcaca cgctgccggc ctttgtgccc caggcccagc
      601 agctgatcgc cgtgctgcgc gacaagtcgc cgcaggagat tgcctcgctg atgtccatca
      661 gcgacccgct ggcggcgctg aatgtggcgc gctacgccgc ctggcggccg cgcttcacgg
      721 ccgacaacgc gcgccaggcc gtgatggcct tcaacggcga tgtgtacgag ggcctgcagg
      781 cccccaccct gtcgccggac gagctgaact gggcgcagca gcatctggcc atcctctccg
      841 gcctgtacgg cgtgctgcgc ccgctggact ggatgcagcc ctaccgcctg gagatgggca
      901 cgcgcctggc caccagcgcc ggctccaacc tctaccagtt ctggggccag cgcatcgccg
      961 agcacctgaa ccagcgcctg gcccaggacg tgacgccggt ggtggtgaac ctggcctcgc
     1021 aggagtattt caagtctgtc gatctgaaaa cgctcaaggc acgcgtcatc gaatgcgtgt
     1081 ttgaagacta caaaaatggg cagtacaaga tcatcagctt ctacgccaaa cgcgcgcgcg
     1141 gcctgatggc gcgctatgcc atacagcagc gcgtgaccac cccgcaccag ctcgaaggtt
     1201 ttgacctgga gggctatgct tgggcccccg gcgccagcac gcccgagcgc ctggtctttc
     1261 gccgccaagc cacttgagga ggagcccccg ccatgagcct gtcccagacc atcacccccg
     1321 agctgcgcca gtggatcatc gaccaggcca gcgccggcgt ggccgcgccc gcgctgatcg
     1381 agggcatgcg cgcctcgggc tggaatgagg acgtggccat cgccgcagtc gagctggtac
     1441 tgcgcgcgca tctggacaag gtggcgctgg acaagggcct gccgccggcc gcccccgtgc
     1501 ccgatccggc gcttgccgaa tcgcccctgc acatcgacgt gggcgaccgc caggtcgagg
     1561 tgctgatggc catggcgaat ccgcgtgtgg tgctgtttgg caacctgctg tcgcccgagg
     1621 aatgccaggc catcatcgac gccgccggct cgcgcatggc gcgctcgctc accgtgcaga
     1681 ccgccaccgg cggccaggag gtgaaccagg cgcgcaccag cgacggcatg ttcttcgaac
     1741 gcggcgagaa cgccgtcgtc gcccggctgg agcagcgcat tgcccgcctg gtcaactggc
     1801 ccgtacagaa cggcgagggc ctgcaagtgc tgcactaccg ccccggcgcc gagtacaagc
     1861 cgcactacga ctacttcgac ccccaggaag ccggcacgcc cagcatcctg cagcgcggcg
     1921 gccagcgcgt ggccacgctg gtgatctacc tgaacagccc cgccaagggc ggcggcacca
     1981 ccttccccga cgtaccgctg gagatcgccc cgcgccaggg caacgccgtg ttcttcagct
     2041 acgagcgccc ccaccccagc acccgcacgc tgcacggcgg cgcccccgtg accgagggcg
     2101 agaaatggat cgccaccaaa tggctgcgcg agcgtgagtt caagtaaaaa cagcctgtag
     2161 cgcttgatac acaagcgctt gcagctatgt aatcgatagt aaacacatga gtacgccgga
     2221 acaatcccag ctcggcaagg ccagcgccta tgccgaccag tacgacgcag cgctgctgtt
     2281 cccgataccg cgccagccca agcgcgccga gatcggcatc gccggcgccc caagcttctt
     2341 cggcgccgac ctgtggacgg cctacgagct gtcctggctc aacctgcgcg gtaagccgca
     2401 ggtggccatt gcacacatca ccgtgccctg cgagacgccc aacatcgtcg aaagcaagtc
     2461 cttcaaactg tacctgaaca gcttcaacaa cacgcgcttt gccagcctgg atgaggtacg
     2521 cgcgcgcatc cgccaggacg tgagcgaggc cgcctggcgc ggcgccgcac atgccggcac
     2581 cgtgggcgtg cgcctggtca cgcccgatca gtttgacgcg gagcaggtgc acgagctgga
     2641 cggcctgctg ctcgaccgcc tggacatcga gtgcacccag taccaaccgg cgcccgagct
     2701 gctcaaggcc aaccacgacg aggcaccggt caccgaaacc ctggtcagcc acctgctcaa
     2761 gagcaactgc ctggtcaccg gccagcccga ctggggcagc gtgcagatcg cctacagcgg
     2821 cgcgcaaatc gaccaggaag ggctgctgca gtacatcgtc agctttcgca atcacaacga
     2881 gttccacgag caatgcgcgg agcgtatctt catggacatc tggcagcgct gccgccccat
     2941 caagctggcc gtgtacgccc gctacacgcg ccgcggcggg ctggacatca acccgctgcg
     3001 caccagcgcc ccgatgccgc tgccggccaa ccggcgcacg gcgcggcagt agcatcaggg
     3061 acaaaggcgc gggttcatgt caaagttgcg atgaaccgga gaacccaatg aacacgcctc
     3121 cccgcatgcc cacgctgtat atcccgcacg gcgccggccc ctgcttcttc atggactgga
     3181 acccggccga cgcctgggac aagaccgcgg cctacctgcg cagcattgcc tccagcctgc
     3241 caacgcgacc gcaggccatc gtgctggtca cggcgcactg gctcggcgcc cagccctgca
     3301 tgagctctgc cgcccggccg ggcatgctgt tcgactacta cggctttccg ccgcacacct
     3361 atgagctgag ctaccccgcg cccggcgcgc cggcgctggc cgggcgcgtc agcggcctgc
     3421 tggccggcgc aggcttcgac cccgtgcagg acagcgagcg cggctacgac cacggcagct
     3481 tcatcccttt gctgctggcc ttccccgatg ccgacattcc ggtgctgcag ctgtcgctgc
     3541 tgcgcagcct ggatgcggcc gagcacctgc ggatgggccg ggcgctgcgc cccctgcgcg
     3601 atgagggcgt gctgatcatc ggcagcggca tgagctacca caacatgcgc ggctacggcg
     3661 acccgcgctc aggcccggcg gccgccgaat tcgaccgctg gctcagcgag accgtggccc
     3721 tgcccaccgc cgagcgcgat gcacgcctgg ccaactgggc cacggccagc ggtccggccg
     3781 ggcgtctgtc gcacccgccg cgcgccgagg agcacctgct gccgctgcac gtggcggccg
     3841 gcgccgcagg ggaagggcgc ggcgccaagg tgtttggcga ccgcgtcatg ggggttgaca
     3901 tatcggcctt ccgtttcgat tgaaacgccc tccggacaag cgcaacatgc tatcaatacc
     3961 agagttaatc catgcacctg ggctccctgg atcgcgccat cgatcacatc gggctccgcc
     4021 tctcttccct tgtcttcggc ggcaatgtgg tcggctggac ggccgacctg gcgacctcaa
     4081 gggcagcgcc cgcagccact gggtcaaggc ctgccgccgc ccgcgcgtcc tgcccgtgcc
     4141 ggccgcgctg gacgccgtgg ccgtgcgcca cggcatgcgg cccggcgagg tgacccccgc
     4201 cttggtgaga gcgcgccccg ccgtcgtggc gcccatcacc ggcgccaccg gcctggagcc
     4261 actgggggga ctgatccgcg ccacgcggct gcgcctgagc gccagggaca tggccacatt
     4321 cgacgccgcc ggtgcggccg aagcgccgtg aacggcatga ccggccgcga tctggtaacg
     4381 cttcaaattt tggagcatgt tgcgctgatt cagagccgcc cagggggcga ttcatccata
     4441 gagccggcaa gcgttgcggc gcccctgggc cgggccgtcc tggcggccac gctgcgccac
     4501 gacccgccca cgccgctgga gctggagcac gccatcgagc agatcgagga cgcgttgatg
     4561 ccactgcgcg cgcaagtcgc cggggcgcgg ctggtgttcg ccaacgcagc cctgcatgcg
     4621 ctggcgcgtg ccggcgctgg cgcgaacggc tccgcaccgg gcgcggacat cacgctggac
     4681 atggtcgacg tcgaaacact gttcacccgc ctggcagccc gcgccaacgg ccggccggcg
     4741 acgcaagact gcctgcccac cgaccccgcc agcagcgcag cgctgctcgt cgcgcgcgaa
     4801 gtgctgcacc actggggctt gggcgcgctc acggtgcgcg acgcagcggc gtagccccgg
     4861 atcagcgcgg ccggtgctca gaacagcgcc tcgccctcgc cctgtgcctg ccgcccaacc
     4921 tcggactgcg gcagcagctt gcccacgcgc acccccagca aacgcaggcg cttatccaag
     4981 ggcacgcgtt tgaggcacag accggcctcg cggcggatga cggtggcgtc ctgcgtcggc
     5041 aggacgatgg tgtggtcgcg cgtggcaaca cggaaatcgt cgtagcgcag cttgatgcct
     5101 atggtgcgac ccgcgtagcc cttgcgctgc aagtcggccg ccacctgctg gcacagctcg
     5161 gtgaagatct ggcccagcag cgccctgtcc tgcaccgcat gcaggtcgcg gtcgaaggtc
     5221 gtctcgcgac tcatgctgac gggctcgctc tccagcacca cggggcgatc gtcgcgaccc
     5281 caggcggcat cgtgcagcca ggcgccggtg gagcggccaa aatgcgccac cagccagtcg
     5341 cgcgggcgcg ccgccagctc gccaatggtg tgtatgccgt aggactgcag cttctcgtcc
     5401 gccttggggc ccacgccgtt gatcttgcgg caggccagcg gccagaccag ggtctgcagg
     5461 tcatcgggtt gcaccgtggc gatgccgttg ggcttgttga actcgctggc catcttggcg
     5521 atgagcttgt tgggtgccac gcccaccgag caggtcaggc ccgtggcctc gaagatgctc
     5581 ttctggatca gccgcgccag cacccggccc ccttcgcgct ggccgcctgg cacctgcgtg
     5641 aagtcgatgt agacctcgtc cacgcccctg tcctccatca ccggtgcgat gtcggtgatg
     5701 acctgcttga agcgccgcga gtagcgccgg tactcggcaa aatccaccgg cagcaggatg
     5761 gcctgcgggc aggcacgcgc cgccttcatc agccccatgg ccgagcccac gccaaactgg
     5821 cgcgcggcgt aggtggcggt ggtgatgacg ccacgaccca cgtagtccgc aagacgagga
     5881 aaggccgcga cgggaatgtc ggccagccgc tcgccgtact gcgcggccag ggcgtcgtcc
     5941 tgctgcctgc gcccgccgcc aatgaccacc ggcaggcccg caagctgcgg gtagcgcagc
     6001 aactccacgg aggcgtagaa cgcatccatg tccaggtggg cgatgcggcg ggtcgggcgg
     6061 ggcaggtcgg cggcggtcac ggcgaggatt gtcgccctca aagcccctgc aggctgatca
     6121 gcaccagccc caccagcatc agcgccatgc ccagcttctc gggccgcgtg aaggactcgc
     6181 tgagcaggcg gcgcgagacc aggtagctga acaccacctc caccatgccc agcgtgcgca
     6241 cctcggccgc gccctgcatg gcgtaggccg tgaaccaggc cagcgacgcg gccgcgccca
     6301 tgctgccggc cacgagcgac agccgccagg cgggccacag cggcgccagg cctttttccg
     6361 atcgcagggc cacccaggca cccaggccca ggctctgcag cagctgggcg atgaccacgc
     6421 cccaggcgcc cgacagccag ggcgtctccg cccccagtgc cagcgcgcca ccgcgaaagc
     6481 ccacgctggc aatggcaaag ctggcgccgc agcccaggcc atagagcgcc ggacggctga
     6541 gccaggccga cagcgacaac agctgcccgc gcggcggcag cgacagcagg aacacgcctg
     6601 tcgtcgccac caccatggcc agcagcgcca gcggccgggg cagctcgtgc agcagcaccg
     6661 tggcgaacag cgccacctgc agcacctcgg tcttggacag ggtcacggcc acggcaaagt
     6721 tgcgctcggc catggccagc agcagaaagg ccgtaccggc gatctgaaag aaggcgccca
     6781 gcgcgatcca gcccaggtag gcggcactga aatgcggcag ggccggcggc tggtcgggcc
     6841 agcagtacag cagcgccagc cacaggccgg caaacggcag gccgtagaga aaccgcacca
     6901 gcgtcgcggg cagcgtgccc agctcggcgg tcaacgtgcg ctgtgcggtg ttgcgcgcgg
     6961 tctgggccag ggtggcgaac agcaccaccg gcacccagag ccaggcgggc gcttcaagag
     7021 acaaagacat gggaggacgg catgacgcag gccccgcagc atagccagcg ccccgaccgc
     7081 gcgcgccgcc gattggcgag ggccaccgca atgcttggcg atggcggccc ggctatggac
     7141 atggctcggg agcgccggcg tctcgccggc attcacggtc aaatgccggc gagacgccgg
     7201 cgctcctaga tataaaacgg ctgtatcgct tatgcagcaa gcgtcataag ctatcaaaac
     7261 agaagttcca tcaaccccgc ggcgccaccg ggtagctgcc cggcacgagg atggagcggt
     7321 ccacgttctg cagattggta tggccacaga aggccatgct cacgtccagc tccttgtgga
     7381 tgatttgcag ggccttggtc acgccggcct cgccaaaggc gcccaggccg tagaccatgg
     7441 cgcggccgat catggtgccc ttcgctccaa gcgcccaggc gcgcagcacg tcctggccgc
     7501 tacggatgcc gccgtccatc cacacctcca gcttgtcgcc cacggcgtcc acaatcgccg
     7561 gcagcgcggc gatggacgag ggcgcgccgt cgagctggcg cccgccgtgg ttgctgacga
     7621 cgatggcgtc ggcgccgtta ttggcggcca gcagcgcgtc ttccacctcc atgatgcctt
     7681 tcaggatcag cttgccgccc cactgctgct tgacccagcg cacgtcctcc caggacaggc
     7741 gcgggtcgaa ctgctcattc gtccaggcgg ccagcgagtt catgtccgac acgcccttca
     7801 catgccccac caggttgcgg aaggtgtggc ggcgcgtgcc ggccatgcca aggcaccagc
     7861 gcggcttggt catcaggttg atgatgttct tgagggtggg gcgcggcggc gcgctcaatc
     7921 cgttcttgat gtccttgtga cgctggccta tcacctgcag gtcgagcgtg agcaccaggg
     7981 cgctgcactt ggcgtctttg gcgcgctgga tcatgcgcgc catggcgtcg cggtcgcgca
     8041 tcatgtagag ctggaaccag aacggcgcgc tggtgtgctc ggcaatgtcc tcgatcgagc
     8101 agatgctcat ggtcgacagc gtgaacggaa tgccgaactt ctcggccgcg cgcgcggcgt
     8161 ggatctcgcc gtcggcgtgc tgcatgccgg tcaggcccac gggggcgatg cacaccggca
     8221 tgctggtctg ctggccgacc atggtggtgg cggtgctgcg cccttccatg ttgacggcca
     8281 cgcgctggcg gaactggatg gcgtggaagt cgctttcgtt ggcgcggtaa gtgccctcgg
     8341 tccacgagcc ggagtcggcg tagtcgtaga acatgcgcgg cacgcggcat tctgcgacct
     8401 ggcgcaggtc ttcgatgcag gtcattttgg aaagatcggc catggcagga gggggtaaca
     8461 acaacaagcg cgcatcgtag ccctggcggc cccggccgtg gttgaaggct tttgccccgg
     8521 ctgtgaaaat tttgcagccg cactaccatg gcccccatga tcaccctcta caccgccgcc
     8581 acgcccaacg ggcacaaggt ctcgatcgcg ctggaagaac tgggcctgcc ctaccgcctg
     8641 caggtgctgg atttatccgc cggcgagcaa aagcgcccag agtttctggc catcaacccc
     8701 aacggccgca tcccggccat cgtcgaccac gatgccggcg actttgccgt gttcgagtcc
     8761 ggcgccatcc tggtctacct ggccgagaag accggccgcc tcatgccgca ggacgcgccg
     8821 ggccgctcgc gcgtgctgca gtggctgatg ttccagatgg gcggcgtcgg ccccatgatg
     8881 ggtcaggcga atgtgttcta ccgctacttc cccgagaaga tacagccggc catagaccgc
     8941 taccaggccg agggccggcg cctgttcgag gtgctcgacg gccacctggc gcagcacgaa
     9001 tggctggccg gcgactactc gattgccgac atcgccaact gggcctgggt gcgcacgcac
     9061 cgctggtcgg gcatctccat cgacgggttt gatcatctgc gccgctggcg cgatgccatc
     9121 cgcgcacgcc ctgccgtgca gcgcggcctg ctgcagccgc cggccaagat ggacctggag
     9181 cgcgacggcg aggccggcgc ccaggccttt gccgcgcagg cgcgcaccat ggtcatcacc
     9241 ggccagcagg agaagccatg aagctctaca ccgcccagcg cgcgcccaat ccgcgtcgcg
     9301 tgctgatgtt catccacgag aaggccatca ccggcatcga gctggtgccc gtcgacctga
     9361 acgccggcga gcacaagggc gccgcctacc gtgcgctcaa cccagtggcc accgtgccga
     9421 cgctggagct gcccgacggc cggtgcttaa gcgaaacgcg cgccatctgc agctacctgg
     9481 agggcctgca gcccgaaccc aacctgatgg gccgcgactt tgccgagcgc gccttcatcg
     9541 aaatgcacga ccggcgcgta gagtggtact ggctgctacc gattgccaac tgcgtgcgcc
     9601 acacccaccc cggcctggca acgctggagc gcccgcagtt cgccgacttt ggccggtcgc
     9661 agggcgagaa ggtgcgcgag cgcgcgcgct ggctcgacga gcagctggca gagcagccct
     9721 ggatggccgg cgagcgcttc acaattgccg acatcaccgc cttttgcgcg ctggaatttg
     9781 cccgcggact gatgaagttc aacgccggcg ccgagggctt tgcccggctg caggcctggc
     9841 gcgagcgcat ggccgaacgc gagagcgcca gggtgtttta gtgtcctgag ttgaaggttc
     9901 gttgaataac aaatcgaccg atatcggcac caggcaaggc gcaaaccgca gccatagccg
     9961 tagctatggc gaggatttgc aacgccgcat ggcgtcgata gtcgggtgat ttgttcatcg
    10021 aatttccaat tcaggacact cattcacgcc tggggccagg gcgtgtcgcc atacagttgg
    10081 gcggcgctga taccggtgtc cagccgcgac agcggcaggg gaggcggcac caacggcagg
    10141 cgcaagcagc ggtaccagcc ctcgttggac agcccgtagg gcgtggcgtc ctggttgtcg
    10201 aaagcgtaga acacctgccc gcagccggcc atcagcagcg ccgccagaca catcgggcag
    10261 gggtggccgc tggcgtacag ggtgcagccc gccaggctgg ggctggcctg gcgccgggtg
    10321 ccggcgcgca gtgcctgcat ctcggcatgg gcactgggat catgggtgtg cacgatgtca
    10381 ttgatgccgg tggcgatcac ctcgccatcg cgcgccagca cggcgccaaa cggccgccca
    10441 ccacgctggc ggttggcatg ggccagatcg accgccaggc gtagcaactc ggtatgggat
    10501 ggcatgcaat acctccgatg gattgggtgg cgcagggcaa attgtgccgc gctcacggca
    10561 tggcgccgcc cgccccgccc ggccgccctg ggccgggctt gacacccgcg ccgcgcacct
    10621 cgatctgcac gctgtcctgc atctggcctt gcgcgtccag cagctccacg cggtggcggc
    10681 ccggccaggg cagccaggcc agtgcagcgc cctgccccac cggcctgccg tctatgcgcc
    10741 agcgcagcgc agtgccgccc tgggcctgca gttgcaggcg ctggtgcgcg ggcggtatgt
    10801 cggggtccag cgcgatgatg ctgcccggcg ccggggcgat gatgcgcgcc ggcgtaccaa
    10861 aattcatatg ttttttgccg ctagcgcttg ctatataagc gtcaggagct tctatatcaa
    10921 tagcaaatac cgcctgctgc gtgccacgca caaaccactc ctcgcgcggg ctctccagcc
    10981 ggtcggcgcc gccgtcaaag cgcaccggct ggcgcagcac gcccgccggt ggccgcggcg
    11041 cgtggctggg cgcggcgcga tgcagccagg ccatgacatc ggcccagatg ggtgcggcgc
    11101 cgctgctgcc gctgacatcg tgcatggggc tgccgccggc attgcccacc cacacgccga
    11161 cggtgtagcg ctgcgaccag cccatggccc agttgtcgcg catgtccttg ctggtgccgg
    11221 ttttcacggc gctccagaag cgcgtgttca gcacgctgtc cgtgccaaag ctgggcacgc
    11281 gggcgttgct gtcggccagc atgtcgccga cgatgaaggc cgcgcccgca tccagcgcct
    11341 gtgtcagcac gggacgtgcg cccggggcat aggccaccgg gctcaggcgc ccgccgttgg
    11401 ccagcgcccg gtaggcgttg gtcagctcca gcagccgcac ctcgccgctg cccagcgcca
    11461 ggctgtagcc gtagtagccg ccggactcgc gcagcgccag gcccaggcgg cgcagctgcg
    11521 caaagaaggc atcgggcgac accatgacca gggtgcgcac cgccggcacg ttgagcgaag
    11581 accccagcgc ctggcgcacc gacacccagc ccttgaactg ccggtcgtag ttctgcggga
    11641 tgtacaggcc gccggcggtg gcgatctgcg cgggcgagtc gtgcagcagc gaggctgcgg
    11701 tcaggcgctt ctcggcaatc gcctgcgcgt acagaaaggg tttcagcgtg gagcccggct
    11761 ggcgcagggc gagcacgcca tcgacctcgg ccgcctggct caaggggcca gaggagccga
    11821 cccaggccag cacctcgccg ctggcgttgt ccagcaccac cagggcggcg tcctgcacgt
    11881 tgcgcccggc gagctcgcgc aggtggcgct gcagcgtctg ctgcgccatg cgctgcagcg
    11941 gcgcgctcag gctgctgcgc tggccagcgc catggccgcc gcgcagccac tggcgcgcaa
    12001 agtgcggggc gatgccggcg ctggcgtcat aggcacggcg ctgcaaggcg gcggtggtaa
    12061 acaggtctag cgcctcgcaa tcggcctggc cttgctgcat gtcgcgcagc acgccgcagg
    12121 cgcgccgcgc caccagggcc ggcctggcgt tgggcgcacg caccagggct gcggcgacgg
    12181 ctgattcgcg ctcgtccagg ccatgggcgg ccttgccgaa cagggtgcgc gccagcgcat
    12241 cgatgccgac cagctcaccg cgaaacggca ccaggttcag gtaggcctcc aggatctggt
    12301 ccttgcgcca gcgccgctcc aacagctgcg cggccacggc ctggcccatc ttctgcgtga
    12361 ccgtgcgccc gcccgggccc tggcgccagt caccgtccag caggccggcc agctgcatgg
    12421 tgagggtgct ggcgccgcgc gtgcgctcat gccacaggtt ggcccaggcg gcggccgaca
    12481 ccgccgccca gtccaccccg ctgtgctcgt aaaagcgcct gtcctcactc agcaccaggg
    12541 cgcgctgcag cgcgggcgag acatcggcca gccccagcca ctggccacgg cgcaccgtgg
    12601 cgtcggtgcg caggcgctgg atcacctcgc cctcgcgcga gagcaccaag gtttcggatg
    12661 agcggaagtc cgcgcgcacc tcgtcaaagc tgggcagggc cagcgccggc ccggccgcca
    12721 gcgccaaccc cgccgcccag gcgcccaggc gcatgcagcg caggacgcat cgaactacgg
    12781 acggaacaaa cagggacgcc ggcatgggcg ccgcagctta tcacccaagc ctgcggccgg
    12841 gcggcccttc agccggcgtg cgccacgccg tactgcgccg acagatagcg gcggatctgg
    12901 tctggggcat caatgaagcg aaccccgttg tgcgtgcgcg tctccacgcc ggcatcggag
    12961 accacccgcc cgtggcccag caccagcacg ggggcggact ggttgtccgc gccaatcagc
    13021 gcgacgatgg ccgcgcgcgg acgcggcgcg ccgatgtagt gcacatcgac ctcattgcgc
    13081 agctgcggga aaaagctcag cagcccctcc acggccaccg agtcgccaca gtagaacggg
    13141 ccttcggact ccttgaagaa gccgggctgg aggagaaaga gctggtcttt tggcatgggg
    13201 ttccttgcat caatggttca gtggggtgga cggtgcaggc acttgagcta catcgtggtt
    13261 gccatggtag gaatcacgcg ccgcgcagac gagtggcatg aatgacaact tgcgtcacaa
    13321 tcgtgccaat atggatacgc cccacaccgc cccgggattg gtcgccgtcg tcgtctacaa
    13381 cggcctgagc ctgttcgagt acggctgcgc ggtcgaggtc tttggcctgc cgcggcccga
    13441 gctgggcgat cgctggtacc gctttgccgc ctgcgcggca gagcccggcg tgctgcgcgg
    13501 cgcgggcggc atacagatcc agcccgacca gggcctggag ctgctggcgc aagcgcgcac
    13561 catcatcgtc cccggctggc gcggcatcga tgcgcccgtg cccgagcccc tgtcccaggc
    13621 gctgcgccag gcccacgccc gcggcgcacg cgtcctgtcc atctgcaccg gcgccttcgt
    13681 gctggcggcg gcgggcctgc tcgacggcag gcgcgccacc acgcactggc gccatgcgca
    13741 aacgctggcc gcccgctacc cgcgcgtgca ggtacaggcc gacgtgctgt acgtggacgc
    13801 gggcgacatc ctcacctcgg ccggcagtgc cgccggcata gatctgtgcc tgcacctgat
    13861 ccgccgcgac ctgggagcgc gcatggccaa ccaggtggca aggcgcctgg tgatgccacc
    13921 gcaccgcgag ggcggccagg cgcagtacat cgaggagccc gtgccccggc gcagcaacgc
    13981 atcactggcc ccgctgctcg acgccatgcg cgccagcctg ggccaggact ggcccatcgc
    14041 caagatggcg gcccacagcg ccaccagcgc gcgcagcttc cagcgccact ttgtcggcgc
    14101 catgggcatg ccgccgggcg aatggctgct ggcgcagcgc ctggccgccg ccagaaccat
    14161 gctggaggaa accgatgcca gcgtggacga gattgcgctg caggtcggct ttggcgacgc
    14221 ggccacgctg cgcggccatt tccgcaaccg gctcggcacc agtcccggcg gctaccgcaa
    14281 gcgcttcagg gcataggcgc ccgcagcccc ccatcggcgt gacattgcag agggagcctg
    14341 gcccaatgca caggcccatg gactgcaatg ccacggtacg ttaggcgccg gccctgcgtc
    14401 gtcacccaga gccggctacc tgcacaccac tgtaggccgc cgccgtgaca gctttatgac
    14461 ggcctggccg cggcctgcgc cttgcccggc gcgccaaaac cctcggtggc cagttgcacg
    14521 ccatcgtcga acaccagggt gcgttcgggc cagcgcagcg ccgccagctt gaagcggcgc
    14581 gccgggtctt cgccgttgcg acgcgcggcc cagcgcgcgc ccaccgagta gtccacgcag
    14641 aacacattgc cacgggcgcc cagccaggcc agcggcccgg tatggccaaa caggttctcc
    14701 gcctgcggcc catgggccgc cgcatcgccc gccagcgggc ggcgccagta atggccgacg
    14761 acgacggccg gggcctcggt gtattcgctc caccaggcga cgcgctgcac gaagcgccat
    14821 ttgccgccgg catagaaggg cgtgcggcac tcgcgctcca cccccgtggt cagcacctta
    14881 agcgggttca ccatggcctt gcccagctcg cgctcgctgt gcgccggcag aaagggcggg
    14941 cgctgcgcgc catcctccag gctgtgcggc cagcgctggc gctcggtctg catgcgctgc
    15001 agcacctggc tgctgcgcgc ctgctcggcc gcctggtcct catagcggtc gtaggcatgg
    15061 cgtacatcgc ccaccggcag cgagcgcgcc atggcgatct gcggggataa ccaagcggcg
    15121 tgcaccacgc gcaggtcctc gcgctccagc gcgataggca tttggttgag ttgctgcagc
    15181 agggtcgccg catcggcctt ggacagacgc gcaaacggcg cgtacttggg ctcgtcggac
    15241 gccagccggc tgtcaaagaa ccagcccgag ccatccttgg cgtcctcgcg cagcaggttg
    15301 atctcgtggt tgcccagcac cgccagcgcc gttcccgccg cgatcatgga caacaccagg
    15361 cgcagcaccg ccgggctatc ggggccgcgg tcgcaaaaat cgcccacgaa caccagacgc
    15421 cggccctggg ggtggtggcc ctgttcgtcg tagcccaggt gggtgagcaa ttgcctcaag
    15481 gcggcgatct cgccgtggat gtcaccgacg atgtccaggg ggcctgaggg caggcattga
    15541 atgaggctca tggtttggca aagggaatgg ggcgaactca agcgggggtg cgcgcaaatg
    15601 gcatcggtgt ggtatgtctt tcgcatcacc tcgccaaggg cttcctatcc gtcattccat
    15661 ttctaaatca tccgcgtcgt tgttgcttcg ccttgtcgtg ctacagcact gcctgcggct
    15721 tcgcgcctag accggaatga tttggaaacg gaatcagcta tacacgcccc gccagatgca
    15781 tggacgcgcg cagcatgtcg ttgatacgtg tttgccagcc atcaccggtg gcgcgcaagg
    15841 cggcaagcag gtcggcatca agccgcagct tgaccgcctc cttggtcacc gccgccttgg
    15901 ggcggccgcg gcgcaccagc ttctcgccga catattcatc ggcctgttcg aaaaatgcgt
    15961 ccgtcagctc tggtgcatcg tccgggtcaa cccaggcggt gggcatagcg tgcttgttcc
    16021 cgttcattgg ctttcctcat gctgatgatg cggcgcgcct cgccgcgagg ggtccagacc
    16081 atgaccacca tgcgcccatc gagttgaccc accgtgatgt agcgtggctc gctgtagccc
    16141 gcacgctcat cttctgccgt gaagtgcctt gcctcaaaga cctccgccgc gcgagaaaaa
    16201 tccaagcccc gttcggccag agccttgtcc cgtttgtcgg gatcaaactc gatcaacact
    16261 ttttattgta cccccaataa ataacagaac cgacctgact gaggcacccc gtttcattca
    16321 ggagctaccc gtacccgcgt attcggcgcc tcgccaaaca tctcgggcgc gtacagcgcc
    16381 tcgacgcggc tcgccggcag ggcgaagtcg cccacgttgt tcaggcgcag ggtgtattgc
    16441 atggtgaccg tgcccttggg caggtattcg tagtaggcgc gcaaggactc gaagctgcgc
    16501 tcctcgaacg ccggccagcc ggggccctgg cgcttttcgt cctgggtggc gatctcggag
    16561 tcgcgcccca ggccgctgcc gaggatggtg gcgccggcgg ggatcgggtc ggtgatggcg
    16621 acccagctca tgtcggcgct tgccgtgacc tccagcgtca cgcgcagcac atcgccgcgc
    16681 gaataggttc ccgcaggccg agtcttgtcg gcctgctcca cgggcgtgac ggttctcttg
    16741 atggcaaaac ccgcgctgaa gggcgccttg cgctccaccg ccgccacgga ctgcagggtg
    16801 agccagggct tgcccgtgcc ctggtgcgtg acggtcaggc tttctgcgcc cttgccccag
    16861 ggcaggaaca tggcgttgtt cttcagcccg ccgggcgcgg cgggtgcgcc aaaccaggtg
    16921 gtctgatggg cggcgccacc gctgtcggcc gccgtgatgc gtgccacctt gccccaatcc
    16981 acgctggcgg tattgccgcc caggctggcg cgggtgctgc cagccacggg cgtggcctcc
    17041 aggctggcgc tgaatttctc cagcgccagg ccgccccaca ggttggccgt ggtggtatgc
    17101 caggctccgc cctgctggcg cgcgatgaag ccgctggcaa gacggcccag atcatccttc
    17161 cacgctggat cgtccagcac ggtgagcagc aggcgcgcgc cgttcacgtc gccgccctgc
    17221 atcagccacc accaggtgtc accctgttcg gtgctgaagc tgagcctgct gccctggtag
    17281 gacaggcgcg cgcgcaggat ctgcatggcg tcccgcaggc gctccgcgcg ttggggtacg
    17341 ccgtccatgc gcttgaggat gttgatccag tcgatgagcg cgtgcgtggg ccactggttg
    17401 ggcgcgatgg tgaggctggt cagcatgcgc ggctgggcct tggcgtagcg cgacagggcc
    17461 tcgatggcgg cgagcttgcg catgtccagg tctttgcgcg ggctccagaa gtcgcgctct
    17521 atgcggccct cgacgaaggc gatcaggccg cgttccatgg cttcgcgcag ctcgggcggc
    17581 agggcgaatt cggggcgcag gccggcggcc tcgtggctgc tggccagcag gtaggcggtg
    17641 agcgtgtcgc tgccgcggtc ggcagcgcca tcacgcggcg gaaagtaata cgccaggccg
    17701 tcgccatcca gatagctggg cagctgcgcc agcacggtct tccacaagcc ggcgtcggcc
    17761 aggcccatgg ccttgctggc cttttgctcc aggcagctga acgggtagtt ggcccaccag
    17821 tcgcgcacgc ctggcaggcc atcggccagc ttcggtgtca acgacagctt caggccgccg
    17881 cgcccggcga tggcgtccgc gggcggggtc acttccacct ggtagccgcc atccacctgc
    17941 accagccggg cctgctgcac ggccagcggc acggcgggta tcagccgctg gctggccttg
    18001 agggcatcgc gcgcgccgcc cagcgtgtcg cgcgcctcta tctcccacag gatggcctgg
    18061 gcgcgcattt gccccagttg ctcgggcgcg gtgacgttcc aggccagttc gcgcgcttca
    18121 cccgggggga tggccacggt ctgcctgtcc aggctaagca gcgtggcgcg tggcgccacc
    18181 tccacccgca tggcgcgggc tgtggtgttg cgcagcgtga tctgggcgcg aaatgcgtcg
    18241 ccgccccgca ccagcggcgg caggccgctg atgatctgca gatcctgtgc gctgcggatg
    18301 ctggcgctgc cggtgccgaa cagccccgtg ccggcatcgg ccacagccac gatcttgaac
    18361 gtggtcagcg cgtcattcag cggcaccgtt acctttgcct ggccccgggc atcgagcttg
    18421 atggccggtt gccacaagag cagcgtatcg agcagttcgc gcgtgggcgc gcgcccaccg
    18481 ccgccgcccg cgggaacggc cttcttgcca tagtggcgcc ggccgatgat ctccatctgc
    18541 gccgtggacg tggccacgcc ccaggcgcgg cgctgcagca tggcctccag caggttccag
    18601 ctggtgttgg gcatgagctc cagcagcgcc tggtccacgg cggccagcgc cacctctgca
    18661 cccgcagccg gcttgccatt gggcagcgtg ccttgaatag tcacctgggc cttgccgcgc
    18721 accgggtaac tgtccttgtc ggccttgacc ttgacgtcga tgcggtgcgc ctcgtggccc
    18781 acgcgcagct cggccacacc caggcgatag gcgggcctgg acagatccac catggccgtg
    18841 ggcgcggcgt actcgcggcc ctcgtaccag aaggcgctcc accaagcgcg cggcgccttg
    18901 tagccccagg tgaacaggct gtaccagggc acctcgtgca gacgcccgcg cagcgccagc
    18961 acgctcacat agacgttggg cgcccagccc tcctctatct tcagctgcag cgtcgggtcc
    19021 tggcccgaca gctgcaccac gcgggtctcg atcacgccct cgcgctccac gctgaccagg
    19081 gccgtggcct ggcgaaacgg catgcgcagc tgcaggcgtg cggtctcgcc cggctggtag
    19141 ctcttcttct cgggcagcag gtcgatgcgg tcgtggtcct cgccgccaaa ccacagctcg
    19201 ccgcggcgcg tgacccacac cgacgaggcg gcctgggcct cgttgccgtc cttgtcgcgc
    19261 gcggtgacga ccagttccac ctcgccggcc tcttccagcc gggcgtcgca cagcagcagg
    19321 ccgcgcgcgt cgctcttgcc ggtgcacagc gtgcccaggt ccttggtctc gacacggttg
    19381 tcgtagctgt agaagccccc caccatgcgc tttctgctgc tggtggtgct gcgcgcaatg
    19441 gcctgcacgg ccagtggcac gcccgcttgc gccttgccat cgagcgacag ggccagcgcc
    19501 tgcaggcgca ctttggagcc gctggacacc cagccctcgg tcttgacgcc cgccaccacg
    19561 gcggcgggcc acagcgtgct ggtgctgcgc agggtctgca gctcgccatt ggggtcggcg
    19621 tagctggcct ccagcaccag atcctgggcc tgcgcctggc gcgcaatgtc gtcaatgtcg
    19681 agacggccgg tgccgtttgc gtccagcatc aaaggccgct tgtcggccac cacgcgctgg
    19741 ttctggctgg cggcgggctc ttcctcatcg ctgttgttca ccgcatcacg cttgcgcggc
    19801 ggggcaaagc tgaaggcttc gaaatcggca aactgcagcg gcttgggcgc caccatggcc
    19861 gacacgcgca ccggcaggcg tgccgccggc ccgccggaga catagctcac ctgcacgtcc
    19921 accggcacgc tgcgcgcgcg caccaggggc ttgccggcca cgggcgtcac gcgcccctcg
    19981 aacaccggca ggcggaattc ctccacgcga aagctgcccg actgctggcc gccgccctct
    20041 tcgctggcgc gcagctccac ctggtactgg cccagccggg cggccgctgg cacggcaaat
    20101 tcactctcgg ccgacagccc gccggtggcc gtgttgcgcc acttgagcgg ctgctggtac
    20161 tgctggccgc tgcccacatg ggtgatgacc agggtgccag gccgctgcct gggcagggca
    20221 aagccggcgc cggtctgcgt gcgcagcaca tgcttcatcg acagggtttc gcccgcgcgc
    20281 agcaaggtgc ggtcgaagat ggtgtgcgcc acctcgtcgg ggcgcggcgc gctgctggtg
    20341 ggcaggttga agcgccaggg ctctatgccg cgctgccagt cgctccaggt aaaggccatg
    20401 tcgtccccgg cgcgcgccga gacaaagtaa gcgttgcgcc attcgccctc gccaccgcag
    20461 gccggcggct cgctcgccag gccggcgatc cgcgccacgc catcggcgcc ggtgctggcg
    20521 ctggccagct cacgcccgtc gcagctcgat acgcgcacgc gcgcgccggc cacgggctgg
    20581 cccttgtcca gcgtggtcac ccaggccacg gcgttctcgc gcccgagctt gaaatgcacg
    20641 cccaggttgg tcaccagtgc gctggtgcgc accaccatgc tgcgcgggct gccatggcgc
    20701 ccatccagca gcgactggcc cagcagcggc gaggcgatct ccaccaccga aaaccccggc
    20761 ggcagcggta tgcccaccac ctcgaagggg cgcgggtcgc ccttggtcgg ctggggaatg
    20821 tccagcgtcc gcacgcccgc ctggccggcc agcagggaca gcatgcgcgc ctgcacctgg
    20881 tcgccatccc gctcgtccag caccttgggc agcgggccct tcacgtcacg cagcgcctgc
    20941 ttgcgatcca cgtaatggtc gtcgtagcgc tgcagcttgc gcagccaggc aatgatgtcg
    21001 gcgtcgctat ccgcgcgcag cgtgcttacc ttgcccccct gccccgcgcc gggctgcagg
    21061 gtcttggcgt ccagttgcgc ctctatgttg cgcagcgtga ccggcagcag cgccgggccg
    21121 tccgggccct cggcgtagcg ctcgaccacg ccaaagggcg cggcggcaaa cttggccagc
    21181 ggcggcatgg ggccggtcgc caccttgagc ggaaagctgg cgacattgcc caggcggcgg
    21241 cccgatgcgt cctgaaagtc tgcgggcagc tccagcgtga atcgggtctg cgcggtcagc
    21301 ggcgcggcaa aggccacggc atcgaccagc gcatcgccct cctgcgcgcc gtccatggtg
    21361 ggcatcaggg tctggccgcc gcccttcagg cgtatgccct gggcctgctt gcgcgtcacg
    21421 ggcgcgttga atgccaggcg cagcggacgt atcggcagac agtcggactg ggcgttctcg
    21481 cgctcgcagg cgaattcggc cgtgaagggc tggcgcacct ggtaggcaaa gcgcttttcc
    21541 accgcattgg ccacgccgct gggcgtggcc acgccgcgcc cgtagaccag ctgcatcttg
    21601 ctgccggccg tgagccggcg cccgcaggtc agcgtcgcgt agcggagcgg ctccttggct
    21661 gccgccttgt ccagatgcag cgcgcgcagc agctcggtgc gctgcgcgcc ctcgaccagg
    21721 cgcaccggca cacgctcgcc cacgccatcg gccaagcacc acatatgctc ttgcaggcta
    21781 gcgacgctgg ccgggccatt gagctgcagc acaaagaact gctcctcgtc gatcgcctca
    21841 tgggtgccgg gccgtatgct ttgcacaaac gggccaccac tattaaattg atagctttta
    21901 gcgcttgaca gtagggcgcc agaggccgat ttgaaccctg gttttgcact gaccgtgcag
    21961 cgcacgccgg gcggcaagtc ctgcgcaaac tcatagaccc actcgcgctc gccgctccag
    22021 cggccctggc cccgagcagc atcggggtcg ctgcaggcca gcgtgaacgg cgccggcgcc
    22081 cgcgggtcgc ccaggcgcac ggccggcgcg tcgaacttgg cgaccacctg gcgcacctgc
    22141 gcgacctcgc cctggggcga gaggctggtc acggtgaggg cctgggccgc agccgtggcc
    22201 agaagaagca gagccgccat cgccccgcgc acgccgccat tcatgccggg atcctttgcc
    22261 aactcactgc ggcctgaagc cgctgtccaa gatgatgcgc cgccacaccg ccgtgtaagc
    22321 gcgttcgcgt gtcgtcagct gctcgggcgt catggtgccc acggtgaggc ccatggccgt
    22381 gagctgatcg cgcacctcgg ggatggcgac cacctgggcc agggcgtcgg aaaaacgctg
    22441 gatgaaggcc tgcggcgtgc ccgcgggcgc atagaagccg tagtacggcg tgtcctcgaa
    22501 gcccttgagg cccagctcgg caaaggtggg cacgtcgggc agc
//
LOCUS       DAOMTQ010000031        22356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_263_length_22356_cov_26.973992, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000031 DAOMTQ010000000
VERSION     DAOMTQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 22356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22356
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_263_length_22356_cov_26.973992"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PK518_04920"
     CDS             complement(<1..1683)
                     /locus_tag="PK518_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRM48287.1"
                     /translation="MKRMLINATQPEERRLAIVDGQKLLDYEIEVEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEISRQYFAEGVSPSQARINDVIKEGQELLVQ
                     VEKEERGNKGAALTTFISLAGRYVVLMPNNPRGGGVSRRIEGEDRAELKEAMDQLEYP
                     KGMSIIARTAGIGRSAPELQWDLNYLLKLWSAIDGAAKAGKGAFLIYQESSLVIRAIR
                     DYFNADIGDILIDTDDIYEQAQQFMAHVMPEQAARVKRYRDDAPLFSRFQIEHQIESA
                     YARTVTLPSGGAIVIDHTEALVSVDVNSARAIKGGDIEETATRTNLEAADEVARQMRL
                     RDLGGLIVIDFIDMEESKNRREVENRLRDALRQDRARVQFGTISKFGLMEMSRQRLKP
                     SLSEGAHIRCPLCDGTGHVRDTEGFALQMLRMIQEESMKDNTAAVHCQVPVEVASFLL
                     NEKRNEIAKIELRKRVAIMLIPNKSLATPHYKLERLKHDDPRLDGLEASYQLADVPEE
                     QTGITRRSQEPTNKQQPVIKGVLPDAPAPVAEPRPEAPARGQGQQRSAAAPTP"
     gene            2249..3250
                     /locus_tag="PK518_04925"
     CDS             2249..3250
                     /locus_tag="PK518_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM48288.1"
                     /translation="MKPIIEGKPSPTPAARIVEVDAESTGQRLDNFLIRHLKGVPKTH
                     VYRIIRSGEVRLNKGRTSADARVAEGDLVRLPPVRVSEKIAEKAERPAPAREFPILLE
                     DEQLIALDKPAGVAVHGGSGVSFGVIEQLRQARPQARFLELVHRLDRETSGILLVAKK
                     RSALTHLQDQFRGRETGKTYLALVTGSWPANHKVIDQPLHKYLQEGGERRVRVTTQDD
                     PDGMRSITLVKVREQLAPRPMAGLPAMSLLEVTIKTGRTHQIRVHLASQGHPIAGDDK
                     YGDFDLNRRLQKNGLKRMFLHAWRLQFDHPGSGERLELRAELPPDLADFLATEPTHA"
     gene            3243..3911
                     /locus_tag="PK518_04930"
     CDS             3243..3911
                     /locus_tag="PK518_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM48289.1"
                     /translation="MPELRPRRFDLIAFDWDGTLFDSTAIIVRSIQDAVRDVGGAVPS
                     DEAAAWVIGMALEPALAHAAPDVPREHYPQLANRYRYHYLKRQDDVTLFAGVLPMLHA
                     LRARGHLLAVATGKSRRGLDQALQLVQLRGMFDGSRTADETAGKPHPLMLQELMAEFD
                     VAPGRLLMIGDTTHDLQMARAAGCASVGVGYGAHDVAEFAALGPLCVVPSVAALHGWL
                     QEHA"
     gene            3911..4279
                     /locus_tag="PK518_04935"
     CDS             3911..4279
                     /locus_tag="PK518_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRM48290.1"
                     /translation="MSAERIPLCASADLLDGGRAVAFDVHYRGQSLRAFAIRYQGGVH
                     AYLNQCTHVAMEMDFKEGHFFDDSGCRLICSTHGALFAPDTGACLGGPARRPLVKVDL
                     SEEGGMVYWHTAPDLQTVAF"
     gene            4284..5324
                     /locus_tag="PK518_04940"
     CDS             4284..5324
                     /locus_tag="PK518_04940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRM48291.1"
                     /translation="MTDPTRTEPTGFDGNQPQPPDLWARTATETEAKKAVSSTPGWER
                     ELLEKLVLGTLAEQRAARRWRNFWRFGWLALITLIIWAAWQRDLSSTSKSTPHTAVVD
                     VKGEIAAGAEASAEFVVAAMKSAFEDPGSQAVVLLINSPGGSPVQAGMINDEITRLKA
                     KHNKPLYAVVEETCASAAYYIAAAADEIYVDKASIVGSIGVLMDGFGFTGTMEKLGVE
                     RRLLTAGENKGFLDPFSPQSATQRGYAQQMLDQIHQQFIEAVKEGRGERLKATPETFS
                     GLFWTGQQAVDMGLADQYGTLDYVAREVVKAEDVIDYTRRDNVAERLARKFGATFGET
                     VLRGMRAAPALR"
     gene            5500..8187
                     /locus_tag="PK518_04945"
     CDS             5500..8187
                     /locus_tag="PK518_04945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM48292.1"
                     /translation="MNTATAMRALTLSLSPAGHLSCLPDPDAAALPEGLHEPIAAAFA
                     AGAGPGLLQLGGACVTAALPPVWAYWRDLATRYFTALCTAQQGDAPDLPRVPAPDATA
                     LQALVLNAPPMTGAEYLSADALAALWGEIEAALHAACAAAGLPLGEYLKAQYPAWNLV
                     GRVHFNLAENRKDPEAPFAFLATYTTRLSAHGKAQHVPLAQALKEFSGTRQKAQLLSL
                     LLPVQCAAADCAWIAEMVQAGEIYHPLRWEVADALRFLQDLPRMEAAGIVVRMPAQWR
                     SGRPARPMVRAALGSQIAGGVGLDAMLDFRMDVSLDGEPLTPDEVRKLLGSADGLQWI
                     RGQWVQVDARRLSGLLERFRQLEQEAEGGMPLAQAMRLLSGATLGQDAPDPDWSAISA
                     GPWLAEALRGLRDPAALAAIDPGPLLHAQLRPYQQEGLRWLHFLTRLGLGACLADDMG
                     LGKTIQVLALLLVLKKTAPHAGPSLLVAPASLLANWQQEAERFAPSLRCLLAHPSAMA
                     RAELQALDADALAAHDLVITTYGQLSRTPALAAQRWRLAVIDEAQAIKNPAAQQTRAV
                     KKLQAGTRIALTGTPVENRLSDLWSIFDFTHPGLLGSERVFAHFAKRLGETGQYAPLR
                     SLVAPYILRRLKSDKRIIADLPDKTEVSAWCSLSAVQVTHYQQAVKALEQALEKSEGM
                     ERKGLVLSFLMRLKQICNHPSQWLGDGAWAPEASGKFARLREIAETVAARQEKMLVFT
                     QFQETTAPLAAFLGGVFGREGLVLHGGTPVARRRELVKRFQQDEQLPFFVLSIKAGGS
                     GLNLTAASHVVHFDRWWNPAVENQATDRAYRIGQHRNVLVHKFITRGTIEERIDALIQ
                     AKQQLVQDVVEGGGEINLTEMSDAALMDLVKLDINAAGV"
     gene            8193..9128
                     /locus_tag="PK518_04950"
     CDS             8193..9128
                     /locus_tag="PK518_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48293.1"
                     /translation="MGWDYKPYVSVAARRAQADKAAKKAAKAGHAWSPVTVQGRSIAK
                     TFWGKAWCDNLEAYSDFENRLPRGRTYVRNGSVVDLQIQPGKITARVTGSELYQIVID
                     ITALPKAQWQALVQDCTGSIATLVELLQGKLSQAVMERMCKPQTGMFPAPRQIKMACS
                     CPDWAGMCKHVAATLYGVGTRLDEEPQLLFTLRQVDAGDLLTAQAAVSPARAKKPAKA
                     RMLADATLADVFGLDMAVADAPAEATTDKPMSAKRAKKAVVEKAAAPAARKAAVKKPA
                     AKKAVAKKAALPAAKKAVAKKAATGKPAAKKVSAL"
     gene            complement(9168..9950)
                     /locus_tag="PK518_04955"
     CDS             complement(9168..9950)
                     /locus_tag="PK518_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM48294.1"
                     /translation="MSAPKIGTLYLVPAPLDFGCDAEVALTEVLPDGTLRQAAQLTHW
                     ICENAKSCRAYLKRIADLHPLALPLQQQQITELPREVHKKGDHGTKGSASFDARALLA
                     PLLAGQDMGLVSEAGMPAVADPGSSVVRAAHELGARVVPLVGPVSLLLALAASGLNGQ
                     NFAFVGYLPQDAAERAQRLRQLEALALKSGQAQLFIETPYRNAALWQALLQHLQPGTR
                     LATAAGLTLAQAQVQSRSVAQWRALNPAPPDKHLPVVFALGQ"
     gene            complement(9947..10546)
                     /locus_tag="PK518_04960"
     CDS             complement(9947..10546)
                     /locus_tag="PK518_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRM48295.1"
                     /translation="MLQSPPPPCNLVLGSTSRYRRDLLGRLGLPFEVAAPAVDETPLP
                     EESPHALALRLALAKARAVAALRPEAIVIGSDQVADLGGQPLGKPGNHERATAQLRRM
                     SGQTVIFQTAVAVVCQAMGFEQVDLAPVRVRFRSLDDAEIERYLRAEQPYDCAGSAKS
                     EGLGISLLDAIDSDDPTALIGLPLIRTCRMLRAAGLVLP"
     gene            10573..11127
                     /locus_tag="PK518_04965"
     CDS             10573..11127
                     /locus_tag="PK518_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRM48296.1"
                     /translation="MTKEHSPDRLDVKAFAKAGGHLSGHDTLLKYKRLAEEGKGLHPD
                     LRVDWMADGELRTTHGMDGQVWLRLKASASLPLICQRCLHPVDVPLEVEREFRFVADE
                     ATAEALDSESEEDLLVLSREFNLHELIEDELIMVLPVVPRHKRCPSAVKLASSDEDFE
                     QANAQKPNPFAALAGLHKDKPPAE"
     gene            11259..11441
                     /gene="rpmF"
                     /locus_tag="PK518_04970"
     CDS             11259..11441
                     /gene="rpmF"
                     /locus_tag="PK518_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRM48297.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNALTVPGIAVEPTTGETHLRHHISP
                     NGFYRGRQVLKNKSEA"
     gene            11553..12584
                     /gene="plsX"
                     /locus_tag="PK518_04975"
     CDS             11553..12584
                     /gene="plsX"
                     /locus_tag="PK518_04975"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRM48298.1"
                     /translation="MTTLAVDCMGGDHGPGVTLVACRQFLDKHPEARLLLVGQPASLQ
                     AFTHERASVVAASEVVAMDDPVEVALRRKKDSSMRVAIQQVKDGAAQAAVSAGNTGAL
                     MAIARYLLKTLDGIDRPAIATQMPNAQGGATTVLDLGANVDCTAEHLLQFAVMGSALV
                     SVLQEREEPLVGLLNIGEEVIKGSEEIKRAGELLRSAAGAGDLNFHGNVEGNDIFKGT
                     VDIVVCDGFVGNVALKASEGVAAMIIGGLKQEFKRNTFTKLAAVVAYPVLSALMRRMD
                     YRRYNGAALLGLRGLVFKSHGSADVLAFEQALNRAYDAARNNLLDRVRARVAHAAPLL
                     ATADQPRAS"
     gene            12605..13585
                     /locus_tag="PK518_04980"
     CDS             12605..13585
                     /locus_tag="PK518_04980"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRM48299.1"
                     /translation="MRRYSRIAGTGSYLPPRRVTNDDLVAQLAGQGIETSDEWIVERT
                     GIHARHFAERDVSSSDLALQASLRALEAAGRQAQDIDLIIVATSTPDMVFPSTAAILQ
                     HKLGITNGCPVFDVQAVCSGFVYALTVADAMIRSGAANRVLVVGSEVFSRILDFNDRT
                     TCVLFGDGAGAVVLEASDQPGILASQLHADGSHVGILCVPGNVYGGEVMGDPLLKMDG
                     QAVFKLAVGVLEKAARATLDKAGLTEADIDWLIPHQANIRIMQSTARKLKLPMDKVVV
                     TVAEHGNTSAASIPLALDHAVRAGQVKPGQTVLLEGVGGGFTWGAVLLKM"
     gene            13637..>14274
                     /locus_tag="PK518_04985"
     CDS             13637..>14274
                     /locus_tag="PK518_04985"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRM48300.1"
                     /translation="MAQSFAFVFPGQGSQAVGMLDAWGEHPVVAETLREASDALGEDV
                     GALIHAGPKEALALTTNTQPVMLVAGVAAWRVWRAEGGALPAFVAGHSLGEYSALVAS
                     GVLTLSQAAPLVRLRAAAMQEAVPVGAGAMAAILGMDAAKVIAGCAEATAGFGAGSPE
                     VVEAVNFNDPAQTVIAGSKAGVDKACELLKAAGAKRALPLPVSAPFHSSLMKP"
     assembly_gap    14275..14374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14375..14745
                     /locus_tag="PK518_04990"
     CDS             <14375..14745
                     /locus_tag="PK518_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HRM48301.1"
                     /translation="RALPLPVSAPFHSSLMKPAAEKLRVALAALELAAPQIPVLNNVD
                     VAVQQDADAIRDALYRQAFGAVRWVECVQALKARGVTHVIECGPGKVLAGMTKRIDPE
                     LVGAPLYDPATLAEIKELLA"
     gene            14742..15482
                     /gene="fabG"
                     /locus_tag="PK518_04995"
     CDS             14742..15482
                     /gene="fabG"
                     /locus_tag="PK518_04995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRM48302.1"
                     /translation="MSESTQPQIALVTGATRGIGAAIATELAARGLRVIGTATSDDGA
                     ARISQALGPQGGKGVKLNVTDGAAVDALVDAIVKEYGGLHVLVNNAGITRDTLAMRMK
                     DEDWDAVLDTNLKAVFRVSRAVIRPMMKQRFGRIISITSVVGASGNPGQANYAAAKAG
                     VAGMTRALARELGSRGITVNCVAPGFIATDMTADLPEEQKKALKAQIAMGDLGQPSDI
                     AHAVAYLASSGAGYVTGQELHVNGGMYM"
     gene            15587..15826
                     /gene="acpP"
                     /locus_tag="PK518_05000"
     CDS             15587..15826
                     /gene="acpP"
                     /locus_tag="PK518_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM48303.1"
                     /translation="MSDIEARVKKIIAEQLGVEESQVTNEKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEDAEKITTVQNAIDYANTHQKA"
     gene            15885..17129
                     /gene="fabF"
                     /locus_tag="PK518_05005"
     CDS             15885..17129
                     /gene="fabF"
                     /locus_tag="PK518_05005"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRM48304.1"
                     /translation="MSRRRVVVTGLGCVSPVGNTVQDAWANILAGKSGIDLITKFDTS
                     NFACKIAGEVKGFDLESYISAKDARTMDSFIHFGIAAAQEAVRDAGLPTGEALDEELA
                     TRIGCIIGSGIGGLPLIENTHAELTNRGPRRITPFFVPASIINMVAGHVSMRFGFKGP
                     NLSVVTACTTGLHCIGEAARKIEYGDADVIVAGGAEATVSPLGVGGFAAMRALSTRND
                     DPQTASRPWDKGRDGFVLGEGAGVLVLEEYEHAKARGARIYAEVTGYGMSADAGHMTA
                     PNMDGPRRAMQAALRNAGLNADQVDYLNAHGTSTPLGDLNETNAIKAALGDHARKMVV
                     SSTKSMTGHLLGGAGGIESVFTVLALHEQKVPPTINIFEQDPECDLDYCANTARDMKL
                     NVAVKNNFGFGGTNGTLVFQRV"
     gene            17215..17712
                     /locus_tag="PK518_05010"
     CDS             17215..17712
                     /locus_tag="PK518_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48305.1"
                     /translation="MRYPHGRSAVVGWLLLAVAGCGLTSLLGWLVLGTAQADLAIKAA
                     SGLGLWLVCCAVAWRWWRRMPVGQLTWDGGQWVLDSVRPGQAQAVQGSPQVHLDLQWG
                     MLLSVRPPQGRGNWLWLERRSNAVQWLALRRAVYSPARSQAPDLGQTTGSTPSQRNDG
                     APTQT"
     gene            17709..18344
                     /gene="rpoE"
                     /locus_tag="PK518_05015"
     CDS             17709..18344
                     /gene="rpoE"
                     /locus_tag="PK518_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HRM48306.1"
                     /translation="MTAPSPASQEPADSDLRLVERTVAGDQRAYELLVIKYQRRIERL
                     VGRMVRDVDLIPDITQETFLRAYRALHQFRGEAQFYTWLYRIAVNTAKKALLDIKRDP
                     VITESSLRGGGDEDDETSLLDRELTSDETPETVLAAQEVAAAVNAAMEALPEDLRQAV
                     TLREIEGLSYEEISVAMSCPIGTVRSRIFRAREAISARVKPLLEKQTGKRW"
     gene            18384..19019
                     /locus_tag="PK518_05020"
     CDS             18384..19019
                     /locus_tag="PK518_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HRM48307.1"
                     /translation="MNDKDITREHLSALADGELQGEEFARAVAYAGTAEGQSTWRMYH
                     LIGDTLRSPSLAHVTHAADPALVGRLREQLAQEPRPDGVNPAAVATHAAPQGQAEAAN
                     ASVFRWKLAAGFASIAAVVAVGWNGYLGQSGSAAPQAARLAVVQQPAAEDWPPPFVAT
                     AVEPREQQPPVMIRDPRLDELLAAHRQYGNTTALQMPAGFLRNATFEAPRR"
     gene            19094..20572
                     /locus_tag="PK518_05025"
     CDS             19094..20572
                     /locus_tag="PK518_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRM48308.1"
                     /translation="MMSSMDWKRRLQSGSLALMVAVVGSAGLLVSPAPVHAQSAPPLR
                     GLPDFTELVEQVGPSVVNIRTTEKVSARPSMNGMDEEMLEFFKRFGIPMPKIPRQQGP
                     QRQQPEEQPRGVGSGFILSPDGYVMTNAHVVEGADEVLVTLTDKREFKARIVGADKRT
                     DVAVVKIDATGLPAVRVGDVSRLKAGEWVMAIGSPFGLENTVTAGIVSAKQRDTGDYL
                     PFIQTDVAINPGNSGGPLINMRGEVVGINSQIYSRSGGFMGISFAIPMDEAMRVSEQL
                     RTSGRVSRGRIGVQIESVSKDVAESIGLGKPQGALVRGVEVGSPAEKAGIEAGDIITR
                     YEGKAVDKVADLPRLVGNTKPGTKASITVFRRGAMRDLSITIAEIEPDEKVAAKSADA
                     EGKAKPSTAAQQLGLVVAELTPAQAKELKIKGGVRVVSADDAAARAGLRADDVIVALA
                     NTEVRSLKDFEAALAKVDKSKPINVLLRRGEWAQYSLIRPAR"
     gene            21015..>22356
                     /gene="lepA"
                     /locus_tag="PK518_05030"
     CDS             21015..>22356
                     /gene="lepA"
                     /locus_tag="PK518_05030"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRM48309.1"
                     /translation="MNHIRNFSIIAHIDHGKSTLADRIIQRCGGLAEREMEAQVLDSM
                     DIEKERGITIKAQTAALSYKARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVLPVLNKMDLPQADPDNAKAEIEDVIGIDAGEAIA
                     ISAKTGMGIDDVLEQIVAKVPAPRGKPEAPLRAMIIDSWFDSYVGVVMLVRIVDGELK
                     KGERFKMMATGAAYEANQLGVFTPANVQRDALKAGEVGFIIAGIKELKAAKVGDTITL
                     EKKLPNNLGPATEALPGFKEIQPQVFAGLYPTEANQYDQLRDALEKLQLNDASLRFEP
                     EVSQALGFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPSVVYEVVKGDGELIQVEN
                     PSKMPEQGRIQEVREPIVTVHLYMPQDYVGPVMTLANQKRGVQMNMAYHGRQVML"
BASE COUNT         3781 a         7208 c         7535 g         3732 t
ORIGIN      
        1 gggcgtgggc gccgcggccg agcgctgctg gccctgaccg cgtgccggag cttcggggcg
       61 cggctcggcc accggggcgg gggcgtcggg cagcacgccc ttgatcaccg gctgctgctt
      121 gttggtcggc tcttgcgagc ggcgcgtgat gccggtctgc tcctcgggca catcggccag
      181 ctggtagctg gcctccaggc cgtccaggcg cgggtcgtcg tgcttcaggc gctcaagctt
      241 gtagtgcggc gtggccaggc tcttgttcgg gatgagcatg atggccacgc gcttgcgcag
      301 ctcgatcttg gcgatctcgt tgcgcttttc gttcagcagg aagctggcca cctccaccgg
      361 cacctggcag tgcacggcgg cggtgttgtc cttcatggac tcctcctgga tcatgcgcag
      421 catctgcagc gcaaagccct cggtgtcgcg cacatggccg gtgccgtcgc acagcgggca
      481 gcggatatgg gcgccctcgg acagcgaggg cttcaggcgc tggcggctca tctccatcag
      541 gccgaacttg gaaatcgtgc cgaactgcac gcgcgcgcga tcctggcgca gggcgtcgcg
      601 caggcggttc tcgacctcgc ggcggttctt cgactcttcc atgtcgatga agtcgatgac
      661 gatcaggccg cccaggtcgc gcaggcgcat ctggcgcgcc acctcgtcgg cggcctccag
      721 gttggtgcgg gtggcggtct cctcgatgtc gccgcccttg atggcgcgcg ccgagttcac
      781 gtccacgctg accagcgcct cggtgtggtc gatgacgatg gcaccgccag acggcagggt
      841 gaccgtgcgg gcgtaggccg attcgatctg gtgctcgatc tggaagcgcg agaacagcgg
      901 cgcatcatcg cggtagcgct tgacgcgcgc ggcctgctcg ggcatgacat gcgccatgaa
      961 ctgctgggcc tgctcgtaga tgtcgtcggt gtcgatcagg atgtcgccga tgtcggcgtt
     1021 gaagtagtcg cggatggcgc ggatcaccag gctcgactcc tggtagatca ggaaggcgcc
     1081 cttgccggcc ttggccgcgc cgtcgatggc gctccacagc ttcagcaggt agttcaggtc
     1141 ccattgcagc tcgggggcgc tgcggccaat accggcggtg cgcgcgatga tggacatgcc
     1201 cttggggtat tcgagctggt ccatggcctc tttgagctcg gcccggtcct cgccctcgat
     1261 gcggcggctg acgccgccgc cgcgcgggtt gttgggcatc agcaccacgt agcggccggc
     1321 caggctgatg aaggtggtca gcgccgcgcc cttgttgccg cgctcttcct tctccacctg
     1381 caccagcagt tcctggcctt ccttgatcac atcgttgatg cgcgcctgag agggcgagac
     1441 gccctcggcg aaatactggc gcgagatttc cttgaagggc agaaagccgt ggcgatcctc
     1501 gccatagtcg acgaagcagg cctccagcga cggctcgacg cgcgtgacca ccgccttgta
     1561 gatgttgccc ttgcgctgct cgcgcccttc gacctcgatc tcgtaatcca gcagcttctg
     1621 gccgtcgacg atggccaggc ggcgttcttc gggctgcgtg gcgttgatga gcatccgttt
     1681 catgatgcgt gtcctttatg caatgcgttg ttcttgtgcg ggcagtgcac ggcgctgccc
     1741 gcgtctcgta tccagacaaa caccctcggc agaggggcgt caatgcctgg actggcgcat
     1801 cgaaacggaa gcgagggaag gggccgggtc gcaccacagc cgcgcagcgg gtcagctgcg
     1861 cagcggtgga ggagggtggg cgtggatggg agcgagccgg attgcaggct gttcagctat
     1921 caaaattaga gctgttcgcg ctttccggat gggcgttgga ggcgattttt gtgccaacgg
     1981 caggcgctgg cgcacctgcc atagggacac aatccaagcc agtacaaccc acatgatgtc
     2041 gctttgatcc gccgaccgcc gcgctctggt ggaggcggtg tcggcttgta tttccgtgtt
     2101 tctgtgattc gatgcgtcag cctctggtgc agggtgcggc gctacaaacc gcgcactacg
     2161 cgcctgggca ttcaccagac cgcgcgtcga gccgggccgt gtccactaaa ctccagacac
     2221 atcaaccgct tacacatccg tcgcgcaggt gaaaccgatt atagagggca aacccagccc
     2281 gacccccgcg gcccgcatcg tcgaggtcga tgccgagtcc acaggccagc gcctggacaa
     2341 ctttttgata cgccacctca agggcgtgcc caagacccat gtctaccgca tcatccgcag
     2401 cggcgaggtg cgcctgaaca agggccgcac cagcgccgac gcgcgcgtgg ccgagggcga
     2461 cctggtgcgc ctgccgccgg tgcgcgtgtc cgagaagatc gccgagaagg ccgagcgccc
     2521 ggcgcccgcg cgtgaattcc ccatcctgct cgaggacgag cagctgatcg ccctggacaa
     2581 gcccgccggc gtggccgtgc atggcggctc gggcgtttcc tttggcgtca tcgagcagct
     2641 gcgccaggcc aggccgcagg cgcgctttct ggagctggtg caccggctcg atcgcgagac
     2701 ctcgggcatc ctgctggtgg ccaagaagcg ctcggcactc acgcacctgc aggaccagtt
     2761 ccgcgggcgc gagaccggca agacctacct ggcgctggtg actggcagct ggccggcgaa
     2821 ccacaaggtt attgaccagc cgctgcacaa atacctgcag gagggcggcg agcgccgcgt
     2881 gcgcgtgacc acccaggacg acccggacgg catgcgctcc atcaccctgg tcaaggtacg
     2941 cgagcaactg gcgccgcgcc ccatggccgg cctgccggcc atgagcctgc tggaggtgac
     3001 catcaagacc gggcgcacgc accagatccg cgtgcacctg gcgtcccagg gccaccctat
     3061 cgccggcgac gacaagtacg gcgacttcga cctgaaccgc cgtttgcaga agaacggcct
     3121 gaaacgcatg ttcctgcacg cctggcgtct gcagttcgac cacccgggca gcggcgagcg
     3181 cctggagctg cgtgccgagc tgccgccgga tctggcggac tttcttgcca ctgagcccac
     3241 ccatgcctga actccgcccg cgccgcttcg acctgattgc cttcgactgg gacggcacgc
     3301 tgttcgactc caccgccatc atcgtgcgca gcattcagga cgccgtgcgc gacgtcggcg
     3361 gcgccgtgcc cagtgatgag gccgccgcct gggtgattgg catggcgctg gagccggcgc
     3421 tggcgcatgc cgcgcccgac gtgccgcgcg agcactatcc gcagctggcc aaccgctacc
     3481 gttaccacta cctcaaacgc caggacgatg tgaccctgtt tgccggcgtg ctgcccatgc
     3541 tgcatgccct gcgtgcgcgc ggccacctgc tggccgtggc cacgggcaag agccgccgcg
     3601 gcctggacca ggcgttgcag ctggtgcagc tgcgcggcat gttcgacggc tcgcgcacgg
     3661 ccgacgagac ggccggcaag ccgcacccgc tgatgctgca ggagctgatg gccgagttcg
     3721 acgtggcgcc cgggcgcctg ctcatgattg gcgacaccac gcatgacctg cagatggccc
     3781 gcgccgccgg ctgcgccagc gtgggcgtgg gctatggcgc ccatgacgtg gccgagttcg
     3841 ccgccctggg cccgctgtgt gtggtgccat cggtcgccgc gctgcacggc tggctgcagg
     3901 agcacgcctg atgagcgccg agcgcatccc cctgtgcgcc agcgccgatc tgctggacgg
     3961 cgggcgcgcc gtggccttcg atgtgcacta tcgcggccag agcctgcgcg cctttgccat
     4021 ccgctaccag ggtggcgtgc atgcctatct gaaccaatgc acccatgtgg ccatggaaat
     4081 ggatttcaag gagggccatt ttttcgacga cagtggctgc cggctgatct gctccaccca
     4141 cggagcgttg tttgcgcccg acaccggcgc ctgcctgggc ggcccggcac gccggcccct
     4201 ggtcaaggtg gacttgagcg aagagggcgg catggtgtac tggcatactg cccccgatct
     4261 gcaaaccgta gcgttctgac accatgaccg accccacccg caccgagccc accggttttg
     4321 atggaaatca gccccaaccg cccgacctgt gggcgcgaac agctactgaa acagaagcaa
     4381 aaaaggcggt ctccagcacg ccgggctggg agcgcgagct gctcgaaaaa ctcgtcctgg
     4441 gcaccctggc cgagcagcgc gccgccaggc gctggcgcaa cttctggcgc tttggctggc
     4501 tggcgctgat caccttgatc atctgggccg cctggcagcg cgacctgtcc agcaccagca
     4561 agagcacgcc gcacaccgcg gtggtggatg tcaagggcga gatcgccgcc ggcgccgagg
     4621 ccagcgccga gttcgtcgtc gccgccatga agagcgcctt cgaggacccg ggctcgcagg
     4681 ccgtggtgct gctcatcaac tcgcccggcg gcagcccggt gcaggcgggc atgatcaacg
     4741 acgagatcac ccgcctgaag gccaagcaca acaagcccct gtacgccgtg gtcgaggaga
     4801 cctgcgcctc ggccgcctac tacatcgccg cggcggccga cgagatctac gtggacaagg
     4861 ccagcatcgt cggcagcata ggcgtgctga tggacggctt tggcttcacc ggcaccatgg
     4921 aaaagctggg cgtcgagcgc cgcctgctca ccgccggtga gaacaagggc tttctcgacc
     4981 ccttcagccc gcagtcggcc acgcagcgcg gctatgcgca gcagatgctc gatcagatcc
     5041 accagcagtt catcgaggcc gtgaaagagg gccggggcga gcgcctgaag gccacgcccg
     5101 agaccttcag cggcctgttc tggaccggcc agcaggccgt ggacatgggc ctggccgacc
     5161 agtacggcac cctggactac gtggcgcgcg aggtcgtcaa ggccgaggac gtgatcgact
     5221 acacgcgccg cgacaacgtc gccgagcgcc tggcgcgtaa attcggcgcc acctttggcg
     5281 aaaccgtgct gcgcggcatg cgggcggcgc cggcgctgcg ttgagtgcat gaacacagcg
     5341 ccgcatgccg gcctggagct ggagcgccat gtcgtaccgc agcggctgca ctctgggcgg
     5401 gtttgtctaa ctataaaaac aatagctgtt ggcgcttgat gggtgctggt tttaattgat
     5461 tttcatcata tataacttgc ctgccgcgcg catttgtgca tgaacaccgc aaccgccatg
     5521 cgtgcgctga ccctgtccct gtctcccgcg ggccatctgt catgcttgcc cgatcctgac
     5581 gcagcggccc tgcccgaggg cttgcacgag cccattgccg ctgcatttgc cgcgggcgcg
     5641 gggccaggtc tgctgcagct gggcggcgcc tgcgtcacgg cggccctgcc gccggtctgg
     5701 gcctattggc gggatctggc cacgcgctac ttcacggccc tgtgcaccgc gcagcagggc
     5761 gatgcgccgg acctgccccg cgtgcccgcg cccgatgcga cggccctgca ggcgctggtg
     5821 ctgaacgcgc cgcccatgac cggcgccgaa tacctgagcg cggacgccct ggcggcactg
     5881 tggggcgaga tcgaggcagc gttgcacgcg gcctgcgcgg cggccgggct gcccctgggc
     5941 gaatacctca aggcccagta cccggcctgg aacctggtcg gccgcgtgca cttcaacctg
     6001 gcggaaaacc gcaaggatcc cgaggccccg tttgcctttt tggccaccta caccacgcgc
     6061 ctgtcggccc atggcaaggc ccagcatgtg ccgctggcgc aggcgctgaa agaattttcc
     6121 ggcacgcgcc agaaggcgca gctgctgtcg ctgctgctgc cggtgcagtg cgccgccgca
     6181 gactgcgcat ggatcgccga gatggtgcag gccggcgaga tctaccaccc gctgcgctgg
     6241 gaggtggcgg acgcgctgcg ctttctgcag gatctgccgc gcatggaggc cgccggcatc
     6301 gtggtgcgca tgccggccca gtggcgcagc ggccgccccg cgcggcccat ggtgcgcgcc
     6361 gcgctgggca gccagattgc gggcggcgtg ggtctggacg ctatgctcga cttccgcatg
     6421 gacgtgagcc tggacggcga gccgctcacg cccgatgagg tgcgcaaact gctgggcagc
     6481 gccgatggcc tgcagtggat acgcggccag tgggtgcagg tggacgcgcg gcgcctgtcc
     6541 ggcctgctgg agcgctttcg ccagctggag caggaggccg agggcggcat gccgctggcg
     6601 caggccatgc gcctgctctc gggcgccacg cttggccagg acgcacccga ccccgactgg
     6661 agcgccatca gcgccggccc ctggctggcc gaggcgctgc gcggcctgcg cgatcctgct
     6721 gccctggccg ccatcgatcc cggcccgctg ctgcatgccc agctgcgtcc ctatcagcag
     6781 gagggcctgc gctggctgca ctttttgacg cgcctggggc tgggcgcctg cctggccgac
     6841 gacatgggcc tgggcaagac catacaggtg ttggcgctct tgctggtgct gaagaagacc
     6901 gcgccgcatg ccggccccag cctgctggtg gcgccggcct cgctgctggc caactggcag
     6961 caggaggccg agcgcttcgc ccccagcctg cgctgcctgc tggcccatcc gtcggccatg
     7021 gcgcgcgccg agctgcaggc gctcgatgcc gatgcgctgg cggctcacga cctggtcatt
     7081 accacctacg gccagctgtc acgcaccccg gccctggccg cgcagcgctg gcgcctggcg
     7141 gtgatcgacg aggcgcaggc catcaagaac ccggcggcgc agcagacccg cgcggtcaag
     7201 aaactgcagg ccggcacgcg catcgccctc accggcacgc cggtggagaa ccgcctgtcg
     7261 gatctgtggt cgatcttcga cttcacccat cccggcctgc tgggcagcga gcgcgtgttt
     7321 gcccactttg ccaagcgcct gggcgaaacc ggccagtacg cgccgctgcg cagcctggtg
     7381 gcgccctaca tactgcggcg cctgaagagc gacaagcgga tcattgccga cctgcccgac
     7441 aagaccgagg tctctgcctg gtgcagcctg agcgccgtgc aggtcacgca ttaccagcag
     7501 gccgtcaagg ccctggagca ggcgctggag aagtccgagg gcatggagcg caagggcctg
     7561 gtgctctcgt tcttgatgcg attgaagcaa atctgcaacc atccctcgca atggctgggc
     7621 gacggcgcct gggcgcccga ggccagtggc aagtttgcgc gcctgcgtga gatcgccgag
     7681 accgtggccg cgcggcagga gaagatgctg gtgttcaccc agttccagga aaccacggcg
     7741 ccgctggcgg cgtttctggg cggcgtgttt gggcgcgagg gcctggtgct gcatggcggc
     7801 acgcccgtgg cgcggcggcg cgagctcgtc aagcgcttcc agcaggacga gcagctgccg
     7861 ttcttcgtgc tctccatcaa ggcaggcggc tccgggctga acctgaccgc cgcctcgcat
     7921 gtggtgcact tcgaccgctg gtggaacccg gccgtggaga accaggccac cgaccgcgcc
     7981 taccgcatcg gccagcaccg caacgtgctg gtacacaaat tcattaccag gggcaccata
     8041 gaggagcgca tcgacgcgct gatccaggcc aagcaacagc tggtgcagga tgtggtggag
     8101 ggcggcggcg aaatcaacct caccgaaatg agcgacgccg cgctcatgga tctggtgaaa
     8161 ctcgacatca atgcggcagg ggtgtaacca tcatgggctg ggactacaaa ccgtatgtca
     8221 gcgtggccgc gcgtcgggcg caggcggaca aggcggcgaa aaaggccgcc aaggcggggc
     8281 acgcctggag ccccgtgacg gtgcaggggc gcagcatcgc caagaccttc tggggcaagg
     8341 cctggtgcga caacctggag gcctacagcg actttgaaaa ccgcctgcca cgcggccgca
     8401 cctatgtgcg caatggctcg gtggtggacc tgcagatcca gcccggcaag atcacggcca
     8461 gggtcacggg ttcagagctg taccagatcg tgatcgacat cacggcactg cccaaggcgc
     8521 agtggcaggc gctggtgcag gactgcacgg gctccattgc cacgctggtc gagctgctgc
     8581 agggcaagct gtcgcaggcg gtgatggagc gcatgtgcaa gccgcaaacg ggcatgtttc
     8641 cggcgccgcg ccagatcaag atggcttgct cctgtcccga ctgggcaggc atgtgcaagc
     8701 atgtggcggc cacgctctat ggcgtgggta cgcgtctgga tgaagagccg cagctgctgt
     8761 tcacgctgcg ccaggtggac gcgggcgacc tgctcacggc ccaggccgcc gtgtcccccg
     8821 cccgggcgaa aaagccggcc aaggccagga tgctggccga cgcgacgctg gccgatgtgt
     8881 ttggcctgga tatggccgtg gcggatgcgc cggccgaggc aacaacggat aagcccatgt
     8941 ctgccaagcg cgccaaaaag gccgtggtgg aaaaggccgc ggcgccagca gccaggaagg
     9001 ccgccgtgaa aaagcccgca gccaagaagg ccgttgccaa gaaggctgcg ctgcctgccg
     9061 caaagaaggc tgtagccaag aaggccgcga ccggcaagcc cgcggcgaag aaggtcagcg
     9121 ccttgtagca gtggctggcc gcaccggttc cgccaggtgg ccagacctca ttgccccagg
     9181 gcaaacacca ccggcaggtg cttgtcgggc ggcgccgggt tcagcgcgcg ccattgcgcc
     9241 acgctgcggc tttgcacctg tgcctgggcc agcgtcaggc ctgcggccgt ggccaggcgc
     9301 gtgccgggct gcagatgctg cagcagcgcc tgccacagcg cggcgttgcg gtagggcgtc
     9361 tctatgaaca gttgcgcctg tccgctcttg agcgccaggg cttcgagctg gcgcaggcgc
     9421 tgggcgcgct cggcagcgtc ctgcggcagg tagccgacaa aggcaaagtt ctggccattg
     9481 agtccgctgg ccgccagcgc caggagcagc gacaccggcc ccaccagcgg caccacgcgc
     9541 gcgcccagct cgtgcgcggc gcgcaccacc gagctgcccg ggtcggccac ggcgggcatg
     9601 ccggcctcgc tcaccaggcc catgtcctgc cccgccagca ggggcgcgag cagtgccctg
     9661 gcgtcaaagc tggcgctgcc cttggtgcca tggtcgccct tcttgtgcac ctcgcgcggc
     9721 agctcggtga tctgctgctg ctgcaggggc agggccagcg ggtgcaggtc ggcaatgcgc
     9781 ttcaagtagg cgcggcagct cttggcgttc tcgcagatcc agtgggtgag ctgcgccgcc
     9841 tggcgcagcg tgccgtccgg cagcacctcg gtcagcgcca cttcggcgtc gcagccaaag
     9901 tccagcgggg cgggtaccag gtacagcgtg ccgatcttgg gcgcgctcat ggcagcacca
     9961 gcccggcggc gcgcagcatg cggcaggtgc gtatcagcgg caggccgatc agggccgtgg
    10021 ggtcgtcgct gtctatggcg tctagcaggc tgatgcccaa cccctcgctc ttggcgcttc
    10081 cggcgcagtc gtagggctgc tcggcgcgca gatagcgttc gatctcggcg tcgtccaagg
    10141 agcgaaagcg cacccgcacc ggcgccagat ccacctgctc gaaacccatg gcctggcaga
    10201 ccacggccac ggcggtctgg aagatcacgg tctggccgct catgcggcgc aactgggccg
    10261 tggcgcgctc gtggttgccg ggcttgccca ggggctggcc gccgaggtcg gccacctgat
    10321 ccgagccgat cacgatggcc tcgggccgca gtgccgcaac ggccctggcc ttggccaacg
    10381 ccaggcgcag cgccagggcg tgcggtgact cttcaggcag cggggtttca tcgaccgcgg
    10441 gggcggccac ctcgaagggc aggcccaggc ggcccagcag gtcacgccgg tagcgcgagg
    10501 tcgatcccaa taccaggttg cagggcgggg gaggggattg cagcatgttc cgattctctt
    10561 acactgcctg ccatgaccaa ggaacattct cccgaccggc tggacgtcaa ggcctttgcg
    10621 aaggccggcg gccacctgtc cggtcatgac accctgctca agtacaagcg cctggccgaa
    10681 gagggcaagg gcctgcatcc cgacctgcgc gtggactgga tggccgacgg cgagttgcgc
    10741 accacccatg gcatggacgg ccaggtctgg ctgcgcctga aggccagcgc cagcctgccc
    10801 ctgatctgcc agcgctgcct gcacccggtg gacgtgccgc tggaagtgga gcgcgagttc
    10861 cgtttcgtgg ccgacgaggc cacggccgag gctctggaca gcgagagtga agaagacctg
    10921 ctggtgctga gccgtgagtt caacctgcac gaactcatag aagacgaact catcatggtc
    10981 ttgcccgtgg tgccgcggca caagcgatgc ccgtcggcgg tgaagctggc atcgagcgac
    11041 gaggacttcg agcaggccaa tgcgcaaaag cccaatccct tcgcggccct ggccggtttg
    11101 cacaaggaca agccgccagc ggagtgactc cagtctggcg caaggcaggg tacaaacgca
    11161 tttggcatat aatggcaggc ttcgcgcgaa accccctcgt gtcttcaatg ggctgatcgc
    11221 gttttttacc aacccctgca agaccctagg agccaatcat ggctgttcag caaaacaaga
    11281 agtccccttc caagcgcggc atgcaccgtt cgcacaacgc cctgacggtg cccggcattg
    11341 ccgtggaacc caccaccggc gaaacccatc tgcgtcacca catcagcccc aacggtttct
    11401 atcgtggtcg ccaggtgctg aagaacaagt ctgaagcctg atagcagact gcgcaatctc
    11461 gggcccgttg ctacaattta agtagcgcgg gcctttgtta ttttggctcg cgccaagatt
    11521 ttcagaccac ctgacggatt gattctccgc cgatgaccac tctggctgtt gattgcatgg
    11581 ggggcgacca cggccccggt gtcacgcttg tcgcgtgtcg ccagtttctg gacaaacacc
    11641 ccgaggcgcg cctgctgctg gtcgggcaac ccgcaagcct gcaggcgttc acgcatgagc
    11701 gcgcctcggt ggtcgcggcc tcggaggtcg tggccatgga tgatccggtc gaggtggccc
    11761 tgcgccgcaa gaaggattcg tcgatgcgcg tggccatcca gcaggtcaag gacggtgcgg
    11821 cgcaggctgc ggtgtcggct ggcaacaccg gcgcgctgat ggccattgca cgctacctgc
    11881 tcaagaccct ggacggcatt gaccgcccgg ccattgccac gcagatgccc aatgcccagg
    11941 gcggcgccac cacggtgctg gatctgggcg ctaatgtcga ttgcacggcc gagcatttgc
    12001 tgcagttcgc cgtcatgggc tcggccctgg tgtcggtgct gcaggaacgc gaggagccgc
    12061 tggtcggcct gctcaatatc ggtgaggaag tcatcaaggg cagcgaggag atcaagcggg
    12121 cgggcgagct gctgcgctcg gcagccggcg ccggtgactt gaactttcac ggcaacgtcg
    12181 aaggcaacga tatcttcaag ggcacggtgg acatcgtggt ttgcgatggc tttgtcggca
    12241 acgtggcgct caaggccagc gaaggcgtgg ctgcgatgat cattggcggg ctcaagcagg
    12301 agttcaagcg caataccttc accaagctgg cggcggtggt ggcctatccg gtgctgtccg
    12361 ccctgatgcg gcgcatggat taccgccgtt acaacggcgc cgcattgctg ggcctgcgcg
    12421 gcctggtgtt caagagccac ggctcggccg acgtgctcgc attcgagcag gctttgaacc
    12481 gggcgtatga tgcggcccgc aacaacctgc tagaccgcgt ccgtgcgcgg gttgcgcatg
    12541 cggcgcccct gctggctacg gcagaccagc cgcgcgcatc ctgaacggtg gcggccccct
    12601 attgatgaga cgctattccc gcattgccgg caccggcagc tatctgccgc cgcgccgcgt
    12661 gaccaacgat gacctggtgg cccagctggc cgggcagggc atagagacct ctgatgaatg
    12721 gatcgtggag cgtacgggca tccatgcgcg ccatttcgcc gaacgcgacg tgagcagcag
    12781 cgacctggcg ctgcaggcat cgctgcgcgc actggaggcc gccggccgcc aggcgcagga
    12841 catcgatctg atcatcgtcg ccacgtccac gccggacatg gttttcccct ccaccgcagc
    12901 catcctgcag cacaagctgg gcattaccaa tggttgcccg gtgttcgatg tgcaggccgt
    12961 atgcagcggc tttgtctacg cgctgacggt ggcggacgcg atgatccggt ccggtgccgc
    13021 caaccgcgtg ctggtcgtgg gctccgaggt gttcagccgc atccttgatt tcaacgaccg
    13081 caccacctgc gtgctgtttg gcgacggcgc cggcgccgtg gtgctggagg cttccgacca
    13141 gccgggcatc ctggccagcc agttgcacgc cgacggcagc catgtgggca ttctgtgtgt
    13201 acccggcaat gtctatggcg gcgaggtaat gggcgatccg ctgctcaaga tggatggtca
    13261 ggccgtgttc aagctggccg tgggcgtgct tgaaaaggcc gcccgcgcca cgctggacaa
    13321 ggctggcctg acggaggccg acatcgattg gctgattccg caccaggcga atatccgcat
    13381 catgcaaagc acagcgcgca agttgaagct gcccatggac aaagttgtcg tcacggtggc
    13441 tgagcatggc aatacctcgg cggcctcgat cccgctggcg ctggatcacg ccgtgcgcgc
    13501 gggccaggtc aagccggggc agacggtgct gctcgagggc gtcggcggtg gtttcacctg
    13561 gggtgcggta ctccttaaaa tgtagctgtt tgcgcttgtt ctatgggcgt tacaggcctt
    13621 ttttactgaa taaaccatgg ctcaatcttt tgcatttgtc tttcccggcc agggctccca
    13681 ggccgtgggc atgctcgacg cctggggcga gcaccctgtg gtggccgaga cgctgcgcga
    13741 ggcctccgac gcattgggcg aggatgtcgg cgcgctgatc cacgccggtc ccaaggaggc
    13801 cctggcgctg accaccaaca cccagccggt gatgctggtg gccggcgtcg ccgcctggcg
    13861 cgtgtggcgt gccgagggcg gcgcgctacc tgcgttcgtg gctgggcatt cgttgggtga
    13921 gtattcggcg ctcgtcgctt cgggcgtgtt gactctgtcg caggccgcgc ccctggtgcg
    13981 cctgcgtgcc gcagccatgc aggaggccgt gcccgtggga gcgggcgcca tggccgccat
    14041 tctgggcatg gatgccgcca aggtcattgc cggctgcgcc gaggccacgg ccggctttgg
    14101 tgccggatcg cccgaggtgg tggaggccgt gaacttcaac gacccggcgc agaccgtgat
    14161 cgccggcagc aaggccggcg tggacaaggc ctgcgagctg ctcaaggcgg caggcgccaa
    14221 gcgtgccctg ccactgcccg tgtcggcgcc gtttcactcc agcctgatga agccnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcgtg ccctgccact gcccgtgtcg
    14401 gcgccgtttc actccagcct gatgaagcca gcagccgaaa aactgcgcgt ggcgcttgcc
    14461 gcgctggaac tggccgcgcc gcagatcccc gttctgaaca acgtggacgt ggcggttcag
    14521 caggatgcag acgccatccg cgatgccctg taccgccagg ccttcggcgc cgtgcgctgg
    14581 gtggagtgcg tgcaggccct caaggctcgc ggcgtcaccc atgtcatcga atgcgggcct
    14641 ggcaaggtgc tcgccggcat gaccaagcgc atagacccgg agctggtcgg cgcccctctg
    14701 tacgacccgg ccacgctggc cgaaatcaag gaattgctgg catgagcgag tccacccaac
    14761 cccagattgc gctggtcacc ggcgccacgc gcggcattgg cgccgccatt gccactgaac
    14821 tggcagcacg cggcttgcgc gtgatcggca cggccacctc cgacgatggc gcggcgcgca
    14881 tcagccaggc cctggggccc caaggcggca agggcgtaaa gctcaacgtg accgacggtg
    14941 ccgccgtgga tgcgctggtg gatgccatcg tgaaggaata tggcggcctg catgtgctgg
    15001 tgaacaatgc cggcatcacg cgcgacaccc tggccatgcg catgaaggat gaggactggg
    15061 acgccgtgct ggacaccaat ctcaaggcgg tgttccgcgt cagccgtgcg gtcatccgcc
    15121 cgatgatgaa gcagcgcttc gggcgcatca tcagcatcac cagcgtggtc ggcgcctcgg
    15181 gcaaccccgg ccaggccaac tacgcggcgg ccaaggccgg cgtggccggc atgacgcgcg
    15241 ccctggcgcg cgaactgggc agccgcggca tcaccgtgaa ctgcgtggcc cccggcttca
    15301 ttgccaccga catgacggcc gacctgcccg aagagcagaa gaaggctctc aaggctcaga
    15361 tcgccatggg tgacctgggc cagcccagcg acattgcaca tgccgtggcc tatctggctt
    15421 ccagcggtgc cggctatgtg acggggcagg aattgcacgt caatggcggc atgtacatgt
    15481 agttgaacca aattaacgcc ggatagtccg tgcggcgagc cagctagggg tctttgcccg
    15541 aagcggctag aatcgcggat tcattcacaa ccccccgagg gaaactatga gcgatatcga
    15601 agcacgtgtc aaaaaaatca ttgccgaaca actcggcgtg gaagagtctc aagtcaccaa
    15661 cgaaaaggcc ttcgtggctg atctgggtgc cgactcgctg gacacggtgg aactggtgat
    15721 ggcactggaa gacgaattcg gcatcgaaat ccctgacgaa gacgcagaga agatcaccac
    15781 ggtgcaaaac gccatcgact acgccaacac gcaccaaaag gcctaagtcg ctgcggcccc
    15841 ggtgttcagc ggggcgcagc actcgatcag caaaggttta acgcatgagc cgtcgtcgcg
    15901 ttgtcgtgac cggcctgggt tgcgtcagtc ccgtgggcaa cacggtgcag gacgcctggg
    15961 ccaatatcct cgccggaaag tccggcatcg acctcatcac caagttcgat acgtcgaact
    16021 ttgcctgcaa gattgccggc gaggtcaagg gttttgacct ggagtcctac atcagcgcca
    16081 aggatgcgcg caccatggac agcttcatcc atttcggcat tgccgcggct caggaggcgg
    16141 tgcgcgacgc cggcctgcct accggggagg cactggatga agaactggcc acgcgcatag
    16201 gctgcatcat cggctcaggg ataggtggcc tgccgctgat cgagaacacc cacgcggagc
    16261 tgaccaatcg tggtccgcgt cgcatcacgc cgttcttcgt gccggcgtcc atcatcaaca
    16321 tggtggcggg ccatgtgtcc atgcgtttcg gcttcaaggg ccccaatctt tccgtggtga
    16381 cggcctgtac gaccggcctg cactgtattg gcgaggcggc gcgcaagatc gagtatggcg
    16441 atgccgacgt gatcgtggcc ggtggcgccg aggccaccgt gtcgcccctg ggcgtgggcg
    16501 gttttgctgc catgcgcgcc ctgtccacgc gcaacgacga tccgcagacg gcgtcccgcc
    16561 cctgggacaa gggccgcgac ggcttcgtgc tcggcgaggg cgcgggcgtg ctcgtgctcg
    16621 aggaatacga gcatgccaag gcacgtggcg cgcgcatcta cgccgaggtc accggctatg
    16681 gcatgagcgc cgacgccggc cacatgacgg cacccaacat ggacggcccg cgccgcgcca
    16741 tgcaggcggc gttgcgcaat gccggcctga atgccgacca ggtggactac ctgaatgccc
    16801 atggcacatc cacgccgctg ggcgatctga acgaaaccaa tgccataaag gcagcgctgg
    16861 gcgaccatgc caggaagatg gtggtgagct ccaccaagtc gatgaccggc catctgctcg
    16921 gtggtgccgg cggcatagag agcgtgttca ccgtgctcgc gctgcacgag cagaaggtgc
    16981 cgccgacgat caacatcttt gagcaggatc cggagtgcga cctggattac tgcgccaaca
    17041 ccgcgcgcga catgaagctg aatgtcgcgg tgaagaacaa ctttggcttt ggcggcacca
    17101 acggcacgct ggtattccaa cgcgtctgat tgccgcggac aagcgcttgc ttggcccagg
    17161 aacgatccga tgggacgcgg ttagacaatg cgggccccat cgcatgcgcc cgccgtgcgc
    17221 tatccccatg gccgttcagc ggtggttggc tggctgttgc tggcagttgc cggctgcggc
    17281 ctgaccagtt tgcttggctg gctggtgctc ggtacggcac aggcggacct ggcgatcaag
    17341 gccgcgtcgg gcctgggtct ttggctcgtt tgctgcgctg tcgcctggcg ttggtggcgg
    17401 cgcatgccgg tgggtcaatt gacatgggat ggcggccaat gggtgcttga ctccgtccgg
    17461 cccgggcagg cgcaggctgt gcaaggcagt ccgcaagtcc atctggatct gcaatggggc
    17521 atgctgctct cggtgcgacc gccgcaaggg cggggcaatt ggctgtggct ggagcggcgc
    17581 agcaacgccg ttcaatggct ggcgctgcgg cgtgcggtat attcgccggc ccgctcccag
    17641 gccccagatc tgggccagac aaccggcagc acgccaagcc agcgcaacga cggagcaccc
    17701 acccagacat gaccgcacct tcgcccgcat cccaggagcc cgccgacagc gatctgcggc
    17761 tggtcgagcg cacggttgcc ggcgaccagc gtgcctatga gttgctggtg atcaagtacc
    17821 agcgccgcat cgagcgtctg gtggggcgca tggtgcgtga cgtggatctg atcccggaca
    17881 tcacgcagga aacctttttg cgtgcctatc gtgcgctgca ccagttccgc ggcgaggcgc
    17941 agttctacac ctggctttac cgcattgcag tgaacacggc caagaaggcg ctgctggaca
    18001 tcaagcgcga cccggtgatc acggaaagca gcctgcgtgg gggcggggat gaagacgatg
    18061 aaacttccct gctcgatcgt gaactaacca gcgatgaaac ccccgaaacc gtgcttgccg
    18121 cccaggaggt tgcagccgcg gtcaacgccg ccatggaggc gttgcccgag gatttgcgcc
    18181 aggccgtcac actgcgggag atcgagggtt tgagttacga ggaaatttcc gtggccatgt
    18241 cctgccccat aggcaccgtg cgctcgcgga ttttccgggc ccgtgaggcc atatcggcaa
    18301 gggtcaagcc gctgctggag aagcagaccg gcaaacggtg gtagtgaaag aggcgcagat
    18361 tgcgtgcaat ggcaggtgac accatgaacg acaaggacat cacgcgtgag catctgtcgg
    18421 ccctggccga cggcgagttg cagggcgagg agttcgcccg tgccgtggcc tatgccggca
    18481 cggcagaagg ccagtccacc tggcgcatgt atcacctgat cggtgacacc ctgcgcagcc
    18541 cgtcgctggc gcatgtgacg catgcagccg atccggcgct ggtgggccgg ttgcgtgagc
    18601 aactggcgca ggaacctcgc cccgatggcg tgaacccggc cgcggtggcg acccatgctg
    18661 ccccccaggg acaagctgag gcggccaatg cctcggtgtt ccgctggaag cttgccgccg
    18721 gttttgcgtc gattgcggcg gtggtcgccg tgggctggaa tggctacctg gggcagtccg
    18781 gtagcgcagc gccccaggcg gcacggctgg cggtagtcca gcagccggcc gcagaagact
    18841 ggcccccgcc gtttgtggct accgctgtgg agccccggga gcagcagccg ccggtgatga
    18901 tccgcgaccc gcgcctggac gagttgctgg ccgcgcacag gcagtacggc aacaccacgg
    18961 cgttgcagat gccggcgggg ttcttgcgca acgccacctt tgaggcgccc aggcgttgaa
    19021 cccgcccgtt tctttgtgcg cgcaatgcga tgcgcggcgc agccactaca caagcatatg
    19081 gagggaactg ccgatgatgt cgagcatgga ttggaaacgc cggctgcagt ccggttcttt
    19141 ggccctgatg gttgccgtgg ttggctcggc cggcctgctg gtcagcccgg cgccggtgca
    19201 tgcccagtcg gcgccgccct tgcgcggctt gcctgacttc accgaattgg tggaacaggt
    19261 ggggccgtcg gtggtgaaca tccgcaccac ggagaaggtg tctgcccgcc cgtcgatgaa
    19321 tggcatggat gaggagatgc tggaattctt caagcgcttc ggcattccca tgcccaagat
    19381 tccacgccag caggggccgc aacgccagca acccgaggag cagccgcgcg gcgtgggttc
    19441 gggcttcatc ctctcgcccg acggctatgt gatgaccaac gcccatgtgg tcgagggggc
    19501 cgatgaggtg ctggtgacgc tgaccgacaa gcgcgaattc aaggccagga tcgtcggcgc
    19561 cgacaaacgc accgacgtgg cggtggtgaa gattgacgcc accggcctgc cggccgtgcg
    19621 cgtgggtgac gtgagtcgcc tgaaggccgg cgagtgggtc atggccattg gttcgccctt
    19681 tggcctggag aacacggtca cggccggcat cgtcagcgcc aagcagcgtg acacgggcga
    19741 ctacctgccc ttcatccaga ccgatgtggc catcaacccc ggcaactccg gtggcccgtt
    19801 gatcaacatg cgcggcgagg tcgtcggcat caacagccag atctattcgc gctccggcgg
    19861 cttcatgggc atctcgttcg cgataccgat ggacgaggcc atgcgcgtga gcgaacagct
    19921 gcgcaccagc ggccgcgtga gccgtgggcg catcggggtg cagatcgaat ccgtgtccaa
    19981 ggacgtggcc gagtcgatag gcctgggcaa gccacaaggg gcgttggtgc gtggcgtgga
    20041 ggtgggttcg cccgcagaga aggccggcat cgaggcgggc gacatcatta cccgctatga
    20101 gggcaaggcc gtggacaagg tggcggatct gccgcgcctg gtgggcaaca ccaagccggg
    20161 caccaaggcc agcatcaccg tgttccgccg tggcgccatg cgggatctgt ccatcaccat
    20221 tgccgagatc gagcccgatg agaaggtagc cgccaaatcg gccgatgccg agggcaaggc
    20281 caagccgtcc acggcggcac agcagctggg cctggtggtg gcggagctga cgccggcgca
    20341 ggccaaggag ctgaagatca agggcggcgt gcgcgtggtc tctgccgatg atgccgccgc
    20401 acgtgccggc ctgcgcgccg acgatgtcat cgtggccctg gccaataccg aggtgcgcag
    20461 cctgaaggat ttcgaagccg cgctggccaa ggtggacaag agcaagccca tcaacgtgct
    20521 gctgcgtcgt ggcgaatggg cgcagtacag cctgatccgc cccgcgcgtt gagccattga
    20581 gcgcttcccg gccgctatcc cgaagcctgc agtagggctt ttgccgccgg cagtgccctg
    20641 gggctgaagg tgctattcat gaatttcgat agaatggcgg ccctgaatcg gaggtgttcg
    20701 atatatattc gcggtctccg cgtcaccatg tgaaaacaca tgatgtcaat cacaggtgct
    20761 ccacagtttg tgcacatgtt gtccaaaact ggcgctctca atctgtggat taactgttga
    20821 taagtttccg ttgcagcatg gcaacgaccc tgtccgctag ctggccggcc tttccccaag
    20881 ggcctttttg cctacaatga ggctccaact atcgcagttg ccaaagattg ttggttcagg
    20941 gcgcgtcgct tatctgacgc gccctttttt cttgcccacg gtcttgccat tccatcattt
    21001 gtcgtatccc gttgatgaac cacatcagaa atttttcgat cattgcgcac attgatcacg
    21061 gcaagtccac gcttgctgat cgcatcatcc agcgctgcgg cgggcttgcc gagcgcgaga
    21121 tggaggccca ggtgctcgac tcgatggaca tcgagaagga gcgcggcatc accatcaagg
    21181 cgcagaccgc tgccctctca tacaaggcgc gcgacggaca ggtctacaac ctgaacctga
    21241 tcgacacgcc cggccatgtg gacttttcct acgaagtgag ccgatcgctg tcggcctgcg
    21301 agggggcgct gctggtggtc gatgcctccc agggcgtgga ggcgcagacc gtggccaact
    21361 gctacacggc gctggatctg ggcgtggaag tcctgcccgt gctcaacaag atggatctgc
    21421 cgcaggccga cccggacaac gccaaggccg agatcgagga cgtgatcggt atcgatgcgg
    21481 gtgaggcaat agccatttcg gccaagaccg gcatgggcat agacgatgtg ctcgagcaga
    21541 tcgtcgccaa ggtgccggcg ccgcgcggca agcccgaagc gccactgcgc gccatgatca
    21601 tcgacagctg gttcgacagc tacgtgggcg tggtgatgct ggtgcgtatc gtcgatggcg
    21661 aactgaagaa gggcgagcgc ttcaagatga tggccacggg cgccgcctat gaggccaacc
    21721 agcttggcgt cttcaccccc gctaacgtgc agcgcgatgc gctcaaggcg ggcgaggtcg
    21781 ggttcatcat cgccggcatc aaggagctga aggctgccaa ggtgggtgac accatcacgc
    21841 tggagaaaaa gctgcccaac aacctcggac ccgccaccga ggcacttccc ggcttcaagg
    21901 agatccagcc ccaggtgttc gccgggctct accccaccga ggccaaccag tacgaccagt
    21961 tgcgcgatgc gctggaaaag ctgcagctca acgatgcctc gctgcgtttc gagcctgagg
    22021 tgtcgcaggc actgggcttt ggttttcgct gcggctttct gggcctgttg cacatggaga
    22081 tcgtgcagga gcgcctggag cgcgagttcg accaggacct gatcaccacc gcacccagcg
    22141 tggtgtatga agtggtcaag ggcgacggcg agctgatcca ggtggaaaac ccctccaaga
    22201 tgcccgagca gggccgtatc caggaggtgc gcgagcccat cgtcacggtg cacctgtaca
    22261 tgccgcagga ctatgtgggg ccggtgatga cgctggccaa ccagaagcgc ggcgtgcaga
    22321 tgaacatggc ctaccacggc cgccaggtca tgctga
//
LOCUS       DAOMTQ010000032        21931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_272_length_21931_cov_26.243143, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000032 DAOMTQ010000000
VERSION     DAOMTQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 21931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21931
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_272_length_21931_cov_26.243143"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PK518_05035"
     CDS             complement(<1..1147)
                     /locus_tag="PK518_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRM48310.1"
                     /translation="MSYAQETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVK
                     GRKASRHATSDWMEIEKQRGISVASSVMQMSYRDHVINLLDTPGHKDFSEDTYRVLTA
                     VDSALMVIDAANGVEAQTRRLIEVCRQRDTPIITFVNKMDREVRDPLDILDEVERELG
                     MPCCPMTWPVGQGKSFGGIIDLRTQSMTVFQAGSEKRPEDFEVIPLSDVATLRARFGK
                     AYDDAIESMELAVGASIAWDHQEFLAARLTPVFFGSGVNNFGVMEVLNAVVDMSPPPG
                     ARIAYTEVNRQRERLTIHPEDKGFSGVVFKVQANMDANHRDRIAFVRVASGRYQPGMK
                     MKVQRTAKELRPTSVVTFMSQRREAVDEAYAGDIIGFTIHGGVQLGDS"
     gene            complement(1163..1471)
                     /locus_tag="PK518_05040"
     CDS             complement(1163..1471)
                     /locus_tag="PK518_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM48311.1"
                     /translation="MKVLRRHIRASRDIDQTIAYYLEHAPKGVADRFLDELELALQHI
                     AQHPGTGSRRYANASSIEGLRFWTLRHFPFAVFYAEHPHHIAVLRVLHQSSDLPQHLN
                     "
     gene            complement(1468..1737)
                     /locus_tag="PK518_05045"
     CDS             complement(1468..1737)
                     /locus_tag="PK518_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRM48312.1"
                     /translation="MSTVTMNISLSDELKAFVDARVQARGYSSTSEYMRDLVRRDEER
                     AAEERFAALIQEGVDSGIDPRPWEEHRAELRARIAAARNAESKSV"
     regulatory      1815..1907
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1983..3413
                     /gene="ahcY"
                     /locus_tag="PK518_05050"
     CDS             1983..3413
                     /gene="ahcY"
                     /locus_tag="PK518_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRM48313.1"
                     /translation="MSAVLKSPADSAIADITLAAWGRKEIKIAETEMPGLMAVREEFA
                     ASQPLKGARITGSLHMTIQTAVLIETLTALGADVRWASCNIFSTQDHAAAAIAATGVP
                     VFAIKGESLKDYWDYTHSIFEFGPAGAKDEGPNMILDDGGDATMLMHLGQRAEKDLSV
                     LANPGSEEEKIVFAAIRAKLAVDPTWYSRKSAQIMGVTEETTTGVHRLNEMSAKGLLK
                     FRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVACVAGYGDVGKGSAQAL
                     RALSAQVWVTEIDPINALQAAMEGYRVVTMEYAADKCDIFVTTTGNRDVITFEHMQAM
                     KNEAIVCNIGHFDNEIEVAKLEEHCQWDEIKPQVDHVIFKDGKKIILLAKGRLVNLGC
                     ATGHPSFVMSNSFANQTLAQIELFTRPDAYEVGKVYVLPKILDEKVARLHLKKVGAQL
                     TELSDAQAAYIGVAKTGPYKPDTYRY"
     gene            3601..4602
                     /locus_tag="PK518_05055"
     CDS             3601..4602
                     /locus_tag="PK518_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRM48314.1"
                     /translation="MRADVFLVEGGHASTRSQAQRLIAAGVQWRLAATLPWHKVAKNG
                     DDIPVGAELQLLDAAEAKYLSRGGLKLEGALAATGIQVAGLRCLDVGQSTGGFTDCLL
                     QAGAAQVIGVDVGQGQLHERLRADPRVIAVEGLNARHLTPQALQEGCEEALSVREELE
                     PDDNDTQPEAPYAWMRNGGMIDDEYDDSADAKEHEIEAFKAERATKAKDRAEGRLATV
                     RKRLPGREKDDITPAFDLVVGDLSFISLTLVLAALVPLLKPEGRLLMLVKPQFELQPG
                     QVGKGGIVRDASLYAEVEQRIRACCADIGLQVIGWHDSPIAGGDGNREFFVDARRAV"
     gene            4599..5426
                     /gene="metF"
                     /locus_tag="PK518_05060"
     CDS             4599..5426
                     /gene="metF"
                     /locus_tag="PK518_05060"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRM48315.1"
                     /translation="MSLPLSFEFFPAKTPEGADKLRTVRQQLYARAPQFCSVTYGAGG
                     STQAGTFAAVREILAEGVQAASHFSCIGATKDSVRAQLAELKAMGVKRLVALRGDLPS
                     GYGLGGEFHYASDLVAFIRAETGDDFHIEVAAYPEMHPQARSPEADLQAFAAKVRAGA
                     DSAITQYFFNADAYSRFVDESRRLGLDLPIVPGIMPITGSTQLMRFSDACGAEIPRWI
                     RLRLSGFGDDSASIRAFGLDVVSRLCEQLVAAGAPGLHFYTMNQSTATLAICQRLGR"
     gene            5669..>6078
                     /locus_tag="PK518_05065"
     CDS             5669..>6078
                     /locus_tag="PK518_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRM48316.1"
                     /translation="MRAPEVEPPLLLASGQASFYADSLHGRRTANGERYDREELTAAH
                     RSLPFGARLCVRSLVNGRTVIVRVNDRGPFTPGRIIDLSRAAAEELGMMGLGIKPVEL
                     WQLAAEDEACPESLEQAAQGSGLGLDEGVAARAL"
     assembly_gap    6079..6178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6211..7101)
                     /gene="rlmJ"
                     /locus_tag="PK518_05070"
     CDS             complement(6211..7101)
                     /gene="rlmJ"
                     /locus_tag="PK518_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRM48317.1"
                     /translation="MFSYRHAFHAGNHADVLKHTVLIATLQHLLQKDAALTVLDTHAG
                     AGLYRLDGDYATTSAESADGILRLVAPATPPALTPALQDYVDMVRAFNQGAVTRVYPG
                     SPFIAQRLLRDQDKLKLFELHPTDARALAGNVAQLEAGRQVAVLPEDGFEGVKKFLPP
                     PARRALVLCDPSYEIKSDYGRVLDLVQDSLKRFATGVYAVWYPIIPRPEAHDLPRRLK
                     TMANKAGKPWLHATLTVKSSKLAAPASENQRRPGLPASGMFLINPPFTLQAALREALP
                     QMAELLAQDRHATHGLEVGG"
     gene            7267..8889
                     /locus_tag="PK518_05075"
     CDS             7267..8889
                     /locus_tag="PK518_05075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRM48318.1"
                     /translation="MSVANHRATPAGVAQQSQALPPGTRLGEFEVVSLLGVGGFGMVY
                     QAFDHQLQRFVAIKEYMPAALAGRADGHSLWVRSSSDEQSFQAGLASFVDEARLLARF
                     EHPSLVKVLRFWEANHTAYMVMPLYCGMTLKQARAHMRTPPPEAWLRTLLWSVTSALR
                     VLHDRQTLHRDISPDNIFLQNHGPPVLLDLGAARHAISNRRQPAVLKVNYAPLEQYTD
                     APADMQQGPWSDLYALGATVHGCLRHGAPQPATQRAQRDRMLPMARVARQVRKQFGIE
                     YSSALVDAMAQCLALQPQDRPQSVAELLLAMEMDSAPPGLESFDFRAQLGASWVEPQD
                     RHAEGVAVPVVNTQIQTQASAPQQPAAPLPTHSQRRRRLRPHRSPQVRRTLWAMAGSA
                     AVLVMAAGLWWEQKPRAPRQTPESEIITELAETEEPRPLAPMVQPARQAMDGGMRQAH
                     PAPAPELPASTLEPMPQPGLVVLEAAMPAAPQAHPRAGNPLDSCASAGFWARSMCIHQ
                     ACQEDGIANHPVCVDERVRREAQERQRQLYGQ"
     gene            9044..9472
                     /gene="rplM"
                     /locus_tag="PK518_05080"
     CDS             9044..9472
                     /gene="rplM"
                     /locus_tag="PK518_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRM48319.1"
                     /translation="MSTFSAKPAEVVHEWFVIDATDKVLGRVASEVAHRLRGKHKAIY
                     TPHVDTGDFIVIVNASKLKVTGTKSTDKVYYRHSGYPGGITATNFRDLQAKHPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYGGAEHPHSAQQPKPLEI"
     gene            9482..9874
                     /gene="rpsI"
                     /locus_tag="PK518_05085"
     CDS             9482..9874
                     /gene="rpsI"
                     /locus_tag="PK518_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRM48320.1"
                     /translation="MIGEWNNGTGRRKSSVARVFLKKGSGKITVNGKDIAQYFGRETS
                     IMITKQPLVLTGNVEAFDIQINVHGGGESGQAGAARHGITRALIDYDAALKPQLSAAG
                     FVTRDAREVERKKVGLHSARRAKQFSKR"
     gene            10038..10412
                     /gene="erpA"
                     /locus_tag="PK518_05090"
     CDS             10038..10412
                     /gene="erpA"
                     /locus_tag="PK518_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRM48321.1"
                     /translation="MSAVAQTSDTEMPTPILFTDSAAAKVAELIAEEGNPELKLRVFV
                     QGGGCSGFQYGFTFDEITNEDDTTMTKNGVSLLIDAMSFQYLVGAEIDYKEDLQGAQF
                     VIKNPNADTTCGCGSSFSTPEH"
     gene            complement(10468..11577)
                     /locus_tag="PK518_05095"
     CDS             complement(10468..11577)
                     /locus_tag="PK518_05095"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRM48322.1"
                     /translation="MTQWYIGLMSGTSLDGVDGVLAEFSGQKCRVAQYSSAQIAPDLR
                     AELLALNTPGNDELHRAALAANALVRGYADVVQRLLAQAGLRPDAIHAIGAHGQTVRH
                     RPLAFDGTGYTLQLNNPALLAEITGITVVADFRSRDVAAGGQGAPLVPAFHQGMFGQA
                     GRSVGVLNLGGISNLSLLRADGGVLGFDCGPANALMDYWCERHTGRTYDDQGAWARTG
                     RPHGALLDALLAEPFLALPPPKSTGRDLFNPHWLEPLLAAASNALPQDVQATLAEFTA
                     RSCAQDLLRHASDCDLLIVCGGGALNQYLMERLQALLPGVTVQDSALHGLPPLQVEAA
                     AFAWLARQCLAGQTASLPEVTGARGARILGAIYPA"
     gene            complement(11592..12956)
                     /locus_tag="PK518_05100"
     CDS             complement(11592..12956)
                     /locus_tag="PK518_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRM48323.1"
                     /translation="MNNGLTTAGLLLLSQLKQTLQTHPKRITAALTTLLLTTGGGAFA
                     VASLAPDPAELPVSTVTQAVQSLAEGEPLSALVDGPGFSLYRSDVTRGADTAESILQR
                     LGVADPAAAAFLRADNAVRQNLLGRVGRSMSAEATDDHRLVRLTARWAQDDSDRFQRL
                     VVERKPDGSFASRIESAPLTVGSRLAGGVIRSSLFAATDAASIPDAVAVQLAEVFSGD
                     IDFRRALRKEDRFSVVYETLEADGEPLRSGRVLSAEFQNGEKTYNALWFQPPGAAKGA
                     YYTLDGQSMRRAYLTSPVEFSRVSSGFAMRLHPIHKTWRAHLGTDFAAPTGTAVRTVG
                     DGVVDFAGVQNGYGNVVYVKHRNQHVTIYAHLSRIDVRKGDSVEQGQKLGAVGATGWA
                     TGPHLHFEFRVNGEARDPLSIARESEAAQPVSNAARPAFNRLASNMRVQLSSAVQILQ
                     ASAE"
     gene            13148..14380
                     /gene="tyrS"
                     /locus_tag="PK518_05105"
     CDS             13148..14380
                     /gene="tyrS"
                     /locus_tag="PK518_05105"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRM48324.1"
                     /translation="MNQTVVTNFPVTDDVRQALEVSLRGVDELLPREDWTRKLARSAA
                     TGQPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHQVIFLIGDFTTLIGDPSGRNS
                     TRPPLTAEQIKVNAETYYTQAAKVLDPARTEIRYNSEWNDALGTRGMIQLAAKYTVAR
                     MMERNDFHQRFTEGSSISLHEFLYPLLQGYDSVALKADLELGGTDQKFNLLMGRHLQQ
                     EWGQEPQCVLTMPLLVGLDGVEKMSKSKNNYIGITEDANTMFAKVLSISDELMWDWYT
                     LLSFRSLAEIASLRQEVAEGRNPKDAKVLLAKEITARFHSAAAADAAEEDFNNRAKGG
                     IPDDIPDLQLCGAPLGIAALLKQAGLAPSSSEANRLIDGGGVRVDGGVVSDKGLKLAT
                     GRYVVQVGKRKFARVTLA"
     gene            complement(14461..15453)
                     /locus_tag="PK518_05110"
     CDS             complement(14461..15453)
                     /locus_tag="PK518_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM48325.1"
                     /translation="MKTPLPPITHLRTRPVAVGHWRAFLAVARHLNFRAAAEELALTQ
                     SAVSRQIQALEDEVGVPLFLRHTRAVELTGAGGQLQRAVAPALERMDAAVRLVRQTAG
                     RKSVAITTWASFASMWLIPRMEEFQRDNPDIDIRMDASDAMVDLETADVDLALRSAVP
                     GSQPAGAERLFGEQLAVVASPWLLKSMPPIHEPADVARFTLIEAGDAHRLPYFELITW
                     RRWFQVHGLEKLQPRRWLYFNYAQQIAQAALAGQGLALARMPLVAQSLASGDLVEVLP
                     GHRLDSPLAYWLVVGPRSGQRPEIQAFCAWLLLQAETTRRAIGEAAPVTTPDTP"
     gene            15575..15880
                     /locus_tag="PK518_05115"
     CDS             15575..15880
                     /locus_tag="PK518_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HRM48326.1"
                     /translation="MTASHVLNSQQWGACADAAGADPYRLATGAARSLRPTTAMRLRV
                     VCGQAWVTVDYGPHGWREDSGDLLLQAGQSLRIAPGQHAVVEPLGQQALQYQWCSAG"
     gene            15942..17369
                     /gene="glmU"
                     /locus_tag="PK518_05120"
     CDS             15942..17369
                     /gene="glmU"
                     /locus_tag="PK518_05120"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRM48327.1"
                     /translation="MTALDIIIMAAGKGTRMKSRTPKVLQRLAGRPLLQHVLDQAASL
                     QARRVVVITGHGAAEVEASCAQRTGASGQFDLKCVRQEPQLGTGHAVQQAVPQLAGDG
                     MVVVLSGDVPLTQAATLAALVDAAGGERLALLTVQLSDPTGYGRIVRAANDQVQRIVE
                     HKDASAEQRAIGEIYSGIMAVPARLLAGWLARLTNDNAQGEYYLTDIVAMAVADGVPV
                     VAHRIQDALQVAGVNSPVQLAELERAHQMRQAAALMEQGVRLADPARFDLRDDAKTGA
                     RGELICAQDVDIDVGCIFTGRVELGEGVQIGAYCCIANASIAAGAVIHPFTHIDGEKA
                     GASVGEGALVGPFARLRPGAQLGREVHIGNFVEVKNSTLADGAKANHLAYLGDATVGE
                     RVNYGAGCITANYDGANKHRTVIEADVHVGSNCVLVAPVILGAGGTVGGGSTITKDTP
                     AGALSVARGKQVNIAGWKRPVKQAK"
     gene            17375..18178
                     /locus_tag="PK518_05125"
     CDS             17375..18178
                     /locus_tag="PK518_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM48328.1"
                     /translation="MQSDAAATETPEQLRVALDQAQRQIADMAAAHEAFLRAVSHDLR
                     APLRHVTSYGALARELLQELSQELPGQPPQLLEAMDFLATMEGSARRMALMIDGLQAI
                     ARAGRAPLRLQSLDLAGAVQQARAALGGAADGVQWQIAGAMPPVQADAELLTQLLTQL
                     LANALKFSRGVAQPRIALHAEVAPAGRVHVTLQDNGVGFDGTRAQQLFGVFQRLHREA
                     DFDGVGAGLALCQAIAQRHGASISATAAPGAGCCIRLDWPGAAAPGCSG"
     gene            complement(18170..18499)
                     /locus_tag="PK518_05130"
     CDS             complement(18170..18499)
                     /locus_tag="PK518_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRM48329.1"
                     /translation="MVTHHFLDRALAELLDEPNRLTLEELARSCCMAPGWVVERLEAG
                     LLQGEQSGGRWLFTSSTVLRARRLARLESSFDADPELAALTADLIEEVAVLRQRLRQL
                     EQGVDLP"
     gene            complement(18502..19485)
                     /locus_tag="PK518_05135"
     CDS             complement(18502..19485)
                     /locus_tag="PK518_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRM48330.1"
                     /translation="MEFKDYYQILGVPRDAGADDIKKAYRKLARRYHPDVSKEADASA
                     RMAEVNEANTVLSDPEKRAAYDRLGQEAAHHPGQDFRPPPNWDAGFEFTGAPGAEGLD
                     GAEFSDFFEQLFGRAARAQHAQGRGGAQARGAPPPQRGRDHHASIELDLRDAYHGGEQ
                     LLALHGARLDAAGHLVNEQRQLQVTIPKGVRAGQLIRLSGQGGPGTPAGDLFLEVRFK
                     PDTRWRAEDRDVYQAVTLAPWEAELGGAIEVQTPGGATVEVTVPPRWKPGRKLRLRER
                     GIPAATPGDLYLELHVALPAAATPAQQQAYRALAQAFPDFKPRATQGAQGG"
     gene            complement(19584..20078)
                     /locus_tag="PK518_05140"
     CDS             complement(19584..20078)
                     /locus_tag="PK518_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM48331.1"
                     /translation="MESDFIPDATDWQLLDWLQRDASLSNQALAERVHVSPPTCLRRV
                     RRLQESGLIERQVALLQPDRLAALQGHGLAAIVEVSLDRQGAEHLDAFQARASADDAV
                     QQCWRVSPGPDFVLVVHTRDMPAYLALSQRLFTQDANVRNVKVFFATQRAKFETKLPI
                     AHTQ"
     gene            20217..>21931
                     /gene="glmS"
                     /locus_tag="PK518_05145"
     CDS             20217..>21931
                     /gene="glmS"
                     /locus_tag="PK518_05145"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRM48332.1"
                     /translation="MCGIVGAVSTRNIVPILVQGLQRLEYRGYDSCGVAVHEASMGQH
                     PAGGLRRARSTARVAELLEQVAQDDLQGATGIAHTRWATHGAPAVHNAHPHFSHGCGE
                     APAETARAGRVALVHNGIIENHEQLRARLQARGYVFSSQTDTEVIAHLVDSLYDGDLF
                     QAVRAATAELHGAFAIAVIHKDEPQRVVGARAGSPLVLGVGQGENFLASDAMALAGVT
                     DQIVYLEEGDLVDLQLGRYWITGPDGQPLAEGSRPVRTVHAHSGAAELGPYRHYMQKE
                     IFEQPRAIADTLDGVQAIVPELFDGAGQHGQPGQAAWRVFKEIDSVLILACGTSYYSG
                     CTAKYWLESIAGIPTQVEVASEYRYRTSVPNPRTLVVTISQSGETADTLAALRHAQGL
                     GMKHTLTICNVATSAMVRECALTYVTRAGVEIGVASTKAFTTQLAGLFLLTLALAQTK
                     GRLTEEKEAQYLTAMRHLPAALQAVLALEPQIISWAEDFAKMENALFLGRGIHYPVAL
                     EGALKLKEISYIHAEAYPAGELKHGPLALVTSSMPVVTVAPNDELLEKLKSNMQEVRA
                     RGGVL"
BASE COUNT         3641 a         7259 c         7466 g         3465 t
ORIGIN      
        1 tgctgtcgcc cagctgcacg ccgccgtgga tggtgaagcc gatgatgtcg ccggcgtagg
       61 cttcatctac ggcctcgcgg cgctggctca tgaacgtgac cacgctggtc gggcgcagct
      121 ccttggccgt gcgctgcacc ttcatcttca tgccaggctg gtagcggccc gacgccacgc
      181 gcacgaaggc gatgcggtcg cggtggttgg cgtccatgtt cgcctgcacc ttgaagacca
      241 cgccggagaa ccccttgtcc tcggggtgaa tcgtgagccg ttcgcgctgg cggttcacct
      301 cggtgtaggc gatgcgcgct cccggcggtg gcgacatgtc caccaccgca ttgagcacct
      361 ccatgacacc gaagttgttc acgcccgaac cgaagaacac gggcgtcagg cgcgcggcca
      421 gaaactcctg gtggtcccag gcgatggacg cgccgacggc cagctccatg ctttcgatgg
      481 catcgtcgta ggccttgcca aagcgcgcgc gcagcgtggc gacgtcgctc agggggatga
      541 cctcgaagtc ctccgggcgc ttctcgctgc cggcctggaa caccgtcatg ctctgcgtgc
      601 gcaggtcgat gatgccgccg aagctcttgc cctggcccac cggccaggtc atggggcagc
      661 agggcatgcc cagctcgcgc tcgacctcgt cgaggatgtc gagcgggtcg cgcacctcgc
      721 ggtccatctt gttgacgaag gtgatgatgg gcgtgtccct ctggcggcag acctcgatca
      781 ggcggcgcgt ctgcgcctcc acgccgttgg cggcgtcgat caccatcagc gccgagtcaa
      841 ccgcggtgag cacgcggtag gtgtcctcac tgaagtcctt gtggccgggt gtgtccagca
      901 ggttgatgac gtgatcgcgg tagctcatct gcatcaccga agacgccacc gagatgccgc
      961 gctgcttttc gatctccatc cagtcggacg tggcgtggcg gctggccttg cggcccttga
     1021 cggcgccggc gatctggatc gcgcccgaga acagcagcag cttttcggtc agcgtggtct
     1081 tgccggcgtc ggggtgggaa atgatggcga aggtgcggcg gcgccgggtt tcttgggcgt
     1141 aggacacgaa gttctcgatg gttcaattca agtgctgggg caggtctgac gactggtgca
     1201 agacgcgcag cacggcgata tggtgggggt gctcggcgta gaacacggca aaaggaaagt
     1261 ggcgcaacgt ccaaaaacgc agcccctcga tcgaggaggc gttggcgtag cgcctggagc
     1321 ctgtgcctgg gtgctgcgca atgtgctgca aagccagttc gagctcgtcc agaaatcgat
     1381 cggccacgcc cttgggagcg tgttccagat agtaggcgat ggtctgatcg atgtcgcggc
     1441 tggcccggat gtggcggcgc agcactttca cacggatttg ctttcagcgt tgcgtgctgc
     1501 ggcaatgcgt gcgcgcagtt cggcacggtg ctcctcccaa ggccgtggat cgatgcccga
     1561 atcaacgccc tcctggatca gcgccgcgaa gcgctcctct gcggcgcgct cctcgtcgcg
     1621 gcgcaccagg tcgcgcatgt actcgctggt gctgctgtag ccgcgcgcct gtacgcgggc
     1681 atcgacaaag gccttcagct cgtcgctcag agagatgttc atggtgaccg tgctcatggg
     1741 gcgtgatttt acgagtcttg gcaaggattg ccaaaaaccg taggtagaaa ccagcacccc
     1801 ggtataatcg cgcgctttcc gaggagcgtt gcagcgtccc ccacctggca gcagggcaag
     1861 cggggcgtga ggctcggaac ccactcttgc aacgacgctc acccacgcca ggcctgtgcg
     1921 gtgaacggtt ttcatgcttt ccccaggcgc gtggtttttt cccattcgtg aaggaacaca
     1981 ccatgagcgc agttctcaaa tctcccgctg actcggccat tgccgacatc accctggccg
     2041 cctggggccg caaggaaatc aagattgccg agaccgagat gcccggcctg atggcggtgc
     2101 gcgaggagtt cgccgccagc cagccgctca agggcgcgcg catcaccggc agcctgcaca
     2161 tgaccatcca gaccgccgtg ctgatcgaga cgctcacggc cctgggcgcc gacgtgcgct
     2221 gggcctcgtg caacatcttc tcgacgcaag accacgccgc cgccgcgatt gccgccaccg
     2281 gcgtgccggt gtttgccatc aagggcgagt cgctgaaaga ctattgggac tacacgcaca
     2341 gcatctttga atttggcccc gccggtgcca aggacgaagg cccgaacatg atcctggacg
     2401 acggcggcga cgccaccatg ctgatgcacc tgggccagcg cgctgagaag gatttgtcgg
     2461 tgctggccaa tcctggctcc gaagaagaga agatcgtttt cgccgccatc cgcgccaagc
     2521 tggccgtgga ccctacctgg tacagccgca agagcgcgca gatcatgggc gtgaccgagg
     2581 agacgactac cggcgtgcac cgcctgaacg agatgtcggc caagggcctg ctcaagttcc
     2641 gcgccatcaa cgtcaacgac tcggtgacga aatccaagtt cgacaacctc tacggctgcc
     2701 gcgaatcgct ggtggacggc atcaagcgcg ccaccgacgt gatgatcgcc ggcaaggtgg
     2761 cctgtgtggc cggctatggc gacgtgggca agggcagcgc ccaggcgctg cgcgctttga
     2821 gcgcccaggt ctgggtgacc gagatcgacc ccatcaacgc cttgcaggca gcgatggaag
     2881 gctaccgcgt ggtgaccatg gaatacgccg ccgacaagtg cgacatcttt gtgacgacca
     2941 ccggcaaccg cgacgtgatc acgtttgagc acatgcaggc catgaagaac gaggccatcg
     3001 tgtgcaacat cggccacttc gacaacgaga tcgaggtcgc caagctggaa gagcactgcc
     3061 agtgggacga gatcaagccc caggtcgatc acgtcatctt caaggacggc aagaagatca
     3121 tcctgctggc caagggccgc ctggtgaacc tgggctgcgc caccggccac cccagcttcg
     3181 tcatgagcaa ctcgttcgcc aaccagacgc tggcccagat cgagctgttc acgcgcccgg
     3241 atgcgtacga agtgggcaag gtctatgtgc tgcccaagat tctggatgaa aaggtggcac
     3301 gcctgcacct gaagaaggtg ggcgcgcaac tgaccgagct ctccgacgcg caggcggcct
     3361 atatcggcgt cgccaagacc ggcccgtaca agcccgatac ctaccgctat tgatggcgtc
     3421 caactccctc tcccccgtgg gggagagggc aggggtgagg gggagtacag gcgcagtgct
     3481 ggctaccagc acaccctcgc cccaccctct cccgccagcg ggagagggag taaaaccata
     3541 gctgtccacg ctttccagta aagcgttaca gccctatttg attcataaag gacccgcgcc
     3601 atgcgcgcag acgtatttct ggtggagggc ggccatgcca gcacccgctc gcaggcccag
     3661 cgcctgatcg ccgccggcgt gcagtggcgc ctggcggcta cgctgccgtg gcacaaggtg
     3721 gccaagaatg gcgacgacat tccggtcggg gccgagctgc agttgctcga cgcggccgag
     3781 gccaaatatc tctcgcgcgg cgggctcaag ctggaagggg cgttggccgc caccggcatc
     3841 caggttgccg gcctgcgctg cctggacgtg ggccaaagca ccggcggctt caccgactgc
     3901 ctgctgcagg ccggcgccgc gcaggtgatt ggcgtggacg tgggccaggg ccagctgcac
     3961 gagcgcctgc gtgccgatcc gcgcgtcatc gccgtcgaag gcctgaacgc acgccacctc
     4021 acgccccagg cgctgcagga gggttgcgaa gaagcgctca gcgtgcgcga ggagcttgag
     4081 cccgacgaca acgacaccca gcccgaagct ccctacgcct ggatgcgcaa cggcggcatg
     4141 atcgacgacg agtacgacga cagcgcagac gccaaggagc acgagatcga agccttcaag
     4201 gccgagcgcg ccaccaaggc caaggaccgc gccgaaggcc ggctggcaac ggtgcgcaag
     4261 cgcctgcccg ggcgcgagaa ggacgacatc acgcccgcct tcgacctcgt cgtgggcgac
     4321 ctgtccttca tctcgctgac gctggtgctg gcggccctgg tgccactgct caagcctgaa
     4381 ggccgtctgc tgatgctggt caagccgcag ttcgagctgc agcccggcca ggtgggcaag
     4441 ggcggcatcg tgcgcgacgc cagcctgtac gccgaggtgg agcagcgcat ccgcgcctgc
     4501 tgcgcagaca ttggcctgca ggtgatcggc tggcatgaca gccccatcgc tggcggcgat
     4561 ggcaatcgcg aattttttgt tgatgcaagg agggccgtat gagcctgccg ctgagtttcg
     4621 agttctttcc cgccaagacg cccgaagggg cagacaagtt gcgcaccgtg cgtcagcagc
     4681 tgtatgcgcg cgcgccgcag ttctgctcgg tcacctatgg cgctggcggc tccacccagg
     4741 ccggcacctt cgccgcggtg cgcgagattc tggccgaggg ggtgcaggct gcctcgcatt
     4801 tctcctgcat aggcgccacc aaagacagcg tgcgcgcgca gctcgccgaa ctgaaggcca
     4861 tgggcgtgaa gcgcctggtg gcgctgcgcg gcgacctgcc cagcggctac ggcctgggcg
     4921 gcgaattcca ctacgccagc gacctggtgg ccttcatccg cgccgagacg ggcgatgact
     4981 ttcatatcga ggtggccgcc taccccgaga tgcacccgca ggcgcgctcg cccgaggccg
     5041 atctgcaggc cttcgccgcc aaggtgcgcg ccggggccga ctcggccatc acgcagtact
     5101 tcttcaacgc cgacgcctac agccgcttcg tcgatgagag tcgccgcctg ggcctggacc
     5161 tgcccatcgt ccccggcatc atgccgatca ccggctcgac ccagctgatg cgcttctccg
     5221 atgcctgcgg cgccgagatt ccgcgctgga tacgcctgcg cctctcgggc tttggcgatg
     5281 acagcgccag catccgcgcc ttcgggctgg acgtggtctc gcgcctgtgc gagcagctcg
     5341 tcgcagccgg tgcgccgggc ctgcacttct acaccatgaa ccagagcacg gccacgctgg
     5401 ccatctgcca gcggctgggg cgttgatgcg aagcggcggc agccccaggt tgcgccgcca
     5461 gcctgggctg ccagcggttt tggtggcggc ctggctggcg ggctgtgccg cgccacagcc
     5521 acccgcacct gtgaccacca ccgcagcgcc cgttgttgcg gcgccgcagt ccagcgccgc
     5581 ggtgccggcg ccgccgcagg agcgacccgc caccgcgctg cgcatcaagc cccagcgacc
     5641 ccccggtgaa ggcgcgggcg caccggccct gcgtgcgccg gaggtcgagc cgccgctgtt
     5701 gctggccagc ggccaggcct cgttctatgc cgacagcctg cacgggcggc gcaccgccaa
     5761 cggtgagcgc tacgaccgcg aggagttgac ggccgcgcac cgcagcctgc cctttggcgc
     5821 gcgcctgtgc gtgcgcagcc tggtcaacgg ccgcacggtc atcgtgcgcg tgaacgaccg
     5881 cggaccgttc acgcccgggc gcatcatcga cctgagccgc gccgcggccg aggagctcgg
     5941 catgatgggc ctgggcatca agccggtcga gctgtggcag ctggccgccg aggacgaggc
     6001 ctgccccgaa agcctggagc aggcagccca gggctcgggc ctggggctgg acgaaggggt
     6061 ggccgcgcgc gccttgcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     6181 cgcaagcgcc ggtgaccctg atgtccagga tcagccgccg acctccagcc catgggtcgc
     6241 atgccggtcc tgggccagca gctcggccat ctgcggcagg gcctcgcgca gcgcggcctg
     6301 cagcgtgaac ggcgggttga tcaggaacat gccgctggcc ggcaggccgg ggcggcgctg
     6361 gttttcgctg gcaggggcgg ccagcttgct ggatttcacc gtcagcgtgg cgtgcagcca
     6421 gggcttgccg gccttgttgg ccatggtctt caggcggcgc ggcaggtcat gtgcctcggg
     6481 cctggggatg atggggtacc agacggcgta cacgccggtg gcaaagcgct tcaggctgtc
     6541 ctgcaccagg tccagcacac gcccgtagtc gctctttatt tcatagctag ggtcgcacaa
     6601 tacaagcgcg cggcgggccg gtggaggcaa aaatttcttg acaccctcaa aaccgtcctc
     6661 gggcagcacg gccacctggc ggccggcctc cagctgggcc acgttgccgg ccagggcgcg
     6721 tgcgtcggtg gggtgcagct cgaacagttt cagcttgtcc tgatcgcgca gcaggcgctg
     6781 ggcaatgaag ggcgagccgg gatagacgcg cgtgaccgcg ccctggttga aggcgcgcac
     6841 catgtccaca tagtcctgca gcgcgggcgt cagcgccggc ggcgtggcgg gcgccaccag
     6901 gcgcagaatg ccgtcggcgg attcggcgct ggtggtcgca tagtcgccgt ccagccggta
     6961 cagacccgcc cctgcatgtg tatccagtac ggtcaaagcc gcgtcttttt gtagaaggtg
     7021 ttgcagggtg gcaatcagca ccgtgtgttt gagcacgtcg gcgtggttgc ctgcatggaa
     7081 ggcgtggcga taactgaaca tgcgcgcaat ggtagcggcc cgcaagcgcg ccgcaagcac
     7141 gcgacccgtt tggtggggtg ttgccatggg ccgacatgga tgcgctttgt tgcttgtcgg
     7201 ggcgggccct gggccatgat cacccttctc ttgcgccagc aacccgcacc cgttctgccc
     7261 cttcccatgt ccgtagctaa ccatcgcgcc acgccggccg gtgtggcgca gcagtctcaa
     7321 gccctgccgc cgggaacgcg gctgggcgag ttcgaggtgg tgtctctgct gggggtgggc
     7381 ggctttggca tggtctatca ggcgttcgat caccagttgc agcgtttcgt ggccatcaag
     7441 gagtacatgc cggcagcgct cgccgggcgc gccgacgggc attcgctgtg ggtgcgctcg
     7501 tcgtcggacg agcagtcgtt ccaggccggc ctggcctcgt tcgtggacga ggcgcgcctg
     7561 ctggcgcgct ttgagcaccc cagcctggtg aaggtgctgc gcttctggga ggccaatcac
     7621 accgcctaca tggtcatgcc gttgtactgc ggcatgacgc tcaagcaggc gcgcgcgcac
     7681 atgcgcacgc cgccgcccga ggcctggctg cgcacgctgc tgtggtcggt caccagcgcc
     7741 ctgcgcgtgc tgcacgatcg gcagaccctg caccgcgaca tctccccgga caacatcttt
     7801 ttgcagaacc acggcccgcc ggtgctgctg gatctggggg cggcgcgcca cgccatcagc
     7861 aaccgcaggc agccggcggt gctgaaggtg aactacgcgc ccctggagca gtacaccgat
     7921 gccccggccg atatgcagca agggccctgg agcgatctgt atgccctggg cgcgacggtg
     7981 catggctgcc tgcgccacgg cgccccccag ccggccacgc agcgcgcgca gcgtgaccgc
     8041 atgctgccca tggcacgcgt ggcacgccag gtgcgcaagc agttcggcat cgagtattca
     8101 tcggcgctcg tggatgccat ggcgcaatgt ctggcgctgc agccgcagga caggccgcag
     8161 tccgtcgctg agctcctgct ggccatggag atggacagcg ccccgccggg gctggagtcg
     8221 tttgattttc gggcgcagct tggcgccagc tgggtcgagc cgcaggacag gcatgccgag
     8281 ggcgtggctg tgccggtggt caacacccag atccagacgc aggccagcgc gccacaacag
     8341 cctgcagcac ccttgcccac acactcgcag cgtcggcgcc gcctgcgccc ccaccgtagc
     8401 ccgcaggtgc gccggacgct atgggccatg gccggctcgg ccgcggtgct tgtcatggca
     8461 gccggcctgt ggtgggagca gaaaccgcgc gcgccgcgcc agacgcccga gagtgaaatc
     8521 atcaccgagc tggccgagac cgaagagccc cgtccactgg cgcccatggt gcagccagcg
     8581 cgccaggcca tggatggcgg catgcgccag gcgcatcccg cgcctgcgcc agagctgcct
     8641 gcatcgaccc tggagccgat gccccagccc gggctggtgg tgctggaggc ggccatgccc
     8701 gccgcgccgc aggcgcaccc gcgggcgggc aatccgctgg acagctgtgc cagcgccggc
     8761 ttctgggcgc gcagcatgtg catccaccag gcctgccagg aagacggcat cgccaaccac
     8821 ccggtttgcg tggacgaacg cgtgcgtcgc gaggcacagg agcgccagcg ccagctgtat
     8881 gggcagtagg ccggcatttt tgtataatgg cgggcttcgc agcgaatttt ggtcccgcgg
     8941 cgaggcatga aattcttcat gcacccacct aagaggctcc cagtcacgag gagcaccgac
     9001 cgtggaaaag gaccgtcaaa ccccctcttg caaagagaaa ctcatgtcca ctttcagcgc
     9061 caagcccgct gaggtcgtgc acgagtggtt tgtgattgac gccaccgata aggtgctcgg
     9121 ccgggtcgcc agcgaagtgg cccaccgtct gcgcggcaag cacaaagcca tttacacgcc
     9181 tcacgtcgat accggtgact tcatcgtcat cgtcaacgcc tccaagctca aggtcaccgg
     9241 caccaagtcc accgacaagg tgtactaccg tcactcgggc tatcccggcg gcatcacggc
     9301 caccaacttc cgcgatctgc aggccaagca ccccggccgc gcgctggaga aggccgtcaa
     9361 gggcatgctg cccaagggcc ctctgggcta cgccatgatc aagaagctca aggtctacgg
     9421 tggcgcggag catccgcaca gcgcccagca gcccaagccc ctggaaatct aaggagccga
     9481 catgattggt gaatggaaca atggcaccgg ccgtcgcaag tccagcgtcg cccgcgtgtt
     9541 tctgaagaag ggctccggca agatcactgt gaatggcaag gacattgcac agtatttcgg
     9601 ccgcgaaacc tcgatcatga ttaccaagca gccgctggtg ctgactggca atgtcgaagc
     9661 cttcgacatc cagatcaacg tccacggtgg tggtgagtcc ggccaggccg gcgccgcccg
     9721 ccatggcatc acccgcgccc tgatcgacta cgacgcagcg ctcaagcccc agctgagcgc
     9781 cgccggcttc gtcacgcgcg acgcccgtga agtcgagcgc aagaaggtcg gcctgcactc
     9841 cgcacgccgc gccaagcagt tctccaagcg ttaatccgca cggttttctg cagccgcatc
     9901 aaggccacct tcgggtggct ttgtgcttta tacggtcagg caaaagcccc aggggttttg
     9961 gcagcaagtc gccatgaacc ggagttggcc gcatgtttgc agtagcatcc aacaagtttt
    10021 gaacattgga gcaatccatg agcgcagtag cacaaacctc cgacaccgag atgcccaccc
    10081 cgatcctttt caccgacagc gcggctgcca aggtggccga gctgatcgcc gaggagggca
    10141 accccgaact gaagctgcgc gtgttcgtgc agggcggcgg ctgctcgggc tttcagtacg
    10201 gttttacctt cgacgagatc accaacgagg acgacaccac catgaccaag aatggcgtgt
    10261 cgctgctgat cgacgccatg agcttccagt acctggtggg cgccgagatc gactacaagg
    10321 aagacctgca gggcgcgcag ttcgtcatca agaacccgaa tgccgacacc acctgcggct
    10381 gcggctccag cttctccacg cccgagcact gacaaacttc caaccgtaca ggggcaaaaa
    10441 aaccgctgca aggcggtttt tttccgttca tgcggggtag atggcgccca ggatgcgcgc
    10501 gccgcgtgcg ccggtcactt cgggcaggct ggccgtctgg ccggccaggc attgtcgtgc
    10561 cagccaggcg aaggcggcgg cctcgacctg cagtggcggc agaccatgga gggccgaatc
    10621 ctgcacggtg accccgggta gcagggcctg caggcgctcc atcagatact gattgagcgc
    10681 gccgccgcca cagacgatga gcagatcgca gtcgctcgcg tggcgcagca agtcttgtgc
    10741 gcagcttctg gcggtgaact cggccagcgt cgcctgcacg tcctgcggca gggcgttgct
    10801 ggctgctgcc agcagaggct ccagccagtg cgggttgaac agatcccgcc cggtgctttt
    10861 gggcggcggc agggccagaa agggctcggc cagcagggca tcgagcaggg cgccatgggg
    10921 ccggccggtg cgcgcccagg caccctggtc gtcgtaggtg cggccggtgt ggcgctcgca
    10981 ccaatagtcc atcagcgcgt tcgccgggcc gcagtcaaaa cccagcacgc cgccatcggc
    11041 gcgcagcaga ctcagattgg agatgccacc caggttgagc acgcccacgc tgcgccctgc
    11101 ctggccaaac atgccctggt ggaaggcggg caccagcggc gcgccctggc cgccggcggc
    11161 gacatcacgg ctgcgaaagt ccgccaccac ggtgatgccg gtgatttccg ccagcagggc
    11221 ggggttgttc agctgcaggg tgtagcccgt gccatcgaag gccaggggcc ggtgacgcac
    11281 ggtctggcca tgcgcgccta tggcgtggat ggcatctggc ctcagcccgg cttgcgccag
    11341 caggcgttgc accacatctg catagccgcg taccagggca ttcgcggcca gtgcggcgcg
    11401 gtgcagttca tcattgccgg gggtgttaag tgcgagcagt tctgctctta aatcaggagc
    11461 tatttgcgct gatgagtact gcgctacacg gcatttttgc cctgaaaact cggccagcac
    11521 gccatcgacc ccgtcgagcg aggtgcccga catcaggccg atgtaccact gcgtcaaagg
    11581 agggcgtggc cttattcggc gctggcctgc aggatttgca cggccgatga cagctgcacg
    11641 cgcatgttcg acgccaggcg gttgaaggcc gggcgtgcgg cgttcgacac gggctgggcg
    11701 gcctcgcttt cacgggcgat gctcagcggg tcgcgggcct cgccattgac gcggaactcg
    11761 aagtgcagat gcgggcccgt ggcccagccg gtggcgccca cggcgcccag cttttgcccc
    11821 tgctcgacgc tgtcgccctt gcgcacgtcg atacggctca ggtgagcgta gatggtgaca
    11881 tgctggttgc ggtgcttgac gtagaccacg ttgccgtagc cgttctgcac gccggcaaag
    11941 tccaccacgc cgtcgcccac ggtgcgcacc gccgtgcccg tgggcgcggc gaagtcggtg
    12001 cccagatggg cgcgccaggt cttgtggatg gggtgcaggc gcatggcaaa accactggag
    12061 acgcgcgaga actcgaccgg cgaggtcagg taggcgcggc gcatgctttg cccgtccagc
    12121 gtgtagtacg cacccttggc ggcgccaggc ggctggaacc agagggcgtt gtaggtcttc
    12181 tcgccgttct ggaactcggc gctgagcacg cggccgctgc gcagcggctc gccgtccgcc
    12241 tccagcgtct cgtagaccac ggagaagcga tcctccttgc gcagcgcgcg gcggaagtcg
    12301 atgtcccccg agaacacctc ggccagctgc acggccacgg catcggggat gctggccgcg
    12361 tcggtggcgg caaacagcga gctgcgaatg acgccgccgg ccagccggct gcccacggtg
    12421 agcggtgccg attcgatgcg cgaggcgaag ctgccgtctg gcttgcgttc gaccaccagg
    12481 cgctggaagc ggtcgctgtc gtcctgcgcc cagcgggccg tcaggcgcac caggcggtgg
    12541 tcgtccgtgg cctcggccga catggagcgg cccacgcggc ccagcaggtt ctggcgcacg
    12601 gcattgtcgg cacgcaaaaa ggccgcggcg gcggggtcgg caacgcccag gcgctgcagg
    12661 atgctctcgg ccgtgtcagc gccgcgcgtg acgtcggagc ggtacagcga aaagcccggc
    12721 ccgtccacca gggccgacag tggctcgccc tcggccagcg actgcacggc ctgggtcacg
    12781 gtgctcaccg gcagttcggc cggatcgggc gccagcgagg ccacggcaaa ggcgccgccg
    12841 ccggtggtca gcagcagcgt tgtcagggcg gcggtaatgc gtttcggatg ggtttgaagg
    12901 gtctgcttca gctggctgag caaaagcagt ccggcggtgg tcaagccgtt gttcaagagt
    12961 cttaccccag gcagatgtgc ctgcatgccg ctggcgcggg cgcgtgtggg cggcaggcac
    13021 aaggaatttg gtatggctgc ggaaaggggc cccgggccgg tgggatgggc ggggccacta
    13081 gaatccgctg ctacaaagcg ctcgagagta tagtccctgc gcccctggtg acaccctgaa
    13141 aacccttatg aatcaaaccg ttgttacgaa tttcccggtg accgatgacg tacgtcaggc
    13201 attggaagtg tcactgcgcg gcgtcgacga gttgttgccg cgcgaggact ggacgcgcaa
    13261 gctggcgcgc agcgcggcca cgggccagcc gctgcgcatc aagctggggc tggatccgac
    13321 ggcgccggac atccacatcg gccacacggt ggtgctcaac aagatgcgcc agctgcagga
    13381 tctgggccac caggtgattt ttctgatcgg cgacttcacc acgctgatcg gcgacccctc
    13441 ggggcgcaac agcacgcgcc cgccgctcac ggccgagcag atcaaggtca acgccgagac
    13501 ctactacacc caggccgcca aggtgctgga cccggcgcgc accgagatcc gctacaacag
    13561 cgaatggaac gacgccctgg gcacgcgcgg catgatccag ctggcggcca agtacacggt
    13621 ggcgcgcatg atggagcgca acgactttca ccagcgcttc accgagggca gctccatcag
    13681 cctccatgaa ttcttgtacc cgctgctgca gggctatgac tccgtggcgc tcaaggcgga
    13741 cctggagttg ggtggcacgg atcagaagtt caacctgctg atgggccgcc atctgcagca
    13801 ggagtggggg caggagccgc agtgtgtgct caccatgccg ctgctggtgg ggctggacgg
    13861 ggtggagaag atgtccaagt ccaagaacaa ctacatcggc atcaccgagg acgccaacac
    13921 catgttcgcc aaggtgctgt cgatctcgga cgagctgatg tgggactggt acaccctgct
    13981 gtcgttcagg agcctggccg agatcgccag cctcaggcag gaagtggccg aggggcgcaa
    14041 ccccaaggac gccaaggtgc tgctggccaa ggagatcacg gcgcgctttc acagcgccgc
    14101 cgcggccgat gcggctgaag aagacttcaa caaccgcgcc aagggcggca ttcccgacga
    14161 tatccccgat ctgcagctgt gcggcgcgcc gctgggcatt gcggcgctgc tcaagcaggc
    14221 gggcctggcg ccctcgagca gcgaggccaa ccgcctgatc gacggcggcg gcgtgcgcgt
    14281 cgatggcggc gtggtcagcg acaagggcct gaagctggcc accggtcgct acgtggtgca
    14341 ggtgggcaag cgcaagttcg cgcgcgtgac attggcttga tgtggccata ggtcatttgc
    14401 aataaaaacg gcctttgcgc ttgtgcagca agcgctatta gctattaaaa atgtagctag
    14461 ttatggggtg tcaggggtcg tgaccggcgc cgcctcgccg atggcgcggc gcgtggtctc
    14521 ggcctgcagc agcagccagg cgcagaaggc ctggatctcc gggcgctggc cgctcctcgg
    14581 ccccaccacc agccaatagg ccagcggcga atccagccga tggccgggca gcacctccac
    14641 caggtcgccc gatgccaggc tttgcgccac cagcggcatg cgtgccagcg ccaggccctg
    14701 gccggccagg gccgcctggg cgatctgctg ggcgtagttg aagtacagcc agcgccgcgg
    14761 ctgcagcttc tccaggccat gcacctggaa ccagcggcgc caggtgatca gctcgaaata
    14821 gggcaggcgg tgcgcatccc cggcctcgat cagggtgaac cgcgccacgt cggccggctc
    14881 gtgtatcggc ggcatgcttt tgagcagcca ggggctggcg accaccgcca gctgctcgcc
    14941 aaacaggcgc tcggcgcccg ccggctggct gccgggcacg gccgagcgca gtgccaggtc
    15001 cacgtcggcg gtctccagat ccaccatggc atcgctggcg tccatgcgga tgtcgatgtc
    15061 cgggttgtcg cgctggaact cctccatgcg tggtatcagc cacatcgagg caaagctggc
    15121 ccaggtggtg atcgccacgc tcttgcgtcc ggccgtttga cgcaccaggc gcacggcggc
    15181 gtccatgcgc tccagggccg gcgccacggc gcgctgcagc tggccgccgg cgccggtcag
    15241 ctccacggcg cgggtgtggc gcaaaaacag cggcacgccg acctcgtcct ccagcgcctg
    15301 gatctggcgg ctgacggccg actgggtcag ggccagctcc tcggccgcgg cgcgaaagtt
    15361 cagatgccgg gccacggcca gaaaggcgcg ccaatggccc acggccacgg gccgggtgcg
    15421 caggtgggtg atgggtggca ggggcgtttt catggcctgc gatgatgcct gaattgatgc
    15481 gatttgagaa ttgatggtgg ctcttgtttt cattggacga tagcccgtgg caaggcgcaa
    15541 actggcgcca catcaatggt ttcaggagac cgccatgacc gcgtcgcatg ttctgaattc
    15601 gcaacaatgg ggcgcctgcg cagacgctgc cggcgccgat ccgtaccggc tcgccacggg
    15661 tgccgcacgc agcctgcggc cgacgacggc catgcggctg cgcgtggtct gcggccaggc
    15721 ctgggtgacg gtagactatg gcccccatgg ctggcgcgag gactcgggcg acctgctgct
    15781 gcaggccggc caaagcctgc gcatagcccc cggccagcat gcggtggtcg aaccgctggg
    15841 ccagcaggcg ctgcagtacc aatggtgcag cgcgggataa tggcgcgttt gcccggccgg
    15901 cagggctggc cgggcgtcga tcagccaatc ccacttacct catgacagca ctggacatca
    15961 tcatcatggc cgcgggcaag ggcacgcgca tgaaaagccg cacccccaag gtcttgcagc
    16021 gcctggccgg caggccgctg ctgcagcatg tgctggacca ggcggccagc ctgcaggcac
    16081 gacgggtggt ggtgatcacc ggtcatggtg ctgcagaagt agaagctagt tgcgcacaga
    16141 gaacgggcgc tagcggccaa tttgacctta aatgcgtgcg ccaggagccg caactgggca
    16201 cgggccacgc cgtgcagcag gccgtgccgc agctggccgg cgacggcatg gtggtggtgc
    16261 tctcgggcga tgtgccgctg acccaggccg ccacgctggc cgcgctggtc gatgcggccg
    16321 gcggcgaacg gctggcgctg ctgaccgtgc agctgagcga ccccacgggc tatggccgca
    16381 tcgtgcgcgc cgccaacgac caggtgcagc gcatcgtcga gcacaaggac gccagcgccg
    16441 agcagcgcgc cattggcgag atctacagcg gcatcatggc cgtgccggcg cgcctgctcg
    16501 ctggctggct ggcccgcctg accaacgaca acgcccaggg cgagtactac ctgaccgaca
    16561 tcgtcgccat ggccgtggcg gacggcgtgc cggtggtggc gcaccgcatc caggatgcgc
    16621 tgcaggtcgc cggcgtgaac agcccggtgc agctggccga gctggagcgc gcgcaccaga
    16681 tgcgccaggc ggcggcgctg atggagcagg gcgtgcgcct ggccgacccg gcgcgctttg
    16741 acctgcgcga cgatgccaag accggcgcgc gcggcgagct gatttgtgcg caggacgtgg
    16801 acatcgacgt cggctgcatc ttcaccggcc gcgtcgagct gggcgagggc gtgcagatcg
    16861 gcgcctattg ctgcatcgcc aacgccagca ttgccgctgg cgcggtgatc caccccttca
    16921 cccatatcga cggcgagaag gccggcgcca gcgtcggcga gggcgccctg gtcggcccgt
    16981 ttgcccgcct gcgtccgggc gcgcaactgg ggcgcgaggt gcatatcggc aactttgtcg
    17041 aggtcaagaa ctccaccctg gccgacggcg ccaaggccaa ccacctggcc tacctgggcg
    17101 acgccacggt gggcgagcgc gtcaactacg gcgccggctg catcaccgcc aactacgacg
    17161 gcgccaacaa gcaccgcacc gtgatcgagg ccgacgtgca tgtgggcagc aactgcgtgc
    17221 tggtggcgcc cgtcatcctg ggcgccggcg gcaccgtggg cggcggctcc accatcacca
    17281 aggacacgcc ggcaggcgcg ttgagcgtgg cgcgcggcaa gcaggtcaac attgccggct
    17341 ggaagcgccc ggtgaaacag gccaagtgac ggccatgcaa agcgacgcgg ctgccactga
    17401 aacaccagag cagttgcgcg ttgcactcga ccaggcccag cgccagattg ccgacatggc
    17461 cgccgcgcac gaggcctttc tgcgcgccgt ctcgcatgat ctgcgtgccc cgctgcgcca
    17521 tgtgacctcc tacggcgccc tggcgcgcga gttgctgcag gagttgtcgc aggagctgcc
    17581 gggccagccg ccgcagttgc tcgaagccat ggactttctg gccaccatgg agggatcggc
    17641 gcgccgcatg gcgctgatga tcgacggcct gcaggccatc gcccgcgccg gccgtgcccc
    17701 gctgcgcctg caatcgctgg atctggccgg cgccgtgcag caggcgcgcg cggccctggg
    17761 cggtgcggcc gacggcgtgc agtggcagat tgccggcgcc atgccgccgg tgcaggccga
    17821 cgccgagctg ctcacccagc tgctcaccca gctgctggcc aacgccctca agttctcgcg
    17881 cggcgtggcc cagccgcgca tcgcactgca tgccgaggtg gcgcccgccg gtcgcgtgca
    17941 tgtcaccctg caggacaacg gtgtcggctt tgacggcacg cgcgcgcagc agctgttcgg
    18001 cgtgttccag cgcctgcacc gcgaggccga cttcgacggc gtgggcgccg gcctggccct
    18061 gtgccaggcc attgcccagc gccacggcgc cagcatcagc gccaccgccg ctcccggcgc
    18121 gggttgctgc atccgcctgg attggcccgg ggctgcggcg cccggctgct cagggtaggt
    18181 ccacgccctg ttccagctgg cgcaggcgct ggcgcagcac cgccacctct tcgatcaggt
    18241 cggccgtgag cgccgccagc tcggggtcgg cgtcgaagct cgattccagc cgcgccaggc
    18301 ggcgtgcgcg cagcacggtg gaactggtga acagccagcg gccgccggac tgttcccctt
    18361 gcagcaagcc cgcctccagc cgctccacga cccagccggg cgccatgcag cagctgcgcg
    18421 ccagctcctc cagtgtcagg cggttgggct cgtcgagcag ctcggccagg gccctgtcca
    18481 gaaaatggtg cgtgaccatg gtcagcctcc ttgcgcgccc tgcgtggcgc gcggcttgaa
    18541 gtcggggaag gcctgggcca gggcgcggta ggcctgctgc tgcgccgggg tggcggcggc
    18601 gggcagggcc acatgcagct ccaggtacag gtcgccgggc gtggcggcgg gtatgccgcg
    18661 ctctctcagg cgcagcttgc gcccgggctt ccagcgtggt ggcacggtga cctcgacggt
    18721 ggcgccgccg ggtgtctgca cctcgatggc gccgcccagc tcggcctccc agggcgccag
    18781 ggtgacggct tgatagacgt cgcgatcctc ggcgcgccag cgggtgtcgg gcttgaaccg
    18841 cacctccagg aacaggtcgc ccgccggcgt gcctgggccg ccctggccgg acaggcgtat
    18901 cagctggccc gcgcgcacgc ccttgggaat ggttacctgc agctggcgct gttcgttgac
    18961 cagatgcccg gccgcgtcca gccgcgcgcc gtgcagggcc agcagctgct cgccgccgtg
    19021 gtaggcgtcg cgcagatcca gctcgatgga cgcatggtga tcgcgcccgc gctgcggtgg
    19081 cggcgcgcct cgggcctggg cgccgccgcg gccctgggca tgctgggcgc gggcggcgcg
    19141 gccgaagagc tgctcgaaga agtcgctgaa ttcggcgccg tccaggccct cggcgccggg
    19201 tgcgccggtg aactcgaaac cggcgtccca gttgggcggc gggcggaagt cctgacccgg
    19261 gtggtgcgcg gcctcttgcc ccaggcggtc gtaggcagcg cgcttttcgg ggtcggacag
    19321 gacggtgttg gcctcgttca cctcggccat gcgggcagag gcgtcagcct ccttgctgac
    19381 gtcggggtgg tagcggcgcg ccagcttgcg gtaggccttc ttgatgtcgt ccgcaccggc
    19441 atcgcgcggc acgcccagga tttggtagta gtccttgaac tccatgcatc ctccatgctt
    19501 ggcatgggtc gagggtggcg cctatgcggc cacaggtcaa ggggcggagt tgtttttact
    19561 atcgtatttg tagcttgtag cgcttattgg gtatgcgcta tcggcaattt tgtctcgaat
    19621 tttgcgcgct gcgtggcgaa gaagaccttg acgttgcgca cattcgcgtc ctgggtgaac
    19681 aggcgctgcg acagggccag gtaggccggc atgtcgcgcg tgtgcacgac gagcacgaag
    19741 tccggcccgg gcgacacgcg ccagcattgc tgcaccgcat cgtcggcgct ggcgcgggcc
    19801 tggaaggcgt ccaggtgctc ggcgccctgg cggtccagtg acacctcgac aatggccgcc
    19861 agcccgtggc cttgcagcgc cgccagccgg tcgggctgca gcagcgccac ctggcgctcg
    19921 atcaggccgg actcctgcag ccgccgcacg cggcgcaggc aggtgggggg cgaaacatgc
    19981 acgcgctcgg ccaaggcttg gttgctcagc gaggcatcgc gctggagcca gtccagcagc
    20041 tgccagtcgg ttgcatcggg tatgaaatca gattccatta caaataaaaa tgagaaataa
    20101 aatttgtcat ttcattgtca atgaaattat tgcgcataaa caaaagaaaa aatcttgaaa
    20161 atttctaact tgtcagtcta gcatcgcatc cattgttcat ttggggaggt ttttctatgt
    20221 gcggcatcgt cggcgcggta tccacgcgca acatcgttcc catcctggtg cagggcctgc
    20281 agcggctcga gtaccgcggc tatgactcct gcggtgtggc cgtgcatgag gccagcatgg
    20341 gtcaacaccc cgcaggcggc ctgcggcgcg cgcgcagcac ggcgcgcgtg gccgagctgc
    20401 tggagcaggt ggcgcaggac gacctgcagg gcgccaccgg catcgcccac acgcgctggg
    20461 ccacgcatgg tgcgcccgcg gtgcacaacg cccacccgca tttcagccac ggttgtggcg
    20521 aggcgccggc cgagacggcg cgcgccggcc gcgtggccct ggtgcacaac ggcatcatcg
    20581 aaaaccatga gcagctgcgc gcgcggctgc aggcgcgcgg ctatgtgttc tccagccaga
    20641 ccgataccga ggtcatcgcg cacctggtgg acagcctgta cgacggcgac ctgttccagg
    20701 ccgtgcgcgc cgccaccgcc gagctgcatg gtgcgtttgc cattgccgtg atccacaagg
    20761 acgagccgca gcgcgtggtc ggcgcgcgcg ccggctcgcc cctggtcctg ggcgtgggcc
    20821 agggcgagaa ttttctcgcc agcgacgcca tggccctggc cggcgtgacc gatcagatcg
    20881 tctacctgga ggagggcgac ctggtggatc tgcagctggg ccgctactgg atcaccgggc
    20941 cggatggcca gcccctggcc gaaggcagcc gccccgtgcg caccgtgcac gcgcacagcg
    21001 gcgcggccga gctggggccg taccgccact acatgcaaaa ggaaatcttc gagcagccgc
    21061 gcgccattgc cgacacgctg gacggcgtcc aggccatcgt gcccgagctg tttgacggcg
    21121 ccggccagca tggccagccg ggccaggccg cctggcgcgt gttcaaggag atcgactcgg
    21181 tgctcatcct ggcctgcggc accagctact acagcggctg cacggccaag tactggctcg
    21241 aatccatcgc cggcattccc acgcaggtgg aggtggccag cgaataccgc taccgcacca
    21301 gcgtgcccaa cccgcgcacc ctggtggtca ccatcagcca gtccggcgag acggccgaca
    21361 ccctggccgc gctgcgccac gcccagggcc tgggcatgaa gcacacgctg accatctgca
    21421 atgtggccac cagcgccatg gtgcgcgaat gcgcgttgac ctacgtcacg cgcgccgggg
    21481 tggagatcgg cgtggcatcc accaaggcct tcaccacgca gctggcgggc ctgttcctgc
    21541 tgaccctggc gctggcgcaa accaaggggc gcctgacaga ggaaaaggaa gcccagtacc
    21601 tgaccgccat gcgccatctg ccggcggccc tgcaggccgt gctggcgctg gagccgcaga
    21661 tcatcagctg ggccgaggac ttcgcgaaga tggaaaacgc gctgttcctg ggacgtggca
    21721 tccactatcc ggtggcgctg gaaggggccc tgaaactcaa ggaaatcagc tacatccacg
    21781 ccgaggccta tccggccggc gagctcaagc acgggcccct ggcactggtg acgagcagca
    21841 tgccggtggt caccgtggcg cccaacgacg agctgctgga aaagctcaag agcaacatgc
    21901 aggaagtgcg cgcgcgcggc ggcgtgctgt a
//
LOCUS       DAOMTQ010000033        21869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_276_length_21869_cov_26.204456, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000033 DAOMTQ010000000
VERSION     DAOMTQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 21869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21869
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_276_length_21869_cov_26.204456"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PK518_05150"
     CDS             complement(<1..844)
                     /locus_tag="PK518_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRM48333.1"
                     /translation="MTVIAKNDSKTQRMLWVQALQAVRAHLQARGAHPARCMVLLPYA
                     QLMGEAQRQWALLCSEGFAPRFETTRNWAQRLIGFMPQGLDYAQDQARDRFTGAALLE
                     KAGLAAQREMLLPALLDAAAQLAPLAAAVPPEQRSAWLEQQRAVVGLAGEGSALRYEA
                     AVARLALEWVAASRHATDVLFTPAVRAEVDAIVLLQGFQPDALATSLCAAWGEAALVL
                     LLWRADSPPLPLGEGWGEGGSPHIARHPSQDAEDEAQRAAACVIAHVQAGRTPVALAS
                     TDRAL"
     gene            947..1279
                     /gene="trxA"
                     /locus_tag="PK518_05155"
     CDS             947..1279
                     /gene="trxA"
                     /locus_tag="PK518_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HRM48334.1"
                     /translation="MASDLIKQLSDASFEADVLKSSTPVLVDYWAEWCGPCKMIAPIL
                     DDVAGAYQGKLTVAKMNVDENREIPAKFGIRGIPTLMLFKDGQLAATKVGAMSKAQLT
                     AFIDQQLA"
     gene            1499..2761
                     /gene="rho"
                     /locus_tag="PK518_05160"
     CDS             1499..2761
                     /gene="rho"
                     /locus_tag="PK518_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRM48335.1"
                     /translation="MHLNELKALHVSEVLKQAEALEIENGGRMRKQELMFAIIKKRAK
                     AGEQVFADGVLEILPDGFGFLRSPDSSYTASTDDIYISPSQVRRFNLHTGDMIEGEVR
                     IPKDGERYFALTKLDTVNGGPPEQNKHKVMFENLTPLFPKEQMRLERDIKGEENITGR
                     IIDIIAPIGRGQRALIVAPPKSGKTVMMQHIAHAITANNPDVHLMVLLVDERPEEVTE
                     MQRSVKGEIIASTFDEPAARHVHVAEMVIERAKRLVELKKDVVILLDSITRLARAYNN
                     VVPSSGKVLSGGVDSNALQRPKRFFGAARKVEEGGSLTIIATALVDTGSRMDEVIFEE
                     FKGTGNCEIHLSRRLYEKRVFPAIELSKSGTRREELLLAPEILQKTRILRQFMYNMDE
                     IESMELMIKKMRETKSNVDFFEAMRRGG"
     gene            complement(2819..3631)
                     /locus_tag="PK518_05165"
     CDS             complement(2819..3631)
                     /locus_tag="PK518_05165"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRM48336.1"
                     /translation="MELSTLRVTLDNHVATVRLNRADKANAMNAAMWQEIRQAFDWVD
                     TTAAVRVAVLQAEGKLFCSGIDLAMMMQMPAQIADDCEGRQREKLRRVILDLQDSLTS
                     LERCRKPVLAAIHGACIGGGVDLVVCADMRYASSDASFAIKEIDIGMVADVGTLQRLP
                     KLVGQGITRELAYTGRKLGAAEAQSIGLVNRVFDSREALYAGVHEIAAQIAAKSPLSI
                     RGSKEMLNYARDHSVADGLNHVATWNAAMLQSDDLMAAMMAGMSKQQPQFKD"
     gene            complement(3716..5737)
                     /locus_tag="PK518_05170"
                     /pseudo
     CDS             complement(3716..5737)
                     /locus_tag="PK518_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
     gene            5854..6387
                     /locus_tag="PK518_05175"
     CDS             5854..6387
                     /locus_tag="PK518_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRM48337.1"
                     /translation="MSLPHAVLTSLLEKSTSGYDLARRFDKSIGYFWHATHQQIYREL
                     ARMEGAGWIDGFVPPDAGKTRKKHYRVLPAGRAELVRWASEPTAPVDLRDGFTVKLRA
                     DAVLDEVDLSAELKRSLARHEERLAQYRQIEARDFPPNQARTRPQTLQYMILKKGILY
                     EEAELAWGREMLALLQP"
     gene            6508..6753
                     /locus_tag="PK518_05180"
     CDS             6508..6753
                     /locus_tag="PK518_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRM48338.1"
                     /translation="MKEGIHPNYREVLFVDLSNGFKFVTRSCVNTKESETFEGKEYPL
                     YKLDTSSESHPFYTGTQKSVDNMGGRVERFRNRFGKK"
     gene            6847..8544
                     /locus_tag="PK518_05185"
     CDS             6847..8544
                     /locus_tag="PK518_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48339.1"
                     /translation="MNHPTPAIVAQSAVRPLPRWALLLLCLAYAVPGFVGRAPWRSDD
                     MASFGYMRELALGHTSWLHPLLAGLPGDGDGLLHYWLGAWAIQALGGWLPLDLAARVP
                     FIALLLLTLSATWYGVYYLARGPGAQPVAFAFGGEAQPADYARAMADGGLLALIACLG
                     LAQLSHETTGYLTQLASTALLFYAGAALPWRMAWPLLAGLIGLLGLVLSGAPSIAVLL
                     GLGCAWLVWHSHGPTLRTRATALALMLAVTLLAALLAQQLDLWRWRIVRVDGPWEWQS
                     LVRLLTWFSWPAWPLALWTLWRWRQLIASRHWHRHLWLPLWFALVTLGATLATRPANR
                     ALLLSLPALAALAAFALPTLKRSIAALIDWFTLLFFSISAIAIWVVWLSVQTGWPAKP
                     AANVAKLAPGYVSTFSVLPFAVALVATLAWCLLVAWRTARNRPAIWKSLVLPAGGATL
                     GWLLLMTLWLPILDYGRSLAPQVAGVRAVITERQGCVAAYGLSRAQVAALMYHGELPM
                     VDLPRVAQCDWLVADPDASPSVVTLLPPGQWVEVAIVSRPTDRNDRLLVLHRRDDGR"
     gene            8544..9911
                     /locus_tag="PK518_05190"
     CDS             8544..9911
                     /locus_tag="PK518_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM48340.1"
                     /translation="MSELSTISRHALTVLAGQLAVMAFGVTDTIVAGRYAQEALAALS
                     IGSAVFISVYVGLMGVLQALMPVWAEQRGANAQAAIGRSVRQSLYLWAVATALGMAVL
                     LSPEPILRWTEVPPELRGAVRAYLAILAFGLPPALLFRIYSTLNQALGHPQLVTWLQV
                     GSLALKLPLSIWFTFGGAGLAPQGVAGCAWATLVVNYGLMLLGWAMLRRHAMYAPLQL
                     WRALERPDGTQIARFLRLGLPAGLAILVEVTSFTLMALFIARQGALASAAHQIAANLA
                     AVVYMVPLSLAIATSARVSYWRGAGDERQAGATALNGFWLAALMGCALAATLVIAKQP
                     IAGLYSTSPEVVALAASLLVWVAAYQVADALQTLCIFVLRSYHITLAPLVVYGVLLWG
                     VGLHGGYRLAYQGWAGWGPYHSPAPFWAASAAALAVTALVFAALLLGVLRLSRRKADE
                     RQVHA"
     gene            complement(9950..11284)
                     /gene="glmM"
                     /locus_tag="PK518_05195"
     CDS             complement(9950..11284)
                     /gene="glmM"
                     /locus_tag="PK518_05195"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRM48341.1"
                     /translation="MTRQYFGTDGIRGTVGQAPITPDFVLRLAHAVGRVLRRTEERPT
                     VLIGKDTRISGYMLEAALESGFNSAGVDVVLLGPLPTPGVAYLTRAQRASLGVVISAS
                     HNPYPDNGIKFFSAQGTKLPDAWEEEVEAALQQPPMWADSASLGKTRRLDDAAGRYIE
                     FCKSTFDHDLSLRGVKIVVDAAHGAAYQIAPMVFHELGADVVAIGCAPDGVNINHEVG
                     ATHPEALVRAVCANQADFGVALDGDADRLQIVDASGRLYNGDELLYLMAAERLARDEH
                     VPGVVGTLMTNMAVEQALTRRGVKFVRAKVGDRYVLEELQRQNWLLGGEGSGHLLALD
                     CHTTGDGLISALQVLQACVRSQKTLAELLADVPLFPQVLLNVRLKPDQDWKANAALAD
                     TTREVQAELADNGRLLVRASGTEPLLRVMVEARDADQASRCAQRLADAARAA"
     gene            complement(11304..12152)
                     /gene="folP"
                     /locus_tag="PK518_05200"
     CDS             complement(11304..12152)
                     /gene="folP"
                     /locus_tag="PK518_05200"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRM48342.1"
                     /translation="MLWQTARFDLDLTHARVMGIVNVTPDSFSDGGQHAGTAAALRHC
                     EQLLKEGADILDIGGESTRPGSPALPLQDELQRVLPVMREAVRLGVPISVDTYKPQVM
                     QAALDLGADIVNDIWALRQSGAAQVVAAHPRCGLCLMHMHRDPQTMQTAPMQGDVVPQ
                     VLTFLELQAQYLQGLGVNKSRIAIDPGIGFGKTVQQNFALLARQRELLALGYPVLAGW
                     SRKSSLGAVTGLPVQERLGASVAAALLAVQQGARIVRVHDVRDTVGALATWRAMQEQT
                     PIEQTH"
     gene            complement(12217..14139)
                     /gene="ftsH"
                     /locus_tag="PK518_05205"
     CDS             complement(12217..14139)
                     /gene="ftsH"
                     /locus_tag="PK518_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM48343.1"
                     /translation="MNNQWFSKVAVWLVIAMVLFTVFKQFDTRTGVGAGAVGYSEFLE
                     DVRNHRIKSATIQEGQGGSTEIVAITNDDRRIRTTATYLDRGLIGDLVNNNVKFDVRP
                     REEGSLLMTLLVSWGPMLLLIGVWVYFMRQMQGGGKGGAFSFGKSKARMLDENNNTVT
                     FADVAGCDEAKEEVKEVVDFLKDPQKFQKLGGRIPRGLLLVGPPGTGKTLLAKSIAGE
                     AKVPFFSISGSDFVEMFVGVGAARVRDMFENAKKNAPCIIFIDEIDAVGRQRGAGLGG
                     GNDEREQTLNQMLVEMDGFETNLGVIVVAATNRPDILDAALLRPGRFDRQVYVTLPDI
                     RGREQILNVHMRKIPVGQDVNPAVIARGTPGMSGADLANLCNEAALMAARRNARTVEM
                     QDFEKAKDKIIMGPERKSMVMPEEERRNTAYHEAGHALIGKLLPKCDPVHKVTIIPRG
                     RALGVTMSLPEKDRYSYDKEYMLNQIAMLFGGRIAEEVFMHQMTTGASNDFERATSIA
                     RDMVMRYGMTAALGPMVYAENEGEVFLGRSVTKTTNISEETMQKVDAEVRRIIDEQYA
                     LARKLIEDNQDKMHAMAKAMLEWETIDAEQLDDIMAGKEPRPPKDWTPRTPPSGGDGS
                     GGGTPAVKPDPTPTAA"
     gene            complement(14277..14939)
                     /locus_tag="PK518_05210"
     CDS             complement(14277..14939)
                     /locus_tag="PK518_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRM48344.1"
                     /translation="MKVQTKSKKVNKAWLNDHVHDPYVKLAQKEGYRARAAYKLKEID
                     EQFGLIQPGHTVVDLGSAPGAWSQYVRRRLSPGGAAVGQLAGTIIALDILPMEPIEGV
                     VFLQGDFRDDEVLARLQQAVQGRPVDLVVSDMAPNLSGVESVDAVRIAHLVELAVDFA
                     VHHLKPDGALVVKLFHGSGYAQLVQLFKDTFRVVKPVKPKASRDRSSETFLVGIGLKP
                     TA"
     gene            14960..15430
                     /locus_tag="PK518_05215"
     CDS             14960..15430
                     /locus_tag="PK518_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HRM48345.1"
                     /translation="MPQIQLTPAERREHRANAHHLDPVVMIGGDGLTPAVQKEIDAAL
                     NAHGLIKVRVFGDDRAAREAMYQQLAGELNAAPIQHIGKLLVLWRPIPAKDKPLDEDR
                     KSGPRDVKVLKYGRPGQRPEVKQLRVLGNQRLTSGGQIKRAKPKLKSVKKNQGQ"
     gene            15430..16167
                     /locus_tag="PK518_05220"
     CDS             15430..16167
                     /locus_tag="PK518_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM48346.1"
                     /translation="MTSPRQRHILCMKWGTKYGPEYVNRLYAMVRRHLSGDFNFVCLT
                     DDSAGIRPEVQCLPIPPLNLPAGIPERGWNKLATFTGDLYGLKGTALFLDVDVVITGS
                     LDDFFTQPGDFLIIHDYKRPWRITGNSSVYRFELGAHPDVLAYFRGHFEEIRRQYRNE
                     QAYLSDFLHKQGKLQYWPKDWCPSFKYHGIPPWPTNYWKAPFVPEGARIVIFHGECNP
                     PDALAGRRNRRFRHIQPATWVAEHWRE"
     gene            complement(16173..16580)
                     /locus_tag="PK518_05225"
     CDS             complement(16173..16580)
                     /locus_tag="PK518_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HRM48347.1"
                     /translation="MRERLPVLAAALWWGSLCAMGFVAVPLLFAHLPTPALAGNMAAR
                     LFEAQTYISIACCALLLVISKHKHAEKQEPWAQAAMVFVIAGLLLALLQQYGVAPRIL
                     ARQNLRLWHGAGSAMYALQWLCALCVLWRTARR"
     gene            complement(16594..17070)
                     /gene="greA"
                     /locus_tag="PK518_05230"
     CDS             complement(16594..17070)
                     /gene="greA"
                     /locus_tag="PK518_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRM48348.1"
                     /translation="MATIPITKRGAEKLKAELHRLKTVERPAVINAIAEARAQGDLSE
                     NAEYEAAKDRQGFIEGRIMEVEGKLSAAQIIDPSQLDAGGRVVFGATVELEDEDSGDT
                     VKYQIVGEDEADLKHGLINVSSPIARALIGKEEGDTAEVQAPGGLRRYEVVTVSYL"
     gene            complement(17172..20420)
                     /gene="carB"
                     /locus_tag="PK518_05235"
     CDS             complement(17172..20420)
                     /gene="carB"
                     /locus_tag="PK518_05235"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM48349.1"
                     /translation="MPKRTDIQSILIIGAGPIIIGQACEFDYSGVQACKALREEGYRV
                     ILINSNPATIMTDPATADVTYIEPITWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     WRNGVLNKYRVELIGAKPEAIDKAEDRLKFKDAMTRIGLASARSGIAHSMDEAWAVQK
                     NVGFPTVIRPSFTLGGTGGGIAYNPEEFETICKRGLEASPTNELLIEESLLGWKEYEM
                     EVVRDRADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDREYQILRDASIAVLREIG
                     VDTGGSNVQFAVNPKDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGATPASFEPSIDYVVTKIPRFAFEKFPTADSRLTTQMKSVGEVMAMGRTFQ
                     ESFQKALRGLEVGVDGMNEKTQDREILEKELGEPGPERIWYVGDAFAQGMSVDEVFDL
                     TKIDRWFLVQIEEIVKIELDLDKLYADKGDQALALLDAATLRALKQKGFSDRRLAKLL
                     HTTEKAVREARRALNVRPVYKRVDTCAAEFPTNTAYMYSSYDEECEAAPTTKKKIMVL
                     GGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTL
                     EDVLEIVDKEKPEGVIVQYGGQTPLKLALGLEREGVPIIGTTPDMIDAAEDRERFQKL
                     LNDLGLRQPPNATARTEAEALEKAAALGYPLVVRPSYVLGGRAMEIVHEQRDLERYMR
                     EAVKVSNDSPVLLDRFLSNAIECDVDCVRDATGAVFIGGVMEHIEQAGVHSGDSACSL
                     PPYYLKADTVAEIKRQTTAMAHGLNVVGLMNVQFAIQETDAGDVIYVLEVNPRASRTV
                     PFVSKATGIQLAKVAARCMAGQSLASQGVTREVTPPYFSVKEAVFPFVKFPGVDTILG
                     PEMKSTGEVMGVGKTFGEAFVKSQLGASTRLPTGGKVFLTVKNADKGRAVAIARDLSA
                     MGFELLATKGTAAAIAEAGVAVQVVNKVTEGRPHIVDMIKNDEIAMVINTVEERRNAI
                     ADSRAIRTSSLQSRVTTFTTIFGAEAAVEGMKHLDKLDVYSVQELHAQLPTA"
     gene            complement(20420..21598)
                     /gene="carA"
                     /locus_tag="PK518_05240"
     CDS             complement(20420..21598)
                     /gene="carA"
                     /locus_tag="PK518_05240"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRM48350.1"
                     /translation="MLSSLKAAFPPAILALADGTVFIGNSIGSVGTTVGEVVFNTAMT
                     GYQEILTDPSYCQQIVTLTYPHIGNYGVNEEDMESSKVQAAGLVIRDLPLIASNFRAQ
                     QNLSQFLNAQGAVAIANIDTRKLTRLLREKGTQNGAIVGLAASEAVTPERIAAAIAAA
                     KNAPSMAGLDLARVVSTQQPYAWEQTEWELGQGFGKLDKPRFHVVAYDFGVKRNILRM
                     LAERGCKLTVVPAQTSAEEALALQPDGIFLSNGPGDPQPCSYAIEAARTFLDQGIPTF
                     GICLGHQIMALALGAQTFKMPHGHHGGNHPVKTLASGHVCITSQNHGFAVDAATLPSS
                     ARATHVSLFDGTLQGLECTDKPAFSFQGHPEAAPGPNDIGHLFDRFMASMAAAGQEKK
                     "
BASE COUNT         3574 a         7262 c         7066 g         3967 t
ORIGIN      
        1 ccagggcgcg gtcggtgctg gccagggcca ccggggtgcg gcctgcctgc acatgggcga
       61 tgacgcaggc ggcggcgcgc tgggcttcgt cttcggcgtc ttgcgacggg tgccgggcga
      121 tgtggggact gccgccctca ccccagccct ctcccagggg gagagggggg gagtccgccc
      181 gccacaacag cagcaccagg gcggcttcgc cccaggcggc gcacaggctg gtggccagcg
      241 catcgggctg aaaaccttgc agcaggacga tggcgtcgac ctcggcgcgc acggcggggg
      301 tgaataacac gtcggtggcg tgacgcgagg cggccaccca ttccagcgcc aggcgcgcca
      361 cggcggcctc gtagcgcagg gcgctgccct cccccgccag gccaaccacg gcgcgctgct
      421 gctccagcca cgcgctgcgc tgctcgggcg gcacggcggc agccaaaggg gcgagctgcg
      481 cggcggcgtc gagcagcgcc ggcagcagca tttcgcgctg ggcggccagg cccgcctttt
      541 ccagcaacgc cgcaccggtg aagcggtcgc gcgcctggtc ctgggcgtag tccaggcctt
      601 gcggcatgaa gccgatcagg cgctgggccc agttgcgcgt ggtctcaaag cgcggggcaa
      661 agccttcaga gcacaacaac gcccattggc gctgggcctc gcccatcagc tgtgcgtagg
      721 gcagcagcac catgcagcgc gccgggtggg cgccacgcgc ctgcaggtgc gcgcgcaccg
      781 cctgcaaagc ctgcacccac agcatgcgct gcgttttgct atcgtttttc gctatgacag
      841 tcatagcgtc tggcgcgccg ggcataccgg cagtcggggg attggtttct gtcacaatgg
      901 ccctaattta acctctgtcc aatcccaaaa ttcccaagga aagcccatgg ccagcgacct
      961 catcaagcaa ctctccgacg ccagcttcga agccgacgtg ctcaagtcca gcacccccgt
     1021 gctggtcgac tactgggccg agtggtgcgg cccctgcaag atgatcgccc ccatcctcga
     1081 cgatgtggcc ggtgcctacc agggcaagct caccgtggcc aagatgaacg tggacgagaa
     1141 ccgcgagatc cccgccaagt tcggcattcg cggcattccc acgctgatgc tgttcaagga
     1201 cggccagctg gccgccacca aggttggcgc catgagtaag gcacagctca ccgctttcat
     1261 cgaccaacaa ctcgcctgat gaaccctgcg ccggccagcc ctgcgcgggc ctggccggtg
     1321 ccgtgccaaa agcacaaaaa tttttcctgt cataattcgc gccgaccgtc tcgccgcaca
     1381 tatcgaccag cggccagtct caggtcaccg gctcgccagg gcaacccaag gcccatacgg
     1441 cacgcagcct cttcccctcc agtcacagac atatctactc cgcgtcagga gtcatttcat
     1501 gcaccttaac gaactcaagg cactgcacgt gtccgaggtc ctcaagcagg ccgaagccct
     1561 ggaaatcgaa aacggcggcc gcatgcgcaa gcaggagctg atgttcgcca tcatcaagaa
     1621 gcgcgccaag gcgggcgagc aggtgtttgc cgacggtgtg ctggagatcc tgcccgacgg
     1681 tttcggcttt ctgcgcagcc ccgacagcag ctacacggcc agtaccgacg acatctacat
     1741 cagccccagc caggtgcgcc gcttcaacct gcacaccggc gacatgatcg agggcgaggt
     1801 gcgcatcccc aaggatggcg agcgctactt tgccttgacc aagctcgaca ccgtcaacgg
     1861 cgggccaccc gaacagaata agcacaaggt gatgttcgag aacctgacgc cgctgttccc
     1921 caaggagcag atgcgcctgg agcgcgacat caagggcgag gagaacatca ccggccgcat
     1981 catcgacatc atcgccccca ttggccgcgg ccagcgcgcg ctgatcgtcg cgcccccgaa
     2041 gagcggcaag acggtgatga tgcagcacat cgcccacgcc atcaccgcca acaaccccga
     2101 cgtgcacctg atggtgctgc tggtggacga gcgcccggaa gaagtgaccg agatgcagcg
     2161 ctcggtcaag ggcgagatca ttgcctcgac cttcgacgag ccggccgcgc gccacgtgca
     2221 tgtggccgag atggtgatcg agcgcgccaa gcgcctggtg gagctgaaaa aagacgtggt
     2281 catcctgctg gactccatca cccgcctggc acgcgcctac aacaacgtcg tgcccagctc
     2341 gggcaaggtg ctgtcgggcg gtgtcgattc caacgcactg cagcgcccca agcgcttctt
     2401 cggcgcggcg cgcaaggtcg aggaaggcgg ttcgctgacc atcatcgcca ccgcgctggt
     2461 cgataccggc agccgcatgg acgaggtgat ctttgaagaa ttcaagggca cgggcaactg
     2521 cgaaatccac ctgagccgcc gtctgtacga aaagcgcgtg ttcccggcga tcgaactgtc
     2581 caagagcggc acgcgccgcg aggagctgct gctggcgccc gagatcctgc aaaaaacccg
     2641 catcctgcgc cagttcatgt acaacatgga cgagatcgag tccatggagc tgatgatcaa
     2701 gaagatgcgc gagaccaaga gcaacgtcga cttcttcgag gccatgcgcc gcggcggttg
     2761 accacccagc cccccgcccc acaacgcccc gcaaaattgc ggggcttttt tttgcgggtc
     2821 agtccttgaa ctgcggctgc tgcttgctca tgcccgccat catggcggcc atcaggtcgt
     2881 cgctttgcag catggcggcg ttccaggtgg cgacatggtt caggccgtcg gccacgctgt
     2941 ggtcgcgggc gtagttgagc atctccttgc tgccgcggat cgacagcggc gacttggcgg
     3001 cgatctgcgc ggcgatctcg tgcacgccgg cgtacagcgc ctcacggctg tcgaagacgc
     3061 gattgaccaa gcctatgctc tgcgcctcag ccgcgcccag cttgcgcccg gtgtaggcca
     3121 gctcgcgggt gatgccctgg cccaccagct tgggcaggcg ctgcagcgtg cccacgtcgg
     3181 ccaccatgcc gatgtcgatt tccttgatgg caaagctggc gtcgctgctg gcgtagcgca
     3241 tgtcggcgca gaccaccaga tcgaccccgc cgccgatgca ggcgccgtgg atggcggcga
     3301 gcaccggttt gcggcagcgc tccaggctgg tcaggctgtc ttgcaggtcg aggatgacgc
     3361 ggcgcagctt ctcgcgctgg cggccttcgc agtcgtcggc gatctgcgcg ggcatctgca
     3421 tcatcatcgc caggtcgatg ccggagcaaa acagcttgcc ctcggcctgc agcacggcca
     3481 cgcgcaccgc cgccgtggtg tccacccaat cgaatgcctg gcggatctcc tgccacatgg
     3541 cggcattcat ggcgttggcc ttgtcagcac ggttcaggcg cacggtggcg acgtggttgt
     3601 ccagcgtcac cctcagcgtg ctcagttcca aggccgtgtt tttggcatca ttttggccta
     3661 tagcgcttgt tggtattgcg ctagtagcta ctgtttttgt agtatccgat gtatccaggc
     3721 gcagcgccag ctccgtgccc tgcttgatgg cgcgcttggc gtccagttcc gccgccttgt
     3781 ccgcgccgcc tatcaggtgc acggccacgc cctgggcctg cagcaccgcc tgcagctcgc
     3841 ggttggggtc ctggccggcg cagacgatga cgttgtccac ctccagcagc ctgtcctgcc
     3901 cgtccacggt aatgtgcaga cccgcgtcgt caatcttgcg gtaggtgacg cccgcgagca
     3961 tgtgaacacc ccggttcttc aggcccgtgc ggtggatcca gccggtggtc ttgcccaggc
     4021 cctcgcccac cttgctgctc ttgcgctgca gcaggtgaac ttggcgcggg acatggggaa
     4081 tgtgcgccgc ctgcaggccg ccgcgcgtgg cgccggtggt ctccacgccc cattcggcaa
     4141 agaatttggc cttgttcagg ctggcgctct cgccctcgtg cagcaagtat tcggacacgt
     4201 caaagccgat gccgccggcg ccgatcacgg ccaccttctt gcccacgggt ttcttgtcct
     4261 tgagcacatc gaggtagccc agcaccttgg ggtgagcgat accgtcgatc tcgggcaggc
     4321 gcggcgtgat gccggtggcc agcaccacct gtttgaagcc ttccttcacc agcgtatcgg
     4381 catccacgcg ctggccgagc ttgacgctca cgccggtttg ctggagctgc tcgccaaagt
     4441 aacgcagggt ctcatggaac tcctccttgc ccggaatctg cttggccaca ttgaactggc
     4501 cgccaatttc actgcctgcg tcgaacaggg tcacctccat gccgcgcttg gccgcctcgg
     4561 tggcaaaggc caggccggca gggccggcgc ccaccactgc cacgcgctct tttttcgata
     4621 gcggcagcgg caccaggatg gtttcgtggc aggcgcgggg attgaccagg cagctggtga
     4681 tcttgccggc gaaggtgtga tccaaacaag cctggttgca ggcgatgcag gtgttgatgt
     4741 cctgcgcccg gccagcggcg gctttgttca caaactccgg gtcggccagg aagggccgcg
     4801 ccatgctcac catgtcggcc tggccgctgg ccagaatgcc ctcggccacg tccggcgtgt
     4861 tgatgcggtt ggtggcgacc aggggaatgc cgaccttgcc cttgagctgc tcggtcaccc
     4921 aggcccaggc ggcgcgtggc accttggtgg caatcgtggg gatacgcgcc tcgtgccagc
     4981 cgatgccggt gttcaaaatc gtcacgccgg cggcctccag cgcctgcgcc agctgcacca
     5041 cctcttcatg cgtggagccg ccctcgacca gatcgagcat agacaggcgg aagatgagga
     5101 tgaagttcgc ccccacccgc tcgcgcgtac ggcgcacgat ctgcagcggg aagcggatgc
     5161 ggttctcgta gctgccgccg tactcgtcgt cccgctgatt agtctgcgcg gcgatgaact
     5221 cattgatgag gtagccctcc gagcccatga tctccacccc gtcgtagccc gcgctttgcg
     5281 ccagcgcggc ggcattggcg tagtcctcca ccgtctgctt gacctcctcg gtcgtcaacg
     5341 ccttgggcgg gaatgggctg atgggggcct tgaggctgct gggcgccacc agctccgggt
     5401 ggtaggcgta gcggccaaag tgcaggatct gcatgcaaat cttgccgccg gcggcgtgca
     5461 cggcctcggt caccacccgg tgcttgtcgg cctccgcctg cgtcgtcatg gccgcgccgc
     5521 caggcatggg gcggccctgc tcgttgggcg caatgccgcc ggtcacgatc agcccgacgc
     5581 cgcctttggc gcgctcggcg tagaaggcgg ccatgcgctc aaaaccgttg ggagcttctt
     5641 ccaggcccac atgcatggag cccatgagaa cgcggttttt caaggtggta aagcccaggt
     5701 cgagggggct gagcaggtgc ggataggcag aggtcatggg gcttccttgt cgatggaacg
     5761 gtcatctatg caaccggttg cataaaagtg tacggtcgtt cgtttatcta tgcaactggt
     5821 tgcatagcag attgcccctg gaataatcgg cgcatgtctc tgccccatgc cgtgctcacc
     5881 tcgcttttgg aaaagtccac ctcgggctac gacctggcac ggcgttttga caagtccatc
     5941 ggctatttct ggcacgccac acaccagcag atctatcgtg agctggcgcg catggaaggc
     6001 gcaggctgga tcgatggctt tgtaccgccc gatgccggca agacgcgcaa gaagcactac
     6061 cgcgtgctgc ccgccgggcg cgccgagctg gtgcgctggg ccagcgaacc cacggctccc
     6121 gtcgatttgc gcgacggctt caccgtcaag ctgcgcgccg acgcggtgct ggacgaggtc
     6181 gatctgagcg cggagctcaa acgcagcctg gcccggcacg aagagcgcct ggcgcagtac
     6241 cgccagatag aagcgcgcga cttcccgccc aaccaggcac gcacccggcc ccagacgctg
     6301 cagtacatga ttttgaagaa aggcattctc tacgaggagg ccgaactggc ctgggggcgc
     6361 gagatgctgg ccctgctgca gccctgatgg caattggcga agtcatgcca taatcgaggg
     6421 ctttttgcgg aaagtacgcc gagtttttga atgcgcaatg ggtttgtgca tggccggcac
     6481 ggctcccgca gccatgaaag agttagcatg aaagaaggca ttcaccccaa ctatcgcgaa
     6541 gtgctgttcg ttgacctgtc caacggcttc aagttcgtga cccgttcctg cgtgaacacc
     6601 aaggaatccg agaccttcga aggcaaggaa tacccgctgt acaagctgga cacttcctcc
     6661 gagtcgcacc ccttctacac cggcacgcaa aaatccgtgg acaacatggg tggccgcgtg
     6721 gagcgcttcc gcaaccgttt tggcaagaag taaacggcca ggcccgtccc gggcctgtga
     6781 caagccatca aaggcagccc ggctttaccg cgctgccttt ttttacgccc tccaaaccac
     6841 cttcgtgtga atcaccccac ccccgccatc gttgcccaga gtgccgtgcg gccgctgccg
     6901 cgctgggcgc tgctgctgct gtgcctggcc tatgccgtgc cgggctttgt cgggcgggcg
     6961 ccctggcgca gcgacgacat ggcctcgttc ggctacatgc gcgaactggc gctgggtcac
     7021 accagttggc tgcacccgct gctggccggc ctgccgggtg acggcgacgg cttgctgcat
     7081 tactggctgg gggcctgggc catacaggcc ttgggtggct ggctgcccct ggatctggcg
     7141 gcgcgcgtgc ctttcatcgc tctgctgctg ctgaccctgt cggccacctg gtatggcgtg
     7201 tactacctgg cacgcggccc aggagcccag cccgtggcct ttgcctttgg cggcgaggcc
     7261 cagcctgccg actacgcccg tgccatggcc gatggcggcc tgctggccct gatcgcctgc
     7321 ctgggcctgg cccagctgtc gcatgaaacc acgggctacc tgacgcagct ggccagcacg
     7381 gccttgctgt tctatgccgg cgccgccctg ccctggcgca tggcctggcc gctgctggcg
     7441 ggcctgatcg gactgcttgg gctggtgctc agcggcgcgc ccagcatcgc cgtgctgctg
     7501 ggcctgggct gcgcctggct ggtgtggcat agccacggcc ccacgctgcg cacgcgcgca
     7561 accgcgctcg cgctcatgct ggcggtgacc ctgctggcag ccctgctggc ccagcaactg
     7621 gacctgtggc gctggcgcat tgtgagggtg gatgggccct gggaatggca gagcctggtg
     7681 cgactcttga cctggttcag ctggccggcc tggccactgg ccctgtggac gctgtggcgc
     7741 tggcgccagc tgatcgccag ccggcactgg caccgccacc tgtggttgcc gctgtggttc
     7801 gcgctggtga ccctgggcgc cacgctcgcc acgcgccccg ccaaccgcgc cctgctgctc
     7861 agcctgccgg cgctggcagc gctggcggcc tttgccctgc ccacgctcaa gcgcagcatc
     7921 gcggcgctga tcgactggtt cacgctgctg ttcttcagca tctcggccat cgccatctgg
     7981 gtggtctggc tgtcggtgca gaccggctgg ccggccaagc ccgcggccaa cgtggccaag
     8041 ctggcgccgg gctatgtgtc cacgttttct gtgctgccgt ttgccgtggc gctggtggcc
     8101 acgctggcct ggtgcctgct ggtcgcctgg cgcacggcgc gcaaccgtcc cgccatctgg
     8161 aagagcctgg tgctgcccgc cggcggtgcc acgctgggct ggctgctgct catgacgctg
     8221 tggctgccga tactggacta cggccgcagc ctggcgccgc aggtggccgg cgtgcgcgcc
     8281 gtcatcaccg agcgccaggg ctgcgtggcc gcctatggcc tgagccgcgc ccaggtggct
     8341 gcgctgatgt accacggcga attgcccatg gtcgatctgc cgcgggtggc gcagtgcgac
     8401 tggctggtgg ccgacccgga cgccagtcct tccgtggtca cgctgctgcc ccctgggcaa
     8461 tgggtcgagg tggccatcgt cagccgtccg acggatcgca acgataggct gctggtgctg
     8521 catcgcaggg acgacgggcg ctgatgtccg agctgtccac catctcgcgc cacgccctga
     8581 ccgtgctggc cgggcaactg gcggtgatgg cctttggtgt caccgacacc atcgtcgccg
     8641 gccgctatgc gcaggaggcg ctggccgcgc tgtccatagg ttcggcggtg ttcatcagcg
     8701 tgtacgtcgg gctgatgggc gtgctgcagg cgctgatgcc cgtctgggcc gagcagcgcg
     8761 gcgccaatgc ccaggccgcc attggccgga gcgtgcgcca gtcgctctac ctgtgggccg
     8821 tggccacggc gctgggcatg gccgtgctgc tgtcgcccga gccgatactg cgctggaccg
     8881 aggtgccgcc cgagttgcgc ggcgcggtgc gcgcctatct ggccatcctg gccttcggcc
     8941 tgccgccggc gctgctgttt cgcatctaca gcacgctcaa ccaggccctg ggccacccgc
     9001 agctggtcac ctggctgcag gtgggctcgc tggcgctgaa actgccgctg tctatctggt
     9061 tcaccttcgg cggcgccggc ctggcgcccc agggcgtggc gggctgcgcc tgggccacgc
     9121 tggtggtcaa ctacggcctg atgctgctgg gctgggccat gctgcgccgc cacgccatgt
     9181 atgcgccact gcagctgtgg cgcgctctgg agcgccccga cggcacgcag atcgcgcgct
     9241 ttctgcgcct ggggctgcct gcgggtctgg ccatcctggt ggaggtgacc tcgttcacgc
     9301 tgatggccct gttcatcgcg cgccaggggg ccctggcctc ggccgcgcac cagatcgctg
     9361 ccaacctggc ggcggtggtc tacatggtgc cgctgtcgct ggccattgcc accagcgccc
     9421 gcgtgagcta ctggcgcggt gcgggcgacg agcgccaggc cggtgccaca gccttgaatg
     9481 gtttttggct cgcagcgctt atgggatgcg cgcttgcagc tacgcttgta atagcaaagc
     9541 aaccgattgc cgggctgtac tcgaccagcc cggaggtggt ggcattggcc gcatcgctgc
     9601 tggtgtgggt agccgcctat caggtggccg acgcgctgca gacgctgtgc atcttcgtgc
     9661 tgcgcagcta tcacatcaca ctcgcgcccc tggtggtgta tggggtgctg ctgtggggtg
     9721 tggggctgca tggcggttac cgcctggcct accagggctg ggctggctgg gggccgtatc
     9781 actccccagc ccccttctgg gccgccagcg ccgcggccct ggcggtaacg gccctggtct
     9841 ttgcggcgct gctgctgggc gtgctcaggc tgtcacggcg caaggcagac gagcgccagg
     9901 tgcacgcctg aagcccaggc caggcctgca ccagacaaag ccactgcgct caggcggccc
     9961 gtgcggcgtc cgccaggcgc tgggcgcagc ggctggcctg gtctgcgtcg cgtgcctcga
    10021 ccatcacgcg cagcagcggc tcggtgccgc tggcgcgcac cagcaggcgg ccgttgtcgg
    10081 ccagctcggc ctgtacctca cgcgtggtgt cggcgagcgc ggcgttcgcc ttccagtcct
    10141 gatcgggttt gaggcgcacg ttgagcagca cctggggaaa cagcggcaca tcggcaagca
    10201 actcggccag cgtcttctgg ctgcgcacac aggcctgcag cacctgcagc gcgctgatca
    10261 ggccatcgcc cgtggtgtgg caatcaagcg ccagcagatg gcccgagccc tcgccaccga
    10321 gcagccagtt ctgacgctgc agctcttcaa gcacatagcg gtcgcccacc ttggcgcgca
    10381 cgaacttcac accgcgcctt gtcagcgcct gctccacggc catgttggtc atcagggtgc
    10441 cgaccacgcc gggcacatgc tcgtcacgtg ccaggcgctc ggcggccatc aggtagagca
    10501 gctcgtcgcc gttgtacagg cggccgctgg catcgacgat ctgcaggcgg tcggcatcgc
    10561 catccagggc cacgccaaag tcggcctggt tggcgcacac ggcccgtacc agcgcttcgg
    10621 ggtgggtggc acccacctca tggttgatgt tcacgccatc gggggcacag cctatggcga
    10681 cgacgtcggc gcccagttcg tggaacacca tgggggcgat ttggtaggcg gcgccatggg
    10741 cggcatcgac gacgatcttc acgccgcgca gactcaggtc gtggtcaaag gtgctcttgc
    10801 agaactcgat gtagcggccg gccgcatcat cgagccggcg cgtcttgccc aaggatgccg
    10861 aatcggccca catgggcggc tgctgcagcg ctgcctcgac ctcttcctcc caggcgtcgg
    10921 gcagcttggt gccctgggcg ctgaagaact tgatgccgtt gtccgggtag gggttgtggc
    10981 tggcgctgat caccacgccc aggctggcgc gctgcgcgcg cgtgaggtag gccacgcccg
    11041 gcgtgggcag cggcccgagc agcaccacat cgaccccggc ggagttgaaa cccgattcca
    11101 gcgccgcctc cagcatgtag ccggaaatgc gcgtgtcctt gccgatcagc accgtggggc
    11161 gctcctcggt gcggcgcagc acgcgcccca ccgcatgcgc caggcgcaac acgaagtcgg
    11221 gcgtgatggg cgcctggccc acggtgccgc ggatgccatc ggtgccaaag tattgacggg
    11281 tcatggggtg cgtcctctct tgttcaatgg gtctgttcta taggggtctg ctcttgcatg
    11341 gcgcgccatg tggccagcgc gccgacggtg tcgcgaacat cgtgcacgcg cacgatgcgc
    11401 gcgccctgct gcacggccag caaggcggcc gccacgctgg cgcccaggcg ctcctgcacc
    11461 ggcaggccgg tgaccgcgcc cagcgacgat ttgcgtgacc agccggcaag cacggggtag
    11521 cccaaggcca gcagctcgcg ctggcgcgcc agcagtgcaa agttctgctg tacggtcttg
    11581 ccaaagccta tgccggggtc gatggcgatc cgagatttat tgacacccaa gccttgtaga
    11641 tattgcgcct gcagctctaa aaaagtgagt acctgcggca cgacatctcc ctgcatgggg
    11701 gccgtctgca tggtctgcgg gtcgcggtgc atgtgcatca ggcacaggcc gcagcgcggg
    11761 tgcgccgcca ccacctgcgc cgcgccgctt tggcgcaggg cccagatgtc gttcacgatg
    11821 tctgcgccca gatccagcgc ggcctgcatc acctggggct tgtaggtatc gaccgagatg
    11881 ggcacgccca ggcgcacggc ctcccgcatt accggcaaga cgcgttgcag ctcgtcctgc
    11941 aacggcagcg ccgggctgcc agggcgggtg gattcgccgc caatgtccag aatgtccgcc
    12001 ccctccttga gcaactgctc gcagtggcgc agggccgccg ccgtgccggc gtgctggcca
    12061 ccatcggaga acgaatcggg cgtgacgttg acgatgccca tgacccgcgc atgcgtcagg
    12121 tcgaggtcaa agcgggcggt ttgccaaagc atggggctgg gatagatgga caaacggggc
    12181 cccaaggccc cgtgaatcaa acgctgcggc ctctcatcag gcggcagtcg gcgtgggatc
    12241 gggcttcacg gcaggcgtgc cgccacccga gccatcgcca ccggaggggg gcgtgcgtgg
    12301 cgtccagtcc ttcgggggac gcggctcctt cccggccatg atgtcgtcga gttgctcggc
    12361 gtcgatggtt tcccactcca gcatggcctt ggccatggcg tgcatcttgt cctggttgtc
    12421 ctcgataagc ttgcgcgcca gggcatattg ctcgtcgatg atgcggcgca cctcggcgtc
    12481 caccttctgc atggtttctt ccgagatatt ggtggtcttg gtgaccgagc ggcccaggaa
    12541 gacctcgccc tcgttctcgg cgtagaccat ggggcccagg gccgccgtca tgccatagcg
    12601 catgaccatg tcgcgcgcga tcgaggtcgc gcgctcgaag tcgttgctgg caccggtggt
    12661 catctggtgc atgaacacct cttcggcgat acgtccaccg aacaacatgg cgatctggtt
    12721 cagcatgtac tccttgtcgt aggagtagcg gtctttctcg ggcaggctca tggtcacgcc
    12781 cagggcccgc ccgcgcggga tgatggtgac cttgtgcacc gggtcgcatt tgggcagcag
    12841 cttgccgatg agcgcatggc cggcctcgtg gtaagcggtg ttgcggcgct cctcctcggg
    12901 catgaccatg ctcttgcgct ccgggcccat gatgatcttg tccttggcct tctcgaagtc
    12961 ctgcatctcc acggtgcgcg cattgcggcg cgcggccatc agggcggcct cgttgcagag
    13021 gttggcaaga tcggccccgc tcatgccggg cgtgccgcgt gcgatgacgg ccgggttcac
    13081 gtcctggccc acggggatct tgcgcatgtg cacgttcagg atctgctcgc gaccacggat
    13141 gtcgggcagc gtcacataca cctggcggtc aaagcgcccc gggcgcagca gggccgcgtc
    13201 cagaatgtcg gggcggttgg tggcggcgac cacgatcacg cccaggttgg tctcgaagcc
    13261 atccatctcg accagcatct ggttgagcgt ctgctcgcgt tcgtcgttgc cgccgcccag
    13321 gcccgcgcca cgctggcggc ccacggcgtc gatttcgtcg atgaagatga tgcagggggc
    13381 gttcttcttg gcgttctcga acatgtcgcg cacgcgcgcc gcgcccacgc cgacgaacat
    13441 ctcgacgaag tcggagcccg agatggagaa gaacggcacc ttggcctcgc cggcaatcga
    13501 cttggccaac agcgtcttgc cggtgcccgg cgggcccacc agcagcaggc cacgcggaat
    13561 gcggccgccg agcttctgga acttctgcgg gtccttcaaa aagtccacga cttccttgac
    13621 ctcttccttg gcctcgtcgc aaccggccac gtcggcaaag gtgacggtgt tgttgttctc
    13681 gtcgagcatg cgcgccttgg acttgccgaa gctgaaggct ccacccttgc cgccgccctg
    13741 catctggcgc atgaagtaca cccacacgcc gatcagcagc agcatcgggc cccagctgac
    13801 cagcagcgtc atgagcagcg agccttcctc gcgcgggcgc acgtcgaact tgacgttgtt
    13861 gttgaccagg tcgccaatca ggccccgatc cagataggtg gccgtggtgc ggatgcgacg
    13921 atcatcattg gtgatggcga cgatctcggt gctgccgccc tggccttcct ggatggtggc
    13981 gctcttgatg cggtggttgc gcacgtcctc caggaactcc gaatagccca cagccccggc
    14041 gccaacgccc gtgcgggtgt caaactgttt gaacaccgta aagagcacca tggctatgac
    14101 cagccagact gcgacttttg aaaaccactg attgttcaag cggggctcca gattcagaaa
    14161 aagatgaacg gccaggaaac tgtcatgggg acgtcctggc gcatttgggg gtcattttag
    14221 gcgtttcaac cactgcccgg cgctggatta accccggcgc ggcccatggg caaagatcag
    14281 gcggttggct tgagccctat tccgaccaaa aaggtctcgg aagaccggtc gcgcgaggcc
    14341 ttgggcttga ctggcttgac cacgcggaag gtgtccttga acagctgcac cagctgcgcg
    14401 tagccactgc cgtggaacag tttcacgacc agggcgccat cgggcttcag gtgatgcacg
    14461 gcaaagtcca ccgccagttc gaccaaatgg gcaatgcgca cggcatcgac cgactccaca
    14521 cccgagagat tgggcgccat gtccgacacc accaagtcca cgggccggcc ctgcacggcc
    14581 tgctgcaggc gcgccagcac ctcgtcgtca cgaaaatcgc cctgcaggaa caccacgccc
    14641 tcgatgggct ccatgggcag gatgtccagc gcaatgatgg tacccgccag ctgccccacg
    14701 gccgcgccac ccggcgacag gcgccggcgc acatactggc tccatgcgcc tggcgcgctg
    14761 cccagatcca ccaccgtatg gccgggctgg atcagcccga actgctcatc gatttccttg
    14821 agcttgtagg ccgcgcgggc gcgatagccc tctttttgtg cgagcttgac gtaagggtcg
    14881 tggacatggt cattgagcca cgccttgttg accttcttgc tttttgtctg gactttcatg
    14941 ccttgagata atacggccta tgcctcaaat tcaactgacc ccggccgagc gccgggaaca
    15001 ccgcgccaat gcccaccacc tcgatcccgt ggtcatgatc ggcggcgatg gcctgacccc
    15061 ggcggtgcaa aaggaaatcg acgccgccct gaacgcccac gggctgatca aggtgcgcgt
    15121 attcggtgac gaccgcgccg cgcgcgaggc catgtaccag cagctggcgg gagagctgaa
    15181 cgccgccccc atccagcaca ttggcaagct gctggtgctg tggcgcccca tcccggccaa
    15241 ggacaagccc ctggacgaag accgcaagag cggcccgcgc gacgtcaagg ttttgaagta
    15301 cggccggccc ggccaacgcc ccgaggtcaa acagctgcgc gtgctgggta accagcgcct
    15361 gacatccggc ggccagatca agcgcgccaa gcccaagctg aaatccgtca agaaaaacca
    15421 gggccagtga tgacctcgcc gcgccagcgc cacatcctgt gcatgaaatg gggcaccaag
    15481 tacggccccg agtacgtcaa ccggctctac gccatggtgc gccgccacct gagcggcgac
    15541 tttaacttcg tctgcctgac cgacgacagc gccgggatca ggcccgaggt gcaatgcctg
    15601 ccgattcccc ccctgaacct gcccgccggc atacccgagc gcggctggaa caagctggcg
    15661 acgtttaccg gtgatctgta cggcctgaaa gggacggcgc tgttcctgga cgtggatgtg
    15721 gtcatcaccg gcagcctgga tgatttcttc acccagccgg gcgactttct catcatccac
    15781 gactacaagc gcccctggcg catcaccggc aactcgtcgg tgtaccgctt cgagctgggt
    15841 gcgcacccgg atgtgctggc gtactttcgc ggccatttcg aagaaatcag gcgccagtac
    15901 cgcaacgaac aggcctacct gtcggacttt ttgcacaagc agggcaagct gcagtactgg
    15961 cccaaggact ggtgcccgag cttcaagtac cacggcattc cgccctggcc gaccaactac
    16021 tggaaggcac ccttcgtgcc cgaaggtgcg cgcatcgtca tcttccacgg cgaatgcaac
    16081 ccgcccgacg ccctggccgg ccggcgcaac cggcgctttc gccatatcca gcccgccacc
    16141 tgggtggccg aacactggcg cgaataaacg cgctacctgc gcgccgtgcg ccagagtacg
    16201 cacagcgcac acagccactg cagggcatac atggcactgc ccgcaccgtg ccacaggcgc
    16261 aggttctgcc gcgccaggat gcgcggggcc acgccgtact gctgcagcag cgccagcagc
    16321 aggccggcaa tcacaaaaac catagctgct tgcgcccatg gctcctgctt ttcagcatgt
    16381 ttatgctttg aaattaccaa caataaagcg caacacgcta tagaaatata ggtctgtgcc
    16441 tcgaacaggc gcgccgccat gttccccgcc agcgctggcg tgggcaggtg ggcgaacagc
    16501 agcggcacgg cgacaaaacc catggcgcac aggctgcccc accacagggc ggcggccaac
    16561 accggcaggc gttcacgcat ggccggcaga ccgtcagagg tagctgacgg tgaccacttc
    16621 gtagcggcgc agcccgcccg gagcctgtac ctcggcggtg tcaccctctt ccttgccgat
    16681 cagcgcgcgg gcgatggggc tggacacatt gatcaggcca tgcttcaagt cggcctcgtc
    16741 ctcgcccacg atctggtact tgacggtgtc gccgctgtcc tcgtcttcga gctcgacagt
    16801 ggcgccaaac accacgcggc cgccggcatc gagttgcgag ggatcgatga tctgcgcggc
    16861 cgacagcttg ccctcgacct ccatgatgcg gccctcgatg aagccctggc gatccttggc
    16921 ggcctcgtac tcggcgttct cgctcaggtc gccctgagcg cgcgcctcgg caatggcgtt
    16981 gatgaccgcg gggcgttcca cggtcttgag gcggtgcagc tcggccttga gcttttcggc
    17041 gccgcgcttg gtgatgggaa tggtggccat gatcgaagtc tccagattcc aaaaaagcaa
    17101 aaagcaaacc gccgcgcgtt gccgcgcggc ggtcttctca agggcgaatt atgccgcgtt
    17161 tgcctgcgcg ctcaagcggt gggcagctgc gcatgcagct cctgcaccga atacacgtcg
    17221 agcttgtcca ggtgcttcat gccctccacc gccgcctcgg cgccaaagat ggtggtgaac
    17281 gtggtcacgc gcgactgcag cgagctggtg cggatggcac gcgaatcggc aatggcattt
    17341 cggcgctctt ccacggtgtt gatgaccatg gcgatctcgt cgttcttgat catgtccacg
    17401 atgtgcggcc ggccctcggt gaccttgttg accacctgca cggccacgcc ggcctcggca
    17461 atcgccgccg ccgtgccctt ggtggccagc agctcgaagc ccatggcgga aagatcgcgc
    17521 gctatggcca cggcacggcc cttgtcggca ttcttcaccg tgaggaacac cttgcccccg
    17581 gttggcaggc gggtagaggc gccgagctgg ctcttgacga aggcctcgcc aaacgtcttg
    17641 cccacgccca tgacctcacc ggtggacttc atctcggggc cgaggatggt gtccacgccg
    17701 gggaacttga cgaaggggaa cacggcctcc ttcacgctga agtacggcgg cgtcacctcg
    17761 cgggtcacgc cttggctggc gaggctctgc cccgccatgc agcgcgccgc caccttggcc
    17821 agctgtatgc ccgtggcctt tgatacaaag ggcacggtgc ggctggcgcg cgggttcact
    17881 tccaggacgt agatcacgtc gccggcgtcg gtttcttgaa tggcgaactg cacgttcatc
    17941 aggcccacca cgttcaggcc atgggccatg gccgtggtct ggcgcttgat ctcggccacg
    18001 gtgtcggcct tgaggtagta cgggggcagc gagcaggcgc tgtcgcccga gtgcacgccc
    18061 gcctgctcga tatgctccat cacgccgccg atgaagacgg cgccggtggc gtcgcgcacg
    18121 cagtccacgt cgcactcgat ggcgttggac aggaagcgat ccagcagcac gggcgaatcg
    18181 ttgctgacct tgacggcctc gcgcatgtag cgctccaggt cgcgctgctc gtgcacgatc
    18241 tccatggcgc ggccgcccag cacgtagctg gggcgcacca ccagcgggta gcccagggcg
    18301 gcggcctttt ccagcgcctc ggcctcggtg cgggccgtgg cgttgggtgg ctggcgcagg
    18361 cccaggtcgt tcaagagctt ctggaagcgc tcgcgatcct cggcggcatc aatcatgtcg
    18421 ggtgtggtgc cgatgatggg cacgccctca cgctccaggc ccagggccag cttcaaaggc
    18481 gtctggccac cgtactgcac gatcacgccc tcgggctttt ccttgtccac gatctccagc
    18541 acgtcctcca gcgtcagcgg ctcgaagtac aggcgatccg aggtgtcgta gtcggtggac
    18601 acggtctcgg gattgcagtt gaccatgatg gtctcgtaac cgtcctcgcg catggccagc
    18661 gccgcgtgca cgcagcagta gtcgaactct atgccctggc cgatgcggtt cgggccaccg
    18721 cccagcacca tgattttctt cttggttgtc ggcgctgcct cgcattcctc gtcgtagctg
    18781 gaatacatgt acgcagtatt cgtcgggaat tcggccgcgc aggtatcgac gcgcttgtag
    18841 accgggcgca cgttcagggc acggcgtgct tcgcgcaccg ccttctcagt ggtatgcagc
    18901 agcttggcca ggcggcggtc ggaaaagccc ttctgcttca aggcgcgcag ggtggcggcg
    18961 tccagcaggg ccagcgcctg gtcacccttg tcggcataga gtttgtccag gtcgagttca
    19021 atcttcacga tctcctcgat ctgcaccagg aaccaacgat cgatcttggt gagatcaaac
    19081 acctcatcga cgctcatgcc ctgggcgaag gcgtcgccca cataccaaat tcgctcgggg
    19141 ccgggctcgc ccagctcctt ttccaggatc tcgcgatcct gggtcttctc gttcatgccg
    19201 tccacgccca cttccaggcc gcgcaacgcc ttctggaagg actcctggaa ggtgcggccc
    19261 atggccatga cctcacccac actcttcatc tgcgtggtca ggcggctgtc ggccgtgggg
    19321 aacttctcga acgcaaagcg cgggatcttg gtgaccacat agtcgatcga gggctcgaac
    19381 gaggccggtg tggcgccgcc ggtgatctcg ttcttcagct cgtccagcgt atagcccacg
    19441 gccagcttgg cggccacctt ggcgatcggg aaacccgtgg ccttggaggc cagcgcgctc
    19501 gagcgcgaga cgcgcggatt catctcgatc acgatcatgc gcccgtcctt ggggttgacc
    19561 gcaaactgca cgttggaacc acccgtgtcc acgccgatct cgcgcagcac ggcgatgctg
    19621 gcgtcgcgca ggatctggta ctcacgatcg gtgagggtct gcgccggggc cacggtgatg
    19681 gaatcacccg tatgcacgcc catggggtcg aggttctcga tcgagcagat gatgatgcag
    19741 ttgtccgcac ggtcgcgcac cacctccatc tcgtactctt tccagcccag cagcgactct
    19801 tcgatgagca gctcgttggt cggcgaggcc tccaggccgc gcttgcaaat ggtctcgaac
    19861 tcttccgggt tgtaggcaat gccgccgccc gtgccgccca gcgtgaagct gggacggatc
    19921 accgtgggaa aacccacatt cttctgcacg gcccaggctt cgtccatgct gtgtgcaatg
    19981 cccgagcgtg ccgacgccag gccgatgcgc gtcatcgcgt ccttgaactt caggcgatcc
    20041 tcggccttgt cgatggcctc gggcttggcg cctatcagct cgacacggta tttgttcagc
    20101 acgccgttgc gccacaaatc cagcgcgcaa ttgagcgcgg tctgcccgcc catggtgggc
    20161 aggatggcgt ccggacgctc cttggcaatg atcttttcca ccgtctgcca ggtgatgggc
    20221 tcgatgtagg tcacatcggc cgtggccggg tcggtcatga tcgtggcggg gttgctgttg
    20281 atcaggatga cgcggtagcc ctcctcgcgc agcgccttgc aggcctgcac gccggagtag
    20341 tcgaactcgc aggcctggcc aatgatgatg gggccggcgc cgatgatgag aatgctttga
    20401 atgtctgtgc gcttgggcat tatttcttct cctggcctgc tgctgccatg gacgccatga
    20461 aacggtcgaa caggtggcca atgtcgttgg ggccgggcgc ggcctccggg tgaccctgga
    20521 agctgaaggc aggcttgtcg gtgcactcca ggccctgcag cgtgccgtca aacaggctca
    20581 catgggtggc gcgtgcgctg gacggcagcg tggccgcatc cacggcaaag ccgtggttct
    20641 ggctggtaat gcagacatgg ccgctggcca gcgtcttcac cggatgattg ccgccatggt
    20701 ggccatgggg catcttgaag gtctgggcgc ccagggccag ggccatgatc tggtggccca
    20761 ggcagatgcc gaaggtgggg atgccttggt ccaggaaggt gcgtgccgcc tcgatggcgt
    20821 agctgcaggg ctgcgggtca cccgggccgt tggacaggaa gatgccatcg ggctgaaggg
    20881 ccagcgcttc ctcggccgag gtctgcgccg gcaccacggt cagcttgcag ccacgctcgg
    20941 ccagcatgcg caggatgttg cgcttgacgc caaagtcata ggccacgaca tgaaaacgcg
    21001 gcttgtccag cttgccaaag ccctggccca gctcccattc ggtctgctcc caggcatagg
    21061 gctgctgcgt gctgaccacc cgggccagat ccaggcccgc catgctgggg gcgttcttcg
    21121 cggcggcgat ggccgcggct atgcgctcgg gcgtcaccgc ctcgctggcg gccagaccca
    21181 cgatggcacc gttttgcgta cccttttcac gcagcaggcg cgtgagcttg cgggtatcga
    21241 tattggcaat cgccacagca ccctgcgcgt tcaggaactg cgacagattt tgctgcgcac
    21301 ggaaattgct ggcgatgagc ggcaggtcgc gaatgaccag gcccgcagcc tgcaccttgg
    21361 acgattccat atcctcctca ttgacaccat agttgccaat gtgcggatag gtcagggtga
    21421 cgatttgctg gcagtagctc gggtctgtga gaatttcctg gtagccggtc atggcggtgt
    21481 tgaacaccac ttcgccgaca gtcgtgccga cgctaccaat ggaattgccg ataaacaccg
    21541 tgccgtctgc gagcgccaga atggctggcg ggaaagctgc cttgagtgac gaaagcactg
    21601 ggatctccga aatgagtcgg gtcgcccgga gcccacaaaa ccttgtttgc ggactgctgc
    21661 ttgttgggga gttgctttgg atgcgtccgg gcgacgtttt tgcagaaaag cctctcatta
    21721 taaagggagg acacgccatc aagcccgaga ggtttttctc gcaacaggcc cgaaaatagg
    21781 cacccgcgcc gaaccaaaaa ccaaaagggc caccgcgtaa acggtggccc ttttcaatca
    21841 atggtcggcg tggcgggatt cgaactcgc
//
LOCUS       DAOMTQ010000034        21082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_293_length_21082_cov_25.887098, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000034 DAOMTQ010000000
VERSION     DAOMTQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 21082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21082
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_293_length_21082_cov_25.887098"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK518_05245"
     CDS             <1..673
                     /locus_tag="PK518_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRM48351.1"
                     /translation="MRAGGRGARIHWARTLRQAARHGGDLVALQRQQRRQQALPLLVL
                     VDVSGSMERYARLLLAFIHAATARVRVDGQAQRLRREVFAFGTGLTDLGPAFRESDTD
                     AMLQRAGQAIHDFAGGTRLGDSLARLRQQHARRLVGRRTLVLIVSDGLDTGEPEDLAR
                     ELDWLKHRCRRLLWLNPLLRFDGYEPSARGAAVLHRAADAMLAVHNVTRLQQLAGAIA
                     SLMKQ"
     gene            691..1242
                     /locus_tag="PK518_05250"
     CDS             691..1242
                     /locus_tag="PK518_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HRM48352.1"
                     /translation="MEMQGARQLAITQQQAWEALNDPEVLKLCIPGCDSIEATGDNAY
                     ALVNAIKVGPVAAKFKGAIALADVNAPHSYTLNFEGNGGVAGFGKGSAQVSLAPQEEG
                     CELAYSVNATVGGKIAQVGQRLIDGVAKSMAESFFKRFDEEMQRRHGPPPEETEEKPG
                     ALKKMWSRLKGGDKDAASPAEET"
     gene            1245..2285
                     /locus_tag="PK518_05255"
     CDS             1245..2285
                     /locus_tag="PK518_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRM48353.1"
                     /translation="MENLDVTVLRALRDWRLAGKPALLVTVVRTWGSSPRPVGSIMAL
                     CADGSVMGSVSGGCIEDDLIARYAHAAGADALPRSGPPQFAKYGVTADEAFRFGLPCG
                     GTLELLLEFDPDGASIAALVQALDAGRLMHRRVRLGDGAVTLEATDAPAELVLGGAQL
                     TNTFGPEYRMLIIGAGQMSEYLATMAQFCGFAVSVCDPREEYRGAWSVPGVRLLTSMP
                     DDTVAAFAPDARSCVVALTHDPKLDDLALLQALETPAFYIGAIGSRRNNAARRQRMIE
                     HLGQAEESLARLRGPIGIYIGSKTPPEIAVSVMAEVLAVKNGVPLPRDMDVAHVKDSQ
                     GQAAAGSACKVG"
     gene            2285..2956
                     /locus_tag="PK518_05260"
     CDS             2285..2956
                     /locus_tag="PK518_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM48354.1"
                     /translation="MSPMDAVAAVVMAAGAGRRMGYFPKSLLERDGEPLLLRQIRLLG
                     EAGASTVVVVLGHHAARLRAVLAQAAPRLPITLRWVLNPAPDEGPGGSLRCGLAALPP
                     DVDTLLVLLGDQPLLEPQDLQALLAAWRARPAGVELVLPQHAGQPGHPIVFGPLLRRA
                     VMQAQGGRGVREWRRAHPAQVQALALEHARCTTDVDTPEDLRRLGERWGVWIRPPRPA
                     DPAAP"
     gene            complement(2919..3797)
                     /locus_tag="PK518_05265"
     CDS             complement(2919..3797)
                     /locus_tag="PK518_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication/partition related protein"
                     /protein_id="HRM48355.1"
                     /translation="MNPETIVVLPELKAYIDPLTPDEHDALERSILAEGCRDALVLWG
                     DVLVDGHNRYGICQQHGLPFQTVQNTRFQSMEDVHLWMIDQHLGRRSVSEFQRGVLAL
                     KKREIIAERRAQAAAAVVAAKAEAAQSPGGQAPWEGDTDPVVAKALATVAKVPEDALD
                     TREALARAARLTAAQVKAIEAIHQNAAPEVVAAVKSGELSLNAAAVVATLSVEEQQAA
                     AAGGAQDLKQAAKRVREAKKKPKAGTQDMAAEGEAAAAPPDAAQLRQRVTELEAENER
                     LRQQVKALQDLLAEAA"
     gene            complement(3988..5358)
                     /gene="yegQ"
                     /locus_tag="PK518_05270"
     CDS             complement(3988..5358)
                     /gene="yegQ"
                     /locus_tag="PK518_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HRM48356.1"
                     /translation="MTLKAPELLLPAGSLDKMRAAYDFGADAVYAGQPRYSLRARNNE
                     FRLEQIAQGIQEAHARGKKFFVTSNLIAHNDKVRTYLRDIEPVIACQPDAFIMADPGL
                     IMMVKEKWPETEIHLSVQANTTNWATVKFWQKMGVARIILSRELALDEIEKIRQECPD
                     MEIEVFVHGALCIAYSGRCLLSGYFNHRDPNQGTCTNACRWEYKTHDAQVDPNTGEAL
                     ASTMDKGFSFTKSREDADAQFTGTAIQRHPKADQVYLLEEANRPGELMPIMEDEHGTY
                     IMNSRDLRAVEHVQRLVQIGVDSLKIEGRTKSLYYVARTAQVYRRAIDDAVAGRPFNP
                     ELITELEGLANRGYTGGLLERRPANDYQNYETGHSVLQRSHFVGEVRGYADGLAEVET
                     KNRFAVGDRLEIIHPQGNRQVLLEKMFNLDGEPVQVAQGSPVRVRIPLAGPVEGALIA
                     RLLQPS"
     gene            complement(5501..6490)
                     /gene="tolA"
                     /locus_tag="PK518_05275"
     CDS             complement(5501..6490)
                     /gene="tolA"
                     /locus_tag="PK518_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HRM48357.1"
                     /translation="MQAAIHDRDQFAPPRPPGRMRAITLAVLAHAVLVVALTWGVNWK
                     SSSDQPAVEAELWAAVPQQAAPRAETPPPPPAPEPTPEPQPAPAPPPPPPPAPRQAEP
                     DTRDADIALQKQKKREEELKKKREQELEQDKKDKERRERLEQEKKERQLAQKKAEREK
                     AEREKEQREKQLAEQKKLEQQKAEQEKQKKLAEDKRRKAEEAREAREADARHQANIRR
                     MQGLAGATGGETATGTALRSAGPSGGYGGKVAAKVKPNIVFPDQVDGNPRAEVEVLAA
                     PDGTIVKARITQSSGNKAWDEAVLRALQRTETLPRDIDGRVPSSLVISFRPKD"
     gene            complement(6513..6938)
                     /locus_tag="PK518_05280"
     CDS             complement(6513..6938)
                     /locus_tag="PK518_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRM48358.1"
                     /translation="MPAMASRSGSRRRSMNEINMVPFIDVMLVLLIIFMVTAPMLTPS
                     SVNLPSVGKGAKLPKTRADVIVDKDGGVRFKADGNERTVPLAQLGHTAKTWLKDQPED
                     TPVLISADKTASYDSVMKAMSELQRAGVARVALAVKSGG"
     gene            complement(6952..7653)
                     /gene="tolQ"
                     /locus_tag="PK518_05285"
     CDS             complement(6952..7653)
                     /gene="tolQ"
                     /locus_tag="PK518_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRM48359.1"
                     /translation="MNSQDMSMLSLVLGASWVVQLVMLLLLAVSVASWAAIFRKLFAL
                     KRVKALNEDFERDFWSGTSLNELFAAAAHNAKSAGPMERIFASGMREYQKLRERRISD
                     AGTLLDGARRAMRASFQREMDVIESSLSFLGSVASVSPYVGLFGTVWGIMHAFTGFAG
                     MEQVTLATVAPGIAEALVATAIGLFAAIPAVIAYNRFARDIDRVATHQETFIEEFSNI
                     LQRNLGAQPGSPSGH"
     gene            complement(7650..8069)
                     /gene="ybgC"
                     /locus_tag="PK518_05290"
     CDS             complement(7650..8069)
                     /gene="ybgC"
                     /locus_tag="PK518_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HRM48360.1"
                     /translation="MAFEWPVRIYWEDTDAGGIVFYANYLKFFERARTEWLRALDLGQ
                     QQLREQTGGMFVVTDARLRYLRPARLDDELIVTARLQDSGRASMTIEQQAFLTTQATP
                     AQPQLLCEASIRIGWVDGTSMRAARIPDSVLQAISRS"
     gene            complement(8076..9260)
                     /locus_tag="PK518_05295"
     CDS             complement(8076..9260)
                     /locus_tag="PK518_05295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRM48361.1"
                     /translation="MKFSTRAERIEPFYVMEVAKAAQAMAREVAGTHEPMIFLNIGEP
                     DFTAPPLVQQAAERAIRDGLTQYTNALGLEPLRERISDWYATRFGVDVPARRIVVTAG
                     ASAALQLACLALIDAGDEVLMPDPSYPCNRHFVSAAEGKAVLIPTTAEERFQLSAAKV
                     EAAWGEKTRGVLLASPSNPTGTSIAPDELRAIHQVVRAKGGLTIIDEIYLGLSYEEQY
                     SHSALALGDDIISINSFSKYFNMTGWRLGWMVVPDAMVPVVERLAQNLFICASTISQH
                     AALACFEAESIAEYERRRAEFKARRDYFIPELNKLGLSVPVMPDGAFYAWADCTQVAH
                     RLGVDGSWDFAFELMRRAHLAITPGRDFGTADTARHVRFSTANSMAQLQEAVSRLQRL
                     IG"
     gene            complement(9316..9843)
                     /gene="nusB"
                     /locus_tag="PK518_05300"
     CDS             complement(9316..9843)
                     /gene="nusB"
                     /locus_tag="PK518_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRM48362.1"
                     /translation="MSEAPASKRPPRQSRTGTTSTGARKAGSKSARSRAREFALQALY
                     QHLVGGNDASSIDLFTRDLSGFHKADAAHYDALLHGCITTAQFMDELITPQLDRKLAE
                     ISPIEHAVMWIGVYEFQHCPDVPWRVVINECIELAKEFGGTDGHKYVNGVLNGLAPQL
                     RAAEVEADKAAGKAS"
     gene            complement(9840..10304)
                     /gene="ribH"
                     /locus_tag="PK518_05305"
     CDS             complement(9840..10304)
                     /gene="ribH"
                     /locus_tag="PK518_05305"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRM48363.1"
                     /translation="MFDADKGTAAQLNGSELHIGLVQARFNEGITNTLAAACRAELIK
                     LGVQEAHIHHVLVPGALEVPLALQAMAERDEYDALIALGCIIRGETYHFELVANESGA
                     GVTRVGLDYQIPIANAILTTENLEQAIARQTDKGVDAARVAVEMARLLEELQ"
     gene            complement(10308..11429)
                     /gene="ribBA"
                     /locus_tag="PK518_05310"
     CDS             complement(10308..11429)
                     /gene="ribBA"
                     /locus_tag="PK518_05310"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRM48364.1"
                     /translation="MNTPLTPAPISPVQDIVAEMRAGRIVILVDEEDRENEGDLVIAA
                     DHVTPEAINFMARFGRGLICLTLSRERCERLQLPPMVARNGTKMGTAFTVSIEAAEGV
                     TTGISAADRARTVQAAVAPNAQAADLVQPGHIFPLQAVDGGVLMRAGHTEAGCDLAAM
                     AGCSPSSVICEVMKDDGTMARLPDLQLFAAEHGLKIGTIADLIEYRSRTESLVHKVGS
                     RPMQTAFGEFTAHAFRDEPSQAVHLALVMGQWDAADSVPVRVHEPLSVLDTLEPSRAM
                     HSWSLDASLRHIAAQGRGVAVLLNCGETGEQLLEQFEGKARAAQAPERGRMDLRTYGV
                     GAQILREVGVARMQLMGQPRRMPSMTGYGLEITGYIPKE"
     gene            complement(11571..11864)
                     /locus_tag="PK518_05315"
     CDS             complement(11571..11864)
                     /locus_tag="PK518_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HRM48365.1"
                     /translation="MENTTPPFFIARVGDDGTQCDAWPEQPLLLSLEQGGIDWPSSCR
                     NGSCRTCIGMLADGEVRYAIDWPGLSPEEKAEGCVLPCVAYPMSDIRLEASAI"
     gene            complement(12182..12553)
                     /locus_tag="PK518_05320"
     CDS             complement(12182..12553)
                     /locus_tag="PK518_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRM48366.1"
                     /translation="MNGQDLPLWLDISVSLLVLAGAAIAFLGSLGLLRLKSYFERVHA
                     PAIIATMACWCVMHATLLYFSMVGEGLAVHALLIALFVAVTVPVTNIFLMRAALFRAR
                     RMGEAVPANLSRTATEDAREP"
     gene            complement(12550..12831)
                     /locus_tag="PK518_05325"
     CDS             complement(12550..12831)
                     /locus_tag="PK518_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HRM48367.1"
                     /translation="MTGSLLAWALPSALFMLVAAMVLCLVRLLKGPAAQDRVLALDCI
                     YLNGMLLMLVLGIYYGSQHYFEAAMLIALVGFASSTAMAKFLLRGEVIE"
     gene            complement(12828..13319)
                     /locus_tag="PK518_05330"
     CDS             complement(12828..13319)
                     /locus_tag="PK518_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRM48368.1"
                     /translation="MMKKILPAPLVSFGLFVVWLLLNRSLSPGHLLLALLLALGLPVL
                     FKELRPQKVRVRHLGTVLRLCWTVVVDTTQSNIAVLRFLLLPRTRRHSADFVKIPLEL
                     RDPNGLAVLAMIVCITPGTVWAELSRDRSMLMLHVLEVHDREAIVQHVQTRYERPLME
                     IFE"
     gene            complement(13316..15034)
                     /locus_tag="PK518_05335"
     CDS             complement(13316..15034)
                     /locus_tag="PK518_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HRM48369.1"
                     /translation="MSRLSEVTSALMPHLMLAPIALPMFTAALLLLLREEQQRLKFGV
                     SLVSTLVGLLVSIALLQWVHRQGAEVGMGVYLPGNWPAPFGIVLVLDRLTALMLLLTS
                     LVALCSVLFAGARWQRAGVLYQALFQFQLMGLAGAFLTGDLFNLFVFFEIMLAASYGL
                     LLHGSGRARVQSGLHYIAINLAASSLFLVGAAMLYALTGTLNMADLARAVPQVLPADR
                     GLLHAAAAILATAFLIKAALWPLNFWLVPAYSAATAPVGALFALMTKVGLYALLRLWS
                     LLFGADAGASAQFGGLWLILGGMATMAFGAIGMMGSQRLTHLASHAAVLSSGTLLAAA
                     GFGQDRLTAGLLYYLPSSTLAVAALFLLADLTERWRNDGATLAPYENDDDAPFLSAEL
                     VPTAGLNLDDEEQVLVGRVIPAATAFMGLAYLMSTLVIAGLPPLSGFIGKFAMLGPLL
                     DPQGLGTAPGTSPGPAGWTLLALMIGTGLVALMALTRSGIRHFWAASERDRPQLHVLE
                     GLPIALLLAACVVLTVQAEGVMRFTTATARALYAPAGYVRAVLNAPPRTGPGDSARPA
                     GKEARP"
     gene            complement(15031..15396)
                     /locus_tag="PK518_05340"
     CDS             complement(15031..15396)
                     /locus_tag="PK518_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HRM48370.1"
                     /translation="MEIILAISIGVLTGSGVWLLLRPRTFQVIMGLSLLSYAVNLFIF
                     SMGRLGLALAKEPVLLPGVAQDLQHYADPLPQALVLTAIVIGFAMTALFLVVLLAARG
                     ISGTDHVDGSQARDAQEMP"
     gene            complement(15397..18354)
                     /locus_tag="PK518_05345"
     CDS             complement(15397..18354)
                     /locus_tag="PK518_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HRM48371.1"
                     /translation="MSLVLLVALPFLASLLAALLPANARNAESTLAGVIALLCALLTA
                     LSFPDIAGGGVLRQEIAWLPTLGMNLILRLDGFAWMFCMLVFGIGALVVLYARYYMSP
                     EDPVPRFFSFFLAFMGAMVGVVLSGNIIQLVFFWELTSLFSFLLIGYWHHREDARRGA
                     RMALTVTGTGGLCLLAGMLILGHIVGSYDLDQVLAAGDLVRAHPLYLATLVLVLLGAF
                     TKSAQFPFQFWLPNAMAAPTPVSSYLHSATMVKAGVFLLARLWPVLGDTEAWFWLVGG
                     TGLATLLIGGYAAMFQDDLKGLLAYSTISHLGLITLLLGLNSPLAAVAAVFHIMNHAT
                     FKASLFMAAGIIDHESGTRDINRLSGLRRMMPITATLATVASAAMAGVPLLNGFLSKE
                     MFFAETVFLDASPLVSTLLPVGATVAGMFSVAYSLRFIVDVFWGPPATDLPYAPHEPP
                     HWMRVPVELLVLACLVVGVLPAWSIGRFLDAAALPVVGGQLPAFSLALWHGLNTPLLM
                     SFVALIGGAALYQALRSLRRLGRIGGPPLMRFISGRRVFEALLAKCTQAARKGRRLLS
                     TRRLQWQMLWLLLFTLVAGALPLWLGSMRLGGRAALALSPAFVLVWLLGNVCAVAAAW
                     QAKYHRLAALALMGGAGLCTSITFLWFSAPDLALTQLAVEVVTTILILLGLRWLPKRD
                     ESLPAASASTALTRVRRLRDLLLALAAGGGMAWLSFAMLSRPFAQSTSTFFLERALTE
                     GGGTNVVNVMLVDFRGFDTFGEIVVLGIVALTVYALLRRFRPAGEAMDLPEQQRNLPA
                     DLQTDLLNPRHAQDTAVGYLMVPAVLVRLLLPFTALVACYLFLRGHDQPGGGFVAGLV
                     FSVGLVLQYMISGTEWVEAHLPVFPRRWIATGLLLALGTGLGAVAVGYPFLTSHMFHL
                     QLPLLGQVHLASAMFFDAGVFALVVGSTLMMLTAIAHQSVRSHRYHARLLEEQQLAMA
                     AVVSYDDDAREAR"
     gene            complement(18449..18760)
                     /locus_tag="PK518_05350"
     CDS             complement(18449..18760)
                     /locus_tag="PK518_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM48372.1"
                     /translation="MRLRHSLCLAALLHLGAALAGTDINQAREADLDGIQGLGPALTA
                     RILVERARQRFQDWPDLIARVKGIGPATAARLSDNGLTVNGAPYAAPQTAPPARTDSR
                     D"
     gene            complement(18769..19926)
                     /gene="lapB"
                     /locus_tag="PK518_05355"
     CDS             complement(18769..19926)
                     /gene="lapB"
                     /locus_tag="PK518_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HRM48373.1"
                     /translation="MEFDFTWLLLGLPLAFGLGWLASRLDLRQLREENRRAPKAYFKG
                     LNYLLNEQQDQAIDAFIEAVQNDPDTTELHFALGNLFRRRGEYNRAVRVHEHLLSRGD
                     LSRADRERAQHALALDFLKAGLLDRAEDALRRLEGTPFEAQARMALLAIYERSRDWQQ
                     ASDITQRMQGAGQGDFSTRQAHYLCEQALALSARGELPAAQTLLLQAASTAPQAARPR
                     IELARLQQRMGQADAALSTLQTLAQTTPAALGLAAPLLVEAALASGQAEQAGALLRQH
                     YEDMPTLDLLEAVVTLEAATQGSATAARAWYMRHLDKEPSLVAASKWLAGETLSQEQF
                     HPQIQRALEQATKPLTRYRCAACGFEARQHFWQCPGCQSWDSYPARRVEEL"
     gene            complement(19916..20230)
                     /locus_tag="PK518_05360"
     CDS             complement(19916..20230)
                     /locus_tag="PK518_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRM48374.1"
                     /translation="MKYLLWLLKAAIFFTLFAFALNNQQDATVHFFFGTQWTAPAVLI
                     VLSAFALGVVVGVLGMVPRWWRHRSAARQAQAEAPPPSAAPAPPAPAAEPVAPHLPID
                     GI"
     gene            complement(20366..20659)
                     /locus_tag="PK518_05365"
     CDS             complement(20366..20659)
                     /locus_tag="PK518_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HRM48375.1"
                     /translation="MTRSDLVEELAARFKQLTQRDAETAVKTILDAVGDALVRGHRIE
                     IRGFGSFSVSHRPPRLGRNPRSGEAVQIPEKRVPHFKPGKALRESVDQPPQAS"
     gene            complement(20750..>21082)
                     /locus_tag="PK518_05370"
     CDS             complement(20750..>21082)
                     /locus_tag="PK518_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRM48376.1"
                     /translation="QTVTGKVKTVDARGAEIDLGEDIVGYLRASEISRDRVEDARNVL
                     KEGDEVTAVVVNVDRKTRNIQLSIKAKDQADQQEAMAHLSAQSAKENAGTTSLGALLR
                     AKLDNSDK"
BASE COUNT         3430 a         6878 c         7416 g         3358 t
ORIGIN      
        1 catgcgcgcg ggcgggcgcg gcgcgcgcat ccactgggcg cgcacgctgc gccaggccgc
       61 gcgccatggt ggcgacctgg tggcgctgca gcgccaacag cggcgccagc aggccctgcc
      121 gctgttggtg ctggtggatg tgtccggctc catggaacgc tacgcgcgcc tgctgctggc
      181 cttcatccat gccgccacgg cgcgcgtgcg cgtggacggc caggcccagc gcctgcgccg
      241 tgaggtgttt gcctttggta ccggcctgac cgacctgggc ccggcctttc gcgagagcga
      301 caccgacgcc atgctgcagc gcgccggcca ggcgatacac gactttgccg ggggtacgcg
      361 cctgggtgac agcctggcca gactgcgcca gcagcatgcg cggcgcctgg tcgggcggcg
      421 cacgctggtg ctcatcgtca gcgacgggct cgacaccggc gagcccgagg acctggcgcg
      481 cgagctcgac tggctcaagc accgctgccg gcgcctgctg tggctcaacc cgctgctgcg
      541 ctttgacggc tatgagccga gcgcgcgcgg cgctgccgtg ctgcaccgcg cggccgacgc
      601 catgctggcc gtgcacaacg tgacccggct gcagcaactg gccggcgcca tcgccagcct
      661 gatgaaacaa taaacccaag gagacagacc atggaaatgc aaggcgcacg ccagctggcg
      721 atcacccagc aacaggcctg ggaggccttg aacgaccccg aggtgctgaa actctgcatc
      781 cccggctgcg acagcatcga ggccacgggc gacaacgcct acgccctggt caacgccatc
      841 aaggtcggcc cggtggcggc caagttcaag ggcgcgattg cgctggccga cgtgaacgca
      901 ccgcacagct acacgctgaa cttcgagggc aacggcggcg tcgccggctt tggcaagggc
      961 agcgcccagg tgtcgctggc gccacaggaa gagggctgtg agctggccta cagcgtcaac
     1021 gccaccgtgg gcggcaagat cgcccaggtc ggccagcgcc tgatcgacgg cgtggccaag
     1081 tccatggccg agagcttttt caagcgcttt gatgaggaaa tgcagcgccg ccatggcccg
     1141 ccgcccgagg agactgagga aaagcccggc gcactgaaaa aaatgtggtc caggctcaag
     1201 ggcggtgaca aggacgccgc gtccccggca gaggagacat agccatggaa aacctcgacg
     1261 tcaccgtgct gcgcgcgctg cgcgactggc gcttggcggg caagccggcg ctgctggtca
     1321 cggtggtgcg cacctgggga tcgtcgccgc ggccggtggg ctccatcatg gcgctgtgcg
     1381 cggacggttc ggtcatgggt tcggtctcgg gcggctgtat tgaagatgat ttgatagcac
     1441 gctacgccca tgcggcgggc gctgacgccc tgccgcgcag cgggccgccg cagttcgcaa
     1501 agtacggcgt gacggccgac gaggcctttc gctttggcct gccctgcggc ggcacgctgg
     1561 agctgctgct ggagttcgac cccgacggcg ccagcatcgc cgccctggtg caggcgctgg
     1621 atgccggccg gctgatgcac cgccgcgtgc gcctgggcga cggcgccgtg acgctggagg
     1681 caacggatgc gccggcggag ctggtgctgg gcggcgcgca gctcaccaac accttcggcc
     1741 cggaataccg catgctcatc atcggcgccg ggcagatgag cgaatacctg gccaccatgg
     1801 cgcagttctg cggctttgcc gtcagcgtct gcgacccacg cgaggaatac cgcggcgcct
     1861 ggagcgtgcc cggcgtgcgc ctgctgacca gcatgcccga cgacaccgtg gccgcctttg
     1921 ccccggatgc acgctcctgc gtggtggccc tgacgcatga ccccaagctg gacgacctgg
     1981 cgctgctcca ggcgctggag acgccggcct tctacatcgg cgccatcggc agccggcgca
     2041 acaacgccgc gcggcgccag cgcatgatcg agcacctggg ccaggccgag gaaagcctgg
     2101 cgcgcctgcg cggcccgatc ggcatctaca tcggcagcaa gaccccgccc gagatcgcgg
     2161 tcagcgtcat ggccgaggtg ctggccgtga agaacggcgt gcccctgccg cgcgacatgg
     2221 atgtggccca tgtcaaggac agccagggcc aggcggccgc cggctcggcc tgcaaggtgg
     2281 gctgatgagc ccgatggatg ccgtggccgc cgtggtcatg gccgctggcg cgggccggcg
     2341 catgggctat ttccccaagt ccctgctgga gcgcgacggc gagccgctgc tgctgcgcca
     2401 gatccgcctg ctgggcgagg cgggggccag tacggtggta gtggtgctgg gccaccatgc
     2461 cgcccggctg cgggccgtgt tggcgcaggc cgctccccgg ttgcccatta ccctgcgctg
     2521 ggtgctcaac cccgcgcccg acgagggccc gggcgggtcg ttgcgctgcg gcctggcggc
     2581 gttgccgccc gatgttgaca ccttgctggt gctgctgggc gaccaacccc tcttggagcc
     2641 gcaggatctg caggccctgc tggccgcctg gcgcgcgcgg ccggccggtg tggagctggt
     2701 gctgccccag catgccggcc agccgggcca ccccatcgtc tttggcccgc tgctgcggcg
     2761 cgcagtgatg caggcccagg gcgggcgggg cgtgcgtgaa tggcggcgcg cacatccggc
     2821 ccaggtgcag gcgctggcgc tggagcacgc gcgttgcacc accgacgtcg atacgcccga
     2881 ggatctgcgc cgactgggtg aacgctgggg ggtgtggatc aggccgcctc ggccagcaga
     2941 tcctgcagcg ccttgacctg ctggcgcagg cgttcgttct cggcctccag ctcggtcacg
     3001 cgctgccgca gctgcgcggc gtcgggcggc gccgctgccg cttcgccctc ggcggccatg
     3061 tcctgggtgc cggccttggg ttttttcttc gcctcgcgca cccgcttggc ggcctgcttg
     3121 agatcctgcg caccgcccgc ggcggcggcc tgctgctcct caaccgatag cgtggcgacg
     3181 accgcggcgg cgtttagcga caactcgcca gacttgacgg cggccaccac ctcgggcgcg
     3241 gcgttctggt ggatggcctc gatggccttc acctgggccg ccgtcaggcg cgcggcgcgg
     3301 gccagggcct cgcgcgtgtc cagcgcgtcc tcgggtacct tggcaactgt tgccagggcc
     3361 ttggccacca cggggtcggt gtcgccctcc cagggggcct ggccgccggg cgactgcgcg
     3421 gcctcggcct tggcggccac cacggcggcg gcggcctggg cgcggcgctc ggcaatgatc
     3481 tcgcgcttct tcagcgccag cacgccgcgc tggaactcgg agacgctgcg ccggcccagg
     3541 tgctggtcga tcatccacag gtgcacgtcc tccatgctct gaaagcgggt gttttgcacc
     3601 gtctggaagg gcaggccatg ctgctggcag atgccgtagc ggttgtggcc gtccaccagc
     3661 acgtcgcccc acagcaccag cgcgtcccgg cagccctcgg ccaggatgct gcgctccagc
     3721 gcgtcatgct cgtcgggcgt gagcgggtcg atataggcct tgagttcggg caggacgacg
     3781 atggtttcgg gattcatggg gtgtcgaaca agcgaaaaaa tggataaagg ccccggcggg
     3841 gacctttgag gcaaaacggt tgatgggtgg taggcatcag atcagaccgc gagccctcac
     3901 ccccaccctc tcccagaggg agagggggta aaaccggcgc aaccggtcgc cctcgcccct
     3961 ttggggagag ggaggggtga gggggtttca ggagggctgc agcagccgcg cgatcaaggc
     4021 cccctccacc gggcctgcca gcggaatgcg cacgcgcacg gggctgccct gggcgacctg
     4081 tactggctcg ccatccaggt tgaacatttt ttccagcagc acctggcgat tgccctgggg
     4141 gtggatgatc tccagccggt cgcccacggc aaagcggttc ttggtttcca cctcggccag
     4201 gccgtcggcg tagccgcgca cctcgccgac aaagtggctg cgttgcagca cgctgtggcc
     4261 ggtttcgtag ttctggtagt cgttggccgg gcggcgctcc aagagacccc cggtgtagcc
     4321 gcgattggcc aggccttcaa gctcggtgat cagctcgggg ttgaagggtc ggccggccac
     4381 ggcgtcgtcg atggcgcggc ggtagacctg ggcggtgcgt gccacgtagt acaggctctt
     4441 ggtgcggccc tcgatcttga gcgaatcgac gcctatctgc accaggcgct gcacatgctc
     4501 cacggcgcgc aggtcgcggc tgttcatgat gtaggtgccg tgctcgtcct ccatgatggg
     4561 catcagctcg ccggggcggt tggcttcttc cagcaggtag acctggtcgg ccttggggtg
     4621 gcgctggata gccgtgccgg tgaactgcgc gtcggcgtcc tcgcgtgact tggtgaagct
     4681 gaaacccttg tccatggtgc tggccagggc ctcgccggtg ttcggatcca cctgggcgtc
     4741 gtgcgtcttg tactcccagc ggcaggcgtt ggtgcaggtg ccctggttgg gatcgcggtg
     4801 gttgaagtag cccgacagca ggcagcggcc ggagtaggcg atgcacagcg cgccgtgcac
     4861 gaacacctcg atctccatgt ccgggcattc ctggcggatc ttctcgatct cgtcgagtgc
     4921 cagttcgcgc gagaggatga tgcgcgccac gcccatcttc tgccagaact tgaccgtggc
     4981 ccagttggtg gtgttggcct gcaccgagag atggatctcg gtctcgggcc acttctcctt
     5041 gaccatcatg atgaggccgg ggtcggccat gatgaaggcg tcgggctggc aggcgatgac
     5101 gggttcgatg tcgcgcagat aggtgcgcac cttgtcgttg tgcgcgatca ggttgctggt
     5161 gacgaagaac ttcttgccgc gcgcgtgcgc ttcctggatg ccctgggcga tctgctccag
     5221 gcggaattca ttgttgcgcg cgcgcaggga atagcgcggc tggccggcat agacggcgtc
     5281 ggcgccgaag tcgtaggccg cgcgcatctt gtccagcgag ccggcgggca gcagcagttc
     5341 gggggctttg agggtcatgg ggggcgattt tcgttcatgc gccatgctgg ggcagggcgc
     5401 gcgggtaatc gctggcgcac gcatcgtccc tggtggatta atggcaaaaa tggccataaa
     5461 cccaggtggg acaagcgcga acagctctct tttttgctga tcaatccttg ggccgaaagc
     5521 tgatcaccag cgacgatggc acgcggccat cgatgtcgcg cggcagggtc tcggtgcgct
     5581 gcagggcgcg gagcacggcc tcgtcccagg ccttgttgcc gctcgattgg gtgatgcgtg
     5641 ctttgactat ggtgccgtcg ggcgcggcga gtacctcgac ctcggcgcgc gggttgccgt
     5701 ccacctgatc cgggaagacg atgttgggct tgaccttggc cgccaccttg ccgccgtagc
     5761 cgcccgaggg gccggcgctg cgcaatgcgg tgccggtggc cgtctcgcca ccggtggcac
     5821 cggccaggcc ctgcatgcgg cggatgttcg cctggtggcg cgcatcggcc tccctggcct
     5881 cgcgcgcttc ctcggccttg cggcgcttgt cctcggccag tttcttctgc ttttcctgct
     5941 cggccttttg ctgctccagc ttcttttgct cggccagctg tttctcgcgc tgctcctttt
     6001 cgcgctcggc cttttcccgc tcggccttct tctgggccag ctggcgttcc ttcttctcct
     6061 gttccaggcg ctcgcggcgt tccttgtcct tcttgtcctg ctccagttcc tgctcgcgct
     6121 ttttcttcag ctcttcctcg cgcttcttct gcttttgcag ggcgatgtcg gcgtcgcgtg
     6181 tgtcgggctc ggcctggcgt ggggcaggcg gaggtggcgg cggtggtgcg ggcgcgggct
     6241 gtggctcggg cgtgggttcg ggggccggcg gcgggggggg cgtttcggcg cgcggcgcgg
     6301 cctgttgcgg cacggcggcc cagagctcgg cctccacggc gggctggtcg ctgctgctct
     6361 tccagttcac gccccaggtc agcgcgacca ccagcacggc atgggccagc accgccaggg
     6421 tgatggcgcg catgcggccc ggcgggcgcg gcggggcgaa ctggtcgcgg tcatgaatgg
     6481 ctgcctgcat gcgcgtggac gatccttggg gttcagccgc cgctcttgac ggccagcgcg
     6541 acgcgcgcca cgccggcgcg ctgcagctcg gacatggcct tcatcaccga gtcgtaggag
     6601 gccgtcttgt cggcgctgat cagcaccggc gtgtcctcgg gctgatcctt gagccaggtc
     6661 ttggcggtgt ggcccagctg cgccagcggc accgtgcgtt cgttgccgtc ggccttgaag
     6721 cgcacgccgc cgtccttgtc cacgatgaca tcggcgcgcg tcttgggcag cttggcaccc
     6781 ttgccgaccg agggcaggtt gaccgaactc ggtgtgagca tgggcgcggt caccatgaag
     6841 atgatgagca gcaccagcat cacgtcgatg aagggcacca tgttgatctc gttcatggag
     6901 cggcggcggc tgccgctgcg ggacgccatt gcgggcatga cgggcctccc ttcaatgtcc
     6961 cgaggggcta ccgggctggg cgcccaggtt gcgctgcagg atgttggaga actcttcgat
     7021 gaaggtctcc tggtgcgtgg ccacgcggtc gatgtcgcgg gcgaagcggt tgtaggcgat
     7081 cacggccgga atcgcggcga acaggccgat ggcggtggcg accagggcct cggcgatgcc
     7141 gggcgcgacg gtggccagcg tgacctgctc catgccggca aagccggtga aggcgtgcat
     7201 gatgccccac accgtgccaa acaggcccac atagggcgac accgaggcca ccgagccgag
     7261 gaaggacagg ctggactcga tcacgtccat ctcgcgctgg aagctcgcgc gcatggcgcg
     7321 gcgggcgccg tcgagcagcg tgccggcgtc actgatgcgg cgctcgcgca gcttttgata
     7381 ttcgcgcatg ccgctggcga agatgcgctc catgggaccg gcgctcttgg cgttgtgcgc
     7441 ggcagcggcg aacagctcgt tcaggctggt gccagaccaa aagtcgcgtt cgaagtcctc
     7501 gttcagagcc ttcacgcgct tcagcgcgaa cagcttgcgg aagatggcgg cccagctggc
     7561 cacggagacg gccagcagca gcagcatcac cagttgcacc acccagctgg cgcccagcac
     7621 caggctgagc atggacatgt cttgggaatt catgatcggg aaattgcttg caaaacagaa
     7681 tcagggatgc gcgccgcgcg catgctggtg ccatcgaccc agcctatgcg tatgctggct
     7741 tcgcacagca actggggctg ggctggcgtg gcttgtgttg tcaggaaggc ctgttgttcg
     7801 attgtcatgg atgcacggcc actgtcctgg agcctggccg taacaatcag ttcatcgtcc
     7861 agccgggccg ggcgcaggta gcgcaggcgc gcgtcggtta cgacaaacat gccgccggtt
     7921 tgttcccgca actgctgctg gcccaaatcc agcgcgcgca gccactcggt gcgcgcgcgc
     7981 tcgaagaact tgaggtagtt ggcgtagaac acgatgccgc cggcatcggt gtcctcccag
     8041 tagatgcgta ccggccactc gaaagccatg ctgcgtcagc cgatcaaacg ctgcaaacgg
     8101 ctgaccgcct cctgcagctg ggccatggag tttgcggtgg aaaaacgcac atggcgcgcc
     8161 gtgtcggccg tgccgaagtc gcgccccggc gtgatggcca gatgggcgcg gcgcatgagc
     8221 tcaaacgcga agtcccagct gccatcgaca cccagcctgt gcgccacctg cgtgcagtcg
     8281 gcccaggcat agaaggcgcc gtcgggcatg acgggcacgc tcaatccgag cttgttgagt
     8341 tcgggaatga agtagtcgcg ccgcgccttg aactcggcgc gccggcgctc gtactcggcg
     8401 atgctctctg cctcgaagca ggccagggcg gcgtgttgcg agatggtgga ggcgcagatg
     8461 aacaggtttt gcgccaggcg ctcgaccacc ggcaccatgg catccggcac caccatccag
     8521 cccaggcgcc agccggtcat gttgaagtac ttgctgaagc tgttgatgct gatgatgtcg
     8581 tcgcccagcg ccagtgcgct gtggctgtac tgctcttcat acgacaggcc caggtagatc
     8641 tcatcgatga tggtcaggcc gcccttggcg cgcaccacct ggtgtatggc gcgcagctca
     8701 tcgggggcga tcgaagtgcc cgtggggttg gaaggcgagg ccagcaaaac gccgcgcgtt
     8761 ttttctcccc acgccgcttc gaccttggcg gcgctgagct gaaagcgttc ctcggcggtg
     8821 gtcgggatca gcaccgcctt gccctcggcc gcgctcacga agtggcggtt gcaggggtag
     8881 ctggggtcgg gcatcaggac ctcgtcgccg gcgtcgatca gcgccaggca ggccagctgc
     8941 agcgcggccg aggccccggc cgtgaccacg atgcgccgcg ccggcacatc gacgccaaag
     9001 cgcgtggcgt accagtcgct gatgcgctcg cgcagcggct ccaggcccag ggcgttggtg
     9061 tactgcgtca ggccgtcgcg tatcgcgcgc tcggccgcct gttgcacgag cggcggggcg
     9121 gtgaaatccg gctcgccgat gttcaggaag atcatcggct cgtgcgtgcc ggccacttcg
     9181 cgcgccattg cctgcgcggc cttggccact tccatcacgt aaaagggttc tatgcgctcg
     9241 gcgcgggtgg agaacttcat gacactgtgc ctgccgccgc ccagggcgca cgggcttgag
     9301 agaaacaaaa ggggctcagc tggccttgcc ggcggccttg tcggcctcga cctcggcggc
     9361 gcgcagctgc ggcgccaggc cgttcaagac gccgttgacg tacttgtggc catcggtgcc
     9421 gccgaattcc ttggccagct cgatgcactc gttgatgacc acgcgccagg gcacgtcggg
     9481 gcagtgctgg aactcgtaca cgcctatcca catcacggca tgctctatgg gcgagatctc
     9541 ggccagcttg cggtcgagct gcggcgtgat cagctcgtcc atgaactggg cggtggtgat
     9601 gcagccgtgc aagagcgcgt cgtaatgcgc ggcatcggcc ttgtgaaagc ctgacaggtc
     9661 gcgcgtgaac aggtcgatgg agctggcgtc gttgccgccc accagatgct ggtacagcgc
     9721 ctgcagggca aattcacggg cacgcgagcg ggccgacttg gagccggcct tgcgcgcgcc
     9781 tgtgctggtg gtgccggtgc gcgactggcg cggggggcgc ttggacgcgg gggcctcgct
     9841 cactgcagct cctccagcag cctggccatc tccacggcca cgcgggcggc gtccacgccc
     9901 ttgtcggttt ggcgcgcgat ggcctgctcc aggttctcgg tggtcaggat ggcgttggcg
     9961 atcgggatct ggtagtcaag ccccacgcgc gtcacgcccg cgccggactc gttggccacc
    10021 agttcgaagt ggtaggtctc gccgcggatg atgcagccca gggcgatcag cgcgtcgtac
    10081 tcatcgcgct cggccatggc ctgcagcgcc agcggcacct ccagtgcgcc gggcaccagc
    10141 acatggtgga tatgcgcctc ctgcacgccc agcttgatca gctcggcgcg gcaggccgcg
    10201 gccagggtgt tggtgatgcc ttcgttgaag cgggcctgaa ccaggccgat gtgcagttcg
    10261 ctgccgttga gctgcgccgc cgtgcctttg tctgcgtcaa acatgtctta ttccttgggg
    10321 atgtagccgg tgatttccaa accgtagcct gtcatgctgg gcatgcgccg gggctggccc
    10381 atgagctgca tgcgcgccac gccgacctcg cgcaggatct gcgcgcccac gccgtaggtg
    10441 cgcaagtcca tgcggccgcg ctcgggcgcc tgggcggcgc gcgccttgcc ctcgaactgc
    10501 tcgagcagtt gctcgccggt ttcgccgcaa ttgagcagca cggccacgcc gcggccctgg
    10561 gcggcgatgt ggcgcaggct ggcgtccagg ctccaggaat gcatggcgcg gctgggctcc
    10621 agcgtgtcga gcaccgacag cggctcgtgc acgcgcaccg gcacgctgtc ggccgcatcc
    10681 cactgtccca tgaccagcgc caggtgcacc gcctggctgg gttcgtcgcg gaaggcatgg
    10741 gcggtgaact caccaaacgc cgtctgcatg gggcgcgagc ccaccttgtg caccagcgac
    10801 tcggtgcggc tgcggtattc gatcaggtcg gcgatggtgc caatcttcag gccatgctcg
    10861 gcggcaaaca gctgcaggtc gggcaggcgg gccatggtcc cgtcgtcctt catcacctcg
    10921 cagatcacgg aagacgggct gcagccggcc atggcggcca ggtcgcagcc ggcttcggtg
    10981 tggccggcgc gcatcagcac gccgccatcg accgcctgca gcgggaagat atggccgggc
    11041 tgcaccaggt cggcagcctg ggcattgggt gccacggcgg cctgcacggt gcgcgcgcgg
    11101 tcggcggcgc tgatgccggt ggtcacgccc tcggcggcct cgatggagac ggtgaaggcg
    11161 gtgcccatct tggtgccgtt gcgcgccacc atgggcggca gttgcaggcg ctcgcagcgc
    11221 tcgcgcgaga gggtcaggca gatcaggccg cggccgaagc gggccatgaa gttgatggcc
    11281 tcgggcgtga catggtcggc ggcgatgacc agatcgcctt cgttctcgcg gtcttcctca
    11341 tcgacgagga tgacgatgcg cccggcgcgc atctcggcca cgatgtcttg cacgggcgag
    11401 atgggggcgg gcgtgagggg ggtgttcatg cggggccttt gcgtagtccg tgcgccctgg
    11461 ggcagcgcgc gttccccggc gggtgggccc caaagaacgg cgatgcggcc gcgggccgca
    11521 cgggtgaggg atgcggtgat cccgaaagac ggcgatttta gtccacggcg tcagatggcg
    11581 gaggcttcga gccggatgtc gctcatcgga taggccacgc agggcagcac gcagccctcg
    11641 gccttttcct cgggcgacag gccgggccag tcgatggcgt agcgcacctc gccgtcggcc
    11701 agcatgccga tgcaggtgcg gcagctgccg ttgcggcagg agctgggcca gtcgatgccg
    11761 ccttgctcca gcgacagcag caggggctgc tcgggccagg catcgcactg ggtgccgtca
    11821 tcgcccacgc gggcaatgaa aaagggtggg gtcgtgtttt ccatagaaaa tgccttcaac
    11881 gcttgtcggg aaagcgcaaa tagctatcaa aataatagta aaaagaatct aagggagctg
    11941 cctaaaaatt gggcattgca ggtctgggca ttgcaaatca ggcacttaag accgccatac
    12001 agcgcttgtc caaagttatc cccaggattg tccaatggcc tgtggaacaa ctcctggcca
    12061 ggctgcctaa aaattcatca tcgcaagcaa aatcattaca aatcaaatac ttgcaatcgc
    12121 agtacagcgg ttgttcaggc ttatccacat gtttgtccag tgccaccagg gataagctgg
    12181 ctcagggttc gcgcgcatct tcagtcgcgg tgcggctcag gttggctggc accgcctcgc
    12241 ccatgcggcg cgcgcggaac agcgcggcac gcatcaggaa gatgttggtc acggggacgg
    12301 tgacggccac gaacagggcg atcagcagcg catgcacggc caggccttcg cccaccatgg
    12361 agaagtacag cagcgtggca tgcatgacgc accagcaggc catggtggca atgatggccg
    12421 gggcatgcac gcgctcgaaa tagcttttca ggcgcagcag cccgagcgag cccagaaagg
    12481 caatcgcggc accggccagc accagcagcg agacgctgat gtccagccac aggggcagat
    12541 cctggccatt cactcgatca cctcgccgcg cagcaggaac ttggccatgg ccgtggaact
    12601 ggcaaaaccc accagggcga tgagcatggc ggcctcgaag taatgctgac tgccgtagta
    12661 gatgcccagc accagcatca gcagcatgcc gttcaaatag atgcagtcca gcgccagcac
    12721 cctgtcctgc gcggccgggc ccttgagcag gcgcaccagg cacagcacca tggccgcaac
    12781 gagcataaac agggcgctgg gcaaggccca cgcaagcagg gagccggtca ttcgaagatc
    12841 tccatcaggg ggcgctcgta gcgcgtctgt acatgctgca cgatggcctc gcggtcatgt
    12901 acctccagca catgcagcat gagcatggag cggtcgcgcg agagctcggc ccagaccgtg
    12961 ccgggcgtga tgcagacgat catggccagt accgccaggc cgttggggtc gcgcagctca
    13021 agcggtatct tcacgaagtc ggcgctgtgg cggcgcgtgc gcggcagcag cagaaagcgc
    13081 agcacggcga tgttggactg ggtcgtgtcc accaccaccg tccagcacag ccgcagcacc
    13141 gtgcccaggt ggcgcacgcg caccttttgc gggcgcagct ccttgaacag caccggcaac
    13201 cccagggcca gcagcagggc cagcagcagg tggccggggc tgagcgagcg gttgagcagc
    13261 agccagacca caaacagccc gaacgagacc agcggtgcgg gcaggatttt cttcatcatg
    13321 gccgggcctc ctttccggcg ggccgggcac tgtcgccggg gccggtccgc ggcggtgcgt
    13381 tcagcacggc acgcacatag ccggcggggg cgtacagcgc ccgggccgtg gccgtggtga
    13441 agcgcatcac gccctcggcc tggacggtca gtaccacgca ggcggccagc agcaacgcga
    13501 tgggaaggcc ttcgagcaca tgcagctgcg gcctgtccct ctccgaggcg gcccagaaat
    13561 ggcggatccc ggaccgtgtc agcgccatca gagccaccag gcccgtgcca atcatcagcg
    13621 ccagcagcgt ccagccggcc ggcccggggc tggtacccgg cgcggtgccc aggccctgcg
    13681 gatcgagcag cgggccgagc atggcgaact tgccgatgaa gcccgacaac ggcggcagcc
    13741 cggcgatcac cagggtgctc atcagatagg ccaggcccat gaaggcggtg gcggccggga
    13801 tcacccggcc gaccagcacc tgttcttcgt catccaggtt cagaccggcg gtgggcacga
    13861 gttctgccga aagaaagggc gcgtcgtcgt cgttttcgta cggggccagg gtggcgccgt
    13921 cgttgcgcca gcgctcggtc aggtcggcca gcaggaacag cgccgccacg gccagcgtgg
    13981 agctgggcag gtagtacagc aggccggccg tgagccgatc ctggccaaag ccggctgcgg
    14041 ccagcagcgt gcccgacgac agcacggccg cgtggctggc caggtgcgtc aggcgctgcg
    14101 agcccatcat gccgatggcg ccaaaggcca tggtggccat gccgcccagg atcagccaca
    14161 ggccgccaaa ctgtgccgag gccccggcat cggcgccaaa cagcagcgac cacaggcgca
    14221 gcagcgcgta cagcccgacc ttggtcatga gcgcaaacag cgcccccacc ggggccgtgg
    14281 ccgcgctgta ggcgggcacc agccagaagt tcagcggcca cagggccgcc ttgatcagaa
    14341 aggccgtggc caatatggcg gccgccgcgt gcagcaggcc ccggtcggcc ggcaacacct
    14401 ggggcacggc ccgggccagg tcggccatgt tcagcgtgcc ggtgagggcg tagagcatgg
    14461 ccgcgccgac caggaacagc gacgaggccg ccaggttgat ggcgatgtag tgcaggccag
    14521 actgcacgcg cgcgcgcccc gagccatgca gcagcaggcc gtaggaggcg gccagcatga
    14581 tctcgaagaa cacgaacagg ttgaacaggt cgccggtgag gaacgctccg gccaggccca
    14641 tcagctggaa ctggaacagc gcctggtaga gcacgccggc acgctgccag cgcgcgcccg
    14701 cgaacagcac cgaacacagg gcgaccaggc tggtcagcag cagcatcagc gccgtcagcc
    14761 ggtcgagcac cagcacgatg ccaaacgggg ccggccagtt gcccggcagg tacacgccca
    14821 tgcccacctc tgcgccctgg cggtgcaccc attgcagcaa ggcaatcgac accagcaggc
    14881 ccaccagcgt ggacaccagg ctgacgccga acttcaggcg ctgctgctcc tcgcgcagca
    14941 gcaacaacag cgcggccgtg aacatgggca gcgcgatggg cgccagcatc aggtgcggca
    15001 tgagcgcgct ggtgacctca gacagccggc tcatggcatc tcctgcgcat cgcgcgcctg
    15061 cgagccgtct acatgatcgg tgccggatat gccgcgtgcg gccagcagca ccaccaggaa
    15121 cagcgccgtc atggcaaagc cgatgacgat ggcggtgagt accagggcct ggggcagggg
    15181 gtcggcgtaa tgctgcagat cctgcgccac gccgggtagc agcaccggct ccttggccag
    15241 ggccaggccc aggcggccca tgctgaagat gaacaggttc accgcatagg acaacaggga
    15301 caggcccatg atgacctgga aggtgcgcgg gcgcagtagc agccacaccc ccgagccggt
    15361 gagcacgccg atggagatgg cgaggatgat ttccattcag cgcgcctccc tggcgtcgtc
    15421 gtcatagctg accacggccg ccatggccag ctgctgctct tccagcaggc gcgcgtggta
    15481 gcggtggctg cgcaccgact ggtgcgcaat ggccgtgagc atcatcagcg tggagcccac
    15541 caccagcgcg aacacgccgg catcgaagaa catggcgctg gccagatgca cctggccgag
    15601 cagcggcaac tggaggtgga acatatggct ggtcaggaag gggtagccca cggccacggc
    15661 gcccaggccc gtgcccagcg ccagcagcag gccggtggcg atccagcggc gcgggaaaac
    15721 cggcaggtgc gcctccaccc attccgtgcc cgagatcatg tactgcagca ccaggcccac
    15781 ggagaacacc aggccggcga cgaagccgcc gcccggctgg tcatggccgc gcaggaacag
    15841 atagcaggcc accagggccg tgaacggcag cagcaggcgc accagcacgg ccggcaccat
    15901 caggtagccc acggccgtgt cctgcgcgtg gcgtgggttc agcaggtcgg tctgcaggtc
    15961 ggcgggcagg ttgcgctgct gctcgggcag atccatggcc tcgccggcgg ggcgaaagcg
    16021 ccgcagcagt gcatacacgg tgagggcaac gatgcccagc accacgatct cgccaaaggt
    16081 gtcgaagccg cggaagtcca ccagcatcac gttgaccaca ttggtgccac caccttcggt
    16141 cagcgcgcgc tccaggaaga aggtggaggt gctttgcgca aacggccggc tcagcatggc
    16201 aaatgacagc caggccatgc cgccgccggc cgccagcgcc agcagtagat cgcgcaggcg
    16261 gcgcacgcgg gtcagcgcgg tgctggccga tgccgcgggc aggctttcgt cgcgcttggg
    16321 cagccagcgc aggcccagca ggatgaggat ggtggtcacc acctccaccg ccagctgcgt
    16381 gagcgccaga tccggcgccg agaaccatag aaaggtgatg ctggtgcaca ggcccgcgcc
    16441 gcccatcagc gccagcgccg ccagccggtg gtacttggcc tgccaggccg ccgccacggc
    16501 gcacacattg cccagtagcc acaccagcac aaacgccggc gatagcgcca gcgcggcgcg
    16561 cccgcccagc cgcatgctgc ccagccacag tggcagggcg ccggccacca gggtgaacag
    16621 cagcagccac agcatctgcc actgcagccg cctggtggac agcaggcgcc ggcccttgcg
    16681 cgcggcctgg gtgcacttgg cgagcagggc ttcaaagacg cgcctgccgc tgatgaagcg
    16741 catcagcggc ggccctccga tgcggccgag tcggcgcagg ctgcgcagcg cctggtacag
    16801 cgcggcgcca ccgatgaggg cgacaaagct catcaacagg ggcgtgttca ggccatgcca
    16861 tagcgccagg ctgaaggccg gcagctggcc gcccaccacg ggcagcgcgg cggcatccag
    16921 aaaacggcct atcgaccagg ctggcagcac gcccaccacc aggcaggcga gcaccaagag
    16981 ctccaccggc acgcgcatcc agtggggcgg ctcatgcggc gcgtagggca ggtcggttgc
    17041 cggcgggccc cagaacacat cgacgatgaa gcgcagcgaa taggccacgc tgaacatgcc
    17101 ggccaccgtg gcgcccaccg gcaacagtgt ggagaccagg ggcgaggcgt ccaggaacac
    17161 ggtctcggcg aagaacattt ccttggacag aaaaccgttg agcagtggca cgccagccat
    17221 ggcggcgctg gccaccgttg ccagcgtggc ggtgatgggc atcatgcggc gcaggcccga
    17281 caggcggttg atgtcgcgtg tgccgctttc atggtcgatg atgcccgcgg ccatgaagag
    17341 cgacgccttg aaggtcgcgt ggttcatgat gtggaacacg gcggccacgg cggccagcgg
    17401 gctgttcagg cccagcagca gcgtgatcag gcccaggtgc gagatggtgg agtaggccag
    17461 cagccccttc agatcgtcct ggaacatggc ggcgtagccg ccgatcagca gcgtggccag
    17521 gccggtgccg cccaccagcc agaaccaggc ctcggtatca cccagcaccg gccacaggcg
    17581 cgccagcaga aacactcccg ccttgaccat ggtggccgag tgcaggtagc tcgataccgg
    17641 cgtgggcgcc gccatggcgt tgggcagcca gaactggaag gggaactgcg cgctcttggt
    17701 aaacgccccg agcagcacca gcaccagggt ggccaggtag agcggatgtg cacgcaccag
    17761 atcgcccgcc gccagcacct ggtcgaggtc gtagctgccg acgatatggc ccaggatgag
    17821 catgccggcc agcaggcaca ggccgcccgt gcccgtcact gttagtgcca tgcgcgcgcc
    17881 gcggcgcgca tcctcgcggt gatgccagta gccgatcagc aggaaggaaa acaggctggt
    17941 cagctcccag aaaaacacca gctggatgat gttgcccgac agcaccaccc ccaccatggc
    18001 gcccatgaag gccaggaaga aggagaaaaa gcgcggcacc gggtcttcgg gcgacatgta
    18061 gtagcgcgcg tacagcacca ccagcgcgcc tatgccgaac accagcatgc agaacatcca
    18121 ggcaaagccg tccaggcgca ggatcaggtt catgcccagg gtgggaagcc aggcaatctc
    18181 ctggcgcagc acgccgccgc cggcaatgtc gggaaagctc aaggccgtca gcagcgcgca
    18241 gagcagcgcg atcacccccg ccagcgtgga ctcggcattg cgcgcgttgg ctggcaacag
    18301 ggccgccagc aggctggcaa gaaaggggag ggcgacgagc agaaccaggg acatgagcgc
    18361 ctgattctag gggtaaacgg ttttcaagat gctatggata cttgagcggt agccggccat
    18421 gggatcgggc ctggccgtgc catcaagctc aatcgcggga atccgttcgt gctggcggcg
    18481 ctgtctgcgg cgctgcatag ggtgcaccgt tgaccgtgag cccgttgtca gacaggcgtg
    18541 cagcggttgc agggcctatg cccttgacac gggcaatcag atccggccag tcctgaaagc
    18601 gctgcctggc acgctcgacc aggatgcgcg cggtcagcgc cgggcccagt ccctggatgc
    18661 cgtccagatc ggcctcgcgc gcctggttga tgtccgtgcc ggccagcgcg gcgcccaggt
    18721 gcagcagggc ggcaagacac aggctgtggc gcaggcgcat ggcggctcct acagctcttc
    18781 cacgcggcgc gcggggtagc tgtcccagct ttggcagccc gggcattgcc agaaatgctg
    18841 gcgcgcctcg aagccgcagg cggcgcagcg gtagcgcgtg agcggcttgg tggcctgctc
    18901 cagcgcgcgc tggatctgcg ggtggaactg ctcctggctg agggtctcgc cagccagcca
    18961 tttgctggcg gccaccagcg acggctcctt gtccaggtgg cgcatatacc atgcgcgagc
    19021 ggcagtggcc gagccctgcg tggcggcttc cagcgtcacc acggcttcga gcagatccag
    19081 ggtcggcatg tcctcgtaat gctggcgcag cagggcgccg gcctgttcgg cctggccgct
    19141 ggcaagtgcc gcctccacca gcagcggcgc cgccaggccc agggccgccg gggtggtctg
    19201 ggccagggtt tgcagcgttg acagggctgc gtcggcctgg cccatgcgtt gctgcaggcg
    19261 cgccagctcg atgcgcgggc gtgccgcctg gggggctgtg cttgcagcct gcagcagcag
    19321 ggtctgggcg gcaggcagtt cgccccgggc gctgagcgcc agggcctgtt cgcacaggta
    19381 gtgggcctgg cgcgtactga aatcgccctg accggcgcct tgcatgcgct gtgtgatgtc
    19441 ggacgcctgc tgccagtcgc gcgaacgttc gtagatggcc agcagcgcca tgcgcgcctg
    19501 ggcctcgaag ggcgtgccct ccaggcggcg cagcgcgtcc tcggcgcggt cgagcaggcc
    19561 ggccttgagg aagtccagcg ccagcgcatg ctgggcacgc tcgcggtcgg cgcgcgagag
    19621 gtcgccacgc gagagcaggt gctcgtgcac gcgcacggcg cggttgtatt cgccgcggcg
    19681 gcgaaacagg ttgcccagcg caaagtgcag ctcggtggtg tccgggtcgt tctgtacggc
    19741 ctcgataaag gcgtcgatgg cctgatcctg ctgctcgttg agcaggtagt tcagaccctt
    19801 gaaatatgcc ttgggtgcgc gccggttttc ctcgcgcagc tggcgcagat ccaggcgcga
    19861 ggccagccag cccaggccaa aggccagcgg cagtccgagc agcagccagg tgaagtcaaa
    19921 ttccatcgat gggcagatgt ggggctacgg gctctgccgc gggggcagga ggcgcggggg
    19981 cggcgctggg tgggggcgct tcggcctggg cctggcgtgc ggcgctgcgg tggcgccacc
    20041 agcgcggcac catgcccaga acgcccacga ccaccccgag cgcaaatgcc gacagcacga
    20101 tcagcacggc cggcgccgtc cactgggtgc caaagaaaaa atgcaccgtg gcgtcttgct
    20161 gattgttcag tgcgaaggca aagagggtaa aaaaaatggc tgccttgagc agccacagga
    20221 ggtatttcat gcatctcccc agtggtgggc gagattctac gggtagcgtc acggcatcgg
    20281 aacggcttgt gcaatgcaag ataaaaatat ggctctagcg cttgctggat aagcgcaggc
    20341 agctacattt tttgatgttt gtggttcagg aggcctgggg cggttggtcg acggactcgc
    20401 gcagggcctt gccgggcttg aaatgcggta cgcgcttttc tgggatctgc accgcctcgc
    20461 cgctgcgcgg gttgcggccc agccgtggcg gtcggtgact caccgagaag ctgccaaagc
    20521 cgcggatctc gatgcgatgt ccgcgcacca gggcgtcgcc cacggcgtcc agaatggtct
    20581 tgacggcggt ttcggcgtcg cgctgggtca gctgcttaaa gcgtgcggcc agttcttcga
    20641 caagatcgga tcgggtcatg gtgtctaggc aggtttaaaa aacaacgggc gctcatcggc
    20701 gcccgtcatg ttagccgctg gcgtgcaggc cagcggctcg ctccagggat tacttgtcgg
    20761 agttgtccag cttggcgcgc agcagggcgc ccaggctggt cgtgccggca ttttccttgg
    20821 cggactgggc cgacaggtga gccatggctt cctgctggtc ggcctggtcc ttggccttga
    20881 tggacagctg gatgttgcgg gtcttgcgat ccacattcac caccacggcc gtcacttcgt
    20941 cgccttcctt gagcacgttg cgggcgtctt ccacgcggtc gcgggagatt tccgatgcgc
    21001 gcaggtagcc gacgatgtct tcacccagat cgatctcggc gccgcgggcg tccacggtct
    21061 tgaccttgcc ggtcaccgtc tg
//
LOCUS       DAOMTQ010000035        21055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_297_length_21055_cov_26.851238, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000035 DAOMTQ010000000
VERSION     DAOMTQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 21055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21055
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_297_length_21055_cov_26.851238"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /gene="mgtA"
                     /locus_tag="PK518_05375"
     CDS             <1..1086
                     /gene="mgtA"
                     /locus_tag="PK518_05375"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HRM48377.1"
                     /translation="IGYVAFLDPPKDSTAAALKALAEHQVAVKVLTGDNELVAAKICR
                     EVGLAQQGVLLGRDIEAMNDQQLGEAVENHNLFAKLTPLHKERIVHRLKANGHVVGFM
                     GDGINDAPALRAADIGISVDSAVDIAKEAADIILLEKSLMVLEQGVQEGRRTFANMLK
                     YIKITASSNFGNVFSVLIAAALLPFLPMLPMHLLVLNLLYDISQIAIPFDNVDEEMLR
                     QPLRWQPGDIGRFMVFFGPLSSVFDMLTFAMMWWVFGAQTPAQQTLFQSGWFVVSLLT
                     QALIVHLIRSPKLPFIESRASLPLMAMTLAIVTVGLFLPMGPLAHYFKLQALPPLYFA
                     LLPLTMLGYMALTQAMKGFYRRRWGWQ"
     gene            1124..2782
                     /locus_tag="PK518_05380"
     CDS             1124..2782
                     /locus_tag="PK518_05380"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRM48378.1"
                     /translation="MDWTTHQVFNQFPELGDYDPLASDPALCEALARADAGWARPALD
                     SYARRLGRAETYTLAQQANRHAPELHRFDTRGRRIDAIEFHPAWHQLMALYRGQGLVS
                     LPFREQQPGRWTAWAAGFYLHGQVEQGTLCPATMTQAAIPLLQREDALWAQLKDKLYS
                     DTYDARDMPAADKAGIWLGMGMTEKQGGSDVRANTTVATAMGAGGRGGEYLLRGHKWF
                     FSAPQCDAHLVTARVGADGPFACFYVPRWRPDGSRNAVRVQRLKDKLGNRSNASAEVE
                     FEDAWGILIGEEGRGIPTIIEMAGYTRLNCVVGSAAILRQATVQAIAYARRRQAFGKA
                     LADQPLMRAVLTDLALESEAALQLAMRLAQACEDATSSPGERAWRRIMTPAAKFWVCK
                     RAVELTGEAMEVLGGNGYVEDGVLARLYREAPVNSIWEGSGNVMCLDVLRALQRDGDA
                     AQALLAELQSAAAGEPRLAVALRELLALLALPPEQQQALGRLLAQRLVLLAQACLLRR
                     HAPSAMADAFIATRLADGGAGRVAGAMDMRGLDVAAILARAFPA"
     gene            2861..4060
                     /locus_tag="PK518_05385"
     CDS             2861..4060
                     /locus_tag="PK518_05385"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRM48379.1"
                     /translation="MLTLQEIQDAAARLSGQVLDTPCVESRTLSQIVGAQVFLKFENL
                     QFTASFKERGACNKLAQLTAEERAHGVVAMSAGNHAQGVAYHAQRLGLRAVIVMPRFT
                     PGVKVERTRGFGAEVVLHGDTLEEARQHAYQLAEQQQLCFVHPYDDEAIAAGQGTIAL
                     EMLAEQPDLDALVIAVGGGGLLAGIATAARALKPGLDIVGVQAARFPSMVNAIQGTQH
                     PQGQSTIAEGIAVGQPGEMTREVISRLVDDLLLVQEGDIEQAVLMLLEIEKTLVEGAG
                     AAGLAALLRYPERFRGKKVGLVLSGGNIDPLLLAAIIERGMVRSGRLARILVSARDIP
                     GVLARITATVAEAGANIEEVHHQRAFTMLAAQNVEIELVLQTRGKAHVQQVLDQLRAA
                     HMEAELR"
     gene            4152..4310
                     /locus_tag="PK518_05390"
     CDS             4152..4310
                     /locus_tag="PK518_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48380.1"
                     /translation="MTHRPIAPNAADVEVATATDPIETVVDVIPFVIPAAGALVIFLL
                     AFIAVSMG"
     gene            4630..6495
                     /locus_tag="PK518_05395"
     CDS             4630..6495
                     /locus_tag="PK518_05395"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRM48381.1"
                     /translation="MNAPTLQGLTIQAPAYVKNAKLIAWVAEMAALCKPARIHWCDGS
                     QEEYDQLCQQLVDAGTFKKLNPAKRPGSYLAWSDPSDVARVEDRTYICSEKKEDAGPT
                     NNWMAPNEMRATLAPLFDGCMAGRTMYVVPFSMGPLGSPIAHVGVELSDSAYVAVNMK
                     IMTRMGRAVYDVIGADGEFVPCMHTVGAPLAAGQKDETSWPCNAKTKYIVHYPETREI
                     WSYGSGYGGNALLGKKCLALRIASTMGRDQGWLAEHMLILGVSNPEGKKYHVAAAFPS
                     ACGKTNFSMLVPPQAFNGWKVTTIGDDIAWIKPQADGSLRAINPEAGYFGVAPGTNYH
                     TNPNCMASLDKDVIFTNVALTDDGDVWWEGMEKDTGVMPAHLIDWQGKDWTPEDGKAG
                     RKAAHANARFTVAATNNPALDEAWDDPAGVKIDAFIFGGRRSTTVPLVTEARNWTEGV
                     YMAATMGSETTAAAFGAQGVVRRDPFAMLPFMGYNMSDYFQHWLNLGAKISAQGAQLP
                     RIYTTNWFRKNAEGKFVWPGYGENMRVLKWMIDRIEGQAQGQETAFGIAPQYAEINWG
                     GLDFSAEQFQNVTSIDKAAWIDELKLHDEHFAMLAQGLPRELLNAKADLEQRLSA"
     gene            complement(6634..7074)
                     /locus_tag="PK518_05400"
     CDS             complement(6634..7074)
                     /locus_tag="PK518_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48382.1"
                     /translation="MTTSASIELYTQHRGLARMRGAARYLRQAAARSSGARGATSLAL
                     AATVAALMVAANTLIDSWSDGHLLAAWLVLWGIAFAGMALLAGPVRKLVSLVLSGTEA
                     WTEAYRRAQEDEKTWNAALHDARILADLSRAMNGIAVEDMRRYR"
     gene            7225..8070
                     /locus_tag="PK518_05405"
     CDS             7225..8070
                     /locus_tag="PK518_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM48383.1"
                     /translation="MKLQLLSDLHLEIHPHFRPSPAPGADLLVLAGDIGSYQPGSLLA
                     DDDFGLARFSPLPQYAGWPTPVVFVPGNHEYDGQDFDAAHARLRHVCDRLGLIWLERE
                     SLVLDGVRLVGTTLWSDFDALADHHGITDLGQRLKRREKAFRAADFYLAKTGGTRRGE
                     PFLAAQLREQGLACQAWLRQALSQPFDGTTVVITHFAPSLRSADPRYGLTPGTAGFCN
                     AMDELLPRARLWLHGHLHAPSDYLAQGVHGDGRAWHCRVVANPLGYARKGEQDAFLPQ
                     CVLTV"
     gene            8161..8583
                     /locus_tag="PK518_05410"
     CDS             8161..8583
                     /locus_tag="PK518_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM48384.1"
                     /translation="MNILLAVDGSPYTKKMLAYLAAHDEMLGTSHSYTAITVQAALPP
                     RARAALGKETVDSYYAEEGEKVLAPVVKFLARHGVQLKTVTKVGPAGETIAKVAESGK
                     YDLIVMGTHGHGSLGKLVMGSVSTQVLANCQVPVLLVR"
     gene            complement(8743..9378)
                     /locus_tag="PK518_05415"
     CDS             complement(8743..9378)
                     /locus_tag="PK518_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM48385.1"
                     /translation="MNMLSQPRPSFKAQMHQAREEAIVEATSRLLGIKGFETMTVDEV
                     AATVGIAKASLYKHFAGKDDLCAAAMVHGVGRLQRFLAELSAELSPLQRLQALLHWLL
                     ELQLGDDTPLLPERNSALAQALRGCDVYHASMQEVSACILGWIVQAQAAGQLRRELPP
                     DVVLCALLARSADPMLAMLRERGYGNAEILAWAMDTCLSGLVPQPAAELRA"
     gene            complement(9452..10234)
                     /locus_tag="PK518_05420"
     CDS             complement(9452..10234)
                     /locus_tag="PK518_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM48386.1"
                     /translation="MSLLPTFSLLGSGPTVLLLHDADGDHLSFAPQLETLAAAGYRAV
                     AWDMPGYGRSAPVEPYGFKGLAQRCLLLLDALQCDEATLVGHGLGAMLALELALRAPL
                     RVRRLVLCAGGPPLDAAAIAAWVAPRLEALDEGLSMSELAARLVVHAAGDAALPEGLH
                     LARHAMGRVHAATYRRALQALASFARRAPELMRLHQPTLLIGGEQDRCTPPEALQALA
                     HVLRDARLVQLRGVGHWPQLEDPEGFEAELLDFLAEPDQARH"
     gene            10319..10885
                     /locus_tag="PK518_05425"
     CDS             10319..10885
                     /locus_tag="PK518_05425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRM48387.1"
                     /translation="MSFVNSSLPRTAAHPPQFSPREFRDALGMFATGVAIVTARSENG
                     QPLGMTINSFNSVSLEPPLVLWSLAQRSSLLQALTLTRHCAIHVLTAAQRPLAERFAT
                     RGIDRWAGVEHQLGLQGVPVIAGAAAVFECHVRSQYAEGDHTIFVGEVLRCGHRQGLS
                     PLLYHGGMFYTEHALGRLSPKATGPMDR"
     gene            complement(10904..11167)
                     /gene="minE"
                     /locus_tag="PK518_05430"
     CDS             complement(10904..11167)
                     /gene="minE"
                     /locus_tag="PK518_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRM48388.1"
                     /translation="MSLLSFLLGEKKKTAAVAKERLQIILAHERSGRSAGKPDYLPAL
                     QRELVAVISKYVKINPEDLKVHFERQDDLEVLEVKIELPEAAK"
     gene            complement(11174..11989)
                     /gene="minD"
                     /locus_tag="PK518_05435"
     CDS             complement(11174..11989)
                     /gene="minD"
                     /locus_tag="PK518_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRM48389.1"
                     /translation="MAKIVVVTSGKGGVGKTTTSAAFASGLALAGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEANLNQALIKDKQCENLFVLAASQTRDKEALTQDGV
                     GKVLKDLAEMGFDYIVCDSPAGIESGALMAMHFADEALLVTNPEVSSVRDSDRILGML
                     SSKTKRAIEGSEPIKEHLLITRYNPNRVEDGQMLGLEDIQDILRIKLIGVIPESESVL
                     QASNQGLPAIHLGGTDVSEAYKDVVARFLGEDKPLRFTEAVKPGFFKRIFGGR"
     gene            complement(12046..12795)
                     /gene="minC"
                     /locus_tag="PK518_05440"
     CDS             complement(12046..12795)
                     /gene="minC"
                     /locus_tag="PK518_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRM48390.1"
                     /translation="MAVDTAGQARTPLDLKSAQLSVVAMVLKTVDVAAVAAELAARSA
                     DDPEFFDNDPVLIDLSAVQAEEQPIDFAALVQLLRSHRTQPVAVRGGNPAQMQAAHAA
                     GLVAAPDMPAARAQPREVIREVVREVQVPVEVPVPAPAPGALVVDKPLRSGQQVYARG
                     SDLVVLAMVSFGAEVIADGNIHVYAPLRGRAIAGARGDTSARIFSTCLEPQLVSVAGI
                     YRTTETELPADVRGNPAQVRLDGEKLLIEPL"
     gene            complement(12888..13511)
                     /gene="pssA"
                     /locus_tag="PK518_05445"
     CDS             complement(12888..13511)
                     /gene="pssA"
                     /locus_tag="PK518_05445"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRM48391.1"
                     /translation="MPSPHKRFAMIREFHLADWFTLGNAVCGVGALFSCMTYLETHDL
                     RHIYFACTLVLAALVFDVLDGRIARWRQKSSAMGRELDSLADVISFGVAPAIIAYACG
                     MQGLYDRVVLAFFVACGVSRLARFNITAEALAEGPSGKVKHFEGTPIPTSIVLVGLLA
                     LAASMGAVREELWLGKLMLGGFTLHPLVLLFALSGSLMISRIRIPKL"
     gene            complement(13549..14337)
                     /locus_tag="PK518_05450"
     CDS             complement(13549..14337)
                     /locus_tag="PK518_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRM48392.1"
                     /translation="MAFLTIERDGAILTVTMNQPETRNALTGNTAVAEFVQLCADVRK
                     DAGVKVVILTGAGPIFSSGGNVKDMKRFFDDALTPDLIREEYRQGIQQIPLALYELDV
                     PVICAVNGPAIGAGLDLTCMCDIRICSETATFAESFVRVGIVPGDGGAWLLPRAVGMA
                     KAAEMSFTGEAISAQEALACGLVSRVVPADQLLPTARALAEKIAANPGAVMRMTKRLL
                     REGQGATLATLLELSAGYQAIAHKTADHREAVTAFIEKRAPRFQ"
     gene            complement(14443..15480)
                     /locus_tag="PK518_05455"
     CDS             complement(14443..15480)
                     /locus_tag="PK518_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM48393.1"
                     /translation="MDNDLFADAARQLLGGECTPAVVRAIEAGGLGAPQAEALWAQIE
                     AAGLADALLDEAEGGAGLGLSQLFGVLEQAGAHALPLPLGDTLLARALLAQAGMARPA
                     GAIALASGERTQDGGLRCPLVRGGRVAASVLVQAGDGWHLLAVQAAQTTPQALALDAA
                     FAWSPSQMLAAPVVPVAQGLDARTLQAALLAPQMAGALMHVFQRTLQYANERQQFGRP
                     IGKFQAIQHQLAVMSEHVFAARMAAQLGCSGAGLAADRLPDRLRVATAKARCSEAALV
                     VSELAHAIHGAIGFTAEYDLQLYTRRLHAWRQAAGSESYWHDVAGSALLEHQGLTLDM
                     VRRITDVETAH"
     gene            complement(15492..16649)
                     /locus_tag="PK518_05460"
     CDS             complement(15492..16649)
                     /locus_tag="PK518_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM48394.1"
                     /translation="MSTLDSLALTRIPPADEALRAEVRAFLLDAIQDLPAHVRARSWG
                     GYSAELSRKLGAKGWIGVTLPKEYGGGGRSAFTRYVLVEEFLACGAPVGSHWIADRQS
                     APLILKYGTEAQKRFYIPRVCRGEAFFCIGMSEPGSGSDLASVKTRATPNEKGFVLNG
                     QKIWTTNAHHCQYMIALVRTSGSSEDRHKGLSQVIVDLSLPGVTVRPIEDLSGDSHFC
                     EVFFDNVQLSADALIGAEGQGWEQVTAELAFERSGPERLFSSIVLFDQWLDYLRTPQG
                     RSASSVRLAGKVLTQLSPLRAMSVAIQDKLVRGESPIVEAALVKELGTTLEQMIPAAI
                     ADDLFAREGEDVPVELLLTLKYVTEAAPSYSLRGGTRDILRGMIARGLGLR"
     gene            complement(16663..17649)
                     /locus_tag="PK518_05465"
     CDS             complement(16663..17649)
                     /locus_tag="PK518_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48395.1"
                     /translation="MPLHRRSTLRALVAAGLVALGAAMAPAAQAQAFPSKTIKFVVPF
                     GPGSGTDTSARYFAKKLQDLTGQAVVVENKPGANGFLAVKQVLTAPADGYTVFIGSNS
                     TLAVNAALFKELPYDPVKDFAPLTMMMRAPAMLVVPPGSPNGDLKSLIAYAKAHPGKA
                     NFGAGSAGYQLMGELLNDRAGLQTVHVPFKSAGETMTAVASGTVDFAFAEVTAVLELA
                     KGGRVKILAVAADQRVPNALDVPTMSEAGLPGFEAYTWVGAMVSAKTPTAETAKLAAL
                     FTTIAKMDETRAFYERLGATPMTGGPAQMHEFQKGEIALWKRIVQQAKVPLQ"
     gene            17751..18665
                     /locus_tag="PK518_05470"
     CDS             17751..18665
                     /locus_tag="PK518_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM48396.1"
                     /translation="MNLRQLHQFVTLAETGNFHRAAERLHMAQPPLSVSIRKLEDELG
                     APLFERTGTGVLLTPAGQAMLEDARRALQHAEQCRQAVQHARAGTGGRLRLGIIGSAT
                     YALLPRLIPSLRARYPQIALELTEATSSEILEGLLARRFDAGLLRFPVLDASGFDLTP
                     LDRDDFVLAVAESSPLAARGAIALHEAAREPFIMYPRAKVPGLAALAMMRCQASGFAP
                     RVAQEASQVQTIMSLVASGLGVGLVAGVARQAVPRGVVCLALTDNPPGFHISIALARL
                     AGDTSRVVQRFTEHALECGASTGAPALQ"
     gene            complement(18710..20791)
                     /locus_tag="PK518_05475"
     CDS             complement(18710..20791)
                     /locus_tag="PK518_05475"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRM48397.1"
                     /translation="METNNTPPYVPRMRLYQDWLRAERGLAFADYPALWRWSVTELDA
                     FWQSIWDYHALASPTPHRAVLARNRMPGAEWFPGAQVNYARQVLRHVAAAHGAGLAAV
                     ISEDEKGRVRALSWPELKRQSAALALHLQAQGVQPGDRVAAYLPNMPETMVAFLACAS
                     IGAVWSVCAPDMGTSAVLDRLRQIEPKVLIAVDGVTWAGVDMDRTPVLAELRAQLPSL
                     RHLLLVNNLDAAKTIADSARYTDVVARNDAEVAAFEPLWLPFDHPLWIVYSSGTTGLP
                     KPIVHSHGGIVLVAMVTGLHMDLGASYAANSLGERFCWYSSTGWVMWNCQVAGLLSGT
                     TCVIFDGSPGGPRQAPDWGTLWRFAARHRVTFLGSGAAFFAGCMKAGVDLARCGDLSR
                     IRALGSTGSPLSAEVQEWGSAQFAAIAATPAQEDIWWLNISGGTDFAGGFIAGTPELP
                     QRPGLMQCRMLGAAVEAWNEAGAPVTGQVGELVCTQPIPSMPLAFWNDPGNARYLASY
                     FEMYPPGHGRRPGGGDLGPEAGSVWRHGDWLRIGDEEGRGEWCVIYGRSDATINRHGL
                     RMGTSEIYSAVEALPEVLDSMVVDLEYLGRESFMPLFVVLRAGAVLDDALRGRINAAI
                     RQRLSPRFVPDAIEQVPEVPRTLSGKKQELPIKKLLLGQPLARVVNQEAMANPGCLAW
                     YVDYAERRSAS"
BASE COUNT         3471 a         7250 c         7034 g         3300 t
ORIGIN      
        1 atcggctacg tggccttcct cgacccaccc aaggactcca cggccgcggc cctgaaggcc
       61 ctggccgagc accaggtggc ggtgaaggtg ctgaccggcg acaacgagct agtggccgcc
      121 aagatctgtc gcgaggtggg cctggcgcag cagggcgtgc tgctggggcg cgacatcgag
      181 gccatgaacg accagcagct tggcgaggcc gtggagaacc acaacctgtt cgccaagctc
      241 acgccgctgc acaaggagcg catcgtgcac cggctcaagg ccaatggcca tgtggtgggc
      301 ttcatgggcg acggcatcaa cgacgcaccg gcgctgcgcg cggccgacat cggcatctcg
      361 gtggacagcg cggtggacat cgccaaggag gcggccgaca tcatcttgct ggagaaaagc
      421 ctgatggtgc tcgaacaagg cgtgcaggag gggcggcgca ccttcgccaa catgctcaaa
      481 tacatcaaga tcaccgccag ctccaacttc ggcaacgtgt tctcggtgct gatcgcggcc
      541 gcgctgctgc cttttttgcc gatgctgccc atgcacctgc tggtgctcaa cctgctgtac
      601 gacatctcgc agatcgccat accgttcgac aacgtggacg aagaaatgct gcgccagccg
      661 ctgcgctggc agcccggcga catcggccgt ttcatggttt tcttcgggcc gctgagctcg
      721 gtcttcgaca tgctcacgtt cgccatgatg tggtgggtgt tcggcgcgca gacgccggcg
      781 cagcagacgt tgttccagtc gggctggttc gtcgtcagcc tgctcacgca ggcactcatc
      841 gtgcacctga tccgcagccc caagctgccc ttcatcgaga gccgcgcctc gctgccgctg
      901 atggcgatga cgctcgccat cgtcaccgtg gggctgttcc tgcccatggg gccgctggcg
      961 cattacttca agctgcaggc cctgccgccg ctgtacttcg cgctgttgcc cctcaccatg
     1021 ctgggttata tggcgctgac ccaggccatg aagggttttt accgacgccg ctggggttgg
     1081 cagtaagctg cacttctcac cccctcgcac ggaggacaag cccatggatt ggaccaccca
     1141 ccaggtcttc aaccagttcc ccgaactggg cgactacgac ccgctggcca gcgacccggc
     1201 gctgtgcgag gcgctggcgc gcgccgatgc cggttgggcc aggccagcgc tggacagcta
     1261 tgcgcggcgg ctgggccggg ccgaaaccta caccctggcg caacaggcca accgccatgc
     1321 gcccgagctg caccgctttg acacgcgcgg tcggcgcatc gacgccatcg agttccaccc
     1381 ggcctggcac cagctgatgg cgctgtaccg cggccagggc ctggtgtcgc tcccttttcg
     1441 agagcagcag ccgggccgct ggactgcctg ggccgccggt ttttacctgc acggccaggt
     1501 ggagcaaggc acgctgtgcc cggccaccat gacccaggcg gccattccgc tgctgcagcg
     1561 cgaggacgcg ctgtgggcgc agctcaagga caagctgtac agcgacacct acgacgcgcg
     1621 cgatatgccg gctgcggaca aggccggcat ctggctgggc atgggcatga cggagaagca
     1681 gggcggctcg gacgtgcgcg ccaacaccac cgtggccacg gccatgggcg ccggggggcg
     1741 cggcggcgaa tacctgctgc gcggccacaa atggttcttc tccgcgccgc agtgcgacgc
     1801 gcacctggtg acggcgcgcg tcggtgcgga tggccccttc gcctgcttct acgtgccgcg
     1861 ctggcgcccc gacggcagcc gcaacgccgt gcgcgtgcag cgcctgaagg acaagctggg
     1921 caaccgcagc aacgccagtg ccgaggtcga atttgaggat gcctggggca tcctgatcgg
     1981 cgaggagggc cggggcattc caaccatcat cgagatggcc ggctatacgc gcctgaactg
     2041 cgtggtgggc agcgccgcca tcctgcgcca ggccacggtg caggccattg cctacgcgcg
     2101 ccggcgccag gccttcggca aggcgctggc ggatcagccg ctgatgcgcg ccgtgctgac
     2161 cgacctggcg ctggagagcg aggccgcgct gcagctggcc atgcgtctgg cccaggcctg
     2221 cgaggatgca accagctcgc cgggcgaacg cgcctggcgc cgcatcatga cgccggccgc
     2281 caagttctgg gtctgcaagc gcgccgtgga gctgaccggc gaagccatgg aggtgctggg
     2341 cggcaacggc tatgtcgagg acggcgtgct ggcccggctg taccgcgagg cgccggtgaa
     2401 ctccatctgg gagggctcgg gcaacgtcat gtgcctggat gtgctgcgcg ccctgcaacg
     2461 cgatggcgac gccgcccagg ccctgctggc cgagctgcag tcggcagccg cgggcgaacc
     2521 acgactggcc gtggccctgc gcgagctgct ggccctgctg gccctgccgc ccgagcagca
     2581 gcaagccctg ggccggctgc tggcccagcg cctggtgctg ctggcccagg cctgcctgct
     2641 gcgccggcat gcgcccagcg ccatggccga cgccttcatc gccacgcgcc tagccgatgg
     2701 cggcgcgggc cgcgtggccg gtgccatgga catgcgcggc ctggacgtcg ccgccatcct
     2761 ggcgcgggcc tttcccgcct gacaagaaca cttacagcaa aaacaggctt tagcgcaatc
     2821 gtataaagcg ccaatagctc acttattgat agtactcacc atgctcaccc tccaagaaat
     2881 acaggatgcc gccgcccgcc tgagcggcca ggtgctggac acgccctgcg tcgagtcgcg
     2941 cacgctgtcg cagatcgtcg gcgcgcaggt gttcctcaag ttcgagaacc tgcagttcac
     3001 ggcctcgttc aaggagcgcg gcgcctgcaa caagctggcc cagctcacag ccgaggagcg
     3061 cgcccatggc gtggtggcca tgagcgccgg caaccacgcc cagggcgtgg cctaccacgc
     3121 gcagcgcctg ggcctgcgcg cggtgatcgt catgccgcgc ttcacgcccg gcgtgaaggt
     3181 ggagcgcacg cgcggctttg gcgccgaggt ggtgctgcac ggcgacacgc tggaggaggc
     3241 gcgccagcat gcctaccagc tggccgagca gcagcagctg tgcttcgtcc acccctacga
     3301 cgacgaggcc atcgccgccg gccagggcac gatcgcgctg gagatgctgg ccgaacagcc
     3361 cgatctggac gcgctggtca tcgccgtcgg cggcggtggt ctgctggcgg gcattgccac
     3421 ggccgcgcgg gcgctgaaac ccggcctgga catcgtcggc gtgcaggcgg cgcgctttcc
     3481 gtccatggtg aatgccatcc agggtacgca gcacccgcag ggccagtcca cgatcgccga
     3541 gggcattgcc gtgggccagc ccggggagat gacgcgcgag gtcatctccc gcctggtgga
     3601 cgatctgctg ctggtgcagg agggcgacat cgagcaggcg gtgctgatgc tgctggagat
     3661 cgagaaaacc ctggtcgagg gcgcgggcgc cgccggcctg gccgcgctct tgcgctaccc
     3721 cgagcgcttt cgcggcaaga aggtggggct ggtgctctcg ggcggcaaca tagacccgct
     3781 gctgctggcg gccatcatcg agcgcggcat ggtgcgcagc gggcgcctgg cgcgcatcct
     3841 ggtcagcgcg cgcgacattc ccggcgtgct ggcgcgcatc accgccaccg tggccgaggc
     3901 cggcgccaac atcgaggagg tgcaccacca gcgcgccttc accatgctgg cggcgcagaa
     3961 cgtggagatc gagctggtgc tgcagacgcg cggcaaggcc catgtgcagc aggtgctgga
     4021 tcagctgcgc gccgcccata tggaagccga gctgcgctga gagcgctgat acgggttagg
     4081 cctcagaacc gcaaaccggc gcgcgactac actgaccaga accacgccac cagacacagg
     4141 agaccaggca tatgacccac cgccccatcg ccccgaatgc cgccgacgtg gaggtggcca
     4201 ccgccaccga ccccatcgag accgtagtgg acgtgattcc cttcgtgatc ccggccgcgg
     4261 gcgctttggt gatcttcctg ctggccttca ttgccgtgtc catgggctga ccggctgcgc
     4321 ccccactgac aaggccccgc aatgcggggc cttgttgttt ctccgacatt ctgtgcatat
     4381 tgtcatgcgc agtttgcata gttttacccc ctggcccaca gacaaattcc ggggtgcttc
     4441 atagaatcaa gggttccggt ctgactgcgc catgcccgcc cagccttggt gcctgtcgca
     4501 gccgcccggc ctactttcaa cccaagccgt tttttcaaag gcagcgccca tggtgcgcca
     4561 gcctgtgcca agcaagcccg gtgcgctctc tttgaaaaaa ctgtttttca acttgtagag
     4621 aagcccgcga tgaacgctcc caccctgcaa ggcctgacca ttcaggcccc cgcctacgtc
     4681 aagaacgcca agctgatcgc ctgggtcgcc gaaatggccg ccctgtgcaa gcccgccagg
     4741 atccactggt gcgacggctc gcaagaagag tacgaccagc tttgccagca gctggtcgat
     4801 gccggcacct tcaagaagct caaccctgcc aagcgccccg gcagctacct ggcctggtcc
     4861 gacccgtccg acgtggcgcg tgtggaagac cgcacctaca tctgctccga aaagaaggaa
     4921 gacgccggcc cgaccaacaa ctggatggcg cccaacgaga tgcgcgccac gctggcgccg
     4981 ctgttcgacg gctgcatggc cggccgcacc atgtacgtgg tgcccttctc catgggcccg
     5041 ctgggcagcc cgatcgccca tgtgggcgtg gagctgtccg acagcgccta cgtggccgtg
     5101 aacatgaaga tcatgacgcg catgggccgc gccgtgtacg acgtgatcgg cgccgatggc
     5161 gaattcgtgc cctgcatgca caccgtgggc gcgccgctgg ccgccggcca gaaggacgaa
     5221 accagctggc cctgcaacgc caagaccaag tacatcgtgc actaccccga gacgcgcgag
     5281 atctggtcct acggctcggg ctacggcggc aacgccctgt tgggcaagaa atgcctggcg
     5341 ctgcgcatcg cctccaccat ggggcgtgac cagggctggc tggccgagca catgctgatc
     5401 ctgggcgtga gcaaccccga gggcaagaag taccacgtcg ccgccgcctt ccccagcgcc
     5461 tgcggcaaga ccaacttctc catgctggtg ccgccccagg ccttcaacgg ctggaaggtc
     5521 accacgattg gcgacgacat cgcctggatc aagccccagg ccgacggcag cctgcgcgcc
     5581 atcaaccccg aggccggcta cttcggcgtg gcccccggca ccaactacca cacgaacccc
     5641 aactgcatgg ccagcctgga caaggacgtg atcttcacca acgtcgcgct gaccgatgac
     5701 ggcgacgtat ggtgggaggg catggagaag gacaccggcg tcatgcccgc gcacctgatc
     5761 gactggcagg gcaaggattg gacgcccgag gacggcaagg ccggccgcaa ggcagcacat
     5821 gccaacgccc gcttcaccgt ggccgccacc aacaacccgg cgctcgacga ggcctgggac
     5881 gaccctgccg gcgtgaagat cgacgccttc atcttcggcg gccgccgctc caccaccgtg
     5941 ccgctggtga ccgaggcacg caactggacc gaaggcgtgt acatggccgc caccatgggc
     6001 agcgagacca ccgccgccgc ctttggcgcc cagggcgtgg tgcgccgcga cccgttcgcc
     6061 atgctgccct tcatgggcta caacatgagc gactacttcc agcactggct gaacctgggc
     6121 gccaagatca gcgcccaggg cgcacagctg ccgcgcatct acaccaccaa ctggttccgc
     6181 aagaacgccg agggcaagtt cgtctggccc ggctatggag agaacatgcg cgtgctcaag
     6241 tggatgatcg atcgcatcga gggccaggcc caggggcagg aaaccgcctt cggcattgcc
     6301 cctcagtacg ccgagatcaa ctggggtgga ctggacttca gcgccgagca gttccagaac
     6361 gtgaccagca tcgacaaggc cgcctggatc gacgagctga agctgcatga cgagcatttc
     6421 gccatgctcg ctcagggcct gccgcgagag ctgttgaacg ccaaggccga tctggagcag
     6481 cgcctgagcg cctgaaactg tgcctgacca ccaaggcccc gcagcatgtc cgtgctgcgg
     6541 ggccttgctg tttgcgactg caagtacggg cgcaaaaaag ggcaccctga ggtgcccctg
     6601 gattgcggcc tgacccggta cgggccgggg cagtcagcga tagcggcgca tgtcctcgac
     6661 cgcgatgcca ttcatggcgc ggctcaggtc cgccaggatg cgcgcgtcgt gcagcgcggc
     6721 gttccaggtc ttttcatctt cctgggcgcg tcggtaggcc tcggtccagg cctcggtacc
     6781 cgagagcacc agcgacacca gtttccgcac cggtccggcc agcagggcca tgccggcgaa
     6841 ggcgataccc cacagcacca gccaggccgc cagcaggtgg ccgtcgctcc agctgtcgat
     6901 gagcgtattc gcggccacca tcaacgctgc cacggtcgcc gccagggcca gggatgtggc
     6961 accacgcgca ccgctggaac gggcggcggc ctggcgcaga tagcgcgcgg cgccccgcat
     7021 gcgggccagg ccacggtgtt gggtataaag ctcgatggat gcagatgtag tcatgatgaa
     7081 agcagaatgt cacggacggc gagcttgccg ggcgactgga gtaatactag ggttaaccct
     7141 gaattctgtc caatgcattg ttggaatcac ccttgccggt atgcgtaatg acccatcctg
     7201 cgggttcgcc atccaccaca caccatgaag ctgcaactgc tgtccgatct gcacctggag
     7261 attcaccccc atttccggcc cagcccggcc ccaggtgccg acctgctggt gctggccggc
     7321 gacataggct cctaccagcc aggctccctg ctggcggatg atgacttcgg tctggcgcgc
     7381 ttttcgccgt tgccgcagta cgccggctgg cccaccccgg tggtctttgt tccgggcaac
     7441 catgagtacg acggccagga ctttgatgcc gcccacgcgc gcctgcgcca cgtctgcgac
     7501 cggctggggc tgatctggct ggaacgcgaa agcctggtac tggacggcgt gcgcctggtg
     7561 gggaccacgc tgtggagcga ctttgacgcc ctggccgacc accatggcat caccgacctg
     7621 ggccagcgcc tgaagcggcg cgaaaaggcc tttcgcgccg ccgacttcta cctggccaag
     7681 accggtggca ccaggcgcgg cgagcccttt ctggcggcgc agctgcgcga gcagggcctg
     7741 gcctgccagg cctggttgcg ccaggcactg agccagccat ttgacggcac aacagtagtg
     7801 attacacact ttgcgcccag cctgcgcagt gccgacccac ggtatggctt gacgcccggc
     7861 acggccggtt tttgcaacgc catggacgag ctgctgcccc gggcccgcct ctggctgcac
     7921 ggccatctgc atgcacccag cgactacctg gcccagggcg tgcatgggga cggccgggcc
     7981 tggcattgcc gcgtggtggc caacccgctg ggctatgcgc gcaaggggga gcaggatgcc
     8041 ttcctgcccc agtgcgtgct caccgtatga ttgcccggcc tgcgccgagc gcgtgattca
     8101 ggtcaagatc aaaggcgccg ccgcggatta gtctgtcacc ctgtttacgg agaacctcgc
     8161 atgaacatcc tgcttgctgt cgatggcagc ccctacacca agaagatgct ggcctatctg
     8221 gccgcgcatg acgagatgct gggcacctcg cacagctaca cggccattac cgtgcaggcg
     8281 gcgctgccgc cgcgcgcccg cgccgccctg ggcaaggaga cggtggacag ctactacgcc
     8341 gaagagggcg agaaggtgct ggcgccggta gtgaagttcc tcgcccgcca cggcgtgcag
     8401 ctcaagaccg tgaccaaggt cggcccggcc ggcgagacca tcgccaaggt ggccgaatcc
     8461 ggcaagtacg acctcatcgt catgggcaca catggccatg gctcgctggg caagctggtg
     8521 atgggctcgg tcagcaccca ggtgctggcc aactgccagg tgccggtgct gctggtgcgc
     8581 tgatcagctc agaggctggg aggaaattgc ccgtgcccga ggggtgcgtc aaaacgcgcc
     8641 ccatttaggc gcaaaacgca gccatagctc gggctatggc gagtatttgc aacgacgatg
     8701 gggtgtgttc tggcgtgcca agcgggcatg ggggattgcc tctcacgctc tcagctcggc
     8761 agcgggctgc ggcaccaggc cgctcaggca ggtgtccatg gcccaggcca gaatctcggc
     8821 gttgccgtag ccacgctcgc gcagcatggc cagcatggga tcggccgagc gtgccagcag
     8881 ggcgcacaac accacatcgg gtggcagctc gcgccgcagc tgcccggccg cctgggcctg
     8941 cactatccag ccaagaatgc aggcgctcac ttcctgcatg gacgcgtggt acacatcgca
     9001 gccgcgcagc gcctgggcca gggccgagtt gcgctcgggc agcagcgggg tgtcgtcgcc
     9061 cagctgcagt tccagcagcc agtgcagcag ggcctgcagg cgctgcaggg ggctcagttc
     9121 ggcggacagt tcggccagga agcgctgcag ccgccccacg ccatgcacca tggcggcggc
     9181 gcacaaatca tccttgccgg caaaatgctt gtacaggctg gccttggcaa tgcccaccgt
     9241 ggcggccacc tcatcgacgg tcatggtctc gaagcccttg atgcccagca agcggctggt
     9301 ggcctcgacg atggcctcct cgcgtgcctg gtgcatctgc gccttgaacg agggcctggg
     9361 ctgcgacagc atgttcatgc ggcccatgtt agccagggcg cgataacccc aaaaaccctg
     9421 cttggcgacg gcccatcaac aggacggtct ttcagtgccg cgcttggtct ggttcggcca
     9481 ggaaatccag cagctccgcc tcgaaaccct cggggtcttc gagttgcggc cagtggccca
     9541 cgccgcgcag ctgcaccagg cgtgcatcgc gcagcacatg ggccagggcc tgcagcgcct
     9601 cgggaggggt gcagcgatcc tgctcgccac cgatgagcag cgtgggttga tgcaggcgca
     9661 tcaactcggg cgcgcggcgt gcaaagctgg ccagggcctg caacgcccgg cgataggtgg
     9721 cggcatggac acggcccatg gcgtggcgcg ccaggtgcag cccctcgggc agcgcggcgt
     9781 cgcctgccgc atgcaccacc agacgagcgg ccagctcgct catggacagg ccctcatcca
     9841 gtgcctccag gcgcggcgcc acccaggcgg cgatggcggc ggcgtcaaga ggcggcccac
     9901 ccgcgcacag caccaggcgc cgcacgcgca gcggggcgcg caaggccagc tccagcgcca
     9961 gcatggcgcc aaggccgtgg ccgaccagcg tcgcctcgtc gcactgcagg gcatccagca
    10021 gcagcaggca gcgttgggcc agacctttga agccgtaggg ctccaccggc gcgctgcggc
    10081 cgtagcccgg catgtcccag gccacggcgc ggtagccggc ggcggccagg gtttccagtt
    10141 gcggggcaaa gctcaggtga tcgccatcgg catcgtgcag cagcagcacc gtggggccgg
    10201 agcccagcag ggaaaaggtg ggtagcaacg acatggcttg tggctgggtg caggcgtggg
    10261 cgaacccgcc ggccgataat agcccccttg tcatccgctg ccgcgccccg agcgccgcat
    10321 gtctttcgtg aacagctccc tgccccgcac cgccgcccac ccgccccagt tttcaccacg
    10381 cgagtttcgt gacgcgctgg gcatgtttgc caccggcgtg gccatcgtca cggcgcgcag
    10441 cgagaatggc cagccgctgg gcatgaccat caactcgttc aactcggtgt cgctggagcc
    10501 gccactggtg ctgtggagcc tggcgcagcg ctcatcgctg ctgcaggcgc tgacgctcac
    10561 gcggcattgc gccatccatg tgctgacggc cgcgcagcgc ccgctggccg agcgctttgc
    10621 cacgcgcggc atcgaccgct gggccggcgt ggagcatcag ctgggcttgc agggtgtgcc
    10681 ggtgatcgcg ggcgcggcgg cagtgttcga atgccatgtg cgcagccagt acgccgaggg
    10741 cgaccacacc atcttcgttg gcgaggtgct gcgctgcggc caccgccagg gtctatcgcc
    10801 gctgctgtac cacggcggca tgttctatac cgagcacgcg ctgggccgcc tgtcacccaa
    10861 ggcgaccggc cccatggacc ggtgatgggc tggccgcagg cgcctacttg gccgcctcgg
    10921 gcagctcgat cttgacctcc agcacctcca ggtcatcctg gcgctcgaag tgcaccttca
    10981 gatcctcggg gttgatcttc acgtacttgg agatcaccgc caccagctcg cgctgcagcg
    11041 cgggcagata gtccggtttg ccggcgctgc ggccgctgcg ctcgtgggcc aggatgatct
    11101 gcaggcgctc cttggcgacc gcggcggtct tcttcttctc gccaagcaga aaggacagca
    11161 gggacatggc cgcttacctc ccaccaaaga tgcgcttgaa gaagccgggc ttgacggcct
    11221 cggtgaagcg caggggcttg tcctcgccca gaaagcgcgc caccacgtcc ttgtaggcct
    11281 cggacacatc ggtgcccccc aggtggatcg ccggcaggcc ctggttggag gcctgcagca
    11341 cgctttcgga ctcggggatc acgccgatca gcttgatgcg cagaatgtcc tggatgtcct
    11401 ccaggcccag catctgacca tcttccacgc ggttcgggtt gtagcgcgtg atcaggagat
    11461 gctccttgat cggctcgctg ccctcgatgg cgcgcttggt tttgctgctc agcatgccca
    11521 ggatgcggtc ggagtcgcgc accgaggaca cctccgggtt ggtcaccagc agcgcctcgt
    11581 cggcaaagtg catggccatc agcgcaccgc tctcgatgcc ggcgggcgag tcgcagacga
    11641 tgtagtcaaa gcccatctcg gccagatcct tgagcacctt gcccacgccg tcctgcgtca
    11701 gcgcttcctt gtcacgcgtc tggctggcgg cgagcacaaa caggttctcg cactgcttgt
    11761 ccttgatcag cgcctggttc aggttggcct cgccctggat aacgttgatc aggtcataca
    11821 ccacgcggcg ctcgcagccc atgatgagat ccaggttgcg caggccgacg tcgaagtcga
    11881 tgacggcggt cttgtggccg gcgagcgcca ggcccgatgc gaaggcggca ctggtggtgg
    11941 tcttgcccac tccccccttg ccggaggtca cgacgacgat tttggccatg ttctgatggg
    12001 tccttgcgtt gcttgtcttg cggatatgaa tctgaggaaa acggcttaca acggctcgat
    12061 gagcagcttt tcgccatcaa gccgcacctg ggccgggttg ccgcgcacgt cggctggcag
    12121 ctcggtctcg gtggtgcggt aaatgccggc cacggagacc agctgcggct ccaggcaggt
    12181 gctgaagatg cgcgcgctgg tgtcgccgcg cgcgccggca atggcgcggc cgcgcagcgg
    12241 ggcataaacg tgaatgttgc cgtcggcaat cacctcggcg ccaaagctga ccatggccag
    12301 caccaccagg tccgagccgc gcgcatacac ctgctggccc gagcgcagcg gcttgtccac
    12361 caccagcgcg ccgggcgccg gtgccggcac gggcacttcg accggcacct gcacctcgcg
    12421 caccacctca cggatgacct cgcgcggctg cgcacgcgcc gccggcatgt cgggcgcggc
    12481 caccaggccg gccgcatggg ccgcctgcat ctgcgcggga ttgccgccac gcacggccac
    12541 gggctgggtg cggtggctgc gcagcagctg caccagggcg gcgaagtcga tgggctgctc
    12601 ttcggcctgc acggccgaga ggtcgatcag caccgggtcg ttgtcgaaaa actcggggtc
    12661 gtcggcgctg cgggcggcca gctcggccgc aacggcggcg acgtccaccg tcttgagcac
    12721 catggccacc acggacagct gcgcgctctt gaggtctaga ggggtgcggg cctggcccgc
    12781 cgtgtcaacg gccatgaaac gatggggatc gaataaaggc gccacaggcg gcgcctcaaa
    12841 gccgcaaagt gtaccaatgc agccataacg gcctgcatca gcctgtgtca aagcttggga
    12901 atgcggatgc ggctgatcat cagcgagccc gacagcgcga acagcagcac cagcgggtgc
    12961 agcgtgaagc cacccagcat cagcttgccc aaccacagct cttcgcgcac cgcgcccatg
    13021 gaggcggcca gcgccagcag gccgacgagc acgatggagg tgggaatcgg cgtgccctcg
    13081 aagtgcttga ctttgcccga cggcccttcg gccagggcct cggccgtgat gttgaagcgc
    13141 gccaggcgcg agacgccgca ggccacgaag aaggccagca ccacgcggtc gtacagcccc
    13201 tgcatgccgc aggcgtaggc aatgatggcc ggcgccacgc caaacgagat cacatccgcc
    13261 agcgagtcga gctcgcgtcc catggccgag cttttctgcc gccagcgcgc gatgcggccg
    13321 tcgagcacgt cgaacaccag ggccgccagc accagcgtgc aggcgaagta gatgtggcgc
    13381 aggtcatgcg tctccagata ggtcatgcag gagaacagcg cccccacacc acacacggcg
    13441 ttgccgagcg tgaaccagtc cgcgaggtgg aactcgcgga tcattgcgaa gcgtttgtgc
    13501 ggagaaggca tggcgcgatt atccgcgccc gccctgcccg cgggcgcctt actgaaagcg
    13561 cggcgcgcgt ttctcgatga aggcggtgac cgcctcgcgg tggtccgccg tcttgtgggc
    13621 gatggcctgg tagccggccg acagctccag cagcgtggcc agcgtggcgc cctggccctc
    13681 gcgcagcaag cgcttggtca tgcgcatcac ggcgccgggg ttggcggcta ttttttcggc
    13741 cagggcgcgt gccgtgggca agagttgatc agcgggcacc acgcggctca ccaggccaca
    13801 ggccagcgcc tcctgggcgc tgatggcctc gccggtgaag ctcatctcgg cagccttggc
    13861 catgcccaca gcacgcggca gcaaccaggc accgccgtcg ccggggacga tgcccacgcg
    13921 cacgaagctc tcggcaaagg tcgccgtctc actgcagatg cggatgtcgc acatgcaggt
    13981 cagatccagc ccggccccga tcgccgggcc attcacggcg cagatcacgg gcacgtccag
    14041 ctcgtacagc gccaagggga tctgctgtat gccctggcgg tattcctcgc ggatcaggtc
    14101 gggcgtgagc gcgtcgtcga agaagcgctt catgtccttg acgttgccgc ccgagctgaa
    14161 gatggggccg gcaccggtga ggatgacgac cttgacgccg gcgtccttac gcacgtcggc
    14221 gcacagctgc acgaactcgg ccaccgccgt gttgccggtg agcgcgttgc gcgtctcggg
    14281 ctggttcatg gtgacggtga ggatggcgcc gtcgcgctcg atggtcagaa aggccatggg
    14341 aactccattt caaaaagagg agccgtcagc gcttgtttct cattggtttc aagcattttc
    14401 atgttgaaac tcaataacta caagcgcaag cagctatggt ttttaatgcg ctgtctctac
    14461 atcggtgatg cgccgcacca tgtccagcgt caggccctgg tgctccagca gggcgctgcc
    14521 ggccacgtcg tgccagtagg actcgctgcc ggccgcctgg cgccaggcgt gcaggcggcg
    14581 cgtgtagagc tgcaggtcgt attcggccgt gaagccgatg gcgccgtgga tggcatgcgc
    14641 cagctcggac accaccagcg ccgcctcgct gcagcgcgcc ttggccgtgg cgaccctcaa
    14701 acgatcaggc aggcggtcgg ccgcaaggcc cgcgccactg cagcccagct gcgccgccat
    14761 gcgcgcggcg aagacatgct cgctcatcac cgccagctgg tgctgtatgg cctggaactt
    14821 gccgatgggc cggccgaact gctggcgctc gttggcgtat tgcagcgtgc gctgaaagac
    14881 atgcatcagc gcgccggcca tctgcggcgc cagcagcgcg gcctgcagcg tgcgcgcatc
    14941 cagcccctgg gcgacgggta ccacgggcgc ggccagcatc tggctgggcg accaggcgaa
    15001 ggccgcatcc agcgccagcg cctggggcgt ggtctgggca gcttgaacgg ccagcagatg
    15061 ccagccatcg cccgcctgca ccagcacgct ggcggccacc cggccgccac gcaccagggg
    15121 gcagcgcagg ccaccgtcct gggtgcgctc accactggcc agcgcgatgg cgcccgcggg
    15181 ccgcgccatg ccggcctgcg ccagcagcgc acgcgccagc agggtgtcgc ccagtggcag
    15241 cggcagggca tgggcgccgg cctgctccag cacgccgaac agctgggaca ggcccaggcc
    15301 ggcgccgccc tctgcctcat ccagcagcgc atcggccagg cccgcggcct cgatctgggc
    15361 ccagagtgcc tcggcctgcg gcgcgcccag cccgccggcc tcgatggcgc gcaccacggc
    15421 cggggtgcat tcgcccccca gcagctggcg cgcggcgtcg gcaaacaaat cgttgtccat
    15481 gcccattcct ttcaacgcag tccgaggccg cgcgcgatca tgccgcgcag gatgtcgcgc
    15541 gtgccgccgc gcagcgagta cgagggcgcg gcctcggtca cgtatttcag cgtcagcagc
    15601 aattcgacgg gaacatcctc gccctcgcgc gcgaacaggt cgtcggcaat ggccgcagga
    15661 atcatctgct ccagcgtcgt gcccagctct ttcacaagcg ccgcctcgac gatggggctc
    15721 tcgccgcgca ccagcttgtc ctgtatcgcc accgacatgg cgcgcagggg gcttagttgc
    15781 gtcaacacct tgcccgccag gcgcacgctt gatgcgctgc ggccctgcgg cgtgcgcaag
    15841 taatccagcc actggtcgaa cagcacgatg gaggagaaca gccgctccgg gccgctacgc
    15901 tcaaacgcca gctcggccgt cacctgttcc cagccctggc cctcggcgcc gatcagcgcg
    15961 tcggcgctga gttgcacgtt gtcgaagaac acctcgcaga aatggctgtc gcccgacaga
    16021 tcctcgatgg ggcgcacggt gacgcccggc aaagacagat cgacgatgac ctgggacagc
    16081 cccttgtgcc tgtcctcgct gctgccggaa gtgcgcacca gcgcgatcat gtactggcag
    16141 tggtgggcgt tggtggtcca gatcttctgg ccattcagca caaagccttt ttcattgggc
    16201 gtggcgcgcg tcttaacgct ggccaggtcc gagcccgaac cgggttcgct catgccgatg
    16261 caaaagaatg cctcgccgcg gcacacgcgc gggatgtaga agcgcttttg cgcctcggtg
    16321 ccgtacttca ggatcagcgg cgcgctctgc cgatccgcga tccagtgcga gcccacgggc
    16381 gcgccgcagg ccagaaactc ctccaccagc acatagcgcg tgaaggcgct gcgcccgccg
    16441 ccgccgtact ccttcggcaa ggtcacgcct atccagccct tggcccccag cttgcggctc
    16501 agctcggcgc tgtagccgcc ccaggagcgg gcgcgcacat gggccggcaa gtcctggatg
    16561 gcgtccagca gaaaggcgcg cacctcggcg cgcagcgcct cgtctgcggg cggaatgcgc
    16621 gtgagcgcga gtgaatccag ggtgctcatg tccctgcctc ctttactgca gcggcacctt
    16681 ggcctgctgc acgatgcgct tccacagcgc gatctcgccc ttctggaact catgcatctg
    16741 cgccggcccg cccgtcatcg gtgtggcgcc caggcgctcg tagaaggcgc gcgtctcgtc
    16801 catcttggcg atggtggtga acagcgcggc cagcttcgcc gtctcggccg tcggggtctt
    16861 ggccgacacc atggcgccca cccaggtata ggcctcgaag cccggcaacc ccgcctcgct
    16921 catggtgggc acgtccaggg cattgggcac acgctggtcg gcggccacgg ccaggatctt
    16981 cacgcgcccg cccttggcca gctccagcac cgcggtgacc tcggcaaacg caaagtccac
    17041 cgtgcccgag gccacggcgg tcatggtctc gccggcgctc ttgaagggca catgcacggt
    17101 ctgcaggccg gcgcggtcgt tgagcagctc gcccatcagc tggtagccgg ccgagccggc
    17161 gccaaagttg gccttgcccg gatgcgcctt ggcatacgca atcaggctct tcaggtcgcc
    17221 attgggcgaa cccggcggca ctaccagcat ggcgggcgca cgcatcatca tggtcagcgg
    17281 cgcaaagtcc ttcaccgggt cgtagggcag ctccttgaac agcgccgcgt tcacggccag
    17341 cgtggagttg ctgcctatga acacggtgta gccatcggcc ggcgccgtga gcacctgctt
    17401 gacggccaga aagccattgg cgccgggctt gttctccacc accacggcct ggcccgtcag
    17461 gtcctgcagc tttttggcga agtagcgcgc cgaggtgtcg gtgccgctgc cgggcccgaa
    17521 gggaaccacg aacttgatgg tcttggacgg gaaggcctgc gcctgggcgg cgggcgccat
    17581 ggcggcgccc agggccacga ggccggcggc cacgagggcg cgcagcgtgc ttctgcggtg
    17641 caatggcatg ggtgatgtct cctggcaatg cgctttcggg acactttagg ccgctggtgc
    17701 catgctgtct aatattaaaa atattgatcg caatatccga tgaatatcaa atgaacctgc
    17761 gccaactgca ccagttcgtc acactggccg agacgggtaa ctttcaccgc gcggccgagc
    17821 ggctgcacat ggcgcagccg ccgctgtcgg tgtcgatccg caagctcgaa gacgagctgg
    17881 gcgccccact gttcgagcgc accggcaccg gcgtgctgct gacgcccgcc ggccaggcca
    17941 tgctggagga cgcacggcgc gcgctgcagc atgcggagca atgccgccag gcagtgcagc
    18001 acgcgcgcgc gggcaccggc gggcggctgc gcctgggcat catcggctcg gccacctatg
    18061 cgctgctgcc gcggctgatt ccgtcgctgc gcgcgcgcta cccgcagatc gcgctggagc
    18121 tgaccgaagc cacctcgtcc gagatcctgg aaggtctgct cgcgcgccgc ttcgacgccg
    18181 gcctgctgcg ctttcccgta ctggacgcaa gcggcttcga cctgacgccg ctggatcgcg
    18241 acgacttcgt gctggccgtg gccgagagca gcccgctggc cgcgcgcggg gcgattgccc
    18301 tgcacgaggc cgcgcgggag cccttcatca tgtacccgcg cgccaaggtg ccgggcctgg
    18361 cggccctggc catgatgcgc tgccaggcct cgggctttgc gccgcgcgtg gcgcaggagg
    18421 cctcgcaggt gcagaccatc atgagcctgg tggcctcggg cctgggcgtg ggcctggtgg
    18481 ccggcgtggc gcgccaggcg gtgccgcgcg gcgtggtctg cctggcgctg accgacaacc
    18541 cgccgggctt tcacatcagc attgcgctgg cgcggctggc gggtgacacg agccgcgtgg
    18601 tgcagcgctt taccgagcat gcgctggaat gcggtgcaag cacgggagcg ccggcgctcc
    18661 aatgaaaaat ggctgtagca caggtgcaac aagcgcatac agctattatt tatgaagcac
    18721 tccgccgctc cgcatagtcc acataccagg ccaggcagcc gggattggcc atggcctcct
    18781 ggttcaccac cctggccagg ggctggccca gcaacagctt cttgatgggc agctcctgct
    18841 tcttgccgct cagggtgcgc ggcacctcgg gcacctgttc tatggcatcg gggacgaagc
    18901 gcgggctcaa gcgttggcgg atggcggcgt tgatgcgccc gcgcagggcg tcatccagca
    18961 ccgcccccgc ccgcagcacc acgaacagcg gcatgaagct ctcgcggccc aggtattcca
    19021 gatcgacgac catggagtcc agcacctcgg gcagggcctc gacggcgctg tagatctcgc
    19081 tggtgcccat gcgcaggccg tggcggttga tggtggcgtc tgatcggcca tagatcacgc
    19141 accactcgcc ccggccctct tcgtcgccta tgcgcagcca gtcaccatgg cgccagacgc
    19201 tgccggcctc gggcccgagg tcgccgccgc cgggccggcg gccgtggccg ggcgggtaca
    19261 tctcgaagta gctggccagg tagcgtgcgt tgccggggtc gttccagaag gccagcggca
    19321 tggacggtat cggctgcgtg cagaccagct cgcccacctg gccggtaacg ggcgcgccgg
    19381 cctcgttcca ggcctcgacg gccgcgccca gcatgcggca ttgcatcagg ccagggcgct
    19441 gcggcagctc gggcgtgccg gcgatgaagc cgccggcaaa gtcggtgccg cccgagatgt
    19501 tgagccacca gatgtcctct tgcgccgggg tggcggcaat ggctgcgaac tgcgcgctgc
    19561 cccactcctg cacctcggcc gacagcggcg agccggtgct gcccagcgcg cgtatgcgcg
    19621 acaggtcgcc gcagcgggcc agatccacac ccgccttcat gcagccggca aagaaggccg
    19681 cgcccgaacc gaggaaggtg acgcggtggc gcgccgcaaa gcgccatagg gtgccccagt
    19741 cgggcgcctg gcgcggcccg ccggggctgc cgtcgaagat cacgcaggtg gtgcccgaca
    19801 gcaggcccgc cacctgacag ttccacatca cccagccggt ggagctgtac cagcaaaagc
    19861 gctcgcccag gctgttggcg gcgtagctgg cgcccaggtc catgtgcagc ccggtgacca
    19921 tggcgacgag cacgatgccg ccatgcgaat gcacgatggg ctttggcaac cccgtggtgc
    19981 cgctggaata gacgatccac agcgggtggt cgaatggcag ccatagcggc tcgaaggccg
    20041 ccacttcggc atcatttcgg gccacaacgt ccgtgtagcg ggcgctgtca gctatggttt
    20101 ttgcggcatc caggttgttt accagcagca ggtggcgcag gctgggcagc tgcgcccgca
    20161 gctcggccag caccggggtg cgatccatgt ccacgccggc ccaggtcacg ccgtccacgg
    20221 cgatcagcac cttgggctct atctggcgca ggcggtcgag cacggcgctg gtgcccatgt
    20281 cgggcgcgca cacgctccac accgcgccta tgctcgcgca ggccagaaag gccaccatgg
    20341 tttcgggcat gttgggcagg taggccgcca cgcggtcgcc cggctgcacg ccctgggcct
    20401 gcaggtgcag ggccagcgcc gcgctctgcc gcttcagctc gggccaggac agcgcacgca
    20461 cgcggccctt ctcgtcttcg ctgatcaccg ccgccaggcc cgcgccgtgc gccgcggcca
    20521 catggcgcag cacctgccgg gcgtagttga cctgcgcgcc cggaaaccac tcggcccccg
    20581 gcatccggtt gcgcgccagc accgcgcggt gcggcgtggg cgaggccagc gcgtggtagt
    20641 cccagatgct ttgccagaag gcgtccagct cggtcaccga ccagcgccac agcgccgggt
    20701 agtcggcaaa cgccaggccg cgctcggcgc gcagccagtc ctggtacagg cgcatgcgcg
    20761 gcacataggg tggggtgttg tttgtctcca tcgcgccagc gtagcagcgc ggcccgaggg
    20821 gcgtcagcct ccatcggtcg cgcgccggac agttgccgcc gcgcaaaaat tccgtttcca
    20881 aatcatgcgt gtctaggcgc gaagccgcag gcagtgctgt cgcacgacaa ggcgaagcaa
    20941 caacgacacg ggtgatttgg aaacggaata accctgtgcg gggccggccg taccatatgc
    21001 cccatgcaac acagcgacgt tctcatcgtt ggcgcaggcc cggccggcct gtcgc
//
LOCUS       DAOMTQ010000036        20971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_299_length_20971_cov_27.160212, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000036 DAOMTQ010000000
VERSION     DAOMTQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 20971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20971
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_299_length_20971_cov_27.160212"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK518_05480"
     CDS             complement(<1..724)
                     /locus_tag="PK518_05480"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HRM48398.1"
                     /translation="MSLEAFSHSEPLTLGVELELQLVSTHDYDLAPYAEDMLRLMHKT
                     PLPGSVVPEMTNSMIEISTGVCHSSSEVLGQLTQIRDALVKSADKLNIAVVGGGTHPF
                     QQWHERRIYDKPRFQELSTLYGYLSKQFTIFGQHVHIGCPDADAALLMLHRMSRYIPH
                     CIALSASSPYVQGQDTAFDSARLNSVFAFPLSGRAPFVLTWDEFGQYFEKMTHTGVVK
                     SMKDFYWDIRPKPEYGTIEIRVF"
     gene            complement(714..1976)
                     /locus_tag="PK518_05485"
     CDS             complement(714..1976)
                     /locus_tag="PK518_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRM48399.1"
                     /translation="MNELTDFWAQWLRPSAGLPTVQWALLLAVATVAGYLVQRHSGLP
                     KVVGYTLVGTIAGLAGFGGAVWPLQGIGLFLVELAVAVVLFEAGGRIPLRWFRHNPMV
                     LVQSVAESALTYAAVYWVMLWLGVPAPAAAPLAMVALAASPAVLTRVVNDTRAAGPVT
                     DRAVVLATLSSLYALALGSAQAGLLQRPASSLLGQIYPVLVVLGVSVAVGALLSLVMR
                     LALRVMSPTSENTTILLLALIAASTAVTAHMGGSAPLAALLGGMLLKQLHPRPWAWPR
                     QMGTATSLLTMLMFVLVSTVAAQGQWSAPVAGSVLALIVVRLAAKAVGVGLGNVGSGA
                     SWRQALWVGCAMTPMSAIALLVASQFVQASADTGELIARIALPAILVMELLGAVIAAF
                     AIYRAGESSKPWLTPGGRNQRGGSGRES"
     gene            complement(2129..4117)
                     /locus_tag="PK518_05490"
     CDS             complement(2129..4117)
                     /locus_tag="PK518_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HRM48400.1"
                     /translation="MSRKRADLAQLLAALDPEAAAAQRHLQLIALLDWVRGDSQVVQA
                     ATDRVAQLVQAAEQSADTRTRLQAWWAALTGQLDITTLLADFGFAQRSSMASELAERL
                     RHKLLPTSPDTLDASELFMLALPHEFDARWLAALDAALLERLLAVLIPAPEDSSAGAS
                     AWQRDLLDAISYCAGQILATGFAPELRLRMSEATRQAQPFHVLLRDTESLRVEVLHPL
                     RTDDRLQEAVQRLREHLDACRAAAGSVYAHFEDEGISVGLVFRLRQLRERILRVRELL
                     ECLLAPRPVVAATRFMARLVNVGRERRSLRALIAVNSSLLAAKVAERSAETGEHYITR
                     TGGEYLAMVRQAAGGGLVMAATTLVKFGLYALALSAFWGGFMAGVNYAISFVLIQLLH
                     FTVATKQPAMTAPAMAAKLKELDDDADVQAFVDKVTQLVRSQVAAVLGNVLLVFPAAL
                     ALAWLIAQALGRPAIDAGHAMQTLDTLHLLGPSLLFAAFTGVLLFASSIFAGWVENWF
                     VLHRLDSAIQYNPRITGVLGRRRAARWAHFLRGNISGLAANVSLGFMLGLTPAFAAFF
                     GLGLEVRHVTLTTGQIGVAAATLGMDAVHSQAFWWAVALIPFNGALNVIVSFYLAFHL
                     ALRAHNVARVDRRRIYRALRARWRHAPLSFFAPTRGSV"
     gene            complement(4114..4521)
                     /gene="apaG"
                     /locus_tag="PK518_05495"
     CDS             complement(4114..4521)
                     /gene="apaG"
                     /locus_tag="PK518_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRM48401.1"
                     /translation="MPKYQFTVEVQPQYLPEQSSPDEDAYGFAYTITISNTGDTAAQL
                     IARHWIICDANGHRQEVKGLGVVGQQPLLKPGEAFEYTSGCRLRTASGSMHGSYLCVA
                     EDGEQFDCPIALFLLEADSGGDPGRPATPRVLH"
     gene            4561..5241
                     /gene="rpe"
                     /locus_tag="PK518_05500"
     CDS             4561..5241
                     /gene="rpe"
                     /locus_tag="PK518_05500"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM48402.1"
                     /translation="MSRTYRIAPSILSADFARLGEEVRNVVAAGADWIHFDVMDNHYV
                     PNLTFGPMVCQALKPHAVTPDGRPVPIDVHLMVQPVDALAEAFAQAGADLISFHPDAS
                     AHVHRSIQAIKAQGCQAGLTFNPAMSLDVLDWVIEDIDLILLMSVNPGFGGQSFIDST
                     LRKVEAARRRIEASGKDIRLEVDGGIKADNIRRVADAGADTFVAGSAIFGKPDYKAVI
                     DAMRRALA"
     gene            5238..5951
                     /gene="gph"
                     /locus_tag="PK518_05505"
     CDS             5238..5951
                     /gene="gph"
                     /locus_tag="PK518_05505"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466390.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HRM48403.1"
                     /translation="MTAPVLQGLDAAIIDLDGTMVDTLGDFAQALGAMLDDLGLPAIE
                     PAYIAQMVGKGSEHLLRSVLNHALTQAGQAPDASKIEAFYPNAWERYQQHYLAINGRH
                     SSVYPGVRQGLMALRGAGLRLACLTNKPTSFALPLLRAKGLDGFFEHVFGGDAFERKK
                     PDPLPVLKTCAALGTLPARTLMVGDSSNDAAAARAAGCPVALVTYGYNHGQPVRAVDA
                     DCYVDALHELMPKWVCGLR"
     gene            complement(6064..7806)
                     /locus_tag="PK518_05510"
     CDS             complement(6064..7806)
                     /locus_tag="PK518_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM48404.1"
                     /translation="MRLNDIKVGPRLALSFGLVLLITAVIAAVGVWRLQDLAAATQRL
                     TSTDNERLRAAVQWRQGIDQNWIRTRAALLNADSSHLAQWQAEMDATSKGVDVSRKTV
                     ERLLETDEGRRQIADIDKAREAYRKPRADLFKRKAAGEDVGPLVDSQLKPLSDAYIAT
                     LRTLEERQLMLYERTRDQATAEAAQGRMILIAAAALALLLGAGLAWLLSRSITAPLQL
                     AARRAGQIAEGDLTQPIAAQGRDEAANLLHALGEMQASLTRVVAGVRGNAESVATASA
                     QIAQGNHDLSARTESQASALEQTAASMEQLGATVRQNADNAAQANQLAMNASTVAVQG
                     GAVVAEVVDTMRGINDASRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAV
                     VAGEVRSLAQRSAEAAKEIKQLITDSVGRVEQGTQLVDKAGNTMTEVVNAIRRVTDIM
                     GEISAASREQSSGVAQVGEAITQMDQATQQNAALVEESSAAASSLNQQAGDLVQAVAV
                     FKLAGHAGITTGKASAASQARPAPAPRVAPRPATAPKPVARAASKPAAVLSAPSKASP
                     AAVAAGGDDDWDSF"
     gene            8161..>8558
                     /locus_tag="PK518_05515"
     CDS             8161..>8558
                     /locus_tag="PK518_05515"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655421.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRM48405.1"
                     /translation="MITELEFKSLALEGYNRIPLMAEAFADLETPLSLYLKLAHGKGD
                     GRHSFLLESVVGGERFGRYSFIGLPARTLLRASGFGAAARTEVVTDGQVVESAGGNPL
                     DFIAAYQKRFKVALRPGLPRFCGGLAGYFG"
     assembly_gap    8559..8658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8659..9497
                     /locus_tag="PK518_05520"
     CDS             <8659..9497
                     /locus_tag="PK518_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRM48406.1"
                     /translation="RSFAKADYLAAVEQAKELIAAGDFMQVQVGQRIQKRYTESPLSL
                     YRALRSLNPSPYMYYYHFGDFQVVGASPEILVRQEAVEEGTKVTIRPLAGTRPRGATP
                     EKDKAAELELMGDPKERAEHVMLIDLARNDIGRIAKTGTVKVTEAFCIERYSHVMHIV
                     SNVEGILNEGMTSMDVLKATFPAGTLTGAPKVHAMELIDRLEPTKRGLYGGACGYLSY
                     AGDMDVAIAIRTGIVKDGMLYVQAAAGVVADSVPELEWKETEHKARALLRAAELVEEG
                     LE"
     gene            9494..9820
                     /locus_tag="PK518_05525"
     CDS             9494..9820
                     /locus_tag="PK518_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRM48407.1"
                     /translation="MTRAIDHVQHCTTMEDVRRFIDALDDVLVPLLVTRGGYMTQAAR
                     IKQCNTQVRDEDRIEAIVARVRARAATEGGQPEVMEAIYRSMMEAYIAYEHREFARMR
                     APGDES"
     gene            9817..10389
                     /locus_tag="PK518_05530"
     CDS             9817..10389
                     /locus_tag="PK518_05530"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRM48408.1"
                     /translation="MKLLMVDNYDSFTYNIVQYFGELGAEVEVFRNDEITLEGIAARA
                     PDRLVISPGPCSPLEAGISVAAIQHFAGRLPILGVCLGHQSIGAAFGGKIIRAQELMH
                     GKTSVITTDEQGVFAGLPRQFTVNRYHSLAIERASCPEVLAVTAWTEDGEIMGVRHKE
                     LPIEGVQFHPESILTEHGHAMLKNFLEQRA"
     gene            10386..11045
                     /locus_tag="PK518_05535"
     CDS             10386..11045
                     /locus_tag="PK518_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRM48409.1"
                     /translation="MIDAQQLLLFIAAGWLLNLTPGPDVLYIVTRALKNGLRAGLVAG
                     LGIAAGCLVHVGAAAVGVGALLAASSTAFTLLKWLGAAYLLWLGLRLLRARGDGALAG
                     VVRETAGHAAVPVALKEVFLGGFWTNVLNPKVAIFFLAFVPQFIAPGAGNKALAFVLL
                     GVLFTVNAIPVNALWALAAAWLVRRVGAVQRGMHWLDRVAGLLFIGFGVRLALTDAPA
                     V"
     gene            11066..12097
                     /gene="trpD"
                     /locus_tag="PK518_05540"
     CDS             11066..12097
                     /gene="trpD"
                     /locus_tag="PK518_05540"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRM48410.1"
                     /translation="MSITPQEALQRTIEHREIFHDEMLHLMRMIMSGELSPVMTAAIV
                     TGLRVKKETIGEITAAAQVMREFSRKVSVADTTHLVDIVGTGGDGAGTFNISTCATFV
                     IAAAGAKVSKHGGRSVSSTSGSADAMEALGVNIQLTPAQIAQCIQEVGIGFMFAPNHH
                     PAMKNVAPVRKELGVRTIFNILGPLTNPASAPNILMGVFHEDLVGIQVRALQRLGAQH
                     ALVVYGRDGLDEISLGAGTLVGELKDGVVREYEIHPEDFGLRMAGTRALRVENPDQSK
                     AMLLAVLQGEEGPARDIVCLNAGAALYAANVAPTMAEGLQRAQQALASGAALAKLHEL
                     VAYTQKLAA"
     gene            12107..12244
                     /locus_tag="PK518_05545"
     CDS             12107..12244
                     /locus_tag="PK518_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48411.1"
                     /translation="MSLLDAPIWNDAGTWIVLGVSLLFIVVGLVMHQVIRRVLRKPPE
                     H"
     gene            12267..13067
                     /gene="trpC"
                     /locus_tag="PK518_05550"
     CDS             12267..13067
                     /gene="trpC"
                     /locus_tag="PK518_05550"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRM48412.1"
                     /translation="MSDILKKIVDVKFEEVAAAKRRMPLADMRRDAESRVLTRDFVGA
                     LRAKITAGQAAVIAEIKKASPSKGVIRADFIPADIAQSYAEGDGKVSAACLSVLTDRQ
                     FFQGQPDYLKQARASCQLPVLRKDFMVDPYQIYESRAMGADCVLLIAACLDDGLMAEM
                     EAIARSLDMAVLVEVHDAPELQRALKLKTPLVGINNRDLRSFEVSLQTTLALQKQVPA
                     DRLLVTESGILAPADVRLMRDAGVHAFLVGEAFMRASDPGLALARLFA"
     gene            13070..15364
                     /locus_tag="PK518_05555"
     CDS             13070..15364
                     /locus_tag="PK518_05555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM48413.1"
                     /translation="MLRLLRPPADPQRREWWWLAWVALLAAGLLVAGLWLEHQRVAAR
                     ERVLLAKQAGVMHDNLGMQLEAINNAMRHLQADQRKGLDGADGHARLMERMHNFADAM
                     PAVRTFSLLDADGRVLASSAADLVGRDLSGREYFQTAKQIGQTDALLVGRPFFGMLGG
                     WVLVLGRTVPGADGAFGGLLVAALDVEHFQTLLQSVRYAPDMRVSLSHGDGMRFMVLG
                     ADNEPDGVNLAQAGTLFTRHRESRQAASVLQGFPQPGYPPYVMALHKVQPSNLRMDKP
                     LVVAAGRPLDDVFAGWRDQAWRFALVYLLAFSAAGVALRLMHRRQNEARDQAQLLEAG
                     QRRQQEMLRRLVESLPGMLYQYQLEPDGRSHFPYASPGVTDIYGFAPEQLLADAAPVF
                     ARIHPEDLPKGQVNIQESARTLADWKSEYRVILPGIGERWLSGQARPQRLDGGAVLWH
                     GYIHDVTDMKRQALQLQETERLLQQLINDMPVGLCMVDAQRRIYFRNRRFLEHFGYAE
                     AEVPTLHEWALLAYPDGDYRQQAAHAWGQAMAGARAQGGVIAAQPYRVTARDGAQHVM
                     DIGGLLFGEHFLATFQDRTEQQAQSELLHRLAYVDGLTGIANRRQFDQQLEAEWRRCR
                     RSGKPLAVLLLDIDYFKQYNDLYGHQQGDECLKAVATALRGQLGRSHDLVARYGGEEF
                     VCLLPECDAAGALHKGQELCHAVQALGLAHAQSRVADVVTISVGVACQVPDGEGSPAA
                     LLQQADMQLYQAKAQGRNRALGAH"
     gene            15494..17791
                     /locus_tag="PK518_05560"
     CDS             15494..17791
                     /locus_tag="PK518_05560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM48414.1"
                     /translation="MQRFRREWWWLGLGALLVGAMLALGLWHERQQTEARERQHLAQQ
                     ARMLHDNLVPQLGAINHVLASLQADVPRWRQRPGGMAELNQRLQLFFAALPGVRSLNV
                     LDGQGRVLASSHDELLGQDFGRRDYFQMARTGMGPETLLVSRPFQGALGGWVLVLGRT
                     LTAADGSFDGLLTASLNPEYLHVVLQSVRYASDLFTSLTHGDGQRLMALGDANERAGD
                     NLAMPGSLFMQHLQSQRSDNLLRGPLRPGGADYLVAMRNLQPPGLHMGKPLVVGVGRT
                     TQAMWAQWRQRARFLALVYLLSCAALAGGLAALQRRQWRLWHTQELLERQQALQRQQE
                     DMLRRLADNLPGLLYQYQLEPDGRSHFPYASPGVTDVFGHPPESLRADAGPALARIHP
                     ADRVAGEAAMQESARTLGVWQGEYRVTLPGRGERWLSGQARPQRLRSGAVLWHGYVHD
                     ITEAKRQAMELQEKERLLRHLMEQMPIGLCIVDESGHFSFRNQRFAEYFGYPASEPLT
                     LQRWWQEVYPDADYRARVLALWNEAMDHADAHDGTLPGHEYRVTTRYGTQRIMAIGGI
                     AMGGRFMASFIDLTEQKAHSDLLRHLAFVDGVTGIANRRRFDEALQREWRDCLRKGKP
                     LALLMFDIDHFKAYNDFYGHQRGDGCIKAVAAVLRAGLGRPHDLAARYGGEEFVCLLP
                     DCGPQDARAKAQELCQAVRSLGLAHSASPVAAVVTLSAGVACLVPDERNSPEALLARA
                     DANLYRAKSAGRDRVDQGVETLQLQ"
     gene            17843..18553
                     /locus_tag="PK518_05565"
     CDS             17843..18553
                     /locus_tag="PK518_05565"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRM48415.1"
                     /translation="MHAEAATQLRSANPDDWPVAPGWQALTAGFFASAEGRSLLDFLR
                     QRLQAGAVLFPPEPLRALALTPPEAVRVLILGQDPYHGRGQAEGLAFSVAPGVRLPPS
                     LRNIFKEMQRDLGQPFPPLPDPGGSLVSWARNGVLLLNTCLTVEEGQAASHSGRGWER
                     LTDAVIRHVAEGPRPVVFMLWGGHAQGKRALIPADRGHLVLTSNHPSPLSALRPPAPF
                     IGNGHFGQARAFRERQGA"
     gene            18568..20172
                     /locus_tag="PK518_05570"
     CDS             18568..20172
                     /locus_tag="PK518_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRM48416.1"
                     /translation="MHNAQTLDWTLPYASQRSAVLGRNVVATSQPLAAQAGLAMLQAG
                     GNAVDAAIAAAMALTVVEPAGCGIGSDGFAICWDGRQLHGLNASGRSPAAWTPEYFAR
                     LGGIPEKGWNAVTVPGAVSAWVALSKRLGRLPFARLAQPAIDYARHGIPVSPTIARLW
                     ELGAAKLGDQPGFAECFLPAGRPLRAGEIFRSEAHARTLELIAATEGEAFYRGELAEK
                     MAAHAQAHGGAMTVEDLAAHQPDWVGTVSQRFGDSVIHEIPPNGQGIAALMALGMLDE
                     HGVGAHPVDDVRTVHASIEAMKLAFADLHQYNADIDYMRVAPSELLNPAYLKGRAALI
                     DPARAGAPSYGAPRPGGTVYLAAADASGMMVSFIQSNYMGFGSGVVVPGTGISLQNRG
                     HGFNLVPGHANQVGPGKRPSHTIIPAFAMHADGRPQMAFGVMGGPMQSQGHVQMALRV
                     LRYGQNPQAAADAPRWRVTGGKGVAVEPHFDAAVVAGLRALGHEVTVEAGNGVFAFGG
                     AQLVLRQGDVYIAGSDSRKDGCAVAF"
     gene            complement(20507..>20971)
                     /locus_tag="PK518_05575"
     CDS             complement(20507..>20971)
                     /locus_tag="PK518_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48417.1"
                     /translation="ATSTEAPAAGAMPELITAISAAEAAGLRDDCPAGTLLYTQIYDE
                     ATRERAEQLRQRLQQAGAGALRIPRIENVARTAAMRQQRPPVPWQQPTFVVHQARLRP
                     CAQALAQLVQPRWSPASRQKVWVTGLPTGLKGQPGTLELWLPAPEPERTGAR"
BASE COUNT         3183 a         7125 c         7276 g         3287 t
ORIGIN      
        1 cgaagacgcg gatctcaatc gtgccgtact cgggcttggg gcggatgtcc cagtagaagt
       61 ccttcatgct cttgaccacg ccggtgtgcg tcatcttctc gaagtactgg ccgaactcgt
      121 cccaggtcag cacaaagggc gcgcgccccg acagcggaaa ggcaaacacc gagttcaggc
      181 gcgccgagtc aaacgccgtg tcctgcccct gcacataggg gctggaggcc gacagcgcga
      241 tgcagtgcgg gatgtagcgg ctcatgcggt gcagcatcaa gagggccgcg tccgcgtccg
      301 ggcagcctat gtgcacatgc tggccaaaga tggtgaactg cttggacagg tagccgtaga
      361 gcgttgagag ctcctgaaaa cgtggcttgt cgtagatgcg ccgctcgtgc cactgctgga
      421 aggggtgggt gccgccgccg accacggcga tgttcagctt gtcggcgctc ttcaccagcg
      481 cgtcgcggat ctgcgtcagc tggcccagca cctcggagct gctgtggcac acgccggtgg
      541 agatctcgat catgctgttg gtcatctccg gcaccacgct gccgggcagc ggcgtcttgt
      601 gcatcaggcg cagcatgtcc tcggcatagg gcgccaggtc gtagtcgtgg gtgctgacga
      661 gttgcagctc cagctccacg cccagggtca gcggttcgga gtgggaaaag gcttcaagac
      721 tcacggccgg atcctccacg ttggttgcgg ccgccgggcg tcagccaggg cttggagctt
      781 tcgcccgcgc ggtagatggc gaacgcggcg atcaccgcgc ccagcagctc catgaccagg
      841 atggccggca gggcgatgcg cgcgatcagc tcgccggtgt cggcgctggc ctgcacaaac
      901 tgcgaggcca cgagcagcgc gatggccgac atcggcgtca tggcgcagcc cacccacagt
      961 gcctggcgcc agctggcgcc gctgcccaca ttgcccaggc ccacgcccac cgccttggct
     1021 gccaggcgca ccacgatcag cgccagcacg ctgccggcca ccggcgcgct ccactgcccc
     1081 tgcgcggcca cggtggagac gagcacgaac atcagcatgg tcagcagcga cgtggccgtg
     1141 cccatctgcc gcggccaggc ccaggggcgc gggtgcagct gcttgagcag catgccgccc
     1201 agcagcgccg ccagcggcgc cgagccgccc atgtgcgcgg tgacggcggt gctggcggct
     1261 atcagcgcca gcagcaggat agtggtgttt tcgctcgtcg ggctcatcac gcgcagcgcc
     1321 aggcgcatca ccagcgacag cagcgcaccc accgccaccg acacgcccag caccaccagc
     1381 accgggtaga tctggcccag caggctgctg gccggccgct gcagcaggcc ggcctgggcg
     1441 ctgcccaggg ccagcgcgta cagcgaggac agcgtggcca gcaccacggc gcggtccgtc
     1501 accgggccgg cggcgcgcgt gtcgttgacc acgcgcgtga gcaccgccgg cgacgccgcc
     1561 agcgccacca tggccagcgg agcggcggca ggcgcgggca cgcccagcca cagcatgacc
     1621 caatacaccg cggcataggt cagggccgac tcggccacgc tttgcaccag caccatgggg
     1681 ttgtggcgga accagcgcag cgggatgcgc ccgccggcct cgaacagcac cacggccacg
     1741 gccagctcca ccaggaacag gcctatgccc tgcagcggcc agaccgcgcc gccaaagccg
     1801 gccaggccgg caatcgtgcc caccagcgtg tagcccacca ccttgggcag gccgctgtgg
     1861 cgctgcacca gatagccggc caccgtggcc acggccagca agagcgccca ctgcacggtg
     1921 ggcagcccgg ccgatgggcg cagccactgt gcccaaaagt ctgtcagttc attcatgctt
     1981 gtccttccgt ctcgcgcggg cgtggcccgc ctgatgccgg agcagggggg agtcggctgc
     2041 actcccagcg ccttgtgtgc ccggaacgga tgcagccgct cggcggtctg catgctgcgc
     2101 gccatgcggc gcgcctggtt ccaccttatc agacgctgcc gcgcgtaggg gcaaagaagc
     2161 tcagcggtgc atggcgccag cgtgcgcgca gcgcgcggta gatgcgcctg cggtccacgc
     2221 gcgccacgtt gtgcgcgcgc agcgccagat ggaacgccag gtaaaagctc acgatgacgt
     2281 tgagcgcgcc gttgaacggt atcagcgcca cggcccacca gaaggcctgg ctgtgcaccg
     2341 cgtccatgcc cagcgtggcg gcggccacgc cgatctggcc cgtggtcagc gtgacgtggc
     2401 gcacctccag ccccaggccg aagaaggcgg caaacgccgg cgtcagcccg agcatgaagc
     2461 ccagcgagac attcgccgcc aggcccgaga tgttgccgcg caggaaatgc gcccagcgcg
     2521 cggcgcggcg ccggcccagc acgccggtga tgcgcgggtt gtactggatg gccgaatcca
     2581 ggcggtgcag cacgaaccag ttttccaccc agccggcaaa gatgctggag gcaaacagca
     2641 gcacgccggt aaaggcggca aacagcagcg atggcccgag caggtgcagc gtatccagcg
     2701 tttgcatggc gtgcccggca tcgatggcgg gccggcccag cgcctgggcg atgagccagg
     2761 ccagcgccag cgccgccgga aacaccagca gcacgttgcc cagcaccgcc gccacctgcg
     2821 agcgcaccag ttgcgtgacc ttgtccacga aggcctgcac gtccgcgtcg tcgtccagct
     2881 ccttgagctt ggcggccatg gccggcgccg tcatcgccgg ctgcttggtg gccacggtga
     2941 aatgcagcag ctgtatcagc acgaagctga tcgcgtagtt gaccccggcc atgaagccgc
     3001 cccagaaggc cgacagcgcc agcgcgtaca ggccgaactt gaccagcgtg gtggcggcca
     3061 tcaccaggcc gccgcccgcg gcctggcgca ccatggccag gtattcgccg ccggtgcgcg
     3121 tgatgtagtg ttcgcccgtc tcggcgctgc gctcggccac cttggccgcc agcagcgagg
     3181 agttgacggc gatcagcgcg cgcaggctgc gccgctcgcg ccccacgttc accagccgcg
     3241 ccatgaacct ggtggcggcc accaccgggc gcggcgcgag caggcattcc agcagctcgc
     3301 gcacccgcag gatgcgctcg cgcagctggc gcaggcgaaa caccaggccc accgagatgc
     3361 cctcgtcctc gaaatgcgca tacacgctgc cggcagcggc gcggcaggcg tccaggtgct
     3421 cgcgcaggcg ctgcaccgct tcctgcagcc ggtcgtcggt gcgcagcggg tgcaacacct
     3481 ccacgcgcag gctctcggtg tcgcgcagca gcacatggaa gggctgggcc tggcgcgtgg
     3541 cctcgctcat gcgcaggcgc agctcgggcg caaagccggt ggccaggatc tgcccggcgc
     3601 agtagctgat ggcgtccagc aggtcgcgct gccaggcgct ggcgccggcg ctgctgtcct
     3661 ccggtgcggg gatcagcacg gccagcaggc gctcgagcag cgccgcgtcc agcgccgcca
     3721 gccagcgcgc gtcgaactca tgcggcaggg ccagcatgaa cagctcggac gcgtccagcg
     3781 tgtcggggct ggtgggcagc agcttgtggc gcaggcgctc ggccagctcg ctggccatgg
     3841 agctgcgctg cgcaaagccg aagtcggcca gcagcgtggt gatgtccagc tgcccggtca
     3901 gcgccgccca ccaggcctgc aggcgcgtgc gggtgtcggc cgactgctcg gcggcctgca
     3961 ccagctgggc cacgcggtcg gtggccgcct gcaccacctg gctgtcgccg cgcacccagt
     4021 ccagcagggc gatgagctgc agatggcgct gtgcggcggc cgcctcgggg tccagggcgg
     4081 ccagcagttg cgccagatcg gcgcgcttgc ggctcaatgc agcacccgtg gcgtggccgg
     4141 ccggcccggg tcgccgccgg agtcggcctc gagcaggaac agggcgatgg ggcagtcgaa
     4201 ctgctcgccg tcctcggcca cgcacaggta gctgccatgc atgctgccgc tggccgtgcg
     4261 caggcggcag ccgctggtgt attcaaaggc ctcgccgggc ttgagcagcg gctgctggcc
     4321 gaccacgccc aggcccttga cctcctgccg gtggccgttg gcgtcgcaga tgatccagtg
     4381 gcgcgcgatc agctgcgcgg cggtgtcgcc ggtgttgctg atggtgatgg tgtaggcaaa
     4441 gccgtacgcg tcctcgtcgg ggctggactg ctcgggcagg tactggggtt gcacctctac
     4501 ggtgaattgg tacttgggca tggcgcaatg gtagctttgc cagctgcgac aatgcgcgcc
     4561 atgagccgca cctaccgcat cgccccctcc atcctgtccg ccgactttgc ccgtctgggt
     4621 gaggaagtgc gcaatgtcgt cgccgccggc gccgactgga tccacttcga cgtgatggac
     4681 aaccactatg tgcccaacct gaccttcggc cccatggtct gccaggcgct caagccccat
     4741 gctgtcacgc ctgacggccg gcccgtgccg atcgacgtgc acctgatggt gcagcccgtg
     4801 gacgcgctgg ccgaggcctt cgcccaggcc ggcgccgacc tgatcagctt ccaccccgac
     4861 gcatcggcgc atgtgcaccg cagcatccag gccatcaagg cccagggctg ccaggccggc
     4921 ctgaccttca acccggccat gtcgctggat gtgctggact gggtgatcga ggacatcgac
     4981 ctgatcctgc tcatgagcgt caaccccggt tttggcgggc agtcatttat tgatagcacg
     5041 ttgcgcaagg tggaagcggc ccgcaggcgt attgaagcct ctggcaagga cattcgcctg
     5101 gaggtggacg gcggcatcaa ggcagacaac atccgccgcg tggccgacgc cggcgccgac
     5161 accttcgtcg ccggcagcgc catcttcggc aagcccgatt acaaggccgt catagacgcc
     5221 atgcgccggg ccctggcatg acggcgccgg tcctgcaggg cctggacgcg gccatcatcg
     5281 acctggacgg caccatggtg gacaccctgg gcgacttcgc ccaggcgctg ggcgccatgc
     5341 tggacgacct ggggctgccc gccatagagc ccgcgtacat cgcgcagatg gtgggcaagg
     5401 gcagcgagca tttgctgcgt tctgtgctca atcatgcgct gacgcaagca gggcaagcgc
     5461 cagatgcttc caaaatcgaa gcgttctatc ccaacgcctg ggagcgctac cagcagcatt
     5521 acctggccat caacgggcgt cattccagcg tctaccccgg cgtgcgccag ggcctgatgg
     5581 cgctgcgcgg cgccgggctg cgcctggcct gcctgaccaa caagcccacc agctttgccc
     5641 tgccgctctt gcgcgccaag ggcctggacg gtttcttcga gcatgtgttc ggcggcgacg
     5701 ccttcgagcg caagaagccc gacccgctgc ccgtcctcaa gacctgcgcg gccctgggca
     5761 cgctgccggc gcgcacgctg atggtcggcg actccagcaa cgacgcggct gccgcgcgtg
     5821 ccgccggctg cccggtggcc ctggtcacct acggctacaa ccacggccag ccggtgcgcg
     5881 cggtggatgc cgactgttac gtggacgcgc tgcacgaact tatgcccaag tgggtgtgcg
     5941 ggctcaggtg aaccgggttg cgcccgctgt gcggggagct gaggccatga agaccaaaag
     6001 ggcccggcac gagcgtgcag ggccccggtg catgcggctt gtcagcgcgc tgccggcttg
     6061 ggcttaaaaa ctatcccagt catcatcgcc ccccgccgcc acggccgcgg ggctggcctt
     6121 gctgggtgcg ctcagaacgg cggcgggctt gctggcggcg cgtgccacgg gcttgggggc
     6181 ggtagccggg cgcggcgcca cgcgtggcgc cggcgccggc ctggcctggc tggcagcgga
     6241 tgctttgccc gtggtgatgc cggcatggcc cgccagcttg aacacggcca cggcctgcac
     6301 caggtcgccg gcctgctggt tcaggctgct ggcggcggcc gagctttcct ccaccagcgc
     6361 ggcgttttgc tgcgtggcct ggtccatctg cgtgatggcc tcgccgacct gggccacgcc
     6421 cgagctttgc tcgcggcttg cggcgctgat ctcgcccatg atgtcggtga cgcggcgaat
     6481 agcgttcacc acctcggtca tggtgttgcc ggccttgtcc accagctgcg tgccctgttc
     6541 cacccgcccc acgctgtcgg tgatcagttg cttgatctcc ttggcagcct ctgcgctgcg
     6601 ctgcgccagc gagcgcacct cgccggccac cacggcaaag ccgcggccct gctcgcccgc
     6661 gcgcgcggcc tcgaccgcgg cgttcagtgc caggatgttg gtctggaagg caatgccgtc
     6721 gatgacgccg atgatgtcgg cgatcttgcg gctggcatcg ttgatgccgc gcatggtgtc
     6781 caccacctcg gccaccaccg cgccgccctg tacggcgacg gtgctggcgt tcatggccag
     6841 ctggttggcc tgggcggcgt tgtcggcgtt ctggcgcacg gtggcgccca gctgctccat
     6901 ggaggctgcc gtttgctcca gcgcgctggc ctgggattcg gtgcgcgccg acaggtcatg
     6961 gttgccctgg gcgatctggg cactggccgt ggccacgctc tcggcattgc cgcgcacgcc
     7021 ggcgaccacg cgggtcaggc tggcctgcat ctcacccagc gcatgcagca ggttcgcggc
     7081 ctcgtcacgg ccctgggccg ctatgggctg ggtcaggtcg ccctcggcaa tctggccggc
     7141 gcggcgcgcg gccagctgca gcggcgcggt gatggatcgg ctgagcagcc aggccaggcc
     7201 ggcgcccagc agcagggcca gtgccgcggc tgcgatgagg atcatgcgcc cctgggcggc
     7261 ctcggccgtg gcctggtcac gggtacgctc atacagcatc agctggcgct cttcgagggt
     7321 gcgcagtgtg gcaatgtagg cgtccgacag gggcttgagc tggctgtcca ccagcgggcc
     7381 cacgtcctcg cccgcggcct tgcgcttgaa caggtcggca cgcggcttgc ggtaggcctc
     7441 gcgcgccttg tcgatgtcgg cgatctggcg ccggccctcg tcggtctcga gcagccgctc
     7501 caccgtcttg cgcgagacgt ccacgccctt ggaggtggca tccatctccg cctgccattg
     7561 cgccaggtgg ctgctgtcgg cattgagcag ggcggcgcgc gtgcgtatcc agttctggtc
     7621 tatgccctgg cgccactgca cggcggcgcg caggcgctcg ttgtcggtgc tggtcagccg
     7681 ctgggtcgcg gccgccagat cctgtaggcg ccacacgccc acggcggcga tcactgcggt
     7741 gatcagcagc accaggccaa aggacagggc caggcgcggg cctaccttga tgtcgttcag
     7801 tcgcatggaa tacctattaa ttcaagtgtg aaacgaaagt aaatgtatca acaagccttg
     7861 gcgctgtcta tcgacaactt cggcagaagc tgcgggtgct acaggccttt ttggatcttt
     7921 ggcgggccgc ggcggggcct ttgctacact ttgccgccat gttcattcac cgcaccgtta
     7981 ttgcaggtcg cagcgaccgg ggagcctggc cctggcgcaa gccagcgtcg cttgcctcct
     8041 gacagcacct gggcaccgcc ccgcgtgcgc ccgtgatccc cagcgcatgg gggatcgtct
     8101 cccgaatgaa cccgcggccc gcacccctgc agcgcgccgc acgagctggg aactcttgtc
     8161 gtgatcactg aactggaatt caaaagcctg gccctggagg gctacaaccg catcccgctg
     8221 atggccgagg cctttgcgga tctggaaacc ccgctgtcgc tgtacctgaa gctcgcccat
     8281 ggcaagggcg atggccggca cagctttttg cttgaatcgg tggtgggtgg cgagcgcttc
     8341 gggcgctata gcttcatcgg cctgccggcg cgcacgctgt tgcgcgccag cggcttcggc
     8401 gccgcggcgc gcaccgaggt ggtgaccgac ggtcaggtgg tggagagcgc cggcggcaac
     8461 ccgctggact tcatcgccgc ctaccaaaag cgcttcaagg tggcgctgcg cccgggcctg
     8521 ccgcgtttct gcggtggcct ggcgggctac tttggctann nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnag cgcagctttg ccaaggcgga ctacctggcg gccgtggagc
     8701 aggccaagga gctgatcgcc gccggcgact tcatgcaggt gcaggtgggc cagcgcatcc
     8761 agaagcgcta caccgaaagc ccgctgtcgc tgtaccgcgc gctgcgctcg ctcaacccca
     8821 gcccctacat gtactactac cactttggcg atttccaggt ggtgggggcc agcccggaga
     8881 tcctggtgcg ccaggaggcc gtggaggagg gcaccaaggt cacgatacgc ccgctggccg
     8941 gcacgcggcc gcgcggcgcc acgcccgaga aggacaaggc ggccgagctg gagctgatgg
     9001 gcgacccgaa ggagcgcgcc gagcatgtga tgctcatcga cctggcgcgc aacgacatcg
     9061 gccgcatcgc caagaccggc acggtgaagg tcaccgaggc cttctgcata gagcgctaca
     9121 gccatgtgat gcatatcgtc agcaacgtgg agggcatcct gaacgagggc atgaccagca
     9181 tggacgtact caaggccacc ttcccggcgg gcacgctcac cggcgcgccc aaggtgcacg
     9241 ccatggagct gatcgaccgg ttggagccga ccaagcgcgg cctgtacggc ggcgcctgcg
     9301 ggtatttgag ctacgccggc gacatggacg tggccattgc catccgcacg ggcatcgtca
     9361 aggacggcat gctgtatgtg caggcggcgg ccggcgtcgt ggcggactcg gtgcccgagc
     9421 tggaatggaa agaaacggaa cacaaggccc gcgccctgct gcgtgcggcg gaactggtag
     9481 aggaaggttt ggaatgacgc gcgccataga tcacgttcag cactgcacca cgatggagga
     9541 tgtgcgccgc ttcatcgatg cgctcgatga tgtgctcgtg cccctgctgg tcacgcgtgg
     9601 cggctacatg acgcaggcgg cacgcatcaa acagtgcaac acccaggtgc gtgacgagga
     9661 ccgcatcgag gccatcgtgg cccgcgtgcg cgcacgcgcc gcgaccgagg gcggccagcc
     9721 cgaggtgatg gaggctatct accgcagcat gatggaggcc tacatcgcct acgaacaccg
     9781 tgagttcgcg cgcatgcgcg caccgggaga tgagtcatga agctcttgat ggtcgataac
     9841 tacgacagct tcacctacaa catcgtccag tactttggcg agctgggcgc cgaggtggag
     9901 gtgtttcgca acgacgagat caccctggag ggcattgccg cgcgcgcgcc cgatcggctg
     9961 gtgatctcgc ccggcccctg ctcgccgctg gaggccggca tctcggtggc cgcgatacag
    10021 cactttgccg gcaggctgcc catcctgggc gtgtgcctgg gccaccagag cattggcgcg
    10081 gcctttggcg gcaagatcat tcgtgcgcag gagctgatgc acggcaagac cagcgtcatc
    10141 actaccgacg agcaaggcgt gttcgccggc ctgccgcggc agttcaccgt gaaccgttac
    10201 cactcgctgg cgatagagcg cgccagctgc cccgaggtgc tggccgtcac cgcctggacg
    10261 gaggacggcg agatcatggg cgtgcgccac aaagagctgc ccatagaggg cgtgcagttc
    10321 caccccgaga gcatcctgac cgagcatggc catgccatgc tgaagaactt cctggagcag
    10381 cgcgcttgat cgacgcgcag cagctgctgc tgttcatcgc cgccggctgg ctgctcaacc
    10441 tgacgccggg ccccgacgtg ctctacatcg tcacgcgtgc gctgaagaac gggctgcgcg
    10501 ccggcctggt ggcgggcctg ggcattgccg ccggctgcct ggtgcatgtg ggtgccgccg
    10561 ccgtgggcgt gggcgcgctg ctggcggcgt ccagcacggc gttcacgctg ctcaagtggc
    10621 tgggcgcggc ctatctgctg tggctgggcc tgcgcctgct gcgcgcgcgc ggcgacggtg
    10681 cgctggccgg cgtggtgcgc gagacagccg gtcacgcagc ggtgccggtc gcgctcaagg
    10741 aggtattcct gggcggcttc tggacgaatg tgctgaatcc caaggtggcc atcttctttc
    10801 tggcctttgt gccgcagttc attgcccccg gcgccggcaa caaggcgctg gcctttgtgc
    10861 tgctgggcgt gctgttcacc gtcaacgcca ttccggtcaa tgcgctgtgg gcgctggcgg
    10921 cggcctggct ggtgcgccgt gtcggcgcgg tgcagcgcgg catgcattgg ctcgaccgcg
    10981 ttgccggcct gctgttcata ggctttggcg tgcgtctggc gctgaccgac gcgcctgccg
    11041 tttgacaacg acatctggag actccatgtc catcaccccg caagaggcgc tgcagcgcac
    11101 catcgagcac cgcgagatct ttcacgacga gatgctgcac ctgatgcgca tgatcatgag
    11161 cggcgagctc tcgcccgtca tgaccgctgc catcgtcacc ggcctgcgcg tgaagaagga
    11221 aaccattggc gagatcaccg ccgcggcgca ggtcatgcgc gagttctcgc gcaaggtcag
    11281 cgtggccgac accacgcacc tggtggacat cgtgggcacg ggcggcgacg gtgccggcac
    11341 cttcaacatc tccacctgcg ccaccttcgt catcgccgcc gccggcgcca aggtcagcaa
    11401 gcatggcggg cgcagtgtca gcagcacatc gggcagcgcc gacgccatgg aggcgctggg
    11461 cgtgaacata cagctcacgc cggcgcagat cgcacagtgc atccaggagg tgggcatagg
    11521 cttcatgttc gcgccgaatc accacccggc catgaagaac gtggccccgg tgaggaagga
    11581 gctgggcgtg cgcaccatct tcaacatcct ggggccgctg accaacccgg cgagcgcgcc
    11641 caacatcctg atgggcgtgt tccacgaaga cctggtgggc atacaggtgc gcgcgctgca
    11701 gcgcctgggc gcgcagcatg cgctggtggt ctatggccgc gacgggctcg atgagatcag
    11761 cctgggcgcc ggcaccctgg tgggcgagct caaggacggc gtggtgcgcg agtacgagat
    11821 ccaccccgag gacttcggcc tgcgcatggc cggcacgcgc gcgctgcgcg tcgagaaccc
    11881 cgaccagtcc aaggccatgc tgctcgccgt gctgcagggc gaagaaggcc cggcgcgcga
    11941 catcgtctgc ctgaacgccg gcgccgcgct gtatgcggcg aacgtggcac ccaccatggc
    12001 cgaggggctg cagcgcgcgc agcaggcgct ggccagcggc gcagccctgg ccaagctgca
    12061 cgagctcgtt gcctacacgc aaaagctcgc ggcttgaggc tgcgccatga gcctgctgga
    12121 cgcccccatc tggaacgatg ccggcacctg gatcgtgctc ggcgtgtcgc tgctgttcat
    12181 cgttgtcggc ctggtgatgc accaggtcat ccgccgggtg ctgcgcaagc cccccgaaca
    12241 ttgaccattg cattggaaaa cccgccatgt ccgacatcct gaaaaaaatc gtcgatgtga
    12301 agttcgaaga ggtggccgcg gccaagcggc gcatgcccct ggccgacatg cgccgcgacg
    12361 ccgagagccg cgtgctcacg cgcgacttcg tcggcgcgct gcgcgccaag atcacggccg
    12421 gccaggccgc ggtgatcgcc gagatcaaga aggccagccc gagcaagggc gtgatccgcg
    12481 ccgatttcat cccagccgac atcgcgcaaa gctatgccga gggcgacggc aaggtcagtg
    12541 ccgcctgcct gtcggtgctc accgaccgcc agttcttcca gggccagccc gactacctga
    12601 agcaggcgcg cgccagctgt cagctgccgg tgctgcgcaa ggatttcatg gtcgatccct
    12661 accagatcta cgagtcgcgc gccatgggag cggactgcgt gctcctgatc gccgcctgcc
    12721 tcgacgacgg tctgatggcc gagatggagg ccattgcccg cagcctggac atggccgtgc
    12781 tggtggaggt gcatgacgcc cccgagctgc agcgcgccct gaagctgaag acgccgctgg
    12841 tgggcatcaa caaccgcgac ctgcgcagct tcgaggtcag cctgcagacc acgctggcgc
    12901 tgcaaaaaca ggtgcccgcc gaccgtctgc tggtgaccga gtccggcatc ctcgcgcccg
    12961 ccgacgtgcg cctgatgcgc gacgccggcg tgcatgcctt cctggtgggc gaggccttca
    13021 tgcgcgcgag cgaccccggc ctggccttgg cgcgtttatt cgcctgagcg tgctgcgcct
    13081 gctgcgcccc cccgccgatc cccagcgccg tgaatggtgg tggctggcat gggtggcgct
    13141 gctcgccgcg ggtttgctgg tcgccggcct gtggcttgag caccagcgtg tagcggcgcg
    13201 cgagcgtgtc ctgctggcca agcaggcggg ggtgatgcat gacaacctgg ggatgcagct
    13261 ggaggccatc aacaatgcca tgcgccatct ccaggccgac cagcgcaagg gcctggacgg
    13321 ggctgatggc catgcccgcc tcatggagcg catgcacaat tttgccgatg ccatgcctgc
    13381 cgtgcgtacc ttcagcctgc tcgatgccga tggccgggtg ctggcctcca gcgccgccga
    13441 tttggtcggg cgcgacctga gcgggcgtga atatttccag accgccaagc agatcgggca
    13501 gaccgatgcg ctgctcgtgg ggcggccttt cttcggcatg ctgggcggct gggtgctggt
    13561 attgggacgc accgtgcccg gggcggatgg cgcttttggc ggcctgctgg tggcggcgct
    13621 ggatgtggag catttccaga ccctgctgca atccgtgcgc tatgcgccgg acatgcgcgt
    13681 cagcctgagc catggtgacg gcatgcgctt catggtcctg ggggccgaca acgagcccga
    13741 tggcgtgaac ctggcgcagg cgggcaccct gttcacgcgc caccgcgaaa gcaggcaggc
    13801 ggccagcgtg ctgcagggct ttccccagcc tggctacccg ccctatgtga tggccttgca
    13861 taaggtgcaa ccgtccaacc tgcggatgga caaaccgctg gtggtggcgg ccgggcggcc
    13921 gctggacgat gtatttgccg gctggcgcga ccaagcctgg cgctttgcac tggtctacct
    13981 gctcgccttt tcagcggccg gcgtggcgct gcggctcatg caccgtaggc agaatgaagc
    14041 acgcgaccag gcgcagttgc tggaggccgg gcagcgcagg cagcaggaga tgctgcgccg
    14101 gctggtggaa agcctgcccg gcatgctgta ccaataccag ctggagcctg acgggcgcag
    14161 ccacttcccc tacgccagcc ccggcgtgac cgatatctat ggctttgccc ccgagcagct
    14221 gctggccgat gcggcgcccg tattcgcgcg catccacccc gaggacttgc caaagggtca
    14281 ggtcaacatc caggaatcgg cgcgcacgct ggccgactgg aagagcgagt accgcgtcat
    14341 cctgcccggc attggcgagc gctggctcag cggccaggcg aggccgcagc gcctggacgg
    14401 cggcgccgtg ctctggcatg gctacatcca cgacgtgacc gacatgaagc gccaggccct
    14461 gcagctgcag gagaccgagc gcctgctgca gcagctgatc aacgacatgc ccgtgggcct
    14521 gtgcatggtg gacgcgcagc gccgcatcta ctttcgcaac cggcgctttc tggagcattt
    14581 cggctatgcc gaggccgagg tgcccacgct gcacgaatgg gccttgctcg cctacccgga
    14641 cggcgactat cgccagcagg cggcgcatgc ctggggccag gccatggccg gcgcgcgcgc
    14701 gcaagggggg gtgatcgcgg cccagcccta ccgcgtcacg gcgcgcgatg gcgcgcagca
    14761 tgtgatggac atcggcggcc tgctgtttgg cgagcatttc ctggccacct tccaggaccg
    14821 caccgagcag caggcgcaaa gcgagctgct gcaccggctg gcctatgtgg atggcctgac
    14881 cggcatcgcc aaccggcgcc agttcgacca gcagctcgaa gccgaatggc gccgctgccg
    14941 tcgcagcggc aagccgctgg ccgtgctgct gctggacatt gactacttca agcaatacaa
    15001 cgacctgtac ggccaccagc agggcgacga atgcctcaag gccgtggcca ctgcgctgcg
    15061 cggtcagctg gggcgttcgc atgacctggt ggcgcgctac ggcggcgagg aattcgtctg
    15121 cctgctgccc gagtgcgacg ccgccggcgc gctgcacaag gggcaggagc tgtgccatgc
    15181 ggtacaggca ctgggcctgg cgcatgcgca atcgcgggtg gcggatgtgg tcaccatcag
    15241 cgtcggtgtg gcctgccagg tgcccgacgg cgagggcagc ccggcggccc tgctgcagca
    15301 ggccgacatg cagctctacc aggccaaggc gcaggggcgc aaccgcgcgc tgggtgcgca
    15361 ctgactttga tgtcggcgca tgacgccggc gccggcccgc tgcctacaat ggcctgcatc
    15421 gtctccgtgg caggccatag ccacgcccac agcccacgcc gaggtgttct tgcagcccat
    15481 cgcgccgtca catatgcagc gtttccgccg cgaatggtgg tggctgggtc tgggcgcgct
    15541 gctggtgggc gcgatgctgg cgctgggcct atggcatgag cgccagcaga ccgaggcacg
    15601 cgagcgccag cacctggcgc aacaggcgcg catgctgcac gacaacctgg tgccgcagct
    15661 gggcgccatc aaccatgtgc tggccagcct gcaggccgat gtgccgcgct ggcgccagcg
    15721 ccccggcggc atggccgagc tgaaccagcg cctgcagctg ttttttgcgg ccctgccggg
    15781 tgtgcgcagc ctgaatgtgc tcgatgggca ggggcgggta ctggcctcca gccatgacga
    15841 gttgctggga caggactttg gccggcgtga ctactttcag atggcgcgca ccggcatggg
    15901 gcccgagacg ctgctggtga gccggccctt ccagggcgcg ctgggcggct gggtgctggt
    15961 gctggggcgt acgctgacgg ctgcggacgg cagcttcgat ggcctgctga cggcctcgct
    16021 gaaccccgag tacctgcatg tggtgctgca gtcggtgcgc tacgccagtg atctgttcac
    16081 ctcgctcacg catggcgacg gccagcgcct gatggcgctg ggcgacgcca atgagcgcgc
    16141 tggcgacaac ctggccatgc ctggcagcct gttcatgcag catctgcaga gccagcgcag
    16201 cgacaacctg ctgcgcggcc cgctgcgccc cggcggtgcg gactacctgg tggccatgcg
    16261 caacctgcaa ccacccgggc tgcacatggg caagccgctg gtcgtgggcg tggggcgtac
    16321 cacgcaggcg atgtgggcgc agtggcgcca gcgggcgcgt tttctggcgc tggtgtatct
    16381 gctgtcttgc gcagcgctgg ccggcggcct ggcggctctg cagcgccggc aatggcggct
    16441 atggcacacg caggagctgc tggagcgcca gcaggccctg cagcgccagc aggaggacat
    16501 gctgcgccgc ctggcggaca acctgcccgg gctcttgtac cagtaccagc tggagcccga
    16561 cggccgcagc catttcccct acgccagtcc gggcgtgacc gatgttttcg gccatccgcc
    16621 cgagtccttg cgcgctgacg ccgggcccgc cctggcgcgc atccacccgg cggatcgggt
    16681 cgcgggcgag gcggccatgc aggagtcggc gcgcacgctg ggcgtctggc agggcgagta
    16741 ccgcgtgacc ctgcccggcc gtggcgagcg ctggctcagc ggccaggcgc ggccgcagcg
    16801 gctgcgcagc ggcgccgtgc tttggcatgg ctatgtgcat gacatcaccg aggccaagcg
    16861 ccaggccatg gagctgcagg agaaggagcg gctgctgcgc cacctgatgg agcaaatgcc
    16921 catcgggctg tgcatcgtcg atgagtccgg gcatttcagc tttcgcaacc agcgctttgc
    16981 cgagtacttc ggctatccgg cgagcgagcc gctgacgctg cagcgctggt ggcaggaggt
    17041 ctaccccgac gccgactacc gcgcccgcgt gctggcgctc tggaacgaag ccatggacca
    17101 tgccgacgca catgacggca ccctacccgg ccatgagtac cgcgtgacca cgcgctacgg
    17161 cacgcagcgc atcatggcca ttggcggcat agcgatgggc gggcgcttca tggccagctt
    17221 catcgacctg accgagcaaa aggcgcacag cgacctgctg cggcatctgg cctttgtcga
    17281 tggcgtcacc ggcattgcca accgccggcg ctttgacgag gccttgcagc gtgaatggcg
    17341 cgactgcctg cgcaagggca agccactggc gctgctgatg ttcgacatcg accatttcaa
    17401 ggcctataac gacttttacg gccaccagcg cggcgacggc tgcatcaagg ccgtggcggc
    17461 ggtgctgcgc gccggcctgg gccgcccgca tgatctggcg gcgcgctatg gcggcgagga
    17521 attcgtctgc ctgctgcccg actgcgggcc acaggacgcc cgcgccaagg cgcaggagct
    17581 gtgccaggcc gtgcgcagcc tgggcctcgc gcacagtgcc tcgcccgtgg cggcggtggt
    17641 cacgctgagt gccggcgtgg cctgcctggt gcccgacgag cgcaacagcc ccgaggcgct
    17701 gctggcgcgt gccgacgcca acctctaccg cgccaagtct gccgggcgcg accgtgtcga
    17761 tcagggtgtt gaaacactac aattacaata gctgtttgcg ctttattgac gggcgttata
    17821 ggttgattta atgcttgaaa acatgcatgc cgaagcggcc acgcagctgc gcagcgccaa
    17881 cccggacgac tggcccgtgg cgccgggctg gcaggccctg acggcgggct tttttgccag
    17941 cgccgaaggc cgctcgctgc tggactttct gcgccagcgg ctgcaggccg gggcggtgct
    18001 gtttccgccc gagccgctgc gcgcgctggc gctcacgccg cccgaggccg tgcgcgtgct
    18061 catcctgggg caagacccct accacggccg cggccaggcc gaggggctgg cgttctcggt
    18121 cgcgcccggc gtgcgcctgc cgccctcgct gcgcaatatc ttcaaggaaa tgcagcgtga
    18181 cctgggccag cccttcccgc ccttgcccga tcccggtggc agcctggtgt catgggccag
    18241 gaacggcgtg ctgctgctca acacctgcct gacggtggag gagggccagg ccgccagcca
    18301 ttccggccgc ggctgggagc gcctgaccga cgccgtgatc cgccatgtgg ccgaagggcc
    18361 gcgcccggtg gtcttcatgc tctggggcgg ccatgcgcag ggcaagcgcg cgctgattcc
    18421 cgccgatcgc gggcatctgg tgctcacctc caaccacccc tcgccgctgt cggcgctgcg
    18481 cccgcccgcg ccctttatcg gcaacggcca tttcggccag gcgcgcgcgt ttcgcgaacg
    18541 gcaaggggct tgacgcaaac ccgcaccatg cacaacgcac agaccctcga ctggaccctg
    18601 ccctatgcct cgcagcgcag cgcggtgctg gggcgcaacg tggtcgccac ctcgcagccg
    18661 ctggcggccc aggccggcct ggccatgctg caggccggcg gcaacgcggt ggacgcggcc
    18721 attgccgccg ccatggcgct gacagtggtc gagcccgcgg gctgcggcat cggcagcgac
    18781 ggtttcgcca tctgctggga cggcaggcag ctgcacggcc tgaatgcctc gggccgctcg
    18841 cccgcagcct ggacgccgga atactttgcg cgcctgggcg gcattcccga gaagggctgg
    18901 aacgccgtca ccgtgcccgg cgccgtatcc gcctgggtgg cgctgtccaa acgcctgggc
    18961 cggctgccgt ttgcccggct ggcccagccg gccatcgatt acgcgcgcca tggcattccg
    19021 gtctcgccga cgattgcgcg cctgtgggaa ctcggcgccg ccaagttggg cgaccagccc
    19081 ggctttgccg aatgcttcct gcccgccggc cggccgctgc gcgccggcga gatcttccgc
    19141 agcgaggccc atgcacgcac gctggagctg atcgccgcga ccgagggcga ggccttctac
    19201 cgcggcgagc tggccgagaa gatggccgcg cacgcccagg cccatggcgg cgcgatgacc
    19261 gtcgaagacc tggccgcgca ccagcccgat tgggtgggca cggtgagcca gcgctttggc
    19321 gattcggtga tccacgagat tccgccgaat ggccagggca tcgccgcgct gatggcgctg
    19381 ggcatgctcg atgaacatgg cgtgggcgcc cacccggtgg acgatgtacg gacggtgcac
    19441 gccagcatcg aggccatgaa gctggccttt gccgacctgc accagtacaa cgccgacatc
    19501 gactacatgc gggtcgcgcc ctcggagctg ctgaaccccg cctacctgaa gggccgcgcg
    19561 gccctgatcg acccggcgcg cgccggcgcc ccgagctatg gcgcgccgcg ccccggcggc
    19621 acggtgtacc tggcggcggc cgacgcctcc ggcatgatgg tctcgttcat ccagtccaac
    19681 tacatgggtt ttggctcggg cgtggtggtg ccgggcacgg gcatcagcct gcaaaaccgc
    19741 ggtcatggtt tcaacctggt gccgggccat gccaaccagg tggggccggg caagcggccc
    19801 tcgcacacca tcatcccggc ctttgccatg cacgccgacg gcaggccgca aatggccttt
    19861 ggcgtcatgg gcgggcccat gcagagccag ggccatgtgc agatggcgct gcgcgtgctg
    19921 cgctatggcc agaaccccca ggccgccgcc gacgcgccgc gctggcgcgt gaccgggggc
    19981 aagggcgtgg ccgtggagcc gcatttcgac gccgccgtgg tggccgggct gcgcgcgctg
    20041 ggccatgagg tgacggtgga ggcgggcaac ggcgtgttcg ccttcggcgg cgcgcagctg
    20101 gtgctgcgcc agggcgatgt ctacatcgcc ggctcggatt cgcgcaagga tggttgcgcg
    20161 gtggcgttct gagcaaaaac gggctcaatc gcttaccaat caagcgcaag cagctatcgt
    20221 ttttgcgtga ttgtgcactc ctatttttta tagcgtgtca cgcaatacca gcaccgcttt
    20281 gtggctgttt ctccctctcc cgtgcgcgcg ggagagggcg ggggtgaggg cgccaagaag
    20341 gggtgcggcg cttcatcacc ccctcacccc gaccctctcc cccaaggggg cgagggagtt
    20401 aaaaaccggc gccagataag cgttgcgtgc tatcggaaac aagccgaaag gggggtgggc
    20461 cagggcggtg catccgaggc tcgtttccaa cctgtgttct tgaggctcaa cgcgcaccgg
    20521 tgcgctccgg ctccggcgct ggcagccaca gctccagcgt gcccggctgg cccttgaggc
    20581 cagtgggcag gccggtgacc cagacctttt gccggctggc cggcgaccag cgcggctgca
    20641 ccagctgcgc cagggcctgg gcgcaggggc gtagccgggc ctggtggacg acgaaggtcg
    20701 gttgctgcca gggcacgggc gggcgctgct ggcgcatggc ggcggtgcgc gccacgtttt
    20761 ctatgcgcgg gatgcgcagc gcgccggcgc cggcctgctg caggcgctgg cgcagctgct
    20821 cggcacgctc gcgcgtcgct tcgtcgtaga tctgcgtgta cagcagggtg ccggccgggc
    20881 agtcgtcgcg caggcccgcc gcctcggcgg cgctgatggc ggtgatcagt tcgggcatgg
    20941 cgccggcggc gggcgcctcg gtgctggtcg c
//
LOCUS       DAOMTQ010000037        20816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_302_length_20816_cov_27.371177, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000037 DAOMTQ010000000
VERSION     DAOMTQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 20816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20816
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_302_length_20816_cov_27.371177"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..85)
                     /locus_tag="PK518_05580"
     tRNA            complement(9..85)
                     /locus_tag="PK518_05580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Pro,seq:cgg)
     gene            complement(101..1084)
                     /locus_tag="PK518_05585"
     CDS             complement(101..1084)
                     /locus_tag="PK518_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRM48418.1"
                     /translation="MAANSPSTAATLALIANDMQEVDKVIASRLQSSVPLVGDVARYI
                     ISAGGKRLRPALLLLVSGALGYQGAQRFNLAAVVEFIHTATLLHDDVVDESTLRRGRA
                     TANESFGNPASVLVGDFLYSRAFQMMVDTGSLRVMQVLADATNVIAEGEVQQLMNTHD
                     ASLDEAGYLNVIRSKTAKLFEASARLAAILAGSTAEIEQACADYGQALGTAFQVIDDV
                     LDYDGDAQEMGKNLGDDLREGKVTLPVIIAMQRGTAEQHELLRNVIETGSTDELQRVA
                     AIIRDTGALSATRDAAAAQARLAMDAAAKLPTNSYTEGLLQLAAQLLERRT"
     gene            1263..1574
                     /gene="rplU"
                     /locus_tag="PK518_05590"
     CDS             1263..1574
                     /gene="rplU"
                     /locus_tag="PK518_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRM48419.1"
                     /translation="MYAVIKTGGKQYRVAVGEKIKVEQIAADVGQEIVIDQVLAVGNG
                     AELKVGTPLVSGAAVKATIVAHGKHDKVRIFKMRRRKHYQKRQGHRQQFTELQIGAIT
                     A"
     gene            1590..1847
                     /gene="rpmA"
                     /locus_tag="PK518_05595"
     CDS             1590..1847
                     /gene="rpmA"
                     /locus_tag="PK518_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRM48420.1"
                     /translation="MAQKKGGGSTRNGRDSKPKMLGVKAYGGELISAGSIIVRQRGTR
                     IHPGVNVGVGKDHTLFALVDGHVSFGTKGALSKHTVSVTPA"
     gene            2001..3074
                     /gene="obgE"
                     /locus_tag="PK518_05600"
     CDS             2001..3074
                     /gene="obgE"
                     /locus_tag="PK518_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRM48421.1"
                     /translation="MKFVDEAFIDIAAGDGGNGCVSFRHEKYKEFGGPDGGDGGRGGH
                     VFAVADPNLNTLVDFRFSRRHDAKRGEHGKGSDMFGAAGSDITLKMPVGTIISDANTG
                     EQLYELLTPGEVITIAKGGDGGFGNLRFKSAINRAPRQKTPGWPGERKNLKLELKVLA
                     DVGLLGMPNAGKSTFIAAVSNARPKIADYPFTTLYPNLGVVRVGPEQSFVVADIPGLI
                     EGASEGAGLGHQFLRHLQRTRLLLHVVDMAPFDDAVDPVAQAKAIVAELKKYDEGLHG
                     KPRWLVLNKLDMVPAEERAVRVKDFVKRFKWKGPVYEISALTREGCDPLVKAIFQHVH
                     AEQLAQNAPAEVDPRFAGEHGQD"
     gene            3149..4288
                     /gene="proB"
                     /locus_tag="PK518_05605"
     CDS             3149..4288
                     /gene="proB"
                     /locus_tag="PK518_05605"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRM48422.1"
                     /translation="MVSSVLRDARRIVVKVGSSLVTNEGRGLDEVAIGEWSRQLAALV
                     RGEGGVAREVIMVSSGAIAEGMKRLGWSSRPSEIHELQAAAAVGQMGLAQMYETKLRE
                     QAMGSAQVLLTHADLADRERYLNARSTLLTLLELGVVPVINENDTVVTDEIKFGDNDT
                     LGALVANLVEADVLVILTDQKGLYTADPRRDPQAQFVHEAKAGDPALEVMAGGAGSSI
                     GKGGMITKIVAAKRAAGSGASTVIAWGREPDVLLRLARGEAIGTLLVAQTQKKQARKR
                     WMVDHLQLRGSVTVDAGAAAKLRGEGKSLLPIGMVAVEGDFARGDVIAVRDAGGTEIA
                     RGLANYASAEARRLCRKPSSEFERLLGYAAEPEMVHRDNMVVFTA"
     gene            complement(4326..5012)
                     /locus_tag="PK518_05610"
     CDS             complement(4326..5012)
                     /locus_tag="PK518_05610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRM48423.1"
                     /translation="MLDRDGFRPNVGIILLNQRNQVFWGKRIRTHSWQFPQGGIDRGE
                     NPEQAMFRELHEEVGLQPSQVRVIARTRDWLRYEVPDRYIRRDARGHYRGQKQIWYLL
                     QLLGHDWDLNLRATDHPEFDAWRWNDYWVPLDVVVEFKRGVYEMALTELARFLPRFEQ
                     RNRYLRSGMRAREAEGGQPAMHRSVSMMLKPGMELPPGASFDPDPQNSMPAPLQEAAP
                     ADQYNKPASR"
     gene            5103..6848
                     /locus_tag="PK518_05615"
     CDS             5103..6848
                     /locus_tag="PK518_05615"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRM48424.1"
                     /translation="MKASQFFISTLKEAPADAEVASHKLMMRAGLIKKLGAGIYNYMP
                     MGLRVIRKVESIVREEMNRAGAVECTMPVVQPAELWQETGRFDKMGPELLRIQDRHGR
                     DFIIQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
                     YSFDRDQAAAKASYQVMAQAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGE
                     DAIVYCPGSDYAANMEKAEALAPAGPRLAAAAPMTRTPTPGKATCAQVAELLNVPLAT
                     TVKSLVLATDETNEAGEVVQSQVWLLLLRGDHDMNEIKVSKVSGLDKGFRFATVAEIE
                     AHFGAKPGYLGPIGLKQSVRIVADREVAVMADWICGANEEDYHITGVNWGRDLPEPDA
                     VADLRNVVAGDPSPDGKGELAIERGIEVGHVFYLGTKYSRAMDATFLGEDGKPAFFEM
                     GCYGIGITRLPAAAIEQNHDERGIIWPDAIAPFTVVICPIGMDRSQEVRAAAEKLYGE
                     LLAAGVDVILDDRGERPGAMFADWELIGVPHRITIGDKGLKEGQVEYQHRRDSAATKL
                     GVAEVFAYVKERVKV"
     gene            6845..7507
                     /locus_tag="PK518_05620"
     CDS             6845..7507
                     /locus_tag="PK518_05620"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRM48425.1"
                     /translation="MTRGGDSASGLSRRACLASLAVGPSAWLGLPAAAHAGGQLEEPL
                     MDSVRMALSSAVADLAPPEPLFVTTESRLTYLRWLGTMSDRLRRSKPDWEVRRDFLQT
                     VWYESKRAGLDVSLVMGLIQVESAFRKFAVSSVGARGYMQVMPFWTRAIGDGDAGKLF
                     QMQTNLRFGCVILRHYLDRERGDLFMALGRYNGSRGKPPYPNAVFAAQSRWAFQDRAA
                     SA"
     gene            complement(7483..8814)
                     /locus_tag="PK518_05625"
     CDS             complement(7483..8814)
                     /locus_tag="PK518_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM48426.1"
                     /translation="MSLTHSLLLIALLIAASAFFSVAEISLAASRRLRLRQMADEGDT
                     RADRVLLMQDQPGEYFTVVQIGLNAVAILGGIIGEGALSPYFNALFALWLPEKSAQTL
                     GFVGSFLAVTSLFILFADLLPKRLGMAHPEVVALRVVRPMQGCMALFKPIVWLYSRCA
                     DALLRLMGLSSQRDERITPEDILAMTEAGARAGVLALPEQQVIANVFELDTRSVTSAM
                     TLRERIAFFLRDDPDELIRARIAAEPFSTYPVCDGDIDHVVGYVDAKDLFQRVLNNQP
                     ISLADDQLVRKVLIVPDRLSLAEVLAHFRQVHEDFAVIVNEYSRVVGVVTLNDVMSTV
                     MGDLVGPADEEQIVRRDENSWLIDGTAPIEDVLHVLGLDELPHSEDYETLAGFLMVML
                     RRVPRRTDSVTVGHYKFEVLDVDSYRIDQVMVSRLLTAPAENGAQAEAARS"
     gene            complement(8876..9883)
                     /locus_tag="PK518_05630"
     CDS             complement(8876..9883)
                     /locus_tag="PK518_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM48427.1"
                     /translation="MTSHCLVPQAPALSLPVAQMRSVFHPADVARKLARLQDAGAQRD
                     HEPLRTVYERMLERGPERFQVKPSGLPDMAALYEQLPNFTEVLDDVRRQVALAQGSSD
                     GLEVTPMLLLGAPGIGKTHFARQLAALLGTGMNLVPMSSMTAGWLLSGSSAQWKGARP
                     GKVFEALVEGEYANPVIVVDEIDKAAGDAQYDPLGALYALLEHDTAQSFTDEFADVAI
                     DASQVIWITTANDERAIPDPILNRMNVFAVQAPTLAQARAIAQRLYQGIRAEHDWGRL
                     FEPEAQADVLDLLAQMPPREMRRALMTGFGNARLEQRERVEVADLPRTAGGKSRIGFL
                     Q"
     gene            complement(10025..10354)
                     /gene="grxD"
                     /locus_tag="PK518_05635"
     CDS             complement(10025..10354)
                     /gene="grxD"
                     /locus_tag="PK518_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRM48428.1"
                     /translation="MSDVKQRIEQLVKTNDVLLFMKGNASFPMCGFSGRAVQILKACG
                     VDPKDIAAVNVLEDDEIRQGIKDFSHWPTIPQLYVKGEFIGGSDIMMEMYESGELQQV
                     LGTKAQG"
     gene            complement(10402..11253)
                     /gene="prmC"
                     /locus_tag="PK518_05640"
     CDS             complement(10402..11253)
                     /gene="prmC"
                     /locus_tag="PK518_05640"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRM48429.1"
                     /translation="MPTTTIAQALARAQAQGLARIDAQMLLLHLLGRPVNDRAWLLTH
                     DGDALAPHDGQRYTALCARRAGGEPVAYLTGQKEFWGLPLAVDARVLDPRPDTETLVA
                     WALEVLQNQHAPRIADLGTGSGAIALALQHERRDASVLAVDASADALAVARANAERLG
                     LPVRLQHGNWLASITGPFDAIVSNPPYIPAQDPHLAALTHEPLSALASGADGLADIRA
                     IIAQAPRALAPGGWLLIEHGWDQAPAVQALLRAAGLAQVQGRNDLAGIARCSGGSMPV
                     GLSPSVK"
     gene            complement(11311..12390)
                     /gene="prfA"
                     /locus_tag="PK518_05645"
     CDS             complement(11311..12390)
                     /gene="prfA"
                     /locus_tag="PK518_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRM48430.1"
                     /translation="MKPFLRSQLERYAQRLQELDFLLSREDIMADMAQYRTISREHAE
                     VTQIAGRYARYQQREADLAAAQELLADPDMAEMAQEEIQGAQDELVALEDELQRLLLP
                     KDPDDARAAFVEIRAGTGGDESALFAADLARMYTRHAANVGWKVEVLSANESELGGYK
                     EVVLRVDGDNAYGALRFESGGHRVQRVPDTETQGRIHTSACTVAVMPEPDAAAAITLN
                     PADLRIDTFRASGAGGQHINKTDSAVRVVHIPTGIVAECQDGRSQHSNKAKALQVLQA
                     RIQEKERSERAAKEAATRKGLIGSGDRSDRIRTYNFPQGRLTDHRINLTLYKLLTIME
                     GDLDEVLQALQHAREAEQLAELGLD"
     gene            complement(12484..13794)
                     /gene="hemA"
                     /locus_tag="PK518_05650"
     CDS             complement(12484..13794)
                     /gene="hemA"
                     /locus_tag="PK518_05650"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRM48431.1"
                     /translation="MAVWALGINHHTAPLDLRGRFAFAIDQIAPTLHGLRQSLASGAS
                     SHPGVETAIISTCNRTEIYCAAHKPALDHTLDWLAASGGVSPSLLRSHSYTLESGLVA
                     RHAFRVASGLDSMVLGEAQILGQMKDAVRAAEGAGALGTTLNQLFQRSFAVAKEVRTS
                     TDIGAHSISMAAAAVRLAGQLFEDLSKIRVLFVGAGEMIELCATHFAAKNPKQISIAN
                     RTLERGEKLATRFGGDVMRLADLPEHLHEYDAVISCTASSLPIIGLGAVERALKKRRH
                     RPIFMVDLAVPRDIEPEVKQLEDVYLYTVDDLAGVVQTAQANRQAAVAQAEAIIDAGV
                     QNFMHWMELRSPASQAGAVVPLIRQLNSQADEWRALEIARAKRLLAKGEDIDVVLEAL
                     SRGLAQKMLHGTMAELRAGDADARAQTAEAVSRLFLRSQSKTGL"
     gene            13868..14146
                     /locus_tag="PK518_05655"
     CDS             13868..14146
                     /locus_tag="PK518_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48432.1"
                     /translation="MSPLAILNHLLNFAAPALALAVLLVLVSHLFMRKTAAAHGWVAP
                     ITINFVVGCAVLVAGLAWLGRDGRMLTYAALVLACASSQWLMLRAWRG"
     gene            complement(14154..16619)
                     /locus_tag="PK518_05660"
     CDS             complement(14154..16619)
                     /locus_tag="PK518_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HRM48433.1"
                     /translation="MAHAPRPLARLLCWLSVAWLMALGTPPTLAQGRPASSDAAPTAA
                     ADKPGDNGTPLPQVDALRKQLDAIPLKLGEPDDGRKLLADINAIGQAAERVVSHRGTE
                     LAEIDRRLEGLGPAPGSGAPADAPDVAQQRKLLTQQRSAIDSELKLARLVAVDAEQRS
                     ADLVRQRRAQFRAALTARSDSPLAPTFWRNLRNAAPRDLVRLQALGADLAGAVRATLQ
                     SPRHNAFWAYLALALLLAVGGPWLAERILLRALPPRLPAARLRRTLLASAAIVTHVFI
                     VGTAVQLAWNALQLGAPLGDDLRALQQASLRAVVYASFVIALGHALLSRRRSSWRLLP
                     IADELARRLSPFPWWIASLSALGALVAELNVIVGASLSAEVLVHALFALLLSLTVGKA
                     LGHMRAAPAASDADAGAPHGQGERPLWLGLILAAAGLAVGVAVLAVALGYVALAGMLA
                     RQMVWSGVVFATSYLLMQLGDDLCDTLLSSKGGFGARIHGTLGLDARLLDQAAVLVSG
                     VLRLVLFFYMVVALMAPLGTDPDELFRRGSSVDHSLRIGDITLAPQALLTALAVACAG
                     FLAIRLLKHWLSERFFPHTTLEPGMRTSIVTLLGYAGAVVVVAVALAGLGISVERIAW
                     VASALTVGIGFGLQAIVQNFISGLILLAERPVKVGDWVVLGDTEGDVRRVNVRATEIQ
                     QSDRSTVIVPNSEFITKTVRNVTLDSAQGRVLLRLPAPLDTDARRMRALILQAFEAQE
                     AMLDDPAPSVTLEGIQGGTLTFLAIGYVSSPRQVSGVRSDVLFSILDALREAGLTLSP
                     PATTTVAQPVSEAAPGRSLIERI"
     gene            complement(16692..17450)
                     /gene="ugpQ"
                     /locus_tag="PK518_05665"
     CDS             complement(16692..17450)
                     /gene="ugpQ"
                     /locus_tag="PK518_05665"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRM48434.1"
                     /translation="MSTPLPPWPYPRWIAHRGAGKLAPENTLAAFRLGARHGYRMFEC
                     DAKLSSDGVLFLLHDATLERTTNGHGAAGERSMGELAQLDAGGWHSRAYAGEAMPTLE
                     ALARYCLANGHHLNVEIKPTPGQELATGRAVGELLSRIWPATAVPPLLTSFKPAALTG
                     ARETAPALPRGLLLDKLADGAADVQTALDLGCQAMVLNHALWDAALVARVHGAGLRAL
                     SYTVNDEWAAERLIALNTDGIITDRVDLFSPAAT"
     gene            complement(17451..18458)
                     /gene="ugpC"
                     /locus_tag="PK518_05670"
     CDS             complement(17451..18458)
                     /gene="ugpC"
                     /locus_tag="PK518_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRM48435.1"
                     /translation="MASISLKNIVKRYGSGKSAVPVIHGVNAEIQDGEFIVLVGPSGC
                     GKSTLLRMIAGLEEITGGDLFIGDKRVNSLEPAKRNIAMVFQNYALYPHMSNYENMAY
                     GLKLAKVPADEIRQRVDKAAKILELSHLLERKPRELSGGQRQRVAMGRAIVRQPQVFL
                     FDEPLSNLDAKLRGQTRIEIQKLHAELGITSLFVTHDQVEAMTLAQRMIVMNAGNVEQ
                     FGTPEEVYHQPASTFVAGFIGSPPMNLLKNAPGGQAGRILGIRPEHIDLAASGGWQFQ
                     VETVELLGAERLLYGKVGGEEVTVRVEEGSAYPRPGETAHIVPREDRLHWFSQETGKR
                     I"
     gene            complement(18440..18730)
                     /locus_tag="PK518_05675"
     CDS             complement(18440..18730)
                     /locus_tag="PK518_05675"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRM48436.1"
                     /translation="MSTLALETAPHAVALQCAADALVMTLHCGHILSVPLVWFPRWIR
                     ATAEQLAAFEWLGMGEGIHWPRLDKNISIAGLLEGLTAPNALRLALTWPPSP"
     gene            complement(18893..20467)
                     /locus_tag="PK518_05680"
     CDS             complement(18893..20467)
                     /locus_tag="PK518_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRM48437.1"
                     /translation="MINKKKPWIKARWIGVALLLFATGYLTLNYRILVVGVLYRWAAD
                     DQFNAIWNLVSYASQGLLLFLVIGSISGWAFRSVLLLVFISGTVNLVYQSILYDLINL
                     STFQWMLAESRQSLSALKEFYKDFIWGVTRAGIAVGLFTLTRKAIKYAVFSKMAPHPE
                     NAPILTIVVLMIFLAADPALKALGRDRGAEMNVYGMAYQTMTEEIPNRMPASVKPDFK
                     PSANKIIWLIDESISWRYYDEMFRSEWVSRWGGIDFGEARSLGNCSAQSNAALRWGVN
                     VSKLNASTDLRTNSTVWAYAKQAGFRTILMDGQVHGNPQNYLWPPEKRLIDEVVPMAA
                     GLDSDLKIARRLNGLLKADGRHFAYIVLKGAHYQYFSNYPDGNLDRSLPIEEQYRKAL
                     RYSKGNFLNELLDGVNMRNVVIFYTSDHGQHLGNGVIPHCNNKPHPEELSVPLILFTD
                     PDLRNQFQRNDGEIRSHSQIFPTTLLLMGYAAEYASREYDNPLPTTSKRIVRFGKRIF
                     PSTNADSIEFFFDDSN"
     gene            complement(20574..>20816)
                     /locus_tag="PK518_05685"
     CDS             complement(20574..>20816)
                     /locus_tag="PK518_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="with UgpABC is involved in uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HRM48438.1"
                     /translation="ALFVIQFIYGWNQYLWPLLMTTSEDMYPVVIGIKRMLAGGEAAV
                     DWNIVMATAILAMLPPTLVVILMQKWFVKGLVDTEK"
BASE COUNT         3561 a         6467 c         7015 g         3773 t
ORIGIN      
        1 tttttgtctg gtcggggtga gaggattcga acctccggcc tctacgtccc gaacgtagcg
       61 ctctaccagg ctaagctaca ccccgatgtg ccgtgaccgc ctaggtgcgg cgttcaagca
      121 gctgagccgc taattgtagc aaaccttctg tgtacgaatt ggttggcagt ttcgctgcgg
      181 cgtccatggc caatctggct tgggcggcgg ctgcgtcgcg cgtggctgag agggcgcctg
      241 tatcgcgaat gatggcggcc acgcgttgca gttcgtcggt ggagccggtc tcgatcacgt
      301 tgcgcagcag ttcatgctgc tcggcggtgc cgcgttgcat ggcgatgatg acgggcaggg
      361 tcaccttgcc ttcgcgcagg tcgtcgccca ggtttttgcc catttcctga gcgtcgccgt
      421 cgtagtcaag gacgtcgtcg atgacctgga aggccgtgcc cagggcctgg ccgtagtcgg
      481 cgcaggcctg ttcgatttcc gccgtgctgc cggcaagaat ggccgccaat cgcgcgctgg
      541 cttcaaagag tttggcggtc ttggaacgta tgacgttgag gtagcctgct tcgtccaacg
      601 atgcatcatg ggtgttcatg agctgctgga cttcaccctc ggcaatgacg ttggttgcat
      661 ccgccagaac ctgcatcacg cgcaggctgc cggtgtccac catcatctgg aaggcgcgcg
      721 aatacagaaa atcgcctacc agcacgctgg ccgggttgcc aaagctttca ttggccgtgg
      781 cgcggccgcg gcgcagggtg gattcgtcca ccacatcgtc atgcagcaat gtggcggtat
      841 ggatgaattc gaccacagcc gccaggttga agcgctgcgc cccctggtag cccagggcgc
      901 cgctcaccag cagcagcagg gcggggcgta ggcgcttgcc gccggccgag atgatgtagc
      961 gggcaacgtc tcccaccagt gggacggatg attgaaggcg cgatgcgatg accttgtcca
     1021 cctcttgcat gtcgttggca atcagggcga gggtggcggc ggtgctgggg gagttggcgg
     1081 ccaaggtgga tgctggtggt aaacaaaagc ggcaattata ggaagccgcc cgaagcattc
     1141 gcggcgccac gcggcttgca cgggaggcgg gagcctgaat ttgcggaatg tacaaacgcg
     1201 cagtacaatc gacggttctg tggaaatccg tttcacagaa ctccaactca gatgaggttt
     1261 acatgtacgc ggtcataaaa accggtggca agcaatatcg cgttgctgtc ggcgaaaaaa
     1321 ttaaagtaga acagattgct gcggacgttg gccaggagat cgtgatcgac caggttctgg
     1381 cagtcggcaa cggcgcagaa ctgaaggtgg gcacgcccct ggtgtccggc gcggcggtga
     1441 aggccacgat cgtggcccat ggcaagcacg acaaggtgcg cattttcaag atgcgccgtc
     1501 gcaagcacta tcagaagcgc caaggccatc gccagcagtt caccgagctg caaatcggtg
     1561 caatcactgc ctaaggaagg acaagagtca tggcacagaa aaaaggcggc ggctctacgc
     1621 gaaacgggcg cgactccaag cccaagatgc tcggcgtgaa ggcttacggt ggtgagctga
     1681 tcagcgcggg ctccatcatc gtgcgtcagc gcggcacgcg catccacccc ggcgtgaacg
     1741 tcggcgtggg caaggatcac acgctgtttg ccctggtgga cggtcatgtg tcctttggca
     1801 ccaagggtgc cctgtccaag cacacggtca gcgtgacgcc ggcctaaggc ctggcctgtc
     1861 agttgctgac gtcatgacaa agccccgatt cgtcggggct ttgttgtttt caacgcggaa
     1921 ccgcctggcc atatggcgtc cgccgatggc acccatggcc caaagggcag cgcgggtgcg
     1981 gttttgtaaa ctggataccc atgaagttcg tcgacgaagc ctttatcgac attgccgctg
     2041 gtgatggtgg caatggctgc gtgtcattcc gacacgaaaa gtacaaggag ttcggtgggc
     2101 ccgatggcgg cgatggcggg cgcggtggcc atgtgtttgc cgttgccgac ccgaatctca
     2161 ataccctggt ggactttcgc ttctcgcgcc gccatgacgc caagcgcggc gagcatggca
     2221 agggatcgga catgttcggt gcagcgggta gcgacattac gctgaagatg ccggtgggca
     2281 ccatcatcag cgatgccaac acgggtgagc agctgtacga gctgctcacg ccgggcgagg
     2341 tgatcaccat tgccaagggc ggcgatggtg gctttggcaa cctgcgtttc aagagcgcca
     2401 tcaaccgggc gccgcgccag aagacaccgg gctggcccgg cgagcgcaaa aacttgaagc
     2461 tggagctgaa agtgctggcc gatgtcggat tgctgggcat gcccaatgcc ggcaagtcca
     2521 ccttcatcgc cgcggtctcc aatgccaggc caaaaattgc cgactacccg ttcacgacgc
     2581 tctacccgaa tcttggcgtg gtgcgcgtgg ggccggagca gagtttcgtg gttgccgata
     2641 tccctggcct gatcgagggt gcatccgagg gcgctgggct gggccaccag tttctgcgcc
     2701 acctgcagcg cacgcgcttg ctgctgcatg tggtggacat ggcgcctttc gatgatgccg
     2761 tcgatcccgt ggcccaggcc aaggccatcg tcgccgagct caagaaatac gacgaagggc
     2821 tgcatggcaa gccgcgctgg ctggtgctca acaagctgga catggtgccg gccgaggaac
     2881 gtgccgtgcg ggtcaaggac ttcgtcaagc gcttcaagtg gaaggggccg gtgtacgaga
     2941 tttctgccct gacacgcgag ggttgcgacc cgctggtgaa ggcgatcttc cagcatgtgc
     3001 atgccgagca actggcgcaa aatgcccccg ccgaggtcga tccacgtttt gccggcgagc
     3061 atggccagga ttgatcatct attcactata aaatatatag ctgtttgcgt ttgatagatg
     3121 ggcgcttgag caatatttca atctaaacat ggtttccagt gtcttgcgcg acgcgcgtcg
     3181 catcgtggtc aaagtgggct ccagccttgt caccaatgag gggcgcggcc tggacgaggt
     3241 cgccattggc gagtggagcc gtcagctggc ggcgctggtg cgcggtgagg gcggcgtggc
     3301 gcgcgaggtc atcatggtct ccagcggcgc catcgccgag ggcatgaagc gcctgggctg
     3361 gtccagccgc ccaagcgaaa tccatgagct gcaggcggct gccgccgtag ggcagatggg
     3421 cctggcgcag atgtatgaga ccaagctgcg cgagcaggcc atgggcagcg cccaggtgct
     3481 gctgacccat gccgacctgg ccgaccgtga gcgttacctg aatgcgcgct ccacgttgct
     3541 gacgctgctg gagctgggcg tggtgccggt gatcaacgaa aacgacaccg tggtcaccga
     3601 cgagatcaag tttggcgaca acgacaccct gggcgcgctg gtggccaatc tggtggaggc
     3661 tgatgtcctg gtgatcctga ccgaccagaa gggcctatac accgccgacc cgcgccgcga
     3721 cccgcaggcg caattcgtgc atgaggccaa agccggcgat ccggcgctgg aggtcatggc
     3781 cggtggcgcg ggctccagca ttggcaaggg cggcatgatc acgaagattg tggcggccaa
     3841 gcgcgccgca ggttccggcg cttccaccgt catcgcctgg gggcgcgagc cggacgtgct
     3901 gctgcgcctg gcgcgtggcg aggccatcgg cacgctgctg gtggcgcaga cgcagaaaaa
     3961 acaggcgcgc aagcgctgga tggtggatca cctgcaactg cgcggatcgg tgaccgtgga
     4021 cgcgggcgcg gccgccaagc tgcgcggcga gggcaagagc ctgctgccca taggcatggt
     4081 ggcggtggag ggtgatttcg cgcgtggcga tgtgatcgcg gtgcgcgatg cgggcggtac
     4141 cgagattgcc cgcggtctgg ccaactatgc cagcgccgag gcgcgtcggc tgtgccgcaa
     4201 gccgtcctcg gagttcgagc gcctgctggg ttatgcggcc gagcccgaaa tggtgcaccg
     4261 cgacaacatg gtggtgttta cggcctgacc agcggccggc cgccgcgccg ccatgaacgt
     4321 gccaactacc tgctggccgg cttgttgtac tggtcggcgg gcgctgcctc ctgcagtggc
     4381 gccggcatgc tgttctgtgg gtcgggatcg aagctggccc cgggtggcag ctccatcccc
     4441 ggtttgagca tcatcgagac ggagcgatgc atggccggct ggccaccctc ggcttcgcgt
     4501 gcacgcatgc cgctgcgcag gtagcggttg cgctgttcaa aacgcggcag aaagcgcgcc
     4561 agctcggtca gcgccatttc gtagacgcca cgcttgaact cgaccaccac gtccagtggc
     4621 acccagtagt cgttccagcg ccaggcatcg aactcggggt ggtcggtggc gcgcaggttc
     4681 aagtcccagt catggcccag caactgcagc aggtaccaga tctgtttctg tccacggtag
     4741 tggccgcgcg cgtcgcggcg gatgtaccgg tcaggcacct cgtagcgcaa ccaatcccgc
     4801 gtacgggcaa ttacacgtac ctggctgggc tgcagaccta cctcttcgtg cagttcacgg
     4861 aacatggctt gttccgggtt ttctccgcgg tcaatgccgc cctgagggaa ctgccagctg
     4921 tgggtgcgta tgcgcttgcc ccagaatacc tggtttctct ggttgagcag gatgatgccg
     4981 acgtttgggc gaaagccgtc cctgtcaagc ataatctaac cccaaatttt taaaactgtg
     5041 gtcattatgc acgctgcccc ggctgcggca aacctagaag ccccagttgc cgagcgcgtt
     5101 cgatgaaagc ttcccaattc ttcatttcca ccctgaaaga ggcaccggcc gacgccgaag
     5161 ttgccagcca taaactcatg atgcgcgccg gcctgatcaa gaagctgggg gccggcatct
     5221 acaactacat gcccatgggc ctgcgcgtga tccgcaaggt cgaatcgata gtgcgcgagg
     5281 agatgaaccg cgccggcgcg gtcgaatgca ccatgcccgt ggtgcagccg gccgagttgt
     5341 ggcaggagac ggggcgcttt gacaagatgg ggccggagtt gctgcgcata caggatcgtc
     5401 atgggcgcga cttcatcatc cagcccacca gcgaagaagt ggtcactgac atcgcgcgcc
     5461 aggaactgcg cagctacaag caactgccca agaacctcta ccagatacaa accaagttcc
     5521 gcgacgagcg ccgtccgcgc tttggcctga tgcgcgggcg cgagttcatc atgaaggacg
     5581 cctacagctt cgaccgtgac caggcggcgg ccaaggccag ctaccaggtc atggcccagg
     5641 cctatcggcg catcttcgat cgcttcggtc tgacctaccg cgccgtggcg gccgattcgg
     5701 gcgccattgg cggcgacctg agcgaggagt tccaggtcat cgccgccacc ggcgaggacg
     5761 ccatcgtcta ctgcccgggc agcgactacg ccgcgaacat ggaaaaggcc gaggccctgg
     5821 cgccagctgg cccgcgcctg gcagctgcag cgcccatgac gcgcacgcct acgcccggca
     5881 aggccacctg cgcccaggtg gccgagctgc tgaacgtgcc gctggcaacc accgtcaagt
     5941 cgctggtgct ggccaccgac gagaccaacg aggcgggcga agtcgtccaa tcccaggtct
     6001 ggctgctgct cttgcgtggc gaccatgaca tgaacgagat caaggtctcc aaggtctccg
     6061 gcctggacaa gggcttccgt ttcgccaccg tggccgagat cgaggcgcat ttcggcgcca
     6121 agcccggcta cctgggtccg atcggcctca agcaatcggt gcggatcgtt gccgaccgcg
     6181 aggtggccgt gatggccgac tggatctgcg gcgccaacga ggaggactac cacatcaccg
     6241 gcgtgaactg gggccgcgac ctgcccgagc ccgatgctgt ggccgacctg cgcaacgtgg
     6301 tggcgggcga cccgtccccc gatggcaagg gcgagctcgc catagagcgt ggcatcgagg
     6361 tgggccatgt gttctacctg ggcaccaagt attccagggc catggatgcc acctttctgg
     6421 gcgaggatgg caagccggcc ttcttcgaga tgggttgtta cggcataggc atcacacgcc
     6481 tgcccgcggc cgccatcgag caaaaccatg acgagcgcgg catcatctgg cccgatgcca
     6541 ttgccccctt caccgtggtc atctgcccga tcggcatgga tcgcagtcaa gaagtccggg
     6601 cggcagccga aaagctgtat ggcgagctgc tcgccgccgg ggtggatgtg attctggacg
     6661 accggggcga gcgcccgggc gccatgtttg ccgattggga gctgatcggc gtgccgcacc
     6721 gtatcaccat cggcgacaag ggtctcaagg agggccaggt ggaataccag catcgccgtg
     6781 acagcgccgc taccaagctc ggggtggcgg aggtgtttgc ttacgtgaag gaacgtgtga
     6841 aggtatgacc agagggggag attcagcttc gggcttgtcg cggcgcgcct gtctggcatc
     6901 gctggcggtc ggcccgagtg cttggctggg cctgccggct gctgcgcatg ccggtggcca
     6961 gctggaggag ccgttgatgg actcggtgcg catggcgctc agttccgccg tggccgacct
     7021 ggcgccacca gagccgctgt ttgtcacgac cgagtcgcgt ctgacctacc tgcgctggct
     7081 gggcaccatg agcgaccgcc tgcgccgcag caaacccgac tgggaggtgc gacgcgactt
     7141 cctgcagacc gtctggtacg agtccaagcg tgccgggctg gatgtgtccc tggtcatggg
     7201 cctgatccag gtggagagcg cgtttcgcaa gtttgccgtc tccagcgtgg gcgcgcgtgg
     7261 ctatatgcag gtcatgccgt tctggacccg tgcgatcggc gatggcgacg cgggcaagct
     7321 gtttcagatg cagaccaatc tgcgctttgg ctgcgtcatc ctgcgccact acctggatcg
     7381 cgagcgcggc gacctgttca tggccctggg gcgttacaac ggcagccgtg gcaagccgcc
     7441 gtatccgaac gcggtgttcg cggcgcaaag ccgctgggcc tttcaggatc gcgctgcctc
     7501 ggcctgagcc ccgttctccg ctggcgccgt gaggagccgc gagaccatga cctggtcgat
     7561 gcggtagctg tccacgtcca atacctcgaa cttgtagtgg ccaacggtca cgctgtcggt
     7621 gcgccgaggc acacggcgca gcatcaccat cagaaaaccc gccaaggttt cgtagtcctc
     7681 ggaatgcggc agctcgtcca ggccgaggac atgcagcacg tcttcgatgg gggccgtacc
     7741 gtcgatgagc caggagtttt cgtcgcggcg cacgatctgc tcctcgtccg cggggccgac
     7801 caggtcgccc atcaccgtgc tcatcacgtc gttcagcgtg accacgccca ccacccggct
     7861 gtattcgttg acgatgacgg cgaagtcctc gtgcacctgg cggaaatgtg ccagcacctc
     7921 ggccagcgac agccggtcgg gcacgatgag caccttgcgc accagctggt cgtcggccag
     7981 cgagatgggc tggttgttca gcacgcgctg gaacaaatcc tttgcatcca catagcccac
     8041 cacatggtcg atgtcgccat cgcacaccgg gtaggtcgag aacggctcgg ccgcgatgcg
     8101 cgcgcggatc agctcatcgg ggtcgtcgcg caggaagaag gcaatgcgct cgcgcagcgt
     8161 catggccgag gtcacgctgc gggtgtcgag ctcgaagaca ttggcgatca cctgctgctc
     8221 gggcagggcc agcacgccgg cgcgtgcgcc ggcctcggtc atggccagga tgtcttcggg
     8281 cgtgatgcgc tcgtcacgct ggctggacag gcccatcagg cgcagcaggg catcggcgca
     8341 gcggctgtag agccagacta tgggtttgaa cagcgccatg cagccctgca tggggcgcac
     8401 cacgcgcagc gccacgacct ccgggtgggc catgcccagg cgttttggca gcagatcggc
     8461 aaacaggatg aacagcgagg tcacggccag gaaggagccg acaaaaccca gcgtctgggc
     8521 cgacttctca ggcagccaca gcgcaaacag ggcgttgaaa taggggctga gcgccccttc
     8581 gccgatgatg ccgcccagga tggcgacggc gttgagcccg atctgcacca cggtgaaata
     8641 ctcgccgggc tggtcctgca tcaacagcac acgatcggcg cgcgtgtcgc cttcgtccgc
     8701 catctggcgc aggcgcaggc ggcgcgatgc ggcgagcgaa atttctgcca ccgagaaaaa
     8761 cgcgctggcg gcaatcaaca gggcaatgag cagcagactg tgggtcaggg acagattcat
     8821 gggcaggcgc cgcggcgtgc gctgggcgca ccatggcagc cagtgtagcc cgcgcctact
     8881 gcaggaaacc gatgcggctc ttgccaccgg ccgtgcgcgg caggtcggcc acctcgacgc
     8941 gctcgcgctg ctccaggcgg gcgttgccaa aaccggtcat cagggcgcgg cgcatctcgc
     9001 gcggcggcat ctgggccagc aggtccagca catcggcctg ggcctcgggc tcgaacaggc
     9061 ggccccagtc atgctcggcg cgtatgccct ggtacagccg ctgggcgatg gcgcgtgcct
     9121 gcgccagcgt gggcgcctgc accgcgaaga cgttcatgcg gttcaggatg gggtcgggta
     9181 tggcgcgctc gtcgttcgcc gtggtgatcc agatgacctg gctggcgtct atggccacgt
     9241 cggcgaactc gtcggtgaag ctctgcgcgg tgtcgtgctc caggagggcg tagagggcgc
     9301 ccagcgggtc gtactgcgcg tcgccggcgg ccttgtcgat ctcgtccacc acgatcaccg
     9361 gattggcata ttcgccttcc accagggcct cgaagacctt gcccgggcgc gcgcccttcc
     9421 actgcgctga cgagcccgag agtagccagc cggcggtcat ggaactcatc ggcaccaggt
     9481 tcatgcccgt gcccagcagg gctgcgagct ggcgtgcgaa atgcgttttg cctatgcccg
     9541 gggcgcccag cagcagcatg ggcgtgacct ccaggccatc gctgctgccc tgggccaggg
     9601 ccacctggcg gcgcacgtcg tcgagcactt cggtgaagtt gggcagctgc tcgtacaggg
     9661 ccgccatgtc gggcaggccg gagggtttga cctggaagcg ctcggggccg cgctcgagca
     9721 tgcgttcgta caccgtgcgc aacggttcat ggtcgcgctg cgcgccggca tcctgcagcc
     9781 gggccagctt gcgcgccacg tcggccgggt ggaacacgct gcgcatctgg gccaccggca
     9841 ggctcagggc aggggcttgc ggtacaaggc agtggctggt catctcatgc tctccccagg
     9901 gcaatggaca cagccattca agcacagcgg ggcaacggcg gttgtcggag taatcccgca
     9961 tcgccggcgc gggcacaaaa aaaaccgccc tcgcgagggc ggttcttgcg cggcggaggg
    10021 caggtcagcc ctgggccttg gtgcccagca cctgctgcag ctcgccagac tcgtacatct
    10081 ccatcatgat gtccgagcca ccgatgaatt cacctttgac atacagctgc gggatggtgg
    10141 gccagtggct gaagtccttg atgccctggc ggatctcgtc gtcctcgagc acgttcacgg
    10201 ccgcgatgtc cttgggatcg acgccgcagg ccttgaggat ctgcacggcg cggcccgaga
    10261 agccgcacat gggaaagctg gcattgccct tcatgaacag cagcacgtcg ttggtcttga
    10321 ccagttgctc aatgcgttgc ttcacgtcgc tcatcaaatt ctcctgaagt ggctgccccg
    10381 gtgtggggcg ataaccggcg attatttcac cgaaggcgat agccctactg gcattgaccc
    10441 gccgctgcag cgggctatgc cggccaggtc gttacggccc tgcacctggg caaggcccgc
    10501 ggcacgcagc agggcctgca cggctggcgc ctgatcccag ccatgctcga tcagcagcca
    10561 gccaccgggg gccagcgccc tgggggcctg ggcgatgatg gcgcggatgt cggccaggcc
    10621 gtcggcgccg ctggccaggg ccgacagcgg ctcgtgcgtg agggctgcca gatgcgggtc
    10681 ctgcgccggg atgtagggcg ggttgctgac gatggcgtca aacgggccgg tgatgctggc
    10741 cagccagttg ccatgctgaa ggcgcaccgg caggcccagg cgctcggcat tggcgcgcgc
    10801 cacggccagg gcgtctgcgc tggcgtccac ggccagcacg ctggcgtcgc ggcgctcatg
    10861 ctgcagcgcc agggcaatgg cgccgctgcc cgtgcccagg tcggcgatgc gcggggcatg
    10921 ctggttttgc aggacctcca gcgcccaggc caccagggtc tcggtgtcgg ggcgcgggtc
    10981 gagcacgcgc gcgtccacgg ccaggggcag gccccagaat tccttttggc ccgtcaggta
    11041 ggccacgggc tcgccgcccg cgcggcgcgc gcacagcgcg gtgtagcgct gtccatcgtg
    11101 cggcgccagg gcgtcgccgt catgcgtgag cagccaggcg cggtcgttca cgggccggcc
    11161 cagcagatgc agcagcagca tctgcgcatc gatgcgcgcc aggccctggg cctgtgcccg
    11221 ggccaatgcc tgggcgatgg tggtggtggg catgaatgct ttgttattga tagctgctgg
    11281 cgcttgatgg ataagcgtac aaggccaaaa tcaatccagg cccagttcgg ccagctgctc
    11341 ggcctcgcgg gcatgctgca gggcctgcaa cacctcgtcc agatcgccct ccatgatggt
    11401 cagcagcttg tacagcgtga ggttgatgcg gtggtcggtc aggcgcccct gcgggaagtt
    11461 gtaggtgcgg atgcggtccg agcggtcgcc gctgccgatc aggcccttgc gcgtggctgc
    11521 ttccttggcg gcgcgttcgc tgcgctcctt ctcctggatg cgcgcctgca gcacctgcaa
    11581 ggccttggcc ttgttgctgt gctggctgcg tccgtcctgg cattcggcga cgatgcctgt
    11641 ggggatatgc accacgcgca cggccgaatc cgtcttgttg atgtgctggc cgccggcgcc
    11701 actggcgcgg aaggtgtcta tgcgcaggtc ggccgggttc agcgtgatag cggcggccgc
    11761 gtcgggctcg ggcatcacgg ccacggtgca ggcgctggtg tggatgcgcc cctgggtctc
    11821 ggtatccggc acgcgctgca cgcggtggcc gcccgattca aagcgcagcg cgccgtaggc
    11881 gttgtcgccg tccacgcgca gcaccacctc cttgtagccg ccgagctcgc tttcgttggc
    11941 gctcaggacc tcgaccttcc agcccacgtt ggcggcgtgg cgcgtgtaca tgcgcgccag
    12001 gtcggcggca aacagggccg attcgtcgcc gccggtgccg gcgcggattt caacaaaggc
    12061 cgcgcgcgcg tcgtcggggt ctttgggcag cagcaggcgc tgcagctcgt cttcgagtgc
    12121 caccagctcg tcttgcgcgc cctggatttc ttcctgtgcc atctcggcca tgtcggggtc
    12181 ggccagcagc tcctgggcgg cggccaggtc ggcctcgcgc tgctggtagc gggcgtagcg
    12241 cccggcgatc tgcgtgacct cggcgtgctc gcgcgagatg gtgcggtatt gcgccatgtc
    12301 ggccatgatg tcttcgcgcg agagcaggaa gtcaagctct tgcaggcgct gcgcgtagcg
    12361 ctccaactgg ctgcggagaa agggtttcat cggggaaagg aaaatttatt gctactgttt
    12421 atgtagctat tagcgcttgc ttggtaagcg ctggatgccg atttggcttg tggtgcagcg
    12481 gcgctacagc ccggtcttgc tttgcgagcg caggaacagg cgagaaaccg cctcggccgt
    12541 ctgggcgcgc gcatcggcgt cgccggcgcg caactcggcc atggtgccgt gcagcatctt
    12601 ttgcgcgagg ccacgcgaca gggcttcgag caccacgtcg atgtcctcgc ccttggccag
    12661 caggcgcttg gcgcgtgcga tctccagggc gcgccactca tcggcctggc tgttgagctg
    12721 gcggatcagc ggcaccacgg cgccggcctg gctggcgggg ctgcgcagct ccatccagtg
    12781 catgaagttc tgcaccccgg catcgatgat ggcctcggcc tgggccacgg ccgcctggcg
    12841 gttggcctgg gcggtctgca ccacgccggc caggtcgtcc acggtgtaca ggtagacgtc
    12901 ctcgagctgt ttgacctcgg gctcgatgtc gcgcggcacg gccagatcga ccatgaagat
    12961 ggggcggtgg cggcgtttct tcagggcgcg ctcgacggcg cccagtccga tgatgggcag
    13021 gctgctggcc gtgcagctga tgacggcgtc gtattcgtgc aggtgctcgg gcagatcggc
    13081 caggcgcatc acatcgccgc caaagcgcgt ggcgagcttc tcgccgcgct ccagcgtgcg
    13141 gttggctatt gatatctgct tcgggttctt ggccgcgaag tgcgtggcgc acagctcgat
    13201 catctcgccc gcaccgacga acagcacgcg gatcttggac aggtcttcga acagctggcc
    13261 cgccaggcgc acggcggcgg cggccatgct gatgctgtgc gcgccgatgt cggtgctggt
    13321 gcgcacctcc ttggccacgg caaagctgcg ctgaaagagt tggttgagcg tggtgcccag
    13381 ggcgcccgcg ccttccgccg cgcgcacggc atccttcatc tggcccagga tctgggcctc
    13441 gcccagcacc atggagtcga gcccgctggc cacgcggaag gcgtggcgcg ccaccaggcc
    13501 gctctccagc gtataggagt gcgagcgcag cagcgaaggg ctgacaccgc cgctggcggc
    13561 cagccagtcc agcgtgtgat caagcgccgg cttgtgcgca gcacagtaga tctcggtgcg
    13621 gttgcaggtg gagatgatgg ccgtctccac acccgggtgg ctgctggcac cgctggccag
    13681 cgactggcgc aggccgtgca gcgtgggcgc gatctgatcg atcgcgaacg cgaagcggcc
    13741 ccgcagatcc agcggtgcgg tgtggtggtt gatgccgagt gcccaaactg ccatatccgg
    13801 ggattataaa atcgcccgcg caatcaggca cgctaccggt caaatgcttg atttacatca
    13861 tgtttgcatg agcccgctcg ccatcctgaa ccaccttctg aactttgccg caccggccct
    13921 ggcgctggcg gtgttgctcg tgctggtcag ccatttattc atgcgaaaaa cggctgcagc
    13981 acatggctgg gttgcgccta taactatcaa ttttgttgtt ggctgcgcgg tgctggtggc
    14041 cggcctggcg tggctggggc gcgatggccg gatgctcacc tacgccgccc tggtgctggc
    14101 ctgcgccagc agccagtggc tcatgttgcg cgcctggcgc ggctgaagtc cgattagatc
    14161 cgctctatca gcgagcgccc gggtgccgcc tcgctcacgg gctgggccac ggtggtggtg
    14221 gcgggtggcg acagcgtcag gccggcctcg cgcagcgcgt ccaggatgga gaacagcaca
    14281 tcgctgcgca cgcccgagac ctggcgcggg ctgctgacgt agccaatggc cagaaaggtc
    14341 agcgtgccgc cctgtatgcc ctccagcgtg accgagggcg ccgggtcgtc cagcatggcc
    14401 tcctgcgcct caaaggcctg caggatcagc gcgcgcatgc gccgcgcgtc ggtgtccagt
    14461 ggtgccggca ggcgcagcag cacgcggccc tgggcgctgt ccagggtcac gttgcgcacg
    14521 gtcttggtga tgaactccga gttcggcacg atcaccgtgg agcggtcgct ttgctggatc
    14581 tcggtggcgc gcacgttcac acggcgcaca tcgccctcgg tatcgcccag caccacccag
    14641 tcgcccacct tcaccgggcg ctcggccagc aggatcaggc cggagatgaa gttctgcacg
    14701 atggcctgca ggccaaagcc tatgcccacg gtgagcgcgc tggccaccca ggcgatgcgc
    14761 tccacgctga tccccaggcc cgccagcgcc accgccacca ccaccaccgc gccggcatag
    14821 cccagcagcg tgacgatgga ggtgcgcatg cccggctcca gcgtggtgtg cggaaagaag
    14881 cgctcgctca gccaatgctt gagcaggcgt atggccagaa agccggcgca ggccacggcc
    14941 agtgccgtga gcagggcctg gggcgccagc gtgatgtcgc caatgcgcag gctgtgatcg
    15001 acgctgctgc cgcgccggaa cagctcgtcc ggatccgtgc ccagcggcgc catcagcgcc
    15061 accaccatgt agaaaaacag caccagccgc agcacgcccg agaccagcac cgccgcctga
    15121 tccagcagcc tggcgtccag ccccagggtg ccgtggatgc gcgcgccaaa gccgcccttg
    15181 gacgacagca gcgtgtcgca caggtcgtcg cccagctgca tcagcaggta gctggtggca
    15241 aacaccacgc cagaccagac catctgccgt gccagcatgc cggccagggc cacatagccc
    15301 agggccacgg ccagcacggc cacgcccacg gccaggccgg cggccgcgag gatcaggccc
    15361 agccacagcg ggcgctcgcc ctggccatgc ggcgcgccgg catcggcatc cgacgcggct
    15421 ggcgcggcgc gcatatggcc cagtgccttg cccaccgtca gcgacagcag cagcgcaaac
    15481 agggcgtgca ccaagacctc ggccgacagg ctggcgccga cgatgacgtt gagctcggcc
    15541 accagcgccc ccagggccga caggctggca atccaccagg gaaaggggct caggcgtcgc
    15601 gccagctcgt cggcgatagg cagcagccgc cacgagctgc gccggcgcga gagcagggca
    15661 tggcccagtg cgatcacgaa gctggcgtag accacggcgc gcaggctggc ctgctgcaag
    15721 gcgcgcagat cgtcgcccag cggcgcgccc agctgcagcg cgttccaggc cagctgcacg
    15781 gccgtgccaa cgatgaacac atgggtcacg atggcggcgc tggccagcag ggtgcgccgc
    15841 agccgtgccg cgggcaggcg cggcggcagg gcgcgcagca aaatgcgctc ggccagccag
    15901 gggccgccca cggccagcag cagcgccagt gccaggtagg cccagaaggc gttgtggcgc
    15961 ggcgactgca gcgtggccct gacggcgccg gccagatccg cgcccagggc ctgcaggcgc
    16021 accagatcgc gcggcgcggc attgcgcaga ttgcgccaga acgtgggggc cagcggcgag
    16081 tcgctgcgcg cggtcaaggc ggcacggaat tgcgcgcgcc gctggcgcac caggtcggcg
    16141 ctgcgctgtt cggcatcgac ggcgaccagg cgcgccagct tgagctcgga gtcgatggcc
    16201 gagcgctgct gggtcagcag cttgcgctgt tgcgccacgt ccggcgcatc ggcgggcgcg
    16261 ccgctgcccg gggccgggcc caggccttcg aggcggcggt cgatttcggc cagctcggtg
    16321 ccgcggtggc tgaccacgcg ctctgccgct tggccgatgg cgttgatgtc ggccagcagc
    16381 ttgcgcccat cgtcgggctc gccgagcttg agcggaatgg cgtccagctg cttgcgcagc
    16441 gcatccacct ggggcagggg ggtgccgttg tcgccgggct tgtcggccgc ggcggtgggt
    16501 gctgcgtcgc tgctggcggg ccggccctgg gccaatgtgg gcggtgtgcc cagggccatg
    16561 agccaggcga cggacagcca gcacagcagc cgcgccaggg gtcggggcgc gtgggccatc
    16621 tgcgtgaggt ctgggcgggg gggatggctg cggttcatat gggccgcatt gtctgccgtc
    16681 cgcctggtgc ttcaggttgc ggcggggctg aacaagtcca cgcggtcggt gatgatgccg
    16741 tcggtgttca gggcgatcag gcgctcggcg gcccattcgt cgttgacggt gtagctcagg
    16801 gcgcgcaggc ccgcgccatg cacgcgtgcc accagggcgg cgtcccacag tgcgtgattc
    16861 agcaccatgg cctggcagcc caggtcgagg gcggtctgca catcggctgc gccatcggcc
    16921 agcttgtcca gcagcaggcc gcgcggcagc gcgggcgccg tctcgcgtgc gcctgtcagg
    16981 gccgcgggtt taaaggacgt gagcagcggc ggaacggccg tggccggcca gatgcgcgag
    17041 agcagttcgc ccacggcgcg gcccgtggcc agctcctgcc cgggcgtggg cttgatctcc
    17101 acgttcaggt ggtggccatt ggccaggcag tagcgcgcca gcgcctccag cgtgggcatg
    17161 gcctcgccgg cataggcgcg cgaatgccag ccgcccgcgt ccagctgcgc cagctcgccc
    17221 atgctgcgct cgccggccgc gccgtggccg ttggtggtgc gctccagcgt ggcgtcgtgc
    17281 agcagaaaca gcacgccatc gctgctgagc ttggcgtcgc actcgaacat gcggtagcca
    17341 tgccgggcgc ccaggcggaa ggcggccagg gtgttctcgg gcgccagctt gccggcgcca
    17401 cggtgggcga tccagcgcgg gtagggccag ggtggcagtg gcgtactcat tcagatcctc
    17461 ttgccggttt cctggctgaa ccagtgcagg cgatcctcgc gcgggacgat atgcgccgtc
    17521 tcgccggggc gggggtaggc gctgccttcc tccacgcgca cggtcacctc ctcgccgccc
    17581 accttgccgt agagcaggcg ctcggcaccc agcagctcca cggtctcgac ctggaactgc
    17641 cagccgccgc tggccgccag gtcgatatgt tcggggcgta tgcccaggat gcgcccggcc
    17701 tggccgccgg gggcgttctt cagcagattc atgggcggcg agcctatgaa gccggcgacg
    17761 aaggtggagg caggctggtg atagacctcc tcgggcgtgc cgaactgctc cacgttgccg
    17821 gcgttcatga cgatcatgcg ctgcgccagg gtcatggcct cgacctggtc gtgcgtcacg
    17881 aacaggctgg tgatgccgag ttcggcgtgc agcttctgga tctcgatgcg cgtctggccg
    17941 cgcagcttgg cgtccaggtt ggacagcggc tcgtcgaaca gaaacacctg cggctggcgc
    18001 acgatggcgc ggcccatggc cacgcgctgg cgctggccgc ccgacagctc gcgcggcttg
    18061 cgctccagga ggtgcgacag ttccagaatc ttggccgcct tgtccacgcg ctggcgtatc
    18121 tcgtcggccg gcaccttggc gagcttcagg ccataggcca tgttctcgta gttgctcata
    18181 tgcgggtaga gcgcgtagtt ctggaacacc atggcgatgt tgcgcttggc cggctccagg
    18241 ctgttgaccc gcttgtcgcc gatgaagagg tcgccgccgg tgatctcctc cagccccgcg
    18301 atcatgcgca gcagcgtgga cttgccgcag cccgacgggc cgaccagcac gatgaattct
    18361 ccgtcctgga tctcggcgtt cacgccgtgg atgacgggca cggcagactt gccgctgcca
    18421 tagcgcttga cgatgttttt cagggagatg gaggccatgt gagtgcgagt cgtaaggcgt
    18481 tgggcgcggt gaggccttcg agcaggccgg cgatggagat gtttttgtcg agccggggcc
    18541 agtggatgcc ctcgcccatg cccagccatt caaaggccgc caactgctcg gcagtggccc
    18601 gaatccagcg ggggaaccag accaggggca cggacaggat atggccgcag tgcagtgtca
    18661 tcaccagcgc gtcggcggcg cattgcaggg ccacggcatg gggtgcggtt tcaagtgcca
    18721 aagtactcat tccaggtctt cgtgaatttt ggttgacgtt tctcttgtac tgaattggtg
    18781 aaatttttga aatcgtgcgt gaatatattc ggcgtaatac aaatttacgt cttgaatttg
    18841 agggttgtgc cttgcaataa attggaggtg aaaaatccag gccggtttgg cactagtttg
    18901 aatcatcaaa aaagaattct attgaatctg cgttggtgct gggaaagatc cgttttccga
    18961 agcgaacaat acgctttgag gtggtcggca aaggattgtc gtattcccgc gatgcgtact
    19021 cggccgcgta gcccatcagc agaagagtgg tcggaaatat ctggctgtgc gatcggattt
    19081 cgccgtcatt tctctgaaat tggtttctaa gatctgggtc ggtaaataga ataagtggca
    19141 cggacaactc ctccggatga ggtttgttgt tgcaatgggg aataacgcca tttcctaggt
    19201 gctggccatg atctgatgtg taaaaaatca ccacattgcg catgttaacg ccgtctagta
    19261 gttcattgag gaaatttcct tttgaatacc tcaatgcctt tcggtattgc tcttcaatcg
    19321 gtaagctccg gtctagattt ccatccgggt agttggagaa gtactgatag tgcgccccct
    19381 ttagaacaat gtacgcaaag tgccgtccat cggccttgag cagtccgttc agccggcggg
    19441 caatctttag gtctgagtcc agtcctgcgg ccatgggcac cacctcgtca atcagccttt
    19501 tctcaggcgg ccagagatag ttttgtggat tcccatgtac ctgcccatcc atcaggatcg
    19561 tccgaaatcc cgcctgcttg gcatatgccc acaccgtact gttggttctg aggtctgtcg
    19621 aggcattcag cttgcttaca ttcacgcccc agcgcaaggc ggcattgctt tgggcgctgc
    19681 agttacctaa ggagcgcgcc tcgccaaagt ctatcccacc ccacctggac acccactccg
    19741 atcggaacat ttcatcatag tatcgccacg aaatactctc atcaattaac cagatgattt
    19801 tgttcgcgga tggcttgaaa tccggtttta cagatgccgg cattctattt ggaatttcct
    19861 cagtcatggt ctgatatgcc atgccgtaga cattcatttc ggcgccgcga tctctgccca
    19921 atgcttttag tgctggatca gccgccagaa atatcatgag cactactatt gtcaagatcg
    19981 gggcattttc tggatgtggc gccatcttgc taaatactgc gtattttatt gcctttctgg
    20041 taagcgtgaa aagaccaact gcaatgcccg ctctggttac gccccaaata aaatccttat
    20101 aaaattcctt gagggcggaa agggattgtc gtgattccgc tagcatccat tgaaatgttg
    20161 ataggttaat caggtcgtac aggatgcttt gataaaccag attaacggtg ccggaaataa
    20221 atacgagcag cagcacactc cgaaatgccc agcctgagat gcttccaatg acaagaaata
    20281 acaacagacc ttggctggca taggagacaa gattccaaat cgcattgaac tgatcatccg
    20341 cggcccatcg gtacaggacg ccgaccacga gaattcgata attgagtgtt agataccccg
    20401 tggcaaacag cagcagggca acgcctatcc atctagcctt gatccatggt ttttttttgt
    20461 taatcatttc aatgcggttt tttctggctg tgatgttatg tgtgggtgcg ttttttttgt
    20521 tgaaatagaa aagaagcggt aggcgccaat atgcaacacg gtgggcgtgg ggcttatttt
    20581 tctgtatcca ccaggccctt gacgaaccat ttctgcatca ggatcaccac cagcgtcggc
    20641 ggcagcatgg ccagtatggc ggtggccatg acgatgttcc agtccacggc ggcctcgccg
    20701 ccggcgagca tgcgcttgat gcctatcacc acggggtaca tgtcctcgct ggtggtcatg
    20761 agcagcggcc acaggtactg gttccagccg tagatgaact ggatcacgaa cagcgc
//
LOCUS       DAOMTQ010000038        20694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_304_length_20694_cov_29.788895, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000038 DAOMTQ010000000
VERSION     DAOMTQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 20694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20694
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_20694_cov_29.788895"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK518_05690"
     CDS             complement(<1..536)
                     /locus_tag="PK518_05690"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRM48439.1"
                     /translation="MPRFYCPLPLAAGAQVTLPPQAARHVQVLRLQPGGVITLFNGEG
                     GEWTASVTRMGRSDVDVEIGAHTAVEREAARAVHLVVGMPANERMDWLVEKATELGAA
                     SIQPVTTARSVVKLAGERAAKRQAHWQAIAVAACEQCGRNRVPPVHAPLALADWLRGG
                     QSEGTRLLLSLRDGSRPLR"
     gene            complement(834..1937)
                     /locus_tag="PK518_05695"
     CDS             complement(834..1937)
                     /locus_tag="PK518_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRM48440.1"
                     /translation="MSHPLPPLPSGASAVQWNDPARQSAFLAWLAPIAAAQNLLPASL
                     RPASADASFRRYLRLDAQDGSSRIVMDAPPDKEDCRPFVHVQGLMQSAGLPVPQIHAW
                     DQTHGFMLLSDLGVQTAIERLDEAQPAAAHAWYLQAVDLLIDWQKASRPGVLPAYDDA
                     LLRRELQLFPDWYIARHRQVTLTDAQQATLAKAFETIVAQNLAAPSVYVHRDFMMRNL
                     MVRADGRLSVLDFQDAVFGPVTYDIASLMRDAFISWEEDFVIDITVRYWEKARKAGLI
                     GAASASGWGADFGEFYRAVDFMALQRHLKVAGIFARLTLRDGKPKYLADAPRFIAYIR
                     ATCARYRELTPLLRLVDELEGTQAAMGYAFGRV"
     gene            2021..4474
                     /locus_tag="PK518_05700"
     CDS             2021..4474
                     /locus_tag="PK518_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041836136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HRM48441.1"
                     /translation="MDRSFLLHVPSTRPPMPAALRRPLARLVALMLCGAPLAVLAQVG
                     DGDETPDAPVQPAPALRTSPRLQETLPPQVRQQLPVFVRGDRVTGQPDIRATIEGDAE
                     LRRGDTVIHADHMDYDVPEDRAKASGNVRINRAGNLYDGTLLDLRVDAFNGFFSDARY
                     RFLATQAHGEATRVDFIDHDRSVVHNGTYTTCQRDNEASWQPDWIIRAKTIHLDQAED
                     VGHAEGSVLEFKGVPILPVGSISFPLSDRRKSGLLPPTIGLNSVSGVEYAQPYYWNIA
                     PNRDATITPVLMSRRGVALGSEFRYLEPRYSGELTADIMPRDELRKRDRWAYGVKHRA
                     ALDTPAGDVALGVDVRRVSDDDYWRDFTPRLFSHGSGGAIQSQYVQRLLPGQASLNWA
                     RGEHALSLNTLKWQTLQDVTSPITPPYDRLPQLHWRYQPLQLAGGFDASVEADATVFH
                     ADRRYYAQPNGKRSYAMAQISRPFLAPAGFITPRLQLHATQYQFDDPLANGQTTASRT
                     LPTFSLDSGLVFERDARFFGGDYLQTLEPRAFYTYTPYRDQSLLPVYDTAPNDFNFAS
                     VYTENAFGGNDRIADNNLFTLGVTTRLLNPDSGAEAARFGIAQRLRFSDQRVTMPGAL
                     PVSERVSDLLLGAGINWTPHWGFDSTVQYNPKTRNSQRTTVALRYSPGSFRTLSAAYR
                     MQKVTNLIALPSEQVDVGWQWPLNALGGEGGDKGRAGGRWFSVGRLNYSLQDRKLVDT
                     IVGVEYESCCWIGRVVLERLQSSLISANTRLLFQIEFRGFSRLSLGSNPLASLQRYVP
                     GYQTLGDSTHQPSRFSQYD"
     gene            4527..5906
                     /locus_tag="PK518_05705"
     CDS             4527..5906
                     /locus_tag="PK518_05705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM48442.1"
                     /translation="MTLRVLPLALACLTALTVQAQGLRPSGGGAMRLPSAAGVLPPAG
                     GAASAAVRQADYIVAVVNSEPITNNEVRQRAERVAQQLNGQGAAVPPHEQLAREVLER
                     LILEKIQIQLAKDSGIKVDDFAVDQAEQNVARQNDMSVEEMHRRLAQDGVSPQRFRDD
                     LRNQLLALRVREREVEARVRVSDLEVDQYLQEQKKDAAPGKEELNLGHILIKVAENAS
                     PDEVAQRQARAQQALDKLRSGGDFATLAREYSDAPDGPNGAVLGLRPADRYPELFVNA
                     VRQVAVGDVVGPLRSPAGFHILKVVDKTHGGVPTVAVQNHARHILLRVGAGLSERQAA
                     ERLEELRQRVLRGQADFAALAREYSQDGSAKSGGDLGWAGPGRYVPEFEQALNALRPG
                     EVSQPVVSRFGVHLIELLERREAKLTQREQRDMVRDQVREKKLDEAFTTWIQEARGRA
                     YVEYRDAPQ"
     gene            5903..6673
                     /gene="rsmA"
                     /locus_tag="PK518_05710"
     CDS             5903..6673
                     /gene="rsmA"
                     /locus_tag="PK518_05710"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRM48443.1"
                     /translation="MKHIARKRFGQNFLTDGGIIDAIVRAIDPRPGQPMVEIGPGLMA
                     LTQPLVERLGRLTVIELDRDLAARLRQHGQLDVIESDVLKVDFAQVAQALNAPKIRVV
                     GNLPYNISSPILFHLLEQVHVVQDQHFMLQKEVIDRMVAQPATAAYGRLSVMLQWRYA
                     MEDVLFVPPGSFDPPPRVDSAVVRMLPYDQPAAVDVALLSELVQVAFSQRRKLLRHTL
                     GRWLEARAYAGAFDTQRRAEEVPVADYVALARSVGGRG"
     gene            complement(6917..7342)
                     /locus_tag="PK518_05715"
     CDS             complement(6917..7342)
                     /locus_tag="PK518_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRM48444.1"
                     /translation="METPLRQEQDTLLRGVRPNIVQSIRAFRVHDLQETARVLGQHFL
                     YANLAHAQTKQDVLELIATQFTFPAHFGKNFDALYDCMTDPLHKAGPQPGFVVVLEQI
                     PATVKFDKEAREQLLDIFRDAADYWSDRKIAFRCFYSFL"
     gene            complement(7480..7884)
                     /locus_tag="PK518_05720"
     CDS             complement(7480..7884)
                     /locus_tag="PK518_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HRM48445.1"
                     /translation="MLKAVANRAYKAIVACALGAAVVLAPAGVGARQWQDTPVSSPIA
                     LAELPKEGRATYALIHEGGPFPHDKDGSVFGNRERQLPAKKRGYYREYTVRTPGVAHR
                     GARRIVCGGAKRMPDACYYTSDHYTSFREIRQ"
     gene            complement(8074..10383)
                     /locus_tag="PK518_05725"
     CDS             complement(8074..10383)
                     /locus_tag="PK518_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRM48446.1"
                     /translation="MPNDQTPDTADKRALLRKAALEYHEFPQPGKITVGATKQMINQH
                     DLALAYSPGVAAPCEEIVKDPNAAFKYTARGNLVGVVTNGTAVLGLGDIGPLAGKPVM
                     EGKGVLFKKFSGIDVFDIEINEKDPEKLVEIIASLEPTFGGINLEDIKAPDCFYVERK
                     LRERMKIPVFHDDQHGTAIVVGAAIINGLKVAGKDIKTARLVASGAGAAALACLGLLV
                     KLGLPRENIWVTDIAGVVYEGRAELMDEDKAFFAQKTELRSLSQVIEGADIFLGLSAG
                     GVLKKDMVARMAPRPLIFALANPNPEIAPEDVKAVRDDAIIATGRTDYPNQVNNVLCF
                     PYIFRGALDCGATTITTPMEIAAVHAIAELAQAEQSEVVAKAYAGEALAFGPEYLIPK
                     PFDPRLMMKIAPAVAQAALDSGVALRPIADMDAYRDHLQTFVYASGTTMKPIFTAAKM
                     GTKKRVAYSEGEEERVLRAAQIVVDEHIARPTLIGRPTVIAERIEKFGLRLKEGQDYD
                     VVNVEDDHRYRDFWQTYHRMTERKGVTVPIAKIELRRRLTLIGSMLLHKGEVDGLICG
                     TWGHTAHHLQYIDQVIGRRAGVHTYACMNALLLPNRQLFVVDTHVNYDPTAEQLAEIT
                     IMAAEEILRFGLKPKAALLSHSNFGNSNQPSAVKMRQTLELLRVQAPWLEVDGEMHGD
                     IALDGKARMALMPHSTLAGDANLLVMPNIDAANIAYNLLKTAAGGNIAIGPVLLGAGQ
                     PVHILTPSATVRRIVNMTALTVADANAAR"
     gene            complement(10558..12153)
                     /locus_tag="PK518_05730"
     CDS             complement(10558..12153)
                     /locus_tag="PK518_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRM48447.1"
                     /translation="MTVTRYALAALTWIAVALPAQALDVDRAERLYTRHCAVCHGADR
                     GGYIGPALNRDVTKLTKEQVAGRIVAGKPPTLMPQHPSWRGVLPARDQALLAELITTQ
                     PQRNLSWGMQDIRDSLQVLVAQESTLPAAPTYRITAMNDLMAVMSRSRQGAGKEAKVL
                     FYDGRTHRQVGAVATEFAPHLMDFHPTDPRWAYVRDDAGWVHKVDLYSMRTVRKVRAG
                     LNGISLAVSRDGRYVAAGSYVPHTMVIMDASTLEPLHMIELAGVDPDGRHVSADAGII
                     TATPYANWFVVALEQAGQVWIVDLDQRGMPITTITNVGRHLHDGFLSPDGRHFVVSSY
                     DDNVNTVIDLAQARIVRKIPVGCKPHLGSGALVRSGGRLLGIGTNIGEVDCPSYEVTV
                     FDMESFEVVKRIPVAGPTESPAAHPNAPWIIVDIVGTGPNANKLQFIGKESLEVEHTL
                     EVAGRLGHSHFPEYTARGDFFYVSARYRGDRSQGLPGGQLVIYDAHTLKQVKSIDVDV
                     PAGVFSHVRSRSVTVGLQPPVPH"
     gene            complement(12264..13730)
                     /locus_tag="PK518_05735"
     CDS             complement(12264..13730)
                     /locus_tag="PK518_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRM48448.1"
                     /translation="MKILIARLNHETNTFSPVPTPIAAFSPAYGEEAYHANKGMRTAM
                     AAFIDLAEGLGATLVTPVSAMANPSGPVHAAAYDELTRRIVEAAPGCDAILLDLHGAM
                     VAENSADGEGDLLARVRAAAPGVPLGVALDLHGNITEQMVQNADVMVGFKTYPHIDMY
                     ETGEHAGRLLLQMLRGEASYRVCWHPLPLMSHTLRSTTLEGAMRDAVDAARQLEHGGL
                     PAVTVFAGFSLADIEAPCMSVVATCREEDLDATQAAVDDLAQQIWQRRADYVYRSEAL
                     ADSLRRAANLAQGATRPVLLLDHGDNCMSGGTCDTMDVLQAALAQGLTGIAVGPICDP
                     ETVATLIKAGVGAEVEVALGNKVPLAQLGLHKEPLQLRGRVHTASDGGYTVTGPIYTG
                     QRCHMGRSVCLDIGSAQIVVTERPHEPWDLGVFHCVGVDPTRFRYLLLKSRMYCRPVF
                     VPLSAGLVECDSPGVTTSDYARFQFTHVHRPVYPLDSL"
     gene            complement(13727..14992)
                     /locus_tag="PK518_05740"
     CDS             complement(13727..14992)
                     /locus_tag="PK518_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRM48449.1"
                     /translation="MDRHLPPNAALAALRIDGARLWQSLMDLAQIGATPKGGVCRIAL
                     TDLDRQGRDLFVQWARGAGCSIRVDAIGNIFARRAGQDDGLPPVMTGSHIDTQPTGGK
                     FDGNYGVLAGLEVVRTLNAASVRTRAPIEVAVWTNEEGSRFVPVMMGSGVFAGAFTLE
                     HALAQRDAQGVSVGEALASIGYAGQLGPAPAVGSYFEAHIEQGPVLENHARVIGVVTA
                     ALGQRWYDVTVQGMEAHAGPTPMELRRDALLAASELVLEVNRIAVQRAPHARGTVGTM
                     ELFPNSRNVIPGRASLSVDLRAPDDAQLLDMDAALRAACARIATERSLQITVEQVVYF
                     PPQPFTPQLVQAVRANADDLGYSSMDVVSGAGHDAVYLARVAPAAMIFVPCDDGISHN
                     EIENAAAEHLEAGCNVLLRAMLAAAEVVA"
     gene            complement(15007..15714)
                     /locus_tag="PK518_05745"
     CDS             complement(15007..15714)
                     /locus_tag="PK518_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48450.1"
                     /translation="MKTLLSVESIHAHYGKSHVLQGVSLQVQEGELVTLLGRNGAGKT
                     TTLKTIAGVMPPTQGQVRFAGESTSHLATHQVAQHGICLVPEHRGIFKLLTVEENLLL
                     AQRKKSPWQLADVYRIFPRLQERRTNGGGQLSGGEQQMLAIGRALMNAPRLLMLDEPV
                     EGLAPVIVEEIVAQLKTIKAAGVTILLVEQNLEVCTQLADRHYIIEQGCIVHEAGNAE
                     FLADEAVKDRYLGVGLV"
     gene            complement(15711..16487)
                     /locus_tag="PK518_05750"
     CDS             complement(15711..16487)
                     /locus_tag="PK518_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48451.1"
                     /translation="MSTTAPILLEAQGVAKHYGKFVALGGVDLKVRANTVHSVIGPNG
                     AGKTTLFHMLTGTKAVSGGRILFDGHDVTREPDHRRVRRGMARSFQVTSLFLTLPVRE
                     NLRLAAQGVAPAKALDCWHTPTGPRSCAETVAQVLERVGLTRHAATPAGNLSHGQQRR
                     LEVGMALAAKPKAIFLDEPTSGMGIDDLDDMKRLIQGLKDEHTVVLIEHNMGIVMDIS
                     DTITVMQLGRVLAEGVPAEIRNDERVRTAYLGNMITGGKA"
     gene            complement(16484..17476)
                     /locus_tag="PK518_05755"
     CDS             complement(16484..17476)
                     /locus_tag="PK518_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM48452.1"
                     /translation="MTRHSHLFLALAIVIAMPLFMHSGSLASEVLIYALAAMGCNLLL
                     GYTGLLSFGQGIFFGLGSYTIALLLTRWPLPMPLALLAAVAMGAMGAAVVGWIAIRQR
                     GTYFVMLTLAFAQMFYFLAYSLPGLTGGDNGLLDIPRPSLAVAGQTLWPLVSPWQYYG
                     FVAVLFLLAFWLLRRVCDSMFGRTLLAVRDNEERAAAVGYDLRLLKLQAFVISGAVTG
                     LAGALHAMMTGIAPLSNVEYHTSEMIIVMTVIGGTGNLLASVLGAAFYVLLGDWLSTL
                     WPRWLLLLGVVLMVVSLGLQRGLWGLGESLWALLRRKGPEPATPDAAASNGGQA"
     gene            complement(17482..18342)
                     /locus_tag="PK518_05760"
     CDS             complement(17482..18342)
                     /locus_tag="PK518_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM48453.1"
                     /translation="MSIYLLQTINGIGIGMLYFLLAVGLSIVFGLLRFVNFAHGAFYL
                     IGAYFCYQMTRWGWSFWWTLLLAPLAVGAIGWLTEKLLLRHVYAKPHEFHILVTVGLA
                     LVVQELVILQWGPLGDSVAVPELLQGVVMWGGFVYPKYRLFVIGFTAVLAVLLWWLLE
                     GTRLGSAVRAGSESTEMVSLLGLNVFAIFSLVFALGAATAALAGVLAAPIRGAEPFMG
                     IEALGVAFVIVVVGGLGSFSGALVGGILIGIVQSVMSTLWPEGARLMIYVAMAAVLLL
                     RPHGLLGRKG"
     gene            complement(18425..19630)
                     /locus_tag="PK518_05765"
     CDS             complement(18425..19630)
                     /locus_tag="PK518_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48454.1"
                     /translation="MQRRHLLQLAALGALPSSLSLASTAWAQAKDAIQFGCPVPMSGP
                     FAANGKFADLGMKLAIEQYGKALGRPLAYTLLDTEGKPATAVRKVQESAQQQGARFFA
                     GGILSSEALAMGKEAEKAGGVFITTAGADEITGKDCNSATFRWSVPTFGAIEQTVRPL
                     VEAMPKAKRWYTITPQYVFGDGLLSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNA
                     VAAKPDVLLLLNFGAQSSDTLRQAISFGMHKNMTILIAWASGLEQFESLGADLCDGVY
                     FGAQYWHTADAPQNLDLVKRCQDKFKANPNYSLAGSYICTKLLIDAIVKAGSVEQKAV
                     ITALEGMKYQGLTGEEEVRKADHQVLKNYYLLKGKAKAKMKNKDDYVDVISSGKSFLP
                     VDQTGCKLA"
     gene            19793..20605
                     /locus_tag="PK518_05770"
     CDS             19793..20605
                     /locus_tag="PK518_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRM48455.1"
                     /translation="MTKPKTSSLAIKQLKRSDLVAQEVKRLITEKNLSPGDRLPREIE
                     LQEQFQVSKGTIREALKSLEVQGLITISTGPTGGGTIAEVPLDRTLQFMQNYLFFQEV
                     TIDDIYTVRQMLEPELAAGAVPHLTEADFEALEHSISCCDPTQSHEDLLTQRREDVNF
                     HDILAAANPNPFLRFSCELINEMIRQLIVFGNRTPQTEHRRFGEANAQFHRDIVQAAR
                     ARDAERVRVLMHQHMQDAASSVKRMKGRIQGRLILDADTLRRPRPVAGRKRA"
BASE COUNT         3464 a         6882 c         6908 g         3440 t
ORIGIN      
        1 cgcagcgggc ggctgccgtc ccgcagcgac agcagcaggc gcgtgccctc agattggccg
       61 ccgcgcagcc agtcggccag cgccagcggc gcatgcacgg ggggcacgcg gttgcgcccg
      121 cattgctcgc aggcggccac cgctatcgcc tgccagtgag cctggcgctt ggcggcgcgc
      181 tcgccggcca gcttgactac gctgcgtgcc gtggtcacgg gctggatgct ggcggcgccg
      241 agttcggtgg ccttttccac cagccagtcc atgcgctcgt tggccggcat gccgaccacc
      301 agatgcacgg cgcgcgcggc ctcgcgctcg acggcggtgt gcgcgccaat ctctacatcg
      361 acatcgctgc ggcccatgcg cgtgacgctg gccgtccact cgccgccctc accgttgaac
      421 agcgtgatga cgccgcccgg ctgcaggcgc agcacctgca catggcgcgc ggcctggggc
      481 ggcagcgtga cttgcgcgcc ggcggccagg ggcagggggc agtaaaaacg tggcattcag
      541 tttaaaaata gtagctgtta gcgcttggta gataagtgtt gcaggctgtt ttgatgatga
      601 aaaccatggc cgctgcgttg gcgcctgttt ctccctctcc cgcgtgcggg agagggcggg
      661 ggtgagggtg taaaaacggg gcgctgcgcc gcatcacccc ctcaccccaa ccctctcccc
      721 caagggggcg agggagctaa aacctgcgct aaatatgcgt tacgcgctat caaaaagatg
      781 tgtgcataca caaacccgcg aggggggaag aaaaccaatg caccgcgccg ttgttaaacc
      841 cggccgaagg cgtagcccat ggccgcctgc gtgccctcca gttcatcgac caggcgcagc
      901 agcggcgtca gctcgcggta gcgtgcgcag gtggcgcgga tgtaggcgat gaagcgcggc
      961 gcgtccgcca ggtatttggg cttgccgtcg cgcagcgtca ggcgggcgaa gatgccggcc
     1021 accttcaggt ggcgctgcag cgccatgaag tccacggcgc ggtagaactc gccgaagtcc
     1081 gcaccccagc cgctggcgct tgcggcgccg atgaggccgg ccttgcgggc cttttcccag
     1141 taacgcacgg taatgtcgat gacgaaatcc tcttcccagc tgatgaaggc gtcgcgcatc
     1201 aggctggcga tgtcgtaggt cacggggcca aagacggcgt cctggaagtc gagcacgctc
     1261 aagcgtccat cggcgcgcac catcaggttg cgcatcatga agtcgcggtg cacatagacg
     1321 ctgggcgcgg ccaggttctg cgccactatt gtttcgaagg ccttggccag cgtggcctgc
     1381 tgggcgtcgg tgagcgttac ctggcgatgg cgcgcgatgt accagtcggg gaacagctgc
     1441 agctcgcggc gcaacagcgc gtcgtcgtag gccggcagca cgccggggcg gctggccttt
     1501 tgccagtcaa tcagcagatc cacggcctgc aggtaccagg cgtgggcggc ggcgggttgg
     1561 gcctcgtcca accgctctat ggcggtctgc acgcctaagt cggacagcag catgaagcca
     1621 tgcgtttgat cccaggcgtg gatctgcggc accggcaggc cggcggactg catcaggccc
     1681 tgcacatgca cgaaggggcg gcagtcctcc ttgtcgggcg gggcgtccat gacgatgcga
     1741 ctgctgccgt cctgcgcgtc caggcgcaga taacggcgaa agctggcgtc ggcggatgcg
     1801 gggcgcaggc tggcgggcag caggttctgc gcggcggcta tcggggccag ccaggccagg
     1861 aaggcggatt ggcgcgcggg gtcgttccat tgcacggcgc tggcgccgct ggggaggggg
     1921 gggaggggat ggctcatgga taatcggatt ttacaaaccc ggtcttccct ggccgggcgg
     1981 cacatcgccc ggcgtgccat gtgccagcct cttgcaatcc atggatcgtt ccttcctgct
     2041 ccacgtcccg tccacccgcc cgcccatgcc ggccgcgttg cgccgtccgc tagcgcggct
     2101 ggtggccttg atgctgtgcg gcgcgccgct ggccgtgctg gcccaggtgg gcgacggcga
     2161 tgagacgccc gacgcgcctg tccaaccggc ccccgcgctg cgcaccagtc cgcggctgca
     2221 ggaaaccctg ccgccccagg tgcgccagca gctgccggtg ttcgtgcgtg gcgaccgcgt
     2281 cacgggccag cccgacatcc gcgccaccat cgagggcgat gccgagctgc gccgcggcga
     2341 cacggtgatc cacgccgacc atatggacta cgacgtgccc gaggaccgtg ccaaggccag
     2401 cggcaacgtg cgcatcaacc gcgccggcaa cctgtacgac ggcacgctgc tggatctgcg
     2461 cgtggacgcc ttcaacggct ttttcagcga tgcgcgctac cgctttctgg ccacccaggc
     2521 ccatggcgag gccacgcgtg tggacttcat agaccatgac cgttcggtgg tgcacaacgg
     2581 cacctacacc acctgccagc gtgacaacga ggccagttgg cagcccgact ggatcatccg
     2641 cgccaagacc atccacctcg accaggccga ggacgtgggc catgccgagg gcagcgtgct
     2701 ggagttcaag ggcgtgccca tcctgccggt gggcagcatc agctttccgc tgtcggacag
     2761 gcgcaagtcc ggcctgctgc cgccgaccat aggcctgaac agcgtcagcg gcgtcgaata
     2821 tgcccagccg tactactgga acatcgcccc gaaccgcgac gccaccatca cccccgtgct
     2881 gatgagccgg cgcggcgtgg ccctgggcag cgagttccgc tacctggagc cgcgctacag
     2941 cggcgagctg accgccgaca tcatgcccag ggacgagctg cgcaagcgcg accgctgggc
     3001 ctatggcgtc aagcaccgcg cggcgctgga cacccccgcg ggcgacgtgg ccctgggcgt
     3061 ggatgtgcgc cgcgtgagcg acgacgacta ctggcgcgac ttcacgccgc gcctgttcag
     3121 ccatggcagc ggcggcgcca tccagtccca gtatgtgcag cgcctgctgc cgggccaggc
     3181 gtcgctgaac tgggcgcgtg gcgagcatgc gctgagcctg aacacgctca agtggcagac
     3241 cctgcaggac gtgacctcgc ccatcacccc gccctatgac cgcctgccgc aactgcattg
     3301 gcgctaccag ccgctgcagc tggccggcgg ctttgacgcc agcgtggagg ccgatgccac
     3361 cgtcttccat gccgaccgca ggtattacgc ccagcccaac ggcaagcgca gctatgccat
     3421 ggcgcagatc agccggccct ttctggcgcc ggccggcttc atcacgccgc gcctgcagct
     3481 gcatgccacg cagtaccagt tcgacgatcc gctggcaaac ggccagacca ccgccagccg
     3541 caccctgccc accttcagcc tggacagcgg cctggtgttc gagcgcgatg cgcgcttctt
     3601 tggcggcgac tacctgcaga cgctagagcc gcgcgccttc tacacctaca cgccctaccg
     3661 tgaccagagc ctgctgccgg tgtatgacac ggcgcccaac gacttcaact tcgcctcggt
     3721 ctataccgag aacgcctttg gcggcaacga ccgcatcgcc gacaacaacc tgttcacgct
     3781 gggcgtgacc acgcgcctgc tgaaccccga cagcggcgcc gaggcggcgc gctttggcat
     3841 cgcccagcgc ctgcgctttt ccgaccagcg cgtcaccatg cccggcgcgc tgccagtcag
     3901 cgagcgcgtg tccgacttgc tgctgggcgc gggcatcaac tggacgccgc actggggttt
     3961 tgactccacc gtgcaataca accccaagac gcgcaactcg cagcgcacca cggtggcgct
     4021 gcgctactcg ccgggcagct ttcgcaccct gagcgcggcc taccgcatgc agaaggtgac
     4081 caacctgatc gccctgccca gcgagcaggt cgatgtgggc tggcaatggc ccctcaacgc
     4141 ccttggcggc gagggcggcg acaagggtcg cgccggcggg cgctggttca gcgtgggccg
     4201 gctcaactac agcctgcagg atcgcaagct ggtggacacc atcgtcggcg tggaatacga
     4261 gagctgctgc tggataggcc gcgtggtgct tgagcgcctg caaagcagcc tgatcagcgc
     4321 caacacccgg ctgctgttcc agatcgagtt ccggggcttt tcgcgcctct cgctgggctc
     4381 caaccccctg gcctccctgc agcgctatgt gccgggctac cagaccctgg gcgacagcac
     4441 gcaccagccc agccgcttca gccagtacga ttaaccgcac gcgcgctcag cgcggatcgc
     4501 cttgcaccat gaatttctct cacgccatga cactccgcgt tctgcccctg gcactggcct
     4561 gcctgacggc cctgacggtt caggcccagg ggctcaggcc ttcgggcggt ggggccatgc
     4621 gcctgccgtc agcggccggc gtgctgcctc ccgcgggcgg cgccgccagt gcggcggtgc
     4681 gccaggccga ctacatcgtc gccgtggtca actccgagcc catcaccaac aacgaggtgc
     4741 gccagcgtgc cgagcgcgtg gcgcagcagc tgaacgggca gggcgcggcc gtgccgcctc
     4801 acgagcagct ggcccgggag gtgctggagc gtctgatcct ggaaaaaatc cagatccagc
     4861 tggccaagga cagcggcatc aaggtcgatg actttgccgt ggaccaggcc gagcagaacg
     4921 tggcgcggca aaacgacatg agcgtcgagg aaatgcaccg ccgcctggcc caggacggcg
     4981 tgagtccgca gcgctttcgc gacgacctgc gcaaccagct gctggccctg cgcgtgcgcg
     5041 aacgcgaggt ggaggcgcgc gtgcgcgtga gcgacctgga ggtcgatcaa tacctgcagg
     5101 agcagaaaaa ggacgcggcg ccgggcaagg aagagctgaa cctgggccat atcctcatca
     5161 aggtggccga gaacgccagc cccgacgagg tcgcccagcg ccaggcgcgc gcccagcagg
     5221 cgctggacaa gctgcgctcc ggcggcgact ttgccacgct ggcgcgtgaa tattccgacg
     5281 ccccggacgg gccgaacggt gccgtgctgg gcctgcgccc ggccgatcgc tatcccgagc
     5341 tgttcgtcaa cgccgtgcgg caggtggcgg tgggtgatgt ggtcgggccg ctgcgctcgc
     5401 ccgcgggctt ccacatcctg aaagtcgtgg acaagaccca tggcggcgtg cccacggtgg
     5461 ccgtgcagaa ccatgcgcgc cacatcctgc tgcgcgtggg cgcgggcctg tccgagcgcc
     5521 aggcggccga acgcctggaa gaactgcgcc agcgcgtgct gcgtggccag gccgactttg
     5581 ccgcgctggc gcgcgagtac tcgcaggacg gcagcgccaa gagcggtggc gacctgggct
     5641 gggccgggcc gggccgctat gtgccggagt tcgagcaggc gctcaatgcc ctgcgccccg
     5701 gcgaggtgag ccagcccgtg gtgtcgcgct ttggcgtgca cctgatcgag ctactggagc
     5761 gccgcgaggc caagctcaca cagcgcgagc agcgcgacat ggtgcgtgac caggtgcgcg
     5821 agaagaagct cgacgaggcc ttcaccacct ggatccagga ggcgcgcggc cgcgcctacg
     5881 tcgaataccg cgacgcaccg caatgaagca cattgcccgc aagcgcttcg gccaaaactt
     5941 tctgaccgac ggcggcatca tcgatgccat cgtgcgcgcc atcgacccgc gtccgggcca
     6001 gcccatggtc gagatcggcc cgggtctgat ggcgctgacc cagccgctgg tcgagcgcct
     6061 gggccggctc acggtgatcg agctggaccg cgacctggcc gcgcgcctgc gccagcacgg
     6121 ccagctcgat gtcatcgaat ctgatgtgct aaaagtggat tttgcgcagg tggcgcaagc
     6181 gctgaatgct cctaaaataa gagtagtcgg caacctgccc tataacatct ccagccccat
     6241 cctgtttcac ctgctggaac aggtgcacgt ggttcaggat cagcacttca tgctgcagaa
     6301 agaggtcata gaccgcatgg tggcccagcc ggccacggcc gcctatggcc ggctgtcggt
     6361 gatgctgcag tggcgctacg ccatggaaga tgtgttattc gtgccgccgg gcagctttga
     6421 cccgcccccg cgcgtggaca gcgccgtggt gcgcatgctg ccctacgacc agcccgcggc
     6481 ggtggacgtg gcgttgctgt ccgagctggt gcaggtggcc ttcagccagc ggcgcaagct
     6541 gctgcgccat accctcgggc gctggctgga ggcgcgcgcc tacgccggag ccttcgatac
     6601 ccagcgccgc gccgaggagg tgccggtggc ggattacgtg gcgctggcgc gcagcgtggg
     6661 gggaagaggt tgagcgttcg gcgttgagcg ttcggcggct gttcgcgctc aacgccccag
     6721 ccagactatg ccgctgccag ccagtatcct acgttgaacg ggctgctcat gcgcagggcc
     6781 agcggggaca catccaccag cttgtcggtg ggcatcttct cgcccgtggg ggtgatttca
     6841 atctgcgtgg cctgggaggc agcgccttgg cgcggttcct cagcccgttc cgcctggccg
     6901 gcggttgcag aacgggctac agaaaagaat agaagcagcg gaaggcgatc ttgcggtcgc
     6961 tccagtagtc ggcggcatcg cggaagatgt ccagcagttg ctcacgcgct tccttgtcaa
     7021 acttcaccgt ggccgggatc tgctcgagca ccacgacaaa gcctggctgc gggccggcct
     7081 tgtgcagcgg gtcggtcatg cagtcgtaca gcgcgtcgaa gttcttgccg aaatgggccg
     7141 ggaaggtgaa ctgcgtggcg atcagctcca gcacatcctg cttggtctgg gcatgggcca
     7201 ggttggcata caggaaatgc tggcccagca cgcgcgccgt ctcctgcagg tcgtgtacgc
     7261 gaaaggcgcg tatcgactgc acgatgttcg ggcgcacgcc gcgcaacagc gtgtcctgct
     7321 cttgacgaag tggcgtttcc atctccgttg ctctttctct tagagattca cgaattacag
     7381 tgtccgcaag cgccagcgag ctgtcctgcg aatgaaaagg gaaatggtgc tccacatcgg
     7441 atcgcaccat gaaatctgcg cttgaagaca tcatcgggtt cattgcctga tttctcgaaa
     7501 gctcgtgtag tggtcgctgg tgtaatagca ggcatcgggc atgcgtttgg cgccgccgca
     7561 gacgatgcgc ctggcgcccc ggtgtgccac ccctggtgtg cgcaccgtgt actcccggta
     7621 gtagccgcgc ttcttggcgg gcagctgccg ctcgcgattg ccgaacaccg aaccatcctt
     7681 gtcgtgcgga aacggcccgc cctcgtggat cagcgcatag gtggcacgcc cctccttggg
     7741 cagctcggcc agcgcgatgg gggaggagac gggggtgtcc tgccattgcc gcgcgcccac
     7801 gccagccggt gccagcacca ccgcagcacc cagagcgcac gccacaattg ccttgtacgc
     7861 ccggttggcg acagccttca acatccacta cacctttcag aaattgcggg ttaacccgaa
     7921 atttcaacgg gttagtgtga caaaaacgcc accaaaaggc aagcgcctgc ccaaaaaatg
     7981 ggcaggcgca aaaggttaat tatcctgaat cccgcagcag gcgcgctgcg catggccctg
     8041 acgggggcag ggcgcaggat tcgggagcgc ggcttaccgc gccgcgttgg cgtcggccac
     8101 cgtcagggcc gtcatgttga cgatgcggcg cacggtagcg ctgggggtga ggatatgcac
     8161 gggctggccc gcgcccagca gcaccgggcc gatggcgatg ttgccgccgg cggcggtctt
     8221 gagcaggttg taggcaatat tcgccgcatc gatgttgggc atcaccaaca gattggcgtc
     8281 gcccgccagc gtgctgtgcg gcatcagggc catgcgcgcc ttgccgtcca gggcgatgtc
     8341 gccatgcatt tcgccgtcca cctccagcca gggggcctgc acgcgcagca gctccagcgt
     8401 ctggcgcatc tttacggcgc tgggctggtt gctgttgcca aagttggagt gcgacagcag
     8461 cgccgccttg ggcttgaggc caaagcgcaa tatctcctcg gccgccatga tcgtgatctc
     8521 ggccagctgc tcggccgtgg ggtcgtagtt cacatgggtg tccaccacga acagctggcg
     8581 attgggcagc agcagggcgt tcatgcaggc ataggtgtgc acgccggcgc gccggccaat
     8641 cacctggtcg atgtactgca ggtggtgtgc ggtgtggccc caggtgccgc agatcaggcc
     8701 gtccacctcg cccttgtgca gcagcatgga gccgatcagc gtcaggcgcc ggcgcagctc
     8761 gatcttggcg atcggcaccg tgacgccctt gcgctcggtc atgcggtggt aggtctgcca
     8821 gaagtcgcgg tagcggtggt cgtcctccac gttcaccacg tcgtagtcct ggccctcctt
     8881 caggcgcagg ccgaactttt cgatgcgctc ggcaatcacc gtcgggcggc cgatcagcgt
     8941 gggccgggcg atatgttcgt ccaccacgat ctgtgccgcg cgcagcacgc gctcttcctc
     9001 gccctcggag taggcaacgc gcttcttcgt ccccatcttg gccgccgtga agatgggctt
     9061 catggtcgtg ccagaggcgt agacgaaggt ctgcaggtgg tcgcggtagg catccatgtc
     9121 cgcgatcggg cgcagcgcca cgccggaatc caacgcggcc tgggccacgg ccggcgcgat
     9181 cttcatcatc aggcgcgggt cgaacggctt ggggatcagg tactcggggc cgaaggccag
     9241 cgcctcgccc gcataggcct tggccaccac ctcgctctgc tcggcctggg ccagctcggc
     9301 gatggcgtgc accgccgcga tctccatcgg cgtggtgatg gtggtggcgc cgcaatcgag
     9361 cgcgccgcgg aagatgtagg ggaagcacag gacgttgttg acctggttcg ggtagtcggt
     9421 gcggccggtg gcgatgatgg catcgtcacg cacggccttc acgtcctcgg gcgcgatctc
     9481 gggattcggg ttggccagcg cgaagatcaa tgggcgcggc gccatcctgg ccaccatgtc
     9541 cttcttcagc acgccgccgg cggacaggcc caggaagatg tcggcgccct caatcacctg
     9601 ggacaggctg cgcagctcgg tcttttgcgc gaagaaggcc ttgtcctcgt ccatgagctc
     9661 ggcccggccc tcgtagacca cgccggcgat gtcggtgacc cagatgttct cgcgcggcag
     9721 gcccagcttg accagcaggc ccaggcaggc cagcgccgcg gcgcccgcac ccgaggccac
     9781 gagcctggct gtcttgatgt ccttgcccgc caccttcagg ccgttgatga tggcagcgcc
     9841 gacgacgatg gccgtgccgt gctgatcgtc gtgaaagacc gggatcttca tgcgctcgcg
     9901 cagcttgcgc tcgacgtaga agcagtccgg cgccttgatg tcttccaggt tgatgccgcc
     9961 gaaggtgggc tccaggctgg cgatgatctc gaccagcttc tcggggtctt tctcgttgat
    10021 ctcgatgtcg aacacgtcga tgcccgagaa cttcttgaaa aggacgccct tgccctccat
    10081 caccggcttg ccggccagcg gcccgatgtc gcccaggccg agcaccgccg tgccgttggt
    10141 caccacgcca accaggttgc cgcgcgcggt gtacttgaag gcggcgttcg ggtccttgac
    10201 gatctcctcg cagggcgcgg ccacgccggg cgaataggcc agcgccaggt catgctggtt
    10261 gatcatctgc ttggtggcgc caacggtgat cttgccgggc tgggggaact cgtggtattc
    10321 gagcgcggcc ttgcgcagca gggcgcgctt gtctgcggtg tcaggggtct ggtcgttggg
    10381 cattgttgtt tctccggcgc aagccattcc aaatccgaac cgccgggtgg aatgtcagcg
    10441 gcgcattgag cggcgattgt aggactgccc tcttgatgca cccatgcgcg caaggcgctt
    10501 gcagggcgaa acagttttgc cgaggcagcg gtgaatgggt cagggcgtga gtgagtgtca
    10561 gtgcggtaca ggcggttgca gccctacggt caccgagcgc gaacgaacat gcgagaacac
    10621 gcccgcagga acgtccacgt caatggactt gacctgtttg agcgtgtgcg cgtcgtaaat
    10681 caccagctgt ccacctggca ggccctggct gcgatctccg cgatagcgcg cgctgacgta
    10741 gaaaaaatcg ccgcgcgcgg tgtactctgg aaaatgcgaa tgaccaagcc ggccggccac
    10801 ctccagcgtg tgctccacct cgagcgactc cttgccgatg aattgcagct tgttggcatt
    10861 cgggccggtg ccgacgatgt ccacgatgat ccagggcgca ttcggatgtg cggccggcga
    10921 ctcggtgggc cctgccaccg ggatgcgctt gaccacttcg aagctttcca tgtcgaacac
    10981 ggtcacctcg taggacgggc agtccacctc gccgatgttg gtacctatgc ccagcaggcg
    11041 cccgcccgag cgcaccaggg ctccagagcc caggtgcggc ttgcagccca ccgggatctt
    11101 gcgcacgatg cgcgcctggg ccaggtcgat cacggtgttg acgttgtcgt cgtacgagga
    11161 gaccacgaaa tgccggccat cgggcgagag aaaaccgtcg tgcagatggc gcccgacgtt
    11221 ggtgatggtg gtgatgggca tgccgcgctg atccagatcc acgatccaca cctgccccgc
    11281 ctgctccagc gccacgacga accagttggc gtaaggcgtg gcggtgatga tgccggcatc
    11341 agccgacaca tgcctgccgt ccgggtcgac gcccgcaagc tcgatcatgt gcagcggctc
    11401 cagcgtgctc gcgtccatga tcaccatggt gtgcgggacg taggagcccg ccgccacata
    11461 gcgcccgtca cgcgaaaccg ccagtgaaat cccgttcaac cccgcacgca ccttgcgcac
    11521 ggtgcgcatg gagtacagat ccaccttgtg cacccagccg gcatcgtcgc gcacataggc
    11581 ccagcgcggg tcggtgggat ggaaatccat caggtgtggc gcaaattccg tcgccaccgc
    11641 acccacctgg cggtgcgtgc gcccgtcgta gaacagcacc ttggcttcct tgcccgctcc
    11701 ctggcggctg cgcgacatga cggccatcag gtcgttcatg gccgtgatgc gataggtggg
    11761 cgcagccggc agggtggact cctgcgctac cagcacttgc agagagtcgc gaatgtcctg
    11821 catgccccag gacaggttgc gttgcggctg ggtggtgatg agctcggcca gcagggcctg
    11881 atccctggcg ggcaagacgc cgcgccatga gggatgctgc ggcatcagtg tcggtggctt
    11941 gcccgccaca atgcggccgg ccacctgctc cttcgtgagt ttggtgacgt cccggttcag
    12001 cgccggtcct atgtagccac cacgatcagc accgtggcat accgcgcaat gccgggtgta
    12061 caggcgctcg gcacgatcaa cgtccagagc ctgtgcgggc agggcgaccg caatccatgt
    12121 gagtgcagcg agggcgtagc gcgtcactgt cattgcagac ctcccggtgt ccacgacggc
    12181 cgaaccatag ccactgccag catgctgtca agcgatgaca ttcgccttga tgtggcgcaa
    12241 cccagtccag atgcgcagct tgtttagagc gagtcgagcg gatagacggg acgatgcaca
    12301 tgcgtgaact ggaagcgcgc atagtccgag gtggtgacgc ctgggctgtc gcattccacc
    12361 aggccggcgg acagcggcac gaacaccggc cggcagtaca tgcgcgactt gagcagcagg
    12421 tagcgaaagc gcgtgggatc cacgcccacg caatggaata cgcccagatc ccagggctcg
    12481 tgcggccgct cggtgaccac gatctgtgcg ctgcctatgt ccaggcatac cgagcggccc
    12541 atgtggcagc gttggccggt gtagatcggg ccggttacgg tgtagccgcc atcgctggcc
    12601 gtatgcaccc ggccgcgcag ttgcagcggc tctttgtgca ggcccagttg agccaacggc
    12661 accttgttgc ccagggcgac ctccacctcg gcgcccaccc cggccttgat cagagtggcc
    12721 acggtctcgg ggtcgcagat ggggcccacg gcgatgcccg tgaggccctg tgccagggcc
    12781 gcctgcagca cgtccatggt gtcgcaggtg ccgccggaca tgcagttgtc gccatggtcc
    12841 agcagcagca cggggcgcgt ggcaccctgg gccaggtttg cggcgcggcg cagcgaatcg
    12901 gccagcgctt cgctgcggta gacatagtcg gcgcggcgct gccagatctg ttgtgccagg
    12961 tcgtccacgg cagcctgggt tgcgtccaga tcctcctcgc ggcaggtggc gaccacgctc
    13021 atgcagggcg cctctatgtc ggccagagag aagccggcga acacggtcac cgccggcagg
    13081 ccgccgtgtt ccagctggcg tgcggcatcg acggcatcgc gcatggcgcc ctccagcgtg
    13141 gtgctgcgca gcgtgtggct catcagcggc agcgggtgcc agcacacgcg gtaactggcc
    13201 tcgccgcgca gcatctgcag cagcagacgg ccggcgtgct cgccggtttc gtacatgtcg
    13261 atatgcgggt aggtcttgaa gcccaccatc acatcggcgt tctgcaccat ctgctcggtg
    13321 atgttgccgt gcagatccag tgccacgccc aggggcacgc cgggcgctgc ggcgcgcacg
    13381 cgcgccagca ggtcgccttc gccgtcggcg ctgttctcag ccaccatggc gccgtgcaga
    13441 tccagcagga tggcgtcgca gcccggggcg gcttccacga tgcggcgcgt cagctcatcg
    13501 taggccgcgg cgtgcaccgg gccgctgggg ttggccatgg ccgacaccgg cgtgaccagc
    13561 gtcgcgccca ggccctcggc caggtcgatg aaggccgcca tggcggtgcg catgcccttg
    13621 ttggcgtggt aagcctcttc accataggcg ggagaaaacg ccgcgatggg cgtgggcacg
    13681 ggcgaaaagg tgttggtctc gtggttcagc cgcgctatca ggatcttcat gccacgacct
    13741 ccgccgccgc cagcatggcg cgcagcagca cgttgcagcc ggcctccagg tgctcggctg
    13801 cagcgttctc gatctcgttg tggctgatgc cgtcgtcgca gggcacgaag atcatggccg
    13861 cgggcgccac gcgcgccagg tacacggcgt cgtggccggc gccgctgacc acgtccatgc
    13921 tgctatagcc caggtcgtcg gcgttggcgc gcacggcctg caccagctgc ggcgtgaacg
    13981 gctggggcgg gaagtagacc acttgctcca ccgtgatctg caggctgcgt tccgtggcga
    14041 tgcgcgcgca ggcggcgcgc agtgcggcat ccatatccag cagctgtgca tcgtcgggcg
    14101 cgcgcaaatc cacgctcagg ctggcacgac ccggaatcac gttgcgtgaa ttcgggaaca
    14161 gctccatcgt gcccacggtg ccgcgcgcat gcggggcacg ctgcactgct atgcggttca
    14221 cctccagcac cagttcgctg gcggccagca gcgcgtcgcg gcgcagctcc atgggcgtgg
    14281 ggccggcgtg ggcctccatg ccctggacgg tgacgtcgta ccagcgctgg cccagcgcgg
    14341 cggtgaccac gccgatcacg cgcgcgtggt tctccagcac cgggccctgc tcgatatgag
    14401 cctcgaaata cgaacccacg gcaggtgccg ggccaagctg gcccgcatag cctatggatg
    14461 ccagcgcctc gcccacgctc acgccctgcg cgtcgcgctg cgccagcgca tgctccagcg
    14521 tgaaggcgcc ggcaaacacg cccgagccca tcatcaccgg cacgaagcgc gagccttcct
    14581 cgttggtcca caccgcgacc tcgatgggcg cacgcgtgcg cacgctggcg gcgttgagcg
    14641 tgcgcacgac ttcgaggccg gccagcacgc cgtagttgcc gtcgaacttg ccgcccgtgg
    14701 gctgggtgtc gatgtggctg ccggtcatca cgggtggcag gccgtcgtcc tggcccgcgc
    14761 ggcgcgcgaa gatgttgccg atcgcgtcca cgcggatgct gcagccggcg ccccgcgccc
    14821 attgcacgaa caggtcgcgg ccttggcgat ccaggtcggt gagggcgatg cggcacacgc
    14881 cccccttggg tgtggcgccg atctgcgcca ggtccatcag cgactgccac agccgcgcgc
    14941 cgtctatgcg cagagcggcc agggccgcat tggggggaag atgcctgtcc atggaaacta
    15001 ctcctttcac accaggccta cgcccaggta gcgatccttg acggcctcgt cggccagaaa
    15061 ttcggcgttg ccggcctcgt gcacgatgca gccctgttcg atgatgtagt ggcgatcggc
    15121 cagctgcgtg cagacctcca ggttctgctc caccagcaaa atggtcacgc ccgcagcctt
    15181 gatggtcttg agctgggcca cgatctcctc gacgatcacc ggggccagac cttccacggg
    15241 ctcgtccagc atcagcaggc gcggcgcgtt catcagcgcg cggccaatgg ccagcatctg
    15301 ctgctcgccg cccgagagct ggccgccgcc gttcgtgcgg cgctcctgca ggcgcgggaa
    15361 gatgcggtac acgtcggcca gctgccaggg cgacttcttg cgctgcgcca gcagcaggtt
    15421 ttcctccacc gtgagcagct tgaagatgcc gcggtgctcg ggtaccaggc agatgccgtg
    15481 ctgtgccacc tggtgcgtgg ccaggtggct tgtggattcg ccggcaaagc gcacctggcc
    15541 ctgcgtgggc ggcatcacgc cggcaatggt tttgagcgtg gtggtcttgc cggcgccgtt
    15601 tcggcccaac agggtgacga gctcgccctc ctgcacctgc agcgacacgc cctgcagcac
    15661 atggctcttg ccgtaatggg cgtgtatgga ctccaccgac agcaatgtct tcatgccttg
    15721 cctccggtga tcatattgcc caggtaggcc gtgcgcacgc gctcgtcgtt gcggatctcg
    15781 gcgggcacgc cctcggccag cacgcggccc agctgcatga cggtgatggt gtcggagatg
    15841 tccatgacga tgcccatgtt gtgctctatg agcaccacgg tgtgctcgtc tttcaggccc
    15901 tgtatcagcc gcttcatgtc gtcgagatcg tcaatgccca tgccggacgt gggctcgtcc
    15961 aggaagatgg ccttcggttt cgcggccagg gccatgccca cttccagccg ccgctgctgg
    16021 ccgtgcgaga ggttgccggc cggcgtggcg gcgtggcgcg tgaggcccac gcgctccagc
    16081 acctgggcca cggtctcggc gcaggagcgc ggccccgtgg gcgtgtgcca gcaatccagc
    16141 gccttggccg gtgccacgcc ctgggccgcc aggcgcaggt tttcgcgcac cggcagggtc
    16201 aggaacaggc tggtgacctg gaaggagcgc gccatgccgc ggcgcacgcg ccggtggtcg
    16261 ggctcgcgcg tcacgtcatg gccgtcgaac aggatgcgac cgccgctcac ggccttggtg
    16321 ccggtgagca tgtggaacag cgtggtcttg cccgcgccgt tggggccgat caccgaatgc
    16381 acggtgttgg cgcgcacctt caggtccacg ccgcccaggg cgacgaactt gccgtaatgc
    16441 ttggccacgc cctgggcttc gagcaggatg ggtgcggtgg tgctcatgcc tggcctccgt
    16501 tcgatgcggc ggcgtcgggt gtggcgggct ccggcccctt gcggcgcagc agcgcccata
    16561 ggctctcgcc caggccccac aggccgcgct gcaggcccag gctcacaacc atcagcacca
    16621 cgcctagcag cagcagccag cgcggccaca gcgtggacag ccagtcgccc agcagaacgt
    16681 agaaggccgc gcccagcacc gaggccaaca gattgccggt gccgccgatg acggtcatca
    16741 cgatgatcat ctcgctggtg tggtactcca cgttggacag cggcgcgata ccggtcatca
    16801 tggcgtgcag ggcgccggcc aggccggtga cggcgcccga gatcacgaag gcctgcagct
    16861 tcagcaggcg caggtcgtaa cccacggcgg cggcacgctc ctcgttgtcg cgcacggcca
    16921 gcagcgtgcg gccgaacatc gaatcgcaga cgcggcgcag cagccagaac gccagcagga
    16981 acagcacggc cacgaagccg tagtactgcc agggcgagac cagcggccac agcgtctggc
    17041 cggcgacggc gagcgacggg cgcggtatgt cgagcagacc gttgtccccg cccgtcaggc
    17101 cgggcagcga ataggccagg aaatagaaca tctgcgcgaa tgccagcgtc agcatgacga
    17161 agtaggtgcc gcgctggcga atcgcgatcc agcccaccac ggccgcgccc atggccccca
    17221 tggccacggc cgccaacagc gccagcggca tgggcagcgg ccagcgcgtg agcaacaagg
    17281 cgatggtgta gctgcccagg ccgaagaaga tgccctggcc gaacgacagc aggcccgtgt
    17341 agcccagcag caggttgcag cccatggcgg cgagggcgta gatcagcacc tcgctggcca
    17401 gcgaacccga gtgcatgaac agcggcatgg cgatgacgat ggccagtgcg aggaaaaggt
    17461 gggaatgcct ggtcatgggg atcatccttt gcggcccagc aggccatggg ggcgcagcag
    17521 cagcacggcg gccatggcca cgtaaatcat caggcgtgcg ccttcgggcc acagcgtgct
    17581 catcacgctt tgcacgatgc cgatgagaat gccgcccacc agcgccccgc tgaagctgcc
    17641 cagcccgccc accaccacga tgacgaaggc cacgccgagc gcctctatgc ccatgaaggg
    17701 ctcggccccg cgtatcggcg cggcgagcac gccggccagc gccgccgtgg ctgcaccgag
    17761 cgcgaacacc aggctgaaga tggcgaacac gttcaggccc agcagcgaca ccatctcggt
    17821 ggattcgctg ccggcgcgca ccgcgctgcc caggcgcgtg ccctccagca gccaccacag
    17881 cagcacggcc agaacggccg tgaagccgat cacgaacagc cggtacttgg ggtagacaaa
    17941 gccgccccac atgaccacgc cctgcagcag ctccggcacg gccacgctgt cgcccagcgg
    18001 cccccactgc aggatcacga gctcctgcac caccagggcc aggcccacgg tgaccaggat
    18061 gtggaactcg tgcggcttgg cgtagacatg gcgcagcagc agcttctcag tgagccagcc
    18121 gatcgccccc acggccaggg gggcgagcag cagcgtccac cagaagctcc agccccagcg
    18181 cgtcatctgg tagcaaaagt aagcacctat caggtagaac gcaccgtgcg cgaagttcac
    18241 gaagcgcagc agaccgaaga cgatggacag gcccacggcc aacaggaaat acagcatgcc
    18301 aatgccgatt ccgttgatgg tctgtagcag gtagatgctc atgattggct attagaaaaa
    18361 tagctgtcag cgctttgcta gaaagcgctg aatggccttt ttgcttaaag atggcggcgg
    18421 ggcgtcaggc cagcttgcag ccggtctggt ccacgggcag gaaggacttg ccggagctga
    18481 tcacatccac gtagtcatcc ttgttcttca tcttcgcctt ggccttgccc ttgagcaggt
    18541 agtagttttt gagcacctgg tggtccgcct tgcgcacctc ctcctcgccc gtcaggccct
    18601 ggtacttcat gccttcgagt gcggtgatga cggccttttg ctccaccgag ccggccttga
    18661 cgatggcgtc gatgagcagc ttggtgcaga tgtaggagcc cgccaggctg tagttggggt
    18721 tggccttgaa cttgtcctgg cagcgcttga ccagatccag gttttgtggg gcgtccgccg
    18781 tgtgccagta ctgggcgcca aagtacacgc cgtcgcacag gtcggcgccc agcgactcga
    18841 actgttccag ccccgaggcc caggcgatga gaatggtcat gttcttgtgc atgccgaagc
    18901 tgatggcctg gcgcagcgtg tcggacgact gggcgccaaa gttcaacaac agcaacacgt
    18961 cgggcttggc ggccacggcg ttggtgaggt agccgctgaa ctccttctcg gtcagcgagt
    19021 ggtagctgtt gcccacatgc tctatgccct tttccttgaa gatgttcttg gctgccgaca
    19081 gcaggccatc gccgaacaca tattgcggcg tgatcgtgta ccagcgcttg gccttcggca
    19141 tggcctctac cagtggacgc acggtctgct cgatggcacc gaaggtgggc acggaccagc
    19201 ggaaggtggc gctgttgcag tccttgccgg tgatctcgtc ggcgccggcc gtggtgatga
    19261 acacgccgcc ggccttctcg gcctccttgc ccatggccag cgcctcggac gacagaatgc
    19321 cgccggcaaa gaagcgtgcg ccctgctgct gtgccgattc ctgcaccttg cgcaccgccg
    19381 tcgccggctt gccctcggtg tccagcagcg tataggccag cggccggccc agcgccttgc
    19441 cgtactgctc tatggccagc ttcatgccca ggtcggcgaa cttgccgttg gctgcgaacg
    19501 ggcccgacat gggtaccggg cagccgaact ggatcgcgtc cttggcctgt gcccaggcag
    19561 tggatgccag cgacagcgag gagggcaggg cgccgagcgc ggcaagttgc agaaggtgac
    19621 gacgttgcat gatgtggtgt ctccgtggcg gtgtaggtgt agtgactggt tccggggccg
    19681 gtttcggagt gcgtgagcgc aatttcatgc ctgcaatgcg tacgaacccc ttaactccta
    19741 tttatagtga taaatacaat aaacattacg cgtgaaaacc cttgaactga ccatgaccaa
    19801 accgaagact tcctcattgg cgatcaaaca gctcaagcgc tcggatctcg tggcccagga
    19861 ggtcaagcgc ctgatcaccg agaaaaatct cagtcccggc gaccgcctgc cgcgtgaaat
    19921 cgagctgcag gagcagttcc aggtcagcaa gggcacgatc cgcgaggcgc tcaagtcgtt
    19981 ggaggtgcag gggttgatca ccatctccac cggtccgacg ggtggcggca ccatcgccga
    20041 ggtgccgctc gatcgcacgc tgcagttcat gcagaactac ctgttcttcc aggaggtgac
    20101 gatcgacgac atctacaccg tgcgccagat gctcgaaccc gagctggcgg caggcgccgt
    20161 gccgcacctg accgaggccg acttcgaggc gctggagcac agcatcagct gctgtgaccc
    20221 cacgcaaagc catgaggatt tgctcaccca gcggcgcgag gatgtgaact ttcacgacat
    20281 cctggccgcg gccaatccca accccttcct gcgcttttcc tgcgagctca tcaacgagat
    20341 gattcgccag ctcatcgtct tcggcaaccg cacgccgcag accgagcacc ggcgctttgg
    20401 cgaggccaac gcgcagttcc accgcgacat cgtgcaggcc gcccgcgcgc gtgacgccga
    20461 gcgggtgcgc gtgctgatgc accagcacat gcaggacgcc gccagcagcg tcaagcgcat
    20521 gaaggggcgc atccaggggc gactgatcct ggatgcggac acgctgcgcc ggcccaggcc
    20581 cgtggccggg cgcaagcgtg cctgaagtgg gctctgcgtc tcgcaagtcc atgggagctg
    20641 ataagctgcg ccgcgttttt caaccatcca acgaacccat gacccatcca ttca
//
LOCUS       DAOMTQ010000039        20668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_305_length_20668_cov_26.851113, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000039 DAOMTQ010000000
VERSION     DAOMTQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 20668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20668
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_305_length_20668_cov_26.851113"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /gene="rng"
                     /locus_tag="PK518_05775"
     CDS             complement(<1..1479)
                     /gene="rng"
                     /locus_tag="PK518_05775"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HRM48456.1"
                     /translation="MQQDILINWSPQETRVAVVENGAVQELHVERTLERGLVGNVYLG
                     KVSRVLPGMQSAFIDIGLERAAFLHVADVWQRQEGGEAPMFARKGEPPLPIEKQVFEG
                     QALMVQVIKDPIGSKGARLSTQISIAGRLLVFLPQDDHIGVSQKIPQDERDALRARLA
                     ALVGDKSTGGGGGFILRTNGEDASDEELAEDIAYLRKTWAGIRQAALSRPPTSLLYQD
                     LSLLQRVLRDLVGEQTQTIRLDSREQFALLAQFGREFMPQAAAKLQLYKGERPIFDLY
                     SIDEEIARALGRRVELKSGGYLIVDQTEALTTIDVNTGGYVGARNFDDTIFKTNLEAA
                     GAIARQLRLRNLGGIVIADFIDMLREDHQTAVLAEFKKQLARDRVKTMVGGFSQLGLV
                     EMTRKRTRESLAHMLCEPCPNCHGVGEVKTARTVCYDILRELLREARQFNPREFRVIA
                     SPQVVDLFLDEESQHLAGLSDFIGKPISLQAESTIGQEQYDIV"
     gene            complement(1557..2381)
                     /gene="cobA"
                     /locus_tag="PK518_05780"
     CDS             complement(1557..2381)
                     /gene="cobA"
                     /locus_tag="PK518_05780"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRM48457.1"
                     /translation="MSFIEDVRWFTAAPVTDTPDGPAPQGRVTLVGAGPGDPELLTLR
                     AVKALRGARLVLYDNLVGKEVLRYVAEDADLIYVGKEASRHTLPQESIIDLMVRLARS
                     GRSLVRLKGGDGFIFGRGGEEVLALAEAGIAFEVVPGITAAQGAGACAGIPLTHRDHA
                     ATLVFATGHLRGDNELALDWEALARPRQTVVIYMGVGNLPTICGELVRHGLPAQTPAA
                     LVEQASLPGQRCITGTLQDLPQLAQQHRVRAPALIVVGEVVGLQPQLMRGMQAAVL"
     gene            2558..2905
                     /locus_tag="PK518_05785"
     CDS             2558..2905
                     /locus_tag="PK518_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRM48458.1"
                     /translation="MNRTALLALSLMLTAAAAGAQTTAAPAPERTAQLVRMVRQDCGS
                     CHGMQLTGGLGPALTREALVAKPPEYLAAVITHGIPGTPMPPWSALLTPGEAQWIAQQ
                     LANGFPEERKQSR"
     gene            2902..4110
                     /locus_tag="PK518_05790"
     CDS             2902..4110
                     /locus_tag="PK518_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRM48459.1"
                     /translation="MKRRSLLSLTAGASLLAGGCAATKTPPATAATDTVQGTGDIGVV
                     IERAAGALTLVNTSRREAIGRVQGLGDLSHASVVFSRDARYAFVFGRDGGLTRVDMLQ
                     QRITHRVMQAGNSIGGAISADGRLVVAQNYQPGGIKVFDAATLELLADVPASMPGGER
                     SRVVGLADLPQHRFIYSLFDAEAICIADCSNPRQPRITTLQGIGRQPYDALLSPNGRH
                     YIAGLFGEDGLAMVDLWEEQPRARRILGGYGRGEKPLPVYKMPHLRGWAMADGRAFLP
                     AIGRHEVLVVDTSTWAEVGRIPVAGQPVFVMARPDGRQVWVNFSVPDYNRVQVIDTVS
                     QRVVQTIEPGKAVLHMEFTPRGESVWISCRDDNRVQVYDTHTRELQASLAVDAPSGIF
                     FTARAQRMGF"
     gene            4114..5145
                     /locus_tag="PK518_05795"
     CDS             4114..5145
                     /locus_tag="PK518_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM48460.1"
                     /translation="MQHKDLDLALLNPWQRDFPLQREPFAVIGQCLGLDVAQVLEHYT
                     RLQRQGSLSRIGAVFAPGAGGASLLAAMAVPPDRLNAVAAVVSSHPGVNHNYEREHTT
                     NLWFVATGRDAAQVEQLLRSIEAETDLPVLRLPMLRPYRIDTGFDLRASQSRASGATH
                     WAQTPLQAHDKPLAALAEQGLALVDRPYDLWAEQLGQGVDDVLAQLQRWLQQKTLSRF
                     GVVVRHHELGYTANAMTVFDVPESEVDACGEALAQMPGVTLAYQRARAGDWPYNLYCM
                     VHGRDRDSVRATVAQAVAHAGLAHRPHAMLFSLRRFKQTGARRFAAHTTQLAAHSCHT
                     PAGAPHAHP"
     gene            5132..5608
                     /locus_tag="PK518_05800"
     CDS             5132..5608
                     /locus_tag="PK518_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM48461.1"
                     /translation="MLTPDDARLINHLHGGFPLVDRPFAAVAEQLGWSEERVIERLHQ
                     LLAQGVLTRFGPLFQIERAGGQFVLAAIAVPEERFDAVNLVVNSFPEVAHNYRRTHAL
                     NMWFVVAAESPALAADAIARIQESVGLQVLAFPKEEEYFVELRLPALPTAGEAHGA"
     gene            5598..6077
                     /locus_tag="PK518_05805"
     CDS             5598..6077
                     /locus_tag="PK518_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM48462.1"
                     /translation="MALDAFDRALIAATQGGLPLVARPYEAVGAMLGASGEQVRARLT
                     QMLEHGLIRRIGAVPNHYRLGYTANGMSVWDVADEQIAELGPRVADLPGVSHCYRRPR
                     HLPGWPYNLFAMLHGTSREAVAQQAQEIVQLLGPACRSHDILYSTAILKKTGLRLKD"
     gene            6081..7295
                     /gene="nirJ"
                     /locus_tag="PK518_05810"
     CDS             6081..7295
                     /gene="nirJ"
                     /locus_tag="PK518_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HRM48463.1"
                     /translation="MFRISQYMRELAQAQSTGVYPVVKDRKARGPVVIWNLIRRCNLT
                     CKHCYALSADHDYAGELLLPEVFTVMDDLKAFGVPALILSGGEPLLRPDIFEISARAR
                     EMGFYTGLSTNGTLIDALMAQRIAEAGFDYVGISLDGLKATHDKFRRLEGAFDRSLAA
                     IGHLDKVGVKVGLRYTMTAMNGHDLPALLDLMQEVGAHKFYFSHLNYAGRGNIHRDKD
                     AHHQATRAAMDMLFDRAWAAAQAGSMDDYVSGNNDADGPYLLQWVRQHLPQWEDALRA
                     RLVAWGGNASGQQIANIDNLGHVHPDTMWWHHDLGCVRDRPFSQIWNDVSDPLMAGLK
                     QRPRPVQGRCGGCRHLAICNGNTRVRAQQLTGNFWFEDPGCYLSDAEVGAASTPFEPA
                     AQKRRRIEVAAA"
     gene            7497..8987
                     /locus_tag="PK518_05815"
     CDS             7497..8987
                     /locus_tag="PK518_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRM48464.1"
                     /translation="MLALALATPALAQPNAAALYQQHCASCHGAQRTGLMGPALLPES
                     LERTRPAEVLRVITEGRQATQMMGFAEQLSPPEIKALADWVRTPVVPAPRWGEADIRA
                     SRIATPLPADEPVKPKWDADPMNLFLVVEGGDHHVSLLDGDRFEVITRFASRYALHGG
                     PKFTPDGRYVFFGSRDGWITKYDLWRLAVVAEVRAGLNMRNVAVSGDGRWVMAANYLP
                     HSLALFDADLNLVTTYDAATQDGKSSSRVSAVYDATPRNSFVVALKDLPEIWEISYDQ
                     KTPATPKSQWGVRRTLLEEPLDDFFFDQSYRHALGATRPKAGGSASAQVVSLDMRRKV
                     ADLPIAGMPHLGSGITFAWNGTTVLASPNLGGGAVDVIDMQSWKTVRTIPTPGAGFFM
                     RSHENTPYAWTDSMMSKDGKDRLTIIDKRTLTPVAEVREPGKTLAHIEFTRDGRYALA
                     SLWEMDGAVIVYDARSFKEVKRLPMAKPVGKYNVWNKITRSEGTSH"
     gene            9137..9862
                     /locus_tag="PK518_05820"
     CDS             9137..9862
                     /locus_tag="PK518_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM48465.1"
                     /translation="MNIRQFNIPHYLSTLPAFQEMTPAELERLATGCRLRRYARGISI
                     YRVGMSCEEFHVAVKGQVKLYAISPAGQEKVVELAGPGMCFGEASMFSDQLYILNAEA
                     LTDSIILSVGKAAVVREIEADPRFAMHMLSGISRRMHALVQDVQAYALHSGMERVIGY
                     LLHSLPEEGTYHVTCNRPCNEAMALNVSLPVSKATIASRLSITPEYFSRVLHDLEEAG
                     LIRRDKRDIHIPDTARLASHTLQ"
     gene            complement(9874..10491)
                     /locus_tag="PK518_05825"
     CDS             complement(9874..10491)
                     /locus_tag="PK518_05825"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRM48466.1"
                     /translation="MTDFIYLASQSPRRRQLLAQLGVRHELLLPNAVGDAPEDAEGLE
                     AVQAGEAPAAYVQRVTALKLDAAVARHKRRGLPLAPILCSDTTVALGERIYGKPEDAQ
                     DAARMLAELSGREHQVLTAVALQAGDRRLAALSVSRVRFAAMTEAQIAAYVASGEPMG
                     KAGAYGIQGPVAQYVQNINGSYTGIMGLPLFETAQLLRAVGVLRD"
     gene            complement(10555..11022)
                     /gene="rlmH"
                     /locus_tag="PK518_05830"
     CDS             complement(10555..11022)
                     /gene="rlmH"
                     /locus_tag="PK518_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRM48467.1"
                     /translation="MRLLIVAVGQRVPDWAASAYDDYAKRFPPELKVELKAIKTEPRG
                     SKTLQTLYAAERERIEAAIPRGTRVVALDERGTSLTTKALAERLKGWQLGGDDVALVI
                     GGPDGLDPDFRQAAHERIRLSDLTLPHAMVRVLLIEQLYRAWSVNAGHPYHRE"
     gene            complement(11019..11804)
                     /gene="rsfS"
                     /locus_tag="PK518_05835"
     CDS             complement(11019..11804)
                     /gene="rsfS"
                     /locus_tag="PK518_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRM48468.1"
                     /translation="MTTSPQSETAAKKDVTKLQRAIVDGLEDVKAQDIQVFNTEHLSP
                     LFERVIVATGSSNRQTKALAASVRDAVREAGFVKPRTEGEENGEWIIVDCGQAVVHVM
                     QPAIRQYYRLEEIWGDKPVRMKLGAAKPRKIMASEDASGAPVRKPAAKKAAAKPVAKS
                     APAKAAAKKAPAKAAAKPASAKPAAKATGKTVAAKKPVAAKKPVAAKKPALKAAVKTV
                     VVNKPAAKQAPPKAAAKATARKPAAKAASTRPAAKKAPARKKA"
     gene            complement(11852..12802)
                     /gene="hemF"
                     /locus_tag="PK518_05840"
     CDS             complement(11852..12802)
                     /gene="hemF"
                     /locus_tag="PK518_05840"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRM48469.1"
                     /translation="MDQALNQPGHVAQVRAYLEGLQSRITAALEEIEGPAEQGGVRFR
                     SDAWRREPGSPLQGDGITRILEGGRVFERAGCGFSHVSGPSLPPSATQHRPELAGAPF
                     EAMGVSLVFHPRNPYVPTVHMNVRMIAAGLPGQAPTCWFGGGMDLTPYYGFEEDAVAF
                     HRACRDALAPFGDGLYPRFKTWCDEYFYLKHRNEQRGVGGIFFDDFSELGFAQSLAMT
                     QSVGDAFLGAYLPIVQRRQAMPHGAREREFQLYRRGRYVEFNLVWDRGTHFGLQSGGR
                     TESILLSMPPLAAWSYQREDAPGTPEADLTARFLQRRDWL"
     gene            complement(12802..14085)
                     /gene="purD"
                     /locus_tag="PK518_05845"
     CDS             complement(12802..14085)
                     /gene="purD"
                     /locus_tag="PK518_05845"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRM48470.1"
                     /translation="MKILVIGGGGREHALAWKLSQSPRTTKVYVAPGNGGTALSPRYE
                     NLPITDVVALREWAQTNKVALTVVGPEAPLAAGVVDEFRAHGLRIFGPTRAAAQLESS
                     KAFSKDFMKRHGIPTAAYDTFTDAAAAHAYVERLGAPIVIKADGLAAGKGVVVATSLQ
                     EAHDAVDFMLVDNKYGVAHNEGGARVVIEEFLEGEEASFIVLCDGKNVVALATSQDHK
                     RLKDGDQGPNTGGMGAYSPAPVVTADVHARAMREVILPTVRGMEKDGIPYTGFLYAGL
                     MIDAQGHPKTLEFNCRMGDPETQPILMRLKSDLVEVLGAAVDGRLDQVDLQWDRRTAL
                     GVVMAAHGYPDSPRKGDAITGLPQDEDDAMVFHAGTQLEGGVLRTSGGRVLCVTGLAD
                     NVKQAQQRVYDMARGIYFDGAQYRRDIGYRAVKGS"
     gene            complement(14104..14823)
                     /locus_tag="PK518_05850"
     CDS             complement(14104..14823)
                     /locus_tag="PK518_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM48471.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGKIWTRIIREITVAARQGGGDPA
                     ANPRLRLAIDKAKAANMPADRIKYNIDKASGTLEGINYEEIRYEGYGIGGAAIIVDTM
                     TDNRVRTVAEVRHAFSKYGGNMGTEGSVAFQFKHVGQLVFAPGTDEDKVMEVALEAGA
                     EDVITDDEGAIEVLTAPGDFEAVKNALEAAGLTAEVAEVTMRPENTIALEGDDAARMQ
                     KLLDIIEDLDDVQDVFHNAEL"
     gene            14895..16403
                     /locus_tag="PK518_05855"
     CDS             14895..16403
                     /locus_tag="PK518_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRM48472.1"
                     /translation="MADPLLIAQHDSTQCHLLPGLANRHGLITGATGTGKTVTLQTLA
                     EQFSRIGVPVFMADVKGDLTGISQKGSIGEKMAMALTERGLPTPEPVACPTTLWDVFG
                     EQGHPVRATISDMGPLLLGRMLNLNETQLGVLNLVFKIADDNGLLLLDLKDLRAMLQY
                     VGDNAKDFTTEYGNISSASVGAIQRGLLQIETQGGDQFFGEPMLNIQDFMQTVDGRGV
                     INVLAADKLMNSPRLYATFLLWMLSELFEQLPEIGDPEQPKLVFFFDEAHLLFNEAPK
                     VLVERIELVVRLVRSKGVGVYFVTQNPLDIPDSVLAQLGNRVQHALRAFTPRDQKAVK
                     ATATTMRPKAGLNIEAAITELAVGEALVSFLDPKGRPCETERVYVLPPGSQIGPISDT
                     QRRALLAGSLVAGTYDQSIDRESAYEKLRGRADAATNNAATPQAGSDTQGDGGLIGGL
                     NDVLFGSTGPRGGKKDGLVQTMAKSAVRTMGTSLGKEILRGVLGGIFGGRKR"
     gene            complement(16518..17222)
                     /locus_tag="PK518_05860"
     CDS             complement(16518..17222)
                     /locus_tag="PK518_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM48473.1"
                     /translation="MGAQWKAKGKALVADAKGKLFGKLVKEIIVAARAGADPAGNSRL
                     RLAVEAARKASMPKDTLERAIKKGSGVGSDAVNYERVIYEGFAPHQVPVMVECLTDNV
                     NRTAPEMRVCFRKGQMSAVAWDFDHVGMIEAEPEQSGADAEVAAIEAGAQDFEPGEEE
                     GQTLFLTDPTDLDAVAKALPGFGFKVLSAKLGYKARNPVSMAGLAPEAQEEVQAFLAG
                     LENNDDVQNVYVGLVD"
     gene            17398..18027
                     /gene="upp"
                     /locus_tag="PK518_05865"
     CDS             17398..18027
                     /gene="upp"
                     /locus_tag="PK518_05865"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRM48474.1"
                     /translation="MSNVHVITHPLVQHKLTLMRKKDASTNSFRRLLGELSTLMAYEV
                     TRDMPLQEVQIQTPLETMTAKVIDGKKLVLVSILRAGNGFLDGMLNVVPGARIGHIGL
                     YRDPTTLQPVEYYFKMPSEMAERDVIVVDPMLATGNSAAAAVERIKQLRPRSIKFVCL
                     LAAPEGVATLQKAHPDVPIFTAAIDRELNDHGYILPGLGDAGDRIFGTK"
     gene            18080..19492
                     /locus_tag="PK518_05870"
     CDS             18080..19492
                     /locus_tag="PK518_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRM48475.1"
                     /translation="MHLDRLSRRVARAGCATALVLAAHLPAAAEGAATTPLSTCASCD
                     TAALTDADIQTSRQVWHAPGSLPDKPMPVFQGVDMLNLFIVVESGSQHVTVLDGDRFE
                     PLHRFALHRALQGAPRFSPDGRFAYFLSSEGWVTQLDLWNLQAVAEVRAGQDSRSLAI
                     SGNGKYLAVANQRPHKLVLLDAELNLLKAHPALNQQRTRSSPILSVHDAAPRRSFVAV
                     LQDVPEIWEISYNPAADDVPIGMIHDFLYKEGAFIPGFLNPRRSVLPEPMGQVFFTPS
                     HHELLGMSRQGGKSQVIHLDVRKTIASLQLSDMPASGAAATWNYRPAPGTPERSVMAL
                     PSPGDGLVRLLDLESWATLKTVPMPGPGRLMRSHEGAPHVWMIQAPTSNGAGALQLLD
                     KHKLEHVASIHTDLGKDLAHVAFSRDGRYALASLRERKADGGAVIVFDTATLKEIKRI
                     PMDGPVGAYNLHNQIARPEP"
     gene            complement(19519..>20668)
                     /locus_tag="PK518_05875"
     CDS             complement(19519..>20668)
                     /locus_tag="PK518_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRM48476.1"
                     /translation="YRRLHWPPRRRSRRAYAVHISRVSASGRYLFVIGRDGRVNMIDM
                     WMETPANVAEIRIGLEARSVETSKFKGYEDKLAIAGAYWPPQFVIMNGDTLEPLKIVA
                     TRGMTEDTQEYHPEPRVASIVASLHRHEFIVNVKETGKTIMVDYSDMKALKLTEVGVA
                     RYLHDGGWDSTKRYFMVAANQSNKIGVIDSKTDTVAAVVDVGKIPHPGRGANFKHPKY
                     GPVWSTGHLGDETVSLIGTDPVKNKQHAFKVVQTLKGQGGGSLFLKSHPKSRHLYVDT
                     PLNPDAGLSQSIAVYDINQLDKGYKVLPIGQWAGLPEDGGAKRVVQPEYNRAGDEVWF
                     SVWSAKDKASAIVVVDDKTLQLKAVIKDPRLITPTGKFNMHNTQHDVY"
BASE COUNT         3498 a         7176 c         6511 g         3483 t
ORIGIN      
        1 aacaatgtcg tactgctcct gcccaatggt gctttccgcc tgcagcgaga tcggcttgcc
       61 gatgaagtcc gacaggcccg ccaggtgctg gctttcttca tcaagaaaca gatccaccac
      121 ctggggcgag gcgatcacgc ggaactcacg cgggttgaac tggcgcgcct cgcgcagcag
      181 ctcgcgcagg atgtcgtagc acacggtgcg cgcggtcttg acctcgccca cgccgtggca
      241 attcgggcag ggctcgcaca gcatatgggc cagcgactcg cgcgtgcgtt tgcgcgtcat
      301 ctccaccagg cccagctgtg aaaacccgcc caccatggtc ttgacgcggt cgcgcgccag
      361 ctgcttcttg aactcggcca gcacggccgt ctggtgatcc tcgcgcagca tgtcgatgaa
      421 gtcggcaatc acgatgccgc ccaggttgcg cagccgcaac tggcgcgcaa tcgcgcccgc
      481 cgcctccagg ttggtcttga agatggtgtc gtcaaagttg cgcgcaccga catagccgcc
      541 ggtattcaca tctatggtgg tcagtgcctc ggtctggtcc acgatcaggt agccgccgga
      601 cttcagttcc acgcgccggc ccagggcgcg ggcaatctcc tcgtcgatcg agtacaggtc
      661 gaagatgggc cgctcgccct tgtacagctg cagcttggcc gccgcctgcg gcatgaattc
      721 acggccgaac tgcgccagca gcgcaaactg ctcgcgtgaa tccaggcgta tggtctgcgt
      781 ctgctcgccc accaagtcac gcagcacgcg ctgcagcagg ctcaggtcct ggtacagcag
      841 cgaggtcggc gggcggctca gcgccgcctg gcggatgccg gcccaggtct tgcgcaggta
      901 ggcgatgtcc tcggccagtt cctcgtcgct cgcgtcctcg ccattggtgc gtaggatgaa
      961 gccgccacca ccgcccgtgg acttgtcgcc caccagtgcc gccaggcgcg cgcgcaaggc
     1021 gtcgcgctcg tcctgcggga tcttttgcga cacgccgata tggtcgtcct gcggcaggaa
     1081 caccagcagc ctgccggcaa tgctgatctg cgtggacagg cgcgccccct tgctgcctat
     1141 cgggtccttg atcacctgca ccatcagcgc ctggccctcg aagacctgct tttctatggg
     1201 caagggtggc tcgcccttgc gcgcgaacat cggcgcctca ccgccctcct ggcgctgcca
     1261 gacatcggcc acatgcagga aggcggcgcg ctccaggccg atgtcgatga aggccgactg
     1321 catgccgggc agcacgcgcg agaccttgcc cagatacacg ttgccgacca ggccgcgctc
     1381 cagggtgcgc tccacgtgca gctcctgcac ggcgccgttt tccaccaccg cgacgcgtgt
     1441 ctcctgcggt gaccagttga tcagaatgtc ttgttgcatg ggcttcgcca aatgaggcgc
     1501 ccatgatgcc tgaaacgcgc cctggccacg gcgccacaca ggccgactga agcgccttac
     1561 agcacggccg cctgcatgcc ccgcatcagc tgcggctgca gcccgaccac ctcacccacc
     1621 acgatcaacg caggcgcacg cacccgatgc tgctgcgcca gttgcggcaa atcctgcagc
     1681 gtgccggtga tgcaacgctg gccgggcagc gaggcctgct ccaccagggc agccggtgtc
     1741 tgtgccggca ggccatggcg caccagctcg ccgcagatgg tgggcagatt gcccacgccc
     1801 atgtagatca ccacggtctg gcgcgggcgt gccaacgcct cccagtccag ggcgagctcg
     1861 ttgtccccac gcagatggcc ggtggcaaac accagcgtgg cggcatggtc gcgatgcgtc
     1921 agcggaatgc ctgcgcaggc gcccgcaccc tgcgccgcgg tgatgccggg aaccacctcg
     1981 aaggcaatgc cggcctcggc cagggccagg acctcctcgc cgccgcgacc aaaaatgaac
     2041 ccgtctccgc ccttgagccg caccagcgaa cgcccgctgc gcgccagccg caccatcagg
     2101 tcgatgatgg actcctgcgg caaggtgtgg cgcgatgcct ccttgccaac gtagatcagg
     2161 tcggcgtctt ccgcgacgta gcgcagcacc tccttgccca ccaggttgtc atacagcacc
     2221 agtcgcgccc cacgcagcgc cttgacggcg cgcagcgtca gcagctccgg gtcgccgggg
     2281 ccggcgccga ccagtgtcac gcgcccctga ggggcaggcc catcgggcgt atccgtgacg
     2341 ggagcggctg tgaaccagcg cacatcttct atgaagctca tgtctgcctt tcctgggctg
     2401 atcgtggtgt gggctccatt gttcaaagag tgcaaaggat tcgtcgttga tcaaagtcaa
     2461 ggacagcctg cggcctcagc ggcgaccatg acgccattcg ctccggaaca tgcagtgcat
     2521 gcacatcaga gcacccgctc tcgacccagc ccaagccatg aacagaacag cactcctcgc
     2581 cctcagcctg atgctgaccg cagcggcagc cggcgcgcaa acaacagctg ccccggcgcc
     2641 ggaacgcacg gcgcagctgg tgcgcatggt gcgccaggac tgcggctcct gccatggcat
     2701 gcagctcacg ggcggtctgg gcccggcgct cacgcgcgaa gccctggtcg ccaagccccc
     2761 cgaatacctg gccgccgtca tcacccacgg cattcccggc acccccatgc caccctggag
     2821 cgccctgctc acgccgggcg aggcgcagtg gatagcccag caactggcca acggattccc
     2881 cgaagaaaga aagcagagtc gatgaaaagg cgttccctgc tgtcactcac cgctggcgcg
     2941 tcgctgctgg ccggtggttg cgcagccacc aagaccccgc ccgccaccgc cgcaaccgac
     3001 accgtccaag gcaccggtga catcggcgtg gtcatcgagc gcgccgctgg cgcgctcacc
     3061 ctggtcaaca ccagccggcg cgaggccatt gggcgcgtgc aaggcctggg cgacctgtcc
     3121 catgcctcgg tggtgttctc gcgcgatgcg cgctacgcct ttgtcttcgg ccgcgacggc
     3181 ggcctgacgc gcgtggacat gctgcagcag cgcatcacgc accgcgtcat gcaggccggc
     3241 aactcgatag gcggcgccat cagcgccgac ggccgtctgg tggtagcgca gaactaccag
     3301 cctggcggca tcaaggtgtt cgacgccgcg acgctggaat tgctggccga tgtgcccgcc
     3361 agcatgcccg gcggcgagcg ctcgcgcgtc gtggggttgg ccgacctgcc tcagcaccgc
     3421 ttcatttaca gcctgttcga cgccgaggca atctgcatcg ccgactgcag caacccgcgc
     3481 cagccacgca tcaccaccct gcagggtatt ggccgccagc cctacgacgc cctgctctcg
     3541 ccgaacggcc gccactacat cgccggcctg tttggcgagg atggcctggc catggtcgat
     3601 ctgtgggaag agcagccgcg cgcgcgccgc attcttggcg gctacgggcg cggcgaaaag
     3661 cccctgccgg tgtacaagat gccgcacctg cgcggctggg ccatggccga tggccgcgcc
     3721 ttcctgcccg ccattggccg gcacgaggtg ctcgtcgtcg ataccagcac ctgggccgag
     3781 gtgggccgca tcccggtggc cggccagccg gtattcgtca tggcacggcc cgatggacgc
     3841 caggtgtggg tcaacttctc ggtacccgac tacaaccgcg tgcaggtcat agacaccgtg
     3901 agccagcgcg tggtgcagac catagaaccc ggcaaggccg tgctgcacat ggagttcacc
     3961 ccgcgcggcg aatcggtgtg gatcagctgc cgcgacgaca accgggtgca ggtctacgac
     4021 acgcataccc gcgagctgca ggccagcctg gccgtggatg cgcccagcgg catcttcttc
     4081 acggcacgcg cacagcgcat ggggttctga tccatgcagc acaaggatct cgatctcgcc
     4141 ttgctcaacc cctggcaacg tgatttcccg ttgcagcgcg agcccttcgc cgtcataggc
     4201 cagtgcctcg gcctggacgt tgcccaggtg ctggagcatt acacccgcct gcagcgccag
     4261 ggcagcctga gccgcatcgg cgccgtgttt gcgcccggcg cgggaggggc ctcgctgctc
     4321 gccgccatgg cggtaccacc cgacaggctg aacgcggtgg ccgccgtcgt ctccagccac
     4381 ccgggcgtca accacaacta cgagcgcgaa catacgacca acctgtggtt cgtcgccacc
     4441 ggccgcgatg cggcgcaggt ggagcagttg ctgcgcagca tagaggccga aaccgacctg
     4501 ccggtgctgc gcctgcccat gctgcggccc taccgcatag acaccggctt tgacctgcgc
     4561 gccagccaat cgcgcgccag cggcgcaacc cactgggcac aaacaccgct gcaagcacat
     4621 gacaagcccc tggcggcact ggccgagcaa gggctggcgc tggtggacag gccctatgac
     4681 ctgtgggccg agcagctggg gcaaggcgtg gatgacgtac tggcgcagct gcagcgctgg
     4741 ttgcagcaaa agacgctgag ccgctttggc gtcgtggtgc gccaccacga gctgggctac
     4801 acggcgaacg cgatgacggt gttcgacgtg cccgaaagcg aggtcgatgc ctgtggcgag
     4861 gccctggcgc aaatgcctgg ggtgacgctc gcctaccaac gcgcacgcgc cggcgactgg
     4921 ccctacaacc tgtactgcat ggtgcatgga cgcgaccgcg acagcgtgcg tgccaccgtg
     4981 gcccaggccg tggcgcatgc cggcctggcg cacaggcccc atgccatgct tttctcgctg
     5041 cgccgcttca agcagaccgg tgccaggcgc tttgctgcgc acaccacaca gctggcagcg
     5101 cattcctgcc acacgccagc cggagcgccg catgctcacc cctgacgatg cccgtttgat
     5161 caaccacctg catggcggct ttccgctcgt cgatcgaccc tttgccgccg tggccgagca
     5221 gctgggctgg agcgaagagc gcgtcataga gcgcctgcac caactcttgg cccagggcgt
     5281 actcacgcgc ttcgggccgc tgttccagat cgaacgcgcg ggtggccagt tcgtgctcgc
     5341 tgccattgcc gtgcccgagg agcgctttga cgccgtcaac ctggtggtca acagctttcc
     5401 cgaagtggcg cacaactacc ggcgcacgca cgcgctcaac atgtggttcg tcgtcgccgc
     5461 cgaatcgccg gcgctggcgg ctgatgcgat cgcgcgtatc caggagtcgg tgggcctgca
     5521 ggtgctggcc ttccccaagg aagaagaata tttcgtcgag ctgcgcctgc cggcgctgcc
     5581 gaccgccgga gaagcccatg gcgcttgatg ccttcgaccg cgccctgatc gctgccacgc
     5641 aaggcggcct gccgctggtg gcgcgcccct acgaggccgt gggcgccatg ctgggcgcca
     5701 gcggcgagca ggtgcgcgca cgcctgacgc agatgctgga gcacggcctg atccggcgca
     5761 taggcgccgt gcccaaccac taccgcctgg gctacaccgc caacggcatg agcgtgtggg
     5821 acgtggccga cgaacagatc gccgaactgg ggccgcgcgt ggccgatctg ccgggcgtca
     5881 gccactgcta ccgccgcccg cgccacctgc ccggctggcc ctacaacctg tttgccatgc
     5941 tgcacggcac cagccgcgag gccgtcgcgc agcaggcgca ggagatcgtg caactgctgg
     6001 gccccgcctg ccgcagccac gacatcctgt attccacggc catcttgaag aaaaccggcc
     6061 tgcgtttgaa ggactgaaga atgtttcgca tcagccaata catgcgcgaa ctggcgcaag
     6121 cgcaaagcac cggcgtctac ccggtagtca aagaccgcaa ggcgcgcggc ccggtcgtga
     6181 tctggaacct gatccgccgc tgcaacctga cctgcaagca ctgctatgcc ctgtcggccg
     6241 accacgatta cgccggcgag ctgctgctgc ccgaggtgtt cacggtgatg gacgacctca
     6301 aggccttcgg cgtgccggcg ctgatcctct cgggcggcga gccactgttg cgccccgaca
     6361 tcttcgagat ctcggcgcgc gcgcgcgaga tgggcttcta caccggcctg tcgaccaacg
     6421 gcacgctgat cgacgcgctc atggcgcagc gcattgctga ggccggtttt gactatgtcg
     6481 gcatcagcct cgatggcctg aaggccacgc acgacaagtt caggcgcctg gagggcgcgt
     6541 ttgaccgcag tcttgccgcc atcggccacc tggacaaggt gggcgtcaag gtcggcctgc
     6601 gctacaccat gaccgccatg aacggccacg acctgccggc gctgctggat ttgatgcagg
     6661 aggtgggcgc gcacaagttc tacttctcgc acctgaacta cgccgggcgc ggcaacatcc
     6721 accgcgataa agacgcgcac caccaggcca cgcgcgcagc catggacatg ctgtttgacc
     6781 gcgcctgggc cgccgcgcag gccggcagca tggatgacta cgtgagcggc aacaacgacg
     6841 ccgacggccc ctacctgctg caatgggtgc gccagcacct gccgcagtgg gaggacgcct
     6901 tgcgcgcgcg cctggtcgcc tggggcggca acgccagcgg ccagcagatc gccaacatcg
     6961 acaacctcgg ccatgtccac cccgacacca tgtggtggca ccacgacctg ggctgcgtgc
     7021 gcgatcgccc cttctcgcag atctggaacg acgtgagcga cccgctgatg gccggcctga
     7081 aacagcgccc gcgcccggtc cagggccgct gcggtggctg ccggcacctg gccatctgca
     7141 acggcaacac ccgcgtgcgc gcgcagcagc tcaccggcaa cttctggttt gaagacccgg
     7201 gctgctacct gagcgacgcc gaggtggggg cggcatccac cccgtttgag cccgctgcgc
     7261 aaaagcgccg ccgcatcgag gtggcggcag cctaaacgcc ttcacctccc tctccccttt
     7321 gggggagagg gctggggtga gggcggatac cggcgcagcg tcccattttg gccccctcac
     7381 ccctcccctc tcccgccagc gggagagggt gaaatgccgg gtaacgccca ccattaaatc
     7441 gcgagtagct atattttttg gagcatcctt tgtcccacca catcttcctc agcgccctgc
     7501 tcgccctggc cttggccacc cccgccctgg cccagcccaa cgccgccgcc ctctaccagc
     7561 agcactgcgc cagctgccac ggcgcgcagc gcaccggcct gatgggcccg gcgctgctgc
     7621 ccgagagcct ggagcgcaca cgcccagcag aggtgctgcg cgtcatcacc gagggccgtc
     7681 aggcaacgca gatgatgggc tttgccgagc agctctcgcc ccccgagatc aaggccctgg
     7741 ccgactgggt gcgcacgccc gtggtgccgg cgccgcgctg gggcgaggcc gatatccggg
     7801 cctcgcgcat cgccacgccg ctgcctgccg acgagcccgt caagccgaag tgggacgccg
     7861 acccgatgaa cctgtttctc gtggtcgagg ggggcgacca tcatgtctcg ctgctcgacg
     7921 gcgaccgctt cgaggtcatc acccgctttg ccagccgcta cgcgctgcac ggcgggccga
     7981 agttcacgcc cgacgggcgc tatgtgttct tcggctcgcg cgacggctgg atcaccaagt
     8041 acgacctgtg gcgcctggcc gtggtggccg aggtgcgcgc cggcctgaac atgcgcaacg
     8101 tggccgtgag cggcgacggg cgctgggtca tggcggccaa ctacctgccg cacagcctgg
     8161 cgctgttcga cgccgatctg aacctggtga caacctacga cgcggcgacg caggacggca
     8221 agagcagctc gcgcgtgtcc gcggtgtacg acgccacgcc gaggaacagc tttgtcgtgg
     8281 cgctcaagga tcttcccgag atctgggaga tctcctacga ccagaagacc cccgccaccc
     8341 caaaaagcca atggggcgta cgccgtaccc tgctcgaaga gccgctcgat gatttcttct
     8401 ttgaccagag ctaccgccac gcgctgggcg ccacccgccc caaggccggg ggcagcgcca
     8461 gcgcccaggt ggtgagcctg gacatgcgcc gcaaggtcgc cgacctgccc attgccggca
     8521 tgccgcacct gggctcaggc atcacctttg cctggaacgg caccacggtg ctggccagcc
     8581 cgaatctggg cggcggcgcg gtcgatgtga tcgacatgca gagctggaag accgtacgca
     8641 ccatccccac gccgggtgcc ggcttcttca tgcgcagcca tgaaaacacc ccctacgcct
     8701 ggaccgactc catgatgagc aaggacggca aggacaggct caccatcatc gacaagcgca
     8761 cgctgacacc ggtagccgaa gtgcgcgaac ccggcaagac gctggcgcat atcgagttca
     8821 cgcgcgacgg ccgctacgcc ctggccagcc tgtgggagat ggatggcgcc gtcatcgtgt
     8881 acgacgcacg cagtttcaag gaggtcaagc gcctgcccat ggccaagccc gtgggcaagt
     8941 acaacgtctg gaacaagatc acgcgcagcg aaggcacatc ccattgaacg gatcgccgac
     9001 taggctgccc agatcacagg cattcatgcg cccctgagcg catggatgcc agccaattgg
     9061 cagaagaatt gacacatatc agggaaacgc tgtcctgctg tatctcgccc tagggtttac
     9121 aagaacaata cgccccatga atattcggca attcaacatc ccgcactacc tgtccacgct
     9181 gcccgcgttc caggaaatga cgcctgccga gctggagcgc ctggccaccg gatgccggct
     9241 gcgccgttat gcgcggggga tcagcattta ccgcgtgggc atgtcctgcg aggagttcca
     9301 cgtagccgtg aagggccagg tcaagctgta cgccatttcc cccgccggcc aggaaaaggt
     9361 ggttgagctg gccggccccg gcatgtgctt tggagaggcg tcgatgttca gcgaccagct
     9421 ctatatcctg aatgccgagg ccttgaccga ctccatcatc ctcagcgtcg gcaaggctgc
     9481 ggtggtgcgt gaaatcgaag ccgacccgcg ctttgccatg cacatgctga gcggtatttc
     9541 aaggcgcatg cacgccctgg tgcaggatgt gcaagcctat gcgctgcact ccggcatgga
     9601 gcgcgtgatc ggctacctcc tgcactcgct gcccgaagag ggcacatacc acgtcacctg
     9661 caaccggcca tgcaacgagg ccatggcgct caacgtgtcg ctaccggtca gcaaggccac
     9721 cattgcctcc aggctgtcga tcacgcccga gtatttctcg cgcgtgctgc acgacctgga
     9781 agaagcgggc ctgattcgcc gcgacaagcg cgacatccat atccccgata ccgcgcggct
     9841 ggccagccac acgctgcaat aggcccccgg cgctcaatcg cgcagcacac ccacagcccg
     9901 tagcagctgc gcggtttcaa acagcggcag tcccatgatg ccggtgtagc tgccgttgat
     9961 gttttgcaca tactgcgcca ccggcccctg tatgccgtag gcgccggcct tgcccatggg
    10021 ttcgccactg gcaacatagg cggcaatctg cgcctccgtc atggcggcaa agcgtacccg
    10081 cgacacggac aatgccgcca ggcggcgatc gcccgcctgc agcgccacgg cggtgagcac
    10141 ctggtgctcg cggccggaca gctccgccag catgcgcgcc gcatcctgcg cgtcctcggg
    10201 cttgccatag atgcgctcgc ccagggcgac cgtggtgtca gagcacagaa tcggcgccag
    10261 cggcaggcca cggcgcttgt ggcgcgcgac ggcggcgtcc agcttgaggg cggtgacgcg
    10321 ctgcacgtag gccgctgggg cttcgccggc ttgtaccgcc tccagccctt cagcatcctc
    10381 cggcgcgtca cccacggcat tgggcagcag cagttcgtgg cgcaccccca gctgggccag
    10441 cagctggcgc cggcgcgggc tttgcgaggc gaggtagatg aagtccgtca tgcgttaaac
    10501 aaaaaaggac gctagcgccc atccataaag cgtcaatagc tattaaaaat atagttattc
    10561 gcgatgatag gggtggccgg cattcaccga ccaggcgcgg tacagctgct cgatcagcag
    10621 cacgcgcacc atggcatgcg gcaaggtgag gtcggacagg cggatgcgct catgcgcggc
    10681 ctggcgaaag tccggatcca gcccgtcggg cccgccgatg accagggcca catcgtcgcc
    10741 gcccagctgc cagcccttga ggcgctcggc cagggccttg gtggtcaggc tggtgccgcg
    10801 ctcgtccagg gccacgacgc gcgtgccgcg cggaatggcg gcttcgatac gctcgcgctc
    10861 ggcggcatac agcgtctgca gcgtcttgga gccgcgtggc tcggtcttga tggccttgag
    10921 ctccaccttc agctcgggtg gaaagcgttt ggcgtagtcg tcgtaggcgc tcgcggccca
    10981 atcgggcacg cgctggccca cggccacgat cagcagcctc atgccttctt gcgtgcgggt
    11041 gccttcttgg ctgcaggcct ggtgcttgct gccttggcgg caggcttgcg cgccgtggcc
    11101 ttggcggcgg ccttgggcgg cgcctgcttg gccgcgggct tgttgacgac cacggtcttc
    11161 actgccgcct tgagcgccgg cttcttggcc gccaccggtt tcttggccgc cactggcttc
    11221 ttcgcggcca cggtcttgcc ggtggccttg gctgcgggct tggcgctggc gggcttggct
    11281 gcggccttgg ccggtgcctt cttggccgct gccttggccg gcgcactctt cgccacaggc
    11341 ttggcggcag ccttctttgc cgccggcttg cgcacgggtg caccgctcgc gtcttccgag
    11401 gccatgatct tgcgcggctt ggccgcaccc agcttcatgc gcacgggctt gtcgccccag
    11461 atctcttcca ggcggtagta ctggcggatg gccggctgca tcacatgcac cacggcctgg
    11521 ccgcagtcca cgatgatcca ttcgccgttt tcctcgccct cggtgcgggg cttgacgaag
    11581 ccggcctcgc gcacggcatc acgcacgctg gcggccaggg ccttggtctg acggttggag
    11641 ctgccggtag cgacgatgac gcgctcaaac aacggcgaga gatgttcggt gttgaagacc
    11701 tggatgtctt gcgccttgac gtcctccagt ccatcgacga tggcgcgctg gagcttggtg
    11761 acgtctttct tggcggcggt ttccgattgg ggggaagtgg tcatcagggg gtcttcaata
    11821 aaaagatggg ggcaatatag cgcgcggggg ttcaaagcca gtcgcggcgt tgcaaaaagc
    11881 gtgcggtaag gtcagcctcg ggcgtgcccg gcgcgtcctc gcgctggtag ctccaggcgg
    11941 ccaacggcgg catggacagc agaatggatt cggtgcgccc gcccgactgc aggccgaaat
    12001 gcgtgccgcg atcccacacc agattgaact ccacgtagcg gccgcggcga tagagctgga
    12061 attcgcgctc gcgcgcgcca tggggcatgg cctggcgccg ctgcacgatg ggcaggtagg
    12121 cacccaggaa ggcgtcaccc acgctctgcg tcatggccag gctttgcgca aagccgagct
    12181 cggaaaagtc atcgaagaag ataccgccca cgccgcgctg ctcattgcgg tgcttcaggt
    12241 agaaatactc gtcgcaccag gtcttgaaac gcggatacag cccgtcgcca aacggcgcca
    12301 gcgcatcacg gcaggcgcgg tgaaaggcca ccgcgtcctc ctcgaagccg tagtacggtg
    12361 tcagatccat gccgccgccg aaccagcagg tgggcgcctg cccgggcagg ccggcggcga
    12421 tcatgcgcac gttcatgtgc accgtgggca catagggatt gcgcggatgg aacaccagcg
    12481 acacgcccat ggcctcgaag ggcgcacctg ccagctcggg ccggtgctgc gtggccgacg
    12541 gcggcaggct ggggccgctg acatgcgaaa aaccacagcc ggcgcgctcg aatacacgcc
    12601 caccctccag gatgcgcgtg atgccgtcac cctgcagggg cgagcctggt tcacgcctcc
    12661 aggcatcgct gcgaaagcgc accccgccct gctctgccgg gccttcgatc tcctcgagcg
    12721 ccgctgtgat gcgtgactgc aggccttcca ggtaggcgcg cacctgggcc acatggcccg
    12781 gctggttcaa tgcctgatcc atcaactgcc cttcacggcg cggtagccaa tgtcgcggcg
    12841 gtactgcgcg ccatcgaaat agatgccgcg cgccatgtcg tacacgcgct gctgcgcctg
    12901 cttgacgttg tcggccagcc ccgtcacgca caacacgcgg ccgccgctgg tgcgcagcac
    12961 gccgccctcc agctgcgtgc cggcgtggaa caccatggca tcgtcctcat cctggggcag
    13021 gccggtgatg gcgtcgccct tgcgcgggct gtcggggtag ccatgcgcgg ccatgaccac
    13081 gcccagcgcc gtgcgcctgt cccattgcag atcgacctgg tcgagcctgc cgtccacggc
    13141 cgcgcccagc acctcgacca gatcgctctt caggcgcatc aggatgggct gggtctcggg
    13201 gtcgcccatg cggcagttga actccagcgt ctttgggtga ccctgggcgt cgatcatcag
    13261 gccggcgtac agaaacccgg tgtagggaat gccgtccttc tccatgccgc gcacggtggg
    13321 caggatgacc tcgcgcatgg cgcgcgcatg cacgtcggcc gttaccaccg gcgcgggcga
    13381 atacgccccc atgccgccgg tgttggggcc ctggtcgccg tccttcaggc gcttgtgatc
    13441 ctggctggtg gccagcgcga cgacgttctt gccgtcgcac agcacgatga aactggcctc
    13501 ttcaccctcg aggaattcct cgatcaccac gcgcgccccg ccctcgttgt gcgccacgcc
    13561 gtacttgttg tccaccagca tgaagtccac ggcgtcgtgc gcctcttgca ggctggtggc
    13621 cacgaccacg cccttgcccg ccgccaggcc gtcggccttg atgacgatgg gcgcgcccag
    13681 gcgctctaca taggcgtgcg ccgccgccgc gtcggtgaag gtgtcgtagg ccgccgtcgg
    13741 aatgccgtgg cgcttcatga agtccttgga aaaggccttg gagctttcca gttgcgcggc
    13801 cgcccttgtc ggcccgaaga tgcgcaggcc atgggcacgg aattcatcca ccacgccggc
    13861 ggccagcggt gcctcggggc cgaccacggt gagcgcgacc ttgttcgtct gcgcccattc
    13921 gcgcagtgcc accacatcgg tgatgggcag gttttcatac cggggcgaca gcgccgtgcc
    13981 gccgttgccc ggcgccacat agaccttggt ggtcctgggg gactggctga gcttccaggc
    14041 cagtgcgtgt tcccggccgc cgccgccaat gactaggatt ttcatagctt gtagttctcg
    14101 cgcctagagc tcggcgttgt gaaagacatc ttgcacatcg tccaggtctt cgatgatgtc
    14161 cagcagcttt tgcatgcgcg ccgcgtcgtc accttcaagg gcgatggtgt tctcgggacg
    14221 catggtgacc tcggccacct cggccgtcag gcccgcggcc tccagggcat tcttcacggc
    14281 ctcgaagtcg ccgggcgccg tgagcacctc gatggcgccc tcgtcgtcgg tgatcacgtc
    14341 ctcggcgccg gcctccagag ccacttccat gaccttgtcc tcatccgtgc ccggggcaaa
    14401 gacgagctgg cccacatgct tgaactggaa ggccaccgaa ccctcggtgc ccatgttgcc
    14461 gccgtacttg ctgaaggcgt ggcgcacctc ggccacggtg cgtacacggt tgtcggtcat
    14521 ggtgtcgacg atgatggccg cgccgcctat gccatagccc tcgtagcgga tttcctcgta
    14581 attgatgcct tcgagggtgc cagaggcctt gtcgatgttg tacttgatgc ggtcggccgg
    14641 catgttggcg gccttggcct tgtcgatggc caggcgcagg cgcgggttgg ctgccgggtc
    14701 gcccccaccc tgacgggcgg cgaccgtgat ttcacgaatg atgcgggtcc agatcttgcc
    14761 gcgcttttcg tcctggcggc ccttccgatg ctggatattg gcccatttac tgtgtcctgc
    14821 cactgcggcg tcctttgagt gatttaatct tgcggccggg attttacttt tgactcaccc
    14881 accatataac agccatggcc gatccgctcc tgattgcaca gcatgattcc acccaatgcc
    14941 acctgctgcc gggcctggcc aaccgccacg gcctgatcac cggggccacc ggcaccggca
    15001 agacggtcac gctgcaaacc ctggccgagc agttctcgcg catcggcgtg ccggtgttca
    15061 tggccgacgt gaagggtgac ctcacgggca tcagccagaa gggcagcatt ggcgaaaaga
    15121 tggccatggc gctgaccgaa cgcggcctgc ccacgccgga gcccgtcgcc tgccccacca
    15181 cgctgtggga tgtgttcggc gaacagggcc acccggtgcg cgccaccatc tccgacatgg
    15241 gcccgctgct gctggggcgc atgctcaatc tcaacgagac ccagctgggc gtgctcaacc
    15301 tggtgttcaa gattgccgac gacaacggcc tgctgctgct ggacctgaaa gacctgcgcg
    15361 ccatgctgca gtatgtgggc gacaacgcca aggacttcac caccgagtac ggcaacatca
    15421 gcagcgccag cgtgggcgcc atacaacgcg gcctgctgca gatcgaaacg cagggcggcg
    15481 accagttttt tggcgagccc atgctcaaca tccaggactt catgcagacc gtggatgggc
    15541 gcggcgtgat caacgtgctg gcggccgaca agctcatgaa ctcgccgcgc ctctatgcca
    15601 catttttgct gtggatgctg tccgagctgt tcgagcagct gcccgagatc ggtgacccgg
    15661 agcagcccaa gctggtgttc ttcttcgacg aggcccacct gctgttcaac gaggcgccca
    15721 aggtgctcgt cgaacgcatc gagctggtgg tacgcctggt gcgctccaag ggcgtggggg
    15781 tgtatttcgt cacgcagaac ccactggaca ttcccgacag cgtgctggcc cagctgggca
    15841 accgcgtgca gcacgcgctc cgggcgttta cgccacgcga ccagaaggcc gtgaaggcca
    15901 cggccaccac catgcgcccc aaggcggggc tgaacatcga ggccgccatc accgagctgg
    15961 ccgtgggcga ggccctggtg agctttctcg accccaaggg ccgcccctgc gagaccgagc
    16021 gcgtctacgt gctgccgccg ggcagccaga tcggccccat ctcggacacg cagcgccgcg
    16081 cgttgctcgc cggctcgctc gtcgctggca cctacgacca gagcatcgac cgcgagtcgg
    16141 cctacgagaa gctgcgcggg cgcgccgatg ccgctaccaa caacgcggcc acgccccagg
    16201 ctggctcgga cacgcagggc gacggcgggc tgatcggcgg gctcaacgac gtgctgtttg
    16261 gcagcaccgg cccacgcggc ggcaaaaagg acggcctggt gcagaccatg gccaaatccg
    16321 ccgtgcgcac catgggcacc agcctgggca aggagatact gcgcggcgtg ctgggcggca
    16381 tttttggtgg caggaagcgc tgaaacccca agccaaaacg gcctgcagcg cccatgaata
    16441 aagcgccagc agctattatt tatatagcta caggcgcttg tttcaagccc gtctgcgggc
    16501 catgcgaccg acgggtttca gtccaccagg ccgacgtaca cgttctgcac gtcgtcgttg
    16561 ttctctaggc ccgccaggaa ggcctgcacc tcttcctgcg cctcgggcgc caggccggcc
    16621 atgctgaccg ggttcctggc cttgtagccg agcttggccg acagcacctt gaagccaaaa
    16681 ccgggcagcg ccttggccac cgcgtccagg tcggtcgggt cggtcaggaa cagggtctgg
    16741 ccctcctcct cgcccggctc gaaatcctgg gcgccggcct ctatggcggc cacctcggca
    16801 tcggcgccgg actgctcggg ctcggcctcg atcatgccca catggtcgaa gtcccaggcc
    16861 acggcgctca tctggccctt gcgaaagcac acgcgcatct cgggcgccgt gcgattcacg
    16921 ttgtcggtca ggcactccac catcactggc acctggtgcg gggcaaagcc ttcgtagatc
    16981 acacgctcgt agttcacggc gtcgctgccc acgccggagc ccttcttgat ggcgcgctcg
    17041 agtgtgtcct tgggcatgga ggccttgcgc gccgcctcga ccgccaggcg caggcgcgaa
    17101 ttaccggccg ggtcggcacc cgcgcgtgcg gccacgatga tttccttgac cagcttgcca
    17161 aaaagcttgc ccttggcatc ggcgaccagc gccttgcctt ttgccttcca ttgcgcgccc
    17221 atagctgtaa attccttggt tgaatgccca caaaaaaagc ggcctgcgcc gcagcgggcg
    17281 agtgtaaacc cgccttgcac ggcggcatgc cgggtgaaat caactgcggc ccacacggct
    17341 gcggcaaaat ttccgctttc tgatcctcat ttctcaattt ccaagaggca gccgctcatg
    17401 agcaacgtcc acgtcatcac ccaccccctg gtgcagcaca agctgaccct gatgcgcaag
    17461 aaggacgcca gcaccaacag ctttcgccgc ctgctgggcg aactctccac gctgatggcc
    17521 tacgaggtca cgcgcgacat gccactgcaa gaagtgcaga tccagacccc gctggagacc
    17581 atgaccgcca aggtgatcga tggcaagaag ctggtgctgg tctccatcct gcgcgccggc
    17641 aacggctttc tggacggcat gctcaacgtg gtgcccggcg cgcgcatagg ccatatcggc
    17701 ctgtaccgcg acccgacgac gctgcagcct gtggagtact acttcaagat gccgtccgag
    17761 atggccgagc gcgacgtcat cgtcgtcgat cccatgctcg ccaccggcaa ctcggccgcc
    17821 gcggccgtgg agcgcatcaa gcaactgcgg ccgcgctcca tcaagttcgt ctgcctgctg
    17881 gccgcgccgg agggcgtggc cacgctgcaa aaggcccacc ccgatgtgcc catcttcacc
    17941 gcagccatcg accgcgagct caatgaccat ggctacatac tgcccggcct gggcgacgcg
    18001 ggcgatcgca tctttggcac caagtaagac aacgccaccg gcatggcgcc cgccatggat
    18061 ctttgccagg gtctttgcaa tgcaccttga tcgcctttcc cgccgcgttg cacgggcagg
    18121 ctgcgccacg gccctggtgc tggcggccca cctgccggcc gccgccgaag gcgctgccac
    18181 aacgcctctg tccacttgcg ccagctgcga cacggcggcg ctgacggacg ccgacatcca
    18241 aacctcgcgc caggtatggc atgcgccggg cagcctgccg gacaaaccca tgccggtctt
    18301 ccagggcgtg gacatgttga acctgttcat cgtggtcgaa tccggcagcc agcatgtcac
    18361 ggtgctcgat ggcgatcgat tcgagcctct gcaccgtttt gcactgcacc gggctctgca
    18421 aggggcgccc cggttcagcc ccgacggacg ctttgcgtac ttcctgtcgt ccgagggctg
    18481 ggtcacccaa ctcgatctct ggaacctgca agccgtggcc gaggtgcggg ccggccagga
    18541 cagccgcagc ctggccatat ccggcaatgg caaatacctg gccgtggcca accagcgacc
    18601 gcacaagctg gtgctgctcg acgccgagct gaacctgctc aaggcgcatc cggccctgaa
    18661 ccaacagcgc acgcgaagct cgcccatcct gtccgtgcac gatgccgcac cacgcaggag
    18721 ctttgtcgcg gtgctccagg atgtgcccga gatctgggag atcagctaca accctgctgc
    18781 agacgacgtg ccgataggca tgatccatga cttcctctac aaggagggcg cgttcatacc
    18841 agggttcctc aacccgcgcc gcagcgtcct gcccgagccc atgggccagg tcttcttcac
    18901 gccaagccac cacgagctgc tgggcatgag ccgccagggc ggcaagagcc aggtcattca
    18961 tctggacgtg cgcaagacga tcgccagcct gcagctgtcc gacatgccag catccggcgc
    19021 tgctgccacc tggaactacc gtccggcgcc tggcacaccg gagcgcagcg tgatggccct
    19081 gcccagcccc ggcgatggcc tggtcaggtt gcttgatctg gaaagctggg ccacgctcaa
    19141 aaccgtcccc atgccggggc caggccggct catgcgcagc cacgaaggcg caccccatgt
    19201 ctggatgatc caggcaccca ccagcaacgg cgcaggcgcc ctgcaactgc tggacaagca
    19261 caagctggag catgtggcca gcatccacac cgacctcggc aaggatctgg cgcatgtggc
    19321 cttcagccgc gacggccgat atgcgctggc cagcctgcgc gagcgcaagg ccgatggcgg
    19381 cgcggtgatc gtctttgaca ccgccaccct caaggagatc aagcgcatcc ccatggacgg
    19441 gcccgtcggg gcgtacaacc tgcacaacca gatcgcacgc cccgagccct gaataccgac
    19501 gcgcggtagg gaggtcggtc agtacacgtc gtgctgcgtg ttgtgcatgt tgaacttgcc
    19561 ggtcggcgtg atcaggcgcg gatccttgat cacggccttg agctgcaagg tcttgtcatc
    19621 caccacgaca atggccgagg ccttgtcctt ggccgaccag acagagaacc acacctcgtc
    19681 gcccgccctg ttgtactcgg gctgtacgac gcgcttggcg ccgccatctt cgggcaagcc
    19741 ggcccactgg ccgatcggca gaaccttgta gcctttgtcc agttggttga tgtcatacac
    19801 cgcaatcgac tgggaaagcc cggcatccgg gttcaacggc gtatccacat acaaatgcct
    19861 ggacttgggg tggctcttga ggaacagtga cccgccgccc tgccccttga gcgtctgcac
    19921 caccttgaag gcatgctgct tgttcttgac cggatcggta cctatcaggg acacggtttc
    19981 atctcccaga tggcccgtgg accataccgg gccgtacttg gggtgcttga agttggcacc
    20041 acgcccgggg tgcggaatct tgcccacatc gaccaccgcc gccacggtat cggtcttgga
    20101 gtcgatgacg ccaatcttgt tgctctggtt ggcggccacc atgaagtagc gcttggtcga
    20161 atcccaaccc ccatcgtgca ggtagcgcgc cacccccact tcggtgagct tcagcgcctt
    20221 catgtcggaa tagtcgacca tgatggtctt gcccgtttcc ttgacgttca cgatgaactc
    20281 gtgcctgtgc agcgacgcca cgatggaggc cacgcgcggc tcggggtggt attcctgcgt
    20341 gtcctcggtc atgccgcggg tggcgacaat tttcaagggc tccagcgtgt cgccattcat
    20401 gatcacgaac tggggcggcc aataggcccc ggcaatggcc agcttgtcct cgtagccctt
    20461 gaacttcgag gtctcgaccg agcgcgcctc caggccgatg cggatctcgg ccacattggc
    20521 cggcgtttcc atccacatgt cgatcatgtt cacgcgacca tcacggccga tgacgaacag
    20581 ataacgcccg gacgcggaca cacgagaaat atgcaccgcg tacgcccttc tgctcctgcg
    20641 ccgaggcggc cagtgcaatc gtcgatag
//
LOCUS       DAOMTQ010000040        20365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_316_length_20365_cov_26.700542, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000040 DAOMTQ010000000
VERSION     DAOMTQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 20365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20365
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_20365_cov_26.700542"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK518_05880"
     CDS             <1..456
                     /locus_tag="PK518_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRM48477.1"
                     /translation="AATLARLRARLERPTPRVALGLALRGLASSAIDVSDGLLGDLGH
                     VLKSSRVGAEIDVHITTDLLASGALQENAKGIFDAETMMPCALAGGDDYELVFTAPAA
                     RRAAVQAASSASGTQVTRIGRITPAPGLRLLDRTGQPLPGRFASFDHFA"
     gene            538..996
                     /locus_tag="PK518_05885"
     CDS             538..996
                     /locus_tag="PK518_05885"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRM48478.1"
                     /translation="MVCMGNICRSPTAEGVLRALVQRAGLQRRITVDSAGTHGFHVGV
                     APDKRSQQHAARRGYDLSRLRARQLTARDFERFDLVLVMDEANEQAALALCPPAHAGR
                     VHRLTQFCLRHQANAVPDPYYGGAQGFEQVLDLVEDACAGLLAALQARQP"
     gene            1015..1533
                     /locus_tag="PK518_05890"
     CDS             1015..1533
                     /locus_tag="PK518_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRM48479.1"
                     /translation="MNPAAAPRRPTPAFLLAHPAHCIALGFGSGLSPKAPGTVGTAWG
                     WLAFLVLELWLTPLGMGLVIGFSLLLGWWACTVTARHMGVADPGSIVWDEIVAIWIVL
                     WLAMPMGFWGQLAAFALFRYFDAAKPGPVAWADRLFKGFGWRGGWGILFDDLVAAGCT
                     LLVLALWRHLFG"
     gene            1539..2054
                     /locus_tag="PK518_05895"
     CDS             1539..2054
                     /locus_tag="PK518_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRM48480.1"
                     /translation="MTLSKHELSALDDKALQADLTHISSSLLAHGWMLATAESCTGGL
                     IAAACTDLAGSSQWFERGFVTYSNAAKVQCLGVPADLIERHGAVSEAVARAMAQGAVA
                     HSAAQVALAVTGVAGPTGGSADKPVGTVWFGWQVQGATHSELRRFAGDRAAVRAQTVR
                     HALARLARLLG"
     gene            complement(2070..2894)
                     /locus_tag="PK518_05900"
     CDS             complement(2070..2894)
                     /locus_tag="PK518_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HRM48481.1"
                     /translation="MTTIDNNRAAPDPALGPDALDELDLILEDLRTREDEVPQWEFCD
                     GFLTALVCTRRAISVAEWLPMLVGDGLELGLDAAGALPLMPPFKDLAQQQRFVHWCQL
                     RSAEVAAQLDAQAESLDADDAFQPEVLDMRGAIASLPEDERAEMEGQPIPSFGQIWAL
                     GFMFAVENWDDEWQAPRDKEAAQWLDDALQAIVALAEDDTGKPTICMYDEDGPPSTSQ
                     QRLEDFGEAIWAVYDLRQLWRSLGPRVQAIVHGPQPGRNDPCPCGSGKKHKKCCGA"
     gene            complement(3342..3824)
                     /locus_tag="PK518_05905"
     CDS             complement(3342..3824)
                     /locus_tag="PK518_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRM48482.1"
                     /translation="MHPELRLPARTQAGEGARAVVLDTNIVLDLFIFADPAVAPLRGL
                     LQARRLHWLATQSMRDELERVLQYAHLQARMQYYAVSAPDLLAAFDAGSTLVPAPVHA
                     PYTCKDLDDQKFIDLAVGHGAILVSKDKAVLCMRKRLLTLNVQVTKAIVFEGASSCIG
                     "
     gene            complement(3814..5094)
                     /locus_tag="PK518_05910"
     CDS             complement(3814..5094)
                     /locus_tag="PK518_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRM48483.1"
                     /translation="MNTLQLFLPCAAGVEGFLADEVHALTGLTGQDLLVGRGGVLVRS
                     SWRDALLLNLHSRLAQRVLVQLGERPYRGEDDIYALAADVAWEIWFTPRQSFKVEVTA
                     QHSPLKSLNFAALRVKDAVADRFRAKAGSRPNVETQWPDVRIHLHLTRELATVYIDTS
                     GEPLFKRGWRVDKGDAPLKETLAAAMIAATGWDPLGEQPLPLYDPCCGSGTVLIEAAQ
                     MACRIAPGSQRRFAFEKLLPYQQHVWIAIKNEAVSVVTKSVTPIFGSDVSHRMVDFAQ
                     RNAERAGVAASVQLRGGDALQRLPPCEQPGVMLLNPPYGERIAAAGSAGRNARERMGQ
                     VQRAGRETAQTDDGVDFFAQLAAHWKRHYAGWQAWMLTPDLKLPGKMRLKESRRVPMW
                     NGPIECRLLRFDLVAGSARARAQPPADAGAPDAS"
     gene            5207..7030
                     /locus_tag="PK518_05915"
     CDS             5207..7030
                     /locus_tag="PK518_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM48484.1"
                     /translation="MFQTLRARLIGMSIAVATLSLLGLSLVVYVVVRANMLQALDERV
                     GGLTRQYAAELTQWIGDKQQLTSSIEVAVREDNPLPFLQAAEKAGLDLAYFVMADKRH
                     AFTKPRPAGYDGTARGWYKQAVAAGGPAITPVYPDSATGNLTVSLVRPVLEGGRAVAV
                     VGSDIELSTVVKKVTGIRATEKSFAFLLDSATGNILAHANSALTLKPVADLAAGLDNA
                     LFTRLAGQRGHEVVSIDGRPQLVYAARVDGTPWMLAVVADEADALSALTRLGQTAMLS
                     TIVFVLLASVVMAAAVGRMLRRLTLVRDALQGIASGEGDLTRRLDAQGRDELAQIAQG
                     FNQFADKIAAVLLRIRQSAESVRLATSEIASGNQDLSSRTEGQASSLEETAAAMEELT
                     ATVQQNAANARQANQLAQEASQVTAQGGAAVQQVVQTMDGIHHASRKIEDIISVIDGI
                     AFQTNILALNAAVEAARAGEQGRGFAVVATEVRQLAQRSATAAKEIKALIDDSVSQVN
                     AGSRLVQDAGQTMEKVEHSIRRVSAIVAEISNASQEQSTGIAEIGDAVSQMDQATQQN
                     AALVEQASAAAQSLQQQAHELAEVVAAFRLPQGTAAQKLLA"
     gene            7165..7827
                     /locus_tag="PK518_05920"
     CDS             7165..7827
                     /locus_tag="PK518_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRM48485.1"
                     /translation="MQMTTVVTALAGALLAGAVGNAQADVVDFQDVPSGSSPCYVDFT
                     ATSLQSRGYTFTGNPADPYLYVCESGVVQNNPSAALVNANLRSVVTMTNSSGAAFSLE
                     SFFAGGRTEDFYPSLPVSLYSVATSIDILGHLMGGGTVFTSITLDSQAPYDWTQFFLP
                     SSFTNLSSVVFTAQGNGETPEFVIDDIVVTLPHQSIPEPASLALLGAALAGIGLLGRK
                     KT"
     gene            complement(7834..8271)
                     /locus_tag="PK518_05925"
     CDS             complement(7834..8271)
                     /locus_tag="PK518_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRM48486.1"
                     /translation="MARILFDLPERFGFTTELQVYISHVNYGGHLDNAQLLSLVSEAR
                     MRFFQSLGYTEGDVAGLAIVVGDMLAQYKSEAFHGETLRVQMTPRDFNRYGCDLVFCM
                     TEVSSGREVARGKTGVVLIDRVARKPAPIPDVVRKALEAVATR"
     gene            complement(8291..9835)
                     /locus_tag="PK518_05930"
     CDS             complement(8291..9835)
                     /locus_tag="PK518_05930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRM48487.1"
                     /translation="MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDDN
                     PFLELASDDGTHEPEIHEAQQPIDNATYSEAASADAEGAGAENDAETPAVAEPSDWDG
                     DGSMDAAVEDGEWGSDGIAPASGAHDGDDTEASELARSHESLTDFLHRQALSLRLSDV
                     DMAALRFLIESLNDDGYLDEPLESLAASLAGADDPEQLEELVHRFTVALRLLQNLEPT
                     GVGARDLAECLTLQLHAMNRDEEGDPAVIQTALAICSQPLDLLARRDLRRLAQATGSS
                     DDDTRAAMALIARLEPRPGRRFADVERNVIVPDVIVKKTGRGAQQDFSVQLNPDVMPR
                     LRVHEIYASALRGARGGDGHQALQSRLQEARWFIKNIQQRFDTILRVSRAIVERQKSF
                     FVHGELAMRPLVLREIADELGLHESTISRVTSAKYMATPQGTFELKYFFGSGLGTETG
                     GNASSTAVRALIKQFVAAEDVKKPLSDNQIAEMLKEQGIECARRTVAKYREALKIAPA
                     NLRKAL"
     gene            complement(9879..10649)
                     /gene="lptB"
                     /locus_tag="PK518_05935"
     CDS             complement(9879..10649)
                     /gene="lptB"
                     /locus_tag="PK518_05935"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRM48488.1"
                     /translation="MGVGPSSPAGASRLEALHLQKSYGSRKVVKDVSLVVQKGEVVGL
                     LGPNGAGKTTSFYMIVGLVRSDGGDIRIDGQSVANMPIHRRSRLGLSYLPQEASIFRK
                     LNVEDNVRAVLELQTDAAGRPLSRAEIEEQLTSLLQELRVEHLRQSPALALSGGERRR
                     VEIARALATQPRFILLDEPFAGIDPIAVIEIQRIIGFLKERGIGVLITDHNVRETLGI
                     CDHAFIISDGHVLAQGTPSDIVDNAEVRRVYLGEHFRM"
     gene            complement(10676..11317)
                     /gene="lptA"
                     /locus_tag="PK518_05940"
     CDS             complement(10676..11317)
                     /gene="lptA"
                     /locus_tag="PK518_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HRM48489.1"
                     /translation="MRNRLTILLSLGALALALLPAAHAEKADRNKPMNIEADALRHDE
                     LKQTSVFTGRVVMTKGTIVLRGAQLEVRQDPDGFQHGVVTAEPGKRAFFRQKRDTLTG
                     APDEFVEGEGEVIEYDGRTDIVKLMRRAELRRYREAQLTDELEGAIIVYNNLTDVFTV
                     DGQKTAPAGTPAGTPGGRVRAVLAPKEPASGAAAPVRGEPPALRTAPSLGGAK"
     gene            complement(11434..12066)
                     /locus_tag="PK518_05945"
     CDS             complement(11434..12066)
                     /locus_tag="PK518_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HRM48490.1"
                     /translation="MKRREFSLSAAAVAAGAVTLPAWAQPREGKDYRKLAKPAPVEAP
                     AGKVEVVEFFGYFCPHCNTFEPTLNAWAKTAPKDIVLHRVPVNFNAKTVPMQKLYYTL
                     EGMGKLEPLHAQAFRYIHVERKPLQEDEQVFAWAKGQGLDLPKFKEMYNSFGVANQIR
                     KATQLQQAYDVEGVPSMGVAGRYYVDGTMAGSMENVLRIVEQLAAQVRKG"
     gene            complement(12126..12755)
                     /locus_tag="PK518_05950"
     CDS             complement(12126..12755)
                     /locus_tag="PK518_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRM48491.1"
                     /translation="MKKQQRGGTVVGFILGVIVGLGGALAVAVYVTKVPVPFLNKNGT
                     RAPDQDAAEAKRNKDWDPNAPLYGKNPARPVTLAPEAAPAATPEAPTAAAPAPRAAPG
                     NPEALPPSADPLGDLARAKSKAAPAAGVDPFEYFVQAGAFRTQQDADAQRAKLAMLGW
                     EARVSERETNGRVVFRVRIGPFSKRDDAESLKDKLDGAGVESALVRVQR"
     gene            complement(12768..14447)
                     /gene="argS"
                     /locus_tag="PK518_05955"
     CDS             complement(12768..14447)
                     /gene="argS"
                     /locus_tag="PK518_05955"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRM48492.1"
                     /translation="MLSVKQELLAALAGELEKLSPGAGVRAAFENPKLAAHGDLACTA
                     AMQLAKPLKANPRALGEQLQTALLATPAFQQWVDAIEIAGPGFLNIRLKPAARQQVVH
                     EVLTQGARFGYQAQRGEKILVEFVSANPTGPLHVGHGRQAALGDAICNLYATQGWAVH
                     REFYYNDAGVQIDTLTRSTQLRAQGLKPGDAGWPEAAYNGDYIQDIAQAFLARSTVQA
                     DDRSFTANGDVQDYDNVRQFAVAYLRNEQDKDLQAFNLKFDEYYLESSLYSNGHVEAT
                     VARLVANGHTYEQDGALWLKSTDYGDDKDRVMKKSDGGYTYFVPDVAYHIQKWQRGFH
                     KVVNIQGTDHHGTIARVRAGLQAADVGIPQGYPDYVLHTMVRVVRGGEEVKISKRAGS
                     YVTLRDLIEWTSKDAVRFFLLSRKPDTEYTFDVDLAVAQNNDNPVYYVQYAHARICSV
                     LRGWREDFDVAALQGVDLSPLQGEQAQALMLLLAKYPEMLSAAASGNAPHDVSFYLRE
                     LAAAYHSYYDAERILVDDEAVKLARLALVAATAQVLHNGLAVLGVSAPARM"
     gene            14570..14902
                     /locus_tag="PK518_05960"
     CDS             14570..14902
                     /locus_tag="PK518_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HRM48493.1"
                     /translation="MKKLLSLLAAAGLALSFAAQAADAPAAGAAAPAKAPTAQQSKMK
                     TCNADAGAKNLKGDERKAFMKDCLSADKQAKQQDKMKSCNADAKTKNLKGDERKAFMK
                     ECLSNKPA"
     gene            complement(14960..15415)
                     /locus_tag="PK518_05965"
     CDS             complement(14960..15415)
                     /locus_tag="PK518_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HRM48494.1"
                     /translation="MITEALLAYAHIMAFLMLATFMTSETALCRQDWINPAVVRRLVW
                     VNLLTLVSGALVLATGLARIAWGVKGSAWYWGQPLLHAKITLLVMMLGLALLARRRYL
                     RWRQTLDTQGSLPAESDLRGTRRLVMWAAHLMVLVPLLAVFLARGVGTR"
     gene            complement(15437..>16351)
                     /locus_tag="PK518_05970"
     CDS             complement(15437..>16351)
                     /locus_tag="PK518_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM48495.1"
                     /translation="FVSVATKGSLTAAARAEGVAPAIMGRRLDALEEHLGVKLLVRTT
                     RRISLTHEGSAFLEDCQRVLADVANAEASVSAGGVRATGHLRITAPAGFGRRHVAPLV
                     PRFHDLHPEVTISLNLSDRVVDLAGEGYDCAVRVGDLPDSSLVSVRMADNRRLCVATP
                     RYLQRHGTPMHPGELAQHDCLTLSSDASQTRGWAFRVPVEGGGGATEVVHLKPGGPLD
                     CSDGQVLHDWCLGGWGIAWRSTWEVEAEIAAGRLVAVLEDFAAPPNGIYVVFPQRKHL
                     PLRVRLWIEYLKHHYAQTEFWGRPAGSL"
     assembly_gap    16352..16451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16764..18959
                     /locus_tag="PK518_05975"
     CDS             16764..18959
                     /locus_tag="PK518_05975"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HRM48496.1"
                     /translation="MTARTQVHGLQVANELHSFINQQVLPGTGVDPAAFWQGFDAIVA
                     DLAPKNAALLAERDRLQKELDTWHKANPGPISDMAAYRAFLSRIGYLVEPPQGAKAST
                     ANVDAELAIQAGPQLVVPILNARYALNAANARWGSLYDALYGTDVIDEEGGAEKGKGY
                     NPVRGAKVIEFARNFLDQAAPLGAGSHRDAIAYTVADGKLAVTLKGGTATGLKDAAQF
                     VGYQGDAAAPASVLLVNNGLHIDIQIDKNTAIGKSDAAGVADIVLESALSTILDLEDS
                     VAAVDADDKVLGYSNWLGILKGTLTETFDKGGKQLTRGLNPDREYLAGDGKSVKLHGR
                     SLLFVRNVGHLMTNPAILWGKEGREIPEGIMDAVVTTAIALHDLKGLATAGIKNSRTG
                     SVYIVKPKMHGPAEVGFANELFGRVEQLLGLAENTVKLGIMDEERRTSVNLKACIAAA
                     PARVAFINTGFLDRTGDEMHTAMQAGPMIRKGDMKTSAWIQAYEKSNVLVGLGMGLRG
                     KAQIGKGMWAMPDLMKAMLEQKIAHPKAGANTAWVPSPTGATLHALHYHQVKVSDIQI
                     QLEKTDADAERDNLLTGLLTVPVSSNPNWSTEEKQQELDNNIQGILGYVVRWVDQGVG
                     CSKVPDINDIGLMEDRATLRISSQHVANWLLHGIVSEGQVRATLERMAAKVDQQNSGD
                     PLYQRMHGRFDQSMAYQAACELVFKGVEQPSGYTEPLLHAWRLKVKAAA"
     gene            19063..19275
                     /locus_tag="PK518_05980"
     CDS             19063..19275
                     /locus_tag="PK518_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HRM48497.1"
                     /translation="MPESNSDCPLCGQSNQCAMVAGLPATRCWCMTQPVAPAALQQLP
                     PERRGLACICPECARPLPPMQQSLPI"
     gene            19303..19491
                     /locus_tag="PK518_05985"
     CDS             19303..19491
                     /locus_tag="PK518_05985"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HRM48498.1"
                     /translation="MPTYHIEMLEGRTVEQKRLLAEEITRVSVEILGGSPDSVDILIT
                     DVKRENWATGGKLWLERQ"
     gene            19505..20272
                     /locus_tag="PK518_05990"
     CDS             19505..20272
                     /locus_tag="PK518_05990"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRM48499.1"
                     /translation="MCQLLGMNANTPTDVTFSFTGFAQRAGGTADHTDGWGIAFFEGR
                     GLRHFVDHERAIDSPVAELIRRYPIKSRNVIAHIRKATQGVVSLENCHPFVRELWGRY
                     WVFAHNGDLKDFRPRLHAHFHPVGSTDSEHVFCWLMQELSKSHAGMPRIEELTLTLRE
                     LAARIAPHGTFNFLLSNGQALWAHASTSLYYIERRHPFSQAQLCDEDMRVDFSALTTP
                     KDKVAVVVTAPLTTNEEWTAFQSGEMRVFVDGECLAE"
BASE COUNT         3536 a         6760 c         6489 g         3480 t
ORIGIN      
        1 gccgccacgc tggcgcgcct gcgcgccagg ctggagcgcc ccacgccgcg cgtggcgctg
       61 gggctggcgc tgcgcggcct ggcgagcagc gccatcgatg tcagcgacgg cctgctgggc
      121 gacctgggcc atgtcctcaa gtcctcccgc gtcggggccg agatcgacgt ccacatcact
      181 actgatttgc tagcttctgg cgcgctacag gaaaatgcta aaggcatttt tgatgcagaa
      241 accatgatgc catgtgcact cgccggtggc gacgactacg agctggtctt caccgcccct
      301 gccgcacggc gcgccgccgt gcaggccgca tccagtgcca gcggtacgca ggtcacacgc
      361 ataggccgca tcacgcccgc gcccggcctg cgcctgctcg atcgcacggg ccagccattg
      421 ccggggcgct tcgcctcgtt cgaccacttt gcctgaatga cccaccctca ccccccattg
      481 cccacaaccc tgctggagcg cgcacgcacg ccgggcagcc cgtccgtgga tctgctcatg
      541 gtgtgcatgg gcaacatctg ccgcagcccc acggccgagg gcgtgctgcg cgccctggtg
      601 cagcgcgccg gcctgcaaag gcgcatcacg gtggattcgg ccggcaccca tggctttcat
      661 gtcggcgtgg cgcccgacaa gcgctcccag cagcatgcgg cgcggcgcgg ctacgacctc
      721 tcgcgcctgc gcgcgcgcca gctcacggcg cgcgacttcg agcgcttcga cctggtgctg
      781 gtcatggacg aggccaatga gcaggccgcc ctggcgctgt gcccgcccgc ccatgccggg
      841 cgcgtgcacc gcctcacgca gttctgcctg cgccaccaag ccaacgccgt gcccgatccc
      901 tactatggcg gcgcccaggg ctttgagcag gtgctggatc tggtggagga cgcctgcgcc
      961 ggcctgctcg ccgcgctgca ggcgcgtcag ccctgatggc atgatgcgcc cctcatgaat
     1021 cctgccgccg ccccccgccg ccccacgccg gcctttctgc tcgcccaccc ggcccactgc
     1081 atcgcgctgg gctttggcag cggcctgtcg cccaaggcac cgggcacggt gggcacggcc
     1141 tggggctggc tggcgtttct ggtgcttgag ctgtggctca cacccctcgg catgggcctg
     1201 gtgatcggct tctcgctgtt gctcggctgg tgggcctgca ccgtcaccgc gcgccacatg
     1261 ggcgtggccg atccgggcag catcgtctgg gacgagatcg tcgccatctg gatcgtgctc
     1321 tggctggcca tgcccatggg cttctggggc cagctcgcgg cattcgcgct gtttcgctac
     1381 ttcgacgccg ccaagcccgg ccccgtggcc tgggcagatc ggctgttcaa gggctttggc
     1441 tggcgcggcg gctggggcat tctgtttgac gacctggtgg ccgcgggctg cacgctgctg
     1501 gtgctggcgc tgtggcgcca ccttttcggg tgagccccat gacactatca aaacatgagc
     1561 tgtcagcact tgatgacaaa gcgctacagg ccgatttgac tcatatttcc agcagcctgc
     1621 tggcgcatgg ctggatgctg gccacggccg agagctgcac cggcggcctg atcgccgccg
     1681 cctgcaccga cctggcgggc tccagccaat ggttcgagcg cggctttgtg acctactcca
     1741 acgccgccaa ggtgcaatgc ctgggcgtgc ccgccgacct catcgagcgc catggcgcgg
     1801 tcagcgaggc cgtggcccgc gccatggccc agggcgccgt ggcgcacagc gcggcccagg
     1861 tggccctggc cgtgaccggc gtggccggcc ctacgggcgg ctcggccgac aaacccgtgg
     1921 gcacggtgtg gtttggctgg caggtacagg gcgccacgca cagcgagctg cgccgctttg
     1981 ccggtgaccg cgccgccgtg cgcgcgcaga cggtgcgcca tgcgctggcg cggctggcgc
     2041 ggctgctggg ctgagagcct gagccaggct cacgccccgc agcacttctt gtgcttcttg
     2101 ccgctgccgc aggggcaggg gtcgttgcga ccgggttggg ggccgtggac tatggcctgc
     2161 acacgcgggc ccaggctgcg ccagagctgg cgcaggtcgt acaccgccca gatggcctcg
     2221 ccgaaatcct ccaggcgctg ctggctggtg ctgggcgggc cgtcctcgtc atacatgcag
     2281 atcgtcggct tgccggtgtc gtcctcggcc agggcgacga tggcctgcag cgcatcgtcc
     2341 agccactggg cagcctcctt gtcgcgcggc gcctgccatt cgtcgtccca gttctccacg
     2401 gcgaacatga agcccagggc ccagatctgg ccgaaggacg gtatcggctg accctccatc
     2461 tcggcgcgct cgtcctcggg caggctggcg atggcgccac gcatgtccag cacctcgggc
     2521 tggaaggcgt catcggcatc cagcgactcg gcctgggcgt ccagctgggc cgccacttcg
     2581 gcgctgcgca actggcacca atgcacaaag cgctgctgct gcgccagatc cttgaacgga
     2641 ggcatgagcg gcagtgcacc cgccgcatcc agccccagct ccaggccatc gcccaccagc
     2701 atgggcagcc attcggcgac cgaaatcgca cggcgcgtgc agaccagcgc agtcaggaag
     2761 ccgtcgcaga actcccactg cggcacctcg tcctcgcgcg tgcgcaggtc ttcgaggatc
     2821 aggtcgagtt cgtcgagcgc gtcgggcccg agggccggat cgggggcggc ccggttgttg
     2881 tcgatggtgg tcatggcggt attccggcgc aaggcagcga gggggatagg atgcgccagt
     2941 gtagcgcctc gcctgccgtg gaactattgt tttcatagct tgttcgcatt gcaatgcgcg
     3001 gcctacgggt cataaagcag cacaaaacaa aacgccccat gccggtcagg cagtggggcg
     3061 tgaacatggt gatgcgggcc cgcaggccgc gcgcatcact gataggcgac gggcaggcgt
     3121 gcctggcggg cttcttggat gaactcgatg atcgagagga tggttgcgcg catgatgatt
     3181 cctgtttgag atgcccatcc agtgcatggc ggcacgcatc tcaagcgcct cccaacaccg
     3241 ggttaacccc taagtgttaa ccctatggca ttagtataaa ccacatccct tgttttatgg
     3301 atggcgcggc atacccacct gcttgtgcag gcgttcacag atcagccgat acaggacgat
     3361 gcgccctcaa aaacaatagc tttcgtcacc tgcacattca gggtcagcag ccgttttcgc
     3421 atacagagaa cggccttgtc cttggacacc agaatcgcgc catgtccgac cgccaggtcg
     3481 atgaacttct ggtcatccaa gtccttgcat gtgtaagggg catgaaccgg cgccggcacc
     3541 agcgtgctgc cggcgtcgaa ggccgccagc agatccggtg cagacacggc gtagtactgc
     3601 atgcgtgctt gcaggtgtgc gtattgcagc acgcgctcca gctcatcgcg catggactgg
     3661 gtggccagcc agtgcaggcg ccgcgcctgc agcaggccgc gcagcggggc cacggccggg
     3721 tcggcaaaga tgaacagatc cagcacgatg ttggtgtcca gcaccacggc tcgcgcgccc
     3781 tcgccggcct gggtccgtgc cggcaggcgc agctcaggat gcatcgggcg cccccgcatc
     3841 ggccggcggc tgggcacgcg cgcgcgccga tccagccacc aggtcaaagc gcagcaaccg
     3901 gcattcgatg gggccgttcc acatgggcac gcggcgcgat tccttcaggc gcatcttgcc
     3961 cggcagcttg aggtcgggcg tgagcatcca ggcctgccag ccggcgtagt ggcgcttcca
     4021 gtgcgcagcc agctgggcga agaagtccac accgtcgtct gtctgcgccg tctcgcgccc
     4081 ggcgcgctgc acctggccca tgcgctcgcg tgcgttgcgg cccgcgctgc cggcggcggc
     4141 gatgcgctcg ccatagggcg gattcagcag catcacaccc ggctgctcac agggcggcag
     4201 gcgctgcagc gcgtcgccac cgcgcaactg cacgcttgct gccacgccgg cacgctcggc
     4261 attgcgttgg gcaaaatcca ccatgcggtg cgacacatca ctgccaaaaa tgggcgtaac
     4321 gctcttagtt actacgctga cagcttcatt ttttatagcg atccagacat gttgctggta
     4381 gggcagcagc ttctcgaagg caaagcggcg ctggctgccg ggtgcgatgc ggcaggccat
     4441 ctgcgctgcc tctatcagca ccgtgccgct gccgcagcag gggtcataca gcggcagggg
     4501 ctgctcgccc agcggatccc agccggtggc ggcgatcatg gcggcggcca gggtttcctt
     4561 caacggcgcg tcacccttgt ccacgcgcca gccgcgcttg aagagtggct cgcccgaggt
     4621 gtcgatatac accgtggcca gctcgcgcgt caggtgcagg tggatgcgca cgtcgggcca
     4681 ctgcgtctcg acattcgggc ggctgccggc cttggcgcga aagcggtcgg cgacggcgtc
     4741 cttcacgcgc agcgcggcaa agttcaggct cttgagcggg ctgtgctggg ccgtgacctc
     4801 gaccttgaag ctctggcgcg gggtgaacca gatctcccag gccacatccg ctgccagggc
     4861 gtagatgtcg tcctcgccgc ggtagggccg ctcgcccagt tgcaccagta cgcgttgggc
     4921 caggcggctg tgcaggttga gaagcagcgc atcgcgccag ctggagcgca ccagcacgcc
     4981 gccacggccg acgagcagat cctggcccgt cagcccggtg agcgcgtgca cctcatcggc
     5041 cagaaagccc tcgacgccgg cggcgcaggg caggaatagt tgcagggtgt tcataaagcc
     5101 cgccaagcat acgcgctgcg cggtagtggt ttacccgagg ctgctgaaat cacaaaatac
     5161 aatcattaac atttaaaacg aattcaacac atacgctgga gaccccatgt ttcagaccct
     5221 gcgcgctcgc ctgatcggca tgagcattgc cgtcgccacc ctgtccctgc tggggctgtc
     5281 gctggtggtg tacgtggtgg tacgcgccaa catgctgcag gcgctggacg agcgcgtggg
     5341 cggcctgacg cggcagtacg ccgccgagct gacgcagtgg attggcgaca agcaacagct
     5401 caccagctcc atcgaggtgg cagtgcgcga ggacaacccc ctgccctttt tgcaggccgc
     5461 cgaaaaggcc gggctggatc tggcctactt cgtgatggcc gacaagcgcc atgccttcac
     5521 caagccgcgc cccgctggct atgacggcac ggcacgcggc tggtacaagc aggccgtggc
     5581 cgccggcggc cccgccatca cacccgtata ccccgattcg gcgaccggca acctcacggt
     5641 cagcctggtg cggccggtac tcgaaggcgg ccgggcggtc gccgtggtgg gctcggacat
     5701 cgagctgtcc acggtggtga aaaaggtcac cggcatacgc gccacggaga agagctttgc
     5761 cttcctgctc gacagtgcca cgggcaacat cctggcgcac gccaacagcg cactgacgct
     5821 caagccggtg gccgacctgg cggcggggct ggacaacgcc ttgttcacgc gcctggccgg
     5881 ccagcgcggg catgaggtgg tgagcatcga tggccggccg caactggtct acgccgccag
     5941 ggtcgatggc acgccctgga tgctggccgt ggtggccgac gaggccgacg ccctgtccgc
     6001 cctgacccgg ctgggccaga ccgccatgct cagcaccatc gtctttgtgc tgctggccag
     6061 cgtggtgatg gcggccgcgg tggggcgcat gctgcgccgc ctgacactgg tgcgcgacgc
     6121 gctgcagggc attgcctcgg gcgagggcga cctgacaaga cgcctggatg cgcagggccg
     6181 cgatgagctg gcgcagatcg cccagggctt caaccagttt gccgacaaga tcgccgccgt
     6241 gctgctgcgc atccgccagt ccgccgaatc ggtgcgcctg gccaccagcg agatcgccag
     6301 cggcaaccag gatctgtcct cgcgcaccga gggccaggcc agttcgctgg aggaaaccgc
     6361 cgccgccatg gaagagctca cggccacggt gcagcagaat gccgccaacg ccaggcaggc
     6421 caatcagctg gcgcaggagg cctcgcaggt cacggcgcag ggcggcgcgg cggtgcagca
     6481 ggtggtgcag accatggacg gcatacacca cgcctcgcgc aagatcgagg acatcatcag
     6541 tgtcatcgac ggcattgcct tccagaccaa catcctggca ctgaacgccg cggtcgaggc
     6601 cgcccgcgcc ggcgaacagg gccgcggctt tgccgtggtg gccaccgagg tgcgccagct
     6661 ggcgcaacgc agcgccacgg cggccaagga gatcaaggcc ctgatcgacg actcggtgag
     6721 ccaggtgaac gccggcagtc gcctggtgca ggacgcgggc cagaccatgg aaaaggtgga
     6781 gcacagcatt cgccgcgtca gcgccatcgt ggccgagatc agcaacgcca gccaggaaca
     6841 aagcacgggc attgccgaga tcggcgacgc cgtctcacaa atggatcagg ccacgcagca
     6901 gaacgccgcc ctggtggagc aggccagcgc cgccgcccag tcgctgcagc agcaggccca
     6961 tgaactggcc gaagtggtgg cggccttcag gctcccgcag gggacggccg cgcaaaagtt
     7021 gctggcctga cgctgaaatt tgtcagattt ctttacatca ctcgcggggc aatcgagacc
     7081 aagtcataaa aatcaacagc ttggggtgct ggcacatgat atgcatgggt gtttcacgtc
     7141 ttacaactga cgggagatgc caccatgcaa atgaccactg ttgtgaccgc ccttgcgggg
     7201 gcattgttgg cgggtgccgt tggcaacgcc caggcggatg tggttgattt tcaggacgtg
     7261 ccttcgggca gctccccctg ttatgtcgat ttcaccgcca cctcactgca aagccggggc
     7321 tataccttca ccggcaaccc cgcagacccc tatttgtatg tctgcgaatc aggggttgtg
     7381 caaaacaatc catcggcagc cctggtcaac gcgaatctcc gatccgtcgt gaccatgacc
     7441 aatagcagcg gggctgcgtt ttccctggag tcattttttg cgggtggcag aacggaagat
     7501 ttttacccgt cgctgcccgt gagcttgtac agcgtcgcca ccagcatcga catccttggg
     7561 cacctcatgg gcggcggcac ggtgtttacc agtatcacgt tggacagcca ggctccctat
     7621 gactggacgc agttcttcct gccgtcatcg ttcaccaacc tgagttcggt ggtgttcact
     7681 gcacaaggca acggtgagac gccggaattc gtgatcgacg atatcgtggt cacgttgccc
     7741 caccagtcca ttcccgagcc ggcatccctc gccttgctcg gcgccgcgct ggctggtatt
     7801 ggcctgttgg ggcgaaaaaa aacctgacgc gcgtcagcgt gtcgccacgg cctccagggc
     7861 cttgcgcacg acatccggaa tgggcgccgg cttgcgtgcc acgcggtcga tgaggaccac
     7921 gccggtcttg ccgcgagcca cctctcggcc cgaggagacc tctgtcatgc agaacaccag
     7981 gtcacagccg tagcggttga agtccctcgg cgtcatctgc acgcgcagcg tctcgccgtg
     8041 aaaggcctcg gacttgtatt gcgccagcat gtcgccgacc acgatggcca ggcccgccac
     8101 atcaccctcg gtgtagccga gcgactggaa gaagcgcatg cgcgcctcgg acaccaggct
     8161 cagcagctgg gcgttgtcca gatggccgcc gtagttgacg tggctgatat agacctgcaa
     8221 ctcggtggtg aagccgaagc gttcgggcag gtcgaagagg atgcgggcca tgggaatcta
     8281 tgccttgtgt ctatagcgcc ttgcgcagat tggccggcgc gatcttcagc gcctcgcggt
     8341 acttggccac ggtgcgacgc gcgcactcta tgccctgctc cttgagcatc tccgcgatct
     8401 ggttgtccga caacggtttc ttcacatcct cggcggcgac gaactgcttg atcagcgcgc
     8461 gcaccgcggt gctcgacgcg ttgccgccgg tctcggtgcc caggccggag ccaaagaagt
     8521 acttcaactc gaacgtaccc tgcggcgtgg ccatgtactt ggcactggtc acgcggctga
     8581 tggtggactc gtgcaggccc agctcgtcgg cgatctcgcg cagcaccagc ggccgcatgg
     8641 ccagctcgcc atgcacgaag aagctctttt gccgctcgac gatggcgcgc gatacacgca
     8701 agatagtgtc aaatcgctgc tgtatgttct tgatgaacca gcgtgcctcc tgcagccggg
     8761 actgcagcgc ctggtgcccg tcgccgccgc gcgcaccacg cagggcgctg gcatagatct
     8821 cgtgcacgcg caggcgcggc atgacgtcgg ggttgagctg cacgctgaag tcctgctgcg
     8881 cgccgcgccc ggttttcttc acgatgacgt cgggcacgat gacgttgcgc tccacgtcgg
     8941 caaagcgccg gccggggcgc ggctccagac gggcaatcag cgccatggcg gcgcgcgtgt
     9001 cgtcgtcgct gctgcctgtg gcctgggcca ggcggcgcag gtcgcgccgc gccagcagat
     9061 ccagcggctg gctgcagatg gcgagcgcgg tctgaatcac cgccgggtcg ccctcttcgt
     9121 cgcggttcat ggcgtgcagc tgtagcgtca ggcattcggc caggtcgcgc gcgcccacgc
     9181 cggtcggctc caggttttgc agcaggcgca gggccacagt gaagcggtgc accagctctt
     9241 cgagctgctc ggggtcgtcg gcccccgcca ggctggccgc cagggattcc agcggctcgt
     9301 ccaggtagcc atcgtcgttc agcgactcga tgagaaagcg cagcgccgcc atgtcgacat
     9361 cggacaggcg cagtgacaat gcctggcgat gcaggaaatc ggtcagggat tcgtggctgc
     9421 gcgccagctc gctggcctcg gtgtcgtcac cgtcatgcgc gccgctggcg ggcgcgattc
     9481 cgtcgctgcc ccattcgccg tcttccaccg cagcatccat gctgccgtca ccgtcccagt
     9541 cactgggctc ggcaaccgct ggggtttcgg catcattttc ggctccagcg ccctctgcat
     9601 cagcgctagc agcttctgaa tatgtagcat tgtcaatcgg ctgctgcgct tcatggatct
     9661 caggctcatg cgtcccatcg tcggacgcca gctccagaaa cgggttgtca tcgagcatct
     9721 gctcgacttc ctgcgccagc tccagggtgg acagctgcag caggcgaatc gattgctgca
     9781 actgcggcgt aagcgccaga tgctgcgaaa cgcgcagtga caggccgggc ttcatggccg
     9841 ccctccttgc atctggatgc tgcgcctgct caccacgctc acatgcggaa atgctcgccc
     9901 aggtacaccc ggcgcacttc cgcgttgtcc acaatgtccg acggcgtgcc ctgggcgagt
     9961 acatggccgt cgctgatgat gaaggcgtgg tcgcagatgc ccagcgtctc gcgcacgttg
    10021 tggtcggtga tcaagacgcc aatgccgcgc tctttcagga agccgatgat gcgctggatc
    10081 tcgatcacgg caatgggatc gatgccggca aagggctcgt ccagcaggat gaagcgcggc
    10141 tgcgtggcca gcgcccgggc aatctccacg cgccggcgct cgcccccgga cagggccagc
    10201 gccggcgact ggcgcaggtg ctcaacgcgc agctcctgca gcaggctggt gagctgctcc
    10261 tcgatctcgg cgcgcgagag cgggcgcccg gcggcgtcgg tctgcagctc cagcaccgcg
    10321 cgcacgttgt cttccacgtt gagcttgcga aagatcgagg cctcttgcgg caggtaggac
    10381 aggcccaggc gcgaacgccg gtggatcggc atgttggcca ccgactggcc gtcgatgcgg
    10441 atgtcgccgc catcgctgcg caccaggccc acgatcatgt agaacgaggt ggtcttgccc
    10501 gcgccattgg ggcccagcag gcccaccacc tcgccctttt gcactaccag ggacacatcc
    10561 ttgaccacct tgcggctacc gtaggacttt tgcaggtgca aggcctccag ccggctcgca
    10621 cccgcagggg agctgggccc cacgcccacg gcgcgctcgt cgctcacggc ctggctcact
    10681 tggcgccgcc caggctgggg gcggtgcgca gcgcgggcgg ttccccgcgc accggcgccg
    10741 ccgcgcccga tgccggctcc ttgggcgcca gcacggcacg cacgcgcccg cccggcgtgc
    10801 ccgccggggt gccggccgga gccgtctttt gcccatccac cgtgaacacg tcggtaaggt
    10861 tgttgtagac gatgatcgcg ccctccagct catcggtgag ctgggcctcg cggtagcggc
    10921 gcagctcggc acggcgcatc agcttgacga tgtcggtacg gccgtcgtac tcgatgacct
    10981 cgccctcgcc ctcgacgaac tcatccggcg cccccgtcag cgtgtcgcgc ttctggcgga
    11041 agaaggcgcg cttgcctggc tcggccgtga ccacgccatg ctggaaaccg tctggatcct
    11101 ggcgcacctc cagctgcgcg ccacgcagca cgatggtgcc cttggtcatc accacacggc
    11161 cggtgaacac gctggtctgc ttgagctcat cgtggcgcag cgcatcggcc tcgatgttca
    11221 tcggcttgtt gcggtcggcc ttttcggcat gggccgcggg cagcaaggcc agcgccaggg
    11281 cgccaagcga aagaaggatg gtcagtcggt ttctcataag gcacgattct tgcggtcatt
    11341 gtactgatca ccgtggccat cctggagagc ggccgcgcag gggcgaaaaa aagcgcgccc
    11401 agggcgcgct cttgtgtgtt gccacggctc gcttcagccc ttgcgcacct gcgcggccag
    11461 ttgctccacg atgcgcagca cgttctccat gctgccggcc atggtgccgt ccacatagta
    11521 gcgcccggcc acgcccatcg agggtacacc ctccacgtca taggcctgct gcagctgcgt
    11581 ggccttgcgt atctggttgg cgacgccgaa ggagttgtac atctccttga acttcggcaa
    11641 gtccaggccc tgccccttgg cccaggcaaa cacctgctcg tcctcctgca ggggcttgcg
    11701 ctccacatgg atgtagcgga aagcctgggc gtgcagcggc tcgagcttgc ccatgccctc
    11761 gagcgtgtag tacagcttct gcatgggcac ggtcttggcg ttgaagttga ccggcacacg
    11821 gtgcagcacg atgtccttgg gcgccgtctt ggcccaggcg ttgagcgtgg gctcgaaggt
    11881 attgcaatgc gggcagaaat agccaaagaa ctcgaccacc tcgaccttgc cggcgggcgc
    11941 ctcgacggga gcgggcttgg ccagtttgcg gtagtccttg ccctcgcgcg gctgggccca
    12001 ggcgggcagg gtcacggcgc cagcggccac tgcagcggcg gaaagagaaa actcacggcg
    12061 tttcatgggg tttgaacact cctatgtcgg tacgccgact tggaagccgg cgcggggata
    12121 aaagttcaac gctgcacgcg caccagggcc gactcgacgc cagcaccgtc cagcttgtcc
    12181 ttcagcgact cggcatcgtc acgcttggag aacgggccta tgcgcacccg gaacaccacg
    12241 cggccattgg tttcacgctc gctcacgcgc gcctcccaac ccagcatggc cagcttggca
    12301 cgctgcgcgt cggcatcctg ctgggtgcga aaggcaccgg cctgcacaaa atattcaaac
    12361 ggatccacgc cggccgcggg cgcggccttg gacttggcgc gggccagatc gcccagcggg
    12421 tcggccgacg gcggcaatgc ctcagggttg ccgggcgcgg cgcgcggcgc aggcgccgcg
    12481 gccgtgggcg cctccggtgt ggctgcgggc gccgcttccg gcgccagcgt caccggtcgc
    12541 gccgggttct tgccgtacag cggcgcgttg ggatcccagt ccttgttgcg cttggcctcg
    12601 gccgcatcct gatcgggcgc acgcgtgccg ttcttgttca ggaatggcac cggcaccttc
    12661 gtaacgtaca cggccacggc cagcgcgccc cccaacccga caatcacccc gaggatgaat
    12721 cccacgacgg tgccgccgcg ttgctgcttt ttcatatgca tcgtgaccta cattctggcc
    12781 ggcgcggaca cgcccagcac cgccagccca ttgtgcaata cctgagcggt ggcggcgacg
    12841 agtgccaggc gggccagctt cacggcctcg tcatcgacca ggatgcgctc ggcgtcgtaa
    12901 tagctgtggt aagcggctgc cagctcgcgc aggtaaaagc tcacgtcatg cggcgcgttg
    12961 ccgctggcgg cggcgctcag catctcgggg tacttggcca gcagcagcat cagcgcctgg
    13021 gcctgttcgc cctgcagggg cgaaagatcc acaccctgca atgccgccac atcaaagtct
    13081 tcacgccagc cacgcagcac cgagcagatg cgcgcatgcg cgtactgcac gtaatacacc
    13141 gggttgtcat tgttctgggc cacggccaga tcgacgtcga aggtgtattc ggtgtcgggc
    13201 ttgcggctca agaggaagaa acgcaccgcg tccttgctgg tccactcgat caggtcgcgc
    13261 agcgtgacgt aggagccggc gcgcttgctg atcttgacct cctcgccgcc acgcaccacg
    13321 cgcaccatgg tgtgcagcac atagtcgggg tagccctggg gaatgccgac atcggccgcc
    13381 tgcaggccgg cgcgcacgcg ggcgatggtg ccgtggtggt ccgtgccctg gatgttcacc
    13441 accttgtgaa agccgcgctg ccatttctgg atgtggtagg ccacgtccgg cacaaagtag
    13501 gtgtagccgc cgtcgctctt cttcatgacg cggtctttgt cgtcgccgta gtcggtgctc
    13561 ttgagccaca gcgcgccgtc ctgctcgtag gtatggccgt tggcgaccag gcgcgccacc
    13621 gtggcctcca catggccgtt gctgtacagg ctggactcga ggtagtactc gtcgaacttc
    13681 aggttgaacg cctgcagatc cttgtcctgc tcgttgcgca gataggccac ggcgaactgg
    13741 cgcacgttgt cgtagtcctg tacgtcgcca ttggccgtga agctgcggtc gtcggcctgt
    13801 accgtgctcc tggccagaaa ggcctgggca atgtcctgga tgtagtcgcc gttataggcg
    13861 gcctcgggcc agccggcatc accgggcttg agcccttgtg cgcgcaactg tgtgctcctg
    13921 gtcagggtgt cgatctgcac gccggcgtcg ttgtaataga actcgcggtg cacagcccag
    13981 ccctgggtgg cgtagaggtt gcagatcgcg tcgcccaggg ccgcctggcg gccatggccc
    14041 acatgcagcg gccccgtcgg gttggcggag acgaactcga ccaggatttt ttcaccgcgt
    14101 tgggcctgat agccgaaccg ggcaccctgc gtgagcacct catgcaccac ctgctgcctg
    14161 gcagccggct tcaagcgaat gttcaagaag ccggggccgg cgatctcgat ggcgtccacc
    14221 cactgctgaa acgcgggggt cgccagcaaa gcggtttgca attgttcacc cagcgcacgc
    14281 ggattggcct tgagcggctt ggccagctgc atggcggccg tgcaggccag gtcgccatgg
    14341 gcggcgagct tggggttttc aaaggcggcc cggacgcccg cgcctggcga gagtttttcc
    14401 agctcgccgg ccagcgccgc gagcaattcc tgtttgacag agagcatggg ggcggattct
    14461 acgggtcgcc ccgtcatcgc ggcgcaccat ccgggcgtta tgctgggtgc cccgcatggc
    14521 acagcgcgtc cggcgggccc atcactatcc accattggag gagcgttaca tgaagaagct
    14581 gctttccctg ctggccgccg ccggtcttgc cctttcgttc gccgcccagg cagccgatgc
    14641 cccagccgca ggcgccgctg cccctgccaa ggctcccacg gcccagcaaa gcaagatgaa
    14701 gacatgcaat gccgacgccg gcgccaagaa cctcaagggc gacgagcgca aggccttcat
    14761 gaaggactgc ctgtccgccg acaagcaggc caagcagcag gacaagatga agtcctgcaa
    14821 cgccgacgcc aagaccaaga acctcaaggg tgacgagcgc aaggccttca tgaaggaatg
    14881 cctgagcaac aagccggcct gaaccgcact gccgacaccc gccaacccgt gcgccgcttg
    14941 ccggcccacg ggtttttgct tatcgcgtgc caacaccccg cgccaggaac accgccaaca
    15001 gaggcaccaa caccatcagg tgcgcggccc acatgaccag gcggcgcgta ccgcgcagat
    15061 cggactcggc cggcaggctg ccctgcgtgt ccagcgtctg gcgccagcgc aggtaacgcc
    15121 gccgcgccag cagggccagg cccagcatca tcaccagcag cgtgatcttg gcgtgcaaca
    15181 gcggctgacc ccagtaccag gcactgccct tgacgcccca ggcgatgcgc gccagcccgg
    15241 tggccagcac cagcgcgccg gacaccaggg tcaacagatt cacccagacc aggcgccgca
    15301 cgacggcagg attgatccag tcctggcggc acagcgcggt ctcgctggtc ataaaggtgg
    15361 ccagcatcaa gaacgccatg atgtgggcgt aggccagcaa ggcttcggtg atcacaaact
    15421 tcctttcatc aatgcgtcaa aggctgccgg ctggccggcc ccagaattcg gtctgcgcgt
    15481 aatggtgctt gaggtattcg atccacagcc gcacccgcag aggcaggtgc ttgcgctggg
    15541 gaaagaccac gtagatgcca ttgggcgggg cggcgaaatc ctccagcacc gccaccagac
    15601 ggccggcggc gatctcggcc tcgacctccc aggtgctgcg ccaggctatg ccccagccgc
    15661 ccaggcacca gtcgtgcagc acttgtccat ccgaacagtc gagcggcccg ccgggcttca
    15721 ggtgcacaac ctcggtggcg ccgccgccac cctccaccgg cacacgaaac gcccagccac
    15781 gggtctgcga ggcatcgctg gacagggtca ggcagtcgtg ctgggccagc tcacccgggt
    15841 gcatgggcgt gccgtggcgc tgcagatacc gcggtgtggc gacgcacagg cgtcgattgt
    15901 cggccatgcg cacgctgacc agggacgaat ccggcagatc gcccacgcgc acggcgcagt
    15961 cgtaaccctc gccggccaga tccaccacac ggtcactcaa attgagggaa atcgtcacct
    16021 cgggatgcag gtcatgaaac cgtggcacca acggtgcgac atggcgccgg ccaaaaccag
    16081 ccggcgccgt gatgcgcaaa tgccccgtgg ccctgacccc acccgccgag acgctggcct
    16141 cggcattggc tacatcggcc agcacgcgct ggcagtcctc caaaaaggcg ctgccttcgt
    16201 gcgtcaggct gatgcgccgc gtggtacgca ccaggagctt gacacccaga tgctcttcca
    16261 gcgcatccag gcgccgcccc atgatggcgg gcgccacgcc ctcggccctg gcagcagctg
    16321 tcaggctgcc cttggtagcc acggagacga annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn ncgccctcgg ccctggcagc agctgtcagg ctgcccttgg tagccacgga
    16501 gacgaaggac tcgaaggctt tgagtttgtc catgaaattg gctccaacca ggcttatgca
    16561 aaggtttgac gcgcgctgaa tgctatcatt ttttagagca agtgcgctac gggaacgcgg
    16621 atttgcgccg tcgattttta caaaaaagtc attggttaag agatattcac gcggtttatt
    16681 aatgcgtata tttctaatac agtgctgtgc aaggctcggg gctagaagca ccgaacgtcg
    16741 tcaatcattg tcaggggatt ctcatgaccg cacgcaccca agtccatggc ctgcaggtag
    16801 ccaatgagct gcacagcttc atcaaccagc aggtgttgcc cggcacaggg gtcgatcccg
    16861 ccgctttttg gcagggtttc gacgccatcg tcgccgatct ggcgccaaaa aacgccgcct
    16921 tgttggccga gcgcgatcgc ctgcaaaaag agctggacac ctggcacaag gccaatccag
    16981 gccccatctc ggacatggcg gcataccgcg cttttctctc gcgcattggc tacctggtcg
    17041 agccacccca aggggccaag gccagcacgg ccaacgtgga cgccgagcta gccatccagg
    17101 ccggccccca gctggtggtg cccatcctga atgcgcgcta cgcactcaat gccgcaaatg
    17161 cgcgctgggg ttcgctgtat gacgccctgt acggcacgga cgtcatcgac gaagagggcg
    17221 gcgccgagaa gggcaagggc tacaacccgg ttcgtggtgc caaagtcatc gaatttgcgc
    17281 gcaacttcct cgatcaggct gcccctctgg gcgctggctc gcaccgtgat gccatcgctt
    17341 atacggtcgc agacggtaag ctggctgtca cgctcaaggg tggcacagcc accggcctga
    17401 aggacgcagc gcagtttgtc ggctaccagg gcgacgccgc tgcgcctgca tccgtgctgc
    17461 tggtgaacaa cggcctgcat atcgatattc agatcgacaa gaataccgcc atcggcaaga
    17521 gcgatgccgc cggcgtggcc gacatcgtgc tggaatcggc gctgtccacc atcctggacc
    17581 tggaagactc ggtggctgcc gtggatgccg acgacaaggt gctgggttac agcaactggc
    17641 tgggcatcct gaaaggaacg ctgacggaaa ccttcgacaa gggcggcaag cagctgaccc
    17701 gcggcctgaa ccccgaccgc gaatatctgg ccggcgacgg caagagcgtg aagctacatg
    17761 gccgctcgct gctttttgtg cgcaacgtcg gacatttgat gacaaatcct gccattcttt
    17821 ggggcaagga agggcgcgag attcccgagg gcatcatgga cgccgtcgtc accacggcca
    17881 ttgccctcca tgatttgaag gggcttgcca cggcaggcat caagaactcg cgcaccggca
    17941 gcgtctacat cgtcaagccc aaaatgcacg gtcccgctga agtgggcttc gccaacgaat
    18001 tgttcggccg cgtcgagcaa ctgctgggcc tggccgagaa caccgtcaaa ctggggatca
    18061 tggatgagga gcgacgaacg tcggtcaacc tgaaggcctg catcgccgcc gctccggcgc
    18121 gcgtggcctt catcaatacc ggcttcctgg atcgcacggg cgacgaaatg cacaccgcca
    18181 tgcaggccgg ccccatgatc cgcaagggcg acatgaagac cagcgcctgg atccaggcct
    18241 acgaaaaaag caatgtgctc gtcggcctgg gcatggggct gcgcggcaag gcgcagatcg
    18301 gcaagggtat gtgggccatg cccgatctga tgaaggccat gctggagcaa aagattgccc
    18361 acccgaaagc cggcgccaac accgcctggg tcccgagccc caccggcgcc accttgcacg
    18421 ccctgcatta ccaccaggtc aaggtgtcgg acatccaaat ccagctggaa aagaccgatg
    18481 ccgatgccga acgcgacaac ctgctcaccg gcctgctgac cgtgcctgtc tccagcaacc
    18541 cgaattggag tacggaagaa aagcagcagg agctggacaa caacatccag ggcatcctgg
    18601 gttacgtggt gcgctgggtg gatcagggcg tgggctgctc caaggtgcca gacatcaacg
    18661 acatcggcct gatggaagac cgcgccacgc tgcgcatcag cagccagcat gtggccaact
    18721 ggctgttgca cggcatcgtc agcgaagggc aggtgcgtgc caccttggag cgcatggccg
    18781 ccaaggtgga tcaacaaaac agcggggatc cgttgtacca gcgcatgcat ggtcgctttg
    18841 accagtccat ggcctaccag gctgcgtgcg agctggtgtt caagggtgtg gagcaaccca
    18901 gcggctacac tgaaccgctg ctgcacgcct ggcgcctcaa ggtcaaggcc gctgcctgat
    18961 gcttcattta atatagctgt ctgcgcttga tacataagtg caaccagcgt attttgacaa
    19021 cagcggcacc caagggtgcc gttgtgcttt tacactgcgg tcatgcctga atccaacagt
    19081 gactgccccc tgtgcggcca gtccaaccaa tgcgccatgg tcgccggcct acccgcgaca
    19141 cgatgctggt gcatgacgca accggtggcg cctgccgcat tgcagcaact accgccagag
    19201 cgtcgcggcc tcgcctgcat ctgccccgaa tgtgcacgcc cgctaccgcc catgcagcaa
    19261 tcgctaccga tatgatcaag gccccttttt tggagaacaa ccatgcccac gtatcacatc
    19321 gaaatgctgg aaggccgcac cgtcgagcaa aaacgtctgt tggctgagga aatcacccgt
    19381 gtctcggtcg aaatcctggg cggatcgccc gattcagtgg acatattgat caccgacgtg
    19441 aagcgcgaaa actgggccac gggcggcaag ctctggctcg aacggcaata agctagcgcc
    19501 acccatgtgc cagctgctcg gcatgaatgc caacactcca accgacgtga ccttcagctt
    19561 cacggggttt gcacagcgtg ctggcgggac agcagaccat accgacggct ggggcatcgc
    19621 cttcttcgag ggccgcggtc tgcgccactt tgtcgatcat gagcgggcca tagactcacc
    19681 ggtggctgag ttgatccggc gctaccccat caagagccgc aacgtcatcg cccatatccg
    19741 caaggcgacc cagggcgtgg tcagcctgga aaactgtcac ccctttgtgc gcgaactatg
    19801 gggccgctac tgggtattcg cccacaacgg cgacctgaag gacttcagac cccgcctgca
    19861 cgcccacttt caccctgtgg gtagcaccga cagcgagcat gtgttttgct ggctgatgca
    19921 ggagttgtcc aaatcccacg ccggcatgcc gcgcatagaa gagctcacgc tcacgctacg
    19981 cgaactggcc gcccgcattg cgccacacgg aactttcaac ttcctgctct ccaacgggca
    20041 ggccttgtgg gcgcatgcct cgacgagtct gtactacatc gagcgccgcc acccgttctc
    20101 gcaagcccag ctatgtgacg aagacatgcg ggtggatttt tctgctctca ccacgcccaa
    20161 ggacaaggtc gccgtggtcg tcaccgcccc gctgaccacc aacgaggaat ggacggcctt
    20221 ccagtcggga gaaatgcgtg tgtttgtgga tggtgaatgc ttggctgaat gaaaccagcg
    20281 ccataaaacc cctgcttgcg atttctatag cccccaaagc aaaacgccct cccactatgt
    20341 aagtgggagg gcggtttggg ctgta
//
LOCUS       DAOMTQ010000041        19905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_333_length_19905_cov_26.641259, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000041 DAOMTQ010000000
VERSION     DAOMTQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 19905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19905
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_19905_cov_26.641259"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK518_05995"
     CDS             complement(<1..276)
                     /locus_tag="PK518_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRM48500.1"
                     /translation="MSTLHKLSAAELLAGYRQRQFSPVEVARDVLEHITRWEPHLAAT
                     WLLRPEAALAQARASEARWLRGAPCGLLDGVPATLKDNIATAGDPTPL"
     gene            complement(285..1769)
                     /locus_tag="PK518_06000"
     CDS             complement(285..1769)
                     /locus_tag="PK518_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48501.1"
                     /translation="MLTRRTALASGALAALATPLPALAQGRKDSVTLALVLEPPGLDP
                     TAGAASSIAEVTLYNLYETLTKIRPDGSVAPLLAESWEVSPDLKSYTFRLRRDARFHN
                     GAPCNAAAVKFSFDRAGGDKSTNKDKRLFAGLSTQVVDEHTVVLLNREIEPDLLFLLG
                     QATAIIVEPKSADSNSTKPVGTGPYQLADWRRGASLTLTAWREWRQAAQIKMRRATFR
                     FIPDPAAQVAALLAGDVDAFPRVTPRAMAPFKANPRFQLIVSGSRAKTILAINNARKP
                     LSDRRVRSAIAAAIDRKAVIQGAADGLGVPIGSHYVPGAFGYVDTTGINPYDPDKARR
                     LLVEAGVKTPLALTMTLPPAPYARQGGEVIAAQLARVGITVKLQNVEWAQWLSGTYGG
                     KNYDLTLISHVEPFDLGNYAKPDYYWGYQSVPFNALYEQIKNAARATDRARLLAEAQR
                     LLAEDAVHAFLYQPQWVTVANKNLRGLWKDMPVFVNDLSALSWA"
     gene            complement(1807..2667)
                     /locus_tag="PK518_06005"
     CDS             complement(1807..2667)
                     /locus_tag="PK518_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM48502.1"
                     /translation="MSTTKSIAVGAISTRATGQNDSNSPLLRVQDLRRDYPLPRTSLL
                     GPRPQVHALRGVSLDIHAGRSLGVVGESGSGKSTLARLVMALDTPSAGSVELLGRDLH
                     RLTACELRAARRDLQMVFQDPYGSLDPRQTVETIVTEPLQALANATKAELRERAAEVL
                     AQVGLRAGDAHKYPHEFSGGQRQRIAIARALITRPRLIVADEPVSALDVSVQAQVLNL
                     LQDLQERHGVSYLLISHDLAVVQQLCDEVLVLYQGQVVERGAPQALFTQPRHPYTQAL
                     VAAVPRMALP"
     gene            complement(2664..3647)
                     /locus_tag="PK518_06010"
     CDS             complement(2664..3647)
                     /locus_tag="PK518_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48503.1"
                     /translation="MASATPLLEVQNLAITLQTQRGAVRAVHDLGFTLARGETVGLIG
                     ESGCGKSLTALALMGLLPDGASVRGSIRFDGQEVLAMPDKTLCQLRGNRMAMVFQEPM
                     SALNPVHSIGRQVAEPLRLHQGLSARQARTQAMELLERVGIARAAERLDAYPHQFSGG
                     QRQRITIAMALACGPDLLIADEPTTALDVTVQRQILELLAGLVAERGMALILISHDLG
                     VIAQSVARTLVMYGGTVVESGPTRAVFGAMAHPYTQGLFAARPRLGTARVAGERLPTI
                     AGSVPDLADLPAGCPFAGRCPRTLDACHAALPPAVALGAGHEARCIRLGEP"
     gene            complement(3647..4498)
                     /locus_tag="PK518_06015"
     CDS             complement(3647..4498)
                     /locus_tag="PK518_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM48504.1"
                     /translation="MSTALLPGFWHRALRQRSFVIGALLTALLLAAAALSLVWTPWPP
                     YDMAMDATLQPPGAAHWLGTDAYGRDITSLLLVGARSSILVGVIAVGIGLSLGAALGL
                     LSAARGGWVEEAIMRLADFTFAFPALLLAIMLTAVFGPGIVNAIIAIGIFYIPTFARV
                     TRASARAVWARDYILAARACGKGPWRITLEHVLPNIASVLIVQATIQFALAILAEAAL
                     SYLGLGTQPPQPSWGRMLAEAQTLMFQAPLLALWPGVAIALAVLGLNLLGDGLRDLLD
                     PRLARQR"
     gene            complement(4495..5457)
                     /locus_tag="PK518_06020"
     CDS             complement(4495..5457)
                     /locus_tag="PK518_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM48505.1"
                     /translation="MAWFVLQRLLTLIATLLGASAVVFWVLEVLPGNAAQVLLGPDAA
                     PEAVAALAAQLGLDQPALTRYGQWIAGLLTGSLGESHAYGAPVFDLIVERLQLTIPLA
                     LLAMAITCALALAAGLYAAARHGRLGDWGVMGLAQIGIAIPNFWFAILLILLFSVQLQ
                     WFSAGGFPGWTEEAGGGVLPALQALLLPAVALAVVQAAILARITRSAVLDVLREDFVR
                     TARAKGLSRRAALWRHVLRNAMIPVVTVMGLQFANLLAGTIVVENVFYLPGLGRLIFQ
                     SIANRDLVVVRNCVMLLAAMVVLVNFVVDLLYAAIDPRVKVHGL"
     gene            complement(5518..6141)
                     /gene="pth"
                     /locus_tag="PK518_06025"
     CDS             complement(5518..6141)
                     /gene="pth"
                     /locus_tag="PK518_06025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRM48506.1"
                     /translation="MIKLFVGLGNPGPEYEATRHNAGFWWIDALARELKLTLVPERAY
                     HGLMARTSIAGHSVWLLQPQTFMNLSGKSVAALARFFKIQPEEILVVHDELDLSPGQA
                     KLKRGGSHAGHNGLRDIHAQLGSSDYWRLRIGIGHPGVKGEVIHWVLKKPAPDQRQLI
                     EDSIAHSLKAYPTMLAGDMDKATLLVHTTKPPRPKPPRPSAAPDGAV"
     gene            complement(6241..6864)
                     /locus_tag="PK518_06030"
     CDS             complement(6241..6864)
                     /locus_tag="PK518_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRM48507.1"
                     /translation="MQFVAFERAKQGTGASRRLRISGKAPGIVYGGAAEPQMIEIDHN
                     ALWHALKKEAFHSSILDMELNGQTSKVLLRDVQYHPFKQQVLHVDFQRVDEKTRVHLK
                     VPLHYEGLEGSQAVKVEGCTVTQLIHEVDVMCMPSQLPEFLTVDLSALTSKSVLGLQS
                     LKVPHGVQLVVRGSNKNPALVSIKLPEVVVEAAAAAPAAAPAKKGKK"
     gene            complement(6962..7921)
                     /locus_tag="PK518_06035"
     CDS             complement(6962..7921)
                     /locus_tag="PK518_06035"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRM48508.1"
                     /translation="MQTNSQDFVVFTGNANSALAAEIAKHLGTTLGAVDVGRFSDGEV
                     TVEIKQSVRARDVFVVQPTCAPTNENLMELLIMVDALKRASAERITAVIPYFGYARQD
                     RRPRSTRVPISAKVVANMLQAVGVARVLTMDLHADQIQGFFDIPVDNIYASPVLLGDL
                     NQKKYENLMVVSPDVGGVVRARALAKQLGCDLAIIDKRRPKANVSEVMHVIGDIEGRN
                     CVIMDDMIDTAGTLVKAAEVLKERGANNVYAYCTHPIFSGPAIDRITNGAALDEVVVT
                     NTIPLSDNAKGCSKIRQLSVAPLIAETIQRISTGESVSSLFSD"
     gene            complement(7969..8045)
                     /locus_tag="PK518_06040"
     tRNA            complement(7969..8045)
                     /locus_tag="PK518_06040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8009..8011),aa:Gln,seq:ttg)
     gene            complement(8071..8937)
                     /gene="ispE"
                     /locus_tag="PK518_06045"
     CDS             complement(8071..8937)
                     /gene="ispE"
                     /locus_tag="PK518_06045"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRM48509.1"
                     /translation="MQALYDVPAPAKLNLFLHITGRRPDGYHLLQSVFMLIDWCDTLH
                     FDLRQDGGVTREDLTQPLPPDDLVLRAARALQAATGCRLGAHIGVHKRVPAQAGLGGG
                     SSDAASTLLALNRLWKLQLPRSQLQAIGLTLGADVPFFLCGGNAWVEGIGDIIAPLEL
                     EHALPGARFAVVKPAAGLETKAIFSHPLLKRDCASATIQGFAAAHYQFGSNALQPVAQ
                     TLCPEIGQAIDWLASKGLQGRMTGSGSAVFAPIAQSVDLQDAPPTWQVKMCNNLRLHP
                     LTEWASDGEIKG"
     gene            complement(8953..9459)
                     /locus_tag="PK518_06050"
     CDS             complement(8953..9459)
                     /locus_tag="PK518_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HRM48510.1"
                     /translation="MPGAVSERPQRGRLLLAALGAALLGACAQPPRPASGAADHWSGR
                     LALQIEDASAQSFSAGFELQGSPDAGALTLFNPLGNIIAELQWTPGRAILNNGGEQRQ
                     SASLQTLVQALTGSELPIAALFGWLKGEPVQAAGWQADLSALDKGRLTATRHQPAPQA
                     VLRVILSL"
     gene            complement(9462..11240)
                     /locus_tag="PK518_06055"
     CDS             complement(9462..11240)
                     /locus_tag="PK518_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM48511.1"
                     /translation="MDYYGPMELLQRFRFAAFVLATAAATWPALAQTQAHSTPESEEE
                     DSDDANAALTAELFYELLLGEMTASSGDPGTGYNLILDAARRSRDGRLYRRATEIALQ
                     ARSGESALAAARAWQQALPQSREANRYLLRILVALNRIGESAAPLRQELANAPTRDRI
                     ANIRALPLLYARASDKALAARVVRQALEDELSHPAAGPAAWVAIGRMYFAADDKARTL
                     EAAGNALALDANDEGAAMLALQLMEAGVAEAEPLLSRYLTGKPLPEVRLAYARMLLES
                     QRLSDAQAQVQAVTREHPDMAEAWLIQATLDLQTGRLAEAESAVQQFTRLQDGLPASE
                     QRKHALTQSYLLQAQIAEKRGNFAQAESWLNRIEDAAERLPVQSRRATLLARQGRLAE
                     ARALIRAMPAKGADDERMKLQAEVQLLREMGQYQQAYELQAKVVAMAPQDNDLVYDQA
                     MLADKAGKPQEMERLLRSIIARKPDYHHALNALGFSLADRGVRLQEARTLIMKALEYA
                     PEDPFITDSLGWVEFRLGNRKEALAILERAFKRQQDVEIAAHLGEVLWSLGQRERALA
                     IWREGLRINQDNSTLKDTLKRLGAQP"
     gene            11272..12087
                     /gene="mutM"
                     /locus_tag="PK518_06060"
     CDS             11272..12087
                     /gene="mutM"
                     /locus_tag="PK518_06060"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRM48512.1"
                     /translation="MPELPEVEVTRRSFADAIAGATIAALVLGKPLRWPLGVAPQALV
                     GQRVVAVRRRGKYLLLDLQDGLLLIHLGMSGSLRFASGLPQRGPHDHFDMETDRGTLR
                     LHDPRRFGAVVWAASEEHPHARKLLAGLGVEPLGEDFDFAAFHAGLRASRMPIKQLLL
                     AGRLVVGVGNIYTSEVLFLAGIRPTTRASSIGPQRARRLHGAIREVLAQAVQLGGSTL
                     RDFSSADGSAGHFQLQAHVYGREGQPCRLCATPVRMLRQGQRSSYFCPRCQRP"
     gene            12188..14188
                     /locus_tag="PK518_06065"
     CDS             12188..14188
                     /locus_tag="PK518_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRM48513.1"
                     /translation="MGPSFNQRFDQHGTWRRMFAHQLAQLGQWMGANDLLDAGVQERL
                     ERLEQQVRSDKVTVAFVGEFSRGKSELINAIFFAGYGRRIMPASAGRTTMCPTELGYD
                     GAIEPCLRLLPIETRLQASGVAEWRLRPGSWVRLALNMDDPEQMARTLEKLSEMRSVP
                     LQEARALGFWSDEADGAGLTPDADGMVQVPAWRHAIVNLPHPLLKQGLVILDTPGLNA
                     VGAEPELTINLIPQAHAVVFILAADTGVTRSDLAIWREHLGAAEDAVDARLMVLNKID
                     TLWDELTPQEQVQAQIERQCAGAAQLLGVQPDQVVAVSAQKGLLAKIRCDDDLLQASG
                     LPQLEVILGTGIMGRRQAILRATVGSGVASLEAEIRRLLHFRRRDLDEQMLELRSLRG
                     KNASVIDAMRGRIEVEQQEFEASTTKIQAVRAVHMKMLRDLFQRLGPRGLRVHLAALA
                     EALQHPGLKLGVRKVYGEAFDQVRGAIDAAQAAGGEIQAMLGGTFRQLNTDFGFSLQV
                     PQAPTLAAHIREITAIERRHLQYIGLASTLRLAQPEHGPRLLHALTQRLRTVFEAVAN
                     ELELWSNSASSQLEAQMRERRHSFARRIEAVNRIQDAASGLMERIAEIEDAERNLAVL
                     EQRLLELTAQLAPQVLVDEAAAAPDMAQPQTEPLPLTRVATA"
     gene            14185..15243
                     /gene="mutY"
                     /locus_tag="PK518_06070"
     CDS             14185..15243
                     /gene="mutY"
                     /locus_tag="PK518_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRM48514.1"
                     /translation="MSGAPVEIAAQVVRWQAEHGRNQLPWQNTRDAYRVWLSEIMLQQ
                     TQVVTVLEYYARFLARFPDVRQLAAAPQDEVLALWSGLGYYSRARNLHRCAQIVVAEH
                     GGEFPRTVHELAALPGIGRSTAGAIAAFCFGVRAPILDANVRRVLTRVLGFGADLAEV
                     RNERALWSLAEALLPDTDLASTMPRYTQGLMDLGAGICLPRSPCCMLCPLQPVCVAGR
                     AGDAERYPVRTRKLKRRAEAWWLLLRQDRAGRLWLERRAPVGIWAGLYCPPVYASRRD
                     LDEALAPAVRSEVQDLPAFTHVLTHRDLHLHPVLARCDMAAPAGQCSEALVADWFTPE
                     LWQGLGLPAPVRRLLDAL"
     gene            complement(15348..16226)
                     /gene="rapZ"
                     /locus_tag="PK518_06075"
     CDS             complement(15348..16226)
                     /gene="rapZ"
                     /locus_tag="PK518_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRM48515.1"
                     /translation="MSLEIVVITGMSGSGKSLALRALEDSGYYCVDNLPPELLASFVA
                     LEHSHRSQRVAVAMDVRSATALPLVPQQLNRLRETGVQVRSLFLDASTGTLVRRFSET
                     RRRHPLSQNELPETRLALVQTIELERQLLAELREQSYVIDTSVLRPAQLLTYVKELLA
                     VKPGQLTLVFESFAFKHGVPFDSDYVFDVRMLPNPHYEPTLRALTGRDAPVIEYLRQQ
                     PEVERMQSDISQFLEHWLDALAQNHRSYVTVAIGCTGGQHRSVYLAEQLSAQFASHWS
                     TLCRHRELDGIRHGTT"
     gene            complement(16230..17900)
                     /gene="recN"
                     /locus_tag="PK518_06080"
     CDS             complement(16230..17900)
                     /gene="recN"
                     /locus_tag="PK518_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRM48516.1"
                     /translation="MALRRIALRDFVIVQALDLDLQTGFTVLTGETGAGKSILIDALQ
                     LLLGARADSGVIREGAERTDISAEFDIGPAALTDWLDEAGFPHDELLLRRTIDLQGKS
                     RAWINGLPATATQMRALGEHLLDIHGQHAWQSLTRPDAVRGLLDAYAGVQAQPLAALW
                     QTWREAQKALLHARAAQDTLQQERERLQWQITEVDKLAPSDDEWDELNARHARLSNAQ
                     ALLDNAQGALGALQGDDAGGALSALAQAQHLLQDYEHVDASFRAFNEVLSACIAQAGD
                     VAHSLQAYLRHAEPDPELLEQLDARLAQWMQLARRYKRQPPELPALLAGWKEALRQLD
                     SATDLAALEAAEQTHAAAYQKAARALSQKRAKAAPQLAQAITQAMQGLGMTGGRFEVQ
                     VDKAAEPGPQGMDQVAFLVASHPGMTPRPVGKVASGGELSRIALAISVTTSELGEAPT
                     LIFDEVDSGVGGAVAETVGRLMQQLGRARQVLAVTHLPQVAACADQHLVVSKQRSAAG
                     TTSAVAVADADERVAEIARMLGGQQQSPTTMAHAREMLAGARPATTTG"
     gene            complement(17903..18799)
                     /locus_tag="PK518_06085"
     CDS             complement(17903..18799)
                     /locus_tag="PK518_06085"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRM48517.1"
                     /translation="MKPIFRRVAVIGKYREPVAGAPPASSRQIIEGIARLIIQEGGEA
                     TLEAETAATLGITGYPQLDVDAIGGHCDLCVVVGGDGTMLGVGRRLAAYGTPLVGINK
                     GRLGFITDIPLQSYETSLTAILHGEYEEDVRPLMQARVVRGGECVFEALALNDVVVNR
                     GSTAGMVELRIEVDGVFVSNQRADGLIVASPTGSTAYSLSAGGPMLHPSIPGWVLVPI
                     APHTLSNRPIVLSDSSEIAIEVAGGRGISANFDMQSLASLQRGDRVLVRRSAHRVCFL
                     HPRGWSYFSTLRKKLRWNEGGS"
     gene            18828..19832
                     /gene="hrcA"
                     /locus_tag="PK518_06090"
     CDS             18828..19832
                     /gene="hrcA"
                     /locus_tag="PK518_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRM48518.1"
                     /translation="MLDDRSKLLLKALVERYIADGQPVGSRTLSRASGLELSPATIRN
                     VMADLEELGLIASPHTSAGRIPTARGYRLFVDTMLTVQQEQLPALQLMPEQPQKVIAN
                     AAQLLSNLSQFVGVVMAPRRASVFRHIEFLRLSERRLLVIIVSPDGDVQNRVIFTEAD
                     YTPSQLVEAANFLNAHYAGLTMEQVRERLKLEVDKLRGEIATLMQAAVNVGSQAMSES
                     QDEVVFSGERNLLAVSDFSGDMGHLRRAFDLFEQKTQILRLLDISSRAEGVRIFIGGE
                     SQVVPFEELSVVSAPYEVDGQVVGTLGVIGPTRMPYDRMIQIVDITSKLVSNALSHHK
                     "
BASE COUNT         3069 a         6521 c         7043 g         3272 t
ORIGIN      
        1 cagcggcgtg gggtcgccgg cggtggcgat gttgtccttg agcgtggcgg gcacgccgtc
       61 cagcaggcca cagggcgcgc cgcgcagcca gcgtgcctcg ctggcgcgcg cctgggccag
      121 ggcggcctcg gggcgcagca gccaggtggc ggccaggtgc ggctcccagc gggtgatgtg
      181 ctccagcaca tcacgcgcca cctccacggg cgagaactgg cgttggcggt agccggccag
      241 cagctcggcg gcggacagtt tgtgcagcgt gctcatgccg gtcctcatgc ccacgacagc
      301 gccgacaggt cgttgacgaa gaccggcatg tccttccaca gcccgcgcag gttcttgttc
      361 gccaccgtca cccattgcgg ctggtacaga aaggcgtgca ccgcgtcctc ggccagcagg
      421 cgctgggctt cggccagcag gcgtgcgcgg tcggtggcgc gcgcggcgtt cttgatctgc
      481 tcgtacaggg cgttgaaggg caccgactgg tagccccagt agtagtcggg cttggcgtag
      541 ttgcccaggt cgaagggctc gacatgcgag atcagcgtca ggtcgtagtt cttgccgcca
      601 taggtgcccg acagccactg cgcccattcg acgttctgca gcttcacggt gatgcccacc
      661 ctggccagct gcgcggcgat gacctcgccg ccctggcgcg cgtagggcgc gggcggcagg
      721 gtcatggtga gtgccaaagg cgtcttcacg ccggcttcga ccagcaggcg cctggccttg
      781 tccgggtcgt aggggttgat gcccgtggtg tccacatagc cgaaggcgcc cggcacatag
      841 tggctgccga tcggaacgcc caggccgtcg gccgcgccct ggatgacggc cttgcggtct
      901 atggccgcgg cgatggcgct gcgcacgcgc cggtcgctca agggctttct cgcgttgttg
      961 atggccagga tggtcttggc gcgcgagccg ctgacgatga gctgaaagcg cgggttggcc
     1021 ttgaacggcg ccatggcgcg cggcgtgacg cgcgggaagg cgtccacgtc gcccgccagc
     1081 agcgccgcca cctgggccgc cgggtcggga atgaagcgga aggtggcgcg gcgcatcttg
     1141 atctgcgcgg cctggcgcca ctcgcgccag gcggtgagcg tgagcgaggc gccgcggcgc
     1201 cagtcggcca gttggtaggg gccggtgccc acgggcttgg tggagttgct gtcggcgctc
     1261 ttgggctcga cgatgatggc cgtggcctgg cccagcagga acagcaggtc tggctctatc
     1321 tcccggttga gcagcaccac cgtgtgctcg tccaccacct gggtggacag gccggcgaac
     1381 aggcgcttgt ccttgttggt gctcttgtcg ccgccggcgc ggtcgaagga gaacttgacg
     1441 gccgcggcgt tgcacggcgc gccgttgtga aagcgggcgt cgcggcgcag gcggaaggtg
     1501 tagctcttca ggtcgggcga gacctcccag ctctcggcca gcaggggcgc gacgctgccg
     1561 tcggggcgga tcttggtcag cgtctcgtag aggttgtaga gcgtgacctc ggcgatcgat
     1621 gaggccgcgc cggccgtggg atcgagcccc ggcggctcca gcaccagcgc cagggtgacg
     1681 ctgtccttgc ggccctgggc cagtgccggc agcggcgtgg ccagcgcggc cagggcgccg
     1741 gaggcgaggg cggtgcggcg ggtgagcatg ggctgaagtc tccttggggt gaatgcggtg
     1801 tttttactac ggcagggcca tgcgcggcac ggccgccacc agcgcctggg tataggggtg
     1861 ccgcggctgg gtgaacagcg cctggggcgc gccgcgctcc actacctggc cctggtacag
     1921 caccagcacc tcgtcgcaca gctgctgcac caccgccagg tcgtggctga tcagcagata
     1981 gctcacgcca tggcgctcct gcagatcctg cagcaaattc aatacctggg cctgcaccga
     2041 gacgtcgagc gcgctcaccg gctcgtcggc gacgatcagg cgcgggcgcg tgatcagggc
     2101 gcgggcgatg gcgatgcgct ggcgctggcc gccggaaaat tcatgcgggt acttgtgcgc
     2161 gtcgcccgcg cgcaggccga cctgggccag cacctcggcg gcgcgctcgc gcagctcggc
     2221 ctttgtggcg ttggccagcg cctgcagcgg ctcggtgact atggtctcca ccgtctggcg
     2281 cgggtccagc gagccgtagg ggtcctgaaa caccatctgc aggtcgcgcc gcgcggcacg
     2341 cagttcgcac gcggtcaggc ggtgcaggtc gcggcccagc agctccacgc tgccggcgct
     2401 gggcgtgtcg agcgccatca ccagccgcgc cagcgtggac ttgcccgagc ccgattcgcc
     2461 caccaccccc aggctgcgcc cggcgtggat gtcaaggctc acgccgcgca gcgcgtgcac
     2521 ctgggggcgc ggccctagca ggctggtgcg cggcagcggg tagtcgcgcc gcaggtcttg
     2581 cacgcgcagc agcggactat ttgagtcatt ttggcctgta gcgcgcgtgg atattgcgcc
     2641 aacagctatt gattttgtgg tgctcatggc tcccccaggc gtatgcagcg cgcctcatgg
     2701 cccgcgccca gggccacggc cgggggcagg gcggcgtggc aagcgtccag ggtgcgcggg
     2761 cagcgcccgg caaacgggca gcccgcgggc aggtcggcca gatcgggcac gctgccggcg
     2821 atggtcggca ggcgctcgcc ggcaacgcgc gccgtgccca gccggggacg ggcggcgaac
     2881 aggccctggg tgtaggggtg ggccattgcg ccaaaaacgg cgcgcgtggg gccggactcg
     2941 acgacggtgc cgccgtacat caccagcgtg cgcgccacgc tctgcgcgat cacgcccagg
     3001 tcgtgcgaga tcaatatcag tgccatgccg cgctcggcca ccaggccggc gagcagctcc
     3061 aggatctggc gctgcacggt cacgtccagc gctgtcgtgg gctcgtcggc gatgagtagg
     3121 tcgggcccgc aggccagcgc catggcgatg gtgatgcgct ggcgctgccc gcccgagaac
     3181 tggtgcgggt aggcgtccag gcgctcggcc gcccgggcaa tgcccacgcg ctccagcagc
     3241 tccatggcct gggtgcgcgc ctgcctggcc gacaggccct ggtgcaggcg cagcggctcc
     3301 gccacctggc ggccaatgct gtgcacgggg ttgagcgcgc tcatcggttc ctgaaacacc
     3361 atggccatgc ggttgccgcg cagctggcac agcgtcttgt cgggcatggc cagcacctcc
     3421 tggccgtcaa agcggatgct gccccgcacg ctggcgccat cgggcagcag gcccatcagg
     3481 gccagtgcgg tgagggattt gccgcagccc gattcgccta tcagccccac ggtctcgccg
     3541 cgcgccagcg tgaaacccag gtcatgcacg gcgcgcaccg cgccgcgctg tgtttgcagg
     3601 gtgatggcga ggttttgcac ctctagcagt ggtgttgcgc tggccatcat ctttgtcgcg
     3661 ccaggcgcgg gtccagcagg tcgcgcaggc cgtcgcccag caggttcagg ccgagcacgg
     3721 ccagcgcaat cgccacaccc ggccacaagg ccagcagcgg cgcctgaaac atcagcgtct
     3781 gggcctcggc cagcatgcgc ccccaggacg gctggggcgg ctgcgtgccc aggcccaggt
     3841 acgaaagtgc cgcctcggcg aggatggcca gcgcgaactg gatcgtggcc tgcacgatca
     3901 gcaccgaggc gatgttgggc agcacatgct cgagcgtgat gcgccacggc cccttgccgc
     3961 aggcgcgcgc cgccaatatg tagtcgcgcg cccagacggc ccgggccgag gcgcgcgtga
     4021 cgcgcgcgaa cgtcggtatg tagaagatgc cgatggcgat gatggcgttg acgatgcccg
     4081 ggccgaacac ggccgtgagc atgatggcca gcagcagggc tggaaaggca aaggtgaagt
     4141 ctgccaggcg catgatggcc tcctccaccc agccgccgcg cgcggccgac agcaggccca
     4201 gcgccgcgcc cagtgacagg cctatgccca cggcgatcac gccgaccagg atggagctgc
     4261 gcgcgcccac cagcagcagc gaggtgatgt cgcggccgta ggcgtcggtg cccagccagt
     4321 gcgcggcgcc gggcggctgc agggttgcgt ccatggccat gtcgtagggc ggccagggcg
     4381 tccagaccag ggacagggcg gcggcggcca gcagcagcgc cgtcagcagc gcgccgatca
     4441 cgaagctgcg ctgacgcagc gcgcggtgcc agaagccggg caggagggcg gtgctcacag
     4501 tccgtgaacc ttcacgcgcg ggtctatggc cgcgtagagc agatcgacga cgaagttgac
     4561 cagcaccacc atggccgcca gcagcatcac gcagttgcgc accaccacca ggtcgcggtt
     4621 ggcaatcgac tggaagatca gccgccccag gccgggcagg tagaacacgt tctcgaccac
     4681 gatggtgccg gccagcaggt tggcgaactg caggcccatc accgtgacca cggggatcat
     4741 ggcgttgcgc agcacatggc gccacagggc cgcgcgccgg gacaggccct tggcgcgcgc
     4801 ggtgcgcacg aagtcctcgc gcagcacgtc gagcacggcc gagcgcgtga tgcgcgccag
     4861 gatcgccgcc tgcaccaccg ccagcgccac ggccggcagc agcagggcct gcagcgccgg
     4921 cagcacgccg ccgccggcct cttcggtcca gcccggaaag ccgccggccg agaaccactg
     4981 cagctgcacc gagaacagca ggatcagcag gatggcgaac cagaagttgg gaatggcaat
     5041 gccgatctgc gccaggccca tcacgcccca gtcgcccagg cggccgtggc gcgcggccgc
     5101 gtacaggccg gcggccaggg ccagggcgca ggtgatggcc atggccagca gggccagcgg
     5161 aatggtcagc tgcaggcgct cgacgatgag atcgaacacc ggcgcgccgt aggcgtggct
     5221 ctcgcccagg ctgccggtca gcaggccggc gatccactgg ccgtagcgcg tgagggccgg
     5281 ctggtccagc cccagttgcg cggccagggc ggccacggcc tcgggtgcgg cgtcgggacc
     5341 cagcagcacc tgggcggcgt tgccgggcag cacctccagc acccagaaga ccacggccga
     5401 ggcgcccagc agcgtggcga tcagggtcag caggcgttgc aggacgaacc aggccatatg
     5461 atggaaatgt ggctgcaggg cccgtctatc atgcgcttgt agctatgaaa aacatagtta
     5521 aacggcgccg tcgggtgcgg cggagggccg gggtggcttg gggcgcggcg gcttggtggt
     5581 gtgcaccagc agcgtggcct tgtccatgtc gccggccagc atggtggggt aggccttgag
     5641 cgaatgggcg atgctgtcct cgatcagctg gcgttgatcg ggcgcgggct ttttcagcac
     5701 ccagtggatc acctcgccct tgacgcccgg gtggcctatg ccgatgcgca ggcgccagta
     5761 gtcgctgctg cccagctggg cgtggatgtc gcgcaggccg ttgtggccgg cgtggctgcc
     5821 gccgcgcttg agcttggcct ggccgggtga caagtccagc tcgtcatgga cgacgaggat
     5881 ctcctcgggc tggatcttga agaagcgcgc cagcgccgcc accgacttgc ccgacaggtt
     5941 catgaaggtc tgcggctgca gcagccagac gctgtggccg gcgatgctgg tgcgcgccat
     6001 caggccgtgg taggcgcgct cgggcaccag cgtcagcttc agctcgcgcg ccagtgcatc
     6061 tatccaccaa aagccggcgt tgtggcgcgt ggcctcgtat tccgggccag ggttgcccag
     6121 gcccacaaac agtttgatca tggctgcgat tatctggtgc ccacaaatgc caacggccca
     6181 cgcgaggtgg gccgttgtgc aagccagccg cctgcactgc aggcggtgcg caagaagtga
     6241 ttacttcttg cccttcttgg cgggagccgc tgccggtgcg gcggcggccg cttcgaccac
     6301 cacttcgggc agcttgatgg acaccagggc ggggttcttg ttcgagccac gcaccaccag
     6361 ctgcacgcca tggggcacct tcagcgattg caggcccaga accgacttgg aggtcagagc
     6421 gctcaggtcc acggtcagga actcgggcag ctgcgagggc atgcacatca cgtccacttc
     6481 gtggatcaac tgggtgacgg tgcagccttc aaccttcacg gcctgcgagc cttccaggcc
     6541 ttcgtagtgc agcggcacct tcaggtgcac gcgggtcttc tcgtccacgc gctggaagtc
     6601 cacatgcagc acctgctgct tgaaggggtg gtactgcacg tcacgcagca gcaccttgct
     6661 ggtctggccg ttgagttcca tgtccaggat gctggagtgg aaggcttcct tcttcagggc
     6721 gtgccacagg gcgttgtgat cgatctcgat catctggggt tcggcggcgc caccgtagac
     6781 gatgccgggg gccttgccgg aaatgcgcag acggcggctc gcacccgtac cttgcttggc
     6841 gcgctcaaaa gcgacgaatt gcatgttgct aactcctgat ggggccagcc gcgaccagcg
     6901 ggcccactgt tgaaaaagac cccatgcccg acggacatga ggcctgcttg gtgttcccgg
     6961 atcagtccga gaacaggctc gataccgact cgcccgtgga aatgcgctga atcgtctcgg
     7021 cgatcagcgg cgccacggag agctggcgaa tcttgctgca ccccttggcg ttgtcgctca
     7081 aggggatggt gttggtgacg accacctcgt ccagcgccgc gccgttggtg atgcgatcga
     7141 tggccgggcc ggagaagatg gggtgcgtgc aataggcgta cacgttgttg gcgccgcgtt
     7201 ccttgagcac ctcggccgct ttcaccagcg tgccggcggt gtcgatcatg tcgtccatga
     7261 tcacgcagtt gcggccttcg atgtcgccga tgacatgcat gacctcggac acattcgcct
     7321 tggggcggcg cttgtcgatg atggccaggt cgcagccaag ctgcttggcc agcgcgcgcg
     7381 cgcgcaccac gccacccacg tcggggctga cgaccatcag gttctcgtac ttcttctggt
     7441 tcaggtcgcc cagcagcacc ggcgaggcgt agatgttgtc cacgggaatg tcgaagaaac
     7501 cctggatctg gtcggcgtgc aagtccatgg tcagcacccg ggccacgccc acggcctgca
     7561 gcatgttggc caccaccttg gccgagatgg gcacgcgcgt ggagcgcgga cggcgatcct
     7621 ggcgggcata gccaaaatag ggaatcacgg cggtgatgcg ttcggccgag gcgcgcttga
     7681 gcgcatcgac catgatcagc agctccatca ggttttcgtt ggtcggcgcg caggtcggct
     7741 ggaccacgaa aacatcgcgt gcgcgcacgc tttgcttgat ctcgaccgtc acctcaccat
     7801 cggagaagcg gccgacatcg acggcgccca gggtggtgcc caggtgtttg gcgatttcgg
     7861 cagccaaagc ggaattggcg ttgcccgtga acaccacgaa atcttgggag ttggtttgca
     7921 tgagtgactc agcagtctga atgaatatcc agcgcgggca atgggttttg gcaggggagg
     7981 aaggattcga accctcgcat gccggaatca aaatccggtg ccttaaccga cttggctact
     8041 cccctacaca ggtcgattgc cgtggggcaa tcaacccttg atctctccat ctgaagccca
     8101 ttccgtcaac ggatgaagcc gcagattatt gcacattttg acttgccatg ttggcggcgc
     8161 gtcctgcaaa tcaaccgact gtgcaattgg tgcaaacacc gcgcttccag aaccggtcat
     8221 gcgcccttgc aagcccttgg atgccagcca gtcaatggcc tggccaatct cggggcacag
     8281 agtttgcgct accggctgca aggcgttgct accaaactgg tagtgtgctg cagcaaagcc
     8341 ttgaattgta gcagaagcgc aatcgcgttt tagcagtgga tgcgaaaaaa tggccttggt
     8401 ctccagcccg gccgcgggct tgacgactgc gaagcgcgcg cctggcaagg cgtgctccag
     8461 ttcaaggggg gcaatgatgt cgcctatgcc ctccacccag gcgttgccgc cgcacagaaa
     8521 gaagggcacg tccgcgccca gcgtcaggcc tatggcctgc aactggctgc gcggcagttg
     8581 cagcttccac aggcggttga gtgccagcag cgtgctggcc gcgtccgacg agccgccgcc
     8641 cagcccggcc tgggccggca cgcgtttgtg cacgccgata tgcgcgccca ggcggcagcc
     8701 ggtggcagcc tgtagcgcgc gtgccgcgcg cagcaccaag tcgtcaggcg gcaggggttg
     8761 tgtgaggtcc tcgcgcgtga cgccgccgtc ctggcgcaaa tcgaaatgca gggtgtcgca
     8821 ccagtcgatg agcatgaaca ccgattgcag caggtgatag ccatcgggcc gcctgccggt
     8881 gatgtgcaga aacagattca gcttggccgg tgccggcacg tcgtacaggg cttgcatgcg
     8941 gagggcaggt gttcaaagac tcaggatcac gcgcagcacg gcctgcggcg cgggctggtg
     9001 gcgcgtggcg gtcaggcggc ccttgtccag tgcgctcaag tccgcctgcc agccggcagc
     9061 ctgcactggt tcgcccttga gccagccaaa cagcgcggcg atgggcagtt cgctgcccgt
     9121 cagcgcctgc accagggtct gcagcgaggc ggattgccgt tgctcgccgc cattgttgag
     9181 aatggcgcgc ccaggcgtcc attgcagctc ggcgatgatg ttgcccagcg ggttgaacag
     9241 ggtcagcgct cccgcatcgg gactgccctg gagttcaaag ccggcgctga atgactgtgc
     9301 gctggcgtcc tcgatctgca gggccaggcg cccgctccag tggtcggccg cgccgctggc
     9361 agggcggggc ggctgggcgc aggcgccgag cagtgcggcg cccagtgcgg cgagcagcag
     9421 ccggccgcgt tgcgggcgtt cgctgacggc tcctggcaag gtcaaggctg cgctcccagg
     9481 cgcttgagcg tgtccttgag cgtgctgttg tcctggttga tgcgcagacc ctcgcgccag
     9541 atggccaggg cgcgctcgcg ctggcccagg ctccacagca cctcgcccag gtgggcggcg
     9601 atctccacgt cctgctgtct cttgaaggcg cgctccagaa tggccagcgc ctccttgcgg
     9661 ttgcccaggc gaaactccac ccagcccagg ctgtcggtga tgaagggatc ttcgggtgcg
     9721 tattccaggg ccttcatgat cagcgtcctg gcttcctgca gtcgcacgcc gcgatcggca
     9781 agcgaaaacc caagggcgtt gagtgcatgg tggtagtccg gcttgcgcgc gatgatgctg
     9841 cgcagcagcc gctccatctc ctggggcttg ccggccttgt cggccagcat ggcctggtca
     9901 tagaccaggt cgttgtcttg cggcgccatg gccacaacct tggcctgcag ctcataggcc
     9961 tgctggtact ggcccatttc gcgcagcagc tgcacctcgg cctggagctt catgcgctcg
    10021 tcatcggcgc ccttggcggg catggcgcgg atcaatgcgc gtgcctcggc caggcggccc
    10081 tggcgcgcca gcagcgtggc gcgccggctc tggacgggca ggcgctcggc cgcatcctcg
    10141 atgcggttca gccagctctc ggcctgggca aagttgccgc gcttttcggc gatctgggcc
    10201 tgcagcaggt aggactgggt cagggcgtgt ttgcgttgct cgctggcggg cagcccgtcc
    10261 tgcagcctgg tgaactgctg cacggcagac tcggcctcgg ccaggcggcc ggtctgcaga
    10321 tccagcgtgg cctggatgag ccaggcctcg gccatgtcgg gatgctcgcg cgtgacggcc
    10381 tggacctggg cctgggcgtc gctcaggcgc tgcgactcga gcagcatgcg cgcataggcc
    10441 aggcgcacct cgggcagggg tttgcctgtc aggtagcgcg acagcagggg ctcggcctct
    10501 gcgacgccgg cctccatcag ctgcagcgcc agcatggccg cgccttcgtc gttggcgtcc
    10561 agtgccagcg cattgccggc ggcctccagt gtgcgggcct tgtcatccgc ggcgaaatac
    10621 atgcgcccga tggcgaccca ggcggccggc ccggcggctg gatgactcag ctcgtcctcc
    10681 agggcctggc gcaccacgcg tgcggccagc gccttgtcgc tggcgcgcgc atacagcagg
    10741 ggcagggcgc ggatgttggc gatccggtcg cgcgtcgggg cgttggccag ctcctggcgc
    10801 aatggcgcgg cactttcgcc tatgcggttg agtgccacca ggatgcgcag caggtagcga
    10861 ttggcctcgc gcgactgcgg cagcgcctgt tgccaggcac gggccgcggc cagggcagat
    10921 tcgcccgagc gtgcctgcag cgcgatctcg gtggcgcggc ggtacagccg gccatcgcgg
    10981 ctgcgccggg cagcgtccag tatcaggttg tagcccgtgc cggggtcgcc cgagctggcc
    11041 gtcatctcac cgagcagcag ctcgtaaaac agctccgccg tgagggcggc gttggcgtcg
    11101 tcgctgtcct cttcttcgga ttctggcgtg gagtgggcct gggtctgggc cagtgccggc
    11161 catgtggctg ccgcggtcgc aagaacgaag gcggcaaacc tgaaacgctg tagtagctcc
    11221 atcggcccat aataatccat gggtctggga cttttttgaa cgccaacaag catgcctgag
    11281 ctacccgagg tcgaggtcac aaggcgcagt tttgccgacg cgattgcggg ggcaacgata
    11341 gctgcgctgg tgttgggcaa gccgctgcgc tggcccctgg gggtggcgcc gcaggccctg
    11401 gtggggcagc gcgttgtggc ggtgcgccgg cgcggcaagt atctgctgct ggatctgcag
    11461 gacgggctgt tgctgatcca cctgggcatg tcgggcagcc tgcgctttgc aagcggcctg
    11521 ccgcagcggg ggccgcacga ccatttcgac atggagaccg atcgcggcac cctgcggctg
    11581 catgacccgc gccgctttgg cgcggtggtc tgggccgcga gcgaagagca tccgcatgcg
    11641 cgcaagctgc tcgcgggcct gggagtggag cccctgggcg aagactttga cttcgccgcc
    11701 tttcatgccg ggctgcgtgc cagccgcatg cccatcaagc agctgctgct ggcgggcagg
    11761 ctggtcgtgg gggtgggcaa tatctatacg tcggaggtgc tgttcctggc cggcatccgg
    11821 ccgacgacgc gcgccagcag catagggccg cagcgcgcgc ggcgcctgca tggcgccatt
    11881 cgcgaggtgc tggcgcaggc cgtgcagctg ggcggcagca cgctgcggga tttttccagc
    11941 gccgacggca gcgccgggca tttccagctg caggcccatg tctacgggcg cgaaggccag
    12001 ccgtgcaggc tgtgcgcaac ccctgtgcgc atgttgcgcc agggtcagcg cagcagctat
    12061 ttttgcccgc gttgccagcg tccttaaggc gtcgccaaca gcctgcggcc atggggcgcg
    12121 gtgctatatt gcaccttccc gcccccgagt gggcgctcgc attgacgcat ggatacccgg
    12181 gagcaacgtg gggccctcat tcaaccaacg ctttgatcag catggcacct ggcggcgcat
    12241 gtttgcgcac cagctggcgc agctggggca gtggatgggc gcgaacgatc tgttggatgc
    12301 cggggtgcag gagcgccttg agcgcctgga gcagcaggtg cgcagcgaca aggtcaccgt
    12361 ggccttcgtg ggcgagttct cgcgcggcaa gtccgagctc atcaatgcca tcttttttgc
    12421 cggctacggg cggcgcatca tgccggcgag cgccgggcgc accaccatgt gtcccaccga
    12481 gctgggctat gacggcgcca tcgagccctg cctgcgcctg ctgcccatag agacacgcct
    12541 gcaggcgtcc ggggtggccg agtggcgcct caggcccggc agctgggtca ggctggcgtt
    12601 gaacatggat gatccagagc agatggccag gacactggag aaactgtccg agatgcgcag
    12661 cgtgccgctg caggaggccc gcgcgctggg tttctggagc gacgaggcgg acggagccgg
    12721 cctgacgcct gatgccgacg gcatggtgca ggtgccggcg tggcgccacg ccatcgtcaa
    12781 cctgccgcat ccgctgctca agcagggact ggtgattctg gatacgccgg gcctgaacgc
    12841 cgtcggagcc gagcccgagc tgaccatcaa cctgattccg caagcgcatg cggtggtctt
    12901 catcctggcc gcggacacgg gcgtcacgcg ctctgacctg gccatctggc gcgaacacct
    12961 gggcgccgcc gaggatgcgg tggacgcgcg gctgatggtg ctcaacaaga tcgacacctt
    13021 gtgggatgag ttgaccccgc aggaacaggt gcaggcacag atcgagcgcc aatgcgccgg
    13081 cgccgcgcag ttgctgggcg tgcagccgga tcaggtcgtg gcggtgtcgg cgcaaaaggg
    13141 cttgctggcc aagatccgct gcgatgacga tctgttgcag gccagtggcc tgccgcagct
    13201 ggaggtgatc ctgggcacgg gcatcatggg ccggcgccag gccatcctgc gcgccacggt
    13261 gggcagcggc gtggccagcc tggaggccga gatacggcgc ctgctccatt tccgccggcg
    13321 cgaccttgac gagcagatgc tggagctgcg cagcctgcgc ggcaagaatg cttcggtgat
    13381 cgatgccatg cgtggccgca tcgaggtcga gcagcaggag ttcgaggcca gcacgaccaa
    13441 gatccaggcc gtgcgcgccg tgcacatgaa gatgctgcgc gatttgttcc agcggctggg
    13501 gccgcgcggg ctcagggtgc acctggcggc gctggccgag gcattgcagc acccgggcct
    13561 gaagctgggc gtgcgcaagg tgtatggcga ggcctttgac caggtgcgcg gcgccatcga
    13621 tgcggcccag gccgcgggcg gcgaaatcca ggccatgctt ggcggcactt tccgccagct
    13681 gaacaccgat ttcggtttct cgctgcaggt gccgcaggcg cccaccctgg cggcccatat
    13741 acgggagatc acggccatag agcggcgcca cctgcagtac ataggcctgg ccagcacgct
    13801 gcgcctggca cagccagagc atgggccgcg cctgctgcac gccctgactc aacgcctgcg
    13861 cacggtgttc gaggccgtgg ccaatgagct ggagctgtgg agcaactcgg ccagctccca
    13921 gctggaggcc cagatgcgtg agcgcaggca cagctttgcg cggcgcatcg aggccgtgaa
    13981 ccgcatccag gatgcagcca gcggcctgat ggagcgtatt gcggagatcg aggacgccga
    14041 gcgcaacctg gcggtgctgg agcagcgcct gctcgagctc accgcgcagc tggcgccgca
    14101 agtgcttgtc gatgaagctg ctgcggcccc ggacatggcc cagccacaga ccgagcccct
    14161 gcctttgacg cgcgtggcga cggcatgagc ggcgcaccgg tggagatcgc agcgcaggtc
    14221 gtgcgctggc aggcagagca tgggcgcaac cagctgccct ggcagaacac gcgcgacgcc
    14281 taccgcgtgt ggctgtccga aatcatgctg cagcagaccc aggtggtgac ggtgctggag
    14341 tactacgcgc gctttctggc gcgctttccc gatgtgcggc agctggcggc cgcgccgcag
    14401 gacgaggtgc tggcgctgtg gagtggcctg ggctactaca gccgcgcgcg caacctgcac
    14461 cgctgcgcgc agatcgtggt cgccgagcat ggcggcgagt ttccgcgcac ggttcatgag
    14521 ctggccgcgc tgccgggcat tggccgctcc accgccggcg ccattgccgc gttctgcttt
    14581 ggcgtgcgcg ccccgatact cgacgccaat gtgcggcgcg tgcttacccg cgtgctgggc
    14641 ttcggcgccg atctggccga ggtcaggaac gagcgcgcct tgtggagtct ggccgaggcg
    14701 ctgttgcccg ataccgatct ggccagcacc atgccgcgct acacccaggg gttgatggat
    14761 ctgggcgccg gcatctgcct gcccagaagc ccttgctgca tgctgtgccc gctgcagccg
    14821 gtctgcgtgg ccgggcgcgc tggcgatgcc gagcgctacc cggtgcgcac gcgcaagctc
    14881 aagcgccggg ccgaggcctg gtggctgctg ctgcgccagg acagggccgg ccgcctgtgg
    14941 ctggaaaggc gtgcgcccgt cggcatctgg gccgggttgt actgcccgcc ggtctatgcc
    15001 agccggcgtg atctggatga ggcgctggcg ccagcggttc gatcggaagt gcaggatctg
    15061 cccgccttta cccatgtgct cacgcaccgt gatctgcacc tgcacccggt gctggcacgc
    15121 tgcgacatgg ccgcgccagc tgggcagtgt tccgaggcat tggtggccga ttggttcacc
    15181 ccagagctgt ggcagggcct gggcctgccg gcgccggtgc gcaggctgct ggatgcgcta
    15241 taggcaccag gtttcgtaaa actgatagcg tttggcgctt gtattatggg taaatacagc
    15301 ggatttgccc ttcaaattct ttactgacaa gcgcaaaaca ctattgatta tgtagtgcca
    15361 tgccgaatcc catcgagctc gcggtgccgg cacagcgtgc tccagtgact ggcaaactgt
    15421 gcgctcagct gctccgccag gtacacggag cggtgctgtc cgcccgtgca gcctatggcc
    15481 acggtgacgt agctgcggtg gttttgcgcc agcgcatcca gccagtgctc cagaaactgg
    15541 ctgatgtcgg actgcatccg ctccacctcg ggttgctggc gcaggtattc gatcaccggc
    15601 gcgtccctgc cggtgagtgc gcgcagcgtg ggctcgtaat gcgggttggg cagcatgcgc
    15661 acgtcaaaca catagtccga gtcgaacggc acgccgtgct tgaaggcaaa ggactcgaac
    15721 accagcgtca gctggcccgg cttgaccgcc agcagttcct tgacgtaggt cagtagctgc
    15781 gccgggcgca gcacgctggt gtctatgacg taggactgct cgcgcagctc ggccagcagc
    15841 tggcgctcca gctctatggt ctgcaccagc gcaagccggg tttccggcag ctcgttctgt
    15901 gacaacgggt ggcgccggcg cgtctcggaa aagcgccgca ccagggtgcc ggtgctggcg
    15961 tccaggaaca gcgagcgcac ctgcacgccg gtctcgcgca gccggttgag ctgctgcggc
    16021 accagcggca gggcggtggc gctgcgcaca tccatggcca cggccacgcg ctggctgcgg
    16081 tgcgaatgct ccagcgcgac gaaggacgcc agcagctccg gcggcaggtt gtccacgcag
    16141 tagtagccgg agtcctccag cgcgcgcagc gccagggact tgcccgatcc ggacatgccg
    16201 gtgatgacga cgatttccag tgacatggtt caacccgtgg tggtggctgg gcgtgcgccg
    16261 gccagcattt cgcgggcatg cgccatggtg gtgggggact gctgctggcc gcccagcatg
    16321 cgggcgattt cggccacgcg ctcatcggcg tcggcgacgg ccacggcgct ggtcgtgccg
    16381 gcggcactgc gctgcttgga gaccaccaga tgctgatcgg cacaggccgc cacctggggc
    16441 aggtgggtca cggccagcac ctggcgcgcg cgcccgagct gctgcatcag ccggcccacg
    16501 gtctcggcca cggcgccgcc cacgcccgaa tcgacctcgt caaagatcag cgtgggcgcc
    16561 tcgcccagct cgctggtggt gaccgagatg gccagcgcaa tgcgcgaaag ctcgccgccc
    16621 gaggccacct tgcccacggg gcgcggcgtc atgccggggt ggctggcgac cagaaaggcg
    16681 acctgatcca tgccctgcgg gccgggctcg gccgccttgt ccacctgcac ctcgaagcgc
    16741 ccgccggtca tgcccaggcc ctgcatggcc tgggtgatgg cctgggccag ctgcggcgcg
    16801 gccttggcgc gcttttgtga cagggcgcgg gccgccttct ggtaagcggc ggcgtgggtt
    16861 tgctcggccg cctccagcgc cgccaggtcg gtggcgctgt cgagttggcg cagcgcctcc
    16921 ttccagcccg ccagcagcgc cggcagctcg ggcggctggc gcttgtagcg gcgtgccagc
    16981 tgcatccatt gcgccaggcg tgcatcgagc tgctccagca gctcggggtc gggctcggcg
    17041 tggcgcaggt aggcctgcag cgaatgcgcc acgtcgccgg cctgggcgat gcaggcgctc
    17101 agcacctcgt tgaaggcgcg aaatgatgcg tccacatgtt cgtagtcctg cagcagatgc
    17161 tgcgcctggg ccagcgcgct cagcgcgccg ccggcgtcgt cgccctgcag cgcgcccagg
    17221 gcgccctggg cgttgtccag cagggcctgg gcgttggaca ggcgtgcgtg ccgggcgttg
    17281 agctcgtccc attcatcgtc gctcggcgcc agcttgtcca cctcggtgat ctgccactgc
    17341 aggcgttcgc gctcctgctg cagcgtgtcc tgtgcggcgc gggcatggag cagggccttc
    17401 tgcgcctcgc gccaggtctg ccacagagcg gccaggggct gcgcctgcac gccggcatag
    17461 gcgtcgagca ggccgcgcac ggcgtccggc cgcgtcaggc tttgccaggc atgctggccg
    17521 tgtatgtcca gcagatgctc gcccagggcg cgcatctgcg tggccgtggc cggcaggccg
    17581 ttgatccagg cgcggctctt gccctgcagg tctatggtgc ggcgcagcag cagctcgtcg
    17641 tgcgggaagc cggcctcatc gagccagtcg gtcagtgctg cggggccgat gtcgaactcg
    17701 gcgctgatgt cggtgcgctc tgccccttcg cggatcacgc cgctgtcggc gcgtgcgccc
    17761 agcagcagct gcagtgcgtc aatcaggatg gacttgcccg cgccggtctc gccggtgagc
    17821 acggtgaagc ccgtttgcag atccagatcc agcgcctgca cgatgacgaa gtcgcgcagc
    17881 gcgatgcgcc tcaaggccat gttcaggagc ctccctcgtt ccagcgcagc ttcttgcgca
    17941 gggtggaaaa gtagctccag ccgcgcgggt gcaaaaagca cacgcgatgc gccgagcggc
    18001 gcaccagcac ccgatcgccg cgctgcagcg aggccagcga ctgcatgtcg aagttggcgc
    18061 tgatgccgcg cccgccggcc acctcgatgg cgatctcgga ggagtcggac agcacgatgg
    18121 ggcggttgga cagcgtgtgc ggcgcaatcg gcaccagcac ccagccgggg atggaggggt
    18181 gcagcatggg cccgcccgcc gacagcgagt aggccgtgga gcccgtgggc gaggcgacga
    18241 tcaggccatc ggcgcgctgg ttggagacga acaccccgtc cacctcgatg cgcagctcca
    18301 ccatgccggc ggtggagccg cggttgacga ccacgtcgtt gagcgccagc gcctcgaaaa
    18361 cgcattcgcc gccgcgcacc acgcgcgcct gcatcagcgg gcgcacgtcc tcctcgtact
    18421 cgccgtgcag tatggcggtc agcgaggtct cgtagctttg cagcggaatg tcggtgatga
    18481 agcccaggcg ccccttgttg atgcccacca gcggcgtgcc ataggcggcc agccgccggc
    18541 ccacgcccag catggtgcca tcaccaccca ccaccacgca caggtcgcaa tgcccgccaa
    18601 tggcgtccac atccagctgc gggtagccgg tgatgcccag ggtggctgcg gtttcggcct
    18661 ccagcgtggc ctcgccgccc tcctggatga tcaggcgggc aatgccctcg atgatctgcc
    18721 gcgagcttgc cggcggcgcg ccggcaacgg gctctcggta tttgcctatg actgcgacac
    18781 ggcggaagat gggcttcatg cggaaattac atcattaaaa tgagccgatg ctcgacgacc
    18841 gctccaagtt gttactcaag gcgctggtgg aacgctacat cgccgatggg cagccggtgg
    18901 gttccagaac cctgtcgcgg gcatcgggcc tggaattgtc acccgccacc atacgcaacg
    18961 tgatggccga cctggaggag ttgggcctga tcgccagccc ccacacctcc gccgggcgca
    19021 ttcccacggc gcgcggctac cgcctgttcg tcgacaccat gctcaccgtg cagcaggagc
    19081 agctgccggc cctgcagctc atgcccgaac agccgcaaaa ggtgatcgcc aacgcggcgc
    19141 agctgctgtc caacctgtcg cagttcgtcg gtgtggtcat ggcgccgcgc cgcgcctcgg
    19201 tgtttcggca tatcgagttt ctgcgcctgt ccgagcgccg cctgttggtg atcatcgtct
    19261 cgcccgatgg cgacgtgcaa aaccgggtga tcttcaccga ggccgactac accccgtcgc
    19321 aactggtgga ggcggccaac ttcctgaacg cccactacgc cggcctgacc atggagcagg
    19381 tgcgcgagcg cctgaagctt gaggtggaca agctgcgcgg cgagatcgcc acactgatgc
    19441 aggcggccgt gaacgtgggc tcgcaggcca tgtcggaatc gcaggacgag gtggtgttct
    19501 cgggcgagcg caacctgctg gcggtgagtg atttttctgg cgacatgggc catctgcggc
    19561 gcgccttcga cctgttcgag caaaaaaccc agatcctgcg cctgctcgat atctccagcc
    19621 gcgccgaggg tgtgcgcatc ttcatcggcg gcgaaagtca ggtcgtgcca tttgaagagc
    19681 tgtccgtggt cagcgcaccc tatgaggtcg atggccaggt ggtcggcacc ctgggcgtga
    19741 taggccccac gcgcatgccc tacgaccgca tgatccagat cgtggacatc acctccaaac
    19801 tggtctccaa cgctctgagc caccacaagt agccactaca atgcgcgatt gcgtcttgcg
    19861 caccctgggg cgttagctca gttggttaga gcagaggact cataa
//
LOCUS       DAOMTQ010000042        19806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_337_length_19806_cov_24.606096, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000042 DAOMTQ010000000
VERSION     DAOMTQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 19806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19806
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_337_length_19806_cov_24.606096"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK518_06095"
     CDS             complement(<1..270)
                     /locus_tag="PK518_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48519.1"
                     /translation="MTLQTMDRHARRRLLIAGAAGAMLGAAAPLFAQPGGGAAPERRA
                     RLALSGPFAAVSYPLMRIADSGALADVADKVEFSSWKTPDQLRALA"
     gene            complement(273..2072)
                     /locus_tag="PK518_06100"
     CDS             complement(273..2072)
                     /locus_tag="PK518_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRM48520.1"
                     /translation="MIRGLQKDSVVLMVDGMRLNSAQRAGAIASFLSLGLAERVEVVK
                     GPASVFYGTGALGGAINVRLPQARFEPGLHLKTRLGYDSASRGASGAALLNASQGDHA
                     LMLGGSLADYQDYRAPGGKVAYTGYESGAFIGQYCYRLGGGQQLRLSLQQQRDDDVWY
                     PGSIKPHALPVVGSVITHSPKQTRRLYELGYNRQGDGERPLNFDLRPYRQEMSRSIYG
                     QARLLGRDIVTTDVSFATDGLDARADWLLHPAHLLSFGINAWRMTADPDAWMARPPQF
                     VQYVPSQPFGDGRLQAVGLYLQDDIKLGAWSVLAGLRHDRVQGRAAMMNNGAVTSGLD
                     RSDGATSASLGVMHEVTPLLRPYANVSRAFRAADLRERYQSGLRNDGFYWAGSPQIRP
                     ETATQFELGMKGEDERFGYAVSAFRTRIANHITGMQLSGAAAMAACGAANAAACKQTV
                     NLGHATLTGLEASLRWRWRPGHWLDAGYSHVRGTNGDLHEPRYQMPADTLRLGCEGRI
                     LADWSMDATALLVRPQKRVATRFTRGQEDASAGYGVVDVGATWRYARGQSLRLAIKNL
                     GDKRYHEHLAEGLPGLEPYAPGRSLRIGWEGQF"
     gene            complement(2085..2375)
                     /locus_tag="PK518_06105"
     CDS             complement(2085..2375)
                     /locus_tag="PK518_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48521.1"
                     /translation="MQPLLRRLPGALVPLALGAGLHAGAVQAQPGMADRAGVASELPA
                     VSVTAKGYAASEVETPVASTVQTADDLARKQAQNLGEALRGEPGISVSLDGA"
     gene            2744..3739
                     /locus_tag="PK518_06110"
     CDS             2744..3739
                     /locus_tag="PK518_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48522.1"
                     /translation="MLRRHILSFATLACALGAATVQAQTAAPAAASAYPTKPIRMIVP
                     FPPGGGTDILARLVSAKLTEANKWTVVADNKPGAGGTIGIGEAVKAAPTGYDIVMGQK
                     DNLVIGPWLYKNLAWDPTKDLTAVAHVAYTPVVIATSVNSRFKTLADVVAAAKAAPGT
                     VTYGSPGNGTSIHLAGDLFEKAAGIKLSHVPYKGSNPALMDALAGNVELLVSSLPSAM
                     GQIKAGKLRPLAVTSAKRSSSLPDVPTVAESGFKGFDVSTWYGVFAPAGTPAAVVSTL
                     NAEVNKLLETADVKAAIHAQGAEPLAMTPEQFSTLLKAEYAQWKGIVEASGAKIE"
     gene            complement(3802..4242)
                     /locus_tag="PK518_06115"
     CDS             complement(3802..4242)
                     /locus_tag="PK518_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM48523.1"
                     /translation="MFKHILVPVDGSETSMKAVAKASALAKTFDSAVTALYVIDPYPF
                     TGVGADFAYGQAQYLSAATAEANAALDAAKAAINQAGVANVSTMVGEGHAVHDGIQRA
                     IESTGADLIVIGSHGRRGLEKLVLGSVTQRVLGVARVPVLVVRE"
     gene            complement(4336..5088)
                     /locus_tag="PK518_06120"
     CDS             complement(4336..5088)
                     /locus_tag="PK518_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HRM48524.1"
                     /translation="MAHVSFSPPYTPSSQMTQQLRDRGYAVLQPHDTAVWLGCDLADL
                     QALASDWADLPPDDFLKDGGRYRRRRHSCFIVDGSAVSQTPHRAHWQPVEYNALHGGM
                     QRWFAPMRDATVQAPVWGRLMRALGGLAQQALATRSETWFIEAHQFRIDTEGGIGRPT
                     PEGAHRDGVDLVAVFLVDREGVKGGETRIFEATGPNGQRFTLSEPWSVMLLDDARMIH
                     ETTPIQPDGEHGWRDTLVLTCRRDGFLEDAGQ"
     gene            5393..6400
                     /locus_tag="PK518_06125"
     CDS             5393..6400
                     /locus_tag="PK518_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HRM48525.1"
                     /translation="MNIVILDDYQDAVRKLHCAVRLDPYSAKVYTNNVKGLGQLSVRL
                     RDADIIVLIRDRTQINRQLVDKLPRLKLIAQTGRVGSHVDIAACTERGIAVAEGVSSP
                     VAPAELTWALIMAAMRRLPQYISNLKHGAWQQSGLRAASMPANFGLGSILRGKTLGIW
                     GYGRIGQIVAGYGRAFGMNVRVWGREASRAQALSDGLQVASTRAEFFSQCDVVSLHLR
                     LTDETRGMVTLEDLSCMKPTALFVNTSRAELIETDALLAALNRGRPGMAAVDVFESEP
                     ILQGHALLRLENCICTPHIGYVEQDSYELYFGAAFENVVNYIKGTPTNIVNPGALQVR
                     R"
     gene            complement(6507..7634)
                     /locus_tag="PK518_06130"
     CDS             complement(6507..7634)
                     /locus_tag="PK518_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM48526.1"
                     /translation="MQLKLKLTVAAAIAAIAGVAAAQEVVRIGHVAPISGAQAHYGKD
                     NENGSRMAIEELNAQGVEIGGKKVKFELLAEDDAADPKQGAAVAQKLCDSKAAGVVGH
                     LNSGTTIPASKVYNDCGIPHITGAATNPNLTKPGYKTTFRIIANDNALGAGLASYAAD
                     TLKLKTVAVVDDRTAYGQGLANVFKRVAAEKGIKVVDEQFTTDKATDFMAILTAIKSK
                     NPDAVFYGGMDPQAGPMLRQMEQLGMGNVKFFGGDGVCTTEIAKLAGGAKTLGNVVCA
                     EGGSSLAKMPGGMAWKAKYDAKFPGQYQIYSPYTYDATMLMVDAMKRAGSSDPKVYIP
                     ELLKSNYKGVTATIAFEPNGEIKNPAITLYVYKDGKKTPLN"
     gene            complement(7774..8196)
                     /locus_tag="PK518_06135"
     CDS             complement(7774..8196)
                     /locus_tag="PK518_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48527.1"
                     /translation="MGMPPKTPPRFVPTLTDVVHLPDTARFAGQIIEATAASEAPQPV
                     AAAPEGLQNLAPGPVPDADTAAGSSLPEGLEEYVVHRVMQRVDLVLDQRLREAIALVV
                     QEQTRSMIPRLREEVESVVRHAVYEAVADELAENGGRR"
     gene            complement(8197..8634)
                     /locus_tag="PK518_06140"
     CDS             complement(8197..8634)
                     /locus_tag="PK518_06140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRM48528.1"
                     /translation="MTEVAFHFNAPDKLAYVCRLARKATRHDMRVLITASRQDLQRLD
                     RLLWALGPSDFVAHCMDDADEEMRQASAVLLATDPLSCDPRDLLVNLGDAVPQGFEQF
                     ARLVEVVSQSDSADRSRARERWRHYAAQGYAIVRHDLVLRGGD"
     gene            complement(8662..10152)
                     /locus_tag="PK518_06145"
     CDS             complement(8662..10152)
                     /locus_tag="PK518_06145"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRM48529.1"
                     /translation="MNFELKTLSLSAAVSHKCDLLILLVPEGFKPQGDALSVLVATAL
                     KQGDLELKPGKHLALYAPAEMAARRLVLLGCGKGDPHAVRQAVQALGATLKTRAIKRA
                     ALVFATDCEAGSVGAAVRTMAEAGYVYTATKSKAEMPALSRVTVGVSDAGAVQPEFAI
                     ATALAVGVEYAREWANRPANHATPTLIGEAAKALGKHAGIQCKLHGPAQVAKLGMGAF
                     LAVAQGSDQPLRLIELRYDGAARSQAPVVLVGKGITFDTGGISLKPAAEMDEMKFDMG
                     GAASVLGVFRALAELKPAINVVGLIPACENMPDGKAIKPGDVVTSMSGQTIEILNTDA
                     EGRLVLCDALTYAARFKPAALIDIATLTGACVVALGGLRSGLFASSDELAARLAAAGD
                     AALDPCWRMPLDDDYAEGLKSNFADVANVAGRAGGSITAAKFLQRFVGDIPWAHLDIA
                     GTAWKGGTAKGATGRPVGLLLHYLLGMAGAPTPRRKTAAARRARAD"
     gene            10176..11264
                     /gene="lptF"
                     /locus_tag="PK518_06150"
     CDS             10176..11264
                     /gene="lptF"
                     /locus_tag="PK518_06150"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRM48530.1"
                     /translation="MLFDSSIRKELARSFGATLVVLITVVMTMMLIRTLGQASRGSVA
                     PADVMLVMGFTVLGQLPTILSLSLFITVVGTLSRMYRDSEMVIWFSAGRGLMSLLAPL
                     LRFSWPVLLVIAALSLVVWPWANRQIQELKTQYEQRSDVDRIAPGEFQESANGARVFF
                     IDKDTPDANAANNVFIATREQRGETVTSAQSARLEIRSGERIVLLRNGQRLETAPDKP
                     GLRISEFAEYGTLIGGGPQGGTDAGAIKTRDTWDLAQDPQSQAELGWRLGSTLAALNF
                     IVLGLAVAIVNPRAHRSTSLVLALLAFMVYYNLMTVGQAWVGAGRVGILPFLLALHGG
                     TLALGLLLVTARHNHWSLRALLPRGSAA"
     gene            11261..12367
                     /gene="lptG"
                     /locus_tag="PK518_06155"
     CDS             11261..12367
                     /gene="lptG"
                     /locus_tag="PK518_06155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRM48531.1"
                     /translation="MRTIRRFIYREVVVSVAFVTLAFLALFFFFDLVDELRWVGRLGM
                     GYQLTHALLFVALGLPQHLYELLPITVLIGTIFVMARLAQSSEFTIMRTSGLGPWRAL
                     STLLLLGCAFVALTFAVGDYLAPISERAAALVKARGQGQISTGATGAWLKEREGDHSF
                     AVNVGAIDGNGRMRGVRVFEFDERGRIASTTTAEQAEFADNGAGWNLQQVQRTVFEQQ
                     TDGDMTVTKLQEPRLLWQTRISPDMVAASLLKPDKMATIELFHYIRHLEANGQAAQRY
                     EIEFWRKVFYPLSCLVMVVLSLPFAYLHFRSGGIAGYVFGGVMAGISFFLLNNVFGFA
                     GNLQNWSPWLTAAAPGLIYSVLSLAAFGWLVLRR"
     gene            12402..12782
                     /locus_tag="PK518_06160"
     CDS             12402..12782
                     /locus_tag="PK518_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRM48532.1"
                     /translation="MQQSQLAIILLAHGSRDPLWRRPIEAVAARIQTDQPRLLVRCAY
                     LELCAPDLAGAVAELATLGATQITIASLFLGAGRHVREDLPRMVQDLAHNFGHLQLRL
                     QPPIGEDARMTALMADIASQSIAS"
     gene            12849..13790
                     /locus_tag="PK518_06165"
     CDS             12849..13790
                     /locus_tag="PK518_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HRM48533.1"
                     /translation="MNLHQFRFVQEAARRNLNLTEAAKALHTSQPGVSKAIIELEDEL
                     GVDIFARHGKRLKRVTEPGQHVLKSIEIIMREVGNLKRIGEQYSAQDSGTLAIATTHT
                     QARYVLPVPVARLREAYPKVNISLHQATPHEVARMVIDEVVEIGMATESLADYPDLVT
                     LPCYEWQHVLVLPPAHPLAGKERIGLEDIAHEGLITYHPSFTGRGKIDQAFATRKLQP
                     RIVLEAIDSDVIKTYVRLGLGIGIVAEMAVRDDPVGDLVVRPVGHLFGQSVARVAFKR
                     GAYLRNFVYRFAELLSDRLSRELIARAMTGHVNDYEL"
     gene            13768..>13970
                     /locus_tag="PK518_06170"
     CDS             13768..>13970
                     /locus_tag="PK518_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HRM48534.1"
                     /translation="MSTTTNSEQTVKQLTPPAFPSRLPNVGTTIFTVMSALAAEHQAV
                     NLGQGFPDFACDPALTDAVTRAM"
     assembly_gap    13971..14070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14071..14559
                     /locus_tag="PK518_06175"
     CDS             <14071..14559
                     /locus_tag="PK518_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM48535.1"
                     /translation="LAARAFIVSSFGKTFHVTGWKVGTVAAPAAMTAEFRKVHQFNVF
                     TVNTPMQYGLAEYLQDPTPYLQLPAFYQAKRDLFRQGLAGSRLKLLPSTGSYFQCVDI
                     SAVSDLSEADFCQWLTREIGVAAIPLSAFYGDGFDQRVVRFCFAKKDETLRTAIERLR
                     RL"
     gene            14575..15702
                     /locus_tag="PK518_06180"
     CDS             14575..15702
                     /locus_tag="PK518_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM48536.1"
                     /translation="MRAILLLIAVMISYGSLYPFHFAANHHWLAEAAGLFSMPTLRMG
                     RGDLVGNLLLFVPYGLVAALHVDTRRPVRASGTLALLLGLGLLLALALQLAQIWVPSR
                     VPELNDVIANGVGMVLGTLAGWVARSLFPNAAMQLPRALAPAMLMLLWIGYQWAPLVP
                     TLDLQNILNALKPLVHSPQVDAVRALHTTLAWLAFFKLWELAAGKTLSTPVMAMAALG
                     IVGAKLFIVGASISATNALALGLALVCLPWRQHAMAVPVLSIAMFISLAASGLAPYEP
                     LGQAQAFHWIPFTGMLQGSMGTNLLNLAEKSFFYGALIMLIGAPLSHPLAAAGMVAFF
                     LGLIEAAQLFLPGRVAESTDPVLALILGLVIRLLSVRGKPA"
     gene            complement(16074..16277)
                     /locus_tag="PK518_06185"
     CDS             complement(16074..16277)
                     /locus_tag="PK518_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48537.1"
                     /translation="MVDKFHYQVFLGAVANAGAYDAAHILNQRGFVFALFVVPKDQLK
                     DGSSSFRVEVAKVGQMVWGVSPP"
     gene            16194..16583
                     /locus_tag="PK518_06190"
     CDS             16194..16583
                     /locus_tag="PK518_06190"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48538.1"
                     /translation="MVEDVRRVIRASIGNRAKEDLVVEFINHTNLDELGDKATVIDAF
                     FQYAQAEQQREAQALIDEEKLNVEAAKRYITNSLKREFASDSGTELNAVLPKMSPLNP
                     QYLTKKQTVFQKIAAFVEKFKGVGGQV"
     gene            complement(16708..17010)
                     /locus_tag="PK518_06195"
     CDS             complement(16708..17010)
                     /locus_tag="PK518_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRM48539.1"
                     /translation="MTKLALFVRLEAKPGQEAALADFLASALPLANAESGTTAWFALK
                     FGPSTFGVFDAFADEAGRQAHLNGQIAAALMANAATLLSSPPNIEKVELLAAKLPA"
     gene            17077..18090
                     /locus_tag="PK518_06200"
     CDS             17077..18090
                     /locus_tag="PK518_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM48540.1"
                     /translation="MTDISIGNNDNIDIIVAMKLHLLVCDGVFDLGLAALTDTVGLAN
                     AMAGSLPQAPAHIELTLVGVRRRIRTAQGLTVPVVTAHGVPEPDVVLVPAFGDKMPDT
                     LSARLTRPDVPDAVAALQHWSTAGAHLGAACSGSFLLAESGLLDGHRATTSWWLGPMF
                     RQRYPNVTLDESRMIVNSTRFTTAGAALAHVDLALRIIRGRSPALAALVARYLLVETR
                     SSQAEFVIPDHLAHADPMVERFECWARRRLAQGFSLAEAASAAGTSERTLARRLQSVL
                     GKTPLSYFQDLRVEHAVHLLRTGNASVDQVAAQVGYSDGVTLRALLRRKLGRGVRELR
                     RGG"
     gene            18337..19401
                     /locus_tag="PK518_06205"
     CDS             18337..19401
                     /locus_tag="PK518_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRM48541.1"
                     /translation="MLFNPLQVGSLTLPNRILLAPLTRTRADAGHMPNALMAEYYSQR
                     ATGGLLISECTMVAPGTSAFVNEPGIYNDAQIAAWRQVTDAVHAKGGRIFMQIWHAGR
                     AAYPGAADGAPIVSSSATAIEGEIHTPQGKVPHAVPRPLTAEEIPGIVAAFAQGARNA
                     IAAGFDGVEVHGANGYLIDQFLRDTPNQRTDAYGGSLENRARLLFEVLTAVTQAIGSE
                     RVGLRLSPLNSYNSMKDSDPLALIGFLADKLNAFKLAYLHVMRADFFGVQKADVMPVA
                     REKYKGVLVGNMGYSADEAEAAIAAGKLDAVAFGTAFLANPDLPTRISAKAPLNAPDT
                     NTFYSGGAKGYTDYPTLQVA"
BASE COUNT         3589 a         6485 c         6004 g         3628 t
ORIGIN      
        1 ggccagagcg cgcagctgat ccggcgtctt ccagctgctg aactccacct tgtcggccac
       61 atcggccagc gcgccgctgt cagcgatgcg catcaggggg taggacacgg cggcgaacgg
      121 gccggacagc gctagccttg cgcgccgctc cggggctgcg ccgcccccgg gttgcgcaaa
      181 gagcggcgcc gccgcgccca gcatggcacc cgctgcgcca gcgatgagca ggcggcggcg
      241 tgcgtgacga tccatggtct gcagggtcat ggtcagaatt gtccttccca gccaatgcgc
      301 aggctgcgcc cgggggcata gggctccaga cccggcaaac cctcggccag atgttcgtgg
      361 tagcgcttgt cgcccaggtt cttgatggcc aggcgcagcg actggccgcg tgcataacgc
      421 caggtggcgc cgacatcgac cacgccgtag ccggcgctgg catcttcctg gccgcgcgtg
      481 aagcgggtgg caacgcgctt ttgcggccgc accagcagcg ccgtcgcgtc catcgaccag
      541 tccgccagaa tgcgcccttc gcagcccagg cgcagggtgt cggcgggcat ctggtagcgc
      601 ggctcgtgca ggtcaccatt ggtgccgcgc acatgcgagt agccggcatc cagccaatgg
      661 ccaggacgcc agcgccagcg cagcgaggcc tccagcccgg tcaaggtcgc atgccccagg
      721 ttgacggtct gcttgcaggc cgcggcattg gcggcgccac aggcggccat ggcggctgcg
      781 ccactgagct gcatgccggt gatgtggttg gctatgcgcg tgcggaacgc cgacacggcg
      841 tagccgaacc gctcatcctc gcccttcatg cccagctcga actgggtcgc cgtctcaggc
      901 ctgatctgcg ggctgccggc ccagtagaaa ccgtcattgc gcagcccgga ctggtagcgc
      961 tcgcgcagat cggcggcgcg gaaggcgcgc gagacattgg cgtaggggcg cagcagcggc
     1021 gtcacctcgt gcatcacgcc caggctggcc gaggtggcac cgtccgaccg atccagcccc
     1081 gaagtcacgg cgccgttgtt catcatcgcc gcacgcccct gcacgcggtc gtggcgcagg
     1141 ccggccagca cgctccaggc gcccagcttg atgtcgtcct gcaggtacag gcccacggcc
     1201 tgcaggcggc catcgccaaa tggctgcgac ggcacgtact gcacgaactg cggcgggcgc
     1261 gccatccagg catcggggtc ggccgtcatg cgccaggcgt tgatgccgaa cgacagcagg
     1321 tgcgccgggt gcagcaacca gtcggcgcgc gcgtccaggc catcggtggc aaagctcacg
     1381 tcggtggtga cgatgtcgcg ccccagcagc ctggcctggc catagatgct gcggctcatc
     1441 tcctggcggt acgggcgcag gtcaaaattg agcggccgct cgccatcgcc ctggcggttg
     1501 tagcccagct cgtagaggcg gcgcgtttgc ttgggcgaat gggtgatgac gctacccacc
     1561 accggcagcg catgcggctt gatggagccg ggataccaca catcgtcgtc gcgctgctgc
     1621 tgcagcgaca ggcgcagctg ctggccgccg cccaggcggt agcagtactg gccgatgaag
     1681 gcgcccgatt catagccggt gtaggccacc ttgcccccgg gggcgcggta gtcctggtag
     1741 tcggcgagtg acccgcccag catcagcgcg tggtcgccct ggctggcatt gagcagcgcc
     1801 gcgccgctgg cgccccggct ggccgagtca tagcccaggc gggtcttgag gtgcaacccg
     1861 ggctcgaagc gcgcctgcgg caggcgtacg ttgatggcac cgccaagcgc ccccgtgcca
     1921 tagaacaccg aggccgggcc cttgaccacc tcgacccgtt ccgccaggcc cagcgacaga
     1981 aacgaggcga tcgctcccgc ccgctgcgcc gaattcaggc gcatgccgtc caccatcagc
     2041 accacgctgt ccttctgcaa gccacgaatc atcgggttct gcccctacgc gccatcgagc
     2101 gacaccgata tccccggctc tccgcgcagc gcctcgccca ggttctgtgc ctgcttgcgc
     2161 gccagatcgt cggccgtctg cacggtggac gccaccggcg tctccacctc ggatgcggca
     2221 taacccttgg cggtgacgct gacggcgggc aattcagacg ccacgccagc ccggtcagcc
     2281 atacccggct gggcctgcac ggcacccgca tgcaggccag cgcccaatgc cagcggcacc
     2341 agggcgcctg gcaggcggcg cagaagcggc tgcatacggc cgcctggcgc cggacagcgc
     2401 ccgctgggcg aaagcggcct gtgatcggca tgcccgtgcg gctgcacctt ccaacctgct
     2461 tcggtggagg caaacgagat atctgctgtc atggagtggc tgatccgagt tggcatccgc
     2521 ccatgataac gataacgatt cgtattaacg atgagccaga tcaaacccgg tacccaaccc
     2581 gcgccacatg gcctgcgggc cacgctgatg caggcatcgc gtttggcgat ggcacgcacg
     2641 cgcatgggca gggctggcat cacggcgact gggccatcat gctgcccgtt atgcagccac
     2701 cggctgtggc tggcttttct cggcttccct ggagacttcc ttgatgcttc gcagacatat
     2761 cctctcgttt gccaccctgg cctgcgccct tggcgctgcc accgtccagg cccagaccgc
     2821 tgcccccgca gctgccagcg cctaccccac caagcccatc cgcatgatcg tgcccttccc
     2881 gcccggtggc ggtaccgaca tcctggcgcg tctggtgtcc gccaagctca ccgaagccaa
     2941 caaatggacc gtggtggccg acaacaagcc tggcgccggc ggcaccatag gcattggcga
     3001 ggccgtcaag gccgcgccca cgggctacga catcgtcatg ggccagaagg acaacctggt
     3061 cataggcccc tggctgtaca agaacctggc ctgggatccc accaaagacc tgacggccgt
     3121 ggcccatgtg gcctacacgc cggtggtcat agccaccagc gtcaactcca ggttcaagac
     3181 cctggccgac gtggtggccg ccgccaaggc cgcgcccggc accgtcacct atggctcgcc
     3241 gggcaatggc acctccatcc accttgccgg cgacctgttc gagaaggccg caggcatcaa
     3301 gctgagccat gtgccctaca agggctcgaa ccccgcgctg atggacgcgc tggcgggcaa
     3361 tgtggagctg ctggtctcgt ccctgccctc ggccatgggc cagatcaagg ccggcaagct
     3421 gcgcccgcta gccgtcacct cggccaagcg cagttcctcg ctgccggatg tacccacggt
     3481 ggccgaatcg ggtttcaagg gctttgatgt cagcacctgg tatggcgtct tcgctccggc
     3541 cggcacgccc gcagccgtgg tcagcaccct caacgccgag gtcaacaagc ttctggagac
     3601 agccgatgtg aaagccgcca tccacgccca gggagccgaa cccctggcca tgacaccgga
     3661 gcagttctct accctgctca aggccgagta cgcgcagtgg aagggcatcg tcgaagcctc
     3721 cggcgcgaag atcgaatagg cggcccggcc tgcccaacaa aaacggctcc cgagggagcc
     3781 gtttttgttg ggaagaaaac cttactcgcg caccaccagc acgggtacgc gggccacgcc
     3841 cagcacgcgc tgggtgacgc tgcccagcac cagcttttcc aggccacggc ggccatgcga
     3901 tccgatgacg atgaggtcag cgcccgtgct ctcgatggcg cgctggatgc cgtcgtgcac
     3961 ggcatgcccc tcgccaacca tggtgctcac attcgcgacc ccggcctgat tgatggcggc
     4021 cttggccgca tccagcgcgg cattcgcctc ggccgtggct gcactcagat attgcgcctg
     4081 gccgtaggca aaatcggcgc ccacgcccgt gaacgggtag gggtcgatca catacagcgc
     4141 cgtcaccgca ctgtcaaagg tcttggccag ggccgatgcc ttggcgacgg ctttcatgga
     4201 tgtttccgag ccgtcgacgg gaaccagaat gtgcttgaac atggaacctc ctcattgaaa
     4261 tcgggatagg gagatgatcc ctgagctgtt gccagtgtga cgccatgcgg cgccttggtg
     4321 cttgatgcgc atcaattatt gccctgcatc ctccaggaag ccatcccggc ggcaggtcag
     4381 taccagcgtg tcacgccagc catgctcacc gtcgggctgg atgggcgtgg tttcgtgaat
     4441 catgcgcgca tcgtcgagca gcatcaccga ccagggttcg ctcagtgtga agcgttgccc
     4501 attggggcct gtggcctcga agatgcgcgt ctcaccaccc ttgacccctt cgcgatctac
     4561 caggaatacc gcaaccagat ccacgccatc gcggtgcgcc ccctcgggcg tggggcggcc
     4621 aatgccaccc tcggtatcaa tgcggaactg gtgtgcctcg atgaaccacg tctcggaccg
     4681 ggtcgccaag gcctgctgcg ccaagccacc cagggcccgc atcagacggc cccaaaccgg
     4741 cgcctgtacg gtggcatccc gcatgggggc aaaccagcgc tgcatgccgc catgcaaggc
     4801 gttgtactcc acgggttgcc agtgggcgcg gtggggtgtc tggcttaccg cgctcccatc
     4861 cacaatgaag cacgaatggc gccggcggcg gtaacgtccg ccgtctttca gaaagtcgtc
     4921 cgggggcaaa tcggcccagt ccgacgcaag cgcctgcaga tcagccagat cacagcccag
     4981 ccagactgcg gtgtcatgcg gctgcagcac ggcataaccg cggtcacgca actgttgcgt
     5041 catctggctc gatggggtgt agggaggaga aaagctgaca tgtgccacgg cgcaagctta
     5101 acgacttggc gccttttgtt ctggcccgtg tgcacaaaga tacttcggga tcaatgaccg
     5161 ccgaacccga cacatcacac cgaggcgttg cctgacaacg tattcacagg tttgtcatct
     5221 tgtgtggtcg ctggactgga tttcagcacc ctggcgcaca tgggcccggt catgaacgac
     5281 aatcgttccc tggctcatca gtttcgccaa gccattgtga ctttttgccc gtcaggccgc
     5341 aggagcctca ggtgcttcgc ccccggccgc ccgacggaat gcacaccaca agatgaacat
     5401 cgtaatactc gacgactacc aggacgcagt gcgcaagctt cactgcgcgg tgcggctcga
     5461 cccgtactcg gccaaggtct acaccaacaa cgtcaaaggc ctgggacaac tgtcggtgcg
     5521 cctgcgggac gcagatatca ttgtgctgat ccgcgaccgc acgcagatca accgccaact
     5581 ggtggacaaa ctgccgcggc tcaagctgat tgcacaaacc gggcgcgtag gctcgcatgt
     5641 cgacatcgcg gcctgcaccg aacgcggcat tgccgtggcc gagggcgtga gctcacccgt
     5701 ggcccccgca gagctgacct gggcgctcat catggccgcc atgcgccgcc tgcctcagta
     5761 catctccaac ctcaagcacg gcgcatggca acagtccggt ctgcgggccg catccatgcc
     5821 cgccaatttc gggctgggca gcatattgcg tggcaagacc ctgggcatct ggggttatgg
     5881 gcgcatagga caaatcgtcg ccggatatgg ccgcgcattc ggcatgaacg tgcgcgtctg
     5941 gggacgagaa gcatcacgcg cccaggcctt gagcgacggg ctgcaggtgg ccagcacaag
     6001 ggctgagttt ttctcgcagt gcgacgtggt gtcgctgcac ctgcggctga ccgacgagac
     6061 ccgcggcatg gtcacgctgg aggacttgtc gtgcatgaag cctactgcgt tgttcgtgaa
     6121 cacctcgcgc gccgaactca tcgaaaccga cgcactgctg gccgcactca atcgcgggcg
     6181 cccaggcatg gctgcggtgg atgttttcga aagcgagccc attctccagg gtcatgcgct
     6241 gctgcggctg gaaaactgca tctgcacacc ccatatcggg tatgtggagc aagacagcta
     6301 cgagctttat tttggcgccg cgttcgagaa cgtggtgaac tacatcaagg gcacgcccac
     6361 caacatcgtg aaccctgggg cattgcaggt gcgccgctga aatcgcacca atctggcttt
     6421 ttgccgaaaa aaaggggctt catcgaagcc cctttttgtt gaccacaggc ggcactggaa
     6481 ggtaccgcca tgcatcatca atcgaatcag ttcaacggcg tcttcttgcc atccttgtag
     6541 acgtacaagg tgattgccgg gttcttgatt tcgccattcg gctcaaaggc gatggtcgcg
     6601 gtcacgccct tgtagttgga cttgagcagc tcggggatgt acaccttggg gtcggaggat
     6661 ccggcacgct tcatggcgtc caccatcagc atggtggcgt cgtaggtgta ggggctgtag
     6721 atctggtact ggccagggaa cttggcatcg tacttcgcct tccaggccat gccgccgggc
     6781 attttggcca gagagctgcc accctcggca cacaccacgt tgcccagcgt cttggcgccg
     6841 ccggcaagct tggcgatttc ggtggtgcac acaccgtcac caccgaagaa cttcacgttc
     6901 cccatgccca gctgctccat ctgacgcagc atggggccag cctggggatc catgccaccg
     6961 tagaagacgg catcggggtt cttggacttg atggcggtca ggatggccat gaaatccgtg
     7021 gccttgtcgg tcgtgaactg ctcatcgacg accttgatgc ccttctcggc agccacacgc
     7081 ttgaacacat tcgccaaacc ctggccataa gccgtgcgat cgtccaccac ggccacggtc
     7141 ttgagcttca gcgtatcggc agcgtacgag gccagaccgg cacccagggc attgtcattg
     7201 gcaatgatgc ggaacgtggt cttgtagccg ggcttggtca gattggggtt ggtggcagcg
     7261 cccgtgatat gcggaatgcc gcagtcgtta tagaccttgg aagccggaat cgtggtgccc
     7321 gaattgaggt gaccgaccac gcccgcagcc ttggagtcgc acagtttctg tgcaacggcc
     7381 gcgccttgtt tggggtcggc cgcatcgtct tctgccagca gctcgaactt gaccttcttg
     7441 ccgccgatct ccactccttg agcattcagc tcttcaatgg ccatgcggga gccgttctcg
     7501 ttgtccttac catagtgtgc ctgggcgccc gagatcgggg ccacgtgacc aatgcgtacc
     7561 acttcttgag cggcggccac gccggcaatg gctgcgatcg cagctgctac ggtcagtttc
     7621 aacttcagtt gcatatgggt ctccaaaaaa agaaaagcgc tggaaataag atgttgtcca
     7681 acgaccggaa acatatcgca atttttgggc ccctccacta gggaacacga gaaagtgccc
     7741 aagattcaca ctgggaaata cccggtcaac tgatcaacgc cttccaccgt tttccgccag
     7801 ttcgtcggca accgcctcat atacggcatg gcgcacaacc gactccacct cttcgcgcag
     7861 ccgtgggatc atggagcggg tctgctcttg caccaccagc gcaatggcct cgcgcaggcg
     7921 ctgatcgagc accagatcga cgcgctgcat gacgcggtgc accacgtatt cctccaggcc
     7981 ttcgggcaga ctgctgcccg cagcggtatc ggcatcaggc accggcccgg gcgccagatt
     8041 ctgcaaaccc tctggcgcag ctgcgaccgg ttgcggcgct tcggatgctg ccgttgcctc
     8101 aatgatctgg cccgcaaacc gtgccgtgtc gggcaaatgc accacatcgg tgagagtggg
     8161 cacaaagcgt ggcggcgttt tcggcggcat ccccattcag tccccccctc gcagcaccag
     8221 gtcatgccga acaatcgcat agccttgggc ggcgtagtgg cgccagcgct cgcgcgcgcg
     8281 gctgcggtcg gctgaatctg actggctgac cacctccacc agcctggcaa attgctcgaa
     8341 gccctgtggc accgcatcac ccagattcac cagcaagtcg cgcggatcac atgacagcgg
     8401 gtcagttgcc agcagcaccg ctgaggcttg ccgcatctcc tcgtcagcat cgtccatgca
     8461 gtgggcgacg aagtccgacg gccccagtgc ccacagcaag cgatcgaggc gctgcagatc
     8521 ctgccggctg gccgtgatca gcacgcgcat gtcgtggcga gtcgccttgc gcgccaggcg
     8581 acagacatag gccagcttgt caggggcatt gaaatgaaaa gcgacttcgg tcatgcgccc
     8641 aacaacttgc tgatgccgcc ttcaatccgc acgtgcgcgc ctggctgcag ccgtcttgcg
     8701 cctgggggtt ggcgcgcccg ccatgcccag caggtagtgc agcagcaacc cgacggggcg
     8761 ccccgtggca cccttggcgg tgccgccttt ccaggcggtt ccggcgatgt ccaggtgcgc
     8821 ccacggaatg tcgcccacaa aacgctgcag gaacttggcg gccgtgatgg agccaccagc
     8881 tcggcccgcc acgttggcga catcggcaaa attgcttttc aggccctcgg catagtcgtc
     8941 atcgagcggc atgcgccagc aggggtcaag cgccgcatcg ccggctgccg ccaggcgtgc
     9001 ggccagctcg tcgctggatg caaacaagcc gctgcgcagc cctcccagcg ccacgacgca
     9061 ggcgcccgtg agcgtggcaa tgtcgatcag ggctgcaggc ttgaaacgcg cagcgtaggt
     9121 gagtgcgtcg cacagcacca ggcgcccctc tgcatcggtg ttcaggattt caatggtctg
     9181 gccactcatg ctggtcacca cgtcaccagg cttgatggcc ttgccgtcag gcatgttctc
     9241 gcacgctggg atcaagccaa ccacattgat ggcgggcttg agctcggcca aggcccggaa
     9301 cacgcccaac acactggccg cgcctcccat gtcgaacttc atctcgtcca tctcggcggc
     9361 aggcttgagc gaaatgccgc cggtatcgaa ggtgatgccc ttgccgacca gcaccaccgg
     9421 cgcctgcgaa cgcgccgcgc cgtcatagcg caactcgatc aagcgcagcg gttgatccga
     9481 accctgcgcc acggccagaa acgcccccat ccccagcttg gccacctgcg ccgggccatg
     9541 cagcttgcac tgtatgccgg catgcttgcc cagcgccttg gccgcctccc cgatcaacgt
     9601 cggcgtggcg tggttggcgg gacgattcgc ccattcgcgc gcatattcaa cacccacggc
     9661 aagtgccgtt gcgattgcga actccggctg cacggcaccg gcgtcggaca cacccaccgt
     9721 gacgcgggac agtgcaggca tctccgcctt cgatttggtg gccgtgtaga catagccggc
     9781 ctccgccatg gtgcgtaccg cggcgccaac ggaacccgcc tcacaatccg tggcaaacac
     9841 cagcgctgcg cgcttgatgg cgcgcgtttt cagcgtagcc ccaagcgcct gcaccgcctg
     9901 gcgcaccgca tgggggtcac ccttgccgca gcccagcagc accagcctgc gcgccgccat
     9961 ctcggccggc gcatacagcg cgagatgctt gcccggcttc agctccaggt ctccctgctt
    10021 gagcgcggtg gcaaccagga cggacagggc atcgccctgc ggcttgaacc cttcgggcac
    10081 cagcaatatc agcaaatcac atttgtgcga aacagcggcg gacagtgaaa gcgtcttgag
    10141 ttcaaagttc ataatcggcg ttttccttcg agccaatgtt attcgattca tccatacgca
    10201 aggagctggc ccgcagcttt ggcgcgaccc tggtggtgct gatcaccgtg gtcatgacca
    10261 tgatgctgat ccgcacgctg ggacaggcct cgcgcggcag cgtggcaccg gccgacgtga
    10321 tgctggtgat ggggttcacg gtgctggggc agctgcccac cattctcagc ctgagcctgt
    10381 tcatcaccgt ggtcggcacg ctgtcgcgca tgtaccgcga cagcgaaatg gtgatctggt
    10441 tctcggccgg gcgtggcctg atgagcctgc tggcgccgct gctgcgtttt tcctggcccg
    10501 tgctgctggt gattgcagcg ctgtcgcttg tggtctggcc atgggccaac cggcagatcc
    10561 aggagctcaa gacccaatac gaacagcgca gcgatgtgga ccgcattgca ccgggagagt
    10621 ttcaagagtc tgccaacggc gcacgcgtgt tcttcatcga caaggacaca cccgacgcga
    10681 atgccgccaa caacgtgttc attgccacgc gcgagcagcg gggcgagacc gtgacatccg
    10741 cccaaagcgc gcgcctggag atacgcagtg gcgagcgcat cgtgttgctg cgcaacggcc
    10801 aacggcttga aaccgcgcca gacaaaccgg ggctgcgcat cagcgaattt gccgaatacg
    10861 gcaccttgat cggcggcggc ccacagggcg gcacagacgc cggcgccatc aagacccgcg
    10921 acacctggga tctggcgcaa gacccgcagt ctcaggccga actcggctgg cgcctgggca
    10981 gcacccttgc tgccctcaac ttcatcgtgc tgggcctggc cgtggccatc gtcaatccgc
    11041 gcgcccatcg cagcaccagc ctggtgctgg cgctgctggc cttcatggtg tactacaacc
    11101 tcatgaccgt gggtcaggcc tgggtgggcg ccggccgggt aggcatactg ccctttctgc
    11161 tggcgctgca tggcggcacg ctggcgctgg gtttgctgct ggtgaccgcc aggcacaacc
    11221 attggtcgct gcgcgccctg ctgccacgcg ggagcgctgc atgagaacca tacgccgctt
    11281 catctatcgc gaggtcgtgg tcagcgtggc cttcgtcacc ctggcctttc tggcgctgtt
    11341 tttcttcttc gacctggtgg atgagctgcg ctgggtcggg cggctgggca tgggttacca
    11401 gctcacgcat gccctgttgt tcgtggcgct gggtctgcca cagcacctgt atgaactgct
    11461 gcctatcacg gtgctgattg gcaccatctt cgtcatggcc aggctggcgc agagttcgga
    11521 attcaccatc atgcgtacca gcggcctggg gccctggcga gcactgagca ccctgctgtt
    11581 gctgggctgc gcctttgtcg cgctcacctt cgccgtgggc gactacctgg ctccgatcag
    11641 tgaacgtgcg gccgccctgg tcaaggcgcg cggccagggc cagatcagca ccggggccac
    11701 gggcgcctgg ctcaaggagc gcgaaggcga tcattccttc gccgtgaatg tcggcgccat
    11761 cgacggcaac gggcgcatgc gcggggtacg cgtattcgag ttcgatgaac gcggccgtat
    11821 cgcctccacc accaccgccg aacaagccga gtttgccgac aatggcgcgg gctggaatct
    11881 gcagcaggtg cagcgcaccg tattcgagca gcagaccgat ggcgacatga ccgtcaccaa
    11941 gctgcaggag ccgcgcttgc tgtggcagac ccgcatcagc ccggacatgg tggcggcctc
    12001 cctgctcaag cccgacaaga tggcgaccat cgagctgttt cactacatcc gccacctgga
    12061 ggccaacggc caggccgcgc aacgctatga gatcgagttc tggcgcaagg tgttctaccc
    12121 cttgagctgt ctggtcatgg tggtgctgtc gctgcccttt gcctacctgc atttccgctc
    12181 tggcggcatt gcgggctatg tgtttggcgg cgtcatggcg ggcatcagct tcttcctgtt
    12241 gaacaacgtc ttcggctttg ccggcaacct gcagaactgg tcgccctggc tgacggcggc
    12301 ggcgcctggc ctgatctaca gcgtgctgtc gctcgcggcc tttggctggc tggtgctgcg
    12361 gcgctagaga gtcttttaag tgttttcggc ctctaccacc catgcaacaa tcgcaattag
    12421 ctattatttt actagcacac ggctcgcgtg acccgctgtg gcgccggccc atagaggccg
    12481 tagccgcccg tatccagaca gaccagcccc ggctgttggt gcgctgtgcc tacctggagc
    12541 tgtgcgcgcc cgacctggcc ggcgccgtgg ccgagctggc gacgctgggc gcgacccaga
    12601 tcaccattgc ctccctcttc cttggtgcgg gccgccatgt gcgcgaggat cttccccgca
    12661 tggtgcagga tctggcccac aacttcggac acttgcagct acggctgcag cccccgatag
    12721 gcgaagatgc gcgcatgacc gcgctgatgg ccgacattgc ctcccagagc attgccagct
    12781 gagccataag acgcccaatc attagattca tgcaacataa tgacgcgatt ctttagccgt
    12841 cattcgatat gaaccttcac caattccgct tcgttcaaga ggccgcccgt cgcaacctca
    12901 acctgacgga ggccgccaag gcgctgcaca cctcgcagcc gggggtatcc aaggccatca
    12961 tcgagctgga ggatgagctg ggcgtggaca tctttgcgcg ccatggcaag cggctcaagc
    13021 gggtgaccga acccggccag catgtgctca agagcataga aatcatcatg cgcgaggtcg
    13081 gcaacctgaa gcgcattggc gagcagtaca gcgcccagga cagcggcacc ctggcgattg
    13141 ccaccaccca cacccaggcg cgctacgtgc tgcccgtgcc cgtggccagg ctgcgtgagg
    13201 cctaccccaa ggtcaacatc agcctgcacc aggccacacc gcacgaggtg gcgcgcatgg
    13261 tgatcgacga agtggtcgag atcggcatgg ccaccgaatc gctggccgac tatcccgacc
    13321 tggtcaccct gccctgctac gaatggcagc atgtgctggt gctgcctccg gcccacccgc
    13381 tggcgggcaa ggaacgcata gggctggagg acatcgcgca cgaggggctc atcacctacc
    13441 acccatcctt caccggtcgc ggcaagatcg accaggcctt cgccacacgc aaactgcagc
    13501 cgcgcatcgt gctcgaggcc atcgactccg acgtgatcaa gacctacgtg cgcctgggtc
    13561 tgggcatagg catagtggcc gaaatggccg tgcgcgacga ccccgtgggc gacctggtgg
    13621 tacgccccgt gggccacttg tttggccaga gcgtggcacg cgtggcgttc aaacgcggcg
    13681 cctacctgcg caacttcgtc tacaggtttg ccgagctgct cagcgaccgc ctgagccgcg
    13741 agttgatcgc ccgcgccatg accggccatg tcaacgacta cgaactctga acagaccgtg
    13801 aagcaactca caccgcccgc ctttcccagc cgcctgccga acgtgggcac gaccatcttc
    13861 accgtcatgt cggccctggc ggccgagcac caggccgtca acctcggcca gggctttccc
    13921 gacttcgcct gcgacccggc gctgacggat gccgtcaccc gcgccatgca nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggcggcgc gcgccttcat cgtctccagc
    14101 ttcggcaaga ccttccacgt cacgggctgg aaggtgggca ccgtggccgc gccggcggcc
    14161 atgacggcgg agttccgcaa ggtgcaccag ttcaacgtgt tcaccgtcaa tacgcccatg
    14221 cagtacggcc tggccgagta cctgcaggac cccacgccct atctgcaact gcccgccttc
    14281 taccaggcca agcgcgacct gttccgccag gggctggcgg gctcgcgcct gaaactgctg
    14341 cccagcaccg gcagctactt ccagtgcgtc gatatttccg cagtcagcga cctgagcgaa
    14401 gccgacttct gccaatggct cacgcgcgag atcggcgtgg ctgccattcc gctgtcagcc
    14461 ttctatggcg acggtttcga tcagcgcgtg gtgcgttttt gctttgccaa gaaggatgaa
    14521 acgctgcgta cagcaataga gcgcctgcgc cggctgtaaa cccacgggcc tgccatgcgc
    14581 gccatcctgc tgctgattgc cgtaatgatc agctacggct cgctctaccc gttccacttc
    14641 gcggccaacc accattggct ggcggaagct gccggactgt tcagcatgcc cacgctgcgc
    14701 atgggccgcg gcgatctggt cggcaacctg ctgctgtttg tcccatatgg gcttgttgcg
    14761 gctctgcatg tcgatacacg caggccagtg cgtgccagcg gcacactggc cctgctgctc
    14821 ggtctgggcc tgctgctggc gctcgccttg caactggcgc agatctgggt gcccagccgg
    14881 gtaccagaac tcaacgatgt catagccaat ggcgtcggca tggtgctcgg cacccttgcc
    14941 ggatgggtgg cacgcagtct ttttcccaac gctgccatgc agctgccgcg tgcgctggca
    15001 cccgccatgc tgatgctgct ttggatcggc tatcaatggg ctccgctggt gccaacgctg
    15061 gatctgcaga acatcttgaa cgcgctgaag cccctggtgc actcgcccca ggtcgatgcc
    15121 gtgcgggcct tgcacacgac cctggcctgg ctggccttct tcaaactgtg ggagctcgcc
    15181 gctggcaaaa ccctgtccac cccggtcatg gcgatggcag cccttggcat cgtcggcgcc
    15241 aagttgttca tcgtcggcgc cagcatctca gcgacgaatg cgctcgcgct gggcctggcc
    15301 ctggtttgcc tgccctggcg ccagcacgcc atggccgtgc cggtgctgag catcgccatg
    15361 ttcatcagcc tggcggcctc gggcctggcg ccgtatgaac cgctgggcca ggcgcaggcg
    15421 ttccactgga ttccgttcac cggcatgctg caaggcagca tgggcaccaa tctgctgaac
    15481 ctggccgaga agagcttctt ctatggcgcc ttgatcatgc tcatcggagc tccgctcagc
    15541 caccccctgg ccgcggccgg catggtcgcg ttcttcctcg gcttgatcga ggcggcccaa
    15601 ctctttctgc ccggccgtgt cgcggagtca accgaccccg tgctggccct catcctcggc
    15661 ctggtgatac ggctgctgtc ggttcgcgga aaaccggcct gacgcttaca gcagcacgat
    15721 gtcgtactgt tctattcttg caatttaatt catttcattt caataacttg cgaaagtttt
    15781 tactccaaaa aatcagccta actacacatt tagctacaca ccagggctct atgccgtttc
    15841 gtgtggaact tggcaatacc tgccaagtgc ggcggggagc cttggagtac aagagctgac
    15901 attggacgac aacattggaa acatgatcgt gcccaaagca gcagtcaagg attcacaagg
    15961 cttgtcgcct caacatgttg attcgacaaa ccttttccca tggtttgcca tgatttgacc
    16021 gcatcaaggc caagccctgc gggtggacta cgtccagcct tgacccggtc aaatcatggc
    16081 ggcgagacgc cccaaaccat ctgccccacc tttgcaactt ccacacgaaa cgacgaggag
    16141 ccatccttga gctgatcttt gggaacaaca aaaagcgcaa agacaaatcc acgctggttg
    16201 aggatgtgcg ccgcgtcata cgcgccagca ttggcaaccg cgccaaggaa gacttggtag
    16261 tggaatttat caaccacacc aacttggatg agctgggcga caaggccacg gtgattgatg
    16321 ccttcttcca atatgcccag gctgagcagc agcgtgaagc gcaggcgttg attgatgagg
    16381 agaagctgaa tgtggaagct gccaaacgct acatcaccaa ttccttgaaa cgtgaattcg
    16441 cctcagacag cggcactgaa ctgaatgctg tgctgcccaa gatgagccct ctgaacccac
    16501 agtacctcac caagaagcag acggtgtttc agaagatcgc tgcgtttgtg gagaagttca
    16561 agggtgtggg tgggcaggtt tgaatctgcc cttttctcgc cggaaaatcc atagactgca
    16621 agaatgacag caaggaggtt gggatcgcac gtagggctgt gtgcccccca tcctgcagcg
    16681 tgacctgatt gggtcaaggc cgagttgtca tgcaggcagt ttggctgcaa gcagttcgac
    16741 cttctcgata ttgggcggag aactgagcaa ggtcgctgcg ttggccatca aggccgcggc
    16801 gatctggccg ttcagatgtg cctggcgacc tgcctcatcg gcaaaggcat cgaacacacc
    16861 gaacgtcgaa gggccaaact tcaatgcgaa ccaggccgtg gtgccggact cggcgttggc
    16921 gagcggcagt gcgctggcca ggaagtcggc aagcgcagcc tcctggccgg gtttagcttc
    16981 gaggcgaaca aacagggcga gcttggtcat gatgtattcc tttgggtaaa tggaaaagtg
    17041 ggtgaaggga tcgacgagac tgcagtctaa gtcttcatga cagacatctc tataggcaat
    17101 aatgacaaca ttgatatcat tgttgccatg aaactccacc tccttgtttg tgatggcgtg
    17161 tttgatctgg ggcttgcggc gctcactgac acagtgggct tagccaatgc gatggcgggc
    17221 tcgctgccac aggctcccgc gcacatagag ctcacgctgg tgggcgtgcg gcgtcgcatc
    17281 cgaactgcgc aaggcttgac ggtccccgtg gtcactgcgc atggtgtgcc cgaaccagac
    17341 gtcgtgctcg tgccagcgtt tggcgacaag atgcctgaca cgctctcggc tcgactcaca
    17401 cgccccgatg tgcccgacgc ggtcgcggcg ctacagcact ggtccaccgc tggcgcgcac
    17461 cttggtgccg cttgctccgg tagtttcttg cttgcagaga gtggcctgct tgatgggcat
    17521 cgtgcgacga cgtcatggtg gctggggccg atgtttcggc agcgctatcc gaacgtcacg
    17581 ctggacgagt cacgcatgat cgtgaactcg acacgcttca ccaccgcggg tgccgctttg
    17641 gcccacgtcg acttggcctt gcgcatcatc cgagggcgca gtccggcgct ggcggccctg
    17701 gtggcacgct atctgctggt cgagacacgc agttcgcaag ccgagttcgt gattcccgac
    17761 caccttgcgc atgccgaccc gatggtggag cgcttcgagt gctgggccag acgtcggctc
    17821 gcgcaggggt tctcgctggc cgaggcggcc agcgccgcgg gcacaagcga gcgcaccttg
    17881 gcgcggcggc tgcaaagcgt tttgggaaag acaccgctgt cgtatttcca ggacctgcgc
    17941 gtggagcatg ccgtccatct cttgcgcacc gggaacgcca gcgtcgacca ggtcgccgca
    18001 caggtcgggt actcggatgg ggtgaccctg cgggccctgt tgcgccgcaa gctgggccgg
    18061 ggtgtcaggg agttgcgacg cggtggatga ccaaaggcgt ttggtgcatg tacggctgga
    18121 gaccgccgcg aaactgtcgg aagcaaaggc ttacccactt gcgtctacta catcgtgcgt
    18181 tgggtgcaaa agcaccagcc gtgccaaagc agtcttcagt gacctttcaa agttcagaga
    18241 cctttcaaag ttcagagacc tttcaaagtt ctgagtattg ggaatactgt tagctttacc
    18301 actgttttct gttcactaat ctggcttttc atccacatgc tattcaatcc acttcaagta
    18361 ggttctctca ccctgcccaa ccgcatcctt ctggcaccgc tgacccgcac ccgtgccgac
    18421 gctggccaca tgcccaacgc gctgatggcc gagtactaca gccagcgagc caccggcggc
    18481 ctgctgattt ccgaatgcac catggtggcg cccggtacat cggccttcgt caatgagcct
    18541 ggcatctaca acgacgcgca gattgcagcc tggaggcagg tgaccgacgc ggtgcacgcc
    18601 aagggcggac gcatcttcat gcagatctgg cacgctggcc gcgcagccta ccctggcgct
    18661 gccgatggcg cccccattgt ctccagcagc gccaccgcta ttgagggtga gattcacacg
    18721 ccccaaggca aggtgccgca cgcggtgccc cgccccctga ccgccgaaga gattcccggc
    18781 atcgtggctg cgtttgccca aggcgcacgc aacgccattg ccgccggctt tgatggtgtg
    18841 gaagtgcacg gcgccaacgg ctacctgatc gaccagtttc tgcgcgatac gcccaaccag
    18901 cgtaccgatg cctatggcgg ttcgctggaa aatcgtgcac gcttgctgtt cgaggtactc
    18961 accgccgtga cccaggccat cggttccgag cgtgtgggtc tgcgcctgtc gccactcaac
    19021 agctacaaca gcatgaagga cagcgatccg ctggccctca tcggtttctt ggccgacaag
    19081 ctcaacgcct tcaagctggc ttacctgcac gtgatgcggg ccgacttctt cggcgtgcaa
    19141 aaggccgatg tcatgcccgt agcgcgtgag aagtacaaag gcgtgttggt gggcaacatg
    19201 ggctacagcg ctgacgaagc cgaggcggcc atcgccgcag gcaagcttga cgctgtggcc
    19261 tttggcaccg ccttcctggc taacccggac ctgcccacac gcatcagtgc caaggcaccg
    19321 ctgaacgcgc ctgacaccaa cacgttctac tcaggtggcg ccaagggcta tacagactat
    19381 ccgacactgc aggtcgcgta aatcaccgcc ccagaagacc gaagaaacga gccactttat
    19441 acccaaggct ctgcccttcg atcttgcgaa gctgtgaatc gatggcgtct accgagtgtt
    19501 gtgcgccatc cactgcttca actgcagatc ggacagcaac ccacttttga tcgccatatc
    19561 agtgctcctg gcgccacgcg ggggccgggt ccagcgcacc ggctagctac ccacgcagct
    19621 acccaaaatt gttgcgaact gatgggaccg gaagggacag catgaaatgc ttgcctgacg
    19681 aaatctcctt taacgacagt aacttacgta tggcgaatgg gacggtgtga gccccccaac
    19741 gatcacagca gaacaatgtc gtactgctcc tgcccaatgg tgctttccgc ctgcagcgag
    19801 atcggc
//
LOCUS       DAOMTQ010000043        19466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_349_length_19466_cov_28.278193, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000043 DAOMTQ010000000
VERSION     DAOMTQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 19466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19466
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_19466_cov_28.278193"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK518_06210"
     CDS             complement(<1..346)
                     /locus_tag="PK518_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRM48542.1"
                     /translation="MGPASTTGDKHGNPQSLPCALQRGSGPGQERNWGIFSIFNVWTS
                     DVHSLWGYYLAASLFLLCGSFTNFLLAIGLGSLVIFVLMNLIGWAGEKTGVPYPVLAR
                     ASFGIWGANLPAI"
     gene            596..1444
                     /locus_tag="PK518_06215"
     CDS             596..1444
                     /locus_tag="PK518_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRM48543.1"
                     /translation="MTISTVGIIGAGTMGNGIAQACAVSGINVVMVDISDAAVQKGVA
                     TVAGSLERLIKKDKITAADKDAALARIKGSTSYDDLKAAQIVIEAATENHELKLKILK
                     QVDAIVAPEVIIASNTSSISITKLAAATGRAERFIGMHFFNPVPMMALVEIIRGLQTS
                     DATHAAVKELAERLGKSPITVKNAPGFVVNRILVPMINEAFFVLAEGLATPEDIDAGM
                     KLGCNQPIGPLALADMVGLDVCLAVMDVYLQEFGDSKYRACPLLKEYVAAGRLGRKTG
                     RGVYQY"
     gene            complement(1496..1771)
                     /gene="pqqD"
                     /locus_tag="PK518_06220"
     CDS             complement(1496..1771)
                     /gene="pqqD"
                     /locus_tag="PK518_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HRM48544.1"
                     /translation="MDSTTAPRPQLHPRFIMRWEASQDAHVILYPEGLIKLSPSAAEI
                     LQRCDGTRTDDKIIDDLAQAYPSQREAIAQDTASFLAVARDKGWILE"
     gene            complement(2013..2834)
                     /locus_tag="PK518_06225"
     CDS             complement(2013..2834)
                     /locus_tag="PK518_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48545.1"
                     /translation="MNARIPLAPSPATWLQRPGLAGGIDQDGGRAAELLGHGFGSVEF
                     GSVTAQPLPGHNVGVAALVQALKNALAAAPQSAPQGRAPCAVGIGLGVPAAVALEHVA
                     AQWLPGFAAAAEVAHYVSLNLSARAHQRLLEPAGLAVLGAAFVAVATLRDQTHQLGRE
                     GQHGAVRLAIKLPGTHPGASTAAALALAAGIDQLTVVLPDTGERWACLSALRQQVGAG
                     PDLVAVGGIRCRADVDRARACGASGVQVHRLFTAQGAQCLQGLIQPTCSDGDADS"
     gene            complement(2831..3910)
                     /gene="serC"
                     /locus_tag="PK518_06230"
     CDS             complement(2831..3910)
                     /gene="serC"
                     /locus_tag="PK518_06230"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRM48546.1"
                     /translation="MPYSFAAGPARLPDAVLAQARETLFSRGADGAAPVERPFSAPQF
                     RAALAHAQARLRALLGVPDGYRILFLAGGAMHQFAMVPANLATPGGMVAYADSGYWAQ
                     RAMAEAARQHQVCVVARHEGTQALAAPPLQGWQLPPGSAYCHITPNETVEGIAYPGLP
                     DVGDVPLVADCTSSFLTAPLPIARFGLVYAGAQKNLGIAGLAVVVIQEALLARSSASL
                     ASVASYRLQAQAASCVNTPPVAALLVTAMVLDWIDAQGGLPAMGEANQRKAATLYCAI
                     DASQGFFCAPVALGHRSLVNVRFCCANAALDTVFATEAEAAGLHHLRGHPRIGGLRAS
                     LYNAMPQAGVDALVDFMAHFQRRYG"
     gene            complement(3920..4426)
                     /locus_tag="PK518_06235"
     CDS             complement(3920..4426)
                     /locus_tag="PK518_06235"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRM48547.1"
                     /translation="MDMAMLDLLARWLHFLAGVIWVGHNYASVVNRPRWRPLAASDLS
                     DPDSPKLKEVINREHGTFRWASVVTWCAGIFMLWHRGWLPGALQLQGTLAPIGVGVYI
                     GTLMMLNVWFVLWPHQKKVLGLVPAPVHERLRCSRITHLSSRTNTMLSIPLLFLMASG
                     SHGGYLLR"
     gene            complement(4528..5673)
                     /gene="pqqE"
                     /locus_tag="PK518_06240"
     CDS             complement(4528..5673)
                     /gene="pqqE"
                     /locus_tag="PK518_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014726238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HRM48548.1"
                     /translation="MPKEEQGLSLDGQGKPLWLSLELTYRCPLKCSWCNNPLNFDDYT
                     SQELSTQEWKRVLREARDLGALQLGFTGGEPLMRDDLEELVAYADSIGFYTNLITSGV
                     GLNEERLVALKAAGLKQIQLSVQSTRAEMTDALVGARAHALKMDAARRIKAHGFPMVL
                     NMPVFKQNIDLIGEMCEWAADLGIEYIEFANIQYYNWALLNRDELLPSLEQIRQAEAT
                     VNAWRAKLGKRMTLYFVVPDYFEDRPKACMNGWGAIHLTIAPDGVAMPCQESRVIPGL
                     EFESVRERSLAWLWHESPLFRKYRGLDWLPEPCQSCSEKEKDFGGCRCQAFLLTGDAG
                     NTDPACSRSPFHHRITEAVKEVVRPLRFKKPLVKRSASAVSTTFMEE"
     gene            complement(5788..6435)
                     /locus_tag="PK518_06245"
     CDS             complement(5788..6435)
                     /locus_tag="PK518_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM48549.1"
                     /translation="MSPNLHATRIMLADDHAVVRLGLRLLLEGAGATVVGEAESGETA
                     SRMYGALDPDVLVMDISMPGIGGLGALERILSHAPKARVLMLSAHSDDIMPTRALRMG
                     ARGYLCKRAAPDEFLRAVAAVAKDQRYIDPELAAALALAQLSKSSSPVETLSEKELAV
                     FLQLASGRSVQQVAEDFRLSPSTVGTHLYHIKQKLNVQNAAELALVALRNGMIDV"
     gene            complement(6432..7799)
                     /locus_tag="PK518_06250"
     CDS             complement(6432..7799)
                     /locus_tag="PK518_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM48550.1"
                     /translation="MSIPPRSLHTRISLLLTGLAAILLMVLASLWVHGMRSSIHEEIS
                     AANRVSVQWLKALASDVQSAPPEFVAQRVLSMVRPLGRIRANALEVFTEDGRRLYASP
                     APTYKADRTAPGWFTALVAPVFAVHRVGVGNLELVISPDHSRAVVDAWDDLQVLCLWA
                     VALLGALFALVRWALGRTMRPLEQVMDALDCTGGGRFDVRLPVFPLRELGRLSLAFNG
                     MADRLEEAVEDNVRLKSEREVACLVQARLEDERRAIARELHDTLAQCITGVRALAGAI
                     AQRTSDQPALQQPAQTIVAVTGEMQDGVKAILYRLRSALEHDLGTDIAQACTRWELLH
                     PHIALNCRMDIGTTPLAAELTQTAVRVVQEGLTNIVRHAKATQADLCIERSGGWLNIR
                     LSDNGCGLGSGTGHPGSGLGLTGMRERIAAQGGQLELNTAASGGLCVLARLPDHPLSA
                     LESAA"
     assembly_gap    8092..8191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8192..9174)
                     /locus_tag="PK518_06255"
     CDS             complement(<8192..9174)
                     /locus_tag="PK518_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48551.1"
                     /translation="MNPIHRRACLALAACGALAALAAPGALAQTYPAKPITMVVPFPP
                     GGPTDMVARLLAQKLSEQMGQPVVVDNKPGANGNIGGALVSKAAADGHTLLYNTSSIT
                     LSPALYKSMQYDVLRDLAPVALTAVVPLALVVNPAVPANSVQEFVAYAKANPGKLSYG
                     SAGNGNVTHLAAYQFVRAQGIEATHVPYRGSAPADVDLAAGQIQFMTDTINSVMAFVK
                     DRRMKLLAVTTAKRMSLFPEVPTLAETVMPGFEAGAWQGLMVPAGTPAAVVQRLNAEV
                     NKALQSADMRDKLAVQGAEPLGSTPEEYGAYVRKELARWATVVKATGVTLE"
     gene            complement(9198..9662)
                     /locus_tag="PK518_06260"
     CDS             complement(9198..9662)
                     /locus_tag="PK518_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRM48552.1"
                     /translation="MDHAAPRLGQGFVWQDLQVGQRFRTFRRTVTESDLVQFINVTGM
                     LEAIFIEEGYEGGAMRGRPVPGALTYTLIEGFILQTMIQGTGLAMLELHQKILAPVLV
                     GDTIEAMVEVTGIRPTSKSGRAVVTSRIDVFKQGGVQVMTYTATRLLAGRTQ"
     gene            complement(9677..10882)
                     /locus_tag="PK518_06265"
     CDS             complement(9677..10882)
                     /locus_tag="PK518_06265"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM48553.1"
                     /translation="MKPLQGLRVLDLSSVIFGPLASQVLADYGAEVIKVEPPGGDSTR
                     HTGPAPEPGMAAMFLGSNRSKKSIALDLKRPEARQALQALIATADVFMHSMRPQKLAG
                     LGVDPETLRARHPRLVYASLLGFLPGPYAGRPAYDDVIQGMSGLADLMEQQGGEARYL
                     PTIAADKTCGHVAAHAILAALWQRERTGEGAFVEVPMFESMVGFNLVEHFYGQHFEPP
                     RSAPGYPRVLAPWRRPYHTADGLVAMMPYTNLHWQRFFAEVGAPQHADDPRFSDIASR
                     TAHIAELLELASTYVRRQPTAYWLATCERLEIPAAPVARLAELRADEHLLATGFFEEL
                     QDAAMGTLRFPGVAVRFDGQRPPVTMAPRLGEHTRALLAEVGLDAAQVQALIDQGAAI
                     APQHKDADT"
     gene            11081..11839
                     /locus_tag="PK518_06270"
     CDS             11081..11839
                     /locus_tag="PK518_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRM48554.1"
                     /translation="MKIALLSDLHANLPALDACLEHARAQGAEQFALLGDLVGYGADP
                     GPVLDRVMQLVADGALCVRGNHDDAAVAPPPQVENLGDQSAQWTHPHLSAEQRAFLAG
                     LPLTARMGPDALLVHASADGPERWHYVADSNAAERSMAAATQIDPAIRYVFSGHVHEQ
                     ALYFLTPTAKLMRFSPQPGVPVPVPPHRQWLAIVGSCGQPRDGDTRAMYALFDAGAAT
                     ITFQRVRYDHMAAAAAVRASGLPAFFADRLEKGR"
     gene            11844..13274
                     /locus_tag="PK518_06275"
     CDS             11844..13274
                     /locus_tag="PK518_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HRM48555.1"
                     /translation="MKLLEPGTEVDGFVVHECIHAGGMAHIYHAGYANTARDPGFPLA
                     MKIPRMTAGDGAENIVSFEVELTILPTLTGHHAPRFVAAGDLMRLPYLVMEYVEGQTL
                     QHWLDSHQRGDAEQDARDIARIGSALAIAAHSIHQQNVCHLDLKPANVLLRPDGSVVL
                     LDFGLSCHAHYPDLLAEEMRKAVGSPTWIAPEQVVGVRGDLRSDIFAIGVMLYEMATG
                     ELPFGSPTTDAGLRQRLWMTPAPPRQHRPDLPEWLQEVILRCLEPEAAQRYPSAAHLA
                     FDLANPEQVPLTERGRRLRGPGLRVHFKRWIKAAGMHYQPSPLPTRQIEEVPILMVAV
                     PHDDVTDATLYSLREAAARSLGIRPGARLACVSVISPSASSASDSERSETTLRRQHLA
                     RLKQWATGLDLSRHSASYHVLESSDVAQALVRYAEANRVSMMILGAATHGLQLQRFVA
                     TVPMRVARDAPCTVILVKQQLPFEHL"
     gene            complement(13281..13721)
                     /locus_tag="PK518_06280"
     CDS             complement(13281..13721)
                     /locus_tag="PK518_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48556.1"
                     /translation="MARQINETGWRGAAALAMAAFLAACASPSAHVGVGVPVGPVSIG
                     VGLGSGGVSAGVSTGVGPLGVGVGVNQRGQVTGGAGVGASVPMGNARVGVGVGSSTVL
                     HDPQQPAATPGAAPPPVPGAQGAPLQWRDAQGRPVPECRVMGGC"
     gene            13774..14826
                     /locus_tag="PK518_06285"
     CDS             13774..14826
                     /locus_tag="PK518_06285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRM48557.1"
                     /translation="MHAMTATPADTAPPHPYASLTPDLVLDALASVGLYGDGRLMALG
                     SYENRVYQVALEDGSRVVVKFYRPGRWSQAQIEEEHAFALELAAAEVPVVAPLMLQGR
                     SLHRHAGFGFAVSPWRGGRMPELDDFEVLEWLGRYLARIHNVGAQRPFEQRPRLDAQS
                     FGQEPRDWLLSQQIIPLDQQSAWHAACDEALELIAVDAYPSSATGQFGLKDANAIRLH
                     GDCHPGNVLWTPVDDAGQGGPHFVDLDDARMGPAVQDLWMLLSGDRRQRALQLSTLLD
                     GYEQLRAFDRRELALIEPLRTLRLIHYSAWLARRWSDPIFPINFPWFGSSDYWRGQVG
                     MLREQIEAMREEPLTA"
     gene            complement(14852..15844)
                     /locus_tag="PK518_06290"
     CDS             complement(14852..15844)
                     /locus_tag="PK518_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48558.1"
                     /translation="MRIKTVLATMALALGAAGWAQAQTASAAKDYPNRPITLVVPAGA
                     GGGTDVVARVLADQLGKRLGQAVVVDNKTGASGMLGTQAVARAAPDGYTLLVAYSTPV
                     FYAHHIFAKVPYDIRKDFEFITQLASTSLVMMVNANVPVKNMKEFMAWAQANKGKVNY
                     GSYGQGSAGHLMSAYLSDTRKLDMTHVAYKSESPHIQDLAGGVVSWGLGTIAAAQGVL
                     KDNRVRPIAIYGPKRLAELPDVPTMAEQGFADPEFNTVAWFTLLAPKGTPKPILQRLE
                     KESVEIIHSTAMKARFQVFGLESVPGGAAQFHKDFDAVDPVIQKLVKISGVKAE"
     gene            16385..18070
                     /gene="lldP"
                     /locus_tag="PK518_06295"
     CDS             16385..18070
                     /gene="lldP"
                     /locus_tag="PK518_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRM48559.1"
                     /translation="MQTLWQQNYDPAGNIWISALVALIPIIFFFLALTKLRLKGYQAG
                     TITVLLALGVALLFYKMPVSAALASAVYGFFYGLWPIAWIIVAAVFLYKISVKTGQFD
                     VIRSSILSVTPDQRLQLILVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGL
                     CLIGNTAPVAFGAMGIPIIVAGQVSGVDAFAIGQMAGRQLPFMTVIVLFWLMAIMDGW
                     RGVKETWPAVLVGGGSFAVMQFLTANYIGPELPDITSSIVSLVALTAFLKVWQPKRIF
                     RFETESQPGTKPGASASAPATAHHPLTAVAIVKAWSPFIILTAMVTLWSLKPFKALFA
                     AGGPLASTIINIPVPMLHNLVEKAPPVVAAATPYGAVYTFNWLLATGTAILIAAIITI
                     AFTRLSPAKAVATLGETFKELLVPIYSIGMVLAFAFVANYSGLSATLALALAHTGHAF
                     TFFSPFLGWIGVFLTGSDTSANALFGALQATTAQQLGLPQVLTVAANTTGGVTGKMIS
                     PQSIAIACAAVGLAGKESDLFRFTVKHSLIFAVIIGIITTLQAYVFTWMIPGH"
     gene            18103..18891
                     /gene="lldR"
                     /locus_tag="PK518_06300"
     CDS             18103..18891
                     /gene="lldR"
                     /locus_tag="PK518_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HRM48560.1"
                     /translation="MRLADHVVEKLLALVQARGLQPGQRLPAERQLAEELGVSRTSVR
                     EAIQKLTSQGVLSARRGDGTYLQSRATAEWLQEGMQPLAGLIDADPHYRYDVLEARQA
                     LESSAAWLAAQRATAQDKARIQHCFEVMLDHQQNGHAELAARADAQFHLAIAEASHNL
                     VLVQVTHSLFTTVLSTVARNRHDMFHLSAPVTVQALTEQHQALMQAILDGDPQRARQC
                     IDEHLEHVRVTIQRLDEDRARQERSTRLPPDLVPPLLPETSPHT"
     gene            18902..>19466
                     /locus_tag="PK518_06305"
     CDS             18902..>19466
                     /locus_tag="PK518_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRM48561.1"
                     /translation="MIISSSADYRAAAQKFLPPFLFHYIDGGAYAEQTLRRNVDDLAS
                     VALRQRVLKDMSQLDTGIELFGEKLAIPVALSPVGLTGMYRRRGEVQAARAADAHGIP
                     FTMSSVSVCPIEEVVPKIKRPMWFQLYVLKDRGFMQNALERAQAAGCSTLVFTVDMPV
                     PGARYRDAHSGMSGPNAPLRRYWQAMTH"
BASE COUNT         3215 a         6718 c         6306 g         3127 t
ORIGIN      
        1 cgatagccgg caggtttgcg ccccagatgc cgaacgaggc ccgcgccagc accgggtacg
       61 gaacaccggt cttctcgccg gcccagccga tcaggttcat cagcacgaag atgaccagcg
      121 agcccaggcc gatggccagc aaaaagttgg tgaagctgcc acacagcagg aacaggctgg
      181 cggccaggta gtagccccac aggctgtgca cgtccgaggt ccagacgttg aagatgctga
      241 agatgcccca gttgcgctcc tggccggggc cagatcctcg ttgtagagcg caggggaggg
      301 attgtggatt tccatgcttg tcgccggttg tcgatgcggg tcccaagcac aactcagcca
      361 cgccttgttg tgttcaatgt ttgcgagatg gtatacaaga tggtacgcaa cgacaccagc
      421 cgaatgatca gggtttgcct ggatgcgtca tgcaacatcc tgtatccagc ccgatctggc
      481 agccacggcg cgcgccccgg cgatactgcc cggtacgccc cggaaaagag ggcaccgcga
      541 ttgccgatac catcggcgca tccgtttctc tcatcactcc ttcgaggact cttttatgac
      601 gatttctacc gtcggcatca tcggcgcggg caccatgggc aatggcattg cccaggcctg
      661 cgcggtctct ggcatcaacg tggtcatggt ggacatctcc gacgccgccg tgcaaaaggg
      721 cgtggccaca gtggccggca gcctggagcg gctgatcaag aaggacaaga tcacggcggc
      781 cgacaaggac gcggccctgg cgcgcatcaa gggttcgacc agctacgacg acctgaaggc
      841 cgcgcagatc gtcatcgagg ccgcgaccga gaaccacgag ctcaagctga agatcctgaa
      901 gcaggtggac gccatcgtcg cgcccgaggt catcatcgcc tccaacacct cgtcgatctc
      961 gatcaccaag ctggccgccg ccaccggccg tgccgagcgc ttcatcggca tgcatttctt
     1021 caaccccgtg ccgatgatgg cgctggtgga gatcatccgt ggcctgcaga catccgacgc
     1081 cacgcacgcc gccgtcaagg agctggccga gcgcctgggc aagtcgccca tcacggtgaa
     1141 gaacgcgccc ggcttcgtgg tcaaccgcat cctggtgccg atgatcaacg aggccttctt
     1201 cgtgctggcc gagggcctgg ccacgcccga ggacatcgat gccggcatga agctgggctg
     1261 caaccagccc ataggcccgc tggccctggc cgacatggtg ggcctggacg tgtgcctggc
     1321 ggtgatggac gtgtacctgc aggagtttgg cgacagcaaa taccgcgcct gcccgctgct
     1381 caaggaatat gtcgccgccg gccgcctggg ccgcaagacc gggcgcggcg tctaccagta
     1441 ctgacgccgg ctactctgcc ggcgcaaggg aaagggcgtc gcaggagctg ttcaatcact
     1501 ccagaatcca gcccttgtcc cgcgccacgg cgagaaagct cgcggtatcc tgggcgatgg
     1561 cctcgcgctg gctcgggtag gcctgggcca ggtcgtcaat gatcttgtcg tcggttcgcg
     1621 tgccgtcgca gcgttggagg atttctgctg ccgacgggct gagcttgatc agcccttcgg
     1681 ggtagaggat gacatgcgcg tcctggctcg cctcccagcg catgatgaag cgcgggtgca
     1741 gctgcggacg gggtgcggtg gtgctgtcca tggggtgcag ggcctgccgg cgcgctagcg
     1801 gccgaagccg ctggggcgta ccggcttagc gcacgtaaac gtaggcggtc acttcaaagc
     1861 caagacggat ttcgcaatag gcgggggtat gccaggtcat gggtgtctcc tcgtgaggtg
     1921 ttgcgaagcc gcaccacgcg gcttctgggc atattgttcc ggggccggtg gttgcggtcg
     1981 tccgcgcttt atggggcggc acctccagaa aatcatgaat ctgcgtcgcc atcgctgcat
     2041 gtgggctgga tcagcccctg caggcattgc gcaccctgcg cggtaaaaag gcggtgcacc
     2101 tgcacgccgc tggcgccgca ggcccgcgcg cggtccacgt cggcgcggca gcgaatgccg
     2161 ccaacggcga ccaggtcggg gccggcgcca acctgctggc gcagcgcgga caggcaggcc
     2221 caacgctctc cggtgtcggg caacaccacg gtcagctggt cgatgccggc cgccagcgcc
     2281 agcgcggccg ccgtcgatgc ccccgggtgg gtgccgggca gcttgatcgc caggcgcacc
     2341 gcgccatgct ggccttccct gcccagctgg tgtgtttggt cgcgcagcgt ggccacggca
     2401 acaaacgccg cacccagcac cgccaggcca gcgggctcaa gcaggcgctg gtgggcacgg
     2461 gcactgaggt tcaggctgac gtaatgggca acctctgccg ctgccgcaaa gccaggaagc
     2521 cactgcgctg ccacatgctc cagggccacg gcggcaggca cgcccaggcc aatgccgacg
     2581 gcgcacggcg ccctgccctg cggcgcgctc tgcggggccg ctgccagtgc gttcttcagg
     2641 gcctgaacca gcgccgccac gccgacgttg tgcccgggca gcggttgtgc tgtcacgctg
     2701 ccaaactcca cgctgccaaa tccatggccg agcagctctg cggcgcgccc cccatcctgg
     2761 tcgataccgc cggcaagacc gggacgctgg agccaggtgg cgggactggg tgccagcggt
     2821 attcgtgcat tcatccgtag cgcctttgaa aatgggccat gaaatccacc agcgcatcga
     2881 ccccggcctg cggcatggcg ttgtagagac tggcgcgcag accaccaatg cgggggtggc
     2941 cacgcaagtg gtgcaagccg gcggcctcgg cttcggtggc aaacaccgta tccagcgccg
     3001 cattggcgca gcagaaccgc acgttgacca gggagcgatg gcccagcgcc accggtgcgc
     3061 aaaagaaccc ctggctggcg tcgatggcgc agtacagcgt ggccgccttg cgctgattgg
     3121 cctcgcccat ggcgggcaga ccgccttgtg catctatcca gtcgagcacc atggcggtca
     3181 ccagcagggc cgccaccggc ggcgtgttga cgcaactggc ggcttgcgcc tggaggcggt
     3241 acgaggcgac ggatgccagg ctggcggagc tgcgcgccag cagtgcctcc tggatgacga
     3301 ccacggccag gccggcaatg cccaggttct tctgtgcccc ggcatagacc aggccaaagc
     3361 gcgcgatggg caggggcgcc gtcagaaagg acgacgtgca gtcagccacc agcggcacat
     3421 cgcccacgtc aggcaggccg ggataggcga tgccctcgac cgtctcgttg ggtgtgatgt
     3481 ggcaataggc gctgccgggc ggcaactgcc agccctgcaa cggcggcgcc gccagcgcct
     3541 gtgtgccttc atgccgcgcc accacgcaaa cctggtgctg cctggccgcc tcggccatgg
     3601 cgcgctgggc ccagtagccc gaatcggcgt aggccaccat gccgccgggg gtggccagat
     3661 tggccggcac catggcgaac tggtgcatgg cgccaccggc cagaaacagg atgcggtagc
     3721 cgtctggcac gcccagcagc gcacgcaggc gggcctgggc atgggccagt gcggcacgga
     3781 actgtggcgc ggaaaatggc cgctccacgg gcgcggcacc atctgcgccg cggctgaaca
     3841 gggtctccct ggcctgcgcc agtacggcat cgggcaggcg tgccgggccg gcggcaaagc
     3901 tgtagggcat ggcgcggccc tatctgagca ggtacccgcc atggctgccc gatgccatga
     3961 gaaacagcag cggaatcgac agcatggtgt tggtacgcga ggagaggtgg gtgatgcgcg
     4021 agcagcgcag gcgctcgtgc accggcgccg gaaccaggcc cagcaccttc ttctggtgcg
     4081 gccagaggac aaaccagaca ttgagcatca tcagcgtgcc gatgtagaca cccaccccga
     4141 tcggtgcgag ggtgccctgc agctgcagcg cacctgggag ccagccccgg tgccagagca
     4201 tgaagatccc ggcgcaccag gtcaccaccg aggcccagcg gaaggtgcca tgttcgcggt
     4261 tgatcacctc cttgagcttg gggctgtccg ggtcgctcag gtcgctggcg gcgagagggc
     4321 gccaccgggg ccggttcacc acgctggcgt agttgtggcc gacccagatc acgcccgcca
     4381 gaaaatgcag ccagcgcgcc agcaggtcca gcattgccat gtccatggtg ctctcctagg
     4441 ccagcaggcg tagcagcgcc gccagcacgg ccgtcaaggc cacgcccagc gccacggtgg
     4501 tgagtgcgtt gtcgtaaggg tgtcgcatca ttcctccatg aacgtggtgg acacggcgct
     4561 cgccgaacgc ttgaccagcg gtttcttgaa gcgcaggggg cgcaccacct ccttcacggc
     4621 ttcggtgatg cggtggtgga agggcgagcg gctacaggcc gggtcggtgt tgccggcatc
     4681 acccgtgagc aggaacgcct ggcagcggca accgccgaaa tccttttcct tttcgctgca
     4741 ggactggcag ggctccggca gccagtccag gccacggtac ttgcggaaca agggcgactc
     4801 gtgccagagc caggcgagcg agcgttcgcg gaccgactca aactccagcc ccggaatgac
     4861 ccttgattcc tggcagggca tggccacgcc gtccggcgca atggtcaggt ggatggcgcc
     4921 ccagccattc atgcaggcct tggggcggtc ctcgaaatag tcgggcacca cgaagtacag
     4981 cgtcatgcgc ttgcccagct tggcgcgcca ggcattgacc gtggcctcgg cctggcggat
     5041 ctgctccagg ctgggcagca gctcatcgcg gttgagcaag gcccagttgt agtactggat
     5101 gttggcaaat tcgatgtact cgatgcccag atccgcggcc cattcgcaca tctcgccgat
     5161 caggtcgatg ttctgcttga acaccggcat gttcagcacc atgggaaagc catgcgcctt
     5221 gatgcggcgc gcagcgtcca tcttcagcgc atgggcgcgg gcgccaacca gggcgtcggt
     5281 catttcggcg cgggtggact ggacggagag ctgaatctgc ttgagccccg ccgccttcag
     5341 cgccaccagg cgctcctcgt tcaggcccac gccggaggtg atgaggttgg tgtagaagcc
     5401 aatgctgtcg gcataggcca ccagctcctc caggtcgtcg cgcatcaacg gttcaccgcc
     5461 cgtgaatccc agctgcagcg cgcccagatc acgcgcctcg cgcagcacac gcttccactc
     5521 ctgggtgctc agctcctgcg aggtgtagtc atcgaagttc agcgggttgt tgcaccagct
     5581 gcacttgagc gggcaacggt aggtcagctc cagggacagc cataacggct tgccctggcc
     5641 gtccaggctc agaccctgtt cttctttcgg catagcgctc ctttcggcaa cagcgacaag
     5701 atgcctcatt ctttccgccc tgcctgcagt gggctaccgg caaatttgca tttgcagctc
     5761 gcgaaaatca cgagaaccac atacggctca aacgtcgatc atcccgttgc gcaaggccac
     5821 cagcgccagt tcagcggcgt tctggacatt gagcttttgc ttgatgtggt acaggtgggt
     5881 gcccacggtg ctggggctca ggcgaaagtc ttcggccacc tgctgcacgg agcgccccga
     5941 ggccagttgc agaaagactg ccagctcctt ctcggacagc gtctccacgg ggctggacga
     6001 tttcgacaac tgcgccaggg ccagcgccgc ggccagttcc gggtcgatat agcgctggtc
     6061 ttttgccacc gcagcgactg cgcgcaaaaa ttcgtcgggg gccgcccgct tgcacaggta
     6121 gccccgggca cccatgcgca gcgcccttgt gggcatgatg tcgtcgctgt gtgccgagag
     6181 catcagaacc cgtgccttgg gggcatggct gaggatgcgc tccaacgccc ccaggccacc
     6241 gatgccgggc atggagatgt ccatcaccag cacatcggga tccaatgcgc catacatgcg
     6301 cgaggccgtt tcgccagact cggcctcgcc caccacggtg gcgcctgcgc cttcgagcag
     6361 cagccgcagg cccaggcgca cgaccgcgtg gtcgtcggcc agcatgatgc gcgtcgcgtg
     6421 caaattgggg ctcatgcggc agactccaaa gcagacaggg gatggtccgg cagccgtgcg
     6481 agcacgcaca agccgccgct ggcggcggtg ttgagctcca gctgaccgcc ctgcgcagca
     6541 atgcgctcgc gcatgcccgt caggcccagc ccgctgccag gatggccagt gccgctaccc
     6601 aggccacagc cgttgtcgct cagccgaatg ttgagccagc cgccgctgcg ctctatgcac
     6661 aggtcggcct gcgtggcctt tgcatgccgc acgatgttgg tcaggccctc ctgcaccacg
     6721 cgcaccgcgg tctgggtgag ctctgcagcc agcggcgtgg tgccgatgtc catgcggcag
     6781 ttcagggcaa tatgggggtg cagcagctcc cagcgtgtgc atgcctgcgc aatatcggtg
     6841 cccaggtcat gttccagcgc cgaacgcagg cggtacagga tggccttgac gccatcctgc
     6901 atctcgccgg tgacggccac aatagtttgt gcgggctgct gcagcgcagg ttgatcagag
     6961 gtgcgctgag caatggcgcc ggccagtgcg cgcacgccgg taatgcactg ggccagcgtg
     7021 tcatgcagct cgcgggcgat ggcgcgccgc tcatcctcca ggcgcgcctg aacgagacac
     7081 gccacctcgc gttcgctttt caggcgtacg ttgtcctcca cagcttcctc caggcggtct
     7141 gccatgccgt tgaaggccag cgacaggcgg cccagctcgc gcaacggaaa gaccggcagg
     7201 cgaacgtcga accggccgcc accggtgcag tccagcgcgt ccatcacttg ctccagcggt
     7261 cgcatggtgc gccccaatgc ccagcgcacc aatgcaaaca gcgcacccag cagcgccacg
     7321 gcccagagac acagcacctg caggtcgtcc caggcatcga cgacggcgcg cgagtggtcg
     7381 ggcgagatca ccagctccag gttgcccacg cccacgcggt gcacggcaaa caccggggcc
     7441 accagcgcgg tgaaccagcc cggtgcagtg cggtcggcct tataggtggg cgccggcgag
     7501 gcgtagaggc gccggccgtc ttcagtgaac acctccaggg cattggcgcg gatgcgcccc
     7561 aggggtcgca ccatggacag cacacgctgc gcgacaaact cgggaggcgc cgactgcaca
     7621 tcggaagcca gggccttgag ccactgcacg ctgacccggt tggctgcgga gatttcctcg
     7681 tggatgctgc tgcgcatgcc atgcacccac agtgaggcca gcaccatcag caagatggcc
     7741 gccagcccgg tcagcaaaag gctgatgcgt gtatgcaggc tgcggggcgg aatgctcatc
     7801 gcctctcctt ggctcaaata ctccggttcg gattgcatac cgcaccgcaa tcaccatgcc
     7861 aaagatctct gtccccatgg gcgcacccag ccaccggctg tggtgcgacg caatacaaca
     7921 actggtgatt tgtggagcaa tccgttgtgt gccgaagtga cacacccgga cgccagggcc
     7981 gcccttgggc ggcgtcatca ccaggcgtcg tttacaaggc gccgttcacc gggcgctact
     8041 ccagcgtgac gcccgtggcc ttgaccaccg tggcccagcg ggccagctcc tnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntactccagc gtgacgcccg tggccttgac
     8221 caccgtggcc cagcgggcca gctccttgcg cacataggcg ccgtattcct cgggggtgga
     8281 gcccagcggc tcggccccct gcacggccag tttgtcgcgc atatcggcgc tttgcagcgc
     8341 cttgttgacc tcggcattca ggcgctgcac cacggcggcg ggcgtgccgg cgggcaccat
     8401 cagaccctgc caggcgccgg cctcgaagcc cggcatcacc gtctcggcca gcgtcggcac
     8461 ctctggaaat agcgacatgc gcttggccgt ggtgacggcc agcagcttca tgcgcctgtc
     8521 cttgacgaag gccatgacgg agttgatggt gtcggtcatg aactggatct ggcccgccgc
     8581 cagatccacg tcggccggcg cgctgccacg gtagggcaca tgcgtggcct ctatgccctg
     8641 ggcgcggaca aactggtagg ccgccaggtg cgtcacattg ccgttgccgg ccgagccgta
     8701 ggacagcttg cccgggttgg ccttggcgta ggccacgaac tcctgcacgc tgttggccgg
     8761 cacggcgggg ttgaccacca gcgccagcgg caccaccgcc gtcagcgcca cgggcgccag
     8821 gtcgcgcagc acgtcgtact gcatgctctt gtacagcgcc gggctgagcg tgatggacga
     8881 ggtgttgtag agcagcgtat ggccgtcggc cgccgccttg ctgaccagcg cgccgccgat
     8941 gttgccgttg gcgccgggct tgttgtccac caccaccggc tggcccattt gctccgacag
     9001 cttttgcgcc agcaggcgcg ccaccatgtc ggtggggccg ccgggcggga agggcacaac
     9061 catggtgatg ggcttggccg gataggtctg cgccagcgcg ccgggtgcgg ccagtgccgc
     9121 cagtgcgccg caggcggcca gggccaggca ggcgcggcgg tggatggggt tcatgtctgt
     9181 ctcctcggtg tggtgtgtta ttgcgtgcgc ccggccagca gccgcgtggc ggtgtaggtc
     9241 atgacctgca cgccgccctg cttgaacacg tcgatgcgcg aggtgaccac ggcgcggccg
     9301 cttttcgacg tggggcgtat cccggtgacc tcgaccatgg cctcgatagt gtcgcccacc
     9361 agcacggggg ccaagatctt ctggtgaagc tccagcatgg ccaggcccgt gccctggatc
     9421 atggtctgca ggatgaagcc ctctatcagc gtgtaggtca gcgcgcccgg cacgggccgc
     9481 ccgcgcatgg cgccgccctc gtagccctcc tcgatgaaga tggcctccag catgccggtg
     9541 acgttgatga actgcaccag gtcgctctcg gtgaccgtgc ggcggaaggt tctgaagcgc
     9601 tggccgacct gcaggtcctg ccagacgaag ccctggccca ggcgcggagc ggcgtggtcc
     9661 atggtgtgcg tgccgttcat gtgtctgcat ccttgtgttg tggcgcaatc gcggcgcctt
     9721 gatcgatcag cgcctgcacc tgcgcggcat ccagccccac ctcggccagc agggcgcgtg
     9781 tgtgctcccc caggcgcggc gccatggtca cgggcgggcg ctggccgtca aaacgcacgg
     9841 ccacgccggg aaagcgcagc gtgcccatgg ccgcgtcctg cagttcctcg aagaagcccg
     9901 tggccagcag atgctcgtca gcgcgcagct cggccaggcg cgccaccggg gcggcgggaa
     9961 tctccagccg ctcgcaggtg gccagccaat aggccgtcgg ctggcgccgg acgtaggtgg
    10021 aggccagctc cagcagctcg gcgatatgcg ccgtgcggct ggcaatgtcg ctgaaacgcg
    10081 ggtcgtccgc atgctgcggc gcgcccacct cggcaaagaa gcgctgccag tgcagattgg
    10141 tgtagggcat catggccacc aggccatccg ccgtgtgata aggcctgcgc cagggcgcca
    10201 gcacgcgcgg gtagccgggc gcggagcgcg gcggctcgaa gtgctggccg tagaaatgct
    10261 cgaccaggtt gaagcccacc atggactcga acatcggcac ctcgacgaac gccccttcgc
    10321 ccgtgcgctc gcgctgccac agcgccgcca ggatggcatg cgccgccaca tggccgcagg
    10381 tcttgtcggc ggcgatggtg ggcaggtaac gcgcctcgcc gccctgctgc tccatcaggt
    10441 cagccaggcc cgacatgccc tggatcacat cgtcataggc cggccggccc gcgtacggcc
    10501 cgggcagaaa gcccagcagg ctggcgtaca ccagccgcgg atggcgcgcg cgcagcgtct
    10561 cgggatcgac gcccaggccg gccagcttct gcgggcgcat actgtgcatg aagacgtcgg
    10621 cggtcgctat cagcgcctgc agcgcctgac gcgcctcggg ccgcttcaga tccagcgcaa
    10681 tgcttttctt gctgcggttg gagcccagga acatggccgc catgccgggt tcgggcgccg
    10741 gcccggtgtg gcgcgtggag tcgcccccgg gcggctccac cttgatgacc tcggcgccat
    10801 agtcggccag cacctggctg gccagcgggc cgaagatgac gctggacaga tccagcaccc
    10861 gcagtccttg caagggcttc attcgcatgt ctcctgctgc tgtcttgctg gcgattattc
    10921 gggttcggcc cccatgcgtc taatatttat tggcgtccgt ccgatacctg ctgcgtatcg
    10981 aatggcgctc gtgagaccaa aaaactacgt gttttcccta gatacacaag cggcgccatg
    11041 caagcgagca ctcagcttat ggcgcgatga tgttccgatc atgaagattg ccctgctctc
    11101 cgacctccac gccaacctgc ccgccctcga tgcctgcctg gaacatgccc gcgcccaggg
    11161 ggcagaacaa tttgccctgc tgggcgacct ggtgggctac ggcgccgacc cgggcccggt
    11221 gctcgaccgc gtgatgcagc tcgtggccga tggcgcgctg tgcgtgcgcg gcaaccatga
    11281 cgatgccgca gtcgccccgc cgccccaggt ggagaacctg ggcgaccaga gcgcgcaatg
    11341 gacgcacccg cacctgtccg ccgaacagcg cgcctttctg gccggcctgc cgctgaccgc
    11401 gcgcatgggg ccggacgccc tgctggtgca tgccagcgcc gacggtcccg agcgctggca
    11461 ctacgtggcc gacagcaacg ccgccgagcg ttccatggcc gcggccacgc agatagaccc
    11521 ggccatccgc tatgtcttca gcggccatgt gcatgagcag gcgctgtact tcctcacgcc
    11581 cacggccaag ctgatgcgct ttagccccca gcccggcgtg cccgtgcccg tgccgccgca
    11641 ccggcaatgg ctggccatcg tgggctcctg cggccagccg cgcgatggcg acacgcgcgc
    11701 catgtatgcg ctgttcgacg ccggggccgc caccatcacc ttccagcgcg tgcgctacga
    11761 ccatatggcg gctgcggcgg cggtgcgcgc cagcggcctg ccggccttct ttgccgaccg
    11821 gctggaaaaa gggcgctagc gccatgaagc tgctcgagcc tggcaccgag gtcgatggct
    11881 tcgtggtgca cgagtgcata catgccggcg gcatggccca tatctaccat gcgggctacg
    11941 cgaatacggc gcgtgatccg ggctttccgc tggccatgaa gatcccgcgc atgacggccg
    12001 gcgatggcgc cgagaacatc gtcagcttcg aggtcgagct gaccatcttg cccaccctca
    12061 ccggccacca cgcgccgcgc ttcgtggccg cgggcgacct gatgcgtctg ccctatctgg
    12121 tcatggaata tgtggagggc cagacgctgc agcactggct ggacagccac cagcgcggcg
    12181 acgccgagca ggacgcccgc gacatcgccc gcatcggcag cgcgctggcc atcgccgcgc
    12241 acagcatcca ccagcagaat gtctgccacc tggacctgaa gccggccaac gtgctgctgc
    12301 gcccggacgg cagcgtggtg ctgctggact tcggcctgtc ctgccacgcg cattaccccg
    12361 acctgctggc tgaggagatg cgcaaggccg tgggctcgcc cacctggatc gcgcccgagc
    12421 aggtggtggg cgtgcgcggt gatctgcgca gcgacatctt cgccatcggc gtgatgctgt
    12481 acgagatggc cacgggcgag ctgccgtttg gctcgcccac caccgacgcc ggcctgcgcc
    12541 agcgcctgtg gatgacgccc gccccgccgc gccagcaccg cccggatctg cccgagtggc
    12601 tgcaagaggt catcctgcgc tgcctggagc ccgaggccgc gcagcgctac ccctcggccg
    12661 cacacctggc cttcgacctg gcgaacccgg agcaggtgcc actcaccgag cgcgggcggc
    12721 gcctgcgcgg gcccggcctg cgcgtgcact tcaaacgctg gatcaaggcc gccggcatgc
    12781 actaccagcc cagcccgcta cccacgcgcc agatcgagga agtgcccatc ctcatggtcg
    12841 ccgtgccgca cgacgacgtg accgacgcca cgctgtactc gctgcgcgag gcggcggcgc
    12901 gctcgctggg catacgcccg ggcgcgcgcc tggcctgcgt gtcggtgatc tcgccatcgg
    12961 ccagcagcgc gtcggacagc gagcgcagcg aaaccacgct gcgccgccag cacctggcgc
    13021 ggctcaagca atgggcgacg gggctggatc tgtcgcgcca cagcgccagc taccatgtgc
    13081 tggagtccag cgacgtggcc caggccctgg tgcgctacgc cgaggcgaac cgcgtcagca
    13141 tgatgatcct gggcgcggcc acgcatggcc tgcaactgca gcgcttcgta gccaccgtac
    13201 ccatgcgcgt ggcgcgcgac gcgccctgca ccgtcatcct ggtcaagcag cagctgccgt
    13261 tcgagcacct gtagccgcca tcagcaacca cccatcacgc ggcactcggg caccggccgg
    13321 ccttgtgcat cgcgccactg caggggtgcg ccctgcgccc cgggcaccgg cggcggcgca
    13381 gcgccaggtg tagccgccgg ctgctgcggg tcatgcagca ccgtggagct gcccaccccc
    13441 acacccacgc gggcattgcc catgggcacc gatgccccca ccccggcacc acccgtgacc
    13501 tggccgcgct gattcacccc cacgccgacg ccaagcggcc cgacgccggt ggacacgccc
    13561 gccgacacac cgccggaacc cagccccacg ccaatgctga cggggcccac tggcaccccc
    13621 acgcccacat gcgccgatgg gctggcgcat gcggccagga atgccgccat ggcgagcgcg
    13681 gccgcgccgc gccagcctgt ctcgttgatt tgccttgcca tgccccatgg tagccaaccc
    13741 ggcgcggccg gcacgccaca gcggcgcgcg acaatgcacg ccatgaccgc aacgcccgca
    13801 gacaccgccc cgccccaccc ctacgccagc ctcacgccgg acctggtgct ggacgccctg
    13861 gccagcgtcg gcctgtacgg cgacggccgg ctgatggccc tgggctcgta tgagaaccgc
    13921 gtctaccagg tggcgctaga ggatggcagc cgcgtggtcg tcaagttcta ccgccccgga
    13981 cgctggagcc aggcgcagat cgaggaggag catgcattcg cgctggagct ggccgccgcc
    14041 gaggtgcccg tggtcgcgcc cctgatgctg cagggccgca gcctgcaccg gcacgccggc
    14101 tttggctttg ccgtcagccc ctggcgcggc gggcgcatgc cggagctgga tgacttcgag
    14161 gtgctggaat ggctggggcg ctacctggcg cgcatccaca acgtaggggc ccagcgcccg
    14221 ttcgaacaac gcccgcggct ggatgcgcaa agctttggcc aggagccgcg cgactggctg
    14281 ctcagccagc aaatcatccc cctggaccag caatcggcct ggcacgcggc ctgcgatgaa
    14341 gctcttgaat tgatagctgt tgacgcttat ccatcaagcg ctacaggcca atttggcttg
    14401 aaagatgcaa acgccatccg cctgcacggc gactgccatc ccggcaatgt gctgtggaca
    14461 cccgtggacg acgcaggcca gggcggcccg catttcgtgg acctggatga cgcgcgcatg
    14521 ggcccggcgg tgcaggacct gtggatgctg ctgtcgggcg accggcgcca gcgcgcgctg
    14581 cagctgtcca cgctgctgga cggctacgag cagttgcgcg ccttcgaccg gcgcgagctg
    14641 gcgctcatcg agccgctgcg cacgctgcgc ctgatccact acagcgcctg gctggcgcgg
    14701 cgctggagcg acccgatctt ccccatcaac ttcccgtggt tcggcagcag cgactactgg
    14761 cgcggccaag tgggcatgct gcgcgagcag atcgaggcga tgcgcgagga gccgctgacc
    14821 gcctgaaaag cacaggcggc caggcccggc atcattcagc cttgacgccg gagatcttca
    14881 ccagcttctg gatgacggga tcgacggcgt caaagtcctt gtggaactgc gccgcgccgc
    14941 ccggcaccga ctccaggcca aagacctgga agcgcgcctt catggccgtg gagtggatga
    15001 tctccaccga ctccttctcc agccgctgca gtatgggctt gggcgtgccc ttgggcgcca
    15061 gcagggtgaa ccaggccacc gtgttgaact ccgggtcggc aaagccctgc tcggccatgg
    15121 tgggcacgtc gggcagctcg gccaggcgct tggggccata gatggcgatg gggcgcacgc
    15181 ggttgtcctt gagcacgccc tgggccgcgg caatcgtccc cagaccccac gagaccacgc
    15241 cgcccgccag atcctggata tgcggcgact cgctcttgta ggccacatgc gtcatgtcca
    15301 gcttgcgcgt gtcgctcagg taggcgctca tcaaatgccc ggccgaaccc tgtccgtatg
    15361 acccgtagtt gaccttgccc ttgttggcct gcgcccaggc catgaactcc ttcatgttct
    15421 tgaccggcac attggcgttg accatcatca ccaggctggt ggaggccagc tgggtgatga
    15481 actcgaagtc cttgcggatg tcgtagggca ccttggcaaa gatgtggtgg gcatagaaca
    15541 cgggcgtgga ataggccacc agcagcgtat agccatccgg cgcggcgcgc gccacggcct
    15601 gcgtgccgag catgcccgag gcccccgtct tgttgtccac caccaccgcc tggcccaggc
    15661 gcttgcccaa ctggtcggcc agcacccgcg ccaccacatc cgtaccgccg ccggcgccgg
    15721 cgggcaccac cagggtgatg gggcggttgg ggtagtcctt ggcggcgctg gcggtctggg
    15781 cctgcgccca gcctgccgcg cccagggcca gggccatggt ggccagtacg gtcttgatcc
    15841 tcatgcatcg tctcccggtg gtcaaaggga aggatgctgc gagaacaacc ggccagtcgc
    15901 caatagggga tacctgtaaa acaacgcgat cagcagcagc aacggcacgc tggaagcgcc
    15961 aaacccctgc cttgcgctcg tcgcgcaccc ggccaaaacc tcaaccgatc aagcctgaac
    16021 gctttggcag caagccttca cagcgatccc aaaagggcat gcatcgagcc tgcggcgcgc
    16081 cccagccaac agaatgggcg ctttccacgg tatttcgtta gcaaccatat tggtatgacc
    16141 aatactgaat ttaccctgat tgtttatgaa tttaggtatt tacccggtat gcattgataa
    16201 atgcgcacgc cgaaaatagc cacgcctcgg atctcatgcg tattggtagt accaatttca
    16261 ctttcacgcc atggcacgag gcatgccacc ctccccgttg accccgcccc ccagcggcag
    16321 gccagcggga agccactgaa accgcaggcc ccgcggcccg cacaacgcat ttcgcacaac
    16381 ggccatgcaa accctctggc aacagaacta cgaccccgcc gggaatatct ggatctcggc
    16441 cctggtcgcg ctcattccca tcatcttctt cttccttgcc ctgaccaagc tgcgcctcaa
    16501 gggctaccag gccggcacca tcacggtgtt gctggccctg ggcgtggcgc tgctgttcta
    16561 caagatgccg gtgagcgcgg cgctggcctc ggccgtgtac ggcttcttct acggcctgtg
    16621 gcccatcgcc tggatcatcg tggcagccgt tttcctctac aagatctcgg tcaagacggg
    16681 gcagttcgac gtcatccgct cgtcgattct gtcggtcacg cccgaccagc ggctgcagct
    16741 gatcctggtg ggcttttgct tcggcgcctt cctggagggt gcggcgggct ttggtgcgcc
    16801 cgtggccatc acggccgcgc tgctggtggg tctgggcttc aagcccttgt atgcggccgg
    16861 cctgtgcctg atcggcaaca cggcgcccgt ggccttcggc gccatgggca ttcccatcat
    16921 cgtggccggc caggtctccg gggtagacgc cttcgccatc ggacagatgg cggggcgcca
    16981 gctgccgttc atgaccgtga tcgtgctgtt ctggctcatg gccatcatgg acggctggcg
    17041 cggcgtcaaa gaaacctggc ccgccgtgct ggtcggcggc ggctcgtttg cggtgatgca
    17101 atttctgacc gccaactaca tcggtcccga gttgcccgac atcacctcgt ccatcgtctc
    17161 gctggtggcg ctgacggcct tcctcaaggt ctggcagccc aagcgcatct tccgcttcga
    17221 gaccgagagc caacccggca ccaagcccgg cgccagcgcc agcgcccccg ccacggcgca
    17281 ccacccgctg accgccgtgg ccatcgtcaa ggcctggtcg ccgttcatca tcctcacggc
    17341 catggtgacg ctgtggagcc tcaagccctt caaggccctg ttcgccgccg gcggcccgct
    17401 ggcatcgacc atcatcaaca ttcccgtgcc catgttgcac aacctggtag agaaggcgcc
    17461 gcccgtggtg gccgccgcca caccctatgg cgcggtctac accttcaact ggctgctggc
    17521 cacgggcacg gccatcctga tcgccgccat catcaccatt gccttcacgc gcctgtcgcc
    17581 cgccaaggcc gtggccacgc tgggcgagac cttcaaggag ctgctcgtac cgatctactc
    17641 catcggcatg gtgctggcgt tcgccttcgt ggccaactac tctgggctgt cggccacgct
    17701 ggccctggcg ttggcgcaca ccgggcacgc cttcacgttc ttctcaccct tcctgggctg
    17761 gataggcgtg ttcctgacgg gatcggacac ctcggcgaac gcgctctttg gcgcgctgca
    17821 ggcgaccacc gcgcagcagc tgggcctgcc ccaggtgctc accgtggcgg ccaacaccac
    17881 cggcggcgtg accggcaaaa tgatctcgcc gcagtccatc gccatcgcct gcgcggccgt
    17941 gggactggcg ggcaaggagt cggatctgtt ccgcttcacc gtcaagcaca gcctgatctt
    18001 cgccgtcatc atcggcatca tcacaacgct gcaggcctat gtatttacgt ggatgattcc
    18061 gggccactga accacggttg aacccccaac tggagagccc ccatgcgcct ggccgatcac
    18121 gttgtcgaga aactgcttgc cctggtgcag gcccgcggcc tgcagcccgg gcagcgcctg
    18181 cccgcggagc ggcaactggc cgaggaactg ggggtgtcgc gcacctcggt gcgcgaagcc
    18241 atacagaagc tgaccagcca gggcgtgctg tcggcgcggc gcggcgacgg cacctacctg
    18301 cagtcgcgcg cgacggccga atggctgcag gagggcatgc agccgctggc cggcctgatc
    18361 gacgccgacc cgcactaccg ctacgacgtg ctggaggccc ggcaggcgct ggaaagcagc
    18421 gccgcctggc tggccgcgca gcgcgccacc gcccaggaca aggcgcgcat acagcactgc
    18481 ttcgaggtga tgcttgacca tcagcaaaac ggccacgccg aattggccgc gcgtgccgac
    18541 gcacagttcc acctggccat tgccgaggcc tcgcacaacc tggtgctggt tcaggtgacg
    18601 cacagcctgt tcaccacagt gctgtccacg gtggcgcgca accgtcacga catgttccac
    18661 ctcagcgcgc ccgtgaccgt gcaggcgctg acggaacaac accaggcgct gatgcaggcc
    18721 atcctggacg gcgacccgca gcgtgcacgc caatgcatcg atgaacatct ggagcatgtg
    18781 cgcgtcacca tccagcgtct ggacgaagac cgcgcacggc aggaacgttc cacacgcctg
    18841 ccgccggatc tggtaccacc gctgctaccc gaaacgtccc cacacacctg aacctctcgc
    18901 catgatcatt tcctccagcg ccgattaccg cgcagcggca caaaaattcc tgccgccctt
    18961 cctgttccac tacatcgacg gcggcgccta tgccgagcag acgctgcgcc gcaacgtgga
    19021 cgacctggcc tccgtggcgc tgcgccagcg cgtgctcaag gacatgagcc agctcgacac
    19081 cggcatcgag ctgttcggcg agaaactcgc cattcccgtg gcgctgtcgc ccgtgggcct
    19141 gaccggcatg taccgccgcc ggggggaggt gcaggccgcg cgcgcggccg acgcacacgg
    19201 catcccgttc accatgtcca gcgtctcggt ctgccccatc gaggaggtcg tgcccaagat
    19261 caagcgcccc atgtggttcc agctctacgt cctgaaagac cggggcttca tgcaaaacgc
    19321 gctggagcgc gcgcaggccg ccggctgctc caccctggtg ttcaccgtgg acatgcccgt
    19381 gcccggcgcg cgctaccgcg acgcccactc cggcatgagc ggccccaacg ccccgctgcg
    19441 ccgctactgg caggccatga cgcacc
//
LOCUS       DAOMTQ010000044        19161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_361_length_19161_cov_27.707003, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000044 DAOMTQ010000000
VERSION     DAOMTQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_19161_cov_27.707003"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1961
                     /locus_tag="PK518_06310"
     CDS             <1..1961
                     /locus_tag="PK518_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM48562.1"
                     /translation="PALWWLRRRVRRLLALPLRRLTQAVDELAQGHEPAPIRLARTDE
                     LGKLATAFDAMAADLAQQRQWQVAHAQELQTEVDERRHVMQRLQQERARLLALLGAMH
                     QGILFVDTDQHVSYCNAMFRELWAMPGGQAIVGLPMEQVLQACRGQLLAAARLDEQVC
                     QSLAGEPAAAPLELELASGRILMLAIHRVHDTQNASMGHLWVCEDVTQQRQTAAQLIT
                     LAERDALTGLYNRHCFEQALERFFRESACHAQPAAVLFFDLDEFKYVNDRFGHGAGDM
                     VLLRVATAVKALVRETDLLSRLGGDEFAIFMPQATLHSAQLLAERVVRAVAQAALHIE
                     GEGHTLRLTSSLGIAHFPTHAASAQELVAHADTAMYQAKHLGKNRWSIYRAESDRAQA
                     MVTRMAWNERIDRALAGGLLRLHFQGVYHAQERRLAHLEALVRMVDEDNPEQLIAPGQ
                     FIGHAEKSGKILDIDRWVLRESIRLLAAHPRLPALALNISGRSLDDPDLPGFITEQLA
                     AQGVAPERLLVELTETAAISDLGDAQRFIDALQRAGCTLCLDDFGTGFASFAYLKHLQ
                     ARVLKIDGLFVRNLPREHDNQVFVRSIIEVAHGMGKLAVAEFVEDEQTLQLLQGMGVD
                     MVQGYHLDKPRADHPGLHGDGRAGILPA"
     gene            2120..3649
                     /locus_tag="PK518_06315"
     CDS             2120..3649
                     /locus_tag="PK518_06315"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRM48563.1"
                     /translation="MTDPRPQCPVTHLTTDFGAPVASNRDSLTAGSRGPLLAQDVWLN
                     EKLANFVREVIPERRMHAKGSGAFGTFTVTKDITRYTRAKIFEKVGKQTEMFARFTTV
                     AGERGAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFIRDPRQFPDLNKAVKRDPKT
                     NMRSATNNWDYWTLLPEALHQITIVMSERGIPASYRHMHGFGSHTYSFWNAAGERFWV
                     KFHFKTQQGIKNLTDAEAEALIGKDRESHQRDLFDAIARGDFPKWTMYVQVMPEADAE
                     KVPYHPFDLTKVWPHADYPLIEVGEFALNKNPENFFLDVEQSAFSPSNLVPGIGVSPD
                     RMLQARLFNYADAQRYRLGVNHQQIPVNKARCPVHSNHRDGAGRVDANYGGAPHYEPN
                     SFGQWQAQPEFAEPPLRISGDAQHWSFHEDDSNYFQQPGNLFRLMTDAQKQALFGNTG
                     RGMGDAPEFVKYRHIRNCHAADPAYGAGVAKALGLDLQKALASQKDDPMFGNPLVALP
                     V"
     gene            complement(3653..6220)
                     /locus_tag="PK518_06320"
     CDS             complement(3653..6220)
                     /locus_tag="PK518_06320"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM48564.1"
                     /translation="MPDQPTPDEPLQGLSSQQAAERLASDGPNQLPGSAPKSNLAIVR
                     EVLTEPMFLMLLAAGGTYLALGDRGEALFLLGSVLVVIGITLTQERKTQRALESLREL
                     SAPRALVLRDGVERRIAGREVVRGDLLVLREGDRIAADARLLNGQLEVDESLLTGESV
                     PVARLPLSDGALAEEAHDARADSLHASTVVTRGVGLARVLATAGDTAVGRIGAGLAAT
                     ETPRSPLQLRSRRLVRALGALALLLATAQVLLGWWWNGRPLLDSLLAGIALAMAILPE
                     EIPVILTVFLALGAWRIARQQVLTRRITAVETLGAITVLAVDKTGTLTQNRMAVAELA
                     TPAQTWQAAGANALPEPFHRLVEFAMLATPTDPFDPMEKAIARFGHEWLKGTEHVQDG
                     REPEFEYPLSSDILAMTRVFASGQPHMYQLATKGAPEAVADLCHLDEAGRNALRAQVE
                     SLAERGLRVLGVARGHWTGAAQGALWPASQHDFDFEFLGLLALADPPRPEVPDALAQC
                     RAAGVRVVMMTGDHPATARAIARQVGLSERPEVITGDELQGLDDSALRARLHQVDICA
                     RLKPAHKLRLVRLLRDDGAVVAMTGDGVNDAPALKAAHVGIAMGERGTDVAREAAALV
                     LLQDSFAHIVAAIRQGRRIDDNVRKATRFVFAVHVPIIALALAPTLLHWPALLLPVHI
                     VLMELLIDPACSIVFEAEPEAPDIMQRPPRAVADSPFALRALWPALLQGAGVAALLLG
                     AQAWLQAQGWSMAEGRTVVFGALVLCVLLLILAQRDGRVPGRGGAPNPWLLPMAAAVG
                     LLLAALLVIPGLGPLMGLGNPGLPGLAAGAAVLLPCLAWLELLRQLRLRLARQRRAA"
     gene            6295..6777
                     /locus_tag="PK518_06325"
     CDS             6295..6777
                     /locus_tag="PK518_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48565.1"
                     /translation="MTKTEHPAYFEQQHERLDAQLHAHLLDVVGGDFAGALQRLQQWR
                     RDLDRHTEIENARLLPHVPEGARWAARVYLLEHERIALLADEYQQRVQAVINHPPQDE
                     QARRRAVLVLLDAAHALRHVLEHHNEREHIALAQELPLELQKAAWADEPASQVRTPGS
                     "
     gene            complement(6758..7222)
                     /locus_tag="PK518_06330"
     CDS             complement(6758..7222)
                     /locus_tag="PK518_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48566.1"
                     /translation="MLALFLPQPRSLSMKKSIASALLLACAATAIHAEELFVSIHSAS
                     PMAQGAGLVLAGQALEQKAPVRVLLCDAAGDIALANTDAAKAQPILKPRNVTPQQMLQ
                     GVIKAGAKVEVCALYLPNTGKQPSDLLPGVSPAKPAEVATHLLKPTVKTLAF"
     gene            complement(7355..7654)
                     /locus_tag="PK518_06335"
     CDS             complement(7355..7654)
                     /locus_tag="PK518_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48567.1"
                     /translation="MRTTLLLAAIAMLASAPVLAGEREDRHERQQRTEQTAPNGFDHG
                     MQPVAHNAGPGERAHGWRYFSDPATYRAVVISPDGDYYLSRGKGLRWVAGAQTQD"
     gene            complement(7754..8725)
                     /locus_tag="PK518_06340"
     CDS             complement(7754..8725)
                     /locus_tag="PK518_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRM48568.1"
                     /translation="MPKTCSERAAPETTRVVLHGPTMGTRWSATCELAPQIDARALQR
                     DLAATVERVDAQMSPWRSDSCLVQLNQAPVGAWFALTTEMLQILACALDIGRLSQGAF
                     DIGVGALVDAWGFGAARSAPDASAIRAARALAHGPAHETLELDLHAGRARKLAPLQID
                     LCGIAKGYAVDRMAEVLQHHGVAQALAALDGELRAIGGQADGRPWAVAIESPQTGLRR
                     AHGVLALQDLAVATSGDYRHFFDVNGQRLAHSMDARSGTPVRGDVASVTVLAANCMLA
                     DAWATALLVAGAAQGLALAQRHGLEALWLLRRGGALVELGLGRFGGA"
     gene            complement(8706..10913)
                     /locus_tag="PK518_06345"
     CDS             complement(8706..10913)
                     /locus_tag="PK518_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRM48569.1"
                     /translation="MGWRTIHRWLGLTLGALALVLGLSGALLAIDPVHQAWQAPAAAP
                     DLPVATLAERVQAHMPGVEEIRHLPTGRIAALGFDGEQPQALYVDPASGALLGPYQPS
                     ALPRWVKNLHRALLLGDAGRWGAAAAALALALLSISGAVLLLRRMGGWRQLTGRVRGT
                     LAQRLHVLSGRVLLAFLALTSVTALTMSTSTLGLVQLEAGPEPEVSSGAGAADLPAGR
                     IPLLQELRTSQLRRLNLPDAADTSDTWRVTTSSGQGWLDRHSGALLAWQDATGAQWLY
                     DWAMLLHTGESAWPWAVVLGVSGASVLLFWATGLLIWWQGHRGAVHIAHNSPLAQADA
                     LIFVASEGGSTWGFAQALHAALLQAGRRVHTTGLENFQTTAATQQVFILAATYGDGQA
                     PAHAAQAQARIAAQPVRPLPVTVLGFGDRQYPAFCSFAKALDAQLRTRGWPTLLPLEC
                     IHQQSGQEFARWGKTLAQALGQVLVLDYVPQLPPTTALTLRSRQDYPGAQDGRPAAIL
                     RFDWPRPGWFERLRGHGLAPFAPGDLVAILAPGSSVPRYYSLASGSADGFLEICVRQW
                     PGGLCSTHLLGLQPGAQVPAFIRPNPGFALQGRQPSVLLIGAGTGVAPLAGFIRRNDR
                     RTPMHLYLGARDPAHDHYFGADIKRWLTERRVASVHTAFSRVPGGGGYVQDALRRDAQ
                     RLRALLAGGAMVRVCGSRPMASAVTEVLDTIAATLGLSVRELKATGRYAEDLF"
     gene            complement(10923..11399)
                     /locus_tag="PK518_06350"
     CDS             complement(10923..11399)
                     /locus_tag="PK518_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HRM48570.1"
                     /translation="MSKPLLGTLAAACALALPTLAHSRPVTISAQLKNYGGDGAYLAI
                     YLTDAKGAYAGTLWVAGGKAKYYKHLASWNRLSAGDARRLNGVTGASVGAGRTLKVTA
                     DLADALIDAGYEIHVDVAVENMRDSPSELRVPLSTQNAGKPQAGKQYIQSLSFQLQ"
     assembly_gap    11414..11513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11543..11938)
                     /locus_tag="PK518_06355"
     CDS             complement(11543..11938)
                     /locus_tag="PK518_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRM48571.1"
                     /translation="MPRPHTLVRHGLLTLCLGLASLPAMADDDCNAPSQRWQTREAVR
                     QMAAQRGWDLQRIKIDDGCYEVRGQDASGRRFKAKIDPETLAVLKFKHRDGHRDQERR
                     RTQEHGAPPAQGSTAPVFTPGGSPRGQID"
     gene            complement(11973..13361)
                     /locus_tag="PK518_06360"
     CDS             complement(11973..13361)
                     /locus_tag="PK518_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRM48572.1"
                     /translation="MRRTLSPATALALWLALITLAWWAVAPAQLGIDPLVNAQGEATG
                     WWQLRRHAIYLTGLWCMGLMGLIMVLALRLPLIERPLGGMDQVYRLHKWAGMGAGITA
                     LLHWGVKEGGGLIKATWGREGKPARDAVLPWFTDARDLAKDLGEWAFYALLVLIALTL
                     LQRLLAYRPWRLTHRAMPLLYLGFVFHALALTPLAYWALPLGLLQAGLLTLGSAAALW
                     SLAGGIGLRRRHGAHIHSVQLLGQGPDAPLEVVCALPDSWPGHCPGQFAFVRFDRAEG
                     AHPFTIASAPGALGENARGEALLRLVIKPLGDYTRTLARRLQAGQGVDIEGPYGRFDG
                     RGSRARQQVWVAGGVGVTPFIALLEARQPGLATADARLQPVQMHYCTRDAARDALLGR
                     LQTLCAQAEPAVTLTVHDDARGQRLTPRTLEALGGPLDIWFCGPQGLGDALGAHASTA
                     PWRLFRESFAMR"
     gene            complement(13404..13769)
                     /locus_tag="PK518_06365"
     CDS             complement(13404..13769)
                     /locus_tag="PK518_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRM48573.1"
                     /translation="MSFLAVFNLKEFTMHSTTTRRCLIATVIAASAIGLGLAQAQTSP
                     PAATASPAAASAPAQKLSIRDIAERLEAAGYRDILEIDYEHGRYEAKALNAQGAPVKL
                     YVNAQTGAVERTRTRKQHD"
     gene            13867..14187
                     /locus_tag="PK518_06370"
     CDS             13867..14187
                     /locus_tag="PK518_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRM48574.1"
                     /translation="MPALTRLLALLLTACALSLPAAAHDHDEHERARQALEQGRVLPL
                     TSVIAKVEREHPGQVVKIEFEEDDGRYIYEIRLLQRDGRMAKLKVDAVDGRVLKIKRK
                     ERKE"
     gene            14196..14870
                     /locus_tag="PK518_06375"
     CDS             14196..14870
                     /locus_tag="PK518_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM48575.1"
                     /translation="MRILVVEDEPTLQAQLVQAIAGAGHTVEAAADGREAQYLGEVED
                     FDAVVLDLGLPLLDGLSVLRHWRAAGRRMPVLILTARSSWQEKVAGMDAGADDYLAKP
                     FHMEELLARLRALLRRLGEHASAEWQCGAISLDTRAARVLVAGQPLTLTSHEFKLLAQ
                     LMQRKGEVLSRTELAEHLYPQDSERDSNTIEVFVGRLRKKLPEGSIETVRGLGYRLTD
                     GDAAAP"
     gene            14867..16225
                     /locus_tag="PK518_06380"
     CDS             14867..16225
                     /locus_tag="PK518_06380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM48576.1"
                     /translation="MKHSLRLRLLAGMLVWMLLTIAAAGWGLRALLQGHVEQQLAVQL
                     VMDLDHLSGSIDWAPDGRLDVENQPDARLAQPLSGLYWQIDRLGAQPQVALARSRSLW
                     DQTLRLPAAPSAYPAQGHSLLRMADARGRALLVVARTVQLPDEGAPPLRLAVAADEAL
                     IAEPMARFTRLLLIALGALALALALAVVLQLQLALRPLRLLRERLAAVRAGQAARLEG
                     RFAQELEPLVAEFNHVLAENADMVQRARTQAGNLAHAVHTPLTILANAADGQATPLAR
                     LVQEQVASARRQVDYHLARARAAAAVRATGLATPVLEPLRALLRTMARLHGARGLSFE
                     LADGAQDLAFRGETQDLYEMLGNLMDNAGKWARTRVVVDVRREGQQLCFTVDDDGPGI
                     PEEQRQRMFERGVQLDERRTGSGLGLDIVRALADSYGGSVQALASPLGGARLVLRLPA
                     AA"
     gene            complement(16382..>19161)
                     /locus_tag="PK518_06385"
     CDS             complement(16382..>19161)
                     /locus_tag="PK518_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRM48577.1"
                     /translation="KPDVDLIEGLSPAISIEQKATSHNPRSTVGTVTEIHDYLRLLYA
                     RAGTPHCPDHGLPLAAQTVSQMVDAVLQLPEDTKLMLLAPVARQKKGEFVELFEQMQA
                     QGYVRFRVDGRIYEVDDLPELKKTEKHDIDVVVDRLKVRADAQQRLAESVEAVLRIGG
                     AEGAGRVLALEMDSGREHLFSAKFACPVCSYSLSELEPRLFSFNSPSGACPACDGLGQ
                     QELFDPARVVAFPSLSLASGAIKGWDRRNGYYFAMLESLAAHYGFDVDTPFEQLPEQV
                     RQVVLHGSGEEDIAFSYLLDSGPKKGQPIVKKHPFEGILPNMARRWRETDSSVVREDL
                     ARYRQTQPCAECGGARLRREARHVLVGEAEAARAIYAVSDMTLADAQAWFGALRLSGA
                     KAEIADKIVREIGARLGFLNDVGLSYLSLSRSAETLSGGEAQRIRLASQIGSGLTGVM
                     YVLDEPSIGLHQRDNDRLIATLTRLRDLGNSVIVVEHDEDMIRAADHCVDMGPGAGVH
                     GGRVMAQGSYAELCANPASLTGQYLSGARTLPVPARRTPWLPVLQQGAAPEDENKPKS
                     RFPETAASQRKRARLAEHRATQGQLQAVRVLGATGNNLKGVNAEFPVGLLTCVTGVSG
                     SGKSTLVNDTLHRAAARLIHRAHEEAAPHDAIEGLEYFDKVINVDQSPIGRTPRSNPA
                     TYTGLFTPIRELMAETNTAKERGYGPGRFSFNVSQSAGGGRCEACQGDGVVKVEMHFL
                     PDVYVPCDICHGQRYNRETLEVQWKGKNIAQVLELTVEDAYAFFKDVPTIARKLQTLL
                     DVGLSYIRLGQSATTLSGGEAQRVKLAQELSRRDTGRTLYILDEPTTGLHFADIDLLL
                     RVLLQLRDAGNTIVVIEHNLDVIKTADWLIDMGPEGGAGGGSVVAVGMPEEVAAQEGS
                     HTGRYLRRYLS"
BASE COUNT         2926 a         6564 c         6685 g         2886 t
ORIGIN      
        1 tgccggccct gtggtggctg cgccggcgcg tgcgccgctt gctggccctg cccttgcggc
       61 gcctgaccca ggcggtcgat gagctagcgc aggggcatga gccggccccc atccgcctgg
      121 ccaggactga cgagctgggc aagttggcca ccgccttcga tgccatggcg gccgacctgg
      181 cgcagcagcg gcaatggcaa gtggcccatg cccaggagct gcagaccgag gtcgatgagc
      241 gccgccatgt catgcagcgc ctgcagcagg agcgcgcgcg gctgctggcg ctgctgggcg
      301 ccatgcacca gggcatcctg ttcgtggata cggatcagca tgtcagctac tgcaatgcca
      361 tgttccgcga gctgtgggcc atgcccggag ggcaggccat cgtcggcctg ccgatggagc
      421 aggtgctgca ggcatgccgg ggccagttgc tggccgccgc acggctggac gagcaggtgt
      481 gccagagcct ggccggcgag ccggcggcgg ccccactgga gctggagctg gcgagcggcc
      541 gcatcctgat gctggccatc caccgggtgc atgacacgca aaacgcttcc atgggccatc
      601 tctgggtgtg cgaggacgtg acgcagcagc gccagacggc cgcgcagctc atcaccctgg
      661 ccgagcgcga tgcgctcacc ggcctgtaca accggcattg cttcgagcag gcgctggagc
      721 gcttcttccg tgagtcggca tgccatgcac agccggcggc ggtgctgttt ttcgacctgg
      781 atgagttcaa gtacgtgaat gaccgctttg gccatggcgc tggcgacatg gtgctgctgc
      841 gcgtggcgac ggcggtcaag gccctggtgc gcgagaccga tctgctcagc cgcctggggg
      901 gcgacgagtt cgccatcttc atgccccagg ccacgctgca cagcgcccag ctgctggccg
      961 agcgcgtggt gcgcgccgtg gcccaggcgg cgctgcatat cgagggcgaa ggccataccc
     1021 tgcgcctgac gagcagcctg ggcatcgccc atttccccac ccatgccgcc agtgcccagg
     1081 agctggtggc ccatgccgac accgccatgt accaggccaa gcacctgggc aagaaccgct
     1141 ggagcatcta ccgcgccgag agcgaccgtg cccaggccat ggtcacgcgc atggcctgga
     1201 acgagcgcat tgaccgcgcc ctggcgggcg gcctgctgcg cctgcatttc cagggcgtgt
     1261 accacgcgca agagcgtcga ctggcgcatc tggaggcgct ggtgcgcatg gttgatgagg
     1321 acaaccccga gcagctcatt gcccccggcc agttcatagg ccatgcggag aagagcggca
     1381 agatcctgga catagaccgc tgggtgctgc gcgagagcat ccgcctgctg gcggcgcacc
     1441 cgcggctgcc ggcgctggcg ctgaacatct caggccgctc gctcgacgac ccggatctgc
     1501 cgggcttcat cacggagcaa ctcgcagcgc agggcgtggc gcccgagcgc ctgctggtgg
     1561 agctgaccga gacggcggcc atttccgacc tgggcgacgc ccagcgcttc atcgacgccc
     1621 tgcagcgcgc tggctgcacc ctgtgcctgg acgacttcgg taccggcttt gcgtcgtttg
     1681 cctacctcaa gcacctgcag gcccgggtgc tgaagatcga cggcctgttc gtgcgcaacc
     1741 tgccgcgcga gcatgacaac caggtgttcg tgcgctccat catcgaggtc gcccatggca
     1801 tgggcaagct ggccgtggcc gagttcgtcg aagacgagca gaccctgcag ctgctgcagg
     1861 gcatgggcgt ggacatggtg cagggctacc atctggacaa gccccgtgcc gatcaccccg
     1921 gcttgcacgg cgatgggcgc gccggcatcc tgccggcgtg agggcgctgg cgcctgcggc
     1981 atggggccag ccccgcgcca gagtcgctct atctgtgtaa taaaaagtat taaattttca
     2041 aataaatcta aaaattctat tattcaaccg tggcacttgc ccattgatcc ccttccccac
     2101 cgcaacccag gagatagcca tgaccgaccc tcgcccccaa tgccccgtca cccacctgac
     2161 gaccgacttt ggcgccccgg tggccagcaa ccgcgacagc ctgacggccg gcagccgcgg
     2221 cccgctgctg gcgcaggacg tctggctgaa tgaaaaactg gccaacttcg tgcgcgaggt
     2281 catccccgag cggcgcatgc atgccaaggg ctcgggcgcg tttggcacct tcaccgtgac
     2341 gaaggacatc acccggtaca cccgcgccaa gatcttcgag aaggtcggca agcagaccga
     2401 gatgttcgcc cgcttcacca ccgtggccgg cgagcgcggc gcggccgacg ccgagcgcga
     2461 catccgcggc tttgccctga agttctacac cgaggagggc aactgggaca tggtgggcaa
     2521 caacacgccg gtgttcttca tccgcgaccc gcgccagttc cccgacctga acaaggccgt
     2581 caagcgcgat ccgaaaacca atatgcgcag cgccaccaac aactgggact actggacgct
     2641 gctgcccgaa gcgctgcacc agatcaccat cgtcatgagc gagcgcggca ttccggcgag
     2701 ctaccgccac atgcacggct ttggctcgca cacctacagc ttctggaacg cggccggcga
     2761 gcgcttctgg gtcaagttcc acttcaagac acagcagggc atcaagaatt tgaccgacgc
     2821 cgaggccgag gcgctgatcg gcaaggaccg cgaaagccac cagcgcgacc tgttcgacgc
     2881 cattgcccgc ggcgacttcc ccaagtggac gatgtacgtg caggtcatgc ccgaggcgga
     2941 cgcagagaag gtgccctacc accccttcga cctgaccaag gtctggccgc acgccgacta
     3001 cccgctgatc gaggtcggcg agtttgcgct gaacaagaac cccgagaact tcttcctgga
     3061 cgtggagcag tcggcctttt cgcccagcaa cctggtgccc ggcatcggcg tctcgcccga
     3121 ccgcatgctg caggcgcgcc tgttcaacta cgccgacgcg cagcgctacc gtctgggcgt
     3181 gaaccaccag cagattccgg tgaacaaggc acgctgcccc gtgcacagca accaccgcga
     3241 cggcgccggc cgggtggacg ccaactacgg cggcgcgccg cattacgagc ccaacagctt
     3301 tggccagtgg caggcgcagc ctgaatttgc cgagccgcct ttgcgcatca gcggcgacgc
     3361 ccagcattgg agcttccatg aggacgacag caactacttc cagcagccgg gcaatctgtt
     3421 ccgcctgatg acggatgcgc aaaagcaggc gctgttcggc aacaccggcc ggggtatggg
     3481 cgatgcgccc gagttcgtca agtaccgcca cattcgcaac tgccacgccg ccgacccggc
     3541 ctatggcgcc ggcgtggcca aggcgctggg actggatttg caaaaggcgc tggcctcgca
     3601 gaaggacgac ccgatgtttg gcaacccgct ggtggcgctg ccagtctgag catcacgcgg
     3661 cgcgccgctg gcgcgccagc cgcagccgca actgccgcaa cagctccaac cacgccaggc
     3721 agggcagcag cacggcggcg ccggccgcca ggcccggcag acccgggttc cccagcccca
     3781 tcagcgggcc caggccgggg atgaccagca gggccgccag cagcaggccc acggccgcgg
     3841 ccatgggtag cagccagggg ttgggcgcgc cgccgcgccc cggcacccgg ccgtcgcgct
     3901 gcgccaggat gagcagcagc acgcacagca ccagggcgcc aaacaccacc gtgcggccct
     3961 cggccatgct ccagccctgg gcctgcagcc aggcctgcgc gcccagcagc agcgcggcga
     4021 cgcccgcacc ctgcagcagc gccggccata gcgcacgcag ggcaaagggc gagtccgcca
     4081 ccgcgcgcgg cgggcgctgc atgatgtcgg gcgcctcggg ttcggcctcg aagacgatgg
     4141 agcaggccgg gtcgatgagc agctccatca gcacgatgtg caccggcagc agcagcgccg
     4201 gccagtgcag cagcgtgggc gccagcgcca gcgcgatgat gggcacatgc acggcaaaca
     4261 caaagcgcgt ggccttgcgc acgttgtcat cgatgcgccg cccctggcgt atggccgcga
     4321 cgatatgggc aaaactgtcc tgcagcagca ccagcgccgc ggcttcgcgc gccacgtcgg
     4381 tgccacgctc gcccatggca atgccgacat gcgcggcctt gagcgcgggg gcatcgttca
     4441 cgccgtcgcc ggtcatggcc accacggcgc catcgtcgcg cagcagccgc accaggcgca
     4501 gcttgtgcgc gggcttgagg cgtgcgcata tgtccacctg gtgcaggcgt gcgcgcaggg
     4561 cgctgtcgtc caggccctgc agctcatcgc cggtgatgac ctcggggcgc tcggacaggc
     4621 ccacctggcg tgcgatggcg cgcgccgtgg ccgggtggtc gccggtcatc atcaccacgc
     4681 gcacgccggc ggcgcggcat tgcgccaggg cgtcgggcac ctcggggcgc ggcgggtcgg
     4741 ccagcgccag caggcccagg aactcgaagt cgaagtcatg ctggctggcc ggccagagcg
     4801 cgccctgggc cgcgccagtc caatgcccgc gcgccacgcc aagcacgcgc aggccacgtt
     4861 cggccaggga ctcgacctgg gcgcgcaggg cgttgcggcc cgcctcatcg aggtggcaca
     4921 ggtcggccac ggcctcgggc gcgcccttgg tggccagctg gtacatgtgg ggctggccgc
     4981 tggcgaacac gcgtgtcatg gccaggatgt cgctggacag cgggtactcg aactcgggct
     5041 cgcgcccgtc ctgcacatgc tccgtgccct tgagccattc atggccaaag cgcgcaatgg
     5101 ccttctccat ggggtcgaac gggtcggtcg gcgtggccag catggcgaat tcgaccagcc
     5161 gatggaacgg ctcgggcagc gcgttggccc cggccgcctg ccatgtctgt gcgggcgtgg
     5221 ccagctcggc cacggccatg cggttctggg tcagggtgcc ggtcttgtcc accgccagca
     5281 cggtgatggc gcccagggtc tccacggccg tgatgcgtct ggtcagtacc tgctggcgcg
     5341 caatgcgcca ggcacccagg gccaggaaca cggtgaggat gaccggaatc tcttcgggca
     5401 ggatggccat ggccagcgcg atgccggcca gcaggctgtc cagcaggggg cggccattcc
     5461 accaccagcc cagcagcacc tgggccgtgg ccagcagcaa tgccagcgcc cccagggcgc
     5521 gtaccaggcg gcgcgagcgc agctgcagcg gtgaacgcgg cgtctcggtg gccgccaggc
     5581 ccgcaccaat gcggcccacg gccgtgtcgc cggcggtggc cagcacccgc gccaggccca
     5641 cgccgcgcgt gaccacggtg ctggcgtgca ggctgtcggc gcgcgcatca tgagcctctt
     5701 cggccagggc tccatctgac agcggcagcc tggccaccgg cacggattcg cccgtcagca
     5761 gcgactcgtc cacctccagc tggccgttga gcagccgcgc gtctgcggcg atgcggtcgc
     5821 cctcgcgcag caccagcagg tcaccgcgca ccacctcgcg cccggcgatg cgccgctcca
     5881 cgccgtcgcg caacaccagg gcgcgcgggg cggacagctc gcgcagcgac tccagcgcgc
     5941 gttgcgtctt gcgctcctgg gtgagggtga tgcctatgac caccagcacc gagccgagca
     6001 gaaatagcgc ctcgccccgg tcgcccagcg ccagataggt gccgcctgcg gccagcagca
     6061 tcaggaacat gggttcggtc agcacctcgc gcacgatggc caggttggac ttgggcgcgc
     6121 tgcctggcag ctggttcggg ccgtcgctgg ccaggcgctc ggccgcctgt tggctgctca
     6181 ggccctgcag cggctcgtca ggggtgggct ggtctggcat ggggcttggc gcgggtaggg
     6241 gttttacccc agtgtataag ggcggccatt gcctgcagcc acgccccctg accgatgacc
     6301 aagaccgaac accctgccta tttcgaacaa cagcatgagc ggctggacgc gcagctgcat
     6361 gcccatttgc tggatgtcgt gggtggtgat ttcgccggcg cattgcagcg cctgcagcag
     6421 tggcggcgcg acctggaccg gcataccgag atcgagaacg cccgcctgct gccccatgtg
     6481 ccagagggcg cgcgctgggc cgcgcgcgtg tacctgctgg agcatgagcg catcgccctg
     6541 ctggccgacg agtaccagca gcgcgtgcag gccgtcatca atcacccgcc gcaggatgaa
     6601 caggcgcggc gccgcgcggt gctggtgctg ctggacgcgg cccacgccct gcgccatgtg
     6661 ctggagcacc acaacgagcg cgaacatatc gcgctggccc aggagctgcc gctggagctg
     6721 caaaaggcgg cctgggccga cgagccagcg tcccaggtca gaacgccagg gtcttgacgg
     6781 tgggtttaag caggtgagta gccacttccg ctggtttggc ggggctcaca cccggcagca
     6841 gatcgctggg ctgcttgccg gtattgggca ggtagagcgc gcagacctcc acctttgcgc
     6901 cggccttgat aacgccttgc agcatctgct gcggcgtgac gttgcggggc ttgaggattg
     6961 gttgtgcctt ggcggcgtcg gtgttggcca gggcgatatc gccagcggca tcgcacagca
     7021 acacccgcac cggcgctttt tgctccagcg cctggccagc cagcacgagg cctgcgccct
     7081 gtgccatggg gctggcggag tggatggaaa caaacagttc ttcggcatgg atggccgtgg
     7141 ctgcgcaggc gagcaacagg gcagatgcga tcgacttttt catggaaagg ctcctgggtt
     7201 ggggaaggaa gagagcgagc actatatatt agaaacatct aataaatatt gctcgtctga
     7261 aatcagggtc attgccttcc attaatccag ggtgcgggcg tgagccgatg aggcaggcga
     7321 tggcactcga ggcgggcatg ccaggtggtg tgcgtcagtc ctgtgtctgt gcgcccgcga
     7381 cccagcgcag gcccttgccg cggctgaggt agtagtcgcc atcggggctg atcacgacag
     7441 cgcgataagt cgccgggtcg gaaaaatagc gccagccgtg ggctctttcc ccggggccgg
     7501 cgttgtgggc cacgggttgc atgccatggt cgaagccatt gggggccgtt tgctcggtgc
     7561 gctgctggcg ctcatgccgg tcctcacgct cgccggcgag aacgggtgcg ctggccagca
     7621 tggcgatggc ggcgagaaga agggtggtgc gcatgatgat ctccttgacc ggtagtggca
     7681 gtcgggagtg actgtgctgc cagtctgcgc cccgggcgct gaaccggtgc tgaaggcgtt
     7741 gttcaccggc cgttcatgcg ccgccaaagc ggcccaggcc cagttcgacc agcgccccgc
     7801 cgcggcgcag cagccatagc gcctccaggc cgtgccgctg cgcaagcgcc aggccttgcg
     7861 cggcgccggc gacgagcagg gccgtggccc aggcgtcggc cagcatgcag ttggcggcca
     7921 gcacggtcac cgaggccacg tcaccccgca ccggcgtgcc gctgcgcgcg tccatgctgt
     7981 gcgccaggcg ctggccgttc acgtcaaaga agtgccggta gtcacccgac gtggccacgg
     8041 ccagatcctg cagcgcaagc acgccgtgtg cgcggcgcag cccggtctgc ggggattcga
     8101 tggccacggc ccagggtctg ccgtcggcct ggccgccaat ggcgcgcagc tcgccgtcga
     8161 gcgcggccaa cgcctgcgcc acgccgtggt gctgcagcac ctcggccatg cgatccacgg
     8221 cgtagccctt ggcgatgccg cacaggtcga tctgcagcgg cgccagcttg cgtgcgcggc
     8281 ccgcgtgcag atccagctcc agcgtctcgt gggccggccc atgggccagg gcgcgcgcgg
     8341 cgcggatggc gctggcgtcc ggtgcgctgc gtgcggcacc aaagccccag gcgtcaacca
     8401 gtgcacctac gccgatgtcg aaggcgcctt ggctcaatcg cccgatgtcg agcgcgcagg
     8461 ccagtatctg cagcatttcg gtggtcagcg cgaaccaggc gcccacgggc gcctggttca
     8521 gctgcaccag gcagctgtcc gaccgccagg gcgacatctg cgcgtccacc cgttccacgg
     8581 tggcggccag atcgcgttgc agcgctcgcg catcgatctg cggcgccagc tcgcaggttg
     8641 cggaccagcg cgtgcccatg gtggggccgt gcagcaccac gcgggtcgtc tccggtgccg
     8701 cacgctcaga acaggtcttc ggcatagcgt cccgtggcct tgagttcgcg cacgctcagg
     8761 cccagcgtgg cggcgatggt gtccaggacc tcggtcacgg cgctggccat ggggcggctg
     8821 ccgcacacgc gcaccatggc gccaccggcc agcagcgcgc gcaggcgctg cgcgtcgcgg
     8881 cgcaacgcgt cctgcacgta gccgccaccg ccgggcacgc gcgagaaggc ggtgtgcacg
     8941 ctggccacgc gccgttcggt aagccagcgc ttgatgtcgg cgccgaagta gtggtcgtgc
     9001 gccgggtcgc gcgcgcccag gtacaggtgc atgggcgtgc gcctgtcgtt gcgccgtatg
     9061 aagccggcca gcggcgccac ccccgtgccc gcaccgatca gcagcacgct gggctggcgg
     9121 ccctgcaggg caaagcccgg gttggggcgg atgaaggccg gcacctgtgc acccggctgc
     9181 aggccgagca aatgcgtgga gcacaggccg ccgggccatt gacgcacgca gatttccaga
     9241 aagccgtcgg cactgcccga ggccagcgag tagtagcgcg gcacgctgct gcccggcgcc
     9301 aggatggcca ccaggtcgcc cggagcaaac ggcgccaggc catgcccgcg caggcgctca
     9361 aaccagcccg gccggggcca gtcaaagcgc agaatggccg caggacgccc gtcctgcgcg
     9421 ccggggtaat cctggcgcga gcgtagcgtg agtgccgtgg tcggtggcag ctgcggcacg
     9481 tagtccagca ccagcacctg acccagggcc tgggccagcg tcttgcccca gcgggcaaat
     9541 tcctggcccg actgctggtg tatgcattcg agtggcagca gggtcggcca accgcgtgtg
     9601 cgcagctgtg cgtccagagc ctttgcgaag gagcaaaagg ccgggtactg ccggtcgcca
     9661 aagcccagca ccgtcaccgg cagcgggcgc accggctgcg ccgcgatgcg tgcctgcgcc
     9721 tgggcggcat gtgccggcgc ctgcccgtcg ccataggtgg cggccaggat gaacacctgc
     9781 tgtgtcgccg ccgtggtctg gaaattctcc agccccgtgg tgtgtacgcg ccggcccgcc
     9841 tgcagcagtg cggcatgcag cgcctgggca aagccccagg tgctgccgcc ctcgctggcg
     9901 acaaagatca gcgcatcggc ttgcgccagc gggctgttgt gcgcgatgtg caccgcgccg
     9961 cggtgcccct gccaccagat gagcaggccc gtggcccaga acagcagcac gctggcgccc
    10021 gagacgccca gcaccacagc ccagggccag gcgctctcgc ccgtgtgcag cagcatggcc
    10081 cagtcgtaga gccactgcgc gcccgtcgcg tcctgccagg cgagcagcgc gccgctgtgg
    10141 cgatccagcc agccctggcc gctggatgtg gtgacgcgcc aggtatcgct ggtatcggcg
    10201 gcatcgggca ggttgagcct gcgcagctgc gacgtgcgca gctcctgtag caggggaatg
    10261 cggccagctg gcaggtcggc ggcgcctgcc cccgagctca cctcgggctc gggcccggcc
    10321 tccagctgca ccaggcccag cgtgctcgtg ctcatggtca gtgccgtcac cgaggtcaac
    10381 gccaggaatg ccagcagcac ccggccgctc agcacatgca ggcgctgcgc cagtgtgccg
    10441 cgcacccggc ccgtcaactg gcgccagcca cccatgcggc gcagcagcag cacggcgccc
    10501 gagatcgaca gcagcgccag ggccagcgcc gccgccgccg ccccccagcg cccggcgtcg
    10561 cccagcagca gggcgcggtg caggtttttc acccagcgcg gcagcgccga gggctggtag
    10621 gggcccagca gcgcgccgct ggcgggatcg acatacagcg cctgcggctg ctcgccgtca
    10681 aaacccaagg cggcgatacg gccggtgggc aggtggcgga tttcctccac gccgggcata
    10741 tgggcctgca cgcgctcggc cagcgtggcc acgggcagat cgggtgcggc ggccggggcc
    10801 tgccaggcct gatgcacggg gtcgatggcc agcagcgcgc ccgacaggcc gagcaccagt
    10861 gccagcgcgc ccagcgtcag gccgagccag cggtggatgg ttctccagcc catggtggct
    10921 ccttactgca gctgaaagct cagcgactgg atgtactgct tgccggcctg gggcttgccg
    10981 gcgttttgcg tgctcagcgg cacgcgcagc tcggacggac tgtcgcgcat gttttccacg
    11041 gcgacatcga cgtggatctc gtaaccggcg tcgatcagcg cgtcggccag gtcggccgtt
    11101 accttgagcg tgcggcctgc gcccacgctg gcgccggtca cgccgttcag gcgcctggca
    11161 tcgccggccg acaggcggtt ccagcttgcc aggtgcttgt agtacttggc cttgccaccg
    11221 gccacccaca gcgtgccggc gtaggccccc ttggcgtccg tcagatagat ggccaggtag
    11281 gcgccatcgc cgccgtagtt tttcagctga gcgctgatgg tcaccgggcg gctgtgcgcc
    11341 agggtgggca gggccagcgc gcaggccgcg gccagggtgc cgagcagggg tttggacatg
    11401 gtgtgctcct gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcatctg
    11521 gcggaaatag tggtaacaga ccctaatcga tctgaccgcg tggcgaaccg cctggcgtga
    11581 agactggggc cgtggatccc tgggcagggg gcgcgccgtg ctcctgggtg cggcggcgct
    11641 cctggtcacg atggccgtcg cggtgcttga acttcagcac ggccagggtc tcggggtcga
    11701 tcttggcctt gaaacggcgg ccgctggcat cctggccgcg cacctcgtaa cagccatcgt
    11761 cgatcttgat gcgctgcaga tcccagccgc gctgggcggc catctggcgc acggcctcgc
    11821 gcgtctgcca gcgttggctg ggggcgttgc agtcgtcgtc ggccatggcg ggcaggctgg
    11881 ccagtcccag gcacagcgtg agcaggccgt ggcgtacaag ggtgtggggg cgcggcatgg
    11941 tgcttctcct tggtgtatgg gacaggcgct gttcaacgca ttgcaaacga ctcgcggaac
    12001 aggcgccagg gcgcggtgct ggcatgggcg ccaagcgcat cgcccagccc ctgcgggccg
    12061 cagaaccaga tgtccagcgg gccgcccagg gcttccagcg tgcgtggcgt caggcgctgg
    12121 ccccgggcgt cgtcgtgcac cgtgagcgtg accgcgggct cggcctgggc acacagggtt
    12181 tgcaggcgcc ccagcagcgc gtcgcgcgcc gcgtcgcggg tgcagtagtg catctgcacc
    12241 ggttgcagtc tggcgtcggc ggtggccagg cccggctggc gcgcctccag cagggcgatg
    12301 aagggtgtca cgcccacgcc cccagccacc cagacctgct ggcgtgcgcg gctgccgcgc
    12361 ccgtcaaagc ggccgtaggg gccttctatg tccacgcctt ggccggcctg aaggcgccgg
    12421 gccagggtgc gggtgtagtc gcccaggggc ttgatgacca ggcgcagcag ggcctcgccg
    12481 cgtgcgttct cgcccagggc gccgggtgcg ctggcgatgg tgaaggggtg ggcgccctcg
    12541 gcgcggtcaa agcgaacgaa ggcgaactgt ccggggcagt ggcctggcca gctatcgggc
    12601 agggcgcaga ccacctccag tggtgcgtcc ggcccctggc ccaacagctg cacgctgtgg
    12661 atatgcgcgc cgtggcgccg gcgcagccct atcccgcccg ccagcgacca cagcgcggcg
    12721 gcgctgccca gggtcagcag gccggcctgc agcaggccca gcggcagcgc ccagtaggcc
    12781 agcggcgtga gcgccagggc gtggaacaca aagcccaggt agagcagcgg catggcgcgg
    12841 tgcgtcaggc gccagggcct gtaggccagc aggcgctgca gcagggtcag ggcaatcagg
    12901 acgagcaggg cgtagaaggc ccattcgccc agatccttgg ccaggtcgcg cgcgtcggtg
    12961 aaccagggca gcacggcgtc gcgcgccggc ttgccttcgc ggccccaggt ggccttgatg
    13021 aggccgcctc cctccttcac gccccagtgc agtagggcgg tgatgcccgc gcccatgccg
    13081 gcccatttgt gcaggcggta cacctgatcc atgccgccca gcggtcgctc gatcaggggc
    13141 aggcgcaatg ccagcaccat gatcaggccc atcaacccca tgcaccacag gcctgtcaga
    13201 tagatggcgt gccggcgcag ctgccaccag cccgtggcct cgccctgggc attgaccagg
    13261 gggtcaatgc ccagctgggc gggagccacg gcccaccagg ccagggtgat gagcgccagc
    13321 cacaaggcca gggcggtcgc gggggagagg gtacggcgca tgatggactt gcctggttgc
    13381 gtgaaggaga gatggggcgt gcgtcagtcg tgctgcttgc gcgtgcgcgt gcgctccacg
    13441 gcgccggtct gggcgttgac gtagagcttt accggcgcgc cctgggcgtt cagcgccttg
    13501 gcctcgtagc ggccatgctc gtagtcgatc tccaggatgt cgcggtagcc ggcggcctcc
    13561 aggcgctcgg cgatgtcgcg gatggagagc ttttgcgccg gcgcgctggc ggccgcgggg
    13621 ctggccgtgg cggcaggggg gctggtctgt gcctgcgcca ggcccaggcc tatggccgag
    13681 gcggcaatca cggtggcgat gaggcagcgg cgggtggtgg tgctgtgcat ggtgaactcc
    13741 ttgaggttga agaccgccag aaacgacatg cttcgggctt ggattgcatt cttcaaaacc
    13801 atcgctgaac ccaagctgaa ggcgccgttc atctgccatt catcggcgcc gcgctacaac
    13861 cgtgccatgc ctgccctcac ccgccttctc gccctgctcc tgacggcctg cgcgctgtcg
    13921 ctgcccgccg ccgcccatga ccatgatgaa cacgagcgcg cgcgccaggc gctggagcag
    13981 ggccgggtgc tgccgctgac cagcgtcatc gccaaggtgg agcgcgagca cccgggtcag
    14041 gtcgtcaaga tagagttcga ggaggacgac ggccgctaca tctacgaaat ccgcctgctg
    14101 caacgcgacg gccgcatggc caagctcaag gtggatgccg tcgatggccg ggtgttgaag
    14161 atcaaacgca aagaacgcaa ggaataggac gcgccatgcg catactggtg gtcgaggacg
    14221 agcccacgct gcaggcgcag ctggtgcagg ccattgccgg cgccggccac accgtggagg
    14281 ctgcggccga cgggcgcgag gcgcagtacc tgggcgaggt cgaggacttc gacgccgtgg
    14341 tgctggatct gggtctgccg ctgcttgatg gcctgtcggt gctgcgccac tggcgcgcgg
    14401 ccggtcgcag gatgccggtg ctgatcctca ccgcgcgcag cagctggcag gagaaggtgg
    14461 ccggcatgga cgccggcgcc gacgattacc tggccaagcc gtttcatatg gaagagctgc
    14521 tggcgcgcct gcgcgccctg ctgcgccgcc tgggcgagca cgccagcgcc gaatggcagt
    14581 gcggcgccat cagcctggac acgcgcgccg cgcgcgtgct cgtcgccggc cagccgctga
    14641 cactcaccag ccacgagttc aagctgctgg cgcagctgat gcagaggaag ggcgaggtgc
    14701 tctcgcgcac cgaactggcc gagcacctgt acccgcagga cagcgagcgc gactccaaca
    14761 ccatcgaggt cttcgtcggc cggctgcgca agaaactgcc cgagggcagc atagagaccg
    14821 tgcgcggcct gggctaccgg ctgaccgatg gcgacgccgc cgcgccatga aacactcgct
    14881 gcgcctgcgc ctgctggccg gcatgctggt gtggatgctg ctgaccatcg ccgccgccgg
    14941 ctggggcctg cgcgcgctgc tgcaggggca tgtcgagcag cagctggcgg tgcagctggt
    15001 gatggacctg gatcacctga gcggcagcat cgactgggcg ccggacgggc gcctggacgt
    15061 ggaaaaccag cccgacgcgc gcctggcgca gccgctgtcg ggcctgtact ggcagatcga
    15121 ccggctgggc gcccagccgc aggtggcgct ggcgcgctcg cgctcgctgt gggaccagac
    15181 tttgcgcctg cccgccgccc ccagcgccta tccggcgcag ggtcacagcc tgctgcgaat
    15241 ggcggacgca cggggccgcg cactgctggt cgtggcgcgc accgtgcagc tgcccgacga
    15301 gggcgcgccg ccgctgcgcc tggcggtggc tgccgacgag gccctgattg ccgagccgat
    15361 ggcacgcttt acccgcctgt tgctcatcgc cctcggcgcg ctggcgctgg cgctggcgtt
    15421 ggccgtggtg ctgcagctac agctggcgct gcgcccgtta aggcttttgc gagaacgcct
    15481 ggcggccgtg cgcgccgggc aggctgcgcg gctggagggg cgctttgccc aggagcttga
    15541 acccctggtc gccgagttca accatgtgct ggccgagaac gccgacatgg tgcagcgcgc
    15601 ccgcacccag gccggcaacc tggcgcatgc cgtgcacacg ccgctgacca tcctggccaa
    15661 cgccgccgac ggccaggcca cacccctggc gcgcctggtg caagagcagg tcgccagcgc
    15721 ccgccgccag gtcgattacc acctggcgcg cgcccgtgcc gccgccgccg tgcgcgccac
    15781 cggactggcg acgcccgtgc tcgaacccct gcgagcgctc ttgcgcacca tggcgcggct
    15841 gcacggcgcg cgcggcctgt cctttgagct ggccgatggc gctcaagacc tggcctttcg
    15901 tggggagacg caagacctgt acgaaatgct cggcaacctg atggacaacg ccggcaaatg
    15961 ggcgcgcacg cgtgtcgtgg tcgatgtgcg gcgcgagggc cagcagctgt gcttcaccgt
    16021 ggacgacgac ggccccggca ttcccgagga gcagcgccag cgcatgttcg agcgcggcgt
    16081 gcagctcgac gagcgccgca ccggcagcgg cctgggcttg gacatcgtgc gcgccctggc
    16141 cgacagctat ggcggcagcg tgcaggcact tgcatcgccg ttgggcggcg cacgcctggt
    16201 gctgcggctt ccggcggcgg cctgacccgg ttgcgcctga tgctcccaga ggtccagcca
    16261 aaagccaatg ggcaacctca gtctcagact cttttcttgc ctctggggga aggctaagac
    16321 gggaggcaaa ctattatttt tatagctttt tgcgcttgtt gcataagcgt tgcggccact
    16381 tttaactcaa atatcgccgc aggtagcgcc ccgtatggct gccctcctgg gcagccactt
    16441 cttcgggcat gcccacggcc accacgctgc cgccgcccgc gccgccctcg gggcccatgt
    16501 ctatgagcca gtcggccgtc ttgatcacgt ccaggttgtg ctcgatgacg acgatggtgt
    16561 tgccggcgtc gcgcagctgc agcagcaccc tgagcagcag gtctatgtcg gcaaagtgca
    16621 ggccggtggt gggctcgtcc aggatgtaga gggtgcggcc ggtgtcgcgc cgtgacagct
    16681 cctgggccag cttcacgcgc tgggcctcgc cacccgacag ggtggtggcg ctctggccca
    16741 ggcggatgta ggacaggcct acgtcgagca gtgtctgcag cttgcgcgca atcgtcggca
    16801 catccttgaa gaaggcgtag gcgtcctcca ccgtcagctc caggacttgg gcgatgttct
    16861 tgcccttcca ctgcacctcc agcgtctcgc ggttgtagcg ctggccgtgg cagatgtcgc
    16921 agggcacgta gacgtcgggc agaaaatgca tttccacctt gaccacgccg tcaccctggc
    16981 aggcctcgca gcgcccgccc ccggccgatt gcgagacgtt gaaggagaaa cgcccggggc
    17041 cgtagccgcg ctctttggcg gtattcgtct cggccatcag ctcgcggatc ggcgtgaaca
    17101 accccgtgta ggtggccggg ttgctgcgcg gcgtgcggcc tatggggctt tgatcgacgt
    17161 tgatgacctt gtcgaagtat tccagccctt cgatggcatc gtgcggcgcg gcctcctcgt
    17221 gcgcgcggtg gatcaggcgc gcggcggcgc ggtgcagcgt gtcgttgacc agggtgctct
    17281 tgcccgagcc ggacacgccc gtcacgcagg tgagcagccc cacagggaat tcggcgttca
    17341 cgcccttgag gttgttgccc gtggcgccca gcacgcgcac ggcctgcaac tgcccttggg
    17401 tggcgcggtg ctcggccagg cgagccctct tgcgctggct ggcggccgtc tcgggaaagc
    17461 gcgacttcgg cttgttttcg tcctcgggcg cggcgccctg ctgcagcacc ggcagccagg
    17521 gcgtgcgccg cgcgggcacg ggcaaggtgc gcgcgccgct caggtactgg ccggtgagcg
    17581 aagcgggatt cgcgcacagc tcggcataac tgccctgggc catgacacgc ccgccgtgca
    17641 cgcccgcgcc cggccccatg tccacgcagt ggtcggcggc gcggatcatg tcctcgtcgt
    17701 gctccaccac gatgacgctg ttgcccaggt cgcgcaggcg cgtgagggtg gcgatcagcc
    17761 ggtcgttgtc gcgctggtgc aggcctatgc tgggctcgtc cagcacatac atcacgccgg
    17821 tcaggccgga gccaatctgc gaggccaggc ggatgcgctg ggcctcgccg ccggagagcg
    17881 tctcggcgct gcgcgagagg ctgaggtagg acaggcccac gtcgttcaaa aaccccaggc
    17941 gcgcgcctat ctcgcgcacg atcttgtcgg cgatctcggc cttggcgccc gagaggcgca
    18001 gcgcgccgaa ccaggcctgg gcgtcagcca gcgtcatgtc gctcacggcg tagatggcgc
    18061 gcgcggcctc ggcctcgccg accagcacat ggcgcgcctc gcgcctcaat cgcgcgccgc
    18121 cgcattcggc gcagggctgg gtctggcggt agcgcgccag gtcttcgcgc accacgctgg
    18181 agtccgtctc gcgccagcgc cgcgccatgt tcggcaggat gccctcgaag gggtgcttct
    18241 tgacgatggg ctgccccttc tttggcccgc tgtccagcag atagctgaag gcgatgtcct
    18301 cttcgcccga gccgtgcagc accacctggc gcacctgctc gggcagctgc tcgaacggcg
    18361 tgtccacgtc aaagccgtag tgcgccgcca ggctctccag catggcgaag tagtagccgt
    18421 tacgcctgtc ccagcccttg atggcgccac tggccaggct gagtgacgga aaggccacca
    18481 cgcgcgccgg gtcgaacagc tcctgctggc ccaggccgtc gcaggccggg caggcgccgc
    18541 tgggcgagtt gaaggaaaac aggcgcggct ccagctcgct caaggagtag ctgcacaccg
    18601 ggcaggcgaa tttggctgaa aacaaatgct cgcgcccgct gtccatctcc agcgccagca
    18661 cccggcccgc tccttcggcc ccgccgatgc gtagcacggc ctccacgctc tcggccaggc
    18721 gctgctgtgc gtctgcgcgc accttcaggc gatcgaccac cacgtcgatg tcgtgcttct
    18781 cggttttctt caactcgggc aggtcatcga cctcgtaaat ccggccatcg acgcgaaagc
    18841 gcacatagcc ctgtgcctgc atctgctcga acagctcgac gaactcgccc tttttctgcc
    18901 gcgccaccgg ggccagcagc atcaacttgg tgtcctcggg cagctgcagc acggcgtcca
    18961 ccatctggct gacggtctgg gccgccaggg gcaggccgtg atccgggcag tgcggcgtgc
    19021 cagcgcgcgc gtagagcagg cgcaggtagt cgtggatctc ggtcaccgtg cccacggtgg
    19081 agcgcgggtt gtggctggtg gccttctgct cgatgctgat cgccggcgac aggccctcga
    19141 tcagatcgac atcgggcttg t
//
LOCUS       DAOMTQ010000045        19050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_368_length_19050_cov_25.884864, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000045 DAOMTQ010000000
VERSION     DAOMTQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_368_length_19050_cov_25.884864"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK518_06390"
     CDS             complement(<1..284)
                     /locus_tag="PK518_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endoproteinase ArgC"
                     /protein_id="HRM48578.1"
                     /translation="MVAGKTIGWALPVAALLAACGGGGGTDVDGGGGGGPGLAIPTWS
                     QRIEPFEPKAAALKGAAQDSVASRAVAQAGATRVIALAPLQGVTPKRAAAA"
     gene            complement(315..887)
                     /gene="ruvA"
                     /locus_tag="PK518_06395"
     CDS             complement(315..887)
                     /gene="ruvA"
                     /locus_tag="PK518_06395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRM48579.1"
                     /translation="MIGKLTGQLLEKNPPEVLLDCHGVGYEVLVPMSTFYNLPGVGEQ
                     VSLLTQFIVREDAQLLYGFATAQERQAFRELIKISGVGPRTALAVLSGMGVADLAQAV
                     SLQEAGRLVKVPGIGKKTAERLLLELKGKLGVDIGVRQHLVNDAQADILQALQALGYN
                     DKEAAAALKALPADVGVSDGIKLALKSLAR"
     gene            935..1885
                     /locus_tag="PK518_06400"
     CDS             935..1885
                     /locus_tag="PK518_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRM48580.1"
                     /translation="MILRHTFTPHNNTRLSHLCGPADVHLRTIENALQVKIAHRHEQF
                     KVDGPKARATQAMELLQALYEMADHAIKEDHLQLMLAGDASMMDEEGSPAPLTTRRAD
                     LRARTPTQGRYLANIAAHDITFGIGPAGTGKTYLAVACAVDALERSQVQRIVLTRPAV
                     EAGERLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVQKAFERNALEIAPLAFMRGRT
                     LNNAFVILDEAQNTTPEQMKMFLTRIGFGARAVVTGDVSQIDLPKGTLSGLIDAEQVL
                     RRVQGIAITRFTSADVVRHPLVARIVDAYDAQGSRRRTGN"
     gene            1937..2395
                     /gene="ybeY"
                     /locus_tag="PK518_06405"
     CDS             1937..2395
                     /gene="ybeY"
                     /locus_tag="PK518_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRM48581.1"
                     /translation="MALNQLTLSLQFARDATQHRAQLPRHKVARWIRHALAMDAEITV
                     RIVGAEEGQQLNRDYRHKDYATNVLTFDYQQEPTVAADLVLCAPVVEREAREQNKTLE
                     EHYAHLLVHGTLHAQGWDHETSDEDAREMEAYETAIMEELGFADPYLSLG"
     gene            2480..3226
                     /locus_tag="PK518_06410"
     CDS             2480..3226
                     /locus_tag="PK518_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRM48582.1"
                     /translation="MLDTALLLAAAFVAGALNAVAGGGSFLTLPALVFTGVPPVVANA
                     TGTVALLPGYMASAWGFREDMAPPPGLSMRAVVLLSLVGGSAGAALLLVTPDATFKKI
                     IPWLLLAATALFAFGPQLRQWLGAGAHPASAAKAGLGTLAVAAYGGYFNGGLGILLLA
                     LFGLLGQTQLNAMNGMKNLVSALLTAIAVAIYAAGGIVQWQQALIMMIAATLGGYWGA
                     RVARRLPAPALRWGIVATGLVMAGLFFWKQ"
     gene            complement(3294..4541)
                     /locus_tag="PK518_06415"
     CDS             complement(3294..4541)
                     /locus_tag="PK518_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRM48583.1"
                     /translation="MTAFDLQHYQPGEQLYLWWLANPAAPRLIGELRMARSLKGVSLV
                     YAPAWLASGFALSEDLPLMPGEFFPHDKESAAGAVDDARPDRWGERVIRMLDKPPRLA
                     VLDFLFFAGDERFGALGVSVSASDYLARVTGPLPRLADAQEVHKLVAQILAGEPVAPD
                     RRRLIAPGATLGGARPKALLELDGHPWVIKFNEPGEAVDMPLVEHAAMTLAAHAGIHA
                     APTRPVPLQRGHAVAVRRFDRVGGLRRHALSANVALKAAGEAPGYPELAQWLRRRGVT
                     AGGHNLQQMHELFRRMVFNILIDNTDDHEKNHALLMTDSGEYILSPAFDVLPSGQALG
                     YQQMRVGAAGADATLDNALSQCAQFGLKRPEAVAQLRQVCAVVQGWQAHFASVGVTAA
                     DIESLAGQIDRPFLRDQRLAFQR"
     gene            complement(4538..4885)
                     /locus_tag="PK518_06420"
     CDS             complement(4538..4885)
                     /locus_tag="PK518_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009936259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM48584.1"
                     /translation="MPPAVLAQLRALGENLAIARKRRRESVKSWAQRIGVSEPTLARM
                     EKGDPSVSMGVYATALWLMGRSPALPECAAPEHDRGALEDAVRAARARSVRRRPSLYG
                     AAPKPPADEGGTP"
     gene            complement(5053..5505)
                     /gene="dtd"
                     /locus_tag="PK518_06425"
     CDS             complement(5053..5505)
                     /gene="dtd"
                     /locus_tag="PK518_06425"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRM48585.1"
                     /translation="MMGLLQRVRQARVEVAGEVVGQIGPGLLALVCAEQGDGEAEADR
                     LLAKLLKLRIFADAAGKMNLSLQDVAGGLLIVSQFTLAADTTSGNRPGFSQAATPAEG
                     ERLYDYFVAQARAVHPQVATGRFGADMQVHLVNDGPVTIPLRMVPTGT"
     gene            complement(5502..6452)
                     /locus_tag="PK518_06430"
     CDS             complement(5502..6452)
                     /locus_tag="PK518_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRM48586.1"
                     /translation="MPPTVTGLLQVPAWMTPHNLLRASVGVALLTIVLKTLAWWVSGS
                     VGLLSDALESFVNLAGALFALTMVTVAQQPPDAEHPYGHHKAEYFSAGFEGILIVGAS
                     MAILWAAGLRLLHPQPLEQLGWGLGLSLVSTACNGLLAWAMFRSARVYRSVALVGDAR
                     HLITDVWTSVGVVVGLVAAGATGWLWLDPLVAIGVALNILKEGVQLVWRSAQALMDEA
                     VEQELLAQIEAALQRHAQAAGGHVYFDNLTSRRAGARSFLDLHMHVPAHWTLGRAAAA
                     RAAVEAELMDMVPGLYATIELLPEGAETVFEQTEAQEKAT"
     gene            6530..8635
                     /locus_tag="PK518_06435"
     CDS             6530..8635
                     /locus_tag="PK518_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM48587.1"
                     /translation="MSPTSSTSDLTGTGSHACDTLLRLVADTVPVMLAYYDAHQLRCS
                     FANQRFADYVGRALDSLPGTSAQEVLDSETWQQMQPCVQRALAGEAVQHVCQRQQGDG
                     SMRVLEFSLHPDQRMRDGQAAVVGLMVLVNDISRHRQAENALRQSEERMRRFAEVTEE
                     GLVFHRDGTIIDANAAALRMTGYTLAEVLGRSIFDFILPEYRAVSMEYTRRAREHPYE
                     LALYHKSGRAIPIEAVGKTLPQQDGTQRVVIMRDITARREEQEHARFLAQHDTLTQLP
                     NRRHLMRQLAREAVEAEQRQARMAVLFIDLDHFKTVNDSLGHEAGDRLLCEMARRLQE
                     GVNGNDFIARAGGDQFVVLLPDLRYRGSAADMADALIRRLSAAYEIGGTQLSISATVG
                     ISMLPEDGYSPDELLRHAAAAMHYAKEGGRGTHLFYAPYMLGQPAEALQQEHLLRAAV
                     PQDALRLHYQPQIEVSTGRLTGFEALVRWQHPERGLLGPDEFIPLAESRGLVTPIGRW
                     VLREACRQIKAWQDEGLARVPVAVNVSAMEFRQRDLVGEIKQVLADTGLDAQYLEIEI
                     TETTLMQQPEQARTTLDELRALGVGVTVDDFGTGYSSLAYLKRYPLDKIKVDRSFVID
                     TPSDGEDVAIVTAIVQLARSLQLQSVAEGVETPEQLGLLQRLGCELAQGFGIAAPMDA
                     GSAHAWLQMQPPAQTMTEA"
     gene            8661..10157
                     /locus_tag="PK518_06440"
     CDS             8661..10157
                     /locus_tag="PK518_06440"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRM48588.1"
                     /translation="MACIPPPITAPFSEATLHSDPAAALARVQQIYQQQIEHLRAAMQ
                     RFVTGESPTGHVRACYPYVRVQITSVARAATQLAYGFIEGPGDYETTLTRPDLFADYY
                     LEQFRLLQASHGVELEVGLSQQPIPVHFSFAEHDHIEGQLSPERRTLMRDLFDLPDLG
                     AMDDGIANGTWTPRAGEAQPLALFTGPRVDYSLHRLRHYTGTLPEWFQNFVLFTNYQF
                     YIDEFVRLGHAEMAKADSDYIAFIEPGNVVTRRTGLSPEAGDELGTPPPRLPQMPAYH
                     LVRADNSGITMVNIGVGPANAKTITDHIAVLRPHAWMMLGHCAGLRNSQQLGDYVLAH
                     AYVREDHVLDAELPLWVPIPALAEIQVALQQAVADVTQMAGTDLKGIMRTGTVASTDN
                     RNWELLPDNQPQRRFSQSRAVALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIKL
                     PGMANHFYRERVDQHLRIGMRAIDILRAGGPQRLHSRKLRSFAEVAFQ"
     gene            10337..12046
                     /locus_tag="PK518_06445"
     CDS             10337..12046
                     /locus_tag="PK518_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HRM48589.1"
                     /translation="MQQAFAFHPPSTRRMRLLATAGAALALAACGGSDHFDFDRSSKD
                     LAGQKVAAAQIGLPTTGAAVTSVETPTAATGAQPPASIRANIAISPVDTAAPAIKMGL
                     LLPKEWNGKVVMIGGGGYNGSIPSLYTYAAAPAAQAPYPLLSQGYAVFASDSGHQAGA
                     AGSRDGSFGTNDEAVANFSGAALKKVSDVADVLVTRFYGRKPERRYFIGGSTGGREAL
                     AVAQKWPAEWDGVISWYPAWNAASLDLQFGRLSRAFAQPGAYPSLAQRKLVRQAALAQ
                     CDGLDGVSDGLISNVNACNAQFDPATAQHNGKALRCASGANEGDGCLSDAMIAALKVY
                     NSAITFASPLGSGETQYPGFNVWGAELGEDGSSPLQPTVTLLAMNTTPPASPAPLSAP
                     YWAVFWDQWVRYFVTRDAGFDSLSLDPQNPGAWATRINDLTRLQDINSTNLAAFNQRG
                     GKLLMAHGTADVLVSTRATAQYYERLKQTMGEAAVRNFARYYEVPGYGHAASTAFNAN
                     WDSLKALDAWVTQGQAPTAQVVSDTAGVPGRTRPLCEYPSWPRYGGSGDVNKAESFSC
                     VTN"
     gene            12151..13092
                     /locus_tag="PK518_06450"
     CDS             12151..13092
                     /locus_tag="PK518_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM48590.1"
                     /translation="MSVLFEAQNLRKRYGAATVVDGVSFTIAPGECLGVIGPNGAGKT
                     TTIRICLGLTAPDAGSVHFHPGHGAAPLAMPRDALAIKAQLGVVAQFDSLDPDFSCAE
                     NLRVFGRYFGLKGAAMDARIAPLLEFAALAPKADARPGQLSGGMRRRLSLARALVNDP
                     RLLLLDEPTTGLDPQARHLMWERLQTLLQQGKSILLTTHFMDEAERLCSRLLVLDHGR
                     KIAEGRPRELIAEHLEREVVEAYGNGALALAHDARLRPLAARIEVSGETVFFYTQDAR
                     ALLQALAAQPQLRTLHRPANLEDLFLKLTGRQIREDG"
     gene            13094..13906
                     /locus_tag="PK518_06455"
     CDS             13094..13906
                     /locus_tag="PK518_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM48591.1"
                     /translation="MPAPHTNPSLWRAPDLSLRWWPVFLRNLLVWRKLAIPSLMGNIA
                     EPLMWLVAFGYGMGALVGELQVNGLSVPYILFLASGSICMSAMNAASFEALYSAFSRM
                     HVQKTWDGIMNAPISLDSVLLAEMLWAAFKALFTTTAILGVMLALSISHSPKLLAALA
                     VLVFVGIMFASIALIFNALAPGYDFFTYYFTLVLTPMMFLSGVFFPREQLPTAVRIAS
                     DWLPLTNAVELVRPMFMDQWPAQPLRHGLVIAVTTVVAYWLALALTRRRFRA"
     gene            13928..14419
                     /locus_tag="PK518_06460"
     CDS             13928..14419
                     /locus_tag="PK518_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48592.1"
                     /translation="MPALLSPAHIALVDSGISAIVASRDAQLRPSLMRAVGTHISADG
                     QQVSVFLRPSQAGQLLADLAAGGPIAVVFSDPPSNHTLQLKAAGARIRAARPGDQALL
                     RRYLVAMQHCVGQVGYGPAYVAAMLAAPPQDLVAVEFTPESAFDQTPGPRAGTALTPH
                     ATP"
     gene            14416..15759
                     /locus_tag="PK518_06465"
     CDS             14416..15759
                     /locus_tag="PK518_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRM48593.1"
                     /translation="MNDANAPTLRSIRACLEGAIPAVTATCAPDGTPNVAYISQVVYV
                     DEGHVALSFQFFNKTRQNILANPCATVLLLDPVTARYFRLQLRYLRTETAGPLFERMR
                     AQLAGIASHDGMADVFALKGSDVYAVDHIEALPGEGLPAAPARADLLAAVRLCSQRLA
                     RCSGLDELLQALLAGLHEHLAVRHAMVLMLDAQAGLLYTVASFGYAHSGVGSEIRLGQ
                     GVIGMAARETTPVRISHMTHAALYSHALRCSLGESGGTPAHDIPYPGLAAPHSQLALP
                     LISRGRTLGVLFVESPLDLQFGYPDEDALVALAGQLAAAIDLLQAPDEADEAAHTAAV
                     TAAPLGPAVRVRYFAANASVFVNDAYLIKGVAGAILCKLLREHAASGRSEFSNRELRL
                     DPALGLPEVADNLEARLLLLQRRLQESQTGIRLEKAGRGRVRLCLDAPVRLEAAD"
     gene            complement(15769..16209)
                     /locus_tag="PK518_06470"
     CDS             complement(15769..16209)
                     /locus_tag="PK518_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4281 domain-containing protein"
                     /protein_id="HRM48594.1"
                     /translation="MPEALFSFVSLAASVAWLLLALAAPMPMGAARRALLFAAGRGAP
                     VALCAVYMVLLARYWGTTPGGGFRSLGTVLVLLSAPGKMLGAWTHFLAFDLLVGRWMV
                     DDASVPGNARLALVAALPATFLFGPFGLLLYLTGRSLLRSRASP"
     gene            complement(16279..16989)
                     /locus_tag="PK518_06475"
     CDS             complement(16279..16989)
                     /locus_tag="PK518_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48595.1"
                     /translation="MQVNQQEIAALAQAEITPRMDMYAFIHKALRAYMSDTLVGLGRV
                     DTQDDLELAQACARVQQLLEFCRSHLRHENGFVHPAMERHAPGSAAAIAQEHLGHEQA
                     IAMLAAATAELLACPRTRRAPLAHALYQQLALFVAHNFEHMHQEETAHNAVLWAHYSD
                     AELATIEGALVASIPPDEMQVVLRWMLPFMAPAERAAMLADMRRQAPAPVFEGVLQIV
                     RPHLSASEWAKLGRALEQ"
     gene            complement(17090..17809)
                     /locus_tag="PK518_06480"
     CDS             complement(17090..17809)
                     /locus_tag="PK518_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM48596.1"
                     /translation="MITKARIAERIIESILAGKLRPGERLGEQDIADLFSVSRTLVRE
                     ALMQLQARGFVEVRSRVGWYVIEPSFDDALETYAARRVIEPGMLRDAGTPLQTSIRRL
                     RQHVADERKAIAAADAAQRSWLLADFHICLAECLGNRFLTSMLIDLSARTTLVSALYQ
                     SQSEAQTSNDDHAAIVAALADGDNALAERLMLEHIDTLAGRLDETRAGSGSARDRLRS
                     ALAPDMPAAGARATQRRLKKL"
     gene            complement(17862..>19050)
                     /locus_tag="PK518_06485"
     CDS             complement(17862..>19050)
                     /locus_tag="PK518_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HRM48597.1"
                     /translation="ARASFGIWGANLPAIVRAIVAIFWYGAQTAAASSALVALLIRSE
                     GMLQFHQNSHMLGHSTLEVICYVIMWALQLLIIQRGMETMRKFQDWAGPAVWVAMLVL
                     AIGLMVKAGGFSFEHGIPMDVLLEKTKDAGIKGEPGSFWALMAVGATWVTYFAALYLN
                     FCDFSRYAKNREAVTRGNLWGLPVNLIAFSFVAGITTIAAFQVYGEVLLHPEQISAKF
                     DSWFLALLAAMTFAVATLGINVVANFVSSAFDISNVFPRQISFKRGGYIAAAIALLLY
                     PFAPWEGDAAHFVNAVGATMGPLLGIIVVDYYLIARGQINVQALYQELGEYRYQGGWN
                     VNALIATGVGAVFSTILPNFTTLLPAWWGTFGWFIGVAIGGVVYYVLSQLRPRVRAIG
                     AAA"
BASE COUNT         3192 a         6823 c         6210 g         2825 t
ORIGIN      
        1 gcagcggcgg cgcgctttgg cgtcacgccc tgcagcggcg ccagcgcaat cacccgcgtg
       61 gcgccggcct gggccacggc gcggcttgcc acactgtcct gggccgcgcc tttgagagcc
      121 gcggccttgg gctcgaaagg ctctatgcgt tggctccagg tcgggatggc aaggccgggg
      181 ccaccgccac caccaccatc gacatcggta ccgccgccgc cgccgcacgc ggccagaagg
      241 gcggccaccg gcagcgccca ccctattgtt tttccggcaa ccatcgtcac acctccatgg
      301 ccctgccaca tgatttatct ggccagcgac ttcagcgcca gcttgatgcc atcgctcacg
      361 cctacgtcgg ccggcagcgc cttcagggcg gcggcggcct ccttgtcgtt atagcccagc
      421 gcctgcagcg cctgcaggat gtcggcctgc gcatcgttaa ccagatgttg ccgcacgccg
      481 atgtccacac ccagcttgcc cttgagctcc agcagcaggc gctcggccgt cttcttgcct
      541 atgcccggca ccttgaccag gcgcccggcc tcctgcagcg acacggcctg ggccagatca
      601 gccacgccca tgcccgagag caccgccagc gccgtgcgcg gccccacgcc gctgatcttg
      661 atgagttcgc gaaacgcctg gcgctcttgc gccgtggcaa agccgtagag cagctgcgcg
      721 tcctcgcgca cgatgaactg ggtcagcagg ctgacctgct cgcccacgcc gggcaggttg
      781 tagaaggtgc tcatgggcac cagcacctcg tagcccacgc cgtggcagtc cagcagcacc
      841 tcggggggat tcttctccag cagctggccc gtcaatttgc ctatcatccc aatcctttac
      901 cgcagtcaac gcggccatcc agggaattat cagcgtgatc ctgcgccaca ccttcacccc
      961 ccacaacaac acccgcctgt cgcacctgtg cggcccggcc gatgtacacc tgcgcaccat
     1021 agagaacgcg ctgcaggtca agatcgccca ccgccacgag cagttcaagg tggacggccc
     1081 caaggcccgc gccacccagg ccatggaact gctgcaggcg ctgtacgaaa tggccgacca
     1141 cgccatcaag gaggatcacc tgcagctgat gctggccggc gacgccagca tgatggacga
     1201 agagggcagc ccggccccgc tgaccacgcg ccgcgccgac ctgcgcgcgc gcacgcccac
     1261 ccagggccgc tacctggcca acatcgccgc gcacgacatc accttcggca taggcccggc
     1321 cggcaccggc aagacctacc tggccgtggc ctgcgccgtc gatgcgctgg aacgcagcca
     1381 ggtgcagcgc atcgtgctca cccgcccggc ggtggaggcc ggcgagcgcc tgggctttct
     1441 gcccggcgac ctgacgcaaa aggtagaccc ctacctgcgc ccgctgtacg acgcgctgta
     1501 cgacctgatg ggttttgacc gcgtgcagaa ggccttcgaa cgcaacgcac tggagatcgc
     1561 gccgctggcc ttcatgcgcg gccgaacgct gaacaacgcc ttcgtcatcc tggacgaggc
     1621 ccagaacacc acgcccgagc agatgaagat gttcctcacg cgcatcggct ttggcgcacg
     1681 cgccgtggtc acgggcgacg tgagccagat cgatctgccc aagggcacat tgagcggcct
     1741 gatcgatgcc gaacaggtac tgcggcgcgt gcagggcatc gccatcacgc gcttcaccag
     1801 cgccgacgtg gtgcgccacc cgctggtggc acgcatcgtg gacgcctacg acgcccaggg
     1861 cagccgccgc cgcaccggca actaactcct gttttaatag ctatttgcgc ttgatacaca
     1921 ggcgttggag gccaatttgg cactgaatca actgaccctc tccctgcagt tcgcgcgcga
     1981 tgccacgcag caccgcgccc agctgccgcg ccacaaggtg gcacgctgga tacgccacgc
     2041 cctggccatg gacgccgaaa tcaccgtgcg catcgtcgga gccgaggaag gccagcagct
     2101 caaccgcgac taccgccaca aggactacgc caccaacgtg ctgaccttcg actaccagca
     2161 ggagccgacc gtggccgccg acctggtgct ctgcgccccg gtggtcgagc gcgaggcgcg
     2221 cgagcagaac aagacgctgg aagaacacta cgcccacctg ctggtgcacg gcaccctgca
     2281 cgcccagggc tgggatcatg aaaccagcga cgaggacgcg cgggagatgg aggcgtatga
     2341 gacggccatc atggaagagt tggggtttgc ggatccatat ctgtcgcttg ggtaagtggt
     2401 aaatggtgag tggcaagtcg tgaataacga cagcgccgcc accaaccact tactactgac
     2461 cattgagcac ttgcccccca tgctcgacac cgccctcctg cttgccgctg ccttcgtcgc
     2521 cggcgccctc aacgccgtcg ccggcggcgg cagctttttg acgctgccag ccttggtctt
     2581 caccggtgtg ccgccggtgg tggccaacgc caccggcacc gtggcactgc tgcccggcta
     2641 catggccagc gcctggggct ttcgcgagga catggcgccg ccgccggggc tgtcgatgcg
     2701 cgccgtggtg ctgctgtcgc tcgtcggcgg ctcggccggg gcggcgctgc tgctggtcac
     2761 gcccgacgcc accttcaaga agatcatccc ctggctgctg ctcgccgcca ccgcgctgtt
     2821 tgcgtttggc ccgcagctgc gccagtggct cggcgccggc gcgcacccgg ccagcgccgc
     2881 caaggccggc ctgggcacgc tggccgtggc ggcctacggc ggctacttca acggtggcct
     2941 gggcatcttg ctgctggcac tgttcggcct gctgggccag acccagctca acgccatgaa
     3001 cggcatgaag aacctggtct cggccctgct caccgccatc gccgtggcca tctacgccgc
     3061 tggcggcatc gtgcagtggc agcaggcgct catcatgatg attgccgcca ccctgggcgg
     3121 ctactggggc gcccgcgtgg cgcgccgcct gccggcgccg gcgctgcgct ggggcatagt
     3181 cgccaccggg ctggtgatgg cggggctgtt tttctggaag cagtagcctc cccaaataaa
     3241 aaccggccat cagcgccaat acacaagcgc tgaaagctat tgtttacaga gcatcagcgc
     3301 tgaaatgcca gccgctggtc gcgcaaaaac ggccggtcga tctggcccgc cagcgattcg
     3361 atgtcggccg ccgtcacgcc cacgctggca aagtgcgcct gccagccctg cacgaccgcg
     3421 cacacctgcc gcagctgggc cacggcctcg ggccgcttca ggccaaactg cgcgcattgc
     3481 gacagggcgt tgtccagcgt ggcatccgcg cccgccgcgc ccacgcgcat ctgctgatag
     3541 cccagcgcct gacccgaggg caacacgtcg aacgcgggcg acaggatgta ctcgccgctg
     3601 tcggtcatca gcagcgcgtg gttcttctcg tggtcgtcgg tgttgtcgat gaggatgttg
     3661 aagaccatgc ggcgaaacag ctcgtgcatc tgctgcaggt tgtgcccgcc tgccgtaacg
     3721 ccgcgccggc gcagccactg ggccagctcg gggtagcccg gcgcctcgcc cgcggccttg
     3781 agcgccacgt tggccgaaag tgcgtggcgg cgcagccccc ccacgcggtc gaagcggcgc
     3841 acggccacgg cgtggccccg ctgcagcggc acgggtcgcg tgggcgcggc gtgtatgccc
     3901 gcgtgcgccg ccagcgtcat ggcggcgtgc tcgaccagcg gcatgtcgac ggcctcgccc
     3961 ggctcgttga acttgatcac ccaggggtgg ccgtccagct ccagcagcgc cttggggcgt
     4021 gcgccgccca gggtggcgcc cggcgcgatc aggcgccgcc tgtctggcgc cacgggttcg
     4081 ccagccagaa tctgcgccac cagcttgtgc acttcctgcg catcggccag gcgcggcagc
     4141 gggccggtga cgcgcgccag gtagtcgctg gccgagacgg acacgcccag ggcgccaaag
     4201 cgctcgtcgc cggcaaagaa caagaagtcg agcacggcca ggcgcggcgg cttgtccagc
     4261 atgcggatga cgcgctcgcc ccagcggtcg ggccgcgcgt cgtcgacggc gccggcggcg
     4321 ctttccttat cgtgcgggaa gaactctccc ggcatcaacg gcaggtcttc gctgagcgca
     4381 aagccgctgg ccagccacgc tggcgcatag accagcgaca cgcccttgag gctgcgcgcc
     4441 atgcgcagct caccgatcag gcgcggcgcc gccgggttgg ccagccacca caggtagagc
     4501 tgctcgccag gctggtagtg ctgcaggtca aaggccgtca tggcgttccg ccttcatccg
     4561 ccgggggttt gggcgccgcg ccgtagagcg acggccgccg gcgcaccgag cgcgcccggg
     4621 cggcacgcac ggcgtcttcc agcgcgccgc ggtcatgctc gggcgcggcg cattcgggca
     4681 gcgccggcga gcgccccatc agccacaggg ccgtcgcata cacgcccatg gagacgctgg
     4741 ggtcgccctt ttccatgcgc gccagcgtgg gctccgagac accgatgcgc tgcgcccacg
     4801 acttcacgct ctcgcgccgg cgcttgcgcg caatggccag gttctcgccc agcgcgcgca
     4861 gctgcgccag cacggcgggc ggcatggcaa ccaggctaga agatcgcttt gacattgcat
     4921 atatgttagt gcaaagcgaa aaaaccacat atatatgttt gtttaagaca attgccacag
     4981 cagcaggccg cgcagcaaaa gtgcctcaag cacttatgga taaagcgcat caagctatat
     5041 tttgaatagc aatcaagtgc ctgtcggcac catgcgcagc ggtatcgtca ccggcccgtc
     5101 attgaccagg tgcacctgca tgtcggcgcc aaagcggccc gtggccacct gcgggtgaac
     5161 ggcgcgcgcc tgggcgacga agtagtcgta caggcgctcg ccctcggccg gggtggcggc
     5221 ctggctgaag cccgggcggt tgccgctggt ggtatcggcg gccagggtga attggctgac
     5281 gatgagcagg ccgccagcca cgtcctgcag gctcagattc atcttgccgg cggcgtcggc
     5341 aaagatgcgc agcttgagca gcttggccag caggcggtcg gcctcggcct cgccgtcgcc
     5401 ctgctcggcg cataccaggg ccagcaggcc ggggccgatc tggcccacca cctcgcccgc
     5461 cacctccacg cgcgcctggc gcacgcgctg caacagtccc atcacgtcgc cttctcctgt
     5521 gcctcggttt gctcaaaaac ggtttccgca ccctcgggca gcagctcaat ggtggcgtac
     5581 aggccgggca ccatgtccat cagctcggcc tcgacggcgg cgcgcgcggc ggcggcgcgg
     5641 cccagcgtcc aatgcgccgg cacatgcatg tgcaggtcga gaaaactgcg cgcgccggcc
     5701 cggcgcgagg tcaggttgtc gaagtacaca tggccacccg cggcctgcgc gtggcgctgc
     5761 agcgcggcct cgatctgtgc cagcagctcc tgctccacgg cctcgtccat cagagcctgg
     5821 gccgagcgcc agaccagctg cacgccctcc ttcagaatat tcagcgccac gccaatggcc
     5881 accagcggat cgagccacag ccagccggtg gcaccggcgg ccaccaggcc cacgaccacg
     5941 cccaccgagg tccagacatc ggtgatgaga tggcgcgcat cccccaccag cgccaccgag
     6001 cggtagacgc gcgccgagcg gaacatggcc caggccagca ggccgttgca ggccgtgctg
     6061 accagggaca ggcccaggcc ccagcccagc tgctccagcg gctgcgggtg cagcaggcgc
     6121 agccccgccg cccacaggat ggccatgctg gcgccgacga tgaggatgcc ctcgaagcct
     6181 gccgagaaat actccgcctt gtggtggcca taggggtgct cggcgtccgg cggctgctgc
     6241 gccaccgtga ccatggtgag ggcgaacagc gcgccggcca ggttcacaaa ggattcgagc
     6301 gcatccgaca acagccccac cgagccgctg acccaccagg ccagggtctt gaggacgatg
     6361 gtcagcagcg ccacgcccac cgaggcgcgc agcaggttgt gcggcgtcat ccaggcaggg
     6421 acttgcaaca atccggtaac agtaggtggc atggcgcaaa gtatgcactt gccacatcgt
     6481 caaagcgccg gcaatccgct ataaatggcg gttacaccgc ccaccaccca tgtcaccgac
     6541 ctcctccacc tcggacttga ccggcacggg tagccacgcc tgcgatacgc tgctgcgcct
     6601 ggtggccgac acggtgccgg tgatgctggc gtattacgac gcccaccaac tgcgctgcag
     6661 ctttgccaac cagcgttttg ccgactatgt cggccgggcg ctggacagcc tgcccggcac
     6721 ctcggcacaa gaggtgctgg acagcgaaac ctggcaacaa atgcaaccct gtgtgcagcg
     6781 cgcactcgcc ggcgaggccg tgcagcatgt ctgccaacgc cagcagggcg acggcagcat
     6841 gcgcgtgctg gagttcagcc tgcaccccga ccagcgcatg cgcgatggcc aggccgccgt
     6901 ggtcggcctg atggtgctgg tgaacgacat cagccgccac cgccaggccg aaaacgccct
     6961 gcgccagagc gaggaacgca tgcgccggtt tgccgaggtc accgaggagg gtctggtgtt
     7021 ccatcgggac ggcaccatca tcgacgccaa cgccgccgcc ctgcgcatga ccggctacac
     7081 gctggccgaa gtcctgggcc gctcgatttt cgacttcatc ctgcccgagt accgcgcggt
     7141 gtccatggag tacacgcgcc gcgcgcgcga acacccgtac gagctggcgc tgtaccacaa
     7201 gagcggtcgc gccattccca tagaggccgt gggcaagacc ctgccgcagc aggatggcac
     7261 gcagcgcgtg gtcatcatgc gcgacatcac ggcgcggcgc gaggagcagg agcatgcccg
     7321 ctttctggcc cagcacgaca ccctgaccca gctgcccaac cgccgccacc tgatgcgcca
     7381 gctggcgcgc gaggccgtgg aggccgagca gcgccaggcg cgcatggccg tgctgttcat
     7441 cgacctggat catttcaaga ccgtcaacga ctcgctgggc cacgaggccg gcgaccggct
     7501 gctgtgcgag atggcgcggc gcctgcagga gggcgtgaac ggcaacgact tcatcgcccg
     7561 cgccggcggc gaccagttcg tggtgctgct gccagacctg cgctaccgcg gctcggcggc
     7621 cgacatggcg gacgcgctga tccggcgcct gagcgccgcg tatgagattg gcggcacgca
     7681 gctgtcgatc tcggccacgg taggcatcag catgctgccc gaggacggct acagccccga
     7741 cgagctactg cgccacgccg ccgccgccat gcactacgcc aaggagggcg ggcgcggcac
     7801 gcacctgttc tacgcgccct acatgctggg gcagccggcc gaggcgctgc agcaggagca
     7861 cctgctgcgc gcggccgtgc cgcaggacgc gctgcgcctg cactaccagc cgcagatcga
     7921 ggtcagcacc gggcggctga cgggcttcga ggccctggtg cgctggcagc accccgaacg
     7981 cggcctgctg gggccggacg agttcatccc cctggccgag tcgcgcggcc tggtcacccc
     8041 cataggccgc tgggtgctgc gcgaggcctg ccgccagatc aaggcctggc aggacgaggg
     8101 actggcgcgc gtgccggtgg cggtgaacgt gtcggccatg gagtttcgcc agcgcgacct
     8161 cgtcggtgaa atcaagcagg tgctggccga caccggcctg gacgcgcagt acctggagat
     8221 cgagatcacc gagaccacgc tgatgcagca gcccgagcag gcgcgcacca cgctggacga
     8281 gctgcgggcc ctgggcgtgg gcgtgacggt ggacgacttc ggcaccggct actcgtcgct
     8341 ggcctacctc aaacgctacc cgctggacaa gatcaaggtg gatcgatcct tcgtcatcga
     8401 cacccccagc gacggcgagg acgtggccat cgtcaccgcc atcgtgcagc tggcgcgcag
     8461 cctgcagctg cagtcggtgg ccgagggcgt ggagacgccc gagcagctgg gcctgctgca
     8521 gcgcctgggt tgcgagctgg cccagggctt tggcattgcc gcacccatgg atgcgggcag
     8581 cgcgcatgcc tggctgcaga tgcagccgcc cgcgcaaacc atgacagaag cgtgacagcg
     8641 ggccatggac aatcatgccc atggcctgca tccccccacc catcaccgcc cccttctccg
     8701 aagccacgct gcacagcgac cccgccgccg ccctggcccg ggtgcaacag atctaccagc
     8761 agcagatcga gcacctgcgc gccgccatgc agcgctttgt caccggtgaa tcgcccaccg
     8821 gccatgtgcg cgcctgctac ccctatgtgc gcgtgcagat caccagcgtg gcgcgcgccg
     8881 ccacgcagct ggcctacggc ttcatcgagg ggccgggcga ctacgagacc acgctcaccc
     8941 gcccggatct gtttgccgac tattacctgg agcagttccg cctgctgcag gccagccacg
     9001 gggtggagct ggaggtgggt ttgagccagc agccgatccc ggtgcatttc tcgtttgccg
     9061 agcatgacca tatcgagggc cagctcagcc ccgagcggcg cacgctgatg cgcgacctgt
     9121 tcgacctgcc ggatctgggc gccatggacg acggcatcgc caacggcacc tggacgccgc
     9181 gcgcgggcga ggcccagccg ctggcgctgt tcaccggccc gcgcgtggat tactcgctgc
     9241 accgcctgcg ccactacacc ggcacgcttc ccgaatggtt ccagaacttc gtgctgttca
     9301 ccaactacca gttctacatc gacgagttcg tgcgcctggg ccatgccgag atggctaagg
     9361 cagacagcga ctacatcgcc ttcatcgagc ccggcaacgt ggtcacgcgc cgcaccggcc
     9421 tgtcgcccga ggccggcgac gaactcggca cgccgccgcc gcgcctgccg cagatgcccg
     9481 cctaccacct ggtgcgcgcc gacaacagcg gcatcaccat ggtcaacatc ggcgtcggcc
     9541 cggccaacgc caagaccatc accgaccata tcgccgtgct gcgcccgcat gcctggatga
     9601 tgctgggcca ctgcgccgga ttgcgtaaca gccagcagct cggtgactac gtgctggccc
     9661 atgcctatgt gcgcgaagac catgtgctgg acgccgaact gccgctgtgg gtgcccatcc
     9721 cggctctggc cgagatccag gtcgcgctgc agcaggccgt ggccgatgtg acgcagatgg
     9781 ccggcaccga cctcaagggc atcatgcgca ccggcaccgt ggccagcacc gacaaccgca
     9841 actgggagct gctgcccgac aaccagcccc agcgccgctt cagccaaagc cgcgccgtgg
     9901 cgctggacat ggaaagcgcc accatcgccg ccaacggctt ccgcttccgc gtgccctacg
     9961 gcaccctgct gtgcgtcagc gacaagccgc tgcacggcga gatcaagctg cccggcatgg
    10021 ccaaccattt ctaccgcgag cgcgtggatc agcacctgcg cattggcatg cgcgccatcg
    10081 acatcctgcg cgccggcggc ccgcagcgcc tgcacagccg caagctgcgt agctttgccg
    10141 aggtggcgtt tcagtaattt tgatagctat ccgcgcacaa ctgacaagcg tttgaagcca
    10201 aaccataccc catcaatttt ggggtatgga gaactacgta acggcaaacc ccgcttgccc
    10261 agggtgctgc atcactccaa gaatggccgc cggccggcat gcgcccggcc cgccacattt
    10321 caaaggagac aagcagatgc agcaagcctt cgccttccac cccccatcga cacgccgcat
    10381 gcggctgctg gccacggccg gcgcggcact ggctctggcc gcctgtggcg gctcggacca
    10441 cttcgacttc gaccgctcca gcaaggatct ggcggggcag aaggtcgccg ccgcccagat
    10501 cggcctgccg accacgggcg cggccgtcac ctcggtggag acacccaccg ccgccaccgg
    10561 agcgcagccc cccgcgtcga tacgcgcaaa catcgccatt tcgcccgtgg atacagcggc
    10621 gccagctatc aaaatgggac tgcttctgcc caaggaatgg aatggcaagg tggtcatgat
    10681 tggcggcggc ggctacaacg gcagcatccc cagcctctac acctacgccg cggcaccagc
    10741 ggcccaggcc ccctacccct tgttaagcca gggctacgcc gtgttcgcca gcgactccgg
    10801 ccaccaggcg ggcgccgccg gcagccgcga cggcagcttt ggcacgaatg acgaggccgt
    10861 ggccaacttc tccggcgcgg cgctgaagaa agtcagcgac gtggccgacg tgctcgtcac
    10921 gcgcttttat ggccgcaagc ccgagcggcg ctacttcatc ggcggctcca ccggcgggcg
    10981 cgaggccctg gccgtggcgc agaaatggcc ggccgaatgg gacggcgtga tctcctggta
    11041 cccggcctgg aacgccgcca gcctggacct gcaattcggc cgcctgagcc gcgccttcgc
    11101 ccagcccggc gcctacccca gccttgccca gcgcaagctt gtgcgccagg cggccctggc
    11161 ccaatgcgat ggtctggacg gcgtctccga tggcctcatc agcaacgtca acgcctgcaa
    11221 cgcccagttc gacccggcga cggcgcaaca caacggcaag gcgctgcgct gcgccagcgg
    11281 cgccaacgag ggcgatggct gcctgtccga cgccatgatc gccgcgctca aggtctacaa
    11341 cagcgccatc accttcgcca gcccgctggg cagcggcgag acgcagtacc cgggcttcaa
    11401 cgtctggggc gccgaactgg gcgaggacgg cagcagcccg ctgcagccca ccgtgaccct
    11461 gctggccatg aacacgacgc ccccggccag cccggcgccc ttgagcgcac cctactgggc
    11521 cgtattctgg gatcaatggg tgcgctactt cgtgacgcgc gacgcgggct ttgattcgct
    11581 ctcgctcgac ccgcaaaacc ccggcgcctg ggccacgcgc atcaacgatc tgacccggct
    11641 gcaggacatc aacagcacca acctggcggc cttcaaccag cgcggcggca agctcttgat
    11701 ggcccatggc accgccgatg tgctggtgag cacgcgcgcc accgcccagt actacgagcg
    11761 cctgaagcaa accatgggcg aagcggcggt gaggaacttt gcccgctact acgaggtgcc
    11821 gggctacggc catgccgcca gcacggcctt caacgccaac tgggactcac tcaaggccct
    11881 ggacgcctgg gtcacccagg gccaggcgcc caccgcccag gtggtgagcg acaccgccgg
    11941 cgtgcccggc cgcacccggc cgctgtgcga ataccccagc tggccgcgct atggcggcag
    12001 cggcgacgtg aacaaggccg agagcttcag ctgcgtgacc aactgacaga aagctattaa
    12061 aacaatagct gtttgcgctt gatggatggg cgcaaacagc tatttttgct tcaaacacca
    12121 ggcgcgccgc tttcccccac aatcacgggc atgagcgtgc tcttcgaagc ccagaacctg
    12181 cgcaagcgct acggcgccgc caccgtggtg gacggcgtgt cgttcaccat cgcgcccggc
    12241 gaatgcctgg gcgtgatcgg ccccaacggc gcgggcaaga ccaccaccat acgcatctgc
    12301 ctggggctca ccgcacccga cgccggcagc gtgcacttcc accccggcca tggcgccgcg
    12361 ccgctggcca tgccgcgtga cgcgctggcc atcaaggcgc agctgggcgt ggtggcacag
    12421 ttcgacagcc tggaccccga tttcagctgc gccgagaacc tgcgcgtgtt cgggcgctac
    12481 tttggcctca agggtgcagc catggacgcg cgcatcgcgc cgctgctgga gtttgccgcg
    12541 ctcgcgccca aggccgacgc gcgcccgggc cagctctcgg gcggcatgcg ccggcgcctg
    12601 tcgctggcgc gcgccctggt caacgaccca cgcctgttgc tgctggacga gcccaccacg
    12661 ggcctggacc cgcaggcgcg ccacctgatg tgggagcggc tgcaaacgct gctgcagcag
    12721 ggcaagtcca tcctgctgac cacgcatttc atggacgagg ccgagcgcct gtgctcgcgc
    12781 ctgctggtgc tcgaccatgg ccgcaagatc gccgagggcc gcccgcgcga gctgatcgcc
    12841 gagcacctgg aacgcgaggt ggtcgaggcc tacggcaacg gcgcgctggc cctggcacat
    12901 gacgcccggc tgcgcccgct ggcggcgcgc atcgaggtca gcggcgagac ggtgttcttc
    12961 tacacccagg atgcgcgcgc cctgctgcag gcgctggccg cccagccgca gctgcgcacc
    13021 ctgcaccggc cggcgaacct ggaagatctg ttcctcaagc tcaccggccg gcagatccgc
    13081 gaggatggtt gatatgcccg caccccacac gaatccaagc ctctggcgcg cccccgacct
    13141 gtcgctgcgc tggtggccgg tgtttttgcg caacctgctg gtctggcgca agctggccat
    13201 acccagcctg atgggcaaca tcgccgagcc gctgatgtgg ctggtggcct ttggctacgg
    13261 catgggcgcg ctggtgggcg agctgcaggt gaacgggctg agcgtgccct acatcctgtt
    13321 cctggccagc ggctcgatct gcatgagcgc catgaacgcc gccagcttcg aggccctgta
    13381 ctcagccttc tcgcgcatgc atgtgcaaaa gacctgggac ggcatcatga acgcccccat
    13441 cagcctggac agcgtgctgc tggccgagat gctgtgggcc gccttcaagg cgctgttcac
    13501 gaccacggcc atcctggggg taatgctggc gctgtccatc agccacagcc cgaagctgct
    13561 ggcggcgctg gccgtgctgg tgttcgtggg catcatgttt gccagcatcg cgctcatctt
    13621 caacgcgctg gcgccgggct atgacttctt cacctactac ttcacgctgg tgctcacgcc
    13681 catgatgttt ctgtcgggtg tgttcttccc gcgcgagcag ctgccgacgg cggtgcgcat
    13741 cgcctcggac tggctgccgc tgaccaatgc cgtggagctg gtgcgcccca tgttcatgga
    13801 ccagtggccg gcgcagccgc tgcgccacgg cctggtgatc gccgtgacca ccgtggtcgc
    13861 ctactggctg gcgctagccc tgacgcgccg gcgctttcgc gcctgaccgc agacaatggc
    13921 cagcaccatg cccgcgctgc tcagccccgc ccatatcgcc ctggtggaca gtggcatctc
    13981 ggccatcgtc gcctcgcgcg acgcccagct gcgccccagc ctgatgcgcg ccgtgggcac
    14041 gcacatcagc gcggacgggc agcaggtcag cgtcttcctg cgccccagcc aggccgggca
    14101 gctgctggcc gacctggcgg cgggcggccc gatcgccgtg gtcttcagcg acccgccatc
    14161 gaaccacaca ctgcagctca aggccgccgg cgcacgcata cgcgcggccc ggcccggcga
    14221 ccaggcgctg ctgcggcgct acctggtggc catgcagcat tgcgtgggcc aggtgggcta
    14281 cggccccgcc tacgttgccg ccatgctggc cgcgccgccg caggatctgg tggccgtgga
    14341 gttcacgccc gagtcggcct tcgaccagac accaggcccg cgtgcaggca cagcactgac
    14401 cccgcacgcc acaccatgaa cgacgccaac gcccccacgc tgcgctccat ccgtgcctgc
    14461 ctggagggcg cgataccggc cgtcaccgcc acctgcgccc cggacggcac gcccaacgtg
    14521 gcctacatct cgcaggtggt ctacgtcgat gagggccatg tggcgctgtc gttccagttc
    14581 ttcaacaaga cgcgccagaa catcctggcc aacccctgcg ccacggtgct gctgctcgat
    14641 ccggtcacgg cacgctactt tcgcctgcaa ctgcgctacc tgcgcaccga gaccgccggg
    14701 ccgctgttcg agcgcatgcg cgcgcagctg gccggcattg cctcgcacga cggcatggcg
    14761 gatgtgttcg cgctcaaagg ctcagacgtc tacgccgtcg accacatcga ggccctgccc
    14821 ggcgagggtc tgccagcggc gcccgcacgc gccgacctgc tggccgcggt acgcctgtgc
    14881 agccagcgcc tggcgcgctg cagcggcctg gacgaactgc tgcaggcgtt gctggccggc
    14941 ctgcacgaac acctcgccgt gcgccacgcc atggtgctga tgctcgatgc acaggcgggc
    15001 ctgctctata ccgtggccag ctttggttac gcgcattcgg gcgtgggttc ggagatccgt
    15061 ctgggccagg gcgtgatcgg catggccgcg cgcgagacca cgccggtgcg catcagccac
    15121 atgacgcacg ccgcgctcta cagccatgca ttgcgctgca gcctgggcga gagcggcggc
    15181 accccggcgc acgacatccc ctaccccggc ctggcggcgc cgcacagcca gctggccctg
    15241 ccactgatca gccgcgggcg cacgctgggc gtgctgttcg tggaaagccc gctggatctg
    15301 cagtttggct accccgacga ggacgcgctg gtggcgctgg ccggccagct ggcggccgcc
    15361 atcgatctgc tgcaggcacc cgacgaagcg gacgaggccg cgcacaccgc cgcggtcacg
    15421 gccgcgcccc tgggccccgc tgtgcgggtg cgctacttcg ccgccaacgc cagcgtcttt
    15481 gtcaacgacg cctacctgat caagggcgtg gccggcgcca ttctctgcaa gctgctgcgc
    15541 gaacacgctg ccagcggtcg cagcgagttc agcaaccgcg agctgcgcct ggacccggcc
    15601 ctgggtctgc ccgaggtggc cgacaacctg gaggccaggc tgctgctgct gcagcggagg
    15661 ttgcaggaaa gccagaccgg gatccggctg gaaaaagccg gccgtggccg cgtgcgcctg
    15721 tgcctggatg caccggtgcg actggaggcg gccgactagc gcacacgctc agggcgacgc
    15781 ccggctgcgc agcaagctcc gtccggtcag atacagcagc aacccgaacg ggccgaacaa
    15841 aaacgtcgcc ggcagcgccg caaccagggc caggcgcgcg ttgcctggca ccgaggcgtc
    15901 atcgaccatc cagcggccga ccagcagatc gaaggccagg aaatgcgtcc aggcgccaag
    15961 catcttgccg ggcgcggaga gcagcaccag cacggtgccc agcgaccgaa atcccccgcc
    16021 cggcgtcgtg ccccagtagc gcgccagcag gaccatgtaa acggcacaca gggcgacagg
    16081 cgcgccgcgg cccgcagcga acaacagcgc gcggcgcgcc gcccccatgg gcatgggtgc
    16141 ggccagggcc agcagcagcc aggccacgct ggccgccagg ctgacgaatg aaaacagggc
    16201 ttctggcatg gctgtggcga tgcggttgtc ggtacgatgc cgcgcctcct gcgcgtgcgg
    16261 cgcaagggca cggcaggctt attgctccag cgcccgcccg agcttggccc attccgaggc
    16321 cgacaggtgc gggcgcacga tctgcagcac cccctcgaag acgggagccg gcgcctggcg
    16381 gcgcatgtcg gccagcatcg cggcgcgctc ggcgggggcc atgaagggca gcatccagcg
    16441 cagcaccacc tgcatttcat cgggcggtat cgaggccacc agcgcgccct cgatcgtggc
    16501 cagttcggca tcgctgtagt gcgcccaaag cacggcgttg tgggccgtct cctcctggtg
    16561 catatgctcg aagttgtgcg ccacgaacag cgccagctgc tggtacagcg catgcgcgag
    16621 cggcgcacgc cttgtgcgcg gacaggccag caactcagcg gtggcggcgg ccagcatggc
    16681 aatcgcctgc tcgtggccca gatgttcctg cgcgatggcg gcggcgctgc cgggggcgtg
    16741 gcgctccatg gccgggtgca cgaagccgtt ctcgtgccgc aggtgcgagc ggcagaactc
    16801 cagcagctgc tgcacgcgcg cgcaggcctg tgccagctcc agatcgtcct gcgtatcgac
    16861 gcggcccagg cccacgagtg tgtcggacat gtacgcgcgc agggccttgt ggatgaaggc
    16921 gtacatgtcc atgcgcggtg tgatttcggc ctgcgccagg gcggcgattt cctgttggtt
    16981 cacttgcatc atcgattcct tgaggtcggt gcgggcacac catgagcccg cacgccaagt
    17041 ctaggaacca ggccgcagcg gcgctgctaa aaagcgctta ccggaatcct cacaatttct
    17101 taaggcgacg ctgagtcgcc ctggcgccag ccgcgggcat gtccggcgcc agggccgagc
    17161 gcagccggtc gcgcgcgctg ccgctgccgg cgcgggtttc gtccaggcgc ccggcaaggg
    17221 tgtcgatatg ctccagcatc aggcgctcgg caagcgcgtt gtccccatcg gccagcgccg
    17281 caacgatggc cgcgtggtcg tcgttggagg tctgggcctc gctttgcgac tggtacagcg
    17341 ccgacaccag cgtggtgcgc gccgacaggt cgatcagcat cgacgtgagg aagcggttgc
    17401 ccaggcattc ggccaggcag atgtgaaagt cggccagcag ccagctgcgc tgggccgcgt
    17461 cagcggcggc gatggccttg cgctcgtcgg cgacatgctg gcgcagccgg cggatgctgg
    17521 tctgcagcgg cgtgccagca tcgcgcagca tgccgggctc gatcacgcgc cgggcggcgt
    17581 aggtttccag ggcgtcatcg aatgagggtt cgatgacata ccagccaacg cgcgagcgca
    17641 cctccacgaa gccgcgcgcc tgcaattgca tcagcgcctc gcgcaccagg gtgcggctga
    17701 cgctgaacag gtctgcaatg tcctgctcac ccagtcgctc gccggggcgc agcttgccgg
    17761 caagaatgga ctcgatgatg cgctcggcaa tcctggcctt ggtgatcatg ggcggctcgt
    17821 gaggggagtt gattctagcc accgatccct gcgctcaagg ctcaggccgc cgcccctatg
    17881 gcgcgcaccc gcggtcgcag ctgcgacaac acgtaataca ccacgccccc gatcgccacg
    17941 ccgatgaacc agccaaaggt gccccaccac gcgggcagca gcgtcgtgaa gttgggcaag
    18001 atggtggaga acaccgcccc gacgccggtg gcgatcaatg cgttgacgtt ccagccaccc
    18061 tggtagcggt attcgcccag ctcctggtac agcgcctgca cattgatctg cccgcgagca
    18121 atcaggtagt agtcaacgac gatgatgccc agcagcggcc ccatggtggc gccgacggcg
    18181 ttgacgaaat gcgcggcgtc gccctcccag ggcgcgaacg gatagagcaa cagggcaatg
    18241 gcagcggcga tgtagccgcc gcgcttgaag ctgatctggc gcgggaagac gttggagatg
    18301 tcaaacgccg aggacacgaa gttggccacc acgttgatgc ccagggtcgc cacggcaaag
    18361 gtcatggccg cgagcagggc caggaaccag ctgtcgaact tggcggagat ctgctcgggg
    18421 tgcagcagca cctcgccata gacctggaag gccgcgatgg tcgtgatgcc ggcgacgaac
    18481 gagaacgcga tcaggttcac aggcagaccc cagaggttgc cgcgcgtgac cgcctcgcgg
    18541 ttcttggcgt aacgcgagaa gtcgcagaag ttcaggtaca gggcagcgaa gtaggtgacc
    18601 caggtggccc ccaccgccat cagagcccag aaggagcccg gctcgccctt gatgccggcg
    18661 tccttggtct tctcgagcag cacgtccatc gggatgccat gttcgaagga gaagccgccc
    18721 gccttgacca tcagcccgat cgccagcacc agcatcgcca cccacaccgc cgggccggcc
    18781 cagtcctgga acttgcgcat ggtctccatg ccgcgctgga tgatcaagag ctgcagggcc
    18841 cacatgatca cgtagcagat gacctccagc gtggaatggc ccagcatgtg cgagttctgg
    18901 tggaactgca gcatgccttc gctgcggatc agcagggcca ccagggcgct ggaggctgcc
    18961 gcggtctgcg cgccatacca gaagatggcc acgatggcgc gcacgatagc cggcaggttt
    19021 gcgccccaga tgccgaacga ggcccgcgcc
//
LOCUS       DAOMTQ010000046        19031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_369_length_19031_cov_19.951570, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000046 DAOMTQ010000000
VERSION     DAOMTQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 19031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19031
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_19031_cov_19.951570"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="pgk"
                     /locus_tag="PK518_06490"
     CDS             <1..525
                     /gene="pgk"
                     /locus_tag="PK518_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRM48598.1"
                     /translation="NTFMLAAGLPIGKSLAEPDLLGEAKAVIAAMAARGAEVPIPTDV
                     VVAKTFAADAPATVKKATEVADDDMILDIGPETAARLAAQLKAAGTIVWNGPVGVFEF
                     DQFAGGTQAIAQAIAESSAFSIAGGGDTLAAIAKYGITQQVGYISTGGGAFLEVLEGK
                     QLPAFEILQKRAAG"
     gene            706..1392
                     /locus_tag="PK518_06495"
     CDS             706..1392
                     /locus_tag="PK518_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48599.1"
                     /translation="MSFITTLGWSAAACTVIALAGCGSSPIPISQNFELTRQHKVRSA
                     GHWELLSRDVVAQTRTTLQKAGYAPDTPLHVVAPTQPSAFDMGFRDFLITELVQDGAT
                     VHQHPGAVLEVTYNTQLVRHNSDRPHFIPGQFTILAAGVMAAYGLRHEHLDAKLLGTL
                     GLTGAADYVNSVNSGGPTNTELILTTTVTRAGQYVARKTDVYYLENADTPLFMRSAHY
                     RAINMKVASQ"
     gene            1389..1943
                     /locus_tag="PK518_06500"
     CDS             1389..1943
                     /locus_tag="PK518_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HRM48600.1"
                     /translation="MKHALALPLVAALFAVGCAQQPQKLRVEPTYQEALNAPLLQSSR
                     DAVAQLVANLDVAATGPGPVLVATVVNVNDMSHSAPLGRTLSEQYANAMAATGFDVKE
                     IKLRGEVFVKEGAGELLLSREIKSIARSQNASMVLVGTYSPAASFTYVSMKLVRTEDS
                     RIIRGHDYALPNDRDVQRLLVQPR"
     gene            complement(2044..2922)
                     /locus_tag="PK518_06505"
     CDS             complement(2044..2922)
                     /locus_tag="PK518_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM48601.1"
                     /translation="MPSFSRRQLVVLVLLTLVWGFNWPVMKVGVEHFPPLSFRMLSLW
                     TGLPILALVMLRQGLSFRMPRAYWGRLLLLALTNMVLWHALMILAMPLLSSGRAAILG
                     YTMPIFSAVIGALAFGDRLAGRAWAGVGAALLGVLLLLWHEIGALGSRPAGVTLMLVS
                     AASWALGTQLLRRLPIPVPLLTLSFWMVAITCAALTVLAVLFERERWQPPDAQALAAI
                     AYNGLLVLGFAQTAWFFLVRTLPPVASTLSVMLIPVLGVFSGTLWLGEPLSWQDWAAV
                     GLMVLAIASVLWPGKK"
     gene            complement(2928..3896)
                     /locus_tag="PK518_06510"
     CDS             complement(2928..3896)
                     /locus_tag="PK518_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HRM48602.1"
                     /translation="MSNLVPSGALRAVLQLLALLRPASAAAYRGPVQPRVFSGPGPTA
                     GADAGCAPIPRRIWSYWNGAVLDPVVQQCMDNWRLQCPDYRIDVLNADNLERFVAPGD
                     LPPDFARLAPPKQADWLRLYLVRHHGGFWLDASIVLTQSLDWLRDLQQAQRSEFAGFF
                     LRGFTHDERWPVVESWAFGAPAHAPFVTAWQQEFHHALIERGTGDYLAALQAQPMGAA
                     VLQGIADPEYLLIHVAAQQVLRRDNGYRLALLCAEDTAYYYQHALYWKWYLLYPRLCL
                     APAQERVAPLVKLRGGERRHFSALLARHGGARRGSLWQQACTPGPV"
     gene            3987..4811
                     /gene="pyrF"
                     /locus_tag="PK518_06515"
     CDS             3987..4811
                     /gene="pyrF"
                     /locus_tag="PK518_06515"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRM48603.1"
                     /translation="MTFLDMLRDATARNDSLLCVGLDPEPTRFPDQLRGDAHRIYDFC
                     AAIVDATADLVCAFKPQIAYFAAHGAEDQLERLMQHMRANAPHVPVILDAKRGDIGST
                     AEQYAVEAFERYGADAVTLSPFMGFDSVLPYLQYQGKGAFLLCRTSNPGGDDLQAQRL
                     ASVEGQPLLYEHVARLAQGPWNKNGQLGLVVGATYPDEIARVRVIAPTLPLLIPGVGA
                     QGGDAAATVRAGLTERGTIIVNSSRAVLYASGGADYAAAARAAAQATRATLNAARS"
     gene            4859..5830
                     /locus_tag="PK518_06520"
     CDS             4859..5830
                     /locus_tag="PK518_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48604.1"
                     /translation="MPTRRHATALLACGLALLTAGPAQAQDYPAKPITLVVGYPPGGS
                     TDLMGRLVGAELSTRLGQPVVIENLGGAGGTIGAQKVASAAPDGYTLLVGANNELAIA
                     KLVTKTVKYDPASFTPIGLIGSQPMVLVAATSTGVKNASDFTQLVKKNPGKFSYGSSG
                     VGTALHLAGEMVKEQAGLFMTHIPYRGVAPLTNDLVGNNLEFGVFVLSSGLPHIKSGK
                     VVALGTTAAKRAPATPDIPALAELPQFKNVDIESWFALVGPANLPQPVVAKLKKALND
                     TLQSPDFRKKMAAAGSGVAAPDANLAQFWQGEVAKYTKIVQFAKIEE"
     gene            5827..6798
                     /locus_tag="PK518_06525"
     CDS             5827..6798
                     /locus_tag="PK518_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRM48605.1"
                     /translation="MNSSLRFDLPAPDLSAWRAGNTGTEGVWHFDSGLPGRQVLVSAL
                     IHGNELCGAWALKGLLESGLRPQAGSLTLMFCNLAAFDRFDPANHDAARFVEQDMNRQ
                     WSDERMDAADSLERRRCAALRPFVARADWLLDLHSMHEPCAPLLLTGVQPRNLALAKQ
                     MAAPEHIVIDAGHQDGTRMRDYGRFGLPDAQAGDSRSLLVECGFHGDPASRAVAQDQC
                     LRFIEASGIVDGAELARLLPGWRLTDAPRQWALQVTGPVVAKNERFTFTEAFTGLEVI
                     ARAGTVIGDNDGEPVTTPYDDCVLVMPSTRQARAGVSVVRFARRRPL"
     gene            6946..8913
                     /locus_tag="PK518_06530"
     CDS             6946..8913
                     /locus_tag="PK518_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM48606.1"
                     /translation="MRLLDHLRLSHKFFILGGLALAMMAIPAGLQLRSMGAQMQQLGT
                     QARAMPALQQLGQSVRLTQVHRGLSAGQLGGDAQLAGRRPAVRDELGRTLQATADELA
                     SVAPDQASALNQLRQDWQALQQAVGDGAVTPAQSLARHNQMVARLLALSEQLQHSSGL
                     STSERRDTQALIQAALQQLPMLTEQLGQMRAMGAGALARGELPAETRGLLRALRTRAD
                     ELDSGSQVSLQRALQHAPALQTALAQALGAEKTATADALRLVDNGLLQASELRLPAGQ
                     YFDTLTAGIDAVNALGSQATQQLLNLVQQQASAAQRALWLMGAALAVLATLAVLLAAA
                     FVRSITQPVQQAVALARAVAGGDLSGADQPHGDNEVGELIAAQQQMRARLRPIVAQVR
                     HGSEGVALASSEIAQGNHDLSGRTEAQASALEQTAASMEQLSATVQNNAHTAREASAL
                     AEHTRELARQGGANVEQVVSTMHGVHQAARQMADIIQVIDGIAFQTNLLALNAAVEAA
                     RAGEQGRGFAVVAGEVRNLAGRSGEAAKQIRGLIEASNARVSAGNQQAEAAGRTMADV
                     VASMQRLNTLVGQISTASQEQANGVAQVGEAVTSLDQVTQQNAALVEQMAAAADGLSS
                     QAQGLVQAVAVFRAGASESHAPRSELLELTY"
     gene            9085..9789
                     /locus_tag="PK518_06535"
     CDS             9085..9789
                     /locus_tag="PK518_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HRM48607.1"
                     /translation="MFTGIVQATAVIAAIEDRAGLRTFSIEFPPGFCADLAIGASVAT
                     DGVCLTVTEILSPTSARFDVMQQSLAITTLGRYAVGRRVNVERAAKEGAEIGGHPLSG
                     HVDFTARLAATRVLENNLVWRVAVPPQFARYIFAKGYIAIHGASLTVAEVNRQESWFE
                     VWLIPETRRATVFEDMAVGDLLNIEIERSTQVVVDTVREAVQDSLGRLAPVLEALLQE
                     KGLSLDDYLPRPPSSL"
     gene            9894..10379
                     /locus_tag="PK518_06540"
     CDS             9894..10379
                     /locus_tag="PK518_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM48608.1"
                     /translation="MPNRWKPNVTVAAIVEREGRFLFVEEDTADGLRLNNPAGHLDPG
                     ESPIEACVREVLEETAYDFEPQALVGVYLNRFTRTRTGDDITYLRFAFAGNVGAHHPW
                     RALDDGILRAVWLTPDELRSCRARHRSPLVLQSLEDYLAGRRAPLELIHTDASVLQPP
                     R"
     gene            complement(10410..10844)
                     /locus_tag="PK518_06545"
     CDS             complement(10410..10844)
                     /locus_tag="PK518_06545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRM48609.1"
                     /translation="MSATAPPDELQAPALARGYFGIDVPFMRHIGLTPVSLAPGLART
                     RLPLRPELANSRGHGHGGAMMSALDFTLSAAARSHDPLGLGVITIEMNTHFLATATTD
                     LLIEARILRGGARTVFCEGNVIDTAGNVVCTARAVFKLVALG"
     gene            complement(10841..11053)
                     /locus_tag="PK518_06550"
     CDS             complement(10841..11053)
                     /locus_tag="PK518_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRM48610.1"
                     /translation="MTTIASPIAGLVAAIEAAEGAAIDAGATIIIVESMKMEIPVEAE
                     AAGTVRKLLVAVGEQIAEGQAVAEIE"
     gene            complement(11065..12426)
                     /locus_tag="PK518_06555"
     CDS             complement(11065..12426)
                     /locus_tag="PK518_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRM48611.1"
                     /translation="MTEARLPRRVLVANRGAVAARVIRTLRHMGIESVAVYSEADAGL
                     PYVAAADRAVAIGPAPAQQSYLNQARLLDVARESGADAVHPGYGFLSENAGFAEAVQR
                     QGLCFIGPSPRWIRELGHKTQARAFMAGQGMPQAASSPVLASSDEALAAAARIGYPVL
                     VKPAGGGGGIGMLPARDAQALAAAWAKASGIAAKFFGSAELYLERLIEAPRHIEFQVL
                     ADRHGEVRILFERDCSVQRRHQKVLEEARPVGIPEAQLADMRLLLSGLLARIGYDVIG
                     TVEMLYTPQDGFVFLEMNTRLQVEHAVTEEITGIDIVEAQIRLAAGMHMRQVLPGPMQ
                     ASGHAIEARIYAEDPVRFLPSPGPLGRFELPTGDGIRVETGYAQGASVTPFYDPMIAK
                     LVAHGHDRRDAIARLQAALAQTRIEGVKTNIPFIQRILAHADFVAGRIDTGIAQRVLS
                     PQP"
     gene            complement(12423..14495)
                     /locus_tag="PK518_06560"
     CDS             complement(12423..14495)
                     /locus_tag="PK518_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRM48612.1"
                     /translation="MSVLHALSPRAVAILGASDNPIKAGGRPIDYMRRHGYAGRIFPI
                     NPNRSEVQGLPAYASLRALPEAPDAVVISLPSEDVAAAIDDCVAVGAKAAVIYSSGFA
                     ELGDAGRAQQADLLARARRGGLRLFGPNCQGVANFASGAILNFSTMINESPPGDGPVA
                     IVSQSGAGAAILYGGLRRAGIGVRYMVSTGNEADICAAEAVRAVVQDERIRLVVLYVE
                     TLRQSAYLAEAARLARERGVTILAVKAGRTSAGQATASSHTGALAAEDALADAFLRQH
                     GIPRLADFRELIDYAPLFLSQPAARGPRVVSISNSGATCVLAADAAEEFGLAVEQFDA
                     ATLAGLQQALPSYVTARNPIDMTTALLGKPETFGNTLRALSGSGAADLVHVGFPIGGE
                     GYDFADFAAQASRFAQDSGMPIAVSVNQDWVAQAFQAQGVPTFWSERAAMRGLALLAE
                     QAGAAAQPPAISAPKAAASTTLDEPSSLARLQAAGLPVMRHTVATSEEQVRAAFGTLS
                     APLVAKGVSADLAHKSEHGLVRLNLREADQAVQALHDFAATLRGLGAQDGGLLLGAQH
                     KADFELALGAHVDAEFGVAVMIGQGGVLVETLRDVQFLVAPFGRAQALAAIERLAIAP
                     AFAAVRGMPAVALEPLADMMVRLGDWMLSQAGQVQSVDANPVLVARGAQAPVIVDAVV
                     AMAREGAV"
     gene            complement(14509..15510)
                     /locus_tag="PK518_06565"
     CDS             complement(14509..15510)
                     /locus_tag="PK518_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48613.1"
                     /translation="MTAKHWQPTRRATLALCLAASWLAAAPAQAQADNWPAKPIRIVV
                     GFPAGSFTDTIARAVSDQLSKQLGQPVIVDNKPGANGAIGVGEVARSAPDGYTLLVTN
                     SSSITINPQIYKKITYQPKDFAPVTMLLDAPFILTANPDWASKHQIRSAQDVIAYAKR
                     NPDKISYGSAGPGNIAHLAFAQWSNRTGVKTTHVPYKSASQAQLAVLSGELDTQFDTW
                     SALPQIAASKLLPLAVTAPQRMKQLPNVPTMAEAGYADFNVTFWAGLFAPAGTPPAIV
                     DKLYEATKSMLQNEHARNVLSKQGDMVMLAPEPFGQRIAKEVADWKQVIQREAIALD"
     gene            complement(15591..17138)
                     /locus_tag="PK518_06570"
     CDS             complement(15591..17138)
                     /locus_tag="PK518_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRM48614.1"
                     /translation="MSWQPEVDEIAYRHALAAKMGGDDKVARHKAAGKLTVRERIDAC
                     TDPDSFTEVGSLTGSGQYDSLGRLMEFTPSNLVMGRARIGGRPVVVVGDDFTVRGGAN
                     DGAVGDKLIFAEKMAHDLRLPMVRLVDGTGGGGSVRNIETKGYTSVPTMKVWSQVVQN
                     LSAVPVVSLALGSVAGMGAARVASSHYSVMVKGTAQLFNAGPPVVARIGQNVTKEELG
                     GSQIHTRNGVVDDEVASEEEAFDRIRRFLSYLPSSVHELPARGEVRDDAARRDEWLLS
                     AIPRDSRKVYKIRPIVECLVDQGSFLEMGRHWGRAIVTGLARVDGWPVAVVASDPYFY
                     GGGWDGPSAEKFTRFVDLAQAFHLPVVNLVDIAGFQIGVEAEKAGIMRYGARALAAVY
                     QATVPWCSIIVRRAYGVAAAGHQHMGRFNFRYAWPSANWGSLPIEGGLEVAYKAEIEG
                     ADDPMQKRAEIEQRVRSLTSPFRTAEAFGIDEVIDPRDTRRLLCEFANLAAPLREAGA
                     PRFGLRP"
     gene            17301..18950
                     /locus_tag="PK518_06575"
     CDS             17301..18950
                     /locus_tag="PK518_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRM48615.1"
                     /translation="MQTAFLSDQRFGVLVWQRGEPTPVVRLGLARDPLLCAVVGEKRA
                     AREPEPGTLFHVDVPGGCGAGGRYAVVALGDEAVEVVLVRSADESAALLDFVASVPFA
                     FAILNMFLTNPYQAITVADHEGMMRYISPVHEKFLGLAPGAATGRRAEEVIPNSRLPQ
                     VARSGKAEIGEMQQLRPGVTRIVNRIPVFEGGVLVGAVGQVSFSGVQALNRMQQRLNQ
                     LHDEVQHYKRELHQLKRTPSLNAMVGSSAPMQRLAREIDAVAPLDVPVLILGESGTGK
                     ELVAQALHARGRDAQAPLVSLNLAALPATLIEAELFGYEAGAFTGGKRQGQPGKLEQA
                     EGGTLFLDEVADIPLEIQVKLLRVLEDRHVQRLGAREGRRVNFRIVAATHRDLRALID
                     SGRFRLDLFYRLSGVVLQVPALRQRVEDIPALMQRFAESFCERNGLALPRVAPGVMSY
                     LAQQSWPGNVRQLRQRVEEALVFCDGQLLSVENFARHQDLPARPAQPPMAPPAPAEMP
                     LRSLEHAAILDAIARHGGNKKRAAQALGISRSYLYKVLAGG"
BASE COUNT         3100 a         6627 c         6477 g         2827 t
ORIGIN      
        1 aacaccttca tgctggccgc cggcctgccc ataggcaaga gcctggccga gcccgacctg
       61 ctgggcgagg ccaaggccgt gatcgccgcc atggccgcgc gcggcgccga agtgcccatc
      121 cccaccgacg tggtggtggc caagaccttt gccgccgatg cgcccgccac ggtgaaaaaa
      181 gccacggaag tcgccgacga cgacatgatc ctggacatcg gccccgagac cgccgcccgg
      241 cttgccgccc agctcaaggc cgccggcacc atcgtctgga acggcccggt gggcgtgttc
      301 gagttcgacc agttcgccgg cggcacccag gcgatcgccc aggcgattgc cgagtccagc
      361 gccttcagca tcgccggcgg cggcgacacc ctggcggcga tcgccaagta cggcatcacc
      421 cagcaagtgg gctatatctc caccggcggc ggcgccttcc tggaggtgct ggagggcaag
      481 cagctgccgg ccttcgaaat cctacagaaa cgcgcggcgg gctgaaccag gcgcactcgc
      541 gccgcgcacc gcggcgcttg cgggcaaaac aacactcttg cccgtctgga tggttaaaag
      601 caccagcccc cttgtttttg gctgcgggtc gattcagaat cgttcctaaa tgtgtgcgaa
      661 atgaagattt gtgtcttgcg cgcagtgcca aatcaggagg gaaccatgtc attcatcaca
      721 acactcgggt ggtctgcagc agcctgcacc gtcatcgcgc tggcgggctg cggcagctcg
      781 ccgataccca tatctcagaa cttcgagctg acacgccagc acaaggtgcg ctcggccggc
      841 cactgggagc tgctctcacg cgacgtggtg gcgcaaaccc gcaccaccct gcaaaaggcg
      901 ggttacgccc cggacacacc gctgcatgta gtggcgccca cccagcccag cgcatttgac
      961 atgggttttc gtgacttcct gatcaccgag ctcgtgcaag acggcgccac ggtgcatcaa
     1021 caccccggcg cggtgctgga ggtaacctac aacacccagc tggtgcgcca caacagcgac
     1081 cggccgcatt tcatccccgg ccagttcacc atactggccg caggcgtgat ggccgcctac
     1141 ggcctgcgcc acgaacacct ggatgccaag ctgctgggca cactgggcct gaccggcgct
     1201 gccgactacg tcaacagcgt caactcgggc ggcccgacga ataccgagct catcctcacc
     1261 accaccgtga cgcgcgccgg ccagtacgtg gctcgcaaaa ccgacgtgta ctaccttgaa
     1321 aacgccgaca cacccttgtt catgcgctct gcgcactacc gcgccatcaa catgaaggtg
     1381 gcctcgcaat gaagcacgca ctcgcactcc ccctggtcgc cgccctgttc gccgtcggtt
     1441 gcgcccagca gccccagaaa ctgcgcgtcg agcccaccta ccaggaagcc ctgaacgccc
     1501 cgctgctgca aagcagccgt gatgccgtcg cccagctggt cgccaacctg gacgtggcgg
     1561 ccaccgggcc cgggccggtg ctggtggcta ccgtggtcaa cgtcaacgac atgagccact
     1621 cggcaccgct ggggcgcacc ctgtcggagc aatacgccaa cgccatggcc gccaccggct
     1681 tcgacgtcaa ggagatcaag ctgcgcggcg aggtattcgt caaggaaggc gcaggcgagc
     1741 tgctgctgtc gcgcgagatc aagagcatcg cgcgcagcca gaacgcctcc atggtgctgg
     1801 tgggcaccta ctcgcccgca gccagtttca cctacgtcag catgaagctg gtgcgcaccg
     1861 aggacagccg catcatccgc ggccacgact acgccctgcc caacgaccgc gacgtgcagc
     1921 gcctgctggt gcagccgcgc tgaagcccgc cagcgccagc cccaggcctc gctacggcgg
     1981 ggcttttact atacaaatag gagctgcaag cgctttacca acgggcgcta gcggccattt
     2041 ttattacttt ttacccggcc acagcaccga tgcgatggcc agcaccatca gccccacggc
     2101 ggcccagtcc tgccagctga gcggctcgcc cagccacagc gtgccgctga acacgcccag
     2161 caccggaatc agcatcacgc tgagcgtgga tgccacgggc ggcagggtgc gcaccaagaa
     2221 aaaccaggcc gtctgcgcaa agcccagcac cagcaggccg ttgtaggcga tggcggccag
     2281 ggcctgggcg tcgggcggct gccagcgctc acgctcgaac agcacggcca gcacggtgag
     2341 cgcggcgcag gtgatcgcca ccatccagaa cgacagcgtg agcagcggca ccggtatggg
     2401 cagccggcgc aagagctgcg tgcccagggc ccagctggcg gcggagacca gcatcagcgt
     2461 cacaccggcg gggcggctgc ccagggcgcc aatctcgtgc catagcagca gcagcacgcc
     2521 cagcaacgcc gcaccgacgc cagcccacgc gcgcccggcc agccggtcgc caaacgccag
     2581 tgcgccgatg acggccgaaa agatgggcat ggtgtagccc aggatggccg cgcggccgct
     2641 ggagagcagc ggcatggcga ggatcatgag cgcatgccac agcaccatgt tggtcagcgc
     2701 cagcagcagc aagcgccccc aataggctcg cggcatgcga aacgacaggc cctggcgcag
     2761 catcaccagg gcaagaatgg gcaggcccgt ccacaaggac agcatgcgaa acgacagcgg
     2821 cgggaaatgc tccaccccga ccttcatcac cggccagttg aagccccaga ccagggtcaa
     2881 cagcacgagg acgaccagct gacggcgcga aaaggaaggc atggcggcta caccggcccg
     2941 ggcgtgcagg cctgctgcca caggctgccc cgtcgggcac cgccgtggcg cgccaggagg
     3001 gccgagaaat gccgccgctc gccgccgcgc agcttcacca gcggcgcgac ccgctcctgc
     3061 gccggcgcca ggcataggcg cgggtagagc agataccact tccagtacag ggcgtgctgg
     3121 tagtagtagg ccgtgtcctc ggcgcacagc agcgccaggc gatagccgtt gtcacggcgc
     3181 agcacctgct gggcggccac atggatgagc aggtattcag ggtctgcaat cccctgcagc
     3241 acggccgcgc ccatcggctg cgcctgcagg gccgccaggt agtcgcccgt gccgcgttcg
     3301 atcagcgcgt ggtggaactc ctgctgccac gccgtcacga acggcgcatg cgccggggcg
     3361 ccaaaggccc agctttccac caccggccag cgttcgtcat gggtaaagcc ccgcaggaag
     3421 aaacctgcaa actcgctgcg ctgcgcctgc tgcagatcgc gcagccaatc gagtgactgg
     3481 gtgagcacga tggacgcgtc gagccagaag ccgccatggt ggcgcaccag gtacagccgc
     3541 agccagtcgg cctgcttggg cggagccagc cttgcaaagt ccggcggcag gtcgccgggg
     3601 gccacgaagc gctcgaggtt gtcggcattc agcacatcga tgcggtagtc cgggcactgc
     3661 aggcgccagt tgtccatgca ctgctgcacc acggggtcga gcacggcgcc gttccagtag
     3721 ctccagatgc gccgcggaat gggggcgcag cccgcgtcgg ccccggcggt cgggccgggc
     3781 ccgctgaaca cccggggttg caccgggccg cgatacgcag ccgcgctggc ggggcgcagc
     3841 agagccagca actggaggac ggcgcgcagc gcgccggagg gcacaaggtt ggacatgaag
     3901 acacagggag gggtggagcg gcggacaaca cagacggccg cggccagcag gcgattgtcg
     3961 cctgcgccgt cctagaatcg tcgcccatga ccttcctcga catgctacgc gacgccaccg
     4021 cgcgcaacga ctccctgctg tgcgtgggcc tggacccgga gcccacgcgc tttcccgacc
     4081 aactgcgcgg cgacgcccac cgcatctacg acttctgcgc cgccatcgtg gacgccacgg
     4141 ccgacctggt gtgcgccttc aagccgcaga tcgcctactt tgccgcgcat ggcgccgagg
     4201 accagctcga gcgcctgatg cagcacatgc gcgccaacgc cccccatgtg ccggtgatcc
     4261 tggacgccaa gcgcggcgac ataggctcca ccgccgagca gtacgcggtc gaggccttcg
     4321 aacgctacgg cgccgacgcc gtgaccctgt cgcccttcat gggctttgat tcggttttgc
     4381 cctacctgca gtaccagggc aagggcgcct ttctgctgtg ccgcacctcc aaccccggcg
     4441 gcgacgacct gcaggcccaa cggctggcca gcgtcgaagg ccagccgctg ctgtacgagc
     4501 atgtggcgcg cctggcccag ggcccctgga acaaaaacgg ccagctgggc ctggtggtgg
     4561 gcgccaccta cccggatgag attgcacgcg tacgcgtcat cgcgcccacg ctgccgctgc
     4621 tcatccccgg tgtcggcgcc cagggcggcg acgccgcggc cacggtgcgc gccggcctca
     4681 ccgagcgcgg caccatcatc gtcaactcct cacgcgccgt gctctacgcc tcgggcgggg
     4741 ccgactacgc cgccgccgcg cgcgccgccg cccaggccac gcgcgccacg ctcaacgccg
     4801 cccgcagctg atttctgtca ccaccgccca agaaacacct atgcgcccct cgcccctcat
     4861 gcccacgcgc cgccacgcca cagcactgct cgcctgcggc ttggccctgc tgacagctgg
     4921 ccccgcccag gcgcaggact acccggccaa gcccatcacc ctcgtcgtcg gctacccacc
     4981 cggcggcagc accgacctga tggggcgcct ggtgggcgcc gagctgtcca cgcgcctggg
     5041 ccagcccgtg gtaatcgaga acctgggcgg cgccggcggc accataggcg cacaaaaggt
     5101 ggccagcgcc gcgcccgacg gctacaccct gctggtgggg gccaacaacg agctggcgat
     5161 tgccaagctg gtcaccaaga ccgtcaagta cgacccggcc agcttcaccc ccatcggcct
     5221 gatcggctcg caacccatgg tgctggtggc cgccacctcc accggcgtga agaacgccag
     5281 cgacttcacc cagctggtca agaagaaccc cggcaagttc agctacggca gctcgggcgt
     5341 gggcacggcc ctgcacctgg ccggcgagat ggtcaaggag caggccggcc tgttcatgac
     5401 gcatatcccc taccgcggcg tggcgccgct gaccaacgac ctggtgggca acaacctgga
     5461 gttcggcgtc ttcgtgctct ccagcggtct gccgcacatc aagtccggca aggtggtggc
     5521 cctgggcact acggcagcca aacgcgcgcc ggccacgccc gacattccgg ccctggccga
     5581 gctgccgcag ttcaagaacg tggacatcga aagctggttc gccctggtcg ggccggccaa
     5641 cctgccccag ccggtggtcg ccaagctgaa gaaggccttg aacgacaccc tgcagtcccc
     5701 tgatttccgc aagaagatgg ccgccgccgg ctcgggcgtg gccgcgcccg atgccaacct
     5761 ggcgcagttc tggcagggcg aggtcgccaa gtacacaaaa atcgtgcaat tcgcgaagat
     5821 cgaagaatga acagcagcct gcgctttgac ctgcccgcgc ccgacctgag cgcctggcgc
     5881 gcgggcaaca ccggcaccga gggcgtctgg cattttgact cgggactgcc cgggcgccag
     5941 gtgctggtca gcgcgctgat ccacggcaac gagctgtgcg gcgcctgggc actcaagggc
     6001 ctgctggaat cgggcctgcg cccgcaggcg ggcagcctga cgctgatgtt ctgcaacctg
     6061 gcggccttcg accgcttcga cccggccaac cacgacgcgg cgcgcttcgt cgagcaggac
     6121 atgaaccgcc agtggagcga cgagcgcatg gatgccgccg acagcctgga gcggcgccgc
     6181 tgcgcggcgt tgcgcccgtt tgtcgcccgg gccgactggc tgctggacct gcactccatg
     6241 cacgagccct gcgcgccgct gctgctcacc ggcgtgcagc cgcgcaacct ggccctggcc
     6301 aagcagatgg ccgcgcccga gcacatcgtc atcgacgccg gccaccagga cggcacgcgc
     6361 atgcgcgact acgggcgctt tggcctgccc gatgcgcagg cgggcgacag ccgttcgctg
     6421 ctggtcgaat gcggctttca cggcgacccg gccagccgcg ccgtggcgca agaccaatgc
     6481 ctgcgcttca tcgaggcgtc gggcatcgtc gatggcgccg agctggcgcg cctgctgccc
     6541 ggctggcgcc tgaccgatgc gccgcgccaa tgggcgctgc aggtcaccgg cccggtggtc
     6601 gccaagaacg agcgctttac cttcaccgaa gccttcaccg gcctcgaggt gatcgcccgg
     6661 gccggcacgg tgataggcga caacgatggc gagccagtca ccacccccta cgacgactgc
     6721 gtgctggtca tgcccagcac acgccaggcg cgcgccggcg tgagcgtggt gcgctttgcc
     6781 cggcggcgtc cgctgtaggc ggcgcaagcc cggcacccgc agtgccgtgg cctgaaagtc
     6841 ggccagatgg ggccgtttca gaaaacaaca gtcggatggg gcatacccac ttttggcgat
     6901 ggctcgccta caattagaag cgattactta caaatatctg cttccatgcg attgcttgac
     6961 catctgcgcc tgtcgcacaa gtttttcatc ctgggtggcc tggcgctggc catgatggcc
     7021 attcccgccg gcctgcagtt gcgcagcatg ggcgcgcaaa tgcagcagct gggcacacag
     7081 gcgcgcgcca tgccggcgct gcagcagctg ggccagtccg tgcgcttgac acaggtgcac
     7141 cgcggcctgt cggccggcca gctcggcggc gacgcgcagc tggcgggacg ccggcccgcc
     7201 gtgcgcgacg agctcggccg caccctgcag gccacggctg acgaactggc cagcgtggcc
     7261 cccgaccagg ccagcgccct gaaccagttg cgccaggact ggcaggcact gcagcaagcc
     7321 gtgggcgacg gtgccgtcac gcccgcgcaa agcctggcgc gccacaacca gatggtggcg
     7381 cggctgctgg ccctgagcga gcagctgcag cacagcagcg gcctgagcac cagcgagcgc
     7441 cgcgacaccc aggcgctgat ccaggcggcg ctgcaacagc tgcccatgct caccgaacag
     7501 ctgggccaga tgcgcgccat gggcgccggc gcgctggcgc gcggcgagct gccagccgag
     7561 acacgcggcc tgctgcgcgc cctgcgcacg cgcgccgacg agctcgacag cggcagccag
     7621 gtcagcctgc aacgcgccct gcagcacgca cccgcactgc agaccgcact ggcacaggcg
     7681 ctgggcgccg agaagaccgc caccgccgac gccctgcgcc tggtggacaa cggcctgctc
     7741 caggccagcg agctgcgcct gcccgccggg cagtatttcg acaccctgac ggctggcatc
     7801 gacgccgtga acgcgctggg cagccaggcc acacagcagc tattgaacct ggtgcagcag
     7861 caggccagcg ccgcgcagcg cgcgctgtgg ctcatgggcg cggccctggc cgtcctggcc
     7921 acgctggcgg tattgctggc cgcggccttt gtgcgctcca tcacccagcc ggtgcagcag
     7981 gccgtggccc tggcgcgcgc cgtggccggc ggcgacctga gcggcgcaga ccagccacat
     8041 ggcgacaacg aggtgggtga gctgatcgcc gcccagcagc agatgcgcgc caggctgcgc
     8101 cccatcgtgg cccaggtgcg ccacggctcc gagggcgtgg ccctggccag cagcgagatc
     8161 gcccagggca accatgacct gtccggccgc accgaggccc aggccagcgc gctggagcag
     8221 acggcagcat ccatggagca gctcagcgcc acggtgcaaa acaacgccca caccgcccgc
     8281 gaggccagcg cgcttgccga gcacacgcgc gagctggcgc gccagggcgg cgccaatgtc
     8341 gagcaggtgg tcagcaccat gcacggcgtg caccaggcgg cgcggcagat ggccgacatc
     8401 atccaggtga tagacggcat tgccttccag accaatctgc tggcgctgaa cgccgccgtc
     8461 gaggcggcgc gcgccggcga gcaggggcgc ggctttgccg tggtggccgg cgaggtgcgc
     8521 aacctggccg ggcgcagcgg cgaggccgcc aagcagatcc gcggcctgat cgaggccagc
     8581 aacgcgcgcg tgagcgccgg caaccagcag gccgaggccg ccggccgcac catggccgac
     8641 gtggtggcca gcatgcagcg cctgaacacc ctggtcggcc agatcagcac cgccagccag
     8701 gagcaggcca acggcgtggc ccaggtcggc gaggccgtga cctcgctgga tcaggtgacg
     8761 cagcagaacg ccgccctggt cgaacagatg gccgccgccg ccgatggcct tagcagtcag
     8821 gcccagggcc tggtgcaggc ggtggcggta ttccgcgccg gggcgagtga atcgcatgcg
     8881 ccccgatcag agctgcttga actcacttat tgatagctgt tagcgcttat gaatagagcg
     8941 ctacaggctg atttgttgct tgacgacaca tctcagaggg cgagacgtaa ttgcccctgc
     9001 ccgaggggca cgtgaaaacc ggtagtggct ggcacaattc ccggctttcg caccaccctt
     9061 tcgcaccatt tataggcagc agccatgttc acaggcatcg tccaggccac ggcggtgatc
     9121 gccgccatcg aggatcgcgc cggcctgcgc accttcagca tcgagtttcc acccggcttc
     9181 tgcgccgatc tggcgattgg cgccagcgtc gccaccgacg gcgtgtgcct gacggtgacc
     9241 gagattcttt cgcccaccag tgcgcgcttt gacgtgatgc agcaaagcct ggccatcacc
     9301 acgctgggcc gctacgccgt gggccggcgc gtgaacgtgg agcgcgccgc caaggagggc
     9361 gccgagatcg gcggccaccc gctgtcgggc catgtcgatt tcaccgcccg gcttgccgcc
     9421 acgcgcgtgc tggagaacaa cctggtctgg cgcgtggccg tgccgcccca gtttgcgcgc
     9481 tacatcttcg ccaagggcta tatcgccatc cacggcgcca gcctgacggt ggccgaggtg
     9541 aaccgccagg aatcgtggtt cgaggtctgg ctgatccccg agacgcgccg cgcgacggta
     9601 ttcgaggaca tggccgtggg cgacctgctg aacatcgaga tcgagcgcag cacccaggtc
     9661 gtcgtcgata ccgtgcgcga ggcggtgcag gacagcctgg gccgcctggc cccggtgctc
     9721 gaggcactgc tccaggaaaa gggcctgtcg ctggacgact acctgccgcg cccgccatcg
     9781 tcactatgaa taatgaagct accagcgctt gatgggagca tcttgaaggc agatcacctc
     9841 ttgatgcatg cgctgacgcg aagctttcac ctcggctggg gataatgcgc gccatgccaa
     9901 accgctggaa acccaacgtc accgtggccg ccatcgtcga gcgcgagggg cgttttctgt
     9961 tcgtcgagga agacacggcg gatggcctgc ggctgaacaa ccccgcgggc catctggatc
    10021 cgggcgaatc gccgattgag gcctgcgtgc gcgaggtgct cgaggaaacc gcctacgact
    10081 tcgagcccca ggccctggtg ggcgtgtacc tgaaccgctt cacgcgcacg cgcacgggcg
    10141 atgacatcac ctatttgcgc tttgccttcg ccggcaacgt gggcgcgcac cacccctggc
    10201 gcgcgctcga cgacggcatc ctgcgtgccg tgtggctcac gcccgacgag ctgcgcagct
    10261 gccgcgcgcg ccaccgcagc ccgctggtgc tgcagtccct ggaggactac ttggccggcc
    10321 ggcgcgcgcc gctggagctc atccacacgg atgccagcgt gctgcagccg ccgcgctgat
    10381 ggcgtctggc ggcgcttcaa tgagcgggat cagcccagcg ccaccagctt gaacaccgcg
    10441 cgcgccgtgc agaccacatt gccggcggtg tcgatgacgt tgccctcgca gaacacggtg
    10501 cgcgcgccgc cgcgcaggat gcgggcctcg atcagcaggt cggtcgtggc cgtggcgaga
    10561 aagtgggtgt tcatctcgat agtgatgacg cccagtccca gcgggtcgtg tgagcgcgcg
    10621 gcggcactca gcgtgaagtc cagcgcgctc atcatcgcgc cgccatggcc atggccgcgg
    10681 ctgttggcca gttccggccg cagcggcagg cgcgtgcgcg ccaggccggg tgccaggctc
    10741 acaggcgtga ggccgatgtg gcgcatgaac ggcacgtcga tgccgaagta gccgcgcgcc
    10801 agcgcggggg cctgcagctc gtcaggcggt gcggtggcgc tcattcgatc tccgcaacgg
    10861 cctggccttc ggcgatctgc tcgcctacgg cgacgagcag cttgcgcacg gtgccggcgg
    10921 cctcggcctc gacggggatt tccatcttca tcgactcgac gatgatgatg gtggcaccgg
    10981 cgtcgatggc ggcgccttcg gcggcttcga tggcggcgac caggccggcg atggggcttg
    11041 caatggtggt catggcgggt tccttcaagg ttgggggctc agcacgcgct gggcgatgcc
    11101 ggtgtcgatg cggccagcca cgaagtcggc gtgggccagg atgcgctgga tgaacgggat
    11161 gttcgtcttc acgccctcga tgcgggtctg tgccagcgcc gcctgcaggc gcgcgatggc
    11221 gtccctgcga tcgtggccat gggccacgag cttggcgatc atggggtcgt agaacggcgt
    11281 gacggacgcg ccctgggcat agccggtctc cacgcggata ccgtcgcccg tgggcagttc
    11341 gaagcgtccc agcggccccg gcgagggcag gaagcgcacc gggtcttcgg catagatgcg
    11401 cgcctcgatg gcatggccgc tggcctgcat cgggccgggc agcacctggc gcatgtgcat
    11461 gccggcagcc aggcggatct gggcttcgac gatgtcgatg ccggtgatct cctcggtcac
    11521 tgcatgctcg acctgcaggc gggtattcat ttccaggaac acgaagccgt cttgcggtgt
    11581 gtagagcatt tccaccgtgc cgatcacgtc gtagccgatg cgcgccagta ggcccgagag
    11641 cagcaagcgc atgtcggcca gctgcgcctc gggaatgccc acggggcggg cttcctccag
    11701 caccttttgg tggcggcgct gcaccgagca gtcacgctcg aacaggatgc gcacctcgcc
    11761 gtggcggtcg gccagcacct ggaattcgat gtggcgcggc gcctcaatca gcctttccag
    11821 gtacaactcg gccgagccga agaacttggc ggcgatgccg ctggccttgg cccaggcggc
    11881 ggccagcgcc tgcgcatcgc gcgcgggcag catgccgatg ccgccaccac cgccggcggg
    11941 cttgaccagc accgggtagc cgatgcgcgc ggccgcggcc agcgcctcat cgctgctcgc
    12001 cagcaccggc gagctggcgg cctggggcat gccttgcccg gccatgaagg cgcgcgcctg
    12061 cgtcttgtgg cccagttcgc gtatccagcg cggcgatggg ccgatgaagc acaggccctg
    12121 ccgctgcacg gcctcggcga agccggcgtt ctcggacaga aaaccgtagc ccggatgcac
    12181 cgcgtcggcg ccgctctcgc gcgccacgtc gagcaggcgc gcctggttca gatagctctg
    12241 ctgggcgggc gcggggccga tggccacggc ccggtcggcg gcggccacat aaggcaggcc
    12301 ggcatcggcc tcggaataca cggccaccga ttcgataccc atgtggcgca gcgtgcggat
    12361 cacgcgcgcg gccacggcgc cccggttggc gacgagcacg cggcggggca ggcgagcctc
    12421 ggtcatacgg caccctcccg cgccatggcg acgacggcat cgacgatcac gggcgcctgc
    12481 gcgccgcgcg ccaccagcac cgggttggca tcgaccgact gcacctggcc cgcctgggac
    12541 agcatccagt cgcccaggcg caccatcatg tcggccagcg gctccaatgc caccgcgggc
    12601 atgccgcgca cggcggcgaa cgcgggcgcg atggccaggc gctcgatggc ggccagcgcc
    12661 tgcgcgcggc cgaagggcgc gaccaggaat tgcacgtccc tgagcgtctc gaccaacacg
    12721 ccgccctggc cgatcatcac ggccacaccg aattcggcat cgacatgcgc gcccagcgcc
    12781 agctcgaaat ccgccttgtg ctgcgcgccc agcagcaggc cgccgtcctg cgcgcccagg
    12841 ccgcgcagcg tggccgcgaa gtcgtgcaag gcctgcacgg cctggtccgc ctcgcgcagg
    12901 ttcagccgta ccaggccatg ctcggacttg tgcgccaggt cggccgacac gcccttggcc
    12961 accagcggcg cggacagcgt gccgaaggcg gcgcgcacct gctcctcgct ggtggcgacc
    13021 gtatggcgca tcaccggcag gccggcggct tgcagccggg ccaggctgga aggttcgtcc
    13081 agcgtggtgg atgcggctgc cttgggtgcg gaaatggccg ggggctgggc ggcagcgccc
    13141 gcctgctcgg ccagcagcgc caggccgcgc atggcggcgc gctcgctcca gaaggtaggc
    13201 acgccctggg cctggaaggc ctgggccacc cagtcctggt tcacgctgac ggcgatcggc
    13261 atgccgctgt cctgtgcgaa gcggctggcc tgggcggcga aatcggcaaa gtcgtagccc
    13321 tcgccgccga tcggaaaacc cacatgcacc aggtcggccg cgccgctgcc ggacagcgcc
    13381 cgcagcgtgt tgccgaaggt ctcgggcttg cccagcaggg ccgtggtcat gtcgatgggg
    13441 ttacgcgccg tcacgtagga cggcagggcc tgctggagcc ctgcgagcgt ggcggcgtcg
    13501 aactgctcca cggccaggcc gaactcttcg gccgcatccg ccgcgagcac gcaggtggcg
    13561 ccggaattgc tgatgctgac cacccggggg ccgcgcgcgg cgggttgcga gagaaacagc
    13621 ggcgcgtagt cgatcagctc gcggaagtcc gccaggcgcg gtatgccgtg ctggcgcaag
    13681 aaggcatcgg ccagcgcgtc ctcggccgcc agcgcgccgg tgtgcgagct ggccgtggcc
    13741 tggccggcgc tggtgcgccc ggccttgacg gccaggatgg tgacgccgcg ctcgcgcgcc
    13801 agccgcgcgg cctcggccag gtaggcactt tggcgcaagg tctcgacgta gagcacgacg
    13861 aggcggatgc gctcgtcctg caccacggcg cgcacggcct cggcggcgca gatgtcggcc
    13921 tcgttgccgg tagagaccat gtagcgcacg cctatgcccg cacggcgcag cccgccgtag
    13981 agaatggccg cgcccgcgcc gctctggctg acgatggcca cggggccgtc gcccggcggg
    14041 ctctcgttga tcatggtcga gaagttgagg atggcgccgc tggcgaagtt ggccacgccc
    14101 tggcaattgg ggccgaacag gcgcagcccg ccacgccggg cgcgggctag cagatcggcc
    14161 tgttgcgcgc ggcccgcatc gcccagttcg gcaaagccgg acgagtagat caccgccgcc
    14221 ttggcgccca cggccacgca gtcgtcgatg gcggcggcca cgtcttcgct gggcaggctg
    14281 atgaccacgg cgtcgggcgc ctcgggcagc gcccgcagcg atgcgtaggc gggcaggccc
    14341 tgcacctcgc tgcggttcgg gttgatcggg aagatgcggc ccgcgtagcc gtggcggcgc
    14401 atgtaatcga tgggccggcc accggccttg atggggttgt ccgacgcgcc gaggatggcc
    14461 acggcgcggg ggctcagtgc gtgcagcaca gacatgcctg gctccttttc aatccagcgc
    14521 gatggcttcg cgctggatca cctgcttcca gtccgccacc tccttggcga tgcgctggcc
    14581 gaagggctcg ggcgccagca tcaccatgtc gccctgtttg ctgagcacgt tgcgcgcatg
    14641 ctcgttctgg agcatgcttt tcgttgcctc gtagagcttg tccacgatgg ccggcggcgt
    14701 gccggccggc gcgaacagcc cggcccagaa ggtgacgttg aagtcggcgt agccggcctc
    14761 ggccatggtg ggcacgttgg gcagctgctt catgcgctgg ggcgcggtga cggccagcgg
    14821 cagtagcttg ctggcggcga tttgcggcag cgcgctccag gtgtcgaact gggtgtccag
    14881 ctcgccgctg agcaccgcca gctgcgcctg cgaggcgctc ttgtacggca catgcgtggt
    14941 cttgacgccg gtgcggttgc tccactgtgc aaacgccaga tgggcgatgt tgccggggcc
    15001 ggccgagccg tagctgatct tgtcggggtt gcgcttggcg taggcgatca cgtcctgcgc
    15061 gctgcggatc tggtgtttgc tggcccagtc cgggttggcg gtgaggatga agggcgcgtc
    15121 cagcagcatg gtgacgggcg cgaagtcctt aggctggtag gtgatcttct tgtagatctg
    15181 cgggttgatg gtgatgctgc tggagttggt gaccagcagc gtgtaaccat cgggcgcgga
    15241 gcgggccacc tcgcccacgc cgatggcgcc gttggcgccg ggcttgttgt ccacgatcac
    15301 tggctgaccg agctgcttgc tgagctgatc cgacacggcg cgggcaatgg tgtcggtgaa
    15361 cgagcctgcc ggaaagccca ccacgatgcg gatgggcttg gcgggccagt tgtcagcctg
    15421 ggcctgtgcg ggcgcggcgg ccagccacga tgcggccagg cacagcgcca gggtggcgcg
    15481 gcgtgtcggt tgccagtgtt ttgcggtcat tgtgtctcct tggggtttta gtggaatcgg
    15541 cctccaacgc ttgcatatca agcgctgaca gctatggttt ttggtatgaa tcaggggcgc
    15601 aggccaaagc gcggcgcccc ggcctcgcgc agcggcgcgg ccaggttggc gaactcgcac
    15661 agcagccggc gcgtgtcgcg cgggtcgatc acctcgtcga tgccgaaggc ctcggccgtg
    15721 cggaacggcg aggtcaggct gcgcacgcgc tgctcgatct cggcgcgctt ttgcatcggg
    15781 tcgtcggcgc cttcgatctc ggccttgtag gccacctcga gcccgccctc tatgggcagc
    15841 gagccccagt tggccgatgg ccaggcgtag cggaagttga agcgccccat gtgctggtga
    15901 ccggcggcgg ccacgccata ggcgcggcgg acgatgatgc tgcaccacgg caccgtggcc
    15961 tggtaaacgg cggccagtgc acgtgcgccg tagcgcatga tgccggcttt ctccgcctcg
    16021 acgccgatct ggaagccagc gatatcgact aggttcacca ccggcaggtg gaaggcctgt
    16081 gccagatcga cgaagcgcgt gaatttctcg gccgaggggc cgtcccagcc accgccgtag
    16141 aaatatgggt cgctggccac cacggccacg ggccagccgt cgacacgtgc cagtccggtg
    16201 acgatggcac ggccccagtg gcggcccatt tccaggaacg agccctgatc caccaggcat
    16261 tcgacaatgg ggcgaatttt gtagaccttg cggctgtcgc gcggaatggc cgagagcagc
    16321 cattcgtcgc ggcgcgcggc atcgtcccgc acctcgccgc gcgcgggcag ctcatgcacc
    16381 gacgatggca ggtaagacag gaaacggcgt atgcgatcga aggcttcctc ctcgctggcc
    16441 acctcgtcgt ccaccacacc gttgcgcgtg tggatttggc tgccgcccag ttcttccttg
    16501 gtcacgttct ggccgatacg cgccacgacc ggtggtccgg cgttgaacaa ctgggctgtg
    16561 cccttgacca tcaccgaata gtggctggac gccacacgcg ctgcgcccat gccggccacc
    16621 gaacccagcg ccagcgacac tacaggtacg gcggagaggt tttgcaccac ctggctccag
    16681 accttcatgg tcggcacgct ggtatagccc ttggtctcga tattacgaac cgagccgccg
    16741 ccgcccgtgc catcgacgag gcgcaccatg ggcaggcgca ggtcgtgggc catcttctcg
    16801 gcgaagatca gcttgtcgcc cacggcgccg tcgttggcgc cgccgcgcac ggtgaagtcg
    16861 tcgccaacca ccaccacggg tcgcccgcct atgcgtgcgc gccccatcac caggttggac
    16921 ggggtgaact ccatgagccg gcccaggctg tcgtactggc ccgagccggt gagcgagccc
    16981 acttctgtaa aggaatccgg gtccgtgcaa gcgtcgatgc gctcgcgcac cgtcagcttg
    17041 cccgcggcct tgtggcgcgc caccttgtcg tctccgccca tcttcgccgc cagcgcatgg
    17101 cggtatgcga tttcatccac ttcgggttgc cagctcatgt cattgcctcc tgtgtgcaag
    17161 aaaaagcaca aaccatgcca aactgcaggc tattgatttt tataaatatt ttctgacgca
    17221 ggcaataagt gtccttgtga tggacatatg cggtggacac tgaggacaat gtgtttaccg
    17281 aaaacacaca aaggagatcg atgcagacag cctttctttc cgaccagcgg tttggtgttc
    17341 ttgtctggca gcgcggcgag cccacgcccg tggtacggct cgggctggcg cgcgatccgc
    17401 tgctgtgcgc tgtcgtgggt gagaagcggg ccgcgcgcga gccggaaccg ggcacgctgt
    17461 tccacgtgga tgtgcccggt gggtgcggcg ccggcgggcg ctacgcggtg gtagcgttgg
    17521 gcgacgaggc cgtggaagtc gtcctggtgc gctcggccga cgaatcggcc gcgctgctcg
    17581 acttcgtggc gtccgtgccg tttgcattcg ccatcctgaa catgttcctc accaatccct
    17641 accaggccat caccgtggct gaccacgagg gcatgatgcg ctacatcagc cccgtgcacg
    17701 agaagttcct cgggctggcg ccgggtgcgg ccacgggccg cagggccgag gaggtgatcc
    17761 ccaactcgcg cctgccgcag gtggcccgca gcggcaaggc cgagatcggc gaaatgcagc
    17821 agctccgccc cggcgtgacg cgtatcgtca accgcattcc ggtcttcgag ggcggcgtgc
    17881 tcgtgggcgc cgtgggccag gtcagcttca gcggcgtgca ggcgctcaac cgcatgcagc
    17941 agcgcctgaa ccagctgcac gacgaggtgc agcactacaa gcgcgaactg caccagctca
    18001 agcgcacgcc atcccttaac gcgatggtgg gcagcagcgc gcccatgcag cgcctggcgc
    18061 gcgagatcga cgccgtggcg ccgctggacg tgcccgtgct catcctcggc gaaagcggca
    18121 ccggcaagga gttggtggcc caggccctgc acgcgcgcgg gcgcgatgcc caggcgccgc
    18181 tggtgagcct gaaccttgcc gcgctgcccg cgacgctgat cgaggccgag cttttcggct
    18241 acgaggccgg cgccttcacc ggcggtaaga ggcagggcca gcccggcaag ctggagcagg
    18301 ccgagggcgg cacgctgttc ctcgacgagg tggccgacat tccgttggag atccaggtca
    18361 agctgctgcg tgtgctggag gacaggcatg tgcagcgcct gggcgcacgc gaagggcggc
    18421 gcgtgaactt ccgcatcgtc gccgccacgc accgcgacct gcgcgcactg atcgacagcg
    18481 ggcgcttccg gctggatctg ttctaccgct tgagcggcgt cgtgctgcag gtacccgcgc
    18541 tgcgccagcg cgtggaagat attccggccc tgatgcagcg cttcgccgag tcgttctgtg
    18601 agcgcaatgg gctcgccctg ccgcgcgtag cgccgggcgt catgagctac cttgcgcagc
    18661 agagctggcc gggcaatgtg cgccagttgc gccagcgcgt cgaggaggcc ctggtgttct
    18721 gcgacggcca gctgctgagc gtcgagaact ttgcccgcca ccaggatctg ccggcccggc
    18781 cggcgcagcc gcccatggcg ccgcctgcgc ctgcggagat gcctttgagg agcctggagc
    18841 acgcggccat cctcgacgcc attgcgcgcc atggcggcaa caagaagcgt gcggcacagg
    18901 cgctgggcat ctcgcgctcc tacctctaca aggtattggc gggcggctga gtccagaagc
    18961 cgtcagtgcg tggcagcggc ctcactgccc cgtgatcttg cgctcgcgca cgatggcgcc
    19021 ccactggtcg t
//
LOCUS       DAOMTQ010000047        18985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_373_length_18985_cov_26.516112, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000047 DAOMTQ010000000
VERSION     DAOMTQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 18985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18985
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_18985_cov_26.516112"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PK518_06580"
     CDS             complement(<1..1073)
                     /locus_tag="PK518_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM48616.1"
                     /translation="MQDKYNHTEVERAAHAHWTASDAYRVTEDQAKPKYYACSMLPYP
                     SGKLHMGHVRNYTINDMLTRQLRMKGFNVLMPMGWDAFGLPAENAALKNKVPPAQWTY
                     DNIAYMKQQMQAMGLAIDWSREVATCDPEYYKWNQWLFLKMLDKGIAYRKTQVVNWDP
                     VDQTVLANEQVIDGKGWRTGAPVEKREIPGYYLKITDYAQELLDHVQVGGEKATLSGW
                     PDKVRLMQENWIGKSAGVRFAFTHDIQDAQGHLIQDGKMYVFTTRADTIMGVTFCAVA
                     PEHPLATHAAQGNAKLAAFIEECKKGGTTEAELALKEKEGMATGLFVTHPLSGEQIAV
                     WVGNYVLMSYGDGAVMGVPAHDER"
     gene            1235..2479
                     /locus_tag="PK518_06585"
     CDS             1235..2479
                     /locus_tag="PK518_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRM48617.1"
                     /translation="MTVLTLFLSLSPWMQATIGLACLLLLAYVVQALAQYLLLHTVPR
                     LRERMDVRWARVLWHDQVLRRLARVVPSLVVQAGIGAVPHLDPVAFAVIRNLAVALTV
                     LQLVRTLNALLNAILQEQESRPEGARTATRSIKSYVQLGKLALALIGAIVIVAVLIDR
                     SPLILLSGLGAMSAVLMLVFKDTILSFTAGVQLSSNDMLRVGDWIEMPQMGADGAVID
                     IALHTVKVQNWDKTITTIPTWRLMSESYRNWRGMSESGGRRIKRQLRIDSATVRFLSD
                     ADIDRLERIALLAPYLAAKRQAVHSANAALGEGAAVPANLRRLTNIGTFRAYVQAYLQ
                     AHPGVHQGMTLMVRMLEPTAEGVPLELYCFTATTAWVQYEAIQGDIFDHLLALLPEFD
                     LRLYQSPTGWDIRSGLQQKGLP"
     gene            complement(2604..3026)
                     /locus_tag="PK518_06590"
     CDS             complement(2604..3026)
                     /locus_tag="PK518_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRM48618.1"
                     /translation="MAFGRLERTKGAEPMSDINVTPLVDVMLVLVVIFILTAPLLASS
                     IRLQLPRAEGAQGGTPEHAVSLAVDAAGQVYLDEAPVDAATLAQRLRQIANQRPDTEV
                     QLRADRSVPYGRVVEVMDAAQAAGLTRMGFVAQPSTQK"
     gene            complement(3036..3695)
                     /locus_tag="PK518_06595"
     CDS             complement(3036..3695)
                     /locus_tag="PK518_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRM48619.1"
                     /translation="MDGLHWLRQGDAVTQATAALLLVMSVASWVVIFWKLRLMRRAHV
                     DMPRCTAAFWQAPTLATAAERLPAFDREALILPLVRAAQQVEAAQAGALASQGSLSQR
                     LTRVLRDALHGVLGRLQFGQVLLATVGSTAPFVGLLGTVWGIYHALTALTGAEQISIE
                     RLAGPVGEALIMTAAGLTVAIPAVLAYNWFGRVSARIEADLEGFARDLRELLVDTDPP
                     N"
     gene            complement(3702..4526)
                     /gene="dapB"
                     /locus_tag="PK518_06600"
     CDS             complement(3702..4526)
                     /gene="dapB"
                     /locus_tag="PK518_06600"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRM48620.1"
                     /translation="MTSTSSTAQRRVAVAGASGRMGRMLIEAICASDDCLLAGALDLA
                     GSPAVGQDAAAFLGRASGVAITADLRAGLQNADVLIDFTRPEGTLAHAAICAELGVQM
                     VIGTTGFTDAQKAELDAAARRVAMVFAPNMSVGVNVTLKLLQMAAQALDAGYDIEIIE
                     AHHKHKVDAPSGTALKMGEVIAQAQGTLLADRAVYERYGHTGERKSGSIGFATVRGGD
                     IVGDHTVLFAGTGERVEITHKSSSRAGYAQGSLRAVRYLAGQRAGMFDMFDVLNLK"
     gene            complement(4587..5147)
                     /locus_tag="PK518_06605"
     CDS             complement(4587..5147)
                     /locus_tag="PK518_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRM48621.1"
                     /translation="MYRPMFAPACWGARRGAIVLSALGLCTGLAGCAGTGERLASMVT
                     PYKVDVVQGNFISREQVQALQPGMGRQQVREILGTPLMVSLFHGDRWEYVFTLKRPDV
                     PAQTRKLTVFFKENMLDHVEGDEMPSEAEFVASLSSRRAQAKVPPLEATEAQLERFPK
                     AKPASAAPAAPTPAPAASYPPLESRR"
     gene            5246..5665
                     /gene="fur"
                     /locus_tag="PK518_06610"
     CDS             5246..5665
                     /gene="fur"
                     /locus_tag="PK518_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HRM48622.1"
                     /translation="MTNIDELKSTGLKATLPRLKILEIFQKGTQRHMTAEDVFRVLLD
                     ERSDIGLATVYRVLTQFEQAGILIRSSFESGKAIYELNEGQHHDHFVCTVCSKVEEFY
                     DPEIEKRQNLIAKDKGWVIHDHSMALYGQCADCAKKR"
     gene            complement(5666..6622)
                     /gene="hprK"
                     /locus_tag="PK518_06615"
     CDS             complement(5666..6622)
                     /gene="hprK"
                     /locus_tag="PK518_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRM48623.1"
                     /translation="MKPNVVSADALFEEFRVLLKWQWIAGLGASERRFTEVAVRAARS
                     SADLVGYLNYIHPYRTQVLGEREIAYLTNATPEDCERRIARIVTLEPPVLVLADGQQA
                     PDELVSMCERAQIPLFATHESAAFIIDVLRTYLSKHFADRTTMHGVFMDILGLGVLIT
                     GESGLGKSELGLELISRGNGLVADDAVDLYRINQTTIEGKCPELLQNLLEVRGIGLLD
                     IRAIFGESAVRRRMRLKLIVHLVRKETMERDYERMPYEPLTQDVLGVPVLKVVVQVVA
                     GRNIAVLVEAAVRNTILQLRGIDTYQEFLERHRRAMESGSGA"
     gene            complement(6648..7115)
                     /locus_tag="PK518_06620"
     CDS             complement(6648..7115)
                     /locus_tag="PK518_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM48624.1"
                     /translation="MNRLASILPAAQVLVSVDATSKKRAFEEAGLLFESQHGLSRALI
                     TDSLFARERLGSTGLGHGVAIPHGRIKGLKSPMAAVFQLAHPIGFDAPDEQPVALLIF
                     LLVPEAATQKHLEILSEIAELLSDSGLRERMKACTDAASLHAMISGWQSAQGG"
     gene            complement(7252..7602)
                     /gene="raiA"
                     /locus_tag="PK518_06625"
     CDS             complement(7252..7602)
                     /gene="raiA"
                     /locus_tag="PK518_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRM48625.1"
                     /translation="MNLTISGHHLEVTPALRSYVTTKLDRIIRHFDQVVDVKVLLTVE
                     KQKEKEKRQRAECTLHVKGNDLFVESAHFDLYAAIDELMDKLDRMVVRHKDRQQDHQA
                     GLRRATAQAAAAAS"
     gene            7907..8827
                     /locus_tag="PK518_06630"
     CDS             7907..8827
                     /locus_tag="PK518_06630"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRM48626.1"
                     /translation="MTQPSTPALHTSALTSLPLLARGKVRDNYAVGDDRILMVASDRL
                     SAFDVIMGEPIPGKGELLTRMALFWFDKLGHIVPNHLTGEAPESVVTPAEAPQVRGRS
                     MLVQRLKPLPIEAVVRGYLAGSGWKEYQQSRSVCGVPLPDGLTNAARLPHSIYTPAAK
                     AAVGEHDENITFERTVEMVGPELATQIRDISIRLYEAAAAMALEKGMIIADTKFEFGL
                     DGNGRLVLMDEVLTPDSSRYWPVEGYADALAKGENPPSYDKQFVRDWLEQAQVGGKPW
                     DKTAPAPRLPREVVENTAAKYREALQRLTA"
     gene            complement(8839..9660)
                     /locus_tag="PK518_06635"
     CDS             complement(8839..9660)
                     /locus_tag="PK518_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRM48627.1"
                     /translation="MSWRRRAWLALAALLAGCASVPPPDAFITGTVIARERLYIPPDA
                     VFDAALVDVTQVGEPPLVLARQRIAPAGPLPYELRIPYRQALIQPRGRYEVHAAVTQQ
                     GRLLLDTPGVHPVLLSPAFRHVDVILARRPLQEATLNASVPLRQTYWKLIELVDGADL
                     AAPADGADAAHLVLARDSDRAMGSGGCNRFAARYQVDGGQLRFDALVSGLRLCLQGGT
                     SELAYLERLDAVRAYWQQGRSLELRDRAGKPVLRFVAEERGEPPPDDDEPPLQPS"
     gene            complement(9657..10565)
                     /gene="trxA"
                     /locus_tag="PK518_06640"
     CDS             complement(9657..10565)
                     /gene="trxA"
                     /locus_tag="PK518_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRM48628.1"
                     /translation="MIDVTVENFEAEVVAASMTTPVLVDFWAPWCGPCKTLGPVLEKL
                     EVAYAGRFTLAKIDSDQQQQLAAMFGIRSIPTCVLMMNGQPVDGFTGALPEGQLRAFL
                     DKHLPSAEELQAEEQEEQAQEALAGGDTAGALEALQQAVLTDPANDEARFDYVKLLLQ
                     QGREDDAKVAFAPVIAKAEGSRRLGALKAWMDALDFVTAGAYGARAGAEFDAKIAANK
                     RDFEARYGRARWLMAQQRWTDAMDELLEILMRDKGWNEEAARKTYIAILELIEPPRVK
                     VAEGQIPPEDPVLASYRRRLSSVVLS"
     gene            10730..11227
                     /gene="purE"
                     /locus_tag="PK518_06645"
     CDS             10730..11227
                     /gene="purE"
                     /locus_tag="PK518_06645"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRM48629.1"
                     /translation="MNPIQIGVVMGSSSDWDTMQHAVAILEQFGVAHEAQVVSAHRMP
                     DDMFRYAESAASRGLQAIIAGAGGAAHLPGMIAAKTVVPVLGVPVASRHLQGVDSLHS
                     IVQMPKGVPVATFAIGAAGAANAALFAVALLANHDPALRQKLETFRAEQTAAARAMTL
                     PPVAA"
     gene            11224..12393
                     /locus_tag="PK518_06650"
     CDS             11224..12393
                     /locus_tag="PK518_06650"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRM48630.1"
                     /translation="MSADQDVILPGATLGVLGGGQLGRMFVQEAQAMGYFTAVLDADP
                     TSPAGLVSHHHIRTGYEDAQGLAEMARLCAAVTTEFENVPAAALDLLAQTRRVSPAGS
                     AVAIAQDRAAEKAHFVRCGVPVAPHALIESPEQPAAVPADLLPGILKTARMGYDGKGQ
                     ARVTTPQELAAAWQDLGAVPCVLEKMLPLAQECSVIVARGRDGAMVHLPVQRNLHRGG
                     ILAVTEVYEQNLPPALAAQALAAAKSVADGLHYVGVLCVEFFVLQDGSLVVNEIAPRP
                     HNSGHYSQDACDVSQFELQLRCMTGLPLTQPRLHSPTIMLNLLGDLWFAQGDAPVTPA
                     WEQVLALPGCHLRLYGKRDAKRGRKMGHLNVTGATPEAVRAVALQAAAILGIAPF"
     gene            12404..13402
                     /locus_tag="PK518_06655"
     CDS             12404..13402
                     /locus_tag="PK518_06655"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRM48631.1"
                     /translation="MIIDGKLDASVQAAAQALARGALLGLPTETVYGLAADADNDAAV
                     AQIFAAKGRPANHPLIVHVADAAAITHYAKEVPLFAQQLIDAFWPGPLTLILPRLPAA
                     ARASTGGQDSVGLRCPAHPVAHAVLAACQRLDPPVWGVAAPSANLFGKVSPTTAQHVA
                     EAFGDQLLVLDGGPCAVGIESTIIDCTRGLPVLLRPGAISRADVHRVCGLEPLSKEEL
                     PALTPRASGTLLSHYAPTARVRLMDARQLQAGLDILGADAAHMALYARTPLRSQSAKI
                     IQRRMPDDAAAAAQQLFAVLRGFDDAGVKLIWIEAPPDQPDWEGVRDRLQRAAAAG"
     gene            13447..14400
                     /locus_tag="PK518_06660"
     CDS             13447..14400
                     /locus_tag="PK518_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRM48632.1"
                     /translation="MAANWMRRSFVAAACAATALLAACGSSSIDSAIAPTRIIAFGDA
                     MADVGQKGSGYTVNDGSLFNWTQQLASHYGLVLKPVSAGGTSYAQGNARISATPDAAG
                     NAGTPTVKQQIDQFLANPQFKADDVILISAGASDIIAGMAAVQAAAQTEAQYIAATGQ
                     AGKDLAAQIGRLSDAGAKHIVVTGSYDLERSPWAKALGKQALIHQASLSFNDELKVAI
                     KDMGKTVLYVDLAYYVNLFEANPGGNGFNNGKDPVCTSVDAGPGIGIGAGQVNSALCT
                     PSTIVAGADYNRYVFADKVYLTPNAQRLFGDYAQQTLSRRW"
     gene            complement(14476..15171)
                     /locus_tag="PK518_06665"
     CDS             complement(14476..15171)
                     /locus_tag="PK518_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HRM48633.1"
                     /translation="MQRKISTALIGVALLALCGTASAQWSVRGGLTHIAPRSTATDAV
                     GPMLPGPPSGISIDVKNKATAFLSLAYGLNPNMELELALGYPPTHDVTARIAGNLPPH
                     IAAFNGQKLAEVRQIAPTLFLNYKFGDASSQWRPFVGLGLNYTNFDKRKSTQANNALN
                     GGPTDIRLTDSWGLAGQVGLSYRIDERWSVTGALATARVKSRLTTTTAGVQRNIDIRF
                     HPVVFTVHLGYTF"
     gene            complement(15198..16760)
                     /locus_tag="PK518_06670"
     CDS             complement(15198..16760)
                     /locus_tag="PK518_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM48634.1"
                     /translation="MNQWLGLTMVSAAAMLAACGGGSDLGGGTETDLSGARGSLMHNP
                     PLRVTALSAAEFKDRLAASPSGQSLLALAGAPKCGVDVHYIEYGTVGGAGEKTNASGA
                     LMVPTGGVAGCGGARPVVLYAHGTTTDRAYNIADITEPARPGASEGSLLAAMYAAQGF
                     IVVAPNYAGYDVSKLGYHPYLNADQQSKDMLDALAAARKALPRIGSQDGGKLLVAGYS
                     QGGHVAMATHRALQAAGQPVTASAPMSGPYALGAFGDAVYYGNVNLGATVFTPLLAAS
                     YQKAYGNLYAQPSDMFEAAYATGIEQLLPSATPLATLVAQGKLPQTQLFSSTPPAPAF
                     AAITPPTQPPALAPLFALGFGSNNLVKNSARLAYLQDAAANPDGAVPSVTTGAQAAAP
                     QHPLRKAFKANDLRDWKPQRPVLLCGGNADPTVFYGANTALMQNLWSNPASPLALPAG
                     LLTVLDVDSASAGAADPFAAVKAGFAQAKAGAAAQGGANAVVQAYHGSLVPPFCNVAA
                     RAFFQQVLASGG"
     gene            complement(16896..18359)
                     /gene="dacB"
                     /locus_tag="PK518_06675"
     CDS             complement(16896..18359)
                     /gene="dacB"
                     /locus_tag="PK518_06675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRM48635.1"
                     /translation="MLISPLASARRRVAGALLVLTSLTACAAGPGARLAPATDAAALP
                     PAVIAALQRARLPQDALAAVVVPVEGRAAPRLMHQADQALNPASVMKLVTTYAALDLL
                     GPAFTWDTPVYLDTTPVAGRLRGNVYIQGRGDPKLVVERLWLLMRRLRALGIDAIDGD
                     IVLDRSAFALPAHDAADFDGEPWRPYNAAPDALLLNYKAVAMGFTPDTAAGVAHVRYE
                     PPLARLQLPTSVPLAPAGTACGDWRMGLQAELTHAERIAFQGAYPAACGAREWSVAPS
                     DPSGYAARAVEGMWRELGGRLAGRVRDGQVPAGLQPAYSNPSPPLAEVVRDINKHSNN
                     VMTQQLLLTLGLQKAGQGSFDAARGVLAQWWQERLGPTDQPVVDNGAGLSRAARLSPR
                     ALARMLQQAWASPVMPEFVASLPIVGVDGTLRRRPALVSGAAHLKSGSLRDVTALAGY
                     VLAASGQRYVLVAMVNHPNAGAARPALDALIAWSAAD"
     gene            18401..>18985
                     /gene="tsaB"
                     /locus_tag="PK518_06680"
     CDS             18401..>18985
                     /gene="tsaB"
                     /locus_tag="PK518_06680"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRM48636.1"
                     /translation="MNLLAFDTSTDTLSVAVQHGAQVLEHSGPGGAQASTTLIPAIRA
                     LLAQAGLTFARLDAVVFGRGPGSFTGLRTACSVAQGLAFGARGGLGVPVLPVDSLLAV
                     AEQARAEHGCTRVLATLDARMNEVYCAPYQWLPDGGTPAGGRWSEPQDFGLCAPEAVV
                     LPAGWTLAGNARAAYGARLAPDSPTEPDVPALPTA"
BASE COUNT         2954 a         6551 c         6526 g         2954 t
ORIGIN      
        1 cgctcgtcgt gcgcgggcac gcccatcacg gcgccgtcgc cgtagctcat cagcacgtag
       61 ttgccgaccc agacggcgat ctgctcgccg ctcaaggggt gggtgacgaa caggccggtg
      121 gccatgccct ctttttcctt cagcgccagc tcggcctcgg tggtgccgcc cttcttgcat
      181 tcttcaatga aggcggccag cttggcattg ccctgcgcgg cgtgcgtggc cagcgggtgc
      241 tcgggggcca cggcgcagaa ggtcacgccc atgatggtgt cggcgcgcgt ggtgaagaca
      301 tacatcttcc cgtcctggat caaatgacct tgcgcgtcct ggatgtcgtg cgtgaaggca
      361 aagcgcacgc cggcgctctt gccgatccag ttctcctgca tcaggcgcac cttgtcgggc
      421 cagccgctca aggtggcctt ttcgccgccg acctgcacat ggtcgagcag ctcctgcgcg
      481 tagtcggtga tcttcaggta gtagccgggg atctcgcgct tttccaccgg cgcgccggtg
      541 cgccagccct tgccgtctat cacctgctcg ttggccagca cggtctggtc caccgggtcc
      601 cagttcacca cctgggtctt gcggtaggca atgcccttgt ccagcatctt caaaaacagc
      661 cactggttcc acttgtagta ctccgggtcg caggtggcga cctcgcggct ccagtcgatg
      721 gccaggccca tggcctgcat ctgctgcttc atgtaggcga tgttgtcgta ggtccactgc
      781 gcgggcggca ccttgttctt cagcgcggcg ttctcggccg gcaggccgaa ggcgtcccag
      841 cccatgggca tgagtacatt gaaccccttc atgcgcagct ggcgcgtgag catgtcgttg
      901 atggtgtagt tgcgcacatg gcccatgtgc agcttgccgc tggggtaggg cagcatggag
      961 caggcgtagt acttcggttt tgcctgatcc tcggtgacgc ggtaggcatc gctggccgtc
     1021 cagtgggcgt gcgcggcgcg ctccacctcg gtgtggttgt atttgtcttg catcgggaag
     1081 ctggataaga aaggcggcgg ggaactgcgc tgctgcgcag tggtcacaag ggtctgacaa
     1141 ttgcgcccaa ttctaggcgt tcatcccccc accaccgcgc agcgccagcc catggggccg
     1201 gcatgccgca gcattgcaag gagttttccc gcccttgacc gtcctcacgc tgtttctgtc
     1261 gctatcaccc tggatgcagg ccaccatagg cctggcctgc ctgctgctgc tggcctacgt
     1321 ggtacaggcg ctggcgcaat acctgctgct gcacacggtg ccgcgcctgc gcgagcgcat
     1381 ggatgtgcgt tgggcacgcg tgttatggca cgaccaggtg ctgcgccgcc tggcgcgcgt
     1441 ggtgccgtcg ctggtggtgc aggcgggcat tggcgccgtg ccgcacctgg acccggtggc
     1501 ctttgcggtg atccgcaacc tggccgtcgc gctgaccgtg ctgcagctgg tgcgcacgct
     1561 gaacgccttg ctcaacgcca tcctgcagga gcaggagagc aggccggagg gcgcgcgcac
     1621 ggccacccgc tccatcaaga gctatgtgca gctgggcaag ctggcgctgg cgttgatcgg
     1681 tgccatcgtc atcgtggcgg tgctgatcga ccgctcgccg ctgatcctgc tgtcgggcct
     1741 gggcgccatg tcggcggtgt tgatgctggt gttcaaggac accatcctgt cgttcaccgc
     1801 gggcgtgcag ctgtcgtcca acgacatgct gcgcgtgggc gactggatag agatgccgca
     1861 gatgggcgcc gacggcgcgg tgatcgacat cgccctgcac acggtgaagg tgcagaactg
     1921 ggacaagacg atcaccacca tccccacctg gcgcctgatg agcgagagct accgcaactg
     1981 gcgcggcatg agcgaatcgg gcgggcgccg tatcaagcgc cagctgcgca tcgacagcgc
     2041 cacggtgcgc ttcctgagcg acgcggacat cgaccgcctg gagcgcatcg ccctgctggc
     2101 gccctatctg gcggccaagc gccaggccgt gcacagcgcc aacgccgcgc tgggcgaggg
     2161 cgccgccgtg cccgccaacc tgcgccggct gaccaacatc ggcacctttc gcgcctatgt
     2221 gcaggcctac ctgcaggccc accccggcgt gcaccagggc atgacgctga tggtgcgcat
     2281 gctcgaaccc acggccgagg gcgtgccgct ggagctgtac tgctttaccg ccaccacggc
     2341 ctgggtgcag tacgaggcca tacagggcga catcttcgac cacctgctgg cgctgctgcc
     2401 cgaattcgac ctgcgcctgt accaaagccc cacgggctgg gacatccgca gcgggctgca
     2461 gcaaaagggc ttgccatgac agcgggagca ccggcgtctc gccggtacgc acgaccatgc
     2521 cggcgagacg ccggcgctcc cggagatgcc tgaattggga gttgtttggc ctataacgcc
     2581 cgtacagcaa gcgccagacg ctcttatttt tgagttgatg gctgggccac gaaacccatg
     2641 cgcgtcaggc cggccgcctg cgccgcatcc atgacctcga ccacacgccc atagggcacg
     2701 ctgcggtcgg cgcgcagctg aacctcggtg tcggggcgct ggttggcgat ctgccgcagg
     2761 cgctgggcca gggtggcggc gtccaccggc gcttcgtcca gatagacctg gccggcggca
     2821 tcgaccgcca gcgataccgc atgctccggc gtgccgccct gggcgccctc ggcgcgcggc
     2881 agttgcaggc ggatgctgct ggccagcagc ggcgcggtga ggatgaagat caccaccagc
     2941 accagcatca cgtccaccag cggggtcacg ttgatgtcgc tcatgggctc cgcgcccttg
     3001 gtgcgctcca ggcggccgaa tgccatggcg tgccgttagt tgggcgggtc ggtatcgacg
     3061 agcagctcgc gcaggtcgcg cgcgaagccc tccaggtcgg cctcgatgcg ggcgctgacg
     3121 cggccgaacc agttgtaggc cagcacggcg gggatggcca ccgtcaggcc ggctgcggtc
     3181 atgatcagcg cctcgcccac ggggccggcc aggcgctcta tgctgatctg ctcggccccg
     3241 gtgagcgccg tgagcgcgtg gtagatgccc cacaccgtgc ccagcaggcc cacgaagggc
     3301 gcggtggagc caacggtggc cagcagcacc tggccgaatt gcaaccggcc cagcacgcca
     3361 tgcagcgcat cgcgcagcac gcgcgtgagg cgctgcgaca ggctgccctg actggccagg
     3421 gcaccggcct gggcagcttc gacctgctgc gccgcgcgca ccaggggcag gatcagcgct
     3481 tcgcggtcga aggcgggcag gcgctcggcc gcggtggcca gggtgggtgc ctgccagaag
     3541 gcggccgtgc agcgcggcat gtccacatgg gcgcggcgca tcaggcgcag cttccagaag
     3601 atgacgaccc agctggccac cgacatgacc agcagcagcg ccgccgtggc ctgcgtgacc
     3661 gcatccccct ggcgcaacca gtgcaggccg tccatgtgcc cttacttcag gttcagcacg
     3721 tcgaacatgt cgaacatgcc cgcacgttgt ccggccaggt agcgcacggc gcgcaggctg
     3781 ccctgggcgt agccggcgcg gctgctggac ttgtgcgtga tctccacgcg ttcgccggtg
     3841 ccggcgaaca gcaccgtgtg gtcgcccacg atgtcgccgc cgcgcacggt ggcaaagcct
     3901 atgctgccgc tcttgcgctc accggtgtgg ccgtagcgct cgtacacggc gcggtcggcc
     3961 agcagcgtgc cctgggcctg ggcaatcacc tcgcccatct tcagtgccgt gcccgagggc
     4021 gcatccacct tgtgcttgtg gtgcgcctcg atgatctcga tgtcgtagcc cgcatccagc
     4081 gcctgcgccg ccatttgcag cagcttgagc gtgacgttca cccccacgct catgttgggc
     4141 gcgaacacca tggccacgcg gcgcgcggcg gcgtccagct cggccttctg cgcatcggtg
     4201 aaacccgtcg tgcctatgac catctgcacg cccagctcgg cacagatggc ggcatgcgcc
     4261 agcgtgccct cggggcgcgt gaagtcgatc agcacgtcgg cgttctgcag gcccgcgcgc
     4321 aggtcggcgg taatggcgac accgctggcg cggcccagga aggccgccgc gtcctggccc
     4381 acggcggggc tgccggccag atccagggct ccggccagca ggcagtcatc gctggcgcaa
     4441 atggcctcga tgagcatgcg gcccatgcgg ccggaggcgc cggcgacggc cacgcggcgc
     4501 tgtgcggtag aggaggtaga ggtcatgtcg gctcgcaatc aaaacaaatc ggactggcct
     4561 ggccaggcca gccgcgggcg cgcgactcag cggcgcgatt ccagcggcgg gtagctggcg
     4621 gcgggggcag gcgtgggcgc cgcgggggca gccgaggccg gcttggcctt ggggaagcgc
     4681 tccagctggg cctcggtggc ttcgagcggc ggcaccttgg cctgggcccg cctggagctg
     4741 agcgaggcca cgaactcggc ctcggagggc atctcgtcgc cctcgacatg atcaagcatg
     4801 ttctccttga agaacacggt gagcttgcgc gtctgcgccg gcacgtccgg gcgcttcagg
     4861 gtaaacacat attcccagcg gtcgccatgg aacaggctca ccatcaacgg cgtgcccaga
     4921 atttcgcgca cctgctggcg ccccatgccg ggctgcagcg cctgcacctg ctcgcgcgag
     4981 atgaagttgc cctgcaccac gtccaccttg tagggcgtga ccatgctggc caggcgctcg
     5041 ccggtgccgg cgcaaccggc caggccggtg cacaggccca gggccgacaa cacaatggcg
     5101 ccccggcggg cgccccaaca ggcgggagca aacatgggtc tatacatagg ggatatgatc
     5161 cgggccattg tagcggccgg ggccttgcgc acagcccgcg gggctctccc agctgatttc
     5221 gccctggcac cagaaagaac ctgtcatgac gaacatcgac gaactcaaaa gcaccggcct
     5281 caaggccacc ctgccacggc tgaagatcct ggagatcttc cagaagggca cgcagcgcca
     5341 catgacggcc gaggacgtgt tccgcgtgct gctcgacgag cgctcggaca tcggcctggc
     5401 cacggtgtac cgcgtgctca cgcagttcga gcaggccggc atcctgatcc gcagcagttt
     5461 tgagagcggc aaggcgatct acgaactcaa cgagggccag caccacgacc atttcgtgtg
     5521 cacggtctgc agcaaggtgg aagagttcta cgaccccgag atcgagaagc gccagaacct
     5581 gatcgccaag gacaagggct gggtgatcca cgaccactcg atggcgctgt acggccaatg
     5641 cgccgactgc gcgaagaaac gctgatcagg cgccgctgcc gctttccatg gcccggcggt
     5701 ggcgctccag gaactcctgg taggtgtcta tgccgcgcaa ctgcaggatg gtattgcgca
     5761 ccgccgcctc gaccagcacg gcgatgttgc gtccggccac cacctgcacc accaccttga
     5821 gcacgggcac gcccagcacg tcctgggtga gcggctcgta gggcatgcgc tcgtagtcgc
     5881 gctccatggt ctccttgcgc acgaggtgca cgatgagctt caggcgcatg cgccggcgca
     5941 cggccgattc gccaaagatg gcgcggatgt ccagcaggcc tatgccgcgc acctcgagca
     6001 ggttttgcag cagctccggg cacttgccct cgatggtggt ctggttgatg cggtacagat
     6061 ccaccgcatc gtcggccacc aggccattgc cgcgcgagat cagctccaac cccagctcgc
     6121 ttttgcccag gccggattcg cccgtgatca gcacgcccag gcccaggatg tccatgaaca
     6181 cgccatgcat ggtggtgcgg tcggcgaaat gcttggacag gtaggtgcgc agcacgtcga
     6241 tgatgaaggc cgccgactcg tgcgtggcaa acagcgggat ctgcgcacgc tcgcacatcg
     6301 acaccagctc gtccggcgcc tgctggccgt cggccagcac cagcaccggg ggctccagcg
     6361 tgacgatgcg cgcaatgcgg cgttcgcagt cctcgggcgt ggcgttggtc aggtaggcga
     6421 tctcgcgctc gcccagcacc tgcgtgcgat agggatggat gtagttcagg tagcccacca
     6481 gatcggcgct ggagcgcgcg gcgcgcacgg cgacctcggt gaagcgccgc tccgatgccc
     6541 ccaggccggc gatccactgc catttcagca ggacgcggaa ctcctcgaag agcgcatcgg
     6601 cactgacgac gttgggtttc acggcggctg gcgggcgggt gtcgggctca tccgccctgg
     6661 gcggactgcc agcctgagat catggcgtgc aggctggcgg cgtcggtgca ggccttcatg
     6721 cgctcgcgca ggccgctgtc gctgagcagc tcggcgatct cggagaggat ttccaggtgc
     6781 ttttgcgtcg ccgcctcggg caccaacaga aagatcagca gcgccacggg ttgttcgtcg
     6841 ggggcgtcaa agccgatggg atgcgccagc tggaacaccg ccgccatggg cgacttgagc
     6901 cccttgatgc ggccatgcgg aatagccacg ccgtggccca ggccggtgga gcccaggcgc
     6961 tcgcgggcaa acaggctgtc ggtgatcagg gcccgggaca ggccgtgctg gctctcaaac
     7021 agcagcccgg cctcctcgaa cgcacgcttc ttgctggtgg catcgacgct cacgagcact
     7081 tgtgcggcag gcagtatgga agctagtcga ttcatggtca gtaggtaacg attatgcacg
     7141 cctagcggaa accctgaaag tgtatctgtc ggcaacaaaa aaccgccacg ggggcggttg
     7201 ctgcgccagc cgccgcatgg gcgatggcgc gggccagggc aggcacggct gtcaggacgc
     7261 cgcggcggcc gcctgggccg tggcgcggcg caggccggcc tggtgatcct gctggcgatc
     7321 cttgtggcgc acgaccatgc ggtcgagctt gtccatcagt tcatcgatgg ccgcatacag
     7381 gtcgaaatgg gcgctctcca cgaacaggtc attgcctttc acatgcagcg tgcattccgc
     7441 gcgctggcgt ttttccttct ccttttgctt ctctacggtc agtagtacct tcacgtccac
     7501 cacttggtcg aagtggcgaa tgatgcgatc cagcttggtg gtcacatagc tacgcaaggc
     7561 cggggtcact tcgagatgat gaccgctgat cgtcaaattc atagacaagc ctccttcaat
     7621 tgaacaatgc cgcccacccg attggaagct gggcgaccgg ggatggcatg cgcacatccc
     7681 tggattcact atgcgcgtgc cggccgtgga acgcaacgcc tttcctggcc cgcggatatg
     7741 ggatatgcgg aacaaatgtc aaaccagcag aaatgttcca caatgggccg gctttcccct
     7801 gcgcgcggcg cccgcctgcg caacccgggc gcaccgctag aatgggccgc tttcgctgcg
     7861 cccgtcggcg cagccgattc gttttgcgcg cgcctgcccc accgacatga cccagcccag
     7921 cacccctgcc ctgcacacct ccgccctgac ctcgctgccg ctgctggcgc gcggcaaggt
     7981 gcgcgacaac tacgccgtgg gggacgaccg catcctgatg gtggccagcg accggctctc
     8041 ggcctttgac gtgatcatgg gcgagccgat tcccggcaag ggcgaactgc tcacgcgcat
     8101 ggcgctgttc tggtttgaca agctggggca tatcgtgccc aaccacctga cgggcgaggc
     8161 acccgagagc gtggtcacgc ccgccgaggc accccaggtg cgcggccgct ccatgctggt
     8221 gcagcgcctc aagcccctgc ccatcgaggc cgtggtgcgc ggctacctgg ccggcagcgg
     8281 ctggaaggaa taccagcagt cgcgctcggt ctgcggcgtg ccgctgccgg acggcctgac
     8341 caacgccgcc aggctgccgc acagcattta cacccccgcc gccaaggctg cggtgggcga
     8401 gcatgacgag aacatcacct tcgagcgcac ggtggagatg gtgggcccgg agctggccac
     8461 gcagatccgc gacatcagca tccgcctgta cgaggccgcg gccgcgatgg ccctggaaaa
     8521 gggcatgatc attgccgaca ccaagttcga gttcggcctg gacggcaatg gccgcctggt
     8581 gctgatggac gaagtgctga cgcccgacag ctcgcgctac tggcccgtcg agggctacgc
     8641 cgacgccctg gccaaaggcg agaacccgcc cagctacgac aagcagttcg tgcgcgactg
     8701 gctggagcag gcccaggtgg gcggcaagcc ctgggacaag accgcgcccg cgccgcgcct
     8761 gccgcgcgag gtggtggaaa acaccgccgc caagtaccgc gaggcgctac agcggctcac
     8821 cgcctgacca gggccgcatc acgagggctg cagcggcggc tcgtcgtcgt ctggcggcgg
     8881 ctcgccgcgt tcctcggcca cgaagcgcag cacgggcttg ccggcccgat cgcgcagctc
     8941 caggctgcgg ccctgctgcc agtaagcacg caccgcatcc agccgctcga ggtaggccag
     9001 ctcgctggtg ccgccctgca ggcacaggcg caggcccgac accagggcat cgaagcgcaa
     9061 ctgccccccg tccacctggt agcgcgcggc gaagcggttg cagccgcccg agcccatggc
     9121 ccggtcggag tcgcgcgcca gcaccagatg ggcggcatcg gccccgtccg ccggcgctgc
     9181 caggtcggcg ccatccacca gttcgatcag cttccagtag gtttggcgca ggggtacgct
     9241 ggcgttcagc gtggcctcct gcaagggcct gcgcgccagg atcacatcca catggcggaa
     9301 cgcggggctg agcagcaccg ggtgcacgcc cggcgtgtcc agcagcaggc gcccctgctg
     9361 cgtgaccgcg gcatgcacct catagcggcc ccgcggttgt atcagcgcct ggcggtaggg
     9421 gatgcgcaat tcatagggca gcggcccggc cggggcgatg cgctggcgcg ccagcaccag
     9481 gggcggctcg cccacctgcg tgacatccac cagggcggca tcgaacaccg cgtcaggggg
     9541 gatgtagagc cgctcgcgcg cgatgacggt gccggtgatg aaggcatcgg gcggcggtac
     9601 gctggcgcag ccggccaaca gcgccgccag cgccagccac gcgcggcggc gccagctcaa
     9661 ctcagcacga cgctggacag gcgccggcgg tagctggcca gcaccggatc ctcgggcggg
     9721 atctggccct cggcgacctt gacccggggc ggctcgatga gctccaagat ggcgatatag
     9781 gtcttgcgcg cggcctcctc gttccagccc ttgtcgcgca tcaggatttc cagcagctcg
     9841 tccatggcgt ccgtccagcg ctgctgggcc atgagccagc gggcgcggcc gtagcgcgcc
     9901 tcgaaatcac gcttgttggc ggcaatcttt gcatcaaatt cggctccagc acgcgcccca
     9961 taagcgcctg cagttacaaa atcaagagcg tccatccagg ccttgagcgc gcccaggcgg
    10021 cgcgagccct cggccttggc gatcaccggg gcgaaggcca ccttggcgtc gtcctcgcgg
    10081 ccctgctgca gcagcagttt gacgtagtca aagcgcgcct cgtcgttggc cgggtcggtc
    10141 agcacggcct gctgcagcgc ctccagcgcg cccgcggtgt cgccgccggc cagggcctcc
    10201 tgggcctgtt cctcctgctc ctcggcctgc agctcctcgg cgctgggcag gtgcttgtcc
    10261 agaaaggcgc gcagctggcc ctcgggcagg gcgccggtga agccgtccac cggctggccg
    10321 ttcatcatca gcacgcaggt gggaatgctg cggatgccga acatcgccgc cagctgctgc
    10381 tgctggtcgg agtcgatctt ggccagcgtg aagcggccgg cgtaggccac ctccagcttc
    10441 tccagcaccg ggcccagtgt cttgcacggg ccgcaccagg gcgcccagaa gtccaccagc
    10501 acgggcgtgg tcatggaggc ggcaaccacc tcggcttcaa agttttctac ggtgacatcg
    10561 atcatgtgtt gacaatcccg gtgatgcggc ggtgcgcgag ttaacaaggt cagcggcgat
    10621 tctcggacat attcccggcg ccggtgcgcc atgcccggca aacgcggccc gggcataaaa
    10681 cgtaaaatcg cgggtttccc cttgcgggcg tgacctgccc atcccttaca tgaaccccat
    10741 acagatcggc gtggtcatgg gctccagcag cgactgggac accatgcagc acgcggtagc
    10801 catcctcgag cagttcggcg tcgcgcacga ggcccaggtg gtctcggccc accgcatgcc
    10861 cgacgacatg ttccgctacg ccgagagtgc cgccagccgc ggcctgcagg ccatcatcgc
    10921 cggcgcgggc ggcgcggcgc atctgccggg catgatcgcc gccaagaccg tggtgccggt
    10981 gttgggcgtg cctgtggcca gccgccatct gcagggggtg gattcgctgc actccatcgt
    11041 gcagatgccc aagggcgtgc ccgtggccac ctttgccatt ggcgcggccg gggcggcgaa
    11101 tgcggcgctg tttgccgtgg ccctgctggc gaatcacgac ccggcgctgc gccagaagct
    11161 ggagaccttc cgcgccgagc agacggccgc cgcgcgcgcc atgacgctgc cgccggtggc
    11221 cgcatgagcg ccgatcagga cgtgatcctg cccggcgcca cgctgggcgt gctcggcggc
    11281 gggcagctcg ggcgcatgtt cgtgcaggag gcccaggcca tgggctactt caccgccgta
    11341 ctcgatgccg accccaccag ccccgccggc ctggtgagcc accaccatat ccgcaccggc
    11401 tacgaggacg cgcagggcct ggccgagatg gcgcgcctgt gcgccgccgt gaccaccgag
    11461 ttcgagaacg tgccggccgc ggcgctcgat ctgctggcgc agacgcggcg cgtgtcgccc
    11521 gcaggcagcg ccgtggccat cgcccaggat cgcgcggccg agaaggcgca ctttgtgcgc
    11581 tgcggcgtgc ccgtggcgcc gcatgcgctg atcgaatcgc ccgagcagcc ggccgccgtg
    11641 cccgcagacc tgctgcccgg catcctgaaa accgcgcgca tgggctacga cggcaagggc
    11701 caggcgcggg tgaccactcc gcaagagctg gccgccgcct ggcaagactt gggggccgtt
    11761 ccctgcgtgc tcgaaaaaat gctgccgctg gcgcaggaat gctcggtcat cgtcgcgcgc
    11821 ggccgtgacg gcgccatggt gcacctgccg gtgcagcgca acctgcaccg gggcggcatt
    11881 ctggcggtga ccgaggttta tgagcaaaat ctgcctccag cgcttgccgc ccaagcgctg
    11941 gcagctgcta aatcagtagc agatggcctg cactacgtgg gtgtgctgtg cgtcgagttt
    12001 ttcgtgctgc aggacggcag cctggtggtc aacgagatcg ccccgcgccc gcacaacagc
    12061 ggccactaca gccaggacgc ctgcgacgtc tcgcagttcg agctgcagct gcgctgcatg
    12121 accggcctgc cgctcacgca gccgcgcctg cacagcccga ccatcatgct gaacctgctg
    12181 ggcgacctgt ggtttgccca gggcgatgcg cccgtcaccc cggcctggga acaggtactg
    12241 gccttgcccg gctgccacct gcgcctgtac ggcaagcgcg acgcaaaacg cgggcgcaag
    12301 atgggccacc tgaacgtgac cggcgccacg cccgaggcgg tgcgcgccgt ggcgctgcag
    12361 gcggcggcca ttctcggcat agcgccgttc tgaggggccg cgcgtgatca tcgacggcaa
    12421 gctcgatgcc tcggtgcagg ccgccgcgca ggcgctggcg cgcggcgcgc tgctgggcct
    12481 gccgaccgag acggtgtacg gcctggcggc cgatgccgac aacgacgccg ccgtggccca
    12541 gatctttgcc gccaagggcc ggcccgccaa ccacccgctg atcgtgcatg tggccgatgc
    12601 cgcggccatc acccattacg ccaaggaggt gccgctgttc gcgcagcagc tgatagacgc
    12661 cttctggccc gggcccctca cgctgatcct gccgcgcctg cctgccgcgg cgcgcgcctc
    12721 caccggcggg caggacagcg tgggcctgcg ctgcccggcc caccccgtgg cccatgcggt
    12781 gctggcggcc tgccagcggc tcgatccgcc cgtctggggc gtggccgcgc ccagcgccaa
    12841 tctgttcggc aaggtcagcc cgaccacggc gcagcatgtg gcagaggcct ttggcgacca
    12901 gctgctggtg ctggacggcg gcccctgcgc cgtggggatc gaatcgacca tcatcgactg
    12961 cacgcgcggc ctgcccgtgc tgctgcgccc cggcgccatc tcgcgcgccg acgtgcaccg
    13021 tgtctgtggg ctggaaccac tatcaaaaga agagcttcca gcgcttaccc cacgcgcgtc
    13081 tggcacgctt ttgtctcatt acgcacccac ggcacgcgtg cgcctgatgg atgccaggca
    13141 actacaggcc ggcctggaca ttctgggcgc agacgccgcc cacatggcgc tgtatgcgcg
    13201 cacgccgctg cgctcgcagt ccgccaagat catccagcgg cgcatgcccg atgacgccgc
    13261 agccgcggcg cagcagctgt ttgccgtgct gcgcggcttc gacgatgccg gcgtcaagct
    13321 gatctggatc gaggccccgc ccgaccagcc cgactgggag ggcgtacgcg accgcctgca
    13381 acgcgccgcg gcggccggct gaacaccaag gcttaaactc tcgcactctt gaatccggag
    13441 atatacatgg cagcaaattg gatgcgccgc agctttgtgg ccgccgcctg tgcagcgacc
    13501 gccctgctgg cggcctgtgg ttccagcagc atcgactcgg cgatcgcgcc gacgcgcatc
    13561 atcgcctttg gcgacgcgat ggccgacgtg gggcaaaagg gcagcggcta caccgtcaat
    13621 gacggcagcc tcttcaactg gacccagcag ctggcctcgc actacggcct ggtgctcaag
    13681 ccggtttccg ccggcggcac cagctacgcc cagggcaatg cgcgcatcag cgccacgccc
    13741 gacgccgccg gcaatgccgg cacgccgacc gtgaagcagc agatcgacca gtttctggcc
    13801 aacccgcagt tcaaggcgga cgacgtgatc ctgatcagcg ccggcgccag cgacatcatt
    13861 gccggcatgg ccgccgtgca ggccgcggcg cagaccgagg cccagtacat cgccgccacc
    13921 ggccaggccg gcaaggatct ggcggcgcag atcggccgcc tgtcggatgc cggcgccaag
    13981 cacatcgtgg tcaccggcag ctacgacctg gagcgcagcc cctgggccaa ggccctcggc
    14041 aagcaggccc tgatccacca ggccagcctg agcttcaacg acgagctgaa ggtggccatc
    14101 aaggacatgg gcaagacggt gctctacgtg gatctggcct actacgtcaa cctgttcgag
    14161 gccaaccccg gcggcaatgg cttcaacaac ggcaaagacc ccgtctgcac ctcggtggac
    14221 gccggccccg gcattggcat tggcgcgggc caggtgaact cggccctgtg cacgcccagc
    14281 acgatagtcg cgggcgccga ctacaaccgc tatgtctttg ccgacaaggt ctacctgacg
    14341 cccaacgccc agcgcctgtt cggcgactac gcccagcaga ccctcagccg tcgctggtga
    14401 tcgccccccg ccacggcggc gcacaaaaaa agccggccct gggccggctt ttttgcgccc
    14461 gcaagggttg ggcggtcaga acgtgtaacc caggtggacg gtgaacacca cggggtggaa
    14521 gcggatgtcg atgttgcgct gcacaccggc ggtggtggtg gtaagccggc tcttcacgcg
    14581 tgccgtcgcc agggcgccgg tgaccgacca gcgctcgtcg atgcggtagc tcaggcccac
    14641 ctggcccgcc aggccccagg agtcggtcag gcggatgtcg gtggggccgc cgttgagggc
    14701 gttgttggct tgcgtggact tgcgcttgtc gaagttggtg tagttcagcc ccaggccgac
    14761 gaaggggcgc cactggctgc tggcatcgcc aaacttgtag ttgaggaaca gcgtgggcgc
    14821 gatctggcgc acctcggcca gcttctggcc attgaaggcc gcgatatgcg gcggcaggtt
    14881 gccggcgatg cgggccgtca cgtcatgcgt gggcgggtag cccagggcca gctccagctc
    14941 catgttgggg ttcaggccat aggccagcga cagaaaggcc gtggccttgt tcttcacgtc
    15001 gatgctgatg cccgacggcg gaccgggcag catggggccg acggcgtcgg tggccgtgga
    15061 gcggggtgcg atatgcgtca ggccgccgcg cacgctccat tgcgccgagg ccgtgccgca
    15121 cagcgccagc agcgccacgc cgatcagggc ggtggaaatt ttgcgttgca tgtcgtcagt
    15181 ccttcttgtc ttgttgctca accgccagat gccagcacct gctggaagaa ggcgcgcgcg
    15241 gccacattgc agaacggcgg caccagcgag ccgtggtagg cctgcaccac cgcattggcg
    15301 ccaccctggg cggcggcgcc ggccttggcc tgggcaaagc cggccttgac ggcggcgaac
    15361 gggtcggccg cgccggcgct ggccgagtcc acgtccagca ccgtcaacag gccggccggc
    15421 aaggccagcg gcgatgccgg gttggaccac aggttctgca tcagcgcggt gttggcgcca
    15481 tagaacaccg tcgggtcggc attgccgccg cacagcagca ccgggcgctg gggcttccag
    15541 tcgcgcaggt cgttggcctt gaaggccttg cgcagcggat gctggggcgc ggcggcctgg
    15601 gcgccggtgg tcacgctggg cacggccccg tcggggttgg cggcggcatc ctgcaggtag
    15661 gccaggcggg cgctgttctt gaccaggttg ttgctgccaa aacccagcgc gaacagtggc
    15721 gccagggctg gcggctgggt cggcggcgtg atggcggcga aggcaggcgc cggcggcgtg
    15781 ctgctgaaca gctgcgtctg cggcagcttg ccctgggcca ccagcgtggc cagcggcgtg
    15841 gccgatggca gcagctgctc gataccggtg gcataggccg cctcgaacat gtccgatggc
    15901 tgggcgtaga ggttgccgta ggccttctgg tagctggctg ccagcagggg cgtgaacacc
    15961 gtggcaccca ggttcacgtt gccgtaatac acggcgtcgc caaaggcgcc cagcgcgtag
    16021 gggccggaca tgggcgccga tgcggtcacc ggctgacctg ccgcctgcag cgcacggtgt
    16081 gtggccatgg cgacatggcc gccctgcgaa tagccggcga cgagcagctt gccgccgtcc
    16141 tggctgccga tgcgcggcag cgccttgcgc gcggcggcca gcgcgtcgag catgtccttg
    16201 gactgctggt cggcattgag gtaggggtgg tagccgagct tggagacgtc atagccggcg
    16261 tagttgggcg ccaccacgat gaagccctgg gccgcgtaca tcgcggccag cagcgagccc
    16321 tcggaggcgc cggggcgtgc gggctcggtg atgtcggcga tgttgtaggc gcggtcggtg
    16381 gtcgtgccat gggcatacag caccaccggg cgggcgccgc cgcagcccgc gacaccgccc
    16441 gtgggcacca tcagcgcacc cgaggcattg gttttttcgc cggcaccgcc gacggtgccg
    16501 tactcgatgt aatgcacgtc cacgccacac ttgggcgcgc ccgccagggc cagcaggctc
    16561 tggccgctgg ggctggccgc cagcctgtcc ttgaactcgg ccgccgacag cgccgtgacg
    16621 cgcagcggcg ggttgtgcat gagcgagccg cgcgcgccgc tcaggtcggt ttcggtgccg
    16681 ccaccgaggt cggatccgcc tccacaggcg gcgagcattg ccgcggcgct gaccatggtc
    16741 agcccaagcc attggttcat tgatgttctc ctaaaataaa acgaacggtc gttctttttc
    16801 aggcggcgat gttaatccgg caaccactga agtcaggggc gggtaactac caatgcgaca
    16861 cggaatttca cgcgcggcag atctgcatcc agcgttcagt cggccgcgct ccaggcaatc
    16921 agcgcatcca gcgccggcct ggccgcaccg gcgtttgggt gattcaccat ggccaccagc
    16981 acatagcgct gaccgctggc ggccaggaca tagccggcca gggccgtcac gtcgcgcagg
    17041 ctgccgctct tgagatgcgc cgcgccgctg accagggccg gccggcggcg cagcgtgcca
    17101 tccacgccga cgatgggcag cgaggccacg aactccggca tcaccggcga ggcccaggcc
    17161 tgctgcagca tgcgcgccag cgcgcgtggg ctcaggcgcg cggcgcggct caggccggcg
    17221 ccgttgtcca ccaccggctg gtcggtcggt cccaggcgct cctgccacca ttgcgcgagc
    17281 acgccacgcg cggcatcaaa gctgccctgg ccggcctttt gcagccccag cgtgagcagc
    17341 agctgctggg tcatgacgtt gttgctgtgc ttgttgatgt cgcgcaccac ctcggccagc
    17401 ggcggtgatg ggttgctgta cgccggctgc agccctgcgg gcacctggcc gtcgcgcacg
    17461 cggcccgcga gcctgccgcc cagctcgcgc cacatgccct ccacggcgcg cgccgcatag
    17521 ccgctggggt cggacggcgc cacggaccat tcgcgcgcgc cgcaggccgc cgggtaggcg
    17581 ccctggaagg cgatgcgttc ggcatgggtc agttcggcct gcaggcccat gcgccagtcg
    17641 ccgcaggccg taccggcggg cgccagcggc acgctggtgg gaagctgcag gcgcgccagg
    17701 ggcggctcgt agcgcacatg ggccacgccg gcggcggtat cgggcgtgaa gcccatggcc
    17761 acggccttgt agttcagcag cagggcgtcg ggcgcggcgt tgtagggacg ccagggctcg
    17821 ccgtcaaaat cggccgcatc gtgcgcgggc agggcaaagg cactgcggtc gagcacgatg
    17881 tcgccgtcga tggcatcgat gcccagcgcg cgcaggcggc gcatgagcag ccacaggcgc
    17941 tcgaccacca gcttggggtc accgcggccc tggatgtaga cgttgccgcg cagccgccca
    18001 gccacgggcg ttgtgtccag atacaccggc gtgtcccagg tgaaggccgg gccgagcagg
    18061 tccagcgccg cataggtggt gaccagcttc atcaccgagg ccgggttcag cgcctgatcc
    18121 gcctggtgca tcaggcgcgg tgcggcgcgc ccctcgaccg gcaccaccac ggccgccagc
    18181 gcatcctggg gcaagcgggc acgctgcagc gccgcgatca ccgcgggcgg cagggcagcg
    18241 gcatccgttg ccggcgccag cctggcgcca gggccggcgg cgcaggccgt caggctggtg
    18301 agcaccaaca gggcgccggc cacgcggcgc cgggccgatg cgagggggga aatcaacatg
    18361 ccagccagtc tatcgcccac catcgataat cacgcccgcc atgaacctgc tcgccttcga
    18421 caccagcacc gacaccctgt ccgtcgccgt acagcacggc gcgcaggtgc ttgagcacag
    18481 cggccccggt ggcgcccagg catcgaccac gctgatcccc gccatacgcg cactgctggc
    18541 gcaggccggg ctcacgtttg cgcggctcga tgccgtggtc ttcggccgcg ggccgggctc
    18601 gttcaccggg ctgcgcacgg cctgctccgt ggcccagggc ctggcctttg gcgcacgcgg
    18661 cggcctgggg gtgccggtgc tgccggtgga cagcctgctg gccgtggccg agcaggcgcg
    18721 cgccgagcat ggctgcacgc gggtgctggc cacgctggac gcgcgcatga acgaggtcta
    18781 ctgcgctccc taccaatggc tgcccgacgg cggcacgccg gcaggcgggc gctggagcga
    18841 accgcaggat tttggcctgt gcgcgcccga ggccgtggtg ctgcccgctg gctggacgct
    18901 ggccggcaac gcccgcgccg cctatggcgc gcgcctggcg cccgactcgc ccaccgagcc
    18961 cgatgtgccc gccctgccca cggcc
//
LOCUS       DAOMTQ010000048        18769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_388_length_18769_cov_19.421877, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000048 DAOMTQ010000000
VERSION     DAOMTQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 18769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18769
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_18769_cov_19.421877"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK518_06685"
     CDS             <1..224
                     /locus_tag="PK518_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRM48637.1"
                     /translation="AWLRGPLKDRVRSALQGERMRDCGYFNPATISRLIDEHNSGMRD
                     HATALWMLLMFEAFLRVNEGALVGAAALH"
     gene            248..2359
                     /locus_tag="PK518_06690"
     CDS             248..2359
                     /locus_tag="PK518_06690"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRM48638.1"
                     /translation="MSPKKVLLVGAGNIARSHAAALKDTPGVVLYGVFDANAATAEGL
                     ARDFHIPNVFDSLEQAAASEADTVHVLTPPDLHLATAMPFVQAGKTVLLEKPLGVSSA
                     ECAALRCAAAASGAVVGVNQNFVFNPAYEQLKKTIASGALGKPRYLSYVYEVPLRQLT
                     ARQFSHWMFREPVNILLEQAVHPLSQVVDMAGPVRELSVLSEAPIEISPGVGLHAACQ
                     ASFACERMPAHMRFHVGSQFTVCRLTVVCDDGVAIADMFANQFHTLARTAYMDPVDAW
                     LSARSTAGQIRRQGFAVLRDYVLAMAKLKPRSDAFYVGMKNSIQAFYRELQSNGRPRI
                     DLEFGASLVEVCEQIAQGFKPLPAPAQAVAMPPANQGELVVLLGGTGFIGSYTTEALL
                     AQGYRVRVMARGARNLQAVFNHPAVEVVRGDVKSREDLERAMAGADYVINLAHGGGGA
                     DFAAIRAAMVDTAVQVAEVAHAAGARRLVHVGSIAGLYLGDAGETVRDDTPPDPLPQT
                     RNDYAHAKALADAAVLDVHRRLGLPVVLLRPGLVVGAGTSPFHGGLGFFNNDQYCVGW
                     NDGRNALPWVLVTDCANAIVGAMTSEQAVGRAYNLVGDVRPTAREYLADVAQVTGRPL
                     KFVPSSPVALWLAEMGKWLVKRATGRRVQRPYLRDIRSRGISAMFDCSAAKRDLDWAP
                     VADAKTFRQMAVMVHAEEPVA"
     gene            2383..3489
                     /locus_tag="PK518_06695"
     CDS             2383..3489
                     /locus_tag="PK518_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM48639.1"
                     /translation="MLHAFSTFQLGGPQARFVELANALGPMFRHTVVAMDNRFDAGER
                     LHPSVNWAPMTLKVIKGGALANRASFRQVLRSLQPDLLMSYNWGAIEWAAANLPQVCH
                     QVHVEDGFGPEEAARQLPRRVWMRRALLGWARVPVVVISRQLERIALQEWRLPEHRVH
                     FIANGVNVPARMRPQRELVAGAAIHVGTVAGLRSEKNIARLIKAFARLRSVQPARLTI
                     VGGGLLQAELQQLAQSLGVGDDVEFTGYLSNPGEKLKDFDLFALSSDTEQQPIALLEA
                     MAMGMPVVATAVGDVPAILADVSPDNVCPPDDAAFAQLLLQVAQANSKWSAWSSRGHA
                     LVSSTYAKPAMLAQWQAVWAGQLAGVRATSLSHA"
     gene            3493..4710
                     /locus_tag="PK518_06700"
     CDS             3493..4710
                     /locus_tag="PK518_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HRM48640.1"
                     /translation="MRILHVLDHSIPLHSGYTFRTAALLREQRALGWQTFHLTSPKQG
                     VTEQALETVDDLSFYRTAAATGAASGLPLLGQWQLMGQLTRRLREVAQEVRPDIIHAH
                     SPVLNAVPAIRVGRELGLPVVYEIRAFWEDAAVDHGTTREGSARYRLTRALETWAIRK
                     ADHVFTICEGLRSDIAGRGVDPAKVTVIPNAVDTQAFQASQAPDPALKRSLNLDGKLV
                     LGFVGSFYAYEGLDLLIQALPAILRANPAVALLLVGGGPEDARLREQVSRLQLDAHVV
                     FTGRVPHKQVSRYYDLIDVLAYPRHSMRLTELVTPLKPLEAMAQQKLFVASAVGGHKE
                     LVEHMKTGVLFEAGNKDALAQALLDLLNHRQAWPALKANGRAFVESVRNWRNSVANYK
                     PVYERITQKKVQA"
     gene            4707..5846
                     /gene="wecB"
                     /locus_tag="PK518_06705"
     CDS             4707..5846
                     /gene="wecB"
                     /locus_tag="PK518_06705"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRM48641.1"
                     /translation="MKVMVVFGTRPEAIKMAPLVKALQQAAPALQTVVCVTAQHREML
                     DQVLRLFDLQPEYDLNVMKPGQDLFDITTQILTGLKPVLAAEQPDLVLVHGDTSTTLA
                     ASLAAYYTRAKVGHVEAGLRTNNKWAPFPEEMNRRLTGAMTDVHFAPTATAKANLLRE
                     GVSADAIHVTGNTVIDALLAVVDKLRADAELRQRLDKEFSFLDPNRRLILVTGHRREN
                     FGEGFQNICHALADIASAHPDVQVLYPVHLNPNVRQPVHDILAARQLDNVHLIDPVDY
                     LPFVYLMDRAHLIITDSGGVQEEAPSLGKPVLVMRETTERPEAVEAGTVRLVGTNRQK
                     LVAEAERLLADPAAYAAMAQAHNPYGDGQASQRIVKTLLNLKNKE"
     gene            5851..7071
                     /gene="wecC"
                     /locus_tag="PK518_06710"
     CDS             5851..7071
                     /gene="wecC"
                     /locus_tag="PK518_06710"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HRM48642.1"
                     /translation="MARELQKIIVMGLGYIGLPTASMLATKGHQVLGVDVNAQAVDII
                     NSGRIHIVEPDLDILVKSAVNSGNLKASLTPEEGDTFILAVPTPFKEVDGNPKAPDLA
                     YIETATRAIAPYLREDNLVILESTSPVGTTERVQEIIVAMRPELAGKIHTAHCPERVL
                     PGQILRELVDNDRIIGGTTKAAVAKAKALYKTFCNGAILETDSRTAEMSKLVENSFRD
                     VNIAFANELSVICDHLGINVWEAIALANRHPRVNILQPGPGVGGHCIAVDPWFIVASA
                     PAQSRLIRTAREVNDAKPDWVIAKVKAKAARFHEPVIGCLGLSFKANIDDLRESPSMD
                     IVHHLHQSGIGKIMACDPNVRKEKAPFALYDLKTVIKEADILLLLVDHEEFKDIDPQV
                     IKDKVVIDTKGVLR"
     gene            7115..8311
                     /locus_tag="PK518_06715"
     CDS             7115..8311
                     /locus_tag="PK518_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRM48643.1"
                     /translation="MKILLFSTLYPSAVRPIHGIFVETRLRELLKTGEVQAKVVAPVP
                     WFPFKAARFGSYAKFAATPRMEQRNGVEVHHPRYLLPPKVGMNIAPYALALGALPTLR
                     RLQRNGFDFDLIDAHYYYPDGVAAALLAKWLGKPFVVTARGSDLNLISQYPRPRKLIL
                     QAAEQAASSIGVCQALMDKLAELGANPSKLNVLRNGVDLERFVPEDRQAARERLGLPK
                     EGSYLLMVANLVELKGHHIAIQALAHLPGVTLLIAGAGPEQAALKALAARTGVADRVH
                     WAGLVPQDELKWWYSAANALALCSSREGWPNVLLEAMACGTPVLSTNVSGTPDMVNSP
                     VAGELLESRSAQALVKAWTQLQARSPARAATRAHAETFSWMATTQGQLQLFGRVRHAQ
                     ACVDHR"
     gene            8652..9977
                     /locus_tag="PK518_06720"
     CDS             8652..9977
                     /locus_tag="PK518_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HRM48644.1"
                     /translation="MRDLIFALMLLVALPLGMARPFNAYLLWGWTAMLAPTTYFYGFM
                     VGARVNLVCALLTLLLLALGRVPWRNYQNNAVTWLYVLFAIHATLAYWFAYGDNPNND
                     QYYELLLKGLLFAVVMPFFVRERVHFHAMLIVIALGLGVHGVLNGLKTVVSAGGHLML
                     GPQGTMLNDRNHLSVALALVLPLLFYLHSYTVNRLIRLGYLGAFCVLVLAILGGGSRG
                     GFIAVSVVGAWWILTTRRKGLAVVLVMAAVMGFLAFAPENITNRMSTIEDMNDDQSFM
                     GRVFAWRVSSAIALANPVFGGGFHAVQVQWIWDSFKADPGLLGFLQLPVPTFSAKAAH
                     SIYFEVMGDLGFVGLGLFLLILLRALWSRQAIKRVTTRLGAPYQWAGDMADMLMLAVL
                     AFMVGGAAVSLGYYEVIYMVVMLMELLRLHVGRARADTVGQALPRKGAM"
     gene            9974..10936
                     /locus_tag="PK518_06725"
     CDS             9974..10936
                     /locus_tag="PK518_06725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM48645.1"
                     /translation="MISRLVLQSMSKGKLSVFLFHKVPRQRDPLIPAEVDIARFEHLL
                     DTTFTKLQVLPLGDALALLQAGRLPRRAACITFDDGYPDWLSGVAPLLLRRNLHATFF
                     ITSGQFDGVPLWHERILAAVRRLPGSVLDMGIPFLPARPVATMADRQRQVLRLEQELK
                     YLTLFRREQILQQLESMAGVQPAQVPVMSEAQLRDLHSQGFGIGAHTALHPILDYCNA
                     EEVEHEIGGARERLRAIVGGAVDGFAYPNGRPYADFSRLHVDAVKRAGYRYAVTTHWG
                     VADTSTSPFQIPRFTPWAERDWHMTFQLARNLITKPLQVPESTA"
     gene            10933..11943
                     /locus_tag="PK518_06730"
     CDS             10933..11943
                     /locus_tag="PK518_06730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM48646.1"
                     /translation="MTVTTTVKDVLRTALYRCGLLGGLHRWRNRHILTVLMFHRVLPA
                     DDPALAQADREFTFTREGFGRTLDFVQRHYNVISLDTLQAARQGRSKLPPTPLLITFD
                     DGWRDTLTHALPELERRSLPAVLFLASEVLALKEPRWWQDALISALQQPGALVRLSQA
                     AGWTDRVQGDVSQALTAHLAAMPELQRWIWLQTHAPDVATHVMDRQMVTLDELQAING
                     SSLAMGGHGHTHGPLTRSTNPQAELQASHAVLAVLGQRVHSMSFPHGAWTHELVVQAR
                     MVGFEWVFTSEPTLADASCLSDSLPALGRIHVPENTWTCKNGCIAPERLATFLFFRTV
                     QQ"
     gene            12312..13334
                     /locus_tag="PK518_06735"
     CDS             12312..13334
                     /locus_tag="PK518_06735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM48647.1"
                     /translation="MRIQRKTFCALSHAELAAWSRLLRDSTSTRWAFLSPVYAAAVHE
                     CIGTVEVLLCWRGDELAGVMLLQRARGWLGRMGLREPAGGQMSDYFGLLASPGVQLQW
                     SVLLNAAGLHCLYFTHLDETQAAHGLVGQECRIGLRTRIHPDGGAAHWEYLRTQDKKL
                     VSDTERRERKLAAEHGALVFEMQTPTPQQDLDWLVTLKNAQYQRTGHDGGALLDPANA
                     RLLQRLLTSSDAYCQPRLSVLRSGGQMVAAHFGLQCGAVLHYWFPVYDQRFAVFSPGR
                     ILFRHILAVSDLHGIACIDRGEGDSAAKRDFANEEHVFCKGIVTAGLWGQALRGVQSA
                     CWRLTA"
     gene            complement(13335..14312)
                     /locus_tag="PK518_06740"
     CDS             complement(13335..14312)
                     /locus_tag="PK518_06740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM48648.1"
                     /translation="MKQSPPPPRETPRVSVIMPVRNGKDYLQEALVSVLQQSFTELEL
                     LLVNDGSTDDDYDRYALQDARVRVLHLSGLGVSCARNVGMAQARGEFIAFLDADDIWF
                     PGKLQTQVTYFDQHPDVGVVFGQFIRWPALANGSFAPAASLVQDASQFTRMDPERSGW
                     LYTRLLDGLLVGMNTAVVRRGVYEAIGGFNEAMRQGEDYDFWLKASRVAEMHSLNGPV
                     ALYRIHGASAMHRLSAESHLVNLLKAASMRWGLKTLGGQSLGSGAFQKRLTRVHFDHG
                     YAHYWRGDRTIARAAFWQALRNGHRPLRSLVYICLSYWRAKAPGQPPAT"
     gene            complement(14309..15757)
                     /locus_tag="PK518_06745"
     CDS             complement(14309..15757)
                     /locus_tag="PK518_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRM48649.1"
                     /translation="MPSVRQKVALQFFFSNLATAANFALSIVLARLLTPQEIGIFSMS
                     AVLIAVAHVFRDFGVTAFIKREKELRPEMLRNALGVLLATSWTVAALMFLSARYWAHF
                     FHEDQVVPVVQVLALGFIFIPFGAIPMAILTRELAVEKTAYVTAASTVVYFITSILLA
                     LNGFGAMTMAWANLVNIIVAGAMARWMVGRPLPWLPGLGQVRDIVNFGLGNLFTALLK
                     AADNALPDVMLGRWMTPAAVGLFSRANSTVNMANAALLPTVNYFALPHLAKVHHAQGP
                     VGGEYLRASSLINAMMLPAFAAIAVLAHDIVSLLYGQAWMAAAPAIPWLCLSYAVSSL
                     FTLSTPALTGVGKPYAAIGPNTVVLLAKALFALWLMNGSLHNFALAMALGQLLGIPFY
                     LWIHKRHLNLTWTAWGRSTVPLVAQGLLVGGICLATRQAVPQDIAPWVAILVTGACAA
                     LAFLVGCFALSLPMAGELRRMRKALAQRRQCP"
     gene            15914..17191
                     /gene="tviB"
                     /locus_tag="PK518_06750"
     CDS             15914..17191
                     /gene="tviB"
                     /locus_tag="PK518_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HRM48650.1"
                     /translation="MNFSDSTIAIIGLGYVGLPLAVEFGKHRPVLGFDINQARVEELQ
                     AGRDHTLECSAEELRAARHLRYSHQLQDLKACQVFIVTVPTPVDQANRPDLTPLIKAS
                     QTVGAALKRGDVVVYESTVFPGATEEVCVPELERASGLKFNQDFFCGYSPERINPGDK
                     EHRLPSIKKVTSGSTPEVADAVNALYAQIITAGTHKASSIKVAEAAKVIENTQRDINI
                     ALMNELSLIFARLGIDTLEVLQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEVG
                     YHPDVILAGRRVNDNMAAHVADATIKLMLRKGIPVLGSKVLVLGLTFKENCPDVRNTK
                     VVDIVRTLQRYNAQVDVFDPWIDAREAEHEYGLQCLKAIPGPGQYAAIILAVGHRQFV
                     ELGESGIKALGESGAVLFDVKSILPLGAADGRL"
     gene            17194..18276
                     /locus_tag="PK518_06755"
     CDS             17194..18276
                     /locus_tag="PK518_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM48651.1"
                     /translation="MSTRYQAVQTQLQAESKTWLITGVAGFIGSNLLETLLKLDQRVV
                     GLDNFATGHRRNLDEVQTLVSPAQWANFQFIEGDIRILEDCRRALEFLPSPMGEGRGG
                     GQPNPIPVDYVLHQAALGSVPRSIEDPITTNNANITGFLNMLVAARDAQVQSFTYAAS
                     SSTYGDHPGLPKVEDVIGKPLSPYAVTKYVNELYADVFARTYGFQTIGLRYFNVFGPR
                     QDPDGAYAAVIPKWTAAMIQGQDVFINGDGETSRDFCFVDNAVQANLLAATVKDCSAR
                     NQVYNVAISGRTTLNGLFEELKFVLATNGTYYTKKPIYRDFRSGDVRHSQADIGKAQY
                     LLGYVPSHCIQGGIIKAMPWYIRFFM"
     gene            18310..>18769
                     /locus_tag="PK518_06760"
     CDS             18310..>18769
                     /locus_tag="PK518_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48652.1"
                     /translation="MGLSLMLYLESKSAWCKDIAIVLGVVALVSVAEIFLVNRKYGIF
                     SGGFGQSRVLDSASDVLVFAVAYGIAQLFLAMIVWRISVSLTKKQPNWAAAFLLLVLM
                     GGGFVLALSVQYKLHSYFSDAVSFALISRPCKTQPAPINTGFALFPRQLHP"
BASE COUNT         3612 a         5552 c         5775 g         3830 t
ORIGIN      
        1 ccgcatggct gcgcggcccc ttgaaagacc gcgtgcgttc ggcattgcag ggcgagcgca
       61 tgcgcgattg tggttacttc aaccccgcaa ccatcagccg cctgatcgac gagcacaaca
      121 gcggcatgcg tgaccacgcc actgcgctgt ggatgctgct gatgttcgag gcgtttctgc
      181 gcgtgaatga aggcgcgttg gttggtgccg ccgcattgca ttgaattgac atttgaggaa
      241 cgactgagtg agccctaaga aagtactgct ggttggtgcc ggcaacattg caagatccca
      301 cgccgccgca ttgaaagata cgcctggagt ggtgctgtac ggcgtgtttg acgccaacgc
      361 tgccactgcc gagggactgg cgcgcgactt ccatattcca aacgtctttg actcgctgga
      421 gcaggcagca gccagtgaag cggatacggt gcatgtcctg acgccacccg atctgcacct
      481 ggccacggcc atgccgtttg tccaggccgg caagaccgtg ttgcttgaaa agccgctggg
      541 cgtgagcagc gccgaatgtg ccgcgctgcg ctgcgccgcc gccgcctccg gggcagtggt
      601 gggggtcaat cagaactttg ttttcaaccc agcctacgaa cagctcaaaa aaaccatagc
      661 gtctggcgca ctgggtaagc cgcgttatct cagttatgtc tatgaagtgc cactgcgcca
      721 gttgaccgcg cgccagttca gccactggat gttccgcgag ccggtcaaca tccttttgga
      781 gcaggctgtc catcccttgt cgcaggtggt ggacatggca ggcccagtgc gcgagctgtc
      841 ggtattgagc gaggcaccca tcgagatttc gccaggcgtc ggcctccatg ctgcctgcca
      901 ggccagcttt gcctgcgaac gcatgccggc acatatgcgt ttccatgtgg gctctcagtt
      961 cacggtgtgc cgtctcaccg tggtgtgcga tgacggtgtt gccattgccg acatgtttgc
     1021 caaccagttc cacacattgg cgcgcacggc ctatatggat ccggtggacg catggctgtc
     1081 ggcacgctcc acggcaggcc agattcgccg gcaaggtttt gccgtgctgc gtgactatgt
     1141 cctggccatg gccaaactca agccacggtc ggacgcgttc tacgtgggca tgaaaaacag
     1201 catccaggcc ttttaccgcg agctgcagag caatggccgc ccgcgtatcg acctggagtt
     1261 tggtgcttcg ttggtagagg tttgcgagca gattgcccaa gggttcaagc ccctgcccgc
     1321 gccggcgcag gccgtggcca tgccccccgc gaaccagggc gaactggtcg tgttgctcgg
     1381 cggcaccggg tttattggct cttacaccac cgaggcgttg ctggcacaag gctatcgtgt
     1441 acgcgtcatg gcccgggggg cgcgcaacct gcaagcggtg ttcaaccacc cggcggtaga
     1501 ggtggttcga ggcgacgtca agagccgcga agacctggag cgcgccatgg ctggcgccga
     1561 ctatgtgatc aacctggcgc atggcggcgg aggtgcagac tttgctgcca ttcgcgccgc
     1621 catggtcgat accgcggtgc aggtagcaga ggtggcccat gccgcgggtg cacgccgcct
     1681 ggtgcatgtg ggctccatcg ccggtcttta tctgggagac gcgggcgaga cggtgcgcga
     1741 cgacaccccg cccgacccgc tgccacaaac ccgcaatgac tacgcacatg ccaaggcgct
     1801 ggccgacgct gccgttctgg acgttcatcg gcggttgggc ttgcccgtgg tattgctacg
     1861 tccgggcctg gtggttgggg cagggacctc gcccttccat ggcggcctcg gattcttcaa
     1921 caacgaccaa tactgcgttg gttggaacga cgggcgcaat gccttgccct gggtgttggt
     1981 gacggattgc gccaacgcca tcgtgggtgc gatgacctct gaacaggccg tggggcgggc
     2041 ctacaacctg gtgggcgatg tgcgcccaac ggcccgcgag tatctggctg acgtagccca
     2101 ggtcacgggc agaccgttga agttcgtgcc ttcgtcgccg gttgcactgt ggctggcaga
     2161 aatgggcaaa tggctggtca agcgggctac cgggcggcgc gtgcagcgcc cgtacttgcg
     2221 ggacatccgt tcgcgtggca tttccgccat gttcgattgc agtgctgcca agcgggatct
     2281 ggattgggcc cccgttgccg atgccaagac gttccggcag atggccgtga tggtgcatgc
     2341 cgaggagccc gtcgcgtgac aaccgaacag cggccccggc atttgctgca tgccttctcc
     2401 acctttcagc tgggtgggcc gcaggcgcgc tttgtcgaac tggccaatgc gctgggcccc
     2461 atgttccgcc ataccgtggt tgccatggac aaccggttcg atgccggcga gcgcctgcac
     2521 ccttcggtca attgggcgcc catgaccctg aaggtcatca agggcggtgc gctggccaat
     2581 cgagcttcgt tccgccaggt gctgcgcagc ctgcagcccg atttgttgat gagctacaac
     2641 tggggtgcca tcgaatgggc agcggccaac ctcccccagg tttgccacca ggtacatgtg
     2701 gaggacggtt ttggccctga agaggccgca cgtcagctgc ccaggcgggt atggatgcgc
     2761 cgtgcattgc ttggctgggc aagggttccc gtggtggtca tctcgcgcca gctggagcgc
     2821 attgccctgc aggaatggag gttgcctgag caccgtgttc acttcattgc caacggcgtg
     2881 aatgtgccgg cacgcatgcg cccgcagcgt gagttggtgg ccggcgcagc cattcacgta
     2941 ggcaccgtcg ccggcttgcg gtcggaaaaa aatatcgctc gtctgatcaa ggcattcgcc
     3001 cgcctgcgtt cggtacaacc ggcgcgcctg accatcgtgg gtggaggact attgcaagct
     3061 gaattacagc aactggccca gtcgcttggt gtgggtgatg acgttgagtt cactggttac
     3121 ctgagcaatc cgggcgagaa gctcaaggac tttgatctat ttgccctgtc gtccgatacc
     3181 gagcaacagc ccattgcact gctggaggcc atggcaatgg gcatgcccgt cgtagctact
     3241 gcggtgggcg atgtgccggc catccttgcc gacgtgtcac cggacaatgt ctgcccgcca
     3301 gacgatgcgg cttttgcgca gttgctgctg caggttgcgc aggcaaacag caaatggtcc
     3361 gcttggtctt ccagagggca tgccttggtc agcagcacct acgccaagcc agccatgctg
     3421 gcgcaatggc aggcagtatg ggccggacaa ttggcgggtg tcagggcgac cagtttgagc
     3481 cacgcttgat ttatgcgaat ccttcacgtt ctcgaccatt ccattcccct gcacagcggc
     3541 tacacgtttc gcactgccgc cctgctgcgc gagcagcggg cgctgggctg gcaaaccttt
     3601 cacctcacgt cccccaagca aggcgttacc gaacaggcct tggagaccgt tgacgacctc
     3661 agcttctatc gcactgccgc tgcaacgggc gcggcatccg gcctgccgtt gcttggtcag
     3721 tggcagctca tggggcagct cacgcgcagg ctgcgcgagg tggcccagga ggtgcggcca
     3781 gacatcattc acgcccactc gccggtgctc aacgccgtgc ccgccattcg cgtggggcgc
     3841 gaactggggc tgcccgtggt gtacgagatt cgcgccttct gggaagacgc ggccgtcgat
     3901 catggcacca cccgtgaagg cagcgcgcgc taccgcctca cgcgtgccct ggagacctgg
     3961 gccatccgca aggccgacca tgtattcacg atctgcgagg gcctgcgctc cgatattgct
     4021 ggtcgtggcg tcgatcccgc caaggtgact gtcattccca acgctgtcga tacccaggcc
     4081 ttccaggcat cgcaggcgcc cgaccccgcc ttgaagcggt ctttgaacct cgatggcaag
     4141 ctggttctgg gctttgtcgg ttcgttctac gcctacgaag ggttggactt gctgatccag
     4201 gccttgccgg cgatcctgcg ggccaatccc gccgtggcct tgctgctggt tggcggtggg
     4261 cctgaggacg cgcgcctgcg cgagcaggta tctcgcctgc aactcgacgc ccatgtggtg
     4321 tttaccgggc gtgtgccgca caagcaggtc agccgctact acgacctgat cgacgtgctg
     4381 gcctatccgc gccattccat gcggttgacc gaactggtca cgccgctcaa gccgctggaa
     4441 gccatggcgc agcaaaagct ctttgtcgca tccgcagtcg gggggcataa agagctggtg
     4501 gagcacatga agaccggcgt gctttttgaa gccggcaaca aggatgcgtt ggcacaggct
     4561 ctgctggacc tgctgaacca caggcaggcc tggcccgctc tcaaggccaa cggccgggca
     4621 tttgtcgagt cggtacgcaa ctggcgcaac agcgtcgcca actacaaacc cgtgtatgaa
     4681 cggatcacgc agaagaaggt gcaagcatga aggtgatggt ggtgtttgga acccggcccg
     4741 aggccatcaa gatggccccg ctggtcaagg ccctgcagca ggcagcgcct gcgttacaga
     4801 cagtggtgtg cgtcaccgcg cagcaccgcg aaatgctcga ccaggtgctg cgcctgttcg
     4861 acctgcagcc cgagtacgac ctgaacgtga tgaagccagg gcaggatctg ttcgacatca
     4921 ccacccagat cctcacgggg ctaaaacccg tcctggcggc cgagcagccc gatctggtgc
     4981 tggtgcatgg cgatacgtcc accaccctgg cggccagcct ggccgcgtac tacacgcggg
     5041 ccaaggtggg gcatgtagag gccggcctgc gcaccaataa caagtgggcc ccgttccccg
     5101 aggaaatgaa ccgccgcctg actggcgcca tgaccgacgt ccacttcgcc cccacggcaa
     5161 cggccaaggc caacctgctg cgcgaaggcg tctctgccga cgccatccac gtcaccggca
     5221 acaccgtgat tgatgctctc ttggccgtgg tggacaagct gcgtgcagac gcggagctgc
     5281 gccagcgcct ggacaaggag ttctcgtttc tcgaccccaa ccgccgcctc atcctggtta
     5341 cgggccatcg ccgggagaat tttggcgaag gcttccagaa catctgccac gcattggcag
     5401 acattgcctc cgcccaccca gacgtgcaag tgctgtaccc ggtacacctc aaccccaatg
     5461 tgcgccagcc cgtgcacgac atcctggcgg cgcgtcagct tgataacgtc cacctcatcg
     5521 accccgtgga ctatctgccg tttgtttacc tgatggacag ggcgcatctc atcatcaccg
     5581 actcgggcgg tgtgcaggaa gaggcgccct cgctgggcaa gcccgtgctc gtgatgcgcg
     5641 aaaccaccga acggcccgag gctgttgagg ccggcacggt gcgcctggta ggcacgaatc
     5701 ggcaaaagct cgtggccgag gcagagcgcc tgctggccga cccagctgcc tatgcggcca
     5761 tggcacaggc acacaacccc tatggagacg gccaggcctc gcaacgcatt gtcaaaacac
     5821 tattaaattt gaagaacaag gagtaatgcc atggcgcgcg aactgcaaaa aattatcgta
     5881 atgggcctgg gctacattgg tctgccaacg gcctccatgc tggccaccaa gggccaccag
     5941 gtgcttggcg tcgatgtcaa cgcacaggcc gttgacatca tcaattcggg ccgcatccac
     6001 atcgtcgaac ccgacctgga catcctcgtc aagtcggcag tcaacagcgg caacctgaag
     6061 gcgtccctga cgcccgagga gggcgacacg ttcatcctgg cggtgcccac gccattcaaa
     6121 gaggtggacg gcaaccccaa ggcgcccgac ctggcctata tcgagaccgc cacgcgtgcc
     6181 attgcaccgt atttgcgcga agacaatctg gtcattctgg aatccacctc gcccgtgggc
     6241 accaccgagc gcgtgcaaga aatcattgtt gccatgcgcc ctgaactggc tggcaagatc
     6301 cacacggcgc attgccccga gcgtgtcttg cctgggcaaa tcctgcgcga gctggtggat
     6361 aacgaccgca tcatcggcgg caccaccaag gccgcagtgg caaaggccaa ggcgctctac
     6421 aagacctttt gcaacggcgc cattctggag accgacagcc gcacggcaga aatgtccaag
     6481 ctggtcgaaa actccttccg tgacgtgaac atcgcgtttg cgaacgagtt gtccgtcatc
     6541 tgcgaccacc tgggcatcaa cgtttgggag gccattgccc tggccaaccg ccacccgcgc
     6601 gtgaacatcc tgcagcccgg gccgggcgtg ggtgggcact gcatcgcggt agacccctgg
     6661 ttcatcgtgg ccagtgcacc cgcccagtca cgcttgattc gtaccgcgcg cgaggtcaac
     6721 gacgccaagc ccgactgggt gatcgccaag gtcaaggcca aggccgcacg ctttcacgag
     6781 cccgtcatcg gctgcctggg cctgagcttc aaggcgaata tcgacgactt gcgagagtcg
     6841 ccatccatgg acatagtcca ccacctgcac cagtcgggca taggcaagat catggcctgc
     6901 gaccccaatg tgcgcaaaga gaaggcgccc tttgcgctgt acgacctgaa aaccgtgatc
     6961 aaggaggccg acatcctctt gctgctggtg gatcacgaag agttcaagga catcgacccc
     7021 caggtcatca aggacaaggt ggtcatcgat accaagggcg tgctgcggtg atctgctctg
     7081 ccttgcttaa cgcgttattc gcccggggtg gggcgtgaag attcttctct tctccaccct
     7141 gtaccccagc gcggtgcggc ccatccacgg catcttcgtg gagacgcgcc tgcgcgagct
     7201 gctcaagact ggcgaggtgc aggctaaggt ggtggcgccg gtgccttggt tccccttcaa
     7261 ggcggcgcgg tttggctctt atgccaagtt tgccgccacg ccgcgcatgg agcagcgcaa
     7321 cggggtcgaa gtacaccacc cgcgttacct gctgccgccc aaggtgggca tgaacatcgc
     7381 cccatatgcc ctggccctgg gtgcgctgcc cacgctacgc cggctgcaac gcaacgggtt
     7441 cgactttgat ctgatcgacg cccactatta ctaccccgat ggcgtggccg ctgcgttgct
     7501 ggccaaatgg ctgggcaagc cgttcgtggt gacggcacgt ggttcagact tgaacctcat
     7561 ctcgcagtac ccgcgtccgc gcaagctgat cctgcaggcg gccgaacagg ccgcatcttc
     7621 catcggcgtg tgccaggcgc tcatggacaa gctggcggag cttggggcca atccgtcaaa
     7681 gctcaatgtt ctgcgcaatg gcgttgatct ggagcgtttt gtccccgaag accgacaagc
     7741 ggcgcgtgag cgcctgggcc tgcccaaaga ggggagctac ctgctgatgg tggcgaacct
     7801 ggtggaactc aagggccacc acatcgccat ccaggcattg gcacacttac ccggcgtaac
     7861 cttgctgatt gcaggcgctg gcccggagca ggcggccttg aaggccctgg ccgcccgcac
     7921 cggcgttgcc gaccgggtgc attgggcggg cctggtgccg caagacgagc tcaagtggtg
     7981 gtacagcgca gccaacgctt tggccctgtg cagcagccgc gagggctggc ccaacgtgct
     8041 gctggaggcc atggcctgcg gcacgccggt gctatccacc aatgtctcgg gtacacccga
     8101 catggtgaac tcccccgtcg caggagagct gctggagagc cgaagcgcac aggcgttggt
     8161 caaggcctgg acacaattgc aagcgcgctc acctgcccga gccgcaacgc gcgcccatgc
     8221 cgagactttt tcctggatgg cgacgacgca ggggcagctg cagttgtttg gaagggtgcg
     8281 acatgcacag gcatgcgttg atcatcggtg agtttgagaa tgtggcacgc gttgcgacgc
     8341 aagccgagtt ccctgcgtac gaggagcgat cagtaagcta ccgcaacaat gggcaggtcg
     8401 cgcaatagcg cacagatcga tatattgaac tcaaaataat caactgtcgt attaccgccg
     8461 gttgaatttt tcaaatggag attttctgtg gaagcattca ttctggtgat tgatctgggg
     8521 gtgatgctgt atttatgttg gtgcatattt gtagggcgtg atgaccacga tatcgatctc
     8581 ggatatctac gctatgacaa gaccaatcag tcgcactgag ccttcgtgtg ttctgaagtg
     8641 cacgaaagca tatgcgtgat ctgatatttg ctttgatgct gctcgtggcg ttgcccctgg
     8701 gcatggctcg tccattcaac gcctacctgt tgtgggggtg gactgccatg ctggcaccca
     8761 ccacctattt ttatggcttc atggtggggg cgcgcgtgaa tctggtgtgc gccctgctga
     8821 ccttgttgtt gctggcgttg gggcgtgtgc cgtggcgcaa ttatcaaaac aatgcggtga
     8881 cctggcttta tgtgctattt gccatccacg caacgctggc gtactggttt gcgtatggcg
     8941 acaaccccaa caacgatcag tactatgagc tcctacttaa aggactgttg ttcgcggtgg
     9001 tcatgccgtt tttcgtgcgt gagcgcgtgc attttcacgc catgttgatc gtcattgccc
     9061 ttgggctggg ggttcacggt gtgctgaatg ggctcaagac agtcgtatcg gccggcggac
     9121 atctcatgct gggcccgcaa ggtaccatgc tgaacgatcg aaaccatctt tctgtggcgt
     9181 tggcgctggt actgccgctg ctgttttact tgcatagcta cacggtcaat cgactcatcc
     9241 gcctcggcta cttgggagcc ttttgcgtgc tggtgctggc cattcttggc ggtggctcgc
     9301 gtggtggttt catcgcggtg tccgttgtgg gggcttggtg gattctcacc acccgtcgca
     9361 agggtttggc ggtggttctg gtaatggctg ccgttatggg gtttcttgcc tttgcgccag
     9421 aaaatatcac caatcgaatg tccactattg aggatatgaa tgatgatcaa tccttcatgg
     9481 gacgggtgtt tgcctggagg gtgagttctg ccattgccct ggccaatccg gtctttggtg
     9541 gcgggtttca cgcggtgcag gttcagtgga tctgggacag cttcaaggca gacccggggc
     9601 ttcttggttt tctgcaattg cctgttccga cattctcggc caaggcggca cacagcatct
     9661 acttcgaggt tatgggtgat ctgggttttg tagggttggg gctgttcctg ctgatactgc
     9721 tgcgtgcact gtggagtcga caggccatca aacgcgtaac cacccggctt ggcgcgccct
     9781 accagtgggc tggcgacatg gctgacatgt tgatgttggc ggtgctggcc ttcatggtgg
     9841 ggggggcggc ggtcagcctt ggctattacg aggtaattta tatggtggtg atgctcatgg
     9901 agttgttgcg gctccatgtt gggcgggcgc gggccgatac ggtaggccag gccctgcccc
     9961 gtaagggggc gatgtgattt cccgcctggt tctgcagtcg atgtccaagg gcaagttgtc
    10021 ggtattcctg ttccataagg taccccgtca gcgcgatccg ctgatcccgg cggaggttga
    10081 tatcgccaga ttcgagcacc tcctggatac tacgttcacc aagctgcaag tgctgccgtt
    10141 gggcgatgcc ctggcgctgt tgcaggcggg ccgcctgcca cggcgtgcgg catgcatcac
    10201 cttcgatgat ggttacccgg attggctgtc gggcgtcgcg ccattgcttc tgcgccgcaa
    10261 cctgcatgca actttcttta tcacctcggg gcagtttgat ggcgtgccac tgtggcacga
    10321 gcgcatcttg gctgcagttc gccgtctgcc cggttccgtg ctggacatgg gaatcccctt
    10381 tctgccagcg cggcctgtag cgaccatggc cgaccgccag cgccaggtgt tgcgcctgga
    10441 acaagagttg aaatacctca cgcttttccg gcgcgagcag atcctgcagc agcttgaatc
    10501 catggcaggc gtgcagcctg cccaagtgcc ggtgatgagc gaggctcagc tgcgcgactt
    10561 gcacagccag ggttttggca ttggtgcgca tacagcgctg catcccatcc tggactactg
    10621 caacgccgag gaagtcgaac acgagattgg tggtgcccgc gagcgcctgc gcgccatcgt
    10681 gggcggggcg gtggacggct ttgcctaccc caatggtcgc ccctatgctg atttttcgcg
    10741 cttgcacgta gacgcagtca agcgtgccgg ctaccgctat gcggtcacca cgcattgggg
    10801 tgtggccgat accagtacct cacccttcca gattccgcgc ttcactccgt gggctgagcg
    10861 tgattggcat atgacctttc aattggcgcg caatctgata accaagccgt tgcaagttcc
    10921 ggagtcgact gcatgactgt gacgactacc gtgaaagatg tattgcgcac agcgctttac
    10981 cgttgtggtc ttttgggcgg tctgcaccgc tggcgtaacc ggcacatact tacagtgctc
    11041 atgtttcacc gggtcttgcc ggccgatgat cctgctttgg cgcaggcgga ccgcgagttc
    11101 accttcacac gggaggggtt cgggcgtacc ctggactttg tgcaacgcca ttacaacgtg
    11161 atcagcctgg atacgctgca ggccgcccgg caggggcgtt ctaagttgcc accaacccca
    11221 ctgttgatca cctttgacga tggctggcgt gacaccttga cccacgcact tcctgagctt
    11281 gagcgccgca gcttgcccgc ggtgttgttt ttggcaagtg aagtactggc gctgaaagaa
    11341 ccgcgttggt ggcaggatgc gctgatcagt gcgctccaac agcccggcgc cttggtacgt
    11401 ctttctcagg ccgcgggctg gacggatagg gtacaaggcg acgtaagcca ggcgctgaca
    11461 gcccatcttg cggctatgcc ggaactccag cgctggatct ggctgcaaac acatgcgccg
    11521 gatgttgcga cgcatgtcat ggatcgccag atggttaccc tggacgaatt acaggctatt
    11581 aacggcagct ctctggcgat gggggggcat ggccacaccc acgggccatt gacacgatca
    11641 acgaacccac aagccgaact gcaggccagt catgcagtat tggctgttct gggccaacgc
    11701 gtgcatagca tgtcgtttcc ccacggcgcc tggacacatg aactcgtagt acaggcccgg
    11761 atggtggggt ttgagtgggt attcaccagc gagcccacgc tggccgacgc gtcgtgctta
    11821 tcagattcac ttccggccct agggcgcatc cacgttccag aaaacacctg gacctgtaaa
    11881 aacggctgca ttgcgccgga acgcctggct acgttcctgt ttttccgcac tgtgcagcaa
    11941 tagtgtgcgt acacattttt gggcgcaggc tatctgggaa gggcagtgcg tacggttgat
    12001 ttccgcatcc ccgtccggag ggggaggggg cctgcgacgc tcgcactctc atgtcggtaa
    12061 gctaccgaag aatcctccaa gctccatcac aatcttcctc caggcaagaa atggccaaaa
    12121 ataattgctg aataagcgcc ttacgctatc aataaatgtg catatacaag tccttaaggg
    12181 ggcgagggag tcccctcagg cagcatgtgc tgcgcgctac caaaaggatg agtggtgccc
    12241 ctatcccgaa ggggaaacgt ttgcgacagc ttgccaaccc agtgataccc ctagttatga
    12301 gtgctgaccg cttgcgcatc caacgcaaga ctttttgtgc gctgtcccac gcagagctgg
    12361 ctgcatggtc acggctgctg cgcgacagca ccagcacgcg ctgggcgttt ctgtctccag
    12421 tctatgccgc agccgtgcat gagtgcattg gtacggtaga agtgttgctg tgttggcgtg
    12481 gtgacgagtt ggccggtgtc atgctgctgc agcgtgcgcg gggctggctt gggcgcatgg
    12541 ggcttaggga gccggcaggg gggcagatgt cggactattt cggtttgctg gcaagcccag
    12601 gggttcagtt gcaatggtca gtattgttga atgcggcggg tcttcactgt ctttatttca
    12661 cccatctcga cgaaacccag gcggcgcatg gcctcgtcgg acaggagtgc agaatagggc
    12721 tgcgcacccg catccacccc gatggcggtg ctgcccattg ggaatatttg cgcacgcaag
    12781 acaaaaagct ggtcagtgac accgagcggc gtgagcgcaa gctggcggcc gagcatggcg
    12841 cgctcgtgtt cgagatgcaa acgcccacgc cgcagcagga tctggattgg ttggttaccc
    12901 tgaaaaatgc gcagtaccag cgtaccgggc atgacggtgg ggccttgctc gacccagcca
    12961 acgcacgttt gctgcagcgt ttgttgacgt catcggatgc ctactgccag cccagactca
    13021 gcgtactgcg gtcaggtggg cagatggtgg cggcgcattt tggcttgcaa tgtggggcgg
    13081 tgctgcacta ctggtttccg gtctacgatc aacggtttgc tgtcttctca ccggggcgca
    13141 tcctgtttcg ccatattttg gctgtatcgg atttgcatgg cattgcttgc attgaccggg
    13201 gcgagggcga cagcgcggcc aagcgggact tcgccaacga agagcacgtg ttttgcaaag
    13261 gcattgtgac ggcgggcctg tggggccaag cattgcgcgg tgttcagagt gcctgctggc
    13321 gcctcacggc ttgattatgt ggcgggtggt tgccctggag ccttggcgcg ccaatacgac
    13381 aggcagatat agaccaggct acgcaggggg cggtgcccat tgcgcagcgc ctgccagaag
    13441 gcagcgcggg cgatggtgcg atctccgcgc caataatgcg catagccatg gtcgaagtga
    13501 acgcgggtca ggcgtttctg aaaggctcca gaccccaggc tttggccgcc gagcgtcttc
    13561 agcccccagc gcatggacgc tgcctttagc aggttgacca aatggctttc ggccgaaagc
    13621 cggtgcatgg cgctggcgcc gtggatgcgg tacagggcca ccgggccatt gagcgaatgc
    13681 atttctgcga cccgtgacgc cttgagccag aagtcatagt cctcgccctg gcgcatggct
    13741 tcgttgaagc cgccaatcgc ttcgtaaaca ccacggcgta ccacggcagt gttcatgcct
    13801 accagcaaac catcaaggag gcgggtgtag agccagcccg accgctcagg atccatgcgt
    13861 gtgaactggc ttgcgtcctg cacaagcgag gccgcagggg caaaactgcc attggccaag
    13921 gcaggccagc ggatgaactg gccgaagacg acgccaacat cggggtgctg gtcaaagtac
    13981 gtcacctgcg tttgtagctt gcctgggaac cagatgtcgt cggcatccag aaaggcgatg
    14041 aattcaccac gcgcctgcgc catgcctaca ttgcgggcgc acgagacgcc caaaccactg
    14101 aggtggagca cgcgaacgcg cgcgtcctgc agcgcgtagc ggtcgtagtc gtcgtccgtg
    14161 gagccgtcgt tgaccagcag cagctccagc tcggtaaacg attgttgaag cacactgacc
    14221 agggcttcct gcaaataatc tttgccgttg cgcaccggca tgatgacgga gacgcggggt
    14281 gtctcgcggg ggggcggcgg gctttgtttc atgggcattg tctccgttgt gcgagggcct
    14341 tgcgcatgcg tcgcaattca cctgccatgg gcagcgacag cgcaaagcag cctaccaaga
    14401 aggccagtgc cgcacaggca ccggtgacta ggatggcaac ccacggggca atgtcttgcg
    14461 gcaccgcctg ccgcgtggct aggcagatgc cgcccaccag caggccctga gccaccaagg
    14521 gcacggtgct gcggccccag gcagtccagg tcagattcag atgacgctta tggatccaca
    14581 ggtagaaggg tatgccgagc aattggccta gcgccatggc caacgcgaag ttgtgcaggc
    14641 tgccgttcat gagccaaagc gcaaacaggg ccttggccag caatacgact gtgttggggc
    14701 cgattgccgc ataaggcttg ccaacgccag tcagggcagg ggtactcaat gtgaacaggc
    14761 tgctgacggc gtaggacagg cacagccagg ggatggccgg agctgctgcc atccaagcct
    14821 ggccgtacag caggctaacg atgtcatgcg ccagtaccgc aatcgcggcg aaggcgggca
    14881 gcatcattgc attgataagg gagctggcgc gcagatattc gccgcccact ggcccctggg
    14941 cgtgatgcac cttggccaaa tgcggtagtg cgaaataatt cacggttggc agcagcgccg
    15001 cattcgccat gttcacggtg gaattggccc ggctgaacag gcctactgcg gcgggcgtca
    15061 tccagcgacc cagcatgacg tcgggcaatg cgttgtctgc cgccttgagc agggccgtga
    15121 acagattgcc caggccgaaa ttgacgatgt cgcgcacttg gccaagcccg ggcagccacg
    15181 gcagtgggcg ccccaccatc cagcgcgcca tggcgccggc gacgatgata ttgaccaggt
    15241 tggcccaggc catggtcatg gcgccaaagc cattcaacgc caggaggatc gaagtgatga
    15301 aatagacgac ggtgcttgct gccgtcacgt aggcagtttt ttcgacggcc aactcgcgtg
    15361 tcaggatggc catgggaatg gcgccaaacg gtatgaagat gaagcccagc gccagcacct
    15421 gcaccaccgg taccacctga tcttcgtgaa aaaaatgagc ccagtaccgc gcactcaaaa
    15481 acatcaacgc tgccaccgtc caagaggtcg caagcaacac gccgagggca ttgcgcagca
    15541 tctcgggcct gagctccttc tcgcgcttga tgaaggctgt tacgccaaag tcgcgaaata
    15601 catgggcgac ggcgatgagc accgcgctca tggaaaaaat gccgatttcc tgcggcgtaa
    15661 gcaagcgtgc caacacgatc gacagcgcaa agttggccgc cgtggccagg ttgctgaaga
    15721 agaactgcag ggcgactttt tgacgaacgg agggcacgtg agataggtgg gcgggcgtcg
    15781 cctgtgcagc tggatgatgt atgccgacgg caggggctgg gcgtggttgt ggcaggcggt
    15841 gaaggacagg atcaaattgg gatgctactg gttctatgat tcaccgaacg ctatcttcct
    15901 cgatttgttg cccatgaatt tttcagactc aaccattgcc atcatcggcc tcggctatgt
    15961 tggcctaccc ttggccgtag agtttggcaa gcaccgcccg gttttgggct tcgacatcaa
    16021 ccaggcgcgt gtcgaggagc tgcaggccgg acgcgaccac accctggaat gcagtgccga
    16081 ggagctacgg gcggcccgtc acctgcgcta cagccaccag ctgcaggatc tgaaggcatg
    16141 ccaggttttt atcgtcaccg tacccactcc ggtggaccag gccaaccggc ccgacctgac
    16201 acccctcatt aaggccagtc agaccgtagg tgcggccttg aagcggggtg acgtggtggt
    16261 ctatgaatcc acggtctttc ctggagccac cgaggaagtt tgtgtgcccg agctcgagcg
    16321 cgccagcggc ctcaagttca accaggattt tttttgtggc tacagtcccg agcgcatcaa
    16381 ccccggcgac aaagaacacc gccttcccag catcaagaaa gtcaccagcg gcagcactcc
    16441 cgaggtggcc gacgccgtca acgcgctgta tgcccagatc atcaccgctg gtacccacaa
    16501 agccagctcg atcaaggtgg ccgaggccgc caaggtgatc gagaacaccc agcgtgacat
    16561 caatattgcc ctgatgaacg agctaagcct gatctttgca cgcttaggca tcgataccct
    16621 cgaagtgcta caggcagccg gcaccaagtg gaacttcctg cctttccgcc ctggcctggt
    16681 aggcggacat tgcattgggg tggatccgta ttacctcacg cacaaggcgc aggaggtcgg
    16741 ttatcaccct gacgtcatcc tggcaggccg ccgcgtcaac gacaacatgg ccgcccatgt
    16801 ggccgacgcc accatcaagc tcatgctgcg caaaggcatt ccggtgctcg gcagcaaggt
    16861 attggtgctg ggcctgacct tcaaagagaa ctgccccgac gtgcgcaaca ctaaggtggt
    16921 ggacattgtg cgcaccctgc agcgctataa cgctcaggtt gatgtattcg acccctggat
    16981 cgacgcgcgg gaagccgagc acgaatatgg gctgcagtgc ctgaaagcca taccggggcc
    17041 tgggcaatac gcggccatca ttctggccgt tgggcaccgc cagttcgtgg agttgggcga
    17101 atccggcatt aaggcgctgg gcgaatccgg cgctgtactt tttgacgtaa aaagcatcct
    17161 gcccctgggt gccgccgacg ggaggctgtg agcatgagta cccgctatca ggccgtgcaa
    17221 acgcagttgc aggcagagtc gaaaacctgg cttatcaccg gggtggccgg cttcatcggc
    17281 agcaacctgc tggaaaccct gctcaagcta gatcagcgcg ttgtcggcct ggacaacttt
    17341 gccaccggcc accggcgcaa cctcgacgag gtgcaaaccc tcgtcagccc cgcgcaatgg
    17401 gccaacttcc aattcatcga aggtgatatc cgcatcctgg aagactgccg tcgagccctg
    17461 gaattcctcc cttcccctat gggggagggc aggggtgggg gccagcccaa tccgattcct
    17521 gtcgattacg tcctgcacca ggccgccctg ggcagcgtac cccgcagcat cgaagatccc
    17581 attaccacca acaacgccaa catcaccggt tttctaaaca tgctggtggc cgcacgcgac
    17641 gcccaggtgc aaagcttcac ctatgccgcc agcagcagta cctatggcga ccaccctggc
    17701 ctacccaaag tggaggacgt gatcggtaaa ccgctatccc cctacgccgt gaccaagtac
    17761 gtcaacgaac tgtatgccga cgtgtttgcg cgcacttacg gatttcagac cataggactg
    17821 cgctatttca atgtcttcgg cccgcgccag gatcccgatg gtgcctatgc agccgtgatt
    17881 ccaaaatgga cggctgcaat gattcaggga caggatgttt tcatcaacgg cgatggcgaa
    17941 accagtcgcg atttttgctt tgtcgacaat gctgtgcagg ccaatctgct ggcagccacg
    18001 gtaaaagatt gtagcgcacg aaaccaagtc tataacgtgg cgatttctgg ccggactacg
    18061 ctcaatgggt tgtttgaaga attgaaattc gtgctggcaa ccaatggaac gtattacaca
    18121 aaaaaaccaa tatatagaga ttttcgttct ggtgatgtgc gccatagtca agcggatatt
    18181 ggcaaggccc aatatctcct gggctatgta cccagtcatt gcattcaagg aggaattata
    18241 aaggcaatgc catggtatat tcggttcttt atgtaaaatt tgacaaggca ctactcaata
    18301 gctacccata tgggactttc tttgatgtta tatctagaat caaaatcagc atggtgcaag
    18361 gacattgcca tcgtgctggg tgtagtggcg ctggtatccg tcgcagagat ttttctggtt
    18421 aatagaaaat acggtatttt ttctggaggg tttggccagt ctcgagtgct ggacagtgca
    18481 tcggatgtac tggtatttgc cgttgcttat ggcattgctc aattattttt ggccatgatt
    18541 gtgtggcgca tctcggttag cctgacaaaa aagcaaccca attgggctgc cgccttcttg
    18601 cttcttgtct tgatgggtgg aggatttgtg ctggccctct ccgtgcaata taagctccac
    18661 agttatttta gtgatgcggt gagcttcgcg ctaatcagtc ggccctgcaa aacccagcca
    18721 gcccccataa acactgggtt tgcgctgttt ccccgacagc tccatcccc
//
LOCUS       DAOMTQ010000049        18753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_391_length_18753_cov_26.440314, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000049 DAOMTQ010000000
VERSION     DAOMTQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 18753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18753
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_391_length_18753_cov_26.440314"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..2109
                     /locus_tag="PK518_06765"
     CDS             223..2109
                     /locus_tag="PK518_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM48653.1"
                     /translation="MALITLMEAQLAFGHVALLDHASFALEPAERVGLIGRNGAGKSS
                     LLKILGGLAKADDGSLSVQQGLRITYVAQEPELDPEATVFAAAAQGIAQAIALREQYL
                     SGAPGLDLDALQSQIEAYDAWNWEQRVEETLQRLHLDPQALIGTLSGGTKKRVALAQA
                     LVARPDVLLLDEPTNHLDLDSIEWLEELLLAFKGSVVTITHDRAFLDRIATRIVELDR
                     GQLRSYPGNFAQYQAQKEEQLAQEAVINARADKLLAQEEVWIRKGVEARRTRSQSRIG
                     RLEALRANRAARREALGSVRMDIASGGQGGYQGKIVAELTRVSKAFGDRAIVRDFSAT
                     ILRGDKVGLIGPNGAGKTTLLKLILGELTPDSGDVRRGNNLQVAYFDQMRNAIDLDAT
                     LEDFISPGSEWIEIGNQRKHVKSYLGDFLFSPARAHSPVRTLSGGERNRLLLARLFAR
                     PANVLVLDEPTNDLDIDTLELLEELLQSYDGTVFLVSHDRSFLDNVVTSTIAAEGDGL
                     WREYEGGVQDWLTQSRRSRELAGSAAPAAKNTSQNEAKRITDKREQLQKKKLSYKEQR
                     ELEQLPAQIDALEAEQTAIRDELADGSLYARDGTRAAALHARDGEIEELLMQALERWS
                     ELST"
     gene            complement(2116..3225)
                     /locus_tag="PK518_06770"
     CDS             complement(2116..3225)
                     /locus_tag="PK518_06770"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRM48654.1"
                     /translation="MKPKIRVIVVDDSALVRSLLAEIINRQGDMECIGTANDPLIARE
                     MIREQNPDVITLDVEMPRMDGIDFLGRLMRLRPMPVVMISTLTERGAEVTMKALELGA
                     VDFVAKPRVGLSSGLNELATQIVEKIRVAAAAHVRRMPVRAATTAAHPDGTAQAAPAP
                     ASAATLLGRVSTEKVICIGASTGGTEAIREVLVHLPADSPAIVITQHMPPGFTTSFAA
                     RLNSLCQITVKEAVNGERILPGHAYIAPGGKQFHIARSGANYVAVVDDGPPVNRHKPS
                     VEVLFKSAATVLGRNAYAIMLTGMGADGAAAMREMKDAGSYNYVQDEASCIVFGMPRE
                     AIAHGAADEVLPLTQIAPALIARLRGATDRLHHRI"
     gene            complement(3244..3963)
                     /gene="cheD"
                     /locus_tag="PK518_06775"
     CDS             complement(3244..3963)
                     /gene="cheD"
                     /locus_tag="PK518_06775"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HRM48655.1"
                     /translation="MNPQSMAPPPQPGALTERRRIPRVTALEGEAQAQPAQTLQQLKA
                     QARKPGEASFFFFDHHFQHNAAKVLPGEYFVAREPLAIMTVLGSCIAACLWDSRMRVG
                     GMNHFMLPDGDNGDSSGRYGSYAMELLINEMMKLGARRETLQAKIFGGGQVMANFTTM
                     NVGERNTRFVQDYLATERIPVVSEDVLDIYPRKVVFFPVTGKAMVKRLAHAHPEATAQ
                     EARGNAASVARATSGGSVDLF"
     gene            complement(3975..4886)
                     /locus_tag="PK518_06780"
     CDS             complement(3975..4886)
                     /locus_tag="PK518_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRM48656.1"
                     /translation="MHAHRQAIATPTARRAHVRVGQHHTAANAEHEDASGREFAWTQT
                     DFSRVQALIYQRAGINLHDGKHAMVYSRLSRRLRETGHTSFHDYLGWLEGSDGPEWQE
                     FINALTTNLTAFFREQHHFEVLTQLLRTRPNGPWNIWCSAASTGEEPYSIVMTALEAL
                     APRAPFKLAASDIDSNVLAKAARGVYRQDGLKGLSQSQLQRFFLRGKGDNAGMARVRP
                     ELRQPIDFLSVNLIRDDWPFREPFDVIFCRNVMIYFDEPTQRRVLERMHRVLKPGGML
                     FVGHAENFSDSRDLFALRGKTVYERVG"
     gene            complement(4886..5374)
                     /locus_tag="PK518_06785"
     CDS             complement(4886..5374)
                     /locus_tag="PK518_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRM48657.1"
                     /translation="MNVVDKGIEIVAAGAREYLTFRLGQEEYGIDILKVQEIRGYEAP
                     TRIAHAPAFIKGVINLRGTIVPIVDMRLRFGCAQADYDSFTVVVILNLRERVVGIVVD
                     SVSDVMELAPEQLRPAPDLESSIDAQCIRGLGSVGERMLILLDIERLMAGVDMGLATA
                     AS"
     gene            complement(5411..7540)
                     /locus_tag="PK518_06790"
     CDS             complement(5411..7540)
                     /locus_tag="PK518_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRM48658.1"
                     /translation="MADTHQESAGADFDLTQFYQIFFEEAAENLDQMEQMLLNLDLEA
                     ANDEELNGIFRCAHSIKGGAATFGFADVAELTHQMESLLDRLRRHELRLIAPMVDVLL
                     ESADASRSLLARHQAGGQGEAISTHDLVRSISELASGQAPAMAPQAVVLAPQPAFAPA
                     PVAAPAVVPEVGADGMRALEIHIGPLEQLAQADAIKELFRDIPGLGQIEDLPGEVADM
                     RRFAVRTASSNDDLLDLFAFHVAKDKVSILAAGACPAPAPAAPVAQSPAPDAARPYGF
                     FAGAPGVPVDEVAVAALAAAAAPAQRTAAARAAPEAKGASQASMESSTIRVAVNKVDQ
                     LINLVGELVITQAMLAQNSRGLDAGAHQQLLAGLADLDRNTRDLQESVMSIRMIPMSI
                     VFNRFPRMLRDLAAKLGKKVDFITHGEATELDKSLVEKITDPLTHLVRNSLDHGIELP
                     EDRLAVGKPEHGSLTLSASHQGGSIVIEVRDDGKGMSREKILKKARERGLDVSDTMPD
                     SEVWQLIFAPGFSTADVVTDVSGRGVGMDVVKRNITSLGGSVEIDSAEGYGMRVSVRL
                     PLTLAIMDGMSVGVGDEVYILPLSSVVESFQVNEGDVNTVTQGSQLVKVREEYMPVMS
                     LERIFQVPRPGGAGSSGNNIMVVVEADGSRVAVLVDELLGQHQVVVKNLETNYRKVPN
                     VSGATILGDGTVALILDTGALVRRARH"
     gene            complement(7593..7955)
                     /locus_tag="PK518_06795"
     CDS             complement(7593..7955)
                     /locus_tag="PK518_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM48659.1"
                     /translation="MLSILAVDDSPSMRKMVSFTLTSAGYHVVEAVDGQDALEKAETH
                     PVSLVLADQNMPRLDGIGLTRRLRAHPRYKGTPILILTTESSDQMKQAGRAAGATGWL
                     VKPFDPVRLIEVIQKVIR"
     gene            complement(8023..8472)
                     /locus_tag="PK518_06800"
     CDS             complement(8023..8472)
                     /locus_tag="PK518_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48660.1"
                     /translation="MQSTLITPDEGPAVQVPVMVAADLQDCLLMAAHDLHRLEGLLDH
                     ATDNLLARFGEANAVLTDARVDGCAELLALRTALRSAVTELQFHDMATQLITHTSRVL
                     QASAFRLAAQVMGGEEGEDAAPTEGMAPDRPNPVAQREMAAGSVELF"
     gene            8583..9704
                     /locus_tag="PK518_06805"
     CDS             8583..9704
                     /locus_tag="PK518_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM48661.1"
                     /translation="MIPISTELEPLKSPMRILHLEDSTADHALVRLTLRRSGVAHEIR
                     QVETLDEFTQWVMQKNHDLILADYRLPGFTALDAWSVVEKQAGHPPFVLLSGAIGEAA
                     AVDAMRLGFADYLLKDDMARLPHVLQRALEVAEARRAREQAAAELAASEQRLADLAEH
                     LQTSIEEERAAIAREIHDDIGGALAAVKFDLAWLGRHSADDDQRRHAASATEMLQHAM
                     DASQRLMFNLRPPVLDQGLVAAVRWLAESFERRTRVPVRVSASREAMDMPPTLQLVAY
                     RTAQEALTNVHKHAQASRVHIDLSDHEGVLTLEVQDDGRGIASGMRQKPRSFGLRGLE
                     ERARRAGGWLDISSRPGQGTSVIMSLPLTSTEPDAGEES"
     gene            9701..10330
                     /locus_tag="PK518_06810"
     CDS             9701..10330
                     /locus_tag="PK518_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM48662.1"
                     /translation="MIHVVLCDDHAVLRRGIRDTLAESPEICVTGEAGSYAELREVLR
                     AATCDVLLLDLNLPGRSGLEALASLKESHPAIKVLIVSMYPEDQYALRCLKAGAHGYA
                     NKGGDPVQLIAAVKTVVEGRKYLTPEVAQLLADSLAQPTPELPHESLSERELQTLVKI
                     ASGKRLSDIAEELLLSPKTVSVYRARVLEKLKLANNAELTVYAIRNRLV"
     gene            complement(10429..11196)
                     /gene="fliR"
                     /locus_tag="PK518_06815"
     CDS             complement(10429..11196)
                     /gene="fliR"
                     /locus_tag="PK518_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRM48663.1"
                     /translation="MTFTEAQIMAWLSPVLWPFLRVLALFTAAPVFSMRAIPVRVRIG
                     LAFFVALCAQAVLPDQPVIDLNGREALGAVLQQVGVGLALGFAVRLVFVAVELAGEVI
                     GLQMGLNFASFFDPMANAQVSAVARFFGNIAMLLFVVINGHLMVLMAVVKSFDSFPVD
                     GHFLQAVGRMRLHELGASLFASSLWIALPMIAMLLFVNLTLGIISRVAPQMNIYAVGF
                     PVTLTVGMLGITATLPMLEQPMLTLLQQSMDLFGGGR"
     assembly_gap    11199..11298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11299..11525)
                     /gene="fliQ"
                     /locus_tag="PK518_06820"
     CDS             complement(<11299..11525)
                     /gene="fliQ"
                     /locus_tag="PK518_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRM48664.1"
                     /translation="MTSQFVLTMGRDALTLLLTISLPVLGVVMAVGLVVSIFQAVTQI
                     HEATLAFVPKLVAAVVVFTIAGPWMITTLVD"
     gene            complement(11698..12453)
                     /gene="fliP"
                     /locus_tag="PK518_06825"
     CDS             complement(11698..12453)
                     /gene="fliP"
                     /locus_tag="PK518_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914341.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRM48665.1"
                     /translation="MHKRLRAAALLGALLWAGVALAQQGGAAGTLPLVIGSGAGGNSY
                     SVPIQTLLFFTALSFLPAVLLMMTGFTRIVIVLSLLRQAIGTQQAPPNQVVVGLSLFL
                     TLFVMGPTLDKVYQEAYVPYTTNALSFEQALDKAEAPMRGFMLKQTRQSDFALFARLA
                     RLDDGVTAETAPLRVLVPAFVTSELKTAFQIGFMVFIPFLIIDMVVSSVLMSLGMMML
                     SPVLVALPFKLMLFVLADGWNLLIGSLAASFAV"
     gene            complement(12446..12784)
                     /locus_tag="PK518_06830"
     CDS             complement(12446..12784)
                     /locus_tag="PK518_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRM48666.1"
                     /translation="MTQTLLLLVLLVAALAALPWLIRRLQQQRAGQLGQAGAMSRVLS
                     AVAVGPQQRVVTVEVGPPQQRTVLVLGVTAQNINCLHVLPAPAADGTGFAAEMAAARA
                     QPLVDGGADA"
     gene            complement(12795..13244)
                     /gene="fliN"
                     /locus_tag="PK518_06835"
     CDS             complement(12795..13244)
                     /gene="fliN"
                     /locus_tag="PK518_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRM48667.1"
                     /translation="MSTESDHNDPNGAAAADDPFAGWAEALEEQKSADAQAVEADQGG
                     PLSGEPARPFGGNGNAQVQDINMVLDIPVQLSVELGRTKVPIKYILQLAQGSVVELDA
                     LAGEPMDVLVNGYLIAQGEVVVVNDKFGIRLTDVVTPSERLRRVSRG"
     gene            complement(13234..14238)
                     /gene="fliM"
                     /locus_tag="PK518_06840"
     CDS             complement(13234..14238)
                     /gene="fliM"
                     /locus_tag="PK518_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRM48668.1"
                     /translation="MSDSFLSQEEVDALLEGVTGESQKTVKEEIEAGAVRDYDISSQE
                     RIVRGRMPTMEIVNERFARNFRIGLFNFIRRSPEISVGAVSVQRYNAFLRELVVPTNF
                     NIVSIRPLRGSGLIVCEPSLLFGIIDTLYGGVGKFQTRIEGRDFSATEQRVINRLVEV
                     ICAEYKKAWQGIYPLELSYQRSEMQPQFANIATPSEIVVSTAFQLEIGDLSGAIHICM
                     PYATLEPIRDVLYSATQGDAIEVDRRWVRVLTREIQAAEVTLVAELARADATVEQLLA
                     MKPGDFIELERQPRIRATIEGVPVFDCQYGTHNAKYAIRVEECLRGIEAHWMGEKHVN
                     "
     gene            complement(14264..14845)
                     /locus_tag="PK518_06845"
     CDS             complement(14264..14845)
                     /locus_tag="PK518_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRM48669.1"
                     /translation="MSEPTAAPAKAKSKKLIIIVAALAVLLVAGGAAAWLLLGKRGGG
                     DEEEGHAPVAQAAAPKTPPTFLPMENMVVNLADPGGDRFAQVGITLELADAKTGELVK
                     SFMPSIRSNVLLLVSQRSTEELLTREGKEKLAVDIRREVSRPLGFTVPKPRKRPVQRD
                     PEDEEEDDEVAPRRAKADNNPVRQVLFSSFIIQ"
     gene            complement(15004..16401)
                     /locus_tag="PK518_06850"
     CDS             complement(15004..16401)
                     /locus_tag="PK518_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRM48670.1"
                     /translation="MEKTRIATPAPQSAHEARAARGTGQKPGSAEDAAIAAGQGGGFA
                     LLLAALGGGASEQASTQAGEQAGEQVGGAVALLDGLGTTAPEAGEQALKPQPGMPDGA
                     ALAPWMLGLQPTAVQAQGAGVVMDGAAATTGGEVAALSGLAGRSGQGLMGVAQAGTQR
                     WTSGSLVGETAMLDGAAEAAALGGTSQGMGAAAPAGRGVAARAQPWQPADGPSGAAGS
                     GRNNAAGVVSIAQTATESIVTMPAAAQTTGLAISERGGAQQPMPLAQPGGEAEAAAPA
                     LSAAGLIVANDRQPGAAQSDARSGAGAASVGSGEHQGFEAQPAAADATPAAMAGAEDQ
                     LAEQIAEQASYWVHQKTQNAELTLDQGGRPVEVRVALTGDQAHVSLRSDQLEARQLLD
                     AGREQLQDMLQRQGLQLAGMTVGAGGGHGAGSRQDGREPGRQGAQRASVQAALPVGAA
                     GRGSGVGERSVDIFV"
     gene            complement(16407..16865)
                     /gene="fliJ"
                     /locus_tag="PK518_06855"
     CDS             complement(16407..16865)
                     /gene="fliJ"
                     /locus_tag="PK518_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRM48671.1"
                     /translation="MGSLNAFVVAVDLAERQRDAARQTLQNLQGARQAAQAQLEQLSG
                     YAAETQQRWGMREGAAVQPEVMRHHYQFMGRLDHAIGLQTQAVSGQDQRVHQAGQVLL
                     QAELRLASLRKVLERRRADLLREQQRREQKQTDELASQRAAARAGAVHQE"
     gene            complement(16871..18271)
                     /gene="fliI"
                     /locus_tag="PK518_06860"
     CDS             complement(16871..18271)
                     /gene="fliI"
                     /locus_tag="PK518_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HRM48672.1"
                     /translation="MPIEPASAWGEFMAAARARVAQPQALEARGTLTRLTGLVLEAAG
                     LRVPVGAQCQMRMPGQEPVLAEVVGFAGDKAFLMPAGDIHGLSSGASVTPAAPYVPAP
                     RLGAAGGESLDTSAGVLRLPVGAGLLGRVVDSQGQPLDHGGPLAGVTALPMDRSPINA
                     MERDPVREPLDTGVRAINALLTVGRGQRLGLFAGSGVGKSVLLGMMARYTRADVIVVG
                     LIGERGREVKEFVEDILGAQDRGRSVVVAAPADASPLLRMQGAAYATAIAEHFRDQGL
                     HVLLLMDSLTRYAMAQREIALAIGEPPATKGYPPSCFAKLPALVERSGNGLNGVGSIT
                     AFYTVLSEGDDQQDPIADAARAILDGHIVLSRALAEGGHYPAIDIEQSASRVMHNVVQ
                     REHFDMARSFRAIYSRYQKSRDLIQVGAYMSGSDPQLDEAIRLQPGMVGFLQQSMFEA
                     APMDDCLAAMGQVLEG"
     gene            complement(18261..>18753)
                     /locus_tag="PK518_06865"
     CDS             complement(18261..>18753)
                     /locus_tag="PK518_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HRM48673.1"
                     /translation="QARLAAQQQLDDYVAGQGREAAERLAQVAQSLQDSLAGLQQAMA
                     QEVLQLACDIARQVVRREVAGNPQAMLPVVREALGMLAAESRPATVRLHPDDWAALEK
                     PLREEFASPRIEWQADAAVPPGDCLVESAGMQIDGTLDKRWRRAIAALGLTAAWREDG
                     HAD"
BASE COUNT         3155 a         6410 c         6079 g         3009 t
ORIGIN      
        1 ttgaagtata catcgggttt taaccatctt gcaactcaag gtgggaaaat tttctcttcc
       61 cattcacttc gaccggtgca actcagtgag agcccttgtc tgcagcgaag cgtgaagtat
      121 agcgcgggtt tacccctaaa aatcccaaac cgcagctttt ttgcgcagag cgtcaccgtg
      181 cgccctgcgg gggcactgcg accaccgccg ataatttcgg ccatggcatt gattactttg
      241 atggaagccc aactggcgtt cgggcatgta gccctgctgg atcacgcaag ctttgcgctg
      301 gagcccgctg aacgtgtggg cctcataggg cgcaacggag cgggcaagtc gtcgctgctg
      361 aagatcctgg gcggactggc aaaggcggac gacggcagcc tgagtgtgca gcagggtttg
      421 cgcatcacct atgtggcgca ggagcccgag cttgaccccg aggccacggt gttcgcggcg
      481 gcggcacaag gcattgcgca ggccattgcg ctgcgcgagc aatacttgtc cggagcccca
      541 ggattggatc tggatgcgct gcaatcgcag atagaagcct atgacgcctg gaactgggag
      601 cagcgcgtgg aggaaaccct gcagcgcctg caccttgacc cacaggcgct gataggcacg
      661 ctgtcgggcg gcaccaagaa gcgcgtggcc ctagcgcagg ccctggtggc acggcccgac
      721 gtgctgttgc tggacgaacc caccaatcac ctggatctgg actccatcga gtggctggaa
      781 gaactactcc tggccttcaa gggcagcgtg gtcaccatca cccacgatcg cgcctttctg
      841 gatcggatcg ccacacgcat cgtggagctc gatcgcggac agctgcgttc ctacccgggc
      901 aacttcgctc aataccaggc acagaaagaa gagcagctgg cgcaggaggc cgtgatcaac
      961 gcgcgtgctg acaagctgct ggcacaggag gaggtctgga tacgcaaggg cgtggaggcg
     1021 cggcgcacgc gcagccaaag ccgcatcggc cgcctggagg cgctgcgtgc caaccgtgcg
     1081 gcgcggcgcg aggcgctggg cagcgtgagg atggacatcg cctcgggcgg ccagggcggc
     1141 taccagggca agatcgtggc cgagctcacg cgtgtgagca aggcctttgg cgacagggcg
     1201 atagtgcgcg atttcagcgc caccattttg cgtggcgaca aggtggggtt gattggcccg
     1261 aacggcgccg gcaagaccac gctgctcaag ctcatcctgg gcgagctcac acccgatagc
     1321 ggcgacgtgc gccgcggcaa taacctgcag gtggcgtact tcgaccagat gcgcaacgcc
     1381 atcgacctgg acgccacgct ggaagacttc atcagcccgg gcagcgagtg gattgagatc
     1441 ggcaaccagc gcaaacatgt gaagagctac ctgggcgact ttttgttctc gccggcacgc
     1501 gcgcattcgc ccgtgcgcac gttgtcgggc ggcgagcgca accggctgct gctggcgcgg
     1561 ctgttcgcgc ggccggcgaa tgtgctggta ctggacgagc cgaccaacga cctggacata
     1621 gacacgctgg agttgctgga ggagctgctg cagagctacg acggtacggt gttcctggtg
     1681 agccacgacc gcagctttct ggacaacgtc gtcaccagca ccattgcggc cgagggcgat
     1741 ggcctgtggc gcgagtacga aggcggcgtg caggactggc tgacgcagtc acgccgcagc
     1801 cgcgagctgg ctggcagtgc tgcgccagcc gccaagaata ccagtcaaaa cgaagcaaaa
     1861 cgcataacag acaagcgcga acagctacaa aaaaagaagc ttagctacaa ggaacaacgc
     1921 gaactggagc agttgccggc ccagattgac gcactggagg ccgagcaaac cgccatacgc
     1981 gacgagctcg ccgacggcag cctgtatgcc agagacggca cacgcgccgc cgctttgcat
     2041 gcacgcgatg gcgagatcga ggagctgctg atgcaggcgc tggagcgctg gtccgagctc
     2101 tcgacctgat ggtcgtcaga tgcgatggtg cagccggtcg gtggcgccac gcaggcgcgc
     2161 aatcagcgcc ggcgcaatct gcgtgagcgg cagcacctcg tcggccgcgc catgggcgat
     2221 ggcctcgcgc ggcatgccaa agacgatgca gctggcctcg tcctgcacgt agttgtagct
     2281 gccagcatcc ttcatctcgc gcatggcagc ggcgccgtcg gcgcccatgc cagtgagcat
     2341 gatggcatag gcattgcgac ccagcacggt ggcggccgac ttgaacagca cctccaccga
     2401 gggcttgtgc cggttcacgg gcggaccatc atcgaccacg gccacatagt tggcgccact
     2461 gcgcgcgata tgaaactgct tgccgcccgg tgcgatatag gcatggccgg gcaggatgcg
     2521 ctcgccgttc accgcctcct tcaccgtaat ctgacacagg ctgttgagcc gcgcggcaaa
     2581 gctggtggta aagcccggcg gcatatgctg ggtgatgacg atggccggcg agtcggccgg
     2641 cagatgcacc agtacctcgc gtatggcctc ggtaccgccg gtggaggcac ctatgcagat
     2701 caccttctcg gtggacacgc gacccagcag ggtcgcggcg ctggcgggcg cgggcgctgc
     2761 ctgcgccgtt ccatcagggt gggcagccgt ggtggcagcg cgcaccggca tgcggcgcac
     2821 atgggcggcc gccgccacgc ggatcttctc cacgatctgc gtggccagct cgttcaggcc
     2881 gctgctcagg cccacgcgcg gcttggcgac aaagtccacg gcgcctagct ccagtgcctt
     2941 catggtcacc tcggcgccgc gctcggtcag cgttgagatc atcaccaccg gcatgggccg
     3001 caggcgcatc aggcggccca ggaagtcgat gccgtccatg cgtggcatct ccacgtccag
     3061 cgtgatcacg tcgggattct gctcgcggat catctcgcgc gcgatcaacg ggtcgttggc
     3121 cgtgcctatg cactccatgt ccccttggcg gttgatgatc tcggcgagca ggctgcgcac
     3181 cagggccgaa tcgtccacca cgatgacgcg aattttgggc ttcatatcgc tggctttctc
     3241 atctcaaaac agatccacgg agccgccgct ggtggctcgc gccacgctgg cggcgttgcc
     3301 gcgtgcctcc tgcgccgtcg cctctggatg ggcatgggcc aggcgcttga ccatggcctt
     3361 gcccgtcacc ggaaagaaca ccaccttgcg cggatagatg tcgagcacgt cctcggacac
     3421 cacgggtatg cgctcggtgg ccagatagtc ctgcacgaag cgcgtgttgc gctcgcccac
     3481 gttcatggtg gtgaagttgg ccatcacctg gccgccacca aaaattttgg cctgcaaggt
     3541 ctcgcggcgc gcgcccagct tcatcatctc gttgatcaag agctccatgg cataggagcc
     3601 atagcgcccc gaagaatcac cgttatcacc atcgggcaac atgaaatggt tcatgccccc
     3661 gacgcgcatg cggctgtccc acaggcaggc ggcaatgcat gaacccagca ccgtcatgat
     3721 ggccagcggc tcgcgggcga caaaatactc gcctggcaac accttggcgg cattgtgctg
     3781 aaaatggtga tcaaagaaga aaaacgaggc ctcgcccggc ttgcgtgctt gggccttcag
     3841 ttgttgcagg gtctgcgcgg gctgcgcctg cgcctcgccc tccagcgcag tgacgcgtgg
     3901 gatgcgcctg cgctcggtca gcgcgccggg ctgcggaggt ggagccatgg actgggggtt
     3961 catagacgac ctatctaccc aacacgctca tagaccgtct tgccgcgcag ggcaaacagg
     4021 tcgcgcgaat cgctgaagtt ttctgcatga cccacaaaca gcatgccgcc gggcttgagg
     4081 acacggtgca tgcgctcgag cacgcgccgc tgcgtgggct catcaaagta gatcatgacg
     4141 ttgcggcaga agatcacgtc gaaaggctcg cgaaatggcc agtcgtcgcg gatcaggttc
     4201 acgctcagaa aatcgatggg ttggcgcaac tcggggcgca cgcgcgccat gccggcgtta
     4261 tcgcccttgc cgcgcagaaa gaaacgctgc aactgtgact ggctcaggcc cttgaggccg
     4321 tcttgccggt acacgccgcg ggcggccttg gccagcacgt tggagtcgat gtcgctggcc
     4381 gccagtttga acggggcacg cggtgccagc gcctccagcg cggtcatgac gatggaatac
     4441 ggctcctcgc cggtggacgc ggcgctgcac cagatattcc acggtccatt cgggcgcgtg
     4501 cgcagcaact gtgtcagcac ctcgaaatgg tgctgctcgc gaaagaaggc cgtgaggttg
     4561 gtggtcagcg cgttgatgaa ttcctgccat tccggcccgt cgctgccctc cagccagccc
     4621 aggtagtcgt gaaagctggt gtgcccggtc tcgcgcaggc ggcgcgacag gcggctgtag
     4681 accatggcgt gcttgccatc gtgcaggttg atgccggcgc gctggtagat cagtgcctgc
     4741 acgcgcgaaa aatcggtctg cgtccaggca aactcgcgcc cgctcgcgtc ctcatgctcg
     4801 gcattcgccg ccgtgtggtg ctgaccgacg cgcacatggg cacggcgtgc cgttggcgtg
     4861 gctatggctt gtcggtgcgc gtgcatcagc ttgctgctgt cgccagcccc atgtccacgc
     4921 ccgccatcag gcgctcgatg tccagcagga tgagcatgcg ctcgcccacc gagcccaggc
     4981 cgcgtatgca ctgcgcgtcg atactgcttt ccaggtctgg ggcggggcgc agctgctcgg
     5041 gcgccagctc catcacatcg ctcaccgaat ccacgacgat gcccacgacg cgctcacgca
     5101 ggttcaggat gacgaccacg gtgaagctgt cgtagtccgc ctgcgcacag ccaaaacgca
     5161 ggcgcatgtc cacgatgggg acaatggtgc cgcgcaggtt gatcacgccc ttgatgaacg
     5221 ccggcgcgtg ggcgatgcgc gtgggtgcct cgtagccgcg aatttcctgc accttgagga
     5281 tgtcgatgcc gtactcctcc tggcccaggc ggaaggtgag gtattccctg gcaccggccg
     5341 caacgatctc tatgcccttg tccaccacat tcatgtcttg actccttgct ttctcggctt
     5401 tgggcacgca tcaatggcgt gcgcggcgca ccagcgcgcc cgtgtccaga atcagcgcca
     5461 ccgtgccgtc gcccaggatg gtggcgccag acacgttggg caccttgcgg tagttggttt
     5521 ccaggttctt caccaccacc tggtgctggc ccagcagctc atcgaccagt accgccacac
     5581 ggctgccgtc ggcctctacc acgaccatga tgttgttgcc actgctaccc gcgccgcccg
     5641 gccggggcac ctggaaaatg cgctccagcg acatcacggg catgtactcc tcgcgcacct
     5701 tcaccagctg cgaaccctgg gtgaccgtgt tcacatcacc ctcgttgacc tggaacgact
     5761 ccaccaccga cgacagcggc aggatgtaga cctcgtcgcc caccccaacc gacatgccgt
     5821 ccatgatggc cagcgtgagc ggcaggcgca ccgacacgcg catgccgtag ccctcggccg
     5881 agtcaatctc caccgaccct cccagcgagg tgatgttgcg cttgaccacg tccatgccca
     5941 caccgcggcc ggacacgtcg gtcaccacat cggcagtgga aaaacccggc gcaaagatca
     6001 actgccagac ctcgctgtcg ggcatggtgt cggagacatc caggccgcgc tcgcgcgcct
     6061 tcttgagaat cttctcgcgc gacatgccct tgccgtcgtc gcgcacctcg atcacgatgg
     6121 agccgccctg gtgcgatgcc gacagggtca gcgagccatg ctcaggcttg cccacggcca
     6181 ggcggtcctc gggcaactcg ataccatgat ccaggctgtt gcgcaccagg tgtgtcaacg
     6241 gatcggtgat cttctcgacc aggctcttgt ccagctcggt ggcctcgcca tgggtgatga
     6301 agtcaacctt tttgcccagc ttcgccgcca ggtcgcgcag catgcgtgga aagcggttga
     6361 agacgatgga catgggaatc atgcggatcg acatcaccga ttcctgcagg tcgcgcgtgt
     6421 tgcgatccag gtccgccagg ccggccagca actgctgatg cgccccggca tccaggccgc
     6481 ggctgttttg cgccagcatg gcctgggtga tgaccagctc accgaccagg ttgatgagct
     6541 gatcgacctt gttcacggcc acgcggatgg tggaggactc catggacgcc tggctggccc
     6601 ccttggcctc cggcgctgcg cgtgccgcgg cggtgcgctg cgcgggggcc gcagcagccg
     6661 ccagcgcggc aacggccact tcatccacag gcacgcccgg agcgccggca aagaaaccat
     6721 agggccgggc cgcatcgggc gcgggcgact gcgccacggg cgctgcagga gcaggcgccg
     6781 ggcacgcccc tgcggccagg atgctgacct tgtccttggc cacatggaac gcgaacagat
     6841 ccagcagatc gtcgttgctg gaggcggttc gcaccgcgaa gcggcgcatg tcggcaacct
     6901 cgcccggcag atcctcgatc tgcccgagcc cggggatgtc gcggaacagc tccttgatgg
     6961 cgtcggcctg ggccagctgc tccagcgggc caatatggat ttcgagcgcg cgcatgccat
     7021 ctgcgcccac ctcgggcacc actgccggag cggccaccgg cgcgggtgca aaagccggct
     7081 gcggcgcaag cacgacggct tgcggcgcca tggccggcgc ctggccggac gccagctcgc
     7141 tgatactgcg caccagatca tgggtggaga tcgcctcgcc ctgcccgccc gcctgatggc
     7201 gcgccagcag gctgcgcgag gcatcggccg actccagcag cacgtccacc atgggcgcaa
     7261 tcaggcgcag ctcgtggcgg cgcaggcgat cgagcagtga ctccatctgg tgcgtgagtt
     7321 cggccacatc ggcgaagcca aaggtggccg cgccgccctt gatggagtgg gcgcagcgaa
     7381 agatgccgtt gagctcttcg tcgttggcag cctccagatc caggttcagc agcatctgct
     7441 ccatctgatc caggttctcg gcggcctctt cgaagaagat ttggtagaac tgcgtcaggt
     7501 cgaaatctgc gcctgcgctc tcttgatggg tgtccgccat ggtgtcggct tcctgtgaac
     7561 tgcgtgggtg tgatggtcgt caggcgccgg gttcagcgga tgactttttg tatgacttcg
     7621 atgagccgca ccgggtcgaa cggcttgacc agccagccgg tggcaccggc ggcgcggccg
     7681 gcctgcttca tctggtcgct ggactcggtg gtcaggatca ggatgggtgt gcccttgtag
     7741 cgcgggtggg cgcgcagcct gcgcgtcagg ccgatgccgt ccaggcgcgg catgttctgg
     7801 tcggccagca ccaggctgac ggggtgggtc tcggccttct ccagggcgtc ctggccgtcc
     7861 accgcctcca ccacgtggta gcccgcgctg gtcagcgtga aggacaccat cttccgcatg
     7921 gacggagaat catcgacggc aagaatggac aacatggaca actccaacga ctcttgattg
     7981 attgaatggg ttcagggcgt gcatggggcc gctggcagtg gttcagaaca gctccaccga
     8041 accggctgcc atctcccgtt gcgccacggg gttggggcga tccggcgcca taccctcggt
     8101 cggcgcggcg tcctcgcctt cctcgccgcc catgacctgg gccgccaggc gaaaggcgct
     8161 ggcctgcagc acgcggctgg tgtgggtgat gagctgggtg gccatgtcgt gaaactgcaa
     8221 ctcggtcacg gcgctgcgca gcgcggtgcg caaggccagc agttcggcgc atccgtcgac
     8281 acgcgcgtcg gtgagcacgg cattcgcctc cccaaaacgg gcgagcaaat tgtccgtggc
     8341 gtggtcgagc aagccctcca gacgatgcag gtcgtgcgcg gccatcagca gacaatcctg
     8401 caaatcggcc gccaccatca cgggaacctg caccgcaggc ccctcgtctg gcgtaataag
     8461 cgttgattgc atcgtttctc catggctgcg ggctacattt ccgcgggcaa catctctccc
     8521 gtccgggcat gccgcccgcc gccggccggc ggattggtac aacttgttcc aatgtgtatc
     8581 ctatgatacc tatttctact gagcttgaac cgctaaaaag ccccatgcgc atactgcatc
     8641 tcgaggattc gaccgccgac catgccctgg tgcgcctgac tctgcgccgc tcgggcgtgg
     8701 cgcacgagat ccggcaggtg gagacgctgg acgaattcac tcagtgggtg atgcaaaaaa
     8761 accacgacct gatcctggcc gactaccgcc tgccgggatt taccgcgctg gatgcctgga
     8821 gcgtggtgga aaagcaggcg ggccacccgc ccttcgtgct gctgtccggc gcgattggcg
     8881 aggcggctgc ggtggacgcc atgcgcctgg gttttgccga ctacctgctc aaggacgaca
     8941 tggcgcgcct gccgcatgtg ctgcagcgcg cgctggaggt ggccgaggcg cgccgcgcgc
     9001 gcgagcaggc ggcagccgag ctggcggcat ccgagcagcg cctggcagac ctggccgaac
     9061 acctgcagac atcgattgag gaggagcgcg cggcgatcgc gcgcgagatc cacgacgaca
     9121 tcggcggcgc actggccgcc gtgaagttcg acctggcctg gctggggcgc cacagcgcgg
     9181 atgacgatca gcgccggcat gccgccagcg ccaccgagat gctgcaacac gccatggacg
     9241 ccagccagcg gctgatgttc aacctgcgcc cgcccgtgct cgatcagggc ctggtggcgg
     9301 ccgtgcgctg gctggccgag tcgttcgagc ggcgcacgcg cgtgcccgtg cgcgtgagtg
     9361 ccagccgcga ggccatggac atgcccccga ccctgcagct ggtggcctac cgcacggcac
     9421 aagaggcgct gaccaatgtg cacaagcatg cccaggccag ccgcgtgcat atcgacctgt
     9481 ccgaccacga gggcgtcctg acgctggagg tgcaggatga cggccgcggc atcgcctccg
     9541 ggatgcggca gaagccccgc tccttcggcc tcaggggtct tgaggagcgt gcgcggcgcg
     9601 ccggcggctg gctggacatc agcagccgtc cgggccaggg cacctcggtc atcatgagcc
     9661 tgccgctaac atccaccgaa cccgacgcag gagaagaatc gtgatccatg tggtgctttg
     9721 cgacgaccat gccgtgctgc ggcgcggcat acgcgacacg ctggccgaat cccccgagat
     9781 ttgcgtgacc ggcgaggccg gcagctacgc cgagctgcgc gaggtgctgc gcgccgccac
     9841 ctgcgacgtg ctgctgctgg acctgaacct gcctgggcgc agcggcctgg aggcgctggc
     9901 cagcctgaag gagagccacc ccgcgatcaa ggtgctgatc gtctccatgt accccgagga
     9961 ccagtacgcg ctgcgctgcc tgaaggccgg cgcccatggc tacgccaaca agggcggcga
    10021 cccggtgcag ctgattgccg ccgtgaagac cgtggtcgag gggcgcaaat acctcacgcc
    10081 cgaggtggcg cagctgctgg ccgacagcct ggcccagccc acgcccgagc tgccgcacga
    10141 gtcgctctcc gagcgcgagc tgcagacgct ggtgaagatc gcctcgggca agcgcctgtc
    10201 ggacattgcc gaggagctgc tgctcagccc caagaccgtg agcgtgtacc gcgcgcgtgt
    10261 gctggagaag ctcaagctgg ccaacaacgc cgagctgacg gtgtacgcca tacgcaaccg
    10321 gttggtatga aaacaatagc tgtttgcgct tgatatacaa gcatttcaac catttttcta
    10381 tttgaccatc ccaattacaa agcgcaaaac gctatcgttt ttggtgaatc aacgcccgcc
    10441 gccaaacaga tccatggact gctgcagcag cgtcagcatc ggctgctcca gcatcggcag
    10501 ggtggccgtg atgcccagca tgcccacggt gagtgtgacc gggaagccca cggcgtagat
    10561 gttcatctgc ggcgccacgc gcgagatgat gcccagcgtc aggttgacga acagcagcat
    10621 ggcaatcatg ggcagggcga tccacaggct gctggcgaac agcgacgcgc ccagctcatg
    10681 caggcgcatc cggcccacgg cctgcagaaa atggccatcc acgggaaagc tgtcaaagct
    10741 cttgaccacc gccatcagca ccatcaggtg gccgttgatg accacgaaca gcagcatggc
    10801 aatgttgccg aagaagcgcg ccacggcgct cacctgggcg ttggccatgg gatcgaagaa
    10861 cgaggcgaaa ttcagcccca tctgcaggcc gatgacctcg cccgccagct ccacggcgac
    10921 gaacaccagg cgcacggcaa agcccagcgc caggcccacg cccacctgct gcagcacggc
    10981 gcccagggcc tcgcggccgt tcagatctat caccggctga tcgggcagca ccgcctgcgc
    11041 acacagcgcc acgaagaagg ccagaccgat gcgcacgcgc accggaatgg cgcgcatgga
    11101 gaacaccggc gccgccgtga acagcgccag cacgcgcaga aacggccaca gcaccggcga
    11161 cagccaggcc atgatctggg cctcggtgaa ggtgatcann nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnta gtccaccagc gtggtgatca tccacggccc ggcgatggtg
    11341 aacaccacca ccgccgccac cagcttgggc acgaaggcca gcgtggcctc gtggatctgc
    11401 gtcacggcct ggaagatgct gaccaccagg cccacggcca tcaccacgcc gagcaccggc
    11461 agcgagatcg tcagcagcag cgtgagcgcg tcgcggccca tggtgagcac gaattgggag
    11521 gtcatgaaaa atcctttgag tcagccgttg tgctaaatgc cggcaacgta taaaactggc
    11581 gcgcgccaag ccagcggccg ccgtgcaagg gccgccccgc cgcacgggcg gcgtccccct
    11641 tcccacgcgc agcgtgagag aagggggaag cgacgaagtc gctcaggggg ttgctactca
    11701 tacagcgaag ctggcggcca gcgagccgat cagcaaattc cagccatcgg ccagcacgaa
    11761 cagcatgagc ttgaacggca gtgccaccag caccggcgac agcatcatca tgccgagcga
    11821 catgagcacg ctagagacca ccatgtcgat gatcaggaac gggatgaaaa ccatgaagcc
    11881 gatctggaac gcggtcttga gctcgctggt cacgaaggcc ggcaccagca cgcgcagcgg
    11941 cgcggtctcg gccgtcacgc catcgtccag cctggccagg cgcgcaaaca gcgcaaagtc
    12001 cgactggcgc gtctgcttga gcatgaagcc gcgcatgggc gcctcggcct tgtccagcgc
    12061 ctgctcgaac gacagcgcgt tggtggtgta gggcacatag gcctcctggt aaaccttgtc
    12121 cagcgtcggc cccatgacga acagcgtgag gaacagcgac aggccgacga ccacctggtt
    12181 cggcggcgcc tgctgcgtgc ctatggcctg gcgcaacagc gacagcacga tgacgatgcg
    12241 cgtgaagccg gtcatcatca gcagcaccgc tggcagaaag gacagcgccg tgaagaacag
    12301 cagcgtctgt atgggcaccg aatagctgtt gccacccgcg cccgagccaa tcaccagcgg
    12361 cagcgtgccc gccgcgccgc cctgctgcgc cagggccacg ccggcccaca gcaaagcgcc
    12421 cagcagcgct gcggcgcgca agcgtttatg catctgcgcc cccatccacc aagggctgtg
    12481 cgcgcgccgc cgccatctcc gcggcaaagc cggtgccatc ggccgccgga gccggcagca
    12541 catgcaggca attgatgttc tgcgccgtca cgcccagcac cagcaccgtg cgctgctgcg
    12601 gcggccccac ctcgacggtc accacgcgct gctgcggccc cacggcgacg gccgacaaca
    12661 cgcgcgacat ggcgcccgcc tgcccgagct ggccggcgcg ctgctgctgc aggcgccgga
    12721 tcagccaggg cagggccgcc agcgccgcga ccagcagcac cagcagcagc aatgtctggg
    12781 tcatgcccat gccatcaacc ccggctgaca cggcgcaggc gctcagaggg cgtgaccacg
    12841 tcggtcaggc ggatgccgaa cttgtcgttc accaccacca cctcgccctg ggcgatcagg
    12901 tagccgttga ccagcacgtc catgggctcg ccggccagcg cgtccaactc caccaccgaa
    12961 ccctgggcca gctgcaggat gtacttgatg ggcaccttgg tgcgacccag ctccaccgac
    13021 agctgcaccg gaatgtccag caccatgttg atgtcctgca cctgggcatt gccattgccg
    13081 ccaaaggggc gcgcgggctc gcccgacagc gggccgccct gatcggcctc cacggcttgc
    13141 gcatcggcgc tcttttgctc ctccagcgcc tcggcccagc cggcaaacgg atcgtcggcc
    13201 gccgcggcgc cattggggtc gttgtgatcg gattcagttg acatgtttct ctcccatcca
    13261 gtgggcctct atgccgcgca ggcattcctc tacgcggatc gcgtacttgg cgttgtgtgt
    13321 gccgtattgg cagtcaaaga cgggcacgcc ctcgatggtc gcgcgtatgc gcggctggcg
    13381 ctccagctcg atgaagtcgc ccggcttcat ggccagcagc tgctcgacgg tggcatccgc
    13441 gcgcgccagc tcggccacca gcgtgacctc ggcggcctgg atctcgcggg tgagcacgcg
    13501 cacccagcgc ctgtccacct cgatggcatc gccctgcgtg gccgaataca gcacgtcgcg
    13561 tatcggctcc agcgtggcgt agggcatgca gatgtggatg gcgccggaca ggtcgccgat
    13621 ctccagctgg aatgccgtgg agaccacgat ctcgctgggc gtggcgatgt tggcaaactg
    13681 cggctgcatc tccgagcgct ggtaggacag ctccagcggg tagatgccct gccaggcctt
    13741 cttgtattcg gcgcagatca cctccaccag gcggttgatg acgcgctgct cggtggccga
    13801 gaagtctcgc ccctcgatgc gcgtctggaa cttgcccacg ccgccgtaca gcgtgtcgat
    13861 gatgccgaac agcagcgagg gctcgcacac gatcaggccg ctgccgcgca gcgggcggat
    13921 ggagacgatg ttgaagttgg tcggcaccac cagctcgcgc agaaaggcgt tgtagcgctg
    13981 caccgacacc gcgcccaccg agatctcggg gctgcggcgt atgaagttga acagcccgat
    14041 gcggaagttg cgtgcaaagc gctcgttgac gatctccatc gtcggcatgc gcccgcgcac
    14101 gatgcgctcc tggctggaga tgtcgtagtc gcggacggcg ccggcctcga tctcctcctt
    14161 gacggttttc tggctttcgc cggtcacgcc ctccagcagc gcatcgacct cttcctggga
    14221 cagaaaggaa tcactcacgg caagcctccc tcaggcggtt cacttactgg atgatgaagc
    14281 tggagaacag cacctggcgc accgggttgt tgtccgcctt ggcgcgccgg ggcgcgacct
    14341 cgtcgtcctc ttcctcgtcc tccgggtcac gctgcaccgg gcgcttgcgc ggcttgggca
    14401 ccgtgaagcc cagcgggcgc gagacctcgc ggcgaatgtc cacggccagc ttttccttgc
    14461 cctcgcgcgt cagcagctcc tcggtgctgc gctgcgacac cagcagcagc acattgctgc
    14521 gaatgctggg catgaaggac ttgacgagct cgccggtctt ggcgtcggcc agctccagcg
    14581 tgatgcccac ctgggcaaaa cggtcaccgc cggggtcggc caggttgacc accatgttct
    14641 ccatgggcag aaaggtgggc ggcgtcttgg gcgcggccgc ctgggccacg ggcgcatgcc
    14701 cctcctcctc gtcaccgccg ccgcgtttgc ccagcagcag ccaggcggcg gcgccgccgg
    14761 ccaccagcag cacggccagg gccgccacga tgatgatcag tttcttgctc ttggccttgg
    14821 ccggggcggc agtgggttcg gacacgttgg gactttcttg cgcgcgggcc gcgtcagaca
    14881 gtggagccgc agacagcatg cctggggccg ctgcctgttc gatgccccat tattcagccg
    14941 ccgcccctgc cccgatcgcc ggaataaaag cccaaaaccc cggctatcgg gcgcttgcgc
    15001 cgctcacaca aagatatcca ccgagcgctc gcccacgccc gatccacggc ccgcggcgcc
    15061 cacgggcagc gccgcctgca cgctggcgcg ttgcgcgccc tggcggcccg gctcacggcc
    15121 gtcctggcgc gaacccgcgc catggccgcc accggcgccc accgtcatgc cggccagctg
    15181 caggccctgg cgctgcagca tgtcctgcag ctgctcgcgg ccggcgtcca gcagctggcg
    15241 cgcctccagc tggtcactgc gcagactgac atgggcctgg tcgccggtga gcgccacccg
    15301 cacctcgacc ggtcggccgc cctggtccag ggtcagctcg gcgttctggg ttttctggtg
    15361 cacccaatag ctggcctgct cggcgatctg ctcggccagc tggtcttcgg cgccggccat
    15421 ggcggccggc gtggcgtcgg ccgctgcggg ctgcgcctcg aagccctggt gctcgccact
    15481 gcccacggac gcagcgcctg cgccagaacg cgcgtcggac tgtgcggcac caggctggcg
    15541 gtcattggcc actatcaaac ccgcggcgga cagggccggc gcagccgcct cggcctcgcc
    15601 acccggctgc gccaggggca tgggctgctg ggcaccacca cgctcgctga tggccaagcc
    15661 tgtggtctgt gctgcggcag gcatcgtcac tatcgattca gtagctgttt gcgcaatgga
    15721 tacaacacct gcggcgttat ttcgtcctga tccggcagct cccgatgggc catcggccgg
    15781 ctgccatggc tgtgcgcgcg ccgccacacc acgccccgcc ggcgcagccg cgcccatgcc
    15841 ctggctagta ccgcccagcg cggcagcctc tgcggcgccg tccagcatgg cggtttcgcc
    15901 caccaggctg ccgctcgtcc agcgctgcgt accggcctgg gccacgccca tcaggccctg
    15961 accactgcgg ccggccaggc ccgacagggc cgccacctcg ccccccgtgg tggcagctgc
    16021 gccatccata acgacgcccg caccctgcgc ctgcacagcg gtgggctgca ggcccagcat
    16081 ccagggcgcc agcgctgcgc catccggcat gcccggctgg ggcttcagcg cctgctcacc
    16141 ggcttcgggt gccgtggtgc ccaggccatc gagcagcgcc accgcgccgc cgacctgttc
    16201 gccggcctgt tctcctgcct gcgtgctggc ctgttcgctg gcgccaccgc ccagggccgc
    16261 cagtagcaag gcaaaaccgc cgccctggcc cgcggcgatg gcggcgtcct cggcactgcc
    16321 gggtttttgc cccgtgccgc gcgcggcgcg cgcctcatgc gcgctctgcg gcgccggggt
    16381 ggctatgcgt gtcttttcca tggctgttac tcctgatgca ccgcgcccgc cctggccgcg
    16441 gcgcgttgtg atgcaagttc gtcggtctgc ttttgctcgc gccgctgctg ctcgcgcaac
    16501 aggtctgcgc ggcggcgctc cagcaccttg cgcaggctgg ccaggcgcag ctcggcctgc
    16561 agcagcacct gcccggcctg gtgcacacgc tgatcctggc cgctgacggc ctgcgtctgc
    16621 aggcctatgg cgtgatcgag ccggcccatg aactggtagt ggtggcgcat gacctcgggc
    16681 tgcacggccg cgccctcgcg catgccccag cgctgctgcg tctctgccgc gtagccactc
    16741 agctgctcga gctgggcctg cgccgcctgg cgtgcgccct gcaggttctg cagcgtctgg
    16801 cgcgccgcat cacgctggcg ctcggccaga tccacggcga cgacaaaggc gttcagcgag
    16861 cccattggct tcagccctcc agcacctggc ccatggccgc caggcagtca tccatgggcg
    16921 cggcctcgaa catgctttgc tgcagaaaac ccaccatgcc cggctgcagg cgtatggcct
    16981 cgtccagctg ggggtctgag ccactcatgt aggcgccgac ctggatcagg tcgcggctct
    17041 tctggtagcg cgagtagatg gcgcggaagc tgcgcgccat gtcgaaatgc tcgcgctgca
    17101 ccacgttgtg catcacgcgc gaggccgact gctctatgtc tatggccggg tagtggccac
    17161 cctcggccag cgcgcgcgag agcacgatgt ggccatccag aatggcccgc gccgcatcgg
    17221 caatcgggtc ctgctggtcg tcaccctcgg acagcacggt gtagaaggcg gtgatcgaac
    17281 ccacgccatt gagcccattg ccgcttctct ccaccagcgc cggcagcttg gcaaagcagc
    17341 tgggcggata gcccttggtg gccggcggct cgccgatggc cagcgcgatc tcgcgctgcg
    17401 ccatggcgta gcgcgtcagc gaatccatga gcagcagcac atgcaagccc tgatcgcgga
    17461 aatgctcggc gatcgcagtg gcataggccg cgccctgcat gcgcaacagc ggcgacgcat
    17521 cggccggcgc ggccacgacc accgagcggc cgcgatcctg cgcgcccaga atgtcctcga
    17581 caaactcctt gacctcgcgc ccgcgctcgc caatcaggcc gacgacgatg acatcggccc
    17641 gggtgtagcg cgccatcatg cccagcagca ccgacttgcc cacgcccgag ccggcaaaca
    17701 gccccaggcg ctggccgcgt cccacggtca gcagcgcgtt gatggcgcgc acgccggtat
    17761 ccagcggctc gcgcaccggg tcgcgctcca tggcgttgat cgggcttctg tccatgggca
    17821 gtgccgtcac cccggccagc ggcccgccat ggtccagcgg ctggccctgc gaatccacca
    17881 cgcggcccag caggcccgcg ccaacaggca ggcgcagcac gccggcgctg gtgtccaggc
    17941 tctcgccgcc ggccgcaccc agccgcggcg ccggcacata gggcgcggcc ggtgtcacgc
    18001 tggcgccgct ggacaggccg tggatatcgc ccgccggcat caagaaggcc ttgtcgccgg
    18061 caaagcccac cacctcggcc agcaccggct cctggcccgg catccgcatc tggcactgcg
    18121 cgcccactgg cacacgcagg cccgcggcct ccagcaccag gccggtcagg cgcgtgagcg
    18181 tgccgcgcgc ctccagcgcc tgcggctgcg ccacgcgcgc gcgcgccgcg gccatgaact
    18241 ctccccaggc gctggcgggc tcaatcggca tggccatcct cacgccaggc ggccgtcagg
    18301 cccagggcgg cgatggcgcg gcgccagcgc ttgtccagcg tgccgtcgat ctgcatgccg
    18361 gcggattcca ccagacagtc gccgggcggc acggcggcgt ccgcctgcca ttcgatgcgc
    18421 gggctggcga attcttcgcg cagcggcttt tccagcgccg cccagtcgtc cggatgcagg
    18481 cgcaccgtgg ccggccggct ctcggccgcc agcatgccca gagcctcgcg caccacgggc
    18541 agcatggcct gggggttgcc tgccacctcg cggcgcacca cctggcgtgc gatgtcgcag
    18601 gccagctgca gcacctcctg cgccatggcc tgctgcaggc ctgccaggct gtcctgcagg
    18661 ctttgcgcca cctgcgccag gcgttcggcg gcctcgcggc cctggccggc aacatagtcg
    18721 tccagctgct gctgcgccgc caggcgcgcc tgc
//
LOCUS       DAOMTQ010000050        18658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_397_length_18658_cov_22.705316, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000050 DAOMTQ010000000
VERSION     DAOMTQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 18658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18658
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_18658_cov_22.705316"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2338)
                     /gene="prsT"
                     /locus_tag="PK518_06870"
     CDS             complement(<1..2338)
                     /gene="prsT"
                     /locus_tag="PK518_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HRM48674.1"
                     /translation="MPISRSAFPSATLAIALLVSACGGDNPDTLVTSAQDYLNRQDAP
                     AAIIQLKNALKERPDFIKARLMLGQALLATGDAQGAETEFKKAQDLGAPADEIVPQLV
                     QALMQARQFDKVTNDYASKQLSGEEAQANLKTNVAIAWQRQGQQDKARESLNEALKAK
                     PDYAPALLELARTSALSGDIEAALVGLDKVPRQSAAGAEALKLRGDLLLYSKRDMAGA
                     LAAYQESLQVNPAYKEGQAAIIELLLVQGKVDDAEKALQVLVKEAPGRVPTLYLEAML
                     AYTKKDFKTTQEKIQNLLRLAPESYRGFELGGMAELQLGSMVQAEAMLSKALQLNPGL
                     AMARRGLVTTNIRLGRLDNAVALLPEEPKDGKDGNSDPALLALAGQVYMLRGDIDRAQ
                     RYFARASSLDPKDPAKRTSLALSHLAAGQGDTAIGELQNIAASDEGVVADMALINTLL
                     RQGKTDQALKAIDALQKKRPADVVPPFLRGRALLQKGDKAGARKAMEQALQIDGNYFP
                     AVGVLAVIDNAEKRPDEARARLEAAIKQQPANIQAYQLLLELRAAHGADKAELTGILR
                     RAVDGAPNNPVPRQMLAEHYLRTGEPKEALTVAQNATAALPDNMQLLDVLGRAQLANG
                     EHNQAQSSFNRMAALQPKSPLPYLRMSSAHLVAGDQAAAAEKLRKALEITPNLLQAQQ
                     GLASLAMAANKPDEALAIARTVQKQRPKEAAGYILEGEIQAAGKAWDKTIDAYRAGLK
                     QVDNPELAVRLHSALQSAGKKADADRWAADWTRSHPKDA"
     gene            complement(2377..3738)
                     /gene="prsR"
                     /locus_tag="PK518_06875"
     CDS             complement(2377..3738)
                     /gene="prsR"
                     /locus_tag="PK518_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HRM48675.1"
                     /translation="MSAEKLQPLLVVEDDLALQKQIKWSLDGYESVLANDRESAMAQL
                     RRHGSPVVTMDLGLPPDADSVSEGFKLLEQILAAAPDTKVIVLTGQNGQANALKAVAM
                     GAYDFLAKPFEPEVLNLCVERAFRMHELQAENKRLQALQASDAIAGLITRDPQMLRVC
                     RTIEKVANTNASVMLLGESGTGKEVLARGLHQQSNRSNGSFVAINCAAIPENLLESEL
                     FGYEKGAFTGAAKTTPGKIETAHGGTLMLDEIGDMPMPLQAKLLRFLQERVVERVGGR
                     QGIAVDVRVVCATHQNLAQCIKDGRFREDLYYRLAEIVVEIPPLRQRVGDAALLAHAF
                     ARRFAQEHGRSLTLADDALRAIEAHPWPGNIRELENRIKRATIMADGSQITALDVGFT
                     DADGVDDDRSLDLRVIREAAEQRAVLAALARTDGTVAKAAELLGVSRPTLYDLMHRLG
                     LKT"
     gene            complement(3751..5832)
                     /gene="prsK"
                     /locus_tag="PK518_06880"
     CDS             complement(3751..5832)
                     /gene="prsK"
                     /locus_tag="PK518_06880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HRM48676.1"
                     /translation="MNGSESTVVYWGWALAAGAYSLMSLRLIGQQYLGAAVNRIAVVM
                     LAASVLTVIWSVSSLLALTESSAWWLGAQAADILRYLCWTVFVALFFRTNAGQANDGW
                     YRWWPGLVVVGLFGAPAAVVMIYLLAGAKGQAFLMVLLAIAGLVLLEQLLRNVPEDSL
                     WSAKPVCLGLAGTFLFDLYVFSQGVLFQGIDPDALSVRPFVHALMVPLLWLATTRHRN
                     WIAKIHVSHKVMFHSATLVLVGLYLLFMASVGYYVRYFGGAWGGALQLGLVFVAVVLA
                     IGVALSGSLRATLRVFLGKHFFRYRFDYREEWLKFTATLSSQDHPQEAGKNVIRGLAD
                     MLESPAGGLWLLRPDDDQYRQAARWNLPAATETVDKDGALPAFMRSTAWVVNLEEWRA
                     YPGRYQHLRVPDWLAEYPQAWLLVPLWHGKDLLGFVLLASPRTPVEVNWEVIDLLKTA
                     GRQAASILAQMQATEALLESRKFEAFSRMSAFVVHDLKNIVAQLSLMVKNAKRLQNNP
                     EFQADMLMTVENSLERMRQLMLQLREGASSGAAAVGVDLGKIAERLAANALRRGRTVE
                     VDVSSQVFTRGHADRLERIIGHLVQNALEATGATDRVWIRVDRYGSHARVEVGDEGHG
                     MSEEFVQTRLFKPFQTTKEAGMGIGTYESFQYVQELGGKLSVDSQVGKGTVVGLLLPL
                     IEISRDSDLHL"
     gene            complement(5931..>6239)
                     /locus_tag="PK518_06885"
     CDS             complement(5931..>6239)
                     /locus_tag="PK518_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HRM48677.1"
                     /translation="RGQVFLVTDGGMHHHLAASGNFGQVIRRNYPVAIGNRLQSAERE
                     SASVVGPLCTPLDMLADKLELAVAQPGDLVVVFQSGAYGATASPQTFLGHPACVEVLV
                     "
     assembly_gap    6240..6339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6340..6978)
                     /locus_tag="PK518_06890"
     CDS             complement(<6340..6978)
                     /locus_tag="PK518_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HRM48678.1"
                     /translation="MDRFDASGAELRVGGLPLSRLAERVGQTPFYAYDRSLIAARIAA
                     VRLVLPPGVRLHYAIKANPMAALVGFLCPLVDGMDVASAGELKLALDAGADPLSIGFA
                     GPGKRAAELRQAVASGVLLHVESARELQLLAKAAQDLGMPARVALRINPDFELRGAGM
                     HMGGGAKPFGIDAEQVPAMLRSMHQDGVVFEGFHAYPGSQNLRGEVIAESLRR"
     gene            complement(6965..8602)
                     /locus_tag="PK518_06895"
     CDS             complement(6965..8602)
                     /locus_tag="PK518_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HRM48679.1"
                     /translation="MSYAPHLTSAIPPVLLHQLPQRAAVCWPTRTALTVEGQHLSYAD
                     LQAQIERCAAGLQQLGLERGARVVLYLDKRVETVVASFAAPAAGAVLVPVNPLLKPTQ
                     VAHILQDAGAQVLITSAARLASLAPVLGDCPELRHVLLCDGEVPPAQAPLPAGIALHA
                     WRAVLATLPAALPRVLDGDVAMIFYTSGSTGLPKGVVLSHRNLVAGATSVASYLRNHA
                     DDTLLAVLPLSFDAGFSQLTTAFLVGARVVLLNYLLPRDVLQAMARERVTCLTAVPPL
                     YMQLTALDWPDGAAQHLRYWANTGGRMPRATLQRMRALAPGALPYLMYGLTEAFRSTY
                     LPPDEVDRRPDSIGRAIPNADVRVLREDGTECAVDEPGELVHRGALVAQGYWRRPDET
                     ARRFKPWPAALLAATGDWAAPERAVFSGDTVRRDAEGFLYFVGRRDEMIKTSGYRVSP
                     TEVEEVLYASGLVAEALVYAKPDEALGSAICAALWASPAASGQAAQDDAALMAHCRQH
                     LPVFMLPRVLQWVDKPLPRSPNGKLDRQRWMQDHGSL"
     gene            8982..9242
                     /locus_tag="PK518_06900"
     CDS             8982..9242
                     /locus_tag="PK518_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM48680.1"
                     /translation="MNLTTQVIRVLDEALGLQGRSAGFQRDTPLLGALPELDSMAVLA
                     LITGLENHFGITFSDEDLHGAVFATVGSLCDLVEQALAQQDT"
     gene            9239..10087
                     /locus_tag="PK518_06905"
     CDS             9239..10087
                     /locus_tag="PK518_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HRM48681.1"
                     /translation="MSPSARAALPQAFFLPGDNGQRFCLYHPPQGSSPQGRVLYLHPF
                     AEELNSTRRVVAQQARALAQAGYAVLQIDLLGCGDSSGDFADATWAAWLQDAQQARRW
                     LLEHGSGPLWLWGMRAGALLAAQLAQQPQEGGDPTHLLLWQPVASGQQQLQQFLRLQA
                     ASQWLGNASPGQTPAQALALGESVHIAGYTLSANLARGLGDARLTPPTAGPAGRLVWL
                     EVSSTQPPQLPPASAKPLAAWSGAGWRVQAQAVNGPAFWQTVGTDDAPALIAATLAAL
                     TDIPPP"
     gene            10084..10926
                     /locus_tag="PK518_06910"
     CDS             10084..10926
                     /locus_tag="PK518_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HRM48682.1"
                     /translation="MNVQEQPLQIAGDGFDMLGILSLPAPGTPTLRTAVLIVVGGAQY
                     RAGSHRQFVLLARRLAAAGYPVLRFDLPGMGDSPGDPVPFENTAPDIAAALDALAQAA
                     PVDRSVLWGLCDGASASLLYLQATQDPRVAGVALLNPWVRSEAGLARAHVKHYYRQRV
                     LQADFWRKLAGGRVGWAALRGIWANLRAMRQTAPALLSFQELMALCWQAFEGKIMLIL
                     SERDLTAQEFIEYASNHAQWRPALQRPGVTRHNLAGADHTCSQPGQQDQLEQLTLHWL
                     ATLV"
     gene            complement(11191..12375)
                     /locus_tag="PK518_06915"
     CDS             complement(11191..12375)
                     /locus_tag="PK518_06915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM48683.1"
                     /translation="MAPFAAEAAPTSRWLRALSEPHFGQAWSRFVAGTPPQGGRSGAN
                     VAGPGITGGVRMYGCEGRRVTAEVFQTEAWFANLLAHGFERPPHALKTWSLGAPSPDG
                     QVGLPLMRLQPGGPLLGLSNYYSCLYGPVGSGKALAGLSAAQWREAAGALRRWPDAGV
                     LRLQPLDADSAWLAALQQALRGAGYWTDRFFCFGNWFQPVPAGGFSAYWAQRPSMLRS
                     SVERGRRRLDRKGAWHVEIHSNDPQGVERALQAYLTVYAQSWKNPEPCPDFMPGLVRT
                     AAREGWLRLGVLWLQDQPLAAQVWLVHGAKANIYKLAYVKGQEKLSAGSALTAALMQH
                     VMDVDRVSEVDYLSGDDAYKRDWMAQRRERVGLLAFNPRRPQGLLTAARHFVGAWLRR
                     RS"
     gene            13267..15345
                     /locus_tag="PK518_06920"
     CDS             13267..15345
                     /locus_tag="PK518_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48684.1"
                     /translation="MANVDLNSSLATPRPPTAATPTPTPTLPPPSVVQPAGAFNCAAD
                     AITCVEVASASSQTQASVPVTFGQPFKAGDWQPTSQGLVAKVEGTTIPLQTDELSTHR
                     DGSARFAVLSAQLTNLPAGQKRIINLYRGAKSNSTPNVPAKPDWNLELEAQVYDANGS
                     VTTLVAQPQAQLVSQIASGSERRLAGAIASEYTVTLPFKNKATGATHPHLTARLHTRL
                     VDGGDRIRTDVVMENTRTWAAAPSNITYSLAIKRNGSTLHTQPQFTHYHHARWHKVIW
                     TGAATEPMARVRHNMPYFIASKAVWNYDLGIKIPEYILSQFYNQYQKSKTAQTNLGPM
                     ANLLMTTAFPNTGGRPEIAPLPQWTAMYLISQDDRMREVMLAHADVAAAVPIHYRDES
                     TVNQPLDLDRYPKVATYLGTSQPALPAVINGITIWKPDTAHQGSYTYVPYLITGDAFF
                     LDEMMFWASWNMTKNIPEYRDFGQGLLRDDQVRGQAWALRALGEVSHALPDSHILKSY
                     FENRLSNNLEWFYKNWIATPIGNVWPMGSPVNPYSYDTVAPWQNDFWAIVMAQLVEAG
                     EPRAKELLTWSSKFGVGRFLNEAQGMCLAKAPGYFLKTKNSSKSWVSSWTEFAQVNFP
                     GVTCTSDLKYDGYPASGTGYVATARAMLATASSLDIPDAAKAYVLWKSKTPLVDKDYP
                     NNPAWAIIPR"
     gene            complement(15688..16869)
                     /locus_tag="PK518_06925"
     CDS             complement(15688..16869)
                     /locus_tag="PK518_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRM48685.1"
                     /translation="MIPVPSSRFLLYGAVAACPELVKSGVKMQLKLCLDRIRYKVGLM
                     GRVSYKNYAEFNAFNRGDNAIVVATQQWVKKTFPDRQIFLVDWGMVNSVKPEGCDKLV
                     VCGSGYFFPNSQGRLSARVADDLEWVKKSGTELHFLGVGFNYLLKWPNLQLAADSSAI
                     VRDMLELSSTITVRDRNTQEFLRQFTSKPIRIIGDPALFIDVARRTDRSLINGRRLRV
                     GVNVPFHGPNATEWIKKNLKKLIATLKIIRNKVGCDFYYFVHYGAEVLVAKLIKDSGI
                     PLTVVNSSAVDLPNEYVNIDMHIGGMLHSCILATAAGTPSIGLAYDHKHHGFFELMDS
                     SAYCIDASSFDAEVLLHKVFLLLENIDAQTEKIKRRREELESSFREAVSIIGGDASDL
                     R"
     gene            complement(17009..18619)
                     /locus_tag="PK518_06930"
     CDS             complement(17009..18619)
                     /locus_tag="PK518_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRM48686.1"
                     /translation="MQGRLIKQLGGGSLLDALLFGATEGVTAAFGVVSAALFVWLLWM
                     FLKRNTAKNSVGTHWTPMRSTWVLAATLLVLEFAVPRLWSNAAYGLGRTLIWGITTNA
                     LDAATDFDKDGYGIFGTQFDSHPFDVTKHPFAVKVVGGKIEIDGFDDNLLIENVPAAR
                     GPMRIPKGAPNLIVVVMESTRGDVIGKRIDGKVVAPNLEALAKTGSVIVPNYSHVGFT
                     TNSLKSMFTGKLEPNMGDPSLFSDLKSSGYRIGVLSGQPEDFGDISKVLQMRKNSDVY
                     VDAETLKDKRAFNFAAKGSLLIDERFLLDAFDREFGSRDKWNQPVFLYFNFQSPHFPY
                     DHPGVAAQVAGAPIPREEINKANQDKVVRTYWNAVANSDAWLGKLMNRLKEIGVWDDT
                     LLLVLGDHGEELFEGGFLGHGHIINDLQYATFFVANRKGVVSGSPMAISDLRDIALDA
                     LSGHAHKEDARPKFMYIGTLEKPAQIGFVDSNRQFTTFKFDTWEACFLEKKSCSSYHS
                     LHDDYRRRVDLLVQRWVSELLTARGSLD"
BASE COUNT         3927 a         6118 c         5111 g         3402 t
ORIGIN      
        1 tggcgtcctt ggggtggctg cgtgtccagt ccgcggccca gcggtcggca tccgccttct
       61 tgccggcgct ctgcagcgcg ctgtgcaggc gcacggccag ctcggggttg tcgacctgct
      121 tcaggcccgc acgataggcg tcgatggtct tgtcccaggc cttgcccgcg gcctggatct
      181 cgccttcgag gatgtagccg gccgcctcct tggggcgttg cttttgcacc gtgcgggcga
      241 tggccagtgc ttcgtcgggc ttgttggccg ccatggccag gctggccagg ccttgctggg
      301 cctgcagcag gttgggggtg atctcgagcg ccttgcgcag tttttcggcg gcggcggcct
      361 ggtcgccagc caccaggtga gcactggaca tgcgcagata cggcagcggt gacttgggct
      421 gcagcgccgc catgcggttg aagctggact gggcctggtt gtgctcgcca ttggcgagct
      481 gggcgcgccc aagcacgtcc agcagctgca tgttgtcggg cagagcggcg gttgcgttct
      541 gtgccaccgt cagggcttcc ttgggctcac cggtgcgcag gtaatgctcg gccagcatct
      601 ggcgcggtac tgggttgttg ggcgcgccgt cgacggcgcg gcgcagaatg ccggtcagct
      661 cggccttgtc tgcgccatgg gcggcacgca gctccagcag cagctggtag gcctggatgt
      721 tggccggctg ctgcttgatg gcggcctcca gccgcgcgcg agcctcatcg gggcgcttct
      781 cggcgttgtc tatgacggcc aaaacgccaa cggcggggaa gtagttgcca tcgatctgca
      841 gcgcctgctc catggccttg cgtgcgccgg ccttgtcgcc cttttgcagc agcgcgcgtc
      901 cgcgcaggaa gggcggcacc acgtcggccg ggcgtttttt ctgcagcgca tctatggcct
      961 tcaaggcctg atcggtcttg ccctgccgca gcagggtgtt gatcagtgcc atgtcggcca
     1021 ccacaccctc gtcggatgcg gcaatgttct gcagctcgcc tatcgccgtg tcgccctggc
     1081 cagccgccag atgactgagc gccaatgagg tgcgcttcgc gggatccttg gggtccaggc
     1141 tggaggcacg ggcaaagtag cgctgcgcac gatcgatgtc tccgcgcagc atgtacacct
     1201 gcccagccag cgccagcagg gctgggtcag agttgccgtc tttcccgtct tttggctcct
     1261 cgggcagcag ggccacggca ttatccagcc gccccaggcg tatatttgtc gtcaccaggc
     1321 cgcggcgcgc catggccaag ccggggttca gttgcagagc cttggacaac atcgcctcgg
     1381 cctgaaccat ggagcccagt tgcagctccg ccataccacc cagttcaaag ccacggtagc
     1441 tctcgggagc cagccgcaac aggttctgta ttttttcctg tgtcgtcttg aagtcctttt
     1501 tggtataggc cagcatggcc tccagataca aggtgggcac cctgccgggg gcctccttca
     1561 ccagcacctg caaagccttc tcggcgtcat cgaccttgcc ctgtaccagc agcagttcga
     1621 tgattgcggc ctgcccttcc ttgtaggccg gattgacctg cagcgattcc tggtaagccg
     1681 ccaacgcgcc agccatgtcg cgcttgctgt acagcagcag gtcaccacgc aacttcagcg
     1741 cttcagctcc ggcagcggac tgccgcggca ccttgtccaa cccgaccaga gcggcttcga
     1801 tatcgccgga caaggcgctg gtgcgcgcca gctccagcaa ggcgggtgcg taatcgggct
     1861 tggccttgag cgcctcgttc aggctctcac gcgccttgtc ctgctggccc tggcgctgcc
     1921 atgcaatggc cacgttggtc ttgagattgg cctgcgcctc ttccccgctc agctgcttgc
     1981 tcgcgtagtc gttggtgacc ttgtcaaact gcctggcctg catcagagcc tgtaccagct
     2041 gcggcacgat ttcgtcggcg ggtgcaccca gatcctgcgc cttcttgaat tcggtttcag
     2101 cgccctgagc gtcaccagtg gcaagcaagg cctggcccag catgaggcgt gccttgatga
     2161 aatcagggcg ctccttcaag gcgttcttga gctggatgat ggccgctggt gcgtcctgtc
     2221 gattcaggta gtcctgcgcc gatgtaacca gggtatcggg attgtctcca ccgcaggccg
     2281 acaccagcaa ggcgattgcc agggtagcgg acgggaaagc actgcgggaa ataggcatct
     2341 tcatcatcat gttgtccttc acagggaacc ggtttcttaa gtctttaggc ccaagcgatg
     2401 catcaggtcg tacagcgtgg gccgactcac accgagcagt tcggcagcct tggcaacggt
     2461 gccatcggta cgcgccaacg ctgccagcac ggcacgctgc tcagccgcct cgcggatcac
     2521 gcgcaagtcc agcgagcggt catcgtccac cccgtcggca tccgtgaagc cgacatcgag
     2581 tgcggtgatc tggctgccat cggccatgat ggtggcgcgc ttgatacggt tttccagctc
     2641 acggatgttg cccggccatg gatgggcctc gatcgcgcgc agagcatcat cggccagggt
     2701 caggctgcgt ccatgctcct gtgcaaaacg gcgggcaaag gcatgggcca gcagcgccgc
     2761 atcgcccacc cgctgacgca gcggtggtat ctcgaccacg atttcggcca ggcggtaata
     2821 cagatcttcg cgaaagcgcc catccttgat gcattgcgcc aggttctggt gcgtggcaca
     2881 gaccacgcgc acatccacgg caatgccctg gcgtccgccc acgcgctcga ccacacgctc
     2941 ttgcaaaaaa cgtagcagct tggcctgtag cggcatgggc atatcgccta tttcgtcgag
     3001 catgagggtg cccccatgcg ccgtctcgat cttgcccggt gtggtcttgg cggcaccggt
     3061 gaaggcgcct ttttcgtagc caaacagctc gctttcgagc aggttttcag gaatggcggc
     3121 gcagttgatg gccacgaagc tgccattgct gcggttggac tgctggtgca gcccccgggc
     3181 cagcacctcc ttgcccgtgc cgctctcgcc cagcagcatc accgaggcat tcgtattcgc
     3241 caccttctcg atggtgcggc acacgcgcag catctgcggg tcgcgtgtga tcagtcccgc
     3301 gatggcgtcc gacgcctgca aggcctgcag ccgcttgttc tcggcctgca gctcgtgcat
     3361 cctgaaggcg cgttccacgc acaggttgag cacttccggc tcgaagggct tggccagaaa
     3421 gtcataggca cccatggcca cggccttgag tgcgttcgcc tggccgttct gccccgtgag
     3481 cacgatgact ttggtgtctg gcgccgccgc cagtatctgt tccagcagct tgaagccctc
     3541 ggacaccgag tccgcatccg ggggcaggcc cagatccatg gtcactactg gcgagccatg
     3601 ccggcgcaac tgcgccatgg cgctttcgcg gtcattggcc aagaccgact catagccgtc
     3661 gagcgaccat ttgatctgct tttgcagcgc caggtcgtcc tcgacaacca acaagggctg
     3721 caacttctcc gcactcatgc cgtccctctc ctataggtgt aaatcggaat cgcgactgat
     3781 ttcaatcaat ggcagcaaca gccccaccac cgttcctttg ccgacctggc tgtccacgga
     3841 gagcttgccc ccgagctcct gcacatactg aaaactctcg taggtgccaa tgcccatgcc
     3901 agcttcctta gtggtctgaa aaggcttgaa caaccgcgtc tggacaaatt cttcgctcat
     3961 gccatggccc tcgtcgccga cttcgacgcg tgcatggctg ccatagcgat cgacccgaat
     4021 ccagacacgg tcggtggcac ctgtggcctc cagcgcgttt tgaaccagat ggccgatgat
     4081 gcgctccaaa cgatcggcgt gtccacgggt aaaaacctgt gacgaaacgt ccacttcaac
     4141 cgtcctcccg cggcgcaggg cattggcggc caggcgctcg gcaatcttgc ccaggtccac
     4201 gcccacggcg gctgcgccag aagacgcccc ctcgcgcagt tgcagcatca gctggcgcat
     4261 gcgttccagc gaattttcca cggtcatcag catgtcggcc tgaaactccg ggttgttctg
     4321 caaccgcttg gcgtttttga ccatcaatga cagttgggcc acgatatttt tcaggtcgtg
     4381 caccacgaat gccgacatcc ggctgaatgc ctcgaacttg cgtgattcaa gcagcgcctc
     4441 ggtggcctgc atctgcgcca gaatgctcgc cgcctgcctg ccagccgtct tcagcagatc
     4501 aatcacctcc caattgactt caaccggcgt gcgcgggctg gccagcagca cgaacccgag
     4561 caaatccttg ccgtgccaca ggggcaccag cagccaggcc tgcgggtact cggcaagcca
     4621 atcgggtact cgcagatgct ggtagcgccc agggtaggcg cgccattctt ccaggttgac
     4681 cacccaggca gtcgaacgca tgaacgcggg cagagcgcca tccttgtcca cggtttcggt
     4741 agcggcgggc aaattccagc gcgcggcctg gcggtactga tcgtcgtcgg ggcgcagcag
     4801 ccacaagccg cctgccgggc tctccagcat gtccgccagg ccacgaatca cattcttgcc
     4861 ggcttcctgc gggtggtcct gactggacag cgtggccgtg aacttcagcc actcctcacg
     4921 gtaatcaaag cggtaacgaa aaaaatgctt gcccagaaac acccgcagcg tggcgcgcaa
     4981 tgaacccgac aacgccaccc caatggccag caccacggcc acgaaaacca ggcccagctg
     5041 cagggcaccg ccccacgcgc cgccaaaata gcgcacgtag tagccgacgc tggccatgaa
     5101 cagcaggtag agccccacca gcaccagcgt ggcggagtga aacatgacct tgtgcgacac
     5161 atggatcttg gcgatccagt tgcgatggcg cgttgtcgcc agccacagca agggcaccat
     5221 gagggcatgc acgaacggcc gcacgctcag ggcatcaggg tctatgccct gaaacagcac
     5281 cccttgcgag aacacataca ggtcaaacag gaaggtgccc gccagaccca ggcacacggg
     5341 tttggcgctc cacagcgaat cctccggcac attgcgcagc aactgttcca gcagaaccag
     5401 cccggcaatc gccagcaaca ccatcagaaa agcctgcccc ttggcccccg ccagcaggta
     5461 aatcatgacc accgccgccg gggcgccgaa cagtcccacg accaccaggc ccggccacca
     5521 gcggtaccag ccatcgttgg cctgaccggc attcgtcctg aagaacaggg cgacaaacac
     5581 cgtccagcac agatagcgca agatatcggc cgcctgcgcc cccagccacc acgccgatga
     5641 ctcggtgagc gccagcaggc tgctgacgct ccagatcacg gtcagcaccg aggccgcgag
     5701 catgaccacg gcaatgcgat tgaccgcggc ccccaggtac tgctggccaa tcaaacgcag
     5761 ggacatcagg ctataggccc cagcagccag agcccagccc cagtaaacca ccgtgctttc
     5821 actgccgttc atggtgacca gctaccaggc ggatgcgtct gctgctgtcg caggttggca
     5881 ttgatgttga tacattaaga aacaatacca cgtaatgcgc ggttttttgc ttacaccaac
     5941 acttcaacgc aagccgggtg ccccaaaaaa gtctgcgggc tggcggtcgc accgtaggca
     6001 cctgactgaa ataccaccac cagatcgcca ggttgggcca cggccagctc cagcttgtcg
     6061 gccagcatat ccaacggcgt gcacaacggc cccaccacgc tggccgactc gcgctcggcc
     6121 gattgcaggc gattgccaat ggccaccggg tagttgcgcc tgatcacctg gccaaaattg
     6181 cccgaggccg ccagatggtg gtgcatgcca ccatcggtca ccaaaaagac ctgtccgcgn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggcgcaggc tctccgcaat
     6361 cacctcgccg cgcaggttct gcgagcctgg gtaggcatga aagccttcaa acaccacgcc
     6421 gtcctggtgc atggatcgca gcatggccgg cacctgctcg gcatcaatgc cgaacggctt
     6481 ggcgcccccg cccatgtgca tgccggcgcc gcgcagctca aagtcggggt tgatgcgcag
     6541 cgccacgcgg gcgggcatgc ccaagtcctg ggcggccttg gccagcagtt gcagctcacg
     6601 cgccgactcc acatgcagca gcacgcccga ggccacggcc tgacgcaact cggccgcacg
     6661 cttgcccggc cccgcaaatc ctatgctcag cgggtctgcg ccagcatcca gcgccagctt
     6721 gagctcgccg gccgacgcca cgtccatgcc atcgaccagc gggcacagaa agcccaccag
     6781 ggctgccatg gggttagcct tgatggcgta gtgcaggcgc acgcccggcg gcaaaaccag
     6841 ccgcacggcc gcgatgcgcg ccgcgatcaa cgaccggtca taggcataaa aaggcgtctg
     6901 acccacccgc tcggcaagcc gcgacaaggg caggccgccg acccgcaact cggcacccga
     6961 ggcatcaaag cgatccatga tcctgcatcc aacgttggcg atcgagcttg ccattgggac
     7021 tgcgcggcag gggcttgtcc acccactgca gcaccctggg caacatgaag accgggagat
     7081 gctggcggca atgcgccatc agggcggcat cgtcctgcgc cgcctgaccg ctggccgcgg
     7141 gcgacgccca cagggccgcg caaatcgcgc tgcccagggc ttcgtcgggc ttggcataca
     7201 ccaacgcctc ggccaccagg ccgctggcgt acagcacctc ctcgacctcg gtcgggctca
     7261 cgcggtagcc cgatgtcttg atcatctcgt cgcgccggcc gacaaagtac aagaagccct
     7321 cggcatcgcg ccgcacggtg tcgcccgaga acaccgcacg ctcgggcgcg gcccagtcgc
     7381 cggtggccgc cagcaaggcc gcgggccagg gcttgaagcg gcgcgccgtc tcgtcgggcc
     7441 ggcgccaata cccttgtgcg accagggcgc cgcggtgcac cagctcgccg ggctcgtcca
     7501 cggcgcattc cgtgccgtct tcgcgcagca cgcgcacgtc ggcgttgggt atggcccggc
     7561 ctatcgagtc ggggcggcga tccacctcgt cgggcggcag gtaggtggag cgaaaggcct
     7621 cggtcaggcc atacatcaga tacggcaagg ccccaggcgc cagcgcacgc atgcgctgca
     7681 gcgtggcgcg cggcatgcgc ccgccggtgt tggcccagta gcgcagatgc tgcgccgcac
     7741 catcgggcca gtccagggcc gtcagctgca tgtacagggg tggcaccgcc gtcagacatg
     7801 tgacgcgctc acgcgccatg gcctgcagca catcgcgcgg cagcaggtag ttgagcagca
     7861 ccacccgcgc gcccaccaga aacgccgtgg tcagctggct gaaacccgcg tcgaacgaca
     7921 gcggcagcac cgccagcagg gtgtcgtcgg catggttgcg caggtagctg gccacgctgg
     7981 ttgcgccggc caccaggttg cggtgcgaca gcaccacacc cttgggcaag ccggtgctgc
     8041 ccgaggtata gaagatcatg gccacgtcgc catcgagcac cctgggcagc gcagcgggga
     8101 gggtggcaag cacagcccgc caggcgtgca aggcaatgcc ggctggcaat ggcgcctgcg
     8161 cgggcggcac ctcaccgtcg cacagcagca catggcgcag ctcggggcag tcgcccagca
     8221 ccggcgccag gctggccagc cgcgcggccg acgtgatcag cacctgggcc ccggcatcct
     8281 gcaggatatg cgccacctgc gtcggcttga gcagcgggtt caccggcacc agcaccgccc
     8341 ctgctgccgg ggcggcaaag ctggccacca cggtctccac ccgcttgtcc aggtacaaca
     8401 ccacgcgcgc accgcgctcc agccccagct gctgcaagcc ggcggcgcag cgttcgatct
     8461 gcgcctgcaa gtcggcatac gacaggtgct gcccctccac cgtcagggcg gtgcgcgtcg
     8521 gccagcagac cgctgcccgc tgcggcagct ggtgcagcaa caccgggggt atggcactgg
     8581 tgagatgggg cgcgtaagac atgcggccga tgttacggca tcgccctacc cggcccccgg
     8641 caagcattgg cacctgcaca tttttttagt agcgcataac gcttgtttgg cgcgtgcttc
     8701 agctccatcg ccacccttgg tgaagaggag gtgacggggc gcagcgccag cattggtcgc
     8761 cctcacccct gccctctccc gcaagcggga gagggagaaa caaccccaag ggcgcacaca
     8821 tattgcgtgg agcgctatac aaaataggtg cagacaccca tgcccggcaa gcgtgggcgc
     8881 tgagtctcgt ccttggccgg gatgacggtc ggcctctttt cttgcgggtc aatagaatcc
     8941 agccgcagcg cacaagacat tccaccatcg ccgcctccga aatgaacctg accacacaag
     9001 tcatccgcgt acttgacgaa gcccttggcc tgcaaggccg cagcgctggc tttcagcgcg
     9061 atacgccgct gctcggcgcc ctgccagagc tggactccat ggccgtgctg gccctgatca
     9121 ccggccttga gaaccatttt ggcatcacct tcagcgacga agacctgcac ggcgcggtgt
     9181 ttgccaccgt gggcagcctc tgcgacctgg tggagcaggc gctggcccag caggacacat
     9241 gagcccgagc gcgcgcgccg ccttgccgca ggcgttcttt ctgcccggcg acaacggcca
     9301 gcgcttttgc ctgtaccacc cgccgcaagg cagctcgccc caggggcgcg tgctctacct
     9361 gcacccgttt gccgaagagc tcaacagcac ccgccgcgtg gtggcccagc aggcacgcgc
     9421 cctggcccag gcaggctacg cggtgctgca gatcgacctg ctgggctgcg gcgacagctc
     9481 cggcgacttt gccgatgcca cctgggccgc gtggctgcaa gacgcacagc aggcgcgccg
     9541 ctggctgctg gagcatggca gcggcccgct gtggctatgg ggaatgcgcg ccggcgcgct
     9601 gctggccgcg caactggccc agcagccgca agaaggcggt gatccaaccc acttgctgct
     9661 gtggcagccg gtggcaagcg gccagcaaca actgcagcag ttcttgcgcc tgcaggccgc
     9721 cagccaatgg ctgggcaatg cgtcgccggg ccagacaccg gcgcaggcgt tggccttggg
     9781 tgaatccgtg cacattgccg gctacaccct gagcgccaac ctggcccggg gcctgggcga
     9841 cgcccgcctg acacctccca cggcagggcc agcggggcgc ctggtgtggc tggaagtctc
     9901 tagcacacag cccccgcaac tgccccccgc cagcgccaag ccgcttgccg cctggagcgg
     9961 cgccggctgg cgcgtacagg cccaggccgt caacggcccg gcgttctggc aaaccgtggg
    10021 caccgacgat gccccggccc tgatagccgc cacgctggca gcactgaccg acataccgcc
    10081 accatgaacg tccaggaaca acccctgcag attgcgggcg acggcttcga catgctgggc
    10141 atcttgagcc tgcccgcgcc gggcacgccc acgctgcgca ccgccgtgct catcgtggtg
    10201 ggcggcgccc agtaccgcgc gggcagccat cggcagtttg tgctgctggc acgccgcctg
    10261 gccgccgccg gctatcccgt tttgcgcttt gacctgccgg gcatgggtga cagccccggc
    10321 gaccccgtgc cgttcgaaaa cacggccccg gacatcgccg ccgccctgga tgccctggcg
    10381 caggcggcgc cggttgaccg cagcgtgctc tggggcctgt gcgacggcgc gtcggccagc
    10441 ctgctgtacc tgcaggccac gcaggatccg cgcgtggcgg gcgtggcgtt gctcaacccc
    10501 tgggtgcgct ccgaagccgg cctggcccgg gcgcatgtca aacattacta ccgccagcgc
    10561 gtactgcaag ccgacttctg gcgcaagctg gctggcgggc gtgtgggctg ggctgcgctg
    10621 cgcggcatat gggccaacct gcgcgccatg cgccagacag ccccggcgtt actatcattt
    10681 caagagctta tggcgctttg ttggcaagcg tttgaaggca aaatcatgct tattttgagc
    10741 gaacgggatc ttaccgccca ggagttcatt gaatacgcca gcaaccacgc ccaatggcgc
    10801 ccggccctgc agcgccccgg cgtgacacgc cacaaccttg ccggcgccga ccacacctgc
    10861 tcccaacccg gtcaacaaga ccagctggag caactgacac tgcactggct ggccacgctg
    10921 gtgtgaacca cgctgcggcc cgtcgatcca ttcaaacggg ttggccgtca tgccaacgaa
    10981 tgccgggatc cagcacgcag gcaattggaa tctgacccca attaggtcaa gatatcaaaa
    11041 agtagagctg catacgcaat accaacaagc gctagagcca tatttttatc aaaatcaaag
    11101 cgacgcgtgc aatgggcccc ggactccgcc gggatgacgg ttaacccgtt gcggcggacc
    11161 agcaggcagt taattggaat ctgactccaa ttaacttcgg cggcgcagcc aggcgccgac
    11221 aaaatgccgc gcagcggtca gcaggccttg ggggcggcgc gggttgaagg ccagcaaacc
    11281 cacgcgctcg cggcgttgtg ccatccagtc gcgcttgtag gcatcgtcgc cgctcaggta
    11341 atccacctcg gacacgcgat caacatccat cacatgctgc atcagcgcgg ccgtcagtgc
    11401 cgacccggcc gatagcttct cctgcccctt cacgtaggcc agcttgtaaa tattggcctt
    11461 tgccccatgc accagccaca cctgcgccgc caggggctga tcctgcagcc acaacacgcc
    11521 caggcgcagc cacccctcgc gcgcggccgt gcgcaccagg ccgggcataa agtcggggca
    11581 gggctcgggg ttcttccagc tttgggcata caccgtcaag taggcctgca acgctcgctc
    11641 cacgccctgc gggtcattgc tgtggatctc gacgtgccac gcacccttgc gatccaggcg
    11701 cctgcgtcca cgctccacgc tgctgcgcag catggacggg cgttgcgccc aataagccga
    11761 aaaaccaccc gccggcacgg gctgaaacca gttgccaaaa caaaaaaacc ggtccgtcca
    11821 gtaccctgcc ccgcgcaagg cctgttgcaa cgcggcaagc cacgcgctgt ctgcatccag
    11881 cggctgcagc cgcaacaccc cagcatcggg ccagcgccgc agcgccccgg cggcctcgcg
    11941 ccattgggca gcagacaggc ctgccaacgc cttgccagag cccaccgggc catacaggca
    12001 gctgtagtaa ttgctcagac ccaacagggg ccctccgggc tgcagccgca tcaacggcaa
    12061 gccgacctgc ccgtccggcg acggcgcacc cagcgaccag gtcttgagcg catgcggcgg
    12121 ccgctcaaag ccgtgcgcca gaagattggc aaaccaagcc tctgtttgaa atacctcagc
    12181 cgtcactctg cgcccctcac atccatacat acgtacgccc ccggttatac cgggcccggc
    12241 tacattggcg ccgctgcgcc caccttgcgg cggcgtaccc gccacaaaac ggctccaggc
    12301 ctgaccgaaa tgcggttcag atagcgccct aagccaccta ctcgtaggag cggcttcagc
    12361 cgcgaacggc gccattcgcg gctgaagccg ctcctacagc gtcgttaact gaactgtatt
    12421 gggcctaacc agcgacattc cccagaggcc gacattcccc cgcttcatga ggacaacagc
    12481 caccgcccga ccccatgcac ccgctcaacg catggatccc gacagtcgag gggatgacgg
    12541 ctacgttgtc acagacgcag ggcagggagc atccaaaaca tgtcactgag ccaacgccac
    12601 gagccattgc gcgcgccaag cagagatatg gcgctatcaa aaaaatctca aaccaccaaa
    12661 ccatagcgcc caagacgcaa cgggaaagga agtagtacat atgcaacgca ttgtgctatt
    12721 tgtttcatgc acacgtctat caaagcaaca aggtcgtatt tgtgtaccaa accgcatgat
    12781 cctctgaatg caaaaagtgc taaaaccaaa acatccgcgg gtgacatccc tatttatatg
    12841 gatgaacaac cagcagatga gagacacacc tcggcagcac ccagcaagcc ttgatcccaa
    12901 tcatccctgc aacaaaccga aactgaacac ataggatcaa tagacacctt ttgtttcgcc
    12961 agcggcttca caatgtcgaa acaatccccg ctgcgtggcc taccgcaact tgcccctggc
    13021 aacaccggta gccacacgcc ccaaaaaccc gcccgaccgc aacgcccatc ggccacaccc
    13081 tcccatccac cacagcacat tgcccctgct tagcacgcgg gcgccccaaa ccgtacgaac
    13141 tcgatcatca tgagcccttc atcgcgcaag aacaacgcac ccggaagcaa ttaccgtatg
    13201 tctattacat gcacactcgc actggccacc ctcacagcat gtggcggggg cggcggcgac
    13261 gacgtagtgg ccaacgttga cctgaacagc tcacttgcca cccccaggcc accgaccgcg
    13321 gccactccca ctcccacacc cacgctgccc ccaccaagcg tcgtacagcc ggctggcgca
    13381 ttcaactgcg ccgctgatgc catcacctgc gtcgaagtcg cctcggccag ctcgcaaaca
    13441 caagccagcg tgccggtcac cttcggtcag cctttcaagg cgggcgattg gcaaccgaca
    13501 agccagggcc tggtggccaa agtggagggc accaccattc ccctgcaaac cgacgaacta
    13561 tcgacacacc gcgacggatc tgcgcgcttt gccgtcctga gcgcccagtt gaccaacctg
    13621 ccggcaggcc agaaacgcat catcaacctg taccgcggtg ccaagtccaa cagcaccccc
    13681 aacgtacccg ccaagcccga ctggaacctg gagctggaag cccaggtata tgacgccaac
    13741 ggaagcgtga ccacactggt ggctcaaccc caggcccaac tggtgtccca gatcgccagc
    13801 ggcagcgagc gtcgcctggc cggagccatc gcgtccgagt acaccgttac cctgcccttc
    13861 aagaacaagg ccaccggcgc cacgcaccca cacctgacag cacgcctgca cacgcgcctg
    13921 gtggatggtg gcgatcgcat ccgcaccgac gtggtcatgg agaacacccg cacgtgggct
    13981 gcagcaccca gcaatattac ctactcccta gcaataaagc gcaacgggag cactctccat
    14041 acccagccac aatttacgca ttatcaccat gcacgctggc ataaggtgat atggacaggg
    14101 gctgccacgg agccaatggc acgggtgcgc cataatatgc cttacttcat tgcaagcaag
    14161 gccgtgtgga actatgacct tggcataaag ataccagaat atattctctc gcagttttac
    14221 aatcaatatc aaaagagtaa gacggcacaa accaatcttg gcccaatggc caatttgctg
    14281 atgaccaccg catttccaaa tacaggcggg cgtccagaga ttgccccctt gcctcaatgg
    14341 actgccatgt acctgatttc acaggacgac cgaatgaggg aggtgatgct agcacatgct
    14401 gatgttgcgg ctgcggttcc aattcactac cgcgacgaat caactgttaa tcaaccgctg
    14461 gatctggatc gctacccaaa agtagccaca tacctcggga cttcacaacc ggccttaccc
    14521 gctgttatta acggcatcac catatggaaa cccgacacgg cgcatcaagg gtcatacacc
    14581 tatgtacctt acctgatcac gggtgacgca ttctttcttg acgaaatgat gttctgggca
    14641 agctggaata tgacaaagaa tattcctgag tatcgtgact ttggtcaagg tttgttgcga
    14701 gacgatcagg tccgtggcca ggcctgggcc ctgcgtgcac tcggagaagt ttcccacgca
    14761 cttccagact cccatattct taagtcatac ttcgaaaatc gcctctccaa caatcttgaa
    14821 tggttctata aaaattggat agctacgcca ataggaaacg tctggccgat ggggtctcct
    14881 gtaaacccat attcttacga taccgtagcg ccttggcaga atgacttctg ggcaatcgtc
    14941 atggctcaac ttgtcgaagc aggggagccg cgcgcgaaag agctactcac ttggtcgagc
    15001 aaatttggag ttggccgctt tctgaatgaa gcacagggca tgtgcctcgc caaggctcct
    15061 ggatattttt tgaaaaccaa gaattcaagc aaaagctggg tcagcagctg gactgagttt
    15121 gcgcaagtta acttcccggg tgttacctgc accagcgatc taaagtatga cggctatcct
    15181 gcatccggca caggatacgt agccacggcg cgcgccatgc ttgccaccgc atccagttta
    15241 gatattccag acgccgcaaa agcatatgtg ctctggaaat caaaaacacc gctggttgac
    15301 aaggattacc caaacaaccc cgcctgggcg atcattccaa gataatccag caataaagga
    15361 aatcctgcaa aacctccccg cctgtctggt agctgtcgtg acgctgtttg cgctgtccaa
    15421 cctgtggatg gcacgccatc agttgatgca agcgcaagga gggcggggat gagtgcgccc
    15481 gaaaacaggg cgcgggccgc caaaggtggc aaaacgccct tgattggcgg gaaataggcg
    15541 gtggcaactg acggaatcaa cccaaagttg caccatctga aaaacacccc gggcgtagca
    15601 cgtctggaag tgagttattc agggcatcct aaactctaaa cgctcgatcg atcagccaaa
    15661 tcatcccccg ccaaactcgg gggatgctca tcgaaggtca gacgcatccc caccgattat
    15721 tgacactgcc tcccgaaaac tggactccaa ttcttcacgg cgacgcttga ttttttctgt
    15781 ttgtgcatca atattttcga gcaacaaaaa caccttatgc aataacactt cggcatcgaa
    15841 tgaactggca tcaatacagt aggccgaaga atccatcaac tcaaaaaaac catggtgctt
    15901 atgatcgtat gccaacccga tcgaaggggt gccagccgca gtcgcgagga tacaagagtg
    15961 caacatacct ccgatatgca tatcaatatt cacatattca ttcggcaaat ctaccgcact
    16021 actattcacc accgtcagag gaatgccaga atccttgatt aattttgcca caagaacctc
    16081 ggccccgtag tgcacgaagt aataaaaatc acaccccact ttatttctaa ttattttcag
    16141 cgttgcgatc aacttcttta gatttttctt aatccactcg gtcgcattgg ggccgtgaaa
    16201 tgggacgttg actccaactc ttagacgcct gccatttatt aaactccgat cagtccgacg
    16261 cgcgacatct atgaataatg cgggatcacc aataattctt atgggcttcg aagtgaactg
    16321 ccgaagaaat tcttgtgtat tgcgatctct aaccgttatc gtggatgata gctcaagcat
    16381 atcgcgcact attgcactcg aatcagcagc aagttgcaag tttggccatt tcagtaaata
    16441 attaaagcca acccctaaaa aatgaagctc cgttcctgat ttttttaccc attccaaatc
    16501 atctgcaact ctagcagaca acctcccctg cgaattagga aaaaaatagc cactcccaca
    16561 tacaaccaat ttatcacagc cctccggctt caccgaatta accatccccc aatcaaccaa
    16621 aaaaatctgt cgatccggga aagttttttt tacccactgc tgggtcgcaa caacaatcgc
    16681 attatcccca cggttaaatg cattaaattc tgcgtaattt ttataactta ccctccccat
    16741 caatccaact ttgtatctaa tccgatcgag acacaatttt aactgcattt ttacaccaga
    16801 ctttaccagt tcggggcaag ctgccacagc cccatacagt aaaaatcgag aacttggtac
    16861 cggaatcata attagaaatt caaatgaatt ttacagtggc tatcccccgg taaatccggg
    16921 actttcgtta ctaagtaagt aattaataaa tttaattcac aaaaccacat tctggatgcg
    16981 caaagcaggg cggccaagta gcgccaattc agtcaagcga acccctagcg gtcaatagct
    17041 cagacaccca tctttgaacc agcaaatcca cacgcctacg ataatcatca tgcaaggaat
    17101 gataagagct gcaggatttc ttctccagga agcaggcctc ccaggtatca aatttgaatg
    17161 ttgtgaactg acgatttgaa tctacaaatc caatttgcgc aggtttttcc aatgtaccga
    17221 tatacatgaa cttaggccgt gcatcttcct tgtgtgcatg accggaaagc gcatcaagcg
    17281 caatatccct cagatcgcta atcgccatcg gactgccgga tacaacgccc tttctatttg
    17341 caacaaaaaa agtcgcgtac tgcaagtcgt taattatatg gccatgccca aggaagcctc
    17401 cctcaaagag ttcctcccca tgatctccga gcaccagcaa aagcgtgtca tcccaaaccc
    17461 caatttcctt gagccggttc atcaatttac ccagccacgc atcggaatta gcaacagcat
    17521 tccagtaggt tcgcacaacc ttgtcttggt tcgctttatt tatttcctcc cgaggtatgg
    17581 gggcaccggc gacctgtgca gcaacacccg ggtggtcata cggaaagtgc ggcgactgaa
    17641 aattgaagta taaaaaaacc ggctgattcc atttatccct ggaaccaaat tcacgatcaa
    17701 atgcatccag caggaacctt tcatcaatca acaacgaacc cttggctgca aaattgaatg
    17761 cccgcttatc ctttagcgtt tcagcatcaa catagacatc ggaattttta cgcatttgca
    17821 atactttaga aatatcacca aaatcctccg gctgacccga cagcacaccg atccgatatc
    17881 cacttgactt aaggtcactg aacagtgatg gatcccccat gtttggctca agcttccctg
    17941 tgaacattga tttaagagaa ttcgtggtaa acccaacatg gctgtaattc gggactatca
    18001 cgcttccagt ttttgccaac gcctccaggt ttggcgccac gactttcccg tcaatgcgtt
    18061 tgcctattac atcccctcgc gtactttcca taaccacgac aatgagatta ggtgcccctt
    18121 tcgggatacg catcggccca cgcgcagcag gcacattctc aattaataaa ttatcatcaa
    18181 atccatctat ttcaattttc ccacccacaa ccttcacggc aaaagggtgc ttcgtcacgt
    18241 caaacgggtg gctatcgaat tgcgtaccaa atattccata accatcctta tcaaaatcag
    18301 ttgcagcatc cagagcattg gtggtaatac cccatatcaa cgttcttcca agaccgtaag
    18361 ccgcgttact ccataatcgc ggcactgcaa actccaatac aagcaaagtg gcggcaagaa
    18421 cccaggtgga acgcatcggc gtccaatgcg tccccacact atttttagcg gtattgcgct
    18481 ttaggaacat ccacaacagc caaacgaaca aagcagcgct gacgacacca aaagctgccg
    18541 tgacgccctc agttgctccg aagagcagcg catccagcag gctgccgccc ccaagttgct
    18601 tgattagtcg gccctgcaaa attcaccgca acatccaacc cagcgatcca cagcgggt
//
LOCUS       DAOMTQ010000051        18056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_422_length_18056_cov_24.813733, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000051 DAOMTQ010000000
VERSION     DAOMTQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18056
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_422_length_18056_cov_24.813733"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..453
                     /gene="gloA"
                     /locus_tag="PK518_06935"
     CDS             40..453
                     /gene="gloA"
                     /locus_tag="PK518_06935"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HRM48687.1"
                     /translation="MRILHTMLRVGNLQRSIDFYTQVLGMQLLRMSENPEYKYSLAFL
                     GFDGGNPDQAEIELTYNWGVESYDMGTAYGHIALGVPDAYAACEKIKAAGGNVTREAG
                     PVMGGTTVIAFVTDPDGYKIELIQRASEAAGGGLR"
     gene            complement(518..1048)
                     /locus_tag="PK518_06940"
     CDS             complement(518..1048)
                     /locus_tag="PK518_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRM48688.1"
                     /translation="MSFSSSDDDSQQRFALGFLFALIALVVSTILGVVVFKRGIAHAP
                     KAPAAAVVNAGGAAGAGVVIEDVASVVVEGGVVKFYFATGKADLAIGANEALAEVVKG
                     VIEGGKMAVVSGFHDATGDAALNAELAKQRALGVRDALKVLGVAEDKVELKKPEVTQG
                     SGSNAEARRVEVVLAP"
     gene            1111..1704
                     /locus_tag="PK518_06945"
     CDS             1111..1704
                     /locus_tag="PK518_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRM48689.1"
                     /translation="MPPLPAPLRDPAPMLERALIEWGGRCDLWVFGYGSLIWRPDFDF
                     VERRAAKVHGWHRALKMWSRLNRGTPECPGLVFGMLPGGSCRGMAFRVQQTHARQVMV
                     NLWQREMVLGVYDPRWLDCQTSDGMVRALAFTLPRSSPSYTGILADDEYRRIFAQASG
                     IYGSTRDYAEATHTELLRLGIYDRALARIVALSKSHD"
     gene            1725..2378
                     /gene="pdxH"
                     /locus_tag="PK518_06950"
     CDS             1725..2378
                     /gene="pdxH"
                     /locus_tag="PK518_06950"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRM48690.1"
                     /translation="MSKSTLSSSIADLRKSYERAELSEDASLADPLQQFDQWLHEAVN
                     AQVNEPNAMTVATVSSDLRPSTRIVLIKGYDAQGIVWYTNYDSRKGRQLAGNPYAALQ
                     FHWVELERVVRIEGIVERVSDEESDQYFHSRPLDSRIGAWASPQSQVIAGRSVLVTNA
                     ARYGAQFLLKPPRPPHWGGYRLKPDQWEFWQGRKSRLHDRLRYRLDGGAWVRERLAP"
     gene            complement(2385..3605)
                     /gene="chrA"
                     /locus_tag="PK518_06955"
     CDS             complement(2385..3605)
                     /gene="chrA"
                     /locus_tag="PK518_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRM48691.1"
                     /translation="MQPASLPQSHAPAANAWQVFLIFLRLGLTSFGGPVAHLAYFRTE
                     FVARRRWLSESDYADLVALCQLLPGPASSQVGMALGLRQGGFAGALTAWLGFALPSAL
                     VMALLGLGLIATPGLVPAGVLHGLKVAAVAVVAQAVWRMGGSLCRGPARLLLMGLTCA
                     LTLAIPGMAGQLGAMMIAALAGLGLWGRAGVAPEQPAHEAPGPGPWAVRGATLLILFA
                     AMLVLLPLAARAWPQAWLLLADAFFRSGALVFGGGHVVLPLLQAEVVSTGWVAQDDFL
                     AGYGLAQAMPGPLFTFAAFLGAAAGHGAGGWLWAAVCLLAIFLPSFLLVMGVSPFWES
                     LRRHAPLRAALSGVNAAVVGLLLASLYNPVWTSAIQGPADIFLALAVFAELMWGRMPV
                     WLVVPLCGVMGLCL"
     gene            complement(3671..4219)
                     /gene="msrA"
                     /locus_tag="PK518_06960"
     CDS             complement(3671..4219)
                     /gene="msrA"
                     /locus_tag="PK518_06960"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRM48692.1"
                     /translation="MSSILQSITLAGGCFWCTEAVFKRVRGVTEVQSGYANGDVADPS
                     YEQVCSGHTGHAEAVRVTFDPAEIGLRDILQIFFATHDPTTPNRQGNDVGTQYRSGIY
                     YSDPTHEQVAAVLLHQLQQEQAFGAPIVTELQPLVNFWPAESYHQDFYERNPNQGYCN
                     FVVAPKVSKFRKTFARHLKPDA"
     gene            4336..4998
                     /locus_tag="PK518_06965"
     CDS             4336..4998
                     /locus_tag="PK518_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM48693.1"
                     /translation="MSAALPCIPTSAIPKRGRRKDARPGELLDAALDLFVDKGYAATR
                     VEEVAARAGVSKGTLFLYFPSKQELFKAVVRQNIAGRFAEWDLELESYVGTSQDLVRY
                     CFETWWERIGSTKASGLGKLMLSEANNFPEIAEFYRHEVVEPGHQLIRRVLQRGMDRG
                     ELRALDLEHAVYLVLAPLMFLMFSQSTPALCIPNAERFSPQEYIRLQADNVLQGLCVR
                     SA"
     gene            5045..5755
                     /locus_tag="PK518_06970"
     CDS             5045..5755
                     /locus_tag="PK518_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRM48694.1"
                     /translation="MNLPLNTSIDIHDPVEQARYNMVEQQIRPWNVLDAGVLELLSRV
                     RREDFVPPSYQGLAFIDTEIPLNPDAQEAARLGQVMLAPRVDARMLQDLQIQPTDRVL
                     EIGAGSGYMAALLAACAERVVTLEIDPDLAEFARENLRSAGVDNADVRQADGARDPIP
                     DGPFDVIVLSGSVAEVPQKLLALLRDGGRLGAYVGQEPMMRATIVRRQGERFETSQPW
                     DVVVPRLRHFPEPSSFKF"
     gene            5779..6117
                     /locus_tag="PK518_06975"
     CDS             5779..6117
                     /locus_tag="PK518_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM48695.1"
                     /translation="MIDHVRPAQLADWLLRHEAGHPLVLDVREPWELQTASVAAQGFE
                     LVAIPMGQLPMRLTELDPNRPTACLCHHGMRSLRVAAFLMQNGFDQVANITGGIDAWS
                     HERDSAVPHY"
     gene            6214..7530
                     /locus_tag="PK518_06980"
     CDS             6214..7530
                     /locus_tag="PK518_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRM48696.1"
                     /translation="MSAVYALCVAAAPPAQAQSLSALIEHARGYDAAWQAARAQLQAA
                     GSRAEQARAGLLPSAALTGGANYSRTDMSRARLSDVNVNGPSQTLGVQATQPLYRPAN
                     RIAFEQGQRGTDFAQAQLEGAEQDLLIRVTQAYFDVLAAGDTLTFVRAQKTAVAEQLA
                     AAKRNFQVGTTTVTDEREAQARHDLVTAQEIAASNDLHVKRLALDQLVGRTGATPLPL
                     AQSAQLPAVQPDDVSNWVRSAEERQPQVRQALIALDMAKLETAKAKTGHLPTVDLQAG
                     YNVQRTPNGSISMPGVHSRTDAATVGVALNLPLFAGFAVQNRIKETLSLEDKARADLD
                     NARRNVAQAARAAFFGVQSGLSQVAALRAAESSSQSALDANKLGYQVGVRINIDVLNA
                     QSQLYQTKRDLAAARYNVLVGTLRLKQVAGTLTMDDVTAVDALLMR"
     gene            complement(7595..10300)
                     /locus_tag="PK518_06985"
     CDS             complement(7595..10300)
                     /locus_tag="PK518_06985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM48697.1"
                     /translation="MMEKHYLTSLFTPGSVIVLAGDPDAPESQTPQARALLDGLKAQK
                     FTGTLQFFDINTSGTLADLAQIRADLAIIVQPNKDLPAALEIAGRMNCRAALVLSNGV
                     DAELAGQLHKIARREGMHLLGPNSLGIQRPSLQLNASAAGPLAQEGSLALVCQSGALT
                     SSILDWASNNGVGFSSVISLGPHTDMGLSEALDFLANDARTQSIVVYMEGIQDARRFV
                     SAMRSTSHAKPVVVLKAGRKPAGNKAAQTHSGAIVGSDDVFDAVLRRAGAVRVRSFVA
                     LFSAAKCLASRYRPVGKRLAIVTNGGGPGVLAADWENEIGLDLGLLSPESSASLAPQL
                     PALASLGGLIDLSEDATPQHYARALQAAFSDRQIDGVLAIFSPKPGADAEGVAQALAD
                     AKRLAPKPLLSCWMGDATVVPARKILRSAQIPTFRTPEAAVGAFGNIASFYQNQQLLQ
                     QTPPSLTTLAKPDIDGARLVIESVLAERRNVLTEMESKTLLAAFHIPVTKTLLARNAH
                     EAMMIATQLGFPVALKIHSRDIAHKSDVGGVVLNLQSGAAVRDAYNDMVQRVARLQPD
                     ARIDGVTVQQMARARRGREICIGLVSDDPFGPVITFGAGGTMIELINDRAMELPPLNQ
                     FLARSLIGRARVAETLGEWRGASAVDMQALEQVLLRVSEMVCALPQLREMDINPLIVD
                     EQGAVAVDARIALHESAHASGRPESAGAGHYNHLSIHPYPARYEQIWPLRGGGECLVR
                     PIRPDDAQMVQRLVKELSPESRYFRFVSQIAELPPSMLSRFTLIDYDREMALVAVQRE
                     RIVDEEGEVSHKDRIVGVSRYVTNPDHSSCEFALLVADDFAGKGLGSRLMHSIMEVAR
                     DRGLAEIQGLVLANNPTMLKLMRRLGFEVRAFEEDPEFKLVVHQL"
     gene            complement(10441..11763)
                     /locus_tag="PK518_06990"
     CDS             complement(10441..11763)
                     /locus_tag="PK518_06990"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRM48698.1"
                     /translation="MQTIALWLYSLALWLAQPLLRRKLRRRAVNEPGYAVAVPERFGR
                     YAQPVAAPAPGGPLVWIHAVSLGETRAAAILLKELRIELPGMRLLLTHGTATGRAEGE
                     KLLQPGDLQVWQPWDTRGAVRRFLRQFRPAIGILMETEIWPNLVAGCRRERVPLVLAN
                     ARLNEKSRAGAQRLAWLSRPAYAALSAVWAQTEEDAARLRSVGAPVTGVFGNLKFDVE
                     PSATQLAQGRGWRQASLHPVVLLASSREGEEAMWLDVLKQKQALAPTGQAPTAINNEA
                     SSPVQWLVVPRHPQRFDEVQQLLEAAGLRVSRRSQWSEAPQNADVWLGDSLGEMALYY
                     GMAHVALLGGSFAPLGGQNLIEAAACGCPVVMGPHTFNFAQAADLAMAAGAAQRVSDM
                     AAGIAAACALAADGPRQAQLAQRCQSFTQTHRGAAQATARAVVRLLAA"
     gene            complement(11790..13178)
                     /locus_tag="PK518_06995"
     CDS             complement(11790..13178)
                     /locus_tag="PK518_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRM48699.1"
                     /translation="MQLSPAIFKAYDIRGIVPASLDEEVARALGRAFGTAALQQGERV
                     VAVGRDGRLSGPALSGALIQGLVDVGIEVIDVGLCTTPMLYFAASTLCASGIQVTGSH
                     NPKDYNGFKMVLAGRAIHGEEIQQLRRSMEAASWQPQDGGSVRQADVLPAYTERIVGD
                     VKLARPMKIVVDSGNGIAGASAPGIFRALGCEVVELFSEVDGNFPNHHPDPSKPENLR
                     DVIAALQATDAELGLAFDGDGDRLGIVTKDGQNIFPDRQMMLFAQDVLSRVPGAPILF
                     DVKCTQRLAPAISAAGGEPVMYKTGHSLIKARMKELAAPLGGEMSGHIFFKERWYGFD
                     DGTYAGCRLLEILSRSADPSSVLNALPTSHSTPELNVACAEGEPHRLTAELQALAADA
                     FAAPAAISTIDGLRVDWPDGFGLIRASNTTPVLVLRFEGHTDAALQRIEGEMLALLAR
                     VKPGAQVGGAAH"
     gene            13300..14805
                     /locus_tag="PK518_07000"
     CDS             13300..14805
                     /locus_tag="PK518_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HRM48700.1"
                     /translation="MSTAPHSPAPPAAAADEDEARYLALKARDARFDGRFFTGVTSTG
                     IYCRPVCAVRTPRRENCRFFALAAQAESAGFRPCLRCRPELAPQSAPWTLQDAGAILL
                     RQALRLLDAPEAWAEPEGGALVARLAARLGISDRHLRRIFEAALGISPLQYLQTRRLL
                     TAKQLLTDTRLPVTQVALASGFASLRRFNAAFGAHYGLNPTQLRREGTAQQNGGHPVH
                     LGYRPPLDVGALLDFFAARRIHGLEEVEGSTLRRTLRLAGGAGVHTGWLEARFDDTRP
                     QLLLRVSDGLLPALPQVIARVRALLDLDADPAAINAVLHPFFPSGDGLRVPGTLDGFE
                     LAVRAVLGQQITVAAARTLAERLVRQFGAPVDTPWPGLDRLFPTPAVLAAADGDALGQ
                     LGIVRQRQAAIVALARAVDGGQLDLSASADVEATLAALRQLPGIGDWTAQYIALRALR
                     WPDAFPAGDVALHKALGVRDQAQPARAAVDAAQAWRPWRGYGVIRAWSSLV"
     gene            14883..15428
                     /locus_tag="PK518_07005"
     CDS             14883..15428
                     /locus_tag="PK518_07005"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRM48701.1"
                     /translation="MQFPSDTVQSRIRTDLGVVSLAAGTRGLVGLWFDGQRHLPAQLD
                     GPGAWSGDDTHPVLRAAARQLAQYLAGTRQAFDLPLDLSGGTPFQQAVWRALLAIPSG
                     ATTSYGALARALGSPTAVRAVGAAVGRNPISIVVPCHRVLGAGGALTGYAGGLARKQA
                     LLRLEGAPPSIFPSQPLPAFA"
     gene            15425..16357
                     /locus_tag="PK518_07010"
     CDS             15425..16357
                     /locus_tag="PK518_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM48702.1"
                     /translation="MSSTPLSAGAAVPHHAWLGEFILLASLWGASFLFMRLGAAEFGP
                     LPTAGLRVLLATLFLWPILLHRGEWPQLRRHWRPVLLCGLINSAIPFALFAWAVMHIA
                     TGLASILNAAVPLFGALVAWLWLGDRINRLRWLGLALGLVGVALLAWRAPAGVGLKSG
                     QAGWAIGACLLASTCYAVAASFARRYLQGIPPLATATGSQLGAALGLALPMWWAWPAQ
                     TPGLRAWAAVAGLAVLCTGIAFILYFRIINHAGPSRALAVTFLAPVFAVLYGALFLGE
                     TVTPWMLGCGVVIVCGTMLSTGLVTGLWRTKAGA"
     gene            16386..16814
                     /locus_tag="PK518_07015"
     CDS             16386..16814
                     /locus_tag="PK518_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HRM48703.1"
                     /translation="MPSDPFDLQRFIAAQEQAYAPALAELRAGRKRTHWVWYIFPQLQ
                     GLGFSAMAQAYGMAGLDEARAYLEHPLLGARLVECVQAMLAHTGLPAAGILGEIDAIK
                     FRSCLTLFVAAGGQPVFHEALRRFFAGVPDAQTLALLQQN"
     gene            complement(16891..>18056)
                     /locus_tag="PK518_07020"
     CDS             complement(16891..>18056)
                     /locus_tag="PK518_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM48704.1"
                     /translation="TTGKPKGVQRDTGGYTVALAASMKHIFDAQAGQTYFATSDIGWV
                     VGHSYIIYGPLLAGMTTIMYEGLPTRPDAGVWWSIVEKYQVTHMFSAPTAVRVLKKQD
                     PEYLSKYNIKSLKALWLAGEPLDEPTATWISGALNVPIIDNYWQTETGWPILTLCNGV
                     QQQSPRFGSPGKAVYGYNVKLIDEATGQELTTPNQKGVVAIEGPLPPGCLQTVWRDDE
                     RFVSTYWNSIPGRQVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEECIAG
                     HPNIAEVAVVGVADQLKGQVAMAFAVPRDASGLVDDAARLKFEGEVMKQVDSQLGAVA
                     RPARVIFVNLLPKTRSGKLLRRALQAVAERRDTGDLTTMEDPVALQQIKDLMG"
BASE COUNT         2945 a         6197 c         5974 g         2940 t
ORIGIN      
        1 atatacatcg tatattattt ggtgtgataa gatcgccgca tgagaatcct ccacaccatg
       61 ctgcgcgtcg gcaacctcca gcgctcgatc gacttctaca cccaggtact gggcatgcag
      121 ttgctgcgca tgtccgagaa ccctgagtac aaatactcgc tggccttcct gggattcgat
      181 ggcggcaacc ccgaccaggc cgagatcgag ctgacctaca actggggcgt cgaaagctac
      241 gacatgggga cggcctacgg ccacatcgcg ctgggcgtac ctgatgccta tgcggcctgc
      301 gaaaagatca aggctgctgg cgggaatgtg actcgcgagg ccgggcctgt catgggcggc
      361 accacggtga ttgccttcgt caccgacccc gacggctaca agatcgaact catccagcgc
      421 gccagcgaag cggctggcgg tggcctgcgc taaaagtcac ggcgcatcag caaaaaagcc
      481 cggcaatgcc gggcttctcg ctcaagacag cgcggattca aggggccagt acgacttcga
      541 cgcggcgcgc ctcggcgttg ctgccgctgc cttgcgtgac ttcgggcttc ttcagctcca
      601 ccttgtcttc agccacgccc agaaccttga gggcgtcgcg cacgcccagg gcgcgctgct
      661 tggccagctc ggcgttgagt gcagcatcgc cggtcgcgtc atgaaaaccc gaaaccacgg
      721 ccatcttgcc accttcgatc acgcccttga ccacctcggc cagggcctcg ttggcgccaa
      781 tcgccaggtc ggccttgcca gtggcaaagt agaacttcac cacgccgcct tcgaccacca
      841 ccgaagccac atcctcaatc accacgccgg cacccgctgc accgcctgca ttgaccacgg
      901 ccgcggccgg tgccttgggg gcatgggcaa tgccacgctt gaagaccacc acacccaaaa
      961 tggtggatac caccagggcg atcaaggcga agaggaaacc gagtgcgaag cgttgctggc
     1021 tatcgtcgtc ggagctgctg aaggacatga tgaagactcc cgtgaataat gggttcgttg
     1081 gacaaaacct gctaattgta aagtctcgcc atgcctccgc ttcctgcacc cttgcgcgac
     1141 cccgccccaa tgctcgaacg cgcgctgatt gaatggggcg ggcgctgcga cctatgggtg
     1201 tttggctatg gctcgctgat ctggcgcccg gactttgact ttgtcgagcg ccgcgccgcc
     1261 aaggtgcatg gctggcaccg cgcgctgaag atgtggagcc gcttgaaccg cggcacgccc
     1321 gaatgtcccg gcctggtgtt cggcatgctg cccgggggca gttgccgcgg catggccttt
     1381 cgcgtgcaac agacccatgc gcgccaggtc atggtcaacc tgtggcagcg tgagatggtg
     1441 ctcggggtgt acgacccgcg ctggctcgac tgccagacat ccgatggcat ggtgcgcgcg
     1501 ctggccttca cgttgccgcg cagcagtccc agctatacgg gcatattggc cgacgacgag
     1561 taccgccgca tcttcgcgca ggccagcggc atctacggca gcacgcgtga ctatgcagag
     1621 gccacgcaca ccgaactgct gcgtttgggc atatacgatc gggcgctggc acgcatcgtt
     1681 gcgctctcca aatcgcacga ttgagcaagg gcacaatcga gcccatgagc aaaagcaccc
     1741 tctcctcttc catcgctgac ctgcgcaaga gctacgagcg cgccgaactc agcgaagacg
     1801 cctcccttgc cgacccgctg cagcagttcg accaatggct gcacgaggca gtcaacgcgc
     1861 aggtgaacga gcccaacgcc atgaccgtgg ccaccgtcag cagcgacctg cgcccgagta
     1921 cgcgcatcgt gctcatcaag ggctatgacg cgcaaggcat cgtctggtac accaattacg
     1981 acagccgcaa gggccgccag ctggcgggca acccctatgc ggcgctgcaa ttccactggg
     2041 tggaactgga gcgcgtggtg cgcatcgagg gcattgtcga gcgcgtgagc gacgaggaaa
     2101 gtgatcagta tttccacagc cgcccgctgg attcgcgcat tggcgcctgg gccagcccac
     2161 aaagtcaggt gattgccggc cgcagcgtgt tggtgacaaa tgcagccagg tacggcgcgc
     2221 agtttctgct caaaccccct cggccgccgc attggggcgg ctaccgcctg aagcccgatc
     2281 agtgggagtt ctggcagggc cgaaaaagcc gcctgcacga ccggctgcgc taccggcttg
     2341 acggcggtgc ttgggtgcgc gagcgcctgg cgccctgacc ccggctacaa acacaacccc
     2401 atcacgccac acaggggcac caccagccag actggcatgc gcccccacat gagctcggca
     2461 aacaccgcca gcgccaggaa aatatccgct ggaccctgga tggcactcgt ccagacgggg
     2521 ttgtagagcg aggccagcag caggccgacc accgccgcat tcacgcccga cagcgccgcc
     2581 cgcagcggcg cgtgccggcg cagcgattcc cagaatggag acacccccat aaccagcaaa
     2641 aaagatggca ggaatatggc caacaagcac actgccgccc acagccagcc acccgcgcca
     2701 tggcctgccg ctgcacccag gaacgcggcg aatgtgaaca acggcccggg catggcctgc
     2761 gccaggccgt agccagccag aaagtcgtcc tgcgccaccc agccggtgct gaccacctcg
     2821 gcctgcagca gcggcagcac cacatgacca ccgccaaaca ccagggcgcc ggaacgaaag
     2881 aaggcgtcgg cgagcagcag ccatgcctgt ggccaggcgc gtgcggccag cggcagcaat
     2941 accagcatgg cggcaaacag aatcaacagg gtagcccccc tgactgccca cgggccaggg
     3001 ccaggcgctt catgggcagg ctgctcaggc gcaacgcccg ccctgcccca caggcccagg
     3061 ccggccagtg ccgcaatcat catggcgccc agttgccctg ccatgccagg tatcgccagg
     3121 gtcagggcgc aggtcaaacc catcaacaac aaccgggctg gtccacggca caggctgcca
     3181 cccatgcgcc acacggcttg cgccaccacc gctacggcgg ccaccttcag accatgcagc
     3241 acgcctgcgg gcaccagtcc gggggttgca atgaggccca ggcccagcag tgccatgacc
     3301 aatgcagagg gcaaggcaaa gcccagccag gctgtcaagg ccccggcgaa gccgccctga
     3361 cgcaggccca gcgccatgcc cacctggctg ctcgccgggc cgggcaaaag ctggcacagg
     3421 gccaccagat cggcatagtc gctttcactc agccagcgcc ggcgcgccac gaactccgta
     3481 cggaaatagg ccaggtgcgc cacaggcccg ccaaatgatg tcaggcccag gcgcagaaag
     3541 atcagaaaca cctgccaggc gttggctgca ggggcatggc tttgaggcag ggaggcgggt
     3601 tgcatgcgga gggcatttcg aagcagcagg cgagctgccg gctgaaagcc ggcgacgata
     3661 ctgcaaaatt tcaggcgtct ggcttgagat ggcgtgcaaa cgtcttgcgg aacttgctca
     3721 ccttgggcgc cacgacaaag ttgcagtaac cctgattcgg attgcgctca tagaagtcct
     3781 ggtgatagct ctcggccggc cagaagttca ccaatggctg cagctcggtg acgatggggg
     3841 cgccgaaagc ctgctcctgc tgcaactggt gcagcagcac cgcggcaacc tgctcgtgcg
     3901 tggggtcgct gtagtagatg ccgctgcggt actgcgtgcc cacatcattg ccctggcggt
     3961 ttggcgtggt ggggtcgtgg gtggcaaaaa agatctgcag tatgtcgcgc aagccgatct
     4021 cggccgggtc aaacgtgacg cgcaccgcct cggcatggcc cgtgtgacca ctgcacacct
     4081 gctcgtagct tggatctgcc acatcgccat tggcatagcc gctttgcacc tcggtcacgc
     4141 cgcgcacacg cttgaataca gcctcggtgc accagaaaca accgccagcc agggtgatgc
     4201 tttgcagaat tgacgacata tggtgctcct gaaaatcgca tcatagaaac accgccatat
     4261 gcttgatgat caggatgcac gttgacgcaa acaacggagg gttgttacat tactaacccg
     4321 aaagtcagta actccatgag cgccgccctg ccctgcatcc ccacatcagc aatccccaag
     4381 cgaggccgtc gcaaggacgc caggccgggt gagctgctgg atgccgcgct agatttgttc
     4441 gtggacaagg gctacgccgc cacccgggtc gaagaagtgg cggcgcgcgc cggtgtctcc
     4501 aagggcacgc tgtttctgta cttcccgagc aaacaagagc tgttcaaggc cgtggtgcgc
     4561 cagaacattg ccgggcgctt tgccgaatgg gatctggagc ttgaatccta tgtgggcaca
     4621 agccaggatc tggtgcggta ctgctttgaa acctggtggg aacgcatagg ctccaccaag
     4681 gcctcgggcc tgggcaagct catgctcagc gaggcgaaca atttccccga aatcgccgag
     4741 ttctaccgcc acgaggtggt cgagcctggc caccagttga tccgccgtgt gttgcagcgt
     4801 ggcatggatc gcggtgagct gcgcgcgctg gatctggagc acgccgtgta cctggtgctg
     4861 gcgccgctca tgtttctcat gttctcgcag tcgacgccgg cgttgtgcat tcccaacgcc
     4921 gagcgttttt caccacagga atacatacgc ctgcaggccg acaatgtttt gcagggtctg
     4981 tgcgtgcgct cggcctaaaa tcgcaggttt atccgatcac agtgaccgcc ttccggagta
     5041 ctccatgaac ttgcctctga acacgtccat cgacattcac gaccctgtcg agcaggcccg
     5101 ctacaacatg gtggagcagc agatccggcc gtggaacgtg ctcgatgccg gggtgctcga
     5161 gctgctgtca cgcgtgcgcc gcgaagactt tgtgccgccc agctaccagg gcctggcctt
     5221 catcgacacc gaaatcccgc tcaaccccga cgcgcaagaa gccgcacgtc tgggacaggt
     5281 catgctcgcg ccgagggtgg acgcgcgcat gctgcaggat ctgcaaatcc agcccaccga
     5341 ccgcgtgctg gagataggcg ccggctcggg ctacatggcc gccctgctcg ccgcctgcgc
     5401 cgagcgtgtc gtaacgctgg aaatcgaccc tgacctggcc gagtttgcgc gcgagaacct
     5461 gcgtagcgcc ggtgtggaca acgccgacgt gcgccaggcc gacggcgcac gcgaccccat
     5521 tcccgacggc ccgttcgacg tcatcgtgct cagcggctcg gtggccgagg tgccgcaaaa
     5581 gctgctggcg ctgctgcgcg acggtggccg cctgggcgct tatgtggggc aagagccgat
     5641 gatgcgtgcc accatcgtgc gccgccaggg cgagcgcttc gagaccagcc agccctggga
     5701 cgtggtcgtg ccgcgcctgc gccacttccc cgagccctct tccttcaagt tctgaacgct
     5761 gcacccaacc ggccttgcat gattgaccat gtccgtcccg cccagctggc cgattggctc
     5821 ctcaggcatg aggccggaca tccgttggtg ctggacgtgc gcgagccctg ggagctgcag
     5881 accgccagcg tggcggccca gggcttcgaa ctcgtggcca tccccatggg tcagctgccg
     5941 atgcggctga ccgaacttga ccccaaccgc cccaccgcct gcctgtgcca ccacggcatg
     6001 cgcagcctgc gggtggctgc ctttctgatg caaaacggct ttgaccaggt ggccaacatc
     6061 accgggggca tagacgcctg gtcgcatgag cgcgattcgg ccgtgccgca ttactgactt
     6121 gcggcccctc acaggccgca cccccaatct gccccagcca tccatttcaa cgccatgaaa
     6181 gcattgcagc gggttttctc ctccaccctt cgcatgagtg ccgtctacgc cctttgcgtt
     6241 gccgcggcac cccccgcgca ggcgcaaagc ctgtcggcat tgatcgagca tgctcgcggc
     6301 tacgacgcgg catggcaggc cgcacgggcg caactgcagg ccgcgggcag ccgtgccgaa
     6361 caggcccgtg ccggcctgct gcccagtgct gcgctgaccg gtggcgcgaa ttattcgcgc
     6421 accgacatgt cacgcgctcg tctgtccgac gtgaatgtca atggcccgtc gcaaacgctg
     6481 ggtgtgcagg cgacgcagcc cctgtaccgc ccggccaacc gcatcgcctt tgagcagggc
     6541 cagcgcggca cggactttgc ccaggcgcag ctcgaaggcg ccgagcagga tctgctgatt
     6601 cgcgtcaccc aagcctactt tgacgtgctg gccgcaggcg acacgctgac cttcgtgcgc
     6661 gcacaaaaga ccgctgtggc ggaacagctt gccgcggcca agcgcaactt ccaggtcggc
     6721 accaccaccg tcaccgacga gcgcgaggcg caggcccggc atgacctggt caccgcccag
     6781 gaaatcgccg ccagcaatga cctgcatgtg aagcgcctgg cactcgacca gctggttggg
     6841 cgcaccggcg ccacccccct gcccctggct cagtccgcgc agttgccggc cgtgcagccc
     6901 gatgacgtca gcaactgggt gcgcagcgcc gaggagcgcc agccccaggt acgccaggcc
     6961 ctgattgcgc tggacatggc caagctggaa accgccaagg ccaaaaccgg ccacctgccc
     7021 accgtcgacc tgcaggcagg ctacaacgta caacgcacac ccaacggcag catcagcatg
     7081 ccgggcgtgc attcgcgcac cgacgccgcc accgtgggcg tggccctgaa cctgccgctg
     7141 tttgccggct ttgccgtgca aaaccgcatc aaggaaaccc tgtcactcga agacaaggcc
     7201 cgcgccgacc tggacaacgc gcgccgcaac gtagcccagg ccgcgcgtgc cgctttcttc
     7261 ggcgtgcaat cgggcctgag ccaggttgcc gcgctgcgcg ctgccgagtc ctccagccaa
     7321 agcgcgctgg acgccaacaa gctcggctac caggtaggag tgcgcatcaa catcgacgtg
     7381 ctcaacgccc aaagccagct ctaccagacc aagcgcgacc tggccgccgc gcgctacaac
     7441 gtactggtcg gcacgctgcg cctgaagcag gtggcgggca cgctgacgat ggatgacgta
     7501 acggcggtgg atgcgttgct gatgcgatag ttttcagcac tctgcgctta ccagacaagc
     7561 gcaaagtgct atattttcaa aagcaaccga ggacttacag ctggtgcacc accagcttga
     7621 attccggatc ctcctcaaag gcccgcacct cgaagcccag tctgcgcatg agtttgagca
     7681 tggtggggtt gttggccagc accaggccct ggatctcggc caggccgcgg tcgcgggcga
     7741 cttccatgat gctgtgcatc aggcgcgagc ccagaccctt gccggcgaag tcgtcggcca
     7801 ccagcagggc aaactcacag ctgctgtgat ccgggttggt gacatagcgt gacacgccga
     7861 cgatgcgatc cttgtggctg acctcgccct cttcgtccac gatgcgttcg cgctgcacgg
     7921 ccaccagggc catttcgcgg tcgtagtcga tcagggtgaa gcgcgacagc atggacggcg
     7981 gcagctcggc gatctgcgag acaaagcgga agtagcggct ctcgggcgac agctccttga
     8041 ccaggcgctg caccatttgc gcgtcgtccg ggcggatcgg gcgcaccagg cattcgccac
     8101 cgccgcgcag cggccagatt tgctcgtagc gcgccgggta ggggtgtatc gacaggtggt
     8161 tgtaatggcc ggcgccggcg ctttcggggc gaccgctggc gtgggccgat tcatgcaggg
     8221 cgatgcgcgc atccacggcc acggcgccct gctcgtccac gatcagcggg ttgatgtcca
     8281 tctcgcgcag ttggggcagc gcgcagacca tttctgagac gcgcagcagc acctgctcca
     8341 gcgcctgcat gtccacggcg gaggcaccac gccattcgcc cagggtttcg gccacgcgtg
     8401 cacggccgat caggctgcgc gccagaaact ggttcaacgg cggcagctcc atggcgcggt
     8461 cgttgatgag ctcgatcatg gtgccgcctg cgccaaaggt gattaccggg ccaaacgggt
     8521 cgtcgctcac caggccgatg cagatctcgc gcccacggcg cgcacgcgcc atttgctgca
     8581 cggtgacgcc atcgatgcgc gcatcgggct gcaggcgcgc cacccgctgc accatgtcgt
     8641 tgtaggcgtc gcgcacggcg gcgccgcttt gcaggttgag taccacgccg cccacgtcgg
     8701 atttatgcgc gatgtcgcgc gagtggatct tcagcgccac cgggaagccc agttgcgtgg
     8761 cgatcatcat ggcctcgtgg gcattgcgtg ccagcagcgt cttggtgacc gggatgtgga
     8821 aggcggccag cagcgtcttg gactccatct cggtgagcac gttgcggcgc tcggccagca
     8881 cgctttcgat gaccaggcgc gcgccatcga tgtcgggctt ggccagcgtg gtcagtgacg
     8941 gaggcgtttg ctgcagcagc tgctggtttt ggtagaacga ggcgatgttg ccaaaggcgc
     9001 ccacggccgc ctcgggcgtg cggaaggtgg ggatttgcgc gctgcgcaga atcttgcgcg
     9061 ccggcaccac ggtggcgtcg cccatccagc agctgagcag cggcttgggt gccagccgct
     9121 tggcgtcggc cagggcctgg gccacgccct cggcgtcggc gccgggtttg ggcgagaaga
     9181 tggctaacac gccgtcgatc tgccgatccg agaacgcggc ctgcagcgcc cgggcgtaat
     9241 gctggggcgt ggcgtcctcg gacaggtcga tgagcccgcc gagcgaggcc agggccggca
     9301 gctgcggcgc caggctggcg ctggattctg gcgagagcag gcccaggtcg aggccgattt
     9361 cgttctccca gtcggcggcc agcacgccgg ggccgccgcc gttggtgacg atggccaggc
     9421 gcttgcccac gggccggtag cgcgatgcca ggcatttggc ggccgaaaac aacgccacga
     9481 aggagcgcac gcgcaccgcg ccggcgcggc gcagcacggc gtcaaagaca tcgtcgctgc
     9541 cgacgatggc accgctgtgc gtctgcgcgg ccttgttgcc cgccggcttg cgcccggctt
     9601 tcagcaccac caccggcttg gcatgcgaag tcgagcgcat ggcgctcaca aagcggcgtg
     9661 cgtcctgtat gccttccatg tagaccacga tgctttgcgt gcgcgcgtcg ttggccagaa
     9721 agtccagcgc ctcgctcagg cccatgtcgg tgtgcgggcc cagcgagatc acgctggaaa
     9781 agcccacgcc gttgttgctg gcccagtcca ggatggacga ggtgagcgcg cccgactgac
     9841 acaccagtgc cagagatccc tcctgcgcca gcggtcccgc ggcgctggcg ttgagctgca
     9901 gcgatgggcg ctgtatgccc agcgagttgg ggcccagcag gtgcatgccc tcgcgccggg
     9961 caatcttgtg cagctgccct gccagctcgg cgtccacgcc attggacagc accagggcag
    10021 cgcggcagtt catgcggccg gcgatctcca gcgccgccgg caagtccttg ttgggctgga
    10081 cgatgatggc caggtcggcg cgaatctgcg ccagatcggc cagggtgcca ctggtgttga
    10141 tgtcaaaaaa ctgcaacgtg ccggtgaatt tctgcgcctt cagcccgtcg agcaatgcgc
    10201 gcgcctgggg agtttgtgac tccggggcgt cggggtcacc agccagcacg atgaccgagc
    10261 cgggggtgaa cagggaagtg aggtagtgct tttccatcat gatggttgcg attttctacg
    10321 gtctagggat aacccgtaaa tacgtacgtg tccgtatgtg gaaattgttg cttgaatgta
    10381 accgtaacga tggtatgcaa tgctgcggac tgcaccgacg cggccggcag ggcaacaggc
    10441 tcacgccgcc agcaaccgca ccaccgcccg agccgtggcc tgggcggcgc cacggtgggt
    10501 ctgggtaaag ctctggcagc gctgggccag ctgtgcctgg cgcgggccat ccgcggccag
    10561 ggcgcaggcg gcggcaatgc cggcggccat gtcgctcacg cgctgggcgg cgccggcggc
    10621 catggccaga tcggccgcct gggcaaagtt gaaggtgtgc gggcccatga ccacggggca
    10681 gccgcaggcc gccgcctcga tcaggttctg cccgcccagc ggcgcaaagc tgccgcccag
    10741 cagcgccaca tgggccatgc cgtagtacag cgccatctcg cccagcgagt cgcccagcca
    10801 gacatcggcg ttctgtggcg cctcgctcca ttggctgcgc cgcgacacgc gcaggccggc
    10861 ggcctccagc agttgctgta cctcatcaaa gcgctggggg tggcggggta cgaccagcca
    10921 ctggacgggt gatgatgctt cgttatttat agctgttggc gcttgtcctg tgggcgctag
    10981 agcctgtttt tgctttaaaa catccagcca catcgcctcc tcgccctcgc gcgagctggc
    11041 cagcagcacc acggggtgca gactggcctg gcgccagccg cgtccctgcg ccagctgcgt
    11101 ggccgagggc tcaacatcaa atttgaggtt gccgaatacc ccggtaacgg gcgcgcccac
    11161 gctgcgcagg cgcgccgcgt cctcctcggt ctgggcccag accgccgaca gcgcggcata
    11221 ggccgggcgc gacagccagg ccaggcgctg cgcgccggcg cgtgattttt cattcaggcg
    11281 cgcattcgcc agcaccagcg gcacccgctc gcgccggcag ccggccacca ggttgggcca
    11341 gatctccgtc tccatcagga tgccgatggc cgggcggaac tggcgcagaa agcgccgcac
    11401 ggcaccgcgc gtatcccagg gctgccagac ctgcaggtcg ccgggctgca gcagtttttc
    11461 gccctcggcc cggcccgtgg ccgtgccgtg cgtcagcagc aggcgcatgc cgggcagctc
    11521 tatgcgcaac tctttgagca ggatggcggc ggcgcgcgtc tcgcccaggg acacggcatg
    11581 tatccacacc agcggcccgc ccggcgccgg cgccgccacc ggctgcgcgt agcggccaaa
    11641 gcgctcgggc acggcgacgg cgtagccggg ctcgttcacg gcgcgccggc gcagcttgcg
    11701 tctgagcagc ggctgggcca gccacagggc caggctgtac agccacaggg cgatggtctg
    11761 catgggcgcc gcagtgggtg gcgccgggct cagtgggccg cgccgccgac ctgggcgcca
    11821 ggcttgacgc gcgccaagag cgccagcatc tcgccctcga tgcgctggag cgccgcatcg
    11881 gtgtggccct caaagcgcag caccagcacc ggcgtggtgt tgctggcgcg gatcaggcca
    11941 aagccgtcgg gccagtccac gcgcaggccg tctatggtgc tgatggcggc gggcgcggca
    12001 aaggcgtcgg ccgccagggc ctgcagctcg gccgtgaggc ggtgcggctc gccctcggcg
    12061 caggccacgt tcagctcggg cgtgctgtgg ctggtgggca aggcgttgag cacgctgctg
    12121 gggtcggcgc tgcggctcag gatctccagc aggcggcagc cggcgtaggt gccgtcgtca
    12181 aagccgtacc aacgctcctt gaagaagatg tggccgctca tctcgccgcc cagtggggcg
    12241 gccagctcct tcatgcgcgc cttgatcagc gagtggccgg tcttgtacat gacgggctcg
    12301 ccgcccgccg cactaatggc cggcgccagg cgttgggtgc atttcacgtc gaacagaatc
    12361 ggcgcgccgg gcacgcgcga cagcacgtcc tgggcgaaca gcatcatctg gcgatcgggg
    12421 aagatgttct ggccgtcctt ggtgacgatg ccgaggcggt cgccgtcgcc gtcaaaggcc
    12481 aggcccagct cggcgtcggt ggcttgcagg gcggcgatca cgtcgcgcag gttttcgggc
    12541 ttgctcgggt cggggtggtg gttgggaaag ttgccatcca cctcggagaa cagctccacc
    12601 acctcgcagc ccagggcgcg gaagatgccc ggcgccgagg cgccggcgat gccgttgccg
    12661 ctgtcgacca cgatcttcat gggccgcgcc agcttcacgt cgcccacgat gcgctctgtg
    12721 taggctggca gtacgtctgc ctggcgcacg ctgccgccgt cctgcggctg ccagctcgcg
    12781 gcctccatgc tgcggcgcag ctgctggatc tcctcgccat ggatggcgcg gccggccagc
    12841 accatcttga agccgttgta gtccttgggg ttgtggctgc ccgtgacctg gatgccgctc
    12901 gcgcacagcg tgctggcggc aaagtacagc atgggcgtgg tgcacaggcc cacgtcgatg
    12961 acctcgatgc ccacatccac caggccctgg atcaaggcac ccgagagcgc cggccccgac
    13021 agccgcccat cgcgccccac ggccacgacg cgctcgccct gctgcaatgc cgccgtgcca
    13081 aaggcccggc ccagggcgcg ggccacttcc tcatcgaggc tggcgggcac gatgccgcgg
    13141 atgtcgtagg ccttgaagat ggcgggggag agttgcacgg agtgacctgt cgaaagtgaa
    13201 caaagcctgg attgtaggtg cccgtcccgg ctagacagcg ccccaggcgc atgtccgaaa
    13261 acggcgagtg cgcgccccgg tgcggcctat catggcccca tgtccaccgc cccgcactcc
    13321 cctgccccgc cggccgccgc tgccgacgag gacgaggccc gctacctggc cctgaaggcc
    13381 cgcgacgcgc gctttgacgg gcgcttcttc accggcgtga cctccaccgg catctactgc
    13441 cgcccggtct gcgccgtgcg cacgccgcgg cgcgagaact gccgcttctt cgccctggcc
    13501 gcccaggccg agagcgccgg ttttcgcccc tgcctgcgct gccggcccga gctggcgccg
    13561 cagtccgcgc cctggacgct gcaggacgcg ggcgccatcc tgctgcgcca ggcgctgcgg
    13621 ctgctggatg cgcccgaggc ctgggccgag cccgagggcg gcgccctggt ggcacggctg
    13681 gcggcgcgct taggcatcag cgaccggcac ctgcgccgca tcttcgaggc cgctttaggc
    13741 atctcgccgc tgcagtacct gcagacgcgg cggctgctga cggccaagca gctcttgacc
    13801 gacacgcgcc tgcccgtgac ccaggtggcg ctggccagcg gctttgccag cctgcggcgc
    13861 ttcaatgcgg cgtttggcgc gcactacggc ctcaacccca cccagctgcg gcgcgagggc
    13921 acggcgcagc aaaacggcgg ccaccccgtg cacctgggct accggccgcc gctggacgtg
    13981 ggggcgctgc tcgacttttt tgcggcgcgg cgcattcacg gcctggagga ggtcgagggc
    14041 agcaccctgc gccgcacgct gcggctggcg ggcggcgccg gcgtgcatac gggctggctg
    14101 gaggcccgct ttgacgacac ccgcccccag ctgctgctgc gcgtgagcga cggcctgctg
    14161 cccgcgctgc cccaggtgat tgcccgcgtg cgcgccctgc tcgacctgga cgccgacccg
    14221 gcggccatca acgccgtgct gcaccccttc ttccccagcg gcgacggcct gcgcgtgccg
    14281 ggcacgctgg acggcttcga gctggcggta cgcgcggtgc tgggccagca gatcacggtg
    14341 gctgcggcac gcaccctggc cgagcgcctg gtgcggcagt ttggcgcgcc cgttgacaca
    14401 ccctggccgg ggctcgacag gctgtttccc acgcccgccg tattggctgc ggccgatggc
    14461 gatgcactcg gccaactggg catcgtgcgc cagcgccagg ccgccatcgt ggcgctggcg
    14521 cgtgccgtcg atggcggcca gctcgatctg tcggccagcg ccgatgtgga agccaccctg
    14581 gccgccctgc gccagctgcc cggcatcggc gactggacgg cccagtacat cgccctgcgc
    14641 gccctgcgct ggcccgacgc ctttccggcc ggcgacgtgg cgctgcacaa ggccctgggc
    14701 gtgcgggacc aggcccagcc ggcgcgcgcg gccgtcgatg ccgcccaggc ctggcgcccc
    14761 tggcgcggct atggcgtgat acgcgcctgg agcagcctgg tgtagtagct atcaaataaa
    14821 tagctgtttg cgcatgtccc acaagcgcca tcggcctttt tcgcttctaa tcgaggttca
    14881 tcatgcaatt cccctccgat accgtacaaa gccgcatccg caccgacctg ggtgtggtga
    14941 gcctggccgc cggcacgcgc ggcctggtcg gcctgtggtt tgacggccag cgccacctgc
    15001 ccgcgcaact cgatgggccc ggcgcctggt ccggcgacga cacccacccc gtgctgcgcg
    15061 cggcggcccg gcaactcgcg caatacctgg ccggcacgcg ccaggcgttc gacctgccgc
    15121 tggatctgtc cggcggcacg ccgttccagc aggccgtgtg gcgcgcgctg ctggccattc
    15181 caagcggcgc cacgaccagc tacggcgcac tggcgcgggc cctgggcagc ccgaccgccg
    15241 tgcgcgccgt gggcgcggcc gtggggcgca acccgatcag catcgtcgtg ccctgccacc
    15301 gcgtgctggg cgcgggcggt gcgctgaccg gctatgccgg cggcctggcg cgcaagcagg
    15361 cgctgctgcg gctggaaggg gcgccgccca gcatcttccc atcacaaccc ctgcccgcct
    15421 tcgcatgagc agcacacccc tgtccgccgg cgcggccgtg ccacaccacg cctggctggg
    15481 cgaattcatc ctgctggcca gcctgtgggg cgcttcgttc ctgttcatgc gcctgggcgc
    15541 ggccgagttc gggccgctgc ccacggccgg gctgcgcgtg ctgctggcca cgctgttcct
    15601 gtggcccata ttgctgcacc ggggcgaatg gccgcagctg cggcgccact ggcggccggt
    15661 gctgctgtgc gggttgatca actcggcgat tccattcgcg ctgtttgcct gggccgtgat
    15721 gcacatcgcc accggcctgg cctccatcct gaacgccgcc gtgccgctgt ttggcgccct
    15781 ggtggcctgg ctctggctgg gcgaccgcat taaccgcctg cgctggctgg gcctggcgct
    15841 gggcttggtg ggcgtggccc tgctggcctg gcgcgcgcct gcgggcgtgg ggctcaagag
    15901 cggccaggcg ggctgggcga ttggcgcctg cctgctggcc tccacctgct acgccgtggc
    15961 ggccagcttt gcgcggcgct acctgcaggg cattccgccg ctggccactg ccaccggcag
    16021 ccagctgggc gcggccctgg gcctggccct gcccatgtgg tgggcctggc cggcgcagac
    16081 acccggcctg cgcgcctggg ccgccgtcgc cgggctggcg gtgctgtgca cgggcattgc
    16141 cttcattttg tactttcgca tcatcaacca cgccgggccc agccgggccc tggccgtgac
    16201 ctttctggcg ccggtgttcg cggtgctgta cggcgcgctc ttcctgggcg agacggtcac
    16261 gccgtggatg ctgggctgcg gcgtggtgat cgtctgcggc accatgctgt ccaccggcct
    16321 ggtgactggc ctgtggcgca ccaaggctgg cgcgtagtcc gcctaccgga taaagggggt
    16381 atgccatgcc atccgatccc ttcgacctgc aacgcttcat cgccgcccag gaacaggcct
    16441 atgccccggc cctggccgag ctgcgggccg ggcgcaagcg cacgcattgg gtctggtaca
    16501 tctttccgca gctgcagggt ctgggtttca gtgccatggc gcaggcctat ggcatggccg
    16561 gcctggacga ggcgcgggcc tatcttgagc acccgctgct gggcgcccgg ctggtcgaat
    16621 gcgtgcaggc catgctggcg cacacgggcc taccggcggc cggcattctg ggcgagatcg
    16681 acgccatcaa gttccgctcc tgcctgacgc tgtttgtggc ggccggggga cagccggtgt
    16741 tccacgaggc gttgcggcgt ttctttgccg gcgtaccaga cgcccagacc ctggccttgc
    16801 tgcagcagaa ctgataggca agaaaaaagc ccctggcgcc catacaacgg gcggcaggag
    16861 ctataaaaac aagagtgaat tccggcccga tcagcccatc agatccttga tctgctgcag
    16921 cgccaccggg tcttccatgg tggtcaggtc gccggtgtcg cggcgctcgg ccacggcctg
    16981 cagggcgcgg cgcagcagct tgccgctgcg cgtcttgggc agcaggttga cgaagatcac
    17041 gcgcgccggg cgggccacgg cgcccagctg gctgtccacc tgcttcatga cctcaccctc
    17101 gaacttcagg cgcgcggcgt cgtccaccag gcccgaggca tcgcgcggca cggcaaaggc
    17161 catggccacc tggcccttga gctggtcggc cacgccgacc acggccacct cggcaatatt
    17221 cggatggccg gcgatgcatt cctcgatctc gcgcgtgccc aggcggtggc cggcgacgtt
    17281 gatcacgtcg tcggtgcggc ccaggatgaa gtagtagccg tcggcgtcgc ggatgcccca
    17341 gtcgaaggtg ctgtagacct ggcggccggg gatgctgttc cagtaggtgc tgacaaagcg
    17401 ctcgtcgtcg cgccacacgg tctgcaggca gccggggggc agcgggcctt cgatggccac
    17461 cacgcccttc tggttggggg tggtcagctc ctggcccgtg gcctcgtcga tgagcttgac
    17521 gttgtagcca tagaccgcct tgcccgggct gccaaagcgc ggcgactgct gctgcacgcc
    17581 gttgcacagc gtgaggatgg gccagccggt ctcggtctgc caatagttgt cgatgatggg
    17641 cacattcagc gcgccgctga tccacgttgc cgtgggctcg tccagcggct cgccggccag
    17701 ccacagggcc ttgaggctct tgatgttgta cttgctgagg tactcgggat cctgcttttt
    17761 cagcacgcgc acggccgtgg gggccgagaa catatgcgtg acctggtact tctcgacgat
    17821 gctccaccac acgcccgcat ccgggcgcgt gggcaggccc tcgtacatga tggtcgtcat
    17881 gcccgccagc agcgggccgt agatgatgta gctgtgaccc accacccagc cgatgtcgct
    17941 ggtggcaaag taggtctggc ccgcctgcgc atcaaaaata tgcttcatgc tggccgccag
    18001 cgccacggtg tagccgccgg tgtcgcgctg cacgcccttg ggcttgccgg tggtgc
//
LOCUS       DAOMTQ010000052        17603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_447_length_17603_cov_27.245726, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000052 DAOMTQ010000000
VERSION     DAOMTQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 17603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17603
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_17603_cov_27.245726"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1581)
                     /gene="nadB"
                     /locus_tag="PK518_07025"
     CDS             complement(1..1581)
                     /gene="nadB"
                     /locus_tag="PK518_07025"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRM48705.1"
                     /translation="MASHDFDVLIVGSGLAGLSAALQLAPTHRVAVITKRELKDGASN
                     WAQGGIAAVLADDDSFAAHIQDTLVAGAGLCDMATTRFVVENAPAAIGWLRALGVPFT
                     LEGSGLHLTREGGHSARRIVHATDATGAAVQKTLTEVVRATPGITVFEQHTLVDLITT
                     AKLGLAGQPRCVGLYALDQASDSVVTFRAPHTILATGGAGKVYLYTTNPDTATGDGIA
                     AAWRAGCRVGNMEFVQFHPTGLYHPHEKSFLISEAVRGEGGQLKLPDGTRFMPAHDAR
                     AELAPRDVVARAIDFEMKKHGLDCVHLDISHQSPEFLREHFPNILAHCAALGIDITRE
                     PIPVVPTAHFTCGGVLTDLSGRTDLAGLLAVGEVAYTGLHGANRLASNSLLECLVFAR
                     AACSYITQAQPAKSPALPPWDDSRVGDADESVVISHNWDELRRFMWDYVGIVRTNKRL
                     ERAAHRIALLKGEIHEFYAHFHVTRDLLELRNLVQVADLIVQSAQMRHESRGLHYSRD
                     WPDMAAPAAPTILVPPLR"
     gene            complement(1648..2517)
                     /locus_tag="PK518_07030"
     CDS             complement(1648..2517)
                     /locus_tag="PK518_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM48706.1"
                     /translation="MIQTDTAAAGVDRLSALLERFRVRAHLFHAGPLCGVTPFAAEPG
                     RAFLHVLRRGELEVTHRPRSGAPRRLRLNQPTLLLYPRPLEHDFYNAPQEGSDFVCAT
                     LDFDGGPHHPLVQALPAFLALPIAEVDGIEQTLTLLFAETERVRCGQRLLADRLFEVL
                     LLQLLRWLLDHPADGGMQPGLLAGLAHPKLARALTRLHEQPGANWSLATMAQAAGMSR
                     SAFAAEFRAALGTTPGAYLLAWRVSLAQGLLRRGLQVRQISDQLGYASPAAFTRAFAQ
                     AVGTAPREWLKGL"
     gene            2619..3161
                     /locus_tag="PK518_07035"
     CDS             2619..3161
                     /locus_tag="PK518_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167277.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRM48707.1"
                     /translation="MNRVPLIDRADTSAERKALLDAIHGAFGATPNMFRAVANSPAAL
                     KSMWGSFGALGGGTLGARLGEQIAVAVADRNACSYCLAAHTVLGQKAGASAEEMAAAQ
                     LGESSDPKTRAALLFALKVVGQRGQVSGADVQALRDAGFSDEQVVEILAHVALNLFTN
                     YVNLAFAVPVDFPAVKLSSG"
     gene            complement(3168..4016)
                     /locus_tag="PK518_07040"
     CDS             complement(3168..4016)
                     /locus_tag="PK518_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRM48708.1"
                     /translation="MRSTASGQAAMPAPDPSMPEVVDQVALARLYGEPLFALPQDLYI
                     PPDALEVFLEAFEGPLDLLLYLIRKQNFNILDIPMVDVTRQYLGYVDEVRSRNLELAA
                     EYLLMAAMLIEIKSRMLLPPKRQEGAAEPEDPRAELVRRLLEYEQMKQAAMRLNQVPQ
                     YGRDFIRAQVYIEQSLQPRFPDVDLAELQQAWRDIMRRAKLVQHHRITREELSVREYM
                     SQVLKTLQGRRFVPFEDLFQPEKGGTVLVVTFIALLELAKETLIEITQAEAFAPIYVR
                     LAYTPV"
     gene            complement(4086..4307)
                     /locus_tag="PK518_07045"
     CDS             complement(4086..4307)
                     /locus_tag="PK518_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HRM48709.1"
                     /translation="MNIFRRPDYQSEVTQFIGQLKDQKPALDAQQQAGRALLWDKEVD
                     REVWAEYRAGRVAQKPYVYFENFRKPGSF"
     gene            4354..4854
                     /locus_tag="PK518_07050"
     CDS             4354..4854
                     /locus_tag="PK518_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRM48710.1"
                     /translation="MNPLTRITGALAAGALALMSLAGCDDQRIRELEEDVSTEQDVRA
                     RFGEPERIWPEPDGSQTLEYNRQPAGHRNYMITIGPGGKMTALRQVLAPHNFEKVQPG
                     MDELAVRRLLGKPAKRMTFDLKQETDWDWNWIDPPNREMIFTATFGPDGRVKRSGSVE
                     KLPEGR"
     gene            complement(4858..5106)
                     /locus_tag="PK518_07055"
     CDS             complement(4858..5106)
                     /locus_tag="PK518_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48711.1"
                     /translation="MTKPCLSRPRALGAFLCLAALLPGCAVVAVTATAVSITASAVGL
                     AADAAVGTAKLAGKGVGMTVDALRDEAPRDDASGVEIR"
     gene            complement(5107..7173)
                     /gene="metG"
                     /locus_tag="PK518_07060"
     CDS             complement(5107..7173)
                     /gene="metG"
                     /locus_tag="PK518_07060"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRM48712.1"
                     /translation="MSPRKLFVTTALPYANGNFHIGHIMEYIQADTWVRAQRMQGAEV
                     NFVGADDAHGAPIMIAAEKAGKTPQQFVADIAAGRKQYLDGFHIAFDNWHSTDAPENH
                     QLAQQIYLDLKAAGLIETRTIEQFFDPEKNMFLPDRFIKGECPRCHAKDQYGDNCESC
                     GAVYAPTELIKPYSALSGAQPELKTSEHFFFKLSDPRCVAFLQEWTQNGVHVQAEVAA
                     KIKEWFGTRTNPDGSTSSGLDDWDISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASL
                     KNLLEKRGESYDAYMADPDLEQVHFIGKDIITFHTLFWPAMLKFSGRKTPTKICVHGF
                     MTVNNGEKMSKSRGTGLDPLKYLALGMNPEWLRYYLGAKLNGRNEDIDFNASDFMLRV
                     NSDLIGKYVNIASRAAGFIAKRFDGRLGQVSEDGQALLATLRAQKDTIIAAYEARDTA
                     RAAREAMQLCDRVNEYVDANKPWELAKKDGMGERLHDVCSTCIEAFRILTIYLKPMLP
                     RLAADVASFLIVPPEQFADVNKPLGAGHQIGAYQHLMQRVDEKQLEALFEMPEPVVEK
                     VTPGGEEIAPTIGIDDFAKVDLRIAKIVECKAVEGSTKLLQLTLDAGEGKTRNVFSGI
                     ASMYQPEDLQGKLTVLVANLAPRKMKFGVSEGMVLAASHADEKQHPGIYVLEPFPGAQ
                     PGMRIR"
     gene            complement(7236..8153)
                     /locus_tag="PK518_07065"
     CDS             complement(7236..8153)
                     /locus_tag="PK518_07065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM48713.1"
                     /translation="MNPQNGHFTLLIVDDDKHNRLLLTELFEGEYKIIQAKNGLQALE
                     LARAHAPDLILLDVLMPEMDGMAVIRALKRDDATRHIPVIFITALDSAADEELGLDLG
                     AVDYIAKPFHPPIARVRVRNHLQIVHQRRLLEQIAALDGLTGIPNRRRFDEALAQEWS
                     RCQRSGLPLSLIVADVDQFKQYNDTLGHAAGDRVLQDVAAALRQAARRPGDLAARYGG
                     EEFVLLLPETDAPQAQQLADELLQRMAARKLPHPAASAAPCVTLSLGGITTIPASSEV
                     APDFFERADAALYQAKAQGRNRWCWAPAT"
     gene            complement(8150..10765)
                     /locus_tag="PK518_07070"
     CDS             complement(8150..10765)
                     /locus_tag="PK518_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM48714.1"
                     /translation="MNALLQRLRPRSIRSRLLWWFTGAIVLALCLVYLLALLQQQRLI
                     RSEWSAGLRAQALLIATNSQAAVDFQDRQEAARLLQAVQSSPSVLRARLLLAGRREPF
                     AEFARQGSAVAATPSPPLDTQDGQDMHFGNNQVVAWAAVPGSAGKAHVELVASTQDMQ
                     QVLWRTALETGGALLALLLLLLWLSGRAARRLAVPLQNLNQLMARAADNPGLGERVAS
                     HGEDELAQLGRSLNQMIDKLQARDQELARYRQNLEQLVDQRTHALIAATEQAQQASRA
                     KSDFLARMSHEIRTPMNAIVGLGQLLLKTGLNAHQRDYQQKVLAASDMLLALINDILD
                     YSRIEAGRLQIEAIDFDLEQVLQGVSDQLALRAQQKGLALRFVTGAGVPRRLRGDPLR
                     LSQVLVNLCNNAVKFTESGEVVVQTELREQQGDRAVLEFCVRDTGMGIPADRLAQLFT
                     PFTQVDGSITRRFGGSGLGLAICHQLVELMGGTIAVQSQEGAGSQFSFTLPLAPAAPE
                     PARSASHGDAVAQGDHSLLRGRRVLLVDDVELNRTVALAFLAEAGVLVDVAVHGREAV
                     DKVLAQPYDLVLMDIQMPEMDGLTATREIRQQAHLQGLPIIAMTAHAMAGDRERSLQA
                     GMQDHLTKPIHPDALYAALRRWMAPRSSQGAGHTGAEPAPAAESAAQIPALEGVDTAR
                     GLAQALGRPGLYLRVLAQFVQDFGASAQAMQDAQAAHDWPLARRLAHSLKSGSATIGA
                     TALAQQARQLEDGFAQERPAEAMLLAGVGTELARICSLLAPLVAQAQPTGQAIAQAPP
                     TPLQPLLAELRNLLENDDAAALRLLQQLQSQAAAHPPLARTLPALRALVEDIEYQDAL
                     AQLRSLCAELEQNPT"
     gene            complement(10762..11295)
                     /locus_tag="PK518_07075"
     CDS             complement(10762..11295)
                     /locus_tag="PK518_07075"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HRM48715.1"
                     /translation="MIVQLLVALMPLALLAGGARAQQEPISEYAVKAALLVKLPRFVY
                     LPRLEGGTRISLCVLGRNPFGGALTRLAQTPMDGRSVQVSQADDASQVLDCDFVFVAR
                     SEADHLRSTLRQLGRAQVVTVSDIDGFARAGGMVELAVNPEGGNLLNILINRKAAQAQ
                     NIQFNAQLLRLARVVEP"
     gene            complement(11577..13829)
                     /locus_tag="PK518_07080"
     CDS             complement(11577..13829)
                     /locus_tag="PK518_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRM48716.1"
                     /translation="MDTDKKHCLAAALALCGLACPPLWAQGQLESDAQRLMGLSLQDL
                     IATPVVTASRQIETRDQTPAQILVITREQIRERRYRSLADLMRDLPGVDWQGGTKSSQ
                     FNQFAVQGYVGPNKLLILMDGVRIGAPAGGNFPVADNLALYMARQVEVLYGPAAALYG
                     ADAVAGVINIITEAGQGPRGSWASVGAGRFGGEEGAFMTGLDTAQGLQLSLGGHWQRA
                     ERAPLARYYPREFAKVPAVGNGQTIIPVDQREPYVGPTASHSLYARADWDERFTVGFF
                     RHSFTHLTSTGDPPAMARYQQSSEWQPTSDTFHGRWRFQPAPDVQAQLLVEHSRMELD
                     PKARYNNTFSNYQDSFSYELGKRTGLEQSLNWRISERQQLQAGLGWHQYHAEAASSLA
                     APYRPGLAAHGQGQLYPNTPLPRQIYDDNFRNLSAYAQLQSQWSGAFSTSAGLRLDRH
                     SHYGSSLNPRLGAVYKPREQHVFKLQYGEAFRAPSPEETLSAYGTFSGAQDAQGNYIG
                     SNFRIPNFNLQPEKARTLSAAWDWRPTPSLNLVTHLYHSRISRLVVTQPSGNVGAIPG
                     AVLINPETKGNAGRQQQSGLDLSAQWRFRLGGDWTGDLWGSASWIHGRIDEGNGVDWA
                     IPYVASHKLKLGTTLRWRNQVSITPQLYWTGNVTNGRKQGPTDPLLPQGACQGAMTAP
                     SRCSTPGYALVDLHLGWHQLLDGRATLWLDVYNLLDRRHYAAAGSGSRTFWDMPQQPR
                     TWMLTLDWRY"
     gene            complement(14070..15323)
                     /locus_tag="PK518_07085"
     CDS             complement(14070..15323)
                     /locus_tag="PK518_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRM48717.1"
                     /translation="MPHASPPDGSTALVLSGGGARAAYQVGVLQAIAGLRAACGHGHG
                     GAPFDIFSGTSAGAINAAALASGADHFDHAVRRIARAWAHVHAQQVYHADSLHVIQRG
                     AHWLTLLSLGWALARWRRVHPHSLLNNAPLAQLLEKLVPLQRVPHLIRAGHLRALAVT
                     ASSYNSGLHVAFFDAGDAQEPWVHSLRKSARGPITHAHLLASSAIPLVFPATALPIDG
                     HTEYFGDGSMRETSPLAPAIHLGARRLLVVGTSRAYEPPPAVPVGTQQPYPSLAKVAG
                     HALSNIFLDALAVDVNQVQHINQALALLTPEQRSRSPMHPLELLVFVPSRSLDEEAAR
                     HVCDLPRSMRVLLSALGVPVNRPGDSDGALASYLLFERAYTRRLMGLGRSDALARRDE
                     ICAFFGWQDGGDPLRLVQYSTLSGT"
     gene            complement(15335..15910)
                     /locus_tag="PK518_07090"
     CDS             complement(15335..15910)
                     /locus_tag="PK518_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRM48718.1"
                     /translation="MAPNSGFAILLRSPWWVSFAIAAVIVLLCGALLPAHLAPFAAVG
                     ALPVAVIGCIAGWRQWRAPSAARVQAVLQQAAAMPWRQFAQRLEDAWRAEGHEVQRMN
                     GPHCDLRITRGEQTLLVAARRYKAATHGVEPLRELQAEVQRQGARAGVYVALQGAVSE
                     QARGFARDNALALLEGDALATLLLKATPPKT"
     gene            complement(16194..16658)
                     /locus_tag="PK518_07095"
     CDS             complement(16194..16658)
                     /locus_tag="PK518_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HRM48719.1"
                     /translation="MDTLSLDASIAITGISRSTLWRRVTDGSIGRGDKDGRSRAMLAL
                     GDVLALVEVKLSAEDRAMLLRADAGDAQAQADMGALFYVAGAHKAALYWLQEAAAQDN
                     ADAMQWLGTAYAGGGGVIPQDDNLAIMWLARAAALGHRIAAYQLEQLRAGCR"
     gene            16894..17376
                     /locus_tag="PK518_07100"
     CDS             16894..17376
                     /locus_tag="PK518_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRM48720.1"
                     /translation="MKSNMMRRAQAGFTLIELMIVVAIIGILAAVALPAYQDYTVKSK
                     WAANLAEVEGIKTAIKTCMNDEASDGLKCDTVAELNNYGFPGTVLPTPRYATAALTLT
                     GAAAVAPASGSVTIKFTGTSDVKSLVYEGKCEVNTGGNIVCEKTASDTLGQYVKTTGR
                     "
BASE COUNT         2747 a         5755 c         6117 g         2984 t
ORIGIN      
        1 tcagcgcagc ggcggcacca ggatggtggg cgccgcgggc gcggccatgt cgggccagtc
       61 gcgactgtag tgcaggccgc ggctctcatg gcgcatctgg gcgctttgca cgatcaggtc
      121 ggccacctgc accaggttgc gcagctccag caggtcacgc gtgacgtgga agtgggcgta
      181 gaactcgtgg atctcgccct tgagcagcgc gatgcggtgc gccgcgcgct ccagccgctt
      241 gttggtgcgc actatgccga cgtagtccca catgaagcgg cgcagctcgt cccagttgtg
      301 cgagatgacg accgattcgt cggcgtcgcc cacgcggctg tcgtcccagg ggggcagggc
      361 gggcgacttg gcgggctgcg cctgggtgat gtagctgcac gcggcgcgcg cgaacaccag
      421 gcattccagc agcgagttgc tggccaggcg gttggcgccg tgcaggccgg tgtaggccac
      481 ctcgcccacg gccagcaggc ccgccaggtc ggtgcggccc gacaggtcgg tgagcacgcc
      541 gccgcaggtg aaatgcgcag tgggcacgac ggggatcggc tcccgggtga tgtctatgcc
      601 gagggctgcg caatgggcca ggatgttggg gaaatgctcg cgcagaaact ccgggctctg
      661 gtgcgagatg tccaggtgca cgcaatcgag gccgtgtttc ttcatctcga agtcgatggc
      721 gcgcgccacc acgtcgcgcg gggccagctc ggcgcgcgca tcgtgcgcgg gcatgaagcg
      781 cgtgccatcg ggcagcttga gttgcccgcc ctcgccgcgc acggcctcgc tgatcagaaa
      841 ggatttctcg tgcgggtggt acaggcccgt ggggtggaac tgcacgaact ccatgttgcc
      901 cacgcggcag cccgcgcgcc aggccgcggc aatgccatcg cccgtggcgg tgtcggggtt
      961 ggtggtgtag aggtagacct tgcccgcgcc gccggtcgcc aggatggtgt gcggcgcgcg
     1021 gaaggtgacc acgctgtcac tggcctggtc cagcgcgtac aggcccacgc agcgcggctg
     1081 gccggccagg cccagcttgg cggtggtgat caggtccacc agcgtgtgct gctcgaacac
     1141 cgtgatgccg ggcgtggcgc gcaccacctc ggtgagtgtc ttttgcaccg ccgcgccggt
     1201 ggcgtcggtg gcatgcacga tgcgccgcgc gctgtgccca ccctcgcgcg tcaggtgcag
     1261 gccgctgccc tccagcgtga agggcacgcc cagggcgcgc agccagccta tggcggcggg
     1321 tgcgttctcg accacgaagc gggtggtggc catgtcgcac aggccggcac cggccaccag
     1381 cgtgtcctgg atatgggcgg caaagctgtc gtcatcggcc agcacggcgg caatgccgcc
     1441 ctgggcccag ttgctggcgc cgtccttcaa ctcgcgcttg gtgatcacgg ccacgcggtg
     1501 cgtgggcgcc agttgcaggg ccgcggacag gccggccagg ccgctgccta cgatgagtac
     1561 gtcgaaatca tgcgatgcca tggtggtctg ccgcaagcgg gcgcgcgaag ggaggctgca
     1621 ttgtagggag ccgccggccc gctccggtca caagcccttg agccactcgc gcggcgccgt
     1681 ccctaccgcc tgggcaaacg cccgggtgaa tgccgccggg ctggcgtagc ccagctggtc
     1741 gctgatctgc cgcacctgca gcccgcgccg cagcaggccc tgggccagcg acacgcgcca
     1801 ggccagcagg taggcgccgg gcgtggtgcc cagcgcggcg cggaactcgg cggcaaaggc
     1861 gctgcgcgac atgccggcgg cctgcgccat ggtggccagg ctccagttgg cgccgggctg
     1921 ctcgtgcagc cgggtgaggg cgcgcgctaa ttttgggtgg gccaggcccg ccagcaggcc
     1981 gggctgcatg ccgccatcgg cgggatggtc gagcagccag cgcagcagct gcagcagcaa
     2041 cacctcgaac aggcgatcgg ccaacaggcg ctggccgcag cgcacgcgct cggtctcggc
     2101 aaacagcagg gtcagggtct gctctatgcc gtcgacctcg gcaatcggca gggccagaaa
     2161 ggcgggcagg gcctgcacca gcgggtggtg tggcccgccg tcaaagtcca gcgtggcgca
     2221 gacgaagtcg ctgccctctt gcggcgcgtt gtaaaagtcg tgctccagcg ggcgcgggta
     2281 cagcagcagc gtgggctggt tcaggcgcag ccggcgcggc gcgccgctgc gcgggcggtg
     2341 ggtgacctcc agctcgccgc ggcgcagcac atgcagaaaa gcgcgcccgg gctcggccgc
     2401 gaacggcgtc acgccgcaca gcggcccggc gtggaagagg tgggcgcgca cgcgaaaacg
     2461 ctccagcagg gcggagaggc gatccacgcc ggcggcggcc gtatcggtct gaatcatgga
     2521 cgattggcaa agtatcgcgg actgaatgcg tccaatcata tgaattgccg gcgcaaactg
     2581 ccagcatgtt tcactgccca gccgcaagga ggtttgccat gaaccgcgtt cccctcatag
     2641 accgtgccga caccagtgcc gagcgcaagg ccctgctcga cgccatccac ggcgcctttg
     2701 gcgccacgcc caacatgttc cgcgccgtgg ccaattcgcc ggccgcgctc aagagcatgt
     2761 ggggctcgtt cggcgccctg ggcggcggca cgctgggcgc caggctgggc gagcagatcg
     2821 ccgtggccgt ggccgaccgc aatgcctgct cttactgcct ggcggcgcat accgtgctgg
     2881 gccagaaggc cggcgccagc gccgaggaaa tggccgccgc ccagctgggc gagtccagcg
     2941 accccaagac ccgggccgcg ctgctttttg ccctcaaggt ggtgggccag cgcggccagg
     3001 tgagcggcgc cgacgtgcag gccctgcgtg atgccggttt cagcgacgag caggtggtcg
     3061 agatcctggc ccatgtggcg ctgaacctgt tcaccaacta cgtgaacctg gcctttgccg
     3121 tgccggtgga ctttccagcg gtcaagctga gcagcggctg atgggcttca gacgggtgta
     3181 taggccaggc gcacgtagat cggggcaaag gcctcggcct gggtgatctc gatcagggtc
     3241 tccttggcca gctccagcag ggcgatgaag gtgaccacca gcacggtgcc gcccttctcg
     3301 ggctggaaca ggtcttcaaa cggcacgaag cgccggccct gcagggtctt gagcacctgg
     3361 ctcatgtact cgcgcaccga tagctcctcg cgcgtgatgc ggtggtgctg caccagtttg
     3421 gcgcggcgca tgatgtcgcg ccaggcctgc tgcagctcgg ccaagtccac gtcgggaaag
     3481 cgcggctgca ggctttgctc tatgtagacc tgggcgcgga tgaaatcacg cccgtactgc
     3541 ggcacctggt tcaggcgcat ggcggcctgc ttcatctgct cgtattccag caggcggcgt
     3601 accagctcgg cgcgcgggtc ctcgggctcg gccgcgccct cctgccgctt cgggggcagc
     3661 agcatgcgcg acttgatctc tatgagcatg gccgccatga gcaggtattc ggccgccagc
     3721 tccaggttgc ggctgcgcac ctcgtccaca tagcccaggt actggcgcgt gacatccacc
     3781 atggggatgt ccaggatgtt gaagttctgc ttgcgtatca ggtacagcag cagatccagc
     3841 ggcccctcga aggcctccag gaacacctcc agcgcgtcgg gcgggatgta caagtcctgg
     3901 ggcagggcga acagcggctc gccatacagg cgcgccaggg ccacctgatc gaccacctcg
     3961 ggcatggagg ggtcgggcgc cggcatggcc gcttgcccgg aggcggtaga gcgcattacg
     4021 ctatttaatt aatagctatt gacgcatgct gtgcaagcgg tatggccggt tttgcctgat
     4081 gctggttaaa aggaacccgg tttgcggaag ttctcgaaat acacgtaggg cttttgcgcc
     4141 acgcgcccgg cccggtactc ggcccaaacc tcgcgatcga cctccttgtc ccacagcagg
     4201 gcgcggccgg cctgttgctg cgcgtccagc gcgggctttt gatccttgag ctggccgatg
     4261 aactgggtga cttcggactg gtagtcgggg cggcggaata tattcatgga tggtttacct
     4321 ctcagaaccc gcgattttaa cgatacccac cccatgaacc ctttgacccg catcaccggc
     4381 gcgctggccg ctggcgccct ggccctcatg tcgctggcag gctgcgacga ccagcgcatc
     4441 cgcgagctgg aagaagacgt ctccaccgag caggacgtgc gcgcccgttt tggcgagcct
     4501 gaacgcatct ggcccgagcc ggacggcagc cagacgctgg agtacaaccg ccagcccgcc
     4561 ggccaccgca actacatgat caccataggc ccgggcggca agatgacggc gctgcgccag
     4621 gtgctggcgc cgcacaactt cgagaaagtg cagccgggca tggacgagct ggccgtgcgc
     4681 cgcctgctgg gcaagccggc caagcgcatg accttcgatc tgaagcagga gacggactgg
     4741 gactggaact ggatcgaccc gcccaaccgc gaaatgatct tcaccgcgac ctttggcccc
     4801 gatgggcgcg tcaagcgctc gggcagcgtg gagaagctgc ccgaagggcg ttgaaggtca
     4861 gcgtatctcc acgccgctgg cgtcgtcccg tggcgcctcg tccctgagtg cgtccacggt
     4921 catgccaaca cccttgccgg cgagttttgc cgtacctacg gcggcatcgg ccgccaggcc
     4981 caccgccgag gctgtgatgc tgacggccgt tgccgtcacg gccaccaccg cgcagcccgg
     5041 cagcagggca gccaggcaca aaaaagcccc cagggcccgg gggcgtgaga ggcaaggctt
     5101 ggtcattcag cggatgcgca tgcccggttg ggcgccgggg aagggctcca gcacgtagat
     5161 gccgggatgc tgcttttcat ccgcgtggct ggcggccagc accatgccct cagagacgcc
     5221 gaacttcatc ttgcgcggcg ccaggttggc caccagcacc gtcagcttgc cctgcaagtc
     5281 ttccggttgg tacatgctgg cgatgccgct gaacacattg cgtgtcttgc cttcaccggc
     5341 gtccagcgtc agctgcagca gcttggtcga gccctccacc gccttgcatt cgacgatttt
     5401 cgcgatgcgt aagtccacct tggcgaagtc gtcgatgccg atggtgggcg caatctcctc
     5461 cccgccgggt gtgacttttt cgaccaccgg ctcgggcatt tcaaacagcg cttccagctg
     5521 cttttcgtcc acgcgctgca tcaggtgctg gtaggcaccg atctggtggc cggcgcccag
     5581 cggcttgttg acatcggcaa actgctcggg cggcacgatc aggaagctgg ccacgtcggc
     5641 ggccagccgg ggcagcatgg gtttcaagta aatcgtcagg atgcggaagg cctcgatgca
     5701 ggtgctgcac acgtcgtgca ggcgctcgcc catgccgtcc tttttggcca gctcccaggg
     5761 cttgttggca tcgacatatt cgttcacgcg gtcgcacagc tgcatggcct cgcgcgcggc
     5821 gcgtgccgtg tcgcgcgcct cgtaggcggc gatgatggtg tctttctgcg cgcgcaaggt
     5881 ggccagcagc gcctggccgt cctcgctcac ctggcccagg cggccgtcaa agcgcttggc
     5941 gatgaagccg gcggcgcgtg acgcaatgtt gacgtacttg ccgatcaggt cggagttcac
     6001 gcgcagcatg aagtcactgg cgttgaagtc gatgtcttca ttgcggccat tgagcttggc
     6061 gcccaggtag tagcgcagcc attcggggtt catgcccagc gccaggtatt tgagcgggtc
     6121 caggcccgtg ccgcggctct tgctcatctt ctcgccgttg ttcacggtca tgaagccgtg
     6181 cacgcagatc ttggtgggcg tcttgcggcc gctgaacttc agcatggcgg gccagaacag
     6241 cgtgtggaag gtgatgatgt ccttgccgat gaagtggacc tgctccaggt cggggtcggc
     6301 catgtaggcg tcgtagctct ctccgcgctt ttccagcagg tttttcagcg acgccagata
     6361 gcccaccggt gcgtccagcc acacatagaa atacttgccc ggcgcgtccg gtatctctat
     6421 gccgaagtag ggcgcgtcgc gcgagatgtc ccagtcgtcc aggccgctgc tggttgagcc
     6481 gtcggggttg gtgcgggtgc cgaaccactc cttgatcttg gccgcgacct cggcttgcac
     6541 atggacgccg ttttgcgtcc attcctgcaa gaacgccacg cagcgcgggt cggacagctt
     6601 gaagaagaaa tgctccgagg ttttcaactc gggctgggcg ccggacagcg cggaatacgg
     6661 cttgatcagt tcggtgggcg catacacggc gccgcagctc tcgcagttgt cgccgtactg
     6721 atccttggcg tggcagcgcg ggcattcgcc cttgatgaag cggtccggca ggaacatgtt
     6781 cttctcgggg tcgaagaact gctcaatggt gcgcgtttcg atcaggccag cggccttcag
     6841 gtccagatag atttgctgcg ccagctggtg gttctcgggc gcgtcggtgc tgtgccagtt
     6901 gtcaaaggcg atgtgaaagc cgtccaggta ctgcttgcgc ccggcggcga tgtcggccac
     6961 gaactgttgc ggcgtcttgc cggccttctc ggcggcgatc atgatgggcg cgccatgagc
     7021 gtcgtcggcg cccacgaagt tcacctcggc gccctgcatg cgctgcgccc gcacccaggt
     7081 gtcggcctgt atgtattcca tgatgtggcc gatgtggaag ttgccgttgg catacggcag
     7141 ggcggtggtg acgaagagct tgcgtgggga catggactga aactcgggct ggctgaagca
     7201 tggggcgtgc gtcggcacgg acggaacccg cgattttagg tcgccggtgc ccagcaccag
     7261 cggttgcggc cctgcgcctt ggcctggtag agcgccgcat cggcacgttc aaagaaatcg
     7321 ggggctacct cgctgctggc cggaatggtg gtgatgccgc ccaggctcag ggtcacacag
     7381 ggcgcggcgc tggctgccgg gtgcggcagc ttgcgcgccg ccatgcgctg cagcagctca
     7441 tcggccagct gctgggcctg cggggcatcg gtttcgggca gcagcagcac gaactcctcg
     7501 ccgccatagc gcgccgccag gtcgccgggg cggcgcgcgg cctggcgcag cgcggcggcc
     7561 acgtcctgca gcacgcggtc gcccgcggcg tggcccaggg tgtcgttgta ctgcttgaac
     7621 tgatcgacgt cggcaacgat cagcgacagc ggcaggcccg agcgctggca gcggctccac
     7681 tcctgcgcca gggcttcgtc gaagcggcgc cggttgggga tgccggtcag gccgtcgagc
     7741 gcggcgatct gctccagcag gcgccgctgg tgcacgatct gcaggtggtt gcgcacgcgc
     7801 acgcgggcga tgggcggatg aaacggcttg gcgatgtagt ccacggcgcc cagatccagg
     7861 cccagttcct cgtcggcggc ggagtcgagc gcggtgatga agatcaccgg gatatggcgc
     7921 gtggcgtcgt cgcgcttgag cgcgcggatc acggccatgc cgtccatctc gggcatgagc
     7981 acgtccagca ggatcaggtc cggggcgtgg gcgcgcgcga gctccagcgc ctgcaggccg
     8041 ttcttggcct ggatgatctt gtactcgccc tcgaacagct cggtcagcag caggcggttg
     8101 tgcttgtcgt catcgacgat gagcagggtg aaatggccgt tctgcgggtt catgtggggt
     8161 tttgctccag ctcggcgcac agcgagcgca gctgcgccag tgcgtcttgg tattcgatgt
     8221 cctccaccag cgcgcgcagc gccggcagag tccgggccag cggggggtgg gcggcggcct
     8281 ggctctgcag ttgctgcagc aggcgcagcg cggcggcgtc gtcgttttcc agcaggttgc
     8341 gcagctcggc caacagcggc tgcagcggcg ttgggggcgc ttgcgcaatg gcttggcccg
     8401 ttggctgcgc ctgggcgaca agcggtgcca gcaggctgca gatgcgcgcc agctccgtgc
     8461 ccacgccggc cagcagcatc gcttcggccg ggcgctcctg ggcaaagccg tcttccagct
     8521 gcctggcctg ttgtgccaag gctgtggcgc ctatggtggc gctgcccgat ttgagcgaat
     8581 gcgccaggcg ccgcgccagg ggccagtcat gcgcggcctg cgcgtcctgc atggcctggg
     8641 cgctggcgcc gaagtcctgc acgaactgcg ccagcacgcg caggtacagc ccgggccgcc
     8701 ccagggcctg ggccaggccg cgcgctgtgt ccacgccctc cagggcgggg atttgcgcgg
     8761 cggattcggc ggccggtgcc ggctcggccc cggtgtggcc cgcgccctgg ctactgcggg
     8821 gcgccatcca gcgccgcagc gccgcgtaca gcgcatcggg gtggatgggc ttggtcaggt
     8881 gatcctgcat gccggcctgc aggctgcgct cgcggtcgcc cgccatggcg tgggcggtca
     8941 tggcgatgat gggcaggccc tgcaggtgcg cctgttggcg gatctcgcgc gtggccgtga
     9001 ggccgtccat ctccggcatc tggatatcca tcagcaccag gtcgtagggc tgggccagca
     9061 ccttgtccac ggcctcgcgg ccatgcacgg ccacatccac cagcacgccg gcctcggcca
     9121 ggaaggccag ggccacggtg cggttgagct ccacgtcgtc caccagcagc acgcgacggc
     9181 cgcgcagcag gctgtggtcg ccctgtgcca cggcgtcgcc atggctggca gagcgcgcgg
     9241 gctctggcgc cgccggtgcc aggggcaggg tgaaggagaa ctggctgccg gcgccctcct
     9301 ggctctgcac ggcaatcgtg ccgcccatca gctccaccag ctggtggcaa atggccaggc
     9361 ccaggccgct gccgccaaag cggcgggtga tggagccgtc cacctgggtg aagggcgtga
     9421 acagctgcgc caggcgatcc gcggggatgc ccatgccggt gtcgcgcacg cagaactcca
     9481 gcaccgcccg gtcgccctgc tgctcgcgca gttcggtctg caccacgacc tcgccagatt
     9541 cggtgaactt cacggcgttg ttgcacaggt tgaccagcac ctgggacagg cgcagcgggt
     9601 cgccgcgcag ccgccgtggt acgccggcgc cggtgacaaa ccgcagcgcc agcccctttt
     9661 gctgggcgcg cagcgccagc tggtcagaga cgccttgcag cacctgctcc aggtcgaagt
     9721 caatggcctc gatctgcagc cggccggcct cgatgcgcga gtagtccagg atgtcgttga
     9781 tcagggccag cagcatgtcg gaggcggcca gcactttctg ctggtagtcg cgctggtgcg
     9841 cgttcaggcc ggtcttcagc agcagctggc ccaggccgac gatggcgttc atcggggtgc
     9901 ggatctcgtg gctcatgcgc gccaggaagt ccgacttggc gcggctggcc tgctgggcct
     9961 gttcggtggc ggcgatcagg gcgtgggtgc gctgatccac cagctgctcc aggttctggc
    10021 ggtagcgcgc cagctcctgg tcgcgtgcct gcagcttgtc gatcatctgg ttcaggctgc
    10081 ggcccagctg cgccaactcg tcctcgccat ggctggccac gcgctcaccc aggccggggt
    10141 tgtcggccgc acgcgccatg agctggttca ggttctgcag cggcaccgcc aggcgcctgg
    10201 cggcgcggcc cgagagccac agcaggagca gcagcagggc cagcagcgcg ccgccggtct
    10261 ccagtgccgt gcgccacagc acctgctgca tgtcctgcgt gctggccacc agttcgacat
    10321 gcgccttgcc ggcgctgccc gggacggccg cccaggcaac gacctggttg ttgccgaaat
    10381 gcatgtcctg gccgtcctgc gtgtccagcg gcgggctggg cgtggcggcc acggcgctgc
    10441 cctggcgcgc aaactcggca aagggctccc tgcggccggc cagcagcagg cgcgcgcgca
    10501 gcaccgaggg gctggactgc acggcctgca gcaggcgcgc ggcctcctgg cggtcctgga
    10561 aatccaccgc cgcctggctg ttggtggcaa tcaggagcgc ctgggcgcgc aggccggcgc
    10621 tccattcgct gcggatcagg cgctgctgct gcagcagcgc cagcaggtag accaggcaca
    10681 gggccagcac gatggcgccg gtgaaccacc acagcagccg gcttctgatg ctgcgcgggc
    10741 gcaggcgttg cagcagggcg ttcatggttc gacgacccgg gccaggcgca gcagctgcgc
    10801 attgaactgg atgttctgcg cctgcgccgc cttgcggttg atgaggatgt tcaacaggtt
    10861 gccgccctcg gggttcacgg ccagctccac catgccgccg gcgcgtgcaa agccgtcgat
    10921 gtccgacacc gtcaccacct gggcccggcc gagctgccgc aaggtgctgc gcaggtgatc
    10981 ggcctcgctg cgcgccacga agacgaagtc gcagtccagc acctggctgg cgtcgtcggc
    11041 ctggctgacc tgcacgctgc gcccgtccat gggcgtttgc gccagcctgg tcagtgcccc
    11101 gccaaagggg ttgcgcccca gcacgcacag gctgatgcgg gtgccgccct ccaggcgtgg
    11161 caggtagacg aagcgcggca gtttgaccag cagcgccgcc ttgaccgcgt attccgagat
    11221 cggctcctgc tgcgcccgcg cgccaccggc gagcaaggcc agcggcatca gcgccaccag
    11281 cagctggaca atcacttttt gatagctgct aacgcttgat gtataagcgc tggaggccat
    11341 ttttacctga ctatgtacat gccgctgatg cccctcaccc ctccctctcc ccaaaggggc
    11401 gagggcgacc ggttgcgccg gctttactcc ctctccctct gggagagggt gggggtgagg
    11461 gctcgcggcc cagtctgaga catgcgcctt gtcaggccct tttaaccaaa aaccgatctt
    11521 gcgcgccgtg gctgccatgg ccggcgcttg cggccgaaaa cggtatgtcg gcacgctcag
    11581 taccgccagt ccagcgtcag catccaggtg cgcggctgct gcggcatgtc ccagaaggtg
    11641 cgcgagcccg agcccgccgc ggcgtagtgg cgcctgtcca gcaggttgta cacatccagc
    11701 cacagcgtgg cgcggccgtc cagcagctgg tgccagccca ggtgcagatc caccagcgca
    11761 tagccaggcg tgctgcagcg gctgggggcg gtcatggccc cctggcaggc gccctggggc
    11821 agcagcgggt cggtcgggcc ctgcttgcgg ccattggtca cgttgcccgt ccagtagagc
    11881 tggggcgtga tgctgacctg gttgcgccag cgcagcgtgg tgcccagctt gagcttgtgg
    11941 ctggccacat aggggatggc ccagtccacg ccattgccct catcgatgcg gccatggatc
    12001 cagctggcgc tgccccacag gtcgccggtc cagtcgccac ccaggcgaaa gcgccactgc
    12061 gccgacagat ccaggcccga ctgctgctgg cgcccggcat tgcccttggt ctcggggttg
    12121 atcagcaccg cgcccgggat ggcccccacg ttgcccgagg gctgcgtgac caccaggcgg
    12181 ctgatgcggc tgtggtagag gtgcgtgacc aggttcaggc tgggcgtggg gcgccagtcc
    12241 caggcggcgc tcagcgtgcg cgccttctcc ggctgcaggt tgaagttggg aatgcggaag
    12301 ttgctgccga tgtagttgcc ctgcgcgtcc tgtgcgccgc tgaaggtgcc gtaggcgctc
    12361 agcgtttcct cgggcgacgg ggcgcggaat gcctcgccgt actggagctt gaagacatgc
    12421 tgctcgcgcg gcttgtagac ggcgcccagg cgcgggttga ggctgctgcc gtaatggctg
    12481 tggcgatcca ggcgcaggcc ggcgctggtg ctgaaggccc cgctccactg cgactgcagc
    12541 tgcgcatagg ccgagaggtt gcggaagttg tcgtcgtata tctgcctggg cagtggggtg
    12601 ttggggtaga gctggccctg gccatgggcg gccaggccgg gcctgtaggg cgcggccagg
    12661 gacgaagccg cctctgcatg gtattggtgc cagcccaggc cggcctgcag ctgctggcgc
    12721 tcgctgatgc gccagttcag gctttgctcc aggcccgtgc gtttgcccag ctcgtaggaa
    12781 aagctatcct ggtaattgct gaaggtgttg ttgtagcgcg ccttggggtc gagctccatg
    12841 cgcgaatgct ccaccagcag ctgcgcctgc acatcgggcg ccggctgaaa gcgccagcgg
    12901 ccatggaagg tgtccgaggt gggctgccac tcggagcttt gctggtagcg cgccatcgcc
    12961 ggcgggtcgc cggtgctggt caggtgggtg aagctgtggc gaaagaagcc cacggtgaag
    13021 cgctcgtccc agtcggcgcg cgcatacagg ctgtggctgg cggttgggcc cacatagggc
    13081 tcgcgctggt cgacggggat gatggtctgg ccgttgccaa cggccggcac cttggcaaac
    13141 tcgcgcgggt agtagcgtgc caggggcgcg cgctcggcac gctgccagtg cccgcccagt
    13201 gacagctgca ggccctgcgc ggtatccagg ccggtcatga aggcgccctc ctcgccgccg
    13261 aaacgccccg cccccaccga tgcccaggag cccctggggc cctggccggc ctcggtgatg
    13321 atgttgatca ccccggccac ggcgtcggcg ccatacagcg cggcggcggg gccgtagagc
    13381 acctcgacct gcctggccat gtacagcgcc aggttgtccg ccacgggaaa attgccgccc
    13441 gcgggcgcgc ctatgcgcac cccgtccatc aggatcagga gcttgttcgg ccccacatag
    13501 ccctgcacgg caaactggtt gaactgcgag gacttggtgc cgccttgcca gtccacgccg
    13561 ggcagatcgc gcatcaggtc ggccaggctg cggtagcgcc gctcgcggat ctgctcgcgc
    13621 gtgatgacca ggatctgcgc cggcgtctga tcgcgcgtct cgatctggcg cgaggccgtg
    13681 acgaccgggg ttgctatcaa atcctgtagc gacaggccca tcaggcgctg ggcgtccgat
    13741 tccagctggc cctgcgccca cagcggcggg caggccaggc cgcagagcgc cagcgcggca
    13801 gcaagacagt gcttcttatc cgtgtccatc gaagaggttg gctacatttt tgacaacttg
    13861 tcatgctatc agccgggtca ctccagccct gtcgcgcgcg gccaacaggg cgccgggcgg
    13921 cttcaggcga agcaggcgag tgcagtgttg gacgcttgat ggaacaaata aaaccgatgg
    13981 atttttggtc tgggctaggc gcaaaccgga gcgatagccg tagctatcgc gaggatttgc
    14041 aacgccgccc gggccgaaaa ggcgcggttt tatgttccag atagcgtcga atactgcact
    14101 agacgcagcg ggtcgccgcc gtcctgccag ccgaagaagg cgcagatctc atcgcgccgg
    14161 gccagcgcgt cgctgcgccc caggcccatc agccggcgcg tgtaggcgcg ctcgaacagc
    14221 aggtagctgg ccagcgcgcc atcgctgtcg cccggccggt tcaccggcac gcccagcgcc
    14281 gacagcagca cgcgcatgct gcgcggcagg tcgcagacat ggcgcgccgc ctcctcgtcc
    14341 aggctccgcg agggcacgaa caccagcagc tccagcgggt gcatggggct gcggctgcgc
    14401 tgctcgggcg tcagcagcgc cagtgcctgg ttgatgtgct gcacctggtt gacgtccacc
    14461 gccagcgcgt ccagaaagat gttggacagc gcatggccgg ccaccttcgc cagcgagggg
    14521 tagggctgct gcgtgccgac gggcaccgcc ggtggcggct cataggcccg gctggtgccc
    14581 accaccagca ggcggcgcgc gcccaggtgg atggcgggcg ccagtggcga ggtctcgcgc
    14641 atggagccgt cgccaaaata ctcggtatgg ccgtcgatcg gcagcgccgt ggccggaaac
    14701 accagcggga tggccgacga ggccagcaga tgcgcatgcg tgatgggccc gcgcgccgac
    14761 ttgcgcagcg aatgcaccca gggctcctgc gcatcgccgg cgtcgaagaa ggccacatgc
    14821 aggcccgagt tgtagctgga cgccgtcacg gccagcgcgc gcaggtggcc ggcgcgtatc
    14881 aggtgcggca cccgctgcaa tggcaccagc ttttcaagca gttgcgccag cggcgcgttg
    14941 ttcagcagcg aatgcgggtg cacgcggcgc cagcgcgcca gcgcccagcc cagcgacagc
    15001 aaggtgagcc aatgcgcgcc gcgctgtatg acatgcagcg agtcggcgtg atagacctgc
    15061 tgggcatgga catgggccca ggcgcgcgca atgcggcgca cggcatggtc gaaatggtcg
    15121 gccccgctgg ccagggccgc ggcgttgatg gcgccggccg aggtgccgct gaagatgtcg
    15181 aacggcgccc cgccatgccc atggccacag gccgcgcgca ggcccgcaat ggcctgcagc
    15241 acccccacct ggtaggccgc gcgtgcgccg ccgccggaga gcaccagggc ggtgctgcca
    15301 tcgggcgggc tggcgtgggg catggcggcg cctgctatgt cttgggcggc gtggccttga
    15361 gcagcagcgt ggcgagcgcg tcgccctcca gcagcgccag ggcgttgtca cgcgcgaagc
    15421 cgcgcgcctg ctcgctcacc gcgccctgca gcgccacata cacgccggca cgcgcgcctt
    15481 ggcgctgcac ctcggcctgc agctcgcgca gcggctccac gccatgcgtg gcggccttgt
    15541 agcggcgcgc ggccaccagc agggtctgct cgccccgggt gatgcgcagg tcgcagtgcg
    15601 ggccattcat gcgctgcacc tcatggccct cggcgcgcca ggcatcctcc agccgctggg
    15661 caaactggcg ccagggcatg gcggcggcct gctgcaaaac cgcctgcacg cgcgcggcgc
    15721 tgggcgcgcg ccactggcgc cagccggcaa tgcagccaat cacggccacc ggcagtgcgc
    15781 ccaccgcggc aaacggcgcc agatgcgccg gcagcagcgc accgcacagc agcacgatga
    15841 cggcggcaat ggcaaagctg acccaccagg gcgagcgcag caggatcgca aagcccgagt
    15901 tcggggccat cttgaatttc acgggttatt ccttgggtgt cttgtgcaca cccgcatttt
    15961 cctggtggcg tgtgccgcgt gtggggtgtt gcgttttact ccctcgcctc cttgggggag
    16021 agggtagggg tgagggggtg actggctgcg gcgcttgcct tcgccaccct cacccccact
    16081 ctctcccgcc agcaggagag ggagaaatgg ccgtaaggcc gcgcggaaac tgcgcaacat
    16141 gctatagaaa aatgaacaaa ccttggggct ggcgtccggc cggtcgcccg gccctaccgg
    16201 caccccgcgc gcagctgctc cagctgatag gccgcaatgc ggtgccccag cgcagccgcc
    16261 ctggccagcc acatgatggc caggttgtca tcctggggta tcacgccccc cccccccgca
    16321 taggccgtcc ccagccactg catggcatcg gcgttgtcct gcgcggcggc ctcctgcagc
    16381 cagtacagcg ccgccttgtg cgcgcctgcc acatagaaca gcgcgcccat gtcggcctgc
    16441 gcctgggcgt cgcccgcgtc ggcgcgcagc agcatggccc tgtcctcggc gctcaatttc
    16501 acctccacca gcgccagcac atcgcccagc gccagcatgg cgcggctgcg cccgtcctta
    16561 tcgccccggc caatcgagcc gtcggtcacg cgccgccaca gcgtgctgcg gctgatgccg
    16621 gtgatggcga tcgatgcgtc caggctcagg gtgtccatgt ctttgcgctc cttgtggccg
    16681 gcgattctat tgcgtgcccg ccaggccggc cctggcacgc gcgccggcat gccgtggcac
    16741 ggcaaatgct gtgtcaatcg gtggctggga tggggtgtga cgtttcaaga ccgtcagaaa
    16801 cacgatgaaa ccagtggaaa cgacttggca tgatcgttgc tgaatgtaag gcaaccggta
    16861 accgccggcc cattgttcaa ctggaggaat cttatgaaat ccaacatgat gcgccgcgct
    16921 caggccggtt ttaccttgat cgaactgatg atcgtcgtgg cgattattgg tattttggcg
    16981 gctgtggcgt tgccggctta tcaggattac acggtcaaat caaaatgggc agccaatttg
    17041 gccgaagtgg agggcatcaa aactgcaatt aaaacctgca tgaacgatga ggctagcgat
    17101 ggattaaaat gtgatacggt tgctgaactg aataactatg gcttcccagg tactgtattg
    17161 ccaacgccca ggtatgcaac cgctgcacta acgctaacag gtgcagccgc cgttgcccca
    17221 gcttcaggca gcgttaccat aaaattcact gggacgtctg atgttaaatc cctggtttac
    17281 gaaggaaaat gtgaagtcaa tactggcggc aacattgttt gtgaaaaaac cgccagcgat
    17341 acccttggtc agtatgttaa gactaccggt cgataatttg atacccaatt cgttggtgta
    17401 aattaatttt taaaacccgc cgaaaccatc ggcgggtttt atatttgcaa catctaatgt
    17461 gcaattgggg tcggactcta attaaacgca aacaaaaatt ggagctgaat tccaattgga
    17521 taattgataa ttggggtcag attccaatta attttggcct ccagcgcttg gtagtattgc
    17581 gttggtagct cttctttttg atg
//
LOCUS       DAOMTQ010000053        17331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_464_length_17331_cov_25.568882, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000053 DAOMTQ010000000
VERSION     DAOMTQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 17331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17331
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_464_length_17331_cov_25.568882"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..771)
                     /locus_tag="PK518_07105"
     CDS             complement(172..771)
                     /locus_tag="PK518_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRM48721.1"
                     /translation="MAYAGTQPGKNGSAKGSVILVVGDSLSAEYGLARGTGWVTLLEQ
                     RLAQEKIPATVVNASVSGDTTAGGRARLAALLKLHQPTHVVLELGSNDALRGLPLANT
                     QDNLDWMTEQAQAAGARVLLVGMQMPPNYGADYGRRFASLFEAVAKERKAALVPFLLK
                     GVADAPNPTALFQPDRIHPRSEAQPRMLDNVWPELRKLL"
     gene            844..1560
                     /locus_tag="PK518_07110"
     CDS             844..1560
                     /locus_tag="PK518_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48722.1"
                     /translation="MSETIAVPAQSLAATASQAPIIDVAHVHKSVTDSTGTLEILRDI
                     DFTLAERETVAIVGASGSGKSTLLSIIAGLDTPTRGTVRLAGEDLFALNEDERAALRA
                     RKLGFVFQSFQLLANLTALENVMLPLELAGHRDARRRAADMLGRVGLAQRLSHYPKVL
                     SGGEQQRVALARAFVVQPAVLLADEPTGSLDFATGETIMELMFALNREQGTTLVLVTH
                     DRAIAERCDRRITIEAGRLV"
     gene            complement(1585..2679)
                     /locus_tag="PK518_07115"
     CDS             complement(1585..2679)
                     /locus_tag="PK518_07115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM48723.1"
                     /translation="MPPPASAAQSDHRIDDKQRLRLRRLLLSVAVYLPCALMLLMVTW
                     LGFLPRWFTPLWLAIFTVTNTVFYALIKSGLNLRLNDPSMTMAQMTVAIAAVAVILYH
                     ADAIRGGMLMLLLVILLFGVLRLTTVELLAMGALSSAAYALVIVLLTTRKPQQIEPSV
                     EWTQWATLTATLAVMGPLVGYMSHVRRRLSESLRTIKDMAERDALTGAFNRHHMGEML
                     DREVSRCERGAPAFVLLIADIDHFKHINDSFGHLVGDLVLKRVAHAMREALRKGDYMA
                     RYGGEEFVLLIAANDLAESAVASERLRVQVERLRIPELGRHNITISIGGTVYRSGDTQ
                     STLLGRADAALYRAKHSGRNRVECDTPLAE"
     gene            complement(2809..4020)
                     /gene="sstT"
                     /locus_tag="PK518_07120"
     CDS             complement(2809..4020)
                     /gene="sstT"
                     /locus_tag="PK518_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HRM48724.1"
                     /translation="MLFSALLRWVQRTSLVTQIIIGLLVGLALAMLVPAATPSVAFLG
                     TLFISALKAIAPILVFVLVMAAIGNHRPGESTHMRPVLLLYLVGTLAAAIVGVTASLW
                     FPSTLVLQSAAQDQAAPGAVSQVLLNVVQSIVSNPVKALLEANYIGILAWAVALGIAL
                     RHASASTRTALQDGAQAVTLVIQWVIRCAPVGIMGLVAATFAEAGTQALWGYAHLLAV
                     LIGCMLFVALVVNPLIVFLVIRRNPYPLVFTCLRESGVTAFFTRSSAANIPINLALAK
                     RLNLNEETYSIAIPLGATINMAGAAITISVLSLAAAHTLGISVDMPTALLLCVVASVC
                     ACGASGVAGGSLLLIPLACSLFGISNDVAMQVVGVGFIIGVLQDSAETALNSSTDVLF
                     TATACMAQERG"
     gene            4174..4926
                     /gene="rlmB"
                     /locus_tag="PK518_07125"
     CDS             4174..4926
                     /gene="rlmB"
                     /locus_tag="PK518_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRM48725.1"
                     /translation="MSSPKVLFGFHAVGVRLKTAPQSIIEVYYEATRRDARMRQFLAR
                     AKEAGVRLIEADGLRLAKLAGSHGHQGVAARVEAVAQLQSLDDLLDQLEADGVTNPLL
                     LVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETVPYFMVT
                     NLARTLGELKERNIWVIGTSGDADKNLYQVDLKGPVALVLGAEGPGMRQLTRKTCDEL
                     VCIPMQGAVESLNVSVASAVCLYEALRQRGLA"
     gene            5041..5394
                     /locus_tag="PK518_07130"
     CDS             5041..5394
                     /locus_tag="PK518_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48726.1"
                     /translation="MIRMSAITVFVALVPGAFLLAPVCASAEGSVTIESPKDGARLDA
                     MKQTSLVYEVLPGPDGNHVHVYLDDKEVGILRQLKGSYALPTLAAGKHDICIKVVNKG
                     HTPIGLQRCVGVTVE"
     gene            5496..5807
                     /locus_tag="PK518_07135"
     CDS             5496..5807
                     /locus_tag="PK518_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48727.1"
                     /translation="MHPAGSRGLLWLALSGWALQLASGAVFGAVSWHFYGQFPDIHGI
                     AIGALVLKVLCALVALVLIALSLYARPLHDGRRSTVTWRLLATLGGTALTGAAFLRWF
                     S"
     gene            complement(5998..6462)
                     /locus_tag="PK518_07140"
     CDS             complement(5998..6462)
                     /locus_tag="PK518_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRM48728.1"
                     /translation="MSEFSSNDVFTLTTARILPARPEAVFSAIADPALLTRWWGPNGF
                     SSSFQTFDFRPGGDWVFTLHGPDGTDYPNVCRFIEIVADQRVVIEHLNMHWFELTLSL
                     APQDGGTRIGWQQRFDSPEACARMAPLCAPSNEQNLDRLASVLVLQPESIGT"
     gene            complement(6471..7142)
                     /locus_tag="PK518_07145"
     CDS             complement(6471..7142)
                     /locus_tag="PK518_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRM48729.1"
                     /translation="MTRVRVQAFTISLDGYAAGPDQDIDHPLGVGGTELHQWLLPTRT
                     LQRALFGKDGGTAGVDDEFAARGFRNVGAWILGRNMFGPIRGAWPDAGWKGWWGNNPP
                     YHVPVFILTHHARSSIAMEGGTNFHFITGGIREALDRAREAAAGMDVRIGGGPSTIRQ
                     YLREGLINELHLAIAPVLLGRGESLFQGLDLRTLGYECVEFVASQKAMHAVLRRQERA
                     GGPNE"
     gene            complement(7190..7582)
                     /locus_tag="PK518_07150"
     CDS             complement(7190..7582)
                     /locus_tag="PK518_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM48730.1"
                     /translation="MKPNPVVWFEIYVQDMERARKFYETVFRCQLEPMAAPEGEASGL
                     QMLGFPMTMEAPGAGGMLVQMADCPRPAGGPGTLVYFACTDCAVEQGRVEAAGGKVFK
                     PKFTIGPYGHCALVIDTEGNTIGLHSMQ"
     gene            complement(7588..8286)
                     /locus_tag="PK518_07155"
     CDS             complement(7588..8286)
                     /locus_tag="PK518_07155"
                     /inference="COORDINATES: protein motif:HMM:NF017771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HRM48731.1"
                     /translation="MEYPSRPPVVERGAWEGARSELLAREKAHTRAGDQLAAARRRLP
                     MTRMAPVTVTGANGPTPLREVFEGRHMLLVYHFMWKRGASHDKQCPGCTHTQVALNEA
                     VRAYLAERDVSYAVFSAGPWPEIAAYREFMGWTGPWYSTQDTGTALPATRDGGDLRCY
                     LRDGDDVFQTYATTARGVEAMLPTLQLLDLTAYGRQEAWEDSPAGWPQGEAGAWWWRD
                     GRPVAQWLRPPSTR"
     gene            complement(8301..8786)
                     /locus_tag="PK518_07160"
     CDS             complement(8301..8786)
                     /locus_tag="PK518_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM48732.1"
                     /translation="MMKPAKNTICLWYDGAAEEAARFYASTFPDSCVTALHRAPADYP
                     SGKQGDVLTVEFTVMGIPCLGLNGGPAFRHCEAFSFQVATADQAETDRYWNAIVGNGG
                     QESECGWCKDRWGISWQITPMVLIQGYTDPDRAAARRVFDAMMQMRKIDVAAIEAARR
                     G"
     gene            complement(8820..9266)
                     /locus_tag="PK518_07165"
                     /pseudo
     CDS             complement(8820..9266)
                     /locus_tag="PK518_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913164.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            complement(9295..9729)
                     /locus_tag="PK518_07170"
     CDS             complement(9295..9729)
                     /locus_tag="PK518_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM48733.1"
                     /translation="MQFNPYLNFDGNCAEAFAFYARLFGGTIVHQSSFGEMPPGEGMP
                     PIPESARSRIMHVHLQIKDQALMGSDAMPGADASCGGYQPAQGLWVSIQAADVAEGRR
                     LFDALAQGGQAAMPFAATFWSPGFGMVKDRFGTPWMVNVPQT"
     gene            complement(9767..10120)
                     /locus_tag="PK518_07175"
     CDS             complement(9767..10120)
                     /locus_tag="PK518_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRM48734.1"
                     /translation="MQSDHYVDGFLLAVPGAQKERYLQVASKAARLFKEHGALGVVEC
                     WGDDVPDGKLTSMPMAVRLKPGETVVFSWITWPSRQVRDTGMKAFMEDPRCPKDMPFD
                     GKRLIYGGFRVLLSA"
     gene            10204..10875
                     /locus_tag="PK518_07180"
     CDS             10204..10875
                     /locus_tag="PK518_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM48735.1"
                     /translation="MSDKKPSPTRSTFRHGDLRRALLDAGVALAREGGPGAIVLREAT
                     RRAGVVPNAAYRHFESRQALLEAVRAAALAALARAIEAEIDAVQQLPDPRRRARAAFR
                     GVGLGYLRFARREPGLFRTAFAARPFDVHERAAQDDAARGASGMDPFELLCHALDGLV
                     QAGVLPAAQRQGAEFMAWSAVHGMAMLLLDGPLRGLGEAPSQALAERLVAMVEQGLLA
                     EAAPQ"
     gene            complement(10890..11489)
                     /locus_tag="PK518_07185"
     CDS             complement(10890..11489)
                     /locus_tag="PK518_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM48736.1"
                     /translation="MTTALTLNLQPLDWLNLFLYFLSISLLAVGGAIATAPDMHRFLV
                     ERQGWLTELQFNTSIVLAQAAPGPNVLFIALLGWNLGLNAGGATPLAWAFGALGVALC
                     MLGVLLPSCVLTWAATRWAHRNRERRSVRAFKQGMAPLVIGLILATAWVLARAQGDAA
                     RDWPLWLLSAATTLLVWRTRIHLLWLLGAGALLGALGWV"
     gene            complement(11494..12075)
                     /locus_tag="PK518_07190"
     CDS             complement(11494..12075)
                     /locus_tag="PK518_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM48737.1"
                     /translation="MTAPTITPDQRPRPHSLGQLFWAFTWLALQGFGGVLAVVQRELV
                     ERRRWITNDEFIEDWAAAQILPGPNVVNLSIMVGDRYFGLRGALVAVAGMLLLPMLLL
                     LAVATLYAAFASHPMMAGALRGMGAVAAGLVAGMGCKLAASLARHPLGPLPCALITAA
                     TFIAMSVLRLPLAWVLPVVGGVACALTWRKLPA"
     gene            12236..12568
                     /locus_tag="PK518_07195"
     CDS             12236..12568
                     /locus_tag="PK518_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRM48738.1"
                     /translation="MHPYILLAISIAAEVVATSALKSSQGLTRLWPSVLAVLGYCAAF
                     YLVSKVMNHMATGVVYAIWSGLGIVLISLVGWWLYGQKLDLPAVVGMLMIVGGVVVIQ
                     VLSKTAGN"
     gene            12638..13972
                     /locus_tag="PK518_07200"
     CDS             12638..13972
                     /locus_tag="PK518_07200"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HRM48739.1"
                     /translation="MPSENHTLSATDAALATLLRRAEPCLWLNPRRQAVLPRAQPVAG
                     SSIGLDDTRAAAARFARFAPLLARLFPELQATAGVIESPLLPASGLHVGQGRLWLKAD
                     HLLPVAGSIKARGGMHEVLEFAERLALRHGLIGAQAGPDDYLALASPAARALFARHTV
                     AVGSTGNLGLSIGVAASALGFSAVVHMSLEAKEWKKERLRQRGVQVVEHAGDYEAAVA
                     AGRAQAAQDPFSHFVDDERSLSLLLGYSAAALHLQGQLRDAGIAVDAEHPLFVYLPCG
                     VGGAPAGITFGLRQVLGPHVHCFFAEPVQSPCFMLQMMAGQGAHPSVYDWGLSNQTEA
                     DGLAVPRASLPAAELMESLLSGCFTVHDDTLFRQLVQVLDATGESIEPSAAAGFGGPA
                     MLTGSDSGRAWLAEQGLQQHLARATHLVWTTGGLFVPPDEYERFVQRGRALS"
     gene            14022..15149
                     /locus_tag="PK518_07205"
     CDS             14022..15149
                     /locus_tag="PK518_07205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM48740.1"
                     /translation="MHHYDFVIIGAGIAGASMGWELAGQRRVLLLERESQPGYHTTGR
                     SAALYSATYGTANIRALTRASRAFYDAPPPEFGTSPILTPRGVVYLAGPDQLDLLDAA
                     YAEILPLNPGVRRLTRDELLAELPCLRPEAVAAGMSEPGAADIDVHALHQGYLRGLRQ
                     RGGAIRCNAEVTGLQRLDDGWRVTLAGGEAITAGAVVNAAGAWADVVAALAGVPPIGL
                     EPRRRTAFTFPVPAGMDASGWPAVIGIDESYYFKPDAGQLLGSPANADPVAPHDVVPE
                     ELDVALGIHRIEQVTSFQIRRPSHSWAGLRSFVADGDLVIGWDNHVPRFFWLAAQGGY
                     GIQSAAGAALLARQLALEEPLSASLVEEGVTRDALSPARLR"
     assembly_gap    15150..15249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15307..15897)
                     /locus_tag="PK518_07210"
     CDS             complement(15307..15897)
                     /locus_tag="PK518_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRM48741.1"
                     /translation="MPYTLHAPVHSELIIKKSRFIGCVQPMADRAGAQAVVDALWRQH
                     PGAAHVCWALLAGGQSAAVDDGEPGGTAGRPMLDVLRHQDLEGVLATVVRYFGGVKLG
                     AGGLVRAYTDSVAQALLQAEKVPLVRTRSLRCSVPYALEGLLRREIAAAGAPLLDVAH
                     AALVTLQWSLPEPEAEAFVARINDLCQGRAGWLDAQ"
     gene            16001..>17331
                     /locus_tag="PK518_07215"
     CDS             16001..>17331
                     /locus_tag="PK518_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRM48742.1"
                     /translation="MNALVDVSFFPRHAKPLTSYRPFWAKRFGPAAFLPMSREEMAQL
                     GWDSCDIVLVTGDAYVDHPSFGMAVIGRTLEAQGFRVGIISQPDWQSAEPFKALGKPN
                     LFWGVTAGNMDSMINRYTADRKIRTDDAYTPGAEGNRRPDRAAIVYSQRCREAYKDVP
                     IVLGGMEGSLRRIAHYDYWSDKVRRSIVVDSKCDLLLYGNAERALVEIAHRLARREPV
                     EKITDVRGTAFIRRPGDETAEGWMEIDSTEVDQPGKVEEHINPYQTTSEQAASQGASC
                     AKEDAAKIEAAGACSTGDAGQNASEKPLQFMPNPALVSKKALAREKTVIRLPSYEQVK
                     SDPVLYAHASRVFHLETNPGNARALVQAHGEGATARDVWLNPPPIPLTTAEMDHIFDL
                     PYARSPHPRYADEHGRHDGATKIPAWEVIRFSINIMRGCFGGCTFCSITEH"
BASE COUNT         2833 a         5675 c         5826 g         2897 t
ORIGIN      
        1 cggactttta atccgttggt cacaagttcg aatcttgtac gtcctaccaa gctatataag
       61 gaaagcgcac tatctaaaca atagtgcgtt ttttttgggt ttggcttgta gcttgtaggt
      121 gacctgtaaa cagtttgccg aaaaatggca tgtcccagcg cctggtccaa gctatagcag
      181 cttgcgcaac tcgggccaga cgttgtccag catacgcggc tgggcctcgg atcgcgggtg
      241 gatgcggtcg ggctggaaca gcgccgtggg gttgggtgca tcggccacgc ccttgagcaa
      301 gaagggcacc agggccgcct tgcgctcctt ggctacggcc tcaaacaggc tggcaaagcg
      361 gcgcccatag tcggcaccat agttgggtgg catctgcatg cccaccagca gcacgcgcgc
      421 gcctgccgcc tgcgcctgct cggtcatcca gtccaggttg tcctgggtat tcgccaacgg
      481 caggccgcgc aatgcgtcat tgcttccgag ctccagtacg acatgcgtgg gctgatgcag
      541 tttgagcagc gcggccaggc gtgcgcggcc accggctgtg gtgtcgccgc tgacgctggc
      601 gttaacgacc gtggccggga ttttttcctg tgccaggcgc tgttccagca gcgtgaccca
      661 acccgttccg cgtgccagac catactcggc gctgagcgag tcgccaacca ccaggatgac
      721 ggaacccttg gcggagccgt tcttgcctgg ctgggtgcca gcgtatgcca tgcccacaat
      781 ggccagggtg gccagcacga taaagtcacg cctcatcata gaaatcaact tcgagaacct
      841 ttcatgtccg aaaccattgc cgtccctgcc caaagcctgg cagcgaccgc gtcgcaggcg
      901 cccatcattg acgtagcgca tgtgcacaaa tccgtcaccg actctacggg cacgctggag
      961 attttgcgcg atatcgattt caccctggcc gagcgggaaa ccgtggccat cgtcggtgcc
     1021 tcgggttcgg gcaagagtac cttgctttcc atcattgccg ggctggatac acccacccgt
     1081 ggcacggtgc gcctggcggg ggaggacttg tttgcattga acgaagacga gcgtgccgcg
     1141 ctgcgtgcgc gcaaactggg gtttgttttc cagagcttcc agctgctggc caatctcacg
     1201 gcgctggaga acgtgatgct gccgctggag ctggcgggtc accgtgacgc gcgccggcgc
     1261 gccgccgaca tgctgggccg tgtggggctt gcgcagcggc tgtcgcatta ccccaaggtg
     1321 ctctccggcg gcgagcagca gcgtgtggcc ctggcgcgcg cctttgtggt gcagccagcc
     1381 gtgctgctgg cggacgaacc cacgggcagc ctggactttg cgaccggcga gaccatcatg
     1441 gaactgatgt tcgccctgaa ccgcgaacag ggcacgaccc tggtgctggt gacgcacgat
     1501 cgcgccatcg ccgagcgctg cgaccggcgc atcaccatcg aggccgggcg gctggtgtag
     1561 ccaagctgtg tcggtgcgcg accgtcactc ggccaggggt gtgtcgcatt cgacgcggtt
     1621 gcgcccgctg tgcttggccc ggtacagcgc cgcgtctgcc cgtccaagca gcgtggattg
     1681 ggtgtcgcca gaccggtaga cggttccacc tatcgatatg gtgatgttgt gcctacccaa
     1741 ttcaggaatg cgcaggcgct ctacctgcac gcgcaggcgc tcgctcgcaa ctgcggattc
     1801 agcaaggtcg ttggccgcaa ttagcagtac gaactcctcg cctccgtagc gggccatgta
     1861 gtcgcctttt cgcagcgcct cacgcatggc gtgggccacc ctcttcaaga ccaggtcgcc
     1921 caccagatgc ccaaaactgt cgttaatgtg cttgaagtga tcgatgtccg cgatcaacag
     1981 tacaaaggct ggggcaccgc gttcgcagcg gctgacctca cgatcaagca tttctcccat
     2041 gtgatggcga ttgaacgcgc cggtcagcgc gtcacgttcg gccatgtctt tgatcgtgcg
     2101 cagtgattct gacagcctgc gccgcacgtg gctcatgtaa cccaccagcg ggcccatcac
     2161 agccagggtg gcggtgagcg tggcccattg cgtccactcg accgatggtt cgatttgctg
     2221 cggcttacgc gtcgtgagca aaacgatgac cagcgcatag gcggccgacg atagtgcccc
     2281 catggctagc agttccacgg ttgttagccg cagcacaccg aacagaagaa tcaccagcag
     2341 cagcatcaac atgccgccac gaatggcgtc ggcgtgatag aggatcacgg ccactgcggc
     2401 aatggccacg gtcatttgcg ccatggtcat gctgggatca ttgaggcgca ggttcaaccc
     2461 gcttttgatc agcgcgtaga agacggtgtt cgtcaccgtg aaaatcgcca gccaaagcgg
     2521 agtgaaccag cgcggcaaaa aacccagcca cgtcaccatc agcagcatca acgcgcaggg
     2581 caggtacacg gccacggaaa gcaacaggcg ccgcaggcgt agcctttgct tatcatcgat
     2641 gcggtggtcg gactgcgccg ccgaagcggg gggtggcatg gggtttcggt accgatgatt
     2701 cctgatgtgt gtaattttgt atcttgccgg caaatttgca gtttggctac ggcatatcgg
     2761 cgtgagtagt attcaggcca caggggctgg cggctatatc gggacgtctc agccgcgttc
     2821 ctgcgccatg caggccgtgg cggtgaacag tacgtcggtg gaagagttca gcgccgtctc
     2881 ggcagaatcc tgcagcacgc cgatgatgaa acccacgccc accacctgca tggccacgtc
     2941 gttgctgatg ccaaacaggc tgcaggccag cggaatcaac agcagcgagc ccccggccac
     3001 gcccgaggcg ccgcaggcgc agaccgaggc caccacgcac agcaacagtg ccgtcggcat
     3061 gtccaccgag atgcccagtg tgtgggctgc ggccagcgac agcacgctga tggtgatggc
     3121 cgcgcccgcc atgttgatcg tcgcacccag gggaatggcg atgctgtagg tttcttcgtt
     3181 caggttcagg cgcttggcca gcgccaggtt gatggggatg ttggccgccg agctgcgcgt
     3241 gaagaaggcg gttacgccgc tctcgcgcag gcaggtgaag accagcggat agggattgcg
     3301 gcgaatgacc aagaatacga tcagcgggtt caccaccagc gccacgaaca gcatgcagcc
     3361 gatcagcacc gccagcaggt gagcgtagcc ccacagcgcc tgggtgccgg cctcggcaaa
     3421 ggtggcggct accaggccca tgatgccgac cggcgcgcag cgtatcaccc actggatgac
     3481 cagggtgacg gcctgcgcgc cgtcctgcag cgcggtgcgt gtgctggcgc tggcgtggcg
     3541 cagggcaatg cccagcgcca cggcccaagc gaggatgccg atgtagttgg cctccagcag
     3601 cgccttgacc gggttgctga cgatgctttg caccacgttc agcaatacct gagacaccgc
     3661 acccggcgcg gcttgatcct gcgccgccga ttgcagcacc agcgtgctcg ggaaccacag
     3721 gctggcggtc acgccgacga tggcagcggc cagggtgcca acgaggtata gcagcagcac
     3781 ggggcgcatg tgcgtgctct ctcccgggcg gtggttgccg atcgcggcca tcaccagcac
     3841 gaacaccagt attggcgcaa tggctttcag ggcggagatg aacaaggtgc ccagaaaggc
     3901 caccgagggc gtggccgctg gtaccagcat ggccagggcc aggccgacca gcaggccgat
     3961 gatgatctgg gtgaccaggc tggtgcgctg cacccagcgc agcaaggcgg aaaacaacat
     4021 gatgggatac acgtgcaaag cgcgcgccag gcgcacggat cggtgcagcg ggcatttcca
     4081 ccaaggcggg ttgccttggc gtgcgcgggg ttgtatggag gggaacccgg tattttaaaa
     4141 gctgcaccgg cgcatcgctg gataatcccc tccatgtcgt cccccaaagt cctgttcggc
     4201 tttcatgccg tgggcgtgcg cctgaagacg gcgccgcaat ccatcatcga ggtgtattac
     4261 gaggccacgc gccgtgatgc gcgcatgcgc cagttcctcg cgcgtgccaa ggaggccggc
     4321 gtgcgcctga tcgaggccga cggcctgcgc ctggcgaagc tggccggcag ccatggccac
     4381 cagggcgtgg ccgcgcgcgt ggaggccgtg gcccagctgc agtcgctgga cgatctgctt
     4441 gaccagctgg aggccgatgg cgtgacgaat ccgctgctgc tggtgctcga cggcgtgacc
     4501 gacccgcaca acctgggcgc ctgcctgcgc gtggccgatg gcgccggcgc gcatgcggtg
     4561 atcgcgccca aggatcacgc ggtgggcatc aacgccacag tggccaaggt ggccagcggc
     4621 gcggccgaga ccgtgcccta cttcatggtg accaacctgg cgcgcaccct cggcgagctc
     4681 aaggagcgca atatctgggt gataggcacc agtggcgacg ccgacaagaa cctctaccag
     4741 gtggacttga agggccccgt ggccctggtg ctgggcgccg agggccccgg catgcgccag
     4801 ctcacgcgca agacctgcga cgagctggtg tgcatcccca tgcagggcgc cgtggagagc
     4861 ctgaacgtct ctgtggccag cgctgtctgc ctgtacgaag cgctgcgcca gcgcggcctg
     4921 gcatagcggc tggctcgaat ccgcggtgcc atcaggggtg acgcatgtgc gtcggcaacc
     4981 ccgcacaatc gcagcgtatg cttgcggcgt cagctgtatc catcactgat tggagacgtc
     5041 atgatccgca tgtcagccat cacggttttt gttgccttgg tgccgggggc attccttttg
     5101 gcacctgtct gcgcctcggc cgagggctcg gtcacgatcg agtctcccaa ggacggtgcg
     5161 cgactcgacg cgatgaagca aaccagtctc gtgtacgagg tgctgcccgg gcccgacggc
     5221 aaccatgtgc atgtctactt ggacgacaag gaggtcggca tcctgcgtca gctcaagggt
     5281 agttatgccc tcccgacgct ggccgccgga aagcacgaca tctgcatcaa ggtcgtcaac
     5341 aaggggcata cgccgatcgg cttgcagcgc tgcgtcggcg tcaccgtcga gtaatcgatg
     5401 tggatctgca gaaccttgcg tatgcgctga cccaggtcgc ccataacttc ggggccgtgg
     5461 cggtcgttgc agttcctctg tatgcgcttt gttccatgca ccccgcgggc tcgcgcggcc
     5521 tgctgtggct ggcgttgtcg ggctgggcgc tgcagctcgc cagcggtgct gtgtttggcg
     5581 cggtgagctg gcatttctac gggcaatttc cggacatcca cggcattgcg attggtgccc
     5641 tggtgctcaa ggtgttgtgc gcgctggtgg cgctggtact gatcgcgctg tcgctgtacg
     5701 cacgtccttt gcacgatggc cgtcgcagca ccgtgacctg gcgcctgctg gccacgctgg
     5761 gcggtacggc actgacgggc gcggcgtttt tgcgctggtt ttcgtgatcg caatgcgacg
     5821 gtgtggccgg gtgttcaacg cccagggttt cttgccgctt gggctagtac tgcaactcgg
     5881 aagtagagga acacaatgaa atcgatgcat tcgcgcgcaa ggcaaggcgc aaaccgcagc
     5941 aatagccgta gccattgcga ggatttgcaa cgccgccatg cgggcgaagg cgcgatttca
     6001 cgttccgata ctttcgggtt gcagtactag tactgaggcc aatcgatcca ggttctgctc
     6061 gttgctggga gcgcacagcg gggccatgcg cgcgcaggcc tcggggctgt cgaagcgctg
     6121 ctgccagccg atgcgggtgc cgccgtcctg cggtgccagg ctgagcgtca gctcgaacca
     6181 gtgcatgttc aggtgttcga tcaccacccg ctgatcggcc acgatctcaa taaagcggca
     6241 gacgttgggg tagtcggtgc catcggggcc gtgcagcgtg aagacccagt cgccaccggg
     6301 gcggaagtca aaggtctgga agctgctgct aaagccattg gggccccacc agcgtgtcag
     6361 tagcgccggg tcggcgatgg cgctaaaaac ggcctcgggc cgggcgggca ggatacgcgc
     6421 cgtggtcagc gtgaagacat cgttggatga aaattcgctc atggcttggc tcactcgttt
     6481 gggccgccgg cgcgctcttg gcgccgcagg acggcatgca tggctttctg cgatgcgaca
     6541 aattcaacgc attcgtatcc cagggttcgc aggtcaagcc cctgaaacag cgactcgccc
     6601 cggcccagca ggacgggcgc gatggcaaga tgcaactcgt tgatgaggcc ctcgcgtaga
     6661 tactgccgga tggtgctcgg cccgccgcct atgcgcacgt ccattcccgc cgcggcctcg
     6721 cgcgcacggt cgagcgcctc gcggatgcct ccggtgatga agtgaaagtt cgtgccgcct
     6781 tccatcgcta tggaggaacg cgcatgatgg gtcaggatga agaccggaac gtggtacggc
     6841 ggattgttgc cccaccagcc tttccagcct gcgtccggcc atgcgccgcg aatcgggccg
     6901 aacatgtttc tgccaagaat ccaggcgccc acattccgaa aaccacgggc ggcgaactca
     6961 tcgtccactc cggccgtgcc gccgtccttg ccgaacaagg cccgttggag ggtacgcgtt
     7021 ggcagtagcc actggtgcag ttccgtcccg cccacgccga gcggatggtc gatgtcctga
     7081 tccgggcccg ctgcgtatcc gtcaagagat atggtgaagg cctgaacgcg aactcttgtc
     7141 attgattgcc tccgctggac gcccgaacgg ttgctgcgcc ctcgccggct tactgcatcg
     7201 aatgcaggcc gatggtgttg ccctcggtgt cgatcaccag cgcgcagtgg ccgtatgggc
     7261 cgatggtgaa ctttggtttg aacaccttac cgcctgccgc ctcgacgcgg ccctgctcga
     7321 cggcgcagtc cgtgcatgcg aagtacacca gggtgcctgg cccacccgcg ggccgcgggc
     7381 agtcggccat ctgcaccagc atgccgccgg cgccgggcgc ctccatggtc atgggaaagc
     7441 cgagcatctg caggccgctg gcttcgccct cgggggcggc catgggctcc agttggcagc
     7501 ggaacacggt ttcgtagaac ttgcgtgcgc gctccatgtc ttgcacgtag atctcgaacc
     7561 agacgacagg attgggtttc atgctgttca ccttgttgaa ggggggcgca accactgggc
     7621 cacgggccgg ccatcgcgcc accaccaggc tccggcctcg ccctgtggcc agccggcggg
     7681 ggagtcttcc caggcttcct gccgcccgta ggccgtgagg tcgagcagtt gcagcgtggg
     7741 tagcatggcc tcgaccccgc gtgcggtggt cgcgtaggtc tggaacacat catcgccgtc
     7801 gcgcaggtag cagcgcaggt cgccgccgtc gcgggtcgcc ggcagcgcgg tgccggtgtc
     7861 ttgtgtcgaa taccagggac cggtccagcc catgaattcg cgataggcgg cgatctcggg
     7921 ccaagggccg gcgctgaaga ccgcgtaact cacgtcgcgt tcggccaggt aagcgcgcac
     7981 ggcctcgttc agcgccacct gggtgtgcgt gcagccgggg cattgcttgt catgtgacgc
     8041 gccgcgtttc cacatgaagt ggtagacgag cagcatgtgc cggccctcga acacctcgcg
     8101 cagcggcgta ggcccgttgg cgccggtgac cgtgacgggc gccatgcgcg tcatcggcag
     8161 gcgccggcgt gccgcggcca gttggtcgcc ggcccgggta tgcgccttct cgcgggccag
     8221 caactccgat cgtgcgcctt cccacgcgcc acgttcgacg acgggaggcc tgctggggta
     8281 ttccatggtc atgctccttt tcaaccgcgc cgcgccgcct cgatggccgc aacgtcgatc
     8341 ttgcgcatct gcatcatggc gtcgaacacg cgcctggcgg cggcgcggtc tgggtcggtg
     8401 tatccctgga tcagaaccat cggcgtgatc tgccaggaga tgccccacct gtccttgcac
     8461 cagccgcact cgctctcctg gccgccgttg ccgacgatcg cgttccagta gcggtcggtc
     8521 tcggcctggt ctgccgtcgc cacctggaac gagaaggcct cgcagtgcct gaaggccggc
     8581 ccgccgttga gccccaggca ggggatgccc atgaccgtga actccacggt cagcacgtcc
     8641 ccctgcttgc ccgaggggta gtcggccggc gcgcggtgca gcgcggtgac gcacgagtcg
     8701 ggaaaggtgc tggcgtaaaa gcgcgccgct tcttcggcgg cgccgtcgta ccaaaggcag
     8761 atggtgttct ttgcgggctt catcatgttt gcgtccatgc cccttcgggc cagcctgttc
     8821 tagcctggaa tctccggctc ggcgagcgcc gccagctgtg ccagcgactc ctgccagctc
     8881 aggtagcaca tctccagcgg gatggcctca gggatgccgg cctaagtgat gcgtagatcc
     8941 gtgccgcatg gcacgggttg gagccgcacc gtcacctcca gcaagccggg caggctggga
     9001 tcgtcaaact ggtcggtgta gcggatgagt tcacccggca ccagctccag gtattcgccg
     9061 tgaaaggcgt gtgcgccgcc gctgccgaag ttgtggaacg acatgcgtaa gctcccgccc
     9121 acgcgcgcct ccaggtgatg cacctggcag gtaaagccgt gtggcggcag ccacttggcc
     9181 atggcgccgg cctcggtgaa ggcgcggtac agcctttgcg gcgggcaacg cagtacgcga
     9241 tgcagatgga tggttcgctc ggacatgtga gcctccgggg tagaaacagc gggatcaggt
     9301 ctgcgggaca ttcaccatcc acggcgtgcc gaagcgatcc ttgaccatgc caaagcccgg
     9361 cgaccagaag gtggcggcaa acggcatcgc cgcctggccg ccctgggcca gggcatcgaa
     9421 caggcgccgg ccctcggcca cgtcggcggc ctgaatcgac acccacaggc cctgcgccgg
     9481 ctggtagccg ccgcagctgg cgtcggcgcc gggcatggcg tcggagccca tcagcgcctg
     9541 atccttgatc tgcaggtgga catgcatgat gcggctcctg gccgactcgg ggatgggcgg
     9601 catgccctcg ccaggcggca tctcgccgaa gctgctctgg tgcacgatgg tgccgccaaa
     9661 cagcctggcg tagaaggcaa acgcctcggc gcaattgccg tcgaaattca ggtagggatt
     9721 gaattgcatg gatgcctcct gtggttgaaa cccgggcgcg gcgacgctat gcgctcagca
     9781 gcacgcgaaa gccgccgtag atcaggcgct tgccgtcgaa cggcatgtcc ttggggcagc
     9841 gcgggtcttc catgaaggcc ttcatgcccg tgtcgcgcac ctggcgcgag ggccaggtga
     9901 tccacgagaa caccacggtc tcgcccggct tcagcctgac ggccatcggc atcgaggtca
     9961 gcttgccatc gggcacgtcg tcgccccagc attcgacgac ccccagggcg ccatgctcct
    10021 tgaacagccg cgccgctttg ctggcgacct gcagatagcg ctccttctgc gcgccgggaa
    10081 cggcaagcag aaagccatcg acatagtggt cggattgcat ggcgcgcctc ctttgtggac
    10141 ggcgtacact tatacgcctg tccaagctga aattgaccga ggtctaggta agcctgagta
    10201 aaaatgtcgg ataaaaaacc aagccccacg cgcagcacct ttcgccatgg cgacctgcgc
    10261 cgtgccctgc tggacgcggg cgtggcgctg gcgcgtgagg gcgggcctgg cgccatcgtg
    10321 ctgcgcgagg ccacgcgccg cgccggcgtg gtgcccaatg ccgcatatcg gcatttcgag
    10381 agccgccagg ccctgctgga ggccgtgcgc gccgccgcgc tggccgcact ggcgcgcgcc
    10441 atcgaggccg agatcgacgc cgtccagcag ctgcccgacc cgcgccggcg cgcgcgcgcc
    10501 gccttccgtg gcgtgggcct gggttatctg cgctttgcgc ggcgggagcc ggggctgttt
    10561 cgcaccgcct tcgcggcgcg gccctttgac gtgcatgagc gcgcggcgca ggacgacgcg
    10621 gcgcgcggcg ccagcggcat ggatccgttc gagctgctgt gccacgcgct ggacggcctg
    10681 gtgcaggccg gcgtgctgcc ggccgcgcag cgccagggcg ccgagttcat ggcctggtcg
    10741 gccgtgcacg gcatggcgat gctgctgctc gacggcccct tgcgcggcct gggcgaggcc
    10801 cccagccagg cgctggcgga gcgcctggtg gcgatggtgg agcagggctt gctggccgag
    10861 gccgcgccgc aatgaggcgc cgcgcggggt cagacccagc ccagcgcgcc cagtagcgcc
    10921 cccgcgccca gcagccacag cagatggatg cgcgtgcgcc acaccagcag cgtggtggcg
    10981 gccgacagta gccacagcgg ccagtcgcgc gccgcatcgc cctgggcgcg ggccagcacc
    11041 caggcggtgg ccagtatcag cccgatcacc agcggcgcca tgccttgctt gaaggcacgc
    11101 accgagcggc gctcgcggtt gcggtgcgcc cagcgcgtgg cggcccaggt cagcacgcag
    11161 ctgggcagca gcacgcccag catgcacagc gccacgccca gcgcgccaaa ggcccaggcc
    11221 agcggcgtgg cgccgcccgc gttcagtccc aggttccagc ccagcagggc gatgaacagc
    11281 acgttcgggc cgggggcggc ctgggccagc acgatggagg tgttgaactg cagctcggtg
    11341 agccagccct ggcgctccac cagaaagcgg tgcatgtcgg gcgcagtggc aatggcgccg
    11401 cccacggcca gcagcgagat cgacaggaag tacagaaaca ggttcagcca gtccagcggc
    11461 tgcaggttca gcgtcagtgc ggtggtcatg cggtcacgcg gggagcttgc gccaggtgag
    11521 ggcgcaggcc acgccgccga ccacgggcag cacccaggcc agaggcaggc gcagcacgct
    11581 catggcgatg aaggtggcgg cggtgatcag cgcgcagggc aggggcccca gggggtgccg
    11641 ggccagcgat gccgccagct tgcagcccat gcctgccacc agcccggcgg ccacggcgcc
    11701 catgccgcgc agcgcgccgg ccatcatggg gtggctggca aaggccgcgt acagcgtggc
    11761 cacggccagc agcagcagca tgggcagcag caacatgccg gccacggcca ccagggcgcc
    11821 gcgcaggccg aagtagcggt cgcccaccat gatggacagg ttcaccacgt tcgggccggg
    11881 caggatctgg gcggcggccc agtcctcgat aaattcgtcg ttcgtgatcc agcgccggcg
    11941 ctccaccagt tcgcgctgca ccacggccag cacgccacca aagccctgca gcgccagcca
    12001 ggtgaaggcc cagaacagct ggcccaggct gtgcgggcgc ggacgttgat cgggtgtaat
    12061 ggtgggggcg gtcatggtta cgggcatggc gctggctgcg aagccggcca tggtaagccg
    12121 gccgcgccat gcctgtcgta catgagtcaa aataacctgt aacgcccaat agataagcgc
    12181 tgggagctat cattttcatg ttttcaccat gggtggcgag gcaactgttc catcaatgca
    12241 cccctatatc ctgctcgcca tctccatagc ggccgaggtc gtcgcgacct cggccctcaa
    12301 atcctcccag ggtcttaccc gcctgtggcc cagcgtgctg gccgtgctgg gctattgcgc
    12361 ggccttctat ctggtgtcca aggtcatgaa ccatatggcc accggcgtgg tgtacgccat
    12421 ctggtcgggg ctgggcatcg tgctgatctc gctcgtgggc tggtggctgt atggccagaa
    12481 actggacttg ccggccgtcg tcggcatgct catgatcgtg ggcggtgtgg tggtgataca
    12541 ggtgctgtcc aagacggctg gaaattaggc attgaacagg catctggcgc ttatccagca
    12601 agcgtcaaaa gctataaatt tgaaagcaat tatggccatg ccgtccgaga atcacaccct
    12661 gtccgccacg gatgcggccc tcgcgacctt gctgcgccgg gccgaaccct gtttgtggct
    12721 caacccccgg cggcaggcgg tgcttccccg ggcgcagccg gtggccggca gctccatcgg
    12781 cctggacgat acgcgcgccg ccgcggcgcg gtttgcgcgt ttcgcgccgc tgctggcgcg
    12841 cctgttcccc gagctgcagg ccacggcggg cgtgatcgag tcgccgctgt tgccggcgtc
    12901 cggcctgcat gtcgggcagg ggcgtttgtg gctcaaggcc gaccatttgc tgcccgtggc
    12961 cggctccatc aaggcgcgcg gcggcatgca tgaggtgctg gagtttgccg agcgcctggc
    13021 cctgcggcat ggactgatag gcgcgcaggc cgggcctgac gactatctgg ccctggccag
    13081 tcctgcggcg cgcgccctgt ttgcgcgcca tacggtggcc gtgggctcca ccggcaacct
    13141 gggcctgtcc ataggcgtgg ccgcgtcggc cctgggcttc tcggccgtgg tgcatatgtc
    13201 gctggaggcc aaggaatgga agaaggagcg cctgcgccag cgcggcgtac aggtggtgga
    13261 gcatgccggc gactacgagg cggcggtggc ggccggccgc gcccaggctg cgcaagaccc
    13321 gttcagtcat ttcgtcgatg acgagcgttc gctgtccctg ctgctgggct acagcgccgc
    13381 ggcgctgcac ctgcaggggc agctgcggga cgcgggcatt gccgtggatg ccgagcaccc
    13441 gctgttcgtc tatctgccct gcggcgtggg cggcgcgccg gcgggcatca cctttggcct
    13501 gcgccaggtg ctggggccgc atgtgcattg cttctttgcc gagccggtgc agtcgccctg
    13561 cttcatgctg cagatgatgg ccgggcaggg cgcgcatccc tcggtgtacg actggggcct
    13621 ctccaaccag accgaggccg acggcctggc cgttccgcgt gcctcgctgc cggcggccga
    13681 gctgatggag tcgttgctct cgggctgctt cacggtgcat gacgacacgc tgtttcgtca
    13741 gctggtgcag gtgctggacg ccacgggcga gagcatcgag ccctcggccg ctgccggctt
    13801 tggcgggccg gccatgctga cgggcagcga ctcgggtcgc gcctggctgg ctgagcaggg
    13861 cctgcagcag catctggccc gggccacgca cctggtgtgg acgaccggcg ggctcttcgt
    13921 gccgccggac gaatatgagc gcttcgtcca gcggggacgg gcgctgtcct gaacaactgc
    13981 ccatggccac agccaacgct tcgttactga accctgaacc catgcaccac tacgacttcg
    14041 tcatcatcgg cgcgggcatt gccggcgcct ccatgggctg ggaactggcc gggcagcgcc
    14101 gcgtgctgct gctggagcgc gaatcccagc ccggctacca caccacgggg cgctcggcgg
    14161 cgctgtactc ggctacctat ggcacggcca atatccgggc gctgacgcgc gccagccgcg
    14221 ccttctatga cgcgccgccg cccgagtttg gcacctcacc catcctcacg ccgcgcggcg
    14281 tggtctatct ggcggggccg gatcagctcg atttgctgga cgcggcctat gccgagatcc
    14341 tgccgctcaa ccccggcgtg cgccgcctga cgcgcgatga gctgctggcc gagctgccct
    14401 gcctgcgccc cgaggccgtg gccgccggca tgagcgagcc cggcgccgcc gacatcgacg
    14461 tgcacgcgct gcaccagggc tatctgcgcg gcctgcgcca gcgcggcggc gccatccgct
    14521 gcaatgccga ggtgacgggg ctgcagcgcc tggatgacgg ctggcgcgtg acgctggccg
    14581 gtggcgaggc catcaccgcc ggcgccgtgg tgaatgccgc cggtgcctgg gccgacgtgg
    14641 tcgcagcact ggccggcgtg ccgccgattg gcctggagcc gcgccggcgc acggccttca
    14701 ccttccccgt gcccgcaggc atggacgcca gcggctggcc ggccgtcatc ggcatagacg
    14761 agagctacta cttcaagccc gacgcgggcc agctgctggg ctcgccggcc aacgccgacc
    14821 cggtggcgcc gcacgacgtg gtacccgagg agctggacgt ggcgctgggc atacaccgca
    14881 ttgagcaggt caccagcttt cagatccggc gcccgtcgca cagctgggcc gggctgcgct
    14941 ccttcgtggc cgatggcgac ctggtgatcg gctgggacaa ccatgtgccg cgcttcttct
    15001 ggctcgccgc ccagggcggc tacggcatac agagcgcggc cggcgcggcg ttgctggcgc
    15061 ggcagcttgc gctggaagag ccgctttcgg ccagcctggt ggaggagggc gtgacgcgcg
    15121 atgcgctgtc gcccgcgcgc ctgcgctgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnng gcatgcagaa taaaacgatg ccttgcctat cggccgaatg gcccgactct
    15301 tgagccctat tgcgcgtcca gccagccggc gcgcccctgg cacaggtcgt tgatgcgcgc
    15361 cacaaaggct tcggcctcgg gctcgggcag gctccattgc agcgtgacca gggccgcatg
    15421 ggccacgtcc agcagaggcg cgccggcggc cgctatctcg cggcgcagca ggccctcgag
    15481 cgcatagggc acgctgcagc gcaggctgcg cgtgcgcacc aggggcactt tctcggcctg
    15541 cagcagggcc tgggccacgc tgtcggtata ggcgcgcacc aggccgcccg cgcccagctt
    15601 cacgccgcca aagtagcgca ccacggtggc cagcacgccc tccagatcct ggtggcgcag
    15661 cacgtccagc atgggccggc cggccgtgcc gccgggctcg ccgtcgtcca ccgcggccga
    15721 ctggccgccg gccagcagcg cccagcagac atgggccgcg cccgggtgct ggcgccacag
    15781 cgcgtccacc accgcctggg cgccggcccg gtccgccatg ggctgcacgc agcctatgaa
    15841 gcggctcttc ttgatgatga gctcgctgtg gaccggggcg tgcagggtat agggcatggg
    15901 gcggcgagct taccccatgg ccctgatcgc caaaggccca cgctgggcgc cgctatcggc
    15961 aaacttttaa aatatcggcc cccaccccgc aggcccgccg atgaacgccc ttgttgatgt
    16021 ctcctttttt ccgcgccatg ccaagcccct gacgagctac cgcccgttct gggccaagcg
    16081 ttttggcccg gcggcgtttt tgcccatgag ccgcgaagaa atggcgcagc tgggctggga
    16141 cagctgcgac atcgtgctgg tgacgggcga cgcctatgtc gatcacccga gctttggcat
    16201 ggcggtgatc ggccgcacgc tggaggccca gggctttcgc gtgggcatca tctcccagcc
    16261 cgactggcaa agcgccgagc ccttcaaggc cctgggcaag ccgaatctgt tctggggcgt
    16321 gacggcgggc aacatggatt ccatgatcaa ccgctacacg gcggaccgca agatccgcac
    16381 cgacgacgcc tacacccccg gcgccgaggg caaccgccgc ccggaccgcg ccgccatcgt
    16441 ctacagccag cgctgccgcg aggcgtacaa ggacgtgccc atcgtgctcg gcggcatgga
    16501 agggtcgttg cgccgcatcg cccactacga ctactggagc gacaaggtgc gccgctccat
    16561 cgtggtcgat agcaaatgcg acctgctgct ctacggcaac gccgagcgcg ccctggtcga
    16621 gatcgcccac cgcctggcgc ggcgcgagcc ggtggagaag attaccgacg tgcgcggcac
    16681 ggccttcatc cgccgcccgg gcgacgaaac ggccgagggc tggatggaga ttgactcgac
    16741 cgaggtggat cagcccggca aggtggagga gcacatcaac ccctaccaga ccaccagcga
    16801 gcaggctgcc agccagggcg cgagctgcgc caaggaagat gctgcaaaaa tagaagctgc
    16861 tggcgcttgc tccacgggcg atgcaggcca aaatgcatct gaaaaaccgc tgcagttcat
    16921 gcccaatccg gcgctggtga gcaaaaaggc cctggcgcgt gagaagaccg tgatccgcct
    16981 gcccagctac gagcaggtga aaagcgaccc cgtgctctac gcccacgcta gccgggtgtt
    17041 ccacctggag accaaccccg gcaacgcccg cgccctggtg caggcgcacg gcgagggggc
    17101 cacggcgcgt gacgtgtggc tcaacccgcc gcccataccg ctgaccacgg cggagatgga
    17161 ccacatcttc gatctgccct acgcccgcag cccgcacccg cgctacgccg acgaacatgg
    17221 ccgccacgac ggcgcgacca agatcccggc ctgggaggtg atccgctttt ccatcaacat
    17281 catgcgcggc tgctttggcg gctgcacctt ctgctccatc accgagcacg a
//
LOCUS       DAOMTQ010000054        17116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_478_length_17116_cov_25.837348, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000054 DAOMTQ010000000
VERSION     DAOMTQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 17116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17116
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_17116_cov_25.837348"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2402)
                     /locus_tag="PK518_07220"
     CDS             complement(<1..2402)
                     /locus_tag="PK518_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRM48743.1"
                     /translation="MIAKLIRWSVANRFLVLLATAMLTAWGVWGVRSTPVDALPDLSD
                     VQVIIRTSYPGQAPQIVENQVTYPLATTMLSVPGAKTVRGFSFFGDSFVYVLFEDGTD
                     LYWARSRVLEYLNQVQGRLPATAKPALGPDATGVGWIFQYALVDRTGKNDLAQLRALQ
                     DWFLKFELKSLPNVAEVASVGGMVKQYQVVLDPVKLASYGLSQEQVRAALVAANQETG
                     GSVLELAGAEYMVRASGYLKTLDDFRAVPLVARGGVPVRLGDVATLQIGPEMRRGIAE
                     LDGEGEVAGGVVILRSGKNAQETIAAVKAKLGELQASLPQGVEIVTTYDRSALIERAI
                     RNLTIKLGEEFLVVALVCVLFLWHLRSALVAIISLPLGVMMAFLIMRYQGINANIMSL
                     GGIAIAIGAMVDAAVVMIENAHKKLEAWQHAHPDQRLQGKERWEVITRAAEEVGPALF
                     FSLLIITLSFIPVFTLEAQEGRLFGPLAFTKTYAMAAAAGLSVTLIPVLMGYWIRGRI
                     PDEQKNPITRMLIAVYRPSLEWVLRWPKATLLIAVLALATTAWPLARLGGEFLPRLDE
                     GDLLYMPSALPGLSAQRATELLQLSNRMIKTVPEVETVFGKAGRAETATDPAPLEMFE
                     TSVKLKPREQWRAGMTPEKLIEELDQAVKIPGLSNIWIPPIRNRIDMLATGIKSPIGV
                     KVTGNDLHVIDRIAAEVEQVAKGIPGVSSSLAERLTGGRYVDVQIDRVAAGRYGLNVA
                     DVQAVVAGAVGGENVSETVEGLARFPINLRYAREWRDSPERLKQLPIFTPMGQQITLG
                     TVA"
     gene            complement(2399..3880)
                     /locus_tag="PK518_07225"
     CDS             complement(2399..3880)
                     /locus_tag="PK518_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM48744.1"
                     /translation="MNNSLKKSLAGLTLIAIGVAAGWGLSQWRSSTHAVDGAAPQAGA
                     AAERKVLYWYDPMSPTQKFDKPGKSPFMDMDLVPKYADEDAQDSVGLSVSAQTVQALG
                     LRTAEVVQRAIGSDVDAVGTVLLNDRDVSIVQARSAGFVERVYARAPGDVIAAGAPLA
                     DLLLPEWVAAQREFLAVRALKDESLTAAARQRLLLLGMPQALVAQVERTGEPRGVYTV
                     TTPQGGLVAELMVRQGMTISAGASLARVNGLGTVWIEAAVPEAQSGPLQLGQEAQVRL
                     AAFPGEVLRARIVSILPQANSDTRTVRVRLELANPGQRLKAGMSGQITLKGREQPALL
                     VPSEAVIRTGKRALAYVVDGPGKFHPVEVQLGAEIGDQLVVQGGLEAGQQVVASAQFL
                     IDSEASLRGLLPAAPGATPEHGAHGAAPAYTVRGVVEEVSTAELTLAHDAIPALKWPA
                     MTMGFKLADPRLAAGLAPKQQVRFTFSKQGEDYVITAIEGIKP"
     gene            complement(3895..5082)
                     /locus_tag="PK518_07230"
     CDS             complement(3895..5082)
                     /locus_tag="PK518_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRM48745.1"
                     /translation="MFAASRTYALSFAEAREIAEQQSPRVSAQRLQIDAVESAQKAAG
                     TLPDPKLSVGLENLPISGMDRWSLTRESMTGQRLALMQEVPNQAKRAAKVASAQARVE
                     RERAALVLQRLQIRQELGLAWIAAQAVEQREQLLTELLAENQRLQDSLPARVAGGSAQ
                     AGDLLAAQQEALALSDRRDDLQRDRSKARAMLRRWVGPRADESLQGGTGPLIHPVAQL
                     RAELSSHAELALYPTMQSMARAESHEAQSESRGDWSWEIAYSRRDRRWGDMVSFQVTF
                     DLPWQKDRRQTPMIQAKQRELERLEAEQEDVARRHLQELDDSAAELQALDSQIERLKS
                     TGLQLAQGRAELALGNYRAAKGDLGAVLGARAQVLETRLRLIDLQAQRDGVTTRLNSL
                     IAD"
     gene            complement(5287..5823)
                     /locus_tag="PK518_07235"
     CDS             complement(5287..5823)
                     /locus_tag="PK518_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48746.1"
                     /translation="MVALLSWDGSLIRGKLSHIQHSEHPTMVSIRRIVTQWGRRIVGG
                     LIVTLLFSQMALAMYACPKLDADTHAASMQMAGMAMDEAAGMSAMPDCHAMPGTMDDE
                     SPQLCRAHCSGDGKPAPSPQGLDVPSIAAAHAVWIAYLLPVVPDPQSSTGARFDPAWL
                     DHRTGSLPLYLTFQVLRN"
     gene            complement(5868..6128)
                     /locus_tag="PK518_07240"
     CDS             complement(5868..6128)
                     /locus_tag="PK518_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48747.1"
                     /translation="MMNGMYMNDMAWRFGAHWLMMLLGAVVIVLPFWKIFAKAGFSGW
                     LGLLMIVPMVNLIALYVLAFSEWPRSKRADKLDSGEMNPGSR"
     gene            complement(6164..8275)
                     /locus_tag="PK518_07245"
     CDS             complement(6164..8275)
                     /locus_tag="PK518_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HRM48748.1"
                     /translation="MEAPAGTIYTCPMHPEVQQDHPGNCPKCGMSLEPVIPLDEEDNS
                     ELIDFQRRFWWTLPLTVVVTILAMFGHRLGWFDMKTQTWVELVLSLPVVLWTGWPFFV
                     RCWQSIVNRSPNMWTLIGLGTGAAFVYSVVATLAPGVFPDSFIAHGRVAVYFEAAVVI
                     ISLTMLGQIIELKARSQTSAAIKALLGLAPKTARRINADGSEEDVPLNHVHVGDLLRV
                     RPGEKVPVDGVVTEGSSAVDESMLTGEPVPVTKRAGDGLIGATMNTSGALVMRSEKVG
                     AATMLSQIVQMVANAQRSKAPMQRMADVVAGKFVVVVVLIAIATFFVWGFFGPEPSWV
                     FGLINAVAVLIIACPCALGLATPMSVMVATGRAATQGILFRDAGAIEKMREVDTLIVD
                     KTGTLTEGKPAFDTAVADPGYTPDEVLRLAASLDQGSEHPLADAIVRAARDKGLSLTK
                     ADDFESGSGIGVRGTVEGKHLVLGNTALMQQENVATDALKADAERLRSEGGSVMHLAV
                     DGRLAGILAVTDPIKATTADAIKTLHDSGLRIVMATGDGLTTARAVGAKLRIDEVHGE
                     VKPADKLALVERLQKEGHVVAMAGDGINDAPALAKADVGIAMGTGTDVAMNSGQITLV
                     KGDLRGIAAARDISIDTVRNMRQNLLFAFVYNGIGVPIAAGVLYPITGWLLSPLIAAL
                     AMSLSSASVIFNALRLRRARG"
     gene            complement(8456..8959)
                     /locus_tag="PK518_07250"
     CDS             complement(8456..8959)
                     /locus_tag="PK518_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRM48749.1"
                     /translation="MTLKPLILGAVGLLALLLVAGVGYFSLRASNRPVTPPPHSMRPD
                     DPQVLRVGARIYAQQCAACHGAKGEGQPNWRDRGSDDLLPAPPHDPSGHTWHHPDEQL
                     FAITKQGLAQLINQPDYRTAMPIYGGVLSDDEIVAVLSWIKAQWPPEIRRRHDEINVQ
                     YRQSLSR"
     gene            complement(9015..9662)
                     /locus_tag="PK518_07255"
     CDS             complement(9015..9662)
                     /locus_tag="PK518_07255"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRM48750.1"
                     /translation="MEHTGSSYGLWTLVVLNVAIFVMFAFSFFKPASARDWRSFGAFT
                     AFIVALFVEMYGFPLTIYFLSGWLGQKLPGVDLLNHNAGHLLELLFGWGGDPHLGPFH
                     ILSYLFIGGGFWLLAAAWPVLYEAQRQGRLARTGVYARVRHPQYIAFVLIMFGFLLQW
                     PTLLTLLMFPVLVVMYARLAHNEEQESGRRFGQAWQEYAQHVPRFIPRWGAWVQP"
     gene            complement(9667..9945)
                     /locus_tag="PK518_07260"
     CDS             complement(9667..9945)
                     /locus_tag="PK518_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HRM48751.1"
                     /translation="MTHDHASHPQSVQTGGFWRSRYAIGLLVFGAVAGYFLWTEHRAH
                     LAQWWPYAFLLACPLMHVFMHKGHGGHGGHGGGAGATGQGTGQNSDRG"
     gene            complement(9956..10426)
                     /locus_tag="PK518_07265"
     CDS             complement(9956..10426)
                     /locus_tag="PK518_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48752.1"
                     /translation="MVHIRHTLIGLCVAAASVGVLAQGADGHKDHHPAETTAPAAAAP
                     APAAAPPSQAMSPDMMAAMEQRMRAMHEKMAAAKTPQERQALMAEHMKTMQDGMRMMK
                     SMHGQMASGNPPAGMAGMQHDPQMMEKRMAMMESMMQMMMDRMSMPQTSTPGTK"
     gene            complement(10501..10842)
                     /locus_tag="PK518_07270"
     CDS             complement(10501..10842)
                     /locus_tag="PK518_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HRM48753.1"
                     /translation="MNTIQRLLTLSALAMGLALPMAGHAQVSPSPGQVAAPVSDSMTD
                     GEVKKVDPDNGKITLKHGDIKNLDMPGMTMVFTVRDKGQLTKLKPGDKVQFMVVQEGG
                     KMVVTDIQPAH"
     gene            complement(10922..11419)
                     /locus_tag="PK518_07275"
     CDS             complement(10922..11419)
                     /locus_tag="PK518_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HRM48754.1"
                     /translation="MKFTRSTISQLFAMLALAASGAAFASGNHAGGHGHDEGGETAIG
                     KPGVAAKASRTITIEMSDKMRFTPSDLTVKKGETVRLIVKNTGQLKHELSLGTQEELM
                     EHLEQMKKFPGMEHDEPGKITLEPGKQGEIVWQFTKAGVVDFACLLPGHYEAGMKGAI
                     KVGRK"
     gene            complement(11453..12865)
                     /locus_tag="PK518_07280"
     CDS             complement(11453..12865)
                     /locus_tag="PK518_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HRM48755.1"
                     /translation="MTSRRDFFKVAGIAGGAVAAGAVSRVAMAALPEPVIQTSPNTMA
                     PLIPDSGRPYNPVVTLNGWTLPWRMNQGVKEFHLVAEPVVREMAPGMKAHLWGYNGQS
                     PGPTIEVVEGDRVRIFVTNRLPEHTAVHWHGQRLPNGMDGVTGLTQPAIPSGKTFVYE
                     FVARRPGTFMYHPHADEMVQMAMGMMGFWVTHPKAKHSLIDEVQRDFCFLLSAYDIEP
                     GAATPKVAEMTDFNLWTWNSRVFPGIDSLNVRLNDKVRIRIGNLTMTNHPMHVHGHEF
                     TVTGTDGGPVPKSARWPEVTTDVAVGQMRQIEFVADEEGDWAFHCHKSHHTMNAMGHN
                     VPTLIGVDHSGLSKKINQLIPDYMVMGERGMADMTEMEMPIPDNTIPMMTGEGPFGSV
                     EMGGMFSVLKVRRDQKPGDYTNPGWFKHPAGTVAHEVTGPMAEPARFSAEGGQSMPRQ
                     RKPATPTEVKVRKPASHGEH"
     gene            complement(12882..14312)
                     /locus_tag="PK518_07285"
     CDS             complement(12882..14312)
                     /locus_tag="PK518_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRM48756.1"
                     /translation="MMRLRASHARLALSALGLAVLTGCASVSLEQNIGRVNDEAGGFT
                     EGKLALARTQEERDRRAQATEALLAQPLGQKEAVQLALSNSPSLQALLAQGWAEAADA
                     AQAGRIANPIFDFERMTAGPELGLSRALSFGLLDLLTLPTRYGIATRRIEQSQLRLTG
                     NVVDQVTGVRQAWVRAVAAQQALQYAKQVYDSAEAGAELARRMQGVGNFNRITRAREQ
                     AFYADAATRLSETQHQVTARREELVRLLGLNESQAQALKLPERLPDLPKQALDPQAVG
                     ALATRARLDLRLAQTALDAAAKAQGLNMVTSFTDIELTGRRNTTFDNAAGTRGTARGW
                     EIAVRLPVFDWGGMQRDAMNARTLAAANQLEAAARAAGSSLRESYSAYRTAYDVARHY
                     RDEVVPVRKVIAEENQLRYNGMLISVFELLADARDQVGAVTAALDAQRQFWLADAALQ
                     ASVIGRPTNTTLSVTTSAPSDAAAGH"
     gene            complement(14309..14644)
                     /locus_tag="PK518_07290"
     CDS             complement(14309..14644)
                     /locus_tag="PK518_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48757.1"
                     /translation="MATSNHLPPFRALLPWTLALFAGAAFAQAPADTEAPQPSLPTKL
                     QYSSAIGAYQTYADQQVQSWREANDRVGQIGGWRAYAKEIKTGKSASVKDATPSSDPH
                     VGHHGGGTP"
     gene            14801..15484
                     /locus_tag="PK518_07295"
     CDS             14801..15484
                     /locus_tag="PK518_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HRM48758.1"
                     /translation="MKILIVEDEPKTGEYLRQGLSEAGYVADLVPHGTDGLHMALHGA
                     YDLVILDVMLPGLNGWQVLQSLRERGLQMPVLFLTARDQVEDRVKGLELGADDYLVKP
                     FSFAELLARVRIILRRGPAGNEGTMLRVADLELDLLRRRVSRNGKRVDLTAKEFGLLE
                     LLMRRHGEVLPRSLIASQVWDMNFDSDTNVIEVAMRRLRMKIDEGHPVKLIQTVRGMG
                     YVLDVPQEE"
     gene            15485..16894
                     /locus_tag="PK518_07300"
     CDS             15485..16894
                     /locus_tag="PK518_07300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HRM48759.1"
                     /translation="MLLRVRWGRLPLTQRLTLLFTAVAASVVLGLGALFLVETERHFI
                     ELDRMALQDKQHLIEEILRNANSADDARRRLGEALSYHHDLYAQVQDGQGAVVFQSRG
                     FIPTMRGDMPLRAGENKVFGVLRHDGARFHTLVFKAAPAYSSTPLMVWIAADTDHHTQ
                     FLNGLRRSLALYVLAAIAICGLLSWFAARQGLAPLRDMKSRAAKVTGQKLGERMPVQA
                     VPVEMADLAQELNRMLDRLQEDFQRLTDFASDLAHELRTPISNLLTQTQVALATKRDA
                     ATYRDILASNAEEFQRLARMVSDMLFLAKTERGVDLPHKERFSVRQDAQALLDFYEAV
                     AEEKRIRLRLEGDGEVEGDRLMFRRAVSNLLSNALRHTPEAGDITIRIATTVQATTVA
                     VENTGADIDAKILPRLFDRFYRADASRAHPDSDGSGLGLAITRAIAQAHGGQVTATSG
                     QGRTCFALVFPHRVAKPRS"
     gene            17020..>17116
                     /locus_tag="PK518_07305"
     CDS             17020..>17116
                     /locus_tag="PK518_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HRM48760.1"
                     /translation="MTQQRLSLSSMIAAAAAVAALGLPGMASAAYE"
BASE COUNT         2860 a         5643 c         5513 g         3100 t
ORIGIN      
        1 gccaccgtgc ccagcgtgat ctgttgcccc atgggggtga agatgggcag ttgcttcagc
       61 ctttccggtg aatccctcca ttcacgcgcg tatcgcagat tgatcgggaa gcgcgccagc
      121 ccctccacgg tctccgacac gttttcgcca ccgacggcac cggcgacgac cgcctggaca
      181 tcggccacgt tcagcccata gcgccccgcc gccacgcggt cgatctgcac gtccacatac
      241 cggccgccgg tcagccgctc cgccaacgat gacgacacgc ctggaatgcc cttcgcgacc
      301 tgctccacct ctgccgcgat gcgatcgatc acatgcaggt cgttgcccgt caccttgacg
      361 ccgatcggac tcttgatgcc ggtagccaac atgtcgatgc ggttgcggat cggcggaatc
      421 cagatgttgg acaggccggg gattttcacc gcctgatcca gctcctcgat gagcttctcg
      481 ggcgtcatgc cggcgcgcca ttgctctcgg ggcttgagct tcaccgaggt ctcgaacatc
      541 tccagcggcg cggggtcggt cgcggtttcc gcacgacccg ccttgccgaa cacggtttcg
      601 acctcgggca ccgtcttgat catgcggttg ctgagctgca gcagctccgt ggcgcgttgc
      661 gccgacagcc ccggcaaggc cgagggcata tagagcagat ccccctcatc cagccggggc
      721 aggaattcgc caccaagccg ggccagcggc caggccgtgg tcgccagcgc cagcaccgcg
      781 atcagcaagg ttgccttggg ccagcgcagc acccattcca ggctgggccg gtacaccgca
      841 atcagcatgc gggtgatggg attcttctgc tcgtcgggaa tgcgtccgcg aatccagtag
      901 cccatgagca ccgggatcag cgtcacggac aggcccgccg ccgccgccat ggcgtaggtc
      961 ttggtgaacg ccagcggccc gaacaggcgt ccctcctggg cttcgagggt aaagacgggg
     1021 atgaaggaca aggtaatgat gagcagcgag aagaacagcg ccggaccgac ttcctccgcg
     1081 gcccgcgtga tcacctccca gcgttccttg ccttgcagcc gctgatccgg atgcgcgtgt
     1141 tgccaggctt ccagcttctt gtgcgcgttc tcgatcatga ccacggccgc atccaccatc
     1201 gcgcctatgg cgatggcgat gccgcccagc gacatgatgt tggcattgat cccctggtag
     1261 cgcatgatga ggaaggccat catcaccccc agcggcaacg aaatgatggc caccagcgcc
     1321 gagcgcaggt gccacaggaa cagcacgcag accagcgcca ccaccagaaa ctcctcccct
     1381 agcttgatgg tgaggttgcg gatggcgcgc tcgatgaggg cactgcggtc gtaggtggtg
     1441 acgatctcca ccccctgcgg caggctggcc tgcagctcgc ccagcttggc cttgacggcg
     1501 gcaatcgtct cctgggcatt cttgcccgaa cgcaggatga ccacgccgcc ggcgacctcg
     1561 ccctcgccat cgagttcggc gatgccgcgc cgcatctccg gcccgatctg tagcgtggcc
     1621 acgtcgccca ggcgcaccgg cacaccgccg cgcgccacca gcggtactgc acggaaatca
     1681 tccagggttt tcagatagcc gctggcgcgc accatgtatt cggcccccgc cagctccagc
     1741 accgagccgc cagtttcctg attggcggcc accagcgccg cgcgcacctg ctcctggctc
     1801 agtccgtagg aggcgagctt gaccgggtcg agcacaacct ggtactgctt gaccatgccg
     1861 cccaccgagg ctacctctgc cacattgggc aggctcttga gctcgaactt cagaaaccag
     1921 tcctgcagcg cgcgcaactg cgccagatcg ttcttgcccg tgcggtccac cagtgcgtac
     1981 tggaagatcc agcccacccc cgtggcatcc ggccccaggg ccggcttggc tgtggccggc
     2041 aggcgtccct gaacctggtt caggtactcc agcacccgcg agcgcgccca gtacaggtca
     2101 gtgccatctt cgaacaggac atagacgaac gagtccccga aaaacgaaaa gccgcgcacc
     2161 gtcttggccc ccggcaccga cagcatggtc gtggccaggg gataggtgac ctggttttcc
     2221 acgatctgcg gcgcctggcc ggggtagctg gtacggatga tgacctgcac gtcggacagg
     2281 tccggcaagg catccacggg cgtgctgcgc acgccccaca ccccccaggc cgtaagcatg
     2341 gccgtggcca gcagcaccag aaagcggttc gccaccgacc agcggatcaa tttggcaatc
     2401 atggcttgat cccttcgatg gcggtgatca cgtagtcctc tccctgcttg gagaaggtga
     2461 agcgcacctg ttgcttgggt gccaggcctg cggcgaggcg ggggtcggcc agcttgaagc
     2521 ccatggtcat ggcgggccac ttgagggcgg ggatggcgtc atgcgccagc gtcagctcgg
     2581 cagtggagac ttcttccacc acaccgcgca ccgtgtaggc cggggccgcg ccatgcgccc
     2641 catgctccgg cgtcgccccc ggcgctgcgg gcaacaggcc gcgcaggctg gcctcggagt
     2701 cgatcaggaa ctgtgccgag gccaccacct gctgccccgc ctccagtcca ccttgcacca
     2761 cgagttggtc gccgatctcc gcccccagct gcacctccac ggggtggaat ttgccagggc
     2821 catcgaccac ataggccagg gcgcgcttgc cggtgcggat gacggcttcg ctgggaacca
     2881 gcagtgcggg ctgctcgcgg cccttgagcg tgatctggcc ggacatgccg gccttcaggc
     2941 gctggccggg gttggccagt tccagccgca cgcgcaccgt gcgcgtgtcg ctgttggcct
     3001 gcggcaggat gctcacgatg cgcgcccgca gcacctcgcc gggaaacgcc gccaggcgca
     3061 cctgggcttc ttgccccagt tgcagcgggc cgctttgcgc ctcgggtact gcggcctcga
     3121 tccagacggt gcccaggcca ttcacgcgcg ccaggcttgc gcccgcagag atagtcatgc
     3181 cctggcgcac catgagctcg gccaccagcc cgccctgggg cgtggtcacg gtgtagacgc
     3241 ccctgggctc gcccgtgcgc tccacctggg ccaccagggc ctgcggcatg cccagcagca
     3301 gcagcctttg ccgggcggcc gccgtcagcg actcatcctt gagcgcacgc accgccagaa
     3361 attcgcgctg cgccgccacc cattccggca gcagcagatc cgccagcggc gcgccggccg
     3421 cgatcacatc gcccggcgcg cgcgcataga cccgctccac aaagccggcg gagcgcgcct
     3481 gcacgatgct gacatcgcgg tcgttgagca gcaccgttcc caccgcgtcc acatcggagc
     3541 ctatggcgcg ctgcaccacc tcggccgtgc gcagacccag cgcctgcacg gtctgggccg
     3601 atacgctcag gcccacgctg tcctgggcat cctcgtcggc atacttgggt accagatcca
     3661 tgtccatgaa aggagacttg ccgggcttgt cgaacttctg cgtcggcgac atggggtcgt
     3721 accagtacag caccttgcgc tcggccgccg cgccggcttg gggcgctgct ccgtccacag
     3781 catgtgtgct gctgcgccat tgggacaggc cccaacccgc ggccacgccg atggcgatca
     3841 gcgtcaggcc cgcgagcgat tttttcagag agttgttcat ggttgtgaga tccgttaatc
     3901 agcgatcagg ctgttcaggc gcgtcgtcac gccgtcgcgc tgggcttgca ggtcgatcag
     3961 gcgcaggcgc gtctccagca cctgagcgcg ggcgccgagc acggcgccca ggtcgccctt
     4021 ggcggcccgg tagttgccca gcgcgagctc cgctcgcccc tgcgccaact gcagcccggt
     4081 ggacttgagg cgctcgatct ggctgtccag ggcctgcaat tcggccgcgc tgtcgtcaag
     4141 ctcctgcaaa tgccgccgcg ccacgtcttc ctgctcggct tccaggcgct ccagctcgcg
     4201 ctgcttggcc tggatcatcg gggtctggcg tcgatccttt tgccatggca gatcgaacgt
     4261 cacctggaac gacaccatgt cgccccatcg acgatcgcgt cggctatagg caatttccca
     4321 ggaccagtcg ccacgcgatt ccgactgcgc ctcgtgggat tccgcccgtg ccatgctttg
     4381 catggtcggg tacagcgcca gctcggcgtg gctggatagc tcggcgcgca actgcgcaac
     4441 gggatggatg agcggtccgg tgccgccctg tagcgattcg tcggcgcgcg gcccgaccca
     4501 gcgccgcagc atggctcgcg ccttggaccg gtcgcgttgc aggtcgtcgc gccggtccga
     4561 cagcgccagc gcctcctgct gcgcggccag caggtcgcct gcctgggccg atccgcccgc
     4621 cacccgcgcg ggcaggctgt cttgcaggcg ctggttctcc gccagcagct ccgtcagcaa
     4681 ctgttcccgc tgctcgactg cctgcgcggc gatccaggcc agaccgagtt cctgccgtat
     4741 ctgcaggcgc tgcagcacca gcgccgcgcg ttcgcgttcc acgcgcgcct gggcgctggc
     4801 caccttggcc gcgcgcttgg cctgattggg aacctcctgc atcagggcca ggcgctgccc
     4861 ggtcatggac tcgcgcgtca ggctccagcg gtccatgccg ctgatgggga ggttttcaag
     4921 accgacggag agcttgggat cgggtagcgt gccggcggcc ttctgcgcgg actcgaccgc
     4981 gtcgatctgc aatcgctgcg cggaaacccg cgggctttgc tgttcagcga tttctcgtgc
     5041 ttcggcaaag ctgagcgcgt aggtccggct ggcggcgaac atgctgccag ccagggcaat
     5101 gctcaagatg ctcagtcgat gtatccatgt gcggcgcggg atcgcacgca atacgccatg
     5161 tccaggcgcg gggcgacgct cgccgggagc gccggaggaa gaaataagca tgatggtcct
     5221 cgaaattgat agccacgaag tcacgtgttc tgccgtgcca atgcacgacg ggcacgacct
     5281 ggtgtgtcaa ttgcgcagga cttggaaagt gagatagagg gggagagagc ccgttcggtg
     5341 gtcgagccac gctgggtcaa acctggcgcc ggtcgatgat tgcgggtcgg gcaccacggg
     5401 aagcaggtag gcaatccaga ccgcatgggc cgccgcaata gatggaacgt ccagcccttg
     5461 cggagacggc gcgggcttgc cgtcaccgct gcaatgggca cggcataact gcggagactc
     5521 gtcgtccatc gtccctggca tggcatggca gtccggcatg gcggacatgc cggcggcttc
     5581 gtccatggcc atgcccgcca tctgcatcga tgccgcgtgc gtatccgcat ccagcttggg
     5641 gcaggcatac atcgccagcg ccatctgcga gaacaacaag gtcacgatga ggccgccgac
     5701 gattcggcgg ccccattgtg tgacgatgcg tcggatggaa accatggtgg gatgttcgct
     5761 gtgctggatg tgtgacaact tgccgcggat cagagagccg tcccaggaaa gaagtgccac
     5821 catggctcat ctacccgcgt gacagcccag tcaccgcgca tgcgaggtca ccgagaccct
     5881 ggattcattt caccggaatc caacttgtcg gcgcgtttcg atctcggcca ctccgagaac
     5941 gcaagcacgt acagtgcgat caggttgacc atggggacga tcatcagcag gccgagccag
     6001 ccggaaaagc ccgccttggc aaagatcttc caaaacggca gcacgatcac caccgcaccc
     6061 agcagcatca tcagccagtg ggcgccaaac cgccacgcca tgtcgttcat atacattccg
     6121 ttcatcatga tcgatctcct ttctttgatt gtctcgatgc gggttagcct cgtgcgcgcc
     6181 gcagccgcag cgcgttgaag atgacggagg ccgagctcag gctcatggcc agtgctgcaa
     6241 tcaatggaga cagcaaccag cccgtgatgg gataaagcac gccggcggcg atgggcacgc
     6301 cgataccgtt gtaaacgaag gcgaacaaca ggttctggcg catgttgcgc acggtgtcga
     6361 tggagatgtc gcgcgcggct gcgatgccgc gcagatcgcc cttgaccagg gtgatctggc
     6421 cgctgttcat ggccacgtcg gtgccggtgc ccatagcgat gcccacgtcg gccttggcca
     6481 gggcaggtgc gtcgttgatg ccgtcgccgg ccatggcgac gacgtgcccc tccttctgca
     6541 gccgctccac caaggccagt ttgtcggccg gcttgacttc gccatgcact tcgtcgatgc
     6601 ggagcttagc gcccaccgcc cgggccgtgg tcaggccgtc gcccgtcgcc atgacgatgc
     6661 gcaggccact gtcgtgcagc gtcttgatgg cgtcggccgt ggtggccttg atcgggtcgg
     6721 tgacggccag gatgcctgcc agccgcccgt ccacggccag gtgcatcacg ctgccaccct
     6781 cgctgcgcag gcgctctgcg tccgccttga gcgcatcggt ggccacgttc tcctgctgca
     6841 tcaaggcggt gttgcccagc accagatgct tgccttcgac cgtgccgcgc acgccgatgc
     6901 cgctgcccga ttcgaagtcg tcggccttgg tcaggctcag ccctttgtcc cgcgcggcgc
     6961 ggacgatggc atccgccagc gggtgctcgc tgccctgatc cagactggcc gccaggcgca
     7021 gcacctcatc cggcgtgtag cccgggtcgg cgacggcggt gtcgaaggcc ggcttgcctt
     7081 cggtcagggt gccggtcttg tccacgatca gcgtatccac ttcgcgcatt ttttcgatgg
     7141 cgccggcatc gcggaataga atcccctgcg ttgccgcgcg gccggtggcg accatcacgg
     7201 acatcggggt cgccaggccc agcgcgcaag ggcaggcaat gatcagcacc gccacggcgt
     7261 tgatcaggcc gaacacccag ctcggctcgg ggccgaagaa tccccagacg aagaaggtcg
     7321 cgattgcgat cagcaccacc acgaccacga acttgccggc caccacgtcg gccatgcgct
     7381 gcatcggcgc cttggagcgc tgggcattgg ccaccatctg cacgatctgc gacagcatgg
     7441 tggcggcgcc gaccttctcc gagcgcatca ccagtgcgcc gctggtgttc atcgtggcgc
     7501 caatcaggcc gtcgccggcg cgcttggtca ccggcacggg ctcgccggtg agcatggatt
     7561 catcgacggc actgctgccc tcggtcacca cgccatcgac gggcactttc tcgccgggcc
     7621 gcacgcgcag caggtcgccg acgtgaacat ggttcaaagg cacgtcctcc tcgctgccgt
     7681 cggcattgat gcggcgcgcc gtcttggggg ccaggcccag cagagccttg atggccgccg
     7741 aagtctgcga gcgcgccttc aattcgatga tctgacccag catggtcagc gagatgatga
     7801 cgaccgccgc ctcgaaatag acggcgacgc ggccgtgggc gatgaacgag tctggaaaca
     7861 cgccgggcgc gagggtggcg accacgctgt agacaaaggc ggcgccggtg cccaggccga
     7921 tcagcgtcca catattgggg ctgcggttga cgatggactg ccagcagcgg acaaagaatg
     7981 gccatcccgt ccacaacacc accggcagcg acagcaccag ctcgacccag gtctgggtct
     8041 tcatgtcgaa ccagccgagg cggtggccga acatcgccag gatggtgacc accacggtca
     8101 gcggcagggt ccaccagaag cggcgctgaa aatcgatcag ttcgctgttg tcctcctcat
     8161 ccagcggaat caccggttcg agcgacatgc cgcatttcgg gcaattgccg ggatgatcct
     8221 gctgcacttc cgggtgcatg gggcaggtgt agatggtgcc cgccggggcc tccatgggca
     8281 ccgcggcaga agttgccggg gtctgttcgc ctgcggtatg gccgtggtga tgcaggtgtt
     8341 ggttcatgcc catgcctttg ttgagttatc tcgattcttg accttgccat cgcggcaagg
     8401 tcaagtccct ttccttgtgg acgcattcgg tccgcattgg cccatgcccc gatggctatc
     8461 gggacaaaga ctggcggtac tggacattga tttcgtcgtg gcggcgccgt atctctggcg
     8521 gccattgcgc cttgatccat gacagcacgg ccacgatctc gtcgtcgctc aatactccac
     8581 catagatggg catcgccgta cggtagtcgg gctgattgat caattgggcc agtccctgct
     8641 tagtgatggc gaacagttgc tcgtcgggat ggtgccaagt gtggccgctg ggatcgtgcg
     8701 gcggcgcagg aagcaagtca tccgatccac gatcacgcca gttgggctgg ccctcgccct
     8761 tggccccatg gcaagccgca cactgctgcg cgtagatgcg ggcgccgacg cgcagcacct
     8821 ggggatcatc gggacgcatg ctgtgcggcg gcggcgtcac tggccggttg ctggcgcgca
     8881 gggaaaagta acccacccct gcgaccagca gcaaggccag caatcctacg gcccccagga
     8941 tcagcggttt caacgtcatc gcaacttcct tgatttcatg ctgatggtca cattctggtc
     9001 atcggatgcg tcgctcaagg ctgaacccat gcgccccagc gcggaatgaa acgcgggaca
     9061 tgctgcgcat actcctgcca agcctgtcca aaacggcgtc cgctttcctg ctcctcgttg
     9121 tgcgccaggc gggcatacat caccacaagc accgggaaca tcaacagcgt cagcaaggtc
     9181 ggccattgca ggagaaagcc gaacatgatc agcacgaagg cgatgtactg cggatgccgc
     9241 acccgagcgt aaacaccggt gcgcgccagc cgcccctgcc gctgggcctc gtagagcacg
     9301 ggccaagccg cggccagcag ccagaagcca ccgccaatga acaggtagct gaggatgtgg
     9361 aatggcccaa ggtgcggatc accgccccag ccgaacagca gctccagcag gtgaccggcg
     9421 ttgtggttca gcaggtccac gcccgggagc ttctggccca gccagcccga caggaagtag
     9481 atggtcagcg ggaagccata catctccacg aacagcgcca cgatgaacgc tgtgaacgcc
     9541 ccgaaactgc gccagtcgcg cgcgctggcc ggcttgaaga agctgaaggc gaacatgacg
     9601 aagatcgcca cgttgagcac caccagagtc cacaggccat acgaagatcc cgtgtgctcc
     9661 atggcctcag cccctgtcgg agttttgtcc agtgccctgg ccagtggctc cggcgccacc
     9721 accgtggccg ccatgaccac catggccttt gtgcatgaag acatgcatca gcgggcaggc
     9781 cagcaggaag gcatagggcc accattgcgc caggtgggca cggtgttcgg tccataggaa
     9841 gtacccggcg acggcgccga acacgagcag gccgatggca tagcgcgagc gccagaaccc
     9901 gccggtctgc acactctgcg gatgggatgc atggtcatgg gtcatgacgg cctcctcact
     9961 ttgtgccggg agtggaggtt tgcggcatgg acattcgatc catcatcatc tgcatcatcg
    10021 actccatcat ggccatgcgc ttttccatca tctgcgggtc gtgctgcatg ccagccatac
    10081 cggcgggtgg gttgcccgat gccatctggc cgtgcatcga cttcatcatg cgcatgccgt
    10141 cctgcatggt cttcatgtgt tcggccatca acgcctggcg ctcctggggc gtcttggcgg
    10201 cggccatctt ctcgtgcatg gcccgcatgc gttgttccat cgcggccatc atgtccgggc
    10261 tcatggcctg cgatggcggt gctgcagccg gcgccggagc tgccgccgca ggggcggtcg
    10321 tttccgccgg gtggtggtcc ttgtgcccgt cggcgccttg cgccagcacg cccacggagg
    10381 cggcggcgac acagagaccg atcagggtat ggcgaatatg aaccatgatg cttccttctt
    10441 gcgttgcagg ggttggctag ggcagccgcg tccggcggca tgatgcggtg gtgactctgc
    10501 tcagtgcgcg ggttggatgt cggtgacgac catcttcccg ccttcctgga cgaccatgaa
    10561 ttgcaccttg tcgccgggct tgagcttggt gagttggccc ttgtcgcgaa cggtgaagac
    10621 catggtcatt ccgggcatgt ccaggttctt gatgtctcca tgcttgaggg tgatcttgcc
    10681 gttgtccgga tcgaccttct tgacctcgcc atcggtcatg gagtccgaca caggcgccgc
    10741 cacctggcct ggactggggc tgacctgggc atggcccgcc atcggcaggg caaggcccat
    10801 ggccagggcg gagagggtga gcagacgttg gatggtgttc atggtgacct ctgggtgaat
    10861 ggatggatct ataggctggc tcggccatgg ctgggccggc tgtggtgggc gtttcaggcc
    10921 atcacttgcg gcccaccttg atggcgccct tcatgccggc ttcatagtgg cccggtagca
    10981 ggcaggcgaa atcgacgaca cccgccttgg tgaactgcca gacgatctcg ccctgcttgc
    11041 ccggctccag ggtgatcttg ccgggctcgt cgtgctccat cccggggaat ttcttcatct
    11101 gctccaggtg ttccatcagt tcctcctgag tgccgaggct gagttcgtgc ttgagctgtc
    11161 cggtgttctt gacgatgaga cgcacggtct cgcccttttt gaccgtcagg tcggaaggcg
    11221 tgaagcgcat cttgtcgctc atctcgatgg tgatggtgcg gctggctttg gcggcgacac
    11281 cgggcttgcc aatggccgtc tcgcccccct cgtcatggcc gtggccgccc gcatggttgc
    11341 cgctggcaaa ggctgccccc gatgcggcca gcgcaagcat ggcgaagagt tgagaaatag
    11401 tggatcgcgt gaatttcatg ggtgttctcg ggttggaaat cgaagggaaa attcaatgct
    11461 cgccatgcga agcgggcttg cgcaccttga cttcggtcgg cgttgccggc ttgcgctggc
    11521 gcggcatgga ctggccgccc tcggcactga aacgcgccgg ctcggccatg gggccggtga
    11581 cctcatgcgc caccgtgccg gcgggatgct tgaaccagcc cgggttcgtg tagtcgcccg
    11641 gcttctggtc acggcgcacc ttgagcacgc tgaacatgcc gcccatctcc accgacccga
    11701 acggtccttc accggtcatc atcgggatgg tgttgtcggg aatcggcatt tccatctcgg
    11761 tcatgtcggc catgccgcgt tcgcccatga ccatgtagtc cgggatcagc tggttgatct
    11821 ttttggacag gccgctgtgg tccacgccaa tgagcgtggg cacgttgtgc cccatggcgt
    11881 tcatagtgtg atggctcttg tggcagtgaa aggcccagtc gccttcctcg tcggcaacga
    11941 attcgatctg gcgcatctgg cccacggcca cgtcggtggt cacctcgggc cagcgcgcgc
    12001 ttttgggcac ggggccgcca tcggtgccgg tgaccgtgaa ttcatgacca tgcacgtgca
    12061 tcgggtggtt ggtcatggtg aggttaccga tgcgaatgcg aaccttgtca ttgagacgga
    12121 cattcaagga gtcgatgccc ggaaagacac ggctgttcca tgtccacagg ttgaagtccg
    12181 tcatttccgc gaccttgggc gttgcagcgc ctggctcaat gtcatacgca ctgagcagga
    12241 aacagaaatc ccgctgcact tcgtcgatca aggagtgttt ggctttcggg tgcgtgaccc
    12301 agaagcccat catgcccatg gccatttgca ccatctcgtc ggcgtgcggg tggtacatga
    12361 aggtgcccgg gcggcgcgca acgaactcgt agacgaaggt cttgccggac ggaatggctg
    12421 gctgcgtcag accggtcacg ccatccatgc cgttgggcag gcgctggcca tgccagtgca
    12481 cggctgtgtg ctcaggcagc ctgttggtga cgaagatgcg cacgcggtcg ccctccacca
    12541 cttcgatggt cgggccgggg ctctgaccgt tgtagcccca caggtgggcc ttcatgcccg
    12601 gagccatctc gcgcaccacg ggttcggcca ccaaatgaaa ctccttgacg ccctggttca
    12661 tccgccaagg cagcgtccag ccgttgagcg tgaccacggg gttgtacggc cggcccgaat
    12721 cggggatcag tggcgccatg gtgtttgggc tggtctgaat gacgggctcg ggcaaggccg
    12781 ccatggcgac acggctgacc gcgccggccg cgacggcgcc gcccgcaatc ccggcgacct
    12841 tgaaaaaatc tcttctggat gtcatggcaa tcgtctgtgc atcagtgacc ggcagccgcg
    12901 tcgctgggcg cgctggtggt gactgacagg gtggtgttgg tggggcgccc gatcacggag
    12961 gcctgcaagg cggcatcggc cagccagaat tgcctctgcg cgtccagggc cgccgtgacg
    13021 gcaccgacct ggtcgcgcgc atcggccaac agttcgaaca cgctgatcaa catgccgttg
    13081 tagcgaagct ggttctcctc cgcgatcacc ttgcgcaccg gcaccacttc gtcgcggtaa
    13141 tgccgcgcca cgtcgtaggc cgtccgatag gccgagtagc tttcacgcag gctggagcct
    13201 gcggcacgcg ccgccgcttc gagctggttg gcggccgcca gggttcgggc gttcatcgcg
    13261 tcacgctgca tgccgcccca gtcaaagacg ggcaggcgca ccgcgatctc ccagccgcgc
    13321 gcggtgccgc gggttcccgc agcgttgtcg aaggtggtat tgcggcgccc ggtgagttcg
    13381 atatcggtga agctggtcac catgttcaag ccttgggcct tggccgcggc atccagcgcg
    13441 gtctgcgcca ggcgaaggtc caggcgcgcc cgggtggcca gcgcgccgac cgcctgcggg
    13501 tccagcgctt gcttgggcaa gtcgggcagg cgctcgggca gcttcagcgc ctgcgcctgg
    13561 ctctcattca gccccagcag gcgcaccagc tcttcccggc gggccgtgac ctggtgctgg
    13621 gtctcactca gccgggtggc cgcatcggca tagaaagcct gctcgcgcgc acgggtgatg
    13681 cggttgaagt tgcccacgcc ttgcatgcgc cgggccagct cggcgcccgc ttcggcgctg
    13741 tcatagacct gcttggcata ttggagcgcc tgctgtgcgg ccacggcccg cacccaggcc
    13801 tggcgcaccc cggtgacctg gtccaccacg ttgccggtca ggcgcaattg agattgctcg
    13861 atgcgccggg tggcgatgcc gtaccgggtc ggcaaggtca acagatccaa caggccaaag
    13921 gacagcgctc ggctcaagcc cagttccggg ccggccgtca tgcgctcgaa atcgaaaatg
    13981 ggattggcaa tccgccccgc ctgggcggcg tcggccgcct cggcccagcc ttgcgccagc
    14041 agggcctgca gggatgggct gttgctcaag gccagctgga cggcctcttt ctgccccaaa
    14101 ggctgcgcca gcagggcttc ggtggcttgc gcacgccggt cgcgctcctc ctgggtgcgt
    14161 gccagtgcca gtttgccttc ggtgaagccg cccgcttcgt cgttgacgcg gccgatgttc
    14221 tgctccaggc tgacgctggc acatccggtc agtaccgcca gcccgagtgc agacagggcg
    14281 agtctggcgt gcgaggcacg caaacgcatc atggcgtgcc tcccccgtga tgacctacat
    14341 gcgggtcgct agagggggtg gcatccttga cggacgcgga cttgcccgtc ttgatttcct
    14401 tggcataggc gcgccaaccg ccgatttggc ccacgcgatc gttggcttca cgccaggatt
    14461 gcacctgttg atccgcatag gtctggtacg caccgatagc ggacgaatac tgcagcttcg
    14521 tcggcagtga aggctgggga gcttcggtat ctgccggggc ttgtgcaaac gccgcccctg
    14581 cgaacaacgc cagtgtccaa ggcaacaggg ccctgaaagg cggcaggtga ttggaagtgg
    14641 ccatggtctc ctctagaaga atctttgatc tcacgatagg gagattctgg agaggccacc
    14701 ctttcagcaa gatgtttgca acattacatt cctgtcagct tcatgccggc aggccttaca
    14761 cggggcacac ttgcgccaga accgcaaagg agccgaaccg gtgaagatat tgatcgtcga
    14821 agacgagccc aagactggtg aatatttgcg ccagggcttg agcgaggccg ggtatgtcgc
    14881 ggacctggtg ccccatggca ccgatgggct gcacatggcc ttgcacggtg cctacgacct
    14941 cgtgatcctg gatgtcatgc tgcccgggct gaacggctgg caggtgctgc aatcgctgcg
    15001 cgagcgcggc ctgcaaatgc cagtgctgtt cctgaccgca cgcgaccagg tggaagaccg
    15061 cgtcaaggga ctggagcttg gcgccgacga ctatctggtc aagccgttct cgtttgcgga
    15121 actgctggcc cgcgtgcgca tcatcttgcg ccgcggcccg gcgggcaacg aaggcaccat
    15181 gttgcgtgtc gccgatttgg agctggatct gctgcgccgc cgggtctcgc gcaacggcaa
    15241 gcgcgtggac ctgacggcca aggaattcgg cctgctggaa ctgctgatgc gtcgccacgg
    15301 cgaggtgctg ccgcggtccc tgatcgcatc gcaggtgtgg gacatgaact tcgacagcga
    15361 caccaatgtc atagaggtgg cgatgcgacg actgcgcatg aagattgacg agggccaccc
    15421 ggtcaagctc atccagaccg tgcgcggcat gggttatgtg ctcgacgtac cgcaggagga
    15481 ataggtgttg cttcgggttc gttggggccg gttgccgctg acacagcgcc tgaccctgct
    15541 cttcactgcg gtggcggcct cggtggtgct ggggctgggc gccttgtttc tggtcgaaac
    15601 cgagcggcat ttcatcgagc tcgatcggat ggcgctgcaa gacaagcaac acctgatcga
    15661 ggaaattctg cgcaatgcca actcggcgga cgacgctcgc cggcgactgg gcgaggcgct
    15721 gagctatcac catgatctct acgcccaagt ccaggatgga caaggagcag tggtctttca
    15781 atcccgaggg ttcatcccca ccatgcgcgg cgatatgcct ctgcgcgccg gtgaaaacaa
    15841 ggtgtttggg gtgttgcggc acgacggtgc ccggttccat acactggtct tcaaggccgc
    15901 ccctgcctac tccagcaccc ccctgatggt ctggatcgcg gcggatacgg atcaccacac
    15961 gcagtttctc aacggcctgc ggcgcagctt ggccctgtat gtgctcgccg ccatcgccat
    16021 ctgcggcctg ctctcgtggt tcgccgcgcg ccaggggctg gcgcccttgc gcgacatgaa
    16081 gtcacgggcc gccaaggtga cgggccagaa gctcggtgag cgcatgcccg tgcaggccgt
    16141 gccggtggaa atggccgacc tggcgcaaga gctgaaccgt atgctggatc ggctgcagga
    16201 ggacttccag cgcctgaccg atttcgcgtc ggacctggca cacgagctgc gcacccccat
    16261 cagcaatctg ctgacccaga cccaggtggc gctggcgacc aagcgcgatg ccgcaaccta
    16321 ccgcgacatc ctggcctcga acgccgagga attccagcgg ctggcgcgca tggtgtccga
    16381 catgctgttc ctggccaaga ccgagcgcgg cgtggatctg ccgcacaagg agcggttttc
    16441 cgtccgccag gacgcgcagg cgctgctcga cttctacgag gccgtggccg aggaaaagcg
    16501 catccggctg cgcctggagg gcgacggcga ggtcgagggc gatcgcctga tgttccgccg
    16561 cgcggtgagc aacctgctgt ccaacgccct gcgccacacg cccgaggccg gcgacatcac
    16621 catccgcatc gccaccaccg tacaggccac gacggtggcc gtggagaaca ccggcgccga
    16681 catcgacgcc aagattctgc cccggctgtt cgaccgcttc taccgcgccg atgcgtccag
    16741 ggcccatccg gattccgacg gctccggact gggcctggcc atcacgcgcg ccatcgccca
    16801 ggcccacggc ggccaggtca cggcgacctc gggccagggg cgaacctgct tcgccctggt
    16861 gtttccgcat cgcgtggcca agccgcgaag ctgacaacaa cttcatcggg ttgtccagat
    16921 tctgttgggg tgcgctccct acgatgaacc catctcctca acgcccgtcc agggcatgag
    16981 agatgcaagt ttcatccacc tttgtcagga gcttcaacca tgacccaaca acgcctctcc
    17041 ctttcttcca tgatcgccgc cgcagccgca gtggctgccc tgggccttcc agggatggct
    17101 tcggcagcat acgagc
//
LOCUS       DAOMTQ010000055        17037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_484_length_17037_cov_26.904016, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000055 DAOMTQ010000000
VERSION     DAOMTQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 17037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17037
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_484_length_17037_cov_26.904016"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..485
                     /locus_tag="PK518_07310"
     CDS             15..485
                     /locus_tag="PK518_07310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRM48761.1"
                     /translation="MSINATLFVQAIVFLILVWFTMTFVWPPIAKALDERAQKIADGL
                     AAADKAKTELAAADQRVKKELAAASNETATRLADAERRAQAIIEEAKARATDEGNKIV
                     AAARAEAEQQAIAARETLREQVAALAVKGAEQILRKEVNAGVHADLLNRLKTEL"
     gene            496..1038
                     /locus_tag="PK518_07315"
     CDS             496..1038
                     /locus_tag="PK518_07315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRM48762.1"
                     /translation="MAELATIARPYAEALYKACTGKTGVDLGGASAWVDELAAIAANP
                     QLRQLLDNPKVSAEQLFGLITGVARTALPDMAHNFLRTLLDNGRVQALPEIAAQFRAL
                     VNQGNGSSDAVVYSAFPLDANALAELGATLQKRFGRQLNLSVQTDESLIGGVRVVVGD
                     EVLDTSVKARLEQMKAALIA"
     gene            1084..2643
                     /gene="atpA"
                     /locus_tag="PK518_07320"
     CDS             1084..2643
                     /gene="atpA"
                     /locus_tag="PK518_07320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRM48763.1"
                     /translation="MQLNPAEISELIKSRIEGLAASSDIRNQGTVVSVTDGIVRIHGL
                     SDVMQGEMLEFPADADGQPSYGLALNLERDSVGSVILGGYEHISEGDTVKCTGRILEV
                     PVGPELVGRVVNALGQPIDGKGPINAKMTDVIEKVAPGVIARQSVDQPLATGQKSIDS
                     MVPVGRGQRELIIGDRQTGKTAVAIDAIIAQKGQGVTCIYVAIGQKASSIKNVVRALE
                     QAGAMEYTIVVAASASESAAMQYVSAYSGCTMGEYFRDRGQDALIVYDDLSKQAVAYR
                     QVSLLLRRPPGREAFPGDVFYLHSRLLERAARVNADYVEAFTKGEVKGKTGSLTALPI
                     IETQAGDVSAFVPTNVISITDGQIFLETSLFNAGIRPAINAGISVSRVGGAAQTKLIK
                     GLSGGIRTDLAQYRELAAFAQFASDLDEATRKQLDRGARVTELLKQPQYSPLPISLMG
                     SSLFAVNKGFLDDVDVKKVLSFESGLHQFLKTSHAALLERLEANRAFDKEGKDEAELT
                     QAITAFKKTFA"
     gene            2663..3529
                     /gene="atpG"
                     /locus_tag="PK518_07325"
     CDS             2663..3529
                     /gene="atpG"
                     /locus_tag="PK518_07325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRM48764.1"
                     /translation="MAAGKEIRGKIKSVENTKKITKAMEMVAASKMRKAQDRMRAARP
                     YAEKVRNIAAHLGQANPEYVHPFMKANDAKAAGIIVVTTDKGLCGGMNTNVLRAVTGK
                     LRDLQTAGVQAQAVAIGNKGLGFLNRVGAKVVAQSVGLGDTPHLDKLIGPVKVLLDAY
                     AEGKINAVYLAYTKFINTMKQESVVEQLLPLSSEQLQANKSGHGWDYIYEPDAQSVID
                     ELLVRYVESLIYQAVAENMASEQSARMVAMKAATDNAGNVINELKLVYNKTRQAAITK
                     ELSEIVAGAAAV"
     gene            3547..4953
                     /gene="atpD"
                     /locus_tag="PK518_07330"
     CDS             3547..4953
                     /gene="atpD"
                     /locus_tag="PK518_07330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRM48765.1"
                     /translation="MAQVQGKIVQCIGAVVDVEFPRDQMPKVYDALKLEGSALTLEVQ
                     QQLGDGIVRTIALGSSDGLKRGIMVTNTGNPITVPVGKATLGRIMDVLGNPIDERGPV
                     SQELTASIHRKAPAYDELSPSQELLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNM
                     MELINNIAKAHSGLSVFAGVGERTREGNDFYHEMSDSKVVVQEDLSQSKVAMVYGQMN
                     EPPGNRLRVALTGLTIAESFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQ
                     PTLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFAHLDSTVVLSRDIA
                     ALGIYPAVDPLDSTSRQLDPHVVGEEHYQVARQVQGTLQRYKELRDIIAILGMDELAP
                     EDKLTVARARKIQRFLSQPFHVAEVFTGSPGKYVPLSETIRGFKMIAAGECDHLPEQA
                     FYMVGTIDEAFEKAKKLA"
     gene            5048..5464
                     /locus_tag="PK518_07335"
     CDS             5048..5464
                     /locus_tag="PK518_07335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRM48766.1"
                     /translation="MNTIHVDVVSAEESIFSGEARFVALPGEAGELGIYPRHTPLITR
                     IKPGSVRIELPDGNEEFVFVAGGILEVQPNCVTVLSDTAIRGRDLDDQKAQEAKASAE
                     EALKNAKSEIDLARAQSELAIMAAQIAALRKFRQKH"
     gene            complement(5566..6117)
                     /locus_tag="PK518_07340"
     CDS             complement(5566..6117)
                     /locus_tag="PK518_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRM48767.1"
                     /translation="MVSPTPNTPPSALRGLMRLLRHRWADGALHQTLPPDMLQRLAQR
                     ITASEQRHTGQIRICVEGGLPLSYIWRGAGARERAITQFGKLRVWDTEGNNGVLIYLL
                     LADHAIEIVADRGLTQHVAPQTWRTTVATMTEAFRAGHYEDGLTQALAEVSALLVAHF
                     PRDGFQTMDDSGNELPNDPVIAP"
     gene            complement(6143..7054)
                     /locus_tag="PK518_07345"
     CDS             complement(6143..7054)
                     /locus_tag="PK518_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRM48768.1"
                     /translation="MPIALAIQALIAILLIAIGIPPAGAQPLRAVPALNARVIDETGT
                     LGATELQSLQARLAAMEQRHGSQVVVLMVPTTAPEDIAAFANRVGNAWKIGRKEVGDG
                     VLIVVAKDDRRMRIEVAKALEGAIPDIAAARIIDGAMKPRFREGDFAGGLLAAVDQID
                     ARIAGESLPAPQGPGQGRGGQMQDEGSLSDLAIFLFFGVMVAGPVLRRLLGQRLGGLA
                     MGGGAGLLAFVVTSSLLLAAAAAVAALLYTWIFAGRNMPIVGHGGQHGGGRGGWGGGW
                     GSGGSPGGGFGSGGGGDFGGGGASGDW"
     gene            complement(7076..7681)
                     /locus_tag="PK518_07350"
     CDS             complement(7076..7681)
                     /locus_tag="PK518_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRM48769.1"
                     /translation="MQRLFAIIAAALLLSGCGYNDFQRLDEQSKAAWSEVLNQYQRRA
                     DLVPNIVATVKGEANFEQETLTKVIEARAKATSIQVTPETLNNPEAFNKFQQAQGELS
                     GALSRLMVVAERYPQLQANQAFRDLRVTLEGTENRITVARNRYIQSVQDYNVLARSFP
                     TNLTAMVFSYTAKPSFTVQNEAEISTPPKVDFSNPAPAVKP"
     gene            7822..8268
                     /locus_tag="PK518_07355"
     CDS             7822..8268
                     /locus_tag="PK518_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRM48770.1"
                     /translation="MTRPSLRAVLAGSPDELEAAFYDALQQGDLERLMACWADEDEIV
                     CLHPGGARLVGPAAIRAAYTAMLEHGGVQVRPAQVVRVQALASSVHSVLEQVRVVLPD
                     GAREALVNATNVYHKTPRGWRLVAHHASVGEAHEGGLPTSSAHMLH"
     gene            8278..8838
                     /locus_tag="PK518_07360"
     CDS             8278..8838
                     /locus_tag="PK518_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HRM48771.1"
                     /translation="MDDIVRQAIAKWPNVPHCYGWLGLDARGQWYLRDGATQALGGFD
                     VARGSQLQHAKLIDFIHRNYEADARGCWYFQNGPQRVYVELEAAPWVWRLHADGRVMS
                     HTGKVAEVREMRVDESGRVYLQTDIGPGLVHSMDVGLAADAVEQGRWVPIPIEGAVLA
                     QEFGFVRSPFALSQSRSQAGDPAPQA"
     gene            complement(8887..9714)
                     /locus_tag="PK518_07365"
     CDS             complement(8887..9714)
                     /locus_tag="PK518_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM48772.1"
                     /translation="MSDTHDEAHTGAIKNPKQFLTSTILAFVLPIFIIIGLVVFVTSG
                     SKPNAGVGNLEMAIAQRIQKVGNVEVRDANRPLRAGEAVYNGQCAACHATGAAGAPKL
                     GDAAAWGPRIKTGFEALVQSALKGKGAMAPQGGGDFNDTEIARGVAYMANAAGAKFVE
                     PAAPSGADAAAAAPTPAAAAPAAAPAAAAAPAAETDVAAGKKLYESTCVACHGAGVAG
                     APKFGDKAAWAPRLAAGFDEVLKIATTGKGAMPPKGGSSASDGEFKAAVEYLVNSAK"
     gene            complement(9824..11266)
                     /locus_tag="PK518_07370"
     CDS             complement(9824..11266)
                     /locus_tag="PK518_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRM48773.1"
                     /translation="MHKHQPPRTRAARALAPLAAIALLAGCSFIPSHERPAAPVPQAY
                     AQPGSEMPTTAKAAADIDWKEYFTDPRLQTLLGIALENNRDLRVAMLNVEQARAQFQI
                     QRANQYPTVNAIASGTRQPKTSGDGYSNLYQAGVGISAWEMDFFGRIAALKEQALAQY
                     LATAEARKSAQISLVAAVASGWLTLMADEELLDISRRTLQTREESVKLTRLRLEHGVS
                     SELDSHQAESLAQAARATYAQQLRQRQLDENALALLLGQSLPDAVRASLPSSRLADAA
                     PMQPLPPGLPSDLLERRPDIRQAEQMLIGANANIGAARAAFFPRIALTAQVGSVSSEL
                     SGLFKSGSWAFSLAPQLALPIFDAGRNQAGLEAAQAGRQIAVAQYEKSIQVAFREVSD
                     ALAGQATLQQQSEAQRAQVQADSKRLQLSDLRYRNGVASYLDLLDAQRSLFATEQALV
                     LTRLQQLQNQVTLYKVLGGGWTEATAATRS"
     gene            complement(11292..14444)
                     /locus_tag="PK518_07375"
     CDS             complement(11292..14444)
                     /locus_tag="PK518_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM48774.1"
                     /translation="MAKFFIDRPIFAWVIAIFIMVVGAVSITQLPVAQYPAVAPPTIQ
                     VSVAYPGATAQTLEDSVLSVIEREMNGATGLAYMETTSQANGTGTIVLSFEPGTDDSL
                     AQVDVQNRLSRATPRLPAAVTQQGVRVDKSRSNFLLFSILTSDNPDVSIEALNDYAAR
                     NVVPELQRLPGVGGVTQFGSERAMRIWIDPAKLKGFNLSLDQVNAAIRAQNVQVSAGN
                     LGDLPSPVGQTMSATIVVRGQLSSADEFGGIVLRANTDGSTVRLKDVARIELGSQSYS
                     TSAQLNGAPAVGMGVQLTPTANALATAEAVKAKLADLQRYFPQGVKYAIPYDTSTFIA
                     VSIEKVVHTLLEAVALVFLVMFLFLQNFRYTIIPTIVVPVALLGTFGALLAMGFSINV
                     LTMFGMVLVIGIVVDDAIVVVENVERIMSEEGLPPLQATRKAMGQVSGAIVGMTVVLV
                     SVFVPLAFFAGSTGNIYRQFAATMATSIAFSGFLALSLTPALCGTLLKPVTEGHHDKK
                     GFFGWFNRVFKSTTQRYESGLSRLARRSGRMMIIYAALIGVVGIVYLRLPTSFLPNED
                     QGYVIANVQLPAGASMERTRNVLNQVEAFMLKQPEVSNIVSVAGFSFSGQGQNTGIAF
                     IPLKNWSERPNPDQSAQALAGRAMGALGGIRDAFIFVLSPPPIPELGSASGFTFRLQD
                     RGAKGHDALLAARNQLLGMVAQSKVLAGVRPDGMEDAPQMQIDIDRDKANALGVSFDR
                     INGALATALGSTYINDFPNQGRLQRVVVQADAQARMQPESVLEIPVLNDKGQVLPLST
                     FASTRWITGAMQTVRYNGYPSMKIAGDAAPGYSTGEAMQEMERLAGQLPEGFGFEWTG
                     QSREEKLAGGQAMVLYAFSLLAVFLCLAALYESWSIPFSVMMVVPLGVLGVVLGVLLR
                     GMANDVYFQIGLVTIIGLSAKNAILIVEFAKDLQAQGKSVIDAALEAAHLRFRPILMT
                     SLAFTLGVLPLFFASGASSASQRAIGTGVVGGMITGTVLAVIFVPLFFVLIRGFFKGS
                     QRQRDHDSKQALKHNSESAT"
     gene            complement(14469..15740)
                     /locus_tag="PK518_07380"
     CDS             complement(14469..15740)
                     /locus_tag="PK518_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM48775.1"
                     /translation="MSPLPQRRQPHRTSLQPHPTAAWSLALTAVLLLTACGKPEAQPG
                     GGGGKPPAPEVGVLTVQPGEIGLITELPGRLEASRVAQVRARAAGILQKRVFNEGSDV
                     KAGQKLFLIDSAPYQASLAQAEASVAQAEASLAQSRALAERYRPLVAVNAISKQEYDN
                     AVAAQKMAQANVAAAKASVTTARINLGYATVTAPISGRIGRALVTEGALVGQGTPTEL
                     AMIQQIDPLYINFTQSASDALKLRAAVASGKYKQAGANAAHVRVVLEDGSIYPQTGRL
                     LFADLTVDPTSGQVSLRAEIPNHDRSLLPGLYVRVRLEQAQVDNGILLPQQAVTRSGK
                     TDTVMVVGADGHVAPRVVKLGPAQGSNWVVLDGLQSGEQVMVDGFQKLPRGKPGDPIV
                     VKPVAWQAPGAPKAPAAPSSAASPPAAPAKQ"
     gene            complement(15890..16927)
                     /locus_tag="PK518_07385"
     CDS             complement(15890..16927)
                     /locus_tag="PK518_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRM48776.1"
                     /translation="MDKEDARKQTLEQLYERRKQVVRLHKRGMGVMQIVAMTGLSYPT
                     VRSCIDRFDAGGWAAIRPARRGRQRGAGRVLTPAQEQAIQRMIIDHRPEQLKMDFFLW
                     SRAAVAELIQCEYGIRLQPRSVGKYLARWGFTPQRPLKRAYEQKPEAVQAWLQGEYPG
                     IEQRARAEGAEIHWGDETALVNTDVRGRSFAPRGKTPVLPTVGGTRQKLSMIATVTNQ
                     GKTRWMIIDEAFNADKLIEFLQALIQDAGRKVFLILDNLRVHHSKIVKAWVAERKEQI
                     ELFYLPSYSPQLNPEERLNADLKQEMGKRVPVRTKAKLREAASAHMRMLDNNPERVIS
                     YFQDPRVRYAA"
BASE COUNT         3036 a         5305 c         5597 g         3099 t
ORIGIN      
        1 agaaaggtgt tgccgtgagt atcaacgcga ccctgttcgt tcaggccata gtctttctga
       61 ttctggtgtg gttcacgatg accttcgtgt ggcccccgat cgccaaggct ctggatgagc
      121 gagcccagaa aatcgccgat ggtctcgctg cggctgacaa ggccaagacc gaactcgccg
      181 ctgccgacca gcgcgtgaag aaggaattgg ctgctgccag caacgaaacc gccacgcgcc
      241 tggctgacgc cgagcgccgc gcccaggcca tcatcgaaga ggccaaggca cgcgccaccg
      301 acgaaggcaa caagatcgtc gccgccgccc gtgccgaggc cgagcagcag gccattgccg
      361 cccgtgagac cctgcgcgag caggtggccg cactggccgt caagggtgcc gagcagattc
      421 tccgcaagga agtcaatgcc ggcgtccatg ctgatctgct caaccgcctc aagaccgagc
      481 tgtaaggaac gcaacatggc tgaactcgcc accattgccc gcccttacgc cgaggcgcta
      541 tacaaggcct gcaccggcaa aaccggtgtc gatcttggcg gtgccagcgc ctgggtggac
      601 gagctggcgg cgattgccgc caacccacaa ttgcgccagc tgctggacaa ccccaaggtg
      661 tcggccgaac agttgttcgg cctcatcacc ggtgtggccc gcacggcgtt gccggacatg
      721 gcgcacaact tcctgcgcac cctgcttgac aacggccgtg tgcaggcgct gcccgagatt
      781 gcggcacagt ttcgcgcgct ggtgaaccaa ggcaatggct cttcggatgc cgtcgtctac
      841 agcgccttcc cgctggatgc caacgcactg gccgagctcg gcgccacgct gcaaaagcgc
      901 tttggccgcc agctgaacct ctccgtgcag acggacgagt cgctcatcgg cggcgtgcgt
      961 gtggtggtgg gcgacgaggt gctcgacacc tccgtcaagg cacgtctgga acaaatgaaa
     1021 gcggccctca tcgcctgatc gggtgctgag gtctgggctt attaaagaaa gaaggaaaga
     1081 gtcatgcaac tcaatcccgc agaaatttct gaactgatca agagccgcat tgaagggctg
     1141 gcagccagca gcgacatccg caaccagggc accgtggtgt ccgtgaccga cggtatcgtg
     1201 cgcatccatg gcctgtcgga cgtgatgcag ggcgaaatgc tggaatttcc cgccgacgcg
     1261 gacggccagc ccagctacgg cctggcgctg aacctcgagc gcgactccgt gggctccgtg
     1321 attctgggcg gctacgagca tatctccgaa ggcgataccg tcaagtgcac gggccgcatt
     1381 ctggaagtgc ccgtgggccc cgaactggtc ggccgcgtgg tcaacgccct gggtcagccc
     1441 atcgacggca agggcccgat caacgccaag atgacggacg tgatcgaaaa ggtcgctccg
     1501 ggcgtgatcg cacgccagtc cgtggaccag cccctggcca ccggccagaa gtccatcgat
     1561 tccatggtgc ccgtcggccg cggccagcgc gagctgatca tcggcgaccg ccagaccggc
     1621 aagacggctg tggccatcga tgccatcatc gcccagaagg gccagggcgt gacctgcatc
     1681 tacgtcgcca tcggtcagaa ggcttcgtcg atcaagaacg tggtgcgcgc gctggagcag
     1741 gccggcgcca tggaatacac catcgtcgtc gccgcctcgg cttctgaatc ggctgccatg
     1801 cagtatgtgt cggcctactc cggctgcacc atgggcgagt acttccgcga ccgcggccag
     1861 gatgcgttga tcgtctatga cgatctgtcc aagcaggccg tggcctaccg ccaggtctcg
     1921 ctgttgctgc gccgcccgcc gggccgcgaa gccttccctg gcgacgtttt ctatctccac
     1981 agccgcctgc tcgagcgcgc cgcgcgcgtg aacgccgact acgtcgaagc cttcaccaag
     2041 ggtgaagtca agggcaagac cggttcgctg accgcactgc cgatcatcga aacgcaagcc
     2101 ggcgacgtgt ccgccttcgt gccgaccaac gtgatctcga tcaccgacgg ccagatcttc
     2161 ctggaaacca gcctgttcaa cgccggtatc cgccccgcca tcaacgccgg tatctcggtg
     2221 tcgcgcgtcg gtggtgccgc ccagaccaag ctgatcaagg gcctgtccgg cggtatccgt
     2281 accgacctgg cgcagtaccg cgaactggcc gccttcgcgc agtttgcctc tgacctggac
     2341 gaggccaccc gcaagcagct ggaccgcggc gcgcgcgtca ccgagctcct gaagcagccc
     2401 cagtacagcc cgctgcccat cagcctgatg ggttcctcgc tgttcgctgt gaacaagggc
     2461 ttcctggacg atgtggacgt gaagaaggtg ctgtccttcg aatccggcct gcaccagttc
     2521 ctgaagacca gccacgcggc cctgctggag cgcctggaag ccaaccgtgc cttcgacaag
     2581 gaaggcaagg acgaagccga gctgacgcaa gccatcactg cgttcaagaa gaccttcgcc
     2641 taacgcggca ggaggagtca tcatggcagc aggcaaggaa atacgcggca agatcaaatc
     2701 ggtggagaac accaagaaga tcaccaaggc catggaaatg gtggccgcgt ccaagatgcg
     2761 caaggcgcag gatcgcatgc gcgccgcccg tccctatgcc gagaaggtgc gcaacattgc
     2821 ggcccatctc ggtcaggcca accccgagta cgttcaccct ttcatgaagg cgaacgatgc
     2881 aaaggcggcc ggcatcatcg tggtcaccac ggacaagggg ctgtgcggcg gcatgaatac
     2941 caacgtgctg cgtgccgtca ccggcaagct gcgtgacctg cagactgcag gtgtgcaggc
     3001 gcaggcggtg gccattggca acaagggcct gggttttctg aaccgcgtgg gcgccaaggt
     3061 ggttgcgcag tccgttggtc tgggcgacac gccgcatctg gataagctca tcggcccggt
     3121 caaggtgctg ctggacgctt atgccgaagg caagatcaat gcggtgtacc tggcgtacac
     3181 caaattcatc aacaccatga agcaggaatc ggtggtggag cagttgctgc ccctgtcctc
     3241 cgagcagctg caggccaaca agtccggtca cggttgggac tacatctatg agcccgacgc
     3301 tcagagcgtg attgacgagc tgctggtgcg ttatgtcgag tcgctgatct accaggccgt
     3361 ggcagagaac atggcttcgg agcaatcggc gcgcatggtg gccatgaagg ccgccaccga
     3421 caacgcgggc aacgtcatca acgagctcaa gctggtctac aacaagacgc gccaggccgc
     3481 gatcacgaaa gaactttcgg agatcgtcgc cggtgcggcg gcagtttaaa tttctggagc
     3541 aaacaaatgg ctcaagtgca aggcaagatt gttcaatgta ttggcgccgt ggtggacgtg
     3601 gagttcccgc gcgaccagat gcccaaggtg tatgacgccc tcaagctcga gggctcggcc
     3661 ctgacgctgg aagtgcagca gcagctcggt gacggcatcg tgcgcaccat tgccctcggc
     3721 tcgtccgacg gcctgaagcg cggcatcatg gtgaccaata ccggcaaccc catcaccgtg
     3781 cccgtgggca aggcgacgct gggccgcatc atggacgtgc tgggtaaccc catcgacgag
     3841 cgcggccccg tcagccagga gctgacggcc tccatccacc gcaaggctcc cgcctacgac
     3901 gaactgtcgc cgtcgcaaga gctgctggaa accggcatca aggtgatcga cctggtgtgc
     3961 ccgttcgcca agggcggcaa ggtgggcctg ttcggtggcg ccggcgtggg caagaccgtg
     4021 aacatgatgg agctcatcaa caacatcgcc aaggcgcact cgggtctgtc cgtgtttgcc
     4081 ggcgtgggtg agcgtacccg tgagggcaac gacttctatc acgaaatgtc cgactccaag
     4141 gtcgtggtgc aggaagacct cagccagtcc aaggtggcca tggtctacgg ccagatgaac
     4201 gagcccccgg gcaaccgtct gcgcgtggcg ctgacgggtc tgaccatcgc cgagtccttc
     4261 cgtgacgaag gccgtgacgt gctgttcttc gtggacaaca tctaccgcta caccctggcc
     4321 ggtaccgaag tgtccgccct gctgggccgt atgccttccg ccgtgggcta ccagccgacg
     4381 ctggccgagg aaatgggccg cctgcaggag cgcatcacct ccaccaaggt cggctcgatc
     4441 acctccatcc aggccgtgta cgtgcctgcc gacgacttga ccgacccgtc gcctgccacc
     4501 accttcgccc acctggactc caccgtggtg ctgtcgcgtg acatcgcagc cctgggtatc
     4561 tacccggccg tggatccgct cgattccacc agccgccagc tcgaccccca tgtggtgggc
     4621 gaggagcact accaggttgc ccgccaggtg cagggcacgc tgcagcgcta caaggaactg
     4681 cgcgacatca tcgcgattct gggtatggac gagctggcgc cggaagacaa gctgacggtg
     4741 gcccgcgcgc gcaagatcca gcgtttcctg tcgcagccct tccacgtggc cgaggtgttc
     4801 acgggctcgc ccggcaagta tgtgcccctg tccgagacca ttcgtggctt caagatgatt
     4861 gcggccggtg agtgcgatca cctgcctgag caggccttct acatggtcgg caccatcgac
     4921 gaagccttcg agaaggccaa gaagctggcg tgatgtgaag ttgagcgttg agcgttgagg
     4981 gtcgagcgtt aagccgtcct tgggtacgcc aacgcacaac acacaacgca caaccgaagg
     5041 agttttcatg aacaccatcc acgttgatgt ggtcagtgcc gaagagtcca tcttctccgg
     5101 cgaggcacgc tttgtcgcct tgcccggcga ggcgggcgaa ctgggcatct acccgcgcca
     5161 cacaccgctg attacccgca tcaagccggg gtcggtgcgt atcgagctgc ccgatggcaa
     5221 cgaagagttt gttttcgtgg ccggtggcat cctggaagta cagcccaact gcgtgaccgt
     5281 attgtccgat accgccattc gcggccgcga cctggatgac cagaaggcac aagaggccaa
     5341 ggcctcggcc gaagaagcgc tgaaaaacgc gaagagcgaa atcgacctgg cgcgtgccca
     5401 gtccgagctc gccatcatgg cggcacagat cgcggcgctg cgcaagttcc gtcagaagca
     5461 ctgacaagcc gggccgtcaa ggctctggct gccaacctaa aggaacgcct gccgcgttcc
     5521 tttttttatg tcttagcgcg ttgcgacacg gatgtcgctt agctctcagg gcgcgattac
     5581 cgggtcgttc ggcaactcgt tgccgctgtc atccatagtc tgaaagccat cccgggggaa
     5641 atgcgcgacc aatagcgccg aaacctccgc cagcgcctgg gtcagtccat cctcatagtg
     5701 tcccgcgcga aaggcctcgg tcatggtggc caccgtcgtg cgccaggttt gcggggcaac
     5761 atgctgggtg aggccgcgat cggcaacgat ctcgatggcg tgatcggcca acagcagata
     5821 gatcagcacg ccgttgttgc cctccgtgtc ccatacgcgc agcttgccga actgggtgat
     5881 ggcgcgctcg cgcgcgccgg cgccgcgcca gatgtaggac aggggcaggc cgccctcgac
     5941 gcaaatgcgg atctgcccgg tgtggcgctg ttcgctggcg gtgatgcgtt gcgcgaggcg
     6001 ttgcagcatg tcaggtggca gggtctggtg cagggcaccg tcggcccagc ggtggcgtag
     6061 cagccgcatc aagccgcgca gtgcagaagg tggcgtgttt ggcgtggggg agaccatggt
     6121 gattgttaag tccttgtctt gtctaccagt cgcccgaggc accgccaccg ccaaaatcac
     6181 cgccgccgcc ggagccaaag ccgccgcccg ggctgccgcc gctgccccag ccaccccccc
     6241 agccgccacg gccaccgccg tgctggccgc catgacctac gatgggcatg ttgcgcccgg
     6301 cgaagatcca ggtgtagagc agggccgcga cggctgccgc ggccgccagc agcaggctgg
     6361 aggtgacaac aaaggccagc aggcctgcgc cgccgcccat ggccaggccg cccaggcgtt
     6421 gccccaacag gcgccgcaac acggggccgg ccaccatgac accaaagaac aggaagatgg
     6481 ccagatcgga caaactgcct tcgtcctgca tctggccacc ccttccctgg ccggggccct
     6541 gcggagcggg gaggctttcg ccggcgatgc gtgcgtctat ctgatccact gccgccagca
     6601 ggccaccggc gaagtccccc tcgcgaaagc gtggcttcat ggcgccgtcg atgatgcggg
     6661 cggcggcaat gtcggggatg gctccctcca gcgccttggc cacttcgatg cgcatgcgcc
     6721 tgtcgtcctt ggcgacgacg atcagcacgc cgtcgcccac ctccttgcgc ccgatcttcc
     6781 aggcattgcc gacgcggttg gcaaaggcgg caatgtcctc aggcgccgtg gtgggcacca
     6841 tcagcaccac cacctgagag ccatggcgct gttccatggc cgccagtcgg gcctgcagcg
     6901 actgcaactc ggttgcgccg agcgtgcctg tctcgtcaat cacgcgcgcg ttcagcgcgg
     6961 ggacggcgcg cagtggctgg gcgccagccg gaggaatgcc gattgctatt aataatatag
     7021 ctatcagcgc ttgaatggca agcgctatag gcatatttag ctcagattga tatggtcagg
     7081 gcttgaccgc cggagcgggg ttggagaagt ccaccttggg tggggtgctg atctcggctt
     7141 cgttctgcac cgtgaagctg ggcttggcgg tatagctgaa taccattgcc gtgaggttgg
     7201 tggggaagct gcgcgcgagc acgttgtagt cctgaaccga ctggatatag cggttgcgtg
     7261 ccacggtgat gcggttttcc gtgccctcca gggtcacgcg caaatcacgg aaggcctggt
     7321 tggcctggag ctgcgggtag cgctcggcca ccaccatcag gcgtgatagc gcgccggaca
     7381 gctcgccctg ggcctgctgg aacttgttga acgcctcggg gttgttcagg gtctcaggcg
     7441 tgacctggat cgatgtggcc ttggcgcgcg cctcgatcac cttggtgaga gtctcctgct
     7501 cgaaattggc ctcgcccttc accgttgcca cgatattggg taccaggtcg gcgcgccgtt
     7561 ggtactggtt cagcacctcg ctccatgcgg ccttggactg ctcgtccagg cgctggaagt
     7621 cgttgtagcc acaaccggac aaaagcagtg cggcggcaat gatggcaaag agtctttgca
     7681 tgatttcaac ggtgttggtg atggggtggg cgcagtggcg gcgcaggggg tgccggcatt
     7741 gtgcatgtat tggcgcaacc cgtgacaaga ggcgccctgg tgccgacaat agttgacccg
     7801 aatgttcttc tccattgcct gatgaccaga ccctccttgc gtgccgtatt ggccggcagt
     7861 cccgatgagc tcgaagccgc gttctatgac gccctgcagc agggcgacct ggagcgcctg
     7921 atggcctgct gggccgacga ggatgaaatt gtctgcctgc acccgggtgg cgcccgcctc
     7981 gtgggtccgg ctgcgatacg cgcggcctac accgccatgc tggagcatgg cggcgtgcag
     8041 gtacgaccgg cacaggtggt gcgggtgcag gccctggcca gcagtgtgca cagcgtgctg
     8101 gagcaggtgc gcgtggtgct gccggacggc gcgcgcgagg cgctggtcaa cgccaccaac
     8161 gtctatcaca agacacccag gggctggcgc ctggtggcgc atcatgccag tgtcggagag
     8221 gcgcatgagg gcggcctgcc cacctcgtcg gcgcatatgc tgcattgagg cgcgaccatg
     8281 gatgacatcg tcaggcaggc catcgccaaa tggcccaacg tgccgcactg ctatggctgg
     8341 ctggggctgg atgcacgcgg ccagtggtat ctgcgcgatg gtgcaaccca ggctctgggc
     8401 ggctttgatg tggcgcgcgg cagccaactg cagcatgcca agctgatcga cttcatacac
     8461 cgcaactatg aggccgatgc gcgcggttgc tggtactttc agaatggacc gcagcgagtc
     8521 tacgtagaac tggaggcggc accctgggtc tggcgcctgc atgccgatgg cagggtgatg
     8581 tcgcacaccg gcaaagttgc tgaggttcgg gagatgcggg tggatgagtc tggccgggtg
     8641 tatttgcaga ccgacatcgg ccctggcctg gtgcactcga tggatgtggg actggctgcc
     8701 gatgcggtgg agcaggggcg ctgggtaccg attccgatag agggagcggt gcttgcgcag
     8761 gagttcggct ttgttcgcag tccgttcgcg ctcagccaga gcagatccca agccggcgac
     8821 ccagcgccgc aggcatgaaa aaagccgatc aaggatcggc ttttttgtga tctgtctgtg
     8881 gcgcgcttac ttggcggaat tcaccaggta ttcgacagcg gccttgaact ccccatccga
     8941 ggcgctggag ccgcccttgg gaggcatggc gcccttgccg gtggtggcga tcttcagcac
     9001 ctcgtcaaag ccggcggcaa ggcggggcgc ccaggcggcc ttgtcgccga acttcggcgc
     9061 gcctgcaacc cctgcgccat ggcaggccac gcaggtggat tcatagagct tcttgccggc
     9121 tgcgacgtcg gtttctgcgg ctggtgcggc ggcggctgcc ggagctgctg cgggggcggc
     9181 cgctgcagga gtgggcgcag cggcggcggc gtcggcgccg ctcggtgccg caggttcaac
     9241 gaacttggcg cccgcggcat tcgccatgta ggccacgccg cgcgcgatct cggtgtcgtt
     9301 gaagtcgccg ccgccttgcg gtgccatggc ccccttgccc ttgagtgcgg actgcaccag
     9361 agcctcgaag ccggtcttga tgcgcggacc ccaggcggcc gcatcgccca gcttgggagc
     9421 gccggcggcg cccgtggcat ggcatgcggc gcactggccg ttgtacaccg cttcgcccgc
     9481 acgcagcgga cggttggcgt cgcgcacctc cacgttgccg accttttgaa tgcgctgcgc
     9541 aatcgccatc tcgaggttgc ccacgccggc gttgggtttg ctgcccgagg tcacaaacac
     9601 caccaggccg atgatgatga agatcggcaa cacgaaggcc aggatggtgg aggtcaggaa
     9661 ctgcttcggg tttttgatgg cgccggtatg cgcttcgtca tgggtgtcgc tcatggaatc
     9721 ctcgggtgat gtttttgtgc tggcggcgga aggaaaatca ggcgccgatt ataggagtcc
     9781 accttgcgcc acgcccgcct gcgggaaacg ggcgggcacc ccatcaggag cgggttgcgg
     9841 cggtggcttc cgtccagccg ccgcccagca ccttgtacag cgtgacctgg ttttgcagct
     9901 gctgcaacct tgtcagcacc agcgcctgtt cggtggcgaa cagtgagcgc tgcgcgtcca
     9961 gcagatccag gtagctggcg acaccgttgc ggtagcgcag gtcggacagc tgcaggcgct
    10021 tgctgtcggc ctgtacctgg gcgcgctggg cctcgctttg ctgctgcagc gtggcctggc
    10081 cagcgagcgc atccgacacc tcgcggaagg ccacctgtat cgatttttcg tactgggcaa
    10141 cggcgatctg gcgcccggcc tgcgcggcct ccagcccggc ctggttgcgg ccggcatcga
    10201 agatcggcag agccagttgc ggcgccagcg agaaggccca ggagccgctc ttgaacaggc
    10261 cggagagctc gctgctcacg ctgcccacct gcgcggtgag ggcgatgcgc ggaaagaagg
    10321 ccgcgcgtgc cgcgccgatg ttggcattgg cgcctatcag catttgctcg gcctggcgga
    10381 tgtcggggcg gcgctccagc agatccgaag gcaggcccgg gggcagcggt tgcatcgggg
    10441 cggcatcggc cagacgtgac gagggcaggc tggcgcggac cgcgtcgggc aggctctggc
    10501 ccagcagcag ggccagggcg ttttcgtcca gttggcgctg gcgcagttgc tgcgcgtagg
    10561 tggcacgggc cgcctgggcc aggctctcgg cctggtggct gtccagttcg gagctcacgc
    10621 catgctccag gcgcaggcgt gtcagcttga cggactcttc gcgcgtctgc agcgtgcggc
    10681 gcgaaatatc cagcaactcc tcgtcagcca tgagtgtcag ccagcccgag gccacggccg
    10741 cgaccaggct gatctgtgct gatttgcgcg cctcggctgt ggccaggtac tgggccaggg
    10801 cctgctcctt gagggcagca atgcgcccga agaaatccat ctcccaggcc gagatgccca
    10861 ctcccgcctg atacagattg ctgtagccgt cgcccgaggt cttgggttgg cgcgtgccgc
    10921 tggcgatcgc attcaccgtc gggtattggt tggcacgctg gatctggaac tgcgcgcgcg
    10981 cctgctccac gttcagcatg gccacgcgca ggtcgcggtt gttctccagg gcgatgccca
    11041 gcagggtttg caggcgcggg tcggtgaagt attccttcca gtcgatgtcg gccgccgcct
    11101 tggctgtggt gggcatctca ctgccgggct gggcataggc ctgaggcacg ggtgccgccg
    11161 gccgctcatg gctggggatg aagctgcagc ctgccagcaa tgcgatggct gccagcggtg
    11221 ccagggcgcg cgcggcgcgc gtgcggggcg gttggtgctt gtgcatgatg ttagttactc
    11281 cggcggcggt atcaggttgc gctttcgctg ttgtgtttca gcgcttgctt gctgtcatgg
    11341 tcgcgctggc gctggctgcc cttgaagaag ccgcggatca gtacaaagaa cagcgggaca
    11401 aagatcactg cgagtacggt gccggtgatc atccctccga ccacaccggt gccgatggcg
    11461 cgttggctgg ccgagctggc gcccgatgcg aaaaacaacg gcagcacgcc cagcgtaaag
    11521 gctagcgaag tcatgaggat ggggcggaag cgcaggtggg ccgcctccag cgccgcgtcg
    11581 atgacgctct tgccctgggc ttgcagatcc ttggcgaact cgacgatcag gatggcgttc
    11641 tttgcggaca ggccgatgat ggtcaccagc ccgatctgga agtacacgtc gttggccatg
    11701 ccgcgcagca gcacacccag tacgacgccc agcacgccca gtggcaccac catcatcacc
    11761 gagaacggga tggaccagct ctcgtacaga gccgcaagac acaggaatac ggccagcaat
    11821 gagaatgcat acagcaccat agcctggccg ccggcgagct tttcttcgcg cgactggccc
    11881 gtccactcga agccaaagcc ttcgggcaac tggccagcta ggcgctccat ttcctgcatc
    11941 gcctcgccgg tgctgtagcc gggtgcggcg tcgccggcaa tcttcatcga cggataaccg
    12001 ttgtagcgca cggtttgcat ggcgcctgtg atccagcgtg tggaagcaaa ggtggatagc
    12061 ggcagcactt gccccttgtc gttgagcacc gggatttcca gcaccgactc gggttgcatg
    12121 cgtgcctgcg catcggcttg caccaccaca cgttgcaggc ggccctggtt cgggaagtcg
    12181 ttgatgtagg tggatcccag cgccgttgcc agtgcgccgt tgatgcggtc aaacgatacg
    12241 cccagggcgt tggccttgtc gcggtcgatg tcgatttgca tctggggtgc atcctccatg
    12301 ccatcgggac gcacgccggc cagtaccttg ctttgtgcca ccatgccgag caattggttg
    12361 cgcgcggcca gcagggcgtc atggcccttg gcgccacgat cctgcaggcg gaaggtgaac
    12421 cccgaggccg aacccagctc gggaatgggc ggcgggctca agacgaagat gaatgcgtcg
    12481 cgaatgcctc cgagcgcacc catggcacgg ccggcgagcg cctgcgccga ttggtcgggg
    12541 ttggggcgct cgctccagtt cttgagtggt atgaaggcaa tgcctgtgtt ctgaccctgg
    12601 cccgagaatg agaagccggc cacggagacg atgttgctca cctcgggctg cttgagcatg
    12661 aaggcttcga cctggttcaa gacgttgcgt gtgcgctcca tggacgcgcc tgcgggcaac
    12721 tgcacgttgg cgatcacata gccctgatcc tcattgggca ggaaggaggt gggcaggcgc
    12781 aggtacacga tgcccacgac gccgatcagc gccgcataga tgatcatcat gcgtccgctg
    12841 cggcgtgcca ggcgggacag gccggactca tagcgctgcg tggtgctctt gaacacgcgg
    12901 ttgaaccagc caaagaagcc ctttttgtca tgatgcccct cggtcacggg cttgagcaag
    12961 gttccgcaca gcgccggtgt gagcgacaag gcgaggaagc cggagaacgc gatcgaggtg
    13021 gccatggtgg ccgcgaactg gcggtaaatg ttgcctgtgg agccggcgaa gaaggccagc
    13081 ggcacgaaca ccgagaccaa caccacggtc atgccgacaa tggcgccgga gacttggccc
    13141 atggccttgc gcgtggcctg cagcggcggc agtccttcct cggacatgat gcgctccacg
    13201 ttttccacca ccacgatggc gtcatccacc acgatgccga tcaccagcac catgccgaac
    13261 atggtcagca cgttgattga aaaacccatg gccagcagcg cgccgaaggt gcccagcagg
    13321 gccacgggca ccacgatggt cgggatgatg gtgtagcgga agttctgcaa gaacaggaac
    13381 atcaccagga acaccagcgc cacggcttcg agcagcgtat gcacgacctt ctcgatggat
    13441 acggcgatga aggtggaggt gtcgtaggga atggcgtatt tcacgccctg cgggaaatac
    13501 cgttgcaaat cggccagctt ggccttcacg gcctcggccg tggccagggc gttggccgtg
    13561 ggggtgagct gtacgcccat gcccaccgca ggggcgccat tgagctgcgc gctggtactg
    13621 tagctttgcg agcccagttc gatgcgtgcc acatccttga ggcgtacggt ggagccgtcg
    13681 gtgttggcgc gtagcacgat gccaccaaat tcgtcagcgg aggacagttg tcccctcacc
    13741 acgatggtgg ccgacatggt ctgacccacg gggctgggca ggtcgccaag attgcctgca
    13801 gacacctgga cgttttgtgc gcggatggcc gcgttgactt ggtctaacga gagattgaag
    13861 cccttgagct tggccgggtc gatccagatg cgcatggcgc gctcggagcc gaactgggtc
    13921 acaccgccca cgcccggcag gcgctgcagt tcgggtacga cgttgcgtgc cgcgtagtcg
    13981 ttcagtgcct cgatgctgac atccgggttg tcggaggtga gaatggaaaa cagcagaaag
    14041 ttggaacgtg acttatccac gcgcacgccc tgctgcgtga cggcggcggg caggcgcggc
    14101 gtggcgcgcg agaggcggtt ttgcacgtcc acctgcgcca ggctgtcgtc ggtgccgggc
    14161 tcgaagctca gcacgatggt gcccgtgccg ttggcctgac ttgtggtttc catgtaggcc
    14221 aggccggtgg caccgttcat ctcccgctcg atgacggaga gcacggagtc ctcaagggtt
    14281 tgtgcagtgg caccggggta ggcgacagac acctggatgg tgggtggtgc tacagcggga
    14341 tactgcgcaa cgggtaactg ggtgatcgac accgcaccca ccaccatgat gaagatggcg
    14401 atcacccacg cgaagatagg gcgatcaatg aagaatttgg ccatgtgccg ggctcctatt
    14461 tcttgttttt attgcttggc tggggcggcg ggcgggctgg cagcggatga gggagcggcc
    14521 ggagccttgg gcgcgccagg ggcctgccag gctacgggtt tcacgacaat cgggtcacca
    14581 ggcttgccgc gtggcagctt ctgaaagccg tccaccatga cctgctcgcc agactgcagg
    14641 ccatcgagta ccacccagtt cgatccctgg gccgggccga gcttgaccac gcgcggcgcg
    14701 acatggccat ctgctcccac caccatcacc gtgtcggtct tgccggagcg ggtaacagcc
    14761 tgctgcggca gcaaaatgcc gttgtcgacc tgcgcctgct ccaggcgcac gcgcacgtaa
    14821 aggccgggta gcaaagagcg gtcgtggttc ggtatctcgg cgcgcaggct gacctggccg
    14881 ctggttggat ccaccgtgag atcggcaaac agcaggcgtc ccgtctgtgg gtagatgctg
    14941 ccgtcttcca gcaccacgcg cacatgggca gcattggcac cggcctgttt gtactttccg
    15001 ctggccacgg cagcgcgcag cttgagtgcg tcggaggccg actgcgtgaa gttgatgtag
    15061 agcggatcga tttgctggat catggccagc tcggtcggcg taccctggcc gaccagcgcc
    15121 ccttcagtca ccagggcgcg gccaatgcgg ccggagatgg gcgccgtgac cgtggcgtaa
    15181 cccaggttga tgcgggccgt ggtcacgctt gctttggccg cggccacatt ggcctgggcc
    15241 atcttttgtg cggccacggc gttgtcgtat tcctgtttgc tgatggcatt gaccgccacc
    15301 agggggcgat aacgctcggc cagcgcacgg ctttgggcca ggctggcctc ggcctgggcg
    15361 acgctggcct cggcctgcgc caggcttgcc tggtagggcg cgctgtcgat caggaacaac
    15421 ttctggccag ccttcacatc gctgccttcg ttgaaaaccc gcttctgcag gatgcccgcc
    15481 gcccgtgcac gtacctgtgc cacgcgcgat gcttccaatc tgccgggcag ttcggtgatc
    15541 aacccaattt cacccggctg caccgtgaga acgccaacct caggggccgg cggcttgcca
    15601 cctccgccgc cgggctgcgc ctcgggcttg ccgcatgcag tcaatagcag tacggcagtc
    15661 agggccagcg accaggccgc cgtggggtgg ggttgcaggc ttgtgcgatg gggctggcgg
    15721 cgttgtggca gaggtgacat gaagatcctt tggctggctg atggcaggtg cgcagggtgg
    15781 agcgcagggc gatatggccg tccagtcatt ttacgaaatc ggaaattata catacatttg
    15841 tgaatgtata attacacaaa actgcttatt gatccggccc aatgaagttt taagcggcat
    15901 agcgcacgcg agggtcttga aagtagctga tcacacgctc aggattgttg tccagcatcc
    15961 gcatatgcgc actggctgcc tcgcgtaact tggccttggt gcgcaccggc acacgcttgc
    16021 ccatttcctg cttcaaatcc gcgttcagcc gctcctcggg attgagctgt gggctgtagc
    16081 tgggtagata gaacagctcg atttgttcct tgcgctcggc cacccaggcc ttgacgatct
    16141 tgctgtggtg cacccgcagg ttgtccagaa tcaggaacac tttgcggccc gcatcctgaa
    16201 tcaaggcttg caagaactcg atcagcttgt cggcattgaa tgcttcatca atgatcatcc
    16261 agcgcgtctt gccctgattg gtgacggtgg cgatcatgga cagcttctgg cgtgtgccgc
    16321 ctacggtggg cagcaccggc gttttgcctc tgggcgcaaa gctgcgcccg cgcacgtcgg
    16381 tgttcaccag cgctgtctca tcgccccaat gaatctcggc gccctcggcg cgggcacgct
    16441 gctcgatgcc cggatactcg ccttgcaacc acgcttgcac cgcctcgggc ttttgctcgt
    16501 aggcgcgctt gagggggcgc tggggcgtaa aaccccagcg cgccaggtac ttgcccacgc
    16561 tgcgcggttg caggcgaatg ccgtattcgc actggatgag ctcggccacc gcagcgcgac
    16621 tccacaggaa gaagtccatc ttcaattgct cgggacggtg atcgatgatc atgcgctgta
    16681 tggcttgctc ctgggcgggt gtgagcacac ggccagcacc gcgctggcgt ccacgtcgcg
    16741 caggtctgat ggccgcccag ccgcccgcat cgaaccgatc tatgcagctc ctgaccgtgg
    16801 ggtaactgag ccccgtcatg gccacgatct gcatcacgcc catgccccgc ttatgcaacc
    16861 gcacgacctg tttgcgcctt tcgtacaact gctccagggt ttgctttctt gcgtcttctt
    16921 tgtccatgtc acatcggact cagtcataca cggaaagttc atacttcatt gggccgaatc
    16981 aataaaaaag gagatgtaag catggcgcgt cgcaccaaag tagacgcaga tgcgacc
//
LOCUS       DAOMTQ010000056        16971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_487_length_16971_cov_29.763892, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000056 DAOMTQ010000000
VERSION     DAOMTQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 16971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16971
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_16971_cov_29.763892"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK518_07390"
     CDS             complement(<1..314)
                     /locus_tag="PK518_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48777.1"
                     /translation="MHLQPYQSVDGTPFTATREDIIRAHGPPHSEERNNIGLTALDYG
                     ALVYRFQDSGRLEEVTARAQVLHLGALAIPLGTLAMFVRANDSAAFERAGFLVSPRYG
                     IA"
     gene            545..1753
                     /gene="trpB"
                     /locus_tag="PK518_07395"
     CDS             545..1753
                     /gene="trpB"
                     /locus_tag="PK518_07395"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRM48778.1"
                     /translation="MTASTHFAPMPDAAGFFGPYGGQLVPPHLKQAMDDINAAYAEIT
                     QRKDFQDELAQLFADYVGRPSPIFHAKRLSAQLGGAQIHLKREDLNHTGAHKINHCLG
                     EALLAKFMGKKKVIAETGAGQHGVALATACALVGIPCEIHMGQVDIEKEHPNVTKMRI
                     LGCKLVPVTRGAATLKEAVDSAFEEYLTNPTDYLYAIGSVVGPHPFPMMVRDFQSIVG
                     REAREQFQAKHGRLPDYVAACVGGGSNAMGIFTAFLNDADVKLVGVEPSGEGTDKPGR
                     HAATLSMGKPGEIHGMKCYVLESEPGVPAAVHSIASGLDYPGVGPQHSYLKDLGRVQY
                     EAVTDKECLDAFMQLSRVEGIIPALESAHAVAWAMRVAPSLPQDCNILINLSGRGDKD
                     ADYVAKLLGI"
     gene            1776..2114
                     /locus_tag="PK518_07400"
     CDS             1776..2114
                     /locus_tag="PK518_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HRM48779.1"
                     /translation="MALPPNKQRSRRQRKKMRIGEFQEFGFEYELKVKAELSPEQEEA
                     LMDRFVRELLVPRNLAAAGWVREGFVTAFTRGSASDEDRQATQAWLAAQPEVGEATVS
                     GLKDAWYVED"
     gene            2143..2895
                     /gene="aat"
                     /locus_tag="PK518_07405"
     CDS             2143..2895
                     /gene="aat"
                     /locus_tag="PK518_07405"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRM48780.1"
                     /translation="MTVPLPWLGPDDPFPLPSSGCGPDSTAPGLLAAGATLDVPRLLQ
                     AYAQGIFPWFSAGQPILWWSPDPRMVLPVAEFRLHRSLRRTLQRFRAAPGCEVRVDSA
                     FINVIQACARTPRDGQSGTWIVPAIVQAYSALHAAGHAHSVETWVNGRLAGGLYCVAL
                     GRAVFGESMFAHATDASKIALAALVCLCRRSGVALIDCQQNTGHLASLGAREIERAQF
                     LRHVEQARVLPACNWRFESVYWDELLPSSPAA"
     gene            2892..3635
                     /locus_tag="PK518_07410"
     CDS             2892..3635
                     /locus_tag="PK518_07410"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRM48781.1"
                     /translation="MTQLNDLPLQSLQFYATAPYPCSYLPGRQARSQVATPSHLIQNA
                     VYSDLVARGFRRSGMFTYRPFCDGCQACTPLRVLVNAFRPDRSQRRAWKRHAGLQARV
                     LRLCYIPEHYQLYLRYQTARHTGGGMDHDSIDQYTQFLLQSRVNSRLVEFREPGLDGQ
                     PGALKMVSILDVLEDGISAVYTFYDADPGASYGTYNVLWQIQQAHALGLPHVYLGYWI
                     AQSQKMNYKARFVPHELLLNGQWQAPPVA"
     gene            complement(3648..4349)
                     /locus_tag="PK518_07415"
     CDS             complement(3648..4349)
                     /locus_tag="PK518_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRM48782.1"
                     /translation="MLTIRKSQDRGHADHGWLNSYHSFSFAGYHDPRHMGWGNLRVIN
                     EDRIAPGTGFGAHGHRDMEIISYVLSGELAHQDSMGNVKGIPPGDVQRMSAGSGVMHS
                     EFNHAPDQVTHFLQIWIEPNVRGIAPGYEQVTVPAADKRGALRLLASPTGGPGVVRIH
                     ADASLYAGLFDGGESATLALNASRKAYVHVVRGALDVNGTPLLAGDAALLADEPCIRL
                     EQGQDAEVLVFDLAP"
     gene            4458..5396
                     /locus_tag="PK518_07420"
     CDS             4458..5396
                     /locus_tag="PK518_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM48783.1"
                     /translation="MQNTRNVLTPDNLGLLQSIAETGSFAAAARQLGLVPSALTYRVR
                     QIEDALDVLLFDRSARQARPTDAGLELLREGKRLLEDVDAIANRVRRVATGWEPQLTL
                     SVDGVISPTTMLELVEAFYALEPPTRLKWRDGILSGTAEALTSGHADLAIGVTEGASN
                     VAGLQAAPIGELRFIFVVAPHHPLARLPEPISDSQLRAHRLIAVADSARQSQVTMGIL
                     PGQDVFTVDNMRAKIQAQLRGLGAGFLPEPMVRPYVQAGHLVARAVARPPRNVRMCYQ
                     WGGSGSSVPGRALQWWLGQLQSQATRQALLENHHHF"
     gene            5480..6574
                     /locus_tag="PK518_07425"
     CDS             5480..6574
                     /locus_tag="PK518_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM48784.1"
                     /translation="MTTARPRSSKKLKPSAPHGPARRFAVIGAGMAGVACARTLAQAG
                     HTVTVFEKAPHVGGRTMALASPFGSFDTGAQYFTVRDERFQRALDTVPGLCRPWSATT
                     VRVLDAAGRVASAAPPPGEAHWVATPTMDALVQAWAEPLMREGRLHTHTAVTRIERDP
                     AHPHMWQLHCETLGGDTHLHTGFDAVLLTLPAPLSQALLHDSGLSAAWNQNLQGVDIA
                     PCWTLMLAFAHAVRPGLTTLGPQWNAARSTHHRIAWLARESSKPGRKQIERWTVQASP
                     AWSQEHLNDDGARVQAKLVKAFAEVTGIRATPTHAELRRWRFAQTQKPLGQSHLWDAA
                     TGLGAGGDWCLGHRLEDAFISGLELALAAL"
     gene            6571..7686
                     /gene="gluQRS"
                     /locus_tag="PK518_07430"
     CDS             6571..7686
                     /gene="gluQRS"
                     /locus_tag="PK518_07430"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRM48785.1"
                     /translation="MSVAARYVGRFAPSPTGPLHAGSLVAALASWLDARAAGGRWLVR
                     IEDVDLPRCQSGAAERILAQLAACGLLPDEAPWFQSGRNARYQQVLDELLARQLAYPC
                     ACTRRDIAAALLAQGLAHERHVERPYPGTCRQGLHGRAPRAWRFHAARYQGRLAGGAH
                     AEQAPHPSLSPKGRGQPVAPVLLPLPLGEGGGEGPPPGVRHAENESNMPLALYGQAKT
                     ASLSIANNGIHWTDRRLGPQFQDVARQVGDFVLRRADGLWAYQLAVVVDDADQGITHV
                     VRGEDLADNTPRQILLQSALGLVTPRYLHTPLVRAGDGEKLSKQHGAPAIDDSRPLQA
                     LGDAAEVLGLPAAGTASVAEALALWVNAWARNYNPPL"
     gene            7656..8405
                     /gene="trmB"
                     /locus_tag="PK518_07435"
     CDS             7656..8405
                     /gene="trmB"
                     /locus_tag="PK518_07435"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRM48786.1"
                     /translation="MGAKLQSAAVTETPHPTPPQSGAPAEVAHPKVIKSYVRRAGRTT
                     TGQAKALEELGPRYVLDYRAALFDAPAAFGRNAPLILEIGFGMGDATAHIAGLRAGDN
                     FLCCEVHEPGVGALLKRIGEQGLENIRILQHDAVEVLDHMLAPASLDGVHIFFPDPWH
                     KKRHNKRRLIQPPLVAKLAARLKPGGYIHCATDWQPYAEQMLQVLANEPLLANTAEGY
                     APQPDYRPLTKFENRGLRLGHGVWDLVFKRR"
     gene            complement(8468..11092)
                     /gene="alaS"
                     /locus_tag="PK518_07440"
     CDS             complement(8468..11092)
                     /gene="alaS"
                     /locus_tag="PK518_07440"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRM48787.1"
                     /translation="MSTPTFSVADIRKTFLDFFAAKGHTVVTSSPLVPGNDPTLMFTN
                     SGMVQFKDVFLGTDKRSYVRAASVQTCLRAGGKHNDLENVGYTARHHTFFEMLGNWSF
                     GDYFKRESIHWGWELLTQVYKLPADKLLATVYAEDDEAYDIWTKEIGLPPERVIRIGD
                     NKGGRYKSDNFWMMADTGPCGPCSEIFYDHGEHIPGGPPGSPDEDGDRFIEIWNHVFM
                     QFDMKEDGSVVPLPAPCVDTGMGLERLAAILQHVHSNYEIDLFDALIKAAARETHTAD
                     LANPSLKVIADHIRATAFLVADGVIPSNEGRGYVQRRIVRRAIRHGYKLGQKTPFFHK
                     LVADLAQQMGDAYPKIREQQQRITEVLKVEEERFFETLANGMEILDGALAGGAKTLPG
                     DVAFKLHDTYGFPLDLSADVCRERGLSVDEAGFNAAMEHQKNTARAAGKFKMDKALEY
                     GGAANQFTGYEHLSGTAKIVALYVDGISVAALKAGQNGVVVLDSTPFYAESGGQVGDQ
                     GVITAGSARFAVQDTQKIKADVYGHHGVLSEGTLNVGDTVQAQVDGAERAATMRNHSV
                     THLMHKALREVLGDHVQQKGSLVNAERTRFDFTHNTPVTAAQITEIERLVNAEVLANS
                     ATQARVMDIESAQQTGAMMLFGEKYGESVRVLDIGSSRELCGGTHVARTGDIGLFKVV
                     AEGGVAAGVRRVEAVTGANALAYLQSLEATVDQAAAVLRAPVAELNTRITGALDQIKA
                     LEKELAQLKGRLASSQGDELAGQAVDVKGIKVLAAKLDGADAKTLRETMDKLKDKLKT
                     AAIVLAAVDGDKVQLAAGVTADSTARVKAGELVNFVAQQVGGKGGGKPDMAMAGGTQP
                     AALPAALASVQAWVADRL"
     gene            11216..11289
                     /locus_tag="PK518_07445"
     tRNA            11216..11289
                     /locus_tag="PK518_07445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11248..11250,aa:Gly,seq:ccc)
     gene            11528..13381
                     /locus_tag="PK518_07450"
     CDS             11528..13381
                     /locus_tag="PK518_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM48788.1"
                     /translation="MALSDLAVRQAKATGKAYTLPDLDGLSLAVTAAGGRTWHFRYYW
                     AGKQKRMSLGTYPEVTLREARSLRDEARALLAKGTNPRVHRKQKRTAVRLADENTFEA
                     VYRKWLKHRGLSLKEGRQTTLSILPRIFDKDVLPTLGKRSVYEIKRPDLLEVIAKIEK
                     RRALSVAEKVRTWFNQLFRYALVIVPGLEQNPASDLDVVALPLPPVNHNPFLRMAELP
                     KLLQRLRSYRGRRQTQLGLRLLLLTGVRTGELRQATPDQFDLDRGLWIIPPDVVKQLQ
                     LDMRKKRQQPKDIPPYIVPLSIQAMEIVRHLLDEFKPAQRHLFRHDSDLKKRISENTL
                     NGALKRMGYQERLTGHGIRGTMSTALNEIGYPKVWVDAQLSHVDPNKVSATYNHAEYV
                     EQRRRMMQDWADRLDLFEQNQVEAASMPLTVHLEGVPAFPSEQTASAPSAPVAASPIL
                     LVTKPGDAMPLVSAAAHRLPAVPPPRSAAPLVPSDIQRERMELFDVFEAPHNLPVAAF
                     AKMAGKSRRWISYEIKAGNLLALNVGNRGQRVPDWHLDPLKHELIQSVLKLTRGADPW
                     QIYHALLQPRSMLRGRSALEGVTAGNLDKLVMAVSTAVKESEWTPPRVGVA"
     gene            complement(13429..14055)
                     /locus_tag="PK518_07455"
     CDS             complement(13429..14055)
                     /locus_tag="PK518_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM48789.1"
                     /translation="MESSDITAEIRMAVLGEWVPPQLADVLALQRAEEPETHAVLAGW
                     TDPGRGAELPDDGFDFALSAVARQWPGWVCEPLWHDTLAVAVAKRSHLLAYREVPCGE
                     VLKQPLICSQSMTDEPWRMTVQRVFENALQEREQTVETFDVAMTLVAAGYGIAIAPAA
                     RLASYLRRGIAVRPLAGAPTIVMAYLLRRNASLSDTQARFARRARLVS"
     gene            14161..14460
                     /locus_tag="PK518_07460"
     CDS             14161..14460
                     /locus_tag="PK518_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM48790.1"
                     /translation="MPKRSIQRGRPTGTTTYEAEPAIAFGAAVREERTNQGIAQETLA
                     HMAGIERSHMGKIERGEHVPTLPLILKIARALKCSSAHLMTLTEAKLAESAPSAD"
     gene            complement(14487..16322)
                     /gene="mobH"
                     /locus_tag="PK518_07465"
     CDS             complement(14487..16322)
                     /gene="mobH"
                     /locus_tag="PK518_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HRM48791.1"
                     /translation="MLSLFQRKRVPPTAGTPPTSAIETPKGLMRPESAASLLATPRRQ
                     KLLEHIWQRTSLSRRQFATLYLAPLERYAELVQQFPASENHHHAYPGGMLDHGLEIVA
                     YALKLRQSYLLPAGVTPEAQAAQAEAWTAGTAYAALLHDIGKIAVDLHVEHADGSVWH
                     PWHGPLRKPYRFRYRKEREYRLHSAATGLLYARLLDRDIFDWLSGYPDLWAALLYVLA
                     GQYEHAGTLGELVVQADQASVAQELGGDPSKALAAPKHALQRKLLDGLRYLLKEAFKL
                     NQAGPADGWLTQDALWLVSKTVSDKLRAHLLSQGIDGIPASNTAVFNVLQDHGIVQPT
                     PDGKAIWKATVTSDAGWSHAFTFLKLSPAMIWDAADRPAPFAGRVQVEEEQAEPTPQA
                     TTVADGPRAEGIEAASASTAPIASAAMDTGVAALLDLLGDTALPTAPEIAEAEPTPST
                     VPPPQMSLAPSQDRAEPSGAHFMAWLRQSIQTRKLIINDAKALVHTVAGTSYLVSPGV
                     FQRYAQEYLQVAALAKQEKLEGWQWVQKRFEKLGQHRKQPSGLNIWTCEVTGPRKSRR
                     LHGYLLVSPDALFQETPPDNPYLRLVNKAAKREDSALGGKADDQA"
     gene            complement(16525..>16971)
                     /locus_tag="PK518_07470"
     CDS             complement(16525..>16971)
                     /locus_tag="PK518_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRM48792.1"
                     /translation="QHPRDLFDVWQLYESGDISDGMVECFVIYLAGHNRPPHEVLFGN
                     DKDIAGEYERAFVGMTEVDCSLETLLDARARMRRELPQRLSTAHKQFLSGLARAEPDW
                     SLVQCQHAAQLPALRWKLANLETFRKRRPDDFAAQSAALDTGLGQS"
BASE COUNT         2754 a         5737 c         5584 g         2896 t
ORIGIN      
        1 aaggcaatgc cgtagcgggg gctgacgaga aagcccgcgc gctcgaaggc ggcgctgtcg
       61 ttggcccgca cgaacatggc cagggtgcca agcggtatcg ccagtgcgcc caggtgcagc
      121 acctgggcgc gggcagtgac ttcctccagc cggccactgt cctgaaagcg gtagaccagc
      181 gcgccgtagt ccagcgcggt caggccgatg ttgttgcgtt cttcgctgtg cggcgggccg
      241 tgggcgcgga tgatgtcttc gcgcgtggcc gtgaatggcg tgccgtccac gctctggtag
      301 ggctgcaggt gcatgggcgg attctgctgc cgtgccgcaa ggcgcggcaa gcgcggtggc
      361 gaatcgcgca aagtgccgtg caactgcgcg tatattgcgc atgccgccgt gggcaagccc
      421 ttggtctggc gtcttgtaag atacctattc aaaatgccac tcagccctta ttttataagc
      481 gcttacagct ataaattttg aattagacac acctgcattt tcaaccacct tctcctgcac
      541 tgcgatgact gcctccaccc acttcgctcc catgcctgat gccgctggtt tctttggccc
      601 ctacggcggc cagctggtgc cgccgcatct gaagcaggcc atggacgaca tcaacgccgc
      661 ctatgccgag atcacgcaac gcaaggactt ccaggacgag ctggcgcagc tgtttgccga
      721 ctacgtgggc cggcccagcc caatctttca cgccaagcgc ctgtccgcgc aactgggcgg
      781 cgcgcagatt cacctgaagc gcgaggatct gaaccacacc ggcgcgcaca agatcaacca
      841 ctgcctgggc gaggcgctct tggccaagtt catgggcaag aagaaggtga tcgccgaaac
      901 cggcgccggc cagcatggcg tggccctggc cacggcctgc gcgctggtgg gcattccgtg
      961 cgagatccac atgggccagg tggacatcga gaaggagcac cccaacgtca ccaagatgcg
     1021 catcctgggc tgcaagctgg tgccggtgac gcgcggcgcg gccacgctga aagaggcggt
     1081 ggacagcgcc tttgaggaat acctgacgaa ccccaccgac tacctgtacg ccatcggctc
     1141 ggtggtcggc ccccacccct tcccgatgat ggtgcgcgac ttccagagca ttgtcggccg
     1201 cgaggcgcgc gagcagttcc aggccaaaca tggccgcctg cccgactatg tggcggcctg
     1261 cgtgggcggc ggcagcaacg ccatgggcat attcacggcc ttcttgaatg acgccgatgt
     1321 gaagttggtc ggcgtcgagc cctccggcga gggcaccgac aagcccggcc gccacgccgc
     1381 caccctgagc atgggcaagc ccggcgaaat tcacggcatg aagtgctatg tgctggagag
     1441 cgaaccgggc gtgcccgccg ccgtgcacag cattgcctcg ggcctggact accccggcgt
     1501 cggcccgcag cacagctacc tgaaggactt gggccgcgtg cagtacgaag ccgtcaccga
     1561 caaggaatgc ctggatgcct tcatgcagct gtcgcgcgtg gagggcatca tcccggcgct
     1621 ggaaagcgcc catgccgtcg cctgggccat gcgcgtggcg ccaagcctgc cccaggactg
     1681 caacatcctg atcaacctgt cgggccgtgg cgacaaggac gcggactacg tcgccaagct
     1741 gctgggcatc tgattttttt caaggagttt tgagcatggc actgccaccg aacaagcaac
     1801 gcagccgccg ccagcgcaag aagatgcgca tcggcgagtt tcaggagttt ggcttcgagt
     1861 acgagctgaa ggtgaaggcc gagctttcgc ccgagcagga ggaggcgctg atggaccgct
     1921 tcgtgcgcga gctgctggtg ccgcgcaacc tggcagccgc cggctgggtg cgcgagggct
     1981 ttgtgaccgc cttcacgcgc ggctcggcca gcgacgaaga ccgccaggcc acccaggcct
     2041 ggctggcggc ccagcccgag gtgggcgagg ccacagtgag tggcctcaag gatgcctggt
     2101 acgtggagga ttgacccacg gctcaagacc caaggcccgc gcatgacagt accactgccc
     2161 tggcttgggc ccgacgaccc tttcccgcta ccgtccagcg gctgtggccc cgactccacc
     2221 gcccccggac tgctggccgc gggagccacg ctggatgtgc cgcgcctgct gcaggcctat
     2281 gcccagggca tcttcccctg gttcagcgcc ggccagccca ttctgtggtg gagccccgat
     2341 ccgcgcatgg tgctgccggt ggccgagttt cgcctgcacc gctcgctgcg gcgcacgctg
     2401 cagcgctttc gcgccgcgcc gggctgcgag gtgcgcgtgg acagtgcttt tatcaacgtc
     2461 atccaggcct gcgcgcgcac gccgcgtgat ggccagtcgg gcacctggat cgtgcccgcg
     2521 atagtccagg cctactcggc gctgcacgcc gccggccatg cgcacagcgt ggagacctgg
     2581 gtgaatggcc gcctggcggg cgggttgtac tgcgtggcgc tggggcgggc ggtgtttggc
     2641 gaatcgatgt tcgcgcatgc caccgatgca tcaaaaattg ccctggcggc gctggtctgc
     2701 ctgtgccggc gcagcggcgt ggcgctgatc gactgccagc agaacaccgg ccacctggca
     2761 tcgctgggcg cgcgcgagat cgagcgcgcc cagtttctgc gccatgtgga gcaagcgcgc
     2821 gttttgccgg cctgcaactg gcgctttgaa tccgtatact gggacgagct cctgccctca
     2881 agccctgcgg cgtgacccag ctcaacgatc ttccgctaca aagcctgcag ttctatgcca
     2941 cggcgcccta cccctgcagc tatctgcccg ggcggcaggc gcgctcgcag gtggccacgc
     3001 ccagccacct gatccagaac gccgtgtatt ccgacctggt ggcgcggggt tttcgccgca
     3061 gcggcatgtt cacctaccgg ccgttttgcg atggctgcca ggcctgcacg ccgctgcgtg
     3121 tgctggtgaa cgccttccgc cccgaccgca gccagcgccg cgcctggaag cgccatgccg
     3181 gcctgcaggc gcgcgtgctg cgcctgtgct acatccccga gcattaccag ctctacctgc
     3241 gctaccagac cgcgcgccac acgggcggtg gcatggatca cgacagcatc gaccagtaca
     3301 cgcagttctt gctgcaaagc cgcgtcaatt cacgcctggt ggagttccgt gagccggggt
     3361 tggacgggca gccgggcgcg ctcaagatgg tgtcgatact cgacgtgctg gaggacggca
     3421 tctccgccgt ctacaccttc tacgacgccg acccgggcgc cagctatggc acctacaacg
     3481 tgctgtggca gatacagcag gcccatgcgc tgggcctgcc gcatgtctac ctgggctact
     3541 ggatagcgca aagccagaag atgaactaca aggcgcgctt tgtgccgcat gagctactgc
     3601 tgaacgggca gtggcaggca ccgccagtgg cctgatacga aagcgcctta cggcgccagg
     3661 tcgaacacca gcacctcggc atcctggccc tgctccagcc tgatgcacgg ctcatcggcc
     3721 agcagcgcgg catcgcccgc cagcaagggg gtgccgttga catccagggc gccgcgcacc
     3781 acatgcacat aggccttgcg gctggcgttc agcgccaggg tggctgactc gccgccgtca
     3841 aacaatccgg catacagcga cgcatcggca tggatgcgca ccacgcccgg cccgcccgtg
     3901 ggcgaggcca gcaggcgcag ggcgccgcgc ttgtcggccg caggcacggt gacctgctca
     3961 tagccgggtg caatgccgcg cacattgggc tctatccata tctgcaggaa atgcgtcacc
     4021 tgatccggcg cgtggttgaa ctcgctgtgc atcacgcccg agccggcact catgcgctgc
     4081 acgtcgcccg gcggtatgcc cttcacgttg cccatgctgt cctggtgcgc cagctcgccc
     4141 gagagcacgt agctgatgat ctccatgtcg cggtggccat gcgcgccaaa gccggtgccg
     4201 ggggcaatgc ggtcttcatt gatgacgcgc agattgcccc agcccatgtg gcgcgggtcg
     4261 tggtagccgg caaaggagaa gctgtggtac gagttgagcc agccatggtc ggcgtggccg
     4321 cggtcttgcg atttgcgaat ggtcagcatg cggtagtcct tcaatgtcta ccggtcactg
     4381 taggtcggta cgctggcttg cgggggctat gggcttgaag gcatcattca agtcgtttga
     4441 acagattgcc tccaatcatg caaaacaccc gcaatgtgct gacccccgac aacctcggcc
     4501 tgctgcaaag cattgccgag accggcagct ttgccgccgc cgcgcgccag ctgggcctgg
     4561 tgcccagcgc gctgacctac cgcgtgcgcc agatcgagga cgcgcttgat gtgctgctgt
     4621 tcgaccgcag cgcgcgccag gcccggccca ccgacgccgg cctagagctg ctgcgcgagg
     4681 gcaagcgcct gctggaggat gtggacgcca tcgccaaccg cgtgcgccgc gtggccaccg
     4741 gctgggagcc gcagctgacg ctgtcggtgg acggcgtgat ctcgcccacc accatgctgg
     4801 agctggtgga ggccttctac gccctggagc cgcccacgcg cctgaagtgg cgcgacggca
     4861 tcctgagcgg cacggccgag gccttgacct cgggccacgc cgacctggcc ataggcgtga
     4921 ccgaaggcgc cagcaacgtc gcgggcctgc aggccgcgcc gattggcgag ctgcgcttca
     4981 tcttcgtggt cgcaccgcac cacccgctgg ccaggctgcc cgagcccatc agcgacagcc
     5041 agctgcgcgc gcaccgcctg attgccgtgg ccgattcggc ccgccaaagc caggtcacca
     5101 tgggcattct gccggggcag gacgtgttca ccgtggacaa catgcgtgcc aagatccagg
     5161 cccagctgcg cggcctgggc gcgggttttc tgcccgaacc catggtgcgc ccgtatgttc
     5221 aggccggcca cctggtggcg cgcgccgtgg cccgcccgcc gcgcaacgtg cgcatgtgct
     5281 accagtgggg tggatcgggc agctcggtgc cgggccgggc gctgcaatgg tggctggggc
     5341 agctgcaaag ccaggccacg cgccaggcgc tgctggaaaa tcaccaccat ttctgatccg
     5401 cggcgggtgc ggcgcgtgta gagtcattgc tgtctttgca ttgccctccc attgcacgaa
     5461 agttgcacga aagcccctca tgacaactgc ccgcccccgt tccagcaaaa agttgaaacc
     5521 cagcgccccg catggacccg cgcggcgctt tgcggtgatt ggcgccggta tggccggcgt
     5581 ggcctgcgcg cgcacgctgg cccaggcggg ccacaccgtc acggtatttg agaaggcacc
     5641 gcatgtgggc gggcgcacca tggcgctggc atctcccttt ggcagttttg ataccggcgc
     5701 gcagtacttc accgtgcgcg acgagcgctt tcagcgcgcg ctggacaccg tgcccggcct
     5761 gtgccgcccc tggagcgcca ccaccgtccg cgtgctcgat gccgcgggcc gcgtcgccag
     5821 cgcggcgccg ccgcccggcg aggcgcattg ggtggccacc cccaccatgg acgcactggt
     5881 gcaagcctgg gccgagccgc tgatgcgcga gggccggctg cacacgcaca ccgccgtcac
     5941 gcgcatcgag cgcgatcccg cacaccccca catgtggcag ctgcactgcg agacgctggg
     6001 cggcgacacc cacctgcaca ccggctttga cgccgtgctg ctgaccctgc cggcgccgct
     6061 gtcgcaggcg ctgctgcacg actccggcct gagcgccgcc tggaaccaga acctgcaggg
     6121 cgtggacatc gccccctgct ggacgctgat gctggccttt gcccatgccg tgcgccccgg
     6181 tctgaccacg ctgggcccgc agtggaacgc cgcgcgcagc acacaccacc gcatcgcctg
     6241 gctggcgcgc gagtcaagca agcccggacg caagcagatc gagcgctgga cggtgcaggc
     6301 cagcccggcc tggtcacagg agcacctgaa cgacgacggc gcccgcgtgc aggccaagct
     6361 cgtcaaggcc tttgccgagg tcaccggcat acgcgccacg cctacccatg ccgagctgcg
     6421 ccgctggcgc tttgcccaga cgcaaaagcc cctgggccaa agccatctgt gggacgccgc
     6481 caccggcctg ggcgccggcg gcgactggtg cctgggccac cggctggagg acgccttcat
     6541 ctccggcctg gagctggccc tggccgctct atgagcgtgg cggcgcgcta cgtcggccgc
     6601 tttgccccct ctcccacggg cccgctgcat gcgggctcgc ttgttgcggc gctggccagc
     6661 tggctggacg cgcgcgcggc cggcgggcgc tggctggtgc gcatcgaaga cgtggattta
     6721 ccgcgctgcc agagcggcgc cgccgagcgc atcctggccc agctggccgc ctgcggcctg
     6781 ctgcccgacg aagcgccctg gttccagtcc ggccgcaacg cgcgctacca gcaggtgctg
     6841 gacgagctgc tggcacgcca gctggcctac ccctgcgcct gcacccggcg cgacatagcg
     6901 gccgctctgt tggcccaggg cctggcgcac gagcgccatg tggaacgccc ctaccccggc
     6961 acctgccgcc agggcctgca cggccgcgca ccgcgcgcct ggcgctttca tgcggcgcgg
     7021 taccaggggc ggttggctgg cggtgcgcat gccgaacaag cccctcaccc ctccctctcc
     7081 ccaaaggggc gagggcaacc ggtcgcgccg gtattactcc ctctccctct gggagagggc
     7141 gggggtgagg gcccgccacc tggcgtgagg catgcagaaa atgagtcaaa tatgccttta
     7201 gcgctttatg gacaagcgaa aacagcttct ctttcaatag cgaacaacgg catccactgg
     7261 accgacaggc ggctgggccc gcagttccag gacgtggcgc ggcaggtggg cgacttcgtg
     7321 ctgcgccgcg ctgacggcct gtgggcctac cagctggccg tggtggtgga cgatgccgac
     7381 cagggcatca cccatgtggt gcgcggtgag gacctggccg acaacacccc gcgccagatc
     7441 ctgctgcagt ccgccctggg cctggtcaca ccgcgctacc tgcacacgcc gctggtgcgc
     7501 gccggcgacg gcgaaaagct ctccaagcag catggcgccc cggccatcga cgacagccgg
     7561 ccgttgcagg cgctgggcga cgccgccgag gtgctgggcc tgccggcggc cggcactgcc
     7621 agcgtggccg aggcgctggc tttgtgggtc aacgcatggg cgcgaaatta caatccgccg
     7681 ctgtgactga aaccccacac cccaccccac cccaatccgg cgcacctgcc gaggtcgccc
     7741 accccaaggt catcaagagc tacgtgcgcc gcgccggccg caccaccacc ggccaggcca
     7801 aggcgctgga ggagctcggc ccgcgctacg tgctggacta tcgggcagcc ctgttcgacg
     7861 cccctgccgc cttcggccgc aacgcgccgc tgatcctgga gatcggcttt ggcatgggcg
     7921 atgccacggc gcatatcgcc ggcctgcgcg ctggcgacaa ctttctgtgc tgcgaggtgc
     7981 acgagcccgg cgtgggcgcc ctgctcaagc gcatcggcga acagggcctg gagaacatcc
     8041 gcatcctgca gcatgacgcc gtggaggtgc tcgaccacat gctggccccc gccagcctgg
     8101 atggcgtgca catcttcttt ccagacccct ggcacaagaa gcgccacaac aagcgccgcc
     8161 tgatccagcc gccgctggtg gccaagctgg cggcgcgcct gaagcccggc ggctacatcc
     8221 actgcgccac cgactggcag ccctatgccg agcagatgct gcaggtgcta gccaacgagc
     8281 cactgctcgc caacacggcc gagggctacg cgccccagcc cgactaccgg cctctgacca
     8341 agtttgagaa ccggggcctg cgcctgggcc atggggtgtg ggatctggtc ttcaagcgtc
     8401 gctgaatcca gataaaaaac agcgcctgcc cttaccaggc aagcgctgtc agctacacaa
     8461 tcagtagtta cagacggtct gccacccagg cctgcacgct ggccagagcc gctggcaagg
     8521 ccgccggctg cgtgccgccg gccatggcca tgtcgggctt gccgccgccc ttgccgccga
     8581 cctgttgggc cacgaagttg accagctcgc cggccttgac acgggccgtg ctgtcggccg
     8641 tcacgccggc ggccagctgc accttgtcgc catccacggc ggccagcacg atggcggcgg
     8701 tcttgagctt gtccttgagc ttgtccatgg tctcgcgcaa ggtcttggcg tcggcgccgt
     8761 ccagcttggc ggccagcacc ttgatgccct tgacatcgac ggcctggccg gccagctcgt
     8821 cgccctggct ggaggccagc ctgcccttga gctgagcgag ctctttttcc agcgccttga
     8881 tctggtccag cgcgccggtg atgcgtgtgt tcagctcggc caccggcgct ctgagcacgg
     8941 ccgccgcctg gtccacggtg gcttcgagcg actgcaggta ggccagcgcg ttcgcgcccg
     9001 tcacggcctc gacgcggcgc acgcccgcgg ccacgccgcc ctcggccacg accttgaaca
     9061 ggccgatgtc acccgtgcgc gccacatgcg tgccgccgca cagctcgcgg ctgctgccga
     9121 tgtcgagcac gcgcacgctc tcgccgtatt tctcgccgaa cagcatcatg gcgccggtct
     9181 gctgcgcgct ttcgatgtcc atcacgcgcg cctgggtggc gctgttggcc agcacctcgg
     9241 cattcaccag gcgctcgatc tcggtgatct gcgccgccgt caccggcgtg ttgtgcgtga
     9301 agtcgaagcg cgtgcgttcg gcattcacca ggctgccctt ttgctgcacg tgatcgccca
     9361 acacctcgcg cagcgccttg tgcatcaggt gcgtgacgga gtggttgcgc atggtggcgg
     9421 cacgctcggc gccatcgacc tgggcctgca ccgtgtcgcc cacgttcagc gtgccttcgc
     9481 tgagcacgcc atggtggccg tagacgtctg ccttgatctt ttgcgtgtcc tgcacggcaa
     9541 agcgcgcgct gccggcggtg atgacgccct ggtctccgac ctggccgccg ctttcggcgt
     9601 agaacggcgt gctgtccagc accaccacgc cgttttgacc ggctttcagc gctgcaacgc
     9661 ttatcccatc tacataaagc gctactatct ttgcagtacc cgagagatgc tcgtagccgg
     9721 tgaactggtt ggcagcaccg ccgtactcca gcgccttgtc catcttgaac ttgccggccg
     9781 cgcgggcggt gttcttctga tgctccatgg cggcgttgaa gccggcctcg tccacgctca
     9841 agccccgctc gcgacacacg tcggccgaca gatcgagcgg gaagccatag gtgtcgtgca
     9901 gcttgaaggc cacgtcgccg ggcagggtct tggcaccgcc ggccagcgcg ccatccagga
     9961 tctccatgcc gttggccagg gtctcgaaga agcgctcttc ttccaccttg agcacctcgg
    10021 tgatgcgctg ctgctgctcg cggatcttgg ggtaggcgtc gcccatctgc tgcgccaggt
    10081 cggccaccag cttgtggaag aacggcgtct tctggcccag cttgtagcca tggcgtatgg
    10141 cgcggcgcac gatgcgccgc tgcacgtagc cacggccctc gttgctcggg atcacgccgt
    10201 cggcgaccag gaaggcggtg gcgcggatat ggtcggcaat caccttcagc gacggattgg
    10261 ccaggtcggc ggtatgcgtc tcgcgcgcgg cggccttgat gagcgcgtcg aacaggtcga
    10321 tctcgtagtt gctgtgcaca tgctgcagga tggcggccag gcgctccagg cccatgcccg
    10381 tgtccacgca gggcgcgggc agcggcacga cggagccgtc ctccttcatg tcgaactgca
    10441 tgaacacatg gttccagatc tcgatgaagc ggtcgccatc ctcatcgggg ctgcccgggg
    10501 ggccgccggg gatatgttcg ccgtggtcgt agaagatctc cgagcagggg ccgcaggggc
    10561 cggtgtcggc catcatccag aagttgtcgc tcttgtagcg cccgcccttg ttgtcgccga
    10621 tgcggatcac gcgctcgggc ggcaggccga tctcctttgt ccagatgtcg taggcctcgt
    10681 cgtcctcggc atacacggtg gccagcagct tgtcggcggg cagcttgtag acctgggtca
    10741 ggagctccca gccccagtgg atggattcgc gcttgaagta gtcgccgaac gaccagttgc
    10801 ccagcatctc gaagaaggtg tggtggcgcg cggtgtagcc cacgttctcc aggtcgttgt
    10861 gcttgccgcc ggcgcgcagg caggtctgca ccgaggccgc gcgcacatag gagcgcttgt
    10921 cggtgcccag gaacacatcc ttgaactgca ccatgcccga gttggtgaac atcagcgtcg
    10981 ggtcgttgcc cggcaccagg gggctggacg tcaccacggt gtggcccttg gcggcgaaga
    11041 aatccaggaa ggtcttgcgg atgtcggcga cagagaaggt gggagtgctc atggtgtgtg
    11101 cttaggcgat acgacgcgcc gcaggcaaga acaagccgcg tgcgccgacc gtgtgaaaat
    11161 caaccgggca ttatagattt ggccaccacc cccgccccct ataatgcgag ccttcgcggg
    11221 tgtagttcaa tggtagaacg gcagcttccc aagcttcata cgagggttcg attcccttca
    11281 cccgctccac tgcatcaatc tgcggacttc cgcagaagtc caagagagtc tgcaagtcgt
    11341 tgccgcacaa ggacttttcc tctctttgat gctctgccgt agtccactgc gatccgccta
    11401 cagccgggac ttttaagtcc acaaacaagt ccatttttgc gggcgcggct gattccggat
    11461 tgttgatgga ggggcgaggt cgacgtgacc agcatctggt tcctgactca tgttcaggag
    11521 caagagcatg gcactctcag acctggccgt tcgacaggcc aaggcaactg gcaaggcata
    11581 caccctccct gacttggatg gcctctccct ggccgtcacg gctgcagggg gcaggacttg
    11641 gcacttccgc tactactggg cgggcaagca gaagcggatg tcgctcggca cctacccgga
    11701 ggtgacgctt cgcgaggccc gcagcctgcg cgacgaagcg cgcgccctgc tggccaaagg
    11761 caccaatcct cgcgttcacc gcaagcagaa gcgcaccgct gtccggctcg ccgacgaaaa
    11821 caccttcgag gcggtgtatc gcaagtggct caagcatcgc gggctcagcc tcaaggaagg
    11881 ccggcagacg accctctcga tacttccacg gatcttcgac aaggatgtgt tgcccacctt
    11941 gggcaagcgg tcggtctatg agatcaagcg tcccgacctc ttggaggtga tcgccaagat
    12001 cgagaagcgc agggcgctct ccgtcgccga gaaagtcagg acgtggttca accaactgtt
    12061 ccgctacgcg ctggtgatcg tgccgggcct ggagcagaac cctgcctccg atctcgatgt
    12121 ggtggcgctg ccgctgccac ccgtcaacca caacccgttc ctgcgcatgg ccgagctgcc
    12181 gaagctgttg cagcggctgc gcagctatcg cggcaggcgg cagacccagc tcgggctgcg
    12241 gctattgctg ctgaccggcg tgcgaaccgg cgagctgcga caggcgacgc cggatcaatt
    12301 cgatctggac cgtgggctgt ggatcatccc gcccgacgtc gtgaagcaac tccagttgga
    12361 tatgcgcaag aagcggcagc agccaaagga catcccgccc tacatcgtgc cgctgtcgat
    12421 tcaggccatg gagatcgtcc ggcacctgct ggatgagttc aagccggccc agcgccacct
    12481 gttccggcac gacagcgact tgaagaagcg catcagcgag aacacgctca atggtgcgct
    12541 caaacgcatg ggctatcagg agcgcctgac cggacacggt atccgcggca cgatgtccac
    12601 tgcgctcaac gagatcggct acccgaaggt ctgggtggat gcacagctct cgcatgtcga
    12661 tcccaacaag gtcagcgcga cctacaacca tgccgagtac gtggagcagc gtcgccgcat
    12721 gatgcaggac tgggccgatc ggctcgacct cttcgagcag aaccaggtcg aggcggccag
    12781 catgccgctc accgtgcatc tggaaggcgt gcccgcgttt ccgagtgagc aaaccgcaag
    12841 cgcgccctcc gcgccggttg ccgcttcgcc aatcctgctc gtgacgaagc cgggcgacgc
    12901 catgccgttg gtttctgccg ccgcacatcg gctgccggcg gttccgcccc ctcgatcggc
    12961 cgcgccgctg gtgccttcgg acattcagcg cgagaggatg gaactgttcg atgtcttcga
    13021 agcgccgcac aaccttcccg ttgcggcgtt tgccaagatg gcgggcaagt cccgcaggtg
    13081 gatcagctac gagatcaagg cgggcaactt gctggcgttg aacgtgggca accgcggcca
    13141 gcgcgtgccg gactggcacc tcgacccact caagcacgag ctgatccagt ccgtcctgaa
    13201 gctgaccagg ggtgcggacc cttggcagat ctaccatgca ctgctgcaac cgcgctcgat
    13261 gctgcggggg cgttcggcac tggagggcgt gacggccggc aatctcgaca agctcgtcat
    13321 ggcagtgagc acagccgtga aggaaagcga atggaccccg ccgcgagtcg gggtcgccta
    13381 gcagcaggaa tgcgggatcg tggcgaaacc gcgatccccg cacgtctgtc aggacaccaa
    13441 gcgcgcgcgg cgggcgaatc ttgcctgggt gtccgacaag gaagcgttgc ggcggagcag
    13501 gtaagccatc acgatcgtcg gtgcgccggc cagcggccgc acggcgatgc ctcggcgtag
    13561 gtagctcgcc agtctcgcgg caggcgcaat ggcgatcccg tacccggcgg caaccagcgt
    13621 catcgccaca tcgaatgtct ccaccgtttg ctctcgctct tgcagcgcgt tttcgaacac
    13681 acgctgcacg gtcatgcgcc atggttcatc ggtcatggac tgcgagcaga tcaagggctg
    13741 cttgagcact tccccgcacg gcacttcacg gtaggccagc aggtgggagc gcttggccac
    13801 ggcgaccgcc agcgtgtcat gccacagcgg ttcgcatacc cagccaggcc actgccgggc
    13861 aaccgcagac aaggcgaagt cgaagccgtc gtccggcagc tccgcgcctc gaccgggatc
    13921 tgtccagccg gccaggacgg catgagtctc cggctcttcg gcacgctgca gggcgagcac
    13981 gtcggcgagc tggggaggca cccattcgcc gaggacggcc atgcgaattt cggcggtgat
    14041 gtcactcgat tccacgtcca gctcccccag gcggtgaact cgtggcaact gatctcgaag
    14101 ccgttggatg agttaagttt aacgtggttt aaatcgtgac cgtgttcgac gctcttggca
    14161 atgccaaagc gttcgatcca aaggggccgc cccaccggca ccactaccta cgaagcggaa
    14221 ccagccatcg cgtttggggc tgccgtgcgg gaagaaagaa ccaaccaggg catcgctcag
    14281 gaaacgctgg cccacatggc cggcatcgaa cggtcgcaca tgggcaagat cgagcgcggc
    14341 gagcatgtcc ccacgctccc tctcatcctc aagattgccc gtgcgctgaa atgcagttcc
    14401 gcccacttga tgactctgac cgaagccaag ctggcagagt cggccccatc cgcagattaa
    14461 agccggcccg cacagcggtc gcatgcctac gcctgatcgt cggccttgcc cccaagggct
    14521 gaatcttcgc gctttgcggc cttgttgacg agccgcaggt atgggttgtc tggcggcgtc
    14581 tcctggaaca gcgcatccgg gctgacgagc aggtagccgt gcagccggcg cgacttgcgc
    14641 ggccccgtga cttcgcaggt ccagatgttc agcccgctcg gctgcttgcg gtgctgtccc
    14701 agcttctcga agcgtttctg tacccactgc cacccctcca gcttctcctg cttggccagc
    14761 gcggcgactt gaaggtactc ctgcgcgtag cgctggaaca cgccggggct gacgaggtag
    14821 ctcgtaccgg cgacggtatg caccagggcc ttggcgtcgt tgatgatgag cttgcgcgtc
    14881 tggatgctct gacgcagcca ggccatgaag tgcgccccgg agggctccgc gcgatcctgg
    14941 gaaggcgcga ggctcatctg cggcgggggc acggtcgaag gggtcggctc ggcctcggcg
    15001 atctccggcg ctgtgggtag agcggtgtcg cccagcaggt cgagcagcgc ggccacgccg
    15061 gtgtccatgg ctgcggatgc gatcggcgcc gtgctcgcgg atgcagcttc gatgccttcg
    15121 gcgcgcggcc catcggccac cgttgtcgcc tgcggcgtcg gctccgcctg ttcctcttcg
    15181 acctgcacac ggcctgcgaa cggcgccggc cggtcggcgg catcccagat catcgccggc
    15241 gagagtttca ggaaggtgaa ggcgtgcgac cagccggcgt cgctggtgac agtagccttc
    15301 cagatcgcct tgccatccgg cgttggctgc acgatgccgt gatcctgcag cacgttgaac
    15361 accgccgtat tgctcgccgg aatgccgtcg attccttgcg atagcagatg tgcgcgcagc
    15421 ttgtcggaga cggtcttgct caccagccac aaggcgtctt gggtcagcca accgtccgcc
    15481 ggaccggcct ggttcaactt gaatgcttcc ttgagcaggt agcgcaagcc gtcgagcaat
    15541 ttgcgttgca gcgcatgctt gggcgccgcc agcgccttgc tgggatcgcc gccgagttcc
    15601 tgggcgaccg atgcctggtc ggcctgcacg accagctcgc cgagcgtgcc ggcgtgctcg
    15661 tactggccgg ccagcacgta cagcagcgcg gcccagaggt cgggatagcc gctgagccag
    15721 tcgaagatgt cccgatcgag aaggcgcgcg tagagcagcc cggtggcggc gctgtgcagg
    15781 cggtactcgc gctcctttcg gtaacggaag cggtagggct ttcgcagcgg gccgtgccag
    15841 ggatgccaga cgctgccgtc ggcatgctcg acgtgcagat cgaccgcaat cttgccgatg
    15901 tcgtgcagca gggccgcgta ggccgtgcct gcggtccagg cttcggcctg ggccgcctgt
    15961 gcctccggcg tgacgcccgc aggcagcagg tatgactgcc gcagcttcag cgcataggcg
    16021 acgatctcca ggccgtggtc cagcatgccg cccggatagg cgtggtggtg gttctcggag
    16081 gccgggaact gctggaccag ctcggcgtag cgctccagtg gggcgaggta gagcgtggcg
    16141 aactgccggc gcgagagcga tgtgcgctgc cagatgtgtt ccagcagttt ctgccggcgc
    16201 ggcgtggcca gcagcgatgc ggccgactcc ggccgcatca accctttcgg agtttcgatg
    16261 gcggatgtgg gcggtgtgcc ggcggtgggt ggcacccgtt tgcgctggaa cagcgagagc
    16321 atggcgaacc ccggatgacg ggctgctcgg ggcgcctttt ggccttttca ggatagggcc
    16381 tttccccttg gcgcccttcc tttgcccctt cccagccctt tggcctttgt cggaagcctt
    16441 tgggtatagg gccgctgctg cgcgggcgcc acgatgaatg cggcatgggg caatcccggc
    16501 aagtggggcg ctgcgggatg ttcgtcagct ctggcccaag ccggtgtcga gggcggcgga
    16561 ttgcgctgcg aagtcgtcgg gacggcgctt gcggaaggtt tcgagattgg cgagcttcca
    16621 gcgcagtgcc ggcagttgcg cggcgtgctg gcactgcacc agcgaccagt ccggttctgc
    16681 gcgcgccagc ccgctcagaa actgcttgtg cgcggtgctc agtcgctgtg gcagctcgcg
    16741 ccgcatcctg gcgcgagcgt cgagcagtgt ctccagggag cagtccactt cggtcatgcc
    16801 gacaaaggcc cgctcgtact cgccggcgat gtccttgtcg ttgccgaaca gcacttcgtg
    16861 gggcggccgg ttgtggcccg ccagatagat cacgaagcac tcgaccatgc cgtcgctgat
    16921 gtcgcccgat tcgtagagct gccacacgtc gaacaggtcg cgggggtgct g
//
LOCUS       DAOMTQ010000057        16966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_488_length_16966_cov_25.037017, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000057 DAOMTQ010000000
VERSION     DAOMTQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 16966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16966
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_488_length_16966_cov_25.037017"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK518_07475"
     CDS             <1..358
                     /locus_tag="PK518_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRM48793.1"
                     /translation="YTPSGKSIQAKGIVPDVMLDETAEGDIYASLRMREADLEKHLTS
                     GQGEEVKDAAREKAREEARKRLEEEAKKPVAERRLPEFGSDKDFQLTQALNRLKGHTV
                     VASKTLTERKEEKKDE"
     gene            419..1177
                     /gene="moeB"
                     /locus_tag="PK518_07480"
     CDS             419..1177
                     /gene="moeB"
                     /locus_tag="PK518_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRM48794.1"
                     /translation="MNDDQLLRYSRHILLDEIGIEGQERILAAHVLIVGAGGLGSPAA
                     LFLGSAGVGRLTLVDDDVVDLTNLQRQIAHTTDRVGSPKVDSIAQAVHAINPLVAIDT
                     VCQRVDAAALDALVAHADVVLDCSDNYRTRQAINAACVRQRRPLVAGAAIRFDGQISV
                     YDPRGADQPCYACLFPPDAQFEEVACSTMGVFAPMVGIIGSMQAAEALKLITGVGSSL
                     AGRLLMLDGRDMQWTEMRTQRDPDCPVCATPAHR"
     gene            1284..1703
                     /locus_tag="PK518_07485"
     CDS             1284..1703
                     /locus_tag="PK518_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM48795.1"
                     /translation="MKLRTYIVEDNATIRENLIGTLEELAEVQAIGCAETEDEGKAWL
                     TAHAAQWDLAIVDLFLRQGSGLGVLAACRDRASEQKMVVLSNYATPDVRMRCAQLGVD
                     AVFDKSNEIDALVDYCIVHGTQRAAAAGAPVNRSACS"
     gene            complement(1681..2313)
                     /locus_tag="PK518_07490"
     CDS             complement(1681..2313)
                     /locus_tag="PK518_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM48796.1"
                     /translation="MIKIGIVDDHAIVRSGLRQFFSEHVDLRVVGEAANGREAIDLVR
                     AQEIDVLVMDLSMPGQSGLDALAMLRAKAPDMGILILSGYPEEHYAINLIRQGASGYL
                     NKECDPKEIVEAIRAIALGRRYLTPAVAELLAQQLNRKGDAPPHEQLSEREFQVFLKL
                     ARGETAGDIAKSLSLSVKTVSTYRTRLMEKMGLSSNSDLTYYALKNRLID"
     gene            2423..3766
                     /locus_tag="PK518_07495"
     CDS             2423..3766
                     /locus_tag="PK518_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRM48797.1"
                     /translation="MIWSRLRKMAISLPAALLAVVLLVGINETGYMRSHDAVGDLARS
                     HATRTALDRLLQNMLNAETGLRGYLLTGDDRYLQPYNEAVATINSNLDELRQTYQRLP
                     QDQADFAQLSQQIARKLSELDLTLNLRRQNNDDAWKFVLSTDVGKDNMDAIRAIAARL
                     VERSAQQAQGSTQEILHSLMLSRIGIATVAVIGLLAFYMYLRQTNALQEANEREQEVL
                     ARERDRLEQLVRERTASLSALANHLQQVREDERAHLARELHDELGALLTAAKLDVARL
                     KSRIDMQVPEHAERIQHLTETLNNGIALKRRIIENLRPSSLSNLGLTASLEILTRDFA
                     QSSNVQVEISLEEAHLSDTAQLTVYRLVQESLTNIGKYANARKVLVCVHNHTNHVSVQ
                     VRDDGAGFDTTRIRADAHGLLGMRHRVEAAGGSLTVSSTPGEGTLVSAVLPHRIA"
     gene            3909..4073
                     /locus_tag="PK518_07500"
     CDS             3909..4073
                     /locus_tag="PK518_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRM48798.1"
                     /translation="MLHYAVVFLVIALVAAVFGFGGIAAGAVGIAKVLFFVFVIMAVV
                     SFIIGLLKKN"
     gene            4139..4531
                     /locus_tag="PK518_07505"
     CDS             4139..4531
                     /locus_tag="PK518_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48799.1"
                     /translation="MTVQTTTKKALETAHQAADDILDGASDTVQSARDMANHSLDKAD
                     SRIHHLRQDMDPRIDDIAAKAQDLASRGIAYCANTSDRARRQLRQAADTTTRYVSEKP
                     GTSLLIAAAAGAAFATAVMLARRSGERH"
     gene            4670..4984
                     /locus_tag="PK518_07510"
     CDS             4670..4984
                     /locus_tag="PK518_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRM48800.1"
                     /translation="MKYASAIAFALLTGATIVSTGCSVARDQQTVGSYVDDAAITTAV
                     KAKMAEDKGVSAAAISVETLNGTVQLSGFAKSQAEKDRAESIARNTKHVRDVRNSIVV
                     RP"
     gene            complement(5181..5669)
                     /gene="coaD"
                     /locus_tag="PK518_07515"
     CDS             complement(5181..5669)
                     /gene="coaD"
                     /locus_tag="PK518_07515"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRM48801.1"
                     /translation="MAQITAVYPGTFDPITLGHEDLLRRAARMFDRVIVAVAIAHHKK
                     TMFSLDERMDMAREALADCPQVQVEPFEGLVTEFTAARGGTAMVRGLRSGTDFDYEFQ
                     LAGMNRALVPQIETVFLSPASQYQFISSTLVREIAQLGGDVAQFVSPAVLRRLQARVA
                     KA"
     gene            complement(5817..6434)
                     /gene="rsmD"
                     /locus_tag="PK518_07520"
     CDS             complement(5817..6434)
                     /gene="rsmD"
                     /locus_tag="PK518_07520"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRM48802.1"
                     /translation="MSGPARRAKPGPKPAPKPAAARGAGEVRIIGGQWKRTRLPVAER
                     PGLRPTPDRVRETLFNWLGQDLTGWRCLDAFAGTGALGFECASRGAASVLLVEGDAGL
                     VRQLELLRQRLQATAVRVQRDDGVAALRQCAPASMQLLLIDPPFDGALFAPALQAAAQ
                     AVAPDGFIYLEAPSAWTDAELNPLGLVLHRHLKAGAVHAHLLRRQ"
     gene            complement(6431..7777)
                     /locus_tag="PK518_07525"
     CDS             complement(6431..7777)
                     /locus_tag="PK518_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRM48803.1"
                     /translation="MKKIAARALLVSAGALFCLQNAWALLPIQHWTDASGARVWLVHS
                     PAIPMVDVQIDFDAGSRRDPAPQAGLAAAAALMSAKGVAAGAANEAAMDENDLGEAWA
                     DLGASFGAQAGRDSFQYALRSLTEPDLLERAAQLAARQIAQPSFPPAVWASERARWSA
                     AIKESYTRPGPVAAKAFGSAIYGDHPYGQKPSEETLARIEVPDLQAFHERNVAACRAR
                     VSIVGALDRVQAQALVQRLLARLPARGAADCAPLPAVPDAQPLQKAVREDIPFAAAQA
                     QVLIGQLGIARRDPDFLALLVGNHILGGGGFTSRLTQEVREKRGLSYSVYSDFSPGLN
                     RGGFVIGLQTRPDQAAEAVQVAHDTLARYVADGPTEAELRAAKDNLIGGFALRIDSNR
                     KLLANVANIAWNDLPLDYLEHWTARVNALSLAEVRAAMQRHLQPARMATVIVGGQP"
     gene            complement(7805..9256)
                     /locus_tag="PK518_07530"
     CDS             complement(7805..9256)
                     /locus_tag="PK518_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRM48804.1"
                     /translation="MKRAIPLLGLLACLSAAAQTPAQNVPPAAAPPALLAQASTASGA
                     QLFTLKNGMQLIVQPDRRAPTAMHMVWLRVGSMDEVDGRSGVAHVLEHMMFKGTKKLA
                     PGEFSRRVAALGGQENAFTSRDYTGYYQQIPANRLADVMRLESDRFAHNQWPDAEFTK
                     ELAVVKEERRMRTEDQPRAALIEQLYAATFIASPYRRPVVGWMSDLDSLTANDARQFY
                     RQWYAPGNAAVVVAGDVDPQQVLALAQKTYGAIPARALPARKPRTEPEQKGLRRIDFK
                     APAEQAYVALAFHVPGVERIEDMQDGDRDGLALMVLSAVLSGYDGARLERALTQGAER
                     VADSADSHASVFGRGPALFMLTGVPAAGKTAAQVETALRAQLERVAREGVSEAELSRV
                     KTQWAASTIYARDSLYSQASDLGSNWVQGLPLDADERLLRLLRAVTPEQVQAVAARYF
                     GDDQLTVATLLPQPLAAGAKRAAPKPADGTRMH"
     assembly_gap    9282..9381
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9442..10584
                     /gene="ftsY"
                     /locus_tag="PK518_07535"
     CDS             9442..10584
                     /gene="ftsY"
                     /locus_tag="PK518_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRM48805.1"
                     /translation="MFSFFKKKSTAPAEAPIETPAAPPPDTETSASGGAMGWLRKSFG
                     AAPADREAPADQEAPALPATTDSTPPTVALPTPKPEGERQGWMERLKSGLRKTGSSIA
                     TVFTGTQINDALYEELEDALLMADTGVKATQHLLDDLKRRVKDSKTTEPAAVKALLAD
                     ALADLLKPLEKPLVIGEHQPTVIMVAGVNGAGKTTSIGKLTKHLADHGETVLLAAADT
                     FRAAAREQLGVWATRNTVEIISQEGGDPAAVSFDAVTAGRARGKDVVLVDTAGRLPTQ
                     LHLMEELKKIRRVVTKAEASAPHEVLLVIDGNTGQNALTQVRTFDDALQLTGLIVTKL
                     DGTAKGGVLAAIAQERPVPVYFIGVGEKVEDLETFNAREFAQALLA"
     gene            complement(10586..10912)
                     /gene="cutA"
                     /locus_tag="PK518_07540"
     CDS             complement(10586..10912)
                     /gene="cutA"
                     /locus_tag="PK518_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRM48806.1"
                     /translation="MPGAAHPLAVVTTTVASAADAQRLAAGAVRSRLAACVQVEAIAS
                     HYVWQGAQQEDAEWRLVCKTLPAAAGALCDWLRAQHPYQVPQLLTHTVEAEADYAGWV
                     AQQVDG"
     gene            complement(10925..12034)
                     /locus_tag="PK518_07545"
     CDS             complement(10925..12034)
                     /locus_tag="PK518_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48807.1"
                     /translation="MLSDMSGQYSDSGGRGSVEAARMAVEDFGAKVLGKPVEVVFADH
                     QNKADIGGAKARQWWDAEGVDMIIDLNNSAIASAVNVLAKERNKIAIATGALSNALTN
                     EFCAPTAIHYTVDSYSIASAAVDAVLKQGKKDWFIIAVDYSYGKVTSEIITNFVKEGG
                     GRVLGTVYHPFNAGDFSSFLMQAQASKAQVVALANATNDSINTIKGAREFGMNKTTTI
                     VPQLMYINDVHGLGLEQGQGLVFATAYYWDRNDESRAWAQKYQARMKKMPSMNQASIY
                     SAVTTYLKAVEQAQTDDGLAVAGQMKKMKINDVFSQNGYVREDGRMVHDMFLVQVKSP
                     KESKGPWDYYKVLDTIAGEKAFQPLAKSSCYLVKK"
     gene            complement(12179..13243)
                     /locus_tag="PK518_07550"
     CDS             complement(12179..13243)
                     /locus_tag="PK518_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HRM48808.1"
                     /translation="MISYDVIECGQPLVKSERATPRPVGDQVLVRIEAAGVCHSDLHI
                     WHGSYDLGHGKKLSMADRGLKLPLTMGHEIAGEVIAVGDAVQGVTVGKRYVVFPWHGC
                     GECSVCKRGDENLCLAGKSMGVFQPGGYADHVLVSHARYLVDIGDMPPVQAAPYACSG
                     LTTYSALKKIPPQVLRDEKLVLIGAGGLGLMAIGLAKAMGSTGVIVIEPDETKHAAAM
                     QAGALAVFNPRQDGYLAQVRQAAGGAVWAVIDCVGSSQTVQAGIDMLTKGGQLVQIGL
                     FGGHIDLPTPSMALRAITYQGTYVGNLRELQELMQLVKDKQLQSVPTTCMHFSKAFTA
                     LLALEEGKAVGRQILTPEGA"
     gene            13453..14388
                     /gene="rpoH"
                     /locus_tag="PK518_07555"
     CDS             13453..14388
                     /gene="rpoH"
                     /locus_tag="PK518_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRM48809.1"
                     /translation="MALQSASAVTTLAPANPWALVPPLGNLDAYISAVNRLPLLTLEE
                     EQRFARQLKDDNDVEAAGRLVMSHLRLVVSISRQYLGYGLPHGDLIQEGNVGLMKAVK
                     RFDPDQGVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKHGFKAD
                     AIDADTHRETLSEHEIDLVAEQLNVKREEVIKMETRMAGGDVLLDPAPSDDGEQAFGP
                     IAYLADAAHEPTAMIESRQRDQLATDGIATALASLDARSRRIVEQRWLQVNDDGSGGM
                     TLHELAAEYGVSAERIRQIEVAAMKKMKQALVEYA"
     gene            14468..15475
                     /locus_tag="PK518_07560"
     CDS             14468..15475
                     /locus_tag="PK518_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRM48810.1"
                     /translation="MFRYYSTRRNLLALALATVCGSAGAADKGAAPEMPSWPTKPLRI
                     VVGFPPGSSPDLTARSFAEPLAQALGQPLIVENKVGAGGNIAADAVARARDGHTIGLF
                     INGNLTIARLLNPRLPYDPDKDLAPLSLVGVSPLVLAAPVGLGDATSDARAFLQAARQ
                     AGNRWSYGTPGVGTVGHLGTELLKARTGMAPVHIPYPGYPQVATAMLGGQLQMALLPP
                     ALAQAQERAGKLRLLGVTSAGRSALVPHVPSLAEAGVRDFQLEIWNAFAAPAAMPAPV
                     RARLSALISEIARSPEVRAKLFQQGWQAVGSSSEGLANRMRADTAAMGRVIREQAIRA
                     E"
     gene            complement(15483..16094)
                     /locus_tag="PK518_07565"
     CDS             complement(15483..16094)
                     /locus_tag="PK518_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRM48811.1"
                     /translation="MLKRNTLKKIAIGALWVGAAGIFSACSPKGPKFQGVDLTGAEYG
                     RDLPLTDQFGKERSIKDFAGKVVVVFFGYTQCPDVCPTSMSELAEVKRSLGADGDKLQ
                     GIFVTVDPERDTPEMLKGYMASFDPSFIALRGTPEQLVAVAKDFKIYFKRVDGQTPTS
                     YTMDHSAGSYVYDTKGRLRVYHRYGAGAQSLAADVRALLDEGR"
     gene            complement(16104..16964)
                     /gene="cyoE"
                     /locus_tag="PK518_07570"
     CDS             complement(16104..16964)
                     /gene="cyoE"
                     /locus_tag="PK518_07570"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRM48812.1"
                     /translation="MAQFYALTKPRVVQLIVFCAFIGMVLAVPSLPSRAQWGLMAIAS
                     AGIWLVAGAAAAFNCLVERHIDARMKRTAWRPTARGELSNRQALAFSALLCTAGSALL
                     YWWVNALTMWLTFATFVGYAVVYTVILKPRTPQNIVIGGASGAMPPVLGWAAMTGDVG
                     AEALILFLIIFLWTPPHFWALALYRVEDYRKSGLPMLPVTHGSEYTRLQVFLYTLILF
                     AGCLMPFVYGMSSWIYLAAAVLLGTGFCLYGYWLWRDYSDALARKTFRFSLVHLSLLF
                     AALLVDHYVF"
BASE COUNT         2898 a         5807 c         5553 g         2608 t
ORIGIN      
        1 ctacacgccc agcggcaagt ccatccaggc caagggcatc gtgcccgacg tgatgctcga
       61 cgagacggcc gagggtgata tctacgcatc gctgcgcatg cgcgaggccg acctggaaaa
      121 gcacctgacc agcggccagg gcgaggaggt caaggacgcc gcacgcgaaa aggcccgcga
      181 ggaggcccgc aagcgcctgg aggaagaggc caagaagccg gtcgccgagc gccgactgcc
      241 cgagttcggc tcggacaagg acttccagct cactcaggcg ctgaaccgcc tcaagggcca
      301 caccgtggtg gccagcaaga cgctgaccga gcgcaaggaa gaaaaaaagg acgaataagg
      361 cccctctgcc ctaccccaaa ggccgggcca gcccggcctt tttcacgccc gccacgccat
      421 gaacgacgac caactcctgc gctactcgcg ccatatcctg ctcgacgaga tcggcatcga
      481 ggggcaggaa cgcatcctgg ccgcgcatgt gctgatcgtg ggcgccggcg gtctgggctc
      541 accggcggcg ctgttcctgg gctcggccgg tgtggggcgc ctcacgctgg tggacgatga
      601 cgtggtcgat ctgaccaacc tgcagcgcca gatcgcccac accacggatc gcgtgggcag
      661 ccccaaggtg gactccatcg cccaggcggt gcacgccatc aacccgctgg tggcgataga
      721 cacggtatgc cagcgcgtag acgccgcagc gctggatgcg cttgtggcgc atgccgacgt
      781 ggtgctcgat tgcagcgaca actaccgcac gcgccaggcc atcaatgccg cctgcgtgcg
      841 ccagcgccgg ccgctggtgg cgggcgctgc catccgcttt gacggccaga tctcggtcta
      901 cgacccgcgc ggcgcggacc agccctgcta cgcctgcctg ttcccgcccg acgcccaatt
      961 cgaagaggtg gcctgctcca ccatgggcgt gttcgccccc atggtcggca tcattggcag
     1021 catgcaggcg gccgaggccc tgaagctcat cactggcgtc ggcagctcgc tggccgggcg
     1081 tctgctgatg ctggacgggc gcgacatgca atggaccgag atgcgcaccc agcgcgaccc
     1141 ggattgcccc gtctgcgcga caccagcgca caggtaggaa cgcacctacg gccactgccc
     1201 gcaactgccg gcaagcacgc agcccgagtg cttctacagt agcgccctgg cctattcgct
     1261 cctgcgccag gcaggaaaac cccgtgaaac tgcgcaccta catcgtcgaa gacaacgcca
     1321 ccatccgcga gaacctgatt ggcacgctgg aggagctggc cgaggtgcag gccataggct
     1381 gcgccgagac cgaggatgag ggcaaggcgt ggctcaccgc gcacgccgcg cagtgggacc
     1441 tggccatcgt cgatctgttt ctgcgccagg gcagcggcct gggcgtgctg gccgcatgcc
     1501 gcgaccgcgc aagcgagcag aagatggtgg tgctcagcaa ctacgccacg cccgacgtgc
     1561 gcatgcgctg cgcgcagctc ggcgtggacg cggtgttcga caagtccaac gagatcgatg
     1621 cgctggtgga ctactgcatc gtgcatggca cgcagcgcgc ggccgccgcc ggggcaccgg
     1681 tcaatcgatc agcctgttct tgagcgcgta ataggtgagg tcgctgttgg acgacagccc
     1741 catcttctcc atgagccgcg tgcggtaggt gctcacggtc ttgacgctga gcgacaggga
     1801 cttggcgatg tccccggccg tctcgccccg ggcgagcttg aggaacacct ggaactcgcg
     1861 ctctgacagc tgctcgtgcg gcggcgcgtc gcccttgcgg ttgagctgct gcgccagcag
     1921 ctcggccacg gccggcgtca aataacggcg ccccagggca atggcacgga tggcctcgac
     1981 gatctccttg gggtcgcatt ccttgttgag gtagccgctc gcgccctggc ggatcaagtt
     2041 gatggcgtaa tgctcctccg ggtagccgct caggatcagg atgcccatgt ccggcgcctt
     2101 ggcgcgcagc atggccagcg catccaggcc gctttgcccg ggcatggaca ggtccatcac
     2161 cagcacgtcg atctcctgcg cgcgcaccag gtcgatggcc tcgcggccat tcgcggcctc
     2221 gcccaccacg cgcaggtcca cgtgctcgga gaaaaactgc cgcagaccgg aacgcactat
     2281 cgcgtggtca tccacaatgc cgatcttgat catctaaccc tctttcagga ttgccggacg
     2341 cggtgccaac catgacacct cagtaacgtt acagggtgat gatatgcaat tcggcccacc
     2401 accaccctgg agaagctcga acatgatctg gtcccgcttg cgcaagatgg ccatcagcct
     2461 gccggcagcc ctgctggcgg tggtcctgct ggtcggcatc aacgagaccg ggtacatgcg
     2521 ctcgcacgat gccgtcgggg acttggcccg ctcgcacgcc acacgcaccg cgctggaccg
     2581 gctgctgcag aacatgctga acgccgagac cggcctgcgc ggctacctcc tgaccggaga
     2641 cgaccgctac ctgcagccct acaacgaggc cgtggccacg atcaactcca acctcgacga
     2701 gctgcgccag acctaccagc ggctgccgca ggaccaggcc gacttcgcgc agctgtcgca
     2761 gcagatcgcg cgcaagctgt ccgagctgga cctgaccctg aacctgcggc gccagaacaa
     2821 cgacgacgcc tggaagttcg ttctgtccac cgacgtgggc aaggacaaca tggacgccat
     2881 acgcgccatt gccgcccgcc tggtcgagcg cagcgcgcaa caggcgcagg gcagcacgca
     2941 ggaaatcctg cactccctga tgctctcgcg catcggcatc gccaccgtgg ccgtgatcgg
     3001 cctgctggcc ttctacatgt acctgcgcca gaccaatgcg ctgcaggagg ccaacgagcg
     3061 cgaacaagag gtgctggcgc gcgagcgcga ccgcctggag cagctggtgc gcgagcgcac
     3121 ggcctcgctg tcggcactgg ccaaccacct gcagcaggtg cgcgaggacg aacgcgcgca
     3181 cctcgcgcgc gaactgcacg acgagctcgg cgcgctgctc acggccgcca agctggacgt
     3241 ggcccggctc aagtccagga tcgacatgca ggtgcccgag catgccgagc gcatccagca
     3301 cctcacggaa acgctcaaca acggcatcgc cctcaagcgc cgcatcatcg agaacctgcg
     3361 cccgtcctcg ctgtccaacc tgggcctgac ggcctcgctg gaaatcctga cgcgcgactt
     3421 cgcccagagc agcaacgtgc aggtggagat cagcctggag gaggctcacc tgtccgacac
     3481 ggcgcagctc accgtctacc gcctggtgca ggagtcgctc accaacatcg gcaagtacgc
     3541 caacgcccgc aaggtgctgg tgtgcgtgca caaccacacc aaccacgtct cggtgcaggt
     3601 acgcgacgac ggcgcgggct tcgacaccac gcgcatccgc gccgacgccc atggcctgct
     3661 gggcatgcgc caccgggtcg aggccgcggg cgggagtctc accgtgagct ccacccccgg
     3721 cgagggcacc ctggtcagcg ccgtgctgcc ccaccggatc gcctgagccg gttcggcggc
     3781 gtcctacacc agcgcacgcc cgccacctgc ccccgcgcgg cgactcccac acttagagtt
     3841 caggtatgcg ctccggcgca tgcacaaacc gcgcggcgcc atgcgcccgt gcacaaaagg
     3901 agttgctcat gttgcactac gcagtggtat tcctggtcat cgccctggtg gccgcagtct
     3961 tcggcttcgg cggcatcgcc gcaggcgccg tgggcatcgc caaggtcctg ttcttcgtct
     4021 tcgtgatcat ggccgtggtg agcttcatca tcggcctgct gaagaagaac taggaccgaa
     4081 cccgcatcgc tcgcaggcag acgcctgtgc ctcgcatctt ccctgaaagg acgcattcgt
     4141 gacagttcag accacgacca agaaagcatt ggagacggcg caccaggccg cagacgacat
     4201 cctcgacggc gccagcgata ccgtgcagtc cgcacgcgac atggccaacc actcgctgga
     4261 caaggccgac agccgcatac accacctgcg ccaggacatg gacccgcgga tagacgacat
     4321 cgcggccaag gcccaggacc tcgccagccg cggcatcgcc tactgcgcca acacgagcga
     4381 ccgggcccgc cgccagctcc ggcaggcggc cgacaccacc acccgctacg tgtccgagaa
     4441 gcccggcacc tcgctgctga tcgccgctgc cgctggcgca gccttcgcca ccgccgtcat
     4501 gctggcgcgc cgcagcggcg agcggcactg aacgggcccg ccatcacggc gcaacaggcg
     4561 ccagccgatc gggcagatca caaccccacg cggccaggag ggtgcaacag gccgcgaggg
     4621 attcctccaa gcacccgagt catcactcca taacccgaaa ggacacaaca tgaaatacgc
     4681 cagcgccatc gccttcgccc tgctgacggg tgccaccatc gtctccaccg gctgctcagt
     4741 ggcccgggac cagcagacgg tgggctccta tgtggacgac gccgccatca ccacggccgt
     4801 gaaggccaag atggccgagg acaagggcgt ttcagccgcc gccatcagcg tcgagacgct
     4861 caacggcacc gtccagctgt cgggcttcgc caagtcgcaa gccgagaagg accgcgccga
     4921 gtccatcgcg cgcaacacca agcatgtgcg cgacgtgcgc aacagcatcg tcgtgcggcc
     4981 ttgatgtaaa caaccccctg agcggctgcg ccgcttcccc cttctctcgc atcgctgcgc
     5041 gctgcgggaa gggggacgcc accagcgcgg cggggcggcc cttgcgcggt ggctctggct
     5101 tgggttgcgc cggtttcata gggtgcgtat cacacgacac gcggtgagaa ccctatacac
     5161 cgattacagg tggcgctttt ttatgccttg gccacccggg cctgcaggcg ccgcagcacg
     5221 gcgggcgaga caaactgcgc cacgtcgccg cccagctggg cgatctcgcg caccagggtg
     5281 ctgctgatga actggtactg gctggcgggg ctgaggaaca ccgtctcgat ctgcggcacc
     5341 agggcgcggt tcatgccggc gagctggaac tcgtagtcga agtcggtgcc cgagcgcagg
     5401 ccgcgcacca tggccgtgcc gccgcgcgcg gcggtgaatt cggtcaccag gccctcgaag
     5461 ggctcgacct gcacctgcgg gcagtcggcc agcgcctcgc gcgccatgtc catgcgctcg
     5521 tccaggctga acatggtttt tttatggtgg gcaatggcca cggccacgat gacgcggtcg
     5581 aacatccgcg ccgcgcgccg cagcaggtct tcatggccca gggtgatggg gtcgaaggtt
     5641 ccgggataga cggcggtgat ctgcgccatg gcgtgggctt ccttgcagtg actggcgccc
     5701 ggtgcgggcc ccgatggggc ggattatgca gcgccgcgag gcgggcagaa ttaacaagcc
     5761 aaatcggtct ctggcgcttt atacgcaagc gccaacagct catttattaa tagcattcac
     5821 tgccgccgca gcaggtgcgc atgcacggcg ccggccttca ggtggcggtg cagcaccagg
     5881 cccagggggt tcagctcggc atccgtccag gcgctgggcg cctccaggta aatgaagccg
     5941 tcgggcgcca cggcctgggc tgcggcctgc agcgccgggg caaacagcgc gccgtcgaac
     6001 ggcgggtcga tcagcagcag ctgcatgctg gccggcgcgc actggcgcag cgcggccacg
     6061 ccgtcgtcgc gctgcacgcg cacggccgtg gcctgcaggc gttggcgcag cagctccagt
     6121 tgccggacaa gtcccgcgtc accctccacc agcaacacac tggccgcgcc gcgcgaggcg
     6181 cattcaaagc ccagggcgcc ggtgccggca aaggcgtcca ggcagcgcca gccggtcagg
     6241 tcctggccca gccagttgaa cagggtctca cgcacgcggt ccggcgtggg acgcaggccg
     6301 gggcgctcgg ccacgggcag gcgcgtgcgc ttccattggc cgccgatgat gcgcacctcg
     6361 cccgcaccgc gcgcggctgc cggcttgggc gcgggctttg gcccgggctt ggcgcgcctg
     6421 gccgggccgc tcaaggctgg cccccgacga tcaccgtggc catgcgcgcg ggctgcaggt
     6481 ggcgctgcat ggcggcgcgc acctcggcca ggctgagggc gttgacgcgc gccgtccaat
     6541 gctccagata gtccagcggc aggtcgttcc aggcgatatt ggccacgttg gccagcagct
     6601 tgcggttgct gtctatgcgc agcgcaaagc cgcctatcag gttgtccttg gcggcgcgca
     6661 gctcggcctc ggtggggccg tcggccacgt agcgcgccag cgtgtcatgc gccacctgca
     6721 cggcttcggc cgcctggtcc gggcgcgtct gcaggccgat gacgaagccg ccccggttca
     6781 gccccggcga gaagtcgctg tatacgctgt aggacaggcc gcgcttttcg cgcacctcct
     6841 gcgtcaggcg cgaggtaaaa cccccgccgc ccaggatatg gttgcccacc agcagggcca
     6901 gaaagtccgg gtcgcgccgc gcaatgccca gctggccgat cagcacctgg gcctgggcgg
     6961 cggcgaaggg gatgtcctcg cgcacggcct tttgcagcgg ctgggcatcg ggcacggcgg
     7021 gcagcggcgc gcagtcggca gcgccgcgcg cgggtaggcg cgccagcagc cgctgcacca
     7081 gggcctgggc ctggacgcgg tcgagcgcgc cgacgatgct cacgcgcgcg cggcaggcgg
     7141 ccacgttgcg ctcatggaag gcctgcaggt ctggcacttc aatgcgcgcc aatgtctctt
     7201 cgctgggctt ctggccatag gggtggtcgc cataaatggc gctgccaaag gccttggccg
     7261 ccaccgggcc ggggcgggtg taggactcct tgatggccgc gctccagcgc gcgcgctcgc
     7321 tggcccacac ggcgggcgga aagctgggct gggcgatctg gcgcgcggcc agctgggcgg
     7381 cgcgctccag caggtcgggc tcggtgagcg agcgcagcgc gtactggaag ctgtcgcgcc
     7441 cggcctgggc gccaaagctc gcgcccagat cggcccaggc ctcgcccagg tcgttttcgt
     7501 ccatggcggc ctcgttggcc gcgccggccg ccacgccctt ggcgctcatc agcgcggcgg
     7561 cggcggccag gccggcctgg ggcgcggggt cgcggcgcga gccggcatca aagtcgatct
     7621 gcacatcgac catgggaatg gccgggctgt gcaccagcca gacgcgcgcg ccgctggcgt
     7681 ccgtccagtg ctgtatcggc agcagcgccc aggcgttttg caagcaaaac aaggctccag
     7741 cgcttaccag taaagcgcga gcagctatct ttttcataga aatattcatg attcgccgcc
     7801 ccggtcagtg catgcgtgtg ccatcggcgg gcttgggtgc ggcgcgcttg gcgcctgccg
     7861 ccagcggctg gggcaagagc gtggccacgg tgagctggtc gtcgccaaag tagcgcgcgg
     7921 cgacggcctg cacctgctcg ggcgtgacgg cacgcagcag cctcaaaagg cgttcgtcgg
     7981 cgtccagcgg caggccctgc acccagttgc tgcccagatc gctggcctgg ctgtagagcg
     8041 agtcgcgcgc gtagatggtg gaggccgccc actgcgtctt cacgcgcgag agctcggcct
     8101 cgctcacgcc ttcgcgtgcc acgcgctcga gctgcgcacg cagcgctgtc tcgacttggg
     8161 ctgccgtctt gccggccgcc ggcacgccgg tcagcatgaa cagcgccggc ccgcggccga
     8221 acaccgaggc atggctgtca gcgctgtcgg ccacgcgctc ggcgccttgc gtcagcgccc
     8281 gctccaggcg cgcgccgtca tagccgctga gcacggctga cagcaccatc agggccaggc
     8341 cgtcgcggtc gccgtcctgc atgtcctcga tgcgctccac gcccggcaca tggaaggcca
     8401 gggccacata ggcctgctcg gccggcgcct tgaagtcgat gcggcgcagg cccttttgct
     8461 cgggctcggt gcggggcttg cgcgccggca gcgcgcgcgc gggaatggcg ccataggtct
     8521 tttgcgccag cgccagcacc tgctgcgggt ccacgtcgcc cgcgaccacc acggcggcat
     8581 tgcccggcgc gtaccactgg cggtagaact ggcgcgcgtc gttggcggtc agagagtcca
     8641 ggtcgctcat ccagcccacc accgggcggc ggtagggcga ggcgatgaag gtggcggcgt
     8701 acagctgctc gatcagcgcg gcgcgcggct gatcctcggt gcgcatgcgg cgctcctcct
     8761 tgaccaccgc cagctccttg gtgaactcgg cgtccggcca ctggttatgg gcaaagcggt
     8821 cggactccag ccgcatcacg tcggccagcc gattggccgg aatctgctgg tagtagccgg
     8881 tgtagtcgcg gctggtgaag gcgttctcct ggccgcccag agccgcgaca cggcgcgaga
     8941 actcgccggg cgccagcttc ttcgtgccct tgaacatcat gtgctcgagc acatgggcca
     9001 cgcccgagcg gccatcgacc tcgtccatgg agcccacgcg cagccacacc atgtgcatgg
     9061 ccgtgggcgc gcgccggtcc ggctgcacga tgagctgcat gccgtttttc agcgtgaaca
     9121 gctgcgcgcc gctggccgtg ctggcctggg ccagcagcgc cggcggcgcc gccgcgggtg
     9181 gaacattttg tgccggggtt tgcgctgcgg cagacaggca ggccagcagg cctaggaggg
     9241 ggatggcgcg tttcatagaa tggctgcgat tctaagaacc cnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn ncaggcctag gagggggatg gcgcgtttca tagaatggct
     9421 gcgattctaa gaacccagcc aatgttcagt tttttcaaga aaaaatccac tgcgcccgcc
     9481 gaggcgccca tcgagacgcc cgccgcgccg ccgccagaca ccgagaccag cgccagcggc
     9541 ggcgccatgg gctggctgcg caagtccttt ggcgcagcgc ccgctgaccg tgaggcgccc
     9601 gccgaccagg aagcaccggc cctgccggcc accaccgaca gcacgccgcc caccgtcgcc
     9661 ctgcccaccc ccaagcccga gggcgagcgc cagggttgga tggagcgcct caaatccggc
     9721 ctgcgcaaga ccggcagctc gattgccacg gtcttcaccg gcacgcagat caacgacgcg
     9781 ctgtacgagg agctggagga tgcgctgctg atggccgaca ccggcgtcaa ggccacgcag
     9841 cacctgctcg atgacctgaa gcgccgcgtg aaggacagca agacgacaga gcccgccgcc
     9901 gtcaaggccc tgctggccga tgccctggca gacctgctca agccgctgga aaagccactg
     9961 gtcataggcg agcaccagcc cacggtgatc atggtggcgg gcgtgaacgg cgcgggcaag
    10021 actacgtcga taggcaagct caccaagcat ctggccgacc atggcgagac ggtgctgctg
    10081 gccgcggccg acaccttccg cgcggcagcg cgcgagcagc tgggcgtatg ggccacgcgc
    10141 aacaccgtgg aaatcatcag ccaggagggc ggcgaccccg ccgccgtgag ttttgacgcc
    10201 gtcacagccg gccgcgcgcg cggcaaggac gtggtgctgg tggacaccgc cgggcgcctg
    10261 cccacacagc tgcacctgat ggaggagctt aaaaaaatcc gccgcgtggt caccaaggcc
    10321 gaggccagcg ccccgcacga ggtgctgttg gtaatcgacg ggaacaccgg ccagaacgcc
    10381 ctgacccagg tgcgcacctt tgacgacgca ctgcaactca ccggcctgat cgtcaccaag
    10441 ctggacggca cggccaaggg cggcgtgctc gcggccatcg cccaggagcg gccggtgccg
    10501 gtctatttca tcggcgtggg cgagaaggtg gaagacctgg aaaccttcaa cgcacgcgag
    10561 tttgcgcagg cgctgctggc ctgactcacc cgtccacctg ctgcgccacc cagcctgcgt
    10621 agtcggcctc ggcctctacg gtgtgggtca gcagctgcgg cacctgatac gggtgctgcg
    10681 cgcgcagcca gtcgcacagc gcgcctgccg cggcgggcag ggtcttgcac accaggcgcc
    10741 attcggcgtc ctcctgctgg gcgccctgcc agacatagtg cgaggcgatg gcctcgacct
    10801 gcacgcaggc cgccaggcgc gagcgcaccg cgccggccgc caggcgctgc gcgtcggccg
    10861 cgctggccac ggtggtggtc acgacggcaa gcgggtgtgc ggcgcctggc atgggttcac
    10921 cggcttattt cttgaccagg tagcagctgc tcttggccag cggctggaag gccttttcgc
    10981 cggcaatggt gtccagcacc ttgtagtagt cccatgggcc cttggactcc ttgggcgact
    11041 tgacctgcac caggaacatg tcgtgcacca tgcggccgtc ttcgcgcaca tagccgtttt
    11101 gcgagaagac gtcgttgatc ttcatcttct tcatctggcc ggccaccgcc aggccgtcgt
    11161 cggtctgggc ctgctccacg gccttcaggt aggtggtcac ggccgagtag atgctggcct
    11221 ggttcatgga gggcattttc ttcatgcgcg cctggtattt ttgcgcccag gcgcgtgact
    11281 catcattgcg atcccagtaa tacgccgtgg caaacaccag gccctggccc tgctccagcc
    11341 ccaggccatg gacgtcgttg atatacatca gctgcggcac gatggtggtc gtcttgttca
    11401 tgccgaactc gcgcgcgccc ttgatggtgt tgatggaatc gttcgtggca ttggccaggg
    11461 ccaccacctg ggccttcgat gcctgggcct gcatcagaaa ggacgagaaa tcaccggcgt
    11521 tgaacgggtg gtagaccgtg cccagcacac ggcccccgcc ctccttgacg aagttggtga
    11581 tgatctccga ggtcaccttg ccgtaggaat agtccacggc aatgatgaac cagtccttct
    11641 tgccctgctt gagcaccgcg tccacggcgg cgctggcaat cgagtacgaa tccaccgtgt
    11701 agtggatcgc cgtgggggcg caaaactcgt tcgtcagcgc gttcgacagg gctcccgtgg
    11761 caatggcgat cttgttgcgc tccttggcca gaacgttcac ggccgaggcg atggccgagt
    11821 tgttcaggtc gatgatcatg tccacgccct cggcgtccca ccattggcgc gccttggcgc
    11881 cgccgatgtc ggccttgttc tggtggtcgg caaacaccac ctccaccggc ttgccgagca
    11941 ccttggcgcc aaagtcctcc acggccatcc gcgcggcctc caccgaaccg cggccgcccg
    12001 agtcggagta ctggcccgac atgtcgctca gcacgccaat cctgaccctg tcgtcggaaa
    12061 taccagcggc atttgcgcca aataccatca aaacccaggt agttgttgcg gctacagcta
    12121 tattttttga aatcatccag gtctcctttg ttgtcgtttg caatggccag gcaaggtttc
    12181 aggcgccctc tggcgtcaga atctgccgcc ccacggcctt gccctcctcc agcgccagca
    12241 gcgccgtgaa ggccttgctg aaatgcatgc aggtggtggg cacgctctgc aattgcttgt
    12301 ccttgaccag ttgcatcagc tcctgcagct cgcgcaggtt gcccacgtag gtgccctggt
    12361 aggtgatggc gcgcagggcc atcgatggcg tgggcaggtc gatatgaccg ccaaacaggc
    12421 cgatctgcac cagctgcccc cccttggtca gcatgtcgat gccggcctgc acggtctggc
    12481 tggagcccac acagtcgatg acggcccaga cggcgccacc ggcggcctgg cgcacctggg
    12541 ccagatagcc gtcctgccgg gggttgaaca cggccagggc gccggcctgc atggctgcgg
    12601 catgcttggt ctcgtcgggc tcgataacga tcacgccggt actgcccatg gccttggcaa
    12661 ggccgatggc catcaagccc aggccgccag cgccaatgag gacgagcttt tcatcgcgca
    12721 gcacctgggg cgggattttc ttcaacgcgc tgtaggtggt caggcccgag cacgcatagg
    12781 gcgcggcctg caccggcggc atgtcgccaa tgtccaccag gtagcgcgcg tgcgacacca
    12841 gcacatggtc ggcatagccg ccgggctgga acacgcccat ggacttgccg gccaggcaca
    12901 ggttttcgtc gccacgcttg catacgctgc actcgccgca gccatgccag gggaagacca
    12961 cgtagcgctt gccgacggtg accccctgca cggcgtcgcc cacggcgatg acctcgccgg
    13021 cgatctcgtg gcccatggtc agcggcagct tcaggccacg gtcggccatg gacagcttct
    13081 tgccatggcc caggtcgtag gagccatgcc agatgtgcag gtcggaatgg cacacacccg
    13141 cggcctcgat gcgcaccagc acctggtcgc ccaccggccg gggcgtggca cgctcgctct
    13201 tgaccagggg ctggccgcac tcgatcacgt catagctgat catcttgtct cctttggttg
    13261 tcataagggg ctaacggcac agtttatggc cgtgaaagca ggttgcaccg ccaccagtca
    13321 cgtccgcgac acgggacatt cccccatgac aagcgacgcg agatctttcc catacaggcc
    13381 gtggaacttt ggctttagca ctcgatcaca gagagtgcta acatgaaccc catgcaagaa
    13441 aggacttctg atatggctct ccagtctgca tccgccgtga cgaccctggc acccgcgaac
    13501 ccgtgggcgc tggtgccacc gctgggcaat ctggatgcgt atatctccgc cgtcaaccgc
    13561 ctgccgctgt tgacgctcga agaagagcag cgctttgcac gccagctcaa ggacgacaac
    13621 gatgtcgagg ctgccgggcg cctggtgatg tcgcacctgc ggctggtggt atctatctcg
    13681 cgccagtacc tgggctatgg cctgccgcat ggcgacctga tccaggaggg caacgtgggc
    13741 ctgatgaagg ccgtcaagcg cttcgacccc gaccagggcg tgcgcctggt gagctacgcc
    13801 atgcactgga tcaaggccga gatccacgag tacatcctga agaactggcg catggtcaag
    13861 gtggcgacca ccaaggccca gcgcaagctg ttcttcaacc tgcgctccat gaagcatggc
    13921 ttcaaggccg acgccattga cgccgacacg caccgcgaga ccctgtccga gcacgagatt
    13981 gatctggtgg ccgagcagct caacgtcaag cgcgaagagg tcatcaagat ggaaacgcgc
    14041 atggccggcg gcgatgtgct gctcgatccc gcacccagtg acgacggcga gcaggccttt
    14101 ggccccattg cctacctggc cgacgcggcc catgaaccca cggccatgat cgagtcgcgc
    14161 cagcgcgacc agctggccac cgacggcatt gccactgcgc ttgcaagcct ggacgcgcgc
    14221 agccgccgca tcgtcgagca gcgctggctg caggtgaacg acgatggctc tggcggcatg
    14281 acgctgcacg aactggccgc cgaatacggc gtgagcgccg agcgcatccg ccagatcgag
    14341 gtggcggcga tgaagaagat gaagcaggcg ctggtcgaat acgcctgagc aacttgccgc
    14401 cacggcagat aatccaggcg cgcggcccac acgggccgcg cgcttttttt gttccaggag
    14461 tattcccatg tttcgctact actccacccg ccgcaacctg ctggcactgg ctctggcgac
    14521 ggtctgcggc tccgccgggg ccgccgacaa gggcgctgcg ccggaaatgc cctcctggcc
    14581 taccaagccc ctgcgcatcg tcgtgggctt tccgccgggc tcctcccccg acctgacggc
    14641 gcgcagcttt gccgaaccgc tggcccaggc cctgggccag ccgctgatcg tggagaacaa
    14701 ggtgggcgcg ggcggcaaca tagctgcgga cgccgtggcc agggcgcgcg atggccacac
    14761 cataggcctg ttcatcaacg gcaacctgac gattgccagg ctgctcaacc cgcgcctgcc
    14821 ctacgacccc gacaaggatc tggcgccgct gtcgctggtt ggcgtgtcgc cgctggtgct
    14881 ggcggcgccc gtgggcctgg gggatgcgac cagcgacgcg cgcgcgttcc tgcaggccgc
    14941 gcgccaggcc ggcaaccgct ggagctatgg cacgccgggc gtgggcacgg tgggccatct
    15001 gggcaccgag ctgctcaagg cgcgcaccgg catggcgccg gtgcatatcc cctaccccgg
    15061 ctacccgcag gtggcgaccg ccatgctggg cggtcagttg cagatggcac tgctgccacc
    15121 ggccctggcg caggcccagg agcgcgccgg caagctgcgc ctgctgggcg tgacctcggc
    15181 cgggcgcagc gcgctggtgc cccatgtgcc cagcctggcc gaggccgggg tgcgcgactt
    15241 tcagctggaa atctggaacg cctttgccgc tccggccgcc atgccggcgc cggtgcgcgc
    15301 acggctgtcc gccttgatca gcgagatcgc gcgcagcccc gaggtgcggg ccaagctgtt
    15361 ccagcagggc tggcaggccg tgggttcatc cagcgaaggg ctggccaacc gcatgcgtgc
    15421 cgacacggcc gccatgggcc gcgtgatccg cgagcaggcc atccgggcgg aataggcgcg
    15481 ggtcagcgcc cttcatcgag cagcgcgcgc acgtccgcgg ccaggctttg cgcacctgcg
    15541 ccgtagcggt ggtagacacg caagcggccc ttggtgtcgt agacatagct gccagccgaa
    15601 tggtccatgg tgtagctcgt cggtgtctgg ccatccacgc gcttgaagta gatcttgaag
    15661 tccttggcca cggccaccag ctgctcgggc gtgccacgca gggcgatgaa gctggggtcg
    15721 aaactggcca tgtaaccctt gagcatctcg ggcgtgtcgc gctcgggatc gacggtgacg
    15781 aagatgccct gcagcttgtc gccgtcggcg cccagcgagc gcttgacctc ggcgagctcc
    15841 gacatcgagg tcgggcacac atccggacat tgggtgtagc caaagaacac caccaccacc
    15901 ttgccggcaa agtccttgat gctgcgctcc ttgccgaact gatcggtcag cggcaggtcg
    15961 cgcccgtact cggcgcccgt cagatccacg ccctggaact tggggccctt tggagaacaa
    16021 gcagaaaaaa tgcctgcagc gcccacccat aaagcgccga tagctatctt tttaagagta
    16081 ttacgtttca acatggttgg aaatcaaaac acatagtgat ccaccagcag cgctgcaaac
    16141 agcaggctca ggtggaccag ggagaagcgg aaggtcttgc gcgccagcgc atccgaatag
    16201 tcgcgccaca gccagtagcc gtacaggcaa aagcccgtgc ccagcagcac ggcggcggcc
    16261 aggtagatcc aggagctcat gccatagaca aagggcatta ggcagccggc aaacaggatc
    16321 agcgtgtaca agaacacctg cagccgcgtg tattcgctgc catgcgtgac cggcagcatg
    16381 ggcaggccgg acttgcggta gtcctccacg cggtacaggg ccagggccca gaaatgcggc
    16441 ggcgtccaca ggaagatgat gaggaacagg atcagcgcct cggcgcccac atcccccgtc
    16501 atggcggccc agcccagcac cggcggcatg gcgcccgagg cgccgccgat gacgatgttc
    16561 tgcggcgtgc gcggcttcag gatcacggtg tagaccaccg cgtagccgac aaaggtggca
    16621 aaggtgagcc acatggtcag cgcattcacc caccagtaca gcagcgccga gccggccgtg
    16681 cacaacaggg ccgagaacgc cagcgcctgc cggttggaca gctcgcccct ggccgtgggg
    16741 cgccaggcgg tgcgtttcat ccgggcgtcg atatgccgct ccaccaggca gttgaaggcc
    16801 gcggccgctc cggccacgag ccagatgccg gcgctggcga tggccatcag cccccactgc
    16861 gcgcggctgg gcaggctggg cacggctagc accatgccga tgaaggcgca gaacacgatg
    16921 agctgcacca cgcgcggctt ggtcagcgcg tagaactgcg ccaggc
//
LOCUS       DAOMTQ010000058        16091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_541_length_16091_cov_25.334123, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000058 DAOMTQ010000000
VERSION     DAOMTQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_541_length_16091_cov_25.334123"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..642
                     /gene="pgsA"
                     /locus_tag="PK518_07575"
     CDS             73..642
                     /gene="pgsA"
                     /locus_tag="PK518_07575"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRM48813.1"
                     /translation="MFFTIPTIMTWTRIVAIPLIVGVFYAPLEPATCNLIATVMFMVF
                     AATDWLDGFLARKLNQASAFGAFLDPVADKFLVCASLLVLVHMQRADVFVALIIIGRE
                     IAISALREWMAQIGAGKSVAVHMLGKLKTTVQMVAIPLLLYDGRLLDIIDTSVWGTWL
                     IWLAAVLTVWSMVYYLQKALPEIRARVAK"
     gene            984..1256
                     /locus_tag="PK518_07580"
     CDS             984..1256
                     /locus_tag="PK518_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRM48814.1"
                     /translation="MNKTELIEHIANNADISKAAAARALESTIEAVKKTLKKGGTVSL
                     VGFGTFAVGKRAARTGRNPRTGATIKIKAAKVPKFRPGKALKDALN"
     gene            1272..1347
                     /locus_tag="PK518_07585"
     tRNA            1272..1347
                     /locus_tag="PK518_07585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1305..1307,aa:Val,seq:tac)
     gene            1407..3329
                     /locus_tag="PK518_07590"
     CDS             1407..3329
                     /locus_tag="PK518_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRM48815.1"
                     /translation="MLESIRKHTKWVMGLLFLLIIPSFVLVGIDQNYFTGGSPVVARV
                     DGKDITQADWDNAHRVESDRVRAQSPDIDAKLLDTPQARYATLERLVRDRVLQTAAQN
                     MHMLTSDVRLARELQGIPQIAALKRPDGSLDAEAYRALAGSQGLTPEGLEARIRHDIS
                     VSQVMGSVMASAFAGTAEAKLALDAILQRREIQVARFNASSFVPNVAVSDADLEAYYK
                     ANPAKFQQPEQASVEYVVLDLDAVKAGITLNEDDLRTYYKENLSRLAGKEERRASHIL
                     IGAAKDAPAAEREKAKAKAMELLAELRKAPGRFAELAKKDSQDPGSAASGGDLGFFGH
                     GAMVKPFEDATFLLKKGEISDVVETDFGYHIIMLTDIKTPRQPSFEELRPTLEAELKQ
                     QQAQRKFAEVAEAFSNAVYEQADSLQPVAEKLKLKIHMADAITRMPVGGAQGALASPR
                     FLEALFQPDSLQNKRNTEAVEIAPSTLAAGRITTYQPAMTLPLDKARDQVRALYVAEN
                     SAKLAREEGQAKLAAWKAAPDSATGLSAPVTVSRDRLQDQPRAVVDSALHAPVDKLPA
                     WVGVDLAAQGYAVIKVNRIVARDAVDDAMARMERQQYLQAWGSAEAQAYYELLKQRFK
                     VQIKAPRPIGQIEPQN"
     gene            3378..3453
                     /locus_tag="PK518_07595"
     tRNA            3378..3453
                     /locus_tag="PK518_07595"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3411..3413,aa:His,seq:gtg)
     gene            complement(3730..4134)
                     /locus_tag="PK518_07600"
     CDS             complement(3730..4134)
                     /locus_tag="PK518_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRM48816.1"
                     /translation="MSDVAEEQVVLAFAKAWNRLEPDGFLALLAPGARYTSQWVFEEL
                     VGAAAISDYLRRKMHTVRARGVNDPDSRVRVEIGRTSHGEDGRPCALMTQGQSDEVKA
                     VVLFEVHDGMVARYDLCIPQLVGDVRTGVFPV"
     gene            complement(4149..5033)
                     /gene="galU"
                     /locus_tag="PK518_07605"
     CDS             complement(4149..5033)
                     /gene="galU"
                     /locus_tag="PK518_07605"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRM48817.1"
                     /translation="MTSTRVRKAVFPVAGLGTRFLPATKASPKEMLPVVDKPLIQYAV
                     EEAYEAGIRDMVFVTGRSKRAIEDHFDTAYELENELEQAGKQAMLELVRSVSPSDMNC
                     LFVRQPRSLGLGHAVLCAEPLVGREPFAVLLADDLMVGENGGPGVMAQMTAAFEKQGR
                     SLLAVQEVPQEHTRRYGIVKGEAAGGPLLRVDQIVEKPAPEVAPSRMGVAGRYVLTPR
                     IFEEIRNQPKGVGGEIQLTDAIARLMAHEAVYAYQYSGKRYDCGSKEGFLEATVELAL
                     QHPEVGASFRQYLQQLSL"
     gene            complement(5082..7952)
                     /locus_tag="PK518_07610"
     CDS             complement(5082..7952)
                     /locus_tag="PK518_07610"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRM48818.1"
                     /translation="MSDTPAQPGLNGLSKSFEPAALEAHWGPEWERRGYGVAGYRGTG
                     QAQAGEPAFAIQLPPPNVTGTLHMGHAFNQTIMDSLTRYHRMLGCNTAWIPGTDHAGI
                     ATQIVVERQLQEQGVSRHDLGRPDFVKKVWEWKEQSGNTITTQMRRLGDTVDWSREYF
                     TMDDKLSKVVTETFVKLYEQGLIYRGKRLVNWDPVLQSAVSDLEVENEEKDGSLWHIA
                     YPLADGSGQLVVATTRPETMLGDVAVMVHPEDERYAHLIGKQVKLPLCDREIPVIADE
                     YVDKAFGTGVVKVTPAHDQNDYQVGQRHKLPMICVLTLTATINDQAPEKYRGMDRFAA
                     RKAVVADLEALGLLVEIKKHKLMVPICTRTGQVIEPMLTDQWFVAMNKVGQGDATGKS
                     IAQKAIDAVASGQVQFVPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEDGKVY
                     VAKSEAEAQAQAPGKTLTRDADVLDTWYSSALVPFSTMGWPNQTDSATDDYNLYLPSS
                     VLVTGYDIIFFWVARMIMMSTHFTGRVPFKHVYIHGLVLDAHGKKMSKSEGNVLDPVD
                     LIDGIELEPLLDKRTQGLRRPETAPQVRKNTQKEFPEGIPAYGADALRFTFAALASLG
                     RSINFDSKRCEGYRNFCNKLWNASRFVLMNCEGFDCGFDGDGSCSESYLHFSQPDRWI
                     VSRLQKVEAEVARGFAEYRLDNAANAIYDFVWNEFCDWYLEIAKVQIQTGSEAQERAT
                     RRTLIRVLEAILRLAHPIIPFVTEALWQVVAPVARIEGASIAIAAYPEAQPAKIDEAA
                     EGYVARLKTMVDACRTLRGEMGVSPAQRLPLLAVADTAEDSAFLRANADVLKNLAKLS
                     DVKVFDDAAAWASEAQNAPVNVVGGARLALFVEIDVAAEKARLSKEATRLQGEITKAN
                     AKLGNEAFCAKAPAAVLEQEKKRLADFGSTLARIQEQLARLG"
     gene            complement(8091..8726)
                     /locus_tag="PK518_07615"
     CDS             complement(8091..8726)
                     /locus_tag="PK518_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48819.1"
                     /translation="MKRPACKPWHQAVLGLVLAALLALPITRHGLEASMWSHMLLQFP
                     LWMLAGALLAAALPPRLRRAIASCNAHGITGLVWVGVLLSVLMVPRVLDLALRDMGVE
                     LAKCTALLLAGVLLRLSWRPAGLVLQGFFLGMLLPMMAVVGQLYIDSPLRLCNAYLLD
                     DQERLGRWLIGLAAVFALAWLAHVGWRLVRREEAALMAAEPQAPKYRASSQ"
     gene            complement(8723..9358)
                     /locus_tag="PK518_07620"
     CDS             complement(8723..9358)
                     /locus_tag="PK518_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRM48820.1"
                     /translation="MLLRTTLASFLLLLGGWAAASWLTYGFQAWTDEGARRLEVALRP
                     VTAPPVTIQGPGLSGSLPALLGEDGGVTIVDFIYTHCETVCLTLGSSFQQLQAALQAD
                     LAAGHDTKLRLLSISFDGARDDPAALQAYAQGLRADPALWRFVRVPDVGEQQALLRAL
                     GVVVIPDGRGDYEHNAALLVFDSRGRMVRIFDLAEQQLAFNYARHLARGPR"
     gene            complement(9362..9952)
                     /locus_tag="PK518_07625"
     CDS             complement(9362..9952)
                     /locus_tag="PK518_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRM48821.1"
                     /translation="MSQDPVTRAQQRENEDPEEAVRPMPVAALLVAAAMVVWAVVYIL
                     STEPLTLSQFGDQRTRAELSGPVAAAGGAVDGKALYAAQCAACHQATGAGLPGVFPPL
                     DGSEWVQGEPRVLANILLHGITGEITVKGNKYQGAMPAFPQLSDAELAGIASFIRGNW
                     SNKAEPLQAELFAKERADGSRTTPFEGEAALKALSP"
     gene            complement(9966..10613)
                     /locus_tag="PK518_07630"
     CDS             complement(9966..10613)
                     /locus_tag="PK518_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HRM48822.1"
                     /translation="MENEAKLTAGAMVTFAIAVSALVILPYVQVRDVKAPEGLKPYTS
                     AELRGRASYIANGCVYCHSQQPRSESFAPDAKRGWGRASVPGDYYYDKPHLLGSMRTG
                     PDLMNIGVRQPSKDWHLGHLYQPRAYVPGSIMPPYPYLFDIKDKAEPGEEAIKLPPGY
                     TPPGKVVVPTPEALDLVRYLQALKRDYPILPPEPQEATGAKTSSVAPAPRPTPAS"
     gene            complement(10631..12262)
                     /locus_tag="PK518_07635"
     CDS             complement(10631..12262)
                     /locus_tag="PK518_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRM48823.1"
                     /translation="MDSAILSLLGAFLLSIIGLFVFIWSLRKGLLVENPRAASAIFAR
                     GEIGHIDDPALGHAGQQRFQAAATEPGDSGHLPDAHEIEDRIAADRSSAFPVFMFIAF
                     ACMWLLFGGIAGLTASLKLHWPDWLVSEAWMTFGRMRTVHLTAVLYGWITNAELAIII
                     WLMPRLLRRPLIGNMWIMMGGALVNVAIASGIGAIGAGWTDGLEYLEMPWQIGIFFAA
                     GMVCIIGPVMYTLVNRKVESLYVTTWYHTAGLLWITLLFIVGKMPGVHFGVQQAAMNW
                     WYGHNVLGLWFTPVAVGAIYYFLPKIIARPIRSYNLSILGFWTLAFFYAQVGGHHLVG
                     GPVPGWLITLSIVQSMMMIIPVLAFSINMGLTMRGRMRLGMYSPTLRFMMFGGFMYLL
                     SSLQGSFEALRSVQQVAHFTHFTVAHAHLGAYGFVTMVLFGAIYFMMPRIMNWEWPYP
                     RLISWHFWLASIGIMIYFVGLTYGGWLQGLVMLDETRPFMDSVAVTIPWLQWRSVGGA
                     LMVASHLVFVFHFLAMAMRFGPNRAGAALFATQQP"
     gene            complement(12515..13783)
                     /locus_tag="PK518_07640"
     CDS             complement(12515..13783)
                     /locus_tag="PK518_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HRM48824.1"
                     /translation="MRAALWFLALFGAAVAVALFAGNNQGTITVFWPPWRVDLSLNMV
                     VLLLLGGFTLLYAALRGLSKLLQMPVRARLWRLQQKERAMHGALLDALSQMLAGRFLR
                     ARKAAESAIAQAGALDDAKHRLPHGTQLRTLAHLVAAESSHALQDKSTREAHLQQALD
                     QIPERAPAPMLELREGAQLRAARWSLDERDAGMALERLATLPAGMARRTLALRARLKA
                     ARMSHQTEQALETARLLGKHHAFSPVAAESIVRGLAIELIGSAHDPAQLQRIWLGLEG
                     AERAMPEIAVHAAQRLAQLDGDAAQVRAWLLPVWERMGELPDAQQLKVVQALQASLDG
                     LDANWLARIESAQRADPRNARLLYLSAMACLQRELWGKAQQLFTQAAQQLADAPLRAS
                     AWRHLAQLAEQRADADAAAHAWKQAALQYP"
     gene            complement(13794..14879)
                     /locus_tag="PK518_07645"
     CDS             complement(13794..14879)
                     /locus_tag="PK518_07645"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRM48825.1"
                     /translation="MSTESAPEPSIAAAAAPQPAPTMGHSGGGVALYALAILTIAALV
                     SSVLLWQRVTNMQEQLARQSADSGTQAVEARTIAQQAQDVARETSARLAVTEARVGEV
                     ALQRGQLEELMQSLSRSRDENLVVDIDAGLRLAQQQAQLTGSLQPLVAALKSASQRIE
                     RAAQPRLAPVLRAMGHDLERLEHASVTDTAGLLVRIDDLVRQVDDLPLINQVAQAAVM
                     RRQAESAPTPAAAASSASESPWQSTLRRAWEGVRDETRGLVRVSRVDHPEAMLIAPDQ
                     AFFLRENLKLKLLNARLGLLARRMDSARADVAATINALNKYFDPASRRTQGAATALQQ
                     LQSHMKTAELPQLDDSFAALATAAAGR"
     gene            complement(14869..15687)
                     /locus_tag="PK518_07650"
     CDS             complement(14869..15687)
                     /locus_tag="PK518_07650"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRM48826.1"
                     /translation="MLSMAPRAIVTRPAREAAQWVADLQARGIAACALPLIAIHPVST
                     PLLLQALHGARQRLPSYRAVMFVSGNAVQYFFDSNLPIALMGQAQTATKTRAWAPGPG
                     TVAALRQAGLPTQHIDAPAADAAQFDSEALWQVVAPQIGPGDRVLIVRGGTTPGAEGG
                     SGREWLSRQIVAAGGAVDFVAAYERGAPQFDADQQALARQAATDGSVWLLSSSEAVAH
                     LTAALPDQHWGQARALATHPRIAQAAQAAGFGSVNECRPAFNEVAASIESLHEH"
     gene            complement(15681..>16091)
                     /locus_tag="PK518_07655"
     CDS             complement(15681..>16091)
                     /locus_tag="PK518_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRM48827.1"
                     /translation="AGLKRLGLHERIHSVFEPDQMLPAAGQGALGLEIRSDRQDLRDA
                     LAPLAHERSWLTVTAERAVSRAMGGSCSMPLAAHGQWSGDQLRLQAAWGDMEGQLPLV
                     RAEGEACVRTLDEADALGLAVAARLRAAGAVAAC"
BASE COUNT         2773 a         5079 c         5333 g         2906 t
ORIGIN      
        1 agagatctac cgtgctttgc gttgaggcga tgctgctgtg aggcatggcg atgcgtgcca
       61 caatcgctac ccatgttttt taccatcccc acgatcatga cctggacgcg catcgtcgcg
      121 attccattga ttgtgggcgt gttctatgcg ccgctggagc ccgctacctg caacctcatc
      181 gctacggtga tgttcatggt gtttgcagcc accgactggc tggatggttt tttggcgcgc
      241 aagctcaatc aggcatcggc cttcggtgcc ttcctcgatc cggttgccga caaatttctg
      301 gtttgcgcat cactgctggt actggtgcac atgcagcgcg ccgatgtatt cgtggctctg
      361 atcatcatcg gcagggagat tgccatcagc gcattgcgtg agtggatggc acagattggc
      421 gcgggcaaga gtgtggcagt gcacatgttg ggcaagctca agaccacggt tcagatggtg
      481 gctattccat tgttgctgta cgacggccgc ctgttggaca tcattgatac cagcgtatgg
      541 ggtacctggc tgatctggtt ggccgcagtg ctgacggtgt ggtccatggt gtactacctg
      601 caaaaggcct tgcccgagat acgtgctcga gtggccaaat aaccgttgca atgggcgtgt
      661 agcggggctc aagcatgcaa gggtatgtcc gcgccccaat agggcatgtc aatacggaat
      721 cggcctagcg acgacgtttg cgagacaaaa cgcttcataa gatccggggt gaggttcaaa
      781 tgcgtctaga attcgccccc atgctgtcgc gtaggcggcg cgttgcattg acagcccgtg
      841 acgctatggt tttacacttc ggtagccact ctgctactgc gagcagcttc cggatttgct
      901 tgggaacctt gagcgccagc gcagaaccgt gaagtgcaga cccacataag acatccaccc
      961 actcataccc cgagaggttt cttgtgaaca aaaccgaact gattgagcac attgccaaca
     1021 acgcagatat ctccaaggcc gccgcagcgc gcgccctgga gtccacgatt gaggcggtca
     1081 agaagacttt gaagaagggc gggacagtgt ccctggttgg ttttggtact tttgcagtag
     1141 gcaagcgtgc tgcacgtacc ggtcgcaatc cccgtactgg tgctacgatt aaaatcaaag
     1201 ctgctaaagt gccaaagttc cgtccaggca aggcattgaa agacgccttg aactgactca
     1261 agaaatatgg ggggtgctta gctcagttgg tagagcggcg cccttacaag gcgtaggtca
     1321 gcggttcgag cccgttagca cccaccaccc cagacaaagg cgaacgcgag ttcgcctttt
     1381 cttttgcagc cgttgaaaga ttcaccatgt tggaatccat acgcaagcac accaagtggg
     1441 tgatgggctt gttgttcttg ttgatcatcc cgtcctttgt cctcgtcggg atagaccaaa
     1501 actactttac tggcggcagc cctgttgtgg cgcgggttga tggcaaggac atcacgcagg
     1561 ctgattggga caacgcccat cgcgtggaaa gcgaccgtgt ccgtgcccaa tctccagaca
     1621 tcgatgccaa gctgcttgat acaccgcagg cgcgctatgc caccctggaa cgcctggtgc
     1681 gcgaccgagt gctgcaaact gccgcgcaga acatgcacat gttgaccagt gacgtacgcc
     1741 tggcgcgtga gctgcaaggc attccccaga ttgcagcttt gaagcggcct gatggctcgc
     1801 tggatgccga ggcgtatcgc gccttggcag gctctcaggg gctcacgccg gaaggcctcg
     1861 aggcgcgcat acgccacgat atctcggtca gccaggtcat gggcagcgtc atggcatcgg
     1921 cattcgctgg cactgccgaa gccaagctgg cactcgatgc gatcctgcag cgtcgggaga
     1981 ttcaggtggc ccgattcaat gcatcatcct ttgtgcccaa cgttgccgtg agcgatgctg
     2041 atttggaggc gtactacaag gccaatcctg caaagtttca gcaacccgag caggccagcg
     2101 tcgaatatgt agtgctggat ctggacgccg tgaaggcagg catcacgctc aatgaagacg
     2161 atctgcgtac ctattacaag gaaaacctca gccgtctggc tggcaaggag gagcgccggg
     2221 ccagtcacat cctgatcggt gcagccaagg acgccccggc tgccgagcga gaaaaggcca
     2281 aggccaaggc catggagttg ctggccgagc tgcgcaaggc gcctggccgc tttgccgaac
     2341 ttgcgaagaa ggactcgcaa gatcccggat cggctgcgtc tggcggcgat ctgggttttt
     2401 ttggccatgg cgccatggtc aaaccgtttg aagatgccac gtttttgctg aagaaaggtg
     2461 agatcagcga tgtcgtggaa accgacttcg gctaccacat catcatgctc acggacatca
     2521 agacaccgcg ccagcccagc ttcgaggaac tgcgccctac gctggaggcc gagctcaagc
     2581 agcagcaggc gcagcgcaag tttgccgagg tggcggaagc gttttccaat gcggtttatg
     2641 agcaggccga cagcctgcaa cccgtggccg aaaagctcaa actcaagatt cacatggctg
     2701 acgccatcac gcgcatgccg gttggtggtg cgcaaggggc gttggccagc cctcgctttc
     2761 tcgaggcgct gtttcagcct gactcactgc agaacaagcg caacaccgag gccgtcgaaa
     2821 ttgcccccag taccttggct gcgggtcgga ttacgaccta tcaaccagcc atgacattgc
     2881 cactggacaa ggcacgtgat caggtgcgag ccctatacgt ggctgagaac tcagcaaaac
     2941 ttgcgcgtga ggaaggtcag gccaagctag ccgcgtggaa ggcagccccc gacagtgcga
     3001 cgggcctgtc tgctccggtc acggtatcac gtgaccgact gcaagaccag ccgcgtgctg
     3061 ttgtcgatag cgccctgcac gcgccggttg acaagctgcc ggcctgggta ggtgtggatc
     3121 tggccgcgca gggttatgcc gtgatcaagg tcaaccgcat cgtggcccgc gacgccgtgg
     3181 acgacgccat ggcgcgcatg gagcgccagc aatacctgca ggcctggggt tctgccgagg
     3241 cacaggcata ctacgaactg ctcaagcaac gcttcaaggt gcaaatcaag gcgccacgtc
     3301 cgattggaca aatcgagcca caaaactgat gatatttccg tttgcataga tttatcgagc
     3361 tataattcaa ggctacggtg gctgtagctc agttggtaga gtccaggatt gtgattcctg
     3421 tcgtcgtggg ttcgagtccc atcagccacc ccaggtaaac aaagaaaacc ctgctattcg
     3481 acagatagcg gggttttttt gttatccggg tttggtagcc accatgtagc cagcgtgact
     3541 gcgtgttgca ccatgtggcc agcgtcccat ctggcatggg ctctcaggaa aagcgcggat
     3601 agaaatcgat tcggcaacaa atcgccgttg gtctggcagc tactccttcg gggtcgcctg
     3661 ctgctgcttg ctctcggctt tggccgtcag ttcgcggtgt gtcttggccg ctgatcaccc
     3721 tgagtacacc taaaccggaa acacacccgt ccgcacatca ccgacgagtt gcgggatgca
     3781 caggtcatag cgtgcaacca tgccgtcatg gacttcaaaa agtacgaccg ctttcacttc
     3841 atcgctctga ccttgcgtca tcaaagcgca aggcctgccg tcttctccgt gactcgtgcg
     3901 gccaatctcc acccgaacac gcgaatccgg gtcattgacg ccacgagcgc gtactgtgtg
     3961 catcttgcgc cgcagatagt ctgaaatagc ggccgcacca accagttctt cgaataccca
     4021 ttgcgacgtg taccgtgctc ccggtgcaag caatgccaga aaaccatccg gctccagacg
     4081 gttccacgcc ttggcgaaag caagaaccac ttgttcttcc gccacatccg acatgccatg
     4141 cactccgatc acagagacag ttgctgcagg tactgccgaa acgacgctcc cacctcgggg
     4201 tgctgtagcg ccagttccac ggtcgcttca agaaaaccct ctttgctacc acagtcatag
     4261 cgttttccgc tgtactggta tgcgtaaacg gcctcatgcg ccatcaggcg ggcgatggcg
     4321 tcggtgagtt ggatctcgcc gcccacgccc ttgggctggt tgcggatttc ttcaaaaatg
     4381 cgcggcgtga gcacatagcg accggccacg cccatgcgcg acggggccac ctcgggggca
     4441 ggtttttcca cgatttgatc gacgcgcaag agcgggccgc cggcggcttc gcccttgacg
     4501 atgccgtagc gcctggtgtg ctcttgcggt acttcctgca cggccaacag ggagcggccc
     4561 tgtttttcaa acgcagccgt catctgcgcc atcacacctg ggccgccgtt ttcgcccacc
     4621 atcaggtcgt cggccagcag cacggcaaag ggctcgcggc cgaccagcgg ctcggcgcag
     4681 agtacggcgt ggcctaagcc caggctgcgc ggctggcgta cgaacaggca gttcatgtcg
     4741 gacgggctga cggagcgcac cagttccagc atggcctgct tgccggcttg ctccagctcg
     4801 ttctccagct cgtaggcggt gtcgaaatgg tcttcgatgg cgcgcttgct gcggccggtg
     4861 acgaagacca tgtcgcggat gccggcctcg taggcttctt ctacggcgta ttggatcaag
     4921 ggcttgtcca ccacgggtag catctctttg ggggacgcct tggtggcggg cagaaagcgg
     4981 gtgcccaggc cggcgacggg gaagacggct ttgcggacgc gggtgctggt catgggggtt
     5041 ccttggtgga gagggccggc gggacgccgg cgctcccggg gtcagcccag gcgggcgagt
     5101 tgttcctgga tgcgcgccag cgtgctgcca aagtcggcca ggcgcttttt ctcttgctcc
     5161 agcacggcgg cgggggcctt ggcgcagaag gcttcgttgc cgagcttggc gttggccttg
     5221 gtgatctcgc cttgcaggcg cgtggcctcc ttgctcaggc gggccttttc cgccgccacg
     5281 tcgatctcga caaacagcgc caggcgcgcg ccgcccacca cgttgaccgg ggcgttctgt
     5341 gcctcgctgg cccaggcggc ggcgtcgtca aacaccttca cgtcgctgag cttggccagg
     5401 tttttcagca catcggcgtt ggcacgcagg aaggcgctgt cctctgccgt gtcggccacg
     5461 gccagaagcg gcaggcgctg cgcgggcgac acgcccatct cgccgcgcag ggtgcggcag
     5521 gcgtccacca tggttttcag gcgcgccaca tagccttcag ccgcctcgtc gatcttggcc
     5581 ggctgcgcct ctgggtaggc ggcgatggcg atcgatgcgc cctcaatgcg ggccacgggc
     5641 gccaccactt gccacagcgc ttcggtgaca aacgggatga tgggatgcgc caggcgcagg
     5701 atggcttcca gcacgcggat cagcgtgcgg cgcgtggcgc gctcttgcgc ctcggagccg
     5761 gtctggatct gcaccttggc gatttccagg taccagtcgc agaactcgtt ccagacgaag
     5821 tcgtagatgg cgttggcggc gttgtccagg cggtattcgg caaagccacg ggccacctca
     5881 gcctccacct tttgcaggcg cgagacgatc cagcgatcgg gctggctgaa atgcagatag
     5941 ctctcgctgc agctgccgtc gccgtcaaag ccgcagtcaa aaccctcgca attcatcagc
     6001 acgaagcggc tggcgttcca gagcttgttg cagaagttgc gataaccctc gcagcgcttg
     6061 ctgtcgaagt tgatgctgcg gcccaaggac gccagcgcgg cgaaggtaaa gcgcagcgcg
     6121 tccgcgccgt aggccggaat gccttcgggg aattcctttt gcgtgttctt gcgcacctgg
     6181 ggtgcggtct cggggcggcg caggccctgg gtgcgcttgt cgagcagggg ttcgagctcg
     6241 atgccgtcga tcagatcgac cgggtcgagc acgttgccct cgctcttgct catcttcttg
     6301 ccgtgcgcgt cgagcaccag gccgtggatg tagacatgct tgaacggcac gcggccggtg
     6361 aagtgggtgc tcatcatgat catccgggcc acccagaaga agatgatgtc gtagcccgtg
     6421 accagcacgc tgctgggcaa atacaggttg tagtcgtcgg tggcgctgtc ggtttgattt
     6481 ggccagccca tggtggaaaa cggcaccagc gcgctggagt accaggtgtc gagcacgtcg
     6541 gcgtcgcgcg tgagtgtttt gccgggggcc tgtgcctgcg cctcggcctc gctcttggcc
     6601 acgtagacct tgccgtcctc gtcgtaccag gccggaatct ggtggcccca ccagagctgg
     6661 cgcgagatgc accagtcctg gatgttgttc atccactggt tgtaggtgtt gacccagttc
     6721 tcgggcacga attgcacctg gccgctggcg acggcgtcaa tcgctttttg cgcgatggat
     6781 ttgccggtgg cgtcgccctg gcccaccttg ttcatggcga caaaccactg atcggtcagc
     6841 atgggttcga tcacctggcc ggtgcgggtg cagatgggca ccatgagctt gtgcttcttg
     6901 atttcgacca agaggcccag cgcttccagg tccgccacga cggccttgcg cgcggcaaag
     6961 cggtccatgc cacggtattt ctcgggcgcc tggtcgttga tggtggccgt cagcgtcagc
     7021 acgcaaatca tcggcagctt gtggcgctgg cctacctgat agtcgttctg gtcatgcgcg
     7081 ggcgtgacct tgaccacgcc ggtgccgaag gccttatcca cgtattcgtc ggcaatcacc
     7141 gggatttcac gatcgcacag cggcagcttg acctgcttgc cgatcaggtg tgcatagcgc
     7201 tcgtcctcgg ggtgcaccat cacggccacg tcgcccagca tggtctcggg gcgggtggtg
     7261 gccacgacca gctggcccga tccatcggcc agggggtagg cgatgtgcca gagtgagccg
     7321 tctttttctt cgttttccac ctccagatcc gacacggccg attgcagcac cggatcccag
     7381 ttgaccaggc gcttgccacg gtagatcagg ccttgttcat acagcttgac gaaggtctcg
     7441 gtcaccacct tggacagctt gtcgtccatg gtgaagtact cgcggctcca atctaccgtg
     7501 tcgcccaggc ggcgcatctg cgtggtgatg gtgttgcccg actgctcctt ccattcccag
     7561 acctttttca caaagtcggg ccggcccaga tcgtggcggc tcacgccttg ttcctgcagc
     7621 tggcgctcta ccacgatctg cgtggcgatg ccggcgtggt ccgtgcccgg aatccacgcc
     7681 gtgttgcagc ccagcatgcg gtggtagcgc gtcaggctgt ccatgatggt ctggttgaac
     7741 gcgtggccca tgtgcagcgt gccggtcacg ttcggcggcg gcagctggat ggcgaaggca
     7801 ggctcgcccg cctgcgcctg gcctgtgccg cgatagccgg cgacgccgta gccacggcgc
     7861 tcccactcgg ggccccagtg ggcctccagg gcggcgggtt caaaggactt ggacaggccg
     7921 ttcaggcccg gttgtgcggg ggtgtcgctc atggcaatga aaagcggcat cccgtgggat
     7981 gccgcttggg tggcagtggt tcggtggggt gcaggaattc tattcgcttg gcgttacgct
     8041 tcgtttttta tagctattgg cgcttgatag taaagcgcaa aggccgtatt tcactgagaa
     8101 cttgcgcggt attttggcgc ctgaggctcg gcggccatca gcgcggcctc ctcccgccgc
     8161 accaggcgcc agcccacatg cgccagccag gccaacgcga atacggccgc caggccgatc
     8221 agccagcggc ccaggcgttc ctggtcgtcc agcaggtagg cgttacacag gcgcagcggc
     8281 gagtcgatgt agagctggcc cacgacggcc atcatgggca gcagcatgcc taggaagaag
     8341 ccctgcagca ccagccccgc cggccgccag gacaggcgca gcagcacgcc tgcgagcagc
     8401 agcgcggtgc atttggcaag ctccacgccc atgtcgcgca gcgccaggtc cagtacgcgc
     8461 ggcaccatca gcaccgacag cagcacgccc acccagacca ggccggtgat gccgtgggcg
     8521 ttgcagctgg cgatggcgcg gcgcaggcgt ggcggcaggg ctgcggccag cagggcgccg
     8581 gccagcatcc acagcggaaa ctgcaacaac atgtggctcc acatgctggc ctccagccca
     8641 tggcgggtga tgggcagcgc caggagggct gccaatacca gtcccaggac ggcctggtgc
     8701 cagggcttgc aggcggggcg cttcatcgcg ggccccgtgc caggtggcgc gcgtagttga
     8761 atgccagctg ctgctcggcc aggtcgaaga tgcgcaccat gcggccccgg gagtcgaaca
     8821 ccagcagcgc ggcgttgtgc tcgtagtcgc cacgaccgtc ggggatcacg accacgccca
     8881 gcgcgcgcag cagcgcctgc tgctcgccca catcgggcac gcgtacgaag cgccacaggg
     8941 ccgggtcggc gcgcaggccc tgggcgtagg cctgcagcgc tgcggggtcg tcgcgcgcgc
     9001 catcaaagct gatggacagc agcctgagct ttgtgtcgtg gcccgccgcc agatccgcct
     9061 gcagcgcggc ctgcagttgc tggaagctgc tgcccagcgt caggcacacg gtttcgcaat
     9121 gggtgtagat gaaatcgaca atggtcacgc cgccatcctc gcccagcagc gccggcaggc
     9181 tgccgctcag gcccggccct tggatggtga cgggtggcgc ggtgacgggg cgcagcgcca
     9241 cctccaggcg gcgcgcaccc tcgtcagtcc aggcctgaaa gccgtaggtc agccagctgg
     9301 ccgcggccca gccgcccagc agcagcagga agctggccag ggtcgtgcgc agcagcatcg
     9361 cttatggcga caaggccttg agcgcggctt cgccctcgaa cggggtggtg cggctgccgt
     9421 ccgcgcgctc cttggcaaac agttcggcct gcagaggttc ggccttgttc gaccagttgc
     9481 cgcggatgaa gctggcgatg ccggccagct cggcgtcgct cagctgggga aaggccggca
     9541 tggcgccctg gtatttgttg cccttgacgg tgatctcgcc ggtgatgccg tgcagcagga
     9601 tgttggccag cacgcgcggc tcgccctgca cccattccga gccatcgagc ggcgggaaca
     9661 cgcccggcag gcccgcgccg gtggcctggt ggcaggcggc gcattgcgcc gcatacagcg
     9721 ccttgccgtc cacggcgccg ccggccgcgg ccacggggcc ggagagctcg gcgcgtgtgc
     9781 gctggtcgcc aaactgcgac agggtgagcg gctcggtgga gaggatgtag accaccgccc
     9841 ataccaccat ggccgcggcc accagcagtg cggccacagg catggggcgg acggcttcct
     9901 cggggtcttc gttctcgcgc tgctgtgcgc gtgtgaccgg gtcttggctc atggtcacag
     9961 tcctttcatg aagcgggcgt gggcctgggg gcgggcgcga cggacgaggt cttggcgccg
    10021 gtggcctctt gcggctcggg tggcaggatg gggtagtcgc gcttgagcgc ctgcaggtag
    10081 cgcaccagat ccagcgcctc gggcgtgggc acgaccacct tgcctggcgg ggtgtagccg
    10141 ggcggcagct tgatggcctc ctcgcccggc tccgccttgt ccttgatgtc gaacagatag
    10201 gggtagggcg gcatgatgga gcctggcaca taggcgcgcg gctggtacag gtggcccaga
    10261 tgccagtcct tgctgggctg acgcacgccg atgttcatca gatcgggccc ggtgcgcatg
    10321 ctgcccagca ggtgtggctt gtcgtagtaa tagtcgcccg gtaccgaggc ccggccccag
    10381 ccgcgcttgg cgtcgggggc aaagctttct gagcgcggct gctggctgtg gcagtagacg
    10441 cagccattgg cgatgtagct ggcgcgcccg cgcagctcgg cgctggtata gggcttgagc
    10501 ccttctggcg ccttcacgtc gcgcacctgg acgtagggca gtatgaccag cgccgagacg
    10561 gcgatggcaa aggtcaccat ggccccggcg gtgagtttgg cttcattttc catgagcttg
    10621 tctcccgggc ttaaggttgt tgggtggcga acagcgccgc gccggcgcgg ttcggaccaa
    10681 agcgcatggc catggccagg aagtggaaga cgaagaccag gtggctggcc accatcagcg
    10741 cgccgcccac gctgcgccac tgcagccagg gaatggtcac ggcgaccgag tccatgaagg
    10801 gtcgcgtttc atccagcatg accaggccct gcagccagcc gccataggtc aggccgacga
    10861 aataaatcat gatgccgatg ctggccagcc agaaatgcca ggaaatcagc cgggggtatg
    10921 gccattccca gttcatgatg cgcggcatca tgaagtagat ggcgccaaac agcaccatgg
    10981 tgacaaagcc gtaggcgccc aggtgggcgt gggccaccgt aaagtgcgtg aaatgcgcca
    11041 cctgctgcac cgagcgcagt gcctcgaacg agccctgcag cgacgacagc aggtacatga
    11101 agccgccaaa catcatgaag cgcagtgttg gcgagtacat ccccaggcgc atgcgcccgc
    11161 gcatggtcag ccccatgttg atggaaaacg ccagcaccgg gatgatcatc atcatgctct
    11221 gcacgatgga cagcgtgatc agccagcccg gcaccggccc acccaccagg tggtggccgc
    11281 cgacctgggc gtagaagaag gccagcgtcc agaagcccag gatggacagg ttgtaggagc
    11341 ggatgggccg cgcaatgatc ttgggcagaa agtagtagat ggcgcccacc gccaccggcg
    11401 tgaaccacag gccgagcacg ttgtggccat accaccagtt catcgccgcc tgctgcacgc
    11461 caaagtgcac gccgggcatc ttgccgacga tgaacaagag cgtgatccac aacaggccgg
    11521 ccgtgtggta ccaggtggtc acatacagcg actcgacctt gcggttgacc agcgtataca
    11581 tcaccgggcc gatgatgcag accatgccgg cggcaaagaa gatgccgatc tgccagggca
    11641 tctccaggta ttccaggccg tccgtccagc ccgcgccgat ggcgccaatc ccgctggcaa
    11701 tggccacgtt gaccagggcc ccgcccatca tgatccacat gttgccgatg agcggccggc
    11761 gcagcaggcg cggcatcaac cagatgatga tggccagctc ggcattggtg atccagccgt
    11821 agagcacggc cgtcaggtgc accgtgcgca tgcgcccgaa ggtcatccag gcctcgctga
    11881 ccagccagtc cggccagtgc agcttgagcg aagcggtgag gcccgcgatg ccgccgaaca
    11941 gcagccacat gcaggcaaag gcgatgaaca tgaagaccgg gaaggcgctg gagcggtcgg
    12001 ccgcgatgcg gtcctcgatc tcgtgcgcgt cgggcaggtg gcccgagtcg ccgggctccg
    12061 tggccgcggc ctggaagcgt tgctggccgg cgtgacccag ggccgggtcg tcgatatggc
    12121 cgatctcgcc gcgcgcaaaa atggccgatg ctgcgcgtgg gttctccacc agcaggccct
    12181 tgcgcagcga ccagatgaag acgaacagtc caatgatgga caacaggaag gcgcccaaca
    12241 ggctcaatat cgcactgtcc atggttggtt ccctttcagt tctggaggca tgtcttctaa
    12301 cccatgggtt gatgtgacac aagtgccata cagcggcacc gtgttatgac gaacggctgc
    12361 gatcataaaa tccatttgaa atgaatgttt attcgtgcgc aagcccatgc tccaagcgtt
    12421 ggtacatggg ggcgacgcgc ggcacagcgc cgtagtgcgc tagcgcgtca gaagggtgat
    12481 tgcgcggacg gcgccgccgt ggggcgcgct gctactacgg gtattgcagc gcggcctgtt
    12541 tccaggcgtg cgcggcggcg tcggcgtcgg cgcgttgctc ggccagctgg gccaggtggc
    12601 gccaggcact ggcgcgcagc ggggcgtcgg caagctgttg cgctgcctgg gtgaacagtt
    12661 gctgcgcctt gccccacagc tcgcgctgca ggcaggccat ggccgacagg tagagcaggc
    12721 gcgcattgcg cggatccgcc cgctgggctg attcgatgcg cgccagccag ttggcgtcca
    12781 ggccgtccag gctggcctgc agcgcctgca ccaccttgag ttgctgcgca tcgggcagct
    12841 cgcccatgcg ctcccacact ggcagcagcc aggcgcgcac ctgggccgcg tcaccgtcca
    12901 gttgcgccag gcgctgggcc gcatgcacgg cgatctccgg catggcgcgc tcggcgcctt
    12961 ccagcccaag ccagatgcgc tgcagctggg ctgggtcatg tgcgctgccg atcaactcga
    13021 tggccaggcc gcgcactatg ctttcagcgg ccaccggcga gaacgcgtgg tgcttgccca
    13081 gcaggcgtgc ggtctccagg gcctgctcgg tctggtgcga catgcgcgcg gccttgagcc
    13141 gggcacgcag cgccagggtg cgccgggcca ttcccgcggg cagggtggcc aggcgctcca
    13201 gcgccatgcc ggcatcgcgt tcgtccagcg accagcgtgc ggcgcgcagt tgcgcgccct
    13261 cgcgcagctc cagcatgggg gccggcgcgc gttcgggaat ctgatccagc gcctgctgca
    13321 gatgtgcctc gcgcgtgctc ttgtcctgca gggcgtgcga gctttcggcg gccaccagat
    13381 gggccagcgt gcgcagttgg gtgccgtggg gcaggcggtg cttggcgtcg tccagcgcgc
    13441 cggcctgggc gatggccgac tctgccgcct tgcgcgcgcg cagaaagcgc ccggccagca
    13501 tttgcgacaa cgcatccagc aacgcaccgt gcatggcccg ctccttctgc tgcaggcgcc
    13561 agagtcgcgc ccgcaccggc atctgcaaca gcttggacag gccgcgcagc gccgcataca
    13621 gcagggtaaa gccgcccagc agcagcagca ccaccatgtt cagcgacaga tccacgcgcc
    13681 agggtggcca gaacacggtg atcgtgccct ggttgttgcc cgcaaacagc gccacggcga
    13741 cggcggcgcc aaacagtgcc aggaaccaga gtgctgcgcg catatgtcgg tgctcagcgg
    13801 cctgccgcag cggtggccag ggcggcgaag ctgtcgtcga gctgcggcag ctcggcggtt
    13861 ttcatgtggg actgcagttg ctgcaatgcc gtggccgcgc cttgcgtgcg ccgcgatgcg
    13921 gggtcgaagt atttgttcaa ggcattgatg gtcgcggcga catcggcgcg cgccgagtcc
    13981 atgcgccgcg ccagcaggcc caggcgtgcg ttgagcagct tgagtttgag gttttcgcgc
    14041 aggaagaagg cctggtcggg tgcaatcagc atggcttcgg ggtgatcaac tcggctcacg
    14101 cgcaccaggc cgcgggtttc gtcacgcacg ccttcccagg cgcggcgcag cgtgctttgc
    14161 cacggcgact cgctggcgct gctggctgct gcggcggggg tgggcgcgct ttcagcctga
    14221 cgacgcatca cggcggcctg ggccacctgg ttgatcagcg gcaggtcgtc cacctggcgc
    14281 accaggtcgt cgatgcgcac cagcaggccc gccgtgtcgg tgacggaggc atgctccagc
    14341 cgctccaggt catggcccat ggcccgcagc accggcgcca ggcgcggctg ggcggcgcgc
    14401 tcgatgcgct ggctggcgct cttgagggcc gccaccagcg gctgcaggct gccggtgagc
    14461 tgggcctgct gctgcgccag gcgcaggccg gcatctatgt ccaccaccag gttttcgtcg
    14521 cgtgagcgcg acagactttg catgagttct tccagctgac cgcgctgcag tgccacctcg
    14581 cccacgcgcg cctcggtgac ggccaaccgt gccgaggttt cacgtgccac atcctgcgcc
    14641 tgctgggcga tggtgcgtgc ctccaccgcc tgcgtgccgg agtcggccga ctgccgcgcc
    14701 agctgctcct gcatattggt cacgcgctgc cacaacagca ccgagctgac cagcgcggcg
    14761 atggtgagga tggccagcgc atacagcgcc acgccgccgc cgctgtggcc catggttggg
    14821 gccggctgtg gggctgccgc tgcggcaatg gacggctcgg gggcggactc agtgctcatg
    14881 aagcgattct atcgacgcgg ctacctcatt gaatgccggg cgacattcat tgacgctgcc
    14941 aaatccggcc gcctgcgccg cctgcgcaat gcgcgggtgc gtggccagtg cgcgggcctg
    15001 gccccagtgc tggtcgggca gggcggcggt cagatgcgcc acggcctccg agctgctcag
    15061 cagccagaca gagccgtcag tggccgcctg gcgcgccagc gcctgctggt cggcatcgaa
    15121 ttgcggcgcg ccgcgctcat aggcggcgac aaagtccacc gcacccccgg cggcgacgat
    15181 ctgccgcgac agccactcgc ggccgctgcc gccttcggcg ccaggtgtgg tgccgccacg
    15241 cacgatgagc acgcggtcgc ccgggccgat ctgcggcgcc acgacctgcc acagcgcctc
    15301 ggagtcgaac tgcgcggcat cggcagcggg tgcgtcgatg tgctgcgtcg gcaggcccgc
    15361 ctggcgcagc gctgccacgg tgccggggcc gggtgcccaa gctcttgttt ttgtagctgt
    15421 ttgcgcttgt cccataagcg ctataggcag atttgaatca aaaaaatact gaacggcatt
    15481 gccactcaca aacatcaccg cgcggtagct gggcaagcgc tggcgcgcgc cgtgcagcgc
    15541 ctggagcagc aagggcgtgc tgactgggtg gatggcgatc agcggcaggg cgcaggccgc
    15601 aatgccgcgc gcctgcaggt cggccaccca ttgcgcggcc tcgcgcgcgg ggcgggtgac
    15661 gatggcgcgc ggcgccatgc tcagcaggcc gccacggcgc cggccgcgcg cagcctggcg
    15721 gccacggcca gacccagggc gtcggcttcg tccagcgtgc gcacacaggc ctcgccctcg
    15781 gcgcgcacca ggggcaactg gccctccatg tcgccccagg cggcctgcag gcgcagctgg
    15841 tcgccgctcc actggccatg tgcggccagg ggcatggagc agctgccgcc catggcacgg
    15901 ctcacggcgc gctcggcggt cacggtgagc cagctgcgct catgggccag tggcgccagc
    15961 gcgtcgcgca gatcctggcg gtcgctgcgt atctctaatc ccaaggcgcc ctggccggcg
    16021 gcgggcagca tctggtcggg ttcaaacacg ctgtggatgc gctcgtgcag gcccaggcgc
    16081 ttgaggcccg c
//
LOCUS       DAOMTQ010000059        15950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_558_length_15950_cov_25.772633, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000059 DAOMTQ010000000
VERSION     DAOMTQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15950
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_558_length_15950_cov_25.772633"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1055
                     /locus_tag="PK518_07660"
     CDS             75..1055
                     /locus_tag="PK518_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48828.1"
                     /translation="MSRTFHRRHWLALAAATLAATGMPAWTQAQNFPAKPITLIVPFP
                     AGGSTDRHMRLLAELAGKQLGQPIIVDNKPGAGGTLAPGAMARTAKADGYTITQFPMS
                     MLRMAHMQKTAWNPLTDFSYIIGVSGYTFGFTVRSDSPYKSFNDYIAAARKSPGAVDY
                     GSTGAGTSPHLLMEELAENAKVKLNHVPFKGNADLQQALLGGHVSAQSDATGWDKYVD
                     GGQMRLLLTFGEKRTQRWPEVPTAKELGYGVVSTSPYGLAGPKGMDPAVVQKLHDAFK
                     KAMDDPRHAELMLQLNQDAWYRSGADFAQWAREAFAKDRLLIERLGLAAK"
     gene            1200..2174
                     /locus_tag="PK518_07665"
     CDS             1200..2174
                     /locus_tag="PK518_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48829.1"
                     /translation="MTNLSRRQWMSHGLGLAGALAAPQWALAQAKYPAKPVRVIVPFA
                     AGGTTDVVARLVLQKLGELMGQSFIVDNKGGANGMIGTAEVARAAPDGYTLLLNTAGA
                     QTLSPVIYKAGYEALASFEPITHICDVSFVVIARKDLPVSNMQELIKLAQGKPLSMSS
                     GSAMINLMSEEFKRLIKAPQTINAQYKGTAPQMQAVVAGEVDFSLDSFASVEMIRAGR
                     VKALGVLSSQRAASFPDVPTLKEQGVDGMEFASWAGLLAPKGTPKDIVDQLAQQMDKV
                     MQMPDVIAKLKSYDYIPHRTSPQAFAKLIDADNKRWQRIVAETGFKIN"
     gene            complement(2237..3265)
                     /locus_tag="PK518_07670"
     CDS             complement(2237..3265)
                     /locus_tag="PK518_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM48830.1"
                     /translation="MKAAFITAHGGNEVVRVRAHHLPERTPGEVRVRMRAATLNQVDL
                     YMRNSGAGITHRLPQIMGLDGAGTIEACDASDPLLHTGQAVVIHPGIACGRCEFCQHG
                     DSALCARIQFLGEHRDGTFAQWAQVPAAQVFPKPAHLNWTEAAALGVNHLTAWRMLFT
                     KARLQPWETVLIFGIGGGVSLAALQLAKAIGARTIVTSRDATKLQRAQAMGADHAIHS
                     TAEDVTKSVMACTDGRGVDVVFENVGAAVWPWAMKSLVRGGRLVTCGATTGDQPGADL
                     RRIFVRQLQIFGSSLGSYGEFRDLLNMVTRTGLRPVIDREFPLEEVRSALDHLETGAQ
                     FGKIALRI"
     gene            complement(3368..4477)
                     /locus_tag="PK518_07675"
     CDS             complement(3368..4477)
                     /locus_tag="PK518_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRM48831.1"
                     /translation="MQRTTMRQTTLPLAGSLVLCCAIGAPAHAQNHPTLYGLIDAGVE
                     RLNNVSTGGSITRMPSIAGSAASRWGLRGSEDLGNGLKAVFTLESGFGSDNGALQQGG
                     RAFGRQAFVGLSGSWGTVSLGRQYSMLFPGAANTDIFLAQIYGAGAFDTYLAGPRLDN
                     ALAYLGKFNGVTLGALYSLGKDVQTCPGERANGSECRAWSAVLKYDAPTWGVGTWIDE
                     QRGYDGSGDTPATADLSGKKDQRLALSGYAMLSQTKLTANYMQRKNDAAANAYRKSSL
                     WSLGVAYPVTQAITLEAQYYHFDYRNSNDGGKMLALRGTYGFTKRTAAYATVGALRNS
                     GNARFSPSVGVSNTAVAPTAGQSQTGIMVGLRHTF"
     gene            complement(4521..5699)
                     /locus_tag="PK518_07680"
     CDS             complement(4521..5699)
                     /locus_tag="PK518_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM48832.1"
                     /translation="MNKSTLLLAGAALATTLPLAALAQAKEPVKIALVTSKSGAFATM
                     GADVATGIKFAVDEANAKGGVDGRKIVLAEGDDESTPDAGRRVAEKLARDGHNLIIGP
                     ITSSISLAISQNLARWDAAFIGTISKADKLTQDACSPRFVRTNHSDAMDLAMINEWAK
                     TIPGNNFAIMAADYVWGQDSAKSFEAAVTGQGKKVGTKLFVPLGTKDYAPYIQQLKNA
                     KADAIWVADVSGAIAFTKQAADFGLIPQTPLIGHALLSNFIVNATGNALTNVPGNVGY
                     TPDIDTPQSKAFAAAFKARHNRLPSDTEGQAYNGTMVLLQGVKLAGSTKPLDVTKALR
                     GAEVETLYGKATVRPADNQLLIPNYIARVKVADGVLRPVIEQSYPATITPPASPLCKL
                     "
     gene            complement(5733..6437)
                     /locus_tag="PK518_07685"
     CDS             complement(5733..6437)
                     /locus_tag="PK518_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48833.1"
                     /translation="MTALLTLEDVDAFYGAAHILHRLSLHVAAGERVALIGRNGVGKT
                     TVVNTILGLAQLRHGTVRIGGEAIPRPRPYLAAQRGVVVVPQGRRIVANLTVEENLLL
                     GAATRRKGPWTVPEIYKLFPILQERAHTPGTALSGGQQQMLAVGRALMSNPSLVLLDE
                     PTEGLAPVIVDQLADIFNRVAGQGTALLLIEQNLSLVARVAHRYLAMAKGAVVAEGTV
                     EATPEGMQALAAHVAV"
     gene            complement(6434..7156)
                     /locus_tag="PK518_07690"
     CDS             complement(6434..7156)
                     /locus_tag="PK518_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM48834.1"
                     /translation="MTAAYVLQAEDVAIHYGGVKALDGVALTLEKGQIRGLIGPNGAG
                     KSTVIDAITGRRRLTRGKVLLDGEDVTALGAVERRRRGLSRSFQRTSIFTGMTVRRQV
                     ELASHQMGMADSDADADAVLQELDLARLSEMIAEDLGYGEQRRLDLALALVGRPKLLM
                     LDEPMAGLSAKESHDLARHLKALTSRWDVSVLLVEHDMDVVFGISDAVTVFELGHVIA
                     SGDPATVRADARVREAYLGSAA"
     gene            complement(7153..8130)
                     /locus_tag="PK518_07695"
     CDS             complement(7153..8130)
                     /locus_tag="PK518_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM48835.1"
                     /translation="MSLSRIGLLSVLALAGGTLTMWLTTSASLLGMFTQAVIYAIFAL
                     GVGVLLRQSGLVSFGHALFFGAAGYGMGVNLELQWMPAELALLVILVAIGVLAFAIGL
                     VIVRVPGIAFGMLTLAIGQMAYLLASRSRGITGGADGMGIAWPNTLFGVQQATLLAPA
                     TLFMLAWTLMVFVTFMLLWLLRTRFGAITEAVRDNEERARFIGIKTTVPRALVYALSA
                     VVTGIAGLLSALNTGFVSPESLHWSVSAITLLMVVVGGFKRAAGPIVGAIVYFLFKDL
                     LGDWATHSMAIFGAALIAVIVFSPDGITGALARLLRRLRHGPSKKGAHP"
     gene            complement(8154..9005)
                     /locus_tag="PK518_07700"
     CDS             complement(8154..9005)
                     /locus_tag="PK518_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM48836.1"
                     /translation="MLTINLFNGLVYGALLIIMSSGLALIYGLRRVVNFAHGALYMLG
                     AYIGFSVATHSNFWVALVAAPAVMALLGVLLDRYGFRLLQDHEPLNVMLVTFGLLLIL
                     EDLVAFIWGKGNHSLFTPDLLNFSVNLFGESVPAYRIGVIFVGAAVAMGLTLWLRYSR
                     IGLFVRAASTDPVTTSMQGVNTDRLGAAVVGLGAALAGLAGVVAAPFLSLSPHMSSDV
                     LIDSFVVVVIGGLGSLAGAFIAALLLGMMQAIGAVYLPQVSVLLPFLFMIAVLIWKPS
                     GLAGSRT"
     gene            complement(9088..9879)
                     /locus_tag="PK518_07705"
     CDS             complement(9088..9879)
                     /locus_tag="PK518_07705"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRM48837.1"
                     /translation="MDKPHDSLRPIQVATHGRVGLITLHRPRQLNALNDALMDKLGAA
                     LKSFDANPGIGAIVITGSDKAFAAGADIDAMADWSYMDVYQGDFITRNWETIRTIRKP
                     VLAAVAGYAMGGGCELALACDIVVAAESARFALPEIKLAMLPGAGGTQRLPRAVGKAK
                     AMDMCLSARTLDAQEADRYGLVSRVVPDAELLARTLALAGQIASYSLPALMAIKESVN
                     RAWESSLTEGILFERRALHARFASTDAHEGMHAFLQKRPPSFIHR"
     gene            complement(9891..11552)
                     /gene="aliA"
                     /locus_tag="PK518_07710"
     CDS             complement(9891..11552)
                     /gene="aliA"
                     /locus_tag="PK518_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HRM48838.1"
                     /translation="MEFDAVLLPPRRARLIAQGLWHDRTINAELDACAAARPQQLALT
                     AVQVETGDVRRFSYGEMAQMADRIAVGLARLGVGRGDVVACQLPNWWQFTLTYLACSR
                     IGAVMNPLMHIFRERELSFMLNHGEAKVMIAPKTFRGFDFEAMVTGLQPSLPHLKHVV
                     IVGGDGGNSFDALLSAPEWEKQADAQDILTRGRPGPDDVTQLIYTSGTTGEPKGVMHS
                     ANTVMANIIPYAERLKLDAEDVVLMASPMAHQTGFMYGLMMPILLKTSVVLQDIWEPK
                     KALSLIQSERVTFTMASTPFLTDLARTVAEDVQAGGPGVPTLRTFLCAGAPIPGPLVQ
                     QARAALGAKIVSAWGMTENGAVTLTKLDDDDERSFNTDGCPLPGVEIKVVDADGASLP
                     SGESGKLLLRACSNFGGYLKRPQLNGTDADDWFDTGDLARIDERGYVRITGRSKDVII
                     RGGENIPVVEIESLLYKHPAVAMAAIVAYPDERLGERACAVVVPKAGQSIDLPEIQRF
                     LKEQQIAVQYMPERLIVQEAMPATPSGKIQKFKLRDAVQELIKSN"
     gene            complement(11687..12844)
                     /gene="aliB"
                     /locus_tag="PK518_07715"
     CDS             complement(11687..12844)
                     /gene="aliB"
                     /locus_tag="PK518_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxyl-CoA dehydrogenase"
                     /protein_id="HRM48839.1"
                     /translation="MNPNPYINQDLQALAETARRFAQERVAPGFLERDRSRTFDRALM
                     REMGELGFIVPELPEQFGGLGLGCLAAGVIHEEIARADLSFSYVNLLASLNSQILSRY
                     GQPEVVTPWLQKIIAGESICAIALTEPRGGSDAANLRLKIERVGDEYVINGEKTSISA
                     ADQADICVVFGRTGTPESGAHGVTALLVPMDTPGVTTNRFDCHGQRAIGRGSIFFENA
                     RVPVNHRLGDENKGFVQVMHGFDFSRSLIGLQCLAVARVALEEAWQYITEREAFGQPL
                     AAFQGVTHPLATYDTEVEGARLLCLQGLWLKDQGLPHSAEAGMAKWWGPKLAYDVIHQ
                     CLLCFGHGGYDRGPMEQRLRDVLGFQIGDGTAQIMKTIVARTRAGRKFVPA"
     gene            complement(12881..13663)
                     /gene="badI"
                     /locus_tag="PK518_07720"
     CDS             complement(12881..13663)
                     /gene="badI"
                     /locus_tag="PK518_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketocyclohexanecarboxyl-CoA hydrolase"
                     /protein_id="HRM48840.1"
                     /translation="MTFEDLLYEVKNGVAWITINRPDKMNAFRGTTCDELIKALNKAG
                     YDKSVGAIVLAGAGDRAFCTGGDQSAHDGNYDGRGTIGLPMEELHTAIRDVPKPVIAR
                     VQGFAIGGGNVLATICDLTICSDKAQFGQVGPKMGSVDPGYGTAFLARVVGEKKAREI
                     WYLNRRYSGAEAVAMGLANFCFPHDQLDAEVQKIGEELCERSPTALAIAKRSFNMDTA
                     HQAGIAGLGMYALKLYYDTDESREGVNALKEKRKPEFRKYVK"
     gene            complement(13697..14464)
                     /gene="badH"
                     /locus_tag="PK518_07725"
     CDS             complement(13697..14464)
                     /gene="badH"
                     /locus_tag="PK518_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycyclohexanecarboxyl-CoA dehydrogenase"
                     /protein_id="HRM48841.1"
                     /translation="MQGLKDETIIVTGGGGGIGSATCRRLAREGAKVAVFDRNLEAAQ
                     KVVAEITAAGGQAAAFDCDITDRASVDAAVAATQAQLGPVAVLVNNAGWDVFKPFTKT
                     VPAEWDRLIAINLTGALHMHHAVLPGMAERKKGRIVNVASDAARVGSSGEAVYAACKG
                     GLVALTKALAREHARHGITLNVVCPGPTDTALLAGVAEGAGNPEKLLEAFTRAIPLGR
                     LGQPDDLASAIAFFASADAGFITGQVLSVSGGLTMNG"
     gene            14597..15103
                     /locus_tag="PK518_07730"
     CDS             14597..15103
                     /locus_tag="PK518_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM48842.1"
                     /translation="MENQYKKVKETRMELANRLFFKLYQCANMLHKTGSRAVESEGLT
                     TQQWAVLGALSTTDEGMSIGALARYLMVSRQNLAGLIGRMERQGHVCSAPDGRDRRSR
                     LVAMTPAGRLTWERGAQAKIEDYYTQALEGFSTSDQVHALHYLLKLLDNMQRIDIAQG
                     ASEEDDAG"
     gene            complement(15139..>15950)
                     /locus_tag="PK518_07735"
     CDS             complement(15139..>15950)
                     /locus_tag="PK518_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM48843.1"
                     /translation="DAGHPLLGAADYKLVHHAQNGQAKGRTVYSFCMCPGGTVVAATS
                     EPGRVVTNGMSQYSRAERNANAGMVVGVEPHDYPRDPAAFEAMLGATHGVEALAADKT
                     HPLAGIVLQRQLEARAFELGGGNYNAPAQRVGDFLARRASTALGEVQPSYQPGVTLVD
                     LHEALPAYATAAMREALPAFARKIRGYDMQDAVLTGVETRTSAPLRIDRGEDFQSLNT
                     EGLYPAGEGAGYAGGILSAGVDGIKVGEAVACAMLGLPVPSSGARGSGGAA"
BASE COUNT         2637 a         5129 c         5396 g         2788 t
ORIGIN      
        1 acgcgctctg agcgcgcgcc accgtaccgg ggcatcgcgt gcgatgccct tttttcagct
       61 tcaaggagga acgcatgagc cgtacattcc accgtcgcca ctggctggcc ctggccgctg
      121 ccacgctggc tgccaccggc atgcccgcct ggacccaggc gcagaacttt ccagccaagc
      181 ccatcacgct gatcgtgccc ttcccggcgg gtggctccac cgaccgccac atgcgcctgc
      241 tggccgagct ggccggcaag cagctgggcc agcccatcat cgtggacaac aagcccggcg
      301 cgggcggaac gctggcgccc ggcgccatgg cgcgcacggc caaggccgac ggctacacca
      361 tcacgcagtt ccccatgtcc atgctgcgca tggcgcatat gcagaagacg gcctggaacc
      421 cgctcaccga cttcagctac atcatcggtg tgtcgggcta cacctttggc ttcacggtgc
      481 gcagcgactc gccctacaag agcttcaacg actacatcgc cgccgcgcgc aaaagccccg
      541 gcgcggtgga ctatggctcc acgggcgcgg gcacttcgcc acacctcttg atggaggaac
      601 tggccgagaa cgccaaggtg aaactcaacc atgtgccgtt caagggcaac gccgacctgc
      661 agcaggcgct gctgggcggc catgtgtcgg cgcaaagcga tgccaccggc tgggacaaat
      721 atgtggacgg cggccagatg cgattgctgc tgacctttgg cgagaagcgc acccagcgct
      781 ggcccgaggt gcccaccgcc aaggagctgg gctatggcgt tgtctccacc tcgccctatg
      841 gcctggccgg ccccaagggc atggacccgg ccgtggtgca gaagctgcac gacgccttca
      901 agaaggccat ggacgacccg cgccacgccg agctgatgct gcagctgaac caggacgcct
      961 ggtaccgctc gggcgcggac tttgcgcaat gggcccgcga ggcctttgcc aaggacaggc
     1021 tgctgatcga gcgcctgggc ctggcagcca agtagccctg gaggcgggcg gggttgctga
     1081 actgcgaccc acaggcatac acaaaagcaa tcgttgcagt cgatttgggt attggacgtg
     1141 gccgccgacc ccgtcccaca atgccgggca caactattca ttcaatggag acaaagcaca
     1201 tgaccaacct ttcccgccgt caatggatga gccacggcct gggcctggcc ggggccctgg
     1261 ctgcgccgca atgggcgctg gcacaggcca agtacccggc caagccggtg cgcgtcatcg
     1321 tgccctttgc ggccggcggc acgaccgacg tggtggcgcg cctggtgctg caaaagctcg
     1381 gcgagctgat gggccagtcc ttcatcgtgg acaacaaggg gggtgccaac ggcatgatcg
     1441 gcacggccga ggtggcgcgc gccgcgcccg atggctacac cctgctgctg aacaccgccg
     1501 gcgcccagac cctgagcccg gtgatctaca aggccggcta cgaggccctg gccagcttcg
     1561 agcccatcac gcatatctgc gatgtgagct tcgtggtgat tgcgcgcaag gatctgccgg
     1621 tgagcaacat gcaggagctg atcaagctgg cgcagggcaa gcctctgtcc atgtcctcgg
     1681 gcagcgccat gatcaacctg atgagcgagg agttcaagcg cctgatcaag gcgccgcaga
     1741 ccatcaacgc ccagtacaag ggcacggcgc cgcagatgca ggccgtggtg gccggcgagg
     1801 tggatttctc gctggactcg tttgcctctg tggagatgat ccgcgccggc cgcgtgaagg
     1861 cgctgggcgt gctgtcgtcg cagcgcgcgg cctcattccc cgacgtgccc acactcaagg
     1921 agcagggcgt ggacggcatg gagttcgcgt cctgggccgg cctgctggcg cccaagggca
     1981 cacccaagga catcgtcgat cagctggccc agcagatgga caaggtcatg cagatgcccg
     2041 atgtgatcgc caagctcaag tcctacgact acattccgca ccgcacctcg ccgcaggcat
     2101 ttgccaagct gatcgatgcc gacaacaagc gctggcagcg catcgtggcg gagacggggt
     2161 tcaagatcaa ttgaggcgtg gggcaccaac ggctccaaac ctatccgata acgacaggtt
     2221 tggagcgtaa ggccgttcag atacgcaagg cgatttttcc gaattgtgcc ccggtttcga
     2281 gatgatcgag ggcactgcgt acctcttcca gtggaaactc cctgtcgatg acgggccgca
     2341 agccggttct agtgaccatg ttcaacaggt cgcgaaattc accgtaactg cccaggctgg
     2401 agccgaatat ttgcaactgc cgcacaaaga tgcggcgcag atcggctccc ggttgatcgc
     2461 ccgtcgtggc gccgcatgtc accaggcgtc cgcctcgtac cagcgatttc atggcccatg
     2521 gccacaccgc cgcgccaacg ttctcgaaaa ccacatccac cccccgtccg tcggtgcagg
     2581 ccatgacgga cttggtcaca tcctcggccg tactgtggat ggcgtggtcg gcacccatgg
     2641 cctgggcacg ctgcagcttg gtagcgtcgc gtgatgtgac tatggtgcga gcccctatgg
     2701 ccttggccag ctgcagtgcc gccaacgaca cgccgccgcc gatgccgaat atcagtaccg
     2761 tctcccacgg ttgcaggcgc gccttggtga acagcatgcg ccaggcagtg aggtgattca
     2821 ctcccagggc tgcggcttcg gtccagttca gatgggctgg cttcggaaat acttgcgccg
     2881 ccggaacttg ggcccattgc gcaaaagtac cgtcccggtg ctctccgagg aattgaatgc
     2941 gtgcgcacaa ggcgctgtcg ccatgctggc agaactcgca gcgaccgcag gcgatgccgg
     3001 ggtggatgac cactgcctgg ccggtgtgca gcagggggtc gctggcgtcg caagcctcga
     3061 tcgtaccggc gccgtccagg cccatgatct gcggcaaccg gtgtgtgatg cctgccccgc
     3121 tattgcgcat gtacagatcc acctggttca gggtggccgc gcgcatgcgc acgcgtactt
     3181 cgccaggtgt gcgctccggc aggtggtgcg cgcgcacccg cacgacttcg ttgccgccgt
     3241 gggcggtgat gaaggctgct ttcatgcgag tccttggtgg cctctctatt tttatagcgt
     3301 actacgctgt tcagataagc gcttccggct ggttatatta ataatcagca ggaagcatgg
     3361 cgcgtgctca gaaggtgtgg cgcagtccga ccatgatgcc ggtctggctt tggcccgcgg
     3421 tgggtgccac cgccgtgttg ctcacgccca ccgatggcga gaagcgtgca ttgccgctgt
     3481 tgcgcagcgc gcccaccgtg gcgtaggcgg ccgtacgctt ggtgaaccca taggttccgc
     3541 gcagcgccag catcttgccg ccgtcgttgc tgttcctgta gtcgaagtgg tagtactgag
     3601 cctccagtgt gatggcctgc gtgaccgggt atgcaacgcc cagcgaccac aggctgctct
     3661 tgcggtacgc attggctgct gcgtcgttct tgcgctgcat gtagttggcc gtcagtttgg
     3721 tctggctgag catggcgtag ccgctcaggg ccaggcgctg atccttcttg ccgctcaggt
     3781 ctgcggtggc gggcgtgtcg ccgctgccgt cgtagccgcg ctgctcgtcg atccaggtgc
     3841 ctacgcccca ggtgggcgcg tcgtatttca gcacggcaga ccaggcgcgg cattcgctgc
     3901 catttgcgcg ttcaccgggg caggtctgca catccttgcc caggctgtag agcgcaccca
     3961 gcgtaacgcc gttgaactta cccaggtagg ccagggcgtt gtccaggcgc gggccggcca
     4021 ggtaggtgtc gaacgcgccg gcgccataga tctgcgccag aaagatgtcg gtgttggcgg
     4081 ctccagggaa gagcatcgag tactggcgcc ccagcgagac cgtgccccag ctgccggaca
     4141 ggccgacgaa ggcctggcgg ccgaaggcgc ggccgccttg ctgtaaggct ccgttgtccg
     4201 agccaaaacc cgattcgagc gtgaacacgg ccttcaggcc attgcccagg tcttcgctgc
     4261 cgcgcagccc ccagcgcgac gcggccgagc cggcgatgct gggcatgcgc gtgatgctgc
     4321 cgccggtact gacgttgttc aggcgttcga cgccggcgtc gatcagcccg tagagggtgg
     4381 ggtggttctg tgcgtgtgcc ggggcgccga tggcgcagca cagcaccagc gagccggcga
     4441 gtggcagagt ggtttgtcgc atcgtggttc gttgcattgc gttttgtctc ctgtgttctt
     4501 ggtggtaggg cattggaggg tcacagcttg cacagcggtg atgccggcgg cgtgatggtg
     4561 gcggggtagc tttgctcgat gacggggcgc agcacaccgt cggccacctt cacgcgcgcg
     4621 atgtagttgg gaatcaagag ctggttgtcc gccgggcgca ccgttgcctt gccgtacagc
     4681 gtctcgacct cggcgccgcg cagggctttt gtcacgtcca ggggcttggt gctgccagcc
     4741 agctttactc cctgcagcag caccatggtg ccgttgtagg cctggccctc ggtgtcggag
     4801 ggcaggcggt tgtgccgggc cttgaaggcc gcggcgaagg ctttgctctg cggcgtgtcg
     4861 atgtccggcg tgtagcccac gttgccgggc acattggtga gtgcgttgcc cgtggcgttg
     4921 acgatgaagt tggacagcag cgcgtggccg atcaggggcg tctgcggaat caggccgaag
     4981 tcggcggcct gcttggtgaa ggcgatggct cccgatacgt cggccaccca gatggcgtcg
     5041 gccttcgcgt tcttgagctg ctggatgtag ggtgcgtagt ccttggtgcc cagtggcacg
     5101 aacagtttgg tgccgacttt cttgccttgg ccggtgacgg cggcctcgaa cgacttggcc
     5161 gagtcctggc cccacacgta gtcggcggcc atgatggcga agttgttgcc ggggatggtc
     5221 ttggcccact cgttgatcat cgccaggtcc atggcgtcgg aatggttggt gcgcacgaag
     5281 cgtgggctgc aggcgtcctg cgtgagcttg tcggccttgc tgatggtgcc gatgaaggcc
     5341 gcgtcccagc gcgccaggtt ttggctgatc gccagcgaga tggacgatgt gatggggccg
     5401 atgatgaggt tgtggccgtc gcgcgcgagc ttctcggcca cgcgccggcc ggcgtcgggc
     5461 gtgctttcgt cgtcgccttc agccagcacg atcttgcggc catcgacgcc gcccttggcg
     5521 ttggcttcat cgacggcgaa cttgatgccg gtggccacgt ccgcgcccat ggtggcgaag
     5581 gcgccggact tggatgtgac cagcgcgatt tttaccggct ccttggcctg ggccagagcc
     5641 gccagaggca gcgtggtggc cagcgcggcg cctgccagca gaagggtgga tttgttcatg
     5701 cttgtctcct tgggttgggg gggtgaaaag tgttaaaccg cgacgtgcgc cgccagggcc
     5761 tgcatgcctt cgggcgtggc ctccaccgtg ccttcggcca ccacggcgcc cttggccatg
     5821 gccaggtagc ggtgcgccac ccgggccacc aggctcaggt tctgctcgat cagcagcagg
     5881 gcagtgccct ggcccgccac gcggttgaag atatcggcca gttgatcgac gatgaccggc
     5941 gccagccctt cggtgggctc gtcgagcagt accaggctgg ggttggacat gagggcgcgg
     6001 cccacggcca gcatttgctg ctgcccgccc gacagggccg tgccgggggt gtgcgcgcgc
     6061 tcctgcagga tggggaacag tttgtagatt tcgggcactg tccaggggcc cttgcggcgc
     6121 gtggcggcgc ccagcagcag gttttcttcc accgtcaggt tggccacgat gcgccgcccc
     6181 tgcggcacca ccaccacgcc gcgttgcgcg gccaggtatg ggcgtggacg cgggatggct
     6241 tccccgccga tgcgcacggt gccgtggcgc agttgcgcca ggccgaggat ggtattgact
     6301 accgtggtct tgcccacgcc gttgcggccg atcagggcca cgcgttcacc ggcggccaca
     6361 tgcaggctca ggcggtgcag gatgtgggcg gcgccgtaga acgcatccac atcttccaat
     6421 gtgagcaagg cagtcatgcg gcactcccca ggtaggcttc gcgcacgcgt gcatcggcgc
     6481 ggacggtggc cgggtcgccg ctggcgatca cgtggccgag ttcgaaaacg gtgacggcgt
     6541 cggagatgcc gaacaccacg tccatgtcgt gttcgaccag cagcaccgac acgtcccagc
     6601 gcgaggtcag cgctttcagg tggcgcgcca ggtcgtgcga ctcctttgcc gacagaccgg
     6661 ccatgggttc gtcgagcatc aacagcttgg ggcggcctac cagagccagg gccagatcca
     6721 gccggcgctg ttcgccgtag cccagatcct ccgcgatcat ttcgctcagc cgcgccagat
     6781 ccagctcttg cagaacggca tcggcgtcgg cgtcgctgtc ggccataccc atctggtgcg
     6841 aggccagttc cacctggcgg cgtaccgtca tgcctgtgaa gatgctcgtg cgctggaagc
     6901 tacgcgacag gccgcgccgt cggcgttcca cggcgcccag tgcggtgacg tcctcgccat
     6961 cgagcaacac cttgccacgc gtcaggcgcc tgcgtccggt gatggcgtcg atcacggtgg
     7021 acttgcccgc gccgttgggg ccgatgaggc ctcgaatctg gcccttctcc agcgtcagcg
     7081 cgacgccgtc gagggccttt acgccaccgt agtggatggc cacgtcttcg gcctggagca
     7141 cgtatgcagc ggtcatgggt gcgcaccttt cttcgaaggt ccatggcgca ggcggcgcag
     7201 cagccgcgcc agcgctcctg tgatgccatc gggcgagaaa acgatcacag cgatcagggc
     7261 ggcgccgaag atcgccatgg aatgcgtggc ccagtcgccc agcaagtcct tgaacaggaa
     7321 gtaaacaatg gctccgacga tggggcccgc ggctctcttg aagccgccaa ccaccaccat
     7381 cagcagcgtg atggccgaga cgctccagtg caggctctcg ggcgagacga agcccgtgtt
     7441 cagcgccgac aacagccccg caatgcccgt gaccacggcg gacagggcgt acaccagggc
     7501 acgcggcacc gtggttttga tgccgatgaa gcgcgcgcgc tcttcgttgt cgcgaaccgc
     7561 ctcggtgatg gcgccaaagc gcgtgcgcaa gagccacagg agcatgaagg tgacgaacac
     7621 catcagcgtc caggccagca tgaacagcgt ggcgggcgcc agcagcgtcg cttgctgcac
     7681 gccgaacagg gtgttcggcc aggcgatgcc catgccgtcg gcgccaccgg tgatgccgcg
     7741 cgagcgcgag gccagcaggt aggccatctg gccgatcgcc agcgtcagca tgccaaaggc
     7801 gatgccaggc acgcgcacga tgacgagacc aatggcgaac gccagcacgc cgatggctac
     7861 cagaatcacc agcagcgcca gttctgcggg catccattgc agctccaggt tcacccccat
     7921 gccgtagccg gccgcaccaa agaacagcgc gtggccaaaa ctgaccaggc cgctttgccg
     7981 cagcagcacg cctacgccga gtgcgaagat ggcgtagatg acggcctggg tgaacatgcc
     8041 aagaaggctt gccgaggtgg tcagccacat cgtcagtgtg ccgcctgcca gggccagcac
     8101 cgacaacagc ccgattcggg aaagactcat gcccatgact gcattccttc cgatcaggta
     8161 cggctgccgg ccagccccga gggcttccag atcagcacgg cgatcatgaa caggaacggc
     8221 agcagtaccg acacctgggg cagatagact gcaccgatgg cctgcatcat ccccagcagg
     8281 agcgcagcga tgaatgcccc ggccagcgag cccaggccgc ctatgacgac caccacgaag
     8341 gagtcgatca gcacgtccga actcatgtgc ggcgacagcg acaggaatgg cgcggccacc
     8401 acgcccgcca gccccgccag ggcagcgccc agaccgacca cggcagcgcc cagccgatcg
     8461 gtattcacgc cttgcatgga ggtggtcacc gggtcggtgc tggcggcgcg cacgaacagg
     8521 ccgatgcgtg agtagcgcag ccacagcgtc agccccatgg ccacagcggc accgacgaag
     8581 atgacgccga tgcgatacgc cggcacgctc tcgccgaaca ggttgaccga gaagttcagc
     8641 agatcgggcg tgaacagcga gtggttgccc ttgccccata tgaaggccac cagatcttcc
     8701 aggatgagca gcagaccgaa ggtgaccagc atcacgttca gcggctcatg gtcttgcagt
     8761 agccgaaacc catagcggtc gagcagtact cccagcaggg ccatgacggc aggtgccgcc
     8821 acgagggcca cccagaaatt gctgtgcgtg gccaccgaga agccgatgta ggcccccagc
     8881 atgtacagcg cgccgtgggc gaagttcacc acgcgccgca ggccgtagat cagtgccagg
     8941 cccgaactca tgatgatgag aagggccccg tacaccaatc cgttgaacag attgatcgtc
     9001 aacattgcgt cgtctccttg agcccgtgga gcgggcgctg gttcttgtgt cggacggaag
     9061 gccggcgccg cagcgccggg cggggggtca gcggtggatg aacgaaggag ggcgcttttg
     9121 caagaaggcg tgcatgccct cgtgcgcgtc ggtgctggca aagcgtgcat gcagagcgcg
     9181 ccgctcgaac agaatgccct cggtcagcga gctctcccac gcgcggttca ccgactcctt
     9241 gatggccatc agggcgggca gtgagtagct tgcgatctgt ccggccagcg ccagagtgcg
     9301 cgccagcagc tcggcgtcgg gtaccacgcg tgataccagg ccgtagcggt ctgcctcttg
     9361 cgcgtccagc gtgcgggcgg acaggcacat gtccatggcc ttggccttgc cgacggcgcg
     9421 cggcaggcgc tgcgtgccgc cggcgcccgg cagcatggcc agcttgatct cgggcagcgc
     9481 gaaacgcgcc gactccgccg ctacgacgat gtcgcaggcc agcgccagct cgcatcctcc
     9541 gcccatggcg tagccggcca ctgccgcaag cacgggcttg cggatggtgc ggatggtctc
     9601 ccagttgcga gtgatgaagt cgccctgata gacatccatg tacgaccagt cggccatggc
     9661 gtcgatgtcg gcgccagcgg caaaggcctt gtcgctgcca gtgatgacga tggcgccgat
     9721 gcctgggttg gcgtcgaacg acttaagggc tgcgcccagc ttatccatca gggcatcgtt
     9781 cagggcattg agctggcgcg ggcggtgcaa ggtgatcagc cccacgcggc catgcgtggc
     9841 gacctggatc gggcgaagac tgtcgtgcgg cttgtccatg acgatcacgc ctagttgctc
     9901 ttgatcagct cttgcaccgc atcgcgcagc ttgaacttct ggatcttccc tgatggggtg
     9961 gcaggcatgg cttcctgcac gatcaggcgt tcgggcatgt actgaacggc gatctgttgc
    10021 tctttcagga agcgttggat ttccggcagg tcgatgctct gcccggcctt gggtaccacc
    10081 acggcgcagg cacgctcgcc caggcgctcg tcggggtagg cgacgatggc ggccatggcg
    10141 acggcggggt gcttgtacag cagtgactcg atttccacta cgggaatgtt ctcgccgccg
    10201 cggatgatca cgtccttgct gcgcccggtg atgcgcacat agccgcgttc gtcgatgcgc
    10261 gccaggtcgc cggtgtcgaa ccagtcgtcc gcgtcggtgc cgttgagctg tgggcgcttg
    10321 agatagccgc caaagttgga gcaggcgcgc agcagcagct tgcccgattc gccggacggc
    10381 aagctggctc cgtccgcgtc caccaccttg atctccacgc cgggcagggg acagccgtcg
    10441 gtgttgaagg agcgctcgtc gtcgtcatcc agctttgtca gggtgacggc gccgttctcc
    10501 gtcatgcccc aggccgacac gatcttcgcc cccagcgccg cacgcgcctg ctgcaccagc
    10561 gggccgggga tcggtgcgcc cgcgcacaga aaggtgcgca aagtcggcac gccggggccg
    10621 ccggcctgca cgtcctcggc cacggtgcgc gcaaggtcgg tcaggaacgg ggtggatgcc
    10681 atggtgaagg tgaccctctc cgactggatc agggagagcg cctttttcgg ctcccatatg
    10741 tcctggagca caacactggt cttgagcaga atgggcatca tcaggccgta catgaagccc
    10801 gtctggtgcg ccatagggga ggccatgagc accacgtcct cggcatccag cttcaggcgc
    10861 tcggcatagg gaatgatgtt ggccatgacc gtgttggccg aatgcatcac ccccttgggc
    10921 tcgccggtgg tgcctgaggt gtagatgagc tgtgtcacgt cgtccgggcc ggggcggccg
    10981 cgggtgagga tgtcctgcgc gtctgcctgc ttttcccatt cgggggcgct cagcaaggcg
    11041 tcgaagctgt tgcctccgtc gccaccgacg atgaccacat gtttcaggtg cggcagactg
    11101 ggctgcaagc cagtcaccat ggcctcgaag tcaaagcccc gaaaggtctt gggcgcaatc
    11161 atgaccttcg cttcgccgtg gttgagcatg aatgacagct cacgctcgcg gaagatgtgc
    11221 atcagcgggt tcatcacggc gccgatgcgc gagcaggcca ggtaggtcag cgtgaactgc
    11281 caccagttgg gcaactggca cgccaccacg tcgccccggc ccactccaag gcgggccagg
    11341 ccgacagcga tgcggtcggc catctgcgcc atttcgccgt agctgaaccg gcgcacgtcg
    11401 cccgtttcca cctgcacggc cgtcagcgcg agctgttggg ggcgggccgc cgcgcaggca
    11461 tccagctcgg cgttgatggt gcggtcatgc cacaggcctt gggcgatcag gcgcgcgcgg
    11521 cgcgggggca gcagtacggc gtcgaattcc atgggaatgt ctccaatcag gatttgctat
    11581 aaaaaatgca gctactggcg cttgctggat aagcgttatt gcgttattta ttgtttttta
    11641 tgagggattg cgcccagggc ggtgcctggg ggcaacgggg gtgtcctcag gccggcacga
    11701 atttgcggcc ggcacgcgtg cgcgccacga tggttttcat gatctgcgcc gtgccgtcgc
    11761 cgatctggaa gcccagcacg tcgcgcaggc gctgctccat cgggccgcgg tcgtagccgc
    11821 cgtggccaaa gcacagcagg cactggtgga tcacgtcgta ggccagcttg gggccccacc
    11881 acttggccat gccggcctcg gcgctgtgcg gcagaccctg gtccttgagc cacagcccct
    11941 gcaggcacaa caggcgcgcg ccttcgacct cggtgtcgta ggtcgccagc gggtgcgtca
    12001 cgccctggaa ggccgccagc ggctggccga aggcctcacg ctcggtgatg tactgccagg
    12061 cctcctccaa cgccacgcgc gccaccgcca ggcattgcag gccgatgagc gagcgtgaga
    12121 agtcgaagcc gtgcatcacc tgcacaaagc ccttgttctc gtcccccagg cggtgattga
    12181 cgggcacgcg cgcgttctcg aagaagatgg acccgcgccc gatggcgcgc tggccgtggc
    12241 agtcaaagcg gttggtggtc acgccgggcg tgtccatcgg taccagcagc gcggttacgc
    12301 catgcgcgcc ggactcgggt gtgccggtgc gcccgaacac cacgcagatg tcagcctggt
    12361 cggcggcaga gatggaggtc ttctcgccgt tgatgacgta ttcgtcgccc acgcgctcga
    12421 tcttcagccg caggttggcc gcgtccgatc cgccgcgtgg ctcggtcagc gcgatggcgc
    12481 agatgctctc gccggcgatg attttctgca gccagggcgt gaccacctcg ggctggccat
    12541 agcgcgacag gatctggctg ttcagcgaag ccagcaggtt cacgtaggaa aagctcaggt
    12601 cggcgcgtgc gatctcctcg tggatcacgc ccgcggccag gcaacccagc cccaggccgc
    12661 cgaactgctc gggcagctcg ggcacgatga agcccagctc gcccatttcg cgcatcagcg
    12721 cgcggtcgaa ggttcggctc cggtcgcgct ccagaaaacc gggcgccacg cgctcctggg
    12781 caaaacgccg cgcggtctcg gccaatgcct gcaggtcttg gttgatatag gggttgggat
    12841 tcatcgccat gttttcacac ctcgggctgc tgcttgccgc ttacttgacg tacttgcgga
    12901 actcgggctt gcgcttttcc ttgagagcgt tgacgccctc gcgcgactcg tcggtgtcgt
    12961 agtacagctt gagcgcatac atgcccaggc cggcaatgcc ggcctggtgc gccgtgtcca
    13021 tgttgaaact gcgcttggct atggccaacg ccgtggggct gcgctcgcag agctcttcgc
    13081 caatcttctg cacctcggcg tccagctggt cgtgcgggaa gcagaagttg gccaggccca
    13141 tggccacggc ctcggcgccg ctgtagcgac ggttcaggta ccagatctcg cgcgccttct
    13201 tctcgccgac cactcgggcc agaaaagccg tcccgtagcc gggatccacg gagcccatct
    13261 tcgggccgac ctgcccgaac tgggccttgt cggagcagat cgtcaggtcg caaatcgtgg
    13321 ccagcacgtt cccgccgccg atggcgaagc cctgcacgcg ggcgatcacg ggcttgggca
    13381 cgtcgcggat ggcggtgtgc agctcttcca tgggcaggcc gatggtgccg cggccgtcgt
    13441 agttgccgtc gtgcgcggac tggtcgccgc cggtgcagaa ggcacggtcg cccgcgccgg
    13501 cgagcacgat ggcgcccacg ctcttgtcgt agccggcctt gttcagcgcc ttgatgagct
    13561 cgtcgcacgt ggtgccgcga aaggcgttca tcttgtcggg gcggttgatg gtgatccagg
    13621 ccacgccgtt cttcacttcg tacagcaggt cttcaaaggt catgatgtcg attccaggtt
    13681 gggggtgtgg gaagggtcag ccgttcatgg tcaggccgcc ggagacgctg agcacctggc
    13741 cggtgatgaa gcccgcgtcg gcgctggcga agaaggcaat ggcgctggcc agatcgtctg
    13801 gctggcccag gcggcccagc ggtatggcgc gggtgaaggc ctcgagcagc ttctcggggt
    13861 tgccggcgcc ctcggccacg ccggccagca gcgccgtatc ggtggggccg gggcagacca
    13921 cgttcagggt gatgccgtgg cgcgcatgct cgcgcgccag ggccttggtc agtgccacca
    13981 ggccgccctt gcaggcggcg tacacggcct cgcccgagga acccacgcgg gcggcgtccg
    14041 acgccacgtt gacgatgcgg cctttcttgc gctcggccat gccgggcaga acggcgtggt
    14101 gcatgtgcag ggcgccggtc aggttgatgg cgatgagccg gtcccactca gcgggcacag
    14161 tcttggtgaa gggtttgaac acgtcccagc cggcgttgtt gaccagcacg gcaacgggac
    14221 cgagctgcgc ctgtgtggcg gcgacggccg catccaccga ggcgcggtcg gtgatgtcgc
    14281 aatcgaaggc ggcggcctgg ccgccggcgg ccgtgatctc ggccaccacc ttttgcgccg
    14341 cttcgaggtt gcggtcgaat accgcgacct tggcgccttc gcgcgccagg cgccggcagg
    14401 tggcgctgcc aatgccgccg ccgccgccgg tcacgatgat ggtttcgtcc tttaatcctt
    14461 gcatgtcttc aatctccggt ctgaggggtt gctatgcttg gcgggttgcg agttcaatcc
    14521 gtcaattagg taaacatgtt gcatatatta aagatcgact ttgtcgctgt caatgtgctg
    14581 agagtgggtg aaacctatgg aaaaccagta taaaaaagtg aaagaaaccc gtatggagct
    14641 ggccaacagg ctattcttca agttgtacca atgcgcaaat atgttgcata aaaccggctc
    14701 tcgcgctgtg gagtcagagg ggcttacgac gcagcagtgg gcggtgttgg gggcgctctc
    14761 gaccacggac gagggcatga gcattggggc gctggcgcgc tatctgatgg tcagtcgcca
    14821 gaatctggcg ggcttgatag ggcgcatgga gcgccagggt catgtgtgca gcgccccgga
    14881 tgggcgcgac cggcgctcac gcctggtggc gatgacgccc gccggccgcc tcacctggga
    14941 gcgaggggcg caggccaaga tcgaggacta ctacacgcaa gcgctggagg gcttctcgac
    15001 cagcgaccag gtgcatgcgc tgcactacct gctcaaactg ctggacaaca tgcagcggat
    15061 cgatatcgcg cagggtgcga gtgaggagga tgatgcgggc tgactgccgg acctgccccg
    15121 gcagcaccgg cgcagtcgtc aggccgcccc gcccgagccg cgcgcgcccg aggacggcac
    15181 cggcagaccc agcatggcgc aggccacggc ctcgccgacc ttgatgccgt ccacgccggc
    15241 cgagagaatg ccgccggcgt agcccgcgcc ctcgccggcg gggtacaggc cctctgtgtt
    15301 caggctctgg aagtcctcgc cccggtctat gcgcagcggg gccgaggtgc gtgtctccac
    15361 gccggtcagc accgcgtcct gcatgtcgta gccacgtatc ttgcgcgcaa acgccggcag
    15421 ggcctcgcgc atcgccgcgg tggcgtaggc gggcagggcc tcgtgcagat cgaccagcgt
    15481 cacgccgggc tggtaggagg gctgcacctc gcccagcgcg gtggatgcgc gccgcgccag
    15541 aaagtcgccc acgcgctggg ccggggcgtt gtagttgccg ccgccgagct cgaaggcgcg
    15601 cgcttccagc tggcgctgca gcacgatgcc ggccagcggg tgcgtcttat ccgcggccag
    15661 tgcctccacg ccgtgcgtcg cgcccagcat ggcctcgaag gcggccgggt cgcgcggata
    15721 gtcatgcggc tccacgccca ccaccatgcc ggcattggcg ttgcgctcgg cgcgcgaata
    15781 ctggctcatg ccgttggtga ccacacgccc cggctcgctg gtggcggcga ccacggtgcc
    15841 gccggggcac atgcagaagc tgtagacggt gcggcccttg gcctggccgt tttgcgcgtg
    15901 gtgcaccagc ttgtagtcgg ccgcgcccag cagcgggtgg cccgcgtcct
//
LOCUS       DAOMTQ010000060        15888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_567_length_15888_cov_26.167625, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000060 DAOMTQ010000000
VERSION     DAOMTQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15888
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_15888_cov_26.167625"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK518_07740"
     CDS             complement(<1..427)
                     /locus_tag="PK518_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRM48844.1"
                     /translation="MSLSTITALSPLDGRYAAKLSALRPIMSEHGYMHRRVQVEVAWF
                     IALSDAGFDEFKPLSPGARAYLQGLVHNFSEADATAIKEIEKTTNHDVKAVEYWIKSK
                     FGGRPELEKAAEFVHFACTSEDINNTSHALQIRAGRDQVI"
     gene            533..1147
                     /locus_tag="PK518_07745"
     CDS             533..1147
                     /locus_tag="PK518_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRM48845.1"
                     /translation="MKLIGSTASPYVRKVRVVMAEKKLDYRFQEENVWSEETAISTAN
                     PLGKVPCLVMEGGEAVFDSRVIVEYLDTLSPVGKLIPGTGRERAEVKTWEALADGVLD
                     AGVLARLEATWAGRQDGERSQAWMARQLGKVRAGLQAMSQGLGDKPYCSGIHLSLSDI
                     AVGCALGWLEFRFPQIAWRTDHPNLARLMDKLMQRQSFIDTKPH"
     gene            complement(1597..4698)
                     /locus_tag="PK518_07750"
     CDS             complement(1597..4698)
                     /locus_tag="PK518_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM48846.1"
                     /translation="MNLSAWSIKNPIPAAMLFVLLTLAGLLSFSAMKVQNFPDMDLPV
                     VMVTAALPGAAPGQLESDVARKIENAIATTQRLKHITTTLVDGTATIAAEYQLEKPVQ
                     EAVDDVRSAVSRVRADLPADLRDPIVTKLELSSQPILAFAIASNKLDDQELSWFVDDQ
                     LTRRLLAVPGVGAVSRVGGVTREVRVALDPLKLQALGVTAAEVSHQLRQVQLESAGGR
                     ADLGGAEQPLRTLATLQTAEQIAALQIPLSGGRHARLDQLAEVSDTWAEPRTAALLGE
                     QPVIGFEVSRSRGASEIEVGAGVQQALDALRAEHPHLTLKRTVDFVEIAQDEYDSSMH
                     LLYEGAILAVVVVWLFLRNWRATIVSAVALPLSVIPAFIGMHLLGFSINVITLLALSL
                     VVGILVDDAIVEVENIVRHLRMGKTPYQAAMEAADEIGLAVIATTFTLIAVFLPTAFM
                     SGIAGRFFKQFGWTAALAVFASLVVARLLTPMMSAYLLKPLVASEHEPRWLAAYMRAS
                     HWCLRHRWLTLIGAALFFAGSLALIPLLPSGFIPADDNAQTQVMLELPPGAKLQETQA
                     LALRAGALAMGVGDVRSIYTTIGGGSAGTDPFAGAGTGDPRKATLTLQLAPRQERPRK
                     QVIEQRLRQAMAELPGARVKVGLGGSNDKYILALASEDPQALAEAARAVERDIRTIGG
                     IGGVSSTASLVRPEIAVRPDFARAADLGVTSQAIAETLRVATVGDYEQFLPKLNLAQR
                     QVPIVVRLDDAARQDLSVLERLAVPGNRGPVRLGEVASLTLAGGPAVINRYDRSRNVN
                     FEIELGSRGLGEVTEAVRQLPAVQGLPASVRLIDVGDAEMMGELFASFGLAMLTGVVC
                     IYIVLVLLFKDFLQPVTILAALPLSLGGAFVALLVAGQSFSMPSLIGLIMLMGIATKN
                     SILLVEYAIVARRAGMERLHALLDACHKRARPIIMTTLAMGAGMMPIALSLGGADMSF
                     RSPMAVAVIGGLITSTVLSLLVVPAVFTYVDDFEQWLRRHWPHREARMAPNGNKVQPP
                     SLP"
     gene            complement(4729..5821)
                     /locus_tag="PK518_07755"
                     /pseudo
     CDS             complement(4729..5821)
                     /locus_tag="PK518_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(5840..8584)
                     /gene="glnE"
                     /locus_tag="PK518_07760"
     CDS             complement(5840..8584)
                     /gene="glnE"
                     /locus_tag="PK518_07760"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HRM48847.1"
                     /translation="MQMPSTDTAASSEPLSHHSRFQQRLQRRYGAELTLLAPGRPDHA
                     RMNQACDALLARGHDLGAALRILRQLVMHRLITLDCDQGAPLADITGTVTALAELALD
                     RACRAARAELDARHGVPLGPQGQPVQLWVIGMGKLGARELNVSSDIDLIYVYEQDGDT
                     AGLPDGRGRISNHEYFARAVKAIYALVGDTTEHGFVFRVDLALRPHGNSGPAALSLSA
                     LEDYLQVHGREWERFAWLKSRIVAPLDCIASPQVQALRAVVLPFVFRRYLDYNVFDAL
                     RALHHQIRNHAVQRSAGRPERANDVKLSRGGIREIEFTVQLLQVVRGGQFPELRRRPT
                     LEALERLARAGLMPEPTARQLAEAYVFLRRVEHRIQYLDDQQTHVLPTRDEDLAWIGR
                     TMGLGCCDFLRELDTHRELVAQEFDTLLGGGEHKRCANGACNKAQAQAQAHAAPAPDL
                     DALLEQLPPALSARVAEWRNHQRVQGLRDEARARLFRIVQRTARWLADGRASEDAAVR
                     LVGWLEPLLRRESYLAMLLERPLVHERLLQLLGAAKWPARYMLQHPGVIDELASEAML
                     AERFDAADFEHELALRLQALRSTGEDDDENLLNLLRRAHHAEVFRTLARDVDGRLTVE
                     QVADDLSLLADSVLRVTAQWCWSRLKNRHREAPQFAVLGYGKLGGKELGYGSDLDIVF
                     VFDDEDERAPEVYAAFVRKLINWLSAKTGEGDLFEIDTALRPNGNSGLLVTSFQAYAD
                     YQQQRGSNTAWTWEHQAMTRARFVLGSEDLRARFDAVREAVISAPRDNAALSGEITAM
                     RQRMRAANPVPAGQFEVKHSPGGMLDVEFAVQFLVLAHSGAHPELLENKGNIALLARA
                     EDAGLLPAGVGRAAADAYRTLRQEQHRARLNEEPTRVPDSELAAEQGAVLALWHAVFG
                     "
     gene            8658..12734
                     /locus_tag="PK518_07765"
     CDS             8658..12734
                     /locus_tag="PK518_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HRM48848.1"
                     /translation="MTEPSSHPSRLLRLVAGCARWGLGLLLALWLLLAAAWGGLHGWI
                     VPRIGDYRPLLEAQAGRALGIPVRIAAISARSEGLIPTVELAGVTLLDAQGRDALQLP
                     RVVLALSPRSLLRLGFEQIYIEGPALDVRRMADGRITVAGLALQRDEQGESGAADWLF
                     DQPEVAIRGGTLRWSDEQTGAEPLALTQVDLVLRNGGWRHAMRLDATPPPGWGERFSL
                     RGRFRQPLLTTHGGNWRRWSGQLYADFARVDVSRLNQYADLGALEVAQGHGALRAWLD
                     WQRGQLAGATVDMALGEVNATLGQDLRPLVLRTVTGRLGGRWLEGGFELSSDNLQFQA
                     DDGLAWPGGKLFLRHTVADRAGREQGELQADRLDLAALAQIASRLPLDAAVRDALQTY
                     APQGLVQQLQAGWHGPLAQPRQYQLRAKVSGLAIAAQARNPAVVGLTGADVELDLTQQ
                     GGRAELAMADGRLLLPGVFEDPVLPVQQLSATLRWQVEGQRIAVQTNDLRFANADAQG
                     EARLAWRTADAATSPARSRFPGVLELSGHLSRADGTRVHRYLPLVIPAETRHYVRDAI
                     QTGSAPEVQFRVKGDLHDFPYGHAPRLGEFHIAARVTDVTYAYVPPSLLAPGDRPWPV
                     LTNLAGELVFDRASMLVRNATGGFAGQRALRVQKVGAKIADLEHSVVEVDGQVRGPLQ
                     DMLTLVSGSQIAQLTHGALDRATASGAADLQLALKLPLAKMAQSQVRGSVTLADNDVR
                     FVPEAPTVSGARGVVQFSDTGFSLVGVQGRALGGELRLEGGMVAPPAAGARESPVRVR
                     ARGTATAEGLRETPQLGLLARLARHASGASAYALSLGVRRGVAEIQVTSDLVGIALDA
                     PAPLGKAAATPLAMRFDNQLTSEAMASDSAPLQDQLTLALGDVLRLDYLRALEGEQAR
                     VLRGAVTVGLPAGQAMALPQQGVAANARLDMLDVDAWDALLDQHQEGPGAGGPVAQAQ
                     GYLPDRVALRVGTLALQGRSLHDVVAGVSRAGADWRANVSARELDGYVEYRQADGAAQ
                     QEGLYARLSRLALPQSDEPQVDALLDAQQPARLPALDIVVQDFELRGRRLGRLEIEAR
                     NRATQDGQREWRLGKFNLAAPEAVFASSGSWALVGGKGAAAQRRTSMDFALDIRDSGA
                     LLARFGMDGVLRRGKGRIAGQVGWQGSPLSPDYRSMTGQMHVDVESGQFLKADPGLAK
                     LLGVLSLQSLPRRLVLDFRDVFSEGFAFDFVRGDLHVEQGVATTNNLQMKGVNAAVLM
                     EGSADIERETQDLHVVVVPEINAMTASLVATAINPVIGLGSFLAQVFLRGPLIQAATQ
                     EFRIDGTWAEPRVERIQQRQAAPGAGGPGAPATSGAKP"
     gene            12731..13546
                     /locus_tag="PK518_07770"
     CDS             12731..13546
                     /locus_tag="PK518_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRM48849.1"
                     /translation="MKVAALQMVSGVALQPNLEVARQLLQQAARAGAELAVLPEYFCA
                     MGRRDEDKLALRERLGDGPVQRFLADAARELGLWIVGGTLPLAGDDPRRVYNSSLAFS
                     PAGECVARYDKIHLFRFDNGREQFDEARTIQAGQGSVRFALPARDGRLWHVGLSVCYD
                     LRFPELYREHARAGADLLLVPSAFTYTTGRAHWELLLRARAVENLAYVLAPAQGGQHE
                     NGRRTWGHSMCVDPWGQVLARQAEGSGVVLGELDAAHLRQLRSQLPALEHRVL"
     gene            13546..15615
                     /locus_tag="PK518_07775"
     CDS             13546..15615
                     /locus_tag="PK518_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRM48850.1"
                     /translation="MTETAAPHEPAAPPLERLPGRWRRWLGAWRRWSLWALLLALVAS
                     MLVTLVWLAGRYEAGEVQSRLERDSADAVADIRNAFAHNLQNLQALQAGDPDLAIWES
                     RAGDLLTQRRELVRIEWRDTQLQLRAHAQTPYRPQGRDPTQRASVRSETALACANARR
                     ITGPSYSTSYFQPMPDGVGAEMMEVCLPLMGDGRNHGFLVATYALHTVLQELVAPGLK
                     RTQEVSFTEADGTRLALVGAMRRGTRMFTAQQLFDLPGNTLILRMDGWHAAPSVFPNV
                     LTALVTAMSIALVSVLVVLVRDNRRRLSAERDLGDALAFRKAMEDSLVTGLRARDIHG
                     RITYVNPAFCAMVGFSTSELVGLDTPPYWPPEHAREYAERQMVRMAGRLDPPREGYES
                     TFMRKDGTRLTVMIFEAPLINAQGLHTGWMGSFLDVTEQRRAEELSRTSQERLQATAR
                     LATVGEMASLLSHELNQPLAAIASYASGSLNLLGPAQAAQPPSAHDLADLRVGVQRIA
                     AQAERAGRVIKSVHDFVRRRDQSRAPASAQALFDAVLPLIRLQAHKIGVRVVLDIQAG
                     LPPVLCDRTMVEQVLLNLARNAMQSMDDPQIHPRILDLHVRRAASNAARGWLEFSVAD
                     MGSGIPEDVAERLFTPFYTTRAEGMGLGLSLCRTVVEQHGGFLGHAQRQPRGTVFTFT
                     LPVFQED"
     gene            15615..>15888
                     /locus_tag="PK518_07780"
     CDS             15615..>15888
                     /locus_tag="PK518_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM48851.1"
                     /translation="MEPAANATVYIVDDDAEVREALAWLLRSRRVLSEAYASAEAFEL
                     MLAERATAGLPQRQPCCLLLDVRMPGMSGLALFDNMVARGDTAGIPV"
BASE COUNT         2326 a         5363 c         5759 g         2440 t
ORIGIN      
        1 caatgacctg gtcgcgcccg gcgcggatct gcagcgcgtg gctggtgttg ttgatgtctt
       61 cgctggtgca ggcgaaatgc acgaactcgg cggccttctc cagctccggg cggccaccga
      121 acttggactt gatccagtac tccaccgcct tcacgtcgtg attggtggtc ttttctatct
      181 ccttgatggc ggtggcatcg gcctcggaga agttgtgcac caggccttgc aggtaggcgc
      241 gggcgcccgg gctcagcggc ttgaactcgt caaaccccgc gtccgacagc gcgatgaacc
      301 aggcgacctc cacctgcaca cggcggtgca tgtaaccatg ctcgctcatg atggggcgca
      361 gggcggagag cttggcggcg tagcggccgt caagcggcga cagggcggta atcgtggaca
      421 gactcatgat ggctgtgatt gtaggtgcgc acctgacacc gcgttgaccg tggggcgcct
      481 atagaatggg ccgccctgac accagccgcc cgtccgcggc cgaacaacca tcatgaagct
      541 gatcggatcc accgctagcc cctatgtgcg caaggtgcgc gtggtgatgg cagagaagaa
      601 gctggactac cgttttcagg aagaaaacgt gtggtccgag gaaaccgcca tcagcaccgc
      661 caaccccctg ggcaaggtgc cctgcctggt catggaggga ggcgaggcgg tgttcgattc
      721 acgcgtcatc gtggaatacc tggacacgct ctcgcccgtg ggcaagctca ttcccggcac
      781 ggggcgcgag cgtgccgagg tcaagacctg ggaggccctg gccgatggcg tgctggacgc
      841 cggggtgctg gcacggctgg aggccacctg ggccggacgc caggacggcg agcgcagcca
      901 ggcctggatg gcgcgccagc tgggcaaggt gcgcgccggc ctgcaggcca tgagccaggg
      961 cctgggcgac aagccgtatt gcagcggcat acacctgagc ctgtcggata ttgcggtcgg
     1021 ctgcgccctg ggctggctgg agttccgctt tccgcagatc gcctggcgca ccgatcaccc
     1081 caacctggcg cggctgatgg acaagctgat gcagcgccag agcttcatcg acaccaagcc
     1141 gcactgacca gcaccgaccc caagaaaaaa gcgcgccttc cgaagaggaa gtgcgcgctt
     1201 attgctgcaa aaaaattgtt gcgaagcgcc ccgaaacgaa tgaagagagg gagggaggag
     1261 aagcgcccca ggattgcatc caggcacggc ctggaaggct tcgcaacagt aaaaaagatt
     1321 caatgatcga tgggcctgtc aaccacctgt gccagtgaga gtgagggcaa aggccgaaag
     1381 ttccagtctt tttaacaaaa aatttaaaac tgtggtgccg ccgaaacgct gcaccgcgtg
     1441 cggagttctc gcagaactgc acgccggtgg tgttactgtc tgtgtcttgt gcaatggcgt
     1501 gggccagggt tccagagacc tgcgccatgc tccgtggcag catgtgcgcc gctgtgtgaa
     1561 tgaggcatgc gcttcggcgc agccccttgg gcagcgtcaa ggcagcgatg gcggttgcac
     1621 cttgttgccg tttggtgcca tgcgcgcctc ccgatgtggc cagtgccggc gcagccattg
     1681 ctcgaaatca tcgacgtagg tgaagaccgc gggcacgacc agcaggctca gcacggtgga
     1741 ggtgatgagc ccgccgatca cggccacggc catgggcgag cgaaagctca tgtccgcccc
     1801 gcccagggac agcgcgatgg gcatcatgcc cgcgcccatg gccagcgtgg tcatgatgat
     1861 gggacgggcg cgcttgtggc aggcgtccag cagcgcatgc agccgctcca tgccggcgcg
     1921 gcgcgcgacg atggcgtatt ccaccagcag gatggagttc ttggtggcga tgcccatgag
     1981 catgatgagc ccgatgaggg acggcatgga aaagctctgg ccggcgacga gcagcgccac
     2041 gaaggccccg cccagcgaca gtggcagggc cgcgaggatg gtcacgggct gcaggaagtc
     2101 cttgaacagc agcaccagca cgatgtagat gcagaccacg ccggtgagca tggccaggcc
     2161 gaagctggcg aacagctcgc ccatcatctc cgcgtcgccc acgtcgatca ggcgcacgct
     2221 ggccggcagg ccctgcacgg cgggcagctg gcgcacggct tcggtcacct cacccaggcc
     2281 acggctgccc agctcgatct cgaagttcac gttgcgcgag cggtcgtagc ggttgatcac
     2341 cgccgggccg ccggccagcg tcaggctggc cacctcgccc aggcgcaccg ggccgcggtt
     2401 gcccggcacg gccaggcgct cgagcaccga cagatcctgg cgcgccgcgt catcgagacg
     2461 tacgacgatg ggcacctggc gctgcgccag attcagcttg ggcagaaact gctcgtagtc
     2521 gcccacggtg gccacgcgca gggtctcggc gatggcttgc gaggtcacgc ccaggtcggc
     2581 ggcgcgcgca aagtcgggcc gcacggcgat ctcgggccgc accaggctgg ccgtgctgct
     2641 gacgccgccg atgccgccta tggtgcggat gtcacgctcc acggcgcgcg ccgcctcggc
     2701 cagggcctgc gggtcttcgc tggccagggc caggatgtat ttgtcgttgg agccgcccag
     2761 gcccaccttg acgcgcgcgc cgggcagctc ggccatggcc tggcgcaggc gttgctctat
     2821 gacctgtttg cgcgggcgct cctggcgtgg tgccagttgc agggtgagtg tggccttgcg
     2881 cgggtcgccc gtgccggcgc cggcaaatgg gtcggtgccg gccgagccgc cgcctatggt
     2941 ggtgtagatg ctgcgcacat cgcccacgcc catggccagg gccccggcgc gcagggccag
     3001 ggcctgggtc tcctgcagct tggctccggg gggcagctcc agcatcacct gggtctgggc
     3061 gttgtcgtcg gccggtatga agcctgaggg cagcagcggg atcagcgcca gcgagccggc
     3121 gaagaacagg gctgcgccga tcagggtcag ccagcgatgg cgcaggcacc agtggctggc
     3181 acgcatgtag gcggccagcc agcgcggctc gtgttcggac gccaccaggg gcttgagcag
     3241 gtaggccgac atcatgggcg tgagcaaccg cgccaccacc agagaggcga acaccgccag
     3301 cgccgccgtc cagccgaact gtttgaagaa gcgcccggca atgccgctca tgaaggccgt
     3361 gggcaggaac acggcgatca gcgtgaaggt ggtggcgatg acggccaggc cgatctcgtc
     3421 ggccgcctcc atggccgcct ggtagggcgt cttgcccatg cgcaggtggc gcacgatgtt
     3481 ttccacctcg acgatggcgt cgtccaccag gatgccgacc accagcgaca gcgccagcag
     3541 ggtgatgacg ttgatggaaa aacccagcag atgcatgccg atgaaggccg gtatcaccga
     3601 caacggcagg gccacggccg agactatggt ggcccgccag ttgcgcaaaa acagccagac
     3661 caccaccacg gcgaggatgg cgccctcgta cagcaggtgc atggagctgt cgtactcgtc
     3721 ctgcgcgatc tcgacaaagt ccacggtgcg cttgagcgtc agatgcgggt gctcggcgcg
     3781 cagggcatcg agcgcctgct gcacgcccgc gcccacctcg atctcgctgg cgccgcgact
     3841 gcgcgagacc tcgaagccga tcaccggctg ctcgcccagc agcgcggccg tgcgcggctc
     3901 ggcccaggtg tcgctgacct cggccagctg atccaggcgc gcatggcgcc cgcccgagag
     3961 cgggatctgc agcgccgcga tctgctcggc cgtctgcagc gtggccaggg tgcgcagcgg
     4021 ctgttcggcg ccgcctaagt cggcgcgccc gcctgcgctc tccagctgca cctggcgcag
     4081 ctggtgcgag acctcggccg ccgtcacccc cagggcctgc agcttgagtg gatcgagcgc
     4141 cacgcgcacc tcgcgcgtca cgccgcccac gcggctcacg gcgcccacgc ccggcacggc
     4201 cagcaggcgg cgcgtgagct ggtcatcgac gaaccaggac agctcctggt cgtccagctt
     4261 gttcgaggca atggcgaagg ccaggatggg ctgggaggac agctccagct tggtgactat
     4321 ggggtcgcgc agatccgccg gcaggtcggc gcgcacgcgc gagacggccg agcgcacatc
     4381 gtccacggcc tcctgcacgg gtttttccag ctggtactcg gcggcgatgg tggccgtgcc
     4441 atcgaccagc gtggtggtga tgtgcttgag ccgctgcgtg gtggcgatgg cgttctcgat
     4501 cttgcgcgcc acgtccgatt caagctgccc cggcgccgcg cccggcaacg ccgccgtgac
     4561 catgaccacg ggcaggtcca tgtcgggaaa gttctgcacc ttcatggcgg agaacgacag
     4621 cagcccggcc agcgtgagca gcacgaacag catggccgcc ggtatggggt tcttgattga
     4681 ccaggccgac agattcatga ttacttctta tttgatagct atttgcgctt atttgacggg
     4741 cgctgcaggc tgtttttgtg ctgaatcgct gggggccacg cgcaccaggt cgccatcatt
     4801 cagaaaggcg ccgccctgct gcacgacgcg cgcctcgggc gtcaggccgg tggtgatctc
     4861 cacgcggtcg gcgttgcgct ggccggtctg cacgcggcgc atcagcacgc ggccgtcggc
     4921 tcccacctcg aatacctggc tgaagccgtc gcgcaccacc accgcggcca gcggcacggt
     4981 cagggcttcg tgctggccca gaacgaactc tccgcgcgcg aacatgccgg cgcggatatc
     5041 ggggtgctcg ggcagatcca catacaccag cgcgttgcgc gtggccgcat ccaccgtggg
     5101 agcgagcacg cgcacccggc cctcgacctg ggcgccactg gcggccgtga ccatggcccg
     5161 gccgccggcg cgcagcagcg gcaggtcgct ggaggtgacc tcggcgcgcc actccaggcg
     5221 gccgcggcgc agcatgcgaa acagctccgt gcccgggccc gccaccgcgc ccacggtggc
     5281 caggcgcgcg gagatcaccc cggcatcggg cgccagcacg cgcctccagt tgcgcctggc
     5341 tggccgccac gcgcgcctgg gcggtctgcg cggcggtgct gtattgcagg atctcctgct
     5401 ggctcatggc gccggtggcg ctcagtgcgc gggcccgctc ggcgttggcc tgggcctggg
     5461 ccagtgcggc gcgtgcctcc tgcaggccgg cacgggcctg ggccaggtcg gcgcgcacgc
     5521 tgtcgtcggc aaagcgcgcc agcagttggc cggcgcgcac ctgctcaccc acgttcacgc
     5581 gcacctcggc caggcgcagg ccgctgcttt cgctgcctat gctggcctcc tgccaggcgg
     5641 ccacgctgcc attggccggc aggcgccgcg ccagcgactc gcgccttggc tgggtggtgc
     5701 tgacggtgag ggccgggcgc ggggcgctgg ccgtggtggt ggcggcagca gcggtgggca
     5761 aggggctggc ggcggccagc aggccgagcc cggccagggc gatcaggagg gaatgtttca
     5821 tggaaggagg gtgatgtctt cagccaaaca cggcgtgcca cagggccagc acggcgcctt
     5881 gctcggcggc cagctcgctg tcgggtacgc gcgtgggttc ttcgttcagg cgcgcgcggt
     5941 gctgctcctg gcgcagcgtg cggtaggcgt ctgccgccgc gcggcccacg ccggcgggca
     6001 gcaggccggc gtcttcggcg cgcgccagca gggcgatgtt gcccttgttc tccagcagtt
     6061 cggggtgggc gccgctgtgc gccagcacca ggaattgcac ggcaaattcc acgtccagca
     6121 tgccgcctgg gctgtgcttg acctcgaact gaccggcggg caccgggttg gccgcgcgca
     6181 tgcgctggcg catggcggtg atctcgccgg acagggcagc gttgtcgcgt ggcgcgctga
     6241 tgacggcctc gcgcacggca tcaaagcgtg cgcgcaggtc ttcgctgccg agcacgaagc
     6301 gtgcgcgcgt catggcctgg tgctcccagg tccaggcggt gttgctgccg cgctgctgct
     6361 ggtagtcggc ataggcctgg aagctggtca ccagcaggcc cgagttgccg ttggggcgca
     6421 gggcggtgtc gatctcgaac aggtcgccct cgccggtctt ggccgagagc cagttgatga
     6481 gcttgcgcac gaaggctgcg tagacctcgg gggcgcgctc atcctcgtcg tcgaagacga
     6541 agacgatgtc caggtcactg ccatagccca gttccttgcc gccgagcttg ccgtagccca
     6601 gcacggcgaa ctgcggcgcc tcgcggtggc ggttcttcag ccggctccag caccactggg
     6661 cggtgacgcg cagcacgctg tcggccagca gcgacaggtc gtcggccacc tgctccacgg
     6721 tgaggcgccc gtccacgtcg cgcgccaggg tgcgaaacac ctcggcatgg tgggcgcggc
     6781 gcagcaggtt gagcaggttt tcgtcgtcgt cctcgccggt cgatcgcagg gcctgcaggc
     6841 gcagggccag ctcatgctcg aagtcggcgg cgtcgaagcg ctcggccagc atggcctcgc
     6901 tggccagctc gtcgatcacg cccgggtgct gcagcatgta gcgcgccggc catttggccg
     6961 cgcccagcag ctgcagcagg cgctcgtgca ccagggggcg ctccagcagc atggccaggt
     7021 agctctcacg gcgcagcagg ggttccagcc agcccaccag gcgcacggcg gcgtcttcgc
     7081 tggcgcgccc gtcggccagc cagcgtgcgg tgcgctgcac gatgcgaaac aggcgcgcgc
     7141 gcgcctcgtc gcgcaggccc tgcacgcgct ggtggttgcg ccattcggcc acgcgcgcgc
     7201 tcagggcggg cggcagttgc tccagcaggg cgtccaggtc gggtgccggc gcggcatggg
     7261 cctgcgcctg cgcttgcgcc ttgttgcagg caccattggc gcagcgcttg tgctcgccgc
     7321 cgccgagcag ggtgtcgaat tcctgcgcca cgagctcgcg gtgggtgtcc agttcgcgca
     7381 gaaaatcgca acaacccagg cccatggtgc ggccgatcca ggccaggtct tcatcgcgcg
     7441 tgggcagcac atgggtctgc tggtcgtcca ggtactggat gcggtgctcc acgcggcgca
     7501 gaaagacata ggcctcggcc agctgccggg ccgtcggttc gggcatcagg ccggcgcgcg
     7561 ccaggcgttc aagcgcctcc agcgtgggcc ggcggcgcaa ctcgggaaac tggccgccgc
     7621 gcaccacctg caacagttgc acggtgaatt cgatctcgcg gatgccgccg cgcgagagct
     7681 tgacgtcgtt ggcgcgctcg ggccggccgg cgctgcgttg caccgcgtgg ttgcggatct
     7741 ggtggtgcag cgcgcgcagc gcgtcgaaga cgttgtagtc caagtagcgg cgaaacacaa
     7801 agggcagcac caccgcgcgc agcgcctgca cctgcgggct ggcaatgcag tccagcggcg
     7861 cgacgatgcg gctcttgagc caggcaaagc gctcccactc gcggccgtgc acctgcaggt
     7921 agtcctccag cgccgataac gacagggccg ccggccccga attgccatgc gggcgcagtg
     7981 ccagatccac gcggaacacg aagccatgct cggtggtgtc gcccaccagg gcgtagatgg
     8041 ccttcaccgc gcgcgcaaaa tattcgtggt tggagatccg gccgcgccca tcgggcaggc
     8101 cggcggtgtc gccgtcctgc tcgtagacgt agatcaggtc gatgtcgctg gatacgttga
     8161 gctcgcgcgc gcccagcttg cccatgccaa tcacccacag ctgcaccggc tggccctggg
     8221 ggcccagcgg cacgccgtgg cgcgcgtcca gctcggcacg cgcggcgcgg caggcgcgat
     8281 cgagcgccag ttcggccagc gcggtcacgg tgccggtgat gtcggccagt ggtgcaccct
     8341 ggtcgcagtc cagcgtgatg agccggtgca tgaccaactg gcgcaatatg cgcagcgccg
     8401 cgcccaggtc atgaccgcgc gccagcaggg catcgcaggc ctggttcatc ctggcatggt
     8461 cgggccggcc cggcgccagc aaggtcagct cggcgccgta acggcgctgc aggcgctgct
     8521 gaaagcgtga atggtgcgag agtggctcgc tggaggctgc ggtgtcggtc gaaggcatct
     8581 gcatggcaaa ggcgggtcgg ctgctgaacc ctcagggggt gggcgggtca taattcctgc
     8641 caccgttttc agccgtcatg acagagccat cgtcccatcc ttcccgcctg ctcagattgg
     8701 tggcgggctg tgcgcggtgg gggctggggc tgttgctggc cctgtggctg ttgctggcgg
     8761 cggcctgggg ggggctgcat ggctggattg tgccgcgtat cggcgactac cgccccctgc
     8821 tggaggcaca agccgggcgc gccctgggga ttcccgtgcg cattgccgcg atctcggcgc
     8881 gcagcgaggg gctgatcccc accgtggagt tggccggcgt gaccctgctg gacgcgcagg
     8941 ggcgcgatgc cctgcagctg ccgcgcgtgg tgctggcgct gtcgccgcgc tcgctgctgc
     9001 gcctgggctt cgagcagatc tatatcgaag gcccggcgct ggacgtgcgc cgcatggccg
     9061 atggccgcat caccgttgcc ggactggctc tgcagcgcga tgagcagggc gaaagcggcg
     9121 ccgccgattg gctgtttgac cagcccgagg tcgccatccg gggcggcacg ctgcgctgga
     9181 gtgatgaaca aaccggggcc gagccgctgg cgctgaccca ggtggatctg gtgctgcgca
     9241 atggcggctg gcgccatgcc atgcgcctgg atgccacgcc gccgccgggc tggggggagc
     9301 gcttttcgct gcgcggacgg tttcgccagc cgctgctgac cacccatggt ggcaactggc
     9361 ggcgctggag cggccagctt tacgccgact ttgcgcgcgt ggatgtctcg cgcctgaacc
     9421 agtatgccga tctgggcgcg ctggaggtgg cccagggtca tggcgcgttg cgtgcctggc
     9481 tggactggca gcgtggtcag ctggccgggg ccacggtgga catggcgctc ggcgaggtga
     9541 acgccacgct gggccaggat ctgcgcccgc tggtgctgcg cacggtcacg gggcgcctgg
     9601 gcgggcgttg gctggagggg ggcttcgagc tcagtagcga caacctgcaa ttccaggccg
     9661 acgatggcct cgcctggcct ggcggcaagc tgttcctgcg ccataccgtg gccgaccgtg
     9721 cggggcgtga gcagggcgag ctgcaggccg atcgcctcga tctggccgcc ctggcgcaga
     9781 ttgccagccg tctgccgctg gatgccgccg tgcgtgacgc cctgcagacc tatgcacccc
     9841 agggtctggt gcagcagctg caggccggct ggcatgggcc gctggcgcag ccccggcaat
     9901 accagctgcg cgccaaggtg agcggcctgg ccattgccgc ccaggccagg aatcccgcgg
     9961 tcgtcggcct gactggcgcc gatgtagagc tggatctgac gcaacagggc ggacgcgcgg
    10021 agctggccat ggcagacgga cggctgctgc tgcccggtgt gttcgaagac ccggtgctgc
    10081 ccgtgcagca gctgtcggcc acgctgcgct ggcaggtcga ggggcagcgc atcgcggtgc
    10141 agaccaacga cctgcgcttt gccaatgccg acgcccaggg cgaggcgcgc ctggcctggc
    10201 gcacggcgga cgccgccaca tcaccggcac gctcgcgctt tccgggcgtg ctggagctct
    10261 cgggccacct gtcgcgcgcc gacggcacgc gcgtgcaccg ctacctgccg ctggtcatcc
    10321 cggccgagac gcgccactac gtgcgcgacg ccatccagac gggcagtgcc cccgaggtgc
    10381 agttccgcgt caagggcgat ctgcacgact ttccctatgg ccatgcgccg cgcctgggcg
    10441 aatttcacat cgccgcgcgc gtcacggatg tgacctacgc ctacgtgccg cccagcctgc
    10501 tggcgcccgg cgaccggccc tggccggtgc tgaccaacct ggccggcgag ctggtgtttg
    10561 accgcgccag catgctggtg cgcaatgcca cgggcggttt tgccggccag cgggcgctgc
    10621 gcgtgcagaa ggttggggca aagatcgccg atctggagca cagcgtggtc gaggtcgatg
    10681 gccaggtgcg cggcccgctg caggacatgc tgacgctggt gagcggctcg cagattgcgc
    10741 agctcaccca tggcgcgctc gatcgcgcca cggccagtgg cgcggccgat ctgcagctcg
    10801 ccctgaagct gccgctggcg aagatggccc agtcccaggt gcgcggcagc gtcaccctgg
    10861 ccgacaacga tgtgcgcttt gtgcccgagg cgccgacggt gagcggcgcg cgcggcgtgg
    10921 tgcagttcag cgacaccggc ttcagcctgg tcggcgtgca gggccgggcc ctgggcggcg
    10981 agctgcgact ggagggcggc atggtggcgc cgcccgcggc cggcgcgcgc gaatcaccgg
    11041 tgcgcgtgcg tgcccgcggc acggccacgg ccgagggcct gcgcgagacg ccccaactgg
    11101 gcctgctggc gcgactggcg cggcacgcca gcggtgccag cgcctatgcc ttgagcctgg
    11161 gcgtgcgccg cggcgtggcc gagatccagg tcaccagtga cctggtgggc atagccctgg
    11221 acgccccggc gccgctgggc aaggccgcgg ccacgccgct ggccatgcgc tttgacaacc
    11281 agctcacgtc cgaggcgatg gcctccgaca gcgcgccgct gcaggatcag ctgacgctcg
    11341 ctctgggcga tgtgctgcgc ctggactacc tgcgcgcgct cgagggcgag caggcccgcg
    11401 tgctgcgcgg cgcggtgact gtcggcctgc cggcgggcca ggccatggcg ctgccgcagc
    11461 aaggcgtggc tgccaatgcc cggctggaca tgctcgatgt cgatgcctgg gacgcgctgc
    11521 tcgatcagca ccaggagggg ccgggcgcag gcggaccggt ggcccaggcc caggggtacc
    11581 tgcccgaccg ggtggcgctg cgcgtgggta cgcttgccct gcaggggcgc agcctgcacg
    11641 atgtggtggc cggcgtctcc cgcgccggcg ccgactggcg cgccaacgtc agcgcgcgtg
    11701 aactcgacgg ctatgtggag taccgccagg cggacggcgc ggcccagcag gaggggctgt
    11761 atgcgcgcct gtcgcgcctg gccctgcccc agtcggacga gccgcaggtg gatgcgctgc
    11821 tggatgcgca gcagccggcc aggctgccgg cgctggacat cgtggtgcag gacttcgagc
    11881 tgcgcggccg gcgcctgggc cgcctggaga tagaggcgcg caaccgcgcc acgcaggacg
    11941 gccagcgtga atggcgcctg ggcaagttca acctggcggc gcccgaggcg gtgttcgcct
    12001 ccagcggcag ctgggccctg gtcggcggca agggcgcagc ggcgcagcgg cgcaccagca
    12061 tggactttgc gctggacata cgcgactcgg gcgcgctgct ggcgcgcttt ggcatggacg
    12121 gcgtgctgcg ccgcggcaag gggcgtatcg ccggccaggt gggctggcag ggctcgccgc
    12181 tgtcgccgga ctaccgcagc atgaccgggc agatgcatgt ggacgtggaa tcgggccagt
    12241 tcctcaaggc cgacccgggt ctggccaagc tgctcggcgt gctcagtctg cagtcgctgc
    12301 cgcggcgcct tgtgctggat ttccgcgacg tgttcagtga gggctttgcc ttcgacttcg
    12361 tgcgcggcga cctgcatgtg gagcagggcg tggccaccac caacaatctg cagatgaagg
    12421 gcgtgaatgc cgcggtgctg atggagggca gcgccgacat cgagcgtgag acgcaggatc
    12481 tgcatgtggt ggtggtgcct gagatcaatg ccatgaccgc ctcgctcgtg gccacggcca
    12541 tcaacccggt cataggcctg ggcagctttc tggcccaggt gtttttgcgc gggccgctga
    12601 tccaggcggc gacgcaagag tttcgcattg acggcacctg ggccgagcca cgcgtggagc
    12661 gcatccagca gcgccaggcg gcgcccggtg ctggcggccc tggcgccccc gcaacctcag
    12721 gagcaaaacc atgaaagtcg cagccttgca aatggtctcc ggcgtggccc tgcagcccaa
    12781 tctggaggtc gcacgccagc tgctgcagca ggcggcccgt gccggagccg agctggcggt
    12841 gctgcccgag tatttctgcg ccatgggccg gcgcgacgag gacaagctgg cgctgcgcga
    12901 gcgcctgggc gatgggccgg tgcagcgctt tctggccgat gcggcgcgcg agctgggcct
    12961 gtggatcgtt ggcggcacgc tgcccctggc gggggacgat ccgcggcgcg tgtacaacag
    13021 ctcgctggcg ttctcgccgg cgggcgaatg cgtggcgcgc tacgacaaga tccacctgtt
    13081 tcgcttcgac aacggccgcg agcagttcga cgaggcacgc accatccagg ccggccaggg
    13141 cagcgtgcgc tttgcgctgc cggcgcgcga cggccggctg tggcatgtcg gtctgtccgt
    13201 ctgctatgac ctgcgctttc ccgagctgta ccgcgaacat gcgcgtgccg gcgccgacct
    13261 gctgctggtg cccagcgcct tcacctacac caccggccgc gcgcactggg agttgctgct
    13321 gcgcgcgcgc gccgtggaga acctggccta tgtgctggcg ccggcccagg gtggtcagca
    13381 cgagaacggc cgccgcacct ggggccacag catgtgcgta gacccctggg gccaggtgct
    13441 ggcgcggcag gccgagggat cgggggtggt gctgggtgag ctcgatgccg cccatctgcg
    13501 ccagctgcgc agccagctgc cggcgctcga gcaccgcgtg ctgtgatgac tgagaccgca
    13561 gcgccacacg agccagccgc gccgccgctg gagcggctgc cagggcgctg gcgccgctgg
    13621 ctgggcgcgt ggcggcgctg gtcgctgtgg gccttgctgc tggcgctggt ggccagcatg
    13681 ctggtcacgc tggtgtggct ggccggccgc tacgaggccg gcgaggtgca aagccggctc
    13741 gagcgcgaca gcgcggacgc cgtggccgac atccgcaacg ccttcgcgca caacctgcag
    13801 aacctgcagg cgctgcaggc gggtgatccc gacctggcga tctgggaaag ccgtgccggc
    13861 gatctgctca cccagcggcg cgagctggtg cgcatagagt ggcgcgacac ccagctgcag
    13921 ctgcgcgcgc atgcccagac gccctaccgt ccccaggggc gcgacccgac ccagcgcgcc
    13981 agcgtgcgct ccgagacagc gctggcctgc gccaacgcgc ggcgcatcac cgggccctcc
    14041 tactccacca gctacttcca gcccatgccc gatggcgtgg gcgccgagat gatggaggtc
    14101 tgcctgccgc tgatgggcga tgggcgcaac catggctttc tggttgccac ctacgccttg
    14161 cacaccgtgc tgcaggagct ggtggcgccg ggcctcaagc gcacccagga ggtgtcgttc
    14221 accgaggccg acggcacgcg cctggcgctg gtgggcgcca tgcgccgcgg cacgcgcatg
    14281 ttcacggcac agcagctgtt cgacctgccg ggcaatacgc tgatcctgcg catggatggc
    14341 tggcatgccg cgcccagcgt gtttcccaac gtgctcacgg ccctggtcac ggccatgtcc
    14401 atcgcgctgg tgtcggtgct ggtggtgctg gtacgcgaca accggcgccg cctgagcgcc
    14461 gagcgtgacc tcggcgatgc gctggcgttt cgcaaggcca tggaggactc cctggtcacc
    14521 ggcctgcgcg cgcgcgacat ccatggccgc atcacctatg tgaatccggc cttttgcgcc
    14581 atggtgggct tctccacgtc cgagctggtg ggcctggaca cgccgcccta ctggccgccc
    14641 gagcatgcgc gggaatatgc cgagcgccag atggtgcgca tggccgggcg gctcgatccg
    14701 ccgcgcgagg gctatgaatc gaccttcatg cgcaaggacg gcacgcgcct gacggtgatg
    14761 atcttcgagg ccccgctgat caacgcccag ggcctgcaca cgggctggat gggctccttc
    14821 ctggatgtga ccgagcagcg ccgcgccgag gaactttcgc gcacctcgca ggagcgcctg
    14881 caggccacgg cgcgcctggc caccgtgggc gaaatggcat cgctgctgag ccacgagctg
    14941 aaccaacccc tggcggccat cgccagctat gcctcgggct cgctgaacct gctgggcccg
    15001 gcgcaggcgg cgcagccgcc gtcggcgcat gacctggccg acctgcgcgt gggcgtgcag
    15061 cgcatcgccg cccaggccga gcgcgccggg cgcgtgatca agagcgtgca cgacttcgtg
    15121 cgccggcgcg accagtcacg cgcgccggcc agcgcacagg ccttgttcga cgccgtgctg
    15181 ccgctgatcc ggctgcaggc gcacaagatt ggcgtgcgcg tggtgctgga catccaggcg
    15241 ggcctgcccc cggtgctgtg cgaccgcacc atggtcgagc aggtgctgct gaacctggcg
    15301 cgcaacgcca tgcagtccat ggacgatccg cagatccacc cgcgcatcct ggacttgcac
    15361 gtgcgccgcg cggcctcgaa cgcggccagg ggctggctgg agttctccgt ggccgacatg
    15421 ggcagcggca tccccgagga cgtggctgaa cgcctgttca cgcccttcta caccacgcgt
    15481 gccgagggca tgggcctggg cctgtccctg tgccgcacgg tggtggagca gcacggcggc
    15541 tttctgggcc atgcccagcg ccagccgcgt ggtacggtat tcaccttcac cctgcccgta
    15601 tttcaggaag actgatggaa cccgccgcca atgccaccgt gtacatcgtg gacgacgacg
    15661 ccgaggtgcg cgaggccctg gcctggctgc tgcgttcgcg ccgcgtgctc agcgaggcct
    15721 atgccagcgc cgaggccttc gagctgatgc tcgccgagcg cgccaccgcc ggcctgccgc
    15781 agcgccagcc ctgctgcctg ctgctggacg tgcgcatgcc cggcatgagc gggctggcgc
    15841 tgttcgacaa catggtcgcg cgtggcgaca ctgccggcat accggtga
//
LOCUS       DAOMTQ010000061        15691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_586_length_15691_cov_26.611857, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000061 DAOMTQ010000000
VERSION     DAOMTQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_15691_cov_26.611857"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..342
                     /locus_tag="PK518_07785"
     CDS             10..342
                     /locus_tag="PK518_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM48852.1"
                     /translation="MPFSAQHLPQAPQREAVDQLRGAAVLEFGTPWCSHCQSAQPLIQ
                     QALQPEGEVQHIKVEDGPGQPLGRSYRVKLWPTLVFLRDGAEVARLVRPQAVQDIRQA
                     LSMLKTAS"
     gene            complement(345..791)
                     /gene="rnhA"
                     /locus_tag="PK518_07790"
     CDS             complement(345..791)
                     /gene="rnhA"
                     /locus_tag="PK518_07790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRM48853.1"
                     /translation="MNQVVIYTDGACKGNPGPGGWGALLRSGALEKELFGGELGTTNN
                     RMELLAVIQALAALKKPCQVALYLDSQYVRQGITEWIHGWKKKGWRTAAGQPVKNVEL
                     WQRLDELAHRAGHRIEWHWVKGHAGDPGNERADALANKGVEQALGR"
     gene            complement(828..1613)
                     /locus_tag="PK518_07795"
     CDS             complement(828..1613)
                     /locus_tag="PK518_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM48854.1"
                     /translation="MSGENYKLHHWFGTPPGRYLLAWEQERYDELVADIFGYHALQLG
                     MPGLQALRANRMPHRWLALGADEAQGVPPAEGAADQGVPPALLAEAVALPFAEASLDL
                     VALPHTLELSVDPHAALREVYRVLVPEGRTVISGLNPWSLWGLRQGRARLYQRLGGGG
                     QPYLPDVGEFISPGRLRDWLRLLGFELETISFGCYRPAATKERWLQRHAWMESLGARW
                     WPILGAGYVIVAAKRVPGMRLLEPSWRKAPRTAGAQVQVARRH"
     gene            1612..2388
                     /gene="gloB"
                     /locus_tag="PK518_07800"
     CDS             1612..2388
                     /gene="gloB"
                     /locus_tag="PK518_07800"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRM48855.1"
                     /translation="MNLLPLPAFTDNYIWMLHDSREAVVVDPGDAAPVLQALHDKGLS
                     LRGILVTHHHGDHVGGVNTLRAATGAAVYGPAGETMPQPLTRLRQGDNITVLGLRFVV
                     LDVPGHTAGHIAYYCADMDGAPLLFCGDTLFSGGCGRLFEGTPGQMQSSLDQLAALPD
                     ATRVCCAHEYTLSNLKFALAVEPGNQALLDYLGHCNALRQNGQPTLPSRIAIERAINP
                     FLRTREATVAQAARSFDPQLDPHQPAAVLAALRQWKNEFR"
     gene            2385..3911
                     /locus_tag="PK518_07805"
     CDS             2385..3911
                     /locus_tag="PK518_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRM48856.1"
                     /translation="MKILHILGLASLLWLTGCATTNTPGDNTPVPLPAPSDTSTSQAP
                     QYPAGPLRPITAGAAQGHVVAGISAPSDLWDRIRRGFAMPDLETDQVRDREQWYATRP
                     DYMQRMTERSSKYLFHIVEELERRGMPTELALLPYIESAFNPQAISSAKAAGMWQFMP
                     ATGTYFDLKQNAFRDDRRDVLASTRAALDYLQKLHGMFGDWHLALAAYNWGEGSVARA
                     IARNEKQGLGTGYTDLNMPAETRMYVPKLQAVKNIVAEPTRFSTDLPVIHNHPYFQSV
                     EITRDIDVKLVASLAGIRLEDFRALNPSLHKPVILSAGMPQILLPWDNAQVFRKNLAA
                     YNEGQYASWTVWTVPNTMSVTAAAQRVGMDVGDLRQMNGIPPRMMIKAGSALMVPRAA
                     GKGADVSEHVADNGQIAFTPEIVTRRTAVRAGKRDTVASIAQRYKVKAADVADWNDLK
                     LTGKFKAGEKVVMYLPVRLTAIAQARTGARPAAGKAPAHTTAKVSPKRKGGTPSKRKR
                     "
     gene            complement(3988..4800)
                     /locus_tag="PK518_07810"
     CDS             complement(3988..4800)
                     /locus_tag="PK518_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRM48857.1"
                     /translation="MTDSSTPAPAAAPPAPLVLKPLGFRDPFRWLAGGVRDLLAAPGI
                     AAFYGACFWGMALVLGWVFRSQPEYTMSLASGCLLVGPFLAMGLYETSRRREAGLPPD
                     LGVSITCWDRHVSSMSMLVLVLIVLELLWGRASLVVFAVFFNTGMPSTTGVMQAVFNP
                     QNWQFVVVYLLVGGVFAALVFSSMVVSIPMILDRDTDALTAGIASIRVVLENPGVMLL
                     WGALITLLVALALAPWGAGLLVVGPMLGHASWHAYRAAVAAPAAVEAAEPEA"
     gene            complement(4919..6145)
                     /locus_tag="PK518_07815"
     CDS             complement(4919..6145)
                     /locus_tag="PK518_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM48858.1"
                     /translation="MTQAPAADLSTRLIHHDYTPPAGFEAPQPPVHKASTVIFPNVSA
                     LRAREWKDKSSYTYGLHGTPTTYQLEERLATLEGGLQCLLVPSGLAAIATVSLALLRQ
                     GDEVLIPDNAYGPNKALAEVELRHYGIAHRIYDPLDPADLAAKITDATKLVWLEAPGS
                     VTLEFPDLPDQVRICRARGVTSVLDNTWGAGLAFAPFDLMGDGQLGADISVHALTKYP
                     SGGGDVLMGSITTRDEALHLRIKLTHMRLGLGVGANDVEAVLRSLPSMALRYHAHDLA
                     ARELARWMGTQAPIAQVLHPALPGSPGHDHWQALCGGAQGGQGAAAGLFSVVIDARHS
                     QEQVDAFCDGLRLFKIGYSWGGPMSLVVPYDLASMRARATPQLKPGTVVRFSIGLEAV
                     QDLRQDLAQAMERAFA"
     gene            6214..7890
                     /locus_tag="PK518_07820"
     CDS             6214..7890
                     /locus_tag="PK518_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRM48859.1"
                     /translation="MPTPVLHRWFPFLAWPRPSGELLQGEFWAGMTVGLMLVPQGVAY
                     AALAGMPLVTGIYASLVPALVAVLWSSSTRLGVGPTALTSLLIGASLTGMAEPGSAHW
                     VALAAWMALLSGLLQLTLGVVRFGWLLNLVTSPVLGGFTQAAALLILASQLPALLGLR
                     TSWSALASSPSLQHFDFTALAFGLGSWAFLVLAKRWRSSFPAAIVLIALAALISWAID
                     YADTDGAVIGHLPSGLPSPYWPGWLSWHELGTLVMPVMVVTLVSFLETASSAKVEHQQ
                     GGTRWNENQDLIAHGLAKMSSGLCGSFATSASFSRSAVNLYAGAKTGWATLFALLLVL
                     ATLLWLTPALYHVPQSVLAAVVVTAVTNLIKPRTMFQLWRISRVETVIGLATFGLTLA
                     TAPRLYWGVLVGILMNLSHFLFQRLHPRIIEVGLHADGSLRDRNLWQLPPIAPRVLAL
                     RMDAELDFASANALERRVADELAQRPDLLHLCLFALPINRIDVTGVEAFMRLRALLQS
                     QGGMLHVAGIKLPVQRRLESAGALVPGPNFVTYRTDADAVAALKTTLSAP"
     gene            7982..8755
                     /locus_tag="PK518_07825"
     CDS             7982..8755
                     /locus_tag="PK518_07825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM48860.1"
                     /translation="MTDAHPSASIRLRDLQLATAHALVAQSLGSEQALREQVPLQYLQ
                     NLIDGLCELSLKDPLTGLPNRRHVGAVLEREIDRVARSGEGALLLMLDIDHFKRVNDS
                     HGHLAGDIVLQSVARTLESCVRPMDTVARYGGEEFAIVLPACQFGFGRVVAERMRRAI
                     EATPVRISPSLALNVTVSIGGAFALQWIRSTTNLWIDRADAELYKAKLAGRNRVSIEE
                     QPDSSVSAEEKSLLFGPLYTPSGWGDLPPPVDAPTGSAH"
     gene            8766..9632
                     /locus_tag="PK518_07830"
     CDS             8766..9632
                     /locus_tag="PK518_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRM48861.1"
                     /translation="MNDALSPSTNSTTGTPATEAAGKPTANPPVDARIIAITSGKGGV
                     GKTFVSANLAAALTRRGHRVLVLDADLGLANLDVVLNLHPKVTLHDVFTGKARLDDAV
                     IQATGGFSVVLAGSGMVEYSRLTPEVRNELFNIIQTLAPRYDVVLLDTGAGISDVVLF
                     SVSLAQEVLIVATPEPTSLTDAYAAIKVLATQQKRQQVRLVVNQAARPGDGRAITGQL
                     QQVLDRFVTTDTGRPMRLVHMGDIPADPSVREAVMRRQLLMQQMPGCPAALAVAQLAN
                     KIESTLLKPAGQ"
     gene            complement(9682..10611)
                     /locus_tag="PK518_07835"
     CDS             complement(9682..10611)
                     /locus_tag="PK518_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRM48862.1"
                     /translation="MSSSTTVVFADMAGSTALFEALGNEQAAALVTRLTQGIGVCVQD
                     HGGRVIKKLGDGVLGVFAQPGHAVAASVALMREHRQWLQSQPQALRVEIHVGLASGEV
                     VEVDGDCYGDAVNVAARLCDLAGGAEVWATQAVVAGLVPARGVGFIRLGHVEVRGKAE
                     AQVMYQVEWREDAEADCTMHSDLMSELNRIDDQPVAEIQLSGPGGQRLRASSGSPVQL
                     GRAADSDLCLIDPRVSRVHARLEWRQGAFVLTDLSSFGTWVRFAGSETAVQLRRDNCL
                     LHGSGEIALGVSFADGNAPIIGFQVSDSGTRPV"
     gene            complement(10859..11836)
                     /locus_tag="PK518_07840"
     CDS             complement(10859..11836)
                     /locus_tag="PK518_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRM48863.1"
                     /translation="MSQDHDHHHCGHDHGPRHIYIYSPSGAVRDKAAFRRGIKRLQAM
                     GHEVEVDQDALASHTRFAGDDATRLAAIHRAAASGADVALISRGGYGITRILPGIKYK
                     ALAKAVERGTQFVGVSDFTALQLALLARTGATTWAGPALVGDLGVEGEPDDIMLACLD
                     DLFTGHGEGSGWRMHAEKPLADGRPAVRDIYVKKACLWGGNLAVLCSLVGTPYLPQIE
                     GGVLFLEDVGEHPYRIERLLTQLLHAGVLSRQKAVVLGQFNGYQLAAHDKGFKLQSVV
                     DWLRTQIKAPVLSNLPFGHVPTKVLLPVGAEVSLSVEGRDALIYWGHAH"
     gene            complement(11908..13038)
                     /gene="tadA"
                     /locus_tag="PK518_07845"
     CDS             complement(11908..13038)
                     /gene="tadA"
                     /locus_tag="PK518_07845"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRM48864.1"
                     /translation="MSKLDDTSWMQAALVEARAAQQAGEVPVGAVLVRDGQVIASGRN
                     APIACHDPTQHAEMAALRVAAQQLGNYRLDGCTLYVTLEPCAMCAGAMLHARLPRVVY
                     GAADPKTGAAGSVVNLFAEPLLNHQTQIEGGVLAEECGALLQNFFSERRRAQRARRLA
                     AHPLRDDALRTPESAFADLPGYPWAAHHVSDLPALAGLRLHYLDEGPRQAPRSWLLLH
                     GGAGWSYQYRHLLAALVEAGERVLVPDLIGFGKSDKPKQQAAHGQAWHVQVLLELMQR
                     LDLRHVVLVEQGGTWHQLAQAAPERIAGLLTLQVPAPQGLAYRLPFPDAGHRAGPRAF
                     AGWVVDRGSLPAQLPRLAERCALDQPAEARALAARAVEYFRL"
     gene            complement(13086..13322)
                     /locus_tag="PK518_07850"
     CDS             complement(13086..13322)
                     /locus_tag="PK518_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48865.1"
                     /translation="MIWGFIGLLIALAIVAMLVKKQIGATRIAVPPAVQGQGAPIDGA
                     APTARTQGQQVQQQIRQQLDTLMQQPRALPDDTQ"
     gene            13461..15446
                     /locus_tag="PK518_07855"
     CDS             13461..15446
                     /locus_tag="PK518_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM48866.1"
                     /translation="MSLSPHLLELLSPARDADIGMAAINHGADAVYIGGPAFGARATA
                     GNDIRDLERLIAHAHRFNSRIFITLNTILRDDELEDARRMAWQVYEAGADALIIQDMG
                     LLELDLPPIQLHASTQTDIRTPEKARFLQDAGLSQIVLARELDLAQIAAIRAATDPAR
                     TTIEFFVHGALCVAYSGQCFISHAHTGRSANRGDCNQACRLPYEVLDGAGRIIAHEKH
                     VLSMKDNNQSDNLRALIDAGVRSFKIEGRYKDLGYVKNITAHYRKLLDEIIEEGEFAR
                     ASSGRTTFSFTPDPDQNFNREFTDYFVNGRKDDIGAFDTPKTPGRAIGWVTQLGDKWF
                     ELEVSDKATMLHNGDGLCYYDLQKELVGVHINRAESVSAKKGIWRVFPKNDMAEFKDL
                     RKGLEINRNRDMDWVRTLEKKSSDRRIGLWAALTETADGFALTLTDEDGFVGSASLAQ
                     AHQAATDAAKAEATLREQLGRFGTTIFEINDISLRMNQPWFVPASSLNQLRRDAVAQL
                     EAARAKGFIRLPRALPVEPPTPFPEDTLSYLANVYNQKAHDFYMKHGVKVIDAAYESK
                     EEEGEVSLMITKHCVRYSMSLCPKQAKGVIGVKGTIKAEPLLLINGKEKLKLVFDCKP
                     CEMHVVGRMKKSVIHQHQKDMLAQPMQFYRTRPQISP"
     gene            15523..>15691
                     /locus_tag="PK518_07860"
     CDS             15523..>15691
                     /locus_tag="PK518_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM48867.1"
                     /translation="MRIETLAVHAGYTPEPTTKSVAVPIYQTVAYAFDSAQHGADLFD
                     LKVPGNIYTRIM"
BASE COUNT         2859 a         5538 c         4828 g         2466 t
ORIGIN      
        1 tgaactggaa tgcccttcag cgcccaacac ctgccgcaag cccctcaacg cgaggcagtg
       61 gatcaactgc gaggcgccgc cgtgctggag ttcggcacgc cctggtgcag ccattgccaa
      121 agcgcccagc cgctgatcca gcaggcattg cagcctgagg gggaggtgca gcacatcaag
      181 gtcgaggacg gcccgggtca gccgctgggc cgcagctacc gcgtcaagct ctggcctaca
      241 ttggttttct tgcgcgacgg cgcggaagtc gcccggttgg tgcgtccgca ggcggtgcag
      301 gacatccgcc aggcgctgtc gatgctcaag accgccagct gaacctacct gcccagcgcc
      361 tgctccacac ctttgttggc cagcgcgtcc gcgcgctcgt tgcctggatc gcccgcatgg
      421 cccttgaccc aatgccactc gatgcgatgg ccggcacggt gcgccagctc gtccaggcgc
      481 tgccatagct cgacgttctt caccggctgg ccggctgcgg tgcgccagcc ctttttcttc
      541 cagccgtgta tccactcggt gatgccctgg cgcacgtact ggctgtccag gtacagggcc
      601 acctggcagg gttttttcag ggccgccagg gcctgtatca cggccagcag ctccatgcgg
      661 ttgttggtgg tgcccagttc gccgccaaac agctcttttt ccagcgcacc cgaacgcagc
      721 aacgcgcccc agccaccagg gcccggattg cctttgcagg caccatcggt atagatcacc
      781 acctgattca aaaaagactc ctcaacaaac aaaccattca tccatgatca atgccggcgc
      841 gccacctgca cctgcgcgcc ggccgttcgg ggcgccttgc gccacgaagg ctccagcaga
      901 cgcatgcccg gcacgcgttt ggcggccacg atcacgtaac cagccccgag tatcggccac
      961 cagcgtgcac ccaggctctc catccaggca tgccgctgca accagcgctc cttggtcgcc
     1021 gcaggccgat agcagccaaa gctgatggtc tccagctcaa agcccagcag acgcagccag
     1081 tcgcgcaggc gcccgggcga gatgaattcc cccacatcgg gcaggtatgg ctgcccccca
     1141 ccaccaaggc gctggtacag ccgcgcacgc ccctggcgca ggccccacag gctccacggg
     1201 ttcaagccgc tgatgaccgt ccggccctcg ggtaccagca cgcggtacac ctcgcgcagc
     1261 gcggcatgcg gatccacgct cagttccagg gtgtgtggca gggccaccag gtccaggctg
     1321 gcctcggcaa acggcagggc cacggcctcg gcaagcaagg cggggggtac cccctggtca
     1381 gcggcgccct ccgccggtgg cacgccttgt gcctcgtccg cgcccaatgc cagccagcga
     1441 tgcggcatgc ggttggcgcg cagcgcctgc aggccgggca tgcccagctg caaggcatgg
     1501 tagccaaaga tatcggccac cagctcgtca tagcgctcct gctcccaggc cagcagatag
     1561 cgccccggag gggtgccaaa ccagtggtgc aacttataat tttcgccgct catgaacttg
     1621 ctgccgctgc ccgcctttac cgacaactac atctggatgc tgcatgacag ccgcgaagcg
     1681 gtcgtcgtgg atccaggcga cgccgcgccg gtattgcagg cgctgcacga caagggattg
     1741 tcgctgcggg gaattttagt cacgcaccat cacggcgacc atgtcggcgg tgtgaacacc
     1801 ctgcgcgcgg ccaccggtgc tgccgtctac ggccccgccg gcgagaccat gccccagccc
     1861 ctcacacgcc tgcgccaggg tgacaacatc accgttctgg gcctgcgctt tgtggtgctg
     1921 gacgtaccag gtcacaccgc ggggcatatt gcctactact gcgccgatat ggacggcgcg
     1981 ccactgctgt tttgcggcga tacgctgttc tcgggcggct gtggccggtt gttcgaaggc
     2041 acgcccgggc agatgcagtc ctcgctcgac caactcgctg cattgccaga cgccacgcgc
     2101 gtctgctgcg cccacgagta caccctgtcc aacctgaagt tcgcattggc cgtggagcct
     2161 ggcaaccagg cgcttctcga ctacctcggc cactgcaacg cgctgcgcca gaacgggcag
     2221 cccacgctac cctcgcgcat cgccatcgag cgtgccatca acccgtttct gcgtacgcgc
     2281 gaagccaccg tggcccaggc ggcgcgcagc ttcgacccgc agctggatcc gcaccaaccc
     2341 gcggccgtac tggccgcact gcgtcaatgg aaaaacgaat tccgatgaaa attctgcaca
     2401 tcctggggct ggctagcctg ctgtggctga ccggttgcgc caccaccaac accccaggcg
     2461 acaacacccc cgtccccctc cccgccccca gcgacaccag caccagccag gcaccgcaat
     2521 accccgctgg cccgctgcgc cccatcaccg ccggcgcggc ccagggccat gtcgtggccg
     2581 gcatcagcgc gccctccgat ttgtgggatc gcatacgccg cggctttgcc atgcccgacc
     2641 tggaaaccga ccaggttcgt gaccgcgagc agtggtatgc cacccggccc gactacatgc
     2701 agcgcatgac agagcgctcc agcaaatacc tgttccacat cgtcgaggag ctggagcgcc
     2761 gtggcatgcc caccgaactg gccctgctgc cctatatcga gagcgccttc aacccccagg
     2821 ccatctccag cgccaaggct gccggcatgt ggcagttcat gcccgccacc ggcacctact
     2881 tcgacctcaa gcaaaacgca tttcgtgacg accggcgcga cgtgctggcc tccacccgtg
     2941 cagcgctgga ctacctgcaa aagctccacg gcatgttcgg tgactggcac cttgccctgg
     3001 ccgcctacaa ctggggcgag ggcagtgtcg cccgggccat tgcccgcaac gagaaacagg
     3061 gcctgggcac cggctacacc gacctgaaca tgcccgcaga aacacgcatg tacgtgccca
     3121 agctgcaggc ggtgaagaac atcgtggccg agccaacgcg gttcagcacc gatctgcccg
     3181 tcatccacaa ccacccttat ttccagagcg tggagatcac gcgcgacatc gacgtgaagc
     3241 tggtagccag cctggccggc atacggctgg aagatttccg cgcgctcaat ccgtcactgc
     3301 acaagcctgt catcctctct gccggcatgc cgcagatcct gctgccctgg gacaacgccc
     3361 aggtgttccg caagaacctg gccgcctaca acgaaggcca gtacgccagc tggaccgtgt
     3421 ggaccgtgcc caacaccatg agcgtcacgg ctgccgccca gcgcgtgggc atggacgtgg
     3481 gcgacctgcg ccagatgaac ggcataccgc cgcgcatgat gatcaaggcc ggctcggccc
     3541 tgatggtgcc gcgtgccgcc ggcaaaggtg ccgatgtctc cgagcatgtg gccgacaacg
     3601 ggcagatcgc cttcacccct gaaatcgtca cccgccgcac cgccgtgcgc gccggcaagc
     3661 gcgacacggt cgccagcatc gcccagcgct acaaggtcaa ggccgccgat gtcgccgact
     3721 ggaacgattt gaagctcaca ggcaagttca aggccggcga aaaggtggtg atgtacctgc
     3781 cggtacgcct caccgccata gcacaggcgc gcactggtgc cagacctgcc gctggcaagg
     3841 cgcccgctca caccaccgcc aaggtcagcc ccaagcgcaa aggcggcaca ccatcgaaac
     3901 gcaaacgctg acgccctgtg caccgcggcg agcgtctgcg gttcgcactg ccgcccgggc
     3961 tggcacccgg gccttgaagg ccagggttca agcctccggc tctgccgctt cgacggccgc
     4021 cggcgctgcc accgccgcac ggtaggcatg ccagctggcg tgccccagca tcggccccac
     4081 caccagcaag ccagcccccc agggcgcaag ggcaagcgcc accagcagcg tgatcagcgc
     4141 cccccacagc agcatcacac ccgggttctc cagcaccacg cggatgctgg cgatgccggc
     4201 cgtaagcgca tccgtgtcgc gatcaagaat catgggaatg gacaccacca tgctggagaa
     4261 caccagggcc gcaaacaccc cgcccaccaa caggtagacg acgacgaact gccagttctg
     4321 cgggttgaac accgcctgca tcacccccgt ggtggacggc atgccggtat tgaagaacac
     4381 ggcaaacacc accagcgatg cccgccccca cagcagctcc agcacgatca gcaccagcac
     4441 cagcatgctc atgctggaca catgccgatc ccagcaggtg atggacaccc ccagatccgg
     4501 cggcaggccg gcttcacgcc tgcggctggt ttcatacagg cccatggcca gaaacggccc
     4561 caccagcagg cagccgctgg ccagcgacat ggtgtactca ggctgcgagc gaaacaccca
     4621 gcccagtacc aacgccatgc cccaaaagca cgcaccgtag aaggcggcga taccgggcgc
     4681 ggccaacagg tcgcgcacac cgccggcaag ccagcgaaac ggatccctga aacccagggg
     4741 cttgagcacc agtggtgccg gcggcgcagc agcgggcgcg ggcgtggaag agtcggtcat
     4801 gacggtgggc ccgctgtgcg gtggctgtga cgtagccagc aaccataccc agcgaaaccc
     4861 ttgtcgcgca tcaaggaaac taccacccca tgatggtcac gcttgcgccc gtgcaagctc
     4921 aggcgaacgc gcgctccatg gcctgcgcca gatcctgacg caaatcctgc accgcctcca
     4981 ggccaatgga gaagcgcacc accgtgccgg gtttgagctg cggcgtggcg cgcgcgcgca
     5041 tgctcgcgag gtcatagggc acgaccaggc tcatcgggcc gccccagctg tagccgatct
     5101 tgaacaggcg taacccatca cagaacgcgt ccacctgctc ctggctgtgg cgggcatcga
     5161 tgaccacgct gaacaaccca gcggcagcgc cctggccgcc ctgcgcaccg ccgcacagcg
     5221 cctgccaatg gtcatgcccg ggcgagccgg gcagcgccgg gtgcagcacc tgggcgatcg
     5281 gcgcctgggt gcccatccac cgcgccagct cgcgcgccgc caggtcgtgc gcgtggtagc
     5341 gcagcgccat gctgggcagg gagcgcagca ccgcctccac atcattggcc cccacgccca
     5401 ggcccaggcg catatgcgtg agcttgatgc gcagatgcag cgcctcgtcg cgcgtggtga
     5461 tgctgcccat gagcacatcg ccgccgccac tggggtattt ggtcagggca tgcacggaaa
     5521 tatccgcgcc cagctgcccg tcacccatca aatcaaaggg tgcgaaggcc aggcccgcgc
     5581 cccaggtgtt gtccagaacg ctggtgacgc cgcgcgcgcg gcagatgcgc acctggtcgg
     5641 gcagatcggg aaactccagc gtcaccgagc cgggcgcctc cagccacacg agtttcgtgg
     5701 cgtcggtgat cttggcggcg aggtcggcgg gatcgagcgg gtcatagatg cggtgcgcaa
     5761 tgccgtagtg gcgcagctcg acctcggcca gggccttgtt cgggccatag gcgttgtcgg
     5821 ggatcagcac ctcgtcgccc tggcgcagca aggccagcga gacggtggca atcgccgcca
     5881 gaccgctggg caccagcagg cattgcaggc cgccctccag ggtggccagg cgctcctcca
     5941 gctggtaggt ggtaggggtg ccatgcaggc cataggtata gctgctctta tccttccatt
     6001 cgcgcgcgcg cagcgcagac acgttgggaa agatcaccgt ggacgccttg tgcacgggcg
     6061 gctgcggcgc ctcgaagccc gcgggcggcg tgtagtcgtg gtggatgaga cgggtggaca
     6121 ggtcggctgc gggtgcttga gtcatgtgca gattattagg gcaacgaccg tttccacagc
     6181 aggcccacgc gcatggcgtg acaatcgccc accatgccca cacccgtcct gcaccgctgg
     6241 ttcccctttc tcgcatggcc acgcccctcg ggcgagctgc tgcaagggga gttctgggcc
     6301 ggcatgaccg tgggcctgat gctggtgccg cagggcgtgg cctatgcggc gctggcgggc
     6361 atgccgctgg tgacgggcat ttacgcctct ctggtgccgg ccctggtggc cgtgctctgg
     6421 agttcgtcca cccgcctggg cgtgggcccc acggcgctga ccagcctgct gataggcgcg
     6481 tcccttactg gtatggccga gcccggcagc gcccactggg tggcgctggc ggcatggatg
     6541 gccttgctgt cggggctgct gcagctgacg ctgggcgtgg tgcgctttgg ctggctgctg
     6601 aatctggtca catcgcccgt attgggcggt ttcacacagg ccgcggcgct gctgatcctg
     6661 gcatcgcaat tgccagcgct gctgggcctg cgcaccagct ggagcgcact ggccagcagt
     6721 ccatcgctgc agcattttga cttcacggcg ttggcttttg gcctgggcag ttgggccttt
     6781 ttggtgctgg ccaagcgctg gcgttccagc tttcccgccg ccatcgtgct catcgcgttg
     6841 gcggccctga tcagttgggc catcgactac gcagataccg acggcgcggt gatcggccac
     6901 ctgccttcgg gcctgccctc accctactgg ccgggctggc tgtcgtggca cgagctgggc
     6961 accctggtga tgccggtgat ggtggtcacc ctggtgagct ttctggaaac cgcatccagc
     7021 gccaaggtgg agcaccagca gggcggcacg cgctggaacg agaaccagga tctgatcgcc
     7081 cacggcctgg ccaaaatgag ctcgggccta tgcggcagct ttgccaccag cgcctcgttc
     7141 tcgcgctcgg cggtcaacct gtacgccggt gccaaaaccg gctgggcgac attgttcgcg
     7201 ctgctgctgg tgctggccac cctgctgtgg ctcacgccgg cgctctacca tgtgccgcaa
     7261 tcggtactgg ctgcagtggt ggtgaccgcc gtcaccaacc tgatcaagcc acgcaccatg
     7321 ttccagctgt ggcgcatctc gcgcgtggag acggtgattg gcctggccac ctttggcctg
     7381 acgctggcca ccgcgccgcg cctgtactgg ggggtgctgg tcggcatctt gatgaacctg
     7441 agccactttc tgttccagcg cctgcaccca cgcatcattg aagtcggtct gcacgccgac
     7501 ggcagcctgc gcgaccgcaa cctgtggcag cttccgccca ttgccccccg ggtgctggcg
     7561 ctgcgcatgg acgcggagct cgatttcgcc tccgccaacg cgctggagcg ccgcgtcgcc
     7621 gacgagctgg cccagcggcc ggacctcctg cacctgtgcc tgttcgcgct gcccatcaac
     7681 cgcatcgacg tgaccggcgt cgaagccttc atgcgcctgc gtgccctgct gcaaagccag
     7741 ggcggcatgc tgcatgtggc cggcatcaag ctgcccgtgc agcgccgcct tgaaagtgcc
     7801 ggtgccctgg tgccgggccc caacttcgtc acctatcgca cggacgcaga cgccgttgca
     7861 gcgctgaaaa ccacactctc cgcaccataa aatccgcacc catgatgcag cagcaacccc
     7921 ttgtcaccca agagtgctgg caaggctgct acactgcgat tcattccatt tgtttctttt
     7981 tgtgactgac gcacacccct ccgcttccat ccgcctgcgt gacctgcagc tggccacggc
     8041 ccatgcgctg gtggcccagt cgctgggctc ggagcaggcc ttgcgcgagc aggtgccgtt
     8101 gcagtatttg cagaacctga tcgacggctt gtgcgaactc tcgctcaagg atccgctcac
     8161 gggcctgccc aatcgccgcc atgtgggtgc cgttctggag cgcgagatcg accgcgtggc
     8221 acgctccggc gaaggcgccc tgctgctgat gctggacatc gaccatttca agcgcgtgaa
     8281 tgacagccac ggccatctgg ccggggatat cgtgctgcag tcggtggcgc gcacgctcga
     8341 gtcctgcgtg cgccccatgg ataccgtggc ccgctacggc ggcgaagagt tcgccatcgt
     8401 cctgcccgca tgccagtttg gctttggccg cgtggtcgcc gagcgcatgc gccgcgccat
     8461 cgaggccacg ccggtgcgca tatcgccctc gctggcgctg aacgtcacgg tcagcatcgg
     8521 cggtgccttt gcattgcaat ggatacgcag caccaccaac ctgtggatag accgcgcgga
     8581 tgccgagctg tacaaagcca agctcgccgg gcgcaaccga gtcagtatcg aagaacaacc
     8641 cgacagcagc gtcagcgccg aggagaagag tttgctgttt ggccccctgt acaccccatc
     8701 cggatggggc gacctgcccc cacccgtgga tgcacccact ggcagcgccc actgaaagtc
     8761 aagcgatgaa cgacgcgctg tccccttcaa cgaactccac caccggtacg cccgcaaccg
     8821 aggccgccgg caagcccact gccaacccac cggtcgatgc ccgcatcatc gccatcacca
     8881 gcggcaaggg cggcgtgggc aagacctttg tctccgccaa cctggcggcg gccctgacgc
     8941 gccgtggcca ccgcgtgctg gtgctggatg ccgacctggg cctggccaat ctggacgtgg
     9001 tgctgaacct gcaccccaag gtcacgctgc acgatgtgtt caccggcaag gccaggctgg
     9061 atgacgccgt catccaggca accggagggt tttcggtggt gctggccggt tccggcatgg
     9121 tggagtattc gcgcctcaca cccgaggtgc gcaacgaact cttcaacatc atccagacgc
     9181 tggcgccgcg ctatgacgtg gtgttgctcg ataccggtgc cggcatctcc gacgtggtgc
     9241 tgttttccgt gtcgctggcg caggaagtac tgatcgtggc cacgcccgaa cccacctcgc
     9301 tgaccgacgc ctatgccgcc atcaaggtgc tggccaccca gcaaaagcgc cagcaggtgc
     9361 gcctggtggt caaccaggcc gcgcgcccgg gcgacggccg cgccatcacc ggccagttgc
     9421 agcaggtgct ggatcgcttt gtgaccacgg ataccggccg tccgatgcgg ctggtgcaca
     9481 tgggtgacat cccggccgac ccctcggtac gcgaggccgt gatgcggcgc cagttgctga
     9541 tgcagcaaat gccgggctgc cccgcagccc tggccgtggc ccagctggcc aacaagatcg
     9601 agtccactct gctcaagccg gccggtcagt agtctccagc gccggaaggc caacaacgga
     9661 cgtcagcgca tcaggcgcgc ctcaaaccgg gcgcgtgccg ctgtccgaaa cctgaaaacc
     9721 gatgatgggt gcattgccat cggcaaagga cacgcccagc gcgatctccc ccgaaccatg
     9781 cagcagacag ttgtcgcggc gcaactgcac ggctgtctca ctgccggcga aacgcaccca
     9841 ggtgccaaag ctgctcagat cggtaagcac aaaggctccc tggcgccatt ccaacctggc
     9901 gtgcacgcgc gataccctgg gatcgatcag gcacagatct gagtcggccg cacgccccag
     9961 ctgaactggc gatccagacg aagcccgaag ccgctgtcca ccgggcccgg agagttggat
    10021 ctcggcgacc ggctgatcgt ctatccgatt gagctcgctc atcagatcgg aatgcatggt
    10081 gcagtcggcc tctgcatcct cgcgccattc gacctggtac atcacctggg cctcggcctt
    10141 gccgcgcacc tcgacatgcc ccaggcgaat aaagcccacg ccccgcgccg gcaccaggcc
    10201 agccaccacg gcctgggtgg cccacacctc ggcgccgccg gccaggtcac acaaacgggc
    10261 ggccacgttc acggcatcgc catagcaatc accatcgacc tcgaccacct caccactggc
    10321 caggccgaca tggatttcga cccgcagcgc ctgaggctgg gactgcagcc actggcggtg
    10381 ctcacgcatc aaggccaccg acgccgccac ggcgtggcct ggttgggcaa aaacacccag
    10441 cacgccgtcg cccagcttct tgatcacgcg cccgccatgg tcctgcacgc aaacgccaat
    10501 gccctgcgtg agccgcgtca ccaaggcagc ggcttgctca ttgccgagcg cttcaaacag
    10561 cgccgtgctg ccggccatgt ctgcaaacac tacggtggtg ctggaactca cgtcctactc
    10621 cctgtcatgg atatcactgc cggtctgcaa gcccatgtcg cggcatggag cagccaccga
    10681 aggttacaaa ttgcaagaac tttatcttat acgaattaca accaggggca ttcatatgcc
    10741 gccacccctg gctggacatt gacaaacgtt gacacgcaaa taacggccaa gcatgaaccc
    10801 gtcggcaacg cggtacccgg cagggtcacc gccatcgcct gcacaagggc aagaatgctc
    10861 aatgcgcatg cccccagtag atcagcgcgt cacgcccctc aactgacagt gacacctcgg
    10921 cccccacggg cagtagcacc ttggtcggca catggccgaa cggcaggttg ctgagcacgg
    10981 gggccttgat ctgggtgcgc agccaatcaa ccacgctttg cagcttgaaa cccttgtcgt
    11041 gcgcagccaa ctggtagccg ttgaactggc ccagcaccac cgccttttgc ctggacaaga
    11101 caccggcatg caacagctgg gtcagcaggc gctcgatgcg gtaggggtgc tcacccacgt
    11161 cctccaggaa cagcacaccg ccctcgatct gcggcaaata gggcgtgccc acgagagagc
    11221 acaacacggc caggttgccg ccccacaaac aggctttttt cacgtagatg tcgcgcaccg
    11281 cgggtcggcc atcggccagg ggtttctcgg catgcatgcg ccagccgctg ccctcgccat
    11341 ggccggtgaa caagtcatcc aggcaggcaa gcatgatgtc gtccggctcg ccttccaccc
    11401 ccaggtcgcc caccagggcc gggccggccc aggtggtggc gccggtgcgg gccagcagcg
    11461 ccagctgcag cgctgtgaag tcactcacgc cgacaaactg cgtgccgcgc tccaccgcct
    11521 tggccagggc cttgtacttg atgccgggca ggatgcgcgt gatgccataa ccgccgcggc
    11581 tgatcagcgc cacgtcggca ccgctggcgg ccgcgcggtg gatggcggcc aggcgcgtgg
    11641 catcgtcgcc agcaaagcgc gtatggctgg ccagggcatc ctggtccacc tcgacctcat
    11701 ggcccatggc ctgcaggcgc ttgatgccgc ggcggaacgc cgccttgtcg cgcaccgcgc
    11761 ccgaggggga atagatgtag atgtggcgcg ggccatggtc gtggccgcag tggtggtggt
    11821 cgtggtcttg ggacaaggcg gtgtgtcgca cccttgcggg cgcgctccgt ggctggcggg
    11881 cgccatcggc aagaaggcgc ccagaaatca aaggcgaaag tattccacag cgcgtgccgc
    11941 caaggcccgg gcctctgctg gctgatcgag cgcacagcgt tccgccaggc gtggcaactg
    12001 cgcgggcagg gagccacgat ccaccaccca tccggcaaag gcacgcggcc cggcacggtg
    12061 gccagcgtcg ggaaatggca agcggtaggc aaggccctgc ggcgccggca cctgcagtgt
    12121 cagcaagccc gcaatgcgct caggcgcggc ctgggccagc tggtgccagg tgccgccctg
    12181 ctccaccagc accacatggc gcaggtcgag ccgctgcatc aactccagca gcacctggac
    12241 atgccaggcc tggccatgcg cggcctgctg cttgggcttg tcactcttgc caaagccgat
    12301 caggtcaggc accagcacgc gctcgccggc ttcgacaagg gcggccagca ggtgacggta
    12361 ttggtagctc cagccagcgc caccatgcag cagcagccag ctgcgcggcg cctgcctggg
    12421 cccttcgtcg aggtaatgca ggcgcagccc agcgagtgcc ggcaggtcgc tgacatggtg
    12481 cgccgcccag ggatagccgg gcaggtcggc aaacgcgctc tcgggcgtgc gcagcgcatc
    12541 gtcgcgcagc gggtgggcgg ccagccgccg cgcacgctgg gcgcggcggc gttcactgaa
    12601 aaaattctgc aagagcgccc cgcactcctc ggccagcacg ccgccttcga tctgggtctg
    12661 gtggttgaga agcggctcgg caaacaggtt caccaccgaa cccgccgcgc ccgtcttcgg
    12721 gtcggcggcg ccatagacca cgcgcggcag ccgcgcgtgc agcatggcgc cggcgcacat
    12781 ggcgcagggc tccagcgtga cgtagagcgt gcagccgtcc agccggtaat tgcccagctg
    12841 ctgcgccgcc acgcgcaggg ccgccatctc ggcatgctgc gtggggtcgt ggcaggcaat
    12901 cggggcgttg cgcccgctgg cgatcacctg gccatcgcgc accagcaccg cgcccacggg
    12961 cacctcgccg gcctgctgcg ccgcgcgcgc ctcgaccagg gccgcctgca tccagctggt
    13021 gtcatcgagt ttactcattt atttatagct tatagcgctt atacataaag cgctagaggc
    13081 atattttact gagtatcgtc cggcagggcc cgtggctgct gcatgagcgt gtccagctgc
    13141 tgccggatct gctgctgcac ctgctggcct tgggtacgcg cggtgggcgc cgcgccgtcg
    13201 atgggcgcgc cttgcccctg cacggccggc ggcacggcga tgcgtgtagc gccgatttgc
    13261 ttcttcacca gcatggcgac gatggccaag gcaatcaaca gtccaatgaa tccccagatc
    13321 atgcgcatcg ctcgtccctc ccgtatccgc tgccaactcc acgccaggcc tggacccgcc
    13381 aattgcagcc ctggccgcat gggcgcagct ggcatggggg caaggcagga atgtagccgc
    13441 actgggataa tccgacccct atgtccctga gtccccacct gctagaactg ctctcccccg
    13501 cgcgcgacgc cgatatcggc atggccgcca tcaaccacgg cgccgacgcc gtctacattg
    13561 gcggcccggc cttcggcgcg cgcgccacgg cgggcaacga catccgcgac ctggaacggt
    13621 tgattgcgca cgcgcaccgc ttcaacagcc gcatcttcat caccctcaac accatcctgc
    13681 gcgacgacga gctggaggac gcacgccgca tggcctggca ggtctatgag gccggggccg
    13741 acgcgctcat catccaggac atgggcctgc tggaactgga cctgccgccg atccagctgc
    13801 atgccagcac ccaaaccgac atccgcacgc ccgagaaggc gcgttttctg caggacgcgg
    13861 gcctgtcgca gatcgtgctg gcacgcgaac tcgacctggc gcaaatcgcc gccatccgcg
    13921 ccgccaccga ccccgccaga accaccatcg aatttttcgt gcacggcgcg ctgtgcgtgg
    13981 cctattcggg ccaatgcttc atcagccacg cccataccgg ccgtagcgcc aaccgcggcg
    14041 actgcaacca ggcctgccgc ctgccctacg aggtgctgga cggcgccggc cgcatcatcg
    14101 cgcacgaaaa acatgtgctg tcgatgaagg acaacaacca gagtgacaac ctgcgcgcac
    14161 tgatcgacgc cggcgtgcgc agcttcaaga tcgagggtcg ctacaaggat ctgggttatg
    14221 tgaagaacat cacggcgcac taccgcaagc tgctcgacga gatcatcgaa gaaggcgaat
    14281 ttgcgcgcgc atcgagcggg cgcaccacct tcagcttcac acccgacccg gatcagaact
    14341 tcaaccgcga gttcaccgac tacttcgtca atggccgcaa ggatgacatc ggcgccttcg
    14401 acacgcccaa gaccccgggc cgcgccattg gctgggtcac ccagctgggc gacaagtggt
    14461 tcgagctgga ggtaagcgac aaggccacca tgctgcacaa cggcgatggc ctgtgctact
    14521 acgacctgca aaaagagctc gtcggcgtac acatcaaccg cgccgaaagc gtgagcgcca
    14581 aaaagggcat ctggcgcgtc ttccccaaga acgacatggc cgagttcaag gatctgcgca
    14641 agggcctgga gatcaaccgc aaccgcgaca tggactgggt gcgcacgctg gagaaaaaat
    14701 ccagcgaccg gcgcataggc ctgtgggcgg cactgacgga gaccgccgac ggctttgccc
    14761 tgacgttgac cgacgaggac ggcttcgtcg gcagcgccag cctggcgcag gcgcaccagg
    14821 ccgccaccga cgccgccaag gccgaagcca cgctgcgcga acaactgggg cgctttggca
    14881 ctacgatttt tgagatcaac gacatctccc tgcgcatgaa ccagccctgg ttcgttcccg
    14941 cctccagcct gaaccagttg cgccgggacg ccgtggccca gcttgaagcc gcccgcgcca
    15001 agggtttcat ccgcctgccg cgcgccctgc cggtcgaacc gcccacgcca ttccccgaag
    15061 acacgctgag ctacctggcc aacgtctaca accaaaaggc gcacgacttc tatatgaagc
    15121 acggcgtgaa ggtgatagac gcggcgtatg aaagcaagga agaagaaggc gaggtcagcc
    15181 tgatgatcac caagcactgc gtgcgctaca gcatgagcct gtgccccaag caggccaagg
    15241 gcgtcatcgg cgtcaagggc accatcaagg ccgagccctt gctgctcatc aacggcaagg
    15301 aaaagctcaa gctggtcttt gactgcaagc cctgcgagat gcatgtggtg ggcaggatga
    15361 aaaagtccgt catccaccag caccagaagg acatgctggc ccagcccatg cagttctacc
    15421 gcacgcggcc gcagatcagc ccatagccag gcattgatcc gggcaggccg cgccttgacc
    15481 gacaatgtcc ggttgctaca acccaaccca aggaagacac cgatgcgcat cgaaaccctg
    15541 gccgtccacg ccggctacac gcccgagccc accaccaagt ccgtggccgt acccatctac
    15601 cagacggtgg cctatgcctt tgacagcgcc cagcatggcg ccgacctgtt cgacctgaag
    15661 gtgccgggca acatctacac gcgcatcatg a
//
LOCUS       DAOMTQ010000062        15564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_596_length_15564_cov_26.932039, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000062 DAOMTQ010000000
VERSION     DAOMTQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_596_length_15564_cov_26.932039"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PK518_07865"
     CDS             <1..666
                     /locus_tag="PK518_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HRM48868.1"
                     /translation="RREPAAPASAPEGAGEQRPPQPQPRRTHPLLEQLAGWYPHLFGA
                     QFLPLKRGIFHDLMAAHGEAIDKDALKLALAIHTRSTRYLNAVAQGMKRHDLQGQAVE
                     DMAPEHVHHALLEVFRRRKPRDGEDLTATLRRRIAQAFEASGLTREAYDALVRGRDDK
                     ANALLDEAFAEVAERDAKAEALARAFEASGATPEQFADMYGMDRRAVTQSLARARRPQ
                     AKG"
     gene            678..1193
                     /locus_tag="PK518_07870"
     CDS             678..1193
                     /locus_tag="PK518_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HRM48869.1"
                     /translation="MWLGVSLFYVGAVLCLNGLWMRGHIADREIWVINVFVGLVSLAI
                     GLFSIFGPGADAHSIQGGALTLLFAFTYLWVAANRFSGADGRGLGWFSLFVAITAVPV
                     ALDTLAGARSLLDWWMGANWAAWALLWALFWLLLARGRDIGRFTGAMCIAQGVLTGWL
                     PGYLLLSGRLY"
     gene            1537..2301
                     /locus_tag="PK518_07875"
     CDS             1537..2301
                     /locus_tag="PK518_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRM48870.1"
                     /translation="MSKKLGSMAAAILMTLGTSAYAGAFVNGGFEDGNLNGWTGGGGS
                     WFGGGAPVDPATYAGGPSNNTIMIGGTDPITGANRVYGGGYSVRVNDEVNNRSVSTIS
                     QTVNNYTDNSIFFAWNAVLEDSHGLTDSDYFSLTLRDKTTGTNLVSRAYSSAGSIGSG
                     TSGVTWTKFGNWYSSGWVVEQIDLAALGAVGHDFTLTLLASDCPYSGHAGYVYLDGFG
                     AVNPPTGQVPEPGSLALLGLGFAGLAAARQRQKKAA"
     gene            complement(2405..3280)
                     /locus_tag="PK518_07880"
     CDS             complement(2405..3280)
                     /locus_tag="PK518_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM48871.1"
                     /translation="MQSPLPVLALLCNALLWGLSWWPFRSMHAAGLHPLWATAVMYTL
                     VCAVMLAARPGSLGDLLRQPALWLLALNSGINNMAFNWAVTVGDVVRVVLVFYLMPAW
                     LVLLAWRILGERPTPAALARLGLAFGGVALVLVPAGASWGGLLANLSLADALALLGGF
                     TFAVTNVLLRRLHTVPGPARMLAMFAGCMGLGWLAALAGLAAGVVPAFPAFDPRWAAM
                     AAALALLLMLGNWALQYGAARLPTAITGVVMLSEVLFASVSSVLLGAAQLQPRTLAGG
                     ALIVLAALWATLQRR"
     gene            complement(3466..4743)
                     /gene="lplT"
                     /locus_tag="PK518_07885"
     CDS             complement(3466..4743)
                     /gene="lplT"
                     /locus_tag="PK518_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HRM48872.1"
                     /translation="MKRGFYTIMSAQFFSSLADNALFVTAVELLRSGGSPEWQRAALV
                     PMFALFYVVLAPFVGAFADSMPKGRVMFISNAIKVVGCLMMLFGSHPLLAYGVVGLGA
                     AAYSPAKYGILTELLPASQLVKANGWIEGLTIGSIILGVVLGGQLVAPHVSGLLLGLD
                     MPGIDLGLDLPAEAAILALVPVYVLAAAINLRIPLTGVAMRPMPRQLLVLLRDFWTCN
                     NRLWRDRLGQISLSTTTLFWGVSGNLRYIVLAWAGVALGYSTTQASALVGVVALGTAA
                     GAVLASSYTQLDKATRVLPLGVAMGLVVILMNAIDNIWLAVPFLILLGGLGGFLVVPM
                     NALLQHRGHNLMGAGRSIAVQNFNEQAAILGMGVLYSLFTKWGLSAFGAIAAFGLLVA
                     AVMVVILRWHVYNVRHYPKEMVQLRKIARLDQH"
     gene            4886..5980
                     /gene="alr"
                     /locus_tag="PK518_07890"
     CDS             4886..5980
                     /gene="alr"
                     /locus_tag="PK518_07890"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRM48873.1"
                     /translation="MPRPILATIHPAAVQHNLERARRAAPDARVWAVVKANAYGHGIE
                     RVFEALRSADGIALLDLAEAQQLRQLGWRGPILLLEGVFEPRDLELCSRLSLWHVVHC
                     EQQIDWLAAHKTQVPQRVFLKMNSGMNRLGFTPGRYRSAWARLNALPQVDEISFMTHF
                     SDADSARGIAHQLRAFEQATQDLPGERCIANSAAILRHGGAAEVRLDWVRAGIVLYGS
                     APDHPERTAAAWDLQPAMTLASRLIAVQQLQAGDSVGYGSRFTADGPLRIGVVACGYA
                     DGYPRHADTGTPVLVGGVRTRTVGRVSMDMITVDLTPVPDAGPGAEVTLWGRAGNGAL
                     LPIDEVAQAAGTVGYELMCALAPRVPVTMD"
     gene            6131..7156
                     /locus_tag="PK518_07895"
     CDS             6131..7156
                     /locus_tag="PK518_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRM48874.1"
                     /translation="MSYPSRSSSLRHVLRFALGAVGAAALLAAAPARAQEPVQPIAPA
                     KVTNPAKVELGKKLWFDPRLSRSGFISCNSCHNLSMGGSDNLKTSIGDKWQKGPINSP
                     TVLNSSLNLAQFWDGRAKDLKEQAGGPIANPGEMAFSHDLAVDMLRSIPQYVGEFKRV
                     FGHDKLGIDEVTAAIAAFEETLVTPNAPFDKWLKGDKKAITAQELRGYQLFKGSGCVA
                     CHNGPNLGGTSFQKMGLVEPYKTDNPAEGRSAVTGNDAERFNFKVPTLRNVELTYPYF
                     HDGAADTLAEAVDTMGRIQLGRKFSDKENADIVAFLKTLTGDQPRIAMPILPPSSDKT
                     KRPQPFE"
     gene            complement(7254..7877)
                     /locus_tag="PK518_07900"
     CDS             complement(7254..7877)
                     /locus_tag="PK518_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRM48875.1"
                     /translation="MPALTLYYAPGTCAQAVRIALEEAQAPHALVRVDFASQQQRTPE
                     YLAVNPKGRVPALVTEQGTLTETPALLVYVAQRFPAAQLAPTDAFGLARMQEFNSYLA
                     STVHIAHAHRPRAARWADEPEAQAAMQRKVPANMTECFDIIERHYLAHTDQGPWVLGA
                     QYSVADGYLFTMAGWLKSDGVDIARFPKVAAHRERMAARPAVQRALA"
     gene            8017..9402
                     /gene="radA"
                     /locus_tag="PK518_07905"
     CDS             8017..9402
                     /gene="radA"
                     /locus_tag="PK518_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRM48876.1"
                     /translation="MAKDKTLYTCSECASTSPRWLGKCPGCGAWNTLIETVPEAAPGK
                     NRLSNAAGQYAGLAQAQAVMPLAAIEATEVARTASGVEELDRVLGGGVVEGGVVLIGG
                     DPGIGKSTLLLQAMDALQRRGLPTLYVTGEESGAQVALRSRRLGLDASQVNVLAEIQL
                     EKILATIEATQPAVAVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRLAKATGIAIIL
                     VGHVTKEGALAGPRVLEHMVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTER
                     GLKGVANPSAIFLSQHAEPVPGSCVLVTLEGTRPMLVEIQALVDGGGPSPRRLSVGLE
                     RDRLAMLLAVLHRHAGVACADQDVFVNAVGGVRISEPAADLAVMLAITSSLRGRALPK
                     GFIAFGEVGLAGEVRPAPRGQERLKEAAKLGFTVAVVPKANAPKKPIPGLQIHPVERV
                     DEAMDAVRGLG"
     gene            9478..9726
                     /locus_tag="PK518_07910"
     CDS             9478..9726
                     /locus_tag="PK518_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRM48877.1"
                     /translation="MSTVRWNIAVSQEVDQSVRIFLAAQGGGRKGDLSRFIEESVRAH
                     LLERAVDQAKTAAAGLGESELTELIDEAVQWAREGGGR"
     gene            9726..10145
                     /locus_tag="PK518_07915"
     CDS             9726..10145
                     /locus_tag="PK518_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRM48878.1"
                     /translation="MRVILDTNVLLGALISPYRPPDLIYRAWRASRFELVTSLEQLDE
                     LRRVSRYPKLKAILPAHRIGTMVNSMQRALVLPMLPPLPEGVQATDPNDTFLLQMAVA
                     SAADYLVTGDRRAGLLQRGSLGRTRIVTPVAFCDEVL"
     gene            10204..10620
                     /locus_tag="PK518_07920"
     CDS             10204..10620
                     /locus_tag="PK518_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRM48879.1"
                     /translation="MNLRKFLIPLGIVVLVIAAWRAYGPQGILTVSGGLVMWGLLHYT
                     RLMNVMQKARHNPIGYVASAVMLNAKLKPGVNLMHVMAMTRALGEQLSAEGQEPEVYR
                     WTDGTKSHVTCKFFNGRLASWELVRPAPGEEPAAES"
     gene            10664..11602
                     /locus_tag="PK518_07925"
     CDS             10664..11602
                     /locus_tag="PK518_07925"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRM48880.1"
                     /translation="MSPVVPSMADRDGKIWMDGQMVDWRDAKIHVLTHTLHYGCGAFE
                     GVRAYNTVNGTAIFRLQEHTDRLFNSAKILRMQLPFSKDEVNQAQIDVVKANNLESCY
                     LRPLTWIGSQKLGVSPKGNQIHLMVAAWAWGAYLGEEGMQRGIRVKTSSYTRHHVNIT
                     MTQAKAVSNYSNSILANMEALDDGYDEALLLDAAGFVSEGAGENIFVVKDGVVYTPDL
                     SAGALNGITRNTVLHICKDLGLDLVQKRITRDEVYIADEAFFTGTAAEVTPIRELDRV
                     QIGAGSRGPITEKIQSAFFDIVNGRNPKYAHWLTKV"
     gene            11609..11815
                     /locus_tag="PK518_07930"
     CDS             11609..11815
                     /locus_tag="PK518_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRM48881.1"
                     /translation="MTQAIVELLAKDLDGNGGVHCPNPKADMKLWNSHPRVFLEIAHQ
                     GHASCPYCGTEYRLKAGEVLKGGH"
     gene            11889..12983
                     /locus_tag="PK518_07935"
     CDS             11889..12983
                     /locus_tag="PK518_07935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM48882.1"
                     /translation="MKVLHFVTGGFSGATQVAVDLCLAARGEPHMQTLLVLRRKRNTS
                     AERVQALRDQGLQVQLVSNWLHALTVWELRKIIRDWRPDVVFAHGFSDHIWGRRAAVA
                     EGVPRIFHVEHNSRERYTPRRLRQALELEPHTQASIGVSEGVRTALIERGFAPGKCLA
                     IPNGIALERFPASLLPQRWEQREPAILMASRFARQKDHTTLIAALGLLRERGLRPTLY
                     LAGAGKAGLRGKAEALAQSLGLQEQVRFLGNVPDLPQRLAATQVFVLSSHWEGMPLAL
                     VEAMAAGCACVGSDVLGVREVLEHGRTGLLVPPAEATALAYALQQLLQNPAQAQRLGQ
                     AARQQALTACGREHMWRRYRALLNAPGGRD"
     gene            12983..14263
                     /locus_tag="PK518_07940"
     CDS             12983..14263
                     /locus_tag="PK518_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRM48883.1"
                     /translation="MDYKAETPFKLTTLATFLLAALAIWVPSGYSYGAVLLLAGALFY
                     APRWLRSRPERRTLWLALLLVCMGCMWFFLSLDRGMGRWDKGIKWLLGVPCLLFIAAF
                     PPRPQAFFWGLPVGCIGMGSLAMWQAWAQGMERPEGYTNAIQWGNLALLLGCMAMVCA
                     AIFWRWRAWWWRAFMAVAVVAGVAASLLSQSRGGWLAVVLIFPALLTQVRQIRPRLFG
                     RLLALSVALLLALVVVLAVTPRFHERIGQAVEEITQYFNAGIAGTSLGVRLDQYRLVA
                     EMIPQKPWLGWGAHGYVDEMTRRVELGEFDKALVFYPQVHNDFLDIWVKVGVFGVLLQ
                     AALFAYVLFLFWPTRRRLQQWPESSSTWHDALALRVLGSLIPVAYLMFGMSQPFFNHN
                     SGIMFFVFYVSVLWAALRGVERGIWPPGEARSVP"
     gene            14260..15270
                     /locus_tag="PK518_07945"
     CDS             14260..15270
                     /locus_tag="PK518_07945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM48884.1"
                     /translation="MTWLSVLVPVYNVAPYLEECLASVVQQLPADGGVQLLVLDDCST
                     DGSWALMQQLDRRWPGRLQLLQHARNGGLSAARNTMIDAATGDYLWFLDSDDKLLPGA
                     IAGLQAIVRQHAPDVVLCDFAVWRERMRLKHRLRGEGHKRSFDGPARQLVHDRAQLMA
                     GMLGTGQLHAWSKISRRPLWGDDRSGVRFPAGRYFEDMATMPLMALRAQSFYYETSPW
                     VAYRQRDSSILASMSLRKALDQSAALLPLAQALAGQPEWQHAGLRLALAQQAARSLVG
                     AMRHVCQSPDGTAALAEQLRQNFRAASPLTPEELARAYLQRGWWLRRRRFLRWFHATP
                     TP"
     gene            complement(15367..>15564)
                     /locus_tag="PK518_07950"
     CDS             complement(15367..>15564)
                     /locus_tag="PK518_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRM48885.1"
                     /translation="HEAEIPAYPIQNALMGPVRRAAAQTGDAGHIALWAGQGVGAVRA
                     LTAGVLLQTLEREWQDARNLL"
BASE COUNT         2576 a         5157 c         5230 g         2601 t
ORIGIN      
        1 cggcgcgagc cggccgcgcc ggcatccgca cccgaagggg ccggcgagca gcgcccgccc
       61 cagccccagc cgcgtcgcac gcatccgctg ctcgagcagc tggccggctg gtacccgcat
      121 ctgttcggtg cgcagttcct gccgctcaag cgcggcatct tccacgacct gatggccgcg
      181 cacggcgagg ccatcgacaa ggacgcgctc aagctcgccc tcgccatcca cacccgctcc
      241 acgcgctacc tgaatgccgt ggcccagggc atgaagcgcc atgacctgca aggccaggcc
      301 gtggaggaca tggcgcccga gcatgtgcac cacgcgctgt tggaggtgtt ccgccgccgc
      361 aaaccgcgtg acggcgagga cttgacggcc acgctgcgcc ggcgcatcgc ccaggccttc
      421 gaggccagcg gcctcacccg cgaggcctac gacgccctgg tgcgcgggcg cgacgacaag
      481 gccaacgccc tgctggacga agcctttgcc gaagtggcgg agcgcgatgc caaggccgag
      541 gccctggcgc gcgccttcga ggccagcggc gccacgcccg agcagttcgc cgacatgtac
      601 ggcatggatc gccgcgccgt cacccagtcc ctggcgcggg cacgccgccc gcaggccaag
      661 ggctgaggag cggcgtcatg tggctgggcg tatcgctgtt ctacgtgggc gccgtgctgt
      721 gcctgaacgg cctgtggatg cgcggccata tcgccgaccg cgagatctgg gtcatcaacg
      781 tcttcgtcgg cctggtgtcg ctggcgatag gcctgttttc catcttcggc ccgggcgccg
      841 acgcccattc catccagggc ggggcgctga cgctgctgtt cgccttcacc tacctgtggg
      901 tggcggccaa ccgcttcagc ggcgccgacg ggcgcggcct gggctggttc agcctgttcg
      961 tcgcgattac cgccgtgccc gtggcgctgg acaccctggc cggcgcccgc agcctgctgg
     1021 actggtggat gggcgccaac tgggccgcct gggctctgct gtgggccctg ttctggctgc
     1081 tgctggcgcg cgggcgcgac atcggtcgct tcaccggcgc catgtgcatc gcccagggcg
     1141 tgctgacggg ctggctgccg ggctatctgc tgctttccgg tcggctttac tgatacagcc
     1201 cgttgatctt tctacaggtc gtggcagcgg cttcagccgc gaatggtgtt gttcgcgact
     1261 gaagccgctg ccacaacatc cgccttcaca ggcgtggctc cacgcctgat ttctgttggt
     1321 tgcgtggcct gtctgggttt tgaagcatgg ccaaaggccc ggtcggtgcg ctaggcggcg
     1381 gctgcaactt gtcgaaattt tttacatatc tcccggcacg gagaatgctt tctctggaaa
     1441 atccaatgaa atcaatggca ttttccagtg gcacaaacgt tgcttatgcg tttccgggtg
     1501 tcaacctttt gtcaacggca cctgaaagga ttcgatatga gcaaaaaact gggttccatg
     1561 gccgctgcga ttctgatgac gctcggcaca tccgcatacg ccggtgcgtt tgtcaatggt
     1621 ggctttgagg atggcaacct caatggctgg acgggcgggg gtggctcgtg gtttggtggc
     1681 ggcgcaccgg ttgatcctgc gacctacgca ggcggcccgt ccaacaacac catcatgatc
     1741 ggtggtaccg accccatcac cggggccaac cgggtctatg gcggcggtta ctcggtacgc
     1801 gtcaacgacg aagtgaacaa ccgttctgtc tcgaccataa gccagacggt caacaactac
     1861 accgacaaca gcatcttctt tgcctggaac gcggtgctgg aagactcaca tggcctcacg
     1921 gattccgact atttctctct tacgctgagg gacaaaacga cgggcaccaa cctggtgagc
     1981 cgcgcctaca gctctgccgg ctccattggg tcggggacgt ccggcgtgac ctggacgaag
     2041 tttggcaact ggtactcctc gggctgggtg gtggagcaga tcgacctggc ggcactgggt
     2101 gccgtggggc atgacttcac gctgacactg ctggcctctg actgccctta tagcggtcat
     2161 gcaggttacg tctatctgga tggctttggc gccgtgaatc cgcccaccgg ccaggtgccc
     2221 gagcccggct cgctggcact gctgggcctg ggctttgccg gcctggccgc ggcccgccag
     2281 cgccagaaga aagccgcctg atccgcaagg atcacccggc aggcgcagcg ccccggaaag
     2341 gggcggcgcc agcaacagaa ggccgcagaa ccgcggtctt tttccattct ggcgtgtcct
     2401 gttttcagcg ccgctgcagc gttgcccaca gggcggccag cacgatcagt gcgccgccgg
     2461 ccagggtgcg cggctgcagt tgggcggcgc ccagcagcac cgatgacacg ctggcgaaca
     2521 gcacctccga cagcatcacc acgccggtga tcgccgtggg caggcgcgcg gcgccgtact
     2581 gcagcgccca gttgcccagc atcagcagca gcgccagggc ggcggccatg gcggcccagc
     2641 gcgggtcgaa ggccggaaag gccggcacca cgcccgccgc cagcccggcc agcgcggcca
     2701 gccagcccag gcccatgcaa ccggcaaaca tggccagcat gcgcgccggg ccgggcacgg
     2761 tgtgcaggcg ccgcagcagc acgttggtca cggcaaaggt gaagccgccc agcagcgcca
     2821 gcgcgtcggc cagcgacagg ttggccagca ggccgcccca gctggccccg gccggcacca
     2881 gcaccagcgc cacgccgcca aaggccagcc ccaggcgtgc cagcgccgcc ggcgtggggc
     2941 gctcgcccag gatgcgccag gccagcagca ccagccaggc gggcatgagg tagaacacca
     3001 gcaccacgcg caccacgtcg cccacggtca cggcccagtt gaaggccatg ttgttgatgc
     3061 ccgagttcag cgccagcagc catagcgccg gctggcgcag cagatcgccc aggctgccgg
     3121 ggcgcgccgc cagcatcacg gcgcagacca gtgtgtacat gacggccgtg gcccacagcg
     3181 ggtgcaggcc ggcggcatgc atgctgcgaa acggccacca cgacaggccc cacagcaggg
     3241 cgttgcacag cagggccagg acgggcaggg gagattgcat gaaaagtgcg tgcttgtttc
     3301 tttggcccgg gagcgccggc gtctcgccgg cccgcaggtg gccaagtgtg gcggcccctc
     3361 catgccggcg ggacgccggc gctcccagag gcggagatga ggtgcttaga ttatcggtaa
     3421 aaatgcctgt agcgcctgca gataaagcgc agacagctat caaaatcagt gctgatccag
     3481 gcgtgcgatc ttgcgcagct gcaccatttc cttggggtag tggcgcacgt tgtagacatg
     3541 ccagcgcagt atcaccacca tcaccgcggc caccagcagg ccgaaggcgg cgatggcgcc
     3601 aaacgccgac agcccccatt tggtgaacag gctgtagagc acgcccatgc ccaggatggc
     3661 agcctgttca ttgaagtttt gcaccgcaat cgagcgtccc gcgcccatca ggttgtggcc
     3721 acggtgctgc aggagcgcgt tcatgggcac gaccaggaag ccgcccagcc cgccgagcag
     3781 gatcaagaag ggcacggcca gccagatgtt gtcgatggcg ttcatcaata tcaccaccag
     3841 acccatggcc acgcccaggg gcagcacgcg cgtggccttg tccagctgcg tgtacgacga
     3901 ggccagcacc gcgcccgcgg ccgtgcccag ggccaccacg cccaccaggg ccgaggcctg
     3961 ggtggtgctg tagcccaggg ccacgccggc ccaggccagc acgatgtagc gcagattgcc
     4021 cgagacgccc cagaacagcg tggtggtgga cagcgagatc tgccccagcc ggtcgcgcca
     4081 caggcggttg ttgcaggtcc agaagtcgcg tagcagcacc agcagctggc gcggcatggg
     4141 gcgcatggcc acgccggtca gcggtatgcg caggttgatg gcggccgcca gcacgtacac
     4201 cggcaccagg gcgaggatgg cggcctcggc cggcaggtcc agccccagat caatgcccgg
     4261 catgtccagg cccagcagca ggccggagac atgcggcgcc accagctggc cgccgagcac
     4321 cacgccaaga atgatggagc ctatggtcag cccctcgatc cagccattgg ctttgaccag
     4381 ctgcgacgcg ggcagcagct cggtgaggat gccgtacttg gccggcgagt acgccgccgc
     4441 gcccagcccc accacgccat aggccagcag cgggtgcgag ccgaacagca tcatcaggca
     4501 gcccaccacc ttgatggcat tgctgatgaa catcacccgc cccttgggca tggagtcggc
     4561 aaacgcgccc acgaagggcg ccaggaccac gtagaacagc gcgaacatcg gcaccagggc
     4621 ggcacgctgc cattcgggtg aaccgccgct gcgcagcagc tccaccgcgg tcacgaacaa
     4681 ggcgttgtcg gccagcgagc tgaaaaactg cgccgacatg atggtgtaga aaccgcgctt
     4741 catcggatgg ctggtgcttc ccgggccgct gggccgaggg actgtgatat ttggaaacac
     4801 tcctcggagc gacgggcggt tatatcacgc gcctggagcc cggcgatgcc agaatgcgcc
     4861 cgtcctgata cgtgttaaca cctccatgcc gcgccccatt ctcgccacca tccaccctgc
     4921 tgcggtgcag cacaacctgg agcgcgcgcg ccgcgcggcg cccgatgcgc gcgtgtgggc
     4981 ggtggtcaag gccaatgcct atggccacgg catagagcgg gtgttcgagg ccctgcgctc
     5041 ggccgacggc attgcgctcc tggatctggc cgaggcgcag cagctgcgcc agctcggctg
     5101 gcgcggcccc atcctgctgc tggagggcgt gttcgagccg cgcgacctgg agctgtgctc
     5161 gcgcctgtcg ctgtggcatg tggtgcattg cgagcagcag atcgactggt tggcggcgca
     5221 caagacccag gtgccgcagc gcgtgttctt gaagatgaac agcggcatga accgcctggg
     5281 cttcacgccc gggcgctacc gcagcgcctg ggcgcgcctg aacgcgctgc cgcaggtcga
     5341 tgaaatctcg ttcatgaccc atttcagcga cgccgacagc gcgcgcggca tagcgcacca
     5401 gctgcgggcc ttcgagcagg ccacgcagga cctgccgggc gagcgctgca tcgccaacag
     5461 cgccgccatc ctgcgccacg gtggcgcggc cgaggtgcgc ctggactggg tgcgcgccgg
     5521 catcgtgctc tacggcagcg cgcccgacca ccccgagcgc acggccgccg cctgggactt
     5581 gcagccggcc atgaccctgg cctcgcgcct cattgccgtg cagcagctgc aggccggcga
     5641 cagcgtgggc tatggctcgc gcttcactgc cgatgggcct ttgcgcatcg gcgtggtggc
     5701 ctgcggctat gccgacggct atccgcgcca tgcagacacc ggcacgccgg tgctggtggg
     5761 cggcgtgcgc acgcgcaccg tgggccgcgt cagcatggac atgatcaccg tcgatctgac
     5821 acccgttccc gatgcaggcc cgggcgcaga ggtcacgctc tggggccgcg ccggcaacgg
     5881 tgccctgctg cccatagacg aggtggcgca ggccgccggc accgtgggtt atgagctgat
     5941 gtgtgcgctg gcgccgcgcg tgccggtgac catggattag ggcctatacc cgacgccgcc
     6001 agtcaacatt aatttagatt gttctaatgt tgttctagat caagcaatga cctggggttt
     6061 ccactaagaa tggcttcgca gcgcaccgat cgctgcgtga tagccaacta aaaaattgtg
     6121 gagttgcccc atgtcctatc catccaggtc ttcctctctg cgacatgtcc tgcgcttcgc
     6181 actcggcgca gtgggcgccg ctgccctgct ggcggcggcc ccggcgcgcg ctcaggagcc
     6241 cgtgcagccg attgccccgg ccaaggtcac caacccggcc aaggtcgagc tgggcaagaa
     6301 gctgtggttc gacccgcgcc tgtcgcgctc gggcttcatc tcgtgcaact cctgccacaa
     6361 cctgagcatg ggcggctcgg acaacctcaa gacctcgatt ggcgacaagt ggcagaaggg
     6421 cccgatcaac tcgcccacgg tgctcaactc cagcctgaac ctggcgcagt tctgggacgg
     6481 ccgcgccaag gacctgaagg agcaggccgg cggcccgatc gccaacccgg gcgagatggc
     6541 cttctcgcat gacctggcgg tggacatgct gcgctccatc ccgcagtacg tgggcgagtt
     6601 caagcgcgtg tttggccacg acaagctggg catcgacgag gtcacggccg ccatcgccgc
     6661 cttcgaggaa accctggtca cgcccaacgc gccgtttgac aaatggctca agggcgacaa
     6721 gaaggccatc acggcgcagg agctgcgtgg ctaccagctg ttcaagggct cgggctgcgt
     6781 ggcctgccac aacggcccca acctgggcgg cacctcgttc cagaagatgg gcctggtcga
     6841 gccctacaag acggacaacc cggccgaggg ccgctccgcc gtgaccggca acgacgccga
     6901 gcgcttcaac ttcaaggtgc cgacgctgcg caacgtggag ctgacctacc cctacttcca
     6961 cgatggcgcc gccgacaccc tggccgaggc cgtggacacc atgggtcgca tccagctggg
     7021 gcgcaagttc agcgacaagg aaaacgccga catcgtggct ttcctgaaga cgctgaccgg
     7081 cgaccagccc cggatcgcca tgcccatcct gccgccgtcg agcgacaaga ccaagcggcc
     7141 ccagcccttt gagtgaatgt tcaacaattc atggtagctc gtgaaagtcg gcccgcctga
     7201 cgggccttta cgggccggca ctccgcgagg gggccggctt ctttttttgc ttttcaggcc
     7261 agtgcccgct gcacggcggg gcgcgcggcc atgcgttcgc ggtgtgccgc caccttggga
     7321 aagcgcgcaa tgtccacgcc gtcgcttttc agccagccgg ccatggtgaa caggtagccg
     7381 tcggccacgc tgtattgcgc gcccagcacc caggggccct ggtccgtgtg cgccaggtag
     7441 tggcgctcga tgatgtcaaa gcattcggtc atgttggccg gcaccttgcg ctgcatcgcc
     7501 gcctgggcct cgggctcgtc ggcccagcgg gcggcgcgcg ggcggtgggc atgggcgata
     7561 tgcacggtgg aggccaggta gctgttgaac tcctgcatgc gtgccaggcc gaaggcgtcg
     7621 gtgggcgcca gctgcgcggc cggaaagcgc tgcgccacat acaccagcag ggccggggtt
     7681 tcggtcagcg tgccctgctc ggtcaccagc gcgggcacgc ggcccttggg gttcacggcc
     7741 aggtactcgg gcgtgcgctg ctgctggctg gcaaagtcca cgcgcaccag tgcatggggc
     7801 gcctgtgcct cctccagtgc gatgcgcacc gcctgggcgc aggtgccggg ggcgtagtac
     7861 agggtcaatg caggcatttt gtgctccttt aaaaatagct gtttacgcaa tacagtaaag
     7921 cgttacagcc tattttgttt caaattctgg tctgaaacca cgcgccgggg cgggtgtggc
     7981 cgccgcgtcg attgtcctgg ttatagtccc caccccatgg ccaaggacaa aaccctctac
     8041 acctgcagcg aatgcgccag caccagcccg cgctggctgg gcaaatgccc cggctgcggc
     8101 gcctggaaca cactgatcga gaccgtgccc gaggcggcgc ccggcaagaa ccgcctgagc
     8161 aacgccgcgg gccagtacgc cggcctggcc caggcccagg cggtgatgcc cctggccgcc
     8221 atcgaggcca ccgaggtggc gcgcaccgcc agcggcgtcg aggagctcga ccgcgtgctc
     8281 ggcggcggcg tggtcgaggg cggcgtggtg ctgatcggcg gcgacccggg cataggcaag
     8341 tccacgctgc tgctgcaggc catggacgcg ctgcagcgcc ggggcctgcc cacgctgtac
     8401 gtgaccggcg aggagagcgg cgcccaggtg gcgctgcgct cgcgccgcct ggggctggat
     8461 gccagccagg tgaacgtgct ggccgagatc cagctggaga agatcctcgc caccatagag
     8521 gccacgcagc ctgccgtggc ggtgatcgac tcgatacaga ccgtttactc agaccagctc
     8581 agcagcgcgc ccggctccgt ggcccaggtg cgcgaatgcg cggcgcacct gacgcgcctg
     8641 gccaaggcca cgggcatcgc catcatcctg gtcggccatg tgaccaagga gggggctctc
     8701 gccggcccgc gcgtgctgga gcacatggtg gacacggtgc tgtacttcga gggcgacacg
     8761 cactcaagct ttcgcctggt gcgcgccatc aagaaccgtt ttggcgcggt gaacgagatc
     8821 ggcgtcttcg ccatgacgga gcgcggcctg aagggcgtgg ccaaccccag cgcgatattc
     8881 ctctcccagc atgccgagcc cgtgcccggc agctgcgtgc tggtcacgct ggagggcacg
     8941 cgccccatgc tggtggagat acaggccctg gtggatggcg gcgggcccag cccgcggcgc
     9001 ctgtcggtcg gcctggagcg cgaccgcctg gccatgctgc tggcggtttt gcaccgccac
     9061 gcgggcgtgg cctgcgcgga ccaggatgtg ttcgtcaacg ccgtgggcgg cgtgcgcatc
     9121 agcgagcccg cggccgacct ggcggtgatg ctggccatca ccagcagcct gcgcggccgg
     9181 gcactgccca agggcttcat cgccttcggc gaggtcggcc tggccggcga ggtacgcccc
     9241 gcgccgcgcg gccaggagcg gttgaaagag gcggccaagc tgggctttac cgtggccgtg
     9301 gtgcccaagg cgaatgcgcc caaaaagccc atccccggcc tgcagatcca ccccgtggag
     9361 cgtgtggacg aggccatgga tgcggtgcgc gggttggggt gaggcgagtg gccactgtgc
     9421 aagcctgcag ataggtgtat aagtgtctat gcgcataagc gccgaattgg agtcatcatg
     9481 agcacggtgc gctggaacat cgctgtatcg caggaagtgg atcaatccgt gcgcattttt
     9541 cttgccgccc agggtggggg gcgcaagggt gatttgtccc gcttcatcga agaatcggtg
     9601 cgcgcccacc tgctggagcg cgcggtcgat caggccaaga ccgcagccgc tggactgggc
     9661 gagagcgagc tgacggagct cattgacgag gcagtgcagt gggcgcgtga aggcggcggg
     9721 cgctgatgcg cgtcattctc gacaccaatg tgctgctggg cgcgctgatt tcaccgtatc
     9781 gtccccccga cttgatctac cgcgcctggc gcgcatcgcg tttcgagctg gtcacgtccc
     9841 tggagcagct cgacgagttg cggcgcgtca gccgctatcc caaactcaag gccatcctgc
     9901 ccgcccaccg cattggaacc atggtcaaca gcatgcagcg cgccctggtg ctgcccatgc
     9961 tgccgccatt gccggagggc gtgcaagcca ctgaccccaa cgataccttc ctgcttcaga
    10021 tggcagtggc gagcgcggcc gactacctgg tgaccggcga ccgcagggcc gggctgctgc
    10081 aacgtggcag tcttggccgc acacgcatcg tcacgccggt ggctttctgt gacgaggtgc
    10141 tttgacgcgt actacgcaag acccacagcc atgtactccc cggcgcatac gacaatgccg
    10201 cccatgaatc tgcgcaagtt cctcatcccg ctgggcatcg ttgtgctcgt catcgccgcc
    10261 tggcgcgcct atggcccgca gggcatcctc accgtgagcg gcggcctggt catgtggggg
    10321 ctgctgcact acacgcgcct catgaacgtg atgcagaagg cgcgccacaa ccccataggt
    10381 tacgtggcca gcgccgtgat gctgaatgcc aagctgaaac ccggcgtgaa tctgatgcat
    10441 gtgatggcca tgacgcgcgc tttgggcgag cagctctcgg ccgagggcca ggagcccgag
    10501 gtctaccgct ggacggatgg cacgaagtcg catgtgacct gcaagttttt caatggccgg
    10561 ctggccagct gggagctggt gcgccccgca ccgggcgagg aacccgccgc cgaatcgtaa
    10621 aatagccggc tttgcgactt tcgcagttct ctaaaggatc accatgagcc ccgttgttcc
    10681 ctcgatggcc gatcgtgacg gcaagatctg gatggacggc cagatggtgg actggcgcga
    10741 cgccaagatc cacgtgctga cccacacgct gcactacggc tgcggcgcct tcgagggcgt
    10801 gcgcgcctac aacaccgtga acggcacggc catcttccgc ctgcaagagc acaccgaccg
    10861 gctgttcaac agcgccaaga tcctgcgcat gcagctgcca ttcagcaagg acgaggtcaa
    10921 ccaggcgcag atcgacgtgg tcaaggccaa caatctggag agctgctacc tgcgcccgtt
    10981 gacctggata ggctcgcaaa agctcggcgt cagccccaag ggcaaccaga tccacctgat
    11041 ggtggccgcc tgggcctggg gcgcctacct gggcgaagag ggcatgcagc gcggcatccg
    11101 cgtcaagacc agcagctaca cgcgccacca cgtcaacatc accatgacgc aggccaaggc
    11161 ggtgagcaac tacagcaact ccatcctggc caacatggaa gccctggacg acggctacga
    11221 cgaggccctg ctgctcgacg ccgccggctt tgtgtcagaa ggcgcgggcg agaacatctt
    11281 cgtcgtcaag gacggcgtgg tctacacccc cgacctgtcc gccggcgccc tgaacggcat
    11341 cacgcgcaac accgtgctgc acatctgcaa ggacctgggc ctggacctcg tgcaaaagcg
    11401 catcacgcgc gacgaggtct acatcgccga cgaggccttc ttcaccggca cggcggccga
    11461 ggtcacgccg attcgcgagc tcgaccgcgt gcagatcggc gccggcagcc gcggcccgat
    11521 caccgagaaa attcagagcg ccttctttga catcgtcaac ggccgcaacc ccaaatacgc
    11581 ccactggctc accaaggtct gaacccccat gacacaagca atcgttgaac tgctggccaa
    11641 ggatctggac ggcaacggcg gagtgcactg ccccaacccc aaggccgaca tgaagctgtg
    11701 gaacagccac ccgcgcgtgt tcctcgagat cgcccaccag ggtcatgcct cgtgcccgta
    11761 ctgcggcacc gagtaccggc tcaaggcggg tgaggtgctc aagggcggac attgagcctg
    11821 ggcggctgga gagccgccgt agttggccag gtgtttctgc agcccatacc aatgaactgg
    11881 cgcggtctgt gaaggtgctg cactttgtga ccggaggctt ctccggtgcc acgcaggtgg
    11941 cggtcgatct gtgcctggcc gcccggggcg agccccatat gcagaccctg ctggtgctgc
    12001 ggcgcaagcg caatacctca gcagagcgcg tgcaggcgct gcgtgaccag gggctgcagg
    12061 tgcagctggt gtccaactgg ctgcatgcgc tcacggtatg ggagttacgc aaaatcattc
    12121 gcgactggcg tccggacgtg gtctttgcgc atggctttag cgaccatatc tggggccggc
    12181 gcgccgccgt ggccgagggc gtgccgcgca tcttccatgt cgagcacaac tcacgcgagc
    12241 gctacacgcc ccggcgcctg cgccaggcat tggagcttga gccgcacacc caggccagca
    12301 taggcgtgtc ggaaggggtg cgcaccgcac tcatcgagcg cggttttgcc ccgggcaaat
    12361 gcctggccat cccgaatggc attgcgctgg agcgctttcc tgcaagcttg ctaccgcagc
    12421 gctgggaaca gcgcgagccc gccatcctga tggcatcgcg ttttgcccgg caaaaggatc
    12481 acaccacgct gatagcggcg ctgggcctgc tgcgcgagcg tggcctgcgg cccacgctgt
    12541 acctggccgg cgccggcaag gccggcctgc gcggcaaggc cgaggccctg gcgcagtccc
    12601 tcggtttgca ggagcaggtg cgcttcctgg gcaatgtgcc tgacctgccg cagcgcctgg
    12661 ccgccaccca ggtgttcgtg ctgtcctccc actgggaggg gatgccattg gccctggtcg
    12721 aggccatggc cgcaggctgc gcctgcgtgg gctcggatgt cctgggtgtg cgcgaggtgc
    12781 tggagcatgg ccgcaccggc ttgctggtgc cgcctgccga ggcgactgcg ttggcatacg
    12841 cgttgcagca gttgctgcaa aaccccgccc aggcgcagcg cctggggcag gctgcacgcc
    12901 agcaggcgct tacagcttgt gggcgcgagc atatgtggcg gcgctatcgt gccttgctga
    12961 atgcccctgg cgggcgtgac tgatggacta caaggccgaa accccattca agctgacgac
    13021 gttggccacc tttctgctgg cggcgctggc gatctgggtg ccttcgggct actcctatgg
    13081 tgcggtactg ttgttggccg gcgccttgtt ctacgcgcca cgttggctgc gcagccggcc
    13141 ggagcgccgc acgctatggc tggcattgct gctggtctgc atgggctgca tgtggttttt
    13201 cctgtcgctg gatcgcggca tgggccgctg ggacaagggc atcaagtggc tgttgggcgt
    13261 gccgtgtctg ctcttcattg ccgcctttcc gcccaggccg caggccttct tttggggctt
    13321 gcccgtcggc tgtatcggca tggggagttt ggccatgtgg caggcctggg cgcagggcat
    13381 ggagcgtccc gagggctaca ccaacgccat tcagtggggc aatctggcct tgctgctggg
    13441 ctgcatggcc atggtctgcg cggcgatatt ctggcgctgg cgtgcctggt ggtggcgcgc
    13501 attcatggcg gtggccgtcg tggccggggt cgcagcgtcg ctgttgtcgc agtcgcgcgg
    13561 cggctggctg gcggtggtat tgatattccc cgccctgctg acccaggtca ggcagattcg
    13621 cccaaggttg tttggccgct tgctggcact gtcggtggcc ctgctgctgg cattggtggt
    13681 ggtgctggcc gtcactcctc ggtttcatga gcgcattggc caggcggtag aggaaatcac
    13741 ccagtacttc aatgccggca tcgccggcac ctcgctgggc gtgcgcctgg atcaataccg
    13801 gctggtggcc gaaatgattc cgcaaaaacc ctggctgggc tggggcgcgc atggctatgt
    13861 ggacgaaatg acccggcgtg tggagctggg tgaattcgac aaggcgctgg ttttctaccc
    13921 gcaggtgcac aacgattttc tggatatctg ggtcaaggtc ggcgtgttcg gggtcttgct
    13981 gcaggcggcc ctgtttgcct atgtgctgtt cctgttctgg cccacgcggc ggcggttgca
    14041 gcagtggccg gagagttcgt ccacctggca cgatgccctg gcattgcgtg tcctgggcag
    14101 cctgatcccg gtggcctatc tgatgttcgg catgtcacag ccgttcttca accataacag
    14161 cggcatcatg ttctttgtgt tctatgtgag cgtgctgtgg gcggcattgc gtggcgtgga
    14221 gcggggtatc tggccaccgg gcgaggcaag gagcgtaccg tgacctggct gagcgtgctg
    14281 gtgccggtgt acaacgtggc gccctatctg gaggaatgcc tggcctcggt ggtgcagcag
    14341 ttgccggccg atggcggggt gcagctgctg gtgctcgacg actgctccac agacggttca
    14401 tgggccttga tgcagcaact ggatcggcgc tggccggggc gcttgcagct gctgcagcat
    14461 gcgcgcaacg gcggcctgag cgccgcgcgc aacaccatga tcgatgccgc aaccggcgac
    14521 tacctgtggt ttctggactc ggacgacaag ctgctgccgg gtgccattgc cggcctgcaa
    14581 gccatcgtgc gccagcatgc accggatgtg gtgctgtgcg actttgccgt ctggcgcgag
    14641 cgcatgcgcc tcaagcaccg cctgcgcggc gagggccaca agcgcagctt tgacggcccg
    14701 gcgcggcagc tggtgcacga ccgggctcag ctcatggcgg gcatgctggg cacggggcag
    14761 ctgcatgcct ggtccaagat ctcgcgccgc ccgctgtggg gcgatgaccg gagcggtgtg
    14821 cgctttcccg ccggccgtta tttcgaggac atggccacca tgccgctgat ggcgctgcgg
    14881 gcgcagagtt tttattacga aaccagcccc tgggtggcct accgtcagcg tgacagcagc
    14941 attctggcga gcatgagtct gcgcaaggcg ctggaccagt cggccgcgct gctgccgctg
    15001 gcgcaggcgc tagcgggcca gcccgagtgg cagcacgccg gcctgcgcct ggcgctggcg
    15061 cagcaggcgg cgcgcagcct ggttggcgcc atgcgccatg tctgccagag cccggacggc
    15121 acggccgcgc tggccgagca gctgcggcag aactttcgcg ccgcctcgcc gctcaccccc
    15181 gaggagctgg cgcgagccta tttgcagcgc ggttggtggc tgcgccggcg caggttcctg
    15241 cgctggtttc acgccacgcc aacgccgtga accatggcgc tgtagccggt gtggcacgga
    15301 aagcggtttg gtattaaaaa aatcgccttg tagcgcccat atgacaagcg ctaatagcta
    15361 ttaaaattat agtaaattgc gggcatcctg ccactcccgc tccagcgtct gcagcaaaac
    15421 gccggccgtc agcgcccgca ccgcccccac cccttgtcca gcccacaacg caatatgccc
    15481 agcatcaccc gtctgcgccg cggcgcggcg caccgggccc atcagggcgt tctgtatcgg
    15541 ataggcgggg atctcggcct cgtg
//
LOCUS       DAOMTQ010000063        15492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_598_length_15492_cov_26.236121, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000063 DAOMTQ010000000
VERSION     DAOMTQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15492
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_15492_cov_26.236121"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..669)
                     /locus_tag="PK518_07955"
     CDS             complement(310..669)
                     /locus_tag="PK518_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRM48886.1"
                     /translation="MRFSVNTTKRQVPACAALAFFVVAAMPLSLSAHQTVDVIIQNYR
                     FEPAEVRIRSGDTVRWTNQEKRTSHSVLFSAEQGGESERLFPQDVWQRRFEAPGRYPY
                     SCGPHPEMKGVVEVVAP"
     gene            complement(693..1520)
                     /locus_tag="PK518_07960"
     CDS             complement(693..1520)
                     /locus_tag="PK518_07960"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRM48887.1"
                     /translation="MKKSTLSMMLLGSLLAVGTQAAFAAPDWGKVPKRDVNVFHPAVS
                     PMEWVGKKSDHSGTAGLKKGETCVGCHEEKGTLNFDMKRLAGKDMEPKGAPKTMMYPV
                     AVQAAYDASNLYIRLTFKAPGGGADQSDKDNEVKATMMFPDAQVQLANQAGCWASCHN
                     DARTMPGADDKKTKYTKSGAYELMQWKSAKGAKVANGTVTTERKMEGGSLGATAEGGK
                     SGDTYTVTFTRKNPGEGKTVPFGLAIHADHSTGRFHHVSLGYTLGVGADGDVKAVKQ"
     gene            complement(1585..2178)
                     /locus_tag="PK518_07965"
     CDS             complement(1585..2178)
                     /locus_tag="PK518_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRM48888.1"
                     /translation="MAGKLASLWAKLKKPSAKYSLIGLLATGFISGILFWGGFNTGME
                     ATNTMDFCISCHEMHDNVYQEYKKTIHYSNRTGVRAMCSDCHVPKDWTHKMIRKIQAS
                     REVWGKLTGTIDTPEKFEAKRLVLAEREWARMKAADSRECRNCHSFEGMDTDKQKARG
                     AKMHKIAQDEKQTCIDCHKGIAHNKPKGMKEDEDEDL"
     gene            complement(2272..3894)
                     /locus_tag="PK518_07970"
     CDS             complement(2272..3894)
                     /locus_tag="PK518_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRM48889.1"
                     /translation="MAQDAKIEAPAMTAAEKEAAKKIYFERCAGCHGVLRKGATGKNL
                     EPHWSKVEKDGKKTEGGTLALGTGRLERIIGYGTDGGMVNFDDILTKEELNLMARYIQ
                     NTPDVPPEFSLKDQLDSWNVIVPVDKRPTKQMNKINLSNVFSVTLRDTGEVALIDGDT
                     KEIKSIVKTGYAVHISRLSASGRYVYVIGRDGRVSLIDLWMETPAVVAEVKIAFDARS
                     IDTSKFKGFEDKYAIAGGYWPPQYTIMEGDTLKPLKVVSTRGVTVDGDYHPEPRVASI
                     VSSFIKPEWVVNIKETGQILLVDYTDIENLKTTTIGSAKFLHDGGWDASKRYFLVAAN
                     ASNKIAAVDTKKGTLAALVDVAKIPHPGRGANFTHPKYGPVWATGHLGADVVTLISTP
                     SENAKHKQFKQYNWKVVQEVKHVPGNLFVKTHPVSKHLWADSAQNPEKDLAESVTVWD
                     LADLSKPKAVLHVAKDAGLPETKAVKRAVHPEYSKDGKEVWISLWGGKTDQSAIVVYD
                     DATLKVKKVITDPKMITPTGKFNVYNTQHDIY"
     gene            4230..4862
                     /gene="ccmA"
                     /locus_tag="PK518_07975"
     CDS             4230..4862
                     /gene="ccmA"
                     /locus_tag="PK518_07975"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HRM48890.1"
                     /translation="MKSGSILAFTDLGCSKGGRQLFKGVHCTVPAGRWLHVAGANGIG
                     KTSLLRMLCGLSPIESGDILWNGQSIRMRRGSFQRDLSYLGHLNGLQESMTVQENLAF
                     ATALRGHAPDVKAVAATLARFGVGGRGGQLVRHLSQGQKRRVALARLSLSPARLWVLD
                     EPFVAMDDSGISMLSDLIAGHLAQGGITVLTSHQRVDIGQVPAQILELRA"
     gene            4859..5566
                     /gene="ccmB"
                     /locus_tag="PK518_07980"
     CDS             4859..5566
                     /gene="ccmB"
                     /locus_tag="PK518_07980"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRM48891.1"
                     /translation="MKPPPSPVPKPLGLGAIMLAVLRREIALAMRQKGEVLTPLVFFI
                     VIASLFPLGVGPESALLLRMAPGVLWVSALLAAMLSLQRMFASDYADGSLEQMLLSST
                     PLALLVAAKALSHFLLSGLPLVLVAPVLGLQFGLDGPALGILMLSLLLGTPTLSLIGS
                     IGASLTLGVRGAGVLLSLLILPLYIPVLIFGAGAVEAYASGLGPGGHLSLLAALLVLA
                     LFFAPLATTAALKISLE"
     gene            5563..6303
                     /gene="ccmC"
                     /locus_tag="PK518_07985"
     CDS             5563..6303
                     /gene="ccmC"
                     /locus_tag="PK518_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HRM48892.1"
                     /translation="MKTPGIQWFKYASPQNFYFLAGKLWPWFAGLAAVLTLVGLWIAF
                     FVAPTDAQQGQGYRIIFVHVPTSQMSMFIYIVMAGWAAIGLAFNTRLSGMMASALAPT
                     GALFAFLSLVTGSLWGKPMWGTWWVWDARLTSELILLFLYLGFLALQSSIDDPRRADK
                     ACAVLALVGVVNVPIIYFSVVWWNTLHQGASVSMTRAPSMAAPMLWGMLIMVVAFWMY
                     AVAAALARVRNIILERERHTEWVRHAVE"
     gene            6323..6472
                     /gene="ccmD"
                     /locus_tag="PK518_07990"
     CDS             6323..6472
                     /gene="ccmD"
                     /locus_tag="PK518_07990"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HRM48893.1"
                     /translation="MGGYAFYVWGSFGATALVMAVESLLIRGRRKQMQQSLRAELHHP
                     TELHT"
     gene            6469..6906
                     /gene="ccmE"
                     /locus_tag="PK518_07995"
     CDS             6469..6906
                     /gene="ccmE"
                     /locus_tag="PK518_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRM48894.1"
                     /translation="MKPRHKRAAIIAGGLAAISLAAFLVLNAFESNLVFFFSPSQVAA
                     GEAPKNRTFRVGGMVKEGSIDRKDLTVSFIVTDTAQEMKVSYTGILPDLFREGKGVVA
                     QGKLSEDGLFTASEVLAKHDENYMPPEAQHAVDEAQKTIKTLQ"
     gene            6909..8876
                     /locus_tag="PK518_08000"
     CDS             6909..8876
                     /locus_tag="PK518_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRM48895.1"
                     /translation="MIPELGHFSLILSLFVALVLGLLSLIGAHQGRRDWVVLARPATQ
                     ALWLLTALAYGCLTYAFYSNDFSVVYVVQHSNSQLPDIYRIAGVWGGHEGSLLLWHFM
                     LTSWMLAVSLFSRQLPDAMVARVLGVLGLVAVGFLVFMLFTSNPFLRLADAPADGRDL
                     NPLLQDPGLVFHPPMLYMGYVGLAIPFAFAIAALLNGRLDAAWARWSRPWATAAWIFL
                     TLGIALGSWWAYYELGWGGWWFWDPVENASFLPWLVSAALIHSLAVTEKRGLFRNWTI
                     VLAITAFSLSLLGTFLVRSGVLTSVHAFATDPRRGVFILIFLCVVVGGSLTLFAMRAG
                     KVRSGGTFTLMSRETFLLFNNVLLTTAAASVLLGTLYPLIVDALNLGKLSVGPPYFNA
                     VFVPIMVPVLLFMVVGTAARWKSDDVMDLARKLGISALAALVLGCSLPLLAGPWSAGA
                     ALGLSLALWIVLSSLQQIYRQLRGTANWRATPWSFWGMHMAHIGIAVSVIGVTMVTSY
                     ETEKDVRMGLGDTVAVGGYTFRLTGIREAEGPNYRADVGDVQLMRDGQLLRTLHPEKR
                     TYFSSAMPMTEAAIDTGLTRDVYVSLGERLEGGEHPAWAVRVYYKPFVSWIWGGGLLM
                     ALGGTLAALDRRYRSKRVRATAGAGIGGATA"
     gene            8873..9400
                     /locus_tag="PK518_08005"
     CDS             8873..9400
                     /locus_tag="PK518_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HRM48896.1"
                     /translation="MKKKALIPLALFLVLAVFLAIGLTRDPRVVPSPLIGKDAPQFTA
                     PVLASPEKTFALQDMRGKVWLLNVWASWCTACLQEHPVLVDYARRKDIPLVGLAYKDK
                     PADSIKWLQRKGDPYDFTVVDQDGRIGIDFGVYGVPETFLIDQAGKIRFKQIGPVTVE
                     ALEKQILPLIRELQK"
     gene            9397..9876
                     /locus_tag="PK518_08010"
     CDS             9397..9876
                     /locus_tag="PK518_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRM48897.1"
                     /translation="MRLWLALLLALQCALAGLAAQANEAQPLAEDPVVEKRMVAISAE
                     LRCLVCQNESLAGSRADLALDLKREIRSLIKAGKSDAEIMDFMVSRYGDFVRYRPPVN
                     PVTWLLWFGPFVLLAGAAYGLVRMVRSNQRASATTPTLDEAQRAKAQSLLQDTDKLP"
     gene            9873..10733
                     /gene="ccmI"
                     /locus_tag="PK518_08015"
     CDS             9873..10733
                     /gene="ccmI"
                     /locus_tag="PK518_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRM48898.1"
                     /translation="MTIFIAIIAALTLLAVLALVRPLLRPRSYESVSSQRLNATIYKD
                     QLAALDRDLASGAINPADHETTRDELQLRLLDDTAAPDAPAGNPGTGFWSGRRTAALI
                     AVLLPLLSVGTYRWIGDPAALTPGATQEAARLQITQMVESLEAKLKAEPNNPQGWAML
                     ARSYKVMGRLDEAEQAYEKATDAIRTHPDLLVDFAELLAMKAQHRMDGRPEELVRQAL
                     AIDPEHPSALMMSGVVAYQRSDFDGAVRDWEKLLALLEPGSEDAKMTQENLDDARAQS
                     ALTKAQKNRP"
     gene            10809..13079
                     /locus_tag="PK518_08020"
     CDS             10809..13079
                     /locus_tag="PK518_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HRM48899.1"
                     /translation="MNTHCPDHPAPRTLPLLVSLACLGMLGAAQAQPGPQAHATLPTV
                     VVSSTQNNLLADDLPLSADVIEGQNLSDQQTRTLRQALQDLPNTSVRSSPTRLAVSAG
                     SAAFARDGNMGINIRGLGGNRVLMTVDGIRMPRSYVSRAAMFDREYLSLELFKRVDVI
                     RGPASAQYGGDGMAGVVNFVTHDPMDFLKTEEGQAPRTLGGRVAAGWSEDDHGYYAAG
                     TVAAKASDSLQWMLTATGRQSHALDNMGSNDAPNSTRTRPDPTDNRDASVLGKVVLRP
                     SKLQRHVLTFEHTRKTSDIDLLSSRAAGAPARPTDVLDEYADTRIERDRLTWDARFGV
                     NTDWADHLRTVVAMQDSSSRRVGNSFLRNGVHRVRDNRYKEHSWQLGLQADKVLRHGD
                     WSHRIVYGLDHVRSKITNLYDGQAPLPPESFPLKRFPDTRQTSSGLYLQDETVYGDWT
                     ITPGLRIDHFAIDVTSQNLYYPPAPQPGKSLSGSAALPKLGVLYRATPAWSIFGQYAT
                     GYRAPEAGQVNDRFEAVALGVTNIIIANPDLKPERSRGVELGVRGRFDRLSLDLVGFA
                     NRYSNLIEDARLIQKTATQQTFQAVNIARARIHGFELKGGYDWGSVGPGRLHNTFAYG
                     QARGTDKTTDQPLNSITPMQIALGLRYDTAPWSVYADLRHYAAKKDKDIDLVSIGNNK
                     AGTTQFATPSATTLDLGAQWRITKAVRVNLALNNLTNRKYWIWPDVYGQPAASTTIDA
                     YTQPGRNARVSLVADF"
     gene            complement(13116..13808)
                     /gene="kdpE"
                     /locus_tag="PK518_08025"
     CDS             complement(13116..13808)
                     /gene="kdpE"
                     /locus_tag="PK518_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HRM48900.1"
                     /translation="MNEPRILIVEDEANIRRFVRLTLAAEGYEVFEAASLERGLIEAG
                     TRRPDMLVLDLGLPDGDGIDLIRDLRAWSQAPVIVLSARSSEEDKIAALDAGADDYLV
                     KPFGAGELLARVRAQLRRWQDRTADGAPQIAFGDVVVDLVKRSVTHAGDALHLTPIEY
                     RLLVHLAQHGGRVLTHLQLLQAIWGPGHSEDSHYVRVHMANLRKKIEADPSRPRHLLT
                     EAGVGYRFVMSE"
     gene            complement(13805..>14271)
                     /locus_tag="PK518_08030"
     CDS             complement(13805..>14271)
                     /locus_tag="PK518_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM48901.1"
                     /translation="ACAPSLQGRDVAVRLAPEVPLLHLDAALFECVLVNLLENAVKYT
                     PPATPLQIAAEPQGEAVRITVDDEGPGLPPGREEAVFEKFERGSKEAAAPGVGLAICR
                     AIVQAHGGRMHGENRIVDGRVAGARFVITLPRGEPPQDDGSQAAVLPDNAMP"
     assembly_gap    14272..14371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14372..>15492)
                     /locus_tag="PK518_08035"
     CDS             complement(<14372..>15492)
                     /locus_tag="PK518_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRM48902.1"
                     /translation="APGSRHPKRWQDVEELLAAGIDVWSTMNVQHLESLNDVVGGITG
                     VRVWETVPDTLFDAAEEEVVVDLPPDELLARLAAGKVYVPEQAQQAAANFFRKGNLIA
                     LRELALRRTADRVDTQMQDWRRAAQGGALGQPVWATRDALLACIGPGEGGEAVVRSTA
                     RLATQSNLPWHAVFVDAPAQQRVDPAARQQALRALKQAQDLGAMTATPTGIGVAQALV
                     RYAREHNLSRLVLGRRQGRRRWRLSMTERLADLTPDMDVLQVPLPPKAHGRQRPADAR
                     AKKPYWPGYLAATGACALVALLAGALHGVLELTNIVMLFLLSVVGVALLFGRGPATLA
                     ALLGVAMFDFFFVPPRMSFNVSDVQYLITFAVMLVVALV"
BASE COUNT         2570 a         5219 c         4759 g         2844 t
ORIGIN      
        1 caccgcgtac gcccttctgc tcctgcgccg aggcggccag tgcaatcgtc gatagcagga
       61 ctgccgcgac acgcgtgagt cgatggcttt ttatgtaatg tctccacgga tgatgttgtg
      121 tgatcggccc ctgacaaggc caaggtctcg cgtgccgtca tcgccggcca ccagaccctt
      181 gccaatatag gaaggccagg acatcgcagg ctgacctgca tcaacccgga aaaacaactg
      241 tgcgtcctga agacttttgc cgcctgcgcc tgtagcggcc agaggcagta tgcaaaaact
      301 gcgctgtggc tacggcgcca ccacctccac cacacccttc atttccgggt gcggcccgca
      361 ggaatatgga taccgtccgg gtgcctcgaa tcggcgctgc cagacatcct gggggaagag
      421 ccgttcggac tcaccaccct gctcggcaga aaacagcacc gaatggctgg tgcgcttttc
      481 ctgattcgtc caacgcaccg tatccccgga gcgaatccga acctctgccg gctcaaaacg
      541 gtagttctga ataatcacat ccacagtctg gtgcgcagac agggacaaag gcatggcggc
      601 aacgacaaaa aaggcaagcg ccgcgcatgc cggcacctgc cttttcgttg tgttgaccga
      661 gaagcgcatg ttctcaagcc atggggcgtt gcttactgct tgacagcctt cacgtcgccg
      721 tcagcgccca cgcccagggt gtagcccagg gagacatggt ggaagcgtcc ggtggagtgg
      781 tcggcgtgga tggccaggcc gaacggcacc gtcttgccct cgccggggtt cttgcgcgtg
      841 aaggtgacgg tgtaggtgtc accgctcttg ccgccctcgg ccgtcgcgcc cagcgagccg
      901 ccttccatct tgcgctccgt ggtcacggtg ccgttggcca ccttggcgcc cttggcgctc
      961 ttccactgca tcagctcata ggcaccgctc ttggtgtact tggtcttctt gtcgtctgcg
     1021 ccaggcatgg tgcgcgcatc gttgtggcag gaagcccagc agccggcctg gttggccagc
     1081 tgcacctgcg catccgggaa catcatggtg gccttgacct cgttgtcctt gtcgctctgg
     1141 tcggcaccgc cgccgggcgc cttgaaggtc agacggatgt agaggttgct ggcgtcatag
     1201 gcggcctgca cggccaccgg atacatcatg gtcttggggg cgcccttggg ctccatgtcc
     1261 ttgccagcca gacgcttcat gtcgaagttc agcgtgccct tttcctcgtg gcagccaacg
     1321 caggtttcgc ccttcttcag gccggccgtg ccgctgtggt cagacttctt gcccacccac
     1381 tccattggcg aaaccgcagg gtggaacacg ttgacatcgc gcttgggcac cttgccccag
     1441 tcaggtgcgg caaaggctgc ctgtgtaccc acagccagca gcgaacccaa aagcatcatc
     1501 gaaagggtag attttttcat cggtcactcc tagtagatgt tctgcaggcg gccgcccccc
     1561 accggaactg gccgccatcc aagcttacag atcttcgtcc tcgtcttcct tcatgccctt
     1621 gggcttgtta tgcgcaatcc ccttatggca gtcgatgcag gtctgctttt catcctgcgc
     1681 gatcttgtgc atcttggccc cgcgtgcctt ctgcttgtcg gtgtccatgc cctcgaagct
     1741 gtggcagttg cggcattcgc gcgagtcggc cgccttcatg cgcgcccatt ctcgttcggc
     1801 caagaccagg cgtttcgcct caaacttctc cggcgtgtcg atggttccgg tgagcttgcc
     1861 ccacacctcg cggctggcct ggatcttgcg gatcatcttg tgcgtccagt ccttgggcac
     1921 atgacagtcc gaacacatgg cacgcacgcc ggtgcggttg ctgtagtgaa tggtcttttt
     1981 gtattcctga tagacgttgt catgcatctc atggcaagag atacagaaat ccatcgtgtt
     2041 ggtcgcctcc atcccggtgt tgaaaccacc ccagaacaga atgccagaga taaaacccgt
     2101 cgccaacaac ccgatcagcg agtatttggc actcggtttt ttcagtttcg cccacaggct
     2161 tgcaagtttt ccagccatca aactctccgc actcgattat ttttgcattg gagacgcgta
     2221 aatcgagggg ctttcgcccc tcgattgttg cctcagatca aggcttctgc atcaatagat
     2281 gtcgtgctgc gtgttgtaca cgttgaactt gccggtgggc gtgatcatct tgggatcggt
     2341 gatcaccttc ttcaccttca gagtcgcatc gtcgtacacc acgatggccg actggtcggt
     2401 cttgccgccc cacagggaga tccagacttc cttgccatcc ttggagtact cggggtgcac
     2461 ggcgcgcttg accgccttgg tctccggcag gccggcgtcc ttggcaacat gcagaaccgc
     2521 cttcggcttg gacaggtcgg ccagatccca caccgtcacg gattcggcca gatccttttc
     2581 agggttctgc gccgagtcag cccacaggtg cttggacacg ggatgggtct tcacgaacag
     2641 gttacccggc acatgcttga cttcctgcac aaccttccag ttgtactgct tgaactgctt
     2701 gtgcttggca ttctccgaag gcgtggagat cagcgtcacg acatctgcgc ccaggtgacc
     2761 ggtagcccag acggggccgt acttcgggtg cgtgaagttg gcgccgcgac ccgggtgggg
     2821 aatcttggcc acatcgacca gggcagccag cgtgcccttc ttggtgtcca ccgcggcaat
     2881 cttgttcgat gcattggcag ccaccaggaa gtagcgcttg ctcgcatccc aaccaccgtc
     2941 atgcaggaac ttggcagaac caatggtggt cgtcttcagg ttctcgatgt ccgtgtagtc
     3001 caccagcagg atctggccgg tttccttgat gttcaccacc cattccggct tgatgaacga
     3061 ggacacgatg gacgccacac gcggttccgg gtggtagtcg ccgtcaaccg tcacgccgcg
     3121 ggtggagacg accttcagcg gcttcagggt gtcgccttcc atgatggtgt actgaggcgg
     3181 ccagtagcca ccggcgattg catacttgtc ctcgaagccc ttgaacttgg aggtatcgat
     3241 ggagcgtgca tcaaaggcga tcttgacctc ggccacgacc gccggcgttt ccatccacag
     3301 gtcgatcagg gacacacggc catcacggcc gatcacataa acgtagcgcc cggaagccga
     3361 cagacgcgag atatgcaccg cgtagccggt cttgacgatg ctcttgattt ccttggtgtc
     3421 cccgtcaatc agagccacct caccggtgtc gcgcagcgtg accgagaaga cattgctcag
     3481 gttgatcttg ttcatctgct tggtcggacg cttgtcgacc ggcacgatga cgttccagct
     3541 gtcaagctga tccttcaggc tgaactcggg cggcacgtcc ggagtgttct ggatataccg
     3601 tgccatcagg ttcagctctt ccttggtcag gatgtcgtcg aagttgacca tgccgccatc
     3661 ggttccgtag ccgatgatcc gctccaggcg acccgtgccc agtgcgagag tgccgccctc
     3721 ggtcttcttg ccgtcctttt cgaccttgga ccaatgcggc tcaaggttct tgcccgtagc
     3781 ccccttgcgc agcacgccgt ggcagccggc gcaacgctcg aagtagatct tcttggccgc
     3841 ctctttctca gcggccgtca tggcaggcgc ttcgatcttg gcgtcttgag ccattgcagg
     3901 tactgcaagg ccggagagca tcactgcagc tgcgatgctc gacaggcggg gaaatttcat
     3961 cttctttact ccattggttt cttgcagcca atccaccctc tcaggcagtt ttgaaacccg
     4021 gcgacggcca caacaagtcg atccgagtaa gcccattatg tttgtgcagt tacttaaatc
     4081 cttgacctaa gccaaaagag catgtggtat gcatcaaaac gttgacgaat gtgcacacaa
     4141 ttcaggcatt gccaaaactt ggcgatatgc gcatcagtca tgcgcagcca ttcacgtaag
     4201 atgcgtgctt gtgacgtgga aacaacaaat tgaaatcagg ttcgattctt gcctttactg
     4261 acctcggttg cagcaagggg gggcggcagc tgttcaaggg cgtccattgc acagttccgg
     4321 cagggcgctg gctgcatgtg gcaggagcca atggcatagg caagaccagc ttgctgcgca
     4381 tgctgtgcgg gctgtccccc attgaatcgg gcgacatcct ctggaacggg cagtccatac
     4441 gcatgcgacg cggcagcttt caacgcgatc tgagctacct ggggcatctc aacggcctgc
     4501 aggaatcaat gaccgtgcag gaaaacctgg cattcgccac cgccttgcgc gggcacgccc
     4561 ccgacgtcaa ggcagtcgct gcgacgctcg cgcgcttcgg tgtcggcgga cgtggcgggc
     4621 aactggtgcg ccatctgtca caggggcaaa agcgccgcgt agcgctggcg cgactgtccc
     4681 tgagcccggc acgcctgtgg gtgctcgacg agccctttgt ggccatggat gactcgggca
     4741 tcagcatgct ctccgacctg atagccgggc acctggcgca gggcggcatc accgtgctga
     4801 ccagccacca aagggtcgac ataggccagg tgccggccca gatcctggag ttgcgcgcat
     4861 gaagcccccc ccttcccctg tccccaagcc gttggggttg ggcgccatca tgctggcggt
     4921 actgcgccgc gaaattgccc tggccatgcg acaaaagggc gaggtgctga cgccgctggt
     4981 gtttttcatc gtcattgcca gcctgtttcc gctgggcgtg gggccagaat cggcgctgtt
     5041 gctgcgcatg gcgccgggcg tgctgtgggt cagcgcgctg ctggcagcca tgctgtcgct
     5101 gcagcgcatg tttgccagcg actacgccga tggttcgctg gagcaaatgc tgctgtcgtc
     5161 caccccgctg gccctgctgg tcgcggccaa ggcactgtcg cattttttgc tgtccggctt
     5221 gccgctggtg ctggtggcac cggtattggg actgcagttt gggctggacg gcccggcact
     5281 gggcattctg atgctgtccc tgctgctggg cactcccaca ctgagcctga ttggcagcat
     5341 aggagcgtcg ctgaccttgg gtgtgcgcgg cgcaggggta ctgttgtcgt tgctaatatt
     5401 gccgctctac atccccgtgc tgatctttgg tgcaggtgcc gtggaggcct acgcctccgg
     5461 cctgggacct ggcggacacc tgtccctgct tgcggcgctg ctggtgctcg cgctgttctt
     5521 tgcccccctc gccaccacgg ccgccctgaa aatttccctc gaatgaaaac acctggcatt
     5581 caatggttca agtacgcctc gccgcagaac ttttactttc tggccggcaa gctctggccc
     5641 tggttcgcgg gcctggccgc cgtactgacc ctggtcggcc tgtggatcgc cttctttgtc
     5701 gcacccaccg atgcacagca gggacagggc tatcgcatca tcttcgtgca tgtgccgaca
     5761 tcgcagatgt cgatgttcat ctacatcgtc atggccggct gggcggccat cggcctggcg
     5821 ttcaatacgc gcctgtcggg catgatggcc agcgccctgg cgcccacggg tgccctgttc
     5881 gcattcctgt cgctggtaac gggctcgctg tggggcaagc cgatgtgggg tacctggtgg
     5941 gtatgggatg cacgactcac gtcggaactg atcctgctgt tcctgtacct gggttttctg
     6001 gcgctgcagt ccagcatcga cgacccgcgc cgtgccgaca aggcctgcgc cgtgctggcg
     6061 ctggtaggcg tggtgaacgt gcccatcatc tatttctctg tcgtctggtg gaacactttg
     6121 caccagggcg catcagtatc catgacccgc gcgccctcga tggcggcccc catgctctgg
     6181 ggcatgctga tcatggtggt ggccttctgg atgtacgcag tcgccgccgc cctggcgcgg
     6241 gtgcgcaaca tcattcttga gcgggaacga cataccgagt gggtacgaca tgcagtggaa
     6301 tagtgtttcg gaattttttg ccatgggcgg ctacgccttt tatgtctggg gcagcttcgg
     6361 cgccacggcc ctggtgatgg ccgtggagtc actgctgatc cgcggccggc gcaagcagat
     6421 gcagcaaagc ctgcgcgccg aacttcatca ccccaccgag ttgcacacat gaaaccccgt
     6481 cacaaacgcg ccgccatcat tgccggtggc ctggccgcca tctcgctggc tgctttcctg
     6541 gtgctcaacg cctttgaaag caacctggtg ttcttcttca gccccagcca ggtggccgcg
     6601 ggcgaggcgc ccaagaaccg caccttccgc gtgggcggca tggtcaagga gggttcgatt
     6661 gatcgcaagg acttgaccgt ttccttcatc gtcaccgaca cggcccagga aatgaaggtc
     6721 agctacaccg gcatccttcc ggatctgttc cgcgagggca agggcgtggt ggcacagggc
     6781 aagctgagtg aggacggcct gttcacggct tccgaggtac tggccaagca tgatgagaac
     6841 tacatgccgc ccgaggcaca gcacgcggtt gacgaggccc agaagaccat caagacgctg
     6901 cagtaaccat gattccagaa ctagggcatt tctcgctcat cctttccctt ttcgtggcgc
     6961 tggtgctggg gctgttgagc ctcatcggcg ctcaccaggg gcgccgtgac tgggttgtgc
     7021 tggcacggcc cgcaacgcag gcgctgtggc tgctcacggc ccttgcctat ggctgcctga
     7081 cctacgcctt ctacagcaac gacttctcgg tcgtgtatgt ggtgcagcat tccaactccc
     7141 aactgccgga catctaccgc atcgccggcg tctggggcgg gcatgagggt tcgctgctgc
     7201 tgtggcattt catgctgacc agctggatgc tggcggtgtc gctgttctcg cgccagctgc
     7261 ccgatgccat ggtggcgcgg gtgctgggtg tgctcggcct ggtggcggtg ggcttcctgg
     7321 tgttcatgct gttcacctcc aaccccttcc tgcggctggc cgacgcgccc gccgacgggc
     7381 gtgacctgaa cccgctgctg caagacccgg gcctggtgtt ccacccgccc atgctctaca
     7441 tgggttacgt cggcctggcc attcccttcg cctttgccat cgccgcgctg ctcaatggcc
     7501 gtctggacgc cgcctgggcg cgctggtcgc gcccctgggc cacggcggcc tggattttcc
     7561 tcacgctggg catagccctg ggttcctggt gggcctacta cgagctgggc tggggcggct
     7621 ggtggttctg ggatccggtg gagaacgcct ccttcctgcc ctggctggtg agtgcggcgc
     7681 tgatccactc cctggcggtg acggaaaagc gcggcctgtt tcgcaactgg accatcgtgc
     7741 tggcgatcac ggccttctcg ttgagcctgc tgggcacctt cctggtgcgt tcgggggtgc
     7801 tgacctcggt gcatgccttc gccacggatc cgcgacgcgg cgtgttcatc ctgatcttcc
     7861 tgtgcgtcgt ggtgggcggc tcgctcacgc tgtttgccat gcgcgcgggc aaggtgcgct
     7921 cgggaggcac gttcacgctg atgtcgcgcg agaccttttt gctgttcaac aacgtgctgc
     7981 tgaccaccgc cgcggcatcg gtgctgctgg gcacgctgta tccgctgata gtggacgccc
     8041 tcaacctggg caagctctcc gtgggcccgc cctatttcaa cgcagtcttc gtgcccatca
     8101 tggtgccggt gctgctgttc atggtggtgg gcaccgctgc gcgctggaag agcgacgacg
     8161 tcatggatct ggcacgcaaa cttggcatca gcgccctggc ggctctggtg ctgggctgca
     8221 gcctgccatt gttggccggc ccctggtcgg ccggggcagc gctgggactg agcctggcac
     8281 tgtggatcgt tctgtcctcg ctgcagcaga tctaccggca gttgcgcggc acggccaact
     8341 ggcgcgccac gccctggtcc ttctggggca tgcacatggc gcatatcggc atcgccgtca
     8401 gcgtgattgg cgtgaccatg gtcacgagct acgaaaccga gaaagacgtg cgcatgggcc
     8461 tgggcgacac cgtcgccgtg ggcggctaca ccttcaggct cacgggcatc cgcgaggccg
     8521 agggcccgaa ctaccgcgcc gatgtcggcg atgtacagct gatgcgtgac ggccagctgc
     8581 tgcgcacgct gcaccccgaa aagcgcacct atttctcctc tgccatgccc atgaccgaag
     8641 cggccattga caccggcctg acgcgcgacg tctatgtatc gctgggcgag cgcctggagg
     8701 gcggcgagca ccccgcctgg gccgtgcgcg tgtactacaa gccctttgtc agctggatct
     8761 ggggcggcgg tctgctgatg gccctgggcg gcacgctggc ggcgctggat cgccgttacc
     8821 gcagcaagcg cgtccgcgcc accgcgggcg ccggcatcgg aggagccacg gcatgaagaa
     8881 aaaggctctc atcccgctgg ccctgtttct ggtgctggcg gtgtttctgg cgattggcct
     8941 gacacgcgac ccccgggtcg tgccctcgcc actgatcggc aaggacgcac cccagttcac
     9001 ggcccccgtc ctggccagcc ccgagaaaac ctttgccctg caggacatgc gcggcaaggt
     9061 ctggctgctc aacgtctggg cgtcctggtg cacggcctgc ttgcaggagc acccggtgct
     9121 ggtggactac gccaggcgca aggacatccc cctcgtcggc ctggcctata aggacaagcc
     9181 ggccgacagc atcaagtggc tgcagcgcaa gggtgacccc tacgacttca cggttgtaga
     9241 ccaggacggc cgcatcggca tcgacttcgg cgtctacggc gtgcccgaga cctttctgat
     9301 cgaccaggcc ggcaagatcc gcttcaagca gatcggcccg gtgaccgtgg aggctctgga
     9361 gaagcaaatc ctgcccctga tccgggagct gcagaaatga gactctggct cgccctgctg
     9421 ctggcgctgc aatgcgccct tgccggcctt gccgcgcagg ccaacgaagc ccagcccctg
     9481 gccgaagacc cggtggtgga aaagcgcatg gtcgccatct ccgcggagct gcgctgcctg
     9541 gtgtgccaga acgaatcgct ggccggctcg cgcgccgacc tggcgctgga cttgaagcgc
     9601 gaaatccgca gcctgatcaa ggccggcaag agcgatgccg agatcatgga cttcatggtc
     9661 agccgctacg gcgacttcgt gcgctaccgc ccgcccgtca accccgtcac ctggctgctg
     9721 tggtttggcc ccttcgtgct gctggcaggc gctgcctacg gcctggtgcg catggtgcgc
     9781 agcaaccagc gcgccagcgc caccacaccc acactcgatg aggcccagcg cgccaaggcg
     9841 cagtctcttc tccaggacac cgacaagctc ccatgaccat cttcattgcc atcattgcgg
     9901 ccctgaccct gctggccgtg ctggcgctgg tacgcccctt gctgcggccc aggtcatacg
     9961 aaagtgtttc cagccagcgc ctgaacgcca ccatctacaa ggatcagctc gccgcgctcg
    10021 accgcgacct ggccagcggc gccatcaacc ccgccgacca tgagaccacg cgcgacgagc
    10081 tgcaattgcg tttgctcgac gacaccgccg cgcccgacgc acccgccggc aaccctggca
    10141 caggcttctg gagcgggcgc cgcacggcgg ccctgatcgc cgtcctgctg ccactgctct
    10201 ccgtgggcac ctaccgctgg atcggtgacc cggcggcact cacccctggc gcgacgcagg
    10261 aggccgcgcg gctgcagatc acgcaaatgg tcgagtcgct cgaagccaag ctcaaggccg
    10321 agcccaacaa cccgcagggc tgggccatgc tggcgcgctc gtacaaggtc atgggccgcc
    10381 tggacgaggc cgagcaggcc tacgagaagg ccaccgacgc cattcgcacc caccccgacc
    10441 tgctggtgga ctttgccgaa ctgctggcca tgaaggccca gcaccgcatg gacggccgtc
    10501 ccgaagagct ggtgcgtcag gcgctggcca tagaccccga gcatcccagc gcgctgatga
    10561 tgtccggcgt cgtggcctac cagcgcagcg acttcgacgg cgccgtccgg gactgggaaa
    10621 agctcctggc cttgctggaa cccggctcgg aggacgcgaa gatgacccag gagaacctgg
    10681 acgacgcgcg cgcccagagc gccttgacca aggcacaaaa gaaccgcccc tgagcccggc
    10741 acaccaaagt ctttcaaccc ggcccacgga acgccagcca ccccgcatcc ccccatagtc
    10801 accagcccat gaacacccac tgccccgacc accctgcccc gcgcaccctg ccccttcttg
    10861 tgtcgctcgc ctgcctgggc atgctgggcg ctgcgcaggc gcagccaggg ccgcaggccc
    10921 atgccacgct gccgacggtg gtggtcagca gcacccagaa caacctgctg gccgacgacc
    10981 tgccgctgag cgccgacgtc atcgagggcc agaacctgag cgaccagcag acccgcaccc
    11041 tgcgccaggc gctgcaggat ctgcccaaca cctcggtgcg cagctcgccc acgcgcctgg
    11101 ccgtcagtgc cggctcggcg gcatttgcgc gcgacggcaa catgggcatc aacatccgcg
    11161 gcctgggcgg caaccgggtg ttgatgacgg tggacggcat acgcatgccg cgcagctatg
    11221 tgtcgcgcgc cgccatgttc gaccgcgaat acctgtcgct ggagctgttc aagcgcgtgg
    11281 acgtgatccg cggcccggcc tcggcacagt acggtggcga tggcatggcc ggcgtggtga
    11341 acttcgtcac gcacgacccg atggactttc tcaagaccga ggaaggccag gcgcccagga
    11401 ccctgggcgg gcgcgtggcc gcaggctgga gcgaggacga ccatggctac tacgccgccg
    11461 gcaccgttgc cgccaaggcc agcgacagcc tgcaatggat gctgacggcc acgggccgcc
    11521 agtcccacgc actggacaac atgggcagca acgacgcgcc caatagcacc cgcacccgcc
    11581 cagaccccac ggacaaccgc gacgcatcgg tgctcggcaa ggtggtgctg cgcccgagca
    11641 agctgcagcg ccatgtcctc accttcgagc acacgcgcaa gacatcggac atcgacctgc
    11701 tctccagccg cgccgccggc gcgccggcca ggcccaccga tgtcctcgat gagtacgccg
    11761 acacacgcat cgagcgcgac cggctgacct gggacgcgcg ctttggcgtc aacaccgact
    11821 gggccgacca cctgcgcacg gtggtggcca tgcaggactc cagctcccgc cgagtgggta
    11881 acagcttttt gcgcaacggt gtacaccggg tgcgcgacaa ccgctacaaa gagcatagct
    11941 ggcaactggg tcttcaggcc gacaaggtgc tgcgccacgg cgactggtca caccgcatcg
    12001 tctatggcct ggaccatgtg cgcagcaaaa tcaccaacct gtacgacggc caggccccgc
    12061 tgccgcccga gagctttccg ctcaagcgct ttcccgacac gcgccagacc agcagcgggc
    12121 tgtacctgca ggacgagacg gtgtatggcg actggaccat cacccccggc ctgcgcatag
    12181 accacttcgc catcgacgtg accagccaga acctgtacta cccgcctgcc ccccagcccg
    12241 gcaagtcgct gtcgggctcc gcagccctgc ccaagctggg cgtgctgtac cgtgccactc
    12301 ccgcatggag catttttggc cagtacgcca cgggctaccg cgcgcccgag gcgggccagg
    12361 tgaatgaccg cttcgaggcc gtggccctgg gcgtgaccaa catcatcatc gccaaccccg
    12421 acctcaagcc cgagcgcagc cgcggggttg agctgggcgt gcgcgggcgc ttcgaccgcc
    12481 tgagcctgga tctggtcggc tttgccaacc gctactccaa cctcatcgaa gacgcccgcc
    12541 tgatccagaa aaccgccacg cagcagacct tccaggccgt gaacatcgcg cgcgcgcgca
    12601 tccacggctt cgagctcaag ggcggctacg actggggcag cgtcggcccc gggcgactgc
    12661 acaacacctt cgcctatggc caggcacggg gtaccgacaa gaccaccgac cagccgctga
    12721 actccatcac cccgatgcag attgccctgg gcctgcgcta cgacaccgcg ccctggagcg
    12781 tgtacgccga cctgcgccac tacgcggcca agaaggacaa ggacatcgac ctggtctcca
    12841 tcggcaacaa caaggccggc accacccaat ttgccacgcc atctgccacc acgctcgacc
    12901 tgggtgcgca atggcgcatc accaaggccg tgcgcgtcaa cctggcactg aacaacctca
    12961 ccaaccgcaa atactggatc tggcccgacg tctatggcca gccggccgcg tccaccacca
    13021 tcgacgccta cacccagcca gggcgcaacg cgcgcgtatc gctggtggcc gatttctgac
    13081 gtcgctcccg ggcattggcg cccaattgac ccgggctact cggacatgac aaagcggtag
    13141 cccacgccgg cttcggtcag caggtggcgc gggcgcgacg ggtcggcctc gatcttctta
    13201 cgcagattgg ccatgtgcac gcgcacatag tggctgtcct cgctgtggcc cgggccccag
    13261 atggcctgca gcagttgcaa atgcgtcagc acgcggccgc catgctgcgc cagatgcacc
    13321 agcaggcggt attcgatggg tgtcaggtgc agtgcgtcgc cggcgtgggt gacgctgcgc
    13381 ttgaccagat ccaccaccac gtcgccaaac gcaatctgcg gcgcgccatc ggcggtgcga
    13441 tcctgccagc ggcgcagctg cgcgcgcacg cgcgccagca gctcgccggc gccaaagggc
    13501 ttgaccaggt agtcgtcggc cccggcgtcc agggcagcga tcttgtcctc ttcgctgctg
    13561 cgcgccgaca gcacgatcac cggcgcctgt gaccaggcgc gcaggtcgcg gatcaggtcg
    13621 atgccgtcgc catcgggcag gcccaggtcc agcaccagca tgtcggggcg gcgcgtgccg
    13681 gcctcgatca ggccacgctc aaggctggcg gcctcgaaca cctcgtagcc ctcggcggcc
    13741 agggtcaggc gcacgaagcg gcggatattg gcctcgtcct cgacgatcag gatgcgtggt
    13801 tcgttcaagg catcgcattg tcgggcagca ctgccgcctg gctgccatcg tcctgcggcg
    13861 gctcgccgcg cggcaaggtg atgacgaagc gcgcgcccgc cacgcgccca tcgacgatgc
    13921 ggttctcgcc atgcatgcgg ccgccgtggg cctgcacgat ggcgcggcag atcgccaggc
    13981 ccacgccggg cgcggccgcc tccttgctgc cgcgctcaaa cttttcaaac accgcctcct
    14041 ccctgccggg cggcaggccg gggccctcgt cgtccacggt gatgcgcacg gcctcgccct
    14101 gcggctcggc ggcgatctgc agcggcgtgg ccggcggcgt gtacttgacg gcgttttcca
    14161 gcaggttcac cagcacgcac tcgaacaggg ccgcgtccag gtgcagcagc ggcacctcgg
    14221 gggccaggcg caccgccacg tcgcggccct gcaaactcgg cgcgcaggcc gnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natcaccagc gccaccacca gcatcacggc
    14401 aaaggtgatg aggtattgca cgtcgctgac gttgaaggac atgcgcggcg gcacgaagaa
    14461 aaagtcgaac atggccacgc ccagcagcgc cgccagcgtg gccggcccgc gtccaaagag
    14521 cagcgccacg cccaccaccg acagcaggaa cagcatgacg atgttggtca gctccagcac
    14581 gccgtgcagc gccccggcca gcagcgccac cagggcgcag gcaccggtgg cggcgaggta
    14641 acccggccag tacggcttct tggcccgcgc gtcggcaggg cgctggcgcc catgcgcttt
    14701 ggggggcagc ggcacctgca acacatccat gtccggggtc aggtcggcca gacgctcggt
    14761 catcgacagg cgccagcgcc tgcgcccctg gcgccgcccc agcaccaggc gcgagaggtt
    14821 gtgctcgcgc gcatagcgca ccagcgcctg cgccacgcct atgcccgtcg gcgtggccgt
    14881 catggcgccc aggtcttgcg cctgcttgag cgcgcgcagc gcctgctggc gcgccgccgg
    14941 atcgacccgc tgctgcgccg gcgcatcgac gaacaccgca tgccagggca gattggactg
    15001 cgtggccagg cgcgccgtgc tgcgcaccac cgcctcgccg ccttcacccg ggccgatgca
    15061 ggccagcagc gcatcgcgcg tggcccatac cggctggccc agcgcgccgc cctgggccgc
    15121 gcggcgccag tcctgcatct gcgtgtccac ccggtcggcc gtgcggcgca gcgccagctc
    15181 gcgcagcgcg atcaggttgc ccttgcgaaa gaaattggcc gccgcctgct gcgcctgctc
    15241 cggcacatag accttgcccg cggccagccg cgccagcagc tcatcgggcg gcagatccac
    15301 caccacctcc tcctcggcgg cgtcgaacag ggtgtcgggc accgtctccc agacgcgcac
    15361 gccggtgatg ccgccaacca cgtcgttcag gctctccagg tgctgcacgt tcatcgtgct
    15421 ccagacgtcg atgccggcgg ccagcagctc ctccacatcc tgccagcgct tggggtggcg
    15481 cgagcccggg gc
//
LOCUS       DAOMTQ010000064        15172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_614_length_15172_cov_27.591983, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000064 DAOMTQ010000000
VERSION     DAOMTQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_614_length_15172_cov_27.591983"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PK518_08040"
     CDS             complement(<1..678)
                     /locus_tag="PK518_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM48903.1"
                     /translation="MALNWVWTGFFIVAFATAVVRWLLGEVGIFQALLTAMFDGARAG
                     FEISLGLAGIMALWLGLMRVGERAGMVELLARLAGPLLRRLFPAVPDGHPAQGAMTLN
                     ISANLLGLDNAATPLGLKAMQELQTLNREPSGTASNAQIMFVVMNTAGLTLIPTSVIA
                     IRQSVALKQGLGAGFNAADIFLPTLLVTFASLLAGVLAVAVAQRLPLWRARLWLPLLA
                     VGGLLAAA"
     gene            734..1378
                     /locus_tag="PK518_08045"
     CDS             734..1378
                     /locus_tag="PK518_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM48904.1"
                     /translation="MSQATLLVADDHPLFRAAIIGLLRERFAPFQTLEAASAATLGQA
                     LSEHPDVELVLLDLSMPGAHGFSALLHVRGEYPQVPVVVISSNDHPRVIRRAQQFGAA
                     GFIPKSAAAEDMALAIQTVLDGGSWFPAMAAERSEADAALAARLAQLTPQQFRVLLCL
                     ADGLLNKQIAHELGLAENTVKVHVTAILKKLDCYSRTQAAVLVKGLEPEQGTTP"
     gene            complement(1542..4211)
                     /locus_tag="PK518_08050"
     CDS             complement(1542..4211)
                     /locus_tag="PK518_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HRM48905.1"
                     /translation="MNQRRQLLWTVLVVLAGMALCMLLVGQIVQRRALNEESQDVQRR
                     LSLYAQALVQRIDRFRTLPEVLALDSELRTALAQPLTPAAVQALNHKLEQANGASQSS
                     TLTLIARDGLALAASNWRDAHSNVGENYHFRPYVQQALQHGQGRFYGIGLTTGEPGYF
                     LSTAIRDAQGRALGLVAVKIVFTELEREWRNGPDIVLASDTHGVVFLASRDAWRYRLL
                     NPLTAQDRGELAATRQYADQPLAPLLYRSERPVAGGGVVVRLQDPAEHRPLLWQTLEL
                     PGSDWRLHLLHDTRAVASAGRWAAVAAGGLWLALALLVLILRQRRRLALLRQRSRQEL
                     ETVLQQHAQELRTAQGAIVQAARQADTGLSRSLQHLPQGVVVVDKELNLVAWNSRYVE
                     LFRFPAELVRVGAPIEALLRYNARRGLLGPPPADDAVQRRLRHLREGTPYLHESSKSD
                     GQVLEIRGNPLPEGGFVTSYADITSYKNAARELRSLADALELRIAERTRDLDAARREA
                     VEANRYKTRFVAAAVHDLLQPLNAARMFSSLLRGHALDEAGRQVADSIDGALAAQDGI
                     LASLLDISRMESGQLEVRLRDFALGPLLQVVQHNFGIIAKSRGLRLDCVGSRYTVRSD
                     EALLRRILQNLLSNAIRYTPGGKVLIGCRRHGDQLRIEVHDQGPGIPESLQREIFEEF
                     RRLGEGHDDDRGAGLGLAIVERLGRLLGHEIGLRSELGRGSMFWVSVPIVATVGAAPS
                     LAPTESEPAQDQPLQGTTAWYVDDDAPSCAATGALLQRWGCDVPLAGGPQAALDLAAP
                     GNAPRLVLLDVRMGAFHGPDLYGDLCARWQQHPLVVLVTAERDTALRRQAAERGWGFL
                     LKPVRPSALRALISQMLLRQSEAEPSDTGKLQN"
     gene            4358..5680
                     /locus_tag="PK518_08055"
     CDS             4358..5680
                     /locus_tag="PK518_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRM48906.1"
                     /translation="MHAIPLQDTAAPERLPFYRQLYFQVVLAIVLGVLLGHFEPQYGA
                     QFKPLGDAFIKLVKMVIAPVIFLTIVTGIAGMTHLRTVGRVFGKAMAYFLCFSTLALI
                     VGMVVANVLQPGAGMHIRVADLDTKAVDSFVAKSHDLSLVGFAMDIIPKTLVSPFVGD
                     NILQVLFVAVLFGVALAMAGDAGKPALNFFEALTKPVFKLVNIVMKAAPIGAFGAMAF
                     TIGKFGLGSLVNLAELVATFYLTSLVFVLIILGAVARLCGFSVVKLMRYLKAELLLVL
                     GTSSSESALPSLMEKMEKAGCSKSVVGLVVPTGYSFNLDGTNIYMTLAALFIAQATDI
                     DLSLGQQLALLLVAMLSSKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFMS
                     ECRSLTNFVGNAVATVVVSRWENGLDYQRLHAALDGEAAAPMKETLAG"
     gene            complement(5737..6705)
                     /locus_tag="PK518_08060"
     CDS             complement(5737..6705)
                     /locus_tag="PK518_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HRM48907.1"
                     /translation="MFKNLIVYRIAPGWQADLTQLEEALAKTPFQECGATQEKAVGWV
                     PPRGDAHGPLAESVGGQWMLRFMAESKMLPATVLARKVKEKAALIEQQTGRKPGKKEA
                     KELKDEARLDLLPMAFTKQGSTWVWLDREAGLLMLDTGAQGRADEITSLLVEQLPGLS
                     LALLDTQTSPQAAMAHWLKSQEPPAGFSIDRECELKSAGEDKAVVRYARHPLDIEEVQ
                     AHIEAGKLPTKLAMSWDDRVSFVLTEGLQLKKIAFQDTVFEGQKQDDAGFDADVAIAT
                     GELSKLIPDLILALGGEGRGEIGGAATPVNGALHAPTSGPDDDAPF"
     gene            6903..7460
                     /locus_tag="PK518_08065"
     CDS             6903..7460
                     /locus_tag="PK518_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRM48908.1"
                     /translation="MTRFRITQRLAATALVAALAGIAAPSFAQPAPGAGPVAEQAQRP
                     RAMTPEQRQQFMKERMGQRLEAFKQKLQLTPAQEPAWNGFVQSMQPNAEPRHARLDWQ
                     GMDKLTTPERIDRMRAMHAQRGAEMERRGEAVKAFYAQLTPAQQKTFDDQGARMVGQR
                     GAKQGWGMHRHGHHHGFGPGASMGQ"
     gene            7697..8188
                     /locus_tag="PK518_08070"
     CDS             7697..8188
                     /locus_tag="PK518_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRM48909.1"
                     /translation="MTDLSHAPSIYLQGAIAEHDTEALTLGLTLPWREWRMPLWGGEL
                     RGHWDLYLSRWFFKGAARHGGTLVLGVTPTLRLRPDGGSSAWFWEAGIGATLADQRYH
                     PAGQEEFSTRFNFASHLGIGINFGARRQHELLLRLQHVSNAGIKKPNPGVEFLQLRYA
                     WHF"
     gene            complement(8410..11874)
                     /locus_tag="PK518_08075"
     CDS             complement(8410..11874)
                     /locus_tag="PK518_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM48910.1"
                     /translation="MTSAFWDRDLQSDTMRWNDGMERLFGVPLASLPPDGSSWTLRLH
                     PDDAPQVQQSLRQVIAGTAEFWCAQYRFRRQDGSYAWVDDRGFVLRDASGRGLRMVGG
                     MIDISLHWQAGQQYRSLFTEHPQPMWVCDQGSLRLLAVNPAMARHYGYDEQELLGMDM
                     RLLWPQAQRAQADSALAMCSDARAAHTLATAATTWRHARKDGTLMDMEVFVGVTDFDG
                     HPAWQILAHDITERSRIDAELARLSRAQRMRSACSETLLRANTEAELLHAVCAVAVQV
                     GGYRMGWVGLARDDERKRIEPVAHAGGSPEYLAQLRLSWSPDEPGGQGPASICVRSGR
                     TVVLRDMRANPTFDKVAGLLDALEFHSAIYLPLRGAERNLGLLCLYAPEVLEPGAQET
                     QLLEAMAADLAFGLENLRTRAEQQRLQAAIVKVAKAVSAGTGVAFFDHLAQHMADALS
                     AQVACVVRLQPPVDGQSLQAVTLSHISDGVVQPTLEYALSGTPSAQLLTQPLVVIEDR
                     VAQLFPHDPVLARCRVRSYVGRQLTGSDGTPLGMILVMYRKPLRRAHLVDTALQIFAA
                     RAAAELQRQADDMRIRQQASLLNKASDAIIVRDLEHRITFWNEGAERLYGWSRAEALG
                     HNAASLLYQDAQCFHQASAQVLRNGEWTGELQQYARDGRAIEVEGRWTLVRSEQGEAE
                     AILAINSNIGQRKASEREIQRLALFDALTGLPNRAHFMQRMGQALAAAQRQRQGGALL
                     FIDLDNFKQLNDTLGHDQGDLLLKSVAQRLNTCVRAMDTVARLGGDEFVVLLEQIGAQ
                     PATLAEHANKVGEKILNALSLPYALAGHQYRSTPSIGVALFDDQSTTVGELLKQADLA
                     MYQAKMAGRSTLRFFNPGMQKAVDEHVAFEADLRSALAQQEFSLHYQPQLNARGQILG
                     VEALLRWTQPRRGMVSPAEFIPAAEETGLILPLGRWVLHTACTRLAAWQKQPDLAPLT
                     MAVNVSPRQFRDAGFVADVLRVLAVTGAPAAQLKLELTESLLVEDMAKTIATMEALRA
                     HGVSFSLDDFGTGYSSLTYLKRMPLGQLKIDQGFVHDLLSDPNDAAIVRTIVALADSL
                     GLEVIAEGVETEEQRHWLAQAGCQAYQGYLFSKPLPAQEVEQLMQSHISAKNAAGTGA
                     DGLLAYSI"
     gene            complement(12300..13406)
                     /locus_tag="PK518_08080"
     CDS             complement(12300..13406)
                     /locus_tag="PK518_08080"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM48911.1"
                     /translation="MTLIDRPIPTPSTHDTTRIDDLRIKAVRPLITPAILEEWLPAPD
                     AAQELVEASRLQLSRVLHGQDDRLIVVVGPCSIHDHDQAMEYARLLKQEADALAQDLL
                     VVMRVYFEKPRTTVGWKGYINDPHLDGSFAINEGLERARLLLLDVLALGLPVGTEFLD
                     LLSPQYISDLVSWAAIGARTTESQSHRQLASGLSCPVGFKNGTDGGVKVAVDAIQAAQ
                     AEHAFMGMTKMGQAAIFETRGNHDCHVILRGGKAPNYSAADVQSTCELLQKSGQQAQV
                     MIDLSHANSSKQHARQIAVAEDVAAQIAGGDARITGVMIESHLEEGRQDIVPGQALRH
                     GVSVTDACISFAQTVPVLRQLAAAVRARRERNTP"
     gene            complement(13443..13916)
                     /locus_tag="PK518_08085"
     CDS             complement(13443..13916)
                     /locus_tag="PK518_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRM48912.1"
                     /translation="MRKLVPPVLLLAAGLLLAGCGTAPAARSSAYARLSAEQSSDIAI
                     HALGLVGTPYRFGGNTPEGGFDCSGLIGYVYNSRAGVAPPRTVALLSGFGASVPEDEL
                     RTGDLVLFGGGRPTHAGIYVGEGRFVHAPSSGGTVRLDHLRSRYWAQQSASYRRP"
     gene            14126..>15172
                     /locus_tag="PK518_08090"
     CDS             14126..>15172
                     /locus_tag="PK518_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM48913.1"
                     /translation="MTSLFVEGSNARDIATSLEHAMRSGALPPGSALPPVRQLAVELG
                     VNPNTVAAAYARLRAAGRVLTAGRKGTRVAGAPPSPTPVYAAPEGLRDLAGGQVDAAL
                     LPRLRGQAWTQALAQPLDGVAGDAPLLAQAAHWLQGQGLPGEDLGCFSGALDAIERAL
                     RQHARPGDRVGVEDPLWPPLLALLHSLRLVPVALAVDAQGMCVPADGVLRDCAAVVLT
                     PRAQHPTGVAMTAARWRALRRALRACPHTLLILDDHWGPLSAAPLAMAGRLPPLWLHV
                     LSVSSSLGPDCRVAVVSGTPGLVQGMCAHQALGPRAVSRWLQGLVAQLWRQASRGRGR
                     GSWQHMAGSYAARRV"
BASE COUNT         2276 a         5142 c         5224 g         2530 t
ORIGIN      
        1 ggccgccgcc agcaggccgc cgacggccag cagcggcagc cacaggcgtg cgcgccacag
       61 cggcaggcgc tgggccacgg ccacggccag cacgccggcc agcagtgagg caaaggtgac
      121 cagcagcgtg ggcagaaaga tgtcggccgc gttgaagccc gcgcccaggc cctgcttcag
      181 cgccacgctc tggcgtatgg cgatgaccga ggtcgggatc aaggtgagcc cggcggtgtt
      241 catgaccacg aacatgatct gggcgttgct ggcagtgccc gagggctcgc ggttcagcgt
      301 ctgcagctcc tgcatggcct tcaggcccag gggcgtggcg gcgttgtcca ggcccagcag
      361 gttggcgctg atgttcagcg tcatcgcgcc ctgggccggg tggccgtcgg gcacggcggg
      421 gaacaggcgg cgcaatagtg ggccggccag gcgcgccagc agctcgacca tgccggcgcg
      481 ctcgcccacc cgcatcaggc ccagccacag ggccatgatg ccggccaggc cgagcgagat
      541 ctcgaagccg gcgcgcgcac cgtcgaacat cgccgtcagc agcgcctgga agatgccgac
      601 ctcacccagc agccagcgca ccacggcggt ggcaaaggcc acgatgaaga aacccgtcca
      661 gacccagttc aatgccatgg agcttgatgg tagtgcaagg gtcatggcag gcggccgtgg
      721 cagactgcgg cccatgtccc aagccaccct gctggtcgcc gacgaccacc cgctgttccg
      781 cgcggccatc atcgggctgc tgcgcgagcg ttttgcgccg ttccagacgc tggaggcggc
      841 cagcgccgcc acgctgggcc aggccctgag cgagcatccg gacgtagagc tggtgctgct
      901 ggacctgagc atgccgggtg cgcatggttt ctcggccctg ctgcatgtgc gcggcgagta
      961 tccgcaggtg ccggtggtgg tgatctcgtc caatgaccac ccgcgcgtga tccggcgcgc
     1021 ccagcagttt ggcgccgccg gcttcatacc gaagtcggcc gcggccgagg acatggcgct
     1081 ggcgatacag acggtgctgg acggcggcag ctggttcccg gccatggcgg ccgaacgctc
     1141 cgaggccgac gccgccctgg ccgcgcgcct ggcgcagctg acgccgcagc agtttcgcgt
     1201 gctgctgtgc ctggccgatg gcctgctgaa caagcagata gcgcacgagc tgggcctggc
     1261 cgagaacacc gtcaaggtgc atgtcacggc gatattgaaa aagctcgact gctacagccg
     1321 cacgcaggcg gcggtgctgg tcaaaggcct ggagccggaa caaggaacaa ccccctgagc
     1381 agctgcgccg cttccccctt ccctctacgc gctgcgcgct acgggaaggg ggactttgct
     1441 cagtgcggcg gggcggccct tgcacggcgg ccctggcttt ggtcgcgcca gtatccaaca
     1501 aaatatggct ttacctaatg ttggtaaagc gctggcagct actaattttg tagttttcct
     1561 gtgtcgctcg gttcggcctc gctttggcgc agcagcatct ggctgatgag tgcgcgcagc
     1621 gccgatgggc gcacgggctt gagcagaaag ccccagccgc gctcggcggc ctggcggcgc
     1681 agggcggtat cgcgctcggc cgtgaccagc accaccaggg ggtgctgctg ccagcgcgcg
     1741 cacaggtcgc cgtacagatc cgggccgtgg aaggcgccca tgcgcacgtc cagcagcacc
     1801 aggcgcggcg cgttgccggg cgccgccaga tccagcgccg cctgcgggcc gccggccagg
     1861 ggcacgtcgc agccccagcg ctgcaaaagc gcgccggtgg cggcgcagct gggggcgtcg
     1921 tcgtccacat accaggccgt ggtgccttgc aggggttgat cctgtgcggg ctcgctctcg
     1981 gtgggcgcca gcgatggcgc ggcgcccacg gtggccacga tgggcacgct gacccagaac
     2041 atgctgccgc gcccgagctc cgagcgcagg ccgatctcat ggcccagcag ccggcccagg
     2101 cgctcgacga tggccaggcc caggccggcg ccgcggtcat cgtcatggcc ctcgcccagg
     2161 cggcggaact cctcgaagat ctcgcgctgc aggctctcgg gtatgcctgg gccctggtcg
     2221 tgcacctcga tgcgcagctg atcgccatgg cgccggcagc cgatcagcac cttgccgccg
     2281 ggcgtgtagc ggatggcatt ggagagcagg ttttgcaaga tgcggcgcaa gagcgcctcg
     2341 tcgctgcgca ccgtgtagcg cgagcccacg cagtccaggc gcaggccgcg gctcttggcg
     2401 atgatgccga agttgtgctg caccacctgc agcagcggcc cgagcgcgaa gtcgcgcagg
     2461 cgcacctcca gctggcccga ctccatgcgc gagatgtcca gcaggctggc caggatgccg
     2521 tcctgcgcgg ccagcgcgcc gtcaatgctg tcggccacct ggcgcccggc ctcgtccagc
     2581 gcatggccgc gcaacagcga gctgaacatg cgcgccgcgt tcagcggctg cagcaggtcg
     2641 tgcacggcgg cggcgacgaa gcgcgtcttg tagcgattgg cctccacggc ctcgcgccgg
     2701 gcggcgtcca ggtcgcgcgt gcgctcggcg atgcgcagct ccagcgcgtc ggccagcgag
     2761 cgcagctcgc gcgcggcgtt cttgtagctg gtgatgtcgg catagctggt gacaaagccg
     2821 ccctcgggca gcgggttgcc gcgaatctcc agcacctggc cgtcgctctt ggagctttcg
     2881 tgcaggtagg gcgtgccctc gcgcaggtgg cgcaggcgcc gctgcacggc gtcgtcggcc
     2941 ggcggcggcc caagcaggcc gcggcgcgcg ttgtagcgca gcagggcctc tatcggggcg
     3001 cccacgcgca ccagctccgc agggaagcga aacagctcga cgtagcgcga gttccaggcc
     3061 accaggttca gctccttgtc caccaccacc acgccctgcg gcaggtgctg caggctgcgt
     3121 gacaggccgg tgtcggcctg gcgtgcggcc tgcacgatgg cgccctgggc ggtgcgcagc
     3181 tcttgggcgt gttgttgcag gacggtctcc agctcctggc ggctgcgttg gcgcagcagg
     3241 gccaggcgcc ggcgctggcg caggatcagc accagcagcg ccagggccag ccacaggccg
     3301 ccagcggcca cggcggccca gcggccggcg ctggccacgg cgcgggtgtc gtgcagcagg
     3361 tgcagcctcc agtcgctgcc gggtagctcc agggtctgcc acagcagcgg ccggtgctcg
     3421 gcggggtctt gcaggcgcac caccacaccg ccgccggcta ccgggcgctc gctgcggtac
     3481 agcaagggcg ccagaggctg gtcggcatat tggcgcgtgg cggccagctc gccgcggtcc
     3541 tgcgccgtga gcgggttgag cagccggtag cgccaggcgt cgcggctggc caggaagacc
     3601 acgccatggg tgtccgaggc gagcacgatg tcgggcccat tgcgccactc acgctccagc
     3661 tcggtgaaga cgatcttcac ggccaccagg cccagggcgc gcccctgcgc gtcgcggatg
     3721 gcggtggaca ggaagtagcc cggctccccc gtggtcaggc ctatgccgta gaagcgcccc
     3781 tggccgtgct gcagcgcctg ctgcacatag gggcggaagt ggtagttctc gccgacgttg
     3841 ctgtgcgcgt cgcgccagtt gctggcggcc agcgccaggc cgtcgcgggc aatcagggtc
     3901 agcgtggagg actggctggc gccgttggcc tgttccagct tgtggttcag cgcctgcacc
     3961 gccgccggcg tgagcggttg cgccagcgcg gtgcgcagct cgctgtccag cgccagcacc
     4021 tcgggcaggg tgcgaaagcg gtctatgcgc tgcaccaggg cctgggcgta gagcgacagc
     4081 cggcgctgca catcctggct ttcctcattg agcgcgcgcc gctgcacgat ctggccgacc
     4141 agcagcatgc acagcgccat gccggcgagc acgacgagca cggtccacag cagttggcgg
     4201 cgttggttca tgggcctgga gtgtgcgcgc ttgggccggc gcgggatgcg tcgcctaata
     4261 ccaataggtt atcggcaaaa gaaagctggc gatgtccaat tcaccacaac gatgcgccat
     4321 gcgagcgcgt cattttctgg cccacgaaag acccgccatg cacgccattc ccctgcaaga
     4381 caccgccgcg cccgagcgcc tgccctttta ccgccagctg tatttccagg tggtgctggc
     4441 cattgtgctg ggcgtgctgc tggggcattt cgagccgcag tacggcgcgc aattcaagcc
     4501 gctgggcgac gccttcatca agctggtgaa gatggtcatc gccccggtga tcttcctgac
     4561 catagtcacc ggcatcgccg gcatgacgca ccttcgcacc gtgggccgcg tgttcggcaa
     4621 ggccatggcc tactttttgt gcttctccac cctggcgctg atcgtcggca tggtggtggc
     4681 gaacgtgctg cagcccggcg ccggcatgca catccgcgtg gcggatctgg acaccaaggc
     4741 ggtggacagc ttcgtcgcca agtcgcatga cctgagcctg gtcggctttg ccatggacat
     4801 catccccaag accctggtca gccccttcgt cggcgacaac atcctgcagg tgctgttcgt
     4861 ggccgtgctg ttcggcgtgg ccctggccat ggcgggcgac gccggaaagc cggccctgaa
     4921 cttcttcgag gcgctgacca agccggtgtt caagctggtg aacatcgtca tgaaggccgc
     4981 cccgatcggt gcgtttggcg ccatggcctt caccatcggc aagtttggcc tgggctcgct
     5041 ggtgaatctg gccgagctgg tggccacctt ctacctgacc tcgctggtct tcgtgctcat
     5101 catcctgggc gcggtggcgc ggctgtgcgg cttctccgtg gtcaagctga tgcgctacct
     5161 gaaggccgaa ctgctgctgg tgctgggcac gtcttcgtcg gaatccgccc tgccctcgct
     5221 gatggaaaag atggagaagg ccggttgtag caaatcggtg gttggtctgg tggtgcccac
     5281 gggctactcc ttcaacctgg acggcaccaa catctacatg acgctggccg cgctgttcat
     5341 cgcccaggcc accgacatcg acctgagcct gggccagcaa ctggccctgt tgctggtggc
     5401 catgctgtcg tccaagggtg cggccggcgt caccggcgcg ggcttcatca ccctggccgc
     5461 caccctggcc gtggtgcccg aggtgccggt ggccggcatg gcgctgatcc tcggcgtgga
     5521 ccgcttcatg agcgaatgcc gctctctcac caacttcgtt ggcaacgccg tggccacggt
     5581 ggtcgtctcg cgctgggaga acgggctgga ttaccagcgc ctgcatgcgg cgctggacgg
     5641 cgaagccgcg gccccgatga aggaaacgct ggcgggttaa gccttcgcgc ttgaagcaac
     5701 ggcccaggtt cgcctgggcc gttgtcattt gcggcctcag aacggcgcgt cgtcgtccgg
     5761 gccgctggtc ggggcgtgca gggcgccgtt tacgggcgtc gctgccccgc cgatctcgcc
     5821 gcgcccttcc ccgcccaggg ccaggatcag gtcggggatg agcttggaga gttcgcccgt
     5881 ggcaatcgcc acgtcggcgt caaagccggc atcgtcctgc ttctgaccct cgaaaacggt
     5941 gtcctggaag gcgattttct tcagctgcag gccctcggtg agcacaaagc tgacgcggtc
     6001 gtcccagctc atggccagtt tggtgggcag cttgccggcc tcgatatgcg cctgcacctc
     6061 ctcgatgtcc agcgggtggc gcgcgtagcg gaccacggcc ttgtcctcgc cggcgctctt
     6121 cagttcgcat tcgcggtcta tggaaaagcc cgccggcggc tcctggctct tgagccagtg
     6181 ggccatggcg gcctgggggc tggtctgggt gtccagcagc gccagcgaca ggccgggcag
     6241 ctgctccacc agcaggctgg tgatctcgtc agcgcggccc tgggcgccgg tgtccagcat
     6301 cagcaggccg gcctcgcggt ccagccacac ccaggtcgag ccctgcttgg tgaaggccat
     6361 gggcagcagg tccagcctgg cctcgtcctt gagctccttg gcctccttct tgccgggctt
     6421 gcggccggtc tgctgctcga tgagcgcggc cttctccttg accttgcgtg ccagcaccgt
     6481 ggccggcagc atcttggatt cggccatgaa gcgcagcatc cattgcccgc ccacgctctc
     6541 ggccagcggg ccgtgggcat cgccgcgcgg cggcacccag ccaacggcct tttcctgcgt
     6601 ggcgccacat tcctggaacg gggtcttggc cagggcctcc tccagctggg tcagatccgc
     6661 ctgccagccc ggggcaatgc ggtacacgat gaggttcttg aacacttggg ttattcctga
     6721 tttgatagct gtcaacgctt gatggtaaag cgcaaaagcc ctattttctt cataaaaatg
     6781 ctgcagatgc gcggcacggc ctggcagaac tttacacagg cgcagtgttc ccacatggtg
     6841 gcgttacacg ggcgccggac actggcgtcc atggttttgg tcacgcaaga aaggaattca
     6901 ccatgacccg tttccgtatc acccaacgcc tggccgccac cgccctggtg gccgccctgg
     6961 ccggcattgc cgctccctcc ttcgcccagc ccgcacccgg tgccggcccg gtggccgagc
     7021 aggcccagcg cccgcgcgcc atgacacccg agcagcgcca gcagttcatg aaggaacgca
     7081 tgggccagcg cctggaggcc ttcaagcaga agctgcagct cacgcccgcg caggagccgg
     7141 cctggaacgg ctttgtgcag tccatgcagc ccaatgccga gccgcgccat gcccgccttg
     7201 actggcaggg catggacaag ctgaccacgc ccgagcgcat agaccgcatg cgcgccatgc
     7261 acgcccagcg tggcgccgag atggaacgcc gcggcgaggc cgtgaaggcc ttctacgccc
     7321 agctgacgcc ggcgcagcaa aagaccttcg acgaccaggg cgcgcgcatg gttggccagc
     7381 gcggtgccaa gcaggggtgg ggcatgcatc gccacggcca ccaccatggc tttggccctg
     7441 gcgcatccat gggccagtaa tagacggcga cagccagggc tgcatgctgc ggccctggcc
     7501 tacaaggccc agggttggag ggggtctaca ttcgttacat ttgccctgca caaactaacg
     7561 aataaacgtc atgttccatc cttgtctgcc gcacccctcc ccttttcgtg gcgcccgcgg
     7621 cgccagccag cgccattggc atgccctggt tgcagcgggc tggcttgccg ccctgcccgc
     7681 ctttgcacag caggccatga ccgatctgtc gcacgcgccc agcatctacc tgcagggggc
     7741 catagcggag cacgacaccg aggccctgac cctgggtctg accctgccct ggcgcgaatg
     7801 gcgcatgccg ctgtggggtg gcgagctgcg cggccactgg gatctgtatc tcagccgctg
     7861 gttcttcaag ggtgccgcaa ggcatggcgg caccctcgtg ctgggcgtca cgcccaccct
     7921 gcgcctgcgg cccgatggcg gcagctctgc ctggttctgg gaggccggca taggtgccac
     7981 gctggccgat caacgctacc acccggcggg acaggaagag ttcagcacgc gctttaactt
     8041 tgcctcgcac ctgggcatcg gcatcaactt tggggcacga cgccagcatg agctgctgct
     8101 gcggctgcag catgtctcca acgccggcat caagaaaccc aacccggggg tggaattcct
     8161 gcagctgcgc tacgcctggc atttttgact tgcctcctgt gggcaaggct gtgcatgaaa
     8221 ggctggcaaa tggtggctta tccacagcca gcggcgcttg gcgcaagttg ttccagattc
     8281 gcggcgcgct tgcaccggcc tgaagccaca cccttggcag catcctaact atctgtgggc
     8341 attacagaaa acaggcttat ccacagcgca gggcgggctc tactactacc actatgtatt
     8401 gatgaatacc tatatggaat aagcgaggag gccatcggcg ccggtgccag cggcgttttt
     8461 tgcgctgata tgggactgca tcagctgctc gacctcctgc gcgggcaagg gcttgctgaa
     8521 tagatagccc tggtaggcct ggcagccggc ctgggccagc cagtggcgct gttcctcggt
     8581 ttccacgccc tcggcaatca cctccagccc caggctgtcg gccagcgcga cgatggtgcg
     8641 cacgatggcg gcgtcgttcg ggtcggacaa caggtcgtgc acaaagccct ggtcgatctt
     8701 gagctggccc agcggcatgc gtttgaggta ggtgagcgag gaatagccgg tgccaaagtc
     8761 gtccagcgaa aaactcacgc catgcgcgcg cagcgcctcc atggtggcga tggtcttggc
     8821 catgtcttcg accagcaggc tttccgtcag ctccagcttg agctgcgccg ccggggcgcc
     8881 ggtgacggcc agcacgcgca gcacgtcggc cacgaagccg gcatcgcgaa actggcgcgg
     8941 gctgacgttc acggccatgg tcaaaggcgc cagatcgggc tgtttttgcc acgccgccag
     9001 acgcgtgcag gcggtgtgca gcacccagcg ccccagtggc aggatgagac cggtctcttc
     9061 tgccgcgggt atgaactcgg cgggtgacac catgccgcgc cgtggctgcg tccagcgtag
     9121 caatgcctcc acgcccagga tctggccgcg cgcgttgagt tgcggctggt agtgcagcga
     9181 gaactcctgc tgcgccaggg cggagcgcag gtcggcctcg aacgccacat gctcgtccac
     9241 ggccttttgc atgcccgggt tgaaaaagcg cagcgtgctg cgcccggcca tcttggcctg
     9301 gtacatggcc aggtcggcct gcttgagcag ctctcccacg gtggtggact gatcatcgaa
     9361 caaggccacg cctatgctgg gcgtgctgcg gtactggtgg cccgccaggg catagggaag
     9421 ggagagcgcg ttcagtatct tctcgcccac cttgttcgca tgttcggcta gggttgcggg
     9481 ctgggcacct atctgttcga gcaacaccac gaactcgtct ccgcccagcc gcgccaccgt
     9541 gtccatggca cgcacgcagg tgttgaggcg ctgggccacg ctcttgagca gcaggtcgcc
     9601 ctggtcgtgg cccagggtgt cgttcagctg cttgaagttg tccagatcga tgaacagcag
     9661 cgccccaccc tggcgctggc gctgggccgc ggccagggcc tggcccatgc gttgcatgaa
     9721 atgcgcgcgg ttgggcaggc cggtaagggc atcgaacagc gccaggcgct ggatctcccg
     9781 ttcgctggcc ttgcgctggc cgatgttgga gttgatggcc agaatggcct cggcctcgcc
     9841 ctgttcgctg cgcaccaggg tccagcgccc ttcgacttcg atggcgcggc catcgcgcgc
     9901 atattgctgc agctcacccg tccattcgcc gttgcgcagc acctgggcgc tggcttggtg
     9961 gaagcactgt gcatcctggt agagcaggct ggcggcgttg tgccccaatg cctctgcgcg
    10021 gctccagccg tacagccgtt ctgcgccctc gttccagaag gtaatgcgat gctccaggtc
    10081 gcgcacgatg atggcgtcgc tggccttgtt caagagcgat gcctgctggc gtatgcgcat
    10141 gtcgtccgcc tggcgctgca gttcggcagc ggcgcgggcc gcgaagatct gcagtgcggt
    10201 gtcaacgaga tgcgctcgcc gcaacggctt gcggtacatc accaggatca tgcccagggg
    10261 cgtgccgtcg ctgcccgtga gttgccgccc gacatagctg cgcaccctgc accgtgccag
    10321 cacggggtca tgcggaaaca gctgggccac gcggtcttcg atcacgacca ggggctgggt
    10381 gagcaattgc gcgctgggtg tgcccgacag cgcgtattcg agcgtgggct gcaccacgcc
    10441 atcgctgata tgcgacagcg tcacggcctg caggctctgg ccatccacgg gcggctgcag
    10501 gcgcaccacg caggcaacct gggccgacag cgcgtctgcc atatgctgcg ccaggtggtc
    10561 gaagaaggcc acgcccgttc ctgccgatac cgccttggcc accttcacga tggcggcctg
    10621 caggcgttgc tgctcggcgc gggtgcgcag gttttccagg ccaaaggcca ggtcggcggc
    10681 catggcttcg agcagctgcg tctcctgcgc acccggctcc agcacctcgg gggcatacag
    10741 gcacagcagg cccaggttgc gctctgcgcc gcgcagcggc aggtagatgg cggaatgaaa
    10801 ctccagcgcg tccagcagcc cggccacctt gtcgaaggtg gggttggcgc gcatgtcgcg
    10861 caggacgacg gtgcggccgc tgcgcacgca gatgctggcc ggcccctggc caccgggctc
    10921 gtcgggcgac cagctcaggc gcagctgcgc caggtattcg ggcgagcctc cggcatgggc
    10981 cacgggttct atgcgcttgc gctcgtcatc acgcgccagg cccacccagc ccatgcggta
    11041 accgcctacc tgcaccgcca ccgcgcacac ggcgtgcagc agctcggcct cggtgttggc
    11101 gcgcagcagg gtctcgctgc aggcactgcg catgcgctgg gcccggctca ggcgcgccag
    11161 ttcggcgtct atgcggctgc gctcggtgat gtcatgggcc agtatctgcc aggccgggtg
    11221 accgtcgaaa tcggtgacgc cgacgaatac ctccatgtcc atcagcgtgc cgtccttgcg
    11281 tgcgtggcgc caggtggtag ctgccgtggc cagcgtgtgc gcggcgcgcg cgtcgctgca
    11341 catggccagg gcgctgtcgg cctgggcgcg ctgtgcctgg ggccagagca ggcgcatgtc
    11401 catgcccagc agctcctgct cgtcatagcc atagtggcgc gccatggccg ggttcacggc
    11461 cagcaggcgc aggctgccct ggtcacagac ccacatcggc tgcgggtgct cggtgaacag
    11521 gctgcggtac tgctgcccgg cctgccagtg caggctgatg tcgatcatgc cgccgaccat
    11581 gcgcaggcca cggccgctgg cgtcgcgcag cacgaagccg cggtcgtcca cccaggcgta
    11641 gctgccgtcc tggcggcgaa agcggtactg ggcgcaccag aactcagcag tgccggctat
    11701 gacttgccgc agactttgct gcacctgtgg cgcgtcgtcg gggtgcaggc gcagcgtcca
    11761 cgagctgccg tccggcggca ggctggccag gggcacgcca aagagccttt ccatgccgtc
    11821 gttccagcgc atggtgtcgg actgcagatc ccgatcccag aaggcggagg tcatggcgcg
    11881 aaatcccagc gcgcccgccg cagccatgtc gggcctgtgc gtcatgcccg cacccgcaaa
    11941 accccgcccc tggccgcgct gcatgggcgg caggcgggtg gcagtgggca ggggtttgcg
    12001 ctcatggcct aggtcaaggc aatgcggcgc aagcggccga cggtggcagg acaggtgggc
    12061 tcaacggcat ggtgtggcag ggcatggtga ggggacggcg gaaaccatgg tttacctgag
    12121 gttacagcca atccaccatg gggctgaacc ctcatttgca ggatggggtg cccgcggttt
    12181 cgccgagacg ggcacctcgt ggttgtaaca agcaggcatg ccaactcctt gctgcagcaa
    12241 agtatccatc gggtcaggct tgccacggcc cccggatttc cacgcccccg cccgctccgc
    12301 tatggggtgt tgcgctcgcg ccgcgcgcgc acggcagcgg ccagctgccg cagcaccggc
    12361 accgtctgcg caaagctgat gcaggcatcg gtcaccgaga ccccatgcct gagcgcctgg
    12421 ccgggcacga tgtcctggcg gccttcctcg aggtggcttt cgatcatcac gccggtgatg
    12481 cgcgcatcgc cgcccgcgat ctgtgcagcc acatcctcgg ccacggcgat ctggcgtgca
    12541 tgctgcttgc tgctgttggc gtgcgacagg tcgatcatca cctgcgcttg ctggccggac
    12601 ttttgcagca gctcgcaggt cgattgcacg tcggcggcgc tgtagttggg tgccttgccg
    12661 ccgcgcagga tcacatggca atcgtggttg ccgcgcgtct cgaagatggc ggcctggccc
    12721 atcttggtca tgcccatgaa ggcatgctcg gcctgcgcgg cctgaatggc atccacggcc
    12781 accttcacgc cgccgtccgt gccgttcttg aagcccaccg ggcaggacag gccgctggcc
    12841 agctggcggt ggctctggct ctcggtggtg cgcgcgccga tggcggccca gctcaccagg
    12901 tcgctgatgt actggggact gagcagatcc aggaactcgg tgcccacggg caggcccagc
    12961 gccagcacgt ccagcagcag caggcgcgcg cgctccagcc cctcgttgat ggcaaagctg
    13021 ccgtccaggt gcgggtcgtt gatatagccc ttccagccca cggtggtgcg cggcttctcg
    13081 aagtacacgc gcatcacgac aagcagatcc tgcgccagcg cgtctgcctc ttgcttcagc
    13141 agccgcgcgt actccatggc ctggtcatgg tcgtggatgg aacagggccc gacgacgacg
    13201 atcagccggt cgtcctggcc gtgcagcacg cgcgacagct gcaggcggct ggcctccacc
    13261 agctcctgcg cggcgtcggg cgcgggcagc cattcctcca ggatggcggg tgtgatcagg
    13321 gggcgcacgg ccttgatgcg caggtcgtcg atgcgcgtgg tgtcgtgcgt gctgggcgtg
    13381 gggatggggc ggtcgatgag tgtcatggtg gcccgattgt cgcccctgcg cggcgcgccc
    13441 gttcaggggc gccggtagct ggcggactgc tgcgcccagt agcgcgagcg caaatgatcc
    13501 agccgcaccg tgccaccgct cgatggtgcg tgcacgaagc gcccttcgcc cacatagatg
    13561 ccggcatgcg tgggccggcc gccgccgaac aacaccaggt cgccggtgcg cagctcgtcc
    13621 tccggcaccg acgcgccaaa accgctcagc agcgccaccg tgcgcggcgg ggccacaccg
    13681 gcgcggctgt tgtagacata gccgatcagg ccgctgcagt caaagccgcc ctcgggtgtg
    13741 ttgccgccaa agcggtaggg cgtacccacc aggcccaggg cgtggatggc gatgtcgctc
    13801 gattgctcgg cagacagccg ggcataggcg ctggagcgcg cggcgggcgc cgtgccgcag
    13861 cccgccagca acaggccggc cgcgagcagc aaaacagggg gaacgagctt gcgcatggct
    13921 gcaagcctag cagcctgcca ccgccgttgg tgcagccgcc gccaaggccc cggctttcag
    13981 ctactttttc agcggcattt cgggttgtat ggccctattt ctgtttccgg ctgtgacggg
    14041 cgccagtttt cttgaccgat tcggcggcat atcgcgcgca tggcagtaca aaaaatcgta
    14101 ctagtacaaa caatacaatg tacctatgac atctttgttc gtcgaaggaa gcaatgccag
    14161 agacattgcc acaagccttg aacatgccat gcgcagcggc gccctgccac ccggctctgc
    14221 cctgcccccg gtacgccagc tggccgttga gctgggcgtg aatcccaaca ccgtcgcggc
    14281 cgcctatgcg cggctgcgcg ccgccggccg cgtgctcacg gcggggcgca agggcacgcg
    14341 cgtggccggc gcgccgccct cccccactcc ggtctatgcc gcgcccgagg ggctgcgcga
    14401 cctggccggc ggccaggtcg acgccgccct gctgccgcgc ctacgtggcc aggcctggac
    14461 gcaggcgctg gcccaacccc tggatggcgt ggctggcgat gcaccgctgc tggctcaggc
    14521 cgcgcactgg ctgcagggcc agggtctgcc gggcgaagac ctgggttgtt tctcgggcgc
    14581 gctcgacgcc atcgagcgcg ccctgcgcca gcatgcgcgt cccggtgacc gcgtgggtgt
    14641 ggaagacccg ctgtggccgc cgctgctggc gctcttgcac agcctgcgcc tggtgccggt
    14701 ggcgctggcc gtcgatgcgc agggcatgtg cgtgccggcc gatggcgtgc tgagggactg
    14761 cgccgccgtg gtgctcacgc cgcgcgcgca gcaccccacg ggcgtggcca tgacggcggc
    14821 gcgctggagg gcgctgcgcc gcgccttgcg tgcctgcccg cacacactgc tgatcctgga
    14881 cgaccactgg ggccccttga gcgccgcgcc gctggccatg gccggcaggc tgccgccgct
    14941 gtggctgcat gtgctgtccg tcagcagcag cctggggccg gactgccgcg tggccgtggt
    15001 cagcggcacg ccagggctgg tgcagggcat gtgcgcgcac caggcgctgg gcccgcgtgc
    15061 cgtcagccgc tggctgcagg gcctggtggc gcagctgtgg cgccaggcca gccggggcag
    15121 ggggcgtggc tcctggcagc acatggccgg cagctacgcc gcgcgccgtg tg
//
LOCUS       DAOMTQ010000065        15102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_622_length_15102_cov_27.078089, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000065 DAOMTQ010000000
VERSION     DAOMTQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_15102_cov_27.078089"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..312
                     /locus_tag="PK518_08095"
     CDS             43..312
                     /locus_tag="PK518_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48914.1"
                     /translation="MKNLTITVEDNVLEWARIEAARRGTSVSRMVGDFMAEMMQREDA
                     YERAYLAWRTDERSWQAQVAAKSIARRASAARTSGQNNQKTAEGA"
     gene            309..755
                     /locus_tag="PK518_08100"
     CDS             309..755
                     /locus_tag="PK518_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRM48915.1"
                     /translation="MNPVFVDTSVLIAAEDTAGGALYQACLDWLDLLWRTRSGRTGCQ
                     ALVEFYDQVTAAPQPLPRGDARAAMRRYQTWTPWKTDAATLETAWAIEARHALAFGDC
                     LQLACAQHSGCAAMLTLALPHGAVFAGVAVWHPLQQALPALEKDPQ"
     gene            761..1864
                     /gene="hisC"
                     /locus_tag="PK518_08105"
     CDS             761..1864
                     /gene="hisC"
                     /locus_tag="PK518_08105"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRM48916.1"
                     /translation="MSTPLQQAALARIRPDVRAMHAYVVQGADGMLKMDAMENPFALP
                     PALQAQLGQRLGALALNRYPGPRGDELRAALARHAGMPEGCALMLGNGSDELISLLAL
                     ACAQSGATVLAPVPGFVMYAMSAQLQGLSFVGVPLTADFALDESAMLAAIAQHRPAIT
                     FIAYPNNPTATLWDEGAVQRIVDAVGAQGGIVVMDEAYQPFASRSWIERMRAEPARNG
                     HVLLMRTLSKFGLAGVRLGYLIGPRQLVAEVDKVRPPYNISVLNCEAALFALEHAEVF
                     AAQAAEIRAERERIVPLLRAMPGVQKVWDSQANMVLLRVRDAASAFEGMKARKVLVKN
                     VSTMHFLLANCLRLTVGSPADNAQMLAALEASL"
     gene            1861..2493
                     /gene="hisB"
                     /locus_tag="PK518_08110"
     CDS             1861..2493
                     /gene="hisB"
                     /locus_tag="PK518_08110"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRM48917.1"
                     /translation="MTSSALVPSNPEPGAERIAEVSRNTAETRISVRVNLDGTGAAHL
                     HTGIGFFDHMLDQIARHGLIDIDVDCDGDLHIDGHHTVEDVGITLGQAFARAVGDKKG
                     IRRYGHAYVPLDEALSRVVVDFSGRPGLHLHIPFTAASIGGFDTQLTYEFFQGFVNHA
                     GVTLHIDNLKGINAHHQCETVFKAFARALRDALARDPRAGGVIPSTKGSL"
     gene            2545..3213
                     /gene="hisH"
                     /locus_tag="PK518_08115"
     CDS             2545..3213
                     /gene="hisH"
                     /locus_tag="PK518_08115"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRM48918.1"
                     /translation="MNNIVKTVAIVDYGMGNLRSVSQAVQAAAQGSGWHVAVTADPDV
                     VHAAARVVLPGQGAMPDCMRELRESGLLESVLEAAATKPLFGVCVGMQMLLDHSAEGD
                     VAGLGLIPGEVLKFDLAGRTQADGSRFKVPQMGWNRVRQMRHGGQLHAMWAGVPDDSY
                     FYFVHSFYARPANPAHCAGQADYGAPFAAAIARDNIFATQFHPEKSAEHGLALYRNFL
                     HWKP"
     gene            3301..4044
                     /gene="hisA"
                     /locus_tag="PK518_08120"
     CDS             3301..4044
                     /gene="hisA"
                     /locus_tag="PK518_08120"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRM48919.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQSTTFGEDPAAMARKWLDAGA
                     RRLHLVDLNGAFAGQPKNKAAIKSILAEVGADIPVQLGGGIRDLDTIERYIDAGLEYV
                     IIGTAAVKNPGFLKDACSAFAGHIIVGLDAKDGKVATDGWSKLTGHDVVDMAKKFEDW
                     GVESIIYTDIGRDGMLSGINIEATVRLAQALSIPVIASGGLAGIADIEKLCEVEIEGV
                     EGVICGRAIYSGDLDFASAQARADELAGA"
     gene            4326..5342
                     /gene="galE"
                     /locus_tag="PK518_08125"
     CDS             4326..5342
                     /gene="galE"
                     /locus_tag="PK518_08125"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRM48920.1"
                     /translation="MILVTGGAGYIGSHMCVALAQANESCLVLDNFSNTRPSVLERIG
                     RITGQAPECVEGDIRDAALLKQLFANNKISAVIHFAALKAVGESVREPLRYYENNVAG
                     TVTLLQAMQRAGVRQLVFSSSATVYGDPASLPIREDFPLSATNPYGWSKLMMEQMLAD
                     LDASEPGQWRIARLRYFNPVGAHESGLIGEDPQGVPNNLLPYVAQVASGQREFLNVWG
                     NDYSTPDGTGQRDYIHVCDLAEGHVAALRYLRQHPGLLTVNLGTGRPVSVLEMVRGFE
                     AASGRPVRYQIGPRRAGDVAACWADPGLAERLLGWRARRDVQAMCQDAWRWQDGVARQ
                     LQGA"
     gene            5383..6162
                     /gene="hisF"
                     /locus_tag="PK518_08130"
     CDS             5383..6162
                     /gene="hisF"
                     /locus_tag="PK518_08130"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRM48921.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDGRDLILPIIEAVASQVFIPLTVGGGVRTVQDVRRLLNAGADKTSF
                     NSAAIANPEVINQASDKYGAQCIVVAIDAKRRQGQDLAERGAGWDVYSHGGRQNTGLD
                     AVRWAVDMARRGAGEILLTSMDRDGTKSGFDLQLTRAVSDAVDVSVIASGGVGNLDHL
                     ADGVSIGGADAVLAASIFHYGEFTVRQAKEHMHARGIPVRL"
     gene            6265..6654
                     /gene="hisI"
                     /locus_tag="PK518_08135"
     CDS             6265..6654
                     /gene="hisI"
                     /locus_tag="PK518_08135"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRM48922.1"
                     /translation="MNWLDQVKWDAQGLVPVIAQEAATGDVLMFAWMNREALARTAEL
                     GRAVYYSRSRGRLWFKGEESGHVQQVHEMRLDCDNDVVLLKVTQLGHEPGIACHTGRH
                     SCFFSVLKDGAWQAVDPVLKDPESIYK"
     gene            6660..7037
                     /locus_tag="PK518_08140"
     CDS             6660..7037
                     /locus_tag="PK518_08140"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRM48923.1"
                     /translation="MSSTDSLARLAAVIESRKSANGGDPQASYVARLLHKGPDAFLKK
                     IGEEATEVVMAAKDVDHGAAPAKLVYEVADLWFHSMIALAHYGLAPADVIAELERREG
                     TSGIEEKALRKVAGRALEEGEAP"
     gene            7034..7420
                     /locus_tag="PK518_08145"
     CDS             7034..7420
                     /locus_tag="PK518_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48924.1"
                     /translation="MSNDIIDMEPDERAESLKTIGWVSYILHLIVAVGAVMPGAQPGV
                     ALLIIALVIDLVKKGDAEGTWQASHFSWRISTVLWAGVLYVVTAPLWLFFVFPGWVAW
                     GLISIWFLYRIVRGMVAMNKGQAIDV"
     gene            7413..8441
                     /locus_tag="PK518_08150"
     CDS             7413..8441
                     /locus_tag="PK518_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRM48925.1"
                     /translation="MSEVAATPPLNLALQGGGSHGALTWGVLDALLEDGGFTFEGISG
                     TSAGAMNAVALAHGFAQAALQHKDAHEAHQAGCALARQTLAQLWEGVGTMGSLLWGVP
                     LQGNPFLGMLSQWLSPYQTNPLGINPLRGLLERVVDFDALCHARHALVPKVFVCATNV
                     RTGRGEIFSGARLSADAVMASACLPLLFKAVQIDGEHYWDGGFSGNPALYPLIYQTQC
                     ADVLLVQINPIEHPDLPDTAPEIMERMNEVTFNASLLGELRAIDFVRRLLAEGRLDPQ
                     HYKSMRMHRIDGGRVLAPFGDASKSRADMAFVRQLFELGRTQGKQWLRRHRRDVGVQH
                     TLHLTDNH"
     gene            8474..8830
                     /locus_tag="PK518_08155"
     CDS             8474..8830
                     /locus_tag="PK518_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRM48926.1"
                     /translation="MHDPNCLFCKIIAGQIPSKKVYEDEQVFAFHDIHPWAPVHFLIV
                     PKLHLPSMASATPEHAGLLGHMMLLAPRLAIEQGCNPYPDGGYRIVVNTGTEGGQEVH
                     HLHMHVIGGPRPWLRG"
     gene            8915..9160
                     /gene="tatA"
                     /locus_tag="PK518_08160"
     CDS             8915..9160
                     /gene="tatA"
                     /locus_tag="PK518_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HRM48927.1"
                     /translation="MGSFSIWHWLIVLLIVVMVFGTKKLKNIGSDLGGAVKGFKDGMK
                     EGASSDEQAGANAPAGQVTNTTAADKTTIDVQAKQKS"
     gene            9183..9662
                     /gene="tatB"
                     /locus_tag="PK518_08165"
     CDS             9183..9662
                     /gene="tatB"
                     /locus_tag="PK518_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HRM48928.1"
                     /translation="MIDIGLSKMALIGAVALIVIGPEKLPRVARTVGTLLGKAQRYVS
                     DVKAEVNRSMELDELRKMKDTVEGAARDVEQSIQTSASDFQKDMESSLGEDFAPLGEP
                     SGAQELSSVVPAYKHPGKNWRLKRGAMPQWYKARAGVRTKAQSGAARVARFRPQKFH"
     gene            9732..10520
                     /gene="tatC"
                     /locus_tag="PK518_08170"
     CDS             9732..10520
                     /gene="tatC"
                     /locus_tag="PK518_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRM48929.1"
                     /translation="MPETPNPEDELAGTEQPFVQHLMELRDRLVKAMIAIGVVAAALF
                     FYPGPGQLYDLLAAPLVAHLPQGATMIATSVISPFMVPLKILLMSAFLIALPFVLWQV
                     WAFVAPGLYAHEKRLVLPLVVSSTVLFFIGVAFCYFFVFGQVFSFIQGFAPKSITAAP
                     DIEAYLGFVMTMFLAFGLAFEVPIAVVVLARMGVVSVEKLKSFRGYFIVVAFVIAAIV
                     TPPDVVSQLALAIPMCLLYEVGIWAAQVFIRHTQAPEESQESTS"
     gene            complement(10588..11727)
                     /locus_tag="PK518_08175"
     CDS             complement(10588..11727)
                     /locus_tag="PK518_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM48930.1"
                     /translation="MKRYWLLFSQAVTVLAAAYFVVATLQPGWLQRGATTSGAGISLI
                     EAPPSTGAQPAAGSFSAAARKAAPAVVSINTSKEVRHPRSNDPWFQFFFGDQGAQAQT
                     GLGSGVIISPEGYILTNNHVVEGADEIEVTLTDSRRASATVIGTDPDTDLAVLKVQLD
                     KLPVIVLGDSDKLDVGDQVLAIGNPFGVGQTVTSGIVSALGRNQLGINTFENFIQTDA
                     AINPGNSGGALVDVNGNLMGINTAIYSRSGGSMGIGFAIPVSTAKLVLDGIVRDGQVT
                     RGWIGVEPGELSPELAQTFGVQATRGVIITGVLQGAPAAQGGIRPGDVILSVAGKPTD
                     NVSQLLTAVAGLQPGKAASFELQRGDRQLELSVVPGTRPRMQQRR"
     gene            11816..12568
                     /locus_tag="PK518_08180"
     CDS             11816..12568
                     /locus_tag="PK518_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRM48931.1"
                     /translation="MSIQRSELLAHFDALLQPERFKDYGPNGLQVEGAQTIRRIISGV
                     TASRALIDAAIAARADAIFVHHGLFWRGMDGRVTGWMKQRLQRLLAHDINLFAYHLPL
                     DAHPELGNNAQLGRVLGWQADQSFGEQDLGFAAPAAFASAAELAAHVQATLARGVTLV
                     APEGAHPMRRVAWCTGGAQGFFESAIAAGADAFITGEISEPQTHLARETGVAFIAAGH
                     HATERYGAPAVAAHVAQQLGLEHQFIDIDNPA"
     gene            12632..13675
                     /gene="pdxA"
                     /locus_tag="PK518_08185"
     CDS             12632..13675
                     /gene="pdxA"
                     /locus_tag="PK518_08185"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRM48932.1"
                     /translation="MSTSLPLAITQGDPAGIGPEIVAKAFRDAPRDMRGCFVVGDLAT
                     LRRAAACIERPGDTSLPLARIDALQDVALVPPRCLPVWQLPELAGGEREPWGRVSAGA
                     GRAAAACVLWAARAALAGQVAALVTAPLHKESLAAAGVPFPGHTELLQAEAARHAGVP
                     IAQMPVRMMLASDELRTVLVSIHMSLREAIAAVTPDNLLQTLRITHAALSRSLGRAPR
                     IGVAGLNPHAGEGGLFGREELDIIAPAIAQARAEGMDAHGPYAPDTIFMRARCTAGRP
                     GEFDVVLAMYHDQGLIPVKYLGVDKGVNVTLGLPLVRTSPDHGTAFDIAGRGVADAAS
                     LIEAVRMARALAG"
     gene            complement(13743..14171)
                     /gene="mscL"
                     /locus_tag="PK518_08190"
     CDS             complement(13743..14171)
                     /gene="mscL"
                     /locus_tag="PK518_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRM48933.1"
                     /translation="MSITKEFKEFAVKGNVIDLAVGVIIGAAFGKIVDSVVADLIMPI
                     VGLVFGKLDFSNLFIVLGSIPEGTARTLDALKKAGVPVFAYGSFITVAVNFIILAFII
                     FMMVKQFNRLKREAPAEPVPEAVTPEDIVLLREIRDSLKR"
     gene            14315..14914
                     /gene="petA"
                     /locus_tag="PK518_08195"
     CDS             14315..14914
                     /gene="petA"
                     /locus_tag="PK518_08195"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRM48934.1"
                     /translation="MSETPIDSSKRTWLIASGCAGAVGGVATAVPFVSSFQPSEKAKA
                     AGAAVEVDISALKPGEKVTVEWRGKPVWIIRRTPEQVAELPKLDSQLADPQSKRNPAE
                     FTPPYAQNEHRSIKPEILVAVGICPHLGCSPSDRFTPGPQPSLPNDWEGGFLCPCHGS
                     TFDMAGRVFKNKPSPDNLPVPPHMYLSDTKLLIGEDKKA"
     gene            14930..>15102
                     /locus_tag="PK518_08200"
     CDS             14930..>15102
                     /locus_tag="PK518_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HRM48935.1"
                     /translation="MAAYREFKEISPNASATAKTMNWLENRFPTAFDAYKVHMSEYYA
                     PKNFNFWYIFGSLA"
BASE COUNT         2555 a         4972 c         4997 g         2578 t
ORIGIN      
        1 acacgaaatt cgtgcaacac gaatttcgtg tacgatggag ccatgaaaaa cctcaccatc
       61 accgtcgaag acaacgtgct cgaatgggcg cgcattgagg cggcgcgccg gggcaccagc
      121 gtctcgcgca tggtgggcga cttcatggcc gagatgatgc agcgtgagga cgcctacgag
      181 cgtgcctacc tggcctggcg cacggatgag cgcagctggc aagcccaggt tgcagcaaag
      241 tcaatagcgc gtcgcgcaag cgcagcaagg actagcggcc aaaataacca aaaaaccgcg
      301 gagggcgcat gaacccggtg ttcgtcgata cctcggtgct catcgcggcc gaggacacgg
      361 ccggcggcgc gctctaccag gcctgcctgg actggctgga tctgctctgg cgcacgcgca
      421 gcggccgcac cggatgccag gcgctggtgg agttctacga ccaggtgacg gccgcgcccc
      481 aaccgctgcc gcgcggcgac gcccgcgccg ccatgcgccg ctaccagacc tggacgccct
      541 ggaagaccga cgccgcgacg ctggagaccg cctgggccat cgaagcgcgc catgcgctcg
      601 cctttggcga ctgcttgcag ctcgcctgcg cccagcacag cggctgcgcg gccatgctca
      661 ccctggccct gccgcatggc gcggtgtttg ccggcgtggc cgtgtggcac ccgctgcaac
      721 aggccttgcc cgcattggag aaagacccgc aatgaacgaa atttccaccc ccttgcagca
      781 ggcggcgctg gcgcgcatac gcccggatgt gcgcgccatg cacgcctatg tggtgcaggg
      841 cgccgacggc atgctgaaga tggacgccat ggagaacccc tttgccctgc cgcccgcgct
      901 gcaggcgcag ctgggccagc gcctgggcgc cctggcgctg aaccgctatc ccggcccgcg
      961 cggcgacgag ctgcgcgccg cgctggcgcg ccacgccggc atgcccgagg gctgcgcgct
     1021 gatgctgggc aatggctcgg acgagctcat cagcctgctg gccctggcct gcgcccagag
     1081 cggcgccacg gtgctggcgc cggtgccggg ctttgtcatg tatgccatga gcgcccagtt
     1141 gcagggcctg tccttcgtcg gcgtgccgct cacggcggac tttgcgctgg atgaatccgc
     1201 catgctggcg gcgattgccc agcaccgccc ggccatcacc ttcatcgcct accccaacaa
     1261 ccccacggcc acgctgtggg acgagggcgc ggtgcagcgc atcgtggacg ccgtgggcgc
     1321 ccagggtggc atcgtggtca tggacgaggc ctaccagccc tttgccagcc gcagctggat
     1381 agagcgcatg cgggccgagc cggcgcgcaa cggccatgtg ctgttgatgc gcaccttgag
     1441 caaatttggc ctggcgggtg tgcgtctggg ttacctgata ggcccgaggc agctcgtggc
     1501 cgaggtggac aaggtgcgcc cgccctacaa catcagcgtc ttgaactgcg aggcggcgtt
     1561 gttcgccctg gagcatgccg aggtcttcgc cgcccaggcc gccgagatcc gcgccgagcg
     1621 cgagcgcatc gtgccgctcc tgcgcgccat gcccggcgtg caaaaagtgt gggacagcca
     1681 ggccaacatg gtgctgctgc gcgtgcgcga tgcggccagc gccttcgagg gcatgaaggc
     1741 gcgcaaggtt ctcgtcaaga acgtttctac aatgcatttc ctgctggcga actgcctgcg
     1801 tctgacggtg ggcagccctg ccgacaatgc ccagatgctg gcggcgcttg aggcctccct
     1861 atgacttctt ccgcacttgt accctccaac cccgaacctg gcgccgagcg catcgccgag
     1921 gtcagccgca acacggccga gacgcgcatc agcgtgcgcg tcaaccttga cggcacgggc
     1981 gccgcccact tgcacacggg cattggcttc ttcgaccaca tgctggacca gatcgcgcgc
     2041 cacggcctga ttgacatcga cgtggactgc gatggcgacc tgcacatcga cggccaccac
     2101 acggtcgaag acgtgggcat caccctgggc caggcctttg cccgcgccgt gggcgacaag
     2161 aagggcatac gccgctacgg ccatgcctat gtgccgctgg acgaggcgct gtcgcgcgtg
     2221 gtggtcgatt tctccggccg cccgggcctg cacctgcaca ttccgttcac ggcggccagc
     2281 atcggcggct tcgacaccca gctcacctac gagttcttcc agggttttgt gaaccacgcg
     2341 ggcgtgacgc tgcacatcga caacctgaag ggcatcaacg cccaccacca gtgcgagacg
     2401 gtgttcaagg ccttcgcacg cgcgttgcgc gatgccttgg cgcgcgatcc gcgcgcgggg
     2461 ggcgtcatac cgtccaccaa gggttccttg taaacattca agtcaaaatg gcctgtaacg
     2521 cttatctatc aagcgcaaac agctatgaat aatatagtaa aaaccgtggc tatcgtggac
     2581 tacggcatgg gcaatctgcg ctccgtctcc caggccgtgc aggccgcggc ccagggcagc
     2641 ggctggcatg tcgccgtcac cgccgatcct gatgtggtgc acgccgccgc ccgcgtggtg
     2701 ctgcccggcc agggcgccat gcccgactgc atgcgcgagc tgcgcgaatc cggcctgctc
     2761 gaatcggtgc tggaggccgc ggccaccaag ccgctgttcg gcgtctgcgt gggcatgcag
     2821 atgctgctgg accacagcgc cgagggcgac gtcgccggcc tgggcctgat cccgggcgag
     2881 gtgctcaagt tcgacctggc cggccgcacc caggccgacg gcagccgctt caaggtgccg
     2941 cagatgggct ggaaccgcgt gcgtcagatg cgccatggcg gccagctgca tgccatgtgg
     3001 gccggcgtgc cggacgacag ctacttctac tttgtgcaca gcttctacgc gcgcccggcc
     3061 aacccggcgc attgcgcggg ccaggccgac tacggcgcgc cctttgccgc ggccatcgcg
     3121 cgcgataata tttttgccac ccagttccac ccggaaaaga gtgccgaaca tgggttggcg
     3181 ctgtaccgca acttcctgca ctggaagccc tgactgactg gtggtgcgtt gtgggttgag
     3241 cgttttgcga tacccacatt gcatatcgct caaccttcaa cgctcaatct ctcccagacc
     3301 atgctgctca tccccgccat cgacctcaag gacggccact gcgtacgcct caagcaaggt
     3361 gacatggacc aatcgaccac ctttggcgaa gaccccgccg ccatggcgcg caaatggctg
     3421 gatgccggcg cacgccgcct gcacctggtg gacctgaacg gcgcctttgc cggccagccc
     3481 aagaacaagg ccgccatcaa gtccatcctg gccgaggtgg gcgcggacat cccggtgcag
     3541 ctgggcggcg gcatacgcga cctggacacg attgagcgct acatcgatgc cgggctggag
     3601 tacgtcatca tcggcaccgc tgcggtgaag aacccgggct ttctgaagga cgcctgcagc
     3661 gcctttgccg gccacatcat cgtcggcctg gacgccaagg acggcaaggt ggcgaccgat
     3721 ggctggagca agctgaccgg ccacgacgtc gtcgatatgg cgaagaagtt cgaggactgg
     3781 ggcgtcgaat ccatcatcta caccgacatc ggccgcgacg gcatgctctc gggcatcaac
     3841 atcgaggcca cggtgcggct ggcgcaggcg ctgtccatcc ccgtcatcgc ctcgggcggc
     3901 ctggccggca tcgctgacat cgagaaactc tgcgaggtcg agatcgaggg ggtggagggt
     3961 gtcatctgcg ggcgcgccat ctactcgggc gatctggact ttgcctccgc ccaggcgcgc
     4021 gccgacgaat tggccggggc ctgatgcgtc agcagctcgg cagggcgcgg cccctgttga
     4081 gcgcatccac cgtgtccagc atcacagtcc agtaacgggg cagcgccgcc cacgggttcc
     4141 cggtgttgct tgcgtagtcg tagtgcaggt cggtggcgct ttcggcctgc gaagttgcgg
     4201 tttccagtag aaatcaaaaa actctcagtc tctgagtcag cagtcacctt cggcgtggca
     4261 gtgacgggag ttgacagccg cgtcagcgac aatattcgcg agtagtgcaa cccaaggagg
     4321 gtgttttgat cctggtgacc ggaggcgcgg gctatatcgg ctcgcacatg tgtgtggcgc
     4381 tggcgcaggc gaacgagtcc tgcctggtgc tggacaactt cagtaacacc cgcccgtcgg
     4441 tgctggagcg cattgggcgc atcaccgggc aggcgcccga gtgcgtggag ggcgacatac
     4501 gcgatgcagc cttgctgaag cagttgtttg cgaacaacaa aatctctgcc gtcatccatt
     4561 tcgccgcgct caaggcggtg ggtgagtcgg tgcgcgagcc cctgcgctat tacgagaaca
     4621 acgtcgccgg taccgtgacc ctgctgcagg ccatgcagcg ggccggtgtg cgccagttgg
     4681 tgttctcgtc ctcggccacg gtgtatggcg accccgccag cttgcccatt cgcgaggact
     4741 tcccgctctc ggccaccaac ccctatggct ggagcaagct gatgatggag cagatgctgg
     4801 ccgacctgga cgccagcgag ccaggccagt ggcgcattgc gcgcctgcgc tacttcaacc
     4861 ccgtgggcgc gcacgagagc ggtctgatcg gagaagaccc gcagggcgtg cccaacaacc
     4921 tcctgcccta tgtggcgcag gtggccagcg gccagcgcga gttcctgaac gtctggggca
     4981 atgactactc cacgcccgac ggcacgggcc agcgcgacta catccatgtc tgcgacctgg
     5041 ccgagggcca tgtggcggcg ctgcgctacc tgcgccagca cccgggcctg ctgacggtga
     5101 acctgggcac gggccggccg gtgtcggtgc tggagatggt gcgcggcttc gaggccgcca
     5161 gcggtcgccc ggtgcgctac cagatcgggc cgcgccgcgc cggcgacgtg gccgcctgct
     5221 gggccgaccc cggcctggca gagcgcctct tgggctggcg cgcccggcgc gatgtgcagg
     5281 ccatgtgcca ggacgcctgg cgctggcagg acggcgtggc gcgtcagctg cagggcgcct
     5341 gagtcgtgct atcttgcccg ccccagtcgt gaaaccattg ccatgcttgc caaacgcatc
     5401 attccctgcc tggacgtgac cggtggtcgc gtcgtcaagg gcgtcaactt tgtcgaactg
     5461 cgcgacgccg gcgacccggt ggagatcgcc gcgcgctaca acgagcaggg cgctgacgag
     5521 ctcaccttcc tcgacatcac cgccaccagc gacgggcgcg acctgatcct gcccatcatc
     5581 gaggccgtgg ccagccaggt cttcattccg ctcacggtgg gtggcggcgt gcgcaccgtg
     5641 caggatgtgc ggcgcctctt gaacgccggg gcggacaaga ccagcttcaa ctcggccgcc
     5701 atcgccaacc ccgaggtcat caaccaggcg agcgacaaat acggcgcgca gtgcatcgtg
     5761 gtggccatag acgccaagcg ccgccagggt caggacctgg ccgagcgcgg cgcgggctgg
     5821 gacgtgtaca gccacggcgg gcgccagaac accgggctgg acgccgtgcg ctgggccgtg
     5881 gacatggcgc gccgcggcgc gggcgagatc ctcttgacca gcatggaccg cgacggcacc
     5941 aagagcggct ttgacctgca gctgacgcgc gccgtgagcg acgccgtcga cgtgtccgtg
     6001 atcgcctcgg gcggcgtggg caacctggat cacctggccg atggcgtgag catcggcggg
     6061 gccgatgccg tgctggcggc gagcatcttt cactacggcg agttcaccgt gcgccaggcc
     6121 aaggagcata tgcacgcgcg cggcattccc gtgcggcttt aaactattcc aatgaaatat
     6181 ggctgtagca cttatgtatc aagcgcaaaa agctataaaa ataatagtaa tactggtgcc
     6241 gtgccaaagc acgacaatgg cgtcatgaac tggcttgatc aagtgaaatg ggacgcgcag
     6301 ggtctggtgc ccgtgatcgc gcaggaggcc gccaccgggg acgtgctgat gttcgcctgg
     6361 atgaaccgcg aggccctggc caggacggcc gagctgggcc gcgccgtgta ctacagccgc
     6421 tcgcgcggca ggctttggtt caagggcgaa gaatcaggcc atgtgcagca ggtgcacgag
     6481 atgcgcctcg attgcgacaa cgacgtggtg ctgctcaagg tcacgcagct gggccatgag
     6541 cctggcatcg cctgccacac cgggcggcac agctgcttct tcagtgtcct caaggacggt
     6601 gcctggcagg ccgtcgatcc ggtcttgaaa gaccccgagt ccatctacaa gtaaagccca
     6661 tgtccagcac tgactccctc gcccgcctgg ccgccgtgat cgagagccgc aagagcgcca
     6721 atggcggcga cccgcaggcc agctatgtcg cgcgcctgct gcacaagggg cccgatgcct
     6781 tcctgaagaa gatcggcgag gaggccacag aggtggtcat ggccgccaag gacgtggatc
     6841 acggcgccgc acccgccaag ctggtgtatg aggtggccga cctgtggttt cactccatga
     6901 ttgccctggc ccactacggc ctcgccccgg ccgacgtgat cgccgagctc gagcgccgcg
     6961 agggcaccag cggcatcgag gaaaaggcgc tgcgcaaggt ggcgggccgc gccctggaag
     7021 aaggagaggc accgtgagca acgacatcat cgacatggag cccgacgagc gggcggagag
     7081 cctcaagacc ataggctggg tgagctacat cctgcacctg atcgtggccg tgggcgccgt
     7141 catgcccggc gcccagcccg gcgtggcgct gctcatcatc gcgctggtca tcgacctggt
     7201 gaaaaagggc gatgccgagg ggacctggca ggccagtcat ttctcctggc gcatcagcac
     7261 cgtgctctgg gccggcgtgc tgtatgtggt gacggcgccg ctgtggctgt tcttcgtgtt
     7321 cccgggctgg gtggcctggg ggttgatctc gatatggttc ctgtaccgca tcgtgcgcgg
     7381 catggtggcc atgaacaagg gccaggccat cgatgtctga agtcgcagcc acgccaccgc
     7441 tgaatctggc gctgcagggc ggcggctcgc atggggcgct gacctggggc gtgctggacg
     7501 cgctgcttga agacggcggt tttaccttcg agggcatcag cggtaccagc gccggcgcca
     7561 tgaatgccgt ggccctggcc catggctttg cccaggcagc gttgcagcac aaggatgccc
     7621 atgaggcgca ccaggccggc tgcgccctcg cgcgccagac gctcgcgcag ctgtgggaag
     7681 gcgtgggcac catgggcagc ctgctgtggg gcgtgccgct gcagggcaac ccgttcctgg
     7741 gcatgctgag ccagtggctc tcgccctacc agaccaatcc gctgggcatc aacccgctgc
     7801 gcggcctgct cgagcgcgtg gtggacttcg acgccctgtg ccatgcgcgc catgcgctgg
     7861 tgcccaaggt gttcgtctgc gccaccaatg tgcgcacggg acgcggcgag atcttctcgg
     7921 gcgcgcgcct gtcggccgac gccgtcatgg cctcggcctg cctgccgctt ttgttcaagg
     7981 cggtgcagat cgatggcgag cattactggg atggcggctt ctccggcaac ccggcgctgt
     8041 acccgctgat ctaccagacg caatgcgccg acgtgctgct ggtgcagatc aacccgatag
     8101 aacaccctga cctgcccgat accgcgccgg agatcatgga gcgcatgaac gaggtcacct
     8161 tcaacgccag cctgctgggc gagttgcgcg ccatcgactt cgtgcgccgc ctgctggccg
     8221 aggggcggct cgatccccag cactacaaga gcatgcgcat gcaccgcatc gatggcggcc
     8281 gcgtgctggc gccgtttggc gacgccagca agtcacgcgc cgacatggcc tttgtgcgcc
     8341 agctgttcga gctggggcgc acccagggca agcaatggct gcgacgccac cgccgcgacg
     8401 tgggcgtgca gcacacattg catttgaccg acaatcactg actgttttcg ctgctttgct
     8461 tcatagccgc tccatgcacg accccaactg ccttttctgc aagatcattg ccggccagat
     8521 cccgtcgaag aaggtctacg aggacgaaca ggtcttcgcc tttcatgaca tacacccctg
     8581 ggcgccggtg cattttctca tcgtgcccaa gctgcacctg ccttccatgg cgtccgccac
     8641 gcccgagcat gccggcctgc tcggccacat gatgctgctg gcccccagac tggccatcga
     8701 gcagggctgc aacccctatc cggacggggg ctaccgcatc gtggtgaaca ccggcaccga
     8761 gggcggccag gaggtgcacc acctgcacat gcatgtgatc ggtggcccgc gcccctggtt
     8821 gcgcggttga cctgcagcac agggcagccg cacgggttaa gccggttgcg actggggcgg
     8881 gtctaaaatg tgaggcattc gttaggagat ttccatgggt tcgttttcca tttggcactg
     8941 gctcatcgtg ctgctgatcg tggtcatggt gttcggcacc aagaagctca agaacattgg
     9001 ctccgacctg ggcggcgccg tgaagggctt caaggacggc atgaaggagg gcgccagcag
     9061 tgatgagcag gcgggtgcca acgcgcctgc cggccaggtc accaacacca cggccgccga
     9121 caagaccacc atcgacgtgc aagccaagca aaagagctga gcgcgccggc tgagcgtttt
     9181 tcatgatcga cattggcctg tccaagatgg cgctgatagg cgccgtggct ctcatcgtca
     9241 tcggcccgga gaagctgccg cgcgtggcgc gcaccgtcgg caccctgctg ggtaaggcgc
     9301 agcgctatgt gtccgacgtc aaggccgagg tcaaccgttc catggagctc gacgagctgc
     9361 gcaagatgaa ggatacggtg gagggtgccg cgcgcgacgt ggagcaatcc atacagacca
     9421 gcgccagcga cttccagaag gacatggaaa gcagcctggg tgaagacttc gccccgctgg
     9481 gcgagccatc cggcgcgcaa gagctgtcct ccgtggttcc tgcctacaag cacccgggca
     9541 agaactggcg cctcaagcgc ggcgccatgc cccagtggta caaggcgcgt gccggtgtgc
     9601 gcaccaaggc ccagtcgggt gcggcgcgcg tggcgcgctt tcggccccaa aaattccatt
     9661 gacccccttt gaatggctgc accgcccgtc gtgcagcctg ggccggcgcc aagccgacca
     9721 cctgccccgc catgcctgaa acaccgaacc ctgaagacga actcgccggt accgagcagc
     9781 ccttcgtgca gcacctgatg gagctgcgcg accgcctggt caaggccatg atcgccatcg
     9841 gggttgtcgc cgccgcgctg ttcttctacc ccggcccggg ccagctctac gacctgctgg
     9901 ccgcgccgct ggtggcgcac ctgccccagg gcgccaccat gatcgccacc tcggtgatct
     9961 cgcccttcat ggtgcccttg aagattctgc tgatgtcggc ctttctgatc gctctgccct
    10021 ttgtgctatg gcaggtctgg gcctttgttg cgccgggtct gtatgcgcat gagaagcgcc
    10081 tggtgctgcc gctggtcgtc tccagcaccg tgctgttctt catcggcgtg gcgttttgct
    10141 actttttcgt ctttggccag gtgttcagct tcatccaggg ctttgcgccc aagagcatca
    10201 ccgccgcgcc agacatcgaa gcctacctgg gctttgtcat gaccatgttc ctggccttcg
    10261 gcctggcgtt cgaggtaccc attgccgtgg tcgtgctggc gcgcatgggc gtggtcagcg
    10321 tggaaaagct caagagcttt cgcggctact tcatcgtcgt cgccttcgtg atcgccgcca
    10381 tcgtcacgcc gcccgacgta gtgtcgcagc tggcgctggc gattcccatg tgcctgctgt
    10441 acgaggtggg catctgggcg gcgcaggtct tcatccgcca cacgcaggcg cccgaggaat
    10501 cgcaggagtc cacctcctga tattgatgaa aatagctgtt tgcgcccgca catcaagcgc
    10561 aggcagctat taattcaata gtaaatttca gcggcgctgc tgcatgcgcg ggcgcgtgcc
    10621 cggcaccacc gacagctcca gctgcctgtc gccgcgctgc agctcgaagc tggcggcctt
    10681 gcccggctgc aggccggcca cggcggtgag cagttgcgag acattgtcgg tgggcttgcc
    10741 ggccacgctc aggatcacgt cgccggggcg tatgccgcct tgcgccgcgg gcgcgccctg
    10801 caacacgccg gtgatgatca cgccccgcgt ggcctgcacg ccaaaggtct gggccagctc
    10861 cggtgacagc tcgcccggct ccacgcctat ccagccgcgc gtcacctgac cgtcacgcac
    10921 gatgccgtcg agcaccagct tggccgtgga cacggggatg gcaaagccaa tgcccatgct
    10981 gccgcccgag cgcgagtaga tggcggtgtt gatgcccatc aggttgccat tcacatccac
    11041 cagcgcgccg ccggagttgc cagggttgat ggccgcgtcg gtctggatga agttctcgaa
    11101 ggtgttgatg cccagctggt tgcgccccag cgccgagacg atgccgctgg tgaccgtctg
    11161 ccccacgcca aaggggttgc cgatggccag cacctggtcg ccgacgtcga gcttgtccga
    11221 gtcgcccagc acgatcaccg gcagcttgtc cagctgaacc ttgagcacgg ccaggtcggt
    11281 gtcggggtcc gtgcctatca ccgtggcgct ggcgcggcgg ctgtcggtga gcgtgacctc
    11341 gatctcgtcg gcgccctcga ccacatggtt gttggtcagg atgtagccct cggggctgat
    11401 gatcacgcca ctgcccaggc cggtttgcgc ctgcgcgccc tggtcgccaa agaagaactg
    11461 gaaccagggg tcgttgctgc gcgggtggcg cacctccttg ctggtattga tgctgaccac
    11521 ggccggagcc gccttgcgcg ccgcggcact gaagctgccg gccgcgggct gggcgcccgt
    11581 actgggcggc gcctcgatga gggaaatgcc ggcaccgctg gtggtcgcac cgcgttgcag
    11641 ccagccgggc tgcagcgtgg cgacgacgaa ataggcggcg gcgagcaccg tcacggcttg
    11701 ggaaaacagc aaccaatagc gtttcatgat gcgggggagg gcggccctgg tggggaaaag
    11761 gcaattgtcg cgtgtttgcc gcaggcactg gcggtgcttg ggcacaatcg gcggcatgag
    11821 catccagcga tctgaattac tggcgcattt cgacgcgctg ctgcagcccg agcgcttcaa
    11881 ggactatggc cccaacggcc tgcaggtgga gggtgcgcag acgatacggc gcatcatcag
    11941 cggcgtcacc gccagccgcg cgctgatcga tgcggccata gcggcgcgcg ccgacgccat
    12001 cttcgtgcac cacggcctgt tctggcgtgg catggacggg cgcgtcacgg gctggatgaa
    12061 gcagcgcctg cagcgcctgc tggcgcatga catcaacctg tttgcctacc acctgccgct
    12121 ggacgcccac cctgagctgg gcaacaacgc gcagctgggc cgggtgctgg gctggcaggc
    12181 cgatcaaagc tttggcgagc aggatctggg ttttgccgcg ccggccgcat tcgccagcgc
    12241 cgctgaactg gcagcccatg tgcaggcgac gctggcccgg ggcgtgacac tggtggcgcc
    12301 cgagggcgcg caccccatgc gccgcgtggc ctggtgtacg ggcggggcgc agggtttttt
    12361 cgaatcggcc attgccgccg gggcggatgc cttcatcacc ggcgagatct ccgagcccca
    12421 gacgcacctg gcgcgcgaga ccggcgtggc cttcatcgcc gccggccacc acgccacgga
    12481 gcgttatggc gcaccggccg tcgcggcgca tgtggcgcag cagctgggcc tggagcacca
    12541 gttcatcgac attgataatc cagcctgatt attttgatag cgtctcacgc tttatgggtg
    12601 ggcgctagag cctgatttgg tatatttttt gatgagcaca tctctcccac tggccattac
    12661 ccagggcgac ccggccggca taggcccgga gatcgtcgcc aaggcctttc gcgacgcacc
    12721 ccgggacatg cgtggctgct ttgtcgttgg cgacctggcc acgctgcgcc gtgccgcggc
    12781 ctgcatcgag cggccgggcg acaccagcct gccgctggcg cgcatcgacg ccctgcagga
    12841 tgtggcgctg gtgccgccgc gctgcctgcc ggtgtggcag ctgccagagc tggctggcgg
    12901 cgaacgtgag ccatggggca gggtgagcgc cggtgccggg cgcgccgctg cggcctgcgt
    12961 gctctgggcg gcacgggcgg cgcttgccgg ccaggtcgcg gcgctggtga cggcgccact
    13021 gcacaaggaa tcgctggcag ccgcgggcgt gccgtttccg ggccacaccg agctgctgca
    13081 ggccgaggcg gcgcgtcatg ccggcgtgcc cattgcgcag atgccggtgc gcatgatgct
    13141 ggccagcgac gagctgcgca cggtgctggt cagcatccac atgtcgctgc gcgaggccat
    13201 tgccgccgtg acgccggaca acctgctgca gacgctgcgc atcacccatg ctgccctgtc
    13261 acgcagcctg ggacgggcgc cgcgcatcgg ggtggcgggg ctcaaccccc atgcgggtga
    13321 gggcggcctg tttgggcgcg aggagctgga catcatcgcc ccggccattg cccaggcgcg
    13381 cgccgagggc atggacgccc atggccccta tgcgcccgac accatcttca tgcgcgcgcg
    13441 ctgtacggcc ggcaggccgg gcgagttcga cgtggtgctg gccatgtacc acgaccaggg
    13501 cttgatcccc gtgaaatacc tgggcgtgga caagggcgtg aacgtcaccc tgggcctgcc
    13561 cctggtgcgc accagcccgg accatggcac ggcctttgac atcgcgggac ggggcgtggc
    13621 cgatgccgcc agcctgatcg aggccgtgcg catggcgcgt gcgctggcag gttgaacaat
    13681 cgtcaaaaag acaaacgccc gtggcaattg cgccacgggc gtttggtgcg agcagaaaaa
    13741 aatcagcgct tgaggctgtc gcggatctcg cgcagcagca cgatgtcttc cggcgtgacg
    13801 gcttcgggta cgggctcggc gggcgcctcg cgcttgaggc ggttgaactg tttgaccatc
    13861 atgaagatga tgaaggccag gatgatgaaa ttcaccgcca cggtgatgaa gctgccgtag
    13921 gcaaacaccg gcacgccggc cttcttgagc gcatccagcg tgcgcgccgt gccttcgggg
    13981 atgctgccca gcacgatgaa gaggttggag aaatcgagct tgccgaacac cagaccgacg
    14041 atgggcatga tcaggtcggc caccaccgaa tccacgatct tgccgaatgc ggcgccgatg
    14101 atcacaccca ctgccaggtc aatcacattc cccttgacgg caaactcttt gaattctttg
    14161 gtgatgctca ctttttacac ccttggatga tgttgaaaaa aaaccggaat cagtccggat
    14221 cgtcagctta agtgcgtatc ggtcgttgga gcgagcttta ccgctcggct acaatttacg
    14281 gttgactcca tagcgaagca taccgaggaa ccccatgagt gaaactccga tcgactcgag
    14341 caagcggacg tggctgatcg cgtctggctg cgctggcgcg gtgggtggcg tggccacagc
    14401 cgtccctttt gtgagcagct tccagccgtc cgagaaggcc aaggccgccg gcgcggccgt
    14461 cgaggtggac atctcggccc tcaagcccgg tgaaaaggtc acggtggaat ggcgtggcaa
    14521 gccggtgtgg atcattcgcc gcacgcccga gcaggttgcc gaactgccca agctcgacag
    14581 ccagctggcc gacccgcagt ccaagcgcaa tccggccgag ttcacgccgc cgtatgcgca
    14641 gaacgagcac cgctcgatca agcccgagat cctggttgcc gtgggtatct gcccgcacct
    14701 tggctgctcg ccctccgaca ggttcacccc cggcccccag ccgtcgctgc ccaatgactg
    14761 ggagggcggt tttctgtgcc cttgccacgg atcgaccttc gacatggccg gccgcgtgtt
    14821 caagaacaag ccgtcgccgg acaacctgcc ggtgcccccg cacatgtacc tgtcggacac
    14881 caagctcctg atcggtgaag acaagaaggc ctgagggagg cactgacaaa tggctgctta
    14941 ccgcgaattc aaggaaatct cgcccaacgc ctcggcgacg gcgaagacaa tgaactggct
    15001 ggagaaccgt tttccaacgg ccttcgacgc ctacaaggtt cacatgtcgg agtactacgc
    15061 tccgaagaac ttcaacttct ggtacatctt cggctcgctg gc
//
LOCUS       DAOMTQ010000066        15024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_630_length_15024_cov_26.974881, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000066 DAOMTQ010000000
VERSION     DAOMTQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_630_length_15024_cov_26.974881"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1145
                     /locus_tag="PK518_08205"
     CDS             120..1145
                     /locus_tag="PK518_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HRM48936.1"
                     /translation="MTQDAGPTSDPVSLPSQQPKLLDRLRTHLRTRHYSIRTEEAYID
                     WARRFILFHGKRHPQDMGAAEVEAFLSHLAVDRQVSASTQNQAKAALLYLYKQVLAID
                     LPWLDEVIQAKRPRRLPVVLTPSEVRALLLHMDGTTGLIAQLLYGTGMRLMEALRLRV
                     KDVEFERREIVIREGKGNKDRITVLPENMIASLKTQLQKAHALHEKDFAAGLGRVFLP
                     HALAVKYPQADKNWAWQWVFPSPVRSIDPRPDARTGQNIERRHHVYPESVQRAVREAA
                     RLANINKPVSPHVLRHSFATHLLQAGYDIRTVQELLGHADVSTTMIYTHVLNKGGRGI
                     LSPLDAL"
     gene            1176..2564
                     /locus_tag="PK518_08210"
     CDS             1176..2564
                     /locus_tag="PK518_08210"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRM48937.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAARLFVQRLARGGRMAQVA
                     RVRSTLYGSLGATGRGHASDRAVLLGLCGHAPDTVDVALIDPLIARIRGQHRLPLEGG
                     PEIAFDWNADLPLIADESLPLHANGMRFEAFDAAGNLLDAQVYYSVGGGFIVSEEAAQ
                     DAQRQAQIAPDTEVLPLPFHSGAQLLAQAQAQGGSIAQVMRTNERHWRQDAEIDAGLL
                     AIWRVMQDCVARGCGAEGELPGGFRVRRRAPALHRALQASQSLQQDPLQVIDWVNLFA
                     LAVNEENAAGGRVVTAPTNGAAGIVPAVLHYYWRYIDGATEAGVIDFLLTAGAIGILY
                     KENASISGAEVGCQGEVGVACSMAAAGLCAVLGGTPAQVENAAEIGMEHHLGLTCDPV
                     GGLVQIPCIERNALASVKAINAARMALHGDGTHHVSLDQVIKTMRETGADMMTKYKET
                     SRGGLAVNIVEC"
     assembly_gap    2811..2910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3138..4706
                     /locus_tag="PK518_08215"
     CDS             3138..4706
                     /locus_tag="PK518_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRM48938.1"
                     /translation="MHPDIEHLLSQPFGSLPELIARQAALRPGHKAVILEGQSLDYAA
                     LCAGMNRVAASLQRDGLGPGDVLAICAGASIEYVLAYLGALRAGVAVAPLAPSATAGH
                     LSAMLDNCGAHLVLRDREVAAQWPLHEGAALHCVALDDVPEAGQPWSQWLAAGDAAPD
                     AITPRPDWPFNVIYSSGTTGVPKGIVQSWAMRWAHVQRAVGNGYGPDAVSLCATPLYS
                     NTTLVAALPTLALGGTLVLMRKFDAAHYLRLAEQHGATHSMLVPVQYQRLLACPTFDT
                     TDLSRLQNKFCTSAPFSAKLKAEVLRRWPGRLIEYYGLTEGGVRCELHCHDFPNKLHT
                     VGRPADGADMRFIDEQGRELPAGEQGEIVGRSAGMMSGYYRLPEKTAEVEWFDGEGNR
                     FIRSGDVGRLDEDGFIVLGDRMKDMIISGGFNIYPSDIEAVLAQHPQVAECAVIGVPS
                     EQWGETPVAYVVARAGAQPDAAELRVWLNERVGKTQRVADLRIAASLPRSEIGKVLKR
                     ALRDQYVQAPPAST"
     gene            4865..5857
                     /locus_tag="PK518_08220"
     CDS             4865..5857
                     /locus_tag="PK518_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48939.1"
                     /translation="MPMYFSRLRRTLVASLLLPAAALLAPAAHAADPWPTKPIQLVIP
                     YPPGGSADLLGRPLAVQLQQQLGQPVVLEYKPGAGGTLATQYLARAKPDGYTVLMVLA
                     AHAINDSLYPKLPYDTRKDFAPVSWVANLPMVVAGSSKLPAKNIQELIQAAKAAPDKL
                     TFGSAGNGNTGHLAAEYFSSIAGIKMTHVPYKGSAGVVNAMLAGDIDLTFDSISTSMP
                     HIRSGRMHALAVTSAQRSALAPDVATVQEQGVAGFDVTGWYALIAPAGTPSEVTQRLS
                     SEIASALRQPQLQTQLAAGGYVPVGSTPEALQAHIEREITRWAAVVKSTGAKVD"
     gene            5981..6358
                     /locus_tag="PK518_08225"
     CDS             5981..6358
                     /locus_tag="PK518_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRM48940.1"
                     /translation="MQRAATTPQPDPAAVLAKATARASELLGLTGVALARTIGLSEPT
                     VSRILRGEKTIAPTSKEGELALLLVRVYRSLDALVGTDDARRRAWMDTYNQALGGRPL
                     QLVQRADGLVATLNYLDGMRAPA"
     gene            6358..7068
                     /locus_tag="PK518_08230"
     CDS             6358..7068
                     /locus_tag="PK518_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRM48941.1"
                     /translation="MTLAADWERFWLEPGIGAQSMLAWRGVEAQHVVSTMRLVDSAAE
                     QDLLEQLLEASKPALPATRQPRHYLLLTPFRYRPHHPSRFRPAHARGQWYGAEALYAA
                     CAEVAYWRRRFILDSTGLQDQTLLTEHSFFQAAIDGQAMDLMAPPWNQARALWTQGDD
                     YAATQAVAAGAQQRGVQWLAYESARASGQRCAVVFDPDCLAEPAGGLDHTLQTWHCKA
                     TRASVMFTRGRERHEWHF"
     gene            7095..7541
                     /locus_tag="PK518_08235"
     CDS             7095..7541
                     /locus_tag="PK518_08235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRM48942.1"
                     /translation="MSSNDIPLSPFAGEAPGLLFGLPMPMSRAMALRGERIGNDRAQV
                     RMGFQAEQSNSRGDVHGGAIASLLDCTLAGAARAHDPAAYGVVTIDLTLHFVSAASGD
                     LIATAHCERRGRSISFVRGEVRAEDGTLVALATGTFKLVARQPARG"
     gene            complement(7594..8604)
                     /locus_tag="PK518_08240"
     CDS             complement(7594..8604)
                     /locus_tag="PK518_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48943.1"
                     /translation="MNKPMQPPTCNRRQLLALAAGAAAAGPLAARAQTGFATRPVKVV
                     VPFAAGGATDVITRVLGEHMGKRFGQPVVVDNKPGGAGLIAGEMVVKSPPDGMTVLLG
                     TTSSMLTNKYLYKKPPYDPLADLTPLVRVCLAPIALVVTNDVPAQNLQEFMAWLRANK
                     GKLSYGSYGIGSHGQLACTTLSDVAGADMAHVAYKGEAPMIQDLLGGQIKIGMGSLLS
                     IKAHIDSGRLRALAVTGPRRVPLLPDVPTFAEAGLKQEALALVGWLAIAGPKGMGADV
                     ARQWAQAANQAVASREGMARIIAAGFVPVDDDTPESFGRLWAQEGPIWGRLLQAAGVQ
                     PN"
     gene            complement(8728..9708)
                     /locus_tag="PK518_08245"
     CDS             complement(8728..9708)
                     /locus_tag="PK518_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM48944.1"
                     /translation="MTRNRRTTLALIALAACATLGTSSAQAQGDYPAKPLRFIVPYPA
                     GGGTDTIARLIGTQLTQRWGQPVVVENKPGASGILGNDIVAKAPGDGYTVLMGITAVV
                     QIPALYKKVPYKLADLAPVSQIAKSADLLMVPRSSGVTTLAQFVDKAKAAPGTMNYGT
                     YGNATSSHMNGERFKQQAGIDLTHIPYQGSGPEMAALLGGQLTLAFVDATAAYPHIKS
                     DKLNILAVTGSQRFPSLPNVPTMAESGYPGLEANGWFGVFLPASTPKAIVDKLGAEVA
                     AIVKSPELNKRLTDMGLIAVGSLPDDFKTQVEKDAAHWKAVAEGAKISMD"
     gene            complement(9733..10875)
                     /locus_tag="PK518_08250"
     CDS             complement(9733..10875)
                     /locus_tag="PK518_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM48945.1"
                     /translation="MQDLTTRSIYREDHEQFREQARRFFEREVVPHHAQWERDGIVPK
                     EVWRKAGREGLLNPMLPEPYGGGGDFGHAAVLLEEIARAGASGLGFPLHSDIVAPYIF
                     AYGNKEQKDRWLPRMAAGELIGAIAMTEPGAGSDLKSVRTTAVRDGDHYVLNGSKTFI
                     TNGINSEIVIVVAKTAPELGAKGVSLIVVEDGTPGFSKGRKLEKIGLMAQDTSELFFD
                     NVRVPVGNLLGEENLGFRYLMQELAQERLVVAVRAAASVETFLQKTVDYTRERKAFGQ
                     TVFEFQNTRFKLAEAKAQATMLRVFVDDCIALHLRRELTPERAAMVKLNATVLQNRLL
                     DEFLQLHGGYGYMTEYQVGRAWTDARIGRIYGGSDEIMKEIIARTL"
     gene            complement(11177..12736)
                     /locus_tag="PK518_08255"
     CDS             complement(11177..12736)
                     /locus_tag="PK518_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM48946.1"
                     /translation="MQDFFGRAKAAPDRVVVVLAEQGRSCTAGALAQQALQMAQWLAA
                     QGLQPGERFAVLLENRAEILALVLAAREAGVYAAVLSTHLTADEVAYIVQDCDARLIV
                     ASSQTLPQLADLPRTLALPCWTVDEAQPQAASLPAALAALPGPAIDLSDRPLGRDLLY
                     SSGTTGRPKGVLKPLWPASLRGQCDPEALGTARLMQVDERTVYLSPAPLYHAAPLRYT
                     LRVLELGGSAVIMERFDAQSALELIERHRVTHSQWVPTMFTRMLRLPEELRRRHDLSS
                     HRVAIHAAAPCPVDVKRAMLDWWGDILMEYYAGSEGCGTTMINSAEWRLRPGSVGRAT
                     TGQLHIVDDQGRELPAGEIGQVFFSGGGTFSYLNDPEKTRQALNEQGWFTYGDIGHVD
                     ADGYLFLSDRRADLILSGGVNLYPQEIENALARHPDVQELAVVGVPHPDFGEVPLAAV
                     VLRPGAEPSPATARAIAGQAAVVLARLKLPQRMVFVESLPRLETGKLLRRKLKEQYRD
                     APQAGFALRDS"
     gene            12946..14124
                     /locus_tag="PK518_08260"
     CDS             12946..14124
                     /locus_tag="PK518_08260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRM48947.1"
                     /translation="MKNPVIVDAIRSPMARAKPDGALAQLHPVDLLSQVLQQLVERNK
                     IDPGQVDDVICGCVSQAGEQAGTPGRLAWLAAGLPTHVPSTTIDRKCGSSQQAVHFAA
                     QAIMAGVQDMVIACGVESMSRVPMGSSRMGQNTTGARFDARYAPGLVGQGVSADLVAA
                     KWGLSRTELDAYAARSHQRAAAAQSAGRFEREIVAIDTPAGRVLADETIRAGTTVEKL
                     AGLKAVFESEELSKRFPQIQWSTTAGNASQMTDGASAMLIMSEERALQLGLKPRARFV
                     AFDVVGDDPLYMLTAPIPATQRVLAKAKMKLEDIHHYEINEAFASVPMAWQKELQADG
                     ERLNPRGGAIALGHPLGASGIRLMTTMLHALEDSGQRYGLQSMCEAGGMANATIIERL
                     "
     gene            14273..>15024
                     /locus_tag="PK518_08265"
     CDS             14273..>15024
                     /locus_tag="PK518_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM48948.1"
                     /translation="MKLQPGMTALVTGAVSGLGEASAIALLERGLNVVAVDLNDERGA
                     AMEAKYPGRLRYVKADVSDEAQMQAAVAAAEAFGNFRALVHCAGIGGPLRLIEKDGSA
                     GSLEKYTNIIRVNQIGTFNTLRLAAVAMAKNEPVDGERGACVLTASIAGYEGQIGQIP
                     YASSKAGVIGMTIVAARDLATKLIRVCTIAPGLFDTPILAKLPENVRQSLGASVPHPA
                     RLGHPSEYALTALHILENPMLNGETIRLDGAIR"
BASE COUNT         2395 a         5007 c         5059 g         2463 t
ORIGIN      
        1 ctaactagaa ttagacgact gcgatcttag cggtggatgt cggaatgtgc aatacgcttc
       61 gtaacgtcta caggcaaacc ccatcgtgcg gctgtttagt caaacctaaa attttgcgga
      121 tgacacagga tgccggcccc acttctgatc ccgtctcgct gccgtcccaa cagcccaagc
      181 tgctggatcg cctgcgcacc cacctgcgca cgcggcacta cagcatccgt accgaagagg
      241 cgtacataga ctgggcacgg cgcttcatcc tgtttcatgg caagcgccac ccgcaggaca
      301 tgggcgcggc cgaggtagag gctttcttga gccatctggc ggtggatagg caggtgtcgg
      361 catccacgca gaaccaggcc aaggcggcat tgctttattt atacaaacaa gtgcttgcta
      421 tcgacttgcc ctggctcgac gaggtaatcc aggccaagcg cccgcgacgc ttgccggtgg
      481 tactcacacc atcggaggtg cgcgccttgc tgctgcacat ggatggcacg acgggcctga
      541 ttgcccaact gctgtacggt accggcatgc ggctgatgga ggccttgcgc ctgcgcgtga
      601 aagacgtgga gtttgagcgg cgcgagattg tgattcgaga gggcaagggc aataaagacc
      661 gaataacggt attgccagaa aatatgattg cctccttgaa aacccaattg caaaaggccc
      721 acgccttgca tgaaaaggat tttgccgctg ggctggggcg tgtgtttttg ccgcatgcgt
      781 tggccgtcaa atatccgcag gctgataaaa actgggcttg gcagtgggtt ttcccttcac
      841 ccgtgcgttc cattgatcca agacccgatg cacgcacggg ccagaatatc gagcggcgcc
      901 accatgtgta ccccgaatcc gtgcagcgcg ccgtgcggga ggcggctagg ctggccaata
      961 tcaataaacc cgtctcgccc catgtgctgc gccattcgtt tgccacgcat ttgctgcagg
     1021 cgggttatga cattcgcacg gtgcaagagt tgctgggcca tgccgatgtg agcacgacca
     1081 tgatttacac ccatgtactc aacaagggcg ggcggggtat tttgagcccg ctggatgcgc
     1141 tgtagcattc cccgacatca gacaggaggt tggccatggc cgtcagcgtt ttcgaccttt
     1201 tcaagatcgg cataggcccg tccagctcgc acaccgtggg gcccatgcgc gcggcgcggc
     1261 tgtttgtgca gcgcctggcg cgcggcgggc gcatggcgca ggtggcgcgc gtgcgcagca
     1321 cgctgtatgg ctcgctgggc gcaacgggtc gggggcacgc cagcgaccgg gcggtgctgc
     1381 tgggtctgtg cggccatgcg ccggatacgg tggatgtggc cctcatcgat ccgctgatcg
     1441 cgcgcatccg cggccagcac cggctgccgc tggagggcgg ccccgagatt gccttcgact
     1501 ggaacgccga cctgccgctg attgccgacg aaagcctgcc gctgcacgcc aacggcatgc
     1561 gcttcgaagc cttcgatgcg gccggcaacc tgctggacgc gcaggtgtat tactcggtgg
     1621 gcggcggctt catcgtcagc gaagaggcgg cccaggacgc gcagcgccag gcgcagattg
     1681 cgccggatac cgaggtgctg ccgctgccgt ttcacagcgg cgcgcaacta ctggcgcagg
     1741 cgcaggccca gggcggaagc attgcccagg tgatgcgcac caacgaacgc cactggcgcc
     1801 aggacgccga gatcgacgcc ggcctgctgg ccatctggcg cgtgatgcag gactgcgtgg
     1861 cgcgcggctg cggcgccgag ggcgagctgc ccggcggctt tcgcgtgcgc cggcgcgccc
     1921 ccgcgctgca ccgggcgctg caggcgtcgc aaagcctgca gcaagacccg ctgcaggtga
     1981 tcgattgggt gaacctgttt gccctggcgg tcaacgaaga aaacgccgcc ggcgggcgcg
     2041 tggtgacggc gccgaccaac ggcgcggcgg gcatcgtgcc ggcggtgctg cattactact
     2101 ggcgttatat cgacggcgcg accgaggcgg gcgtgatcga ctttctgctc accgccggcg
     2161 ccatcggcat tctgtacaag gagaacgcct ccatctcggg cgccgaggtt ggttgccagg
     2221 gcgaggtggg cgtggcctgc tccatggccg ccgccggtct gtgcgccgtg ctgggcggca
     2281 cgcccgcgca ggtggagaac gccgccgaga ttggcatgga gcaccacctg ggcctgacct
     2341 gcgacccggt gggcgggctg gtgcaaatcc cctgcataga gcgcaacgcg ctggcatccg
     2401 tcaaggccat caacgccgcg cgcatggcgc tgcacggcga cggcacgcac catgtgagcc
     2461 tggaccaggt catcaagacc atgcgcgaga cgggcgccga catgatgacc aagtacaagg
     2521 agacttcgcg cggcgggctg gcggtgaata tcgtggagtg ctgaggcacg gcgcttatat
     2581 tttcatagcg cgttgcgctt tgagtatgcc ggttttggct gcttttccct ccccattcgt
     2641 gggggcagcg acgttcaccc cctcatgccg gcaagatgcc ggcgctcccg gcccccatcc
     2701 ccacctgccc cccagagggg gaaggagtct gagacagctt gctgatcaag catatttcta
     2761 tagcgcgctg tgctgtcatc gtgccgctgt tggctgtttc tccctctccc nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccctctcccc caggggggcg agggagttga
     2941 accccgtgcc aaaccagcgg tgtgcgctaa caaaaggtgt gtgcatgcac gcaaggcacg
     3001 cggcaacgta ttcccgcggg actggcagcc agagccacaa ccgccctttg agtgcgccaa
     3061 agccggggtt gcccaatcac gaaacccatg acacgctggc cgccttatcg tgacgcctcc
     3121 accgaccggc aagacccatg caccccgata tcgaacacct gctgtcccaa cccttcggct
     3181 cgctgcccga attgatcgcg cgccaggccg cgctgcggcc cggccacaag gctgtgattc
     3241 ttgaagggca aagcctggac tacgccgccc tgtgcgccgg gatgaaccgg gtggccgcca
     3301 gtctgcagcg tgacggcctg gggccgggcg atgtgctggc gatctgcgcc ggcgccagca
     3361 tcgaatatgt gctggcctac ctgggcgcgc tgcgcgccgg ggtggccgtg gcgccgctgg
     3421 cgccctcggc cacggccggg cacctgagcg ccatgctgga caactgcggc gcacacctgg
     3481 tgctgcgcga ccgcgaggtg gctgcgcagt ggccgctgca cgagggcgcc gcgctgcact
     3541 gcgtggcgct ggacgatgtg cccgaggccg gccagccctg gtcgcaatgg ctggccgcgg
     3601 gcgatgccgc gcccgacgcc atcacgcccc ggcccgactg gccgttcaac gtgatctatt
     3661 catccggcac cacgggcgtg cccaagggca tagtccagtc ctgggccatg cgctgggccc
     3721 atgtgcagcg tgccgtcggc aatggttatg ggcccgatgc cgtgtcgctg tgcgccacgc
     3781 cgctgtactc caacaccacg ctggtggcgg ccctgcccac gctggcgctg ggcggcacgc
     3841 tggtgctgat gcgcaagttc gacgccgcgc attacctgcg gctggccgag cagcatggcg
     3901 ccacgcacag catgctggtg cccgtgcagt accagcgcct gctggcctgc cccacgttcg
     3961 acaccacgga tctgagccgc ctgcaaaaca agttttgcac cagcgcgccg ttcagcgcca
     4021 agctcaaggc cgaggtgctg cgccgctggc cgggccggct catcgagtac tacggcctga
     4081 ccgagggcgg cgtgcgctgc gagctgcatt gccatgactt tccgaacaag ctgcacaccg
     4141 tcggccgccc cgccgatggc gccgacatgc gcttcattga cgaacagggg cgcgagctgc
     4201 ccgccggcga gcagggcgag atcgtcggac gctcggccgg catgatgagc ggctactacc
     4261 gcctgcccga gaagaccgcc gaggtcgaat ggttcgacgg cgagggcaac cgcttcatcc
     4321 gcagcggcga cgtgggccgg ctggacgagg acggcttcat cgtgctgggc gaccgcatga
     4381 aggacatgat catcagcggc ggtttcaaca tctaccccag cgacatcgag gccgtgctgg
     4441 cgcagcaccc gcaggtggcg gaatgcgcgg tgataggcgt gccctccgag caatggggcg
     4501 agacccccgt ggcttacgtg gtggcgcgcg cgggggcgca gcctgacgcc gccgagctgc
     4561 gcgtctggct caacgagcgc gtcggcaaga cccagcgcgt ggccgacctg cgcatcgccg
     4621 ccagcctgcc gcgcagcgag atcggcaagg tgctcaagcg cgccctgcgt gatcagtatg
     4681 tgcaggcacc gcctgcaagc acctgacgcg cgggccagga gcgccggcgt cccgccggca
     4741 gggcctgatt ttgctgccgc taccgccagc gggagaggca gcaccggtgc gcatgccttg
     4801 gtgccggcgg gacgccggcg ctcccagcct ttgtgccagt gaattttcaa aataggagac
     4861 cgccatgccg atgtatttct cgcgactgcg ccgcaccttg gtcgccagcc tgctgctgcc
     4921 cgccgccgcc ctgctggcgc ctgcagccca tgccgccgac ccctggccca ccaagcctat
     4981 ccagctggtg attccctacc cgcctggcgg cagcgcagac ctgctgggcc ggccgctggc
     5041 ggtgcagctg cagcagcagc tgggccagcc tgtggtgctg gaatacaagc ccggcgcggg
     5101 tggaacgctg gccacgcagt acctggcgcg cgccaagccc gatggctata ccgtgctgat
     5161 ggtgctggcc gcccatgcca tcaacgacag cctgtacccc aagctgccct acgacacacg
     5221 caaggacttc gcgcccgtat cctgggtggc caacctgccc atggtggtgg cgggtagcag
     5281 caagctgccc gcgaagaata ttcaggagct gatccaggca gccaaggccg caccggacaa
     5341 gctgacgttt ggctcggccg gcaacggcaa taccgggcac ctggcggccg aatatttcag
     5401 cagcatcgcc ggcatcaaga tgacccatgt gccctacaag ggcagcgccg gcgtggttaa
     5461 tgccatgctg gccggcgata ttgacctgac cttcgacagc atctccacct ccatgccgca
     5521 catacgcagc gggcgcatgc atgcgctggc ggtcaccagc gcgcagcgct cggcactggc
     5581 gccagatgtg gcgacggtgc aggagcaggg cgtggccggt tttgacgtga cgggctggta
     5641 tgcgctgatc gcccccgccg gcaccccgtc ggaggtcacc cagcgcctga gcagcgagat
     5701 tgccagcgcg ctgcgccagc cgcagctgca aacgcagctg gctgccggcg gctacgtgcc
     5761 cgtgggctcc acgcccgagg cgctgcaggc acatatcgag cgcgaaatca cgcgctgggc
     5821 cgccgtggtc aagtccactg gggccaaggt ggattgaccg cgcgctgtga ggtgacgtcg
     5881 gtcaccgggc cttgcgtggt gccgggtcac gaactggttg aataattaag atacatagat
     5941 aaaatatttc atcagaaata tctaacaacc agggttcgcc atgcaacgcg ccgccaccac
     6001 accccaaccc gaccccgccg ccgtgctggc caaggccacg gcccgggcca gtgagctgct
     6061 ggggctgacg ggcgtcgccc tggcccgcac cattggcctg agcgagccca cggtctcgcg
     6121 catcctgcgc ggcgagaaga ccatagcccc cacctccaag gagggcgagc tggccctgct
     6181 gctggtgcgc gtgtaccgtt cgctggatgc gctggtgggc accgacgacg ccaggcgccg
     6241 agcctggatg gacacctaca accaggccct gggcgggcgg ccgctgcaac tggtgcaacg
     6301 ggccgacggt ctggtggcca cgctgaacta cctggacggc atgcgggcgc ccgcgtgatg
     6361 actttggcgg ccgactggga gcgcttctgg ctggagcctg gcataggtgc ccaatccatg
     6421 ctggcctggc gcggggtaga ggcgcagcac gtggtctcca ccatgcgtct ggtggacagc
     6481 gcggccgagc aggatttgct ggagcaattg cttgaagcca gcaagcccgc gctgcctgca
     6541 acacgccagc cccggcacta cctgctgctg acgccgttcc gctaccgccc tcatcacccc
     6601 agccgcttcc ggccggcgca cgcgcgcggg cagtggtatg gggccgaagc actgtacgcg
     6661 gcctgtgccg aggtggcgta ctggcgccgg cggttcattc tggacagcac cggtttgcag
     6721 gatcagacgc tactgaccga gcacagtttt tttcaagcgg ccatcgatgg ccaggccatg
     6781 gacctgatgg cgccgccctg gaaccaggcg cgcgcgctgt ggacgcaggg cgacgactac
     6841 gccgccacgc aggccgtggc ggcaggcgcg cagcaacgcg gcgtgcagtg gctggcctat
     6901 gagtcggcac gcgcaagcgg ccagcgctgc gccgtggtgt tcgacccgga ctgcctggcc
     6961 gagcctgcgg gcgggctgga tcacaccctg cagacctggc actgcaaggc cacgcgcgcc
     7021 agcgtgatgt tcacgcgcgg gcgcgagcgc catgaatggc atttttgaat tttgaaccta
     7081 caggaaccag aaccatgagc agcaacgaca tccccctgag cccctttgcc ggcgaggccc
     7141 ccggcctgct gtttggcctg cccatgccca tgtcgcgtgc catggcgctg cgcggcgagc
     7201 gcataggcaa cgaccgggcc caggtgcgca tgggcttcca ggccgagcag tccaacagcc
     7261 gcggtgacgt gcatggcggc gccattgcct cgctgctcga ttgcacgctg gccggcgccg
     7321 cgcgcgccca tgaccctgcc gcctatggtg tggtcaccat agacctgacg ctgcactttg
     7381 tctccgccgc cagcggcgac ctgattgcca ccgcgcattg cgagcggcgc gggcgctcca
     7441 tcagctttgt gcgcggcgag gtgcgcgccg aggatggcac cctggtggcc ctggccacgg
     7501 gcaccttcaa gctggtggcg cggcagccgg cgcggggctg aaaccagcgg cgcccgtgcc
     7561 ccccatgccg gcaaggtgcc ggcgctcccg ggttcagttg ggctgcacgc cggcggcctg
     7621 cagcaggcgg ccccagatcg gcccttcctg cgcccacagc ctgccaaagc tctcgggcgt
     7681 gtcgtcgtcc accggcacga agcccgcggc gatgatgcgc gccatgcctt cgcggctggc
     7741 cacggcctgg ttggcagcct gggcccattg gcgcgcgaca tcggcgccca tgcccttggg
     7801 cccggcgatg gccagccagc cgaccagggc cagggcctcc tgcttgaggc cggcctcggc
     7861 aaacgtgggc acatcgggca gcagcggtac gcggcgcggg ccggtcacgg ccagggcgcg
     7921 caggcggccg ctgtcgatat gcgccttgat ggacagcagg ctgcccatgc cgatcttgat
     7981 ctgcccaccc agcaggtcct ggatcatggg cgcttcgccc ttgtaggcca catgcgccat
     8041 gtcggcgccg gcaacgtcgc tgagcgtggt gcaggccagc tggccgtgcg agcctatgcc
     8101 gtaggagccg taggacagct tgcccttgtt ggcgcggagc caggccatga actcctgcag
     8161 gttctgcgcc ggcacatcgt tggtcaccac cagcgcaatc ggcgccaggc atacgcgcac
     8221 cagcggcgtg aggtcggcca gcgggtcgta gggcggcttc ttgtagagat atttgttggt
     8281 cagcatggac gaggtcgtgc ccagcagcac cgtcatgcca tcaggcggcg acttgaccac
     8341 catctcaccg gcgatcaggc cggcgccgcc gggcttgttg tccaccacca ccggctggcc
     8401 aaagcgtttg cccatgtgct cgcccagcac gcgcgtgatg acgtcggtgg cgccgccggc
     8461 ggcaaagggc acgaccactt tgaccgggcg cgtggcaaag ccggtttgcg cccgcgcggc
     8521 gagcgggccg gcggctgcgg cgccggcggc aagcgccagc agctggcggc ggttgcatgt
     8581 ggggggctgc atcggcttgt tcatggtttg tctcctcgtg ttgtcgtgtc gtgaccgttc
     8641 aggtggggcg cgctgtggtc tgtaggagcg gctttagccg cgaatgacgc cgttcgcggc
     8701 taaagccgct ctacaagaac cgggcggtca atccatggag atttttgcac cctcggccac
     8761 ggccttccag tgggcggcgt ctttttctac ctgggtcttg aagtcgtcgg gcagcgagcc
     8821 cacggcgatc aggcccatgt cggtcaggcg cttgttcagt tcgggcgact tgacgatcgc
     8881 agccacctcg gcgcccagct tgtccacgat ggccttgggc gtgctggccg gcaggaacac
     8941 gccaaaccag ccattggcct ccagcccggg gtagccggat tcggccatgg tgggcacgtt
     9001 cggcagcgag gggaagcgct gtgagccggt cacggccagg atgttcagct tgtccgactt
     9061 gatgtgcggg taggcggccg tggcgtccac gaaggccagc gtgagctgcc cgcccagcag
     9121 ggccgccatc tcggggcccg agccctggta ggggatgtgg gtcaggtcta tgccggcctg
     9181 ctgcttgaag cgctcgccgt tcatgtgcga cgaggtggcg ttgccgtagg tgccgtagtt
     9241 catggtgccg ggcgcggcct tggccttgtc cacgaactgc gccagcgtgg tcacgcccga
     9301 gctgcgcggc accatcagca ggtcggccga cttggcgatc tgtgacaccg gcgccaggtc
     9361 ggccagcttg tacggcacct tcttgtacag cgccggtatc tgcaccacgg cggtgatgcc
     9421 catgagcacg gtgtagccgt cgccgggcgc cttggccacg atgtcgttgc ccaggatgcc
     9481 gctggcgccg ggcttgttct ccaccaccac cggctggccc cagcgctggg tcagctgcgt
     9541 gcctatcagg cgggcgatgg tgtcggtgcc gccgccggcc gggtagggca cgatgaagcg
     9601 caggggcttc gctggatagt cgccctgtgc ctgggcgctg ctggtgccga gggtggcgca
     9661 ggcggccagg gcgatcaggg ccagggtggt gcgtcggttg cgtgtcatgt ctctctcctt
     9721 gtacttgggg gctcacaaag tgcgggcgat gatctctttc atgatctcgt cgctgccgcc
     9781 gtagatgcgg cctatgcgcg cgtcggtcca ggcgcggccc acctggtatt cggtcatgta
     9841 gccgtagccg ccgtgcagct gcaaaaattc gtccagcagc cggttctgca gcaccgtggc
     9901 gttgagcttg accatggcgg cgcgttcggg cgtcagttcg cggcgcaggt gcagggcgat
     9961 gcagtcgtcc acaaagacgc gcagcatggt ggcctgggcc ttggcctcgg ccagcttgaa
    10021 gcgggtgttc tggaactcga acaccgtctg gccaaaggcc ttgcgttcgc gcgtgtagtc
    10081 cacggttttt tgcaaaaagg tctcgaccga ggcggcggcg cgcacggcga ccaccaggcg
    10141 ctcctgggcc agctcctgca tcaggtacct gaagcccagg ttctcctcgc ccagcaggtt
    10201 gcccacgggc acgcgcacgt tgtcgaagaa cagctcggag gtgtcctgcg ccatcaggcc
    10261 tattttttcc agcttgcggc ccttgctgaa gcccggcgtg ccgtcctcga ccacgatcag
    10321 cgacacgccc ttggccccca gctcgggcgc ggtcttggcg acgacgatga cgatctcgct
    10381 gttgatgccg ttggtgatga aggtcttgct gccgttcagg acgtagtggt cgccatcgcg
    10441 cacggccgtg gtgcgcacgg acttgaggtc gctgcccgcg ccgggctcgg tcatggcgat
    10501 cgcgccgatc agctcgcccg ccgccatgcg cggcagccag cggtccttct gctccttgtt
    10561 gccgtaggcg aagatgtacg gcgcgacgat gtccgagtgc agcggaaacc cgaggccgct
    10621 ggcgccggcg cgggcgattt cttcgagcag cacggcggca tggccaaagt cgccgccgcc
    10681 gccgtagggc tcgggcagca tggggttcaa gaggccctcg cgcccggcct tgcgccagac
    10741 ctccttgggc acgatgccgt cgcgctccca ctgggcatgg tgcggcacga cttcgcgctc
    10801 gaagaagcgc cgcgcctgct cgcggaactg ctcgtggtct tcgcggtaga tgctgcgggt
    10861 ggtcaggtct tgcatgatgg aactcgctga tgggttgggt gaaattgcct ggctatgcac
    10921 atgtcgctga ggcccctcac ccctcccggg ggcacagcga gacgctgtgc tcttcaggct
    10981 gtccagttcc gcgcatgcgg aactggagcc gcagccttca gccccaaagg ggggagggcg
    11041 accggttgcg ccggtatgac tccctctccc tctgggagag ggtgggggtg agggcttacg
    11101 gccggtctga gacatatcgc taatcaagtg aaatttggtt gaagcgcttg atggataagc
    11161 gcagacagct attgttttaa gagtctcgca gggcgaatcc tgcctgcggc gcatcgcggt
    11221 actgctcctt gagcttgcgc cgcagcagct tgccggtctc caggcgcggc aggctctcga
    11281 caaacaccat gcgctgcggc agcttcaggc gcgccagcac cacggcggcc tggccggcga
    11341 tggcgcgcgc cgtggccggc gagggctctg cccctgggcg cagcaccacg gcggccagcg
    11401 gcacctcgcc aaagtcgggg tgcggcacgc cgaccacggc cagctcctgc acatcggggt
    11461 ggcgcgccag ggcgttttct atctcctgtg ggtacaggtt cacgccgccc gagaggatca
    11521 ggtcggcgcg tcggtcgctc aagaacagat agccatcggc atccacatgg ccgatgtcgc
    11581 cataggtgaa ccagccttgc tcattcagcg cctggcgcgt cttctccggg tcgttcaggt
    11641 agctgaaagt cccgccgcca gagaagaaca cctggccgat ctcgcccgcg ggcagctcgc
    11701 ggccctggtc gtccacgatg tgcagctggc ccgtggtcgc gcggcccacc gagccggggc
    11761 gcaaccgcca ttcggcggaa ttgatcatgg tcgtgccgca gccctcggag ccggcgtagt
    11821 actccatcag gatgtcgccc caccagtcca gcatggcgcg cttgacatcc accgggcagg
    11881 gcgcggccgc gtgtatggcc acgcggtggc tggataggtc atggcgccgg cgcagctctt
    11941 cgggcagccg gagcatgcgg gtgaacatgg tcggcaccca ctggctgtgc gtgacgcggt
    12001 ggcgctctat cagctccagc gcgctttgcg cgtcgaagcg ttccatgatc accgcactgc
    12061 cgcccagctc cagcacgcgc agcgtgtagc gcagcggcgc ggcgtggtac agcggcgcgg
    12121 gcgagaggta gaccgtgcgc tcgtccactt gcatcaggcg cgccgtgccc agggcctctg
    12181 ggtcgcactg gccgcgcagg ctggcgggcc acaggggttt gagcaccccc ttgggccggc
    12241 ccgtggtgcc ggacgaatac agcaggtcgc gccccagggg gcggtcgctc aggtcgatgg
    12301 ccgggcctgg cagcgccgcc agcgcggcgg gcagcgatgc ggcctgtggc tgggcttcgt
    12361 ccaccgtcca gcagggcagg gccagggtgc ggggcaggtc ggccagctgc ggcagggtct
    12421 ggctggatgc cacgatcagg cgcgcgtcgc agtcctgcac gatgtaggcc acctcgtcgg
    12481 ccgtcagatg cgtgctgagc acggcggcgt acaccccggc ctcgcgcgcg gccagcacca
    12541 gggccaggat ctcggcacgg ttttccagca gcacggcaaa gcgctcgccc ggctgcaggc
    12601 cctgggccgc cagccactgc gccatttgca gcgcctgctg cgccagcgcg ccggcggtgc
    12661 agctgcggcc ctgttcggcc aaaactacca ccacccggtc gggggccgcc ttggcgcggc
    12721 caaagaagtc ctgcatgcct gtcacccttg gttctgcagg cgcatggccg ggatggccct
    12781 gcgccaggaa ttgcgaaata cgtgtctcac aaatcacatc ttgagccgga ttctttcagc
    12841 cattacatcg acaaagcatg gggaaaacac cgcgttaagc agatactaac ggcgtgatta
    12901 ttggttttaa tggatcattt gtttcattaa ccgcatggag tgcacatgaa aaatcccgtc
    12961 atcgtcgatg ccatccgcag tcccatggcc cgggccaagc ccgatggcgc gctggcccag
    13021 ctgcaccccg tggatctgct gtcgcaggtg ctgcagcagc tggtggagcg caacaagatc
    13081 gaccccggcc aggtcgatga cgtgatctgc ggctgcgtca gccaggccgg cgagcaggcc
    13141 ggcacgcccg ggcgcctggc ctggctggcc gccggcctgc ccacgcatgt gccgtcgacc
    13201 accatcgatc gcaaatgcgg ctccagccag caggcggtgc acttcgccgc ccaggccatc
    13261 atggccgggg tgcaggacat ggtcattgcc tgcggcgtcg agtcgatgag ccgcgtgccc
    13321 atgggcagct cgcgcatggg ccagaacacc accggcgcgc gcttcgatgc gcgctacgcg
    13381 cccggcctgg tgggccaggg cgtgtcggcc gacctggtgg ccgccaagtg ggggctgagt
    13441 cgcaccgagc tcgacgccta cgccgcccgc tcgcaccagc gcgccgctgc cgcgcagtcg
    13501 gcgggccggt tcgagcgcga gatcgtagcc atcgatacgc ccgccggccg cgtgctggca
    13561 gacgagacca tccgagccgg caccacggtg gaaaagctcg ccggcctgaa ggccgtgttc
    13621 gagagcgagg agctctccaa gcgctttccg caaatccagt ggagcaccac cgccggcaat
    13681 gcctcgcaga tgaccgacgg cgccagcgcc atgctgatca tgagcgagga gcgggcgctg
    13741 cagctgggcc tcaagccgcg cgcgcgcttc gtggccttcg acgtggtggg cgacgacccg
    13801 ctctacatgc tgaccgcccc catccccgcc acccagcgcg tgctggccaa ggcgaagatg
    13861 aagctggaag acatacacca ctacgagatc aatgaggcct tcgccagcgt gcccatggct
    13921 tggcagaagg agttgcaagc cgacggcgag cgcttgaacc cgcgcggcgg cgccatcgcc
    13981 ctgggccacc cgctgggcgc ctcgggcatc cgcctcatga ccaccatgct gcacgcgctg
    14041 gaagacagcg gccagcgcta cggcctgcag tccatgtgcg aggccggcgg catggccaac
    14101 gccaccatca tcgaacgctt gtaatttccc ccgtcttctc cctcgccccc ttgggggaga
    14161 gggcaggggt gagggggtag ttcaggcatg acggctgatg ttgccatcca ccctcacccc
    14221 tgccctctcc cgccagcggg agagagagta accgccaaaa aatggagtca acatgaaact
    14281 gcaaccagga atgaccgccc tcgtcaccgg cgccgtctcc ggcctgggcg aggcatctgc
    14341 catcgccctg ctggagcgcg gcctgaatgt cgtcgccgtc gatctgaacg acgagcgtgg
    14401 cgccgccatg gaggcgaaat accccggccg cctgcgctac gtgaaggccg acgtctccga
    14461 cgaggcgcag atgcaggccg cggtcgccgc agccgaggcc tttggcaact tccgcgccct
    14521 ggtgcattgc gccggcatag gcgggccgct gcgcctgatc gagaaggacg gcagcgccgg
    14581 atccttggag aagtacacca acatcatccg tgtcaaccag atcggcacct tcaacaccct
    14641 gcgcctggcg gccgtcgcca tggcgaaaaa tgaaccggtg gacggcgagc gcggcgcctg
    14701 cgtgctcacc gcgtcgatag cgggctatga ggggcagatc ggccagatcc cctacgcctc
    14761 gtccaaggcc ggcgtgatcg gcatgaccat cgtcgccgcg cgcgacctgg cgaccaagct
    14821 gatccgcgtc tgcaccatcg ccccgggcct gttcgacacc cccatcctgg ccaagctgcc
    14881 cgagaacgtg cgccagtcgc tgggcgcctc cgtgccccac ccggcgcgcc tgggccaccc
    14941 cagcgagtac gcgctgactg cgctgcacat cctggagaac cccatgctca acggcgagac
    15001 catccgcctg gacggcgcca tccg
//
LOCUS       DAOMTQ010000067        14991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_633_length_14991_cov_29.140064, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000067 DAOMTQ010000000
VERSION     DAOMTQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14991
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_633_length_14991_cov_29.140064"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="PK518_08270"
     CDS             complement(<1..1754)
                     /locus_tag="PK518_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HRM48949.1"
                     /translation="MCGISGIFDTQGTRAYSRELIVRINDAQAHRGPDEHDVHLEPGL
                     ALGHRRLSVIDLAAGQQPLFNQAGTVGIVFNGEIYNYRDLTDELKALGYHFRTRSDTE
                     VIVHAWSAWGEACVHRLRGMFAFALWDRDRQTLFLARDRMGVKPMHYAWLPDGSFVFG
                     SELKVLTAHPGFARDIDPLAVEEYFTFGYVQDPRCIYRHAHKLPAAHTLTLKRGEKNR
                     ASACPYWDVSFTNNSIITLQDAQAELRERITESVRLRMIADVPLGAFLSGGVDSSAVV
                     ATMAGLSSAPVQTCSIGFDDPRFDESSYAKQVAERYRTNHRMEIVSSDDFDLIDTLAW
                     LYDEPYADSSAIPTYRVCQMARKHVTVVLSGDGGDESLGGYRRYRMHLGEEAVRGRLP
                     LALRRGMFGPLGRWYPKADWAPRSLRAKTTFQALAMDSVQAYCHSMSMLRGDQRQALF
                     SPGLQRSLGGYHALEVFRQHAARAQTDDPLALIQYLDYKTWLIGDINTKVDRASMAHS
                     LEVREPLMDHLLVEWLATLPSDFKIRGGQGKAIFKQAYEPLLPHDLLYRPKMGFSVPL
                     AAWLRGPLKDRVRSALQG"
     gene            complement(1757..3328)
                     /gene="xrtA"
                     /locus_tag="PK518_08275"
     CDS             complement(1757..3328)
                     /gene="xrtA"
                     /locus_tag="PK518_08275"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HRM48950.1"
                     /translation="MTASKPLIPAYWHRPLAAFVLLLVVILAAYWHSAWAMAMTWTRS
                     ETYAHGFVVPLITLWLVWRQRAVLAGMVPRPGSLAWLLMAGAAALWLAGDLVAVNAAT
                     QLALVSLIVLAVPAVLGWQVTRALAFPLAFLFFAVPIGDFLLPRMMEWTADFTVLALR
                     ASGIPVYREGLQFIIPSGAWSVVEACSGIRYLIASVTAGCLFAYLSYQSMLKRAVFIG
                     VAIVVPLVANWVRAYLIVMLGHLSGNTIATGVDHLIYGWLFFGLVIGVMFLIGARWVD
                     PPAPAPKAMALAQADAPRQATWLGLALALVVVLAPYGVHAMIGLTAQTAPVHLVQPQV
                     AAPWTAAAQPPNTWVPMFRHASADLNMGLLDAQGRPAGLHISYYRQQDYERKLISSEN
                     VLVFSNDPTWSRVTGGSTVAQLQGKPLQVESAVIRNNTRGMAAGGERFLAWRFYWVHG
                     GFTASDYVGKLQGALGRITGWGDDGANIVIYAPLSAEMDVKGAEADAAARLQAYLDSQ
                     GDALAAALKQTRGRD"
     gene            complement(3339..4595)
                     /locus_tag="PK518_08280"
     CDS             complement(3339..4595)
                     /locus_tag="PK518_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HRM48951.1"
                     /translation="MAAVSNILFLVHRLPYPPNKGDKVRSYHLLRHLQKNHRVFLGTF
                     IDDPDDVQHLPVLQAMCKDLHVEHIEPRSAKIKSLSGLLTGEPLTLTYYRNAGMQRWV
                     QQTVAEHKLQACVVFSSAMAQYAQPLLPDLPMLVDFVDVDSAKWTQYAPAHRWPLSMV
                     YRREGRRLLAYERAVAAQAHCSYFVTPNETALFVSQAPEAKGRVQSMGNGVDCDFFKP
                     DAQLANPFDQGEQALVFTGAMDYWPNIDGVSWFVADMLPRLLARHPGLRFYIVGRSPS
                     PQVKALAGANVVVTGTVPDVRPYLQHASAVVAPLRVARGIQNKILEAMAMQQPVVTVS
                     SCADAIGATAAQGLLRADTAEEFVAALLELLQSPTDRVALGECARHYVEQECSWQAHL
                     KKIDHDLASTLAQPVASASSTATATA"
     gene            complement(4609..5664)
                     /locus_tag="PK518_08285"
     CDS             complement(4609..5664)
                     /locus_tag="PK518_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HRM48952.1"
                     /translation="MSDVVTSLSIQRLDRRNSIDVARWDAFVMACPQATFFHRSGWQV
                     LIEDIFGHDSYFLYAESGGVIRGVLPLGHVKSRLFGKALTSLPFSVYGGVAAVDEETA
                     TALELRAQQLARELGVDHLELRNVQRRHADWPLQDLYVTFRKPIEATEEKNLLAIPRK
                     QRAMVRKGIKNGLTSQIDPTADRFFALYADNVHRHGTPAMPKAYFNQLLRVFGPDCEV
                     LTVCAPDGKPVSSVLSFYFRDEVLPYYAGDAEAARELAANDFKYWELMRLACARGLKV
                     FDYGRSKLNTGSYAFKKNWGFEPTPLHYEYQLYKTDSVPQNNPANAKYRLLIATWRRM
                     PIQLANWLGPKIVRNLG"
     gene            complement(5657..6523)
                     /locus_tag="PK518_08290"
     CDS             complement(5657..6523)
                     /locus_tag="PK518_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRM48953.1"
                     /translation="MSITPPITNALTIDVEDYFQVSAFAPYIQRSNWDSRECRVERNV
                     DQILKLLAQHNVQATFFTLGWIAQRYPKLVRAIVDGGHELASHGYGHERVSDLSERQF
                     FDDIHSAKSLLEDIGGVEVQGYRAPSFSIGSQNLWALDTLQRAGYRYSSSIYPIQHDH
                     YGMPDAPRFAYEARDGLMEVPPTTMRMFNRNLPSSGGGYFRLLPYALSRWMLRQVNSK
                     DASPAVFYFHPWEIDKNQPRVEGVGLKTRFRHYVNIGRMEQRLGCLLRDFRWGRMDHI
                     FLPQSVQKASLV"
     gene            complement(6532..7653)
                     /locus_tag="PK518_08295"
     CDS             complement(6532..7653)
                     /locus_tag="PK518_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HRM48954.1"
                     /translation="MYEAFYGLTSKPFQLNPDPGFYFSSKQHQRARAYLEYGVMCCEG
                     FIVITGEVGAGKTTIVRGLLDSLDSDSVVAANLVSTQLGADDTLRLVGAAFGVPVNGM
                     SKGDLLMSLEAFFVAQTLQGKRCLLVVDEAQNLQPQAVEELRMLSNFQNGQDALLQTF
                     LVGQPEFREILQSPGMLQLRQRVIATCHLGPLDAEETREYILHRLKCAGAAEKPTFDD
                     EVFDEIYRFAGGVPRRINTLMDRVLLQGFLADKSHIDVELVIGIIHEVQSEMQVPPKT
                     LPPWNTPLRATGGSSAYDTSVDAEYGLDSIELDADVADGISDQLMQMTIEQLRAHLLR
                     IERSVLRQERASLEILMTLQKLVAASRRPRPDSNVSSNG"
     gene            complement(7657..9279)
                     /locus_tag="PK518_08300"
     CDS             complement(7657..9279)
                     /locus_tag="PK518_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HRM48955.1"
                     /translation="MYLPHVGLVRIPAIGWAAWVLASAPGLVAAQVPDLGSDPQSRTA
                     STQSPAGSGAAARSGSDQPEPRASGQWFEPRISVQHTVTNNSRLIHNGSGDQITEVMP
                     GFRLARDTARLKGFVDYTLRAVNYARDTSPDKIWHNLYARGTVEAVEDHVFVDVDGRA
                     ALQPISAFGPAGGANTANTNLTQATSFSVSPYIKGDLTDGLSYEARYRMTDTRYDTAA
                     RSNVMVHDWLLQLEKRPLGQLMSWTVEAAQQNAEYTNGRNIDTTALRGRLGYMPRSGL
                     RLVAIGGAESTNQLSPIRETSSILGFGVDWRPSERTSVSFERVKRYFGESHNAVAQYR
                     TGRTVWRYTDKKSVVNGLGDQFGSPSPLYDLLDGFYMRTEPNAIRRMQLVQAEIERMG
                     LPADMQVFQDFLTSSSKLQRLQNLSFAILGQRSTLTVAVMRSDSRLLQGAFSLGDDFS
                     NDSKIRQRGWNISASHRLTPSSGVTLSFGEMRNSGVDTGLETRTRPFIFSWTSLVARR
                     TSVGLQLRRVLSDGNVSQYGESAIMGFITHRF"
     gene            complement(9296..10264)
                     /locus_tag="PK518_08305"
     CDS             complement(9296..10264)
                     /locus_tag="PK518_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HRM48956.1"
                     /translation="MTSSIEKAAQRLEQLRKAGAVLPGDKPRVQNPGVPDESQPLNHV
                     PQFAAPAADGDHAAPELRAVSKTVQLDLTALSASGFVTPGSPRSVTTDQYRVIKRPLL
                     ENAMGKGASLVTNGNLIMVTSALPGEGKSFTAINLAMSLAMELDHTVMLVDADVTRPS
                     VMSKLGLPPAAGLLDMLRGGQTKLADVLLRTNVEGLTLLPAGLPHDRATELLASDAMT
                     DLLDEMSKRYSDRIIIFDSPPLLLTTEARVLANHMGQVVVVVRAERTLQSEVEHALST
                     IEACPIKLLVLNQVRSQGLGAHGYGYGYGHGGYGRATEERMPAAAA"
     gene            complement(10274..11836)
                     /locus_tag="PK518_08310"
     CDS             complement(10274..11836)
                     /locus_tag="PK518_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRM48957.1"
                     /translation="MDELLSQITTTARGMWIYRRLAMLTIWVVGAIGAGVVLTMPDYY
                     QASARVFVDTQSILRPLMTGIAVQPNIEQQVSMLSRTLINRPTVERLVRMADLDLGST
                     TKASTDAVVDAVTQSIKIQSTGRDNLYTLSYRDQSPEKAQLVVKSLLTIFVESSLGAT
                     RQDSDSARRFLDDQIKTYETKLTEAEGRLKAFKLRNIEMQSEEGLDSAGRASQIENTL
                     SQARLELREAESARAAAAQQLEEMRAQTAKSMAGPAPDVQTPELDARLLAQKQNLDAL
                     LQRYTDGHPDVIRTRTLIADLEGQKRKEIDVLRRKLQQTGEVPIAQVNPAAVELSRIY
                     SAAQVQVASLRARVVEYEARVQRVHQQLKVAPQLEAELAQLNRDYQIHQKNYADLVAR
                     RESALMSGKLENTSNVAEFRVIDPPRVAPKPVAPNRLLLMPGAMLAAVAAGLGIAFVM
                     SQIRPVFFDGATLRQVTQLPLLGVVGLIPSEEQRRRESRSLKRFIMATVAFLLLYAGG
                     MAALSIRTGALG"
     gene            complement(11886..12521)
                     /locus_tag="PK518_08315"
     CDS             complement(11886..12521)
                     /locus_tag="PK518_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HRM48958.1"
                     /translation="MRGFFSRLVGSVGRAAAVVAVAVTAVGCAGTGNYPAAPVKASSP
                     DYNYVVGPGDTLNIIVWRNPDLSLSVPVRPDGQVSLPLIDGLQAQGKNSAQIARDIEK
                     ELAKYVRDPVVTVIVTNFVGPYSEQIRVVGEAAKPQFLPYKQKMTVLDVMIAVGGLTD
                     FADGNSATLVRAAEDNKRYKVRLHDLIKRGDIDANVEVLPGDVLIIPQGWF"
     gene            complement(12670..14112)
                     /locus_tag="PK518_08320"
     CDS             complement(12670..14112)
                     /locus_tag="PK518_08320"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRM48959.1"
                     /translation="MLIQPVVLSGGSGTRLWPLSREKYPKQLLSFVGQDSLLQATLRR
                     LEGIDGATCAPPLVVCNDEYRFVVAEQLRLMGLQGRIVLEPVGRNTAPALTLAALAAR
                     AAGDDPVLLVMPADHVVSQVAAFQECVRHAARLAQNGAVVTFGITPDRPETGYGYIQA
                     GAALDAGGACAIARFVEKPDRATAEGYLQQGNYSWNSGVFVLRASVWLNALRLCRPDI
                     LEACEAAWNKSTSDLSFVRVDAEAFAACPSDSIDYAVMERLAGAQAVEGLPQGVVLPL
                     SAGWSDVGAWDALWQILPKDEAGNVSQGRVMLQDCNDTLAYSSGRLLACVGVQSLVVV
                     ETPDAVLVVDQRHTQDVKKIVDRLKREGCAEGMLHRKVYRPWGWYDGVDAGERFQVKR
                     IMVKPGGKLSLQMHHHRAEHWVVVRGTARVTKGDEAFLLTENQSTYIPLGVTHRLENP
                     GHVDLEMIEVQSGSYLGEDDIVRLGDVYGR"
     assembly_gap    14610..14709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14741..>14991)
                     /locus_tag="PK518_08325"
     CDS             complement(14741..>14991)
                     /locus_tag="PK518_08325"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207696.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HRM48960.1"
                     /translation="EKLKSNMQEVRARGGVLYVLADANTNIESSEGVHVIRMPEHYGS
                     LSPLLHVVPLQLLAYHTACARGTDVDKPRNLAKSVTVE"
BASE COUNT         3077 a         4676 c         4260 g         2878 t
ORIGIN      
        1 tcgccctgca atgccgaacg cacgcggtct ttcaaggggc cgcgcagcca tgcggccagc
       61 ggaaccgaaa aacccatctt gggccggtac agcaggtcgt gcggcagcag cggttcatag
      121 gcctgcttga agatggcctt gccctggccg ccacggatct tgaagtcgct gggcagcgtg
      181 gccagccatt cgaccagcag gtgatccatg agcggctcgc gcacctccag cgagtgggcc
      241 atgctggcgc gatcgacctt ggtgttgatg tcgccaatca gccaggtctt gtagtccagg
      301 tactggatga gcgccagcgg gtcgtcggtc tgcgcccgtg ccgcgtgctg gcggaatacc
      361 tccagcgcgt gatagccccc caggctgcgc tgcaagccag gcgaaaacag ggcctggcgc
      421 tggtcaccgc gcagcatgga catgctgtgg cagtaggcct gcacgctgtc cattgccagc
      481 gcctggaagg tggtcttggc gcgcagcgag cgcggcgccc agtcggcctt ggggtaccag
      541 cgccccagcg ggccgaacat gccgcggcgc agcgccagag gcaggcggcc gcgcacggcc
      601 tcttcgccca ggtgcatgcg gtagcgccgg tagccgccca ggctttcatc gccaccgtcg
      661 cccgacagga ccacggtgac atgcttgcgc gccatctggc agacgcggta ggtggggatg
      721 gcggagctgt cggcgtacgg ttcgtcgtag agccaggcca gggtgtcgat caggtcgaaa
      781 tcatcgctgc tgacgatctc catccggtgg ttggtgcgat agcgctcggc cacctgtttg
      841 gcgtagctcg attcatcaaa gcgcgggtcg tcaaaaccga tggagcaggt ttgcaccggc
      901 gcgctggaca ggccggccat ggtggccacg acagcgctgg aatccacccc gccagacaga
      961 aaggcgccca gcggcacatc ggcaatcatg cgcaggcgca cggattcagt gatgcgctcg
     1021 cgcagctcgg cctgggcatc ctgcagcgtt atgatgctat tattagtgaa gctaacatcc
     1081 caatatggac atgcgctagc cctgtttttt tcaccacgtt tcagcgtcag cgtatgggcc
     1141 gctggaagct tgtgcgcatg gcggtagatg cagcgcgggt cttgcacata gccgaaggtg
     1201 aagtactcct ccaccgccag cgggtcgatg tcgcgcgcaa aaccggggtg tgccgtgagt
     1261 accttgagct ctgagccaaa gacaaaactg ccatcaggca accaggcgta atgcatgggc
     1321 ttgacgccca tgcggtcgcg cgcaagaaac agggtctggc ggtcgcgatc ccacagcgcg
     1381 aaggcaaaca tgccgcgcag ccggtgcacg caggcctcgc cccaggccga ccaggcatgg
     1441 acgatgacct cggtgtcgct gcgcgtcctg aagtggtagc ccagcgcctt gagctcgtcc
     1501 gtcaggtcgc ggtagttgta gatctcgccg ttgaagacga tgcccaccgt gccggcctga
     1561 ttgaacaagg gctgctggcc ggcggccaga tcgatgacgg acagccgcct gtggcccagt
     1621 gccaggccag gttcgaggtg cacgtcgtgc tcgtctgggc cgcggtgggc ctgtgcgtcg
     1681 ttgatgcgca cgatgagttc gcgcgagtag gcgcgcgtgc cctgcgtgtc aaagatcccg
     1741 gatatgccgc acatgctcag tcgcgccctc ttgtttgctt cagggctgcc gccagggcat
     1801 caccctggct atccaggtag gcctgcaggc gcgccgccgc gtcggcctcg gcccccttga
     1861 cgtccatctc tgctgacagc ggagcgtaga tcacgatgtt ggcgccatcg tcaccccagc
     1921 cggtgatgcg ccccagggca ccctggagct tgcccacgta gtcactggct gtgaacccgc
     1981 cgtgaaccca gtagaagcgc caggccagaa aacgctcgcc accggcggcc atgccacgcg
     2041 tgttgttgcg gatcaccgcg ctttcgacct gcaatggctt gccttgcagt tgcgccaccg
     2101 ttgacccgcc ggtcacgcgc gaccaagtcg gatcattgct gaagaccaat acgttttcgg
     2161 agctgatgag cttgcgctca tagtcctgct ggcggtagta gctgatatgc aggcctgctg
     2221 gcctgccctg cgcgtccagc agccccatgt tcagatcagc ggatgcatgg cgaaacatgg
     2281 gtacccaggt gttggggggc tgcgctgcgg cagtccaggg cgcggcaacc tggggttgca
     2341 ccaggtggac aggtgctgtc tgggccgtca acccgatcat ggcgtgtacg ccgtagggcg
     2401 ccagcaccac cacaagggcc agcgccaggc caagccatgt cgcttgcctg ggcgcatctg
     2461 cttgtgccag cgccatggcc tttggcgccg gcgccggcgg atccacccag cgcgcaccga
     2521 tgagaaacat cacgccaatg accaggccaa agaacaacca gccatagatg aggtgatcca
     2581 cgccggtggc aatggtgttg cccgagaggt gccccagcat gacgatgagg taggcgcgca
     2641 cccagttggc caccagcgga acgacaatgg cgacaccgat gaacaccgcg cgcttgagca
     2701 tgctctggta gctgagatag gcgaacaggc agccggccgt gaccgacgcg atcaggtagc
     2761 gaatgccgct gcaggcctcg accaccgacc acgcccccga cgggatgatg aactgcaggc
     2821 cttcgcgata aaccggaatg ccactggcac gcagggcgag caccgtgaaa tcggcagtcc
     2881 actccatcat gcgtggcagc aaaaagtcgc caatgggtac ggcaaagaac agaaacgcca
     2941 acggaaaggc cagcgcccgg gtgacctgcc agcccagtac cgcgggcacc gccagtacga
     3001 tcaaggacac cagcgcgagc tgggtggctg cattcacggc caccaggtca cctgccagcc
     3061 agagcgccgc ggcccctgcc atgagcagcc acgccagact gcccgggcgc ggcaccatgc
     3121 cagccagcac ggcacgttgg cgccacacca gccataaggt gatgaggggt acgacaaacc
     3181 catgggcata ggtctcggag cgcgtccagg tcatggccat ggcccaggcc gaatgccaat
     3241 aggcggccag aatcaccacc agcagcagca cgaatgcggc cagggggcgg tgccagtagg
     3301 cgggaatcaa tggcttgctg gccgtcatat cgagcctgtc atgcggtggc ggttgcagtg
     3361 gagcttgcgc tggcgactgg ctgcgcaagc gtgctggcca ggtcgtgatc gatttttttc
     3421 aggtgggctt gccagctgca ttcttgctcg acgtaatgcc gggcacactc gcccagcgca
     3481 accctgtcgg tgggcgactg caacagttcc agcagcgcag ccacgaattc ttcggctgtg
     3541 tcggcacgca acaaaccctg ggcggcagtt gcgccaatgg cgtccgcgca actgctgacg
     3601 gtgaccaccg gctgctgcat ggccatggcc tccaggatct tgttctggat gccgcgcgcc
     3661 acgcgcagcg gagccaccac ggcgctggcg tgctgcaggt agggtcgcac atcgggcacg
     3721 gtgcccgtga ccaccacatt ggctccggcc agggccttca cctgcggtga agggctgcgg
     3781 cccacgatat aaaagcgcag gccggggtgc cgggcaagca ggcgcggcag catgtcggcc
     3841 acgaaccagc tcacgccgtc gatattcggc caatagtcca tggcgccggt gaaaaccagg
     3901 gcctgctcgc cctggtcaaa agggttggct agttgtgcgt cgggtttgaa gaaatcgcaa
     3961 tccaccccat tgcccatgga ttggacgcgg cccttggctt cgggtgcttg cgagacaaac
     4021 agggctgtct cattcggcgt cacaaaatac gagcaatgcg cctgcgcggc taccgcgcgt
     4081 tcataggcca ggagccgccg cccctcgcgc cggtacacca tcgacaacgg ccagcgatgc
     4141 gcgggcgcgt actgggtcca cttggcggag tccacatcaa caaaatcgac cagcatcggc
     4201 aggtccggca gcagcggctg ggcgtactgc gccatggcgg atgaaaacac gacacaagcc
     4261 tgcaatttgt gctcggccac ggtctgctgc acccaccgtt gcatgccggc atttctgtag
     4321 taggtcaggg tcagcggctc accggtcagc aggccgctca ggcttttgat cttggcgctg
     4381 cgcggctcaa tgtgctccac gtgcaagtcc ttgcacatgg cttgcaatac aggcaggtgc
     4441 tgcacatcgt ccgggtcgtc gataaacgtg cccagaaata ccctgtggtt cttctgcaaa
     4501 tgacgcagca ggtggtaaga gcgcaccttg tcccccttgt tgggcggata aggcagtcta
     4561 tgcaccagaa aaagaatgtt gctcacggcg gccatgcctt ccaaatgcct atccaagatt
     4621 gcgcacgatc ttgggcccga gccaattggc cagctgaatc ggcatgcggc gccaggtggc
     4681 gatcagcaac cgatacttgg cattcgcggg gttgttctgc ggcacgctgt cagttttgta
     4741 tagctgatat tcgtagtgca acggcgtggg ttcgaaaccc caatttttct tgaacgcata
     4801 cgaacccgta ttcagcttgc tgcgcccata gtcaaacacc ttcaggccac gcgcgcaggc
     4861 aagccgcatc agctcccaat acttgaagtc attggcggcg agctcacgcg cggcctctgc
     4921 atcgccagcg tagtaaggaa gcacctcatc gcgaaaataa aaactcagca cgctactcac
     4981 gggcttgcca tcgggagcgc acaccgtcaa cacttcgcag tcagggccaa atacgcgcag
     5041 caattgattg aaatacgcct tgggcatcgc cggcgtgccg tggcgatgga cgttgtcggc
     5101 atacagcgca aagaatcgat cggccgtggg atcgatctgc gacgtcaagc cattcttgat
     5161 gcccttgcgc accatcgcgc gttgtttgcg cggaatggcc agcagatttt tctcttcggt
     5221 tgcctcgatg ggcttgcgaa aggtgacgta gagatcctgc aggggccagt ccgcgtgccg
     5281 gcgctgcaca ttgcgcaact ccaggtgatc cacgcccagc tctcgtgcca gctgttgcgc
     5341 cctcagttcg agcgctgtag ccgtctcttc atcgaccgca gccacaccgc cgtaaaccga
     5401 gaatggcaag ctggtcagtg ccttgccaaa caaacggctt ttgacatgcc ccaggggcaa
     5461 taccccacgg ataactccgc cgctttccgc atacaaaaaa tagctgtcgt gaccaaaaat
     5521 atcctcgatc agtacctgcc agcccgagcg atgaaagaac gtagcctggg ggcaggccat
     5581 gacaaatgca tcccaccggg cgacatcgat gctattgcgc cgatcaagcc tttgaatgga
     5641 tagcgaggta acgacatcag acaagggaag ctttctgcac agattgaggt agaaatatat
     5701 gatccatccg cccccatctg aaatctcgta acaaacaccc cagacgttgc tccatgcgac
     5761 caatgttcac atagtgcctg aaccgtgtct tgaggcccac tccctccaca cgtggctgat
     5821 ttttgtcaat ttcccaaggg tgaaaataaa acactgccgg cgatgcatcc ttgctattga
     5881 cctggcgcag catccagcgc gacaaggcat agggtagcaa acggaaatac ccaccaccac
     5941 tcgacggaag attgcgattg aacatgcgca tggtggttgg tggcacctcc atcaacccat
     6001 cacgcgcctc gtaggcgaac cgcggcgcat ccggcatacc ataatgatca tgctgaatgg
     6061 ggtagatgct ggaactatag cgatagcctg cacgctgcaa ggtatcaagc gcccacaaat
     6121 tttgggagcc tatggaaaaa ctgggggcgc ggtaaccttg aacctccacc ccgccaatat
     6181 cttcaagcaa gctcttggcg ctgtgaatat cgtcaaaaaa ctgccgctcg gacagatcac
     6241 tcacccgctc gtgcccatag ccatgactgg caagctcatg cccgccatca acaatggcgc
     6301 gcaccagctt cgggtatctt tgcgcaatcc atccaagagt gaagaaagtg gcttgcacat
     6361 tgtgctgggc caacagtttc aatatttgat cgacattgcg ctcaaccctg cactctcgag
     6421 aatcccagtt gctgcgttga atatacggag caaatgccga cacctgaaaa tagtcttcga
     6481 catcaatagt caacgcattg gtgataggtg gtgtaatact catggcacca tctatccatt
     6541 gctggacacg ttgctatcag gtcgtggccg ccggcttgcc gccaccagtt tttgcagtgt
     6601 catcaatatt tccaggctgg cacgctcttg tcgcagcacg ctgcgttcta ttcgcaacaa
     6661 atgcgcgcgc agttgctcaa ttgtcatttg catgagctga tctgaaattc catcagccac
     6721 atctgcatcc aactcaatag agtccaagcc gtattcggca tcaacggatg tatcgtaggc
     6781 agaagatccc cccgtggctc tcagtggtgt attccacggt ggcaacgtct ttggcggaac
     6841 ctgcatctca gattgaactt cgtgaattat tccaatcacc agttcaacgt caatatgcga
     6901 tttgtcagcg aggaagccct gaagcagcac tctatccatc aaggtattga tgcggcgcgg
     6961 caccccgcca gcaaaccgat agatttcatc gaacacttcg tcatcaaacg ttggcttttc
     7021 tgcagcaccc gcacatttga gccggtgcaa aatgtattct cgcgtttcct ccgcatccag
     7081 ggggccgaga tgacaggtcg caatgacacg ctggcgcaat tgcagcattc caggactttg
     7141 caggatttct cgaaactctg gctgcccaac cagaaaggtt tgcagcaatg catcctgccc
     7201 gttctggaag ttcgaaagca tgcgcagctc ctcaaccgcc tgaggttgca ggttttgcgc
     7261 ttcatcaacc accagcaggc agcgcttgcc ctggagtgtc tgggccacga agaacgcctc
     7321 cagcgacatc agtaagtcgc ctttggacat cccattcact gggacaccaa acgctgcccc
     7381 caccagccgt agcgtgtcat ctgcccccaa ctgggtactg accaaattag cggccacgac
     7441 cgaatcggaa tcaaggctat cgagcaaacc acgcacgatg gtggttttgc ctgcacccac
     7501 ctcaccggtg atgacgatga acccctcgca gcacatgaca ccgtactcga gatatgctcg
     7561 agcacgctgg tgttgcttgc tactgaagta gaaacccgga tcaggattga gctgaaacgg
     7621 tttgctggtc agcccgtaga acgcttcata catgaatcaa aatcggtgcg taataaaacc
     7681 cataatcgcc gactcgccat actgactgac gtttccatca gaaagcacgc ggcgcaactg
     7741 aagcccaacg ctggtgcgtc gtgcgaccag acttgtccag gaaaatataa acggcctggt
     7801 ccgcgtctcc aatcccgtat caacacccga gttgcgcatt tcgccaaaac tgagagtgac
     7861 acctgagctt ggtgtaagac ggtgactcgc cgatatattc caaccccgtt gcctgatctt
     7921 gctgtcatta ctgaaatcat cgccaagcga gaaagcacct tgtagcagcc tgctatcaga
     7981 tcgcatcaca gccactgtca atgtactgcg ttgacccaat attgcgaatg ataaattttg
     8041 taatcgttgc agtttactgg acgatgtcaa aaaatcctgg aagacctgca tatccgcagg
     8101 caatcccatc cgctctatct ccgcctgcac caactgcata cgccgaatgg cattgggctc
     8161 ggttcgcata taaaatccgt ccagcagatc atatagaggg ctcggcgatc caaactgatc
     8221 tcccaatcca ttaacaacgg attttttatc cgtgtacctc catacagtac gccccgtgcg
     8281 atattgggca acggcattgt gcgactcgcc aaaataacgt tttacacgtt caaaagacac
     8341 gctggtacgc tctgacggcc gccagtctac gccaaatcca agaatactgc tggtttcacg
     8401 tattggtgac aactgattgg tggattctgc accgcctatc gccacaagac gcaaacccga
     8461 tctgggcatg tagcccaaac gcccgcgcaa ggccgttgta tctatatttc gcccattggt
     8521 atattcggca ttctgttgcg cggcttcgac agtccacgac atcaattgac ccaacggccg
     8581 tttttccaac tgaagaagcc agtcatgcac catcacattg gaacgcgctg cagtgtcgta
     8641 gcgcgtgtcc gtcatgcgat accgcgcctc atacgacaat ccatcagtta aatctccttt
     8701 gatgtaagga gaaacactaa atgatgtggc ctgcgtcaga ttggtgtttg cggtgttagc
     8761 gccgccagcg gggccgaatg cagatattgg ctgcaaagca gctcggccgt ccacatcgac
     8821 aaatacatgg tcttcgacgg cttccaccgt ccctctggca taaaggttat gccaaatttt
     8881 gtctggactg gtgtcacgcg catagttgac cgctcgcagc gtatagtcaa caaagccctt
     8941 gaggcgcgcc gtgtcccttg ccaagcgaaa acctggcatt acttcggtta tctggtcgcc
     9001 gctgccgttg tggataaggc gtgaattatt ggtaacggtg tgctgcaccg aaatgcgtgg
     9061 ctcgaaccac tggcctgacg cccgcggctc cggttgatca ctaccagacc gcgccgccgc
     9121 gccactaccc gctggcgact gcgtggaagc agtacgggat tgtggatcgg aacccagatc
     9181 aggaacctgc gctgcgacca gcccaggagc ggaggccaac acccatgccg cccaaccaat
     9241 cgcgggaatg cgtaccaaac ctacatgcgg caaatacatt tcagacgtcc tctttctatg
     9301 cggccgctgc cggcatgcgc tcttccgtcg cccttccata accgccatgc ccatagccat
     9361 agccatagcc atgtgcaccc aggccctggg agcgcacctg attcagcacc agaagcttga
     9421 tggggcaggc ctcgatggtg gacagcgcat gctccacttc tgactgcagt gttctttctg
     9481 ctcgaaccac aacgacgacc tgccccatgt gattggcgag aacgcgcgcc tctgtcgtca
     9541 gcaacaatgg cggcgaatca aaaataatga tgcggtcgct ataacgcttg ctcatttcgt
     9601 cgagcaagtc ggtcattgcg tcactggcca gcagctcggt tgcgcggtcg tggggtaaac
     9661 cggcgggcag cagggtcagc ccctccacgt tggttcgcag aagcacgtcg gccagctttg
     9721 tttgtccgcc ccgcagcatg tccagtaatc ccgcggcagg cggcaggccc agcttggaca
     9781 tcactgaggg acgagtgaca tcggcatcca ccagcatgac ggtgtgatcc agctccatgg
     9841 ccaggctcat ggccagattg atagccgtaa agctcttgcc ctcccctggc aaggcgctgg
     9901 tcaccatgat caggttgccg tttgtcacca aggatgcgcc cttgcccatg gcattttcca
     9961 ggagtgggcg tttgatcaca cggtactgat ccgtcgtgac cgagcgcgga gagccaggag
    10021 tcacaaaacc gctggcactc agggctgtca aatcaagctg cacggttttc gaaaccgccc
    10081 tcagttcagg agctgcatga tcgccatcgg ctgcaggcgc agcaaactgc ggcacgtgat
    10141 tcaagggctg ggattcatct ggcacgcccg ggttctgcac ccgtggtttg tcgccgggca
    10201 acaccgcgcc cgccttgcgc agttgttcca gcctctgggc tgctttttca attgagctgg
    10261 tcatgattgc ttgtcacccc aaggcacctg tacgaatgga caatgcggcc ataccgcccg
    10321 cgtaaagcag caaaaatgca acggtcgcca tgatgaagcg cttcaagctg cgcgactcgc
    10381 gtcggcgctg ctcttcgctc gggatcagcc ccaccacccc cagcaatggc aactgggtca
    10441 cctgccgcaa ggtggcgccg tcaaagaaca ccggcctgat ctgactcatc acaaaggcaa
    10501 tgccaagccc cgctgcgacc gccgccagca ttgctcccgg catcaacagc aggcgattgg
    10561 gtgcaacagg cttgggggcc acacgcggag gatcgatgac gcggaattcg gccacattgg
    10621 aagtgttttc caactttccc gacatgagag cggattcacg gcgcgccacc agatcggcat
    10681 agttcttttg atgaatttga tagtcacggt tgagttgtgc cagctctgcc tcgagctgcg
    10741 gcgccacctt cagttgctgg tgaacacgtt gcacacgcgc ctcatactca accacgcgcg
    10801 cgcgcagtga agccacctgc acctgggctg cggaataaat gcggctcagc tccacggctg
    10861 cgggattgac ctgggcaatg ggcacctcgc cggtttgctg gagcttgcgg cgcaatacgt
    10921 cgatttcctt gcgcttttgc ccttccagat cggcgatcag cgtccgcgtc cggatgacgt
    10981 cggggtgacc gtcggtgtag cgctgcagca gtgcgtccag gttctgcttt tgcgcaagca
    11041 agcgtgcatc gagctcaggc gtctgcacgt cgggcgccgg gcccgccatt gatttggcgg
    11101 tttgcgcacg catttcttcc agctgctgtg ctgcggcagc cctagcgctt tcggcttcac
    11161 gcaactcaag ccgtgcctgg ctcagagtgt tttctatctg ggaggcacgc ccggcggaat
    11221 ccagcccctc ctcagactgc atctcaatat tgcgcagctt gaatgccttg aggcgccctt
    11281 ctgcctcggt cagtttggtc tcgtaggtct tgatctggtc gtccagaaac cggcgtgcgc
    11341 tgtctgaatc ctggcgcgtt gctcccaggc tggattccac gaaaatggtc aacagcgact
    11401 tcaccacgag ttgcgccttc tcagggctct ggtcgcggta ggacaaggtg tagaggttgt
    11461 cacgccccgt ggattggatc ttgatggact gggtgacagc atccaccacg gcatccgttg
    11521 aggccttggt cgtggagccc agatcaaggt cggccatgcg caccagccgc tccacggtcg
    11581 gccggttgat cagcgtgcgg ctcagcatcg acacctgctg ctcgatattg ggctgcacgg
    11641 caataccggt catgagtggt cgcaagatgg actgcgtatc cacgaacacg cgcgccgaag
    11701 cctggtaata gtccggcatc gtgagtacca cgccagcgcc tatcgcacca accacccata
    11761 ttgtcagcat cgccagccgg cgatagatcc acatgccacg cgcagtggta gtgatctgac
    11821 ttagaagctc atccattcca ctccccttga ccaactcatc agcgactcac tagcgagcag
    11881 gccgattaaa accacccttg aggaatgatc agaacatcac cgggcaacac ttcaacattg
    11941 gcatcaatgt caccgcgctt gatgaggtcg tgcaaacgca ccttgtagcg cttgttgtcc
    12001 tcggccgcgc gaaccagggt ggcgctgttg ccgtcggcaa aatcggtcag gccgccgacg
    12061 gcaatcatca cgtccagcac cgtcattttt tgcttgtatg gcaggaattg cggcttggcc
    12121 gcctcgccca ccacacggat ctgttcactg tacggaccaa cgaagttggt gactatcacc
    12181 gtgacaaccg ggtcgcgcac gtacttggcc agctctttct cgatgtcacg cgcgatctgt
    12241 gccgagttct tgccctgcgc ctgaaggccg tcaatcagcg gcaaggacac ctggccgtcc
    12301 gggcgcaccg gcactgacag cgaaaggtca ggattgcgcc agacgatgat gttcagggta
    12361 tcgccaggcc ccacgacgta gttgtagtcc gggctcgacg ccttgaccgg cgccgcaggg
    12421 tagttgccgg tacccgcaca gcccacggca gtcacagcca ctgcaaccac ggccgcagcg
    12481 cgacccacag aaccgacgag ccttgaaaag aatccacgca tggtacaact ccgatacata
    12541 tttaacaggt gatttcatca aatgccgctg cagcataccg tggatgaccg cagcacgtaa
    12601 atactctgat taagggatac caaaccgccg gctgtgccag ccatgcaccc ctgcggcgtc
    12661 tgaaccggat cagcgcccat acacatcccc cagacgcacg atgtcatcct cccccaggta
    12721 ggagccggac tgcacctcga tcatctccag gtctacatgg ccggggttct ccagccggtg
    12781 cgtgacgccg agggggatat aggtggactg gttctcggtg agcaaaaaag cctcgtcacc
    12841 cttggtcacg cgcgccgtgc cgcgcaccac gacccagtgc tcggcacgat ggtggtgcat
    12901 ctgcagcgag agcttgccac caggcttgac catgatgcgc ttgacctgga agcgctcgcc
    12961 cgcgtccaca ccgtcgtacc agccccaggg gcggtacacc ttgcggtgca gcatgccctc
    13021 ggcgcagccc tcgcgcttca ggcgatcgac gatcttcttc acgtcctgag tgtggcgctg
    13081 atcgaccacc agcaccgcat ccggcgtctc caccaccacc aggctctgca cgcccacgca
    13141 ggccagcagc cggcccgaag aataggccag ggtatcgttg cagtcctgca acatcacccg
    13201 cccctgactc acgttgccgg cctcatcctt gggcagtatc tgccacagcg catcccatgc
    13261 gcccacatcg gaccagcccg cggacagcgg cagcaccacg ccctgcggca agccctccac
    13321 cgcctgcgct ccggccaggc gctccatcac cgcgtagtcg atcgaatcgc tggggcaggc
    13381 ggcaaaggcc tcggcatcca cccgcacaaa cgacaggtcg gaggtgctct tgttccaggc
    13441 cgcctcgcag gcctcgagga tgtctggccg gcatagccgc agcgcattca gccacacgga
    13501 cgcccgcagc acgaacacgc cgctgttcca tgaatagttg ccctgctgca ggtagccctc
    13561 ggccgttgcg cgatcgggct tttccacgaa gcgggcgatg gcacaggcac caccggcatc
    13621 cagcgccgca cccgcctgta tgtagccata gcccgtctcc ggtcgatccg gggtgatgcc
    13681 aaaagtgacc acggctccgt tctgggcaag gcgcgcggcg tggcgcacgc attcctgaaa
    13741 tgccgccacc tggctcacca catggtcggc cggcatgacg agcaacaccg ggtcgtcgcc
    13801 agccgcccga gcagccagcg cggccagcgt cagcgccggt gcggtattgc gccccacggg
    13861 ttcgagcacg atgcggccct gcagccccat cagcctgagc tgctcggcca ccacaaagcg
    13921 gtactcgtcg ttgcagacca ccagcggcgg cgcgcaggtg gcgccatcga tgccctccag
    13981 ccggcgcaac gtggcctgca acagcgaatc ctgccccacg aaggaaagca gctgcttggg
    14041 gtacttttca cgggacagcg gccacaggcg cgtgcccgaa cctcccgaca aaacgactgg
    14101 ctgaatcaac atgatgtgct ggcaatccgc ttttttgaca ggcaagaggg taatccgggt
    14161 tcatggcgac ccgtcagcga tcgaaccctg atgcccatgc cgccagagtt tggcttttcc
    14221 tatcggcaat gtagattgat tgtttgttgc gatgcagcat acctgatcgc tacgcggccc
    14281 aactcctata tcgaccgctt ctggcataaa tggaaacaat ttcacgattc gagcctgtat
    14341 gcccacgcct ggctgcaacc cattgacttg taaagagaca caaatcattt tccgagcggg
    14401 ggcgccggtc attcgatcgt cacgctcttg gcaaggcttc cgggcttgtg ccgccacata
    14461 acttcgctgc gcgaagtgag tggcgtccaa accgcgccat ttgggcgcta cgcgcggcgg
    14521 ctgcgccgcc ccaagagcgc gccggtcatt cgaccgtcac gctcttggca aggcttccgg
    14581 gcttgtgccg ccacataact tcgctgcgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnc ggcggctgcg ccgccccaag agcgcgccgg tcattcgacc gtcacgctct
    14761 tggccaaatt gcgcggtttg tccacatccg tgccgcgggc gcaggcggtg tggtaggcca
    14821 gcagttgcag cggcacgaca tgcagcaaag gtgacagaga gccgtaatgc tcgggcatgc
    14881 ggatcacatg cacgccctcg ctgctctcga tattggtgtt ggcgtcggcc agcacgtaca
    14941 gcacgccgcc gcgcgcgcgc acttcctgca tgttgctctt gagcttttcc a
//
LOCUS       DAOMTQ010000068        14928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_637_length_14928_cov_25.043031, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000068 DAOMTQ010000000
VERSION     DAOMTQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_637_length_14928_cov_25.043031"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK518_08330"
     CDS             complement(<1..413)
                     /locus_tag="PK518_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRM48961.1"
                     /translation="MEDIVIVSAVRTPVGKFGGALAKTAATDLGAIVIKEALARAKVG
                     LDQVGEVIMGQVLAAGCGQNPARQALMKAGVAKETPALTINAVCGSGLKAVMLAAQAV
                     ATGDSEIVVAGGQENMSLSPHVLNGSRDGQRMGDW"
     gene            complement(471..2180)
                     /gene="phaC"
                     /locus_tag="PK518_08335"
     CDS             complement(471..2180)
                     /gene="phaC"
                     /locus_tag="PK518_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRM48962.1"
                     /translation="MSNDAAWAESVQQFQQMLSQSWGNMLQAMQPGELNKAMAMPPST
                     PVNFAGDKLLELQQQYLQDMRALWGQGMLPQMPKTGDRRFAGENWAQNPLSVYSVTAY
                     QMQARALMGMVDAVEADEKTRARIRFGVEQWLAAMSPSNFLAFNADAQKKAIETHGES
                     IAKGVANLLHDMRQGHISMTDESRFEVGRNVATTEGAVVFENELFQLLEYKPLTAKVY
                     ERPFLMVPPCINKYYILDLQPENSVIRYAVSQGHRTFVISWRNPDESLGHKTWDDYIE
                     QAVLTAIAKTQEISGVEKINALGFCVGGTMLTNALAVLAARGQDVVASATFLTTLINF
                     ADTGILDVFIDENFVRMREMQMGQGGLMKGQDLASTFSFLRPNELVWNYVVGNYLKGE
                     TPPPFDLLYWNSDSTNLPGPYYAWYLRNFYLENKLIEPGALTVCGEQLDLRQVKLPVY
                     IYGSREDHIVPADAAYASTQVLPGKKRFVMGASGHIAGVINPPAKNKRSHWVREDGKL
                     PATLQGWLAGADELPGSWWDDWSEWLKGHAGKQIAAPKDYGRAPEFKAIEPAPGRYVK
                     QKA"
     gene            2340..2624
                     /locus_tag="PK518_08340"
     CDS             2340..2624
                     /locus_tag="PK518_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48963.1"
                     /translation="MYLVLIAWLYVTVMMAVAEATSAQGTLLGAGITFVLYGLLPIAI
                     VGYILGTPARKRAIRAREQALAASAADPDAGSHAPAGAQDGAVAPVRKEP"
     gene            complement(2541..3380)
                     /gene="pgeF"
                     /locus_tag="PK518_08345"
     CDS             complement(2541..3380)
                     /gene="pgeF"
                     /locus_tag="PK518_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRM48964.1"
                     /translation="MTHRMPHDWLQPDWPAPAHVHALCTTRNGGVSAPPWDSFNLGRY
                     VGDAPEAVAANWAALQAAVSARGQPVRPVYLRQVHGTALAALQADTPDGGDADACATR
                     VPGVACTVMAADCLPVLFTNRAGTQVAAAHAGWRGLAAGVLEAAVQSFQAESLVDKAH
                     QATTNIAHQAPQELLAWMGPCIGPQAFEVGPEVREAFCAHDPQAAACFKPHGRAGKLL
                     ADLAGLARQRLRAMGVDAIHGNDSSPAWCTVANPSRFFSHRRDSPVLGTSGRMAACIW
                     ISG"
     gene            complement(3385..4053)
                     /gene="maiA"
                     /locus_tag="PK518_08350"
     CDS             complement(3385..4053)
                     /gene="maiA"
                     /locus_tag="PK518_08350"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRM48965.1"
                     /translation="MKLYNYFRSSASFRVRIALELKGLPYDYVAVNLLKGEQLLPAYA
                     SQLGDALVPALELDDGRLLAQSMAIIEYLDETHPAPALLPGDPIARAHVRALAQLVAC
                     EIHPLNNLRVLKYLKRQLQLDDAAKDAWYSHWARQGLQAFERQLLLLDAERKATGQAP
                     SRLCWGDQPTLADCCLVPQIFNAQRFHVELHDLARTMAAFQACMALPAFQRAQPSACP
                     DHEG"
     gene            4132..4827
                     /locus_tag="PK518_08355"
     CDS             4132..4827
                     /locus_tag="PK518_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRM48966.1"
                     /translation="MSYVIPPAAPASVPVVGRAERFAVHRIYCVGRNYAEHAREMGFT
                     GREPPFFFMKPADAVLPVAQGETGHLPYPTLTQDLHHEIELVVAMGKGGRNIAAGDAM
                     QHIWGYAVGLDMTRRDLQAEAKKQGRPWCIAKGFDASAPIGPITPAAQAGNVQQAAIW
                     LQVNGADRQRSNVSQLIWNIAETIEHLSQAWELRPGDLIFTGTPEGVSAVVRGDVLEG
                     GIDGLCPLRVAVA"
     gene            4902..5450
                     /locus_tag="PK518_08360"
     CDS             4902..5450
                     /locus_tag="PK518_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM48967.1"
                     /translation="MIFRRPIKHCRECGAAVTYRLPDDGDTRARAICPACNTIHYENP
                     LMVVGTVPVLGERVLLCKRNIEPRWGKWTLPAGFMELGETAEQGAARETDEEAGALIT
                     MGPLFSLLSVPQVGQVHIFYLATVLSERFDPGHETLEARLFAEDEIPWDEIAFRTVKR
                     TLECFFADRRGGQFGVHSYDIL"
     gene            complement(5447..7885)
                     /locus_tag="PK518_08365"
     CDS             complement(5447..7885)
                     /locus_tag="PK518_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRM48968.1"
                     /translation="MVFFDSLRARTAGLCAALVRRPDSWLSAHGLLWLLAVLALAVVL
                     YVLALIPFTPAISDIRKAKSDQPAQLVSSDGRLLAEYRWVNREWVPLSTIARPVVDAL
                     IATEDHRFYEHFGLDWRRTASAMVRTFGGERQGGSTITQQLARNLYPEDIGRAPTLTR
                     KIKEAITALKIEWLYSKDEILETYLNTVPFLYNAYGIEMAARTYFDKSADRLTVLESA
                     TLVGMLKGTAYYNPVLNPERAQERRNTVLAQMKKRGKLASAEYDKLAKRPLRIRFERQ
                     SELSGLAPHLAQQLRRQLIDWADRNGYSLYSDGLVIRTTIDGRLQEMANQAVQRQGRQ
                     LQGVADAAWAPRAAWSARSPLVQQLVRESPQYDKAVAAGATPEEALQQLLADGDFLRR
                     LRAAKTRVQAGFLALDPRSGAVLAWVGSRDFEQDPFDHVQAARRQPGSTFKPFVYGAA
                     FAKGMLPTDQLMDQAVEIPLGGKQVWRPTDGRTPPSNEPMQLRDGLAYSKNTITAQLM
                     QQVGPQQVAQLARAMGVRESRLEAVPALALGTSAVTLKEMVAAYGTIASGGLYRAPTV
                     ITRIEDKHGKLLEAFQSAAPERALGLAAAQQLRDALRGAVDKGTGVAIRARYGITADV
                     AGKTGTTQDNTDGWFILMHPQLVAGAWAGFNDGRITLRSDYWGQGAHSALPMVGDVFR
                     QALRGKIIDARERFIDEEESSWIATATGTLRNWVYDLFGRPTGQQASPAAVPAPEAPR
                     APVPAASAPPQDERPEITEAPLVPLQEVAPRAPAADPPVWVDQPDAQPVQPYAPPPAM
                     DSAALQPATFNLDR"
     gene            complement(8069..9004)
                     /gene="rssA"
                     /locus_tag="PK518_08370"
     CDS             complement(8069..9004)
                     /gene="rssA"
                     /locus_tag="PK518_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HRM48969.1"
                     /translation="MALARREPKVGLALGSGSARGWAHIGVLQVLRDEGVHLDMVCGS
                     SIGALVGAAYAAGELERFAEWVQTLGLREVFGFMDFNLAGGMLKGEKLIAFWRRHFAD
                     FDIDASPLPFACVATDLHSGAEVWLRDGSIADAVRASIALPGLFTPVVHADGRLLVDG
                     GLVNPVPTSLARAMGADIVIGVDLNADIVQRHMHPLAVVRESQPGDGPEALPAPEPAH
                     NGDWMRRLKFWAGSNGKAPAPKQPSMLDVMMTSVNIMQMRITRSRMAGDPPEVVIAPR
                     LSHLGQLDFHRAQEAIDEGRRAAQASLPQLRQFMG"
     gene            complement(9007..11091)
                     /gene="ligA"
                     /locus_tag="PK518_08375"
     CDS             complement(9007..11091)
                     /gene="ligA"
                     /locus_tag="PK518_08375"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRM48970.1"
                     /translation="MTESLDLFSAPAPAAPALVATKIENLRAQLNRWAHEYYVQDAPS
                     VPDAEYDRAFQQLQALEGAYPGLVTPDSPTQRVIGAVMDGLASVRHSVPMLSIQTETD
                     TEASGAEAFDARVRRELGLDASAPPMEYVAEPKFDGLAMSLRYEHGRLTQAATRGDGE
                     VGEDVTHNIRTIRQIPLTLPADVPPVLEVRGEVYMRRADFGALNERQRERGEKTFVNP
                     RNAAAGAVRQLDSGITAQRPLSFFAYGLGEVTPAEQGGPDFGTHYALLQQLKTWGFPV
                     APQVQIALGASELIAFHRRLGAERDALPYDIDGVVYKVNSLALQRQMGFKTREPRWAV
                     AHKYPAQEMLTRCEGIDVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFEIRKKGV
                     RVGDQVIVRRAGDVIPEVVGVVAGQRPAYVPNFRMPRQCPVCGSAVVREAGEMNHRCS
                     GGLFCPAQRKQALLHYAQRRALDIEGLGEKLVDQLVDGELVRSLADLYRLDLPTLAPL
                     ERMAEKSAQNLLDALQKSKRTTLPRFLFGLGVRHVGEATAKDLARHFGSLDAIMNAGV
                     EQLLQVNDVGPVVADSIHTFFAQPHNREVVAALRAHGVTWEEGAPAEHTALPLAGKTV
                     VLTGTLPTLSRDAAKDLLEAAGAKVAGSVSKKTHYVVAGAEAGSKLARAEELGIPVLD
                     EDGLHALLRGAL"
     gene            complement(11239..12342)
                     /locus_tag="PK518_08380"
     CDS             complement(11239..12342)
                     /locus_tag="PK518_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HRM48971.1"
                     /translation="MSNFQLSLIIIGALVLIVVIAYNAWTTRRNTPKRASPREGDKGQ
                     EPAVRQEPAFDGAGLGQVDAAQASAAEPHFEGHAVDARDALDLPVPQPERRGGLDPLI
                     DVIAPIVAEQQVSGDAALAALPPTRRAGSKPFAIEGLNLASNQWEQPQPGQRYVQFQA
                     GVQLANRVGALNEIEFSEFVMKAQAFADAINATPDFPDMLHEVARARELDQFASDHDA
                     QLSFMLRARQAAWSPGYVQQNAARCGFVAGAIPGRLVLAASGVGLPPMLTLGYDSQAA
                     MSDDPEQSAVRDIMLSLDIAQVHRSEQPFARLREVAAQLAEAMDGVLCDQNGTPLPAM
                     TMEPIAADLEQLYDQLDSHELSAGSVLARRLFS"
     gene            complement(12354..>14928)
                     /gene="smc"
                     /locus_tag="PK518_08385"
     CDS             complement(12354..>14928)
                     /gene="smc"
                     /locus_tag="PK518_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRM48972.1"
                     /translation="EQRLLQLAEQIQQWSARKQEAEAEAEHLAGAGENAEEQAELLAA
                     QVEEQAMQLPDLEDALRQAQQRSEQQRSAVVQIQQQIQVLAAEQRSLDEQSRQLDARH
                     ERLRSDRNALAAPDEARLANLRQQLQEAEEGAELAAARQEELQDSVPQLDEERRTRQQ
                     TVNHEAARQADLSARLDALKALQEKLKVDGKLQPWLARHGLDGLQGLWSRIHIEPGWE
                     NALEAALRERLAALPVGRLDMVRGFLGAGGADAPPARLAFYSAPATPQPEPTATQPRL
                     SDLLRVDNGGLRAVLVDWLHGCFTAQTLDEALDRRAQLQPGQAIYVAAGHAVAAHSLS
                     FFAQDSEQSGLLARAQEIEHLEKELRAQQLIAEEARTALVRAEAAYADASQRLVAARR
                     EASEAQGRAHELQVQTLQLTQLAEQARARGAQISADLAEVEAQLSDLQERRVQSEARF
                     EELDMQLADSQERHAQLGDRVIEAERRLNECREQQRQLERRVQEAQFSQRSLQARRAE
                     LARTMDTAATQARTLQDERQRAQDELARLSDAAAQGGLQQALDLKLEREKLLGAQRSQ
                     YDELTARLRASDERRMQHERQLDPLRARITEFQLKEQAARLGLEQYSSLLQDAQADLQ
                     AVALSIEEGGVRAAGLQGEIDRLQREITALGAVNLAALEELTLARERKGFLDAQTEDL
                     TLAMTTLEDAIKKIDGETRSLLQGTFDAVNGHFGRMFPELFGGGQARLIMTGDEILDS
                     GVQVMAQPPGKKNQTIHLLSGGEKALTAIALVFAIFQLNPAPFCLLDEVDAPLDDANT
                     ERYAKLVSAMSRGTQFLFISHNKIAMEMAEQLIGVTMQEQGVSRIVAVDMQSALSMAE
                     A"
BASE COUNT         2198 a         5083 c         5132 g         2515 t
ORIGIN      
        1 ttccagtcgc ccatgcgctg gccgtcgcgc gagccgttca ggacatgggg cgacaggctc
       61 atgttctcct ggccgccggc gacgacgatt tcgctgtcgc ccgtggccac ggcctgggcc
      121 gccaacatga cggccttcag gcccgagccg cagacggcgt tgatggtcag cgccggggtt
      181 tccttggcga cgccggcctt catcagcgcc tggcgcgccg ggttctggcc gcagccggcc
      241 gccagcacct ggcccatgat gacctcgccc acctgatcca gccccacctt ggcgcgtgcc
      301 agggcctcct tgatgacgat ggcgccaaga tccgtggccg cggtcttggc gagtgcgccg
      361 ccaaacttgc ccacgggggt gcgtacggcg gaaacgatga cgatgtcttc catgtgagtc
      421 ctctctgaag taatgcccgg cagcgccttg cgccgggcgg atgaatccga tcaggccttt
      481 tgcttgacgt aacgcccggg tgcgggctcg atggccttga attcgggcgc cctgccgtaa
      541 tccttggggg cggcgatctg cttgccggca tggcccttga gccattcgct ccagtcatcc
      601 caccagctgc cgggcagctc atcggccccg gccagccagc cttgcagcgt ggcgggcagc
      661 ttgccgtcct cgcgcaccca gtggctgcgc ttgttcttgg ccggggggtt gatcacgccg
      721 gcgatatggc ccgaggcgcc catgacgaag cgcttcttgc ccggcagcac ctgggtggat
      781 gcgtaggcgg catcggccgg cacgatatgg tcttcacgcg agccgtagat gtagaccggc
      841 agcttgacct ggcgcagatc cagctgttcg ccgcacacgg tgagggcacc gggctcgatg
      901 agcttgttct ccaggtagaa gttgcgcaga taccaggcgt agtacggccc gggcaggttg
      961 gtgctgtcgc tgttccagta cagcaggtcg aacggaggcg gcgtctcgcc cttgaggtag
     1021 ttgccgacga cgtagttcca gaccagctcg ttggggcgca ggaagctgaa ggtggaggcc
     1081 agatcctgcc ccttcatgag gccgccctgt cccatttgca tctcgcgcat gcgcacgaag
     1141 ttttcgtcga tgaaaacgtc gaggatgccg gtgtcggcaa agttgatcag cgtggtgagg
     1201 aaggtggcgc tggccaccac atcctggccg cgcgcggcca gcactgccag ggcgttggtg
     1261 agcatggtgc cgcccacgca aaagcccaga gcgttgatct tttcgacgcc ggagatttcc
     1321 tgcgtcttgg cgatggcggt gagcacggcc tgctcgatgt aatcgtccca ggtcttgtgg
     1381 cccagcgact catcggggtt gcgccagctg atcacaaagg tgcggtggcc ctgcgatacg
     1441 gcgtagcgga tgaccgagtt ctcgggctgc agatccagga tgtagtactt gttgatgcag
     1501 ggcggcacca tgaggaaggg gcgctcgtac accttggccg tgagcggctt gtattcgagg
     1561 agctggaaca gctcgttctc gaacaccacg gcgccttcgg tcgtggccac gttgcggccg
     1621 acctcgaagc ggctctcgtc ggtcatggag atatggccct ggcgcatgtc gtgcagcagg
     1681 ttggccacgc ccttggcgat gctctcgccg tgggtttcta tcgccttctt ctgcgcgtcg
     1741 gcattgaagg ccaggaagtt gctgggcgac atcgcggcca gccattgctc gacgccgaag
     1801 cggatgcgtg cgcgggtctt ttcgtcggcc tccacggcat ccaccatgcc catcagggcg
     1861 cgcgcctgca tctggtaggc cgtgaccgaa tacaccgaca gcgggttctg tgcccagttc
     1921 tcaccggcaa agcgcctgtc gccggtcttg ggcatttgcg gcagcatgcc ctggccccac
     1981 agcgcgcgca tgtcctgcag gtactgctgc tgcagctcca gcaacttgtc gccggcaaag
     2041 ttcaccggcg tgctcggtgg catggccatg gccttgttca gctcccccgg ctgcatggcc
     2101 tgcagcatgt tgccccagct ctggctgagc atttgctgaa attgctggac actttcggcc
     2161 catgcggcgt cgttgctcat acggtctcca tgaatatgcc cggaagcagg cacaggtggt
     2221 cattgttgcc gagcagtccc cggccacaca agagtcctca gcagtgctaa agggttttgc
     2281 atgcgcgtgg cagcgacgtg gcagttcaca atagggggct attttgcttg aagtcaatca
     2341 tgtatttggt gttaatagcc tggctttatg taaccgtgat gatggcggtg gccgaggcca
     2401 cgtcggccca gggcacgctg ctgggcgcgg gcatcacctt tgtgctctat ggcctgctgc
     2461 cgatcgccat cgtgggctac atcctgggca cgcccgcgcg caaacgcgcc attcgcgcgc
     2521 gcgaacaggc cctggccgca tcagccgctg atccagatgc aggcagccat gcgcccgctg
     2581 gtgcccagga cggggctgtc gcgccggtgc gaaaagaacc gtgacgggtt ggccaccgtg
     2641 caccaggccg ggctgctgtc gttgccatgg atcgcatcca cgcccatggc gcgcaggcgc
     2701 tggcgcgcca ggcctgccag gtcggccagc agcttgccgg cccggccatg gggtttgaag
     2761 caggcagcgg cctgcgggtc atgggcgcaa aaggcctcgc gcacctcggg gccgacctcg
     2821 aaggcctgcg ggcctatgca gggtcccatc caggccagca attcctgtgg cgcctgatga
     2881 gctatatttg ttgtagcttg atgcgcttta tcaacaaggc tttcagcctg aaatgactgt
     2941 acggctgcct ccagcacgcc ggcggccagc ccgcgccagc cggcatgggc agcggccacc
     3001 tgtgtgccgg cgcggttggt gaacagcacg ggcaggcagt ccgcggccat gacggtgcag
     3061 gccacgcccg gcacccgtgt ggcgcaggca tcggcatcgc cgccgtcggg cgtgtcggcc
     3121 tgcagggccg ccagcgccgt gccatgcacc tggcgcagat acaccgggcg caccggctgg
     3181 ccgcgcgcgc tgacagccgc ctgcaatgcc gcccagttgg cggccacggc ctcgggcgcg
     3241 tcgcctacgt agcggcccag gttgaagctg tcccagggcg gcgcgctcac gccgccattg
     3301 cgcgtggtgc acagagcgtg cacatgggcg ggcgcgggcc agtcgggttg caaccagtcg
     3361 tggggcatgc ggtgcgtcat ggcgtcaccc ctcatggtcc gggcaggccg aaggttgggc
     3421 acgctggaac gccggcagtg ccatgcaggc ctggaaagcc gccatggtgc gcgccaggtc
     3481 atgcagttcg acatggaaac gctgggcatt gaagatttgc ggcaccaggc aacagtcggc
     3541 cagcgtgggc tggtcgcccc agcacaggcg cgagggagcc tggccggtgg ccttgcgctc
     3601 ggcgtcgagc agcagcagct ggcgctcgaa ggcctgcagg ccctggcgtg cccagtggct
     3661 gtaccaggca tccttggcgg cgtcgtccag ctgcagctgg cgcttgaggt acttgagcac
     3721 acgcagattg ttcaaggggt ggatctcgca ggccacgagc tgcgccaggg cgcgcacatg
     3781 ggcgcgggcg atggggtcgc ccggcagcaa tgcgggcgcc ggatgggttt cgtccaggta
     3841 ttcgatgatg gccatggact gcgccaacag ccggccgtcg tccaactcaa gcgccggcac
     3901 cagcgcgtcg cccagctggc tggcgtaggc gggcagcagc tgttcgccct tgagcaggtt
     3961 caccgccaca tagtcgtagg gcaggccctt gagctccaac gcaatgcgca cgcgaaacga
     4021 ggccgaggag cgaaagtagt tgtagagctt catgccccgc agcataagcg ccgcgggccc
     4081 atggcacact gcgacggttt cgcccgcaac accagcgtca gaggtttctc catgagctat
     4141 gtcatccccc ccgcagcccc ggccagcgta cccgttgtcg gccgcgccga gcgttttgcc
     4201 gtgcaccgca tctactgcgt gggccgcaac tacgccgagc acgccaggga gatgggcttt
     4261 accgggcgcg agccgccgtt cttcttcatg aagccggccg acgccgtact gcccgtggcc
     4321 cagggcgaga ccggccacct gccctacccc acgctgaccc aggatctgca ccacgagatc
     4381 gagctcgtcg tggccatggg caagggcggc aggaacatcg ccgcaggcga cgccatgcag
     4441 cacatctggg gctacgccgt gggccttgac atgacgcgcc gcgacctgca ggccgaggcc
     4501 aagaagcagg gccgcccctg gtgcatcgcc aagggctttg acgccagcgc gcccatcggc
     4561 cccatcacgc cggcagccca ggcggggaac gttcagcagg ccgcgatctg gttgcaggtc
     4621 aacggcgcag accgccagcg cagcaacgtc agccagctga tctggaacat cgccgagacc
     4681 attgaacatc tgtcacaagc ctgggagctg cggcccggcg acctgatctt caccggcacg
     4741 cccgagggcg tgagcgcggt ggtgcgcggc gatgtgctgg agggcggcat cgacggcctg
     4801 tgcccgctgc gcgtggccgt ggcctgaccc agtttactat attaaatata gctgttcgcg
     4861 cttgatacat gagcgctaga gccaattttt aataacatag catgatcttt cgccgcccca
     4921 tcaagcattg ccgcgaatgc ggcgcggccg tgacctaccg cctgcccgac gatggcgaca
     4981 cgcgcgcacg cgccatatgc ccggcctgca acaccatcca ttacgagaac ccgttgatgg
     5041 tggtgggcac ggtgcccgtg ctgggcgagc gcgtgctgct gtgcaagcgc aacatcgagc
     5101 cgcgctgggg caagtggacg ctgccggccg gcttcatgga gctgggcgaa acggccgaac
     5161 aaggcgccgc gcgcgagacc gacgaagagg ccggcgccct gatcacgatg gggccgctgt
     5221 tctccctgct cagcgtgccc caggtggggc aggtgcatat cttctacctg gcgacggtgc
     5281 tcagcgagcg cttcgacccc ggccacgaga cgctggaggc gcggctcttt gccgaggacg
     5341 aaatcccctg ggacgagatc gcctttcgga ccgtcaagcg cacgctggaa tgcttttttg
     5401 ccgaccgccg cggcgggcag ttcggcgtgc actcctacga cattctttag cgatcgagat
     5461 tgaaagtcgc cggctgcaag gcggccgaat ccattgccgg cggcggcgcg tacggttgca
     5521 ccggctgcgc atcgggctgg tccacccata ccggcggatc ggccgccggg gcgcgcggtg
     5581 ccacttcctg cagcggcacc agcggtgcct cggtaatctc gggacgctcg tcctgcggcg
     5641 gggcgctggc cgcaggcacg ggggcgcggg gcgcctcggg cgcgggcacg gcagccgggc
     5701 tggcctgctg gcctgtgggg cggccaaaca ggtcatagac ccagttgcgc agcgtgcccg
     5761 tggccgtggc gatccagctg ctttcctcct cgtcgatgaa gcgctcgcgc gcatcgatga
     5821 tcttgccgcg cagcgcctgg cggaacacat cgcccaccat gggcagtgcg ctgtgcgcgc
     5881 cctggcccca gtagtcgctg cgcagcgtga tgcgcccgtc gttgaagccg gcccaggcgc
     5941 ccgccaccag ctgcgggtgc atcaggatga accagccgtc ggtgttgtcc tgcgtggtgc
     6001 cggtcttgcc ggccacgtct gcggtgatgc cgtagcgcgc gcgtatcgcc acgcccgtac
     6061 ccttgtccac ggcgccgcgc agcgcgtcgc gcagctgctg tgcggcggcc aggcccagcg
     6121 cgcgctcggg cgcagcgctc tggaaggctt cgagcagctt gccgtgcttg tcctcgatgc
     6181 gggtgatcac cgtgggcgcc cggtagagcc cgccgctggc gatggtgccg taggcggcca
     6241 ccatctcctt caaggtgacc gcgctggtgc ccagagccag cgcgggcacg gcctccagcc
     6301 tggattcgcg cacgcccatg gcgcgtgcca gctgcgccac ctgctgcggc cccacctgct
     6361 gcatcagctg ggcggtgatg gtgttcttgg aatacgccag gccgtcgcgc agctgcatgg
     6421 gctcgttgct gggcggcgtg cgcccgtccg tggggcgcca gacctgcttg ccgcccagtg
     6481 gtatctccac ggcctgatcc atcagctggt cggtgggcag catgcccttg gcgaaggccg
     6541 cgccgtagac gaagggcttg aaggtggagc cgggctggcg ccgggcggcc tgcacatggt
     6601 cgaacgggtc ctgctcgaag tcgcggctgc ccacccaggc cagcaccgcc ccgctcctcg
     6661 gatcgagcgc caggaagccg gcctgcacgc gcgtcttggc ggcgcgcagc cggcgcaaaa
     6721 aatcgccatc ggccagcagt tgctgcagcg cctcctcggg cgtggcgccg gccgccacgg
     6781 ccttgtcgta ctggggcgac tcgcgcacca gctgctgcac cagcgggctc ctggcgctcc
     6841 aggcggcgcg tggcgcccag gcggcatcgg ccacgccctg cagctgccgg ccctggcgct
     6901 gcacggcctg gtttgccatt tcctgcaggc ggccgtcgat ggtggtgcgt atcaccaggc
     6961 cgtcgctgta caggctgtag ccattgcgat cggcccagtc gatgagctgg cggcgcagct
     7021 gctgcgccag gtgcggggcc aggccggaca gctcgctctg gcgctcgaag cgtatgcgca
     7081 gcgggcgctt ggcgagcttg tcgtactcgg cgctcgcgag cttgccgcgc ttcttcatct
     7141 gcgccagcac ggtgttgcgc cgctcttgcg cgcgctccgg gttgagcacc gggttgtagt
     7201 aggccgtgcc cttgagcatg ccgaccagcg tggcgctctc cagcaccgtc agcctgtcgg
     7261 ccgatttgtc gaaataggta cgcgccgcca tctctatgcc ataggcgttg tagagaaagg
     7321 gcacggtgtt caggtaggtc tccaggatct cgtccttgga gtacaaccac tcgatcttca
     7381 gcgccgtgat ggcctccttg atcttgcgtg tgagcgtggg ggcgcggcca atgtcctcag
     7441 ggtacaggtt gcgcgccagc tgctgggtga tggtggagcc gccctggcgc tcgcccccaa
     7501 aggtgcgcac catggccgat gccgtgcgcc gccagtccag gccaaagtgc tcataaaagc
     7561 ggtggtcctc ggtggcgatc agcgcatcca ccaccggcct ggcgatggtg gaaagcggca
     7621 cccattcgcg gttcacccag cggtattcgg ccagcaggcg cccgtcgctg gagaccagct
     7681 gcgccggctg atcgctcttg gccttgcgga tgtcgctgat ggcgggcgtg aacgggatca
     7741 gcgccagcac gtacagaaca accgccagcg ccagcaccgc cagcagccac agcaggccat
     7801 gggcggacag ccaggagtcg ggccggcgca ccagcgccgc gcacagcccg gccgtgcgcg
     7861 cacgcaagga atcgaaaaac accataaatg ttgataaaaa gtgcctgtag cgcttatgga
     7921 taaagcgcta acagctataa atttgaaagc gtctgcgcgg gccgctgaaa ggccggcgat
     7981 ggtgcggcgc ccggcgccgc ggtgacggca ggcgtgcttt ataccgccaa tcgtttgaaa
     8041 tcaacggttt acaggttttt ccaaacgttc agcccatgaa ctgacgcagc tgcggcaggc
     8101 tggcctgggc agcgcggcgg ccttcgtcga tggcctcctg ggcgcggtga aagtccagct
     8161 ggcccaggtg cgacaggcgc ggcgctatca ccacctcggg cgggtcgccg gccatgcggc
     8221 tgcgcgtgat gcgcatctgc atgatgttca cgctggtcat catcacgtcc agcatcgacg
     8281 gctgcttggg tgccggcgcc ttgccattgc tgccggccca gaacttcagg cgccgcatcc
     8341 agtcgccgtt gtgcgcgggt tcgggcgcgg gcagggcctc ggggccgtcg cccggctggc
     8401 tctcgcgcac cacggccagg gggtgcatat ggcgctgcac gatgtcggcg ttcaagtcca
     8461 cgccgatgac gatgtcggcc cccatggcac gcgccagcga ggtgggcacg ggattcacca
     8521 ggccgccatc gaccagcagc cgcccgtcgg catgcaccac cggcgtgaac agaccgggca
     8581 gtgcaatcga tgcgcgcacg gcgtcggcga tcgagccgtc gcgcagccag acctcggcgc
     8641 ccgagtgcag atcggtcgcc acacaggcga agggcagcgg agacgcatcg atgtcgaagt
     8701 cggcaaaatg ccggcgccag aaggcgatca gcttttcgcc cttgagcatg ccgcccgcca
     8761 gattgaagtc catgaagccg aacacctcgc gcagccccag ggtctgcacc cattcggcaa
     8821 agcgctccag ctcgcccgcc gcataggccg cgcccaccag ggcgccgatc gacgagccgc
     8881 agaccatgtc aaggtgcacg ccctcgtcgc gcaacacctg cagcacgccg atatgcgccc
     8941 agccgcgcgc cgagccgctg cccagcgcca ggcctacctt gggttcgcgc cgtgccaggg
     9001 ccatggctac agcgccccgc gcagcagcgc gtgcaggccg tcctcgtcca gcaccgggat
     9061 gcccaactcc tcggccctgg cgagcttgct gcccgcctcg gcaccggcga cgacgtagtg
     9121 cgtcttcttg ctgaccgagc ctgccacctt ggcgccggcg gcctccagca aatccttggc
     9181 cgcgtcgcga ctcaaggtcg gcagagtgcc ggtcagcacc acggtcttgc cggccagggg
     9241 caaagcggtg tgctcggcgg gtgcaccctc ctcccaggtc acgccatgcg cgcgcaacgc
     9301 agcgaccacc tcgcggttgt gcggctgggc gaagaaggtg tggatggaat cggccaccac
     9361 gggccccacg tcgttcacct gcaagagctg ctccacccca gcgttcatga tggcgtcgag
     9421 gctgccgaaa tgacgtgcca gatccttggc cgtggcctcg cccacatggc gcacccccag
     9481 gccgaacaga aaccgcggta gcgtggtgcg ctttgatttt tgcagcgcat ccagcaggtt
     9541 ctgcgccgac ttctcggcca tgcgctccag gggcgccagc gtgggcagat ccaggcggta
     9601 caggtctgcc aggctgcgca ccagctcacc atcgaccagc tgatcgacca gcttttcgcc
     9661 cagcccctcg atgtccaggg cgcggcgctg cgcgtagtgc agcagcgcct gcttgcgctg
     9721 ggccgggcaa aacagcccgc ccgagcagcg gtggttcatc tcgccggcct cgcgcaccac
     9781 ggcactgccg cacaccgggc attggcgcgg catgcggaag ttgggcacgt aggccgggcg
     9841 ctggccggcg accacgccca ccacctctgg aatcacgtcg cccgcgcggc gcacgatgac
     9901 ctggtcgccc acacgcacgc ccttcttgcg gatctcgaac aggttgtgca gcgtggcatt
     9961 ggtgacggtg acgccgccca cgaacaccgg cgccagccgc gccacgggtg tcagcttgcc
    10021 ggtgcggccg acctgcacgt ctatgccttc gcagcgcgtc agcatctcct gcgccgggta
    10081 cttgtgcgcc acggcccagc gcggctcgcg cgtcttgaag cccatctgcc tttgcagcgc
    10141 caggctgttg accttgtaga ccacgccgtc gatgtcgtag gggagcgcat cgcgctcggc
    10201 gcccaggcgg cggtggaatg ctattagttc agaagcgcct agcgcaatct gaacctgggg
    10261 tgcaaccgga aacccccagg ttttgagttg ctgcagcagc gcgtaatgcg tgccaaagtc
    10321 gggcccgccc tgctccgccg gcgtgacctc gcccaggccg taggcaaaga aggacagcgg
    10381 gcgctgggcg gttatccctg agtcgagctg gcgcaccgcg ccggcggcgg cgttgcgcgg
    10441 gttgacgaag gtcttctcgc cccgctcgcg ctggcgttcg ttcaacgcgc cgaagtcggc
    10501 gcggcgcata taaacctcgc cgcgcacctc cagcaccggc ggcacgtcgg ccggaagcgt
    10561 cagcgggatc tgccggatgg tgcggatgtt gtgcgtcacg tcctcgccca cctcgccgtc
    10621 gccgcgcgtg gcggcctgcg tgaggcggcc gtgctcgtag cgcaggctca tggccaggcc
    10681 gtcgaacttg ggctcggcca cgtattccat gggcggggcc gatgcatcga ggcccagctc
    10741 gcggcgtacg cgggcgtcga aggcctcggc gccgctggcc tcggtatccg tctcggtctg
    10801 gatgctgagc atgggcacgc tgtggcgcac gctcgccagc ccatccatca ccgcgcctat
    10861 cacgcgctgc gtgggcgagt cgggcgtcac caggcctggg taggcgccct ccagcgcctg
    10921 cagctgctgg aacgcgcggt cgtactcggc gtcgggcacg ctgggtgcgt cctgcacgta
    10981 gtactcgtgc gcccagcggt tgagctgcgc ccgcagattc tcaatttttg tagctaccaa
    11041 cgctggcgca gcgggcgctg gagccgaaaa aagatccaaa ctctcggtca tcttgatttt
    11101 ccacggcgct gcgcgtcacc cgcggcgcat gcaactggcg caacccaggc cggggccacc
    11161 gcgcaagggc cgccccgccg cgctggtggc gtccccctgg ggggaaggcg ccgaaggcga
    11221 ctcagggcgg ccatgttgtt agctgaacag ccgccgcgcc agcaccgagc cggccgacag
    11281 ctcatggctg tccagctggt cgtagagctg ctccaggtcg gccgcgatgg gttccatggt
    11341 catggcaggc agcggtgtgc cgttctggtc gcagagcacg ccgtccatgg cctcggcaag
    11401 ctgggcggcc acctcgcgca ggcgggcgaa gggctgttcg ctgcggtgta cctgggcaat
    11461 gtccaggctg agcatgatgt cgcgcacggc ggactgctcg gggtcgtcgg acatggcggc
    11521 ctggctgtcg taacccaggg tcagcatggg tggaaggccg acgccgctgg ccgccagcac
    11581 cagacgcccc ggaatcgcac cggccacgaa gccgcaacgc gctgcattct gctgcacata
    11641 gccggggctc caggcggcct ggcgcgcgcg cagcatgaag gacagctggg cgtcatggtc
    11701 gctggcgaac tgatcgagct cgcgcgcccg cgccacctcg tgcagcatgt cgggaaagtc
    11761 gggcgtggcg ttgatggcgt cggcaaaggc ctgggccttc atgacgaact ccgagaactc
    11821 gatctcgttg agcgcgccca cgcggttggc cagctgcacc ccggcctgga actgcacata
    11881 gcgctggccg ggctggggct gctcccactg gttgctggcc agattcaggc cctcgatggc
    11941 aaagggcttg ctgcccgcgc ggcgcgtggg cggcagggcc gccagggcgg cgtcgcccga
    12001 gacctgctgc tcggcgacga tgggggcgat cacgtcaatg agcggatcga gcccgccgcg
    12061 gcgctcaggc tgtggcacgg gcagatccag cgcgtcgcgc gcatccacgg catggccttc
    12121 gaaatggggc tctgcggcgc tggcctgggc ggcatcgacc tgccccagcc cggcaccgtc
    12181 gaacgccggc tcctggcgca cggccggctc ctgccccttg tcgccctcgc gcgggctggc
    12241 acgcttgggc gtgttgcgcc gggtggtcca ggcgttgtag gcaatgacga cgatcagcac
    12301 cagggcgccg atgatgatga ggctgagttg gaagttgctc atggtgtatg aagttaagcc
    12361 tcggccatgg acagcgcgga ctgcatgtcc acggccacga tgcgcgagac gccctgctcc
    12421 tgcatggtca cgccgatcaa ctgttcggcc atctccatgg cgatcttgtt gtggctgatg
    12481 aacaaaaact gcgtgccgcg cgacatggcg gagaccagct tggcatagcg ctcggtgttg
    12541 gcgtcgtcca gcggcgcgtc cacctcgtca agcaggcaga acggcgcggg gttgagctgg
    12601 aagatggcga acaccagcgc aatcgcggtg agcgccttct cgccgcccga cagcaggtgg
    12661 atggtctggt tcttcttgcc cggcggctgg gccatgacct gcacgcccga gtcgaggatc
    12721 tcgtcgccgg tcatgatgag gcgcgcctgg ccgccgccga acagctcggg gaacatgcgg
    12781 ccaaagtggc cgttgacggc gtcgaaggtg ccctgcagca ggctgcgcgt ctcgccgtcg
    12841 atcttcttga tcgcatcctc cagcgtggtc atggccaatg tcaggtcttc ggtctgcgcg
    12901 tccagaaagc ccttgcgctc gcgcgccagg gtcagctcct ccagcgcggc caggttgacc
    12961 gcgcccaggg ccgtgatctc gcgctgcagg cggtcgatct cgccctgcag gccggcggcg
    13021 cgcacgccgc cttcctcgat ggacagcgcc acggcctgca ggtcggcctg ggcatcctgc
    13081 agcaggctgc tgtactgctc cagccccagg cgcgcggcct gctccttgag ctggaattcg
    13141 gtgatgcgcg cgcgcagcgg gtcgagctgg cgctcgtgct gcatgcgccg ctcgtcgctg
    13201 gcacgcagcc gggccgtgag ctcgtcgtac tggctgcgct gggcgcccag cagtttttcg
    13261 cgctccagtt tcaggtccag cgcctgctgc agcccgccct gggccgccgc gtcggacagg
    13321 cgtgccagct cgtcctgcgc gcgctggcgc tcgtcctgca gcgtgcgcgc ctgcgtggcg
    13381 gccgtgtcca tggtgcgggc cagctcggcg cggcgcgcct gcaggctgcg ctgcgagaac
    13441 tgcgcctcct gcacgcgccg ctccagctgg cgctgctgct cgcggcattc gttcaatcgg
    13501 cgctcggcct cgatcacgcg gtcgcccagc tgcgcatggc gctcctggct gtcggccagc
    13561 tgcatgtcca gctcctcgaa gcgcgcctcg gactgcacgc ggcgctcctg caggtcggac
    13621 agctgtgcct cgacctctgc caggtcggcg ctgatctgcg cgccgcgcgc gcgcgcctgc
    13681 tcggccagct gggtcagctg cagcgtctgc acctgcagct cgtgcgcgcg gccctgggcc
    13741 tcgctggcct cgcggcgcgc ggccaccagg cgctgggatg cgtcggcata ggcggcctcg
    13801 gcccgcacca gggcggtgcg cgcctcttcg gcaatcagct gctgggcgcg cagctctttc
    13861 tccaggtgct cgatttcctg ggcgcgcgcc agcaggccgg actgttcgga gtcctgggca
    13921 aagaagctca ggctgtgcgc cgctacagca tggcccgcgg ccacatagat ggcctggccc
    13981 ggctgcagct gcgcgcggcg gtccagcgcc tcgtccaggg tctgcgccgt aaagcagcca
    14041 tgcagccagt ccaccagcac ggcacgcagg ccgccgttgt caacgcgcag caggtcggaa
    14101 aggcgcggct gcgtggcggt gggctcgggt tgcggggtgg ctggggcgct gtagaaggcc
    14161 aggcgcgcgg gcggggcatc agcgccgccg gcacccagaa agccgcgcac catgtccagc
    14221 cgccccacgg gcaaggccgc caggcgctcg cgcagcgcgg cttccagggc gttctcccag
    14281 ccgggctcga tgtggatccg gctccacagg ccttgcaggc catccagccc atgcctggcc
    14341 agccagggct gcagcttgcc atcgaccttc agcttttcct gcagcgcctt cagggcatcg
    14401 aggcgcgccg acagatccgc ctggcgcgcg gcctcatggt tcaccgtctg ctggcgcgtg
    14461 cggcgctcct cgtcgagctg gggcacgctg tcctgcagct cctcctggcg ggctgccgcc
    14521 agctcggcgc cctcctcggc ctcctgcagc tgctggcgca gattggccag gcgcgcctcg
    14581 tcgggcgcgg ccagggcgtt cctgtcgctg cgcaggcgct cgtggcgcgc gtcgagctgg
    14641 cggctttgct catccaggct gcgctgctcg gcggccagca cctggatctg ctgctgtatc
    14701 tgcaccacgg cgctgcgctg ctgttcgctg cgctgctggg cctggcgcag ggcgtcttcc
    14761 aggtcgggca gctgcatggc ctgctcttcg acctgggctg ccagcagctc ggcctgctcc
    14821 tcggcgttct cgcctgcgcc ggccaggtgc tcggcctcgg cctcggcctc ttgcttgcgc
    14881 gccgaccatt gctggatctg ctcggccagc tgcagcaggc gctgctcc
//
LOCUS       DAOMTQ010000069        14752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_654_length_14752_cov_25.858815, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000069 DAOMTQ010000000
VERSION     DAOMTQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14752
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_654_length_14752_cov_25.858815"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1347)
                     /gene="ftsW"
                     /locus_tag="PK518_08390"
     CDS             complement(64..1347)
                     /gene="ftsW"
                     /locus_tag="PK518_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRM48973.1"
                     /translation="MSAAARPNAGPGLWQRLRALAGARPPESADVLPVRVGGTEYRRT
                     TATPAHVLGFDQALCWVVVLLLAFSVVMVYSASIAMPDNPRFGNIAPHHFVLRHAIAI
                     GVGFVGALLAFQVSMNTWERMAPWLFVVSILLLIAVLIPHVGVVVNGARRWLSLGIMN
                     FQPSELAKFAVLIYAADYMVRKMEVKERFFRAVLPMVVAVGVVGALLLAEPDMGAFMV
                     IAVIAMGILFLGGVNARMFFLIAAVLVAAFAMMVMTSPWRRERIFAYLDPFSEAHALG
                     KGYQLSHSLIAIGRGEIFGVGLGGSVEKLHWLPEAHTDFLLAVIGEEFGLVGVLVLIL
                     LFLWLTRRIMQIGRQAIALDRVFSGLVAQGVGVWMGFQAFINMGVNLGALPTKGLTLP
                     LMSFGGSAILMNLVAIAVVLRVDYENKLLMRGGRV"
     gene            complement(1344..3155)
                     /gene="murD"
                     /locus_tag="PK518_08395"
     CDS             complement(1344..3155)
                     /gene="murD"
                     /locus_tag="PK518_08395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRM48974.1"
                     /translation="MNPEHHDPLLPAEASMPEAAPGAAPVAAPAPAAAPALDWQAPAV
                     SAAEQAREFVARIFADVGAADEPEAAAPVAETEPAAAAELPAAAPTTRPGEGLAPLAD
                     QRVLVLGLGASGLAMARWCVRCGAQVIVADTREAPPQLALLQAELPQVEFIAGPLAAS
                     LVEGRALHAVYRSPGLSPADIAPVTVAAGACQISVGGELDLYAMALRELRASHGYAPA
                     VLAVTGTNGKTTVTSLTTLLVQQAGKSVAVAGNIGPTLLDTLGQRLDAADLPEVWVLE
                     LSSFQLDGVQGFEPTAAAVLNISQDHLDWHGSLQAYAAAKARIFGNSALMVLNREDAA
                     VMAMLPPPVRVKLQKPQPRAHVTFGADMPQRPGDFGIEVVSGMAWLVRAHEADETARR
                     GRNAQEAELYVQRLMPADALRIRGRHNACNALAALALAQAAGCPLAPMLYALREYRGE
                     PHRVEPVGMLREVEYFDDSKGTNVGATVAALTGLGADRRLVVILGGDGKGQDFSPLAA
                     PIARYARAVLLIGRDAPQIRAALAGSGVPLLDAATLPEAVQLASQRAHAGDAVLLSPA
                     CASLDMFDNYAHRARVFVDAVHALAEDAGQSLDGGLA"
     gene            complement(3152..4330)
                     /gene="mraY"
                     /locus_tag="PK518_08400"
     CDS             complement(3152..4330)
                     /gene="mraY"
                     /locus_tag="PK518_08400"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRM48975.1"
                     /translation="MLLILSQWLQGLSPDFGFLRVFQYITFRAVMAALTALLIGLAAG
                     PKVIRILTALKIGQPIRGYAMQSHLSKSGTPTMGGVLILLCIAISTLLWFDLSNRFVW
                     IVLIVTLGFGAIGWADDWRKVVNKDPEGMRSREKYLWQSVIGLAAALYLVFCISENSN
                     ARVFELFVAWIKSGFTLDLPPQAGLLVPFFKEVSYPLGVLGFVILTYLVIVGASNAVN
                     LTDGLDGLAIMPVIMVGASLGIFAYVTGNAGFAKYLLFPYIAGSGELLIFCAAMAGAG
                     LAFLWFNAHPAQVFMGDVGALALGGALGTIAVIVRQEIVLAIMGGIFVVEALSVMLQV
                     SWFKFTKRRYGEGRRLLKMAPLHHHFEKSGWKETQVVVRFWIITMLLCLMGLSTLKLR
                     "
     gene            complement(4321..5718)
                     /gene="murF"
                     /locus_tag="PK518_08405"
     CDS             complement(4321..5718)
                     /gene="murF"
                     /locus_tag="PK518_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRM48976.1"
                     /translation="MSHTNHPPMMTLQQAFELLRARIPAARLVGDGATPLARVHTDTR
                     TLQAGDLFVALKGERFDANTFLPQAGAAGAAAAIAHGGLQAAGLPGIEVPDTLAALGA
                     LAAGWRAQFDLPLIAVTGSNGKTTVTQMLAAVLRAYKGDAALATQGNFNNDVGLPLTL
                     LRLAATHQAAVVELGMNHPGEIAYLADITRPSVALVNNAQREHLEFMHTVDAVARENG
                     SVISALAQDGVAVFPAGDEYTPLWRALAGERCCLCFGAQGDVRCETAQWNEGAWAVRI
                     ATPLGAFDTRLHIAGLHNVTNAMAATACAVAAGVPLAAIAAGLAAFEPVKGRSRALAL
                     HCQGRAITVVDDSYNANPDSVRAAIAVLADLPGPRLLVLGDMGEVGNQGPQFHAEAGA
                     HAHQSGIEQLFALGALSVHAAQACGPAARHFEDMASLLAAVQEALPGVGSVLVKGSRF
                     MKMEQVVQAMEGACS"
     gene            complement(5715..7220)
                     /locus_tag="PK518_08410"
     CDS             complement(5715..7220)
                     /locus_tag="PK518_08410"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRM48977.1"
                     /translation="MSRPRPLASVAEAVAWLRARVTGTLQTDSRLIQSGDAFIAWPGA
                     ATDGRAHVADALARGAVACLVEQQGVEAFALAGVHVAALPGLKAATGLIAAEWFGRPS
                     ERLQVLAVTGTNGKTSTAWWLADALNQLSTQELPALDGCALVGTLGIGVPPELNSTGM
                     TTPDPVRLQRAFAQFADAGLSACAIEASSIGLAEHRLDGTQMRVALFTNFTQDHLDYH
                     HDMAAYWQAKRVLFDWPGLRAAVINIDDAQGAALHAELQGRALDLWSVSIQGPARLAA
                     QDIVHRGAGLAFTVVEGGQALPLQTQLIGLYNVSNVLGVIACMRALGVPLAQALAACA
                     RLAPVPGRMQQLAFAGQPLVAVDYAHTPDALHQALTALRPMAAQRGGRLWCVFGCGGN
                     RDATKRPLMGAVAQREADRVIVTSDNPRGEEPRAIIHQILQGMIASTHVGAEVDRAAA
                     IAQAVAEAGANDVVLIAGKGHEDYQETAGQRQPFSDMAQARDALARRGAVA"
     gene            complement(7217..8965)
                     /locus_tag="PK518_08415"
     CDS             complement(7217..8965)
                     /locus_tag="PK518_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRM48978.1"
                     /translation="MSRRSVNYTSSPLLASKTPLWRSQFIVALVALGFLALIGRAAYV
                     QVFGNAFFQRQGEVRFARTLELPANRGRILDRNGLILASSVPAASIWAIPEDVETDNP
                     EVKPKLKQLARLMGMPLPALMAKLADEDKTFVWIKRQLDWDVGQQIQALGIKGIYQRK
                     EYKRQYPEGEAAAHVVGFTNVEDHGQEGMELAFDKELAGKPGSRRVIKDRLGRVVEGV
                     GAEVPPVDGRDMQLSIDSKVQFFAYQKLRDTVAEHKAKAGSVVVLDAHTGEVLALANY
                     PSYDPGSRGRLTGEQLRNRAITDTFEPGSTMKPITIGLALNSGRVRPETVIDTSPGRL
                     TITGSTISDTHSYGALTVEGVVQKSSNVGTTKIAMQMPAREMWETFSAVGFGQKPQIA
                     FPGAVSGRLRPYKSWRPIEQATMSYGYGLSASLFQMARSYTVFANGGKVIPATMLKTD
                     EPAVGVPVFSERTANQVRKMLQMAAGPGGTGQKAQTVGYSVGGKSGTARKQVGKSYAT
                     GKYRAWFTGLAPVDKPRIIVAVMIDEPSNGVIYGGAVAAPVFSEVVQQTLRMMSVAPD
                     MAVQPLIVTNAVEESL"
     gene            complement(8962..9279)
                     /gene="ftsL"
                     /locus_tag="PK518_08420"
     CDS             complement(8962..9279)
                     /gene="ftsL"
                     /locus_tag="PK518_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRM48979.1"
                     /translation="MTRISLVLLLAVMASAMYLVHTQYQSRRLYTELDRAVAESRRLE
                     TEHQRLQVEKRAQATPLRVERLARDKLHMRTATPAITHYVTADGKPVAAPLAAPAAAG
                     GRR"
     gene            complement(9276..10208)
                     /gene="rsmH"
                     /locus_tag="PK518_08425"
     CDS             complement(9276..10208)
                     /gene="rsmH"
                     /locus_tag="PK518_08425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRM48980.1"
                     /translation="MNQPLQHTTVLLDEAVNALLGGGGDAAPAGCFVDGTFGRGGHSR
                     LILQRLGAAGRLLAFDKDPAAIQEAARIEDARFAIRHQGFSQLGELPAASVAGVLLDL
                     GVSSPQIDDPGRGFSFRFDGPLDMRMDTTRGQSVADWLADAEAAQIAEVIRDYGEERF
                     AGPIAKAIVAWRTQRGPIGSTAELADIVAGAVKTREPGQNPATRTFQALRIFINAELE
                     ELQRALGASLRVLAPGGRLVVISFHSLEDRIVKQFIAQHSKEVFDRRAPFAAPQPMRL
                     KAVARIKPGAAEVAANPRARSAVMRVAERTEVPG"
     gene            complement(10218..10646)
                     /gene="mraZ"
                     /locus_tag="PK518_08430"
     CDS             complement(10218..10646)
                     /gene="mraZ"
                     /locus_tag="PK518_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRM48981.1"
                     /translation="MFQGASSLSLDAKGRLSVPTRHRDALTEQAGGQLTITKHPDGCL
                     MVFPRPEWEKFRERIAQLPMSAQWWKRIFLGNAMDVEMDGTGRVLVSPELREAAGLTK
                     EAVLLGMGSHFELWDKATHDAKEAAAMQAGMPDVLKDFSF"
     gene            complement(10909..12225)
                     /gene="hslU"
                     /locus_tag="PK518_08435"
     CDS             complement(10909..12225)
                     /gene="hslU"
                     /locus_tag="PK518_08435"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRM48982.1"
                     /translation="MSSMTPQEIVSELDRHIVGQQGAKRAVAIALRNRWRRQQVDTSL
                     RQEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGKDVDSIVRD
                     LVEVAVKQTREQDMKKVRVRAEDAAEERILDVLIPPARTGEAAPDSTARQVFRKKLRQ
                     GELDDKEIEIDLADARPQLEIMGPQGMEEMAEQLRGMFSQMGQERRKTRKLKIAEALK
                     LLTDEEAAKLVNEEEVKAHALHNAEQNGIVFIDEIDKVASRQESGGAEVSRQGVQRDL
                     LPLVEGTTVSTKYGMVKTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELTSLSVQD
                     FEAILTQTHASLVKQYQALLATEGVALEFTPEGITRLAHIAYDVNERTENIGARRLST
                     VMERLLDEVSFDAARLSGQTVVVDADYVNARLQALSQDEDLSRYIL"
     gene            complement(12296..12841)
                     /gene="hslV"
                     /locus_tag="PK518_08440"
     CDS             complement(12296..12841)
                     /gene="hslV"
                     /locus_tag="PK518_08440"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRM48983.1"
                     /translation="MEQYHGTTILSVRRQTANGIQVALGGDGQVTLGNIVVKGTARKV
                     RKLYQGRVLAGFAGATADAFTLFERFEAKLEKHQGQLTRAAIELTKDWRTDRVLRRLE
                     AMLAVADHSASLIITGNGDVLEPEEGIVAIGSGGAYAHSAAKALLHNTDLPAVDIVKK
                     SLAIAGELCIYTNMHHTIETL"
     gene            complement(12908..14620)
                     /locus_tag="PK518_08445"
     CDS             complement(12908..14620)
                     /locus_tag="PK518_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRM48984.1"
                     /translation="MSKDDTPAPGGLLSKMVKFVRNPTVNWSDLDSLETDRDSQYSKQ
                     MLKEMIERKRRNDFVRKREFDQLRKLRQREAQGGRRLDDALARASFFSNSMTSPDERA
                     GTLKKIDEIEAQMSQQWWKGKEQGAPAPQTASQQGAAPESQRMVVAPPIVPPRPAVVL
                     AAQAALGDQVHAFAPTAPMSMPASLGQGALQVPPAADAAMAAAVPSLVADAEPFEHDP
                     DLEEAAIRFASGDFEGAEAGLREILTQPPQDGARPDEVWLALFDLYRATGQQDPFDML
                     AIEFAARFGRSAPLWFSLPEQLGMQAAVDPATPAAAQFEHGWSAPTLLTQQSVAALQA
                     SLARAAPPWTLSWTRLTEIEPPAIAALAQQFEDWAGRAVQIRFAGVQALQGLLQANTP
                     SGDRSREPAWWRLRMAALRLMGQHDEFELVALDYCVTYEVSPPSWLSPHCAFSGDDGA
                     APAALEGAPDSDLFMSGFALSQPARTDDGPVAALQGQIDGDATPLLQPLEAMAPAGAP
                     LVVSCERLIRIDFAAVGSVLNWAAEQQAQGRAVHFTQLHRLVAVLFNVIGINEHARVV
                     LRKN"
BASE COUNT         2363 a         5103 c         4993 g         2293 t
ORIGIN      
        1 ccaggccggg gaagatatgg ccgccggtgc cgccggccat gatgagggcg gtcttggggc
       61 tgctcatacc ctgccacctc gcatcaagag tttgttctca tagtcaacac gcaaaacaac
      121 ggcaatggcc accaggttca tcagaatggc cgatccgcca aagctcatca aaggcagcgt
      181 cagccccttg gtgggcagcg cgcccaggtt cacgcccatg ttgatgaagg cctgaaagcc
      241 catccacacg cccacgccct gggccaccag gcccgagaac acgcggtcca gcgcgatggc
      301 ctggcggccg atctgcatga tgcggcgcgt gagccacagg aacagcagga tgagcaccag
      361 cacgcccacc aggccgaact cctcgccgat caccgccagc aggaagtcgg tgtgcgcctc
      421 gggcagccag tgcagctttt ccacgctgcc ccccaggccc acgccgaaga tctcgccgcg
      481 cccgatggcg atcagcgaat gtgacagctg gtagcccttg cccagcgcat gcgcctcgct
      541 gaatggatcc aggtaggcaa agatgcgctc gcgccgccag gggctggtca tgaccatcat
      601 ggcaaacgcc gccaccagca cggcggcgat caggaagaac atgcgcgcgt tcaccccgcc
      661 cagaaacaaa atgcccatgg cgatcacggc gatcaccatg aaggcgccca tgtcgggctc
      721 ggccagcagc agcgcgccca ccacgcccac ggccacgacc atgggcagca cggcgcgaaa
      781 gaaccgctcc ttgacctcca tcttgcgcac catgtagtcg gcggcgtaga tcagcacggc
      841 gaacttggcg agctccgacg gctggaagtt catgatgccc agggacagcc agcgccgcgc
      901 gccattgacc accacgccca catgcgggat cagcaccgcg atcagcagca ggatggagac
      961 tacgaagagc cagggcgcca tgcgctccca ggtgttcatg gacacctgga aggccagcag
     1021 cgcgccgaca aagcccacac cgatggcgat cgcatggcgc agcacgaaat ggtggggcgc
     1081 gatgttgcca aagcgtgggt tgtccggcat ggcaatcgag gccgaataca ccatcaccac
     1141 gctgaaggcc agcagcagca ccaccaccca gcacagcgcc tggtcaaaac ccagcacatg
     1201 cgccggcgtg gccgtggtgc ggcggtattc ggtgccgccc acgcgcaccg gcagcacgtc
     1261 ggcggactct ggcgggcgcg cacccgccag cgcgcgcagg cgctgccaca ggccggggcc
     1321 ggcattggga cgtgcggcgg cgctcatgcc agacctccat ccaggctctg gcccgcatct
     1381 tcggccaggg cgtgcaccgc atccacgaac acgcgcgcgc ggtgggcgta gttgtcgaac
     1441 atgtccaggc tggcgcaggc cggcgacagc agcacggcgt cgcccgcatg ggcgcgctgg
     1501 ctggccagct gcacggcctc gggcagggtg gcggcatcca gcagcggcac accgctgcca
     1561 gccaaagcgg cgcggatctg cggcgcgtca cgccctatca gcagcaccgc acgcgcatag
     1621 cgcgcaatcg gcgcggccag cggcgagaag tcctggccct tgccgtcgcc gcccaggatg
     1681 accaccaggc gcctgtccgc gcccaggccg gtgagggcgg ccacggtggc gccgacgttg
     1741 gtgcccttgc tgtcgtcgaa gtactccacc tcgcgcagca tgccgacagg ctcgacgcgg
     1801 tgcggctcgc cgcggtattc gcgcagggcg tagagcatgg gcgccagcgg gcagcctgcg
     1861 gcctgggcca gggccagcgc ggccagggcg ttgcaggcgt tgtggcggcc gcgtatgcgc
     1921 agggcgtcgg cgggcatcag gcgctggacg tagagctcgg cctcctgggc attgcggccg
     1981 cgcctggcgg tctcgtcagc ctcgtgcgcg cgcaccagcc aggccatgcc gctgaccacc
     2041 tcgatgccga agtcgccggg gcgctgcggc atgtcggcgc caaaggtcac atgggcgcgc
     2101 ggctggggct tttgcagctt gacgcgcacc ggcggcggca gcatggccat gacggcggcg
     2161 tcctcgcggt tgagcaccat cagcgcgctg ttgccaaaga tgcgcgcctt ggccgcggcg
     2221 taggcctgca ggctgccatg ccagtccagg tggtcctggc tgatgttgag cacggcggcg
     2281 gccgtgggct cgaaaccctg cacgccatcc agttgaaagc tcgacagctc cagcacccag
     2341 acctcaggca gatcggccgc gtccaggcgc tggcccaggg tgtcgagcag cgtcgggccg
     2401 atattgccgg ccacggccac gctttttccc gcctgctgca ccagcagggt ggtcagcgat
     2461 gtcaccgtcg tcttgccgtt ggtgccggtg acggcgagca cggccggcgc atagccatgg
     2521 ctggcgcgca gttcgcgcag cgccatggca tacaggtcta attcgcctcc aacgcttatc
     2581 tggcaagcgc cagcagctac tgtaacggga gcaatatcag ccggactcag gcccggcgag
     2641 cggtacacgg catgcaacgc gcggccctcg accaggctag cggccaaggg cccggcgatg
     2701 aactccacct ggggcagctc ggcctgcagc agggcgagct gcggcggggc ttcgcgcgtg
     2761 tcggccacga tgacctgggc accgcagcgc acacaccagc gcgccatggc caggccggag
     2821 gcgcccaggc ccagcaccag cacgcgctgg tcggccagcg gcgccaggcc ctcgccgggg
     2881 cgggtggtgg gtgcggcggc aggtagttct gcagccgcgg cgggctcggt ctcggccacg
     2941 ggcgcggcgg cctcgggttc gtcggcggcg cccacgtcgg caaagatgcg cgcgacgaac
     3001 tcccgggcct gctcggcggc agaaacggcc ggcgcctgcc agtcgagagc gggcgcggcc
     3061 gccggggccg gtgcagcaac cggcgcggcg cctggcgcgg cttcgggcat ggaggcctcg
     3121 gccggcagca ggggatcgtg gtgctctggg ttcatcgcag tttcagcgtg gacaggccca
     3181 tcaggcacag cagcatggtg atgatccaga agcgcacgac gacctgcgtc tccttccagc
     3241 cgctcttttc gaaatggtgg tgcagcggcg ccatcttcag caggcgccgg ccctcgccgt
     3301 agcggcgctt ggtgaacttg aaccagctca cctgcagcat caccgacagc gcctcgacga
     3361 cgaagatgcc gcccatgatg gccagcacga tctcctggcg cacgatgacg gcgatggtgc
     3421 ccagggcgcc acccagcgcc agcgcgccca cgtcgcccat gaacacctgg gccggatggg
     3481 cgttgaacca cagaaaggcc aggccggcac cggccatggc ggcgcagaag atcagcagtt
     3541 cgcccgagcc ggcgatgtag ggaaacagca ggtacttggc aaagccggca ttgcccgtca
     3601 cataggcaaa gatgcccagc gacgcgccca ccatgatcac cggcatgatg gccaggccgt
     3661 ccaggccgtc ggtcaggttc acggcgttgc tcgcgcccac gatcaccagg taggtcagga
     3721 tcacgaagcc cagcacgccc agcgggtagc tgacctcctt gaagaagggc accagcaggc
     3781 cggcctgcgg cggcagatcg agcgtaaagc ccgacttgat ccaggcgaca aacagctcga
     3841 acacgcgtgc gttcgagttc tcggagatgc agaacaccag gtacagcgct gcggccaggc
     3901 cgatcaccga ttgccagagg tatttctcgc gcgagcgcat gccctcggga tccttgttca
     3961 ccaccttgcg ccagtcgtcg gcccagccga tggcgccaaa gcccagcgtg acgatgagca
     4021 cgatccagac aaagcggttg gacaggtcga accacagcag cgtggagatg gcgatgcaca
     4081 gcaggatcag caccccgccc atggtgggcg tgccgctctt ggacaggtgc gactgcatgg
     4141 cgtagccgcg gataggctgg ccgatcttca gcgccgtgag tatgcgtatc accttggggc
     4201 cggccgccag gccgatgagc agcgccgtca gcgccgccat caccgcacgg aaggtgatgt
     4261 actggaacac gcgcagaaag ccgaagtccg gcgacaggcc ttgcagccat tgcgacagga
     4321 tcaggagcat gcgccctcca tggcctgcac cacctgttcc atcttcatga agcgcgatcc
     4381 cttcaccagc acgctgccca cgccgggcag ggcctcctgc accgctgcca gcagcgatgc
     4441 catgtcctcg aaatgccgcg ccgccggccc gcaggcctgg gcggcatgga cgctcaaggc
     4501 gcccaaggca aacagctgct ctatgccgct ttggtgcgca tgcgcgccgg cctcggcatg
     4561 gaactgcggc ccctggttgc ccacctcgcc catgtcgccc agcaccagca ggcgcgggcc
     4621 gggcagatcg gccagcacgg ctatggcggc gcgcaccgag tcggggttgg cgttgtagct
     4681 gtcgtccacc acggtaatgg cccggccctg gcaatgcagg gccagcgcgc gcgagcggcc
     4741 cttgaccggc tcgaacgccg ccaggccggc ggcaatggcg gccagcggca cgcccgcggc
     4801 cacggcgcag gcagtggccg ccatggcgtt ggtgacgttg tgcaagccgg cgatatgcag
     4861 gcgcgtgtcg aatgcgccca ggggcgtggc aatgcgcacg gcccaggcgc cctcgttcca
     4921 ctgcgcggtc tcgcagcgca catcgccctg cgcgccaaag cacaggcagc agcgctcacc
     4981 cgccagcgcg cgccacagcg gcgtgtactc gtcgccggcc ggaaacaccg ccacaccgtc
     5041 ctgcgccagg gcgctgatga cgctgccgtt ctcgcgcgcc accgcgtcca ccgtgtgcat
     5101 gaattccaga tgctcgcgct gggcgttgtt caccagggcc acgctggggc gggtgatgtc
     5161 ggccaggtag gcgatctcgc ccgggtggtt catgcccagc tcgaccaccg ccgcctgatg
     5221 cgtggcggcg aggcgcagca gcgtcagcgg caggcccacg tcattgttga agttgccctg
     5281 cgtggccagg gccgcatcgc ccttgtaggc gcgcagcacg gcggccagca tctgcgtgac
     5341 ggtggtcttg ccgttgctgc cggtcacggc tatcagcggc agatcaaact gagcgcgcca
     5401 gccggccgcc agcgcgccca gggccgccag ggtgtcgggc acctcaatgc cgggcaggcc
     5461 cgccgcctgc aacccgccat gggcaatggc ggcggcggcg ccggccgcgc cggcctgggg
     5521 cagaaaggtg ttggcgtcga aacgctcgcc cttcagggcc acgaacaggt cgccggcctg
     5581 cagcgtgcgg gtgtcggtgt gcacgcgcgc cagcggcgtg gcgccgtcgc ccaccaggcg
     5641 cgcggccggg atgcgggcgc gcagcagctc gaaggcctgc tgcagcgtca tcatgggggg
     5701 gtggttggtg tggctcatgc cacggctcct cgacgcgcca gcgcgtcacg cgcctgcgcc
     5761 atgtcggaga acggctggcg ctggccggcc gtctcctggt aatcctcgtg acccttgccg
     5821 gcgatcagca ccacgtcgtt ggcgccagcc tcggcgacgg cctgggctat ggctgcggca
     5881 cggtcaactt ctgcgcccac atgggtggag gcaatcatgc cctgcaggat ctggtgaatg
     5941 atggcgcgcg gctcctcgcc acgcgggttg tcgctggtga cgatgacgcg gtcggcctcg
     6001 cgctgcgcca cggcgcccat cagcgggcgc ttggtggcgt cgcggttgcc gccacagccg
     6061 aacacgcacc acagccggcc accgcgctgc gccgccatgg gacgcagcgc cgtcagcgcc
     6121 tggtgcagcg cgtccggcgt gtgggcatag tccacggcaa ccaggggttg gccggcaaag
     6181 gccagctgct gcatgcgccc cggcacgggt gccaggcgcg cgcaggcggc cagggcctgc
     6241 gccaaaggca cgcccagcgc gcgcatgcag gcgatcacgc ccagcacgtt cgacacgttg
     6301 tacaggccga tcagctgggt ctgcagcggc agcgcctgcc cgccctcgac cacggtgaag
     6361 gccaggcccg cgccgcgatg cacgatgtcc tgtgcggcca gccgcgccgg accctggatg
     6421 gacacgctcc acaggtccag cgcgcggccc tgcagctcgg catgcagggc ggcgccctgg
     6481 gcgtcgtcga tgttgatgac ggcagcgcgc agccccggcc agtcgaacag cacgcgcttg
     6541 gcctgccagt aggcggccat gtcgtggtgg tagtccaggt ggtcctgggt gaagttggtg
     6601 aacagcgcca cgcgcatctg cgttccatcc agccggtgtt cggccagacc tatggacgag
     6661 gcttcgatgg cgcaggccga gagccccgca tcggcaaact gcgcaaacgc acgctgcagg
     6721 cgcacggggt cgggcgtggt catgccggtg ctgttcagct ccggcggcac accaataccc
     6781 aaagtaccta ccaaggcgca cccatcaagc gcaggcagct cttgtgttga tagttgattg
     6841 agcgcatctg ccagccacca ggcggtgctg gtcttgccat tggtgccggt cacggccagc
     6901 acctgcaggc gctcgctggg ccggccaaac cattcggcgg cgatcaggcc ggtggcggcc
     6961 ttgaggcccg gcagcgccgc gacatgcaca ccggccaggg cgaaggcctc caccccctgc
     7021 tgctccacca ggcaggcgac ggcgccgcgc gccagggcgt cggccacatg ggcgcggccg
     7081 tcggtggcgg cgccgggcca ggcgatgaag gcgtcgccac tctggatcag gcggctgtcg
     7141 gtctgcaagg ttccggtgac gcgcgcgcgc agccaggcaa cagcctcggc tacggatgcc
     7201 agggggcgcg gccggctcac agcgactcct ccacggcgtt ggtgacgatc agcggctgca
     7261 cggccatgtc gggcgccacg ctcatcatgc gcagcgtctg ctgcaccacc tcgctgaaca
     7321 ccggggcggc cacggcaccg ccgtagatca cgccgttgct cggctcgtcg atcatcacgg
     7381 cgacgatgat gcgcggcttg tccaccggcg ccaggccggt gaaccaggcg cggtacttgc
     7441 ccgtggcata gctcttgccc acctgcttgc gcgccgtgcc cgacttgcca cccaccgagt
     7501 aacccacggt ctgcgccttc tggcccgtgc cgccggggcc ggcggccatc tgcagcatct
     7561 tgcgcacctg gttcgccgtg cgctcggaga acaccggcac gcccacggcg ggctcatcgg
     7621 tcttgagcat ggtggccggg atgaccttgc cgccattggc gaacacggtg taggagcgcg
     7681 ccatctggaa caggctggcc gacaggccat agccgtagga catggtggcc tgctcgatgg
     7741 ggcgccagct cttgtagggg cgcagccgcc cgctgaccgc accggggaag gcgatctgcg
     7801 gcttctgccc gaagccgacg gcggagaagg tctcccacat ctcgcgcgcc ggcatctgca
     7861 tggcgatttt tgtcgtaccc acgttgctcg acttctgcac cacgccctcg acggtcaacg
     7921 caccgtagct gtgcgtgtcg ctgatggtgg agccggtaat ggtcagtcgc ccggggctgg
     7981 tgtcgatcac ggtctcgggc cgcacgcggc cgctgttgag cgccaggcct atggtgatgg
     8041 gcttcatggt cgagccgggc tcgaaggtgt cggtgatggc gcggttgcgc agctgctcgc
     8101 cggtcagcct gccgcggctg ccggggtcgt agctggggta gttggccagg gccagcacct
     8161 cgccggtatg ggcgtccagc acgaccacgc tgccggcctt ggccttgtgc tcggccacgg
     8221 tgtcgcgcag cttctggtat gcgaagaact gcaccttgct gtcgatggac agctgcatgt
     8281 cgcgcccatc gacgggcggc acctcggcgc ccacgccctc gaccacacgg cccaggcggt
     8341 ccttgatcac gcggcgcgaa ccgggcttgc cggccagctc cttgtcaaac gccagctcca
     8401 tgccctcctg gccatggtct tccacgttgg tgaagccgac cacatgggcg gccgcctcgc
     8461 cctcggggta ctggcgcttg tattccttgc gctggtagat gcccttgatg cccagggcct
     8521 ggatctgctg gcccacatcc cagtcgagct ggcgcttgat ccagacgaag gtcttgtctt
     8581 catccgccag cttggccatg agtgccggca ggggcatgcc catcaggcgc gccagctgct
     8641 tgagtttggg cttcacctcc gggttgtcgg tctccacgtc ctcgggaatg gcccagatgc
     8701 tggccgccgg cacgctggac gcgaggatca ggccgttgcg gtccagaatg cggccacggt
     8761 tggccggcag ctccagcgtg cgtgcaaagc gcacctcgcc ctggcgctgg aagaaggcgt
     8821 tgccgaacac ctgcacatag gccgcgcgcc cgatcagggc cagaaagccc agggccacca
     8881 gggccacgat gaactggctg cgccacagcg gcgtcttgct ggccagcaag gggctggagg
     8941 tgtagttgac gctgcgccgg ctcatcgcct gccccctgct gccgcgggtg cggccagtgg
     9001 cgcggcaacg ggcttgccgt cggccgtcac gtagtgggtg atggccggcg tggccgtgcg
     9061 catgtgcagc ttgtcgcgcg ccaggcgctc cacgcgcagc ggcgtggcct gggcacgctt
     9121 ttccacctgc aggcgctggt gctcggtttc caggcggcgc gattcggcca ccgcgcggtc
     9181 gagctcggtg tacagccggc gtgactggta ctgggtgtgc accagataca tggcgctggc
     9241 catcaccgcc agcagcagca ccaggctgat acgggtcatc ctggcacctc cgtgcgctcg
     9301 gccacgcgca tcacggcgct gcgcgcacgc gggttggcgg ccacctcggc ggcgcccggc
     9361 ttgatgcggg cgacggcctt caggcgcatg ggctggggcg cggcgaacgg cgcgcggcgg
     9421 tcgaacacct ctttcgaatg ctgggcgatg aactgcttga cgatgcgatc ttccagcgag
     9481 tgaaaactga tcaccaccag gcggccgccg ggtgccagca cgcgcaggct ggcccctagc
     9541 gcgcgctgca gctcctcaag ctcggcgttg atgaaaatcc ggagcgcctg aaatgtgcgc
     9601 gtcgcagggt tctggcccgg ctcgcgggtc ttgaccgcgc cagccacgat gtcggccagc
     9661 tcggcagtgc tgccaatggg gccgcgctgc gtgcgccaag caacaatcgc ctttgcaatg
     9721 gggccagcaa accgttcttc gccgtagtca cgtatcacct ccgcaatctg tgccgcctcg
     9781 gcatctgcca gccaatcggc cacgctctgg ccgcgcgtgg tgtccatgcg catgtccagc
     9841 gggccgtcga atcgaaaact gaaaccgcgc ccggggtcgt cgatctgcgg cgagctcacg
     9901 cccaggtcca gcaacacacc ggccacgctg gccgcaggca gctcgcccag ctgcgaaaag
     9961 ccctggtgcc gaatggcaaa gcgcgcatcc tcgatgcgcg ccgcttcctg gatggctgcc
    10021 gggtccttgt cgaaggccag cagccgcccc gcagcgccca gccgctgcaa tatgagccgc
    10081 gaatgccctc cgcggccaaa ggtgccgtcg acgaagcagc ccgccggcgc ggcgtcaccg
    10141 ccgccgccca gcagggcatt gaccgcctcg tcgagcaaga ccgtggtgtg ttgcaggggc
    10201 tggttcacac gcggccccta gaaagaaaag tccttgagga catcgggcat gccggcctgc
    10261 atggcggcgg cctccttggc gtcgtgcgtc gccttgtccc agagctcgaa gtggctgccc
    10321 atgccaagca gcacggcctc cttggtcagg cccgcggcct cgcgcaactc gggcgagacg
    10381 agcacgcgcc ccgtgccatc catctccacg tccatggcgt tgcccaggaa gatgcgcttc
    10441 caccactgcg ccgacatggg cagttgggcg atgcgctcgc ggaacttctc ccactccggg
    10501 cgcggaaaca ccatcaggca gccgtcgggg tgcttggtga tggtcagctg gccgccggcc
    10561 tgctccgtca gggcgtcacg atgccgggtc ggcacagata gccgcccctt ggcatcgaga
    10621 ctcagcgatg acgccccttg gaacacgaat cgaacccctg cttgatccac ccacctttcg
    10681 gcgccgtttt gggctcgaaa ggcacttttc accacttaat tgcacttttt tgcactgtag
    10741 caggaaaagc acgcccgaca acaggggagt cgtcaaaaat ttgtaatgaa atcaagggct
    10801 tagaacccga tttgcatgca aaaatcaggc aaaacccttt attgattaag cacttagatg
    10861 cgggtatgca agtgctttat gaagcccagg gcgatgtcac cccgaccctc acaggatgta
    10921 gcgcgacagg tcttcgtcct ggctcagcgc ctgcagacgc gcgttcacgt agtcggcgtc
    10981 cactaccacc gtctggcccg acaggcgggc ggcgtcgaag ctgacctcgt cgagcagccg
    11041 ctccatcacc gtggacagcc ggcgtgcgcc gatgttctcg gtgcgctcgt tcacgtcata
    11101 ggcaatatgc gccaggcgcg tgatgccctc gggggtgaat tccagcgcca cgccctcggt
    11161 ggccagcaac gcctggtact gcttgaccag gctggcatgg gtctgcgtca ggatggcctc
    11221 gaagtcctgc accgacagcg aggtgagctc cacgcggatc ggaaagcgcc cctgcagctc
    11281 ggggatcagg tcgctgggct tggccaagtg gaaggcgccc gaggcgatga acaggatatg
    11341 gtcggtcttg accatgccgt acttggtgga caccgtcgtg ccctccacca gcggcagcag
    11401 gtcgcgctgc acgccctggc gcgagacctc ggcgccgccg ctctcctggc gcgaggccac
    11461 cttgtcgatc tcgtcgatga agacgatgcc gttctgctcg gcgttatgca gggcatgggc
    11521 cttgacctct tcctcgttca ccagcttggc agcctcctcg tcggtcagca gcttgagcgc
    11581 ctcggcgatc ttcagcttgc gcgtcttgcg ccgctcctgg cccatctggc tgaacatgcc
    11641 acgcagttgc tcggccatct cttccatgcc ctgcgggccc atgatctcca actgcgggcg
    11701 cgcgtccgcc aggtcgatct ctatctcctt gtcgtccagt tcgccctggc gcagcttctt
    11761 gcggaacacc tggcgcgccg tgctgtcggg tgccgcttcg ccggtgcgtg ccggggggat
    11821 caacacatcg agaatgcgct cctcggccgc gtcttcggcg cgcacgcgca ccttcttcat
    11881 gtcctgctcg cgcgtctgct tcacggccac ctcgaccagg tcgcgcacga tggagtccac
    11941 atccttgccg acatagccca cctcggtgaa cttggtggcc tcgaccttga tgaagggcgc
    12001 atcggccagg cgcgccaggc gacgcgcaat ctcggtcttg ccgacgcccg tggggccgat
    12061 catcaggatg ttcttgggcg tgatctcctg gcgcaggctg gtatcgacct gctggcggcg
    12121 ccagcggttg cgcagggcga tggccacggc acgcttggcg ccctgctggc cgacgatgtg
    12181 gcggtcgagc tcggagacga tttcctgggg ggtcatggag gacattttta gaccatttct
    12241 ggctccagcg ctttacagat aagcgccagc agctattaat acaatagcga aacgcctaca
    12301 gcgtctctat ggtgtggtgc atgttggtat agatgcacag ctcaccggcg atcgcgagcg
    12361 acttcttcac gatgtccacg gccggcagat cggtgttgtg cagcagcgcc ttggcggccg
    12421 aatgcgcgta ggcgccgccc gaaccgatgg cgactatgcc ctcctcgggc tcgagcacgt
    12481 cgccgttgcc ggtgatgatg agcgaggccg aatgatcggc cacggccagc atggcctcca
    12541 ggcgacgcag cacgcggtcg gtgcgccagt ccttggtgag ctcgatggcc gcgcgcgtca
    12601 gctggccctg gtgcttctcc agcttggcct cgaagcgctc aaacagcgtg aaggcgtccg
    12661 ccgtggcgcc ggcaaagccg gccagcacac gcccctggta gagcttgcgc accttgcgcg
    12721 ccgtgccctt gacgacgatg ttgcccaggg tgacctggcc gtcgccgcca agagcaacct
    12781 ggatgccgtt cgccgtctgg cgccggacgc tgagaatggt ggtgccgtgg tactgttcca
    12841 tgggaatgcc atatgagggc gcaggcgccc gtttgcaagc cgcacccacg cccgtggggg
    12901 acaaaaatca gtttttgcgc agcacgacgc gcgcgtgctc gttgatgccg atcacgttga
    12961 acagcacggc caccagccgg tgcagttgcg tgaaatgcac cgcccggccc tgggcctgtt
    13021 gctcggccgc ccagttgagc accgagccca cggcggcaaa gtcgatgcgg atcagccgct
    13081 cgcaggacac gaccagcggc gcacccgcag gcgccatggc ctccagcggc tgcagcagcg
    13141 gcgtggcatc gccatcgatc tggccctgca atgccgccac cgggccatcg tccgtgcgcg
    13201 ccggctggga cagggcaaaa cccgacatga acagatcgct gtccggcgcg ccctccagcg
    13261 ccgcaggcgc ggcgccatcg tcgccgctaa aggcgcaatg cggcgacagc caggacggtg
    13321 gcgagacctc ataggtcacg caatagtcca gggccaccag ctcgaactcg tcatgctggc
    13381 ccatcaggcg cagcgccgcc atgcgcaggc gccaccaggc aggctcgcgg ctgcggtcgc
    13441 ccgatggggt atttgcctgc agcaggccct gcagcgcctg cacgccggca aaccggatct
    13501 gcaccgcgcg cccggcccag tcctcgaact gctgggccag cgcggcgatg gccggcggct
    13561 cgatctcggt caggcgcgtc cagctcaggg tccagggtgg cgcggcgcgc gccagggagg
    13621 cctgcagcgc ggccaccgac tgctgcgtca gcagggtcgg cgcgctccag ccatgctcga
    13681 actgtgccgc ggcgggcgtt gccggatcca cggctgcctg catgcccagc tgctcgggca
    13741 gcgagaacca cagcggcgcc gagcgcccaa agcgcgcggc aaactcaatg gccagcatgt
    13801 cgaacggatc ctgctgaccg gtggcgcggt acaggtcgaa cagcgccagc cagacctcgt
    13861 ccggccgcgc tccgtcctgc gggggctgcg tcaggatctc cctgagcccg gcctcggcac
    13921 cctcgaagtc gccgctggcg aaacggatgg cggcctcttc gaggtcgggg tcatgctcga
    13981 acggctcggc atcggccacg agtgagggca ccgcggctgc catcgcggcg tcggccgctg
    14041 gcggaacctg caaagccccc tgtcccaggg atgcgggcat gctcatcggg gcggtgggcg
    14101 caaaggcgtg cacctggtct ccaagcgccg cttgcgctgc caacaccacg gctggccttg
    14161 gcggcacgat gggcggcgcg acgaccatgc gctgcgactc gggtgccgcg ccctgctggc
    14221 tggccgtctg gggggcggga gcgccctgct ccttgccctt ccaccattgc tgcgacatct
    14281 gggcttcgat ctcgtcgatc ttcttgagcg tgccggcacg ctcgtcgggc gaggtcatgc
    14341 tgttcgagaa aaacgaggcg cgtgccaagg cgtcgtccag gcgccggccg ccctgggcct
    14401 cgcgctggcg cagcttgcgc agctgatcga actcgcgctt gcgcacgaag tcgttgcgcc
    14461 gcttgcgctc gatcatctcc ttgagcatct gcttgctgta ctggctgtcg cggtcagttt
    14521 cgagcgagtc caggtcagac cagttgaccg tggggttgcg cacgaacttc accatcttgg
    14581 acagcagtcc accaggtgcc ggggtgtcgt ccttgctcat ggtttgaagt ggtgcgcctt
    14641 tatgcgccct caagcgcgcg ctgctccatg gtgcaggccg gcatgaaact ggcgtgcccc
    14701 aggccagggc cgccgtgcaa gggccgcccc gccgcacggg cggcgtcccc ct
//
LOCUS       DAOMTQ010000070        14656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_661_length_14656_cov_26.591877, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000070 DAOMTQ010000000
VERSION     DAOMTQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_14656_cov_26.591877"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PK518_08450"
     CDS             complement(<1..107)
                     /locus_tag="PK518_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM48985.1"
                     /translation="MASTHPSDQDLPLQGVRVLDLSRVFAGPMCAMVLA"
     gene            complement(133..555)
                     /locus_tag="PK518_08455"
     CDS             complement(133..555)
                     /locus_tag="PK518_08455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRM48986.1"
                     /translation="MSRSIEDARRVLDAQPFSRLVRAEMTQYTTQVVELRVPITEQIQ
                     QQHGFVHGGVVSYLADNALTFAGGMALGPGVVTSEYKINYVRPAVGDWLAARATAVHA
                     GRTQAVCRCDVYVLAGGTEKLCAVAQGTIALMGAKKTD"
     gene            complement(591..1397)
                     /locus_tag="PK518_08460"
     CDS             complement(591..1397)
                     /locus_tag="PK518_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRM48987.1"
                     /translation="MTDTALITRREGAVLILSNNNPAARNALTPGFYNGLTAALGAAA
                     CDPTVGAVVLTGEGGHFCSGGDLRQLIHRRELPLAERRLKLESLHNLVRALRDCPKPV
                     IMAVEGAAAGAGLSLALAGDLLVAAKNAQFSVAYVKVGLTPDGGATALLSEFVSRQVL
                     TELCLTGERVSGERMHQLGVVNRLVEPGQALAAALALAQQVAAGPELATARIKHLCRV
                     APRNTLEQQLELEALYMPLSQETEESREGINAFLEKRAPDFAKLRSSKPG"
     gene            complement(1587..2711)
                     /locus_tag="PK518_08465"
     CDS             complement(1587..2711)
                     /locus_tag="PK518_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM48988.1"
                     /translation="MNFEHNEDRRMLADALNRYLGEQYPAEYRNKVAYDQAGYSAETY
                     AKLAEIGAIGALFKEADGGFGGQGPDIALVFEALGNHLVAEPLLGALLVGRALTEAGT
                     DAQKAQLEGIIAGTLLAALAHDEPANHYELNRVATTATRDGAGWVLNGAKAVVLFGDQ
                     AQLLLVSARTSGAVGDEAGISLFLVPGDAAGVSARGYGRIDGGHAAELTLSNVKVGAD
                     ALLGAEGAGHALLERVVGFGLLALSAESLGAMDKAKDDTLEYLRTRKQFGVVIGSFQA
                     LQHRMADMLLEVEQARSAVINAAAAIDDGDRVAREKALSAGKYSVGKIGALIAEEAIQ
                     MHGGIGVTWELPLAHYAKRLVMIDHQLGDMDHHLARWMAL"
     gene            complement(2722..3918)
                     /locus_tag="PK518_08470"
     CDS             complement(2722..3918)
                     /locus_tag="PK518_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM48989.1"
                     /translation="MDLQFTPEEQAFREEVRAFLKDHLPAALSAKVKAGQRLTRQDQE
                     GWHAILNRKGWLAYHWPKEHGGTGWSAIQKFIFDDECAMAGGPRLVPFGLSMLGPVLI
                     KYGNEAQKKHWLPRILNGDDWWCQGYSEPGAGSDLASLKTTAVRQGDHYIVNGQKTWT
                     TQGQHANMMFCLVRTDRQAKAQSGISFLLIDMNQPGVEVRPIRTLDGDKEVNEVFLTD
                     VKVPLENLVGEENKGWTYAKYLLTYERTGIAGVGFSMAALEKLKVIASRVQRNGKPLS
                     QDPQFATRMAQVEIDLNNMATTNLRVIASVQGGGVPGAESSMLKIRGTVIRQELLSLI
                     RRAMGPYALPYIEEAQFAEYQEAPVGPPEAATAAAAYFNYRKLSIFGGSNEIQRNIIS
                     KMILGL"
     gene            complement(3996..5192)
                     /locus_tag="PK518_08475"
     CDS             complement(3996..5192)
                     /locus_tag="PK518_08475"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRM48990.1"
                     /translation="MREAVIVSTARTPMAKSHRGEFNVTPAAQLAAFSVRAAVERSGV
                     DPAMIEDLILGCGNPDGFQGRNLGRQAVLRAELPLTIAGTTITRYCASGLQAIAAATG
                     RIVAEGVDAMIAGGVETISGLRPGNNLPTDMDPWLVEHKPALYMAMIDTADIVAKRYA
                     ISREDQDAFALQSQQRTAAAQAADLFKDEIIPCGTRMAEKNKDTGEVTHRDVVATHDN
                     CNRASTTLEGLAKLEPVKGAGNFITAGNASQLSDGSSSCVLVEARQAERLGLQALGAL
                     RGFAVAGCEPDEMGIGPVFAVPKLLKRHGLTVDDIDLWELNEAFASQALYCQRQLGIP
                     NERLNVNGGAISIGHPFGMTGARLAGHILLEGQRRKAKDPSVKWGVVTMCIGGGQGAA
                     GLFEIF"
     gene            complement(5310..7415)
                     /locus_tag="PK518_08480"
     CDS             complement(5310..7415)
                     /locus_tag="PK518_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRM48991.1"
                     /translation="MNAQALVQTQQQGPVLVVSINNPPVNALGQGVRAGLLAAVEQLE
                     QDAALKAMLIVGQGKAFIAGADIREFGKPPMAPALPDVLNRMETGSKLVVAAIHGPAL
                     GGGLEVAMATHFRVALAGAKLGLPEVNLGLLPGAGGTQRSPRLMGVKAATELMLSGQH
                     LSAKAALAAGLVDKLAEGDDPLAAGLAYVNELLARGVPPRRTRDLAIADKAAALAELD
                     AIALESVKKTRGLFSPAKIIECVRAAVELPFEQGLKRERELFLECLNSPQRQGLVHAF
                     FAERETAKIPEAKAAAPRALASIAVIGGGTMGAGITVSALDAGLSVVMVERDAESIAR
                     GQKNVEKVYDGLIARGRMTAEAKAATMARYTPSTSYDAIKHVDLVIEAVFEDIEVKKA
                     VFKELDRVCKSGAVLATNTSYLDIDAIAAVTSRPQDVIGLHFFSPANIMKLLEIVVPS
                     KVAPDVVVTSFELAKRMKKVPVRAGVCDGFIGNRILSVYREAANYVLEDGASPYDIDA
                     AVRGFGYPMGPFQVSDLAGGDIGWATRKRKAATRDPKARYVEVADRIAERGWYGQKTG
                     RGWYLYPEGARTGQDDPEVLAIVDAERKKKGITPRAFSHDEIMRRYMAAMVNEGAKVL
                     EEGIALRPLDIDVTFLFGYGFPRWRGGPMKWADMQGLDKVLADIQEFAKEDALFWQPA
                     ALLQKLVAEGKNFDSLNQR"
     gene            7544..8461
                     /locus_tag="PK518_08485"
     CDS             7544..8461
                     /locus_tag="PK518_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM48992.1"
                     /translation="MDTNALSLLVEIVDTGNLSQAARKLKMTRANVSYHLTQLEKSLG
                     VQLIKRTTRRAEPTEVGLRLYEHGRNIRNELAAARETITSLGQELLGRVGISVPSGYG
                     QIVMSDWLIEFKRLYPGIVLDVLFENRTDHLRDEVDIAIRVISEPPLSVIARSLGPVH
                     YVACASRAYVQQHGLPATLYELRNSAIITSGVQGRQLRLAAYQGEERQEVMLEPTLIS
                     DHFPFLRQGILAGLGLGLVPDYVVQPEVASGQVLLALQEYRLSIFGSHMYLMYLPGRH
                     QTRAVRTCIDFLLAKAGHGMAPVPEEAPQ"
     gene            complement(8464..8820)
                     /locus_tag="PK518_08490"
     CDS             complement(8464..8820)
                     /locus_tag="PK518_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48993.1"
                     /translation="MILTSTFTTGLRRTLPALALAASGLALTGCVVAPAYPGDVVYAP
                     SAPPPLQTEVIPVAPSPVHVWINGYWGWGGASYAWRPGHWAMPPRPGYMWHQPRWNHG
                     PRGWSQHGGGWGPRRH"
     gene            complement(8916..9785)
                     /gene="htpX"
                     /locus_tag="PK518_08495"
     CDS             complement(8916..9785)
                     /gene="htpX"
                     /locus_tag="PK518_08495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRM48994.1"
                     /translation="MKRIALFLLTNIAVVAVLGIVASLLGVNRYLTANGLNLGALLGF
                     AFIMGFGGAIISLLMSKPMAKMSMGVQIINEPRNQDEAWIVETVRGFADKAGIQMPEV
                     GIYEGDPNAFATGAFKNSALVAVSTGLLQGMTREEVEAVIGHEVAHVANGDMVTMALI
                     QGVMNTFVVFLSRVIGYAVDSFLRKNDEQSSGPGIGYMVTTIVLDILLGFLAAIIVAW
                     FSRQREFRADAGAAQLMGRKQPMINALYRLGGMHPGEMPKSLQAMGITGGIGKLFSSH
                     PPIEERVAALQQG"
     gene            complement(9872..10657)
                     /locus_tag="PK518_08500"
     CDS             complement(9872..10657)
                     /locus_tag="PK518_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HRM48995.1"
                     /translation="MAAGGLGAIDWHAPWLAPLAEAGRRATQRVQAGACVATALNALV
                     DEGAVPAPGVRFVPQAALPAGMAYEQFIHDRRSVPTRDNLHDFFNGLVWLRYPATKRR
                     FNQLQALALAQDGVGAVRGPVRDALTVFDENGALLLAPPELHAALAARQWRRLCVELR
                     PLWRQARLLILGHALLEKLVSPRKSITAHVYQARSAIDNVVNLDDALARDLTAPWLAA
                     KPFCPLPVLGVPGWWPENENFCFYDDSVVFRGARQLTQAHPNA"
     gene            complement(10639..11682)
                     /gene="pyrC"
                     /locus_tag="PK518_08505"
     CDS             complement(10639..11682)
                     /gene="pyrC"
                     /locus_tag="PK518_08505"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRM48996.1"
                     /translation="MTATTNTLTITRPDDWHLHVRDGEPLLTVVPHTAAQFGRAIIMP
                     NLRPPVTTAQQALEYKQRILAAVPAGMSFEPLMTLYLTDNLAPDEIARAKDAGVVALK
                     LYPAGATTNSDAGVTDIRKTYKTLEAMQRAGLLLLVHGEVTSPDIDLFDREAVFIDQQ
                     LIPLRRDFPELKIVFEHITTREAAQYVMGSDAHTAATITAHHLLYNRNAIFTGGIRPH
                     YYCLPVLKRELHRQALVEAATSGSAKFFLGTDSAPHPAHLKEHATGCAGCYTAHAAIE
                     MYAEAFDNAGALDRLEGFASFHGPAFYGLPRNPGQITLRRESWTPPDSFVFGEAELKP
                     LRAGEALPWRLVA"
     gene            complement(11845..12171)
                     /locus_tag="PK518_08510"
     CDS             complement(11845..12171)
                     /locus_tag="PK518_08510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM48997.1"
                     /translation="MMRGDLVTIALQGDFGKPRPALVIQADLFDAHASVTLLPITSTL
                     VAAPLLRVPLTPSAGNGLQKPSQVMVDKAMTVSRDKLGPSIGRIDPDKLVEVERCLAV
                     FLGIAK"
     gene            complement(12168..12392)
                     /locus_tag="PK518_08515"
     CDS             complement(12168..12392)
                     /locus_tag="PK518_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HRM48998.1"
                     /translation="MSTSTSNARVQRHRDALRQAGLRPVQLWVPDTRRPDFAQECRRQ
                     SLLASQADAADAALVPLMDAALADVDGWTD"
     gene            complement(12463..12828)
                     /locus_tag="PK518_08520"
     CDS             complement(12463..12828)
                     /locus_tag="PK518_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM48999.1"
                     /translation="MPQPPLPAGWQRVHWQPQAPAKPALGQPCNGCGLCCLAEPCPLG
                     IWASRRRSGPCAALRWDTGQGRYLCGVLADATQAAATQGGWRRRLWLALVRRWIAAGQ
                     GCDADLQVLDVLGEKAKRP"
     gene            complement(12832..13137)
                     /locus_tag="PK518_08525"
     CDS             complement(12832..13137)
                     /locus_tag="PK518_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HRM49000.1"
                     /translation="MAFVLQVDFSMDGPFGADMVQAFEGLARSINQEPGMRWKIWTED
                     AQAREAGGIYLFDTREHAQRYLDMHAQRLTQAGVRNIRSRIYAVNAALTAINQGPVA"
     gene            13284..13673
                     /locus_tag="PK518_08530"
     CDS             13284..13673
                     /locus_tag="PK518_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRM49001.1"
                     /translation="MNKTIPASHVRIKRAYEAPTPADGARILIDRLWPRGVKKEALLL
                     AEWAKPLAPSTELRQWFNHDPALWDEFRRRYAAELRQQPEAWAQLRERARHGVVTLVY
                     SAHDEAVNNAVAMRDFLLQPGGLDAAQ"
     gene            13693..14154
                     /locus_tag="PK518_08535"
     CDS             13693..14154
                     /locus_tag="PK518_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRM49002.1"
                     /translation="MTQRLDYKQASPKGFAAMLGLEQHARSSGLDHALLELVKTRVSQ
                     INGCAFCLDMHSKDARAAGEDEQRLYLLAAWREAPCYSPRERAALAWAEAVTLLKDQA
                     VGDAQYHAAREHFDDKALVDLTLAIVAINGWNRLSIAFRTPAGTYQPQHHS"
     gene            14173..14508
                     /locus_tag="PK518_08540"
     CDS             14173..14508
                     /locus_tag="PK518_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRM49003.1"
                     /translation="MDQAMYPASSADIARKRRELAPKQLEAFRQFSAAAFAEGELDAR
                     TKQLIAVAVAHVTQCPYCIRGHTEGALKAGASEAQIMEAIWVAAEMRAGGAYAHSALA
                     IDTMAGHKG"
BASE COUNT         2333 a         4883 c         4986 g         2454 t
ORIGIN      
        1 tcggccagca ccatggcgca catggggccg gcaaacacgc gcgagagatc cagcacgcgc
       61 acgccttgca ggggtaagtc ttggtcggac ggatgggtcg atgccatggc tcgcattcct
      121 ttgttgctca agtcagtcgg ttttcttcgc gcccatcagg gcaatcgttc cctgtgcgac
      181 agcgcacagc ttttcagtgc cgccagcgag cacatagaca tcgcaacggc agacagcctg
      241 tgtacggcct gcatgcaccg ccgtggcgcg tgcggcaagc caatcgccga cggcgggacg
      301 cacatagttg atcttgtatt ccgaggtcac cacaccaggc cccagtgcca tgccacccgc
      361 aaaggtgagc gcgttatccg ccaggtacga gaccacaccg ccatgcacaa agccgtgttg
      421 ctgctggatc tgttcggtga tgggcacacg cagctccacc acctgcgtcg tgtactgcgt
      481 catttctgcc cggaccaggc gactgaacgg ctgcgcgtcc agaacccgtc gcgcgtcttc
      541 aatggatcgg ctcatacgtc catccctcct tatgcaaggc gcaccggcgc tcagcccggc
      601 ttgctgctgc gcagcttggc aaaatcgggc gcgcgctttt ccaggaaggc gttgatgccc
      661 tcgcgcgact cctcggtctc ttgcgacagc ggcatgtaca gcgcctccag ctccagctgc
      721 tgctccagcg tgttgcgcgg cgccacgcgg cacaggtgct tgatgcgcgc ggtggccagc
      781 tcggggccgg cggccacctg ctgcgccagc gccaaagccg cggccagcgc ctggccgggc
      841 tcgacgaggc gattcaccac gcccagttgg tgcatgcgct cgccgctcac gcgttcgccg
      901 gtcaggcaca gctcggtcaa gacctggcgc gagacgaatt cggacagcag cgccgtggcg
      961 ccgccgtcgg gcgtgaggcc caccttgacg taggccaccg agaactgcgc gttcttcgcg
     1021 gccaccagca ggtcgccggc cagcgccagc gacaggccgg caccggcggc cgcgccctcc
     1081 accgccatga tcacgggctt ggggcagtcg cgcagcgcgc gcaccaggtt gtgcaggctt
     1141 tcgagcttca ggcgccgctc ggccaggggc agctcgcgcc ggtggatgag ctggcgcagg
     1201 tcgccgccgg agcagaaatg cccgccctcg ccggtgagca ccacggcgcc cacggtcggg
     1261 tcgcaggccg ccgcgcccag ggccgccgtc aggccgttat agaagcccgg cgtgagcgcg
     1321 ttgcgcgcgg ccgggttgtt gttggacagg atgagcaccg cgccttcgcg gcgggtgatc
     1381 agggcggtgt cggtcattta tgtctcctgc tgtgcgcttt tgttttgata gctggtggcg
     1441 cttgatggat aagcgccaag gcctgttttt gctttaactc cctctcccgc gtgcgggaga
     1501 gggtgggggt gagggtgctt gccgtgcaag cacagcgccc aaactacccc ctcacccctg
     1561 ccctctcccc caagggggcg agggagttac aacgccatcc agcgcgccag gtggtggtcc
     1621 atgtcgccga gctggtggtc gatcatcacc aggcgcttgg cgtagtgggc cagcggcagc
     1681 tcccaggtca cgccgatgcc gccgtgcatc tggatcgcct cctcggcgat cagcgcaccg
     1741 atcttgccca cgctgtactt gccggcgctc agggccttct cgcgcgccac gcggtcacca
     1801 tcatcaatgg cggcagcggc gttgatcact gccgagcgcg cctgctccac ctccagcagc
     1861 atgtcggcca tgcggtgctg cagggcctgg aagctgccga tcaccacgcc gaactgcttg
     1921 cgcgtgcgca ggtattccag cgtgtcgtcc ttggccttgt ccatggcgcc caggctctcc
     1981 gccgacagcg ccagcaggcc gaagcccacc acgcgctcca gcagcgcatg gccggcgccc
     2041 tcggcgccca gcagcgcgtc ggcaccgacc ttgacgttgg acagggtcag ctcggccgca
     2101 tggccgccgt cgatgcggcc atagccgcgc gccgagacgc cggccgcgtc gcccggcacc
     2161 aggaacagcg aaatgccggc ctcgtcgccc accgcgcccg aagtgcgcgc gctcaccagc
     2221 agcagctgcg cctggtcgcc aaacagcacg acagccttgg cgccgttcag cacccagcct
     2281 gcgccgtctc tcgttgcggt ggtggcgacg cggttcagct cgtagtggtt ggccggctcg
     2341 tcatgcgcca gggccgcaag cagggtgccg gcgatgatgc cttcgagctg cgccttctgc
     2401 gcgtcggtgc cggcctcggt cagcgcccgg cccaccagca gcgcgcccag caggggctcg
     2461 gccaccaggt gattgcccag ggcctcgaac accagggcga tgtcgggacc ctggccgccg
     2521 aagccgccat cggcctcctt gaacagcgcg ccgatggcgc cgatctcggc cagtttggcg
     2581 taggtctcgg cgctgtagcc ggcctggtcg taggccacct tgttgcggta ttcggcgggg
     2641 tactgctcgc ccaagtagcg gttcagcgca tccgccagca tgcggcggtc ttcgttgtgc
     2701 tcaaagttca tggcgattcc cttacagccc caggatcatc ttggagatga tgttgcgctg
     2761 gatctcgttg gagccgccga agatcgacag cttgcggtag ttgaaatagg cggccgcggc
     2821 cgtggcggcc tcgggcgggc ccaccggcgc ctcctggtat tcggcgaact gcgcctcctc
     2881 gatgtagggc agcgcgtacg gccccatggc gcggcggatc agcgacaaca gctcctggcg
     2941 gatcaccgtg ccgcggatct tgagcatgga gctctcggcg cccggcacgc ccccgccctg
     3001 caccgacgca atgacgcgca ggttggtcgt ggccatgttg ttcaggtcga tctccacctg
     3061 cgccatgcgg gtggcgaact gcgggtcctg gctcaaaggc ttgccgttcc tttgcacgcg
     3121 gctggcaatc accttgagct tttccagcgc ggccatcgag aagcccacgc cggcgatgcc
     3181 ggtgcgctcg taggtcagca ggtacttggc gtaggtccag cccttgttct cctcgccgac
     3241 caggttctcc aacggcacct tcacgtcggt gaggaagacc tcgttgacct ccttgtcgcc
     3301 gtccagcgtg cggatcgggc gcacctcgac gccgggctgg ttcatgtcga tgagcaggaa
     3361 gctgatgccc gactgcgcct tggcctggcg gtcggtgcgc accagacaga acatcatgtt
     3421 ggcgtgctgg ccctgggtgg tccaggtctt ctggccgttg acgatgtagt ggtcaccctg
     3481 gcgcacggcg gtggtcttga ggctggccag gtcagagccg gcgcccggtt cggaatagcc
     3541 ctggcaccac cagtcgtcgc cattgaggat gcgcggcagc cagtgttttt tctgcgcctc
     3601 gttgccgtac ttgatcagca ccgggccgag catggacagg ccgaacggca ccaggcgcgg
     3661 gccgccggcc atggcgcact cgtcgtcgaa gatgaatttc tggatggcgc tccagccggt
     3721 gccgccatgc tccttgggcc agtgataggc cagccagccc ttgcggttca ggatggcgtg
     3781 ccagccctct tggtcctggc gcgtcaggcg ctgcccggcc ttgaccttgg cggagagcgc
     3841 cgcgggcagg tggtccttca gaaacgcacg cacttcctcg cggaaggcct gctcctcagg
     3901 ggtgaattgc aaatccatgt cttgcctcta gtcagttggg gacagggcaa aaattgcgct
     3961 gcaatttttg ggcctgtcgc acaaggtttc ggttttcaga aaatctcgaa cagtccggcc
     4021 gcgccctggc cgccgccgat gcacatggtc accacgcccc acttcacgga cgggtccttg
     4081 gccttgcggc gctggccttc cagcaggatg tggccggcca ggcgcgcacc cgtcatgccg
     4141 aaggggtggc cgatggagat ggcgccgcca ttgacattca ggcgttcgtt cggaataccc
     4201 agctggcgct ggcagtacag ggcctgcgag gcaaaggcct cgttcagctc ccacaggtct
     4261 atgtcgtcca cggtcagacc atggcgcttg agcagcttgg gcacggcgaa caccgggcct
     4321 atgcccattt catccggctc gcacccggcc acggcaaagc cgcgcaacgc gcccagggcc
     4381 tgcaggccca ggcgctcggc ctgccgggcc tcgaccagca cgcaggacga ggagccatcg
     4441 gacagctgcg aggcattgcc cgcggtgatg aagttgcccg cgcccttgac cggctccagc
     4501 ttggccaggc cttcgagcgt ggtcgaggcg cggttgcagt tgtcgtgcgt ggccaccacg
     4561 tcgcggtggg tgacctcgcc ggtgtccttg ttcttctcgg ccatgcgcgt gccgcagggg
     4621 atgatctcgt ccttgaacag gtcggcggcc tgggcggcgg cggtgcgctg ctggctttgc
     4681 agcgcaaacg catcctgatc ctcgcggctg atggcatagc gcttggccac gatgtccgcc
     4741 gtgtcaatca tggccatgta cagcgcaggc ttgtgctcga ccagccaggg gtccatgtcg
     4801 gtgggcaggt tgttgcccgg gcgcaggccc gagatggtct ccaccccgcc ggcgatcatg
     4861 gcgtccacgc cctcggccac gatgcggccg gtggcggcgg caatcgcctg caggcccgag
     4921 gcgcagtagc gggtgatggt ggtgccggcg atggtgaggg gcagctcggc gcgcagcacg
     4981 gcctggcggc ccaggttgcg gccctggaaa ccgtcggggt tgccgcagcc caggatcaag
     5041 tcctcgatca tggccggatc gacgcccgag cgctcgaccg cggccctgac cgagaaggcc
     5101 gccagctggg cggcaggggt cacgttgaat tcgccgcggt gcgacttggc cataggcgta
     5161 cgggctgtgg aaacaatgac ggcttcgcgc atggggtgct cctttggaac ggtttgtctt
     5221 tatggaatca atagctgctt gcgcagtgtg tacgggcgct tgaggctttt tctccctctc
     5281 ccccaagggg gagagggagt tggcgagggt cagcgctggt tcaggctgtc gaagttcttg
     5341 ccctcggcca ccagcttttg cagcagtgcg gccggttgcc agaacagcgc gtcttccttg
     5401 gcaaattcct ggatgtcggc caaaaccttg tccaggccct gcatgtcggc ccatttcatc
     5461 gggccgccgc gccagcgcgg gaagccgtag ccaaacaaaa aggtcacgtc gatatccagc
     5521 gggcgcaggg caatgccctc ctccagcacc ttggcgccct cgttgaccat ggccgccata
     5581 tagcggcgca tgatttcgtc atggctgaag gcgcgcggcg tgatgccctt ctttttccgc
     5641 tcggcatcga cgatggccag cacctcgggg tcgtcctggc cggtgcgtgc tccttcaggg
     5701 tacaggtacc agccgcgccc ggtcttctgg ccataccagc cgcgctcggc gatgcggtcg
     5761 gccacctcga cgtagcgcgc cttggggtcg cgcgtggcgg ccttgcgctt gcgcgtggcc
     5821 cagccaatgt cgccgccggc cagatcgctc acctggaacg gccccatggg gtagccaaag
     5881 ccgcgcaccg cggcgtcgat gtcgtagggg ctggcgccgt cctccagcac gtagttggcc
     5941 gcctcgcggt agaccgacag gatgcggttg ccgatgaagc cgtcgcacac gccggcgcgc
     6001 accggcacct tcttcatgcg cttggccagc tcgaacgagg tcaccaccac gtcgggcgcc
     6061 accttcgatg gcacgacgat ctccagcagc ttcatgatgt tggccgggct gaagaaatgc
     6121 aggccgatca cgtcctgcgg gcgactggtc accgcggcga tggcgtctat gtccaggtac
     6181 gaggtgttgg tggccagcac ggcgccgctc ttgcagacgc ggtcgagttc cttgaacacc
     6241 gccttcttca cctcgatgtc ctcgaacaca gcctcgatca ccaagtccac atgcttgatg
     6301 gcgtcgtagc tggtgctggg cgtgtagcgc gccatggtgg cggccttggc ctccgccgtc
     6361 atgcggcccc tggcgatcag gccgtcgtag accttctcca cgtttttctg gccgcgtgcg
     6421 atggactcgg catcgcgctc caccatcacc acggacaggc cggcatccag cgccgagacg
     6481 gtgatgcccg cgcccatggt gccgccgccg atcacggcga tgctggccag cgcacgcggc
     6541 gcagcggcct tggcttcggg aatcttggcg gtctcgcgct cggcgaagaa ggcatgcacc
     6601 aggccctggc gctgcgggct gttcaggcat tccagaaaca gttcgcgctc acgcttgagg
     6661 ccctgctcga aaggcagctc gacggccgcg cgcacgcatt cgatgatctt ggcgggcgag
     6721 aacaggccgc gcgtcttctt gacgctttca agagcgatgg cgtccagctc ggccagggcc
     6781 gcggccttgt cggcaatggc aaggtcgcgc gtgcgccggg gcggcacgcc gcgcgccagc
     6841 agctcgttca cataggccag gccggcggcc agcgggtcgt cgccctcggc cagcttgtcc
     6901 accaggccgg cggccagggc cgccttggcc gacaggtgct ggccgctgag catcagctcg
     6961 gtcgcggcct tcacgcccat caggcgcggc gagcgctgcg tgccgccggc gccgggcagc
     7021 aggcccagat tcacctcggg caggcccagt ttggcaccgg ccagggcgac acggaaatgc
     7081 gtggccatgg cgacttcgag cccaccgccc agggccgggc cgtggatggc ggcgaccacc
     7141 agcttggagc ccgtctccat gcgattgagc acatcgggca gcgcgggcgc catgggcggc
     7201 ttgccgaact cgcggatgtc ggcgccggcg atgaaggcct tgccctggcc gacgatgagc
     7261 atggccttga gcgccgcgtc ctgctccagc tgctccacag ccgccagcag gccggcacgc
     7321 acgccctggc ccagggcgtt gacgggaggg ttgttgatgc tgacaaccag caccgggcct
     7381 tgttgctggg tctgtaccaa tgcttgtgcg ttcatgcgct tgtgtctccg agggtctggt
     7441 gaagattggc attgtgggaa cgcgagcgcg gtttgacaat cccatgaagc attgacagac
     7501 tgtcaaatca ttttagacaa ttaccaagga ttaaggagga tccatggaca ccaatgcgct
     7561 aagcctgctg gtcgagatcg tggacaccgg caacctgagc caggccgcgc gcaagctgaa
     7621 gatgacccgc gccaacgtca gctaccacct tacgcagctg gaaaagtcgc tgggcgtgca
     7681 gctcatcaag cgcaccacgc gccgcgccga gcccaccgag gtgggcctgc gcctgtacga
     7741 gcatggccgc aacatacgca acgaactggc cgccgcgcgc gagaccatca ccagcctggg
     7801 ccaggagctg ctggggcgcg tgggcatcag cgtgcccagc ggctacgggc agatcgtgat
     7861 gagcgactgg ctgatcgagt tcaagcggct ctaccccggc atcgtgctcg acgtgctgtt
     7921 cgagaaccgc accgaccacc tgcgcgacga ggtggacatc gccatccgcg tgatctccga
     7981 gccgccgctg tcggtgatcg cgcgcagcct gggcccggtg cactatgtgg cctgcgcctc
     8041 acgcgcctat gtgcagcagc acggtctgcc ggccacgctg tacgagctac gcaacagcgc
     8101 catcatcacc tcgggcgtgc aggggcgcca gctgcgcctg gcggcctacc agggcgagga
     8161 gcgccaggag gtgatgctgg agccgacgct gatctcggac cattttccgt tcttgcgcca
     8221 gggcatattg gccggcctgg gcctgggcct ggtgcccgac tacgtggtgc agcccgaggt
     8281 ggccagcggc caggtgctgc tggcgctgca ggaataccgg ctcagcatct ttggcagcca
     8341 catgtatctg atgtacctgc ccggccgcca ccagacccgc gccgtgcgca cctgcataga
     8401 ctttctgctg gccaaggccg ggcatggcat ggcgcccgtc ccggaggagg cgccacagtg
     8461 acatcaatgg cggcgtggcc cccagccccc gccatgctgg ctccagccgc gcggcccatg
     8521 gttccagcgg ggctggtgcc acatatagcc cggccgcggc ggcatggccc agtggccggg
     8581 gcgccaggca tagctggcac caccccagcc ccagtagccg ttgatccaca catgcaccgg
     8641 cgacggcgcc acgggaatca cctcggtctg cagcggcggc ggcgcgctgg gcgcatacac
     8701 cacgtcgccc ggataggccg gcgccaccac gcagccggtc agcgccaggc ccgatgcggc
     8761 cagcgccagg gccggcagcg tgcggcgcag gccggtggtg aaagtggacg tgaggatcat
     8821 gttgctctcc tggcatgcaa ggcacatgca catgctccta caacgaaaaa gcccctgtcg
     8881 cttgttgaca ggggcttgtg aagccaaggt aaagatcagc cctgctgcag cgcggccacg
     8941 cgctcctcga tgggcgggtg cgagctgaac agcttgccga tgccgccggt aatgcccatg
     9001 gcctgcaggc tcttgggcat ctcgccgggg tgcatgccgc ccaggcggta cagggcattg
     9061 atcatgggct gcttgcggcc catgagctgg gcggcgccgg cgtcggcgcg gaactcgcgc
     9121 tggcgcgaga accaggccac gatgatggcc gccaggaagc ccagcaggat gtccagcacg
     9181 atggtggtga ccatgtagcc gatgccgggg cccgagctct gctcgtcgtt cttgcgcaga
     9241 aagctgtcca ccgcgtagcc gatgacccgc gacaggaaaa cgacgaaggt attcatcacc
     9301 ccctggataa gcgccatggt gaccatgtcg ccattggcca cgtgggccac ctcgtggccg
     9361 atgacggcct cgacctcctc gcgcgtcatg ccctgcaaga ggccggtgga cacggccacc
     9421 agggccgagt tcttgaaggc gccggtggca aaggcgttcg ggtcgccctc gtagatgccg
     9481 acctcgggca tctgtatgcc ggccttgtcg gcaaagccgc gcacggtctc gacgatccag
     9541 gcctcatcct ggttgcgcgg ctcgttgatg atttgcacgc ccatgctcat cttggccatg
     9601 ggcttgctca tcagcagcga aatgatggcg ccgccaaagc ccatgatgaa ggcaaagccc
     9661 agcagcgccc ccaggttcag gccgttggcc gtcaggtagc ggttcacgcc cagcaggctg
     9721 gcgactatgc ccagcactgc caccacggcg atgttggtca acaaaaagag cgcaatgcgt
     9781 ttcatcgaga gtgttctcag gtgaagggtt cgggtgggcc cgcggcaggc cgccttgggc
     9841 agaaaacaag atggggacgg tgcgcggcgc ttcaagcgtt ggggtgcgcc tgtgtcagct
     9901 gtcgtgcgcc acggaaaacg acagagtcat cataaaagca aaagttctcg ttctcgggcc
     9961 accagccggg cacgcccagc accggcaagg ggcaaaaggg tttggcggcc agccatggtg
    10021 ccgtcaggtc gcgggccaga gcatcgtcca gattcactac attatcaata gctgatcgcg
    10081 cttgatacac atgggccgtg atcgattttc tgggagaaac cagcttttcc agcagcgcat
    10141 ggcccaggat caacagccgc gcctggcgcc acagcgggcg cagctccacg cacagccggc
    10201 gccattggcg cgcagccagc gcggcatgca gctcaggcgg cgccaacagc aaggcaccgt
    10261 tctcgtcgaa caccgtgagc gcatcgcgca ccggcccgcg cacggccccc acgccatctt
    10321 gggcaagcgc cagcgcctgc aactggttga agcgccgctt ggtggctggg tagcgcagcc
    10381 acaccagacc gttgaaaaag tcatgcaggt tgtcgcgcgt gggcacgctg cgccggtcgt
    10441 ggatgaactg ctcgtaagcc atgcccgccg gcagcgccgc ctgcggcacg aagcgcacgc
    10501 cgggcgccgg gactgcgccc tcgtcgacca gcgcattcag cgcagtggcc acgcaggcgc
    10561 cggcctgcac gcgctgggtc gcccggcgcc ctgcctcggc cagcggcgcc agccaggggg
    10621 cgtgccagtc aatggcgcct aggccaccag ccgccacggc agcgcctcgc ccgcgcgcag
    10681 aggcttgagc tcggcctcgc cgaaaacgaa gctgtcgggc ggcgtccagc tttcgcggcg
    10741 cagcgtgatc tgccccgggt tgcgcggcag gccgtagaag gccgggccgt ggaagctggc
    10801 aaagccctcc agcctgtcca gcgcgccggc gttgtcgaag gcctcggcgt acatctcgat
    10861 ggcggcgtgc gcggtgtagc agccggcgca gcccgtggcg tgctccttca ggtgcgcggg
    10921 gtggggtgcg ctgtcagtgc ccaggaagaa cttggcgctg ccgctggtgg ccgcctcgac
    10981 caacgcctgg cggtgcagct cgcgcttgag caccggcagg cagtagtagt gcgggcgaat
    11041 gccgccggtg aagatggcgt tgcggttgta cagcaggtgg tgggcggtga tggtggcggc
    11101 ggtgtgcgca tcgctgccca tcacatactg cgcggcctcg cgcgtggtga tgtgctcgaa
    11161 gacgatcttc agctcgggga agtcgcggcg cagcgggatc agctgctggt ctatgaacac
    11221 cgcctcgcgg tcgaacaggt cgatgtcggg gctggtcacc tcgccatgca ccaagagcag
    11281 caggccggcc ctttgcatgg cctccagcgt tttgtaggtc ttgcggatat cggtcacgcc
    11341 ggcgtcgctg ttggtggtgg cgccggccgg gtagagcttg agggccacca caccggcatc
    11401 cttggcacgg gcgatttcat cgggcgccag gttgtcggtg aggtacagcg tcatcagggg
    11461 ctcgaagctc atgccggcgg gcacggcggc caggatgcgc tgcttgtatt ccagcgcctg
    11521 ctgcgccgtg gtcacgggcg ggcgcaggtt gggcatgatg atggcgcggc cgaactgcgc
    11581 ggccgtgtgt ggcacgacgg tcagcaaagg ctcgccgtcg cgcacatgca ggtgccagtc
    11641 gtcggggcgg gtgatggtga gggtgttcgt ggtggcggtc ataccggcga ttgtcccatt
    11701 cagacagtgc ctttaccgcg ctgatcaagt agcgagcctt gccggcatat tcaagaagcc
    11761 gaagacgccc ctcctcccac caaaactctc gccccaaacg aaggcttcaa gtcctgcgtt
    11821 gctgttctgc gggcatccaa gacatcactt ggcaatcccc aagaaaaccg ccaggcagcg
    11881 ctccacctcc accagtttgt cggggtcgat gcggccaatg ctggggccga gcttgtcgcg
    11941 cgacaccgtc atggccttgt ccaccatgac ctgcgagggc ttttgcaagc cgttgcctgc
    12001 gctgggcgtg agcggcacgc gcagcagcgg cgcggccacc aaggtgctgg tgatgggcaa
    12061 cagcgtgacg ctggcgtgcg catcaaacaa atcggcctgg atgacgagcg cgggccgggg
    12121 tttgccaaaa tcgccctgca aggcaatggt caccaggtcg ccgcgcatca atccgtccag
    12181 ccgtccacat cggccagggc ggcatccatc agcggcacca gcgcggcatc ggccgcgtcg
    12241 gcctgagagg ccagcaggct ttggcggcgg cattcctggg caaaatcggg gcggcgggta
    12301 tcgggcaccc acagctgtac cgggcgcagc ccggcctggc gcagcgcgtc acgatggcgc
    12361 tgcacgcggg cattgctggt ggatgtggac attgggtgct cttgaagatc tggcgttaca
    12421 tgcaacatga tagggtcgtt gcatgtagcg cacaagcccg agtcaaggtc gttttgcctt
    12481 ttctccaagt acatccagca cctgcaaatc ggcatcgcag ccctggcccg cggcaatcca
    12541 gcgccgcacc agggccagcc agagccggcg gcgccagccg ccctgggtgg ctgcggcctg
    12601 cgtggcgtcg gccagcacgc cgcagaggta gcgcccctgc cccgtgtccc agcgcagtgc
    12661 cgcgcagggg ccgctgcgcc ggcgcgatgc ccagatgcca agcgggcagg gctcggccag
    12721 gcagcacagg ccgcagccgt tgcagggttg gcccagcgcg ggcttggcgg gtgcctgggg
    12781 ctgccagtgc acgcgctgcc agccggcggg caggggcggc tgaggcatgc gctacgccac
    12841 cggcccctgg ttgatggccg tgagcgcagc gttgacggcg tagatgcggc tgcggatgtt
    12901 gcgcacgccg gcctgcgtga ggcgctgtgc gtgcatgtcc aggtagcgct gggcgtgctc
    12961 ccgcgtatcg aacaggtaga tgccgccggc ctcgcgggcc tgggcgtcct cggtccagat
    13021 cttccagcgc atgccgggct cctgattgat gctgcgcgcc aggccctcga aggcctggac
    13081 catgtcggcg ccaaaggggc cgtccatgga gaaatcgact tgcaggacaa aggccatgtg
    13141 cactccttgt gtggccagtg atgcggccgg tggtggtggg gcgattgtcc catcggccca
    13201 gcggccaata cccctgctgg tggctgcagg ccggggcggg cgtatcctgc cccggtgtcg
    13261 ggttgcaaca gcaaggccat gccatgaaca agaccatccc cgcctcccat gtgcgcatca
    13321 agcgcgccta cgaggccccc acgccagcgg acggcgcacg catcctgatt gaccggctgt
    13381 ggccgcgcgg cgtgaagaag gaggcgctgc tgcttgccga atgggccaag ccgctggcgc
    13441 caagtaccga gctgcgccag tggttcaacc acgaccccgc actgtgggac gaattccgcc
    13501 gccggtacgc agcggagttg cgccagcagc ccgaggcctg ggcgcagctg cgcgagcgcg
    13561 cccgccatgg cgtggtgacg ctggtgtatt cggcgcatga cgaggcggtg aacaacgccg
    13621 tcgccatgcg cgattttttg ctgcagcccg gcgggctgga tgcggcgcaa tgacggcaac
    13681 cggaggtatt caatgaccca acgactggac tacaagcagg catcgcccaa gggctttgcc
    13741 gccatgctgg ggctggagca gcacgcgcgc agcagcgggc tggatcacgc gctgctggag
    13801 ctggtgaaga cgcgcgtctc gcagatcaac ggctgcgcct tctgcctgga catgcacagc
    13861 aaggacgcgc gcgccgccgg cgaggacgag cagcgcctgt atctgctggc cgcctggcgc
    13921 gaggcgccct gctactcgcc gcgcgagcgc gccgccctgg cctgggccga ggcggtgacg
    13981 ctgttgaaag accaggcggt gggcgacgcc cagtaccacg cggcgcgcga gcatttcgac
    14041 gacaaggcgc tggtggacct gacgctggcc atcgtcgcca tcaacggctg gaaccggctg
    14101 tcgatcgcgt tccgcacgcc ggccggaacc taccaacccc agcaccacag ttgactaccg
    14161 gaggacagca ccatggacca agccatgtac ccggccagca gtgccgacat cgcacgcaag
    14221 cgccgcgagc tggcgcccaa gcagctggag gccttccgcc agttcagcgc ggcggcgttt
    14281 gccgaaggcg agctggacgc gcgcaccaag caactgatcg ccgtggccgt ggcccatgtc
    14341 acgcagtgcc cgtactgcat tcgcggccac accgagggcg cgctcaaggc tggcgccagc
    14401 gaggcgcaaa tcatggaggc catctgggtc gcggccgaga tgcgcgcggg cggcgcctat
    14461 gcgcattcgg cgctggcgat cgacacgatg gcggggcaca agggctgaac cgcgcggcgg
    14521 gtacatgccg gcgagacgcc ggcgctcccg gtaccgccac cgcacaaaga tttgcgcgca
    14581 cacattttta tagcgtgttg tgcagcgtgg gcgctggttt ttggctgttt ctccctctcc
    14641 cgcgtgcggg agaggg
//
LOCUS       DAOMTQ010000071        14645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_662_length_14645_cov_23.778615, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000071 DAOMTQ010000000
VERSION     DAOMTQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14645
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_14645_cov_23.778615"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PK518_08545"
     CDS             <1..167
                     /locus_tag="PK518_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM49004.1"
                     /translation="YSITVPGVVPKLSATPGTIRTSAPRLGDDTDAVLAEAGLTAEQI
                     ALLRSKGVIQ"
     gene            164..1138
                     /locus_tag="PK518_08550"
     CDS             164..1138
                     /locus_tag="PK518_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRM49005.1"
                     /translation="MTTSVWNGAGRRIELCDVGLRDGLQMEAQFVPTEDKIALVNALS
                     QAGLSKIEVTSFTSPTAIPALRDAEVVMREIERRPGVTYTALVPNARGAERAIESRTD
                     ELNLVMSVSETHNLANLRMTRAQSFAALAQVITMAQAAQVPVNVSLSCVFGCPMEGDV
                     ALDAVWDHVQRFADLGVAGITLCDTTGMAFPTQVGQVVAAARARWPGMAFTLHFHNTR
                     GMGLANVLAAIDAGAQRFDASLGGLGGCPYAPGATGNVCTEEVVHALQLMGYDTGIDL
                     PALIATAARLPALIGHEVPSQIVKAGCRLDLHPVPAGFDAIRERALAR"
     gene            1176..2177
                     /locus_tag="PK518_08555"
     CDS             1176..2177
                     /locus_tag="PK518_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49006.1"
                     /translation="MPLTTASHGRRAALAFGAAVGALLCTAPLGAAAQDKYPSKPITL
                     VVPQAAGGANDAIARVLAQRLSEQMGQSVVVDNRPGAGGTLATAALARAKHDGYTLLV
                     TADSAHVIGPALYKNPGFDAVKDFEPVAPVATAGYVLVAHPSFPGNNVADLIRLAKAS
                     PGKYAIASAGNGTLNHLIGELLQKAAGIQLQHIPYKGSAAAATDVAGGQVPLSVQSLP
                     SSIAFIKAGKLKVLGVVNEKRVAALPDVPTIGETLKGFGQAPWYAMFAPAGTPAPIVE
                     RLQAEVARALDHKDVVDKLAVVGCEPFKGSSAQLGALVQSDLPKWARVVKDTGATVD"
     gene            2231..3217
                     /locus_tag="PK518_08560"
     CDS             2231..3217
                     /locus_tag="PK518_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49007.1"
                     /translation="MTTRITRKHFGALALAALAPLALSTAWAQGGYPSRTVTFVVPAA
                     AGGTTDISARMAAQALAPVLGQSVVVDNKGGGNGAIAANLVKRAEPDGYTLLMQYSGY
                     HVISPHLTRAPQWEQKDFQPIANVISAPQIIVVRESLPVKTLAELVAYAKANPGKLNY
                     ASSGNGSLQHVTGAMLEQQGGIKMVHVPYKGTGPALQDLLGGQVDLTFGTAPPFMPHI
                     QSGKLRVLAVTGKERLASLPDVPTTAEAGYAGVNATSWFALFAPAGVPKPVIDKLAAD
                     MKKVVEDPAFRKKAQEQGAAADYQSPQQLGERVRSDFANWAGVIKSSKIEAE"
     gene            complement(3233..4159)
                     /locus_tag="PK518_08565"
     CDS             complement(3233..4159)
                     /locus_tag="PK518_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM49008.1"
                     /translation="MINISLRQLEYFAAAAQHGGAARAAEALSVSQPSISKAIADLEA
                     LWGETLFVRRHARGLELTAAGQMRHREAHALLERARALEGPRTDEEAGLLRVGCLATL
                     APRHLPAMLLRMRERHPRVEIEVHEADTESLLQMLERGALDAALLYDMGLARPVRLEA
                     VTAFVPYALLPAGHALAARSSLPLAALAQEPFILIDLPHSREYFLSLFRQAGVAPRIA
                     HATPSIEMVRSLVANGLGVSLLTTRPVRDWSYDGKRLACRRLRGPLAAQSVVMAYPER
                     LGAANPRAALFARVVRECFTQQRDAMTAPEAP"
     gene            4251..5483
                     /locus_tag="PK518_08570"
     CDS             4251..5483
                     /locus_tag="PK518_08570"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM49009.1"
                     /translation="MSASPPTTRPLDGLRILDFSRVLAGPLSTALLADLGAEVIKVEP
                     PGGDDYRHIGPMRDGISSLFTALNRNKKSLVLDLKHPEAAATVAQLVQNVDVVVENFR
                     PGVAERLGVGPEALRSANPRLVYVSVSGFGQTGPLAHLPAYDIIVQAMSGLMEATGEP
                     EGPPTMVGEAVSDVIAGLFASWATLAALLQQRRTGQGQHVDVAMFDATLAFLVTGVGR
                     YLFTGESARRSGNRHSFSAPFGVYRARDGHFALAVLNNKLFATLAALIGVPGFAQDAR
                     FATDELRRAHEGELAAAIEAWAGQHSVAEVVAQLEAAGVPGSPLWTMAQALESEQGLA
                     RGLLSAVDDPRLPGLRVPTQPVKFGGSAPNRTEPAPALGQHTDELMAGLLQAGGERLA
                     ALRAAGLFGPAMTHTVSL"
     gene            5502..6653
                     /locus_tag="PK518_08575"
     CDS             5502..6653
                     /locus_tag="PK518_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM49010.1"
                     /translation="MNQTNRLGVSPDDQAVADAVARFATETLAPRAREIDEHGLSTTC
                     HVPQLAALGVMGMNLPEQLGGAGVTPTAMLLSLVEISRACAATSSMIGAHYLGTDAVL
                     IGGSPEQHARWLPRAASGEWLAGFALTEPRGGSHPADLRTRAVREGEHYRIDGVKHFI
                     SNAKEARFLVVFAKTDPEAGARGVSAFVVETDSPGISISSPEKLMGIQGAHAFEVAFD
                     GVRVPAANRLGAEGTGFKTAMKVLDNSRLDVAATALGIAEAALADATRWMRERQIGGE
                     PIASKQGLQWMLADMKLRLEASWALTLQALALRQAGQPFTLQSAMAKLHASEMVGFVT
                     DAALQIHGGYGYTREMPLERYVRDARILRIYEGSSEVQRNIIARMVLAD"
     gene            6864..7829
                     /locus_tag="PK518_08580"
     CDS             6864..7829
                     /locus_tag="PK518_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM49011.1"
                     /translation="MSQAVVRMASLVAAVAGVVGLVAPAQAQEAKVTLGMSGWTGFAP
                     LTLADKAGIFKKNGLDVELKMIPQKDRHLALASKAIQCAATTVETHVAWNTNGVPIVQ
                     IFQLDKSYGADGLAVRNEVKSFADLKGKTIGVSAPGTSPYFGLAWMLSKNGMSLKDVK
                     VVSLEPQPAAQAFVSGQNDAAMTYEPYLSTVRANPQAGKILATTIDYPMVMDTVGCDP
                     AWLKANPKAAQALANSYFEAVEMVKADPAKSYEIMGAAVKQTGEAFAKSASYLRWQDK
                     AANQKFFAGELQQFMKDAAAILLEAGVIRKLPDNYGAMYDGSFIK"
     gene            7855..8700
                     /locus_tag="PK518_08585"
     CDS             7855..8700
                     /locus_tag="PK518_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49012.1"
                     /translation="MNQAMSVTPPLGAQEAAPRRRRALAPLEPVSARARVALGVGFFV
                     MFFAVWAFFTLGGFVSPTFLASPITMAREGWLLFTEFGFLHDIGMTVWRVLGGFILAA
                     IVAVPLGVAMGAHKGVEAFLEPFVSFCRYLPASAFIPLLILWAGLGEVQKLLVIFIGS
                     VFQIVLMVAVTVGNARKDLVEAAYTLGAKPSGIVRRVLIPGAAPDIAEILRLVLGWAW
                     TYVIVAELIGSSSGIGHMITDSQALLNTGQIIFGIIIIGLIGLVSDSFFKYINRRMFA
                     WSALR"
     gene            8697..9476
                     /locus_tag="PK518_08590"
     CDS             8697..9476
                     /locus_tag="PK518_08590"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM49013.1"
                     /translation="MSQLSIQGVSRTFTSEKGKSTQALLPVDFDVKENDFVTILGPSG
                     CGKSTMLRIVAGLDHPTSGRVLLDGRPVEGPGADRGMVFQSYTLFPWLTIEQNIRFGL
                     RERGMPESQQKERAQYFIAKVGLRGFEQHFPKQLSGGMQQRTAIARALANDPKILLMD
                     EPFGALDNQTRVLMQELLLGIWEAERKTVMFVTHDIDEAIFMANRVAVFSARPGRIKS
                     EIAVNLPHPRHYTIKTSPEFMDLKARLTEEIRAESMAAEAH"
     gene            9491..10261
                     /gene="hutC"
                     /locus_tag="PK518_08595"
     CDS             9491..10261
                     /gene="hutC"
                     /locus_tag="PK518_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HRM49014.1"
                     /translation="MPTASDFRTKAAQLETPALALYQQVKDYIARKIQDGSWKAGDRL
                     PSENELVLQFGISRMTVNRALRELTEQGRIVRVAGVGSFVAEEKPQSTLLQIAHLASE
                     IRQRGHDYDCRLLSVERVAATMDVATALDMRLGESVFHALCVHLENGVPVQLEDRYVN
                     PRMAPDFLQQDFVQVPPSEYLVRNVPFDQAEHVVEAVLPTAEQARLLEMGAAQPCLLL
                     IRRTWSRGVPVTMVHCLHPASRYRLGSRFRADGNPVLG"
     gene            10316..11875
                     /gene="hutH"
                     /locus_tag="PK518_08600"
     CDS             10316..11875
                     /gene="hutH"
                     /locus_tag="PK518_08600"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRM49015.1"
                     /translation="MTTANQASCDTLVLRPGHVSLAELRRLSAGGVLLTLDPAALAGM
                     LAAQATVQTIVDEDQVVYGINTGFGKLASTKIAHDHLADLQRNLVLSHSVGTGEPLPD
                     PVVRMVLATKAVSLARGHSGVRPALVDALLALANADVLPVIPAKGSVGASGDLAPLAH
                     LACVLIGEGQAKVKGQIVSGREAMAAVGLAPFVLGPKEGLALLNGTQVSTALALAGLF
                     QAESVFAAGLVSGALTLEAIKGSVKPFDARIHEARGQAGQIAVAAAVRALLEGSAIDP
                     SHPHCGRVQDPYSIRCVPQVMGACLDNLTHAARVLRIEANAASDNPLVFTDTGEVISG
                     GNFHAEPVAFAADIIALALAEIGAISERRLSLLLDPGLSGLPPFLIRESGLNSGFMIA
                     QVTAAALAAENQCLAHPSSVTSLPTSANQEDHVSMATYGARRLADMARNTAVIVGIEA
                     MSAAQGMEFDRSLKSSPLIEAQFAAIRQRVAFLEQDRYLAPDIESMSQWALRAVLPAA
                     LLSILPSHISA"
     gene            12018..13139
                     /locus_tag="PK518_08605"
     CDS             12018..13139
                     /locus_tag="PK518_08605"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRM49016.1"
                     /translation="MSINKYSQSLASTRLYEQETSLEFLAERHGLEQSNMIDFSLNVN
                     PFGCSAGAIDAISLSLGQVQLYPDVNLSELRLALSSKHCQSPDRFFFGGGLDDVIKLL
                     LQAWASEGDKVLIHLPTFPRYALEARLRGCQVISVQGEVATQFDAERYERALQKDDIS
                     VAFICTPNNPTGEIIPVEVIEHLAGAYPDTIFVVDEALINPIKEGAVGLVDRFDNVAI
                     LRTFSKFFGLAGMRVGYAIANQKLIDIANIGRPPFNVSLPAAVAAIAALQDENFLRQC
                     SEKFLEESTYFRAQLSSHPSVRVVGCHGNMMLLELASCSSAEVASMLASQGIVVADAT
                     SFDGLQNTATLRVSLKGREQNERLIASLRNIDCETATVR"
     gene            13355..>14645
                     /locus_tag="PK518_08610"
     CDS             13355..>14645
                     /locus_tag="PK518_08610"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRM49017.1"
                     /translation="MNATEKFIPADPRFDASRVIRAPRGSQLHCKNWLAEAAYRMLQN
                     NLDPDVAENPKALVVYGGIGRAARNWECYDQILESLRKLEADESLLIQSGKPVGVFKT
                     HENAPRVLIANSNLVPKWANWEHFNELDRKGLFMYGQMTAGSWIYIGSQGIVQGTFET
                     FVEAGRQHYNNDLTGKWILTAGLGGMGGAQPLAATLAGACSLNIECKQSSIDFRLRTR
                     YVDKQARDIDDALALIKHHTERGEAVSIALLGNAAEILPELARRAKAGGIRPDIVTDQ
                     TSAHDLINGYLPMGWTVEQWNAAAADPGQHPTLRAAAAKSCAVHVQAMLDFQAMGVPT
                     VDYGNNIRQVAFDEGVQRAFDFPGFVPAYIRPMFCEGKGPFRWVALSGDPEDIYKTDA
                     KIKELFPENKHTHRWLDMARERIAFQGLPARICWLGLG"
BASE COUNT         2474 a         4876 c         4859 g         2436 t
ORIGIN      
        1 gctacagcat caccgtgccg ggcgtcgtcc ccaagctctc ggccacgccg ggcactatcc
       61 gcaccagcgc gccgcgcctg ggggacgaca ccgatgccgt gctggccgag gcggggctga
      121 cggccgagca gatcgcgctg ctgcgcagca agggagtgat ccaatgacga ccagcgtgtg
      181 gaacggcgcc gggaggcgca tcgagctgtg tgacgtgggc ctgcgcgacg gcctgcagat
      241 ggaggcgcag ttcgtgccga ccgaggacaa gatcgcgctt gtgaatgcac tgtcccaggc
      301 gggcctgtcc aagatcgagg tgacctcgtt cacctcgccc acggccatcc ccgcgctgcg
      361 cgacgccgag gtggtgatgc gcgagatcga gcgccgcccc ggcgtgacct acaccgcgct
      421 cgtgcccaac gcgcgcggcg cggaacgcgc catcgagtcg cgcaccgacg agctgaacct
      481 ggtcatgtcc gtcagcgaga cgcacaacct cgccaacctg cgcatgacgc gcgcgcagtc
      541 gttcgcggca ctggcacagg tgatcaccat ggcgcaggcg gcgcaggtgc cggtgaacgt
      601 gtcgctgtcg tgcgtgttcg gttgccccat ggagggggac gtggccctgg atgccgtatg
      661 ggaccatgtg cagcgcttcg ccgacctggg cgttgcgggc atcacgctgt gcgacaccac
      721 gggcatggcc tttcccacgc aggtggggca ggtggtggcc gctgcgcgcg cgcgctggcc
      781 gggcatggcc ttcacgctgc actttcacaa cacgcgcggc atgggcctgg cgaatgtgct
      841 ggcggccatc gatgcggggg cgcagcgctt tgacgcatcg ctgggcggcc tgggtggctg
      901 tccctatgcg ccgggtgcta cgggcaatgt atgcaccgaa gaagtggtgc atgcgctgca
      961 gctcatgggt tacgacacgg gcattgatct gccggcgctg attgccactg cggcccgcct
     1021 gcccgcgctg atcggccacg aggtgcccag ccagatcgtg aaggcggggt gccggctgga
     1081 cctgcacccg gtgccggccg gcttcgacgc aatccgcgag cgtgcgctgg cgcggtagct
     1141 ttcaccgcac atccataacg acaggagaca accccatgcc tttgaccact gcatctcacg
     1201 gccgcagggc cgcgctggcc tttggggccg ctgtgggcgc actgctgtgc accgcacccc
     1261 tgggcgccgc cgcgcaggac aagtacccct ccaaacccat caccctggtg gtgccgcagg
     1321 ccgcgggcgg cgccaacgac gcgattgcgc gcgtgctggc gcagcgcctg agcgagcaga
     1381 tgggccagag cgtggtggtg gacaaccgcc ccggcgcggg cggcacgctg gccacggcgg
     1441 ccctggcacg tgccaagcac gatggctaca cgctgctggt cacggccgac agcgcgcacg
     1501 tgatcggccc ggcgctctac aagaacccgg ggttcgacgc cgtcaaggat ttcgagcccg
     1561 tggcgcccgt cgccaccgcc ggctatgtgc tggtggcaca tccttcgttc ccgggcaaca
     1621 acgtggcgga cctgatccgg ctggccaagg ccagccccgg caagtacgcc atcgcctcgg
     1681 ctggcaacgg cacgctcaac cacctgatcg gtgagctgct gcaaaaggcc gcgggcatcc
     1741 agctgcagca cattccctac aagggctcgg cggcggccgc caccgacgtg gcgggcgggc
     1801 aggtgccgct gtcggtgcag agcctgccct cgtcgatcgc cttcatcaag gcgggcaagc
     1861 tcaaggtgct gggcgtggtg aacgaaaagc gcgtcgccgc gctgcccgac gtgcccacca
     1921 tcggcgagac gctcaagggc ttcggccagg cgccgtggta cgcgatgttc gcgccggccg
     1981 gcacgccggc accgatcgtc gagcggctgc aggccgaggt ggcgcgcgcg ctggaccaca
     2041 aggacgtggt ggacaagctc gccgtcgtcg gctgcgagcc gttcaagggc tcgtccgccc
     2101 agctcggcgc gctcgtgcag tccgacctgc ccaagtgggc gcgcgtggtc aaggacaccg
     2161 gcgccaccgt ggactgacac cccttctttt caccgacttc cacaactctc acaaggagac
     2221 acaacccatg atgaccacgc gcatcacccg caaacatttc ggcgcgctgg cgctggccgc
     2281 gctggcgccc ctggcgctgt ccacggcctg ggcgcagggt ggctacccgt cgcgcacggt
     2341 gaccttcgtc gtgccggccg ccgccggcgg caccaccgac atctcggcgc gcatggccgc
     2401 gcaggcgctg gccccggtgc tgggccagtc ggtggtggtg gacaacaagg gcggcggcaa
     2461 cggcgccatc gcggccaacc tcgtcaagcg cgccgagccc gacggctaca cgctgctcat
     2521 gcagtactcg ggctatcacg tgatctcgcc ccacctgacc cgcgcgccgc agtgggagca
     2581 gaaggacttc cagcccatcg ccaacgtgat ctcggcgccg cagatcatcg tggtgcgcga
     2641 gagcctgccg gtgaagaccc tggccgagct ggtggcctac gccaaggcca accccggcaa
     2701 gctcaactac gcctcgtcgg gcaacggctc gctgcagcac gtgacgggtg ccatgctgga
     2761 acagcagggc ggcatcaaga tggtgcacgt gccctacaag ggcacgggcc cggcgctgca
     2821 ggacctgctg ggcggccagg tggatctgac cttcggcacg gcgccgccgt tcatgcccca
     2881 catccagtcc ggcaagttgc gcgtgctggc cgtgaccggc aaggaacgcc tggccagcct
     2941 gcccgacgtg cccaccacgg ccgaggccgg ctacgccggc gtgaacgcca cctcgtggtt
     3001 cgcgctgttc gcgcccgcgg gcgtgcccaa gccggtgatc gacaagctgg ccgccgacat
     3061 gaagaaggtg gtggaggacc ccgccttccg caagaaggcc caggaacagg gcgccgcggc
     3121 cgactaccag agcccgcaac agctgggcga gcgcgtgagg agcgacttcg ccaactgggc
     3181 gggcgtgatc aagagctcga agatcgaggc cgagtaaggc gccgcgcagg tgtcaaggcg
     3241 cctccggcgc cgtcatggcg tcgcgttgct gcgtgaaaca ctcgcgcacg acacgcgcga
     3301 acagcgcggc gcgcggattg gctgcaccca ggcgctcggg gtaggccatg accaccgact
     3361 gggcggccag cggtccgcgc aggcgccggc aggccaggcg cttgccgtcg taactccagt
     3421 cgcgcaccgg gcgcgtggtg agcagggaga cgcccaggcc attggccacg agcgagcgca
     3481 ccatttcgat cgagggtgtg gcatgggcga tgcgaggtgc cacgcccgcc tggcggaaca
     3541 gcgagaggaa gtactcccgg ctgtgcggca ggtcgatgag gatgaagggc tcctgggcca
     3601 gcgcggccag cggcaggctg ctgcgtgctg cgagcgcgtg gcccgcgggc agcagggcgt
     3661 agggcacgaa ggccgttacg gcctccaggc gcaccggacg tgccaggccc atgtcataga
     3721 gcagggcggc atcgagggcg ccgcgctcaa gcatctgcag cagcgactcg gtgtcagcct
     3781 cgtgcacctc gatctccacc cgcggatggc gctcgcgcat gcgcagcagc atggcgggca
     3841 ggtggcgtgg tgccagcgtg gccaggcagc cgacgcgcag cagtccggct tcctcgtccg
     3901 tgcgcggccc ttcgagcgcg cgtgcgcgct caagcagcgc gtgcgcctcc cggtggcgca
     3961 tctggcccgc ggccgtaagc tccaggccgc gcgcatggcg gcgcacgaac agcgtttcgc
     4021 cccacagcgc ctccaggtcg gcaatggcct tggagatgct gggctgcgac acgctgagcg
     4081 cctcggccgc gcgcgccgcg ccgccatgct gggccgcggc ggcgaagtat tcgagctggc
     4141 gcaggctgat gtttatcaaa atgattgatt caaattatca agattgaata ttttattctg
     4201 atatcggttt gggcaaccat gggcaccttg catacccgaa ggagccccgc atgtccgcat
     4261 cgccccccac caccaggccg ctcgacggct tgcgcatcct tgatttcagc cgcgtcctcg
     4321 cggggccgct gtccacggcg ctgcttgccg acctgggggc cgaggtcatc aaggtcgagc
     4381 cgcctggcgg tgacgactac cgccatatcg gccccatgcg cgatggcatc agcagcctgt
     4441 tcaccgcgct caaccgcaac aagaagagcc tggtgctgga cctcaagcac cccgaagccg
     4501 cggcaaccgt cgcccaactg gtccagaacg tggatgtggt ggtggaaaac ttccgccccg
     4561 gcgtggccga gcgcctgggc gtgggccccg aggcgctacg cagtgccaac ccacgcctgg
     4621 tgtacgtgag cgtgtcgggc ttcggccaga cggggccgct ggcgcacctg ccggcctacg
     4681 acatcatcgt gcaggccatg agcggcctga tggaggccac gggcgagccc gaggggccgc
     4741 ccaccatggt gggcgaggcc gtgtcggacg tgatcgccgg cctgttcgcg tcctgggcca
     4801 cgctggccgc gctcttgcag cagcgccgca cgggccaggg ccagcatgtg gacgtggcca
     4861 tgttcgacgc cacgctggcc ttcctcgtca caggcgtggg ccgctacctg ttcacgggcg
     4921 agagcgcgcg gcgcagcggc aatcggcatt ccttctcggc accgtttggc gtgtatcgcg
     4981 cgcgcgacgg gcattttgcg ctggccgtgc tcaacaacaa gctgtttgcc acgctggcgg
     5041 cgttgattgg cgtgcctggt ttcgcgcagg acgcgcgctt tgccaccgac gagctgcgcc
     5101 gggcgcacga gggcgagctg gccgccgcca tcgaggcctg ggccggccag cacagcgtgg
     5161 ccgaggtggt ggcgcagctg gaggccgcgg gcgtgcccgg cagcccgttg tggaccatgg
     5221 cgcaggccct cgaatccgag cagggcctgg cgcgcggcct gctgtccgcc gtggacgatc
     5281 cgcgcctgcc cgggctgcgc gtgcccacgc agcccgtgaa gtttggcggc tctgccccga
     5341 accgcaccga gcccgcgcct gcgctgggcc agcacaccga cgagcttatg gccgggctgc
     5401 tgcaggcagg cggcgagcgg ctggccgcgc tgcgcgcggc gggactgttc ggccctgcca
     5461 tgacacacac cgtttcactt tgatgttcag gagccgatga aatgaaccag acaaaccgct
     5521 tgggcgtgag ccccgatgat caggccgtgg ctgacgcagt ggcccgcttc gccaccgaga
     5581 cccttgcgcc gcgcgcgcgg gagatcgatg agcacggcct gtccactacc tgccatgtgc
     5641 cgcaactggc cgcattgggc gtgatgggca tgaacctgcc agagcaactg ggtggcgcgg
     5701 gtgtcacgcc cacggccatg ctgctgtcgc tggtggaaat ctcgcgcgcc tgcgctgcca
     5761 cctcctcgat gatcggcgcg cattacctgg gtaccgatgc ggtgctcatc ggcggcagcc
     5821 ctgagcagca cgcacgctgg ctgccgcgcg cggccagcgg cgagtggctg gcgggcttcg
     5881 cgttgaccga gccgcgcggc ggctcgcatc cggccgacct gcgcacgcgc gccgtgcgtg
     5941 aaggcgagca ttaccgcatc gacggcgtga aacatttcat ctcgaatgcg aaggaggcca
     6001 ggtttctcgt ggtctttgcc aagaccgacc cggaagcggg tgcgcgcggc gtcagcgcct
     6061 tcgtggtgga gactgactct ccgggcatca gcatttcatc gcccgagaag ctcatgggca
     6121 tccagggggc gcacgcgttc gaggtggcgt tcgacggcgt gcgcgtgccc gccgcgaacc
     6181 gcctgggcgc cgagggcacg ggcttcaaga cggcgatgaa ggtgctggac aacagccgcc
     6241 tggacgtggc ggccacggcc ctgggcatcg ccgaggccgc gctggccgac gccacgcgct
     6301 ggatgcgcga gcgccagatc ggcggcgagc ccatcgccag caagcagggc ctgcaatgga
     6361 tgctggcgga catgaagctg cgcctggaag catcctgggc cctcacactg caggcgctcg
     6421 cgctgcgcca ggcaggccag ccattcacgc tgcagtcggc catggccaag ctgcacgcct
     6481 ccgagatggt gggctttgtg accgacgccg cgctgcagat ccacggcggc tacggctaca
     6541 cgcgcgagat gccgctggag cgctacgtgc gcgatgcgcg catcttgcgc atctacgagg
     6601 gctcgtccga ggtgcagcgc aacatcatcg cacgcatggt gctggccgac tgatcggccc
     6661 ttgcggtgcc ccgttgcggg aagcgccgca gcgtgttccc gggaggtggg aagaaggccg
     6721 ttttcctgac gtccgagcag tgtattcctg gtgtttaccc tgtatagaca ggtgcgaggg
     6781 agccccgatc atcgcggcaa ggaagcaatg cctgcttccc aatctgggca agccaaagaa
     6841 ctttttccta caggagacga tatatgagcc aagcggttgt gaggatggcg tcgctggtgg
     6901 cggcggtggc aggcgtggtg gggttggtcg caccggcgca ggcccaggag gccaaggtga
     6961 cgctgggtat gtcgggctgg acaggttttg caccgttgac gctggccgac aaggccggca
     7021 tcttcaaaaa gaacggcctg gatgtggagc tgaagatgat tccgcagaag gaccgccacc
     7081 tggccctggc ctccaaggcc atccagtgcg ccgcgaccac ggtggagacg catgtggcct
     7141 ggaacaccaa tggtgtgccc atcgtgcaaa tcttccagtt ggacaagtcg tatggcgccg
     7201 atggcctggc ggtgcgcaat gaggtcaaga gctttgccga cctcaagggc aagaccatcg
     7261 gtgtgagtgc cccgggtacg tcgccgtact tcggcctggc ctggatgctg tccaagaacg
     7321 gcatgagcct caaggacgtc aaggtcgtct cgctggagcc ccagcccgcg gcccaggcct
     7381 tcgtgtcggg ccagaacgat gcggccatga cctatgagcc ctacctgtcc accgtgcgcg
     7441 ccaacccgca ggccggcaag attctggcca cgacgatcga ctatcccatg gtgatggaca
     7501 ccgtgggttg cgaccccgcc tggctcaagg cgaaccccaa ggccgcgcag gccctggcca
     7561 actcgtactt cgaggcggtg gagatggtca aagccgaccc cgccaagtcc tacgagatca
     7621 tgggcgcggc cgtcaagcag acgggcgagg cctttgccaa gtccgcgtcc tacctgcgct
     7681 ggcaggacaa ggcggcgaac cagaagttct ttgcgggcga actgcagcaa ttcatgaagg
     7741 atgccgcggc catcctgctc gaggccggcg tgatccgcaa actgccggac aactacggcg
     7801 cgatgtacga cggcagcttc atcaagtgag cacatggtgc caaaggacag cgatatgaat
     7861 caagcaatga gcgtaacgcc cccgctcggg gcgcaggagg cggcgccgcg ccgccgccgt
     7921 gccctggcac cgctggagcc cgtgagcgcc cgcgcgcggg tggccctggg ggtgggattt
     7981 tttgtgatgt tctttgcggt gtgggccttc ttcacgctgg gcggctttgt ctcgcccacg
     8041 ttcctggcca gccccatcac catggcccgg gaaggctggc tgctgttcac cgagttcggc
     8101 tttctgcacg acatcggcat gaccgtgtgg cgcgtgctgg gcggcttcat cctggcggcc
     8161 atcgtggccg taccgctggg cgtcgccatg ggcgcgcaca agggagtcga ggcgtttctg
     8221 gagcccttcg tgtcgttttg ccgctacttg ccggcctcgg ccttcattcc gctgctaatt
     8281 ctgtgggcgg ggctgggcga ggtgcaaaag ctgctggtga ttttcatcgg ttcggtgttt
     8341 cagatcgttc tgatggtggc ggtcaccgtg ggcaatgcgc gcaaagacct ggtggaagcc
     8401 gcctacacac tgggcgccaa gccctcgggt atcgtgcgcc gcgtgctgat cccgggcgcc
     8461 gcgcccgaca ttgccgaaat cctgcgcctg gtgctgggct gggcctggac ctatgtcatc
     8521 gtggccgagc tgattggctc atcctcgggt ataggccaca tgatcaccga cagccaggct
     8581 ttgttgaata cgggccagat catcttcggc atcatcatca tcggcctcat cggtctggtg
     8641 tcggattcgt tcttcaaata catcaaccgc cgcatgtttg catggagtgc cctgcgatga
     8701 gccagctatc gatccaaggt gtttcgcgca cattcaccag cgaaaaagga aaatccacgc
     8761 aggctctgct gccggtcgat ttcgacgtca aggaaaacga ctttgtcacc atccttggcc
     8821 cttcgggctg cggcaagtcc acgatgctgc gcatcgtggc ggggctggat catccgacct
     8881 cgggccgcgt gctgctcgat ggccgtccgg tggagggccc cggcgccgat cgcggcatgg
     8941 tgttccagag ctacacgctg tttccttggc tcacgatcga gcagaacatc cgtttcggcc
     9001 tgcgcgagcg cggcatgccc gaatcgcagc agaaggagcg cgcgcagtat ttcatcgcca
     9061 aggtggggct gcgcggcttc gagcagcatt ttcccaagca gctttcgggc ggcatgcagc
     9121 agcgcacggc gattgcccgg gccctggcca atgaccctaa gattttgctc atggacgagc
     9181 ccttcggtgc gctcgacaac cagacccgcg tactcatgca ggagttgctc ttgggtatct
     9241 gggaggccga gcgcaagacc gtgatgttcg tcacgcacga catcgacgag gccatcttca
     9301 tggccaaccg cgtggccgtg ttcagtgcgc gcccggggcg catcaagagc gagatcgccg
     9361 tgaatctgcc gcacccgcgc cattacacga tcaagacttc gcccgagttc atggatctga
     9421 aggcgcgcct caccgaggaa atccgcgccg agtccatggc cgccgaggcg cattgagggc
     9481 gcgcagcacc atgccgacag catccgactt ccgcaccaag gctgcgcaac tcgagactcc
     9541 ggctctggcc ctgtaccagc aagtcaagga ctatattgcg cgcaagatcc aggacggctc
     9601 gtggaaggcg ggcgaccgcc tgccctccga gaacgaactg gtgctgcagt tcggcatctc
     9661 gcgcatgacc gtgaaccgcg ccctgcgcga gctcacggag caggggcgca tcgtgcgcgt
     9721 ggcgggcgtg ggcagcttcg tggccgagga aaagccccag tccacgctgc tgcagatcgc
     9781 ccatttggcg agtgaaatcc gccagcgcgg gcacgactac gactgccgac tgctcagcgt
     9841 ggagcgcgtc gccgccacca tggacgtggc gacggccctg gacatgcgcc tgggcgaatc
     9901 ggtgttccac gccctgtgcg tgcacctgga aaacggcgtg ccggtgcagc tggaagaccg
     9961 ctatgtcaac ccgcgcatgg cgcccgattt cttgcagcag gatttcgtcc aggtgccgcc
    10021 ctccgagtat ttggtgcgca acgtgccctt cgaccaggcc gagcatgtgg ttgaggccgt
    10081 gctgcccacg gccgagcagg cacgcctgct ggagatgggc gcggcccagc cctgcctgct
    10141 gctcatccgg cgcacctgga gccggggcgt gcccgtgacc atggtgcact gcctgcatcc
    10201 cgcatcgcgc tatcgcctgg gcagccgctt ccgtgcagac ggaaacccgg tgcttggata
    10261 agacttcaca ccttcttttt cgcttgtgcc aacttgtata tacaggatta aacttatgac
    10321 tactgcaaat caagcctctt gtgacactct ggtgctgcgg cctggacatg tcagcctggc
    10381 cgagctgcgc cgcctgagcg cgggtggcgt acttctcacg ctcgatcccg cagcgttagc
    10441 gggcatgctg gcggcgcagg ccacggtgca gaccatcgtc gatgaagacc aggtggtcta
    10501 tggcatcaac accggctttg gcaagctggc cagcacaaag atcgcccacg atcacctggc
    10561 cgacctgcag cgcaacctgg tgctgtcgca cagcgtgggc acgggcgaac cgctgcccga
    10621 cccggtggtg cgcatggtgc ttgccaccaa ggccgtaagc ctggcgcgcg gccactcggg
    10681 cgtgcgcccg gcactggtcg atgcgctgtt ggccctggcc aatgccgatg tgctgccggt
    10741 gatcccggcc aagggctcgg tgggggcctc gggcgacctc gcgccgctgg cgcacctggc
    10801 ctgcgtgctg atcggcgagg gacaggccaa ggtcaagggc cagatcgtct caggccgcga
    10861 ggcgatggcg gccgtggggc tggcgccctt cgtgctgggc cccaaggagg ggctggcgct
    10921 gctcaatggc acgcaggtgt ccacggcgct ggcgctggcc ggcctgttcc aggccgagag
    10981 cgtgttcgcg gccggcctgg tgtcgggcgc gctgacgctc gaggccatca agggttcggt
    11041 caagccgttc gacgcgcgca ttcacgaggc gcgtggccag gcgggccaga ttgccgtggc
    11101 ggccgccgtg cgcgcgctgc tcgaaggcag tgccatcgac ccctcgcacc cgcactgcgg
    11161 ccgcgtgcag gacccgtact ccatccgctg cgtgccccag gtcatgggcg cctgcctgga
    11221 caacctcacg cacgccgcgc gcgtgctgcg catcgaggcc aatgccgcgt cggacaaccc
    11281 gctggtgttc accgataccg gtgaggtgat ctcgggcggc aacttccacg ccgagcccgt
    11341 ggccttcgcg gccgacatca tcgcgctggc gctggccgag atcggcgcca tctccgagcg
    11401 gcgtctgtcg ctgctgctcg accccggcct gtcgggcctg ccgccgttcc tgatccgcga
    11461 gagcggtctg aactcgggct tcatgatcgc ccaggtcacg gccgcggccc tggccgctga
    11521 gaaccagtgc ctggcccacc ccagcagcgt gaccagcctg cccacctcgg ccaaccagga
    11581 ggaccatgtc tccatggcca cctacggcgc gcggcgcctc gcggacatgg cgcgcaacac
    11641 ggccgtgatc gtgggcatcg aggccatgtc ggccgcgcag ggcatggagt tcgatcgcag
    11701 cctgaagtct tcaccgctca tcgaggcgca gttcgccgcc attcgccaac gggtggcctt
    11761 cctcgaacaa gaccgctacc tcgcacccga tatcgagagc atgagccagt gggccttgcg
    11821 cgcggtactg cctgctgccc tgctgagcat cctgcccagc cacatttcag cttaaggaga
    11881 cttgtcatga cccaacaaga cacccgcttc aacgccagcc gtgaaataca tgttccccac
    11941 ggcagtacat ctgggcagca acaagaaccc attcagtgcc agccccgctt tgcccggtca
    12001 ttctgagcaa acttgatatg agcatcaaca aatacagcca gtctctggca agcacccgac
    12061 tctacgaaca agagacctcc ctcgaattct tggcggagag acacggcctc gagcaatcga
    12121 acatgatcga tttctccctg aacgtaaatc cgtttggctg ctccgcaggc gcaatcgatg
    12181 caattagcct gtcacttggg caagtgcagc tttatcctga tgtcaatctt tccgagctgc
    12241 gactcgcgct ctcgagcaag cattgccaat caccggatcg gttcttcttc ggcggggggc
    12301 tcgacgatgt catcaagctt ttattacagg cctgggcctc agagggggac aaggttctca
    12361 tccacctgcc cacgtttccc cgctacgcgc ttgaggcgcg cctacgggga tgccaggtaa
    12421 ttagcgtaca gggcgaagtg gcaacgcagt tcgatgccga gcgctacgaa cgtgcattgc
    12481 agaaagatga catctctgtc gcctttatct gcacgccgaa caatccgact ggagagatca
    12541 tcccggtgga ggtgatcgag catctggccg gggcctaccc ggacacgatc ttcgtggtcg
    12601 acgaagcact tatcaatccc atcaaagagg gggctgtcgg actggtcgac aggtttgaca
    12661 atgtcgccat tttgcgaacc ttctcgaagt tttttgggct cgctggcatg cgcgtgggct
    12721 atgccattgc taaccaaaag ctcattgata tcgccaacat cggacgccca ccgttcaatg
    12781 tttcgcttcc tgcggcggtt gcagccatcg ccgcccttca ggatgagaat tttctacgtc
    12841 aatgcagcga gaaatttttg gaggaaagca cctacttccg tgcgcaactc agtagccatc
    12901 cgtcggttcg cgttgtcggt tgccatggaa acatgatgct gctagagctt gcctcttgct
    12961 cctccgccga agttgcatcg atgctcgcga gtcagggcat tgttgtggca gatgcgacat
    13021 ccttcgacgg ccttcagaac acggccacgc tgagggtgtc gcttaaagga cgcgagcaga
    13081 acgaaaggct tatcgcttcc ctgcgcaata tcgactgtga aactgcgaca gttcgttaat
    13141 caagcacaac ctgaaaagcc caccgctgtt agaggccgaa tttggcaagc tgcgcgaaaa
    13201 ggcgtccttc ctggacgccg acctctattt ggcgcccgat atcgaggccg tgcgccagtg
    13261 ggcgctgagg gccgatcttc ccgccacgct gctgagcatc cggcccagcc ccgtctgaac
    13321 cctgtcattg ttttttttca ctggagtatt tgccatgaac gccaccgaaa aattcattcc
    13381 cgccgatccc cgctttgacg ccagccgcgt gatccgcgcg ccgcgcggca gccagctgca
    13441 ctgcaagaac tggctcgccg aagccgccta ccgcatgctc cagaacaacc tggatccgga
    13501 cgtggccgag aaccccaagg ccctggtggt ctacggcggc atcggccgcg cggcgcgcaa
    13561 ctgggagtgc tacgaccaga tcctggagtc gctcaggaag ctcgaagccg atgagtcgct
    13621 gctgatccag tccggcaagc ccgtgggcgt attcaagacc cacgaaaacg caccgcgcgt
    13681 gctgatcgcc aactccaacc tggtgcccaa gtgggccaac tgggagcatt tcaacgagct
    13741 cgaccgcaag ggcctgttca tgtacggcca gatgaccgcc ggcagctgga tctacatcgg
    13801 cagccagggc atcgtgcagg gcaccttcga gaccttcgtc gaggccggcc gccagcatta
    13861 caacaacgac ctgacgggca agtggatcct cacggccggt ctgggcggca tgggcggcgc
    13921 ccagcctctg gccgccacgc tggccggcgc ctgctcgttg aacatcgagt gcaagcagtc
    13981 cagcatcgac ttccgcctgc gcacgcgcta tgtggacaag caggccaggg acattgacga
    14041 tgcgctggcc ctgatcaaac accacaccga aaggggcgag gccgtctcca tcgccctgct
    14101 gggcaatgcc gcggaaatcc tgcccgagct ggcccgccgc gccaaggctg gcggcatccg
    14161 gcccgacatc gtgaccgacc agacctcggc ccacgatctg atcaacggct acctgcccat
    14221 gggctggacc gtggagcagt ggaacgccgc cgcggccgat cccggccagc atcccacgct
    14281 cagggccgcg gccgccaaga gctgcgccgt gcatgtgcag gccatgctcg acttccaggc
    14341 catgggcgtg cccacggtgg actatggcaa caacatccgc caggtggcgt tcgacgaggg
    14401 cgttcagcgc gccttcgact tccccggttt cgtgcccgcc tacatccggc ccatgttctg
    14461 cgagggcaag gggccgttcc gctgggtggc gctgtcgggc gatcccgagg acatctacaa
    14521 gaccgatgcc aagatcaaag agctgttccc cgagaacaag cacacgcacc gctggctgga
    14581 catggccagg gagcgcatcg ccttccaggg cctgcccgcg cgcatctgct ggctgggcct
    14641 gggcg
//
LOCUS       DAOMTQ010000072        14587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_669_length_14587_cov_23.274566, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000072 DAOMTQ010000000
VERSION     DAOMTQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14587
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_669_length_14587_cov_23.274566"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK518_08615"
     CDS             complement(<1..237)
                     /locus_tag="PK518_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49018.1"
                     /translation="MAVLLAALLAACSNQPPVPDWQQNAHGAAERASAAYLAGNDRVA
                     AQEWQRARAELARTGRPDALAQLELLRCAAQATSL"
     gene            complement(450..1433)
                     /locus_tag="PK518_08620"
     CDS             complement(450..1433)
                     /locus_tag="PK518_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRM49019.1"
                     /translation="MPETLPPPPPEELLRPVAHLRLKAAALLLLTLALIAGSVLYLLY
                     ARGAFEPTQRLVLIADDSEGVSVGMDMTFSGFPIGRVRRIELAGDGNVRIAVDVASRD
                     AHWLRESSVFTLVRGLVGGTTIKAYSGVLTDPPLAAGAERPVLRGDATAEIPQLMATA
                     RQLLDNLQQLSAPDSALNDTLAQVRQLTQRLNAPGGALGVLMGNEQDARKVLQTLDRT
                     QQLLARMDAVVARADRQVFGAAGQPGLVAELRATVAQASGLLQDTRQSLTRVDAVLAE
                     AQAVGANAREASADLGALRAEVEANLRKLEGLINDINRKWPFARDQELRLP"
     gene            complement(1448..2242)
                     /locus_tag="PK518_08625"
     CDS             complement(1448..2242)
                     /locus_tag="PK518_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49020.1"
                     /translation="MFDASLDRLWRGAQRWALGWWRIIDLGAVVLVLLLSPSSYGRGA
                     RDRLARQIYTDTLPILPGFTALAALLSLVITHIVVVTALSYGLTRYALEMVIRVLVLE
                     LIPLTAALFVAMRTTIPSGAQLARLRQAGVLQAWRARGADPLRVELLPRVLAGAYASV
                     TLAALSCVVALVMAYLGVYGLTTAGLSAYTRMFGQVFTPSITLVFTLKTLLFSLAVAL
                     IPMAGGLHPPPPGALLGQDGAAPASLARLFAVLLLIEALSLMGNYY"
     gene            complement(2235..2696)
                     /locus_tag="PK518_08630"
     CDS             complement(2235..2696)
                     /locus_tag="PK518_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM49021.1"
                     /translation="MPQQSPQHRIGDAARLSGVSAANIRYYEKEGLLRPGVRGDNSYR
                     FYSSADLHRLRFIRLCRAMDMSLDEVRRLLAIDAASDQADHQACATLDEHLAHVRQRL
                     AELRVLEKELRGLRGRCDGQADHCQVIEALHERAEAQDLPPQPSASALRHV"
     gene            2736..4760
                     /locus_tag="PK518_08635"
     CDS             2736..4760
                     /locus_tag="PK518_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM49022.1"
                     /translation="MNHSTSPLGHDHGHAHDRPHTHEHGAANTACSACSSCATDDDHD
                     HGTLPPWPRIAAALVLALGAEAAHWLQWTWPGMALAVAAIALSGLGVYRAGLKDLARL
                     RLGINALMTVAVTGAVLIGQWPEAAMVMALYVAAERLEHGAMDRARNAIRDLLKLAPA
                     TADVLQADGSVQRLPVAQVPLGATLRIAPGAHVPLDGQVTQGSSAVNQAPITGESQLA
                     DKAPGDELYAGSVNQHGELLMRVSAAPADTLLARIVRAVEQAQASKAPAQRFVDRFAA
                     VYTPIVFVLAVLLALAAPQFMGWSWQQAAYQALALLVIACPCALVISTPVTVVSALTA
                     AARRGILIKGGNALEAARGLRAVALDKTGTLTTGNPALVQWQALAGFDGAQAAAIAWQ
                     LASRSEHPVSRAIATGLPAVAHNGVTQLQALPGRGVQADIDGQRWQLGNLRLMREQGL
                     ADAALLATLEEHERQGRSITLLAGGGAVHAWFAVADPLRAHVPQAIAQLRALGVQPMV
                     LSGDNPATVQAIALEAGISAEDARGGLLPEDKLAALAELQHRHGPTAMTGDGINDAPA
                     LARADLGFAMGGAHSTAMAMETAGVVLMHDDLRRIPDTLRLAQKTHRVLWQNISLALG
                     VKAVFFMLALLGLATMWLAVLADMGVSLLVVANGLRLRHALPATSDQPRP"
     gene            4757..5140
                     /locus_tag="PK518_08640"
     CDS             4757..5140
                     /locus_tag="PK518_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HRM49023.1"
                     /translation="MSQTRQRAAFDYLLQQFAASAGQSAAQRWRYLEAAHVLGQNRLA
                     LHLRAHWQMLRFAQALGDQHEARGQLSHLCMAPVGHIVRRMPYGNNGRAIVPALHPMV
                     PSAALRSCISEAMQAVGGDVPQDPA"
     gene            5206..5826
                     /locus_tag="PK518_08645"
     CDS             5206..5826
                     /locus_tag="PK518_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM49024.1"
                     /translation="MSATITTPAPAPHTRVEDLVAIVTGVAMVSVGVALVGSAGLLTG
                     GITGLAFVLHYATGIGFGKLFFVLNLPFYWLALRKLGLGFTVKTFAGVALLAVLTEVQ
                     PLLMQVGSIQPLYAALVGGLCMGMGFLVLFRHHCSLGGIGILALYLQGRFGWRAGAVQ
                     MAADFCIVAAALVTVEPMRVLWSVIGAVALNQVLAINHRPGRYMAA"
     gene            5861..6226
                     /locus_tag="PK518_08650"
     CDS             5861..6226
                     /locus_tag="PK518_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49025.1"
                     /translation="MRRVLSFALVMLLLMRGLPGDAMALGQAPVQPTPQMAQMAHAMD
                     ADAHHAAAMAQDCCDHASDSHMAHQGGCSACGVCHSTLAVSPWMAVPGTGAQHSPQAP
                     PGTRFTSALAAQAIKPPIF"
     assembly_gap    6315..6414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6415..6805
                     /locus_tag="PK518_08655"
     CDS             <6415..6805
                     /locus_tag="PK518_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HRM49026.1"
                     /translation="AAPQQQAWGIAGQAGKATRTITLRMTDDMRFTPSHFSVKQGETV
                     RLRVENKGQVLHEVVLGTQASLDEHAAMMRQHPDMEHGEAHMAHVKPGRKDDLVWNFN
                     RTGSFDFACLVAGHFEAGMRGSFTVTP"
     gene            6831..7313
                     /locus_tag="PK518_08660"
     CDS             6831..7313
                     /locus_tag="PK518_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HRM49027.1"
                     /translation="MHNTTRRQWLTAIAAASACAALPAWATSNANAVEVWKDPSCGCC
                     QDWIDHMQANGFKVTVHETGNAAVRAKLGLPQRLGSCHTALVGGYLLEGHVPAADVHK
                     LLKEKPKALGLTVPGMPVGSPGMDGPEYKGRKDAYDVLLVTKNLMNKDVSTRVFTSYR
                     "
     gene            7378..8385
                     /locus_tag="PK518_08665"
     CDS             7378..8385
                     /locus_tag="PK518_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRM49028.1"
                     /translation="MHDPSDPGAITHVAGIEVGHHSDSRRPTGCTVVMARAGAVAGVD
                     VRGAAPGTRETDLLAPGNLVDKVHAVLLAGGSAWGLDAATGAVRWLEEQGVGLDVGVG
                     RLPLVPAAVLFDLHVGDMKIRPDAAAGYAACAAASRQAPEEGNVGAGSGAVVGKMFGM
                     QHAMKGGLGTASVTVAGVTVGALIACNAVGDVVDPATGLPLAGARTADGQALRDTRRA
                     LLAGAPPHPLLAGSNTTIGVIATDAPLSKVQAQRLAVAGHDGLARAINPVHTMSDGDT
                     LFTLATGVREQHPGMLVLATMAAEAVARATVRAVLTARALRLNDGLRLPCHADMVSTG
                     G"
     gene            complement(8461..10122)
                     /gene="ettA"
                     /locus_tag="PK518_08670"
     CDS             complement(8461..10122)
                     /gene="ettA"
                     /locus_tag="PK518_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRM49029.1"
                     /translation="MAQYVFSMNRVTKTVPPKRQILKDISLSFFPGAKIGVLGLNGSG
                     KSSLLKIMAGIDKEFEGEAIPMQGLSIGYLPQEPQLNPEHTVREAVEEAMAEVNNAKA
                     RLEEVYAAYAEEDADFDALAAEQGKLEAIIAAAGTDSEHQLEIAADALRLPPWEAVIG
                     QLSGGEKRRVALCQLLLSKPDMLLLDEPTNHLDAESVEWLEQFLHRFTGTVVAITHDR
                     YFLDNAAEWILELDRGHGIPYKGNYSDWLLQKQNRLEAEQKGEEARAKALKKELEWVR
                     QNAKGRQAKSKARIARFEELSDYEYQRRNETQEIFIPVAERLGSKVIEFNNVSKAFGD
                     RLLIDNLSMNIPAGAIVGIIGPNGAGKSTLFKLIAGKEQPDSGGVEIGQTVKMAFVDQ
                     HRDALANEKTVWEDVSGGLDIINVGKFQMASRAYCGRFNFNGQDQQKKVGNLSGGERG
                     RLHLAKTLIQGGNVLLLDEPSNDLDVETLRALEDALLEYAGTVLVISHDRWFLDRIAT
                     HILAAEGDSQWVFFDGNYQEYEADKKRRLGEEGAAPKRMRYKALK"
     gene            10381..12099
                     /locus_tag="PK518_08675"
     CDS             10381..12099
                     /locus_tag="PK518_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM49030.1"
                     /translation="MTFEELKLAPAILQAVHEQGYNNPTPIQAQAIPAVLAGHDLLAG
                     AQTGTGKTAAFTLPMLHRLAGSAAATSKFGNRGVRALVLTPTRELAAQVEESVRTYGK
                     HLALKSTVIFGGVGMNPQIERMKRGVDILVATPGRLLDLQQQGFMDLSTVEILVLDEA
                     DRMLDMGFIHDVKKVLALLPASKQSLLFSATFSDEIRDLAGNLLKNPQSIQVTPRNTT
                     VQRISQIIHPVGRAKKKQVLLHIIQQHDWSQVLVFTRTKFGANNVADYLTKNGVTAMA
                     LHGNKSQSARTQALEGFKTGQIRALVATDIAARGIDIDDLPHVVNYEIPNVSEDYVHR
                     IGRTGRAGREGQAVSLVCMDEEGFMMEIERFTKQEIPVQVIDGFGPDEDEKAEPIAMG
                     RQTIWGGAGKPPSREVMQAAAKAARSEMMERIRTNKAGQGQGQPRGGRQGGAGKGAQG
                     EGARAANGQGGQGRGGNAGRPAGPARGQQQQPRGADGGRRSGNNPGNRRHDDSQPRSE
                     NAHLGTQLRHVSLDPRHGGSGRTSQPDPMRTSVDSMADRGRRGGYRGGGGGGGRGPRG
                     GGGFGR"
     gene            12234..14096
                     /locus_tag="PK518_08680"
     CDS             12234..14096
                     /locus_tag="PK518_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRM49031.1"
                     /translation="MMTHKFRTHSTTWPSAATALRISAPACRSGAAALLALVLAACSS
                     TPLPPWPAQETSQSAAAPQAQRQVPPPLGTGASTAPARTEAIASPVTVTPLPGTPPAA
                     EIPMPQQPYGAAVAARFPDPSVRYETPGLADGRRAFTTNAELTQWLRQLATAQRGATR
                     TQLLDLGLSQRGTPIHALVITRTAATDAAALAASRRPTVLLIGQQHGDEPAGSEALLV
                     VARELAQGLLEPMLDRINVIVVPRANPDGADAGKRVTANGIDMNRDHLLLETPEAQAL
                     ARLVRDYRPIAVIDAHEYTVAGRFLEKFHALQRYDMLLQHATTANLPEFMSKAALEWY
                     RQPMLKALDTQGLTQEWYYTTSTQPEDLRLSMGGTQPDTGRNVNGLKNTVSMLLETRG
                     IGIGRSHIQRRVHSHVTAVTSALRSTVDRAGNLEQVRSYETRDIAAQACRGEIVVEAG
                     PTPTQRELLMLDPETGADRPLRVDWNSSLQLQTLKKRPRPCGYWLAGHNDKAVERLKL
                     LGIQVMRVAEAGSTLADTYHETRRESADRQDVRGTVAGAAPIIRVQVTPTRSAIDVPA
                     GSYYVPLNQPLANLAVAALEPDTQNSYFANHLITNLGDLARVMTPPALVFEETE"
     gene            14417..14493
                     /locus_tag="PK518_08685"
     tRNA            14417..14493
                     /locus_tag="PK518_08685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14451..14453,aa:Arg,seq:tct)
BASE COUNT         2383 a         5185 c         4789 g         2130 t
ORIGIN      
        1 caggctggtc gcctgggcgg cgcagcgcag cagctccagc tgggccagcg catcggggcg
       61 gccggtgcgc gccagctcgg cgcgggcgcg ctgccactct tgagcagcca cgcggtcatt
      121 gcccgccagg taggcggcgc tggcgcgctc ggccgcgcca tgggcgttct gctgccagtc
      181 gggcacgggc ggctggttgc tgcaggcggc caataacgcc gccagcagga cggccagaaa
      241 aaccctgcat gagactggcg cgctccatgc gcgggcagcc gcgcaagggc cgccccgccg
      301 cgctggctgc gtcctcctgc ccggcgaagc cgagagcggg ggctgagggc cgcggctcca
      361 agcctgcctg cgcaggcttg gacggccctg aagagctgcc gcctctggcg gctgcacgga
      421 ggcgcggagc gactcagggg ggtgttgttt catggcagcc tcagctcctg gtcgcgcgca
      481 aaaggccatt tgcggttgat gtcattgatc aggccctcca gcttgcgcag gttggcctcg
      541 acctcggcgc gcagcgcgcc caggtcggcg ctggcctcgc gcgcgttggc gcccacggcc
      601 tgggcctcgg ccagcaccgc gtccacgcgc gtcaggctct ggcgcgtgtc ctgcagcagg
      661 ccgcttgcct gcgccacagt ggcgcgcagc tcggccacca ggccgggctg gccggcggcg
      721 ccaaacacct ggcggtcggc ccgggccacc acggcgtcca tgcgcgccag cagctgctgc
      781 gtgcggtcca gcgtctgtag caccttgcgc gcatcctgct cgttgcccat gagcacgccc
      841 agcgcgccgc ccggcgcgtt cagccgctgc gtgagctggc gcacctgggc cagggtgtcg
      901 ttcagcgccg aatccggggc gctgagctgc tgcaggttgt cgagcagctg gcgcgccgtg
      961 gccatgagct gcgggatctc ggccgtggcg tcgccgcgca gcaccgggcg ctcggcgcca
     1021 gcggccagcg gcgggtcggt cagcacgccg ctgtaggcct tgatggtcgt gccgcccacc
     1081 aggccgcgca ccagggtgaa cacgctggat tcgcgcagcc agtgggcgtc gcgactggcc
     1141 acgtccacgg cgatgcgcac attgccgtcg cccgccagct cgatgcggcg cacgcggcct
     1201 atgggaaagc ccgagaacgt catgtccatg cccacggaca cgccctcgga gtcgtcggca
     1261 atcagcacca ggcgctgggt gggctcgaag gcgccgcgcg catacagcag gtacagcacc
     1321 gagccggcga tcagcgccag cgtgagcagc agcagggccg cggccttcag gcgcagatgc
     1381 gccacggggc gcagcagctc ctcggggggt ggaggtggca gggtttcagg catggctcat
     1441 tcgtgattca gtagtaatta cccatcagcg acagcgcctc aatgagcagc agcacggcaa
     1501 acaggcgcgc caggctggcc ggtgcggcgc cgtcctggcc cagcagggcg ccgggcggtg
     1561 gtgggtgcag gccgccggcc atggggatca gcgccacggc caggctgaac agcagggtct
     1621 tgagcgtgaa caccagcgtg atcgagggcg tgaagacctg gccgaacatg cgcgtgtacg
     1681 cgctcaatcc tgccgtggtc aggccgtaca cgcccagata agccatgacc agcgccacca
     1741 cgcaggacag cgccgccagc gtcacgctgg cataggcgcc ggcgagcacg cgcggcagca
     1801 actcgacacg cagcgggtcg gcgccgcgtg cgcgccaggc ctgcagcacc cccgcctggc
     1861 gcagccgcgc cagctgcgcg ccgctgggaa tggtggtgcg catggcgacg aacagcgccg
     1921 cggtgagcgg tatcagctcc agcaccagca cgcgtatcac catctccagc gcatagcgcg
     1981 tcaggccgta gctgagcgcg gtgacgacga cgatatgggt gatgaccagg ctgagtagcg
     2041 ccgccagggc ggtgaagccc ggcagtatgg gcagcgtgtc ggtgtagatc tgccgcgcca
     2101 ggcgatcgcg cgcgccgcgg ccatagctgg acggcgacag cagcagcacc agcaccacgg
     2161 cgcccaggtc gatgatgcgc caccagccca gcgcccagcg ctgcgcgccg cgccacaggc
     2221 ggtcgaggct ggcgtcaaac atggcgcagc gccgaagcgc tgggttgcgg cggcaggtcc
     2281 tgcgcctcgg cacgctcgtg cagggcttct atcacctggc aatggtcggc ctggccatcg
     2341 cagcggccgc gcaggccgcg cagctccttt tccagcacgc gcagctcggc caggcgctgg
     2401 cgcacatgag ccaggtgctc atccagcgtg gcgcaggcct ggtggtcggc ctggtcgctg
     2461 gcggcgtcta tggccagcag gcggcgcacc tcatcgagcg acatgtccat ggcccggcac
     2521 aggcgtatga agcgcagccg gtgcaggtct gccgaactat aaaaacgata gctgttgtcg
     2581 ccccgcacac ctgggcgcag caggccttcc ttctcgtaat agcggatatt cgcggcggac
     2641 acgcccgaca gccgggcggc gtcgcctatg cggtgctgcg ggctttgctg cggcatgctt
     2701 gaccttgaag tggcttgaag gtttccaatc atcgcatgaa ccatagcacc agccctcttg
     2761 gccatgacca tggccatgcg catgacaggc cgcacacgca tgaacacggc gccgccaaca
     2821 ccgcctgcag cgcctgcagc agctgcgcca ccgacgacga ccatgaccac ggcacgctgc
     2881 cgccctggcc gcgcattgcc gccgcgctgg tgttggccct gggcgccgaa gccgcccact
     2941 ggctgcagtg gacatggccc ggcatggccc tggccgtagc ggccatcgcc ctgtccggcc
     3001 tgggcgtgta ccgcgccggg ctcaaggacc tggcgcggct gcgcctgggc atcaacgcgc
     3061 tgatgaccgt ggccgtgacc ggcgccgtgc tgatcggcca atggcccgag gcggccatgg
     3121 tcatggcgct gtacgtggcc gccgagcgtc tggagcatgg cgctatggac agggcgcgca
     3181 acgccatccg cgacctgctc aagctcgccc cggccacggc cgatgtgctg caggccgacg
     3241 gcagcgtgca gcgcctgccc gtggcccagg tgccgctggg cgccacgctg cgcatcgccc
     3301 ccggcgccca tgtgccgctg gacggccagg tcacgcaggg cagcagcgcc gtgaaccagg
     3361 cccccatcac cggcgagagc cagctggccg acaaggcgcc gggcgacgag ctgtacgccg
     3421 gcagcgtgaa ccagcatggc gagctgctga tgcgcgtcag cgccgcgccg gccgacacgc
     3481 tgctggcgcg catcgtgcgt gccgtcgagc aggcccaggc cagcaaggcg ccggcgcagc
     3541 gcttcgtgga tcgctttgcc gccgtctaca cgcccatcgt cttcgtgctg gcggtgctgc
     3601 tggcgctggc ggcgccgcag ttcatgggct ggagctggca gcaggccgcc taccaggcgc
     3661 tggcgctgct ggtgatcgcc tgcccctgcg cgctggtcat ctccacgccg gtgacggtgg
     3721 tcagcgccct caccgcggcg gcgcgccgcg gcattctgat caagggcggc aacgcgctgg
     3781 aggcggcgcg cggcctgcgt gcggtggcgc tggacaagac cggcacgctg accaccggca
     3841 acccggcgct ggtgcagtgg caggcgctgg cgggttttga cggcgcccag gccgcggcca
     3901 tcgcctggca gctggccagc cgctctgagc acccggtctc gcgcgccatc gccacgggcc
     3961 tgccggccgt ggcgcacaac ggcgtgaccc agctacaggc cctgcccggc cgcggcgtgc
     4021 aggccgacat cgacggccag cgctggcagc tcggcaacct gcgcctgatg cgcgagcagg
     4081 gcctggccga cgcggccctg ctggccacgc tggaggagca cgaacgccag ggtcgcagca
     4141 tcaccctgct ggcaggcggc ggcgccgtgc atgcctggtt tgccgtggcc gacccgctgc
     4201 gcgcccatgt gccccaggcc atcgcccagc tgcgcgccct gggcgtgcag cccatggtgc
     4261 tgagcggcga caacccggcc accgtgcagg cgattgcgct ggaggccggc atcagcgccg
     4321 aggacgcgcg cggcggcctg ctgcccgagg acaagctggc cgccctggcc gagctgcagc
     4381 accgccacgg ccccacggcc atgacgggcg acggcatcaa cgacgcgccg gccctggcgc
     4441 gcgccgacct gggctttgcc atgggcggcg cgcacagcac ggccatggcc atggagacgg
     4501 cgggcgtggt gctgatgcac gacgacctgc gccgcatacc cgacaccctg cgcctagcgc
     4561 agaaaacgca ccgcgtgctg tggcagaaca tatccctggc gctgggcgtg aaggcggttt
     4621 tctttatgct cgcgctgctg ggcctggcca ccatgtggct ggccgtgctg gccgacatgg
     4681 gcgtaagcct gctggtggtg gccaacggcc tgcgcctgcg ccacgccctg cccgccactt
     4741 ccgaccagcc acgaccatga gccagacccg ccaacgcgcc gccttcgact atctgctgca
     4801 gcaatttgcc gccagcgccg gccagagcgc cgcccagcgc tggcgctacc tggaggccgc
     4861 ccatgtgctg ggccagaacc gcctggccct gcacctgcgc gcgcactggc agatgctgcg
     4921 ctttgcgcag gcgcttggcg accagcatga agcccggggg cagctcagcc atctgtgcat
     4981 ggccccggtg ggccatatcg tgcggcgcat gccctacggc aacaacggcc gcgccatcgt
     5041 gcccgcgctg caccccatgg tgcccagcgc cgcgctgcgc agctgcatca gtgaagccat
     5101 gcaggccgtc ggaggtgatg taccgcaaga cccggcgtaa aaaccacccc gcccggcggc
     5161 ccatagccgt cgaaagggca tgccgagcaa ggaaaatggc gccccatgtc cgccaccatc
     5221 accaccccgg cccccgctcc ccacacccgc gttgaagacc tggtggccat cgtcaccggc
     5281 gtggccatgg tctcggtggg cgtggcgctg gtgggcagcg ccggcttgct caccggcggc
     5341 atcaccggcc tggcctttgt gctgcactac gccaccggca taggctttgg caagctgttc
     5401 tttgtactca acctgccctt ttactggctg gcgctgcgca agctggggct gggcttcacg
     5461 gtcaagacct ttgccggcgt ggccctgttg gccgtgctga ccgaggtgca gccgctgctg
     5521 atgcaggtcg gcagcatcca gccgctgtat gccgcgctgg tgggcgggct gtgcatgggc
     5581 atgggctttc tggtgctgtt tcgccaccac tgcagcctgg gcggcatagg cattctggcg
     5641 ctgtatctgc agggccgctt tggctggcgc gcaggcgcag tgcagatggc ggccgacttc
     5701 tgcatcgtcg ccgccgccct ggtcaccgtg gagcccatgc gcgtgctgtg gtccgtcatc
     5761 ggcgccgtgg cgctgaacca ggtgctggcc atcaaccacc ggcccgggcg ctacatggcg
     5821 gcctgaggaa cttgtcaagc accccggtat cattcagccc atgcgccgcg ttctctcctt
     5881 tgccctggtc atgctgctgc tgatgcgcgg cctgccgggc gacgccatgg cgctgggcca
     5941 ggcaccggtg cagcccacgc cgcagatggc gcagatggcg cacgccatgg acgccgacgc
     6001 gcaccatgcc gccgccatgg cgcaggactg ctgcgaccac gccagcgaca gccatatggc
     6061 gcaccagggc ggctgcagcg cctgcggcgt ctgccattca acgctggctg tttccccctg
     6121 gatggcagtg cccggcacgg gcgcgcagca cagcccgcaa gcacctcccg gcacgcggtt
     6181 taccagtgcc ctggctgctc aggccatcaa gccacccatt ttctgagacc cgacgcccga
     6241 agtgcgtccg cagacatccg tatccgccct ccgtggcggg tgcgcggcat ggcgcatgcg
     6301 cccatgcatc cctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgc
     6421 gccgcagcag caggcctggg gcattgccgg ccaggccggc aaggccacgc gcaccatcac
     6481 cctgcgcatg acggacgaca tgcgcttcac gccgtcgcac ttcagcgtca agcaaggcga
     6541 gaccgtgcgc ctgcgcgtcg agaacaaggg ccaggtgctg catgaggtgg tgctgggcac
     6601 gcaggccagc ctggacgagc atgcggccat gatgcgccag cacccggaca tggagcatgg
     6661 cgaggcccac atggcccatg tcaagcccgg ccgcaaggac gatctggtgt ggaacttcaa
     6721 ccgcaccggc agcttcgact ttgcctgcct ggtcgccggc catttcgagg ccggcatgcg
     6781 cggcagcttc accgtcacgc cctaatcgca acctgcccgg agaacacagc atgcacaaca
     6841 ccacccgccg ccaatggctc acggccatcg ccgccgccag cgcctgcgcc gccctgcccg
     6901 cctgggccac cagcaacgcc aacgccgtcg aggtctggaa agaccccagc tgcggctgct
     6961 gccaggactg gatcgatcac atgcaggcca acggcttcaa ggtcaccgtg cacgaaaccg
     7021 gcaacgccgc cgtgcgcgcc aagctgggcc tgccccagcg cctgggctca tgccacacgg
     7081 ccctggtcgg cggctacctg ctcgaaggcc atgtgcccgc cgccgacgtg cacaagctgc
     7141 tcaaggaaaa gcccaaggcc ctgggcctga cggtgcccgg catgcccgtg ggcagccccg
     7201 gcatggacgg ccccgagtac aagggccgca aggacgccta cgacgtgctg ctggtgacca
     7261 agaacctgat gaacaaggat gtctccaccc gcgtgttcac cagctaccgg taacgggtaa
     7321 cgggtagcgg cccgccggat gggctatcgt ggtgttttct ctgcccgatg cccagccatg
     7381 catgatccat ccgaccccgg cgccatcacc catgttgccg gcatagaggt aggccaccac
     7441 agcgacagtc gccgccccac gggctgcacc gtggtcatgg cgcgcgcagg cgccgtggcc
     7501 ggcgtggatg tgcgcggcgc cgcacctggc acgcgcgaga ccgatctgct ggcccccggc
     7561 aatctggtgg acaaggtgca cgccgtgctg ctggccggcg gcagcgcctg ggggctggac
     7621 gccgccacgg gcgccgtgcg ctggctggag gagcaaggcg tgggcctgga cgtgggcgtg
     7681 ggccgcctgc ccctggtgcc cgccgccgtg ctgttcgatc tgcatgtggg cgacatgaag
     7741 atccgccccg acgcggcggc cggctacgcc gcctgcgccg ccgcctcacg ccaggcgccc
     7801 gaagaaggca acgtgggcgc gggcagcggc gccgtggtcg gcaagatgtt cggcatgcag
     7861 cacgccatga agggcgggct gggcacggcg tcggtcaccg tggccggcgt gacggtgggc
     7921 gcgctgatcg cctgcaacgc cgtgggcgac gtggtcgatc ccgccaccgg cctgcccctg
     7981 gcaggcgcac gcacggccga cggacaagcg ctgcgcgaca cccgccgcgc cctgctcgcc
     8041 ggcgcaccac cccacccgct gctggccggc agcaacacca ccattggcgt catcgccacc
     8101 gatgcgcccc tctccaaggt acaggcccag cgcctggccg tcgccgggca tgacgggctg
     8161 gcgcgcgcca tcaaccccgt gcacaccatg agcgacggcg acacactgtt caccctggcc
     8221 accggcgtgc gggagcagca tcccggcatg ctggtgctgg ccaccatggc cgccgaggcc
     8281 gtggcccgcg ccaccgtgcg cgccgtgctg accgcgcgcg cgctgcgtct gaacgatggc
     8341 ctgcggctgc cctgccacgc ggacatggtc agcaccgggg gctgatagtt cctgccgcca
     8401 gcggcaggcg caaaaaacaa cgggcctttc ggcccgttgg tcgtcacggc atttcgcgcc
     8461 ttatttgagc gccttgtagc gcatgcgctt cggggccgcg ccctcttcgc ccaggcggcg
     8521 cttcttgtcg gcctcgtact cctggtagtt gccgtcaaag aacacccact ggctgtcccc
     8581 ttcggctgcc aggatatggg tggcgatacg atccaggaac cagcggtcgt ggctgatcac
     8641 cagcaccgtg ccggcgtatt cgagcagcgc gtcttcgagc gcgcgcaggg tttccacgtc
     8701 caggtcgttg gagggctcgt ccagcagcag cacgttgccg ccctggatca gcgtcttggc
     8761 caggtgcagg cggccgcgct cgccacccga gaggttgccg accttcttct gctgatcctg
     8821 cccgttgaag ttgaagcgcc cgcagtaggc gcggctggcc atctggaatt tgcccacgtt
     8881 gatgatgtcc aggccaccgg agacgtcttc ccacaccgtc ttttcattcg ccagcgcatc
     8941 acggtgctga tccacgaagg ccatcttcac ggtctggccg atctccaccc cacccgaatc
     9001 aggttgctcc ttgccggcga tgagcttgaa cagcgtcgat ttaccagcgc cgttcgggcc
     9061 gatgatgccg acgatggcgc cagccggaat gttcatcgac aggttgtcga tcagcaggcg
     9121 gtcgccaaag gccttggaga cgttgttgaa ctcgatgacc ttgctgccca ggcgctccgc
     9181 cacggggatg aagatctcct gcgtctcgtt gcggcgctgg tattcgtagt cggacagctc
     9241 ctcgaaacgt gcgatacgcg ccttggactt ggcctggcgc cccttggcgt tctgacgcac
     9301 ccactccagc tccttcttca gggccttggc gcgcgcttcc tcgccctttt gttcggcctc
     9361 caggcggttt tgcttctgca gcagccagtc ggaatagttg cccttgtacg gaatgccgtg
     9421 gccacggtcg agttccagaa tccactcggc ggcgttatcc aggaaataac ggtcgtgggt
     9481 aatggccacc acggtgccgg tgaagcggtg cagaaactgc tccagccact cgacggactc
     9541 tgcgtccagg tggttggtgg gctcgtcgag cagcagcatg tcgggcttgg acagcagcag
     9601 ctggcacagc gccacgcggc gcttctcgcc acccgagagc tggccgatca ctgcctccca
     9661 gggcggcagg cgcagcgcgt cagcggcgat ctccagctgg tgctcgctgt cggtgccggc
     9721 tgcggcgatg atggcctcca gcttgccctg ctcggccgcc agggcgtcaa agtcggcgtc
     9781 ttcttcagcg taggcggcgt agacctcctc caggcgcgcc ttggcgttgt tcacctcggc
     9841 catggcctct tccacggcct cacgcaccgt gtgctcgggg ttgagctgcg gctcctgcgg
     9901 caggtagcca atcgacaggc cctgcatggg gatggcctcg ccctcgaact ccttgtcgat
     9961 gccggccatg atcttgagca gcgaagactt gcccgagccg ttcaggccca gcacgccgat
    10021 cttggcaccg gggaaaaagg acagggaaat gtctttcaag atctgccgct tcgggggcac
    10081 ggtcttggtg acacggttca tcgagaaaac gtactgggcc atggattctt taaaaaaatg
    10141 agcaaagaaa ggcgccgaaa atgcggcgcg caaccgtgga ttatcggcgc atgcgacaat
    10201 agcgcccgct cggggttcca gttgccccga catcagccta gacaagctgg ggacgacgaa
    10261 agtatttttc tggctcccac ccttgaaatc atcggcctac agaactggtc ggcgacccac
    10321 ccggtcgccc ggcaacaggt cgatacccag cgcccaggat gggcgcatcc aacctgaata
    10381 atgacattcg aagaactgaa gctggcgccg gccattttgc aggccgtgca cgaacagggt
    10441 tacaacaacc ccacccccat ccaggcccag gccatccccg ccgtccttgc cggccatgat
    10501 ttgctggccg gcgcacaaac cggcaccggc aagacggcag ccttcacgct gcccatgctg
    10561 catcgcctgg ccggcagcgc ggccgccacg agtaagtttg gcaaccgtgg cgtgcgcgcc
    10621 ctggtgctca cccccacgcg cgagttggcc gcacaggtcg aggaatcggt gcgcacctac
    10681 ggcaagcacc tggcactcaa gtccaccgtg atcttcggcg gcgtgggcat gaacccacaa
    10741 atcgaacgca tgaagcgcgg cgtggacatc ctggtcgcca cgcccgggcg cctgctggac
    10801 ctgcagcaac agggtttcat ggacctgtcc acggtcgaaa tcctggtgct ggacgaggct
    10861 gaccgcatgc tggacatggg cttcatccac gacgtgaaaa aggtgctggc gctgctgccc
    10921 gcatccaagc aaagcctgct gttctcggcc accttcagcg acgagatccg cgatctggcc
    10981 ggcaacctgc tgaagaaccc gcaaagcatc caggtcacgc cacgcaacac cacggtgcaa
    11041 cgcatcagcc agatcatcca cccggtcggc cgcgccaaga agaagcaggt gctgctgcac
    11101 atcatccagc agcatgactg gagccaggtg ctggtgttca cgcgcaccaa gttcggcgcc
    11161 aacaacgtgg ccgactacct gacgaagaac ggcgtcacgg ccatggcgct gcacggcaac
    11221 aagagccaga gcgcccgcac ccaggcattg gaaggcttta aaaccggcca gattcgggcg
    11281 cttgtagcta cagatattgc agcacgcggg atcgacatcg acgacctgcc gcatgtcgtc
    11341 aactacgaga tccccaacgt ctccgaggac tacgtgcacc gcatcggccg caccggccgc
    11401 gccggccgcg agggccaggc cgtgagcctg gtgtgcatgg acgaagaagg cttcatgatg
    11461 gagatcgagc gcttcaccaa gcaggagatc ccggtgcagg tcatcgacgg ctttggcccc
    11521 gacgaggacg agaaggccga acccatcgcc atgggccgcc agaccatctg gggcggcgcc
    11581 ggcaagccgc cgagccgcga agtcatgcag gcggcagcca aggccgcacg ctccgagatg
    11641 atggagcgca tccgcaccaa caaggcgggc cagggccagg gtcagccgcg cggcggccgc
    11701 cagggcggtg ctggcaaagg tgctcagggc gaaggcgcgc gcgcggccaa tggccagggc
    11761 ggccagggcc gtggcggcaa tgccggccgg cccgcaggcc cggctcgcgg ccagcagcaa
    11821 cagccccgcg gcgctgacgg cgggcgtcgc agcggcaaca acccgggcaa ccgccgccat
    11881 gacgacagcc agccgcgcag cgagaacgcc catctgggca cccagctcag gcatgtgagc
    11941 ctcgaccccc gccacggcgg cagcggtcgc accagccagc ccgacccgat gcgcaccagc
    12001 gtggacagca tggccgaccg tggtcgccgt ggcggctatc gcggcggcgg tggcggtggc
    12061 ggacgtggtc cgcgcggtgg cggcggtttt ggccgctgac acagagctgt caaactgcgc
    12121 tgacaagctc ggcccacgcc gggcttgttt gcatctgggg ccgcgcgcgc tgcctgccct
    12181 ggccggcatc agatgagtgc cggctaacat cggcacattt cactctgcca accatgatga
    12241 cgcacaagtt ccgtacccac agcaccacat ggccaagcgc cgccacggcc ctgcgcatca
    12301 gtgcgcccgc ctgtcgcagc ggcgcggcag cactcctcgc cctggtgctg gccgcatgct
    12361 ccagcacacc actgcccccc tggccggcgc aggagaccag ccaatccgcg gccgctccgc
    12421 aggcccagcg gcaagtcccg ccgccgcttg gcacgggcgc cagcacggcg cctgcgcgca
    12481 ccgaggccat tgcctcgccg gtgaccgtta cgcccttgcc tggcacgccc cctgcggcag
    12541 aaatccccat gccgcaacag ccctacggcg cggccgtggc ggcacgcttt cccgatccgt
    12601 ccgtgcgtta cgaaacgccc ggcctggccg atgggcgccg ggccttcacc accaatgccg
    12661 agctcacgca atggctgcgc cagctggcga cagcacagcg cggcgccacg cgcacgcagc
    12721 tgctcgatct gggtctttcg cagcgcggca cgcccatcca tgcactggtg atcacacgca
    12781 ccgcagccac cgatgccgcc gcactggctg ccagccgccg ccccacggtg ctactcatcg
    12841 gtcagcagca cggcgacgag cccgcgggca gcgaggccct gctggtcgtg gcgcgcgagc
    12901 tggcccaggg gctgctggag cccatgctcg accgcatcaa cgtcatcgtc gtaccgcgcg
    12961 ccaatccgga cggcgccgac gccggcaagc gcgtcaccgc caacggcatc gacatgaacc
    13021 gcgaccacct gctgctggaa acgcccgagg cccaggccct ggccaggctg gtgcgcgact
    13081 atcgccccat cgccgtcatc gacgcacacg aatacacggt ggccgggcgc ttcctggaga
    13141 agttccacgc cctgcagcgc tatgacatgc tgctgcaaca tgccaccacc gccaacctgc
    13201 cggagttcat gagcaaggcc gcgctggagt ggtaccgcca gcccatgctc aaggcactgg
    13261 acacccaggg gctgacacag gagtggtact acaccacctc cacccagccg gaggatctgc
    13321 gcctgtccat gggcggcacg caacccgaca ccgggcgcaa cgtgaacggc ctgaagaaca
    13381 ccgtcagcat gttgctggaa acgcgcggca taggcatagg ccgcagccat atccagcgcc
    13441 gcgtgcactc gcatgtcaca gccgttacca gcgcgctgcg cagcaccgtc gatcgcgcgg
    13501 gcaacctgga gcaggtgcgc tcctacgaaa cgcgcgacat cgccgcccag gcctgccgtg
    13561 gcgagatcgt ggtcgaggcc ggccccacgc cgacccagcg cgaactgctc atgctggacc
    13621 cggaaaccgg cgccgatcgt ccgctgcgcg tggactggaa ttcgtcactg cagttgcaga
    13681 cgctgaagaa acgcccacgc ccctgcggct actggcttgc gggccacaac gacaaggccg
    13741 tcgagcgcct gaagctgctg ggcatacagg tcatgcgcgt ggccgaagcc ggctccaccc
    13801 tggccgacac ctaccatgaa acccgccgcg aaagcgccga ccgccaggac gtgcgcggca
    13861 ccgtggccgg cgccgcgccc atcatccgcg tacaggtcac ccccacccgc agcgccatcg
    13921 acgtacccgc gggcagctac tacgtgccgc tgaaccaacc cctggccaac ctggccgtgg
    13981 ccgcactgga gcccgatacg caaaacagct acttcgccaa tcacctgatc accaacctgg
    14041 gcgacctggc acgcgtgatg acaccacccg ccctggtatt tgaggaaacc gagtgaaagt
    14101 caccgcacac caggttaatt tactatgata aatatagctg cttgcgcttt actgcattgc
    14161 ctttgagcag gttttctctg atatttgatt gccacaggat catggcctcg cgatacccat
    14221 caaagcgcac gccaatccgc ctgagcgggc agtacaaccc gctatgattc agccccttgg
    14281 cgacgctaac cgccggtggc tcaaccggcc acggtgcgca tgcctgcaaa gcctttggtc
    14341 ggcaagctgg aatcggttgc acgacaaggc gttttgaacc acacgcccac cccggcggaa
    14401 cctcaggggc acctaagcga cgttagctca gttggataga gcagcggtct tctaaaccgc
    14461 aggtcggggg ttcgatcccc tcacgtcgcg ccaaacaccc cgccatgcgg ctggaaaaca
    14521 tcaccgtggc gcccacagcc gtagagacaa ttcggccctg gacgagcagt cccgccctca
    14581 catgtag
//
LOCUS       DAOMTQ010000073        14521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_678_length_14521_cov_26.329877, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000073 DAOMTQ010000000
VERSION     DAOMTQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_678_length_14521_cov_26.329877"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PK518_08690"
     CDS             complement(<1..880)
                     /locus_tag="PK518_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRM49032.1"
                     /translation="MNNWQQRIAALPAERLAGMRRGIEKEGLRVLPEGGLALTPHPQA
                     LGSALTHASITTDYSESQIELITGAHQSVSGCLDELIHIHQYVLRTLAGAGGEMLWVS
                     SMPCGLPTDETIPIGRYGNSHIGRAKSIYRMGLAHRYGRRMQTISGIHYNWSLPGVTS
                     EEYFALIRNFRRHAFVLLYLFGASPALCPCFVEGRAHSLQPMGDGSQALHLPHATSLR
                     MGRLGYQSDAQASLAVSYNGLSGYADSLYEALTRPYPAYEAIGIRNIGGDYNQLGTSL
                     LQIENEFYGTIRPKRTV"
     gene            complement(1148..2071)
                     /locus_tag="PK518_08695"
     CDS             complement(1148..2071)
                     /locus_tag="PK518_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM49033.1"
                     /translation="MTLDFQGRVAIVTGAGGGLGRQHALALAARGARVLVNDLGGAVD
                     GSGGSVGAAQAVADEIRAAGGQALANGASVTDFAAVQAMVQQAQDAWGRVDILVNNAG
                     ILRDKSFAKMDMADFALVVQVHLMGAAHCCKAVWPLMQAQNYGRIVMTTSSTGLYGNF
                     GQANYGAAKLAQAGLMQTLAIEGAKYGIHVNALAPTAATRMTAGLLPDEVMAAIRPEA
                     VVPAMLVLAHESAPTRTILCAGAGTFEAAHITLTQGLYLGVGDDVPEQLAARLAEVCE
                     RHGEQVPASGAAQGQNEIRLAMAAAEAGVSA"
     gene            2264..4045
                     /locus_tag="PK518_08700"
     CDS             2264..4045
                     /locus_tag="PK518_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HRM49034.1"
                     /translation="MTDTDTGTTLLDLNLDRTLDLWLRRFSQPQWRGRHVEGWLFEGQ
                     AERRAAEQRLAAVGVQARFHSAYKPLVQHFIDNVGRAQLVAAHVRYPVPQIGPAQRFR
                     LEAYPLAALLPEGGLTLEPRADALLHYDVELELQGGKRLTQRVHAPNRRHADASGAPA
                     LSPTGWLRVGTMAGAANLLDCAVSTDYEQAFAQALHAVRAHPWPDAEPYFERLQLRID
                     LPGYEQPIAWAGESISTAEALHEDLYFSLLEFFQQHSGRPLGDRRLQPGQIVPDIRLG
                     ATTPRVRVSLQPFDAQAQDALAAAQLPQAAAAALESLGSAPAPAYIAQILQGWPGQRF
                     EAPTRQGRSVWGYYQAGTQRPVFISGGQHANETSGVVGALRAADTLRHDGRAHFALIA
                     LENPDGYALHRELCCQHSGHMHHAARYTALGDDVEYREGAPLFERAARQQALALSGAQ
                     LHVNLHGYPAHEWTRPLSGYVPRGFEQWMCPKGFFLILRYHAGWQQPARTLLEAVCAR
                     LKAVPGLAEFNARQRALYEQHAGPMPFELIDGIACTQAQTTHDAPVTLVTEFPDETVH
                     GDAFRFAHLVQMHTVLAAVQAWQQIEL"
     gene            4074..4463
                     /gene="crcB"
                     /locus_tag="PK518_08705"
     CDS             4074..4463
                     /gene="crcB"
                     /locus_tag="PK518_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRM49035.1"
                     /translation="MLHALAICLGACAGALARWRLSLWLNTQGALLPWGTLAANLIGG
                     YLIGLCLALFQALPQLDPAWRLLLVTGFLGALTTFSTFSAEVLGLLQQQRLALALGLA
                     TLHVAGSLCMTWAGLQSAALLRLESRF"
     gene            4592..5455
                     /locus_tag="PK518_08710"
     CDS             4592..5455
                     /locus_tag="PK518_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRM49036.1"
                     /translation="MQANTDLQDSRLANAWAELHNYAVAGNPLHADSYRLAFADPEFM
                     LRRETRGIRFQLELLKPELEQADQGIEHTVVVYGSARFVAPEDAAEQLAEAQASGDAE
                     RIARAERAVKNAYYYDQARRFARHVAEYSQTRPPAERLYICTGGGPGIMEAANRGAHE
                     AGAPNIGLNITLPHEQSGNKYITPSLSFKFHYFALRKMHFMMRAKALVLFPGGFGTLD
                     ELFEVLTLVQTGKAKAAPIVLFGSEFWKRLINFDALVEQGTISQGDLKLFHYCDDPAE
                     AWAIVRAFYEL"
     gene            complement(5488..7029)
                     /gene="rmuC"
                     /locus_tag="PK518_08715"
     CDS             complement(5488..7029)
                     /gene="rmuC"
                     /locus_tag="PK518_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRM49037.1"
                     /translation="MTLEMMIALALLVLVVLLLCALLLRRPRVALPPEWPQRLQALES
                     AVQATQLAVAKNDGALDAMGQQLRGFTHSTQVLLDERLQLAAQESRTSRAELQAAFGA
                     LQQRLEQQLVQGRSDEAQARHEQLQALAAFRAELTQTAQALKGDVQAQLEAHTQQLRH
                     QFAGLQEAVGQQLTGLAASSQQGAEALRAALNERLAAIQQDNAAKLEEMRRTVDEKLH
                     ATLEQRLGDSFKLVSERLELVHKGLGEMQTLAAGVGDLKRVMTNVKTRGTWGELQLGA
                     IIDNVLTPGQYEKNVKTQPGSDELVEFAIRLPGRSDDQPVWLPIDSKYPVEHYQRLVD
                     AQDQADKGAIAAAGNAFEASIKLEAKKIFSKYVAPPYTTDFAVLYLPTEGLFAEVMRR
                     PGLVEAVQNDCRVMITGPANLAAMLSSLQMGFKTLAIEKRSSEVWGVLGQVKSEFAKF
                     GVIVDQTRKSIDAAARKFEQVDVRTRAIQRRLRDVQELPAPGQQALDDAGASAEDLLI
                     EDEGD"
     gene            complement(7095..8072)
                     /locus_tag="PK518_08720"
     CDS             complement(7095..8072)
                     /locus_tag="PK518_08720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRM49038.1"
                     /translation="MSKPRILVTRHIFPEITDYLAQHFELESNPDDVLWSQAELAQRL
                     ADKDGVFITGSHRIDAALLAACPRLKIAANMAVGYNNFDVQAMTAAGVQGTNTPDVLT
                     ETTADFGFALLMATARRMTESEHYLRAGLWNSWRYDMFAGAEVHGSTLGILGMGRIGQ
                     GIARRGAHGFGMQVIYHNRSRLAPELEAECKARYVSKDELLAQADHLVLVLPYSAESH
                     HAIGAAELARMKPTATLVNIARGGIVDDAALAQALKEGRIAAAGLDVFEGEPAVHPGL
                     LERSNVVLTPHIASATVPTRLAMARLAADNLVAFFDGRGALTPVNTPNQ"
     gene            complement(8093..9508)
                     /locus_tag="PK518_08725"
     CDS             complement(8093..9508)
                     /locus_tag="PK518_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRM49039.1"
                     /translation="MKLFRTLTLTALSLAPALAAAADIDGGQLSLLWGVPFAGILLSI
                     ALLPMVAPLFWHHHFGKLVVGWSLAFLLPFAVVFGPQAAGAQFVHALAAEYLPFVILL
                     SALFTVAGGIYIRGNLRGSPVLNTGILLVGAVLASFMGTTGASMLLIRPLIRANDNRR
                     HVAHVVVFFIFVVSNAGGSLTPLGDPPLFLGFLKGVDFFWTLRHIFPETLFLIAVLLV
                     LFFAIDSWYFRQDGELRRTDPTPDSGGAALGFDGKINFVLLLGVVALVLMSGMWKSSV
                     QWTIAGTPVGLPGLLRDLGLIAITLVSLVLTPRQVHSDNQFGWGPMQEVAKLFAGIFL
                     TIIPVIAMLKAGVNGPFGAIVSAVTRADGQPDPAMYFWATGLLSSFLDNAPTYLVFFN
                     TAGGDPATLMTTYATTLAAISAGSVFMGANTYIGNAPNLMVKAIAEDRGVKMPSFFGY
                     MLWSCGILVPLFIAMTFIWLR"
     gene            complement(9555..10754)
                     /gene="pncB"
                     /locus_tag="PK518_08730"
     CDS             complement(9555..10754)
                     /gene="pncB"
                     /locus_tag="PK518_08730"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRM49040.1"
                     /translation="MIITSLLDTDLYKFTMMQVVLHQFPGAQVEYRFKCRNPGVQLAP
                     HVDQIRDEIRALCTLRFQDAELAYLRSLRFIKSDFVDFLALFRLNEKYIQVSALPTGE
                     IDICITGPWLHTILFEIPVLAIVNEVYFRNTQKQPNFPEGRRRLDAKIAQLQADGLES
                     LKIADYGTRRRFSRAWHEEVLRVLCARLGTDDRGNGGAGQLAGTSNVLYAMQLGLTPL
                     GTMAHEYLQACQSLGPRLRDTQVFGFEMWAKEYRGDLGIALSDVYGMNAFLRDFDLYF
                     CKLFDGARHDSGDPFAWGERLLQHYRNHRVDPLTKTLIFSDALTVPRIIELHQRFHGR
                     CQLAFGIGTNLTNDLGHEPLQIVIKMTRCNDQPVAKLSDAPGKNMCDDEKYLAYLRQV
                     FDIPSPP"
     gene            complement(10884..11369)
                     /locus_tag="PK518_08735"
     CDS             complement(10884..11369)
                     /locus_tag="PK518_08735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRM49041.1"
                     /translation="MNASHVSSPSQAGRAPERPAPQARQTYPVLRPITTRWLDNDIYG
                     HVNNVVYYSWFDTAVNAYLIEQGALDIHQGETIGLVIETQCNYFAPLAFPQTIEAGIR
                     VARLGGSSVRYEVGLFAQGQELSAACGHFVHVYVGRGDRRPRPLPQRLRQVLEPLCLA
                     P"
     gene            11592..12146
                     /locus_tag="PK518_08740"
     CDS             11592..12146
                     /locus_tag="PK518_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRM49042.1"
                     /translation="MTMTPEQILASQKANIETLFGLTNKAFEGVEKLVQLNVAASRAA
                     LTEAAAHTQAVLSVKDAQELLALQAGLFQPMAEKAAAYSRHVYEIASGTNAEFGKAFE
                     SQTAEAQRSFSNLVDTAAKSAPAGSETTVAVFKSAVSAANNAFESVQKAVKQASDVAE
                     ANFNAVANSAANAAKTVEVAARKR"
     gene            12424..13473
                     /locus_tag="PK518_08745"
     CDS             12424..13473
                     /locus_tag="PK518_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM49043.1"
                     /translation="MSKPIKSLLVICALAAAGIASAQEQVVNLYSARHYPTDEALYSG
                     FTKATGIKIQRVDSDDAGIMARLKAEGSASPADVILLVDAARLYRGEADGLFLPVRSK
                     VLEDAIPANLRATPTADGGIPWFGLSTRARVIVYNKAKVNKDDVDTYEELGDPKNKGK
                     LCIRSGSHPYNLSLFGAVTEHMGEQKAEAWLKGVVANLARAPKGGDTDQIKAVAAGEC
                     DIAVSNSYYLARLMRSAKPEDKAVVDKVAVVFPNQQSWGTHLNIAGGAVARHTKNQAN
                     AIKFLEYLASPEAQNYFANGNNEWPAAKGVELDNPALKAMTDGKPFKSETIPIGAVGA
                     NTTKVQQMLDRVGFR"
     gene            13589..14347
                     /locus_tag="PK518_08750"
     CDS             13589..14347
                     /locus_tag="PK518_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRM49044.1"
                     /translation="MEIKGKVFIVTGGASGLGEGTARMLAAAGGLVVIADMQQDRGEA
                     VARDIGGAFVKCDVASEADGQAVVAKAVSMGKLMGLVNCAGIAPAEKTVGKNGSHGLA
                     SFSKTITVNLIGSFNMIRLAAEAMSKNEPEATGERGVLISTASVAAYDGQIGQAAYSA
                     SKGGVVGMTLPIARDLAKSGIRNMTIAPGIFGTPMLFGMPKEVQDALAAGVPFPSRLG
                     TPEDYAKLARHIFENDMLNGEVIRLDGAIRMAPK"
BASE COUNT         2547 a         4848 c         4716 g         2410 t
ORIGIN      
        1 gcacggtgcg cttggggcgg atggtgccgt agaactcgtt ctcgatctgc agcaggctgg
       61 tgcccagctg gttgtagtcg ccaccgatgt tgcgtatgcc gatcgcctca taggccgggt
      121 acggccgcgt gagcgcctcg tacagcgagt cggcatagcc cgacaggccg ttgtagctca
      181 cggccaggct ggcctgggcg tcgctctggt agcccaggcg gcccatgcgc agcgaggtgg
      241 catgcggcag gtgcagggcc tggctgccgt cgcccatggg ctgcaggctg tgcgcgcgcc
      301 cctcgacaaa gcaggggcac agcgcgggcg aggcgccaaa caggtagagc agcacgaagg
      361 cgtggcggcg gaagttgcgg atcagcgcaa agtattcctc gctcgtcacg cccggcagcg
      421 accagttgta gtggatgccc gaaatcgtct gcatgcgccg cccgtagcgg tgcgccaggc
      481 ccatgcggta gatgctcttg gcgcggccga tatgcgagtt gccatagcgg cctatgggga
      541 tggtctcgtc cgtgggcagg ccgcagggca tgctggagac ccagagcatc tcgccacccg
      601 cgccggccag cgtgcgcagc acatattggt ggatgtggat cagctcgtcc aggcagccag
      661 agacgctttg gtgcgcgcca gtgatgagct cgatctgcga ctcgctgtag tcggtggtga
      721 tgctggcgtg cgtcagggcc gagcccagcg cctgcgggtg cggcgtgagt gccagaccgc
      781 cctccggcag cacgcgcagc ccctcttttt ctatgccgcg gcgcataccc gctaggcgct
      841 cggccggcag ggctgcgatt cgttgttgcc aattgttcat tgtgtgctgt tgcttttttc
      901 tgcgaggggc cggaacttag cacgccgggc ccgcgcctgc ctgacaggac cgccaagcgc
      961 cacggcccag catgggcact agtacagcga cacggaggta tcggaacaag atgaaagtga
     1021 tggattcgcg cccagggcaa ggcgcaaacc gcagcgatag ccgtagctat cgcgaggatt
     1081 tgcaacgccg ccatgggggc gaaggcgcgc tttcatgttt cgatatttcc gtgttgctgt
     1141 acaaggccta cgcgcttacc ccggcctctg ccgccgccat ggccaggcgg atctcgttct
     1201 ggccctgggc ggcgccgctg gcgggcacct gctcaccgtg gcgctcgcag acctcggcca
     1261 ggcgggcggc cagttgttcg ggtacgtcgt cacccacgcc caggtacagg ccctgggtca
     1321 gggtgatatg cgcggcctcg aaggtgcccg cgccggcgca caggatggtg cgcgtgggcg
     1381 cgctctcgtg cgccagcacc agcatggccg gcaccacggc ctcgggccgg atggcggcca
     1441 tgacctcgtc gggcagcagg cccgcggtca tgcgcgtggc ggccgtgggc gccagcgcgt
     1501 tcacatggat gccgtacttg gcgccctcga tggccagggt ctgcatcagc cccgcctggg
     1561 ccagcttggc cgcgccgtag ttggcctggc caaagttgcc gtacaggccg gtggaggagg
     1621 tggtcatgac gatgcgccca tagttctgcg cctgcatcag cggccacacg gccttgcagc
     1681 aatgcgccgc gcccatcagg tgcacctgca ccaccagggc gaagtcggcc atgtccatct
     1741 tggcaaagct cttgtcacgc aggatgccgg cgttgttgac caggatgtcc acgcgccccc
     1801 aggcgtcctg cgcctgctgc accatggcct gcacggcggc aaagtcggtc accgaggcgc
     1861 cattggccag cgcctggccg ccggcggcgc ggatctcgtc ggccacggcc tgcgccgcgc
     1921 ccacgctgcc accgctgccg tccacggcgc cacccaggtc gttgaccagc accctggcgc
     1981 cgcgcgccgc cagcgccagc gcatgctggc gccccaggcc accaccggcg cccgtcacga
     2041 tggccacgcg gccttggaag tcgagggtca tagcaatcat ctccagagaa aggaaaagta
     2101 taaaaaccag agcaccctgc gcttgctaca caagcgctgg aggccttttt tactcacatt
     2161 cccgcggatc tgcacgatcc gcgccgcatg gcccggccgg gtcatgacct gcagataatg
     2221 acatgccggc cgcacagccc ccttgccatt cacccccacc gccatgaccg ataccgacac
     2281 cggcaccacg ctgctcgacc tgaacctgga ccgcaccctg gatctgtggc tgcgccgctt
     2341 ctcacagccg caatggcgcg gccggcatgt cgagggctgg ctgttcgagg gccaggccga
     2401 gcgccgcgcg gccgagcagc ggctggcggc cgtgggcgtg caggcgcgct ttcacagcgc
     2461 ctacaagccg ctggtgcagc atttcatcga caacgtcggc cgcgcgcagc tggtggcggc
     2521 ccatgtgcgc tacccggtgc cgcagatcgg cccagcgcag cgcttcaggc tggaggccta
     2581 cccgctcgcc gccctgctgc ccgaaggcgg gctgacgctg gagccacgcg ccgacgccct
     2641 gctgcactac gacgtcgagc ttgagctgca gggcggcaag cgcctgacgc aacgcgtgca
     2701 cgcacccaac cgccgccacg ccgacgccag cggcgccccg gcgctgtcgc ccacgggctg
     2761 gctgcgcgtg ggcaccatgg ccggcgcggc caacctgctg gactgcgccg tcagcaccga
     2821 ctacgagcag gcctttgccc aggcgctaca cgccgtgcgc gcccatccct ggccggatgc
     2881 ggagccttat ttcgagcgcc tgcagctgcg catcgacctg cccggctacg agcagcccat
     2941 tgcctgggcg ggcgagagca tcagcacggc cgaggcgctg cacgaggact tgtacttctc
     3001 gctgctcgaa ttcttccagc agcacagcgg ccgcccgctg ggcgaccggc ggctgcagcc
     3061 cggccagatc gtgcccgaca tacgcctggg cgccaccacg ccgcgcgtgc gggtcagcct
     3121 gcagcccttt gatgcgcagg cgcaggacgc gctggccgcc gcacaactgc cgcaggcggc
     3181 agcagcggcg ctggagagtc tgggcagcgc cccggcgccg gcctatatcg cccagatcct
     3241 gcagggctgg cccggccagc gctttgaggc gcccacgcgc cagggacgca gcgtgtgggg
     3301 ctattaccag gccggcacgc aacggccggt gttcatcagc ggtggccagc atgccaacga
     3361 gacctcgggc gtggtgggcg cgctgcgcgc cgccgacacg ctgcgccatg acggcagggc
     3421 ccatttcgcg ctcatcgcgc tggagaaccc cgacggctac gccctgcacc gcgagctgtg
     3481 ctgccagcac agcggccata tgcaccatgc ggcgcgctat accgccctgg gcgacgatgt
     3541 cgaataccgc gagggcgcgc cactgttcga gcgcgcggca cgccagcagg cgctggccct
     3601 gtcgggcgcg cagctgcatg tgaacctgca cggctacccg gcgcacgagt ggacgcgccc
     3661 gctgtcgggc tatgtgccgc gcggcttcga gcagtggatg tgtcccaagg gcttcttcct
     3721 gatcctgcgc taccacgccg gctggcagca gccggcgcgc acgctgctcg aggccgtgtg
     3781 cgcgcgcctc aaggcagtgc cgggcctggc cgaattcaat gcgcgccagc gcgctctgta
     3841 cgagcagcat gccggcccga tgccgttcga gctcatcgac ggcatcgcct gcacgcaggc
     3901 acagaccacg cacgacgcgc cagtgacgct ggtgaccgag ttccccgacg aaaccgtgca
     3961 tggcgacgcc ttccgctttg cacacctggt gcagatgcac accgtgctcg cggcggtgca
     4021 ggcctggcag cagatcgagc tgtgacgcgc gccggcctgc ctacaatgcc gcgatgctgc
     4081 acgctcttgc catctgcctc ggcgcctgcg ccggcgccct ggcgcgctgg cggctgtcgc
     4141 tatggctcaa cacccagggt gcgctgctgc cctggggcac gctggcggcg aacctgattg
     4201 gcggctatct catcggcctg tgtctggcgc tgttccaggc cctgccgcag ctcgaccctg
     4261 cctggcgcct gctgctcgtg accggctttc tcggcgccct gaccaccttt tccaccttct
     4321 cggccgaggt gctgggcctg ctgcagcagc agcgcctggc gctggccctg ggcctggcta
     4381 ccctgcatgt ggcgggctcg ctgtgcatga cctgggccgg actgcaaagc gcggcgctgc
     4441 tgcgccttga aagccggttt taaactatca tttacatagc tgttagcgct tgccatataa
     4501 gcgtttcaag ccaattaatc caacaatcaa accgcccact gacgctactc caccgctcaa
     4561 gccgacggcc ctttttccga taccaagaat catgcaagcc aacaccgacc ttcaagacag
     4621 ccgcctggcc aacgcctggg ccgagctgca caactacgcc gtggccggca atccgctgca
     4681 cgccgactcc tatcgcctgg cctttgccga ccccgaattc atgctgcgcc gcgagacacg
     4741 cggcatccgc tttcagctgg agctgctgaa gcccgagctg gaacaggccg accagggcat
     4801 agaacacacg gtggtggtgt atggcagcgc gcgctttgtt gcgcccgagg acgcggccga
     4861 gcagctggcc gaggcccagg ccagcggcga tgccgagcgc atcgcgcgcg ccgagcgcgc
     4921 cgtgaagaac gcctactact acgaccaggc gcgccgcttt gcgcgccatg tggccgaata
     4981 cagccagacg cgtccgccgg ccgagcgcct gtacatctgc accggcggcg gccccggcat
     5041 catggaggcg gccaaccgcg gcgcgcacga ggccggcgcg cccaacatcg gcctgaacat
     5101 cacgctgccg cacgagcaaa gcggcaacaa atacatcacg ccctcgctgt cgttcaagtt
     5161 ccactacttc gcgctgcgca agatgcattt catgatgcgc gccaaggccc tggtgctgtt
     5221 ccccggcggc tttggcacgc tggacgagct gttcgaggtg ctgaccctgg tgcagaccgg
     5281 caaggccaag gccgcgccca tcgtgctgtt cggctccgag ttctggaagc gcctgatcaa
     5341 cttcgacgcc ctggtggagc aaggcacgat ctcgcagggc gacctgaagc tgttccacta
     5401 ctgcgacgac ccggccgagg cctgggccat cgtgcgcgcg ttctacgagc tgtgagccca
     5461 gctggccgct gcccggtggc cgtgttccta atcgccttcg tcctctatca ataagtcctc
     5521 agcactcgcg ccggcatcgt ccagcgcctg ctgcccgggc gccggcagtt cctgcacgtc
     5581 gcgcaggcgc cgctgtatgg cgcgcgtgcg cacatcgacc tgctcgaact tcctggccgc
     5641 cgcgtcgatg gacttgcgcg tctgatccac gatgacgcca aacttggcga attcgctctt
     5701 cacctgcccc agcacgcccc agacctcgga cgagcgcttc tcgatcgcca gggtcttgaa
     5761 gcccatctgc aggctgctga gcatggccgc caggttggcc gggccggtga tcatcacgcg
     5821 gcagtcgttc tgcaccgcct ccaccaggcc ggggcggcgc atgacctcgg caaacaggcc
     5881 ctcggtgggc aggtagagca cggcgaagtc ggtggtgtag ggcggcgcca cgtatttgct
     5941 gaagattttc ttggcctcca gtttgatgga ggcctcgaag gcattgccgg cggcggcgat
     6001 cgcccccttg tcggcctggt cttgcgcgtc caccaggcgc tggtaatgct ccacggggta
     6061 cttggagtcg atcggcagcc acaccggctg gtcgtcgctg cggcccggca ggcggatggc
     6121 aaattccacc agctcgtcgc tgccgggctg ggtcttgacg tttttctcgt actgcccggg
     6181 cgtgagcacg ttgtcgatga tggcgcccag ctgcagctcg ccccaggtgc cgcgcgtctt
     6241 cacgttggtc atcacgcgct tcaggtcgcc cacgccggcg gccagggtct gcatttcgcc
     6301 caggcccttg tgcaccagct ccaggcgctc gctgaccagc ttgaaggagt cgcccaggcg
     6361 ctgctccagc gtggcgtgca gcttctcgtc cacggtgcgg cgcatttcct ccagcttggc
     6421 ggcgttgtcc tgctggatgg cggccaggcg ttcgttgagc gcagcgcgca gcgcctcggc
     6481 gccctgctgg ctgctggccg ccaggcccgt gagctgctgg cccaccgcct cctgcaggcc
     6541 ggcgaactgg tggcgcagct gctgcgtatg cgcctccagc tgcgcctgca catcgccctt
     6601 caaggcctgg gcggtctgcg tgagctcggc gcggaaggcg gccagcgcct gcagctgctc
     6661 atggcgcgcc tgggcctcat cgctgcggcc ctgcaccagt tgctgctcca gccgctgctg
     6721 cagcgcgcca aaggctgcct gcagctcggc gcggctggtg cgcgactcct gcgccgccag
     6781 ctgcaggcgc tcgtccagta gcacctgcgt gctgtgcgtg aagccgcgca gctgctggcc
     6841 catggcgtcc agcgcgccat cgttcttggc caccgccagt tgcgtggcct gcactgccga
     6901 ctccagcgcc tgcaggcgct gcggccactc gggcggcagc gcaacacggg ggcggcgcag
     6961 taacagggcg cacagcagca gcacgaccag caccagcagg gccagcgcga tcatcatttc
     7021 aagggtcatg gaatactatc gattcaatag ctgtttgcgc tttgtggata agcgctacag
     7081 cctgtttttg cttattactg attgggggta ttcaccggcg tcagcgcgcc gcgcccgtca
     7141 aagaaggcca ccaggttgtc cgccgccagc cgcgccatgg ccaggcgcgt gggtacggtg
     7201 gcactggcga tatgcggggt gagcaccacg ttggagcgct ccagcaggcc ggggtgtacg
     7261 gcgggctcgc cctcgaacac atccaggccg gcggcggcga tgcgcccctc cttcagggcc
     7321 tgggccagcg ccgcgtcatc gacgatgccg ccgcgcgcaa tattcaccag cgtggccgtg
     7381 ggcttcatgc gcgcgagctc tgccgcacca atggcgtggt gcgactcggc gctgtagggc
     7441 agcaccagca ccaggtggtc ggcctgggcc agcaactcgt ccttgctgac atagcgcgcc
     7501 ttgcattcgg cttccagctc gggcgccagg cgcgagcggt tgtggtagat cacctgcatg
     7561 ccaaagccat gcgcgccgcg ccgggcaatg ccctggccga tgcggcccat gcccagaatg
     7621 cccagggtgc tgccatgcac ctcggcgccg gcgaacatgt cgtagcgcca gctgttccac
     7681 aggccggcgc gcaggtaatg ctcggactct gtcatgcgcc gcgccgtggc catgagcagt
     7741 gcaaaaccga aatcggccgt ggtttcggtc agcacgtcgg gcgtgttcgt gccctgcacg
     7801 ccggcggcgg tcatggcctg cacgtcgaaa ttgttgtagc ccacggccat gttggcggcg
     7861 attttcagac gcgggcaggc ggccagcagg gccgcgtcta tgcggtggct gccggtgatg
     7921 aagacgccgt ccttgtcggc cagacgctgg gcgagttctg cctggctcca cagcacatca
     7981 tcggggttgg attcgagctc gaaatgctgc gccaggtagt cagtgatctc gggaaagata
     8041 tggcgtgtga ccaggatgcg gggcttggac atggggcgtt tcctttctgt agtcagcgca
     8101 gccagatgaa ggtcatggcg atgaacagcg gcaccaggat gccgcaggac cagagcatgt
     8161 agccgaagaa gctgggcatc ttcacgcccc tatcctcggc aatggccttg accatgagat
     8221 tgggcgcgtt gccgatatag gtgttggcgc ccatgaacac cgagccggcg gagatggccg
     8281 ccagagtcgt ggcgtaggtg gtcatcagcg tggccgggtc gccgccggcg gtgttgaaga
     8341 acaccagata ggtgggcgcg ttgtccagga aggaggacag caacccggtg gcccagaagt
     8401 acatggccgg gtcgggctgg ccatcggccc gcgtgaccgc ggaaacgatg gcgccaaacg
     8461 gcccgttgac acccgccttg agcatggcga tgaccgggat gatggtcaga aagataccgg
     8521 caaacagctt ggccacctcc tgcatcgggc cccagccgaa ctggttgtcg ctgtgcacct
     8581 ggcgcggcgt gagcaccagc gacaccagcg tgatggcgat cagccccagg tcgcgcagca
     8641 ggccgggcag gcccaccggg gtgccggcta tcgtccattg cacgctggac ttccacatgc
     8701 cgctcatcag caccagggcc accacgccca ggagcagcac gaagttgatc ttgccgtcaa
     8761 agcccagcgc ggcgccgcct gagtcgggcg tggggtcggt acgccgcagc tcgccgtcct
     8821 ggcggaagta ccaggagtca atggcaaaga acagcaccag cagcacggcg atcaggaaca
     8881 gcgtctcggg gaagatgtgg cgcagcgtcc agaaaaaatc cacacccttc agaaagccca
     8941 ggaacaaggg cggatcgccc agtggcgtga gcgagccgcc cgcgttggag acgacgaaga
     9001 tgaagaacac caccacatgg gccacatgcc tgcggttgtc attggcgcgt atcaacgggc
     9061 gtatcagcag catggatgcc cctgtggtgc ccatgaagct ggccagcacc gcgcccacca
     9121 acaggatgcc ggtgttgagc accgggctgc cgcgcaggtt gccgcgtatg tagatgccac
     9181 cggcgacggt gaacagcgcc gacagcagga tgacgaaggg caggtattcg gccgccaggg
     9241 catgcacaaa ctgggcgccc gccgcctgcg gcccaaagac gaccgcaaat ggcagcagaa
     9301 atgccagtga ccaacccacc accagcttgc cgaaatggtg gtgccagaac agcggcgcca
     9361 ccatgggcag gagcgcgatc gacagcagga tgccggcaaa gggcacgccc cacagcagcg
     9421 acagctggcc gccatcgatg tccgcggcag cggccagggc cggcgccagg ctgagggcgg
     9481 tcagggtcag ggtgcgaaaa agcttcatgg gacagtctct cctgggttct tgttctgatg
     9541 ctgcgcagca gcgctcatgg cgggctggga atgtcgaaca cctggcgcag gtaggccagg
     9601 tatttctcgt cgtcgcacat gttcttgccc ggcgcgtccg acagcttggc caccggctgg
     9661 tcgttgcagc gcgtcatctt gatgacgatc tgcagcggct catggcccag atcattggtc
     9721 aggttggtgc caatgccaaa ggccagctgg cagcggccat gaaagcgctg gtgcagctcg
     9781 atgatgcgcg gcacggtgag cgcgtcgctg aagatcagcg tcttggtcag cgggtcaacg
     9841 cggtggttgc ggtaatgctg caagagccgt tcgccccagg caaaggggtc gccgctgtcg
     9901 tggcgtgcac cgtcgaacag cttgcagaag tacaggtcga agtcgcgcaa aaaggcgttc
     9961 atgccgtata catcggacag tgcaatgccc aggtcaccgc ggtactcctt ggcccacatc
    10021 tcgaagccga acacctgggt atcgcgcagg cgcgggccca gcgactggca ggcctgcagg
    10081 tattcgtgcg ccatggtgcc cagcggtgtc aggcccagct gcatcgcata cagcacattg
    10141 ctggtgccgg ccaactgccc ggcgccgccg ttgccacggt cgtcggtgcc caggcgtgcg
    10201 cacagcacgc gcagcacctc ctcatgccag gcgcgtgaaa agcgccggcg ggtgccatag
    10261 tcggcaatct tcaggctctc caggccgtcg gcctgcagct gcgcgatctt ggcgtccagc
    10321 cgcctgcgcc cctcggggaa gttgggctgc ttttgcgtgt tgcgaaagta cacctcgttg
    10381 acgatggcca gcaccgggat ctcgaacagg atggtgtgca gccaggggcc ggtgatgcaa
    10441 atgtcgatct cgcctgtggg cagggcgctg acctgtatgt acttttcgtt cagccgaaac
    10501 agcgccagaa agtccacgaa atcgctcttg atgaagcgca gcgagcgcag ataggccagc
    10561 tccgcatcct gaaagcgcag ggtgcacagg gcgcggatct cgtcgcgaat ctgatccaca
    10621 tggggcgcca gctgcacgcc cgggttgcgg catttgaagc ggtattcgac ctgcgcgccc
    10681 ggaaactggt gcagcaccac ctgcatcatg gtgaacttgt acaggtcggt gtcgagcagg
    10741 ctggtgatga tcatggatgc gcgcgccagc cctgtgggcc ggtgctggtc aggggcggcg
    10801 acgggcaccg cagggtgttt gtctctgcgg tgattctggc atcaatgtct ggcacccatg
    10861 gcaataccca ttgccaccct gccctatggc gccaggcaca ggggttcaag cacctggcgc
    10921 agccgctggg gcaaggggcg cggacggcga tcaccccgcc ccacatagac atgcacgaaa
    10981 tggccgcagg cggcgctcaa ctcctggccc tgggcgaaca ggccgacctc atagcgcacg
    11041 ctgctgccgc ccagccgcgc cacgcggatg ccggcctcaa tcgtctgcgg aaaggccagc
    11101 ggcgcaaagt agttgcactg ggtctcgatc accaggccta tggtctcgcc ctggtgaatg
    11161 tccagcgcgc cctgctctat cagataggca ttcacggcgg tgtcgaacca actgtagtac
    11221 acgacattgt tcacatggcc atagatgtcg ttgtccagcc agcgggtcgt gatcggccgc
    11281 agcacggggt aggtttgtct ggcctgcggc gcggggcgct caggggcgcg accggcctgc
    11341 gaaggggaag aaacatgcga cgcattcatg gaagggtgat tctgccagcc aggcgccgcg
    11401 catcggcgca gccgtaacca acggaacatt ggggcaatga ccctaaaaat gttgcaccgc
    11461 aacaaaaaag acttgcaagt ctcgccagga tgcctaaaat tcgtcccatg ctgcactgca
    11521 acatgactca acggtcagtc agggcagacc tcttaacccg tccgcaaggg catttcaatt
    11581 ggagtaatga catgaccatg acccccgagc aaatcctcgc ttcccaaaaa gccaacatcg
    11641 aaaccctgtt cggcctgacc aacaaggcct tcgaaggcgt ggaaaagctg gtgcaactga
    11701 acgttgccgc ctcgcgcgcc gccctgacgg aagccgctgc ccacacccag gccgtgctgt
    11761 cggtcaagga cgcacaagag ctgctggccc tgcaagccgg cctgttccag cccatggctg
    11821 agaaggctgc cgcttacagc cgccacgtgt acgaaatcgc ctccggcacc aacgccgagt
    11881 tcggcaaggc ctttgaaagc cagaccgcag aagcccagcg tagcttcagc aacctggtgg
    11941 acaccgctgc caagagcgca cccgccggtt ccgaaaccac cgttgccgtg ttcaagagcg
    12001 ccgtctccgc cgccaacaac gctttcgagt ccgtgcaaaa ggccgtgaag caagcgagcg
    12061 acgtggccga agccaacttc aacgccgttg ccaactcggc cgccaacgct gccaagacgg
    12121 ttgaagtcgc tgcccgcaag cgctgatagc ccggctgcgc acatgcccat gtgcgcagtt
    12181 cagggttaac acctaaaatc tcaacttcga tcttgttgtt ctttttaata acaaggttct
    12241 ccagttgtct ccttggatcc tctcgaaact ccgcgtttct gggatccctt caagcccggt
    12301 cttctgactg ggcttttttt tgcgtgctcg atgatggccc gctggaatca cggtttattt
    12361 aagaacgatt ctcgtttgcc ttatgatccc ggcatcacat caccgagaag gaactgatac
    12421 cccatgtcca agcccatcaa gtcactgttg gtcatctgtg cactcgctgc agccggcatc
    12481 gccagcgccc aggagcaggt cgtcaacctg tattcggcac gccactatcc gacggacgag
    12541 gccttgtaca gcggctttac caaggccacg gggatcaaga tccagcgcgt ggactcggac
    12601 gacgccggca tcatggcccg cctgaaggcc gagggctcgg cttcgcccgc ggacgtgatc
    12661 ctgctggtgg acgccgcccg cctgtaccgc ggcgaggccg acggcctgtt tctgccggtg
    12721 cgctccaagg tgctcgagga cgccattccc gccaacctgc gcgcaacccc cacagccgac
    12781 ggcggcatcc cctggttcgg cctgtccacc cgtgcccgcg tcatcgtcta caacaaggcc
    12841 aaggtcaaca aggacgacgt agacacctac gaggagctcg gcgaccccaa gaacaagggc
    12901 aagctgtgca ttcgctcggg ctcgcacccc tacaacctga gcctgttcgg cgccgtgacc
    12961 gaacacatgg gcgaacaaaa ggccgaggcc tggctcaagg gcgtggtcgc caacctggcc
    13021 cgcgcgccca agggaggcga caccgatcag atcaaggccg ttgccgccgg cgagtgcgat
    13081 atcgccgtca gcaacagcta ctacctggca cgcctgatgc gctcggccaa gcccgaggac
    13141 aaggccgtcg tggacaaggt ggccgtggtc ttcccgaacc agcagtcctg gggcacccac
    13201 ctgaacatcg ccggcggcgc cgtggcgcgc cacaccaaga accaggcgaa tgccatcaag
    13261 ttcctggagt acctggccag cccggaggcg caaaactatt ttgccaacgg caacaacgaa
    13321 tggcccgcag ccaagggcgt ggaactggac aacccggcgc tgaaagccat gaccgacggc
    13381 aagcctttca agagcgagac cattcccatc ggggccgtgg gcgccaacac caccaaggtg
    13441 cagcagatgc tcgatcgcgt cggtttccgc tgatccgctc cggggcaagc cttcaccgaa
    13501 cccggcccgg ccgggttttt ttcatccgat gaattgcgac gacgtcataa ttgacgtgta
    13561 cgtcaacgtt atttgcagga gaccaggaat ggaaatcaag ggcaaggtat tcatcgtcac
    13621 gggcggcgca tccggcctgg gcgaaggcac ggcgcgcatg ctggccgccg ccggcgggct
    13681 ggtggtgatt gccgacatgc agcaagacag gggtgaggcc gtggcacgcg atattggcgg
    13741 cgccttcgtc aagtgcgatg tggccagcga ggccgacggc caggcggtgg tcgccaaggc
    13801 cgtctctatg ggcaagctca tgggcctggt gaactgcgcc ggcatcgccc cggcggaaaa
    13861 aaccgtgggc aagaacggct cgcacggcct ggccagcttc agcaagacca tcaccgtgaa
    13921 cctgatcggc agcttcaaca tgatccgcct ggcggccgag gccatgagca aaaacgagcc
    13981 cgaggccacc ggcgagcgag gcgtgctgat ctccaccgcc agcgtggcag cctacgacgg
    14041 gcaaattggc caggcggcct attccgcatc caagggtggc gtggtcggca tgacgctgcc
    14101 gattgcgcgc gacctggcca agagcggcat acgcaacatg acgatcgcgc cgggcatctt
    14161 cggcacgccc atgctgttcg gtatgcctaa ggaagtgcag gacgcgctgg ctgccggcgt
    14221 acccttcccc agccgcctgg gcacaccgga agactacgcc aagctggcgc gtcacatttt
    14281 tgaaaacgac atgctcaacg gcgaggtgat ccgccttgat ggcgccatac gcatggcacc
    14341 gaagtgatgt atttttaaga gctgcaaacg cttgtcacgc ctgccctgca ggcgtttttc
    14401 ttttgaaacg aagcagcagc aaaagcaaac gggctgcaac ttgcgatgca gcccgttttg
    14461 agagacttgg cggagtggac gggactcgaa cccgcgaccc ccggcgtgac aggccggtat
    14521 t
//
LOCUS       DAOMTQ010000074        14216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_696_length_14216_cov_30.029094, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000074 DAOMTQ010000000
VERSION     DAOMTQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14216
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_14216_cov_30.029094"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK518_08755"
     CDS             <1..521
                     /locus_tag="PK518_08755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRM49045.1"
                     /translation="LYENGEFYFIEMNTRVQVEHPVTEWITGVDIVRTQIMVAAGEKL
                     PFTQRQIQIQGHAIECRVNAEDPYRFIPSPGRITMWHAPGGPGVRVDSHAYTNYYVPP
                     NYDSMIGKIIVHGDTREQALARMRTALSETVIEGINTNVDLHRELLVDAKFVAGGTNI
                     HYLEEWLAQRKR"
     gene            609..1505
                     /gene="prmA"
                     /locus_tag="PK518_08760"
     CDS             609..1505
                     /gene="prmA"
                     /locus_tag="PK518_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRM49046.1"
                     /translation="MFELSLLCPEDRVETLSEAFDALEALSVSVEDADAQTDAEQALF
                     GEPGMPAPREGWQRSRITALFATRQAADEAVQLLQVQDFFAGCSVLGIAAVQEQDWVR
                     LTQSQFEPVEITPDFWIVPTWHELPAAALRSIRLDPGLAFGTGTHPTTRMCLRWIARQ
                     GGADGARALGRVLDYGCGSGILAMGAAKFGASDIDAVDIDAAAVEATRQNAQANGVQL
                     NAGLPDQARGEYQTVLANILATPLKVLAPLLCAHVARGGHLVLAGILERQAEELQQAY
                     APWLPLQVADSEDGWILMTARR"
     gene            1563..2897
                     /locus_tag="PK518_08765"
     CDS             1563..2897
                     /locus_tag="PK518_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRM49047.1"
                     /translation="MSQITRCPHCATAFKVVADQLRISDGWVRCGQCKEVFDASEHLL
                     MDESEPLLPEMPLHGLHAPQQSGALTPDSVHVWGSARGTEPSATPEGPPADLAPPQAA
                     EGQELDQPSVMTGAVPEGADTPRYAEVRAYAGTPAHADDTHEPIVLSSLLRQESSAQA
                     LMAPAAPLQEIQGYELPGAQESDSGWPEDTAAEAPVAPEAGAEGVSTPLPQEPTAAAS
                     ENSDEQPAQAEQPDLQVLEPVQPEPLLAEEAALMPTVALADSDPAHAETAALPEEPGF
                     VATARRKAFWRRPVVRAALVMLGLVFLLALSLQMTLQERELIAARNPAMRTLLEQLCQ
                     PLGCTLDAPQRIEAVVIDSSSFLKARHDDASYELQMAIKNNATFAVAMPALELTLTDA
                     QDQTLLRRVLLRDELGAPAQLAPGATWSASVPMQVMQGAAQVAGYRLLAFYP"
     gene            2925..3821
                     /locus_tag="PK518_08770"
     CDS             2925..3821
                     /locus_tag="PK518_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRM49048.1"
                     /translation="MAALICGSLAFDSIMTFEGRFAEQILPAQLHILNVSFLVPTLRR
                     DFGGCAGNIAYSLKLLGGDPQPMAMLGADGGEYLARLQQLGIDTRYVGQAGDTYTAQA
                     MIMTDRDNNQITAFHPGAMMQAHTNRIGPDAGARIGIIAPDGRDAMLQHAAQFVEAGI
                     PFVFDPGQGLPMFDGDELRRFVAQAGWIAVNDYEGKMLCDRTGWSLAELSQQVTGLVV
                     TLGAEGCEVWEQGARTKVDPVTPAQVVDPTGCGDAWRGGLLYGLEKGWPLVRCAELGN
                     RLGALKIAQRGPQNYQLDFTPD"
     gene            complement(3873..4484)
                     /locus_tag="PK518_08775"
     CDS             complement(3873..4484)
                     /locus_tag="PK518_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HRM49049.1"
                     /translation="MATAKKPAAKKAAPAKKAAAPAKKAAAPAKKAVVAKKAAAPAKK
                     AAAPAKKAAAPAKKAVVAKKATAPAKKAAAPAKKAAAPAKKAVVAKKAAAPAKKVAAP
                     AKKAAAPAKKAVVAKKAAAPAKKAAAPAKKAAAPAKKAVVAKKAAAPAKKAAAPAKKA
                     PAVKKAPAAKKAAAKAPAAAPAPAAQTTLSPQAAWPFPTGGKP"
     gene            complement(4662..5846)
                     /locus_tag="PK518_08780"
     CDS             complement(4662..5846)
                     /locus_tag="PK518_08780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRM49050.1"
                     /translation="MLTWDDEVKPSSQNLFDGALASSRAAEPSPTALAAQTLQRATGT
                     MAHVPTAAAAQHRRVSAVDKRIINGQTDVNQLVPFKYKWAWEKYLATCANHWMPQEIN
                     MTRDIALWKDPNGLTEDERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQFLL
                     RQAFEEAIHTHAYQYIVESLGLDEGEIFNAYNEIQSIREKDEFLIPFIEAIMDPGFKT
                     GSPENDQTLLKSLIVFACLMEGLFFYVGFTQILALGRQNKMTGAAEQYQYILRDESMH
                     CNFGIDLINQLKLENPQLWTAAFKEEITALFHKAVDLEYRYAEDTMPRGVLGMNASMF
                     KGYLRYIANRRATQIGLEALFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALS
                     WD"
     gene            complement(5974..8850)
                     /locus_tag="PK518_08785"
     CDS             complement(5974..8850)
                     /locus_tag="PK518_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRM49051.1"
                     /translation="MQTASVHADSFSQLQIDAADTSTPASMADTLSHYQIIRRNGAVV
                     HFEPQKIAVAMMKAFLAVHGTQGAASASVRQVVDELTQTVVRALMRSRPSGGTFHIED
                     VQDQVELGLMRGSHHEVARAYVLYRERRAQERARKKEQLHPQIPALYVQDRGQRVELD
                     LLRLQALIESACANLGTDVEAAPIAAETLRNLYDGVPMEEVYKASILAARTLIEKDPG
                     YSYATARLLLHTIVREVLGRGVQPGEMQAAYMDHFPAFVQKGVDNELLNPDLLSFDLP
                     RLAAALRAERDQQFDYLGLQTLYDRYFLHVNKARIELPQSFFMRVAMGLALREDDRNA
                     RAIEFYELLSSFDFMSSTPTLFNSGTLRSQLSSCYLTTVPDELGGIYESIKENALLSK
                     FAGGLGNDWTRVRALGAHIKGTNGESQGVVPFLKVVNDTAVAVNQGGKRKGAVCTYLE
                     SWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMGKGQWTLFSPSDVPDLH
                     DLYGQAFESAYAAYEAKTESGELKLFKRLPAVDLWRKMLSMLFETGHPWITFKDPCNI
                     RSPQQHVGVVHSSNLCTEITLNTSDSETAVCNLGSVNLAQHLKDGAIDHDKLRRTVTT
                     AMRMLDNVIDINYYAVDKARDSNLRHRPVGLGLMGFQDALYELRMPYASDAAMEFADA
                     SMEAICYYAYWASTELARERGSYSTFQGSLWDRGVLPLDSLGLLAQERGGHVEVDTST
                     RLDWDALRQKIARDGMRNSNCVAIAPTATISNIVGVDASIEPSFGNLSVKSNLSGEFT
                     VINQYLVRDLKTLGLWDDVMLMDLKHFKGSLHAIDRVPADVKALYSTAFEVAPTWLVE
                     AAARRQKWIDQAQSLNIYMAGASGKKLDETYKLAWLRGLKTTYYLRTQSATHVEMSTV
                     NTRQLNAVSSGADHPSRTLDADAAIPATDVKFCAIDDPGCEACQ"
     gene            complement(9140..9730)
                     /gene="ampD"
                     /locus_tag="PK518_08790"
     CDS             complement(9140..9730)
                     /gene="ampD"
                     /locus_tag="PK518_08790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HRM49052.1"
                     /translation="MAPGNRPQWRAGWYLPARRLPSPNHGARPAGAVVDLIVVHSISL
                     PPGEYGGNQVQELFTNRLDWDAHPYFQQIHGLEVSSHFFITRLGQLWQFVATDRRAWH
                     AGRSHYRGRDGCNDDSIGIELEGLEGHTFEEAQYAALTRLCAALVRHHPIGYVAGHEH
                     IAPGRKNDPGPGFDWRHLRSAFAGSGILFPPAAQPA"
     gene            complement(9714..11195)
                     /locus_tag="PK518_08795"
     CDS             complement(9714..11195)
                     /locus_tag="PK518_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRM49053.1"
                     /translation="MNATAATILVVDDEPDLRTLYELTLLREGYRVQTAGSVGEAREQ
                     LQNQTFDAVITDMRLPDGLGMELLHELRAQQRGERCVVMTAYGSAENAVEALRSGAFD
                     YLTKPVDLKQFRSVVASAIQGAAGVLASAKPGRSTAPGKGDDNPVLARLIGESEAMRG
                     VKQRIAKVARSMAPVLIHGESGTGKELVARALHASSQRAGGPLVAVNCGAIPENLLEA
                     EFFGARKGSYTGAASDREGYFQAARGGTLFLDEIGDLPLAMQSKLLRAIQERSVRPLG
                     STQEETVDVRIVSATHRDLAADVQAGRFRQDLYYRLNVIELLIPPLRERREDLPALCH
                     ALLERIQQESGIPVPQLSEAALQRIAQHPLTGNVRELENLLHRAVALSDGHELHVDGP
                     TQASSAGDAAPPPPPPPPPPAAKLVAQAPTPEPTTAPPAGDLQAWLDQRERGLLMQTL
                     HETSFDPVATAARLGVSQRQMRYRIERLNIAIPNPGDSHGAGQ"
     gene            complement(11209..12897)
                     /locus_tag="PK518_08800"
     CDS             complement(11209..12897)
                     /locus_tag="PK518_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM49054.1"
                     /translation="MPALQASHPSFQRLWKAFLTARLMVALALLLLQLLAMALNQTGV
                     LAPLGVALGYLLATSLLLVFGARMPPDPRPGLHWLPSIGVDLVLIALLQMLHQSGSMN
                     FTPLFGLPILMAAVLGTLTLALGTTAGATLLLLAWAWWLGDQGGGDAAQRYLQTALTG
                     TGYFFIAYLVHQLAVRLSREQLVARRSQMNARTQKAVSALVMQHVADGVLVLNRQGQV
                     RLANPAALLLLGPDARTSTPFMLTHTQAWSPLMELAQASFEQAQPQHADVNILHEGHS
                     PMGLRIRTWLTHAPGALGAVDEEPLCVMFLHDLREMEARLRTEKLASMGRMSAAVAHE
                     IRNPLAAIVQANELLAEDLQEPGQKRLSHMIGQNADRLVRITEDVLDIARVQHQIGQT
                     PSSTLALNATVAQTWHDWQAHDPAGRQGQLLLGAGEVPVEFDPEHLRRVLVNLLDNAQ
                     RYKGPYADSLQLSTRATASGQIGIQVWSDGAPLDKSVERHLFEPFFSSESRSSGLGLY
                     ICRQLCQRHGAAISYQRSTRMLDRGTVMGNAFTVSFRSATRPFETDSLFDIIVV"
     gene            complement(12919..13146)
                     /locus_tag="PK518_08805"
     CDS             complement(12919..13146)
                     /locus_tag="PK518_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HRM49055.1"
                     /translation="MKYLIVLAVIAVVYALWRSQRQVPPNRGSAPRQNPAQPQDMVCC
                     AHCGVHLPKGEALHQGGHSYCSRAHQDLGPP"
     gene            complement(13143..13940)
                     /gene="ccsA"
                     /locus_tag="PK518_08810"
     CDS             complement(13143..13940)
                     /gene="ccsA"
                     /locus_tag="PK518_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRM49056.1"
                     /translation="MILPSASPSTWLLALVTAAAYAIGAAVASRSQASRSHLAMAPAW
                     ALHAGAIGWSQLGGAPHFGFAAALSMTAWLVLTIYALERELFPQMRTRWALGALGGVA
                     VLLAALFPGRPLQATASPWMPLHLTLGIACYGLFAAAVAHAWLMSRAERQMRQATQLH
                     SSLPLLTLERLTFRFVRWGFALLSATLVAGWLFSEQLYGRAWLWDHKAVFSVLAWVTF
                     AVLLLGRARFGWRGRNAVRVLYAGAMLLLLAYVGSRFVLEVVLGRTT"
     gene            14035..>14216
                     /locus_tag="PK518_08815"
     CDS             14035..>14216
                     /locus_tag="PK518_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRM49057.1"
                     /translation="MASALTDKLTRLVKEMRGQARITESNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKDKALG"
BASE COUNT         2303 a         4520 c         4826 g         2567 t
ORIGIN      
        1 ttctgtacga aaacggcgag ttctacttca tcgagatgaa cacccgcgtg caggtcgagc
       61 acccggtcac cgagtggatc accggcgtgg acatcgtgcg cacccagatc atggtggcgg
      121 ccggcgagaa gctgcccttc acccagcgcc agatccagat ccagggccac gccatcgaat
      181 gccgcgtgaa tgccgaggat ccataccggt tcattccctc gccgggccgc atcaccatgt
      241 ggcatgcacc gggtggcccg ggtgtgcgcg tggactcgca tgcctacacc aactactacg
      301 tgccgccgaa ttacgactcc atgatcggca agatcatcgt gcatggcgac acgcgggagc
      361 aggccctggc gcgcatgcgc acggcgctgt ccgagacggt gatcgagggc atcaacacca
      421 atgtggatct gcaccgcgag ctgctggtgg acgccaagtt cgtcgccggc ggcaccaaca
      481 tccactacct tgaggagtgg ctggcgcagc gcaagcgctg atggcccgcc ccattcacgg
      541 caatgccggc ttctggcgac agatgccggc attttttttc ttgattgcac gacgcgaagg
      601 tgtgccccat gtttgaactg agcctgctat gccccgaaga ccgggtggag accctgagcg
      661 aggcctttga tgcgctggag gcgctgagcg tgtcggtgga ggacgccgac gcccagaccg
      721 atgccgagca ggcgctgttc ggcgagcccg gcatgcccgc gccgcgcgag ggctggcagc
      781 gcagccgcat cacggcgttg tttgccaccc ggcaggcggc cgatgaggcc gtgcagctgc
      841 tgcaggtgca ggatttcttc gccggctgca gcgtgctggg tatcgcagcc gtgcaggagc
      901 aggactgggt gcggctgacg cagtcgcagt tcgagccggt ggagatcacg ccggacttct
      961 ggatcgtccc gacctggcac gagctgcccg cagccgcact gcgcagcatt cggctggatc
     1021 cgggcttggc cttcggcacc ggcacccatc ccacgacgcg catgtgcctg cgctggattg
     1081 cgcgccaggg gggtgctgat ggagcgcggg cgctcgggcg cgtgctcgac tatggctgtg
     1141 gctccggcat tctggccatg ggtgctgcca agtttggcgc ctccgacatc gatgccgtgg
     1201 acattgatgc cgcggccgtg gaagccacgc gccagaacgc ccaggccaat ggcgtgcagc
     1261 tgaacgccgg cctgccggat caggcgcggg gcgaatatca aacggtactg gccaatattc
     1321 ttgccacgcc gctgaaggtg ctggcgccct tgttgtgcgc gcatgtggcg cgtggcgggc
     1381 atttggtgct ggcgggcatt ctggaacgcc aggccgagga gctgcagcag gcttacgcac
     1441 catggctgcc gctgcaggtg gcagacagcg aggacggctg gattctgatg accgcgcggc
     1501 gctgagcggc aggcgcgcgc gcctcgcgtg ggcctctggt cgccacctac aatccacagc
     1561 cgatgagcca gatcacccgc tgcccccact gcgcaacagc cttcaaggtg gttgccgatc
     1621 agctgcggat atctgacggc tgggtgcgct gcgggcaatg caaggaggtg ttcgacgcgt
     1681 ccgagcattt gctgatggac gaatccgaac ccctgttgcc cgagatgccg ctgcacggcc
     1741 tgcatgcacc ccagcagagc ggcgccctaa cgccggacag cgttcatgtc tggggcagtg
     1801 cccgagggac tgaaccatcg gccacgcccg aagggccgcc cgcggatctg gcgccaccgc
     1861 aggcagcgga agggcaggaa cttgatcaac cgtctgtcat gacaggcgcc gtaccggagg
     1921 gtgcggacac gccgcggtac gccgaggtgc gggcctatgc cggtacgccc gcccatgctg
     1981 acgacacaca tgaacccatt gtgctgtcgt ccctgctcag gcaggaatcg tcggcgcaag
     2041 ctctgatggc acctgcagca ccgctgcagg agatccaggg ttatgagctg cccggtgcgc
     2101 aggagagtga ttccgggtgg ccggaggaca ccgccgctga ggcacccgtg gcgcccgagg
     2161 ccggtgcaga gggcgtgtcc acgccgttgc cgcaggagcc caccgcagcg gcttccgaga
     2221 acagtgatga gcagcctgca caagctgaac agcccgatct gcaggtgctg gagcctgtgc
     2281 agcccgagcc cctgctggcc gaagaggcgg cgctgatgcc gaccgtcgct cttgccgact
     2341 ccgatcccgc gcacgccgag acagcagcat tgcccgaaga acccggcttt gttgcgacgg
     2401 cgcggcgcaa ggcgttctgg cgccggcccg tggtgcgagc tgccttggtg atgctggggt
     2461 tggtattttt gctggcgctg agcctgcaga tgacgctgca agagcgcgaa ctgatcgctg
     2521 cacgcaaccc ggcaatgcgc accttgctcg agcagctgtg ccagcccctg gggtgcaccc
     2581 tggacgcgcc acagcgcatc gaggccgtgg tgatcgacag ctcgtccttt ctgaaggcgc
     2641 gccacgacga tgcgagctac gagttgcaaa tggccatcaa gaacaatgcg acgtttgcag
     2701 tggccatgcc cgcactggag ctgacattga ccgatgcaca ggatcagacc ttgctgcgtc
     2761 gtgtgttgct gcgtgacgaa ttgggcgctc cagcccagtt ggcgcccggc gccacatgga
     2821 gtgcctctgt gcccatgcag gtcatgcagg gtgcggcgca ggtggccggt tatcgcttgc
     2881 tggcctttta cccctgagtt tttttacttc ccacctctga ctctatggct gccctgatct
     2941 gcggttccct ggcattcgac agcatcatga ccttcgaggg ccgctttgcc gagcaaatat
     3001 tgcccgcgca attgcatatc ctgaacgtgt ccttccttgt gcccacactg cgccgtgact
     3061 ttggtggctg tgccggcaac attgcctaca gcctgaagct gctgggcgga gatccccagc
     3121 ccatggccat gctgggcgcc gatggcggcg agtatctggc gcgcctgcaa cagctgggca
     3181 tagatacccg ctacgttggc caggcaggcg acacctatac ggcgcaggcc atgatcatga
     3241 ccgaccgtga caacaaccag atcacggcct tccatccggg cgccatgatg caggcgcata
     3301 ccaaccgcat cgggccggat gccggcgcac gaatcggcat catcgccccc gatggacgcg
     3361 atgccatgct ccagcatgcg gcgcagtttg tcgaggcggg catccccttc gtgttcgatc
     3421 cgggccaggg tctgcccatg ttcgatggcg acgagctgcg ccgtttcgtt gcgcaggccg
     3481 ggtggattgc cgtcaacgac tacgagggca agatgttgtg cgaccgcacg ggctggtctc
     3541 tggcagaact gtcgcagcag gtgaccgggc tcgtcgtcac gctgggcgcc gagggttgcg
     3601 aggtctggga gcaaggcgcc agaaccaagg tcgatcccgt gacacccgcg caggtcgtgg
     3661 accctacggg ttgcggggac gcctggcgcg gtggcctgct gtatggcctg gaaaagggct
     3721 ggcccctggt gcgctgcgcc gagctgggca atcgccttgg ggcgctgaag attgcgcagc
     3781 gcggcccaca gaactatcag ctcgacttca cgccggattg acgtaaaaaa acccggtgca
     3841 ggcaccgggt cgtggcgtgg gccggccgtg gcttacggct tgccgcccgt ggggaagggc
     3901 catgccgcct gagggctcag cgtggtctgc gctgcgggcg caggtgctgc cgcgggtgcc
     3961 ttggcggcag ccttcttcgc ggcgggggcc ttcttcacgg caggagcctt cttggcgggt
     4021 gccgcagcct tctttgcggg cgcggcggct ttcttggcca ctacggcctt cttggcgggc
     4081 gctgcggctt tcttggccgg cgcagcagcc ttcttcgccg gcgcggcagc cttcttggcc
     4141 actacggcct tcttggcggg tgctgcggct ttcttggccg gcgcagcaac cttcttcgcc
     4201 ggcgcggctg ccttcttggc cactacggcc ttcttggccg gtgctgcggc tttcttggcc
     4261 ggcgcagcag ccttcttcgc cggtgcggtt gccttcttgg ccactacggc cttcttggcc
     4321 ggtgctgcgg ctttcttggc cggcgcagca gccttcttcg ccggtgcggc tgccttcttg
     4381 gccactacgg ccttcttggc gggcgctgcg gctttcttgg ctggtgcagc ggctttcttc
     4441 gcgggagctg ccttcttggc ggcgggtttc ttcgcagttg ccatcatttt ctccttgggt
     4501 cgatttgcaa agagcactgc gcgcctgcat tgggcgcgca gcgatccatg gcaacgacac
     4561 attgctcgtc accacgaatg cggggacgca ccggcgccgc aacgctggag cgcgttgggt
     4621 gcgaggagcg tgattgaaaa atcatggcct ggacaatggg ttcaatccca ggacagggcc
     4681 ccgccggtct ggtactcgat gacgcgcgtc tcgaagaagt tgcgttcctt cttcaggtcg
     4741 atcatctcgc tcatccaggg gaaggggttt tcttcgttgg ggaacagcgc ctccagcccg
     4801 atctgtgtgg cgcggcggtt ggcgatgtag cgcaggtagc ccttgaacat ggaggcattc
     4861 atgcccagca cgccgcgcgg catggtgtcc tcggcatagc ggtattccag atccacggcc
     4921 ttgtggaaca gggcggtgat ttcttccttg aatgcggccg tccacagctg cgggttctcg
     4981 agcttgagct ggttgatcag gtcgatgccg aagttgcagt gcatggactc gtcccgcagg
     5041 atgtactggt actgctcggc ggcgccggtc atcttgttct ggcggcccag ggccaggatc
     5101 tgcgtgaaac ccacgtagaa gaacaggccc tccatcaggc aggcgaacac gatcaggctt
     5161 ttcagcagcg tctggtcgtt ctcaggagag ccggtcttga agcccggatc catgatcgcc
     5221 tcgatgaagg ggatcaggaa ctcgtccttc tcacggatgg actggatctc gttgtaggcg
     5281 ttgaagatct cgccctcgtc caggcccagc gactcgacga tgtactggta ggcgtgggtg
     5341 tggatggctt cctcaaaggc ctggcgcagc agaaactggc ggcactcggg cgccgtgatg
     5401 tggcggtagg tgccgagcac gatgttgttg gccgccagcg agtcggcggt gacgaagaag
     5461 cccaggttgc gcttgacgat gcggcgctcg tcctcggtca ggccgttcgg gtctttccag
     5521 agcgcgatgt cgcgcgtcat gttgatctcc tgcggcatcc agtggttggc gcaggtggcc
     5581 aggtatttct cccaggccca tttgtacttg aacggcacca gctgattgac atcggtctgg
     5641 ccgttgatga tgcgcttgtc cacagcggac acgcggcggt gctgtgccgc ggcggcagtg
     5701 ggcacgtgtg ccatggtgcc agtggcgcgt tgcagtgtct gggccgccaa ggcggttggc
     5761 gacggctctg cggcgcggct gcttgcgaga gcgccgtcga agaggttttg cgatgagggc
     5821 ttgacttcgt cgtcccaggt gagcatagga tttcccgtgt gtggattatg aaagcagtgc
     5881 tccaaaatga aaagcactga agcgttgctg cgccgtgttt tgttgcttca ctgcactcgg
     5941 aatgcgcctg cgcgcatccg ctggcggttg atgtcactgg caggcttcgc agcccgggtc
     6001 gtcgatggcg cagaacttca cgtcggtggc tggtattgca gcatccgcat cgagagtgcg
     6061 tgatggatga tcggcgcccg atgaaaccgc attgagctgg cgcgtgttca cggtgctcat
     6121 ctccacatgc gtggcgcttt gcgtgcgcag gtagtaggtg gtcttcaggc cgcgcagcca
     6181 ggccaactta taggtctcat cgagtttctt gcccgatgcg cccgccatgt agatgttcag
     6241 gctctgcgcc tggtctatcc atttctggcg ccgcgcggcc gcctccacca gccaggtggg
     6301 ggcgacctcg aaggctgtcg aatacaaggc tttcacgtct gcaggtacgc ggtctatggc
     6361 atgcagcgag cccttgaaat gcttcagatc catgagcatc acgtcgtccc acaggcccag
     6421 tgttttcaga tcgcgcacca ggtattggtt gatgacggtg aactcgcccg acaggttgga
     6481 cttgaccgag aggttgccga acgagggctc gatggaggcg tccacgccca cgatgttgga
     6541 gatggtggcc gtgggcgcta tggccacgca gttggaattg cgcatgccat cgcgcgcgat
     6601 cttctggcgc agggcatccc agtccagacg cgtgctggtg tccacctcaa catggccgcc
     6661 gcgttcctgc gccagcagcc cgagcgaatc cagcggcagc acgccccggt cccacagcga
     6721 gccctggaag gtggagtagc taccgcgctc gcgcgccagc tcggtcgagg cccagtaggc
     6781 gtagtaacag atggcctcca tggaagcgtc ggcaaattcc atggcggcgt cgctggcgta
     6841 gggcatgcgc agctcataca gcgcgtcctg aaagcccatc agccccaagc ccacggggcg
     6901 gtggcgcagg ttggagtcgc gcgccttgtc aacggcgtag tagttgatgt cgatgacgtt
     6961 gtccagcatg cgcatggccg tggtcacggt tctgcggagc ttgtcatgat cgatcgcgcc
     7021 atccttgagg tgctgcgcca ggttcaccga gcccaggttg cacacggccg tctcgctgtc
     7081 gctggtgttg agcgtgatct cggtgcacag gttgctggaa tgcaccacgc ccacatgctg
     7141 ctgcggcgag cggatgttgc atgggtcctt gaaggtgatc cagggatggc cggtctcgaa
     7201 cagcatggag agcatcttgc gccacagatc cacggcgggc aggcgcttga agagcttgag
     7261 ttcaccgctc tcggttttgg cctcgtaggc ggcataggcg ctctcgaagg cctggccgta
     7321 caggtcgtgc agatcgggta cgtccgacgg tgagaacagc gtccactgac cctttcccat
     7381 gacgcgtttc atgaacaggt cgggaatcca gttggcggtg ttcatgtcat gcgtgcgcct
     7441 gcggtcgtcg ccggtgttct tgcgcagttc caggaactcc tcgatgtcca ggtgccagct
     7501 ctccaggtag gtgcagaccg cgcccttgcg cttgccgccc tggttcacgg ccacggccgt
     7561 gtcgttgact accttcagga agggcaccac cccctgcgat tcgccattcg tgcccttgat
     7621 gtgcgcgccc agggcgcgca cgcgtgtcca gtcattgccc aggccgccgg caaacttgga
     7681 cagcagggcg ttttccttga tcgactcgta gatcccgccc agctcgtcgg gcaccgtggt
     7741 caggtagcag ctggacagct gtgagcgcag cgtgccgctg ttgaacagcg tgggtgtgct
     7801 gctcatgaag tcaaaagacg acagcagctc gtaaaactcg atggcgcgcg cgttgcggtc
     7861 atcttcgcgc agcgccaggc ccatggccac gcgcatgaag aagctctggg gcaactcgat
     7921 gcgtgccttg ttcacatgta ggaagtagcg gtcgtacagc gtttgcaggc ccagatagtc
     7981 gaactgctgg tcgcgctcgg cccgcaaagc ggcggccagg cgcggcaggt cgaagctcag
     8041 caggtcgggg ttgagtagtt cgttgtccac ccctttttgg acaaaggctg ggaagtgatc
     8101 catgtatgcg gcctgcatct cgccgggctg cacgccacgc cccagcacct cgcgcacgat
     8161 ggtgtgcagc agcaggcgcg ccgtggcgta gctgtagcca gggtcttttt cgatcagtgt
     8221 gcgcgcggcc aggatggagg ccttgtagac ctcctccatg ggtacgccgt catacaggtt
     8281 gcgaagagtc tcggcggcaa tcggcgcggc ctctacatcg gtgcccaggt tggcgcaggc
     8341 agactcgatc agagcctgta ggcgcagcag atccagctcc acgcgctggc cccgatcctg
     8401 cacgtacagc gccggaatct gagggtgcag ctgctccttc ttgcgcgcgc gctcctgggc
     8461 gcggcgctcg cggtacagca cataggcgcg cgcgacttcg tggtggctgc cgcgcatcag
     8521 gcccagctct acctgatcct gcacgtcctc gatatggaat gtgccgccac tggggcgcga
     8581 gcgcatcagg gcgcgcacca ccgtctgtgt cagctcatcc accacctggc gcacgctggc
     8641 cgaggccgcg ccctgcgtgc cgtgcacggc caggaaggcc ttcatcatgg cgacggcaat
     8701 cttttgcggc tcgaagtgca ctacggcgcc gttgcgtcgg atgatctggt aatggctcaa
     8761 tgtgtcggcc atgctggccg gggtggatgt gtccgcggca tcgatttgga gctgcgaaaa
     8821 gctgtcggcg tgaacggatg cggtttgcat ggcttgccct tgggagtgat gttgggggag
     8881 aagttgggaa gtgaggtcgg cccggaaaaa gcgcttgcgg gtcggcatga aaaatcagcg
     8941 gcatatcgat gccgtgcact atatatatgg tgtactttca attcaacacg ctacatatag
     9001 tgtatatccg gactttcggg tgttgtcgaa aacgctgtca gaaagcgcac gagcggtgcc
     9061 acgccaggcg gttggggttt gcgggcgtgg ggtgcagcag agatgtgcgc aggcagggct
     9121 tctggcctgc agaggcccgt caggctggct gtgccgcagg cggaaacagg atgcccgacc
     9181 ccgcaaaggc cgagcgcagg tggcgccagt caaagcccgg tccgggatcg ttcttgcgcc
     9241 cgggggcgat atgttcgtgg ccggcgacat agccgatggg gtggtgccgc accagcgccg
     9301 cgcacagccg ggtcaaggcg gcgtactgtg cctcttcgaa ggtgtggccc tccagcccct
     9361 cgagctcaat gccgatcgag tcgtcattgc agccatcgcg cccacggtag tgggatcggc
     9421 ccgcatgcca ggcgcgccgg tccgtggcca cgaactgcca gagctggccc aggcgggtga
     9481 tgaagaaatg gctcgacacc tccagcccgt ggatctgctg aaagtagggg tgggcgtccc
     9541 agtcaaggcg gttggtgaac agttcctgca cctggttgcc accatattcg ccgggcggca
     9601 ggctgatgga gtgcaccacg atcagatcca ccacggcgcc agccggccgc gccccatggt
     9661 tgggcgatgg caggcgccgc gccggcaggt accagccggc gcgccactgt ggcctattgc
     9721 ccggcgccat ggctgtcgcc agggttgggt atggcgatgt tcagccgctc gatgcggtag
     9781 cgcatctggc gctggctgac tcccaggcgg gcggccgtgg ccaccgggtc gaagctcgtt
     9841 tcgtgcaggg tttgcatgag caggccgcgc tcacgctgat ccagccaggc ctgcaggtcg
     9901 ccggctggtg gggccgtggt gggttcaggc gttggcgctt gggcgacaag cttggcggcc
     9961 ggcggcggcg gcggcggggg gggaggcggc gccgcatcgc cagcgctgct ggcttgcgtc
    10021 gggccatcga catgcagctc atgcccgtcg ctcagggcca cggcgcggtg cagcaggttt
    10081 tccagctcgc gcacattgcc cgtcagcggg tgctgggcaa tgcgctgcag cgccgcctcg
    10141 ctcagctgcg gcacggggat gccggactct tgctggatac gctccagcag cgcgtggcac
    10201 agcgccggca gatcttcgcg ccgctcgcgc agcggcggta tcaacagctc gatcacgttc
    10261 aggcggtagt acagatcctg gcggaagcgc ccggcctgca catcggccgc caggtcgcgg
    10321 tgcgtggcgc tgacgatgcg cacgtccacc gtctcttcct gtgtggagcc cagcgggcgc
    10381 acgctgcgct cctgtatggc gcgcagcagt ttggactgca tggccagcgg cagatcaccg
    10441 atctcgtcga ggaacagcgt gccgccgcgc gcggcctgaa agtagccctc gcggtcgctg
    10501 gcggcgccgg tgtaggagcc cttgcgcgcg ccgaagaact cggcctcgag caggttctcg
    10561 ggaatggcgc cgcagttcac cgccaccagc gggccgcccg cgcgctggct gctggcgtgc
    10621 agggcgcgtg ccaccagctc cttgcccgtg cccgactcgc catggatcag caccggggcc
    10681 atgctgcgcg ccaccttggc aatgcgctgc ttgacgccgc gcatggcttc ggactcgcct
    10741 atcaggcgcg ccagaaccgg gttgtcatca cctttgccgg gcgcggtgct gcggccgggt
    10801 tttgcggatg ccagcacccc tgccgccccc tggatggcag aggccacgac agagcggaac
    10861 tgtttcagat ccactggctt ggtcaggtag tcgaaggcgc ccgagcgcag cgcctctacg
    10921 gcgttctcgg ccgagccgta ggccgtcatc accacgcaac gctcgccgcg ctgctgcgca
    10981 cgcagctcgt gcagcagctc catgcccagc ccatcgggca ggcgcatgtc ggtgatcacg
    11041 gcatcgaagg tctggttttg cagttgctcg cgtgcctcgc ccacgctgcc ggcggtctgc
    11101 acgcgatagc cctcgcgcag caaggtcagc tcgtagagcg tgcgcaggtc gggctcatcg
    11161 tctacgacaa gaatggtggc ggcggtggcg ttcatggatg tcggtcgctt agaccacgat
    11221 gatgtcgaac agtgagtcgg tttcaaaggg gcgggtggcg ctgcggaaac tgacggtaaa
    11281 ggcattgccc atcaccgtgc cgcggtcgag catgcgtgtg ctgcgctggt agctgatggc
    11341 cgcgccatgg cgctggcaca gctggcggca aatgtacagg cccaggccgc tggagcggct
    11401 ctcggacgag aaaaacggct cgaacaaatg ccgctccacc gatttgtcca gtggcgcacc
    11461 atcgctccag acctgtatgc cgatctgccc gctggctgtg gcgcgtgtgc tcagttgcag
    11521 tgagtcggcg tacggaccct tgtagcgctg ggcgttgtcc agcaggttca ccagcacgcg
    11581 gcgcaggtgc tcggggtcga actccaccgg tacctcgccc gcccccagca acagctgccc
    11641 ctggcgaccc gcggggtcat gggcctgcca gtcatgccag gtctgtgcca ccgtggcatt
    11701 cagcgccagc gtggaggacg gggtctggcc tatctggtgc tgtacccggg cgatgtccag
    11761 cacatcttcg gtgatgcgca ccaggcgatc cgcgttctgc ccgatcatgt ggctcaggcg
    11821 cttctgcccg ggttcttgca gatcctcggc cagcagctcg ttggcctgca cgatggccgc
    11881 cagcgggttg cgtatctcat gcgccaccgc ggccgacatg cggcccatcg aggccagctt
    11941 ctcggtgcgc aggcgcgcct ccatctcgcg caggtcgtgc agaaacatca cgcacagggg
    12001 ctcctcgtcc actgccccca aggcgccggg tgcgtgcgtc agccaggtgc gtatgcgcag
    12061 gcccatgggg ctatgcccct cgtgcaggat gttcacgtcg gcatgctggg gctgtgcctg
    12121 ctcgaacgat gcctgcgcca gctccatcag cggactccag gcctgcgtat gggtcagcat
    12181 gaacggggtg ctggttctgg cgtccgggcc cagcagcagc agggcggccg ggttggccag
    12241 gcgcacctgg ccctggcggt tgagcaccag cacgccgtcg gccacatgct gcatcaccag
    12301 tgcgctcacg gccttttgcg tgcgcgcatt catctggctg cgccgcgcca ccagttgctc
    12361 gcggctcagg cggacggcca gctgatgcac cagataggca atgaagaaat agcccgtgcc
    12421 agtcagcgcc gtttgcaggt agcgctgcgc ggcgtcgccc ccgccctggt cgcccagcca
    12481 ccaggcccag gccagcagca gcagggtggc ccccgccgtg gtgcccaggg ccagcgtcag
    12541 cgtgcccagc accgcggcca tgaggatggg caggccaaac agcggcgtga agttcatgct
    12601 gcccgactgg tgcagcatct gcagcaaggc aatgaggacg agatccacgc caatggaggg
    12661 cagccagtgc aggcccggtc gcggatccgg tggcatgcgt gcgccaaata ccagcagcaa
    12721 tgacgtggcc agcaagtagc ccagtgccac gcccagcggc gccagcacac ccgtctggtt
    12781 cagcgccatg gccagcagct gcagcaacag cagcgccagc gccaccatca ggcgggccgt
    12841 caggaacgcc ttccacaggc gctggaagga ggggtggctg gcctggagtg cgggcatggg
    12901 ggatcgcctc tcaccctctt agggggggcc cagatcctgg tgcgcgcggc tgcaatagct
    12961 gtgcccgccc tggtgcaagg cctcaccctt gggcaggtgc acgccacaat gcgcgcagca
    13021 caccatgtcc tggggctgcg cagggttctg gcgcggtgcg ctcccccggt tgggcggtac
    13081 ctggcgctgg ctgcgccaca gggcgtagac cacggcaatc accgccagca cgatgaggta
    13141 cttcatgtcg tgcgccccag caccacttca agcacgaagc gcgagcccac ataggccagc
    13201 agcagcagca tggcgcccgc atacagcacg cgcacggcgt tgcggccgcg ccagccaaag
    13261 cgtgcgcgcc ccagcagcag cacggcaaag gtgacccagg ccagcaccga gaacaccgcc
    13321 ttgtgatccc acagccaggc gcggccgtag agctgctcgc tgaacagcca gcccgccacc
    13381 agcgtggccg acagcagcgc aaagccccag cgcacgaagc gaaaggtcag gcgctccagc
    13441 gtgagcagcg gcaggctgct gtgcagttgg gtggcctggc gcatctgccg ctccgcgcgg
    13501 ctcatcagcc aggcatgggc cacggcggcg gcaaacaggc catagcaggc aatgcccagg
    13561 gtcaggtgca gcggcatcca gggtgatgcc gtggcctgca gtggccggcc cggaaacagc
    13621 gccgccagca gcacggccac gccgcccagc gcgcccagcg cccagcgcgt gcgcatctgc
    13681 ggaaacagct cgcgctccag cgcgtagatg gtcagcacca gccaggccgt catcgacagc
    13741 gcggcggcaa atccgaagtg gggcgcaccg cccagctggc tccagccaat ggcgccggca
    13801 tgcaacgccc aggccggcgc catcgccagg tggctgcggc tggcctggct gcgcgaggcc
    13861 acggccgcgc caatcgcata ggcggcggcc gtcaccaggg ccagcagcca tgttgacggg
    13921 gaagcactgg gtaaaatcat gggattgaag tttagcccct tgcccctggt tcccagggcc
    13981 gggccaccgg cagccggcaa gcccagcgcc gataccactg gagcacttgg cgcaatggcc
    14041 tccgcactca ccgacaaact cacccgtctc gtcaaggaga tgcgcggcca ggcccgcatc
    14101 accgaatcca acgtccagga catgctgcgc gaggtgcgca tggcgctgct ggaggccgac
    14161 gtggccctgc ccgtggtgcg cgacttcatc gcccgcgtca aggacaaggc cctggg
//
LOCUS       DAOMTQ010000075        14191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_699_length_14191_cov_24.325623, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000075 DAOMTQ010000000
VERSION     DAOMTQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14191
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_14191_cov_24.325623"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK518_08820"
     CDS             <1..794
                     /locus_tag="PK518_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM49058.1"
                     /translation="AEQHAVTFAAGMACEGVKPVVAIYSTFLQRAYDQLIHDVALQNL
                     PVVFALDRAGLVGADGATHAGAYDIAFIRCIPNMSLACPADERECRQLLTTAYEQDHP
                     VAVRYPRGSGAGVAPLATLDGLPFGKAEVRRQGRRIAILAFGTLLYPALQAAEKLDAT
                     VVNMRWAKPLDTELLLQVAAEHEALVTVEEGCIMGGAGSAVAEALHAAGVQRPLLQLG
                     LPDTFIEHGDPAKLLALQGLDAQGIEHAIAERFLAPAMLAPRAVA"
     gene            963..2045
                     /gene="dctP"
                     /locus_tag="PK518_08825"
     CDS             963..2045
                     /gene="dctP"
                     /locus_tag="PK518_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRM49059.1"
                     /translation="MDRRSLIKNAGLAGVLAAGMAPAVHAQAAVRWRLATSFPKSLET
                     LHGCAVKFSETVKALSGGKFEVSVHAAGELVPAFGIVDALADDSIEMGETAAYYYTGK
                     DPAFAFGCAVPFGFTPQQIQAWKLYGNGHKLMDELYGKYNFFGLSALNTGTQMGGWYR
                     KEMKSVADFKGLKMRLGGGVFGESMAKLGVVAQNMPGGDVYQALEKGTLDAAEFVGPY
                     DDEKLGWNKVAKYYYYGAWWEGGADLEFFINKKAFAKLSPENQAIVHAAASVAYTDGT
                     SKYLMLNPIALKKLVAGGTQLKRFDKAIVQAGFKAAQEVYAEHSAKDAFFKKVFQDMR
                     TFQRDQILWNRISELAYTQDIAGLKL"
     gene            complement(2158..3972)
                     /locus_tag="PK518_08830"
     CDS             complement(2158..3972)
                     /locus_tag="PK518_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRM49060.1"
                     /translation="MEFIAANFAPVMFAGLILFLLLGFPVAFSLGAAGLFFGLLGIEL
                     GVFTTAIFSWLPQRLIGIMANETLLAVPFFTLMGLILERSGMAEDLLETVGQVFGPLR
                     GGLALAVVLVGAMLAATTGVVAASVISMGLISLPIMLRYGYDRSLSAGVIAASGTLAQ
                     IIPPSLVLIVLADQMGKSVGDMYKGAFIPGFALMGLYILWILALAIFKPQSVPALPPE
                     ARTFREPSGSSGYTSLTVLMLISTLAAVFLAEHMEAVHSWWQGELVEFVPTDEKIVVA
                     MCGGSFLAWLIAALNKVTRLGLLSKLAERVTFVLIPPLLLIFLVLGTIFLGVATPTEG
                     GAMGAVGALIMATLRRRLDLPLLKQALNTTTKLSSFVMFILVGATIFSMVFQAADGPV
                     WVEHLLAKLPGGQLGFLIFVNVMIFVLAFFLDFFELSFIVVPVLAPIADKMGIDLVWF
                     GVLLAVNMQTSFMHPPFGFALFYLRSVAPDKPYVDSHTGKTMEPVTTMQIYKGAIPFL
                     VLQLSMVAILIAFPGLVTSNLDKQVNVDMKAVGEQMLEQLNEEEAEPELDLGIGSPDT
                     AAPADTGSALPEPGATEGAPAAPEDDPVKALDQSVGKP"
     gene            complement(3998..4597)
                     /locus_tag="PK518_08835"
     CDS             complement(3998..4597)
                     /locus_tag="PK518_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRM49061.1"
                     /translation="MQALLALSRVIDRLNALIGKYAIWLIFAATVISALNAIVRKAFN
                     YSSNGFLEVQWYLFAWSFLIAAGYTLLNREHVRIDVLNAHFSKRAQIWIEILGVILFL
                     TPVCVLVLWLSIPIVGERYASGEMSPNPGGLIRWPVWMALPVSFSLLLLQGWSELIKC
                     LAFLRGQGPDPTVKIDAKSTEQELVDALRQQQTASGQAH"
     gene            complement(4800..5765)
                     /locus_tag="PK518_08840"
     CDS             complement(4800..5765)
                     /locus_tag="PK518_08840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRM49062.1"
                     /translation="MSSNLHPMLNVAIKAARAAGALINRAALDVEAVRISQKQINDFV
                     TEVDHAAERAIIETLLTAYPGHGILAEESGSEHGAKHSDYVWIIDPLDGTTNFIHGFP
                     VYCVSIAMAFKGKVEHAVVYDPSRNDLFTATRGRGAFLNERRIRVSKRTQLKDCLVST
                     GFPFRPGDNFKQYMAMMAELMPRTAGLRRPGAAALDLAYVAAGYCDGFFESGLSIWDV
                     AAGSLLVTEAGGLVGNFTGEADFMEQRECLAGSPRIYGQLVGLLGKYSKFAPAGDKAA
                     VRQALANGADGAADDQSVDMGADADVTAHPDSAAEDQQTRPSAAP"
     gene            5917..6699
                     /locus_tag="PK518_08845"
     CDS             5917..6699
                     /locus_tag="PK518_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRM49063.1"
                     /translation="MRTRFILIHTSHAGNVGAVARAMKTMGFDDLVLVAPRWANVLRK
                     DETIQRASGALDVLAQARIVATLDEALDGMSHLCATAMTPRDFGPPTRTPREHFDLLL
                     KGELSALDGRAQEANFAENYSSESGVAFLFGSERFGMANEDVYRCHVALSIPTNPQFG
                     SLNLGAAVQVIAYEWRQALGAYNAPESAPQGDRADAAQVAGMLAHWEQALAAIGFLDP
                     AAPKKLMPRLNQMFNRAQLTQEEIHILRGVAKSMLQAARPNG"
     gene            6736..7527
                     /gene="cysE"
                     /locus_tag="PK518_08850"
     CDS             6736..7527
                     /gene="cysE"
                     /locus_tag="PK518_08850"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRM49064.1"
                     /translation="MLARLQSDIQCILDRDPAARSRFEVVTCYPGLHAIWLHLPAHWC
                     WTHGFKWLGRFISHLARWFTGIEIHPGAVIGERVFIDHGMGVVIGETAVVGDGCTIYH
                     GVTLGGTSLYKGAKRHPTLGRDVVVAAGAKVLGGFEVGEGAKIGSNAVVIKPVPAGAT
                     AVGIPARIIPSKEGQSADVTEPHGQEEAKFSAYGVTAQQDDPLTQAMRGLIDSAAGQE
                     HQIALLWQAIEKLSQTTPQGQDCVPGDAALSGHFPAAKLNDIIGK"
     gene            7546..8208
                     /locus_tag="PK518_08855"
     CDS             7546..8208
                     /locus_tag="PK518_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRM49065.1"
                     /translation="MDVTQALRQRRSVRAFLPDAPPAALVQTLLQEAAQAASGGNLQP
                     WRVVALTGEPLRQVLAAVATAPAEDAPTNQSYPPSLWEPYRTRRFANGEDLYRTLGIP
                     REDKAARLEQLAKNAEFFGAPVGIFVCVDERMGLPQWADLGIYLQSLMLLAVHHGLAT
                     CAQGFWRRYAGTLQRQLALPEPYHVACGVALGYEDEAAPINALRSTRAPWADWGELRG
                     FD"
     gene            complement(8327..9424)
                     /locus_tag="PK518_08860"
     CDS             complement(8327..9424)
                     /locus_tag="PK518_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRM49066.1"
                     /translation="MTAMMKAAVFVDKGRIELADKPIPDVGPNDALVRITTTTICGTD
                     VHILKGEYPVAKGLTVGHEPVGVIEKLGSAVQGYQEGQRVIAGAICPNFNSYAAQDGA
                     PSQDGSYLVPRGQCGCHGYKATAGWRFGNLIDGTQAEYVLVPDAQANLAPIPDGLTDE
                     QVLMCPDIMSTGFKGAENANIRIGDTVVVLAQGPIGLCATAGARLLGATTIIAVDGND
                     QRLAVAKKMGADVTLNFRNVDVVAEVMKLTGGKGADSSIEALGTQATFAQAMQVLRPG
                     GTLSSLGVYSEDLSIPLAQFAAGLGDHTIRTALCPGGKERMRRLMNVIAANRLDLGVL
                     VTHQRRLDEIVEAYDLFAHQRDGVLKIAIKP"
     gene            complement(9507..10115)
                     /gene="mog"
                     /locus_tag="PK518_08865"
     CDS             complement(9507..10115)
                     /gene="mog"
                     /locus_tag="PK518_08865"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HRM49067.1"
                     /translation="MPHTAHEYEPVKIGIVSISDRASSGDYEDKGLPALHDWLKRALH
                     NPLTFEPRLIPDEKDRISATLVELVDAGCSLVLTTGGTGPALRDVTPEATLAVADKEM
                     PGFGEQMRQISLKFVPTAILSRQVAVIRGKSLIINLPGQPKAIAETLEGLRDEAGAQL
                     VPGIFAAVPYCIDLIGGPYLETRPELCKAFRPKSAMRPTPAR"
     gene            complement(10096..10779)
                     /gene="yjgA"
                     /locus_tag="PK518_08870"
     CDS             complement(10096..10779)
                     /gene="yjgA"
                     /locus_tag="PK518_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HRM49068.1"
                     /translation="MSRKPKKGYFVKGHFVAEGSEMDLQFKAELKGTTGATKTELKRE
                     MEALQVLGADLLSLRADLFARLELPEKLQDALAEAKRITNFEGKRRQMQFVGKLMRKL
                     GDDTVAAVQAALDEQHGGSAAEKLALHQAEHWRDRLIAGDEALPLWLQQFPDTDTQQI
                     RALIRQARKDAPPEDKAAVSQGLAPRKGRAYRDLFQLVRAQMLAGQAHNEALPDTHDE
                     EDGHAPYRP"
     gene            10858..12279
                     /gene="pmbA"
                     /locus_tag="PK518_08875"
     CDS             10858..12279
                     /gene="pmbA"
                     /locus_tag="PK518_08875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HRM49069.1"
                     /translation="MNTPSSRAKSAAPAQHPTQPESGFSYTREFFAGLVDHALAHAKK
                     LGASDAGADASEGCGLSVNVRKGELETVERNRDKSLGVTVYLGHRRGNASTSDFSSAA
                     VERTVQAAYDIARFTAEDPVAGLPDEADIAPQDSHRDLQLFHPWAVTSEEAAQLALQC
                     EAAALKTHRRITNSEGAGVSAQQSHFFSAHTRGFRGGYASSRHSISVAPIASLPGRNG
                     EMQRDAWYSSMRDARELAAPEAVGRYAAQRALARLGSRKIPTTECPVLFESPLAAGLL
                     GSFVQAISGGALYRKTSFLVDSLDKQVFAKHINVSEDPFILGGKGSSPFDDEGVRVAP
                     RQVVKGGRVQGYFLSTYSARKLGMKTTGNAGGSHNLVLTSRQTKEHDDLDAMLQKLGT
                     GLFVVELMGQGVNYVTGDYSRGASGFWVENGRIAYPVHEITIAGNLKTMLRGIEAVGA
                     DAYNYGAKTVGSILINRMKVAGS"
     gene            complement(12602..13246)
                     /locus_tag="PK518_08880"
     CDS             complement(12602..13246)
                     /locus_tag="PK518_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic enzyme"
                     /protein_id="HRM49070.1"
                     /translation="MTVRITEHDVATLAPSARSSYREAFINAQPVLDLFGISDSPLRM
                     AHFMAQMLHECGGLTIQFENLNYSAARLPKVWPSRFQPTGPLNPAAFAHNPQKLANEV
                     YGKRMGNTAPDDGFTYRGRGLLQLTGKDSYQRATQILRGSFADAPDFVQAPDEVISAG
                     WCVTVAAAIWQQKGCNEKADADSITQVTKAINGGLIGLGDRKEWLRRTRAVWAG"
     gene            complement(13584..>14191)
                     /locus_tag="PK518_08885"
     CDS             complement(13584..>14191)
                     /locus_tag="PK518_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49071.1"
                     /translation="LTIRRSRPPTASAELRVRHQQTSKETSIDSIFRFGAALLVALLV
                     GFASGFALLVFAPCSWFGSSFEGACGYGAFGFAVVLGLLVFTIAFALLWLGFRHKLSP
                     PSPVPNTEPPSGLYGSWLALLVLQYVGPLLLASVPGAGMFSIVTSILLLAAFIVTSAL
                     LARYRARHPVAAFFALVPLAGPLIVGVLLLKPRSPVPSGGA"
BASE COUNT         2548 a         4600 c         4694 g         2349 t
ORIGIN      
        1 ttgccgagca gcatgccgtg acctttgccg ccggcatggc ctgcgagggc gtgaagcccg
       61 tggtcgccat ctactccacc tttttgcagc gtgcctatga ccagctgatc cacgacgtgg
      121 cactgcagaa cctgccggtc gtctttgccc tggaccgcgc cggtctggtc ggcgccgacg
      181 gcgccacgca cgccggtgcc tatgacatcg ccttcatccg ttgcatcccc aacatgagcc
      241 tggcctgccc ggccgacgag cgcgaatgcc gccagctgct caccacagcc tacgaacagg
      301 atcatcccgt ggccgtgcgc tacccgcgtg gcagcggcgc gggcgtggca ccgctggcca
      361 cgctggacgg cctgcccttc ggcaaggcag aggttcgccg ccagggccgg cgcatcgcca
      421 tcctggcttt tggcacgctg ctctacccgg cgctgcaggc ggccgagaag ctggacgcca
      481 ccgtggtcaa catgcgctgg gccaagccgc tcgacaccga actgctgctg caggtggcgg
      541 ccgagcacga ggcgctggtg acggtggagg agggctgcat catgggcggc gccggcagcg
      601 ccgtggccga ggccctgcac gccgccggcg tgcagcgccc gctgctgcag ctgggcctgc
      661 ccgacacctt catcgagcat ggcgacccgg cgaagctgct ggcgctgcag gggctggatg
      721 cccaggggat cgagcacgcc attgccgagc gttttcttgc cccggcgatg cttgcaccgc
      781 gcgccgtagc ctgaacgggc gcaggccttt gtccggcgat tgtttgcaat agggggcaat
      841 cgccctggcg agcctttgcg ggtagcaata attaattaca ctcgaacggc tctcatggca
      901 gccagcgatg ctgccgcctg caagagttgt gtttggtgat caatcaattg gagatattgc
      961 tcatggatcg tcgttccctc atcaagaatg ctggcctcgc cggcgtgctg gccgctggta
     1021 tggcacccgc ggtgcatgcg caagccgcag tgcgctggcg cctggcgacc agcttcccta
     1081 agtcgctgga gaccctgcac ggctgcgccg tcaagttctc cgagaccgtc aaggcgctgt
     1141 cgggtggcaa attcgaggtc tcggtgcacg ccgcgggcga gctggtgccg gcgttcggca
     1201 tcgtcgatgc gctggccgac gattcgatcg agatgggcga gaccgccgcc tactactaca
     1261 ccggcaagga cccggccttt gccttcggtt gcgccgtgcc gtttggtttt acgccgcagc
     1321 agatccaggc ctggaagctg tatggcaacg gccacaagct gatggacgag ttgtatggta
     1381 agtacaactt ctttggcctg agcgcgctca acaccggcac gcagatgggc ggctggtacc
     1441 gcaaggagat gaagtcggtt gcagacttca agggcctgaa gatgcgcctg ggcggcggcg
     1501 tgttcggcga atcgatggcc aagctcggcg tggtcgcgca gaacatgccc ggcggcgatg
     1561 tctaccaggc gctggagaag ggcacgctgg acgcggccga gttcgtcggc ccctacgacg
     1621 acgaaaaact cggttggaac aaggttgcca agtactacta ctacggcgcc tggtgggaag
     1681 gcggcgcgga cctggagttc ttcatcaaca agaaggcctt cgccaagctc tcgcccgaga
     1741 accaggccat cgtgcatgcg gccgcatccg tggcctatac cgacggcacg tccaaatacc
     1801 tgatgctcaa cccgatcgcg ctcaagaagc tggttgctgg cggcacgcag ttgaagcgct
     1861 ttgacaaagc catcgtccag gcaggcttca aggccgcgca agaggtctat gccgagcaca
     1921 gcgccaagga tgccttcttc aagaaggtgt tccaggacat gcgcaccttc cagcgcgacc
     1981 agattctgtg gaaccgtatt tcggaactgg cctacaccca ggacatcgcc ggcctgaagc
     2041 tgtaaaccga ctctggtggg gggcgctaac gcctcccgca gccagaaaaa agccccgtgc
     2101 catgaatgtg gccggggcat ttttcatggt cgcgcccggg agggcgcggc tgcaaagtta
     2161 gggcttgccg acggactgat ccagggcctt gaccgggtcg tcttccggcg ctgcgggcgc
     2221 gccttcggtg gcgccgggct ctggcagtgc gctgccggtg tcggcgggcg ctgcggtgtc
     2281 ggggctgccg atcccaaggt ctagctcggg ctctgcttcc tcttcgttga gttgctccag
     2341 catctgctcg cccacggcct tcatatctac gttgacttgc ttgtccaggt tgctcgtcac
     2401 caggccgggg aaggcgatca ggatggcgac catggacagc tgcagcacca ggaagggaat
     2461 cgcgcccttg tagatctgca tcgtcgtcac cggctccatg gtcttgccgg tgtggctgtc
     2521 gacgtagggc ttgtccggcg cgaccgagcg caggtagaac agcgcgaagc caaacggcgg
     2581 gtgcatgaac gaggtctgca tgtttaccgc cagcagcacg ccaaaccaga ccaggtcgat
     2641 gcccatcttg tcggcgatgg gcgcaagcac cggcacgacg atgaaggaca gctcgaagaa
     2701 gtccaggaag aacgccagca cgaagatcat cacgttgacg aagatcagaa agcccagctg
     2761 gccgccgggc agcttggcca gcaggtgctc gacccacacc gggccgtcag cggcctggaa
     2821 caccatgctg aagatggtgg cgccgaccag gatgaacatc acgaaggagg acagcttggt
     2881 cgtggtgttg agcgcctgct tgagcagcgg caggtccagg cgccggcgca gcgtggccat
     2941 gatcagcgcg ccgaccgcgc ccatggcgcc gccctcggtg ggtgtggcca cgcccaggaa
     3001 gatggtgccc agcaccagga agatcagcag cagcggcggg atcagcacga aggtcacgcg
     3061 ctcggccagc ttggacagca ggcccaggcg cgtgaccttg ttgagcgcgg cgatcagcca
     3121 ggcgaggaag ctgccgccgc acatggcgac gacgatcttc tcgtcggtgg ggacgaactc
     3181 gaccagttcg ccctgccacc agctatgcac ggcctccatg tgctcggcca ggaagacggc
     3241 ggcgagcgtg gagatcagca tcagcacggt cagcgaggta tagcccgagc tgccgctggg
     3301 ttcgcgaaac gtccgcgcct cgggcggcag cgccggcacc gactggggtt tgaagatggc
     3361 gagtgccagg atccacagga tgtacagccc catcagcgcg aagccgggga tgaaggcgcc
     3421 cttgtacatg tcgcccacgc tcttgcccat ctggtcagcg agcacgatca gcaccaggga
     3481 tggcgggatg atttgcgcca gcgtgcccga agcagcaatc acgccggcgc tgaggctgcg
     3541 gtcgtaacca tagcgaagca tgatgggcag agagataagg cccattgaga tcactgaggc
     3601 cgcgacaacc ccggtagttg ccgccagcat cgcgccgacc agcaccacgg ccaacgccag
     3661 gccgccgcgc agtgggccga acacctggcc gacggtctcc agcagatctt cggccatgcc
     3721 actgcgctcc agaatcaggc ccatgagcgt gaagaagggc acggccagga gtgtctcgtt
     3781 ggccatgatg ccgatgaggc gctggggcag ccagctgaag atggcagtgg taaagacgcc
     3841 cagctctatc cccagcagac caaaaaatag gccggcggcg cccagactga aggcgaccgg
     3901 aaagccgagc agcagaaaaa gaatcaaccc ggcgaacatc accggagcga aattggcagc
     3961 aatgaattcc atgatgagtt aggccttgga cagtgggtca gtgggcctgg ccgctggcgg
     4021 tttgctgctg acgcagcgca tcgacgagct cttgttcggt gctttttgcg tcgattttca
     4081 ctgtcggatc gggcccttgg cctcgcagga aagcgaggca tttgattaat tcagaccaac
     4141 cctgaagcaa cagaagggag aagcttacgg gtagcgccat ccacactggc cagcgaatca
     4201 ggccaccggg gttgggtgac atctcgcccg aagcgtagcg ctcgccgacg atggggattg
     4261 acaaccacag caccagtacg cagacgggtg taaggaagag gatcactccc agaatctcga
     4321 tccagatctg cgcgcgcttg gagaagtggg cgttgagcac gtcgatgcgg acatgctcac
     4381 ggttgagaag ggtgtagccg gcagcgatca gaaacgacca cgcaaacaga taccactgca
     4441 cctccaaaaa gccattggag ctgtagttga aggccttgcg aacgatggcg ttgagggcgc
     4501 tgatgaccgt ggcagcaaat atcagccaga tggcgtattt gccgatcaag gcgttgagtc
     4561 tgtctatgac ccgcgatagg gcgagtaaag cctgcatggt gtctccagga tggggatgtg
     4621 tgtagccacg tagcctgcag ccgtagtgat gttcctgcac gatcgttaga acatacaggc
     4681 ctgcgcaccg ggtgctacag gtttttacaa ggatcgcatt ctacggagcc atgacccagg
     4741 tcacgcatgg cctggacggt aagttgcatg ctgagaccga ttggcgtgcg cgttggtgct
     4801 caaggcgcag cgctcgggcg cgtctgctgg tcctcggcgg cgctgtcggg gtgcgcggtg
     4861 acgtctgcgt ccgcgcccat gtccacagac tggtcatcgg ctgctccgtc ggcaccattg
     4921 gccagcgcct ggcgcactgc cgccttgtcg cccgctgggg cgaacttgct gtacttgccc
     4981 aagaggccca ccagctggcc gtagatgcgc ggcgagccgg ccaggcattc gcgctgttcc
     5041 atgaagtcgg cctcgccggt gaagttgccc accaggccgc cggcctcggt caccagcagg
     5101 ctgccggcgg ccacgtccca gatcgacagg ccggactcga agaatccgtc gcagtaaccg
     5161 gcggccacat aggccagatc cagcgcggcg gcgccggggc ggcgcaggcc ggcggtgcgc
     5221 ggcatcagct cggccatcat ggccatgtat tgcttgaagt tgtcgcccgg gcggaagggg
     5281 aagccggtgg agaccaggca gtccttgagc tgcgtgcgct tggagacgcg gatgcgccgc
     5341 tcgttcagaa aggcgccgcg cccgcgcgtg gcggtgaaca ggtcgttgcg cgaggggtcg
     5401 tagaccacgg cgtgctccac cttgcccttg aaggccatgg cgatgctcac gcagtacacc
     5461 ggaaagccgt ggatgaagtt ggtcgtgccg tccagcgggt cgatgatcca gacgtagtcc
     5521 gaatgcttgg cgccatgctc gctgcccgat tcctcggcca ggatgccgtg gccggggtag
     5581 gcggtgagca gggtttcgat gatggcgcgc tcggccgcgt ggtccacctc ggtcacaaag
     5641 tcgttgatct gcttttgcga aatgcgcacc gcctcgacat cgagggccgc gcggttgatg
     5701 agggcgccgg cggcgcgggc ggccttgatg gccacgttga gcatggggtg cagattggac
     5761 gacataggtt gtgactaagg agcggagggc ggctcgtggc cggggcatgc ggccggcgat
     5821 gcgggcgcaa agcgcgcatt ttagccgcgc ccccaaatcc tgcgcagtgc gggtggctgc
     5881 ggcgacaatc accggctctt tcatccccac tgcgccatga gaacccgttt catcctcatc
     5941 cacaccagcc atgccggcaa cgtgggcgcg gtcgcgcgcg ccatgaagac catgggcttt
     6001 gacgacctgg tgctggtggc gccgcgctgg gcgaatgtgc tgcgcaagga tgaaaccata
     6061 cagcgcgcca gcggcgccct ggatgtgctg gcccaggcgc gcatcgtggc cacgctggac
     6121 gaggcgctcg acggcatgag ccacctgtgc gccacggcca tgacgccgcg cgacttcggc
     6181 ccgcccacgc gcacgccgcg cgagcatttt gatttgctat tgaaaggtga gctgtctgcg
     6241 cttgatggta gggcgcaaga ggctaatttt gctgaaaact attcgtccga atccggcgtc
     6301 gccttcctgt ttggctccga gcgtttcggc atggccaacg aggatgtcta tcgctgccat
     6361 gtggcgctca gcattcccac caatccgcag tttggctcgc tgaacctggg cgccgcggtg
     6421 caggtcatcg cctacgaatg gcgccaggcc ctgggggctt acaacgcgcc cgagtccgcg
     6481 ccgcagggcg accgggccga cgccgcgcag gtggccggca tgctggcgca ctgggagcag
     6541 gcgctggcgg cgatcggctt tctggatccg gcggcgccca agaagctcat gccgcggctg
     6601 aaccagatgt tcaaccgcgc acaactcacg caggaggaaa tccatatcct gcgcggtgtc
     6661 gccaaatcca tgctgcaggc cgcacggcca aacggctaga cttcaaagcc ttttttgtca
     6721 ccgccacagc ccttcatgct tgcccgcctg caatcggata tccagtgcat ccttgaccgc
     6781 gacccggcgg cgcgctcgcg cttcgaggtc gtcacctgct acccgggcct gcacgccatc
     6841 tggctgcacc tgcccgcgca ctggtgctgg acgcatggtt tcaagtggct ggggcgcttc
     6901 atctcgcacc tggcgcgctg gttcacgggt atagagatcc accccggcgc cgtgatcggc
     6961 gagcgcgtct tcatcgacca cggcatgggc gtggtgatcg gcgagacggc cgtggtgggc
     7021 gacggctgca ccatctacca cggcgtgacg ctgggcggca cctcgctgta caagggcgcc
     7081 aagcgccacc ccacgctggg gcgcgatgtg gtggtggccg cgggcgccaa ggtgctgggc
     7141 ggcttcgagg tgggcgaggg ggcaaaaata ggcagcaatg ccgtggtcat caagcctgtg
     7201 cccgccggcg ccacggctgt gggcataccg gcgcgcatca tcccgtccaa ggaaggccag
     7261 agcgccgacg tgaccgagcc gcacgggcag gaggaagcca agttcagcgc ctatggcgtg
     7321 accgcgcagc aggacgaccc cctcacccag gcgatgcgcg gcctgatcga cagcgccgcc
     7381 ggtcaggagc accagatcgc gctgctctgg caggccatag aaaagctctc gcagaccacg
     7441 ccccagggcc aggactgcgt gcccggcgac gccgcgctca gcgggcattt cccggccgcc
     7501 aagcttaacg acatcatcgg caagtagccg ccgacaggag agaccatgga cgtaacccag
     7561 gccctgcgcc agcgccgctc ggtgcgcgcc tttctgcccg acgcaccgcc cgccgccctg
     7621 gtgcagaccc tgctgcaaga ggctgcccag gccgcgtccg gcggcaatct gcagccctgg
     7681 cgcgttgtgg cgctgacggg tgaacccctg cggcaggtgc tggccgccgt ggccacggcg
     7741 ccggccgagg acgcgccgac caaccagtcc tacccgccca gcctgtggga gccctaccgc
     7801 acgcgccgtt ttgccaatgg cgaggacctg taccgcaccc tgggcatacc gcgcgaggac
     7861 aaggccgcac gcctggagca actggccaag aacgccgagt tctttggcgc gcccgtgggc
     7921 atcttcgtct gcgtggacga gcgcatgggc ctgccgcaat gggccgacct gggcatctat
     7981 ctgcagtccc tgatgctgct ggccgtgcac catggcctgg cgacctgcgc ccagggcttc
     8041 tggcgccgct acgccggcac gctgcagcgc cagctggccc tgcccgagcc ctatcacgtg
     8101 gcctgcggcg tggccctggg ctacgaggac gaggccgcgc ccatcaacgc gctgcgctcc
     8161 acgcgcgcgc cctgggccga ctggggcgag ctgcgcggct ttgactgaga tatgttgata
     8221 gcgcctcgcg cttgtttggc gagggtttta actccctctc ccgcctgcgg gagaggggga
     8281 aatgacggcc agccctggac ggcagcctgc agcgtccgcc aagccatcac ggcttgatgg
     8341 cgatcttcag caccccgtcg cgctggtggg caaacaggtc ataggcctcc acgatctcgt
     8401 ccagtcggcg ctggtgcgtc accagcacgc ccagatccag gcgattcgcg gcaatcacgt
     8461 tcatcaggcg gcgcatgcgc tccttgccgc cggggcacag cgcggtgcgt atggtgtgat
     8521 cgcccaggcc ggcggcaaac tgcgccagcg gaatggacaa atcctccgaa tacaccccca
     8581 ggctcgacag cgtgcccccg ggccgcagca cctgcatggc ctgggcaaac gtggcctggg
     8641 tgcccagggc ctcgatggag ctgtcggcac ccttgccgcc ggtgagcttc atcacctcgg
     8701 ccaccacgtc cacgttgcgg aagttcagcg tcacgtcggc gcccatcttt ttggcgactg
     8761 ccaggcgctg gtcattgccg tccacggcga tgatggtcgt cgcccccagc aggcgcgcgc
     8821 cggccgtggc gcacaggcct atcgggccct gcgccagcac caccacggtg tcgccaatgc
     8881 ggatgttggc gttctcggcg cccttgaagc cggtggacat gatgtccggg cacatcagca
     8941 cctgctcgtc cgtcaggcca tcgggaatgg gcgccaggtt cgcctgggcg tccggcacca
     9001 gcacatattc ggcctgcgtg ccgtcgatca ggttgccaaa gcgccagccc gccgtggcct
     9061 tgtagccgtg gcagccacat tgaccgcgcg gcacaagata gctgccgtcc tggctgggcg
     9121 cgccgtcctg cgcggcgtag ctgttgaagt tggggcagat ggcgccggca atcacgcgct
     9181 ggccctcctg gtagccctgc acggcgctgc ccagtttctc gatcacgccc accggctcat
     9241 ggcccacggt caggcccttg gcgaccgggt actcgccctt gaggatgtgc acatccgtgc
     9301 cgcagatggt ggtggtggtg atgcgcacca gcgcgtcgtt ggggcccacg tcgggtatgg
     9361 gcttgtcggc cagctcgatg cggcccttgt ccacaaaaac ggcagctttc atcatggcgg
     9421 tcatggtggc actcctttcg tgtgaaagga tcaccatagg ccatggaggt ggccgccggc
     9481 ttacatcgca gcggcctgct cgggtatcac cgcgcgggcg taggccgcat ggccgacttg
     9541 gggcgaaacg ccttgcagag ctcgggccgg gtctccaggt agggtccgcc tatcaggtcg
     9601 atgcagtagg gcacggcggc aaaaatgccc ggcaccagtt gcgcgccggc ctcgtcacgc
     9661 aagccctcca gcgtctcggc aatcgccttg ggctggcccg gcaggttgat gatcaggctc
     9721 ttgccgcgta tcaccgccac ctggcgcgac aggatggccg tggggacgaa tttgagcgag
     9781 atctggcgca tctgctcgcc aaagcccggc atctccttgt cggccacggc cagcgtggcc
     9841 tcgggcgtca cgtcgcgcag cgccgggccg gtgccgccgg tggtcagcac caggctgcag
     9901 ccggcatcca ccagctcgac cagcgtggcg ctgatgcggt ctttttcgtc ggggatcagg
     9961 cgcggctcga aggtcagggg gttgtgcagc gcgcgcttga gccagtcgtg cagcgcgggc
    10021 aggcccttgt cctcgtagtc gccgctgctg gcgcggtcgc tgatggagac gatgccgatc
    10081 ttgaccggct cgtattcatg ggcggtatgg ggcatggccg tcctcctcgt catgtgtgtc
    10141 gggcagggct tcattgtggg cctggccggc caacatttgc gcacgcacca gctgaaacag
    10201 atcgcggtag gcgcggccct tgcgcggcgc caggccctgc gacacggcgg ccttgtcctc
    10261 ggggggggcg tccttgcgcg cctggcggat cagggcgcgg atctgctgcg tgtcggtgtc
    10321 aggaaactgc tgcagccaca gcggcagggc ctcgtcgccg gcaatcaggc ggtcgcgcca
    10381 atgctcggcc tggtgcagcg ccagcttctc ggccgccgat ccgccgtgct gctcgtccag
    10441 cgcggcctgc acggcggcca cggtgtcgtc gccgagcttg cgcatcagct tgccgacaaa
    10501 ctgcatctgc cggcgcttgc cctcgaagtt ggtgatgcgc ttggcctcgg ccagcgcgtc
    10561 ctgcagtttt tcgggcaact ccaggcgcgc aaacaggtcg gcgcgcagcg agagcaggtc
    10621 ggcgcccagc acctgcaggg cctccatctc gcgcttgagc tcggtcttgg tggcgccggt
    10681 cgtgcctttg agttcggcct tgaattgaag gtccatctcg ctgccttcgg caacgaaatg
    10741 gcccttcaca aaatagcctt ttttgggttt gcgtgacatg gtggggcagg gcagaagcag
    10801 tgccgcaatg gcggcggccc tgcggtgatt tgagcggcaa gtatcatagc cgccgctatg
    10861 aatacaccca gctcccgcgc caagagcgcc gcccccgcgc agcaccccac ccagcccgaa
    10921 agcggcttca gctacacccg cgaattcttc gccggcctgg tagaccacgc cctggcccac
    10981 gccaagaagc tgggcgccag cgacgccggg gccgatgcgt cggagggctg cggcctgtcc
    11041 gtcaacgtgc gcaagggcga gctggagacc gtggagcgca accgcgacaa atccctgggc
    11101 gtgaccgtct acctgggcca ccggcgcggc aatgccagca cgtccgactt ctcgtccgcc
    11161 gccgtggagc gcaccgtgca ggccgcctac gacatcgccc gcttcaccgc cgaagacccc
    11221 gtggccggcc tgcccgacga ggccgatatc gccccgcagg acagccaccg cgacctgcag
    11281 ctattccacc cctgggccgt caccagcgaa gaggccgcgc aactggcgct gcaatgcgag
    11341 gccgccgccc tcaagaccca caggcgcatc accaacagcg agggcgcggg cgtgtcggcc
    11401 cagcaaagcc acttcttcag cgcccacacg cgcggctttc gcggcggcta tgccagctcg
    11461 cgccacagca tctcggtggc gccgatcgcc tcgctgcccg ggcgtaatgg cgagatgcag
    11521 cgcgacgcct ggtattcatc catgcgcgac gcgcgtgagc tggccgcgcc cgaggccgtg
    11581 ggccgctacg ctgcccagcg cgccctcgcc cgcctggggt cacgcaagat ccccaccacc
    11641 gaatgcccgg tgctgttcga atcccccctg gccgctggcc tgctgggcag cttcgtgcag
    11701 gccatcagcg gcggcgcgct gtaccgcaag accagctttc tggtcgattc attggacaag
    11761 caggtctttg ccaagcacat caacgtgagc gaagacccct tcatcctggg cggcaagggc
    11821 agctcgccat tcgacgacga gggcgtgcgc gtggcgccgc gccaggtggt caagggcggg
    11881 cgcgtgcagg gctatttctt gagcacctac tcggcacgca agctgggcat gaagaccacg
    11941 ggcaacgccg gcgggtcgca caacctggtg ctgacctcgc gccagactaa agaacacgac
    12001 gacctggacg ccatgctgca aaaactgggc acgggcctgt ttgtggtcga actcatgggc
    12061 cagggcgtga actacgtcac cggcgactac tcgcgcggcg ccagcggctt ctgggtggaa
    12121 aacggccgca tcgcctaccc cgtgcatgag atcaccatcg ccggcaacct caagaccatg
    12181 ctgcgcggca tcgaggccgt gggcgccgac gcctacaact acggcgccaa gaccgtgggc
    12241 tccatcctca tcaaccgcat gaaggtggcg ggtagctgac cgggagcgcc ggcatcctgc
    12301 cggccgaagt cccccggcct ttccccgaac atttagccaa aacagcatgc agcgcccgta
    12361 gatagggcgc aataagctat aaaaagaata tcaaatccgc gtaccaccca tccgctggtt
    12421 tcactccccc cccctctggg ggagggcagg ggtgggggcc agcgacgctc aagcccccat
    12481 gccggcaaga tgccggcgcc cccagcccca tacaacccgc agttagagta aaaataggct
    12541 gaaatccccg ccaaataagc gcaaggcact atgtttttaa tagtgaaaaa gacctatccc
    12601 atcaccccgc ccacaccgcc ctggtacgcc tgagccactc cttgcgatcc cccagcccga
    12661 tcagcccgcc gttgatggcc ttggtcacct gggtgatgga atcggcatcc gccttctcgt
    12721 tgcacccctt ttgctgccag atggccgccg cgaccgtcac gcaccagccg gcgctgatca
    12781 cctcgtcggg tgcctgcaca aagtccggcg catcggcaaa gctgccgcgc agaatctgcg
    12841 tggcccgctg gtagctgtcc ttgcccgtca actgcaacag gccgcgcccg cgataggtaa
    12901 agccatcatc gggcgcggta ttgcccatgc gcttgccata cacctcgttg gccagcttct
    12961 gcgggttgtg cgcaaaggcg gcagggttca gcggccccgt gggctgaaac ctgctgggcc
    13021 acaccttggg caggcgcgcc gcgctgtagt tcaggttctc aaactggatc gtcagcccgc
    13081 cgcactcgtg cagcatctgc gccatgaaat gcgccatgcg caggggcgag tctgaaatcc
    13141 cgaacaaatc cagcaccggc tgcgcattga taaacgcctc gcggtagctg gaccgggcgc
    13201 tgggggccag cgtggcaacg tcatgctccg tgatccgaac cgtcatatcg agcctcctgt
    13261 caacaccgtg gtgaaaggtt gccgggcgcc gcggcgccca aatcctttgg ccgggctttc
    13321 aaatggccgc tggcattgcc gcacaagcca aggcatgtgg gtgcagcatg gcacgcctgc
    13381 tcgggtgctg gcaagcgctt gcacaccagg cgcggcaggc aggcaaagcg ctgctcaatg
    13441 gccaggcgcg atcgctgcta ccgccaagcc gttacctgta gcctccagct ccagttggcc
    13501 ctcaagagtt tcgaatttgg cgataccgac gagcgtttga tagagcgaaa tgagctgagt
    13561 atggaaggac tcaagctcac cggttaggcg ccaccgctcg gaaccggaga tcttggtttg
    13621 agcaacagca ccccgacgat gagtggaccc gcgagaggaa ccagggcgaa gaatgcagca
    13681 acggggtggc gggctctgta gcgggcaagt agggcggagg tgacgatgaa ggcagccaaa
    13741 agaaggatcg aggtgacgat ggaaaacatg ccggcgcccg gtacggacgc gagcagcagc
    13801 ggcccgacgt actgaagtac aagaagggcg agccaagagc catagagacc tgatggcggc
    13861 tcagtgttgg gcacgggaga gggggggctg agcttgtggc gaaagccgag ccagagcaag
    13921 gcaaatgcga tggtgaatac aagcaggcca agcacgactg caaagccaaa ggcgccatag
    13981 ccgcaagcgc cctcgaagga ggagccgaac caactgcatg gtgcgaagac aagcaaagcg
    14041 aagcccgaag caaagccgac gagcaaagca accagaagtg ctgcaccaaa gcgaaagatg
    14101 gaatctatgg acgtctcctt ggaggtttgc tggtgtctaa ccctgagttc agccgacgcc
    14161 gtaggcggtc ggctgcgacg aatggttaga a
//
LOCUS       DAOMTQ010000076        14056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_719_length_14056_cov_26.162060, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000076 DAOMTQ010000000
VERSION     DAOMTQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_719_length_14056_cov_26.162060"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /locus_tag="PK518_08890"
     CDS             complement(<1..1618)
                     /locus_tag="PK518_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM49072.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLLALLDNPSAAEVL
                     RACAANIDDLRASLTNFIKDNTPQVAGTDEVDTQPTLGFQRVIQRAIMHVQSTGNGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNYIAHGIKKGEPPEPPKPEGQAES
                     EEAAQGERGEKASPLEQYTQNLNQAAKDGRIDPLIGRDYEVERTIQILCRRRKNNPLL
                     VGEAGVGKTAIAEGLAWRITEGTVPEILAEAIVYSLDMGALLAGTKYRGDFEQRLKGV
                     LKSLKDKPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGTLKCIGATTFTEYR
                     GIFEKDAALSRRFQKIDVVEPTVAETIDILKGLKTRFEEHHSVKYAAAALQAAAELSA
                     KYINDRHLPDKAIDVIDEAGAAQRILAPSKRKKTIGKSEIEEIVAKIARIPPANVSND
                     DRGKLQTLERDLKSVVFGQDKALEVLAASVKMARSGLGKGDKPIGSFLFSGPTGVGKT
                     EAAKQLAYIMGVDLIRFDMSEYMERHAVSRL"
     gene            complement(1690..2043)
                     /gene="clpS"
                     /locus_tag="PK518_08895"
     CDS             complement(1690..2043)
                     /gene="clpS"
                     /locus_tag="PK518_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRM49073.1"
                     /translation="MATKPPKSAPAPRLPARDDGGSVVLERRTQKTKPPQMYQVVMLN
                     DDYTPMEFVVVVLQEFFNKDREAATQIMLKIHLDGKGICGVYTRDVAATKVEQVLDAA
                     HKAGHPLQCLSEPIE"
     gene            complement(2111..5659)
                     /gene="dnaE"
                     /locus_tag="PK518_08900"
     CDS             complement(2111..5659)
                     /gene="dnaE"
                     /locus_tag="PK518_08900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRM49074.1"
                     /translation="MFVHLRLHTEFSVVDGTTRIDEVVKAAAKDGQPALAITDLNNLF
                     GAIKFYKEGRGKGVKPILGAEVYLEGEAGAPPARLLLLVQNRQGYLNLSELLARAWTR
                     GVVKNLPLCTWDWLQELHAGLIALAGAQAGPVGQALMRGDEAGAAALALRLAGVFPHR
                     FYIELQRAGRADDEAHVTAAVQLAARLQLPVVATHPVQFGQADGYEAHEARVCIAEGE
                     ILANQRRVRRFTREQYFKSSAEMQALFADLPSAIANTLEIAKRCNLTLVLGKPQLPDF
                     PTPNGMPIDEYFRYASFEGLKERLLHLFPQEAEREKQRPRYEERLEFELGTILKMGFP
                     GYFLIVGDFIQWAKANGCPVGPGRGSGAGSLVAYALKITDLDPLQYNLLFERFLNPER
                     VSMPDFDIDFCQSNRDRVIDYVKDKYGKNAVSQIATFGTMAAKAAIRDVGRVMDMSYT
                     FCDGISKLVPGKPGQTYTLAYPPEPKKEGDKNNYALELEPQLYERVRNEEDVRTIIEM
                     AQQLEGMTRNIGMHAGGVLIAPGKLTDFCPLYQQPGSESAVSQYDKDDVEAIGLVKFD
                     FLGLATLTILEIAREFIQQRHKGQENFAFEDIPLDDAPTYRLFSDGKTEAVFQFESRG
                     MQGMLKEARPSRLEDLIALNALYRPGPMDLIPSFVNRKHGKEVVEYPHPLVEPVLAET
                     YGIMVYQEQVMQTAQVLGGYSLGGADMLRRAMGKKKPEEMAEHRIIFRKGAAEKGISQ
                     DKADEVFDLMEKFAGYGFNKSHAAAYSLLAYHTGWLKVHFTAEFFCANMTVEMDDTDK
                     LKVLYEDAVKHFGISFEPPDINRGRYRFEPVTDKVIRYGLGAVKGTGQQAIEAIIAAR
                     EGRGEGPRGGDHGPFKSLFDFCVRVDRARINKRTVEALIKAGAFDALQLNRAALVASI
                     DRAFDFAAAQLANANQGGLFDMMGEDAVGSSTQEPDLADATPWGIKERLMQEKTAVGF
                     YLSGHLFDEVQQEVRRFVRTPIAELGDSRETQVLAGIVSDSRQINGQRGKLALFKIDD
                     KSAVIEASADEAVIAASGAMLKDDEFVVLSGRLQLDHFSGGLRLKVQQVWDLGAARAR
                     FGRYLLVGANGGTRPDVAALVREFPPRREETDQGEMLVHGLRVRMGVHCQGAGGAADA
                     ELQLGEASRFFPSDAALAAWGAQAGSSNISVVYESA"
     gene            5805..6602
                     /locus_tag="PK518_08905"
     CDS             5805..6602
                     /locus_tag="PK518_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRM49075.1"
                     /translation="MPRFPLSLLSALLAAGLLAACASPNEDKTAGWSTDKIYAEARDE
                     LNGGAYDKAVPLLEKLEGRAAGTPLAQQAQLEKAYAQYKGGEKAQAVATLDRFMKLHP
                     ASPAFDYALYLKGLVNFNENLGLFSWLSRQDLSERDQKAAKDSFESFRELATRFPESR
                     YTPDARLRMTYIINSLAQYEVHVARYYYERGAYVAAVGRAQSAIADYKDVPATEEALY
                     ILVRSYDALGMTQLRDDARRVLEASYPNSSLPKAGFKGTDNPWWKFW"
     gene            complement(6565..8610)
                     /locus_tag="PK518_08910"
     CDS             complement(6565..8610)
                     /locus_tag="PK518_08910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRM49076.1"
                     /translation="MSLSDLVAGAFAADGPLARAQPHFRPRAEQTAMAQAVAEAIEDH
                     APLVVEAGTGVGKTFAYLVPALLSGERVVVSTATKALQDQLYGRDLPLLLRALALPLC
                     TALLKGRASYLCHQRLQQARYNPVLAPAQAQELARIELWAQGTQSGDLAELPGLDERS
                     PLLGLVTSTRDNCLGQDCPRFADCHVYQARRTALAADVLVINHHLFFADQAVRESGVA
                     QLLPNSGVVVFDEAHQLAETASQFLGAQLGTGQLHDLAQDLLVSGLRHARGLADWQGL
                     GALLRQATRELHLLVADSAAGVVPGRVAWVGAAPDGVDATAWDGAMARAVGVLRGILA
                     GLDGVTALAPDLAHLHERTVDLLARLARFSAPGSADGVRWLDWTGQLRLSEVPLALAP
                     ALRTLWQGPPAEAGAWDEPVAPLAGCKRTWVFTSATLGDDAQLSWFTEACGMDGARTL
                     RLDSPFDYARQAALYVPPQLPAATDPAHSEQLAHWVADAATRLGGRTLVLTTSLRALQ
                     VMAGVLQQRLAVGGGIEVLVQGQAPKRQIMECFRAPADAVGHVLVGSASFWEGFDVPG
                     QALQLLVIDKLPFPSPGDPLVQARGRRLKQQGRSPFRDDALPAAALALKQGAGRLIRS
                     ESDTGVLVVADQRLLRMGYGKRLLRALPAMRRLRSQEEFDAALAAFTRTSTTGCPCP"
     gene            8705..9511
                     /locus_tag="PK518_08915"
     CDS             8705..9511
                     /locus_tag="PK518_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRM49077.1"
                     /translation="MTKAFRLIASTGIHKGDRDYQQDQVALLSHPRYNGCVLAVVADG
                     MGGRSGGRKASDQVMMTARQLFERYCPDTDDASSLLEHIVHESHIVIRLTAISAEQEP
                     HSTVAAFLINPRGDCHWAHAGDSRIYHFQGGRLLYRTADHSYVQALVDRGELSEAEAS
                     NHPHSNILIGCLGSESDPPVTKHLISQLQPGDVLLACSDGVWHYFSPAELASVLESLT
                     PREATEFLIDKARSRARGGGDNLSLVVVRIEALADEKKLPPLVAPLTLSA"
     gene            complement(9512..10144)
                     /locus_tag="PK518_08920"
     CDS             complement(9512..10144)
                     /locus_tag="PK518_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49078.1"
                     /translation="MLLCLAALLALPGLTLAQDAASGTAAGPQQQRELRRAQIQAERQ
                     ALQAGRSSDEAACYQQFAVHDCLNQVRARVREAENRLRRQELQINDEERREKADERRR
                     SIEERQQEQRQNLQSGEKPPPMRATERGNAQTRAQQAQQRAAEQQHRAAQQAQEQQER
                     QQAEAQRASESRARYDAKQQKARERREQHERDKAQAQASGRKPPAPLPAP"
     gene            complement(10174..11271)
                     /gene="zapE"
                     /locus_tag="PK518_08925"
     CDS             complement(10174..11271)
                     /gene="zapE"
                     /locus_tag="PK518_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HRM49079.1"
                     /translation="MTVRQAYEAELAAKGFASDPAQLRAIDALERCADEWSRYKAKRS
                     NSLKKLINHPDIPKGVYMYGGVGRGKSFLMDCFFNAVPLKRKVRLHFHEFMREVHREL
                     ASLQGTQNPLDALGANIAKRYKLICFDEFHVADVTDAMILHRLLVALFENGVGFVTTS
                     NFKPDELYPDGLHRDRILPAIALLNERMEVVNVDNGTDYRRRTLENARLYHCPLGPQA
                     DAEMAQTFDQLAEVHDEDPVLRIEAREIHALRKAGGVVWFDFRELCMGPRSQNDYLEL
                     ASQFHTVLLSNVPHMPVNMASPARRFTWLVDVLYDRRVKLIISAAVPPEDLYTDGPLA
                     HEFPRTVSRLNEMQSKEYLQLERRVVDTGLT"
     gene            complement(11354..12781)
                     /gene="lpdA"
                     /locus_tag="PK518_08930"
     CDS             complement(11354..12781)
                     /gene="lpdA"
                     /locus_tag="PK518_08930"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRM49080.1"
                     /translation="MSKQFDVVVIGAGPGGYIAAIRAAQLGMNVACIDAWTNDKGGPA
                     PGGTCTNVGCIPSKALLQSSEHFEHANKHFAEHGISTGKVTMDVATMIGRKDTVVKQN
                     NDGILYLFKKNKVSFFHGRGSFVKAVEGGYEIKVAGKTEDLLTAKQIIVATGSNARAL
                     PGVEFDEVNVLSNDGALAIGAVPKKLGVVGSGVIGLEMGSVWRRLGADVTVLEGLPTF
                     LGAVDEQVAKEAKKAFDKQGLKIELGVKIGEVKNGKKGVSVAYTNAKGEAQKLDVDKL
                     IVSIGRVPNTIGLNAEAVGLQLDERGAIVVDGDCRTNLPGVWAVGDVVRGPMLAHKAE
                     EEGVAVAERMAGQHGHVNFNTIPWVIYTSPEIAWVGRTEQQLKADGVKYKAGSFPFLA
                     NGRARALGDTTGFVKFLADAATDEILGVHIVGPMASELIAEAVVAMEFKASAEDIARI
                     CHAHPSLSEATKEAALAVDKRTLNF"
     gene            complement(12858..>14056)
                     /gene="odhB"
                     /locus_tag="PK518_08935"
     CDS             complement(12858..>14056)
                     /gene="odhB"
                     /locus_tag="PK518_08935"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRM49081.1"
                     /translation="QWKKKPGEAVAQDEILIEIETDKVVLEVPAPAAGVMAEHVVGDG
                     GTVVSEQVIAKIDTEGKAGAAAAAPAAAPAAAAAPAAAAAPAAAGGNKGDVAMPAAAK
                     ILAEANLSAAQVAGSGKDGRVTKGDALAAVAGGVKSTAAVIPTGVPTKALPQVAAPAG
                     GLNLGDRPEQRVPMSRLRARVAERLLQSQSTNAILTTFNEVNMAPVMEMRKKFQDAFT
                     KEHGVKIGFMSFFVKAAVHALKKFPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPI
                     LRNADQMSFADIEKKIAEFGKKAAEGKLGIEEMTGGTFSISNGGTFGSMMSTPIINPP
                     QSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLVAMKDALEDPS
                     RLLFDL"
BASE COUNT         2232 a         4722 c         4551 g         2551 t
ORIGIN      
        1 tcaggcggct cacggcatgg cgctccatgt actccgacat gtcgaagcgg atcagatcca
       61 cgcccatgat gtaggccagc tgcttggcgg cctcggtctt gcccacaccg gtggggccgc
      121 tgaacaaaaa gctgccgatc ggcttgtcgc ccttgcccag gcccgagcgc gccatcttca
      181 cgctggcggc cagcacctcc agtgccttgt cctggccaaa caccacgctc ttcaggtcgc
      241 gctccagcgt ctgcagcttg ccgcggtcgt cgttgctgac gttggcgggc gggatgcggg
      301 ctatttttgc aacgatttcc tcgatctcgc tcttgccgat ggttttcttg cgcttggatg
      361 gcgcaagaat gcgctgcgcc gcgccagcct cgtcgatgac gtcgatggcc ttgtcgggta
      421 ggtgccggtc gttgatgtac ttggccgaca gctcggccgc ggcctgcagc gcggcagcgg
      481 cgtacttcac gctgtggtgc tcctcaaagc gggttttcag gcccttgagg atgtcgatgg
      541 tctcggccac cgtgggttcg accacgtcga tcttctggaa acgccgcgac aacgctgcgt
      601 ctttttcgaa gatgcctcgg tattcggtga aggtggttgc gccaatgcac ttgagcgtgc
      661 ccgagctgag tgccggcttg agcaggttgc tcgcgtccag cgttccgccc gacgcggcgc
      721 ccgcgccgat cagcgtgtgg atctcgtcga tgaacaagat ggcgttgggc ttgtccttga
      781 ggctcttgag cacgcctttc aggcgctgct cgaagtcgcc acggtacttg gtgccggcca
      841 gcagcgcgcc catgtccagc gaatacacga tggcctcggc caggatctcg ggcaccgtgc
      901 cttcggtgat tctccaggcc aggccttccg caatggccgt cttgcccacg ccggcttcac
      961 ctaccagcag cgggttgttc ttgcgccggc ggcacaggat ctggatggtg cgctcgacct
     1021 cgtagtcgcg cccgatcagc gggtcgatcc tgccatcttt cgcggcctgg ttgaggtttt
     1081 gcgtgtactg ctccagcggc gaggccttct cgccgcgctc gccctgggcc gcctcttcgc
     1141 tctcggcctg tccctcgggc ttgggcggct cgggcggctc gcccttcttg atgccgtggg
     1201 cgatgtagtt cactacatcc aggcgcgtca caccctgctg gtgcaggtag tacacggcgt
     1261 gcgaatcctt ctcgccaaag atggccacga gcacgttggc gcccgtgact tccttcttgc
     1321 cgttgccggt ggactgcacg tgcatgatgg cgcgctggat gacgcgctgg aaacccagcg
     1381 tgggctgggt gtccacctcg tcggtgccgg ccacctgggg ggtgttgtcc ttgatgaaat
     1441 tcgtcagcga tgcacgcagg tcatcgatgt tggccgcgca ggcgcgcagc acctcggcgg
     1501 cgctggggtt gtccagcagc gccagcagca ggtgctccac ggtaatgaac tcgtggcgct
     1561 gctggcgggc ctcgacgaag gccatgtgca agctgacttc cagttcttgg gcaatcatgt
     1621 gagaactcct atgtgcttgc gtggtaaggg tgactttaga tcgggggcaa aggccattta
     1681 ttcaacaggc tattcaatcg gctcgctcag gcattgcagc gggtggccag ccttgtgggc
     1741 cgcgtccagc acctgctcca ccttggtggc ggccacgtcg cgcgtataca cgccgcagat
     1801 gcctttgccg tccaggtgga tcttgagcat gatctgcgtc gcggcctcgc gatccttgtt
     1861 gaagaactcc tgcagcacga cgaccacgaa ctccatcggc gtgtagtcgt cgttgagcat
     1921 gaccacctgg tacatctgtg gcggcttggt cttctgggta cgccgctcga gcacgaccga
     1981 gccgccgtcg tccctggcgg gcaggcgtgg cgcgggcgcg gacttagggg gtttggttgc
     2041 catgtgtttc attctagtgc gccatgtgtt cgcgcagcag tcccagtaac tggtaccaaa
     2101 gccggccttt tcaagcgctc tcgtacacca cgctgatatt gctgctaccg gcctgcgcgc
     2161 cccaggcggc cagagccgcg tcgctgggaa agaagcggct ggcctcgccc agttgcaact
     2221 cggcgtccgc ggcaccaccc gccccctggc aatgcacgcc catgcgcacg cgcaggccgt
     2281 gcaccagcat ctcgccttgg tcggtttcct cgcgtcgcgg gggaaattca cgcaccaggg
     2341 ccgccacgtc gggcctggtg ccgccattgg cgcccaccag caggtagcgg ccaaagcgtg
     2401 cgcgcgccgc gcccaggtcc cagacctgct gcaccttcag gcgcagcccg ccgctgaagt
     2461 gatcgagctg cagccgcccg ctcaagacca caaactcgtc gtccttgagc atggcgccgc
     2521 tggcggcgat cactgcctcg tccgccgagg cctcgatcac cgccgacttg tcgtcgatct
     2581 tgaacagcgc cagcttgccg cgctggccat tgatctggcg cgagtcgctg acgatgccgg
     2641 ccagcacctg ggtctcgcgg ctgtcgccca gctcggcaat cggcgtgcgc acaaagcgcc
     2701 gcacctcctg ctgcacctcg tcgaacagat ggcccgagag gtagaagccg accgccgtct
     2761 tttcctgcat caggcgctcc ttgatgcccc agggcgtggc gtcggccaga tcgggctcct
     2821 gggtgctgga gcccacggcg tcctcgccca tcatgtcgaa caacccgccc tggttggcgt
     2881 tggccagttg cgctgcggca aagtcaaatg cgcggtctat ggacgccacc agcgccgcac
     2941 ggttgagctg cagcgcgtcg aaggcgccgg ccttgatcag cgcctccacc gtgcgcttgt
     3001 tgatgcgcgc cctgtccacg cgcacgcaga agtcgaacaa gctcttgaac ggcccgtggt
     3061 cgccgccgcg cggcccctcg ccacggccct cacgcgcggc aatgatggcc tctatggcct
     3121 gctggcccgt gcccttgacg gcgcccaggc cgtagcggat gaccttgtcg gtcacgggct
     3181 cgaagcggta cctgccgcgg ttgatgtccg gcggctcgaa gctgatgccg aaatgcttga
     3241 ccgcatcctc gtacagcacc ttgagcttgt cggtgtcgtc catttccacc gtcatgttgg
     3301 cgcagaagaa ctcggccgtg aagtgcacct tgagccagcc cgtgtggtag gccagcagcg
     3361 aataggcggc ggcatgcgac ttgttgaagc cgtagccggc aaacttctcc atcaggtcga
     3421 agacctcgtc ggccttgtcc tggctgatgc ccttctctgc cgcgcccttg cggaagatga
     3481 tgcggtgctc ggccatttcc tcgggctttt tcttgcccat ggcgcggcgc aacatgtcgg
     3541 cgccgcccag gctgtagccg cccagcacct gggcggtctg catcacctgc tcctggtaga
     3601 ccatgatgcc gtaggtctcg gccagcacgg gctcgaccag cgggtgcggg tactccacca
     3661 cctccttgcc gtgcttgcgg ttcacgaagc tgggaatcag gtccatgggg cccgggcggt
     3721 acagggcgtt gagggcaatc aggtcttcca ggcggctggg gcgggcctct ttcagcatgc
     3781 cctgcatgcc gcggctttca aactggaaca ccgcctcggt cttgccgtcg gagaacagcc
     3841 ggtaggtggg cgcgtcatcc agcgggatgt cctcgaaggc aaagttctcc tggcctttgt
     3901 ggcgctgctg tatgaattcg cgtgcaatct ccaggatggt gagcgtcgcc aggcccagaa
     3961 agtcgaactt gaccaggcct atggcctcga catcgtcctt gtcgtactgg ctcacggccg
     4021 actcgctgcc tggctgctgg tagagcgggc aaaaatcggt cagcttgccc ggtgcaatca
     4081 gcacgccgcc ggcgtgcatg ccgatgttgc gcgtcatgcc ctcgagctgc tgcgccatct
     4141 cgatgatggt gcgcacatcc tcctcgttgc gcacgcgctc atacagctgg ggttcgagct
     4201 ccagcgcgta gttgttcttg tcgccttcct tcttgggctc gggcgggtag gccagggtgt
     4261 aggtctgacc gggcttgccg ggcaccaatt ttgaaatgcc gtcgcagaag gtgtagctca
     4321 tgtccatgac gcggcccacg tcgcggatgg ccgccttggc ggccatggtg ccgaaggtgg
     4381 cgatctggct cacggcattc ttgccgtact tgtccttcac atagtcgatc acccggtcgc
     4441 ggttggactg gcagaagtcg atgtcgaagt cgggcatgga cacccgctcg gggttcaaaa
     4501 accgctcgaa cagcaggttg tactgcagcg ggtccagatc cgtgatcttc agcgcatagg
     4561 ccaccagcga gcccgcgccc gagccacggc cggggcccac cgggcagccg ttggccttgg
     4621 cccactggat gaagtcgccc acgatgagga agtagcccgg aaagcccatc ttcagaatgg
     4681 tgcccagctc gaactccagc cgttcctcgt agcgcggacg ctgcttttcg cgctcggcct
     4741 cctgggggaa aaggtgcagc aggcgctcct tgaggccctc gaacgaggcg tagcggaaat
     4801 actcatcgat cggcatgcca ttgggcgtgg gaaagtcggg cagctgcggc ttgcccagca
     4861 ccagcgtcag gttgcagcgc ttggcgatct ccagcgtgtt ggcaatggcc gagggcaggt
     4921 cggcaaacag cgcctgcatc tcggcgctgc tcttgaagta ctgctcgcgc gtgaaacggc
     4981 gcacgcgccg ctggttggcc aggatctcgc cctcggcgat gcacacgcgc gcctcatgcg
     5041 cctcgtaacc atcggcctgg ccaaactgca ctgggtgcgt ggccaccacc ggcagctgca
     5101 gccgcgccgc cagctgcacg gcggcggtga catgggcctc gtcatcggca cggccggcgc
     5161 gctgcaactc gatgtaaaaa cgatgtggaa aaacacccgc caggcgcagc gccagcgccg
     5221 ccgcgccggc ttcgtcgcca cgcatcagcg cctggcccac ggggccggcc tgggcgccgg
     5281 ccagcgcgat caggccggca tggagctcct gcagccagtc ccaggtacac agcggcaggt
     5341 tcttcacgac accccgcgtc caggcgcgtg ccagcagctc ggacaggttc aggtagccct
     5401 ggcggttctg caccagcagc agcaggcgcg cgggcggcgc gccggcctcg ccttccaagt
     5461 agacctcggc gcccagaatg ggcttgaccc ccttgccacg cccttccttg tagaacttga
     5521 tggcgccaaa gaggttgttc aggtcggtaa tcgccagggc gggctgccca tccttggcag
     5581 cggccttgac gacctcgtcg atgcgggtgg ttccgtccac gacggaaaat tcggtatgca
     5641 ggcgcaggtg aacaaacatg ccggcattgt aggaatgcgg gcttggcctc gtgctccggc
     5701 aggtcgggcg gcttacttac aatgccgggt tgcgggtacc gcgcggtctt gtctcccgac
     5761 agggttgcca cccatgccat tcattcatcc cgagagctgc cgtcatgccg cgttttcctc
     5821 tatctttgtt gtctgccctg ctggccgccg gccttttggc cgcctgcgcc agccctaacg
     5881 aagacaaaac cgccggctgg agcacggaca aaatctacgc cgaggcgcgc gatgaactca
     5941 acggcggcgc ctatgacaag gccgtgccgc tgctggaaaa gctcgaaggc cgcgccgccg
     6001 gaacgccact ggcccagcag gcgcagctgg aaaaagccta cgcccagtac aagggtggcg
     6061 aaaaggccca ggccgtggcc acgctggatc gcttcatgaa gctgcacccg gcaagccccg
     6121 ccttcgacta cgcgctgtac ctgaagggtc tggtcaactt caatgaaaac ctgggtctgt
     6181 tctcctggct gtcacgccag gacctgtccg agcgcgacca gaaggctgcc aaggattcgt
     6241 tcgaatcctt ccgcgagctg gcgacgcgct ttcccgagtc gcgctacacg ccggatgcgc
     6301 gcctgcgcat gacctacatc atcaactcgc tggcgcagta cgaggtgcat gtcgcccgct
     6361 actactacga gcgcggcgcc tatgtggcgg ccgtgggccg ggcgcaaagc gcgattgcgg
     6421 actacaagga cgtgcccgcg accgaggagg cgctgtacat cctggtgcgt tcctacgacg
     6481 ccctgggcat gacgcagttg cgcgacgacg cgcgccgtgt gctggaagcc tcgtacccga
     6541 acagcagcct gcccaaggca ggcttcaagg gcacggacaa cccgtggtgg aagttctggt
     6601 aaacgccgcc agcgccgcat caaactcttc ttgcgaccgc agcctgcgca tggcgggcag
     6661 ggcccgcagc aggcgcttgc cataacccat gcgcagcagc cgctggtcgg ccacaaccag
     6721 cacaccggta tcgctttcgc tgcgtatcag gcggccggcg ccctgcttga gggccagcgc
     6781 cgccgcaggc aaggcatcgt cacgaaatgg gctgcgcccc tgctgtttga ggcgccgccc
     6841 ccgcgcctgc accagcggat caccggggga cgggaacggc aacttgtcga tcaccagaag
     6901 ctgcagcgcc tggcccggca cgtcaaaccc ctcccagaag ctggccgagc caaccagcac
     6961 atggcccaca gcatccgccg gcgcacgaaa gcattccatg atctgccgct tgggcgcctg
     7021 gccctgcacc agcacctcga tgccaccacc gaccgccagg cgctgctgca gcacgccggc
     7081 catgacctgc agcgcacgca ggctggtggt cagcaccagc gtgcgcccgc ccaggcgcgt
     7141 cgccgcatcc gccacccaat gggccagctg ctcgctgtgc gcgggatccg tggcggcggg
     7201 cagctggggc ggcacataga gcgcggcctg gcgcgcatag tcgaacgggc tgtccagacg
     7261 cagcgtgcgt gcgccatcca tgccgcaggc ctcggtgaac cagctcagtt gtgcgtcatc
     7321 acccagcgtg gccgaggtga acacccaggt gcgcttgcac cccgccaatg gcgccacggg
     7381 ctcgtcccag gctccagcct cggccggcgg gccttgccat agcgtgcgca gggccggggc
     7441 cagagccagc ggcacctcgc tcagccgcag ctggccggtc caatcgagcc agcgcacgcc
     7501 atcggcacta cccggagcgg aaaaccgtgc cagccgcgcc agcaagtcca ccgtgcgttc
     7561 atgcagatgt gccaggtcgg gagccagcgc cgtcacgcca tccagccccg ccaggatgcc
     7621 gcgcaggacg cccactgcac gcgccattgc gccatcccac gccgtggcat ccacgccatc
     7681 gggcgccgca cccacccagg ccacccgccc gggcacgaca cccgcagcac tatcggccac
     7741 aagcaggtgc aactctcgcg tcgcctggcg cagcaaggcg ccgagcccct gccagtcggc
     7801 caatccccgc gcatggcgca ggccgctgac gagcaggtcc tgcgccagat cgtgcagctg
     7861 gccggtgccc aactgtgcgc ccaggaactg gctggcggtc tcggccagtt gatgggcctc
     7921 gtcaaaaacc accacacccg agttgggcaa cagttgcgcc acgcccgatt cgcgcacggc
     7981 ctggtcggca aagaacaggt ggtggttgat caccagcaca tcggccgcca gggccgtgcg
     8041 tcgcgcctgg tacacatggc agtcggcaaa gcgtgggcag tcctggccca gacagttgtc
     8101 gcgcgttgac gtaaccagcc ccaggagcgg cgagcgctca tccagcccgg gcaactccgc
     8161 gaggtcgccg ctctgcgtgc cctgggccca cagctcgatg cgcgccaatt cttgcgcctg
     8221 ggccggggcc agcaccgggt tgtatcgtgc ctgctgcagg cgttggtggc acaggtagct
     8281 ggcccgcccc ttgagcaagg ccgtacacag cggcagtgcc agggcgcgca gcaacagcgg
     8341 caggtcacgg ccatacagct gatcctgcag cgccttggtg gccgtggaga ccaccacccg
     8401 ctcaccgctg agcagggcag gcaccaggta ggcaaaggtc ttgccgacgc cggtgccggc
     8461 ctccaccacc agcggggcgt ggtcttcgat ggcctctgcc accgcctgcg ccatggccgt
     8521 ctgctcggcg cgcggccgaa aatgcggctg cgcgcgcgcc agcggcccat cggccgcgaa
     8581 cgcgccggcg acgagatcac tcaaggacat gcgagaaacg gaacccggac ggcatggcgt
     8641 tgataataat gggctcccag ccgccaccgc cccccgcggg cgccccagcg cccaaccgca
     8701 gcccatgacc aaagcgttcc gcctcattgc ttcgaccggc atccacaagg gtgaccgcga
     8761 ctaccagcag gatcaggtcg ccctgctctc gcatccgcgc tacaacggct gtgtgctggc
     8821 ggtggtggcc gatggcatgg gtggacgcag cggcgggcgc aaggcctcgg atcaggtgat
     8881 gatgaccgcg cgccagctgt tcgagcgcta ttgccccgac accgacgacg ccagcagcct
     8941 gcttgagcac atcgtgcacg agtcgcatat cgtcattcgc ctcacggcca tatcggccga
     9001 gcaggagccg cacagcacgg tggccgcctt tttgatcaac ccgcgcggag actgccattg
     9061 ggcgcatgcc ggggattcac gcatttacca cttccagggc gggcggctgc tctaccgcac
     9121 ggcggatcac tcctatgtgc aagccctggt cgatcgcggc gagctgagcg aggccgaggc
     9181 cagcaaccac ccgcactcca acatcctcat aggctgcctg ggctcggaaa gcgacccgcc
     9241 cgtgaccaag catctgatct cccagctgca gccgggcgat gtgctgctgg cctgcagcga
     9301 tggcgtgtgg cattactttt cgccggccga actggcctcg gtgctggagt cgctgacacc
     9361 gcgcgaggcc accgagttcc tgatcgacaa ggcccgctcc cgcgcgcgtg gcggtggcga
     9421 caacctgtcg ctggtggtgg tgaggatcga ggccctggca gacgagaaga agcttccccc
     9481 cctggtggcg ccgctgacgc tctcggcctg atcagggggc aggcagcggg gccggtggct
     9541 tgcgccccga tgcctgtgcc tgggccttgt cgcgctcatg ctgctcgcgc cgctcacgcg
     9601 ccttttgctg cttcgcgtcg tagcgtgcgc gtgattccga tgcgcgctgc gcctcggcct
     9661 gctggcgctc ctgctgctct tgcgcctgct gcgcggcgcg gtgctgctgc tcggcggcgc
     9721 gctgctgggc ctgctgcgcg cgcgtttgcg cattgccgcg ctccgtggcg cgcatcggcg
     9781 gcggtttttc tcccgactgc aggttctgcc gctgctcctg ctgccgctcc tcgatggagc
     9841 ggcgccgctc gtcggccttt tcccggcgct cttcgtcgtt gatttgcagc tcctggcggc
     9901 gcagccgatt ctcggcctcg cgcaccctgg cgcggacctg gttcaggcag tcatgcacgg
     9961 caaactgctg gtagcaggcc gcctcatcgc tgctgcgccc ggcctgcagg gcctggcgct
    10021 cggcctgtat ctgcgcgcgc cgcaattcgc gctgctgctg tggcccggcg gcagtgccgg
    10081 aagccgcatc ctgcgccaga gtgaggccgg gcaatgccag cagcgcggcc aggcacagaa
    10141 gcaccctcag ggaatgacgg gcatggatgg gcatcaggtc agtccggtat cgacgacacg
    10201 ccgctcaagc tgcaggtatt ccttggactg catctcgttg aggcgcgaga ccgtgcgcgg
    10261 aaactcgtgc gccagcgggc cgtcggtata caagtcctcg ggcggcaccg ccgccgaaat
    10321 gatgagcttg acgcgtctgt cgtacaacac gtccaccagc caggtgaagc gccttgccgg
    10381 cgaggccatg ttgaccggca tatgcggcac gttcgacagc agcacggtgt ggaactggct
    10441 ggccagctcc agatagtcgt tctgcgagcg cgggcccatg cacagttcgc gaaagtcgaa
    10501 ccacaccacg ccgccggcct tgcgcagggc gtggatctcg cgcgcctcta tgcgcagcac
    10561 cggatcttcg tcatgcacct ccgccagctg gtcgaaggtt tgcgccatct cggcgtcggc
    10621 ctgcggcccc agcgggcagt ggtagagcct ggcgttctcc agcgtgcggc ggcggtagtc
    10681 ggtaccgttg tcgacattca ccacctccat gcgctcgttc aagagcgcaa tcgccggcag
    10741 gatgcggtcg cggtgcaggc cgtcggggta gagctcgtcc ggcttgaagt tggaggtggt
    10801 gacaaagccc acgccgtttt cgaacaacgc caccagcagg cgatgcagga tcatggcgtc
    10861 ggtcacgtcc gctacgtgaa attcatcgaa gcaaatcagc ttgtagcgct tggcgatgtt
    10921 ggcgcccagg gcgtccagcg ggttttgcgt gccctgcagg ctggcgagct cgcggtgcac
    10981 ctcgcgcatg aactcgtgaa agtgcaggcg caccttgcgt ttcaggggca ccgcgttgaa
    11041 gaagcaatcc atgagaaagc tcttgccccg cccgacgccg ccgtacatgt agacgccctt
    11101 gggaatgtcg gggtggttga tgagcttctt caaactgttg gagcgcttgg ccttgtagcg
    11161 gctccattcg tcggcgcagc gctccagcgc atcgatggcg cgcagctgcg ccgggtcgct
    11221 tgcaaatccc ttggcggcca gctccgcctc gtacgcctgc ctgactgtca ccgcattctc
    11281 cgtatactgc aaaaataata gctgctagcg cttgatagtt aagcgctagc agctattttc
    11341 acctaaattt tacttagaaa ttcagggtgc gcttgtccac cgccagggcg gcttccttgg
    11401 tggcctcgct caaagagggg tgggcgtggc aaatgcgggc gatgtcctcg gcgctggcct
    11461 tgaactccat cgccaccacg gcctcggcga tcagctcgga cgccatgggg ccgacgatgt
    11521 gcacgccgag gatttcgtcg gtcgccgcat cggccaggaa cttgacgaaa ccggtggtgt
    11581 cgcccagggc gcgcgcgcgg ccgttggcca ggaagggaaa gctgccggcc ttgtacttga
    11641 cgccgtcggc tttgagttgc tgctcggtgc ggccgaccca ggcgatctca gggctggtgt
    11701 agatgaccca ggggatggtg ttgaagttga catggccgtg ctggcccgcc atgcgctcgg
    11761 ccacggccac gccttcttcc tcggccttgt gcgccagcat ggggccacgc accacgtcgc
    11821 ccacggccca gacgccgggc agattcgtcc ggcaatcgcc atcgaccacg atcgcgccgc
    11881 gctcgtccag ctgcaggccc acggcctcgg cattcaggcc gatcgtgttg ggtacgcggc
    11941 caatggagac gatgagcttg tccacgtcga gcttttgcgc ttcgcccttg gcattggtgt
    12001 aggcgacgga cacacccttc ttgccgttct tcacctcgcc aatcttcaca cccagctcga
    12061 tcttcaggcc ctgcttgtca aaggccttct tggcctcctt ggcgacctgc tcgtcgaccg
    12121 cgcccaggaa ggtcggcagg ccttcgagca ccgtcacgtc ggcgcccagg cggcgccaga
    12181 cgctgcccat ctccaggccg atgacgcccg aacccaccac gccgagcttc ttgggcacgg
    12241 cgccaatagc cagcgcgccg tcgttagaca gcacgttgac ctcgtcgaac tccacgccgg
    12301 gcagcgcacg ggcgttggag ccggtggcga cgatgatctg cttggcggtc agcagatctt
    12361 cggtcttgcc ggccaccttg atctcgtagc cgccttcaac ggccttgacg aacgagccgc
    12421 ggccgtggaa gaagctgacc ttgttcttct tgaacaggta gaggatgccg tcgttgttct
    12481 gcttcaccac ggtgtccttg cggccgatca tggtggccac gtccatcgtc accttgccgg
    12541 tgctgatacc gtgctcggca aaatgcttgt tggcgtgctc gaaatgctcg ctcgactgca
    12601 gcagcgcctt ggacgggatg cagcccacgt tggtgcaggt gccaccgggc gcgggaccgc
    12661 ccttgtcgtt ggtccaggcg tcgatacagg cgacgttcat gcccaactgg gcggcgcgga
    12721 tggcggcgat atagccgccg ggaccggcgc cgatgacgac aacgtcaaat tgtttgctca
    12781 tggtgtgaat tcttgaaaaa gagttgaaaa aacccaccgc tgggctgggc cgagggtggg
    12841 tttcaggagg ccttgaatta caggtcgaac aagagacgcg aggggtcttc cagcgcgtcc
    12901 ttcatcgcca ccaggcccag cacggcctcg cggccgtcga tgatgcggtg gtcatacgac
    12961 atggccaggt agttcatcgg gcgcaccacg acctggccgt tttcgaccac ggcgcgatcc
    13021 ttggtggcat gcacgcccag gatggccgac tgcggcgggt tgatgatggg cgtggacatc
    13081 atcgagccga aggtgccgcc gttggaaatc gagaaggtgc cgccggtcat ctcctcgatg
    13141 cccagcttgc cctcggcggc cttcttgccg aattcggcaa tcttcttctc aatgtcggca
    13201 aagctcatct ggtcagcatt gcgcagaatc ggcaccacca ggccgcgcgg gctgcccacg
    13261 gcgatgccga tgtcgaagta gccgtgatag acgatgtcgt tgccgtcaac ggaagcgttc
    13321 agcaccggga atttcttcag ggcgtgcacg gcggccttga cgaagaagga catgaagccg
    13381 atcttcacgc cgtgttcctt ggtgaaggcg tcctggaact tcttgcgcat ctccatcacc
    13441 ggggccatgt tcacttcgtt gaaggtggtc aggatggcgt tggtggactg cgactgcagc
    13501 aggcgctcgg ccacgcgggc gcgcaggcgg ctcatgggca cgcgctgctc ggggcgatcg
    13561 cccaggttca ggccgccagc aggcgctgcc acctgcggca gtgccttggt gggcacgccg
    13621 gtggggatga cggcggcggt ggacttgacg ccaccggcga cggcggccag ggcgtcgccc
    13681 ttggtcacgc ggccatcctt gccggagcct gccacctggg cggcagacag attggcctcg
    13741 gccaggatct tggcggcggc gggcatggcc acgtcgccct tgttgccgcc agcggcagcc
    13801 ggcgctgcgg cggccgcggg tgctgcggct gcggcaggag cggcagcagg ggccgcagcg
    13861 gctgcgccgg ccttgccttc ggtatcgatc ttggcgatga cctgctcgga gaccaccgtg
    13921 ccaccatcgc cgaccacatg ctcggccatc acgccggcgg ccggggcggg cacttcgagc
    13981 accaccttgt cggtctcgat ctcgatcagg atctcatcct gggcgacggc ctcgccgggc
    14041 tttttcttcc actgca
//
LOCUS       DAOMTQ010000077        13793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_746_length_13793_cov_29.193114, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000077 DAOMTQ010000000
VERSION     DAOMTQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_13793_cov_29.193114"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3437
                     /gene="rpoB"
                     /locus_tag="PK518_08940"
     CDS             <1..3437
                     /gene="rpoB"
                     /locus_tag="PK518_08940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRM49082.1"
                     /translation="ILANFFVNDNFRLMDSGAQLEFVSERLRGEVARFDITDKSGKVI
                     VAKDKRVTARHTRELEQSGTTHISVPEDFLIGRVVARNIVDPDTGEILAKANEELTEA
                     LLKKLRSAGVQELQAIYTNELDQGAYISQTLRIDETVDEFAARVAIYRMMRPGEPPTE
                     DAVQALFQRLFYNPDTYDLSRVGRMKFNAKVGRDEATGPMVLSNDDILSVVKILVDLR
                     NGKGEVDDIDHLGNRRVRCVGELAENQYRTGLARIEKAVKERLGQAEQEPLMPHDLIN
                     SKPISAALKEFFGASQLSQFMDQTNPLAEITHKRRVSALGPGGLTRERAGFEVRDVHV
                     THYGRVCPIETPEGPNIGLINSLALYARLNEYGFIETPYRRVVDGKVTMEIDYLSAIE
                     EGKYIIAQANATLDKDGKLTGDLVSAREKGESTLVSAERVEYMDVSPAQIVSVAASLV
                     PFLEHDDANRALMGANMSRQAVPVLRPEKPMVGTGIERVAAVDSGTVVTARRGGVVDY
                     VDATRIVIRVNDSEAVAGEVGVDIYNLIKYQRSNQNTNIHQRPIVKRGDKLAKGDVIA
                     DGASTDLGEIAIGQNMLIAFMPWNGYNFEDSILISERVVAEDRYTSIHIEELVVMARD
                     TKLGAEEITRDIPNLSEQQLNRLDESGIIYVGAEVQPGDTLVGKVTPKGETTLTPEEK
                     LLRAIFGEKATDVKDTSLRVDQGSQGTVIDVQVFTREGIQRDKRAQQIIDDELKRFRL
                     DLNDQLRIVEADAFDRIEKLLVDKVANGGPQKLAKGTKIDKAYLDGVEKFHWFDIRPA
                     DEAVAAQLESMKNAMEQTRHSFDLAFEEKRKKLTQGDELPAGVLKMVKVYLAVKRRLQ
                     PGDKMAGRHGNKGVVSKIVPVEDMPYMADGTPADIVLNPLGVPSRMNIGQVLEVHLGW
                     AGKGMGQRIGNMLQEQAKAAELRKFFEEIYNSRGRKEELSQLSDDEVMAMARELTTGV
                     PFASPVFDGASEAEIKDMLKIAYPDDIKERKGLTDTRTQAYLYDGRTGERFERPTTIG
                     YMHYLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYV
                     LQEMLTVKSDDVQGRTKVYESIVKGEHAIEAGMPESFNVLVKEIRSLGLDIELERS"
     gene            3569..7798
                     /gene="rpoC"
                     /locus_tag="PK518_08945"
     CDS             3569..7798
                     /gene="rpoC"
                     /locus_tag="PK518_08945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRM49083.1"
                     /translation="MKSLLDLFKQFTPDEHFDAIKIGMASPEKIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIDLAAPCAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDPGMTPLKKFSIM
                     SEDDYDAKRKEYGDEFIAKMGAEGIKELLEGIDLEIEIERLRGDLTGSEVKVKKNAKR
                     LKVLEAFKKSGIKPEWMVLEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNSR
                     LRRLLELKAPEIIARNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADMIKGKSG
                     RFRQNLLGKRVDYSGRSVITVGPTLKLHQCGLPKLMALELFKPFIFSRLEAMGIATTI
                     KAAKKEVEAGTPVVWDILEEVIKEHPVMLNRAPTLHRLGIQAFEPILIEGKAIQLHPL
                     VCAAFNADFDGDQMAVHVPLSVEAQLEARTLMLASNNVLFPASGEPSIVPSQDVVLGL
                     YHATREKINGKGEGMVFTDIGEVQRALDAGEVELATKISVRLTEWNKNKDTGEFEPET
                     KLVETTVGRALLSEILPKGLAFSNLNKALKKKEISKLINASFRKCGLKETVVFADKLL
                     QNGFRLATRAGISICVDDMLVPAQKTDIIGRAEKEVKEIEQQYVSGLVTAGERYNKVV
                     DIWGKAGDDISKVMMDHLKKEKTIDRHGNEVDQESFNSIYMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVTQDLVVTEDDCGTSNGSLMRAIVEGGEVIESLRERILGRVVAEDVLHPETRDVL
                     VAAGRMLDEDTIEEIETVGVDEVKVRTALTCATRYGLCAKCYGRDLGRGGLINLGEAV
                     GVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVEAKSNGIIGFNATMRYVTNTKGE
                     LVVIARSGEIVIQDEHGRERERHKVPYGATLSVKADQQIKAGTILANWDPLTRPIITE
                     FAGTTKFENIEEGLTVAKQVDDVTGLSTLVVIDPKRRGSAKVVRPLVKLIDANGQEVK
                     IPGTDHAVAIGFQIGALIQVRDGQDVGPGEVLARIPVEGQKTRDITGGLPRVAELFEA
                     RSPKDKGMLAEMTGTISFGKETKGKVRLQITDPDGHVWDELVPKEKNVLVHEGQVVNK
                     GELIVDGSADPQDILRLLGIEELSRYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRRV
                     VVENPGETSYIQGEQVERSEILNTNEQAQKDGKIPATYSNVLLGITKASLSTDSFISA
                     ASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGLAYHQARKVKDAMDEAER
                     RAIAEAEAAELASANAEGAAAELDGSADLAD"
     gene            8060..8611
                     /locus_tag="PK518_08950"
     CDS             8060..8611
                     /locus_tag="PK518_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRM49084.1"
                     /translation="MWISWVFLALLLFWAVGAYNRLIRLRSAALQAFGALDAHWQRWI
                     ALLAQYAASRIEATEDDASAGGAADTQAALDAAAAQFAASLAVARARPLDGDAAATLS
                     AAAQVLDTAWQSLAHEAARAGEGVAPPALAPWIHQREQLALHAGEARRLFNEAVGQYN
                     HAIGQFPANLLAWLFGMKKGRAL"
     gene            8608..9978
                     /gene="rsmB"
                     /locus_tag="PK518_08955"
     CDS             8608..9978
                     /gene="rsmB"
                     /locus_tag="PK518_08955"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRM49085.1"
                     /translation="MTTTKPSAPGGAASPPLSRLLDATATALQAIRAGQSGTAALDAS
                     DAALRPAVQALLFQVLRNLGRAQALRRQLAPRNPPARVDALLCTALALAWDTEAAPYE
                     PFTLVNQAVHAAKHSAATRAQAGFVNACLRRFLREREALVVATQGDEVAVWNHPAWWI
                     KRLRQDHPERWQQILQASNLPAPMVLRVSKKKSSPALYQQTLTAIKIESTLVGDSGLA
                     LARPVPVGQLPGFADGVVSVQDGAAQMAAPLLLAGLDASQPLRLLDACAAPGGKTAHL
                     LEWAAALRAPWQVTALEVDAQRAERIHDNLRRLGLSAQVVVADAARPADWWQQASGGA
                     LFDAILLDAPCTASGIVRRHPDVRWLRRESDVAQLAGQQARLLAALWPLLRPGGRMLY
                     CTCSVFRAEGDEQITAFLAHNTDALLLPSPGHLFPGNPDMRHALPDNGPGEHDGFFYA
                     LLQKKT"
     gene            9998..10594
                     /locus_tag="PK518_08960"
     CDS             9998..10594
                     /locus_tag="PK518_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HRM49086.1"
                     /translation="MRWCAALACLLALCWYAPAAQAQQHGEVSDLQLERGADGLYLSA
                     ALRLELPTIVQDALYKGISMHFIADAEVLRPRWYWTDKVVAHATRYVRLSYQPLTRRW
                     RLAQSAAPFAATGLGVSLDQSFDDLPEVLAALERIARWKIAEEGVIDEGQSYLVNFQF
                     RLDMSQLPQPLQIGAVGGSSWNIALARGARLSAQEATR"
     gene            10597..12879
                     /locus_tag="PK518_08965"
     CDS             10597..12879
                     /locus_tag="PK518_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM49087.1"
                     /translation="MGEQRPAGSARRAANRARALRWAVGLGAGVMGAIGLVLLFLLTL
                     ATNNRALYERNFAWLLGVNVLVAVALLAVLVWGAVRLGVRVRRGRFGSHLLIKLAAIF
                     GLVGVVPGLLIYVVSYQFVSRSIESWFDVKVEGALTAGVSLARVTLDTIASDLATKTR
                     GASSQLAQVPDAAAGVVLERMRDQLGASDMVLWNAAGQPVAGVGQSRYSLNPDRPSAQ
                     QLRAARQERISFQIEGLDDINDPMAAENARVRVLVLVVHPGVGLLAEPRYLQATLPLP
                     QALVANAIAVQEANREYQERALARGGLRRMYIGTLTLALFLAVFGAVLLAVVLGNQLA
                     RPLIVLADGVREVAAGNLAPKAALQGKDELGGLTRSFALMTQQLADARSAVERSMEQL
                     DAARGNLQTILDNLTAGVIVLDQQGVIQSSNPGATRILRAPLAAHEGKPLAAVPGLAE
                     FAATVQQHFDAFSLDHGHHSLDHWQHPFELHGSGGGMHAQGTSLVARGAELPNHARLL
                     VFDDISEIVSAQRAQAWGEVARRLAHEIKNPLTPIQLSAERLELKLTGKLPDAEQAVL
                     IKSVKTIVDQVDAMKRLVNEFRDYARLPAAVLQPLDLNALIADVLHLYGAETAQVPVQ
                     AELDVRCPRILGDAQQLRQVVHNLLQNAQDATLQAAEQQKLPPAPVVIATRWSESAQR
                     VRLTVSDCGPGFPAYILQRAFEPYVTTKARGTGLGLAVVKKIADEHGARIDLANRIEG
                     EEMRGAQVSLSFAPEAAVAL"
     gene            12919..13605
                     /locus_tag="PK518_08970"
     CDS             12919..13605
                     /locus_tag="PK518_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM49088.1"
                     /translation="MANILVVDDELGIRDLLSEILNDEGHSVDLAENATQARSARQAN
                     SYDLVLLDIWMPDTDGVSLLKEWAMAGTLTMPVIMMSGHATIDTAVEATRIGAFSFLE
                     KPITLQKLLKAVEQGLARSIPSAAQSAPTPDIADSPDMATAGNGAPPFASAVSAADQL
                     PLSHQGFNLDRPLREARDGFEKAYFEFHLAREGGSMTRVAEKTGLERTHLYRKLRQLG
                     VDLGRNKRAG"
     gene            13669..13744
                     /locus_tag="PK518_08975"
     tRNA            13669..13744
                     /locus_tag="PK518_08975"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13702..13704,aa:Phe,seq:gaa)
BASE COUNT         2537 a         4475 c         4563 g         2218 t
ORIGIN      
        1 ccatcctggc gaacttcttc gtcaacgaca acttccgcct gatggacagc ggcgcgcaac
       61 tggagtttgt ctccgagcgc ctgcgtggcg aggtggcacg ctttgacatc accgacaagt
      121 cgggcaaggt catcgttgcc aaggacaagc gcgtcacggc gcgccatacg cgtgaactgg
      181 agcagtccgg cacgacccat atcagcgtgc ccgaggactt cctgatcggc cgcgtggtgg
      241 cgcgcaacat cgtcgatccc gacaccggcg agatcctggc caaggccaac gaagaactga
      301 ccgaggccct gctcaagaag ctgcgctcgg ccggcgtgca ggagctgcag gccatctaca
      361 ccaacgagct cgaccagggc gcctacatct cgcagacgct gcgcatcgat gaaaccgtgg
      421 acgaattcgc cgcgcgcgtg gccatctacc gcatgatgcg ccccggcgag ccgccgaccg
      481 aagacgccgt gcaggccctg ttccagcgcc tgttctacaa ccccgacacc tatgacctgt
      541 cgcgcgtggg gcgcatgaag ttcaacgcca aggtcggccg cgacgaggcc accggcccca
      601 tggtgctgtc caacgacgac atcctctccg tggtcaagat cctggtcgat ctgcgcaacg
      661 gcaagggcga ggtggacgac atcgatcacc tgggcaaccg ccgcgtgcgc tgcgtcggcg
      721 agctggccga gaaccagtac cgcaccggcc tggcacgtat cgaaaaggcc gtgaaggagc
      781 gtctgggcca ggccgagcaa gagccgctga tgccgcacga cctgatcaac agcaagccga
      841 tctcggccgc gttgaaggaa ttctttggcg catcacagct gtcgcagttc atggaccaga
      901 ccaacccgct ggccgaaatc acgcacaagc gccgcgtctc ggccctgggc ccgggcggtc
      961 tgacgcgcga gcgcgccggc ttcgaggtgc gcgacgtgca cgtcacgcac tacggccgtg
     1021 tctgccccat tgagacgccc gaaggcccga acatcggcct gatcaactcg ctggccctgt
     1081 acgcgcgcct gaacgagtac ggcttcatcg aaaccccgta ccgccgcgtg gtggatggca
     1141 aggtgacgat ggagatcgat tacctgtcgg ccatcgaaga gggcaagtac atcattgccc
     1201 aggccaacgc cacgctggac aaggacggca agctcaccgg cgacctggtg tcggcgcgtg
     1261 agaagggtga atccaccctg gtatcggccg agcgcgtgga gtacatggac gtgtcgccgg
     1321 cgcagatcgt ttcggtggcg gcatcgctgg tgcccttcct ggagcatgac gacgccaacc
     1381 gcgctctgat gggcgccaac atgtcgcgtc aggccgttcc cgtgctgcgc ccggagaagc
     1441 ccatggtggg cacgggtatc gagcgcgtgg ccgccgtcga ctcgggcacc gtggtcacgg
     1501 cgcgccgtgg cggtgtggtt gattacgtcg atgccacccg catcgtgatc cgcgtgaacg
     1561 actccgaggc cgtggccggt gaagtcggtg tggacatcta caacctgatc aagtaccagc
     1621 gttccaacca gaacaccaac atccaccagc gccccatcgt caagcgtggc gacaagctgg
     1681 ccaagggtga tgtgatcgcc gacggcgcct ccaccgacct gggcgagatc gccatcggcc
     1741 agaacatgct gatcgccttc atgccctgga acggctacaa cttcgaagac tcgatcctga
     1801 tcagcgagcg cgtggtggcc gaagaccgct acacctcgat ccacatcgag gagctggtgg
     1861 tgatggcgcg cgacaccaag ctgggtgccg aggaaatcac gcgcgacatt cccaacctgt
     1921 cggagcagca gctgaaccgc ctggacgagt cgggcatcat ctacgtcggc gccgaggtgc
     1981 agcccggcga cacgctggtc ggcaaggtca cgcccaaggg cgagaccacg ctgacgcccg
     2041 aggaaaagct gctgcgcgcc atcttcggcg agaaggccac cgacgtgaag gacacctcgc
     2101 tgcgcgtgga tcagggctcg cagggcacgg tgatcgacgt gcaggtgttc acccgcgaag
     2161 gcattcagcg cgacaagcgc gcacagcaga tcatcgacga tgaactgaag cgcttccgcc
     2221 tggacctgaa cgaccagctg cgcatcgtcg aggccgacgc cttcgatcgt atcgagaagc
     2281 tgctggtgga caaggtcgcc aacggtggcc cgcagaagct cgccaagggc acgaagatcg
     2341 acaaggccta tctggatggc gtggagaagt tccactggtt cgatatccgc cccgccgacg
     2401 aggccgtggc cgcacagctc gaatccatga agaacgccat ggagcagacg cgccacagct
     2461 tcgacctggc gtttgaagaa aagcgcaaga agctcacgca aggcgacgag ctgcccgcgg
     2521 gcgtgctgaa gatggtcaag gtgtacctgg ccgtcaagcg ccgcctgcag cccggcgaca
     2581 agatggccgg ccgccacggc aacaagggtg tggtgtcgaa gatcgtcccg gtcgaggaca
     2641 tgccctacat ggccgacggc acgcctgccg acatcgtgct gaacccgctg ggcgtgccat
     2701 cgcgcatgaa catcggtcag gtgctcgaag tccacctggg ctgggccggc aagggcatgg
     2761 gccagcgcat cggcaacatg ctgcaggaac aggccaaggc ggccgagctg cgcaagttct
     2821 tcgaggaaat ctacaactcg cgtggccgca aggaagaact gtcccagctg agcgacgacg
     2881 aggtcatggc catggcgcgc gagctgacca ccggcgtgcc ctttgcatcc cccgtgttcg
     2941 acggcgcctc cgaggccgag atcaaggaca tgctcaagat cgcctacccg gacgacatca
     3001 aggagcgcaa gggcctgacc gacacgcgca cccaggccta tctgtacgac ggccgcacgg
     3061 gtgagcgctt cgagcgcccg acgaccatcg gctacatgca ctacctgaag ctgcaccacc
     3121 tggtggacga caagatgcat gcccgctcca ccggcccgta ctcgctggtg acgcagcagc
     3181 cgctgggcgg caaggcccag tttggcggcc agcgcttcgg tgagatggaa gtgtgggcgc
     3241 tggaagccta cggcgccgcc tacgtgctgc aggaaatgct gaccgtgaaa tccgacgacg
     3301 tgcagggccg taccaaggtg tacgagtcca tcgtcaaggg cgaacacgcc atcgaggccg
     3361 gcatgccgga gtcgttcaat gtgctggtca aggaaatccg ttccctgggc ctggacatcg
     3421 agttggaacg ctcctgatcg gggccgatta aatagaaaaa gtgagctgct cgcgcttgtt
     3481 tatcaaaaga ttcaatgtca tttgataaca gattgactgt caatcaagcg cgcacagcta
     3541 cgatttttga tgcaagggaa agagtcatat gaaatcgcta ctcgacctgt tcaagcaatt
     3601 cacgccggat gagcactttg atgccatcaa gatcggcatg gcctcgcccg agaagatccg
     3661 ttcctggtct ttcggcgagg tgaagaagcc cgagaccatc aactaccgca ccttcaagcc
     3721 cgagcgcgac ggcttgttct gcgccaagat cttcgggccc atcaaggact atgagtgcct
     3781 gtgcggcaag tacaagcgcc tgaagcaccg cggcgtgatc tgcgaaaagt gcggcgttga
     3841 agtcacgcag accaaggtgc gccgcgagcg catgggccat atcgacctgg cggcgccctg
     3901 cgcgcatatc tggttcctga agagcctgcc gtcgcgtctg ggcctggtgc tggacatgac
     3961 gctgcgcgac atcgagcgcg tgctgtactt cgaagcctat gtggtcaccg accccggcat
     4021 gacgccgctg aagaagttca gcatcatgag cgaggacgac tacgacgcca agcgcaagga
     4081 atatggcgac gagttcatcg ccaagatggg cgccgagggc atcaaggagc tgctcgaggg
     4141 tatcgacctc gagatcgaga tcgagcgcct gcgcggcgac ctgacgggct ccgaggtcaa
     4201 ggtcaagaag aacgccaagc gcctgaaggt gctggaagcc ttcaagaagt ccggcatcaa
     4261 gcccgagtgg atggtgctcg aggtgctgcc cgtgctgccg ccggatctgc gtccgctggt
     4321 gccgctggac ggtggccgct ttgccacctc tgacctgaac gacctgtacc gccgcgtcat
     4381 caaccgcaac tcgcgcctgc gccgcctgct ggagctcaag gccccggaga tcatcgcgcg
     4441 caacgaaaag cgcatgctgc aggaggccgt ggacagcctg ttggacaacg gccgccgcgg
     4501 caaggccatg acgggcgcga acaagcgcgc cctcaagtcc ctggccgaca tgatcaaggg
     4561 caagagcggc cgcttccgcc agaacctctt gggcaagcgc gtggactact cgggccgttc
     4621 ggtgatcacc gtgggcccga cgctcaagct gcaccagtgc ggcctgccga agctgatggc
     4681 gctggaactc ttcaaaccct tcatcttctc gcgcctggag gccatgggca tcgccacgac
     4741 catcaaggcg gcgaagaagg aagtcgaagc cggcacgccg gtggtctggg acatcctgga
     4801 agaggtcatc aaggaacacc cggtcatgct caaccgtgcg cccacgctgc accgtctggg
     4861 catccaggcc ttcgagccca tcctgatcga aggcaaggcc atccagctgc acccgctggt
     4921 ctgcgcggcc ttcaacgccg acttcgacgg tgaccagatg gccgtgcacg tgccgctgtc
     4981 cgtcgaggcg cagctggagg cgcgcacgct gatgctggcg tccaacaacg tgctgttccc
     5041 ggcctcgggc gagccctcca tcgtgccctc gcaggacgtg gtgctgggcc tgtaccacgc
     5101 cacccgcgag aagatcaacg gcaagggcga gggcatggtc ttcaccgaca tcggtgaggt
     5161 gcagcgcgcg ctggacgcgg gtgaggttga gctggccacc aagatcagcg tgcgcctgac
     5221 cgagtggaac aagaacaagg acacgggcga gttcgagccc gagaccaagt tggtcgagac
     5281 caccgtcggc cgtgccctgc tgtcggagat cctgcccaag ggcctggcgt tcagcaacct
     5341 gaacaaggcg ctgaagaaga aggaaatctc caagctcatc aacgccagct tccgcaagtg
     5401 cggcctgaag gaaaccgtgg tgtttgccga caagctgctg caaaacggct tccgcctggc
     5461 cacgcgcgcc ggtatttcca tctgcgtgga cgacatgctg gtgccggcgc agaagaccga
     5521 catcatcggt cgcgccgaga aggaagtgaa ggagatcgag cagcagtatg tctccggcct
     5581 ggtcacggcc ggcgagcgct acaacaaggt ggtggacatc tggggcaagg ccggtgacga
     5641 catctccaag gtcatgatgg accacctgaa gaaggaaaag accatcgacc gccacggcaa
     5701 cgaggtcgat caagagtcct tcaactccat ctacatgatg gccgactcgg gcgcccgcgg
     5761 ctctgctgcg cagatccgtc agctcgccgg catgcgcggc ctgatggcca agcccgatgg
     5821 ctccatcatc gagacgccca tcacggccaa cttccgcgaa ggcctgaacg tgttgcagta
     5881 cttcatctcc acgcacggcg cccgcaaggg tctggccgac acggcgctga agacggccaa
     5941 ctcgggttac ctgacgcgcc gtctggtgga cgtgacgcag gacctggtgg tgaccgagga
     6001 cgactgcggc accagcaatg gctcgctgat gcgcgccatc gtcgagggcg gtgaagtcat
     6061 cgaatcgctg cgcgaacgaa tcctgggccg cgtcgtggcc gaggacgtgc tgcaccccga
     6121 gacgcgcgac gtgctggtgg ccgctggccg catgctggac gaagacacga tcgaggagat
     6181 cgagaccgtc ggcgtggatg aggtcaaggt gcgcacggcg ctgacctgcg caacccgcta
     6241 cggcctgtgc gccaagtgct atggccgcga cctgggccgc ggtggcctga tcaacctggg
     6301 cgaagccgtc ggtgtgatcg ccgcccagtc gattggcgag cccggcacgc agctgaccat
     6361 gcgtaccttc cacatcggtg gcgcggcctc gcgtgcggcg gtggcctcca gcgtcgaggc
     6421 caagagcaac ggcatcatcg gcttcaacgc cacgatgcgc tatgtgacca acaccaaggg
     6481 cgagctggtg gtgattgccc gttccggcga gatcgtcatc caggacgagc atggccgcga
     6541 gcgcgagcgc cacaaggtgc cctacggtgc gacgttgtcg gtcaaggccg accagcagat
     6601 caaggccggc accatcctgg ccaactggga tccgctgacc cgccccatca tcaccgagtt
     6661 cgccggcacg accaagttcg agaacatcga agaaggtctg acggtggcca agcaggtgga
     6721 cgatgtcacc ggcctgtcca cgctggtggt gatcgacccc aagcgccgtg gctccgccaa
     6781 ggtggtgcgc ccgctggtca agctgatcga cgccaacggc caggaggtga agatcccggg
     6841 caccgaccat gcggtggcca tcggcttcca gatcggcgcg ctgatccagg tgcgcgacgg
     6901 ccaggacgtg ggcccaggcg aggtgctggc gcgcattccg gtcgaaggcc agaagacgcg
     6961 cgacatcacc ggcggtctgc cgcgcgtggc cgagctgttc gaggcccgca gccccaagga
     7021 caagggcatg ctggccgaga tgaccggcac catctccttc ggcaaggaga ccaagggcaa
     7081 ggtgcgcctg cagatcaccg accccgatgg ccatgtctgg gacgaactgg tgcccaagga
     7141 gaagaacgtg ctggtgcacg agggccaggt ggtgaacaag ggcgagctga tcgtcgatgg
     7201 ttcggccgat ccgcaggaca tcctgcgcct gctgggcatc gaggagctct cgcgctacat
     7261 cgttgatgag gtgcaggacg tgtaccgcct gcagggcgtg aagatcaacg acaagcacat
     7321 cgaggtcatc gtgcgccaga tgctgcgccg cgtcgtggtc gagaacccgg gcgagaccag
     7381 ctacatccag ggcgagcagg tcgagcgctc ggagatcctc aacaccaacg agcaggcgca
     7441 aaaggacggc aagatcccgg ccacctactc caacgtgctg ctgggcatca ccaaggcctc
     7501 gctgtcgacc gactccttca tctccgcggc ctcgttccag gagacgacgc gcgtgctcac
     7561 cgaggctgcc atcatgggca agcgcgacga gctgcgcggc ctgaaggaaa acgtcatcgt
     7621 cggtcgcctg atccccgccg gtaccggtct ggcctaccac caggcgcgca aggtcaagga
     7681 cgccatggat gaggccgagc gccgcgccat cgccgaggcc gaagcggccg agctggccag
     7741 cgccaatgcc gaaggcgctg ccgctgagct cgacggcagt gccgacctgg cggactaagc
     7801 cctggtacgg caacccggaa atatcgaaac atgaaagcgc gccttcgcac ccacggcggc
     7861 gttgcaaatc ctcgcgatag ctacggctat cgctgcggtt tgcgccttgc cctgggcgcg
     7921 aatccatcac tttcattgtg ttcctctact tccaggttgc cgcactagcc tcgccgctac
     7981 catgctgctc ccggcttgcc aattggcggg ccgggagttt cttttttggg gttgtttcgt
     8041 ggcgccgcag cttatgaaca tgtggatttc ctgggtgttt ctggcattgc tgctgttttg
     8101 ggccgtgggc gcctacaacc gcctgattcg cctgcgctcg gccgcgctgc aggcctttgg
     8161 cgcactggat gcgcactggc agcgctggat agcgttgttg gcgcaatatg cggcttcacg
     8221 tatcgaagcc acagaggacg atgcatcggc gggtggcgcc gccgacaccc aggccgccct
     8281 ggacgcagcg gcggcgcaat ttgccgcctc tctggcagtg gcccgggccc ggccgctgga
     8341 tggcgacgcc gctgcaaccc tgtcggcagc ggcccaggtg ttggacacgg cctggcagtc
     8401 cctggcccat gaggcggccc gggcgggcga gggcgtggcg ccaccggccc tggcgccatg
     8461 gatacaccag cgcgaacagc tggcgctgca cgcgggcgag gccaggcggc tgttcaatga
     8521 agcggttggc caatacaacc acgctatcgg gcagtttccg gccaacctgc tggcctggct
     8581 gtttggcatg aagaaggggc gcgcgctgtg accaccacca agccatccgc gccaggcggc
     8641 gccgcatcgc cgcccttgag ccgcttgctg gacgccaccg ccaccgccct gcaggcgata
     8701 cgtgcgggcc agtcgggcac ggccgcgctc gatgccagcg atgcagcgct gcgcccggcc
     8761 gtgcaggcgc tgttgttcca ggtgctgcgc aacctcgggc gcgcccaggc cttgcgccgg
     8821 cagctggcac cacgcaaccc gccggcgcgg gtggatgcgc tgctgtgcac ggccctagcg
     8881 ctggcctggg acactgaggc cgcgccctac gagccgttca ccctggtgaa ccaggcggtg
     8941 cacgccgcca agcacagcgc ggccacgcgc gcccaggccg gctttgtcaa cgcctgcctg
     9001 cggcgtttcc tgcgcgagcg cgaggcactg gtggtggcca cccagggtga cgaggtagcg
     9061 gtctggaacc acccggcatg gtggatcaag cgcctgcgcc aagaccaccc ggagcgttgg
     9121 caacagatcc tgcaggccag caacctgcct gcacccatgg tgctcagggt ttcaaagaaa
     9181 aaaagtagtc cagcgcttta ccagcaaacg ctaacagcta tcaaaattga atcaacattg
     9241 gtgggtgata gcggcctggc actggcccgt ccggtgccgg tgggccagct acccggcttt
     9301 gccgatggcg tggtttcggt gcaggacggg gccgcgcaga tggcggcgcc gctgctgctg
     9361 gccgggctgg atgccagcca gccgctgcgc ctgctggacg cctgcgccgc ccctggcggc
     9421 aagaccgcgc acctgctgga atgggctgcc gcgctgcgcg cgccatggca ggtcaccgcg
     9481 ctggaggtgg acgcacagcg cgccgagcgc atccacgaca acctgcgccg cctgggcctg
     9541 agcgcgcagg tcgtggtggc cgacgccgca cgccccgcag actggtggca acaggccagc
     9601 ggcggcgcct tgttcgacgc catcttgctc gacgcgccct gcacggcatc gggcatcgtg
     9661 cgccgccatc ccgacgtgcg ctggctgcgc cgcgagagcg acgtggcgca gctggcgggc
     9721 cagcaggcgc gcctgctggc ggcgctgtgg ccgctgctgc gccccggtgg gcgcatgctg
     9781 tactgcacct gctcggtgtt ccgggccgag ggcgacgagc agatcacggc gtttcttgcg
     9841 cacaacaccg atgcgctttt gctgccatct ccgggccatt tattcccggg caacccggat
     9901 atgcgccacg cgctcccgga caatgggccg ggtgaacacg acggtttctt ctacgccttg
     9961 ctacaaaaaa agacctgaat ccggtgcgcg cgcctgggtg cgctggtgcg cagcgctggc
    10021 ctgcctgctg gcgctgtgct ggtacgcgcc ggccgcgcag gcgcagcagc atggcgaggt
    10081 cagcgatctg cagctcgagc gcggcgccga cggcctgtac ctctcggccg cgctgcggct
    10141 ggagctgccc acgatcgtgc aagatgccct gtacaaaggc atctccatgc atttcattgc
    10201 cgacgccgag gtgctgcgcc cgcgttggta ctggacggac aaggtcgtgg cgcatgccac
    10261 gcgctatgtg cgtctgagct accagccgct cacgcgccgc tggcgcctgg cacagtctgc
    10321 agcgccattt gcggccactg gcctgggggt gtcgctggat cagagctttg atgacctgcc
    10381 cgaggtgctg gccgccctgg agcgcatcgc gcgctggaag atcgcggaag agggcgtcat
    10441 cgacgagggc cagtcctatt tggtgaactt ccagttccgc ctcgacatgt cgcaactgcc
    10501 ccagccgctg cagataggcg ccgtgggcgg ctccagctgg aacattgccc tcgcgcgcgg
    10561 cgcgcgcctg agcgcccagg aggccacgcg gtgaagatgg gcgagcagcg ccccgcgggc
    10621 tcggcacgcc gggcggcgaa ccgggcgcgg gccctgcgct gggcggtggg cctgggcgcc
    10681 ggcgtgatgg gtgccatagg cctggtgctg ctgtttctgc tcaccctggc caccaacaat
    10741 cgcgcgctct acgagcgcaa ctttgcctgg ctgctgggcg tgaacgtgct tgtcgccgtg
    10801 gcgttgttgg cggtgctggt ctggggcgcc gtgcgcctgg gcgtgcgcgt gcggcgcggg
    10861 cgattcggca gccatctgct gatcaagctg gcggccatat tcggcctggt gggggtggtg
    10921 ccggggctgc tgatctatgt cgtgtcctac cagttcgtct cgcgctccat cgagagctgg
    10981 ttcgatgtca aggtggaggg tgcgttgacg gccggggtga gcctggcgcg cgtcacgctg
    11041 gacaccattg ccagcgacct ggcgaccaag acccgcggcg ccagcagcca gctggcgcag
    11101 gtgcccgatg ccgcggccgg cgtggtgctt gagcgcatgc gcgatcagct cggggcttcg
    11161 gacatggtgt tgtggaatgc cgcgggccag cccgtggccg gggtcgggca atcgcgctat
    11221 tcgctcaacc ccgatcgccc cagcgcgcag caactgcgcg ccgcgcgcca ggagcgcatc
    11281 agcttccaga tcgaggggct ggacgacatc aacgatccca tggctgcgga gaacgcacgc
    11341 gtgcgcgtgc tggtgctggt agtccatccg ggcgtggggc tgttggccga gccgcgctac
    11401 ctacaggcca ccctgcctct gccccaggcc ctggtggcca acgccatcgc cgtgcaggag
    11461 gccaaccgcg aataccagga gcgcgcattg gcccgtggtg gcctgcggcg catgtacata
    11521 ggcacgctga ccctggcgct gtttctggcg gtgttcggcg cggtgctgtt ggcggtggtg
    11581 ctgggcaacc agctggcacg tcccctgatt gtgctggcag acggtgtgcg cgaggtggcg
    11641 gccggcaacc tggcgcccaa ggccgcgctg caaggcaagg atgagctcgg cggcctgacg
    11701 cgatcctttg ccctgatgac gcaacagctg gccgatgcgc gctcggccgt ggagcgcagc
    11761 atggagcagc tggatgcggc gcgcggcaac ctgcagacca tcctagacaa cctcacggcg
    11821 ggcgtcatcg tgctcgatca gcagggcgtg atccagtcct ccaacccggg cgccacgcgc
    11881 attctgcgtg caccactggc cgctcacgag ggcaaacccc tggccgccgt gcccggtctg
    11941 gccgaatttg cggccacggt gcagcagcat ttcgacgctt tctcgctgga ccatggccac
    12001 catagtctgg atcactggca gcacccgttt gagctgcatg gctctggcgg cggcatgcac
    12061 gcccagggca ccagcctggt cgcacgtggc gccgagctgc ccaaccacgc gcgtctgctg
    12121 gtgtttgacg acatctccga aatcgtctcg gctcagcgcg cccaggcctg gggcgaagtg
    12181 gcgcgccgcc tggcgcatga aatcaagaac ccgctcaccc ccatacagct gtcggccgag
    12241 cgcctggagc tcaagctcac gggcaagctg cccgacgccg agcaggccgt gctcatcaag
    12301 tcggtcaaga ccatcgtcga tcaggtcgat gccatgaagc gcctggtcaa cgagtttcgc
    12361 gactatgcgc gcctgcccgc cgccgtgctg cagccgctgg acctgaatgc attgattgcc
    12421 gatgtgctgc acctgtatgg cgccgagacc gcgcaggtgc cggtgcaggc cgagctggac
    12481 gtgcgttgtc cgcgcatcct gggcgacgcc cagcaactgc gccaggtggt gcacaacctg
    12541 ctgcagaacg cgcaagatgc caccttgcaa gcggccgagc agcagaagct gccgcccgca
    12601 cctgtggtca tcgccacgcg ctggagtgag tcggcgcagc gcgtgcggct cacggtgtcc
    12661 gattgcgggc caggctttcc ggcgtatatc ctgcaacgcg cctttgagcc ctatgtcacc
    12721 accaaggcgc gcggcaccgg tttgggcctg gcggtggtca agaaaattgc cgatgagcat
    12781 ggtgcgcgca tcgacctggc caaccgtatc gagggcgagg aaatgagggg cgcgcaagta
    12841 tcgctatcat tcgctcctga agcggcggtg gcgctctaac aacatcgcgg taccggcagt
    12901 aaggatgcat ttacacacat ggcaaacata ctggtagttg acgacgagct ggggatccgg
    12961 gatctgttgt cggaaatcct gaacgatgag gggcatagcg tggatctggc cgagaacgcc
    13021 acgcaggccc gcagcgcgcg gcaggcgaac agctatgacc tggtgctgct cgacatctgg
    13081 atgcccgaca ccgatggcgt ctcgctgctc aaggaatggg ccatggccgg cacgctgacc
    13141 atgcccgtga tcatgatgag cggccacgcc acgatagaca cggccgtcga ggccacccgc
    13201 atcggggcgt tctctttcct ggaaaagccc atcaccctgc aaaaattgct caaggccgtg
    13261 gagcaaggcc tggcacgcag cattcccagt gcagcgcaga gtgcaccaac ccctgacatc
    13321 gccgactcgc cggacatggc aactgccggc aacggtgcac caccttttgc gagcgccgta
    13381 tcggcggccg accagctgcc cttgtcgcac cagggcttca atctggatcg cccgctgcgc
    13441 gaggcgcgcg acggttttga gaaagcctac tttgagttcc acctggcccg cgagggcggc
    13501 tccatgacgc gcgtggccga aaagaccggc ctggaacgca cccacctcta ccgcaagctg
    13561 cgccagctgg gtgtcgacct gggccgcaac aagcgcgccg gctaagggtc gcaaattttc
    13621 aaggtttcaa attggatgcc atttttttgc gctagaatcc gtggctcagg cccggtagct
    13681 cagtcggtag agcagaggat tgaaaatcct tgtgtcggcg gttcgattcc gtcccaggcc
    13741 accaaatcgc aacgcccaac cgttctcggt tgggcgtttt tttgtggatc gga
//
LOCUS       DAOMTQ010000078        13791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_747_length_13791_cov_26.429747, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000078 DAOMTQ010000000
VERSION     DAOMTQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_13791_cov_26.429747"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1261
                     /locus_tag="PK518_08980"
     CDS             299..1261
                     /locus_tag="PK518_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRM49089.1"
                     /translation="MKRILLSVVCLAAAASQAQVKWDLPTGYAANTFQTQNNQQFAKE
                     VDELTGGKLKITLHPGGSLYKANEIKRAVQTGQVPAAEFILSGAANENPLYGVDSVPF
                     LASSYADAWRLYQAAKPAQEKLLASQGMQLLYSVPWPGQSLYSNKPVQSPADLVGTKM
                     RAYNPTSTRIAQLLKAQPVTIQLSELGQALATNTVNNFLTSSASGVESKLYEQIKYFY
                     PVNAWLPRNATVVNKKAFDSLDKGLQDAVLKAAATAEKRGWESSERLDKEFLKELAAK
                     GMTVSEPSDALKKEFARIGTVMTEEWVKAAGAEGKAIVDAYNKR"
     gene            1339..1848
                     /locus_tag="PK518_08985"
     CDS             1339..1848
                     /locus_tag="PK518_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRM49090.1"
                     /translation="MDQLFRLLYRLLIALACLSMVAAFVIVMLGILARQVTFIDIQGL
                     DAYAGYAIAATLFFALPTALMHGDHIRVTLLLDRMGSRTRAAFEWAMLIAGLGLALYL
                     AWFAARAVWLSYITHDVSPAADATPLWIPQLCMAIGCAAFALAFLDAIVARWRGREWF
                     IAGPGSVAE"
     gene            1860..3152
                     /locus_tag="PK518_08990"
     CDS             1860..3152
                     /locus_tag="PK518_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRM49091.1"
                     /translation="MDMMAALVLIIALFAVLGAGMWIGLGLLAVGLIGMGLFTSRPVG
                     DSMMLTIWGASSAWTLTALPLFLWMGEILFRSKLSDSMFRGLAPWMDKLPGRLLHTNV
                     VGCTIFAAISGSSAATCATIGKMTLPELKKRGYPDAMAVGTLAGSATLGLLIPPSIIM
                     IVYGVAANVSIAKLFLAGVFPGLLLAGLFMAYIATWALFNRDKVPASDMSMGFMQKIY
                     ESRFLIPAVILIGLVLGGIYSGVATATEAAALGVAGALGLAAIEGSLTWQKFTEGLVA
                     ACRVYCMIGLILAGAAFLTLAMGFIGLPRHLAEWIGSLHLSPFALLMMLTVFFIVLGC
                     FLDGISMVVLTIAVLLPTVEAAGYDLIWFGIFIVLVVEMAQITPPVGLNLFVIQGLTK
                     RDIGWIARVAAPMFFVMLAAVIMLYVWPQIVLWLPSHM"
     gene            3164..3922
                     /locus_tag="PK518_08995"
     CDS             3164..3922
                     /locus_tag="PK518_08995"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRM49092.1"
                     /translation="MSVIDLNSDLGESLGAWSMGDDAAMLDIVSSANVACGFHAGDPA
                     GILATLRAAKARGVAVGAHVAYPDLIGFGRRNMDVASPDLVADVIYQIGALQGLAQAA
                     GTRIAYVKPHGALYNTIAHDERQARDVIAAIRAVDAGLALVVLAASPLVQWARDAGLR
                     VIAEAFADRGYTPQGTLVSRRDKGAVLHDSALVARRMLRLVKDGVVEAIDGSLVHLGA
                     QSICVHGDSPGAVEMARAVRKALEADGVAVRPFV"
     gene            3948..4760
                     /locus_tag="PK518_09000"
     CDS             3948..4760
                     /locus_tag="PK518_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HRM49093.1"
                     /translation="MTNNPLQQAHTPAQAAARQARAAYRAGLKQPTAGVAPGMTQANM
                     IALPRDWAWDFLLYAQRNPKPCPVLDVIEAGAHHTVLAEGADIRTDIPLYRVWRHGKL
                     AEEVQDATALWAEHPDLVTFLIGCSFTFETPLQEAGIEVRHIADGGNVPMYRTSRQCR
                     PAGRLHGEMVVSMRPIPAHRVADAVSISARFPAVHGAPVHVGDPAALGIADLARPDFG
                     DPVRIEPGEIPVFWACGVTPQAAVMASGVPFAVTHAPGYMFITDVPDSSYHV"
     gene            4762..6366
                     /gene="pxpB"
                     /locus_tag="PK518_09005"
     CDS             4762..6366
                     /gene="pxpB"
                     /locus_tag="PK518_09005"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRM49094.1"
                     /translation="MRFLPVSLNALLVELADLDQTLALLASLQREPLPGVQELVPAAR
                     TILVQFRPSATSVAALVQAIAARDLSQQVRRDATLVEIPVHYDGEDLAEVAQILGITP
                     EELVRRHTGSDYSVAFVGFAPGFAYLSGGHESLDVPRRATPRTRIPAGAVALAGRFSG
                     VYPQASPGGWQILGTTPLAMWDIEREQPALLQPGFRVRFVDIATLSVAVRAQLERAAD
                     QKHLKPAAPAPARGPALEVRSPGLLTLLQDMGRPGLAGQGVSASGALDQGACRTANRL
                     VGNPPDMACLETVNGGLRLMSHGDTVVAVTGADAPLTLTSAGGLRTSVPRYQPLALAH
                     GDLLAIGEPVAGTRCYVAARGGYDVAPILGSLSTDTLAHVGPPALAAGDRLALRPVAR
                     GSIVGGAELPPDDLPGPDGEVVLDVIMGPRTDWFTPQAVQLLASQRWRVTPQSNRVGL
                     RLAGEQPLTRSITQELPSEGTPTGAIQVPASGQPVLFLADHPLTGGYPVIACIAPHHL
                     DRAGQIPVNAWVRFNPIRPFEAINRP"
     gene            6372..6509
                     /locus_tag="PK518_09010"
     CDS             6372..6509
                     /locus_tag="PK518_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49095.1"
                     /translation="MKKVLIANRGENDRAAGVSSHCGACAAGDGVLAIQHLLKAKGLK
                     S"
     gene            6506..8233
                     /locus_tag="PK518_09015"
     CDS             6506..8233
                     /locus_tag="PK518_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRM49096.1"
                     /translation="MKKVLIANRGEIAVRVVRACRDYGVQSVAVYANADIDALHARMA
                     DEAWGLDGDRPADTYLHIEKLLDIARKSGADAVHPGYGFLSESAAFAQAVLDAGLAWI
                     GPTPQAIARLGDKLEARKIALKVGAPLVQGTSEPAKDAAEVLAFAEQHGLPIIIKAAF
                     GGGGRGMKIAWRMDEVAELYASAVREATAAFGRGECFVEQFLDKPRHIEAQVMGDKHG
                     KVVVLGTRDCSLQRRNQKLVEEAPAPFLTDAQRQRIHQAAHDICAEVGYESAGTVEFL
                     LSGSGALSFLEVNTRLQVEHPVTEQTTGLDLVLEQLRVADGLPLSITETPAPLGHAFE
                     FRINAEDVGRGFLPTPGPVQRFDAPSGPGVRVDSGVQAGSIVPGTFDSLMAKLIVTGS
                     TREQALARARRALAEFQIDGVASVLPFHRAVMEQADFIAADGFKVHTRWIETAFANDL
                     AAAVRAAPQADASLVRCAIEIDGRRLQLGLPAVLLQGLAAAGGASATAAVAQESVDEA
                     AVAAPIAGNLHAWKVGEGDAVQAGDVIAVMEAMKMEMQVTAHRSGRITLQAAAGSYQA
                     AGSCIARIT"
     gene            complement(8298..10070)
                     /gene="msbA"
                     /locus_tag="PK518_09020"
     CDS             complement(8298..10070)
                     /gene="msbA"
                     /locus_tag="PK518_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRM49097.1"
                     /translation="MRDLNHSAPKPPQPPTPSLLARLRRLHAYFGYQRLAWAMALLAT
                     VVAAITEPLIPALLKPLLDQGFTEGSLNLWMVPTAIIGVFLVRGLAQFTGQYALSRIA
                     NEGMLRLRTALFERLLAADMALFRQQSASTLSNTVVYEVQTGFTSLVQALIGVSRDGF
                     TLVALLGYLIYLNWQLTLIVAVMVPGVGWIMKALSRRLYKITKSSQQATDDLAYVVEE
                     NVLAHRMVRLHGAQAGQAARFNNLSQRLRHLAIKSTIASAAMTPTTQLLAAGALSAVI
                     CIALWQSHGHGAKDVSVGGFASFIAAMLMLIAPIRRMADVANPITRGVAALERGLLLL
                     SDAPAETGGTHAVQRAQGAISLRDVNVSFDSEHAPALSGVSLDIRPGEIVALVGPSGA
                     GKTTLVNLLPRFVAASGGQVLIDGVAAQDWRLESLRAQFAMVSQDVVMFNDSIAANVA
                     LGAAIDEERVQHCLAAANLAEHVAALPQGVHTIVGHNATQLSGGQRQRLAIARALYKD
                     APILILDEATSALDTESERLVQDALQRLMRGRTTLVIAHRLSTIEHADRVVVMERGRI
                     AEQGTHAELVAAGGLFARLQAHTS"
     gene            10190..11509
                     /gene="tolB"
                     /locus_tag="PK518_09025"
     CDS             10190..11509
                     /gene="tolB"
                     /locus_tag="PK518_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRM49098.1"
                     /translation="MTIERTFPSSPLRAAMPHISRRHAVAALAAASSLPALAQFRVEI
                     TGVGLTQLPIAIAPLRGEAQSPQKISAIVQADLERSGRFVGVDASGVPLDETSRPDLS
                     QWRQRKADALNTGSVTRLADGRFDVRFRLWDVVKGQDLGGQSFVVTQADLRLVAHRIA
                     DFIYEKLTGERGVFSTRIAYVTKVGARYSLWVADADGENAQSALSSPEPIISPAWSPT
                     GTQLAYVSFESRKPVVYVHDVATGKRRLIANFRGSNSAPAWSPDGRTLAVTLSRDGGS
                     QLYTIDAGGGEPRRLMQSAGIDTEPAFSSDGRSIYFVSDRGGSPQIYRVGSGGGNAER
                     VTFNGSYNISPAISPDGQWLAYISRIGGAFKLQVMDLKSGTVSSLTDTSADESPSFAP
                     NSRLILYGTQQQGREALMTTTLDGKIKARLAGQGGDIREPDWGPFQK"
     gene            11554..12099
                     /gene="pal"
                     /locus_tag="PK518_09030"
     CDS             11554..12099
                     /gene="pal"
                     /locus_tag="PK518_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRM49099.1"
                     /translation="MKSTPFKRLSIAFTIAALMAGCSSGVKLDDVPVEDKGATSTTGG
                     GAGAGGAGQSGVAPVDLSQSSRDVGGPVGVARIIYFDFDSYTVKPEFQSLLEAHARYL
                     KASSARKVMLEGHTDDRGGREYNLALGQKRAEAVRRSLGLLGVSDAQVEAVSYGKEKP
                     AVQGNNESAFAQNRRVELSYR"
     gene            12111..12887
                     /gene="ybgF"
                     /locus_tag="PK518_09035"
     CDS             12111..12887
                     /gene="ybgF"
                     /locus_tag="PK518_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRM49100.1"
                     /translation="MSFPQRSRALTVAVFAAVATWAVPSHALFEDDEARRAILELRQR
                     FDALQQSNQRSGERSGEEVSQLRRSLLDLQTQIEALRTEQASLRGQNEQLQREVAELQ
                     RRQKDIAQGVDERLRKFEPLAVTVDGREFRADPAEKREFEAALAVFRSGKFPEAATAF
                     GSFMRQYPQSGYLPSARFWLGNAQYATRDYKEAINNFKSLLAAAPDHARAPEAALSIA
                     NCQIELKDTRAARKTLEDLMRVYPQSEAAAAAKERLARLK"
     gene            12884..13675
                     /locus_tag="PK518_09040"
     CDS             12884..13675
                     /locus_tag="PK518_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRM49101.1"
                     /translation="MSVPAQESADLQRRFGGLQRLYGVAGAQRIRAAHVAVIGIGGVG
                     SWAAEALARSGVGRLTLIDMDHVAESNVNRQIHTLSTTLGQAKVRAMAERIALINPEC
                     RVDCIEEFVDPANWPTIVPAPVDAVIDACDQVRAKAAMAAWAMQTRTAFITVGAAGGK
                     RLAHKVDVDDLAHTTHDPLLAQLRYRLRREHGAARDGKRIGVACVFSREAVAPPHASC
                     AIDEGDGSLNCHGYGSSVAVTATFGHCAAGWLLDKLSADVPKNTL"
     gene            13710..13785
                     /locus_tag="PK518_09045"
     tRNA            13710..13785
                     /locus_tag="PK518_09045"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13743..13745,aa:Lys,seq:ttt)
BASE COUNT         2205 a         4617 c         4596 g         2373 t
ORIGIN      
        1 gccagcaccc gcatgcccgt ttgccgcatg gcggcagcgc tgcgggggtg atctcagggt
       61 ttttcaggat gcaaattgcc actcatttga taaatcattg ttgaacaatt taagttgaat
      121 ggttctgcga tcctgcggtg cttgtgggct gtaggacagg ttccgcagcg tcggggcgct
      181 gcttgctttt tgcccgcgga agtttgtaaa tgcgatctgc gcatgggcgc cgcagcccag
      241 cctcgtggga cttgccttca cttgtttgtt ttcaactgtc aatccaagga gtcacctcat
      301 gaaacgcatt cttctgtcgg ttgtctgcct ggcggcggcg gcttcccagg cccaggtgaa
      361 gtgggatctg cccacgggct acgcggccaa caccttccag acgcagaaca accagcagtt
      421 cgccaaggag gtggacgagc tcaccggcgg caagctcaag atcacgctgc acccgggcgg
      481 ctcgctgtac aaggccaatg agatcaagcg cgcggtgcag accggccagg tgccggcggc
      541 cgagttcatc ctctccggtg cagcgaatga gaaccccttg tacggcgttg actccgtgcc
      601 cttcctggcc agctcgtacg ccgatgcctg gcgcctgtac caggcggcca agccggcgca
      661 ggagaagctg ctggcctcgc agggcatgca gctgctctac agcgtgccat ggccgggcca
      721 gtcgctgtac tccaacaagc cggtgcagtc gcctgccgac ctggtgggca ccaagatgcg
      781 cgcctacaac ccgacctcca cacgcatcgc ccagctgctc aaggcgcagc cggtcaccat
      841 ccagctgtcc gagctcgggc aggcgctggc cacgaacacg gtgaacaact tccttacctc
      901 cagcgccagc ggcgtggaga gcaagctgta tgagcagatc aagtacttct acccggtgaa
      961 cgcctggctg ccgcgcaatg ccacggtggt caacaagaag gccttcgaca gcctggacaa
     1021 gggcctgcag gacgccgtgc tgaaggctgc cgccacggcc gagaagcgcg gctgggagag
     1081 cagcgagcgt ctggacaagg agttcctgaa ggagctggcc gccaagggca tgaccgtctc
     1141 cgagccgtcg gacgcgctga agaaggagtt cgcccgcatc ggcaccgtca tgaccgagga
     1201 atgggtgaag gcggcgggcg ccgagggcaa ggccatcgtc gacgcgtaca acaagcgctg
     1261 acctgtctca tcgggacgcg ggccgcatag gcactgcgtc cctttccatc cactttcaga
     1321 gcccgcccgg agcccgccat ggatcagctc tttcgactct tgtaccgcct gctcatagcg
     1381 ctcgcctgcc tttccatggt ggctgcgttc gtgatcgtca tgctcggcat cctggcgcgc
     1441 caggtgacgt tcatcgacat ccaggggctg gatgcctatg ccggctacgc cattgccgcc
     1501 acgctgttct ttgccttgcc cacggcgctg atgcatggcg accatatccg cgtcacgctg
     1561 ctgcttgacc gtatgggcag ccgtacccgt gcggcttttg agtgggccat gctgattgcg
     1621 ggcctgggcc tggcgctcta tctggcctgg tttgccgcgc gcgccgtgtg gctgtcctac
     1681 atcacgcacg acgtctcacc cgcggccgac gccacgccgc tgtggattcc gcagctgtgc
     1741 atggcgattg gctgtgcggc ctttgcgctg gcgtttctgg atgccatcgt ggcgcgctgg
     1801 cgcgggcgtg aatggttcat tgccggcccg ggctcggtgg ccgaataaag gagaacaaca
     1861 tggacatgat ggctgcactg gtgctgatca ttgcgctgtt cgcggtgctc ggcgcgggca
     1921 tgtggatcgg cctgggcctg ctggccgtgg gactcatcgg catggggctg ttcaccagcc
     1981 gcccggtggg cgacagcatg atgctgacga tctggggcgc cagctcggcc tggacgctga
     2041 cggcgctgcc gctgtttctg tggatgggcg agattctgtt tcgcagcaag ctatcggaca
     2101 gcatgttccg cggcctggcg ccgtggatgg acaaactgcc agggcgcctg ctgcacacca
     2161 acgtggtcgg ctgcaccatc tttgccgcca tctccggctc ttcggccgcc acctgcgcca
     2221 ccatcggcaa gatgaccctg cccgagctga aaaagcgcgg ctaccccgac gccatggccg
     2281 tgggcacgct ggcgggctcg gccacgctgg ggctgctgat tccgccctcc atcatcatga
     2341 tcgtctatgg cgtggcggcg aacgtgtcga ttgccaagct gttcctggcc ggcgtgttcc
     2401 cggggctgct gctggccggg ctgttcatgg cctatatcgc tacctgggcg ctgttcaacc
     2461 gggacaaggt gccggcgtcg gacatgtcca tgggcttcat gcagaagatc tacgaatccc
     2521 gctttctcat accggcggtg atcctgatcg gcctggtgct cggcggcatc tatagcggcg
     2581 tggcaaccgc caccgaggcc gctgccctgg gcgtggccgg cgcgctgggc ctggcagcca
     2641 ttgaggggtc gctgacctgg cagaagttca ccgagggcct ggtcgccgcc tgccgcgtgt
     2701 actgcatgat cggcctgatc ctggccgggg cggccttcct cacgctggcc atgggcttca
     2761 tcggcctgcc gcgccacctg gccgaatgga taggttcgct gcacctgtcg ccgtttgccc
     2821 tcttgatgat gctgacggtg ttcttcatcg tgctgggctg ctttctggac ggcatctcca
     2881 tggtggtgct gacgattgcc gtgctgctgc ccacggtcga ggcggcgggc tatgacctga
     2941 tctggttcgg catcttcatc gtgctggtgg tggagatggc gcagatcacg ccgcccgtgg
     3001 ggctgaacct gttcgtcatc cagggcctga ccaagcgcga cataggctgg atcgcccgcg
     3061 tggccgcgcc catgttcttc gtcatgctgg ccgccgtgat catgctctat gtctggccgc
     3121 agatcgtgct ctggctgcct tcgcacatgt aagggagtgc gccatgtccg tgatcgacct
     3181 gaacagcgac ctgggcgaga gcctgggcgc ctggagcatg ggcgacgacg ccgccatgct
     3241 cgacatcgtc agcagcgcca acgtggcctg cggctttcat gcgggcgacc cggccggcat
     3301 cctggccacg ctccgggccg caaaggcgcg cggcgtggcc gtgggcgcgc atgtggccta
     3361 tccggatctg atcggctttg gccggcgcaa catggatgtg gccagccccg acctggtggc
     3421 cgacgtgatc taccagatcg gcgccctgca gggcctggcg caggcggccg gcacgcgcat
     3481 cgcctacgtc aagccccatg gcgcgctgta caacaccatt gcgcacgacg agcgccaggc
     3541 gcgcgacgtg attgccgcca tccgcgccgt cgatgccggc ctggcactgg tggtgctggc
     3601 ggcctcgccg ctggtgcaat gggcgcggga cgccggcctg cgcgtgatcg ccgaggcctt
     3661 tgccgatcgt ggctacacgc cccagggcac gctggtgtcg cgccgcgaca agggcgccgt
     3721 gctgcacgac agcgccctgg tggcccggcg catgctgcgc ctggtgaaag acggcgtggt
     3781 cgaggccatc gatggcagcc tggtgcacct gggcgcccag tccatctgcg tgcacggcga
     3841 cagccctggc gccgtggaga tggcgcgcgc cgtgcgcaag gcgctggagg cggacggcgt
     3901 tgccgtgcgg ccatttgttt gacggctgat tgacgggagc cagcaccatg acgaacaacc
     3961 cattgcaaca ggcccacacc cctgctcagg ccgccgctcg ccaggcgcgc gccgcctacc
     4021 gcgccggcct gaagcagccc acggcaggcg tggcgcccgg catgacccag gccaacatga
     4081 ttgcgctgcc gcgcgactgg gcctgggact ttctgctcta cgcccagcgt aaccccaagc
     4141 cctgcccggt gctggacgtg atcgaggccg gtgcgcacca cacggtgctg gccgaaggcg
     4201 ccgacatccg caccgacatt ccgctgtacc gcgtctggcg ccatggcaag ctggccgagg
     4261 aggtgcagga cgccacggcc ctgtgggccg agcacccgga cctggtcacc tttctgatcg
     4321 gctgcagctt cacgtttgaa accccgctgc aggaggccgg catcgaggtg cgccacattg
     4381 ccgacggcgg caacgtgccc atgtaccgca ccagtcgcca atgccgcccg gcagggcgcc
     4441 tgcatggcga gatggtggtg tccatgcggc ccattcccgc gcaccgcgtg gccgatgcgg
     4501 tcagcatctc ggcgcgcttt cccgccgtgc atggcgcacc cgtgcatgtg ggcgacccgg
     4561 ccgcgctggg cattgccgat ctggccaggc ccgactttgg cgacccggtg cgtatcgagc
     4621 ccggcgagat ccccgtgttc tgggcctgcg gcgtcacgcc ccaggcggcg gtgatggcct
     4681 cgggcgtgcc gttcgcggtc acgcatgcgc cgggctatat gtttatcact gatgtgcccg
     4741 acagcagcta ccacgtctga tatgcgcttc ctgcccgtct cattgaacgc gctgctggtg
     4801 gagctggccg atctggatca gaccctggcg ctgctggctt cgctgcagcg cgagcccctt
     4861 ccgggcgtgc aggagttggt gcctgcggcg cgcaccatcc tcgtgcagtt ccgcccttcg
     4921 gccaccagcg tggccgcgtt ggtgcaggct attgccgcgc gcgacctgtc gcagcaggtg
     4981 cggcgcgacg ccaccctggt ggaaatcccc gtgcactacg acggcgagga tctggccgag
     5041 gtggcgcaaa tcctgggcat cacgcccgag gagctggtgc gccgccacac aggtagcgac
     5101 tacagcgtgg ccttcgtcgg ctttgcgccg ggctttgcct acctgtcggg cgggcatgag
     5161 agcctggatg tgccgcgccg cgccacgccg cgcacgcgca ttccggcggg cgccgtggcg
     5221 ctggccgggc gcttcagcgg cgtctatccg caggccagcc ccgggggctg gcagatcctg
     5281 ggcaccacgc cgctggccat gtgggacata gagcgcgagc agccggcgct gctgcagccg
     5341 ggttttcgcg tgcgttttgt cgatattgct actctgtctg tagctgttcg cgcacaactg
     5401 gagcgcgctg cagaccaaaa acacttgaag cccgcagcgc ccgcaccagc gcgggggccg
     5461 gcgctggagg tgcgctcgcc cggcctgctc acgctgctgc aagacatggg ccggcccggt
     5521 ttggcggggc agggcgtgtc ggcctccggt gcgctggacc agggcgcctg ccgcacggcg
     5581 aatcggctgg tgggcaaccc acccgacatg gcctgcctgg agaccgtgaa cggcggcctg
     5641 cggctgatga gccatggcga caccgtggtc gccgtgaccg gcgccgatgc gcccctgacg
     5701 cttaccagcg ccggtggcct gcgcaccagc gtgccgcgct accagcccct ggccctggcg
     5761 catggcgatc tgctggccat tggcgagccc gttgccggca cgcgctgcta tgtggcggcg
     5821 cgcggcggct atgacgtggc ccccatcctc ggcagcctga gcaccgacac cctggcccat
     5881 gtcggcccgc cggcgctggc ggcgggcgac cggcttgcct tgcgccccgt ggcgcggggc
     5941 tcgatagtcg gcggcgccga gctgccgccc gatgatctgc ccggccccga tggggaggtg
     6001 gtgctggacg tgatcatggg cccgcgcacc gactggttca cgccccaggc cgtgcagctg
     6061 ctcgccagtc agcgctggcg cgtcacgccc cagtccaacc gcgtgggcct gcgcctggcg
     6121 ggcgagcagc ccctgacccg ctccatcacc caggagctgc ccagcgaggg cacgcccacc
     6181 ggcgccatcc aggtgccggc cagcggccag ccggtgctgt tcctggccga ccacccgctg
     6241 accggcggct accccgtgat cgcctgcatc gcgccgcacc acctcgatcg cgcgggccag
     6301 atccccgtga acgcctgggt gcgcttcaac cccatccggc ccttcgaggc catcaaccgt
     6361 ccttgaatgc catgaaaaaa gtcctcattg ccaatcgcgg agaaaacgac cgcgcagcgg
     6421 gcgtttcatc acattgcggt gcctgcgcag caggcgacgg cgttctcgcc atccagcatt
     6481 tactgaaggc gaaaggtttg aagtcatgaa gaaggttctg attgccaatc ggggcgagat
     6541 cgccgtccgt gtggtgcgcg cctgccgcga ctacggcgtg cagtccgtcg ccgtgtatgc
     6601 caatgccgac atcgacgccc tgcacgcacg catggccgac gaggcctggg ggcttgacgg
     6661 tgaccgcccg gcagacacct atctgcacat agaaaaactg ctggacatcg cccgcaagag
     6721 cggcgccgac gccgtgcacc ccggctacgg cttcctgtcc gagagcgcag cgtttgccca
     6781 ggccgtgctt gatgcgggcc tggcctggat tggccccacg ccccaggcga ttgcgcgcct
     6841 gggcgacaag ctggaggcgc gcaagattgc gctaaaagtg ggcgcgccgc tggtgcaggg
     6901 cacgtcggag ccggcaaaag acgccgccga ggtgctggcc tttgccgagc agcatggcct
     6961 gcccatcatc atcaaggccg cgtttggcgg tggcgggcgt ggcatgaaga tcgcgtggcg
     7021 catggatgag gtggccgagc tgtacgcgtc tgccgtgcgc gaggccacgg cggcctttgg
     7081 ccgcggcgaa tgctttgtcg agcagtttct ggacaagccg cgccatatcg aggcccaggt
     7141 gatgggcgac aagcatggca aggtggtggt gctgggcacg cgcgactgct cgttgcagcg
     7201 gcgcaaccag aagctggtgg aagaagcacc cgcaccgttt ttgaccgacg cccagcgcca
     7261 gcgcatccac caggccgcgc acgacatctg cgccgaggtc ggctacgaga gcgcgggcac
     7321 ggtggaattt ttgctctcgg gcagcggcgc cctatcgttt ttggaggtga acacgcgcct
     7381 gcaggtcgag cacccggtga ccgagcagac caccggcctg gatctggtgc tggagcagct
     7441 gcgcgtggcc gacggtctgc ccctgtccat caccgagacg cccgcgccac tgggccatgc
     7501 gtttgaattc cgcatcaacg ccgaggacgt gggccgcggc tttctgccca cgcccgggcc
     7561 cgtgcagcgc tttgatgcgc ccagcggccc cggcgtgcgc gtggactcgg gcgtgcaggc
     7621 gggctccatc gtgcccggca ctttcgactc gctgatggcc aagctcatcg tcaccggcag
     7681 cacgcgcgag caggcgctgg cacgtgcgcg ccgcgcgctg gccgagtttc agatcgatgg
     7741 cgtggccagc gtgctgccgt ttcaccgggc ggtcatggag caggcggact tcatagccgc
     7801 cgatggcttc aaggtgcata cgcgctggat agaaaccgcc tttgccaacg acctcgcggc
     7861 ggcggtacgt gccgcgcccc aggccgacgc cagcctggtg cgctgcgcca tcgagatcga
     7921 tggccggcgc ctgcaactcg gcctgccggc cgtgctgctg caggggctgg ccgccgccgg
     7981 cggcgcatcg gccactgcgg ccgtcgccca ggagtcggtg gacgaggccg ccgtcgccgc
     8041 ccccatcgcc ggcaacctgc acgcctggaa ggtaggggag ggcgatgccg ttcaggccgg
     8101 cgacgtcatt gccgtcatgg aggccatgaa gatggaaatg caggtcactg cgcaccgctc
     8161 cggccgcatc acgctgcagg cggcggccgg cagctaccag gcggcaggct cttgcatcgc
     8221 gcgcattact tgatggtttg aaggtttttt ggcctctggc ccttataggg taagcgcgag
     8281 cagctatctt ttttgtatca agaggtgtgt gcctgcaggc gcgcaaacag cccgccggcg
     8341 gccaccagct cggcatgcgt gccctgctcg gcaatgcgcc cgcgctccat caccaccacg
     8401 cggtcggcat gctcgatggt ggacaggcgg tgggcgatga ccagggtggt gcgcccgcgc
     8461 atcaggcgct gcagggcgtc ttgcaccagg cgttcggact cggtatcgag cgccgaggtg
     8521 gcctcgtcca ggatcaggat gggcgcgtcc ttgtacagcg cgcgtgcgat tgccaggcgc
     8581 tggcgctgcc cacccgacag ctgcgtggcg ttgtgcccca cgatggtgtg cacgccctgg
     8641 ggcagggcgg ccacatgctc ggccaggttg gcggccgcca ggcagtgctg cacccgctct
     8701 tcgtcgatgg cggcacccag ggccacattg gcggcaatgc tgtcgttgaa catcaccacg
     8761 tcctggctga ccatggcgaa ttgcgcgcgc agcgactcca ggcgccagtc ctgcgcggcc
     8821 acgccgtcga tcagcacctg gccgccgctg gctgccacaa agcgcggcag caggttcacc
     8881 agcgtggtct tgccggcgcc cgacggcccg accagggcca cgatctcgcc ggggcggatg
     8941 tccaggctca cgccgctgag cgcgggcgca tgctcgctgt caaacgacac gttcacatcg
     9001 cgcaggctga tggcgccctg ggcacgctgc acggcatgcg tgccgccggt ttcggccggt
     9061 gcgtcgctga gcagcagcag gccgcgctcc agcgccgcca cgccgcgcgt gatcgggttg
     9121 gccacatcgg ccatgcggcg tatcggcgca atcagcatca gcatggccgc gatgaacgag
     9181 gcaaagccgc ccaccgacac atccttggca ccatggccat ggctttgcca cagggcgatg
     9241 cagatcacgg ccgacagggc gccggcggcc agcagctgcg tggtgggcgt catggccgcc
     9301 gaggcgatgg tggatttgat ggccaggtgg cgcaggcgct ggctcaggtt gttgaagcgc
     9361 gcggcctgac cggcttgtgc gccatgcagg cgcaccatgc ggtgggcgag tacgttctct
     9421 tccaccacat aggccaggtc gtcggtggcc tgctggctgc tcttggtgat cttgtacaga
     9481 cggcgcgaca aggccttcat gatccagccc acgccgggca ccatgacggc cacgatgagc
     9541 gtgagctgcc agttcaaata aatcaggtaa cccagtaacg ccaccagggt gaagccgtcg
     9601 cgcgacacgc caatcagcgc ctgcaccagc gaggtgaagc cggtctgcac ctcatacacc
     9661 acggtgttcg acagggtgct tgccgactgc tgccgaaaca gcgccatgtc ggccgccagc
     9721 agtcgctcga acagggccgt gcgcaggcgc agcatgcctt cgttggcgat gcgcgagagc
     9781 gcatactggc cagtgaactg ggccagtccg cgcaccagga acacaccgat gatggccgtg
     9841 ggaaccatcc acagattcag tgatccctcc gtgaagccct gatcgagcaa gggtttgagc
     9901 agcgcgggta tcagcggctc ggtgatggcg gccaccacgg tggccagcaa ggccatggcc
     9961 caggccagac gctggtaacc aaagtacgca tgcaggcgcc gcagccgggc taacagcgat
    10021 ggtgttggcg gctggggggg cttgggcgcg ctatgattca agtctcgcat gaggggcgga
    10081 ttctacgcag cagcacagtt tgtgacatgg cgcgctacgc tatgtgtaac atcgcaggtt
    10141 ctgttgaccg gcactaaacg gttatttgac ggttattcaa cggttgccaa tgactatcga
    10201 gcgcaccttc ccatcctcac cccttcgcgc ggccatgccg cacatctcgc gccgccatgc
    10261 tgtcgcagca ctggccgccg cctccagcct gccagcactt gcccagttcc gtgtcgagat
    10321 cacgggcgtg ggcctgacgc aactgccgat cgccatcgcg ccgctgcgcg gtgaggcgca
    10381 gtcgccgcag aagatctccg ccatcgtgca ggccgacctg gagcgcagcg gccgctttgt
    10441 cggcgttgac gcctcgggcg tgccgctcga tgaaacctca cgcccggacc tgtcgcagtg
    10501 gcgccagcgc aaggccgacg ccctgaacac cggcagcgtg acgcgtctgg ccgacgggcg
    10561 ctttgacgtg cgcttcaggc tatgggatgt ggtcaagggc caggatctgg gcggacagag
    10621 ctttgtcgtg acgcaggccg acctgcgcct ggtggcgcac cgcatcgcag atttcatcta
    10681 cgagaagctc accggcgaac gcggtgtgtt ctccacgcgc atcgcctatg tcaccaaggt
    10741 gggcgcgcgc tacagcctgt gggtggccga tgccgatggc gaaaatgccc agtcggcact
    10801 gtcaagcccc gagcccatca tctccccggc ctggtcgccc acgggtacgc aattggccta
    10861 tgtgtcgttc gagtcgcgca agccggtggt ctatgtgcac gatgtggcca cgggcaagcg
    10921 ccggctgatc gccaacttcc gcggctccaa cagcgctccc gcctggtcgc ccgacgggcg
    10981 caccctggcc gtcacactga gccgcgacgg tggctcgcag ctctacacca tcgacgcggg
    11041 cggcggcgag ccgcgccgcc tgatgcaaag cgccggcatt gataccgagc cggccttctc
    11101 cagcgacggc cgcagcattt acttcgtgag cgaccgcggc ggctcaccgc agatttatcg
    11161 cgtgggtagc ggtggcggca atgccgagcg cgtgaccttc aatggctcct acaacatctc
    11221 gccggccatc agccccgacg ggcagtggct ggcctatatc tcgcgcatcg gcggcgcctt
    11281 caagctgcag gtcatggatc tgaagtcggg cacggtcagc tcgcttaccg atacctctgc
    11341 cgacgaaagc ccgagctttg cacccaatag ccgcctcatc ctgtacggca cccagcagca
    11401 ggggcgcgag gcgctcatga cgaccaccct ggacggcaag atcaaggcca ggctggccgg
    11461 gcagggcggc gacatccgcg agcccgattg gggcccgttt cagaagtaag tcttcccagc
    11521 tttccaaaca cctgtttttc tcagcacgac atcatgaaat caactccatt caaacgtctt
    11581 tcgatcgcct tcaccattgc cgccctcatg gccggctgca gctccggcgt caagcttgac
    11641 gatgttccgg tcgaggacaa gggcgccacc tccaccaccg gcggtggcgc tggcgccggt
    11701 ggcgcaggac agagcggcgt ggcgccggtg gatctgagcc agtccagccg cgacgtcggc
    11761 ggccccgtcg gtgtggcgcg catcatctac ttcgactttg acagctacac cgtcaagccc
    11821 gagttccagt cgctgctcga agcccacgcg cgttacctga aggcatcctc cgcacgcaag
    11881 gtcatgctcg aaggccacac cgacgatcgc ggtggccgcg aatacaacct ggccctgggt
    11941 cagaagcgtg ccgaggccgt gcgccgttcg ctcggtctgc tgggcgtgtc cgatgcgcag
    12001 gtcgaagccg tgagctacgg caaggaaaag ccagccgtgc agggcaacaa cgaaagcgca
    12061 ttcgcacaga accgccgcgt tgagctgtcg taccgctgat ggccgcgcgc atgtcttttc
    12121 cccaacgctc gcgcgccctg acggtggcag tatttgccgc cgtggcgacc tgggccgtgc
    12181 ccagccatgc gttgttcgag gatgacgagg cgcgccgcgc catcctggag ctgcgtcagc
    12241 gcttcgatgc cttgcagcag tccaaccagc gctctggcga gcgctcgggt gaagaagtct
    12301 cgcaattgcg ccgcagcctg ctcgacctgc aaacgcagat cgaggcattg cgtaccgagc
    12361 aagccagcct gcgcggacaa aacgagcagc tgcagcgtga ggtggcggag ctgcagcgtc
    12421 ggcaaaagga catcgcccaa ggcgtggatg agcggctgcg caagttcgag ccgttggcag
    12481 tgacggtgga tgggcgcgag tttcgtgccg acccggcaga aaagcgcgag ttcgaggcgg
    12541 ctctggccgt gttccgctcg ggcaaatttc ctgaggcggc aacggcgttc ggcagcttta
    12601 tgcgccagta cccgcagtcc ggctacctgc catcggcacg cttctggctg ggcaatgcgc
    12661 aatacgccac gcgagactac aaagaggcta tcaacaactt caagagcctg ctggcagccg
    12721 cgcccgacca tgcgcgcgcg cccgaggcag cattgtcaat tgccaactgc cagatcgagc
    12781 tgaaggacac gcgcgcagcg cgcaagacgc tggaagacct gatgcgcgta tatccgcaat
    12841 ccgaagccgc agccgctgcc aaggaacgtc tggcgcgcct gaagtgagcg tgccggcgca
    12901 agagtcggcc gatctgcagc gccgctttgg cggtctgcag cggctgtatg gcgtggccgg
    12961 cgcccagcgc atccgcgccg cgcatgtcgc cgtcatcggt atcggcggcg tcggttcctg
    13021 ggccgcggaa gccctggccc gctccggcgt ggggcgcctc accctgatcg acatggatca
    13081 tgtggccgaa tccaacgtca accgtcagat acacaccctg agcaccaccc tcggacaagc
    13141 caaggtgcgc gccatggccg agcgcattgc gctcatcaac ccagagtgcc gggtcgattg
    13201 catagaagag ttcgtggatc cggccaactg gccaaccatc gtgcccgccc cggtggatgc
    13261 cgtcatcgat gcttgcgatc aggttcgcgc caaggccgcc atggctgcct gggccatgca
    13321 aacgcgtacc gctttcatca ccgtgggcgc ggccggcgga aagcgactgg cgcacaaggt
    13381 cgatgtggat gacctggcac acaccacaca tgacccgttg ctggctcagt tgcgctatcg
    13441 cctgcgccgt gaacatggcg ccgcccgcga cggcaagcgc atcggcgtgg cctgcgtctt
    13501 cagccgcgaa gccgtggccc cgccgcatgc ctcctgcgcc atcgatgaag gcgatggttc
    13561 attgaactgc catggctacg gctccagcgt ggccgtcacc gccaccttcg ggcattgcgc
    13621 ggccggctgg ttgctggaca aactgtcggc ggacgttcca aaaaacacgc tataatctac
    13681 gactttgctg tagacatgca gcaaagcctg ggacgttagc tcagttggta gagcagcgga
    13741 cttttaatcc gttggtcaca agttcgaatc ttgtacgtcc taccaagcta t
//
LOCUS       DAOMTQ010000079        13670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_757_length_13670_cov_28.370327, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000079 DAOMTQ010000000
VERSION     DAOMTQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13670
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_757_length_13670_cov_28.370327"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PK518_09050"
     CDS             <1..963
                     /locus_tag="PK518_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRM49102.1"
                     /translation="LGINPTYVDGTMIGGSSFIAHLLPAMRALEAGQCKAVLVCYGSA
                     QRSATFGRKESVAARRFLDPQPYEFPYEPVLPVTAYALAAARHMHEFGTTRAQLAEVA
                     VAARAWAQMNPEAFMRDPLTIEDVLGARPIASPLTVRDCCLVTDGGGAIVLTRADRAR
                     DLARPPVYVLGNATAIWNRQISCMPDLTTTSAAQSGAQAFAMAGLKAADMDMAQVYDA
                     FTINTILFLEDLGFCAKGEGGAFVAKGGIAPGGRLAVNTNGGGLSCVHPGMYGIFALI
                     EAVRQLRGEAGARQLKQHRTAVVHGNGGTLSSQSTAILGTAETL"
     gene            974..2131
                     /locus_tag="PK518_09055"
     CDS             974..2131
                     /locus_tag="PK518_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM49103.1"
                     /translation="MIRDPEILEALLDSVRRFVRERLVPAENEVAETDEIPESIVQEM
                     RELGLFGMTIPEKFGGLELTMEEECRVLLELCQTAPAFRSVIGTTVGIGSQGILMDGT
                     PEQQAEWLPKLATGEVIASFALTEPEAGSDAASLRTTAIKDGDHYVVNGTKRFITNAP
                     HAGMFTLMARTDPTNKGAGGVSSFIVDAKSPGISFGKYDKKMGQKGAHTCDVIFDNVK
                     VPAANLIGLKEGQGFKTAMKVLEKGRIHIAAVAVGVAKRMLRDALAYSLERKQFGHAI
                     CDFQLVQAMLADSQAELYAAECMTLDAARRRDDGQNVSTEASCAKMFATEMCGRVADR
                     AVQILGGAGYMEEYGIERFYRDVRLFRLYEGTTQIQQIIIARNMVREARDA"
     gene            2128..2550
                     /locus_tag="PK518_09060"
     CDS             2128..2550
                     /locus_tag="PK518_09060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRM49104.1"
                     /translation="MNGAAPPPDRSAFMRLIGAAQVPAPPGKVHVRLPHMREELLNQL
                     PAAHGGVIMTLLDSVMSRACALQPGAPSQTAVTVEMTSRFHRPGRGALLAEGWVVHST
                     RSLCSCAAQLLDEEGRLVATASGTFKYWLAPTTLDSAE"
     gene            2721..5153
                     /locus_tag="PK518_09065"
     CDS             2721..5153
                     /locus_tag="PK518_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRM49105.1"
                     /translation="MATAKPRSRTGKNSKANPKEAGGKTAGFNASNPFSELERAASGQ
                     VQAFPAILSKEGSTPEVGTVVYVHGIGNKPKCSVLKCQWDTALFGASMGDRTRMAYWV
                     DRNRYPQPVEGSCADGDRLLDAADAVGLQALADIGLEREPVLDAAARKFMDALEARLR
                     GNEQETGGVDAKLLPLPEVTRLWITRRITKLFLKDVQDFFFDARKRALMEQALRERLD
                     AGGGPFIVIGHSQGSMIAYQVLRQLQKADCDVRLFVTIGSPLGLQEVKDGLRKIGQGK
                     PLVVPECVDRWLNVAERLDPVALDAHLANDYKANSRGVKAVDDSRLMINPDWKTNPHS
                     GTGYLSIDIVRNTVREAAGPSFSNPVGRNILMKDLVADIEDGQREQRHPTLIQLVSDA
                     GDDTPLAQVRDRLEGLIRQVLQFHGATADDGRIQVMQRYISADLTRSEIEQLRSHCGT
                     LKIDRVWRNAVKRALLNQSTHTLQVRPANLGYGACGRDIGWAVLDTGISAAHPHFKEH
                     ANVVAQWDCTLTGSPRPLKPGDALFGTLDGNGHGTHVAATIAGGMTLPNDDGDASAVA
                     MLGMAPEARLYGFKVLKDSGSGEDAFIIKALDQIAELNERAGKLVIHGINLSLGGSFD
                     PSVFGCGHTPMCQELRRLWRQGVLVCLAAGNEGYALLDSANGAIAANMDLSIGDPANL
                     EEAIAVGSVHKTNPHTYGVSYFSSRGPTADGRMKPDLVAPGENILSARHQWPKAKPTV
                     RNAYVQMSGTSMATPHVSGLLAAFLSARREFIGYPERVKALLLAHCTDLGRDPYIQGK
                     GMPNLVRMIMNT"
     gene            complement(5189..6103)
                     /locus_tag="PK518_09070"
     CDS             complement(5189..6103)
                     /locus_tag="PK518_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM49106.1"
                     /translation="MAMHFDLVDLRLMVHIADANSMTRGAELSFISLPAASTRIKNLE
                     ESIGTKLLYRTSQGVTLTPPGQAFMTHARMVLSQIEHLRGDMQEYVRGIKGHVRVFAN
                     TTSSGEFLPPVLRHFLRQHPDVNIDLRERLSHDIVRAVTEGQTDIGIVAGLVRTENLE
                     TLPYRRDRLVLVLPRGHALDGQMQIAFADTLDLDYVGLHESSAIHAFLRQASDQLHRP
                     IKQRIQVSNFETACRMIEAGVGVGVLPESAASRHAQSMDIAIVPLADAWSVREMQICV
                     RSLEALPSFAQELVQMLVDDAQGRLALG"
     gene            6228..7499
                     /locus_tag="PK518_09075"
     CDS             6228..7499
                     /locus_tag="PK518_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRM49107.1"
                     /translation="MSQSDSSPTAGALSHLRVLDLSRVLAGPWCTQNLADMGADVIKI
                     EKPGEGDDTRHWGPPFFPDAQGQPTSNACYFASCNRNKRSVTVDMATPEGQELIRELA
                     RQSDVVVENFKTGGLARYGLDYASLSALNPRLIYCSVTGFGQTGPYAPRAGYDLLIQA
                     MSGMMSITGRADGEPGGGPLRVGVAVIDLFTGMYATTAILGALEARHRTGRGQHIDMA
                     LLDVAMAMLANQGAGFLNAGNIPKRQGNTHPSVVPYQDFPTQDGNMLLAIGNDGQFAR
                     FCEAAGVDWASDERFTLNAGRVIHRETLIPMMAELTRTRPTAAWIKLLEDKAVPCGPI
                     NHVGQAFDDEQVRARGLRIEQDRYPDGQLPAGESINRVVSTASPLRLSDTPVTLRHAP
                     PALGQHTDEVLAERLGLDAARLQELRAKGVV"
     assembly_gap    7546..7645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7711..9003)
                     /locus_tag="PK518_09080"
     CDS             complement(7711..9003)
                     /locus_tag="PK518_09080"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRM49108.1"
                     /translation="MTTPASTHALPSYLDPNHLGPWGVYLQQVDRVTPYLGSLARWVE
                     TLKRPKRALIVDVPIEMDNGTIAHFEGYRVQHNLSRGPGKGGVRFHQDVTLSEVMALS
                     AWMSIKNAAVNVPYGGAKGGIRVDPKKLSRSELERLTRRYTSEIGIIIGPSKDIPAPD
                     VNTNAQVMAWMMDTYSQNVGATSTGVVTGKPVDLGGSLGRVEATGRGVFTVGCEAARL
                     TGMAIEGARVAVQGFGNVGGTAGKLFAEAGAKVVAVQDHTGSIHNAKGLDVPALLAHV
                     QQTGGVGGFAGADAMANDEFWGVACDILIPAALESQITQDNAGKIQAKMVIEGANGPT
                     TPEADDILHDKGVLVVPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINARLVRIMKEA
                     FAGVWQVAQDNKVSLRTATFIVACKRMLHAREMRGLYP"
     gene            9190..9654
                     /locus_tag="PK518_09085"
     CDS             9190..9654
                     /locus_tag="PK518_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HRM49109.1"
                     /translation="MKTSPSATGLQGPVRRFVFVTLYELIAIIVSSVLFMAIGLEAGA
                     SGVMAVVASTLAIVWNVTFNHLFERWEARQTVKGRSVRRRAVHAMGFEGGLALMLIPL
                     MAWWFEVSLWQATWMEAGLLLFFLVYTYAFNWAFDHIFGLPASAQPASLPVA"
     gene            complement(9709..11118)
                     /gene="ccoG"
                     /locus_tag="PK518_09090"
     CDS             complement(9709..11118)
                     /gene="ccoG"
                     /locus_tag="PK518_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRM49110.1"
                     /translation="MTTPPPPSTPAKYIRLYEAKQKVYPRAIDGVFNRWRWVLLVITQ
                     LVFYGLPWLQWGGRQMVLFDLGARRFYLFGLVLYPQDFIYLTGLLIISALSLFLFTAV
                     AGRLWCGFACPQTVYTKLFMWLETHIEGDRSARMRLDKAPWTSWEKLWRKGLKQLTWI
                     SVALWTGFSFVGFFVPIRQLGAELLALHGSWQLFWIGLYSLATYGFAGYMREQVCKYM
                     CPYARFQSAMFDKDTLVVTYDSQRGEPRGARSKKVVDYKAAGLGDCIDCSLCVQVCPT
                     GIDIRNGLQYECIGCGLCVDACDSVMDKVQYPRGLIRFATQNGMAQRWSSAQMLRRVL
                     RPRVLLYGSVLLALSIAMLISLATRIPLKVDVVRDRAALARIVAGGQLENIYRLQIMN
                     ATEGVQRYRIASRGLEGLHVATDNEVEIGPAESRWVVVRLQLPFGAAPQGSHTVHFDV
                     QALGDGAQVSEKSVFLVPR"
     assembly_gap    11346..11445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11446..12779
                     /locus_tag="PK518_09095"
     CDS             <11446..12779
                     /locus_tag="PK518_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HRM49111.1"
                     /translation="QPALLAAWLEALPEPHIVFDHDYRIVAANSAYRRQFGHGAQVVG
                     RTCHAVSHHSPVPCDQAGETCPLARARLSGQRERVLHLHHTPAGEEYVQIELVPLRGE
                     SGQPMYFMEKMEPLRIAQGRSGAQGMIGRAPAFKALLALIARVGPSQASVLLLGESGT
                     GKELAARAVHQASARAARPLVVVDCASLPETLFESELFGHERGAFTGAHAARAGLVEA
                     AHGGTLFLDEVGDIPLTMQVKLLRLLESGTYRRVGQSELRRADIRLVAATHRDLQAMV
                     REGRFRQDLYYRLSTFPIRLPALRERVQDIALLAESLLARVAPGRQLALAPAALRRLM
                     QHPFAGNVRELRNVLERAALLTDGSTITLQTVEQALAFDVQPDTSVATPAGTPATGRA
                     TLRSMEDDILRAALRAPAGTRQEQARALGISPRTLYRKLRQLQEGETRFSQ"
     gene            complement(12769..13266)
                     /locus_tag="PK518_09100"
     CDS             complement(12769..13266)
                     /locus_tag="PK518_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49112.1"
                     /translation="MLSARRLAQGDPGLLAAIERSQRLLHRRALVAGAASALPVPGLD
                     WAVDAALLSRLIPAINHHFGLTPQQIDRLDPRERDKLQKAIAQVGALLVGKFITQDLL
                     LRSARLIGVRLTTVQVARFVPLVGQAASAAIGYGAIRFLGQQHIQDCVRVVRQAGLAL
                     PPPTD"
     gene            complement(13277..13522)
                     /locus_tag="PK518_09105"
     CDS             complement(13277..13522)
                     /locus_tag="PK518_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRM49113.1"
                     /translation="MLNLLGTLFIGLIVGWVARFLLPGKQALGLIMTSLLGVAGSFIA
                     TYAGQFLGLYPAGAAAGFIASVVGAFVLLLVATKVLK"
BASE COUNT         2288 a         4585 c         4511 g         2086 t
ORIGIN      
        1 ctgggcatca acccgaccta tgtggacggc accatgatcg gcggctccag cttcatcgcc
       61 cacctgctgc cggcgatgcg cgcgctcgag gccgggcagt gcaaggcggt gctggtgtgc
      121 tacggcagcg cccagcgctc ggccaccttc gggcgcaagg aaagcgtggc cgcgcggcgc
      181 tttctcgacc cccagcccta cgagtttccc tacgagccgg tgctgccggt gacggcctat
      241 gcgctggccg cggcgcgcca catgcatgag ttcggcacca cgcgcgcgca actggccgag
      301 gtggccgtgg ccgcacgcgc ctgggcccag atgaaccccg aggccttcat gcgcgatccg
      361 ctcaccatcg aggacgtgct cggggcgcgc cccatcgcca gcccgctgac ggtgcgcgac
      421 tgttgcctgg tgaccgacgg cggcggcgcg atcgtgctca cgcgcgccga ccgggcacgc
      481 gacctggcgc ggcccccggt ctatgtactg ggcaatgcca cggcgatctg gaaccgccag
      541 atctcctgca tgccggattt gacgacgacc agtgccgcgc agtccggcgc ccaggccttt
      601 gcgatggcgg gcctgaaggc ggccgacatg gacatggccc aggtctacga cgccttcacc
      661 atcaacacca tcctgttcct ggaagacctg ggcttctgcg ccaagggcga gggtggcgcc
      721 ttcgtggcca agggcggcat cgcccccggc ggacgcctgg ccgtgaacac caatggcggc
      781 ggcctgtcct gcgtgcaccc cggcatgtac ggcatctttg cgctcatcga ggccgtgcgc
      841 cagctgcgcg gcgaggccgg tgcgcgccag ctgaagcagc accgcacggc cgtggtgcac
      901 ggcaacggcg gtacgctgtc gagccagtcc acggccattc tcggcaccgc cgaaaccctg
      961 taaggagaca cccatgatcc gcgacccaga aatattggaa gccctgctgg acagcgtgcg
     1021 ccgtttcgtg cgcgagcgcc tggtgccggc cgagaacgaa gtggccgaga ccgacgagat
     1081 ccccgaatcc atcgtgcagg agatgcgcga gctgggcctg ttcggcatga ccattccaga
     1141 aaaattcggc ggcctggagc tgaccatgga ggaggaatgc cgtgtgctgc tggagctttg
     1201 ccagaccgcg ccggcctttc gctccgtcat cggcaccacg gtgggcatag gctcgcaggg
     1261 catcctgatg gacggcacgc ccgagcagca ggccgagtgg ttgccgaagc tggccacggg
     1321 cgaggtcatt gcctcgttcg cgctcaccga gcccgaggcc ggctcggacg ccgcctcgct
     1381 gcgcaccacg gccatcaagg atggcgacca ttacgtcgtc aacggcacca agcgcttcat
     1441 caccaacgcg ccgcacgccg gcatgttcac gctgatggcg cgcaccgacc cgacgaacaa
     1501 gggcgcgggc ggcgtgtcct ccttcatcgt cgatgcgaaa agccccggca tcagcttcgg
     1561 caagtacgac aagaagatgg gccagaaggg cgcacacacc tgcgacgtga tttttgacaa
     1621 cgtcaaggtg ccggccgcca atctgatcgg cctgaaggaa ggccagggct tcaagacggc
     1681 gatgaaggtg ctggagaagg gccgcattca catcgccgcc gtggccgtgg gtgtggccaa
     1741 gcgcatgctg cgcgacgcac tggcttattc gctggagcgc aagcagttcg gccacgccat
     1801 ctgcgacttc cagctggtgc aggccatgct ggccgacagc caggccgagc tgtacgccgc
     1861 cgaatgcatg acgctggacg ccgcgcgccg gcgcgacgat gggcagaacg tgtccaccga
     1921 ggcctcctgc gccaagatgt tcgccaccga gatgtgcggc cgcgtggccg accgcgcggt
     1981 gcagatcctg ggcggcgctg gctatatgga ggagtacggc atagagcgct tctaccgcga
     2041 cgtgcgcctg ttccgcctgt acgagggcac gacgcagatc cagcagatca tcatcgcgcg
     2101 caacatggtg cgcgaggcgc gcgacgcatg aacggggccg caccgccccc cgaccgcagc
     2161 gccttcatgc gcctgattgg cgcggcccag gtgccggcgc cgccgggcaa ggtgcatgtg
     2221 cgcctgcccc acatgcgtga agagctgctg aaccagctgc ccgccgcgca tggtggcgtg
     2281 atcatgaccc tgctggactc ggtcatgtcg cgcgcctgcg cgctgcagcc cggcgcgcca
     2341 tcgcagaccg ccgtgacggt ggagatgacg agccgctttc accgcccggg gcgcggcgcg
     2401 ctgctggccg aaggctgggt ggtgcactcc acccgcagcc tgtgtagctg cgcggcgcag
     2461 ctgctggacg aagaaggccg gctggtggcc acggccagcg gcaccttcaa gtactggctg
     2521 gcaccgacca cgctggattc ggccgaatag cccgattgcc aaatgcaaca gcggttgctg
     2581 ctgttgccgc cttggaatac aaaagccgtc ggtggtagag ctatggcggg gtgggcggca
     2641 acaagtggct tgcagcatgc ttgcggtttc cagtgaggcc ggtgccgatt ccagttccgc
     2701 accgacaagg agctggaatc atggcgacag cgaaaccgcg ttcacggacc ggaaaaaatt
     2761 ccaaggccaa tcccaaggag gctggtggca agacagccgg cttcaatgcc tccaatccct
     2821 tcagcgagct tgaacgggcc gcgagcggcc aggtacaggc gtttccggcc atcctcagca
     2881 aggagggcag cacgccggaa gtcggcaccg tggtgtatgt ccatggcata ggcaacaagc
     2941 ccaaatgctc ggtgctcaag tgccagtggg acacggcgct gttcggcgcc tccatgggcg
     3001 atcgcacccg catggcctac tgggtggatc gcaatcgcta tccccagccg gtggagggca
     3061 gttgcgccga tggcgacagg ctgctggatg ctgccgatgc cgtcggcttg caggcgctgg
     3121 ccgacatcgg gctggagcgg gagccggtac tcgacgccgc ggcacgcaag ttcatggacg
     3181 ccctggaggc gcggctgcgt ggcaacgagc aagagaccgg cggcgtggac gccaagctgc
     3241 tgcccttgcc ggaagtcacg cggttgtgga tcacgcgccg catcaccaag ctgttcctca
     3301 aggacgtgca ggactttttc ttcgacgccc gcaagcgcgc cctgatggag caggcgctgc
     3361 gcgagcgcct ggacgcgggg ggcgggccct tcatcgtcat cggccacagc cagggctcga
     3421 tgattgccta ccaggtgctg cgccagttgc agaaggccga ttgcgacgtg cgcctgttcg
     3481 tcaccatcgg ctcgccgctg ggactgcagg aggtcaagga tgggctgcgc aagatcggcc
     3541 agggcaagcc gctggtcgtg ccggaatgcg tcgatcgctg gctgaatgtg gccgaacggc
     3601 tcgaccccgt ggcgctggat gcgcatcttg ccaacgacta caaggccaac agccgcggtg
     3661 tgaaggccgt ggatgactca cgcctgatga tcaaccccga ctggaagacc aacccgcatt
     3721 ccggcactgg ctatctttcc atcgacatcg tgcgcaatac cgtgcgcgag gctgccggcc
     3781 cctcgttcag caatcccgtg ggccgcaaca tcctgatgaa ggatctggtg gccgacatcg
     3841 aggacggcca gcgcgagcag cgccacccga ccctgatcca gctggtgtcc gatgccggtg
     3901 atgacacgcc gctggctcag gtgcgcgacc ggctggaggg gctgatcagg caggtgctgc
     3961 agttccacgg cgccacggcc gacgatggcc gcatccaggt catgcagcgc tatatctccg
     4021 ccgacctgac gcgcagcgag atcgagcagc tgcgctcgca ctgcggcacg ctgaagatag
     4081 accgtgtctg gcgcaatgcc gtcaagcgcg ccctgctgaa ccagtccacg cacacgctgc
     4141 aggtgcggcc ggcgaatctg ggctatggcg cctgcggcag ggacatcggc tgggccgtgc
     4201 tggacaccgg catttctgcc gcccatccgc atttcaagga gcatgcgaat gtcgtcgcgc
     4261 aatgggactg cacgctcacc ggcagcccca ggccgctcaa gcccggtgac gccctgtttg
     4321 gcacgctgga cggcaacggc cacggcacgc atgtggcggc caccattgcc ggaggcatga
     4381 cgctgccgaa cgatgacggc gatgccagcg ccgtcgccat gctgggcatg gcgcccgagg
     4441 cgcggctcta cggcttcaag gtgctcaagg acagtggcag cggcgaggac gccttcatca
     4501 tcaaggcgct ggaccagatc gccgagctga acgagcgcgc cggcaagctt gtcatccatg
     4561 gcatcaacct gagcctgggc ggcagtttcg accccagcgt cttcggctgc ggccataccc
     4621 cgatgtgcca ggagctgcgc cggctctggc gccagggcgt gctggtatgc ctggccgcgg
     4681 gcaacgaggg ctatgcgctg ctcgactccg ccaatggcgc catcgccgcg aacatggatc
     4741 tgtcgattgg cgacccggcc aatctggagg aggccatcgc cgtcgggtcg gtgcacaaga
     4801 ccaatccgca cacctatggc gtgtcgtatt tttcgtcgcg cgggcccacg gccgacgggc
     4861 gcatgaaacc cgacctggtg gcgccgggcg agaacattct ctcggcgcgc caccaatggc
     4921 ctaaagccaa gcccaccgtg cgcaacgcct atgtgcagat gagcggcacc agcatggcca
     4981 cgccgcatgt ctcggggctg ctggcggcct ttctgtcggc gcggcgcgag ttcatcggct
     5041 acccggagcg cgtcaaggcg ctgctgctgg cgcattgcac cgacctgggc cgcgacccct
     5101 acatccaggg caagggcatg ccgaacctgg tgcgcatgat catgaatacg tgagggcgcc
     5161 gcggacggcg ccgcccatca gcccagagtc agcccagcgc cagccgcccc tgcgcgtcat
     5221 cgaccagcat ctgcaccagc tcctgcgcaa agctgggcag ggcctccagg ctgcgcacgc
     5281 agatctgcat ctcgcgcacc gaccaggcgt cggccagcgg cacgatggcg atgtccatgg
     5341 actgcgcatg gcggctggcg gccgactcgg gcagcacgcc cacgccgacg ccggcctcga
     5401 tcatgcggca ggcggtctcg aagttgctca cctgtatgcg ctgcttgatg ggccggtgca
     5461 gctggtcgct ggcctggcgc agaaaggcgt ggatggcgct cgattcgtgc aggcccacat
     5521 agtccagatc cagcgtgtcg gcaaaggcga tctgcatctg gccgtccagc gcgtggccgc
     5581 gcggcagcac cagcaccagg cggtcgcggc ggtagggcag ggtctccagg ttctcggtgc
     5641 gcaccaggcc ggcgacgatg ccgatgtcgg tctgcccctc ggtcacggcg cgcacgatgt
     5701 cgtgcgacag gcgctcgcgc aggtctatgt tcacgtccgg gtgctggcgc aaaaagtggc
     5761 gcagcaccgg cggcagaaac tcgcccgacg aggtggtgtt ggcaaacacg cgcacatggc
     5821 ccttgatgcc gcgcacatat tcctgcatgt cgccgcgcag gtgctcgatc tgcgagagca
     5881 ccatgcgcgc gtgcgtcatg aaggcctggc ccggtggcgt cagcgtcacg ccctggctgg
     5941 tgcggtagag cagcttggtg ccaatgctct cctccaggtt cttgatgcgc gtgctggccg
     6001 cgggcaggga gatgaaggac agctcggcgc cacgcgtcat gctgttggcg tcggcgatat
     6061 gcaccatcag gcgcaagtcc acaaggtcga aatgcattgc catggcgggc attgtaggaa
     6121 gcgcggcgag ggtggcggat acgcaaatcc ccgctttcac aaaagcgaag ccggcgtggt
     6181 gcggctcgcc ttaccatgcc gccttgtctg tccaacgcca ttcgcccatg tcccaatctg
     6241 attcctcccc caccgccggc gccctgagcc atctgcgcgt gctcgacctg tcgcgcgtgc
     6301 tggccggccc ctggtgcacg caaaacctgg ccgacatggg cgccgacgtc atcaagatag
     6361 aaaagcccgg cgagggcgac gacacgcgcc actggggccc gcccttcttc cctgacgcgc
     6421 agggccagcc caccagcaat gcctgctact tcgcgtcctg caaccgcaac aagcgctccg
     6481 tcaccgtgga catggccacg cccgagggcc aggagctgat ccgcgagctg gcccggcaaa
     6541 gcgacgtggt ggtggagaac ttcaagaccg gggggctggc gcgctacggc ctggattacg
     6601 cgagcctgtc ggcgctcaac ccgcgcctga tctactgctc ggtcaccggc tttggccaga
     6661 ccgggccata tgcgccgcgc gccggctacg acctgctgat ccaggccatg agcggcatga
     6721 tgagcatcac cggccgcgcc gatggcgagc ccggcggcgg cccgctgcgc gtgggcgtgg
     6781 cggtgatcga cctgttcacc ggcatgtacg ccaccaccgc catcctgggt gcactggagg
     6841 cgcgccaccg caccgggcgc ggccagcata tcgacatggc gctgctggac gtggccatgg
     6901 ccatgctggc caaccagggc gcgggcttcc tgaacgccgg caacatcccg aagcgccagg
     6961 gcaacacgca ccccagcgtc gtgccctacc aggacttccc cacgcaggac ggcaacatgc
     7021 tgctggccat aggcaatgac ggccagttcg cgcgcttttg cgaggccgcg ggcgtggact
     7081 gggcgagcga cgagcgcttc acgctgaacg ccggccgtgt catccaccgc gaaacgctga
     7141 tcccgatgat ggccgagctc acccgcaccc gccccacggc cgcctggatc aagctgctgg
     7201 aggacaaggc cgtgccctgc ggccccatta accatgtggg ccaggcgttt gacgacgagc
     7261 aggtgcgcgc gcgcggcctg cgcatcgagc aggatcgcta ccccgacggc cagctgccgg
     7321 ccggcgagag catcaaccgc gtggtcagca cggccagccc gctgcgcctg tccgacaccc
     7381 ccgtcaccct gcgccacgcc cccccggccc tgggccagca cacggacgag gtgctggcag
     7441 agcgcctggg gctggacgcg gcgcgcttgc aggagctgcg cgccaagggg gtggtgtgag
     7501 ggggcgttga gcgtttggcg tccagccaga gaccacgctc aacgcnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnaacgc tcaacgctca acgctcaaca aaaaaagcgg
     7681 ccgaagccgc ttttttgggg ggtgagccga tcagggatac aggccgcgca tctcgcgggc
     7741 atgcagcatg cgcttgcagg ccacgatgaa ggtggccgtg cgcaacgaca ccttgttgtc
     7801 ctgtgccacc tgccagacgc cggcgaaggc ctccttcatg atgcgcacca ggcgggcgtt
     7861 gatctcctcc tcggtccaga agaagctgga gaagtcctgc acccactcga agtagctcac
     7921 cgtcacgccg ccggcgttgg cgatcacgtc gggcaccacc agcacgccct tgtcgtgcag
     7981 gatgtcgtcg gcctcgggcg tggtggggcc gttggcgccc tcgatgacca tcttggcctg
     8041 gatcttgccc gcgttgtcct gggtgatttg gctctccagc gcggcgggga tcaggatgtc
     8101 gcaggccacg ccccagaact cgtcattggc catggcgtcg gcgccggcaa agccgcccac
     8161 gccgccggtc tgctgcacat gcgccagcag agccggcacg tccaggccct tggcgttgtg
     8221 gatgctgcca gtgtggtcct gcaccgccac caccttggcg ccggcctcgg caaacagctt
     8281 gccggccgtg ccgcccacgt tgccaaagcc ctgcaccgcc acgcgtgcgc cttcaatggc
     8341 catgccggtc aggcgcgcgg cctcgcagcc cacggtgaac acgccgcggc cggtggcttc
     8401 cacgcggccc agcgagccgc ccaggtccac gggcttgccg gtgaccacgc cggtggaggt
     8461 ggcgccgacg ttctgcgaat aggtgtccat catccaggcc atgacctggg cgttggtgtt
     8521 cacgtcgggc gcggggatgt ccttggacgg gccgatgatg atgccgatct cgctggtgta
     8581 gcggcgcgtc aggcgctcga gctcgctgcg cgagagcttc ttggggtcaa cgcggatgcc
     8641 gcccttggcg ccgccgtagg gcacgttcac ggccgcgttc ttgatcgaca tccaggccga
     8701 cagggccatc acctcggaca gcgtcacgtc ctggtggaag cgcacgccgc ccttgcccgg
     8761 gccgcggctc aggttgtgct gcacgcggta gccctcgaag tgggcgatgg tgccgttgtc
     8821 catctcgatc ggcacgtcga cgatcagcgc gcgcttgggg cgcttgagcg tctcgaccca
     8881 gcgcgccagg ctgcccaggt agggcgtgac gcgatcgacc tgctgcaggt agacgcccca
     8941 ggggccgagg tggttgggat cgaggtagga gggcagggca tgtgtgctgg cgggggtggt
     9001 catggttttg tccttgtgaa acaaatcaag aagcgagact gtatgcctgt ggtgtcgcgc
     9061 tgtccaacac ccatctgtat gcctgtaatg cgtggcacgc atagaccctg ataccggccc
     9121 tggaaattgc atggcccggg ttaacccttg accagcaagg cgtggcgccc cctacctata
     9181 ctgccggcca tgaaaacaag cccttcggcc accggcctgc aaggcccggt gcgccgtttc
     9241 gtatttgtca cgctctacga gctgatcgcc atcatcgtct ccagcgtgct gttcatggcc
     9301 attggcctgg aggcgggcgc ctcgggcgtg atggcggtgg tggcgtccac gctggccatt
     9361 gtctggaacg tcaccttcaa ccacctgttc gagcgctggg aggcacgcca gacggtcaag
     9421 ggccgctcgg tgcgccgccg cgcggtgcat gccatgggct tcgagggcgg gctggcgctg
     9481 atgctgattc cgctgatggc ctggtggttc gaggtcagcc tgtggcaggc gacatggatg
     9541 gaggccgggc tgctgctgtt cttcctggtc tacacctacg ccttcaactg ggccttcgac
     9601 catatcttcg gcctgccggc ctcggcgcag ccagcaagcc tgccagtagc atgatatttt
     9661 ggtagctgtc agcgcttgat ggataagcgc tacagcctgt tttgatgcct atcttggcac
     9721 caaaaacact gacttctccg acacctgcgc cccgtcgccc agcgcctgca catcaaagtg
     9781 cacggtgtgc gaaccctggg gcgccgcgcc gaagggcagc tgcagccgca ccacgaccca
     9841 gcgcgactcg gccgggccta tctccacctc gttgtccgtg gccacatgca ggccctccag
     9901 gccgcgggac gcgatgcggt agcgctgcac gccctcggtg gcgttcatga tctgcaggcg
     9961 gtagatgttc tccagctggc cgccggcgac gatgcgcgcc agcgccgcgc ggtcgcgcac
    10021 cacgtccacc ttcagcggga tgcgcgtggc aaggctgatg agcatggcga tgctcagcgc
    10081 cagcagcacg ctgccgtaca gcagcacgcg tgggcgcagc acgcggcgca gcatctgcgc
    10141 gctgctccag cgctgggcca tgccgttctg cgtggcaaag cggatcagcc cgcgcgggta
    10201 ctgcaccttg tccatgaccg agtcgcaggc gtccacgcac aggccgcagc ctatgcactc
    10261 gtactgcagg ccgttgcgaa tgtctatgcc cgtgggacag acctgcacgc acagcgagca
    10321 gtcgatgcag tcgcccaggc ccgcggcctt gtagtccacc accttcttgc tgcgcgcgcc
    10381 gcggggctcg ccacgctggc tgtcgtaggt gacgaccagc gtgtccttgt cgaacatggc
    10441 gctctgaaaa cgcgcgtagg ggcacatgta tttgcacacc tgctcgcgca tgtagccggc
    10501 aaagccatag gtcgccaggc tgtacagccc gatccagaac agctgccagc tgccgtgcag
    10561 ggccagcagc tcggcgccga gctggcgtat gggcacgaag aagccgacaa agctgaaacc
    10621 cgtccacagc gccacgctga tccaggtgag ctgcttgagg cccttgcgcc agagcttttc
    10681 ccatgacgtc cagggcgcct tgtccaggcg catgcgtgcg ctgcggtcgc cctcgatatg
    10741 tgtctccagc cacatgaaga gcttggtgta caccgtctgc gggcaggcaa aaccgcacca
    10801 caggcgcccg gccacggcgg tgaacagaaa cagcgacagc gccgagatga tgagcagccc
    10861 ggtcaggtag atgaagtcct gcggatacag caccaggcca aacaggtaga agcgccgtgc
    10921 ccccaggtcg aacagcacca tctgccgccc gccccattgc agccagggca ggccgtagaa
    10981 caccagctgg gtgatgacca gcagcaccca gcgccagcgg ttgaacaccc cgtcaatggc
    11041 gcgtggatag accttctgct tggcctcgta caggcgtatg tacttggcgg gcgtggaggg
    11101 cggcggaggt gtggtcatgt ggagcctgta tggcgggcaa gcgcgttgcg gttgcttgtc
    11161 atgaatcaag actcgtgcca gcccggcgct gtcagggctt tgagaaaaaa cactgcggca
    11221 gcaagcactt agccatcggt cgtccgtgta tgagtgacat gaatggcagg agacactgac
    11281 atttgctgtc atgtatgtca ccatctgaca ccatgaacga cagcaccagc gacgactcca
    11341 cccccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgcaa cccgcgctgc
    11461 tggcagcgtg gctggaggcc ctgcccgagc cgcacatcgt ctttgatcac gactaccgca
    11521 tcgtcgccgc caacagcgcc taccggcgcc agttcggcca tggcgcgcag gtcgtggggc
    11581 gcacctgcca cgcggtgtcg caccactcgc ccgtgccctg cgaccaggcg ggcgaaacct
    11641 gcccgctggc gcgcgcccgg ctcagcggcc agcgcgagcg cgtgctgcat ctgcatcaca
    11701 cgcccgcggg tgaggaatat gtgcagatcg agctcgtgcc gctgcgcggc gaatccggcc
    11761 agccgatgta cttcatggag aagatggagc cgctgcgcat cgcccagggc cgatccggcg
    11821 cccagggaat gatcgggcgc gcgccagcct tcaaggccct gctggcgctg attgcccgcg
    11881 tcggcccgtc gcaggccagc gtgctgctgc tgggcgaatc gggcaccggc aaggagctgg
    11941 ccgcgcgcgc cgtgcaccag gccagcgcgc gcgccgcacg ccccctggtg gtggtggact
    12001 gcgccagcct gcccgagacc ctgttcgaaa gcgagctgtt tggccacgag cgcggcgcct
    12061 tcaccggcgc ccacgcggcg cgcgccggct tggtcgaggc cgcgcatggc ggcaccctgt
    12121 ttctggacga agtgggcgac atccccctga ccatgcaggt caagctgctg cgcctgctgg
    12181 agagcggcac ctaccgccgc gtgggccaga gcgagttgcg ccgcgccgac atccgcctgg
    12241 tggcggccac gcaccgcgac ctgcaggcca tggtgcgcga ggggcgcttt cgccaggacc
    12301 tgtactaccg cctgagcacc ttccccatac gcctgccggc gctgcgcgag cgtgtgcagg
    12361 acattgcgct gctggccgag tcgctgctgg cgcgcgtggc gccgggccgg cagctggcgc
    12421 tggcgcccgc ggccctgcgc cgcctgatgc agcacccctt tgccggcaac gtgcgcgagc
    12481 tgcgcaacgt gctggagcgc gccgccttgc tgaccgacgg cagcacgatc acgctgcaga
    12541 cggtggagca ggcactggcc ttcgacgtgc agccggacac atcggtggcc acgcctgcgg
    12601 gcacgccggc caccgggcgc gccacgctgc gcagcatgga agacgacatc ctgcgcgccg
    12661 ccctgcgggc cccggccggc acacgccagg aacaggcccg cgccctgggc atcagcccgc
    12721 gcacgctgta ccgcaagctg cggcagctgc aggaaggcga aaccaggttc agtcagtagg
    12781 cggcggcagc gccagccccg cctggcgcac cacgcgcacg cagtcctgaa tatgctgctg
    12841 ccccagaaag cggatcgcgc cgtagccaat ggcggccgac gccgcctgcc ccaccagcgg
    12901 cacaaagcgc gccacctgca ccgtggtcag gcgcacgccg atcagcctgg ccgagcgcag
    12961 cagcaaatcc tgcgtgatga acttgcccac cagcagcgcg cccacctggg cgatggcctt
    13021 ttgcagcttg tcgcgctcgc gcggatccag ccggtcgatc tgctgcgggg tcaggccaaa
    13081 gtggtgattg atggccggaa tcaaacgcga cagcagggcc gcgtccaccg cccagtccag
    13141 ccccggcacc ggcagggcac tggccgcgcc ggccaccagc gcgcgccgat gcagcaggcg
    13201 ctggctgcgc tctatggccg ccagcaggcc cgggtcgccc tgggccaggc gccgtgccga
    13261 cagcatggcg ccggccctac ttgagcacct tggtggcgac gagcagcagc acgaacgcac
    13321 ccaccaccga ggcgatgaaa cccgccgccg cccccgccgg gtacaggccc aggaactggc
    13381 ccgcataggt ggcgatgaac gaccccgcca cacccagcag cgaggtcatg atcagtccca
    13441 gggcctgctt gccgggcagc agaaaacgcg caacccagcc cacgatcagg ccgatgaaaa
    13501 gggtgccaag gaggttcagc atgggagacc ttctgtcaag aagaatgaac gggaacggca
    13561 atgtaatgct acgaacgggg cgccaatgtc aaccattgcc agcgggaggc ctggttgcct
    13621 gggcaacaaa ggttgatcgt catcccggcg acggcagagt ccagcgacac
//
LOCUS       DAOMTQ010000080        13652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_760_length_13652_cov_27.107524, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000080 DAOMTQ010000000
VERSION     DAOMTQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_760_length_13652_cov_27.107524"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /gene="pcnB"
                     /locus_tag="PK518_09110"
     CDS             complement(<1..1537)
                     /gene="pcnB"
                     /locus_tag="PK518_09110"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRM49114.1"
                     /translation="MIKTFIDKLLGKSPSGRGAKSRFGKRQEVPAAVHGIDPGLVDRR
                     ASDVVRTLKDAGFEAYIVGGAVRDLLLGLKPKDFDVATNATPEQVKGLFRRAFIIGKR
                     FRIVHVVYGRGREHEVIEVSTFRAYLDNAAAEAVSGNEKTSKHALAGMQHAVDASGRV
                     LRDNVWGPQDEDATRRDFTVNAMYYDPETQVVVDFHKGIDDAKKRVLRMIGDPATRYR
                     EDPVRIIRAVRFAAKLSALGFALEPKTAKPLVACEPLLQEVPQSRLFDEMLKLLQTGH
                     ALASIAQLQKLGLEKGIYPLLDVVVERASSTFVQAALQDTDRRVGEAKTVAPSFLLAC
                     VLWQDVKAGWEQRLTRGEHAFPALQDAIDEVFDKRIGDVSGRGKLAADMREIWVMQPR
                     FEKRSGNTPFSMIAHIRFRAGFDFLRLRADVGEVEEGLAEWWQQFSLADDARREDMIE
                     QVREEQRARQRKAQPVVRRVPKAAEPATVPAAEGPAGVDGADGDAAAPKKRRRRRRKP
                     AGGG"
     gene            complement(1542..2237)
                     /locus_tag="PK518_09115"
     CDS             complement(1542..2237)
                     /locus_tag="PK518_09115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM49115.1"
                     /translation="MPSDQSAPRPRLALFDLDHTLLPLDSDYEWGEFTTRIGWTDREE
                     FGRRNQAFYDDYVAGRLDVHDYVRFATEAVRLRGAAAAAEAHARFMREVIAPAIHAQA
                     RALLQKHHDAGDEVLIITATNEFVTRPIAQALGVPQLLATELARDADGWITGEILGVP
                     CMREGKVQRMQQWLAGRGLSWQDVDSTFYSDSMNDVPLLEKVNHPVATNPDARLRALA
                     QERGWRVLDLFHA"
     gene            complement(2265..2957)
                     /gene="hda"
                     /locus_tag="PK518_09120"
     CDS             complement(2265..2957)
                     /gene="hda"
                     /locus_tag="PK518_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HRM49116.1"
                     /translation="MKQLALDIGLATGPTLDNFHAGPNAQALQHLRLAVGDGAAPRSP
                     VPSYLWGDAGTGKTHLLKATYDALRSQGASVGWLDPSVAVPTAFDERWSAVLLDDVQL
                     YSTAQQAAAFNWFVNAINPTAGVPRWVLAAGNLPPADLPLRDDLRTRLAWGHVFQLQL
                     LDEAERRAVLRQQADERGIVLADEVMDYMLSRFSRDLGSLAQLLERLDGFALRTQRAV
                     TIPLLKTMLETE"
     gene            3327..5279
                     /locus_tag="PK518_09125"
     CDS             3327..5279
                     /locus_tag="PK518_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRM49117.1"
                     /translation="MHKKTLWVMAALPTAVLLASCGGGDGDRLIVDNDPNSCTVRSSG
                     GAAVVVGSGVSGDPAAPEKASGYRLGLTAKHSSKYMAVANTPLATKAGCDVLKAGGSA
                     VDAAIAMQAVLGLVEPQSSTIAGSAFMVYYDAKTKKVTAYDGRETAPAAATNYYLVRQ
                     DQGDTTSPTPVPSARRSGRSIGVPGVMRMLDLAHKEHGRLAWNGLFDYGIQLSTNGFQ
                     IPGRLGAAIASNASSLALDANAMATYFHADGTPRITGETMTNLPYARTLKALAAQGAD
                     ALHTGDIAKAIVAKAGQTVGDDPAKTPITPSLMTLSDLANYKAKKREAVCTNYRNTYH
                     VCTMSPPSSGGIAIAQSLGILSQFDLAQYAPSNPANEGGVPSVMGVHLVSEAERLAYA
                     DRDKYVADTDFVPLPAQGLASLLSPDYLKQRASLISLDKSMGTAAPGDFGPVPQGVDK
                     TPENGTTHLSVVDAYGNAVSMTTTVESSMGSFHMVEGFLLTNQLTDFSANPVDSNGLP
                     VANRIAPGKRPRSTMAPTLVFKGNAPGDFVMATGSPGGGAIIQYVLKTVVGALDWGLD
                     AQQATSLVNFGAANSPTTGVDGSNTTLDLTALVNELKAKGHTVSSNAQSSGVATIMRV
                     TKDGRAQLQGGADPRREGIVLGDGSL"
     gene            5421..6470
                     /gene="purM"
                     /locus_tag="PK518_09130"
     CDS             5421..6470
                     /gene="purM"
                     /locus_tag="PK518_09130"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRM49118.1"
                     /translation="MSSSTPSTPISYKDAGVDIDAGDALVERIKPLARKTMREGVMAG
                     IGGFGALFEVPKRYQEPVLVSGTDGVGTKLKLAFEWNMHDTVGIDLVAMSVNDVLVQG
                     AEPLFFLDYFACGKLDVDTAAAVVGGIARGCELSGCALIGGETAEMPGMYPAGEYDLA
                     GFAVGAVEKSKILNGQNVTAGDMVLGLASSGVHSNGFSLVRKCIERASANGTIPEQLD
                     GKPFKAAIMEPTRLYVKNVLAALAQHPIKALSHITGGGLLENIPRVLPDGLAAHLKAG
                     SWPQTELFAWLQKTAGIDDLEMNRTFNNGIGMVVVVPSDAAEATAATLRALGETVYQI
                     GAIAERGAGAAVVVA"
     gene            6490..7575
                     /locus_tag="PK518_09135"
     CDS             6490..7575
                     /locus_tag="PK518_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM49119.1"
                     /translation="MSSGPVPQGAPEPLPTSKSAAAPVARGVAIASFVLMGAALLLVM
                     LQGLLPGLLCVCLGFLLTRWLAPRLVRLRRLGPGGRGYSHSGQSVAAAVVMLSPLLLI
                     AFALSHSRTYIVDAPQQYRELLDYLASTVLELRQKLPPDIADQLPAGTAEVQRVIASY
                     LAAKAGTLAMAGRAWLTGLLYAYVGLLIGALAAVRSLGAARGPLTQHLVLRVARMGEA
                     FRQIVAAQFWIAAFNTLFTAVFLLLVLPLWDLRLPYTPALITLTFLAGLVPIVGNLLC
                     NAVITLVGLSVSPIAAAACLAFLILIHKAEYVINAKVVGRRTHIGVWELLCVMFVAEA
                     VFGPAGLVAAPLFYAYLKKELAAARLI"
     gene            7703..8098
                     /locus_tag="PK518_09140"
     CDS             7703..8098
                     /locus_tag="PK518_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM49120.1"
                     /translation="MTILVAYVPRPEGRAALDKGIEIATRRNERLVVINAGPGGRQDD
                     PSIVNGYEVERVEERLTALPITAEFRQFVRGKSAIDEIEELVSALQVSVLVIGLRKRS
                     PVGKLLLGSMAQEILLNVECPVLAVKATA"
     gene            complement(8150..8998)
                     /locus_tag="PK518_09145"
     CDS             complement(8150..8998)
                     /locus_tag="PK518_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM49121.1"
                     /translation="MPSNYSVPTPLEYFAALVQVGQGDAIALLEAAACIAHDTYPDLD
                     VQQFLDEMDRLQERLQRRMPQDAQPLQRLNVLNRFFYGELGFSANLNDYYAPDNSYLH
                     VVLSTRRGIPISLALLWLELAQAVGLKAYGVSFPGHFLVKVLLPGGQAVLDPIFGHAL
                     SREALSERLEPFRQHQDAVQEEVPLGLYLQAASARDMLARMLRNLKEIHRTRLDWQHF
                     VAVQDRLITLLPDAWEEWRDRGLAQAELGRPLQAAQDLEVYLAHAQGAGDVRQIQQRI
                     LELRRP"
     gene            complement(9024..10589)
                     /gene="murJ"
                     /locus_tag="PK518_09150"
     CDS             complement(9024..10589)
                     /gene="murJ"
                     /locus_tag="PK518_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRM49122.1"
                     /translation="MSLFKAASTVSLLTLASRVTGLVRDLLMASIFGANVLTDAFNVA
                     FRIPNLFRRLFAEGAFSQAFVPVLAAHRTQHGEEATHQLIDAVATALFWVLLLTCALG
                     VVGAPLLVWALASGLHQDAAGFEAAIFMTRWMFPYIGFMSLVALSAGVLNTWKRFAVP
                     AATPVLLNLCMIAAAWLGAPQLAARGIEPIYAMVGGVMLGGVLQLAVQLPVLYRLGLL
                     PRIGMSWASVRSAWQNPGVRRILTLMAPALLGVGVAQISLMINTQIASYLTPGSVTWL
                     FYADRLMEFPTALLGVALGVVLTPQLSAARAAGDEERYSGMLDWGLRLVVLLAVPCAV
                     GLLTFATPLVATLFHHGALQASDVGQIAVALAGYGVGLLGLVAIKVLAPGYYASQDIR
                     TPVRIAIVVLVFTQLMNAALVPLLAHAGLALSIGLGAIINALWLLSGLLRRGSFRPRP
                     GWGRLGLQVVAASALLAVFLAWGSLHFDWLALRAHSLERAGLLALLLGVAIVLYFGAL
                     WACGLKPREFLRR"
     gene            10695..10994
                     /gene="rpsT"
                     /locus_tag="PK518_09155"
     CDS             10695..10994
                     /gene="rpsT"
                     /locus_tag="PK518_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRM49123.1"
                     /translation="MASTKPKKKNPRLASGRKRARQGLKLNAANTSLRSKYRTAVKNV
                     EKAVLAGDKTKATELFAKAQSVLDTIADKGIFHKNKAARDKSRLSAKVKALALAA"
     gene            complement(11117..11431)
                     /locus_tag="PK518_09160"
     CDS             complement(11117..11431)
                     /locus_tag="PK518_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HRM49124.1"
                     /translation="MVAPTSKEVFIQGITHEGRTFRPSDWAERLAGVMSQFRPGGARP
                     GSHLSYSPWCIPTTMGGVKCVVVNRELQDFEPMAWDFVLNFAKDNNLQIVEACLLPDP
                     PR"
     gene            11661..12857
                     /locus_tag="PK518_09165"
     CDS             11661..12857
                     /locus_tag="PK518_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRM49125.1"
                     /translation="MTAFIEAASPHVMNTYGRVPIALERGQGVRVWDVNGKQYLDGLG
                     GIAVNTLGHNHPRLVPALQEQIAKLIHTSNYYHVPGQEDLAQLLTARAHMTNVFFCNT
                     GLEANEAAIKIARKYGVDKGIEKPVIVVYDHAFHGRSIATMTATGNPKVRNGFGPLLD
                     GFIRVAPNDIEALQEATEGNANIVAVLMEPIQGEGGLHPMRAEYMQQVRQLCDANGWL
                     LMLDEVQAGMGRTGKWFAHQWAGIVPDVMTLAKGLGSGVPVGAVLAHGAAAEVLKPGN
                     HGSTFGGNPLAMRAGVETIRIMEEDRLLDNATAVGAHLKAALQQALGGMAGVNEVRGQ
                     GLIIGVELDRPCGALIDRAADAGLLLSVTAERVIRLVPPLILTTAEADEIVALLKPLV
                     QAFLAE"
     gene            12868..>13652
                     /gene="argF"
                     /locus_tag="PK518_09170"
     CDS             12868..>13652
                     /gene="argF"
                     /locus_tag="PK518_09170"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRM49126.1"
                     /translation="MKHYLQFADFSADEYQYLFSRAAIIKGKFKSYEKYHPLADRTLA
                     MIFEKASTRTRVSFEAGMYQLGGSVVHLTTGDSQLGRAEPIEDSARVISRMTDLVMIR
                     TFGQDKIARFAEYSRVPVINGLTNEFHPCQILADIFTFIEHRGSIAGKVVAWVGDGNN
                     MANTWLQAADLLGFTVHVSTPGGYEVDEKLAFGARQPRADCYKVFSNPMDACRGADLV
                     TTDVWTSMGYEAENEARKKAFADWCVDAEMMAVAKSDALFMHCL"
BASE COUNT         2426 a         4670 c         4420 g         2136 t
ORIGIN      
        1 cgccgccccc ggcgggcttg cggcggcgcc ggcggcgctt cttgggcgct gcggcgtcgc
       61 catcagcgcc gtccacgccg gccgggcctt cggccgcagg cacggtggcc ggctcggccg
      121 ccttgggcac gcgccgcacc accggctggg ccttgcgctg gcgcgcgcgc tgctcctcgc
      181 gcacctgctc gatcatgtcc tcgcggcgcg catcgtcggc cagcgagaac tgctgccacc
      241 actcggccag gccttcctcg acctcgccga catcggcgcg cagacgcagg aagtcaaagc
      301 ccgcgcgaaa gcggatatgg gcaatcatgc tgaatggcgt attgcccgaa cgcttttcaa
      361 agcgcggctg catgacccag atctcgcgca tgtccgccgc cagcttgccg cgcccggaga
      421 catcgccgat gcgcttgtcg aacacctcgt cgatggcgtc ctgcagcgcc gggaaggcgt
      481 gctcgccgcg tgtcaggcgc tgctcccagc cggccttgac gtcctgccac agcacgcagg
      541 ccagcaaaaa gctgggcgcc acggtcttgg cctcgccgac gcggcggtcg gtgtcctgca
      601 gggccgcctg cacgaaggtg ctggacgcgc gctcgaccac cacgtccagc agcgggtaaa
      661 tgcccttctc cagccccagc ttctgcagct gtgcgatgga agccagcgca tggccggtct
      721 gcaagagctt gagcatttcg tcgaacagac ggctttgcgg cacctcctgc aggagcggct
      781 cgcaggccac caggggcttg gcggtcttgg gttcgagcgc aaaaccgagg gcgctgagct
      841 tggcggcaaa gcgcacggcg cggatgatgc gcaccgggtc ttcgcggtag cgcgtggccg
      901 ggtcgccaat catgcgcagc acgcgctttt tggcgtcgtc tatgcccttg tggaaatcca
      961 ccaccacctg cgtctcgggg tcgtagtaca tggcgttgac ggtgaagtcg cgccgcgtgg
     1021 cgtcctcgtc ctgcgggccc cagacgttgt cgcgcagcac gcggccgctg gcgtccacgg
     1081 catgctgcat gccggccagg gcgtgcttgc tggttttctc gttgccgctc acggcctcgg
     1141 ccgcggcgtt gtccaggtag gcgcggaagg tggagacctc gatcacctca tgctcgcgcc
     1201 cgcggccgta gaccacatgc acgatgcgaa agcgcttgcc gatgatgaag gcgcgccgga
     1261 acagcccctt gacctgctcg ggcgtggcgt tggtggccac gtcgaagtcc ttgggcttca
     1321 ggcccagcag caggtcgcgc acggcgccgc cgacgatgta ggcctcgaag cccgcgtcct
     1381 tgagcgtgcg caccacgtcg ctggcccgcc tgtccaccag tccgggatca atgccgtgca
     1441 ctgccgcggg cacctcctgg cgcttgccaa agcggctctt ggcgccgcgg cccgaggggc
     1501 tcttgccgag cagtttgtcg atgaaggtct tgatcatttt tttaggcgtg aaacaggtcc
     1561 aggacgcgcc agccgcgctc ctgtgccagc gcgcgcaggc gcgcatcggg gttggtggcg
     1621 acggggtggt tcaccttctc cagcagcggc acgtcgttca tggagtcgct gtaaaacgtg
     1681 ctgtccacgt cctgccacga caggccacgc ccggccagcc actgctgcat gcgctgcacc
     1741 ttgccctcgc gcatgcaggg cacgcccagg atctcgccgg tgatccagcc gtccgcgtcg
     1801 cgcgccagct cggtggccag cagctgcggc acgcccaggg cctgggcgat ggggcgggtg
     1861 acgaattcgt tggtggcggt gatgatgagc acctcgtcgc ccgcatcatg gtgcttttgc
     1921 agcagcgcgc gcgcctgcgc gtggatggcc ggggcaatca cctcgcgcat gaaccgggcg
     1981 tgcgcttcgg cagcggcggc agcgccgcgc aggcgcaccg cctcggtggc aaagcgcaca
     2041 tagtcgtgca cgtccagccg ccctgccacg tagtcgtcgt aaaaggcctg gttgcgccgg
     2101 ccaaactcct cgcgatcggt ccagccgatg cgggtggtga actcgcccca ctcgtagtcc
     2161 gaatcgagtg gcagcagggt gtgatccagg tcgaacagcg ccagccgtgg acggggcgcc
     2221 gattggtcag aaggcatggg ttttccagtg gagccgaagg ccggtcattc ggtctccagc
     2281 atggttttga gcagcggtat ggtcacggcg cgctgggtgc gcagcgcaaa gccgtcaagg
     2341 cgctccagca gctgcgccag gctacccagg tcgcgcgaga agcggctgag catgtagtcc
     2401 atcacttcgt cggccagaac gatgccgcgt tcatcggcct gctgacgcag cacggcgcgg
     2461 cgctcggcct cgtccagcag ctgcagctga aacacatggc cccaggcgag gcgcgtgcgc
     2521 aggtcatcgc gcagcggcag gtcggccggc ggaaggttgc cggcggccag cacccagcgc
     2581 ggcacgccgg ccgtggggtt gatggcgttg acaaaccagt tgaacgccgc cgcctgctgg
     2641 gccgtgctgt agagctgcac gtcgtccagc agcacggcgc tccagcgctc gtcgaatgcg
     2701 gtcggcacgg ccaccgaagg atcgagccag cccacgctgg cgccctggct gcgcagggcg
     2761 tcgtaggtgg ccttgagcaa atgcgtcttg cccgtgcccg catcgcccca cagatagctg
     2821 ggcacgggcg agcgcggcgc ggcgccgtca cccacggcca ggcgcaggtg ctgtagcgcc
     2881 tgggcattcg gcccggcgtg gaaattgtcc agcgtggggc ccgtggccag gccgatgtcg
     2941 agcgccagtt gcttcatggg gctgatgccg gcacctggtc attggcaggt gcggcgaata
     3001 ggggctttgg caaaggaaag ggcatggccc agggacagga aaaacagccc ggccaggcac
     3061 ggccgagacg aaatgcaaaa cgtttgattt tagtcggtcg ccatgtacca cggcggccac
     3121 cccttgtgcc agccggcgcg ccatggggac agaggtaacc aggtgctacc gacacaagaa
     3181 ttcaagggtt tgcaccagcc gccatccggg ggcgcgtcac taagcttttc agcctcttcc
     3241 cgagaaaccc ccaaaccccc gagcatccgg cccaggcgcc cgtgacatgg cgcccagacc
     3301 ttgctcattc accaagagga tggaacatgc acaagaaaac actctgggtt atggctgcgc
     3361 tgcccacggc ggtgcttctc gccagttgcg gcggtggtga cggcgaccgc ttgatcgtcg
     3421 ataacgaccc caacagctgc accgtgcgca gcagcggggg cgctgcggtg gtcgtgggct
     3481 ccggcgtgag cggcgacccg gcggcgccgg agaaggcctc cggctaccgc ctgggactga
     3541 cggccaagca ttccagcaaa tacatggccg tggccaacac ccccttggcc accaaggccg
     3601 gctgcgacgt gctcaaggcc ggtggctcgg cggtggacgc ggccatcgcc atgcaggccg
     3661 tgctgggcct ggtggagccg cagtcgagca ccatcgccgg cagtgccttc atggtctact
     3721 acgacgcgaa aaccaagaag gtcacggcct acgacgggcg cgagaccgct cccgcggccg
     3781 ccaccaacta ctacctggtg cggcaagacc agggcgacac gacctcgcca acacccgtgc
     3841 caagtgcgcg gcgcagcggc cgttccatcg gcgtgccggg tgtgatgcgc atgctcgacc
     3901 tggcacacaa ggagcatggc aggctggcct ggaacggact gttcgactac ggcatacagc
     3961 tgtccacgaa tggcttccag attcccggcc gcctgggcgc agcgattgcc agcaatgcca
     4021 gcagcctggc gctggatgcc aacgccatgg ccacctactt ccacgccgat ggcacgccgc
     4081 gtatcacggg cgaaaccatg accaacctgc cctacgccag gacgctcaag gccctggcag
     4141 cccagggtgc agatgcactg cacacgggag acatcgccaa ggccatcgtt gccaaggccg
     4201 ggcagacggt gggcgacgac ccggccaaga cacccatcac ccccagcctg atgaccctca
     4261 gcgacctggc caactacaag gccaagaagc gcgaggcggt gtgcaccaac taccgcaaca
     4321 cctaccatgt gtgcaccatg tcgccgccct cgtcgggcgg cattgccatt gcgcagtcgc
     4381 tgggcattct gagccagttc gacctggccc agtacgcgcc cagcaacccg gccaacgaag
     4441 gcggcgtgcc cagcgtgatg ggtgtgcatc tggtctcgga agccgagcgc ctggcctacg
     4501 ccgaccgcga caagtacgtc gccgacaccg acttcgttcc gctgccggcc cagggcctgg
     4561 cctcgctgct cagccccgac tacctcaagc agcgcgccag cctgatcagc ctggacaaga
     4621 gcatgggcac ggccgccccg ggcgacttcg gcccggtgcc gcagggcgtg gacaagacgc
     4681 cggaaaatgg cacgacccac ctgtcggtgg tcgatgccta tggcaacgcg gtgtcgatga
     4741 cgaccaccgt ggaatccagc atgggctcat tccacatggt ggaggggttc ttgctgacca
     4801 accagctgac ggacttctcg gccaatccgg tggacagcaa tggcctgccc gtggccaacc
     4861 gcatcgcccc gggcaagcgc ccgcgcagca ccatggcgcc cacgctggtc ttcaagggca
     4921 acgcgccggg cgacttcgtc atggccacgg gttcaccggg cggcggcgcc atcattcagt
     4981 acgtgctgaa aaccgtggtc ggcgcgcttg actgggggct ggacgcgcag caggccacct
     5041 cgctggtcaa ctttggcgcg gccaacagcc cgaccaccgg cgtggacggc tccaacacca
     5101 cgctggatct gacggccttg gtcaacgaac tgaaggccaa ggggcatacg gtcagcagca
     5161 atgcccagtc cagcggcgtg gccaccatca tgcgtgtgac caaggatggc agggcgcaac
     5221 tgcagggcgg cgcagacccc cggcgtgagg gcatcgtgct gggcgacggc tcgctttgaa
     5281 cccctgcccc catgctcctc aagccccgcc catggcgggg cttttttcct gccaaacggg
     5341 cattggccaa gtccaggcgg cgggccttta aaatcactgg tttgtgccgc accggccaaa
     5401 ccccaagtcc cgcaatattc atgagctcct ctaccccctc cacccccatc tcgtacaaag
     5461 acgccggcgt tgacatcgac gcgggcgacg cgctggtcga gcgcatcaag cccctggcca
     5521 ggaagacgat gcgcgagggc gtcatggccg gcatcggcgg cttcggcgcg ctgttcgagg
     5581 tgcccaagcg ctaccaggag ccggtgctgg tctcgggcac cgacggcgtg ggcaccaagc
     5641 tcaaactggc ctttgaatgg aacatgcacg acacggtggg catagacctg gtggccatga
     5701 gcgtcaacga cgtgctggtg cagggcgcgg agccgctgtt cttcctcgac tatttcgcct
     5761 gcggcaagct cgatgtggac acggccgccg ccgtggtggg tggcattgcg agaggctgcg
     5821 agctgtccgg ctgcgccttg atcggcggcg agaccgccga aatgcccggc atgtaccctg
     5881 ccggcgagta cgacctggcc ggctttgccg tgggcgcggt ggagaagtcc aagattctca
     5941 atggccagaa cgtgaccgcc ggcgacatgg tgctgggcct ggcttccagc ggcgtgcact
     6001 ccaacggctt ctccctggtg cgcaagtgca tagagcgcgc cagcgccaat ggcacgatcc
     6061 cggagcagct cgatggcaag cccttcaagg ccgccatcat ggagcccacg cgcctgtacg
     6121 tgaagaacgt cctggccgcg ctggcgcaac acccgatcaa ggccctgtcc cacatcaccg
     6181 gcggcggcct gctggagaac attccacgcg tgctgcccga cggcctggcg gcccacctga
     6241 aggccggcag ctggccccag accgagctgt ttgcctggct gcagaagacc gccggcatcg
     6301 atgacctgga gatgaaccgc accttcaaca acggtatcgg catggtcgtg gtcgtgccct
     6361 ctgatgccgc cgaggccacg gccgcaacac tgcgtgcgct gggcgagacg gtgtaccaga
     6421 ttggtgccat tgccgagcgt ggcgcgggcg ccgccgtggt tgttgcctga cccacggcaa
     6481 ccacccaaca tgtcatcggg accggtgccg caaggcgcac ccgaacccct gccgacgtcc
     6541 aagagcgccg ctgcaccagt ggcgcgcggc gtggccatcg ccagctttgt gctgatgggt
     6601 gccgccctgc tgctggtcat gctgcaggga ctgctgcccg ggttgttgtg cgtgtgcctg
     6661 ggctttttgc tcacgcgctg gctggcgccg cgactggtgc ggctgcgccg gctgggcccg
     6721 ggcgggcgcg gctattccca cagcggccaa agcgtggccg cggcggtggt gatgctgtca
     6781 ccgctgctgc tgatcgcttt cgcactgtcg cactcgcgca cctacatcgt tgatgcaccc
     6841 cagcagtacc gtgagttgct ggactatctg gcaagcaccg ttctggagct gcgccagaaa
     6901 cttccgcccg atatcgcaga ccaattgccc gccggcacgg ccgaggtgca gcgcgtcatc
     6961 gcctcctacc tggcggccaa ggccggcacc ctggccatgg ccggacgagc ctggctcacg
     7021 ggcctgctgt atgcctatgt cggcctgctc ataggcgcgc tggcggccgt gcgcagcctg
     7081 ggcgcggcgc gcggcccgct gacgcagcat ctggtgctgc gcgtggcccg catgggcgag
     7141 gcgtttcgcc agatcgtggc ggcgcagttc tggattgccg ccttcaatac cctgttcacg
     7201 gccgtgttcc tgttgctggt gctgccgctg tgggatctgc ggttgcccta cacgccggcg
     7261 ctcatcaccc tcacctttct ggccgggctg gtgcccatcg tgggcaacct gctgtgcaac
     7321 gccgtgatca cgctggtggg cctgtcggta tcccccatcg ccgcagctgc ctgcctggca
     7381 ttcctgatcc tgatccacaa ggccgaatac gtgatcaacg ccaaggtcgt gggccggcgc
     7441 acccatatcg gcgtgtggga actgctttgc gtgatgttcg tggccgaggc ggtattcggc
     7501 ccggcagggt tggtggccgc ccccttgttt tatgcctatc tcaagaaaga gctggcagcg
     7561 gcgcgcctga tatgacgacg gcaccgcgat tttttctcat gcaccatgcc tgccctcgtc
     7621 aatgggcagg ccttgggcta ttctgctgtc tggcgtcaaa gcgcccgaca ttttttcaac
     7681 tcaccacaag ggagacaccc gcatgaccat tctcgtcgcc tacgttcctc gcccagaagg
     7741 ccgggccgcg ttggacaagg ggatagaaat cgcgacccgc cgcaatgagc gcctggtcgt
     7801 catcaacgcc ggacctggcg ggcgccagga cgatcccagc atcgtcaacg gctatgaagt
     7861 cgaacgcgtc gaggaacgcc tcacagcgct gcccatcacc gccgagtttc gccagttcgt
     7921 gcgcggcaag agcgccatcg atgaaatcga ggaactggtc tcggcgctgc aggtgtcggt
     7981 gctcgtgatc ggcctgcgca agcgctcgcc cgtgggcaag ctgctgctgg gcagcatggc
     8041 gcaggaaatc ctgctcaacg tcgaatgccc ggtgctggcc gtcaaggcca cagcctgacg
     8101 agcgccgccc tcgcctgcgc gcccgccacc atggcgggcg ttgtcgtttt cagggcctgc
     8161 gcagctccag gatgcgctgc tgtatctgcc gcacatcgcc cgcgccctgg gcgtgcgcca
     8221 gatagacctc cagatcctgc gccgcctgca gcggccgccc cagttccgcc tgtgccaggc
     8281 cgcggtcgcg ccactcttcc caggcgtcgg gcagcagggt gatcagcctg tcctgcacgg
     8341 ccacgaaatg ctgccagtcc aggcgcgtgc ggtgaatttc cttgaggttg cgcagcatgc
     8401 gcgccagcat gtcgcgcgcg ctggcggcct gcaggtacag acccaggggc acctcctcct
     8461 ggaccgcgtc ctgatgctgg cgaaaaggct ccaggcgctc gctcagcgcc tcgcgcgaaa
     8521 gagcgtggcc aaaaatcgga tcaagcaccg cctgcccgcc cggcaacagc accttgacca
     8581 gaaaatgccc ggggaaggac accccatacg ccttcagccc cacagcctgt gccagctcca
     8641 gccacaacaa ggccagcgat atcggaatac cacgccgggt gctcaacacc acatgcaggt
     8701 agctgttgtc gggcgcgtag tagtcgttca ggttggcgct aaaccccagc tcaccataga
     8761 aaaagcgatt cagaacattg agccgctgca gcggctgcgc gtcttgcggc atgcggcgct
     8821 gcaggcgctc ctgcagcctg tccatctcgt ccagaaactg ctgcacgtcc aggtctggat
     8881 aggtgtcatg ggcaatgcag gccgcagcct ccagcagggc aatggcatca ccctgcccca
     8941 cttgcaccag tgcagcaaaa tattccagcg gtgtgggtac agagtagttc gatggcatgg
     9001 gctgctcgcc aatatccgca ggcctaccgg cgcaggaatt cgcgcggctt cagcccgcag
     9061 gcccacagcg caccaaaata aagcacgatc gcgacgccca gcagcagcgc cagcaagcct
     9121 gcacgctcca ggctgtgagc gcgcagcgcc agccagtcga aatgcagcga gccccaggcc
     9181 agaaacaccg ccagcaacgc actcgccgcc accacctgca gccccaggcg cccccagccc
     9241 ggccggggac ggaagctgcc gcggcgcagc aggcccgaga gcagccacag cgcattgatg
     9301 atggccccca ggccgatcga caaggccagg ccggcatgcg ccaacagcgg caccagtgcg
     9361 gcattcatga gctgcgtgaa caccagcacc acgatggcga tgcgcacggg cgtgcgaatg
     9421 tcctggctgg cgtagtagcc cggcgccagc accttgatgg ccaccaggcc cagcaggccc
     9481 acgccatagc cggccagggc cacggcgatc tggcccacgt cgctggcctg cagggcgcca
     9541 tggtggaaca gcgtggccac cagcggcgtg gcaaaggtca gcaggcccac ggcgcagggc
     9601 acggccagca gcaccaccag gcgcaggccc cagtcgagca tgccggaata gcgctcctcg
     9661 tcgcccgcgg ccctggccgc cgacagctgc ggcgtgagca ccacgccgag ggccacgccc
     9721 agcagggccg tcggaaactc catcagccga tcggcataga acagccaggt cacgctgccc
     9781 ggtgtgagat aggacgcgat ctgggtgttg atcatcagcg agatctgcgc cacccccacg
     9841 cccagcaagg ccggcgccat cagcgtgagg atgcggcgca cgcccgggtt ctgccaggcg
     9901 ctgcgcacgc tggcccagct catccctatg cgcggcagca ggcccaggcg gtacagcacc
     9961 ggcagctgca ccgcaagctg cagcacgccc cccagcatca caccgccgac catggcgtaa
    10021 atgggctcga tgccacgcgc cgccagctgc ggcgcaccca gccaggcggc ggcaatcatg
    10081 cacaggttca gcagcacggg tgtggcggcc ggcacggcaa agcgtttcca ggtgttgagc
    10141 acgcccgccg acagcgccac cagcgacatg aagccgatgt agggaaacat ccagcgcgtc
    10201 atgaagatcg cggcctcgaa acccgccgca tcctggtgca gcccgctcgc cagggcccac
    10261 accagcagcg gcgcgcccac cacgcccagt gcgcaggtca acagcaacac ccagaacagg
    10321 gccgtggcca cggcgtcgat cagctggtgt gtggcttcct cgccatgctg cgtcctgtgc
    10381 gccgccagca cgggcacaaa ggcctggctg aaagcccctt cggcaaacag gcggcgaaac
    10441 aggttgggga tgcggaatgc gacgttgaac gcgtcggtca acacattcgc cccgaagatc
    10501 gaggccatga gcaggtcgcg caccagcccc gtcacgcgcg aggccagggt cagcagggat
    10561 acggtggaag cggctttgaa cagtgacacc ggcgcaagtg tatgcgtttg acgccaagct
    10621 ggccgcgaag caacgcgcgg gctataatcg ccggctttgc tgacaacatc ctcagacact
    10681 aaggaataca taccatggca tctaccaagc caaagaaaaa gaacccccgc ctggcatcgg
    10741 gccgcaagcg cgcccgccaa ggcctgaagc tcaacgcagc caacacctcg ctgcgctcca
    10801 aataccgcac tgccgtcaag aacgtcgaga aggccgtcct ggccggcgac aagaccaagg
    10861 ccaccgagct gttcgccaag gcccagtccg tgctcgacac cattgccgac aagggcatct
    10921 tccacaagaa caaggccgct cgcgacaaga gccgcctgtc cgccaaggtc aaggccttgg
    10981 cactggccgc ctgaccctct caggcaaaca gcccgggtga ttgcagtcac ccgccgtttg
    11041 taacgggtgc gctgtcagca aagcaaaaaa ccgcccaagg gcggtttttt tgtgtctgga
    11101 gcatggcgcc ccgcagtcaa cgcggcggat cgggcagcag gcaagcctcg acaatctgca
    11161 ggttgttgtc tttggcaaag ttgagcacaa agtcccaggc catgggctcg aagtcctgta
    11221 actcgcgatt caccaccacg catttcacgc cgcccatcgt cgtcggaatg caccaggggg
    11281 aatagctcag gtggctgccg ggccgggcgc caccagggcg gaactggctc atcactcccg
    11341 ccagccgctc ggcccagtcg ctgggtcgga aggttcgccc ttcatgggta atgccttgga
    11401 tgaagacttc tttggaggtg ggagcaacca tcgggttccg gtcgtaataa aggccgctgg
    11461 cctggtatgt cagcgtggcg ccgattctaa ctcttatata agagctaacc ctaataacag
    11521 ccgctgccca ccctgcatgg ttgatgtacg catgactgcg atgcgcattc ttcaatgatg
    11581 cgcttggggc cagcgcctag aatcgcgctt cccttttgtc ggcccacaag ggccatgacc
    11641 gtaccgcagg agaagtccgc atgaccgcat ttatcgaggc tgcctcgccc cacgtgatga
    11701 acacctatgg ccgcgtgccc atcgcactcg agcgcggcca gggtgtgcgc gtgtgggacg
    11761 tgaacggcaa gcagtacctc gacggcctgg gcggcatcgc ggtgaacacg ctggggcaca
    11821 accacccccg tctggtgccg gcgctgcagg agcagatcgc caaactgatc cacacgtcga
    11881 actactacca tgtgcctgga caggaagatc tggcccagct gctgacggcg cgcgcacaca
    11941 tgaccaacgt gttcttctgt aacaccggcc tggaggccaa tgaggccgcc atcaagatcg
    12001 cgcgcaagta cggcgtggac aaggggatag agaagcccgt gatcgtggtc tacgaccacg
    12061 ccttccacgg ccgctccatc gccaccatga cggccacggg caaccccaag gtgaggaacg
    12121 gcttcggccc gctgctggac ggcttcatcc gcgtggcgcc caacgacatc gaggcgctgc
    12181 aggaggccac cgagggcaac gccaacatcg ttgccgtgct gatggagccc atccagggcg
    12241 aaggcggcct gcaccccatg cgcgccgaat acatgcagca ggtgcgccag ctgtgcgacg
    12301 ccaacggctg gctcttgatg ctcgacgagg tgcaggccgg catgggccgc accggcaagt
    12361 ggtttgccca ccagtgggcc ggcatcgttc ctgacgtgat gacgctggcc aagggcctgg
    12421 gctcgggcgt gccggtgggc gcggtgctgg cgcatggcgc ggcggccgag gtgctcaagc
    12481 ccggcaacca tggctccacc ttcggcggca acccgctcgc catgcgcgcc ggcgtggaga
    12541 cgatccgcat catggaagaa gaccggctgc tggacaacgc caccgccgtg ggcgcgcacc
    12601 tgaaggccgc gctgcagcag gcgctgggcg ggatggccgg tgtgaacgag gtgcgcggcc
    12661 agggcctgat catcggcgtg gagctggacc gcccctgcgg ggcgctcata gaccgcgcgg
    12721 cggacgccgg cctgctgctg tcggtgacgg ccgagcgcgt gatccgcctg gtgccgccgc
    12781 tgatcctcac gaccgccgag gccgacgaga tcgtcgccct gctcaagccc ctggtgcaag
    12841 ccttcctggc cgaatgaaag ccccgttatg aagcactacc tgcaatttgc cgatttcagc
    12901 gccgacgagt accagtacct gttctcgcgc gccgccatca tcaagggcaa gttcaagagc
    12961 tacgagaagt accacccgct ggccgaccgc accctggcca tgatcttcga gaaggccagc
    13021 acgcgcaccc gcgtctcgtt cgaggccggc atgtaccagc tgggcggctc ggtggtgcac
    13081 ctgaccacgg gcgacagcca gctcgggcgc gccgagccca tcgaggacag cgcccgcgtc
    13141 atcagccgca tgaccgacct ggtgatgatc cgcaccttcg gccaggacaa gatcgcgcgc
    13201 tttgccgaat attcgcgcgt gcccgtcatc aacggcctga ccaacgaatt ccacccctgc
    13261 cagatcctgg ccgacatctt caccttcatc gagcaccgcg gctccattgc cggcaaggtg
    13321 gtggcctggg tgggcgacgg caacaacatg gccaacacct ggctgcaggc ggccgacctg
    13381 ctgggcttca ccgtccatgt gagcaccccc ggcggctatg aagtagatga aaaattggcc
    13441 ttcggcgccc gccagccgcg cgcagactgc tacaaagttt ttagcaaccc gatggacgcc
    13501 tgccgcggcg ccgacctggt caccaccgac gtctggacca gcatgggcta cgaggccgag
    13561 aacgaggcgc gcaagaaggc ctttgccgac tggtgcgtgg acgccgagat gatggccgtg
    13621 gccaagagtg acgccctgtt catgcactgc ct
//
LOCUS       DAOMTQ010000081        13622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_768_length_13622_cov_26.283482, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000081 DAOMTQ010000000
VERSION     DAOMTQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_768_length_13622_cov_26.283482"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PK518_09175"
     CDS             complement(<1..116)
                     /locus_tag="PK518_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM49127.1"
                     /translation="MKVAVIGAGWGGMAAAVQAAQAGHAVTVFEAGRAPGGRA"
     gene            complement(113..955)
                     /gene="hpnD"
                     /locus_tag="PK518_09180"
     CDS             complement(113..955)
                     /gene="hpnD"
                     /locus_tag="PK518_09180"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HRM49128.1"
                     /translation="MSTPEQYAQEKAAASGSSFYYAFLFLPKERRAAITAFYAFCREV
                     DDVVDEVSDPGVAQAKLAWWQDEVAKAFAGQPSHPVMQALMPHVPAHGLRQDQLQAVI
                     EGCQMDLEQARYADFRSLQRYCHLVAGVVGEVAAQIFGQQDAHTTQYAHKLGLAFQLT
                     NIIRDVGEDAMRGRIYLPVSELQQFEVTANEISNRTYSDRFTALMRFQAERAHLFYDQ
                     ALALLPEVDRRTQKPGLMMASIYRTLLREIEADGFQVLHQRTSLTPLRKFWLAWKMQA
                     LGLM"
     gene            complement(1005..1931)
                     /locus_tag="PK518_09185"
     CDS             complement(1005..1931)
                     /locus_tag="PK518_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRM49129.1"
                     /translation="MAGSTPSPALLWRMTRPAFLLLTVVACLLGTATAAACGCGLDWW
                     LAAAATLLAVLAHAAGNLLNDLHDAKSGADAANHQGIYPFTGGSRLIQRGLVTEGQTA
                     ALAHTLLLVLVPAGLLLAVKTAGGVLLLGLAGLLLVWAYSAPPLRLMARGLGEPVVAG
                     AWFLVVLGSDYVQRRQFFIIPASAALSFALLVAALLLINGCPDADADARVGKHTLAVR
                     LGPLGAAWAYLGLVLAAHGWLAASVWLLIPPATALWGLVSLPLSLAAASLLLQRAHQP
                     QRLKPALALTVAATLLHGVGMAAGFALVALQH"
     gene            complement(1932..2819)
                     /gene="hpnC"
                     /locus_tag="PK518_09190"
     CDS             complement(1932..2819)
                     /gene="hpnC"
                     /locus_tag="PK518_09190"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HRM49130.1"
                     /translation="MPAVNPSPSSPDTLAPAMAAPVTHYENFPVASLLCPPGLRQPVA
                     AIYAFARTADDIADEGEATAGERLSDLAAYRAELRAAAAGQAPGARWAGVFGPLQVAL
                     QSHRLPTALLKDLLDAFAQDVAMTEAGACYPDREELLDYCRRSANPVGRLLLHLYGVQ
                     DDAALRESDAICSALQLINFWQDLSVDLPRGRHYLTDADCAAHGVARADLAARQRSAA
                     SDRLILDCAAWARALMLQGAPLVHRLPGRAGWELRLVVQGGLRILDKVDALQGASLCQ
                     RPVLRPLDWLRMAARALAM"
     gene            2911..4104
                     /locus_tag="PK518_09195"
     CDS             2911..4104
                     /locus_tag="PK518_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM49131.1"
                     /translation="MPSFPALTRPRSGLFLCSLTACVALLTACSRHEPAPEPVRSVKL
                     VTVGPSALQMQLEYAGEVRARIESRIGFRVAGKIVQRQAELGQRVQAGQLLARLDVSD
                     YALATQAARAQVAAATTQRDLAAADFERFSKLKAQNFISGAELDRREAQLKSAQAQLD
                     QARAQLATQGHQADYTRLLADAAGVVTGIEAEPGQVVAAGTPVVRIARDGPRDAVFFV
                     PEDRVGQLRVGLPVQVRAWSADTLMPGEVREVAASADPVTRTFLVKVAVQAKDVPPLG
                     ATVYVTPQWHAAEGLQAIKLPTSSLRQEGGETAVWVYEPGDGGGGGGTVRSQVVQVAT
                     ADGNEAVIAAGLAPGMQVVASGVHVLTPGQKVTVYKPKFDQTPADKAPAAINNDKAAA
                     PAAAR"
     gene            4114..7251
                     /locus_tag="PK518_09200"
     CDS             4114..7251
                     /locus_tag="PK518_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM49132.1"
                     /translation="MALLEPKKGFNLSRWALEHVPLTRYLMVVLMLLGFAAYFQLGQD
                     EDPPFTFRAMVIHTNWPGATAQQVAEQVTDKIERTLQEVPYADKIRSYSKPGESQVIF
                     ELKDSSKPAEVANTWYQVRKKIGDMRHTLPGNIQGPFFNDEFGDVYGVIYALRGEGFG
                     YAELKDFADDTRQRLLRVKDVAKVEQFGVQPEKIWIEVSQKRLAQLGLDQAGVLQQLG
                     SQNAVESAGTIQTPQDMVQVRVAGQFTSVEQLRAMPIRAPQSQAQLRLGDIAEVHRGY
                     VDPPDVKVRFQGQEVIALGISMTKGGDIIALGKALRAAVADIQQRLPAGVELAQVQDQ
                     PAAVAASVNDFVKVLIEAVVIVLAVSFVSLGLHKGGRFGWYVDWRPGLVVAITIPLVL
                     AVTFLAMHYFGIGLHKVSLGSLIIALGLMVDDAIIAVEMMVRKMEEGYDKMRAATFAY
                     DITAMPMLTGTLITAAGFLPIGIAKSVTGEYTFAIFAVTVIALVLSWIVSVYFVPYLG
                     TLLLRVKPHDPDAPPHEVFDTPFYARFRRTVDWCVRRRWTTLAITLAIFALGLVGMGR
                     VQQQFFPDSSRPEVMVELWYPEGTSFAANEEVTLRVEQRLLQQAGVKNVATWVGSGTP
                     RFYLPLDQVFPQSNVSQFIVQAASLGEREQLRRSLPALLATEFPEARGRVKLLPNGPP
                     VPYPVQFRVIGSDPAVLRQRADEVKTVMRANTHMIGVNDNWNESVKAVRLEIDQEKAR
                     ALGVSSQSIAQAARTMFTGTTVGQYREGDRLIDIVLRQTQDERDTISGIANAYLPTAS
                     GRSIPLTQIARPVFSWEPGVMWRSGRDYAITVQGDLVEGMQGATITEQLLPELRTLEA
                     RWQATGGNGVRIEVAGAVEESSKGSSSIVAGVPIMLFIVFTLLMLQLHSFSRSMLVFL
                     TGPLGIAGVAGALLLLGRPFGFVALLGVIALMGMIQRNSVILIDQIELNRAAGRSTWD
                     SIVTAAVWRMRPIALTAAAAVLAMIPLSRNVFWGPMAVAIMGGLIVATVLTLLALPAM
                     YAAWFRVKKPDSEPE"
     gene            7357..7443
                     /locus_tag="PK518_09205"
     tRNA            7357..7443
                     /locus_tag="PK518_09205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7391..7393,aa:Leu,seq:tag)
     gene            7514..8827
                     /gene="tig"
                     /locus_tag="PK518_09210"
     CDS             7514..8827
                     /gene="tig"
                     /locus_tag="PK518_09210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRM49133.1"
                     /translation="MAVTVETLEKLERKITLSLPVTTIQSEVDARLKRLARTVKMDGF
                     RPGKVPMSVVAQRYGYSVQYEVLNDKVGEAFAQAANEANLRVAGQPRITEKDGAPEGQ
                     VSFDAVFEVFPEVKIGDLSEAEVEKVSAEVTEVAIDKTVDILRKQRRSFAQRAQGAPA
                     EDGDRVTVDFEGKIDGEPFQGGKAEDFQFLVGEGQMLKEFEDAVRGMKSGESKTFPLA
                     FPEDYHGKDVAGKTADFLVTVKKIEAAHLPEVNEQLAKSLGIADGTVEGLRADIKKNL
                     EREVKFRLLARNKAAVMDALVSKAELDLPNASVQAEVARLLESARADLKQRGIKDADK
                     AEIPDDIFRPQAERRVRLGLVVAELVRANELQAKPEQLKAHVDELAASYEKPEDVIRW
                     YFSDRQRLAEVEAVVVENNVTDFVLGKAKVSEKAVSFDELMGQQA"
     gene            8950..9558
                     /gene="clpP"
                     /locus_tag="PK518_09215"
     CDS             8950..9558
                     /gene="clpP"
                     /locus_tag="PK518_09215"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRM49134.1"
                     /translation="MSALETRALGMIPMVIEQSGRGERSYDIYSRLLKERVIFLVGEV
                     NDQTANLVVAQLLFLESENPDKDISFYINSPGGSVTAGMAIYDTMQFIKPDVSTLCCG
                     FAASMGAFLLAAGAKGKRFSLPNSKIMIHQVLGGARGQATDIEIHARDILRTKDQMNR
                     ILAERTGQPIEKVKADTERDYFMTADEAMDYGIVDQVISKRP"
     gene            9659..10924
                     /gene="clpX"
                     /locus_tag="PK518_09220"
     CDS             9659..10924
                     /gene="clpX"
                     /locus_tag="PK518_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRM49135.1"
                     /translation="MADKKGSSSEKTLYCSFCGKSQHEVKKLIAGPSVFICDECIDLC
                     NEIIRDEQPAGEAAREGRGDLPTPAEIKANLDSYVIGQEKAKRTLAVAVYNHYKRLRH
                     KDKAGKDDVELSKSNILLIGPTGSGKTLLAQTLARQLDVPFVMADATTLTEAGYVGED
                     VENIVQKLLQSCNYDVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLI
                     EGTMASVPPQGGRKHPNQDFLQIDTTNILFICGGAFAGLEKVIENRTEASGIGFGAAV
                     RSKKQRSLTEVYLDIEPEDLIKFGLIPELVGRMPVVTALAELGEDALVQILTEPKNAL
                     VKQYSKLLGMEGVDLEIRPAALKAIARKAIARKTGARGLRSILEQSLIGTMFDLPNVS
                     NVEKVVVDEATIEENKAPLLLYREAVKTA"
     gene            11045..13471
                     /gene="lon"
                     /locus_tag="PK518_09225"
     CDS             11045..13471
                     /gene="lon"
                     /locus_tag="PK518_09225"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRM49136.1"
                     /translation="MSGHTPLPATPLDLPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     TAMDADRRIMLVAQKAAAKDEPQVSDMFEVGCVSTILQMLKLPDGTVKVLVEGQQRAL
                     VDAVHEAESHFTATTTPVPGEVESEDDQSSEIEALRRAVMQQFDQYVKLNKKIPPEIL
                     TSIASIDDPGRLADTIAAHLPLKLENKQRVLDLADVKLRLENLFEQLEREVDILNVDK
                     RIRGRVKRQMEKSQRDFYLNEQVKAIQKELGEGEDGADIDEIEKKIKAAKMPAEARKK
                     AEGELKKLKLMSPMSAEATVVRNYIDVLTSMPWSKKTKIKHDLAYAEDVLNEDHYGLD
                     RVKDRILEYLAVQQRVDKVKAPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGMR
                     DEAEIRGHRRTYIGAMPGKVLQSLNKVGARNPLFLLDEIDKLGTDFRGDPSSALLEVL
                     DPEQNHKFGDHYVEVDFDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKTNIA
                     IKYLLPKQMTNNGVKDEELRITESAVRDIVRYYTREAGVRSLERELSKICRKTVKGLQ
                     LKKLQPQVEVSADNLSEYLGVRKFSYGHAEKQNQVGLVAGLAWTEVGGDLLTIEVATM
                     PGKGNITRTGSLGDVMKESIEAARTVVRSRAHLWGIRDEVFDKRDLHMHVPDGATPKD
                     GPSAGIAMTTAIVSAFTGIPVRADVAMTGEITLRGEVTEIGGLKEKLLAALRGGIKTV
                     LIPEANVKDLQEIPDNVKSGLEIVPVRWIDKVLEVALERKPAALTDHEVTPAVAVAPG
                     GEVVQGSVKH"
BASE COUNT         2511 a         4188 c         4619 g         2304 t
ORIGIN      
        1 gcgcgcccgc ccggcgcgcg gccggcctcg aagacggtga cagcgtggcc ggcctgcgcc
       61 gcctgcacgg cggcggccat gccgccccag ccggcgccga tgacggcaac cttcacatca
      121 gacccagtgc ctgcatcttc caggccagcc agaacttgcg cagcggtgtc aggctggtgc
      181 gctggtgcag cacctgaaag ccgtcggcct cgatctcgcg cagcagcgtg cggtagatgc
      241 tggccatcat caggccgggc ttttgcgtgc gccggtccac ctcgggcagc agggccaggg
      301 cctggtcgta gaacaggtgg gcgcgctcgg cctgaaagcg catcagggcg gtgaaacggt
      361 cggaatatgt gcggttgctg atttcattgg ccgtgacctc gaactgctgc agttcgctca
      421 ccggcaggta gatgcgcccg cgcatggcgt cctcgcccac gtcgcggatg atgttggtga
      481 gctggaaggc caggcccagc ttgtgcgcat actgcgtggt gtgcgcgtct tgctggccga
      541 agatctgcgc ggccacctcg cccaccacgc cggccaccag gtggcagtag cgctgcaggc
      601 tgcgaaagtc ggcgtagcgc gcctgctcca gatccatctg gcagccttcg atgaccgcct
      661 gcagctgatc ctggcgcagg ccatgtgcgg gcacatgcgg catcagtgcc tgcatcacgg
      721 ggtggctggg ctggccggcg aaggctttgg ccacctcgtc ctgccaccag gccagcttgg
      781 cctgggccac gccggggtcg ctgacttcgt ccaccacatc gtccacctcg cggcaaaagg
      841 cataaaaggc ggtgatggcc gcgcgccgtt ccttgggcag gaacaggaag gcgtaataaa
      901 agctgctgcc cgatgcggca gccttttcct gtgcgtattg ttctggggta ctcatcgcgg
      961 ggcgggggtg gctgggcggc gattgtccca tgtgcgccac gggctcaatg ctgcagcgcg
     1021 accagggcaa aacccgcggc catgcccaca ccatgcagca gggtggccgc cacggtgagg
     1081 gccagcgccg gtttcaggcg ctggggctga tgcgcccgct gcagcagcag gctggctgcg
     1141 gccagcgaca ggggcaggga caccagcccc cacagcgccg tggctggcgg tatcagcagc
     1201 cacacgctgg ccgccagcca gccgtgcgcg gccagcacca ggcccaggta ggcccaggcc
     1261 gcgcccagcg gccccagacg cacggccagg gtgtgcttgc ccacgcgcgc gtcggcatcg
     1321 gcgtcggggc agccgttgat gagcagcagc gccgccacca gcagcgcaaa actcagcgcc
     1381 gcgctggccg ggatgatgaa gaactggcgc cgctgcacat agtcgctgcc cagcaccacc
     1441 agaaaccagg cgcccgcgac caccggctcg cccagcccgc gcgccatcag gcgcagcggc
     1501 ggggccgaat aggcccacac cagcagcagc ccggccaggc ccagcagtag cacgccgccg
     1561 gcggtcttca cggccagcag caggccggcg ggtaccagca ccagcagcag ggtgtgggcc
     1621 agggccgcgg tctggccttc ggtgaccagg ccgcgctgga tcagccgcga gccgccggtg
     1681 aaggggtaga tgccctggtg gttggcggcg tcggcgccgc ttttggcatc gtgcaggtcg
     1741 ttgagcaggt tgccggctgc atgggccagc accgccagca gcgtggcggc ggccgccagc
     1801 caccagtcca ggccacagcc acaggcggcg gccgtggccg tgcccagcag gcaggcgacc
     1861 acggtgagca gcaggaacgc cgggcgcgtc atgcgccaca gcagggccgg ggagggtgtg
     1921 ctgcccgcca tctacatcgc cagcgcgcgc gccgccatgc gcagccagtc cagcggccgc
     1981 agcaccgggc gctggcacag gctggcaccc tgcagcgcgt ccaccttgtc caggatgcgc
     2041 aggccgcctt gcaccaccag gcgcagctcc cagccggctc gccccggcag acgatgcacc
     2101 aggggggcac cctgcagcat cagggcgcgg gcccaggcgg cgcagtccag gatcaggcgg
     2161 tcgctggcgg ccgagcgttg gcgcgccgcc aggtcggcgc gcgccacgcc gtgggcggcg
     2221 cagtcggcgt cggtcaggta gtggcgcccg cgtggcaggt ccacactcag gtcctgccag
     2281 aaattgatga gctgcagggc gctgcaaatg gcgtcgcttt cgcgcaacgc agcgtcgtcc
     2341 tgcacgccgt acaggtgcag cagcaatcgc cccacgggat tggccgagcg ccggcagtag
     2401 tccagcagct cctcgcggtc ggggtagcag gcgccggcct cggtcatggc cacgtcctgg
     2461 gcaaaggcgt ccagcaggtc cttgagcagc gccgtgggca gccggtggct ttgcagcgcg
     2521 acctgcagcg gcccgaacac gccggcccag cgtgcgccgg gtgcctggcc cgcggctgcc
     2581 gcgcgcagtt cggcgcggta ggcggccagg tcggacaggc gttcgccggc cgtggcctcg
     2641 ccttcgtcgg caatgtcatc ggcggtgcgc gcaaaggcgt agatggcggc aaccggctgg
     2701 cgcagccccg gcgggcacag cagcgaggcg acgggaaaat tttcgtaatg cgtgacgggc
     2761 gcagccatgg ctggcgcgag ggtgtcgggt gatgaggggc tggggttcac ggccggcatt
     2821 gtcgcttgac agtgaaaatg ggtttcacat agattaccaa ccaattacta accgatcagt
     2881 cattaagcgc cgttgggcgc gcttcgttcc atgccaagct ttcctgccct gacgcggccc
     2941 cgttcggggc tttttttgtg cagtctgacc gcctgcgtgg cgctgctgac ggcctgctcg
     3001 cgccatgagc ctgcgcccga gccggtgcgt tccgtcaagc tcgtgaccgt cgggccgtct
     3061 gcgctgcaga tgcagctgga gtatgcgggc gaggtgcgcg cgcgcatcga gtcgcgcatc
     3121 ggttttcgcg tcgccggcaa gatcgtgcag cgccaggccg agctgggcca gcgtgtgcag
     3181 gccggccagt tgctggcgcg cctggatgtg agcgactatg ccctggccac ccaggccgcg
     3241 cgcgcccagg tggcggcggc gaccacgcag cgcgatctgg cggcggcgga ctttgagcgc
     3301 ttctccaagc tcaaggcgca gaacttcatc agcggcgccg agctggatcg gcgcgaagcg
     3361 caactcaagt cggcccaggc gcagcttgat caggcgcgcg cgcaactggc cacgcagggc
     3421 caccaggccg actacacgcg cctgctggcc gatgccgccg gcgtggtgac gggcatagag
     3481 gccgagcccg gccaggtggt ggcggccggt acgccggtgg tgcgcattgc gcgtgacggc
     3541 ccgcgcgatg cggtgttctt cgtgcccgag gatagggtgg gccagctgcg cgtgggcctg
     3601 ccggtgcagg tgcgtgcctg gtcggccgat acgctgatgc cgggcgaggt gcgcgaggtg
     3661 gcggccagcg ccgacccggt gacgcgcacc ttcctggtca aggtagcggt gcaggccaag
     3721 gatgtgccgc cgctgggggc cacggtctat gtcacgccgc aatggcatgc ggccgagggc
     3781 ctgcaggcca tcaagctgcc cacgagcagc ctgcgccagg agggcggtga gacggcggtg
     3841 tgggtgtacg agccgggcga cggcggcggc ggcggcggta cggtgcgctc gcaggtggtg
     3901 caggtggcca cggccgacgg caacgaggcc gtgatcgccg ccggcctggc gcccggcatg
     3961 caggtggtgg cctcgggggt gcatgtgctc acgcctgggc aaaaggtcac ggtatacaag
     4021 ccaaaattcg accaaacgcc cgcagataaa gcgccggcag ctatcaataa tgataaagct
     4081 gcggcgcctg ccgcggcgcg ttgagggcag gccatggcgt tgctggaacc caagaagggc
     4141 ttcaacctct cgcgctgggc gctggaacat gtgccgctga cgcgctacct gatggtggtg
     4201 ctgatgctgc tgggctttgc cgcgtacttc cagctggggc aggacgagga tccgccgttc
     4261 accttccgcg ccatggtgat ccacaccaac tggcccggcg ccacggcgca gcaggtggcc
     4321 gagcaggtca ccgacaagat cgagcgcacg ctgcaggagg tgccctacgc cgacaagatc
     4381 cgcagctaca gcaagccggg cgagtcgcag gtgatcttcg agctcaagga ttcctccaag
     4441 ccggccgagg tcgcaaacac ctggtaccag gtgcgcaaga agatcggtga catgcgccat
     4501 accctgccgg gcaacatcca ggggccgttc ttcaacgacg agtttggcga cgtgtacggc
     4561 gtgatctacg cgttgcgcgg cgagggcttc ggctacgccg aattgaagga cttcgccgac
     4621 gacacgcgtc agcggctgct gcgcgtgaag gacgtggcca aggtcgagca gttcggcgtg
     4681 cagcccgaga agatctggat cgaggtctcg caaaagcgcc tggcgcagct ggggctggat
     4741 caggccggcg tgctgcagca gctgggcagc cagaacgcgg tggagagcgc cggcaccata
     4801 cagacgccgc aggacatggt gcaggtgcgc gtggccgggc agttcaccag cgtggagcag
     4861 ctgcgcgcca tgccgattcg cgcaccccag tcccaggccc agctgcgcct gggcgacatt
     4921 gccgaggtgc accgcggcta tgtcgatccg cccgacgtca aggtgcgctt ccagggccag
     4981 gaggtgatcg cgctgggcat ctccatgacc aagggcggcg acatcatcgc cctgggcaag
     5041 gcgctgcgcg ccgccgtggc cgacatccag cagcgcctgc ccgccggcgt ggagctggcg
     5101 caggtgcaag accagccggc ggccgtggcg gcgtcggtga acgactttgt caaggtgctg
     5161 atcgaggccg tggtcatcgt gctggcggtg agctttgtgt cgctggggct gcacaagggc
     5221 gggcgctttg gctggtacgt ggactggcgg ccgggcctgg tggtggccat caccatcccg
     5281 ctggtgctgg ccgtcacctt tctggccatg cattacttcg gcatagggct gcacaaggtg
     5341 tcgctgggct cgctcatcat tgccctgggg ctgatggtgg acgacgccat cattgccgtg
     5401 gagatgatgg tgcgcaagat ggaggagggc tacgacaaga tgcgtgccgc cacctttgcc
     5461 tacgacatca cggccatgcc catgctgacc ggcacgctga tcacggcggc cggctttttg
     5521 ccgatcggca tcgccaagtc ggtgaccggc gaatacacct tcgccatctt tgccgtgacg
     5581 gtgatcgcgc tggtgctgtc gtggatcgtc tcggtgtact tcgtgcctta cctgggcacg
     5641 ctgctgctgc gggtcaagcc gcacgaccct gatgcgcctc cgcacgaggt cttcgacacg
     5701 ccgttctatg cgcgttttcg ccgtacggtg gactggtgcg tgcgccgtcg ctggaccacg
     5761 ttggccatca ccctggccat ttttgcgctg gggctggtgg gcatggggcg ggtgcagcag
     5821 cagttcttcc ctgattcgag ccgacccgag gtgatggtgg agctgtggta ccccgagggc
     5881 acctcgtttg ccgccaacga ggaggtgacg ctgcgcgtgg agcagcgcct gttgcagcag
     5941 gccggggtga agaacgtggc cacctgggtc ggttcgggca cgccgcgctt ttatctgccg
     6001 ctggaccagg tgtttccgca gagcaatgtg tcgcagttca tcgtgcaggc cgccagcctg
     6061 ggcgagcgcg agcagttgcg ccgcagcctg ccggcgctgc tggccaccga gtttccggag
     6121 gcgcgcggcc gcgtcaagct gctgcccaat ggcccgcccg tgccctatcc ggtgcagttt
     6181 cgcgtgatcg gcagcgaccc ggccgtattg cgccagcgtg ccgacgaggt caagaccgtg
     6241 atgcgcgcca acacccacat gatcggcgtg aacgacaact ggaacgagtc ggtgaaggcc
     6301 gtgcgcctgg agatcgacca ggagaaggcc cgcgccctgg gggtgagcag ccagtccatc
     6361 gcccaggcgg cacgcaccat gttcaccggc accacggtcg gccagtaccg tgagggtgat
     6421 cgcctgatcg acatcgtgct gcgccagacc caggacgagc gcgacaccat ctccggcatc
     6481 gccaacgcct atctgcccac ggcctcgggg cgctccattc cgctcacgca gatcgcccgg
     6541 ccggtgttca gctgggaacc tggcgtgatg tggcgctccg gccgcgacta cgccatcacc
     6601 gtgcagggcg acctggtgga aggcatgcag ggtgccacca tcaccgagca gttgctgccc
     6661 gagctgcgca cgctcgaggc gcgctggcag gccaccggcg gcaacggcgt gcgtatcgag
     6721 gtcgcgggcg cggtggagga gagcagcaag ggctcgtcct ccatcgtggc tggcgtaccc
     6781 atcatgctgt tcatcgtgtt cacgctgctc atgctgcagc tgcacagctt cagccgctcc
     6841 atgctggtct ttctgaccgg gccactgggc attgccggtg tggccggggc gctgctgttg
     6901 ctgggccggc cgttcggctt cgtggcgctg ctgggggtga ttgcgctcat gggcatgatc
     6961 cagcgcaatt ccgtgatcct gattgaccag atcgagctca accgcgccgc tggccgttcc
     7021 acctgggact ccatcgtgac cgccgccgtc tggcgcatgc gcccgatcgc gctgacagcc
     7081 gccgccgccg tgctggccat gattcccctg tcgcgcaacg tgttctgggg cccgatggcc
     7141 gtggccatca tggggggctt gatcgtcgcc acggtgctca cgctgctggc cttgccggcc
     7201 atgtacgcgg cctggttccg ggtgaaaaaa ccggattcgg agcccgaatg agaccgccga
     7261 aacagaatcg ggtttgcgac aaggtaaaat ccaacgtttg acctatttca ccggggtggc
     7321 cttgaaccag gggccatccg ggtggcttga agaaccgcgc gggtggcgaa attggtagac
     7381 gcaccaggtt taggtcctga cgccagcaat ggtgtggggg ttcgagtccc cccccgcgca
     7441 ccacgtgaga ccttgatgca gcgcagcgcc gtcaaggtcg gcacgatcca catccctgac
     7501 actaggaacc atcatggccg taacggttga aactcttgaa aagctcgagc gcaagatcac
     7561 gctgagcctg cctgtcacca ccatccagag cgaagtcgat gcgcgcttga agcgcctggc
     7621 ccgcaccgtg aagatggacg gcttccgtcc cggcaaggtg ccgatgagcg tcgtggccca
     7681 gcgctacggc tactcggtgc agtacgaggt gctgaacgac aaggtcggtg aagcctttgc
     7741 ccaggccgcc aacgaggcca acctgcgcgt ggccggtcag ccgcgcatta ccgaaaagga
     7801 tggcgccccc gagggccagg tcagctttga cgccgtgttc gaggtattcc ccgaggtcaa
     7861 gatcggcgac ctgtccgagg cagaggtgga aaaggtctcc gccgaagtga ccgaagtcgc
     7921 tatcgacaag accgtggaca ttctgcgcaa gcagcgccgc agctttgccc agcgcgccca
     7981 gggtgcgccc gccgaggatg gcgaccgcgt gaccgtggac tttgagggca agattgacgg
     8041 cgaacccttc cagggcggca aggccgagga cttccagttc ctggtcggcg aaggccagat
     8101 gctcaaggaa ttcgaagacg ccgtgcgcgg catgaagtct ggcgaaagca aaaccttccc
     8161 gctggccttc cctgaggact atcacggcaa ggacgtggcc ggcaagacgg ccgacttcct
     8221 ggtcaccgtg aagaagatag aggccgccca cctgcccgaa gtgaacgagc agctggcgaa
     8281 gtcgctaggt attgccgacg gcaccgtcga aggcctgcgc gccgacatca agaaaaacct
     8341 ggagcgtgaa gtcaagttcc gcctgctggc gcgcaacaag gcggcagtga tggacgccct
     8401 ggtgtccaag gccgagctgg atttgcccaa cgccagcgtc caggccgagg ttgcccgcct
     8461 gctggaatcc gcccgcgccg acctgaagca gcgcggcatc aaggacgccg acaaggccga
     8521 gatccccgac gacatcttcc gtccccaggc cgagcgccgc gtgcgcctgg gcctggtggt
     8581 agccgagctg gtgcgcgcca acgagctgca ggccaagccc gagcagctca aggcccatgt
     8641 ggacgagctg gctgccagct acgaaaagcc ggaagacgtg attcgctggt acttcagcga
     8701 ccgccaacgc ctggccgagg tggaggccgt ggtcgttgaa aacaatgtca ccgatttcgt
     8761 cctgggcaag gccaaggtca gcgaaaaggc ggtgtcgttc gacgaactga tgggccagca
     8821 ggcctgatac acgcaggtgg ttcgcctgat ggaggcgcgc caccggtgaa gctttgcggg
     8881 gcttgtgctt tgcggcacag gccccatgtt atttttctga atacggtatc cgcctgactg
     8941 gagacataca tgagcgcatt ggaaacacgg gccctgggca tgattcccat ggtcatcgag
     9001 cagtcgggcc gcggcgagcg gtcttacgac atttattcac gcctgctcaa ggagcgcgtg
     9061 attttcctgg tgggtgaggt gaatgaccag accgccaacc tggtggtggc gcagctgctg
     9121 ttcctcgaga gcgagaaccc ggacaaggac atttcgttct atatcaactc ccccggtggc
     9181 agtgtcacgg ctggtatggc gatctacgac accatgcagt tcatcaagcc ggacgtgtcc
     9241 acgctgtgct gcggctttgc ggccagcatg ggagcgtttt tactggcggc cggtgccaag
     9301 ggcaagaggt tttccctgcc caactcgaag atcatgatcc atcaggtgct gggcggcgcg
     9361 cgcgggcagg cgaccgacat cgagatccac gcacgggata tcctgcgcac caaggatcag
     9421 atgaaccgca tcctcgccga gcgcactgga caacccattg agaaggtcaa ggcggacacc
     9481 gagcgcgact atttcatgac cgctgacgaa gctatggact atggcatcgt tgatcaggtg
     9541 atctccaagc gcccctgagg gccttatttg cccggatgcg gcgtttcccc ctgcgggaag
     9601 cgccgttttt atttcgttat catcttcccg acttccccag tgataaggca ttgcttccat
     9661 ggccgataaa aaaggctcct ccagcgagaa aaccctttac tgctcttttt gcggcaagag
     9721 ccagcatgaa gtaaagaagc tgatcgccgg accctccgtc tttatttgcg acgagtgcat
     9781 tgacctgtgc aacgaaatca tccgtgacga acaacccgcg ggtgaagctg cgcgcgaggg
     9841 tcggggcgat ctgcccacgc ccgccgagat caaggccaac ctggacagct atgtgattgg
     9901 ccaggaaaag gccaagcgta ccctggccgt ggccgtgtac aaccactaca agcgcctgcg
     9961 ccacaaggac aaggccggca aggatgatgt cgagttgtcc aagagcaata tcctgctcat
    10021 cggccccacc ggctccggca agacgctatt ggctcagacc ctggcgcgtc agcttgatgt
    10081 tcctttcgtg atggccgatg ccaccacgtt gaccgaggcc ggctatgtgg gcgaggacgt
    10141 ggagaacatc gtccagaagt tgctgcaaag ctgcaactac gacgtcgagc gcgcgcagcg
    10201 cggtattgtc tacatcgacg aaatcgacaa gatcagccgc aagagtgaca acccgagcat
    10261 cacgcgcgac gtgtcgggcg agggcgtgca gcaggccttg ctgaaattga tcgaaggcac
    10321 catggccagc gtgccgcctc agggtggacg caagcatccg aaccaggact ttctgcagat
    10381 cgacaccacc aacatcctgt ttatctgcgg tggcgcgttc gcggggctgg aaaaggtcat
    10441 cgaaaaccgt accgaggctt cgggcattgg ttttggtgcg gccgtgcgca gcaagaagca
    10501 gcgttcgctg accgaggtgt acctggatat cgagcccgag gatttgatca agttcggtct
    10561 cattcccgaa ctcgtggggc gcatgccggt ggtgaccgcc ctggccgaac tgggtgagga
    10621 tgcgctggtg caaatcctga ccgagcccaa gaatgcgctg gtcaagcagt acagcaagct
    10681 gctgggcatg gagggagtcg atctggagat acgcccggcc gcactcaaag ccattgcccg
    10741 caaggccatt gcccgcaaga cgggcgcgcg ggggctgcgc tccatcctgg agcaatccct
    10801 gatcggcacc atgttcgacc tgcccaacgt ttccaacgtg gaaaaggtgg tggtggacga
    10861 ggccaccatc gaagagaaca aggcgcctct gttgctctac cgcgaggcgg tcaagacagc
    10921 ctgaggctcg ccagtttgtc ccgctgcggc gaccactggc gccatgcgcg cctgacagtg
    10981 gtgtcgccgg gttgaaaaac gctgtccatg gaccatatgc catggactta actgaggaat
    11041 gcccatgtct ggacatacgc ccctgcccgc tacacccctg gatctgccat tgctgccgct
    11101 gcgcgatgtg gtggtattcc cccatatggt gatccccctg ttcgtgggcc gtcccaaaag
    11161 catcaaggcc ctggagacgg ccatggatgc cgatcgcagg atcatgctcg tggcccaaaa
    11221 ggcggccgct aaagatgagc cgcaggtgtc cgacatgttt gaagtcggct gcgtttctac
    11281 catcctgcaa atgctcaagc tgcccgatgg cacggtgaag gtgctggtcg aaggccagca
    11341 gcgcgccctg gtggatgcag tgcatgaggc cgagtcacac ttcactgcca cgacaacgcc
    11401 cgtgccgggc gaggtcgagt ccgaggatga ccagtccagc gagatcgagg cgctgcgccg
    11461 tgcggtgatg cagcagtttg accagtacgt caagctgaac aagaagattc cgcccgagat
    11521 tcttacttcg atcgccagca tcgacgatcc tggccgtttg gccgacacca ttgcggcaca
    11581 tctgccgttg aagctggaaa acaagcagcg tgtgctcgat ctggcggatg tgaagctgcg
    11641 tctggaaaac ctctttgagc agctggagcg cgaggttgac atactgaacg tggacaagcg
    11701 catccggggc cgtgtgaagc gccagatgga aaagagccag cgcgacttct atctgaacga
    11761 gcaggtcaag gccattcaga aggaactcgg agagggcgag gatggcgcag acatcgatga
    11821 gatcgagaaa aagatcaagg ccgccaagat gcccgccgag gcacgcaaga aggccgaggg
    11881 tgagttgaaa aaactcaagc tgatgtctcc catgtcggcc gaggctacgg tggtgcgcaa
    11941 ctacatcgat gtgcttacca gcatgccctg gagcaagaag accaagatca agcacgatct
    12001 cgcgtacgct gaagatgtat tgaacgaaga ccactacggg ttggatcgag tgaaggatcg
    12061 catcctggaa taccttgctg tgcagcagcg cgtggacaag gtgaaggcgc ccatcctgtg
    12121 cctggtgggg ccaccggggg tgggcaagac ctcgctgggc cagtccattg ccaaggcgac
    12181 tgggcgcaaa tacgtgcgta tggcgctggg aggcatgcgt gacgaggccg aaattcgtgg
    12241 tcaccgccgc acctacatcg gcgccatgcc gggcaaggta ttgcaaagcc tgaacaaggt
    12301 tggcgcgcgc aacccactgt ttctgctgga tgaaatcgac aagctgggta cggatttccg
    12361 aggggatccg tcgagcgccc tgctggaggt gctcgaccct gagcagaacc acaagttcgg
    12421 tgatcactat gtcgaggttg attttgacct gtcagatgtc atgttcgttg ctaccagcaa
    12481 ctcgatgaac atcccgccag ctctgctcga tcgcatggag gtgattcgcc tatcgggtta
    12541 taccgaggac gagaagacca acatcgctat caaatacctg ctgcccaagc agatgacgaa
    12601 caacggcgtc aaggatgagg agttgcgaat caccgagtcg gctgtgcgcg acatagtgcg
    12661 ttactacacg cgcgaggctg gcgtgcgctc gctggagcgt gaactgtcca agatctgccg
    12721 gaagacggtc aagggcttgc agctcaagaa gttgcagcca caggtcgagg tgtcggccga
    12781 caacttgtcc gaatacctgg gggtgcgcaa gttcagctat gggcatgcag agaagcagaa
    12841 ccaggtgggt ttggtggccg gcctggcatg gaccgaggtg ggaggtgatt tgctgaccat
    12901 cgaggtggcc accatgcctg gcaagggcaa catcacgcgc acgggctcgc ttggtgatgt
    12961 gatgaaggaa tctattgagg ccgcacgcac ggtggtgcgc agccgagcgc atttgtgggg
    13021 tattcgggac gaggtgtttg acaaacgcga tttgcatatg catgtgcctg acggcgctac
    13081 gcccaaggac ggacccagcg ctggtattgc catgaccacg gccatcgtct ctgcattcac
    13141 tggcataccg gtgcgtgccg atgtcgccat gaccggtgag atcacattgc gcggcgaagt
    13201 caccgagatc ggtggcttga aggaaaagct gctggcagcc ctgcgcggag ggatcaagac
    13261 agtgctgatc cctgaagcaa acgtgaaaga cttgcaggag atccctgaca acgtgaagag
    13321 tggcctggag atcgtgcccg tgaggtggat agacaaggtg cttgaggtag cgctggagcg
    13381 caagccagct gccctgacag atcacgaagt aacgcctgcg gttgctgtgg cgcccggcgg
    13441 tgaggtggtg cagggctctg tgaagcactg atgaaaaaag tcggcttttt tcttgtggga
    13501 ctcaaaaagt agactacaat ttaccccatc gatgcgaaac gccatataat ggcaggcttc
    13561 ggtaaaatgc gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgtcg
    13621 gt
//
LOCUS       DAOMTQ010000082        13487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_780_length_13487_cov_25.745086, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000082 DAOMTQ010000000
VERSION     DAOMTQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13487
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_780_length_13487_cov_25.745086"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="PK518_09230"
     CDS             <1..1906
                     /locus_tag="PK518_09230"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRM49137.1"
                     /translation="RAALAGPIWAGTPPAARADALARAADLLEQRSQPLMGLIQREAG
                     KTLPNAVAEIREAVDFLRYYGAQVAAEFDNASQRPLGVVLAISPWNFPLAIFAGQVAA
                     ALAAGNTVLAKPAEQTPLTAAAMVQILHEAGVPQDALQLVPGRGESVGAALVAHPQVA
                     GVMFTGSTEVARIIARQLAKRLSPSGQAIPLIAETGGQNAMIVDSSALAEQVVGDVLA
                     SAFDSAGQRCSALRVLCLQEDVAERTLHMLRHALQEWNMGNPDQLSTDVGPVIDAEAR
                     AQIEAHIERMQAAGQKVTRVQRGNGAALNGHFVAPAIIEIDSPARLTREVFGPVLHVI
                     RFQREQLDQLIDGINATGYGLTFGMHSRIDETIAHLTERVHAGNIYVNRNVIGAVVGV
                     QPFGGMGLSGTGPKAGGPLYLHRLVQGNPNAALASVPTAEAAPASRALLERLTQANLP
                     GASATQLRALIDAALAATRVGASWLLPGPTGESNRYRLLPRGAVWALPASTLGLVHQV
                     VAALASGNPCHVVLPEQDSGCTALWQALRQSAGAEGTAWLHSAEAASLDDAATPMAAL
                     LFEGDGDALLQVAARVAARDGAIVRVESRSSDELATGRSYDLAALVHEQSISTNTAAA
                     GGNAQLMTMA"
     gene            2056..3576
                     /gene="putP"
                     /locus_tag="PK518_09235"
     CDS             2056..3576
                     /gene="putP"
                     /locus_tag="PK518_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HRM49138.1"
                     /translation="MQYNWNDPTAIMFAVYLVAMLGIGALGYLGTKNLSDYILGGRSL
                     GSFVTALSAGASDMSGWLLMGLPGAVYLSGLSESWIAIGLVAGAYLNWRLVAARLRLY
                     TERAGNALTLPDYLANRFEDKGNLLRIVTALVILVFFTLYCASGVVAGARLFENMFGM
                     PYATALWVGAACTIAYVLIGGFLAVSWTDTIQASMMITALILAPVMAYLAVEGQLQPG
                     QDWSAIVSAEKFDLFKGASFVGIVSLLAWGLGYFGQPHILVRFMAASSVQTIPAARRI
                     SMTWMILCLMGAVAVGFVGIPFFAAHTSGAAAVNANAETVFMEVSKQLFNPWIAGALL
                     AAILAAVMSTLSCQLLVCSSALTEDIYRVFLRKNASQTELVWVGRIMVLVVALIAIAI
                     ASNPEAKVLGMVSYAWAGFGAAFGPVIILSLFWGRMTRGGALAGIIVGAVTVLVWKQF
                     ALWGLYEIVPGFILGWVSVVVASLLSAEPSKSMRQAHATVEREFAQISQGRWVSAT"
     gene            3700..4503
                     /locus_tag="PK518_09240"
     CDS             3700..4503
                     /locus_tag="PK518_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM49139.1"
                     /translation="MKKTPTDVQPKPSVQVLERMFQLIDVLASREESVSLKEISERTG
                     LHPSTAHRILNDMTIGRFVDRPESGSYRLGMRLLELGNLVKARLSVRDAAMAPMRHLH
                     KLIQQPVNLSVRQGDEIVYVERAYSERSGMQVVRAIGGHAPLHLTSTGKLFLASEDTQ
                     RVRAYATRTGLPGHTRNSITQLPMLERELSKARQYGIARDNEELELGVRCMAAGVFDD
                     QGQLVAGLSISAPADRLDDGWVPKLQAAAQEISLALGYSTGQRTVLQGV"
     gene            complement(4552..5637)
                     /locus_tag="PK518_09245"
     CDS             complement(4552..5637)
                     /locus_tag="PK518_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRM49140.1"
                     /translation="MHARFRIATRFSQVLAIAALGLSLAFPGAQAAERKPAASKKQSQ
                     VVAKRAAVKSSAKAQRKAGSVKSTRMAARSAKAPVRMAAEPARLSFGQLAGLHSVSDP
                     LDLKSSVALVIDQDTKEVLLSKNDHAVLPIASLTKLMTGLLVSEARLPMDEPITITQD
                     DVDTEKGSGSRLTVGTTLSRGELLHLALMSSENRAAHALGRTYPGGLTAFVQRMNAKA
                     RLLGMTESRFVEPTGLSSSNQSSAHDLATLVNVAHGDSLLRELSTSPGHEVTVGSRVL
                     QYNNTNRLVKSPTWDIGLQKTGYISEAGRCLVMQAEVAGRKLIMVFLDSAGKFSRLGD
                     AERVRSWVEKMHPGSSISLGAAATPRS"
     gene            complement(5756..7297)
                     /locus_tag="PK518_09250"
     CDS             complement(5756..7297)
                     /locus_tag="PK518_09250"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRM49141.1"
                     /translation="MSSDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLKVDVI
                     EAGFAASSNGDFECVQAIARAIKDSTICSLSRANDRDIARAAEALRDAERGRIHTFIA
                     TSPLHMEKKLRMTPEEVLEQSKLAVRFARNLMGDIEFSAEDGYRSEIDFLARVCEAVI
                     NEGATTINIPDTVGYAIPELYGNFIKTLREKVPNSDKAIWSVHCHNDLGMAVANSLAG
                     VKIGGARQVECTINGLGERAGNCSLEEIVMAVKTRRDYFGLDVGIDTSQIVPASRMVS
                     TTTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWSANKIVLGKLS
                     GRTAFKQRLQELGVVLESEGEIMAAFAKFKDLADRKSEIFDEDILALVGDESVTAEKE
                     QYGFISLAQRSETGEMPHAEIVFTIDGREVRGTSDGNGPVDASLKAIESQVKSGAEMV
                     LYSVTAISGNTESQGEVTVRLQNTGRIVNGVGADPDIVVASAKAYLSALNKLQSKAER
                     VAAQG"
     gene            complement(7328..8995)
                     /gene="leuA"
                     /locus_tag="PK518_09255"
     CDS             complement(7328..8995)
                     /gene="leuA"
                     /locus_tag="PK518_09255"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRM49142.1"
                     /translation="MLHNPASKYRPFAPVRLADRRWPDAVITQAPIWCSVDLRDGNQA
                     LIEPMDIARKMRMFEQLVKIGFKEIEVGFPSASQVEFDFVRKLIEEDRIPDDVTIQVL
                     TQAREPLIRRTFEALAGAPRAIVHLYNATAPVMRRVVLGMSEDEIVELAVSHAQMFQD
                     LAARQPATDWTFEYSPEMYSDTELAFSKRVIDAVTAVWQPTPEKKCIINLPTTVEHST
                     PNIFADMVEWTHRHIARRDSVVLSVHPHNDRGTGTATAELALMAGADRLEGCLFGNGE
                     RTGNLDVVNVALNMYTQGVHPGLDFSDIDAIRSTVEYCNQLPVHPRHPYVGDLVYTSF
                     SGSHQDAIKKAFAERKEGDIWDMPYLPIDPKDLGRSYEAVIRVNSQSGKGGIAYLLES
                     EYGLQLPRRLQIEFSAAVQREMDASGKELTAADLWALFQSEYQIQAVAAPAYRMQDDG
                     GRMQLAATVQWQGDILDLEGEGTGPIDAFVQALSTALDRQLRVLDYTEHAIGEGANAQ
                     AVAYVEMRIDERQTVYGVGMDANIVSASMRAILSGLQRAPQMQVVAA"
     gene            complement(9185..10087)
                     /locus_tag="PK518_09260"
     CDS             complement(9185..10087)
                     /locus_tag="PK518_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49143.1"
                     /translation="MNVTLRQLRAFVAVADTGSFTLAAERLFITQSALSGLIKELEST
                     LGLRLFDRSTRRLRLSDTGRELYPQIEKILHDLDGVVSEVGNLKDLQRGTVRVAVPQL
                     LACTLLPELMAGFRAQYPGVSLRLVDCAVESVMARVFSGEVDIGIGPEREANSDIAAE
                     QLFSLPFMAVMPPTHALARRKKLLWPDLSGQSLITLQGQFTELLVSDVGEAARDMHKE
                     AFMQVTYMTTALALARAGLGITLCMPYARTLVEQFGLVMRPVGHPVVERSFWVFTRRG
                     RALSPAAQEFRTFLEDQLQSSRLC"
     gene            10194..11159
                     /locus_tag="PK518_09265"
     CDS             10194..11159
                     /locus_tag="PK518_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM49144.1"
                     /translation="MTDKKFASQADLEEKKISWEKLSDNAYAYTAEGDPNTGVIIGDD
                     GCMIIDATATPVAAQGVIAKIREITDKPIKYVVLTHYHAVRVLGASGYKTEGLEQIIA
                     SRDTYDLIVERGQQDMDSEIGRFPRLFQAVESVPGLTWPTMTFEGEMALWLGKLEVRI
                     KQVGRGHTKGDAIVYIPSQNICFSGDLVEADAACYTGDAYLADWPQTLDNLAAMKFDK
                     LIPGRGPALMTPADVQKGLAYTRDFVSTLYKSAQEAVAQGMDLNATMKHTRQAMDPKF
                     GHVFIYEHCLPFDVTRAHDEASGIRDPRIWTAERDQQMWHALQQA"
     gene            11238..12902
                     /locus_tag="PK518_09270"
     CDS             11238..12902
                     /locus_tag="PK518_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM49145.1"
                     /translation="MLKTYTYPKFDYVRPPELVEQHSGHYPVVIVGAGPVGLSMAIDL
                     AAQGQKVLLLDNDDTVSIGSRGVCYAKRTLEVLDRMDCGSPCVDKGMSWNVGRTFFGE
                     GEVFNFNLRPQDGHNRPGMVNLQQYYLEDYLVQRARQLPNLEMRFKNNVISVEQDDKQ
                     ATVRVETPDGAYTLTTDWLVAADGARSPIRNMLGLEIDGKIFMDRFLIADVIMKADYP
                     AERWFWFDPPFHPNQSVLLHKQADNVWRIDFQLGWDADPEAEKKPEKVIPRIKAMLGD
                     EREFELEWVSVYTFQCRRMGQFNHNRVLFVGDAAHQVSPFGARGANSGIQDSDNLAWK
                     LKLVIDGKAPASLLDSYSSERCYAADENIMNSTRSTDFITPKSKTSKAFRNAVLELAQ
                     DYPFARALVNSGRLSVPSWLVDSPLNTPDEDGFAGNMVPGAPMDDAPVTGGKTAWLLG
                     DMDTRFHLLHYVEDAGALSAQQAQALAGLADHPIAVHAIVVAQRGTAPAGLATLIDAQ
                     GALRERYDLQPGTSYLLRPDQHVAARWRQLDAAKVRAAVNRATGNV"
     gene            12920..13150
                     /locus_tag="PK518_09275"
     CDS             12920..13150
                     /locus_tag="PK518_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HRM49146.1"
                     /translation="MSLNTQPNLREPGKRYFQAYSPGDDFYEALIDTHQGLSDEQSAA
                     LNARLILLLSNHIGDITVLREALAIARKPFNN"
     gene            13177..>13487
                     /locus_tag="PK518_09280"
     CDS             13177..>13487
                     /locus_tag="PK518_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM49147.1"
                     /translation="MAFLNGVHHVAYRCKNAKQTVEWYQKYLDANFILAIAENEVPST
                     KEPDPYMHIFIDIGGGNILAFFELPTKAEMIAPSDANTPSWTQHLALKVDSVETMMKV
                     KA"
BASE COUNT         2342 a         4578 c         4309 g         2258 t
ORIGIN      
        1 acgcgccgcg ctggccggcc cgatctgggc tggcacaccg ccggcagccc gcgccgacgc
       61 gctggcccgc gccgccgacc tgctggagca gcgcagccag ccgctgatgg gcctgatcca
      121 gcgcgaggcc ggcaagaccc tgcccaacgc cgtggccgaa atccgcgagg cagtggactt
      181 tctgcgctac tacggcgccc aggtggcggc cgaattcgac aacgccagcc agcgcccgct
      241 gggcgtggtg ctggccatca gcccatggaa ctttccgctg gccatctttg ctggccaggt
      301 ggccgcggcc ctggccgcag gcaacaccgt gctggccaag ccggccgagc aaacgccgct
      361 caccgccgcc gccatggtgc aaatcctgca cgaggccggc gtgccgcagg acgcgctgca
      421 gctcgtgccc ggccgcggcg agagcgtggg cgcggccctg gtggcgcacc cgcaggtcgc
      481 cggcgtcatg ttcaccggct cgaccgaggt ggcgcgcatc atcgcgcgcc agctggccaa
      541 gcgcctgtcg cccagcggcc aggcgattcc gctgatcgcc gagaccggcg ggcaaaacgc
      601 catgatcgtg gattcctcgg ccctggccga gcaggtggtg ggcgacgtgc tggccagcgc
      661 gtttgactcg gccggccagc gctgctcggc gctgcgcgtg ctgtgcctgc aggaagacgt
      721 ggccgagcgc accctgcaca tgctgcgcca cgccctgcag gagtggaaca tgggcaaccc
      781 ggaccagctg tcgaccgacg ttggcccggt gatcgacgcc gaggcgcgcg cccagatcga
      841 ggcgcatatc gagcgcatgc aggcggccgg ccagaaggtc acgcgcgtgc agcgcggcaa
      901 cggcgccgcg ctcaacggcc acttcgtggc cccggccatc atcgagatcg acagcccggc
      961 gcgcctgacg cgcgaggtct tcggcccggt gctgcacgtg attcgcttcc aacgtgagca
     1021 gctcgaccag ctgatcgacg gcatcaacgc caccggctac ggcctgactt ttggcatgca
     1081 cagccgcatc gacgagacca tcgcccacct gaccgagcgc gtgcacgccg gcaacatcta
     1141 tgtgaaccgc aacgtcatcg gcgccgtggt cggcgtgcag ccctttggcg gcatgggcct
     1201 gtcgggcacc ggccccaagg ccggcggccc gttgtacctg caccgcctgg tgcaaggcaa
     1261 ccccaacgcc gcgctggcca gcgtgcccac ggccgaggcc gcaccggcca gccgggcgct
     1321 gctcgaacgc ctgacgcagg ccaacctgcc aggcgccagc gccacgcagc tgcgcgcgct
     1381 gatcgacgcc gccctggccg ccacgcgcgt gggcgccagc tggctgctgc ccggccccac
     1441 gggtgaatcc aaccgctacc gcctgctgcc gcgcggcgcc gtctgggcgc tgccggccag
     1501 caccctgggc ctggtgcacc aggtggtggc ggcactggcc agcggcaacc cctgccatgt
     1561 ggtgctaccc gagcaggaca gcggctgcac cgcgctgtgg caggcgctgc gccaaagcgc
     1621 tggcgccgag ggcacggcct ggctgcacag cgccgaggca gccagcctgg acgacgcggc
     1681 cacgcccatg gcggcgctgc tgttcgaggg cgacggcgac gcgctgctgc aggtggcggc
     1741 gcgcgtggcc gcgcgtgacg gcgccatcgt gcgtgttgaa agccgctcca gcgacgagct
     1801 ggccaccggc cgcagctacg acctggccgc gctggtgcac gagcagtcga tcagcaccaa
     1861 caccgcggcg gcgggcggca acgcccagct catgaccatg gcctgatgcg cgttgcgccg
     1921 gtttgacccc ctctccctct gggagagggt gggggtgagg gccggcagcc gtatctgaaa
     1981 catgccgtca agcaattaaa taggctgcat ctcttttaaa taaagcgttt gtagctatca
     2041 aaacgagagt aaatcatgca atacaactgg aacgacccga cggcgatcat gttcgccgtc
     2101 tacctggtcg ccatgctggg cattggcgcc ctgggctacc tgggcaccaa gaacctgtcg
     2161 gactacatcc tgggcgggcg cagcctgggc agctttgtca cggcgctgtc ggccggcgcg
     2221 tccgacatga gcggctggct gctcatgggc ctgcccggtg cggtctatct gtcgggcctg
     2281 tcggagtcgt ggattgccat cggcctggtg gcaggcgcct acctcaactg gcgcctggtg
     2341 gcggcgcgcc tgcgcctgta caccgagcgc gccggcaacg cgctgaccct gcccgactac
     2401 ctggccaacc gctttgagga caagggcaac ctgctgcgca tcgtcaccgc cctggtgatt
     2461 ctggtgttct tcacgctgta ctgcgcctcg ggcgtggtcg ccggtgcgcg cctgttcgaa
     2521 aacatgttcg gcatgcccta cgcaaccgcc ctgtgggtcg gcgcggcctg caccattgcc
     2581 tatgtgctga tcggcggctt tctggccgtc agctggaccg acaccatcca ggcctcgatg
     2641 atgattaccg cgctgatcct ggcgccggtc atggcctatc tggccgtcga aggccagctg
     2701 cagcccggcc aggactggag cgccatcgtc agcgccgaga agttcgacct gttcaagggc
     2761 gcaagcttcg tcggtatcgt ctcgctgctg gcctggggcc tgggctactt cggccagccg
     2821 cacatcctgg tgcgcttcat ggccgcgtcg tcggtgcaga ccattcctgc cgcgcgccgc
     2881 atcagcatga cctggatgat tctgtgcctg atgggcgccg tggccgtggg ctttgtcggc
     2941 atccccttct ttgccgcgca cacgagtggc gcggcggccg tgaacgccaa cgccgagacg
     3001 gtgttcatgg aagtctccaa gcagctgttc aacccctgga tcgccggcgc cctgctggcc
     3061 gccattttgg ctgcggtgat gagcacgctg tcgtgccagc tgctggtgtg ctcgagcgcc
     3121 ttgaccgagg acatctaccg cgtcttcctg cgcaagaacg ccagccagac cgagctggtc
     3181 tgggtcggcc gcatcatggt gctggtggtc gcgctgattg cgattgccat cgccagcaac
     3241 cccgaggcca aggtgctggg catggtcagc tacgcctggg ccggctttgg cgcggcgttt
     3301 ggcccggtca tcatcctgtc gctgttctgg ggccgcatga cgcgcggcgg cgcgctcgcc
     3361 ggcatcatcg tcggcgccgt cacggtgctg gtgtggaagc agttcgcgct gtggggcctg
     3421 tacgaaatcg tgcccggctt catcctgggc tgggtgtcgg tggtggtggc aagcctgctg
     3481 agtgccgagc catcaaagag catgcgccag gcccatgcta cggtggagcg tgaatttgcc
     3541 cagatcagcc aaggccgatg ggtcagcgcg acatgaaata ggtaaacccg accgatacca
     3601 ggcccggtac tgcgcagcag gccgggcctt ttgctttgca gcctgcaggt ttgcgtttca
     3661 cctcgtaaaa tgcaaccgtt accaaccgtt ttcattgcca tgaagaaaac cccgacggat
     3721 gtccaaccca aacccagcgt gcaggtgctg gagcgcatgt tccagttgat cgatgtgctg
     3781 gcgtccaggg aggagtccgt ctcgctcaag gaaatcagcg aacgcaccgg cctgcacccg
     3841 tccacggcgc accgcatcct caacgacatg acgataggcc gcttcgtgga tcgccccgaa
     3901 tcgggcagct accgcctggg catgcgcctc ctggagctgg gcaacctggt caaggcgcgc
     3961 ctgtcggtgc gcgacgccgc catggccccg atgcgccact tgcacaagct gatacagcag
     4021 ccggtgaacc tgagcgtgcg ccagggcgac gagatcgtct atgtcgagcg cgcctacagc
     4081 gagcgctcgg gcatgcaggt ggtgcgcgcc attggcgggc atgcaccgct gcacctgacc
     4141 tccaccggca agctgtttct ggcctcggaa gacacgcagc gcgtgcgcgc ctacgccacg
     4201 cgcaccggcc tgcccggcca tacgcgcaac agcatcaccc aattgccaat gctggagcgc
     4261 gaactgagca aggcgcgcca gtacggcatt gcgcgcgaca acgaggagct ggagctgggc
     4321 gtgcgctgca tggccgccgg tgtgttcgac gaccaggggc agctggtggc cggcttgtcg
     4381 atctcggcac cagccgatcg cctggacgac ggctgggtgc ccaagctgca ggccgcggca
     4441 caggagatct cgctggcgct gggctacagc acgggtcagc gcaccgtgct ccagggggtg
     4501 tgaggactga gcctgagcgc caccgttttt ccaggtggcg ccgcagctcg gtcagctacg
     4561 cggcgtggca gccgcgccca ggctgatgct gctgccgggg tgcatcttct cgacccagga
     4621 gcgcacccgc tcggcatcgc ccaggcggct gaacttgccc gccgaatcca ggaacaccat
     4681 gatcagcttg cgccccgcca cctcggcctg catcaccagg cagcgaccgg cctcggagat
     4741 gtagccggtc ttctgcagac cgatgtccca ggtcgggctc ttcaccaggc ggttggtatt
     4801 gttgtactgc agcacgcggc tacccacggt gacctcgtgc cccggggagg tggacagctc
     4861 gcgcagcagt gagtcgccat gtgccacgtt gaccagcgtg gccaggtcat gggcgctgga
     4921 ctggttgctg ctggacaggc cggtgggctc gacaaagcgc gattccgtca tgcccagcag
     4981 ccgggccttg gcattcatgc gctgcacgaa ggccgtcagg ccgccgggat aggtgcgccc
     5041 cagcgcgtgc gcggcgcggt tctcgctgga catcaaggcc aggtgcaaca gttctccgcg
     5101 cgacagcgtg gtgcccacgg tcaggcgcga cccgctgccc ttttcggtgt ccacatcgtc
     5161 ctgcgtgatg gtgatgggct cgtccatggg caggcgcgcc tcggaaacga gcaggccggt
     5221 catcagcttg gtcagcgagg caatcggcag caccgcatgg tcgttcttgc tcagcaacac
     5281 ctccttggtg tcctgatcga tcaccagcgc cacgctggac ttcagatcca gcgggtcact
     5341 gaccgaatgc agtcccgcca gctgaccaaa ggacaggcgt gcgggctcgg ccgccatgcg
     5401 caccggcgcc ttggcgctac gcgctgccat gcgcgtggac ttgaccgaac cggccttgcg
     5461 ctgcgccttg gcgctcgatt tcacggccgc acgcttggcc accacctgcg actgtttctt
     5521 ggaggcagcg ggtttgcgtt cggcggcctg cgcaccggga aatgccaggg acaggcccag
     5581 ggctgcaatg gccagaactt gggaaaaacg ggttgcgatg cgaaaacggg cgtgcatcag
     5641 aggctccgaa cgtaagaaac tgtacaccag tgtacagaat caaaaaaagc cgcgcaagat
     5701 caatatcttg cgcgactctt ttaaaaagaa cctgaattat atgggtaacc aggctttaac
     5761 cctgggctgc cacacgctcg gctttgcttt gcagcttgtt cagggcactc aggtaggcct
     5821 tggccgaggc cacgacgatg tccggatccg cgcccacgcc attgacgatg cgaccggtgt
     5881 tctgcaggcg caccgtgacc tcgccctggc tttcggtgtt gccgctgatc gccgtcaccg
     5941 aatacagcac catttccgcg ccacttttca cctggctctc gatggccttg agcgaggcat
     6001 cgaccgggcc gttgccgtcc gatgtgccac gcacctcgcg gccatcgatg gtgaagacga
     6061 tctcggcatg cggcatctcg ccggtctcgc tgcgctgggc cagggagatg aaaccgtatt
     6121 gctccttctc cgccgtcacg ctttcgtcgc cgaccagggc caggatgtcc tcatcaaaga
     6181 tctcgctctt gcggtcggcc agatccttga acttggcgaa ggccgccatg atctcgccct
     6241 cggattcgag caccacgccc agctcctgca gccgctgctt gaacgccgtg cgcccggaca
     6301 gcttgcccag cacgatcttg ttggcgctcc agcccacgtc ctcggcgcgc atgatctcgt
     6361 aggtgtcgcg cgccttgagc acgccgtcct ggtggatgcc gctggcgtgg gcaaaggcat
     6421 tcgcccccac caccgccttg ttgggctgca ccacgaagcc ggtggtggtg ctgaccatcc
     6481 ggctggccgg cacgatctgc gaggtgtcta tgcccacgtc caggccaaag tagtcgcggc
     6541 gcgtcttcac ggccatgacg atttcctcga gcgagcaatt gcccgcgcgc tcacccaggc
     6601 cgttgatggt gcattcgacc tggcgcgcgc cgccgatctt cacgcccgcc aggctgttgg
     6661 ccacggccat gcccaggtcg ttgtggcaat gcacgctcca gatggccttg tcgctgttgg
     6721 gcaccttctc gcgcagcgtc ttgatgaagt tgccgtacag ctcgggaatg gcatagccca
     6781 cggtgtcggg gatgttgatg gtggtcgcgc cctcgttgat caccgcctcg cacacgcgcg
     6841 ccaggaagtc gatctcgctg cggtagccgt cctcggcgct gaactcgatg tcgcccatca
     6901 ggttgcgcgc aaagcgcacc gccagcttcg actgctccag cacctcctcg ggcgtcatgc
     6961 gcagcttttt ctccatgtgc agcgggctgg tggcgatgaa ggtgtggatg cggccgcgct
     7021 cggcgtccct gagcgcctcg gcggcgcgcg cgatgtcgcg gtcgttggcg cgcgagagcg
     7081 agcagatggt cgagtccttg atggcgcgcg caatcgcctg cacgcactcg aagtcgccat
     7141 tgctgctggc ggcaaagccg gcctcgatca cgtccacctt caggcgttcg agctggcgcg
     7201 cgatgcgcag cttctcgtcg cgcgtcatgc tggcgccggg cgactgctcg ccgtcgcgca
     7261 aggtggtgtc gaagatgatg agtttgtcgc tgctcatggc tgcctctgtc gtggtgaata
     7321 ttcgatatta agcggctacc acctgcatct gcggtgcacg ctgcagaccg ctgaggatgg
     7381 cgcgcatgga ggcggagacg atgttggcgt ccatgcccac gccgtacacc gtttggcgct
     7441 cgtcgatgcg catctccaca taggccacgg cctgggcgtt ggcgccctcg cctatggcgt
     7501 gctctgtgta atcgagcacc cgcagctggc gatcgagcgc cgtcgaaagg gcctgcacga
     7561 aggcatcgat gggtcctgtg ccctcgcctt ccaagtccaa gatgtcgccc tgccactgca
     7621 ccgtggccgc cagctgcatg cggccgccat cgtcctgcat gcgataggcg ggcgcggcca
     7681 cggcttggat ctgatattcg ctctggaaca gcgcccacag atcggccgcg gtcagctcct
     7741 tgccgctggc gtccatttcg cgctgcacgg cggcgctgaa ttcgatctgc aagcggcgcg
     7801 gcaactgcag gccgtattcg ctctccagca ggtaggcgat gccgcccttg cccgactggc
     7861 tgttgacgcg gatcacggcc tcgtagctgc ggcccaggtc cttggggtcg atgggcagat
     7921 agggcatgtc ccagatgtcg ccttccttgc gctcggcaaa ggccttcttg atcgcgtcct
     7981 ggtgcgagcc cgagaacgag gtgtagacca ggtcgccaac ataggggtgg cgcggatgca
     8041 cgggcaactg gttgcagtat tccaccgtgc tgcggatggc gtcgatgtcg gaaaaatcca
     8101 gccccgggtg cacgccctgg gtgtacatgt tgagggccac gtttactacg tcaaggttac
     8161 cggtgcgctc gccgttgccg aacaagcagc cctccagccg gtcggcgccc gccatcagcg
     8221 ccagttcggc cgtggccgtg ccggtgccgc ggtcattgtg cgggtgcacc gacagcacca
     8281 cgctgtcacg gcgcgcgatg tggcggtgcg tccactccac catgtcggca aagatgttgg
     8341 gcgtggaatg ctcgaccgtg gtgggcaggt tgatgatgca tttcttctcg ggcgtgggct
     8401 gccagaccgc ggtcaccgca tcgatcacgc gcttggaaaa tgccagctcg gtgtccgaat
     8461 acatctcggg cgaatactcg aaggtccagt cggtcgccgg ctggcgtgcg gccaagtcct
     8521 ggaacatctg cgcgtggctc acggccagct ccacgatctc gtcctcgctc atgcccagca
     8581 ccacgcggcg catcaccggc gcggtggcgt tgtacaggtg cacgatggcg cgcggcgccc
     8641 cggcaagcgc ctcaaacgtg cggcggatca gcggttcgcg cgcctgggtc agcacctgga
     8701 tggtgacgtc gtcgggaatg cggtcttcct cgatgagctt gcgcacgaag tcaaactcga
     8761 cctgcgaggc ggagggaaat ccgacctcga tctccttgaa gccgatcttc accagctgct
     8821 cgaacatgcg catcttgcgc gcaatgtcca tgggctcgat cagcgcctga ttgccgtcgc
     8881 gcaaatccac gctgcaccag atgggggcct gggtgatcac cgcgtcgggc cagcggcgat
     8941 cggccaggcg cacgggcgcg aaggggcggt atttgctggc ggggttgtgc aacatggacg
     9001 gactcgcttt catggaacag gaaaagatga aacccggttc cagcgacttg cacaaacaaa
     9061 aacggcccgt cgctggtgct cacgggccgt ggaagtagtg tgtgtacgcg cgcgctactc
     9121 tatgcctgcc cgtgggacaa gttctgtagt agtgctagcg acaggaaatg catagcgaca
     9181 gactctagca caaacgcgag gactgcaatt gatcctccag aaatgtgcgg aattcctgcg
     9241 ccgccggcga cagcgccctg ccccggcgcg tgaagaccca gaagctgcgc tccaccaccg
     9301 ggtggcccac cgggcgcatg accaggccga actgctcgac cagcgtgcgc gcatagggca
     9361 tgcacagcgt gatacccagg cccgcacgcg ccagcgccag cgccgtggtc atataggtca
     9421 cctgcatgaa ggcctccttg tgcatgtcgc gcgctgcctc gcccacgtcg ctcaccagca
     9481 gctcggtgaa ctggccctgc agcgtgatca gcgactggcc ggacaggtct ggccacagca
     9541 gcttcttgcg ccgcgccagg gcatgggtgg gcggcatcac ggccatgaag ggcaggctga
     9601 acagctgctc agcggcgatg tctgaattgg cctcgcgctc cgggcctatg ccgatgtcca
     9661 cctcgccgga gaacacgcgc gccatcacgc tctcgaccgc gcaatcgacc aggcgcaggc
     9721 tcacgcccgg gtactgggcg cgaaagcccg ccatcagctc gggcagcagc gtgcaggcca
     9781 gcagctgcgg cacggcgacg cgcaccgtgc cgcgctgcaa gtccttgagg ttgccgacct
     9841 cgctgaccac gccgtccagg tcgtgcagga ttttctcgat ctgcgggtac agctcgcgcc
     9901 cggtgtctga caggcgcagg cggcgcgtgc tgcggtcgaa caggcgcagg cccagggtcg
     9961 attccagctc cttgatcagg ccgctgagcg ccgactgggt gatgaacaag cgctctgccg
    10021 ccagcgtgaa gctgccggta tcggccacgg ccacgaaggc gcgcaactgg cgcagcgtca
    10081 cattcatgag tcaatccgat aaatagatca ataaataccg tttgaataat aaatcaatcc
    10141 ggcctcaaat acaccccatc gcacccttac cacaaaactc aggagccaac gccatgaccg
    10201 acaaaaaatt tgcctcccag gccgatctgg aagaaaagaa gatcagctgg gaaaaactca
    10261 gcgacaacgc ctacgcctac acggccgagg gcgacccgaa caccggcgtc atcatcggcg
    10321 atgacggctg catgatcatc gacgccaccg ccaccccggt ggccgcgcag ggcgtgatcg
    10381 ccaagatccg cgagatcacc gacaagccca tcaagtacgt ggtgctgacg cattaccacg
    10441 ccgtgcgcgt gctcggcgcc tcgggttaca aaacggaagg cctggagcag atcatcgcca
    10501 gccgcgacac ctacgacctg atcgtcgagc gcggccagca ggacatggat tccgagatcg
    10561 gccgcttccc gcgcctgttc caggccgtgg agagcgtgcc cggcctgacc tggccgacca
    10621 tgaccttcga gggcgagatg gccctgtggc tgggcaagct ggaggtgcgc atcaagcagg
    10681 tgggccgcgg ccacaccaag ggcgacgcca tcgtctacat ccccagccag aacatctgct
    10741 tctcgggcga cctggtcgag gccgacgccg cctgctacac cggcgacgcc tacctggccg
    10801 actggccgca gacgctggac aacctggcgg cgatgaagtt cgacaagctc atccccggcc
    10861 gtggcccggc gctgatgacg ccagcggacg tgcaaaaggg cctggcctat acccgcgact
    10921 ttgtctccac gctctacaag agtgcgcaag aggccgtcgc ccaagggatg gacttgaacg
    10981 ccaccatgaa gcacacgcgc caggccatgg acccgaagtt tggccatgtc ttcatctacg
    11041 agcactgcct gccgtttgac gtgacgcgag cgcacgacga ggccagcggc atccgcgacc
    11101 cgcgcatctg gacggccgag cgcgaccagc agatgtggca tgcgctgcaa caagcctaaa
    11161 taaaaacaat agctgtcaac gcttaccaga taagcgctag agcgatattt caccatcttt
    11221 gaggattcga gagacacatg ctgaagacct acacctatcc caagttcgac tatgtgcgcc
    11281 cacccgagct ggtggagcag cacagcggcc actaccccgt ggtcatcgtc ggcgccggcc
    11341 cggtgggcct gtccatggcc attgacctgg cggcccaggg gcaaaaagtg ctgctgctgg
    11401 acaacgacga caccgtgtcc ataggctcgc gcggcgtgtg ctatgccaag cgcacgctgg
    11461 aggttctaga ccgcatggac tgcggcagcc cctgcgtgga caagggcatg tcctggaacg
    11521 tgggccgcac cttctttggc gagggcgagg tcttcaactt caacctgcgc ccgcaggacg
    11581 gccacaaccg ccccggcatg gtgaacctgc agcagtacta cctggaggac tacctggtac
    11641 agcgcgcgcg ccagttgccc aacctggaga tgcgcttcaa gaacaacgtc atcagcgtgg
    11701 agcaggacga caaacaggcc accgtgcgcg tggagacgcc cgacggcgcc tacaccctga
    11761 ccactgattg gctggtggcc gccgacggcg cgcgctcgcc gatccgcaac atgctgggcc
    11821 tggagatcga cggcaagatc ttcatggacc gcttcctgat tgccgacgtc atcatgaagg
    11881 ccgactatcc cgcagagcgc tggttctggt tcgacccgcc cttccatccc aaccagtcgg
    11941 tgctcttgca caagcaggcc gacaacgtct ggcgcatcga cttccagctc ggctgggacg
    12001 ccgaccccga ggccgagaag aagcccgaga aggtcatccc gcgcatcaag gccatgcttg
    12061 gcgacgagcg cgaattcgag ctcgaatggg tcagcgtcta caccttccag tgccggcgca
    12121 tgggccagtt caaccacaac cgcgtgctgt tcgtgggcga tgcggcgcac caggtctcgc
    12181 cctttggcgc acgcggcgcc aacagcggca tccaggactc ggacaacctg gcctggaagc
    12241 tcaagctcgt catcgacggc aaggcgcccg cgtcgctgct ggacagctac agcagcgagc
    12301 gctgctacgc cgccgacgag aacatcatga actccacgcg ctccaccgac ttcatcacgc
    12361 ccaagagcaa gaccagcaag gcttttcgca acgccgtgct ggagctggcg caggactacc
    12421 cgtttgcccg cgccctggtc aactccggcc gcctgtcggt gcccagctgg ctcgtcgatt
    12481 cgccgctgaa cacccccgac gaagacggct ttgccggcaa catggtgccc ggcgcgccca
    12541 tggacgacgc ccccgtcacc ggcggcaaga ccgcctggct gctgggcgac atggacacgc
    12601 gctttcacct gctgcactac gtggaagacg ccggcgcgct gagcgcgcag caggcccagg
    12661 ccctggccgg cctggccgac caccccatag ccgtgcacgc catcgtcgtg gcccagcgcg
    12721 gcaccgcccc cgccggcctg gccacgctga tcgacgccca gggcgcgctg cgcgagcgct
    12781 acgacctgca gcccggcacc agctacctgc tgcgcccgga ccagcatgtg gctgcgcgct
    12841 ggcgccagct tgatgctgcc aaggtgcgcg ccgccgtgaa ccgcgccacc ggcaacgtct
    12901 aacaccaaag gagacaacca tgagcctgaa cacccagccc aacctgcgcg agcccggcaa
    12961 gcgctacttc caggcctatt cgcccggcga cgacttctac gaggccctga tcgacaccca
    13021 ccagggcctg tcggacgagc aaagcgccgc cctgaacgcg cgcctgatcc tgctgctgtc
    13081 caaccatatt ggcgacatca ccgtgctgcg cgaggccctg gccatcgccc gcaaaccctt
    13141 caacaattga caccgccagc ccccaaggag acaaccatgg cctttctcaa cggcgtacac
    13201 catgtcgcat accgctgcaa gaacgcaaag cagaccgtgg agtggtacca gaagtacctc
    13261 gatgccaact tcatcctcgc catcgccgag aacgaagtgc cctccaccaa ggagcccgat
    13321 ccgtacatgc acatcttcat cgacatcggg ggcggcaaca tcctggcctt cttcgagctg
    13381 cccaccaagg ccgagatgat cgccccctcg gacgccaaca cgccgtcgtg gacgcagcac
    13441 ctggcgctga aggtggattc ggtcgaaacc atgatgaagg tcaaggc
//
LOCUS       DAOMTQ010000083        13450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_784_length_13450_cov_27.602912, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000083 DAOMTQ010000000
VERSION     DAOMTQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_784_length_13450_cov_27.602912"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PK518_09285"
     CDS             <1..908
                     /locus_tag="PK518_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM49148.1"
                     /translation="ACITHGSTIVYPNDGFDPLSVLETVQAERCTGLHGVPTMFIAEL
                     DHPRFKEFDLSSLRTGIMAGSPCPIEVMKRVQADMHMTEVTIAYGMTETSPVSCQSDA
                     DTPLEKRVSTVGRVQPHLEVKIIDPETGATVARGERGELCTRGYSVMHGYWGDVEKTR
                     EAIDPEAWMHTGDLATMDDEGYVNIVGRIKDMVIRGGENLYPREIEEFLYRHPQVQDV
                     QVVGVPDEKYGEELCAWIIAKPGSSPTEDDIRAFCKGQIAHYKVPRYIRFVQAFPMTV
                     TGKIQKFKIRDVMKDELGLQESKTA"
     gene            931..2535
                     /locus_tag="PK518_09290"
     CDS             931..2535
                     /locus_tag="PK518_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRM49149.1"
                     /translation="MILESQINPRSAEFQANAAAMRAVVDDLRAHVERVAQGGGETAR
                     AKHVARGKLLPRERVEMLLDPGTPFLEIAPLAALNMYGNDAPGAGLIAGIGRVSGVDC
                     MIVCNDATVKGGTYYPVTVKKHLRAQEIAQQNCLPCIYLVDSGGANLPNQDEVFPDRD
                     HFGRIFYNQANMSAQGLAQIAVVMGSCTAGGAYVPAMSDETIIVKNQGTIFLGGPPLV
                     KAATGEVVTSEDLGGGDVHTRLSGVADHLAQSDLHALALARSAVANLNRKQPETQADT
                     APTAPKYEASELYGVIPVDTRKPFDVREIIARIVDGSAFDEFKARYGNTLVCGFARIE
                     GMAVGIIANNGILFSESAQKGAHFIELCCQRKIPLVFLQNITGFMVGRKYENEGIARH
                     GAKMVTAVATANVPKFTIIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQAA
                     SVLATVKRDGIEGKGGKWSAEEEEAFKAPIRDQYEKQGHPYYATARLWDDGIIDPADT
                     RRVLALALATSRHAPIEDVKFGVFRM"
     gene            2545..3324
                     /locus_tag="PK518_09295"
     CDS             2545..3324
                     /locus_tag="PK518_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRM49150.1"
                     /translation="MTALTIRYDGPRATITLTQPEIRNAFSDEVIAEITAAFTEVGAR
                     ADVRAVVLAAEGPAFCAGANLNWMRRMADYTREQNLADAAKLAEMLRVIYECPKPTIA
                     RVQGDVYAGGTGLVAACDMAVSVDTAGFCLSEVKIGLIPATISPYVIRAMGARAAHRY
                     FLTAERFGAQEALRIGFVHEVVTAEQLDAKVDELVKALISASPNAVRACKKLVLDVAE
                     REINAALIAATVEGIADIRASDEGREGVQSFLQKRKPSWLA"
     gene            3333..4052
                     /locus_tag="PK518_09300"
     CDS             3333..4052
                     /locus_tag="PK518_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRM49151.1"
                     /translation="MDALWLQIVQWLHTVGLHIDSGTAQAVAEGAARATQQLDMPGLL
                     ALAAALGWASGMRLYAVVFLVGGMGALGWIALPPALTVLQHPVVLMASGFMLLVEFFA
                     DKVPWLDSAWDAVHAFIRVPAGALLAAGVFGADNASMAVAAGLLGGTLSATALATKMT
                     ARAAANTSPEPFSNWGLSFLEDGLVVATVWLATQHPLAFGVALVLALAVSALLLVILF
                     KFLRAVLRRVSSLFSASAKVA"
     gene            4053..6074
                     /locus_tag="PK518_09305"
     CDS             4053..6074
                     /locus_tag="PK518_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HRM49152.1"
                     /translation="MFTKILIANRGEIACRVAATAKRMGVKTVAVYSDADAQAKHVAA
                     CDEAVHIGASAPKDSYLRWERIIEAAQQTGAQAIHPGYGFLSENEDFAQACAKAGLVF
                     IGPPASAISAMGLKAESKRLMAKASVPLVPGYQGEDQDPQLLQREADAIGYPVLIKAS
                     AGGGGKGMRLVEKSEDFAAALESCKREAINSFGNDAVLVEKYVLRPRHIEIQVFGDTH
                     GNCVYLFERDCSVQRRHQKVLEEAPAPGMTPELRARMGQAAVAAAQAVGYVGAGTVEF
                     IVEQPGGYDAPEAMKFYFMEMNTRLQVEHPVTEAITGEDLVEWQLRVASGQPLPKRQD
                     ELRIIGHAIEARICAENPDNNFLPATGRLNVYRKPVCTSFERSQVRVDDGVREGDAIS
                     PFYDSMIAKLIVHGDTREQALARLDDALAQTHIVGLATNVQFLRHVVQSGAFAQARLD
                     TALIQREEAVLFQQEKVGLPLAAAAAVASQLLRERAGQGANPFSRTDGWRSLTACKRR
                     FEFDFGGQHAKAWLSYERDGSYRLAVGEGEGAVSGALAFALAPGGAMDLQYAGQRTQA
                     LVYAQGESDHVFTPRGATRIEALDLLAHAGETAGEGGRLTAPMPGKVVSFAVQAGDKV
                     SKGQALAVMEAMKMEHTIAAPQDGVVAELLYAPGDQVTEGAELLRLSAV"
     gene            6112..7032
                     /locus_tag="PK518_09310"
     CDS             6112..7032
                     /locus_tag="PK518_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HRM49153.1"
                     /translation="MKITFCCTDTKAEPWLQGLAAALPQADISLWQPGAPQADYAVVW
                     APPQQFIDEQAGLKALFNIGAGVDALLKLRLPPGLRVVRLDDAGMAVQMAEYVCHALI
                     RHFREFDDYEASARAGRWSYRKPRERRDFPVGVMGLGVLGERVARAVAQFDFPVRGWS
                     RSPKQVAGVQCFHGAQGFNDFLAASRVLVNLLPLTPETQDIMNRDTLARLMPGAYVIN
                     VARGAHLVDDDLLALLDGGHVAGATLDVFRTEPLPAGHPFWSHPRITLTPHTSARTLR
                     DESIAQIAGKIAALARGEAIAGVVSLERGY"
     gene            7066..7974
                     /locus_tag="PK518_09315"
     CDS             7066..7974
                     /locus_tag="PK518_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRM49154.1"
                     /translation="MPHPSRVRIIDVGPRDGLQNEKTPVPADIKIGLVQRLQDAGLQE
                     IEVTSYVSPKWVPQMADNHAVMSGMARKAGVRYSVLTPNLKGWEAAVLDKPDEIVVFG
                     AASEAFSQKNINCSIAESIERFAPVVDAALAAGVAVRGAMSCTVGCPYEGEIAPERVA
                     YLAGLMRGIGVQRVDVADTIGVGTPLKVQRAIEATAQHFGIDDISGHFHDTYGQALSN
                     TLAALELGVWNYQSSIAGLGGCPYAKGATGNVATEDLVYMLQGMGIETGIDLDALVDA
                     GQYISDFLGRKPQSRVAVALLNKRAG"
     gene            8055..8285
                     /locus_tag="PK518_09320"
     CDS             8055..8285
                     /locus_tag="PK518_09320"
                     /inference="COORDINATES: protein motif:HMM:NF027267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HRM49155.1"
                     /translation="MTAVAKITSKGQTTIPADIRAALQVGPGDKLAWEVLEAGVVQVR
                     RVQPLDMEFLQAMEGTLDEWAGAADEEAYRDL"
     gene            8275..8619
                     /locus_tag="PK518_09325"
     CDS             8275..8619
                     /locus_tag="PK518_09325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM49156.1"
                     /translation="MIYERWQVVRVPFPFTDRLASKNRPALVLSAAAAFNAPAGHLVL
                     AMITSAGNAPWPLDCAIQDLAGAGLPAPSVVRCKLFTLDARLVRGSLGRLAPHDVQEI
                     EHSLGRLFGAAV"
     gene            8645..9148
                     /locus_tag="PK518_09330"
     CDS             8645..9148
                     /locus_tag="PK518_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRM49157.1"
                     /translation="MNPAAVSPLPEAVQRVAAALQAARHPHMPIMLDDAARTAQQAAD
                     ALGVQLGQIAKSIIFRRKSDDAAVLVITSGDRRVDEKKVDALVGKTGRADAEFVKAKT
                     GFSIGGVSPLAHAQAPVTLIDRELLRFEVIWAAAGHPHGVFQLRPQDLQAFTGAPLAD
                     VVQVVVS"
     gene            9145..9405
                     /locus_tag="PK518_09335"
     CDS             9145..9405
                     /locus_tag="PK518_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HRM49158.1"
                     /translation="MKLSTLIAARAQQISVTGQFHSNYTGVIPSPCLSICRMTRDGSH
                     CEGCFRTLDEIRTWSQAGAARRRAIWADLLRRAGVALPEELA"
     gene            9402..10043
                     /locus_tag="PK518_09340"
     CDS             9402..10043
                     /locus_tag="PK518_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRM49159.1"
                     /translation="MKEITCYLDFVSPYAWLAFERLPQALEGLSYRLSYRPVLLGALL
                     NAHGNPGPAGIVPKRDWTYRHVSWLGHSLGVGLDMPARHPFPPLPLLRLALECSDDGL
                     VNRFVAGSILRHVWQGGADALDPARLDALRALLAEQLRPDEAAPERAKQLLRANTELA
                     AQRGVFGVPMFEVDGHLFWGLDSLPMLRAYLDGDGWFAQHWQRCASVPQGLPG"
     gene            10053..11870
                     /locus_tag="PK518_09345"
     CDS             10053..11870
                     /locus_tag="PK518_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HRM49160.1"
                     /translation="MPNAFNFSASPFDCLTAEEQSLVRDNVDIAYYPEGAVVLDVGNA
                     PEHLFVIIKGYVTQTEGEEVLATYGPEDCFDGRGLVAGRVSSRFTVAEELVAYQLARS
                     TVKDLIARNRGFGALLFSDLGNKLSALAQRAEQHQMQSLTLARVDQAFLRPAHVVAAG
                     TDIVSVVRIFQDQRTTSVLVSGLPDGLGIFSNTTLQRAILDGRPLERITVGELASSPV
                     VTVRAAEQIGDAMALLLRARVHRLAVLGEDGQVLGILEALDLFSYLANHSHLITVQIE
                     EARDLPALEAAAAQITRLVAALHRGGTRIALVAQLVQQLNARLFERAWQMIAPPDLLA
                     NSCLFVMGSEGRGEQLLKTDQDNGLLLSDGYEPPADLQAICDRFSQALAGFGYPECAG
                     RIMLNNPEWRGTVTGFGDKVRGWLLQPQGESLMYLAIFMDAHAVAGKAQLLAEVRQRV
                     MQLATDNDALVARFAMAIDAFGGAAGWWNRLLSLGGDADLVNLKKAGIFPIVHGVRSL
                     ALARRIKATGTVERIAALQGEGQLDEAMAQELVQGLHFLMGLRLQAGLAELELQRPVT
                     GNVDPQRLSSLERDLLKDTLSAVKRFKALLHQRLRLDAV"
     gene            11867..12532
                     /locus_tag="PK518_09350"
     CDS             11867..12532
                     /locus_tag="PK518_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM49161.1"
                     /translation="MSRHYSQAAPLWWEKLRRWWLIYHLGEPEFAPMFDPPPHDEWVA
                     LDCETTGLNTRSDEIISVGAVRIVGDRIMTSERLELLIRPEKGVSADSVRIHRLRERD
                     LEGGLPKDEAMRRLMRFIGSRPLVGYYLEFDVAMLNRAIWPLLGMGLPQPKIEVSAMY
                     YDYKFKQLPPYQQQATTEIDLRFATLMQDLALPEREAHDALNDAVMAAMAFIKLRRLR
                     GEA"
     gene            complement(12662..>13450)
                     /gene="actP"
                     /locus_tag="PK518_09355"
     CDS             complement(12662..>13450)
                     /gene="actP"
                     /locus_tag="PK518_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059048.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRM49162.1"
                     /translation="RKSVGWATVWIGYFYILIFIIGFGAITMVLTNPEMADTVKGVIK
                     GGAGTANMAAVLVAKAVGGNIFYGFISAVAFATILAVVAGLTLSGASAVSHDLYSTVI
                     KKGKADSAAELKVSRMTTLALGVVAVILGIAFEKQNIAFMVSLAFAIAASANFPALIL
                     SILWKGCTTRGAVFGGFLGLISSVGLTVVSPSVWEATLGNPAGSALFPYSSPALFSMT
                     IGFVGVWLFSITDSSARAAKDRAAFPAQQVRSETGLGASGASGH"
BASE COUNT         2249 a         4509 c         4532 g         2160 t
ORIGIN      
        1 tggcttgcat cacgcatggc agcaccatcg tctatccgaa cgacggcttt gaccccttga
       61 gcgtgctcga gaccgtgcag gccgagcgct gcaccggcct gcatggcgtg cccaccatgt
      121 tcatcgccga gctggaccac ccgcgcttca aggagttcga cctgagcagc ctgcgcaccg
      181 gcatcatggc cggcagcccc tgcccgatcg aggtcatgaa gcgcgtgcag gcggacatgc
      241 acatgaccga ggtgaccatt gcctacggca tgacggagac cagccccgtc agctgccaga
      301 gcgacgccga cacgcccctg gaaaagcgcg tctccaccgt cggccgggtg cagccgcacc
      361 tggaggtgaa gatcatcgat cccgaaaccg gcgccactgt ggcgcgcggc gagcgcggcg
      421 agctgtgcac gcgcggctac tcggtcatgc atggctattg gggcgacgtg gagaaaacgc
      481 gcgaggccat agaccccgag gcctggatgc acaccggcga cctggccacc atggacgatg
      541 agggctatgt gaacatcgtc ggccgcatca aggacatggt gatacgcggc ggcgagaacc
      601 tctacccccg cgagatcgaa gaatttttgt accgccaccc ccaggtgcag gatgtgcagg
      661 tggtcggcgt gccggacgag aaatacggcg aggagctgtg cgcctggatc atcgccaagc
      721 ccggcagcag cccgaccgag gacgacatcc gcgccttctg caagggccag atcgcgcact
      781 acaaggtgcc gcgctacatc cgttttgtgc aagccttccc gatgaccgtg accggcaaga
      841 tccagaagtt caagatccgc gacgtgatga aggatgagct gggcctgcag gaaagcaaga
      901 cggcataaat ccaaaccttt cggagcaacc atgattctcg aatcccagat caatccgcgc
      961 tcggcggaat tccaggccaa cgccgccgcc atgcgcgccg tggtcgatga cctgcgcgcc
     1021 catgtcgagc gcgtggccca gggcggcggc gagaccgcgc gcgccaagca tgtggcgcgc
     1081 ggcaagctgc tgccgcgcga gcgcgtggag atgctgctcg accccggcac gccctttctg
     1141 gagatcgcgc cgctggccgc gctcaacatg tatggcaacg atgcgcccgg cgccggcctg
     1201 atcgccggca taggccgcgt ctcgggcgtg gactgcatga tcgtctgcaa cgacgccacg
     1261 gtgaagggcg gcacctacta tcccgtcacc gtcaagaagc atttgcgcgc gcaggagatc
     1321 gcgcagcaga actgcctgcc ctgcatctac ctggtggact cgggcggcgc caatctgccc
     1381 aaccaggatg aggtcttccc ggaccgcgac cacttcggcc gcatcttcta caaccaggcc
     1441 aacatgagcg cccagggcct ggcgcagatc gccgtggtca tgggcagctg cacggccggc
     1501 ggcgcctatg tgccggcgat gagcgacgag accatcatcg tcaagaacca gggcaccatc
     1561 ttcctgggcg gcccgccgct ggtgaaggcc gccacgggcg aggtggtgac gagcgaagat
     1621 ttaggcggcg gcgacgtgca cacgcgcctc tcgggcgtgg ccgaccatct ggcgcaaagc
     1681 gacctgcatg cgctggcgct ggcccgttcc gccgtggcta atttgaatag aaaacagcct
     1741 gaaacgcagg cggatactgc gccaacagct cctaaatatg aagcaagcga gctgtatggc
     1801 gtgatccctg tcgatacgcg caagcccttt gacgtgcgcg agatcatcgc ccgcatcgtc
     1861 gatggcagcg cgttcgacga gttcaaggcg cgctacggca acaccctggt ctgcggcttt
     1921 gcgcgcatcg aggggatggc cgtgggcatc atcgccaaca acggcatcct gttcagcgag
     1981 tcggcgcaga agggcgcgca cttcatcgag ctgtgctgcc agcgcaagat accgctggtg
     2041 tttttgcaga acatcaccgg cttcatggtc gggcgcaagt acgagaacga gggcatcgcg
     2101 cgccacggcg cgaagatggt cacggccgtg gccacggcga atgttccgaa gttcaccatc
     2161 atcatcggcg gcagctttgg cgccggcaac tacggcatgt gcggacgcgc ctacagtccg
     2221 cgctttttgt ggatgtggcc gaacgcgcgc atctcggtca tgggcggcga gcaggcggcc
     2281 agcgtgctcg ccaccgtcaa gcgcgacggc atagagggca agggcggcaa gtggtctgcc
     2341 gaggaagagg aggccttcaa ggccccgata cgcgaccagt acgagaagca gggccacccc
     2401 tactacgcca cggcgcgcct ttgggacgac ggcatcatcg acccggccga cacgcgccgc
     2461 gtgctggccc tggccctggc cacctcgcgc cacgcgccga ttgaagacgt gaagttcggc
     2521 gtgttccgca tgtaaggaga caccatgaca gccttgacga ttcgctacga cggcccgcgt
     2581 gccaccatca cgctgaccca gcccgagatc cgcaacgcct tcagcgacga ggtcatcgcc
     2641 gagatcaccg ccgccttcac cgaggtgggc gcacgcgccg atgtgcgcgc cgtggtgctg
     2701 gcggccgagg gcccggcctt ctgcgccggc gccaacctga actggatgcg ccgcatggcg
     2761 gactacacgc gcgagcagaa cctggcggac gccgccaagc tcgccgagat gctgcgcgtg
     2821 atctacgagt gcccgaagcc gacgattgcg cgcgtgcagg gcgatgtgta cgccggcggc
     2881 actggcctgg tggccgcctg cgacatggcc gtcagcgtgg acactgcggg cttctgcctg
     2941 tccgaggtca agataggcct gatccccgcc accatcagcc cctacgtgat ccgtgccatg
     3001 ggtgcgcgcg ccgcgcaccg ctacttcttg acggccgagc gcttcggcgc gcaggaggcg
     3061 ctgcgcatcg gctttgtgca cgaggtggtg acggcggagc agctcgacgc caaggtcgat
     3121 gagctcgtca aggcattgat cagcgccagc ccgaacgccg tgcgcgcctg caaaaagctg
     3181 gtgctggacg tcgccgaacg cgaaatcaac gccgccctga tcgccgccac ggtggagggc
     3241 attgccgaca tccgcgccag tgacgagggc cgcgagggcg tgcagtcctt cctgcagaag
     3301 cgcaagccca gctggctggc ctgatcgttg caatggacgc gctctggctc cagatcgtgc
     3361 aatggctcca tacggtgggc ctgcacatcg actcaggcac tgcccaggcg gtggccgagg
     3421 gggccgcgcg cgccacgcag cagctggaca tgcccggcct gctggccctg gccgcggccc
     3481 tgggctgggc cagcggcatg cgcctgtatg cggtggtgtt tctggtcggc ggcatgggcg
     3541 ccctgggctg gatcgcgctg ccgccggcgc tgacggtgct gcagcacccg gtggtgctga
     3601 tggccagcgg cttcatgctg ctggtcgaat tctttgccga caaggtgccg tggctggaca
     3661 gcgcctggga cgcggtgcat gccttcatcc gcgtgccggc gggtgcgctg ttggctgctg
     3721 gcgtgttcgg tgccgacaac gccagcatgg ccgtggcggc agggttgttg ggcggcacgc
     3781 tgtcggccac ggccctggcc accaagatga cggcgcgcgc ggcggcgaat acctcgcccg
     3841 agccgttttc caactggggc ctgtcgttcc tggaggacgg cctggtcgtc gccacggtct
     3901 ggctggccac gcagcacccg ctggccttcg gcgttgcgct ggtactggcg ctggccgtgt
     3961 cggcgctgct gctggtgatc ttgttcaagt ttctgcgcgc cgtgctgcgg cgtgtttctt
     4021 ccctgttttc tgcttctgcc aaggtggcct gaatgtttac aaaaatcctg attgcgaatc
     4081 gtggagagat cgcttgccgt gttgctgcga ccgccaaacg catgggcgtg aaaaccgtcg
     4141 ccgtgtattc cgacgccgac gcccaggcca agcatgtggc ggcctgcgac gaggccgtgc
     4201 acattggcgc cagcgcgccc aaggacagct atctgcgctg ggagcgcatc atcgaggcgg
     4261 cgcagcagac cggcgcccag gccatccacc ccggctacgg ttttttgagc gagaacgaag
     4321 acttcgccca ggcctgcgcc aaggccggtc tggtgttcat cggcccgccg gcgtcggcta
     4381 tttcagccat gggcctgaag gccgagtcca agcgtctgat ggccaaggcc agcgtgccgc
     4441 tggtgccggg ctaccagggc gaggaccaag acccgcaact gctgcagcgc gaggccgatg
     4501 ccatcggcta ccccgtgctc atcaaggcca gcgccggcgg cggcggcaag ggcatgcgcc
     4561 tggtcgagaa aagcgaggac tttgccgccg cgctggaatc ctgcaagcgt gaggccatca
     4621 acagctttgg caacgacgcc gtgctggtcg agaaatacgt gctgcgcccg cgccacatcg
     4681 agatccaggt gtttggcgat acgcacggca actgcgtcta tctgttcgag cgcgactgct
     4741 cggtgcagcg gcgtcaccag aaggtgctgg aagaagcccc cgcccccggc atgacgcccg
     4801 agctgcgcgc ccggatgggc caggcggccg tggccgcggc ccaggccgtg ggctatgtgg
     4861 gcgcgggcac ggtcgaattc atcgtcgagc agccgggcgg ctacgacgca cccgaggcca
     4921 tgaagttcta cttcatggag atgaacacgc gcctgcaggt cgagcacccg gtgaccgagg
     4981 cgattaccgg cgaagacctg gtggagtggc agctgcgcgt ggccagcggc cagcctttgc
     5041 ccaagcgcca ggacgagctg cgcatcatcg gccacgccat cgaggcgcgc atctgcgccg
     5101 agaacccgga caacaacttc ctgcccgcca ccgggcgcct gaatgtctat cgcaagccgg
     5161 tctgcaccag cttcgagaga agccaggtgc gcgtggacga cggcgtgcgc gagggcgacg
     5221 ccatcagccc cttctacgac agcatgatcg ccaagctcat cgtgcatggc gacacgcgcg
     5281 aacaagcgct ggcgcggctg gacgacgcac tggcccagac ccacatcgtc ggcctggcga
     5341 ccaatgtgca gttcttgcgc catgtggtgc aaagcggcgc cttcgcccag gcgcggctcg
     5401 ataccgcgct gatccagcgc gaggaggcgg tgctgttcca gcaggagaag gttggcctgc
     5461 ccctggccgc cgccgccgcc gtggccagcc agctgctgcg cgagcgcgcc gggcagggcg
     5521 ccaacccctt cagccgcacc gatggctggc gttcgctcac cgcctgcaag cgccgcttcg
     5581 agttcgactt tggcggccag cacgccaagg cctggctcag ctatgagcgc gatggcagct
     5641 accggctggc cgtgggcgag ggcgaaggcg cggtcagcgg cgcgctcgcc tttgccctcg
     5701 cccccggtgg cgccatggac ctgcaatacg ccggccagcg cacccaggcc ctggtctacg
     5761 cccagggcga aagcgatcat gttttcaccc cgcgcggcgc cacacgcatc gaggccctgg
     5821 atctgctcgc ccatgcgggt gagacggccg gcgagggcgg gcgcctgacg gcacccatgc
     5881 cgggcaaggt ggtgtcgttt gccgtccagg ccggcgacaa ggtcagcaag ggccaggccc
     5941 tggcggtgat ggaggccatg aagatggagc acaccatcgc cgccccgcag gacggcgtgg
     6001 tggccgagct gctctacgcg cccggcgacc aggtgacgga aggcgccgag ctgctcaggc
     6061 tttccgccgt ctgaacacgc gcgcggcgcc gtcgcggtac gctcgcaggc catgaaaatt
     6121 accttttgct gtaccgacac caaggccgag ccctggctcc agggcctggc cgccgccctg
     6181 ccgcaggccg acatcagcct ttggcaaccc ggtgcgcccc aggccgacta cgccgtggtc
     6241 tgggctccgc cgcagcagtt cattgacgag caggcgggcc tgaaggcctt gttcaacatc
     6301 ggcgccggcg tggatgcgct gctgaaacta cgtctgccgc cgggtctgcg cgtggtacgg
     6361 ctcgacgacg ccggcatggc cgtgcagatg gccgagtatg tgtgccacgc gctgatccgc
     6421 cacttccgtg aattcgacga ctacgaagcc agcgcccgcg ccggccgctg gagctaccgc
     6481 aagccgcgcg agcggcgcga ctttcccgtg ggcgtgatgg gcctgggcgt gctcggcgag
     6541 cgcgtggcgc gcgccgtcgc gcagttcgat tttccggtgc gcggctggag ccgcagcccg
     6601 aaacaggtgg cgggcgtgca atgctttcac ggcgcgcagg gtttcaatga ttttctggcc
     6661 gccagccgtg tgctggtgaa cctgctaccg ctcacgcccg agacccagga catcatgaac
     6721 cgggacacgc tcgcgcgtct gatgcccggc gcctatgtga tcaacgtggc gcgtggcgcg
     6781 catctggtcg atgacgacct gctggcgcta ctggatggcg gccatgtcgc cggcgccacg
     6841 ctggacgtgt tccgcaccga gccgctgccc gccgggcacc cgttctggag tcacccccgg
     6901 atcacgctca cgccccacac ctcggcgcgc acgctgcgcg acgagagcat tgcccagatc
     6961 gcgggcaaga tcgcggccct ggcacgcggc gaggccattg ccggcgtcgt gagcctggag
     7021 cgtggttatt gacccgcacc catttcctac ttcaagagac aagccatgcc ccatccctcc
     7081 cgagtccgca tcatcgacgt aggcccgcgc gacggcctgc agaacgaaaa gacccccgtg
     7141 cccgccgaca tcaagatcgg cctcgtgcag cgcctgcagg acgcgggcct gcaggaaatc
     7201 gaggtcacca gctatgtgtc acccaaatgg gtgccgcaga tggccgacaa ccatgcggtg
     7261 atgagcggca tggcgcgcaa ggccggcgtg cgctacagcg tgctcacgcc caacctgaag
     7321 ggctgggagg cggcggtgct tgataagccc gacgagatcg tcgtcttcgg cgccgccagc
     7381 gaggccttca gccagaagaa catcaactgc agcattgccg agagcatcga gcgcttcgcc
     7441 ccggtggtgg atgccgccct ggccgcaggc gtggccgtgc gcggcgccat gagctgcacc
     7501 gtgggctgcc cctacgaggg cgagatcgcc ccggagcgcg tggcctacct ggccggcctg
     7561 atgcgcggca taggcgtgca gcgcgtggac gtggccgaca ccataggcgt gggcacgccg
     7621 ctcaaggtgc agcgcgccat agaggccacg gcccagcatt tcggcatcga cgatatctcc
     7681 ggccatttcc acgacaccta cggccaggcc ctgtccaaca ccctggcggc gttggagctc
     7741 ggcgtgtgga actaccaatc ctccatcgcc ggcctgggcg gctgccccta cgccaagggt
     7801 gccaccggca acgtggctac cgaggatctg gtctacatgc tgcagggcat gggcattgag
     7861 acgggcatcg acctggacgc tctggtcgat gccgggcagt acatcagcga ttttctgggg
     7921 cgcaagccgc agtcgcgcgt ggcggtggcg ctgttgaaca agcgcgcggg gtgatgaaca
     7981 gcggaacgcc accgcttgca tgcaggcctg cccatgtata aagtaatgaa tccttacggt
     8041 aaggaattca ctccatgacg gctgtcgcca agatcaccag caagggccag accacgattc
     8101 cggccgatat ccgcgccgcc ctgcaggtcg gcccggggga caagctcgcc tgggaggtgt
     8161 tggaggcggg cgtggtacag gtgcgccgcg tacagccgct ggatatggag tttctccagg
     8221 ccatggaagg cacactggac gaatgggccg gcgccgccga cgaagaggcc taccgtgatc
     8281 tatgagcgct ggcaggtggt gcgcgtgcct tttcccttta ccgaccgcct tgccagcaag
     8341 aaccgcccgg cactggtgct ttccgccgcc gcggccttca acgccccggc tgggcatctg
     8401 gtgctggcca tgatcacgtc cgccggcaat gccccgtggc cgctggactg tgccatccag
     8461 gatcttgccg gtgctggttt gccggcgccg tccgtggtgc gctgcaagct attcaccctg
     8521 gatgcgcgcc tggttcgcgg cagcctcggg cgcctggcgc cccacgatgt gcaggagatc
     8581 gagcacagtc ttggccgcct gttcggcgct gccgtctgac gctcctgatg caacctattt
     8641 gcttatgaat cccgctgccg tttcccctct gcccgaagcc gtacaacgcg tagccgctgc
     8701 cttgcaggcg gcccgccacc cgcatatgcc gatcatgctg gacgacgccg cgcgcacggc
     8761 gcagcaggcg gccgacgcct tgggcgtgca gcttgggcag atcgccaaga gcatcatctt
     8821 ccggcgcaag agcgacgacg cggcggtgct ggtcatcacc tcgggcgacc ggcgcgtgga
     8881 tgagaaaaag gtggatgccc tggtcggcaa gacgggccgg gccgacgccg agttcgtcaa
     8941 ggctaagacc ggcttttcca tcggtggtgt ttcgccgctg gcgcacgcgc aggcgccggt
     9001 cacgctgatc gaccgcgagc tgctgcgctt cgaggtgatc tgggccgcgg ccggccatcc
     9061 gcatggcgtg ttccagctgc ggccgcagga tctgcaggcc ttcaccggcg cgccgctggc
     9121 cgatgtggtg caggtagtcg tgtcatgaaa ctatcaacgt tgatagctgc tcgcgcccaa
     9181 cagataagcg ttacgggcca atttcattca aattacacgg gagtgatccc ctcgccctgc
     9241 ctgtccatct gtcgcatgac gcgcgacggc agccactgcg agggctgttt tcgcaccctc
     9301 gatgagatcc gcacctggtc gcaggcgggc gctgcccggc gccgggccat ctgggcagac
     9361 ttgctgcggc gtgccggggt tgcattgccc gaggaactgg catgaaagag atcacctgtt
     9421 acctggactt cgtctccccc tacgcctggt tggccttcga gcgcctgccc caggcgctgg
     9481 aggggctgtc ctaccgcctg agctaccgtc cggtgctgct gggcgcgctg ctgaacgccc
     9541 atggcaatcc cgggccggcc ggcattgtcc ccaagcgcga ctggacctac cgccatgtca
     9601 gctggctggg ccacagcctg ggtgtgggcc tggacatgcc ggcgcgccac ccgttcccgc
     9661 cgctgccgct gttgcgcctg gcgctggagt gcagcgacga tggcctggtg aaccgctttg
     9721 tcgccggcag catcctgcgc catgtctggc agggcggggc ggacgcgctc gacccggcgc
     9781 gcctggacgc gctgcgcgcc ctgctggccg agcagctgcg cccggacgag gccgcgcccg
     9841 aacgcgccaa gcagctgctg cgcgccaaca ccgagctggc ggcgcagcgt ggcgtgttcg
     9901 gcgtgcccat gttcgaggtc gatggccatt tgttctgggg cctggacagc ctgcccatgc
     9961 tgcgtgccta tctcgatggg gatggctggt ttgcgcaaca ctggcagcgc tgtgcaagcg
    10021 tgccgcaagg cttgccgggt tgaattgggc tcatgcccaa tgccttcaac ttctccgcct
    10081 cgccgttcga ctgcctgacg gccgaagaac agtcgctggt gcgcgacaac gtggacattg
    10141 cctactaccc cgaaggggcg gtggtgctgg atgtgggcaa tgcgcccgag catctgttcg
    10201 tcatcatcaa gggctatgtc acgcagaccg aaggcgagga ggtgctggct acctacgggc
    10261 cagaagactg ttttgacggc cgcggcctgg tggccgggcg cgtcagcagc cgcttcaccg
    10321 tggccgagga gctggtggcc taccagttgg cgcgcagcac cgtcaaggat ctgatcgcgc
    10381 gcaaccgcgg ctttggcgcg ctgctctttt ccgacctggg caacaagctc tcggccctgg
    10441 cgcagcgcgc cgaacagcac cagatgcagt cgctgacgct ggcgcgggtc gatcaggcct
    10501 ttctgcgccc ggcgcatgtg gtggccgcgg gcaccgacat cgtctccgtg gtgcgcatct
    10561 ttcaggatca gcgcaccacc agcgtgctgg tcagcggcct gcccgatggg ctggggattt
    10621 tttccaacac cacgctgcag cgtgccatcc tcgatggccg gcccttggag cgcatcaccg
    10681 tgggcgagct ggccagcagc ccggtggtca cggtgcgtgc tgccgagcag attggcgacg
    10741 ccatggcgtt gctcttgcgc gcgcgcgtgc accgcctggc ggtgctgggc gaggacggcc
    10801 aggtactggg catcctggag gcgctggacc tgttcagcta cctggccaac cactcgcacc
    10861 tgatcacggt gcagatcgag gaggcgcgcg acctgccggc gctggaggcc gccgccgcgc
    10921 aaatcacccg cctggtggcc gccctgcacc gcggcggcac gcgcatcgcc ctggtggcgc
    10981 agctggtgca gcagctcaac gcgcgcctgt tcgagcgcgc ctggcagatg attgccccgc
    11041 ccgatctgct ggccaacagc tgtttgttcg tcatgggcag cgaggggcgc ggtgagcagc
    11101 tcttgaagac cgaccaggac aacggcctgc tgctgagtga cggctacgag ccgccggccg
    11161 atctgcaggc catttgcgac cgcttctccc aggccctggc cggttttggc taccccgaat
    11221 gcgccgggcg catcatgctc aacaaccccg agtggcgcgg cacggtgacc ggctttggcg
    11281 acaaggtgcg cggctggctg ctgcagcccc agggcgagag cctgatgtac ctggccatct
    11341 tcatggacgc ccacgccgtc gctggcaagg cccagctgct ggccgaggtg cgccagcgcg
    11401 tgatgcagct ggccaccgac aacgatgcat tggtggcgcg ctttgccatg gccatagacg
    11461 cctttggcgg cgccgccggc tggtggaacc gcctgctcag cctgggcggc gacgccgacc
    11521 tggtgaacct caagaaagcc ggcatcttcc ccatcgtgca tggcgtgcgc agcctggcgc
    11581 tggcgcggcg catcaaggcc accggcaccg tcgagcgcat tgccgcactg cagggcgaag
    11641 gccagctgga cgaggccatg gcgcaggaac tggtgcaagg cctgcacttt ctgatgggcc
    11701 tgcgcctgca ggccggcctg gccgagctgg agctgcagcg gccggtgacc ggcaacgtcg
    11761 atccgcagcg cctgtccagc ctggagcgcg atctgctcaa ggacacgctc tcggccgtca
    11821 aacgcttcaa ggccctgctg caccagcgcc tgcgcctgga cgccgtatga gccgccacta
    11881 cagccaggcc gccccgctgt ggtgggaaaa gctgcgccgc tggtggctga tctatcacct
    11941 gggcgagccc gagttcgcgc ccatgttcga cccgccgccg cacgatgaat gggtggcgct
    12001 cgactgcgag accaccgggc tgaacacgcg cagcgacgag atcatctccg tcggcgcggt
    12061 gcgcatcgtc ggcgaccgca tcatgaccag cgagcgcctg gagctgttga tacgccccga
    12121 gaaaggcgtg tccgccgaca gcgtgcgcat ccaccgcctg cgcgagcgcg acctggaggg
    12181 cggcctgccg aaggacgagg ccatgcgccg gctgatgcgc ttcataggca gccgcccgct
    12241 ggtgggctac tacctggagt tcgacgtggc catgctcaac cgcgccatct ggccgctgct
    12301 gggcatgggt ctgccgcagc ccaagatcga ggtctcggcc atgtactacg actacaagtt
    12361 caagcagctg ccgccctacc agcagcaggc gaccaccgag atcgacctgc gctttgccac
    12421 cctgatgcag gatctggccc tgcccgagcg cgaggcacat gacgcgctca atgacgcagt
    12481 gatggccgcc atggccttca tcaagctgcg ccgcttgcgc ggggaagcat aagcaaaaat
    12541 tggcataaac cgcctgtcta tcggcgcaat aagctattct aaagatagca aaaaaccggg
    12601 cccttgtggg gcccggtttc gttgatgggg gcttcgcagc ccctccctcc ttggaaatcg
    12661 atcagtggcc ggatgcgccg gaagcgccca ggccggtttc cgagcgcacc tgctgcgccg
    12721 ggaaggctgc gcgatccttg gccgcgcggg cgctgctgtc ggtgatcgag aacagccaca
    12781 cgccgacgaa gccgatggtc atggagaaca gcgccggcga cgagtagggg aacagggccg
    12841 aaccggccgg gttgcccagc gtggcttccc acaccgaggg cgagacgacg gtcagaccca
    12901 ccgacgagat cagccccagg aagccgccga acaccgcgcc gcgcgtggtg cagcccttcc
    12961 acaggatgga caggatcagc gccgggaagt tggccgaggc ggcgatagcg aaggccagcg
    13021 acaccatgaa ggcgatgttc tgcttctcga aggcaatgcc cagaatcacc gcgacgacgc
    13081 ccagagccag ggtggtcatg cgcgagactt tcagttcggc cgcgctgtcg gccttgccct
    13141 tcttgatgac cgtggagtac aggtcatgcg acacggccga ggcgcccgac agcgtcaggc
    13201 cggccaccac ggccaggatg gtggcgaagg ccacggccga gatgaagccg tagaagatgt
    13261 tgccgcccac ggccttggcc accagcaccg ccgccatgtt ggccgtgccc gcgccgccct
    13321 tgatcacgcc cttgaccgtg tcggccatct cggggttcgt cagcaccatg gtgatggcgc
    13381 caaagccgat gatgaagatc aggatgtaga agtagccgat ccacaccgtg gcccagccca
    13441 ccgacttgcg
//
LOCUS       DAOMTQ010000084        13356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_799_length_13356_cov_29.766935, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000084 DAOMTQ010000000
VERSION     DAOMTQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_799_length_13356_cov_29.766935"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..414)
                     /locus_tag="PK518_09360"
     CDS             complement(136..414)
                     /locus_tag="PK518_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRM49163.1"
                     /translation="MKCPQCNDVTLVMAERQGVEIDYCTQCRGVWLDRGELDKLIERS
                     AHASPAAAPQPAYQRPTHRPDFEDSDFRHHGSHGQQRKHKSWLSEIFD"
     gene            complement(431..1108)
                     /locus_tag="PK518_09365"
     CDS             complement(431..1108)
                     /locus_tag="PK518_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRM49164.1"
                     /translation="MNRPLALSTPSASTGWGASIARHRVLRNTYALLSMTLLFSAAIA
                     GLAVALQAPPAGLVITLVGYFGLLFLVHKLQHSGWAIPVVFALTGFMGYTLGPVLAKH
                     LALPGGAQTIALALGATGATFLALSAWVLASRRDFSFMGGFLFAGMVIALLAGLGAVF
                     LQLPALSLAVSAMVALLSAGMILFETSRIVNGGETNYVLATVGLFVSLFNLFTSLLSL
                     FGFGSND"
     gene            1207..2088
                     /locus_tag="PK518_09370"
     CDS             1207..2088
                     /locus_tag="PK518_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49165.1"
                     /translation="MSLAFNYRHLYYFWVVAKEGGMSRAATRLDMAIQTVSAQVRELE
                     RDLGCQLLKPAGRGLALTEAGVVALQQAEQIFQLGESLPELVRGAAQVQAVRLTVGIA
                     DGLPKLVVQRLLQPVLATPGLRLVCHEGEMADLLADLALHRMDVVLSDHPAPFNPHLK
                     VHNHPLGASAMGWYAAPYWWEQAQDGFPASLNRVPVLLPTTHSTVRRPLDQWLIQHRL
                     RPRVVGEFEDSALLETFGGSGLGVFPAALSVQDDLLQRHQARLLGPCEGVEDHYYAIS
                     TERRVKHPVVQQLLARG"
     gene            complement(2110..2910)
                     /locus_tag="PK518_09375"
     CDS             complement(2110..2910)
                     /locus_tag="PK518_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRM49166.1"
                     /translation="MFKLTSLNLNGIRSATSKGVEEWMAQNRPDCICVQELKAQSADM
                     QGRFEELAGLSGHFHFAEKKGYSGVGIYTRHAPSDVIIGYGSDEFDAEGRYVELRFDR
                     PGRRLSLISAYFPSGSSGEERQQAKFRFLDEFHPHLMRLKAEREFILCGDINIAHQQA
                     DLKNWRSNQKNSGFLPEERAWMTKLLGAEPDGGLVDVYRRLQPETTDACYTWWSNRGQ
                     AYANNVGWRLDYHLATPQLAELARSEAIYKGEKFSDHAPITVGYDLVL"
     gene            2921..3619
                     /gene="pyrE"
                     /locus_tag="PK518_09380"
     CDS             2921..3619
                     /gene="pyrE"
                     /locus_tag="PK518_09380"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRM49167.1"
                     /translation="MVTDNAPAPYQDALAQEFVRFAVESGVLRFGEFKTKAGRMSPYF
                     FNAGLFDDGAKLGRLAQFYAQALLASGVEFDMVFGPAYKGIPLAATVAVELARLGRNV
                     PFAYNRKEAKDHGEGGTLVGAPLAGRVLIIDDVMSAGTAARESIALIQAAGATPHAMA
                     IALDRQEKATENGQDVEHSAVQYVRRELGMQVCAIAKLPDLLQYLAADGGDAQMHAHH
                     ERVLAYRQRYGVND"
     gene            3677..4333
                     /locus_tag="PK518_09385"
     CDS             3677..4333
                     /locus_tag="PK518_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRM49168.1"
                     /translation="MACVALAAAAQAWAQQARPPQEVYTCIDKHGRRITSDRPIADCV
                     DREQRVLDHTGTERRRIGPSLTEHERAAQEVQRRKQAEESARIAEERRRERALTARYP
                     DEATHQAERNAALDQVEEVVVVAHKRVQSLKAERRRLDVELEFYRGDVTKAPVLLQRQ
                     IADNEQALADQQRFLATQELEKRRIHQRFDAELAQLRQLWAAQRASSTQWGQLPAPQK
                     "
     gene            complement(4391..5836)
                     /gene="gatB"
                     /locus_tag="PK518_09390"
     CDS             complement(4391..5836)
                     /gene="gatB"
                     /locus_tag="PK518_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRM49169.1"
                     /translation="MSTQLIQGYEVVIGFETHAQLATQSKIFSRASTAFGAEPNTQAS
                     AVDLALPGTLPVMNREAVACAIKLGLALGSHIAPESIFARKNYFYPDLPKGYQISQFE
                     IPVVQGGEVSFFLGEEKKTVRLVRAHLEEDAGKSLHEEFHGMSGIDLNRAGTPLLEIV
                     TEPDMRGSEEAVAYAKALHQIVTWIGICDGNMQEGSFRCDANVSVRKPGAPLGTRREI
                     KNLNSFRNMQQAIDYEIRWQIEQLEDGHKIQQATVLFNPDTGETRAMRTKEDAADYRY
                     FPDPDLPPLRIAASWIDEVKAAMPELPRAMAARFVAQYGLPEYDATTLTQSRAMAAYF
                     EQAAQACGQAKLASNWIMGEISRRLNAEEIGMEAVKVTSSQLATLISRIQDGTISNNA
                     ARQVFEALWNGEATEVDAIIEAKGLKQMNDSGALEKIIDEVIAANPANVEQYRAGKDK
                     AFNALVGQVMKASKGKANPQQVNELLKAKLA"
     gene            complement(5839..7332)
                     /gene="gatA"
                     /locus_tag="PK518_09395"
     CDS             complement(5839..7332)
                     /gene="gatA"
                     /locus_tag="PK518_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRM49170.1"
                     /translation="MNNNTDLHDLGVAQLAAALRAKQVSAVEAAQHFLARAKAHQGLA
                     AYLAIDEDVTLAQARAQDAALATGQAAPLAGLPIAHKDVFVTRDFPTTAASKMLAGYR
                     SPFDATVVARLAQAGCVTLGKLSCDEFAMGASNEKAAIAALGLDAPQPAHNPWNPRHV
                     PGGSSGGSAVAVAARLAPAATGTDTGGSIRQPASFCGVTGIKPTYGRASRYGMIAFAS
                     SLDQAGPMARSAEDCALLLSQICGPDPERDATSLDHPAEDFTRGLQGSIAGLRIGVPA
                     EFFSDGLQDDVRTAVEAALKEYEKLGARLVPISLPRTALSVPVYYILAPAEASSNLSR
                     FDGVRYGHRAENYSDLLDMYKKTRAEGFGDEVKRRIMIGTYVLSEGYYDAYYLQAQKI
                     RRMIADDFQAAFQQCDLIAGPVAPSVAPQLGAQTDPVSSYLADIYTLPASLAGLPGMS
                     VPAGFGGAGLPVGLQLIGNYFQEGRLLNAAHQLQLATDHHLRRPEGF"
     gene            complement(7343..7642)
                     /gene="gatC"
                     /locus_tag="PK518_09400"
     CDS             complement(7343..7642)
                     /gene="gatC"
                     /locus_tag="PK518_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRM49171.1"
                     /translation="MALTPQDIGRIAHLARLELAPQESERMLTQLNDFFSIVEAMQAV
                     DTSGVTPLPHPAAVIQDVHLRLAADVASEPNQREANQRSAPAVERGLFLVPRVIE"
     gene            7860..8903
                     /locus_tag="PK518_09405"
     CDS             7860..8903
                     /locus_tag="PK518_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRM49172.1"
                     /translation="MFGAFRRYFSTDLAIDLGTANTLIFARDKGIVLDEPSVVAIRHE
                     GGPHGKKVIQAVGREAKAMLGKVPGNIEAIRPMKDGVIADFVITEQMIKQFIKMVHPR
                     SVLTPSPRIIICVPCGSTQVERRAIKDAAEAAGATAVYLIEEPMAAGIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSVRVGGDKFDESIINYIRRNYGMLIGEPTAES
                     IKKHIGSAFPGSEVKEMEVKGRNLSEGVPRSFTISSNEVLEALTEPLNQIVSAVKTAL
                     EQTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPVLVAEEPLTCVVRGCGIAL
                     ERMDRQGSIFTSE"
     gene            8933..9850
                     /gene="mreC"
                     /locus_tag="PK518_09410"
     CDS             8933..9850
                     /gene="mreC"
                     /locus_tag="PK518_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRM49173.1"
                     /translation="MPLGTLDRSAPTLFKHGPSPLSRLALYSALALFLMVADARFRIT
                     EPLRKVVATALYPLQWLMLQPVELADKGLGYLQSLQAAQQAADAAQKQMAQMSVRAGE
                     TDQLLRENAELRQLLALRARLDTPAQAAQVIYDTADPYTRRVMVDRGQLAGVQAGAPV
                     VDASGVLGQVTRVYPMLSEVTLLIDRDQAIPVLNVRTGARSVAYGDPVAGLGGGMELR
                     FTPGNADVQEGDLLTTSGVDGLYPPGLPVARVVRVERRADSTFARIYCAPLAQVYGAR
                     HVMLLEPLANDLPPRPAPEPATQKRGGHK"
     gene            9847..10368
                     /gene="mreD"
                     /locus_tag="PK518_09415"
     CDS             9847..10368
                     /gene="mreD"
                     /locus_tag="PK518_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRM49174.1"
                     /translation="MIMPKGQQLLLPVNPVFIVASLVLALALNLLPLGRVVWTPDWVM
                     VLLVFWGIHQPQRVGLGVAFAMGLCIDVAQSALLGQHALVYCGVMFGAQLAHRRLLWF
                     GPLWQALQLLPLFFAGHAVELALRVVAGGGTLPGLEGLAAPLLEAVIWPLASWVLLAP
                     QRRPPDQDDNRPL"
     gene            10388..12331
                     /gene="mrdA"
                     /locus_tag="PK518_09420"
     CDS             10388..12331
                     /gene="mrdA"
                     /locus_tag="PK518_09420"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRM49175.1"
                     /translation="MTELRNVEADARRFRLRVFVLGVVVLLAFLLIVARLLVLQVERH
                     DDLAEQAESNRTAIVPIVPNRGLIMDRNGVVLATNYSAYTLEITPSRAIDLEGTIDQL
                     AQIVDIQQRDRRRFKRLREESRSFDSLPIRTRLSDEEVARFAAQRYRFPGVEIKARLF
                     RTYPMGEVASHAIGYIGRINQREKERIEDSDDAANYRGTDYIGKLGVEQSFEPTLHGQ
                     TGFERMETSAGGHAVRRLNSHPATPGNTVMLSLDIKLQKLIEEMFGERRGALVALDPR
                     NGEVLALVSKPTFDPNLFVEGIDQENWQALNESINKPLLNRALRGTYPPGSTYKPFMA
                     LAALELKKRSATQVISDPGFYNYGGRTFRSHEGGLGGVDMHRAIQYSSNTYFYSLGVE
                     MGVDAIHDFMKPLSFGQITGIDLGGEVRGVLPSMEWKRNTYKRPEMKRWYSGETVSLG
                     IGQGYNNFTMLQLAVAEATLANGGTRHRPHLIKAVKDQVTGQVTEVQQPPGENLGYNP
                     KNVAIINNALMAVNQAGTGRRVFLGAPYTSAGKTGTAQAVAWGQNTKYNARLLEEHKR
                     DHSLFAAFAPVEDPKVAVAVIVENAGFGAAAAAPIVRRVFDYWLLGQYPSEQDLAAVS
                     KGQAAAPIGVPRRADDVPLTPEP"
     gene            complement(12332..13222)
                     /locus_tag="PK518_09425"
     CDS             complement(12332..13222)
                     /locus_tag="PK518_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM49176.1"
                     /translation="MRSERQGLMALLIVVAVWGTTFPTMKLLSAQLDALQIIWARFAI
                     ALLVLLPMWRGMRRTELHWGLLLGALLFIAFWLQTEGLARTSSNRNAFVTGLNVLVVP
                     LLAMLALGRRYGLALWAACAMALVGMTLMFHEDAPWNLGDTLTLASTLFYAVYILGLE
                     ECARRTASAPLRATRMAASQALVMCVASGALMLARHGEFAGTWDAIATLPGPALTALA
                     YLGVIASVLVVTLQAWGQQRVDAMRSAIVFGLEPVFAAATAWFLIGERLGAVGLVGAA
                     LIVAALIISQLQPPRAATLA"
BASE COUNT         2170 a         4355 c         4644 g         2187 t
ORIGIN      
        1 cccaggcgct tggcatgggc cagcgagggg gcggccaggg cggctgcggt cagggcgatc
       61 acggtagcgg tggagcgaaa agaagcgttc atggagtttt cctgagaaga atggcaaggg
      121 tgggggagag gcggcttaat cgaagatctc gctgagccag gacttgtgct tgcgctgctg
      181 gccgtgcgag ccgtggtggc gaaagtccga gtcctcgaag tcgggccggt gcgtggggcg
      241 ctggtaggct ggctgcggcg ccgctgccgg gctggcgtgg gcgctgcgct cgatcagctt
      301 gtccagctcg ccacggtcca gccagacgcc acggcattgc gtgcagtagt cgatctccac
      361 gccctggcgc tcggccatga ccagggtgac gtcgttgcac tgggggcatt tcataggctt
      421 ttccttgtga tcagtcgttg ctgccaaagc cgaacaggct cagcaggctg gtgaacaggt
      481 tgaacagcga cacgaacagg cccaccgtgg ccagcacgta gttggtctcg ccgccgttca
      541 cgatgcggct ggtctcgaac aaaatcatgc cggccgacag cagtgccacc atggccgaga
      601 cggccagcga cagcgccggc agctgcagaa acacggcgcc caggccggcc agcagggcaa
      661 tcaccatgcc ggcgaagaga aagccgccca tgaagctgaa gtcgcgccgc gaggccagca
      721 cccaggccga cagggccaga aaggtcgcgc ccgtcgcgcc cagggccagc gcaatcgtct
      781 gcgcgccgcc gggcagcgcc agatgtttgg ccagcaccgg gcccagggtg tagcccatga
      841 agcccgtcag ggcgaacacc acgggaatcg cccagccgct gtgctgcagc ttgtgcacta
      901 gaaacagcag gccaaagtag ccgaccaggg tgatgaccag gccggccggc ggcgcctgca
      961 gcgccaccgc cagccccgca atggccgcgc tgaacagcag cgtcatggac agcagggcat
     1021 aggtgttgcg cagcacccgg tgccgggcaa tgctggcgcc ccagccggtg ctggcgctgg
     1081 gtgttgagag agcaagaggt cggttcatgg tgaatcccgg gtaacggaga agggcgccag
     1141 aatagggtgt tgataccttg cgaaaaagca gacaatcagt gattgaaaat cctgtaaaaa
     1201 ctgaatatga gcctggcctt caactaccgc catctgtact acttctgggt ggtcgccaag
     1261 gagggcggca tgtcgcgcgc cgccacgcgc ctggacatgg ccatccagac ggtgagcgcc
     1321 caggtgcgcg agttggagcg tgatctgggc tgccagctgc tcaagcccgc cggccgtggc
     1381 ctggcactga ccgaggccgg ggtggtggcg ctgcagcagg ccgagcagat cttccagctc
     1441 ggtgaatcgc tgccggagct ggtgcgcggc gcagcccagg tgcaggccgt gcgcctgacg
     1501 gtggggattg ccgacggcct gcccaagctg gtggtgcagc gcctgctgca gccggtgctg
     1561 gccacgcccg gcctgcggct ggtgtgtcac gagggcgaga tggcggacct gctggccgac
     1621 ctggcgctgc accggatgga cgtggtgctg tccgaccacc cggcgccctt caacccgcac
     1681 ctgaaggtgc acaaccaccc cctgggcgca tcggccatgg gctggtacgc ggcgccgtac
     1741 tggtgggagc aggcgcagga cgggtttccg gcctcgctca accgggtgcc ggtgctgctg
     1801 cccaccaccc attccaccgt gcgccgcccg ctcgaccagt ggctgatcca gcaccggctg
     1861 cgcccgcgcg tggtcggcga gtttgaagac agcgccctgc tggagacctt tggcggcagc
     1921 ggcctggggg tgttcccggc cgcgctgtcg gtgcaggacg atttgctgca gcgccaccag
     1981 gcccggctgc tcggcccctg cgagggggtg gaagaccact actacgccat cagcaccgag
     2041 cgccgcgtca agcacccggt ggtgcagcag ctgctggccc ggggctgagc cgcgcgccgg
     2101 ctcgatgcgt cacaagacca ggtcgtagcc gacggtgatc ggcgcatggt cgctgaattt
     2161 ctcgcccttg tagatcgcct cgctgcgcgc cagttcggca agctgcggcg tggccaggtg
     2221 gtagtcgagg cgccacccca cattgttcgc ataggcctgg ccgcggttgc tccaccatgt
     2281 gtagcaggcg tcggtggtct cgggctgcag gcggcggtac acgtccacca ggccgccatc
     2341 gggttcggcg cccagcagct tcgtcatcca ggcgcgctct tcgggcagga agccgctgtt
     2401 cttctggttg ctgcgccagt tcttcaggtc cgcctgctgg tgtgcgatgt tgatgtcgcc
     2461 gcagaggatg aactcgcgct cggccttcag ccgcatcagg tgcggatgga attcgtcgag
     2521 gaagcggaac ttggcctgct ggcgctcctc gcccgagctg ccgctgggaa agtaggcgct
     2581 gatcaaggac agcctgcgcc cgggtcggtc aaagcgcagc tccacatagc ggccttcggc
     2641 gtcgaattcg tcggagccat agccgatgat cacgtcgctg ggcgcgtggc gtgtgtagat
     2701 gcccacgccc gaatagccct tcttctcggc aaagtgaaaa tggcccgaca ggcccgccag
     2761 ctcttcaaaa cgcccctgca tgtcggcgga ctgggccttg agctcctgca cgcaaataca
     2821 atccgggcgg ttttgcgcca tccattcctc cacgcccttg gacgtggccg agcggatgcc
     2881 gttgaggttg agactggtta atttgaacaa ggatgcatcc atggtgacag acaacgcgcc
     2941 ggcgccgtat caggacgccc tggcacagga attcgtgcgt tttgcggtgg aatccggcgt
     3001 gctgcgcttt ggtgagttca agaccaaggc cgggcgcatg agtccatatt tcttcaacgc
     3061 cggcctgttt gacgatggcg ccaagcttgg gcgcctggcg caattctatg cacaggcgct
     3121 gctggccagc ggcgtggagt tcgacatggt cttcggcccg gcctacaagg gcatcccgct
     3181 ggccgccacg gtggccgtgg agctggcgcg gctgggcagg aacgtaccct ttgcctataa
     3241 ccgcaaggag gccaaggacc atggcgaggg cggcaccctg gtgggcgcgc cgctggcggg
     3301 gcgcgtgctc atcatcgacg acgtgatgag cgccggcacg gccgcgcgag agtccatcgc
     3361 cctgatccag gccgccggcg ccacgccgca tgccatggcg attgccctgg atcgccagga
     3421 gaaggccacc gagaacggcc aggacgtgga gcacagcgcc gtgcaatatg tgcgccgcga
     3481 gctgggtatg caggtttgcg ccatcgccaa gctgcccgat ctgctgcaat acttggcggc
     3541 tgacggtggc gacgcgcaga tgcacgccca ccacgagcgc gtgttggcct atcgccagcg
     3601 ctatggcgtc aatgactgag gcatgggccg tgagaggggc aatggcttga aaacaaaggc
     3661 agtgacggga gggctgatgg catgcgttgc gctggccgcg gcagcgcagg cctgggcgca
     3721 gcaggccagg ccgccgcaag aggtctacac ctgcatcgac aagcatggcc ggcgcatcac
     3781 ctccgaccgg ccgattgccg attgcgtgga tcgtgagcag cgcgtgctgg accacaccgg
     3841 caccgagcgc cgccgcatcg gcccctcgct gaccgagcat gaacgcgcgg cgcaggaggt
     3901 gcagcgccgc aagcaggccg aggagagcgc ccgcatcgcc gaggagcgcc ggcgcgagcg
     3961 cgcgctcacg gcgcgctacc ccgacgaggc cacgcaccag gccgagcgca atgcggcgct
     4021 ggatcaggtg gaagaggttg tcgtcgtggc gcacaaacgc gtgcagtcgc tgaaggccga
     4081 acgcaggcgg ctggacgtgg agctggagtt ctaccgcggc gacgtgacca aggcccccgt
     4141 actgctgcag cgccagattg ccgacaacga gcaggccctg gccgaccagc agcgctttct
     4201 ggccacccag gagctggaga agcgccgcat ccaccaacgc ttcgatgccg agctggcgca
     4261 gctgcgccag ctgtgggcgg cgcagcgcgc ctcgtccacc caatgggggc agctgccggc
     4321 gccgcaaaaa taaaggctcc tgcaggagcc tttatttttg atagctgttt gcgcttgtct
     4381 ggcgggcgtt tcaggccaat ttggccttta acagctcatt gacctgctgc ggattcgcct
     4441 tgcccttgct ggccttcatc acctggccga ccagggcgtt gaaggccttg tccttgccgg
     4501 cgcggtactg ctccacgttg gccgggttgg cggcgatcac ctcgtcgatg attttctcca
     4561 gcgcgccgct gtcattcatt tgcttcaggc ccttggcctc gatgatggcg tccacctcgg
     4621 tggcctcgcc gttccacagc gcctcgaaga cctgcctggc cgcgttgttg ctgatggtgc
     4681 cgtcctgtat gcggctgatc aacgtggcca gctggctgct ggtgaccttg actgcctcca
     4741 tgccgatttc ctcggcgttc aggcgccgcg agatctcgcc catgatccag ttgctggcta
     4801 attttgcctg gccgcaggcc tgggctgcct gttcgaagta ggccgccatg gcgcggcttt
     4861 gcgtgagtgt ggtggcgtcg tattcgggca ggccgtattg cgccacgaag cgcgccgcca
     4921 tggcgcgcgg cagctcgggc atggcagcct tgacctcgtc tatccagctg gcggcgatgc
     4981 gcagcggtgg cagatccgga tcggggaagt agcggtagtc ggccgcgtcc tccttggtgc
     5041 gcatggcgcg cgtctcgccg gtgtcggggt tgaacagcac ggtggcctgc tggattttgt
     5101 ggccgtcctc cagttgttcg atctgccagc ggatctcgta gtcgatcgcc tgctgcatgt
     5161 tcctgaagct gttcaggttc ttgatctcgc ggcgcgtgcc cagcggcgcg ccgggcttgc
     5221 gcacgctgac gttggcgtcg cagcggaacg acccctcctg catgttgccg tcgcaaatgc
     5281 caatccaggt gacgatctgg tgcagcgcct tggcgtaggc cacggcttct tcgctgccgc
     5341 gcatgtcggg ctcggtgacg atctccaaca gcggcgtgcc ggcgcggttc agatcgatgc
     5401 cgctcatgcc gtggaactct tcgtgcaggc tcttgccggc gtcttcctcc aggtgggcgc
     5461 gcaccaggcg cacggtcttc ttttcctcgc caagaaagaa cgacacctcg ccgccctgca
     5521 ccaccgggat ctcgaactgg ctgatctggt agcccttggg caggtccggg taaaagtagt
     5581 tcttgcgggc aaaaatgctc tctggcgcaa tgtgggagcc aagagccagt cccaatttga
     5641 tagcgcaggc gacggcctcg cggttcatca ccggcagggt gccgggcagg gccagatcga
     5701 cggcgctggc ctgggtgttg ggctcggcgc caaaggcggt gctggcgcgg ctgaagatct
     5761 tggattgcgt ggccagctgg gcgtgcgtct cgaagccgat gacgacctcg tagccctgga
     5821 tcagttgcgt gctcatggtc agaagccctc cgggcggcgc aggtggtggt cggtggccag
     5881 ctgcagctgg tgcgcggcat tgagcaaacg accctcctgg aagtagttgc ctatcagctg
     5941 caggcccacg ggcaggcccg cgccgccaaa gcccgccggc acgctcatgc cgggcaggcc
     6001 ggccagcgac gccggcaggg tgtagatgtc ggccaggtag ctgctcaccg ggtcggtctg
     6061 cgcgcccagc tgcggcgcca cgctgggcgc caccgggccg gcgatcaggt cgcattgctg
     6121 gaacgcggcc tggaagtcgt cggcgatcat gcggcggatc ttttgcgcct gcaggtagta
     6181 ggcgtcgtag tagccctcgg acagcacata ggtgccgatc atgatgcggc gcttgacctc
     6241 gtcgccaaag ccttcggcgc gggtcttctt gtacatgtcc agcaggtcgc tgtagttctc
     6301 ggcgcggtgg ccatagcgca cgccgtcaaa gcgcgacagg ttggagctgg cctcggccgg
     6361 ggccaggatg tagtacaccg gcaccgacag cgcggtgcgc ggcaggctga tgggcaccag
     6421 ccgggcgccc agcttttcgt attccttcag ggccgcctcg actgcggtgc gcacgtcgtc
     6481 ctgcaggcca tcgctgaaaa attctgccgg cacgcctatg cgcaggccgg cgatggagcc
     6541 ctgcaggccg cgcgtgaaat cctcggccgg gtgatccagg ctggtggcgt cgcgctccgg
     6601 gtcggggccg cagatctggc tcagcagcag ggcgcagtcc tcggcgctgc gtgccatggg
     6661 gccggcctga tccaggctgg aggcaaaggc gatcatgccg taacggctgg cgcggccgta
     6721 ggtgggtttg atgccggtga cgccgcaaaa gctggccggc tggcggatgg agccgcccgt
     6781 gtcggtgccg gtggccgccg gtgccaggcg cgccgccacg gccacggccg agccgccgga
     6841 tgagccgccg ggcacatgcc gcgggttcca ggggttatgc gcgggctggg gcgcgtccag
     6901 cccaagggcg gcaatggcgg ccttttcgtt gctggcgccc atggcgaact cgtcgcagct
     6961 gagcttgccc agcgtcacgc agcctgcctg ggccagacgc gcgaccaccg tggcatcaaa
     7021 cggcgagcgg tagccggcca gcatcttgct ggcggcggtg gtggggaagt cgcgcgtgac
     7081 gaacacgtcc ttgtgcgcga ttggcaggcc ggccagcggc gccgcctggc ccgtggccag
     7141 ggcggcatcc tgagcgcgcg cctgggccag cgtcacgtcc tcgtcgatgg ccaggtaggc
     7201 ggccaggccc tggtgcgcct tggcgcgcgc caggaaatgc tgggcggcct cgacggcgct
     7261 cacctgtttg gcgcgcagtg cggcggccag ctgggccacg cccaggtcgt gcagatcggt
     7321 gttgttgttc atggccgcgc cctcactcga tgaccctggg caccaggaac aggccgcgct
     7381 ccacggccgg ggcgctgcgc tggttggcct cgcgctgatt gggctcgctg gccacgtcgg
     7441 ccgccaggcg caggtgcacg tcctggatca ctgccgcagg gtggggcagg ggggtgacgc
     7501 cgctggtgtc caccgcctgc attgcctcga cgatgctgaa aaagtcgttg agctgcgtga
     7561 gcatgcgctc gctttcctgg ggggcgagct cgagccgggc cagatgggcg atgcggccta
     7621 tgtcctgggg tgtcagtgcc ataaggggaa aaaaggacgg cggggcgtgg gcaacggtat
     7681 gtaaacaaac atccgagtca cgctcctggg agagttattc acaggggatg cgttattatc
     7741 ccgcctttgc cgcaatgcca gcacattgcc ggccattgcg cgaaaaatac ccacaaatac
     7801 cctgtaaaac ccggcgatgg caggccgaag tgcatgccgt gcctgatgag gattcctgca
     7861 tgttcggagc tttccgtcgg tatttctcca ccgacctggc cattgacctt ggtaccgcca
     7921 acaccctgat ttttgcccgc gacaagggca tcgtactgga cgagccctcg gtcgtcgcca
     7981 tccgtcacga gggcgggccg catggcaaga aggtgatcca ggccgtgggc cgcgaggcca
     8041 aggccatgct gggcaaggtg cccggcaaca tcgaggccat ccgcccgatg aaggacggcg
     8101 tgatcgccga tttcgtcatc accgagcaga tgatcaagca gttcatcaag atggtgcatc
     8161 cgcgctcggt gctcacgccc agcccgcgca tcatcatctg cgtgccctgc ggctcgaccc
     8221 aggtcgagcg tcgtgctatt aaagatgcag ctgaagccgc tggcgccacg gccgtttacc
     8281 tgatcgagga acccatggcc gcaggcatag gcgcgggcct gccggtgtcc gaggcatcgg
     8341 gttccatggt ggtggacatc ggcggcggca ccaccgaggt cggcgtcatc agcctgggcg
     8401 gcatggtcta caagggcagc gtgcgcgtgg gcggcgacaa gttcgacgag tccatcatca
     8461 actacatccg gcgcaactac ggcatgctga tcggcgagcc cacggccgag tccatcaaga
     8521 agcatattgg ctcggccttt cccggctccg aggtcaagga gatggaggtc aagggccgca
     8581 atctgtccga gggtgtgccg cgcagcttca ccatcagctc caacgaggtg ctggaggcgc
     8641 tgaccgagcc gctcaaccag atcgtctcgg ccgtgaagac ggcgctggag cagacgccgc
     8701 ccgagctcgg cgccgacatc gccgagcgcg gcatgatgct caccggcggc ggcgcgctcc
     8761 tgcgcgacct cgaccgcctg ctggccgagg aaaccggcct gcccgtgctg gtggccgaag
     8821 agcccctgac ctgcgtggtg cgtggctgcg gcattgcgct cgagcgcatg gatcgccagg
     8881 gcagcatctt caccagcgaa taagtccacg ccgccgctgg cgcgctgttc gcatgcccct
     8941 gggaaccctg gaccgctcgg cgcccacgct gttcaagcat gggccttcgc cgctgtcgcg
     9001 cctggccctc tacagcgccc tggcgctgtt tttgatggtg gccgatgcgc gctttcgcat
     9061 caccgaaccg ctgcgcaagg tggtggccac ggcgctgtat ccgctgcaat ggctgatgct
     9121 gcaacccgtg gagctggccg acaagggcct gggctacctg cagtcgctgc aggccgcgca
     9181 gcaggcggcg gacgcggcgc aaaagcagat ggcgcagatg tcggtgcgcg ccggcgagac
     9241 cgaccagctg ctgcgcgaga acgccgagct gcgccagctg ctggcgctgc gtgcgcgcct
     9301 ggacacgccg gcccaggccg cccaggtgat ctatgacacg gccgacccgt acacccggcg
     9361 cgtcatggtc gatcgcggcc agcttgcggg cgtgcaggcc ggcgcgcccg tggtcgatgc
     9421 ctccggcgtg ctggggcagg tgacgcgcgt ctatcccatg ctcagcgagg tcaccttgct
     9481 gatagatcgc gaccaggcca ttcccgtgct caacgtgcgc accggcgcgc gcagcgtggc
     9541 ctatggcgac ccggtagccg ggctgggcgg tggcatggag ctgcgcttca cgcccggcaa
     9601 cgccgacgtg caggagggcg acctgctcac caccagcggc gtcgatggcc tgtacccgcc
     9661 gggcctgccc gtggcccgcg tggtgcgtgt cgagcgccgc gccgactcca cctttgcgcg
     9721 catctactgc gcgcccctgg cccaggtgta cggcgcgcgc catgtgatgc tgctcgagcc
     9781 gctggcgaac gacctgccgc cccgccccgc gcccgagccg gcgacgcaaa agcgcggagg
     9841 gcacaaatga tcatgcccaa gggccagcag ctgctgctgc cggtgaatcc cgtcttcatc
     9901 gtcgccagcc tggtgctggc gctggcgctg aacctgctgc cgctgggccg ggtagtctgg
     9961 acgccggact gggtcatggt gctgctggtg ttctggggca tacaccagcc gcagcgcgtc
    10021 ggtctgggtg tggcctttgc catgggcctg tgcatcgatg tcgcccagtc ggccctgctg
    10081 ggtcagcatg cgctggtgta ctgcggcgtg atgttcggcg cgcagctggc gcaccggcgt
    10141 ctgctgtggt tcgggccgct gtggcaggcg ctgcagctgc tgccgctgtt ctttgctggc
    10201 catgccgtgg agctggcgct gcgcgtggtc gccggcggcg gcaccctgcc cgggctggag
    10261 ggcctggcag cgccgctgct ggaggccgtg atctggccac tggccagctg ggttctgctg
    10321 gcgccgcagc gccgtccgcc ggatcaggac gacaatcgtc ctctgtaacc gggttggagc
    10381 acgagccatg accgagctgc gcaatgtgga ggccgacgcc agacggtttc gcctgcgcgt
    10441 gttcgtgctt ggcgtggtgg tgctgctggc cttcctgctg atcgtggcgc gcctgctggt
    10501 gctgcaggtc gagcgccacg acgacctggc cgagcaggcc gagagcaacc gcacggccat
    10561 cgtccccatc gtgcccaacc gcggcctgat catggatcgc aatggcgtgg tgctggccac
    10621 caactattcg gcctacacgc tggagatcac gccctcgcgc gcgatcgacc tggagggcac
    10681 catagaccag ctggcgcaga tcgtggacat acagcagcgc gaccggcgcc gcttcaagcg
    10741 cctgcgcgaa gagtcgcgca gcttcgactc gctgccgata cgcacgcgcc tgtccgacga
    10801 ggaggtggcg cgctttgccg cgcagcgcta ccgctttccc ggcgtggaga tcaaggcccg
    10861 gctgtttcgc acctacccca tgggcgaggt ggcgagccac gccatcggct acatcggccg
    10921 catcaaccag cgcgagaagg agcgcatcga ggactcggac gacgccgcca actaccgcgg
    10981 cacggactac atcggcaagc tgggcgtgga gcagagcttc gagccgacgc tgcacggtca
    11041 gaccggtttc gagcgcatgg agacctcggc cggcggccac gccgtgcggc gcctcaatag
    11101 ccacccggcg acgcccggca acacggtgat gctgtcgctg gacatcaagc tgcaaaagct
    11161 catcgaggag atgtttggcg agcgccgcgg cgcgctggtg gcgctggatc cgcgcaatgg
    11221 cgaggtgctg gccctggtct ccaagcccac cttcgacccg aacctgttcg tcgagggcat
    11281 agaccaggaa aactggcagg cgctgaacga gtccatcaac aagccgctgc tcaaccgcgc
    11341 gctgcgcggc acctacccgc cgggctcgac ctacaagccc ttcatggcgc tggcggcgct
    11401 ggagctgaaa aaacgctcgg ccacccaggt catcagcgac ccgggcttct acaactacgg
    11461 cgggcgcacc ttccgaagcc atgaaggtgg cctgggcggc gtggacatgc accgcgccat
    11521 ccagtattcg agcaacacct acttctactc gctgggcgtg gagatgggcg tggacgccat
    11581 ccatgacttc atgaagccgc tctccttcgg ccagatcacc ggcatcgact tagggggcga
    11641 ggtgcgcggc gtgctgccca gcatggagtg gaagcgcaac acctacaagc gccccgagat
    11701 gaagcgctgg tactcgggcg agaccgtgtc gctgggtatt ggccagggct acaacaactt
    11761 caccatgctg cagctggccg tggccgaggc gaccctggcc aacggcggca cgcgccaccg
    11821 cccgcatctc atcaaggccg tcaaggacca ggtcacgggc caggtgaccg aggtgcagca
    11881 gccgcccggc gagaacctgg gctacaaccc gaagaacgtg gccatcatca acaacgccct
    11941 gatggccgtg aaccaggccg gcacggggcg gcgcgtgttc ctgggtgcgc cctatacctc
    12001 ggccggcaag accggcacgg cccaggccgt ggcctggggc cagaacacca agtacaacgc
    12061 ccggctgctg gaggagcaca agcgcgacca ttcgctgttt gccgccttcg cgcccgtgga
    12121 agaccccaag gtggcggtgg ccgtgatcgt ggaaaacgcc ggctttggcg ccgccgcagc
    12181 cgcccccatc gtgcggcgcg tgttcgacta ctggctgctg ggccagtacc ccagcgagca
    12241 ggatctggcc gccgtgtcca agggccaggc cgctgcgccc atcggcgtgc cgcggcgcgc
    12301 agacgatgta ccgctgacgc ccgagccctg atcaggccag cgtggccgcg cgcggcggct
    12361 gcagctggct gatgatgagc gcggcgacga tcagcgccgc gcccaccagg cccaccgcgc
    12421 ccaggcgctc gccgatcaag aaccaggcgg tggcggcggc aaacaccggc tccaggccaa
    12481 agacgatggc gctgcgcatg gcgtccacgc gctgctggcc ccaggcctgc agcgtgacga
    12541 cgagcacgct ggcgatcacg cccagatagg ccagcgccgt cagcgccggg ccgggcagcg
    12601 tggcgatggc gtcccaggtg ccggcaaact cgccatggcg tgccagcatc agggcgcccg
    12661 acgccacgca catcaccagc gcctggctgg cggccatgcg cgtggcgcgc agcggagcgc
    12721 tggcggtgcg gcgcgcgcat tcttccaggc ccaggatgta gacggcgtag aacagcgtgc
    12781 tggccagcgt cagtgtgtcg cccaggttcc agggtgcgtc ctcgtggaac atcagcgtca
    12841 tgccgaccag cgccatggcg caggcggccc acagcgccag cccatagcgc cggcccagcg
    12901 ccagcatggc caagagcggc accaccagca cgttgagccc ggtgacgaag gcgttgcggt
    12961 tgctgctggt gcgcgccagc ccctcggtct gcagccagaa ggcgatgaac agcagcgcgc
    13021 ccagcagcag gccccagtgc agctcggtgc ggcgcatgcc gcgccacatg ggcagcagca
    13081 ccagcagggc gatggcaaag cgcgcccaga tgatctgcaa cgcgtccagc tgcgccgaca
    13141 gcagcttcat ggtcgggaag gtggtgcccc agacggccac cacgatgagc agggccatca
    13201 gcccctggcg ttcggagcgc attgttaggt ttaatataaa aatgggcttt agcgcttaat
    13261 cagtaagcgt cagaagctat gaaaataata gtaaatcagt ggcctacgcg gccaatgaag
    13321 gcgcggatgc gctcgttggc cgggtgggcg aagaac
//
LOCUS       DAOMTQ010000085        13070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_834_length_13070_cov_29.214599, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000085 DAOMTQ010000000
VERSION     DAOMTQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 13070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13070
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_834_length_13070_cov_29.214599"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..389
                     /gene="rpsJ"
                     /locus_tag="PK518_09430"
     CDS             78..389
                     /gene="rpsJ"
                     /locus_tag="PK518_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRM49177.1"
                     /translation="MSKQKIRIRLKAFDYKLIDQSAAEIVETAKRTGAIVKGPVPLPT
                     RMKRFDILRSPHVNKTSRDQLEIRTHQRLMDIVDPTDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            615..1289
                     /gene="rplC"
                     /locus_tag="PK518_09435"
     CDS             615..1289
                     /gene="rplC"
                     /locus_tag="PK518_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRM49178.1"
                     /translation="MSLCNSLGLLGRKVGMMRLFTDDGDAVPVTVVDVSNNRVTQVKT
                     QENDGYVALQVTFGARKASRVTKPEAGHLAKAGVEAGEIIREFPVTAEVAGKYAAGAT
                     VPVADVFAVGQKVDVQGTSIGKGYAGTIKRHNFGSQRASHGNSRSHNVPGSIGMAQDP
                     GRVFPGKKMTGHLGDVTVTTQNLDVVRIDEARQLLLIKGAVPGSKGGFVTVRPAIKAP
                     ASKGAN"
     gene            1289..1909
                     /gene="rplD"
                     /locus_tag="PK518_09440"
     CDS             1289..1909
                     /gene="rplD"
                     /locus_tag="PK518_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRM49179.1"
                     /translation="MQLELLNEQGQAASKVDVPETVFDRQYNEDLIHQIVVAYQANAR
                     QGTRAQKDREQVRHSTKKPFKQKGTGNARAGMTSSPLWRGGGRIFPNLPDENFTQKIN
                     KKMYRAGMAAILSQLAREGRLAVVDSLKLESPKTKVLADKFKAMNLQSVMVIADEVDE
                     NLYLASRNLKNVFVVEPRYADPVSLVHYKKVLVTKGAIDKLKEMFA"
     gene            1906..2220
                     /gene="rplW"
                     /locus_tag="PK518_09445"
     CDS             1906..2220
                     /gene="rplW"
                     /locus_tag="PK518_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRM49180.1"
                     /translation="MSTLKFDEGRLMQVLVAPIVSEKATMVAEKSNAVTFKVLQNATK
                     PEIKAAVELMFKVEVKGVSVVNTKGKTKRFGKTMGRRDNVRKAYVLLKEGQELNLSGE
                     AA"
     gene            2224..3048
                     /gene="rplB"
                     /locus_tag="PK518_09450"
     CDS             2224..3048
                     /gene="rplB"
                     /locus_tag="PK518_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRM49181.1"
                     /translation="MAVIKIKPTSPGRRGTVKISRDHLHKGEAFAALLEPQFQKAGRN
                     NNGHITTRHKGGGHKHHYRVVDFRRNKDAIPAKVERIEYDPNRSAHIALVCYADGERR
                     YIIAPRNLEVGSSIVSGSEAPIRVGNTLPIRNIPVGSTIHCIELKAGAGAQIARSAGT
                     SATLLAREGMYAQVRMRSGEVRKIHIECRATIGEVANEEHSLRQLGKAGVKRWMGIRP
                     TVRGVAMNPIDHPHGGGEGRTGEGRHAVDPWGNLTKGYRTRNNKRTQTMIVSRRKK"
     gene            3059..3337
                     /gene="rpsS"
                     /locus_tag="PK518_09455"
     CDS             3059..3337
                     /gene="rpsS"
                     /locus_tag="PK518_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRM49182.1"
                     /translation="MTRSLKKGPFVDHHLLAKVEKAIATKDKKPVKTWSRRSMVLPEF
                     IGLTIAVHNGKQHVPVYVTDQMVGHKLGEFALTRTFKGHPADKKVQKK"
     gene            3347..3679
                     /gene="rplV"
                     /locus_tag="PK518_09460"
     CDS             3347..3679
                     /gene="rplV"
                     /locus_tag="PK518_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRM49183.1"
                     /translation="MSETRAVLRGVRLSVDKGRLVADLIRGKKVDQALNILAFTQKKA
                     AVIVKKVLESAIANAEHNDGADIDDLKVKTIFVEQGTTLKRFTARAKGRGNRISKPTC
                     HIYVTVGN"
     gene            3696..4565
                     /gene="rpsC"
                     /locus_tag="PK518_09465"
     CDS             3696..4565
                     /gene="rpsC"
                     /locus_tag="PK518_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRM49184.1"
                     /translation="MGQKIHPTGFRLSVSRNWASRWYASNRDFAGMLAEDIKVREFLK
                     AKLKNAAVSRILIERPAKNARITIFSARPGVVIGKKGEDIENLKKELATRLGVPVAVN
                     IEEVRKPEIDAKLIADSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNGI
                     EIARTEWYREGRVPLHTLRADIDYGTSEAKTTYGVIGVKVWVYKGDTLGRGDLPAAET
                     PRPEEERRPRGPRRDGRGDGRRDGAGRGGRRPMGANAAPADGSDKPAGAGADSVKRVR
                     KDAPASAADGKGE"
     gene            4568..4984
                     /gene="rplP"
                     /locus_tag="PK518_09470"
     CDS             4568..4984
                     /gene="rplP"
                     /locus_tag="PK518_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRM49185.1"
                     /translation="MLQPARRKFRKEQKGRNTGIATRGNSVAFGDFGLKCTDRGRLTA
                     RQIEAARRAISRHVKRGGRIWIRVFPDKPISTKPAEVRMGNGKGNPEYYVAEIQPGKV
                     VFEIVGVPEELAREAFRLAAAKLPLRTTFVARQIGA"
     gene            4996..5193
                     /gene="rpmC"
                     /locus_tag="PK518_09475"
     CDS             4996..5193
                     /gene="rpmC"
                     /locus_tag="PK518_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRM49186.1"
                     /translation="MTKAAELRQKDVAGLEAEIKSLQKAHFGLRMQKATQQLGNTNTL
                     RTTRRDIARAKTILAEKQAAK"
     gene            5207..5476
                     /gene="rpsQ"
                     /locus_tag="PK518_09480"
     CDS             5207..5476
                     /gene="rpsQ"
                     /locus_tag="PK518_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRM49187.1"
                     /translation="MTEAKPSLKRTLVGKVVSDKRTKTVTVLVERRVKHPIYDKIMIK
                     SSKYHAHDENGEYKMGDTIEITESRPLSKTKNWVATRLVQKAALV"
     gene            5556..6062
                     /locus_tag="PK518_09485"
     CDS             5556..6062
                     /locus_tag="PK518_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRM49188.1"
                     /translation="MIKVGDQLPATTLMEYIEVEGNGCSMGPNAVDVHKAAAGKTIAL
                     FAVPGAFTPTCSNQHVPGYVAQADALKAAGVDEIWCLSVNDAFVMGAWARDQKTANKV
                     RMLADGDAAFAKATGLTLDLNGKGLGLRSNRYSMLVKDGKVASLNVEAPGKFEVSDAA
                     TMLGQLKA"
     gene            complement(6180..6623)
                     /locus_tag="PK518_09490"
     CDS             complement(6180..6623)
                     /locus_tag="PK518_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM49189.1"
                     /translation="MDAVRAIFQEYADSLDVDLEFQNFADELAHLPGEYSAPRGLLLL
                     AEVDGSIAGCCGLRPLDSVDYANAGEMKRLYVRKAFRGFGLGRQLAEAILDAAHQAGY
                     ACVLLDTLDGMEAARALYAELGFTEIPPYYHNPVSGSHYLKADIG"
     gene            complement(6725..7390)
                     /locus_tag="PK518_09495"
     CDS             complement(6725..7390)
                     /locus_tag="PK518_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRM49190.1"
                     /translation="MQHKVRVWDLPTRLFHWFLAATVVALVITAKVGGDAMQWHLRLG
                     HVMLALLLFRLVWGVVGGHWSRFASFVHSPTRLWRHLRGQASEETAGHSPLGALSVFA
                     LLAVLLAQVGSGLLSDDEISFSGPLVRFVSGEWVGLATWFHSDLGQYLLIGLVALHLL
                     AIGYYVLVRRRTLVRPMLHGDKLLTRQAPASRDDVAARTGAAVVAVLSAALAWWISGL
                     GAG"
     gene            7563..8024
                     /locus_tag="PK518_09500"
     CDS             7563..8024
                     /locus_tag="PK518_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRM49191.1"
                     /translation="MTFKTLAISAAVAVTTLLALPVHAQFAKSEEAVKYRQGSLFVIG
                     QHFGRIGAMVNGKLPFDAKAAQENADIVAAMAALPWTGFGPGTDKGAPHKAKPNVWTE
                     KAKFNEHAQTFQAEAAKLASAAKTGNLEQIKTAFGPAAQSCKSCHDAFRNR"
     gene            complement(8112..8615)
                     /locus_tag="PK518_09505"
     CDS             complement(8112..8615)
                     /locus_tag="PK518_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRM49192.1"
                     /translation="MQIKHWLAAGVVAAGVAIGAVVVLGSGTSVAPQSTFVLLDGSQQ
                     TTADLRGKVTLVNFWATSCTTCVAEMPDIVATYDKYKGQGFETLAVAMSYDPPSYVVN
                     FTETRKLPFKVAIDNTGAVAKAWGDVKLTPTTYIVNKRGEIVKSYVGAPDFTELHRLI
                     EKLLAEA"
     gene            8837..9284
                     /locus_tag="PK518_09510"
                     /pseudo
     CDS             8837..9284
                     /locus_tag="PK518_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592917.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            9256..9525
                     /locus_tag="PK518_09515"
     CDS             9256..9525
                     /locus_tag="PK518_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM49193.1"
                     /translation="MIKNSITISNKLGLHARASAKLTKLAGSYPCEVWMSKGERRINA
                     KSIMGVMMLAAGLGTQVEVETDGAQEQEAMNALLALIDDKFGEGE"
     gene            9544..11313
                     /gene="ptsP"
                     /locus_tag="PK518_09520"
     CDS             9544..11313
                     /gene="ptsP"
                     /locus_tag="PK518_09520"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRM49194.1"
                     /translation="MSFSIHGLSVARGIAIGRAVLATSSRMEVAHYFIEPTQVQAEIA
                     RVRNGRNAVVDELQRLRADLPPDAPSELAALLDVHLLLLQDEMLVSGVKHWITDRLYN
                     AEWALTTQLEIISRQFDEMEDDYLRERKADLEQVVERILRRMKGVASPVAPPPRSPRR
                     QAEAGAEAEFLQGGAADVPLVLVAHDLSPADMLQFKQSVFTGFITDVGGKTSHTAIVA
                     RSMDIPAVVGARGASQLVRQDDWVIIDGDAGAVIVDPSPIILAEYGFKQRQIALERER
                     LTRLRHTPAVTLDAQAIELLANIEQPGDVASALRAGAEGVGLFRSEFLFMGRAGRMPD
                     EEEQYQAYREAVEGMQGLPVTIRTIDVGADKPLDKSHKDSSLNPALGLRAIRWSLADP
                     AMFRTQLRAVLRAAVHGPVKLLFPMLAHVGEIRQTLAQVDLARAELDARGMAYGPVQL
                     GAMIEVPAAALMVQQFLHYFDYLSIGTNDLIQYTLAIDRADEAVADLYDPLHPAVLRL
                     VADVIAAGASQGKSVSVCGEMAGDVGMTRLLLGLGLRSFSMQPAQILAVKQEVLRADA
                     RKLADWAQQVLAADDPASYMNAA"
     gene            complement(11310..12017)
                     /gene="lipB"
                     /locus_tag="PK518_09525"
     CDS             complement(11310..12017)
                     /gene="lipB"
                     /locus_tag="PK518_09525"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRM49195.1"
                     /translation="MNDQAVQVRLLGRVDYSETVAAMQSFTVQRTADTPDELWICEHA
                     AVYTQGFAAKNNHILAPGDIPVVATNRGGQVTHHGPGQVVAYPLLDLQRAGYYVKEYV
                     YRLEESLIRTLAHYDVTGHRVDGAPGIYVRLDDPGGHAMLAQRPQRRTPGSPRPQPDF
                     TGLGKIAAIGIKVSHHCTYHGVALNVAMDLEPFSRINPCGYAGLQTVDLASIGVKTDW
                     ADAAAVLGRQLALRLAP"
     gene            complement(12014..12310)
                     /locus_tag="PK518_09530"
     CDS             complement(12014..12310)
                     /locus_tag="PK518_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HRM49196.1"
                     /translation="MTTPADIPASDPRKDSLIEYPSRFPIKVMGAKVDGFVHAIVELA
                     RRHDPSFDASTIELRDSRAGNYLGVTITITATSREQLDDLYRALSSHPMVKVVL"
     gene            12522..13010
                     /locus_tag="PK518_09535"
     CDS             12522..13010
                     /locus_tag="PK518_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRM49197.1"
                     /translation="MAQASTREKVTASEAESEVEDSDFKPLTAQQAQEWRDRQPPLSV
                     WRIVVMQALVGVVVALLAWWLTGRLQVAWSAGYGALSVVLPAALFARGMARKRTSAGA
                     AMAGLMGWELVKIALTVAMLAAAPRLVPGLSWLALLAGMVITMKTYWIALLARPGVRR
                     TD"
BASE COUNT         2581 a         4077 c         4187 g         2225 t
ORIGIN      
        1 gcgaaggcgg ccgcaccgtg ggtgccggcg tcgttgccaa gatcattgcg taatcctgca
       61 atcacaagga atacccaatg tccaagcaaa agatccgtat ccgcctgaag gcgtttgatt
      121 acaagctgat cgaccagtcg gctgccgaga tcgtggaaac cgccaagcgc accggcgcca
      181 tcgtcaaggg ccccgtgccc ctgcctacgc gcatgaagcg tttcgacatc ctgcgttcgc
      241 cgcacgtcaa caagaccagc cgtgaccagc tggagatccg cacgcaccag cgcctgatgg
      301 acatcgtcga tcccacggac aagaccgtgg acgcgctgat gaagctcgac ctgccggccg
      361 gcgtggacgt agagatcaag ctgcagtaac ccctgtcgcc gatcgggcgt ggccgtgagg
      421 ccgcgtcaca actaacgcgg acttgcttga aaaggcagtc cgcgttatac tttgcggctt
      481 cgcgtttttg cgcctcggtt tctggggtgc ggccgcggag ttttatcaac cttctttcac
      541 gcaccctgaa agccagtcgg tgcaaacgcc tgggccaatt gcagtcgagg cggtggaagt
      601 tttggagaaa accaatgagt ctgtgcaact ccctcgggtt gctgggccgc aaggtgggca
      661 tgatgcgtct gttcaccgat gacggggacg cagtgcccgt cacggtggtg gatgtgtcca
      721 acaaccgcgt gacccaggtg aaaacccaag agaacgatgg ctacgtggcc ctgcaggtca
      781 cgttcggcgc acgcaaagct tcgcgtgtga ccaagccaga agccggccac ctcgccaagg
      841 cgggtgtcga agccggtgaa atcatccgcg aattccctgt gaccgctgaa gtcgctggca
      901 agtacgccgc aggcgctact gtgcccgtcg ccgacgtgtt cgccgtgggt cagaaggtgg
      961 acgtgcaagg tacttccatc ggcaagggct acgccggcac catcaagcgc cacaacttcg
     1021 gttcgcagcg cgcctcgcac ggtaacagcc gctcgcacaa tgttcctggc tccatcggca
     1081 tggcgcagga tccgggccgc gtgttccctg gcaagaagat gaccggtcac ctcggtgacg
     1141 tgacggtcac gacgcaaaac ctcgacgtgg ttcgcatcga cgaagcacgc caactgctct
     1201 tgatcaaggg cgctgttccg ggctccaagg gtgggttcgt tacggtgcgt ccggccatca
     1261 aggcgccagc ttccaaagga gcgaactaat gcagctcgaa ctcctgaatg aacaaggcca
     1321 ggcggcatcc aaggtggatg tgcccgagac cgtgtttgac cgccagtaca acgaagacct
     1381 gatccaccag atcgtcgtcg cctaccaggc caatgcccgc caaggcacgc gtgcccagaa
     1441 ggaccgcgag caggtgcgtc actcgaccaa gaagcccttc aagcaaaagg gcaccggcaa
     1501 cgcacgtgct ggtatgacct cctcgccact gtggcgtggg ggcggtcgca tcttcccgaa
     1561 cctgcctgat gaaaacttca cgcagaagat caacaagaag atgtaccgcg ccggcatggc
     1621 cgccatcctg tcgcagctgg cccgcgaagg ccgcctggcc gtggtggact cgctgaagct
     1681 ggaaagcccc aagaccaagg tgctggccga caagttcaag gccatgaacc tgcagtcggt
     1741 gatggtgatc gccgatgagg tcgatgaaaa cctgtacctc gcctcgcgca atctgaagaa
     1801 cgtgttcgtc gtcgagccgc gttatgcaga ccccgtgtcg ctggtgcact acaagaaagt
     1861 gctcgtcacc aagggcgcga tcgacaaact caaggagatg ttcgcatgag cacgctgaag
     1921 tttgacgaag gtcgtctgat gcaggtgttg gttgctccca tcgtgtccga aaaggccacc
     1981 atggttgccg agaagtccaa cgccgtgacg ttcaaggtac tgcagaacgc aaccaagccc
     2041 gagatcaagg ccgccgtgga actgatgttc aaggtggaag tgaagggcgt ttctgtggtg
     2101 aacaccaaag gcaagaccaa gcgctttggc aagaccatgg gccgtcgcga caacgtgcgc
     2161 aaggcctatg tgctgctcaa ggaaggtcaa gagctgaacc tgtccgggga ggctgcgtaa
     2221 accatggccg ttatcaagat caaaccgact tccccgggcc gtcgcggcac ggtgaagatc
     2281 tcgcgtgacc acctgcacaa gggtgaggcg ttcgcagcct tgctggaacc ccagttccag
     2341 aaggccggcc gcaacaacaa cggtcacatc accacccgtc acaagggcgg tggccacaag
     2401 caccactacc gcgtggtgga cttccgtcgc aacaaggacg ccatccccgc caaggtggag
     2461 cgcatcgagt acgaccccaa ccgctcggcc catatcgccc tggtgtgcta tgccgacggc
     2521 gagcgtcgct acatcatcgc cccgcgcaac ctggaagtgg gcagctccat cgtgagcggc
     2581 tccgaggccc cgatccgcgt cggcaatacg ctgccgatcc gcaacatccc ggtgggctcg
     2641 accatccact gcatcgagct gaaagccggt gccggtgccc agatcgcccg ctcggccggt
     2701 acctcggcca cgctgctggc gcgcgaaggc atgtacgccc aggtgcgcat gcgctccggc
     2761 gaagtgcgca agatccacat cgagtgccgc gccaccatcg gtgaagtggc caacgaagag
     2821 cacagcctgc gccagttggg caaggccggt gtcaagcgct ggatgggcat ccgcccgacg
     2881 gtgcgcggtg tggccatgaa cccgatcgac cacccgcacg gtggtggtga gggtcgtacc
     2941 ggcgaaggcc gtcacgcagt tgacccatgg ggcaacctga cgaagggcta tcgcacccgc
     3001 aacaacaagc gcacgcagac catgatcgtc tcgcgtcgca agaagtaaga ggtagcaaat
     3061 gactcgttct ctcaaaaagg gtccgtttgt tgaccatcac ttgctggcca aggtcgagaa
     3121 ggccatcgcc accaaggaca agaagccagt caagacctgg tcgcgtcgct ccatggttct
     3181 gcccgagttc atcggtctga ccattgccgt gcataacggc aagcagcatg tgccggttta
     3241 tgtcaccgac cagatggtgg gccacaagct gggcgaattc gccctgacgc gcaccttcaa
     3301 gggtcacccc gcggacaaaa aagtccagaa gaagtaagga acgaccatgt ctgaaacacg
     3361 tgcagtcctc cggggcgtcc gtttgtcggt ggacaagggt cgcctggtcg ctgatctgat
     3421 ccgcggcaag aaggtggatc aggctctgaa catcctggct ttcacgcaga aaaaagctgc
     3481 cgtgatcgtc aagaaggtgc tggagtccgc catcgccaac gccgagcaca acgacggcgc
     3541 cgacatcgac gatctgaagg tcaagaccat cttcgtcgag cagggcacca cgctcaagcg
     3601 cttcacggcg cgcgccaagg gccgcggcaa ccgcatcagc aagccgacgt gccacatcta
     3661 cgtgaccgtg ggcaactaaa ggccagagga agaatatggg acagaaaatc catcctaccg
     3721 gcttccgcct gtcggtcagc cgtaactggg ccagccgctg gtacgcgagc aaccgtgact
     3781 tcgccggcat gctggccgaa gacatcaagg tgcgcgagtt cctgaaggcc aagctgaaga
     3841 acgccgcggt ctcgcgcatc ctgatcgagc gccccgccaa gaacgcccgc atcaccattt
     3901 tctcggcacg tccgggcgtg gtcatcggca agaagggcga ggacatcgag aacctgaaga
     3961 aggaactcgc tacccgcctg ggcgtgcctg ttgctgtcaa catcgaggaa gtgcgcaagc
     4021 ccgaaatcga tgccaagctg atcgccgact cgatcaccca gcagctggaa aagcgcatca
     4081 tgttccgccg cgccatgaag cgcgccatgc agaacgccat gcgtctgggt gcccagggca
     4141 tcaagatcat gtccgctggc cgtctgaacg gcatcgaaat cgcccgtacc gagtggtacc
     4201 gcgaaggccg tgtaccgctg cacaccctgc gcgccgacat cgactacggc acctccgaag
     4261 ccaagaccac ctatggtgtg atcggcgtga aggtctgggt ctacaagggc gacacactgg
     4321 gtcgtggcga cctgcctgct gccgagacgc cgcgccccga agaggagcgc cgcccgcgtg
     4381 gcccgcgccg cgacggccgt ggtgatggcc gccgtgacgg tgccggccgt ggtggccgcc
     4441 gccccatggg cgccaacgca gcacctgccg acggtagcga caagcccgct ggcgctggtg
     4501 cagattccgt taagcgcgtt cgtaaagacg cgcccgcttc agctgcggac ggtaaaggag
     4561 aataaacatg ctgcaacctg ctcgccgcaa gttccgtaag gagcaaaaag gccgcaacac
     4621 cggtatcgcc acgcgtggca attcggtggc attcggtgac ttcggtctga agtgcacgga
     4681 tcgtggccgt ctgacggccc gccagatcga ggccgcacgt cgtgcgatct cgcgtcacgt
     4741 caagcgtggc ggtcgtatct ggatccgcgt gttccccgac aagccgattt ccaccaagcc
     4801 cgcagaagtg cgtatgggta acggcaaggg caaccccgag tactacgttg ccgagatcca
     4861 gcccggcaag gtggtgttcg aaatcgttgg cgttcccgaa gaactggccc gcgaagcgtt
     4921 ccgcctggct gccgccaagc tgccgctacg caccaccttc gttgcccgtc agattggcgc
     4981 ctaaggagaa tcaacatgac caaagctgct gaactccgcc aaaaggacgt ggccggcctc
     5041 gaagccgaaa tcaagtcgct gcaaaaggcc catttcggcc tgcgcatgca gaaggccacg
     5101 caacagctgg gcaacaccaa cacgctgcgt accacgcgcc gtgacattgc ccgcgccaag
     5161 accattcttg ctgaaaagca agccgccaag taatccagga gcgaacatga cggaagccaa
     5221 accctccctc aagcgcacct tggttggcaa ggtggtcagc gacaagcgta ccaagaccgt
     5281 gaccgtgctc gtcgagcgcc gcgtcaagca ccccatctac gacaagatca tgatcaagtc
     5341 gagcaagtac cacgcccacg acgagaacgg cgagtacaag atgggcgaca ccatcgagat
     5401 cacggaaagc cgtccgcttt ccaagaccaa gaactgggtg gcgacccgcc tggtgcaaaa
     5461 ggccgctctg gtctaagcct tatcggctcc cgggcagcaa tgcccatgga aacgacccac
     5521 aatgtgggtc gtttttttat tccaaggagt cgagcatgat caaagtcggt gaccaacttc
     5581 ccgccaccac cctgatggaa tacatcgagg tcgagggcaa tggctgcagc atgggcccca
     5641 atgccgtgga tgtgcacaag gctgccgcag gcaagaccat tgcgctgttc gccgtgccgg
     5701 gtgccttcac ccccacctgt tccaaccagc atgtgccggg ctatgtggcg caggccgatg
     5761 ccctgaaggc tgccggcgtg gatgagatct ggtgcctgtc ggtgaacgat gccttcgtga
     5821 tgggcgcctg ggcacgggat cagaagacgg ccaacaaggt gcgcatgctg gccgacggtg
     5881 acgctgcctt tgccaaggcc accggcctga cgctggactt gaacggcaag ggcctgggcc
     5941 tgcgcagcaa ccgctattcc atgctggtca aggatggcaa ggtggccagc ctgaacgtgg
     6001 aagcgcccgg caagttcgag gtcagcgacg ccgcaaccat gctcggtcag ctcaaggcct
     6061 gataaggttc caccaaggtc aatgccgcac cagcccgtgg gctggtgcgg catttactat
     6121 tatatgtata gcttctcgcg catgattgaa gcgcgccaga ggccaaaatt attccaattt
     6181 caaccgatat ccgccttcag gtaatgcgac ccgctcaccg ggttgtggta atagggcggt
     6241 atctcggtaa aacccagttc ggcatacagc gcgcgcgcgg cctccatgcc gtccagcgta
     6301 tcgagcagca cgcaggcgta gcctgcctgg tgtgcggcat ccagaatggc ctcggccagc
     6361 tggcgtccca ggccgaagcc gcgaaacgcc ttgcgcacgt acagacgctt catctcaccg
     6421 gcattggcat agtccacgct gtccagcggc ctcaggccgc agcagccggc gatgctgcca
     6481 tccacctcgg ccagcagcag caggccgcgc ggggcgctgt actcgccggg caaatgggcc
     6541 agctcatcgg cgaagttctg aaattccaga tccacgtcca ggctgtcggc atattcttgg
     6601 aagatggcgc gcacggcatc catctcgtgg gcttggctgg gtatgaacag gcggatggag
     6661 ggtgcgggat ccactgtgcg aaaacggggg cgggtagcgg aagacgttgc agtttagcgg
     6721 caagtcagcc agcgccaagc ccggatatcc accacgccag cgcggcgctc agcaccgcca
     6781 ccacggctgc accggtgcgc gccgccacat cgtcgcgcga tgcgggtgcc tggcgggtca
     6841 gcagcttgtc gccatgcagc atggggcgca ccagagtccg gcgccgcacc agcacgtagt
     6901 agccaatggc cagcaggtgc agcgcgacca ggccgatcag caggtattgc cccaggtcgg
     6961 aatgaaacca ggtggccagg ccgacccatt cgcccgaaac gaagcgcacc agcggcccgg
     7021 aaaatgagat ttcgtcatcg ctcagcaggc cactgccgac ctgggccagc aaaacggcga
     7081 gcagggcgaa gaccgagagt gcgcccagcg gtgaatgtcc tgccgtctcc tcgctggcct
     7141 gaccgcgcag atggcgccac aaccgcgtgg gtgaatgcac aaagctggca aaacgtgacc
     7201 aatgcccgcc caccacgccc cagaccaggc gaaacaacag cagggccagc atcacatgcc
     7261 ccagccgcag gtgccattgc atggcgtcgc cgccgacctt ggcggtgatc accagtgcca
     7321 ccaccgtggc tgccagaaac caatgaaaca aacgggtggg aagatcccag acgcgaacct
     7381 tgtgctgcat gaattgcgaa acctgaactg gaaaccggcg aattgtgccc agcggcagat
     7441 ggcgccaaga aaatccgagg aaaccctggg gtcagataca tgcgctttac gttgcacggt
     7501 gatactgcgc gcggcctggc gcaggcgccg gccatgcaga tgccaacaac aaccggattc
     7561 ccatgacctt caagaccctt gccatttccg ccgcagtcgc tgtaaccacc ttgctcgcat
     7621 tgcctgtgca tgcccagttc gccaagtccg aagaggccgt caagtaccgc cagggcagcc
     7681 tgttcgtgat cggtcagcac tttggccgca tcggtgccat ggtcaacggc aagctgccgt
     7741 ttgatgccaa ggcggcgcag gaaaacgctg acatcgtggc cgccatggcg gcgctgccct
     7801 ggaccggctt tggcccggga accgacaagg gcgcgccgca caaggccaaa cccaacgtgt
     7861 ggaccgagaa ggccaagttc aatgagcatg cccagacctt ccaggccgag gcggccaagc
     7921 tggccagtgc ggcgaagacc ggcaacctgg agcagatcaa aaccgccttt ggcccggcag
     7981 cgcagtcctg caagtcttgc cacgacgcct ttcgcaatcg ttaagctatc aaaaatatag
     8041 cttcaggcgt actgtctata agcgcaaaag gccggtttcg ttaatgaaac cggccttttg
     8101 cgttgcatgc ctcaggcctc ggccagcagc ttttcaatca agcggtgcag ttcggtgaag
     8161 tcgggggcgc cgacatagct cttcacgatc tccccgcgct tgttgacgat gtaggtggtg
     8221 ggagtgagct tgacgtcgcc ccaggccttg gcgacggcac cggtgttgtc gatggccacc
     8281 ttgaagggca gcttgcgcgt ttcggtgaag ttcaccacat aactcggcgg atcgtagctc
     8341 atggccacgg ccagggtctc gaaaccctgg cccttgtact tgtcgtaggt ggcgacgata
     8401 tcgggcatct cggcgacaca ggtggtgcag ctggtggccc agaagttcac cagggtgacc
     8461 ttgccccgga ggtcggccgt ggtctgctgt gacccatcga gcagcacaaa ggtcgattgc
     8521 ggcgccaccg atgtcccgga gcccagcacc accacggccc cgatggcgac gcctgcggcc
     8581 accacgcctg ccgccagcca atgcttgatc tgcataccct gttcctcatg ccggcccggt
     8641 gcgggcccca aatgcgttgc gattgtgccg cagcgctgga ttcgccgccg ctggcatggg
     8701 attgcgccag gagcgcgaag tttcatccgg ccttggtctg gcgcccggca gtttcatacc
     8761 ccgtggctgg cccgccgcgc cgcagaccgc tggcatggca tgggcacggt gataatgcat
     8821 ggcaaagcct tacgccatgc atacccgtct tttcatcatc gcccacgccc ccctggccag
     8881 cgcgctgcgc gaatgcgccg tgcatgtgtt tcctgattgc gcggctgacc tgctggtgct
     8941 ggacgtgcca ccccacgagc cgccggagca gaccctggcg gccgcgcggg ccttgctgga
     9001 gccactgggt gcggcttcgg tgctggtgct gaccgatgtg gttggcgcca cgccctgcaa
     9061 tgttgcgcag cgcctggtcg atgatgtgca ttcacatctg ctcgccggcg tgaacctgcc
     9121 catgctgctg cgtgcctggt gctaccgcgc ggagccgctg gacgccttgg ccaagcgcgc
     9181 cctggccggc gccacccagg gtgtgatgcg ggtcgatggc gccagcgccg cagctccaaa
     9241 accgatgtgc catccatgat caagaacagc atcaccatca gcaacaagtt gggtctgcac
     9301 gcccgtgcct cggccaagct caccaagctt gcgggcagct atccctgcga ggtctggatg
     9361 agcaagggcg agcgccgcat caacgccaag agcatcatgg gcgtgatgat gctggccgca
     9421 ggcctgggta cgcaggtcga ggtggagacc gatggcgcgc aggagcagga ggccatgaac
     9481 gcgctgctgg ccctgataga cgacaagttt ggcgaaggcg aataagtcac gagcgaggcg
     9541 cacatgagct tctccatcca cggcctgtcc gtcgcgcgcg gcattgccat cgggcgtgcg
     9601 gtactggcca cgtccagtcg catggaggtg gcgcattact tcatcgaacc aacgcaggtg
     9661 caggccgaga tagcgcgcgt gcgtaatggc cgcaatgccg tggtcgatga gttgcagcgt
     9721 ctgcgtgccg acctgccgcc ggacgcgccg tccgaactcg cggcgctgct ggatgtgcac
     9781 ctgctgctgc tgcaggacga gatgctggtc agcggcgtga agcactggat caccgaccgc
     9841 ctgtacaacg ccgaatgggc gctgaccacg cagctggaga tcatcagccg ccagttcgac
     9901 gagatggagg acgactacct gcgcgagcgc aaggccgatt tggagcaggt ggtcgagcgc
     9961 atcctgcgcc gcatgaaagg cgtggccagc cccgtggcgc cgccgccgcg cagcccgcgc
    10021 cgccaggccg aggccggcgc cgaggccgag ttcctgcagg gcggcgccgc cgacgtgcca
    10081 ctggtgctgg tggcacatga tctgtcgccg gccgacatgc tgcaattcaa gcaaagcgtg
    10141 ttcacgggct tcattaccga tgtgggcggg aagaccagtc acacggccat cgtggcgcgc
    10201 agcatggaca ttcctgccgt cgttggcgca cgcggcgcca gccagctggt gcgccaggac
    10261 gactgggtga tcatcgacgg ggacgcgggg gcggtgatcg tcgacccgtc gcccatcatc
    10321 ctggccgagt atggtttcaa gcagcgccag atcgcgctgg agcgcgagcg cctgacgcgc
    10381 ctgcggcaca cgccggcggt gacgctggat gcccaggcga tcgaactgct ggcgaacatc
    10441 gagcaacccg gcgatgtcgc atctgcgctg cgcgcggggg cggaaggtgt cggactgttt
    10501 cgcagcgagt ttctgttcat ggggcgcgcc ggacgcatgc ccgacgagga ggagcagtac
    10561 caggcctacc gtgaggcggt ggagggcatg cagggcctgc cggtcaccat acgcaccatc
    10621 gatgtgggcg cggacaagcc gctggacaag tcgcacaagg acagttcgct caacccggcg
    10681 ctgggcttgc gcgccattcg ctggagcctg gctgacccgg ccatgttccg cacccagcta
    10741 cgtgccgtgc tgcgcgccgc agtgcatggg ccggtcaagc tgctgtttcc gatgctggcg
    10801 catgtgggcg agatccgcca gacgctggcc caggttgacc tggcgcgtgc cgagctggac
    10861 gcgcgcggca tggcctacgg cccggtgcag ctgggcgcga tgatcgaggt gcctgccgcg
    10921 gcgctgatgg tgcagcagtt cctgcactat ttcgactacc tctcgatagg cacgaacgac
    10981 ctcatccagt acacgctggc cattgaccgc gccgacgagg ccgtggccga tctgtacgac
    11041 ccgctgcacc cggccgtgct gcgcctcgtg gccgatgtca ttgccgcggg cgcgagccag
    11101 ggcaagagcg tgagcgtctg cggcgagatg gccggcgacg tgggcatgac ccgcttgctg
    11161 ctcggcctgg gtctgcgcag cttttccatg cagccggcgc agatcctggc cgtcaagcag
    11221 gaggtgctgc gcgccgatgc gcgcaagctt gccgattggg cgcagcaggt gctggcggcg
    11281 gatgacccgg caagctatat gaatgccgct tagggcgcga ggcgcagtgc aagctgtcgg
    11341 cctagcacgg ctgccgcgtc agcccagtcg gtcttgacgc cgatgctcgc caggtccacc
    11401 gtttgcaggc cggcataccc gcagggattg atgcgggaga acggctccag atccatggcc
    11461 acattcaggg ccacgccatg gtacgtgcag tggtgcgaca ccttgatgcc aatcgcggcg
    11521 atcttgccca ggccggtgaa atcgggttgc gggcgggggc tgccaggcgt gcgcctttgc
    11581 gggcgctgcg ccaacatggc gtggccaccg ggatcatcca ggcgcacgta gatgccaggc
    11641 gcgccgtcca cacggtggcc ggtcacgtca tagtgcgcca gggtgcggat caaggactcc
    11701 tccagccggt acacatattc ctttacgtag tagccggcgc gctgcagatc cagcagcgga
    11761 taggccacca cctgtcccgg gccatggtgc gtgacctgcc cgccgcggtt ggtggccacg
    11821 acagggatgt cgcctggggc aagaatatga ttgtttttag cggcaaagcc ttgcgtataa
    11881 accgcggcat gctcacaaat ccatagttca tccggggtgt cggcggtgcg ctgcacggtg
    11941 aagctctgca tggcggccac ggtttcgctg tagtccaccc gacccagaag ccgaacctgc
    12001 acggcctggt cgttcataac acgaccttca ccatggggtg cgaggacagc gcgcggtaca
    12061 ggtcatcgag ctgctcgcgg ctggtggccg tgatcgtgat ggtcacgccc aggtagttgc
    12121 cggcacggct gtcgcgcaac tctatggtgg aggcatcgaa gctcgggtca tggcgccgtg
    12181 caagctcgac gatggcgtgc acgaaaccat ctaccttcgc gcccatcact ttgatgggaa
    12241 agcgcgaggg gtactcgatg agggagtcct tgcgcgggtc ggatgcgggg atgtcggctg
    12301 gggtggtcat ggtggagctt tcgtggttat gcgctggcag gtcaggcggg tgcgtacgtc
    12361 gtcggcttcg acgccgaggc actgggtgta cggcggcctt cgttggcgtg caaccacacg
    12421 gcagcccctt ggcgtctcgc tttgtatgtg tgatggtcac gcaattgtgg cgcggccggg
    12481 gtttttacct ataatgaagg gctttgtgag aatctcgatc cgtggcgcag gcatctaccc
    12541 gcgagaaagt taccgcctct gaggctgaat ctgaggttga agattcggac ttcaagcccc
    12601 tgactgcgca gcaggcccag gaatggcgtg atcgccagcc gccgttgtcg gtgtggcgca
    12661 tcgttgtcat gcaggctctg gtgggagtgg tggtggcgct gctggcgtgg tggctgacgg
    12721 ggcggttgca agtggcctgg tctgctggct atggcgcctt gtcggtggtg ttgccggcgg
    12781 cgctgtttgc gcgcggcatg gcgcgcaagc gcacatccgc cggggcggcc atggccgggc
    12841 tcatgggttg ggagttggtg aagattgctt tgaccgttgc catgttggcg gcggcgccca
    12901 ggctggtgcc ggggctcagt tggctggcgt tgctggccgg catggtgata acgatgaaaa
    12961 cgtactggat cgcgctgctg gcgcggcccg gtgtccgacg aaccgattga tatttgagag
    13021 aagagttgtc cgatggccgc agaagcgaat gcaccaaccg ccagtgaata
//
LOCUS       DAOMTQ010000086        12808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_870_length_12808_cov_27.263624, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000086 DAOMTQ010000000
VERSION     DAOMTQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_870_length_12808_cov_27.263624"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..1023)
                     /locus_tag="PK518_09540"
     CDS             complement(331..1023)
                     /locus_tag="PK518_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM49198.1"
                     /translation="MARLPRLTVPGYPHHVIQRGNNRQMIFRDAADHEFMLGLLTEQA
                     KKLQVEIHAYVLMGNHFHLLATPQTQDGLPLMMQSVGRSYVRYFNRRHQRSGTLWEGR
                     YRSTIVQSERYLLACMVYIDLNPVRAGMVAQPGDYPWSSYAHSMGRRNDPLVTPHALF
                     WSLGNTPFAREAAYGELVRAGIVGADQAQIGQSVHAGWALGEADFIAALQQRTQRRVA
                     KGKAGRPAIQTG"
     gene            complement(1081..1977)
                     /locus_tag="PK518_09545"
     CDS             complement(1081..1977)
                     /locus_tag="PK518_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM49199.1"
                     /translation="MSRAQSKADAAQAAQAAVLAELRPGQSVELLKELHILTREGRLN
                     QDSRRKLKQVYHLYQFIEPLLAELSADGHAPLLADHGAGKSYLGFILYDLFFKSLGRG
                     EIYGIEWRAELVERSRALAQRLGFERMQFLDMAVADAAALSGLPERFDIVTALHACDT
                     ATDDAIAFGLQKQARAMVLVPCCQAEVAASLRQTKALSLARTPLAELWRHPIHTRELG
                     SQITNVLRCLHLEASGYQVTVTELVGWEHSLKNELILARHTGHKKHSARERLAAILEE
                     FGLTQALGPRFGVQTLLGSDSN"
     gene            complement(1974..2603)
                     /locus_tag="PK518_09550"
     CDS             complement(1974..2603)
                     /locus_tag="PK518_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HRM49200.1"
                     /translation="MTTAPDQPNPADYDDAVVVQDTVRWLERAVIGLNLCPFAKGVHT
                     KDQIHYAVSHATDARELLQDLQRELEALAEAPAEKRDTTLLMAPLAMPDFLDFNDFLE
                     LADELVEAMDLAGILQVASFHPRFQFEGTLADDVSNCTNRAPYPTLHLLREESLDRAV
                     EVFPEAEEIFERNIEVLERLGMQGWVDLDVGARCPMGHGGKKDDEKGGA"
     gene            complement(2658..3827)
                     /locus_tag="PK518_09555"
     CDS             complement(2658..3827)
                     /locus_tag="PK518_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRM49201.1"
                     /translation="MHETGLAPYACDPALSRGRRYFEPPAPTRTEYQRDRDRIVHSTA
                     FRRLVYKTQVFLNHEGDLFRTRLTHSLEVAQLGRSIARSLRLNEDLVEAICLAHDLGH
                     TPFGHAGQDALNDCMTGFGGFEHNLQSLRVVDALEDRYPAYDGINLTFETREGILKHC
                     SRRNAELIEAREPGGVGRRFLLGQQPSLEAQLCNLADEIAYNAHDIDDGVRSGLITLE
                     QLREVPLFEHYRAQAQAQYPQLGRATGRRRLLSEAIRRMLSDQVYDVIAATRAALAEH
                     APSDVDAVRGLPALVGFSAGMRAQSRQLKSFLFRELYRHPQVVQTTDGARQVVRELFA
                     LYQADPRQMPAAQAGWALEGDAQQRARTVADFIAGMTDRYAAREHERLTGQRLLG"
     gene            complement(3884..5005)
                     /gene="aroB"
                     /locus_tag="PK518_09560"
     CDS             complement(3884..5005)
                     /gene="aroB"
                     /locus_tag="PK518_09560"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRM49202.1"
                     /translation="MTQATLPATDTVRIALDERSYDIAIGADMLASLAQRADVPAGTA
                     ALVVSNTVVQPLYGAALAAALRQRYPVVHSVVLPDGEEHKIWQTLNLIFDALLSHACD
                     RKVVLYALGGGVIGDMTGFAAACYMRGVPFVQVPTTLLAQVDSSVGGKTAINHPLGKN
                     MIGAFYQPRLVLCDLATLDTLPARELAAGLAEVIKYGPIADMQLFAWLEQNMDALLAR
                     DRAALAHAVRRSCEIKAAVVGADERESGLRAILNFGHTFGHAIEAGMGYGVWLHGEGV
                     AAGMVMAAELSRRLGLVDAAFVQRLTDLIARAGLPTRGPVLDAADNAGRYLQLMRVDK
                     KAEAGEIRFVLIAGPGQAIMRAAPDALVREVIDHCCAIV"
     gene            complement(5135..6709)
                     /gene="pgi"
                     /locus_tag="PK518_09565"
     CDS             complement(5135..6709)
                     /gene="pgi"
                     /locus_tag="PK518_09565"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRM49203.1"
                     /translation="MHPRCDETPQWAQLQAHYQAQGRGLDLRRAFAEDAGRFAHFSQQ
                     APHVFADLSKNLWDRQAEALLLDMARACRLEHSRDAMFAGQAVNSTEGRAALHTLLRR
                     PRGLALPGDEPFVAQALQEVHATLDAMLAFAEAVRADAQITDIVNIGIGGSDLGPHMA
                     VRALQAHQLPGRRFHFVSNIDGHELASVLQAVRPESTLFIVASKTFTTLETMANARSA
                     LDWFAAQGGQDVARHFVALTANVAKAAEWGMSRTFGFWDWVGGRYSLWSAIGLPIAIA
                     IGAAGFRDFLAGAHAMDEHFRSAPLAHNLPVRLALLDIWYRNFLGLPSRCIAPYHAWL
                     QRLPAYLQQLEMESNGKRVDSQGRALPMATAPVIWGEPGSNGQHAFFQLLHQGPDVQA
                     LEIIAVRDSAHGLAGHHQMLLANALAQAQALMQGRASGCGHRHFPGNRPSSFVLLDAL
                     TPASLGAYLALQEHRVFCSGSLWGINSFDQWGVELGKQLAQDLQPRLRTGDVAGLDGS
                     TAGLLQRLAGVGGRAP"
     gene            complement(6717..7835)
                     /locus_tag="PK518_09570"
     CDS             complement(6717..7835)
                     /locus_tag="PK518_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRM49204.1"
                     /translation="MHSVVISGTGLVQPPHTITNAELVESFNRFVELDNARHADAIAA
                     GTRVALQPSSVEFIEKASGIKQRHVFDKRGILDPERMYPHYAERADGELSMMAEMAVA
                     AAGQALAQAGRSGADVDMVLCAASNMQRAYPAIAIEIQQALGAGGYGFDMNVACSSAT
                     FAIEQAVNAVRAGTARCVLVVNPEITSGHQAWTDRDCHFIFGDVCTALLIERTDLASN
                     PGAWEVLGTRLATQFSNNIRNNFGFLNRSEDSDPKARDKTFRQEGRKVFKEVVPIAAA
                     HIEGHLASLGLQPAGVRRFWLHQANEGMNQLVIKRLLGEAGRDRAPLILDEFANTASA
                     GSIIAFHQHRADLAAGDVGVVCSFGAGYSVGSVVVKKR"
     gene            complement(7887..8834)
                     /gene="tal"
                     /locus_tag="PK518_09575"
     CDS             complement(7887..8834)
                     /gene="tal"
                     /locus_tag="PK518_09575"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRM49205.1"
                     /translation="MNQLDALKQFTTVVADTGDFKQLAQFQPQDATTNPSLILKAVQK
                     PEYAPLLQQTVARWQGRGMDEVMDRLIVRFGCEILSIIPGRVSTEVDARLSFDSAATV
                     ARAERIVELYQAEGLHIDRVLIKIAATWEGIQAARQLEARGIHTNLTLLFSFAQAVAC
                     GQARVQLISPFVGRIYDWYKKHAGSAWDEAAMAGANDPGVQSVRAIYNHYKHFGIATE
                     VMGASFRNIGQIIALAGCDLLTIAPELLAQLAASDAPVQRVLDPEAARRVRLEPVQYD
                     EAGFRYALNADAMATEKLAEGIRAFAADAARLEQLIQAA"
     gene            8937..9782
                     /locus_tag="PK518_09580"
     CDS             8937..9782
                     /locus_tag="PK518_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM49206.1"
                     /translation="MLDRITASLPSLAPAEQRVAQLVLDDPRAFARLPVRELAARAQV
                     SKPTVVRFCRSMGYDGLADFKLKLAGSVSEGVPFIHRSVDADDKTGDVLVKVVDNAVA
                     AFLQYRNAASGNALERAAEAIVATWKTGRRIEFYGAGNSGIVAQDAQHKFFRLGVTSL
                     SSSDGHIQVMSATLLGPGDCAVIISNSGRTRDLMDAADIARKNGATTIAITASGSPLA
                     HSCHVHLAADHPEGYDRYSPMVSRLLHLLIIDVLATCVALRIGEPLQPVLQQMKNNLR
                     AKRYT"
     gene            complement(9916..10410)
                     /locus_tag="PK518_09585"
     CDS             complement(9916..10410)
                     /locus_tag="PK518_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRM49207.1"
                     /translation="MTTFTLSSPDIAAGASIDPSFEFDGFGCSGKNQSPVLRWSGAPV
                     GTKSFAVHVYDPDAPTGSGFWHWYVIDLPAGVTELAANAGAQGGANLPQGARQIRNDY
                     GSYSWGGCCPPPGDKPHRYIFTVHALSVDRIDVPDDAPAALTGFMVNANTLAKASFTA
                     TYGR"
     gene            complement(10463..11452)
                     /locus_tag="PK518_09590"
     CDS             complement(10463..11452)
                     /locus_tag="PK518_09590"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRM49208.1"
                     /translation="MQAHDLARTFDVSAPWLNRVIERKPRQLLHAVDGVSFEIEKGKT
                     LALVGESGCGKSTVARLLVGLYEPTRGGFTFDGQDAHAAFKGNEARAMRRRIQMIFQD
                     PYASLNPRWLVEDIIAEPLREHGLISDKAALKERVGQLLTSVGLAAADMAKYPHQFSG
                     GQRQRISIARALATEPEFLVCDEPTSALDVSVQAQVLNIMKDLQKKQGLTYLFISHNL
                     AVVRHVSDQVGVMYLGRLVELADKQQLFASPRHPYTRMLLDAIPKMHDTGRARTPVQG
                     EVPNPLNPPPGCAFNPRCPFANDRCRSERPQLLNQGGTRVACHAVEEGRISGA"
     gene            complement(11584..12558)
                     /locus_tag="PK518_09595"
     CDS             complement(11584..12558)
                     /locus_tag="PK518_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM49209.1"
                     /translation="MSLLEVQNLVVEFPGRRGTLRALDDVSFSIAPGEILGVVGESGA
                     GKSLTGASIIGLLEPPGRVASGAIRLEGQRIDNLGHEQMRHIRGRKIGAIFQDPLTSL
                     NPLYTVGQQLVETILTHLPMNAAQARQRAVELLKDTGIPAAEQRVDHYPHQFSGGMRQ
                     RVVIALALAAEPQLIVADEPTTALDVSIQAQIIGLLKTICKTRGAAVMLITHDMGVIA
                     ETCDRVAVMYAGRVAEIGPVHEVINHPAHPYTAGLMASIPDMEEERERLNQIDGAMPR
                     LNAIPKGCAFNPRCPKVFERCRQERPDLMSAGATQAACWLHAANKEAA"
     gene            complement(12564..>12808)
                     /locus_tag="PK518_09600"
     CDS             complement(12564..>12808)
                     /locus_tag="PK518_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM49210.1"
                     /translation="VLATIQVATAIITEATLSFLGVGAPPTSPSLGTLIRIGNDYLFS
                     GEWWITIFPGVMLVLIALSVNLLGDWLRDALNPRLR"
BASE COUNT         2063 a         4289 c         4358 g         2098 t
ORIGIN      
        1 tccaaaatct tcagcgcgcc ggtgacgatg tcgtggcgct tgacgccctt gatatgggcc
       61 acaaagccca ggccacaggc gtcatgctcg ttgtgggatg catacaggcc atgctgttgc
      121 aggtgctgga tctcggcagc cttggtcatc gcgcgctttc tcaaaatatc gcagggaagc
      181 ggagattaag gcatcaaccc atgcagcgca agcatattaa ttggggtcag attccaatta
      241 attttaggac atcatcgttt ccatttattt agggacatat taaattaata gctaataatg
      301 cttaattgat aagcattgca cctgttttgc tcagccggtt tgaatagcgg gacgcccagc
      361 tttgcccttg gcaacacggc gctgggtacg ttgttgcaac gcagcaatga agtccgcctc
      421 gcccagcgcc cagccggcat ggacggactg gccgatctgc gcctggtctg cccccacgat
      481 gcccgcccgc accagctcgc cataggccgc ctcgcgtgca aacggcgtgt tgcccagcga
      541 ccagaacagc gcatgcggcg tgaccagggg atcgttgcgc cgccccatgc tgtgcgcgta
      601 gctggaccag gggtagtcgc ccggctgggc gaccatgccg gcgcgcaccg ggttcaggtc
      661 gatgtagacc atgcaggcca gcaggtagcg ctcggactgc acgatggtcg aacggtagcg
      721 cccctcccac agcgtgccgc tgcgctggtg gcggcggttg aaatagcgta catagctgcg
      781 cccgacggac tgcatcatca gcggcaggcc atcctgcgtc tgcggcgtgg ccagcaggtg
      841 gaaatggttg cccatgagca cataggcgtg gatctcgacc tggagcttct tggcctgctc
      901 ggtcagcagg cccagcatga actcgtgatc cgcagcatcc ctgaagatca tctgccggtt
      961 gttgccacgc tgaatgacat ggtgcggata gccaggaacg gtgagccggg gcaggcgggc
     1021 catggagtct ccttaattgg ggtcagactc caattatcga gatggcaggc cgcctgtcaa
     1081 ttaattggaa tctgacccca ataaggtttg cacgccgaaa cgtgggccca gggcctgggt
     1141 gaggccaaac tcttccagga tggccgccag ccgctcgcgg gcgctgtgct tcttgtgacc
     1201 ggtgtggcgt gccaggatga gctcgttctt caggctgtgc tcccagccca ccaactcggt
     1261 gacggtgacc tggtagccgg aggcctccag gtgcaggcag cgcagcacat tggtgatctg
     1321 gctgccgagc tcgcgcgtgt ggatcgggtg gcgccacagc tcggccaggg gcgtgcgcgc
     1381 cagggacagg gccttggttt ggcgcaggct ggccgccacc tcggcctggc agcagggcac
     1441 gagcaccatg gcgcgggcct gtttttgcag gccgaaggca atcgcatcgt cggtggcggt
     1501 gtcgcaggcg tgcagcgcgg tgacgatgtc aaagcgctcg ggcaggccgg acagtgccgc
     1561 ggcgtcggcc acggccatat ccaggaactg catgcgctca aagcccaggc gctgcgccag
     1621 tgcgcgcgat cgctcgacca gctcggcgcg ccactctatg ccgtagatct cgccgcgccc
     1681 caggctcttg aagaacaggt cgtagaggat gaagcccagg taggacttgc ccgcgccatg
     1741 gtcggccagc agcggcgcat ggccatctgc cgacagctcg gccagcagcg gctctatgaa
     1801 ctggtacagg tggtagacct gcttgagctt gcgccgcgag tcctggttca gccgcccctc
     1861 gcgcgtgagg atgtgcagct ctttcagcag ctccacggac tggcccggcc gcaactcggc
     1921 cagcaccgcc gcctgggcgg cctgggccgc atcggccttg gactgggcgc ggctcatgcg
     1981 ccgccctttt catcatcctt cttgccgcca tggcccatgg ggcagcgcgc gcctacgtcc
     2041 agatcgaccc agccctgcat gcccagccgc tccagcacct cgatgttgcg ctcgaagatt
     2101 tcctcggcct cggggaacac ctccacggct cggtccaggc tttcctcgcg caacaggtgc
     2161 agcgtggggt acggcgcgcg attcgtgcag ttgctcacgt catcagccag cgtgccctcg
     2221 aactgaaagc gcgggtggaa cgacgccacc tgcaggatgc cggccagatc catggcctcg
     2281 accagctcgt cggccagctc caggaagtcg ttgaagtcca gaaagtccgg catcgccagc
     2341 ggcgccatca gcagcgtggt gtcgcgcttc tccgccggcg cctcggccag cgcctccagc
     2401 tcgcgctgta aatcctgtag cagctcgcgc gcatccgtgg cgtggctcac ggcgtaatgg
     2461 atctgatcct tggtgtgcac gcccttggca aacgggcaca gattcaggcc gatgacggcg
     2521 cgctccagcc agcgcacggt gtcctgcacc accacggcat cgtcgtagtc ggcggggttg
     2581 ggttgatctg gtgctgtggt catggcgtgg ctcgctccat aaggggcagg cattgtatgc
     2641 ctcgcctcag cgccggctca gcccagtaga cgctggcccg tcagccgctc atgctcgcgc
     2701 gcggcgtagc ggtcggtcat gccggcgatg aagtcggcga ctgtgcgcgc gcgctgctgc
     2761 gcgtcgccct ccagggccca gccggcctgc gccgccggca tctggcgcgg gtcggcctgg
     2821 tagagggcaa acagctcgcg caccacctgg cgcgcgccat cggtggtctg caccacctgg
     2881 gggtgacgat agagctcacg aaacagaaag cttttcagct gccgcgactg ggcacgcatg
     2941 ccggccgaga accccaccag cgccggcagg ccgcgcacgg cgtccacgtc ggagggcgca
     3001 tgttcggcca gggccgcgcg cgtggcagcg atcacgtcat acacctggtc gctgagcatg
     3061 cggcgtatcg cctcgctcaa tagccgacgc cggcccgtgg cccgccccaa ctgcgggtac
     3121 tgcgcctgcg cctgggcgcg gtagtgctcg aacagcggca cctcgcgcag ctgctccagg
     3181 gtgatcaggc cggagcgcac gccgtcgtcg atgtcgtgcg cgttgtaggc gatctcgtcg
     3241 gccaggttgc acagctgggc ctccaggctg ggttgctggc ccagcagaaa gcgccgcccc
     3301 accccgcccg gctcgcgcgc ctcgatcaac tcggcgttgc ggcgcgagca gtgcttcagg
     3361 atgccctcgc gcgtctcgaa cgtcaggttg atgccgtcgt acgccgggta gcggtcttcc
     3421 agcgcatcca ccacgcgcag gctctgcagg ttgtgctcga agccgccaaa gccggtcatg
     3481 cagtcgttca gcgcgtcctg cccggcgtgg ccgaagggcg tgtggcccag gtcatgcgcc
     3541 aggcagatgg cctcgaccag atcctcgttc aggcgcagcg agcgcgctat ggagcgcccc
     3601 agctgcgcca cctccagcga atgcgtgagc cgggtgcgaa acaaatcccc ctcgtggttc
     3661 aagaacacct gggtcttgta gaccaggcgc ctgaaggccg tggaatgcac gatgcggtcg
     3721 cggtcgcgct ggtactcggt gcgcgtgggc gccggcggct cgaagtaccg gcggccacgg
     3781 ctcaacgccg gatcacaggc gtagggggcc aggccggttt catgcatcaa aaaggcctcc
     3841 agcgctttac catcaaacgc aaacagctat tattaatata gcatcagacg attgcgcagc
     3901 aatggtctat gacctcgcgc accagcgcat cgggcgcggc gcgcatgatg gcctggcccg
     3961 gcccggctat gagcacaaag cggatctcgc cggcctcggc ctttttgtcc acgcgcatca
     4021 gctgcaggta gcggccggcg ttgtctgccg cgtccagcac cgggccgcgc gtgggcaggc
     4081 cggcgcgtgc aatcaaatcc gtcaggcgct gcacaaaggc cgcatcgacc agccccaggc
     4141 ggcgcgagag ctcggccgcc atcaccatgc ccgccgccac gccctcgcca tgcagccaga
     4201 cgccgtagcc catgccggcc tcgatggcgt ggccgaaggt gtggccaaag ttcaggatgg
     4261 cgcgcaggcc cgattcgcgc tcgtccgcgc cgaccaccgc cgccttgatc tcgcagctgc
     4321 gcctgacggc atgcgccagc gcggcgcgat cgcgcgccag cagcgcatcc atgttctgct
     4381 ccagccaggc aaagagctgc atgtcggcaa tcggcccata cttgatgacc tcggccaggc
     4441 cggcggccag ctcgcgcgcg ggcaaggtgt ccagcgtcgc caggtcgcac agcaccagcc
     4501 gcggctggta gaaggcgccg atcatgttct tgcccagcgg gtggttgatg gcggtcttgc
     4561 cacccaccga cgagtccacc tgcgccagca gcgtcgtcgg cacctgcaca aacggcacgc
     4621 cgcgcatata gcaggcggcg gcaaagcccg tcatgtcgcc aatcacgccg ccgcccaggg
     4681 cgtagagcac caccttgcgg tcgcaggcgt gggacagcag ggcgtcgaag atcaggttca
     4741 gggtctgcca gatcttgtgc tcctcgccgt cgggcaggac gacgctgtgc accaccgggt
     4801 agcgctggcg cagggccgcc gccagcgccg cgccatacag tggctgcact accgtgttgc
     4861 tcaccaccag cgccgccgtg ccggcgggca catcggcgcg ctgcgccagg ctggccagca
     4921 tatcggcgcc aatggctatg tcgtagctgc gctcgtccag tgcgatgcga acggtgtctg
     4981 tggccggcag ggtggcttgc gtcatgggtg gtggaatcgg gacgggttca aacaagggga
     5041 aaattatcgt tggcgccgcg cagagtgcta tttcatgccc caagcgcgca tgcagtaagc
     5101 gcgaacagct atcaaaacaa aagcatccag gctatcaggg cgccctgccg ccgacacccg
     5161 ccaggcgctg cagcaggccg gcggtggagc catccagccc cgccacatcg cccgtgcgca
     5221 ggcgcggctg caggtcctgc gccagctgct tgcccagctc cacgccccat tggtcaaagc
     5281 tgttgatgcc ccacaggctg ccgctgcaga acacgcggtg ctcctgcagc gccaggtagg
     5341 cgcccagcga cgcgggcgtg agggcgtcca gcagcacaaa gctgctcggc cggttgccgg
     5401 ggaaatgccg gtggccgcaa ccgctggcgc gcccctgcat cagcgcctgg gcctgggcca
     5461 gcgcattggc cagcagcatc tggtggtggc cggccaggcc gtgggcgctg tcgcgcacgg
     5521 cgatgatctc cagcgcctgc acgtcgggcc cctggtgcag cagctggaag aaggcatgct
     5581 ggccgttgct gccgggctcg ccccagatca cgggcgcggt ggccatgggc agggcgcggc
     5641 cctggctgtc cacgcgcttg ccgttgctct ccatctccag ctgttgcagg taggccggca
     5701 ggcgctgcag ccaggcgtgg tagggcgcga tgcagcggct gggcaggccc aggaagttgc
     5761 ggtaccagat gtccagcagc gccaggcgca cgggcaggtt gtgcgccagc ggcgcgctgc
     5821 ggaaatgctc gtccatggca tgcgccccgg ccaggaagtc gcggaaaccc gccgcgccta
     5881 tggcgatggc tatcggcaga cctatggccg accacagcga atagcgccca cccacccagt
     5941 cccagaagcc gaaggtgcgg ctcatgcccc actcggccgc cttggcgacg ttggccgtca
     6001 gcgccacgaa atgccgcgcc acatcctgcc cgccctgggc ggcaaaccag tccagcgccg
     6061 agcgcgcgtt ggccatggtc tccagcgtgg tgaaggtctt ggaggcgacg atgaacagcg
     6121 tgctctcggg ccgcacggcc tgcagcacgc tggccagctc atggccgtcg atgttggaga
     6181 cgaagtgaaa gcgccggccc ggcaactggt gggcctgcag cgcacgcacc gccatgtgcg
     6241 ggcccagatc ggagccgccg ataccgatgt tgacgatgtc ggtgatctgc gcgtcggcgc
     6301 gtacggcctc ggcaaaggcc aacatggcat ccagcgtggc gtgcacctcc tgcagggcct
     6361 gggcgacgaa gggctcgtca ccgggcaggg ccaggccccg ggggcggcgc agcagagtgt
     6421 gcagcgcggc gcggccctcg gtgctgttga cggcctggcc ggcgaacatg gcgtcgcggc
     6481 tgtgctccag ccggcaggcg cgcgccatgt ccagcagcag cgcctcggcc tgcctgtccc
     6541 acaggttctt ggacaggtcg gcaaacacat ggggcgcctg ctggctgaaa tgcgcgaagc
     6601 gccccgcgtc ctcggcaaag gcgcgccgca agtccaggcc acgcccttgg gcctggtaat
     6661 gcgcctgcag ctgcgcccac tgcggcgtct cgtcgcaacg cgggtgcatg gtggggtcag
     6721 cgctttttga caacgacact gcccaccgag tagcccgcgc caaacgagca gaccacgccc
     6781 acatcgcccg cggccaggtc ggcgcggtgc tggtggaagg caatgatgga gccggccgac
     6841 gcggtattgg caaactcatc caggatcagc ggcgcgcggt cacgccccgc ctcgcccagc
     6901 agccgcttga tgaccaactg gttcatgccc tcgttcgcct ggtgcagcca gaagcggcgc
     6961 acgccggcgg gctgcaggcc caggctggcc aggtgcccct cgatatgcgc ggcggcgatg
     7021 ggcaccacct ccttgaacac cttgcgcccc tcctggcgga aggtcttgtc gcgcgccttg
     7081 gggtcgctgt cctcgctgcg gttcagaaag ccgaagttgt tgcggatgtt gttggagaac
     7141 tgcgtcgcca ggcgcgtgcc cagcacctcc caggcgccgg ggttgctggc caggtcggtg
     7201 cgctcgatca gcagcgccgt gcacacgtcg ccaaagatga aatggcagtc gcgatccgtc
     7261 caggcctggt ggccggaggt gatctcgggg ttcaccacca gcacgcagcg ggccgtgccc
     7321 gcgcgcacgg cgttcaccgc ctgctctatg gcaaaggtgg ccgaggagca ggccacgttc
     7381 atgtcgaagc cgtagccgcc cgcgcccagc gcctgctgga tctcgatggc gatcgccggg
     7441 taggcgcgct gcatgttgga ggcggcgcag agcaccatat cgacatcggc cccgctcctg
     7501 ccggcctggg ccagcgcctg gccggcggcg gccacggcca tctcggccat catcgacagc
     7561 tcgccgtcgg cgcgctcggc gtaatgcggg tacatgcgct cggggtcgag aatgccgcgc
     7621 ttgtcgaaca catggcgctg cttgatgccg gaggccttct cgatgaactc gacgctggac
     7681 ggctgcaggg ccacacgcgt gccggcggca atggcgtcgg cgtggcgcgc gttgtccagt
     7741 tccacgaagc ggttgaagga ctccaccagc tcggcgttgg tgatggtgtg cggcggctgg
     7801 accaggccag tgccgctgat gacgacggaa tgcataggtt cgtgtcccat gcccatgcga
     7861 ctgggctggt gtggcaagtg ttgcgatcaa gcggcctgga tcagctgctc cagcctggcg
     7921 gcgtcggcgg cgaaggcgcg aatgccctcg gccagcttct cggtggccat ggcgtcggcg
     7981 ttgagcgcgt agcggaaccc ggcctcgtcg tactgcaccg gctccaagcg cacgcggcgc
     8041 gcggcctcgg ggtcaagcac acgctgcacc ggcgcatcac tggccgccag ctgggccagc
     8101 agctcggggg cgatggtcag caggtcgcag ccggccagcg cgatgatctg gccgatgttg
     8161 cgaaagctcg cccccatgac ctcggtggcg atgccgaaat gcttgtagtg gttgtagatg
     8221 gcgcgcacgg actgcacgcc ggggtcgttg gcgccggcca tggccgcctc atcccaggcg
     8281 ctgccggcat gcttcttgta ccagtcgtag atgcggccga caaagggcga gatcagctgc
     8341 accctggcct ggccgcaggc caccgcctgg gcgaaggaga acagcagcgt caggttggtg
     8401 tgtatgccgc gcgcctccag ctggcgcgcc gcctgtatgc cctcccaggt ggcggcgatc
     8461 ttgatcagca cgcggtcgat atgcagccct tcagcctggt acagctccac gatgcgctcg
     8521 gcgcgcgcca cggtggcggc gctgtcgaag gacaggcgcg catcgacctc ggtggacacg
     8581 cgcccgggga tgatggacag gatctcgcag ccaaagcgca cgatgagtct gtccatgacc
     8641 tcatccatgc cccggccctg ccagcgcgcc accgtctgct gcagcagcgg cgcgtattcg
     8701 ggtttttgca ccgccttcag gatcagcgag ggattggtcg tcgcatcctg cggctggaac
     8761 tgcgccagct gcttgaagtc gccggtatcg gccaccacgg tggtgaattg cttgagtgcg
     8821 tcgagttggt tcatgcgagt ctttggtagc cagagcctca agtgtatgcg gggcgcaggc
     8881 catgaaaccc ggttacattg cgcaggcacc cactcccacc cattcccgcc cattccatgc
     8941 tggatcgcat caccgcctcc ctgccctccc tggcgcccgc agagcagcgc gtggcccagc
     9001 tcgtgctgga cgacccgcgc gccttcgccc gtctgccggt gcgcgagctg gccgcgcgcg
     9061 cccaggtcag caagcccacg gtggtgcgct tttgccgcag catgggctat gacgggctgg
     9121 ccgacttcaa gctcaagctg gccggcagcg tgagcgaggg cgtgcccttc atccaccgca
     9181 gcgtggacgc cgacgacaag accggcgacg tgctggtgaa ggtggtggac aacgccgtgg
     9241 ccgccttcct gcagtaccgc aacgccgcca gcggcaacgc cctggagcgc gccgccgagg
     9301 ccatcgtcgc cacctggaag accggccggc gcatcgagtt ctacggcgcc ggcaactccg
     9361 gcatcgtggc ccaggacgcg cagcacaagt tctttcgctt aggggtcacc agcctgtcca
     9421 gcagcgacgg ccatatccag gtcatgagcg ccacgctgct cggcccgggc gactgcgcgg
     9481 tgatcatcag caactccggc cgcacgcgcg acctgatgga cgcggccgac atcgcgcgca
     9541 agaacggcgc cacgaccatc gccatcaccg ccagcggctc gcccctggcg cacagctgcc
     9601 acgtccacct ggccgccgac caccccgagg gctacgaccg ctacagcccc atggtctcgc
     9661 gcctgttgca cctgctcatc atcgacgtgc tggccacctg cgtggcgctg cgcattggcg
     9721 agccgctgca gccggtgctg cagcagatga agaacaatct gcgcgcgaaa cgctacacgt
     9781 aaaaacaata gctgtcagcg cttgatgggt aagcgtttga cagcaatttg agcagatttt
     9841 tagtgctgaa ctggagcgcc ggcgtctcgc cggcataccg ctccaggaga gccggcgaga
     9901 cgccggcgct cctgctcaac gcccgtaggt cgccgtgaag gaagccttgg ccagggtgtt
     9961 ggcattgacc atgaagccgg tcagcgcggc gggcgcgtcg tcgggcacgt caatgcgatc
    10021 caccgacagc gcatgcacgg tgaagatgta gcggtgcggc ttgtcgccgg gcggcgggca
    10081 gcagccgccc caggagtagc tgccgtagtc attgcggatc tggcgcgcgc cctggggcag
    10141 gttggcgccg ccctgggcgc cggcgttggc ggccagctcg gtcacgccgg ctggcaggtc
    10201 gatcacatac cagtgccaga agcccgagcc cgtgggcgcg tccgggtcgt agacatgcac
    10261 ggcgaagctc ttcgtgccca cgggggcgcc actccagcgc agcacgggcg actggttctt
    10321 gccgctgcag ccaaagccgt cgaactcgaa gctcgggtca atgctggcgc cggcggcaat
    10381 gtcggggctg ctgagggtga aggtggtcat gggcaatgct cctttgctgt gatgaacatg
    10441 aggcccgcag tatgccccgc cgctacgccc ccgaaatgcg cccctcctcg acggcatggc
    10501 aggccacgcg tgtgccgccc tggttcaaca gctgcgggcg ctcgctacgg cagcggtcgt
    10561 tggcaaacgg gcagcgcggg ttgaaggcgc agccgggcgg cgggttgagt ggattgggca
    10621 cctcgccctg cacgggcgtg cgcgcgcggc cggtgtcatg catcttgggg atggcgtcca
    10681 gcagcatgcg cgtgtacggg tggcgcgggc tggcaaacag ctgctgcttg tcggccagct
    10741 ccaccaggcg gcccaggtac atcacgccga cctggtcgct cacatggcgc accacggcca
    10801 ggttgtggct gatgaacaga taggtcaggc cctgcttctt ctgcaggtcc ttcatgatgt
    10861 tcagcacctg ggcctgcacc gatacgtcca gggcgctggt gggttcgtcg cagaccagaa
    10921 attcgggctc ggtggccagg gcgcgtgcga tggagatgcg ctggcgctgc ccgcccgaga
    10981 actggtgcgg gtacttggcc atgtcggccg cggccaggcc cacggaagtg agcagctggc
    11041 ccacgcgctc cttgagcgcg gccttgtcgc tgatcaggcc atgctcgcgc agcggctcgg
    11101 cgatgatgtc ttcgaccagc cagcgcgggt tcaggctggc atacgggtcc tggaaaatca
    11161 tctgtatgcg ccggcgcatg gcgcgtgctt cgttcccctt gaaggccgcg tgcgcatcct
    11221 ggccgtcaaa cgtgaagccg ccgcgcgtgg gctcgtacag gccgaccagc aggcgcgcca
    11281 ccgtgctctt gccgcagccg gattcaccca ccagggccag ggtcttgccc ttttcgatct
    11341 cgaagctcac gccgtccacg gcgtgcagca gctggcgcgg cttgcgctcg atcacccggt
    11401 tgagccaggg ggcggagacg tcgaaggtgc gcgccaggtc gtgcgcctgc accagcggct
    11461 ttacctctcc cgcccccttt gggggagggt tggggtgggg gccggcaccg cccgcgccct
    11521 gctcaacgag gctggccccc atcccggcct tcccccagag ggggaaggag acattgcccg
    11581 tgctcatgcg gcctccttgt tcgcggcgtg cagccagcag gcggcctggg tggcgcctgc
    11641 gctcatcagg tcgggccgct cctggcggca gcgctcaaag actttcgggc agcgcgggtt
    11701 gaaggcgcag cccttcggga tggcgttcag gcgcggcatg gcgccgtcga tctggttcag
    11761 gcgctcgcgc tcctcctcca tgtcggggat ggaggccatg aggccggcgg tatagggatg
    11821 ggcggggtgg ttgatgacct cgtgcacggg tccgatctcg gccacgcggc cggcatacat
    11881 cacggccacg cggtcgcagg tctcggcgat cacgcccatg tcgtgtgtga tcagcatcac
    11941 ggccgcgccg cgtgtcttgc agatggtctt caagagccca atgatctgcg cctggatgga
    12001 cacgtccagc gccgtggtcg gctcgtcggc cacgatgagc tgcggctcgg ccgccagcgc
    12061 cagggcgatc accacgcgct ggcgcatgcc gcccgagaac tggtgcgggt agtgatcgac
    12121 gcgctgctcg gccgcaggga tgccggtgtc cttgagcagc tccacggcgc gctggcgcgc
    12181 ctgcgcggcg ttcatgggca ggtgggtcaa tatggtctcg accagctgct ggcccacggt
    12241 gtacagcggg ttcagcgagg tcagcggatc ctggaagatg gcccctatct tgcgcccgcg
    12301 gatatggcgc atctgctcgt ggcccaggtt gtctatgcgc tgaccctcca accggatcgc
    12361 ccctgaggcg acgcgccccg gcggttccag caggccgatg atggaggcgc cggtgagcga
    12421 cttgcccgcg ccggattcac ccaccacgcc caggatctcg cccggcgcga tggagaacga
    12481 cacatcgtcc agcgcccgca gcgtgccgcg gcggccgggg aattcaacga ccaggttttg
    12541 tacttcgagt aaggacatag gcttcaacgc aaacggggat tgagcgcatc acgcagccag
    12601 tcgcccagca ggttcaccga cagggcgatc agcaccagca tcacgccggg gaagatggtg
    12661 atccaccact cgcccgaaaa caaatagtcg ttgccgatgc ggatcagcgt gcccagcgac
    12721 ggcgaggtgg gtggcgcgcc cacgcccagg aacgacagcg tggcctcggt gatgatggcc
    12781 gtggccacct ggatggtggc cagcacca
//
LOCUS       DAOMTQ010000087        12455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_900_length_12455_cov_26.479516, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000087 DAOMTQ010000000
VERSION     DAOMTQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12455
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_900_length_12455_cov_26.479516"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..547)
                     /locus_tag="PK518_09605"
     CDS             complement(128..547)
                     /locus_tag="PK518_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRM49211.1"
                     /translation="MTEQTESLPPVPEQPPLPALARWLLFVFAVLCLALGVIGVIVPG
                     LPSTVFVLMAAWAAARSSPRLYRWLWFHPLFGSMLRNWANGGRVSRHAKWSASVLMTL
                     CAIILLVIDTPRWAAYSGCGFMACVLTWLWLRPEPEA"
     gene            635..1174
                     /gene="def"
                     /locus_tag="PK518_09610"
     CDS             635..1174
                     /gene="def"
                     /locus_tag="PK518_09610"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRM49212.1"
                     /translation="MTVRTVLKMGDPRLLRVARPVTAFDTQELHQLLTDLLDTMQAAN
                     GAGLAAPQIGVDLAVVVFGSGLPNPRYPDAPIVPRTVLVNPVITPIGDAEQLDWEGCL
                     SVPGLRGLVPRWQSVRYTGFGVRGEPIDRTVSGFHARVVQHECDHLWGKLYPMRMRDF
                     SQFGFTEVLFPDIGAAEEE"
     gene            1364..2833
                     /locus_tag="PK518_09615"
     CDS             1364..2833
                     /locus_tag="PK518_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HRM49213.1"
                     /translation="MNTNSTVPASELRPEDEKLGLGANLAYGLQHVLTMYGGIVAVPL
                     IVAEAAGMSAADAGLLITACLFMGGVATLLQTLGIPFFGSRLPLVQGVSFAGVATMVS
                     ILQTGGGLPGILGAVMASAVLGLLVAPVFSKVTRFFPPLVNGCVITVIGLSLIPVAAH
                     WAMGGNARAADYGSMSNIALAGISLVIVLGLSKVGSAAISRLSILLSIVLGTVVAVFM
                     GKADFSRVLEGAWFAIPTPMHFGMPTFNTAAILSMFIVILVILVETSADVLAVGDIVG
                     TRVDSRRLGDGLRADMLSSIVAPVFGAFTQSAFAQNVGLVVVTGVKSRYVVAYSGLIL
                     IALGVLPIMGRVVACVPPSVLGGAGLVLFGTVAASGIRTLSKVDYNNNMNLIVVAAAV
                     GAGLIPVVAPKFYAQFPDWFATIFHSGISATALVAILLNLLFNHFKQGNSDQQSVFVA
                     ASDRTLSAQVLASLYDGDVVIDGRLYDKNGKLVPVASAH"
     gene            complement(2920..5544)
                     /locus_tag="PK518_09620"
     CDS             complement(2920..5544)
                     /locus_tag="PK518_09620"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRM49214.1"
                     /translation="MPSRPSPDTAPLQALQALRDGYRQDKAALLDKLQAAGASTRGIR
                     KLLLKLSHLTDRLLCRLWAGARLPQDWALVAVGGYGRAQLFPHSDVDVLLLLPDGTAA
                     ALDDARKPIEAFISSCWDAGLEIGSSVRSVAECLSESAADVTVQTALLEARLLCGSPA
                     LFDEFCRRYDAQMDARAFLQAKTLEMRQRHTKYEDTPYALEPNCKESPGGLRDLQLII
                     WAANAAGLGSSWRELAANGLATPFELRQIERNEALLLLIRARLHAIAGRREDRLVFDL
                     QTAVAESFGYRSTAPDGQRLAMRASETLMRRYYWAAKAVTQLSQILLLGIEERLNPST
                     HELRPINARFFDKAGLIEVASDDLYERHPHAILETFLLYQSSPALKDLSARTLRALYN
                     ARGLMDAGFRRDPANRAQFMQILRQPSGITHAMRLMNQTSVLGRYLWPFRRIVGQMQH
                     DLFHVYTVDQHILMVLRNMRRFFMAEHAHEYPFCSQLASGWDKPWVLYLAALFHDIGK
                     GRGGNHSQIGAQEVRRFCRQHGIDAADAQLAEFLVREHLLMSQVAQKQDLSDPDVIRA
                     FAQHVGNERQLTALYLLTVADIRGTSPKVWNAWKGKLLEDLYRSTLRVLGGRAPDPAA
                     EIEARKREALILLALSALPHEAHKRLWDTLDVSYFMRHEAPDIAWHTRHLSRHVGAAR
                     AIVRARLSLAGEGLEVLVYAPDQADLFARICGYFDRAGFSILDARVHTAHNGHALDTF
                     QVVATSQPGAYRELIHMVENDLQRAIEDRGALPEPARKRVSRRVRSFPIAPRVTLRPD
                     EKAQRWLLSISASDRAGLLYLVARVLSRHSLSVQLAKISTLGERVEDTFLLQGAELQS
                     NQQQIRIETELLQALADQ"
     gene            complement(5588..6406)
                     /gene="map"
                     /locus_tag="PK518_09625"
     CDS             complement(5588..6406)
                     /gene="map"
                     /locus_tag="PK518_09625"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRM49215.1"
                     /translation="MSNITIKDAQGIAGMRQACRLASEVLDYIAPHIKPGITTLEIDR
                     LGAECMAQQGTRSATVGYQPPGYPPYPGHLCTSVNHVVCHGIPNDKPLKKGDIVNVDV
                     TVITPDGWYGDNSRMYLIGECSIAAKRLSALTFDAMWLGIQQVRPGARLGDIGHAIQK
                     YAEGHGLSVVREFCGHGIGQKFHEDPQVLHYGRPGTGQELAPGMTFTIEPMLNLGKRD
                     IKELGNDGWTIITKDRSLSAQWEHTVLVTETGYDVLTLSAGSPALPSFVTATAT"
     gene            6595..10629
                     /gene="purL"
                     /locus_tag="PK518_09630"
                     /gene_synonym="purI"
     CDS             6595..10629
                     /gene="purL"
                     /locus_tag="PK518_09630"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRM49216.1"
                     /translation="MTLHITQLEGGNALSSFRAQQLLPQLQAIHPKIEGLAARHVHLV
                     ARDAAPSEAERERLAALLNYGDPYAGPADGLALLVTPRLGTVSPWASKATDIAHNCGL
                     QLRRVERITEYRISLKGGLLGGRPELTPEQLQQVAALLHDRMTESVVATRAEAQELFT
                     ELDAEPMAFVDVLGGGRAALEAANKAWGLALADDEIDYLVNAFKGLARNPTDVELMMF
                     AQANSEHCRHKIFNAQFTIDGVAQDKSLFGMIRHTEAVSPQHTIVAYADNASIMEGSA
                     VEQFFAKFEAFAGQASATSYQKRSGTHHVLMKVETHNHPTAISPFPGAATGAGGEIRD
                     EGATGRGSEPKAGLTGFTVSKLWGSQVGKPEHIASPLQIMTEGPLGGAAFNNEFGRPN
                     LCGYFREYEQQVGGVTRGYHKPIMIAGGLGTIDAQHTKKILFPAGTLLIQLGGPGMRI
                     GMGGGAASSMATGTNAAELDFDSVQRGNPEIERRAQEVINHCWAQGADNPILAIHDVG
                     AGGLSNAFPELTNDAGRGARFDLRAVPLEESGLAPKEIWSNESQERYVLAIAPESLAQ
                     FTAFCERERCPFAVVGVATAERQLLVGDIDLHHAERSTPHAQLAVDMPMDVLLGKPPK
                     MHRDVQTVQRSFAPIQLDGLALEKAVIDVLAHPTVASKRFLINIGDRAVGGLTHRDQM
                     VGPWQVPVADVAVTLADYKGFAGEAMAMGERTPLAVINAPASGRMAVAEAITNLLAAP
                     IELPRVKLSANWMAACGEPGEDADLYATVKAVGMELCPQLGISIPVGKDSLSMRTQWQ
                     EGGQTKKVTSPVSLIITAFATLADVRGTLTPQLDAGEEDSTLVLIDLGRGKNRMGGSI
                     LGQVLGQSGNETPDLDDAKDLIALVNAVNALRAEGKILAYHDKGDGGLLATVAEMAFA
                     GQLGVALNVDMLITEGDGISDSRMDSGEGKNWGQQVSGRREAQTLAALFNEELGVVLQ
                     IRTADRSAVMQTLREHGLIQCSHTIGKTRPLASPVDAGKGELQVWRDAKKVFGASLSD
                     LHQVWDSVSWKICQQRDNPACADSEHAAMGDPSNPGLHVQLTFDPLDDVAAPFVNLAR
                     PKVAVLREQGVNSHVEMAYAFTEAGFEAFDVHMTDLQAGRARLSDFAGVVACGGFSYG
                     DTLGAGIGWARSITFNPALSEQFQQFFGRGDTFALGVCNGCQMFAELADIIPGAQAWP
                     RFTTNQSHRFEARLSMVEVLASPSLFLQGMAGSRLPIVVSHGEGYANFERRGNADKVV
                     PAMRFVNNHGQATEQYPFNPNGSPGGLTAVTTQDGRFTAMMPHPERAFRNIQMSWTDL
                     AATGGQDTLSPWMRIWRNARKWVG"
     gene            10727..11716
                     /locus_tag="PK518_09635"
     CDS             10727..11716
                     /locus_tag="PK518_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRM49217.1"
                     /translation="MAGASLLTLLDDIAAILDDVALMSQMAAKKSAAMADDVAVMTKV
                     AGQKTAGVLGDDLALNAQQVTGVRAEREIPVVWAVAIGSLVNKAILVPAALAISAWAP
                     WLVTPLLMLGGAFLCFEGVEKLAHKYLHSAAADAAAHASHAQANADPAIDLVAMERGK
                     IKGAVRTDFILSAEIIVIALGTVAAAPFAQQVAVLVSIALLMTVGVYGLVAGIVKLDD
                     LGLWLTGKASALARALGRALLAAAPRLMKFLSVAGMAAMFLVGGGILVHGVPALHHAI
                     DGLGLAAAAWPLGGLWSLLTTNLLNAVVGLAAGAAVLSAWLLLGRLRGSAVAH"
     gene            11868..12455
                     /locus_tag="PK518_09640"
     CDS             11868..12455
                     /locus_tag="PK518_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family protein"
                     /protein_id="HRM49218.1"
                     /translation="MKKNLLALAILCATTAGAAHAQQDGPWLVRARAVHLASDNGGAA
                     GAAGVSINDKWIPEVDVSYFFTPNFAAELILTYPQKHDVRLNGGKIGSVKHLPPTLLA
                     QYHFTGLGAFKPYVGAGINYTRFSGVDIAGGALTVKKNSWGPALQLGFDYALDKHWSL
                     NLDVKKVYIDTTVSGGVGRFKVDPVLVGLGVGYRF"
BASE COUNT         2040 a         4074 c         4239 g         2102 t
ORIGIN      
        1 ctcgaaccag ggacctacgg attaacagtc cggcgctcta ccgactgagc tatcggggaa
       61 caagccttag attatataca taaaaatcag catgcctggc agaaatttac cgctcggagc
      121 aggcgcgtca ggcctcgggc tcggggcgca gccacagcca ggtgagtaca caggccatga
      181 agccgcagcc cgagtaggcg gcccagcgcg gcgtgtcgat caccagcagg atgatggcgc
      241 acagcgtcat caggacggag gcgctccatt tggcgtggcg gctcacacgc ccgccattgg
      301 cccagttgcg cagcatggag ccgaacagtg ggtggaacca cagccagcgg tacaggcgcg
      361 gtgagctgcg cgccgccgcc caggcggcca tgagcacgaa aaccgtgctc ggcaacccgg
      421 gcacgatgac ccctatcacg cccagggcca ggcacagcac ggcaaacacg aacagcagcc
      481 agcgtgccag cgccggcagg ggcggctgct cgggcacggg tggcaaggac tcggtctgct
      541 cggtcatgct ggcgattgtg cccggggcgg tacggggcat gacaagcgtt atcctcgata
      601 gcctccatgc caccatagcc tttgacttgc ctcaatgacc gttcgcaccg tattgaagat
      661 gggcgacccg cgcctgttgc gcgtggcccg gcccgtgaca gcctttgaca cgcaggagct
      721 gcaccagctg ctcaccgacc tgctggacac catgcaagcc gccaatggcg ccgggctggc
      781 cgcgccccag atcggcgtcg atctggcggt ggtcgtattt ggctcgggtc tgcctaaccc
      841 gcgctacccg gacgcgccca tcgtgccgcg cacggtgctg gtcaacccgg tgatcacgcc
      901 catcggcgat gccgagcagc tcgactggga gggctgcctg tcggtgcctg gcctgcgtgg
      961 actggtgccg cgctggcaga gcgtgcgcta caccggcttt ggcgtgcgcg gcgagcccat
     1021 tgaccgtacg gtgagcggct ttcacgcacg cgtggtgcag catgaatgcg accatttgtg
     1081 gggcaagctt tatcccatgc gcatgcggga cttcagtcag ttcggcttta ccgaggtgct
     1141 gtttccggac ataggcgcgg ccgaggagga gtgacttggg cgcatgaatg atgcgctcca
     1201 cggcaaagga ctgtatacat atcggcatgc aatttttgtc gactgtgatt ctaaagttcg
     1261 ctgcaggccg tggctgggga gggtcttcat gcagggcagc acagcacggc acggcacaag
     1321 aggccaccaa gaggcccagg caatggattc aaggagacat acggtgaaca cgaattccac
     1381 agtcccggcc agcgaactgc gcccggagga tgaaaaactg ggcctgggcg ccaatctggc
     1441 ctatggcctg cagcatgtgc tgaccatgta tggcggcatc gtcgccgtgc cgctgattgt
     1501 ggccgaggcg gctggcatgt cggccgccga tgccggtctg ctgatcaccg cctgcctgtt
     1561 catgggcggc gtggcgacgc tgttgcagac cctgggcatt ccgttttttg gcagccgcct
     1621 gccgctggtg caaggcgtgt cgtttgccgg cgtggccacc atggtcagca tcttgcagac
     1681 cggcggcggg ctgccgggca ttctgggcgc ggtgatggcc tcggccgtgc tgggcctgct
     1741 ggtcgctccg gtgttctcca aggtcacgcg cttcttcccg ccgctggtca acggctgcgt
     1801 gatcacggtg attggcctgt cgctgatccc cgtggccgcg cactgggcca tgggcggcaa
     1861 cgcccgcgcc gccgattacg gcagcatgag caatattgcg ctggcgggca tttcgctggt
     1921 catcgtgctg ggtttgagca aggtgggcag cgcggccatc tcgcgcctgt cgattctgct
     1981 gtccatcgtg ctgggcacgg tggtggccgt gttcatgggc aaggccgact tctcgcgcgt
     2041 gctggagggt gcctggttcg ccattcccac gcccatgcac tttggcatgc cgaccttcaa
     2101 cacggcggcg attctgtcga tgttcatcgt catcctggtg atcctggtgg aaacctcggc
     2161 cgatgtgctg gccgtgggcg acatcgtggg cacgcgcgtg gattcgcgcc gcctgggcga
     2221 cggcctgcgc gccgacatgc tgtcgagcat cgtggccccg gtgtttggcg cgttcacgca
     2281 gagtgcgttc gcgcagaacg tgggcctggt ggtggtcacc ggcgtcaaga gccgctacgt
     2341 cgtggcctac agcggtttga ttctgattgc cctgggggtg ttgcccatca tgggccgggt
     2401 ggtggcctgc gtgccgccct cggtgctggg cggggccggc ctggtgttgt ttggcaccgt
     2461 ggcggccagt ggcatacgca ccttgtccaa ggtggactac aacaacaaca tgaacctgat
     2521 cgtggtggcg gcggcggtag gcgcgggcct gattccggtg gtggcgccca agttctatgc
     2581 gcagtttccg gactggtttg ccaccatatt ccactcgggc atcagcgcca cggccctggt
     2641 cgccatcttg ctgaacctgt tgttcaacca tttcaagcag ggcaactcgg atcagcaatc
     2701 ggtgtttgtc gcggcgtcag accgcacgct cagcgcgcag gtgctggcca gcctgtacga
     2761 cggagatgtg gtgatagacg gcaggctcta tgacaagaat ggcaagctgg tgcccgtggc
     2821 cagcgcccat tgacaggcgc agccagggct atggcgattt aatataaaac aagcctctag
     2881 cgcttatctg gcaagtgcaa acagctataa atagcatagc tattgatcgg ccaaggcctg
     2941 cagcagctcc gtctcgatgc ggatctgctg ctggttgctt tgcagttctg cgccctgcag
     3001 caggaaggtg tcttccacgc gctcgcccag ggtgctgatc ttggccagct gcacgcttaa
     3061 agagtggcgc gagagcacgc gtgccaccag gtacagcagg ccggcgcggt cgctcgccga
     3121 gatgctgagc agccagcgct gggccttttc gtcggggcgc agcgtgacgc gtggcgcgat
     3181 cggaaagctc ctgacccggc gcgacacgcg cttgcgcgcg ggctcgggca gcgcgccccg
     3241 gtcctcgatg gcgcgctgta ggtcgttctc gaccatgtgg atgagttcgc ggtaggcgcc
     3301 gggctggctg gtggcgacga cctggaaggt gtccagcgca tggccgttgt gcgcggtgtg
     3361 cacgcgcgcg tccaggatgg agaagcccgc gcggtcgaag tagccgcaga tgcgggcgaa
     3421 caggtcggcc tggtcgggcg cgtagaccag cacctccagc ccctcgccgg ccagcgacag
     3481 ccgggcgcgc acgatggccc gggcagcgcc cacatggcgc gagaggtggc gcgtgtgcca
     3541 ggcgatgtcg ggcgcctcgt ggcgcatgaa gtagctcacg tccagcgtgt cccacagccg
     3601 cttgtgggcc tcgtgcggca gggcgctcag ggccagcagg atcagcgcct cgcgcttgcg
     3661 tgcctcgatc tcggcggccg ggtcgggcgc gcgcccgccc agcacgcgca gcgtggagcg
     3721 gtacaggtct tcgagcagct tgcccttcca ggcgttccag accttgggcg aggtgccacg
     3781 tatgtcggcc accgtgagca gatacagcgc cgtgagctgg cgctcgttgc ccacatgctg
     3841 ggcaaaggcg cggatcacgt cggggtcgga caaatcctgc ttttgcgcca cctggctcat
     3901 caggagatgc tcacgcacca aaaattcggc cagctgcgcg tcggccgcgt cgatgccatg
     3961 ctggcggcaa aagcgccgca cctcctgcgc gccgatctgc gagtggttgc cgccgcggcc
     4021 cttgccgatg tcgtggaaca gcgccgcgag gtacagcacc cagggcttgt cccagccgct
     4081 ggccagttgc gagcagaacg ggtattcgtg cgcatgttcg gccataaaga agcggcgcat
     4141 gttgcgcaac accatgagga tgtgctgatc caccgtgtag acatggaaca ggtcgtgctg
     4201 catctggccg acgatgcgcc tgaagggcca gaggtagcgc cccagcaccg aggtctggtt
     4261 catcagccgc atggcgtgcg tgatgcccga gggctggcgc agtatctgca tgaactgcgc
     4321 gcggttggcc gggtcgcgcc gaaaaccggc gtccatcagg ccgcgcgcgt tgtacagcgc
     4381 gcgcagggtg cgcgccgaca ggtccttgag cgccggcgag ctctggtaga gcaaaaaggt
     4441 ctccaggatg gcgtgcgggt ggcgctcgta caggtcgtcg ctggccacct cgatcaggcc
     4501 ggccttgtcg aaaaaacgcg cgttgatggg gcgcagctca tgcgtgctgg ggttgagccg
     4561 ctcctctatg cccaacagca ggatctggct gagctgcgtg acggccttgg ccgcccagta
     4621 gtagcggcgc atcagcgtct cgctggcgcg catggccagg cgctggccgt cgggggcggt
     4681 ggagcggtag ccaaagctct cggccacggc ggtctgcaga tcgaacacca ggcggtcctc
     4741 gcgccgcccg gcaatggcgt gcaggcgggc gcggatcagc agcagcaggg cctcgttgcg
     4801 ctcaatctgc cgcagctcga agggcgtggc caggccgttg gcggccagct cgcgccagct
     4861 gctgcccagc cccgcggcgt ttgccgccca gatgatgagc tgcaggtcgc gcaggccgcc
     4921 gggcgattcc ttgcagttgg gctccagcgc gtagggcgtg tcctcgtact tggtgtggcg
     4981 ctggcgcatc tccagcgtct tggcctgcag aaaggcgcgc gcgtccatct gggcgtcgta
     5041 gcgccggcaa aattcgtcga acaatgccgg gctgccgcac agcaggcgcg cttccagcag
     5101 ggccgtctgc accgtcacat cggcggcgga ctcgctcagg cattcggcca cgctgcgcac
     5161 gctggagcct atctccaggc ctgcgtccca gcagctgctg atgaaggcct cgatgggttt
     5221 gcgcgcgtca tcgagcgccg cggccgtgcc atcgggcagc agcagcagca cgtccacgtc
     5281 tgaatgcgga aacagctgcg cgcgcccata gccgcccacc gctaccagcg cccagtcctg
     5341 cggcaagcgc gcgcccgccc acagccggca cagcagccgg tcggtcaggt gcgacagctt
     5401 cagcagcagc ttgcgaatgc cgcgcgtgga ggctccggcc gcctgcagct tgtccagcag
     5461 ggcggccttg tcctggcggt agccatcgcg tagcgcttgc agcgcctgca gcggcgcggt
     5521 gtccgggctc gggcgtgatg gcatgagaag gcttgccgtg aaggtcgttg ccgccagccc
     5581 cgggccatca ggtggcggtg gccgtcacaa acgagggcag ggcgggcgag cccgccgaca
     5641 gcgtgagcac gtcgtagccc gtttccgtga ccagcaccgt gtgctcccac tgggccgaga
     5701 ggctgcggtc cttggtgatg atggtccagc cgtcgttgcc cagctccttg atgtcgcgct
     5761 tgcccaggtt cagcatgggc tcgatggtga aggtcatgcc gggcgccagc tcctggcccg
     5821 tgccggggcg gccgtagtgc agcacctgcg ggtcttcgtg gaacttctga ccaatgccgt
     5881 ggccgcagaa ctcgcgcacc accgacaggc catggccctc ggcgtatttc tggatggcat
     5941 ggccgatgtc gcccaggcgc gcgccgggcc tgacctgctg aatgcccagc cacatggcgt
     6001 cgaaggtcag ggccgacagc cgcttggcgg caatcgagca ttcgcctatc aggtacatgc
     6061 ggctgttgtc accgtaccag ccgtccgggg tgatcacggt cacgtccacg ttgacgatgt
     6121 cgcccttctt caggggcttg tcgttgggta tgccgtggca caccacatgg ttgaccgagg
     6181 tgcacaaatg gccggggtag ggcgggtagc cgggcggctg gtagcccacg gtggcggacc
     6241 tggtgccctg ctgcgccatg cattcggcgc ccaggcggtc gatctcgagg gtggtgatgc
     6301 cgggcttgat atgcggtgcg atgtagtcca gcacttcgga ggccaggcgg caggcctggc
     6361 gcatgccggc aatgccctgg gcgtccttga tggtgatgtt gctcatggcg taaattatcc
     6421 cattcgcatt ggcgccttgc aggccaatgg tcgcgcggcg ggcgcaagcg gggtgcggcc
     6481 cgttaaaatg gcgggctttc acgaagacgc gccgccgggc gcggcagccc cattcagcgg
     6541 ctgcccgcac ccatcggccc ttgccccttt cccccatcac tccaggccgc taccgtgacc
     6601 ttgcacatca cccagctgga gggcggcaat gccctcagct ccttccgcgc ccagcaactg
     6661 ctgccccagc tgcaggccat ccaccccaag atcgaaggcc tggcggcgcg ccatgtgcat
     6721 ctggtggcgc gggatgccgc accgtccgag gccgagcgcg agcgcctggc cgcgctcttg
     6781 aactacggcg acccctacgc cggcccggcc gacggcctgg cgctgctggt cacgccgcgc
     6841 ctgggcacgg tctcgccctg ggcctccaag gcgaccgaca tcgcgcacaa ctgcggcctg
     6901 cagctgcgcc gcgtggagcg catcaccgaa taccgcatca gcctcaaggg cgggctgctg
     6961 ggcggcaggc ccgagctgac gccagagcag ctgcagcagg tcgccgcgct gctacacgac
     7021 cgcatgaccg agtcggtcgt cgccacgcgc gccgaggcac aagagctgtt caccgagctc
     7081 gacgccgagc ccatggcctt cgtggacgtg ctgggcggag gccgcgccgc cctggaagcc
     7141 gccaacaagg cctggggcct ggcgctggcc gatgacgaga tcgactatct ggtcaatgcc
     7201 ttcaagggcc ttgcgcgcaa ccccactgac gtggagctga tgatgttcgc ccaggccaac
     7261 agcgagcatt gccggcacaa gatcttcaac gcccagttca ccatcgacgg cgtggcacag
     7321 gacaaatcgc tgttcggcat gatccgccac accgaggccg tctcgcccca gcacaccatc
     7381 gtcgcctatg ccgacaacgc ctccatcatg gagggcagcg ccgtcgagca gttctttgcc
     7441 aaattcgaag ctttcgcagg ccaggcaagc gctaccagct atcaaaagag aagtggcacc
     7501 caccatgtgc tgatgaaggt ggagacgcac aaccacccca cggccatctc gcccttcccc
     7561 ggcgctgcga ccggcgcggg cggcgagatc cgcgacgagg gcgccaccgg ccgcggctcc
     7621 gagcccaagg ccggcctgac gggcttcacc gtgtccaagc tctggggcag ccaggttggc
     7681 aagcccgagc acatcgcgag tcccctgcag atcatgaccg agggcccgct gggcggcgcg
     7741 gccttcaaca acgagtttgg ccggccgaat ctgtgcggct acttccgcga atacgagcag
     7801 caggtggggg gcgtcacgcg cggctaccac aagccgatca tgatcgccgg cggcctgggc
     7861 accatcgatg cccagcacac gaagaagatc ctgttccccg ccggcacgct gttgatacag
     7921 ctcggcggcc cgggcatgcg catcggcatg ggtggcggcg cggccagctc catggccacg
     7981 ggcacgaatg cggctgagct ggactttgac tcggtgcagc gcggcaaccc cgagatcgag
     8041 cgccgcgcac aggaggtcat caaccactgc tgggcgcaag gggcagacaa ccccatcctg
     8101 gcgatccacg acgtgggcgc ggggggcctg tccaacgcct tccccgagct gaccaacgac
     8161 gccggccgcg gcgcgcgctt cgacctgcgt gccgtgccgc tcgaagaatc gggcctggcg
     8221 cccaaggaga tctggtccaa cgagagccag gagcgctacg tgctggccat cgcccccgag
     8281 tcgctggcgc agttcacagc gttctgcgag cgcgagcgct gccccttcgc cgtcgtcggc
     8341 gtcgccacgg ctgagcgtca gctgctcgtc ggtgacatcg acctgcacca cgccgaacgc
     8401 tccacgcccc acgcacaact tgccgtggac atgcccatgg acgtgctctt gggcaagccg
     8461 cccaagatgc accgcgacgt gcaaaccgtg cagcgcagct ttgcacccat tcagctcgac
     8521 ggcctggcgc tggagaaggc cgtcatcgac gtgctggccc accccacggt ggcctccaag
     8581 cgcttcctca tcaacattgg cgaccgcgcc gtgggcggcc tgacgcaccg cgaccagatg
     8641 gttggcccct ggcaggtgcc cgtggccgac gtggccgtaa ccctggccga ttacaagggc
     8701 tttgccggcg aggccatggc catgggcgag cgcacgccgc tggccgtgat caacgccccg
     8761 gcctcgggcc gcatggccgt cgccgaggcc atcaccaacc tgctggccgc gcccatcgaa
     8821 ctgccgcgcg tgaagctgtc ggccaactgg atggcggcct gcggcgaacc gggcgaggac
     8881 gccgacctgt acgccaccgt caaggccgtg ggcatggagc tgtgcccgca gcttggcatc
     8941 tcgattcccg tgggcaagga cagcctgtcc atgcgcacgc agtggcagga gggcgggcag
     9001 acgaagaagg tcacctcgcc cgtcagcctg atcatcaccg ccttcgccac gctggcggac
     9061 gtgcgcggca cgctcacgcc gcagctcgac gccggcgaag aagacagcac cctggtgctg
     9121 atcgacctgg gccgcggcaa aaaccgcatg ggcggctcca tcctgggcca ggtgctgggc
     9181 cagtcgggca atgaaacgcc ggacttggac gacgccaagg acctgatcgc cctggtgaac
     9241 gccgtgaacg cgctgcgggc tgaaggaaag atcctggcct accacgacaa gggcgacggc
     9301 ggcctgctgg ccacggtggc cgagatggcc tttgccggcc agctgggcgt ggcgctgaac
     9361 gtggacatgc tcatcaccga gggcgatggc atcagcgaca gccgcatgga cagcggcgag
     9421 ggcaagaact ggggccagca ggtcagcggc cggcgcgagg cccagacgct cgccgcgcta
     9481 ttcaacgagg agctgggcgt ggtgctgcag atccgcaccg ccgatcgcag cgccgtgatg
     9541 cagaccctgc gcgagcatgg cctgatccag tgcagccaca ccataggcaa gacgcgcccg
     9601 ctggcctcgc ccgtggacgc cggcaagggc gagctgcagg tctggcgcga cgccaagaag
     9661 gtgtttggcg ccagcctgtc ggacctgcac caggtctggg actccgtcag ctggaagatc
     9721 tgccagcagc gcgacaaccc ggcctgcgcc gacagcgaac atgccgccat gggcgatccg
     9781 tccaaccccg gcctgcatgt gcagctgacc ttcgacccgc tggacgacgt ggccgcgccc
     9841 tttgtgaacc tggcgcgccc caaggtggcc gtgctgcgcg agcagggcgt gaactcgcat
     9901 gtggagatgg cctacgcctt caccgaggcc ggcttcgagg ccttcgacgt gcacatgacc
     9961 gacctgcagg ccggccgcgc acgcctctca gactttgccg gcgtggtcgc ctgcgggggg
    10021 tttagctacg gcgacacgct gggcgcgggc attggctggg cgcgttccat caccttcaac
    10081 ccggccttgt ccgagcagtt ccagcaattc tttggccgtg gcgatacctt tgccctgggc
    10141 gtgtgcaacg gctgccagat gtttgccgag ctggccgaca tcatccccgg cgcccaggcc
    10201 tggccgcgct ttaccaccaa ccagagccac cgcttcgagg cgcgcctgtc catggtcgag
    10261 gtgctggcat cccccagcct gttcctgcag ggcatggccg gcagccgcct gcccatcgtc
    10321 gtctcgcacg gcgagggcta tgccaacttc gagcgccgcg gcaatgccga caaggtggtg
    10381 cccgccatgc gcttcgtcaa caaccatggc caggcgaccg aacagtaccc gttcaacccc
    10441 aacggcagcc cgggtggcct gaccgccgtg accacgcagg acggccgctt cacggccatg
    10501 atgccgcacc ccgagcgggc gtttcgcaac atccagatga gctggacgga cctggccgcc
    10561 accggtgggc aggatacgct gagcccctgg atgcgcatct ggcgcaatgc gcgcaagtgg
    10621 gtggggtaaa ccaattacta ttaaaacaat agcccctagc gcttgataca caagcgccag
    10681 gggctttttt cttcataatt ctgtttttta ttctgtggag tgatgcatgg ccggtgccag
    10741 ccttttgacc ctgctcgacg acatagccgc catcctggac gatgtggccc tgatgtccca
    10801 gatggccgcc aagaagtcgg ctgccatggc cgacgatgtc gcggtcatga ccaaggtggc
    10861 ggggcagaag accgctggcg tgctgggcga cgacctggcg ctcaacgccc agcaggtcac
    10921 cggcgtgcgc gccgagcgcg agatcccggt ggtctgggcc gtggccatag gctcgctggt
    10981 gaacaaggcc attttggtgc cggcggcgct ggccatcagc gcctgggcgc cctggctggt
    11041 cacgccgcta ctgatgctgg gcggggcctt tctgtgcttt gaaggggtgg agaagctggc
    11101 ccacaagtat ctgcacagcg ccgccgcaga cgccgctgcc catgccagcc atgcccaggc
    11161 caacgccgac cccgccatag acctggtggc catggagcgg ggcaagatca agggcgcggt
    11221 gcgcaccgac ttcatcctct cggccgagat catcgtgatt gcgctgggca cggtggccgc
    11281 ggcgccgttt gcacagcagg tggccgtgct ggtgagcatt gcgctgttga tgaccgtggg
    11341 cgtctatggc ctggtggccg gcatcgtcaa gcttgacgac ctgggcctgt ggctgaccgg
    11401 caaggccagc gcgctggcgc gggccctggg ccgggctctg ctggcggccg cgccccggct
    11461 gatgaagttt ctgtcggtgg cgggcatggc cgcgatgttt ctggtgggcg gaggcattct
    11521 ggtgcatggc gtgccggcgc tgcaccacgc catcgacggc ctgggcctgg ccgccgcagc
    11581 ctggcccctg ggcgggctgt ggtcgctgtt gactaccaac ctgctcaatg ccgtggtggg
    11641 cctggcggcg ggtgccgcag tgctgtcggc ctggctgctg ctggggcgcc tgcggggttc
    11701 tgccgtggcg cattgacggc actgcaggag agagaaagaa atttgccgaa ttacgggcaa
    11761 atgcctatgg acttttaaat attgacttaa agcaaagttg accattttgc tagtaggcct
    11821 aatgcgtgca cgggggtcgt ccccgtgcaa ccaaggtttc gagtccaatg aaaaagaatc
    11881 tcctggcact ggccatcctc tgtgcaacga ccgcgggcgc cgctcatgcg cagcaggacg
    11941 gcccatggct ggtgcgcgcc cgcgccgtgc atctggccag cgacaacggc ggcgccgcag
    12001 gcgcggcggg cgtgagcatc aacgacaaat ggatccccga ggtcgatgtg tcgtatttct
    12061 ttacacccaa ctttgccgcc gagctgatcc tgacctaccc gcaaaagcac gacgtgcgcc
    12121 tgaacggcgg caagatcggc agcgtcaagc atctgccgcc cacgctgctc gcccagtacc
    12181 acttcaccgg cctgggcgcg ttcaagccct atgtgggcgc gggcatcaac tacacgcgct
    12241 tctcgggtgt ggacattgcc ggtggcgcgc tgacggtgaa gaagaacagc tggggcccgg
    12301 ccctgcaact gggctttgac tacgcgctgg acaagcactg gtcgctgaac ctcgacgtga
    12361 agaaggtcta tatagacacc acggtcagtg gcggcgtggg ccggttcaag gtcgatccgg
    12421 tgctggtggg gctgggtgtg ggctaccgct tctga
//
LOCUS       DAOMTQ010000088        12338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_916_length_12338_cov_23.461776, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000088 DAOMTQ010000000
VERSION     DAOMTQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12338
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_12338_cov_23.461776"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PK518_09645"
     CDS             <1..289
                     /locus_tag="PK518_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRM49219.1"
                     /translation="WRLDGLHALELHDAFAAQGLAFCDALGLPHAAINRAGGGLARGH
                     PIGASGAIALVRVLGELWRQMRAQGGAGALRGMACIAGAGGLGTATWVEME"
     gene            295..993
                     /locus_tag="PK518_09650"
     CDS             295..993
                     /locus_tag="PK518_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRM49220.1"
                     /translation="MTEPLALAPLEDDYEALARARHSVRAFLPRPVPEALLERLLTTA
                     RLAPSGANLQPGRFWAVQGAARERLVAALCQAWRDHVPEAEDYGYFPRPMPLQLRKRQ
                     VAAAQALYGALGVARGDGEGRALQFERNFRFFDAPVALVVTIERDFGAGGYMDLGMCL
                     HGLMLAAQAQGLASCAIGALASYPQIVRAALGLPESEAVVCGLALGWEDAQAPVNRTR
                     TEREPLARYFSVLR"
     gene            1127..2467
                     /locus_tag="PK518_09655"
     CDS             1127..2467
                     /locus_tag="PK518_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM49221.1"
                     /translation="MSLHQQLLQRARQGRPIRIGLIGAGKFGAMYLAQVPRMPGVHLV
                     AIADLSPDNARASLARVGWPMEQSAAASGQQALAEGTTWITDDWQAVTRDPHIELIVE
                     CTGNPLAAVDHILDAFAHGKHVVNVTVEADAFCGPLLAARAAQAGVIYSLAYGDQPAM
                     ICDLVDWARTCGFPVVAAGRGHKWLPHFCESTPETVWGYYGLTPEQAVRGGLNPKMFN
                     SFLDGSKPAIESTAVANATGLAVPSNGLLYPPASVEDIPFVTRPLAEGGVLEQKGMVE
                     VISSLEKDGRKIPYDIRMGVWVTVEGESDYIRHCFEEYNAHTDPSGRYFTLYKRWHLI
                     GLEVGMSVASVALRGEATGVAQGWNADVVATAKRDLRVGELLDGEGGYTVWGKLLPAA
                     TSRALGGLPLGLAHGVKLVRPVAKGQSLCWDDVAMDTSSAAYRLRREMEALSAG"
     gene            2496..>3704
                     /locus_tag="PK518_09660"
     CDS             2496..>3704
                     /locus_tag="PK518_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRM49222.1"
                     /translation="MPLQISFPESLPVSARRDEIMAAMAEHQVIIVCGETGSGKTTQL
                     PKIALALGRGRCNAPEGERGRLIGHTQPRRIAASSVAKRIAEELKTPLGEVVGYKVRF
                     ADTLQKGASVKLMTDGILLAETQTDPLLKAYDTLIIDEAHERSLNIDFLLGYLKQILP
                     QRPDLKVVVTSATIDAERFAKHFASKAGPAPVIMVSGRTYPVEMRWRPFEEKKDFDLN
                     DAIADGVDELWAGGAGGDILIFLPGEREIREAADHLRKHLQHSPVLRSAEVLPLFSRL
                     SQAEQDRIFDSHSHRRIVLATNVAETSLTVPGIKYVVDAGTARVKRYSFRSKVEQLLV
                     EPISQAAANQRAGRCGRVANGICIRLYDEADFNQRDRFTDPEILRSSLAGVILRMKSL
                     GLGDVVNFPFL"
     assembly_gap    3705..3804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3805..6250
                     /locus_tag="PK518_09665"
     CDS             <3805..6250
                     /locus_tag="PK518_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HRM49223.1"
                     /translation="QPSPQPSPRGRGSENHGAGSSLPLPPAGEGRGEGASDTHKLSNR
                     QWEQLLRQNYLSIRRVREWRDIHSQLLTVVREHQWQLNAQPAGYEAVHLSMLAGLLGN
                     IGFKGEESEAYLGAHGIKFHPHPGAHLSKKPGRWIVAAEQVETSRLYGRGIAAIEPQW
                     LEEVGGHLLRKQLLDPHWSKKQADVVAYERATLYGLVVYNQRGVSYGRIDPIEARQMF
                     IRRALVEGEWDSPWPFLAANHKTVRKVEELEHKSRRQDVLVDDELIYAFYDQAIPEGI
                     YSGATFDKWFRAVSKGQPELLRLSKDELMRHEAAGITTDKFPKTVKLGGVDCSASYLH
                     SPGDARDGLTVTVPLFVLNQVSEERAEWLVPGMLKDKIQALLKSLPQRPRSRFVPLPE
                     HAERLAQLFLQGERWQQGGLIDVLLKQARDETSLDIKRADFKLDMLSAHLFMNLRVTD
                     EHGRQLGQSRNLGALKAEWGGKARGAFQALAELKLAATPAAAASLPSPPGRGQGEGKA
                     GAAPVASSPHPSPLPKGEGAKTQAEARYTTWSFGELPELMELKKGGHTLIGFPALIDQ
                     GDAVTIEVFDEPELAAAKHHLGLRRLCALQIKDALKYLEKNIPDLQKMAVAYMPLGTQ
                     EELRAQIIDVAMDRAFLQEPLPTDAAQFAQRVQEGRGRLTLIANEVARMAAVILAEYA
                     SAQRKIKDTKNAPQATTDTGNQLQKLMPKGFVAAAPWVQLQHYARYLKAITTRLDKLR
                     ADPARDAAKLQELQPLEQRYWRLVAERKGQQDARMQEYRWMLEELRVSFFAQELRTPY
                     PVSTKRLDKLWAQLQR"
     gene            complement(6308..6853)
                     /locus_tag="PK518_09670"
     CDS             complement(6308..6853)
                     /locus_tag="PK518_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM49224.1"
                     /translation="MKNYLLALVLGLAALGAQAVEMEISAKDVYEKVQGNDPGVLFVD
                     VRDPLEIMFTGFTDVVHVNIPYLLADRTRWEDERSIYRLYRNPDFVAQIKAELARRGM
                     KEDAEVITLCRSGSERGKPSAQFLRDNGLPNARYVVNGFQGSPVKDGPQAGLRIVNGW
                     QNSGLPWSAKMSPGKMFRVDK"
     gene            complement(6946..7281)
                     /locus_tag="PK518_09675"
     CDS             complement(6946..7281)
                     /locus_tag="PK518_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49225.1"
                     /translation="MVHMGFALRALSSISSLCNPLLRSHSWHKPAPDSQPQLHAAPPV
                     HVSRTAPGADRCFGVAPQAPAPATPRPTASTRPLRVILRQQDDGACRLLITGRMADVC
                     AELDRLALH"
     gene            complement(7427..8101)
                     /gene="lexA"
                     /locus_tag="PK518_09680"
     CDS             complement(7427..8101)
                     /gene="lexA"
                     /locus_tag="PK518_09680"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRM49226.1"
                     /translation="MLDSPKLTARQQQILDLIQTAIARTGAPPTRAEIAAELGFKSAN
                     AAEEHLQALARKGVIELVSGTSRGIRLCGETVRHINAVRGTQFSLPIPGLSQLTLPLI
                     GRVAAGSPILAQEHVDQTYTVEGNLFAHKPDYLLKVRGMSMRDAGIMDGDLLAVQATR
                     EARSGQIIVARLGDDVTVKRLRRTGRAIELLPENPDYPVIRVEPGEPFEIEGLAVGLI
                     RNTMLM"
     gene            8193..9143
                     /locus_tag="PK518_09685"
     CDS             8193..9143
                     /locus_tag="PK518_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRM49227.1"
                     /translation="MERKVVVLGTGGTIAGTSDMAGANVGYTAGQIGVEQLLSAIPAL
                     ARAAGASLVAEQIAQIDSKDMGFEVWARLAQRCALHLADPLVAGIVITHGTDTLEETA
                     WLLANLLDATRPVVLTCAMRPATALAPDGPQNLLDAVALATEPTAHGVLMVAAGVVHG
                     AREVAKVHPLRLDAFDSGDTGPLGWVEDGRLRWAHGRAPQPAAPRHAGLLPALGRSPW
                     PRVEIILSHAGSDGALVDWLVQGGARGIVVAATGNGTLHQALERALERAVAAGVQVRV
                     ASRCPQGRMLDLHDAPWQDAGGLSPVKARVSLLLELMQGA"
     gene            complement(9199..9855)
                     /gene="adk"
                     /locus_tag="PK518_09690"
     CDS             complement(9199..9855)
                     /gene="adk"
                     /locus_tag="PK518_09690"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRM49228.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQKFGIPQISTGDMLRAAVKAGTP
                     LGLQAKAVMDAGQLVSDELIINLVKERLTQPDCANGFLFDGFPRTIPQADAMKAAGVK
                     LDYVLEIDVPFDAIIERMSGRRSHPASGRTYHVKFNPPKVAGQDDVTGEPLVQREDDK
                     EETVKKRLQVYSDQTRPLVDYYSHWAKAEPAAAPKYRAIQGTGSVEEITERALSALSS
                     "
     gene            complement(9967..10755)
                     /gene="kdsB"
                     /locus_tag="PK518_09695"
     CDS             complement(9967..10755)
                     /gene="kdsB"
                     /locus_tag="PK518_09695"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRM49229.1"
                     /translation="MSTAAAPFTVLIPARLASTRLPNKPLADIAGLPMVVHVARRAAQ
                     SAALRCVVAADDGRIVDACRLHGVEALLTRADHASGSDRLAEACEQLSLAGDDIVVNV
                     QGDEPLIDPQLIDAVAALLHQRADASMGTAAHAIASAADFANPNVVKVVLDAHGLAHY
                     FSRAPIPHARDHAAGSCWWQPGQGGPAGFAPLRHIGIYSYRAGFLRGFPQLPPAPTEQ
                     LEALEQLRALWHGHRIAVHVTASEPGAGVDTPTDLERVRALLAG"
     gene            complement(10752..10934)
                     /locus_tag="PK518_09700"
     CDS             complement(10752..10934)
                     /locus_tag="PK518_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRM49230.1"
                     /translation="MDPKLLELLVCPVTKGPLTYDREHQELISRSARLAYPVRDGIPV
                     LLENEARTLTDEELER"
     gene            complement(10943..11938)
                     /gene="lpxK"
                     /locus_tag="PK518_09705"
     CDS             complement(10943..11938)
                     /gene="lpxK"
                     /locus_tag="PK518_09705"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRM49231.1"
                     /translation="MSAAARLRQAWQQRGPLALLLWPLTLPYRLLTALRRALYRTGLL
                     KQHRLPVPVIVVGNVIVGGAGKTPVTLALLQHLRAAGWRPGVISRGYGRQTISCRPAL
                     PTSSAAEVGDEPLLIARSAGVPVYVARRRADAGRALLAAHPEVNILVCDDGLQHLQLA
                     RDVEVCVFNDEGIGNGWLLPAGPLREPWPRAVDLVLHAGAAPGGSAPQFPLSRRLADH
                     AVDAKGRQLPLAQLRGLKLHALAAIARPQDFFAMLRAQGLPPATEEALPDHYDFSSWK
                     RPSDKRLIPICTEKDAVKLWPAHPGALAVPLRLDIPAAFFAALDARLASLSSPRH"
     gene            complement(11943..>12338)
                     /locus_tag="PK518_09710"
     CDS             complement(11943..>12338)
                     /locus_tag="PK518_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRM49232.1"
                     /translation="PEINLIPFIDVLLVILIFLMLSTTYSKFTELQLTLPVADAEQLR
                     DHPKEVIVAVAADGRYAINKTPLEGKHVEQVARALSEAAVAGPKSVVIISADAAAPHQ
                     SVVTVMEAARRVGLSQITFATQSSAQAGR"
BASE COUNT         1950 a         3942 c         4377 g         1969 t
ORIGIN      
        1 ctggcggctg gacggcctgc acgcgctgga gctgcacgac gcctttgccg cccagggcct
       61 ggccttctgc gacgcgctgg gcctgccgca cgcggccatc aaccgcgctg gcggcggcct
      121 ggcgcgcggc caccccatag gcgcctcggg cgccatcgcc ctggtgcgcg tgctgggcga
      181 gctgtggcga cagatgcggg cacagggcgg cgccggggcg ctgcgtggca tggcctgcat
      241 agccggtgcg ggcggcctgg gcaccgccac ctgggtggag atggaatgat gcggatgacc
      301 gaacccctgg cgctggcgcc gcttgaagat gactacgagg cgctggcccg cgcacgccat
      361 tccgtgcgcg cctttctgcc gcgccccgtg ccagaggcgc tgctcgaacg cctgttgacc
      421 accgcgcgcc tggcgcccag cggcgcgaac ctgcagcccg ggcgcttctg ggccgtgcag
      481 ggcgcggcgc gcgagcgcct cgtcgccgcc ctgtgccagg cctggcgcga ccatgtccct
      541 gaggccgagg actacggcta cttcccgcgc cccatgccgc tgcagctgcg caagcgccag
      601 gtggcggcgg cgcaggcgct gtatggcgcc ctgggcgtgg cgcgcggcga tggcgagggg
      661 cgcgcgctgc agtttgagcg caacttccgc tttttcgacg cgcccgtggc cctggtcgtg
      721 accatagagc gcgactttgg cgccggcggc tacatggatc tgggcatgtg cctgcacggc
      781 ctgatgctgg cggcgcaggc ccagggcctg gcgagctgcg ccataggcgc gctggccagc
      841 tacccgcaga tcgtgcgcgc tgcgctgggc ctgcccgagt ccgaggccgt ggtctgcggc
      901 ctggccctgg gctgggagga cgcgcaggcg cccgtgaacc gcacacgcac cgagcgcgag
      961 cctttggcgc gctatttcag cgtgttgcgc tgaccgggtc aggtcttgtc cggagagacg
     1021 ccggcgcggc cctgggttac ggtggccccg gcttgaccca cgcaccggcc gcctgtctgc
     1081 gggcgccggc ttcgcccctc gcggcgctct ctctgccccc tgtttcatga gtcttcacca
     1141 gcaactgctt cagcgcgccc gccagggccg ccccatccgc atcggcctga taggggccgg
     1201 caaatttggc gccatgtacc tggcgcaggt gccgcgcatg cccggcgtgc atctggtggc
     1261 gattgccgat ctgtcacctg acaacgcgcg cgccagcctg gcgcgcgtgg gctggccgat
     1321 ggagcagtcc gccgccgcgt cgggccagca ggcgctggcc gaaggcacga cctggatcac
     1381 cgacgactgg caggccgtca cgcgcgaccc gcacatcgag ctcatcgtcg aatgcaccgg
     1441 caacccgctg gctgcagtgg atcacatcct ggacgccttt gcgcatggca agcatgtggt
     1501 caatgtgacc gtcgaggccg atgccttttg cggcccgctg ctggcggcgc gggcggcgca
     1561 ggcgggggtg atttactcgc tggcctatgg cgaccagccg gccatgattt gcgatctggt
     1621 cgactgggcg cgcacctgcg gctttcccgt ggtggcggcc gggcgcggcc acaaatggct
     1681 gccgcatttc tgcgaatcga cgcccgagac ggtctggggc tactacggct tgacccccga
     1741 gcaggcggtg cgcggcgggc tgaaccccaa gatgttcaac agctttctcg acggctccaa
     1801 gcccgccatc gagagcacgg cggtcgccaa cgccaccggg ctggccgtgc cgagcaatgg
     1861 ccttttgtac ccgccggcca gcgtggagga cattccgttc gtcacccgcc ccctggccga
     1921 gggcggcgtg ctggagcaaa agggcatggt cgaggtgatc tcgtcgctgg agaaagacgg
     1981 gcgcaagatt ccctacgaca tccgcatggg cgtgtgggtg acggtggagg gcgagagcga
     2041 ctacatccgc cattgcttcg aggaatacaa cgcccatacc gacccgagcg ggcgctattt
     2101 cacgctctac aagcgctggc acctcatcgg cctggaggtc ggcatgtcgg tcgccagcgt
     2161 cgcgctgcgc ggcgaggcca cgggcgtggc ccagggctgg aatgccgacg tggtggccac
     2221 cgccaagcgc gacctgcgcg tgggcgagtt gctcgacggc gagggcggct acaccgtctg
     2281 gggcaagctg ctgcctgccg cgacttcgcg cgcgctgggc ggcctgccgc tggggctggc
     2341 ccatggcgtg aagctggtgc ggccggtcgc caaggggcag tcgctgtgct gggacgacgt
     2401 ggccatggac accagcagcg cggcctaccg gctgcggcgc gagatggagg cgctgtcggc
     2461 aggctgaatc cggggctggg ataatccccg cccctatgcc tttgcagatc agctttcccg
     2521 aatcccttcc cgtctccgcg cgccgcgatg aaatcatggc cgccatggcc gagcaccagg
     2581 tcatcatcgt ctgcggtgag accggctcgg gcaagaccac gcagctgcca aagatcgcac
     2641 tggccttggg acgcgggcgc tgcaatgcac cagagggcga gcgtggccgg ctgattggcc
     2701 atacccagcc gcggcgcata gcggccagca gcgtcgccaa gcgcatcgcc gaagagctga
     2761 agaccccgct gggcgaggtg gtgggctaca aggtgcgctt tgccgacacg ctgcaaaagg
     2821 gcgcctctgt caagctgatg acggacggca tcttgctggc cgagacgcag accgatccgc
     2881 tgttaaaggc ctacgacacc ctcatcatcg acgaggcgca tgagcgcagc ctgaacatcg
     2941 attttctgct gggctacctc aagcaaatcc tgccgcagcg gcccgatctg aaggtggtgg
     3001 tgacatcggc caccatagac gccgagcgct ttgccaagca ctttgcaagc aaggccggcc
     3061 cggcgccggt catcatggtg tccgggcgca cctatccggt ggagatgcgc tggcggccgt
     3121 tcgaggaaaa gaaggacttc gacctcaacg atgccattgc cgacggcgtc gatgagctct
     3181 gggctggggg cgcgggtggc gacatcctga tcttcctgcc cggtgagcgc gaaatccgcg
     3241 aggcggccga ccacctgaga aagcatttgc agcattcgcc ggtgctgcgt agtgccgagg
     3301 tgctgccact gttttcgcgc ctgtcgcagg ccgagcagga tcggattttt gacagccaca
     3361 gccaccgccg catcgtgctg gcgaccaacg tggccgagac ttcgctcacg gtgccaggca
     3421 tcaaatacgt tgttgacgca ggtacggcgc gcgtcaaacg ctattctttt agaagcaaag
     3481 tggagcagct gctggtcgag cccatcagcc aggcagccgc caaccagcgc gccgggcgct
     3541 gcgggcgcgt ggccaacggc atctgcatcc gcctgtacga cgaggcggat ttcaaccagc
     3601 gcgaccgctt taccgacccc gaaatcctgc gctcatcgct cgccggcgtc atcctgcgca
     3661 tgaagagcct gggcctgggc gacgtggtga actttccgtt tctgnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnngcagcc ctcaccccag ccctctccca gagggagagg
     3841 gagtgaaaac cacggcgctg ggagttctct ccctctcccg cctgcgggag agggcagggg
     3901 tgagggtgca tccgacaccc acaaactcag caaccgccag tgggagcagc tcttacgcca
     3961 gaactacctg agcatccgcc gcgtgcgcga gtggcgcgac atccattcgc agctgctgac
     4021 cgtggtgcgt gagcaccagt ggcagctcaa cgcccagccg gcgggctacg aggcggtgca
     4081 cctgtccatg ctcgccggcc tgctcggcaa catcggtttc aagggcgagg agagcgaggc
     4141 ctacctgggc gcgcatggca tcaagttcca cccgcatccg ggcgcgcatt tgtcgaagaa
     4201 gcccgggcgc tggatcgtcg ccgccgagca ggtggagacc tcgcgcctgt atggccgggg
     4261 cattgccgcc atcgagccgc agtggctgga agaggtgggc ggccatttgc tgaggaagca
     4321 gctgctcgac ccgcattgga gcaagaagca ggccgacgtg gtggcctacg agcgcgccac
     4381 gctctatggc ctggtggtct ataaccagcg cggcgtgagt tatggccgca tcgacccgat
     4441 cgaggcgcgg cagatgttca tccgccgcgc gctggtcgag ggcgagtggg acagcccctg
     4501 gcccttcctg gccgcgaatc acaagacggt gcgcaaggtc gaagagctgg agcacaagag
     4561 ccggcgccag gacgtgctgg tggacgacga gttgatctac gccttctacg accaggccat
     4621 tcccgagggc atttacagcg gcgccacctt tgacaagtgg tttcgcgccg tcagcaaggg
     4681 ccagcccgag ctgctgcgcc tctctaaaga cgagctcatg cgccacgagg ccgcgggcat
     4741 caccaccgat aaattcccca agaccgtcaa gctcggcggc gtggactgct ctgccagcta
     4801 cctgcacagc ccgggcgacg cgcgcgatgg tctcaccgtg accgtgccgc tgttcgtgct
     4861 caatcaggtg agcgaggaac gcgccgaatg gctggtgccg ggcatgctca aggacaagat
     4921 ccaggcgctc ctgaaaagcc tgccacagcg cccgcgcagc cgctttgtgc cgctgcctga
     4981 acacgccgag cgcctggcgc agctgttctt gcagggcgag cgctggcaac agggcgggtt
     5041 gatcgacgtg ctgctcaagc aggcgcgcga tgaaacctcg ctcgacatca agcgcgccga
     5101 cttcaagctc gacatgctca gcgcgcacct gttcatgaac ctgcgcgtga ccgacgagca
     5161 tggccgccag ctggggcaaa gccgcaacct gggcgcgctg aaagccgaat ggggcggcaa
     5221 ggcgcgtggg gcgttccagg cgctggcgga gttgaagctt gcggccaccc cggcggcggc
     5281 cgcgtcttta ccctctcccc ctgggagagg gcagggtgag ggcaaggccg gcgcagcgcc
     5341 tgttgccagc agccctcacc ccagccctct cccaaaggga gagggagcga aaacccaggc
     5401 agaggcgcgc tatacaacct ggagctttgg cgagctgccc gagctgatgg agctgaaaaa
     5461 aggcggccat acgctgatcg gcttcccggc gctgatcgac cagggcgacg ccgtgaccat
     5521 cgaggtcttt gacgagcccg aactggctgc ggccaaacac cacctgggcc tgcgccgcct
     5581 gtgcgcgcta cagatcaagg acgcgctgaa atacctggaa aagaacattc cggatctgca
     5641 aaaaatggcc gtggcttaca tgccgctggg cacgcaggag gagctgcgtg cgcaaatcat
     5701 cgacgtggcc atggatcgcg cctttctgca ggagcccctg cccacggacg cggcccaatt
     5761 tgcccagcgc gtgcaggaag gccgtggccg cctgaccctg atcgccaatg aagtggcgcg
     5821 catggcggcg gtgattctgg ccgaatacgc cagcgctcaa cgcaagatca aggatacaaa
     5881 aaatgcgcca caggccacga ctgacacggg caaccagcta caaaaactga tgcccaaggg
     5941 gtttgtggcc gccgcgccct gggtgcagct gcagcattac gcgcgctacc tgaaagccat
     6001 caccacgcgt ctggacaagc tgcgtgctga cccggcgcgt gacgccgcca agctacagga
     6061 gctgcagccc ctggagcagc gctactggcg cctggtcgca gagcgcaagg gccagcagga
     6121 cgcgcgcatg caggaatacc gctggatgct cgaagagctg cgcgtgagtt tctttgccca
     6181 ggaactgcgc acgccgtacc cggtgagtac caagcgcctg gacaagctgt gggcgcagtt
     6241 gcagcgatga actatcaaaa atgatagctt ttagcgcttt attggtgcgc gccatcgcca
     6301 aaaaccgcta cttatccacg cggaacatct tgcccgggct catcttggcg ctccagggca
     6361 ggccgctgtt ttgccagccg ttgacgatgc gcagcccggc ctgggggccg tccttgaccg
     6421 ggctgccctg gaagccgttg acgacatagc gtgcattggg caggccgtta tcgcgcagga
     6481 attgggcgct gggcttgcca cgctcgctgc ccgagcggca cagggtgatg acctcggcat
     6541 cctctttcat gccgcggcgc gccagctcgg ccttgatctg ggccacaaaa tcggggttgc
     6601 ggtagaggcg gtagatgctg cgctcgtcct cccaacgggt gcgatcggcc agcagatagg
     6661 gaatgttcac atgcaccacg tcggtgaagc cggtgaacat gatttccagc gggtcgcgca
     6721 catcgacaaa aagtacgccg gggtcgttgc cctgcacctt ttcgtagaca tccttggcgg
     6781 aaatttccat ctccaccgcc tgcgcgccca gggctgccag gcccagaacc agggccagta
     6841 ggtagttttt cattgcagta ctccgtcttg aggaaagaga gatgcagtgt attagcaatt
     6901 acttaaattg catccgtgcc gccggttgcg cgcccgtggc ggacatcagt gcagcgccag
     6961 gcgatcgagt tcggcgcaga catccgccat acgcccggtg atcagcaggc ggcaggcgcc
     7021 gtcgtcctgc tggcgcagga tcacacgcag cgggcgggtg gacgccgtgg ggcgtggcgt
     7081 agcgggtgct ggcgcttgcg gtgcgacccc gaagcagcgg tcggcgcctg gggcagtgcg
     7141 gctgacatgc acgggtggtg cagcgtgcaa ctgcggctgg ctgtctggcg cgggcttgtg
     7201 ccatgaatgg ctgcgcagca gcgggttgca caggctggaa atggacgaca gcgcgcgcag
     7261 ggcgaatccc atgtgaacca tgtgaagact ccttgatgcc aatggcatga aagtgaaaca
     7321 caggcaggat tgccaagaaa aagacgcgca cctgcagggt tggctgcagg ccaacagcat
     7381 ggcccgcagt ccaaacgccc gccacctgcg gcggcgccgg ccatgcctac atcagcatgg
     7441 tgttgcggat caggcccacg gccaggccct caatctcgaa gggctcgccg ggctcgacgc
     7501 ggatgacggg atagtcgggg ttctcgggca gcagctcgat ggcgcggccg gtgcgacgca
     7561 ggcgcttgac ggtgacgtcg tcacccaggc gcgcgacgat gatctggccg ctgcgcgcct
     7621 cgcgtgtggc ctgcacggcc agcaggtcgc cgtccatgat gccggcgtcg cgcatggaca
     7681 tgccgcgcac cttgagcagg tagtcgggct tgtgcgcgaa caggttgccc tccaccgtat
     7741 aggtttgatc cacatgctcc tgcgccagaa tgggcgaacc cgccgcgacg cggcctatga
     7801 gcggcaaggt gagctgggac aggccgggaa tcggcaggct gaactgggtg ccgcgcacgg
     7861 cgttgatgtg gcgcacggtc tcgccgcaca gccgtatgcc gcgcgaggtg ccgctgacca
     7921 gctcgatcac acccttgcgc gccagggcct gcaggtgctc ctcggccgcg ttggcggact
     7981 tgaaaccgag ctcggccgcg atttcggcac gcgtgggcgg cgcgccggtg cgcgcgatgg
     8041 cggtctggat caagtccaga atctgctgct ggcgggcggt gagcttgggg ctgtcgagca
     8101 tgtggtgagt ccggtgctgg cggttggagg gttactgttg tttaatccag tggctgtatt
     8161 tttgaacagt ttttgaaaag gcgcaagcgg gtatggaaag aaaagtcgtg gtgctgggca
     8221 ccgggggcac gattgccggt acgtccgata tggctggggc caatgtgggc tacacggccg
     8281 ggcagatcgg ggtggagcag ttgctgtccg ccattcccgc ccttgcgcgc gcggccggcg
     8341 ccagcctggt ggccgagcaa attgcgcaga tcgacagcaa ggacatgggt tttgaggtct
     8401 gggcgcggct ggcgcagcgc tgcgcgctgc acctggccga tcccttggtg gccggcatcg
     8461 tcatcaccca tggcacggac acgctggaag agacggcctg gctgctggcg aacctgctgg
     8521 atgccaccag gcccgtggtg ctgacctgcg ccatgcgccc ggccacggcg ctggcgccgg
     8581 acgggccgca gaacctgctg gatgccgtgg ccctggcgac cgagcccacg gcgcatggcg
     8641 tgctgatggt ggccgccggt gtggtgcatg gcgcgcgcga ggtggccaag gtgcacccgc
     8701 tgcggctgga tgcgttcgac tccggcgaca ccgggcccct gggctgggtg gaggatggcc
     8761 gcttgcgctg ggcccatggc cgggcgccgc agccggccgc gccccggcat gccggactgc
     8821 tgcccgcgct gggccgcagc ccctggccgc gcgtggaaat cattctcagc catgcgggca
     8881 gcgatggtgc gctggtcgat tggctggtgc agggcggcgc ccggggcatc gtggttgcgg
     8941 cgactggcaa cggcaccttg caccaggcgc tggagcgggc cctggagcgt gccgtggccg
     9001 cgggtgttca ggtgcgcgtg gcctcgcgct gcccgcaggg gcgcatgctg gatctgcacg
     9061 atgcgccgtg gcaggacgct gggggcctga gccccgtgaa ggcgcgcgtg agtctgctgc
     9121 tggagttgat gcagggggcg tgaggcccga aaagcacaac gccccgcgtt gcggggcgtt
     9181 ggtggtgccg aggcaggatc agctgctcag ggccgacagt gcgcgctcgg tgatctcttc
     9241 cacgctgccc gtgccctgga tggcgcggta cttgggcgcg gcggccggct cggccttggc
     9301 ccagtgggag tagtagtcga ccagagggcg cgtctggtcg ctgtagacct gcaggcgctt
     9361 cttgacggtt tcttccttgt cgtcctcgcg ctgcaccagg ggctcgccgg tcacgtcgtc
     9421 ctggccggcc accttgggcg ggttgaactt gacatggtag gtacggccgc tggccgggtg
     9481 gctgcggcgg ccgctcatgc gttcgatgat ggcgtcgaac ggcacgtcga tttccagcac
     9541 gtagtccagc ttcacgccgg cggccttcat ggcgtccgcc tggggaatgg tgcgcgggaa
     9601 gccgtcgaac agaaagccat tcgcgcagtc gggctgcgtc aggcgttcct tgaccaggtt
     9661 gatgatcagc tcgtcgctga ccagctggcc ggcgtccatg acggccttgg cctgcaggcc
     9721 caggggcgtg ccggccttga cggcggcgcg cagcatgtca ccggtggaga tttgcggaat
     9781 accgaacttc tggcagatga aggcggcttg cgtgcccttt ccggcgccgg gggcgcccag
     9841 caatatcagt ctcatggata tcctctggag ttttgaaatc gtgcggcacg ctggccggta
     9901 ggtggctggt gctcttgtcg cggcgaggat agcacgcgag tcctgcgcca agctgacggc
     9961 cggcgcctac cccgccagca gggcgcgcac gcgttccagg tcggtggggg tgtccacgcc
    10021 ggcgcccggc tcgctggccg tcacatgcac ggcgatgcgg tggccgtgcc agagcgcgcg
    10081 cagttgctcc agcgcctcca gctgctcggt gggtgcgggt ggcagctgcg gaaagccgcg
    10141 cagaaaaccg gcgcggtagc tgtagatgcc gatgtggcgc agcggcgcaa agccggccgg
    10201 gccgccctgg ccgggctgcc accagcagct cccggcggcg tggtcgcgcg catgggggat
    10261 cggcgcacgg ctgaagtagt gcgccaggcc atgtgcgtcc agcaccacct tgaccacgtt
    10321 ggggttggca aagtcggcgg cgctggcgat ggcatgggcg gccgtgccca tgctggcgtc
    10381 ggcgcgctgg tgcagcagcg cggccacggc gtcgatgagc tgcgggtcga tcagtggctc
    10441 gtcgccctgc acgttgacga cgatgtcgtc tccggccagg ctcagttgct cgcaggcttc
    10501 ggccaggcgg tcgctgccac tggcgtggtc ggcgcgggtc agcagggcct cgacgccgtg
    10561 caggcggcaa gcatcgacga tgcgaccatc gtcggccgcc accacgcaac gcagggcggc
    10621 gctttgcgcg gcgcggcgcg ccacatgcac gaccatgggc aggccggcga tgtcggccag
    10681 cggcttgttc ggcaggcgcg tggaggccag gcgcgccggg atcagcaccg tgaagggcgc
    10741 ggcggcggtg ctcaccgctc cagctcctcg tcggtgagcg tgcgggcctc gttctccagc
    10801 agcacgggaa tgccgtcgcg cacggggtag gccaggcgcg cgctgcgcga aatcagctcc
    10861 tggtgttctc ggtcataggt cagcgggccc ttggtgacgg ggcagaccag cagttcaagc
    10921 agtttcgggt ccatggtgtt cctcaatggc ggggcgatga tagcgaggcc aggcgtgcgt
    10981 ccagcgcggc gaagaaggcc gccggtatat cgaggcgcag gggcacggcc agggcgccgg
    11041 ggtgggcggg ccagagcttg acggcgtctt tttcggtaca aatcggtatc aagcgcttat
    11101 ctgacgggcg cttccagcta gaaaaatcat agtgatctgg cagcgcctct tcggtcgcgg
    11161 gcggcaggcc ctgggcccgc agcatggcaa aaaagtcttg cgggcgggca atggcggcca
    11221 gggcgtgcag tttcaggccg cgcagctgcg ccagcggcag ttggcggccc ttggcgtcca
    11281 cggcatggtc ggcgaggcgg cgcgatagcg ggaactgcgg cgccgatccg ccgggcgccg
    11341 cgccggcgtg cagcaccaga tcgacggcgc gcggccaggg ttcacgcagc gggccggcgg
    11401 gcagcagcca gccgttgcca atgccctcgt cgttgaagac gcagacctcc acgtcgcgtg
    11461 ccagttgcag gtgctgcagg ccgtcgtcgc agacgaggat gttgacctcg gggtgggccg
    11521 ccagcagcgc ccgaccggca tcggcgcggc ggcgcgccac gtacaccggc acgccggcgc
    11581 tgcgggcgat cagcagcggc tcgtctccca cctcggcggc gctgctcgtc ggcagggccg
    11641 ggcggcagct gatggtttga cggccatagc cgcgcgagat gacgcccggg cgccagcccg
    11701 cggcacgcag atgttgcagc agggccaggg tgacgggggt cttgccggcg ccgccgacga
    11761 tgacgttgcc gaccacgatg accggcaccg gcaggcggtg ctgtttcagc aggcccgtgc
    11821 ggtagagcgc gcggcgcagc gccgtcagca gccggtaggg cagggtcagt ggccacagca
    11881 gcagcgccag cgggccgcgc tgctgccagg cctggcgtag ccgcgcggcg gcgctcatgc
    11941 gatcagcggc cggcctgcgc cgacgattgg gtggcaaagg tgatctgcga caggcccacg
    12001 cggcgcgcgg cctccatcac cgtgaccacg gactggtgcg gcgcggcggc atcggcgctg
    12061 atgatgacca cgctcttcgg cccggcgacg gcggcctcgg acagcgcgcg cgccacctgc
    12121 tcgacatgct tgccttccag cggggtcttg ttgatggcat agcgtccgtc ggcggctacg
    12181 gccacgatga cctccttggg gtggtcgcgc agctgttcgg cgtcggccac gggcagggtc
    12241 agctgcagct cggtgaactt gctgtaggtg gtggacagca tcaaaaagat gaggatcacc
    12301 agcagcacgt cgatgaacgg gatcaggttg atctccgg
//
LOCUS       DAOMTQ010000089        12323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_919_length_12323_cov_29.136697, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000089 DAOMTQ010000000
VERSION     DAOMTQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_12323_cov_29.136697"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PK518_09715"
     CDS             complement(<1..993)
                     /locus_tag="PK518_09715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRM49233.1"
                     /translation="MAIAQSALPMMGDGAEMTSSAERRLGDGIIRELYRDPDYIDDAV
                     LQEYVQQLFNALVQAAKARGELSPELEERFAWAILLGRDRTVNAFALPGGYFGVHLGL
                     IAVVGTRDELASVLAHELSHVTQRHIARLMAQQGRQTPLMLGAMILGALAASKNPGAA
                     QALMVGGQALAVQNQLNFSRDMEREADRVGFGLMQPAGFAPQGFVAMFDKLQQANRIN
                     DNGSWPYLRSHPLTTQRIADMHSRIPQDATPLASASLEQLMMAARARVLMRPGVDTWR
                     QWVAEPQDAGFAARPRPQRVAAWYAAALSAVQLADPAAARQALRGLQGEVAGDAA"
     gene            1187..1672
                     /gene="moaC"
                     /locus_tag="PK518_09720"
     CDS             1187..1672
                     /gene="moaC"
                     /locus_tag="PK518_09720"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRM49234.1"
                     /translation="MTTPSSPLTHFDAQGQAHMVDVGAKPATRRTAVATGRISMQAAT
                     LALIASGTAKKGDVLGVARIAAIMAAKKTSDLIPLCHPLALTRVAVEFGLLTADNAVQ
                     CTATVETVGPTGVEMEALTAVQVALLTIYDMCKAADKRMCMTGVRVLEKHGGKSGSFV
                     A"
     gene            complement(1689..2477)
                     /locus_tag="PK518_09725"
     CDS             complement(1689..2477)
                     /locus_tag="PK518_09725"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HRM49235.1"
                     /translation="MQVRILGCSGTIAKGCRTTSFLLGEHVLVDAGTGVGQLTLEEMQ
                     RIDHVFLTHSHLDHIAALPLMLDAVGANRRRPLEVHALPQTLAALHAHVFNDVIWPDF
                     TRIPSPAAPFVRLSPLGVGDVFVCAGIPMEVLPARHSVPAVGYAARGAVGWWVYSGDT
                     QGNEPGFWRRVNALHQEGGGVAALLIETAFSNAEADLACKSQHLSPATLMRELAALQG
                     AKPPPVYITHTKPREGRLIMHEVQALDLGALRPVDLQSVQILQV"
     gene            complement(2506..3141)
                     /locus_tag="PK518_09730"
     CDS             complement(2506..3141)
                     /locus_tag="PK518_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRM49236.1"
                     /translation="MPKMVVAIDGVVIKEVPLTQERTTVGRRPYNDVVIDNLAVSGEH
                     AVFVLSGDDVLLHDLGSTNGTYVNGKPVKQQTLRNGDLVEMGKYKIRFLQDAPVSGYD
                     KGLATGLQAPLTGPASMPAPLTGSGGIVRVVTGPAAGREVALTKVVTTIGKPGVAVAS
                     ITKRHHNFVLAHVDGAQLPRLNGAIIGADPVALKSGDRIALAGAEMEFVQG"
     gene            complement(3199..4017)
                     /locus_tag="PK518_09735"
     CDS             complement(3199..4017)
                     /locus_tag="PK518_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRM49237.1"
                     /translation="MKSSRQLGYEFFALTDTGRVRSNNEDAVAIHEAARLVLLADGMG
                     GYSAGEVAADMAVNLVGSELAQWLEDAPERVSMAAVRQAMLVCVENANQAILGAALAN
                     PQYLGMGTTLVVGVFRGHRLILGHVGDSRCYRLRGGELRQITRDHSWLQEQIDLGLLT
                     PAQAAVSGLGNLVTRALGVDGEAHAEINEFAVEPQDLFILCSDGLSDMVGDEGLAELA
                     LLPIALPDKAARMVAMANDRGGRDNISVLLAQAGPGGGGAASKRGLVSRLLRSR"
     gene            complement(4323..5216)
                     /gene="sucD"
                     /locus_tag="PK518_09740"
     CDS             complement(4323..5216)
                     /gene="sucD"
                     /locus_tag="PK518_09740"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRM49238.1"
                     /translation="MSIYINKDTKVITQGITGKTGQFHTEKCQEYANGKNCFVAGVNP
                     KKAGESIFNIPIYASVKEAATQTGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEG
                     IPVRDMLEVRNKMKAKEAAGGKRTLLLGPNCPGLITPDEIKIGIMPGHIHKKGRIGVV
                     SRSGTLTYEAVAQLTELGIGQSSAVGIGGDPINGLKHIDVMRAFNEDPDTDAVIMIGE
                     IGGPDEAEAAQWCKANMKKPIVGFIAGVTAPPGKRMGHAGALISGGADTADAKLAIME
                     ECGFHVTRNPSEMGRILKSLL"
     gene            complement(5234..6394)
                     /gene="sucC"
                     /locus_tag="PK518_09745"
     CDS             complement(5234..6394)
                     /gene="sucC"
                     /locus_tag="PK518_09745"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRM49239.1"
                     /translation="MKIHEYQGKEILRSFGVPVPRGIPAFTVQEAVEAAQKLGGPVWV
                     VKAQIHAGGRGKGGGVKVAKSIDDVKALAGQILGMQLVTHQTGPEGQKVRRLYIEDGA
                     DIKNELYVSLVTDRATQKVALIASSEGGMDIEEVAHSTPEKIITEMIDPLTGITEAQS
                     RKVAAAIGLTGASIDQAVDLFAKIYKCYMDTDASLVEINPLNCNSKGELMALDAKFNF
                     DSNALFRHPEIVALRDLDEEDPAEVEASKFDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGGEPANFLDVGGGATPEKVTEAFKIMLKNPKVKGILVNIFGGIMKCDTIATGVIT
                     ACKAVNLQVPLVVRMKGTNEELGKKMLAESGLPIISADTMAEAATEIVKAVA"
     gene            6567..7499
                     /gene="argC"
                     /locus_tag="PK518_09750"
     CDS             6567..7499
                     /gene="argC"
                     /locus_tag="PK518_09750"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRM49240.1"
                     /translation="MPKVFIDGEAGTTGLQIRERLQAMPAVELVSIAPERRKDAGAKR
                     ELIAGVDLVVLCLHDDAARETVALVDAITAETGKAIKIIDASTAHRTAPGWVFGFPEL
                     AAGQAQAVRGATRVANPGCYATGAIALLRPLVDAGLIAPDQALSLPSVSGYSGGGRSM
                     IEAYEAGQAAPFELYALGLAHKHLPEIMRYTGLTRRPIFVPAVGNFRQGMLVELPLHL
                     DLLPGSPKATDLHDALAAHYAQSNTPEQWVKVLPATEDGKLDALALNDSNQLELRVFA
                     NEAYHHAVLVARLDNLGKGASGAAVQNLKLMLEL"
     gene            complement(7600..8742)
                     /gene="dprA"
                     /locus_tag="PK518_09755"
     CDS             complement(7600..8742)
                     /gene="dprA"
                     /locus_tag="PK518_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRM49241.1"
                     /translation="MQRDELAAWLRLALTPGVGNGAARRLLAAFGLPAQIFARGESAW
                     QSCVTAAQARALATIPQELEALLQTTWQWLQTEGEPARAIVTLGDAAYPPALLATEDP
                     PLLLYLLGAPRFVHGAGPFAPPRCLAMVGSRNPTAQGAEHARQFARVLRAAGLTIVSG
                     LALGVDAAAHEGALLDAPDPDTPATIAVVGTGLDRVYPRANKDLAHRIARHGLLVSEY
                     PLGTPPLPANFPKRNRIISGLSQGTLVVEAALQSGSLITARMAAEQGREVFAIPGSIH
                     APQSRGCHALLRQGAKLVESAEDVLEELRLPAAAPAPAGDAPDATTPAQQEVLQALGF
                     DPLGLDALVARTGLDAATLQVRLLELELDGRVARLPGGLFQRLGAA"
     gene            complement(8772..9968)
                     /locus_tag="PK518_09760"
     CDS             complement(8772..9968)
                     /locus_tag="PK518_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRM49242.1"
                     /translation="MTASGFARAAALGVLAAATLLAPAAQAQNYPVTPGQRATAQQVA
                     ARGVPLAELAPNAPDTYVIRRGDTLWGISGMYLKRPWRWPELWGMNLQAIANPHLIYP
                     GQTLYLEKADGHARLSTSRGGAGETVRLSPRTRHDSLAGMALPTLKMHLIEPFLVQPL
                     VVDELTLAQAPRIVATRDERVIMGNGDRAYVRGPQGSPLEMTPEVPRQYRVFRNALPL
                     KDPNTGEVLGYEAQYVGRAELVRGESLEQSAGTKGEMRTDIVPATVDLSGVKEEARLG
                     DRLLPAPPRSFDNYAPHAPEAPVEASVVSIYGGVAAGNAGPNQVIAINRGTDDGMQAG
                     HVLQLRTGGLRIKDKTDEQRAEIRLPSEDNGLAMVFLTFERVSYALILQAQRGVVVGD
                     RLVNPE"
     gene            10180..10692
                     /gene="def"
                     /locus_tag="PK518_09765"
     CDS             10180..10692
                     /gene="def"
                     /locus_tag="PK518_09765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRM49243.1"
                     /translation="MAILPILCYPDPRLHKIAQPVAVVDERVRAIVDDMFATMYDASG
                     IGLAATQVDVHERIVVIDVSEERDQPLVLINPEIVWASDETRMGDEGCLSVPGIYDGV
                     ERALAVHVRALDRDGQTRVIEAEDLLAVCVQHEMDHLLGKVFVEYLSPLKRTRIKTKL
                     LKQQKQAARA"
     gene            10689..11171
                     /locus_tag="PK518_09770"
     CDS             10689..11171
                     /locus_tag="PK518_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49244.1"
                     /translation="MSRLAAAPLLALALAACAVPEWQKPGTPLADIERTMGRPTLAEP
                     LESGGQRLVYSRQPAGQQVYHMDFDASQRLVRVTQVLTLENFQALRNGLDTQDSVRRR
                     FGPPALLERVASFDGPIWTYRILDNGAPRQAHVHIDPAGVVRRVMFTDERLRDLEPPH
                     "
     gene            11183..12229
                     /gene="fmt"
                     /locus_tag="PK518_09775"
     CDS             11183..12229
                     /gene="fmt"
                     /locus_tag="PK518_09775"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRM49245.1"
                     /translation="MRVIFAGTPEFARVALEHLLAAGFTVPLVLSQPDRPAGRGMKLQ
                     ASPVKQCALEHGIAVAQPRSLRLDGKYPEDAAAAQAAIAAAQADVMVVAAYGLILPQW
                     VLDAMSAARPPEGAKAPLGGSEPRAAGSVGAQGLGCLNIHASLLPRWRGAAPIHRAIE
                     AGDAETGVTIMQMDAGLDTGDMLLCEATAIAPADTQALLHDRLADMGGRLIVQALRQA
                     ANGGLVATPQPAEGITYAHKIDKAESLIDWSQSAEVIARRVRAFDPFPGASTQLGAEV
                     IKVWNCEIDSCQRPSDFRYGQILHIDNNGITVACGAGDVLRLTRLQRAGGKRLPAADF
                     LRGFALAPGMVLGA"
BASE COUNT         1950 a         4190 c         4122 g         2061 t
ORIGIN      
        1 ggcggcgtct cccgccacct ccccctgcaa gccacgcagc gcctggcgcg cggccgcagg
       61 gtcggccagc tgcacggcgc tgagcgcggc cgcataccag gctgccacgc gctggggccg
      121 gggcctggcg gcgaagccgg catcctgcgg ctcggccacc cattggcgcc aggtgtccac
      181 acccgggcgc atcagcacgc gcgcgcgcgc ggccatcatc agttgctcca gcgaggcgct
      241 ggccagcggc gtggcatcct gcggaatgcg gctgtgcatg tcggcgatac gctgcgtggt
      301 cagcgggtgg ctgcgcagat agggccagct gccgttgtcg ttgatccggt tggcctgctg
      361 cagcttgtcg aacatggcga cgaagccctg gggtgcaaaa ccggccggct gcatcaggcc
      421 aaagcccacg cggtcggcct cgcgttccat gtcgcgtgaa aagttgagct ggttctgcac
      481 cgccagcgcc tgtccgccca ccatcagcgc ctgggccgca cccggatttt tgctcgcggc
      541 cagggcgccc agaatcatgg ctcccagcat cagcggcgtc tgccggccct gctgcgccat
      601 caggcgtgcg atatggcgct gcgtcacatg gctgagctcg tgcgccagca ccgaggccag
      661 ttcgtcgcgc gtgccaacca cggcgatcag gcccaggtgc acgccaaagt agccgcccgg
      721 cagggcaaac gcattcaccg tgcggtcgcg cccgagcagg atcgcccagg caaagcgctc
      781 ctccagctcg ggcgagagct cgccacgcgc cttggcggcc tgcaccaggg cgttgaacag
      841 ctgctgcaca tactcctgca gtacggcgtc gtcgatgtag tccgggtcgc gatacagctc
      901 gcggatgatg ccgtcaccca gcctgcgctc ggcgctgctc gtcatctcgg cgccgtcgcc
      961 catcatgggc agcgcggact gtgctatggc cataggtgtc tggcctgcgc cgaatgctat
     1021 taaaatagca gctgctagcg cccttccagc aagcgcaaaa ggcttttttt gcttgatact
     1081 ccgcactcca ctcccctgtc tggagaaccg gactggccgc cgggtcatgg ccgtatgatg
     1141 ccccggccgt gagaatgttt cgcgcccttt tgtaaacccg cccgccatga ccacaccctc
     1201 ctccccgctg acccacttcg acgcccaggg ccaggcccat atggtggacg tgggggccaa
     1261 gcccgccacg cgccgcacgg ccgtcgccac gggccgcata tcgatgcagg ccgccaccct
     1321 ggcgctgata gcaagcggca cggccaaaaa gggcgacgtg ctcggcgtgg cgcgcatcgc
     1381 cgccatcatg gctgccaaga aaaccagcga cttgattccg ctgtgccacc ccctggcgct
     1441 gacccgcgtg gccgtggagt tcgggcttct gaccgccgac aacgccgtgc agtgcaccgc
     1501 gaccgtcgag accgtggggc ccacaggggt ggagatggag gccctgaccg ccgtgcaggt
     1561 ggccctgctg acgatctacg acatgtgcaa ggcggcggac aaacgcatgt gcatgaccgg
     1621 cgtgcgtgtg ctggagaagc atggcggcaa gtccggcagc tttgtggcct gacgcccccg
     1681 ggatagcttc aaacctgcag tatctgcacg ctctgcagat ccacgggccg cagcgcaccc
     1741 aggtccagcg cctgcacctc gtgcatgatg aggcggcctt cgcgcggctt ggtatgggtg
     1801 atgtagacgg gcggcggctt ggccccctgc agggccgcca gctcccgcat gagggttgcc
     1861 ggcgacaaat gctggctctt gcaggccaga tcggcctcgg cattgctgaa ggcggtctcg
     1921 atcaacagtg cggccacgcc gccgccctcc tggtgcaggg cgttcacgcg gcgccagaat
     1981 cctggctcat tgccctgggt atcgccgcta tagacccacc aaccgacagc gccacgcgcg
     2041 gcgtagccca cggcgggcac gctatggcgt gcgggcagca cctccatcgg gatgccggcg
     2101 cagacgaata catcgccgac gcccaggggc gatagccgca cgaaaggtgc ggcaggcgac
     2161 ggaatgcgcg tgaaatcggg ccagatcacg tcgttgaaga catgggcgtg cagcgccgcc
     2221 agggtctgcg gcaaggcgtg cacctccagc ggccggcggc ggttcgcgcc caccgcgtcc
     2281 agcatcagcg gcagggcggc gatgtggtcg aggtgcgaat gcgtgaggaa cacatggtcg
     2341 atgcgctgca tttcctccag cgtgagctgg cccacgccgg tacccgcgtc gaccaggaca
     2401 tgctcgccca gcagaaacga ggtggtgcgg caccccttgg cgatcgtacc gctgcagccg
     2461 aggatgcgta cttgcatgtg ccgggttgtc aaggctgcgg catgctcagc cttgcacgaa
     2521 ctccatctcg gcgccggcca gcgcaatgcg atcgccgctc ttgagcgcca cggggtcggc
     2581 gccaatgatc gcgccattga gccggggcag ctgcgcgcca tccacatggg ccagcacgaa
     2641 gttgtgatgc cgcttggtga tcgaggccac ggcgacgccg ggcttgccta tggtggtgac
     2701 gaccttggtc agcgccactt cgcgcccggc cgccggcccc gtcaccacgc gcacgatgcc
     2761 gccgctgccc gtcaggggcg caggcatgga tgcaggcccg gtcaagggcg cctgcaggcc
     2821 ggtggccagg cccttgtcgt agccggacac cggtgcgtct tgcaggaaac ggatcttgta
     2881 cttgcccatc tccaccagat cgccattgcg cagggtctgc tgcttgacgg gcttgccgtt
     2941 gacataggtg ccgttggtgc tgcccaggtc atgcagcagc acatcgtcgc cggacaggac
     3001 gaacaccgca tgctcaccgc tgaccgccag gttgtcgatg accacgtcgt tgtacggcct
     3061 gcggcccacg gtcgtgcgtt cctgcgtgag cgggacttcc ttgatgacga ctccgtcgat
     3121 agccacgacc attttcggca tgatccgttt tcctcctggg tgttctggtt ccgattgtgc
     3181 cgtatcgctg gcgccaccct agcgcgaacg caacaggcgc gagaccaggc cgcgcttgct
     3241 ggctgcgccg cccccgccag ggccggcttg ggccaaaagg acgctgatgt tatcgcgccc
     3301 accccggtca ttggccatgg ccaccatgcg tgctgccttg tcgggcaggg caatgggcag
     3361 cagcgccagc tcggccaggc cttcgtcgcc caccatgtcg ctcagaccat cggagcacag
     3421 aatgaacaaa tcctgcggct ccacggcaaa ttcattgatt tcagcgtgcg cctcgccatc
     3481 cacgcccagg gcccgggtga ccaggttgcc cagccccgac acggcggcct gcgcgggggt
     3541 cagcaggccc aggtcgatct gctcctgcag ccaggaatgg tcgcgcgtga tctggcgcag
     3601 ctcgccacca cgcaggcggt agcagcgcga gtcgccgaca tgacccagga tgagccgatg
     3661 gccgcgaaac acgccgacca ccagcgtggt gcccatgccc aggtactgcg ggttggccaa
     3721 tgccgccccc agaatggcct ggttggcgtt ttccacgcag accagcatgg cctggcgcac
     3781 cgccgccatc gagacgcgct cgggcgcgtc ctccagccac tgcgccagct cgctgccaac
     3841 caggttgacg gccatgtcgg ccgccacctc accggcgctg tagcccccca tgccatcggc
     3901 cagcagcacc agccgcgcgg cttcatgaat ggcgacggca tcctcattgt tggagcgcac
     3961 acgccccgta tcggtgagcg caaagaactc gtagccgagc tgacgcgagg acttcatgaa
     4021 gccccccgaa acccatggcg aaaggggaca acccgggcgt tctgcctggc cgtgaccgtc
     4081 tgcgcaaagc ccatcatgtt gccaactgat gcaatatgtt acacaaggcg tggatcatag
     4141 actgaggccg gctgtttgcg cgcatggccc ggcatttttt ttgcacggca tgtgggcatt
     4201 cgccgcgcca tgctgtcggc gtcacgcatg gcccaggcca gaaggcacaa cgccagcccg
     4261 gtgttcgggc tggcgttgtc gatagcgggc aagcgcccag gactgtcctg ggcgctacgc
     4321 catcacagca ggctcttgag gatgcggccc atctccgacg ggttgcgcgt cacgtggaag
     4381 ccacactctt ccatgatggc cagcttggcg tcggccgtgt cggcgccgcc cgagatcagc
     4441 gcgccggcgt ggcccatgcg cttgccggga ggggcggtga cgccggcgat gaagccaacg
     4501 atgggcttct tcatgttggc cttgcaccac tgggcggcct cggcctcgtc ggggccgccg
     4561 atttcgccga tcatgatcac ggcgtcggta tccgggtctt cgttgaaggc gcgcatcaca
     4621 tcgatgtgct tgaggccgtt gatcgggtcg ccgccaatgc ccacggccga cgactggcca
     4681 atgcccagct cggtcagctg cgccacggct tcataggtca gcgtgccaga gcggctgacc
     4741 acgccgatgc ggcccttctt gtggatatgg ccgggcatga tgccgatctt gatctcgtcg
     4801 ggcgtgatca ggccggggca gttggggccg agcagcagcg tgcgcttgcc gccagcggcc
     4861 tccttggcct tcatcttgtt gcgcacttcc agcatgtcgc gcaccgggat gccttcggtg
     4921 atgcagatgg ccagatccag gtcggcctcg acggcttccc agatggcggc ggcggcaccg
     4981 gccggcggca cgtagatcac cgacacggtg gcgccggttt gcgtggcggc ttccttgacg
     5041 ctggcgtaga tcgggatgtt gaagatggac tcaccggcct tcttcgggtt cacgccggcg
     5101 acgaagcagt tcttcccgtt ggcgtattcc tggcacttct cggtgtggaa ctggccggtc
     5161 ttgcccgtga tgccctgggt aatgaccttc gtgtctttat tgatgtagat ggacatgttg
     5221 ttctctccag gccttaagcg acggctttga cgatctcggt ggccgcttcg gccatggtgt
     5281 cggcgctgat gatgggcagg ccggattcgg ccagcatctt cttgcccagc tcttcgttcg
     5341 tgcccttcat gcgcacgacc agcggcactt gcaggttcac ggccttgcag gcggtgatga
     5401 cgccggtggc gatggtgtcg cacttcatga tgccgccgaa gatattgacc agaatgccct
     5461 tgaccttggg gttcttgagc atgatcttga aggcttcggt gaccttctcg ggggtggcgc
     5521 cgccgcccac gtccaggaag ttggccggct cgccgccgaa cagcttgatg gtgtccatgg
     5581 tggccatggc caggccggcg ccgttgacca ggcagccgat gttgccgtcc aggctgatgt
     5641 aggccaggtc gaacttggaa gcctcgacct cggccgggtc ttcttcgtcc aggtcacgca
     5701 gggcgacgat ctcggggtgg cggaacagcg cgttgctgtc aaagttgaac ttcgcgtcca
     5761 gcgccatcag ttcgcccttg gagttgcagt tcagcgggtt gatctccacc agggacgcat
     5821 ccgtgtccat gtagcacttg tagatcttgg caaagagatc cacggcctgg tcgatggagg
     5881 cgccggtcaa accgatggcg gccgccacct tgcgcgactg ggcttcggtg atgccggtga
     5941 gcgggtcgat catctcggtg atgatcttct cgggcgtgct gtgcgccact tcctcgatgt
     6001 ccatgccgcc ctcggacgag gcgatcagcg ccaccttctg cgtggcgcgg tcggtgacca
     6061 gcgaaacgta gagttcgttc ttgatgtcgg cgccgtcttc gatgtacagg cggcgtacct
     6121 tctggccttc ggggccggtc tggtgcgtga ccagctgcat gcccagaatc tggccggcca
     6181 gtgccttgac gtcgtcaatg ctcttggcga ccttgacgcc gccgcccttg ccacggccac
     6241 cggcgtggat ctgggccttg accacccaca cggggccgcc gagcttttgt gcggcttcca
     6301 cggcctcttg caccgtgaac gcaggaatgc cacgcggaac gggcacgccg aaactacgca
     6361 agatttcctt gccttggtat tcgtgaatct tcatgaggtc tctctcaggg gaaggatggt
     6421 gtttgtaccc gttctgccga tgtgcagaaa cttccgcatg cccgggaatg gcagccagca
     6481 attgtattat gttgcgatgc accattgctt ggtgttaact tacaccccgg ccgcagtccc
     6541 caatcggccc ttgcaggagc tcttcaatgc ccaaggtttt tatcgatggc gaggcaggca
     6601 ccaccggcct gcagatccgt gaacgcctgc aggccatgcc tgcggtggag ctggtcagca
     6661 tcgcgcccga acggcgcaag gatgccggcg ccaagcgcga gctgatcgcc ggcgtggatc
     6721 tggtcgtgct gtgcctgcac gacgatgccg cgcgcgaaac cgtcgctctg gtggacgcca
     6781 tcacggcgga gaccggcaag gccatcaaga tcatcgacgc ctccaccgcg caccgcacgg
     6841 cgccgggctg ggtgttcggc tttcccgagc tggccgccgg ccaggcgcag gccgtgcgcg
     6901 gcgccacgcg cgtggccaac ccgggctgct acgccacggg cgccatcgcc ctgctgcgcc
     6961 cgctggtcga tgccggtctg atcgcccccg accaggcgct gtccctgccc tcggtgagcg
     7021 gatactccgg cggtggccgc agcatgatcg aagcctacga ggccggccag gccgccccgt
     7081 ttgagctcta tgccctgggc ttggcgcaca agcatctgcc ggagatcatg cgctacacgg
     7141 gcctgacgcg ccggcccatc ttcgtgcccg cagtgggcaa cttccgccag ggcatgctgg
     7201 tggagctgcc gctgcacctg gatctgctgc caggcagccc taaggccacc gacctgcacg
     7261 acgcactggc cgcacactac gcgcaaagca acaccccgga gcaatgggtg aaggtgctgc
     7321 cggccaccga ggacggcaag ctcgacgcgc tggcgttgaa tgacagcaac cagctggagc
     7381 tgcgcgtgtt cgccaatgag gcctatcacc atgccgtgct ggtggcgcgc ctggacaacc
     7441 tgggcaaggg cgccagcggc gccgccgtgc aaaacctcaa attgatgctg gaactataaa
     7501 aagaatagct ttttgcgctt gctatttggc gaattcaggc aaagtttcgc atgaatatgg
     7561 ctaacaacaa tcgctgatag cttctgtttt tatagcaatt caggccgcgc ccaggcgctg
     7621 aaacaagccg cccggcaggc gcgccacgcg gccgtcgagc tccagctcca gcagccgcac
     7681 ctgcaacgtt gcggcatcca gcccggtgcg cgccaccagc gcgtccagcc ccagcgggtc
     7741 aaagcccagc gcctgcagca cctcctgctg cgccggcgtg gtggcgtctg gcgcatcgcc
     7801 cgccggcgcc ggcgcagcgg cgggcaggcg cagctcctcc agcacatcct cggccgactc
     7861 caccagcttg gcgccctggc gcagcaatgc atggcagccg cgcgactgcg gcgcatggat
     7921 ggagccgggg atggcaaaca cctcgcgccc ctgctcggcc gccatgcgcg cggtgatcag
     7981 cgagcccgac tgcagcgccg cctcgaccac cagcgtgccc tgtgacaggc cggagatgat
     8041 gcggttgcgc ttgggaaagt tggccggcag cggcggcgtg cccagcgggt actcgctgac
     8101 cagcaggcca tggcgcgcga tgcggtgcgc cagatccttg ttggcgcgcg ggtagacgcg
     8161 atcgagcccg gtgccgacca cggctatggt ggccggcgtg tccgggtcgg gcgcatccag
     8221 cagcgccccc tcgtgcgccg ccgcatccac ccccagagcc agaccggaga cgatggtcag
     8281 ccccgccgcg cgcaacacgc gcgcaaactg gcgcgcatgc tcggcgccct gggccgtggg
     8341 gttgcggctg cccaccatgg ccaggcagcg cggcggcgca aatggcccgg cgccatgcac
     8401 gaagcgcggc gcgcccagca ggtacagcag cagtggcggg tcttcggtgg ccagcagggc
     8461 cgggggatag gcggcatcgc ccagggtgac gatggcgcgc gccggctcgc cctcggtctg
     8521 cagccattgc caggtggtct gcagcagggc ctccagctcc tgtgggatgg tggccagggc
     8581 gcgtgcctgg gcggccgtca cgcaggactg ccaggcggat tcgccccggg caaagatctg
     8641 cgccggcagg ccgaaggcgg ccagcaggcg ccgcgccgcg ccgttgccca cgccgggcgt
     8701 gagggcgagg cgcagccagg cggcgagttc gtcgcgctgc atggcctgaa actcggcgcc
     8761 gcggcccgcg cctactcggg gttgaccagc cggtcgccca cgaccacgcc gcgctgcgcc
     8821 tgcaggatga gcgcatagga cacgcgctcg aaggtcagga acaccatggc cagtccgttg
     8881 tcctcgctgg gcaggcggat ctcggcgcgc tgctcgtcgg tcttgtcctt gatgcgcagg
     8941 ccgccggtgc gcagttgcag cacatggccg gcctgcatgc cgtcgtcggt gccgcggttg
     9001 atggcgatga cctggttcgg cccggcattg cccgccgcga cgccgccgta gatggacacc
     9061 acgctggcct ccaccggcgc ctcgggcgca tgcggtgcgt aattgtcaaa gctgcgcgga
     9121 ggcgcgggca gcaggcggtc gccaaggcgc gcttcttcct tcacgcccga caaatcgacc
     9181 gtggcgggca cgatgtcggt gcgcatctcg cccttggtgc cggcagactg ctccagcgac
     9241 tcgccgcgca ccagctcggc gcggccgaca tactgcgcct cgtagcccag cacctcgccg
     9301 gtgttcgggt ccttcagcgg cagggcgttg cggaacacgc ggtactggcg cggcacctcg
     9361 ggcgtcatct ccagcgggct gccctgcggg ccgcgcacat aggcgcggtc gccattgccc
     9421 atgatcacgc gctcgtcgcg cgtggccacg atgcgcggcg cctgggccag cgtcagctcg
     9481 tccaccacca gcggctgcac cagaaacggc tcgatcaggt gcatcttcag cgtcggcagg
     9541 gccatgccgg ccaggctgtc gtggcgcgtg cgtggcgaca ggcgcacggt ctcgccggcg
     9601 ccgccgcggc tggtgctcag gcgtgcatgg ccatcggcct tttccaggta cagggtctgg
     9661 ccggggtaga tcaggtgcgg gttggcgatg gcctgcaggt tcatgcccca cagctcgggc
     9721 cagcgccagg gtcgcttcag gtacatgccg gagatgcccc acagcgtgtc gccgcgccgg
     9781 atcacatagg tgtcgggcgc gttgggcgcc agctcggcca ggggcacacc acgcgctgcc
     9841 acctgttgcg cggtggcgcg ctggccaggc gtgacggggt agttctgtgc ctgggccgcc
     9901 ggcgccagca aagtggccgc ggccaggacg ccgagcgcag ccgcgcgcgc gaagcccgaa
     9961 gccgtcatgt ttggattggt ctggtcatgc attggcagat gccccctctc actgcacggg
    10021 cgacatggtt aattttttta caaaagaaac ggtcgcagat actttaaaaa cgatttgaga
    10081 ttttctgcgc tagcccttga atgggcaacg attattgcga gcagcgcctg catcgcgcgg
    10141 gcaaagtggc gaaaatagcg acatatcaag ctcccagcca tggccattct tcccattctt
    10201 tgctaccccg acccgcgttt gcacaagatc gcccagcccg tggcggtggt cgatgagcgc
    10261 gtgcgcgcca tcgtcgatga catgttcgcc accatgtacg acgccagcgg cataggcctg
    10321 gcggccaccc aggtggatgt gcacgagcgc atcgtcgtca tcgatgtctc cgaggagcgc
    10381 gaccagcccc tggtgctgat caaccccgag atcgtgtggg ccagcgacga gacgcgcatg
    10441 ggcgacgagg gctgcctgtc ggtgcccggc atctacgacg gcgtggagcg cgcgctggcc
    10501 gtgcatgtgc gcgcgctcga tcgtgacggc cagacgcgcg tgatcgaggc cgaggatctg
    10561 ctcgccgtct gcgtgcagca cgagatggac catctgctgg gcaaggtgtt cgtggaatac
    10621 ctctcgccgc tcaaacgcac ccgcatcaaa accaagctgc tcaagcagca gaaacaggcc
    10681 gcacgcgcat gagccggctt gcggcggccc cgctgctggc cctggcactg gccgcctgcg
    10741 ccgtgcccga atggcaaaag cccggcacgc ccttggcaga cattgaacgc accatgggcc
    10801 gccccacgct ggccgagccg ctggagagtg gtggccaacg cctggtgtac agccgccagc
    10861 cggcgggcca gcaggtctat cacatggact ttgacgccag ccaacgcctg gtgcgcgtca
    10921 cccaggtgct gacactggag aacttccagg cgctgcgcaa tgggctggac acgcaagaca
    10981 gcgtgcgccg caggttcggc ccgccggcgc tgctggagcg ggtggccagc tttgacggcc
    11041 ccatctggac ctaccgcatt ctggacaatg gcgcgccgcg ccaggcccat gtgcacatcg
    11101 acccggcggg cgtggtgcgt cgcgtgatgt tcaccgacga gcgcctgcgc gatctcgaac
    11161 cccctcactg aatacctgcc gcatgagagt catttttgcc ggcacgcccg aattcgcgcg
    11221 cgtggccctg gagcatttgc tggccgcggg ttttaccgtg cccctggtgc tgagccagcc
    11281 cgaccgcccg gccggccgcg gcatgaagct gcaggcctcg cccgtcaagc aatgcgcgct
    11341 ggagcatggc attgccgtgg cccagccgcg cagcctgcgc ctggacggca aataccccga
    11401 ggacgccgcc gcagcccagg ccgccattgc ggccgcccag gccgacgtga tggtggtcgc
    11461 cgcctatggg ttgatcctgc cgcaatgggt gctggacgcc atgagcgccg cacggccgcc
    11521 cgaaggcgcg aaggcccccc tggggggcag cgaaccacgc gcagcgggga gcgtgggggc
    11581 ccaaggcctg gggtgtttga acatccacgc cagcctgctg ccgcgctggc gcggcgccgc
    11641 gcccatccac cgcgccatcg aggccggcga cgccgagacc ggcgtgacca tcatgcagat
    11701 ggatgccggc ctggacacgg gcgacatgct gctgtgcgag gcaaccgcca tcgcgcccgc
    11761 cgacacccag gccctgctgc acgaccggct ggccgacatg ggcgggcggc tcatcgtgca
    11821 ggcgctgcgc caggcggcga acggcggcct ggtggccacg ccgcagccgg ccgaaggcat
    11881 cacctacgcg cacaagatag acaaggccga gagcctgatc gactggagcc agagcgccga
    11941 ggtgatcgcc cggcgcgtgc gcgccttcga cccctttccg ggcgccagca cccagctcgg
    12001 ggctgaagtc atcaaggtgt ggaactgtga aattgatagc tgtcagcgcc cgtcagactt
    12061 tcgttacggc cagattttgc atatagacaa taacggtatc accgtggcct gcggcgcggg
    12121 cgatgtgctg cgcctgaccc ggctgcagcg cgccggcggc aagcgcctgc cggccgccga
    12181 cttcctgcgc ggctttgcgc ttgcaccggg catggtgctc ggcgcatgaa cctgcccgcc
    12241 ctgcgcgccc agctgcgcga cccgtccttc cgccagggcg ccagcgacat gctgggcgcc
    12301 ggcctgggcg tgggcgcctg ggg
//
LOCUS       DAOMTQ010000090        12242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_934_length_12242_cov_25.080414, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000090 DAOMTQ010000000
VERSION     DAOMTQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_934_length_12242_cov_25.080414"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1688
                     /gene="aceF"
                     /locus_tag="PK518_09780"
     CDS             39..1688
                     /gene="aceF"
                     /locus_tag="PK518_09780"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HRM49246.1"
                     /translation="MALIQIQVPDIGDFDEVGVIELLVKPGDTVALEQSLITVESDKA
                     SMEIPSSHAGVVKALTVKVGDKVKQGSVIVELEAADGGAAAAPAPAAAPTAAAPAPVA
                     APAAAPVAAAAAGPINVHVPDIGDFKDVAVIELLVQVGDAIKVEQSLFTVESDKASME
                     IPSPVAGTLKELKIKVGDKVNIGDLVAVIEGAAAGAASAPASAAPSPAPVAAPASAPA
                     PVAAAATAIAMPAHQPGSPTPGLPHASPSVRKFARELGVPLAELKGTGLKGRITAEDV
                     QAFTRSVMAGAVQTQAQAARAPAGGGSGVGLDLLPWPKVDFAKFGPIERKELSRIKKI
                     SGANLLRNAIVIPAVTNHDDADITDLEAFRVSTNKENEKGGVKVTMLAFLIKACVAAL
                     KKFPEFNSSLDGDALIYKQYYHIGFAADTPNGLVVPVIKDADKKGVLQISQEMGDLAK
                     KARDGKLGPADMSGATFTISSLGGIGGRYFTPIINAPEVAILGVCRSTMEPVWDGKAF
                     HPRLMLPLSLTWDHRVIDGAAAARFNVYLGQILADFRRVLL"
     gene            1702..3567
                     /gene="lpdA"
                     /locus_tag="PK518_09785"
     CDS             1702..3567
                     /gene="lpdA"
                     /locus_tag="PK518_09785"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRM49247.1"
                     /translation="MAVIDIKVPNIGDFAEVGVIEVLVQPGDSVAVEQSLITVESDKA
                     SMEIPSSHAGVVKELKVKVGDKVAEGTVVLTLEAAAASPSPAGAGEGRGEGGVSKATP
                     PAPSPQPSPASGRGGEQPAAASYAGNADLDCDVLVLGGGPGGYSAAFRAADLGLKVVL
                     VERYATLGGVCLNVGCIPSKALLHVAAVMDEVSHLKAAGIDFGAPSVNVDTLRGHKEK
                     VISKLTGGLGQMAKMRKVTIVRGYGHFLGSHHLEVEETTGSGQDKTGAKKVVQFKNAI
                     IAAGSQAVRLPFMPNDPRVVDSTGALALAGVPKRMLILGGGIIGLEMGTVYSTLGARL
                     DVVEMMDGLMQGADRDLVKVWQKMNAPRFDNIMVNTKTVAAEATPEGIKVTFAPAKDG
                     VTVPEPQTYDLVLQAVGRSPNGKKIAADKAGVAVTDRGFIDVDIQMRTNVPHIFAIGD
                     IVGQPMLAHKAVHEAHVAAEVIAGELQGNKELAAAAFNARVIPSVAYTDPEVAWVGLT
                     EDQAKAQGIKVKKGLFPWAASGRAIANGRDEGFTKLLFDDSPEAHGHGKILGGGIVGT
                     HAGDMIGEIALAIEMGADTVDIGKTIHPHPTLGESIGMAAEVAHGSCTDVPPARK"
     gene            3679..4626
                     /locus_tag="PK518_09790"
     CDS             3679..4626
                     /locus_tag="PK518_09790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM49248.1"
                     /translation="MQALLAAIAAMPVATDACRIFHGRGGRFAGCEQWALDAYPPVLV
                     LTSFAPATEEQLAIIGSALQARWQQIAPGQGLNWVFQHRGEALRMAGRSETRLMAGAV
                     PDPHVVTEDGARYRVHVLRGQNHGLFLDMAAGRRWVRAHAAAHRARTGQGARVLNLFA
                     YTCAFSVAALQAGAAQVVNVDMGRGSIATGQHNHQLNGLAGGASFLAHDIFSSWGKIA
                     RGGPYDLVIADPPSYQKGSFVATKDYARIMRRLPDLLAPGGHALLCLNAPELGLAFLR
                     EQMQACAPALDFVARVDNPPVFADVDEQRSLKVLVFKRD"
     gene            4665..5549
                     /locus_tag="PK518_09795"
     CDS             4665..5549
                     /locus_tag="PK518_09795"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM49249.1"
                     /translation="MNTLPVGLVGVGLMGHGIALNIARRGYALTVLEHPGNQPLDELR
                     ALGVDTAESVQALVRRVKVLILCVTGSPEVEAVLLGEQGALAALRPGTVVIDCSTAIP
                     VSTEAVARAVAQAGGRFLDAPMTRTPREAAEGRLNLLVGGDADLLQECLPLLQCFAEH
                     ITHTGAAGSGHRMKLLHNYVSLGSVALIAEAAACALAAGVAPEVFVDVLAKGGGGGVA
                     LERLRPYLLAQDPGGLRFSMSNALKDMGYYSAMARDGEAAHGIAQAVHDSFALAVADG
                     GPQRLVPELVEIFKKKSR"
     gene            complement(5574..6077)
                     /locus_tag="PK518_09800"
     CDS             complement(5574..6077)
                     /locus_tag="PK518_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRM49250.1"
                     /translation="MTTETTKPPRYWLMKSEPDECSIDDALAAAGATVPWTGVRNYQA
                     RNFMRDAMQVGDGVLFYHSSCPEPGIAGIARVASGVRADPTQFDPQSPYYDPKAAPGK
                     PRWLLLDVQALRKTRLVGLAELRAHPALADMRVLQRGNRLSITPVAAREWTAILTDCL
                     GISTTKG"
     gene            complement(6139..6687)
                     /locus_tag="PK518_09805"
     CDS             complement(6139..6687)
                     /locus_tag="PK518_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRM49251.1"
                     /translation="MSRWLIVLLLVSANAYASPPDEVERMLAERGLIGQLQELRHDVA
                     DRTSDLISTAMGFIGVPYRRGGNSAETGFDCSGFVRVIFDQAKGMVLPRKASEQAAAT
                     EVIDKKDLQPGDLVFFNTMRRAFSHVGIYVGDGKFIHAPRSGAQVRVEDMGVAYWARR
                     FNGARRVAANDQETANLAASAR"
     gene            complement(6654..7004)
                     /locus_tag="PK518_09810"
     CDS             complement(6654..7004)
                     /locus_tag="PK518_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49252.1"
                     /translation="MGIKPGRSGRKNQTKSIAEFGRQAMAFLAAFTPKQQANTGTGRK
                     KEKARKNKSKLQQPRRKQKIWVGRQRQASIGLDLDAWNFSRINKFQHFCGGICPLPCY
                     PPPPCLVGLSFCYL"
     gene            7003..8427
                     /gene="cycA"
                     /locus_tag="PK518_09815"
                     /gene_synonym="dagA"
     CDS             7003..8427
                     /gene="cycA"
                     /locus_tag="PK518_09815"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HRM49253.1"
                     /translation="MTFKDAASGQGVQGQHDLQRHLTNRHIQLIAIGGAIGTGLFMGS
                     GKTISLAGPSIVLVYAIIGVMVFFVMRAMGELLLSNLRYKSFIDFASDLLGPWAGFFT
                     GWTYWFCWIVTGIADVIAISAYAQFWFPDLPQWAPALACVGLLLGLNLLTVRLFGEIE
                     FWFAMIKIVAIVTLVGTGVAMVAGGFVSPAGRAASLSNLWNDGGLFPHGAMGFFAGFQ
                     IAVFAFVGIELVGTTAAEAQDPERTLPRAINSIPVRIIIFYVCALIAIMAVTPWRDVV
                     PGKSPFVELFVLAGLPAAAGVINFVVLTSAASSANGGVFSTSRMLYGLALKGDAPRAF
                     YQLSRASVPSRGLLFSCGCLLLGAFLMWVVPDLVQAFTLVTTVSAILFMFVWSLILLS
                     YIAYRRQRPALHAASKYKMPGGVAMCWACLAFFAGILVLLSLETDTRQALVVTPLWFV
                     LLGVAYRFMLRRQPEGATVVSVPS"
     gene            8574..11753
                     /locus_tag="PK518_09820"
     CDS             8574..11753
                     /locus_tag="PK518_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM49254.1"
                     /translation="MFILAARRRLLGHWLPGLALALAGCAASYALWLQQTASMQSIAQ
                     ARFAQETRLFADALQRRMESHAELLQGMRGLLTVNPELRRSEFERVASDLALASSHPG
                     VKNINFTRHVAGDERRAFETRARGDAHLDGSLPTDFAIRPAQERPEYYVVEFLWPRAG
                     NAGILGLEIHSVPEALEALLRARDSAGLTASAPFQLAGSDGDRTGIMLRLPVFAGPAP
                     GDGQARRILGAVGVSVHIETAIHAMRNQGQMHNLSVTLSDIGLAGGVSPAAAERLYQS
                     PSMDASAVDGMQRTEEVLVGGRRWLLQLQPTASFLSSQEQRLPGLLALGGLLVTALLT
                     VSVSMLVLRRARALSDVRQAGTALSESEARFSAVFHQAAVGFVQVDLDSDRLVRMNQK
                     FCDLLGYTEQELQQLRFQDITVAEDLQDDLAQMARLRAGELRDFRMEKRYRRKDGGVV
                     WAELSVSRLRLADGTGYHLAVVQDISERKRMEQALRGSEQRLLGILNHMPVGVNLVQD
                     ERIVFRNASHVQICGYGADEAPDVDSLWSLTIPDAAQREQVRRSWQAACAAARRAAVD
                     GGAAIAPLECVITAKSGVQHIVELSGMMLEDSHIVTMVDLSQRRRAEQEVRYLAYNDP
                     LTGLPNRRLLLDRLQQALAISARHQWCGALLMLDLDNFKQINETLGHDMGDRLLQAVA
                     ERLRACVPDDDTLARHGGDEFVVVLKDLGATPAEAAARAEEAAQKMLQAMRAPFVIDG
                     GEPRHSTLSVGIAVFQGQRESADELLKRSDMAMYEAKAAGRDALRFFDPQMQAQMAER
                     VALEADMRAGLDSGQFELFYQPKMVRGQITGAEALLRWRHPVKGYVSPAEFIPLAEQC
                     GLILRLGQWVLRTACERLAHWQEHPVLGQLTVAVNVSPRQFYEAGFVPQVLEALAGAG
                     ADARRLRLELTEGLLLQDVEDTIAKMVQLRGYGVGFSLDDFGTGYSSLAYLKRLPLHE
                     LKIDQSFVRDVLTDPNDAAIARTIVALGTSLGLQVTAEGVETEAQRLFLERAGCHAWQ
                     GYLLSRPVPGLAFEDLVLAHAAAVGPHVP"
     gene            11787..12116
                     /locus_tag="PK518_09825"
     CDS             11787..12116
                     /locus_tag="PK518_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HRM49255.1"
                     /translation="MQVVTTLALFLATALAEILGCYLPWLWLRQGRSAWLLLPAAASL
                     ALFAWLLTLHPAAAGRVYAAYGGVYVAVALAWLWAVDGIRPGPWDWLGVAVTLCGMAI
                     IAFAPRG"
BASE COUNT         1946 a         3942 c         4205 g         2149 t
ORIGIN      
        1 atcaaccccc tgcacgcctg aagacgaggg agacaaccat ggcattgatt caaatccagg
       61 tacccgatat cggggacttt gacgaagtcg gcgtgatcga attgctggtc aagcccggcg
      121 atacggtggc gctggagcag tcgctgatca cggtcgagtc ggacaaggca tcgatggaga
      181 ttccgtcgag ccacgccggc gtggtcaagg cgctgaccgt gaaggtgggc gacaaggtca
      241 agcagggcag cgtgatcgtt gagctggagg ccgccgatgg cggtgccgcc gctgctcccg
      301 cgcccgcagc ggcgcccact gccgccgcgc cggccccggt ggctgccccc gctgcggccc
      361 ccgttgcagc agcagctgct ggccccatca acgtgcatgt acccgacatc ggcgacttca
      421 aggacgtggc cgtgatcgag ctgctggtcc aggtgggtga tgccatcaag gtcgagcagt
      481 cgctgttcac cgtcgagtcc gacaaggcgt cgatggagat tccctcgccc gtggccggca
      541 cgctcaagga gctgaagatc aaggtgggcg acaaggtcaa catcggtgac ctggtggccg
      601 tcatcgaagg cgccgcggcc ggcgctgcgt cagcaccagc cagcgcagct ccaagcccgg
      661 caccggttgc agcacctgca tccgcgccgg cacctgtggc cgctgccgcc accgctattg
      721 ccatgcccgc gcaccagccc ggcagcccca cgccgggcct gccccacgca tcgccgtcgg
      781 tgcgcaagtt cgcccgcgaa ctcggcgtgc cgctggccga gctcaagggc acgggcctca
      841 agggccgcat cacggccgag gatgtgcaag ccttcacccg ctccgtcatg gcaggcgccg
      901 tgcaaaccca ggcccaggcc gccagggccc cggccggagg tggctcgggt gtgggtctgg
      961 atctgctgcc ctggcccaag gtggacttcg ccaagttcgg ccccatcgag cgcaaggagc
     1021 tgtcccgcat caagaagatc agcggcgcca acctgctgcg caatgccatc gtgattccgg
     1081 ccgtcaccaa ccatgacgat gccgacatca ccgacctgga agccttccgc gtctccacca
     1141 acaaggagaa cgagaagggc ggcgtcaagg tcaccatgct cgccttcctg atcaaggcct
     1201 gcgtcgcggc gctgaaaaaa ttccccgagt tcaacagcag cctggacggc gatgcgctga
     1261 tctacaagca gtactaccac atcggttttg ccgccgatac gcccaatggc ctggtcgtgc
     1321 ccgtgatcaa ggacgccgac aagaagggcg tgctgcagat cagccaggaa atgggggatc
     1381 tcgccaagaa ggccagggat ggcaagctgg gcccggccga catgagcggc gccaccttca
     1441 ccatctccag tctgggcggc attggcgggc gttatttcac gcccatcatc aacgcgcccg
     1501 aggtggccat cctgggcgtg tgccgctcga ccatggagcc ggtgtgggac ggcaaggcct
     1561 tccatccgcg cctgatgctg ccgctgtcgc tcacctggga tcaccgcgtg atcgatggtg
     1621 ccgcagccgc gcgcttcaac gtctacctgg gccagatcct ggcggacttc cgccgcgtgc
     1681 tgctctgaaa ggaatactga catggcagtc atcgacatca aggtgcccaa tattggcgac
     1741 ttcgccgagg ttggcgtgat cgaagtgctg gtgcaacccg gcgacagcgt ggccgtcgag
     1801 caatcgctca tcaccgtgga gagtgacaaa gcctccatgg aaatccccag cagccatgcc
     1861 ggggtggtga aagagctcaa ggtcaaggtg ggcgacaagg tggcggaagg gacggtggtg
     1921 ctgacgttgg aggcagccgc tgcttctccc tctcccgccg gggcgggaga gggcaggggt
     1981 gagggtgggg tcagcaaggc cacgccgcct gcaccctcac cccaaccctc tcccgcgagc
     2041 gggagagggg gcgaacaacc agcggccgcc agctacgccg gcaacgccga cctcgactgc
     2101 gacgtgctcg tgctgggcgg cggtcccggc ggctattcgg cggcgttccg cgccgccgac
     2161 ctgggcctga aggtggtgct ggtcgagcgc tacgccacgc tgggcggcgt gtgcctgaac
     2221 gtgggttgca tcccatccaa ggccttgctg catgtggctg cggtgatgga tgaggtcagc
     2281 cacctgaagg ccgcgggcat cgacttcggc gcgcccagcg tcaacgtcga taccctgcgc
     2341 ggccacaagg aaaaggtgat ctccaagctc accggcggcc tgggtcagat ggccaagatg
     2401 cgcaaggtca cgatcgtgcg cggctatggg catttcctcg gcagccacca cctggaggtg
     2461 gaggaaacga caggcagcgg ccaggacaag accggcgcca agaaggtcgt gcagttcaag
     2521 aacgccatca tcgccgccgg cagccaggcc gtgcgtctgc ccttcatgcc caacgacccg
     2581 cgcgtggtgg acagcaccgg cgcgctggcg ctcgctggcg tgccaaaacg catgctgatc
     2641 ctgggcggcg gcatcatcgg cctggagatg ggcacggtgt attccacgct gggcgcgcgc
     2701 ctggacgtgg tcgagatgat ggacggcctg atgcagggcg ccgaccgtga tctggtcaag
     2761 gtctggcaga agatgaacgc gccgcgcttc gacaacatca tggtcaacac caagaccgtg
     2821 gcggccgagg ccacgccgga aggcatcaag gtcacgttcg ctcctgccaa ggacggcgtc
     2881 acggtgcccg agccgcagac ctacgacctg gtgctgcagg ccgtgggccg ctcgcccaac
     2941 ggcaagaaga tcgccgccga caaggcgggg gtggcggtga cggatcgcgg cttcatcgac
     3001 gtggacattc agatgcgcac caacgtgccg catatctttg ccatcggcga catcgtcggc
     3061 cagcccatgc tggcgcacaa ggcggtgcac gaggcccatg tggcggccga ggtcatcgcc
     3121 ggcgaactgc agggcaacaa ggagttggcg gcagccgcct tcaacgcccg cgtcatcccc
     3181 agcgtggcct acaccgaccc cgaggtcgcc tgggtcggcc tgaccgaaga ccaggccaag
     3241 gcccagggca tcaaggtcaa gaagggcctg ttcccatggg cggcctccgg ccgggcgatt
     3301 gccaacggcc gcgacgaggg cttcaccaag ctgctgttcg acgattcacc cgaggcccac
     3361 ggccacggca agatcctggg cggcggcatc gtcggcacgc acgccggcga catgatcggc
     3421 gagattgccc tggccatcga aatgggcgca gacacggtgg acatcggcaa gaccatccat
     3481 ccgcacccca cgctgggcga gagcataggc atggcggccg aggtggcgca tggcagctgc
     3541 accgacgtgc cgccggcgcg caagtaggca caatcgcccc gatgaaaagg cagctttggc
     3601 tgcctttttc tatggttttt atagcttttg acgcttgctg tataagctct gggccttctt
     3661 tttatctcaa atcacgccat gcaagccctg ctcgccgcca ttgccgccat gcctgtcgcc
     3721 acggatgcct gccgcatctt ccacggccgg ggcgggcgct ttgccgggtg cgagcagtgg
     3781 gcgctggacg cctatccgcc ggttttggtg ctgaccagct ttgcgccggc cacggaggag
     3841 caactggcca tcatcggcag cgcgctgcag gcccgttggc agcagatcgc tcctggtcag
     3901 ggcttgaact gggtgttcca gcaccgtggc gaggcgctgc gcatggcggg tcgcagcgaa
     3961 acgcggctga tggccggcgc cgtacctgat ccgcatgtgg tcaccgagga cggcgcgcgc
     4021 tatcgcgtgc atgtgctgcg cgggcagaac cacggcctgt tcctggacat ggcggcgggc
     4081 cggcgctggg tgcgcgcgca tgccgccgcg catcgggcgc gcaccgggca gggcgccagg
     4141 gtgctgaacc tgttcgccta cacctgcgcc ttctcggtcg cggcgctgca ggccggcgcg
     4201 gcccaggtgg tcaacgtgga catgggccgg ggatcgattg ccacggggca gcacaaccac
     4261 cagcttaatg gcctggccgg cggcgccagc tttctggcgc atgacatctt cagctcctgg
     4321 ggcaagatcg cgcgcggcgg gccgtacgac ctggtgatcg ccgacccgcc cagctatcag
     4381 aagggcagct ttgtcgccac caaagactac gcgcgcatca tgcgccgcct gcccgatctg
     4441 ctggcgcccg gcggccatgc gctgctgtgc ctgaatgcgc cggagctggg cctggccttt
     4501 ctgcgcgagc agatgcaggc atgcgcgcct gcgctggatt ttgtcgcgcg cgtggacaat
     4561 ccgccggtgt ttgccgacgt cgatgagcag agatccttga aggtgctggt tttcaagcgc
     4621 gactgaagcc gctggctaca acccattacg caaaagggcc atccatgaac accttacctg
     4681 tcggcctggt cggcgtcggc ctgatgggcc atggcattgc gctgaacatc gcgcgcaggg
     4741 gctacgccct gacggtgctg gagcaccccg gcaaccagcc gctggatgag ctgcgtgccc
     4801 tgggtgtgga cacggcagag tcggtgcagg cgctggtgcg gcgggtcaag gtgctgatcc
     4861 tgtgcgtgac cggctcgccc gaggtcgagg ccgtgctgct gggcgagcag ggcgcgctgg
     4921 ccgcgttgcg gcccggcacg gtggtgatcg actgctcgac ggccattccc gtgtctaccg
     4981 aagcggtagc gcgcgccgtg gcccaggcgg gcgggcgctt tctggatgcg cccatgacgc
     5041 gcacgccacg cgaggccgcc gaggggcgcc tgaacctgct ggtcggcggc gatgccgatc
     5101 tgctgcagga atgcctgccg ctgctgcaat gctttgccga gcacatcacc cacaccggcg
     5161 ccgcgggcag cggccaccgc atgaagctgc tgcacaacta tgtgtcgctg ggtagcgtgg
     5221 ccctgatcgc cgaggccgcc gcctgcgcgc tggcggccgg cgtggcgcca gaggtgttcg
     5281 tcgacgtgct ggccaagggc ggcggtggcg gcgtggcgct ggagcggctg cggccctatc
     5341 tgttggcgca ggatccgggt ggcctgcgtt tttccatgtc caacgcgctc aaggacatgg
     5401 gctactacag cgccatggca cgggacggtg aggcggcaca cggcatagcc caggccgtgc
     5461 atgacagctt tgccctggcc gtggccgatg gcgggccgca aagactggtg ccagagctgg
     5521 ttgaaatttt taagaaaaaa agccgctaac gcccgtacat aaagcgcgga cagctatcct
     5581 tttgttgtgc tgatacccag gcagtcggtc aggatggcgg tccattcccg tgccgctacc
     5641 ggcgtgatcg acagccggtt gccgcgctgc agcacgcgca tgtcggccag cgccgggtgc
     5701 gcgcgcagtt cggccaggcc gacgaggcgc gtcttgcgca gggcctgcac gtccagcagc
     5761 agccagcgcg gcttgcctgg ggcggccttg gggtcgtaat agggcgactg cgggtcgaac
     5821 tgcgtggggt cggcgcgcac gcccgaggcc acgcgcgcaa tgccggcaat gccgggttcg
     5881 gggcagctgg agtggtagaa cagtacgccg tcgcccactt gcatcgcgtc gcgcatgaag
     5941 ttgcgcgcct ggtagttgcg cacgccagtc caggggacgg tggcgccggc agccgccagg
     6001 gcgtcgtcga tggagcattc gtcaggctcc gacttcatca accagtagcg ggggggcttg
     6061 gtggtttcgg tagtcattca ggcaggccgc aatgcaaacc ggccgcgctg gggcggccgg
     6121 tggtagggca gggtgtgttc aacgggccga ggcagcgagg ttcgccgtct cctggtcgtt
     6181 tgccgcaacg cgacgcgcac cgttgaagcg gcgcgcccag taggccactc ccatgtcttc
     6241 gacgcgcacc tgcgcgcccg agcgcggggc atggatgaat ttgccatcgc ccacgtagat
     6301 gccgacatgg ctgaaggccc ggcgcatggt gttgaagaac accaagtcgc ccggttgcag
     6361 gtctttcttg tcgatgactt cggtggctgc ggcctgctcg ctggccttgc gtggcagcac
     6421 catgcccttg gcctggtcaa agatgacacg cacgaagccg ctgcagtcaa aaccggtttc
     6481 ggcgctgttg ccgccgcggc ggtagggcac gcctatgaag cccatggcgg tggagatcag
     6541 atcggaggtg cgatcggcga catcgtggcg caactcctgc agctgcccga tcaggccacg
     6601 ctccgccagc atgcgctcca cctcgtcggg aggagacgcg taagcattcg cgctcacaag
     6661 taacaaaacg ataagccaac gagacatgga gggggagggt aacacggcag tgggcaaatt
     6721 ccgccacaaa aatgttggaa tttgttgatc ctgctgaaat tccatgcatc gaggtcaagg
     6781 ccgatgctgg cctgtcgctg cctgcctacc cagattttct gttttcgtct gggctgttga
     6841 agttttgatt tattcttgcg tgctttttct ttttttcttc cagttccggt gtttgcttgc
     6901 tgctttggcg tgaatgccgc aagaaaagcc atcgcttgcc tgccgaactc tgcgatggat
     6961 tttgtttgat ttttgcgccc gctgcgaccg ggcttgattc ccatgacttt caaggacgcc
     7021 gcctcggggc agggggtgca gggtcagcat gacctgcagc gccatctcac caaccgccac
     7081 atccagctca tcgccattgg cggcgccata ggcacgggcc tgttcatggg ctcgggcaag
     7141 accatcagcc tggccgggcc ctccatcgtg ctggtctacg ccatcatcgg cgtgatggtg
     7201 ttctttgtga tgcgcgccat gggcgagctg ctcctgtcca acctgcgcta caagtcgttc
     7261 atagactttg cctccgacct gctcggcccc tgggcggggt tcttcaccgg ctggacctat
     7321 tggttctgct ggatcgtgac cggcattgcc gatgtgatcg cgatatcggc ctatgcgcag
     7381 ttctggtttc ctgacctgcc gcaatgggcg ccggcgctgg cctgcgtggg cctgctgctg
     7441 ggcctgaacc tgctcacggt gcggctcttt ggcgagatcg agttctggtt cgccatgatc
     7501 aagatcgtgg ccattgtcac gctggtgggc acgggcgtgg ccatggtcgc cggcggcttc
     7561 gtctcacccg caggccgcgc agccagcctg tccaacctgt ggaatgacgg cggcttgttt
     7621 ccgcatgggg ccatgggctt ttttgccggt ttccagatcg cggtgttcgc cttcgtgggc
     7681 atagagctgg tgggcaccac ggcggccgag gcgcaggatc ccgagcgcac gctgccgcgc
     7741 gccatcaact cgataccggt gcgcatcatc atcttctacg tctgcgcgct gattgccatc
     7801 atggccgtga caccctggcg cgacgtggtg ccgggcaaga gcccgtttgt cgagctgttc
     7861 gtgctggccg gcctgccggc cgccgccggg gtgatcaact tcgtggtgct gacctcggcc
     7921 gcttcgtcgg ccaacggcgg ggtgttttcc accagccgca tgctgtacgg gctggcgctc
     7981 aagggcgatg cgccgcgcgc gttctaccag ctctcgcgcg ccagcgtgcc ctcgcgcggc
     8041 ctgctgtttt cctgcggctg cctgctgttg ggggcgttct tgatgtgggt cgttcccgat
     8101 ctggtccagg ccttcacgct ggtcaccacg gtgtcggcca ttttgttcat gttcgtgtgg
     8161 tcgctgatcc tgctgtccta tatcgcctac cggcgccagc gccccgcgct gcatgccgcg
     8221 tccaaataca agatgcccgg cggtgtggcc atgtgctggg cctgcctggc gttctttgcc
     8281 ggcatcctgg tgctgctgtc cctggagacg gatacgcgcc aggcactggt ggtcacgccg
     8341 ctgtggtttg tgctgctggg cgtcgcctac cggttcatgc tgcggcgcca gccagagggt
     8401 gcgaccgtgg tgtcggtgcc gtcctgaggg caagacctgt ctgcacggga gtggtgacac
     8461 cgatggtctg atcggccagt tggcttgcta cggtttgtaa ggcgacgctg caattgccgc
     8521 tattctgtgc ggcgcaggtg caaccctgta ccgccattgt tgtcccaccc accatgttca
     8581 tactcgccgc gcgacgccga ctgctcgggc actggctgcc aggcctggcg ctggcgctgg
     8641 caggctgcgc ggccagctat gcgttgtggt tgcagcagac cgcgtccatg cagtccatcg
     8701 cgcaggcgcg ctttgcccag gagacacggt tgtttgccga tgcgctgcag cggcgcatgg
     8761 aatcgcatgc cgaattgctg cagggcatgc gcggcctgct gaccgtcaac ccggagctgc
     8821 ggcgcagcga gttcgagcgc gtggccagtg accttgcgtt ggccagcagc caccctgggg
     8881 tgaagaacat caacttcacc cgccatgttg ccggggatga gcgccgcgcc ttcgagacgc
     8941 gggcccgagg cgacgcccat ctggacggca gcctgccgac ggactttgcg attcggccgg
     9001 cgcaggagcg tccggagtat tacgtggtgg agtttctctg gccacgggct ggcaacgcag
     9061 gcattctggg gctggaaatc cattccgtgc cggaggcact ggaggccttg ctgcgcgccc
     9121 gcgacagcgc tggattgacc gcgtcggcgc catttcaatt ggcaggttcc gacggagacc
     9181 gaaccggcat catgctccgc ctgccggtgt ttgccggtcc agcgccgggt gatggccagg
     9241 cccggcgtat tctgggggcc gtcggtgtca gcgtgcatat cgagaccgcc atccacgcaa
     9301 tgcgcaatca ggggcagatg cacaacctgt cggtgaccct gtccgacatc ggcctggccg
     9361 gtggcgtgtc gccggcggcc gccgagcggc tgtaccaatc cccatccatg gacgcgtccg
     9421 ctgttgacgg catgcagcgc accgaggagg tgctggtggg cgggcggcgc tggttgctgc
     9481 agctacagcc gacggcgagc tttctgtcga gccaggagca gcggctgccg ggcctgctgg
     9541 ccctgggggg gctgctggtc acggcgttgc tcacagtctc cgtcagcatg ctggtgctgc
     9601 gccgcgcgcg cgcgctcagc gatgtgcggc aggccggcac cgctttaagc gagagcgagg
     9661 cacgcttcag cgccgtgttc catcaggccg ccgtgggctt tgtgcaggtg gatctggaca
     9721 gcgacaggct ggtgcgcatg aaccagaagt tttgcgacct gctgggctat accgagcagg
     9781 agctgcagca gctgcgcttc caggacatca ccgtggccga ggatctgcag gacgatctgg
     9841 cgcaaatggc gcgcctgcgc gccggcgagc tgcgcgactt ccggatggag aagcgctacc
     9901 ggcgcaagga tggtggcgtc gtatgggccg agctgtcggt gtcacgcctg cgcctggccg
     9961 atggcactgg ctaccacctg gccgtggtgc aggacatcag cgagcgcaag cgcatggagc
    10021 aggccctgcg cggcagcgag cagcgcttgc tcggcatcct gaaccacatg cccgtgggcg
    10081 tgaacctggt gcaggacgag cgcatcgtgt tccgcaatgc cagccatgtg cagatctgcg
    10141 gctatggcgc cgacgaagcg cccgatgtgg acagtctgtg gagcctgacc atccccgacg
    10201 ccgcgcagcg cgagcaggtg cggcgctcct ggcaggccgc ctgcgcagcg gcgcgccggg
    10261 ccgcagtgga tggcggcgcc gccatcgcgc cgctggaatg cgtgatcacc gccaagtccg
    10321 gcgtgcagca catcgtcgag ctgtccggca tgatgctgga ggacagccac atcgtcacca
    10381 tggtggattt gagccagcgc cgccgggccg agcaggaggt gcgctatctg gcctacaacg
    10441 acccgctgac cggcctgccc aaccggcgcc tgctgcttga ccgcctgcag caggcgctgg
    10501 ccatcagcgc gcgccaccaa tggtgcggcg cgctgttgat gctggacctg gacaacttca
    10561 agcagatcaa cgagaccctg ggccacgaca tgggcgaccg actgctgcag gccgtggccg
    10621 agcgcctgcg cgcctgtgtg ccggacgacg acacgctggc gcgccatggc ggcgacgagt
    10681 tcgtggtggt gctcaaggat ctgggcgcca cgccggcgga ggccgcagcc agggccgagg
    10741 aggccgcgca gaagatgctg caggccatgc gcgcgccttt cgtgatcgat ggcggcgagc
    10801 cgcgccactc cacgctgagc gtgggcatcg ccgtcttcca gggccagcgc gagtcggccg
    10861 atgaactgct caagcgcagc gacatggcca tgtacgaggc caaggcggcc gggcgcgacg
    10921 cactgcgctt cttcgacccg cagatgcagg cccagatggc cgagcgcgtg gcgctggagg
    10981 ccgacatgcg cgccgggctg gacagtggcc agttcgaact gttctaccag ccgaagatgg
    11041 tgcgcggcca gatcacgggc gccgaggcgc tgctgcgctg gcgccaccct gtcaagggct
    11101 acgtgtcccc ggccgaattc attccgctgg ccgagcaatg cggcctgatc ctgcgcctgg
    11161 gccaatgggt gttgcgcacg gcctgcgagc ggctggcgca ctggcaggag cacccagtgc
    11221 tgggccagct gacggtggcg gtgaacgtca gcccgcgcca gttctacgag gccggcttcg
    11281 taccccaggt gctggaggca ttggccggag ccggcgccga tgcgcggcgc ctgcgcctgg
    11341 agctcaccga gggcctgctg ctgcaggacg tggaggacac cattgccaag atggtgcagc
    11401 tgcgcggcta tggcgtgggc ttttcgctgg acgactttgg caccggctat tcatcgctgg
    11461 cctacctgaa gcgcctgccg ctgcacgagc tgaagatcga ccagagcttt gtgcgcgacg
    11521 tgctcaccga ccccaacgac gcagccatcg cccgcaccat cgtcgccctg ggcaccagcc
    11581 tgggcctgca ggtgacggcc gaaggggtgg agaccgaggc gcagcgcctg ttcctggagc
    11641 gcgcaggctg tcatgcctgg cagggttact tgctcagccg cccggtgccg ggtctggcat
    11701 tcgaggacct ggtgctggca catgcagccg ccgtcggccc ccatgtccca tagtgccgcc
    11761 agcgcagaag cctgcgcagt cccgtcatgc aggtagtcac caccctggcc ctgttcctgg
    11821 ccacggcgct ggccgagatc ctcggctgct atctgccctg gctgtggctg cgccaggggc
    11881 gcagcgcctg gctgctgctg ccggccgccg ccagcctggc cctgtttgcc tggctgctga
    11941 cgttgcaccc ggccgccgcc gggcgtgtgt atgcggccta cgggggcgtc tatgtggccg
    12001 tggcattggc ctggctgtgg gccgtggacg gcatacgccc cggcccctgg gactggctgg
    12061 gcgtggccgt cacgctgtgc ggcatggcca tcattgcctt tgcgccgcgc ggctgacggc
    12121 agtcacggat gcataattga tagctgcatg ccattgttgt acaggcgttt caggacattt
    12181 ttgctaaaaa aacaaaagcg ctcgatgagc gctttttgag gacgacagaa ctgcttagag
    12241 tt
//
LOCUS       DAOMTQ010000091        12198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_942_length_12198_cov_27.222597, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000091 DAOMTQ010000000
VERSION     DAOMTQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_12198_cov_27.222597"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1339
                     /gene="ftsA"
                     /locus_tag="PK518_09830"
     CDS             110..1339
                     /gene="ftsA"
                     /locus_tag="PK518_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRM49256.1"
                     /translation="MAREYKDVVVGLDIGTAKVMAVVAEVLPGGELKLAGLGVAPSNG
                     LKRGVVVNIDATVQSIQQALKEAELMADCKIQRVYTGITGSHIRGLNSSGMVAVKDKE
                     VTPADVARVVETAKAINISSDQRLLLVEPQEFIIDGQDVREPIGMSGMRLEAKIHIVT
                     GAQSAAENIIKCVRRCGLEVEQLMLNPLASSQAVLTDDERELGVAVVDIGAGTTDVAI
                     FTGGAIRHTAVLPIAGDLITSDIAMALRTPTKDAEDIKVESGFAKQLLADPEAQVEVP
                     GLGDRSPRMLSKQALAGVIEPRVEEIFSLVQQVIRESGYEEVLSSGIVLTGGSCVMPG
                     MVELGEDIFLKPVRRGLPKYSSSLADMIAQPRAATVMGLLEEARLARLRGFKVAAKSG
                     SVKTAFGRFKDFIVGNF"
     gene            1502..2731
                     /gene="ftsZ"
                     /locus_tag="PK518_09835"
     CDS             1502..2731
                     /gene="ftsZ"
                     /locus_tag="PK518_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRM49257.1"
                     /translation="MTIEMIESEEFNQGTQIKVIGVGGGGGNAVQHMISRSVQGVEFI
                     SANTDAQALTRSTAHRTIQLGTNGLGAGSKPEKAREAAEAAEEDIRQAIAGAHMLFIT
                     AGMGGGTGTGAAPVIAKVAKEMGILTVGVVTKPFDWEGGRRMKNADEGLAELEANVDS
                     LIVVLNEKLLDVLGDDITQDEAFAHANDVLKNAVGGIAEIINEYGQVNVDFEDVRTVM
                     GEPGKAMMGTATANGPDRARIAAEQAVACPLLEGIDLSGAKGVLVLVTASKGSLKLSE
                     SRLAMNTINAYASPDAHVIFGAAYDDSLGDDIRVTVVATGLSRANARRQTMQVVQGSL
                     RTGTDNVAYQMPMAGGALGVPGGIIGSGAQAEYGNMQVPSVWRTNRTAATARVDALSS
                     GGMDELEIPAFLRKQAD"
     gene            2863..3786
                     /gene="lpxC"
                     /locus_tag="PK518_09840"
     CDS             2863..3786
                     /gene="lpxC"
                     /locus_tag="PK518_09840"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRM49258.1"
                     /translation="MLQQRTIKTLTRAVGVGLHSGQRVELTLRPAQADTGIVFRRVDL
                     PEPVDIAINAQAVVDTRMASTIGAGGAKVHTVEHLMSACAGLGLDNLYIDITAEEVPI
                     LDGSSASFVFLLQSAGVELQNAPKRFIRVKRAVEVREGQGDQLKWARLEPYHGFKLRF
                     EIDFAHPAVDSTGQCVEFDLGSGNYARDIARARTFGFTKDVEMLRSSGLALGGGLDNA
                     IVMDDYKVLNADGLRYDAEFARHKILDAMGDLYLVGKPLLAAYSAFRSGHGMNNQLLR
                     ALLAQEDAWEIVSFDSERQAPAGFAQPARAW"
     gene            3797..3970
                     /locus_tag="PK518_09845"
     CDS             3797..3970
                     /locus_tag="PK518_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49259.1"
                     /translation="MLIMRWAVMLLLLAAGVAFAYFAATGQQRYKRFGLVIIKWIAIA
                     AAMFFLVLILERI"
     regulatory      3997..4106
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4185..6059
                     /gene="thiC"
                     /locus_tag="PK518_09850"
     CDS             4185..6059
                     /gene="thiC"
                     /locus_tag="PK518_09850"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRM49260.1"
                     /translation="MNAPDSFATLLARTRESFPASTKSFITGSRADLRVPVRDIALTN
                     GEVVSVYDTSGPYTDPAAIIDVKQGLQSVRGAWIAERGDTERYEGRKPVALDDGSKSE
                     DAARLAQLRQEAAALQRQPLRAKAGANVTQMHYAKKGIITPEMEYVALRENGKREWMA
                     QYMADAAREKRLAGNPMGALIPKIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRN
                     FLVKINANIGNSAVTSSIEEEVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPV
                     PIGTVPIYQALEKVGGIAEDLSWEIFRDTLIEQAEQGVDYFTIHAGVRLAYIHLTAQR
                     RTGIVSRGGSIMAKWCMAHHRESFLYEHFEDICDIMKAYDVSFSLGDGLRPGCASDAN
                     DEAQFAELHTLGELTQIAWKHDVQTMIEGPGHVPMHMIQANMTEQLKTCHEAPFYTLG
                     PLTIDIAPGYDHIASAIGAAMIGWMGTAMLCYVTPKEHLGLPDRDDVKQGIIAYKIAA
                     HAADVAKGHPGARARDDALSQARFDFRWQDQFNLGLDPETAQEYHDETLPKDAAKVAH
                     FCSMCGPKFCSMKITQEVREFAAAKGVAEGDALAQGMAQKAEEFNRAGGEFYIPIQPV
                     "
     gene            complement(6289..6609)
                     /locus_tag="PK518_09855"
     CDS             complement(6289..6609)
                     /locus_tag="PK518_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HRM49261.1"
                     /translation="MALRPARSHRRAVHPDQIAKQSAPKFRKLGSIGLISKDYVRNEH
                     GAGGLYVWESRAAAEAWFTEAKLQEYTTLFGARPTLTWYEANLTVDNKAGQTRVNGQP
                     VAEG"
     gene            complement(6734..7282)
                     /gene="ruvC"
                     /locus_tag="PK518_09860"
     CDS             complement(6734..7282)
                     /gene="ruvC"
                     /locus_tag="PK518_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRM49262.1"
                     /translation="MRILGIDPGLQTTGFGVVDVEGQRLAYVASGTIRTTGLALGDLP
                     GRLKLLFDGITEVAARYRPDAAAVEIVFVNVNPQSTLLLGQARGAALTALVNADLPVA
                     EYTALQMKKAVVGHGRAAKSQVQEMVRRLLTLPGLPGTDAADALGLAITHAHAGTAMA
                     RVGQVTQLSRRQHAMYKGGRSY"
     gene            complement(7324..8166)
                     /locus_tag="PK518_09865"
     CDS             complement(7324..8166)
                     /locus_tag="PK518_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49263.1"
                     /translation="MPLAIETRHGGARLRNWVAALAVALPPALLLGALLAAILPAPWR
                     NAAVYASLLFVPCLWAGARIGPTLIARYDDQALQPPPAAEPGLRQAPTLDAAQQRAWA
                     ALESWCQAGTGDGRKPFWHPWRLPDIPERLGLAVMAGRDCDRASHLAAAFARHLDRDD
                     ELAALSAASRLKGWQLKLAVKWHELWWWRRRHPRQPWDCGYLAPDPAAPDRLARFRPR
                     RPTLIIASTLRGDSLAQTLQTLAAAKAEYHHPVRLLMLDADAQPWPARVYGPGQVTLI
                     QFAS"
     gene            complement(8181..8897)
                     /gene="mtgA"
                     /locus_tag="PK518_09870"
     CDS             complement(8181..8897)
                     /gene="mtgA"
                     /locus_tag="PK518_09870"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRM49264.1"
                     /translation="MRGVLRWIALVLLAFVALQLFFVARIAAMAWLDPESTSFQRSEA
                     WAQLRQDGGLHWRQQWLPYAQISDHLKRAVIASEDDNFVNHDGVEWDAIEKAWERNAR
                     AEAQAAARPNARAPKIRGGSTITQQLAKNLLLSGERNLVRKGQEFMLTLALERLLSKQ
                     RILEIYLNNVEWGAGIFGAEAAAQHYYRKSARQLTAPEAARLAVMLPAPKRFEKTPNS
                     AYLAGRTRTIMARMASAELP"
     gene            complement(9069..9911)
                     /gene="aroE"
                     /locus_tag="PK518_09875"
     CDS             complement(9069..9911)
                     /gene="aroE"
                     /locus_tag="PK518_09875"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRM49265.1"
                     /translation="MSTTPDLYCVMGNPIAHSRSPWIHARFAELTGQQLHYERRLVAL
                     DGFAQALRDFAAAGGRGCNITVPFKLQAADLADTRSARVQLAGAANTLVFANGQIHAD
                     NTDGLGLVADIERNAGVALAGRDLLLVGAGGAAAGALAPLIERQPRRIAVVNRTSARA
                     QALVASHAALASLHKTELLALDIQAPRADFDVIINATASSLAGGAVPVPASVLRAGSL
                     AYDMMYGPAAQGFLDWATQHGAQARDGLGMLVEQAAEAFQLWRGQRPPAAQVLQELRA
                     AIAK"
     gene            complement(10167..11027)
                     /locus_tag="PK518_09880"
     CDS             complement(10167..11027)
                     /locus_tag="PK518_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRM49266.1"
                     /translation="MKVPPFLLRLSTLQIALGVSIALHALLLTVRFIDPERFNRVFED
                     TPLEVILVNAKTSERPDKAQAIAQASLAGGGEAAKGRATSPLPYSALTSIGDDLEEAQ
                     RKMDQMLEQQTQLLAQVRQQLAILPQPDPRKASESTAQEQQEERRRQLVKLLAEIEKR
                     INDENARPRKRYISPATREEAYAIYYDGLRRKVEDKGTQNFPEQGGKKLYGELIMILT
                     VNHDGRVLETEVVQGSGNRLLDRRAEAIARAAGPFGHFGPAMRAKADQIAVVSRFKFT
                     HDQTLETAVR"
     gene            complement(11120..>12198)
                     /locus_tag="PK518_09885"
     CDS             complement(11120..>12198)
                     /locus_tag="PK518_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRM49267.1"
                     /translation="EATLAITASETRLERVPVTVNLRHDQLDHIVTEQWLNDASIQHE
                     DTPQRLLELREQLSFLYKLAKQLKAGREQVRGKPETFNRPDYNFRLEGNNKAEPQGSE
                     TVRISTRQRGAPLDLIVAEAAIVANSHWGQMLAEHGVPGIYRSQASLAPGIKVRMGTK
                     ALPHAGIGVKSYAWATSPLRRYVDLVNQWQIIACARHGKTAALAAPFKPKDAELFSII
                     SSFDATYGAYNGYQAGMERFWTLKYLEQNQITELDATVIKEGMNGGFLVRADSLPLVL
                     PVLGAQGLPRGARVRVKLGEIDEITLDVNGTLIARLDDPLDASDDGPVDEDEEDTVAG
                     PIAIAVDINETDGAGSEPGTGQPG"
BASE COUNT         2010 a         4000 c         4234 g         1954 t
ORIGIN      
        1 ggccgagcgt gcggcggcag cggcggcgcg tgccgcgcaa gcggcgcagc gggcagaggc
       61 caaggcgcgt gcggcggcaa agaacaactg agggcaggac gaaagaacta tggcaagaga
      121 gtacaaagac gtggtcgtgg ggctggacat cggcaccgcc aaggtcatgg ccgtggtggc
      181 cgaggtgttg cccggtggcg agctcaagct cgccggtcta ggtgtggcgc cttccaatgg
      241 cttgaagcgc ggcgtggtgg tgaacatcga cgccacggtg cagagcatcc agcaggcgct
      301 gaaggaggcc gagctgatgg ccgactgcaa gatccagcgc gtctacaccg gcatcaccgg
      361 cagccatatc cgcgggctca actccagcgg catggtggcg gtgaaggaca aggaggtcac
      421 gccggccgac gtggcgcgcg tggtcgagac ggccaaggcc atcaacatct ccagcgacca
      481 gcgcctgctg ctggtcgagc cgcaggagtt catcatcgac ggccaggacg tgcgcgagcc
      541 catcggcatg agcggcatgc gcctggaggc caagatccac atcgtcaccg gcgcgcagag
      601 cgcggccgag aacatcatca aatgcgtgcg ccgctgcggc ctggaggtcg agcagctgat
      661 gctcaacccc ctggccagca gccaggccgt gctgaccgat gatgaacgcg agctgggcgt
      721 ggccgtggtc gatatcggcg ccggcaccac cgacgtggcc atcttcaccg gcggcgccat
      781 tcgccacacg gccgtgctgc cgattgctgg cgacctgatc accagcgaca tcgccatggc
      841 gctgcgcaca ccgaccaagg acgccgagga catcaaggtg gagagcggct ttgccaagca
      901 gctgctggcc gaccccgagg cccaggtgga ggtgccgggc ctgggcgacc gcagcccgcg
      961 catgctcagc aagcaggcgc tggccggcgt catcgagccg cgcgtggagg agattttctc
     1021 gctggtgcag caggtgatac gcgagtcggg ctacgaggaa gtgctgtcgt cgggcatcgt
     1081 gctcaccggc ggcagctgcg tcatgcccgg catggtggag ctgggcgagg acatcttttt
     1141 gaagcccgtg cgccgcggcc tgccgaagta ttccagctcg ctggccgaca tgattgccca
     1201 gccgcgcgcg gccaccgtca tgggcctgtt ggaagaagcg cgcctggcgc gcctgcgcgg
     1261 cttcaaggtg gcggccaaga gcggctcggt gaagacggcg tttggccgct tcaaggactt
     1321 catcgtgggg aacttctgac atgacggccg cattgctctg gctggcccgc gccgggccgc
     1381 cgcgtcgaaa gcggggccga tggcgaagta cggccccgcc gcctgacggc atcacgcgtc
     1441 acaacaacaa gcatcgattt ttggcaattg cacatcacaa cgtagttaca ggagcagcat
     1501 catgaccatc gaaatgatcg agtccgaaga gttcaaccag ggcacccaga tcaaggtgat
     1561 cggtgtcggc ggcggcggcg gcaacgccgt gcagcacatg atctcgcgca gcgtgcaggg
     1621 cgtggagttc atcagcgcca acacggatgc acaggcgctg acgcgcagca ccgcgcaccg
     1681 caccatccag ctgggcacca acggcctggg cgcgggcagc aagcccgaga aggcacgcga
     1741 ggccgccgag gccgccgagg aagatatccg ccaggcgatt gccggcgcgc acatgctgtt
     1801 catcaccgcc ggcatgggcg gcggcaccgg cacgggcgcg gcgccggtga tcgccaaggt
     1861 ggccaaggag atgggcattc tcaccgtcgg cgtggtcacc aagcccttcg actgggaggg
     1921 tggccgccgc atgaagaatg ccgacgaagg cctggccgaa ctggaggcga acgtcgattc
     1981 gctgatcgtg gtgctgaacg aaaagctgct cgacgtgctg ggcgacgaca tcacccagga
     2041 cgaggccttt gcccacgcca acgatgtgct gaagaacgcc gtcggcggca tcgccgaaat
     2101 catcaacgag tacggccagg tcaacgtgga ctttgaagac gtgcgcaccg tgatgggtga
     2161 gcccggcaag gccatgatgg gcacggccac cgccaacggc ccggatcgcg cccgcatcgc
     2221 cgccgagcag gccgtggcct gcccgctgct ggagggcata gacctgtctg gcgccaaggg
     2281 cgtgctggtg ctggtgacgg ccagcaaggg cagcctgaag ctgtccgaat cgcgcctggc
     2341 gatgaacacc atcaacgcct acgcctcgcc cgatgcgcat gtcatcttcg gcgccgcgta
     2401 tgacgacagc ctgggcgacg atatccgcgt caccgtggtc gccaccggcc tgtcgcgcgc
     2461 caacgcgcgc cgccagacca tgcaggtggt gcagggcagc ctgcgcaccg gcaccgacaa
     2521 cgtggcctac cagatgccca tggccggcgg cgccctgggc gtgcccggcg gcatcatcgg
     2581 cagcggcgcc caggccgagt acggcaacat gcaggtgccc agcgtctggc gcaccaaccg
     2641 cacggccgcc acggcacgcg tggacgcgct gtcctcgggt ggcatggatg agctggaaat
     2701 cccggccttc ctgcgcaagc aggctgattg acacaggtca gcccaggccg gcggctggct
     2761 cttttggggg cctgcctgcc ggctttttca tttcccgttg gctatcagcg gcatagccaa
     2821 ctgccgcctg tcgggcgcgg cagcgccaac taaaatcgca ccatgctcca gcaacgcacc
     2881 atcaagaccc tgacccgtgc cgtcggcgtg ggtctgcaca gcggccagcg ggtcgagctg
     2941 acgttgcgcc cggctcaagc agacacgggc atcgtgttcc gccgcgtcga tctgcccgag
     3001 ccggtggaca ttgccatcaa cgcccaggcg gtggtggata cgcgcatggc ctccaccatc
     3061 ggcgcgggcg gggccaaggt gcataccgtg gagcatttga tgtcggcctg cgccggcctg
     3121 gggctggaca acctgtacat cgacatcacg gccgaggagg tgcccatcct cgatggctcc
     3181 tcggcctcgt tcgtgttcct gctgcaaagc gccggcgtgg agctgcagaa cgcgcccaag
     3241 cgcttcatcc gcgtcaagcg cgcggtggag gtgcgcgagg gccagggcga ccagctgaaa
     3301 tgggctcgcc tggagcccta ccacggcttc aagctgcgct tcgagatcga ctttgcccat
     3361 ccggcggtcg attccaccgg gcagtgcgtg gagtttgatc tgggcagcgg caactacgcc
     3421 cgcgacatcg cgcgcgcgcg caccttcggc ttcaccaagg acgtggagat gctgcgctcc
     3481 agtggcctgg cgctgggcgg tgggctggac aacgccatcg tcatggacga ctacaaggtg
     3541 ctgaacgccg acggcctgcg ctacgacgcc gagttcgccc ggcacaagat cctggacgca
     3601 atgggcgacc tgtacctggt gggtaagccg ctgctggcgg cctacagcgc gtttcgcagc
     3661 ggccatggca tgaacaatca gctgctgcgc gcgctgctgg cgcaggaaga cgcctgggag
     3721 atcgtcagct ttgacagcga gcgccaggca cccgcgggct ttgcccagcc ggcgcgcgcc
     3781 tggtgagtcg ccggccatgc tgatcatgcg ctgggccgtg atgctgctgc tgctcgcggc
     3841 tggcgtcgca ttcgcttatt tcgccgccac gggccagcag cgctacaagc gcttcgggct
     3901 ggtgatcatc aagtggatcg ccatcgcggc ggccatgttc ttcctggtgt tgatactgga
     3961 acgcatatag tgcgcggcgc gccaggccgt atactgcgcc ttgctccggg gtgcacgttt
     4021 gggcgtgctg agatggtggg ccaacccgcc aaaaccgcga acttgatctg gttagtacca
     4081 gcgtaagaag agctgcagtg ggatggggga gtgcgccgta gagcgcgcct tcgtcccgcc
     4141 gattgcggag cattccatcc cccagccttt gaggaatgcc cgccatgaac gcccctgaca
     4201 gcttcgccac cttgctcgcc cgcacgcgcg agtcctttcc cgcctccacc aagtccttca
     4261 tcaccggcag ccgcgcggat ctgcgtgtgc cggtgcgcga cattgccctc accaacggcg
     4321 aggtcgtcag cgtctatgac acctccggcc cctacaccga ccccgccgcc atcatcgacg
     4381 tgaagcaggg cctgcaaagc gtgcgcggcg cctggattgc cgagcgcggc gacaccgagc
     4441 ggtatgaggg ccgcaaaccc gtggcgctgg acgatggcag caagagcgag gacgccgccc
     4501 gcctggccca gctgcgccaa gaggccgcgg ccctgcaacg ccagcccctg cgcgccaagg
     4561 ccggcgccaa cgtcacgcag atgcactacg cgaagaaggg catcatcacc cccgagatgg
     4621 aatacgtggc gctgcgcgaa aacggcaagc gcgagtggat ggcgcagtac atggcagacg
     4681 ccgcgcgcga aaagcgcctg gccggcaacc ccatgggcgc gctgatcccg aagatcatca
     4741 cccccgaatt cgtgcgcgac gaggtggcgc ggggccgcgc catcatcccg gccaacatca
     4801 accaccccga ggtggagccg atggccattg gccgcaactt cctcgtcaag atcaacgcca
     4861 acatcggcaa ttccgccgtc acgtccagca tcgaggaaga ggtggaaaaa ctggtctggg
     4921 ccattcgctg gggcgcagac aacgtgatgg atctctcgac cggcaagaac atccacacca
     4981 cgcgcgactg gatcgtgcgc aacagtcccg tgcccatcgg caccgtgccc atctaccagg
     5041 cgctggaaaa ggtgggcggc atcgccgaag acctgagctg ggaaatcttc cgcgacacgc
     5101 tgatcgagca ggccgagcag ggcgtggact acttcaccat ccacgcgggc gtgcgcctgg
     5161 cctatatcca cctgacggcg cagcggcgca ccggcatcgt ctcgcgcggc ggctccatca
     5221 tggccaagtg gtgcatggcg caccaccgcg agagcttcct gtacgagcac ttcgaggaca
     5281 tctgcgacat catgaaggcg tacgacgtgt cgttcagcct gggcgacggc ctgcgccccg
     5341 gctgcgccag cgacgccaat gacgaggcgc agtttgccga gctgcacacc ctgggcgagc
     5401 tgacgcaaat cgcctggaag cacgacgtgc agaccatgat cgaaggcccc ggccatgtgc
     5461 ccatgcacat gatccaggcc aacatgaccg agcagctcaa gacctgccac gaggcgccgt
     5521 tctacaccct gggcccgctg accatcgaca tcgcccccgg ctacgaccac attgccagcg
     5581 ccattggcgc ggccatgatc ggctggatgg gcacggccat gctgtgctac gtgacgccca
     5641 aggagcacct gggcctgccc gaccgcgacg acgtcaagca gggcatcatc gcctacaaga
     5701 tcgccgcgca tgccgccgac gttgccaagg gccacccggg ggcgcgcgcg cgtgacgatg
     5761 cgcttagcca ggcgcgcttc gacttccgct ggcaagacca gttcaacctg ggcctggacc
     5821 ctgaaaccgc gcaggaatac cacgacgaaa cattaccgaa ggacgccgcc aaggtggcgc
     5881 atttctgcag catgtgcggg cccaagttct gctccatgaa gatcacgcaa gaggtgcgcg
     5941 aatttgccgc cgccaagggc gtggccgagg gcgacgcgct ggcccagggc atggcgcaaa
     6001 aggccgagga attcaaccgc gcgggcgggg agttctacat tcctatccag ccggtgtgat
     6061 accgacactt cccctctggg tatgggtgcc tacatatttc agtagcgcaa aacgctgatt
     6121 tgacgcgggt ctcaactccc tctccccctt gggggagagg gtaggggtga gggggtgatg
     6181 ggtcgcgacg ccggcatggg ccaccctcac ccctaccctc tcccgccagc gggagaggga
     6241 gaagcagcct cgaggctgcg cacatcctca ggggggaggg agagctcgtc aaccctcggc
     6301 caccggctgg ccgttgaccc gcgtctgccc ggccttgttg tccaccgtca ggttggcctc
     6361 gtaccaggtc agcgtggggc gcgcgccgaa cagggtggtg tactcctgca gcttggcctc
     6421 ggtaaaccag gcctcggctg cggcgcggct ttcccatacg tacaggccgc cggcgccatg
     6481 ctcgttgcgc acgtagtcct tgctgatcag gcctattgag ccgagcttgc ggaacttggg
     6541 cgccgactgt ttggcgatct gatctggatg aactgctctt cggtgggagc gggcagggcg
     6601 aagtgccaca ggacgacgca gggggccatg gcgtgggact ccaaaaaggc taaggggaaa
     6661 tcattgtagg cagcgccagc actccccgga caagggccca agccgccagg cgaaggtggc
     6721 aactgacgcg gtttcagtaa ctgcgcccgc ccttgtacat ggcgtgctgg cgccgcgaga
     6781 gttgcgtcac ctgccccacg cgcgccatgg ccgtgccggc gtgggcatgg gtaatggcca
     6841 gccccagcgc atcggccgcg tcggtgcccg gcaggccggg cagggtgagc aggcggcgca
     6901 ccatctcctg cacctgcgac ttcgccgcgc ggccgtggcc gaccacggcc ttcttcatct
     6961 gcagggccgt gtattcggcc acgggcaggt cggcgttgac cagcgcagtg agcgccgcgc
     7021 cgcgcgcctg gcccagcagc agggtggact gggggttgac gttgacgaac acgatctcca
     7081 ccgccgccgc atcgggccgg tagcgcgcgg cgacctcggt gatgccgtcg aacagcagct
     7141 tgagccgccc cggcaggtcg cccagcgcga ggcccgtggt gcgtatggtg ccgctggcca
     7201 cgtaggccag gcgctggccc tcgacgtcca ccacgccaaa gccggtggtc tgcaggccgg
     7261 ggtcgatgcc gagaattctc attgctatta aaaatagagc tgttggcgct ttatgtacaa
     7321 gcgtcaggag gcaaattgaa tcaaagtaac ctgccctggg ccatagaccc gcgcaggcca
     7381 aggttgggca tcggcgtcga gcatgagcag tcggacaggg tggtggtatt ccgcctttgc
     7441 cgcagccaga gtctgcagcg tctgcgccag actgtcgccc cgcagcgtgc tggcgatgat
     7501 cagcgtaggc cggcgcggcc tgaagcgtgc cagccgatct ggtgccgcgg ggtctggcgc
     7561 gaggtagccg cagtcccagg gctggcgcgg gtgccgcctg cgccaccacc atagctcgtg
     7621 ccacttcacg gcgagtttga gctgccagcc cttgagcctt gaagccgcgg acagcgcggc
     7681 gagttcatcg tctcggtcga gatgccgcgc aaaggccgcg gccaggtggg atgcgcgatc
     7741 acagtcccgt cccgccatga cggccaggcc gaggcgctcg ggtatatcgg gcagccgcca
     7801 ggggtgccag aacggcttgc gcccgtcccc tgtgcctgcc tggcaccagg attcaagggc
     7861 cgcccacgcg cgctgctgtg ccgcatccag cgttggtgcc tgccgcaatc ccggttccgc
     7921 ggcagggggc ggctgcagcg cttggtcgtc ataacgggcg atcagggtgg ggccgatgcg
     7981 tgcgcccgcc caaagacatg gaacgaaaag cagggatgcg tacacggccg cattgcgcca
     8041 aggggcgggc aatatggcgg ccagtagcgc gcccagcagc agcgcgggcg gcagcgccac
     8101 ggcgagggcg gccacccagt tgcgcaggcg agcgccgccg tggcgggttt cgatggccag
     8161 gggcatcttt ggacccgtgc tcatggcaat tcggcgcttg ccatgcgcgc catgatggtg
     8221 cgcgtgcgcc ccgccagata cgccgaattc ggcgtttttt caaagcgctt gggtgccggc
     8281 agcatcacgg ccaggcgggc ggcctcgggg gcggtgagct ggcgcgcgct ctttcggtag
     8341 taatgctggg cggcggcctc ggcgccgaag atgccggcgc cccattccac gttgttcagg
     8401 tagatctcca ggatgcgctg cttgcttaac agccgctcca gcgccagcgt gagcatgaat
     8461 tcctggccct tgcgcaccag gttgcgttcg cccgagagca gcaggttctt ggccagctgc
     8521 tgggtgatgg tggagccgcc acggattttg ggtgcgcggg cgttgggccg ggcggcggcc
     8581 tgggcctcgg cgcgggcgtt gcgctcccag gccttttcta tggcgtccca ttccacaccg
     8641 tcgtggttga cgaagttgtc gtcctcggag gcgatcacgg cgcgcttcag gtggtcggag
     8701 atctgcgcgt agggcagcca ttgctggcgc cagtgcaggc cgccgtcctg gcgcagctgc
     8761 gcccaggcct cggagcgctg gaagctggtg gattcggggt caagccaggc catggcggcg
     8821 atgcgcgcca cgaagaacag ctgcagcgcc acaaaggcca gcagcaccag ggcgatccag
     8881 cgcagcacgc cccgcatttc agtaatccac ggcgctgcgc agtacccgct gcccatgcaa
     8941 ctggcgcaac ccaggctggg gcagccgagc aagggccgcc ccgcagcgag ggctgcgtcc
     9001 cccttcccgc agcgcgcagc ggtgcgagag aagggggaag cggcgcagcc gcacaggggg
     9061 ttgttgcttc attttgcgat ggccgcgcgc agctcttgca gcacctgggc ggcgggcggg
     9121 cgctggccgc gccagagctg gaaggcctcg gccgcctgtt ccaccagcat tcccaggccg
     9181 tcgcgcgcct gggcgccgtg ctgggtcgcc cagtccagaa agccctgggc ggcggggccg
     9241 tacatcatgt cgtaggccag gctgccggcg cgcagcacgc tggcgggcac gggcacggcg
     9301 ccgccggcca ggctgctggc cgtggcgttg atgatgacat caaaatcggc cctcggcgct
     9361 tgtatatcaa gcgctagaag ctctgtttta tgtagcgatg ccagcgccgc gtggctggcc
     9421 accagggcct gggcgcgcgc gctcgtgcgg ttcaccacgg cgatgcgccg gggctggcgt
     9481 tcgatcagcg gtgccagcgc gccggcggcg gcgccgcccg cgccgaccag cagcaggtcg
     9541 cgcccggcca gcgccacgcc ggcattgcgc tctatgtcgg ccaccaggcc caggccatcg
     9601 gtgttgtcgg cgtggatctg gccgttggca aagaccaggg tgttggccgc gccggccagt
     9661 tgcacgcgcg cgctgcgcgt gtcggcaagg tctgcggcct gcagcttgaa gggcacggtg
     9721 atgttgcagc cgcgcccgcc ggcggcggca aagtcgcgca gcgcctgggc aaagccgtcc
     9781 agcgccacca ggcggcgttc gtagtgcagc tgctggcccg tcagctcggc aaagcgcgcg
     9841 tgtatccagg gcgagcggct gtgggcgatg gggttgccca tcacgcagta caggtcgggg
     9901 gtggtgctca tcggtattag tttctcatcg cactttgcgt aacccgctac gcatgaaact
     9961 ggcgccaccc aagccagggc cgccgcgcaa gggccgcccc gccgcgctgg cggcgtcccc
    10021 ctgcccgcat tgcgcagcaa tgcgagagcg ggggctgagg gccgcggctc caagcctgcc
    10081 tgcgcaggct tggacggccc cgaagggctg ccgcctctgg cggctgcacg gagcggcgta
    10141 gccgcacagg ggggtgttgt ttcactttag cgcacggcgg tttccagggt ctggtcgtgc
    10201 gtgaacttga agcgcgagac cacggcgatc tggtcggcct tggcgcgcat ggccgggccg
    10261 aagtgaccaa acggcccggc tgcgcgggca atggcctcgg cgcggcgatc gagcaggcga
    10321 ttgcccgagc cctgcacgac ctcggtctcc agcacgcgac cgtcgtggtt cacggtcagg
    10381 atcatgatca gctcgccgta gagcttcttg ccgccctgct cgggaaagtt ctgcgtgccc
    10441 ttgtcctcca ccttgcggcg cagcccgtcg tagtagatgg cgtaggcctc ctcgcgcgtg
    10501 gccgggctga tgtagcgctt gcgcgggcgg gcgttttcgt cgttgatgcg cttttctatc
    10561 tcggccagca gcttgaccag ctggcggcgc cgttcttcct gttgttcctg tgcggtgctt
    10621 tcgctggcct tgcgcgggtc gggctggggc aatatggcca gctgctggcg cacctgggcc
    10681 agcagctggg tttgctgctc cagcatctga tccatcttgc gctgggcttc ctccaggtcg
    10741 tcgccaatgg aggtcagggc cgagtagggc agggggctgg tggcgcggcc cttggccgcc
    10801 tcgcccccgc cggcgagcga ggcctgggcg atggcctggg ccttgtccgg gcgttcgctg
    10861 gtcttggcgt tgaccaggat gacctcgagc ggcgtgtcct cgaacacgcg gttgaagcgc
    10921 tccgggtcga tgaagcgcac cgtgagcagc agcgcgtgca gggcgatcga tacgcccagc
    10981 gcgatctgca gcgtggagag gcgcaggagg aaggggggga ctttcatcgc cgggccgccc
    11041 caagatgaaa gagcggcccc ctcggggggc agcgacccgc gaagcggtgg agcgtggggg
    11101 tgaggttcac ggggggcgat tatcccggct ggccggtgcc aggctcgctg cccgccccat
    11161 cggtttcgtt gatgtccacg gcaatggcga tagggccggc cacggtgtct tcctcgtcct
    11221 cgtccacggg gccgtcgtcg ctcgcgtcca gcgggtcgtc caggcgcgcg atcagcgtgc
    11281 cgttcacgtc cagcgtgatc tcgtcgatct cgcccagctt cacgcgcacg cgcgcaccgc
    11341 gcggcaggcc ctgggcaccg agcaccggca gcaccagcgg cagactgtcg gcgcgcacca
    11401 gaaagccgcc gttcatgccc tccttgatga cggtggcgtc cagctcggtg atctggttct
    11461 gctccaggta tttcagcgtc cagaagcgtt ccatgcccgc ctggtagccg ttgtaggcgc
    11521 cgtaggtggc gtcgaagctg ctgatgatgg agaacagctc ggcgtccttc ggcttgaagg
    11581 gcgcggccag cgcggcggtc ttgccgtggc gcgcgcaggc aatgatctgc cactggttca
    11641 ccagatccac atagcggcgc agcggtgatg tggcccaggc gtagctcttg acgccaatgc
    11701 cggcgtgcgg cagcgccttg gtgcccatgc gtaccttgat gccgggggcc aggctggcct
    11761 ggctgcggta gatgccgggc acgccatgct cggccagcat ctggccccaa tggctgttgg
    11821 cgacgatggc ggcctcggcc acgatcaaat cgagcggcgc gccgcgctgg cgcgtgctga
    11881 tgcgcacggt ctcgctgccc tggggttcgg ccttgttatt tccctcaagg cggaagttgt
    11941 agtccgggcg gttgaaagtc tcgggcttgc cgcgcacctg ctcgcgcccg gccttcagtt
    12001 gcttggccag tttatataaa aacgatagct gttcgcgcag ttctagcaag cgctgcggcg
    12061 tgtcttcatg ctgaattgat gcgtcattca gccattgctc ggtgacgata tggtccagct
    12121 ggtcatggcg caggttcacc gtcaccggca cgcgctccag gcgcgtctcg ctggcggtga
    12181 tggccagcgt cgcctcgt
//
LOCUS       DAOMTQ010000092        12087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_958_length_12087_cov_25.410821, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000092 DAOMTQ010000000
VERSION     DAOMTQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_958_length_12087_cov_25.410821"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PK518_09890"
     CDS             complement(<1..810)
                     /locus_tag="PK518_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRM49268.1"
                     /translation="MGLALLPASAALAQDATPGPWPQALQVPGGVARLALGPAPERPV
                     VHAGDIPVLVLGDASAWTAIVGIPLAAPVGQAHVSVQATDGAPARRIAYQVAAKKYRE
                     QRLNVAPRTVDLSPEDQARYERERDHQQRVMATFSTPLPADALRMRQPVPGRRSSSFG
                     LRRVFNGQARNPHSGMDIAAATGTPVVAPLPGRVIDVGDYFFNGGTVWLDHGGGLLSM
                     YCHLSGVDCAMGEQLATGQAFCRVGATGRVTGPHLHWGVMLNRSMVDPALFL"
     gene            1194..2597
                     /locus_tag="PK518_09895"
     CDS             1194..2597
                     /locus_tag="PK518_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRM49269.1"
                     /translation="MRQEHDFIGIKTIPPDAYWGVHTARAVENFPITGQTVAQMPELI
                     RAFAFVKKAAAHANLQLGAINETQANAISKACDDLVAGQLHEQFVVDVIQGGAGTSTN
                     MNANEVIANRALEYLGLHKGRYDVIHPNDHVNASQSTNDTYPTAVKMATYFGIQTLLA
                     ALAELRGAFEAKAREFANILKIGRTQLQDAVPMTLGQEFAAFAAMLADDERRLRESAY
                     LMCEINMGGTAIGTGINAPVGYVDVVTPKLAELSGVPVKAAANLIAATSDTGAFVDIS
                     GVLKRIAAKLSKISNDLRLLSSGPQAGVADIKLPPRQAGSSIMPGKVNPVIPEVMNQV
                     CFEVIGNDVAITMACEAGQLQLNAFEPLVAWALHKSLHHLASACQTLQVNCVEGIVAN
                     QNLLDERIAESVTLVTALNPLIGYEKAAAIAKAAIATGKPIAQVAEDLGIMSQAEMQK
                     LLVAERLTQAGAITSDK"
     gene            complement(2683..3495)
                     /locus_tag="PK518_09900"
     CDS             complement(2683..3495)
                     /locus_tag="PK518_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HRM49270.1"
                     /translation="MNKNQRTTLHTLYVLALAALMGACTSAPSQPAAPMPPTIAQHST
                     PDLASYDWNLTAAFDAQGQPDGRWLLAGREPLQLHFEDQRLSVRKLCNMLGADFGTQG
                     NDIQLTQTMSTKRACADQALMQLEQRVGQLLPSMKRYQLTAGATSRLQLIFADGSRWE
                     LAGQPTPQTQYGGPAERIFLEVAPERVACSHGLMKNAQCLRVREIRYADNGVKQSVGE
                     WQMFYGEIQGWQHEKGMRNVLRINRYAVKTPPADASAYAYVLDMVVETERVK"
     gene            complement(3571..4128)
                     /locus_tag="PK518_09905"
     CDS             complement(3571..4128)
                     /locus_tag="PK518_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRM49271.1"
                     /translation="MNHSHSIAPQTILQFWFDELTPAQHFAKDAQLDAAMGQRFGALL
                     EAGARGELSGWRVNAAGRLAEIVLLDQFSRNIYRDTPQAFAQDGMALVLAQELVTQGL
                     DRQLPSAQRAFAYMPYMHSESRVVQAQSARLFAQPGLEVNLPFALQHQAIIERFGRYP
                     HRNAILGRASSDEELTFLRQPGSAF"
     gene            complement(4125..4982)
                     /locus_tag="PK518_09910"
     CDS             complement(4125..4982)
                     /locus_tag="PK518_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRM49272.1"
                     /translation="MRIAEITTRIVRVVEVPRSQLADFLKTPEAQQVGVYFLLGDMSD
                     AGLPRVYIGQSGTVGTRLAQHNQSKDFWNRALIVISLTNSLTQTHALFLEWFAIAEAT
                     KAARFSLENGNTGTQPYTPAPLQADCHEIHETAALLLATLGQPIFEPLTGGERSENPA
                     LAAEPELFYCKGPDANGIGEYTTEGFVVHKGSTARQAMVASIKDTSNERFRDQLVAEG
                     VLQAQGKSYLFTRDFLFSSPSMAAITVLGRSANGWLEWKTRHGQTLDGLRRQPTDANH
                     LTPLKPLAP"
     gene            complement(5036..6841)
                     /locus_tag="PK518_09915"
     CDS             complement(5036..6841)
                     /locus_tag="PK518_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRM49273.1"
                     /translation="MSTPVHNDTTEPLKPSNFLRQIIDADLAQGTHANNRWAGTPGDA
                     AHQQAGALDPARIRTRFPPEPNGYLHVGHAKSICLNFGLARDYGGVCHLRFDDTNPEK
                     EEQEYVDAIIDAVHWLGFDWKDPDGHENLYFASNYFDFMYRAAEYLIESGNAYVDEQT
                     ADEMRANRGDFGKPGVDSPYRGRTPAENLARFRQMRDGVLADGAAVLRAKIDMASPNI
                     NLRDPAIYRIKRAEHHNTGDKWCIYPMYTFAHPIEDALECITHSICTLEFEDQRPFYD
                     WLLDRLAEGGLIAQPHPRQYEFARLNLTYVVTSKRKLRHLVDNGIVKGWDDPRMPTIA
                     GLRRRGYTPAAIQAFCERIGVTKDYSWIDYATLEGCLREDLEAKAHRAMVVLDPLKLE
                     LTNWAEVFGSGDHLEPCSLPALPHAAEGETVPERRFSLGREVWIEREDFAEVPPKGYK
                     RLFPSNRVRLKGGYVIECTGCEKDAAGQVTKVLATVVPDTKSGTPGADSVKVKAAITW
                     VGVADGVQAEVRLYDRLFTDAQPDAGGKDYLALLNPDSLKVVTAYVEPSLAAAQAEDK
                     FQFERFGYFVADRKDHAPAKPVFNRVTGLKDSWGK"
     gene            7043..7396
                     /locus_tag="PK518_09920"
     CDS             7043..7396
                     /locus_tag="PK518_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49274.1"
                     /translation="MTQRRSWFAAVASAGVALALMAGAGTAQARSDVYWSVGVGAPGV
                     VMGVGNAAPMYYVPPPPVYYAPPPAVYVPPRPVYYAPPPPVYYRGHDHHWRGHHHHDR
                     HRGHDGHRGHRGHRD"
     gene            7523..7969
                     /gene="aroQ"
                     /locus_tag="PK518_09925"
     CDS             7523..7969
                     /gene="aroQ"
                     /locus_tag="PK518_09925"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRM49275.1"
                     /translation="MTKTVYVLNGPNLNLLGTREPQVYGSQTLADVEQLCTEACARHG
                     FTLVFRQSNHEGALVDWIHEAGRLHAAGQLAGLVLNAAAYTHTSVALLDAVKGTGVPL
                     VELHISNVHAREAFRHHSYLSGVARAVMCGFGVQGYALAIDGLAQW"
     gene            7963..8844
                     /locus_tag="PK518_09930"
     CDS             7963..8844
                     /locus_tag="PK518_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM49276.1"
                     /translation="MVSAPALATLPVPAEVLRLQALARRETTPCGDGDMVWHVWGDAR
                     PDRPPLVLLHGGSGSWTHWVRNIDDLLAAGRELWIPDLPGFGASASPTRGGDADALVE
                     PLAEGLRALFGPRPCDLVGFSFGGLTAGLLLAEHPELARQLVLVGAPAMGVVPKRQFE
                     LKAWRHLPADGQLATHRYNLAALMLKDQALIDRLDGLALGLHVANVVRDRMPRRRLAH
                     TDALALALPRVTCPVHAIYGRDDALYKEWIVALEGAYAAAAPDFRGMALIDDTGHWVQ
                     FERPQAFAQTLLAALGA"
     gene            8856..9254
                     /gene="arfB"
                     /locus_tag="PK518_09935"
     CDS             8856..9254
                     /gene="arfB"
                     /locus_tag="PK518_09935"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRM49277.1"
                     /translation="MQVNPAEVELSAMRAQGAGGQNVNKVASAVHLRFDIAASSLPED
                     VKLRLLALRDGRITLQGVLVIKAQQYRTQEANRQDALARLQALVDSVSRPPRPRRATR
                     PTLGSQRRRLESKSRRSGIKALRARPADTQ"
     gene            9303..10118
                     /locus_tag="PK518_09940"
     CDS             9303..10118
                     /locus_tag="PK518_09940"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRM49278.1"
                     /translation="MNQDPQPISPAALPTLYVSHGAPLFALAPGSTGPALTQWGAQLR
                     AQHPDLRGVVIMSPHWMAQGVTVMTGARPATWHDFGGFPPELYQLQYPAPGDPALAGQ
                     VLDLLRQAGMDARGDAARPFDHGAWVPLMHLFPQADLPVVQLALPLGAGPAEVHALGA
                     ALRGLRAQGVLLAGSGSMTHNLAEFFGGVSEPAPYVLEFSRWIEQQLERGDLAALLDY
                     RARAPHARRAHPTEEHFLPLFFALGAAGAGAHAEYLSREVMYGMLAMDAFSLG"
     gene            10250..>12087
                     /gene="aceE"
                     /locus_tag="PK518_09945"
     CDS             10250..>12087
                     /gene="aceE"
                     /locus_tag="PK518_09945"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRM49279.1"
                     /translation="MNAPTPISALNTMPDTDPQETAEWRDAFAALVAAQGPERARFIL
                     DELARAAHARRVGWQPELNTPHVNTIAAQQQPVFPGDLAVEERLASLMRWNALAMVVR
                     ANQAYGELGGHIASYASAADLFEAGFNHFFHAREGLEPGQHRGDLVFFQPHSAPGVYA
                     RAFLEGFLSEQDLLHFRQEITAPTVGVRGLCSYPHPWSMPDFWQFPTGSMGIGPISSI
                     YHARFMHFLTDRGLLDCSGRKVWGVFGDGEMDEPESTSALTLASREGLDNLVWVVNCN
                     LQRLDGPVRGNGSIVAELERLFTGSGWNVIKLMWGSDWDGLFARDLTGALVRTLGETV
                     DGQMQTFAAKDGRYNRDHFFGQSPALQQLAQGMTDEQIDRLKRGGHDLVKIYAAYAQA
                     AAHKGQPTVILAHTKKGYGMGTAGQGKMTTHSQKKLDDQELIEFRNRFNLPLTDAQAV
                     GLEFLKPAPDSAEMQYLQARRAQLGGYLPRRQATCEPLPVPPLASYAQFALQADGKDM
                     STTMAFVRMLGGLLKDASLGPRIVPIVADEARTFGMANLFKQVGIYSNVGQRYAPEDI
                     GSILSYREAKDGQILEEGISEAGAIASWTAAATSYSVHGLAMLPFY"
BASE COUNT         1891 a         3932 c         4160 g         2104 t
ORIGIN      
        1 caggaacagg gccggatcga ccatgctgcg gttcagcatc acgccccagt gcaggtgcgg
       61 gcccgtgacg cggccggtgg cgcccacgcg gcaaaacgcc tggccggtgg ccagctgctc
      121 gcccatggcg cagtccacgc cgctcaggtg gcagtacatg ctgagcaggc cgccgccatg
      181 gtccagccag accgtgccgc cgttgaagaa atagtcgccc acgtcgatca cccggcccgg
      241 cagcggtgcc accacgggcg tgccggtggc ggccgctatg tccatgccgc tgtgcgggtt
      301 gcgcgcctgg ccgttgaaca cgcggcgcag tccaaagctg ctggagcgcc gcccgggcac
      361 gggctggcgc atgcgcaatg cgtcggccgg caacggtgtg gagaaggtgg ccatgacgcg
      421 ctgctggtgg tcgcgctcgc gctcgtagcg ggcctgatcc tcgggcgaaa gatccaccgt
      481 gcgcggcgcc acgttcaggc gctgctcgcg gtacttcttg gccgccacct gataggcgat
      541 gcgccgcgcc ggtgcgccgt ccgtggcctg cacgctgaca tgggcctggc ccacgggcgc
      601 ggccagcggt atgccgacga tggccgtcca ggcgctggca tcgcccagca ccagcaccgg
      661 aatgtcgccg gcatgaacca ccgggcgctc gggcgccggg cccagcgcca gccgggccac
      721 gccgccgggc acctgcagcg cctggggcca tgggcctggg gtggcgtcct gggccagcgc
      781 tgcgctggcg ggcagcagtg ccaggcccat ggcttgcagc atggtgcggc gctggcagcc
      841 ggatctggca gaggggaggt gatggggcag atatggcacg gcgggcagtg tagagcggcg
      901 ccatggcgcc agaggcgggg cgatgcaaaa aggcaaggtt ctcatgccgg ccctgcatat
      961 tgatggataa tttgcgccaa tgccctgggc accaaggtgg tcagggcatt gccacgtctg
     1021 gcgcggcttt catgctgacc cacaaggttc gccgcacggt acatggcacc cgcacagttt
     1081 ttttgcggcc cgctcgcggt ggcttgcatg cgctttggtt gttcagtttt cgcacagccg
     1141 ggcattgttt ttttgtttcc cctccccccc atttgtcaga aagcatatcg accatgcgtc
     1201 aagaacatga cttcattggt atcaagacca tcccccccga cgcctactgg ggcgtgcata
     1261 cggcacgcgc cgtggagaac tttcccatca ccggccagac cgtggcccag atgcccgagt
     1321 tgatccgggc ctttgccttc gtgaagaagg ccgccgccca tgccaacctg cagctgggcg
     1381 cgatcaacga aacccaagcc aatgccattt ccaaggcctg cgacgacctg gtcgccggcc
     1441 agctgcacga gcagttcgtg gtggacgtga tccagggcgg cgccggcacc tcgaccaaca
     1501 tgaacgccaa cgaggtgatc gccaaccgcg cgctggagta cctgggcctg cacaagggcc
     1561 gctatgacgt gatccacccc aacgaccatg tcaacgcctc gcaaagcacc aacgacacct
     1621 atcccacggc cgtgaagatg gccacgtatt tcggcatcca gaccctgctg gcggccctgg
     1681 ccgagctgcg cggcgccttc gaggcgaagg cgcgggagtt cgccaacatc ctcaagattg
     1741 gccgcaccca gctgcaggac gccgtgccca tgacgctggg ccaggagttc gccgccttcg
     1801 ccgccatgct ggccgacgac gagcgccgcc tgcgcgagtc cgcctacctg atgtgcgaga
     1861 tcaacatggg cggcacggcc ataggcaccg gcatcaacgc accggtgggc tatgtggacg
     1921 tggtcacgcc caagctggcc gagctgtcgg gcgtgccggt caaggccgcc gccaacctga
     1981 tcgccgccac ctcggacacg ggcgcctttg tcgacatctc cggcgtgctc aagcgcatcg
     2041 ccgccaagct gtccaagatc agcaacgacc tgcgcctgct gtcctcgggc ccgcaggcgg
     2101 gcgtggccga catcaagttg ccgccgcgcc aggcgggctc gtccatcatg ccgggcaagg
     2161 tgaacccggt gatccccgag gtgatgaacc aggtgtgctt cgaggtgata ggcaacgatg
     2221 tcgccatcac catggcctgc gaggccggcc agctgcagct caacgccttc gagccgctgg
     2281 tggcctgggc cctgcacaag agcctgcacc acctggccag cgcctgccag acgctgcagg
     2341 tcaactgcgt ggaaggcatc gtcgccaacc agaacctgct ggacgagcgc attgccgagt
     2401 ccgtgaccct ggtgacggcg ctgaacccgc tgatcggcta cgaaaaggcc gcagccatcg
     2461 ccaaggccgc catcgccacc ggcaagccaa ttgcccaggt ggccgaagac ctgggcatca
     2521 tgagccaggc cgagatgcag aagctgctgg tggccgagcg gctgacgcag gcgggcgcca
     2581 tcacctcaga caagtaaaac tatcaaaaaa atagctgcta gcgctttttg ataaagcgct
     2641 gcagctgttt ttttatgatg gttttggtgg tcgcttgggg gcttacttca cgcgctccgt
     2701 ctccaccacc atgtccagca cataggcgta ggccgaggca tcggccggcg gggtcttgac
     2761 cgcgtagcgg ttgatgcgca gcacgttgcg catgcccttc tcatgctgcc agccctggat
     2821 ctcgccgtag aacatctgcc attcgcccac cgactgcttc acgccgttgt cggcatagcg
     2881 gatctcgcgc acgcgcaggc attgggcgtt cttcatcaag ccatggctgc aggccacgcg
     2941 ttcgggcgcc acctccagaa agatgcgctc agccggcccg ccgtactgcg tctgcggcgt
     3001 gggctggccg gccagctccc agcggctgcc gtcggcaaag atcagctgca gtcgggacgt
     3061 ggcgccggct gtcagctggt agcgcttcat actgggcagc agctggccca cgcgttgctc
     3121 cagctgcatc agggcctggt cggcgcaggc gcgcttggtg gacatggtct gggtcagctg
     3181 gatgtcgttg ccctgggtgc caaagtcggc gcccagcatg ttgcacagct tgcgcacgga
     3241 caggcgctgg tcttcaaagt gcagctgcag cggctcgcgc ccggccagca gccagcggcc
     3301 gtcgggctgg ccctgggcat cgaaggcggc cgtcaggttc cagtcgtacg aggccaggtc
     3361 gggggtgctg tgctgcgcga tggtcggggg catgggggcc gcgggttgtg atggggcgct
     3421 ggtgcaggcg cccatcaggg cggccagggc caggacgtag agggtgtgaa gggtggtgcg
     3481 ctggttcttg ttcatcggtg ttaggcgcgg ttgcggggca aaggttcggg cgtgaatgtc
     3541 acgcaaacgt caaaccctgc ccggggttgg tcaaaacgcc gaaccgggct ggcgcagaaa
     3601 cgtcagctct tcgtcgctgg atgctcgccc caggatggcg ttgcgatgcg ggtagcggcc
     3661 aaagcgctcg atgatggcct ggtgctgcag cgcaaagggc aggttgacct ccagcccggg
     3721 ctgtgcgaac agccgcgccg actgcgcctg caccacgcgg gattcgctgt gcatgtaggg
     3781 catgtaggca aaggcacgct gggcgctggg caattgcctg tcgagtcctt gcgtcaccag
     3841 ctcctgcgcc agcaccaggg ccatgccgtc ctgggcaaag gcctgcggcg tgtcgcggta
     3901 gatgttgcgt gagaactgat ccagcagcac gatctcggcc agccgcccgg ccgcattcac
     3961 gcgccagcca gacagctcgc cgcgcgcgcc ggcctcgagc agggcgccaa agcgctggcc
     4021 catggcggca tcgagctgcg cgtccttggc gaaatgctgc gccggcgtca gctcgtcgaa
     4081 ccagaactgc aggatggttt gtggagcgat cgagtgcgaa tggttcatgg cgcgagcggc
     4141 ttaagggggg tgagatggtt ggcgtcagtt ggctgtcgcc tcaaaccatc cagtgtctgt
     4201 ccgtgcctgg ttttccattc cagccaaccg ttggctgaac gacccagcac agtgatcgcc
     4261 gccatgcttg gactggagaa gagaaagtcg cgcgtaaaca ggtaactttt accttgtgcc
     4321 tgcaatacgc cctctgcgac caactggtcg cgaaagcgtt cattggacgt gtctttgata
     4381 gaggcgacca ttgcttgcct ggcggtcgag cctttgtgca ccacaaaccc ttcggtcgtg
     4441 tattccccga tgccgttggc atcaggcccc ttgcaataga aaagctcggg ttcagcggcc
     4501 agggccgggt tttctgatcg ctcgccgccg gtcagtggtt caaaaatggg ctggccgagg
     4561 gtggcgagca ggagtgcggc ggtttcgtgt atttcgtggc aatccgcttg caatggcgcc
     4621 ggtgtgtaag gttgggtgcc cgtgttgcca ttctccaggc tgaagcgcgc ggctttggtc
     4681 gcttcagcaa tggcaaacca ttccagaaac aaggcatggg tttgcgtcag gctgttggtc
     4741 agcgaaatca cgatcagagc gcggttccaa aaatccttgc tctggttgtg ctgcgccagc
     4801 cgtgttccta cggttccgga ttggccgata tacacccgtg gcaggccagc atccgacata
     4861 tcgcccagca ggaaatacac gccaacctgc tgcgcttccg gcgtcttgag gaagtctgcc
     4921 aactgactgc gcggcacttc gaccacgcgc acgatgcgtg tggtgatctc ggcgatgcgc
     4981 atgccgcgcg ggttaccggt tgggaggtaa atctggatgg tctgggggcg ggccatcact
     5041 tgccccagga gtccttcagc cccgtcaccc ggttgaacac cggcttggcc ggcgcgtgat
     5101 ccttgcggtc ggcgacgaag tagccgaagc gctcgaactg gaacttgtcc tcggcctggg
     5161 cggcggccag cgagggctcc acataggcgg tgacgacctt caggctgtcc gggttgagca
     5221 gggctaggta gtccttgccg cctgcgtcgg gctgggcgtc ggtaaacagc cggtcgtaca
     5281 ggcgcacctc ggcctgcacg ccgtcggcca cgcccaccca ggtgatggcg gccttgacct
     5341 tgacgctgtc ggcgccgggc gtgccgctct tggtgtccgg caccaccgtg gccagcacct
     5401 tggtgacctg gccggcggcg tctttctcgc agcccgtgca ttcgatgacg tagccgccct
     5461 tgaggcgcac gcggttagac gggaacaggc gcttgtagcc cttgggcggc acctcggcga
     5521 agtcctcgcg ctctatccag acctcgcggc ccaggctgaa gcggcgctca ggcacggttt
     5581 cgccctcggc ggcgtggggc agggcgggca ggctgcaggg ctccaggtgg tcgccgctgc
     5641 cgaacacctc ggcccagttg gtcagttcca gcttgagcgg gtcgagcacc accatggcgc
     5701 ggtgggcctt ggcctccagg tcttcacgca ggcagccttc gagcgtggcg tagtcgatcc
     5761 agctgtagtc cttggtcacg cctatgcgct cgcagaacgc ctgtatggcg gcgggcgtgt
     5821 agccgcgccg gcgcaggccg gcgatggtgg gcatgcgtgg gtcgtcccag cctttgacga
     5881 tgccgttgtc caccaggtgc ctgagcttgc gcttgctggt gaccacgtag gtcaggttca
     5941 ggcgcgcgaa ttcgtattgg cgcggatgcg gctgggcgat caggccgccc tcggccaggc
     6001 gatcgagcag ccagtcgtag aacgggcgct ggtcttcaaa ctccagcgtg cagatgctgt
     6061 gggtgatgca ctccagcgcg tcctcgatcg ggtgcgcgaa ggtatacatc gggtagatac
     6121 accatttgtc gccggtgttg tggtgctcgg cgcgcttgat gcggtagatg gccgggtcgc
     6181 gcaggttgat gttcgggctg gccatgtcga tcttggcgcg cagcacggcc gcgccgtcgg
     6241 ccagcacacc atcgcgcatc tggcgaaagc gtgccaggtt ctccgctggg gtgcggccgc
     6301 ggtaggggct gtccacgccg ggcttgccaa agtcgccgcg attggcgcgc atctcgtcgg
     6361 ccgtctgctc gtccacgtag gcgttgccgg attcgatcag gtactcggcc gcgcggtaca
     6421 tgaagtcgaa gtagttgctg gcgaagtaca ggttctcgtg gccgtccggg tctttccagt
     6481 caaagcccag ccagtgcacg gcatcgatga tggcgtccac gtactcctgc tcttccttct
     6541 cggggttggt gtcgtcaaag cgcaggtggc acacgccgcc atagtcgcgc gccaggccga
     6601 agttcaggca gatgctcttg gcatgaccga catgcaggta gccgttgggc tcgggcggaa
     6661 agcgcgtgcg tatccgcgcc gggtcgagtg cgccggcctg ctggtgggcg gcgtcgccgg
     6721 gggtgccggc ccagcggttg ttggcatgtg tgccctgggc caggtcggcg tcgatgatct
     6781 ggcgcaggaa gttgctgggc ttgaggggtt ctgtggtgtc gttgtggact ggggtgctca
     6841 tgccgcgatt ttaggccgtg tgcttgcagc gcctgtgctg tgcaggggct acgttcggaa
     6901 acaacataaa caggggttca tgacatcctt ttgcccagct ggcgcgttgt ggagtctgac
     6961 aatccttttc atgttcaatg gcgccagtgg ggccgtggcc tgcggagggc aggcaggttt
     7021 caacgtcaaa ggagaatttt tcatgaccca acgtcgctct tggtttgccg ccgtcgcatc
     7081 ggccggtgtg gcattggccc tgatggcggg cgccggcacg gcgcaggcgc gcagtgatgt
     7141 gtattggtcg gtgggcgtgg gtgcgcccgg cgtggtgatg ggtgtcggca atgccgcacc
     7201 catgtattac gtgccgccgc cgcccgtgta ctacgcaccg ccgccggcgg tgtatgtgcc
     7261 gccgcgtccc gtgtactacg cgccgccgcc gccggtgtat taccgcggcc atgaccacca
     7321 ctggcgtgga caccaccacc acgatcgcca tcgtggccat gacggccacc ggggtcaccg
     7381 cgggcatcgc gattgacgct ggcgcagctg gcagacggat gacacaggcg gccctcagtg
     7441 gccgcttttg cttgggcagc ggcccgcata atggacggct tgcgggtccg cgcaggcggg
     7501 gtagaaccgc gtatctcacc gtatgaccaa aaccgtttac gttctcaacg gccccaatct
     7561 caacctgctg ggcacgcgcg agccgcaggt gtatggctcg cagaccctgg ccgatgtcga
     7621 gcagctttgc accgaggcct gcgcacgcca tggtttcacg ctggtgttcc gccaaagcaa
     7681 ccacgagggc gcgctggtgg actggatcca cgaggccggc cgcctgcatg cggcgggcca
     7741 gctggcgggg ctggtgctca acgccgcggc ctatacgcac accagcgtgg cgttgctgga
     7801 cgccgtcaag ggcacgggcg tgccgctggt ggagctgcac atcagcaacg tgcatgcgcg
     7861 cgaggcgttt cgtcaccact cctacctgtc tggcgtggcc cgggccgtga tgtgcggctt
     7921 tggcgtgcag ggctatgccc tggccataga cgggctggcg caatggtgag cgcccccgcg
     7981 ctggccacct tgcccgtgcc cgccgaggtg ctgcgcctgc aggccctggc gcggcgcgag
     8041 accacgccct gcggcgacgg cgacatggtc tggcatgtct ggggcgatgc gcggcccgac
     8101 cggccgccgc tggtgctgct gcatggcggc agcggtagtt ggacgcactg ggtgcgcaac
     8161 atcgacgatc tgctggccgc cgggcgcgag ctgtggattc cggatctgcc cggttttggt
     8221 gcgtcggcgt cgcccacccg gggtggcgat gcagacgccc tggtcgaacc gctggccgag
     8281 ggcttgcgcg cgctgttcgg gccgcgcccg tgcgatctgg tggggttctc gtttggcggc
     8341 ctcacggccg gcctgttgct ggccgagcac ccggagctgg cgcgccagct ggtgctggtg
     8401 ggcgcgccgg cgatgggcgt ggtgcccaag aggcagtttg aactcaaggc ctggcgccac
     8461 ctgccggcag acgggcagct ggccacgcac cgctacaacc tcgcggcgct gatgctcaag
     8521 gaccaggcac tgatcgaccg cctggacggc ctggccctgg gcctgcatgt ggccaacgtg
     8581 gtgcgcgacc gcatgccgcg ccggcgcctg gcgcataccg atgcgctggc gcttgccctg
     8641 ccgcgcgtga cctgtccggt gcatgccatc tacggccgcg acgatgcgtt gtacaaggaa
     8701 tggatagtcg cgctggaggg tgcctatgcc gccgccgcgc cggactttcg cggcatggcg
     8761 ctgatcgacg acaccggcca ctgggtgcag ttcgagcgcc cgcaggcgtt tgcgcagacc
     8821 ctgctggcgg cgctgggggc ttgagtgtgg cggccatgca ggtgaacccg gccgaggtgg
     8881 aattgagcgc catgcgcgcc cagggcgcgg gcgggcagaa cgtgaacaag gtcgcgagcg
     8941 ccgtgcacct gcgttttgac attgccgcgt cctccctgcc cgaggacgtg aagctgcgcc
     9001 tgctggctct gcgcgatggc cgcatcaccc tgcagggcgt gctggtcatc aaggcccagc
     9061 aataccgcac gcaggaggcc aaccgccagg acgcgctggc gcgcctgcag gccctggtgg
     9121 acagcgtgtc gcggccgccg cgcccgcgcc gcgccacgcg gcccacgctg ggctcgcagc
     9181 ggcggcggct ggaatccaag agccggcgtt ccggcatcaa ggcgctgcgt gcccggccgg
     9241 cggatacgca atagggcgcg ggcctcttgg cattgggcgc gccgcgccca catgaggggt
     9301 gtatgaacca agatccgcaa cccatatccc cggcggcgct gccgacgctg tatgtctcgc
     9361 atggtgcgcc gctgtttgct ttggcgccgg gcagcaccgg cccggcgttg acgcagtggg
     9421 gcgcccagct gcgcgcacaa caccccgacc tgcgcggcgt ggtcatcatg tcgccgcact
     9481 ggatggccca aggcgttacc gtcatgaccg gcgcgcggcc cgccacctgg catgactttg
     9541 gtggctttcc gcccgagctc taccagctgc aatacccggc gccgggcgat ccggccctgg
     9601 ctggccaggt gctggatctg ctgcggcagg ccggcatgga tgcccggggc gacgcggcgc
     9661 gccccttcga ccatggcgcc tgggtgccgc tgatgcattt gttcccgcag gccgacctgc
     9721 ccgtggtgca gctggccctg cccctgggcg ccgggccggc cgaggtgcat gccctgggcg
     9781 cggccctgcg cggcctgcgc gcgcagggcg tgctgctcgc gggctcgggc agcatgacgc
     9841 acaacctggc ggaattcttc gggggtgtga gcgagcccgc accctatgtg ctggagttca
     9901 gccgctggat cgagcagcag cttgagcgcg gcgacctggc ggcgctgctg gactaccgcg
     9961 cccgcgcgcc acatgcccgg cgtgcccacc ccaccgagga gcatttcctg ccgctgtttt
    10021 ttgcgctggg ggcggcgggt gcgggcgccc acgccgagta cctgagccgc gaggtgatgt
    10081 acggcatgct cgccatggat gcgttctcct tagggtgaaa ttggcccata gccctttgta
    10141 caaaagcgcg aacagctatc aataaaggag tttcagcact gcgcaatgtc gggcggcagc
    10201 aagcgtctca aatacccttt gtggcgcatt cgggcataca gtggcgtcca tgaatgctcc
    10261 cacgcccatt tccgccctga acaccatgcc ggacaccgat ccgcaagaaa ccgccgaatg
    10321 gcgcgacgcc tttgccgccc tggtggccgc ccaaggcccg gagcgagcgc gcttcatcct
    10381 tgacgagctg gcgcgtgcgg cccatgccag gcgcgtgggt tggcagcccg agctcaatac
    10441 cccccatgtc aacaccatcg cagcgcagca gcagccggtg ttcccgggcg acctggcggt
    10501 ggaggagcgc ctggccagcc tgatgcgctg gaatgccctg gccatggtgg tgcgcgccaa
    10561 ccaggcctat ggcgagctgg gtgggcatat cgccagctac gccagcgcgg ccgatctgtt
    10621 cgaggccggc ttcaaccatt tcttccatgc ccgcgaaggg ctggagccgg gccagcaccg
    10681 cggcgacctg gtgttctttc agccgcacag cgcgcccggc gtgtacgcgc gcgcctttct
    10741 tgagggcttt ttgagcgagc aggatttgct gcatttccgc caggagatca ccgcccccac
    10801 ggtgggcgtg cgcggcctgt gcagctaccc gcacccctgg tccatgccgg atttttggca
    10861 gtttccgact ggctccatgg gcataggccc gatcagcagc atctaccacg cgcgcttcat
    10921 gcacttcttg accgaccgcg gcctgctcga ctgcagcggg cgcaaggtct ggggcgtgtt
    10981 tggcgacggc gagatggacg agcccgagag caccagcgcg ctgaccctgg cctcgcgcga
    11041 gggcctggac aacctggtct gggtggtcaa ctgcaacctg cagcggctcg atggcccggt
    11101 gcgcggcaac ggcagcatcg tcgctgagct cgagcgcctg ttcaccggta gcggctggaa
    11161 cgtcatcaag ctgatgtggg gcagcgactg ggacggcctg ttcgcgcgcg acctgacggg
    11221 cgccctggtg cgcaccctgg gcgagacggt ggacggccag atgcagacct ttgccgccaa
    11281 ggacgggcgc tacaaccgcg accatttctt tggccaaagc cccgcgctgc agcagctggc
    11341 ccagggcatg accgacgagc agatcgatcg cctgaaacgc ggcggccacg acctggtgaa
    11401 gatttacgcc gcctacgcgc aagcggccgc gcacaagggc cagcccacgg tcatcctggc
    11461 gcacaccaag aagggctacg gcatgggcac ggccggccag ggcaagatga ccacgcacag
    11521 ccagaagaag ctcgatgacc aggagctgat cgaatttcgc aaccgcttca acctgccgct
    11581 taccgacgcg caggcggtgg gcctggagtt cttgaagccc gcgcccgaca gcgccgagat
    11641 gcagtacctg caggcgcggc gtgcgcagct gggcggctac ctgccacggc gtcaagccac
    11701 gtgcgagcct ctgccggtgc cgccgctggc cagctacgcc caatttgccc tgcaggccga
    11761 tggcaaggac atgagcacca ccatggcctt tgtgcgcatg ctcggcggcc tgctcaaaga
    11821 cgccagcctg ggcccgcgca tcgtgcccat cgtcgccgac gaggcgcgca ccttcggcat
    11881 ggccaacctg ttcaagcagg tcggcatcta cagcaacgtc ggccagcgct acgcacccga
    11941 ggacataggc tccatcctct cgtaccgcga ggccaaggac gggcagattc tggaagaggg
    12001 catcagcgag gccggcgcca tcgccagctg gacggcggcg gccaccagct acagcgtgca
    12061 cggcctggcc atgctgccgt tctacat
//
LOCUS       DAOMTQ010000093        11987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_975_length_11987_cov_26.526986, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000093 DAOMTQ010000000
VERSION     DAOMTQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_11987_cov_26.526986"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..403)
                     /locus_tag="PK518_09950"
                     /pseudo
     CDS             complement(173..403)
                     /locus_tag="PK518_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044399455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            490..678
                     /locus_tag="PK518_09955"
     CDS             490..678
                     /locus_tag="PK518_09955"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49280.1"
                     /translation="MADYGDGFSVSALKYMRLFYVAYPELLPMQSARTRQTMPGPREA
                     SPGSTCWSPMNRSLQTKG"
     gene            complement(710..2749)
                     /locus_tag="PK518_09960"
     CDS             complement(710..2749)
                     /locus_tag="PK518_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HRM49281.1"
                     /translation="MPTTTIRGACPHDCPDTCALLTTVEDGRAVRVQGNPAHAATGGV
                     LCAKVSRYAERTHHAERILTPLRRSGPKGSGRFTPVSWDEALDDIARRLTEIATRDPQ
                     AILPYSYAGTMGLVQGESMDRRFFHRLGASLLHRSICSTAGGEGLVHTLGGKLGMKVE
                     FFAESKLILIWGSNSIGSNLHFWRIAQQAKRDGARLVCIDPRRTETAEKCHEHLQLRP
                     GTDAALALALMHELIVHDWLDHDYIARHTLGWEALRARALEWPPERAAEVCGLPVEQI
                     RRLARDYGTTRPAAIRLNYGVQRAHGGGNAVRAVACLPALTGAWRERAGGVLLSSSGA
                     FPVQRAALQRPDLLAGRSPRSINMVTIGDDLLRASSPAFGPQIEALVVYNSNPVAVAP
                     DSPKVVQGFAREDLFTVVLEHFQTDTADYADYILPATTQLEHWDVHLAYGHTDVLLNR
                     PAIAPQGQARSNAQIFRDLAARMGFTEPCFADTDEALCRQAYGEKVDFALLLDQGFAP
                     LPVPDAPFADGGFPTPSGKCEFFSQRLADQGLDPLPGHVPNHEAAGASTLYPLAMISP
                     PARNFLNSSFVNLQSLRDIEGRPLLEMHPEDAAARGIADGALVRVFNERGSYECHAAL
                     NGRARPGVVNGLGVWWRKLGHGGTNVNEVTSQALTDMGAGPTFYDCAVQVEAAGG"
     gene            2901..4112
                     /locus_tag="PK518_09965"
     CDS             2901..4112
                     /locus_tag="PK518_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRM49282.1"
                     /translation="MKILDSIAAAASGIAAVRRDIHAHPELCFKEERTADVIAAQLTT
                     WGIPIHRGLGTTGVVGIVHGRDGGACGRAVGLRADIDALPMQEFNTFDHASKYPGKMH
                     ACGHDGHTAMLLAAAQYFSKPEHRDFDGTVYLIFQPAEEGGGGAREMIKEGLFERFPM
                     QAVFGMHNWPGMAAGCFAVSPGPVMASSNEFKITIRGKGSHGAMPHLGIDPVPVACQM
                     VQAFQTIISRNKKPIEAGVISVTMIQAGEATNVVPDFCVLQGTVRTFSVELLDMIERR
                     MRQVAEHTCAAFEARCEFEFVRNYPPTINSVAEAEFARQVMIGIVGEQNVLPQEPTGG
                     AEDFAFMLQAKPGAYVFIANGDGDHRDMGHGGGPCTLHNPSYDFNDALIPLGGTYWVE
                     LARQWLANPTA"
     gene            4234..4491
                     /locus_tag="PK518_09970"
     CDS             4234..4491
                     /locus_tag="PK518_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HRM49283.1"
                     /translation="MSDLNAAFEAAVANSKLLTERPDNPTLLKIYALYKQATAGDNDE
                     KKPSFSDIVGRAKWDAWEKLKGTEGDAARQQYIDLIESLRG"
     gene            complement(4494..5609)
                     /locus_tag="PK518_09975"
     CDS             complement(4494..5609)
                     /locus_tag="PK518_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM49284.1"
                     /translation="MIAAPLRTLPLLATALLLCGCAQSGGSLGYYWQSLRGHLALLQA
                     AEPVQQWLDKPDTPAPLRARLALAQQARAFAVTDLALPDNASYHRFARLERRAAVWNV
                     VAAGPYSLQLHRWCFPVTGCIGYRGYFDEADARTEAAQLAATGLEVNVYGVPAYSTLG
                     YSNWLGGDPLLSTFIAWPEGDFVRLLLHELAHQQVYAAGDTAFNESYATAVERLGVAR
                     WLQQHSTAAARAQFAASEERRAQFRTLTRATRARLAQIYASNNDEAPDYKALSAMKIE
                     AMQQFHADYARLRARWLTAGTAPAQLASLDLWAREANNASFAAQGAYDDLVPTFIALF
                     EREGRDWRRFHAAVRLLAEQPQDARDAALKALAPAGD"
     gene            5650..6300
                     /locus_tag="PK518_09980"
     CDS             5650..6300
                     /locus_tag="PK518_09980"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRM49285.1"
                     /translation="MPEDVLDSSGLFTDEAGHARCFWCRASPLYRHYHDHEWGFPVVD
                     ERRLFEKLCLEGFQAGLSWLTILNKREAFRAAMANFEAEELARFGATEVAQLLGNAAI
                     VRHRGKIASAIQNARRVLELKREFGSLAAYVWRYAPQAGEGRPAHMTLAAVRALTQSA
                     ASVALSRDLKRRGFSFVGPTTMYAFMQATGLVNDHIEGCGARAAAQAARAAFVPPQ"
     gene            complement(6344..6505)
                     /locus_tag="PK518_09985"
     CDS             complement(6344..6505)
                     /locus_tag="PK518_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49286.1"
                     /translation="MGNKIVTEADRKRTPRPTPMPGMPVATQGRGQRVSLERGVATRS
                     KKNPGKRHG"
     gene            6765..8075
                     /locus_tag="PK518_09990"
     CDS             6765..8075
                     /locus_tag="PK518_09990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM49287.1"
                     /translation="MRHLLDSLQASLALSPVGIAVQLPGGLRLGDANAELRLQFRDRM
                     GVVALAMGEIGHVGTAIVEGRVLLQGSMRQLMAAAAALLHSDPARDEQTRWWRRVLLR
                     ARSMAAHTRSRDARHIARHYDLSDDFYALWLDPQRVYSCAYFSVDGMTLAQAQQAKLD
                     HICRKLMLRPGERFLDMGAGWGGLMLWAAEHYGVDATGITLSRNQHAHVQRLIAARGL
                     QGRVRMQLLDYRALQVDAPFDKIASVGMFEHVGHAQMGQYFATLQRLLRPGGLVMNHG
                     ITAGGLDNAAGLGAGLGDFIERYIFPGGELLHVSQALQELARAGLEMLDVENLRPHYA
                     RTLWAWSDALEARLDQARRILHAQQGPEQGEKTLRAYRLYLAGSALAFEHGWIALHQI
                     LAARPTGVVEGAVLKGAQSDYPFNRAYMYEDRAAARAAPPDMLP"
     gene            8089..8415
                     /locus_tag="PK518_09995"
     CDS             8089..8415
                     /locus_tag="PK518_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HRM49288.1"
                     /translation="MLFKFKSRATADLIMLEPNGRRVLEIIGKNPDDAHGIVTAEQIP
                     AAIAALEAAVRADDAARTAPADDEEGADDEGQGTQRDPVSLRQRAAPFIDMLRRSAAE
                     AHDVVW"
     gene            complement(8437..9453)
                     /locus_tag="PK518_10000"
     CDS             complement(8437..9453)
                     /locus_tag="PK518_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49289.1"
                     /translation="MPINTPRRRALNTLAAALALTLGAAGMAQAQSHWPAKPVRIVVP
                     FAAGGTTDILARAIAPELGKALGQQFIVDNRGGAGGNIGADLVAKSAGDGYTLLMGTV
                     GTHGINRALYQQLPYDPIKDFAPITLVASVPNVMVMNADKARQLGIANVQDFIRYARA
                     NPGRLNMASSGNGTSIHLAGELFKSMTGTYMLHMPYRGSAPALLDMVGGNMDVMFDNL
                     PSSMVHIKAGKLKALAVTSASRSAALPDVPTVEEAGGAALKDYEASSWFGLLAPAATP
                     PEVVQRLQQEVAKALASPAVKERLLAQGAIPGGNSSADFVRMIDSEHRKWAQVVKAAG
                     AKVD"
     gene            complement(9596..10586)
                     /locus_tag="PK518_10005"
                     /pseudo
     CDS             complement(9596..10586)
                     /locus_tag="PK518_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     assembly_gap    10697..10796
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10797..11984)
                     /locus_tag="PK518_10010"
     CDS             complement(10797..11984)
                     /locus_tag="PK518_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM49290.1"
                     /translation="MAALPRQIAHAQQSSPAFASILAGVDAASITSRAALAQLPVTRK
                     YQLHERQQAERAHNAFGGFASIGFGAAMPRVFASPGPIYEPEGARADYWRMARALYAA
                     GFRAGELAHNCFSYHFVPAGSMMESGAHALGCTVFPGGTGQTEQQVQAMAELRPAGYI
                     GTPSFLKIILEKATEMGVQLPSVTKALLSAEAFPPSLRDWLAQQGVQGYQCYGTADLG
                     LIAYETSAREGLVLDEGVIVEIVRPGTGDPVPEGEVGELVITTLNPDYPLIRFGTGDL
                     SAVLPGSCPTGRTNTRIKGWLGRADQTTKVRGMFVHPGQIDQVVKRFPQVTKARLVVS
                     GEMANDQMTLQVETTETAPGLAQLLAEALREVTKLRGEVEMLAPGGLPNDGKVIEDAR
                     SYR"
BASE COUNT         1841 a         3888 c         4199 g         1959 t
ORIGIN      
        1 gggaattttt cgccagtttg tggccgaaca tctgcatgac tgagagtctc ttggggacta
       61 cgctgagttt ccaaagcttg gacttgaagt tgcccctttt tcacggacac cttaccgttt
      121 ggacaaaagg agcagaaaag ttaggctctt ttcgcgaaac gtacggatca gctcaagccc
      181 tgatagccaa aaccagcact gacgcgggct ccggggcaga tccgggcgcg cgatccagca
      241 tgcgaaacca gcccaggtaa gacgccaggt acttggtggc cacgcccttg aagcgccgca
      301 tccagctgcg caggcggctg acgtaggcgt tgacgttctg cacatgccaa ggcccgaccc
      361 gaacgccagc gttgacgttg acgctgtggt attgcacgcc cagcaggtgg gccgcagccg
      421 ccagcgctgc gctgagctcg gctgcagtgg tatggcaagg cgctgctggc gtctttgtct
      481 gcccggctga tggcggacta cggggacggg ttttctgtgt ccgcgctcaa gtacatgcgg
      541 cttttttatg tggcgtaccc agagcttttg ccgatgcaga gtgcaagaac gcgacagacc
      601 atgcccggtc cgcgggaggc aagccctgga agtacctgct ggtctcccat gaacaggtca
      661 ctgcagacaa aaggctgagc gaccttttga ggtttgaagt cgagagccgt caaccgcccg
      721 ccgcctcgac ctgcacggcg cagtcgtaga acgtcggccc cgcgcccatg tccgtcagcg
      781 cctggctggt gacctcgttg acattcgtgc cgccatggcc cagcttgcgc caccacacgc
      841 ccaggccgtt gaccacgccg gggcgggcgc ggccgttgag cgcggcgtgg cattcgtagc
      901 tgccgcgctc gttgaacacg cgcaccaggg cgccgtcggc aatgccgcgc gcggcggcgt
      961 cctcggggtg catctccagc agcgggcggc cctcgatgtc gcgcaggctt tgcaggttca
     1021 cgaagctgga gttcaaaaag ttgcgcgccg gcggcgagat catggccagc gggtacagcg
     1081 tcgatgctcc cgccgcctcg tgattcggca catggccggg cagcggatcg agcccctggt
     1141 cggccaggcg ctggctgaaa aattcgcact tgcccgaggg cgtgggaaag ccgccatcgg
     1201 caaacggcgc gtcggggacg ggaagcgggg caaagccttg gtcgagcagc agcgcgaagt
     1261 ccaccttctc gccataggcc tggcggcaca gcgcttcgtc ggtgtcggca aagcagggct
     1321 cggtgaaccc catgcgcgcc gccaggtcgc ggaagatctg cgcgttgctg cgcgcctggc
     1381 cctgcggcgc aatcgccggg cggttgagca gcacgtcggt gtggccgtag gccaggtgca
     1441 cgtcccagtg ctcgagctgc gtggtggcgg gcagtatgta gtcggcgtaa tcggcggtgt
     1501 cggtttggaa atgctccagc accacggtga acaaatcctc gcgcgcaaag ccctgcacca
     1561 ccttgggcga atccggcgcc acggccacgg ggttgctgtt gtagaccacc agggcttcga
     1621 tctgcgggcc gaaggccggg gatgaggcgc gcagcaggtc gtcgccaatg gtcaccatgt
     1681 tgatgctgcg cgggctgcgg ccggccagca aatctgggcg ctgcagtgcc gcgcgctgca
     1741 ccggaaaggc gcccgagctc gacagcagta cgccgccggc gcgctcgcgc caggcgccgg
     1801 tcagcgccgg caggcaggcc acggcgcgca cggcattgcc gccgccatgg gcgcgctgca
     1861 cgccgtagtt caggcggatg gcggcgggcc gggtggtgcc gtagtcgcgc gccaggcgcc
     1921 ggatctgctc cacgggcagg ccgcagacct cggcggcgcg ctcgggcggc cattcgaggg
     1981 cgcgtgcgcg caaggcctcc cagcccagcg tgtggcgcgc gatgtagtcg tggtccagcc
     2041 agtcgtgcac tatgagttcg tgcatcagcg ccagggccag cgcggcgtcg gtgccggggc
     2101 gcagctgcag gtgttcgtgg catttctcgg cggtctcggt gcggcgcggg tctatgcaca
     2161 ccaggcgcgc gccgtcgcgc ttggcctgct gggcgatgcg ccagaaatgc aggttgctgc
     2221 caatcgaatt gctgccccag atgaggatga gtttggactc ggcaaagaac tcgaccttca
     2281 tgcccagctt gccgcccagc gtgtgcacca ggccctcgcc accggcggtg gagcagatgc
     2341 tgcggtgcag cagggatgcg cccaggcggt ggaagaagcg ccggtccatg ctctcgccct
     2401 gcaccaggcc catggtgccg gcgtagctgt agggcaggat ggcctgcggg tcgcgtgtgg
     2461 cgatctccgt caggcgccgg gcgatgtcgt ccagggcctc gtcccaggac acgggcgtga
     2521 accggccgct gcccttgggg ccgctgcgcc tgagcggggt gaggatgcgc tcggcatggt
     2581 gcgtgcgctc ggcatagcgc gacaccttgg cgcagagcac gccgccggtg gccgcatgcg
     2641 ccgggttgcc ctgcacgcgg acggcgcgcc cgtcctccac ggtggtcagc agggcgcagg
     2701 tgtcggggca gtcgtgcggg caggcgccgc gtatggtggt cgtgggcatg gctgagggag
     2761 tcgctggggc aactgcgcat ttcaccatga agcagcgccg cggacgagac catggtttct
     2821 ggcatacccc gtggtttggc aggcttggct ggcagggcta gactgtgacg cttcccaagg
     2881 aaacaagcgc cgagcacatc atgaaaatcc tagactccat cgccgcagcg gcctccggca
     2941 tcgccgccgt gcgccgagac atccatgccc accccgagct gtgcttcaag gaagagcgca
     3001 ccgccgacgt gatcgccgcc cagctcacca catggggcat tcccatccac cgcggcctgg
     3061 gcaccaccgg cgtggtgggc atagtccatg gccgcgacgg cggcgcctgc gggcgcgccg
     3121 tgggcctgcg cgccgacatc gacgccctgc cgatgcagga gttcaacacc ttcgaccatg
     3181 ccagcaagta cccgggcaag atgcacgcct gcggccacga cggccacacg gccatgctgc
     3241 tggcggcggc gcagtatttc tccaagcccg agcaccgcga cttcgacggc accgtctatc
     3301 tgatcttcca gccggccgag gagggcggcg gcggcgcgcg cgagatgatc aaggagggcc
     3361 tgttcgaacg cttccccatg caggccgtgt ttggcatgca caactggccc ggcatggccg
     3421 cgggctgctt tgccgtcagc cccgggccgg tgatggcgtc ttccaacgag ttcaagatca
     3481 ccatccgcgg caagggcagc catggcgcca tgccgcatct gggcatagac ccggtgccgg
     3541 tggcctgcca gatggtgcag gccttccaga ccatcatcag tcgcaacaag aagcccatcg
     3601 aggccggcgt gatctcggtc accatgatcc aggccggcga ggccaccaac gtggtgcctg
     3661 atttttgcgt gctgcagggc acggtgcgca ccttctcggt cgagctgctg gacatgatcg
     3721 agcggcgcat gcgccaggtg gccgagcaca cctgcgcggc cttcgaggcg cgctgcgagt
     3781 tcgagttcgt gcgcaactac ccgccgacca tcaactccgt ggccgaggcc gagttcgcgc
     3841 gccaggtgat gatcggcatc gtcggcgagc agaacgtgct gccccaggag cccaccggcg
     3901 gcgccgagga cttcgccttc atgctgcagg ccaaacccgg cgcgtacgta ttcatcgcca
     3961 atggcgacgg cgaccaccgc gacatgggcc atggcggcgg cccctgcacg ctgcacaacc
     4021 cgagctacga cttcaacgac gcgctgatcc cgctgggcgg cacctactgg gtggagctgg
     4081 cgcgccagtg gttggccaac ccgaccgcct gacgcctgcc gactggacaa aaaactccca
     4141 ccctgtgcgg tgggtgtttt ttgcctataa aaaagaacga tcgttcgtat ttgccgctac
     4201 actggctcaa tcaccactac atcggagacc gccatgtccg acctgaatgc cgcttttgaa
     4261 gccgccgtcg ccaactccaa gctgctcacc gagcgcccgg acaaccccac gctgctgaag
     4321 atctacgccc tgtacaagca ggccacggcc ggtgacaacg atgagaagaa gcccagcttc
     4381 tccgacatcg tcggccgcgc caaatgggac gcctgggaaa agctcaaggg caccgagggc
     4441 gacgccgcca ggcagcagta catcgacctg atcgagtcgc tgcgcggttg aggtcaatcg
     4501 cccgcgggcg ccagggcctt gagcgcggca tcgcgcgcat cctgcggttg ctctgccagt
     4561 agccgcacgg cggcatgaaa gcgccgccag tcgcggccct cgcgctcgaa cagggcgatg
     4621 aaggttggca ccagatcgtc ataggcaccc tgggcggcaa aactggcgtt gttcgcttcg
     4681 cgcgcccaca gatccaggct cgcgagctgg gccggcgcgg tgccggcggt cagccagcgg
     4741 gcgcgcagcc gcgcatagtc cgcgtgaaac tgctgcattg cttctatttt catagctgac
     4801 agcgctttgt agtcgggcgc ttcatcgttg tttgatgcat aaatctgggc cagccgtgcg
     4861 cgcgtggccc gggtcagggt gcggaactgc gcgcgccgct cctcgctggc ggcaaattgg
     4921 gcgcgcgccg cggccgtgga atgctgctgc agccagcggg ccacgcccag gcgctccacg
     4981 gccgtggcgt aggactcatt gaacgccgtg tcacccgcgg catagacctg ctggtgcgcc
     5041 agctcatgga gcagcaggcg tacaaagtcg ccctcgggcc aggcaatgaa ggtagacaag
     5101 agcgggtcgc cgcccagcca gttgctgtag cccagcgtgg aataggccgg cacgccgtag
     5161 acgttcacct ccaggccggt ggcggccagc tgggcggcct cggtgcgtgc gtcggcctcg
     5221 tcgaagtagc cgcggtagcc gatgcagcca gtgacgggga aacaccagcg gtgcagctgc
     5281 agcgagtacg gcccggctgc caccacgttc cacacggcgg cgcggcgctc caagcgggca
     5341 aagcggtggt agctggcgtt gtcgggcagg gccaggtcgg tgacggcaaa ggcgcgcgcc
     5401 tgctgcgcca gtgccagccg cgcgcgcagc ggcgccgggg tgtcgggctt gtccagccat
     5461 tgctgcaccg gctccgcggc ctgcagcagc gccaggtggc cgcgcagcga ctgccagtag
     5521 tagcccagac tgccgcccga ctgcgcgcag ccacacagca gcagggcggt ggccagcaag
     5581 ggcagggtgc gaagcggcgc ggcgatcatg gccggccagc atagcaagcg cgccggcgca
     5641 caatgcggca tgcctgaaga tgttttggat agcagcggcc tgttcaccga cgaggccggc
     5701 catgcgcgct gcttctggtg ccgcgcctcg ccgctgtacc gccactacca cgaccatgag
     5761 tggggctttc ctgtcgtgga cgagcgccgc ctgttcgaga agctgtgcct ggagggcttc
     5821 caggcaggcc tgtcctggct caccatcctg aacaagcgcg aggcctttcg cgccgccatg
     5881 gcgaatttcg aggccgagga gctggcgcgc ttcggcgcga ccgaggtggc tcagctgctg
     5941 ggcaatgcgg ccatcgtgcg ccaccgcggc aagattgcct cggccatcca gaacgcgcgc
     6001 cgcgtgctgg agctcaagcg tgaattcggc tcgctggccg cctatgtctg gcgctatgcg
     6061 ccgcaggccg gcgaaggccg gcccgcgcac atgacgctgg cggccgtgcg cgcgctcacc
     6121 cagtcggcgg cgtcggtggc gctgtcccgg gatttgaagc ggcgcggctt cagctttgtc
     6181 ggcccgacca ccatgtacgc cttcatgcag gccacgggcc tggtcaacga ccatatcgaa
     6241 ggctgcggcg cgcgcgccgc ggcccaggcg gcgcgcgccg cctttgtgcc accccaatga
     6301 aaacagccct ccgaggaggg ctgtttcacg gcgagggaac ggctcaaccg tggcgcttgc
     6361 cggggttctt cttgctgcgc gtggccacgc cgcgctccag gctcacgcgc tggccgcggc
     6421 cttgcgtggc cacgggcatg ccgggcatgg gggtgggacg gggagtgcgc ttgcggtcgg
     6481 cttcggtgac gattttgttg cccatgatgg ctcctgcggt gaattggcga tgaaaacccg
     6541 ctattaggcc atatttgcaa acatccctgg ccaaatgggg ggcgaagcac ctggctgggt
     6601 gcagccaagc caggcattga tactattaaa taaagagctg tttgcgcttt tcaggtaagg
     6661 gctgaggccg attttttact ttaatcgccc catggcttgc gccgattgac gcgtcggggc
     6721 cgcggcgaga agaatcgcgc ctgtcgttca ggaaggggac atgcgtgagg catttgctcg
     6781 attccctgca agccagtctg gcgctgtcgc cggtgggcat tgccgtgcaa ttgccgggtg
     6841 gcctgcgcct gggggacgcg aacgccgagc tgcgcctgca gtttcgcgac cgcatgggcg
     6901 tggtggcgct ggccatgggc gagatcggcc atgtgggcac ggccatcgtc gagggccgcg
     6961 tgctgctgca gggcagcatg cgccagctca tggccgcggc cgcggccctg ttgcactccg
     7021 acccggcgcg cgacgagcag acccgctggt ggcgccgcgt gttgctgcgc gcgcgctcca
     7081 tggcggcgca cacgcgatcg cgcgatgcgc ggcatatcgc gcggcattac gacctgtccg
     7141 acgacttcta cgcgctgtgg ctggatccgc agcgcgtcta ttcctgcgcc tacttttccg
     7201 tcgatggcat gacgctggcc caggcgcagc aggccaagct tgaccatatc tgcaggaagc
     7261 tgatgctgcg ccccggcgag cgttttctgg acatgggcgc gggctggggt ggtctgatgc
     7321 tgtgggcggc cgagcattac ggcgtggacg ccaccggcat caccctgtcg cgcaaccagc
     7381 atgcccatgt gcagcgcctg atagcggcgc gcggcctgca gggcagggtg cgcatgcagc
     7441 tgctggacta ccgcgcgctg caggtcgatg cgccgttcga caagattgcc tcggtcggca
     7501 tgttcgagca tgtgggccat gcgcaaatgg ggcagtactt tgccactctg cagcgcctgc
     7561 tgcgccccgg cggcctggtg atgaaccacg gcatcacggc cggcgggctg gacaacgccg
     7621 ccggcctggg cgctggcctg ggcgacttca tagagcgcta catcttcccc ggtggcgagc
     7681 tgctgcatgt gagccaggcg ctgcaggagc tggcgcgcgc cggcctggaa atgctggatg
     7741 tggagaacct gcgcccgcac tacgcgcgca cgctgtgggc ctggtcggac gcgctggagg
     7801 ccaggctgga tcaggcgcgg cgcattctgc acgcccagca ggggccggag cagggcgaaa
     7861 agacgctgcg tgcctaccgc ctgtacctgg cgggcagtgc cctggccttc gagcatggct
     7921 ggattgccct gcaccagata ctggcggcgc ggcccacggg ggtggtcgag ggggcggtgc
     7981 tcaagggcgc gcagtcagac tatcccttca accgcgccta catgtacgaa gatagggctg
     8041 cggcgcgcgc cgcacctcct gacatgctgc cttgaactgg actttcacat gctcttcaaa
     8101 ttcaaatccc gcgccaccgc cgacctcatc atgctggagc ccaatggccg gcgggtgctg
     8161 gaaatcattg gcaagaaccc cgacgacgcc catggcatcg tcaccgcaga gcagatcccg
     8221 gccgccatcg ccgcgctgga ggcggcggtg cgtgccgacg atgcggcgcg caccgccccg
     8281 gccgatgacg aggagggcgc ggacgacgag ggccagggca cgcagcgcga cccggtgagc
     8341 ctgcgccagc gtgccgcgcc cttcatcgac atgctgcggc gcagcgccgc cgaagcgcac
     8401 gacgtggtct ggtgagccgc gccgctggcg cgcgggtcaa tcgaccttgg cgcccgcggc
     8461 cttgaccacc tgggcccact tgcggtgctc gctgtcgatc atccgcacga aatcggcgct
     8521 gctgttgccg ccggggatgg cgccctgggc cagcagccgc tccttgacgg ctggcgaggc
     8581 cagggccttg gccacctcct gctgcaggcg ctgcaccacc tcgggcggcg tggccgccgg
     8641 cgccagcagg ccgaaccagg agctggcctc atagtccttg agtgcggcgc cgccggcctc
     8701 ctccaccgtg ggcacgtcgg gcagggcggc cgagcggctg gcgctggtca cggccagcgc
     8761 cttgagcttg ccggccttga tgtgcaccat ggaggagggc aggttgtcga acatcacgtc
     8821 catgttgccg cccaccatgt ccagcagcgc cggggccgag ccgcggtagg gcatgtgcag
     8881 catgtaggtg ccggtcatgc tcttgaacag ctcgcccgcc aggtggatgg aggtgccgtt
     8941 gccgctggag gccatgttca gcctgcccgg gttggcgcgg gcgtagcgaa tgaagtcctg
     9001 cacgttggca atgcccagct ggcgcgcctt gtcggcgttc atcaccatga cgttgggcac
     9061 cgaggccacc agcgtgatgg gcgcgaagtc cttgatcggg tcatagggca gctgctggta
     9121 cagcgcgcgg ttgatgccat gcgtgcccac cgtgcccatg agcagggtgt agccgtcgcc
     9181 ggccgacttg gccaccagat ccgcgccgat attgccgccg gcgccgccgc ggttgtccac
     9241 gatgaactgc tggcccaggg ccttgcccag ttcgggcgcg atggcgcgcg ccaggatgtc
     9301 ggtggtgccg ccggcggcaa agggcacgac gatgcgcacc ggcttggccg gccaatggct
     9361 ttgcgcctgc gccatgccgg ccgcgcccag ggtcagggcg agggcggcgg ccagtgtgtt
     9421 cagggcccgg cggcgtgggg tgttgatggg catgagtcct cccttgtatg gacgaagaaa
     9481 cgccgtttgt atgtccgcag gggcgggcca tgccagcagg gtaacccgca ggcggcaaga
     9541 aaaaacgccc ggtgcgggca caccgggcgt tccttgtgtt ggcgctggct gcgggtcagt
     9601 cggcgtaggc gccggcggcc ttgatgacca cgccccattt ctcgatctcg gcctgcacga
     9661 acttcttgtg ctcggccggc tcgacgacgc ttgtcggcca ccaccacggc gcccaggcct
     9721 tcctgcttct tgatgaattc aggatctttc acggccacct gcagggcgtc gttgatggcc
     9781 ttgaccacgg cggcgggcgt gcccttgggg gcgtacaggc catgccagac ggagacgttg
     9841 aagtccttca ggccgccctc ctgcagcgtc ggcaggtctt tcagtgccgg catcgtcagg
     9901 cgcttgctgg tggtcacggc aaaggccttg accttcttgc cctcgatctg cgacgtggtg
     9961 ttggtggtct ggtcgcacat caggtcgatc tggccgccga tcaggtcggt gatggccggc
    10021 gccgcgccct tgtaggccac gggcgtcatg tgcgactgca gcgcgtgctg gaacatcagc
    10081 ccgcacaggt ggccggccga gcccaggccg gaatggccca ggttgacctt ttccttgttc
    10141 tgcgcgatcc aggaggccag ctccttgtag ttcttcgcct ccagcgtggg gcgcgagatg
    10201 atggtcatgg gcacgtcgtt gacgatgccc aggaactcga agtcgttgag cacgttgaac
    10261 ggcagcttgc ggtacagcgt ggggatggtg gccatgccga tgtggttcag cagcagggtg
    10321 tagccgtcgg gctgggcacg ggccaccttg gccgcgccaa tggtgctgcc ggcgccggcc
    10381 gagttgtcga tgaccacggt ggcgccgccc aggggcttgc gcagggcctc ggccaggtcg
    10441 cgcgccacgc ggtcggtggg cccgccggcc gagaagggca cgacgatggt gatggacttg
    10501 cccgcggccg ggaaggtttg ggccgacgcg ccgaaggcgg ccaggatggc ggccgtgccc
    10561 agggcgagct tgaaggtggt gtgcatggag cttccttgta aaagacgcgc aatgataggc
    10621 aggatggggt gggcggacga tgcagaaaaa caccaagtcc accaggccgc tgccctcgcc
    10681 ggcgcgcgca gtctatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcag
    10801 cggtagctgc gggcgtcctc gatgaccttg ccgtcgttgg gcaggccgcc cggggcaagc
    10861 atctcgacct cgccgcgcag cttggtgacc tcgcgcagcg cctctgccag cagctgggcc
    10921 aggccgggtg cggtctcggt ggtttccacc tgcagcgtca tctggtcgtt ggccatttcg
    10981 ccgctgacca ccaggcgcgc cttggtgacc tgcgggaagc gcttgaccac ctggtcgatc
    11041 tggccagggt gcacgaacat gccgcgcacc ttggtggtct ggtcggcgcg gccgagccag
    11101 cccttgatgc gggtgttggt gcggcccgtg gggcagctgc cgggcagcac ggccgacagg
    11161 tcgccggtgc caaagcggat cagcgggtag tcggggttca gcgtggtgat caccagctcg
    11221 ccgacctcgc cctcgggcac cgggtcgccg gtgccggggc gcacgatctc cacgatcacg
    11281 ccctcgtcca gcaccaggcc ttcgcgcgcg ctggtctcgt aggcgatcag gcccaggtcg
    11341 gccgtgccgt agcactggta gccctgcacg ccctgctgcg ccagccagtc gcgcagcgag
    11401 ggcgggaagg cctcggccga cagcagcgcc ttggtcacgc tgggcagctg cacgcccatc
    11461 tcggtcgcct tctccaggat gattttcaga aagctgggcg tgccgatgta gccggcgggc
    11521 cgcagctcgg ccatggcctg cacctgctgc tcggtctggc ccgtgccgcc ggggaacacc
    11581 gtgcagccca gggcgtgcgc gcccgattcc atcatcgagc ccgcgggcac gaagtggtag
    11641 gagaagcagt tgtgcgccag ctcgccggcg cgaaaaccgg ccgcgtacag cgcgcgcgcc
    11701 atgcgccagt agtcggcgcg tgcgccttcg ggctcataga tggggccggg gctggcgaac
    11761 acgcgcggca tggcggcgcc aaagcctatg ctggcaaagc cgccaaaggc gttgtgcgcg
    11821 cgctcggcct gctggcgctc gtgcagctgg tacttgcgtg tcacgggcag ttgcgccagt
    11881 gcggcgcggc tggtgatgct ggcggcatcc acgccggcca ggatgctggc aaacgccggg
    11941 ctgctctgct gggcgtgggc aatctggcgc ggcagcgcgg ccatgtg
//
LOCUS       DAOMTQ010000094        11966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_978_length_11966_cov_25.908488, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000094 DAOMTQ010000000
VERSION     DAOMTQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_978_length_11966_cov_25.908488"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="PK518_10015"
     CDS             <1..153
                     /locus_tag="PK518_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRM49291.1"
                     /translation="LPSVDHPVHGRHVELGVKGAAALVPAAWQEMHQALHQFGAVMGP
                     ETVHHL"
     gene            260..1675
                     /gene="glnA"
                     /locus_tag="PK518_10020"
     CDS             260..1675
                     /gene="glnA"
                     /locus_tag="PK518_10020"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRM49292.1"
                     /translation="MAKTVADVMKMVEENEVKFVDLRFTDTRGKEQHVTVPVSHFDED
                     KFSSGHAFDGSSVAGWKGIEASDMQLMPDPSTANIDPFFEETTLFLQCDVVEPGDGKA
                     YDRDPRSIAKRAEAYLKASGLGDTAYFGPEPEFFIFDGVRWSNEPGKVFFEIEEYEAP
                     WNSGTKFESGNRGHRPTTKGGYFPVPPVDSTQDMRAEMVLILEQLGIPVEVFHHEVAG
                     AGQNEIGTRFSTLVERADWTQVLKYVVWNVANAYGKTATFMPKPYAGDNGSGMHVHQS
                     IWKDGKNLFAGDGYAGLSDFALYYIGGIIHHARALNAITNPGTNSYKRLVPGYEAPVK
                     LAYSSRNRSASIRIPYVANPKGRRIEARFPDPLANPYLCFSALMMAGLDGVENKIHPG
                     EAATKDLYHLPPEEDKLVPTVCHSLDQALEALDKDRGFLTKGGVFSDNMLDAYIELKM
                     GEVQRFRMAPHPVEYDMYYSL"
     gene            1882..2385
                     /locus_tag="PK518_10025"
     CDS             1882..2385
                     /locus_tag="PK518_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49293.1"
                     /translation="MKHCLFALTVIAAMLQPAWAQDRIYRCGNEYTNNAAQAKERGCK
                     LVEGGNVTVVEGTRPAAAPSGGAAASSASKAAASPPNAPRVAATEQRARDADARAILQ
                     DELRKAESRLADLRKEYNDGAPQRSALELRNPQGYIERTAELKAAVTRAEADVAGIKR
                     ELGRLGN"
     gene            2404..3471
                     /gene="glnL"
                     /locus_tag="PK518_10030"
     CDS             2404..3471
                     /gene="glnL"
                     /locus_tag="PK518_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HRM49294.1"
                     /translation="MPAPPAPPRYQALDLLSTLVAVLDAQGRVLYVNAALENALGLSR
                     RMLLGADFAALFGDPATVQTALAAVRAQDYAQMRCEAALRRPQQEPLPVHAHIAPAEH
                     AGEQVVELWPLLAQAHQDREERLLEQAQANKELIRNLAHEIKNPLGGIRGAAQLLQLE
                     LASPELAEYTQVIIHEADRLQHLVDRLLAPHRQPRLVGDVNIHEVCEHVCRLVLAEHP
                     RGLVIQRDYDTSIPEFRGDRAQLVQALLNIVQNAAQALGERIAAGDAQITLRSRVARQ
                     VTLGRQRHRLALELHVIDNGPGVPEAIKERIFYPLVTGRGGGSGLGLPLAQAFVQHHQ
                     GMIDCESRPGCTDFRILIPLP"
     gene            3494..4936
                     /gene="ntrC"
                     /locus_tag="PK518_10035"
     CDS             3494..4936
                     /gene="ntrC"
                     /locus_tag="PK518_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HRM49295.1"
                     /translation="MKPIWIVDDDASIRFVLEKALAREHLPTRSFTQPREVLDALAEA
                     GEPGPAALVSDIRMPGGSGLQLLEQLRARLPGLPVIIITAYSDLDSAVSAYQGGAFEY
                     LPKPFDVPKAVELIRRAVQESQREEVAEQQLAATPEMLGQAGAMQDVFRAIGRLSQSA
                     VTVLITGESGTGKELVARALHKHSPVAGGPFVAINTAAIPKDLLESELFGHERGAFTG
                     AQAQRRGRFEQAEGGTLFLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAPVKAHVRVI
                     AATHQNLEQRVKDGSFREDLLHRLNVIRLRLPALRERSEDVPMLTRHFLQRSAAQLAV
                     APKRISDAALARLTRFAFPGNVRQLENICHWLTVMAPAQVISEQDLPPEVLPAGEAPP
                     DARAAPETPAGNSQGWESALEREAGQLLASGRSDVWDLLTRRFESRLITTALQATQGR
                     RMEAAQRLGIGRNTITRKIQELGLDAPSEE"
     gene            complement(5009..5785)
                     /gene="xth"
                     /locus_tag="PK518_10040"
     CDS             complement(5009..5785)
                     /gene="xth"
                     /locus_tag="PK518_10040"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRM49296.1"
                     /translation="MQIATWNVNSLSVRLPQVLAWLAANPVDALGLQELKLVDEKFPH
                     DAFEAAGYHAVCFGQKTYNGVAIVSRTPARDVVRNIPGFGDEQARVIAATLDTADGPL
                     RLVNCYFVNGQAPGTDKFAYKLEWLAALQDWLRDELAAHPRLALVGDFNIAPEDRDSY
                     DPVGLKDTIHHTVEERAHFQRLLDLGLTDAFRLFEQPEKSYSWWDYRMLGFQKNRGLR
                     IDHILVSQALRAGVTACHVDRAPRKNPQPSDHAPVVATLG"
     gene            complement(5896..6270)
                     /locus_tag="PK518_10045"
     CDS             complement(5896..6270)
                     /locus_tag="PK518_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRM49297.1"
                     /translation="MVQTTDYVATVFDGKSNPAKVTVTFTMALNAALKGHSATVILMV
                     EAVELGVPDATQGMDVGKPFEPVQQLLHKYLALGGRVAICSSCMIHNGFTAEQMDPRF
                     AIITAPEVIDLLMNAKGSLQIT"
     gene            complement(6307..6663)
                     /locus_tag="PK518_10050"
     CDS             complement(6307..6663)
                     /locus_tag="PK518_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5368 domain-containing protein"
                     /protein_id="HRM49298.1"
                     /translation="MKELDPLVFLAVFQEMLGPWLWVLLGLAALTTAAFIWLLLRQRG
                     IAPRRLVFAQLLGLAGGALALVLMAKVSSSGFTDAAGPADWILITLVFALGAIGATIV
                     TYTTLGWCRRSRKQAG"
     gene            complement(6677..7999)
                     /locus_tag="PK518_10055"
     CDS             complement(6677..7999)
                     /locus_tag="PK518_10055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRM49299.1"
                     /translation="MPLPSLQRRQLLALGAASAAATLASPAAQAQQPHKTQARIVIAG
                     AGAAGLAAASRLAALLQGARITLIDARREHFYQPGFTLVGGGIKPATYVTSSTADYLP
                     PGVELLTERVAEFDPDGNRVVTESGQAIAYDFLIVAVGLTLEYGLIEGMSPALIGKHG
                     IGSIYHSPAAAEATWQAMSAFADQGGRGVFLRPATEMKCAGAPLKYTFITEDHLTRRG
                     NRGKAELVYNSNNNALFSVPIVSEKVRMLYQERGIQVNYGRVLQSIDPGRRLATFKTA
                     EGSEEQPYDFIHVIPPMRAPEAVRSSPLPWTADKNKAWAAEGWLEVDKGTLRHLRYAN
                     VFGVGDIAGVPKGKTAASVKWQVPVALDHLVAEIAGKTSNALYTGYTSCPLITRLGRA
                     MLVEFDYQNNLVPSFPGAIAPLEELWVSWVMKTMALKPTYISMLRGRA"
     gene            8284..9333
                     /gene="lipA"
                     /locus_tag="PK518_10060"
     CDS             8284..9333
                     /gene="lipA"
                     /locus_tag="PK518_10060"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRM49300.1"
                     /translation="MSEPTIPLRPVPGAKFVSPQGTRAIKDGVKVSPITLVPDKHAKP
                     EWLRMKLPSGAKYQEVMEIVKSRQLSTVCAESKCPNISECWGRGTATLMLMGSVCTRA
                     CKFCSVSTGNPQGWLDHDEPAKVAEAVALMGLKYVVLTSVDRDDLADGGAAHYAACVR
                     AIHERMPDTAVEALTPDFQGRHDLVAQVLDAGLATYAQNLETVERLTHPVRDARAGYA
                     QTLAILKFAKGHAPQTITKTSLMLGLGESDEEIERALNDIRAANVDVVTMGQYMRPTK
                     NHLPVQRYVTPDEFQKYREYALGLGFVEAVAGPFVRSSYRAERVLEGNNLGLDAVNPE
                     VLEGIRDSAGASLRC"
     gene            complement(9338..9565)
                     /locus_tag="PK518_10065"
     CDS             complement(9338..9565)
                     /locus_tag="PK518_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49301.1"
                     /translation="MRSFETPDGQHWQAALLEASYGQVLLIFSTDAAGDLRQHSMPAE
                     HLAEATEQLAALDDAGLAALLTQAAPWQPGG"
     gene            complement(9579..10073)
                     /locus_tag="PK518_10070"
     CDS             complement(9579..10073)
                     /locus_tag="PK518_10070"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRM49302.1"
                     /translation="MEIALIYARAANGVIGHNGTMPWHLPEDLAHFKALTQGHPVIMG
                     RKTWDSLPARFRPLPGRTNIVITHQPDWHQNGVQRASSLGEALLFAEQTDTPTAWVMG
                     GAQIYAQALPLATRIEMTEIAQDFAGDAHAPTPGPEWVESARSSHVAASGLPYHFVTL
                     RRQG"
     gene            complement(10107..>11966)
                     /gene="fusA"
                     /locus_tag="PK518_10075"
     CDS             complement(10107..>11966)
                     /gene="fusA"
                     /locus_tag="PK518_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRM49303.1"
                     /translation="SLQASLMHAAHGGCRIHLIDTPGLPDFIGQSLAALEAVETAAIV
                     ISAAAGIEPMARRMMEYAGQRQLDRLIIVNKIDAEGADLPALLADIQATFGKECLPLN
                     LPDAGATRVVDCFYNRAGHSDFGAVADAHRALVEQVVEVDGAFVDRYLNDGDVDASEL
                     HAPLEQALREGHLIPVCFVSARTGAGVAELLDVIARLLPNPGEGNPPDFLNGEGDAAR
                     LMHARPDPAAHVLAHVFKVSIDPYVGKLGFVRVHQGTIGPATQLYVGDGRKPFKVGHL
                     YRRQGKNQTEVASAGPGDICVIGKVDELQFDAVLHDAAEDDHIHLKPLPFPVPVHGIA
                     LLPKRRGDEQRMWEIMNKLVAEDPCLLIEHVPATNETIARGLGELHLRVLLDRLREVY
                     KFEVDTRAPRIAYRETITHGAAAQYRHKKQSGGAGQFGEVHLRVEPLARGAGFEFVDQ
                     VKGGTIPGQFMPAVEKGVREALAEGVIAGFPVQDLRVIVYDGKHHAVDSKEVAFVTAG
                     KRAFQAAIREAAAVVLEPIARLSVSAPESAMGAITGDLSARRGIVSGTDSDRQGQLTI
                     LGEAPLAELADYQNRLNAMTAGQGSYTLALSHYEAVPPAVQQQLVGQYKVQEE"
BASE COUNT         1979 a         3982 c         4022 g         1983 t
ORIGIN      
        1 ctgcccagcg tcgatcaccc ggtgcatggc aggcatgtcg agttgggcgt gaaaggcgcc
       61 gcagccctgg tgcctgccgc gtggcaggag atgcaccagg cattgcacca gtttggtgca
      121 gtgatgggcc ccgaaacggt gcatcatttg taatcttaga atcacgcgcc ttgcttgcct
      181 gggtgctgca gcgctggcac gcaagctgct taccaatgtt catctttgtg gatgagtcct
      241 ttatttttgg agaaacctga tggccaagac cgttgcagac gtgatgaaga tggtggagga
      301 gaacgaagtc aagttcgtgg acttgcgctt caccgatacg cgcggcaagg agcagcacgt
      361 gacggtgcct gtttctcact tcgatgaaga caagttcagt tccggccatg cctttgatgg
      421 ttcgtcggtc gccggctgga agggtatcga ggcctcggac atgcagctca tgcccgatcc
      481 gagcacggcc aacatcgacc cgtttttcga ggagaccacg ctgtttctgc agtgcgacgt
      541 ggtggagccc ggcgacggca aggcctacga ccgcgacccg cgctccatcg ccaagcgcgc
      601 cgaggcctac ctgaaggcct ccggtctggg cgacacggcc tacttcggcc ccgagcccga
      661 gttcttcatc ttcgacggcg tgcgctggag caacgaaccg ggcaaggtgt tcttcgagat
      721 cgaggaatac gaagccccct ggaacagcgg caccaagttc gaaagcggca accgcggcca
      781 ccgccccacc accaagggcg ggtacttccc cgtgccgccg gtggacagca cgcaggacat
      841 gcgcgccgag atggtgctga tcctcgagca actgggcatt ccggttgaag tcttccacca
      901 cgaggtggcc ggcgctggtc agaacgagat cggcacgcgc ttctccaccc tggtcgagcg
      961 cgccgactgg acccaggtgc tgaagtatgt ggtgtggaac gtggccaacg cctacggcaa
     1021 gacggctacc ttcatgccca agccctatgc cggcgacaac ggatcgggca tgcatgtgca
     1081 ccagtccatc tggaaggacg gtaaaaacct gttcgcgggt gacggctacg ccggcctgtc
     1141 ggattttgcg ctgtactaca tcggcggcat catccaccac gcccgcgcgc tgaacgccat
     1201 caccaacccc ggcaccaaca gctacaagcg cctggtgccc ggctacgagg caccggtgaa
     1261 gctggcctac agctcgcgca accgctcggc ctcgatccgc atcccctatg tggccaaccc
     1321 caagggccgc cgcatcgagg cgcgcttccc cgatcccctg gccaacccct atctgtgctt
     1381 ctcggcactg atgatggccg gcctggacgg cgtggagaac aagatccatc cgggcgaggc
     1441 cgccaccaag gatctgtacc acctgccgcc cgaagaggac aaactggtgc ccaccgtgtg
     1501 ccatagcctg gatcaggcgc tggaggcgct ggacaaggat cgcggcttcc tgaccaaggg
     1561 cggcgtgttc tccgacaaca tgctggacgc ctacatcgag ctgaagatgg gcgaggtgca
     1621 gcgcttccgc atggcgccgc acccggttga gtacgacatg tactactcgc tgtaagtcgc
     1681 agcggggctt gcggcccatg gcaccaaggc ggctgcggcc gccttggtcg tttctggatg
     1741 atggctggca acagccggaa catttggcgc catctcgggt cttttgagct gatcgcatgg
     1801 cggcacaatg ggtgccatgc ggtgattgct ggatactcgc cgccatcgtg catgcttggc
     1861 gcatgccctg taaggatctc gatgaaacac tgcctgttcg ccctgaccgt cattgccgcc
     1921 atgctgcagc ctgcctgggc gcaggatcgc atctaccgtt gcggcaacga gtacaccaac
     1981 aacgccgccc aggccaagga gcgcggctgc aagctggtcg agggcggcaa cgtcacggtg
     2041 gtcgagggca cgcgccccgc agccgctccc tcgggtggtg ccgccgcatc atcggccagc
     2101 aaggccgcag cctcgccccc gaatgcgccc cgcgtcgcgg cgaccgaaca gcgcgctcgc
     2161 gacgccgatg cgcgcgccat tctgcaggat gagctgcgca aggccgaaag ccgcctggcc
     2221 gatctgcgca aggagtacaa cgacggcgcg ccgcagcgct cggccctgga actgcgcaac
     2281 ccgcagggtt atatcgagcg caccgccgag ctcaaggctg ccgtcacccg cgccgaggcc
     2341 gatgtggccg gcatcaagcg cgaactgggg cgtctgggaa attaagcgcc caggggcacc
     2401 gtcattccag cgccacccgc accaccgcgc taccaggcgc tggatctgct gtccacgctc
     2461 gtagccgtgc tggatgcgca gggcagggtg ctgtatgtga atgccgccct ggaaaacgcc
     2521 ctgggcctgt cgcgccggat gctgctgggt gctgactttg ctgcgctgtt tggcgacccg
     2581 gccaccgtgc agaccgccct ggccgcggtg cgcgcccagg actatgccca gatgcgctgc
     2641 gaggccgcgt tgcgtcgccc gcagcaagaa cccttgccgg tgcatgcgca tatcgccccg
     2701 gccgaacacg ctggagaaca ggtggtcgag ctgtggccgc ttctggccca ggcgcaccag
     2761 gacagggaag agcgcctcct ggagcaggcc caggccaaca aagagctgat acgcaacctg
     2821 gcgcatgaga tcaagaaccc gctgggcggc atacgcggcg cggcccagtt actgcagctg
     2881 gagctggcca gcccggagct tgccgaatac acccaggtca tcatccacga ggccgatcgg
     2941 ctgcagcatc tggtggacag gctgctggca ccgcaccgcc agccgcgcct ggtgggcgat
     3001 gtgaatatcc acgaggtctg cgagcatgtc tgccgcctgg tgctggccga gcacccgcgc
     3061 ggcctggtga tccagcgcga ctacgacacc tccatccccg agttccgcgg cgaccgcgcg
     3121 cagctcgtcc aggcgctgct gaacatcgtg cagaacgccg cccaggcgct gggcgagcgc
     3181 atcgccgccg gtgacgcgca aatcaccctg cgcagccgcg tcgcgcgcca ggtcacgctg
     3241 gggcgccagc gccacaggct ggcattggaa ttgcatgtca tcgacaacgg cccgggcgtg
     3301 cccgaggcca tcaaggagcg catcttttat cccctggtga ccggccgggg tggcggttcg
     3361 ggcctgggcc tgccgctggc gcaggccttt gtgcagcacc accagggcat gatcgactgc
     3421 gagagccggc cgggttgcac agactttcgc atcctcattc cattgccatg actagtcata
     3481 gaaacccaca cgcatgaagc cgatctggat cgtggacgac gacgcctcca tccgcttcgt
     3541 gttggaaaag gcgctggcgc gcgaacacct gcccacgcgc agcttcaccc agccgcgcga
     3601 ggtgctggac gctctggccg aggccggcga gccgggcccg gcggcgctgg tcagcgacat
     3661 acgcatgccc ggcggctcgg gcctgcagct cttggagcag ctgcgcgcgc gcctgcccgg
     3721 cctgccggtg atcatcatca ccgcctattc cgacctggac agcgccgtct cggcctacca
     3781 gggcggcgcc tttgaatacc tgcccaagcc gtttgacgtg cccaaggcgg tggagctgat
     3841 ccgccgcgcc gtgcaggaaa gccagcgcga ggaggtggcc gagcagcagc tggccgccac
     3901 ccccgagatg ctgggccagg ccggcgccat gcaggacgtg ttccgcgcca tcggccggct
     3961 gagccagagc gcggtcacgg tgctcatcac cggcgaatcg ggcaccggca aggagctggt
     4021 ggcgcgtgcg ctgcacaagc attcgccggt ggcgggcggg cctttcgtgg ccatcaacac
     4081 cgcagcgatt ccgaaggacc tgctggaaag cgaactcttc ggccacgagc gcggcgcctt
     4141 caccggcgcg caggcgcagc ggcgcgggcg cttcgaacag gccgagggcg gcaccttgtt
     4201 cctcgacgaa attggcgaca tgccgtttga cctgcagacg cggctgctgc gcgtgctctc
     4261 ggatggccag ttctaccgcg tgggcggcca tgccccggtc aaggcccatg tgcgcgtgat
     4321 tgccgccacg caccagaacc tggagcagcg cgtcaaggac ggcagctttc gcgaggattt
     4381 gctgcaccgc ctgaacgtga tccgcctgcg cctgcctgcg ctgcgcgagc gcagcgagga
     4441 cgtgcccatg ctcacgcgtc acttcttgca gcgcagcgcg gcgcagctgg cggtggcgcc
     4501 caagcgcatc tcggacgcgg cgctggcgcg cctgacgcgc tttgcctttc ccggcaacgt
     4561 gcgccagctg gagaacatct gccactggct caccgtgatg gcgccggcgc aggtcatctc
     4621 cgagcaggat ctgccgcccg aggtgctgcc ggccggcgaa gcgccgccgg acgcgcgcgc
     4681 agccccggaa acgccagccg gcaacagcca gggctgggag agcgcgttgg agcgcgaggc
     4741 cggccagctg ctggccagcg gccgcagcga tgtgtgggat ctgctcaccc gccgcttcga
     4801 gtcgcgcctg atcaccaccg ccctgcaggc cacgcagggc cggcgcatgg aggccgcgca
     4861 gcgcctgggc ataggccgca acaccatcac gcgcaagatc caggagcttg gtctggatgc
     4921 cccaagtgag gagtaaaaat ggttgtggcg ccctattggt gggcgtcaac agctatataa
     4981 aatgtagtga ttggtgccct gttctgcttc agcccagcgt ggctaccacg ggcgcatggt
     5041 cgctcggttg ggggttcttg cgcggcgcgc ggtccacatg gcaggcggtc acaccggcgc
     5101 gcagggcctg gctgaccagg atatggtcga tgcgcaggcc gcggttcttc tgaaagccca
     5161 gcatgcggta gtcccaccat gagtagctct tctcgggctg ctcgaacagg cggaaggcat
     5221 ccgtcaggcc caggtccagc agccgctgga aatgcgcgcg ttcctccacc gtgtggtgaa
     5281 tcgtgtcctt caggcccacc gggtcgtagg agtcgcgatc ctcgggcgcg atgttgaagt
     5341 cgcccaccag cgccaggcgc gggtgcgctg ccagctcgtc gcgcagccag tcctgcagcg
     5401 cggccagcca ctcaagcttg taggcaaact tgtccgtgcc cggcgcctgt ccgttgacaa
     5461 agtaacagtt gaccaggcgc agcgggccat ctgcggtatc cagcgtggcg gcgatgacac
     5521 gcgcctgctc gtcgccaaac ccggggatgt tgcgcaccac gtcgcgcgcc ggcgtgcggc
     5581 tgacgatggc cacgccgttg taggtcttct ggccaaagca gacggcgtgg tagccggcgg
     5641 cctcgaaggc gtcgtgcgga aacttctcgt ccaccagctt gagctcctgc aggcccaggg
     5701 catcgacggg gttggcggcc agccaggcca gcacctgggg caggcgcacg gatagggagt
     5761 tgacgttcca ggtggcgatt tgcatggttc ttgagagcag tggctgtggg gccggggctt
     5821 cagaaccatc tggcggcatg cgtggtgcat gccgggcccc tggcgcgggg cccgattatg
     5881 ggcgatgcac gacgctcagg tgatttgcaa cgatcccttg gcgttcatca gcaggtcgat
     5941 gacttcgggc gcggtgatga tggcaaagcg cggatccatt tgctcggcgg taaagccgtt
     6001 gtggatcatg cacgagctac agatggccac gcgcccgccc agcgctaggt atttgtgcag
     6061 cagctgctgc acgggctcga agggcttgcc cacgtccatg ccctgggtgg cgtcgggaac
     6121 gcccaactcc acggcctcga ccatcaggat gacggtggcg ctgtggccct tgagggcggc
     6181 gttcagcgcc atggtgaagg tcacggtgac cttggcgggg ttgctcttgc catcaaaaac
     6241 ggtggccacg tagtcggttg tctgcaccat gggtagttcc ttgatgtgga ggaaagtggg
     6301 ggtatttcag cccgcttgct tgcggctgcg cctgcaccag ccgagggtgg tgtaggtgac
     6361 gatggtcgca ccaatggcac ccagcgcaaa caccagggtg atcagtatcc agtcggccgg
     6421 ccctgcggcg tcggtaaagc ccgaggacga caccttggcc atcagcacca gggccagggc
     6481 gccgcctgcc aggcccagca gctgcgcgaa caccagccgc cgcggcgcaa tgccgcgctg
     6541 gcgcagcagc agccagatga aggccgccgt ggtcaacgcg gccaggccca gcagcaccca
     6601 cagccagggg ccgagcatct cctggaacac ggccaggaac accaggggat cgagttcttt
     6661 catggtgacg ctcctttcag gcgcggccgc gcagcatgct gatataggtg ggcttgaggg
     6721 ccatggtctt catcacccag ctcacccaca gctcttccag cggcgcaatg gcgccgggga
     6781 atgacggcac caggttgttc tggtagtcaa actcgaccag catggcccga cccagccgcg
     6841 tgatcagcgg gcaggaggtg tagccggtgt acagggcatt cgaggtcttg ccggcaatct
     6901 cggccaccag atgatccagc gccaccggca cctgccactt cacgctggcg gcggtcttgc
     6961 ccttgggtac accggcaatg tcgcccacgc cgaacacgtt ggcgtaacgc agatggcgca
     7021 gcgtgccctt gtccacctcc agccagccct ctgccgccca ggccttgttc ttgtcggccg
     7081 tccagggcag ggggctgctc cgcacggcct cgggcgcgcg catgggcggg atgacgtgga
     7141 tgaagtcata gggctgctcc tcgctgccct cggccgtctt gaaggtggcc aggcgccggc
     7201 ccgggtctat ggactggagc acgcgcccgt agttcacctg tatgccgcgc tcctggtaga
     7261 gcatgcgcac cttctcggag acgatgggca cgctgaagag tgcgttgttg ttcgagttat
     7321 agaccagctc ggccttgccg cggttgccgc gtcgcgtcag gtgatcctcg gtgatgaagg
     7381 tgtatttgag cggtgctccc gcgcatttca tctcggtggc cgggcgcagg aacacgccgc
     7441 gcccgccctg gtcggcaaag gccgacatcg cctgccaggt ggcctcggcc gctgccgggc
     7501 tgtggtagat gctgccaatg ccgtgcttgc cgatcagcgc cgggctcatg ccctcgatca
     7561 ggccgtactc tagcgtcagg cccacggcga cgatcagaaa gtcgtaggcg atggcctgcc
     7621 cgctctcggt caccacgcgg ttgccatcgg ggtcgaactc ggctacgcgc tcggtcagca
     7681 gctccacgcc tggtggcaga tagtccgcgg tgctggaggt gacataggtg gccggcttga
     7741 tgcccccacc caccagggtg aagcccggtt ggtagaaatg ctcgcgacgc gcgtcgatca
     7801 gggtgatgcg cgcgccctgt agcagcgccg ccaggcgcga tgcagcggcc agtcccgccg
     7861 cgcccgcacc cgcgatcacg atgcgtgcct gggttttgtg tggctgctgg gcctgggcgg
     7921 cggggctggc cagcgtggcg gcagcgctgg cggcacccag tgccagcagc tggcggcgtt
     7981 gcagggatgg cagtggcatg gcagtacctc cgatgattct aaatttagaa caatctaata
     8041 tttgtcatgg tagtgcattc agggataaat ggaaacagga tgttggtgat ccgacatccc
     8101 ctcgccgggc aggcctttgg cgggcgcacg aagtgccgcc catgggccct ggatcaaggc
     8161 gtgaattgcc ccaagcccga taatgctgcc cttgccccgg ccggcatggc ctggcccggg
     8221 tgccgttttc atcacgatcg ccgccgtgac cggcgctatc cacggtgcaa ggaccgtcag
     8281 accatgagtg agcccaccat tcccctgcgc cccgtaccgg gcgccaagtt tgtcagcccg
     8341 cagggcacgc gcgccatcaa ggatggcgtg aaggtcagcc ccatcaccct ggtgcccgac
     8401 aagcatgcca agcccgaatg gctgcgcatg aagctgccct cgggcgccaa gtaccaggag
     8461 gtgatggaga tcgtgaagag ccgccagctg tccaccgtct gcgccgagtc caaatgcccc
     8521 aacatctccg aatgctgggg ccgcggcacg gccacgctga tgctgatggg ctcggtgtgc
     8581 acgcgcgcct gcaagttctg ctcggtcagc accggcaacc cgcagggctg gctggaccat
     8641 gacgaacccg ccaaggtggc cgaggccgtg gcgctgatgg gcctgaaata cgtggtgttg
     8701 acctcggtgg accgcgatga cctggctgac ggcggcgccg cgcactacgc ggcctgcgtg
     8761 cgtgccatcc acgagcgcat gcccgacacc gcggtggagg cgctgacgcc cgacttccag
     8821 ggccgccacg acctcgtggc ccaggtgctg gacgcgggcc tggccaccta cgcgcagaac
     8881 ctggagaccg tggagcgcct gacgcacccg gtacgcgacg cgcgcgcggg ctacgcgcag
     8941 acgctcgcca tcctgaagtt tgccaagggc cacgcgccgc agaccatcac caagaccagc
     9001 ctgatgctgg gcctgggcga gagcgacgag gagatcgagc gcgcgctgaa cgacatccgc
     9061 gccgccaacg tggacgtggt gaccatgggc cagtacatgc gcccgaccaa gaaccacctg
     9121 ccggtgcagc gttacgtgac gcccgacgag ttccagaagt accgcgagta cgccctgggc
     9181 ctgggcttcg tcgaggccgt ggccggcccc ttcgtgcgct ccagctaccg cgccgaacgc
     9241 gtgctggagg gcaacaacct ggggctggat gcggtcaacc ccgaggtgct ggaaggcata
     9301 cgcgactcgg ccggcgcgtc gctgcgctgc taaggcgcta acccccgggc tgccagggcg
     9361 cggcctgggt cagcagggcg gccagcccgg catcgtccag cgccgccagc tgttcggtgg
     9421 cctcggccag gtgttcggcc ggcatgctgt gctggcgcag atcgcccgca gcgtcggtgc
     9481 tgaagatcag cagcacctgg ccgtaggagg cctccagcag cgcggcctgc cagtgctggc
     9541 cgtcgggggt ttcaaagctg cgcatcgctc ccccgtattc agccctgccg gcgcagggtg
     9601 acgaaatggt atggcaggcc gctggccgcc acatggctgc tgcgcgccga ctccacccat
     9661 tcggggccgg gtgtgggtgc gtgggcgtcg ccggcaaagt cctgtgcgat ttcagtcatc
     9721 tcgatgcgcg tggccagcgg cagggcctgg gcgtagatct gcgcgccacc catgacccat
     9781 gccgttggcg tatcagtttg ctctgcaaat aatagtgctt cgcccagact ggatgcgcgc
     9841 tgtacgccat tttgatgcca atctggttgg tgtgtgatga cgatgttggt gcgccccggc
     9901 aacgggcgaa agcgcgccgg cagcgaatcc caggtcttgc gccccatgat caccgggtgg
     9961 ccctgggtga gcgccttgaa atgcgccaga tcctccggca ggtgccaggg catggtgccg
    10021 ttgtggccga tcacgccatt ggcggcgcgg gcatagatga gagcgatttc catggtgcgg
    10081 cgagcataac gccggcggca cagccgtcat tcctcctgca ccttgtactg ccccaccagc
    10141 tgttgctgca ccgcgggcgg caccgcctcg tagtgcgaca gggcgagcgt gtagctgccc
    10201 tggccggcgg tcatggcgtt cagccggttc tggtagtcgg ccagctcggc caggggcgct
    10261 tcgccaagga tggtcagctg gccctgccgg tcgctgtcgg tgccgctgac gatgccgcgg
    10321 cgcgccgaca ggtcgccggt gatggcgccc atggcgctct ccggggcgct gaccgaaagg
    10381 cgcgcaatcg gctccagcac caccgcggcg gcctcgcgta tcgccgcctg gaaggcgcgc
    10441 ttgccggcgg tgacaaaggc cacctccttg ctgtccaccg cgtggtgctt gccgtcgtag
    10501 acgatgacgc gcaagtcctg taccggaaag ccggcgatca cgccctcggc cagcgcctca
    10561 cgcacgcctt tctcgaccgc cggcatgaac tggcccggaa tcgtgccgcc cttgacctgg
    10621 tccacgaact cgaagcccgc gccgcgtgcc aaaggctcga cgcgcagatg cacctcgcca
    10681 aactggccgg cgccgccgct ttgcttcttg tggcggtatt gcgccgccgc gccgtgggtg
    10741 atggtctcgc ggtaggcgat gcgcggggcg cgcgtgtcca cctcgaactt gtagacctcg
    10801 cgcaggcgat ccagcagcac gcgcaggtgc agctcaccca gtccgcgggc tatggtttcg
    10861 ttggtggccg gcacatgctc gatcagcagg caggggtctt cggccaccag cttgttcatg
    10921 atctcccaca tgcgctgctc gtcgccacgg cgcttgggaa gcagggcgat gccgtgcact
    10981 ggcacgggga aggggagggg tttcaaatgg atatggtcgt cctctgcggc gtcgtgtagc
    11041 acggcgtcga actgcagctc gtccaccttg ccaatgacgc agatgtcgcc cggcccggcg
    11101 ctggccacct cggtctggtt cttgccctgg cgccggtaca ggtggcccac cttgaacggt
    11161 ttgcgcccgt cgcccacgta cagctgcgtg gccggcccta tcgtgccctg gtgcacacgc
    11221 acaaagccca gcttgccgac ataggggtca atgctcacct tgaacacatg ggccagcaca
    11281 tgggccgccg ggtcgggcct ggcgtgcatc agcctggcgg cgtcgccctc gccgttcaga
    11341 aagtcgggcg gattgccctc gccggggttg ggcagcagcc gggcgatcac gtccagcagc
    11401 tcggccacgc ccgcgcccgt gcgcgccgag acaaagcaca ccgggatcag atgtccctcg
    11461 cgcagcgcct gctccagcgg cgcgtgcagc tcgctggcat ccacgtcgcc gtcgttcagg
    11521 tagcgatcga cgaaggcgcc atccacctcc accacctgct cgaccagcgc gcggtgggcg
    11581 tccgccacag cgccaaagtc gctgtggccg gcgcggttgt agaagcagtc caccacccgc
    11641 gtcgcgcccg cgtcgggcag gttcaaaggc aggcattcct tgccaaacgt ggcctgaatg
    11701 tcggccagca gggcgggcag gtcggcgccc tcggcgtcga tcttgttgac gatgatcagg
    11761 cggtcgagct gccgctgccc ggcgtactcc atcatgcgcc gcgccatggg ctctatgccc
    11821 gcggcggcgc tgatgacgat ggccgcggtc tccaccgcct ccagcgccgc caggctctgg
    11881 cctatgaagt cgggcaggcc gggggtgtcg atcaaatgga tgcggcagcc gccatgcgcg
    11941 gcatgcatga gcgaggcctg cagcga
//
LOCUS       DAOMTQ010000095        11930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_983_length_11930_cov_27.834695, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000095 DAOMTQ010000000
VERSION     DAOMTQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_983_length_11930_cov_27.834695"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..2639)
                     /locus_tag="PK518_10080"
     CDS             complement(159..2639)
                     /locus_tag="PK518_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM49304.1"
                     /translation="MGRQPFQAIWDGLALAAWLVDAKSLQILQANEAASLLVGRRVAD
                     MEGMPILELAATPEDQIFWGQPTTDIAAGIHSHTWVRNMRSQALVPVDRRVLAIHAGA
                     NAGAAQCLLLTMQDRSEHEASRLELEALLSELRATLDSAVDGVLTCNLNGQMRAFNHR
                     LVEIWQLPHEMLLQRDDAAIWTHIESLVLDSEVYRAGRAEIVREPMRESHDILLLRNG
                     MTVEQRSVPQLVQGRPAGRVYTFRDITSDIQNQADLRLAARVFESSLDAVFIADRRNA
                     ILRINPACEQLLRISADGGVIGQSVTTLFAKGKGRSAFLSEVQQGWLQNGFWEGELHL
                     RQRDGGDCSVHLSWVVLRDAEGGDAQSIGFMRDLTQQHAAKRRIEQLAYRDALTGLPN
                     GLLLSERVTAAIELSRATGDSFAILFLDLDRFKIVNDSLGHPFGDRVLKLVAERLQGC
                     LRQADMLCRLGGDEFVIYLHGADVGIAEGVARRMLDELQRPFMLDGMGFSIQCSIGVS
                     MFPRDGESLDDLIKQADTAMYQVKERGRGNFGFYRPQMNANLLSRMKLEHAMRQALGL
                     GHMAVHYQPQVDMATGRIVGAEALARWTDPEFGVVSPGSFIPLAEESGFIVSLGGWVM
                     EQAIKEAAHWMSCGTPLVVSVNVSALEFRQPGFVERITALLTRYGLPAQQLELELTES
                     VLLHDAQEMEQVLASLTRQGLRLSIDDFGTGYSSLAYLKKLAIHRLKIDRTFICGLPD
                     DEGDSAIVKAVLGMGRALGFSVVAEGVETQEQRLALHTMGCEYYQGFLCAPALSAAEF
                     RSRVLGPSAVEQKHQSKRTPCRSRAAKR"
     gene            complement(2642..3907)
                     /locus_tag="PK518_10085"
     CDS             complement(2642..3907)
                     /locus_tag="PK518_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HRM49305.1"
                     /translation="MNQAVLIDIDALRVGMFIQLELGWINHPFPMSSFRLSSPDQIRV
                     LREIGLQSVRYIPAKSTVTAANAGLRSEEARDEPESDAMVQAELGAADLLAQFNARQQ
                     HCNLRFQEATRTYAAVSAHVRAEPAQAREQAEALIRTCVSDLLTRGPCAVHLLADGVS
                     QRSAAHAVNVMVLALLLGQAIGLQAQQLHGLALSALLHDLGKVNLPAHIGEPGAALRA
                     ADMQRYHSHVGLSVELAQNMALPSDVLIAIAQHHEMADGSGYPLHLVADDISRWGQIL
                     ALVNRYDRLCNPLHGDQALTPHEAVAKLFAQLRGSFDASVLGAFIRLMGVYPPGSLVQ
                     LVDGRLAVVMVVDPARPLRPWVVPHQPDVPRSQVALLNLAQTPELGILRSLKPAQLPR
                     AALEYLLPQPRICYFFARALDSLGEGDAA"
     gene            complement(4013..4735)
                     /locus_tag="PK518_10090"
     CDS             complement(4013..4735)
                     /locus_tag="PK518_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRM49306.1"
                     /translation="MLWALASTAFFMGLMGGTHCLVMCAAPCAALTGKGAAPAGDTRV
                     VRLAPQAGTLQRTAGFHLGRLMGYGFAGALAAWAMDRLAWLGQNTAALRPAWTLLHVL
                     ILAWGLLMMLQARQPAWLEHAGRSVWTKVRALVARPGGLFVTGMAWALLPCGLLYTAL
                     LTAALSGSVLRGALCMVLFGVGSGVWLVAGPILWRSFRARVNRVASSWGSRLAGVVLF
                     AMGGTALWMDVVHGQPAPWCLP"
     gene            complement(4739..6121)
                     /gene="hemN"
                     /locus_tag="PK518_10095"
     CDS             complement(4739..6121)
                     /gene="hemN"
                     /locus_tag="PK518_10095"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRM49307.1"
                     /translation="MTVVTTELLRRFDVPGPRYTSYPTADRFVEAFGEKEYILALQQR
                     RVGSVAKAMPLSLYIHIPFCESLCYYCACNKIITRHPERADVYLRYLSREIDLHTAHC
                     GVGQHVSQLHFGGGTPTFLSDDGLRELMGMLKRSFVLVPGGEYSIEVDPRTVDEQRLA
                     LLAELGFNRLSFGVQDFDPEVQKAVHRIQPAEKVFGLVESARKLGFDSVNVDLIYGLP
                     RQTPESFDRTLEQVAQLRPDRIALYAYAHLPERFKSQRRIITAELPMASAKVSMLARS
                     IDAFMNAGYVYVGMDHFALPEDALAVAKRQGRLHRNFQGYSTQPDCDLIGLGVSAIGR
                     VGATYSQNVKTLDEYYDAIDHGHLPVVRGLALTKDDLVRRAVIMALMCQGELLFEPLE
                     QSWLIDFRSYFEAELRQLEEMAEQGLVSISPEGVKVTTMGWFFVRGVAMVFDRYLQAD
                     RNRARFSRII"
     gene            6293..7036
                     /gene="fnr"
                     /locus_tag="PK518_10100"
     CDS             6293..7036
                     /gene="fnr"
                     /locus_tag="PK518_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HRM49308.1"
                     /translation="MNRVSMSSNLQTIKVACSNCNLRELCMPVGLDDQQLKRIDDIVA
                     VRRKVKRGGTLFRNGEAFTSLFAIRTGFFKTCVATEDGRDQVTGFQMAGEIIGLDGIV
                     NDRHTCDAVALEDAEVCVMPFERIEELSRVVTALQTHVHKIMSREIVREHGVMLLLGS
                     MRAEERLAAFLLNLVQRLHARGFSQSELVLRMTREEIGSYLGLKLETVSRTFSKFVEE
                     GIVEVKQRHVRIVNTEALKRIVNSQQACH"
     gene            complement(7071..7358)
                     /locus_tag="PK518_10105"
     CDS             complement(7071..7358)
                     /locus_tag="PK518_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49309.1"
                     /translation="MWMQRLMWIAWPAFLMAGVMEMVVFAFVDPEALQWFDQPVNLSR
                     EGVYTVAFFLFWLVITISGALTTLLSFSPFEFNHRESSETDRPADNPKYVS"
     gene            complement(7442..7720)
                     /locus_tag="PK518_10110"
     CDS             complement(7442..7720)
                     /locus_tag="PK518_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRM49310.1"
                     /translation="MSPNSSANSTPESQPWWKYGHVWLIIAGPVIVVIAGFITLWLAL
                     ARPDPVLTEDYYQRGLDINKTLQNSQMSMTPATKGRNHAATPVQDQPR"
     gene            complement(7800..9242)
                     /gene="ccoG"
                     /locus_tag="PK518_10115"
     CDS             complement(7800..9242)
                     /gene="ccoG"
                     /locus_tag="PK518_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRM49311.1"
                     /translation="MKPADGKPRKVIPIAAAPAEKPKTESASLYEAQKKVYPRSISGV
                     FANWRWIMVYVTQLIFYGLPWLQWGERQMVLFDLGARRFYIFGLVLYPQDFIYLTGLL
                     IISALALFLFTAVAGRLWCGFSCPQTVYTEIFMWIEHKIEGDRSARIRLDNGPWTFEK
                     TWKKSLKQLVWITVSFWTGFTFVGYFVPIRELGGELMSLHGSWQIFWVFFYGLATYGN
                     AGYLREQVCKYMCPYARFQSAMFDNDTLVVTYDPERGEPRGPRTKAVDYKAKGLGDCI
                     DCSLCVQVCPTGIDIRDGLQYDCIGCGLCVDACNTVMDKMQYPRGLIRFSTQNGVKNH
                     WTQSQMLKRVLRPRVLFYSGILIALCIGMMASLVVRTPLKVDIVRDRAALSRIVAGGK
                     LENIYRLQIMNATEGAQRYVISARGLDGLEVISEPEIDIGPAESRWVVVRLQIPYGSA
                     APGSHTVYFDVRAQDDKGQVAEKSVFLVPR"
     gene            complement(9328..10242)
                     /gene="ccoP"
                     /locus_tag="PK518_10120"
     CDS             complement(9328..10242)
                     /gene="ccoP"
                     /locus_tag="PK518_10120"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRM49312.1"
                     /translation="MSDFTSNFWSIYVAGITLAGIIACALLLWLTSRKKIESAADNTT
                     GHVWDVDLREMNNPMPRWWMWLFVITIVFAFAYLAAYPGLGTFKGQLGWTQVGEYQAE
                     VDKANAELQPLYARFDSMKTEDIAADQAAMAIGERLFMNNCAQCHGSDARGSKSFPNL
                     SDKDWLHGGTPDDIRKTIHDGRVGVMPPMAAAVGSPDDVRNLAQYVLSLSGSPHDSLK
                     ASLGKAKFTACAACHGMDGKGMTAMGAPNLTDDIWLHGWGEAAIVAMINDGKTNQMPA
                     QAGKLTDQQINVLTAYVWGMSNKPGAAK"
     gene            complement(10272..10412)
                     /locus_tag="PK518_10125"
     CDS             complement(10272..10412)
                     /locus_tag="PK518_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRM49313.1"
                     /translation="MDITTMRIVVTLASLACFLGIWYWAYTARNRAQFDEAAQLPFAN
                     DD"
     gene            complement(10425..11057)
                     /gene="ccoO"
                     /locus_tag="PK518_10130"
     CDS             complement(10425..11057)
                     /gene="ccoO"
                     /locus_tag="PK518_10130"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRM49314.1"
                     /translation="MSNTNKQTSSGFTHEKVETSNFLMIVLILVVIAMGGLVEIVPLF
                     FQKSTTEPVQGLKPYTALQLAGRDIYQREGCYNCHSQMIRPFRAETLRYGHYSVAGEF
                     VYDYPFQWGSKRTGPDLHRVGGKYSDEWHRIHLVNPRDVVPESNMPAYPWLEKNQVDA
                     ASMAPHMRALRRVGVPYTDEEIAGSAEAVKGKTEMEAVIAYLQVLGTALK"
     gene            complement(11076..>11930)
                     /locus_tag="PK518_10135"
     CDS             complement(11076..>11930)
                     /locus_tag="PK518_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRM49315.1"
                     /translation="AGVQDAMVQWWYGHNAVGFFLTTGFLGMMYYYIPKQAGRPVYSY
                     RLSIVHFWALIFTYMWAGPHHLHYTALPDWTQSVGMVFSLILLAPSWGGMINGIMTLS
                     GAWHKLRDDPILRFLIVSLSFYGMSTFEGPMMSIKTVNALSHYTDWTVGHVHSGALGW
                     VGLVTMGSLYYMIPRLFGREKMHSIKAIEIHFWLATIGIVLYIAAMWIAGVMQGLMWR
                     AVNPDGTLTYTFVESVKATYPFYAIRFLGGVLYLAGMCFMAWNTWMTAISGRSVKVAI
                     PAVNPAHA"
BASE COUNT         2681 a         3755 c         3286 g         2208 t
ORIGIN      
        1 tgagcacggg cttggtacat tgcctggtga gcgtgatcgt ctcgcgtcag atgaacccaa
       61 cgccaggctg ccctcgccca cctgaacccc cggttcagga tgacggcccg gtggcatttg
      121 cagacacctt tttttgcagc ccgtgcggat cccagtcttc agcgcttggc agcgcgcgag
      181 cggcagggcg ttcttttgga ctggtgcttt tgttccacgg ctgatgggcc aagaacgcgg
      241 gagcgaaatt ccgcggcgct cagggctggc gcgcacaaga aaccctggta gtactcacag
      301 cccatggtgt gcagcgcaag gcgctgttcc tgcgtctcca cgccttccgc caccacgctg
      361 aaacccaggg cccggcccat gcctagaacc gccttgacga tggcgctgtc accctcgtca
      421 tcaggcaggc cacagataaa tgtccgatcg attttcagcc ggtgaatggc cagcttcttc
      481 aggtaggcca ggctggagta tccggtgcca aaatcatcga tggagagcct gagtccctgg
      541 cgcgtcaggc ttgccagcac ctgctccatt tcctgggcat cgtgcagcaa cacgctctcc
      601 gtcagttcca gctccaactg ctgggccggc aggccataac gggtcagcaa ggcggtgatg
      661 cgctcgacaa aacccggctg ccgaaactcg agcgcggaga cattgaccga caccaccaag
      721 ggcgtgccgc agctcatcca gtgcgccgcc tccttgatgg cctgctccat cacccagcca
      781 cccagactga cgatgaaacc ggactcctcc gccagcggaa taaagctgcc cggcgacacg
      841 acaccaaact ccggatccgt ccaccttgcc aacgcctccg cacccacaat gcgccccgtg
      901 gccatgtcca cctgaggctg gtagtgcaca gccatatgcc ccaagcccag cgcctggcgc
      961 atggcatgct ccagcttcat gcgcgacaac agattggcat tcatctgcgg ccgatagaag
     1021 ccaaaattgc cgcgcccacg ctccttgacc tgatacatgg ccgtatcggc ctgcttgatc
     1081 aggtcgtcca gcgactcgcc atcgcgcggg aacatcgaca cgccgatgct gcactggatg
     1141 gaaaacccca tgccatcgag catgaacggg cgctgcaatt catccagcat gcgacgcgcc
     1201 acgccctcgg caatgccgac atcggcgcca tgcaggtaga tgacgaactc atcgcctccc
     1261 aggcggcaca gcatgtctgc ctggcgcagg caaccctgca ggcgttcggc caccagtttc
     1321 agcacgcggt cgccaaaggg atgccccagt gaatcgttga cgatcttgaa acgatccaga
     1381 tccagaaaca ggatggcaaa gctgtccccg gtggcacgcg acagctcaat ggccgcagtc
     1441 accctttcac tcagcaacag gccgttgggc aacccggtca acgcatcacg gtaggccaac
     1501 tgctcaatgc gccgcttggc cgcatgctgc tgcgtcagat cgcgcatgaa gcctatgctt
     1561 tgcgcgtcac ctccctcagc gtcgcgcagc acaacccagg acaaatgcac actgcagtca
     1621 ccgccgtcgc gctggcgcag atgcagctcg ccctcccaga atccgttctg cagccacccc
     1681 tgttgtacct cggacaggaa ggcgctccgg cccttcccct tggcgaacag cgtggtgacc
     1741 gattgaccaa tcactccccc gtcggcggat atcctgagaa gttgctcgca agccggattg
     1801 atgcgcagga tggcgttgcg ccgatcggct atgaagacgg catccagact cgattcaaac
     1861 acccgggccg ccaggcgcag gtcggcctga ttctggatgt cactggtgat atcgcgaaac
     1921 gtatacaccc gccctgctgg ccgcccctga acgagttgag gcacggagcg ctgctccacc
     1981 gtcatgccat tgcgcagaag aaggatgtcg tggctctccc gcatcggttc acgcacaatt
     2041 tcagcacgcc cggcgcgata gacctcactg tcgagcacca ggctttcaat atgcgtccat
     2101 atcgccgcat catcgcgctg caacagcatt tcgtgtggca actgccatat ctcgaccagg
     2161 cgatggttga aggcccgcat ctgcccgttc agattgcagg tcagcactcc atccacagct
     2221 gaatcaaggg ttgcgcgcag ctccgacagc aaggcctcca gctcaagccg ggaggcctca
     2281 tgctccgatc gatcctgcat ggtcaacagc aggcactgtg cagcacctgc attggcgcct
     2341 gcatgaatcg ccagcacgcg acgatccacc ggcaccagcg cctggctgcg catgttgcgc
     2401 acccaggtgt gtgaatggat cccggcagca atgtcggtgg tgggctggcc ccagaaaatc
     2461 tgatcctctg gcgtcgccgc aagctcaagg atgggcatgc cctccatatc tgccacgcgc
     2521 cgcccgacca gcagcgacgc ggcctcattg gcctgcagga tctgcaaaga cttggcatca
     2581 accagccagg cagccagggc caggccatcc caaatggctt gaaagggctg ccggcccata
     2641 ttcatgccgc atccccttcg ccaagtgaat ccagggcgcg cgcaaaaaag tagcaaatac
     2701 gcggttgtgg caacagatat tcaagggcag cacgtggcaa ctgcgccggc ttgaggctgc
     2761 gcagaatgcc aagctcgggc gtttgcgcca ggttcagcaa ggccacctgg ctgcgcggca
     2821 cgtccggttg atgcggcacc acccaagggc gcaggggacg cgccggatcc acaaccatca
     2881 ccaccgccaa acgcccgtcc accagctgca ccaatgagcc cggcggatac acacccatca
     2941 gccgtatgaa ggcacccagc accgacgcat cgaaacttcc ccgcagctgg gcaaaaagct
     3001 ttgccactgc ctcatgcggc gtcaaagcct gatcaccgtg cagcggattg cacagccggt
     3061 cataacgatt gaccaaagcc agaatctgcc cccagcgact gatgtcgtcc gccacaagat
     3121 gcagcgggta gccactgcca tcggccattt cgtgatgctg cgcaattgca atgagcacat
     3181 cgcttggcaa agccatgttc tgcgccagct ccaccgacag tcctacatga ctgtggtagc
     3241 gctgcatgtc ggcagccctc agggcagcac ccggctcacc aatatgggct ggcagattca
     3301 ccttgcccag atcatgcagc aatgcagaca aagccaaacc atgcaactgt tgcgcctgca
     3361 agccgatggc ctgcccaagc agcaaggcca gcaccatcac gttgaccgca tgcgctgccg
     3421 agcgctggct gacaccatcg gccagcaaat gcacggcgca gggacctcgc gtcaggagat
     3481 ctgaaacaca tgttcggatc agcgcttctg cctgctcgcg cgcctgcgcc ggttcagcgc
     3541 gcacatgggc tgagactgcg gcataggtgc gagtggcctc ttgaaaccga agattgcagt
     3601 gctgctggcg cgcattgaat tgcgccaaca gatcggcagc acccagctcc gcttggacca
     3661 tggcatcgct ctcaggctca tccctcgcct cttcagaccg cagcccggcg tttgcggcgg
     3721 ttaccgtgct tttggcaggg atgtaacgca cggattgaag cccgatctca cgcaacacgc
     3781 ggatctggtc gggagatgag agcctgaaac tactcatcgg aaagggatga ttgatccaac
     3841 ccaactcgag ctggatgaac atcccaactc gcagcgcgtc gatatcaatc aggacagcct
     3901 ggttcacgtc aattcagttg taatgggcac gacaaacaca tgtcgacacg gcccatgtgc
     3961 ctgaaacatt atcacctgtt acgcgccaag cactttgttg cacattgtct tttcagggca
     4021 aacaccaggg agcgggttgc ccatgcacaa catccatcca caatgccgtg ccgcccatgg
     4081 cgaagagcac aacgccagcc agccttgagc cccaggaact ggcgacgcga tttacgcggg
     4141 cacggaaact gcgccacaga attggccccg ccaccaacca aaccccactg cccacaccaa
     4201 agagcaccat gcacagcgca ccgcgcagca cactgccgct cagggccgca gtcagcagag
     4261 cggtatagag caaaccacaa ggcaacagcg cccaggccat gcccgtgacg aacagtcccc
     4321 ctgggcgtgc caccagagca cgcaccttcg tccagaccga acgaccggca tgctccagcc
     4381 acgctggctg acgcgcctgc agcatcatca gcagacccca ggccagaatc agcacatgca
     4441 gcaatgtcca ggccggacgc aaggcagcgg tgttttggcc aagccatgca aggcggtcca
     4501 tcgcccaggc agccagggct ccggcgaacc catagcccat cagccgcccc agatgaaaac
     4561 ccgccgtgcg ctgcagagtt ccagcctgcg gcgccagccg caccacccgg gtatcacccg
     4621 ccggcgccgc gcccttcccc gtcaaagccg cacagggtgc ggcacacatg acgagacaat
     4681 gcgtcccgcc catcaaaccc atgaaaaaag ccgtcgatgc cagagcccat aacatggctc
     4741 aaatgatgcg ggaaaagcgc gcgcggttcc tatctgcctg taaatacctg tcaaatacca
     4801 tggccacacc gcgcacaaag aaccacccca tggttgtgac cttgacgccc tcgggactga
     4861 tgctgaccaa cccttgctcg gccatttctt caagctgccg cagctccgct tcaaagtaac
     4921 tgcggaagtc gatgagccag gactgctcca agggctcgaa cagcaactcg ccctgacaca
     4981 tcagagccat gatgacggcg cggcgcacca gatcatcctt ggtcaatgcc agcccccgca
     5041 cgaccggcag gtggccatgg tcgatggcgt cgtagtattc gtccagggtc ttcacattct
     5101 ggctgtacgt tgcaccgacg cggccaatgg cggatacgcc caggccaatc agatcacagt
     5161 caggctgcgt gctatagccc tggaaattac ggtgcaaccg cccctggcgc ttggccaccg
     5221 ccagcgcatc ctcgggcagt gcaaagtgat ccatgcccac gtagacatag ccggcgttca
     5281 tgaatgcatc aatggagcga gccagcatcg acaccttggc cgacgccatg ggcagttctg
     5341 cggtgatgat gcggcgttgc gacttgaaac gctcgggcaa atgggcgtag gcatagagcg
     5401 ctatccgatc aggtcgcagt tgcgcaacct gctccagggt acggtcaaat gactctggcg
     5461 tctgccttgg cagaccatag atcagatcca cgttcaccga gtcgaaaccc agcttgcgcg
     5521 cgctttcaac cagaccaaac accttttctg ctggctgtat gcggtgtacg gctttctgta
     5581 cctctgggtc gaagtcctgc accccgaagc tgagccgatt gaagcccagc tcagccagca
     5641 acgccagccg ctgttcatca accgtgcgcg gatcgacctc tatggagtac tcccctcctg
     5701 gcaccaacac aaaactgcgc ttgagcatgc ccatgagctc acgcaagccg tcatcggaca
     5761 aaaaagtcgg cgtcccacca ccaaagtgca gctgactgac atgctggccg acaccgcagt
     5821 gcgcggtgtg caggtcaatt tcccggctca ggtaacgcag atatacatcg gcccgctcgg
     5881 ggtggcgggt aatgatcttg ttgcaggcac agtagtagca caacgattcg caaaacggta
     5941 tgtgaatgta cagcgacaac ggcatggcct tggccactga tcccacgcgg cgctgttgca
     6001 atgccaggat gtactccttt tcaccaaaag cctccacgaa acgatcggcg gtagggtagg
     6061 aggtatagcg cggccccgga acgtcaaagc gacgcaagag ctcggtggta acgacagtca
     6121 tgaaagggtt ccttgcaatt cgtgcgcact gtgccgtaga gtccaccctg tgtccttgac
     6181 gccgatcaag cccccatgaa gccgagttga cgacaatttg accttggatc aaagtcactc
     6241 aacgaatcgc gacatcccaa gtctcggtta cattcggctg taagtccgta ccatgaaccg
     6301 agtatccatg tcttccaacc tgcaaaccat caaagtagcc tgctccaatt gcaacctgcg
     6361 cgagctgtgc atgcctgtgg gtctggatga ccagcagctc aagcgcattg atgacatcgt
     6421 tgcagttcgc cgcaaggtca agcgcggcgg aaccttgttt cgcaacgggg aggcatttac
     6481 ctcgctcttt gccatccgca caggattctt caaaacctgc gtagcaaccg aagatgggcg
     6541 cgaccaggtc accggattcc agatggctgg agaaatcatc ggtctggatg gcatcgtcaa
     6601 cgaccgccac acctgcgatg ccgtggctct ggaagacgcc gaggtttgcg tcatgccctt
     6661 tgagcgtatc gaggaactgt cgcgcgtagt tacggcactg cagacccatg tgcacaagat
     6721 catgagccgt gaaatcgtcc gtgagcatgg ggtgatgttg ttgctgggca gcatgcgcgc
     6781 cgaggaacgc ctggccgcct tcttgctgaa cctggtgcag cgcctgcatg cgcgcggctt
     6841 ttcgcagtca gaactggtgc tgcgcatgac ccgcgaagaa attggcagct acctgggcct
     6901 gaaacttgaa accgtgagcc gcaccttctc caaattcgtt gaggagggca ttgtggaagt
     6961 caagcagcgc catgttcgca ttgtgaatac ggaagccttg aagcgaattg tgaacagcca
     7021 gcaggcttgt cactaaactt gggcgattca ggcatcccgc attgtcagga tcatgacacg
     7081 tacttggggt tatcagctgg cctgtccgtc tccgacgact cccgatggtt gaactcgaag
     7141 ggagagaacg acaacagggt cgtcaaagcg cctgatatcg taatcaccag ccagaacaag
     7201 aaaaacgcga cggtatagac gccttcgcgc gacagattca caggctggtc aaaccactgc
     7261 aacgcctccg gatcgacaaa ggcaaaaacc accatctcca tgacaccggc catgagaaac
     7321 gcaggccacg caatccacat cagtcgttgc atccacatcg aagcacctcc aagctgttgt
     7381 gctcgcagac tagcactaca tgcacggcaa ctgtctcctt gattcccctt aggccccaga
     7441 atcagcgtgg ttgatcctgc accggggtag cagcgtggtt gcgccccttg gttgctggcg
     7501 tcatgctcat ctgagagttt tgcaatgtct tgttgatgtc aagaccccgt tgatagtagt
     7561 cttccgtcaa cactggatct ggccgtgcta acgccagcca aagtgtgatg aagccagcga
     7621 ttaccacgat caccggcccg gcaatgatca accagacatg gccatacttc caccaaggct
     7681 gagactcagg cgtcgaattc gctgatgagt tgggtgacat gactgcggac tccctgggcc
     7741 ttccgaggcc caatacaaaa aaatcaatac aaaaacagcg gatgctcgta catcaaccct
     7801 tagcgcggca ccaggaacac ggatttttct gccacctggc ctttgtcatc ctgggcacgg
     7861 acatcaaaat acacggtgtg tgagccaggc gctgccgatc catagggaat ttgcagccgc
     7921 acgaccaccc agcgagattc tgccgggccg atgtcaatct ccggttcgga gatcacctca
     7981 agcccgtcca gtccacgtgc actgatcaca tagcgctgcg ccccctcggt ggcgttcatg
     8041 atttgcagtc ggtaaatgtt ttcaagcttc ccgcctgcca cgatgcgcga cagagccgct
     8101 cgatccctca cgatatctac cttcaatggg gtacgcacca ccaagctggc catcatccct
     8161 atgcacaagg cgatcaaaat accggaataa aacaataccc gtggccgcag gacacgtttg
     8221 agcatctgcg actgcgtcca gtggttcttc acgccgttct gcgtggaaaa ccgaatcagg
     8281 ccgcgtggat actgcatctt gtccatcacg gtattgcagg catccacgca caggccgcag
     8341 ccaatacagt cgtactgcag cccatcgcgg atatcgattc ccgtggggca gacctgtacg
     8401 cacagtgaac aatcgatgca atcacccaga cccttggcct tgtagtccac cgccttggtg
     8461 cgcgggccac ggggctcgcc acgctccggg tcatatgtga caaccagggt gtcgttatca
     8521 aacatcgcgc tttggaagcg cgcgtacgga cacatgtatt tgcatacctg ctcacgcaag
     8581 tagccggcgt tgccgtaagt cgccaagccg tagaaaaaca cccagaaaat ctgccagctt
     8641 ccgtgcagcg acataagttc gccacccaac tcccggatgg gtacaaaata tccgacaaat
     8701 gtaaaaccgg tccagaatga cacggtaatc cacaccagtt gcttcagcga ttttttccag
     8761 gtcttttcaa atgtccaggg gccattatcg agccggatgc gcgcactgcg atcgccttcg
     8821 atcttgtgct ctatccacat aaagatctcg gtataaaccg tctgtggaca ggaaaatccg
     8881 caccacagcc gccctgccac cgccgtgaac aagaaaagtg caagcgccga gataatgagc
     8941 agacccgtca gatagatgaa atcctgcggg tagagcacca gaccgaagat gtagaagcgc
     9001 cgagcaccca gatcaaacaa taccatctgg cgctcacccc actgcagcca gggcagacca
     9061 taaaagatca actgggtgac atacaccatg atccagcgcc aattggcgaa tacgccactg
     9121 atcgagcgcg gatagacctt tttctgcgcc tcgtacaaag atgcggattc ggtcttcggc
     9181 ttttctgccg gcgctgccgc aatggggata accttgcggg gctttccgtc ggctggcttc
     9241 atgttgaaaa tctcttggtg caagacgggc ttgcaaaaca aaaaaagccg gcacccctag
     9301 gaggggctgc cggccgatac aaatgcctta ttttgcagcg ccgggcttgt tggacatgcc
     9361 ccagacatag gcggtcagca cattgatctg ctgatctgtc agtttgccag cctgggccgg
     9421 catttgattg gtcttgccat cgttgatcat agccacgatc gctgcctcgc cccagccatg
     9481 cagccagatg tcatcggtca gattaggggc tcccatggca gtcatgccct tgccgtccat
     9541 gccatggcaa gccgcacagg cggtgaactt ggccttgccc agcgacgcct tgagagaatc
     9601 gtgcggactt cccgacagac ttagcacgta ttgggccagg ttgcgcacgt cgtcgggcga
     9661 acccaccgct gcggccatgg ggggcatcac accgactcga ccatcatgga tggtcttgcg
     9721 aatgtcatcg ggcgtaccac cgtgcaacca atccttgtcc gacagattcg ggaagctctt
     9781 gctaccgcgt gcgtccgagc catgacactg ggcacagttg ttcatgaaca aacgctcgcc
     9841 gatcgccatg gccgcctggt cggctgcaat gtcctcggtt ttcatgctgt cgaaccgtgc
     9901 atacagtggc tgcaactcgg cattggcctt gtcaacttct gcctggtact caccgacttg
     9961 cgtccacccc agctggccct tgaaggtgcc caggcccggg taggccgcca gataggcaaa
    10021 ggcaaagacg atggtgatga caaacagcca catccaccaa cggggcatag ggttgttcat
    10081 ctccctcaga tccacatccc agacatggcc cgtggtgttg tcagcagcgg attcgatctt
    10141 cttgcgtgat gtcagccaca gcagcaaggc acaggcgatg atgccggcaa gggtgatccc
    10201 ggccacataa atagaccaga aattactggt gaagtcgctc atgggttctc tcttcgtatc
    10261 ggttggagca atcaatcgtc attggcgaag ggcagctgcg ctgcctcatc gaactgggcc
    10321 cggttgcgag ccgtataggc ccagtaccaa atacccagaa agcacgcgag cgaagccagc
    10381 gtgaccacga tgcgcatggt ggtgatgtcc atggcaagct ctctttactt gagcgcggtg
    10441 cccagcacct gcaggtaggc aatcacggct tccatctcgg tcttgccctt gacggcctcg
    10501 gccgagccgg cgatttcctc atccgtgtag ggcacgccaa cgcggcgcaa tgcgcgcata
    10561 tgcggagcca tgctggcggc atccacctgg ttcttttcga gccaggggta ggcaggcatg
    10621 ttggactcgg gcaccacatc acgcgggttg accagatgaa tgcggtgcca ttcatcactg
    10681 tacttgccgc caacacgatg caaatccggg ccggtgcgct tggaacccca ctggaacggg
    10741 tagtcgtaga caaactcacc ggcaaccgaa tagtgaccat agcgcagcgt ctcggcgcgg
    10801 aacggacgaa tcatctgcga atggcagttg tagcagccct cgcgttgata gatgtcgcgc
    10861 ccggccagct gcagcgccgt gtagggcttg aggccctgaa ccggttcggt ggtggacttc
    10921 tggaagaaca gcggaacgat ttccaccaaa cctcccatgg cgatcaccac caggatcagg
    10981 acgatcatga ggaaattgct ggtttccacc ttctcatggg tgaaaccgga gcttgtttgc
    11041 ttgtttgtat tagacatcta cggattcctc agacctcaag cgtgcgccgg attgacggcc
    11101 ggaatggcca ccttcacgga acgaccggaa attgcggtca tccaggtgtt ccaggccatg
    11161 aagcacatac cagccagata caggacaccg cccaggaaac gaatggcata gaagggatag
    11221 gtggctttca cgctttcgac aaaggtgtag gtcagcgtgc catcagggtt gaccgcgcgc
    11281 cacatcaggc cctgcatcac gccggcaatc cacatcgcgg cgatgtacag cacgatgccg
    11341 atggtggcca gccagaagtg gatttcaatg gccttgatgg aatgcatctt ttcgcgaccg
    11401 aacaggcgcg ggatcatgta gtagagcgaa cccatggtca ccagccccac ccagcccagc
    11461 gcgccggagt gcacgtgacc cacggtccag tcggtgtaat gcgacagagc gttgacggtc
    11521 ttgatggaca tcatcgggcc ctcgaacgtg gacatgccgt agaacgacag cgacacgatc
    11581 aggaagcgca ggatggggtc gtcacgcagt ttgtgccagg cgcccgacaa ggtcatgatg
    11641 ccgttgatca tgccgcccca gctgggagcg agcaggatga gcgagaagac catgccgacg
    11701 gactgcgtcc agtcaggcag ggccgtgtag tgcagatggt gcgggccggc ccacatgtag
    11761 gtgaagatca gggcccagaa gtggacgatg gacaggcggt agctgtacac cgggcggccg
    11821 gcctgcttgg ggatgtagta atacatcatg cccaggaagc ccgtggtcag gaaaaagccc
    11881 acggcattgt ggccatacca ccactgcacc atggcatcct gcacgcccgc
//
LOCUS       DAOMTQ010000096        11873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_992_length_11873_cov_25.932730, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000096 DAOMTQ010000000
VERSION     DAOMTQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_992_length_11873_cov_25.932730"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PK518_10140"
     CDS             complement(<1..750)
                     /locus_tag="PK518_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRM49316.1"
                     /translation="MQQIVRQLAAEIKITEAQVRAAVDLLDGGATVPFIARYRKEATG
                     GLDDVQLRELDARLAYLRELDDRRAAVLKAIDEQGKLTDVLRASIAAAATKQELEDIY
                     LPFKQKRRTKGQIAREFGIEPLADRLLADPTLDPHAEAQAFCQPATLLDDGKPGPDFS
                     TTFAVLDGVRDILSERWAEDAALVQGLREWLWDEGLLRSKLVDGKNANDPEVAKFRDY
                     FDYDEPIGRVPSHRALAVFRGRALEILEAKLV"
     gene            851..1252
                     /locus_tag="PK518_10145"
     CDS             851..1252
                     /locus_tag="PK518_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49317.1"
                     /translation="MNAPPPPIRTECPPGSCVCQRDALMQASGGDTRILRLTREEEKR
                     LVQRLEQITSLADLRHMERRLFEQLGVRLTIGTSPNGVRSLRGITVLVHEQPGLCRKT
                     RQAIPAAIKKSMETRSGIVFELLDEGGLFGG"
     gene            complement(1350..2768)
                     /locus_tag="PK518_10150"
     CDS             complement(1350..2768)
                     /locus_tag="PK518_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49318.1"
                     /translation="MSARPPAAAITAVSDGLPQTERRQAMRVIILGLTLAVLDTSLVN
                     LALPDIARLLQSDAAHSVWVVNAYQLATLIVLLPLSALGERWGYRRVYLVGMAVFAVA
                     SLGAMLAQSMAALIAARALQGLGAAGVMAVNAALVRLIYPRAQLGQGMALNSLVVATA
                     SMAGPTVAAAILSVASWHWLFAMNLPLGLYIWRLGRRALPANPPSAHDAPRFSAIDLL
                     LNGAMFTLIFLGGSRLGVGAAAQTGGGAAGAWLLLAGLAVGALFLWRQRGLAAPLFPV
                     DLLRIPVFALSMGSSVGAFCAQMMGFLALPFLLLQAHGRSHLQAGLLITAWPLATALV
                     APLAGRLIGRYPDGLLGGIGMAVFAAGLLSLGLMPAQPADWDMAWRMALCGAGFALFQ
                     SPNNHTIVTSAPLHRSGAASGMLGTARLTGQTLGAVLLAAIFALRPGHDGSAESLALL
                     LAAGWAVAAGVCSSLRVRQGPV"
     gene            2972..3595
                     /locus_tag="PK518_10155"
     CDS             2972..3595
                     /locus_tag="PK518_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRM49319.1"
                     /translation="MPLPSRTLLLHAALTLSAMALVLPAGAATPEDAGADDMPATPMQ
                     AVQPPLILSPDGSLVIDTRARLAWPRCVEGMRWSGNGCTGVPQLLSYGQAQALARQRW
                     QAEGVRWRLPRVNELRRLVNRKALPPSVDTQLFPAMPRDWLWTGTASVNTNTVNPYAY
                     GNVARGGAGESSMTVQQSWAVDMASGEARGDVGRATRLHVQLVRPAP"
     gene            complement(3673..4596)
                     /locus_tag="PK518_10160"
     CDS             complement(3673..4596)
                     /locus_tag="PK518_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRM49320.1"
                     /translation="MTELILHHYPASPFAHKARCVLGFKQLAWRSVTVPSVMPKPDVV
                     ALTGGYRRTPVLQIGADIYCDTALICDVLEHLRPEPTLYPPHLKGVSRVFAQWADGTL
                     FAAAMAYNLQPRAAEALFADYPAGAAQAFSADRRAMGMAHLAVPDAAAAYRSYLRRIA
                     NMVEEHDFLFGAEPCVADFAAYHPLWFTRHCVPMMADILSATPAVLEWMDRLAALGQG
                     RAEKFSAQDAITVAAGALPQPMPPQVFQDEHGIALGSRVSIAAESFGTEPTEGLLVAA
                     TRMHYTLARSDARAGTLHVHFPRIGYVLRAI"
     gene            complement(4659..5675)
                     /locus_tag="PK518_10165"
     CDS             complement(4659..5675)
                     /locus_tag="PK518_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRM49321.1"
                     /translation="MPRNQQILLDNRPQGEATAGNFRLVASDTPALQDGQVLVRHHYL
                     SLDPYMRGRMNEGKSYAACQSLGQVMIGGTVGEVAESRHPAWAVGDAVVGMGGWQEYS
                     VVDGNAPGLLRKVDTSHVPLSYYLGAVGMPGVTAWYGLVQIIDPKPGETLVVSAASGA
                     VGSAFGALAKARGCRVVGIAGGPDKCRYVTDELGFDACIDHRAHGDLKSMAAALKEAC
                     PDGIDGHFENVGGHILDAVLLRANAFARVALCGMIAGYDGQPLPLHNPALILINRMKI
                     EGFIVSEQMQVWPQALAELGALVASGRLRPRESIAQGLAAAPEAFLGLLKGKNFGKQL
                     VKLI"
     gene            complement(5752..6180)
                     /locus_tag="PK518_10170"
     CDS             complement(5752..6180)
                     /locus_tag="PK518_10170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRM49322.1"
                     /translation="MLDFGADIPFVKELGFSLQRMHGGESELHYEARAQHTNSYGVMH
                     GGAVMTLLDVAMATAARSDTPDMGVVTIEMKSSFIRPARGPMVAKGRMLERTRSMAFA
                     EAWVYDAEGRLCSHSTGTFKYVPKAQPAAGQGAGTISTDG"
     gene            complement(6252..7043)
                     /locus_tag="PK518_10175"
     CDS             complement(6252..7043)
                     /locus_tag="PK518_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM49323.1"
                     /translation="MARTVQQLFDLSGKTALVTGGSRGLGLQMAQALGEAGARIMLSS
                     RKADDLEQATAQLQAAGINARWIAADCAREDDIQRLASETLQRMGDVDILVNNAGATW
                     GAPAEDHPLAAWDKVMNLNVRGYFLLSQAIARHSMIPRRGGRIINVASIAGLAGNPFA
                     MKTIAYNTSKGAVLNFTRALAGEWGAHGINVNAICPGFFKTRMTTVLIETLNEERMTA
                     QAPLRRLGDDEDLKGITLLYASDAGKHITGQWLAVDGGVSAILGG"
     gene            7224..8483
                     /locus_tag="PK518_10180"
     CDS             7224..8483
                     /locus_tag="PK518_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49324.1"
                     /translation="MPSADPQRWYFGWNIVAAATVLTALTVGMRLGLGPLFLPMAEDL
                     GFSRSLLSTIVAVGMLCYGAAMPLAGWLVARRGTRYVLLCGTAMLVLATLWAVNTRTP
                     LGLMLSFGVLMSLGAGFTSPIALTPIISRWFNRRRGMALFFLSTGSMAGIAIMTPALG
                     LALQHLSWQATLLGFAVLFSAITVPSALLVMRDQAPPDGDAPLPGSAAHAANPVAPVG
                     QHYTVLQAMRTATFLKITLGLFACGFSMNLLGTHGMPMLMDHGFDATTSALGIGLIGV
                     VAIPSTVVLGRLADRMPRRKLLAAIYCVRGFGFFSLLLAGSTLELYGTSVIGGIAWAG
                     SIALSSAILADIYGVRLVGVLYGWAYLGHQVGAAISSWLGGWGFEHLGTHWLAFGLAG
                     ALLMLASGVALLLPGKAAPAPARASTA"
     assembly_gap    8846..8945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8946..9930
                     /locus_tag="PK518_10185"
     CDS             <8946..9930
                     /locus_tag="PK518_10185"
                     /inference="COORDINATES: protein motif:HMM:NF021926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HRM49325.1"
                     /translation="LAGGGSIGLLLVQDPDGDYGTAAIRLQGNAADCAERALQAALHD
                     AGCPGELPELVWIYQAPGQEEAVIEGLRRIVSDRCPIIGGSSADDDIGGRWRQLGPDG
                     PLCDGLVVGVLFPSGGISHVFQGGYEPSGHSGTITRVGATAAAGGAARHIIAIDEQPA
                     AEVYNRWLGGALEPQLAAGGNILRATTMFPLGVDAGRIGEVRCHRLIHPERILPGGAL
                     STFAQVEEGMRLYAMRGHKSHLVERAGLVAAAAAASLPGGANTLAGALVVYCAGCMFA
                     VGEDMPQVAQAVAQRLAGAPFVGCFTFGEQGAMQGRNMHGNLMISAIAFGK"
     gene            9941..11872
                     /locus_tag="PK518_10190"
     CDS             9941..11872
                     /locus_tag="PK518_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM49326.1"
                     /translation="MEDTEKLRETIICMRREIDSLRTENLHSQLLLSALDAVLCTSSE
                     GDLFSGVFAAVKPVFECSLALVLLELHPGSDAVQCVATSRPELMHHSWPVGPFLHKVL
                     AGRTVSGVADPLLDAWPQGPSGTLSAQQPALYLPLLIQGRRSLMVLVREPHQSGFDRS
                     HVALARKFSLLGTHVMAAQRATLKEAESRRLRELQDKLQTSRDALRFRANHDQLTGLP
                     NRAHVQELVTALIARKPPGGKLALAFINLDDFKRVNDLHGHAAGDALLREVTARVQSQ
                     IRQADVFGRINGDEFVIALDPVGQSSDVAQVIERIRARLQQPLTLHGTQIQPSASIGV
                     AMFPAHGGDYETLRRNADLAMNRAKAAGKGGLDFFTPDLGREAQERLSLEQRLRDAVS
                     AGEFCCALQRKVDIRSGAITGFEALARWVDAEGVMRMPGQFLPLASRLQLLDDIALQV
                     VDDLTRNLPQLDAMFGTAIKYSINVSPAQAIDTDFMRRLTQRLPSGQAQRFIVELTEE
                     SLADTGLLEAHVLPMLRQAGVHVSIDDFGTGYSSLSKLASLTVDELKIDRSLVQTIQQ
                     LPRNQLILRAIESLGTALGLSIVAEGIETEQERDFLLANTAITMGQGFLFHKPQLIAT
                     LVGETPSMPSPAPDRLESA"
BASE COUNT         1898 a         4092 c         3909 g         1874 t
ORIGIN      
        1 caccagcttg gcctccagaa tctccagcgc ccggccgcgg aacaccgcca gcgcccggtg
       61 gctgggcacg cggcctatcg gctcgtcgta gtcaaagtag tcgcggaact tggcgacctc
      121 ggggtcgttc gcgttcttgc catcgaccag cttggagcgc agcaggccct cgtcccacag
      181 ccactcgcgc aggccttgca ccagtgccgc atcctcggcc cagcgctcgg acaggatgtc
      241 gcgcacgccg tcgagcacgg caaacgtcgt cgaaaagtct ggccccggct tgccgtcgtc
      301 aagcagggtg gcgggctggc agaaggcctg ggcctcggcg tgcgggtcca gcgtagggtc
      361 ggccagcagt ctgtcggcca gcggctctat gccgaactcc ctggcgatct ggcccttggt
      421 acggcgcttt tgcttgaagg gcaggtaaat gtcttccagc tcctgcttgg tggcggcggc
      481 cgctatggac gcgcgcagaa catcggtcag cttgccctgc tcgtcaatcg ccttgagcac
      541 cgcggcgcgc cgatcgtcca gctcgcgcag gtaggccagg cgcgcgtcca gctcgcgcag
      601 ctgcacgtcg tccagcccgc ccgtggcctc cttgcggtag cgcgcgatga agggcacggt
      661 ggcgccgcca tcgagcagat ccacggcggc gcgcacctgg gcttcggtga ttttgatttc
      721 tgcggccaac tgccgaacga tttgctgcat gtacctggaa acgagtcgct aaaacgaagc
      781 gcgggagttt gccacagggg ccacgggcac tgtgacaggc tgtaggtgcc cgccataatc
      841 aagggccgcc atgaacgcac cgccccctcc cattcgcacc gaatgcccac ccggcagctg
      901 cgtgtgccag cgcgatgcat tgatgcaggc cagtggcggc gacacgcgca tcctgcgcct
      961 cacgcgcgag gaggaaaagc gcctggtgca gcgcctggag cagatcacca gcctggccga
     1021 cctgcgccac atggagcggc gcctgttcga gcaacttggc gtacgcctga cgataggcac
     1081 cagccccaac ggggtgcgca gcctgcgcgg catcaccgtc ctggtgcacg agcagccggg
     1141 cctgtgccgc aagacgcgcc aggccatccc cgcggccatc aagaaaagca tggagacgcg
     1201 gtcggggatt gtttttgaac tgctggatga gggtgggctg tttggcgggt agccgggtgc
     1261 gcgccttggt caatcacatc aaaaacaaga gctgctcacg ccctaccaga gagcctgaac
     1321 ggcctaaatt aattggaatc tgaccccaat taaactggcc cctgccgcac gcgcagcgag
     1381 ctgcacacgc ctgcggccac cgcccacccc gccgcaagca gcagcgccag cgactcggcg
     1441 ctgccgtcat gccccgggcg cagcgcgaag atggccgcca gcagcacggc gcccagcgtc
     1501 tggccggtca ggcgcgctgt gcccagcatg ccgctggccg cgccgcttcg gtgcagcggg
     1561 gccgaggtga cgatggtgtg gttgttgggc gactggaaca gcgcaaagcc cgcgccgcac
     1621 agcgccatgc gccaggccat gtcccagtcg gccggctggg cgggcatcag gcccagcgac
     1681 agcaggccgg cggcgaacac cgccatgcct atgccgccca gcaagccgtc cggatagcgg
     1741 ccgatcaggc gcccggccag cggcgccacc agggccgtgg ccaggggcca ggcggtgatg
     1801 agcaggccgg cttgcagatg gctgcgacca tgggcctgca gcagcaggaa aggcagggcc
     1861 agaaagccca tcatctgcgc acaaaaagcc cccaccgacg agcccatgga cagggcgaac
     1921 accggaatgc gcagcaggtc caccggaaac aacggcgccg ccaggccacg ctggcgccac
     1981 agaaacaagg cccccacggc caggccggcg agcagcagcc aggcgcctgc cgcgccgccc
     2041 ccagtctgcg cggcagcgcc cacgcccagc ctcgatcccc ccaggaagat cagcgtgaac
     2101 atggcgccgt tgagcagcag gtcgatggcc gaaaagcgcg gtgcatcgtg cgccgatggt
     2161 gggttggccg gcaatgcgcg ccgccccagg cgccatatgt agaggcccag cggcaggttc
     2221 atggcaaaca gccagtgcca ggaggccacc gacaaaatgg ccgccgccac cgtgggcccg
     2281 gccatcgagg ccgtggccac gaccagcgaa ttgagggcca tgccctggcc cagctgggcg
     2341 cgcgggtaga tcaggcgcac cagggcggcg ttgacggcca tcacgccggc cgcgcccagg
     2401 ccctgcaggg cacgcgcggc aatcagcgcc gccatggact gcgccagcat ggcgcccagc
     2461 gaggccacgg caaacaccgc catgcccacc aggtacacgc gccggtaacc ccagcgctcg
     2521 cccagcgcgg acagcggcag cagcacgatc agcgtggcca gctggtaggc gttgaccacc
     2581 caaaccgaat gcgccgcatc ggactgcagc aggcgcgcaa tgtcgggcag ggccaggttc
     2641 accaggctgg tgtcgagcac ggccagcgtc aggcccagga tgatgacccg catcgcctgg
     2701 cggcgctcgg tttgcggcag gccgtcgctg acggccgtga tggccgcggc tggcgggcgt
     2761 gcgctcatgg ccgccccgca agcgaggggc gcagcgccat gcggcgggag gctggtatca
     2821 acatcggccc gattgtcgcc agactcgccc aagcctgccg aacgcgcaca acaaggccct
     2881 gaaaccgccg ctattgcccg cattgccggg ccaaccctgg cgggacaatg cccacagccc
     2941 ccattgccaa gcccacatcg caccatccgc catgcccctg ccctcccgca ccctcctgtt
     3001 gcacgccgcc ctgacgcttt cggccatggc actggtgctg ccggcgggcg ccgccacgcc
     3061 agaagacgcc ggcgctgacg acatgccggc cacacccatg caggcggtgc agccgccgct
     3121 gatcctgtcg cccgacggca gcctggtgat agatacccgc gcgcgcctgg cctggccgcg
     3181 ttgcgtggag ggcatgcgct ggagtggcaa cggctgcacc ggcgtgccac agctgctgag
     3241 ctacggccag gcccaggccc tggcgcgcca gcgctggcag gccgagggcg tgcgctggcg
     3301 cctgccgcgc gtgaacgagc tgcgccgcct ggtcaaccgc aaggcgctgc cgccatcggt
     3361 ggacacgcag ctgttcccgg ccatgccgcg cgactggctg tggacgggca cggccagcgt
     3421 caacaccaac accgtcaacc cctacgccta cggcaacgtg gcgcgcggcg gcgcgggcga
     3481 gagcagcatg acggtgcagc aaagctgggc cgtggacatg gccagcggcg aggcgcgcgg
     3541 cgacgtcggc agggccacgc ggctgcatgt gcagctggtg cggccggcac cctgagtttt
     3601 ttactactta atcaatagct atttgcgctt gatgcgcggg cgcttgcggc cgatttttct
     3661 caaacctctt gatcagatcg cgcgcagcac atagccaatg cgcggaaagt gcacatgcag
     3721 cgtgcccgcg cgcgcgtcgc tgcgcgccag ggtgtagtgc atgcgcgtgg cggcgaccag
     3781 caggccttcg gtaggctcgg tgccaaagct ttcggcggca atgctcacgc ggctgcccag
     3841 ggcgatgcca tgctcgtcct ggaacacctg gggcggcatt ggctggggca aggcaccggc
     3901 ggccaccgtg atggcgtcct gggcgctgaa cttctcggcc cgtccctggc ccagggcggc
     3961 caggcggtcc atccactcca gcacggccgg cgtggcgctc agaatgtcgg ccatcatggg
     4021 cacgcaatgg cgcgtgaacc acagcgggtg ataggcggca aagtcggcca cgcagggctc
     4081 ggcaccaaac aggaagtcat gctcttccac catgttggca atgcgccgca ggtaggagcg
     4141 gtaggccgct gccgcgtccg gcacagccag gtgcgccatg cccatggcgc gccgatccgc
     4201 gctgaaagcc tgggcggcgc cggcggggta gtcggcaaac agcgcctcgg ccgcgcgcgg
     4261 ctgcaagttg taggccatgg cggcggcaaa cagcgtgccg tcggcccatt gcgcgaacac
     4321 gcgcgagacg cccttgaggt gcggcgggta caaggtgggc tcgggccgca gatgctccag
     4381 cacgtcacag atcagcgccg tgtcgcaata aatgtcggcg ccaatttgca ggacgggcgt
     4441 gcgccggtag ccacccgtga gggccaccac gtcgggtttg ggcatcacgc tgggcacggt
     4501 gaccgacctc cacgccagct gcttgaagcc cagcacgcag cgcgccttgt gcgcgaacgg
     4561 cgatgcgggg tagtggtgca gtatgagttc ggtcatggtg cgactcctgc gggcggcggc
     4621 cggggccatc ccggcatggg ggctggccgc ccaagcgctt agatcagctt caccagctgc
     4681 ttgccaaagt tcttgccttt gagcaggccc agaaacgcct cgggcgccgc cgccaggccc
     4741 tgggcaatgc tctcgcgcgg gcgcagccgg ccgctggcga ccagcgcgcc cagctcggcc
     4801 agcgcctggg gccagacctg catctgctcg ctgacgatga agccctctat cttcatgcgg
     4861 ttgatgagga tcagcgccgg gttgtgcagc ggcagcggct ggccgtcgta gccggcgatc
     4921 atgccgcaca gggccacgcg ggcgaaggcg ttggcgcgca gcagcacggc gtccaggata
     4981 tggccgccca cgttctcgaa atggccgtct atgccgtccg ggcaggcctc cttcagcgct
     5041 gcagccatgc tcttcagatc gccatgcgcg cgatggtcta tgcaggcgtc aaagcccagc
     5101 tcgtcggtca cgtagcggca tttgtccggc ccgccggcaa tgcccaccac gcggcagccg
     5161 cgtgccttgg ccagggcgcc aaaggcgctg cccacggcgc cgctggcggc gctgaccacc
     5221 agggtctcgc cgggcttggg gtcgatgatc tgcaccagcc cataccaggc cgtcacgccg
     5281 ggcatgccca ccgcgcccag gtagtacgac aaggggacat ggctggtatc gaccttgcgc
     5341 agcaggccgg gcgcgttgcc atcgaccacg ctgtattcct gccagccacc catgccgacc
     5401 accgcgtcgc cgacggccca ggccgggtgg cggctctcgg ccacctcgcc cacggtgccg
     5461 ccaatcatca cctggcccag gctttggcag gctgcgtagc tcttgccctc attcatgcgc
     5521 ccgcgcatgt aggggtccag gctcaggtag tgatgccgca ccagcacctg gccgtcctgc
     5581 agcgctgggg tgtcactggc gacgaggcgg aagttgccgg ccgtggcctc gccctgcggg
     5641 cggttgtcga gcaggatttg ctgattgcgt ggcatggctg tgcgcctcct ttacagtttt
     5701 gatagctgtt cacgcttatg gggtaagcgt tagcggccga ttttgctcat atcacccatc
     5761 ggtggagatg gtgcctgcgc cctgccccgc cgccggctgg gccttgggca cgtacttgaa
     5821 ggtgccggtg gagtggctgc acaggcgccc ctcggcgtca tacacccagg cctcggcaaa
     5881 ggccatggag cgcgtgcgct cgagcatgcg ccccttggcc accatggggc cgcgcgccgg
     5941 gcgtatgaag ctgctcttca tctcgatggt gaccacgccc atgtccggcg tgtcgctgcg
     6001 cgcggccgtg gccatggcca cgtccagcag ggtcatcacg gcgccgccat gcatcacgcc
     6061 gtaggaattg gtgtgctggg ctcgcgcctc atagtgcagc tccgactcgc cgccatgcat
     6121 gcgctgcagg ctgaagccca gttctttgac gaagggaatg tcggctccaa aatccagcac
     6181 gggcggctcc tagaaaaatt gagacggcaa gaatagcccc aggcggcgct ttgcgcttgg
     6241 ccgctggcca atcacccgcc caggatggcg ctcaccccgc catcgacggc cagccactgg
     6301 cccgtgatgt gcttgccggc atcgctggca tacagcagcg tgatgccctt cagatcctcg
     6361 tcgtcgccca ggcggcgcag gggcgcctgg gccgtcatcc gctcctcgtt caaggtttcg
     6421 atgagcacgg tggtcatcct ggtcttgaag aaacccgggc agatggcgtt cacgttgatg
     6481 ccatgggcgc cccattctcc ggccagcgcg cgcgtgaagt tgagcaccgc acccttggag
     6541 gtgttgtagg caatggtctt catggcaaac gggttgcccg ccaggccggc aatggacgcc
     6601 acgttgatga tgcgcccgcc gcgccgcggg atcatgctgt gcctggcaat ggcctgcgac
     6661 agcaggaagt agccgcgcac gttcaggttc atgaccttgt cccaggcggc cagcgggtga
     6721 tcctcggccg gcgcgcccca ggtggcgccg gcgttgttca ccagaatgtc cacgtcgccc
     6781 atgcgctgca gggtctcgct ggccaggcgc tggatgtcgt cctcgcgcgc gcagtcggcg
     6841 gcgatccagc gcgcgttgat gccggcggcc tgcagctggg ccgtggcctg ctccaggtcg
     6901 tcggccttgc gcgagctgag catgatgcgc gcgcccgcct cgcccagggc ctgcgccatc
     6961 tgcagcccca ggccgcgcga tccgccggtc accagggctg tcttgcccga aagatcaaaa
     7021 agctgttgca ccgtgcgcgc catgagtccg tctccgtgag tgaaagtcag cggctattgt
     7081 ggcggccctg tgactgcctg cctgtcgcca gcgtgatgcg cggcgcgaag gcagccggcg
     7141 ccgatgaaga tgcccctggc gtggcgcagc ggcgccgaat ggcgtacaag tagcggtttg
     7201 cgtcccgcag ctccttcccc cctatgcctt ctgcagaccc ccaacgctgg tacttcggct
     7261 ggaacatcgt ggccgcggcc accgtgctca ccgccctgac ggtgggcatg cgcctgggcc
     7321 tgggcccgct gttcctgccc atggccgagg atctgggttt ctcgcgcagc ctgctctcga
     7381 ccatcgttgc cgtgggcatg ctgtgctatg gcgcggccat gccactggcg ggctggctgg
     7441 tggcgcgccg cggcacacgc tatgtgctgc tgtgcggcac ggccatgctg gtgttggcga
     7501 cgctgtgggc ggtgaacacg cgcacgccgc tgggcctgat gctgagcttt ggcgtgctga
     7561 tgtccttagg cgcgggcttt accagcccca tcgcgctgac ccccatcatc agccgctggt
     7621 tcaaccggcg ccgtggcatg gcactgttct tcctgtccac cggctccatg gccggcatcg
     7681 ccatcatgac gcccgccctg ggcctggccc tgcagcacct gagctggcag gccaccttgc
     7741 tgggtttcgc ggtcctgttc agcgccatca ccgtgccctc cgctctgttg gtgatgcgcg
     7801 accaggctcc acccgacggc gatgcgcccc tgcccggcag tgccgcccat gccgccaatc
     7861 cggtagcgcc cgtgggccag cattacacgg tgctgcaggc catgcgcacg gccacctttc
     7921 tgaagatcac gctgggcctg tttgcctgcg gcttcagcat gaacctgctg ggcacgcatg
     7981 gcatgcccat gctgatggat catggctttg acgctaccac cagtgccctg gggatcggcc
     8041 tgattggcgt ggtggccatt cccagcaccg tggtgctggg ccggctggcc gaccgcatgc
     8101 cccggcgcaa gctgctcgcc gccatctatt gcgtgcgcgg cttcggtttc ttctcgctgc
     8161 tgctggccgg cagcacgctg gagctctacg gcacctcggt cataggcggc attgcctggg
     8221 ccggcagcat cgcgctgtcc tcggccatcc tggccgacat ctatggcgtg cgcctggtag
     8281 gcgtgctcta cggctgggcc tacctggggc accaggtcgg cgcggccatc agctcctggc
     8341 tgggcggctg gggcttcgag catctgggca cgcactggct tgcctttggc ctggcgggcg
     8401 cgctgctcat gctggcctcc ggcgtggccc tgctgctgcc aggcaaggcc gcgccggcac
     8461 cggcacgcgc ttcgaccgcc tgaccaatcc gccccggcgc gccgcgggca gcaacgcgcc
     8521 atggcctgcg cgttgcctcg ccccgccatg cccattacca cgatgtaaaa caattgcaaa
     8581 tttgccacaa tatgttttgt cattctttgt acatctggta actttgcagc acgaccagct
     8641 gaacaccaac caaacgcccg tcaagggcca agaaaaccga catcgccatg gtcgcaatga
     8701 agtcctacac cgccagcggc agctgcacgg cacaggtcct ggcaacgttg gcccagaccc
     8761 tggccgaggc gcagccgttc acggcggact ttctgtgcgt gttctacgac gcgctgcatg
     8821 acgaccaggc gatcacgctt ttcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnncctgg ccggcggcgg ctccataggg ctgctgctgg tgcaagaccc cgacggtgac
     9001 tacggaacgg ccgccatccg tctgcagggc aatgccgccg actgcgcaga gcgcgcactg
     9061 caggcagcac tgcacgacgc cggttgcccc ggcgagctgc ccgaactggt ctggatctac
     9121 caggccccgg ggcaggaaga ggccgtcatc gagggcctgc gccgcatcgt cagcgaccgc
     9181 tgccccatca ttggcggcag ctcggcggac gacgatatcg gcggccgttg gcgtcagctc
     9241 ggcccggatg ggccgctgtg cgacgggctg gtggtcggcg tgctgttccc ctccgggggc
     9301 atcagccacg tcttccaggg tggctatgaa cccagcggtc acagcggcac catcacccgc
     9361 gtcggcgcca ccgccgccgc gggcggggcg gcacgccaca tcatcgccat cgacgaacag
     9421 cctgccgccg aggtctacaa ccgctggctt ggcggagcac tcgagccgca gctcgctgcg
     9481 ggcggcaaca tcctcagggc cacgaccatg tttccgctcg gcgtcgatgc gggcaggatt
     9541 ggcgaggtgc gctgccatcg gctgatacac cctgaacgca tcctgcccgg cggcgcgctg
     9601 tccacctttg cccaggtcga ggaagggatg cgcctgtacg ccatgcgcgg ccacaaatca
     9661 catctggtgg agcgcgccgg cctggttgcc gcagcggccg ccgccagtct gccgggtggc
     9721 gccaacacgc tggcgggcgc cctggtggtg tattgcgcgg gctgcatgtt tgccgtgggc
     9781 gaggacatgc cccaggtagc ccaggcggtg gcgcaacgcc tcgccggcgc gcccttcgtc
     9841 ggctgcttca cttttggtga acagggcgcc atgcaagggc gaaacatgca cggcaacctg
     9901 atgatttccg caatcgcttt tggcaaatag gcacaggcgc atggaagaca ccgagaagct
     9961 acgcgagacc atcatctgca tgcgccgcga gatcgactcc ctgcgcaccg agaacctcca
    10021 ttcgcagctg ctgctctctg ccctggatgc cgtgctgtgc accagcagcg aaggcgacct
    10081 gttttccggt gtctttgcgg ccgtcaagcc ggtgttcgaa tgttcccttg ccctggtgct
    10141 gctggagctg cacccaggca gcgatgcagt gcaatgcgtg gcgaccagcc gtccggagct
    10201 gatgcaccac agctggccgg tgggcccttt tctgcacaaa gtgcttgccg ggcgcacggt
    10261 cagcggcgtt gccgaccccc tccttgacgc ctggccgcag ggcccgagcg gcaccctgtc
    10321 agcgcagcag ccggccttgt atttgccgct gctcatacag ggacggcgca gcctgatggt
    10381 gctggtgcgc gaaccccatc aaagcggctt cgaccgcagc catgtcgccc tggcgcgcaa
    10441 gttttccctg ctcggcacgc atgtgatggc ggcccaaaga gccaccctga aggaggccga
    10501 gagccgccgg cttcgggagt tgcaggacaa gttgcagaca agccgcgacg cactgcgctt
    10561 tcgcgccaac cacgaccagc tcaccggcct gcctaaccgc gcccatgtgc aggagctggt
    10621 caccgcgttg attgcgcgca agccgcctgg cggaaagctg gccctggcct tcatcaacct
    10681 ggatgatttc aagcgcgtca acgacctgca cggccatgcc gccggagacg cactgctgcg
    10741 cgaggtgacc gcacgcgtgc agtcgcagat ccggcaggcg gatgtcttcg ggcgcatcaa
    10801 cggcgacgaa ttcgtcatcg ccctcgaccc cgtggggcag agctcggacg tggcgcaggt
    10861 gatagagcgc atacgcgccc ggctgcagca accgctgacc ctgcatggca cgcagatcca
    10921 accctcggca tcgatcggcg tggccatgtt tccggcccac ggcggggact acgaaacctt
    10981 gcggcgcaac gccgacctgg ccatgaaccg cgccaaggcg gccggcaagg gcggcctgga
    11041 cttcttcacg cccgacctgg gccgggaggc ccaggagcgc ctgtcgctgg agcagcgcct
    11101 gcgcgacgca gtcagcgccg gtgagttttg ctgcgccctg cagcgcaagg tggacatccg
    11161 cagcggtgcc attaccggct tcgaggccct ggcgcgctgg gtggatgcag aaggcgtcat
    11221 gcgcatgccg ggccagttcc tgccgctggc ctccaggctg cagctcctgg acgacatagc
    11281 gctgcaggtc gtggacgacc tgacccggaa cctgccgcag ctcgacgcca tgtttggcac
    11341 agcgatcaaa tacagcatca acgtctcgcc cgcacaggcc atcgataccg acttcatgcg
    11401 gcggctgacg cagcgcctcc cctccgggca ggcccagcgc ttcatcgtcg agctcaccga
    11461 ggagtcactg gccgacaccg gcctgctgga ggcccatgtg ctgcccatgc tgcgccaggc
    11521 cggggtgcat gtttccattg acgacttcgg caccggctac tcatcgctat cgaaactggc
    11581 gagcctgacg gtggacgagc tcaagatcga ccgttcgctg gtgcagacca tccaacaact
    11641 gccacgcaat cagctgatcc tgcgcgccat agagtcgctg ggcacggcgc tggggctgtc
    11701 catcgtcgcc gaaggcatag aaaccgaaca ggagcgtgac ttcctgctgg ccaacacggc
    11761 catcaccatg ggccagggct ttttgttcca caagccgcaa ttgatcgcca ccctagtggg
    11821 cgaaaccccc tccatgccaa gcccggcgcc agaccggctg gagtcggcct aaa
//
LOCUS       DAOMTQ010000097        11825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_997_length_11825_cov_25.224554, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000097 DAOMTQ010000000
VERSION     DAOMTQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_11825_cov_25.224554"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK518_10195"
     CDS             complement(<1..509)
                     /locus_tag="PK518_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM49327.1"
                     /translation="MEKMKLVLVGNGMAGVRTLEELLKIAPDLYDITIFGAEPHPNYN
                     RILLSPVLAGEQTLEQIVLNDWAWYQEHGITVHAGCTVNEVNRARRTVHASGKDGQAI
                     SVPYDRLILATGSNPFMLPIPGKDLQGVLAYRDIADTQAMIDAATQYRHAVVIGGGLL
                     GLEAANGLM"
     gene            complement(808..2463)
                     /locus_tag="PK518_10200"
     CDS             complement(808..2463)
                     /locus_tag="PK518_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HRM49328.1"
                     /translation="MNGAQALMKTLADAGIAVCFTNPGTSEMHFVAALDSEPRMRAVL
                     ALFEGVATGAADGYARMADKPAATLLHLGCGLGNGLANLHNARKGKVPVVNIVGDHAT
                     THTQYDAQLQSDIETVARNVSPGFVRTSASTEALCQDAVDAVQAARGLPGQVATLILP
                     ADVSWGEGAQPCAPPAVPAAPAADDASVQAIAQLLRSGKKTALLLGGQALRKPGLLAA
                     ARIAAQAGVQLLAEVFPTRLTRGAGLPAVERIAYLAEMAGVQLADLEHLILVDAKAPV
                     SFFAYPGKKSDLVPDGCSVHTLAAPEQDALASLEKLCAALGAQQAQPRLQAPLRPGRP
                     TGRLTAPKVCKAVGHLLPEGAILIDEAITSGLMLGAMTAGCPRHDLITLTGGAIGQGL
                     PNAVGAAIACPDRPVIALIGDGTAMYTIQALWSMAREQLHVVSIIFNNASYSVLNVEL
                     ERVGAGEGGAKARAQLDLAGPRLNFAQLAQGMGVHAVRASTAEDFTQALEYALATPGP
                     HLIEALVPESLSGAKRKLLPWLLKSLPSLPRPVARALKQKIAP"
     gene            complement(2533..4023)
                     /locus_tag="PK518_10205"
     CDS             complement(2533..4023)
                     /locus_tag="PK518_10205"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRM49329.1"
                     /translation="MHNDAQHLSPLPQRPNVPNVPAHIPQPLRDCFAAQHAAFQKQRC
                     PSLAERRADLRALHRLLVDHREALVDAVNRDYGCRSRFETLMTELLQAQEGLLDAIKR
                     LPRWMRREKRSLDISQYPLASAWVQPQPLGVVGVVVPWNFPIAMAVQPMIGALAAGNR
                     VMVKMSENSAQLARLLQEVLPRYFAADKVSVFADRGEDGASIGPLFTQLPFNHLFFTG
                     SPQTGRAVMANCAPNLTPVTLELGGKSPAIVAPGYPLDKAAERILWVKMLNAGQICTN
                     VDYLFVPAGQEQAFIQQAQRVVQERYPDLCNGDYTAIIDQRQYDRLQAMLQDAIAQGA
                     QAVPLAPGQSGDAARRIMPPVALLNVHDGMQVMQREIFGPILPILGYQNRDEVTQYIN
                     DRPHPLALYLFSHERALQQHYLTQTLSGGVTLNDTLLHVGQHHLPFGGVGNSGMGHYH
                     GYEGFLTFSKLRPVFQQGPLRTVNFLQPPYAGLASKMLNIMLWRNR"
     gene            complement(4034..5668)
                     /locus_tag="PK518_10210"
     CDS             complement(4034..5668)
                     /locus_tag="PK518_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRM49330.1"
                     /translation="MEFDYIVIGAGSAGSVVASRLSEDPAVTVCLLEAGGPDSSVLTR
                     APLGFVAEAQLKKNSVHYTTVPQPGLNGRQGFQPRGQVLGGSSAINAMIYCRGHRSDY
                     DRWAAEGNPGWDYASVLPYFKRAEDSECFGATDYHGQGGPLGVAYLRSPFPTSQAFLR
                     ACAAQGLAQTPDYNGAQQEGCWPAQVTQRGGERCSAARAYLTPHLGRSNLTVLTHAQC
                     LRLQWQGRRAEGVHIVRGGQQSLVRARREIVLSGGAYGSPQLLMCSGVGPADQLQAQG
                     IAVVQALTGVGQNLQDHITASLNWKGLRSDAGFGVCPSGAMALLRGMREWQAQRTGCI
                     TSNVAEAGAFFRTEAGLHASDIELEFVAAIVEDHSRKLHWGHGFGLHVTLTRPQSRGE
                     VRLASSDARQSLLIDPRYFSHPDDMPRLVKGVQKSLAIMNDAALAPWRGKMLSPISAD
                     DPAGIERELRRSADTEYHPVGTCRMGPADNPAAVVGPDLRVHGCTGLRVADASVMPNI
                     TTGNTNAPSIMIGEKCADLLRAAARAPARSSAGSFA"
     gene            complement(5724..7334)
                     /locus_tag="PK518_10215"
     CDS             complement(5724..7334)
                     /locus_tag="PK518_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM49331.1"
                     /translation="MTALQADLVVVGGGLAGIVTALEALRAGRSVALVDRDSPERFGG
                     LALWAFGGMALVGTRLQAKKKIPDSPARALADWQRFGELDPNDRWPQAWARYYVEHSA
                     TEVYDWLLQEGVKFMPAVNLVERGRNGEGNSVARYHLLWGTSRCMTLRLIEQLRAAGS
                     GGKLTLLHRHRVTQLEHTDGRVSGALAIDEATGAEVHLRAPAVVLAMGGINGSHEEAR
                     RNWPSDRPQPKTMLNGAHPFADGRLHHLAADQLGAQITHAGEMWNYAAGFPHPFPHFP
                     GHGLSTIPCKSALWLNHRGERIGPEPLVTGFDTHWLCQRVASQEKPWTWHLLNWRIAA
                     KEFAISGAEHNQRIRDRQLLAFLKETLLGNHRLVRQMQQQSPHFLVADSLAELTAKMN
                     ALTASHDIAPAQLQATADAFDANFAPGAKPEDDAQIRRILHARQWGPDRLRTCKPAPL
                     QMRGAGPFIAIQMQLITRKSLGGLQTDLHSRVLDAAEQAIPGLYCVGEAAGFGGGGAS
                     GKRSLEGTFLPGCILTAKAAANHIKNGL"
     gene            7428..8462
                     /locus_tag="PK518_10220"
     CDS             7428..8462
                     /locus_tag="PK518_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM49332.1"
                     /translation="MTLQATVSMGWVGTVLAAAERQGVARGALLAQAGIAPEELTAER
                     WPIDHITRLWRAAVHCTQDAGFGLKAGAGVGPASFNVVSYLLQSAPTLRAALALVQKY
                     QRLISDGGRFQTITGPEACWVVYHPRQGALAFSPHQIESVLAAVVVFARWVTGSALRP
                     QRVQLSQARIGPLAGYREVFQCPVDFEQAFSGVLLANAQLDAPLPQANAQLAQAHQQQ
                     AAARLAALSRQDGLEQTLRMWIASQLQGQAPARAQAARALGLSERTLARRMRAEGLSY
                     SALLDGVRRDAALQAVAQTTRALSDIALALGYAEPSVFTRAFRRWTGATPGQWRGRGA
                     ADMRAQPPAA"
     gene            8524..8898
                     /locus_tag="PK518_10225"
     CDS             8524..8898
                     /locus_tag="PK518_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRM49333.1"
                     /translation="MRALQGMTWLLTLQLVGEALARLLRLPFPGPVVGMLLLVASLHF
                     RVLREPVHAAATYLLSHLSLLFVPVGVGVITHLDLLRDYGVRLMLVIALSTWAGMAVT
                     TLLVRALVRNPQDARKEGSTDA"
     gene            8891..9616
                     /locus_tag="PK518_10230"
     CDS             8891..9616
                     /locus_tag="PK518_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRM49334.1"
                     /translation="MPDFVQLWVYLSSAPLFGLTATLAVYVVAHGLYVRSGQAPWANP
                     VLWSVVVLGTTLMLTKVPYPTYFAGAQFIHFLLGPAVVALGFPLWDRREELARNWGRL
                     LIAALAGGATAAIVAVLAGWAMGLTGEVLRSLAPKSVTAPVAMGVAEKIGGVPALAAV
                     FAVLAGLVGALSAKALFRILGVGTSDADWIARGFGLGTVSHGIGAARALQVNADAGAY
                     AGLALSLQVLLAALLIPLVARYF"
     gene            complement(9876..11279)
                     /gene="lpdA"
                     /locus_tag="PK518_10235"
     CDS             complement(9876..11279)
                     /gene="lpdA"
                     /locus_tag="PK518_10235"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRM49335.1"
                     /translation="MKELTTKLLVIGGGPGGYVAAIRAGQLGIPTVLAEGAQLGGTCL
                     NIGCIPSKALIHAAENFETARHQAAGSQLGIRVADPQIDIAQTVRWKDGIVTRLTGGV
                     GALLRKAGVKVLKGWAQIEDGKTAIVQVDGEAVRVRCEHLLLATGSEPVELPSMPFGG
                     PGGPIWSSTDALSPDILPKRLVVVGAGYIGLELGMAYRKLGVDVTVVEAAQRVLPGYD
                     EELTQPVLDALQKSGIVLHLGCSVQGWDAEHGVHVRSDERAEEFALPAERVLVAVGRR
                     PRTAGFGLESLQLTMAGRHVAIDGHCRTSMRNVWAIGDVTGEPMLAHRAMAQGECVAE
                     QIAGKNRSFAPVAIPAVCFTDPEVVVAGKTPGEARAAGIDCIDAAFPFAANGRAMTLE
                     ATGGFVRVVARKSDHVILGWQAVGRGVSELAAAFGQSLELGARLEDVGHTIHAHPTLG
                     EAVQEAALRALGQALHI"
     gene            complement(11284..>11825)
                     /locus_tag="PK518_10240"
     CDS             complement(11284..>11825)
                     /locus_tag="PK518_10240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRM49336.1"
                     /translation="TRGKLTLLPFLARAMVLALRDFPQINARFDDDAGVVTRHEGVHL
                     GIAAQTDGGLMVPVLRHAESLDLWACAAGIARVAEGAKSGKLGRDELSGSTITLTSLG
                     ALGGIVSTPVINHPEVAIVGVNRMVERPMLRNGQVVARQLMNLSSSFDHRVVDGMDAA
                     QFIQAIRQLLETPALLFVE"
BASE COUNT         1658 a         3975 c         4214 g         1978 t
ORIGIN      
        1 ttcatcaggc cgttggccgc ctccaggccc aacaggccgc cgccaatgac cacggcgtgg
       61 cggtactgcg tggccgcgtc aatcatggcc tgggtgtcgg caatgtcgcg gtaggccagc
      121 acgccctgca aatccttgcc cggaatcggc agcatgaagg ggttggagcc ggtggccagg
      181 atcagccggt catacggcac gctgatggcc tggccgtctt tgccgctggc gtgcacggtg
      241 cgccgcgcgc ggttcacttc gttcacggtg cagccggcgt gtacggtgat gccgtgctcc
      301 tggtaccagg cccagtcgtt gagcacgatt tgctccaggg tttgttcgcc ggcgagcacc
      361 ggcgagagca ggatgcggtt gtagttgggg tgcggctcgg cgccgaagat ggtgatgtcg
      421 tacaggtcgg gcgcgatctt gagcagctct tcgagcgtgc gcacccccgc catgccgttg
      481 cccaccaaca ccagtttcat tttttccatg gctccccctt gccttgcgtg tggatccggc
      541 cagcgggcag tcatgccgca tatggcccat ggccgccatc ggccacgcag ggttcaatgc
      601 aacgaccgtg ccaacgctgg cacagggggc aatggccagc ggctacggtg ttttgccggg
      661 tcttggtgca aggccctggt gcggcgcacc aaaaaaggcc gttggcggcg aagcgtccat
      721 gcaggccatg cctgcggcgc gcgctcgccc ggggtcttgc ccgcgcccac ggcgggcacg
      781 gggcgtgggc atccctatcg ctctcgacta cggtgctatc ttttgcttca gcgcccgcgc
      841 caccggccgc ggcaggctgg gcagcgactt gagcagccag ggcagcagct tgcgcttggc
      901 gccgctgagc gactcgggca ccagcgcctc gatcaggtgc gggccgggcg tggccagggc
      961 gtattccagc gcctgggtga agtcctcggc cgtgctggcg cgcacggcgt gcacgcccat
     1021 gccctgggcg agctgggcga agttcaggcg cggcccggcc aggtcgagct gggcgcgcgc
     1081 cttggcgccg ccttcgcccg cgcccacgcg ttccagctcc acgttcagca ccgagtagct
     1141 ggcgttgttg aagatgatgc tgaccacatg cagctgctcc cgcgccatgc tccacagcgc
     1201 ctggatggtg tacatggccg tgccgtcgcc gatcagcgcg atcaccgggc gatccgggca
     1261 ggcaatggcc gcacccacgg cattgggcag gccctggccg atggcgccgc cggtcagggt
     1321 gatgaggtcg tgacgcgggc agccggccgt catggcgccc agcatcaggc cgctggtgat
     1381 ggcctcgtcg atgaggatgg cgccctcggg cagcaggtgg cccacggcct tgcagacctt
     1441 gggcgcggtc agccggcccg tggggcggcc ggggcgcagc ggcgcctgca gccgtggctg
     1501 ggcctgctgc gcgcccaggg cagcgcagag cttttccagg ctggccagcg cgtcctgctc
     1561 gggcgcggcc agggtgtgca cgctgcagcc gtccggcacc agatcgctct ttttgccggg
     1621 gtaggcaaag aaggacacgg gcgccttggc atcgaccagg atcaggtgct ccaggtcggc
     1681 cagctgcacg ccggccattt cggccaggta ggcgatgcgt tccaccgccg gcaggccggc
     1741 gccgcgcgtc aggcgcgtgg ggaagacctc ggccagcagc tgcacgccgg cctgcgcggc
     1801 gatgcgcgcg gcggccagca ggccgggttt gcgcagcgcc tggccgccca gcagcagggc
     1861 ggtcttcttg ccggatcgca gcaactgggc gatggcctgc acgctggcat cgtcggcagc
     1921 gggcgccgca ggcacggcgg gcggggcgca gggctgggcg ccttcgcccc aggagacatc
     1981 ggccggcagg atcagcgtgg ccacctggcc cggcaggccg cgcgcggcct gcacggcgtc
     2041 caccgcgtcc tggcacagcg cctcggtgct ggcgctggtg cgcacaaagc cgggcgagac
     2101 gttcctggcc acggtttcta tgtcggactg cagctgcgcg tcgtactggg tatgcgtggt
     2161 ggcgtggtcg cccacgatgt tgaccaccgg caccttgccc ttgcgtgcgt tgtgcagatt
     2221 cgccaggccg ttgcccaggc cgcagcccag gtgcaagagc gtggcggcgg gcttgtccgc
     2281 catgcgcgca tagccgtcgg ccgcgcccgt ggccacgccc tcgaacaagg ccagtacggc
     2341 gcgcatgcgt ggctcgctgt ccagggcggc gacgaagtgc atctccgacg tgccggggtt
     2401 ggtgaagcag accgctatgc cggcgtcggc cagggtcttc atcaatgctt gtgcgccgtt
     2461 catggtgaac tcctttttta atagctgcat gcgattgctg gataagcgat agcggctgtt
     2521 tttgttcaaa atttaacggt tgcgccacag catgatgttc agcatcttgc tggccaggcc
     2581 ggcatagggc ggctgcagga agttgacggt gcgcagcggc ccctgttgga acaccgggcg
     2641 cagcttggag aaggtcagga acccctcgta cccgtggtag tggcccatgc cgctgttgcc
     2701 cacgccgcca aacggcaggt ggtgctggcc cacgtgcagc aaggtgtcgt tgagcgtgac
     2761 gccgcccgac agcgtttgcg tcaggtagtg ttgctgcagc gcgcgttcgt ggctgaacag
     2821 gtagagcgcc agcgggtgcg gcctgtcgtt gatgtactgc gtgacctcgt cccggttttg
     2881 gtagccgagg atgggcagga tcgggccgaa gatctcgcgc tgcatgacct gcatgccgtc
     2941 gtgcacgttc aagagcgcca ccggcggcat gatgcgccgg gccgcgtcgc cgctctgccc
     3001 cggcgccagc ggcacggcct gcgcgccctg ggcgatggca tcttgcagca tggcctgcag
     3061 ccggtcgtat tggcgctggt cgatgatggc ggtgtagtcg ccattgcaca ggtcggggta
     3121 gcgctcttgc acgacgcgct gggcctgttg gatgaaggcc tgctcctggc ctgcgggcac
     3181 gaacaggtag tccacattgg tgcaaatctg cccggcgttg agcatcttga cccacaggat
     3241 gcgctctgcg gccttgtcca gcgggtagcc aggcgcgacg atggcgggcg acttgccgcc
     3301 cagctccagc gtgacgggcg tgaggttggg cgcgcagttg gccatcacgg cgcggccggt
     3361 ctgcggcgag ccggtgaaga acaggtggtt gaacggcagc tgggtgaaca gcgggcctat
     3421 gctggcgccg tcttcgccac gatcggcaaa gacgctgacc ttgtcggccg caaagtagcg
     3481 cggcaacacc tcttgcagca ggcgcgcgag ttgtgccgag ttttccgaca tcttcaccat
     3541 cacccggttg cccgccgcca gcgcgccaat catgggctgc acggccatgg cgatggggaa
     3601 gttccagggt acgacgacgc ccaccacgcc cagcggctgg ggttgcaccc aggcgctggc
     3661 cagcgggtac tggctgatgt cgagcgaacg cttctcgcgc cgcatccagc gcggcaggcg
     3721 cttgatggcg tccagcagcc cttcctgcgc ctgcagcagc tcggtcatca gggtctcgaa
     3781 acggctcctg cagccgtagt cgcggttcac agcgtcgacc agggcctcgc ggtggtccac
     3841 cagcaggcgg tgcagggcgc gcaggtcggc acggcgttcg gccaggctgg ggcagcgctg
     3901 cttttgaaag gcggcgtgct gcgcggcgaa gcagtcgcgc aggggctggg ggatgtgggc
     3961 gggcacgttg ggcacgttgg gccgttgagg cagcggggac aggtgctggg cgtcgttgtg
     4021 catgggctgg cctttacgcg aaggaaccgg cggaagagcg ggcgggagcg cgggcggcgg
     4081 cgcgcagcag gtcggcgcat ttctcgccga tcatgatgct gggcgcgttg gtgttgccgg
     4141 tggtgatgtt cggcatgacg gaggcgtcgg ccacacgcag cccggtgcag ccgtgcacgc
     4201 gcagatccgg cccgaccacg gcggcggggt tgtcggccgg ccccatgcgg caggtgccca
     4261 cggggtggta ttcggtgtcg gcactgcgcc gcagctcgcg ctctatgccg gccgggtcgt
     4321 cggcgctgat gggagagagc atcttgccgc gccagggtgc cagcgccgcg tcgttcatga
     4381 tggccagcga cttctgcacg cccttgacca ggcgcggcat gtcgtccggg tggctgaagt
     4441 agcgcgggtc gatcagcagg ctctggcgcg cgtcgctgct ggccaggcgc acctcgcccc
     4501 ggctttgcgg ccgcgtcagc gtgacatgca ggccaaagcc gtggccccag tgcagcttgc
     4561 ggctgtggtc ctcgacgatg gcggcaacga attcgagctc gatgtcgctg gcgtgcaaac
     4621 cggcttcggt gcgaaagaag gccccggcct cggccacgtt gctggtgatg cagccggtgc
     4681 gctgcgcctg ccactcgcgc atgccgcgca gcagcgccat ggcgcccgag gggcagacgc
     4741 caaagccggc gtcgctgcgc aggcccttcc agttcagcga tgcggtgatg tggtcctgca
     4801 ggttctgccc cacgccggtc agcgcctgca ccacggcaat gccctgcgcc tgcagctgat
     4861 cggccgggcc cacgcccgag cacatcagca gctggggcga gccgtaggcg ccgcccgaca
     4921 gcacgatctc gcggcgcgcg cgcaccaggc tttgctggcc gccgcgcacg atgtgcacac
     4981 cctcggcgcg ccggccctgc cactgcaggc gcaggcactg ggcatgggtg agcacggtga
     5041 ggttgctgcg tcccaggtgc ggcgtgaggt aggcgcgcgc ggcactgcag cgctcgccgc
     5101 cgcgctgcgt gacctgggcc ggccagcagc cctcttgctg cgcgccgttg tagtcgggcg
     5161 tctgcgccag gccctgcgcc gcgcaggcgc gcaggaacgc ctgcgaggtg ggaaaagggc
     5221 tgcgcaaata ggccacgccc agcggcccgc cctggccgtg gtagtcggtg gcgccaaagc
     5281 attcgctgtc ttcggcgcgc ttgaaatagg gcagcacgct ggcgtagtcc cagccggggt
     5341 tgccctcggc cgcccagcgg tcgtagtcgc tgcggtggcc gcggcagtag atcatggcgt
     5401 tgatggcgct gctgccgccc agcacctgcc cgcgcggctg aaagccctgg cgcccgttca
     5461 gccccggctg cggcacggtg gtgtagtgca ctgagttctt cttgagctga gcctcggcca
     5521 caaagcccag tggcgcgcgc gtgaggacgc tgctgtccgg cccaccggcc tccagcaggc
     5581 agaccgtgac ggcggggtct tcgctcaggc ggctggccac caccgagccg gccgagccgg
     5641 caccgataac gatgtagtca aactccatgt tgtctcctga attgctttta atttgatagc
     5701 tgtttgcgct ttaccaaaaa gcgctagagt ccatttttta tatgatttgc cgcagccttc
     5761 gccgtgagga tgcagccggg caggaaggtg ccctccagcg accgctttcc gctggcgccg
     5821 ccgccgccaa agcccgccgc ctcgcccacg cagtacaggc cggggatggc ttgttctgcc
     5881 gcatccagca cgcggctgtg caaatcggtc tgcaggccgc ccaggctttt gcgcgtgatg
     5941 agctgcatct ggatggcgat gaacgggccc gcacctctca tctgcagcgg cgcgggtttg
     6001 caggtgcgca gcctgtccgg cccccactgg cgcgcatgca ggatgcggcg gatttgcgcg
     6061 tcgtcctcgg gcttggcgcc gggcgcaaag ttcgcatcaa aagcgtcggc cgtggcttgt
     6121 agctgcgcgg gggctatgtc gtgcgaggcg gtgagcgcgt tcatcttcgc ggtcagctcg
     6181 gccaggctgt cggctaccag gaaatgcggg ctttgctgct gcatctggcg caccagccga
     6241 tggttgccca gcagcgtttc cttgaggaag gccaggagct gccggtcgcg gatgcgctgg
     6301 ttgtgctcgg cgccggagat ggcgaactcc ttggccgcga tacgccagtt gagcaggtgc
     6361 caggtccagg gcttctcttg gcttgccacg cgctggcaca gccaatgcgt gtcaaagccg
     6421 gtgaccaggg gctcgggacc gatgcgctcg ccccggtggt tcagccacag ggccgatttg
     6481 cacggaatgg tggacaggcc atggccgggg aagtgcggga acgggtgcgg aaagcccgcc
     6541 gcgtagttcc acatctcgcc agcgtgggtg atttgtgctc ccagctggtc ggcggccagg
     6601 tggtgcagcc ggccgtcggc aaagggatgc gcgccgttga gcatggtttt gggctgcggc
     6661 cggtcgctgg gccagttgcg ccgcgcctcc tcatggctgc cgttgatgcc gcccatggcc
     6721 agcacgacgg ctggcgcgcg caagtgcacc tcggcacccg tggcctcgtc gatggccagc
     6781 gcgccgctga cccgcccatc ggtgtgttcg agctgcgtca cgcggtggcg gtgcagcagc
     6841 gtcagcttgc cgccgctgcc cgccgcacgc agttgctcaa tcaggcgcag cgtcatgcag
     6901 cgcgaagtgc cccacagcag gtggtagcgc gccacgctgt tgccctcgcc gttcctgccg
     6961 cgctcgacca gattcaccgc gggcatgaac ttcacgccct cttgcagcag ccagtcgtaa
     7021 acctctgtgg ccgaatgctc gacgtagtaa cgcgcccagg cctgcggcca gcggtcgttg
     7081 gggtcgagct caccaaaacg ctgccaatcg gccagcgccc gcgcggggct gtcggggatc
     7141 tttttcttgg cttgcagccg ggtgcccacc agcgccatgc cgccaaaggc ccacagggcc
     7201 aggccgccga aacgctcggg gctgtcgcga tcgaccagag ccacgctgcg cccggcgcgc
     7261 agcgcctcca gcgcggtgac gatgccggcc aggccgccgc ccacgaccac caggtctgcc
     7321 tgcaatgctg tcatgtgttt gtctccctgg ggccagcctg ccccttcttg tggtggcgaa
     7381 gatagccgct aagatggccg ggtgattgac ttttcaggac atcgcacttg actttgcagg
     7441 ccaccgtatc catgggctgg gtgggcaccg tgctggccgc tgccgagcgg cagggcgtgg
     7501 cgcgcggcgc gctgctggcc caggccggca ttgcgcccga agaactgacg gccgagcgct
     7561 ggcccatcga tcacatcacg cggctgtggc gcgccgcggt gcactgcacg caggatgcgg
     7621 gctttggcct gaaggccggc gccggcgtgg ggccggccag cttcaacgtg gtgagctacc
     7681 tgctgcagtc cgcgcccacg ctgcgcgcgg cgctggcgct ggtgcagaag taccagcgcc
     7741 tgatcagcga cggcgggcgc tttcagacca tcaccggccc cgaagcctgc tgggtggtct
     7801 atcacccccg ccagggcgcg ctggccttca gcccgcacca gatcgagtcc gtgctggcgg
     7861 ccgtggtggt gtttgcgcgc tgggtcaccg gcagcgcact gcggccccag cgcgtgcaac
     7921 tgagccaggc gcgcatcggc ccgctggccg gctaccgcga ggtgttccag tgcccggtgg
     7981 acttcgagca ggccttcagc ggcgtgctgc tggccaacgc ccagctcgat gcgcccctgc
     8041 cccaggccaa tgcccagctg gcgcaagcgc accagcagca ggcggccgcg cgcctggcgg
     8101 ccctgtcccg gcaggacggg ctggagcaga cgctgcgcat gtggatcgcc agccagctgc
     8161 aggggcaggc gccggcgcgg gcgcaggcgg cgcgcgccct gggcctgtcc gagcgcaccc
     8221 tggcgcggcg catgcgggcc gaaggcctga gctactccgc gctgctggac ggcgtgcggc
     8281 gcgacgccgc cctgcaggcg gtggcgcaga ccacgcgcgc gctgtccgac attgcgctgg
     8341 ccctgggcta tgccgaaccc agcgtgttca cccgcgcctt caggcgctgg accggtgcca
     8401 cgcccggcca gtggcgcggc cggggggcgg cggacatgcg cgcccagcca ccggccgcct
     8461 gacgattgca gtacaacagc cacccgccgt ctccatccac catctctcaa aggaacccaa
     8521 cccatgcgcg cacttcaagg catgacctgg ctgctgacgc tgcagctggt cggcgaagcc
     8581 ctggcccgtc tgctcaggct gccgtttccc ggccctgtcg tgggcatgtt gctgctggtg
     8641 gcgtcgctgc actttcgcgt gctgcgcgag ccggtgcatg ccgcggccac ctatctgctg
     8701 tcgcacctgt cgctgttgtt cgtgccagtg ggggtgggcg tcatcaccca ccttgatctg
     8761 ctgcgcgact atggcgtcag gctgatgctg gtcattgccc tgtccacctg ggccggcatg
     8821 gccgtcacga cgctgctggt gcgcgcgctg gtgcgcaacc cgcaggatgc acgcaaggag
     8881 ggatctaccg atgcctgatt ttgtccagct ctgggtttac ctctcgtccg cgccgctgtt
     8941 cggcctgacg gccacgctgg ccgtgtatgt ggttgcacac gggctgtatg tgcgctccgg
     9001 ccaggccccc tgggccaatc cggtgctgtg gtcggtggtg gtgctgggca cgacgctgat
     9061 gctcaccaag gtgccctacc ccacctattt tgcgggcgcg cagttcattc acttcctgct
     9121 cgggcccgcg gtcgtggcgc tgggatttcc gctgtgggac agacgcgagg agctggcgcg
     9181 caactggggc cgcctgctga tcgcggcgct ggccgggggc gccacggcgg ccatcgtggc
     9241 ggtgctggcg ggctgggcca tggggctgac gggcgaggtg ctgcgctcgc tggcgcccaa
     9301 atccgtcacc gccccggtgg ccatgggcgt ggccgagaaa atcggcggcg ttccggcgct
     9361 ggcggcggta ttcgccgtgc tggcgggcct ggtcggcgcg ctgtcggcca aggccttgtt
     9421 tcgcattctg ggcgttggca ccagcgatgc cgattggatc gcgcgcggct ttggcctggg
     9481 cacggtgtcg cacggcatag gtgctgcgcg cgccctgcag gtcaatgccg acgccggcgc
     9541 ctacgccggc ctggccttga gtctgcaggt gctgctggcc gcgctgctga taccgctggt
     9601 ggcccgctac ttctagtgca gtgtttgatg cttgatggaa caaataaaac cgatggattt
     9661 ttggtctggg caaggcgcaa accgtagcga tagccgtagc tatcgcgagg atttgcaacg
     9721 ccgcccgggc cgaaaaggcg cggttttatg ttccagatag cgccgaacac tgcactagtg
     9781 atccgcatcc gctgatgcgg ctctcccgca agcggcaatg ggcccatgcc agcgccggtc
     9841 gactgccggc cgaggcgctg gatgcgcagc aggcatcaga tgtgcagcgc ctgccccagc
     9901 gcacgcagcg ccgcctcctg caccgcctcg cccagcgtgg ggtgggcgtg gatggtgtgg
     9961 cccacgtcct ccaggcgcgc gcccagctcc agcgactggc cgaaggcggc ggcgagctcg
    10021 gacacgccgc ggcccacggc ctgccagccg aggatgacat ggtcgctctt cctcgccacc
    10081 acgcgcacga agccgccggt ggcctccagc gtcatggccc ggccgttggc ggcgaacggg
    10141 aaggcggcat cgatgcaatc gatgccggcg gcgcgcgcct cgcccggggt cttgccggcc
    10201 accaccacct cggggtcggt gaagcacacg gcaggaatgg ccacgggggc gaagctgcgg
    10261 ttcttgccgg cgatttgctc ggccacgcat tcgccctggg ccatggcgcg gtgcgccagc
    10321 atgggctcgc cggtcacatc gccaatcgcc cagacattgc gcatcgaggt gcggcaatgc
    10381 ccgtcgatgg ccacatggcg cccggccatg gtcagctgca gcgattcgag gccaaagccc
    10441 gcggtacgcg gcctgcggcc gacggcgacc agcacgcgct cggcgggcag ggcgaattcc
    10501 tcggcgcgct cgtcgctgcg cacatgcacg ccatgctctg catcccagcc ctgcacgctg
    10561 cagcccaggt gcagcacgat gccgcttttt tgcagcgcgt ccagcacggg ttgggtcagc
    10621 tcctcgtcgt agccgggcag cacgcgctgc gcggcctcga ccaccgtcac gtccacgccc
    10681 agcttgcggt aggccatgcc cagctccagg ccgatgtagc cggcgccgac gacgaccagg
    10741 cgtttgggca ggatgtcggg cgagagcgcg tccgtcgatg accagatcgg gcctccaggg
    10801 ccgccaaagg gcatgctggg cagctcgacc ggctcggagc cggtggccag cagcagatgc
    10861 tcgcagcgca cgcgcacggc ctcgccgtcg acctgcacga tggccgtctt gccgtcctcg
    10921 atctgcgccc agcccttcaa gaccttgacg ccggccttgc gcaagagcgc gcccacgccg
    10981 cccgtgaggc gggtgacgat gccgtccttc cagcgcacgg tctgcgcgat gtcgatctgc
    11041 gggtctgcca cgcgtatgcc cagctgtgag cccgccgcct ggtggcgcgc ggtctcgaag
    11101 ttttcagccg catggatcag cgccttggac gggatgcagc caatgttcag gcaggtgccg
    11161 cccagctgcg ctccttcagc cagcaccgtg ggtatgccca actggccggc gcgtatcgcg
    11221 gccacatagc cgccgggacc accgccgatg accagcagtt tggtagtcaa ttccttcatc
    11281 acatcactcc acaaacagca gggccggggt ttcaagtagc tggcgtatcg cctggatgaa
    11341 ctgcgccgcg tccatgccat cgaccacgcg gtggtcgaac gaggacgaca ggttcatcag
    11401 ctggcgcgcc accacctggc cattgcgcag catcgggcgc tcgaccatgc ggttgacgcc
    11461 gacgatggcc acctcggggt ggttgatcac cggggtgctg acgatgccgc cgagcgcccc
    11521 caggctggtc agggtgatgg tggagcccga gagctcgtca cgccccagct tgccactctt
    11581 ggcgccctcg gccacgcggg cgatgccggc ggcgcaggcc cacaggtcca ggctttcggc
    11641 atggcgcagc accggcacca tcaggccgcc atcggtctgc gcggcgatgc ctaagtgcac
    11701 gccctcgtgg cgcgtgacca cgccggcgtc gtcgtcaaag cgcgcgttga tctgcgggaa
    11761 gtcacgcagg gccagcacca tggcgcgggc caaaaacggc agcagcgtga gcttgccgcg
    11821 cgtgg
//
LOCUS       DAOMTQ010000098        11538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1034_length_11538_cov_27.374554, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000098 DAOMTQ010000000
VERSION     DAOMTQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11538
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1034_length_11538_cov_27.374554"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /gene="ccsA"
                     /locus_tag="PK518_10245"
     CDS             <1..492
                     /gene="ccsA"
                     /locus_tag="PK518_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRM49337.1"
                     /translation="WYKLAPLWLLGVVLCFEPIVFRQGATAEAGSSYWMVYFGVSALI
                     VAGILLGRRRIAEGLPALQVLDDVMYKSIAVGFAFFTIATVLGALWAAEAWGGYWSWD
                     PKETWALIVWLNYAAWLHMRLMKGLRGTMSAWWALVGLAVTTFAFLGVNMFLSGLHSY
                     GTL"
     gene            609..>1441
                     /gene="msrP"
                     /locus_tag="PK518_10250"
     CDS             609..>1441
                     /gene="msrP"
                     /locus_tag="PK518_10250"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HRM49338.1"
                     /translation="MRIHSKHSGFDHPRASEITPRAKYEQRRALLRLLAAGAGGAALA
                     SWAGREALAATRPGKLAALEGQRSAVAGAVTMEKPTEYQDATGYNNFYEFGTDKADPA
                     RNAHTLKTRPWTVEVEGLVARPRRFDIDELLKLSPQEERIYRLRCVEGWSMVIPWVGY
                     SLAELIKRVQPLGSAKYVEFVTLADKAVMPGVNGRILDWPYTEGLRLDEAMHPLTLLA
                     FGMYGELLPNQNGAPVRIVVPWKYGFKSAKSIVKIRFTEKEPATAWNKAARNEYGFYS
                     NV"
     assembly_gap    1442..1541
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1544..2107
                     /locus_tag="PK518_10255"
     CDS             1544..2107
                     /locus_tag="PK518_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HRM49339.1"
                     /translation="MHGLCLLPLAWLVYAAAFDHLGANPAEALIRSLGGWTLRLLCLT
                     LAVTPLRQMLGLPALARFRRMLGVYTFCYALLHLLCYSVFDMGLDWGEIARDVAKRPF
                     ILVGMCAFALLLLLAATSFNRAIRWLGARRWQRLHRSVYLIALLALLHFFWMRAGKND
                     FAEVAVYAAIVAVLLLARIGRKLLLKK"
     gene            complement(2159..3451)
                     /gene="lysA"
                     /locus_tag="PK518_10260"
     CDS             complement(2159..3451)
                     /gene="lysA"
                     /locus_tag="PK518_10260"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRM49340.1"
                     /translation="MIPSPLPGQPQLHYRDASLYLEDRRLSDLAREHGTPLYVYSQAA
                     MLQALAAYQRGFAGRKVQICYAMKANSSLAVLQLFARHGCGFDIVSGGELERVMAAGG
                     DPAKVIFSGVGKTRAEMRQALEAGIGCFNVESEAELEVLSAVAQSMGLRAPVSIRVNP
                     NVDAKTHPYISTGLKGNKFGIAHERTVATYQRAAQLPGLRVVGIDCHIGSQITEETPY
                     LDAMDRMLDLVQAVEAAGIAIQHIDFGGGLGINYNGDQPPAADVLWAKLLAKLDARGY
                     GQRQLMIEPGRSLVGNAGVCLTEVLYLKPGEQKNFCIVDAAMNDLPRPAMYQAYHAIV
                     PLEAAGAQQQPQVYDVVGPICESGDWLGRERSLAVAAGDLLAVLSTGAYCSSMGSTYN
                     TRPRPAELLVDGAAAHLIRARESVSDIYRSERLVPTKA"
     gene            complement(3448..3648)
                     /locus_tag="PK518_10265"
     CDS             complement(3448..3648)
                     /locus_tag="PK518_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49341.1"
                     /translation="MLRARQILVRTIALALGAAALGCGQRGPLYLPTEPAAAERATLP
                     QTLNPAATPATPSSAPAPTSKP"
     gene            3661..3990
                     /gene="cyaY"
                     /locus_tag="PK518_10270"
     CDS             3661..3990
                     /gene="cyaY"
                     /locus_tag="PK518_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HRM49342.1"
                     /translation="MTDLEFLDRAEQLLLAVEQGCDRINDATDADLDSQRSGGMVSIS
                     FANGSQIIINLQKPLHEVWLAAQSGGYHYRFDGQHWMDTKGDGEFFAALSRDASAQSG
                     QALRFTA"
     gene            complement(4007..6388)
                     /locus_tag="PK518_10275"
     CDS             complement(4007..6388)
                     /locus_tag="PK518_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRM49343.1"
                     /translation="MPPSRDKSPAPKPATHSPGWLRWPLRVLAWLIGLGAAAALAAVL
                     VVGVGLAMAYPNLPDVSDLADYRPKLPLRVYSTEGALLGEFGEERRNLTPIEDIPPVM
                     TNAVLAIEDARFFEHGGVDYKGVLRAALANLGRAKSQGASTITMQVARNVYLSSEKTL
                     TRKIYEILLTFKLEHLLSKNQILEIYMNQIYLGNRAYGFAAASEAYFGKPLKSISIAE
                     AAMLAGLPKAPSAYNPISNPKRARIRQLYIIERMQDNGFITAEQAAAAKQEDLKLRSS
                     TNNTRVHAEYVAEMARQLIFAQYGAEAYTRGLNVYTTLNAGEQDAAYSALRKGIMDYE
                     RRQHYRGPERFVTLPTSAAEVEDAIDDALANHPDNGDVLAAVVLEAIPKRILAARADG
                     EPIEITGEGLKPAQSGLSDKAPPNIKLRRGAIIRVVQTPKKTWEITQLPEVEGAFVAI
                     DPRTGAIRALVGGFDFDKNKFNHVTQAWRQPGSAFKPFIYSAALEKGFTPATVINDAP
                     LFFSAGATGAQPWEPKNYDGKYDGPMTMRTGLARSKNMISIRVLQAVGPKTAQEWVSR
                     FGFEADKHPAYLTMALGAGSVTPMQMATAYAVFANGGYRVNPYLITRVVDQKGRILSQ
                     FEPPATSNNPRAIDARNAFVMDSLLQTVTRSGTAARAQATLKRPDLYGKTGTTNDSVD
                     AWFAGFQPTMTAVTWIGYDQPRNLGSRETGGGLALPIWIAFMDKALKGVPVAEPTVPP
                     GVVNVSGEWLYEEYAHNSGVANLGLDGQAPGATAPGPSPAPEERNRILDLFRN"
     gene            6651..7730
                     /locus_tag="PK518_10280"
     CDS             6651..7730
                     /locus_tag="PK518_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRM49344.1"
                     /translation="MVSMGSLFSRQQAPLLGVDISSSSVKLVELGRDKSGALVLECCA
                     IEQLERGWITDGNIEKFDEVAEALRRVVKKSGTRTRNVALALPPSAVITKRISLPGGM
                     TEQELEVQVESEANQYIPFSLDEVSLDFCVVGPSKNASGDVDVLIAASRKEKVQDRQG
                     LAEAAGLKPVIVDIESHAARLAAGRLIEALPNRGVDSVVALFEVGALTTSMQVIRNDD
                     VLYERDQAFGGAQLTQLIVRQYGFSQEEAEGKKRNGDLPEDYESAVLRPFVDSLAQEI
                     GRALQFFFTSTPYHRVDHIMLAGGSAPLHGLTQAVTENTGFACSIINPFEGMDVGGAV
                     RLRKMAREAPSYLTSCGLAMRRFLQ"
     gene            7727..8335
                     /locus_tag="PK518_10285"
     CDS             7727..8335
                     /locus_tag="PK518_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRM49345.1"
                     /translation="MILINLLPHREAARKRRRETFQAIMVASALAGLAIAGLIYWWFQ
                     MQITDQQDKNNFLRGEIQVLEKQIKEIATIEEEIAALQARQKAVEDLQSDRNLPVHLL
                     NELVKQLPDGVYVTSLKQADQVVTMQGMAQSNERVSEMLRNLTNNTPWFSKPELVEII
                     AANVSLSPKDQRRVASFNLRFRLVRSSEAQKADAANEVVAGK"
     gene            8338..9015
                     /locus_tag="PK518_10290"
     CDS             8338..9015
                     /locus_tag="PK518_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRM49346.1"
                     /translation="MAKKKAPKYDFAKLQGDLQRQFQNLDPKDPSLWPALPRTLLCLL
                     IAGGVATFLWFFKLSEYEAELTTERGTEQTLRADYQKKLIKAVSLDALKKQREQVQQY
                     VIQLEKQLPSKAEMSALLSDINQAGLGRSLQFELFRPGQVVVKDYYAELPIAIRVVGK
                     YHDVGAFASDVANLSRIVTLNNVSLAPASKDAQGTLTMEATARTFRYLDPEEIQAQKA
                     AAKGAKK"
     gene            9012..9569
                     /locus_tag="PK518_10295"
     CDS             9012..9569
                     /locus_tag="PK518_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HRM49347.1"
                     /translation="MKRLGCKPLIALLGALLLAGCGGNDEDELRQWMSDLRATTKPRV
                     TPLTEPKQFHPQDYTGDGNMEPFNPQKLTQALRRESSETVANASLIAPEMARRKEPLE
                     SHPLDTVKMVGSLNKTGVPTALVSVDKLLYQVRMGNYLGQNYGKIIGISETNIRLREI
                     VQDSTGDWVERMTTLDLQEGNEVRK"
     gene            9566..>11538
                     /gene="pilQ"
                     /locus_tag="PK518_10300"
     CDS             9566..>11538
                     /gene="pilQ"
                     /locus_tag="PK518_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HRM49348.1"
                     /translation="MIQQQSRLRRHLWLAGIAIAAALSATVSHARGSIESVTGFLQGG
                     AEVLRIEFSEPQTELPTGFAVQTPARIALDFPGVTNASGRSLVEINQGNVKSAHIVEA
                     GERSRIVLNLKQATTYRAELHGKAVLVLLDSVTSAARVPAGPSAVFAEGHNADVLPLK
                     DLDFRRGSDGAGRIVVGLANNQVGVDLKTQGKGLVVEFQRSSLPEGLRRRLDVSDFGT
                     PVQTITAAQQGERVRLTIDPVGDWEHSAYQSDNQFVVEVRPKKVDLSKLTQGPNYSGE
                     KLSLNFQNIEVRSLLQVIADFTNFNIVTSDTVTGALTLRLKDVPWDQALQIIMDAKGL
                     GMRKSGTVLWIAPKDEIDERTRKDFEAAQAIAKLEPLRTQAYQLNYAKAADMVVQLTT
                     NSSSGGGSGSSQNTRFLSERGSAIAEPRTNQLFVTDTPAKLEDVRQLLLTLDVPVRQV
                     MIEARIVEARDTFGRSLGVRLGGGDLRANRGGDGGFNIGGGNRATWGTDYSNATNSSG
                     FGGPVNVGGNFVNLPASLSSGQAVGSFALSIFNSAANRFLTLELSAMEADGQGKVVSS
                     PRLITADQTKALIEQGTEYPYTVTAPNGATTIAFKKAVLKLEVVPQITPEGNIILDLD
                     VNKDSRGESTTQGVAIDTKHIKTQVLVENGGTVV"
BASE COUNT         1990 a         3647 c         3811 g         1990 t
ORIGIN      
        1 tggtacaagc tggcgccgct gtggctgctg ggcgtggtgc tgtgcttcga gcccatcgtc
       61 ttccgccagg gcgcgacggc cgaggccggc agcagctact ggatggtgta ctttggcgtc
      121 tcggcgctga tcgtcgccgg catattgctg ggccgccggc gtatcgccga ggggctgccg
      181 gcgctgcagg tgctggacga cgtgatgtac aagtccatcg ccgtgggctt tgccttcttc
      241 accatcgcca ccgtgctggg cgcgctgtgg gcggccgagg cctggggcgg ctactggagc
      301 tgggacccca aggagacctg ggcgctgatc gtctggctca actacgccgc ttggctgcac
      361 atgcgcctga tgaagggcct gcgcggcacc atgtccgcct ggtgggccct ggtgggcctg
      421 gcggtcacca ccttcgcctt cctgggcgtg aacatgttcc tgagcgggct gcacagctac
      481 ggcacgctgt aaaaacagga gctgcgtgcg cgctcatatg aacgttttca atctgtttat
      541 tggttgaaag ccaggaaaag agagcgcaga cagctattct tttagcgaaa gtcaatggag
      601 gacacaccat gcgcatccat agcaagcata gcggtttcga ccacccgcgc gccagcgaga
      661 tcacgccgcg cgccaaatac gaacagcgcc gcgccctgtt gcgcctgctg gcggcgggcg
      721 ccggcggggc ggcgctggcc tcatgggctg ggcgcgaggc gctggccgcc acgcggccgg
      781 gcaagctggc ggccctggag ggccagcgct cggcggtggc cggcgcggtg accatggaga
      841 aacccaccga gtaccaggac gccacgggct acaacaactt ctacgagttc ggcaccgaca
      901 aggccgaccc cgcgcgcaac gcccacaccc tgaagacccg gccctggacg gtggaggtgg
      961 agggtctggt ggccaggccg cggcgctttg acatcgacga actgctgaaa ctcagcccgc
     1021 aggaggagcg catctaccgc ctgcgctgcg tggagggctg gtccatggtc atcccctggg
     1081 tgggctattc gctggccgaa ctcatcaagc gggtgcagcc gctgggcagc gccaaatacg
     1141 tggagttcgt caccctggcc gacaaggccg tcatgcccgg ggtgaacggc cgcatcctcg
     1201 actggcccta caccgagggc ctgcgcctgg atgaggccat gcacccgctc acccttctgg
     1261 ccttcggcat gtatggcgag ctgctgccca accagaatgg cgcgccggtg cgcatcgtcg
     1321 tgccctggaa gtacggcttc aagagcgcca agagcatcgt caaaatccgc ttcaccgaga
     1381 aagaacccgc cactgcctgg aacaaggccg cgcgcaacga gtacggcttt tattccaacg
     1441 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgctgcatg ggctgtgcct
     1561 gctgccgctg gcctggctgg tgtatgcggc ggctttcgac cacctgggcg ccaacccggc
     1621 cgaggcgctg atccgctccc tgggcggctg gacgctgcgc ctgctgtgcc tgaccctggc
     1681 cgtgaccccg ctgcgccaga tgctggggct gcccgcgctg gcgcgctttc ggcgcatgct
     1741 gggcgtctac accttctgct atgccctgct gcacctgctg tgctacagcg tattcgacat
     1801 ggggctggac tggggcgaaa tagcgcgcga cgtggccaag cggcccttca tcctggtggg
     1861 catgtgcgcg tttgccctgc tgctgctgct ggccgccacc tcgttcaacc gcgccatacg
     1921 ctggctgggc gcgcgccgct ggcagcggct gcaccgcagc gtgtacctga tcgcgctgct
     1981 ggcgctgctg cacttcttct ggatgcgtgc cggcaagaac gattttgccg aggtggctgt
     2041 gtatgcagcc atcgtcgccg tgctgctgct ggcgcggata gggcgtaaac tactattaaa
     2101 aaaatagctg tcagcgctta ttgggtaagc gctgacagcc gttttgtgtc aatgtcgttc
     2161 aggccttggt gggcaccagc ctctcgctgc ggtagatgtc gctgacactc tcgcgcgcgc
     2221 ggatcaggtg cgccgccgcg ccatcgacca gcagctcggc cgggcgcggc cgggtgttgt
     2281 aggtgctgcc catggagctg caataggccc cggtggagag cacggccagc aggtcgcctg
     2341 cggccacggc caggctgcgc tcgcgcccaa gccagtcgcc gctctcgcag atcggtccga
     2401 ccacgtcgta gacctgcggc tgttgctggg cgccggcggc ctccagcggc acgatggcgt
     2461 ggtaggcctg gtacatcgcc gggcgcggca ggtcgttcat ggcggcgtcg acgatgcaga
     2521 aattcttctg ctcgccgggc ttgaggtaca gcacctcggt caggcacacg ccggcattgc
     2581 ccaccaggga acggcccggc tcgatcatca gctggcgctg gccatagccg cgcgcatcca
     2641 gcttggccag cagcttggcc cacaggacat cggccgcggg cggctggtcg ccgttgtagt
     2701 tgatccccag gccgccgccg aagtcgatgt gctggatggc gatgcccgcc gcctccaccg
     2761 cctgcaccag gtccagcatg cgatccatgg cgtccaggta gggcgtctct tcggtgatct
     2821 gcgagccgat gtggcagtcg atgccgacca cgcgcaggcc gggcaactgc gcggcgcgct
     2881 ggtaggtggc cacggtgcgc tcgtgggcga tgccgaactt gttgcccttc aggcccgtgg
     2941 agatgtaggg gtgggtcttg gcgtccacgt tcgggttcac gcggatgctg accggcgcgc
     3001 gcaggcccat ggactgggcc accgcgctga gcacctccag ctcggcctcg ctctcgacgt
     3061 tgaagcagcc tatgccggcc tccagcgcct ggcgcatctc ggcgcgggtc ttgcccacgc
     3121 ccgagaagat gaccttggcc gggtcgccgc cggcggccat cacgcgttca agctcgccac
     3181 ccgagacgat gtcgaagccg cagccatggc gcgcaaacag ctgcagcacg gccagtgatg
     3241 aattggcctt catggcgtag cagatctgca ccttgcgccc ggcaaagccg cgctggtagg
     3301 cggccagggc ctgcagcatg gcggcctgcg aatagacgta cagcggcgtg ccatgctcgc
     3361 gcgccagatc cgacaggcgt cggtcttcga ggtacaggga tgcgtcgcgg taatgcagct
     3421 ggggctggcc gggcaggggg ctggggatca tggtttggag gttggtgcgg gtgcggacga
     3481 tggcgtggcc ggcgtggccg ccgggttgag tgtctgtggc agggtggcac gctcggctgc
     3541 cgccggttcg gtcggcaggt acagcgggcc gcgctggccg cagcccaggg ccgccgcgcc
     3601 aagcgcaagg gcaatcgtcc tgactagaat ttggcgagct ctcaacattc gcaaattgta
     3661 atgaccgacc ttgaattcct ggatcgtgcc gaacaacttc tgttggcggt cgagcagggc
     3721 tgcgaccgca tcaacgacgc caccgatgcc gacctggaca gccagcgctc gggcggcatg
     3781 gtcagcatca gctttgccaa cggcagccag atcatcatca acctgcaaaa accgttgcac
     3841 gaggtctggc tggccgcgca gtcgggcggc taccactacc gcttcgatgg ccagcattgg
     3901 atggacacca agggcgatgg cgagttcttt gcggccttga gccgcgatgc cagcgcccag
     3961 tcgggccagg cgctgcgctt cacggcctga tggccgggcc gcagcatcaa ttgcggaaca
     4021 gatccaggat gcggttgcgc tcctcgggcg cggggctggg gccgggggcg gtggcgcccg
     4081 gggcctggcc atccaggccc aggttggcaa cgccgctgtt gtgggcatat tcctcgtaga
     4141 gccattcgcc actgacgttg accacgcccg gggggacggt gggctcggct accggcacgc
     4201 ccttgagcgc cttgtccatg aaggctatcc agatcggcag cgccaggccg ccgcccgtct
     4261 cgcggctgcc caggttgcgt ggctggtcat agccaatcca ggtgacggcg gtcatcgtgg
     4321 gctggaagcc cgcgaaccag gcgtccaccg aatcgttggt ggtgccggtc ttgccgtaca
     4381 ggtcagggcg cttgagcgtg gcctgcgcgc gtgccgccgt gccgctgcgc gtcaccgttt
     4441 gcagcaggct gtccatgacg aaggcgttgc gtgcgtcgat ggcacgcgga ttgttgctgg
     4501 tggcgggcgg ctcgaactgc gacaggatgc gccccttctg atcgaccacg cgcgtgatca
     4561 gatacgggtt gacgcgatag ccgccgttgg caaacaccgc ataggccgtg gccatctgca
     4621 ttggtgtaac cgagcccgcg cccagggcca tggtcaggta ggcggggtgc ttgtcggcct
     4681 caaagccaaa ccggctgacc cattcctgcg ccgtcttcgg gcccacggcc tgcagcacgc
     4741 ggatcgagat catgttcttc gagcgcgcca ggccggtgcg catggtcatc gggccgtcgt
     4801 acttgccgtc gtagttcttg ggctcccagg gctgggcgcc ggtggcgccg gcgctgaaga
     4861 acagcggcgc gtcgttgatc accgtggccg gcgtgaagcc cttttcgagg gcggcggaat
     4921 agatgaaggg cttgaaggcc gagcccggct ggcgccaggc ctgggtgaca tggttgaact
     4981 tgttcttgtc gaagtcgaag ccgccgacca gcgcgcggat cgcgccggtt cgcgggtcaa
     5041 tggccacgaa ggcgccttcg acctcgggca gctgggtgat ctcccaggtt ttcttcggcg
     5101 tctgcaccac gcggatgatg gcgccgcggc gcagcttgat gttgggcggc gccttgtccg
     5161 agaggccgga ctgcgcgggc ttcagaccct cgccggtgat ctcgatgggc tcgccgtcgg
     5221 cacgagccgc cagaatccgc ttcgggatgg cctccagcac cacggccgcc agcacatcgc
     5281 cgttgtcggg gtggttggcc agggcgtcgt cgatggcgtc ctcgacctcg gcggccgaag
     5341 tcggcagggt cacgaagcgc tccgggccgc ggtaatgctg gcgccgctcg tagtccatga
     5401 tgcccttgcg cagggcggag taggccgcat cctgctcacc tgcattcagc gtggtgtaga
     5461 cgttcaggcc gcgtgtatag gcctcggcgc cgtactgggc aaagatcagc tgacgcgcca
     5521 tctcggccac atattcggca tgtacgcgcg tgttgttggt ggacgagcgc agtttcaggt
     5581 cttcctgctt ggccgcggcg gcctgctcgg cggtgatgaa accgttgtcc tgcatgcgct
     5641 cgatgatgta gagctggcgt atgcgcgcgc gcttggggtt gctgatcggg ttgtaggccg
     5701 acggcgcctt gggcaggccg gccagcatgg ccgcctcggc aatggagatg gatttcagcg
     5761 gcttgccaaa ataggcctcg gaggcggcgg cgaagccgta ggcgcgatta cccagataaa
     5821 tctggttcat gtagatctcg agaatctggt ttttgctcag cagatgctcg agtttgaacg
     5881 ttaacaatat ttcatagatt tttcgggtca acgtcttttc cgacgacaga tagacgttgc
     5941 gcgccacctg catggtgatg gtcgacgctc cctggctctt ggcgcgcccc aggttggcca
     6001 gggcggcgcg cagcacgccc ttgtagtcca cgccgccatg ctcgaagaag cgcgcgtcct
     6061 cgatggccag cacggcattg gtcatcaccg gcggaatgtc ttctatcggc gtgaggttgc
     6121 gccgctcctc gccaaactcg cccagcagcg cgccctcggt ggaatacacg cgcagcggca
     6181 gcttggggcg gtagtccgcc agatcggata cgtcgggcag gttggggtag gccatggcca
     6241 gcccgacccc aaccaccagc acggcggcca gggccgcagc tgcccccagg cctatgagcc
     6301 aggccagcac gcgcagcggc cagcgcagcc aacccggtga atgggtggcg ggcttggggg
     6361 cgggggactt gtctcgtgaa ggcggcatag ggctcaatcg gcaggtaacc cgccattata
     6421 aaaattcagc aaccgctccc tgttacggaa cagtgttggc tggcactcac ttgagcagac
     6481 tggcaagccg tcaggaaatt ccctctgttg ctagaggcat cgtcggcttt tgacaacggg
     6541 tcagatgtat caatcgggtg aaactctttc ctggtgtagg aacatgctga taggattcat
     6601 gtgaagtgtt aagttttgtt gcgtcataac ggtcagtttc ggggggagcc gtggtctcaa
     6661 tggggtcgtt gttcagtcgc cagcaggccc cattgcttgg ggtcgacatc agctcatcca
     6721 gcgtcaagct ggtggagttg gggcgtgata agagtggtgc tctggtgctt gaatgctgcg
     6781 ccatcgagca actggagcgt ggctggatca ccgacggcaa catcgaaaaa ttcgatgaag
     6841 tcgccgaggc cttgcgtcgt gtggtgaaaa aaagcggtac ccgcacgcgc aacgtggcgc
     6901 tggcgctgcc gccgtccgcg gtgatcacca agcgcatcag cctgcccggc ggtatgaccg
     6961 agcaggagct ggaggtgcag gtcgagtccg aggccaacca gtacatcccg ttctcgctcg
     7021 atgaagtgag cctggacttc tgcgtcgtcg gcccgagcaa gaacgcctcc ggcgacgtgg
     7081 atgtgctgat cgccgcttcg cgcaaggaaa aggtgcagga tcgccagggc ctggccgagg
     7141 ctgccggcct caagcccgtc atcgtggaca tcgaatccca tgccgcccgc ctggccgccg
     7201 gccgcctgat tgaggccttg cccaaccgcg gcgtggattc tgtggtggcc ttgttcgagg
     7261 tcggggcgct gaccaccagt atgcaggtca ttcgcaacga tgatgtgctc tacgagcgcg
     7321 accaggcctt cggcggcgcg cagctgacgc agctgatcgt gcgccagtac ggcttttcgc
     7381 aggaagaggc cgagggcaaa aagcgcaacg gcgatctgcc cgaggactat gaatcggccg
     7441 tgctgcgccc cttcgtggac agcctggcgc aggaaatcgg ccgcgccctg cagttcttct
     7501 tcaccagcac gccctaccac cgtgtcgatc acatcatgct ggcgggcggc tcggcaccgc
     7561 tgcacgggct gacccaggcc gtgaccgaga acaccggctt tgcctgcagc atcatcaatc
     7621 cgtttgaggg catggacgtg ggcggcgcgg tgcgcctgag aaaaatggcg cgcgaggcgc
     7681 cgtcgtatct gacgtcctgc ggcctggcga tgcggaggtt cctgcagtga ttttgatcaa
     7741 cctgctcccg caccgggagg ctgcgcgcaa acgccggcgc gagacgttcc aggccatcat
     7801 ggtggcctct gccctggcgg gtctggccat tgccgggctg atctactggt ggttccagat
     7861 gcagatcacc gatcagcagg acaagaacaa cttcctgcgc ggcgagatcc aggttctgga
     7921 gaagcagatc aaggaaatcg ccaccatcga ggaggaaatc gcggctctgc aggcgcgcca
     7981 gaaggccgtg gaagacctgc agtccgaccg caacctgccg gttcacctgc tcaacgagct
     8041 ggtcaagcag ctgcccgatg gcgtctatgt gaccagcctc aagcaggccg atcaggtggt
     8101 caccatgcag ggcatggcgc agtccaacga gcgggtctcc gagatgctgc gcaacctgac
     8161 caacaacacc ccctggttct ccaagcccga gctggtggaa atcattgccg ccaatgtgtc
     8221 cttgtcgccc aaggatcagc gccgcgtcgc gtccttcaat ctgcgcttcc gcctggtgcg
     8281 cagttccgag gcgcaaaagg cggacgctgc gaatgaagtc gtcgcaggga aatagccatg
     8341 gccaagaaaa aagcgccgaa gtacgacttc gccaagctgc aaggcgattt gcagcgccag
     8401 ttccagaacc tcgatccgaa agatccatcg ctgtggccag ccttgccgcg gacgctgctg
     8461 tgcctgctga tcgccggggg tgtggcaacg tttctgtggt tcttcaagct ctctgaatat
     8521 gaggccgagc tgaccaccga gcgcggcacc gagcaaacct tgcgtgccga ctatcaaaag
     8581 aagctcatca aggccgtgag cctggatgcc ctgaagaagc agcgcgaaca ggttcagcaa
     8641 tacgtcatcc agctcgagaa gcagctgccc agcaaggccg agatgtcggc gctgctgtcc
     8701 gacatcaacc aggccggcct gggccgcagc ctgcagttcg agctgttccg cccggggcag
     8761 gtggtggtca aggattacta cgccgagctg cccatcgcca tccgcgtggt cggcaagtac
     8821 cacgacgtgg gcgcattcgc gtctgacgtg gccaacctct cgcgcatcgt caccttgaac
     8881 aatgtctcgc tggcaccggc cagcaaggac gcgcagggca ccctgaccat ggaggccacg
     8941 gccagaacct tccgctatct cgaccccgaa gagatccagg cccagaaggc cgcggcgaaa
     9001 ggagcgaaga aatgaagcgc cttggctgca aacccctgat cgcgctgctg ggcgccttgc
     9061 tgctggcggg ctgtggcggc aatgacgagg acgagctgcg ccagtggatg agcgacctgc
     9121 gcgcgacgac caagccgcgc gtcacgccgc tcaccgagcc caagcagttc catccgcagg
     9181 actacaccgg cgacggcaac atggagccgt tcaacccgca gaaactgacg caggccctgc
     9241 gccgcgagtc gtccgagacc gtcgccaacg cgtcgctgat cgcccccgag atggccagac
     9301 gcaaggagcc gctggagtcc cacccgctcg ataccgtgaa gatggtcggt agcctgaaca
     9361 agactggcgt tccgaccgcg ctggtgagcg tggacaagct gttgtaccag gtgcgcatgg
     9421 gcaattacct gggccagaac tacggaaaaa tcatcggcat ttcggaaacg aatatccgtt
     9481 tacgcgaaat agtccaggac tccacgggcg attgggtgga gcgcatgaca acgctggacc
     9541 tgcaagaggg aaatgaggtg aggaaatgat tcaacagcaa tcgcgcttgc gccgccatct
     9601 gtggctggcc ggcatcgcca tagcggcggc cctgagcgcc acggtatcgc atgcccgggg
     9661 ctccatcgag tcggtaaccg gcttcctgca gggcggtgcc gaggtgctgc gtatcgagtt
     9721 ttccgaaccc cagaccgagc tgcccaccgg ctttgccgtc cagacccccg cgcgcatcgc
     9781 cctggacttc cccggcgtta ccaatgcatc gggccgttcg ctggtggaga tcaaccaggg
     9841 caatgtcaag tcggcccata tcgtcgaggc cggcgagcgc tcgcgcatcg tcctgaacct
     9901 gaagcaggcc acgacctacc gcgcagaact gcacggcaag gcggtgctgg tgctgctcga
     9961 cagcgtcacc agtgccgccc gcgtccccgc cgggccgtcg gccgtgtttg ccgaaggcca
    10021 caacgccgac gtgctgccgc tgaaggatct ggacttccgc cgcggctcgg atggcgccgg
    10081 ccgcatcgtg gtcggcctgg cgaacaacca ggtgggcgtc gatttgaaga cccagggcaa
    10141 gggcctggtg gttgaattcc agcgctcatc gctgcccgaa gggctgcgcc ggcgcctgga
    10201 cgtgtccgac tttggcacgc ccgtgcagac catcaccgcc gcccagcagg gcgagcgcgt
    10261 gcgcctgacc atagaccccg ttggcgactg ggagcacagc gcctaccaga gcgacaacca
    10321 gttcgtcgtc gaagtgcgcc ctaagaaggt ggacctgtcc aagctcaccc agggccccaa
    10381 ctacagcggc gaaaagctgt cgctgaactt ccagaacatc gaggtgcgct cgctgctgca
    10441 ggtgatcgcc gacttcacca acttcaacat cgtcacctcc gacaccgtca ccggcgcgct
    10501 gaccctgcgc ctgaaggacg tgccgtggga tcaggccttg cagatcatca tggacgccaa
    10561 gggcctgggc atgcgcaaat cgggcaccgt gctgtggatc gcgcccaagg acgaaatcga
    10621 tgaacgcaca cgcaaggatt tcgaggccgc ccaggccatc gccaagctcg aacccctgcg
    10681 cacccaggcc taccagctga actacgccaa ggccgccgac atggtggtgc agctgaccac
    10741 caacagctcc tcgggtggcg gcagcggcag cagtcagaac acgcgattcc tgtccgaacg
    10801 cggcagcgcc attgccgagc cgcgtaccaa ccagctgttc gttaccgaca cccccgccaa
    10861 gctggaggac gtgcgccagc tgctgctgac gctggatgtg ccggtgcgcc aggtgatgat
    10921 agaggcgcgc atcgtcgagg cacgcgacac ctttggccga tcgctgggcg tgcgcctggg
    10981 cggtggcgac ctgcgcgcca accgcggggg cgatggcggc ttcaacatcg gtggcggcaa
    11041 ccgcgccacc tggggtaccg actattccaa cgccaccaac tccagcggct ttggcggccc
    11101 ggtgaatgtc ggcggcaatt tcgtgaacct gccggcatcg ctgtccagcg gccaggccgt
    11161 gggctcgttc gcgttgtcca tcttcaactc tgccgccaac cgcttcctga cgctggagct
    11221 gtcggccatg gaggccgatg gccagggcaa ggtggtctcc agcccgcgcc tgatcacggc
    11281 cgaccagacc aaggcgctga tcgagcaggg taccgagtac ccctacaccg tcaccgcccc
    11341 caacggcgcc accaccatcg ccttcaagaa ggccgtgctg aagctggaag tggtgccgca
    11401 gatcacgccc gagggcaaca tcatcctgga cctggacgtc aacaaggaca gccgcggcga
    11461 gagcaccacc cagggcgtgg ccatagacac caagcacatc aagacccagg tgctggtgga
    11521 aaacggcggc accgtggt
//
LOCUS       DAOMTQ010000099        11414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1055_length_11414_cov_26.221498, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000099 DAOMTQ010000000
VERSION     DAOMTQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1055_length_11414_cov_26.221498"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK518_10305"
     CDS             complement(<1..614)
                     /locus_tag="PK518_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM49349.1"
                     /translation="MPQPPCACTSIDELAAALQSVGYFADRRLATAVFLALKLQRPLL
                     LEGEPGVGKTELAKALAQVLSRQLLRLQCFDGLEQREALYEWNYAAQLLHLRAAEGTA
                     SANALEQEVYQPRYLVRRPLLQALEAPAPGALLLIDEVDRADEPFEAFLLEYLGEYQV
                     SIPELGTISAAVPPVTILTSNRTRELNDAVKRRCLYHWLDYPER"
     gene            complement(827..1618)
                     /locus_tag="PK518_10310"
     CDS             complement(827..1618)
                     /locus_tag="PK518_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRM49350.1"
                     /translation="MYAFTYERPASQADALKLAQAGGKLLAGGQTLLASMKLRLAAPE
                     QLVDLGAIPELKGIRREGNSIVIGAMTCHADVAESAELRAAIPALAALAGGIGDKQVR
                     ARGTLGGSVANNDPAADYPAALLALGATVHTTARAIAADDFFQGLFATALEEGELITA
                     VSFPIPKKAAYVKFVQPASLFALIGVFVAQTDGGARVAVTGGGNGVFRHAGLEAALSA
                     SFTPDAVAGVATDEGEMSSDLHGSAAYRAHLVGVMAQRAVAQALA"
     gene            complement(1632..4004)
                     /locus_tag="PK518_10315"
     CDS             complement(1632..4004)
                     /locus_tag="PK518_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRM49351.1"
                     /translation="MGANAFAKLPHIGESLKRKEDVRFLTGVGNYTDDVDQANQKYAY
                     FVRSPHAHARVKGVDTAEAAKMPGVAAIYSGKDIEGKMGGLPCGWLISNPDGSPMKEP
                     MHPILAIGKVRYVGDHVAMVVADTLEQARNAAEAVQVDYEELAPVIDMKKAKDGPALH
                     DEAPDNHCYKWTLGDKAAVDAAFAGAAHVSKLDLVNNRLIPNAIEPRAANASYNRATD
                     EYVLYVANQNPHVERLLLTAFVLQLPEHKVRVIAPDVGGGFGSKIFLYAEDVAVTWAA
                     KQLNCAVKWTAERGESFVSDAHGRDHITHAEMAMDSQGKFLAMRVHTDANLGAYLSTF
                     ASSVPTILYGTLLAGQYTAPQIYVEVDGWFTSTAPVDAYRGAGRPEATYVVERLVTRC
                     AWDLGLSQDEIRKRNFITSFPYQTPVALQYDIGDYHACMNQAQQLADVAGFDARRKAS
                     EARGLKRGMGYSSYIEACGLAPSNIAGALGARAGLFEAGEVRVHPTGSVTVFTGSHSH
                     GQGHETTFAQLVAARLGLSPDQVDIVHGDTGRVPFGMGTYGSRSLSVGGTAIMKALDK
                     IEAKAKKIAAHLMEASDADIEFAGGEFTVKGTDKKIPFAQVALTAYVPHNYPLDKLEP
                     GLNETAFYDPVNFTFPAGTYICEVEVDPQTGVVRVDRFTAVDDFGVIINPMIVEGQVH
                     GGLVQGIGQALLEHGVYDPDSGQLLTGSYMDYAMPRAANFPEFKLGHVCTPCTHNPIG
                     SKGCGEAGAIGSPPAVINAVLDALKPLGVTDIDMPATPHRVWQAIQNAQA"
     gene            complement(4038..4505)
                     /locus_tag="PK518_10320"
     CDS             complement(4038..4505)
                     /locus_tag="PK518_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRM49352.1"
                     /translation="MQVELKVNGKPVSADVPPNTLLVQLLREHLRLTGTHVGCDTAQC
                     GACTVMADGRAIKSCNVLALQMQGADITTVEGLAQADGTLHPVQAAFKECHGLQCGFC
                     TPGMVMSTVDLLAHHPQASEAEIRHLLEGNLCRCTGYQNIVKAVQTAQKAMAA"
     gene            complement(4669..5787)
                     /locus_tag="PK518_10325"
     CDS             complement(4669..5787)
                     /locus_tag="PK518_10325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM49353.1"
                     /translation="MNTQTQAGALAGIKVLDLSRILGGPLCGQILGDHGADVLKVEPP
                     QGDDTRAWGPPFKDGVASYYFGLNRNKRIQFLDLSAAEGQQRVRELMGEADVVVENFK
                     VGTMEKWGIGYEQVREQFPRLVWCRVTGFGADGPLGALPGYDAAVQAMAGLMSINGDA
                     DGDPLRVGLPVVDMVTGMNAVIGVLLALHERGRSGQGQLVDAALYDSGLSLLHPHAPN
                     WFMSGKIPQRTGNAHPNIYPYDVIHTGGAPIFLAVGNDRQFRLLCDYIGQPAMADDAR
                     FATAGQRSVNRAVLKPLLEQAFTQRDGQELADALMAIGVPAAPVLNVEQALHHPHTVH
                     REMVVKMGDYQGLGAPVKLSRTPASYRFAPLAEGKDFM"
     gene            complement(5881..6858)
                     /locus_tag="PK518_10330"
     CDS             complement(5881..6858)
                     /locus_tag="PK518_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49354.1"
                     /translation="MQKRKLLTLIAGAAMLAALPVQAQGSFPDKLITFVVPFPPGGPT
                     DAMARILATELTKELGQSVIVDNRAGAGGNIGADAVARATPDGYTIMFGTSGPLAINQ
                     SLYKGQKYDARTSFEPIVYVGYLPNILVVRSGLGVANVQELIAKEKAKPGTLNYASSG
                     NGASSHLAGVLFNEMTGTKLLHVPYKGTGPALNDLLGGQVDMTFTDILTAMPYIKTDK
                     VKALGVATAKRSSAMPDIPTIAEQGVKGYDVSVFFGVVAPKGTPADRIKVLNQAFSRA
                     LDSDRVKQSFAQQGLEGSSDRSPAYLAKFIKSEVDKWAKVVKQAGVQLD"
     gene            complement(7075..8781)
                     /locus_tag="PK518_10335"
     CDS             complement(7075..8781)
                     /locus_tag="PK518_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM49355.1"
                     /translation="MSATDQTQMTALAQASSAAHPVPDRGHVNNYREDTELQQLLALY
                     LPADLYAHLQPYFDRMGELAGGAVDDWALEADRNPPTLEMRNRAGQEVQRIVKHPAYI
                     EMEKVAYQQFGLAAISHREDMLGWKGKMPAIVKYALTYLFVQSEFGLCCPLSMTDSLT
                     RTLRKYGDEKLVAKYLPKLLAMDFDSAVQGSMFMTEQAAGSDISNTLTMAYPQEDGSW
                     RLYGEKWFCSNPDAGFGMVLARVEGGPPGMKGISLFLLPRQLDDGSTNHYTIVRLKDK
                     MGTRSMASGEIRMEGAVAYLVGEQGRGFVQIADMVNNSRLSNGMRSAGMMRRAVAEAE
                     YIARERWAFGKQLQDMPLMKVQLDKLRVPAEQARTMVFQTAATLTRSDAGDPEAYALL
                     RILTPMIKFRACRDARKVTGDAMEVRGGCGYIEEWADPRLVRDAHLGSIWEGTSNIVA
                     LDVKRAIKREGSLPVLQAYLHGLLKDTQGITAAYRQALTDALAGAVQLAERAVADDGE
                     YLVRQAATALYNCTTAIAMAWEACKTGSQERLRLSQLVIAHRVLPRDPLNAGGVPAEW
                     LQ"
     gene            8897..9784
                     /locus_tag="PK518_10340"
     CDS             8897..9784
                     /locus_tag="PK518_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49356.1"
                     /translation="MELRVLHYFVTLAEELHFGRAAQRLRISQPPLSVAIKQLEEELQ
                     AQLFERNSKGVRLTAAGEHLLAKARQLLALSHQAAQETRDVARGTVGHLRLGFVGSSL
                     YRGLPQALEAMQQTHPQVRVDMLEANSAEQILGLQQMRLDLALVHSIQPPEGIASQLI
                     VDEPFVVCLPEQHPLCAAAAIDLGALKGDRLILFSTLVSPTYHQRIYEMCLAHGFAPE
                     VRHEVRHWLSVISLVSLGQGVALVPAALERVGLPRLQFRPLLGEHPHSEMLALWRTAP
                     ASPLVQALLAHLRQAAQGC"
     gene            10076..>11414
                     /locus_tag="PK518_10345"
     CDS             10076..>11414
                     /locus_tag="PK518_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRM49357.1"
                     /translation="MPLSWNEIKHKALAFSKHWADAANEDSEAKPFWIAFFEIFGITD
                     KRVATFELNVKKHGGGQGYVDLFWPGMLLVEQKSRGRSLDAAFSQALGYFPGIAERDL
                     PQIIIVCDFARFRVHHLATGRTTEFALQDLHKHVRLLGFIAGYRAQDIQAQDPVNIRA
                     AERMGKLHDALKASGYTDHPLEVLLVRLLFCMFADDTGIFQPAQALRSFVEERTEEDG
                     SDLGPRLAQLFQVLNTPEDRRSKNLDEQLAAFPYVNGRLFEESLPMADFDAAMRGALL
                     DACALDWSAISPAIFGSLFQSIMDDKARRNLGAHYTSEENILKLIKPLFLDALWAEFE
                     KVKNHKNRLFEFHKKLRQLTFFDPACGCGNFLVISYRELRLLELEVLRASYRDGQQTL
                     DVHQLISLNVDQFFGIEIEEFPAQIAQVALWLVDHQMNLRVSEEFGLYFARIPL"
BASE COUNT         1825 a         3807 c         3766 g         2016 t
ORIGIN      
        1 tcgcgctcgg ggtagtccag ccagtggtac aggcagcggc gcttgacggc gtcgttcagc
       61 tcacgcgtgc ggttgctggt caggatggtc accggcggca cggcggcgct gatggtgccc
      121 agctcgggaa tgctgacctg gtactcgccc aggtattcga gcagaaaggc ctcgaagggc
      181 tcgtcggcac gatccacctc gtcgatcagg agcagcgcgc ccggcgccgg ggcttcgagc
      241 gcctgcagga gcgggcggcg caccaggtag cgcggctggt agacctcctg ctccagtgcg
      301 ttggccgacg ccgtaccctc ggccgcacgc aggtgcagca gctgggcggc gtagttccat
      361 tcatacaggg cctcccgctg ctccaggccg tcaaagcatt gcaggcgcag cagctggcgc
      421 gagagcacct gggccagcgc cttggccagc tcggtcttgc ccacgccggg ctcgccctcc
      481 agcagcagcg ggcgctgcag cttcagcgcc aggaacaccg ccgtggccag gcgccggtcg
      541 gcaaagtagc cgacggactg cagggcggcg gccagttcgt cgatgctggt gcaggcacag
      601 ggtggttgtg gcatgagcgg agtattcaaa aacaatagct gtttgcgctt gctgagtatg
      661 ggatagatgc caaaaatatc tgaaccatgg catgtcccga attccgcccc ctctggggga
      721 aggcggggat gggggccggg agcgccggca tcttgccggc atgaagacgc aggccttgct
      781 gccccccacc cctaccctcg cccggtgggg gagggagtgg cgcggctcaa gccagcgcct
      841 gggccacggc gcgctgcgcc atcacgccca ccaggtgggc gcggtaagcc gcgctgccat
      901 gcaggtcgct ggacatctca ccctcgtccg tggccacgcc ggcgacggca tcgggcgtga
      961 agctggccga cagcgccgcc tccaggcccg catggcggaa cacgccgttg ccgccgccgg
     1021 tcacggcgac ccgggcgcca ccatcggtct gcgcgacaaa cacgccaatc agcgcgaaca
     1081 gcgaggccgg ctgcacgaac ttgacgtagg ccgccttctt cgggatggga aagctcaccg
     1141 cggtgatcag ctcgccctct tccagcgccg tggcaaacag cccctggaag aagtcatcgg
     1201 ccgcgatggc gcgcgccgtg gtgtgcaccg tggcgcccag cgccagcagc gcggcggggt
     1261 agtcggcggc cgggtcgttg ttggccaccg aaccgccaag cgttccccgc gcgcgcacct
     1321 gcttgtcgcc aatgccgccg gccaatgctg ccagcgcggg gatggcggcg cgcagctcgg
     1381 cgctttcagc cacgtcggcg tggcaggtca tggcgccgat gacgatgctg ttgccctcgc
     1441 ggcgtatgcc cttgagctcg gggatggcgc ccaggtccac cagctgctcg ggcgcggcca
     1501 ggcgcagctt catggatgcc agcagggtct ggccgccggc cagcagcttg ccgccggcct
     1561 gggccagctt caaggcgtcg gcctgggagg cggggcgttc gtaggtgaat gcgtacatgg
     1621 tgcttgtcct ttcaagcctg ggcgttctgg atggcctgcc agacgcggtg cggcgtggcg
     1681 ggcatgtcga tgtcggtcac gcccaggggt ttgagcgcat ccagcacggc gttgatgacg
     1741 gccggcggcg agcctatggc ccccgcctcg ccgcagccct tgctgccgat ggggttgtgc
     1801 gtgcagggcg tgcacacatg accgagtttg aactcaggga agttggcggc gcgcggcatg
     1861 gcgtagtcca tgtagctgcc ggtgagcagc tggccgctgt ccgggtcata cacgccatgc
     1921 tccaagagtg cctggcctat gccctgcacc aggccgccat gcacctggcc ctcgacgatc
     1981 atggggttga tgatgacgcc gaagtcgtcc accgcggtga agcggtccac gcgcaccacg
     2041 ccggtctgcg ggtctacctc cacctcgcag atataggtgc ctgcgggaaa ggtgaagttc
     2101 accgggtcgt agaaggcggt ctcgttcagg ccgggctcca gcttgtccag cgggtagttg
     2161 tgcggcacat aggccgtcag cgccacctgg gcaaagggga tcttcttgtc cgtgcccttc
     2221 accgtgaact cgccgccggc aaactcgatg tcggcgtcgc tcgcctccat caaatgcgcg
     2281 gcgattttct tggccttggc ctcgatcttg tccagcgcct tcatgatggc cgtgccaccc
     2341 acgctcaggg accggctgcc gtaggtgccc atgccaaagg gcacgcggcc ggtgtcgccg
     2401 tgcacgatgt ccacctggtc cggcgacagc cccaggcgcg ccgccaccag ctgggcaaac
     2461 gtggtctcat gcccctggcc atggctatgc gagccggtga acaccgtcac gctgcccgtg
     2521 gggtgcacgc gcacctcgcc ggcctcgaac agcccggccc tggcccccag ggcgccggcg
     2581 atgttgctgg gcgccaggcc gcaggcctcg atatagctgc tgtagcccat gccgcgcttg
     2641 aggcccctgg cctcgctggc cttgcggcgc gcgtcgaagc ccgccacgtc ggccagctgc
     2701 tgcgcctggt tcatgcaggc gtggtagtcg ccaatgtcgt actgcagcgc caccggcgtc
     2761 tgatagggga agctggtgat gaagttgcgc ttgcggattt cgtcctggga caggcccaga
     2821 tcccaggcgc agcgcgtgac caggcgctcg accacatagg tggcctcggg ccggccggcg
     2881 ccgcggtagg catcgaccgg cgcggtgctg gtgaaccagc catccacctc cacatagatc
     2941 tgcggcgcgg tgtactggcc ggccagcagc gtgccataca ggatggtggg cacgctggag
     3001 gcaaacgttg acaggtaggc gcccaggttg gcgtcggtgt gcacgcgcat ggcgaggaac
     3061 ttgccttgac tgtccatggc catctcggca tgggtgatgt ggtcgcggcc atgggcgtcg
     3121 gacacgaacg actcgccgcg ctcggccgtc cacttcacgg cgcagttgag ctgcttggcg
     3181 gcccaggtga cggccacgtc ctcggcatac agaaagatct tcgagccgaa accgccgccc
     3241 acgtcgggcg cgatgacgcg caccttgtgc tcgggcagct gcagcacgaa ggcggtcagc
     3301 agcaggcgct ccacatgcgg gttctggttg gccacgtaga gcacatattc gtcggtggcg
     3361 cggttgtagc tggcgttggc ggcgcgcggc tctatggcgt tggggatcag gcggttgttc
     3421 accagatcca gtttgctgac atgcgcggcg ccggcaaagg cggcgtccac cgccgccttg
     3481 tcgcccagcg tccatttgta gcaatggttg tcgggggcct cgtcgtgcag cgccgggccg
     3541 tccttggcct ttttcatgtc gatgaccggg gccagctcct catagtccac ctgcacggcc
     3601 tcggcggcgt tcctggcctg ctccagcgtg tcggccacca ccatggccac atggtcgccc
     3661 acgtagcgca ccttgcctat ggccaggatg gggtgcatgg gctccttcat ggggctgccg
     3721 tcggggttgc tgatcaacca gccgcagggc aggccgccca tttttccttc aatgtccttg
     3781 ccgctgtaga tggcggccac gccgggcatc ttggcggcct cggccgtgtc cacgcccttg
     3841 acgcgcgcat gtgcgtgcgg cgagcgcacg aagtacgcgt acttctggtt cgcctggtca
     3901 acatcgtcgg tgtagttgcc cacgccggtc agaaagcgca cgtcctcctt gcgcttgagc
     3961 gattcgccga tatgcggcag cttggcaaat gcattggcac ccatggtctt gtctcctggt
     4021 ggtggttttc aggggcctca ggcggccatg gccttctggg cggtctgcac cgccttgacg
     4081 atgttctggt agccggtgca gcggcacagg ttgccctcca gcaggtggcg tatctcggcc
     4141 tcgctggcct gggggtggtg ggccagcaga tcgacggtgc tcatcaccat gccgggggta
     4201 cagaagccgc attgcaggcc atggcactcc ttgaacgccg cctgcaccgg gtgcaacgtg
     4261 ccgtcggcct gggccaggcc ttcgacggtg gtgatgtccg cgccctgcat ctgcagcgcc
     4321 agcacgttgc acgacttgat ggcccggcca tcggccatca ctgtgcaggc gccgcattgc
     4381 gcggtgtcgc agcccacatg ggtgccagtc aggcgcagat gctcgcgcag caattgcacc
     4441 agcagcgtgt tgggcggcac atccgccgaa accggcttgc cattgacctt gagctctact
     4501 tgcatggtgg gcgtctcctt gtgtggcgtg gttgtcttgg ttgtaaaaaa gccgttctat
     4561 tcttggaaca acccacgcca acacacctgg gtaaaaccct tgcccgtgtc acatgcgtaa
     4621 aaaaaagggc ccgcacatgc gggcccggca atagggtgca accgtcggtt acatgaaatc
     4681 cttgccctcg gccagcggcg caaagcggta gctggccggc gtgcggctga gcttcaccgg
     4741 cgcgcccagg ccctggtagt cgcccatctt caccaccatc tcgcggtgca cggtgtgcgg
     4801 gtgatgcagg gcctgctcca cgttcagcac cggcgcggcg ggcacgccta tggccatcag
     4861 ggcatcggcc agctcctggc cgtcgcgctg ggtgaaggcc tgctccagca ggggtttcag
     4921 caccgcgcgg ttcaccgagc gctggccggc cgtggcaaag cgtgcgtcgt cggccatggc
     4981 cggctggccg atatagtcgc acagcaggcg gaactgccgg tcgttgccca cggccaggaa
     5041 gatcggtgcg ccgccagtgt ggatcacgtc gtaggggtag atgttggggt gggcgttgcc
     5101 cgtgcgctgc gggatcttgc cgctcatgaa ccagttgggc gcgtgcgggt gcagcagcga
     5161 caggccgctg tcgtacagcg ccgcatccac cagctggccc tggccgcttc tgccgcgctc
     5221 gtgcagcgcc agcagcacgc cgatgacggc gttcatgccg gtgaccatgt ccaccaccgg
     5281 caggcccacg cgcagcgggt cgccgtcggc gtcgccgttg atgctcatca gcccggccat
     5341 ggcctgcacc gccgcgtcgt agccgggcag cgcgcccagc gggccgtcgg cgccaaagcc
     5401 ggtcacgcgg caccagacca ggcgcggaaa ctgctcgcgc acctgctcgt agcctatgcc
     5461 ccatttctcc atggtgccga ccttgaagtt ctccaccacc acgtcggcct cgcccatcag
     5521 ctcgcgcacg cgctgctggc cctctgcggc cgacagatcc aggaactgga tgcgcttgtt
     5581 gcggttcagc ccgaagtagt aggaagccac gccgtcctta aagggagggc cccaggcgcg
     5641 cgtgtcgtcg ccctggggtg gctccacctt gagcacgtcg gcaccatggt cgcccaggat
     5701 ctggccgcac agcgggccac ccaggatgcg cgacaggtcg agaaccttga tgccggcgag
     5761 tgcgccggcc tgggtttgcg tattcattgc tttttctttc gacacatgag ggggtgtagg
     5821 agcggcttta gccgcgaaca tgttcattcg cggctaaagc cgctcctacg gggaatgtgg
     5881 tcaatccagt tgcacgccgg cctgcttgac caccttggcc catttgtcca cctcggactt
     5941 gatgaacttt gccagatagg ccgggctgcg gtcggacgag ccctccagcc cctgctgggc
     6001 aaagctctgc ttgaccctgt cggagtcgag cgcgcgcgag aacgcctggt tcaagacctt
     6061 gatacggtcg gccggcgtgc ccttgggggc caccacgcca aagaacacgc tcacgtcgta
     6121 gcccttcacg ccctgctcgg cgatcgtcgg gatgtccggc atggcgctgg agcgcttggc
     6181 cgtggccacg cccagggcct tgaccttgtc ggtcttgata tagggcatgg cggtgaggat
     6241 gtcggtaaac gtcatgtcca cctgcccgcc cagcagatcg ttgagcgccg ggcccgtgcc
     6301 cttgtagggc acatgcagca gcttggtgcc ggtcatctcg ttgaacagca cgcccgccag
     6361 gtgcgacgag gcgccgttgc ccgacgatgc gtagttcagc gtgccgggct tggccttttc
     6421 cttggcgatc agctcctgca cattggccac gcccaggccg gagcgcacca ccaggatgtt
     6481 gggcaggtag cccacgtaga cgatgggttc gaagctggtg cgcgcgtcgt acttctgccc
     6541 cttgtacagg ctctggttga tggccagcgg cccagatgtg ccaaacatga tggtgtagcc
     6601 atcgggcgtg gcgcgcgcca cggcatcggc gccgatgttg ccgcccgcgc ccgccctgtt
     6661 gtccacgatc acgctctggc ccagctcctt ggtcagttcg gtggccagga tgcgcgccat
     6721 ggcatccgtg ggcccgcccg gcgggaaggg cacgacgaag gtgatcagct tgtcggggaa
     6781 gctgccctgc gcctgaaccg gcaaggcggc cagcatggcg gcgccggcga tgagggtgag
     6841 tagcttgcgt ttttgcatgg tttgtctcct gttgttgatt tgatagctgg tggcgcttga
     6901 tacataagcg ctagaggctg tttctccctc tcccgctggc gggagagggc tggggtgagg
     6961 ggcgtaaaag tgggcactgc gcccaatcac cccctcaccc ctaccctctc ccccaagggg
     7021 gcgagggaat taaaacctgc cctctcccag aggggggagg gagttatcgc gtgcttactg
     7081 cagccactcg gccggcacgc cgcctgcatt caaggggtcg cgcggcagca cgcggtgggc
     7141 tatcaccagc tgagacaggc gcaggcgctc ttgcgagccg gtcttgcacg cctcccaggc
     7201 catggcaatc gccgtggtgc agttgtacag cgcggtggcg gcctggcgca ccaggtattc
     7261 gccatcgtcg gccacggcgc gttcggccag ctgcacggcg ccggccagcg catcggtgag
     7321 cgcctggcgg taggcggcgg tgatgccctg ggtgtccttg agcagaccat gcagataggc
     7381 ctgcagcacc ggcagcgagc cttcgcgctt gatggcgcgc ttgacgtcca gcgccacgat
     7441 gttgctggtg ccctcccaga tcgagcccag gtgggcgtcg cgcaccaggc gcgggtcggc
     7501 ccattcctcg atgtagccgc agccgccgcg cacctccatg gcgtcgcccg tcaccttgcg
     7561 tgcgtcgcgg caggcgcgga acttgatcat gggcgtcagg atgcgcaaga gcgcataggc
     7621 ttcagggtcg ccagcgtccg agcgcgtcag cgtagccgcg gtctggaaca ccatggtgcg
     7681 cgcctgctcg gccggcacgc gcagcttgtc cagctgcacc ttcatcagcg gcatgtcctg
     7741 cagttgcttg ccaaaggccc agcgctcgcg ggcgatgtac tcggcctcgg ccacggcgcg
     7801 gcgcatcatg ccagcggagc gcatgccgtt cgagaggcgc gagttgttca ccatgtcggc
     7861 aatctgcacg aagccccggc cctgctcgcc caccaggtag gccacggcgc cttccatccg
     7921 gatctcgccg ctggccatgg agcgcgtgcc catcttgtcc ttcaggcgca cgatggtgta
     7981 gtggttggtg ctgccatcgt caagctgacg cggcagcagg aacagcgaga tgcccttcat
     8041 gccgggcggc ccgccctcca cgcgcgccaa caccatgcca aagcccgcgt cggggttgga
     8101 gcagaaccac ttctcgccat acaggcgcca gctgccgtcc tcctgcggat aggccatggt
     8161 cagggtgttg gagatgtccg agccggccgc ctgctcggtc atgaacatcg agccctgcac
     8221 ggccgaatcg aagtccatgg ccagcagctt gggcaggtat ttggcgacca gcttttcgtc
     8281 gccatacttg cgcagcgtgc gcgtcagcga atcggtcatc gacagcgggc agcacaggcc
     8341 gaactcggac tgcacgaaca agtacgtcag cgcgtacttg acgatggccg gcatcttgcc
     8401 cttccagccc agcatgtcct cgcggtgcga aatggcggcc aggccaaact gctggtaggc
     8461 caccttctcc atctcgatgt aggcggggtg cttgacgatg cgctgcacct cctggccagc
     8521 gcggttgcgc atctccagcg tcggggggtt gcggtcggcc tccagcgccc agtcatcgac
     8581 ggcgccgccg gcgagctcgc ccatgcggtc aaagtagggc tgcaggtggg catacaggtc
     8641 ggccggcaga tacagcgcca gcagctgctg cagctcggtg tcttcgcgat agttgttcac
     8701 atggccgcga tccggcacgg gatgggcggc agagctggcc tgggccaggg cggtcatctg
     8761 ggtctgatcg gtagcggaca tggctggtct cctttgaatc tcggctgaca tcgggcttgg
     8821 ggctattgca ccagttttaa tcattcgagt ccaatatcat taaaatcttt gtcaatattt
     8881 aatttgtata aatgccatgg aactgcgtgt actgcactac ttcgtgaccc tggccgagga
     8941 actgcacttc ggccgcgcgg cgcagcggct gcgcatctcg cagccaccgc tgtcggtcgc
     9001 catcaagcag ctggaggaag agctgcaggc ccagctgttc gagcgcaaca gcaagggcgt
     9061 gcgcctgacg gccgccggcg agcatctgct ggccaaggcg cgccagctgc tcgccctgag
     9121 ccaccaggcg gcgcaagaga cgcgcgacgt ggcgcgcggc accgtcggcc acctgcgcct
     9181 gggctttgtc ggatccagcc tgtaccgcgg cctgccccag gcgctggagg ccatgcagca
     9241 gacccacccc caggtgcgcg tggacatgct ggaggccaac agcgccgagc agatcctggg
     9301 cctgcagcaa atgcgcctgg acctggccct ggtgcactcc atacagccgc ccgagggcat
     9361 cgccagccag ctcatcgtgg acgagccctt cgtcgtctgc ctgcccgagc agcacccgct
     9421 gtgcgccgcc gcagccatag acctgggcgc gctgaaaggc gaccggctga ttttgttctc
     9481 caccctggta tcgcccacct accaccagcg catctacgaa atgtgcctgg cccacggctt
     9541 cgcccccgag gtgcgccacg aggtgcgcca ctggctgtcg gtgatctcgc tcgtctcgct
     9601 gggccagggc gtggccctgg tgcccgcggc gctggagcgc gtgggcctgc cgcgcctgca
     9661 gtttcgcccc ttgctgggcg agcacccgca ttccgaaatg ctggccctgt ggcgcaccgc
     9721 gccggccagc ccactggtac aggcgctgct ggcgcatttg cgccaggcgg cgcagggctg
     9781 ttgacggatc gcctgtagac tattaaaaaa atagctgctg gcgcttgata cgcaagtgct
     9841 acaaccttgt ttcacctgat ttcacacatg cctcagaccc ggccgcaggc cctcaccccc
     9901 caccctctcc cagagggaga gggagaggga gtaaaaccag cgcaaccggt cacccccacc
     9961 cctgttgggc gagggggtca agccccggca accgatcgcc ctcgcccctt tggggagagg
    10021 gaggggtgag gggcttgcac ggcaagcacc tggcccacca acctccactt gacccatgcc
    10081 cctgtcctgg aacgaaatca agcacaaggc cctggccttc tccaagcact gggcggacgc
    10141 cgccaacgaa gactccgagg ccaagccctt ctggatcgcc ttcttcgaga tcttcggcat
    10201 caccgacaag cgcgtcgcca ccttcgagct caacgtcaag aagcacggtg gcggccaggg
    10261 atatgtggac ctgttctggc ccggcatgct gctggtcgag caaaagtcgc gtgggcgcag
    10321 cctggacgcg gcctttagcc aggcgctggg ctacttcccc ggcatcgcag agcgcgacct
    10381 gccgcaaatc atcatcgtct gcgacttcgc gcgctttcgc gtgcaccacc tggccacggg
    10441 caggacgacc gaatttgcac tgcaagacct gcacaagcat gtccgcctgc tcggcttcat
    10501 cgccggctac cgggcccagg acatccaggc gcaagacccc gtcaacatcc gcgccgccga
    10561 gcgcatgggc aagctccacg acgcgctgaa agccagcggc tacaccgatc acccgctgga
    10621 ggtgctgctg gtgcgcctgc tgttttgcat gtttgccgac gacaccggca tcttccagcc
    10681 cgcccaggcg ctgcgcagct ttgtcgagga acgcaccgag gaagacggca gcgacctggg
    10741 cccgcgcctg gcgcagctct tccaggtgct gaacacgccc gaggacaggc gcagcaaaaa
    10801 cctggacgag caactcgccg ccttccccta cgtcaacggc cggctgtttg aagaaagcct
    10861 gcccatggcc gacttcgacg ccgccatgcg cggcgcgctg ctcgacgcct gcgcgctcga
    10921 ctggtcggcc atcagtcccg ccatcttcgg cagcctgttc caaagcatca tggacgacaa
    10981 ggcgcggcgc aacctgggcg cgcattacac gagcgaggaa aacatcctca agctcatcaa
    11041 gccgctattt ctggacgcgc tgtgggccga gttcgagaag gtcaaaaacc acaagaaccg
    11101 gctctttgaa ttccacaaaa aactacgcca gctgacgttt ttcgaccccg cctgcggctg
    11161 cggcaacttc ctcgtcatca gctaccgcga gctgcgcctg ctcgaattgg aagtgctgcg
    11221 cgccagctac cgtgacggcc agcagaccct ggacgtgcac cagctcatca gcctgaacgt
    11281 cgatcagttc tttggcatag aaatcgaaga attcccggcg cagatcgccc aggtcgccct
    11341 gtggctggtg gaccaccaga tgaacctgcg cgtgagcgaa gagtttggcc tgtacttcgc
    11401 gcgcatcccg ctca
//
LOCUS       DAOMTQ010000100        11361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1063_length_11361_cov_27.369538, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000100 DAOMTQ010000000
VERSION     DAOMTQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1063_length_11361_cov_27.369538"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK518_10350"
     CDS             complement(<1..508)
                     /locus_tag="PK518_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM49358.1"
                     /translation="MRSIADSLHHQAAEKLRQQIFAGELAPGSFVDEAALCEQLAVSR
                     TPLREALKVLVAEGLLRHEPRRGCFVAEVTERDLDEIFPVIALLEGRAAHEATQKASS
                     ADVAALETLHQRLQQCASEGRIPDYYAANHAIHEAFITLADNRWLAQVIGDLRKILRL
                     ARHQTLHVP"
     gene            802..1677
                     /locus_tag="PK518_10355"
     CDS             802..1677
                     /locus_tag="PK518_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRM49359.1"
                     /translation="MTQNTHKTVLSTRSAPERHWVGDGFPVHGLFGYSGAGVAERSPF
                     LLLDYAAPTTFTPTTARRGVGSHPHRGFETVTIVYDGEVEHRDSTGAGGIIGAGDVQW
                     MTAGGGIIHEEFHSSAYAASGGPFEMVQLWVNLPASQKMTPARYQAILDAQIPAVALP
                     GGAGRVRVIAGSFDGRQGPAHSFTPMNVLDMRLAAGHRLQLAQPEGWSTLVVVLAGTV
                     QINDGTTLGAAEMASLSTQGTQLTLAAQEDAKLLLLAGEPIAEPVVGYGPFVMNSEQQ
                     IAQAIDDFNRGHFTH"
     gene            1692..2606
                     /locus_tag="PK518_10360"
     CDS             1692..2606
                     /locus_tag="PK518_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRM49360.1"
                     /translation="MTDSQRETPPQRYGARATVIGEDTRILRALPHRERRTVGAWCFL
                     DHLGPVQFAPGKGMHVGAHPHIGLQTFTWMIEGEVVHRDSLGNEQTIRPGQVNLMTAG
                     RGIAHTEDSLLEGARLHAAQLWIALPDAQRHCAPAFENYPELPQVDAGGWRATVLAGS
                     ALGQTAPAQVYTPLVGLDMASTGPAQTQLPLQPGFEYAAMVLRGAATVAGEPLAPGEW
                     LYFAPGRTTLDARCDAAAQWLLLGGEPLGEDILIWWNLVARNQAELQSALDDWNAGRF
                     APVRPGSPAEPLPAPSLSGARVRASGQA"
     gene            2955..3728
                     /locus_tag="PK518_10365"
     CDS             2955..3728
                     /locus_tag="PK518_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM49361.1"
                     /translation="MSVKQLFDLTGQVALVTGGSRGLGLQMAQALGEMGARLAITARK
                     ADELAQARAQLEGLGYEVETIVNDLSKFEQIPAMVDQVLARFGTIDILVNNAGATWGA
                     KAEDYPDAAWNKVMDLNVNAPFFLSREVGRRVMIPKGRGNIIITASVAALKGTPPGMN
                     TIAYNTSKAAALHFARTLASEWGPYGIRVNAICPGFFPSKLASGLIDKLGPALIERTP
                     LRRIGGDEDLMGAVVFLASNASRHITGQGLVVDGGASLI"
     gene            3750..4982
                     /locus_tag="PK518_10370"
     CDS             3750..4982
                     /locus_tag="PK518_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM49362.1"
                     /translation="MDFSYSPKVVDLQQRLTAFMDEHIYPAEAVYHAEVEANRRAGNA
                     WQPTRVMEDLKAKARAAGLWNLFLPESQYGAGLTNLEYAPLCEIMGRSHVAPEAFNCS
                     APDTGNMETLVRYATPEQQQRWLLPLLDGKIRSAFAMTEPAVASSDATNIEARIERVG
                     DEYVINGRKWWTSGAPDPRCEILIFMGKTDPGHASRHRQQSMILVPMKTPGVTMERAL
                     SVFGYDHAPHGHGEVSFDNVRVPVANVLLGEGRGFEIAQGRLGPGRIHHCMRLIGVAE
                     RALELMCRRALGRVAFHRPVADQGVTRERIANARILIDQARFLVLNAAYMMDTVGNKV
                     AQKEIAMIKVAAPSMACQVIDWAMQVHGGAGVSDDFPLAAAYAHARTLRFADGPDEVH
                     RNQIGKMELARYMALDKR"
     gene            4991..6688
                     /locus_tag="PK518_10375"
     CDS             4991..6688
                     /locus_tag="PK518_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRM49363.1"
                     /translation="MSKRHLAHWPRLLPQHLTLPATHLYRNLEVSAMRYPDKAALVFY
                     DAAISYARLQDESERLAAHLQQRCGVRRGDRVLLYLQNSPQFVIGYYAILRADAVVVP
                     VNPMNLSEELRHYVLDAGARTILAPQDLWAQVRPLLHEGLAAAAADAQAPGLRHAVVA
                     AYGDYLTAPTDLAVPAFVSAPREPVEEPGACAWMDALAAGLRPGPIAAGPDDLAVMPY
                     TSGTTGQPKGCMHTHRSVMYNTVSGGIWLGTTPDAVSLTVLPLFHVTGMQGGMNSPLY
                     TGSTIVLLPRWDRDAAAQLIARYRVTNTQMIVTMVVDLLSNPRLAEYDLSSIRRLSGG
                     GAAMPEAVAAKLQELCQIDYIEGYGMSETMAATHINPPDRPRKQCLGIPIFDVDARVV
                     DPATFTELPPGEVGEIIVHGPQLMQGYWGQAEATAQAFVTLDGKRFLRTGDLARTDDD
                     GYFYMVDRLKRMINASGYKVWPAEVEALMYAHPAIQEVCVIAAQDARRGETVKAVVVL
                     REAFRGQVSEQDIVDWAHGHMAAYKSPRLVSFVDSLPKSGTGKVQWRALQERESAPR"
     gene            6779..>7290
                     /locus_tag="PK518_10380"
     CDS             6779..>7290
                     /locus_tag="PK518_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HRM49364.1"
                     /translation="MDRFQFCASLVCGAALAALPRAAFAQATPAQRLDTPRAIDMAGR
                     LRMLSQRCTKAYLQWGQSIAGDSARTLLQESVSRYEAHLAALKTFMPNPAVQASLPQL
                     QTLWSQFKALLTGEPSRTGAAALYDANETLQSAAHYLTVSYGNTNGTPLEYLIGLAGR
                     QRMLSQRMAK"
     assembly_gap    7291..7390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7391..8008)
                     /gene="lgt"
                     /locus_tag="PK518_10385"
     CDS             complement(<7391..8008)
                     /gene="lgt"
                     /locus_tag="PK518_10385"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRM49365.1"
                     /translation="MLMYPQIDPVALQIGPVAIHWYGLTYLAAFGLFMLLGLRRLRHE
                     PYASLTGEQAWSRKDVEDILFLGVLGVVIGGRLGYCLFYKPGYYLTHPLEIFYVWQGG
                     MSFHGGLLGVIGSMLWFARSRHRPWLQVADFVAPCVPTGLAAGRIGNFINGELWGRMA
                     SPDLPWAMVFPQSGSMLPRHPSQIYQFLLEGLLLFVLLWLYARRPR"
     gene            8107..8577
                     /gene="bfr"
                     /locus_tag="PK518_10390"
     CDS             8107..8577
                     /gene="bfr"
                     /locus_tag="PK518_10390"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRM49366.1"
                     /translation="MQGHTAVIDCLKELLRGELAARDQYFIHSRQYEDQGFVRLYERL
                     NHEMQEETEHADAILRRILMLEGKPDMRPHAFTPGESVQEMLRKDLDTEYSVRKHLKS
                     AIALCESHHDYVSRDLLLTQLRDTEEDHAYWLEKQIGLIDKVGLQNYLQSQTGS"
     gene            complement(8578..9006)
                     /locus_tag="PK518_10395"
     CDS             complement(8578..9006)
                     /locus_tag="PK518_10395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRM49367.1"
                     /translation="MSELHQRIAASFNAQGLMTTLGARLVLVQDGEVHIELPFSPHLT
                     QQQGFVHAGVVTSIVDNACGYAALTRSPPGCEIVTAEFKTNFMRPAIGARFLAVGRVQ
                     NAGRMLTVCTGEVRAFAGDGPQYKLVALMQATMVNVPANA"
     gene            complement(9012..10871)
                     /gene="ilvD"
                     /locus_tag="PK518_10400"
     CDS             complement(9012..10871)
                     /gene="ilvD"
                     /locus_tag="PK518_10400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM49368.1"
                     /translation="MPAYRSKTSTHGRNMAGARALWRATGMKDADFSKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDIIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVIFVSGGPMEAGKAQLAVPGQ
                     GKLTFTKKLDLIDAMVMAADDKASDADVAEVERSACPTCGSCSGMFTANSMNCLTEAL
                     GLSLPGNGTVLATHADREQLFKRAGRRIVELAREYYEQDNQRILPRAVGFKAFENAMT
                     LDIAMGGSTNTILHLLAIAQEAEIAFTMDDIDRLSRVVPQLCKVAPNTDKYHIEDVHR
                     AGGIFGILGELERAGRLHTDVPTIHAPTLGEAIAQWDICLTQDEAVHNFFKAGPGGIP
                     TQVAFSQASRWPSLDLDRAEGCIRSYDHAFSKEGGLAVLTGNIAQKGCVVKTAGVDDS
                     ILVFEGPAHVVESQDEAVANILADKVKAGDVVVVRYEGPKGGPGMQEMLYPTSYIKSK
                     GLGKACALLTDGRFSGGTSGLSIGHCSPEAAAGGAIGLVRNGDRIRIDIPNRTINVLV
                     SDEELARRRQQQAALGWKPAQPRPRKVSAALKAYAKLVTSADTGAVRDLSLLD"
     gene            11064..>11361
                     /locus_tag="PK518_10405"
     CDS             11064..>11361
                     /locus_tag="PK518_10405"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM49369.1"
                     /translation="MTDPLPLNRRSAHITQGKARAANRSMYYGMGYTEADFNKPMVGV
                     ANGHSTITPCNSGLQRLADAAVDGIRAAGGNAQTFGTPTISDGMAMGTEGMKYSL"
BASE COUNT         1909 a         3907 c         3729 g         1716 t
ORIGIN      
        1 ccggcacatg cagcgtctgg tggcgcgcca ggcgcaggat cttgcgcagg tcgcctatca
       61 cctgggccag ccagcggttg tcggccaggg tgatgaaggc ctcgtggatg gcgtggttgg
      121 cggcgtagta gtcggggatg cggccttcgc tggcgcattg ctgcaggcgc tggtgcaggg
      181 tttcgagcgc tgccacatcg gcactgctgg ccttttgtgt ggcctcgtgg gcggcgcggc
      241 cttccagcaa ggcgatcacg gggaagatct cgtccaggtc gcgctccgtc acctcggcca
      301 caaagcagcc ccggcgcggc tcgtggcgca gcagcccctc ggccaccagc accttcagcg
      361 cctcgcgcag cggcgtgcgc gaaacggcca gctgctcgca cagcgccgcc tcatcgacaa
      421 aactgcccgg cgccagttcg ccggcaaaga tctgctggcg cagcttctcg gcggcctggt
      481 ggtgcaagga gtcggcaatg ctgcgcatgg tggcgattca cggagcccgg tggccctcca
      541 atgacaggca gaacaacgcg aaaacatgtg atttcgcata atttttcata attatggcga
      601 tccaggactc gcaccatgat cggtgctatc ccctacggcc ggatccatgg tgccatccgc
      661 caggacagtg aagccggctt ttgcgccctt gtctttgccg ggatggcccc taccatccca
      721 tcatcgcgcc gcagtcaggc tggcgcccac tttgcggcag gccggcgcaa gcccgtcgcc
      781 gcggctacac cggagtgtgt gatgacccaa aacacccaca agaccgtgct ctccacccgc
      841 agcgcgccag agcgccactg ggtcggcgac ggctttcccg tgcatggcct gtttggctac
      901 agcggcgcgg gcgtggccga gcgcagcccc tttctgctgc tggactacgc cgcgcccacc
      961 accttcacgc ccaccacggc gcgccgcggg gtgggcagcc acccgcaccg gggcttcgag
     1021 accgtgacca tcgtctacga cggcgaggtg gagcaccgcg actccaccgg cgccggcggc
     1081 atcatcggag cgggcgacgt gcagtggatg accgccggcg gcggcatcat ccacgaggag
     1141 ttccactcca gcgcctacgc cgccagcggc ggcccgttcg agatggtgca gctgtgggtc
     1201 aacctgcccg ccagccagaa gatgacgccg gcgcgctacc aggccatcct ggacgcgcag
     1261 atcccggccg tggcgctgcc gggtggcgcc ggccgggtgc gcgtgatcgc cggcagcttc
     1321 gacggccggc aggggccggc gcacagcttc acgcccatga atgtgctgga catgcgcctg
     1381 gcggccggcc accggctgca gctggcgcaa cccgagggct ggagcacgct ggtcgtggtg
     1441 ctggccggca ctgtgcagat caatgacggc acgacgctgg gcgccgccga gatggccagc
     1501 ttgtccacac agggcacgca gctgaccctg gccgcgcaag aagatgccaa gctgttgctg
     1561 ctggcgggcg agcccatcgc cgagccggtg gtcggctacg ggccgttcgt gatgaacagc
     1621 gagcagcaga tcgcgcaggc catcgacgac ttcaaccgcg ggcatttcac ccattgaaaa
     1681 ggagccgtgc catgaccgac agccaacgcg aaacaccgcc gcagcgctac ggcgcacgcg
     1741 ccaccgtgat cggcgaggac acgcgcatcc tgcgtgccct gccgcaccgc gaacggcgca
     1801 ccgtgggcgc ctggtgtttt ctggatcacc tggggccggt gcagttcgcg ccgggcaagg
     1861 gcatgcatgt cggggcgcac ccgcatatcg gcctgcagac cttcacctgg atgatcgagg
     1921 gcgaggtggt gcaccgtgac tcgctgggca acgagcagac cattcgcccc ggccaggtga
     1981 acctgatgac cgccgggcgc ggcatagcgc ataccgagga ttcgctgctg gagggcgcgc
     2041 gcctgcacgc cgcgcaactg tggattgccc tgcccgacgc gcagcgccat tgcgcgccgg
     2101 cattcgagaa ctaccccgag ctgccccagg tggacgccgg cggctggcgc gccaccgtgc
     2161 tcgccggcag cgccctgggc cagacggcgc cggcccaggt ctacacaccg ctggtgggcc
     2221 tggacatggc cagcaccggc ccggcccaga cccaactgcc tctgcaaccc gggtttgaat
     2281 acgccgccat ggtgctgcgc ggcgcggcca cggtggccgg cgagccactg gcgccgggcg
     2341 agtggctgta cttcgcgccc ggccgcacga ccctcgatgc gcgctgcgac gccgcggcgc
     2401 aatggctgct gctgggtggc gagcccttgg gcgaggacat cctcatctgg tggaacctgg
     2461 tggcgcgcaa ccaggccgag ctgcaaagcg cgctggacga ctggaacgcc ggccgctttg
     2521 ccccggtgcg ccccggcagc ccggccgaac ccctgcccgc ccccagcctg agtggcgcac
     2581 gcgtgcgcgc cagcggccag gcctgatgcc cgcgctggcc taccgccagg taggttgttt
     2641 actataatat ttatagctgt ttgcgcttat ccataaattg ctacagccat ttttttatct
     2701 gattatggac aggccttgga ttgggcctgg aagccgcatc gttggcaacc caaccacgcc
     2761 ctgcagcgcc tgcgagtttc ataggtgaca gacagggcct ggacgccccc atagcatcca
     2821 cccccatgcc ccagtggccc atccgcctgg ccacagccag ggttcatgag cacccttccg
     2881 ccagcatctg caccacccat ggctccatcg ccctgggtgc tgctgcagcc ccagccttcc
     2941 aaggagacaa agcgatgagc gtgaagcaac ttttcgatct gaccggccag gtggccctgg
     3001 tcaccggcgg ctcgcgcggg ctgggcctgc agatggccca ggcgctgggc gagatgggcg
     3061 ccaggctggc catcacggcg cgcaaggccg acgaactggc ccaggccagg gcgcagctgg
     3121 agggcctggg ctacgaggtc gagaccatag tcaacgacct ctcaaagttc gagcagatac
     3181 cggccatggt cgatcaggtg ctggcacgct ttggcaccat cgacatcctg gtcaacaacg
     3241 ccggcgccac ctggggcgcc aaggccgagg actaccccga cgccgcctgg aacaaggtga
     3301 tggatctgaa cgtgaacgca cccttcttcc tctcgcgcga ggtgggcagg cgcgtgatga
     3361 tccccaaggg ccggggcaac atcatcatca ccgcctcggt ggccgcgctc aagggcacgc
     3421 cgccgggcat gaacaccatc gcctacaaca cctccaaggc cgcggcgctg cacttcgcgc
     3481 gcacgctggc gtcggaatgg gggccctacg gcatccgcgt gaacgccatc tgcccgggtt
     3541 tctttccgag caagctggcc agcggcctga tcgacaagct cgggccagca ctgatcgagc
     3601 gcacgccgct gcgccgcatt ggcggcgacg aggatttgat gggcgcggtg gtgttcctgg
     3661 cctcgaacgc ctcgcgccac atcaccggcc aggggctggt cgtggacggc ggcgccagcc
     3721 tgatctgatt atttaaaagg gactctctca tggacttctc ctactccccc aaggtcgtcg
     3781 atctgcaaca gcgcctcacg gccttcatgg atgaacatat ctacccggcc gaggccgtct
     3841 accacgccga ggtggaggcc aaccgccgcg ccggcaacgc ctggcaaccc acgcgggtga
     3901 tggaggactt gaaggccaag gcccgcgccg ccgggctgtg gaacctgttc ctgcccgaat
     3961 cccaatacgg cgccggcctc acaaacctgg aatacgcgcc gctgtgcgaa atcatgggcc
     4021 gctcgcatgt ggcgcccgag gccttcaact gctcggcgcc cgacaccggc aacatggaaa
     4081 ccctggtgcg ctacgccacg cccgagcagc aacagcgctg gctgctgccg ctgctggacg
     4141 gcaagatccg ctcggccttc gccatgaccg agcccgccgt ggcctcgtcg gacgccacca
     4201 acatcgaggc gcgcatagag cgcgtgggcg acgaatacgt gatcaacggc cgcaagtggt
     4261 ggacatcggg cgcgcccgat ccgcgctgcg agatcctgat cttcatgggc aagaccgacc
     4321 ccggccacgc cagccgccac cgccagcagt ccatgatcct ggtgcccatg aaaaccccgg
     4381 gggtgaccat ggagcgcgcg ctgtccgtct ttggctacga ccacgcgccg cacggccacg
     4441 gcgaggtgag ctttgacaac gtgcgcgtgc cggtcgccaa cgtgctgctg ggcgagggcc
     4501 gcggcttcga gatcgcccag gggcgcctgg gccccgggcg catccaccac tgcatgcgcc
     4561 tgattggcgt ggccgagcgc gcgctggagc tgatgtgccg gcgcgccttg ggccgcgtgg
     4621 ccttccaccg tccggtggcc gaccagggcg tgacacgcga gcgcatcgcc aacgcgcgca
     4681 tcctgatcga ccaggcgcgc tttctggtgc tcaacgccgc ctacatgatg gacaccgtgg
     4741 gcaacaaggt cgcgcaaaaa gaaatcgcca tgatcaaggt ggccgccccc agcatggcct
     4801 gccaggtgat cgactgggcc atgcaggtgc atggcggcgc cggcgtgtcg gacgactttc
     4861 cgctggccgc cgcctacgcc catgcgcgca cgctgcgctt tgccgacggc cccgacgagg
     4921 tgcaccgcaa ccagatcggc aagatggagc tggcccgcta catggcgctg gacaagcgct
     4981 gaggcacgcc atgtccaagc gccacctggc ccattggccg cgcctgctgc cgcagcacct
     5041 gacactgccg gccacgcacc tgtaccgcaa cctcgaggtc tcggccatgc gctacccgga
     5101 caaggcggcg ctggtgttct acgacgcggc catcagctat gcgcgcctgc aggacgagag
     5161 cgagcgcctg gcggcccacc tgcagcagcg ctgcggcgtg cgccggggtg accgcgtgct
     5221 gctgtacctg cagaacagcc cgcagttcgt cattggctac tacgccatcc tgcgcgccga
     5281 cgcggtggtg gtgccggtga acccgatgaa cctgagcgag gagctgcgcc actacgtgct
     5341 ggatgccggc gcgcgcacca tcctcgcgcc gcaggacttg tgggcccagg tgcgcccgct
     5401 gctgcacgaa ggcctggcgg ccgccgctgc cgatgcgcaa gcgccgggcc tgcgccatgc
     5461 cgtggtggcg gcctatggcg actacctgac ggcgcccacc gacctggccg tgccggcctt
     5521 cgtcagcgcg ccgcgcgagc cggtcgaaga acccggcgcc tgcgcatgga tggacgccct
     5581 ggccgccggt ctgcgccccg ggcccatcgc cgccggcccc gacgatctgg ccgtgatgcc
     5641 ctacacctcg ggcaccacgg gccagcccaa gggctgcatg cacacgcacc gcagcgtcat
     5701 gtacaacacg gtctcgggcg gcatctggtt gggcacgacg ccggacgcgg tgtcgctgac
     5761 ggtgctgccg ctgtttcatg tcacgggcat gcagggcggc atgaacagcc ctttgtatac
     5821 cggctccacc atcgtgctgc tgccgcgctg ggatcgcgac gcggcggcgc agctgatagc
     5881 gcgctaccgc gtgacgaaca cgcagatgat cgtcaccatg gtggtggacc tgctgtccaa
     5941 cccgcgcctg gccgagtacg acctgtccag catccggcgc ctgagcggcg gcggcgcggc
     6001 catgcccgag gccgttgcgg ccaagctcca ggagctgtgc cagatcgact acatcgaggg
     6061 ctatggcatg tccgagacca tggccgccac gcatatcaac ccgcccgaca ggccacgcaa
     6121 acaatgcctg ggcataccca tcttcgacgt ggatgcgcgc gtggtcgatc ccgccacctt
     6181 cacggaacta ccgcccggcg aggtgggcga gatcatcgtc catggcccgc agctcatgca
     6241 gggctactgg ggccaggccg aggccacggc ccaggccttc gtcactctgg atggcaagcg
     6301 cttcctgcgc accggcgacc tggcgcgcac cgacgacgat ggctatttct acatggtgga
     6361 caggctcaag cgcatgatca atgcctcggg ctacaaggtc tggccggccg aggtcgaggc
     6421 cctgatgtat gcccacccgg cgatccagga ggtctgcgtg atcgccgcgc aggacgcgcg
     6481 ccgcggcgag accgtgaagg ccgtggtggt gttgcgcgag gccttcaggg gccaggtgag
     6541 cgagcaggac atcgtggact gggcccatgg ccacatggcg gcctacaaga gcccgcgcct
     6601 ggtctcgttc gtggacagcc tgcccaagtc cggcaccggc aaggtgcagt ggcgcgccct
     6661 gcaggagcgc gagtcggcgc cgcgctgacg ggccagcgtg cccgcctata ctggctgcca
     6721 cggcggccag gaatgggggc gcagcgcccc ttgcgccttc ttgcagcggg aggttttcat
     6781 ggacaggttc cagttttgcg ccagtctcgt ctgcggcgcg gcccttgccg ccctgccccg
     6841 tgcggcattc gcccaggcga cgcctgcgca gcggctggat acgccccggg ccatcgacat
     6901 ggcggggcgg ctgcgcatgc tgtcgcagcg ctgcaccaag gcctatctgc aatggggtca
     6961 gtcgattgcc ggcgacagcg ctcgcacgct gctgcaagag tccgtcagcc ggtatgaagc
     7021 ccacctggcc gcgctcaaga ccttcatgcc caacccggcg gtgcaggcca gcctgccgca
     7081 gctgcaaacc ctctggtcgc aattcaaggc gcttttgacc ggagagccca gccgcacggg
     7141 cgcagccgcg ctgtacgacg ccaacgagac gctgcaaagc gcggcccact acctcaccgt
     7201 gtcgtacggc aacaccaacg gcacgccgct ggaatacctg atcggcctgg ccgggcgcca
     7261 gcgcatgctg tcgcagcgca tggccaagtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn gcgcgggcgg cgcgcataca gccacagcag cacgaacagc aacaggccct
     7441 ccagcaggaa ctggtagatc tgcgacgggt ggcgcggcag catggagccg ctttgcggaa
     7501 acaccatggc ccagggcagg tcgggcgagg ccatgcgccc ccacagctcg ccgttgatga
     7561 agttgcctat gcgcccggcc gccagtccgg tgggcacgca gggcgcgaca aagtccgcca
     7621 cctgcagcca gggcctgtgg cgcgagcgcg caaaccacag catggagccg atcacgccca
     7681 agagcccgcc atgaaagctc atgccgccct gccacacgta gaagatctcc agcgggtgcg
     7741 tcaggtagta gccgggcttg tagaacaggc agtagcccag ccgcccgcct atcaccaccc
     7801 ccagcacgcc caggaacaga atgtcctcca catccttgcg cgaccaggcc tgctcgcccg
     7861 tgagcgaggc atagggctcg tgccgcaggc ggcgcaggcc cagcaacatg aacaggccaa
     7921 aggcggccag ataggtcagg ccataccagt gaatggcaac gggcccgatc tgcagggcca
     7981 cggggtcgat ctgcgggtac atcagcatgg cggcgattgt gccgcagctg tatggcggcc
     8041 aaatttgaat cgccgcccgt ggcgcgcgat actcgggacc acagcaaccc acgacagagc
     8101 aacaccatgc aaggccatac cgccgtcatc gactgcctca aggaactgct gcgcggcgaa
     8161 ctggctgcgc gcgaccagta cttcatccac tcgcgccagt acgaggatca gggcttcgtg
     8221 cgcctgtacg agcgcctgaa ccacgagatg caggaagaga ccgagcatgc cgacgccatc
     8281 ctgcgccgca tcctcatgct ggagggcaag cccgacatgc gcccgcacgc cttcacgccg
     8341 ggcgagagcg tccaggagat gctgcgcaag gacctggaca ccgaatacag cgtgcgcaag
     8401 cacctgaagt cggccatcgc cctgtgcgag tcacaccacg actatgtgag ccgcgacctg
     8461 ctgctgaccc agctgcgcga caccgaggaa gaccacgcct attggctgga aaagcagatc
     8521 ggcctgatcg acaaggtggg cctgcagaac tacctgcaat cgcagaccgg ttcgtagtca
     8581 ggcgttcgcc gggacattga ccatggtggc ctgcatcagg gccaccagct tgtactgcgg
     8641 cccatcgccc gcaaacgcac gcacctcgcc cgtgcacacg gtgagcatgc gccccgcgtt
     8701 ctgcacccgg cccacggcca ggaagcgcgc cccgatggcc ggccgcatga agttggtctt
     8761 gaactccgct gtgacgatct cgcagccagg cggtgacctg gtcagcgccg catagccgca
     8821 ggcgttgtcc acgatgctgg tgacgacacc cgcatgcacg aagccctgct gctgcgtcag
     8881 gtgcggcgag aacggcagct cgatatgcac ctcgccgtcc tgcaccagca ccaggcgtgc
     8941 gcccagcgtg gtcatcaggc cctgggcgtt gaagctggcg gcaatgcgct ggtgcaactc
     9001 ggacatgccc gtcaatccag cagcgacagg tcgcgcacgg cgccggtgtc ggcgctggtg
     9061 accagcttgg cataggcctt gagcgccgcc gagaccttgc gcggacgcgg ctgggccggc
     9121 ttccagccca gggcagcctg ctgttggcgc cggcgcgcca gttcctcgtc gctgaccagc
     9181 acgttgatcg tgcggttggg gatgtcgatg cggatgcggt cgccgtttcg caccaggccg
     9241 atggcgccgc cggccgcggc ctcgggcgag cagtggccga tcgacaggcc cgaggtgccg
     9301 cccgagaagc gcccgtcggt cagcagcgcg caggccttgc ccaggccctt gctcttgatg
     9361 tagctggtgg ggtagagcat ctcctgcatg ccggggccgc ccttggggcc ttcgtagcgc
     9421 accacgacca cgtcgcccgc cttgaccttg tcggccagga tgttggccac ggcctcgtcc
     9481 tgcgactcga ccacatgcgc cgggccctcg aacaccagga tggagtcatc cacgcccgcg
     9541 gtcttcacca cacagccctt ttgggcgatg ttgccggtga gcacggccag gccgccttcc
     9601 ttgctgaagg cgtggtcgta ggagcgtatg cagccctcgg cgcggtccag atccaggctg
     9661 ggccagcggc tcgcctggct gaacgcaacc tgggtgggaa tgccccccgg gccggccttg
     9721 aagaagttgt gcacggcctc gtcctgcgtg aggcagatgt cccattgcgc aatcgcctcg
     9781 cccagggtgg gcgcgtggat ggtgggcacg tcggtatgca ggcggccggc acgttccagc
     9841 tcgcccagga tgccgaagat gccgccggcg cggtgcacgt cctcgatgtg gtacttgtcg
     9901 gtgttgggcg ccaccttaca cagctggggc acgacgcgcg agaggcggtc gatgtcgtcc
     9961 atggtgaagg caatctcggc ctcctgcgct atcgccagca ggtgcaggat ggtgttggtg
    10021 gagccgccca tggcgatgtc cagcgtcatc gcattctcga aggccttgaa acccacggcg
    10081 cgcggcagga tgcgctggtt gtcctgctcg tagtattcgc gcgccagctc cacgatgcgc
    10141 cggccggccc gcttgaacag ctgttcgcgg tcggcatgcg tggccagcac cgtgccatta
    10201 ccgggcagcg acaggcccag cgcctcggtc aggcagttca tcgaattggc ggtgaacatg
    10261 cccgagcagg agccgcaggt ggggcaggcc gaacgctcca cctcggccac gtcggcgtcg
    10321 ctggccttgt cgtcggcggc catgaccatg gcgtcgatca gatccagctt cttggtgaac
    10381 gtcagcttgc cctgccccgg cacggccagc tgggccttcc cggcctccat ggggccgccg
    10441 gagacgaaga tcaccggaat gttcaagcgc atcgccgcca tcagcatgcc gggcgtgatc
    10501 ttgtcgcagt tgctgatgca gaccatggcg tcggcgcaat gtgcgttgac catgtactcc
    10561 acgctgtcgg caatgatgtc gcggctgggc agcgagtaca gcatgccgtc gtggcccatg
    10621 gcgatgccgt cgtccacggc gatggtgttg aactccttgg ccacgccgcc ggcggcctcg
    10681 atctcgcggg ccaccagctg gcccagatcc ttcaggtgca catggccggg cacgaactgc
    10741 gtgaacgagt tgaccaccgc gatgatgggc ttggagaagt ctgcatcctt catgccggtg
    10801 gcgcgccaca gggcgcgggc accggccatg ttgcggccat gggtggaggt cttggaacgg
    10861 tatgcgggca tggtgattgc aaaaaaagga atgtctcggg gggatgaatg cgacggcccg
    10921 cgcaattgca ggccggtgta caccatggcg cgcgcctctt gagccgggcc atgggcaaaa
    10981 ccatttcatt atgtcgcagc ggctggacag tcgcaggcca ggcgcccaca cttgcaggcc
    11041 ccagccacag cgccaagacc atcatgaccg accctctgcc actgaaccgc cgctccgccc
    11101 atatcaccca gggcaaggcg cgcgcagcaa accgctccat gtactacggc atgggctaca
    11161 ccgaggccga cttcaacaag cccatggtcg gcgtggccaa cgggcacagc accatcacgc
    11221 cctgcaacag cggcctgcaa aggctggccg acgcggcggt ggacggcata cgcgcggcgg
    11281 gcggcaacgc ccagaccttt ggcacgccca ccatcagcga cggcatggcc atgggcaccg
    11341 agggcatgaa gtacagcctg g
//
LOCUS       DAOMTQ010000101        11120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1106_length_11120_cov_27.535563, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000101 DAOMTQ010000000
VERSION     DAOMTQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1106_length_11120_cov_27.535563"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK518_10410"
     CDS             <1..282
                     /locus_tag="PK518_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49370.1"
                     /translation="PRPTALVPPTVPEVAPGSTRFDPIEADEVAAFKQALVNAAGRAP
                     QAPVEPGVATRSGPLLPPSPALTGFEDTVMPSQEGRSPDLSSTQYGDLR"
     gene            complement(294..848)
                     /locus_tag="PK518_10415"
     CDS             complement(294..848)
                     /locus_tag="PK518_10415"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRM49371.1"
                     /translation="MPHLNVTHYLRQHIRTVPNWPAPGVQFRDITPLLQNPKVFRVLI
                     DAFVQRYMDPALRPDVVAGLDARGFILGSVVAYELGLGFVPIRKKGKLPFTTVEETYE
                     LEYGSATVELHADAVQPGDRVLLIDDLIATGGTMMAGKKLLEKLGASVMEGAAIVDLP
                     ELGGSARLRASGLPLFTLVDFGGH"
     gene            complement(889..2871)
                     /locus_tag="PK518_10420"
     CDS             complement(889..2871)
                     /locus_tag="PK518_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRM49372.1"
                     /translation="MSSLALTLLYLLAAVLGVVACRSLRLPPMMGYLAAGILIGPHAL
                     ALTQNSEGVRHLGEFGVVFLMFTIGLEFNLPKLRAMRRHVFGLGLLQVLLSMLAFTAC
                     LLLLARLGGVWDMGWQTALALGGTLAMSSTAIVVKLMSERGELESEHGRRVLGILLFQ
                     DLAVVPLLVLIPALGSSPDKLLLALGLALLKAMVLVSVLLVGGQGVMRWWLTLVARRK
                     SDELFMLNLLLVTLGLAWMTELAGLSLALGAFIAGVLVSETEFRHQVGTDIRPFHDVL
                     LGLFFITVGMMLDWHVMVERWALVLLLLAAPLLIKTAIIVALARGLGATTGVSLRTAL
                     YVAQAGEFGFVLLSLMQTHELLAPALMNPVLAAMVLSMLATPFLIQYSNRIVMKLVAS
                     DWLQQSLQVTSIARQAINTSGHVLICGYGRCGQNLARMLEREGIPYMALDLDPDRVRQ
                     AAAAGDSVVFGDATRVQALKAAGLGRAAALAITYLDTAAALKVLANARAHAPQVPVVV
                     RTEDDTHLEKLQAAGATEVVPEAIEGSLMLAGHALALVGVPMRRVLRLVQGQREQRYG
                     LLRGYFHGADDDTVSERDQERLYSITLPPGAAAVGASLATLTLHAMGARLINVRRFSG
                     RMSGPDDEAPLAEGDTLVLSGHPTALSLAEEKLLRG"
     gene            2920..3921
                     /locus_tag="PK518_10425"
     CDS             2920..3921
                     /locus_tag="PK518_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRM49373.1"
                     /translation="MPNAPSAPPPFDAAQALRLGRETFDIEAQALQSLGARLGQVFVQ
                     AVQLLLATPGRVVVMGMGKSGHVGRKIAATLASTGTPAFFVHPAEASHGDLGMVTRGD
                     LLLALSNSGESSEITVLLPMLKRQGVPLIAMTGGLQSSLARHADLVLDCSVEREACPL
                     NLAPTTSTTVQLAMGDALAVALLDARGFRPEDFARSHPGGALGRRLLTHVRDVMRSGE
                     QVPQVLPQVDFSSLMREMSAKGMGAAAVVDEAGRPIGIFTDGDLRRRIEAGADLRGMR
                     AQDIMHANPRTIAADALAADAAQLMEQHSITSVLVTEPGGVLVGVVHIGDLMRAKVI"
     gene            3921..4487
                     /locus_tag="PK518_10430"
     CDS             3921..4487
                     /locus_tag="PK518_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HRM49374.1"
                     /translation="MQQPLPPLRPVLQFAPALLLRAQDVRVAFFDVDGVLTDGGLYFS
                     EAGETLKRFNTLDGHGLKLLQQAGITPAVITGRDSPALRLRLAALGVVHARFGTEDKR
                     PAAEAILAELGLAWGQAAAMGDDWPDLAVMRRAAFACAPPQAQAEVRAIAHHVTAAAG
                     GAGAARELCDLLLVASGRYAGLLAAHGA"
     gene            4484..5101
                     /gene="lptC"
                     /locus_tag="PK518_10435"
     CDS             4484..5101
                     /gene="lptC"
                     /locus_tag="PK518_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRM49375.1"
                     /translation="MMSSLRRAWERLSLYLPVLLMGLLALGTWWLVRNAPQPQPAVVE
                     RPVSHEPDYFMRDFAVKNFDATGRLQSDIRGQLARHYADSDQLEIDQARMRSVSPEGR
                     VTTASANRALTNADGSEVQLFGNAIVTREPLVRKGQATLPRLEFRGEFLHAWVNDERV
                     RSNQPVTLKRGSDTFTADSMDYDNLDQILNLRGRVHGTLQPAARR"
     gene            5117..5902
                     /locus_tag="PK518_10440"
     CDS             5117..5902
                     /locus_tag="PK518_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM49376.1"
                     /translation="MPARPLVFITGASSGIGRALAERYHRAGWRLALVARRELEIKSW
                     AGAHQLSASSYQIYGADVAQMDSIIAAGQACLQHQGLPDVVIANAGISVGMDTAERAD
                     LDVMARVFATNNLGLAATFHPFISAMTQRGSGRLVGIGSVAGIRGLPGHGAYCASKAA
                     VISYCESLRGELRASGVRVVTISPGYVDTPLTRENRYRMPFLMTPEAFAESAFKAIET
                     GRSYRVIPWQMGVAAKLLRLLPNPWFDRLLQGRPRKHRQPPSS"
     gene            complement(5951..6433)
                     /locus_tag="PK518_10445"
     CDS             complement(5951..6433)
                     /locus_tag="PK518_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRM49377.1"
                     /translation="MGNKLYVGNLPYSVRDQDLEQAFGQFGTVTSAKVMMERDTGRSK
                     GFGFVEMGSDAEAQAAINGMHGQPLGGRAIVVNEARPMEPRPPRSGGGGFGGGFGGGG
                     GGGYGGGRGAGGGGGGRGEGGFRSPYGSGPRGGGGGGGGRGGYGGGGGGGYGGDRGGY
                     "
     gene            complement(6671..7039)
                     /locus_tag="PK518_10450"
     CDS             complement(6671..7039)
                     /locus_tag="PK518_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRM49378.1"
                     /translation="MGNKLYVGNLPYSFRDQDLEQTFGQFGAVQSAKVMMERDTGRSK
                     GFGFVEMGSDAEAQAAIQGVHGQNFGGRDLVVNEARPMEPRPPRSGGYGGGGGYGGGR
                     GNGGGYGGDRGGYGGGRSSY"
     regulatory      7289..7407
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            7511..8089
                     /locus_tag="PK518_10455"
     CDS             7511..8089
                     /locus_tag="PK518_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRM49379.1"
                     /translation="MEAFLISTSIVALAEMGDKTQLLSLVLAARFRKPWPIVLGILVA
                     TLANHALAGAVGAWVTTVLGPQALRWVLGLSFIAMAVWMLIPDKLDDDGEGKGVRLGV
                     FGTTVVAFFIAEMGDKTQIATVMLAAQYNAYLWVVAGTTLGMMIANAPVVWLGDRLVK
                     KVPIRLVHIISAVIFLVLGLLALFVPQATPLA"
     gene            8205..9344
                     /locus_tag="PK518_10460"
     CDS             8205..9344
                     /locus_tag="PK518_10460"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRM49380.1"
                     /translation="MSFIATPQSMHFAEALRLQSGASIRDYTLAYETYGELNAARDNA
                     VLVCHALNASHHVAGVYEGQPKSEGWWDNMIGPGKPLDTNRFFVIGINNLGSCFGSTG
                     PMHAHPDTGEVYGADFPVVTVEDWVNAQALLLDRLGVAQLAAVMGGSLGGMQALSWTL
                     QYPERVRHAVVVASAPNLTAENIAFNEVARRAIVTDPDFHGGHFYRHGVIPQRGLRIA
                     RMIGHITYLSDDVMNEKFGRQLREGMDLKYSTQDIEFQIESYLRYQGDKFSEYFDANT
                     YLLITRALDYFDPARRHGGNLAQALAVARAKFLLVSFSTDWRFSPQRSREIVQALLEN
                     RRDVSYAEIDAPHGHDAFLLDDARYMGVVRSYFDSISKELQGGQP"
     gene            9341..9925
                     /gene="metW"
                     /locus_tag="PK518_10465"
     CDS             9341..9925
                     /gene="metW"
                     /locus_tag="PK518_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRM49381.1"
                     /translation="MSDHATMQAIARLVPEGSRVLDLGCGDGALLDLLQRERGCSGYG
                     IELADANVLACVRRGVNVIQLNLDEGLAMFGDNSFDVVLQIDTLQHLRNAEVMLRETA
                     RVGQSGIVAFPNFAHWPNRLSVLRGRMPVTRRLPYQWYDTPNIRVGTYKDFEVLAYKN
                     KLRILDAFGLQDGREVRWLPNALAGTAVFRFEHD"
     gene            9991..>11120
                     /locus_tag="PK518_10470"
     CDS             9991..>11120
                     /locus_tag="PK518_10470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM49382.1"
                     /translation="MLPLDVPTMLLMTAAASATMALSMAAVRPERREGMGLWALALVL
                     HTITYLLFTLRGAVPAWASVVLANTLLAGTFALALAAVRQFQGHGLPWRRMLVPVLAM
                     ALLFAWFQDDYRACVIVAGVVPPLQVALVLWALWRPKPPAQMRGAVLLTAGLGLQAVL
                     LAVRGALAALHSIPTQGLLRTDGVQSLTFMAAFVVVILASLGFILMAKDRADANNHYF
                     ATRDALTGLFNRRALLLAARRDVAVAARSHEPYALMMVDIDHFKAVNDGHGHQVGDQI
                     LCHVAGLLSARLRAQDMVGRYGGEEFLVLLPNTAQQAAAELAETLRSAVAQCPCAREG
                     CAITATVSIGVCGGQLHGTEDWERLLRCADQALYAAKAAGRNR"
BASE COUNT         1837 a         3855 c         3685 g         1743 t
ORIGIN      
        1 ccacgcccga ccgcattggt gccgccgacg gtgccggaag tggcgccggg cagcacgcgc
       61 ttcgacccca tcgaggccga cgaggtcgcg gccttcaagc aggcgctggt gaatgccgcc
      121 gggcgcgcgc cccaagcccc ggtggagccc ggcgtggcca cgcgcagcgg cccgctgctg
      181 ccgcccagcc cggcgctgac cggcttcgag gacacggtga tgcccagcca ggaggggcgc
      241 agccccgacc tgagcagcac gcagtacgga gatctgcgct gaaaacggcg gcctcaatgg
      301 ccgccgaagt ccaccagggt aaacagcggc agtccgctgg cgcgcaggcg cgccgagccg
      361 cccagctcgg gcagatccac aatggccgcg ccctccatga cgctggcgcc cagcttttcc
      421 agcagtttct tgcccgccat catggtgccg ccggtggcga tcaggtcgtc gatcagcagc
      481 acccggtcgc cgggctggac ggcgtcggca tgcagctcca cggtggcgct gccgtattcc
      541 agctcgtagg tttcctccac cgtggtgaag ggcagcttgc ccttcttgcg gatgggcaca
      601 aagcccaggc ccagctcata ggccacgacc gaacccagga tgaaaccgcg cgcatccagc
      661 cccgccacca cgtccgggcg cagggccggg tccatgtaac gctgcacgaa ggcatctatc
      721 agcacgcgaa acaccttggg gttctgcagc agcggcgtga tgtcgcgaaa ctgcacaccc
      781 ggcgccggcc agttgggcac ggtgcggatg tgctggcgca ggtagtgggt gacgttgaga
      841 tggggcatgg cttgtcgcag gtcgggtcag gggcggcgca aagtgtaatc agccgcgcaa
      901 cagtttttct tccgccaggc tcagggccgt ggggtgaccc gacagcacca gggtgtcgcc
      961 ctcggccagt ggcgcctcat cgtcggggcc ggacatgcgc ccgctgaacc ggcgcacgtt
     1021 gatcaggcgc gcgcccatgg cgtgcagcgt cagcgtggcc aggctggcgc ccaccgctgc
     1081 ggcacccggg ggcagggtga tggagtacag gcgctcctgg tcgcgctcgc tgacggtgtc
     1141 gtcatcggcg ccgtgaaaat agccgcgcag caggccatag cgctgctcgc gctggccctg
     1201 caccaggcgc agcacgcggc gcatgggcac gcccaccagg gccagggcat ggccggccag
     1261 catcagcgag ccctcgatgg cctcgggcac gacctcggtg gcgccggcgg cctgcagctt
     1321 ctccaggtgg gtgtcgtcct cggtgcgtac caccaccggg acttgcggcg catgggcgcg
     1381 ggcattggcc agcaccttca aggcggcggc cgtgtccaga taggtgatgg ccagggccgc
     1441 ggcccggccc aggccggcgg ccttcagagc ctggacgcgc gtggcatcgc caaacaccac
     1501 cgagtcgccg gcggcggcgg cctggcgcac gcggtccggg tccagatcca gcgccatgta
     1561 ggggatgccc tcgcgttcga gcatgcgcgc caggttctgg ccgcagcggc catagccgca
     1621 gatcagcaca tggccgctgg tgttgatggc ctggcgcgca atgctggtca cctgcagcga
     1681 ctgctgcagc cagtcgctgg ccaccagctt catgacgatg cggttgctgt actggatcag
     1741 gaagggcgtg gccagcatgg acagcaccat ggccgccagc accgggttca tgagcgccgg
     1801 cgccagcagc tcgtgcgtct gcatcagcga cagcagcacg aagccgaatt cgcccgcctg
     1861 cgccacgtac agcgcggtgc gcagcgacac gccggtggtg gcgcccaggc cgcgcgccag
     1921 ggccacgatg atggcggtct tgatgagcag cggcgccgcc agcaagagca gcaccagcgc
     1981 ccagcgctcc accatcacat gccagtccag catcatgccc acggtgatga agaacaggcc
     2041 cagcagcacg tcgtggaagg ggcggatgtc ggtgcccacc tggtggcgga actccgtctc
     2101 ggacaccagc accccggcga tgaaggcgcc cagcgccagc gacagcccgg ccagctcggt
     2161 catccaggcc aggcccagcg tgaccagcaa caggttcagc atgaacagtt cgtcgctctt
     2221 gcgcctggcc accagggtca gccaccagcg catcacgccc tgaccgccca ccagcagcac
     2281 gcttaccaac accatggcct tgagcagggc caggcccagc gccagcagca gcttgtcggg
     2341 tgacgagccc agcgccggta tcagcaccag cagcggcacc acggccaagt cctggaacag
     2401 caggatgccc agcacgcgcc ggccatgctc gctctcgagc tcgccacgct cggacatcag
     2461 cttgacgacg atggccgtgc tgctcatggc cagcgtgccg cccagggcca gggcggtctg
     2521 ccagcccatg tcccacacgc cgcccagtcg tgccagcagc agcaggcagg cggtgaaggc
     2581 cagcatggac agcagcacct gcagcagccc caggccgaac acatggcggc gcatggcgcg
     2641 cagcttgggc aggttgaact ccaggccgat ggtgaacatc aggaacacca cgccaaactc
     2701 gcccaggtgg cgcacgccct cggagttctg cgtcagcgcc agcgcatgcg ggccaatgag
     2761 gatgcccgcc gccagatagc ccatcatcgg cggcagccgc aggctgcggc aggcgaccac
     2821 gcccagcacg gcagccagca ggtacagcag cgtcagggcg agtgaggaca tgcctgatcc
     2881 tagccgagcc gggggcgggc cgaccgatag aattcgccca tgcccaacgc cccatccgcc
     2941 cccccgccct tcgatgccgc gcaggccctg cgcctggggc gcgagacctt cgatatcgag
     3001 gcccaggcat tgcagagcct gggcgcgcgc ctgggccagg tgtttgtgca ggcggtgcag
     3061 ctgctgctgg ccacgcccgg gcgcgtggtc gtcatgggca tgggcaagag cggccatgtg
     3121 gggcgcaaga tcgccgccac gctggcatcc accggcacgc ccgcgttctt cgtgcacccg
     3181 gccgaggcca gccatggcga cctgggcatg gtcacgcgtg gcgacctgct gctggccctg
     3241 tccaacagcg gcgagagcag cgaaatcacc gtgctgctgc ccatgctcaa gcgccagggc
     3301 gtgcccctga tcgccatgac gggcgggctg cagtcgtcac tggcgcgcca tgccgacctg
     3361 gtgctggact gcagcgtgga gcgcgaggcc tgcccgctga acctggcccc gaccaccagc
     3421 accacggtgc agctggccat gggcgacgcg ctggccgtgg ccctgctgga cgcgcgtggc
     3481 tttcgtcccg aagactttgc ccgctcgcac cccggcggtg cgctggggcg gcgcctgctg
     3541 acccatgtgc gcgacgtcat gcgcagcggc gagcaggtgc cgcaggtact gccgcaggtg
     3601 gacttcagct ccctgatgcg cgagatgagc gccaagggca tgggcgccgc agccgtggtg
     3661 gacgaggcgg gtcgccccat cggcatcttc accgacggcg acctgcgccg gcgcatagag
     3721 gccggcgccg acctgcgcgg catgcgcgcc caggacatca tgcatgccaa cccccgcacc
     3781 attgccgccg acgcgctggc ggccgacgct gcgcagctga tggagcagca ctccattacc
     3841 agcgtgctgg tcaccgagcc cggcggtgtg ctggtgggcg tggtgcatat cggcgacctg
     3901 atgcgcgcca aggtgatctg atgcaacaac ccctgccccc gctgcgcccc gtgctgcaat
     3961 tcgccccagc gctgctgctg cgtgcacagg atgtgcgcgt ggcctttttc gacgtggatg
     4021 gcgtgctcac cgacggtggc ctgtatttct ccgaagccgg cgagaccttg aagcgcttca
     4081 acaccctgga cggccatggc ctgaagctgc tgcagcaggc cggcatcacc ccggcggtga
     4141 tcaccgggcg cgattccccc gcgctgcgcc tgcgcctcgc ggcgctgggc gtggtgcatg
     4201 cgcgctttgg caccgaggac aagcgcccgg ccgccgaggc catcctggcc gagctgggcc
     4261 tggcctgggg ccaggccgcc gccatgggcg acgactggcc ggatctggcc gtgatgcgcc
     4321 gcgcggcctt tgcctgcgcg ccgccgcagg cccaggccga ggtgcgcgcc atcgcccacc
     4381 atgtcaccgc cgcggccggc ggcgcgggcg ctgcgcgcga gctgtgcgac ctgctgctcg
     4441 tcgccagcgg ccgctatgcc ggcttgctgg ccgcgcacgg cgcatgatga gcagcctgcg
     4501 ccgggcctgg gagcgcctgt ccctctacct gcccgtgctg ctcatggggc tgctggcgct
     4561 gggcacctgg tggctggtgc gcaacgcacc ccagccgcag ccggcggtgg tggagcgccc
     4621 cgtcagccac gagcctgact atttcatgcg cgacttcgcc gtgaaaaact tcgacgccac
     4681 gggccgcttg caaagcgaca tccgcggcca gctggcgcgc cactatgccg acagcgacca
     4741 gttggagatc gaccaggcgc gcatgcgctc ggtcagcccc gaagggcgcg tgaccacggc
     4801 cagcgccaac cgtgcgctga cgaatgccga tggcagcgag gtgcagttgt tcggcaacgc
     4861 catcgtcacg cgcgagcccc tggtgcgcaa aggccaggcc acgttgccgc gcctggagtt
     4921 ccggggcgaa tttctgcatg cctgggtgaa tgatgagcgc gtgcgctcca accagcccgt
     4981 gaccctcaag cgcggctccg acaccttcac cgccgacagc atggactatg acaacctgga
     5041 tcagatcctg aacctgcgcg ggcgcgtgca cggcacgctg cagccggcgg cacgccgctg
     5101 aacccccaga ttggccatgc ccgcgcgccc cctggtcttc atcaccggtg cctccagcgg
     5161 cataggccgg gcgctggccg agcgctacca ccgtgcgggc tggcgactgg ccctggtggc
     5221 ccgccgcgaa cttgagatca aatcatgggc tggcgctcac caattaagcg caagcagcta
     5281 tcaaatttat ggtgctgatg tcgcgcaaat ggacagcatc atcgccgccg gccaggcctg
     5341 cctgcagcac caggggctgc ccgacgtggt catcgccaac gccggcatca gcgtcggcat
     5401 ggacactgcc gagcgcgccg acctggacgt catggcgcgc gtctttgcca ccaacaacct
     5461 gggcctggcc gctaccttcc accccttcat tagcgccatg acacagcgcg gcagcgggcg
     5521 cctggtgggc attggcagcg tggccggcat acgcggcctg cccggccatg gcgcctattg
     5581 cgccagcaag gcggcggtga tcagctactg cgagagcctg cgcggtgaat tgcgcgccag
     5641 cggcgtgcgc gtggtcacga ttagccccgg ctatgtggac acgccgctga cgcgggaaaa
     5701 ccgctaccgc atgccgtttc tgatgacccc cgaagcgttt gccgagagcg ccttcaaggc
     5761 catagaaacc ggccgcagtt accgcgtcat cccctggcag atgggcgtgg ctgccaagct
     5821 gctgcgcctg ctgcccaacc cctggttcga ccgcctgctg cagggacggc cacgcaagca
     5881 ccggcagccg ccttcgagct gaaaaaaacc aagggctccc gagggagccc ttgtgcatgg
     5941 ggtcagtgga tcaataaccg ccgcgatcgc cgccgtagcc gccgccacca ccaccaccgt
     6001 agccgccacg gccaccgccg ccgccaccac cgccgcgcgg gccggagcca taggggctgc
     6061 ggaaaccgcc ttcgccgcgg ccaccgccgc cgccacccgc gccacggcca ccgccgtagc
     6121 cgccgccgcc accaccgccg aaaccaccgc cgaaaccacc gccaccgctg cgcgggggac
     6181 ggggctccat ggggcgcgcc tcgttcacga cgatggcgcg gccacccagg ggctggccgt
     6241 gcatgccgtt gatggcggcc tgggcctctg catcactgcc catttccacg aaaccaaagc
     6301 ctttggagcg accggtgtcg cgttccatca tgaccttggc gctggtcacg gttccgaact
     6361 ggccaaaggc ctgctccaga tcctggtcgc gcaccgagta cggcaggttg ccgacgtaca
     6421 gcttgttgcc catcaaggga ctcctgaaaa acaattgaaa tgagcgacgg agtcccgaac
     6481 ccgcagccag ttgatcacaa agacgcccta tgcgcgaaaa ctgacggatc accgcaacca
     6541 cgatgaaggt cgcccttcac caacggagat tatgttgcaa aactcttggg caaatacaag
     6601 ggggtttgtg cgctcatgtc gccgattggc cgcaaaaaaa ggggccttgc ggccccttgt
     6661 ctgatcgaga tcagtagctg gaacggccac cgccgtagcc gccgcggtcg ccaccgtagc
     6721 cgccgccatt gccgcggcca ccgccgtagc cgccgccacc accgtaacca ccgctgcgcg
     6781 gggggcgggg ctccatgggg cgcgcttcgt tgaccaccag gtcacggccg ccgaaattct
     6841 ggccatgcac gccctggatg gcggcctgtg cctcggcatc gctgcccatc tcgacgaagc
     6901 caaagccctt ggagcggccg gtgtcgcgct ccatcatgac cttggcgctt tgcacggcgc
     6961 cgaactggcc gaaggtttgc tccagatcct ggtcgcggaa ggaatagggc aggttgccca
     7021 cgtaaagttt gttgcccatg acgggactcc tagaaataaa catggaacgc gatggagccc
     7081 gacgcaatca acaaacctgt gacgacttca aagacgcgaa actgaccaga caccgcaaag
     7141 tgctttcctg caggacagga aggctcggcc attatcaagt atccctatgg cctcagaatg
     7201 gtgataagtg cgttccagca acactttcga gggtatttac cgggtatttg ttgtccatcg
     7261 ggcggcaccg gcgcggatgg atacaatgcc ggccgtccat ggggagtagc ctgcccacgc
     7321 gcgccgcagg cgccacggga ggcatgcatc aacagacttg gcgcgcccgc gcgccatggt
     7381 gcatgaggtt cgccgcaatg gcggactggg cgagaccatt gaccatgcac cgatccatca
     7441 ggccggagtc ggggcgtggt caatgctgtc cgaatgcttc cggccggaaa tcccttgacc
     7501 cagtcccgct atggaagcct ttctcatctc cacctccatc gtcgccctcg cagaaatggg
     7561 cgacaagacc cagctgctgt ccctggtgct ggcggcgcgt tttcgcaagc cctggcccat
     7621 cgtgctgggc attctggtgg ccacgctggc caatcatgcg ctggccgggg ccgtcggcgc
     7681 gtgggtgacc acggtactgg ggccgcaggc gctgcgctgg gtgctgggcc tatcgttcat
     7741 cgccatggcg gtgtggatgc tgatccccga caagctcgac gatgacggcg agggcaaggg
     7801 cgtgcgcctg ggcgtgttcg gcaccaccgt ggtggccttc ttcatcgccg agatgggtga
     7861 caagacgcag attgccaccg tcatgctggc ggcgcagtac aacgcctatc tgtgggtggt
     7921 ggccggcacc acgctgggca tgatgattgc caacgcaccc gtggtctggc tgggtgaccg
     7981 cctggtcaag aaggtaccga tacgcctggt gcacatcatc tcggccgtga tttttctggt
     8041 gctgggtctg ctggcgctgt ttgtgccgca ggccacgcct ttggcataat gcccgccaga
     8101 cgcgccgatt tgctaccgct tgcgggcgct ttataaattc agctaaagac atgaccgatg
     8161 gatgcatgac ccggcctcgt ttttagcgac agccgggttt tttcatgtcg ttcatcgcca
     8221 caccgcaatc catgcacttt gccgaggccc tgcgcttgca gtcgggcgcc tcgatacgcg
     8281 actacaccct ggcctacgag acctacggcg agctcaacgc cgcgcgtgac aacgccgtgc
     8341 tggtgtgcca cgcgctcaac gcctcgcacc atgtggcggg cgtctatgag ggccagccca
     8401 agagcgaggg ctggtgggac aacatgatcg gccctggcaa gccgctagat accaaccgtt
     8461 tcttcgtcat cggcatcaac aacctgggct cctgctttgg ctccaccggg cccatgcacg
     8521 cgcacccgga cacgggcgag gtctatggcg ccgacttccc cgtggtgacg gtggaggact
     8581 gggtcaacgc ccaggcgctg ctgctcgatc gcctgggcgt tgcgcaactg gccgcggtga
     8641 tgggcggcag cctgggcggc atgcaggcgc tgtcgtggac gctgcaatac ccggagcgtg
     8701 tgcgccacgc cgtggtggtg gccagcgcgc ccaatctgac ggccgagaac atcgccttca
     8761 acgaggtggc gcgccgcgcc atcgtcaccg acccggactt tcacggcggg catttctacc
     8821 gccatggcgt gattccgcag cgcggcctgc gcatcgcgcg catgatcggc cacatcacct
     8881 acctgagcga cgacgtgatg aacgagaagt tcggccgtca gctcagggag ggcatggatc
     8941 tcaagtacag cacccaggac atagagttcc agatcgagag ctacctgcgc taccagggcg
     9001 acaagttcag cgagtatttc gatgccaaca cctatctgct catcacgcgc gcgctggact
     9061 acttcgaccc cgcgcgtcgg catggcggca acctggccca ggcgctggcc gtggcgcgtg
     9121 ccaaatttct gctggtgagc ttcagcaccg actggcgctt ttcgccccag cgcagccgag
     9181 aaatcgtgca ggcgctgctg gagaaccgcc gcgacgtgag ctacgccgag atcgacgctc
     9241 cccatggtca tgatgcattt ttgctggatg acgcccgata catgggcgtg gtgcgctctt
     9301 attttgatag tatttccaag gagctgcagg gaggacaacc atgagcgacc acgccaccat
     9361 gcaagccatt gcacgcctgg tgccagaagg cagccgcgtg ctcgacttag ggtgcggcga
     9421 cggcgccctg ctcgacctgc tgcagcgcga acgcggctgc agcggctacg gcatagagct
     9481 ggccgacgcc aatgtgctgg cctgcgtgcg ccgcggcgtc aacgtcatcc agctgaacct
     9541 ggacgaaggc ctggccatgt ttggcgacaa cagcttcgac gtggtgctgc agatcgacac
     9601 cctgcagcat ctgcgcaatg ccgaggtcat gttgcgcgag acggcgcgcg tgggccaaag
     9661 cggcatcgtc gcctttccca actttgcgca ctggccgaac cgcctgtcgg tgctgcgcgg
     9721 acgcatgccg gtcacgcggc gcctgcccta ccagtggtat gacacgccca acatccgcgt
     9781 cggcacctac aaggacttcg aggtgctggc gtacaagaac aagctgcgca tcctggacgc
     9841 cttcggcctg caggacgggc gcgaggtgcg ctggctgccc aatgcactgg cgggcacggc
     9901 ggtgttccgc ttcgagcacg actgacaaac cccgtggcgc agaccctggc cttggggtaa
     9961 aaaggccgtc tcacatcttc ttgctgcccc atgctgcccc ttgatgtgcc caccatgctg
    10021 ctgatgacgg cggcagcctc cgccaccatg gcgctgtcca tggccgccgt tcggcccgag
    10081 cggcgcgaag gcatgggcct gtgggcactg gcgctggtgc tgcacaccat tacctatctg
    10141 ctgttcacgc tgcgcggcgc ggtgcccgcc tgggccagtg tggtgctggc caataccctg
    10201 ctggccggca cctttgcgct ggcgctggcg gccgtgcgcc agtttcaggg ccatgggctg
    10261 ccctggcgcc gcatgctggt gccggtgctg gccatggcgc tgctgtttgc ctggttccag
    10321 gacgactacc gcgcctgcgt gatcgtcgcc ggcgtggtgc cgccgctgca ggtggccctg
    10381 gtgctgtggg cgctgtggcg gcccaagccg ccggcgcaaa tgcgtggcgc cgtcttgctg
    10441 accgcggggc tgggcctgca ggccgtgctg ctggcggtgc gcggtgcgct ggccgccctg
    10501 cacagcatcc ccacccaagg cctgctgcgc accgatggcg tgcagtcgct gaccttcatg
    10561 gcggcctttg tcgtggtcat cctggcgtcg ctgggcttca tcctgatggc caaggacagg
    10621 gccgacgcca acaaccatta ctttgccacc cgcgacgccc tgaccggcct gttcaaccgc
    10681 cgcgccctgc tgctggcggc gcgccgtgac gtggctgttg ccgcacgctc gcatgaaccc
    10741 tatgcgctga tgatggtgga catagaccat ttcaaggccg tcaacgacgg ccatggccac
    10801 caggtgggcg accagatcct gtgccatgtg gcgggcctgc tcagcgccag gctgcgcgcc
    10861 caggacatgg tggggcgcta cggtggcgag gaattcctgg tgctgctgcc caacaccgcg
    10921 cagcaggccg ccgccgagct ggccgaaacc ctgcgcagcg ccgtggccca gtgcccctgt
    10981 gcgcgcgagg gctgcgccat caccgccacg gtgagcattg gcgtgtgtgg cggccagctg
    11041 catggcaccg aggactggga gcgactgctg cgctgcgccg accaggcgct gtatgccgcc
    11101 aaggccgccg ggcgcaaccg
//
LOCUS       DAOMTQ010000102        10939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1138_length_10939_cov_28.019202, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000102 DAOMTQ010000000
VERSION     DAOMTQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1138_length_10939_cov_28.019202"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /gene="flgE"
                     /locus_tag="PK518_10475"
     CDS             complement(<1..1199)
                     /gene="flgE"
                     /locus_tag="PK518_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRM49383.1"
                     /translation="MGFQQGLSGLNASSKNLDVIGHNIANSGTVGFKASRTEFAAMVA
                     NAIGTAGSGNAGIGVQVGAVAQQFTQGNISVTGNNLDVAINGNGFFRIQQPDGSTAYT
                     RAGNFKLDDKGNVVTNNHGKVMGYPMDPVTRTRTASTPQPLSFPTGVPIPAKESTTIK
                     ASFNLDARAPDAAGNAAATPPVAPTPRSTYGTSINVYDSQGVATPVSLYFQKNGANTW
                     DVYDKLDDPNATPPIVATPIGQIQFDNNGNIIGPAATPPATGFQMPVTIAPPTPNPNN
                     LPAYTVQVNLDGVTQFGTKFAVSELTQDGYTAGQLTGINIGNDGTIMASYSNGVTRAE
                     GQVALASFRNTQGLADIGNNLWQETFESGQPVMGVPTEGAFGALRSGALEDSNVDLTA
                     ELVNMMTA"
     gene            complement(1241..1903)
                     /locus_tag="PK518_10480"
     CDS             complement(1241..1903)
                     /locus_tag="PK518_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRM49384.1"
                     /translation="MNISPIDTSALDNGANTTSKTRDSSTDPSAMQDRFLKLLVAQLN
                     NQDPMNPLDNAQMTTQMAQINTVTGLQTLNLTMQTMAEQFSTMQQIQGISLIGRSVLS
                     EGDRMSLDDKTGKGMFDLAGAASSVKVEIVTPGGVAVDTIDMGAQEGGRLNFEWDASK
                     YQGNAAELRFRVTAANGETAVKATPLTQSQVLATGSKSGALTLTLADGSTVNYANILG
                     VL"
     gene            complement(1920..2324)
                     /gene="flgC"
                     /locus_tag="PK518_10485"
     CDS             complement(1920..2324)
                     /gene="flgC"
                     /locus_tag="PK518_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRM49385.1"
                     /translation="MSMFSIFNVSGSAISAQSQRLNVVASNLANVDAVAGPDGQAYKA
                     RQVVFQTTPMGSDGAAGVRVTGIQESDTPGRRVHDPMNPMADAEGYVTHANVNAVEEM
                     VNMISASRSYQNNVEVMNTAKSLLLKTLQMGQ"
     gene            complement(2336..2752)
                     /gene="flgB"
                     /locus_tag="PK518_10490"
     CDS             complement(2336..2752)
                     /gene="flgB"
                     /locus_tag="PK518_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRM49386.1"
                     /translation="MLDKLTQRLDFHGNALLLRAERQRTIASNIANADTPGFVARDFK
                     FADAMRAVDEGRSPGTLTRQSVSDARHIPLPAARTGSGDSTLGYAVQTQPSLDNNSVD
                     MDRERAAFVDNAVRYEATLRFINGNAKTMLSAIQGQ"
     gene            2961..3695
                     /gene="flgA"
                     /locus_tag="PK518_10495"
     CDS             2961..3695
                     /gene="flgA"
                     /locus_tag="PK518_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRM49387.1"
                     /translation="MPHSPFRRQRAATWLAWACLAGLAALAVPARAQEQTPELGAITQ
                     RWLDAALAQDQGALPLRMEVSVGQLDPRLRLAPCARVEPHLPAGARLWGRTRLGLRCV
                     EGAVRWNVYLPITVKAFGPAWVLAGGVAAGAVLKEGDAIESEVDWAAESAAIVARPEA
                     WVGLIAARPLQAGQALRQNMVRAPDLFQSGASVRVVVQGQGYAVTASGQALSAGAQGQ
                     NVRVRMSNGRFVSGIVQEDGTIEATL"
     gene            3789..4112
                     /gene="flgM"
                     /locus_tag="PK518_10500"
     CDS             3789..4112
                     /gene="flgM"
                     /locus_tag="PK518_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRM49388.1"
                     /translation="MKIGNNPDTANLLSQAAAARQQAKAAAPAPAAEAAAQGASRTAA
                     GVPVTLSATVRGADASRSSADFDAGKVKAVKAAIENGTFKVNAEAVADKLLANAEETL
                     SRSRG"
     gene            4155..4493
                     /locus_tag="PK518_10505"
     CDS             4155..4493
                     /locus_tag="PK518_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49389.1"
                     /translation="MSIDNTLSLIEAQLQDMQAALLSSDPQALEQTAAQLRQVATSLA
                     QAAGAGLPAAAAERIQSIATRLTLVRDQLARVLALTERQAASLLPPVQGVTYGPSSGA
                     QGARIYRAPG"
     gene            complement(4527..5357)
                     /gene="phhA"
                     /locus_tag="PK518_10510"
     CDS             complement(4527..5357)
                     /gene="phhA"
                     /locus_tag="PK518_10510"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HRM49390.1"
                     /translation="MAVQPAVYGASERPPRGDYTRAADDYTCAQNWAAYTAQDHDTYH
                     RLYLRQCEQLPGLASAAFIAALPQLGAKERIPRFDDINERLYKATRWQLVAVPGLIPE
                     VPFFQLLANRKFPVTDWIRRPEEFEYIVEPDVFHDLFGHVPLLFNPMLADYMQRYGEG
                     GLKAARLSSCEMLARLYWYTIEFGLIREAEGLRAYGAGILSSHGELPYSVHSPEPQRL
                     PLTLERTMRTRYKIDSYQQTYFVIDSFEQLFQLTEGDFTPLYERLRGLPEFAADERNP
                     "
     gene            complement(5359..6486)
                     /gene="hppD"
                     /locus_tag="PK518_10515"
     CDS             complement(5359..6486)
                     /gene="hppD"
                     /locus_tag="PK518_10515"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRM49391.1"
                     /translation="MTAAPPSQATRDTTAWENPMGLMGFEFVEFTSPQPGVLEAVFEK
                     LGFTLVAKHRSKDVLLYRQNQINFILNREPHSQAAYFGAEHGPSACGLAFRVKDAHKA
                     YKRALELGAQPIEIPTGPMELRLPAIKGIGGAPLYLIDRFEDGKSIYDIDFEWLPGVE
                     QRPAGHGLNEIDHLTHNVYRGRMEFWSKFYESLFNFRELRYFDIQGEYTGLTSKAMTA
                     PDGKIRIPLNEEARQGGGQIEEFLMQFNGEGIQHIALICDSLVEVVDKLGLAGVPLAP
                     APNDVYYQMLEKRLPGHGQNVNDLQARGILLDGTTADGTPRLLLQIFSTPMLGPVFFE
                     FIERRGDYREGFGEGNFKALFESLERDQVNRGVIDTGAKEA"
     gene            6650..7135
                     /locus_tag="PK518_10520"
     CDS             6650..7135
                     /locus_tag="PK518_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM49392.1"
                     /translation="MTERTVALDKLDRAILRRLQDNGRETYDLIGEQVGLSPSAVLRR
                     AKRLEEEGVIDRYVALVRPERVGLGLTAYLNVRLEKHTESHKRNPMDLFRASVQTWPE
                     VVECAALTGEMDYLLRVVVQDMAHYSRFIMDTLLKHPSVQDCKTSFVLDRVKATTAVP
                     V"
     gene            7285..8007
                     /locus_tag="PK518_10525"
     CDS             7285..8007
                     /locus_tag="PK518_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM49393.1"
                     /translation="MLSPHHWLRTTLLAGRHMLLPAGRRAHRGFAMNSIVPIRALGAQ
                     HRERVANHLLALTERDRYLRFGYSASDEQVRRYAELLDFERDEVFGIFNRRLQLIAMA
                     HVAYAQSEDTQSCAEFGVSVLASARGRGYGGLLFDRAVTLARNRGVRMMFIHALTENT
                     AMLKIARNAGARVHQDGSESQAYLQLPPAGLDTRMSKIVAEHFGEVDYQLKKQARQFW
                     SLLADMQEIRQGVQDGRHQAAE"
     gene            8092..8973
                     /locus_tag="PK518_10530"
     CDS             8092..8973
                     /locus_tag="PK518_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRM49394.1"
                     /translation="MSDPYPARPPARQDKRTLLQRLVEFISPGPDSTAELIATLADAE
                     DNQVINTDERVMLERVLRMAEMTAADVLVPAPRMDMLDIDAPLDALMYQVLRTAHSRF
                     PVYQGEREHIIGILLAKDLLKLWRSPDLNIRTLVRPALFVPESKGLQALEREFRSTRN
                     HMAIVIDEFGRIAGLVTFEDVIEQIVGEIEDEFDIHEDEGDIFGLPDHSYRVSGETPV
                     ERVAEAFEVTLRSSDPDEVFDTIGGLIAHELGRVPRKGEVLLLGGLRFVVLHTKGGLV
                     RWFKVTPAAADESATNS"
     gene            8973..10562
                     /gene="lnt"
                     /locus_tag="PK518_10535"
     CDS             8973..10562
                     /gene="lnt"
                     /locus_tag="PK518_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRM49395.1"
                     /translation="MTRALFLEAPATSRGPLPWLLALGAGLGQAASLAWPGPGSLAGQ
                     PLWWLQILSLALLGRVLLGAGSARRAALLGWLFATSWLVGTFWWLFISMHTYGGLAAP
                     LAALAVLALALFLASYYAAVSALFWRVAPNCRAQAAMIFAALWLLAELARGQWWTGFP
                     WGAGGYAHADGPLAVLARSVGVYGMGLVAAALAMGLAQWRPGDLRRARWWAGLALLLL
                     AWAGLAWQRHCAVDGCGAAAEEHKSLSVTLLQGNIPQDEKFQPGSGVPLALHWYYEQV
                     RFTDAALVVAPETAIPLLPQQLVPGYLEEIAERFSSGRQALLLGIPLGNLEQGYTNSV
                     LGFAPGQQPPYRYDKHHLVPFGEFIPPFFRWFTEMMNIPLGDFNRGAVGQPSLPWAGE
                     RIAPNICYEDLFGEELGARFRDPASAPTIMANMSNIGWFGDTVAIDQHLAISRLRALE
                     FERPMLRATNTGATAVIDHRARVTHRLPAFQRKALNAQVQGRGGAVTPYAWWVSRWGL
                     APLWALGLGLAAWAWLRRRRH"
     gene            10680..>10939
                     /gene="fabA"
                     /locus_tag="PK518_10540"
     CDS             10680..>10939
                     /gene="fabA"
                     /locus_tag="PK518_10540"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HRM49396.1"
                     /translation="MADSFSYEQLIASGEGRLFNPDSGRLPLPPMLMFDRITHIDADG
                     GAHGLGKIVAELDVKPDLWFFDCHFQGDPVMPGCLGLDAMWQL"
BASE COUNT         1614 a         3513 c         3817 g         1995 t
ORIGIN      
        1 gcggtcatca tgttcaccag ctcggcggtc agatcgacgt tggagtcctc cagcgcgccc
       61 gagcgcagcg cgccaaaggc gccctcggtg gggactccca tgacgggctg gccggactcg
      121 aaggtctcct gccagaggtt gttgccgatg tcggccaggc cctgggtgtt gcgaaagctg
      181 gccagcgcca cctggccctc ggcgcgcgtc acgccgttgg agtagctggc catgatggtg
      241 ccgtcgttgc cgatattgat gccggtgagc tggccggcgg tgtagccgtc ctgcgtgagc
      301 tcggagacgg cgaacttggt gccgaactgg gtcacgccgt ccaggttgac ttgcaccgtg
      361 taggccggca ggttgttcgg gtttggggtg ggtggcgcga tggtcaccgg catctgaaaa
      421 cccgtggcgg gtggcgtggc ggcggggccg atgatgttgc cgttgttgtc gaactggatc
      481 tggccaatgg gcgtggccac gatgggcggc gtggcgttcg ggtcgtcgag cttgtcgtag
      541 acgtcccagg tgttggcgcc gttcttctgg aaatacaggc tcaccggcgt ggccacgccc
      601 tggctgtcat agacattgat cgaggtgcca taggtcgagc gcggcgtcgg cgccaccggt
      661 ggcgtggccg cggcattgcc cgcggcgtcg ggcgcgcgcg cgtccaggtt gaacgaggcc
      721 ttgatggtcg tgctctcctt ggccgggatg ggtacgcccg tcggaaagga cagtggctgc
      781 ggcgtgctgg cggtgcgggt tctggtgacg ggatccatgg ggtagcccat gaccttgccg
      841 tggttgttgg tgaccacgtt gcccttgtcg tccagcttga agttgccggc gcgcgtatag
      901 gccgtgctgc cgtcgggctg ctggattctg aagaagccat tgccgttgat ggccacgtcc
      961 aggttgttgc cggtcaccga gatattgccc tgcgtgaact gctgggccac ggcgcccacc
     1021 tgcacgccta tgccggcatt gcccgagccc gccgtgccta tggcattggc caccatggcg
     1081 gcaaactcgg tgcgcgaggc cttgaagccc acggtgcccg agttggcgat gttgtggccg
     1141 atcacgtcca ggttcttgct ggaggcgttg aggcccgaga ggccttgctg gaaacccatg
     1201 gtgtgctcct tgtcggtgag gggtggctgg cgtgatggcg ttagagcacg ccgaggatgt
     1261 tggcgtagtt gacggtgctg ccgtcggcga gggtcagggt cagcgcgccg gacttggagc
     1321 cggtggccag cacctgcgac tgggtcagcg gcgtggcctt gaccgcggtc tcgccattgg
     1381 cggcggtgac gcgaaagcgc agctcggccg cgttgccctg gtatttgctg gcgtcccatt
     1441 caaagttcag ccgcccgccc tcctgcgcgc ccatgtctat ggtgtccacg gccacgccgc
     1501 cgggcgtgac gatctccacc ttgacgctgc tggcggcgcc ggccaggtcg aacatgccct
     1561 tgccggtctt gtcgtccagg ctcatgcggt cgccctcgga cagcaccgag cggccgatca
     1621 gcgaaatgcc ctggatctgc tgcatggtgc tgaactgctc ggccatggtc tgcatggtca
     1681 ggttcagcgt ctgcagaccg gtgacggtgt tgatctgcgc catctgcgtg gtcatctgcg
     1741 cgttgtccag cgggttcatc gggtcctggt tgttcagctg ggcgacgagc agcttcagga
     1801 agcggtcctg catggcgctc gggtcggtcg atgagtcgcg cgtcttggac gtggtgttgg
     1861 cgccgttgtc cagcgcgctg gtgtcgatgg gggaaatgtt catgcttgtc ctcccggcct
     1921 tactggccca tctgcagggt cttgagcagc agcgacttgg ccgtgttcat gacctcgacg
     1981 ttgttctggt acgagcgcga ggccgagatc atgttcacca tctcctccac cgcgttcacg
     2041 ttggcgtgcg tcacatagcc ctcggcgtcg gccatggggt tcatggggtc atgcacgcgc
     2101 cggccggggg tgtcgctctc ctggataccg gtgacgcgca cgccggcggc gccgtccgat
     2161 cccatgggcg tggtctggaa caccacctgg cgcgccttgt aggcctggcc gtcggggccg
     2221 gccacggcgt ccacgttggc caggttggag gcgacgacgt tgagccgctg cgactgcgcg
     2281 ctgatggcgc tgcccgagac gttgaagatg gaaaacatcg acatgcgaag gctccttatt
     2341 gaccctggat ggcgctgagc atggtcttgg cgttgccgtt gatgaagcgc agcgtggcct
     2401 cgtagcgcac ggcgttgtcc acgaaggcgg cgcgctctct atccatgtcc acgctgttgt
     2461 tgtccaggct gggctgggtc tgcacggcat agcccagcgt gctgtcgccg ctgcccgtgc
     2521 gcgcggcggg cagcgggata tggcgcgcat cgctcacgct ctggcgcgtg agcgtgccgg
     2581 ggctgcggcc ctcgtccacg gcgcgcatgg cgtcggcaaa cttgaagtcg cgcgccacaa
     2641 agcccggcgt gtcggcattg gcgatgttgc tggcaatggt gcgctggcgc tcggcacgca
     2701 gcagcagcgc gttgccgtga aagtccagtc gctgggtcag cttgtcgagc atgggggcct
     2761 gcagtccttg tggttcggtg cttgcggcgc cacggcccgg gggcttttgt ggcgtgcgtc
     2821 gattatggaa atcgccccgc ttttctaaag cccgaagaac gcgggcattg gccctttgtt
     2881 tgcgtgttat cgcggtgggg tcgcgcctac agtgacggct gagaaacggc gcctgtcgcg
     2941 tcgtttgccg gagtcacgcc atgccgcatt caccattccg ccgacaacgt gccgccacct
     3001 ggttggcctg ggcttgcctg gctggcctgg cggcgctggc cgtgccggcg cgtgcccagg
     3061 agcagacgcc cgagctgggc gccatcaccc agcgctggct ggatgcggcc ctggcccagg
     3121 atcagggcgc attgccgctg cgcatggagg tcagcgtagg gcagctcgat ccgcgcctgc
     3181 gcctggcgcc ctgcgcgcgt gtcgagccgc atctgcccgc cggcgcccgc ctgtgggggc
     3241 gcacccggct ggggctgcgc tgcgtggagg gggcggtgcg ctggaatgtg tatctgccca
     3301 tcaccgtcaa ggccttcggc ccggcctggg tgctggcggg cggcgtggct gcgggcgctg
     3361 tgctcaagga gggtgacgcc atcgagtccg aggtggattg ggccgccgag agcgccgcca
     3421 tcgtcgccag gcccgaagcc tgggtgggtc tgattgcggc gcggccgctg caggcgggcc
     3481 aggcgctgcg ccagaacatg gtgcgcgcgc ccgacctgtt tcagtccggc gcctcggtgc
     3541 gcgtggtggt gcaggggcag ggctatgccg tgacagcctc gggccaggcg ctgagcgccg
     3601 gcgcgcaggg gcagaatgtc cgtgtacgca tgagtaatgg ccgctttgta tcgggcattg
     3661 ttcaggaaga tgggacaata gaggccactc tgtgaaccat tgagccgcac aaagcgctaa
     3721 agtccgccca ccactggccg aaaacatggc tactggcccc gcagcagatc ggggtagtgg
     3781 agagtgtgat gaaaatagga aacaacccgg acaccgcgaa tctcttgtcg caggccgcag
     3841 ccgccaggca gcaggccaag gcggccgcac ccgcgccggc cgccgaggcc gccgcccagg
     3901 gtgcgtcgcg caccgcggcg ggggttcccg tcacgctgtc ggccacggtg cgtggcgcgg
     3961 atgccagccg cagcagcgcc gacttcgacg ccggcaaggt caaggccgtg aaggcggcca
     4021 tagagaacgg cactttcaag gtcaacgccg aggccgtcgc cgacaagctg ctggccaacg
     4081 ccgaggaaac cctgtcgcgc tcgcgcggct gagtgctgcc tgcctgacgg ggtggccggc
     4141 tctggacttg ccccatgtct atcgacaaca ccctttccct tatcgaagcg cagctgcagg
     4201 acatgcaggc tgcgcttttg tcttccgacc cccaggctct ggagcaaacg gccgcgcaac
     4261 tgcgccaggt ggccaccagc ctggcgcagg ccgcaggtgc cggcctgcct gccgctgctg
     4321 ccgagcgcat ccagtccatc gccacgcgcc tgaccctggt gcgcgaccag ctggcccgcg
     4381 tgctggcgct gaccgagcgg caggcggcgt cgctgctgcc ccccgtgcag ggcgtgacct
     4441 atgggccatc ttccggcgcg cagggtgcgc gcatctatcg ggcgccgggg tagtctccgg
     4501 ttgggttaat tggaatctga ccccaattat gggttgcgct cgtcggccgc gaactcgggc
     4561 aggccgcgca ggcgttcgta cagtggcgtg aagtcgccct cggtgagctg gaacagctgc
     4621 tcgaaactgt cgatgacgaa gtaggtctgc tggtagctgt cgatcttgta gcgcgtgcgc
     4681 atggtgcgct ccaatgtcag cggcaggcgc tggggctcgg ggctgtgcac gctgtagggc
     4741 agctcaccgt gcgagctcaa aatgcccgcg ccataggcgc gcaggccctc ggcctcgcgg
     4801 atcaggccga actcgatggt gtaccagtac agccgcgcca gcatctcgca ggagctcagg
     4861 cgcgcggcct tcaggccgcc ctcgccgtag cgctgcatgt agtcggccag catggggttg
     4921 aacaagagcg gcacatggcc gaacaggtcg tggaacacgt cgggctcgac gatgtactca
     4981 aactcttcgg gccggcgtat ccagtcggtc accgggaact tgcgattcgc cagcagctgg
     5041 aagaacggca cctcggggat cagccccggc acggccacca gctgccagcg cgtggccttg
     5101 tacaggcgct cgttgatgtc atcgaagcgc ggaatgcgct ccttggcgcc cagctggggc
     5161 agggcggcga tgaaggccgc gctggccagg ccgggcagct gctcgcattg ccgcaggtac
     5221 aggcggtggt aggtgtcgtg gtcctgcgcg gtgtaggcgg cccagttttg ggcgcaggtg
     5281 tagtcgtcgg cggcgcgcgt gtagtcgccg cgaggcgggc gctcgctggc gccatagacg
     5341 gcgggttgca cggccatgtc aggcttcctt ggcgccggtg tcgatcacgc cgcggttcac
     5401 ctggtcgcgc tccagcgact cgaaaagtgc cttgaagttg ccctcgccaa agccctcgcg
     5461 gtagtcgccg cggcgctcga tgaactcgaa gaacaccggg cccagcatgg gcgtggagaa
     5521 gatctgcagc aagaggcgcg gcgtgccgtc ggccgtggtg ccgtccagca gaatgccgcg
     5581 cgcctgcagg tcgttcacgt tctggccatg gcccggcagg cgcttttcca gcatctggta
     5641 gtacacatcg ttgggtgcgg gcgccagcgg tacgccggcc aggcccagct tgtccaccac
     5701 ctcgaccagc gagtcgcaga tcagcgcgat gtgctgtatg ccctcgccgt tgaactgcat
     5761 caaaaactcc tcgatctggc cgccgccctg gcgcgcctcc tcgttcagcg ggatgcggat
     5821 cttgccgtcg ggcgccgtca tggccttgga cgtcaggccg gtgtactcgc cctggatgtc
     5881 gaagtagcgc agctcgcgga agttgaacag gctctcgtag aacttggacc agaactccat
     5941 gcggccgcgg tagacgttgt gcgtcaggtg gtcgatctcg ttgaggccat ggcccgctgg
     6001 gcgctgctcg acgccgggca gccattcgaa gtcgatgtcg tagatggact tgccgtcctc
     6061 gaagcggtcg atcaggtaca gcggcgcgcc gcctatgccc ttgatggccg gcaggcgcag
     6121 ctccatgggg ccggtgggaa tctctatggg ctgggcgccg agctccagcg cgcgcttgta
     6181 ggccttgtgc gcgtctttca cgcggaaggc caggccgcag gccgatgggc catgctcggc
     6241 gccaaagtag gccgcctggc tgtgcggttc acggttcagg atgaagttga tctggttctg
     6301 gcggtacagc agcacgtcct tggagcggtg ttttgccacc agggtgaagc cgagcttctc
     6361 gaacaccgcc tccagcacgc cgggctgggg cgaggtgaac tcgacgaact cgaagcccat
     6421 caggcccatg gggttctccc aggcagtggt gtcgcgggtg gcttggctgg gcggggctgc
     6481 ggtcatgggg ttttgtctcc gatgattggt gtaaacatgc cactgtaggc gtctgctggc
     6541 tcatgtttct ggcgaatgca tcctggttcg attctcaatg tgcaggaatg ttgcgcaaca
     6601 tcattgcaat gtgcagctat ttggcgttgg ctgattaaaa tcccttgcca tgaccgaacg
     6661 caccgttgcc ctggacaagc tcgaccgcgc catcctgcgc cgcctgcagg acaacggccg
     6721 cgagacctac gacctgatcg gcgagcaggt gggcctgtca cccagcgccg tgctgcgccg
     6781 cgccaaaagg ctggaggagg agggcgtgat cgaccgctac gtggccctgg tgcgccccga
     6841 gcgcgtgggg ctgggcctga ccgcctacct gaacgtgcgc ctggaaaagc acaccgagag
     6901 ccacaagcgc aaccccatgg atctgtttcg cgccagcgtg cagacctggc ccgaggtggt
     6961 ggaatgcgct gccctgaccg gcgagatgga ctacctgctg cgcgtggtgg tgcaggatat
     7021 ggcgcattac agccgcttca tcatggacac cctgctcaag caccccagcg tacaggactg
     7081 caaaaccagc ttcgtgctcg accgcgtcaa ggccaccacc gccgtgccgg tctgaatgta
     7141 tactatcatt aatatagctt gtagcgcttt ttggacaagc gctacggcca tatttgatgc
     7201 tggttgcaaa accccgacaa agtatgctgc aatgcaacat gtgtatgcgc gagaactagg
     7261 gaaaacccta gaattcacag catcatgctt tctccccacc attggctgcg cacaaccctt
     7321 cttgccggcc gccacatgct gctgcccgcc gggcggcgtg ctcaccgagg ctttgccatg
     7381 aacagcatcg tccccatacg cgccctgggc gcacagcatc gcgaacgcgt cgccaaccat
     7441 ctgctggcgc tgaccgagcg cgaccgttac ctgcgctttg gctacagcgc cagcgacgag
     7501 caggtgcgcc gctacgcgga gctgctggac ttcgagcgcg acgaggtgtt cggcatcttc
     7561 aaccgccgcc tgcagctcat cgccatggcc catgtggcct atgcccagtc cgaggacacg
     7621 caaagctgcg ccgagtttgg cgtctcggtg ctggccagcg cgcgcgggcg cggctacggc
     7681 gggctgctgt tcgaccgcgc cgtgaccctg gcgcgcaacc gcggcgtgcg catgatgttc
     7741 atccacgcgc tgaccgagaa cacggccatg ctgaagatcg cgcgcaacgc cggcgcccgc
     7801 gtgcaccagg acggctccga gtcccaggcc tatctgcagc tgccgcccgc ggggctggac
     7861 acgcgcatgt ccaagatcgt ggccgagcat ttcggcgagg tcgactatca gctcaagaag
     7921 caggcgcgcc agttctggag cctgttggcc gacatgcagg aaatccgcca gggcgtgcag
     7981 gacgggcgcc accaggcggc tgagtagcac cgcgcggcgg atcgcggccg gggcgatccg
     8041 ctatccttgg gcccctgtcc caccactttg cacgccctgc acccgccgcc cgtgtccgac
     8101 ccctatcctg cgcgcccgcc tgccaggcaa gacaagcgca cgctgctgca gcgcctcgtc
     8161 gagttcatca gtcccgggcc ggactccacc gccgagctca tcgccacgct ggccgatgcc
     8221 gaggacaacc aggtcatcaa caccgatgag cgcgtgatgc tcgagcgcgt gctgcgcatg
     8281 gccgagatga cggccgccga cgtgctggtg cccgccccgc gcatggacat gctggacatc
     8341 gacgcgccgc tggatgcgct gatgtaccag gtgctgcgca cggcgcattc gcgctttccg
     8401 gtgtaccagg gagagcgcga gcacatcatc ggcatcctgc tggccaagga cctgctcaag
     8461 ctctggcgct cgcccgacct gaatatccgc accctggtgc gcccggcgct gttcgtgccc
     8521 gagagcaaag gcctgcaggc gctggagcgc gagttccgca gcacgcgcaa ccacatggcc
     8581 atcgtcatcg acgagttcgg ccgcattgcc ggcctggtca ccttcgagga tgtgatcgag
     8641 cagatcgtcg gcgagatcga ggacgagttc gacatccacg aggacgaggg cgacatcttc
     8701 ggcctgcccg accacagcta ccgcgtgagc ggcgaaaccc ccgtggagcg cgtggccgag
     8761 gccttcgagg tgacgctgcg ctccagcgac ccggatgagg ttttcgacac cataggcggg
     8821 ctgatcgcgc atgagctcgg ccgcgtgccg cgcaagggcg aggtgctgct gctgggcggc
     8881 ctgcgcttcg tggtgctgca caccaagggc ggcctggtgc gctggttcaa ggtcacgccg
     8941 gctgcggcag acgagagcgc gactaactcc tgatgacgcg cgccttgttt ttggaggccc
     9001 cggccacaag ccgcgggccg ctgccctggt tgctggcgtt gggcgccggc ctcgggcagg
     9061 ccgcgtcgct ggcctggcct gggccgggta gcctggccgg gcagccgctg tggtggctgc
     9121 agatcctgtc gctggcgctg ctggggcgcg tgctgcttgg cgccggctcg gcgcggcgtg
     9181 cggccctgct gggctggctg ttcgccacca gctggctggt tggcaccttc tggtggctgt
     9241 tcatctccat gcacacctat ggcggcctgg ccgcgccgct ggcggcgctg gcggtgctgg
     9301 cgctggcgct ctttctggcc agttactatg cggcagtttc tgcattgttt tggcgtgtgg
     9361 cgcccaactg tagggcgcag gcagctatga tttttgctgc gttgtggctg ctggccgagc
     9421 tggcgcgcgg ccagtggtgg accggctttc cctggggcgc gggtggttac gcccatgccg
     9481 atgggccgct ggccgtgctg gcgcgcagcg tcggcgtgta cggcatgggg ttggtggcgg
     9541 ccgccctggc catgggcctg gcgcagtggc gtccgggcga tctgcgccgg gctcgctggt
     9601 gggccggcct ggccctgctg ttgctcgcct gggcgggcct ggcctggcag cgccattgcg
     9661 ccgtcgacgg ctgcggcgcg gcggcagagg agcacaaaag cctgtccgtc acgctgttgc
     9721 agggcaatat cccgcaggac gagaagttcc agcccggcag cggcgtaccc ctggccctgc
     9781 actggtacta cgagcaggtg cgctttaccg atgccgccct ggtggtggcg cccgagacgg
     9841 cgatcccgct gctgccccag caactggtgc cgggctacct ggaggagatt gccgagcgct
     9901 tctccagcgg tcgccaggcg ctgctgctgg gcataccgct gggcaacctg gagcagggct
     9961 acaccaactc ggtgctcggc ttcgcgccgg gccagcagcc gccctaccgc tacgacaagc
    10021 accacctggt gccgtttggc gagttcatac cgccgttctt ccgctggttc accgagatga
    10081 tgaacatccc gctgggcgac ttcaaccgcg gcgccgtggg ccagccctcg ctaccctggg
    10141 cgggcgagcg catcgcgccc aacatctgct acgaggattt gtttggcgag gagctgggcg
    10201 cgcgctttcg cgacccggcg agcgccccca ccatcatggc caacatgagc aacatcggct
    10261 ggtttggcga caccgtggcg atcgaccagc acctggccat cagccgcctg cgcgcgctgg
    10321 agttcgagcg tcccatgctg cgcgccacca acaccggcgc cacggccgtg atagaccatc
    10381 gcgcgcgcgt cacccaccgc ctgccagcct tccagcgcaa ggcgctcaac gcccaggtgc
    10441 aggggcgcgg cggcgccgtc acgccctatg cctggtgggt gtcgcgctgg ggactggcgc
    10501 cactgtgggc gctgggcctg ggcctggccg cctgggcctg gctgcgccgg cgcaggcatt
    10561 gaccgcggcg ggcacaaagg gcaaggtata tctagccagg ctatcgggac actggataat
    10621 ggacactttt caaggccctg cggtgtgggg cctaccctct acaaaaatca caagcatcaa
    10681 tggctgattc cttttcctac gaacaactga ttgcttccgg cgagggccgc ttgttcaacc
    10741 ccgacagtgg ccgcctgccc ctgcccccga tgctgatgtt cgaccgcatc acgcatatcg
    10801 acgccgacgg cggcgcgcac ggcctgggca agatcgtggc cgaactggac gtcaagcccg
    10861 acctgtggtt cttcgactgc cacttccagg gcgacccggt catgcccggc tgcctggggc
    10921 tggatgccat gtggcagct
//
LOCUS       DAOMTQ010000103        10757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1182_length_10757_cov_28.418240, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000103 DAOMTQ010000000
VERSION     DAOMTQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10757
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_10757_cov_28.418240"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /gene="gyrB"
                     /locus_tag="PK518_10545"
     CDS             <1..944
                     /gene="gyrB"
                     /locus_tag="PK518_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592519.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRM49397.1"
                     /translation="IIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKV
                     KNGKEELYLKDAAALDGFLLRVALNNASVSTGGEAARVLSGEELAALARTHQHAEHVI
                     TRLSTFMDAEALRAAADGVAFRLDTLEDAQASAIELQAKLRELNVNGVPADVTGEIDP
                     RSDKPVLRISRHHHGNIKSSLITQDFVAGDDYATLAEAANSFRGLIQEGAKVTRGEGD
                     KAKEEKVADFREAMAWLISEAERTTARQRYKGLGEMNPEQLWETTMDPAVRRLLRVQI
                     DDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV"
     gene            944..2500
                     /locus_tag="PK518_10550"
     CDS             944..2500
                     /locus_tag="PK518_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HRM49398.1"
                     /translation="MAAEPSHHIPPPEATRAQPAALSLPQQRTQQYILARQQHPAWLL
                     LASRRAPLMLSCLESLLEHQPGGVPFDSAVQAIADLLVQHANQPDYEIDAADPLAQAR
                     RELREWIRRTLVTERDGLIHETDALKSALRFVAQLDQRMMTSTASRLAVVQREIDNLA
                     TQLDPDPASRAAQLQRRIAELQQQIDALQAGHVETLSDAQASEGIREVYALATSLRAD
                     FRRVEDSWREADRQLRQAILSAQQHRGSIVDQLLDGHANLLSTPEGRVFEGFQQQLHH
                     QAELARMRQHLRTILAHPASRQALDDLQHSALRLLVPQLIKEAKVVQAVRARSEREVA
                     QFMKTGQAAENQRVGQLLNDILNQALQLDWSRHALRRSPAPLPPLGVALAGLPLVERL
                     RVKSLEEGAAQELLLTTQYADLSQVEDEFWQAFEGLDTKALQQDTLALLEARGAPLTM
                     ADLAQALPPGEHDLETLAQWLAWACEAGAEVDAQAKQQIELQRANGQLCRFTVPRAEL
                     TASALRGVES"
     gene            2691..2882
                     /locus_tag="PK518_10555"
     CDS             2691..2882
                     /locus_tag="PK518_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49399.1"
                     /translation="MAGAIARRATIVTWLCGLGALCTFVPYLPGTLMPDSLRWLAGLA
                     SHWQWLYAGLALTGAVLTA"
     gene            2925..3650
                     /locus_tag="PK518_10560"
     CDS             2925..3650
                     /locus_tag="PK518_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRM49400.1"
                     /translation="MVAAAWLHQLPQAEARTAHNQSSVTLTMASANLNFERTDHAALA
                     AWLLSAEAPDAIALQEFTPSAMAMVNRPEVLAAYPHRVLEPSDDQFGLGVLSKYPIAS
                     AEKVPAADVLATLKLRLVLDVQGQKLALTAVHPMPPISAPYAQERDASLRLEARLLAD
                     SGLPGILLGDMNDSPWSTGLQATAPLRRASGLAPTWPNLGGWLSVLPLDHVLVTPGVR
                     ADGAGWGVDLGSDHRPVRVRLAL"
     gene            complement(3685..4449)
                     /locus_tag="PK518_10565"
     CDS             complement(3685..4449)
                     /locus_tag="PK518_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRM49401.1"
                     /translation="MSLLMPEHEHRVAVLVDCDNTSPEILEYALRVVAQFGRVVLRRG
                     YGNHSTLANKWQEALVRLAFTPCLQYQYAAGKNTSDIALALDAMEALFDRRATTFCLV
                     TSDSDFAYLCRKLRERGATVHIVGEAKSPDALRNASDQFFEWVKPVPEPVASVAAAEL
                     ADKGSSKPEPAKPAPKLRPRFVVEAVALMAGDTADGKVSVGGLGSYLKRADPSFSPKN
                     YGHSGLLDMLKTYDLLKVQQEAGGHWTTQLAQPSQS"
     gene            complement(4523..4783)
                     /locus_tag="PK518_10570"
     CDS             complement(4523..4783)
                     /locus_tag="PK518_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM49402.1"
                     /translation="MTTLSDVALLLRTARKQARLTQAELAERAGVARTTVARMENPSK
                     GDMSVSALVRLLEAAGCDLKVVPVGHQRTVEEILAEQRRGDT"
     gene            4993..5268
                     /locus_tag="PK518_10575"
     CDS             4993..5268
                     /locus_tag="PK518_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HRM49403.1"
                     /translation="MKTSTIPSLRVDPQLRLAAESVLRDGESLSGFVEQAIRQTIACR
                     LADQEFITRGLKARDQARQSGQYVEAGAVLDRLQEMLDRAKSGPTTA"
     gene            5265..5573
                     /locus_tag="PK518_10580"
     CDS             5265..5573
                     /locus_tag="PK518_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM49404.1"
                     /translation="MTYRVRFTQEAEADLVRLYEFILDRDAADWALAERALETIRAGI
                     ATLERLPFTCRKATADSPFLRELVIPFGAAGYVALFEIDDAQTVTVLALRHQRESDYH
                     "
     gene            5711..6025
                     /locus_tag="PK518_10585"
     CDS             5711..6025
                     /locus_tag="PK518_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM49405.1"
                     /translation="MAISAADVIPLSQARATLSELADQVRAGAEKIITKNGESYVALI
                     DAGRLDYYHQLERERIHLLLIADASQGLADVAAGKVKDARATLAAMQRRRATHRGGKA
                     VD"
     gene            6042..6419
                     /locus_tag="PK518_10590"
     CDS             6042..6419
                     /locus_tag="PK518_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM49406.1"
                     /translation="MTVKLTANFERNLADIEAFLIEAEAPQAFDALLDELARTTIPNL
                     ERFPGMGRLFLERPVRSVEVANGIARLTQQLGALATDGELREYVMAHYLLLYARIGGT
                     VYLLSIRHQRQLSFDFQALWQSH"
     gene            6506..7222
                     /locus_tag="PK518_10595"
     CDS             6506..7222
                     /locus_tag="PK518_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HRM49407.1"
                     /translation="MPTAPDLFDQWAAAPQASPAVDAQPAPEPPPEPAPEAVPATPQP
                     LRRALQELLKMGLLEQASKPQLFRHIAIDTARVNALLEPLDLQVRVDDLRGLAYVTVA
                     PGWQAGEAEDDDEWTHPLVRRQRLTLEQSLLLAILRREFLQREQEGGIGRPVQIAVDS
                     LLPQLEIYLGSTGSDMQERKRLAALLEHLRGHGVVSDVDAQERITIRPMIAHLANPEN
                     LQALLTQLRALSADSTGNAT"
     gene            7219..10500
                     /locus_tag="PK518_10600"
     CDS             7219..10500
                     /locus_tag="PK518_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM49408.1"
                     /translation="MSSASYRLHALHLYNWGAFAGRHQASISPDNSAIIGPTGSGKTT
                     LIDALMTLLCQHPRYNLASTGGHESDRDLVSYLRGASGPGHEAEGSAHLARQGRCVTG
                     LAAQLRGADDDGAGDIWLGAILWLDGSSSAQADMGRRWFFAQGGGHSLDLWLEEHHSG
                     GARALSRLVKNSDGLEMFSSKSAYLARVQRFFEIGPNAFTLLNRAAGLKQLNSIDEIF
                     RDLVLDDHSAFDDALRVADSFDELAAIHAELELANRQCLALLPLRELAQQEARQQQAL
                     QDQRLLHAALPHWCARLGQQQWAARCADLQQQLNAQAQRISAQEQQLEQARQQEQTLL
                     EAYLRAGGGDIELLRRSIATQQDQCRQRAQHWAQFQQLAGQIDLPVAQPFGAAQLAQL
                     QAQAAPALAQLQNQAAALQEALEDAVAQERNAHAALAQLQDEITAVRQRPGSNLPPDF
                     TQFRAQLAEQLRLAPDALPFVAELVEVPAAHQAWRGAIERALGAQRLRLLVPPQAMQA
                     ALAWVNQRHNRLHVRLLEVRAPASPARFWDDGFARLLTLKSHAHQGALRQLLAEQDRH
                     CVASTEALQTTPHAMTREGLMSGRAQHFDKQDQRRIDQDWMTGFDNRDRLAQLQERLQ
                     AAQGQWETLQKQKLLAQSQQALEAQKAQSWQTLQGLHWSDIDQAGAQAELQQLQTRVA
                     ALLDPQSGTVQAQAAWQAAQQHGQAQAQALLRLQTAQATWQERQASAQTQERLCRERL
                     AATPAPPGLDTLHGPLAALCPPGTACEELATQERQALASASERLDRLGHRLALLHKDI
                     IRQMAAAQREDRGALTDHPQEVEALAHYLQRLQRLQEEDLPAKRARFQDYLNQASDQG
                     VSTLLSGIEAQVAEITERIDQLNQTLARVDFHSGRYLQLVPQPVLHPSLQQLHQARAQ
                     LRSERLQDDGGQRHYQALCAMVELLREHASNRRTKAAQALLDARYRLQFAVHVLDRAT
                     GQVLERRTGSQGGSGGEKEIIASYVLTASLSYALCPPGRSQPLFCTIVLDEAFSKSSQ
                     AVAARIIQALQEFGLHALFVTPNKELRLLRQHTRSAVLVHRRGAQALLANMGWEEIAA
                     RAAGR"
BASE COUNT         1902 a         3834 c         3453 g         1568 t
ORIGIN      
        1 tcatcatcat gaccgacgcc gacgtggacg gcgcccacat ccgcaccctg ctgctgacct
       61 tcttctaccg ccagatgccc gagctggtcg agcgcggcca tatctacatc gcccagccgc
      121 cgctgtacaa ggtgaagaac ggcaaggaag agctgtacct gaaggacgcc gcggcgctgg
      181 acggctttct gctgcgggtg gcgctgaaca acgccagcgt cagcaccggc ggcgaggcgg
      241 ctcgcgtgct gagcggcgag gagctggccg ccctggcacg cacgcaccag catgccgagc
      301 atgtcatcac ccgcctgtcc accttcatgg atgccgaggc cctgcgcgcc gccgccgacg
      361 gcgtggcctt ccgcctcgac accctggaag atgcccaggc cagcgccata gagctgcagg
      421 ccaagctgcg cgagctcaac gtcaacggcg tgcccgccga cgtgaccggc gagatcgacc
      481 cgcgcagcga caagcccgtg ctgcgcatca gccgccacca ccacggcaac atcaagagca
      541 gcctgatcac gcaggacttt gtcgccggcg acgactacgc caccctggcc gaggccgcca
      601 acagcttccg cggcctgatc caggagggcg ccaaggtcac gcgcggcgag ggcgacaagg
      661 ccaaggaaga aaaggtggcc gacttccgcg aagccatggc ctggctgatc agcgaggccg
      721 agcgcaccac cgcccgccag cgctacaagg gcctgggcga gatgaacccc gagcagctct
      781 gggaaaccac catggacccg gccgtgcggc gcctgctgcg cgtgcagatc gacgacgcca
      841 tcgaggccga ccgcgtgttc accatgctca tgggcgatga ggtggagccg cggcgcgact
      901 tcatcgagac gaatgcgctc agggcgggga acatcgatgt gtgatggcgg ccgagcccag
      961 ccaccacatc cccccccctg aggccacacg ggcgcagcct gccgcgctct ccctgcccca
     1021 gcaacgcacc caacaataca tcctggcgcg ccagcagcac ccggcctggc tgctgctggc
     1081 ctcgcgccgg gcgccactca tgctcagctg cctggaatcg ctgctggagc accagcccgg
     1141 cggcgtgccc ttcgacagcg ccgtgcaggc cattgccgac ctgctggtgc agcacgccaa
     1201 ccagcccgac tatgagatcg acgccgccga cccactggcc caggcgcggc gcgagctgcg
     1261 cgagtggata cgccgcaccc tggtcaccga gcgcgacggc ctgatccacg agaccgatgc
     1321 gctcaagtcg gcgctgcgct tcgtcgccca gctggaccag cgcatgatga cctccaccgc
     1381 ctcgcgcctg gccgtggtgc agcgcgagat cgacaacctg gccacccagc tcgaccccga
     1441 cccggccagc cgcgccgcgc agctgcagcg ccgcattgcc gagctgcagc agcagatcga
     1501 cgcgctgcag gccggccatg tcgagaccct gagcgacgcc caggccagcg aaggcatacg
     1561 cgaggtctat gccctggcca ccagcctgcg cgccgacttc aggcgtgtgg aagactcctg
     1621 gcgcgaggcc gaccgccagc tgcgccaggc catcctgagc gcgcagcagc accgcggcag
     1681 catcgtcgat caactgctcg acggccacgc caacctgctc tccacccccg aggggcgggt
     1741 cttcgagggc tttcagcagc agctgcacca ccaggccgag ctggcgcgca tgcgccagca
     1801 cctgcgcacc atattggccc acccggccag ccgccaggcg ctggacgacc tgcagcacag
     1861 cgccctgcgc ctgctggtgc cccagctcat caaagaggcc aaggtggtgc aggccgtgcg
     1921 cgcgcgcagc gaacgcgagg tggcgcagtt catgaaaacc ggccaggcgg ccgaaaacca
     1981 gcgcgtcggt cagctgctca acgacatcct caaccaggcg ctgcagctgg actggagccg
     2041 ccatgccctg cgccgcagcc cggcgccgct accgcccctg ggcgtggcgc tggcgggcct
     2101 gccgctcgtc gagcgcctgc gcgtcaagtc gctggaggag ggcgccgcgc aagaactgct
     2161 gctcaccacg caatacgccg acctgagcca ggtggaggac gagttctggc aggccttcga
     2221 gggcctggac accaaggcgc tgcagcagga cacgctggca ctgctggagg cccgcggcgc
     2281 ccccctgacc atggccgacc tggcccaggc cctgccgccg ggcgagcacg acctggaaac
     2341 cctggcccag tggctggcct gggcctgcga ggcgggcgcg gaggtggatg cacaggcaaa
     2401 gcagcagatc gaactgcagc gcgccaacgg ccaactgtgc cgcttcacgg tgccgagggc
     2461 ggagctgacg gcgagtgcgt tgcggggggt ggagagctga aattggcggt atgcttctca
     2521 agttacaaaa acatacgact gcttggaggc cgctcttggc gcccccggtc gatgcaccac
     2581 agccaccctg agaagacccg cacttgaaac cctggattgc caccgccaat accgtaagtc
     2641 gtaccgggtt ggcaccatcg ccccggcagc gcagccccct gaaagcccgc atggccggcg
     2701 ccatcgcccg gcgcgccacc atcgtgacct ggctgtgcgg cttgggcgcc ctgtgcacct
     2761 tcgtgcccta cctgccgggc accctcatgc cggacagctt gcgctggctg gccgggctgg
     2821 catcgcattg gcaatggctc tacgccggcc tcgccttgac cggggccgtg ctcaccgcct
     2881 gactgcggcc ggcacgcccc tggcaggctc tggcgcctgt ggccatggtg gcggccgcct
     2941 ggctgcacca gctaccccag gccgaggccc gcacagcgca caaccagtcg tcagtcacgc
     3001 tcaccatggc cagcgccaac ctgaacttcg agcgcaccga ccatgccgcg ctcgccgcct
     3061 ggctgctttc ggccgaggcg ccggacgcga tcgcgctgca ggagttcacc ccctccgcca
     3121 tggccatggt gaaccgcccc gaagtgctgg ccgcctaccc ccaccgcgtg ctcgaaccca
     3181 gcgatgacca gtttgggctg ggcgtgctgt ccaagtaccc gatcgcatcg gcggaaaagg
     3241 tgcccgccgc cgacgtactg gcaacgctca agctgcgcct ggtgctggac gtgcaaggcc
     3301 aaaaactggc cttgaccgcc gtgcatccca tgccgcccat cagcgcaccc tacgcccagg
     3361 agcgcgatgc cagcctgcgg ctggaagcca ggctgctggc cgacagcggt ctgcccggca
     3421 tcctgctggg cgatatgaac gactcgccat ggtccaccgg cctgcaggcc accgccccgc
     3481 tgcgccgcgc cagcggcctg gcgcccacct ggccgaacct gggcggctgg ctgtccgtcc
     3541 tgccgctcga tcatgtgctc gtgacgccgg gagtgcgggc ggatggcgcg ggatgggggg
     3601 tggatttggg gagtgatcat cggccggtgc gggtgcgttt ggcgctgtag cgcaatcaac
     3661 ggccccttgc tgtgaactga ccgttcaact ttgtgatggc tgcgccaact gcgtcgtcca
     3721 atgcccaccg gcttcctgct gcaccttcag caagtcgtag gttttgagca tgtccagcaa
     3781 acctgaatga ccgtagttct tgggagaaaa agaagggtcg gcacgcttga gataagagcc
     3841 caggccgcct acgctgacct ttccatcggc cgtgtctcct gccatcagcg caaccgcctc
     3901 taccacgaag cgcgggcgca gtttgggtgc gggcttggcc ggctcgggtt tgctgctgcc
     3961 tttgtctgcg agctctgccg cagccaccga ggccaccggc tccgggactg gcttgaccca
     4021 ctcgaagaac tggtcgctgg cattgcgcag cgcatcgggc gacttggctt cgcccacgat
     4081 atgcaccgtg gccccacgct cgcgcagctt gcggcacaga taggcaaagt cggaatcgct
     4141 ggtgacgagg caaaacgttg tcgcccggcg gtcaaacaat gcttccatgg catccagtgc
     4201 gagggcgatg tccgaggtat ttttgcccgc cgcgtattgg tattgcaagc aaggcgtgaa
     4261 cgccagccgc accagggcct cttgccactt gttggccaag gtgctgtggt tgccatagcc
     4321 gcgccgcagc accacacggc caaactgcgc caccacgcgc aaggcatatt ccaggatttc
     4381 cgggctggtg ttgtcgcagt ccaccagcac cgccacgcgg tgttcatgtt caggcatcaa
     4441 gaggctcatg cttgaggact ccttatcgag agtcgcattc attcattgct ataggtgtac
     4501 gccgtgcctc tgccctgcag cgtcacgtat ccccccggcg ctgctcggcc agaatctctt
     4561 ccaccgtgcg ctgatggccc acgggcacca ctttcaggtc gcagccggcg gcctccaaca
     4621 gtcgcaccag ggcggaaaca ctcatgtcgc ccttggaggg gttttccatg cgcgccacgg
     4681 tggtgcgcgc cacgccggcg cgctcggcca gttcagcctg ggtcaggcgc gcttgcttgc
     4741 gggcagtgcg cagcagtagg gcaacgtcag agagggtggt cattgtggct tgtgggcgtc
     4801 attattttca gctggcacct caaatgtggc ttatgagcat cttttgtaaa cccctgggtt
     4861 gcgggatgag ggggcgcgaa ccaaagggcg gtaccgcaag tttgtcgtca ctctcgccct
     4921 ttcccttacc gcttgcgcag cggttgaaac atgggcacac tgcatcgcaa ttgcaacgca
     4981 cggagccttt tcatgaaaac ctccaccatc ccatctttgc gggttgaccc gcagctgcgt
     5041 ctggccgcgg aaagtgtttt gcgcgatgga gaaagtctgt cgggcttcgt agaacaggcg
     5101 attcggcaaa ccatcgcctg ccgtctggcg gatcaagagt tcatcacccg tggcttgaag
     5161 gcgcgcgacc aggcgcggca gagcgggcag tatgtggagg ctggcgccgt gctggacagg
     5221 cttcaggaga tgcttgatcg cgccaagtcc ggcccaacca ccgcatgacc taccgggttc
     5281 gctttacaca ggaagccgag gccgatctgg tcaggctgta tgaattcatc cttgacaggg
     5341 atgccgcaga ctgggccttg gccgagcgtg cgctggagac aattcgcgcg ggcattgcca
     5401 cgctggagcg cctgcccttt acctgccgca aggccacggc tgactcacct tttttgcggg
     5461 agttggtcat cccgtttgga gcggccggct atgtcgcact gtttgaaatc gacgacgccc
     5521 aaaccgtgac ggtgctggcg ctgcgccatc agcgagagag cgactaccac tgatcgcgct
     5581 gcgccttcgc cacacactgt gctggcaagg cacaccccga gccgtgaact cgcagtcatt
     5641 tccaacagcg acagccttgc gcatatgatg tacaaaattc cgtacatcaa aacacgccaa
     5701 ggaggctccc atggccatct ccgccgctga cgtgatcccg ctctcccaag ccagagccac
     5761 cctgtcggaa ctggccgacc aggtgcgggc tggcgccgag aagatcatta ccaagaatgg
     5821 cgaaagctac gttgccctga tcgacgcggg gcggctggac tactaccacc agctcgaacg
     5881 cgagcgcatt cacctgctgc tgatcgccga tgccagccag ggtttggccg atgtggctgc
     5941 gggcaaggtg aaggacgcac gcgccacctt ggcggccatg cagcgccgcc gcgcgaccca
     6001 tcggggtggc aaggctgttg actgactgac tgccttgcac cgtgaccgtc aagctcactg
     6061 ccaactttga acgcaacctc gccgatatcg aagccttctt gatcgaggca gaggctcccc
     6121 aggccttcga tgctctgctc gatgagttgg cgcgtaccac catcccgaat ctggagcgct
     6181 ttccaggcat ggggcgcttg tttctcgaac ggccggtgcg ctcggtcgag gtggccaatg
     6241 gcatcgcacg cttgacccag caactgggcg cactcgccac cgacggcgag ctgcgcgagt
     6301 acgtgatggc gcattacctg ctgctgtatg cgcgcatcgg gggcacggtg tacctgctct
     6361 ccatccgcca ccagcggcag ctctcgtttg attttcaggc gctgtggcaa agtcattgac
     6421 gcggcacaac atgttgctac ccaagggcga aaaccagttg taccgtgcaa ccactctgcg
     6481 caaacagctc tttattcaat agcaaatgcc caccgccccc gacctcttcg accaatgggc
     6541 cgccgcgccg caggccagcc ccgccgtgga tgcgcagcca gcccccgagc cgccgcccga
     6601 acccgcgcca gaagccgtcc ccgccacccc ccagcccctg cgccgcgccc tgcaagagct
     6661 cttgaaaatg ggcctgctgg agcaagccag caagccgcag ctgtttcgcc acatcgccat
     6721 cgacacggcg cgcgtcaacg cgctcttgga gccgctggac ctgcaggtgc gcgtggacga
     6781 cctgcgcggc ctggcctatg tgacggtggc gcccggctgg caggccggcg aggccgagga
     6841 cgatgatgaa tggacccacc ccctggtgcg ccgccagcgc ctgacgctgg agcagtcgct
     6901 gctgctggcg attctgcggc gcgagttttt gcagcgcgag caggagggcg gcatcggccg
     6961 cccggtgcag atcgccgtgg acagcctgct gccgcagctg gagatttacc tgggcagcac
     7021 cggcagcgac atgcaggagc gcaagcgcct ggccgcgctg ctggagcatt tgcgcggcca
     7081 cggcgtggtg tcggacgtgg acgcgcagga gcgcatcacc atccgcccca tgatcgccca
     7141 cctggccaac cccgagaacc tgcaggccct gctgacgcag ctgcgcgccc tgagtgcgga
     7201 cagtacaggc aacgcaacat gagcagcgcc agctaccgcc tgcacgccct gcacctgtac
     7261 aactggggcg cctttgccgg gcgccaccag gccagcatct cgccggacaa cagcgccatc
     7321 atcggcccca cgggcagcgg caagaccacg ctgatcgacg cgctgatgac gctgctctgc
     7381 cagcacccgc gctacaacct ggcctccacc ggcgggcacg agagcgaccg cgacctggtg
     7441 tcctacctgc gcggcgccag cggcccgggc cacgaggccg agggcagcgc ccacctggcg
     7501 cgccagggcc gctgcgtgac cggcctggcg gcgcagctgc gcggtgcgga cgacgacggc
     7561 gcgggcgaca tctggctcgg cgccatcctg tggctggacg gcagcagcag cgcccaggcc
     7621 gacatggggc ggcgctggtt ctttgcccag ggcggcggcc acagcctgga cctgtggctg
     7681 gaggagcacc acagcggcgg cgcgcgcgcc ctgagccgcc tggtgaagaa cagcgacggc
     7741 ctggagatgt tcagcagcaa aagcgcctac ctggcgcggg tgcagcgctt tttcgagatc
     7801 gggcccaacg ccttcaccct gctcaaccgc gcggccgggc tgaagcagct caacagcatc
     7861 gacgagatct ttcgcgactt ggtgctcgac gaccacagcg cctttgacga cgccctgcgg
     7921 gtggccgaca gttttgacga gctggccgcc atccacgccg agctggagct ggccaaccgc
     7981 caatgcctgg ccctgctgcc gctgcgcgag ctggcgcagc aagaggcgcg ccagcagcag
     8041 gctctgcaag accagcgcct gctgcacgcc gccctgccgc actggtgcgc gcgcctgggc
     8101 cagcagcaat gggcggcgcg ctgcgccgat ctgcagcaac agctgaacgc gcaggcgcag
     8161 cgcatcagcg cccaggagca gcaactggag caggcgcgcc agcaggagca aaccctgctc
     8221 gaagcctatc tgcgcgccgg cggcggcgat atagagctgc tgcgccgcag catcgccacc
     8281 cagcaagacc agtgccgcca gcgcgcgcag cactgggcgc agttccagca gctggccggg
     8341 cagatcgacc tacccgtcgc ccagcccttt ggcgctgcgc aactggcaca gctgcaggcc
     8401 caggccgcgc ccgcgctggc gcaattgcaa aaccaggcgg ccgcgctgca agaggcgctg
     8461 gaagacgccg tggcgcagga gcgcaacgcg cacgccgcgc tggcgcaact gcaggacgaa
     8521 atcaccgccg tgcgccagcg gccgggctcc aatctgccac cggacttcac ccagttccgc
     8581 gcccagctgg ccgagcagct gcgcctggcg cccgacgccc tgcccttcgt cgccgagctg
     8641 gtcgaagtgc ccgccgccca ccaggcctgg cgcggcgcca tagaacgcgc cctgggcgcg
     8701 cagcgcctgc gcctactggt gccgccgcaa gccatgcagg ccgccctggc ctgggtgaac
     8761 cagcgccaca accgcctgca tgtgcgcctg ctggaggtgc gcgccccggc cagcccggcc
     8821 cgcttctggg acgacggctt tgcccggctg ctgaccctga agtcccacgc ccaccagggc
     8881 gcgctgcgcc agctgctggc cgagcaggat cgccactgcg tggccagcac cgaggccctg
     8941 cagaccacgc cccacgccat gacgcgagag ggcctgatgt ccggccgcgc ccagcatttc
     9001 gacaagcagg accagcgccg catagaccag gactggatga ccggctttga caaccgcgac
     9061 cgcctggcgc agctgcaaga gcgcctgcag gccgcgcaag gccaatggga aacgctacaa
     9121 aaacagaagc tgttagcgca atctcagcaa gcgctagagg cccaaaaggc ccaaagctgg
     9181 caaaccctgc agggcctgca ctggagcgac atcgaccagg ccggcgccca ggccgagctg
     9241 caacagttgc aaacccgggt ggccgcgctg ctcgatccgc agtccggcac cgtccaggcg
     9301 caggccgcct ggcaggccgc ccagcagcac ggccaagccc aggcccaggc gctgctgcgg
     9361 ctgcaaaccg cccaggccac ctggcaggaa cgccaggcca gcgcccagac gcaagagcgc
     9421 ctgtgccgcg agcgcctggc cgccaccccg gctccgcccg gtctggacac gctgcatggc
     9481 cccctggccg ccctgtgccc gccgggcacc gcctgcgaag aactcgccac gcaagagcgc
     9541 caggccctgg ccagcgccag cgagcgcctg gacaggctgg gccacaggct ggccctgctg
     9601 cacaaggaca tcatccggca gatggcggcg gcgcaaaggg aagaccgcgg cgcactcacc
     9661 gatcacccgc aagaggtcga agccttggcg cattacctgc agcgcctgca gcggctgcag
     9721 gaggaagacc tgcccgccaa gcgcgcgcgc ttccaggact atttgaacca ggcctccgac
     9781 cagggcgtga gcacgctgct gtccggcata gaggcgcagg tggccgagat caccgagcgc
     9841 atagaccagc tcaaccagac cctggcgcgg gtggactttc acagcggccg ctacctgcag
     9901 ctggtgcccc agcccgtgct gcaccccagc ctgcagcagc tgcaccaggc gcgcgcccag
     9961 ctgcgcagcg aacgcctgca ggacgacggc ggccagcgcc actaccaggc cctgtgcgcc
    10021 atggtcgagc tgctgcgcga gcacgccagc aaccgccgca ccaaggccgc ccaggccctg
    10081 ctggacgcac gctaccgcct gcagttcgcc gtccacgtac tcgaccgcgc cacgggccag
    10141 gtgctggagc gccgcaccgg ctcacagggc ggcagcggcg gcgagaagga aatcatcgcc
    10201 agctacgtgc tcaccgcgtc gctgagctac gcgctgtgcc cgcccgggcg cagccagccg
    10261 ctgttctgca ccatcgtgct ggacgaggcc ttctccaaaa gctcccaggc cgtggccgcg
    10321 cgcatcatcc aggcgctgca ggaattcggc ctgcacgcgc tcttcgtcac ccccaacaaa
    10381 gagctgcgcc tgctgcgcca gcacacccgc agcgccgtgc tggtgcaccg ccgcggcgcg
    10441 caagcactgc tggcgaacat ggggtgggag gagattgcgg cgcgggcagc ggggcggtga
    10501 gttggcgggc gatgccacat cggtatccat tgggcatggt caggaaattg gcgtttgaat
    10561 ccgtccctga acttcaggcg caccattcaa gtgacggttg aagaccacgg caccgaagct
    10621 gttcatgcag gtactacgct agtacagcga cacggaggta tcggaacaag atgaaagcga
    10681 tggattcgcg cccagggcaa ggcgcaaacc gcatcgatag ccgtagctat cgcgaggatt
    10741 tgcaacgccg ccatggg
//
LOCUS       DAOMTQ010000104        10686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1197_length_10686_cov_28.049760, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000104 DAOMTQ010000000
VERSION     DAOMTQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1197_length_10686_cov_28.049760"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK518_10605"
     CDS             <1..201
                     /locus_tag="PK518_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRM49409.1"
                     /translation="IYKRVRHSQTRPLLQVADPQARLRELYRTRDPLYREAAHYVIET
                     GRPTVNTLVNMIVMQLEMAPGA"
     gene            226..4290
                     /locus_tag="PK518_10610"
     CDS             226..4290
                     /locus_tag="PK518_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRM49410.1"
                     /translation="MTSAPPILHGPDRPELLRGEVLADLFEATAARLPHKTALIAGGE
                     HLSYGALNAAADLAAHRLILAGLRPGDMVGLWLPRGLELLICQLAIAKTGAAWLPFDA
                     EVPTERIAICLDDANASALLISELSAPAIRAQTGISAQILTAQAVLAPLPAGEPLRRR
                     ADAAPEHTAYVIYTSGSTGKPKGIAVTQASICHFLRSENARLGVREDDRVYQGFSVAF
                     DMSFEEIWISYLVGATLWLAPKEIAGDPEALPRALIENDITVLHAVPTLLALFAQDVP
                     GLRIINLGGEMCPQALVDQWATPGRQMFNTYGPTEATVSASLAELHAGEPVTIGEALP
                     NYGLLVIEVIEPEAIVDGRLSPLKLLPFGETGELCITGPGVAAGYLGRPELTAEKFLP
                     NPWARNDCEARLYRTGDLARVELRADGQPQMQCLGRADDQVKVRGFRVELGEIEAVLA
                     AQPGVGTTAVVLRPDAGMDQLIAFYVPNGQPPEAKTLRAALADRLPPYMVPARFEDLA
                     AMPRLSSGKIDRKVLKSQPLAAAAPAEGGDEPETPAETALFAALQGLFPGQPLRRAQD
                     FFSDLGGHSLLAARLVSRLRQDACFARATVADIYTHRRLGAIAQALQAGMVDARAGAA
                     ERPFLIHGRWRRWRCGLAQGLAIPLLVLLNMAQWLAPFFAYHFYTGDAGDSITRAVAM
                     SVLAFLASTLLGFGVATTGRWLLAGRLAPGSYPLWGLTYYRWWLADRLTETVPVYLLN
                     GSALYPAWLRLLGARIGPEVVLGSTTMRVPHLVSIGAGTSIGNGVNLENARVEGGRLH
                     LGAIDIGANACIGSYVVIEGDTVVGDWAHVEGQSALAAGQRVPARQAWHGSPARQRGD
                     FDPASLPARPAVSAARRAGEVTFFALAALAVAVLFFMPVFPTFMLIDWLDIPEISVRP
                     LVDDGSISALQAFGLRLVKFFVLALPASLVFILVTVLLSAGIRWALLPRMRAGSWPVH
                     SPRYLAKWTVNLIQEASLAVLHGIYATVYSAAWYRLLGARVGKETELSTALGVVPDML
                     TLGDGCFIADAVMLGDEHIDGGWMTVQPTVVSRRSFIGNGAYVPDGTTVPENVLIGVL
                     SSVPRNASMQDGDTWLGAPPLRLPAREQVAGFPEHLTFAPSPWRKAARGLVEAFRIAA
                     PHALVIATGYAIVLDAMPLAEEGRWAAVALDLAVGGMLFGMATFGFVALFKWLAVRRY
                     GQRAAPMWTPFVWLSEAATNLYEGVAVPWFLRYLRGTPWLNDALKLLGADIGRGVFLD
                     TTDMTEFDCVHIGAHSELNALCCPQTHLFEDRVMKIDHVRIGSRVTLGPRCTVLYGAQ
                     VGDGAQLGPLTLVMKGEQIPAGTLWHGLPAAPWR"
     gene            4426..5043
                     /locus_tag="PK518_10615"
     CDS             4426..5043
                     /locus_tag="PK518_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRM49411.1"
                     /translation="MPCPFAGDRLAGAPQAHALDTRLAQAVAPGQERDHARAQARAAL
                     RRLLAAVLGCAPQALSVSDARGIPPRLGWTTGGRSGLDAIGLSISHAPGLSLVAWCPK
                     GAVGVDVQAVPYATDRAELWRTAALFFPPDRLEALAEHASDALFSEAFAGHWAAHEAA
                     LKCLGQGLVEYTPTLAARLAGVRTAALTLPGWAAADVVAAIAWRP"
     gene            complement(5200..5721)
                     /locus_tag="PK518_10620"
     CDS             complement(5200..5721)
                     /locus_tag="PK518_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HRM49412.1"
                     /translation="MRFLSHAALGASVLLLTACGHLAGQGLGAGTSLQPRSGSATSGQ
                     ANFRQQGDRVLVRVEVSGLTPGQEHGLHIHDKGDCSAPDAMSAGGHFNPTGKPHGPQN
                     ADHHGGDLPALKADASGKARAEFSVTGVTVAPGPTSIVGRSLVVHKDADDYKTQPTGN
                     SGARIACGVIAAS"
     gene            5866..7197
                     /locus_tag="PK518_10625"
     CDS             5866..7197
                     /locus_tag="PK518_10625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM49413.1"
                     /translation="MPDSTGNCDFIALSNDSYRLLLDEVGAFVYTTDMVGRYTFANRM
                     VLQLLGHPLDYVVGKDISHFFGDKGNEALRETDERVLRGGETIAREESNLILATGELR
                     TYWSTKKPLRDATGQIIGMIGISHDITEKKRLEDKLRRQKELLDALVDNVDALIYMKD
                     ANRRFLYANRRMAEAFGCSVDQIIGSLDVDLMPAEVAERFWQQDQKILANGQRHACEE
                     SLVDASGRLRHYWSVIIPWAGPDGTPAIIGLSTDITELHALKEELQRQARADSLTGIA
                     NRRSFYENAELEFARSRLQGQALSLITIDIDFFKKINDTLGHPMGDRVLQEFVACCQD
                     ALRNDGLLARTGGEEFCILLPGTDLDTARHIAERIREQTQARRIAVPAQELRISASFG
                     VAGLCDTDERFETLYSRADLALYAAKQQGRNRTSLLRSPAQEGVLATPVAD"
     gene            complement(7194..9938)
                     /gene="metH"
                     /locus_tag="PK518_10630"
     CDS             complement(7194..9938)
                     /gene="metH"
                     /locus_tag="PK518_10630"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRM49414.1"
                     /translation="MTAAAPTPVPPMKLSGLEPVFIGADSLFVNVGERTNVTGSKAFA
                     RMILNEQYEEALSVARQQVENGAQVIDVNMDEAMLDSKAAMVRFLNLIASEPDIARVP
                     VMVDSSKWEVIEAGLRCLQGKGIVNSISMKEGVEPFKQQAKLIKRYGAAAVVMAFDEQ
                     GQADTFARKTEICERAYRVLVDEVGFAPEDIIFDPNIFAVATGIEEHANYAVDFIEAV
                     RWIKQNLPGAKVSGGVSNVSFSFRGNDPVREAIHTVFLYHAIAAGMDMGIVNAGMVGV
                     YDDLEPELRERVEDVVLNRRPDAAERLLEIAESAKGAAKDEGKKLEWRGTPEHPKTVG
                     ERLSHALVHGITDFIVEDTEEAYQDIVVAKGGRPLHVIEGPLMDGMNIVGDLFGAGKM
                     FLPQVVKSARVMKQAVAHLIPYIEEEKRQQEAAGLDVSSKGKIVIATVKGDVHDIGKN
                     IVTVVLQCNNFEVINMGVMVPCHEILARAKAEGADIVGLSGLITPSLEEMQYVAAEMH
                     KDDHFRVKKIPLLIGGATCSRVHTAVKIAPNYDGPVVYVPDASRSVSVAQSLLGEGKD
                     AYLAEVAADYDHVRELHAKRKKLPMWTIDKARANATQLTYAPVVPRALGRRVFKNFDL
                     AEIAKYLDWGPFFQTWDLAGSFPAILDDEVVGVEARKVYADGQAMLKKIIEGRWLTAN
                     GVMGLFPANRVGDDIEFYTDETRSQVLMTWYGLRQQTEKQNDAEGRLRPSRCLADFVA
                     PKESGIADYAGLFAVTAGIGSEKKEKEFLDALDDYSSIMFKGLADRLAEGFAECLHER
                     VRKDLWGYGADEALSNEDLIKEKYQGIRPAPGYPACPDHTAKIALFETLNTDEIGMQL
                     TESMAMFPASSVSGFYLAHPQATYFVVGQIGDDQVQDMAARRGMDVDELRRRLAPNLG
                     "
     regulatory      complement(9984..10061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            10309..>10686
                     /locus_tag="PK518_10635"
     CDS             10309..>10686
                     /locus_tag="PK518_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM49415.1"
                     /translation="MSTTHPLLLQPEGKTLELKRDLSSPQNLLKTLVAFANSAGGRLV
                     IGVDDARRVVGVSVDGVIASIAEFTGVDSVIHARPPASLPQHHRQRAGTTIKSPPEAA
                     VDTDQTHEIHPRRRHPPRQPAGRP"
BASE COUNT         1662 a         3671 c         3542 g         1811 t
ORIGIN      
        1 atttacaagc gcgtgcgcca tagccagacg cggccgctgc tgcaggtggc cgacccacag
       61 gcgcgcctgc gcgagctcta ccgcacacgc gacccgctgt accgcgaggc cgcgcactac
      121 gtgatcgaaa ccggccgccc cacggtgaac acgctggtga acatgatcgt gatgcagctg
      181 gagatggcgc cgggggcttg aggccggata ccgcgcgcgt cccgcatgac ctctgccccg
      241 cccatcctcc acggcccgga tcggcccgag ctgctgcgcg gcgaagtcct ggccgacctg
      301 ttcgaggcca cggccgcgcg cttgccgcac aagaccgcgc tcatcgccgg cggcgagcac
      361 ctgagctatg gcgcgctgaa cgccgccgcc gacctggccg cacaccgcct gatcttggcc
      421 ggcctgcggc ccggcgacat ggtcggcctg tggctgccgc gcgggctgga gctgctcatc
      481 tgccagctgg ccatcgccaa gactggcgcc gcctggctgc cgtttgacgc cgaggtgccg
      541 accgagcgca tcgccatctg cctggacgat gccaacgcca gcgcactatt gatttcggag
      601 ctatcggcgc ccgccatacg ggcgcaaacg ggtatttcgg cacaaatcct cacggcgcag
      661 gcggtgctgg cgccactgcc ggccggcgaa ccactgcgcc gccgcgcgga cgccgcaccc
      721 gagcacacgg cctacgtcat ctacaccagc ggctccaccg gcaagcccaa gggcatcgcc
      781 gtcacccagg ccagcatctg ccactttttg cgcagcgaga acgcgcgcct gggcgtgcgc
      841 gaggacgacc gcgtgtacca gggcttctcg gtggccttcg acatgagctt cgaggagatc
      901 tggatcagct acctggtggg cgccacgctg tggctggcgc ccaaggagat cgccggcgac
      961 cccgaggccc tgccgcgcgc gctgatcgaa aacgacatca cggtgctgca cgccgtgccc
     1021 acgctgctgg cgctgttcgc gcaggatgtg cctggcctgc gcatcatcaa cctgggcggc
     1081 gagatgtgcc cgcaggcgct ggtggaccag tgggccacgc ccggtcggca gatgttcaac
     1141 acctatggcc ccaccgaggc cacggtgtcg gccagcctgg ccgaactgca cgcgggcgag
     1201 ccggtgacga ttggcgaggc actgcccaac tatgggctgc tggtgatcga ggtgatcgag
     1261 ccggaggcca tcgtggacgg ccgcctgtca cctttgaagc tgctgccctt tggcgagacc
     1321 ggcgagctgt gcatcaccgg ccccggcgtg gccgccggct acctgggccg gcccgagctg
     1381 acggccgaga agttcctgcc caacccctgg gcgcgcaacg actgcgaggc gcgcctgtac
     1441 cgcaccggtg acctggcgcg cgtggagttg agggccgacg gccagccgca gatgcaatgc
     1501 ctgggccgcg ccgatgacca ggtcaaggtg cgcggctttc gcgtggagct gggcgagatc
     1561 gaggccgtgc tggcggcgca gcccggcgtg ggcaccacgg ccgtggtgct gcggcccgat
     1621 gcgggcatgg atcagctgat cgccttttac gtgcccaacg gccagccgcc cgaggccaag
     1681 accctgcgcg ccgcgctggc cgacaggctg ccgccctaca tggtgccggc gcgcttcgag
     1741 gacctggccg ccatgccgcg cctgtccagc ggcaagatag accgcaaggt gctgaaaagc
     1801 cagccgctgg ccgccgccgc gccggccgag ggtggcgatg agcccgagac gccggccgag
     1861 accgcgctat ttgccgcgct gcaggggctg tttcccggcc agccgctgcg ccgcgcgcag
     1921 gactttttca gcgacctggg cggccactcg ctgctggccg cgcgcctggt ctccaggctg
     1981 cggcaggacg cgtgctttgc ccgggccacc gtggccgaca tctacacgca ccgccgcctg
     2041 ggcgccatcg cccaggcgct gcaggccggc atggtcgatg cgcgggccgg cgcggccgag
     2101 cgccccttcc tcatccacgg ccgctggcgc cgctggcgct gcggcctggc ccagggccta
     2161 gcgattccgc tgctggtgct gctgaacatg gcgcagtggc tggccccctt ctttgcctac
     2221 cacttctaca ccggcgatgc gggggactcg attacccgcg ccgtggccat gtcggtgctg
     2281 gcgtttttgg ccagcacgct gctgggcttt ggcgtggcca ccacggggcg ctggctgctg
     2341 gccggccgcc tggcaccggg cagctacccg ctgtggggcc tgacctacta ccgctggtgg
     2401 ctggccgatc ggctaaccga gaccgtgccc gtgtacctgc tcaacggctc ggcgctgtac
     2461 ccggcctggc tgcgcctgct gggcgcgcgc atcgggcccg aggtggtgct gggttccacc
     2521 accatgcgcg tgccgcacct ggtcagcatt ggcgcgggca ccagcatagg caacggcgtg
     2581 aatctggaaa atgcccgcgt cgagggcggc cggctgcacc tgggagccat agacattggc
     2641 gccaacgcct gcataggctc ctacgtggtc atcgaaggcg acacggtggt cggtgactgg
     2701 gctcatgtgg agggccagtc ggccctggcc gcgggccagc gcgtgccggc gcgccaggcc
     2761 tggcatggct cgccggcgcg ccagcgcggc gacttcgacc ccgccagcct gcccgcgcgc
     2821 ccggccgtgt ccgccgcgcg cagggccggc gaggtgacct tcttcgccct ggcggcgctg
     2881 gccgtggcgg tgctgttctt catgccggtg tttcccacct tcatgctcat cgactggctg
     2941 gacatccccg agatctccgt gcgcccgctg gtagacgacg gcagcatcag cgccttgcag
     3001 gccttcggcc tgcgcctggt gaagttcttc gtgctggcac tgccggccag cctggtgttc
     3061 atcctggtca cggtgctgct gtcggccggc atccgctggg ccttgctgcc gcgcatgcgc
     3121 gcgggcagct ggccggtgca cagcccgcgc tacctggcca agtggacggt gaacctgatc
     3181 caggaggcca gcctggcggt gctgcacggc atctacgcca ccgtgtattc ggccgcctgg
     3241 taccggctgc tgggtgcgcg cgtgggcaag gagaccgagc tgtccaccgc cctgggcgtg
     3301 gtacccgaca tgctgacgct gggcgacggc tgcttcatcg ccgacgccgt gatgctgggc
     3361 gacgagcata tcgacggcgg ctggatgacg gtgcagccca ccgtcgtgtc gcggcgcagc
     3421 ttcatcggca acggcgccta cgtgcccgac ggcaccaccg tgcccgagaa cgtgctcata
     3481 ggcgtgctca gcagcgtgcc gcgcaacgcc agcatgcagg acggcgacac ctggctgggc
     3541 gcgccgccgc tgcgcctgcc ggcacgcgag caggtggcgg gcttccccga gcacctgacg
     3601 tttgcgccct cgccctggcg caaggcggca cgcgggctgg tggaggcgtt tcgcatcgcc
     3661 gcgccccacg cgctggtgat cgccaccggc tacgccatcg tgctggatgc catgccgctg
     3721 gccgaggagg gccgctgggc cgccgtggcg ctggacctgg cggtgggcgg catgctcttc
     3781 ggcatggcca ccttcggctt cgtggcgctg ttcaaatggc tggccgtgcg ccgctacggg
     3841 cagcgcgccg cgcccatgtg gacgcccttc gtgtggctgt ccgaggccgc caccaacctc
     3901 tatgaaggcg tggccgtgcc ctggtttttg cgctacctgc gcggcacgcc ctggctcaac
     3961 gatgcgctca agctgctggg cgcagacatc ggccgcggtg tgttcctgga caccaccgac
     4021 atgaccgagt tcgactgcgt gcacatcggc gcgcacagcg agctcaacgc cctgtgctgc
     4081 ccgcagacgc atctgttcga ggaccgggtg atgaagatcg accatgtgcg cataggcagc
     4141 cgcgtcacgc tggggccacg ctgcaccgtg ctgtacggcg cccaggtggg cgatggcgcg
     4201 cagctgggcc cgctgacgct ggtgatgaag ggcgagcaga ttccggccgg cacgctctgg
     4261 cacggcctgc cggctgcgcc ttggcgctga gcacggcagc ttgcgcgcga ggctcgttcg
     4321 gtgcagtttt ttctccatcg cccccttggg ggagagggag aaacagccct aaggcactgc
     4381 ccatgctgcg caagacgcta tgaaattatg tgttcagacc taagcttgcc atgccccttc
     4441 gccggcgacc gcctggccgg cgcgccccag gcgcatgcgc tcgatacgcg cctggcacag
     4501 gcagtggcac ccgggcagga gcgcgatcat gcccgcgctc aggcccgcgc cgcgctgcgc
     4561 cggctgctgg ccgccgtact cggctgcgcg ccgcaggcgc tcagcgtgag cgacgcgcgt
     4621 ggaataccac cgcgcctggg ctggactacc ggcggccggt cggggctgga cgccatcggc
     4681 ctgtcgatca gccacgcgcc tggcctgtcg ctggtggcct ggtgccccaa gggcgcggtg
     4741 ggcgtcgatg tgcaggccgt gccgtacgcc accgatcgcg ccgagctgtg gcgcacggcg
     4801 gcgctgtttt ttccaccaga caggctggaa gcactggctg agcatgcgtc agacgctctt
     4861 tttagtgaag cgttcgcggg ccactgggca gcccacgagg cggcgctcaa atgcctgggc
     4921 cagggcctgg tggaatacac gcccacgctg gcggcgcggc tggccggggt gcgcacggcg
     4981 gccctcaccc tgccgggctg ggccgccgca gacgtggtgg ccgccatcgc ctggcgcccc
     5041 taaccgccat gctgttcaga tagcgatcga tgccatttgt gcgcaggagc cgttcactga
     5101 accgcactat tgatccggca agtaacggtt gttggctgtc atcatctcgg ccttcgccgg
     5161 gatgacggtc aatcctttgt tgccggggca ataaccgggt cacgacgcgg caatcacgcc
     5221 gcaggcgatg cgcgcgcccg agttgccggt gggctgcgtc ttatagtcgt ccgcgtcctt
     5281 gtgcaccacc agcgaccgcc cgacgatgct ggtcggcccg ggcgcgaccg tgacgcccgt
     5341 cacgctgaac tcggcccggg ccttgccgct ggcgtcggcc ttcagcgccg gcaggtcacc
     5401 gccatggtga tcggcgttct gcggcccatg gggcttgccc gtagggttga aatgcccgcc
     5461 cgcgctcatg gcgtcgggtg ccgagcagtc gcccttgtcg tggatgtgca ggccatgctc
     5521 ctgccccggc gtcaggccgc tgacctcgac gcgcaccagc acccggtcgc cctgctgcct
     5581 gaagttggcc tggccgctcg tggccgagcc gctgcgcggc tgcaagctgg tgccggcgcc
     5641 caggccctgg ccggccagat ggccgcaggc cgtgagcagc aggacgctgg cgcccagtgc
     5701 ggcgtgggaa agaaatcgca tggcaggtct ccttcggtgc ttcagtgtgt cgatgcaatg
     5761 ggtggcgcgg cgcaacctgc accggccaag cctattcttg ccgataccgg tgcgtgccgt
     5821 gccacggcta tcatttcgga gcagctctcc aagaaagccc aggccatgcc cgattccacc
     5881 ggcaattgcg acttcatcgc gctctccaac gactcttacc gcctgctgct cgacgaggtc
     5941 ggcgcctttg tctacaccac cgacatggtc ggccgctaca cctttgccaa ccgcatggtg
     6001 ctgcagctct tggggcaccc gctggactat gtggtcggca aggacatcag ccatttcttt
     6061 ggcgacaagg gcaacgaggc gctgcgcgag accgacgagc gcgtgctgcg cggcggcgag
     6121 accattgcgc gcgaagagag caacctgatc ctggccaccg gcgagctgcg cacctactgg
     6181 tccaccaaaa aacctctgcg cgacgccacg ggccagatca tcggcatgat cggcatctcg
     6241 cacgacatca ccgaaaaaaa acgcctggaa gacaagctgc gccggcaaaa ggagctgctg
     6301 gacgcgctgg tggacaacgt cgatgcgctg atctacatga aggacgccaa ccggcgcttt
     6361 ctctacgcca accggcgcat ggccgaggcg tttggctgct cggtggacca gatcatcggc
     6421 agcctggacg tagacctgat gcccgccgag gtggccgagc gcttctggca gcaggatcaa
     6481 aaaatcctcg ctaacggcca gcgccacgcc tgcgaggagt cgctggtcga tgccagcggc
     6541 cgcctgcgcc actactggag cgtgatcatc ccctgggccg gccccgacgg cacgccggcc
     6601 atcatcggcc tgagcaccga catcaccgag ctgcacgcgc tcaaggaaga gctgcaacgc
     6661 caggcccggg ccgacagcct gaccggcatc gccaaccggc gcagcttcta cgagaacgcg
     6721 gagctggaat ttgcacgcag ccgcctgcag gggcaggcgc tgagcctgat caccatcgac
     6781 atcgacttct tcaagaagat caacgacacc ctgggccacc ccatgggcga ccgcgtgctg
     6841 caggagtttg tcgcctgctg ccaggacgcg ctgcgcaacg acggcctgct ggcgcgcacc
     6901 ggcggcgagg agttctgcat cctgctgccg ggcacggatc tggacacggc ccgacatatt
     6961 gccgagcgca tacgcgagca gacgcaggcg cggcgcatag cagtgccggc gcaggagctg
     7021 cgcatcagcg ccagctttgg cgtggccggc ctgtgcgata ccgacgagcg cttcgagacc
     7081 ctgtattccc gtgccgacct ggccctgtac gccgccaagc aacaggggcg caaccgcacc
     7141 agcctgctac gctcgccggc gcaggagggc gtgctggcga cacccgttgc agactagccg
     7201 aggtttggcg ccaggcgccg gcgcagctcg tccacgtcca tgccgcggcg cgcggccatg
     7261 tcctgcacct ggtcgtcgcc gatctggccg acgacgaagt aggtagcctg cgggtgggcc
     7321 aggtagaagc cgctgacgct ggacgccggg aacatggcca tgctctcggt cagctgcatg
     7381 ccgatttcgt cggtgttcag ggtctcgaac aacgctattt ttgccgtgtg atccgggcag
     7441 gccgggtagc cgggcgcggg gcgtatgccc tggtattttt ccttgatcag gtcttcgttg
     7501 ctcaaggcct catccgcgcc gtagccccac aaatccttgc gcacgcgctc gtgcaggcat
     7561 tcggcaaagc cctcggccag gcggtcggcc aagcccttga acatgatgga gctgtagtcg
     7621 tcgagcgcgt cgagaaactc tttctctttc ttctcgctgc caatgccggc ggtcacggcg
     7681 aacagccccg cgtagtcggc aatgcccgac tccttgggcg cgacgaaatc cgccaggcag
     7741 cggctggggc gcagccggcc ctcggcgtcg ttttgcttct cggtctgctg gcgcaggcca
     7801 taccaggtca tcaagacctg gctgcgcgtc tcatcggtgt agaactcgat gtcgtcgccc
     7861 acgcggttgg ccgggaagag gcccatcaca ccattggccg tcagccagcg gccttcgatg
     7921 atcttcttga gcatggcctg gccgtctgcg tagaccttgc gcgcctcgac cccgaccacc
     7981 tcgtcgtcga ggatggcggg aaagctcccg gccaggtccc aggtctggaa gaacgggccc
     8041 cagtccaggt atttggcgat ctcggccagg tcgaagttct tgaacacgcg ccggcccagc
     8101 gcacgcggca ccaccggggc gtaggtcagc tgcgtggcat tggcgcgcgc cttgtcaatc
     8161 gtccacatgg gcagcttctt gcgcttggcg tgcagctcgc gcacatggtc gtagtcggcg
     8221 gcgacctcgg ccagatacgc atccttgccc tcgcccagca ggctttgcgc cacgctcacg
     8281 ctgcgcgacg catcgggaac gtaaaccacg gggccgtcgt aattgggcgc aatcttcacg
     8341 gccgtgtgca cgcgcgagca ggtggcaccc ccgatgagca gcggtatttt cttgacgcgg
     8401 aaatgatcgt ccttgtgcat ctcggcggcg acgtactgca tctcctccag gctgggggtg
     8461 atgagccccg acaggccgac gatgtccgcc ccctcggcct tggcacgcgc cagaatttcg
     8521 tggcagggca ccatcacgcc catgttgatg acctcgaagt tattgcactg caagaccacg
     8581 gtgacgatgt tcttgccgat gtcgtgcacg tcgcccttga cggtggcaat gacgatcttg
     8641 cccttgctgg aaacgtccag gcccgccgcc tcttgctggc gcttttcttc ctcgatgtag
     8701 ggaatcaggt gcgccacggc ctgcttcatc acgcgcgccg atttcaccac ctgcggcaga
     8761 aacatcttgc ccgcaccgaa caggtcgccg acgatgttca tgccgtccat cagtgggcct
     8821 tcgatcacat gcagcgggcg cccgcccttg gccaccacga tgtcctggta ggcctcctcg
     8881 gtgtcttcga cgatgaagtc ggtgatgcca tgcaccagcg cgtgcgacag gcgctcgccc
     8941 acggttttgg ggtgctctgg cgtgccgcgc cactccagct tcttgccctc gtccttggcc
     9001 gcgcccttgg cggattcggc aatctccaag agcctttccg ctgcatccgg gcggcggttc
     9061 aagaccacgt cttccacgcg ctcgcgcagc tcgggttcga ggtcgtcgta cacgcccacc
     9121 atgccggcgt tgacgatacc catgtccatg cccgccgcaa tcgcgtggta caggaacacg
     9181 gtgtgaatcg cttcgcgcac cgggtcgttg ccacggaagc tgaacgacac gttggacacg
     9241 ccgcccgaga ccttggcgcc gggcaggttc tgcttgatcc agcgcacggc ctcgatgaaa
     9301 tcgaccgcgt agttggcgtg ctcctcaatg ccggtggcca cggcgaagat gttggggtcg
     9361 aagatgatgt cctcgggcgc aaagccgacc tcatcgacca gcacgcggta ggcgcgctcg
     9421 caaatctcgg tcttgcgcgc aaaggtgtcg gcctggcctt gttcatcaaa cgccatgacc
     9481 acggcggcgg cgccgtaacg cttgatcagc ttggcctgct gcttgaacgg ctccacgccc
     9541 tctttcatgg aaatcgagtt gacgatgccc ttgccctgca ggcagcgcag cccggcctcg
     9601 atcacctccc acttggacga gtccaccatc accggcacgc gggcgatgtc gggctcgctg
     9661 gcgatcaggt tcaaaaagcg caccatggcc gccttgctgt cgagcatggc ctcgtccatg
     9721 ttcacgtcga tgacctgggc gccgttctcc acctgctggc gcgccaccga cagggcttcc
     9781 tcgtactgct cgttgaggat catgcgcgcg aaggccttgg agccggtgac gttggtgcgc
     9841 tcgccgacgt tgacgaacag gctatcggcg ccaatgaaca ccggctccag gccggagagt
     9901 ttcatggggg ggacaggggt gggggcagca gcggtcatgg ctcacctgtg caatcggttg
     9961 gcggggaaaa gcaaagagac aaggacaggt gagcgtcgtt gcggcccaga gcggccccaa
    10021 gcctgacggg tggcgcaccc gctgcaacgc tccttggcaa gggctggatt ttaccggcct
    10081 ggtatacggc tgctttatcg gaataaaaat aggccgtaat gtttatgtga caaccgtcta
    10141 acgctataaa acaaatagct tcaaagcatg taggagggtg tccctggagg cctgtgccgc
    10201 gggttgatga gcaacaagct tgccaggccg gaagtaaaca aatgtactga cacaatcggc
    10261 ggcagtgctt ttttattccg aacccgctgc cacgatgccc gaggccgtat gagtaccacc
    10321 catcccctgc tgctacagcc cgagggcaag acgctggagc tcaagcgtga tctgtcctcg
    10381 ccgcagaacc tgctcaaaac cctggtggcc tttgccaaca gtgcgggcgg gcgcctggtc
    10441 atcggggtcg atgatgcgcg gcgagtcgtg ggtgtatcgg ttgacggcgt catagcctcg
    10501 attgcggaat ttacgggcgt tgattccgtg atccacgccc gccccccagc aagcttgccg
    10561 cagcaccacc gccagcgcgc tggcaccaca atcaaatcac cgcctgaagc ggctgtcgac
    10621 actgaccaaa cccatgaaat tcatccacgc cgccgacatc cacctcgaca gcccgctggt
    10681 cggcct
//
LOCUS       DAOMTQ010000105        10605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1211_length_10605_cov_26.014313, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000105 DAOMTQ010000000
VERSION     DAOMTQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_10605_cov_26.014313"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="groEL"
                     /locus_tag="PK518_10640"
     CDS             <1..777
                     /gene="groEL"
                     /locus_tag="PK518_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRM49416.1"
                     /translation="EDIAILTGGKVIAEEVGLSLEKVTLADLGQAKTIEVGKENTTII
                     DGAGNADDIQARVKQIRIQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKE
                     KKARVEDALHATRAAVEEGIVAGGGVALLRARQAVGEVKGENADQDAGVKLIMKAIEA
                     PLREIVNNAGGEASVVVNAVLAGKGNYGFNAANDSYGDMLEMGILDPTKVTRTALQNA
                     ASVASLLLTTEAMVAEAPKDDAPAGMPDMGGMGGMGGMGM"
     gene            complement(895..1809)
                     /locus_tag="PK518_10645"
     CDS             complement(895..1809)
                     /locus_tag="PK518_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49417.1"
                     /translation="MDEHRLKCFVAVYEQGSVSAAATRLHMTQPPLSMLLRKLEGELG
                     VGLFDRSGHRLAPTATGELFYLRAKALLAQLQTMRRELREAEQGARGTVHIGCATAAS
                     LFVMPAVMEDLAACGLDITVDVQEGEAGHMLQRLRERGLDLIISRSEYRAPELETHAI
                     MDEPLHVALPPAHPLAGGARVRLADLRHERFLLHRSPLGTGISDMVLRACQQAGFVPN
                     VVYWGGETLPMLLMAQRGLGVAFAPQSFGQLAGQGLPRLLPLDGTELRTHLNVAWPRQ
                     LGLTPVAGRVRDLILARFAARDPDPGAD"
     gene            1926..2918
                     /locus_tag="PK518_10650"
     CDS             1926..2918
                     /locus_tag="PK518_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49418.1"
                     /translation="MQRIRRHIAALALLTCIAPMLGMTAAQAQTDWPKRNMKVVVPYP
                     AGGSADAMGRMVANKLAKAFPKVSVVVENIPGGATVPGALAVLRDAADGHTLFMASDG
                     TLNINRWLLKDVRYDADRDFTPVTVLNSYPHWLIVNPQGPYKSLDDLVKAIRAKPGKV
                     SISINTIGGSAHLALDNWRRVNDLDFAIVPYRGSPPAVTDLIGGHTDAHMDVVGSSAA
                     FARSARVLPVAVLQGTPIHEFPKVPTQDPKDPKTLVVQSNLSVVMRSGTPQDVVSRIY
                     AILQAGVKDSDFAKTLDTLNLDPVLLEPAQAKTFLQQETQRYGALVEKSGLEKQ"
     gene            2932..4458
                     /locus_tag="PK518_10655"
     CDS             2932..4458
                     /locus_tag="PK518_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRM49419.1"
                     /translation="MKFVIALIRHETNTFSPIPTVLSDFRRGTGEGPAYGEAARLACE
                     GTKNAATAYLDMAREMGAEVDFAVYASAVPSGVVTREAFEHLCDAVVRSASQGCDAVL
                     LDLHGAMVVDGYPDAEGELLRRLRECTPAGLPIGVSLDFHANFSSELIRNATVIAGYC
                     TYPHVDIYETGERVARSIRAKLEGRSDPVLLWRRLPMLTHMLRQTPASQPMKDIMDRA
                     MQAEQSDEVCNASIFGGFPLSDIPCAGLSVVIVAERDRLQQGQKLLDELCDLAWSRRA
                     DFVFESGSVPESIAHAKTLKDGPVLLIDHGDNCGAGGVTDVMDVLEEVLKQGLQDVVA
                     GPFWDPATVATLFEHGVGAEVTVDVGGKTDMPALELKGRPLRLSGVVERLTDGEYTVT
                     GPMFTGVRQSLGRTAVLRVGSVRIFICERPQEPYDAGVFTHAGVDPAKAHYVLLKSRQ
                     HFRAGFGPFARHVVLVSGPGVCSSDYGLFPFKQLNRPMYPLDADATMADGESWIPAAA
                     "
     gene            4488..5216
                     /locus_tag="PK518_10660"
     CDS             4488..5216
                     /locus_tag="PK518_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRM49420.1"
                     /translation="MLYPAAVVLLGYVVLGITGFGSALVIVPLLAWRWPLTEVVPLVL
                     LLDVPASLLLGGLNLRHVNLAELRRLLPGMVAGALAGLWLSGQLPSRWPLLALGLYVA
                     AVGARALRSAPPQPRPLAAPWGHAAGGAIGLVEMLFGTAGPLVVAWLSRRLPDVLALR
                     ANTPIVITMSACAVLLTMGAAGRLSHAELWWRWLMLIVLAALGVLLGHHWARRVSAAS
                     LRKLICGLLVISGLSLVAHAVQLR"
     gene            5323..6306
                     /locus_tag="PK518_10665"
     CDS             5323..6306
                     /locus_tag="PK518_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRM49421.1"
                     /translation="MFHSSITKFAAVAFGAALLAQGASAQNQAHTPVKIIIGFPAGGA
                     LDNLARAMAEKLRTELGRTVIVDNKPGASTHIALMTVKRAPADGATILISPAPPFVTH
                     PLTYDKLQFDPDKDLIPVAHLADTPLVATTSAASPYSSMREYLEWVKKNPKDTGIGMV
                     SMGGVLHFGVLQLNQERGLSLMPVAYKGAPPMLTDEIGGVLPVGMDTVASASELEKAG
                     KIKYLGVPGMERTKLLPNVPTFKEQGVPGFEQASSWYAAYVPTGTPAAVVSQLEKAMI
                     HIVQDSAFAAKMAQGGMVTTGRPGAEVTSLIKSQREASRPIVEKSGYRATQ"
     gene            complement(6367..7359)
                     /locus_tag="PK518_10670"
     CDS             complement(6367..7359)
                     /locus_tag="PK518_10670"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRM49422.1"
                     /translation="MKIVVLGAGAWGTALAVSAAAHAGGNAVTLWARDEQQAAAMQAA
                     RCNQRYLPGLAFAPALQVVAGDARALAASAELIVIGTPMAALRGMLQLLKDSQAPIAW
                     LCKGFEAGTGLMAHEVCAEVAPQLASGVLSGPSFAQEVARAQPTALVAASRHAPVREA
                     LVQAFHGPSLRVYASEDIVGVEVGGAVKNVLAIATGLCDGLQLGLNARAALITRGLAE
                     MTRLGLALGARTDTFMGLSGLGDLVLTATGDLSRNRRVGLLLADGKSLQQAVDSLGHV
                     AEGVYSARTVVQRARAAGVEMPIAECVVALLDGRLHAADAVARLMEREPTVERR"
     gene            complement(7409..7867)
                     /gene="secB"
                     /locus_tag="PK518_10675"
     CDS             complement(7409..7867)
                     /gene="secB"
                     /locus_tag="PK518_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRM49423.1"
                     /translation="MSTEEATPVFQIQRVYLKDLSLEQPNSPAILLEQAQPSVDIQLG
                     VQATPVAEGIFEVAVTATVQTKIEDKTVFLVEAKQAGIFEIRNVPEEQMGAIVGIACP
                     QIIYPYLRGNVADTITRAGFPPVHLAEINFQAMYEQQQAAAAEAGNGTVQ"
     gene            complement(7961..8218)
                     /gene="grxC"
                     /locus_tag="PK518_10680"
     CDS             complement(7961..8218)
                     /gene="grxC"
                     /locus_tag="PK518_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HRM49424.1"
                     /translation="MQAVKMYTTAVCPYCTRAKQLLKARGVEQIEEVRIDTDPAERQQ
                     MMDITGRRTVPQIFIGDTHVGGFDDLAVLDQRGGLMPLLGA"
     gene            complement(8272..8676)
                     /locus_tag="PK518_10685"
     CDS             complement(8272..8676)
                     /locus_tag="PK518_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM49425.1"
                     /translation="MNFILDNWYLIVLALASGAMLLLPAVKGAAGGLSAAQAVQLINR
                     EKAVVIDVCEPAEFAAGHVGGAKNIPLAQLQERLPQVAKNKAVPVIMVCAKGGRAARA
                     VGIAKGLGYDKAQTLAGGLAAWREASMPVEKA"
     gene            8749..9492
                     /gene="gpmA"
                     /locus_tag="PK518_10690"
     CDS             8749..9492
                     /gene="gpmA"
                     /locus_tag="PK518_10690"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRM49426.1"
                     /translation="MHKLVLIRHGESTWNLENRFTGWTDVDLTTTGIEQAKNSGRLLK
                     AEGYEFDLAYTSVLKRATRTLWHCLDEMDRTWLPVQHSWRLNERHYGALQGLNKAETA
                     KQYGDEQVLVWRRSYDTPPPALEASDPRCERGDLRYAGLAPEQVPLTECLKDTVERVL
                     PFWNDTVAPTIRSGKRVVIAAHGNSIRALVKYLDNISDADIVGLNIPNGIPLVYELDA
                     DLKPLRHYYLGDAEAAAKAAAAVAAQGKA"
     gene            9591..>10605
                     /locus_tag="PK518_10695"
     CDS             9591..>10605
                     /locus_tag="PK518_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRM49427.1"
                     /translation="MGHKLKIAGWVSVGIVAGALTTVSLQTVARGAMTPLPLEEIQQL
                     SAVFGLIKTDYVEPVDDKKLITDAIGGMVSSLDPHSQYFDKKSFKEFREGTTGRFVGV
                     GIEITQEDGLIKIVSPIEGSPADRAGLKTNDLITKIDDTAVKGLTLNEAVKRMRGEPN
                     TKVTLTILRKDESRSFPVTITREEIKTQSVKAKVIEPGYAWIRLSQFQERTVDDFVRK
                     LEEIYKQEPRLKGLVLDLRNDPGGLLDAAVAVSAAFLPENVTVVSTNGQLAESKATFK
                     ASPEFYQRRGSGDPLKRLPAALKNVPLVVLVNEGSASASEIVAGALQDHKRATIMGSQ
                     TFGK"
BASE COUNT         1867 a         3612 c         3496 g         1630 t
ORIGIN      
        1 gaagacatcg ccatcctgac gggcggcaag gtcatcgctg aagaagtggg cctgagcctg
       61 gagaaggtga cgctggccga cctgggccag gccaagacca tcgaagtggg caaggaaaac
      121 accaccatca tcgacggcgc cggcaatgcc gacgacatcc aggcccgcgt caagcagatc
      181 cgcatccaga tcgaagaagc caccagcgac tacgaccgcg agaagctgca agagcgcgtg
      241 gccaagctgg ccggcggcgt ggccgtgatc aaggttggcg ctgccaccga ggtcgagatg
      301 aaggaaaaga aggcccgcgt tgaagacgcc ctgcacgcca cgcgcgctgc ggtggaagaa
      361 ggcatcgtgg ccggtggcgg cgtggccctg ctgcgcgccc gccaagcggt gggcgaggtc
      421 aagggtgaga acgccgacca ggacgccggc gtgaagctga tcatgaaggc catcgaggcc
      481 cccctgcgcg agatcgtcaa caacgccggc ggcgaagcct cggtggtggt caacgccgtg
      541 ctggccggca agggcaacta cggcttcaac gccgccaacg acagctacgg cgacatgctg
      601 gagatgggca ttctggaccc caccaaggtg acgcgcacgg cgctgcagaa cgccgcctcc
      661 gtggcctcgc tgctgctgac gaccgaggcc atggtggccg aggctccgaa ggacgatgcc
      721 cccgccggca tgcccgacat gggcggcatg ggtggcatgg gcggcatggg catgtaagca
      781 gccccggccg ctggcgcccc ccttcaccgg cggggtgcca tgcaccacga ccaaggcccg
      841 caggttttgc ctgcgggctt ttttttggcc ttggccgatc cggttgttgc ggcgtcaatc
      901 cgctccagga tcgggatctc gggcagcaaa gcgcgccagg atcagatccc gcacccggcc
      961 cgccaccggc gtcagcccaa gctggcgtgg ccaggcgacg ttcaagtggg tgcgcagctc
     1021 ggtgccatcg agcggcagca ggcgcggcag cccctggccc gccagttgcc cgaagctctg
     1081 cggcgcaaac gccacgccca ggccgcgttg cgccatcaag agcatgggca gggtctcgcc
     1141 gccccaatac accacgttgg gcacaaagcc cgcctgctgg caggcacgca gcaccatgtc
     1201 ggaaatgccc gtgcccaggg gcgagcggtg cagcagaaag cgctcgtggc gcaggtcggc
     1261 caggcgcacc cgcgcgccgc ccgccagggg gtgggcaggg ggcagggcca catgcagggg
     1321 ctcgtccatg atggcgtgcg tctccagctc gggcgcccgg tactcgctgc gcgagatgat
     1381 cagatccagg ccgcgctcgc gcaggcgctg cagcatgtgg ccggcctcgc cctcctgcac
     1441 gtccaccgtg atgtccagcc cacaggcagc caggtcctcc atcaccgccg gcatgacgaa
     1501 caggctggcc gccgtggcac agccgatgtg caccgtgccg cgcgcgccct gctcggcctc
     1561 gcgcagttcg cggcgcatgg tctgcagctg cgccagcagc gccttggcgc gcaggtagaa
     1621 cagctcgccc gtggccgtgg gcgccaggcg gtgaccgctg cggtcaaaca ggcccacgcc
     1681 cagctcgccc tccagcttgc gcagcagcat gctcagcggc ggctgggtca tgtgcaatcg
     1741 cgtcgcggcg gcagacacgc tgccctgctc gtagacggcg acaaagcatt tcaggcggtg
     1801 ctcatccata tgcttatcga ataaaaacag acgttatata tattagacag tggcttcttg
     1861 tcttcctaca ctggcttgca tcacggtaat gaaccgcgaa ccacagaccg ataggagaca
     1921 tgaatatgca acgcattcgc cgccacatcg ccgccctggc cctgctgacc tgcatcgcac
     1981 ccatgctggg catgacagcc gcccaggcgc agaccgactg gcccaaacgc aacatgaagg
     2041 tcgtcgtgcc ctacccggcc ggcggctccg ccgatgcgat ggggcgcatg gtggccaaca
     2101 agctggccaa ggccttcccc aaggtcagcg tggtggtgga gaacattccc ggcggcgcca
     2161 ccgtgcccgg tgcgctggcc gtgctgcgcg acgcggcgga cggccacacg ctgttcatgg
     2221 ccagcgatgg cacgctgaac atcaaccgct ggctgctcaa ggacgtgcgc tacgacgccg
     2281 accgcgactt cacgcccgtg accgtgctca acagctaccc gcactggctg atcgtcaatc
     2341 cccaggggcc ctacaagagc ctggacgacc tggtcaaggc catacgcgcc aagcccggca
     2401 aggtctccat cagcatcaac accataggcg gcagcgccca tctggcactg gacaactggc
     2461 gtcgggtgaa tgacctggat ttcgcgatcg tgccctaccg aggctcgccg ccggccgtga
     2521 cggatctgat cggcggccac accgacgcgc atatggacgt ggtgggatcc tcggcagcct
     2581 tcgcccgcag tgcgcgggtg ctgccggtgg ccgtgctgca gggcacgccc attcacgaat
     2641 tccccaaggt gccgacgcag gatccaaagg atccaaagac cctggtcgtg cagtccaacc
     2701 tctcggtggt catgcgcagc ggcacgccgc aggacgtggt gagccggatc tacgccatct
     2761 tgcaggcggg tgtgaaggac agcgatttcg ccaagaccct ggacacgctg aacctcgacc
     2821 cggtgctgct ggaacccgcc caggccaaga cctttttgca gcaggagacc cagcgctacg
     2881 gggccttggt cgagaagtcc ggcctggaaa aacagtaaaa aaggcattgc catgaaattc
     2941 gtcatcgccc tgatccgcca cgagaccaat accttctcgc ccatccccac cgtgctcagc
     3001 gactttcgcc gtggcacggg cgaggggccg gcctatggcg aggccgcgcg cctggcctgc
     3061 gagggcacaa agaacgcagc cacggcctac ctggacatgg cgcgcgagat gggcgccgag
     3121 gtcgactttg ccgtctacgc cagcgccgtg cccagcggcg tggtcacgcg cgaggccttc
     3181 gagcacctgt gcgacgcggt ggtgcgcagc gcctcgcagg gctgtgatgc ggtgctgctg
     3241 gatctgcatg gcgccatggt ggtcgatggc tacccggacg ccgagggcga gctgctgcgc
     3301 cgcctgcgcg agtgcacccc ggccggcctg ccaataggtg tgtcgctgga ctttcatgcc
     3361 aacttcagca gcgaattgat acgcaacgcg acggtaattg ccggctactg cacctatccg
     3421 catgtggata tctacgaaac cggcgagcgc gtggcgcgca gcatccgcgc caagctggag
     3481 ggccgcagcg acccggtgct gctgtggcgc cgcctgccca tgctgacgca catgctgcgc
     3541 cagacgcccg ccagccagcc catgaaggac atcatggacc gcgccatgca ggccgagcag
     3601 agtgatgagg tatgcaacgc ctccatcttc ggcggttttc cgctgtcgga catcccctgc
     3661 gctggtcttt ctgtcgtcat cgtcgccgaa cgtgatcgac tgcagcaggg ccagaagctg
     3721 cttgacgagc tgtgcgacct ggcctggtcg cgccgcgccg acttcgtctt cgagtcgggc
     3781 tcggtgccgg agtccattgc ccacgccaag accctcaagg acggaccggt gctgctcatc
     3841 gaccatggcg acaactgcgg cgcgggcggc gtgaccgacg tcatggacgt gctcgaagag
     3901 gtcttgaagc aaggcctgca ggatgtggtc gccggcccgt tctgggaccc ggccacggtg
     3961 gccacgctgt tcgagcacgg cgtgggcgcc gaggtcaccg tggacgtggg cggcaagacc
     4021 gacatgccgg cgctggaact gaagggccgg ccgctgcgcc tgtcgggcgt ggtggagcgc
     4081 ctgacggatg gcgaatacac cgtcaccggc cccatgttca cgggcgtgcg ccagagcctg
     4141 ggccgcaccg ccgtgctgcg cgtcggcagc gtgcgcatct tcatctgcga gcgcccgcag
     4201 gagccgtatg acgcgggcgt gttcacccat gcgggtgtcg atccggccaa ggcccactat
     4261 gtgctgctca agtcgcgcca gcatttccgc gccggcttcg ggccttttgc caggcatgtg
     4321 gtgctggtca gcgggccggg cgtatgcagc tcggactacg gcctgttccc gttcaagcag
     4381 ctgaaccgcc ccatgtaccc gctggatgcc gacgccacca tggcggatgg cgagagctgg
     4441 atacccgccg ctgcctgacc cacgccgcgt gcgccaccat gcaggaaatg ctctaccccg
     4501 ccgccgtggt actgctgggc tatgtggtgc tcggcatcac gggctttggc tcggccctgg
     4561 tcatcgtgcc gctgctggcc tggcgctggc ccctgaccga ggtcgtgccc ctggtgctgc
     4621 tgctggatgt gccggcatcg ctgctgctgg gtgggctgaa cctgcgccat gtgaacctgg
     4681 ccgagctgcg ccgcctgctg ccgggcatgg tggccggcgc attggcgggc ttgtggctgt
     4741 cgggccagct gcccagccgc tggccgctgc tggccctggg cctgtatgtg gcggccgtgg
     4801 gggcgcgcgc gctgcgcagc gcgccgccgc aaccgcggcc gctggcggcg ccctggggcc
     4861 atgcggccgg tggcgcgatt ggcctggtgg aaatgctgtt cggcaccgcc ggcccgctgg
     4921 tggtggcctg gctgagtcgg cgcctgcccg acgtgctggc gctgcgggcc aacacgccca
     4981 tcgtcatcac catgagcgcc tgcgccgtgc tgctgaccat gggcgcggcc gggcggctgt
     5041 cgcacgccga gctgtggtgg cgctggctga tgctgatcgt gctggccgcc ctgggtgtgc
     5101 tgctgggcca ccactgggcg cgccgcgtgt ccgcggccag cctgcgcaag ctgatctgcg
     5161 gcctcttggt catcagcggg ctgagcctgg tggcgcacgc cgtgcagctg cgctgaacct
     5221 gttatttcga aggagatgcc tttgccaccc caatacccgc gtttcacccc ataaccttca
     5281 acaaaatcac gtaaaaaccc gttcccgaag gagtcaggag acatgttcca ttcatccatc
     5341 accaagtttg ccgccgtcgc cttcggtgcc gcgctgctcg cccagggcgc cagcgcacag
     5401 aaccaggccc atacacccgt caagatcatc atcggctttc ctgccggcgg cgccctggac
     5461 aacctggcac gcgccatggc cgagaagctg cgtaccgaac tgggccgcac cgtcatcgtg
     5521 gacaacaagc ccggcgccag cacccatatc gcgctgatga ccgtcaagcg cgcgccggcc
     5581 gacggcgcca ccattctcat cagcccggcg ccgcccttcg tcacccaccc gctgacctac
     5641 gacaagctgc agttcgaccc ggacaaggat ctgatcccgg tggcgcatct ggcggatacg
     5701 ccgctggtgg ccaccacctc ggccgccagc ccgtattcgt cgatgcgcga atacctggag
     5761 tgggtgaaga aaaaccccaa ggacaccggc atcggcatgg tgtccatggg cggtgtgctg
     5821 catttcggcg tgctgcagct gaaccaggag cgcggcctga gcctgatgcc cgtggcctac
     5881 aagggcgccc cgcccatgct gaccgatgag atcggcggcg tgctgcccgt gggcatggac
     5941 acggtggcct cggccagcga gctggagaag gccggaaaga tcaagtacct gggcgtgccc
     6001 ggcatggagc gcaccaagct gctgcccaac gtgcccacct tcaaggagca gggcgtgccg
     6061 gggtttgaac aggcatcgag ctggtacgcc gcctatgtgc ccacgggcac gcccgcggcg
     6121 gtcgtgagcc agcttgaaaa ggccatgatc cacatcgtgc aagactcggc ctttgccgcc
     6181 aagatggccc agggcggcat ggtgaccacg ggccggccgg gcgcagaggt caccagcctg
     6241 atcaagtcac agcgcgaggc atcgcgcccc atcgtcgaaa aatcgggcta tcgcgcgacg
     6301 caataaccac gtttgagcgc agatgtcacc aatataacga caactgccaa atgcgcaggg
     6361 gcggcttcag cgccgctcca ccgtcggctc gcgctccatc aggcgcgcca cggcgtcggc
     6421 agcgtgcagc cggccgtcga gcagcgccac cacgcattcg gcgatcggca tctccacgcc
     6481 ggccgcgcgc gcgcgctgca ccaccgtgcg tgcgctgtac acgccctcgg ccacatggcc
     6541 cagcgagtcc accgcctgtt gcaggctctt gccatccgcc agcagcaggc ccacgcggcg
     6601 gttgcgcgac aggtcaccgg tggcggtgag gaccaggtcg cccaggccgg acaggcccat
     6661 gaaggtgtcg gtgcgcgcgc ccagggccag gcccaggcgc gtcatctcgg ccaggccgcg
     6721 cgtgatcagc gcggcgcgcg cgttcaggcc cagctgcaga ccgtcgcaca ggccggtggc
     6781 gatggccagc acgttcttca ccgcgccgcc cacctccacg ccgacgatgt cctcgctggc
     6841 atagacgcgc agcgacgggc catggaacgc ctgcaccagc gcttcgcgca cgggggcatg
     6901 gcggctggcc gccaccaggg ccgtgggctg ggcgcgcgcc acctcctggg caaagctggg
     6961 gccactgagc acaccgctgg ccagttgcgg cgccacctcg gcgcagacct catgggccat
     7021 caggccggtg ccggcctcaa aacccttgca cagccaggcg atgggggcct ggctgtcctt
     7081 gagcagttgc agcatgccgc gcaaggccgc catcggcgtg ccgatgacga tcagctcggc
     7141 gctggccgcc agcgcgcggg catcgccggc caccacctgc agcgccggcg caaatgccag
     7201 gccgggcagg tagcgctgat tgcagcgcgc ggcctgcatg gcagccgcct gttgctcgtc
     7261 gcgcgcccac agcgtgaccg cgttgccgcc ggcatgggcg gccgcgctca cagccagggc
     7321 cgtgccccag gcgcccgccc caagtactac tattttcata gcttgcagcg cttactggac
     7381 ggacgttaca accctaaaag atgaaaaatt actgaaccgt gccgttgccg gcctcggcgg
     7441 cggcggcctg ctgctgctcg tacatggcct ggaagttgat ctcggccagg tgcacgggcg
     7501 ggaagccggc gcgcgtaatg gtgtcggcca cattgccgcg caggtagggg tagatgatct
     7561 gcgggcaggc gatgccgacg atggcgccca tttgctcctc gggcacgttg cggatctcga
     7621 agatgccggc ctgcttggcc tcgaccagga acacggtctt gtcctcgatc ttggtctgca
     7681 cggtggccgt cacggcaacc tcaaaaatgc cttcggccac gggcgtggcc tgcacgccca
     7741 gctggatatc gacgctgggc tgcgcctgct ccagcaggat ggcgggggaa ttgggctgct
     7801 ccagcgacaa atccttcagg tagacgcgct ggatctggaa tacgggggtg gcttcttcgg
     7861 tggacatggc ttggggttcc gttggtcaaa acaaagcccg cccgggagac gctcccgcag
     7921 cgggcacttg gggctgcatt atgcaacctc gatcaggggg tcatgcgccc agcaggggca
     7981 tcaggccgcc gcgctggtcg agcacggcca ggtcgtcaaa gccgcccaca tgggtgtcac
     8041 caatgaagat ctgcggcacg gtgcgccggc cggtgatgtc catcatctgc tggcgctccg
     8101 ccgggtcggt gtctatgcgc acctcctcga tctgctccac gccgcgcgcc ttgagcagct
     8161 gcttggcacg ggtgcagtag gggcagacgg cggtggtgta catcttcacg gcttgcatga
     8221 cagggcaatc tttcagaaaa atgcagaaat atgaatgcag ataaaaccag atcaggcctt
     8281 ctcgaccggc atgctggctt cgcgccaggc ggccaggcca ccggccaggg tctgcgcctt
     8341 gtcgtagccc agacccttgg caatgcccac ggcgcgcgcc gcccggccgc ccttggcgca
     8401 gaccatgatc accggcacgg ccttgttctt ggccacctgc ggcagacgct cctgcagttg
     8461 cgccaacgga atgttcttgg cgccgcccac atggccggcg gcgaactcgg cgggttcgca
     8521 cacgtcgatg accaccgcct tctcgcggtt gatgagctgc acggcctgcg ccgcgctcag
     8581 gcccccggcc gcgcccttga cagcaggcag cagcagcatg gcgcccgaag ccagcgcgag
     8641 gacgatcaaa taccagttgt cgaggatgaa gttcacgagg gtccttaggg cgaaaaaaac
     8701 cgctcattct aaaataccgg gttttgactt gcacgaccca gggacaccat gcacaaactc
     8761 gttctgattc gccacggcga atccacgtgg aatctcgaaa accgcttcac cggctggacc
     8821 gacgtggatc tgaccaccac cggcatcgag caggccaaga actccggccg cctgctcaag
     8881 gccgagggct acgagtttga cctggcctac accagcgtcc tgaaacgcgc cacgcgcacg
     8941 ctgtggcatt gcctggacga gatggaccgc acctggctgc cggtgcagca cagctggcgc
     9001 ctgaacgagc gccactatgg cgcgctgcag ggcctgaaca aggcggagac ggccaaacag
     9061 tacggcgacg agcaggtgct ggtctggcgc cgcagctacg acaccccgcc gccggccctg
     9121 gaagccagcg acccgcgctg cgagcgcggc gacctgcgct acgccggcct ggcccccgag
     9181 caggtgccgc tgaccgaatg cctgaaggac acggtggaac gcgtgctgcc attctggaac
     9241 gacaccgtcg ccccgaccat ccgctcgggc aagcgcgtgg tgatcgccgc ccatggcaac
     9301 tcgatacgcg cgctggtgaa gtacctggac aacatctccg acgccgacat cgtgggcctg
     9361 aacatcccca acggcatccc tctggtgtat gagctggacg ccgacctgaa gcccctgcgc
     9421 cattactacc tgggcgacgc cgaggccgcg gccaaggccg cagccgcggt ggccgcgcaa
     9481 ggcaaggcct gaatccgaca gggcaaaaaa catgcctggc cagggaactg cccgggcttg
     9541 cacccttcca aggtgtatat tgaatcgcta gagacgcggt agaggtgcat atgggccaca
     9601 aactgaaaat tgcgggatgg gtatcggtcg gcatcgtggc tggcgcactg accacggtgt
     9661 cgctgcagac cgtggcgcgc ggcgccatga cgccgctgcc actggaagag atacagcagc
     9721 tgtcggcggt gttcggcctg atcaagaccg actatgtgga gccggtcgat gacaaaaagc
     9781 tcatcaccga cgccattggc ggcatggtct ccagcctgga tccgcactcg caatactttg
     9841 acaagaagtc cttcaaggaa tttcgcgagg gcacgaccgg ccgcttcgtc ggcgtgggca
     9901 tagaaatcac gcaggaagac gggctgatca agatcgtctc acccatcgag ggttcgcccg
     9961 ccgaccgcgc cgggctcaag accaacgacc tgatcacgaa gattgacgac acggccgtca
    10021 agggcctgac cctcaacgag gccgtcaagc gcatgcgcgg cgagcccaac accaaggtca
    10081 cgctgaccat tctgcgcaag gacgaaagcc gcagcttccc cgtgaccatc acgcgcgagg
    10141 agatcaagac ccagtcggtc aaggccaagg tgatagagcc cggctatgcg tggatccgcc
    10201 tgtcgcagtt ccaggagcgc acggtggacg actttgtgcg caagctcgaa gagatctaca
    10261 agcaggagcc gcgcctgaag ggcctggtgc tggatctgcg caacgacccg ggcggcctgc
    10321 tggacgccgc cgtggccgtg tccgccgcct tcctgcccga gaacgtcacc gtggtctcca
    10381 ccaacggcca gctggccgag agcaaggcca ccttcaaggc ctcgcccgag ttctaccagc
    10441 gccgcggcag cggcgacccg ctcaagcgcc tgcccgcggc cctcaaaaac gtgcccctgg
    10501 tggtgctggt caacgagggc tcggcctcgg ccagcgagat cgtcgccggc gcgctgcagg
    10561 accacaagcg cgccaccatc atgggcagcc agacctttgg caagg
//
LOCUS       DAOMTQ010000106        10432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1254_length_10432_cov_28.652790, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000106 DAOMTQ010000000
VERSION     DAOMTQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_10432_cov_28.652790"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1286
                     /locus_tag="PK518_10700"
     CDS             3..1286
                     /locus_tag="PK518_10700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRM49428.1"
                     /translation="MKFIHAADIHLDSPLVGLSAYPDAPAERLRTATREAFERLVARA
                     IEEQVDFMVIAGDLYDGDWKDYHTGLFFVRQMGRLRQAGITVYLLYGNHDAESEVTRS
                     LQLPDNVQVFPARRAETFRIAALQVALHGQSFKHAATTDNLVPGYPAPVPGWLNIGVL
                     HTALEGNAQHASYAPCSPAELHAKGYQYWALGHVHAHWVEQGDVTIAYPGNLQGRHIR
                     ETGAHGALLVTAEGTQITRIDRLEVDVLRWHLLEIDASQADDLPDALRLVGQALDQLL
                     RQEPAHMVLAVRVRFAGASGAHAALLRSDQQLRHEVIAQAVALNPERLWIEKVIADTQ
                     PLPLAQAPASGAAANAFAELQDLARSAAHDADFVRALQTGWQAVLEKLPHEVLAQVPE
                     LAALRQQPEEALAAQLHDALALLGARTGAAADAGR"
     gene            1305..4811
                     /locus_tag="PK518_10705"
     CDS             1305..4811
                     /locus_tag="PK518_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM49429.1"
                     /translation="MRINEFKLIRYGKFTDRTLHLPARAGAQGADIHLIVGPNEAGKS
                     TLRQAIGDWLFGFPTRTPMGFVHPMPELRLGGCLQRAGEATANAVSLAFERAKGNKNT
                     VRTPDDDALPDTALAPWLASLQREAYERMYGLDHDRLVAGGADILAASDDIGRLLFQS
                     AAGIEHLGDTLQKLELEADALWAPRKAGSRLYYQARDDFEAAGKDLKQHLLRAKDWQA
                     QHEALQTTAAQLHEARQQHQGVAQQTSRLERIRRVAPLLQTLDAARQQRDQLLAQGNI
                     TLLDEDAGQRLAQARQQLALIQADMQRLAQEKTQLTDALAATQVNHDLLARAGDITEL
                     NERRLQFRAHGSDIAKRTQEVHAHWQRVQELAGALGWAADTEDAVRQRLPVAAVRARL
                     ARLLKQHGGVDQDLRQAQAQLAERQQQIAQLAQQLQALPAQTAHPGLAQALDDALPFA
                     SHDSTRRDGQQKASALADKIETALAAMGNWRQSPQALAGMLAPQAAAVQALQQRQRDE
                     ARQAQTSHEAAEAKAQDIAQAELALQQLVRQFHPVSLEQLQQARQARDSLWQAIKAAP
                     AQLPQQAGAFENHLAQADQLADTRLERARHEADRQASAQRLEQLQLELSQLQQRSAAA
                     QERAHGCAAEWQTLAAAGSLPALPLDLAGAWLQQRQTALALVQEQAELEQQTHASQQA
                     ADAAHRKLWAALHAGSDNSTSTPPSAPLAECLRQARALIKAADQAQGQRDTLSRQLAD
                     WQASLPTLQSAVDKAQQAWQQWQSRWQAALVDAAYPASAQPDSIEAEIDTLQDIDRLL
                     GSMRSTRSDRIDAMQADLDGLAISAQALAARLAPELATQPAPEIALALAQQLEGARQA
                     QERADQLQARLDKAEAELHAAHNKQQAIDAGLAPLLAAAGCSDLDSLASAIDRSDQRR
                     HIDQHIHTTAQELAHAADGYPEPELRAQASHADPDAVKAELHTLAQQSGALLQQIEAL
                     SSRHGSQQAALDALDGASHAAQAEARRQEAIATMADAVQRYTRLNNASRLLRWSIEKF
                     RQTRQGPMLAQASSIFRTLTQDQFDRLLVDAEGHSPRLIGVRSSGAPVDVTGMSEGTR
                     DQLYLALRLAALQLQTAQGQALPFIADDLFINFDDGRTLAGLKVLGELSRHLQIIVLT
                     HHEHLLPLAQQALGKDLNVVRL"
     gene            complement(5187..5561)
                     /locus_tag="PK518_10710"
     CDS             complement(5187..5561)
                     /locus_tag="PK518_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM49430.1"
                     /translation="MTKLIIKTGTEEDFFARGRRLAKAADRGKALEAVRTISFEDPAD
                     VMKLITETRLALFRAVKEAPGSITDIAARLQRDRSAVKRDLDELEQVGLVTIAEKVLP
                     GHGRMKEVRATAARVQLVAEVF"
     gene            complement(5558..5854)
                     /locus_tag="PK518_10715"
     CDS             complement(5558..5854)
                     /locus_tag="PK518_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HRM49431.1"
                     /translation="MPEVKIHDESHKIPDKRGNGRLRREVWIDDQGRITRYNLAYINH
                     NLHAGDNGRVVGYDNAHGFHHRHYFGAVEAIDFISYEDVEERFELDWTALKGRK"
     gene            6061..7209
                     /locus_tag="PK518_10720"
     CDS             6061..7209
                     /locus_tag="PK518_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49432.1"
                     /translation="MPPITTYTQLEDFGRIRLSRNFFMRDFLHSEIAVWHGLRNVPVN
                     PDLAVRNGRMLCEQLLEPLQTTFGRIHIRSGYRSPEVNDLGNRNNLNCASNEKNCARH
                     IWDQPDANGQHGAMACIVVPWLVDYMQKGSSWTAMAWWIHDHLPYASLYFFAKLGAFN
                     IGWHEAPERRIDSYAPPKGCLTRPGMANHGGTHASEYPGFPQLVRPAARSTGVSAVNA
                     APALPAAPATSIAAPTTPRAVAAPMAATPTTRTPAFTPPAFARNQPTMTTTFPASNGG
                     PIRYRAIHEKTLWRKAGGHASLDSAIHGANGAAALFRGKVRIDYATHGQPHYAVVWQE
                     GQGTGYVIKPDASQPQDIRVQSVPVQKLQAFEVAGQASAAQLEALFQP"
     gene            7251..7514
                     /locus_tag="PK518_10725"
     CDS             7251..7514
                     /locus_tag="PK518_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49433.1"
                     /translation="MRTTLDISDDVLLAAKELARRGKKPLGQVISELARKAFTLPDDH
                     SAAAGTAKNADNPLAALGIHPLPPCGVIVSNEHIDRLRDEEGV"
     gene            7516..7899
                     /locus_tag="PK518_10730"
                     /pseudo
     CDS             7516..7899
                     /locus_tag="PK518_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
     gene            complement(7963..8376)
                     /locus_tag="PK518_10735"
     CDS             complement(7963..8376)
                     /locus_tag="PK518_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRM49434.1"
                     /translation="MAIRYLLDTNICIYIAKHNPPAVRARFEQLSSDALAMSVVTLGE
                     LQHGAEKSQARDKALAVLQQLQAAIQVMPLAPTAGAHYGQIRAALERAGQPIGNNDLW
                     IAAHARAEGWVLVTNNEREFCRVPGLAVENWVAAL"
     gene            complement(8377..8607)
                     /gene="vapB"
                     /locus_tag="PK518_10740"
     CDS             complement(8377..8607)
                     /gene="vapB"
                     /locus_tag="PK518_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRM49435.1"
                     /translation="MTYARVFQSGNSQAVRLPKEFRFNAEQVEIFRQGNDIVLRERPA
                     SAAAIFDALASLPADFMQDGRGDALPQEREAF"
     gene            complement(8792..9217)
                     /locus_tag="PK518_10745"
     CDS             complement(8792..9217)
                     /locus_tag="PK518_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRM49436.1"
                     /translation="MYLLDTNVISELRTGKPQADARVLAWAASVPLAAQYVSVLTWLE
                     IDIGILRLQRRDAAQAQALRAWLDGVRALFAERSLPVDDAVVQCCAPLHVPDPAPPHD
                     ALIAATALQHGLTLVTRNVRDFVHMGVALFNPWEFVGQG"
     gene            complement(9217..9507)
                     /locus_tag="PK518_10750"
     CDS             complement(9217..9507)
                     /locus_tag="PK518_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRM49437.1"
                     /translation="MSIATMNSRTFARDAAAVKRAAQQGPVIITERGKPSLAVLKIED
                     YYRLTGQVGGESLLQAMRGVDAPSGGELPLPERPGAADITLRIPEFGEHEPR"
     gene            9682..9945
                     /locus_tag="PK518_10755"
     CDS             9682..9945
                     /locus_tag="PK518_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49438.1"
                     /translation="MTLPQAIAFGTAGDKLSRKITGTNEVSVTRSTIATGAGAALGAA
                     GAGALSVGAGAVGLTAVATVAAPVAVPVAIVAGGIGLLCSLFE"
     gene            9942..10250
                     /gene="csx16"
                     /locus_tag="PK518_10760"
     CDS             9942..10250
                     /gene="csx16"
                     /locus_tag="PK518_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx16"
                     /protein_id="HRM49439.1"
                     /translation="MTTWFVSRHPGALHWMRQHGPAYDQHVPHLDPAQVQPGDAVIGT
                     LPVNLAAQVCARGAAYWHLALGVPAQSRGRELSADELLALGATLQRFNVQSHKFKPAP
                     "
     gene            10247..>10432
                     /locus_tag="PK518_10765"
     CDS             10247..>10432
                     /locus_tag="PK518_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49440.1"
                     /translation="MTTTLHLCIATGQNAANFIPLKQLAAHTIWILETPDMQKQQSAK
                     ALSTALKSALPEAQIRTL"
BASE COUNT         1985 a         3770 c         3161 g         1516 t
ORIGIN      
        1 ccatgaaatt catccacgcc gccgacatcc acctcgacag cccgctggtc ggcctgtcgg
       61 cctatcccga cgcgccggcc gagcgcctgc gcacagccac gcgcgaggcc tttgagcggc
      121 tggtggcgcg cgccatcgag gagcaggtgg acttcatggt gattgccggc gatctgtacg
      181 acggcgattg gaaggactat cacaccggcc tgttcttcgt gcgccagatg gggcggctgc
      241 gccaggcggg tatcaccgtg tacctactct acggcaacca cgatgccgaa agcgaagtca
      301 cgcgcagtct gcaactgccg gacaacgtgc aggtgtttcc ggcacgccgc gccgagacct
      361 ttcgcatcgc cgcgctccag gtggcgctgc acggccagag cttcaagcac gccgccacca
      421 ccgacaacct ggtgcccggc taccccgcgc cggtgcccgg ctggctcaac atcggcgtgc
      481 tgcacaccgc gctggagggc aacgcccagc acgccagcta cgccccctgc tcgccggccg
      541 agctgcacgc caagggctac caatactggg cgctgggcca tgtccacgcg cactgggtgg
      601 aacagggcga cgtgaccatc gcctacccgg gcaacctgca ggggcgccac atccgcgaga
      661 ccggcgcgca tggcgcgctg ctcgtcaccg cggaagggac gcagatcacc cgcatcgatc
      721 gcctggaagt ggatgtgctg cgctggcacc tactggagat agacgccagc caggccgacg
      781 acctgcccga tgccctgcgc ctggtgggcc aggcgctgga tcagctgttg cgccaagaac
      841 cggcgcacat ggtgctggcc gtgcgcgtgc gctttgccgg cgcgtctggc gcgcacgcgg
      901 cgctgctgcg cagcgaccag cagctgcgcc acgaggtcat cgcccaggcc gtggcgctga
      961 accccgaacg cctgtggatt gagaaggtca tcgcagacac ccagcccctg ccgctggccc
     1021 aggccccggc cagcggcgct gccgccaacg ccttcgccga attgcaagac ctggcgcgca
     1081 gcgccgcgca cgatgcagac tttgtgcgcg ccttgcaaac cggctggcaa gccgtgctgg
     1141 aaaagctgcc gcacgaggtg ctggcgcagg tgcccgagct ggccgcgctg cgccagcagc
     1201 ccgaagaggc gctggccgcg caactgcatg acgcgctggc gctgctgggc gcacgcaccg
     1261 gcgcggcggc agacgccggc cgttgatgac agagaaggca cgccatgcgc atcaacgaat
     1321 tcaagctcat ccgctacggc aagttcaccg accgcacgct gcacctgccc gcccgggctg
     1381 gcgcgcaggg cgcggacatc cacctcatcg tcggccccaa cgaggccggc aaatccacgc
     1441 tgcgccaggc cattggcgac tggctgttcg gcttccccac gcgcacgccc atgggctttg
     1501 tgcaccccat gccggagctg cgcctgggcg gctgcttgca gcgcgccggc gaggccaccg
     1561 ccaacgcagt cagcctggcg ttcgagcgcg ccaagggcaa taaaaacacc gtgcgcacac
     1621 ccgacgatga cgcgctgccc gacaccgcgc tggcgccctg gctggccagc ctgcagcgcg
     1681 aagcctacga acgcatgtat gggctcgacc atgatcggct ggtggctggc ggcgccgaca
     1741 tccttgccgc ctccgacgac atcggccgcc tcctgttcca gtccgccgcc ggcatcgagc
     1801 atttgggcga tacgctacaa aagctggagc ttgaagccga tgccctgtgg gcgccgcgca
     1861 aggccggttc acgcctgtac taccaggcac gcgacgactt cgaggccgcc ggcaaagacc
     1921 tcaaacaaca cctgttgcgc gccaaggact ggcaggcgca gcacgaggcg ctgcaaacca
     1981 ccgccgcgca gctgcacgag gcccgccagc agcatcaggg cgtggcgcag cagaccagcc
     2041 ggctggagcg catccgccgc gtggcgccgc tgctgcaaac gctggacgcc gcacgccagc
     2101 aacgcgacca actgctggcc cagggcaaca tcaccctgct ggacgaagac gccggccaac
     2161 ggctggccca ggctcggcaa cagctggcgc tgatccaggc cgacatgcag cggctggcgc
     2221 aagaaaagac gcagctcacg gacgcgctgg ccgccacgca ggtcaaccac gacctgctgg
     2281 cgcgcgccgg cgacatcacc gagctcaacg aacgccggct gcagtttcgg gcgcacggca
     2341 gcgacatcgc caagcgcacg caagaagtcc acgcccactg gcagcgcgta caagagctgg
     2401 ccggcgccct gggctgggcg gccgacaccg aagacgcggt gcgccagcgc ctgccggtag
     2461 cggccgtgcg cgcgcgcctg gcgcgcctgc tcaagcagca cggcggcgtc gatcaagacc
     2521 tgcgccaggc ccaggcccaa ctggccgagc gccagcagca aatcgcccag cttgcgcagc
     2581 aactgcaagc cctgccggca caaaccgcgc accccggcct ggcccaggcg ctcgacgatg
     2641 cgctgccgtt tgccagccac gacagcaccc ggcgcgacgg gcagcaaaaa gccagcgccc
     2701 tggcggacaa gatcgagacc gccctggccg ccatgggcaa ctggcgccag agcccgcagg
     2761 ccttggccgg catgctggct ccgcaagccg ctgccgtgca ggccttgcaa cagcgccagc
     2821 gcgacgaagc ccgccaggcg caaacctcgc acgaggccgc cgaggccaag gcgcaagaca
     2881 tcgcccaggc cgaactggcg ctgcagcagc tggtgcgcca attccacccc gtgtcgctgg
     2941 agcaattgca gcaggcacgg caggcgcgcg acagcctgtg gcaggccatc aaggccgcgc
     3001 ccgcgcaact gccccagcaa gcaggcgcat ttgaaaacca cctggcgcag gccgatcagc
     3061 tggccgacac ccgcctggag cgtgcccggc acgaggccga ccgccaggcc agcgcccagc
     3121 gcctggagca gctgcaactc gaactcagcc agctgcagca acgcagcgcg gcggcgcagg
     3181 agcgcgccca cggctgcgcc gccgaatggc aaaccctggc cgctgccggc agcctgccgg
     3241 ccctgccgct ggacctggcc ggcgcctggc tgcaacagcg ccaaaccgcg ctcgccctgg
     3301 tgcaggaaca ggccgagctg gagcagcaaa cccacgccag ccagcaggcc gccgacgcgg
     3361 cacaccgcaa gctgtgggct gcgctgcacg ccggcagcga caacagcacc tcgacgcctc
     3421 cctccgcccc cctggccgaa tgcctgcgcc aggcgcgcgc cctcatcaag gcggcagacc
     3481 aggcccaggg gcagcgcgac accctgtccc gccagctggc cgactggcaa gcgagcctgc
     3541 ctaccctgca aagcgcggtg gacaaagccc agcaggcctg gcagcaatgg caaagccgct
     3601 ggcaggctgc gctggtggat gcggcctacc ccgccagcgc gcagcccgac agcatcgaag
     3661 ccgagatcga cacgctgcaa gacatcgacc ggctgctggg cagcatgcgc agtacccgca
     3721 gcgaccgcat cgacgccatg caggccgacc tcgacggcct ggccatcagt gcccaggcac
     3781 tggcggcgcg cctggccccg gagctggcca cccaacccgc gccggagatc gcgctggctc
     3841 tggcgcagca gctggaaggc gcccggcagg cccaggagcg cgccgaccag ctgcaggccc
     3901 ggctcgacaa ggccgaggca gaactccacg cagcacacaa caagcaacag gccatcgatg
     3961 ccggcctggc gcccctgctg gcggccgctg gctgcagcga cctggacagc ctggccagcg
     4021 ccatcgatcg ctcagaccag cgccggcaca tcgaccagca catccacacc accgcgcaag
     4081 aactggccca cgccgcagac ggctaccccg agcccgaact gcgcgcgcag gccagccacg
     4141 ccgaccccga cgccgtcaag gccgagctgc acaccctggc ccagcagtcg ggcgcgctgc
     4201 tgcagcagat cgaagccctg agcagccgcc acggcagcca gcaggcggcg ctggacgcgc
     4261 tcgacggcgc cagccatgcc gcccaggccg aggcgcggcg gcaagaggcc atcgccacca
     4321 tggccgatgc cgtgcagcgc tacacccggc tgaacaacgc ctcacggctg ctgcgctggt
     4381 ccatcgaaaa gtttcgccaa acccggcaag gccctatgct ggcgcaggcc tccagcatct
     4441 ttcgcacgct cacccaagac cagttcgacc gcctgctggt ggatgccgaa ggccacagcc
     4501 cgcgcctgat cggcgtgcgc agctcgggcg cgccggtgga cgtcaccggc atgagcgaag
     4561 gcacgcgcga ccagctctac ctggcgctgc gcctggcggc cctgcaactg caaaccgcgc
     4621 aaggccaggc cctgcccttc atcgccgacg acctgttcat caacttcgac gacggccgca
     4681 cgctggccgg cctgaaggtg ctgggcgagc tctcgcgcca cctgcaaatc atcgtgctca
     4741 cacaccacga gcacctgctg ccgctggcgc agcaggcgct gggcaaagat ctgaatgtgg
     4801 tgcggctgta gtggtgtggt gctaatcact ttgcttgatt agcaaggagt ctcagactcc
     4861 ttccccctct gggggaaggc agggatgggg gctgatgtga cggcgacgtg gcgacatcga
     4921 cgaacgcgtg gtgctgcccc tgtcaactca ctttgatagc aaacaacgct tatttggcaa
     4981 cggctttaac tccctcgccc ccttggggga gagggtgggg gtgagggggt gatcgggtgc
     5041 agcgcccgct tttacgaccc tcaccccacc ctctcccgcc aacgggagag ggagaaacag
     5101 ccgcaaagcg ttgctgatgt tgcatgacgc actataaaaa tgtgtgcata ccgagggggt
     5161 gaggggcgtt ggcggcaggt acacagtcag aaaacctcag ccaccaattg aacccgggcc
     5221 gcagtggcgc gcacttcctt catgcgtcca tgtccaggca gcaccttttc cgcaatggtc
     5281 accagcccga cttgctccaa ctcatccagg tcacgcttga ctgcgctgcg gtctcgttgc
     5341 agtcgcgcag caatatccgt aatcgaccca ggcgcctcct tgaccgctcg aaacaaggcc
     5401 aagcgggtct cggtgatgag cttcatcacg tcggcagggt cttcaaagct gatggtgcgc
     5461 accgcttcga gggctttgcc acggtcagcc gccttggcca atcgacggcc tcgtgcgaag
     5521 aaatcttctt cggtgccggt cttaatgatg agcttggtca tttgcggcct ttcaatgcag
     5581 tccaatccag ttcaaagcgc tcttcaacgt cttcatagct gatgaagtcg atggcttcga
     5641 cagcgccaaa gtaatgccga tggtgaaagc catgggcgtt gtcatagcca accacccgcc
     5701 cgttgtctcc ggcatgaagg ttgtgattga tataggcgag gttgtagcgc gtaatccgcc
     5761 cctggtcatc aatccatact tcccgccgca ggcgcccatt tccacgcttg tcgggaatct
     5821 tgtgcgattc gtcgtgaatt tttacctcag gcatgattca tggtatcacc cctgacccgt
     5881 ctatggctcc gacttcagtg cacagcgaag gcccactgaa gctcctgatc ggagccgccc
     5941 caatccgtcg gcaagcttgc gacgctgccg ccacgcgccg agccgccaga caatggccgc
     6001 atggcattcc tgggttgatc caatgacaca gtgccacgat tcaccccaat ggagaacagc
     6061 atgcccccca tcaccaccta cacccaactc gaagacttcg gccgcattcg cctctcgcgc
     6121 aattttttca tgcgcgactt tctgcacagc gagattgccg tctggcatgg cctgcgcaat
     6181 gtgcccgtca accctgatct ggcggtgcgc aacggccgca tgctgtgcga gcagctgctg
     6241 gagccgctgc agaccacctt cgggcgcatc cacatccgct ctggctaccg ctcgcccgag
     6301 gtcaacgacc tgggcaaccg caacaacctc aactgcgcca gcaacgagaa gaactgcgcg
     6361 cgccacatct gggatcagcc cgatgccaac ggccagcacg gcgccatggc ctgcatcgtc
     6421 gtgccctggc tggtggacta catgcaaaaa ggcagctcct ggacagccat ggcctggtgg
     6481 attcacgacc acctgcccta cgccagcctg tacttctttg ccaagctggg cgccttcaac
     6541 attggctggc acgaagcgcc cgagcgccgt atcgacagct acgccccgcc caagggctgc
     6601 ctgactcgcc cgggcatggc caaccatggc ggcacgcatg ccagcgaata ccccgggttc
     6661 ccgcagctgg tgcgtcctgc cgccaggagc actggcgtca gcgccgtgaa cgccgcaccg
     6721 gcactacctg cggctcccgc aacctccatt gccgcaccca ccaccccgcg cgccgtggct
     6781 gcccccatgg ccgccacacc aacgacgcgc acgcccgcct tcaccccacc tgcctttgca
     6841 cgcaaccaac ccaccatgac caccaccttc cctgcatcca acggtggccc cattcgctac
     6901 cgcgccatcc acgaaaaaac cctgtggcgc aaagccggcg ggcacgcctc cctggacagc
     6961 gccatccatg gcgccaacgg agccgccgcc ttgttccgtg gcaaggtgcg catcgactac
     7021 gccacgcacg gtcagccaca ctacgccgtg gtctggcaag aagggcaggg cacgggctac
     7081 gtcatcaagc ccgacgccag ccagccccag gacatccgcg tgcaaagcgt gccggtgcaa
     7141 aagctgcaag ccttcgaggt cgccgggcag gccagcgcgg cgcagctgga agcgctgttt
     7201 cagccttgat gcaaacagat ccctgcttca agcatcaaaa agaggaagac atgcgcacca
     7261 ccctcgacat ctccgacgac gtgctgctgg cggccaaaga gctggcgcgg cgtggcaaaa
     7321 agccgctggg acaggtcatc agcgaactgg cgcgcaaagc cttcacgctg cccgatgacc
     7381 acagcgcggc ggccggtacg gcaaaaaacg cggacaaccc gttggcggcg ctcggcattc
     7441 accccctgcc gccgtgcggc gtgatcgtca gcaacgaaca catcgaccgc ctgcgcgacg
     7501 aagagggcgt ctgacatgcg ggcgctgctc gacgtcaacg tgctgattgc cctgcacgac
     7561 agcaaccaca tccatcacgc cgaagccgcc cgctggctgc aaacgcatgc cgaacaaggc
     7621 tgggccagtt gcccgctgac gcaaaacggt tgcctgcgca tcatgggcca accgggttac
     7681 gccaaccctc agcccttgcc catactcacg gccatgctgg cgcagtccac ggccacgcgt
     7741 tttcaccagt tctggcccga cgacatcagc ctgctcgata gcaggcactt caaccacacc
     7801 cacatccacg gcccgcgcca gctgaccgac atgtacctgc tggcgctggc cttgcaccac
     7861 ggcgggcgct tggtcacatc tgatcatcat ctgaaagggt tctctcgcct gtgcaggtgg
     7921 cgtcgcgccc ggcgcgtcaa cacgggctct caagcaatcg cctcaaaggg cagccaccca
     7981 gttctccacc gccaagccag gcacccgaca aaattcacgc tcgttgttgg tcaccagcac
     8041 ccagccctcg gcgcgtgcat gggcggcaat ccacaggtcg ttgttgccaa tgggctgccc
     8101 ggcgcgctcc agggcggcgc ggatctggcc gtaatgtgca ccggcagtgg gtgccagggg
     8161 cataacctga atggcagctt gcaactgctg cagcaccgcc agcgccttgt cccgggcctg
     8221 gcttttttct gcgccgtgct gcaactcgcc cagggtgacc accgacatgg ccagcgcatc
     8281 agaactgagc tgctcgaacc gcgcgcgcac ggcgggcggg ttgtgcttgg cgatgtagat
     8341 gcagatgttg gtatcgagca ggtagcggat ggccattcaa aacgcctcgc gctcttgggg
     8401 cagggcgtcg ccccggccat cttgcatgaa gtccgccggc aagctggcca gcgcatcaaa
     8461 aatggccgcc gcgctggccg ggcgctcgcg cagcacgatg tcattgccct ggcggaagat
     8521 ctccacctgc tcggcgttga agcgaaattc cttgggcagg cgcacggcct ggctgttgcc
     8581 ggactggaaa acacgagcgt aggtcatgga agtccctcat agatatacgg aaagtatata
     8641 cattgggtgt tgtgtgtcaa acgtggacat ctgcatggcc tgaggtgctg atccaatttc
     8701 accttccagc gaaccgctcc catcccgcca aaattgccgg acaacaggct tgctgagcct
     8761 ggctcttcaa agctcgtcca ttcactgcat ctcaaccctg cccaacaaac tcccaagggt
     8821 tgaacagcgc cacccccatg tgcacaaaat cccgcacatt gcgcgtcacc agcgtcaggc
     8881 catgttgcaa ggccgtggcg gcaatcagcg catcatgcgg tggcgccggg tcgggcacat
     8941 gcagcggcgc gcaacactgc accaccgcgt cgtccacggg caaactgcgc tcggcaaaca
     9001 aggcgcgcac gccatccaac catgcccgca aagcctgtgc ttgcgcggca tcgcgccgtt
     9061 gcaaccgcaa aattccgata tcaatctcca gccacgtcag cacgctcacg tactgggccg
     9121 ccaaaggcac gctggccgcc caggccagca cgcgcgcatc cgcctgcggc ttgccggtgc
     9181 gcaactcgga gatcacgttg gtgtcgagca aatacatcag cgcggctcgt gctcaccaaa
     9241 ttcgggaatg cgcagggtga tgtcagcggc gcccgggcgt tcgggcaaag gcaactcgcc
     9301 ccccgagggc gcatccaccc cccgcatggc ctgcaacaaa ctctcgccgc ccacctgccc
     9361 ggtcaggcgg taatagtcct caatcttgag caccgccaga gagggcttgc cgcgctcggt
     9421 aatgatcacc ggcccctgct gcgccgcacg cttgactgcc gccgcatcgc gcgcaaaggt
     9481 gcggctgttc atggtggcaa tgctcatgac cgcctcctca aattggttgt ctgtatgtta
     9541 cgacaaaaaa acggcgccag caaacagccg tgaaacctgc catgcgaggt cccgcgtacg
     9601 gcaagcttgc gatgcatctc ggagagtcga tgtgggccaa caatccatcc atcgcaacaa
     9661 cacaccagaa ggaaacacac catgactctc ccccaagcca tcgccttcgg caccgccggc
     9721 gacaaactct cgcgcaagat cacgggcacc aacgaggtga gcgtcacccg cagcaccatt
     9781 gccacgggtg ccggcgccgc cctgggcgcg gccggcgcgg gcgcgctatc ggtgggtgcc
     9841 ggcgcggttg gccttaccgc cgtggctacc gtggccgcac cggtggccgt cccagtcgcc
     9901 atcgtcgccg gcggcattgg tttgctgtgc agcctgttcg agtgaccacc tggttcgtca
     9961 gccgccaccc cggcgccctg cactggatgc gccagcacgg cccggcctat gaccagcatg
    10021 tgccgcacct cgaccccgcc caggtgcagc cgggcgatgc cgtcatcggc acgctgcccg
    10081 tgaacctggc ggcgcaggtg tgcgcgcgcg gcgcggcgta ttggcatttg gcgctgggtg
    10141 tgccggcaca aagccgtggg cgtgagctga gtgccgatga actgctggcc ctgggtgcta
    10201 cgctgcaacg ttttaacgtt caatctcaca aattcaaacc tgcaccatga ccacaaccct
    10261 gcacctgtgt attgccaccg gccagaacgc cgccaacttc attccgctca agcaactggc
    10321 ggcgcacacc atctggattc tggaaacgcc cgacatgcaa aagcagcaaa gcgccaaggc
    10381 cttgagcacc gcgctcaagt cagcgctgcc cgaggcgcag atccgcaccc tg
//
LOCUS       DAOMTQ010000107        10391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1261_length_10391_cov_27.675310, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000107 DAOMTQ010000000
VERSION     DAOMTQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_10391_cov_27.675310"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="PK518_10770"
     CDS             <1..1491
                     /locus_tag="PK518_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49441.1"
                     /translation="IFALLAFAAGFIIRPFGALFFGRLGDLIGRKYTFLVTILIMGIS
                     TFIVGILPNYASIGVAAPVILICLRLLQGLALGGEYGGAATYVAEHAPNGKRGAYTAW
                     IQTTATMGLFLSLLVILGTRTLMGEPAFNDWGWRIPFLVSILLLAISVWIRMQLSESP
                     AFRKMKAEGKTSKAPLAESFGQWKNLKIVLIALFGLTAGQAVVWYTGQFYALFFLTQQ
                     LKVDAVTANLLIAAALLIGTPFFLIFGSLSDKIGRKTIIMAGCALAIVSYFPAFKALT
                     QAANPDLAAAQAKSQVFIVADPKECSFQFNPTGTTKFTSSCDIAKQVLAASSVSYDNE
                     EAPAGTPAVIKIGDDVIQSYSAKGLSAADAKAKDAEFKKAVADTLKADGYPAKADPAK
                     MNKVMMVVILTYLVILVTMVYGPIAAMLVELFPTRIRYTSMSLPYHIGNGWFGGLLPT
                     MSFAIVAQTGNMYNGLWYPIIIAGMTLVVGMLFLRETKDVDIYAND"
     gene            1709..2698
                     /locus_tag="PK518_10775"
     CDS             1709..2698
                     /locus_tag="PK518_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRM49442.1"
                     /translation="MQKKHLLLALVAAAGASTVMAQSSVTLYGRVNTSVERQKVGSVS
                     NTVMQNNASRFGFKGVEDLGGGLKAGFQLESGFDSSTGAGSAWTHPTTGMSFARQSEV
                     NLSGGFGMIRLGNFIPDSYYATADYVSMHNHETGTSADAFYQDPVWLTSGLSTKNKVG
                     YRTPNMGGLVIDAAVSLHEKATGVNKNGYDLAANYNLGALALGAGYSQVDDDKQLGLR
                     ALYTFGQFTVGGYYQRNDEDVAGTRNNYRLSGMYALGASEFHVNVGRAAKWSKVADSA
                     ATQWTLGYNYNLSKRTKVYAFYTKVDNKAGAAYMTGTAGADFSSFALGVRHNF"
     gene            2840..3001
                     /locus_tag="PK518_10780"
     CDS             2840..3001
                     /locus_tag="PK518_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49443.1"
                     /translation="MWKLVVGFVLFAALSLFVIMQAGDKVDLSGEKHGADAVHAPEPA
                     APTAAGAAK"
     gene            complement(3068..4180)
                     /locus_tag="PK518_10785"
     CDS             complement(3068..4180)
                     /locus_tag="PK518_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM49444.1"
                     /translation="MRTWQKWGSTLMPGAALGTVQAQILVGQTAGFSGVVGAGVQETA
                     AGAKLYIDAVNAKGGVHGQKIELISLDDKFDPKTAGENARVLIEDKKVDVMFLTRGTP
                     HTEAIIPHLDRHGVALVAPSTGAMVLHQPVRKHVFNVRATYQREAEKAMTHLATLGMN
                     RIAVLYADDSFGADGVAGAQKGLDAARLQPVVLDKFNRAQPDFAPLAARIAQAQAQAV
                     LIIGSGTTVADGYAALRKAGSTAQLVTLSNNASSGFIKSLGEQARGVIVSQVFPNERS
                     LAYPLVKEALELSKAGGQGDISPAMLEGFAAAKVLVEGLRRAGPKPTREKIQAALEAI
                     QQYDLGGLPISYGQKDHTGLDFADLSIIGADGKFRR"
     gene            4302..10376
                     /gene="uvrA"
                     /locus_tag="PK518_10790"
     CDS             4302..10376
                     /gene="uvrA"
                     /locus_tag="PK518_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRM49445.1"
                     /translation="MTQGLIRIHGARQHNLKNLDLDIRTGELTVVTGPSGSGKSSLVF
                     DTLYAEGQRRYVETFSAYARQFLDRMDKPAVDKVEGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLFARAGQLFDRQTAQSVRHDSPDTIYADLQARCAQEGDPRIVLTFPVE
                     LPASITAEQLEQWLSASGFTKVQAEREHDGKKLLDVVADRFRIANTERARALEAIEVA
                     LKRGAGRLNVYRLRDEGEPDLWRFSTGLHCPDSDLRYAEPIASMFSFNSAVGACDSCR
                     GFGRVIGVDWGLVIPNDRLTLRAGAIKPIQTPAWKECQDDLMRYAEDAGIPRDTPWYK
                     LTAEQRHWVIEGTPGFKEGNWNRQWYGIRRFFAYLETKAYKMHIRVLLSKYRSYTPCP
                     SCAGARLKTESLLWRVGGKQDADAVLEPARRFMPQGVDWSREQLEALPGLCLHDLMLL
                     PIERLRRFFAALLPLPQAGEGRGEGGPAAGAALDLPPHPHPLPQGGEGVNTGEIQALA
                     LLHEEIATRLKYLCDVGIGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEP
                     SIGLHPRDMQRINQAMLRLRDAGNTLVVVEHDPAVMLAADRIIDMGPGPGERGGQIVF
                     DGSVAALRRADTLTGQYLGGHRQIGTGLKRMVTEATPRLILEGARQHNLRDVTVEFPL
                     QRLVTVTGVSGSGKSTLIQDILAPALMRHFGKATESPGAHDRLLGADHLSDVVFVDQS
                     PIGKTARSNPVSYVGAWDAIREIFATAPLSRERGYTAAKFSFNSGDGRCPTCGGSGFE
                     HVEMQFLSDVYLRCPDCDGRRYRPEILEVRIERGGRTMSVADVLELTVHEAAQLFAKD
                     REVIRALQPIVDVGLEYVRLGQPVPTLSGGEAQRLKLAGFLAEAARSQAKSRQPLARK
                     GTLFLFDEPTTGLHFDDIAKLMRALRKLMEAGHSLIVIEHNLDVIRASDWLIDLGPEG
                     GDAGGQLVAEGTPEDLRQHPSSHTGAALREYEQALGEGGHAVHDGVPPYDSLSRLRER
                     AGVRGDAQGAGPHPHPLPEGEGAAANAIQIINAREHNLKHLNVDIPRGRFNVITGVSG
                     SGKSTLAFDILFNEGQRRYLESLNAYARSIVQPAGRPEVDAVYGIPPTVAIEQRLSRG
                     GRKSTVGTTTEVWHFLRLLYVKLGVQHCVHDGAAVQPQTSESIAAQLLTQFRDQHIGL
                     LAPLVLNRKGVYTELADWARPRGYTHLRVDGNFLPTTGFPRLDRFKEHTIELPVASLD
                     VSPEHEDALRSALSKALELGKGVLHVLSGLDGLEQAMEEGAPTAHIGRLQVFSTKRAC
                     PVCATSYAELDPRLFSYNSKHGWCPDCVGTGVRLTKEQRKAFDDSVQSDDRGREQTFA
                     EPEVEDLADAACPSCAGTRLNPVARAVHFAGTPITEVARLAVSEVRQWIEGLQLTGRE
                     AEIARDLVPEIKSRLEFLEEVGLSYLTLDRGAPTLSGGEAQRIRLAAQLGSNLQGVCY
                     VLDEPTIGLHARDNQILLNALKSLSDKGNTLVVVEHDEDTIRRADHLIDIGPSAGKRG
                     GCLVAQGQVADLQAAADSQTGRYLLHAMRHPLQARRAVFLPPLPGEGRGGGQSLLDEK
                     YEQNKPQTLDGKAQIAINLDVADPYPHPPPEGEGVNTAAAATPSPPAGGEGRGEGVSA
                     PAPHWLTVLGAHLHNLQAVDLRLPLKRLVAVTGVSGSGKSTLARDVLLANVAAWVGQR
                     ATKAGREAMDAGKAPALVGCAGLQGFESVDRVLEVDQTPIGKTPRSCPATYIGFWDTI
                     RKLFAETLEAKARGYAPGRFSFNTGEGRCPGCEGQGLRTIAMSFLPDVKVACERCHGA
                     RFNPETLAVTWRGKNIGDVLQMEVDEAVEFFASMPSIAHPLQLLKDVGLGYLTLGQPS
                     PTLSGGEAQRIKLVTELTKVRDEVGRRGQKAPHTLYVLDEPTVGLHMADVHKLIQVLH
                     RLVDGGHSVVVIEHDLDVIAEADWVIDLGPEGGKDGGRIVAAGTPEELVRLATHTGVA
                     LAPVLARG"
BASE COUNT         1783 a         3527 c         3391 g         1690 t
ORIGIN      
        1 atctttgccc tgctggcctt tgccgcgggc ttcatcatcc ggcccttcgg cgcgctgttc
       61 ttcggccgcc tgggcgacct gatcgggcgc aagtacacct ttctggtgac catcctgatc
      121 atgggcatct cgaccttcat cgtcggcata ctgcccaact acgccagcat aggcgtggcc
      181 gcgccggtga tcctgatctg cctgcgcctg ctgcaaggcc tggcgctggg cggtgaatac
      241 ggcggcgccg ccacctatgt ggccgagcat gcccccaatg gcaagcgcgg cgcctacacg
      301 gcgtggatcc agaccaccgc caccatgggc ctgttcctgt cgctgctggt catcctgggc
      361 acgcgcacgc tgatgggcga gccggccttc aacgactggg gctggcgcat tcctttcctg
      421 gtgtccatcc tgttgctggc catctcggtg tggatccgca tgcagctgtc cgagtcgccc
      481 gccttccgca agatgaaggc cgagggcaag acctccaagg cgcccctggc cgagtccttc
      541 ggtcagtgga agaacctgaa gatcgtgctc atcgcgctgt tcggcctgac cgccggccag
      601 gccgtggtct ggtacacggg ccagttctat gcgctgttct tcctgacgca gcagctcaag
      661 gtggacgccg tcacggcgaa cctgctgatc gccgccgcgc tgctgatcgg cacgcccttc
      721 ttcctgatct ttggctcgct gtccgacaag attggccgca agaccatcat catggcaggc
      781 tgcgccctgg ccatcgtgtc ctacttcccg gccttcaagg ccctgaccca ggccgccaac
      841 ccggatctgg cagcggccca ggccaagagc caggtgttca tcgtcgccga ccccaaggaa
      901 tgctcgttcc agttcaaccc cacgggcacg accaagttca ccagctcctg cgacatcgcc
      961 aagcaggtgc tggccgccag ctcggtcagc tatgacaacg aggaagcccc cgccggcacg
     1021 cccgccgtca tcaagattgg cgacgatgtg atccagagct actccgccaa gggcctgtcc
     1081 gcggccgacg ccaaggccaa ggacgccgag ttcaagaagg ccgtggccga cacgctcaag
     1141 gctgacggct accccgccaa ggccgacccg gcgaagatga acaaggtgat gatggtcgtc
     1201 atcctgacct acctggtgat cctggtgacc atggtctacg gccccatcgc cgccatgctg
     1261 gtggagctgt tccccacgcg tatccgctac acctcgatga gcctgcccta ccacatcggc
     1321 aatggctggt tcggcggcct cctgcccacc atgtcgttcg ccatcgtggc ccagaccggc
     1381 aacatgtaca acggcctgtg gtacccgatc atcattgccg gcatgacgct cgttgtcggc
     1441 atgctgttcc tgcgcgagac caaggacgtg gacatctacg ccaacgactg agcgttcttg
     1501 cctcggggtc ggcgcgacga ccccttcatg acagccccgc cccggcgggg ctgttgcgtt
     1561 taaacgtcag attgcaaacc gattacaggg cctacgggtg cctgctccta aaatcgaggc
     1621 tgtagatgta caaggcgctc atcggtcgaa tgcggatcgc aagcggtgcc tactacccct
     1681 gatagacaca aacattcaag gaacccatat gcaaaagaaa cacctcctgc tcgccctggt
     1741 tgcagcagcc ggcgcctcca ccgtcatggc gcaaagcagc gtcaccctgt atggccgcgt
     1801 gaatacctct gttgagcgcc aaaaggttgg gtcggtcagc aataccgtga tgcagaacaa
     1861 cgcttcgcgc tttggcttca agggcgtgga agacctgggc ggcggcctga aggccggctt
     1921 ccagctggaa agcggtttcg attccagcac cggcgcaggc tccgcctgga cgcaccccac
     1981 taccggcatg agcttcgcgc gtcagagtga agtgaacctg tccggtggct tcggcatgat
     2041 ccgtctgggc aacttcatac ccgactccta ctatgcgacg gccgactacg tgagcatgca
     2101 caaccatgag acgggcacct cggcggacgc gttttatcag gatcccgtct ggctcactag
     2161 cggcctgagc accaagaaca aggttggcta ccgtaccccc aacatgggtg gtctggtgat
     2221 cgacgctgcc gtgagcctgc atgaaaaggc caccggtgtg aacaagaatg gctacgacct
     2281 ggcagccaat tacaacctgg gcgccctggc tctgggtgct ggctacagcc aggttgatga
     2341 tgacaagcaa ctgggcctgc gcgctctgta caccttcggc cagttcacgg tcggtggtta
     2401 ctatcagcgc aacgacgaag acgtggcagg cacgcgcaac aactaccgcc tgtccggcat
     2461 gtacgccctg ggcgcctccg agttccacgt caacgtgggc cgtgccgcca agtggagcaa
     2521 ggtcgccgac agcgccgcca cgcagtggac cctgggctac aactacaacc tgagcaagcg
     2581 caccaaggtc tacgccttct acaccaaggt ggacaacaag gctggtgctg cttacatgac
     2641 cggtactgcc ggcgcggact tcagctcctt cgccctgggc gtgcgccaca acttctgatc
     2701 tcccgcagcg taggctgcct gcaacccagg cctgattcaa tcgagtcggg cttggagcca
     2761 gagagaacag gcctcacaca tccgtgtggg gccttttttg tgggcggccc gttctcacaa
     2821 caaccatcag gagacaggca tgtggaagct ggtagtggga ttcgtgctgt tcgcggcgct
     2881 atcgctgttc gtgatcatgc aggccgggga caaggtggac ctgagcgggg aaaagcatgg
     2941 agcggacgcc gtgcatgcgc ccgagccggc cgcgccaacg gccgctggcg ccgccaagta
     3001 gtgccaccgc ttggcaacaa aaaaacccgc gcaggcgggt tttttttacg ggctgtgcgg
     3061 ggcgccttca gcggcggaac ttgccgtctg cgccaatgat ggacagatcg gcgaagtcga
     3121 gacctgtgtg atccttctgg ccgtatgaaa tcggcaggcc gcccaggtcg tactgctgta
     3181 tggcctccag cgccgcctgt atcttctcgc gcgtgggctt ggggccggcg cggcgcaggc
     3241 cttccaccag caccttggcg gcggcaaagc cctccagcat ggcggggctg atgtcgccct
     3301 ggccgccggc cttgctcagt tccagggctt ccttgaccag cggataggcc aggctgcgct
     3361 cgttcgggaa cacctggctg acgatgacgc cgcgcgcctg ctcgcccagg ctcttgatga
     3421 aaccgctgga ggcgttgttc gacagggtga ccagctgcgc cgtggaaccc gccttgcgca
     3481 gcgccgcata gccgtctgcc accgtggtgc ccgagccaat gatcagcacc gcctgggctt
     3541 gcgcctgggc gatcctggcc gccagcggag caaagtcggg ttgggcacgg ttgaacttgt
     3601 ccagcaccac cggctgcagc ctggctgcgt ccaggccttt ctgggcgccg gccacgccgt
     3661 cggcgccaaa gctgtcgtcg gcatacagca cggcgatgcg gttcatgccc agcgtggcca
     3721 gatgggtcat ggccttctcg gcctcgcgct ggtaggtggc gcgcacgttg aacacatgct
     3781 tgcgcaccgg ctggtgcagc accatggcgc cggtggaggg cgccaccagg gccacgccat
     3841 gcctgtccag gtgcgggatg atggcctcgg tatgcggcgt gccgcgggtg aggaacatca
     3901 catcgacctt tttgtcctcg atcagcacgc gggcgttctc gccggcggtc ttggggtcga
     3961 acttgtcgtc cagcgagatc agctcgatct tctgcccatg cacgccgccc ttggcgttca
     4021 cggcgtcgat atagagcttg gcgccggcgg cggtttcctg cacgcccgcg cccaccacgc
     4081 ccgagaaccc cgccgtctgg cccaccagaa tctgcgcctg tacggtgccg agggccgccc
     4141 ccggcatcag ggtgctaccc catttttgcc atgtgcgcat ggtatcgcct ccttgctttt
     4201 gtcaacaaat gttttgcggt gcggtgcagt ctaacggaca cgacgctcaa tgggcaatcc
     4261 gaatccctag aatgttccgc ctcaccgaac aaagaccggc catgacccag ggactgatac
     4321 gcatccacgg cgcgcgccag cacaacctca agaacctcga cctggacata cgcacgggtg
     4381 aactgaccgt ggtcaccggc cccagcggct cgggcaagtc cagcctggtg ttcgacaccc
     4441 tgtacgccga gggccagcgt cgctacgtgg agaccttcag cgcctatgcg cgccagttcc
     4501 tcgaccgcat ggacaagccg gccgtggaca aggtggaggg cgtgccgccg gcgattgcca
     4561 tcgaccagac aaacccggtg cgcagctcgc gctccaccgt gggcacgatg accgagctga
     4621 acgaccacct gaaactgctg ttcgcgcgcg ccggccagct gttcgaccgc cagacggcgc
     4681 agagcgtgcg ccacgactcg cccgacacga tttacgccga cctgcaggcg cgctgcgcgc
     4741 aagagggcga cccgcgcatc gtgctcactt tcccggtgga gctgcccgcc agcatcacag
     4801 ccgagcagct ggagcaatgg ctgtcggcca gcggcttcac caaggtgcag gccgagcgcg
     4861 agcacgatgg caagaagctg ctggacgtgg tggccgaccg ttttcgcatc gccaacaccg
     4921 agcgcgcgcg cgcgcttgag gccatcgagg tggcgctcaa gcgtggcgcc gggcggctca
     4981 acgtgtatcg cctgcgcgat gagggcgagc cggacttgtg gaggttctcg accggcctgc
     5041 actgccccga cagcgacctg cgctacgccg agcccatcgc ctcgatgttc tcgttcaact
     5101 cggccgtggg cgcctgtgac agctgccgcg gctttggccg cgtcatcggt gtggactggg
     5161 gcctggtgat tccgaacgac aggctgacgc tgcgcgccgg cgccatcaag ccgattcaga
     5221 cgccggcctg gaaggaatgc caggacgacc tgatgcgcta tgccgaggac gccggcattc
     5281 cgcgcgacac gccctggtac aagctcacgg ccgagcagcg gcattgggtg atagagggca
     5341 cgccgggctt caaggagggc aactggaacc gccagtggta tggcatcagg cgcttcttcg
     5401 cctacctgga gaccaaggcc tacaagatgc acatccgcgt gctgctgtcc aagtaccgca
     5461 gctacacgcc atgccccagc tgcgccggcg cgcgcctgaa gaccgagagc ctgctatggc
     5521 gcgtgggcgg caagcaggat gccgatgcgg tgctggagcc cgcgcggcgc ttcatgccgc
     5581 agggcgtgga ttggagccgt gagcagctgg aggcgctacc ggggctgtgt ctgcatgatt
     5641 tgatgctgct gccgattgag cggctgcggc ggttttttgc ggcgcttctc cctctcccgc
     5701 aggcgggaga gggcaggggt gagggtgggc ccgcagctgg cgctgcgctc gatctacccc
     5761 ctcaccccca ccctctcccc caagggggcg agggagtaaa taccggggag atacaggccc
     5821 tggcgctgct gcacgaagag attgccaccc gcctcaaata cctgtgcgac gtcggcatcg
     5881 gctatctcac gctggatcgg caaagccgca cgctgtcggg cggcgaggtg cagcgcatta
     5941 acctgaccac ggccctgggc acgtccctgg tcaacaccct gttcgtgctc gacgagccca
     6001 gcataggcct gcacccgcgc gacatgcagc gcatcaacca ggccatgctg cgcctgcgcg
     6061 atgccggcaa caccctggtg gtggtggagc atgacccggc ggtgatgctg gcggccgacc
     6121 gcatcatcga catgggcccc ggcccgggcg aacgcggcgg gcagatcgtg ttcgacggca
     6181 gcgtggccgc gctgcgccgg gcggatacgc tgaccggcca atacctgggc gggcacaggc
     6241 agataggcac gggcctcaag cgcatggtca ccgaggccac gccgcgcctg attctcgaag
     6301 gcgcacgcca gcacaacctg cgggacgtga cggtggagtt cccgctgcag cgcctggtca
     6361 ccgtcaccgg cgtgtcgggc tcgggcaagt ccacgctgat ccaggacatc ctggcgcccg
     6421 cgctgatgcg ccacttcggc aaggccacgg agtcacccgg cgcccatgac cggctgctgg
     6481 gcgcggacca tttgagcgac gtggtgttcg tggatcagtc ccccattggc aagacggcgc
     6541 gttcgaaccc cgtgagctat gtaggcgcgt gggacgccat tcgcgagatc ttcgccaccg
     6601 cgccgctgtc gcgcgagcgc ggctacacgg ccgccaagtt cagcttcaac tcgggcgacg
     6661 ggcgctgccc cacctgcggc ggctcgggct tcgagcatgt ggagatgcag ttcctgtcgg
     6721 acgtctatct gcgctgcccg gactgcgacg gccggcgcta ccggcccgag atcctggagg
     6781 tgcgcataga gcgcggaggg cgaacgatga gtgtggccga tgtgctggag ctgaccgtgc
     6841 acgaggccgc ccagctgttc gccaaggatc gcgaggtgat ccgcgccctg cagcccatag
     6901 tggacgtggg cctggagtac gtgcgcctgg gccagcccgt gcccacgcta tccgggggcg
     6961 aggcccagcg cctgaagctc gccggctttc tggccgaggc cgccaggagc caggccaagt
     7021 cgcgccagcc gctggcgcgc aagggcacgc tgtttttgtt cgacgagccc accaccggcc
     7081 tgcatttcga cgacatcgcc aagctgatgc gcgcgctgcg caagctcatg gaagcgggcc
     7141 attcgctgat tgtcatcgag cacaacctgg acgtgatccg cgccagcgac tggctcatcg
     7201 acctgggccc cgagggcggc gacgccggtg gccagctggt ggccgagggc acgcccgaag
     7261 acctgcgcca gcaccccagc tcgcacaccg gcgcggcgct gcgcgagtat gagcaggcgc
     7321 tgggcgaggg tggccatgca gtccacgacg gcgtgccgcc ctatgactcc ctctcccgct
     7381 tgcgggagag ggcaggggtg aggggggatg cccagggcgc cggcccccac ccccaccctc
     7441 tcccagaggg agagggtgca gcagccaacg ccattcagat catcaacgcc cgcgagcaca
     7501 acctcaagca cctgaacgtc gacatcccgc gcggcaggtt caacgtcatc accggcgtca
     7561 gcggctcggg caagtccacg ctggccttcg acatcctgtt caacgagggc cagcgccgct
     7621 atcttgaaag cctgaacgcc tacgcgcgca gcatcgtcca gcccgcgggc cggcccgagg
     7681 tggacgccgt gtatggcata ccgcccacgg tggcgataga gcagcgcctg tcgcgcggcg
     7741 gcaggaagag caccgtgggt acgaccaccg aggtctggca ctttctgcgc ctcttgtacg
     7801 tcaagctcgg cgtgcagcac tgcgtgcacg acggcgcggc cgtgcagccg cagaccagcg
     7861 agagcatcgc cgcgcagctg ctcacgcagt tccgcgacca gcacatcggc ctgctggcgc
     7921 ccctggtgct gaaccgcaag ggcgtctata ccgagctggc cgactgggcg cgcccgcgcg
     7981 gttacacgca tctgcgcgtg gacggcaact ttctgccgac taccggcttc ccgcgcctgg
     8041 accgcttcaa ggaacacacc atagagctgc ccgtggccag cctggatgtg tcgcccgagc
     8101 acgaagacgc cctgcgcagc gcgttgtcca aggcactgga gctgggcaag ggcgtgttgc
     8161 atgtgctgag cggcctggac ggcctggagc aggccatgga agagggcgcg cccacggcgc
     8221 atatcggccg gctgcaggtg ttctccacca agcgcgcctg cccggtctgc gccaccagct
     8281 atgccgagct ggacccgcgc ctgttctcgt acaacagcaa gcatggctgg tgcccggact
     8341 gcgtgggcac gggcgtgcgc ctgacgaaag agcagcgcaa ggccttcgac gattccgtgc
     8401 agtcggacga ccgcggccgc gaacagacct ttgccgagcc cgaggtggaa gacctggccg
     8461 acgccgcctg ccccagctgc gccggcacgc gcctgaaccc cgtggcgcgc gccgtgcact
     8521 ttgccggcac gcccatcacc gaggtggcgc gcctggccgt gagcgaggta cggcagtgga
     8581 tagagggcct gcaactcacc ggccgcgagg ccgagatcgc ccgtgacctg gtgcccgaga
     8641 tcaaaagccg gctggagttt ctggaggagg tgggcctgtc ctacctgacg ctggaccgcg
     8701 gcgcgcccac gctcagcggt ggcgaggccc agcgcatccg cctggccgcc cagctgggca
     8761 gcaacctgca gggcgtgtgc tacgtgctgg acgagccgac cataggcctg cacgcgcgcg
     8821 acaaccagat cctgctgaac gccctgaaaa gcctgagcga caagggcaac accctggtcg
     8881 tcgtggagca tgacgaggac accatacgcc gcgccgacca cctcatcgac ataggcccca
     8941 gcgccggcaa gcgcggcggg tgcctggtgg cccagggcca ggtggccgat ctgcaggccg
     9001 ccgccgactc gcagaccggc cgctacctgc tgcatgccat gaggcatccg ctgcaggcgc
     9061 ggcgtgcggt atttctccct cccctcccgg gggagggcag gggtgggggc cagtctctct
     9121 tagatgaaaa atatgagcaa aataagcctc aaacgcttga tggtaaagcg caaatagcta
     9181 tcaatttaga tgttgctgac ccttaccccc accctccccc agagggggag ggagtgaata
     9241 ccgccgcggc tgctactccc tctccccccg cagggggaga gggcaggggt gagggggtat
     9301 ccgccccggc accgcactgg ctcaccgtcc tcggcgccca cctgcacaac ctgcaggccg
     9361 tggatctgcg cctgccactg aaacgcctgg tggccgtcac gggggtcagc ggctcgggca
     9421 agtccacgct ggcgcgcgac gtgctgctgg ccaatgtggc tgcctgggtc ggccagcgcg
     9481 ccaccaaggc cgggcgcgag gccatggatg ccggcaaggc gcccgcgctc gtgggctgcg
     9541 ccggcctgca gggcttcgag agcgtggatc gcgtgctcga ggtcgatcaa acccccatag
     9601 gcaagacccc gcgcagctgc cccgccacct acatcggctt ctgggacacc atacgcaagc
     9661 tgttcgccga aaccctggag gccaaggcgc gcggctacgc gcccgggcgc ttcagtttca
     9721 acaccggcga gggccgctgc cccggctgcg agggccaggg cctgcgcacc atcgccatga
     9781 gctttctgcc cgacgtcaag gtggcctgcg agcgctgcca cggcgcgcgc ttcaaccccg
     9841 agaccctggc cgtcacctgg cgcggcaaga acattggcga tgtgctgcag atggaggtgg
     9901 acgaggccgt cgagttcttc gccagcatgc ccagcattgc ccacccgctg cagctcttga
     9961 aggacgtggg cctgggctac ctcacgctgg gccaaccctc gcccacgctc tccggcggcg
    10021 aggcccagcg catcaagctg gtgacggagc tgaccaaggt gcgcgacgag gtcggccggc
    10081 gcgggcaaaa ggcaccacac acgctgtatg tgctggacga gcccaccgtg ggcctgcaca
    10141 tggccgacgt gcacaagctg atccaggtgc tgcaccgcct ggtcgacggc ggccacagcg
    10201 tggtcgtcat cgagcacgac ctggacgtga tcgccgaggc cgactgggtc atcgacctgg
    10261 ggcccgaggg cggcaaggac ggcgggcgca tcgtcgccgc cggtacgccc gaggagctgg
    10321 tgcgactggc cacgcatacc ggggtggcgc tggcgccagt gctggcgcgc ggttgaggta
    10381 ttggctccct c
//
LOCUS       DAOMTQ010000108        10367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1265_length_10367_cov_29.098914, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000108 DAOMTQ010000000
VERSION     DAOMTQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1265_length_10367_cov_29.098914"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1081
                     /locus_tag="PK518_10795"
     CDS             164..1081
                     /locus_tag="PK518_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM49446.1"
                     /translation="MAQKMVQDKVVVVTGAGGGIGRDMALALAAAGAKVVVNDIGTST
                     SGEGQDAGPAQKVVEEIKAAGGQAVANTDSVAEAVAAGRIVQCALDSFGRIDGVVNNA
                     GILRDRFFHKMSLDEWDAVLKVHLYGSYYMSRAAANHFKEQESGTFVHMTSTSGLIGN
                     FGQANYSAAKLALVALSKSIALDMAKFNVRSNCIAPFAWSRMIGSIPTDTPEQQARVA
                     KIQQMTPNKIAPLAVYLLSDAAKDVNAQVFAVRNNEIFLMSQPRPVRSVHRSEGWTAE
                     SIAEHGMPALTRSFVPMERSGDVFSWDPV"
     gene            1092..1982
                     /locus_tag="PK518_10800"
     CDS             1092..1982
                     /locus_tag="PK518_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRM49447.1"
                     /translation="MAIDYHHLKNRPFAPVHQHYQARDSMLYALSLGLGNDPLDAAAL
                     PFVYEGMEGGLRALPTLAVVLGYPGFWARESDTGIDWVKLLHGEQRVRWHQPLPADCE
                     VVGKSRVTHLTDKGEGKGAIMITERRVETAAGALLATMQQVTFLRGDGGYSQRAGQAS
                     DEPLPPLKHTPEDRAPDFTDTQVIRPEAALLYRLMGDYNPLHAEPAVAAKAGFERPIL
                     HGLASYGLVAWALLRQCAGFEPARLKALDIRFAAPVYPGETLITEIWRVPGDGKQYQL
                     RARVAERDKVVLSHGFAELA"
     gene            2112..2909
                     /locus_tag="PK518_10805"
     CDS             2112..2909
                     /locus_tag="PK518_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRM49448.1"
                     /translation="MSTPPVLTAIADGIGTITLNRPEARNALSKDMRPALAAAVTQMR
                     DDPQVYAVILTGAGGAFCSGGDISGMLDSTRTGLVFRNNMRELHQWLPELVNMEKPVI
                     AAVDGPAFGAGLSLALAADFVLATRRAKFCAVFGRIGLVPDLGMLHQLPRIVGLQKAK
                     ELVFTARTVEAEEAKQLGMVFDIVDDGAALTVAAQALAKRFGDASTAAIGMAKTIMNQ
                     SFESDARTLAEMEAYAQSMCRGSPYHQEAIRRFKAKEPLRFDWDRAP"
     gene            2906..4183
                     /locus_tag="PK518_10810"
     CDS             2906..4183
                     /locus_tag="PK518_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRM49449.1"
                     /translation="MSADLARHLQQLIRPGDTLWWGQATAEPLTLTRALVEHRHAIAQ
                     GGRLGVFVGMAASDTLRPEQADVLDFFGYAAGGAHRQLADAGRLDILPSHYSHLPGLI
                     QGGVLPADVLLLQVSKPDAAGRHSLGLVREYLPAVLAKARVVIAEVNPSIPWTHGGPY
                     LQAGDFALLVDAEHLPLDQPRGAIGATEQQIARHIAGLIEDGATLQLGVGNLPEAVLA
                     ALHGHRDLGLHTGAVTDGIAQLAQAGVLTNAQKSIDRGVGIGGILMGSDILRRWAECN
                     PQLELRATDYTHDPVVLAKSHKLAAINAAIEVDLTGQINAEVAAGCYVGAVGGAVDFL
                     RGAARSYGGLPIVALPATAKGASRIVAQLSGPVSTPRSDAGLIVTEHGVADLRGQPLS
                     RRVRRLIAIAAPEHRESLERQAHELLRRCGAVL"
     gene            4256..5464
                     /locus_tag="PK518_10815"
     CDS             4256..5464
                     /locus_tag="PK518_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRM49450.1"
                     /translation="MRRAAIVTPLRTPVGTFGGSLRPVSVEELAATTVRAVVQKSGID
                     PTRIDDVVFAQSYASSETPCVGRWAALQAGLPVSVPGMQLDRRCGGGLQAIVTAAMMV
                     QSGAADVVIAGGVESMSNIEYYSTDMRWGARSGNVRFYDRLDRGRERSQPVERFGKIS
                     GMIETAENLARDYQITREQADAFALRSHQKAAAAWAAGRFADEIVPVQVPQRKGDPIL
                     FSQDEGFRADATPESLGKLRALMPNGTVTAGNASQQNDASAACLIVAEDKLAELGLTP
                     MATLVGWAAAGCEPSHMGIGPVPAVQKLLARLNLKIDDMDLVELNEAFACQVLAVLKG
                     WGWQDQDAIAHKLNVNGSGISLGHPIGATGVRILATLLHELQRRKGRYGLETMCIGGG
                     QGIAAVFERV"
     gene            5570..6547
                     /locus_tag="PK518_10820"
     CDS             5570..6547
                     /locus_tag="PK518_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49451.1"
                     /translation="MPSCRRLAIAALALAPLALASTPAAAQDGQPVRLIVGYAAGGPV
                     DQAARLFAPLLGKELGQSVIVENKTGANATIAGSEVVRAKPDGLTLWYAASPTITISP
                     NVMSKMPFDPAKDLTPVAPILSYYNVLVVNNNEPYKSVRELVATAKSNPGKLAYGSAG
                     VGGSNHLGALLFAKRSGIEMNHIPYKGNAPAMTDVIGGQLNMMLDIISTASAYIHSGK
                     VRAIAVTSPQRNASLPDVPTFAESGIDGLKGFDVGGWYGVYGPKGMSPELVAKLNKAV
                     NAALQQPELKKRYKELGYDEWTGDAHKLAERAAKERAMWATVTQGITVD"
     gene            6549..8057
                     /locus_tag="PK518_10825"
     CDS             6549..8057
                     /locus_tag="PK518_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRM49452.1"
                     /translation="MAARIHQLLDRWLAQAPDRPFIHLPDGRSLSFADLGALTDAAEA
                     ELRQLGVRAGDRVLVVAENCPEHAALILAASRVGAWSCGVNARMAPGEVDAFAAKADA
                     RLLYFTASGSRAAAGHARRHAGVASVLPGMQHGAVREQATVEPPPLQDDVAALIFTSG
                     TTGDPKGVMVTHEGLIHFARISAQSRALGPRDRSYAFVPMTHIFGLATVLLASLWAGA
                     QLVLRPQFDPAELLDALAHHGVTQLQGPPALFSRLLGYLHEQGMEHPEAPALRYVYTG
                     AGPLDLTLKHRVEATFGQALHHGYGLSEYAGSVHLTRMGEQRSDTSVGYVVEDAEVQV
                     TDPATGQALPTGERGELWIRGRGLMRGYFRDPAATAAVMKAGGWYASGDLGELHADGA
                     LFVVGRLKEMIIRSGFNVYPAEVELALNQLPGVQRSAVVGRPEKDGNEEVIAFVELSP
                     GASFDETAARARLREQLAPYKLPSHIVPVAELPTSTNGKVLKRQLQEQAATL"
     gene            8164..9150
                     /locus_tag="PK518_10830"
     CDS             8164..9150
                     /locus_tag="PK518_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49453.1"
                     /translation="MTTHRITRRQLVAAGLLAAATQPFAALANDAWPAKPVRFIVPFP
                     PGGPVDTTARAASLKLSEIWKTPTVIDNRAGAGGVVGAQAAAREAPDGYTLFMGAIHH
                     AVNPTLMGNLPYDIEKDFAPVSFGAMFPVFVVVHPSVPANTLQELIALAKKPGSNLSY
                     ASSGNGGGTHLAGELFNMEAGTKLQHVPYKGSAPAMNDLLGGQVQIMFSDAPTALPHI
                     KSGRVKVLAVASRKRSAMLPDVPAAAEAGLPNYEAYSWTALFAPAKTPQAVLDKINAD
                     FNTAFKDPAVRQRLLDGGAETAPGTQQEMRDFLRAEIAKWAKVIKTAGITTG"
     gene            complement(9233..>10367)
                     /locus_tag="PK518_10835"
     CDS             complement(9233..>10367)
                     /locus_tag="PK518_10835"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRM49454.1"
                     /translation="ARLELLASADTFDWHAEEGRRNYGRVVPGRHLHQRITVLHEPVG
                     VCAAFTPWNFPAITPARKMAGALAAGCTLIIKLAEETPATGLALVRALHDAGLPAGVL
                     NAVFGVPAEVSTYLLDSPIVAKFSFTGSTAVGKQLAQQAARSLKRATMELGGHAPAIV
                     CADAEVERAADMLIAGKFRNAGQVCIAPTRIYVLEPVYEAFVHAFLERARQLTPGNGL
                     LDGSSMGPLANPRRVQAMRSLIADAVERGAQLQCGGDAMAGPGYFWQPTVLTDVPEDA
                     RVMNEEPFGPLALINRAHSVDEALERANRLPYGLAAYAFTHDSATRMHLARGLQTGML
                     GINSLHVSMPEAPFGGVKESGWGSECGIEGLQEYCSIKLISED"
BASE COUNT         1704 a         3626 c         3524 g         1513 t
ORIGIN      
        1 cgcgcaatcc gcgtttgtga aagcggccct tccgcgctca tgaaggccag ggcctgcttt
       61 catgcagcca aagcccgacc tagccattgc cgaatggcgc cggcgcggct ttgccggcat
      121 gcttggcgcc cattgatcta cttacaacgc aaggatgacg gacatggcgc aaaagatggt
      181 gcaggacaag gtggtggtgg tcacgggcgc tggcggcggc attgggcgcg acatggcgct
      241 ggcgctggcc gcggcggggg ccaaggtggt ggtcaacgac attggcacct ccaccagcgg
      301 cgagggccag gacgccggcc cggcgcagaa ggtggtggaa gaaatcaagg ccgccggtgg
      361 ccaggccgtg gccaataccg acagcgtggc cgaggccgtg gccgccgggc gcatcgtgca
      421 atgcgcgctc gacagctttg gccgcatcga cggcgtggtg aacaacgccg gcatcctgcg
      481 cgaccgcttc ttccacaaga tgagcctgga cgagtgggac gcggtgctca aggtgcacct
      541 gtacggcagc tactacatga gccgcgctgc ggccaaccat ttcaaggagc aggagagcgg
      601 caccttcgtg cacatgacct ccacctcggg cctgatcggc aacttcggcc aggccaacta
      661 cagcgcggcc aagctggcac tggtggcgct gtccaagagc attgcgctgg acatggccaa
      721 gttcaacgtg cgctccaact gcatcgcgcc ctttgcctgg agccgcatga tcggctccat
      781 ccccaccgac acgcccgagc agcaggcgcg cgtggccaag atccagcaga tgacgcccaa
      841 caagatcgcg ccgctggcgg tctacctgct gtcggatgcg gccaaggacg tgaacgccca
      901 ggtgttcgcc gtgcgcaaca acgagatctt tttgatgagc cagccgcgcc ccgtgcgctc
      961 ggtgcaccgc agcgagggct ggacggcgga gagcatcgcc gagcatggca tgccggctct
     1021 gacccgatcc ttcgtgccga tggagcgttc cggcgacgtg ttctcctggg atccagtctg
     1081 agggcgcgcg catggccatc gactaccacc atctgaaaaa ccgcccgttc gcccccgtgc
     1141 accagcacta ccaggcgcgc gacagcatgc tgtacgcgct cagcctgggg ctgggcaacg
     1201 acccgctcga tgccgccgcc ctgcccttcg tctacgaggg catggaaggc ggcctgcgcg
     1261 ccctgcccac gctggccgtg gtgctgggct accccggctt ctgggcgcgc gagagcgaca
     1321 cgggcattga ctgggtcaag ctcttgcacg gcgagcagcg cgtgcgctgg caccagccgc
     1381 tgccggccga ctgcgaggtg gtcggcaaga gccgcgtcac ccacctgacc gacaagggcg
     1441 agggcaaggg cgccatcatg atcaccgagc gccgtgtgga aaccgccgca ggcgcgctgc
     1501 tggccaccat gcagcaggtg acatttctgc gcggcgacgg cggctatagc cagcgcgcgg
     1561 ggcaggcgag cgacgagccg ctgccaccgc tcaagcacac gcccgaggat cgcgcgcccg
     1621 acttcaccga cacgcaggtc atacgccccg aggcggcgct cttgtaccgc ctgatgggcg
     1681 actacaaccc gctgcacgcc gagccggcgg tggccgccaa ggccggcttc gagcgcccca
     1741 tattgcacgg cctggccagc tacggcctgg tggcctgggc gctgctgcgc cagtgcgcgg
     1801 gctttgaacc ggcgcgcctt aaggccctgg acatccgctt tgccgcgccg gtctatccgg
     1861 gcgagaccct catcaccgaa atctggcgcg tgccgggcga tggcaagcag taccagctgc
     1921 gcgcccgcgt cgcggaacgc gacaaggtgg tgttgagcca cggcttcgcc gagctggctt
     1981 gaattcactc cctctcccgc gagcgggaga gggcaggggt gagggcgaca aaaatgggcg
     2041 ctggactcaa caccccctca cccccgccct ctccccctcg gggggagagg gagtaacaac
     2101 caggagactc gatgagcaca ccccccgtat tgaccgccat agccgacggc ataggcacca
     2161 tcaccctgaa ccgccccgag gcgcgcaacg cgctcagcaa ggacatgcgc ccggccctgg
     2221 ccgcggccgt cacgcagatg cgcgacgacc cgcaggtcta cgccgtgatc ctcaccggcg
     2281 cgggcggcgc cttctgctcg ggcggcgaca tctccggcat gctcgacagc acgcgcaccg
     2341 gcctggtctt tcggaacaat atgcgcgagc tgcaccagtg gctgcccgag ctggtgaaca
     2401 tggaaaagcc ggtcatcgcc gccgtcgatg gcccggcctt cggcgccggc ctgagcctgg
     2461 cgctggcggc cgacttcgtg ctggccacgc gccgcgccaa gttttgtgcg gtgttcggcc
     2521 gcatcggcct ggtaccggat ctgggcatgc tgcaccagct gccgcgcatc gtgggcctgc
     2581 aaaaggccaa ggagctggtc ttcaccgcgc gcacggtgga ggcagaggag gcaaaacagc
     2641 tcggcatggt gttcgacatc gtggatgatg gcgccgccct caccgtggcc gcgcaggcgc
     2701 tggccaagcg ctttggcgat gcctccaccg ccgccatcgg catggccaag accatcatga
     2761 accagtcgtt cgagagcgac gcgcgcaccc tggccgagat ggaggcctac gcccagtcca
     2821 tgtgccgcgg ctcgccctac caccaggaag cgatacgccg tttcaaggcg aaagaaccgc
     2881 tgcgcttcga ttgggatcgc gcaccatgag cgcagacctg gcccggcacc tgcagcagct
     2941 gatccgcccc ggcgacacgc tgtggtgggg ccaggccacg gccgagcccc tgacgctgac
     3001 gcgcgcgctg gtcgaacacc ggcacgccat tgcgcagggc gggcgcctcg gcgtattcgt
     3061 gggcatggcc gcgtccgaca cgctccggcc cgagcaggcc gacgtgctcg acttcttcgg
     3121 ctacgccgcc ggtggcgcgc accgccagct ggccgacgcg ggcaggctcg acatcctgcc
     3181 cagccactac tcgcatctgc cgggcctgat acagggtggc gtgctgccgg ccgatgtgct
     3241 gctgctgcag gtatcaaagc ccgacgcagc gggccggcac agcctgggcc tggtgcgcga
     3301 atacctgccc gccgtgctcg ccaaggcgcg cgtggtgata gccgaggtga atccctccat
     3361 tccctggacg catggcggcc cgtatctgca ggccggcgac ttcgccctgc tggtggacgc
     3421 cgagcatctg ccgctggatc agccgcgcgg cgccataggc gccaccgagc agcagatcgc
     3481 gcgccatatc gccggcctga tcgaggacgg cgccacgctg cagctgggcg tgggcaacct
     3541 gcccgaggcc gtgctggccg cgttgcacgg ccaccgcgat ctgggtctgc acacgggcgc
     3601 ggtgacggac ggcattgccc agctggccca ggcgggcgtg ctgaccaacg cccagaaaag
     3661 catcgaccgg ggcgtgggca ttggcggcat cctgatgggc agcgacatcc tgcgccgctg
     3721 ggccgagtgc aatccgcagc tggagctgcg cgccaccgac tacacgcacg accccgtggt
     3781 gctggcgaag agccacaaac tggccgccat caacgccgcc atcgaggtgg acctgaccgg
     3841 ccagatcaac gccgaggtgg cggccggctg ctatgtgggt gccgtgggcg gcgcggtgga
     3901 ttttttgcgc ggcgcggcca ggagttacgg cggcctgccc atcgtcgcgc tgccggccac
     3961 ggccaagggc gcgagccgca tcgtcgcgca gctctcgggc ccggtgagca cgccgcgcag
     4021 cgatgccggc ctcatcgtca ccgagcatgg cgtggccgac ctgcgcggcc agccgctgtc
     4081 gcgccgcgtg cgccggctga tagccatcgc cgcgcccgaa catcgcgaga gcctggaacg
     4141 ccaggcccat gaactgctgc gccgctgcgg cgcagtgctt taaaaaagat agctgttagc
     4201 gcttgcggga taagcgctag cggccaattt cattcaaatc ccaaggagaa acaccatgcg
     4261 ccgagccgcc atcgtcaccc cccttcgcac tcccgtcggc acctttggcg gcagcctgcg
     4321 cccggtgtct gtcgaggagc tggccgccac cacggtgcgc gccgtggtgc aaaaaagcgg
     4381 catagacccc acgcgcatcg acgatgtggt gtttgcccag tcctacgcca gcagtgagac
     4441 gccctgcgtc ggccgctggg ccgcgctgca ggcggggctg cccgtcagcg ttcccggcat
     4501 gcagctcgac aggcgctgcg gcggcggcct gcaggccatc gtcacggcgg ccatgatggt
     4561 gcagagcggc gccgccgacg tggtgattgc cggcggcgtg gagagcatga gcaacatcga
     4621 gtactacagc accgacatgc gctggggcgc acgctcgggc aacgtgcgct tctacgaccg
     4681 gctggatcgt gggcgcgagc gctcgcagcc cgtggagcgc ttcggcaaga tctccgggat
     4741 gatagagacg gccgagaacc tggcgcgtga ctaccagatc acgcgcgagc aggccgacgc
     4801 ctttgcgctg cgcagccacc agaaggcggc cgccgcctgg gcggcggggc gctttgccga
     4861 cgaaatcgtc ccggtgcagg tgccgcagcg caagggcgat cccatcctgt tcagccagga
     4921 cgagggtttc cgcgccgacg ccacgcctga gagcctgggc aagctgcgcg cgttgatgcc
     4981 gaacggcacg gtgacggccg gcaacgccag ccagcaaaac gatgccagcg ccgcctgcct
     5041 gatcgtcgcc gaggacaagc tggccgagct gggcctgacc cccatggcca ccctggttgg
     5101 ctgggcagcg gccggttgcg agccatccca catgggcata ggcccggtgc cggcggtgca
     5161 gaaactgctg gcgcggctga acctgaagat cgacgacatg gacctggtcg aactcaacga
     5221 ggccttcgcc tgccaggtgc tggccgtgct caagggctgg ggctggcagg accaggacgc
     5281 catcgcacac aagctcaacg tcaacggctc gggcatctcc ctgggccacc ccatcggcgc
     5341 caccggcgtg cgcatcctgg ccacgctgct gcacgagctg cagcgccgca agggccgcta
     5401 cggcctggag accatgtgca tcggcggcgg ccagggcatc gccgcggtgt tcgagcgggt
     5461 gtgacgcctg ccaacgggta aaccccggct ttggcaaaag cagattccca cggcgcggct
     5521 tgcgggccac catcgccgca ttccattcca cacctatagg agacacacca tgccatcttg
     5581 ccgccgcctc gccatcgccg cactggccct ggcgcccctg gccctcgcca gtacgccggc
     5641 cgctgcccag gacggccagc ccgtgcgcct catcgtcggc tatgccgcgg gcggcccggt
     5701 ggatcaggcc gcgcgcctgt ttgcgcccct gctgggcaag gaactgggcc agtccgtcat
     5761 cgtggagaac aagaccggcg ccaacgccac cattgccgga tccgaggtgg tgcgcgccaa
     5821 gcccgatggc ctgacgctgt ggtacgccgc cagccccacc atcaccatca gccccaacgt
     5881 gatgagcaag atgccgttcg acccggccaa ggacctgacg cccgtggcgc ccatcctgag
     5941 ctactacaac gtgctggtgg tgaacaacaa cgagccctac aagagcgtgc gcgagttggt
     6001 ggccaccgcc aagtccaatc cgggcaagct ggcctacggc tcggccggtg tgggcggctc
     6061 caaccatctg ggcgcgctgc tgttcgccaa gcgcagcggc atcgagatga accacatccc
     6121 ctacaagggc aacgccccgg cgatgacgga cgtgatcggc gggcagctca acatgatgct
     6181 ggacatcatc agcaccgcca gcgcctacat ccacagcggc aaggtgcgcg ccattgccgt
     6241 cacctcgccg cagcgcaatg cctcgctgcc ggacgtgccc acgttcgccg agtcgggcat
     6301 cgacggcctc aagggcttcg acgtgggtgg ttggtacggc gtctatggcc ccaagggcat
     6361 gtcgcccgag ctggtggcca agctcaacaa ggccgtcaat gccgcgctgc agcaacccga
     6421 gctgaagaag cgctacaagg aactgggcta cgacgagtgg acgggcgatg cgcacaagct
     6481 ggccgagcgc gccgccaagg agcgcgccat gtgggccacg gtgacgcagg gcatcaccgt
     6541 cgattgacat ggcagcgcgc attcatcaac tgctggatcg ctggctggct caggcgcccg
     6601 atcggccctt catacacctg ccggatggcc gcagcctgag ctttgccgac ctgggcgcgc
     6661 tgaccgacgc ggccgaggcc gagctgcgcc agctcggcgt gcgcgccggc gaccgcgtgc
     6721 tggtggtggc cgagaactgc cccgagcatg ccgcgctgat cctggccgcg agccgcgtcg
     6781 gcgcctggtc ctgcggcgtg aatgcgcgca tggcgccggg cgaggtcgat gcctttgccg
     6841 ccaaggccga tgcgcgcctg ctgtacttca cggccagcgg ctcgcgcgcc gccgccgggc
     6901 atgcgcgccg ccatgcgggc gtggccagcg tgttacccgg catgcagcac ggcgccgtgc
     6961 gcgagcaggc cacggtggag ccgccgccgc tacaggacga cgtggcggcg ctgatcttca
     7021 cctcgggcac caccggggat ccgaagggcg tgatggtcac gcatgagggc ttgatccact
     7081 tcgcccgcat ctcggcgcaa tcgcgcgcgc tgggcccgcg ggaccgcagc tacgccttcg
     7141 tgcccatgac gcatatcttc ggcctggcca cggtgctgct ggcctcgctc tgggccggcg
     7201 cgcagctggt gctgcgcccg cagttcgacc cggccgagct gctggacgcg ctggcgcacc
     7261 acggcgtgac gcagctgcag ggcccgccgg cgctgttctc gcgcctgctg gggtacttgc
     7321 acgagcaggg catggagcac cccgaggcgc cggcgctgcg ctacgtctac accggcgccg
     7381 ggccgctgga tctgacgctg aaacaccgcg tcgaggccac cttcggccag gcgctgcacc
     7441 atggctacgg cctgtcggaa tacgccggct cggtgcatct gacgcgcatg ggcgagcagc
     7501 gctctgacac cagcgtgggc tatgtggtcg aggatgccga ggtgcaggtc accgacccgg
     7561 ccacgggcca ggcgctacca acgggcgagc gcggcgagct gtggatacgc gggcgcggcc
     7621 tgatgcgcgg ctactttcgt gacccggccg ccaccgccgc ggtgatgaag gcgggcggct
     7681 ggtacgccag cggcgacctg ggcgagctgc atgccgatgg cgcgctgttc gtcgtcgggc
     7741 gcctgaagga gatgatcatc cgctcgggct tcaacgtcta tccggccgag gtcgagctgg
     7801 ctttgaacca gctgcccggc gtgcagcgca gcgccgtcgt cggccgccca gaaaaggacg
     7861 gcaacgagga agtcatcgct ttcgttgaac tatcgcccgg cgccagtttt gacgagaccg
     7921 ccgcccgcgc ccggctgcgc gagcagctgg caccctacaa gctgcccagc cacatcgtgc
     7981 ccgtggccga gctgcccacc agtaccaacg gcaaggtgct caagcgccag ttgcaagagc
     8041 aggccgccac cctttagcgt ctaccccgtc attcccgcgc aggcgggaat ccaggaacgc
     8101 ctcgtcacgg gcatggatcc ccgcctgcgc ggggatgacg ctgttcatac ctcggagacc
     8161 catatgacca cccaccgcat cacccgccgc caactcgttg ccgccggcct gctggccgcc
     8221 gccacacaac ccttcgccgc cctggcgaat gacgcctggc ccgccaagcc ggtgcgcttc
     8281 atcgtgccct tcccgcccgg cggcccggtg gacaccacgg cccgcgccgc cagcctgaag
     8341 ctgtccgaga tctggaagac ccccacggtg atcgacaacc gtgccggcgc cggcggcgtg
     8401 gtcggcgccc aggccgcggc gcgcgaggca ccggacggct acaccctgtt catgggcgcc
     8461 atccaccatg cggtcaaccc cacgctgatg ggcaacctgc cgtatgacat cgaaaaggac
     8521 ttcgccccgg tcagcttcgg cgccatgttc ccggtgttcg tcgtggtgca cccctcggta
     8581 ccggccaata ccctgcagga gctgatcgcc ctggcgaaga agcctggcag caacctcagc
     8641 tacgcctcat cgggcaacgg cggcggcacg cacctggcgg gcgagctgtt caacatggag
     8701 gcgggtacca aactgcaaca tgtgccctac aagggcagcg ccccggccat gaacgacctg
     8761 ctgggcggcc aggtgcagat catgttcagc gacgcgccca cggcgctgcc gcacatcaag
     8821 agcggccgcg tcaaggtgct ggccgtggcc agccgcaagc gctcggccat gctgcccgat
     8881 gtgccggccg ccgccgaggc cggcctgccc aactacgagg cttattcctg gacggcgctg
     8941 ttcgccccgg ccaagacacc gcaggccgtg ctggacaaga tcaacgccga cttcaacact
     9001 gccttcaagg atccggccgt gcgccagcga ctgctggacg gcggcgccga gacggccccc
     9061 ggcacgcagc aggagatgcg cgactttctg cgcgccgaga tcgccaaatg ggccaaggtg
     9121 atcaagacgg cggggatcac cacggggtag gaatatgatt gaaagtagct gctagcgctt
     9181 gtgtgacaaa cgctagcagc tactttattt gtagtaatgg attgtcggcg cgtcaatcct
     9241 cgctgatcag cttgatgctg caatattcct gcagcccctc gatgccgcat tccgaacccc
     9301 agccgctttc cttgacgccg ccaaacggcg cctcgggcat ggagacatgc aggctgttga
     9361 tgcccagcat gccggtctgc agaccgcgtg ccagatgcat gcgcgtggcg ctgtcgtggg
     9421 tgaaggcgta ggcggccagg ccatagggca gacggttggc gcgctccagg gcttcatcca
     9481 cgctgtgcgc gcggttgatc agcgccagtg ggccgaaggg ctcttcgttc atcacgcgcg
     9541 cgtcctcggg tacgtcggtg agcaccgtgg gttgccagaa ataacccggc ccggccatgg
     9601 catcgccacc gcattgcaac tgtgcgccgc gctccacggc gtcggcaatc aggctgcgca
     9661 tggcctgcac gcggcgcggg ttggccagcg ggcccatgct gctgccgtcc agcaggccgt
     9721 tgccgggtgt cagctggcgt gcgcgctcca ggaaggcgtg cacgaaggcc tcgtagaccg
     9781 gctccagcac atagatgcgt gtgggggcga tgcacacctg gccggcattg cggaacttgc
     9841 cggcgatgag catgtcggcg gcgcgctcca cctcggcgtc ggcgcagacg atggccgggg
     9901 cgtggccgcc cagctccatg gtggcgcgct tgaggctgcg ggcggcctgc tgcgccagct
     9961 gcttgcccac ggccgtggag ccggtgaagg agaacttggc gacgatgggg ctgtccagca
    10021 ggtaggtaga cacctcggcg ggcacgccga acacggcgtt cagcacgccc gcaggcaggc
    10081 ccgcgtcgtg cagcgcgcgc accagggcca ggccggtggc cggggtttcc tcggccagct
    10141 tgatgatcag cgtgcagccg gcggccaggg cgccggccat cttgcgcgcg ggggtgatgg
    10201 ccgggaagtt ccagggcgtg aacgccgcgc acacgcccac cggctcgtgc aacacggtga
    10261 tgcgctggtg caggtggcgc cccggcacca cgcggccgta gttgcgccgg ccctcttcgg
    10321 catgccaatc gaaggtgtcg gcgctggcca gcagctccag gcgcgcc
//
LOCUS       DAOMTQ010000109        10228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1290_length_10228_cov_25.001966, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000109 DAOMTQ010000000
VERSION     DAOMTQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_10228_cov_25.001966"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PK518_10840"
     CDS             <1..962
                     /locus_tag="PK518_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM49455.1"
                     /translation="IQFVPGGPVEQYLAEAKAGAAGAGGGGEGGGMAYRGAQGVDPKR
                     LEQIKALYGFDKPAHERFIQMLGQFARFDLGRSFFQNKDVWQLVKEKLPVSISLGLWT
                     FFISYLVAVPLGVAKAVRAGSRFDLVTTLIILVGYAIPGFVLGVALLVVFGGQLQWFP
                     LRGLTSSNWEQLSLMGKVTDYLWHITLPITAMVAGSFAVTAMLTKNAFLEEIRKQYVL
                     TARAKGLTERQVLWGHVFRNALIPIITGFPSAFIGAFFAGSLLIETLFSLDGLGLLSY
                     ESVIRRDYPVVLGTLYLFTLIGLVTKLISDLCYVWVDPRVKFD"
     gene            1030..1320
                     /locus_tag="PK518_10845"
     CDS             1030..1320
                     /locus_tag="PK518_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM49456.1"
                     /translation="MTWNIASAKQQFSEVVRLTAEEPQAIYNRDKPVAVLISAQEYEE
                     FKAWQAQKKRPTLAEQFDEIRALLAARGEDGIELPPRTTRYNPMVDDPHYWD"
     gene            1335..1739
                     /locus_tag="PK518_10850"
     CDS             1335..1739
                     /locus_tag="PK518_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRM49457.1"
                     /translation="MQLADTNVVSELMRKRPDAGALAWREMVDATYGKVTISAVTVDE
                     IMYGLARKQDAGLQAWFDGFIGRNEVLPVTQTIAYRAGEMRAGFSRRGMVRSQADMLI
                     AATAQIHALTLVTRNVRDFDGCGIGLLNPFTA"
     gene            1736..2776
                     /locus_tag="PK518_10855"
     CDS             1736..2776
                     /locus_tag="PK518_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49458.1"
                     /translation="MSAASPSLSPGRRAWRRFRANRLGFWSLVLFCTLVVISLAAELV
                     SNDRPLIVRYQGQTYFPMLKDYPETTFGGDFQTPADYLDPYVRRRITEGGNWALYTLN
                     PYGANTLNYFAKQPNPSGPSRDNWLGTDDRGRDLLAQLIYGFRVSVLFGMALTVVGVA
                     LGVAAGAVQGFFGGKTDLAFQRFIEIWGSMPELYLLIIFSAVFAPSIALLLVLLSLFG
                     WMGLSDYVRAEFLRNRQLDYVKAARALGVKSGHIIWRHILPNSMTPVVTFLPFRMGAA
                     ILALTSLDFLGLGVPPGTPSLGELLSQGKNNIDAWWISIFTFAVLVTTLLLLTFMGDA
                     LRDALDPRKGEH"
     gene            2778..4415
                     /locus_tag="PK518_10860"
     CDS             2778..4415
                     /locus_tag="PK518_10860"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRM49459.1"
                     /translation="MRATAPMNPQSPPLLQVRDLEVRFGAKQVVHGVSFDIAPGEKLA
                     LVGESGSGKTVTALALLRLAGDAQLTGSALMAGRDLLQLSERELRGVRGGDIAMVFQE
                     PMTALNPLMPVGEQVAEVLMLKQALTKAQSAQSAIELLASTGIPEPARRAGSFPHQLS
                     GGQRQRAMIAMALASRPKLLLADEPTTALDVTLRGQILDLLSDLQRQTGMAVLLITHD
                     LLLVRRFADRVAVMEQGVLVEQGAVAQVFGTPQHDYTRRLIGSTPQRDVIEGAAAEGA
                     TPAAQADALRVAYPVPLPGFRGWFAKGEFVAVNGVGFRLQRARTLGVVGESGSGKSTL
                     AQALLGLLPSTGELQVAGQAWQQPSHGNSSHNQALRQQVQVVFQDPFSSLSPRMTVEE
                     IVGEGLQVHAPQLPLPERRARVAAMLAEVGLTEAQFPGLLARYPHEFSGGQRQRLAIA
                     RALIVQPQLLVLDEPTSALDVTIQQQVLSLLQRLQKEHALSYLLITHDVAVVRAMAHE
                     VIVMKDGEVVESGSVQQVLDAPQHPYTQRLVEAARAP"
     gene            4659..6578
                     /gene="thrS"
                     /locus_tag="PK518_10865"
     CDS             4659..6578
                     /gene="thrS"
                     /locus_tag="PK518_10865"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRM49460.1"
                     /translation="MLHITLPDGSQREFPGPVTVAEVAASIGAGLAKAALAGKIGDRV
                     VDTSHLITEDSPLAIVTAKDADGLEVIRHSTAHLLAYAVKELFPDAQVTIGPVIDNGF
                     YYDFAYKRPFTPEDLVAIEKRMAELAAKDEPVVRRVLPRDEAVAYFKGLGEHYKAEII
                     ASIPSNEDVSLYREGAFEDLCRGPHVPSTGKLKHFKLMKVAGAYWRGDHRNEMLQRVY
                     GTAWASKDELQNYLHMLEEAEKRDHRKLGRELDLFHIDEHSPGTVFWHPKGWTLWQQV
                     EQYMRRVYQDNGYQEVKGPQILDKTLWEKTGHWDKYRENMFTTESEKRDYALKPMNCP
                     GHLLIFKQGIKSYRDLPLRYGEFGQCHRNEPTGGLHGIMRVRGFTQDDGHIFCTEDQI
                     QPEVLAFTQLLQKVYADFGYSDIIYKVATRPEARIGSDESWDKAEAALIHSLEASGCA
                     FEISPGEGAFYGPKIEYTLKDAIGRHWQCGTIQVDFSMPERLDAEYVGEDGGRHRPVM
                     LHRAIVGSLERFIGILIEQHAGALPVWLAPLQVAVLNITDAQQDYCREIAAKLQKALP
                     HQELRVVTDLRNEKITYKIREHALQKLPYILVAGDKEKAAGAVAVRARGNKDLGVMSL
                     DAFVELIARDIASKA"
     gene            6659..7273
                     /gene="infC"
                     /locus_tag="PK518_10870"
     CDS             6659..7273
                     /gene="infC"
                     /locus_tag="PK518_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRM49461.1"
                     /translation="MKAIATEFRDRRQREERKHRLNREITAPEVRLSGPENEPIGIVS
                     LQEALRMAGDLDVDLVEIAATASPPVCRLMDYGKFKYQEQKKAAEAKAKQTVIDIKEV
                     KFRPGTDDGDYNIKLRNIRRFLADGDKCKITLRFRGREITHQELGLALLNRLRDDLAD
                     TILVEQFPKLEGRQMIMMIAPARKKPGVKPAADAAPVASGSEAA"
     gene            7449..7652
                     /gene="rpmI"
                     /locus_tag="PK518_10875"
     CDS             7449..7652
                     /gene="rpmI"
                     /locus_tag="PK518_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRM49462.1"
                     /translation="MPKMKTKSSAKKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRQL
                     RGIVNVHDGDMGSIAKMLPMAGL"
     gene            7679..8038
                     /gene="rplT"
                     /locus_tag="PK518_10880"
     CDS             7679..8038
                     /gene="rplT"
                     /locus_tag="PK518_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRM49463.1"
                     /translation="MPRVKRGVTARARHKKVLALAKGFRGRRGNVFRIAKQAVMKAGQ
                     YAYRDRRNKKRVFRRLWITRINAAARELGLTYSQFTNGLKKAAIEIDRKMLADLAVHD
                     KAAFGSIVEQVKAKLAA"
     gene            8140..9192
                     /gene="pheS"
                     /locus_tag="PK518_10885"
     CDS             8140..9192
                     /gene="pheS"
                     /locus_tag="PK518_10885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRM49464.1"
                     /translation="MNELDSLVTSASEAFAHAATPADLENAKALFLGKSGRLTELMKG
                     MAQLSPEDKKTRGAAINVAKQAVEAALTARRQALAQAELERQLQAEALDVTLPGRKQG
                     RGGLHPVSLTLERIEGIFGSMGFDVADGPEIETDWFNFTALNTPEDHPARSMHDTFYV
                     EGGTATAPNLLRTHTSPMQIRHAVQHVKKYRDLIDAGQAMPEIRVIAPGRTYRVDSDA
                     THSPMFHQCEGLWIGENVSFKDLKVIFTAFCKTFFESDDLVLRFRPSFFPFTEPSAEI
                     DIQFQSGPLAGRWLEVAGSGQVHPNVVRNMGLDPERFIGFAFGMGPDRLTMLRYGVND
                     LRLFFDGDIRFLSQFQ"
     gene            9313..>10228
                     /locus_tag="PK518_10890"
     CDS             9313..>10228
                     /locus_tag="PK518_10890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM49465.1"
                     /translation="MQFPESWLREFCNPALSTQELADTLTMAGLEVEELAPVAPPFTK
                     IVVGEIKEAVQHPDADRLRVCQVDVGQGALLNIVCGAPNARVGIKVPCAMVGAELPPG
                     EDGKPFLIKVGKLRGVESYGMLCSARELKLSEDHGGLLELPADAPLGQDIRDYLKLDD
                     TLFTLKLTPNLAHCLSVYGIARELSALTGAPLAQPQFPQAVTSIADRVAVRISAPDLC
                     GRFSGRVIRNVNTRAATPQWMVDRLARCGQRSVSPLVDISNYVMFEFGRPSHIFDLDK
                     IHGGLDVRWGRAGEQLKLLNGNTVTLDEK"
BASE COUNT         1895 a         3291 c         3250 g         1792 t
ORIGIN      
        1 tcatccagtt cgtgcccggc ggcccggtgg agcagtacct ggccgaggcc aaggcgggcg
       61 ccgccggtgc aggggggggc ggcgagggcg gcggcatggc ctaccgcggc gcgcagggcg
      121 tggaccccaa gcgcctggag cagatcaagg cgctgtacgg cttcgacaag ccggcgcacg
      181 agcgctttat ccagatgctg gggcagttcg cgcgcttcga cctggggcgc agcttttttc
      241 agaacaagga cgtgtggcag ctggtcaagg aaaagctgcc cgtgtccatc agcctggggc
      301 tgtggacctt tttcatcagt tacctggtcg ccgtgccgct gggcgtggcc aaggcggtgc
      361 gcgcgggctc gcgctttgac ctggtgacca cgctcatcat cctggtcggc tatgcgattc
      421 cgggctttgt gctaggcgtg gcgctgctgg tggtgtttgg cgggcagctg cagtggtttc
      481 cgctgcgcgg gctgacgtca agcaactggg agcaactgag cctgatgggt aaggtgaccg
      541 actacctctg gcacatcacc ctgcccatca ccgccatggt ggcgggcagc tttgccgtga
      601 cggccatgct gaccaagaac gcgtttctgg aggagatccg caagcaatac gtgctcacgg
      661 cgcgcgccaa gggcctcacc gaacgccagg tgctctgggg ccatgtgttc cgcaacgcgc
      721 tgatccccat catcaccggc tttccctcag ccttcattgg cgcgttcttc gcgggttcgc
      781 tgctcatcga gacgctgttc tcgcttgacg gcttgggcct gctcagctac gagagcgtga
      841 tccgccgcga ctaccccgtg gtgctgggca cgctgtattt gttcacgctg atcggcctgg
      901 tgaccaagct gatctccgac ctgtgctacg tgtgggtcga tccgcgcgtg aagttcgatt
      961 gatgcctggg ttaaaaatgg acataattta tatccaactt gtccatcatt catcgtctgg
     1021 aggtgcatca tgacctggaa catcgccagt gccaaacagc agttctccga ggtcgtgcgc
     1081 ctgacagccg aggagccgca ggccatctac aaccgcgaca aacccgtggc ggtgctgatc
     1141 tcggcgcagg agtacgagga gttcaaggcc tggcaggcgc agaaaaaacg ccccacgctg
     1201 gccgagcagt tcgacgagat ccgggcgctg ctggccgcgc ggggcgagga cggtatcgaa
     1261 ctgccgccgc gcaccacccg ctacaacccc atggtcgatg acccgcatta ctgggattga
     1321 tggggacgct ggcaatgcaa ttggccgata ccaatgtcgt ttctgaactc atgcgcaaac
     1381 gcccggatgc gggcgcgctg gcgtggcgcg agatggtcga tgccacctat ggaaaagtga
     1441 ccatttctgc ggtgaccgtc gatgaaatca tgtacggcct ggcgcgcaag caggatgcgg
     1501 gtttgcaggc ctggtttgac ggcttcatcg gccgcaacga ggtactgccc gtgacgcaga
     1561 ccattgccta tcgcgccggc gagatgcgtg ccggcttttc ccggcgtggc atggtgcgta
     1621 gccaggccga catgctcatc gccgccaccg cccagatcca cgccctgacc ctggtcacgc
     1681 gcaatgtgcg cgactttgac ggctgcggca taggcctgct caaccccttt accgcatgag
     1741 cgccgcaagc ccatccttgt cccccggccg ccgcgcctgg cggcgtttcc gtgccaaccg
     1801 cctgggcttc tggagcctgg tgctgttctg caccctggtc gtcatcagcc tggctgccga
     1861 gctggtcagc aacgaccggc cgctcatcgt gcgctaccag gggcagacct acttccccat
     1921 gctcaaggac taccccgaga ccacgttcgg tggcgacttc cagacgcccg ccgactacct
     1981 cgacccctat gtgcgccggc gcatcaccga gggcggcaac tgggcgctgt acacgctcaa
     2041 cccctacggc gccaataccc tgaactactt cgccaagcag cccaacccct cgggcccgtc
     2101 gcgcgataac tggctgggca cggacgaccg cgggcgcgac ctgctggcgc agctgatcta
     2161 cggctttcgc gtcagcgtgc tgttcggcat ggcgctcacc gtggtcggcg tggcgctggg
     2221 cgtggcagcc ggcgccgtgc agggcttctt tggcggcaag accgacctgg ccttccagcg
     2281 cttcatcgag atctggggct ccatgccgga gctgtacctg ctcatcatct tcagtgcggt
     2341 gtttgcgccc agcattgcgc tgctgctggt gctgctctcc ctgttcggct ggatggggct
     2401 gtcggactat gtgcgcgccg agtttctgcg caaccgccag ctcgactacg tgaaggccgc
     2461 gcgcgccctg ggcgtcaaaa gcggccacat catctggcgc cacatactac ccaacagcat
     2521 gacgccggtg gtcacctttc tgccctttcg catgggtgcg gccattttgg cgctgacctc
     2581 gcttgatttt ctgggcttgg gcgtgccgcc gggcacaccc agcctgggcg agctgctatc
     2641 gcagggcaag aacaacatcg acgcctggtg gatctccatc ttcaccttcg ccgtgctggt
     2701 gaccacgctg ctgctgctca ccttcatggg cgacgcgctg cgcgacgccc tcgacccccg
     2761 caagggggag cactgacatg cgcgccactg cccccatgaa ccctcagtcg cccccgctct
     2821 tgcaagtacg ggatctggag gtgcgctttg gcgccaagca ggtggtgcac ggcgtgagct
     2881 ttgacatcgc ccccggcgag aagctggccc tggtgggcga gtcgggttcg ggcaagaccg
     2941 tgacggcgct ggccctgctg cgcctggccg gcgacgccca gctgacgggc agcgcgctga
     3001 tggccgggcg cgatctgctg caactgagcg aacgcgagtt gcgcggcgtg cgcggcggcg
     3061 acatcgccat ggtgttccag gagcccatga ccgcgctgaa cccgctcatg cccgtgggcg
     3121 agcaggtggc cgaggttttg atgctgaaac aggccctgac caaggcacag agcgcgcaat
     3181 cagctatcga attgttagca agcacgggca tccccgagcc cgcgcgacgc gccggcagct
     3241 tcccgcacca gctctcgggc gggcagcgcc agcgcgccat gatcgccatg gccctcgcct
     3301 cgcggcccaa gctgttgctg gccgacgagc cgacgacggc gctcgacgtc acgctgcgcg
     3361 gccagatcct cgatctgttg tccgacctgc agcgccagac cggcatggcc gtgctgctca
     3421 tcacgcacga cctgctgctg gtgcgccgct ttgccgaccg cgtggcggtg atggagcagg
     3481 gcgtgctggt ggagcagggc gcggtggcgc aggtgtttgg taccccgcaa catgactaca
     3541 cgcgccgcct gatcggcagc acgccgcagc gcgacgtgat cgagggtgcg gcggccgaag
     3601 gcgccacccc cgccgcccag gccgatgcac tgcgcgttgc ctatcccgtg ccactgccgg
     3661 gttttcgtgg ctggtttgcc aagggcgagt tcgtggcggt caacggcgtg ggttttcgcc
     3721 tgcagcgcgc gcgcaccctg ggcgtggtgg gcgagtcggg ctcgggcaag tccacgctgg
     3781 cccaggccct gctgggcctg ttgccgtcaa cgggtgagtt gcaggtggcg ggccaggcct
     3841 ggcagcagcc gtcgcatggc aacagctccc ataaccaggc cttgcgccag caggtgcagg
     3901 tggtgttcca ggacccgttc tcgtcgctgt cgccgcgcat gaccgtggaa gagatcgtcg
     3961 gcgagggcct gcaggtgcac gcaccccagc tgccgctgcc tgaacgccgc gcgcgcgtgg
     4021 ccgccatgct ggccgaggtg ggcctgaccg aggcgcagtt tcccggcctg ctcgcgcgct
     4081 acccgcatga attctcgggc ggccaacgcc agcgcctggc gatcgcgcgc gccctcatcg
     4141 tgcagccgca gctgctggtg ctggacgagc ccaccagcgc gctggacgtg accatccagc
     4201 agcaggtgct gtcgctgctg cagcggctgc aaaaggagca cgccctgtcc tacctgctca
     4261 ttacccatga tgtggccgtg gtgcgcgcca tggcgcatga ggtcatcgtc atgaaggacg
     4321 gcgaggtggt ggagtccggc agcgtgcagc aggtgctgga tgcgccgcag cacccctaca
     4381 cgcagcgcct ggtggaggcg gcgcgcgcgc cgtgagcgca gctccaggcc actgccggct
     4441 agaatatgcg ctttacaaga taacaagggc gagaaaggca atgtcggtta tgacaccgcg
     4501 aactacgacg gagacctcca ccggccgcta aggctggccg ttcgcgcctg cacgagagct
     4561 tttctcaccc tcaccaaagc gcggactttt gtaccgcgct tttttgcttc tgggcctcaa
     4621 agcgcgcgcc gcaaccgcaa ttcccaagga tttctcacat gcttcacatc acgctgcccg
     4681 atggttccca acgcgaattc cccggcccgg tgaccgtggc cgaggtggcc gcctcgatcg
     4741 gcgctggcct ggccaaggca gccctcgccg gcaagatcgg tgacagggtg gtcgatacca
     4801 gccacctgat caccgaagac agcccgctgg ccatcgtcac cgccaaggat gccgatggcc
     4861 tggaggtcat tcgccactcc accgcccacc tgctggccta cgccgtcaaa gagctgttcc
     4921 cggacgcgca ggtcaccata ggcccggtga tagacaacgg cttttattac gactttgcgt
     4981 acaagcgccc gttcacgccc gaggatctgg tggcgatcga gaagcgcatg gccgagctgg
     5041 ccgccaagga cgaaccggtg gtgcgccgcg tgctgccgcg cgacgaagcc gtggcgtatt
     5101 tcaagggcct gggcgagcac tacaaggccg agatcattgc cagcatcccc agcaacgagg
     5161 atgtgagcct gtaccgtgaa ggcgcgtttg aagacctgtg ccgtggcccg cacgtgccca
     5221 gcaccggcaa gctcaagcac ttcaagctga tgaaggtggc cggggcctac tggcgtggcg
     5281 accatcgcaa cgagatgctg cagcgcgtct acggcacggc ctgggccagc aaggacgaac
     5341 tgcagaacta cctgcacatg ctggaagagg cagagaagcg cgaccatcgc aagctcgggc
     5401 gtgaactcga tctgttccac atcgacgagc attcgcccgg caccgtgttc tggcacccca
     5461 agggctggac gctttggcag caggtcgagc aatacatgcg ccgcgtctac caggacaacg
     5521 gttaccagga ggtcaagggt ccgcaaatcc tggacaagac cctgtgggag aagaccggcc
     5581 actgggacaa gtaccgcgag aacatgttca cgaccgagag tgaaaagcgc gactacgcct
     5641 tgaagcccat gaactgcccc ggccacctgc tcatcttcaa gcagggcatc aagagctacc
     5701 gcgacctgcc gctgcgctac ggcgagtttg gccaatgcca ccgcaacgag cccaccggcg
     5761 gcctgcacgg catcatgcgc gtgcgcggtt tcacgcagga cgatggccat atcttctgca
     5821 ccgaagacca gatccagccc gaggtgctgg ccttcacgca gttgctgcag aaggtctacg
     5881 ccgactttgg ctatagcgac atcatctaca aggtggctac gcgccccgag gcgcgcatcg
     5941 gctcggatga gagctgggac aaggccgagg cggcgttgat ccacagcctg gaggcgtcgg
     6001 gctgcgcgtt cgagatttcg cccggcgagg gcgccttcta cggcccgaag atcgaataca
     6061 ccttgaagga cgccattggc cggcactggc agtgcggcac gatacaggtg gacttttcca
     6121 tgcccgagcg cctggacgcc gaatacgtcg gcgaggatgg cggccgccac cgtccggtga
     6181 tgttgcaccg cgccatcgtt ggttcgctgg agcgcttcat cggcatcctg atcgagcagc
     6241 acgcgggcgc gctgccggtg tggctggcgc cgctgcaggt ggcggtgctc aacatcaccg
     6301 atgcacagca ggactactgt cgcgaaattg ccgcaaagct gcaaaaagca ctgccccatc
     6361 aagagcttag ggtggtgacc gatctgcgca acgaaaagat tacgtataaa atacgcgagc
     6421 atgcgctgca aaagctgccc tacatcctgg tcgccggcga caaggaaaag gccgccggag
     6481 ccgtcgcagt gcgcgcccgg ggcaacaaag acctgggtgt gatgtccctc gacgcctttg
     6541 tcgaactgat cgcacgggac atcgcctcca aagcctgatt tttgagaatc aaacatcgct
     6601 ttgaagcgca caggtggtgc gtgagcagct acgcttttcg tagctttttt gattgaaggt
     6661 gaaagccatc gctactgaat ttcgtgaccg tcgccagcgt gaggaacgca agcaccgtct
     6721 gaaccgtgaa atcacggcgc ctgaagtgcg cctgtctggt cctgaaaatg agcccatcgg
     6781 catcgtcagc ctgcaggaag ccctgcgcat ggcgggcgac ctggatgtgg atctggtgga
     6841 aatcgccgct acggccagtc ctcccgtctg ccgcttgatg gattacggca agttcaagta
     6901 ccaggagcag aagaaggcgg ccgaggccaa ggccaagcag acggtcatcg acatcaagga
     6961 agtgaaattc cgtccgggta cggatgatgg cgactacaac atcaagctgc gcaacatccg
     7021 ccgcttcctg gccgatggtg acaaatgcaa gatcacgctg cgctttcgtg gccgcgagat
     7081 cacgcatcag gaactaggcc tagcactgct caaccgcctg cgtgacgacc tggccgacac
     7141 cattttggtc gagcagttcc ccaagctcga aggccgtcag atgatcatga tgatcgcgcc
     7201 ggcgcgcaag aagcctgggg tcaagccggc ggcagatgcc gcgccggtcg ccagcggcag
     7261 cgaagcggcg taagcaaaga aaatttggcc ggcgtccatt gcgagatggg cgccagccga
     7321 taggcggtgc gagagcattg ccgcacggcg gactgtaaag cctgccgttt taaaaagtgg
     7381 ctcggggcca gcaaggatgc acagtggtgt gcagacgcct cacgagcaca aatcaaagga
     7441 gcatgcaaat gcccaaaatg aagaccaaga gcagcgcgaa gaagcgtttt cgcgttcgtc
     7501 cgggtggcac cgtcaagcgc ggtcaagcct tcaagcgtca catcctgacc aagaagacca
     7561 ccaagaacaa gcgccaactg cgtggcatcg tgaatgtgca cgacggtgac atgggctcca
     7621 tcgccaagat gctgcccatg gctggcctgt aatcaactga cgaacaagga gaaaacacat
     7681 gcctcgcgtc aaacgtggtg taacggcccg cgcccgtcac aaaaaggttc tcgccctggc
     7741 caagggtttc cgcggtcgcc gcggcaatgt cttccgcatc gccaagcagg cggtgatgaa
     7801 ggctggtcag tacgcttacc gcgatcgccg caacaagaag cgcgtgttcc gccgtctgtg
     7861 gatcacgcgt atcaacgctg ctgcgcgtga actgggcctg acctacagcc agttcaccaa
     7921 cggcctgaag aaggccgcca tcgagatcga ccgcaagatg ctggccgacc tcgcggtgca
     7981 cgacaaggct gcctttggca gcatcgtgga gcaagtcaag gccaagctgg ctgcttgagt
     8041 tcacgataga tagctacttg ttttgtagct gtctgcgcac aaacagcaag ggctagggct
     8101 tgaaaaggcg ctagcccttg ttttatttca agagtcgata tgaacgagct ggattccctg
     8161 gtgacaagcg cgagcgaagc cttcgcgcat gctgcaaccc ctgccgacct ggaaaacgcc
     8221 aaggcgctgt ttctgggcaa gtcgggccgc ctgactgaac tgatgaaggg catggcgcag
     8281 ctctcgcccg aggacaagaa gacgcgcggc gcagccatca acgtcgccaa gcaggctgtg
     8341 gaggccgcat tgacggcacg tcgccaggcc ctggcccagg ccgagctgga acgccaactc
     8401 caggccgagg cgttggacgt gacgctgccc ggccgcaagc aaggccgtgg cggtctgcac
     8461 ccggtgtcgc tgacgctgga gcgcatcgag ggcatcttcg gctccatggg ttttgacgtg
     8521 gccgacggcc ccgagatcga gaccgactgg ttcaacttca cggcactgaa cacgcccgag
     8581 gaccacccgg cgcgctccat gcacgacacc ttctacgtgg aaggaggcac cgccacagcg
     8641 cccaacctgc tgcgcacgca caccagcccg atgcagatcc gccatgccgt gcagcatgtg
     8701 aagaaatacc gcgacctgat tgacgccggc caggccatgc ccgagatccg cgtcatcgcg
     8761 cccggccgca cctaccgcgt cgacagcgac gccacgcact cgcccatgtt ccaccagtgc
     8821 gaaggcctgt ggattggcga gaacgtcagc ttcaaggacc tgaaggtcat cttcacggcc
     8881 ttctgcaaga ccttctttga aagcgatgac ctggtactgc gctttcgccc cagcttcttc
     8941 ccgttcaccg agcccagcgc cgagatcgac attcagttcc agagcgggcc gctggccggc
     9001 cgctggctgg aggtggccgg cagcggccag gtgcacccca acgtggtgcg caacatggga
     9061 ctggaccctg agcgcttcat cggctttgcc ttcggcatgg ggccggaccg cctgaccatg
     9121 ctgcgctatg gcgtgaacga tttgcgcctg ttcttcgacg gcgacatccg cttcctgtcg
     9181 cagttccagt aacgcaaaaa ggtgagctgt tcgcgcttgt cctgatcgga catcaacacg
     9241 ttttcatatt gaaacgcacg tatagcaagc gcaataagct catttttaaa gagcacgcca
     9301 aagaatctga ctatgcaatt tcccgaatcc tggttgcgcg aattctgcaa ccccgcactt
     9361 tccacgcaag agctggccga caccctgacc atggccggcc tggaggtcga agagctcgcc
     9421 cccgtggccc cgccgttcac aaagattgtc gtcggcgaga tcaaggaggc cgtgcagcac
     9481 cccgacgccg accgcctgcg cgtgtgccag gtggacgtgg ggcagggcgc cctgctgaac
     9541 atcgtctgcg gcgcgccgaa tgcacgcgtg ggcatcaagg tgccctgcgc catggtgggt
     9601 gccgagctgc cgccgggcga ggacggcaag ccctttttga tcaaggtggg caagctgcgt
     9661 ggcgtggaga gctacggcat gctgtgctcg gcgcgcgagc tcaagctgtc tgaagaccac
     9721 ggtggcctgc tcgagctgcc cgccgatgct ccgctgggcc aggacatccg tgactacctg
     9781 aagctcgacg acacgctgtt tacgttgaaa ctaacgccga acctggcgca ttgcctgagc
     9841 gtctatggca tcgcgcgcga actttcggcc ctgaccggcg ccccgctggc ccagccgcag
     9901 ttcccccagg ccgtcaccag cattgccgac cgcgtcgcgg tgcgcatcag cgcgcccgac
     9961 ctgtgcgggc gcttctccgg ccgcgtgatc cgcaacgtca acacccgcgc cgccacgccg
    10021 cagtggatgg ttgatcgcct ggcgcgctgc ggccagcgca gcgtctcgcc gctggtggat
    10081 atctccaatt acgtgatgtt cgagttcggc cggccgtcgc atatctttga cctggacaag
    10141 atccacggcg gcctggatgt gcgttggggc agggcgggcg agcagctcaa gctgctgaac
    10201 ggcaacaccg tcacgctgga cgaaaagg
//
LOCUS       DAOMTQ010000110         9972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1343_length_9972_cov_23.229102, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000110 DAOMTQ010000000
VERSION     DAOMTQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1343_length_9972_cov_23.229102"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="gspL"
                     /locus_tag="PK518_10895"
     CDS             complement(<1..679)
                     /gene="gspL"
                     /locus_tag="PK518_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HRM49466.1"
                     /translation="MSTLVITLPLGSPQAPADYSYTLTVDGHNATQQASASAALLPDP
                     GRTGEVVAVVPVRALSWQRVTLPPGALAQSARLRAVLDGLLEEQLLDDPAQLHFALQP
                     GAQAGSPIWVAVCDRAWLRQALQALEAAGRPADRVVPEFAPGPTASGREECEVLGQPE
                     DAYAVLTGHGAQQAVAVLPLAAARALFTQAPGDEDEDPLPVRAEPAVAALAERLLGRP
                     VQLRTASQ"
     gene            complement(676..1722)
                     /gene="gspK"
                     /locus_tag="PK518_10900"
     CDS             complement(676..1722)
                     /gene="gspK"
                     /locus_tag="PK518_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HRM49467.1"
                     /translation="MRGRARNAQRTTHNAQRASQAGAALLTAMLTVTLVATFAAAAMW
                     QQWRAIEVETAERARIQSAWILVGALDWSRLILREDSLAQGGDGTDNLSEPWAVPLQE
                     ARLSTFLAANRNVAQVEDASTDTADAFLSGQITDQQGLLNLRNLAGDKQVDATAQRQF
                     ARLFDYLGLPRQQLDQLAQAVLLATMREGGTDNTLLLPQSVAQLGWWGLPQQSVTALA
                     PYVTLLPVRTLVNLNTASAVVLWASADGLDMADAQRLVQRRETQYFRNPADAAALLRD
                     GSNAISASTHAVASSYFEVRGRLRLDGAVVEERSLVFKQRGDVRTLWRERGGLSALDD
                     TALGPKMPRPTSIP"
     gene            complement(1758..2438)
                     /locus_tag="PK518_10905"
     CDS             complement(1758..2438)
                     /locus_tag="PK518_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM49468.1"
                     /translation="MRRGQPGFTLVELLVAIAVMALLAIVSWRGLDGMVRAQQITRER
                     ADAVLELQTVLAQWGADLDALQAIEHTEPLAWDGQALRLTRRGMRQPDEGAIVVAWAL
                     RNADGTNRWLRWQSPPVRTRAEWSEAWARAAQWARTPSSAERERETLLMPLAGWQLYY
                     YRGGAWANAQSANTGASLPQAPGGLSSTAASIPEGVRLQLTLPEGGALAGVVTRDWAN
                     PLLGGGKS"
     gene            complement(2438..2797)
                     /gene="gspI"
                     /locus_tag="PK518_10910"
     CDS             complement(2438..2797)
                     /gene="gspI"
                     /locus_tag="PK518_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HRM49469.1"
                     /translation="MKDKAAGFTLVEVLVALAIVAIALMAGLQATSALTRNTARQTDV
                     VLAHACAENELVKVRLSRQMPAIGDARVVCEQAGRGYEVAVSVTPTPNPQFRRVDAQV
                     FDAQAPVLRLSTIVGRY"
     gene            complement(2794..3252)
                     /locus_tag="PK518_10915"
     CDS             complement(2794..3252)
                     /locus_tag="PK518_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM49470.1"
                     /translation="MPAGAAQEPPGRAVAQGGFTLLELLVVIAIIALATAGVGLALRD
                     SGAAALQREGERLAALLEAARAQSRASGAAVRWRADAQGFQFEGLVGSQLPRHWLDSA
                     TSVRGPATLWLGPEPLIGAQQVLIVSQAHPERAVRVATDGLRPFSAQALP"
     gene            complement(3298..3753)
                     /gene="gspG"
                     /locus_tag="PK518_10920"
     CDS             complement(3298..3753)
                     /gene="gspG"
                     /locus_tag="PK518_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRM49471.1"
                     /translation="MTPSSPPSFAQHARRRLAAGFTLIELMVVLVIIGVLAALIVPNV
                     LDRADDARVTAGRTDIANISQALKLYRLDNQRYPTAEQGLQALIAKPATAPVPGNWRP
                     YLEKLPNDPWGHPYQYLNPGIKGEVDVMSFGADGQSGGEGKNADIGSWQ"
     gene            3801..4235
                     /locus_tag="PK518_10925"
                     /pseudo
     CDS             3801..4235
                     /locus_tag="PK518_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
     gene            complement(4368..4958)
                     /locus_tag="PK518_10930"
     CDS             complement(4368..4958)
                     /locus_tag="PK518_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM49472.1"
                     /translation="MNGPRLWLVRHARPLVAPGLCYGRLDMAADASASRSAATALAHA
                     LPAKVAGVWHSPLQRCELLALELRALRPDLASNPEPRIAETDFGAWEGQAWSAIARAE
                     IDAWAANLAGHAPGGGESLAAMLQRVALALRDAAARAHDQGADMVWISHAGVARCVQW
                     LQQHGPGRAPQAHEWPLQAPDFGAWVSVALPTQGWA"
     gene            complement(4955..5767)
                     /locus_tag="PK518_10935"
     CDS             complement(4955..5767)
                     /locus_tag="PK518_10935"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRM49473.1"
                     /translation="MQALRHYLLAVQFFTRLPVTGRLAAWVGYSPAMLRAASAHFPGV
                     GWLVAAVAMGVYAALWWALGGQGFAPLVAAVGSTIATVLVTGGFHEDGLADVADGLGG
                     AYERERALTIMKDSRIGAFGAMALVLALGAKLALLAQLGSLGLEVALWALLGAHVLSR
                     LWPLFVVRALPHVGDTATSKSKPLADQITGRALAAAVLWCFLPLALMYQALGASVLIV
                     SVLCSALGGAWMLWRFARRLQGFTGDCLGATQQVAEIGFYLGAAMALAPRAA"
     gene            complement(5838..7403)
                     /gene="ilvA"
                     /locus_tag="PK518_10940"
     CDS             complement(5838..7403)
                     /gene="ilvA"
                     /locus_tag="PK518_10940"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HRM49474.1"
                     /translation="MTQPLTPLDYLKKILTARVYDVAVESDLQPARALSRRLHNKVLL
                     KREDQQPVFSFKLRGAYNKMAQLSAEQLAKGVICASAGNHAQGVAMSAARLGTRAVIV
                     MPTTTPQVKVDAVKTLGGEVVLMGDSYSDAYEHSLRLQQEQGLSFVHPFDDPDVIAGQ
                     GTIAMELLRQLQALGSHRLDAVFVPIGGGGLIAGVANYLKAVSPQTKVIGVQMNDSDA
                     MAQSARQHARVQLPDVGLFADGTAVKLVGEETFRVAHDLVDDYVIVDTDAVCAAIKDI
                     FADTRSIVEPSGALGVAAIKQYVAKHKTKGETYATILSGANMNFDRLRFVAERADVGE
                     EREALFAVTIPEERGSFKRFCEVVGALPGGPRNVTEFNYRISHERLAHVFVGLSTHGR
                     GESEKIARNFAKQGFESLDLTHDELAKEHLRHLVGGRSQLARDERLMRFTFPERPGAL
                     FKFLSLMQPTWNISLFHYRNQGADYGRILVGMQVPAEDAAAFDAFLQALGYPYVEETQ
                     NPAYRLFLKANGG"
     gene            7681..8130
                     /locus_tag="PK518_10945"
     CDS             7681..8130
                     /locus_tag="PK518_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRM49475.1"
                     /translation="MECTVSWTGNAGTRSGMGFIAETGSGHILAMDGAPDAERPESGG
                     QNLAPRPMEAVLAGTGGCTAYDVVLILKRGRHDVRGCSVRLTSERAATEPKVFTRIHM
                     HFTVTGRGIPASAVERAIAMSHEKYCSASIMLGKTAQITTGFEIVEG"
     gene            8410..9513
                     /locus_tag="PK518_10950"
     CDS             8410..9513
                     /locus_tag="PK518_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRM49476.1"
                     /translation="MKKSLIALAVLAASGAAMAQSSVTLYGVADAGVTYINGKDNWSG
                     VSSGNQLTSRIGFRGVEDLGGGLKANFRLEAGLNLDVGDGASGYAGAKAGEGLAFKRN
                     STVGLEGGFGEVRLGRELTAAYDAVARYDVFGSVGLAYSRMWVAGDTAESSTNATATA
                     PNIRVSNAVTYVSPNFAGFKVGVNYGFGEIAGKNSDARYLGAGLTYDNGPISVGLAAE
                     QLKQNTVGGNSSGSVNGDMTSWALGASYDFGVAKLLGAYRTSKHENWANNLIGNDNKR
                     NGYLLAVTAPVGPGLVKASYNRYENKLGGTTTKADQFGLGYVYSLSKRTSVYGTYAYL
                     KNKNGASLYTLGAGGLKNNGSQQGVQMGITHTF"
     gene            9728..>9972
                     /locus_tag="PK518_10955"
     CDS             9728..>9972
                     /locus_tag="PK518_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRM49477.1"
                     /translation="MRLLTKKNTVLAALALCATGAAMAQAANTSKVELWGIVDVAVRH
                     TNNEGAAKTGLTKMIGGGMSQSRWGINVEEDLGGGSKA"
BASE COUNT         1575 a         3439 c         3422 g         1536 t
ORIGIN      
        1 gctggctggc ggtgcgcagc tgcaccgggc ggcccagcag gcgctcggcc agcgcggcca
       61 cggcgggttc ggcgcgcacc ggcagggggt cttcatcttc atcgcccggt gcctgggtga
      121 agagcgccct ggcggccgcc agcggcagca cggccacggc ctgctgcgcg ccatggccgg
      181 tgagcacggc gtaggcgtct tcgggctggc ccagcacctc gcattcctcg cgtccgctgg
      241 ccgtggggcc gggagcgaac tccggcacca cgcgatcggc cgggcggccc gcggcctcca
      301 gcgcctgcag ggcctggcgc agccaggcgc ggtcgcacac ggcgacccag atcgggctgc
      361 cggcctgggc acccggctgc agggcaaagt gcagctgggc cgggtcgtcg agcagctgct
      421 cttccagcag gccgtcgagc acggcgcgca gccgcgcgct ttgcgccagc gcgcccgggg
      481 gcagcgtcac gcgctgccag gacagggcgc gcaccggcac cacggccacc acttcgccgg
      541 tgcggcccgg gtcgggcagc agggcggcgc tggcgctggc ttgctgggtg gcgttgtggc
      601 cgtcgaccgt gagcgtgtag ctgtagtcgg cgggggcctg cggcgagccc aggggcaggg
      661 tgatgacgag ggtgctcatg ggatggaggt cgggcggggc attttagggc ccagtgcggt
      721 gtcgtccagg gcgctcaggc cgccgcgctc gcgccacagg gtgcgcacgt cgccgcgctg
      781 cttgaagacc agggagcgct cctcgaccac ggcgccatcc aggcgcagcc gcccgcgcac
      841 ctcaaagtag ctggaggcca cggcgtgcgt gctggcgctg atggcgttgc tgccgtcgcg
      901 cagcagcgcg gccgcatcgg cgggattgcg aaagtattgc gtctcgcgcc tttgcaccag
      961 gcgctgggcg tcggccatgt ccaggccgtc ggcgctggcc cacagcacca cggcgctggc
     1021 cgtgttcagg ttgaccaggg tgcgcacggg cagcagcgtg acgtagggcg ccagcgccgt
     1081 gaccgactgc tgcggcagcc cccaccagcc cagctgggcc acgctttgcg gcagcagcag
     1141 cgtgttgtca gtgccgccct cgcgcatggt ggccagcagc accgcctgcg ccagctggtc
     1201 aagctgctgg cgcggcaggc ccaggtagtc gaacaggcgc gcgaactgcc gctgcgcggt
     1261 ggcgtccacc tgcttgtcgc cggccaggtt gcgcaggttc agcaggccct gctggtcggt
     1321 gatctggccg gacagaaagg cgtcggcggt gtcggtgctg gcatcctcca cctgggccac
     1381 gttgcggttg gcggccagaa aggtggacag gcgcgcctcc tgcagcggca cggcccaggg
     1441 ttcgctgagg ttatcggtgc cgtcgccgcc ctgcgccagc gagtcctcgc gcagtatcag
     1501 gcgcgaccag tccagcgcgc cgaccaggat ccaggccgac tggatgcgcg cgcgctcggc
     1561 ggtctcgacc tcgatagcgc gccactgctg ccacatggcc gcggcggcaa aggtggccac
     1621 cagggtcacg gtgagcatgg ccgtcagcag cgcggcgcct gcctggctgg cacgttgtgc
     1681 gttgtgcgtt gtgcgttgtg cgttgcgcgc gcgcccccgc aaaaccctgg acgcccatcg
     1741 ctcaacgttc cgggccctca tgacttgccc ccgcccagca gcgggttcgc ccagtcgcgc
     1801 gtgaccacgc cggccagcgc gccgccctcg ggcagcgtga gctgcaggcg cacgccctcg
     1861 ggaatgctgg ccgcggtgct gctcagcccg ccgggcgcct gcggcaggct ggcgccggtg
     1921 ttggccgact gtgcattggc ccaggcaccg ccgcggtagt aatacagctg ccagccggcc
     1981 agcggcatga gcagcgtttc gcgctcgcgc tcggcgctgc tgggcgtgcg cgcccactgc
     2041 gcggcgcggg cccaggcctc gctccattcg gcgcgcgtgc gcaccggggg cgactgccag
     2101 cgcagccagc ggttggtgcc gtccgcgtta cgcagcgccc aggcgacgac gatggcgccc
     2161 tcgtcgggct ggcgcatgcc gcggcgcgtc aggcgcagcg cctggccatc ccaggccagg
     2221 ggctcggtgt gctcgatggc ctgcagcgcg tccaggtcgg cgccccattg cgccagcacg
     2281 gtctgcagct ccagcacggc gtcggcgcgc tcgcgcgtga tctgctgcgc gcgcaccatg
     2341 ccgtccagcc cgcgccagct gacgatggcc agcagcgcca tcaccgcaat ggcgaccagc
     2401 agctcgacca gggtgaaacc gggctgcccg cgccgcatca ataccttccc acgatggtgg
     2461 acaggcgcag caccggcgcc tgtgcgtcaa acacctgcgc atccacgcgc cggaactgcg
     2521 ggttcggcgt gggtgtcacg ctcaccgcca cttcgtagcc gcgcccggcc tgctcgcaga
     2581 ccacgcgcgc gtcgccaatg gccggcatct ggcgcgacag gcgtactttg accagctcgt
     2641 tctcggcgca ggcatgggcc agcaccacgt cggtctggcg tgcggtgttg cgcgtcaggg
     2701 cggatgtggc ctgcagcccc gccatcaacg caatggccac gatggccagg gccaccagca
     2761 cctcgaccag cgtgaaccca gccgccttgt ctttcatggc agggcctgcg cgctgaaggg
     2821 ccgcagcccg tcggtggcca cgcgcacggc gcgctcgggg tgggcctggc tgacgatcag
     2881 cacctgctgc gcgcctatca gtggctcggg ccccagccac agcgttgccg ggccgcgcac
     2941 cgaggtggcg ctgtccagcc aatggcgcgg cagctggctg cccaccaggc cctcaaactg
     3001 aaagccctgg gcatcggcgc gccagcgcac ggccgcaccc gaggcgcgcg actgcgctcg
     3061 cgccgcctcc agcagcgccg ccaggcgctc gccctcgcgc tgcagcgccg cagcgccgct
     3121 gtcacgcagc gccaggccca cgccggccgt ggccagggcg atgatggcga tcaccaccag
     3181 cagctcaagc agtgtgaatc cgccctgggc caccgcgcgg ccgggtggct cttgcgcggc
     3241 gcctgccggc atggcttgtg ccagtgtcat gcgtggactg gccgtggcgt gagggcgtta
     3301 ctgccagctg ccaatatcgg cattcttgcc ctcgccgccg gactggccgt cggcgccgaa
     3361 ggacatcaca tccacttcgc ccttgatgcc cgggttcagg tactggtagg ggtggcccca
     3421 ggggtcgttg ggcagctttt ccaggtaggg gcgccagttg ccgggcaccg gggccgtggc
     3481 gggcttggcg atcagtgcct gcaggccttg ttcggccgtg gggtagcgct ggttgtccag
     3541 gcggtagagc ttgagcgcct gtgagatgtt ggcgatgtcg gtgcgcccgg cggtcacgcg
     3601 cgcgtcgtcg gcgcgatcga gcacattggg cacgatgagc gcggccagca cgccgatgat
     3661 caccagcacc accatgagct cgatcagggt gaagcccgcg gccaggcggc ggcgggcatg
     3721 ttgtgcaaaa gaaggggggg aggagggcgt cacggcgcgg caaatccggt agagcgaaag
     3781 cgctcaatga taatgctggc atggcaacac tctcactcac ccgctgggcc ccgccccttg
     3841 ccactctggt gctgtgggca ctggccggcg ccagcgcggt ctattggggg ctgaggctgt
     3901 ccgagcccgc ggccggcccg gcgcccgtgg ccgccgcgcc cgagcccatc ctgcccgacg
     3961 cgcaggcgct ggcccggctg ctgggcgccc atgcccaggc ggccgccgcc acgccggccg
     4021 cggccagccg tttcacgctg ctgggtgtgc tggccggcac gcaaagtggt gacggcgcgg
     4081 cactgatcgc cgtggacggc aagcctcccc ggcattaccg cgtgggcgcc gccatcgagc
     4141 ccggcctggt actgcagtcg ctgggccggc gcgaggcgcg cctgggcgcc agtgtcgatg
     4201 gcgccaccac gctggccctg gagttgccgc gccagctggg cctgcagggg cagggcgcgc
     4261 cggcgactca gcccatgcgc cagcaggcgc cgcggatgca gcaggcgcca gcggcccagc
     4321 cgccccagca atacgaatag cccgcctgcc gtcaggctct caacctctca ggcccagccc
     4381 tgggttggca gggccacgct gacccaggcg ccaaaatcag gtgcctgcag cggccactca
     4441 tgcgcctgcg gcgcacgtcc tggcccatgc tgctgcagcc attgcacgca acgcgcaacc
     4501 ccggcatggc tgatccagac catgtccgcg ccctggtcat gggcgcgggc cgcggcatcg
     4561 cgcagggcca gggccacgcg ctgcagcatg gccgccagcg actcgccgcc gccgggagca
     4621 tggccggcca gattggcggc ccaggcgtcg atctcggcgc gggcgatggc gctccaggcc
     4681 tggccctccc aggcgccaaa gtcggtctcg gcgatgcgcg gctccgggtt tgaagccaaa
     4741 tcgggccgca gcgcccgcag ttcaagcgcc agcagctcgc atctttgtag tggcgaatgc
     4801 catacgccgg ccaccttggc cggcagggca tgtgccagtg ccgtggcggc gctgcggctg
     4861 gcgctggcgt cggcggccat gtccagccgg ccgtagcaca ggccgggcgc gaccagcggg
     4921 cgcgcatggc gcaccagcca cagccgcggg ccgttcatgc cgcgcgtggc gccagggcca
     4981 tggccgcgcc caggtaaaag ccaatctcgg ccacctgctg cgtggcgccc aggcagtcgc
     5041 cggtaaaccc ctgcaggcgg cgcgcaaaac gccacagcat ccaggcgccg cccagggcgc
     5101 tgcacagtac ggatactatt aaaacactag cgcctagcgc ttggtacata agcgctagcg
     5161 gcaaaaaaca ccacaagact gcggccgcca gggcgcgacc cgtgatctga tctgccagcg
     5221 gcttgctctt ggacgtggcc gtatccccca catggggcag ggcgcgcacc acgaaaagtg
     5281 gccacaggcg cgagagcaca tgcgcgccca gtagggccca cagcgccacc tccagcccca
     5341 ggctgcccag ctgcgccagc agcgccagct tggcgccaag ggccagcacc agggccatgg
     5401 cgccgaatgc gccaatgcgc gagtccttca tgatggtgag cgcacgctcg cgctcatagg
     5461 ccccgcccag gccgtcggcc acgtcggcca gaccgtcctc gtgaaagcca ccggtcacca
     5521 gcaccgtggc gatggtgctg cccacggctg ccaccagcgg cgcaaaaccc tggccgccca
     5581 gtgcccacca aagcgccgca tacacgccca tggccacggc tgccaccagc cagcccacac
     5641 cggggaaatg cgcgctggcc gcgcgcagca tggccgggct atagcccacc caggccgcca
     5701 gccggcccgt gaccggcagg cgggtgaaga actgcacggc cagcaggtag tggcgcaggg
     5761 cttgcatggg tgcttgttaa ctgatggctg ttggcgcttc catgccggcg agacgccggc
     5821 gctcccagta ggcgctgtca gcctccattg gccttcagga acagccggta cgccgggttc
     5881 tgcgtttcct cgacataggg gtagcccagc gcctgcaaga acgcgtcgaa cgcggcggca
     5941 tcctccgccg gcacctgcat gccgaccaga atgcggccat agtccgcgcc ctggttgcgg
     6001 tagtggaaca gcgagatgtt ccaggtcggc tgcatcaggc tcaagaactt gaacagcgcg
     6061 cccggccgct cggggaatgt gaagcgcatc aggcgctcgt cgcgcgccag ctgcgagcgc
     6121 ccgcccacca ggtggcgcag atgctccttg gccagctcgt cgtgcgtcag gtccagcgac
     6181 tcgaagcctt gtttggcgaa attcctggcg atcttctccg actcgccccg gccatgggtg
     6241 gacaggccca cgaacacatg ggccagccgc tcgtggctga tgcggtagtt gaactcggtc
     6301 acgttgcgcg gcccgccggg cagcgcgccc accacctcgc aaaagcgctt gaagctgccg
     6361 cgctcctcgg ggatggtgac ggcgaacagc gcctcgcgct cctcgcccac gtcggcgcgc
     6421 tcggccacga agcgcaggcg gtcgaagttc atgttcgcgc ccgagaggat ggtggcgtag
     6481 gtctcgccct tggtcttgtg cttcgccacg tattgcttga ttgccgccac gcccagggcg
     6541 ccggatggtt cgacgatgct gcgcgtgtcg gcaaagatgt ccttgatggc ggcgcagacg
     6601 gcgtcggtgt ccacgatcac gtagtcgtcc accaggtcat gcgccacgcg gaaggtctcc
     6661 tcgcccacca gcttcacggc cgtgccatcg gcaaacaggc ccacgtcggg cagctgcacg
     6721 cgcgcatgct gacgcgccga ttgcgccatg gcgtccgagt cgttcatctg cacgccgatg
     6781 accttggtct gcgggctcac cgccttcagg taattggcca cgccggcaat cagcccgccg
     6841 ccgccgatcg gcacgaacac ggcatccagc cggtggctgc ccagcgcctg cagctggcgc
     6901 agcagctcca tggcaatcgt gccctggccg gcgatcacgt ccgggtcgtc aaaggggtgg
     6961 acgaagctca ggccctgctc ctgctgcagg cgcagcgaat gctcgtaggc gtcggaatag
     7021 ctgtcgccca tcagcaccac ctcgccgccc agggtcttca ccgcgtccac cttcacctgc
     7081 ggcgtggtgg tcggcatgac gatcacggcg cgcgtgccca gcctggcggc gctcatggcc
     7141 acgccctgcg cgtggttgcc ggccgaggcg cagatcacgc ccttggccag ctgctcggcc
     7201 gacagctgcg ccatcttgtt gtaggcgccg cgcagcttga agctgaacac cggctgttga
     7261 tcctcgcgct tcaacagcac cttgttgtgc aggcgccgcg acagcgcccg cgccggctgc
     7321 aggtcggact ccacggctac gtcgtagacg cgcgccgtga gaattttctt caggtaatcg
     7381 aggggggtga ggggctgggt catgatgtca tccggcgcgg cgttcgtgcg cggccggccg
     7441 gggcgccatc ataggggcca gtccgcagcg gccgcgcggg gcaaaaaaaa gccccaagcg
     7501 gtaaagcctg gggctgaatc catcctttga ggagatggag gagacaatcg gtgcgaaccc
     7561 cgctctgtta acagtgcgta acgggtaacg catggctgca ttgtatgccc gaaggttttt
     7621 gcattgcagc aaaatcggta tttttttaag ggttcgccct aattgttgga gaaacgccac
     7681 atggaatgca ccgtcagctg gaccggcaat gccggcacaa gatccggcat gggatttatt
     7741 gccgaaaccg gcagcggcca tatcctggcc atggacggcg cgcccgacgc cgagcgcccc
     7801 gagagcggtg gccagaatct ggccccacgc cccatggagg ccgtgctggc cggcaccggc
     7861 ggttgcaccg cctacgacgt ggtgctgata ttgaagcgtg gccgccacga cgtgcgcggc
     7921 tgtagcgtgc gcttgacctc agagcgcgcc gccaccgagc ccaaggtctt cacccgcatc
     7981 cacatgcact tcaccgtcac cggtcgcggc attccggcca gcgcggtgga gcgtgccatc
     8041 gccatgagcc acgagaaata ctgctcggcc agcatcatgc tgggcaagac ggcgcagatc
     8101 accacgggct tcgagatcgt cgagggctga tgcgtcgatg ccagaggcct gcagtctggt
     8161 cgggcgcaga tccggccatg gcctggtggc gccaggcaaa tgttgcaggt ctgcaacgga
     8221 aaactgcgtt tacccccctt ttcggccgtt aatacgggaa tttctttgcg catcctggcg
     8281 ccctctgttg caataagacc aacttcccca ccaggaggtt ggcccgggga gccggcgggc
     8341 gggtgcgggt agcacccact aagaccctca cgatccggtg ccctctgttt aaccttggag
     8401 taactcgtaa tgaaaaaatc cctgattgcc ctggcagtgc tggccgcttc cggtgccgca
     8461 atggctcagt cctccgtgac cctgtacggc gtggctgacg ctggcgtgac ttacatcaac
     8521 ggcaaggaca actggtccgg cgtgagctcg ggcaaccaac tgaccagccg tatcggcttc
     8581 cgcggtgtgg aagacctggg tggcggtctg aaggccaact tccgcctgga agccggtctg
     8641 aatctggacg tgggtgacgg cgcttccggc tatgcaggtg ccaaggctgg tgaaggtctg
     8701 gcgttcaagc gtaactccac cgtgggcctg gaaggcggct tcggtgaagt gcgcctgggc
     8761 cgcgagctga ccgccgccta cgacgccgtg gcccgctacg acgtgttcgg cagcgtgggt
     8821 ctggcttact cccgcatgtg ggtggctggt gacacggctg agagcagcac caacgccacc
     8881 gccacggccc cgaacatccg cgtgagcaac gccgtcacct acgtgtcgcc caacttcgcc
     8941 ggcttcaagg ttggcgtgaa ctacggcttt ggcgaaatcg ccggcaagaa cagcgacgcc
     9001 cgctatctgg gcgctggcct aacctatgat aacggcccca tcagcgtggg tctggcagcc
     9061 gagcagctga agcagaatac cgttggtggc aattccagcg gttccgtcaa tggcgacatg
     9121 acctcctggg ctctgggtgc ctcgtacgac ttcggcgtgg ccaagctgct gggtgcctat
     9181 cgcacctcca agcatgagaa ctgggccaat aaccttattg gcaacgacaa caagcgcaat
     9241 ggctacctgc tggctgtgac cgctcctgtc ggccccggtc tggtgaaggc ttcgtacaac
     9301 cgctacgaaa acaagcttgg cggtaccacg accaaggctg accagttcgg cctgggctat
     9361 gtctacagcc tgtccaagcg cacttccgtg tacggcacct atgcctacct gaagaacaag
     9421 aacggcgcca gcctgtacac cctgggtgcc ggcggcctga agaacaatgg ctcgcagcaa
     9481 ggcgtgcaaa tgggcatcac ccacacgttc taatgccccg ctggcctgaa gccagctttg
     9541 cataaagaca aaaaaccgct gccttcgggc ggcggttttt tttcgtccgc agttaagcca
     9601 gtcgcctgcg taacaccaac ctaggggaaa cgcctgtggc atggatgcca tagtgtattg
     9661 gttcctgtga tggggcttcc tagaatgaaa ccttctgata ctttattgag agggagcgag
     9721 agaagccatg cgtctgttga ccaagaaaaa caccgtcctg gccgcgctgg ccctgtgtgc
     9781 caccggagcg gctatggccc aggcggccaa caccagcaag gtggagctgt ggggcatcgt
     9841 ggacgtggcc gtgcgccaca ccaataacga gggtgccgcc aagaccggcc tgacgaagat
     9901 gataggcggc ggcatgtcgc aaagccgctg gggcatcaac gtcgaggaag acctgggcgg
     9961 cggcagcaag gc
//
LOCUS       DAOMTQ010000111         9886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1362_length_9886_cov_24.351134, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000111 DAOMTQ010000000
VERSION     DAOMTQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1362_length_9886_cov_24.351134"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PK518_10960"
     CDS             complement(<1..1247)
                     /locus_tag="PK518_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49478.1"
                     /translation="MTDQQATAPDWSRFHGAMVSGTMQGEILSACERAWAWLELPAED
                     CARRIEDTIHSDVAARWQLQALPFVRHRLMHEAGIHPNDNAPRAALSDSVATGQASAA
                     ELELSRHLNATLAIDRLVTERFPDAVLPPASSDGQREALLGRTPALTEAMHACDVARA
                     TPQGLARLQAAIAVFDAAGSQAQGDIAREQAWWLGLAWWAAGRAALELGQQGAGRQAY
                     EHAVAHYTQAADTQAAEDCRERLRDLAARRTGDFDTAAGRELSALLLRRDPLGRARAL
                     CRLVRETLATGDRFEAARLAEQAALVLAEAGYPDPESDFDKAAQQWVDSACPTCTGSA
                     LIAQLCEIAQHWAAILGARASQRMQDDPAASEHAELVLRRISPWAGELLNQADAAEQA
                     TAQRFALWCPPQTQPLPERHPAAP"
     gene            complement(1275..2393)
                     /locus_tag="PK518_10965"
     CDS             complement(1275..2393)
                     /locus_tag="PK518_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49479.1"
                     /translation="MTQPFFTVEMLPARHGDALWIEYGDERRTRRILIDGGPLHAWPD
                     FAARIGKLPAGDQRIELLVITHVDTDHIEGIIRLLALPRAKWPLEPMDIWFNGYRQIE
                     AQKGLGGREGEFLSALIHRRAFDEWNRAFGRNAVVVPGKGPLPRIALPDKDGMVLTLL
                     SPDKAKLTAMGKRWRKDVAQWELDPGDLDAAWAQLVDEAKFHPGAELTLGPEDLTAQL
                     RAQLKGADASAANGSSIAFLAEYAGKSCLFLADAHMKTVCASIRRLLAPGQDALRVDA
                     VKLAHHGSRNNLTPEFMQLVDAEHFLFSSNGDKFKHPDRQAVQAVIHGARRKPTLWFN
                     YRSAFNERWEAGSQAANAAYATRYPAPGAAGITVPLLA"
     gene            complement(2396..5311)
                     /locus_tag="PK518_10970"
     CDS             complement(2396..5311)
                     /locus_tag="PK518_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49480.1"
                     /translation="MKFDFWIRWGVNLSDADFAAAWLALGIAAGAARKPPAAKGDLAR
                     ARAAAATPDDGLAIARDALTGALSVAAEQDQGVNWRFPDPDDCVDADGRLPLSRIAPA
                     AWAQAWGAAAGQAPAPRLRVTNAPEDEPYLPWLRLLSEARPKVAAVSMAWQAPNPQLV
                     LAWPLRLGAVSAEGEAVIAAAREQWPSDRLTRRVGIDRDNANCDVLVHSGTLATLLSA
                     LAAQPFHCKANLVLVASASDAGGAAVLKQIRAIAQKTNASGVLLWTQPADARASGLAL
                     NAFINEFSHDLPLDQALHAAAQREPVGEASGWLTDAVAGLHLHALADTLVARAQALPA
                     GTAIKVKDLSAHWVKRIAPVTTRAGGFAGASKAMPEGYEDVDTVQLDTSQLDYLGESH
                     GASELATLGDALANARTTTKAETHRAARYLQQQSFVRRRGRYAAAAGGFVAGAPALVR
                     VRIGPPQGKWQSTPAEFPAEALPPGYQQWRLTVWLTEARQLDEPLRKTITLPIDGAST
                     ECEFRFTPRSAGPFDGRISVLHRGRVLQTAALRGGVIADGATADAKAMPVLADIVPVR
                     HRLGDLEGRRQFDLAFVTNHTSAGEPRAVALSDDHAWISDVTKSLAVTQDINTALSKV
                     AKSVADYTDGLSGEKGRALFVDLAKKGAWLQLYLVSNQIQRPGNRPDVAEQEYIQIVS
                     TRSDAIVPFEFIYDHEVPDDDAAICPQWRDALEHGNCAQGCNGGDPRRVCPLGFWGLR
                     KVIERHQVSPELAAPGRELFLQSEPGRDTTDLPLGGTALFASSQKVPNAAQNTVTAAL
                     TQKLGLAPMQATNWSDWATMVGQARPHFLLALPHTDGTGANVTLEIGGTTIGTIQVKP
                     AHVHPAKDDARPLVALLGCDTAGTANEYAEHVAVFRDRGAAVVIGTIATVFGEHAARV
                     GVLLAQELLPDGAPPQRLGEAMRALKRRALLEDLLMPLCVVAYGDADWRLVREVAGG"
     gene            complement(5357..6370)
                     /locus_tag="PK518_10975"
     CDS             complement(5357..6370)
                     /locus_tag="PK518_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRM49481.1"
                     /translation="MALTIGGTARRIGLALSGGGFRAAAFHLGVMRRLAALGILEHVD
                     LFTCVSGGSIAGATVIKHWADPARLDILDRYLRTRSIAVSSVIGGVLDPFEDRLDKLA
                     ASYDRDLFDGATLDGLGGGPRLYVNATNLATGNLFFFVTGGGKPAEMGEHELGVVAAP
                     GFPVCRAVAASSAFPPVFPPLRLDASSYAHASSVEYVTLTDGGVYDNMGINPLVRARN
                     ALDYVIVSDGGKPFALDERPTESGAIVLKAALDIMMEQIRGLEFDRLQHRHAALKGPQ
                     PLWFSIDSREGEAQAGDAAFASSIKTNLTRLGREEMDVLTRHGGALVEARIRRYAPEL
                     IGA"
     gene            6534..7115
                     /locus_tag="PK518_10980"
     CDS             6534..7115
                     /locus_tag="PK518_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49482.1"
                     /translation="MKFFWPTGVVCAVLLAGCAGQQTLVPQVRQAVADGGAKTVTVAP
                     EPLACTRTQCPVLAGAWSSAKTGLAVLTIGLPHQSAEVTGADFHFGSSQVVRVRSRSQ
                     APAPTLGFPATAFDVPMTLVGQIAYAPRSWMRVYTADGRSVDETINSGEQRSRVVDAM
                     HHFFTTIEAAGGNTGTEPANRGGLFDRLGFEKK"
     gene            complement(7162..8001)
                     /locus_tag="PK518_10985"
     CDS             complement(7162..8001)
                     /locus_tag="PK518_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRM49483.1"
                     /translation="MDSVLQLGPLVLPWALIILVVGWQLGHWVHERLARRSGLTPGPH
                     AWRLTLAALLAARLGFVLQYRAEYGAAPWSVLDIRDGGWQPWAGLAAALLYVGLLWLR
                     RSPWRQPALAGVAVFATIWLAGLALLQAMAPQRPTELPDWRGVALDARGVALPELRGQ
                     SVVINLWASWCPPCRREMPVLLQASRAHPEVRFLWINQGEPPETALRYASQQGLPAAD
                     VLLDTASQLGRDLDSKALPTTLFYDAQGRLQAVRAGELSAATLAQHLGRITASGTARQ
                     ASK"
     gene            complement(7994..8746)
                     /locus_tag="PK518_10990"
     CDS             complement(7994..8746)
                     /locus_tag="PK518_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM49484.1"
                     /translation="MPKPSRMHILIVEDNTLVASGIRVGLELHGFASDTAASVAQAEA
                     HLAARSYDACVLDLGLPDGDGMQLLQLARVESASSAETPETQGAAIAQAVRLALQQSQ
                     QCAQTEQGAHAPVQLVQTPADMGAWQHIAIALPPALLTCAVTNLLDNALRHHRGSTAI
                     TIALRLCEGASVDISVRDHGPGLTEQECTQALQRFWRKGGSGPGSGLGLTIARRIAES
                     AGGSLHLAPAQPGLLARLQLPTLAPATEVGHG"
     gene            complement(9082..9477)
                     /locus_tag="PK518_10995"
     CDS             complement(9082..9477)
                     /locus_tag="PK518_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRM49485.1"
                     /translation="MRFPVVLHSDDGVRYGVTVPDLPGCFSAGDTFDDALASVVEAID
                     LHLEGVTEDGGDIPVPRAIGEHRTNPDYADGVWAAVDVDVSRFDGRAEKINITVPRNL
                     LAKIDSYAKSHGATRSGFLADAARLAMRD"
     gene            complement(9484..9666)
                     /locus_tag="PK518_11000"
     CDS             complement(9484..9666)
                     /locus_tag="PK518_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRM49486.1"
                     /translation="MNSKQVIKRLEAEGWFLARVKGSHHQFKHPSKPGLVTVKHPDSD
                     IPAGTLRSIEKQAGWR"
BASE COUNT         1524 a         3318 c         3420 g         1624 t
ORIGIN      
        1 ggggcggccg ggtgccgttc gggcaaaggc tgggtttgtg gtgggcacca cagggcaaag
       61 cgctgcgcgg tggcttgttc ggccgcgtcg gcctggttga gcagttcgcc cgcccagggg
      121 gatatgcggc gcagcacgag ttcggcatgt tcgctggcgg cggggtcgtc ctgcatgcgc
      181 tggctggccc gggccccgag gatggcggcc cagtgctggg cgatctcgca cagctgggcg
      241 atgagagcgc tgccggtgca ggtggggcaa gcgctgtcca cccattgctg cgcagccttg
      301 tcgaaatcgc tttccggatc cgggtaaccc gcctcggcca gcaccagcgc ggcctgctcg
      361 gccaggcgag cggcctcgaa gcggtcgccc gtggcaagcg tctcgcgtac cagccggcac
      421 agcgcccggg cgcgcccaag tgggtcgcgg cgcaggagca gggcggacag ctcgcgcccg
      481 gccgccgtgt cgaagtcgcc cgtgcgccgc gcggccaggt cgcgcaggcg ttctcggcag
      541 tcttcggccg cctgtgtgtc ggcggcctgg gtgtaatgcg ccacggcgtg ctcgtaggcc
      601 tgacggcctg cgccctgctg gccaagctcc agcgccgcgc gcccggcggc ccaccaggcg
      661 aggcccagcc accaggcctg ttcgcgcgcg atgtcgccct gcgcctggga gcctgcggcg
      721 tcaaagacgg caatggctgc ctgcagccgc gccagccctt gcggcgtggc gcgggcgacg
      781 tcacaggcgt gcatggcctc ggtcaaggcg ggtgtgcggc cgagcagcgc ctcgcgctgc
      841 ccgtccgacg acgcgggcgg cagtaccgca tcaggaaagc gctcggtcac cagccggtcg
      901 atggccagcg tggcattcag gtggcgcgac agttcaagct cggcagcgct ggcctgcccc
      961 gttgccacgg agtcggacag ggcggcacgc ggcgcgttgt cgttggggtg aatgcctgcc
     1021 tcatgcatca gccggtgccg cacgaagggc agggcctgca gctgccagcg ggcggcaaca
     1081 tcgctgtgga tggtgtcttc gatccggcgc gcgcagtctt ctgcaggcag ctccagccag
     1141 gcccacgcgc gttcacaggc cgagaggatc tcgccctgca tcgtgcccga gaccatggct
     1201 ccgtgaaatc ggctccagtc cggcgccgtt gcttgctggt ctgtcatgcg tatctcgttt
     1261 ggtggagggc agcttcaggc cagcagtggc acggtgatgc cggccgcgcc cggcgcggga
     1321 tagcgcgtgg cgtaggccgc gttggcagct tgcgagccgg cttcccagcg ctcgttgaag
     1381 gcgctgcggt agttgaacca cagcgtgggc ttgcggcgcg cgccgtggat cacggcctgg
     1441 accgcctgcc tgtccggatg cttgaacttg tcgccgttgc tgctgaacag gaaatgctcg
     1501 gcgtccacca gctgcatgaa ttccggcgtc aggttgttgc ggctgccgtg gtgggccagc
     1561 ttcacggcgt ccacgcgcag cgcatcctgg cccggcgcaa gcagacggcg gatggaggcg
     1621 cacacggtct tcatgtgcgc gtcggccagg aacaggcagg acttgcccgc gtattcggcc
     1681 aggaaggcaa tgctgctgcc gttggctgcc gatgcgtctg cacccttgag ctgggcgcgc
     1741 aactgggccg tcaaatcctc aggccccagg gtcagctcgg cgccggggtg aaatttggct
     1801 tcatcgacca attgcgccca ggcggcgtcc agatcccccg ggtccagctc ccactgcgcc
     1861 acatccttgc gccagcgctt tcccatggcg gtcagcttgg ccttgtcggg cgacagcagc
     1921 gtgagcacca tgccgtcctt gtccggcagg gcaatgcgtg gcagtggccc cttgcccggc
     1981 acgaccacgg cattgcgacc aaaggcccgg ttccactcgt caaaggcacg ccggtggatg
     2041 agggcgctga ggaactcacc ctcgcgcccg cccaaaccct tttgcgcctc gatctgccgg
     2101 tagccgttga accagatgtc catgggctcc agcggccact tggcacgcgg cagtgccagc
     2161 aggcggatga tgccctcgat atggtcagtg tccacatgcg tgatcaccag cagctcgata
     2221 cgctggtcac ccgcggggag cttgccgatg cgcgccgcaa agtccggcca ggcgtgcagc
     2281 gggccgccgt cgatcaggat gcggcgcgtg cggcgctcgt cgccatactc gatccacagc
     2341 gcgtcgccgt gcctggctgg cagcatctct accgtgaaga atggctgggt catggtcatc
     2401 ctcctgcaac ttcacgcacc agccgccagt cggcatcgcc ataggcgacc acgcacagcg
     2461 gcatgagcag gtcttcaagc agtgcccggc gcttgagcgc gcgcatggcc tcgcccaggc
     2521 gttgcggcgg cgcgccgtcg ggcagcagct cctgcgccaa cagcactccc acgcgggccg
     2581 catgctcgcc aaacacggtg gcgatggtgc ctatcactac ggcggcgccg cgatcgcgga
     2641 acaccgccac atgctcggcg tattcgttgg cggtgccggc ggtgtcgcag cctagcaggg
     2701 cgaccagcgg gcgtgcgtcg tccttggccg ggtgcacatg cgccggcttg acctggatgg
     2761 tgcctatggt ggtgccgcct atctccagcg tgacgttggc gccggtgccg tcggtgtgcg
     2821 gcagtgccag caggaaatgc ggcctggcct ggccgaccat ggtggcccag tcggaccagt
     2881 tggttgcctg catgggcgcc aggcccagct tttgtgtgag tgccgccgtc acggtgttct
     2941 gcgccgcatt gggtaccttc tggctgctgg caaacaaggc cgtgccgccc aggggtaggt
     3001 ccgtcgtatc ccgccccggc tccgactgca ggaaaagctc ccgtcccggg gccgcgagtt
     3061 cggggcttac ctggtggcgt tctatgacct tgcgcaggcc ccagaacccc agggggcaga
     3121 cccgcctggg gtcgccacca ttgcatcctt gcgcacagtt gccgtgctcc agcgcatcgc
     3181 gccactgcgg gcagatggct gcatcgtcgt ccggcacctc atggtcgtag atgaactcga
     3241 agggcacgat ggcatcgctg cgcgtgctga cgatctggat atattcctgc tcggccacat
     3301 cgggccggtt gccggggcgc tgaatctggt tgcttaccag gtacagctgc agccaggcgc
     3361 ctttcttggc cagatccacg aacagtgcgc gccccttctc gccagagagc ccgtcggtgt
     3421 agtcggccac cgacttggcc accttggaga gcgcggtgtt gatgtcctgc gtgaccgcca
     3481 ggctcttggt gacatcggaa atccacgcgt gatcgtccga aagggcgacc gcacgcggct
     3541 cgcccgcact cgtgtggttg gtgacaaacg ccaggtcgaa ctggcgccgg ccctccaggt
     3601 cgcccaggcg atggcgcacc ggcacgatgt cagcgagcac cggcatggcc ttggcatccg
     3661 cggttgcgcc gtcggcgatg acgccgccac gcagcgcggc ggtctgcagc acgcgcccgc
     3721 ggtgcagcac gctgatgcgg ccgtcgaagg ggccggcgct gcggggagtg aagcggaact
     3781 cacattcggt gctggcacca tcgattggca gggtgatggt cttgcgcaag ggttcatcca
     3841 gttgcctggc ttcggtcagc cagacggtga gccgccattg ctggtagccc ggcggcagcg
     3901 cctctgccgg aaactccgcc ggggtggact gccatttgcc ctggggcggg cctatgcgca
     3961 cgcgcaccag cgcgggtgcg ccggcgacaa agccgccggc cgccgcggca tatcggccgc
     4021 gccggcgcac aaaggactgc tgctgcaggt agcgcgcggc gcggtgcgtc tcggccttgg
     4081 tcgtggtgcg tgcattcgcc agcgcgtcgc ccagggtcgc aagctcggag gcgccatggg
     4141 attcgccaag gtagtccagc tgactggtgt ctagttgcac cgtgtccacg tcctcatagc
     4201 cctccggcat ggccttggag gcaccggcaa aaccgcccgc gcgcgttgtg acaggcgcaa
     4261 tgcgttttac ccaatgcgcg ctcaaatcct taaccttgat ggcggtgccg gcgggcagcg
     4321 cctgcgcacg cgccaccagc gtgtcggcca gcgcatgcag atgcaggccg gcaacagcat
     4381 cggtgagcca gccgctcgcc tcgcctacgg gttcgcgctg ggcggcggca tgcagagcct
     4441 gatccagcgg caggtcgtgc gagaactcat tgatgaaggc attcagcgcc aacccgctgg
     4501 cgcgggcatc ggcgggctgc gtccacagca ggacgccgct ggcattggtc ttctgcgcaa
     4561 tggcgcggat ttgttttagc actgctgcgc cacccgcgtc gctggcggag gcgaccagta
     4621 ccaggttggc cttgcaatga aacggctgcg ccgcaagggc ggagagcagc gtagccaagg
     4681 tgccgctgtg taccagcaca tcgcagttgg cgttgtcgcg gtctatgccg acgcgccggg
     4741 tcagtcgatc gctgggccat tgctcccgcg cggcggcgat tacggcctcg ccctcggcag
     4801 acacggcgcc caggcgcagc ggccaggcca gcaccagctg ggggttgggt gcctgccagg
     4861 ccatggagac ggcggcaacc ttggggcggg cctcggacag caggcgcagc cagggcaggt
     4921 atggctcgtc ttccggcgcg ttggtcacgc gcaggcgggg tgctggcgcc tggcctgctg
     4981 ctgcacccca ggcctgggcc caggccgccg gggcgatgcg gctcaggggc aggcggccat
     5041 cggcatccac gcagtcgtca ggatcaggga agcgccagtt cacaccttgg tcctgttcgg
     5101 cggcaaccga tagcgcaccg gtcagcgcgt cgcgcgcaat cgccagccca tcgtccggcg
     5161 tggcggcggc ggcccgcgcc cgcgccaagt cacccttcgc ggcaggcggc ttgcgggcgg
     5221 cgccagcggc aatgcccagt gccagccagg ccgcggcaaa gtcggcgtcg gacaggttca
     5281 cgccccagcg gatccagaaa tcgaacttca tggtcggtct ccagtggtgg atcgggtgcc
     5341 ccgcgtgccg ggggagtcat gcgcctatca gctcgggcgc gtagcggcgg atgcgtgcct
     5401 ccaccagcgc gccgccatgg cgcgtcagta cgtccatctc ctcgcggccc agccgcgtca
     5461 ggttggtctt gatggaggag gcgaatgccg catcgccggc ctgcgcctcg ccctcgcggc
     5521 tgtctatcga aaaccatagc ggctgcgggc ctttcagcgc cgcgtgccgg tgttgcagcc
     5581 ggtcaaactc caggccacgg atctgctcca tcatgatgtc cagcgccgcc ttcaagacga
     5641 tggcgccgga ctcggtgggg cgctcgtcca gggcaaaggg cttgccgccg tcgctgacga
     5701 tgacgtagtc gagcgcattg cgtgcgcgca ccagggggtt gatgcccatg ttgtcgtaca
     5761 cgccgccgtc ggtgagcgtg acgtactcca cgctgctggc gtgcgcatag ctgctggcat
     5821 ccaggcgcag cggcggaaac accggcggaa acgccgacga cgccgccacc gcccggcaca
     5881 ccggaaagcc gggcgcggcc accacgccca gttcatgctc gcccatctcg gccggtttgc
     5941 cgccgccggt gacaaagaag aacaggttgc cggtggccag gttggtggcg ttgacgtaca
     6001 ggcgcggccc gccgcccagt ccatccaggg ttgcgccgtc aaagaggtcg cggtcgtagc
     6061 ttgcggccag cttgtccagg cgatcctcga atgggtcgag cacgccgccg atcaccgacg
     6121 acacggcgat agatcgggtg cgcaggtagc gatcgaggat gtccagccgg gccgggtcgg
     6181 cccagtgctt gatcacggtg gcgccggcaa tgctgccgcc ggagacgcag gtgaacaggt
     6241 cgacatgctc cagaatgccc agcgccgcca gcctgcgcat gacccccagg tggaacgccg
     6301 cggcgcgaaa accgccgccc gacaatgcca ggccaatgcg cctggcggta ccgccaatcg
     6361 tcaaagccat gccagcctcc ttggccgcgc cgggcagccg gttggttgcg ccgcgacgct
     6421 tgaatcatca tagttctgtc gcctgcgtga cgccagacgc acgaccgcgc gcgatgggct
     6481 gggccatgcc tttattgggt atcgtcgggc accaacgata aggagtctcg aacatgaaat
     6541 ttttctggcc tacaggggtg gtttgcgcgg ttctcctggc cggttgtgcc ggccagcaaa
     6601 ccctggtgcc ccaggtacgg caagccgtgg ccgatggcgg cgccaagacc gtgaccgtgg
     6661 cgcccgaacc cctggcttgc acacgcacgc aatgcccggt gctggccggc gcctggagca
     6721 gtgccaagac tggcctggcg gtgctcacga tcggcctgcc gcaccagtcg gccgaggtca
     6781 cgggggcgga cttccacttt ggcagcagcc aggtggtgcg cgtgcgttcg cgctcgcagg
     6841 cgccagcgcc gacgctgggc tttccggcca cggcttttga cgtgcccatg accctggtgg
     6901 gccagatcgc ctacgcgcca cgcagctgga tgcgggtgta cacggccgat ggccgcagcg
     6961 tggacgagac catcaacagc ggcgagcagc gcagccgcgt ggtggacgcc atgcaccatt
     7021 tcttcaccac catcgaggcc gccggcggca acacgggcac ggagccggcc aaccggggcg
     7081 ggttgtttga tcgacttggg tttgagaaga aataggctac aagcgccatt aataaagcgc
     7141 aaacagctat taatttaata gttatttgct ggcctgccgc gccgtcccag aggcggtgat
     7201 gcgccccaga tgctgcgcca gcgtggcggc cgacagctcg cccgcgcgca cggcctgcag
     7261 ccggccctgg gcgtcgtaga acagcgtggt cggcagggcc ttgctgtcca gatcgcggcc
     7321 cagctgcgat gccgtgtcca gcagcacatc ggccgcgggc aggccctgct ggctggcgta
     7381 gcgcagcgcc gtctcgggcg gctcgccctg gttgatccag agaaagcgca cctcggggtg
     7441 cgcacggctg gcctgcagca gcaccggcat ctcgcgccgg cagggtgggc accagctggc
     7501 ccacaggttg atcaccacgc tctggccgcg cagctcgggc agggccacgc cgcgtgcgtc
     7561 cagcgccacg ccgcgccagt cgggcagctc ggtcgggcgc tggggtgcca tggcctgcag
     7621 cagggccagg cccgccagcc agatggtggc aaacaccgcc acgccggcca gtgccggctg
     7681 gcgccagggg ctgcgccgca gccacagcag gcccacgtag agcagggcgg ccgccaggcc
     7741 ggcccagggc tgccagccgc cgtcgcgtat gtccagcacc gaccagggcg cggcgccata
     7801 ttcagcccgg tactgcagca caaagcccag gcgcgcggcc agcagggctg ccagcgtcag
     7861 gcgccaggca tgcgggccgg gcgtcagacc gctgcgcctg gccaaccgtt cgtgtaccca
     7921 gtggcccaac tgccagccaa ccaccaggat gatcagcgcc cagggcagca ccagggggcc
     7981 gagctgcaac acgctatcca tggcccacct cggtggcggg cgccagcgtg ggcagctgca
     8041 gccgggccag caggccgggc tgggccggcg ccaggtgcag gctgccgccg gcgctttcgg
     8101 caatgcggcg tgcaatggtc aggcccaggc cgctgcccgg gccgctgccg cccttgcgcc
     8161 aaaagcgctg cagcgcctgc gtgcattcct gctcggtcag gcccgggcca tggtcgcgta
     8221 cgctgatgtc cacactggcg ccctcgcaca gccgcagcgc gatggttatc gccgtgctgc
     8281 cccggtggtg gcgcagggcg ttgtccagca ggttggtgac ggcgcaggtc agcagcgccg
     8341 ggggcagggc gatggcaatg tgctgccagg cgcccatgtc ggcgggcgtt tgcaccagct
     8401 gcaccggggc gtgcgccccc tgttcggtct gggcgcactg ttgcgactgc tgcagcgcca
     8461 ggcgcacggc ctgggcgatg gccgcgccct gggtctccgg cgtctcggcg ctgctggcgc
     8521 tctccacgcg cgccaactgc agcagctgca tgccgtcgcc gtccggcagg cccaggtcaa
     8581 gcacgcaggc gtcgtagctg cgcgccgcca gatgggcctc ggcctgggcc acgctggccg
     8641 ccgtgtcgct ggcaaaacca tgcagctcca gcccgacccg gataccgctg gccaccaggg
     8701 tgttgtcttc gacgatcagg atgtgcatgc gcgatggctt gggcaaagcc ggtattctag
     8761 aaacggcacg tcatactgta ttgtggtgcg gcggggggca ggaagtgccg gcagctggaa
     8821 gaagggggcg ggcattgacg ggcggtatgc accagatacc agccgtcggc cttcaggccc
     8881 tgggtgattt ctgcgtcagg cgcggcggtc tcaactccga agtgcaacac ccctggctgc
     8941 gctgattcag gcagattgag actacatgag ctaccttgat ggctctggcg cagccggcat
     9001 tgtggccgat tgaaatgagc ggctgcgcct gcaacgactt gttggccccc actctggaac
     9061 cccgatgaat cacgctgcca atcagtcccg catcgccagc cgcgctgcat cggccagaaa
     9121 gccgctgcgc gttgcaccgt ggctcttggc gtaggagtcg atcttggcca gcaggttgcg
     9181 cggcacggtg atattgattt tctcggcccg cccatcgaag cgcgacacat ctacatccac
     9241 cgccgcccac acgccatcgg catagtcggg attggtgcgg tgttcgccaa tggcgcgcgg
     9301 cacgggaata tcgccgccgt cttcggtgac accctccaga tgcaggtcga tggcttccac
     9361 caccgaggct aacgcatcat cgaacgtatc gcctgccgaa aagcaaccgg gcaaatcggg
     9421 cacggtgacg ccgtagcgga cgccatcgtc ggaatgaaga acaaccggaa agcgcatggc
     9481 gtattacctc caacccgcct gtttctcgat gctcctaagc gtaccggcgg gaatgtcgct
     9541 gtcggggtgc ttgaccgtca ccaagccggg cttggacgga tgcttgaact ggtgatgcga
     9601 gcctttcacg cgcgccagaa accagccttc tgcttccaat cgcttgatga cttgcttgct
     9661 gttcatggtg gttgctatac ccaccatgat aaatctgtca acaccataac caccacctat
     9721 ctacgcttga ccccatcgag caaccggtaa ttacggctaa cgccggaaat cagcggccat
     9781 ggtgacggga tttttcagct tggaggcata tgggtttttg cgagatttca acttggcgag
     9841 gtcgcattca gtcttcatgt ctagccacct tgtaaaacgt gctttc
//
LOCUS       DAOMTQ010000112         9859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1369_length_9859_cov_26.094961, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000112 DAOMTQ010000000
VERSION     DAOMTQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1369_length_9859_cov_26.094961"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PK518_11005"
     CDS             <1..971
                     /locus_tag="PK518_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM49487.1"
                     /translation="LTALGLADWRSNAAYSVPIGLTSWLVIDLGRLAWTSEPDIPWPA
                     GWRGPVLVAAGIGAGWLLGSAVGDWWLFGRLGWSSGGKWLMSLLITVVASVLASVFFY
                     QRSQARYLQAHMARAQRDAAEARLKLLEVQLEPHMLFNTLANLRALMATDVPRAQAML
                     DHLIAYLRATLGASRATLHPLEDEFARLADYLALMAMRMGPRLVYALDLPEPLRGLPV
                     PPLLLQPLVENAIRHGLEPQVAGGRIDVQARLLQAPDQAARLRIEVRDTGPGPGGGEP
                     GTAFGLQQVRERLATLYGTSATLELIAISEGGTSAVVTFPLKPEQP"
     gene            968..1768
                     /locus_tag="PK518_11010"
     CDS             968..1768
                     /locus_tag="PK518_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM49488.1"
                     /translation="MTAPRALIAEDEPLLAAALEQELARAWPELAIAVRVGDGLSAVR
                     QALALLPDILFLDIRMPGCSGLEAALELADAWPQGQPLPALVFVTAYDQYAVQAFEAQ
                     AVDYLLKPVQPERLRKSVDRLRQALAQRDTAWAQEQTLAQLRQLAQAPGLDRPPTAAP
                     LTLLQASRGNQIHMVALAEVLYFEAADKYLRVLTARQEYLIRTPLRELVAQLDPAVFW
                     QVHRGTLVRATAIASAERDEAGRLYLHLRDRPERLAVSRLYAPLFKAM"
     gene            1852..3072
                     /locus_tag="PK518_11015"
     CDS             1852..3072
                     /locus_tag="PK518_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49489.1"
                     /translation="MPARTALLMLVALTSGFALSSAFRTVAAILAAPLQADFALSPQA
                     LGVFAATFHFTFGAMQLFMGIGIDLYGVRRTILAAFPLCIAGAVVSALAPGYGALLLG
                     QALIGVGCAPAFLVCTVFIARHFAPQRFAAISGVAMALGTLGMLFTGTPLAWLVQQWS
                     WRAGFWVLALLALLAWLWIWRTVHEPTPDGTPEGPRESVREAVLRFASLFTLPHTWGI
                     VVLGAVTYAALISLRGLWLGPMLIERHGFSLVQSGNVALALSVVALAGPMLFGRIDPG
                     PATRRRWIVACTLLMALLFTAMAVLHAAWLDMACMLLIGLLSGYLILQYADVRAAYPA
                     ALTGRAMAVFTMAMFLGVAAMQWFTGLVASLAKAQAMADPFVAVMASIAALLALAALG
                     FRLLPQPPREGGVG"
     gene            3196..3504
                     /locus_tag="PK518_11020"
     CDS             3196..3504
                     /locus_tag="PK518_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I cytochrome c"
                     /protein_id="HRM49490.1"
                     /translation="MKNSLTAGLALCLLAATGLAHASSDPLQVRSWAAACANCHGTNG
                     QAQPGNEPLAGGNKDEMLKKLMDFKSGAKPATIMHQLSKGYTDEQLAAIAGWFAAQKK
                     "
     gene            3519..4802
                     /locus_tag="PK518_11025"
     CDS             3519..4802
                     /locus_tag="PK518_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM49491.1"
                     /translation="MDRRQFIRAGSAASAATALGLTAGCASTGSAAGAKVVVVGGGYG
                     GATAAKYIRMWSEGRIEVTLVEPNDSFVSCPLSNLVLGGSKQMADITTPYDNLTRRHG
                     VKIARDRATAIDADKRQVRLAGGQTLAYDRLVLSPGVDFMWDALPGMKKAGAQDRVLH
                     AWKAGPQTLALRKQLEAMPDGGVYAISIPLAPYRCPPGPYERACQVAHYFSQAKPKSK
                     VLILDGNDDVTSKGPLFKKAWADRYKGIIEYRPKHSTVDVDTATNTLKFDFNDDVKAQ
                     VLNVIPPQRAGDIAHNAGLTTANRRWCEVDWLTMESTAAKNIHILGDALQIAPQMPKS
                     GHMANQHGKVAAAAIVALLSGRAPNALPIYNNTCYSFVSDTDVVHVASVHRYDAEKKT
                     MLTVPGSGGVSAAASELEGRYGLAWARNIWADTLA"
     gene            4875..5489
                     /locus_tag="PK518_11030"
     CDS             4875..5489
                     /locus_tag="PK518_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM49492.1"
                     /translation="MRTQPIRHGLLALAFLSLATSAAAQGSDDEARAKKIIGGSCFLC
                     HGAEGESASAIYPRLAGQNAEYIAKQLENFKNGQRKSTAMAPMVASLAPEDMVALGKF
                     FASRPAQQKKARDQDLAAVGRYIYHHGNRFSGIPACATCHGPDAAGSATLPRLAGQHV
                     AYTEDQLKLFNKRERTNDNAVMHAVVEKMTALEMAAVAEYLSGK"
     assembly_gap    5496..5595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5596..6698)
                     /locus_tag="PK518_11035"
     CDS             complement(<5596..6698)
                     /locus_tag="PK518_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRM49493.1"
                     /translation="MTIDLATLVERHGTAGVLSVGGAVIGLLFGFFAQRSRFCLRAAV
                     IEFWRGKFAEKLSVWLLAFATALIAVQGLILAGWLDVSGVRQLANRGSLSGALVGGLL
                     FGVGMVMTRGCASRLLILSANGNLRALLSGLVFAVTAQATLAGSLAPLRSEIAGWWTV
                     EGGSARNLLALVGAGHWDGLVLGALWLAVALYFSWRSGNRAWMWLGGLCTGLAVALAW
                     WFNHAVAQASFDVVPVGGLTFSGPSAEWLMRVLANPGTTFGFDAGMLPAVFVGSLLGA
                     LVGREFKIEGFKDGYSMARYIAGAMAMGFGSMLAGGCAVGAGMTGGAIFSLTAWLALA
                     GMWLGAGFMDRWLAEPIHSLQPVTQQAPIVEQLA"
     gene            6743..>7419
                     /locus_tag="PK518_11040"
     CDS             6743..>7419
                     /locus_tag="PK518_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM49494.1"
                     /translation="MQPTPSRRQILQGCACLGLGGMLGGRWAGYAQAADKPPAAPAMQ
                     AHEAAPGCWYVEGLSALGSPANQNFISNAGFIVTSTSVVVVDALGSPALAERLLAEIA
                     RVTDKPVSHVIVTHYHADHVYGLQVFADRGIPILAHQAGREYLHADTARLRLQASREE
                     LAPWINDKTRLVPATQWVDGRQELTVGDTQLVLQPVGPAHTPEDLAVYLPQRKVLYAG
                     DLVFRSR"
     assembly_gap    7420..7519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7520..7790
                     /locus_tag="PK518_11045"
     CDS             <7520..7790
                     /locus_tag="PK518_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49495.1"
                     /translation="NYLAPMHAREGLAVVQVDMRGTQVTRNLQGQPSTHDALVRAWQV
                     KAAPTVLFLGPGGQEVADRLVGGSPDFYSAYLDGRLERARQALGK"
     gene            7871..8206
                     /locus_tag="PK518_11050"
     CDS             7871..8206
                     /locus_tag="PK518_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49496.1"
                     /translation="MPPLTSQWLLLLALGMGSAASAQEPALFKNADLKLGQRLIAENK
                     CAECHARKWADDGNAIYRPKGRINTPALLQGMVERCSSELSLNLFPEEITAISAALNH
                     AHYKFRDAP"
     gene            complement(8249..8677)
                     /locus_tag="PK518_11055"
     CDS             complement(8249..8677)
                     /locus_tag="PK518_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRM49497.1"
                     /translation="MNALVSRSGLFDDFFKDFAPAFYVRPLHGDALPEPSQIKIDVKE
                     DDAAYTVHAEVPGVPKEDINVSIDGNVVSLRAEVRQHDQKKDGEKLLRSERYYGSVAR
                     SFQLPVEVDAAQARARYDNGVLTLTLPKKQGNKTQRLAIE"
     gene            8848..>9859
                     /locus_tag="PK518_11060"
     CDS             8848..>9859
                     /locus_tag="PK518_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49498.1"
                     /translation="MLAALLSSFSWRALRHHPWRSAAAVAAVMLGVALGFAVHVINAS
                     ALGEFSQAMRSVGGQPDLELRATRGHLPESLYGLVAAQPQVALASPWLELPVRASSAA
                     APDGVPLRLLGADALLLPAMAPALAPRPHAGADRLAMLAPATVFLNAAAQQALGLASI
                     DADSPALTLHAGLQQHLVRVAGVVAAGGAPLTVMDIGAAQDLFGRQGQLTRIDLRLRP
                     GHTRAQLQEALAALPGWPAGVQFAEPQDALQRVDSLSRAYRVNLTVLALVALFTGAFL
                     VYSVLALSVAQRAPQLALLAVLGATPRERLALVLLESAALGVVGSLAGIALGTLLAAL
                     ALA"
BASE COUNT         1521 a         3465 c         3222 g         1451 t
ORIGIN      
        1 cgctgacggc gctgggcctg gcggactggc gcagcaatgc cgcctactcc gtccccatag
       61 gactgaccag ctggctggtg atcgacctgg gccgcctggc ctggacgagc gagccggaca
      121 ttccctggcc cgccggctgg cgcggcccgg tgctggtggc cgccggcatt ggcgcgggct
      181 ggctgctggg cagcgccgtg ggcgactggt ggctgttcgg ccggctgggc tggagcagcg
      241 gcggcaagtg gctgatgtcg ctgctcatca ccgtggtggc ctcggtgctg gccagtgtct
      301 tcttctacca gcgcagccag gcgcgctacc tgcaggcgca tatggcacgc gcccagcgcg
      361 acgcggccga ggcgcggctg aagctgctgg aggtgcagct ggagccgcat atgctgttca
      421 acaccctggc caatctgcgc gccctcatgg ccaccgatgt gccacgcgcc caggccatgc
      481 tggaccacct gatcgcctac ctgcgcgcca cgctgggcgc ctcgcgcgcc acgctgcacc
      541 cgctggagga cgagtttgcg cgcctggcgg actacctggc gctgatggcc atgcgcatgg
      601 gcccgcgcct ggtctatgcg ctggatctgc ccgagccgct gcgcggcctg ccggtgccgc
      661 cgctgctgct gcagccgctg gtggaaaacg ccatacgcca cggcctggag ccgcaggtgg
      721 cgggcgggcg catcgacgtg caggcgcgcc tgctgcaggc accagaccag gcggcgcgcc
      781 tgcgcatcga ggtgcgtgac acgggccccg gcccgggcgg cggcgaaccc ggcacggcat
      841 tcggcctgca gcaggtgcgc gaacgcctgg ccacgctgta cggcacctcg gcaactctcg
      901 aattgatagc tatcagcgaa ggtggtacaa gcgctgtcgt cacttttcct ttaaaaccag
      961 agcaaccatg acggcacccc gcgccctgat cgccgaggat gaacccctgc tggccgccgc
     1021 cctggagcaa gagctggcgc gcgcctggcc cgagctggcc attgccgtcc gcgtgggcga
     1081 tggcctgtcc gccgtgcgcc aggcgctggc cctgctgccc gacatcctgt tcctcgacat
     1141 ccgcatgccc ggctgcagcg ggctggaggc ggccctggag ctggccgacg cctggcccca
     1201 gggccagccc ttgccggccc tggtcttcgt caccgcctac gaccagtacg ccgtgcaggc
     1261 cttcgaggcc caggccgtgg actacctgct caaacccgtg cagcccgagc gcctgcgcaa
     1321 gagcgtggac aggctgcgcc aggcgctggc gcagcgcgac accgcctggg cgcaggagca
     1381 gaccctggcc cagttgcgcc agctggccca ggcgcccggc ctggacagac cgccgacagc
     1441 cgcgccgctg acgctgctgc aggccagccg cggcaaccag attcacatgg tggcgctggc
     1501 cgaagtgctg tacttcgagg ccgccgacaa atacctgcgc gtgctcaccg cgcggcagga
     1561 atacctgatc cgcaccccgc tcagggagct ggtggcgcag ctcgacccgg ccgtgttctg
     1621 gcaggtgcat cgcggcaccc tggtgcgcgc cacggccatc gccagcgccg agcgtgacga
     1681 ggccggccgc ctgtacctgc acctgcgcga ccggcccgag cggctggccg tgagccggct
     1741 ctacgccccg ctgttcaagg ccatgtaggg cgccgcattc gggtaggctt ggcgcccatg
     1801 cctgacgcca gcaccgccca caccagcgcc tcgcccgccg ccaccacgcc catgcccgcg
     1861 cgcaccgccc tgctgatgct ggtggcgctg acctcgggct ttgccctcag ctcggccttt
     1921 cgcaccgtgg ccgccatcct ggccgcgccg ctgcaggcag actttgccct gtcgccccag
     1981 gcgctggggg tgtttgccgc caccttccat ttcacgtttg gcgccatgca gctgttcatg
     2041 ggcataggca tagacctgta cggcgtgcgc cgcaccatct tggcggcgtt tccgctgtgc
     2101 attgccgggg ccgtggtgtc ggcgctggcg ccgggctatg gcgcgctgct gctggggcag
     2161 gcgctgatcg gtgtgggctg cgcgccggcc tttctggtgt gcacggtgtt catcgcgcgg
     2221 catttcgcgc cgcagcgctt tgccgccatc tcgggcgtgg ccatggcgct gggtacgctg
     2281 ggcatgctgt tcaccggcac gccgctggcc tggctggtgc agcaatggtc gtggcgcgcg
     2341 ggcttctggg tgctggccct gctggccctg ctggcctggc tgtggatctg gcgcacggtg
     2401 catgagccca ccccggacgg cacgccggag ggcccgcgcg aatcggtgcg cgaggccgtg
     2461 ctgcgctttg ccagcctgtt caccctgccc cacacctggg gcatcgtggt gctgggcgcc
     2521 gtgacctatg cggcgctgat ctcgctgcgc gggctctggc tcgggcccat gctgatagag
     2581 cgccatggct tttcgctggt gcaaagcggc aacgtggcgc tggcgctgtc ggtggtggcg
     2641 ctggccgggc ccatgctgtt tggccgcata gaccccggcc ccgccacccg gcggcgctgg
     2701 atcgtcgcct gcacgctgtt gatggcgctg ctgttcacgg ccatggccgt gctgcatgcg
     2761 gcctggctgg acatggcctg catgctgctc atcggcctgc tgtcgggcta cctcatcctg
     2821 cagtacgccg atgtgcgcgc cgcctacccg gcggcactca ccggccgagc catggcggtg
     2881 ttcaccatgg ccatgttcct gggcgtggcg gccatgcagt ggttcaccgg cctggtggcg
     2941 tcgctggcca aggcccaggc catggccgat ccgtttgtcg ccgtgatggc cagcattgcc
     3001 gcgctgctgg cgctggcggc cctgggtttt cgcctgctgc cgcagccgcc gcgtgagggc
     3061 ggcgtggggt aattccccgt gtcttgatgg gcatggcggc ccttatcatc gccgcgtcca
     3121 tatcactttt acctaaagta cgacccggca tggatcaagt tccgggcccg tgccaacact
     3181 ggagggcgcc aagacatgaa aaattccctg accgccgggc tggccctgtg cctgctcgcc
     3241 gccaccggcc tggcccatgc cagcagcgac ccgctgcagg tgcgcagctg ggccgccgcc
     3301 tgcgccaact gccatggcac gaatggccag gcacaaccgg gcaacgagcc gctggccggc
     3361 ggcaacaagg acgagatgct gaagaaactg atggacttca agagcggcgc caagcccgcg
     3421 accatcatgc accagctctc caagggctac accgacgagc agctggcggc cattgccggc
     3481 tggtttgccg cgcagaagaa ataacgggag gcagcaccat ggatcgtcgt caattcattc
     3541 gcgccggctc cgccgcatca gccgccaccg ccctgggcct gacggccggc tgcgccagca
     3601 ccggcagcgc agcgggcgcc aaggtggtcg tcgtcggcgg gggctatggc ggcgccaccg
     3661 ccgccaagta catccgcatg tggtccgagg ggcgcatcga ggtcacgctg gtcgaaccca
     3721 atgacagctt tgtctcctgc ccgctgtcca acctggtgct gggcggcagc aagcagatgg
     3781 ccgacatcac cacgccctac gacaatctga ccaggcgcca cggcgtgaag atcgcgcgcg
     3841 accgggccac cgccatcgac gccgacaagc gccaggtgcg cctcgccggt ggccagacgc
     3901 tggcctacga ccgcctggtg ctctcgcccg gcgtggactt catgtgggac gcgctgcccg
     3961 gcatgaagaa ggccggcgcg caggacaggg tgctgcacgc atggaaggcc ggcccccaga
     4021 ccctggcgct gcgcaaacag ctcgaggcca tgcccgacgg cggtgtgtat gccatcagca
     4081 tcccgctggc accctaccgc tgcccgcccg gcccctacga gcgcgcctgt caggtggcgc
     4141 attacttcag ccaggccaag cccaaatcca aggtgctgat tctggacggc aacgacgacg
     4201 tgacctccaa gggcccgctg ttcaagaagg cctgggcgga tcgctacaag ggcatcatcg
     4261 aataccgccc caagcacagc acggtggatg tcgataccgc caccaacacg ctgaagttcg
     4321 acttcaacga cgacgtcaag gcccaggtgc tgaacgtgat tccgccgcag cgcgccggcg
     4381 acattgccca caacgccggc ctgaccacgg ccaacaggcg ctggtgcgag gtggactggc
     4441 tgaccatgga gtccaccgcc gccaagaaca tccacatcct gggcgatgcg ctgcagatcg
     4501 cgccgcagat gcccaagtcc ggccatatgg ccaaccagca tggcaaggtg gcggccgccg
     4561 ccatcgtcgc gctgctgtcg gggcgtgcgc cgaatgccct gcccatctac aacaacacct
     4621 gctacagctt tgtcagcgac accgacgtgg tgcatgtggc cagcgtgcac cgctacgacg
     4681 ccgagaagaa gaccatgctc acggtgccgg gctcgggcgg cgtgtccgcg gccgccagcg
     4741 agctggaggg gcgctatggc ctggcctggg cgcgcaacat ctgggccgac accctggcat
     4801 agcctgcccc acaccatcaa cccggggcgc ccagcgcccc ttttttcatg accagagcca
     4861 agaatatgcc ttccatgcgc acacagccca tacgccacgg cctcctggcc ctggcctttc
     4921 tgagcctggc gacaagcgcc gccgcacagg gcagcgacga cgaggcgcgc gccaagaaaa
     4981 tcatcggcgg ctcctgcttt ctgtgccacg gcgccgaggg cgagtcggcc agcgccatct
     5041 acccgcgcct ggccggacag aacgccgagt acatcgccaa gcagctggag aacttcaaga
     5101 acggccagcg caagagcacg gccatggcgc ccatggtggc cagccttgct cccgaggaca
     5161 tggtggcgct gggcaagttc ttcgccagtc gcccggcgca gcaaaaaaaa gcccgggacc
     5221 aggatctggc agccgtgggc cgctacatct accaccacgg caaccgcttc agcggcatac
     5281 ccgcctgcgc cacctgccat ggcccggacg ccgccggcag cgccacgctg ccgcgcctgg
     5341 cgggccagca tgtggcctac acggaagacc agctcaagct gttcaacaag cgcgaacgca
     5401 ccaacgacaa cgccgtgatg cacgccgtgg tggagaagat gacggccctg gagatggcgg
     5461 ccgtggccga gtacctgagc ggcaagtagc gttcannnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnngccag ttgttccacg atgggcgcct gctgggtcac cggctgcaga
     5641 ctgtgaatgg gctcggccag ccagcggtcc atgaacccgg cacccagcca catgcccgcc
     5701 agcgccagcc aggcggtgag cgaaaagatg gcgccgcccg tcatgccggc ccccaccgcg
     5761 cagccgccgg ccagcatgct gccaaagccc atggccatgg cgccggcgat gtagcgcgcc
     5821 atgctgtagc cgtccttgaa gccctcgatc ttgaactccc gccccaccag cgcgcccagc
     5881 agcgagccga cgaacaccgc gggcaacatg ccggcatcga agccgaacgt cgttccgggg
     5941 ttggccagca cgcgcatcag ccattcggcc gaggggccgc tgaaggtcag ccctcccacc
     6001 ggcaccacat caaagctggc ctgcgccacc gcgtggttga accaccaggc cagggccacc
     6061 gccaggccgg tgcacagccc gcccagccac atccaggccc ggttgccgct gcgccaggaa
     6121 aagtacagcg ccaccgccag ccacagcgcg cccagcacca ggccatccca atggccggca
     6181 cccaccaggg ccagcaggtt gcgcgcgctg ccgccctcca ccgtccacca gccggcgatc
     6241 tcgctgcgca gcggcgccag gctgccggcc agcgtggcct gcgccgtcac ggcaaacacc
     6301 agcccggaca gcagggcgcg cagattgccg ttggccgaca ggatgagcag gcggctggcg
     6361 cagccacgcg tcatcaccat gcccacgcca aacagcaggc cgccgaccag cgcgcccgac
     6421 aggctgccgc ggttggccag ctggcgcacg ccgctcacgt ccagccagcc ggccagaatc
     6481 aagccctgca ccgcgatcag cgccgtggca aacgccagca gccagacgga gagcttctcg
     6541 gcaaacttgc cgcgccagaa ctcgatcacc gccgcacgca ggcaaaaccg cgagcgctgg
     6601 gcaaaaaaac cgaacagcag gccgatcacg gccccgccca ccgagagcac accggccgtg
     6661 ccatgccgct ccaccaaggt tgccaagtcg atggtcattt ttgataatca attagctctt
     6721 actaataaac gcgggagaca tcatgcaacc aacaccgagc cgacgccaga ttttgcaagg
     6781 ctgcgcctgc ctgggcctgg gtggcatgct cggcggccgc tgggcgggct atgcccaggc
     6841 ggctgacaaa ccgcccgcgg cacccgccat gcaggcgcac gaggcggcgc cgggctgctg
     6901 gtatgtcgaa ggcctgtcgg ccctgggctc gccggccaac cagaacttta tctccaacgc
     6961 cggcttcatc gtcacctcga ccagcgtggt ggtggtggac gccctgggct cgccggcgct
     7021 ggccgagcgg ctgctggccg agatcgcccg ggtgacggac aagccggtca gccatgtcat
     7081 cgtgacgcat taccacgccg accatgtcta tggcctgcag gtgtttgccg accggggcat
     7141 accaatactg gcgcaccagg ccgggcgtga atatctgcac gccgacaccg cgcgcctgcg
     7201 gctgcaggcc tcgcgcgagg agctggcgcc ttggatcaat gacaagacac ggctggtgcc
     7261 ggccacgcaa tgggtcgatg gccgccagga gctgaccgtg ggcgacacgc agctggtgct
     7321 gcagcccgtc ggcccggcgc acacgccgga agacctggcc gtctacctgc cgcagcgcaa
     7381 ggtcttgtac gccggcgacc tggtgtttcg cagccgcatn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnc aactacctgg cccccatgca tgcgcgcgaa ggcctggccg
     7561 tggtgcaggt ggacatgcgc ggcacgcagg tcacgcgcaa cctgcagggc cagcccagca
     7621 cgcatgacgc gctggtgcgc gcctggcagg tgaaggccgc gcccacggtg ctgttcctgg
     7681 ggcctggcgg ccaggaggtg gcggaccggc tggtgggcgg ctcgcccgac ttttactcgg
     7741 cctacctgga cggccggctg gagcgggcgc gccaggccct gggcaagtga tgattcatca
     7801 tgtttttggc atcaaaagct tatgcatcaa gcgccaaaag ctatcaatta aatagtaaaa
     7861 aggagatacc atgccacccc tgacctcgca atggctgctg ctgctggccc tgggcatggg
     7921 ctcggccgca tcggcacagg agcccgccct gttcaagaac gccgacctga agctgggcca
     7981 gcggctgatc gccgagaaca aatgcgccga atgccatgcg cgcaaatggg ccgacgatgg
     8041 caacgccatc tatcgcccca agggccgcat caacacaccg gcgctgctgc agggcatggt
     8101 cgagcgctgc agcagcgagc tcagcctgaa cctgtttccc gaggagatca cggccatttc
     8161 cgcagcgctg aaccacgcgc attacaagtt ccgcgatgcg ccctgacgcg cccacccggg
     8221 gcgcaacccg ggcgggcgtt gcactggctt actcgatcgc caggcgctgc gtcttgttgc
     8281 cctgcttctt gggcagcgtg agggtcagca cgccattgtc gtagcgcgcc ctggcctggg
     8341 cggcgtccac ctccaccggc agctgaaagc tgcgggcgac cgagccgtag tagcgctcgc
     8401 tgcgcagcag cttctcgcca tccttcttct ggtcatgctg gcgcacctcg gcgcgcaggc
     8461 tgaccacgtt gccgtcgatg gagacgttga tgtcctcctt gggcacgccc ggcacctcgg
     8521 catgcacggt ataggcggcg tcgtcctcct tcacgtcgat cttgatctgg gacggctcgg
     8581 gcagcgcgtc gccatgcagc ggacgcacat agaaggcggg cgcaaagtcc ttgaagaaat
     8641 cgtcgaacaa accgctgcgg ctgaccagtg cattcatgat gaaactcctt ccttgacacg
     8701 ttgagataaa gagcccctgg gggccatcgg gcaagcgaat cctgcccgtt gccatctata
     8761 taggccccca tgcccggggt tcaagggcac agagtccagg ggtttgatat ggcgcaaacc
     8821 cggccatggg cggggacaat cagccccatg cttgccgccc tgctcagcag cttctcctgg
     8881 cgcgcgctgc gccaccaccc ctggcgcagc gcagccgccg tggccgccgt catgctgggc
     8941 gtggcgctgg gctttgccgt gcatgtgatc aacgcgtcgg cgctgggcga gttcagccag
     9001 gcgatgcgct ccgtgggcgg ccagccggat ctggagctgc gcgccacgcg cggccatcta
     9061 ccagagtcgc tctacggcct ggtcgccgcc cagccccagg tggccctggc cagcccctgg
     9121 ctggagctgc cggtgcgcgc cagcagcgcc gccgcaccgg atggcgtgcc actgcgcctg
     9181 ctgggcgccg acgcgctgct gctgccggcc atggccccgg cgctggcgcc acgcccgcat
     9241 gcgggcgcag accgcctggc catgctggcg ccggccacgg tatttctgaa cgcggcggcg
     9301 cagcaggccc tggggctggc aagcatcgat gccgacagcc ccgccctcac gctgcacgcc
     9361 ggcctgcagc agcatctggt gcgggtggcg ggcgtggtgg cggcgggcgg cgcgcccctg
     9421 accgtgatgg acattggcgc ggcgcaggac ttatttggcc gccagggcca gctcacgcgc
     9481 atcgacctgc gcctgcgccc gggccacacg cgcgcgcagc tgcaagaggc gctggccgcc
     9541 ctgcccggct ggcccgcggg cgtgcagttt gccgagccac aggacgccct gcagcgcgtg
     9601 gacagcctct cgcgcgccta ccgcgtgaac ctcacggtgc tggccctggt ggcgctgttc
     9661 acaggcgcct tcctggtgta ttcggtgctg gcgctcagcg tggcgcagcg cgcgccgcag
     9721 ctggccctgc tggccgtgct gggcgccacg ccgcgcgagc ggctggcgct ggtgctgctg
     9781 gagtcggccg cgctgggcgt ggtgggcagc ctggccggca ttgccctggg cacgctgctg
     9841 gcggcgctgg cgctggcgc
//
LOCUS       DAOMTQ010000113         9815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1377_length_9815_cov_25.204918, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000113 DAOMTQ010000000
VERSION     DAOMTQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1377_length_9815_cov_25.204918"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..544)
                     /gene="rpsL"
                     /locus_tag="PK518_11065"
     CDS             complement(167..544)
                     /gene="rpsL"
                     /locus_tag="PK518_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRM49499.1"
                     /translation="MPTINQLVRQGRTVEVVKSKSPAMENCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKKPKAK"
     gene            complement(675..1838)
                     /locus_tag="PK518_11070"
     CDS             complement(675..1838)
                     /locus_tag="PK518_11070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRM49500.1"
                     /translation="MKSLLPSLRSLACAALMAPAILWAQAPQPPEIAARNYLLVDVTA
                     GQVLASKDIDAPVEQASLTKLMTGYLVFDALRAKKITLEQKLPVSERAWKMPGSRMFI
                     DPKMQVPVDDLLKGMIVQSGNDATMALAEGVGGTAENFVRLMNEQAKALGMTGTSYKN
                     PEGLTEPGHTTTAKDLATLATRLMQDFPQYMHYYATKQYRYEGTPASNSSNRNALLFR
                     DPTVDGLKTGHTAAAGYCLVATAKRDFPNLGQRRLLSIVLGAASENARANESQKLLNW
                     GYTAFDSVKLFDAGQAAATPAIWKGTQSTLKIGREQAIVITVPSGSAGKVSTEIVRQD
                     PLLAPFTKGQPVGTLRVKLGEQQVAELPLVALEDVGQAGIFGRTWDAIRLWIK"
     gene            complement(1921..2556)
                     /locus_tag="PK518_11075"
     CDS             complement(1921..2556)
                     /locus_tag="PK518_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM49501.1"
                     /translation="MNAQTERLSLAGPAGAIEVARDAAAAGVAPRGVAVIAHPHPLFG
                     GTMDNKVVQTLARAFVSGGFAAVRFNFRGVGASAGVHDDGQGELDDLLAVVQHMAPEG
                     PIALAGFSFGAFVTSHALARLWSEREVVAAVLVGTAASRFTVAPVPPEAHLRTLVVHG
                     EHDETVPLTSVMDWARPQTLPVTVVPGGGHFFHGQLPLLRSLATRHLQAMA"
     gene            complement(2630..2971)
                     /locus_tag="PK518_11080"
     CDS             complement(2630..2971)
                     /locus_tag="PK518_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRM49502.1"
                     /translation="MSETTPQYYQRHIFFCLNERTNGENCCAQHGAQQAFEHCKALVK
                     QQGLAGAGKVRVNKSGCLDRCAGGPIAVVYPEGIWYSYVDIADIEEIVESHLKNGVVV
                     ERLLTPPELGR"
     gene            complement(2983..4080)
                     /locus_tag="PK518_11085"
     CDS             complement(2983..4080)
                     /locus_tag="PK518_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM49503.1"
                     /translation="MHKTSAWPLALIYAALIVFASLFPFEGWREQGVSPWVFLTARMP
                     PPYWTWFDVNLNLAGYAPLGFLLALALLRTHWPRAAVPAAALAGALLSLSMEFLQIYL
                     PRRVPSNMDLGLNAAGALLGALVAALLERLGAISHWSRFRERWFVPQARGALVLLALW
                     PWALLFPAALPLGLGQVWERLEAALADVLEGTPFSAWLPAVDLALEPLSPGGELLAVA
                     LGLLIPCLLGFCIMRHMGRRMLLALSALAVGVAATALSALLSYGPAHAWEWLTPATAI
                     GLGVGLAAALGLATLPRRGCAAVLLLVLMLHLNLLNQAPTNAYFAQTLQAWEQGRFIR
                     FYGLGQWLGWLWPYAALLYVLLRVSRRDVQS"
     gene            complement(4097..4525)
                     /locus_tag="PK518_11090"
     CDS             complement(4097..4525)
                     /locus_tag="PK518_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRM49504.1"
                     /translation="MLWVKTFHIVFIASWFAGLFYLPRIFVNLAMVAPGSVAERDRLL
                     LMARKLLRFTTLLAVPAIGLGLWLWLGYGIGQGAGNGWLHAKLGVVVLIVLYHWICAR
                     MLRALAANTDQHSHRWFRWFNEVPVLLLVVAVALVVVKPF"
     gene            complement(4694..5398)
                     /locus_tag="PK518_11095"
     CDS             complement(4694..5398)
                     /locus_tag="PK518_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HRM49505.1"
                     /translation="MTEPPSPLSTIAQEQAWNRQQMLEPVTRPRFAVWQYEAPAIVLG
                     CSQRKFEDGARARLGGAAELLLRPSGGGAVLTGPWMVSCSVVLPLDHPWVQGRLPDSY
                     HGLGQLHVDLLSNLGVPSTALPPDQVDACNARTGPVVPWACYGSLAPWEVVDAAGRKL
                     VGLAQRRQRTGVLLVAGTLVTPPDWALLCSAMGQPQDEAAMCRRTVDCTQLSATPPDA
                     RQLARALQQALEQALG"
     gene            5456..6463
                     /gene="hemB"
                     /locus_tag="PK518_11100"
     CDS             5456..6463
                     /gene="hemB"
                     /locus_tag="PK518_11100"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRM49506.1"
                     /translation="MHLSSPTPFPANRPRRLRRDAFTRNLVRENMLTPHDFIYPVFVH
                     EGSKLAVPVPSMPGVQRLSLDLLLPVAEECVKLGVPYLALFPSIDAALKTPDGKEALN
                     PEGLIPRVVRALKKEFPQLGVMTDVALDPYTSHGQDGVLDDTGYILNDETVEILTGQA
                     LTHAQAGVDMVAPSDMMDGRIGAIREALEAHGLIHTRIMAYSAKYASAFYGPFRDAVG
                     SSANLGASNKDVYQMDPANSDEALREVALDIAEGADMVMVKPGMPYLDVLRRVKDEFK
                     VPTFAYQVSGEYAMLKAAAANGWLDHDKVMLEALLAFKRAGADGILTYFALDAARLLK
                     K"
     gene            6503..7675
                     /locus_tag="PK518_11105"
     CDS             6503..7675
                     /locus_tag="PK518_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRM49507.1"
                     /translation="MTENTLQPIRVFHLQPGSGAQELAGLPQAPPAQGFYWIACTRAA
                     FSAELPRLQAALQTLAGQQLVDLHVSDLLNAQLPSHYDYTAQYDLLVVRRLAQAQAAG
                     PAPLRTSARGGPPVLRRIDTSPVGFAVFDQVLLSVHPEDCGVRDAYAARLVTPMAAAA
                     ISATAAEAVVHELRSGPVAGTRLPPSPADLMLRVVNHIVDNYLDLRRELSRQLDHWQA
                     ELLRPRTRFGNWGALLDARLNLHQLDEICEDQRTAVRAWLEALDSWSEPEGSAAALRE
                     LDLLKVRSRDVLEHIERVVTHVRRLEHSTETAVQMHFSVQSNRTNDIMRTLTALTAVF
                     LPLNLIAGIFGMNFEFLPLIHQQHGFWWAMAAMGLISITLVALFWRKRYLARTGQG"
     gene            7787..9127
                     /locus_tag="PK518_11110"
     CDS             7787..9127
                     /locus_tag="PK518_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRM49508.1"
                     /translation="MKRIKTSLFGLFAGMTALWLVAEAPALAAAKGVFQWRHLLVQYT
                     GLLAMGAMSVAMLLAVRPAWLEPRLHGLDKMYRLHKWLGVAGLVLAITHWLWAQGPKW
                     LVQAGLLTRPGRGPRAPEALEILRLLRSLRGLAENLGEKAFYIAVILIALALIKRFPY
                     RHFFRTHRWLALVYLVLVFHSVVLLDFAYWRQPVGWAMGLLLASGSVAAGISLLRRIG
                     AQRKALGEIVRLDYLDAVQVHAVTLQLKTPWAGHEAGQFAFVTFDEAEGAHPFTMSSA
                     WTGDGRLLFLIKALGDYTRTLGHALRVGDTAIVEGPYGRFHFEGSAQRQIWIGAGIGV
                     TPFVARMQALAAQPDGRSVDFFHVTAEVDERALALLRADAQAAGVRLHLVVSRRGDTD
                     SLFTGERLRAQVPDWLQADVWFCGPANFGDALQQDLVAHGLAPGHFQRELFEMR"
     gene            complement(9163..9702)
                     /locus_tag="PK518_11115"
     CDS             complement(9163..9702)
                     /locus_tag="PK518_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRM49509.1"
                     /translation="MTTLQWSEDMGVEMAQMDATHQECVALLQAVQGADDARLMAAWE
                     RLLAHTEAHFAEEDRWMQATRFAAGNCHSQQHQQVLNIMRDGTERARKGRLVELRLMA
                     AELAVWLPQHIDAMDAGLAHHLRQVGYDPATGHIAHPEALPHEPLQGWGGASGLGEGD
                     APVAGQVAGCSDKKMGCNA"
BASE COUNT         1631 a         3396 c         3290 g         1498 t
ORIGIN      
        1 acctctatct ttgcttcagt tggcgccgtt tccggacacc gcgagagcca tgatggactc
       61 ccacttactc gacccacgca aaccattttg cgcttcgggc cactacgctg cacaccgcgc
      121 cacgcggcaa ggcagcacca aaaattcaaa cccgaagaac cagggcttac ttggccttgg
      181 gcttcttggc accatacttg gagcgcgact gcttgcgatc cttgacgcct tgcaggtcga
      241 gcgaaccacg cacgatgtgg taacgcacac cgggcaagtc cttgacccgg ccgccgcgca
      301 ccagcaccac gctgtgctct tgcaggttgt ggccttcacc gccgatgtag gagatgacct
      361 cgaagccatt ggtcaggcgc accttggcga ccttacgcag agcggagttg ggcttcttcg
      421 gcgtcgtggt gtacacacgg gtgcacacgc cacggcgctg agggcagttt tccatggcag
      481 ggctcttgga tttgacaact tcgaccgtgc ggccctggcg cacgagttgg ttaatggttg
      541 gcattaatac gtccctaaac gtgataaaac gaaaacgtga gtcccttcgg aaattccgaa
      601 aagccttcta atgtatcagc ttgcccaggc gcaggcaatg gctttgtcaa aagccccacg
      661 cgtgcgccgc cggcttactt gatccagagg cgtatagcgt cccaggtgcg gccgaagatg
      721 cccgcctggc ccacatcctc cagggccacc aggggcagct cggcgacctg ctgctcgcca
      781 agtttgacgc gcagcgtgcc caccggctgc cccttggtga agggagccag cagcggatcc
      841 tggcgcacga tctcggtgct gaccttgccg gcgctgcccg agggcacggt gatcacgatg
      901 gcctgctcgc ggccgatctt gagcgtgctc tgcgtaccct tccagatggc gggcgtggcc
      961 gcggcctggc cggcgtcgaa cagtttgacc gagtcgaacg ccgtgtagcc ccagttcagg
     1021 agtttctggc tttcattggc gcgcgcgttc tcgctggccg cgcctagcac gatggatagc
     1081 aaacggcgct ggcccaaatt gggaaagtcg cgcttggccg tggccaccag gcagtagccc
     1141 gccgccgccg tatggccggt cttcaggcca tccaccgtcg ggtcacgaaa cagcagcgca
     1201 ttgcgattgc tgctgttgga ggccggcgtg ccctcgtagc ggtactgctt ggtggcgtag
     1261 taatgcatgt actgtggaaa gtcctgcatc aaccgcgtgg ccagggtcgc cagatccttg
     1321 gcggtggtgg tgtggccggg ctcggtcagg ccctcggggt tcttgtagct ggtgcccgtc
     1381 atgcccaggg ccttggcctg ctcgttcatc aggcgtacga agttctccgc cgttccaccg
     1441 acgccctcgg ccagggccat ggtggcgtcg ttgcccgact gcacgatcat gcccttgagc
     1501 aggtcatcga ccggcacctg catcttgggg tcgatgaaca tgcgcgagcc gggcatcttc
     1561 caggcgcgct cgctcaccgg cagcttctgc tccagcgtga tcttcttggc gcgcagggcg
     1621 tcgaacacca ggtagccggt catcagcttg gtgagcgagg cctgctccac cggcgcgtcg
     1681 atgtccttgg aggccagcac ctggccggcc gtcacgtcca ccagcaggta gttgcgcgcg
     1741 gcgatttccg gcggctgggg cgcctgggcc cagaggatgg ccggagccat cagggcggcg
     1801 caggcgaggg aacgcaatga gggcaggaga gacttcatgg tgggagcggg tacttcaaac
     1861 aactgaaagc gacataccga ccgcggcggc cggtatgcgt gtggaaatga gggaggtgcg
     1921 tcaggccatg gcctgcaagt ggcgcgtggc caggctgcgc agcagcggca attgtccgtg
     1981 aaagaaatgt ccgccccccg gcacaaccgt aaccggcagt gtctgcggcc gggcccagtc
     2041 catcacgctg gtcagcggca cggtttcatc atgctcgcca tgcacgacga gggtgcgcag
     2101 atgcgcctcg ggcggcaccg gcgccaccgt gaagcggctg gcggccgtgc ccacgagcac
     2161 ggctgcgacc acctcgcgct cgctccacag ccgcgccagc gcatggctgg tgacgaaggc
     2221 gccaaacgaa aaaccggcca gggcgatcgg cccctcgggc gccatgtgct gcaccacggc
     2281 cagcaggtcg tcgagctcgc cctggccgtc gtcatgcacg ccggccgagg cgcccacgcc
     2341 gcggaagttg aagcgcacag cggcaaagcc gccggagaca aaggcgcgcg ccagggtctg
     2401 cacgaccttg ttgtccatgg tgccgccaaa cagcggatgc gggtgggcga tcacggccac
     2461 gccgcgcggc gccacgccgg cggccgcggc gtcgcgcgct acctcgatgg cgccggcagg
     2521 gcccgccagg ctcaggcgtt cggtctgggc attcatgttt gctatttaat caagagctta
     2581 ttgcacttta ccatcaagcg ttacaggcca aaatctttat gaataatgat caacgcccaa
     2641 gttccggcgg cgtgagcaaa cgctccacca ccacgccgtt cttcaggtgc gactcgacga
     2701 tctcctcaat gtcggcgatg tccacatagc tgtaccagat gccctcgggg tagaccacgg
     2761 cgatggggcc gccggcgcag cgatccaggc aaccggactt gttcacccgc accttgcccg
     2821 cgccagccag accctgctgc ttgaccagcg ccttgcaatg ctcgaacgcc tgttgcgcgc
     2881 cgtgctgggc gcagcagttc tcgccattgg tgcgctcatt caggcaaaag aagatgtggc
     2941 gctggtagta ctggggggtg gtttcgctca tgggtggcaa ttttaggatt gcacgtcgcg
     3001 ccgcgacacg cgcagcagca catacagcag cgcggcgtag ggccagagcc agcccagcca
     3061 ctggcccagg ccatagaagc ggatgaaacg cccctgctcc caggcctgca gggtctgcgc
     3121 aaagtaggcg ttggtgggcg cctggttgag caggttcagg tgcagcatca gcaccagcag
     3181 cagcaccgcc gcgcagccgc gccggggcag cgtggccaac cccagggccg ccgccaggcc
     3241 cacgcccagg ccgatcgccg tggccggcgt gagccactcc caggcatgcg ccgggccgta
     3301 gctgagcaag gcggacagcg ccgttgccgc cacgccaacg gccagcgccg acagcgccag
     3361 cagcatgcgc cgccccatgt ggcgcatgat gcagaaaccc agcaggcagg ggatgagcag
     3421 ccccagagcc acggccagca gctcgccccc aggcgacagc ggctccagcg ccaagtccac
     3481 ggccggcagc caggcgctga acggtgtgcc ctccagcaca tccgccagcg ccgcctccag
     3541 ccgctcccac acctgcccca ggcccagtgg cagggccgcc ggaaacagca gcgcccaggg
     3601 ccacagcgcc agcagcacca gcgcgccacg cgcctgcggc acgaaccagc gctcgcgaaa
     3661 ccggctccag tgcgagatcg cccccaggcg ctcgagcagc gccgccacca gcgcgcccag
     3721 cagcgcgccg gcggcgttga gccccagatc catgttggag ggcacgcgcc gcggcagata
     3781 gatctgcaaa aactccatgg agagcgacag cagcgccccg gccagcgccg ccgccggcac
     3841 ggccgcgcgc ggccagtgcg tgcgcagcag ggccagcgcc agcagaaagc ccaggggcgc
     3901 gtagccagcc agattcaggt tgacgtcaaa ccaggtccag tacggcggcg gcatgcgcgc
     3961 cgtgaggaac acccagggcg acacgccctg ctcgcgccag ccctcgaagg gaaacaggct
     4021 ggcaaagacg atcagcgcgg catagatgag cgccaggggc caggccgatg tcttgtgcat
     4081 ggcgcagcgg ccagcctcag aaaggcttga ctaccaccag cgccacggca acgaccagca
     4141 gcagcaccgg aacctcattg aaccagcgaa accagcggtg gctatgctgg tcagtgttcg
     4201 ccgccagggc acgcagcatg cgcgcgcaaa tccagtggta cagcacgatg agcaccacca
     4261 cgcccagctt ggcatgcagc cagccgttgc cggccccctg ccctatgcca tagcccagcc
     4321 acagccacag gcccaggcca atggcgggca cggccagcag tgtggtgaag cgcagcaact
     4381 tgcgcgccat gagcagcagc cggtcgcgct cggccaccga gcccggcgcc accatggcca
     4441 ggttgacgaa gatgcgcggc aggtagaaca ggccggcaaa ccagctggcg atgaagacga
     4501 tgtgaaaggt tttgacccag agcatcggcg cagtgtacgc gccgggttcg gccaaaaaag
     4561 aggcccggac ggagccgggc tggaaagcct tgcgacgatc aacaccgcag ggccccgctc
     4621 agggaggtaa agcggggggc agccaggccg cccgggcgca atctagcatt ggcgggcggt
     4681 gggcgcaagc gcactagccc agcgcctgtt ccagcgcctg ctgcaacgcc cgcgccagct
     4741 ggcgcgcatc gggcggcgtg gcgctgagct gggtgcaatc caccgtgcgc cggcacatgg
     4801 cggcctcgtc ctggggctgc cccatggcag agcacaacag cgcccagtcg ggcggcgtga
     4861 ccagggtgcc ggccaccagc agcacgccgg tgcgctggcg ccgctgggcc aggccgacga
     4921 gcttgcggcc ggcggcgtcc accacctccc agggcgccag gctgccgtag caggcccagg
     4981 ggacgacggg gccggtgcgc gcgttgcagg catcgacctg atcgggcggc agggccgtgg
     5041 agggcacgcc caggtttgag agcagatcca catgcagctg gcccaggccg tggtagctgt
     5101 ctggcaggcg gccctgcacc caggggtgat ccagtggcag caccaccgag cagctgacca
     5161 tccacggccc ggtgagcacc gcgccgccgc ccgaggggcg cagcagcagc tcggccgcgc
     5221 cgcccaggcg cgcgcgggcg ccgtcctcga acttgcgctg cgagcagccg agcacgatgg
     5281 ccggcgcctc gtactgccac acggcaaagc gcggccgggt cacgggctcc agcatttgct
     5341 ggcggttcca ggcctgctcc tgggctattg ttgaaagggg gctcggtggc tcggtcatgg
     5401 gcgcgaaggc tatcaccgga ccgtgggcgc gcgcaggtgg gggacaatcg ccgtcatgca
     5461 tctgtccagc cccaccccct tccccgccaa ccgtccgcgc cgcctgcgcc gcgacgcctt
     5521 tacccgcaac ctggtgcgcg agaacatgct cacgccgcac gacttcatct acccggtgtt
     5581 cgtgcacgag ggcagcaagc tcgccgtccc cgttccctcc atgcccggcg tgcagcgcct
     5641 gagcctggac ttgctgctgc ccgtggccga ggaatgcgtc aaactgggcg tgccctacct
     5701 ggcgctgttc ccgtcgatag acgctgcgct gaaaaccccc gacggcaagg aggcgctgaa
     5761 ccccgagggc ctgatcccac gcgtggtgcg cgccttgaag aaggagttcc cgcagctcgg
     5821 cgtgatgacc gacgtggcgc tcgaccccta caccagccac ggccaggacg gcgtgctgga
     5881 cgacaccggc tacatcctga atgacgagac cgtggagatc ctcaccggcc aggccctcac
     5941 ccacgcccag gccggcgtgg acatggtggc gcccagcgac atgatggacg ggcgcatagg
     6001 cgccattcgc gaggcgctcg aggcgcacgg cctgatccac acgcgcatca tggcctacag
     6061 cgccaagtac gccagcgcct tctacgggcc gttccgcgac gccgtgggct ccagcgccaa
     6121 cctgggcgcg agcaacaagg acgtctatca gatggacccg gccaacagcg acgaggccct
     6181 gcgcgaggtg gcgctggaca ttgccgaggg cgccgacatg gtcatggtga aacccggcat
     6241 gccctacctg gacgtgctgc gccgcgtgaa ggacgagttc aaggtgccga ccttcgccta
     6301 ccaggtgtcg ggcgaatacg ccatgctcaa ggccgccgcc gccaacggct ggctggacca
     6361 cgacaaggtg atgctggagg cgctcttggc cttcaagcgc gcgggggccg acggcatcct
     6421 gacctacttt gcgctggacg ccgcacgcct gcttaaaaaa taatagctgc tcgcgcttga
     6481 tacaaaagcg ctggaggccg atttgactga aaacacgcta cagccgatcc gcgtcttcca
     6541 tctgcagccg ggcagcggcg cgcaagagct ggcgggcctg ccccaggcgc cgccggccca
     6601 gggcttttac tggatcgcct gcacgcgcgc ggccttcagc gccgagctgc cgcgcctgca
     6661 ggccgcgctg cagacactgg ccgggcagca gctggtggat ctgcatgtgt ccgacctgct
     6721 caacgcccag ctgccatcgc attacgacta caccgcgcaa tacgacctgc tggtggtgcg
     6781 ccgcctggcc caggcacagg ccgccggccc cgcgccgctg cgcaccagcg cccgtggcgg
     6841 cccacccgtg ctgcggcgca tagacaccag cccggtgggc tttgccgtgt tcgaccaggt
     6901 gctgctgtcg gtacaccccg aggactgcgg cgtgcgcgac gcctatgccg cgcgcctggt
     6961 cacacccatg gccgccgcgg ccatcagcgc caccgcggcc gaggccgtgg tgcacgagct
     7021 gcgcagcggc cccgtggccg gcacgcgcct gccgcccagc ccggccgacc tgatgctgcg
     7081 cgtggtcaac cacatcgtcg acaactacct ggatctgcgc cgcgaactct cgcgccagct
     7141 cgaccactgg caggccgagc tgctgcgccc gcgcacgcgc tttggcaact ggggggcgtt
     7201 gctggatgcg cgcctgaacc tgcaccagct ggacgaaatc tgcgaagacc agcgcaccgc
     7261 cgtgcgcgcc tggctggaag cgctggacag ctggagcgag cccgagggca gcgccgctgc
     7321 cctgcgcgaa ctggatctgc tcaaggtgcg cagccgcgac gtgctggaac atatcgagcg
     7381 cgtggtaacc catgtgcgcc gcctggagca cagcaccgag accgccgtgc agatgcactt
     7441 ctcggtgcag agcaaccgca ccaacgacat catgcgcacg ctcacggcgc tgaccgccgt
     7501 gttcctgccg ctgaacctga tcgccggcat cttcggcatg aacttcgagt tcctgccgct
     7561 catccaccag cagcacggct tctggtgggc catggcggcc atggggctga tctctatcac
     7621 cctggtggcg ctgttctggc gcaagcgcta cctggcgcgc acaggccagg gttgatcccc
     7681 tgccccagcc acgcgccgtc ggtgcaagcc acccaggctt gacgcagcgc aagccccggc
     7741 ggcggctggc ttccagagag caccaaggca tgccaccatg gccggcatga aacgcatcaa
     7801 gaccagtctg ttcggcctgt tcgcgggcat gaccgcgctg tggctggtgg ccgaggcgcc
     7861 tgcgctggcc gcggccaagg gggtatttca gtggcgccac ctgctggtgc agtacacggg
     7921 cctgctggcc atgggggcga tgtccgtggc catgctgctg gcggtgcgcc cggcctggct
     7981 ggagccgcgc ctgcatgggc tggacaagat gtaccggctg cacaaatggc tgggcgtggc
     8041 gggcctggtg ctggccatca cgcattggct gtgggcccag gggccgaagt ggctggtgca
     8101 ggccgggctg ctcacccgcc cgggacgcgg gccgcgcgca cccgaggcgc tggagatact
     8161 gcgcctgctg cgcagcctgc gcggcctggc cgaaaacctg ggcgagaagg ccttctacat
     8221 cgccgtgatc ctgatcgcac tggcgctgat caagcgcttt ccgtaccgcc atttctttcg
     8281 cacccaccgc tggctggcgc tggtgtatct ggtgctggtg ttccatagcg tggtgctgct
     8341 ggactttgcc tactggcgcc aacccgtggg ctgggccatg ggtctgctgc tggcgtcggg
     8401 cagcgtggcc gccggcatca gcctgctgcg gcgcataggc gcgcagcgca aggcgctggg
     8461 cgagatcgtg cggctcgact acctcgacgc cgtgcaagtg catgccgtca ccctgcagct
     8521 caagaccccc tgggccggcc atgaggccgg gcagtttgcc tttgtcacct ttgacgaggc
     8581 cgagggcgcg cacccgttca ccatgtcatc ggcctggacg ggcgacgggc ggctgctgtt
     8641 tctcatcaag gcgctgggcg actacacgcg cacactcggg catgcgctgc gcgtgggcga
     8701 cacggcgatc gtcgagggcc cgtacgggcg ctttcatttc gagggcagcg cgcagcgcca
     8761 gatctggatc ggcgcgggca ttggcgtcac gcccttcgtg gcgcgcatgc aggcgctggc
     8821 ggcgcagccg gacggccgtt cggtggactt cttccatgtc acggccgagg tggacgagcg
     8881 cgccctggcc ctgctgcgcg ccgacgccca ggccgcgggc gtgcgcctgc acctcgtcgt
     8941 ctcgcggcgc ggcgacacgg acagcctgtt caccggcgag cgcctgcgcg cccaggtgcc
     9001 ggactggctg caggccgatg tctggttctg cggcccggcg aactttggcg atgcgctgca
     9061 gcaagacctg gtggcgcatg ggctggcgcc cgggcatttc cagcgcgagc tgttcgagat
     9121 gcgttgaata ctatttaatt aatagctgct aacgcttact catcaagcgt tacagcccat
     9181 ttttttatca gaacaccccg ccacctgacc ggccacgggc gcatcgccct cgcccaggcc
     9241 gctggcgccg ccccagccct gcaggggctc atgcggcaac gcctcggggt gggcgatatg
     9301 gccggtggcg gggtcgtagc ccacctggcg caggtggtgg gccaggccgg cgtccatggc
     9361 gtcgatatgc tgcggcagcc atacggccag ctcggcggcc atgaggcgca gctccaccag
     9421 gcggcccttg cgcgcgcgct cggtgccgtc acgcatgatg ttcagcacct gctggtgttg
     9481 ctggctgtgg cagttgccgg cggcaaagcg cgtggcctgc atccagcggt cttcctcggc
     9541 gaaatgcgcc tcggtatggg ccagcagccg ctcccaggcg gccatcagcc gcgcgtcgtc
     9601 ggcgccctgc acggcctgca gcagcgccac gcattcctgg tgcgtggcgt ccatctgggc
     9661 catctcgacg cccatgtcct cggaccattg caatgtcgtc atgcggctgt ccatgcttca
     9721 tcgcccctgc tggcgcagcc aggcggccag ctcgtcggcc agctgggccg tcgcctcggc
     9781 cagggcgcgc gcgccgccgg cggcgtcggc gctgg
//
LOCUS       DAOMTQ010000114         9811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1379_length_9811_cov_24.733600, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000114 DAOMTQ010000000
VERSION     DAOMTQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1379_length_9811_cov_24.733600"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="selB"
                     /locus_tag="PK518_11120"
     CDS             complement(<1..756)
                     /gene="selB"
                     /locus_tag="PK518_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRM49510.1"
                     /translation="MIVGTAGHIDHGKTTLVRALTGVDTDRLPEEKKRGISIELGYAY
                     LDTPAGGRIGFIDVPGHERLVHTMVAGASGIDYALLLVAADDGPMPQTHEHLAVLSLL
                     GIARGAVVITKTDRASPDRLQALRAEMARLLQGTSLAGAPVVNVSAQTGSGIAPLRQL
                     LFDAAQGQPQAAAAGAAQGLRLAIDRAFTLDGTGTVVTGTIHAGSVRVGDELAISTSG
                     GGAPLRARVRSLHAQNQGVQQAHAGQRCAVALAG"
     gene            complement(768..2237)
                     /gene="selA"
                     /locus_tag="PK518_11125"
     CDS             complement(768..2237)
                     /gene="selA"
                     /locus_tag="PK518_11125"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRM49511.1"
                     /translation="MKLPEESVVHGSPAPASPPPAAQPQNLPALDRLLRLPACAALLD
                     AHGHTLVANEARALLATLRAQALAGDLALGQIGADALAGALAERLQRRLQPAMRAVLN
                     LTGTVIHTNLGRALLPQAALAHIQAMMASPNNLEYDLASGSRGDRDSIVEGLLCELTG
                     AEAATVVNNNAAAVLLTLAALAPGKEVIVSRGELVEIGGAFRMPDVMASAGARLVEVG
                     TTNRTHAQDYERAIGEHTALLMKVHTSNYAVQGFTSAVDEARLAPIAHARGLPLATDL
                     GSGALIDMSAYGLPPEPTPQQMLAAGCDLVTFSGDKLLGGPQAGLIVGRKDLLTRIRK
                     HPMKRALRMSKLPLAALEATLRLYLRPERLAQDLPTLRLLTRPLADITATAQAVLPAL
                     QAALAPRYQVQPVALQGQIGSGSLPVERLASSGLAIAPVDAKGVGRALAQLAEALRAL
                     PLPVIGRVQDDRLLLDMRCLEDAQVFIGQIGLLRLSDKR"
     gene            complement(2237..3232)
                     /gene="fdhE"
                     /locus_tag="PK518_11130"
     CDS             complement(2237..3232)
                     /gene="fdhE"
                     /locus_tag="PK518_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HRM49512.1"
                     /translation="MNSSPTHRQINSPEDIKQRSGLEVPPLLLPDPGKLFAERALRLR
                     QLAAGHAMRDYLMLMAVVCEAQHARLQQYPEVTLPDAAQAAAAGESGEPLLAAEHWPR
                     AAQWRTELRALLRQVLDKLPGDSPARAGVQAVMALPDAALEQQADRLLAGITLGLDLA
                     AAPLVAAGLQLYFTHLVAATGAARPQAFQVPPDATRCPCCASLATASITRVGGQQDGQ
                     RYLHCALCSAQWHMNRIQCTHCLATQGIHYQSLQALADDKPSAQRAAVEAETCDGCHH
                     YLKTLHVARDLHVEPLADDLATLTLDLLVSEAGFERHGVNLLLLFGDGDAQAGGD"
     gene            complement(3325..3969)
                     /locus_tag="PK518_11135"
     CDS             complement(3325..3969)
                     /locus_tag="PK518_11135"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HRM49513.1"
                     /translation="MKKLQLQRYNAGQRSNHWATVTCFVLAAVSGFALFHPALFWLTA
                     LVGGPQWARILHPYLGLAMFVLFLGMFFMFVGANLWRDEDSAWLESAGKMVSEGNQAV
                     MPPVGKYNGGQKLIFWVFTLCLLVLLASGLLFWQAWFASSVPIWLQRIAVLAHAVSAF
                     VLILAVIVHAYAALWVKGTLGAMVRGRVSVGWAKHHHPLWYREQVQQQTPKAHK"
     gene            complement(3966..4877)
                     /gene="fdxH"
                     /locus_tag="PK518_11140"
     CDS             complement(3966..4877)
                     /gene="fdxH"
                     /locus_tag="PK518_11140"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HRM49514.1"
                     /translation="MSSLQSQDVVARSATTTPPPQARAHVPEVAKLIDVSKCIGCKAC
                     QVACMEWNDLRDEVGSNIGVYDNPSDLTPQSWTVMRFFEVEPEKDKLEWLIRKDGCMH
                     CEDPGCLKACPAPGAIVKYANGIVDFISEHCIGCGYCIKGCPFNVPRISQQDNKAYKC
                     TLCSDRVGVGLEPACVKTCPTGAITFGTKEDMVASGQERAAELKERGWSQAGLYNPAG
                     VGGTHVMYVLKHADKPELTQLPKDPSISPWVSVWKGWTKPAALAVMAGAAFTGWFHYL
                     RKGPMEVPEEDEEYADSPDSRTQGEQS"
     gene            complement(4880..7960)
                     /gene="fdnG"
                     /locus_tag="PK518_11145"
     CDS             complement(4880..7960)
                     /gene="fdnG"
                     /locus_tag="PK518_11145"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011193342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7373..7375),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HRM49515.1"
                     /translation="MDMHRRQFFRVSAAGLVGSSLVAMGFSPAAALAETRAFKLASAT
                     VTRSICPYCSVSCGMLIYKLGDGAKNAKNHVIHVEGDPDNPVSRGSLCPKGAGVMDMI
                     NSPGRVKYPSVREAGSKEWKRISWDEALTRVAKHMKADRDANFQTKNDAGTTVNRWTT
                     TGFLMCSSSSNESSYLTVKVARGLGMVGIDTQARVUHAPTVSSLGPTFGRGAMTNSWT
                     DIKNADLIIVMGGNAAEAHPVGFKWVVEAMQKRKARLISVDPRFNRTSALADLYAPIR
                     NGTDIAFLGGVINWLLSNDKIHHDYVKFNTDATFLTKPGFDLKDGIFSGYDAAKRKYD
                     KSTWGYQMGEDGYVKVDETLQDPLCVYQQLKKHYSRYTPEMVSKICGTPQDKFLKICE
                     MLGEMSAPDKTSTICYALGWTQHTVGSQNIRTMAMIQLLLGNMGRPGGGVNALRGHSN
                     VQGVTDMNAYAEVFSGYLSAPTDNDDTYEKYLKRCTPKPMRPNQFNYWSNFPRFFNSL
                     MKSYYGKAATPESNFCYDWVPKLAGGFDVLHMFELMHQGKMNGFLAQGFNPLATVPNK
                     NKLSAALSKLKYLVIIDPLESETGDFWQNYGEFNDVKPEAIQTEVFRLPSTCFAEEEG
                     SLTNSSRVAIWKERAVDPPGEAKTDSEIMALLFMKLRSMYAKDGGAFPDAVLNLAWDY
                     AQPLEPSASELLREINGKALADVLAPADPANPGAPRAVLVKAGQQVPGFAMLRDDGST
                     ACGNWLYSGCWSEAGNLTARRGLDDPSGLGVFQHFGFAWPANRRILYNRASADKDGKP
                     WAPNKKYEYWNGKAWTGPDVPDMRPNAAPEENVGSFIMNPEGVARLHAIGMAEGPFPE
                     HYEPFETPIGVNPLHPDNPLAISNPAARVFKGDLEAFGKKEEFPYSATTYRLTEHFHT
                     WTKHARLNAITQPEAFVEISEELAKEKGLKHGDRVKVSSNRGHIITKAVVTKRIKPFA
                     VNGQQVHLVGIPFHWGFKGQTKNGYIANTLTPFVGDANSQTPESKAFLVNIEKV"
     gene            complement(8107..9312)
                     /locus_tag="PK518_11150"
     CDS             complement(8107..9312)
                     /locus_tag="PK518_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49516.1"
                     /translation="MTGPERRSSMGLAAIFAMRMLGLFLVLPVFMLEARKYPGGDDPA
                     LVGLAMGMYGLTQAFFQLPLGMASDRFGRKRVIVLGLAVFAAGSLVAALADSVMGLLV
                     GRALQGAGAVSAAVTALLADQTRDAVRTKAMALVGISIGLMFAIALVLAPLLTAHIGL
                     SGLFTLTFALALAGVALIVWVVPPETRHHADLPQGRLADVWRHADLRRLNLGVFALHT
                     IQMAMWVAVPALLVQAGLPKDLHWHVYLPALVLSVVAMGGLFSMERRGRLRAALLLSI
                     GLIALVQAALGGLVAGGVAPGVMLLGGLLFVFFCGFNMLEATQPSLVSRMAPANLRGA
                     ALGAYNTLQSLGLFAGGALGGALAKWAGTGGLFAATALLALAWLVITWPLQPVGRAGA
                     GDAHLRPST"
     gene            9534..>9811
                     /locus_tag="PK518_11155"
     CDS             9534..>9811
                     /locus_tag="PK518_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49517.1"
                     /translation="MAQVLRAPCISIRGARTHNLKNIDLDIPRNRLVVITGLSGSGKS
                     SLAFDTLYAEGQRRYVESLSAYARQFLGRLDKPDVDLIEGLSPAISIE"
BASE COUNT         1474 a         3238 c         3475 g         1624 t
ORIGIN      
        1 gccggccagg gccacggcac agcgctggcc ggcatgggcc tgctgcacgc cctggttttg
       61 cgcgtgcagg ctgcgcacgc gcgcgcgcag tggcgcgccg ccgccgctgg tgctgatggc
      121 cagctcgtcg cccacacgca cgctgccggc gtggatggtg ccggtgacca ccgttcccgt
      181 gccatccagt gtgaaggcgc ggtctatggc caggcgcagg ccctgcgcgg cgccggccgc
      241 cgctgcctgt ggctgcccct gggcggcgtc gaacagcagc tggcgcaacg gcgcaatgcc
      301 gctgccggtc tgcgccgata cgttgacaac gggtgcgccg gccagcgatg tgccctgcag
      361 cagtcgcgcc atctcagcgc gcagggcctg cagcctgtcc gggctggcgc ggtcggtctt
      421 ggtgatgacc acggcgccgc gcgctatgcc cagcagcgac agcacggcca ggtgctcgtg
      481 cgtctgcggc atggggccgt catcggcggc caccagcagc agcgcgtaat cgatgccgct
      541 ggcgccggcg accatggtgt gcaccaggcg ttcgtggccg ggcacgtcga tgaagccgat
      601 gcgcccgccc gcgggcgtgt ccaggtaggc gtagcccagc tctatgctga tgccgcgctt
      661 tttctcctcg ggcaggcggt cggtgtccac gccggtgagg gcgcgcacca gcgtggtctt
      721 gccgtggtcg atatggcctg cggtgcctac aatcattttg atagctgtca gcgcttgtca
      781 gataagcgca aaaggccaat ttgacctatg aatacttggg cgtcttccag gcagcgcatg
      841 tccagcagca ggcggtcgtc ctgcacccgg cctatgacgg gcagcggcag agcgcgcagc
      901 gcctcggcca gctgcgccag ggcccggccc acgcccttgg cgtcaacggg ggcgatcgcc
      961 aggccgctgg acgccaggcg ctccacgggc agcgagccgg agccgatctg gccctgcaac
     1021 gccaccggct gcacctggta gcgcggcgcc agggcggcct gcagcgcggg cagcacggct
     1081 tgcgccgtgg ccgttatgtc ggccagcggg cgcgtgagca ggcgcagcgt gggcaggtcc
     1141 tgcgccaggc gctcggggcg caggtacagg cgcagcgtgg cctccagcgc ggccaggggc
     1201 agcttgctca tgcgcagcgc gcgcttcatg gggtgcttgc ggatgcgcgt gagcaagtcc
     1261 ttgcgcccga cgatcagccc ggcctgcggg ccgcccaaca gcttgtcgcc gctgaaggtg
     1321 acaaggtcac agccggcggc cagcatctgc tgcggcgtgg gctcgggcgg caggccgtag
     1381 gcgctcatgt cgatcagcgc accgctgccc aggtcggtgg ccaggggcag gccgcgggcg
     1441 tgggcgatgg gcgccagccg tgcctcgtcc acggcactgg tgaagccctg cacggcgtag
     1501 ttgctggtgt gcaccttcat cagcagcgcg gtgtgctcgc cgatggcgcg ctcgtagtcc
     1561 tgtgcgtgcg tgcggttggt ggtgcccacc tcgaccagcc gggcgccggc gctggccatc
     1621 acatcgggca tgcgaaaggc gccgccaatt tccaccagtt cgccacgcga gacgatgacc
     1681 tccttgcccg gcgccagggc cgccagcgtg agcagcacgg cggcggcgtt gttgttcacc
     1741 acggtggcgg cctcggcgcc ggtcagctcg cacagcaggc cctcgacgat gctgtcgcgg
     1801 tcgccacggc taccgctggc caggtcgtac tccaggttgt tcgggctggc catcatggcc
     1861 tggatatgcg ccagcgccgc ctgcggcagc agggcgcgcc ccaggttggt gtggatcacc
     1921 gtgccggtga ggttgagcac ggcgcgcatc gccggctgca ggcgccgctg caggcgctcg
     1981 gccagggcgc cggccagggc gtcggcgccg atctggccga gtgccaggtc gccggccagg
     2041 gcctgcgcgc gcagggtggc cagcagcgcg cgcgcctcgt tggcgaccag ggtgtggcca
     2101 tgggcatcga gcagcgccgc gcaggcgggc aggcgcagca gcctgtcgag cgcgggcagg
     2161 ttctgtggtt gtgcggcggg tggggggctg gcgggggccg gggaaccgtg aaccacggat
     2221 tcttcgggca gtttcatcag tcgcccccgg cctgcgcgtc gccgtcgcca aagagcagca
     2281 ggaggttgac gccgtggcgc tcgaagccgg cctcggacac cagcagatcc agcgtcagcg
     2341 tggccaggtc gtcggccagc ggctccacat gcaggtcgcg cgccacatgc agggtcttga
     2401 ggtagtggtg gcagccatcg caggtctcgg cctcgacggc ggcgcgctgg gccgatggct
     2461 tgtcatcggc cagcgcctgc agcgactggt agtgaatgcc ctgcgtggcc aggcaatgcg
     2521 tgcactggat gcggttcatg tgccactggg cgctgcacag cgcgcagtgc aggtagcgct
     2581 ggccgtcctg ctggccgccc acgcgcgtga tgctggccgt tgccaggctg gcgcagcagg
     2641 ggcagcgggt ggcgtccggc ggcacctgga aggcctgggg ccgggcggcc ccggtggcgg
     2701 ccaccaggtg cgtgaagtac agctgcaggc cggcggcgac gagcggcgct gcggccagat
     2761 ccagccccag cgtgatgccg gcgagcaggc gatcggcctg ctgctccagc gccgcgtcgg
     2821 gcagggccat gacggcctgc acgccggcgc gcgccgggct gtcaccgggc agcttgtcca
     2881 gcacctgccg cagcagggcg cgcagctcgg tgcgccactg cgcggcgcgc ggccagtgtt
     2941 cggcggccag caggggctcg ccggactcgc cggcggcggc ggcctgcgcg gcatcgggca
     3001 gggttacctc cgggtactgc tgcaggcgcg cgtgctgggc ctcgcagacc acggccatga
     3061 gcatcaggta gtcgcgcatg gcgtggccgg cggccagctg gcgcaggcgc agggcgcgct
     3121 cggcaaacag cttgccgggg tcgggcagca gcagcggggg gacttccagg cccgagcgct
     3181 gctttatgtc ctcggggctg ttgatctggc gatgggtggg gctggagttc aaggctgcaa
     3241 caggatcagg gtcagaaaaa aggctgctgg cagtgtgcac cactgcgcag cagcccgggg
     3301 cgggcccacg atgggggaat acgcctactt gtgcgccttg ggcgtttgct gctgcacctg
     3361 ctcgcggtac cacagcgggt ggtggtgctt ggcccagccc acgctgacgc ggccgcgcac
     3421 catggctccc agcgtgccct tgacccacag ggccgcgtag gcatgcacga tcacggccag
     3481 tatcagcacg aaggccgaca cggcgtgcgc cagcacggcg atgcgctgca gccagatcgg
     3541 cacgctggag gcaaaccagg cctgccagaa cagcaggccg ctggccagca gcaccagcag
     3601 acagagcgtg aacacccaga agatcagctt ttgcccgccg ttgtacttgc ccaccggcgg
     3661 catcaccgcc tggttgcctt cggagaccat cttgccggcg gactccagcc aggccgagtc
     3721 ctcgtcacgc cacaggttgg cgcccacgaa catgaagaac atgcccagga acagcacaaa
     3781 catggccagg cccagatagg ggtgcagaat gcgcgcccac tgtggcccgc cgaccagcgc
     3841 cgtgagccag aacagcgccg ggtgaaacag cgcgaagccg gatacggcgg ccagcacgaa
     3901 gcaggtcacc gtcgcccagt ggttggagcg ctggccggcg ttgtagcgtt gcagctggag
     3961 ctttttcatg actgctctcc ttgcgtcctg gagtcagggg agtcggcata ctcctcgtcc
     4021 tcctcgggta cttccatcgg gcccttgcgc aggtagtgga accagccggt gaaggccgcg
     4081 ccggccatca cggccagcgc cgccggcttg gtccagccct tccagaccga cacccagggg
     4141 ctgatgctgg gatccttggg cagctgcgtc agctcgggct tgtcggcatg cttgagcaca
     4201 tacatgacat gcgtgccgcc cacgccggcc gggttgtaca gcccggcctg gctccagccg
     4261 cgctccttga gctcggcggc gcgctcctgg ccgctggcca ccatgtcctc tttggtgccg
     4321 aaggtgatgg cgcccgtggg gcaggtcttc acgcaggccg gctccaggcc cacgcccacg
     4381 cggtccgagc acagggtgca cttgtaggct ttgttgtcct gctggctgat gcgcggcacg
     4441 ttgaacgggc agcccttgat gcagtagccg cagccaatgc aatgctcgct gatgaagtcc
     4501 acgatgccgt tggcgtactt gacgatggcg ccgggcgcgg ggcaggcctt gaggcagccc
     4561 gggtcttcgc agtgcatgca gccgtccttg cggatcagcc attcgagctt gtccttctcg
     4621 ggctcgacct cgaagaagcg catcaccgtc caggactggg gcgtgaggtc gctggggttg
     4681 tcgtagacgc cgatgttgct gccgacctcg tcgcgcaggt cgttccactc catgcatgcc
     4741 acctggcagg ccttgcagcc tatgcacttg gagacgtcga tgagcttggc gacctcgggc
     4801 acatgggcac gtgcctgtgg cggcggggtg gtggtggccg agcgggcgac cacgtcttgt
     4861 gattgcagac tcgacatggc tacacctttt caatattgac gaggaaggcc ttggactccg
     4921 gcgtctgcga gttggcgtcg ccgacgaagg gggtcagggt attggcgatg tagccgttct
     4981 tggtctggcc cttgaagccc cagtggaacg gaatgcccac cagatgcacc tgctggccgt
     5041 tgaccgcgaa cggtttgatg cgcttggtga ccaccgcctt ggtgatgatg tggccacggt
     5101 tgctgctcac cttgacccgg tcgccgtgct tgagcccctt ctccttggcc agctcctcgc
     5161 tgatctccac gaaggcctcg ggctgcgtga tggcgttgag ccgcgcatgc ttggtccagg
     5221 tgtggaaatg ctcggtcagg cggtaggtgg tggccgaata cgggaattcc tccttcttgc
     5281 caaaggcctc caggtcgccc ttgaacaccc gggccgcggg gttgctgatg gccagggggt
     5341 tgtccgggtg cagggggttg acgccaatcg gcgtctcgaa cggctcgtaa tgctcgggga
     5401 agggaccttc ggccatgccg atggcgtgca gtcgcgccac gccctcgggg ttcatgatga
     5461 aggagcccac gttctcttcg ggcgccgcat tgggccgcat gtcgggcacg tctgggccgg
     5521 tccaggcctt gccgttccag tactcgtact tcttgttcgg cgcccagggc ttgccgtcct
     5581 tgtccgcgct ggcgcggttg taaaggatgc ggcggttggc cggccaggcg aagccgaaat
     5641 gctggaacac gcccaggccc gaggggtcgt ccaggccgcg gcgcgcggtc aggttgcccg
     5701 cctcgctcca gcagcccgag tagagccagt tgccgcaggc ggtggagcca tcgtcgcgca
     5761 gcatggcaaa gcccggcacc tgctgcccgg ccttgaccag caccgcgcgc ggcgcgcccg
     5821 ggttggccgg gtcggcgggc gccagcacgt cggccagggc cttgccgttg atctcgcgca
     5881 gcagctcgct ggccgagggc tccagcggct gggcgtagtc ccacgccagg ttcagaaccg
     5941 cgtcggggaa ggcgccgccg tccttggcgt acatggagcg cagcttcatg aagagcagcg
     6001 ccatgatttc cgagtcggtc ttggcctcgc ccgggggatc gaccgcacgc tccttccaga
     6061 tcgccacgcg gctggagttg gtaagactgc cctcttcctc ggcaaagcag gtgctgggca
     6121 ggcggaagac ctcggtctgt atggcctcgg gcttgacgtc gttgaactcg ccgtagttct
     6181 gccagaagtc gcccgtctcc gactccagcg ggtcgatgat caccaggtac ttcagcttgg
     6241 acagcgccgc cgagagcttg ttcttgttcg gcaccgtggc cagcgggttg aatccctgcg
     6301 ccagaaaacc gttcatcttg ccctggtgca tcagctcgaa catgtgcagc acgtcgaagc
     6361 cgccggccag cttgggcacc cagtcgtagc agaagttgct ctccggcgtg gccgccttgc
     6421 cgtagtagga cttcatcagg ctgttgaaga acctggggaa gttgctccag tagttgaact
     6481 ggttgggccg catgggcttg ggcgtgcagc gcttcaggta cttttcgtac gtgtcgtcgt
     6541 tgtcggtggg cgcgctcagg tagccggaga acacctcggc gtaggcgttc atgtcggtca
     6601 cgccctgcac gttggagtgg ccgcgcagcg cattcacgcc gccgcccggg cgccccatgt
     6661 tgcctagcag cagctggatc atcgccatgg tgcggatgtt ctgactgccc acggtgtgct
     6721 gcgtccagcc cagggcgtag cagatggtgc tggtcttgtc gggcgccgac atctcgccga
     6781 gcatctcgca gatcttcagg aacttgtcct gcggcgtgcc gcagatcttg ctgaccatct
     6841 cgggcgtgta gcggctgtag tgctttttca gctgctggta gacgcacagc ggatcctgca
     6901 gtgtctcatc gaccttgaca tagccgtcct cgcccatctg gtagccccag gtggacttgt
     6961 cgtacttgcg cttggccgcg tcgtagccgc tgaaaatgcc gtccttgagg tcaaagccgg
     7021 gcttggtcag gaaggtggcg tcggtgttga acttgacgta gtcgtggtgg atcttgtcgt
     7081 tgctgagcag ccagttgatg acgccgccca ggaaggcgat gtcggtgccg tttctgatcg
     7141 gcgcgtacag gtcggccagc gccgacgtgc ggttgaagcg cgggtcgacc gagatcagcc
     7201 tggccttgcg cttctgcatg gcctcgacca cccacttgaa gcccaccggg tgcgcttcgg
     7261 cggcgttgcc gcccatcact atgatcagat cggcattttt aatgtcggtc catgaattgg
     7321 tcatcgctcc gcgaccgaaa gtcgggccca gactggacac cgtcggtgcg tgtcagacgc
     7381 gtgcttgtgt atcgattcca accatgccaa ggccgcgcgc gaccttgacg gtgaggtaac
     7441 tcgattcatt ggaggacgag ctgcacatca ggaaaccggt ggtcgtccag cggttcaccg
     7501 tggtgccggc atcgttcttg gtctggaagt tggcgtcgcg atcggccttc atgtgcttgg
     7561 ccacgcgcgt cagggcctcg tcccagctga tgcgcttcca ctccttgctg cccgcttcgc
     7621 gcaccgatgg gtacttgacg cggccggggg agttgatcat gtccatcacg cccgcgccct
     7681 tggggcacag cgagccacgg ctgaccgggt tgtccggatc gccctcgacg tggatcacat
     7741 ggttcttggc gtttttcgcc ccatcgccca gcttgtagat cagcatgccg cagctgaccg
     7801 agcagtaggg gcagatgctg cgcgtgacgg tggcgctggc gagcttgaag gcccgggtct
     7861 cggcgagtgc ggcggcgggt gagaacccca tggccacaag actcgacccg accagccccg
     7921 ccgcgctgac gcggaagaac tgtctgcggt gcatgtccat gacttgtctc ctgaatggtt
     7981 gagcgccctg gtggggatcg cgcgctgccc cggcggcagg gggctgattc agtgtaggca
     8041 agccgaatgt tgcgccggaa ccacgttaac ccttaagggt cgcgtgcggc ggctggctcc
     8101 cggccgtcag gtggagggcc gcaaatgggc atcgcccgcg ccggcccgtc ccaccggctg
     8161 caggggccag gtgatgacca gccaggccag ggccagcaac gccgtggcgg caaacagccc
     8221 acccgtaccg gcccatttgg ccagggcgcc gcccagggcg ccgccggcaa acaggcccag
     8281 ggactgcagc gtgttgtacg cgcccagggc cgcgccgcgc agattggccg gcgccatgcg
     8341 cgagaccagg ctgggctgcg tggcctccag catgttgaag ccgcagaaga acacgaacag
     8401 caggccgccc agcagcatca ccccgggtgc cacgccgccg gccaccaggc cgcccagggc
     8461 cgcctgcacc agggcaatca ggccaatcga cagcagcagg gccgcacgca ggcgcccgcg
     8521 ccgctccatg gagaacagcc cgcccatggc cacgaccgac agcaccagcg ccggcaggta
     8581 gacatgccag tgcaagtcct tcggcaaccc cgcctgcacc agcagggcgg gcacggccac
     8641 ccacatggcc atttgaatgg tgtgcaacgc aaacacgccc aggttcaggc gccgcaggtc
     8701 ggcatggcgc cagacatcgg ccagccggcc ctgcggcagg tcggcgtggt ggcgcgtctc
     8761 gggcggcacc acccagacga tcagcgccac gccggccagg gccagggcaa aggtcagcgt
     8821 gaacaggccg gacaggccga tatgggccgt cagcagcggc gccagcacca gggcgatggc
     8881 gaacatcaag cctatgctga tgcccaccag ggccatggcc ttggtgcgca cggcgtcgcg
     8941 cgtctgatcc gccagcaggg ccgtgacggc ggccgacacc gcgcccgcgc cctgcagcgc
     9001 ccggcccacg agcaggccca tgaccgaatc ggccagcgcc gccaccaggc tgccggcggc
     9061 gaataccgcc aggcccagga cgatgacgcg cttgcggccg aagcggtcgg acgccatgcc
     9121 cagaggcagc tggaagaagg cctgggtcag gccatacatg cccatggcca ggccgaccag
     9181 ggccggatcg tcgccgccgg ggtatttgcg cgcctccagc atgaacaccg gcagcaccag
     9241 gaacaggccc agcatgcgca tggcaaagat ggccgccagc cccatgctgg agcggcgctc
     9301 ggggccggtc atgcgtgtgg cgggggcagc gggggcggag gtggaaggca agatggtttc
     9361 tgacacggcg ggtgttcggt ggtcaggcaa aacccgtatt ctcgcccagg gtggttttgc
     9421 cgtgccgcgg gtgggggcgg cgcggtgcgc gtggctatca tggcgggttc cccagcgccg
     9481 ccccgatcgt gacctaccct gcaccctcct ccgatgattc cgacggccgc ccgctggccc
     9541 aggtgctgcg cgcgccctgc atcagcatcc gcggcgcacg cacgcacaac ctgaagaaca
     9601 tcgacctgga cataccgcgc aaccggctgg tcgtcatcac cgggctgtcg ggctcgggca
     9661 agtccagcct ggcgtttgac accctttatg ccgagggcca gcgccgctat gtggaaagcc
     9721 tctcggccta tgcgcgccag tttctcggac ggctggacaa gcccgatgtc gatctgatcg
     9781 agggcctgtc gccggcgatc agcatcgagc a
//
LOCUS       DAOMTQ010000115         9710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1401_length_9710_cov_24.137442, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000115 DAOMTQ010000000
VERSION     DAOMTQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_9710_cov_24.137442"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PK518_11160"
     CDS             complement(<1..815)
                     /locus_tag="PK518_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRM49518.1"
                     /translation="MKAARFHARGDIRIEDIPEPTVAPGTVGIDVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVTDIQVGQHVVVEPYIIAD
                     DVPTGPGERYHLSKNMNFIGLGGRGGGLSEKIAVKRRWVHPISNAIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGGGPIGLLLAAVLKAKGLKVIITELSAKRKEKARESGV
                     ADHILDPSQVDVVAEVMKITGDQGADVAFECTSVNKVLDQLVAATKPGGVVVIV"
     gene            1154..1561
                     /locus_tag="PK518_11165"
     CDS             1154..1561
                     /locus_tag="PK518_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRM49519.1"
                     /translation="MFANLTPFLIHWATLALGLWVCSHVFKGIRFDGVGALVISALLL
                     GLANAVVRPLLVFLTLPLTLITFGLFLLVINALVLMLVAKLVNGFYLSGFWTAFWASI
                     FMSLLSLVLGAFVLGGTPEFIIETGPRAGSTWL"
     gene            1787..2605
                     /locus_tag="PK518_11170"
     CDS             1787..2605
                     /locus_tag="PK518_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRM49520.1"
                     /translation="MELQALLAQPIALPSLPRAVALLMSELAQAEPSLRRLNQFFGTD
                     PALAARLLQEANASAFGAQQQISGIPEALALLGVAQLRTLVRAAPLGASSRSVPGVNL
                     QLFWRYSLNTAKLARSFAGLVQQSQIVAYTAGLLHGVGELALHLADPERMQSINSLMP
                     PLDPRRAKLEQRLLGYCYAQVSAELARRWQLPLVVVDALRHHHAPFDNNDYEPLAGVL
                     HLAAWRARAREAGLSERELAVSFPGKVGLTLGLDIDMVLQQDPIDWKARPDAGD"
     gene            2648..3520
                     /locus_tag="PK518_11175"
     CDS             2648..3520
                     /locus_tag="PK518_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HRM49521.1"
                     /translation="MSMKQTLVALAAGITWALGAQAQCLSDGDVGVMASHYASKTPAP
                     NFPALSDADGACTRAKFNALLASSFGKVVGYKAGLTNPAVQKRFNTDKPVWGKLYEGM
                     IEQSGASVPAAFGARPLFEADMLVRVKDAAINQASTPYEVLQHIDQVIPFIELPDLVV
                     QAPAQLNGPGVAAINVGARLGVAGQPIAVPATRGERYALLDALERMQVQLTNNQGQLL
                     ASGKGSDILGQPLNAVVWIAQALQKDGITLQPGDLISLGSFSPLLPPRPGLSVTASYV
                     GLPGAQPVQVHFKQ"
     gene            3517..4494
                     /locus_tag="PK518_11180"
     CDS             3517..4494
                     /locus_tag="PK518_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM49522.1"
                     /translation="MTNTDLAAQTHARLTPQMRSVVDRLARAGRPALHTLSPAQARIA
                     YEAGAGVLEVPRAELPRVEDLHVPSRDGVQLPARLYAPGTAAALPALLYLHGGGFTIG
                     SVATHDVLCRELARLGRCMVLSLDYRLAPEHAFPTASNDAWDALAWLAANAARLGADP
                     GRLAVGGDSAGGTLAAVNAILARDAGLPLALQLLIYPGTTAHQDTLSHTEFAHGPILD
                     RAAIGWFFDQYIPSRAEREDWRFAPLLAPDVDGVAPAWIGLAECDPLVDEGVEYGDKL
                     RAAGVAVDLEIYRGVTHEFIKMGRAIAEARRAHQDAARALRAAFGIDGG"
     gene            4499..5359
                     /locus_tag="PK518_11185"
     CDS             4499..5359
                     /locus_tag="PK518_11185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HRM49523.1"
                     /translation="MQRQDFRCFHRLRVRWAEVDIQKIVFNAHYLTYADCAMTDYWRA
                     LALPYESGMQALGGEVYLKKASVEYHASARLDDLLDVGMRCARVGHTSLLFECAMFAG
                     DRLLATVELVYVFADPATQTKRPVPPALRAMIEHYEAGGEMVELRVGDWATLGADATA
                     LRMAVFVQEQGIDPAIELDAHDPTAVHAVAYNRLAQPVATGRLLYEAPGDARIGRMAV
                     DRVVRGQRWGRAVLDALVRAAQARGDAQVTLHAQCSAEGFYRRAGFVPVGAVFEEAGI
                     AHITMRRTLA"
     gene            complement(5444..5932)
                     /locus_tag="PK518_11190"
     CDS             complement(5444..5932)
                     /locus_tag="PK518_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRM49524.1"
                     /translation="MQAKTFALLATAVIAGAANAQAVRSEKNMSLDLASQIATHSVAA
                     CAANGYAVTATVVDRAGQVRAQLRADNAGPHTLEASRLKAYTSASAKNATSAIMETSQ
                     KNPGAANLGQIPGYLLLGGGLPVKAGNEVIGAVGIGGAPGGHLDEQCAKAALDQVQEL
                     LK"
     gene            complement(6052..6696)
                     /locus_tag="PK518_11195"
     CDS             complement(6052..6696)
                     /locus_tag="PK518_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM49525.1"
                     /translation="MIAANADLSPLIHLVDDDDAVRNSLALLISTIGLRVQTWADPLA
                     FSTQFDRQGIGAVVLDVRMPGIGGLALLAQLVEQGVDHPIVLLTGHGTVDMCRRAFKA
                     GAAEFLEKPVDDELLLETLQAAVRQHVRARQQRQADRDAQQRFAELSPRERQVLALIV
                     QGLSNKDMARVLELSPRTVEVHRANLFAKLQVPSLAHLVRRYASLAEMDAPPAA"
     gene            complement(6693..8162)
                     /locus_tag="PK518_11200"
     CDS             complement(6693..8162)
                     /locus_tag="PK518_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM49526.1"
                     /translation="MNGLRRMAPWWLAWLVLGAAGCVWLAQQRLQQLHDSFDTDARIA
                     HRVLSQRMVQHEAILATLVLLQPESRATEPAPWSALTRPYPQVREVLRHDHGAPAWPA
                     GWPPGMDAALAQSRASGHAVLAPSNLTGGQLYLVQAGTPASFALRLDLRTTALADDWP
                     LSPGSPVHAWLALDGQRYTIQGAAENTARWQWQSAKTLAATSQPLVLHMQQAVRAAML
                     PWGSMLGWLAGSAALLTALRQLLRQRVARRRAEQLLQLGQVARLNTLGELAAGMAHEL
                     NQPLTAVLASSQAAQRLLAEDAPDMPLLRQALAQVVAQARRASDVVTRLRRLVERPDA
                     AMQSSAIDLPQAVIDALHLLEPQLSAHKVHLHPQFAPGLPAARADAVALQQILHNLLT
                     NALQAMDTTPVGERQLWITLTHQGAQLLLTVRDSGPGIAPDMRPRLFTPFATGRDRGL
                     GLGLTLSQSLAEAMGGSLQLHDAGPGACFALRLPVHNTR"
     gene            complement(8185..9375)
                     /locus_tag="PK518_11205"
     CDS             complement(8185..9375)
                     /locus_tag="PK518_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49527.1"
                     /translation="MAERPRIALAWLATVVLVTLNLRPFLTAIGPLTPTIQAHTGLGL
                     QALAWLTLLPMALMGAGTWLAPALLPRLGARPTLALALTLLALGCALRLVPAWLLATA
                     ALCGVGVALVQGTLPGLIKLHTPRHVAPMMGVYSASLMGGGALGAQLSPLALQWGLGW
                     SGALALWTLPVLAALALAWWQLGRMATPAAGATQRGASAWLLRRPRTWYLMLAFGLMN
                     GGYASMIAWLAPHYQELGWSAAQSGTLVALLSIAQAAAALLLPVLAARGGPDRRPWLW
                     FTLGCQALGFALLAMAPLAAPLATAIVLGVGLGGCFTFFMLVALDHLRAPLQAGALNA
                     LMQGGGFLLSALPPWLMARLHQGGGGFSGGWWLQCAMALLVAVLVARLAPTQYERVMQ
                     PPVP"
     gene            complement(9400..>9710)
                     /locus_tag="PK518_11210"
     CDS             complement(9400..>9710)
                     /locus_tag="PK518_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HRM49528.1"
                     /translation="FKKGCYPGQEVVARSQFRGTLKRRAYLAHAPAPVAVGAEVFAQD
                     DAEQPVGLVVQAAAAPGGGVDALVSLQIAATAGALQVGAAQGVPLTLLPLPYALLEDI
                     "
BASE COUNT         1448 a         3327 c         3414 g         1521 t
ORIGIN      
        1 acgatcacca ccacgccgcc cggtttggtg gcggccacca gttggtcgag caccttgttg
       61 acgctggtgc attcaaaggc cacgtcggcg ccctggtcgc cggtgatctt catcacctcg
      121 gccaccacgt ccacctggga cgggtcgagg atgtgatcgg ccacgccgga ctcgcgcgcc
      181 ttctccttcc gcttggcgct cagctcggtg atgatgacct tcaggccctt ggccttcagc
      241 acggccgcca gcagcagccc gatggggccg ccgccgccca ccagcgccac gtcgccggcc
      301 ttggcgccgc tgcgcacata ggcgtggtgg cctactgaca ggggctcgat cagggccgcc
      361 tggtccaggg gaatggcgtt ggagatcggg tgcacccagc ggcgcttgac ggcaatcttt
      421 tcagacaggc cgccgccgcg accgcccagg ccgatgaagt tcatgttctt ggacaggtgg
      481 tagcgctcgc ccggcccggt gggcacgtcg tcggcaatga tgtagggctc gaccaccaca
      541 tgctggccca cctggatgtc ggtcacgcct tcgccgacgg catagaccac gcccgagaac
      601 tcatggccca tggtgacggg cgcggactcg cccgatatgg ggtgcgggtg gccgcagggc
      661 ggaatgaaga tcggcccctc catgaactcg tgcaggtcgg tgccgcagat gccgcaccag
      721 gcgacgtcga tgccgacggt gccgggggcc acggtaggct ctggaatgtc ttcgatgcgg
      781 atgtcgccgc gggcgtggaa gcgtgctgct ttcatggttc aagctcctgt gctgggtgga
      841 agattggggg gctcggcggc ttgcgccacg atgcactgcc tgggcgctgc cgagtgagag
      901 aggccaggcg catggcccga gagttcacgg gccgcgtggg cgcgccaagc gatgggacat
      961 tgtcacgccg cagggctctt ctgtccatgc atggcgatgg gccggtcacc gccgtggtgt
     1021 gtcgtcctct gcaactgttt tgatagctgg atgcgcatgc actgcaagcg tgccgagcga
     1081 attcgtcaag tatttgcaat gcacctgcgt gcatagaatg cacgcatcat tcacctcgcc
     1141 gcgcggcgcc gccatgtttg ccaacctgac tccattcctg atccattggg ccacgctggc
     1201 gctgggcctg tgggtctgca gccatgtgtt caaaggcata cgctttgatg gcgttggcgc
     1261 gttggtcatc tcggcgctgc tgctcggcct ggccaacgcg gtcgtgcggc cgctgctggt
     1321 gttcttgacg ctgccgctga cgctgatcac cttcggcctc ttcctgctgg tgatcaacgc
     1381 gctggtgctg atgctggtgg caaagctggt gaacggcttt tatctgagcg gcttctggac
     1441 ggccttctgg gccagcatct tcatgtcgct gctcagcctg gtgctgggcg ccttcgtgct
     1501 gggcggcacg ccagagttca tcatcgaaac cggccccagg gccggatcga cctggttgtg
     1561 aggaatcagt ggtgcctgct gccgccataa tggccgtttc tttttgcgtc ccacctgaga
     1621 ggctgagagg aaattccccg tgcccgcttt acacgccaaa acacccccac tcttcgttgc
     1681 aaatactcgc catagcccga gctatggctg cgttttgcgt ctcgattggc ggcgttttga
     1741 cgcgtccttc gggcacggcc aatttactct cagcctctga cctgtcatgg agcttcaagc
     1801 cttgctggcg cagcccatcg cgctgcccag cctgccacgc gcggtggcgc tgttgatgag
     1861 cgagctggcc caggccgagc ccagcctgcg gcgcctaaac cagttcttcg gcaccgatcc
     1921 ggccctggcc gcacgcctgc tgcaagaggc taacgcgagc gcctttggcg cgcagcagca
     1981 gatcagcggc attcccgagg cgctggccct gctgggcgtg gcgcagctgc gcacgctggt
     2041 gcgcgcggcg ccgctgggcg cctcctcgcg ctcggtgccg ggcgtcaacc tgcagctgtt
     2101 ctggcgctac agcctgaata ccgccaagct ggcgcgatcc tttgccggcc tggtgcagca
     2161 aagccagatc gtggcctata ccgccgggct gctgcatggc gtgggcgagc tggcgctgca
     2221 cctggcagac ccggagcgca tgcagtccat caacagcctg atgccgccgc tggatccgcg
     2281 ccgcgccaag ctggaacagc gcctgctggg ctactgctac gcccaggtga gtgccgagct
     2341 ggcccgccgc tggcagctgc ccctggtggt ggtggacgcc ctgcgccacc accacgcacc
     2401 gtttgacaac aacgactacg aacccctggc cggcgtgttg catctggccg cctggcgcgc
     2461 ccgggcgcgc gaggcgggcc tgagcgagcg cgagctggcg gtgtcctttc ccggcaaggt
     2521 cggcctgacc ctgggcctgg acatcgacat ggtgctgcag caggatccca tcgactggaa
     2581 ggctcgcccg gacgccggcg actagatctg ccaccatcgg ccgcataccc acaaacagga
     2641 gactggcatg agcatgaagc agaccctcgt cgcgctggcg gcgggcatca cctgggcttt
     2701 gggcgcgcag gcgcaatgtc tgagcgatgg tgacgtgggc gtcatggcct cgcactatgc
     2761 gtccaagaca ccggcgccca actttcccgc cttgtcggat gccgacggcg cctgcacgcg
     2821 cgccaagttc aatgcgctgc tggcgtccag ctttggcaag gtggtgggct acaaggcggg
     2881 cctgaccaac ccggcggtgc aaaagcgttt caacaccgac aagccggtgt ggggcaagct
     2941 ctacgagggc atgatcgagc aaagcggcgc cagcgtgccg gccgcctttg gcgcgcgccc
     3001 tttgttcgag gccgacatgc tggtgcgcgt gaaggacgcg gccatcaacc aggccagcac
     3061 gccgtacgag gtgctgcagc atatcgacca ggtgatcccc ttcatcgagc tgcccgacct
     3121 ggtggtgcag gcgccggcgc agctcaacgg cccgggcgtg gccgccatca acgtgggcgc
     3181 gcgtctgggc gtggcgggcc agccgattgc cgtgcccgcg acgcgcggcg agcgctatgc
     3241 gctgctcgat gcgctggagc gcatgcaggt gcagctcacc aacaaccagg gccagctgct
     3301 ggccagcggc aagggcagcg acatcctggg ccagccgctc aatgccgtgg tctggatcgc
     3361 gcaggcgctg caaaaggacg gcatcacgct gcagccgggc gacctgatca gcctgggcag
     3421 cttctcgccg ctgctgccgc cacgcccggg cctgagcgtc acggccagct atgtcggcct
     3481 gcccggcgca cagcccgtgc aggtgcattt caagcaatga cgaacaccga cctcgccgcg
     3541 cagacccatg cgcgactgac gccgcagatg cgcagcgtgg tagaccgcct ggcacgcgcc
     3601 ggtcggcctg cactgcatac cttgagcccg gcccaggcgc gcatcgccta cgaggctggt
     3661 gccggcgtgc tggaggtgcc cagggccgag ctgccgcggg tcgaggatct gcatgtcccc
     3721 agccgtgatg gcgtgcagct gcccgcgcgc ctgtatgcgc ccggcaccgc ggccgcgctg
     3781 ccggcgctgc tgtacctgca tggtggcggc ttcaccattg gcagcgtggc cacgcatgat
     3841 gtgctgtgcc gcgagctggc gcgcctgggc cgctgcatgg tgctgtcgct cgattaccgc
     3901 ctggcgcccg agcacgcctt tcccaccgcc agcaacgacg cctgggacgc gctcgcctgg
     3961 ctggccgcca acgccgcccg gctgggtgca gaccccggcc gcctggccgt gggcggtgac
     4021 agcgcgggcg gcacgttggc ggccgtgaac gccatcctgg cgcgcgacgc gggcctgccg
     4081 ctggcgctgc agctgctcat ctacccaggc accacggcgc accaggacac gctgtcgcat
     4141 accgagttcg cgcacggccc gatactcgac cgcgccgcca taggctggtt cttcgaccag
     4201 tacattccca gccgcgccga gcgcgaggac tggcgctttg ccccgctgct ggcgcccgat
     4261 gtggacggcg tggcaccggc ctggataggc ctggccgaat gcgacccgct ggtggacgag
     4321 ggcgtcgaat atggcgacaa gctgcgcgcc gccggcgtgg ccgtggatct cgagatctac
     4381 cgcggcgtga cgcacgaatt catcaagatg ggccgcgcca ttgccgaggc acggcgcgcc
     4441 caccaggacg cggcgcgcgc actgcgcgcc gcctttggca tcgacggagg ctaagcccat
     4501 gcaacgccag gacttccgct gttttcaccg cctgcgcgtg cgctgggccg aggtggacat
     4561 ccagaaaatc gtcttcaacg cccattacct gacctatgcc gactgcgcca tgaccgacta
     4621 ctggcgcgcc ctggccctgc cttacgagag cggcatgcag gccctgggcg gcgaggtgta
     4681 tttgaagaag gccagcgtcg aataccacgc ctcggcgcgc ctggacgacc tgctggacgt
     4741 gggcatgcgc tgcgcgcgcg tgggccatac ctcgctgctg ttcgaatgcg ccatgttcgc
     4801 cggcgaccgc ctgctggcca cggtggagct ggtctacgtg tttgccgacc cggccacgca
     4861 gaccaagcgc cccgtaccgc ccgcgctgcg cgccatgatc gagcactatg aggccggtgg
     4921 cgagatggtg gaactgcgcg tgggcgactg ggccacgctg ggcgccgatg ccacggcact
     4981 gcgcatggcg gtgttcgtgc aggagcaggg catagacccc gccatagagc tggacgcgca
     5041 cgaccccacg gccgtgcacg ccgtggccta taaccgcctg gcccagcccg tggccaccgg
     5101 ccgcctgttg tacgaggcac cgggcgacgc gcgcataggc cgcatggccg tcgatcgcgt
     5161 ggtgcgcggc cagcgctggg gccgcgcggt gctcgatgct ctggtgcggg ccgcacaggc
     5221 gcgcggcgac gcccaggtga cgctgcacgc gcagtgcagc gccgaaggct tttaccgccg
     5281 cgccggcttt gtgcccgtgg gcgcggtgtt cgaggaggcg ggcatcgccc acatcaccat
     5341 gcggcgcacg cttgcttagc tattttattg atagctgttt gcgcttatct ggtaagcgcc
     5401 aaagcctttt ttggcttgaa acctgcaggg tggacgagtg gatttacttg agcagctcct
     5461 gcacctggtc gagcgccgcc ttggcgcatt gctcgtccag atggccgcca ggggcgccgc
     5521 cgatgcccac ggcgcctatc acctcattgc ccgccttgac cggcaggccg ccgcccagca
     5581 gcagatagcc tggaatctgg cccaggttgg ccgcgccagg gttcttttgc gaggtttcca
     5641 tgatggcgct ggtggcgttc ttggccgatg ctgaggtgta ggccttcagg cggctggcct
     5701 ccagggtgtg cgggccggcg ttgtcggcgc gcagctgcgc gcgcacctgg ccggcgcgat
     5761 cgaccacggt ggccgtcacc gcgtagccat tggcggcgca ggcggcaacg ctgtgcgtgg
     5821 cgatctggct ggcgaggtcc agcgacatgt ttttctcgct gcgcacggcc tgggcgttgg
     5881 cggcgccggc gatgacggcg gtggcgagca gggcgaaggt cttggcttgc atggcggggc
     5941 tttctcgggt gggttgaaca aggctgcagc ggcgctgcag gcatgggtgc aactgtagaa
     6001 aatcccccgc cggcgcgcca ttcgcacggc tacgctgggc actacgtagt tctacgcggc
     6061 gggcggcgcg tccatttccg ccaggctggc gtagcggcgc acgaggtgcg ccaggctggg
     6121 cacctgcagc ttggcgaaca ggttggcgcg gtgcacctcc acggtgcgtg gcgacagctc
     6181 cagcacccgc gccatgtcct tgttcgacag gccctgcacg atcagcgcca gcacctggcg
     6241 ctcgcgcggc gagagttcgg caaagcgctg ctgggcatcg cgatccgcct ggcgctgctg
     6301 gcgcgcgcgc acatgctggc gcacggcagc ctgcagggtc tccagcagca gttcgtcgtc
     6361 caccggcttt tccagaaact ccgccgcgcc ggccttgaac gcacgccggc acatgtccac
     6421 cgtgccgtgg ccagtcagca ggacgatggg gtgatccacc ccttgctcca ccagctgcgc
     6481 cagcagcgcc aggccaccga tgccgggcat gcgcacgtcg agcacgacgg cgcctatgcc
     6541 ctggcggtcg aactgcgtgc tgaaggccag ggggtcggcc caggtctgca cgcgcaggcc
     6601 tatggtggag atgagcaggg ccaggctgtt gcgcacggcg tcgtcatcgt ccaccaggtg
     6661 gatcagcggg gagaggtcgg cgttggcggc gatcatcttg tgttgtgtac cggcagacgc
     6721 agggcaaagc aggcgccggg gccggcatcg tgcagctgca ggctgccgcc catggcctcg
     6781 gccaggcttt gggaaagtgt caggcccagg cccaggccgc gatcgcggcc ggtggcaaag
     6841 ggggtgaaca ggcgtggccg catgtcgggg gcaatgccgg ggccgctgtc gcgcaccgtg
     6901 agcagcagct gtgcgccctg gtgcgtgagc gtgatccaca gctggcgttc gcccacgggc
     6961 gtggtgtcca tcgcctgcag cgcgttggtc agcaggttgt gcaggatttg ttgcaacgcc
     7021 accgcatcgg cacgggccgc aggcaggccg ggggcgaact gcgggtgcag atgtaccttg
     7081 tgggcggaca gctgcggttc gagcaggtgc agcgcatcga tgacggcctg gggcaggtcg
     7141 atggcgctgc tctgcatggc tgcgtcgggg cgctcgacca ggcggcgcag cctggtcacc
     7201 acatccgatg cgcgccgcgc ctgcgccacc acctgcgcca gggcctggcg cagcagcggc
     7261 atgtcgggcg cgtcctcggc cagcaggcgc tgcgcggcct ggctgctggc aagcaccgcc
     7321 gtgagtggct ggttgagctc gtgcgccatg ccggcggcaa gctcgcccag ggtgttcaag
     7381 cgcgccacct ggcccagctg cagcagctgc tcggcgcgcc ggcgcgccac gcgttggcgc
     7441 agcagctggc gcagcgccgt gagcagggcg gcgctgcccg ccaaccagcc cagcatgctg
     7501 ccccacggca gcatggccgc gcgcaccgcc tgctgcatgt gcagcacaag gggctggctt
     7561 gtggcggcca gcgtttttgc gctctgccac tgccagcgtg cagtgttttc cgcggcgccc
     7621 tggatggtgt agcgttgacc atcgagcgcg agccaggcgt gcaccgggct gccgggcgac
     7681 agcggccagt cgtcggccag ggccgtggtg cgcagatcca ggcgcagggc aaagctggct
     7741 ggtgttccgg cctgcaccag gtagagctgg ccaccggtca gattgctcgg agccagcacg
     7801 gcgtggccgc tggcgcggga ttgtgcgagt gccgcatcca tgcccggcgg ccagccagca
     7861 ggccaggcgg gcgcgccatg gtcatggcgc agcacttcgc gcacctgtgg gtaggggcgg
     7921 gtgagggcgc tccagggggc cggctcggtg gcgcggcttt caggttgcag caaaaccagc
     7981 gtggccagga tggcctcgtg ctgcaccatg cgctggctga gcacgcggtg ggcaatgcgc
     8041 gcgtcggtgt caaaactgtc atgcagttgc tgcaggcgct gttgcgccag ccagacgcag
     8101 ccggctgcgc ccagcaccag ccaggccagc caccagggcg ccatgcgtcg caatccgttc
     8161 atgggtaaat tctggcacgc ggcttcacgg caccggcggc tgcatcacgc gctcgtactg
     8221 cgtgggtgcc aggcgcgcca ccagcacggc caccagcagc gccatggcgc actgcagcca
     8281 ccagccaccg gagaacccgc cgccgccctg gtgcagccgc gccatcagcc agggcggcag
     8341 ggccgagagc agaaagccgc cgccctgcat gagcgcgttc aacgcgccgg cctgcagcgg
     8401 cgcgcgcaaa tggtccagtg ccaccagcat gaagaaggtg aagcagccgc ccaggcccac
     8461 gcccagcacg atggccgtgg ccagtggcgc ggcaagaggt gccatggcca gcagcgcaaa
     8521 gcccagcgcc tggcagccca gggtgaacca tagccagggc cggcggtctg gcccgccgcg
     8581 cgcggccagc accggcagca gcagggccgc tgccgcctga gcgatcgaca gcagggcgac
     8641 cagcgtgccg ctctgcgcgg cgctccagcc cagctcctgg tagtggggcg ccagccacgc
     8701 aatcatgctg gcgtagccgc cgttcatcag gccaaaggcc agcatcagat accaggtgcg
     8761 cgggcggcgc agcagccagg cgctggcgcc acgctgcgtg gcgccggccg cgggcgtcgc
     8821 catgcggccc agctgccacc aggccagcgc cagcgccgcc agcaccggca gcgtccacag
     8881 cgccagggcg ccgctccagc ccaggcccca ctgcagcgcc agtggtgaca gctgcgcgcc
     8941 taaagcgccg ccgcccatga gggaggccga gtacacgccc atcatcggtg ccacgtggcg
     9001 tggcgtgtgc agcttgatca ggcccggcag cgtgccctgt accagcgcca cgcccacgcc
     9061 gcacagcgcg gcggtggcca gcagccaggc gggcaccagg cgcagcgcgc aacccagggc
     9121 cagcagcgtg agcgccagcg ccagcgtggg tcgtgcgccc aggcggggca gtagcgcagg
     9181 cgccagccag gtgcccgcgc ccatcagggc catgggcagc agcgtgagcc aggccagcgc
     9241 ctgcagtccc agcccggtgt gcgcctgtat ggtcggcgtg agcggcccga tggcggtgag
     9301 aaagggccgc aggttcagcg tgaccagcac caccgtggcc agccaggcca gcgcgatgcg
     9361 agggcgctca gccacggcgg cgttgcagcc tgaacgcgct caaatgtctt ccagcagcgc
     9421 gtagggcagg ggcagcagcg tcagcggcac gccctgcgcg gcgcccacct gcagcgcccc
     9481 ggcagtggcg gcaatctgca gcgacaccag cgcatccacg ccgccaccgg gcgcagcggc
     9541 agcctgcacc accaggccca cgggctgctc ggcgtcatcc tgtgcaaaca cctcggcacc
     9601 gacggcgacc ggggccggcg catgggccag gtaggcgcgg cgcttgagcg tgccacggaa
     9661 ctggctgcgc gccaccacct cctggccggg gtagcagccc ttcttgaagt
//
LOCUS       DAOMTQ010000116         9678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1408_length_9678_cov_27.656032, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000116 DAOMTQ010000000
VERSION     DAOMTQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_9678_cov_27.656032"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PK518_11215"
     CDS             <1..522
                     /locus_tag="PK518_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRM49529.1"
                     /translation="QKRGVQMNMAYHGRQVMLTYEMPLGEIVLDFFDKLKSVSRGYAS
                     MDYEFKEYRASDVVKVDILLNGEKVDALSIIVHRTQAAYRGRAVAAKMREIISRQMFD
                     VAIQAAIGANIIARETIKALRKNVLAKCYGGDITRKRKLLEKQKAGKKRMKQIGSVEV
                     PQEAFLAILQVEE"
     gene            527..1501
                     /gene="lepB"
                     /locus_tag="PK518_11220"
     CDS             527..1501
                     /gene="lepB"
                     /locus_tag="PK518_11220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM49530.1"
                     /translation="MQAMQYITAAVLAAFVSYIGAWYMGAIEGNFALLLLLATVVTGA
                     YWLAERIVFLPQRRQAARAIEDAAAQRRAELDRMGIKKVDGDVAEAKGRILMQPWWLD
                     WTAGLFPVIAVVFLLRSFLFEPFKIPSGSMIPTLLVGDLILVNKFTYGLRLPVINTKI
                     TQGNPVQRGDVMVFRYPPQPSMDYIKRVVGLPGDEVAYLDKRLTVNGQPVPTTALPDF
                     FEQDSMRYFRQFEEQLGTHKHRLLNSPEAPAFVQGASNFKFRDQCRYSVEGVTCKVPE
                     GHYFLMGDNRDNSLDSRYWGFVPDENIVGKAFFVWMNFGNLKRIGSFN"
     gene            1529..1894
                     /locus_tag="PK518_11225"
     CDS             1529..1894
                     /locus_tag="PK518_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HRM49531.1"
                     /translation="MGLHRSATRARQRGLSFIGVIFVGLIAVAAFAIGGQSVPVFLEY
                     ANIKKAAAKAARDGNTVPEIRAAFDRAAQIDDIHSISGKDLEITKRNDKVVVSFNYSR
                     EIPLAGPAYLVYRFQESTN"
     gene            1894..2580
                     /gene="rnc"
                     /locus_tag="PK518_11230"
     CDS             1894..2580
                     /gene="rnc"
                     /locus_tag="PK518_11230"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRM49532.1"
                     /translation="MPADSLSALQHRLQHRFSDPSLLQRAVTHRSYSSEHNERLEFLG
                     DSVLNLAVSSLLFRRMQGLPEGELSRVRALLVKQDSLHRIAMRLQLPQVLRLGEGESK
                     SGGKLRPSILADALEAIIGAVYLDAGYDQAEALVHRLLEGVELSPRLQEAAKDAKTAL
                     QEWLQGRKMKLPQYQVIDTTGAAHNQVFHVECLVAELQLTARGSGASRRAAEQAAASA
                     MLAQLKVKQA"
     gene            2577..3608
                     /gene="era"
                     /locus_tag="PK518_11235"
     CDS             2577..3608
                     /gene="era"
                     /locus_tag="PK518_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRM49533.1"
                     /translation="MNAMNTEAGNAHESGASGQIDQNQQNDLEAMLAAARPGTGAPAD
                     AAGQRCGLIAIVGKPNVGKSTLMNALVGQKISITSRKAQTTRHRITGIRTRGATQFVF
                     VDTPGFQTRHSTALNKSLNKTVMGAIGDVDLILFVVEAGSFTLADAKVLSLFKPGIPT
                     LLIANKLDTVHRRADIAPWLKSMQERHPFAEFVPMSAKKRDDIERLYGICEKYLPEQA
                     WWYAEDELTDRSEKFLASETVREKLFRFTGDELPYTSTVVIDKFDEEASKQHKRFLRI
                     AATIVVERDSHKAMIIGEKGERLKRISTEARQELEKLMDAKVFLEVWVKVRSGWADDE
                     ARVRSFGYE"
     gene            3712..4497
                     /gene="recO"
                     /locus_tag="PK518_11240"
     CDS             3712..4497
                     /gene="recO"
                     /locus_tag="PK518_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRM49534.1"
                     /translation="MATRRIADEPAFILHSYDWSESSLILECFTRHHGRVALVAKGAK
                     KPTSNFRPVLLPLQPLRLGWTWGGEVGQGEIHALKGAEWVGGHVMPVGDALLSGLYLN
                     ELLMRLLAREDTHAPLFDLYAGVVRVLGSGEHGEALEPVLRAFELLLLREIGLLPALD
                     TETATLAPLADASRYTLLAEGGLRAATPDDRAALSGAQWQALQRSLDEDGAASYGAVL
                     RVVAGRGFAAAAELKAQLRALLQYHCGSPLLRTRQLMMDLQSL"
     gene            4494..5273
                     /locus_tag="PK518_11245"
     CDS             4494..5273
                     /locus_tag="PK518_11245"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRM49535.1"
                     /translation="MTTHPRTALSVNVNKVALVRNTRHLGIPSVLRAAQLCLEAGADG
                     ITVHPRPDERHIRAQDVYELAELLGQWPEAEFNIEGNPTHNLMEFIRQVRPHQATFVP
                     DSVGQFTSDHGWNFPQDAERLEPLIQECRELGVRVSLFMDPIPEQMAAARAVGADRVE
                     LYTEPYAAAWATPQRSDQLARYRLAAQAAITAGLGVNAGHDLNRDNLADFLAAVPGVL
                     EVSIGHALIADALELGYADTVTAYQRCIDQGMAERALSERR"
     gene            5308..5700
                     /gene="acpS"
                     /locus_tag="PK518_11250"
     CDS             5308..5700
                     /gene="acpS"
                     /locus_tag="PK518_11250"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRM49536.1"
                     /translation="MIYGIGTDICDVRRIRASVERHGERFAAKVLADAELAAWRQRSS
                     RWPERGLRYLATRFSAKEAFSKAIGLGMRMPMTWRHCEVANLPTGQPVIVLHGALKQW
                     FEARGLRAHLSVTDESDYAASFCVVEKI"
     gene            5767..6849
                     /gene="nagZ"
                     /locus_tag="PK518_11255"
     CDS             5767..6849
                     /gene="nagZ"
                     /locus_tag="PK518_11255"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRM49537.1"
                     /translation="MTEHAPLIIDVAGTGLSDTDRRRLAHPLVGGIILFARNWQDRAQ
                     LLQLTAAIKAVRHDLLICVDHEGGRVQRFRTDGFTHVPPMRALGELWMNDGKAGQGSG
                     ALRAMDAATAAGYVLGAELRACGVDFSFTPVLDLDWGESGVIGDRAFARDPRVVAMLA
                     RALMQGLLQAGMANCGKHFPGHGFVKADSHTEIPIDRRSLKAILAEDAAPYPWLSTVL
                     TSVMPAHVIYPKIDARPAGFSAKWLQDILRGRLRFDGAIFSDDLSMEGARRLDGQVVS
                     YTDAGVAALNAGCDLVLLCNQSLEGGAGVDELIAGLATAQAQGRWQASAASEARRLAL
                     LPETLPQPWDDLMVQPAYMHALDLLP"
     gene            6852..7904
                     /locus_tag="PK518_11260"
     CDS             6852..7904
                     /locus_tag="PK518_11260"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRM49538.1"
                     /translation="MRVGVLTGGGDCPGLNAVIRAVTKSLIHHGHCEVLGIADGFEGL
                     MGQTPRTLPLAWTQVSGILHMGGTILGTSNSANPLKDEATLAQVGRNVRALGLDVVVA
                     IGGDGTMSLAHGLRQVGLACVGVPKTIDNDIALCERSFGFDTAVATATEALHRVESTA
                     NSHHRVMIVETMGRHAGWLALEAGIAGAADIILLPEIDYDLQAIVQRCREREQRQRYT
                     IICIGEGAKPRGGALTVREHVAGSPDPVRLGGVGHVLRQQLQPHLKSEVRATVLGYVQ
                     RGGDPTPFDRVLATRFGHHAAQLVIDGQLGRMVTLQNGGIGSVEIAQVANTQRTVPPG
                     HELITVARDIGVCFGD"
     gene            8034..9230
                     /locus_tag="PK518_11265"
     CDS             8034..9230
                     /locus_tag="PK518_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRM49539.1"
                     /translation="MQLHQISDKPQPVAVLPGWPVLRLGFRPFYLTSALLACLSVPVW
                     AAVFLGYFDMKLELSPLLWHGHEMLFGFAAGVIAGFLLTAVKAWTGLETARGPLLGTL
                     ALVWVAARIAALVAPYPVFMGLDMALLPAVALVLLRVLIKSGNKRNIPLVSLLVLMSI
                     ANLVFHLSVLGAIAVPAVAPLYAQLALVIMVICVMTGRVVPMFTKNVTPGLVINVSRR
                     FELTLLTVTAATLALWVLGAPAPLVGVLSAVAAVLHAARLWQWHPRVTLKRPILWILH
                     ASYAWMPMGFALLALAQWGLVGVSLAVHAFAIGVIGGLIIGMITRTARGHTGRPLLAS
                     RGEVVAYALVLLAAVARVLVPALQPAWYAYAVEAAACLWAVAFAIYLVIYTPWLTQTR
                     LDGKDG"
     gene            9279..9566
                     /locus_tag="PK518_11270"
     CDS             9279..9566
                     /locus_tag="PK518_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRM49540.1"
                     /translation="MSSTVAEIDVRTIAPYERHAQLFARFDALQTGEAFVIVNDHNPV
                     PLHMQLEGRAPGQVAWTYLQQGPDLWRVQIGKQAASKHGVEGSCCSGGACG"
BASE COUNT         1619 a         3207 c         3219 g         1633 t
ORIGIN      
        1 cagaagcgcg gcgtgcagat gaacatggcc taccacggcc gccaggtcat gctgacctat
       61 gagatgccac tgggcgagat cgtgctggac ttcttcgaca agctcaagtc ggtgtcgcgt
      121 ggctatgcgt cgatggacta cgagttcaag gagtaccgcg cctcggacgt ggtcaaggtg
      181 gacatcctgc tcaatggcga gaaggtcgat gccctgtcca tcatcgtgca ccgcacgcag
      241 gcggcctacc gcggccgtgc cgtggcggcg aagatgcgcg agatcatcag ccgccagatg
      301 ttcgatgtgg ccatacaggc ggcgatcggc gccaacatca tcgcgcgcga gaccatcaag
      361 gcgctgcgca agaacgtgct cgccaagtgc tatggcggcg acatcacgcg caagaggaag
      421 ctgctggaaa agcaaaaggc gggcaagaag cgcatgaagc agatcggctc ggtggaagtg
      481 ccccaggagg ccttcctggc catcctgcaa gtggaggaat gacagcgtgc aagccatgca
      541 atacatcacg gccgcggtgc tcgcggcgtt tgtgagctat atcggcgcct ggtatatggg
      601 cgccatcgag ggcaatttcg cgctgctgct gctgctggcc accgtggtca cgggcgccta
      661 ctggctggcc gaacgcattg tgtttctgcc gcagcggcgc caggcggcgc gggcgatcga
      721 ggatgccgcg gcgcagcgcc gtgccgagct tgaccgcatg ggcatcaaga aggtcgatgg
      781 cgacgtggcc gaggccaagg gccgcatcct catgcagccc tggtggctgg attggacggc
      841 aggcctgttc ccggtgattg ccgtggtgtt cctgctgcgc tccttcctgt tcgagccctt
      901 caagatcccc tcgggctcga tgattcccac gctgctggtg ggcgacctga ttttggtgaa
      961 caagttcacc tacggcctgc gcctgccggt gatcaacacc aagatcacgc agggcaatcc
     1021 ggtgcagcgc ggcgatgtga tggtgttccg ctacccgccc cagccgagca tggactacat
     1081 caagcgcgtc gtcggcctgc ccggcgacga ggtggcgtat ctggacaagc gcctcacggt
     1141 caacggccaa cccgttccga ccacggcgct gccggacttt ttcgagcagg acagcatgcg
     1201 ctacttccgc cagttcgagg agcaactggg cacgcacaag caccgtttgt tgaacagccc
     1261 cgaggccccg gccttcgtgc agggggcgag caatttcaag ttccgcgatc agtgccgata
     1321 tagcgtggag ggcgtcacct gcaaggtgcc cgagggccac tatttcctga tgggtgacaa
     1381 ccgtgataat tcgctcgatt cgcgctactg gggcttcgtg cccgacgaga acatcgttgg
     1441 caaggccttc ttcgtgtgga tgaattttgg caacctcaag cgcatcgggt cgttcaacta
     1501 gtccagctac acagagggag attcaggtat gggtttgcac cgcagcgcaa cgcgtgcgcg
     1561 ccaacggggg ctgtcgttca ttggcgtgat ctttgtcggc ctgatcgccg tggcggcatt
     1621 tgccattggc gggcagtcgg tgccggtgtt cctcgaatac gccaacatca agaaggcggc
     1681 cgccaaggcc gcgcgcgacg gcaacacggt gcccgagatc cgcgccgcat tcgaccgcgc
     1741 ggcgcagatt gacgacatcc attccatttc cggcaaggac ctggagatca ccaagcgcaa
     1801 cgacaaggtg gtggtgtcgt tcaactactc gcgcgagatc ccgctggcgg ggccggccta
     1861 cctggtctac cgcttccaag aatcgacgaa ttagtgccag ccgacagtct ctctgcgctg
     1921 cagcatcggt tgcagcaccg gttctcagac ccctcgctgc tgcagcgtgc cgtcacgcat
     1981 cgcagctact cctcagagca caacgaacgc ctggaattcc ttggcgactc ggtgctgaac
     2041 ctggccgtct ccagcctgct gttcaggcgc atgcagggcc tgcccgaggg tgagctctcg
     2101 cgcgtgcgtg ccctgctggt caagcaggac tcgctgcacc gcattgccat gcgcctgcag
     2161 ctgccccagg tgctgcgcct gggcgagggc gagtccaagt ccggcggcaa gctgcgtccg
     2221 tccatcctgg ccgacgcgct ggaggccatc atcggcgctg tctatctgga cgcgggctac
     2281 gaccaggccg aggccctggt gcaccgcctg ttagagggcg tggagctgag cccgcgcctg
     2341 caggaggccg ccaaggacgc caagaccgca ttgcaggagt ggctgcaggg tcgcaaaatg
     2401 aagctgccgc agtaccaggt gatagacacc acgggcgcgg cgcacaacca ggtgttccat
     2461 gtcgagtgcc tggtggccga gctgcaactg acagcgcgcg gcagcggcgc atcgcgccgc
     2521 gcggcagagc aggccgccgc cagcgccatg ctggcccaac tgaaagtgaa acaggcatga
     2581 atgctatgaa tactgaagct ggtaacgccc atgagtcggg cgccagcggc caaatagacc
     2641 aaaaccaaca gaacgacctg gaggccatgc tggccgccgc gcgccccggc accggcgcgc
     2701 cagccgacgc ggccggtcag cgctgcggcc tgatcgccat cgtcggcaag cccaacgtgg
     2761 gcaagtcgac gctgatgaac gccctggtcg gccagaagat cagcatcacc agccgcaagg
     2821 cgcagaccac gcgccaccgc atcaccggca tacgcacgcg cggtgccacg cagttcgtgt
     2881 ttgtcgatac ccccggcttt cagacccggc acagcacggc gctgaacaaa tcgctgaaca
     2941 agaccgtgat gggcgcgatc ggcgacgtgg atctgatcct gttcgtcgtc gaggccggca
     3001 gcttcaccct ggccgacgcc aaggtcttga gcctgttcaa gcccggcatc cccacgctgc
     3061 tcattgccaa caagctcgac accgtgcacc gccgcgccga catcgcgccc tggctcaaga
     3121 gcatgcagga gcgccacccg ttcgccgagt tcgtgcccat gtcggccaag aagcgtgacg
     3181 acatcgagcg cctctacggt atctgcgaaa aatacctgcc cgagcaggcc tggtggtacg
     3241 ccgaggacga gttgaccgac cgcagcgaga agtttctggc cagcgagacg gtgcgcgaaa
     3301 agctgtttcg cttcaccggc gacgagctgc cctacacctc caccgtggtc atcgacaagt
     3361 tcgacgaaga ggcgagcaag cagcacaaac gctttctgcg catcgcggcc accatcgtgg
     3421 tcgagcgcga cagccacaag gccatgatca tcggtgagaa gggcgagcgc ctgaagcgca
     3481 tcagcaccga ggcgcgccag gagctggaaa agctcatgga cgccaaggtg ttcctggagg
     3541 tctgggtcaa ggtgcgctcg ggctgggccg acgacgaggc ccgcgtgcgc tcgtttggct
     3601 atgagtgatt ggcgagaagt aagtggtgaa tggtgagtgg tgagtggcag attcgcctcg
     3661 cgcccattca ccactcacga cttaccactc accacttacc cccccctaaa aatggccacg
     3721 cgccgcattg ccgacgagcc cgccttcatt ctgcacagct atgactggag cgagtccagc
     3781 ctgatcctgg aatgcttcac gcgccaccat gggcgcgtgg cgctggtcgc caagggggcg
     3841 aagaagccga cctccaactt ccgcccggtg ctgctgccgc tgcagccgct gcgcctgggc
     3901 tggacctggg gcggcgaggt agggcagggc gagatccatg cgctcaaggg cgcggaatgg
     3961 gtgggtggcc atgtcatgcc ggtgggcgat gcgctgctgt cgggcctgta cctgaacgag
     4021 ctgctgatgc gcctgctggc gcgcgaggac acccatgccc cgctgttcga tctgtacgcc
     4081 ggcgtggtgc gcgtgctggg cagtggcgag catggcgagg cgctggagcc ggtgctgcgc
     4141 gccttcgagc tgttgctgct gcgcgaaatc ggcttgctgc ccgcgctcga caccgagacc
     4201 gccacgctgg cgccgctggc cgacgcatcg cgctacaccc tgctggccga gggtggcctg
     4261 cgcgccgcca cgccggacga ccgcgcggcg ctctccggcg cgcagtggca ggcgctgcag
     4321 cggtcgctgg acgaggatgg ggcggccagc tatggcgccg tgctgcgcgt tgttgccggc
     4381 cgtggatttg ccgctgcggc cgagctcaag gcccaattgc gggcgctgct gcaataccat
     4441 tgcggcagcc ctttgctgcg cacgcgccag ctcatgatgg acttgcaatc gctatgacca
     4501 cacaccctcg caccgccctc tcggtcaatg tcaacaaggt cgccctggtg cgcaacacgc
     4561 gccacctggg catacccagc gtcctgcgcg ccgcccagct gtgcctggag gcgggggccg
     4621 acggcatcac cgtgcacccc cggcccgatg agcgtcatat ccgcgcgcaa gacgtgtacg
     4681 agctggccga gctgctcggc cagtggcccg aggcggagtt caacatcgag ggcaacccca
     4741 cgcacaacct gatggaattc atccggcagg tgcgcccgca tcaggccacc ttcgtgcccg
     4801 acagcgtggg ccagttcacc agtgaccatg gctggaactt tccccaggac gccgagcgcc
     4861 tggagccgct gatccaggag tgccgcgagc tgggcgtgcg cgtgagcctg ttcatggacc
     4921 cgatccctga gcagatggcc gccgcccgcg ccgtcggcgc cgaccgcgtg gagctgtaca
     4981 ccgagcccta cgccgccgcc tgggccacgc cccagcgctc ggatcagctg gcgcgctacc
     5041 gcctggcggc gcaggctgcc atcaccgccg gcctgggcgt gaacgccggc catgacctga
     5101 accgggacaa cctggcggac ttcctggccg ccgtgccagg cgtgctggag gtgtcgatag
     5161 gccacgcgct gatcgccgac gcgctggagc tgggctacgc cgacaccgtc accgcctatc
     5221 agcgctgcat agaccagggc atggcggaac gcgccctgag cgagcggcga taagccttcg
     5281 cccaacgccc aacgcccaac gcccaacatg atctacggca taggcaccga catctgcgac
     5341 gtgcgccgca tacgcgccag cgtggagcgc catggcgagc gctttgccgc caaggtgctg
     5401 gccgacgccg agctggccgc ctggcgccag cgcagcagcc gctggcccga gcgcggcctg
     5461 cgttacctgg ccacgcgctt ttccgccaag gaggccttca gcaaggccat aggcctcggc
     5521 atgcgcatgc ccatgacctg gcgccattgc gaggtggcca acctgcccac cggccagccg
     5581 gtgatcgtgc tgcatggcgc gctcaagcaa tggtttgagg cgcgcggtct gcgcgcccac
     5641 ctgagcgtga ccgacgagag cgattacgcg gcgagcttct gcgtggttga gaagatttaa
     5701 gcaaaaaacg gctctagcgc ctatccataa agcgcaaaca gctatcaaaa cagaagtaat
     5761 ttgagaatga ctgaacacgc ccccctgatc atcgatgtgg ccggcaccgg cctgagcgac
     5821 accgaccgcc gccgcctggc ccacccgctc gtcggcggca tcatcctgtt tgcgcgcaac
     5881 tggcaggaca gggcccagct gctgcaactc accgccgcca tcaaggccgt gcgccatgac
     5941 ctgttgatct gtgtcgatca cgagggcggg cgcgtgcagc gttttcgcac cgatggcttc
     6001 acgcatgtcc cgcccatgcg cgccctgggc gagctgtgga tgaatgacgg caaagcaggg
     6061 cagggcagcg gcgccctgcg cgccatggat gcggccacgg ccgccggcta tgtgctgggc
     6121 gccgagctgc gcgcctgcgg cgtcgatttc agcttcaccc cggtgctgga cttggactgg
     6181 ggcgagagcg gcgtgattgg cgaccgcgcc ttcgcccgcg acccgcgcgt cgtcgccatg
     6241 ctggcgcgcg ccctgatgca gggcctgctg caggcgggca tggccaactg cggcaagcat
     6301 ttccccggcc acggcttcgt caaggcggat tcgcacaccg agattccgat cgacaggcgc
     6361 agcctcaagg ccatcctggc cgaagatgcc gcgccctatc cgtggctgtc cacggtgctc
     6421 accagcgtca tgccggcaca tgtcatctac cccaagatcg atgctcgccc ggcgggcttc
     6481 tcggccaagt ggctgcagga catactgcgc ggccggttgc gctttgacgg cgccatcttc
     6541 agcgacgacc tgagcatgga aggcgcgcgc cgcctggacg gacaggtcgt gagctacacc
     6601 gacgccggcg tggccgcgct gaacgccggc tgcgacctgg tgctgctgtg caaccagagc
     6661 ctggagggcg gcgccggggt ggacgagctg atagccggcc tggccaccgc ccaggcgcag
     6721 ggccgctggc aggccagcgc cgccagcgag gcgcgccgcc tggccctgct gcccgagacc
     6781 ctgccccagc cctgggacga cctcatggtg cagccagcct acatgcacgc gctcgatctg
     6841 ctgccatgaa catgcgtgtc ggtgtgctga ctggcggggg cgactgcccc ggcctgaacg
     6901 cggtgatccg cgccgtcacc aagtcgctga tccaccatgg ccactgcgag gtgctgggca
     6961 ttgccgacgg cttcgagggc ctgatgggcc agaccccgcg cacgctgccg ctggcgtgga
     7021 cgcaggtctc gggcattttg cacatgggcg gcaccatcct gggcaccagc aacagcgcca
     7081 acccgctcaa ggacgaggcc acgctcgccc aggtcgggcg caatgtgcgt gccctgggcc
     7141 tggatgtggt cgtggccata ggcggtgacg gcaccatgag cctggcccac ggcctgcgcc
     7201 aggtgggcct ggcctgcgtg ggcgtgccca agaccataga caacgacatc gcgctgtgcg
     7261 agcgcagctt tggcttcgac accgcggtgg ccacggccac cgaggcattg caccgcgtgg
     7321 agagcacggc caacagccac caccgcgtga tgatcgtcga gaccatgggc cgccacgccg
     7381 gctggctggc gctggaggcg ggcattgccg gcgcggccga catcatcctg ctgcccgaga
     7441 tcgactacga cctgcaggcc atagtccagc gctgccgcga gcgcgagcag cgccagcgct
     7501 acaccatcat ctgcataggc gagggcgcca agccgcgcgg tggcgccctg acggtgcgcg
     7561 agcatgtggc cggcagcccc gatccggtgc gcctgggcgg cgtgggccat gtactgcgcc
     7621 agcaactgca gccgcacctg aagagcgagg tgcgcgccac ggtgctgggc tatgtgcagc
     7681 gcggtggcga ccccacgccg tttgaccgcg tgctggccac gcgctttggc caccacgcgg
     7741 cgcagctggt catcgacggg cagctgggcc gcatggtcac gctgcaaaac ggtggcatag
     7801 gcagcgtgga gatcgcccag gtggccaaca cccagcgcac cgtgcccccc gggcacgagc
     7861 tgatcaccgt ggcgcgcgat attggcgtgt gttttggcga ttgaggccag gccgtgaggc
     7921 ctgccaggca gcacggtagg gcgtgaaaaa ttagggccgc gctaaagatt catgtaaaat
     7981 gaaacttata agtggttgcc gttccgggcg ttttccacgt ttcaagtttc gtgatgcaac
     8041 tccaccaaat ttccgataaa ccccagcccg tggccgtcct gccgggctgg cccgtgctgc
     8101 gcctggggtt caggcccttc tatctgacct cggccctgct ggcctgcctg tcggtaccgg
     8161 tatgggcggc ggtgttcctg ggctacttcg acatgaagct ggagctctcg cccctgctgt
     8221 ggcatgggca cgagatgctg ttcggctttg ccgccggggt gatcgccggc ttcctgctca
     8281 cggccgtcaa ggcctggacg gggctggaga cggcacgcgg gccgctgctg ggcaccctgg
     8341 ccctggtgtg ggtggcggcg cgcatcgcgg cgctggtggc gccgtatccg gtgttcatgg
     8401 ggctggacat ggccttgctg ccggccgtgg ccctggtgct gctgcgcgtg ctcatcaagt
     8461 ccggcaacaa gcgcaacatt ccgctggtgt cgctgctggt gctgatgtcc atcgccaatc
     8521 tggtgttcca tctgtcggtg ctgggcgcca ttgccgtgcc tgccgtggcg ccgctgtatg
     8581 cgcagctggc gctcgtcatc atggtgatat gcgtgatgac cgggcgcgtg gtgcccatgt
     8641 tcaccaagaa tgtcacgcca ggcctggtca tcaacgtgtc gcgcaggttc gaactcacgc
     8701 tgctcaccgt gacggccgcc accctggcgc tgtgggtgct gggcgcgccc gcgccgctgg
     8761 tcggcgtgct gagtgccgtg gccgcagtgc tgcatgccgc acggctgtgg cagtggcacc
     8821 cgcgggtgac gctcaagcgt cccatcctgt ggattctgca tgcctcttac gcctggatgc
     8881 ccatgggctt tgcgctgctg gcgctggcgc aatggggcct ggtgggggtg tcgctggccg
     8941 tgcatgcatt tgccatcggc gtgattggcg ggctcatcat cggcatgatc acccgcaccg
     9001 cgcgtggcca taccgggcgg cctttgctgg cctcgcgcgg cgaggtggtg gcctatgcac
     9061 tggtgctgct ggccgccgtg gcgcgcgtgc tggtgcctgc cctgcagccg gcctggtatg
     9121 cctacgccgt ggaggccgca gcctgcctgt gggccgtggc atttgcgatc tatctggtga
     9181 tctacacccc ctggctcacg caaacgcgcc tggatggcaa ggatggctga gccgtttttc
     9241 tttttttctc tccgactctt tacctgaaag gttgaaccat gagctccacc gttgccgaaa
     9301 tcgacgtgcg caccatcgcc ccctacgagc gccatgcgca gctgtttgcc cgctttgacg
     9361 ccctgcaaac cggcgaggcc ttcgtgatcg tgaacgacca caaccccgtg cccctgcaca
     9421 tgcagcttga aggccgcgca cctggccagg tggcctggac ttatctgcaa cagggcccgg
     9481 atctgtggcg cgtgcagatc ggcaagcagg cggcgtccaa gcatggcgtc gaaggctcct
     9541 gctgctcggg cggtgcctgc ggctgattcc gcttgtcaat caaatgagca cggggcaagc
     9601 atcacggctg tagttggcgt gcaagtccgc gtgcgcaccg tggcggccgt ccttggcacc
     9661 gctgcggtgc tcggccag
//
LOCUS       DAOMTQ010000117         9538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1444_length_9538_cov_26.242328, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000117 DAOMTQ010000000
VERSION     DAOMTQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_9538_cov_26.242328"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..177)
                     /locus_tag="PK518_11275"
     tRNA            complement(102..177)
                     /locus_tag="PK518_11275"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Glu,seq:ttc)
     gene            complement(195..270)
                     /locus_tag="PK518_11280"
     tRNA            complement(195..270)
                     /locus_tag="PK518_11280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Ala,seq:ggc)
     gene            complement(332..1753)
                     /gene="gltX"
                     /locus_tag="PK518_11285"
     CDS             complement(332..1753)
                     /gene="gltX"
                     /locus_tag="PK518_11285"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRM49541.1"
                     /translation="MTQAKVRTRFAPSPTGFIHLGNIRSALYPWAFARAMGGDFILRI
                     EDTDVERSTQASVDVILEGMAWLRLDHDEGPYYQMQRMARYREVLVQLQASGHVYPCY
                     MSMAELDALRERQTAAKEKPRYDGTWRPEPGKQLPPVPEGVQPVLRFKTPKDGVVAWD
                     DKCKGRIEFQNSELDDLVIARPAQNGEAVGTPTYNFCVCVDDIDMRITHVIRGDDHVN
                     NTPRQIHIFEALGAQVPVFAHLPTVLNEQGEKMSKRNGAKAVTQYRDEGYLPDAMVNY
                     LARLGWSHGDDEIFSRAQFLEWFNLDHLGRSAGQFDEAKLRWVNAQHMKALDDFELAR
                     LVAPFVENEGVAPAALDERLPRICALFKDRCETLVDLARWVRVFYLDRVERNAEDYAR
                     HVTPEAGPLLDAFATAMATVEWNKEAIAAAIKQLLKEQGAKMPQLAMPVRVLTVGQAH
                     TPSVDAVLELLGREKILTRLKSR"
     gene            complement(1750..2964)
                     /locus_tag="PK518_11290"
     CDS             complement(1750..2964)
                     /locus_tag="PK518_11290"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HRM49542.1"
                     /translation="MTEKSLPPGLHPDTLAVREAVERSQWGEHSEALYLSSSFVQSDC
                     ASSARRFANEEAGYTYSRTANPTVTSFERRLAAMEGTECAVATSTGMSAILLVALTVL
                     KAGDHVVCSQSMFGSTIKLLGTEMARFGVETSFVSQTDVAAWQAAIRPNTRLLFAETP
                     TNPLTDLCDIAALAEIAHAHGALLAVDNSFASPVLQQPARFGADIVVHSGTKLLDGQG
                     RVMAGAVCGTVALVDKVMGTFLRSGGLNLAPFNAWVVLKGLETLALRVKAESAAALEL
                     AAWLEQQPKVARVYYPGLKSHPQHELAMRQQNGMGGTVLAFDVAGQGAEQLRANAFHV
                     IDSTRVCSITANLGDVKTTITHPASTSHGRLTEEQRQAAGIGQGLVRISVGLEHLDDL
                     KADLLRGLDTLA"
     gene            complement(3093..4598)
                     /gene="purF"
                     /locus_tag="PK518_11295"
     CDS             complement(3093..4598)
                     /gene="purF"
                     /locus_tag="PK518_11295"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRM49543.1"
                     /translation="MCGIVGVVSTAPVNQLIYDALLLLQHRGQDAAGIVTQEGRKFFM
                     HKAKGMVRDVFRTRNMRALPGSIGLGQVRYPTAGSAASEEEAQPFYVNAPFGIVMVHN
                     GNLTNAKQLRRELAETDHRHTNTESDSEVLLNVLAHELARASSGASLTTDDVFAAVRA
                     VHQRIKGSYAVVALIAGYGLLAFRDPFGIRPLCMGRGADGTVMLASESVALEGTLHRL
                     ERDIAPGEAVFVQLDGTVQTRQCAQQPRLYPCVFEYVYLARPDSIMDGISVYQARLNM
                     GETLAKRVVSTVPPSQIDAIIPIPESSRPSAMQLAQLLGLPYREGFVKNRYVGRTFIM
                     PGQGTRKKSVRQKLNAIASEFKGKRVLLVDDSIVRGTTSKEIVQMARDAGAVKVYLAS
                     AAPPVRHPNVYGIDMPTRAELVAHDRTEEEIRQVIGADALIYQDVAAMKQAVGKINPG
                     VQGFEASCFDGLYITGDISDEEVTALNEGRQRGGEDDEEDTSRLSLPNAQE"
     gene            complement(4675..5163)
                     /locus_tag="PK518_11300"
     CDS             complement(4675..5163)
                     /locus_tag="PK518_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRM49544.1"
                     /translation="MAALDWVFLTVLAASLLIGAWRGLVFELLSLAGWVVAFFAAQWF
                     ADAAGAWLPMGEADAAWRHVAGFVVVFVAAVFACGLLAWLLKKLVDAVGLRPADRALG
                     ALFGVLRGLLLLLVVALVAGWLQVQDAPWWRQSQLAPWLQVALKGMRPVLPEELGRHL
                     PL"
     gene            complement(5191..6018)
                     /locus_tag="PK518_11305"
     CDS             complement(5191..6018)
                     /locus_tag="PK518_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRM49545.1"
                     /translation="MAFFKFRWPGGAEPQADSNKGARRSRAAAQAESIDDMRRRARHR
                     LIGAAVLVLAGVVGFPLVFDTQPRPVAVDVAIDIPDRNKVAPLAVPGKGASQDEPRAK
                     PEPAAAGLDAGEEVIGTSSRAEPAAQVEAKPEARTEPKPAPRPEPKAEPKPEPKVEHK
                     PEPKPDAKPARPDDAARARALLEGRGQQPAQAASAAAADQGRFIVQVGAFADADKVRE
                     VREKLERSGLKTYTQVVDTKDGKRTRVRLGPFDSRAEADKAVGRVKSAGLSASVLAL"
     gene            complement(6047..7372)
                     /gene="folC"
                     /locus_tag="PK518_11310"
     CDS             complement(6047..7372)
                     /gene="folC"
                     /locus_tag="PK518_11310"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HRM49546.1"
                     /translation="MQTYPQTLQGWLEHCERLHAKNIDMGLARVGEVARRLALRFDCP
                     VITVAGTNGKGSTCALLEAVAMEAGYRTGVYSSPHLVHFEERCRVRGDSVAAAALLPH
                     FEAVERARTEAGTEVSLTYFEFTTLAILRFMSQAGLDVAILEVGLGGRLDATNIIDAD
                     CAVITSVDIDHVEFLGSDRESIGREKAGIMRAGRPVVVSDPLPPQSVIDQAQQIGADL
                     WRFGQDFNYSGDKQQWSWAGRGRRYAGLAYPALRGANQLINASGALAALEALRQRLPV
                     TAQAVRLGLARVELPGRFQVVPGQPALVLDVAHNPHSVAALTANLDAMGYFPTTHAVF
                     GAMADKDLAPMLEKIAPLIDRWYFTDLPTPRAESAAGLQQKWSTLPAASATGRKAGTS
                     LHASPEEALRAAVEAADPTDRIVVFGSFYTVGGVLKNGVPRLHAKHLDA"
     gene            7421..7810
                     /locus_tag="PK518_11315"
     CDS             7421..7810
                     /locus_tag="PK518_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HRM49547.1"
                     /translation="MSTPTTIILYGIPNCDTVKKARAWLAGQSLAYQFHDFKKQGVPA
                     ERLSAWMAAVGWDRLLNRQGTSWRRLDAAVQSSVQDAASAAALMREHASVIKRPVVEW
                     NGTATGRVTVGFKPETWNELLAAGSKT"
     gene            7917..8573
                     /locus_tag="PK518_11320"
     CDS             7917..8573
                     /locus_tag="PK518_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRM49548.1"
                     /translation="MNKIIVLATLTMAAMASQAQDLGRVLSATPITQQVAVPQQVCGN
                     ETIYNGARPTTGGGAVLGAIAGGAAGNAIGSGGGRAAATALGVIGGALLGNQIESGRP
                     GYQNVQRCTTETYYDQRVIGYDVTYEYAGRSYTTRTQSDPGQWIPITVQPAVPGMSGY
                     TPPGYAAQNAGDPGYGHPGAVYGTPPGPPAYVVPPPAVYEHRYPYYGHYPYRQPRYNY
                     "
     gene            8702..9019
                     /locus_tag="PK518_11325"
     CDS             8702..9019
                     /locus_tag="PK518_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRM49549.1"
                     /translation="MTTESIAGATVSTQANVYFDGKCVSHSLTLADGTKKSVGVVLPS
                     TLTFNTGAAEVMECVAGGCEYKLAGSDAWQTSGPGDKFSIPANSSFDIRVDQAYHYIC
                     HFA"
     gene            9084..>9538
                     /locus_tag="PK518_11330"
     CDS             9084..>9538
                     /locus_tag="PK518_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRM49550.1"
                     /translation="MATILQSLPVGQKVGIAFSGGLDTSAALRWMKNKGALPYAYTAN
                     LGQPDEDDYDAIPRKAMEYGAEKARLIDCRTQLAHEGIAAIQAGAFHISTGGISYFNT
                     TPLGRAVTGTMLVAAMKEDDVHIWGDGSTFKGNDIERFYRYGLLTNPSL"
BASE COUNT         1608 a         3275 c         3065 g         1590 t
ORIGIN      
        1 cgcgaccccc ggcgtgacag gccggtattc taaccgactg aactaccact ccgcgtcggt
       61 agctgcgttg cgactcttgc gagtccaagt gcagccaaac ttggcgaccc tacggggatt
      121 cgaaccccgg tactcaccgt gaaagggtga tgtcctaggc ctctagacga tagggtcaaa
      181 acctagattc agtgtggtgg aggtaaacgg gatcgaaccg atgacctctt gcatgccatg
      241 caagcgctct cccagctgag ctataccccc actcagattt cagaaacttt cgtatccgtc
      301 atctgctgag ccttgaatta tagacaggtt tttaacgact tttcaaacgt gtgaggattt
      361 tttcgcgccc caggagttcc agcaccgcat cgaccgacgg cgtgtgtgcc tggcccacgg
      421 tcagcacgcg cacgggcatg gccagttgcg gcatcttcgc gccctgctcc ttcagcagtt
      481 gcttgatggc ggcggcgatc gcctccttgt tccactccac cgtggccatc gctgtggcaa
      541 aggcgtccag cagcggaccg gcctcgggcg tgacatgcct ggcatagtct tcggcgttgc
      601 gctccacgcg gtccagatag aacacacgta cccagcgtgc cagatcgacc agggtctcgc
      661 agcgatcctt gaacagcgcg cagatgcgcg gcagacgctc gtccagcgcc gccggggcca
      721 cgccttcgtt ctcgacgaag ggggccacca ggcgcgcgag ctcaaagtcg tcgagtgcct
      781 tcatatgctg ggcgttgacc cagcgcagct tggcctcgtc gaactggccg gcgctacggc
      841 ccaggtggtc gaggttgaac cactccagga actgcgcgcg cgagaagatt tcgtcgtcgc
      901 cgtggctcca gcccaggcgc gccaggtagt tgaccatggc gtcgggcagg tagccctcgt
      961 cgcggtactg cgtgacagcc ttggcgccat tgcgcttgct catcttctcg ccctgctcgt
     1021 tcaagacggt gggcaagtgg gcaaacaccg gcacctgcgc tccaagtgcc tcgaagatgt
     1081 ggatctggcg cggcgtgttg ttcacatggt cgtcgccgcg gatcacatgg gtgatgcgca
     1141 tgtcgatgtc atcgacacag acgcagaagt tgtaggtggg cgtgcccacg gcttcgccgt
     1201 tctgtgccgg gcgggcgatg accaggtcgt cgagctcgct attttgaaat tcgatgcggc
     1261 ccttgcactt gtcatcccat gccaccacgc catccttggg cgtcttgaag cgcagcaccg
     1321 gctgtacgcc ctcgggcacg ggcggcagct gcttgccagg ctcggggcgc caggtaccgt
     1381 cgtagcgcgg cttttccttg gcggcggtct ggcgctcgcg cagcgcgtcc agctcggcca
     1441 tgctcatata gcaggggtag acatggccgc tggcctgcag ctggaccagc acttccctgt
     1501 aacgcgccat gcgctgcatc tggtagtacg ggccttcatc gtgatccagc cgcagccagg
     1561 ccatgccctc caggatcaca tcgacgctgg cctgggtgga gcgctcgacg tcggtgtcct
     1621 cgatgcgcag gatgaagtcg ccacccatgg cgcgcgcaaa ggcccagggg tagagcgccg
     1681 agcggatgtt gcccaggtgg atgaagcccg tcggcgacgg ggcgaaacgc gtgcgtactt
     1741 ttgcttgtgt catgccagag tgtccaggcc gcgcaagagg tcggccttca ggtcgtcgag
     1801 atgttccagg cccacggaga tgcgcaccag cccctggcct atgccagcgg cctggcgctg
     1861 ttcttccgtc aggcgcccgt gcgaggtgct ggccgggtgg gtgatggtgg tcttcacgtc
     1921 gcccaggttg gcggtgatgg agcacacgcg cgtgctgtca atcacgtgga aggcgttggc
     1981 gcgcagctgc tcggcgccct gccccgccac gtcgaatgcc agcaccgtgc cacccatgcc
     2041 gttttgctgg cgcatggcca gctcatgctg cgggtggctc ttcaggccgg ggtagtagac
     2101 gcgcgccacc ttgggctgct gctccagcca ggcggccagt tccagcgcgg cggcgctctc
     2161 ggccttgacg cgcagggcca gggtctccag acccttcaag accacccagg cattgaacgg
     2221 tgccaggttc agcccgccgc tgcgcaaaaa ggtgcccatg accttgtcca ccagtgccac
     2281 cgtgccgcac acggcgccgg ccatgacgcg gccctggccg tcgagcagct tggtgcccga
     2341 gtgcacgacg atgtcggcgc caaacctggc cggctgctgc agcacgggcg aggcaaaact
     2401 gttgtccacc gccagcaacg cgccgtgggc gtgggcaatt tcagccagcg cggcgatgtc
     2461 gcacaggtcg gtgagcgggt tggtgggcgt ttcggcgaac agcaagcgcg tgttcgggcg
     2521 gatcgcagcc tgccaggccg ccacatcggt ctgcgagaca aagctggtct ccacgccaaa
     2581 gcgcgccatc tcggtgccca gcagcttgat ggtggagccg aacatggact gcgagcagac
     2641 cacatggtcg ccggccttga ggaccgtgag cgccaccagc aggatggccg acatgccggt
     2701 gctggtggcc acggcgcatt cggtgccctc catggcggcc aggcggcgct cgaaactggt
     2761 gacggtggga ttggccgtgc ggctgtaggt gtagcccgcc tcctcattgg caaagcggcg
     2821 cgcggagctg gcgcagtcgc tctgcacgaa gctgctgctc aggtacaggg cttcgctgtg
     2881 ctcgccccac tggctgcgct ccacggcctc acgcacggcc agggtgtcgg ggtgcaggcc
     2941 cgggggcagg gatttctcgg tcacggtgac aatccttatt caagaatagg agctgcgtgc
     3001 gcttgttgca cggtggtttg aatcaacttc tcttatgaaa ccgcccattg acaggcgcaa
     3061 ggcgctcact tttttccgtc tgcgccggca ggctactcct gggcgttggg cagcgacagg
     3121 cgcgaggtgt cttcctcgtc atcctcgccg ccgcgctggc gcccctcgtt cagggccgtc
     3181 acttcctcgt cggagatgtc gccggtgatg tacaggccgt cgaagcagga ggcctcgaag
     3241 ccctgcacgc ccgggttgat cttgcccacg gcctgcttca tggccgccac gtcctggtag
     3301 atcagcgcgt cggcgcctat cacctggcgg atttcttcct cggtgcggtc gtgcgccacc
     3361 agttcggcgc gcgtgggcat gtcgatgccg tagacgttgg ggtggcgcac cggcggagcg
     3421 gccgaggcca gatacacctt gaccgcgccg gcgtcgcgcg ccatctgcac gatctccttg
     3481 gaggtggtgc cgcgcacgat ggagtcgtcc accaacagca cgcgcttgcc cttgaactcg
     3541 ctggcgatgg cgttcaactt ctggcgcacc gacttcttgc gcgtgccctg gcccggcatg
     3601 atgaaggtgc ggccgacata gcggttcttc acaaaacctt cgcggtaggg caggcccaag
     3661 agctgcgcca gctgcatggc gctggggcgg ctggattcgg ggatggggat gatggcgtcg
     3721 atctggctcg gtggcacggt ggagaccaca cgcttggcca gcgtctcgcc catgttcagg
     3781 cgcgcctggt agaccgagat gccgtccatg atcgagtccg gacgtgccag gtagacatac
     3841 tcgaacacac agggatagag gcgcggttgc tgcgcgcact gccgggtttg cacggtgccg
     3901 tccaactgca cgaatacggc ctcacccggc gcgatgtcgc gctcgagccg gtgcaacgtg
     3961 ccttccaggg ccaccgactc gctggccagc atcaccgtgc cgtcagcgcc ccggcccatg
     4021 cacagcggac ggatgccgaa cggatcgcga aaggccagca ggccatagcc ggcaatcagt
     4081 gccaccaccg catacgagcc cttgatgcgc tggtgcacgg cgcgcacggc ggcaaacacg
     4141 tcgtccgtgg tcagcgatgc gccgctgctg gcgcgcgcca actcgtgggc cagcacgttg
     4201 agcagcacct cggagtcgct ctcggtgttg gtatggcggt ggtcagtctc ggccagctcg
     4261 cggcgcagct gcttggcgtt ggtcaagttg ccgttgtgca ccatgacgat gccgaacggc
     4321 gcgttcacgt agaacggctg cgcctcctcc tcgctggccg cactgcccgc cgtcgggtag
     4381 cgcacctggc ccagacctat gctgccgggc agcgcgcgca tattgcgcgt gcggaacacg
     4441 tcgcgcacca tgcccttggc cttgtgcatg aagaacttgc gcccctcctg ggtgactatg
     4501 ccagcggcat cctgcccccg gtgctgcagc agcagcaagg cgtcatagat cagctgattc
     4561 acaggggcgg tgctgacgac gccgacgatt ccacacatag ttcgcgttcc attttcaatg
     4621 gcgggagact tcccgccgac aaccattgac tgcccactgg cagcagcgcc ctcgctacaa
     4681 cggcagatgc cgccccagct cctcgggcag cacgggccgc atgcccttga gcgccacctg
     4741 cagccagggc gccagctgcg actgccgcca ccagggcgca tcttgcacct gcagccagcc
     4801 ggcgaccagc gccacgacca gcagcagcag caggccacgc agcacgccaa acagcgcccc
     4861 cagcgcccgg tcggccggcc gcaggccgac ggcatcgaca agcttcttga gcagccaggc
     4921 cagcaggccg caggcaaaca ccgcggcaac aaacaccacc acaaaaccgg ccacatggcg
     4981 ccatgcggca tccgcctcgc ccatgggcaa ccaggccccg gcggcatcgg caaaccactg
     5041 cgcagcaaag aatgcaacga cccagccagc cagcgacaac aattcgaaca ccaggccgcg
     5101 ccacgcgcca atcaacagcg acgcggccag caccgtcaga aacacccagt ccagcgctgc
     5161 catcgccatc catccagagc cggtggcttg ttacaacgcc agcacggaag ccgacaggcc
     5221 agcgctcttg acgcgcccaa ccgccttgtc ggcctcggca cggctgtcaa acggccccag
     5281 acgcacgcgc gtgcgcttgc cgtccttggt gtccaccacc tgggtatagg tcttgaggcc
     5341 cgagcgttcc agcttctcgc gcacctcgcg caccttgtcg gcatcggcaa aggcacccac
     5401 ctggacgatg aaacggccct gatcggccgc agccgcgctg gcggcctgcg ccggctgctg
     5461 gccccgacct tcgagcaggg cccgggcgcg ggccgcgtca tccgggcgtg ccggcttggc
     5521 gtcgggcttg ggttcgggct tgtgctccac cttgggctcg ggtttcggct cggctttcgg
     5581 ttccggccgg ggagcgggct tgggctcagt cctggcttca ggtttggcct cgacctgcgc
     5641 ggcgggttct gcgcgactgc tggtaccgat cacttcctcg ccagcgtcca acccggccgc
     5701 agccggctct ggcttggccc tgggctcgtc ctggctggcg cccttgcctg gtaccgccag
     5761 cggggcgacc ttgttgcgat cgggaatgtc gatggccacg tccaccgcca ccgggcgcgg
     5821 ctgggtgtcg aaaaccagcg gaaagccgac cacgcccgcc agcaccagca cggccgcgcc
     5881 gatcaggcga tggcgcgccc ggcggcgcat gtcgtcgatg ctctcggcct gtgcggctgc
     5941 gcgcgagcgc cgtgcgccct tgttgctgtc ggcctgcggc tcggcaccgc cgggccaacg
     6001 aaacttgaaa aatgccatgg agtgtggact ggttaggcgt ccagagttag gcgtccagat
     6061 gcttggcgtg cagtcggggc actccgtttt tgagcacgcc gcccaccgtg tagaacgaac
     6121 caaagaccac aattctatca gtggggtcgg ccgcctcgac ggccgcacgc agcgcctctt
     6181 cgggactggc gtgcaggctg gttccagcct tgcggccggt ggccgaagcc gctggcaatg
     6241 tactccactt ctgctgcagg ccggccgcgc tctcggcgcg cggcgtgggc aggtcggtga
     6301 aataccagcg gtcgatgagg ggggcgatct tctccagcat gggcgccaga tccttgtcgg
     6361 ccatggcgcc aaacacggcg tgcgtggtcg gaaagtagcc catggcgtcc aggttcgccg
     6421 tcagcgccgc caccgaatgc gggttgtgcg ccacatccag caccagggcc ggctggccgg
     6481 gcacgacctg gaagcgcccg ggcagctcca cccgcgccag ccccaggcgc accgcctgcg
     6541 ccgtgaccgg caggcgctgg cgcagcgcct ccagcgctgc cagcgcgccc gaggcgttga
     6601 tgagctgatt ggccccgcgc agcgccggat aggccaggcc ggcataacgc cgcccgcgcc
     6661 cggcccagga ccattgctgc ttgtcgcccg aatagttgaa gtcctgcccg aagcgccaca
     6721 ggtcggcgcc gatctgctgc gcctgatcga tcacgctttg cggcggcaac gggtcgctga
     6781 ccaccaccgg tcggccggca cgcatgatgc cggccttctc gcgcccgatc gattcacggt
     6841 ctgacccgag aaattccaca tggtcgatgt ccacgctggt gatcacggcg cagtcggcgt
     6901 cgatgatgtt ggtggcgtcc agccgcccgc ccaggccgac ctccagaatg gccacatcca
     6961 ggcccgcctg gctcatgaac cgcaggatgg ccagagtggt gaactcgaag taggtcagcg
     7021 acacctcggt cccggcttcc gtccgggcgc gctccacggc ctcgaaatgc ggcagcagcg
     7081 ctgcggcagc gacgctgtcg ccgcgcacgc ggcagcgctc ctcgaaatgc accagatgcg
     7141 gcgatgaata caccccggtg cggtagccgg cctccatggc cacggcctcc agcagggcgc
     7201 aggtggagcc cttgccgttg gtgccggcga cggtgatcac cgggcagtca aagcgcaacg
     7261 ccagccggcg cgccacctcg cccacgcggg ccaggcccat gtcgatattc ttggcatgca
     7321 gccgctcgca atgctcgagc cagccttgca gtgtctgggg ataggtttgc atagagcccg
     7381 cgattgtcgc cgagccggcc atgggcgatg atgcgcgccc atgagtaccc ccaccacgat
     7441 catcctctac ggcattccca attgcgacac ggtgaagaag gcgcgcgcct ggctcgccgg
     7501 gcagagcctg gcctaccagt tccacgactt caagaagcag ggcgtgcccg cggaacgcct
     7561 gagcgcctgg atggccgccg tgggctggga caggctgctc aaccgccagg gcacgagctg
     7621 gcgcaggctg gacgctgcag tgcagtccag cgtgcaggac gccgccagtg ccgccgcgct
     7681 gatgcgcgaa catgcctcgg tcatcaaacg cccggtggtc gagtggaacg gcacagccac
     7741 gggccgcgtg accgtgggct tcaagcccga gacctggaac gaattgctgg ccgccggctc
     7801 caaaacatag gtctttacgc cagatttacc tggccgccat ggcagaaaag cacacccggg
     7861 tgaaactgcc tgcgaccctg gtgcgttaca ggctgaccga aaggagcttt cagtccatga
     7921 acaagatcat cgtccttgcc accctcacca tggcggccat ggccagccag gcccaggatc
     7981 tgggccgcgt gctgtcggcc acccccatca cccagcaggt ggccgtgccg cagcaggtgt
     8041 gcggcaacga gaccatttac aacggggcgc gcccgacgac cggcggtggc gcagtgctgg
     8101 gcgccattgc cggtggcgcc gcgggcaatg ccattggcag cggcggcggc cgcgccgcag
     8161 ccacggccct gggcgtgatt ggcggcgcgc tgctgggcaa ccagatcgag agcggccgcc
     8221 ccggctacca gaacgtgcag cgctgcacca ccgagaccta ctacgaccag cgcgtgatcg
     8281 gctacgacgt gacctacgaa tacgccggcc gcagctacac cacacggacc cagtccgatc
     8341 ccgggcagtg gattccgatt accgtgcagc cggccgtgcc gggcatgtcc ggctacacgc
     8401 cgcccggcta cgcagcgcag aacgccggcg atcccggcta cggtcacccc ggcgcggtct
     8461 acggcacccc gccgggcccg ccggcctatg tggtgccgcc accggcggtc tacgagcacc
     8521 gctaccccta ttacggccac tacccctatc gccagccgcg ctacaactac tgaagatccg
     8581 cagccaccac aggagccctc cagggctcct tttttcatgg cgcagcgcac gccagggggg
     8641 cagcggcgcg cgcggccggc ctagaattgc cggtttgtcc acccctcttc agtcaccacc
     8701 catgaccacc gaatcgattg ctggcgccac ggtcagcacc caggccaacg tgtactttga
     8761 cggcaaatgc gtgagccaca gcctgacgct ggccgatggc acgaagaagt cggtgggcgt
     8821 ggtattgccg tccacgctca ccttcaacac cggcgcggcc gaggtcatgg aatgcgtagc
     8881 cggcggctgc gaatacaagc tggccggcag cgatgcctgg cagacatccg gccctggcga
     8941 caagttcagc atccccgcca actccagctt cgacatccgg gtcgatcagg cctatcacta
     9001 catctgccat ttcgcatgag catttttagc ctgtagcgct tatctataaa gcgctaacag
     9061 ctatcatttt tgagagaacc tccatggcaa ccattttgca atccctgccc gtcggccaaa
     9121 aggtcggcat cgccttctcc ggcggcctcg acaccagcgc cgcgctgcgc tggatgaaga
     9181 acaagggcgc cctgccctac gcctacaccg ccaacctggg ccagcccgac gaggacgact
     9241 acgacgccat cccgcgcaag gccatggaat acggcgccga gaaggcgcgc ctgatcgact
     9301 gccgcaccca gctggcgcac gagggcattg ccgccatcca ggccggggcg tttcacatct
     9361 ctacaggcgg catcagctac ttcaacacca cgccgctggg ccgcgccgtc acgggcacca
     9421 tgctggtcgc cgcgatgaag gaggacgatg tgcacatctg gggcgatggc tccaccttca
     9481 agggcaacga catcgagcgc ttttaccgct acggcctgct gaccaacccc tcgctcaa
//
LOCUS       DAOMTQ010000118         9298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1500_length_9298_cov_26.357352, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000118 DAOMTQ010000000
VERSION     DAOMTQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1500_length_9298_cov_26.357352"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PK518_11335"
     CDS             <1..838
                     /locus_tag="PK518_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HRM49551.1"
                     /translation="ISAGAHVRAMQTSARMLRAGQEVREYHLEAELLHEFRRHGSQYP
                     AYGSIVAAGANACVLHYRADTAPVRAGELVLIDAGCELDGYASDITRTFPADGRFSGP
                     QRALYDLVLASEKAAIAATRAGARFTDPHDAAVAVLAQGMLDVGLLDKNKVGSAQDVI
                     EKRAYFQFYMHRTGHWMGMDVHDCGSYVEPGEVGQVSERKDPLSGELILNRPSRILRA
                     GMVLTIEPGIYVRPAPGVPEAFHHIGIRIEDDALVTDAGCELMTRGVPVEPDEIEALM
                     RA"
     gene            complement(1142..>1909)
                     /locus_tag="PK518_11340"
     CDS             complement(1142..>1909)
                     /locus_tag="PK518_11340"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HRM49552.1"
                     /translation="KTWFATAFGAAAIAMAGPAAAVPVAVELALLVDVSGSVDNSEYN
                     LQKTGYVNAFKDAIIQSNIASLTGGIAVTYIEWSGANQQSSLVNWTHVTDAASANAFA
                     DAIAATTRAFASLTAPGSAINYVAPGFSANSYEGARWVIDVSGDGPQNDGANTATARD
                     NFLNSAPTGVSAVVNGLAILGEFGLQDWYQNNIQGGANAFVIAANGFADFDNAVKTKI
                     GKEIHDGGGNVPEPATLALVGLALLGAGAARRKGSRN"
     assembly_gap    1910..2009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2397..3806)
                     /locus_tag="PK518_11345"
     CDS             complement(2397..3806)
                     /locus_tag="PK518_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HRM49553.1"
                     /translation="MRKTFMGVKLRALRAERGLSQTALAQALELSPSYLNQIEQNQRP
                     LTVPVLLKLHRALGVDIQQFSEDEEARLIAGLREALADAAEPIALPELQEVAAQMPAL
                     GRALIALHRRRLQADERLETLALGLGDDRGQAVAARAMPFEVVRDFFFDHQNHFDAVD
                     RAAETLAAQAPGAQGGQLGPWLTERLRQRHGVQLVLAGDGSGAKRSYDPTQRRLLLSP
                     ELEPAQQAFQLATQLALLELPALMDQLTDAPPFSHDEAARSLARIGLANYFAGALLLP
                     YGAFLQAAEALRYDIGLLQQRFGVGFETVCHRLSTLQRPGAPGVPFFFVRVDRAGNIS
                     KRQSATQFHFSRTGGTCPLWNVYEAFEQPGRVLRQLASMPDGRKYLWIARTVSGGGRG
                     YGAPRKTFAVALGCDMRHAPRLVYAQGLDLNAAATPIGMGCKVCERAQCPQRAFPYMG
                     AALAVNEHQSRLVPYGFAP"
     gene            3958..5121
                     /gene="prpC"
                     /locus_tag="PK518_11350"
     CDS             3958..5121
                     /gene="prpC"
                     /locus_tag="PK518_11350"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HRM49554.1"
                     /translation="MNTAIADAPAAPKAKKSVALSGVQAGNTALCTVGRTGNDLHYRG
                     YDILDMAEVCAFEEIAHLLVHGKLPTRAELASYKAKLRALRGLPGSVKAALEQLPAAS
                     HPMDVLRTGVSALGCALPEKDDHNLAGSRDIADRLLASLGSMLLYWYHFSNSGRRIEV
                     QTDDESIGGHFLHLLHGQKPQEAWVRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMY
                     SSIAGAIGALRGPKHGGANEVAFEVQKRYDSPNAAEADIRQRVANKEVVIGFGHPVYT
                     VSDPRNVVIKGVAKQLSQAAGSTKMYDIAERLEVVMWEEKKMFPNLDWFSAVSYHMMQ
                     VPTAMFTPLFVIARTSGWAAHVIEQRQDNKIIRPSANYTGPEDQRFVPLAERF"
     gene            5233..7869
                     /gene="acnD"
                     /locus_tag="PK518_11355"
     CDS             5233..7869
                     /gene="acnD"
                     /locus_tag="PK518_11355"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HRM49555.1"
                     /translation="MNSTHRKPLPGTALDYFDARAAVEALTPGAWARLPYSARVHAEN
                     LLRRADPAQLNDYLRQLVERRRDIDFPWYPVRVVCHDILGQTALVDLAGLRDAIAQEG
                     GDPAQVNPVVPVQLIVDHSLAVECGGFDPEAFAKNRAIEERRNADRFHFIEWCKQAFA
                     NVQVIPAGNGIMHQINLEKMSPVVASQDGVAYPDTCVGTDSHTPHVNALGVLAIGVGG
                     LEAENVMLGRASWMRLPEVVGVELSGQPASGITATDIALALTEFLRREKVVGAYVEFF
                     GAGAQSLSVGDRATISNMCPEYGATAALFHIDQQTLDYLRLTDRSPEQVQLVEAYAKA
                     AGFWADGLQAAEYERVLRFDLSSVVRNMAGPSNPHRRLPVSALAERGIADAAQLAAAR
                     ADEAQGLLPDGAVIIAAITSCTNTSNPRNVIASALLARNARRLGLSRKPWVKTSLAPG
                     SKAVELYLQEAGLLEDLEALGFGIVGFACTTCNGMSGALDPAIQQEITERGLYTTAVL
                     SGNRNFDGRIHPHANQAFLASPPLVVAYALAGTVRFDIENDVFAVVDGKEVRLHDLWP
                     SDAEIDAVVARAVQPAQYRAVYQPMFSLRKSAAAPAAPQYAWRAQSTYIRRPPYWDTE
                     GVGALAAFPRTLTNLRPLALLGDNITTDHLSPSNAILADSAAGEYLAKMGLPEEDFNS
                     YATHRGDHLTALRATFANPQLVNEMAVVGGQVQKGSLARIEPEGQVVRMWEAIETYLQ
                     RRQPLIIIAGADYGQGSSRDWAAKGVRLAGVEVVAAEGFERIHRTNLIGMGVLPLQFA
                     PGVNRSTLGLDGSETYEVQGRLTPGATLTLLVQRHNGQRLQVPMACRLDTAEEVSVYE
                     AGGVLQRFAQDFLAKHASDALV"
     gene            7891..9069
                     /gene="prpF"
                     /locus_tag="PK518_11360"
     CDS             7891..9069
                     /gene="prpF"
                     /locus_tag="PK518_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HRM49556.1"
                     /translation="MSKSAQLRIPATYMRGGTSKGVFFHLPDLPESARQPGAARDALL
                     LRVLGSPDPYGKQIDGLGNASSSTSKAVILSRSQRPGHDVDYLFGQVAIDRPLVDWSG
                     NCGNLSAAVGPCAIHMGLIAAARIPENGLCTLRIWQANIGKTIVAHVPITHGQVQETG
                     DFELDGVAFPAAEVALEFMAPAAEDGGMFPTGQVLDMLDVPGVGSFQATLINAGIPTV
                     FLNAQDLGYTGTELQRDINQDPQALARLEAIRAWAALQMGLIADLAEAAQRQHTPKIA
                     FVAPPAGYATSSGRRVEAGAVDLLVRALSMGQLHHAMMGTAAVAIGTAAAVPGTLVNL
                     AAGGGTRNTVCFGHPSGSLRVGAEVARVDGQWQVTKAAMSRSARILMEGMVRIPAGSF
                     "
BASE COUNT         1635 a         3090 c         2967 g         1506 t
ORIGIN      
        1 catcagcgcc ggcgcccatg tgcgcgccat gcagaccagt gcccgcatgc tgcgcgccgg
       61 ccaggaggtg cgcgaatacc acctggaggc cgaactgttg cacgagttcc gccgccatgg
      121 ctctcagtac ccggcctatg gctccatcgt cgccgccggc gccaacgcct gcgtgctgca
      181 ctaccgtgcc gacaccgcac ccgtgcgcgc cggtgagctg gtgctgatcg acgccggctg
      241 cgagctcgat ggctatgcgt cagacatcac gcgcaccttc ccggccgatg gacgcttcag
      301 cgggccgcag cgcgcgctct acgacctggt gctggccagc gagaaggcgg caatagccgc
      361 cacgcgcgcc ggggcgcgct ttaccgaccc gcatgacgcg gccgtggccg tgctggccca
      421 gggcatgctg gatgtcggac tgctggacaa gaacaaggtc ggcagcgcac aggacgtgat
      481 cgaaaagcgc gcctacttcc agttctacat gcaccgcacg ggccactgga tgggcatgga
      541 cgtgcatgac tgcggcagct atgtggagcc cggcgaggtc ggccaggtga gcgagcgcaa
      601 ggatccgctg tcgggcgagc tcatcctgaa ccgccccagc cgcatcctgc gcgccggcat
      661 ggtgctgacc atagagcccg gtatctacgt gcgcccggcg cccggcgtgc ccgaggcttt
      721 ccaccatatc ggcatacgca tagaggacga cgccctcgtg acggacgcgg gctgcgagct
      781 gatgacgcgc ggcgtgccgg tcgagccgga tgaaatcgaa gccctgatgc gcgcctgact
      841 cagaagccca gaccctgccc taaggcgcaa agctgcgggg tgcaccgcaa ctgtgccttt
      901 cactgcacct gcagcggcag gcttgtgtat ggaaagatgc actgcgggct ggtttggtgc
      961 ccgcagggct tgcagccacg ctcacgggcg tggttgcagc gttcagggat aagcggccga
     1021 tgctcggctt gcgcctcccg gacggcatca gggctatcgc catagatcgg tggcacccat
     1081 gaaaataaaa cgggccgatg actcggcccg ttgctgtcgc cacttctgac cgccaaaggt
     1141 gtcagttcct ggatcccttg cgacgtgcag caccagcgcc cagcagcgcc aagcccacca
     1201 gtgcgagtgt ggcaggttct ggaacgttgc cgccgccgtc atggatctcc tttccgatct
     1261 tggtctttac tgcgttgtcg aaatctgcaa aaccgttcgc tgcgatcaca aaggcgttgg
     1321 caccaccctg gatgttgttt tggtaccagt cctgcagacc gaactcgcca aggatcgcca
     1381 agccgttgac gaccgctgac acccccgtgg gggcgctgtt gaggaagttg tcacgggcgg
     1441 tggcggtgtt ggcaccatcg ttttggggac catcgccaga aacgtcgatc acccagcgtg
     1501 cgccttcgta ggaattggcc gaaaagccag gagcgacata gttgatcgcg ctgcccggag
     1561 cagtcaaact tgcgaaggcg cgcgtagtgg cagcaattgc gtccgcgaag gcatttgcgc
     1621 tggcggcgtc ggtgacatgt gtccaattga ccagggagga ttgctggttt gcgcctgacc
     1681 actcgatgta ggtcaccgcg atgccgccgg tcaggctggc gatgttggac tgaatgatgg
     1741 cgtccttgaa cgcgttcaca taacccgttt tttgcaggtt gtactcagag ttgtcgacgc
     1801 ttccggaaac atcgaccagc aaggccaatt ccactgctac cggcacagcc gctgccgggc
     1861 ctgccattgc gattgctgct gcgccaaagg cggtggcaaa ccaagttttn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgggcctgcc attgcgattg ctgctgcgcc
     2041 aaaggcggtg gcaaaccaag ttttggtttt cataggattc tcctatcaat gctgttgcgg
     2101 cacataccag cgctgatgga gcaaaaataa tgccatattt ataagtgttt gatatttaag
     2161 aaaaatatta cctccttgaa gaagatcgca aactttgtgt aaagtttatc gacactttta
     2221 ggtaggacga tccatttttg ctgagactgt ttaatgcacg ccgagcgtgc gcgagcagcc
     2281 cccctcgaca tccgcatcga agacgacacc atcgtcaccg agtccgactg cgggtccata
     2341 gcgtgcggcg tgtccgtggc ggtcgatgag atcgaagcgc taatgcgtca ataaggctac
     2401 ggcgcaaagc cgtagggcac cagccggctc tggtgctcgt tgaccgccag cgccgcgccc
     2461 atgtacggaa acgcgcgctg cgggcattgc gcgcgctcgc agaccttgca gcccatgccg
     2521 ataggcgtgg cggcggcgtt cagatccagc ccctgggcat agaccaggcg cggcgcgtgg
     2581 cgcatgtcgc agcccagggc gacggcgaag gtcttgcgcg gcgcgccata gccacgtccg
     2641 ccgcccgaga ccgtgcgcgc aatccacagg tatttgcgcc cgtcgggcat gctggccagc
     2701 tggcgcagca cgcggccggg ctgctcgaag gcctcgtaca cattccacag cgggcaggtg
     2761 ccgccggtgc ggctgaagtg gaactgcgtg gccgactggc gcttgctgat gttgcccgcc
     2821 cgatccacgc gcacgaagaa gaacggcacg cccggcgcgc ccgggcgctg cagcgtggac
     2881 aggcggtggc acacggtctc gaagcccacg ccaaagcgtt gctgcagcag ccctatgtcg
     2941 tagcgcagcg cctcggccgc ctgcaaaaag gcgccgtagg gcagcagcag cgcgccggca
     3001 aagtagttcg ccaggcctat gcgtgccagg ctgcgcgcgg cctcgtcatg gctgaacggc
     3061 ggggcatcgg tgagctgatc catcagcgcc ggcagctcca gcagcgccag ctgcgtggcc
     3121 agctggaatg cctgttgcgc cggctccagc tcgggcgaca gcagcagccg gcgttgggtc
     3181 gggtcgtagc tgcgcttggc gccgctgccg tcgcccgcca acaccagctg cacgccatgg
     3241 cgctggcgca ggcgctcggt cagccatggg cccagctggc cgccctgcgc gcccggcgcc
     3301 tgggcggcca gggtttcggc ggcgcggtcc acggcgtcga aatggttctg gtggtcaaag
     3361 aagaagtcgc gcaccacctc gaaaggcatg gcgcgcgcgg ccacggcctg gccgcggtcg
     3421 tcgcccaggc ccagcgccag cgtctccagc cgctcatcgg cctgcagccg gcgccggtgc
     3481 agggcgatca gcgcccgccc cagcgccggc atctgcgccg ccacctcctg cagctcgggc
     3541 agggcgatgg gctcggcggc gtcggccagg gcctcgcgca ggccggcgat caggcgcgcc
     3601 tcctcgtcct cggagaactg ctgtatgtcc acgcccaggg cgcggtgcag cttcagcagc
     3661 accggcacgg tgagcgggcg ctggttctgc tcgatctggt tcaggtagct gggcgagagt
     3721 tccagcgcct gggccagcgc ggtctgcgac aggccgcgct cggcacgcag cgcgcgcagc
     3781 ttcacgccca tgaaggtctt tctcatgcgc gccctccgcg gctctgggga ttcgcaaaat
     3841 ttgcaatttg tcggtgtgtg attcgcaaga cttcgccttt tcagccctgt tggtatgggg
     3901 gtattttgcg taaattgcga agcatcgcaa gcgcatcagc aacaaggagc cccgcacatg
     3961 aacaccgcca tcgccgacgc accggccgcg cccaaggcca aaaaatccgt cgccctctcg
     4021 ggcgtgcagg cgggcaatac cgccctgtgc accgtgggcc gcacgggcaa tgatctgcac
     4081 taccgcggct acgacatcct ggacatggcc gaggtctgcg catttgaaga aatcgcccac
     4141 ctgttggtgc acggcaagct gcccacgcgc gccgaacttg ccagctacaa agccaagctc
     4201 cgcgccctgc gcggcctgcc cggcagcgtg aaggccgcgc tggagcagtt gcccgccgcc
     4261 agccacccca tggatgtgct gcgcaccggc gtctccgccc tgggctgcgc cctacccgag
     4321 aaggacgacc acaacctggc gggcagccgc gacattgccg accgcctgct ggccagcctg
     4381 ggcagcatgc tgctgtactg gtaccacttc agtaactcgg gtaggcgcat cgaggtgcag
     4441 accgacgatg aatccattgg cgggcatttt ctgcacctgc tacacggcca aaaaccgcaa
     4501 gaggcctggg tgcgcgccat gcacacgtcc ctgaacctgt acgccgagca cgagttcaac
     4561 gccagcacct ttaccgcacg cgtgattgcc ggcacgggca gcgatatgta ctccagcatc
     4621 gccggcgcca taggcgcgct gcgcggcccc aagcatggcg gtgccaacga ggtggcgttt
     4681 gaggtgcaaa agcgctacga cagccccaac gcggccgaag ccgacatccg tcagcgcgtt
     4741 gccaacaagg aggtggtgat cggctttggc cacccggtct acaccgtgag cgacccgcgc
     4801 aacgtcgtca tcaagggcgt ggctaagcag ctgtcgcagg cggctggttc gaccaagatg
     4861 tacgacattg ccgagcgcct ggaggtggtg atgtgggagg agaaaaaaat gttccccaac
     4921 ttggactggt tcagcgccgt cagctaccac atgatgcagg tacccacggc catgttcacg
     4981 ccgctgttcg tcatcgcccg caccagcggc tgggcggcgc atgtgatcga gcagcgccag
     5041 gacaacaaaa tcatccgccc cagcgccaac tacaccgggc cggaagatca gcggtttgtc
     5101 cccctggccg agcgtttcta ataaaaaaat aatagctatt aatgcttgat acgagacgtt
     5161 ttaatattga tttgatattg aaaatggctt aaataaagcg ttagcagata tcaaataaat
     5221 agtaaaccca cgatgaactc cacccaccgc aaacccctgc ccggcacagc gctggactat
     5281 ttcgacgccc gcgccgccgt cgaagccctc acccccggtg cctgggcccg cctgccctac
     5341 agcgcccgcg tgcatgccga aaacctgctg cgccgtgccg atccagcgca gctgaacgac
     5401 tacctgcgcc agctcgtaga gcgccggcgc gacatcgact tcccctggta cccggtgcgc
     5461 gtggtgtgcc acgacatcct gggccagacg gcgctggtgg acctggcggg gctgcgcgat
     5521 gcgatcgcac aagaaggcgg cgacccggcg caggtgaatc cggtggtgcc ggtgcagctc
     5581 atcgtggacc attcgctggc cgtggagtgc ggcggctttg accccgaggc gtttgccaaa
     5641 aaccgcgcca tcgaggagcg gcgcaacgcg gatcgctttc acttcatcga gtggtgcaaa
     5701 caggcctttg ccaatgtgca ggtgatcccg gcgggcaacg gcatcatgca ccagatcaat
     5761 ctggagaaga tgtcgcccgt ggtcgccagc caggacggcg tggcctaccc cgacacctgc
     5821 gtgggtaccg acagccacac cccgcacgtc aacgccctgg gcgtgctggc cataggcgtg
     5881 ggcgggctgg aggccgagaa cgtgatgctg ggccgcgcca gctggatgcg cctgcccgag
     5941 gtggtgggcg tggagctctc gggccagccc gccagcggca tcaccgccac cgacatcgcc
     6001 ctggcgctga ccgaattttt gcgccgcgaa aaggtggtgg gcgcgtatgt cgagttcttt
     6061 ggcgcggggg cgcaaagcct gtcggtgggc gaccgcgcca ccatcagcaa tatgtgcccg
     6121 gagtacggcg ccacggccgc gctgttccat atcgaccagc agaccctgga ctacctgcgc
     6181 ctgaccgacc gcagcccaga gcaggtgcag ttggtcgagg cctacgccaa ggccgcgggc
     6241 ttctgggccg atgggctgca agccgccgag tacgagcgcg tgctgcgctt tgacctctcc
     6301 agcgtggtgc gcaatatggc cgggcccagc aacccgcacc ggcgcttgcc ggtaagcgcc
     6361 ctggccgagc gcggcattgc cgatgccgcc cagctggccg ccgcccgcgc cgatgaagcc
     6421 caaggcctgc tgcccgatgg cgcggtcatc atcgccgcca tcaccagttg caccaacacc
     6481 agcaacccgc gcaacgtgat cgctagcgcg ctgctggcac gcaacgcgcg ccgcctgggc
     6541 ctcagccgca agccctgggt caagacctcg ctcgcccccg gctccaaggc ggtagagctg
     6601 tacctgcaag aggccgggct gctggaggat ttggaagcat tgggctttgg catcgtcggc
     6661 tttgcctgca ccacctgcaa cggcatgagc ggcgcgctgg accccgccat tcaacaagaa
     6721 atcaccgagc gcggcctcta caccacggcc gtgctctcgg gcaaccgcaa tttcgacggg
     6781 cgcatccacc cccatgccaa ccaggcgttt ttggccagcc caccgctggt ggtggcctac
     6841 gcgctggccg gcacggtgcg ctttgacata gaaaacgatg tattcgccgt ggtcgatggc
     6901 aaggaggtgc gcctgcacga cctatggccc agcgatgcgg agatcgacgc cgtggtggcc
     6961 cgcgccgtcc agcccgcgca gtaccgcgcc gtgtaccagc ccatgttcag cctgcgcaaa
     7021 agcgctgccg cacccgccgc gccgcagtac gcctggcgcg cacagtccac ctacatccgc
     7081 cgcccgccgt actgggacac cgagggcgtg ggcgcgctgg cggccttccc acgcactttg
     7141 accaacctgc gcccactggc gctcttgggc gacaacatca ccaccgacca cctctcgccg
     7201 tccaacgcca tcctggccga ctcggccgcg ggcgagtact tggcaaaaat gggcctgccc
     7261 gaggaagact tcaactccta cgccacacac cggggcgacc acctcacggc cctgcgcgcc
     7321 acctttgcca acccgcaact ggtcaatgaa atggccgtgg tgggtggcca ggtgcaaaag
     7381 gggtcgctgg cgcgcatcga gcccgagggc caggtggtgc gcatgtggga ggcgatagaa
     7441 acctatctgc agcgccgcca gccgctgatc atcattgccg gggccgacta cggccagggt
     7501 tcgagccgcg actgggccgc caagggcgtg cgcctggccg gggtggaggt ggtggcggcc
     7561 gaaggctttg agcgcatcca ccgcaccaac ctgatcggca tgggcgtgct gccgctgcag
     7621 tttgcgccgg gcgtgaaccg cagcaccctg gggctggacg gcagcgagac ctacgaggtg
     7681 caaggccgcc tgaccccggg cgcgacgctg accctgctgg tgcagcgcca caatggccag
     7741 cggctgcaag tgcccatggc ctgccgcctc gataccgccg aggaggtgtc ggtgtacgaa
     7801 gccggcggtg tgttgcaacg ctttgcccag gattttttag ccaaacatgc ctctgacgcg
     7861 cttgtataaa gcgcaaacag ctatcaaaaa atgagtaaat ccgcacaact ccgcatcccc
     7921 gccacctata tgcgcggcgg caccagcaag ggcgtgttct ttcacctgcc ggatctgccc
     7981 gaatccgccc ggcaacccgg cgctgcgcgc gatgcgctgc tgctgcgcgt gctgggcagc
     8041 cccgacccct atggcaagca gatcgacggc ctgggcaatg cctcgtccag caccagcaag
     8101 gcggtcatcc tcagccgcag ccagcgcccg gggcatgacg tggactatct gtttggccag
     8161 gtggccatag accggccctt ggtcgactgg agcggcaact gcggcaacct gtcggccgcc
     8221 gtcgggccct gcgccatcca catgggcttg atagccgcgg cgcgcattcc cgagaacggc
     8281 ttgtgcacgc tgcgcatctg gcaggccaac attggcaaga ccatcgtcgc gcatgtgccc
     8341 atcacccatg gccaggtgca ggagacgggc gacttcgagc tggacggcgt ggccttcccc
     8401 gccgccgagg tggcgctgga gttcatggcc cccgccgccg aggatggggg catgttcccg
     8461 accggtcagg tgctggacat gctggatgtg cccggcgtgg gcagctttca ggccacgctg
     8521 atcaacgccg gcatccccac cgtcttcctc aacgcacaag acctgggcta caccggcacc
     8581 gagctgcagc gcgacatcaa ccaagacccg caagcgctgg cgcgcctgga ggccatccgt
     8641 gcctgggcgg cgttgcagat gggcctgatc gccgacctgg ccgaggccgc gcagcgccag
     8701 cacacgccaa aaattgcctt cgttgccccg ccggccggct acgccacgtc cagcggcaga
     8761 cgcgtcgagg ccggcgctgt cgatctgctg gtgcgcgccc tgtccatggg tcaactgcac
     8821 cacgccatga tgggcacggc ggccgtcgcc ataggcacgg ccgccgccgt gccgggcacg
     8881 ctggtcaacc tggccgctgg cggcggcacg cgcaacaccg tgtgcttcgg ccacccctcg
     8941 ggcagcctgc gcgtgggcgc cgaggtggcg cgggtggacg gccaatggca ggtgacgaag
     9001 gccgccatga gccgcagcgc gcgcattctg atggagggca tggtgcgcat tcccgccggc
     9061 agcttttaag caaaaccgcc tgactatgta catcccgcag ccgcccctca cccctcccgg
     9121 gggcgacagc gagacgctgt cgctcttcaa gctgtccagt tccgcaccgg cggaactgga
     9181 gccgcagcct tcagccccaa aggggcgagg gcgaccggtt gcgccggctt tactccctct
     9241 ccctctggga gagggtgggg gtgagggctc gcggaccagt ctgaggcatg tgcctagt
//
LOCUS       DAOMTQ010000119         9288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1507_length_9288_cov_22.992960, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000119 DAOMTQ010000000
VERSION     DAOMTQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1507_length_9288_cov_22.992960"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..953
                     /gene="ylqF"
                     /locus_tag="PK518_11365"
     CDS             3..953
                     /gene="ylqF"
                     /locus_tag="PK518_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HRM49557.1"
                     /translation="MQWFPGHMHLTRKAIGERIKDIDVVIELLDARLPGSSSNPLLAE
                     LTAHKPTLKVLNKQDLADPGRTGAWLAWYNARPDTSAIALDASDAAPARRLIDACRQL
                     APNRRGLSKPMRVLICGIPNVGKSTLINTLSRKTQAKTGDEAGITKQEQRIVLDDDFY
                     LWDTPGMLWPRIIVPESGLRLAASGAMGRNAYDEEEVVLELLAYLKQHYAPLIDARYK
                     LGLDAEAIAARHDEELLELIAKKRGAVMSGGRLNMQKAAEIVLTDLRGAALGRLTLET
                     VPEFEAWLAAGQLQDKARAERKAEQRKRDERRARNAQRRE"
     gene            complement(1488..3323)
                     /locus_tag="PK518_11370"
     CDS             complement(1488..3323)
                     /locus_tag="PK518_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49558.1"
                     /translation="MPDLTASTAHTESDMNQADLPLDGTLCPGEAMHGQHLHQLLRAA
                     LPDSSHHKAAGWFGQTDFLFTRRHVDEDQDAYHDGLFITLWKRWDFWLALPDDSTGPG
                     GRLAQAWARLRQDHHPALADGLLRQYRAYDDAGRPGPWQPLDADHASPEALQACAQAW
                     RGVIDYWRLAEGQRRAGRPLRERLHAALTTARVQALTLLPVFTGRYNDWWNPERNGWW
                     QGDVWIGARQPGHQGSRHWGRALKLSWRNGSEQPGDEEDDAHACYQIDLVEDDTPAQG
                     TAQPPGLRLSYGQRQSDARRPLTNDAVQHMARLLQLFTDLEQQLYAANAREQRALRCA
                     PLAPAGAAPALPRRPPFDPDETDADVLGPAIMALSHDWQSSGRSHATLVRERWTAQAE
                     GLRTPDAQHAGAPAPPALVDPRMGQATDVLQLAHAVHALADPDLSARFHRRFAFAPGA
                     FEHHAARSGRAVGPLLWQADGSLLAHVDGASGTADQTGAWWQLSADRRSMTALPPAQA
                     PATVTSADASSTAHGVTVRGDGAGDLHGLAEDGRALWRHHVGGAIRSIAPAPDGHTLA
                     VGTAGGYLVLLHKGSGTDPYLVSNSRYAEARRFIFWGDAPAPLAW"
     gene            complement(3386..4156)
                     /locus_tag="PK518_11375"
     CDS             complement(3386..4156)
                     /locus_tag="PK518_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM49559.1"
                     /translation="METQPGASAHPPLSILWQDEHLVALYKPAGWLVHRTGLDAGETR
                     FVVQTLRDQIGQHVYPVHRLDKGTCGVLVMGLHPAATRALAGAFAAHAVRKQYLALVR
                     GWAPESAEVNHPLRPDDAPPDATAQDAHTTITRLARLDLPEPSDARHAGTRASLVLAQ
                     PTTGRRHQIRRHLKHLAHPIIGDATHGKGPLNRWWAERLGLQRLWLHAWRLQLPHPVD
                     GRWITLDSGLRWPQATPGDGAAPWRDWQRVLALPWQND"
     gene            4217..5140
                     /locus_tag="PK518_11380"
     CDS             4217..5140
                     /locus_tag="PK518_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRM49560.1"
                     /translation="MMSTRSTLLPLPLLAAPSLNDPQPLVLYHDRCADGFAAALAAWR
                     FYGGQVQCLGLSHGQIRNVEELPPLAGRAVYVLDFSFGPELLAEIDARAAKLVLLDHH
                     KSAAEQLAGFACRCGALHFDMTKSGARLAWELFHPEAPLPDLVRHVEDRDLWAWQYPE
                     TAGYVAALDMEPFDFARWQQIADFAPAETAAFVARGQAMDEKFRHLAQDVAGGAQPLL
                     FNGQAGLMVNAPGAFHSLVGELLSKQCGTFALMWAVAKDGQVKVGLRSQRGYDCSPLA
                     VSMGGGGHAQACGFRLPADRLPELLGGNFKT"
     gene            5147..6601
                     /locus_tag="PK518_11385"
     CDS             5147..6601
                     /locus_tag="PK518_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRM49561.1"
                     /translation="MATPAPISIDDITALDASALSQAIHARSVSCRAVMQAYLERIQR
                     LNPRYNALVSLRPEDDLLADADACDAELARGQSRGWMHGMPQAIKDLSNAAGLPTTLG
                     SPLMRGFVANEDGLMVARMKAAGCIVIGKSNTPEFGLGSHTFNPVFGTTLNAYDPARS
                     AGGSSGGAAVALALRLLPVADGSDFMGSLRNPAAWANIFGFRPSQGRVPMWPAQDVWI
                     SQLGTEGPMGRSVRDLQRLLATQAGWSPNAPLSIAEGAYPEMAGGLFDVKSTRIAWLA
                     DLDGYLPMEPGILDICAQGLRRLESLGCSVQPCALGMPPERVWQAWLVWRRVLVASRI
                     APFLLKEGNRAHIKPEALWEHDQAAQVSGADFMGASVARTSFYQQLLRLFEHHDVLAL
                     PSAQVWPFDAAERWPQQVAGLAMDSYHRWMEVVIYATFAGLPCISVPTGFNAQGLPMG
                     LQLIGRPHGDAALLALAAAYEGAIGDWLAVRPGA"
     gene            complement(6794..7234)
                     /locus_tag="PK518_11390"
     CDS             complement(6794..7234)
                     /locus_tag="PK518_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRM49562.1"
                     /translation="MPASTALLCLVVAIHDGDTLSARCGAPGAWRQERVRIAVVDAPE
                     SRQAFGQRARQNLARLCFRQQARLYRLGHDSYGRTLAHVRCQGQDVATAQLRAGLAWV
                     YAPQAQQHPRLVALQRQARANGQGLWAQKRPLAPWDYRRQYLRP"
     gene            7785..8666
                     /gene="rarD"
                     /locus_tag="PK518_11395"
     CDS             7785..8666
                     /gene="rarD"
                     /locus_tag="PK518_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRM49563.1"
                     /translation="MFKGLVASVVASVLFGAMYYLAPQLAPLDGEQIFGWRVLATLPF
                     TTVLLLALGQWRRVRLLLRRARRQPSLALGLLASAALLGVQLWLFLWAPLHGRALPVS
                     LGYFLLPLVMVLAGRVLYRESLTGLQWLATLLAGAGVAHEVLRAGGMSWETWLVALGY
                     TAYFVLRRRLATDHLGGHWLDMLLLVPAALWFVARAPSSLGLVAGHQHLWAMVPVLGL
                     VSAVALALYMAASRLLPLGLFGLLSYVEPVLLALVALALGESIGREQWPSYGPIFAAV
                     GVLVLDGALRLGQSQKV"
     gene            complement(8701..9288)
                     /locus_tag="PK518_11400"
     CDS             complement(8701..9288)
                     /locus_tag="PK518_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM49564.1"
                     /translation="MRRPAPAAPRLLRFNKPYGVLSQFTPEGRWRGLKDFITEPGVYV
                     AGRLDADSEGLLLLTNDGPLQARISDPRHKMEKTYWVQVEGIPDDAALDALRHGVALN
                     DGPTLPARVRRLDPPPTLWPRDPPIRVRQNIPDCWLELIIREGRNRQVRRMTAAVGHP
                     TLRLVRAAIGPYRLDGLAPGCWADADVPNYQKKSC"
BASE COUNT         1275 a         3128 c         3393 g         1492 t
ORIGIN      
        1 ccatccagtg gttccccggt cacatgcacc tgacgcgcaa ggcgataggc gagcgcatca
       61 aggacatcga cgtggtgatc gagctgctgg acgcgcgcct gcccggctcc agcagcaacc
      121 cgctgctggc cgagctgacc gcgcacaagc ccacgctcaa ggtgctgaac aagcaggacc
      181 tggccgaccc cgggcgtacc ggcgcctggc tggcctggta caacgcgcgg cccgacacca
      241 gcgccatcgc gctcgatgcc tccgacgcgg cgcccgcgcg ccggctgatt gatgcctgcc
      301 gccagctcgc gcccaaccgg cgcggtctct caaagcccat gcgggtgctg atatgcggca
      361 ttcccaatgt cggcaagtcc acgctgatca acaccctgag ccgcaagacc caggccaaga
      421 ccggcgacga ggccggtatc accaagcagg agcagcgcat cgtgctcgac gatgacttct
      481 acctgtggga cacgcccggc atgctctggc cgcgcatcat cgtgccagag agcggcttgc
      541 gcctggccgc cagcggcgcc atggggcgca acgcctatga cgaggaagag gtggtgctgg
      601 agctgctggc ctacctcaag cagcactacg cgccgctgat cgatgcgcgc tacaagctgg
      661 gcctggatgc agaggcgatc gctgcccgcc acgacgaaga gctgttggag ctgatcgcga
      721 aaaagcgcgg cgccgtgatg agcggcggcc gcctgaacat gcaaaaggcc gccgagatcg
      781 tgctcaccga cctgcgcggc gcagcgctgg gccgcctgac gctggagacg gtgcccgagt
      841 tcgaggcctg gctggccgcc ggccagctgc aggacaaggc gcgcgccgag cgcaaggccg
      901 agcagcgcaa gcgcgacgag cgccgggcgc gcaatgcgca gcgccgtgaa tagttgcagg
      961 ccggaaccgg tttgcagcgc cgctcggtga tcccggagct ggccgttttt gtgttgcaac
     1021 agcttgatta ggcatatgcc tctgaccgct gtcttgactc tctcccactc tgggggaggg
     1081 cggggtgggg ccagcgacgc tcgcgtcctg atgcctgcgc tcctggaccc tatcccaacc
     1141 ttcccccaga gggcatgggt gtctacacac ctttttcgta gcgcagaacg cttgtttgac
     1201 ggggcttcaa ctccctcgcc cccttggggg agagggtggg ggtgaggggg tgatggggcg
     1261 cagcgcgggc attggccccc tcaccccccc tctcccgcca gcgggagagg gagaaacagc
     1321 cccagggccg cacacatgtt gcgtggcacg ctatacaaaa gatgtgtaga cacccaggcc
     1381 cagaggggga aggagtttga gtcatgccga agatttggta aaaaaggctg gtatcgccct
     1441 tgggacaagc gctgatagct atcaaaaaag aagtgtaaac cttgccgcta ccaggccagc
     1501 ggcgccggcg catcacccca gaagatgaag cgcctggcct cggcgtagcg cgagttgctg
     1561 accagatagg gatcggtgcc gctgcccttg tgcagcagca ccaggtagcc gccggccgtg
     1621 cccacggcca gggtgtggcc gtcgggtgcc ggggcgatgc tgcggatcgc gccgccgaca
     1681 tggtggcgcc acagggcgcg gccgtcctcg gccaggccgt gcaggtcgcc ggccccgtcg
     1741 ccgcgcaccg tcacgccgtg ggccgtgctg ctggcgtcgg cgcttgtcac ggtggcgggc
     1801 gcctgcgccg ggggcagggc ggtcatgctc cggcgatcgg cactgagctg ccaccaggcg
     1861 ccggtctgat cggccgtgcc gctggcgcca tcgacatggg ccagcagact gccatcggct
     1921 tgccacagca atggccccac ggcccggccg ctgcgcgcgg catggtgctc gaaggcgccg
     1981 ggcgcaaagg caaagcggcg gtgaaagcgt gccgacaagt ccgggtccgc cagcgcatgc
     2041 acggcgtgtg ccagctgtag cacatccgtg gcctggccca tgcgcggatc gaccagcgcc
     2101 ggcggcgcgg gggcgccggc atgctgcgcg tcgggggtcc gcaggccctc ggcctgtgcc
     2161 gtccagcgct cgcgcaccag ggtggcgtgg ctgcggccgc tgctctgcca gtcatgcgac
     2221 agggccatga tggcggggcc gaggacatcg gcgtcggttt cgtccggatc aaacgggggt
     2281 ctgcgcggca gggctggcgc ggcgccggcc ggggccaggg gggcgcagcg cagcgcccgc
     2341 tgttcgcgcg cattggccgc gtacagctgc tgctccagat cggtaaacag ttgcagcagc
     2401 cgcgccatat gctgcacggc gtcgttggtc agggggcggc gggcgtcgct ctggcgctgg
     2461 ccatagctca gacgcaggcc gggcggctgg gccgtgccct gggccggggt gtcgtcctcg
     2521 accaggtcga tctggtagca ggcgtgggcg tcgtcctcct cgtcgccggg ctgctcgctg
     2581 ccgttgcgcc agctcagttt cagggcccgg ccccagtgcc ggctgccctg gtggccgggc
     2641 tggcgtgcgc ctatccacac atcgccctgc caccagccgt tgcgctcggg gttccaccag
     2701 tcgttgtagc ggccggtgaa caccggcagc agcgtcaacg cctgcacgcg cgcggtggtc
     2761 agggcggcgt gcagccgctc gcgcagcggc cggccggcgc ggcgctggcc ctcggccagg
     2821 cgccagtagt cgatcacgcc gcgccaggcc tgggcgcagg cctgcagcgc ctcggggctg
     2881 gcatggtctg catccagcgg ctgccatggg ccggggcggc cggcatcgtc ataggcgcgg
     2941 tactggcgca gcaggccgtc tgcaagcgcg ggatgatggt cctggcgcag cctggcccag
     3001 gcctgggcca ggcgcccgcc tgggcccgta ctgtcgtcgg gcagggccag ccagaagtcc
     3061 cagcgcttcc agagggtgat gaacaggccg tcgtggtagg cgtcttggtc ctcgtccaca
     3121 tggcggcgcg tgaacagaaa gtcggtctgc ccgaaccagc ccgcggcctt gtggtggctg
     3181 ctgtcgggca gcgcggcgcg cagcagctgg tgcaggtgct ggccgtgcat ggcctcgccg
     3241 gggcagagcg tgccgtccag cggcaggtcg gcctggttca tgtcggactc ggtgtgcgcg
     3301 gtgctggcgg tgaggtcggg catggcgggc tgggggcagt ggcggggccc atggtagcgc
     3361 ggcggcgcgc ggctcaggcg ttctttcagt cgttctgcca gggcagggcc aacacgcgtt
     3421 gccagtcccg ccagggcgcg gcgccgtcgc ccggtgtcgc ctggggccag cgcaggccgc
     3481 tgtccagggt gatccatcgc ccgtccaccg ggtgcggcag ttgcaggcgc caggcgtgca
     3541 gccacaggcg ctgcaggccc aggcgctcgg cccaccagcg gttcagcggg cccttgccgt
     3601 gggtggcgtc gccgatgatg gggtgggcca ggtgcttcag gtggcggcgg atctggtggc
     3661 gccggccggt ggtcggctgg gccagcacca ggctggcgcg ggtgccggca tggcgcgcgt
     3721 cgctgggctc gggcaggtcc agccgggcca ggcgggtgat ggtcgtgtgg gcgtcctggg
     3781 ccgtcgcatc gggtggggcg tcgtcggggc gcagtgggtg gttgacctct gcgctctcgg
     3841 gcgcccagcc gcgcaccagg gccaggtatt gcttgcgcac ggcatgcgcg gcaaaggccc
     3901 cggccagggc gcgcgtggcg gccgggtgca ggcccatgac gagcacgccg caggtgccct
     3961 tgtccaggcg gtgcacgggg tagacatgct ggccgatctg gtcgcgcagg gtctgcacca
     4021 cgaagcgcgt ctcgccggcg tccagccccg tgcggtgcac cagccagccg gcgggcttgt
     4081 acagcgccac caggtgctcg tcctgccaca ggatggacag aggtgggtgg gcgctggcgc
     4141 ctggctgggt ttccatgaat ggtgagacga agtgggcagg ccgggcatgg tatcggctgg
     4201 cgcacaatgc gccgccatga tgtccacacg ctccacgctg ctgccgctgc ccctgctggc
     4261 ggccccctcg ctcaacgatc cccagcccct ggtgctgtac cacgaccgct gcgccgacgg
     4321 ttttgccgcc gcgctggccg cctggcgctt ctatggcggg caggtgcaat gcctgggcct
     4381 gtcgcacggg cagatccgca atgttgaaga gctgccgccg ctggccggcc gggccgtcta
     4441 tgtgctggat ttttccttcg ggcccgagct gctggccgag atcgacgcac gcgccgccaa
     4501 gctggtgctg ctggaccacc acaagagcgc ggccgagcag ctggccggct ttgcctgccg
     4561 ctgtggcgcg ctgcatttcg acatgaccaa gtcgggcgca cgcctggcct gggagctctt
     4621 tcaccccgaa gcgccgctgc ccgacctggt gcgccatgtg gaagaccgcg acctgtgggc
     4681 ctggcagtac cccgagacgg cgggctatgt ggcggcgctg gacatggagc ccttcgactt
     4741 tgcacgctgg cagcagattg ccgactttgc gccggccgag acggcggcct tcgtcgcgcg
     4801 cggccaggcc atggacgaaa aattccgcca cctggcgcag gacgtggccg gcggcgccca
     4861 gccgctgctg ttcaacggcc aggccgggct gatggtgaac gcgcccgggg ccttccacag
     4921 cctggtgggc gagctgctgt ccaagcaatg cggcaccttc gcgctgatgt gggccgtggc
     4981 caaggacggc caggtcaagg tcggcctgcg ctcgcagcgc ggctacgact gctcgccgct
     5041 ggctgtcagc atgggcggcg gcggccacgc ccaggcctgc ggctttcgct tgcccgcaga
     5101 tcgcctgccc gagctgctgg gcggcaactt caagacatag gcaaatatgg ccacgcccgc
     5161 gcccatctcc atcgacgaca tcaccgcgct cgacgccagc gcgctgtcgc aagccattca
     5221 tgcccgcagc gtctcctgcc gcgccgtcat gcaggcctac ctggagcgca tccagcgcct
     5281 gaacccgcgc tacaacgcgc tggtttcgct gcggcccgag gacgacctac tggccgatgc
     5341 cgatgcctgc gacgccgagc tggcgcgcgg ccagtcgcgc ggctggatgc acggcatgcc
     5401 gcaggcgatc aaggacctgt ccaacgctgc cggcctgccc accacgctgg gttcgccgct
     5461 gatgcggggc ttcgtcgcca acgaggacgg gctgatggtc gcacgcatga aggcggccgg
     5521 ctgcatcgtc atcggcaaga gcaacacgcc ggagttcggc ctgggttcgc acaccttcaa
     5581 cccggtgttc ggcaccacgc tgaacgccta tgaccccgcc aggtcggccg gcggctccag
     5641 cggtggcgcg gccgtggcgc tggcgctgcg gctgctgccg gtggccgacg gttcggactt
     5701 catgggcagc ctgcgcaacc cggcggcctg ggcgaacatc tttggcttca ggcccagcca
     5761 gggccgcgtg cccatgtggc cggcgcagga cgtgtggatc agccagctgg gcaccgaagg
     5821 ccccatgggg cgtagcgtgc gtgacctgca gcgcctgctg gcgacacagg cgggctggtc
     5881 gccaaatgct ccactatcaa tagctgaagg cgcttatcct gaaatggctg gcggcctgtt
     5941 tgatgtcaaa tccacgcgca tcgcctggct ggccgatctg gacggctacc tgcccatgga
     6001 gcccggcatt ctggacatat gcgcccaggg gctgcggcgc ctggaaagcc tgggctgcag
     6061 cgtgcagccc tgcgccctgg gcatgccgcc cgagcgcgtg tggcaggcct ggctggtgtg
     6121 gcggcgcgtg ctggtggcgt cgcgcattgc ccccttcctg ctgaaggagg gcaaccgcgc
     6181 ccatatcaag cccgaggcgc tgtgggagca tgaccaggcg gcgcaggtca gcggcgccga
     6241 tttcatgggc gccagcgtag cgcgcaccag cttctaccag cagctgctgc gcctgttcga
     6301 gcaccatgac gtgctggccc tgcccagcgc ccaggtgtgg cccttcgacg ctgccgagcg
     6361 ctggccgcag caggtcgccg gcttggccat ggacagctac caccgctgga tggaggtggt
     6421 catctacgcc acctttgccg gcctgccctg catcagcgtg cccaccggct tcaatgccca
     6481 gggcctgccc atggggctgc agctgatcgg ccggccgcac ggtgatgcgg cgctgctggc
     6541 cttggcagcg gcctacgaag gggcgatagg ggactggctg gcggtgcggc cgggggcctg
     6601 aacgcacgcc agcctccctg ccggctatgg ctagtacagc aacccggaaa tatcgaaaca
     6661 tgaaagcgcg ccttcgcccc catggcggcg ttgcaaatcc tcgcgatagc tacggctatc
     6721 gctgcggttt gcgccttgcc ctgtgcgcga atccatcgct ttcattgtgt tcctctactt
     6781 ccaggttgct gtactagggc cgcaggtact ggcgccgata gtcccagggc gccagcgggc
     6841 gcttttgcgc ccacagcccc tggccattgg cgcgtgcctg gcgctgcagg gccaccagcc
     6901 tggggtgctg ttgtgcctgc ggcgcataca cccaggccag gccggcgcgc agctgggccg
     6961 tggccacgtc ctggccttgg cagcgcacat gggccagggt gcggccgtag ctgtcgtggc
     7021 ccaggcggta cagccgcgcc tgctggcgaa agcacagccg ggccaggttc tggcgcgcgc
     7081 gctggccaaa ggcctggcgg ctctcggggg catccacgac ggcgatgcgc acgcgctcct
     7141 ggcgccaggc gccgggcgcg ccgcagcgcg cgctgagggt gtcgccgtcg tggatggcga
     7201 caaccaggca gagcagggcg gtagaggcgg gcatgggggc ggcggcagga caaaaggggg
     7261 cgcactatac ccgctgcggg ccgcggccat ggtcgcggca ggcgctgcct aaaaaatcag
     7321 cgatgcgctg cagcccaata ccggcgcggc ttggggctgg ttgtaccggt ggttttccac
     7381 agggccgccc acaggccgtg ggcataaccc tagaaacggc agttgaccgc tttcaaactt
     7441 cacgaaagcc gcgtgaatat tgaggtttgc tgcttcgtcg gcactcacct gtggggtgac
     7501 aacgctacgc tacttgtcag taccgcgctc gccgcgccat gcggcgttgc tcctccttgc
     7561 gggcagtagc ccgctgcggc gtcccccttg cagggcgcgg tcccgccaga ggcggaaccc
     7621 gctcatgccg tccaagggtg cacaggcacc cttggagccg cggcattcgc ccttgcctgg
     7681 cacgccgatc acggcactgc caagcatgtc caactgccgt ttctagggta actgcgcccg
     7741 ccgatgaccg acaatcaccg gttttcctcg cagcccccat acccgtgttc aaaggtcttg
     7801 tagcgtccgt cgtggcgtcg gttttgtttg gcgccatgta ctacctggcg ccccagctgg
     7861 cgccgctcga tggcgagcag atcttcggct ggcgcgtgct ggccacgctg cccttcacca
     7921 ccgtgctgct gctggcgctg ggccagtggc ggcgcgtgcg cctgctgctg cggcgcgcac
     7981 ggcgccagcc gtcgttggca ctggggctgc tggccagcgc ggcgctgctg ggcgtgcagc
     8041 tgtggctgtt cttgtgggcg ccgctgcacg ggcgcgcgct gccggtgtcg ctgggctact
     8101 ttctgctgcc gctggtgatg gtgctggccg ggcgggtgct gtaccgcgaa agcttgaccg
     8161 gcctgcagtg gctggccacg ctgctcgcgg gcgccggcgt ggcgcatgag gtgctgcgcg
     8221 ccggcggcat gtcgtgggag acctggctgg tcgccctggg ctacacggcg tacttcgtgc
     8281 tgcgccgccg tctggccacc gaccacctgg gcgggcactg gctggacatg ctgctgctgg
     8341 tgcccgcggc cctgtggttc gtggcgcgcg cgccctcgtc gctgggcctg gtggccggcc
     8401 accagcacct gtgggccatg gtgccggtgt tgggcctggt gagcgccgtg gcgctggcgc
     8461 tgtacatggc cgccagccgg ctgctgccgc tgggcttgtt tggcctcttg agctatgtgg
     8521 agccggtgct gctggccctg gtggcgctgg ccctgggcga gagcattggc cgcgagcagt
     8581 ggccgagcta cgggcccatt ttcgcggccg tgggcgtgct ggtgctcgat ggcgcactgc
     8641 gcctgggcca gtcgcagaaa gtttgatgga aatacggccg tagcgcccgt gggataagcg
     8701 ttaacagctc tttttttgat agtttggtac gtcggcatcg gcccagcaac cgggtgccag
     8761 gccgtccagc cggtacggcc caatggccgc gcgcaccagg cgcagcgtgg ggtgacccac
     8821 ggcggccgtc atgcggcgca cctggcggtt gcggccttcg cggatgatga gctccagcca
     8881 gcagtccggg atgttctggc gcacgcgtat cggcgggtcg cgcggccaca gcgtaggcgg
     8941 cgggtctagc cggcgcacgc gcgcgggcag ggtggggccg tcgttcagcg ccacgccatg
     9001 tcgcagcgca tccagtgcgg cgtcgtcggg aatgccctcg acctgcaccc agtaggtctt
     9061 ctccatcttg tggcgcgggt cgctgatgcg cgcctgcagg gggccgtcgt tggtcagcag
     9121 cagcaggccc tcgctgtcgg cgtccaggcg accggcgaca tagacgccgg gctcggtgat
     9181 gaagtccttg aggccacgcc agcgtccctc gggcgtgaac tggctcagca cgccgtaggg
     9241 cttgttgaag cgcagcaggc gcggggcggc gggcgccggc cggcgcag
//
LOCUS       DAOMTQ010000120         9250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1523_length_9250_cov_21.801414, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000120 DAOMTQ010000000
VERSION     DAOMTQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_9250_cov_21.801414"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="PK518_11405"
     CDS             <1..1838
                     /locus_tag="PK518_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49565.1"
                     /translation="RQTLDRAQKFLSLVALLAALLSAVAVALAARGFANEHLDAAALL
                     RVLGQSQRSIAGAYAFEFALVGLFASALGVLLGFAVHHVFVWLLAGLVQSGLPAASLW
                     PVVLGVGVGLTLMCAFGLPPVLQLAQVPPLRVLRRDLGAVRPASLAVLGLGVAGFAAL
                     LLVVSRDWKLGAIAVGGFAAAVLLFALLAWMAVWLLKKSVHESTAPRALVLATRQIAA
                     RPAYAVVQVSSLAVGLLALVLLVLLRTDLIASWQQATPANAPNRFVINIMPDQAEDFR
                     ARLAQAGVAQYDWYPMFRGRLVAINGRAVSLDDYPDERAKGLVDREFNLSNASQPPTH
                     NSISAGRWTPEEAGAVSVEEGIAKTLGLKLGDQLRFDIGGVPSEVRITSLRHVDWSSM
                     RANFFVMYPLAQMQDVPMTYLAAYRAPEVWPPRSSAAQASLPPEGSAASPGRPGVGSA
                     GFDNALVRAFPNVTNVDMSATLAQVQGVLGQVIRAVEFLFAFTLAAGLVVLFAAVTAT
                     REERAREFAIMRAVGARASLLRQVQRAELLGVGLLAGLLASCVAVAVGWLLARHVFDF
                     SWTVRPWVPLAGSLVGALLALTAGWWGLREVLQRPVVQTLRRAQD"
     gene            1848..2069
                     /locus_tag="PK518_11410"
     CDS             1848..2069
                     /locus_tag="PK518_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49566.1"
                     /translation="MQTNEDWTPDWSQAPEGWDWLAQDEDGRWFWYRTEPTPGFGGGV
                     WRANSRNQQYAGQGRPNALWMDSLRRRYY"
     gene            2140..3660
                     /locus_tag="PK518_11415"
     CDS             2140..3660
                     /locus_tag="PK518_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM49567.1"
                     /translation="MAQALGAALEAAGDAPVQHLQTHLSHLLLTPTYAYKLKKPLRLP
                     FADFSTLAARRHFCNEELRLNQRLAPQLYLDVLPVLGSVRQPRIGSAHEAGAAIDWVL
                     RMRRFAPGSELDALVRAGQLQGFELDAFAARLAHFHAQAPVAGQDSDWGRPAQVGQTI
                     ADVLDQLAALLSGAEAARLAALRAWFGARQPALAGHWAARRAAGQVRECHGDLHLGNV
                     VRIDGEITAFDCLEFSPALRWIDTLADAGFLTMDLHAHGRPDLAWRFLDAYLAASGDY
                     AGLAVLRPYEVYRALVRTLVARLRASQGGAAQGPDYLACAERLAAPLGARLLITYGLS
                     GSGKSTAAAQLLQQGAVRLRSDVERKRLHGLAPLDDSAALGLDIYTSEASRRTFAQLA
                     AQARALLAAGYPVIVDAAFLRRRERDAFHALARELGLPFAILHCHADPDRLRERVRQR
                     RAAGGDPSEADLAVLARQQQQAEPLAPEEQERTLDLATDQAWDAPALYRRWLSVQA"
     gene            3704..4096
                     /locus_tag="PK518_11420"
     CDS             3704..4096
                     /locus_tag="PK518_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRM49568.1"
                     /translation="MNTIALTDSQREQLRSALELRKEQLLQELDAVQRDTLAVASAAS
                     RGQADPAGSPRDQANSMAADMVRDAEAARDHAELTAVRAALERLADGSYGECTDCGQS
                     VGVPRLLAQPSAARCIACQSKAETQVRR"
     gene            complement(4255..4917)
                     /locus_tag="PK518_11425"
     CDS             complement(4255..4917)
                     /locus_tag="PK518_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRM49569.1"
                     /translation="MTAPLKAQSFADLKPLRRTLQERAAQQAERERLRREAEQRARAE
                     RHLFQDAVGAVQPLKGQHERHWPAPVPPEPLPVQRQLDEARVLHESLSDEFDVSTLLD
                     VDDQLSFRRTGMGPDVTRWLRAGQWSIQRQLDLHGLRVDEAREALGQFIRLAHRTGLR
                     CVRVVHGKGLGSPGKTPVLKGRVQGWLVQKKEVLAFVQARPADGGAGALVVLLAPSPR
                     RI"
     gene            complement(4914..6083)
                     /locus_tag="PK518_11430"
     CDS             complement(4914..6083)
                     /locus_tag="PK518_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRM49570.1"
                     /translation="MSLARWACIALLALAGCALPLAPPAPAPLLAPHLRLIGVAELAH
                     GSEYAGARIGGLSGIDYDAHSGEYLLISDASARIYRARLHYDAQRLDMPQFTATQDLL
                     HTSGQPFAPWWRRQAGMDRPDAEGLRWLPGKTSFLWSSEGDFSRGFGPQLRESRLDGR
                     PVRDIALPASFSPGTQPRRGPRGNGTLEGLALTPDGRTAWLAMELPWHQDGPQATPDS
                     GGAPVRITAMDIASGQPLRQIAYQGDAVPQRRRLPGPQINGVSEILAHGPHHLLVLER
                     SYSAGAGFGARLYRIDTRAGSDTLTLDALTPANHRPVPKTLVADLAALGLTPDNIEGM
                     TWGPPVQGRCVLVFVSDDNFNPAQVTQFIAAQYLGPDGDGGQCGTTGASVLSTYP"
     gene            complement(6080..6793)
                     /gene="radC"
                     /locus_tag="PK518_11435"
     CDS             complement(6080..6793)
                     /gene="radC"
                     /locus_tag="PK518_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRM49571.1"
                     /translation="MPLKDLPFDAQPREKLLARGPAALSDAELLAILLRTGMAGKGVL
                     QLAQELLDEPRRDAGGRITGGGFGGMAGLLHAGAADLARIKGLGPAKRAELVAVLELA
                     RRALAQQLREREVFGTPGAVKHYLQLHLAARAHEVFAVLFLDSQNRLLAMEELFRGTL
                     TQTSVYPREVVLRALQHQAAAVVLAHNHPSGSVQPSRADEALTQTLKAALALVDVRVL
                     DHVIVAPGVALSMAEQGLV"
     gene            6858..7334
                     /locus_tag="PK518_11440"
     CDS             6858..7334
                     /locus_tag="PK518_11440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRM49572.1"
                     /translation="MTADALASDTSQPIVQMGSFLTLHYRLAGPAGDVVNTFEGKPAT
                     LSVGTGELSPALEQRLIGLAEGARARFELAAGEAFGERNPDMRQWVGRKLLNDLGDPD
                     EQYHVGDVVEFPTPDGAGKYAGAVLEVRGDGAVRFDFNHPLAGLPVTFEVQLIGVL"
     gene            7335..8330
                     /gene="ispH"
                     /locus_tag="PK518_11445"
                     /gene_synonym="lytB"
     CDS             7335..8330
                     /gene="ispH"
                     /locus_tag="PK518_11445"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRM49573.1"
                     /translation="MNKPQEIVLAEPRGFCAGVDRAIEIVERALQKFGRPIYVRHEIV
                     HNTYVVNDLKSKGAIFIEDLAEVPPGATLIFSAHGVSRAVQDEARARGFSIFDATCPL
                     VTKVHVEVAKLAREGYEFIMIGHKGHPEVEGTMGQLDSGIHLVEDVADVARVQPAQTE
                     KLAVVTQTTLSVDDAAEITAAVRARFPSIREPKQQDICYATQNRQDAVKLLSPQVDLV
                     IVVGSPTSSNSNRLRELAARLGTAAHMVDSADELRAEWFDGIARVGLTAGASAPEVLV
                     QAVITRIKELGAVSVRKMSGIEESVKFPLPKGLKLDAATGLELAERSPETGASSG"
     gene            8560..>9250
                     /locus_tag="PK518_11450"
     CDS             8560..>9250
                     /locus_tag="PK518_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM49574.1"
                     /translation="MEIAILFALILLNGVFAMSELALVSARKTRLQKLIDEGDSGAIA
                     AVKLGEDPTRFLSTIQIGITSIGVLNGIVGESALAKPFGEWLIGLGLQAQYASYIATG
                     LVVVLITYFSIVVGELVPKRLGQSHPEALARIVARPINWLALATKPFVRLLSISTETL
                     LRALGVKQGQGNAVTEDEIHAVLAEGTSAGVIESHEHQMVRNVFRLDDRQIGSLMVPR
                     ADVVCLDVLESF"
BASE COUNT         1269 a         3202 c         3317 g         1462 t
ORIGIN      
        1 tgcgccagac gctggatcgg gcgcaaaaat tcttgagcct ggtggcgctg ctggcggcgc
       61 tgctgtcggc cgtggccgtg gccctggccg cgcgcggctt tgccaacgag catctggatg
      121 ccgcggcgct gttgcgcgtg ctcggccaaa gccagcgcag cattgccggc gcctatgcgt
      181 tcgaattcgc gctggtgggc ctgtttgcca gcgccctggg cgtgctgctg ggctttgccg
      241 tgcaccatgt gttcgtctgg ttgctggccg ggctggtgca gtctggcctg ccggcggcca
      301 gcctctggcc ggtggtgttg ggtgtgggcg tgggcctgac gctgatgtgc gcctttggcc
      361 tgccgcccgt gctgcagctg gcccaggtgc cgccgctgcg cgtgctgcgc cgtgacttgg
      421 gggccgtgcg tcccgcgtcc ctcgccgtgc tgggcctggg cgtggccggc tttgccgcgc
      481 tgctgctggt ggtaagccgc gactggaagc tgggcgcgat tgccgtgggc ggctttgccg
      541 cggccgtgct gctgtttgcg ctgctggcct ggatggccgt gtggctgctg aagaaaagcg
      601 tgcatgaatc caccgcgccg cgcgccctgg tgctggccac gcgccagatc gccgcgcgcc
      661 cggcctatgc cgtggtgcag gtcagcagcc tggccgtggg cctgctggcc ctggtgctgc
      721 tggtgctgct gcgcaccgac ctgatcgcca gctggcagca ggccacaccg gcgaatgcgc
      781 ccaatcgctt tgtgatcaac atcatgccgg accaggccga ggactttcgc gcgcggctgg
      841 cgcaggcggg cgtggcgcaa tacgactggt accccatgtt ccgcggccgc ctggtggcca
      901 tcaacggccg tgccgtgagc ctggacgact accccgacga gcgcgccaag ggcctggtcg
      961 atcgcgagtt caacctttcc aacgccagcc agccgccgac gcacaacagc atctccgccg
     1021 gccgctggac gcccgaggag gccggcgccg tgagcgtgga ggagggcatt gccaagacac
     1081 tgggcctgaa gctgggtgac cagctgcgct ttgacatcgg cggcgtgccc agcgaggtgc
     1141 gcatcaccag cctgcgccat gtggactgga gcagcatgcg tgccaacttc ttcgtcatgt
     1201 acccgctggc gcagatgcag gacgtgccca tgacctacct ggctgcctac cgcgctcctg
     1261 aagtgtggcc cccacgctct tctgccgcgc aagcttcgct gccccccgag gggtctgctg
     1321 ccagccccgg gcggcccggc gttggcagcg ccggctttga caacgccctg gtgcgcgcct
     1381 tccccaacgt caccaacgtg gacatgagcg ccaccctggc ccaggtgcag ggcgtgctgg
     1441 ggcaggtgat acgtgccgtg gagttcttgt tcgccttcac gctggccgcc ggcctggtgg
     1501 tgctgtttgc cgccgtcacc gccacgcgcg aggagcgcgc gcgcgagttc gccatcatgc
     1561 gcgccgtggg cgcgcgcgcc agcctgttgc gccaggtgca gcgcgccgaa ctgctgggcg
     1621 ttggcctgct cgccggcttg ctggccagtt gcgtggcggt ggccgtgggc tggctgcttg
     1681 cgcgccatgt gttcgacttc agctggacgg tgcgcccctg ggtgccgctg gccggcagcc
     1741 tggtgggcgc tctgctggcg ctgaccgcgg gctggtgggg gctgcgcgag gtgctgcagc
     1801 gcccggtggt gcagacgctg cgccgggcgc aggattgagg ggcagccatg caaacgaacg
     1861 aagactggac gcccgactgg tcgcaggccc ccgagggctg ggactggctg gcgcaggacg
     1921 aggacgggcg ctggttctgg taccgcaccg agcccacgcc cggctttggc ggtggcgtct
     1981 ggcgcgccaa ctcgcgcaac cagcaatatg ccggccaggg gcggccgaat gcgctgtgga
     2041 tggactcgct gcgccgccga tattattgaa atttgatagc tgttcacgct tatggatagt
     2101 gcgctggagc ctgatttgac tcatgaagat ggcgatgccc tggcgcaggc actgggcgcg
     2161 gcgctggagg cggcgggcga cgcgcccgtg cagcacctgc agacccattt gtcgcacctg
     2221 ctgctcacgc ccacatatgc ctacaagctc aagaaaccgc tgcgcctgcc attcgccgac
     2281 ttctccacgc tggccgcgcg ccggcatttc tgcaacgagg agctgcgcct gaaccagcgc
     2341 ctggcgcccc agctgtacct ggacgtgctg cccgtgctgg gcagcgtgcg ccagccgcgc
     2401 atcggtagcg cacacgaggc cggcgccgcc atcgactggg tgctgcgcat gcgccgcttc
     2461 gcccctggca gcgagctcga tgccctggtg cgcgccggcc agctgcaggg ctttgagctg
     2521 gatgcctttg ccgcgcgctt ggcgcacttt cacgcgcagg cgccggtggc ggggcaggac
     2581 agcgactggg gccggccagc ccaggtgggc cagacgatcg ccgatgtgct cgatcagctg
     2641 gcggcgctgc tcagcggggc cgaggccgca cgcctggctg cgctgcgcgc ctggtttggc
     2701 gcgcgccagc cggccctggc tgggcattgg gccgcgcgcc gcgccgccgg ccaggtgcgc
     2761 gaatgccatg gcgacctgca cctgggcaat gtggtgcgga tcgacggcga gatcaccgcc
     2821 tttgactgcc tggagttcag cccggcgctg cgctggatag acacgctggc cgacgccggt
     2881 tttctgacca tggacctgca cgcccatggc cggcccgacc tggcctggcg ctttctcgac
     2941 gcctacctgg ccgccagcgg cgactacgcc ggcctggccg tgctgcgccc ctacgaggtc
     3001 taccgcgccc tggtgcgcac cctggtcgcg cggctgcgcg ccagtcaggg gggcgcggcg
     3061 caggggccgg actacctggc ctgcgccgag cggctggccg cccccctggg cgcgcgcctg
     3121 ctcatcacct acggactgtc gggctcgggc aagtccacgg cggccgcgca gctcttgcaa
     3181 cagggggcgg tgcggctgcg ctcggacgtg gagcgcaagc gcctgcacgg cctggcgccg
     3241 ctggacgatt cggccgccct gggcctggac atctacacca gcgaggcctc acgccgcacc
     3301 ttcgcgcagc tggccgcgca ggcgcgcgcc ctgcttgcgg ccggttatcc ggtgatcgtg
     3361 gacgcggcct ttctgcgccg gcgcgagcgc gacgcctttc acgcgctggc gcgcgagctg
     3421 ggcctgccgt tcgccatcct gcactgccat gccgacccgg accggctgcg cgagcgcgtg
     3481 cgccagcgcc gcgcggcggg cggcgacccc tccgaggccg acctggccgt gctcgcgcgc
     3541 cagcagcagc aggccgaacc cctggcgccc gaagagcagg agcgcacgct ggatctggcc
     3601 accgatcagg cctgggacgc gcccgccctg taccgccgct ggctgtccgt ccaggcttga
     3661 tgtttgtcaa tacaaacggc ggcaccggtg gcatgctgga gccatgaaca ctatcgccct
     3721 caccgattcc caacgcgagc aactgcgcag cgccctggag ttgcgcaagg agcagctgct
     3781 gcaggagctg gacgccgtcc agcgcgacac cctggccgtc gcctcggccg ccagccgtgg
     3841 ccaggccgat cccgccggca gcccgcgtga ccaggccaac agcatggccg ccgacatggt
     3901 gcgcgacgcc gaggccgcgc gcgaccatgc cgagctgacg gccgtgcgcg ccgcgctgga
     3961 gcgcctggcc gatggcagct atggcgaatg caccgactgc ggccagagcg tcggcgtgcc
     4021 gcgcctgctg gcccagccgt cggccgcgcg ctgcatcgcc tgccagtcca aggccgagac
     4081 ccaggtcagg cgctgagccg cgtgtggtgg ggtggccagc accgcaccag aagctcccag
     4141 gccgggggct tcagccacct tgatcaccgg ggcacggctt ggtggggtag tctatgtgct
     4201 attaattaac tagctatcag cgctttatgc aagcgcgcta aaggcctgtt ttatctatat
     4261 tctgcgcggg cttggcgcca gcagcaccac cagcgctccc gcgccgccgt cggcgggcct
     4321 ggcctgcacg aaggccagca cctccttctt ctgcaccagc cagccctgca cgcggccctt
     4381 gagcacgggc gtcttgcccg gcgagcccag gcccttgcca tgcaccacgc gcacgcagcg
     4441 caggccggtg cggtgcgcca ggcgtatgaa ctggcccagg gcctcgcgcg cctcatccac
     4501 gcgcaggccg tgcagatcga gctggcgctg gatgctccac tggcccgcgc gcagccagcg
     4561 cgtcacgtca gggcccatgc ccgtgcggcg aaagctcagc tggtcgtcca cgtccagcag
     4621 ggtgctgacg tcgaactcgt cgctcaacga ttcatgcagc acgcgcgcct cgtccagctg
     4681 gcgctgcacc ggcaggggct cgggcggcac gggggcgggc cagtggcgct cgtgctggcc
     4741 cttgagcggc tgcacggcgc ccacggcgtc ctggaacagg tggcgctcgg cgcgcgcgcg
     4801 ttgctcggcc tcgcggcgca gccgttcgcg ctcggcctgc tgcgcggccc gctcctgcag
     4861 cgtgcggcgc aggggcttga ggtcggcaaa cgattgggct ttgagcgggg cggtcatggg
     4921 taagtgctca ggacactagc gccggttgta ccgcattgcc caccgtcgcc atccgggccc
     4981 aggtattgcg cggcgatgaa ctgcgtgacc tgggcggggt tgaagttgtc gtcgctcacg
     5041 aacaccagca cgcagcggcc ctgcacgggt gggccccagg tcatgccctc gatgttgtcc
     5101 ggggtgaggc ccagggccgc caggtcggcc accagggtct tgggcaccgg gcggtggttg
     5161 gcgggcgtca gggcatccag cgtgagggtg tcgctgccgg cgcgggtgtc gatgcggtac
     5221 aggcgcgcgc caaagccggc gccggcgctg tagctgcgct ccagcaccag caggtggtgc
     5281 gggccgtgcg ccaggatttc gctgacgccg ttgatctgcg ggccgggcag gcggcgccgc
     5341 tgcggcacgg catcgccctg gtaggcaatc tggcgcagcg gctggccgct ggcgatgtcc
     5401 atggcggtga tgcgcacggg tgcgccgcca ctgtcgggcg tggcctgcgg cccgtcctgg
     5461 tgccagggca gctccatggc cagccaggcg gtgcgcccgt cgggcgtgag cgccagcccc
     5521 tccagcgtgc cgttgccgcg cgggccgcgc ctgggttgtg tgccggggga gaagctggcg
     5581 ggcagggcga tgtcgcgcac cggccggccg tccaggcggc tttcgcgcag ctgcgggccg
     5641 aagccgcggc tgaagtcgcc ctcgctgctc cacagaaaac tggtcttgcc cggcagccag
     5701 cgcaggccct cggcgtcggg ccggtccatg cccgcctggc ggcgccacca gggcgcaaag
     5761 ggctggccgc tggtgtgcag cagatcctgg gtggcggtga actgcggcat gtccagccgc
     5821 tgcgcgtcgt agtgcaggcg cgcgcggtag atgcgcgccg atgcgtcact gatcagcagg
     5881 tattcgccgc tgtgcgcgtc gtagtcgatg cccgacaggc cgccgatgcg ggcgccggcg
     5941 tactcgctgc cgtgggccag ctcggccacg ccgatcaggc gcagatgcgg cgccagcagc
     6001 ggcgcgggcg ccggcggcgc cagcggcagg gcgcagccgg ccagcgccag cagggcgatg
     6061 caggcccagc gcgccaggct cacaccaggc cctgctcggc catggacagg gccacgcccg
     6121 gcgccacgat gacatggtcg agcacgcgca cgtccaccag cgccagggcg gccttcagcg
     6181 tctgggtcag ggcctcgtca gcgcggctcg gttgcacgct gccgctgggg tggttgtgcg
     6241 ccagcaccac ggcggcggcc tggtgctgca gggcgcgcag caccacctcg cgtgggtaga
     6301 cgctggtctg cgtgagcgtg ccccggaaga gttcttccat cgccagcagg cggttctggc
     6361 tgtccaggaa cagcacggcg aacacctcat gcgcgcgcgc cgccaggtgc agctgcaggt
     6421 agtgcttgac ggcgcccggc gtgccgaaca cctcgcgctc gcgcagctgc tgggccaggg
     6481 cgcggcgcgc cagctccagc acggccacca gctcggcgcg cttggccggc cccaggccct
     6541 tgatgcgcgc caggtctgcc gcgcccgcgt gcagcaggcc ggccatgccg ccaaagccgc
     6601 cgccggtgat gcgcccgccg gcgtcgcgcc ggggctcatc gagcagctcc tgggccagct
     6661 gcagcacgcc cttgccggcc atgccggtgc gcaacaagat ggccagcagc tcggcgtccg
     6721 acagggcggc ggggccgcgc gcgagcagct tctcgcgcgg ctgggcatca aagggcaggt
     6781 ctttcagcgg catggcgtgg ggcagtgggg gtatacccgg ttttctacaa tggcggcagt
     6841 ttatagagag cttctcaatg accgctgacg cccttgcctc cgacaccagc cagcccatcg
     6901 tgcagatggg ttcctttctt acgctgcatt accgcctggc cggcccggcg ggcgatgtgg
     6961 tcaacacctt cgagggcaag ccggccacgc tgtcggtggg cacgggcgag ctctcgccgg
     7021 cgctggagca gcgcctgatc ggcctggccg agggcgcgcg cgcccgcttc gagctggccg
     7081 ccggcgaggc ctttggcgag cgcaaccccg acatgcggca atgggtgggc cgcaagctgc
     7141 tcaacgacct gggcgatccc gacgagcagt accacgtggg tgacgtggtc gagttcccca
     7201 cgcccgatgg cgcgggcaaa tatgccggcg cggtgctgga ggtgcgcggc gatggggcgg
     7261 tgcgctttga cttcaatcac ccgctggccg gcctgcccgt caccttcgag gtgcagttga
     7321 ttggtgtgct gtaaatgaac aagccccagg aaattgttct ggccgagccg cgcggctttt
     7381 gcgccggcgt ggaccgcgcc atcgagatcg tcgaacgggc gctgcaaaaa tttggccgcc
     7441 ccatctacgt gcgccacgag atcgtgcaca acacctatgt ggtgaacgac ctcaagtcca
     7501 agggcgcgat cttcatcgag gatctggccg aggtgccgcc cggcgccacg ctgatcttca
     7561 gcgcccatgg cgtgagccgc gccgtgcagg acgaggcgcg cgcgcgcggt ttcagcatct
     7621 ttgacgccac ctgcccgctg gtgaccaagg tgcatgtcga ggtcgccaag ctggccaggg
     7681 agggctacga gttcatcatg atcggccaca agggccaccc cgaggtcgag ggcacgatgg
     7741 gccagcttga cagcggcatc cacctggtgg aggacgtggc cgacgtcgcc cgcgtgcagc
     7801 ccgcgcagac cgagaaactc gccgtggtca cgcagaccac cttgagcgtg gacgacgccg
     7861 ccgagatcac cgccgctgtg cgcgcgcgct ttccctccat ccgcgagccc aagcagcagg
     7921 acatctgcta cgccacgcag aaccgccagg acgcggtcaa gctgctgtcg ccccaggtcg
     7981 atctggtgat cgtcgtcggc agccccacca gctccaacag caaccgcctg cgcgagctgg
     8041 ccgcgcgcct gggcaccgcc gcccacatgg tcgacagtgc cgacgaactg cgcgccgaat
     8101 ggttcgacgg catcgcgcgc gtgggcctga cggccggcgc ctcggcgccc gaggtgctgg
     8161 tacaggccgt gatcacgcgc atcaaggagc tgggggcggt gtcggtgcgc aagatgagcg
     8221 gcatcgagga atcggtgaaa ttccccctgc ccaagggcct gaagctggac gccgccacgg
     8281 gcctggagct ggccgagcgc agcccggaga ccggcgcgag cagcggctga acttcgggcg
     8341 gcgccgtgcc aggtgcgcgg cgcaggaggt ggttgcgctt ctgtatttat agctgttgac
     8401 gcgctatgag tgggcgctgc agccaaattt ggctaatata acgaaatgcc gctgatcggg
     8461 cagggcatgt gggccgcagc ggccgaagtc ccgcacaatg cgggacttct tacaattgcc
     8521 gcatcttcac accttgggag cacgtcgggc tccctgcata tggaaatagc aatactgttc
     8581 gccctcatcc tgctcaacgg cgtgtttgcc atgtccgagc tggctttggt ctccgcgcgc
     8641 aagacgcgcc tgcaaaagct gatcgatgag ggcgatagcg gtgccatcgc cgccgtcaag
     8701 ctgggcgaag acccgacgcg ctttctgtcc accatccaga tcggcatcac ctcgatcggc
     8761 gtgctcaacg gcatcgtggg cgagtcggcc ctggccaagc catttggtga atggctgatc
     8821 ggcctggggc tgcaggcgca atacgccagc tacatcgcca ccggcctggt ggtggtgttg
     8881 atcacctact tctccatcgt cgtcggcgaa ctcgtgccca agcgcctggg ccagagccac
     8941 cccgaggccc tggcacgcat cgtggcccgc cccatcaact ggctggccct ggctaccaag
     9001 ccctttgtgc gcctgctgtc catctccacc gagaccctgc tgcgcgccct gggcgtgaag
     9061 cagggccagg gcaacgctgt gaccgaggac gagatccatg ccgtgctggc cgagggcacg
     9121 agcgccggcg tgatcgagtc gcacgaacac cagatggtgc gcaacgtgtt ccgcctggac
     9181 gaccgccaga tcgggtcgct gatggtgccg cgcgccgacg tggtgtgcct ggacgtgctg
     9241 gagtcgttcg
//
LOCUS       DAOMTQ010000121         9144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1556_length_9144_cov_28.051931, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000121 DAOMTQ010000000
VERSION     DAOMTQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1556_length_9144_cov_28.051931"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..458)
                     /locus_tag="PK518_11455"
     CDS             complement(105..458)
                     /locus_tag="PK518_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49575.1"
                     /translation="MRRLLLILLLAFMPLQSIWAAAAGYCSHEETPGAAHFGHHVHQH
                     HEDGDGAKAGTDLAKAALDMDCHACHGMSNALHQDAGATPLWAQDLQLAPPLRFTLPT
                     PSPQRPERPNWRPVA"
     gene            complement(508..2070)
                     /gene="ahpF"
                     /locus_tag="PK518_11460"
     CDS             complement(508..2070)
                     /gene="ahpF"
                     /locus_tag="PK518_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HRM49576.1"
                     /translation="MLDDNLKTQLATYLERVTQPFEMVASLDDGDTSREMRELLTTIQ
                     SLRSDKITLRTDGQDARKPSFTLQRIGSDSSLRFAGLPLGHEFTSLVLALLWTGGHPP
                     KVEQDLIEQVQQLDGDFHFEVYMSLSCHNCPDVVQALSLMAILNPKIKTTVIEGGAFE
                     KEVTEREVMAVPMVYLNGAVFGAGRMELAEIVAKLDTGAAKREAAKLDAKQAFDVLIV
                     GGGPAGAAAAVYAARKGIRTGLAAERLGGQVNDTLDIDNYISISHTEGPQFAAALERH
                     VRDYEVDVMNLQRAKALRPAAAAGGLIEVEMDNGAVLKAKTVILSTGARWRNMNVPGE
                     DQYRTKGVAYCPHCDGPLYKGKRVAVIGGGNSGVEAAIDLAGLVEHVTVFEFMPQMKA
                     DAVLQKKLASLPNVEVILNAQTTEVLGDGSKVTGLAYKDRATDEAKQVELAGIFVQIG
                     LLPNTDWLKGAVELSKFGEIVIDAKGQTSAPGVFAAGDCTTVPYKQIVIAAGAGATAA
                     LSAFDHLIRTSA"
     gene            complement(2168..2743)
                     /gene="ahpC"
                     /locus_tag="PK518_11465"
     CDS             complement(2168..2743)
                     /gene="ahpC"
                     /locus_tag="PK518_11465"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HRM49577.1"
                     /translation="MSLINTQVQPFKTTAFVNRGGKGEFIEVTEASLKGKWSVLIFMP
                     AAFTFNCPTEIEDAAEHYAEFQKAGAEVYIVTTDTHFSHKVWHETSDAVRKAHFPLVG
                     DPTHQLTNAFGVHIAEEGLALRGTFVVNPDGVIKTMEVHSNEIARDVAETLRKLKAAQ
                     FTAAHPDQVCPAKWKEGAKTLTPSLDLVGKI"
     gene            2947..3423
                     /gene="bfr"
                     /locus_tag="PK518_11470"
     CDS             2947..3423
                     /gene="bfr"
                     /locus_tag="PK518_11470"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRM49578.1"
                     /translation="MKGDAQAIAHLQAQLKNELTAINQYYVHYRMLKHWGFDRLAKKE
                     YEESIGEMKHADKLMERIFMLDGLPNLQDLAKLQIGEDVPEILECDLRAERGAQTTIK
                     EGIAYCESVRDYVSRDLLQDILEDTEEHIDFLETQIELVDKVGLHNYLQSQMGDAD"
     gene            3650..3979
                     /gene="flhD"
                     /locus_tag="PK518_11475"
     CDS             3650..3979
                     /gene="flhD"
                     /locus_tag="PK518_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HRM49579.1"
                     /translation="MTSEQLLAEIREANLTYLMLAQTLIRQDKAEAVFRLGINEDAAD
                     ILASLSAAQVLKLASRNTLLCGFRVDDDLVWSLLTNHNTPRKSINEATNTLHANILMA
                     SRVSEVL"
     gene            4024..4563
                     /gene="flhC"
                     /locus_tag="PK518_11480"
     CDS             4024..4563
                     /gene="flhC"
                     /locus_tag="PK518_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HRM49580.1"
                     /translation="MSAPVKSVLNESKQIERAAMLIEMGARMQVLESETTLSYERLIR
                     LYKEIAGKSPSKGQLPFSTDWFLTWQENIHSSLFLNIYEYLSKGVSLDSVELLTKAYR
                     LYNEQVAAAEIEPLLSFTRAWRLIKFVDAGMLTRTKCSQCGGQFVTELYENRHYTCGL
                     CNPPARAGKSKSAGALTLH"
     gene            complement(4571..5845)
                     /locus_tag="PK518_11485"
     CDS             complement(4571..5845)
                     /locus_tag="PK518_11485"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HRM49581.1"
                     /translation="MKSIAVIGGGITGVTSAYALARRGFSVTLFEQHRYAAMDTSFAN
                     GGQLSASNAEVWTHGSTILKGLKWMLRGDAPLLVNPRPTWHKLSWFAEFIAHIPKYRE
                     NTIATARMAIAARQHLFAWAEAEGVEFDLKKQGILHIYRDRKGFDHAGEVSRLLAAGG
                     LERRAVTPEEMRQIEPTLAGDFFGGYFTESDSTGDIHKFTTGLAAAFERHGGRCLFGA
                     LVKGVASNSGGATVDFECDGVTERRHFDAMVVCAGIGSRALATQLGDRINIYPVKGYS
                     ITVNLLDQPSQQAAPTVSLLDDETKLVTSRLGQDRFRVAGTAEFNGANRDIRADRIQP
                     LINWVNQCFPDVSTRQVVPWAGLRPMTPSMLPRVGPGARPNVFYNTGHGHLGWTLSAA
                     TADMLADMVCAASRACSASVVAVGQAPLAQEV"
     regulatory      complement(6001..6098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(6167..6709)
                     /locus_tag="PK518_11490"
     CDS             complement(6167..6709)
                     /locus_tag="PK518_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HRM49582.1"
                     /translation="MKRRAALAAAVLACACALQGCAQYYYGDRAAVAPVDLVASNHGA
                     ADALLEQVALNAEQPVLVSTVVQLDRLGESSRLGRLISEQLAGRLARRGLRVTELRLR
                     ETLALRPRQGELLLSREVGEVSQAQAAQAVLVGTYAAASQAVYVSLKLVSPHGNAVLA
                     AHDYALPMDGNVRGLLVGEY"
     gene            complement(6706..7434)
                     /locus_tag="PK518_11495"
     CDS             complement(6706..7434)
                     /locus_tag="PK518_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49583.1"
                     /translation="MFSSIHRASVHAAVLAATLLAGCSNLDVPRADNYPATGQKKARA
                     VHHWDVLADDVAARVAEKTAAWPEGGQPIHVTVADNSSFNQGFLKLLRVRLLNKGVAL
                     STGPSALELEVQTQLVQHQAGVPSNLPIPTPWTLLGSGVGVWRDWETHYSDRVLLPGV
                     ATAIGLGAGLAMDLAQRHTQGAAAGGPTRTEVLITTTLKTQDRYLAGSADMYYIERAD
                     AALYQPELPPPAPAPLRTWKVVAP"
     gene            7587..8795
                     /locus_tag="PK518_11500"
     CDS             7587..8795
                     /locus_tag="PK518_11500"
                     /inference="COORDINATES: protein motif:HMM:NF023560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spherulation-specific family 4 protein"
                     /protein_id="HRM49584.1"
                     /translation="MTTMHTTSHIASLLALTAALAGCGGGTEKSTAPTTPAPPPPSGI
                     VSLSFITNAQANVFDRGTLSSALNVQAGLDGSNANNLTVTFTTTAGSVSPVQVATNSS
                     GLAATSLSIPTATPTGAVRITATAAANNTAAATFAGYVRAVPNRLELLVPAYFSAGSN
                     AAAWTALTDGATSYPDVAMNVIVKPDNASNGTMTAAITKADDKLITAITALKTAQPRA
                     KVFGYVATGGGANAAISSTDVSVTIDRYIALYPGQLDGIFLDGMATDDSRRAFFQGIY
                     AYANDKGLKVIGNPGAYPAPSYAGLADMLVTYTGPATSYQGADPQPANTWVYDRQNTA
                     QGMLVHTASTCSEMQKLVTDANRPRMNTGLVYVTDQAIGSPWSALPSYWKNLLGTVDA
                     TNKGLARPVC"
     gene            complement(8808..>9144)
                     /locus_tag="PK518_11505"
     CDS             complement(8808..>9144)
                     /locus_tag="PK518_11505"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRM49585.1"
                     /translation="MVLAKSDLALASRYSELVPDARLRKKVFAAIEQEWQRTAEALTR
                     ITGEKQRLAANTALARSIRHRFPYIDPLHHLQVELIRRWRTGQGDERVQTGIHICING
                     IAAGLRNTG"
BASE COUNT         1667 a         3055 c         2844 g         1578 t
ORIGIN      
        1 ggcgcgaatc catcgctttc attgtgttcc tctacttcca ggttgccgta ctaggccact
       61 ggagcgatca agagggaaag ggctgccctg ccccgccttg ccgttcaggc aacggggcgc
      121 cagttggggc gttcgggccg ctgagggctg ggggtgggca gcgtaaagcg caggggcggc
      181 gccagctgca ggtcttgcgc ccacaggggc gtggcgccgg cgtcctggtg cagggcgttg
      241 ctcatgccat ggcaggcgtg gcagtccatg tcgagcgcgg ccttggccag gtcggtgccg
      301 gccttggcgc cgtcgccatc ctcatggtgc tggtgcacat ggtggccgaa atgcgcggcg
      361 ccgggagtct cctcatggct gcaatagcca gccgccgcgg cccagatgga ctgcagcggc
      421 atgaaggcca gcaagaggat gagcaggagg cggcgcatga agagcgcgca ttctataacg
      481 cgccctccat ggcttcaggc cagggcttca ggccgaggtg cggatcaggt gatcaaaggc
      541 gctgagtgcc gccgtcgccc ctgccccggc ggcaatgacg atctgcttgt agggcaccgt
      601 ggtgcagtcg ccagcggcga acacccccgg cgcgctggtc tggcccttgg cgtcgatgac
      661 gatctcgccg aacttggaca gctcgaccgc acccttgagc cagtcggtgt tgggcagcaa
      721 gccgatctgc acaaagatgc cggccagctc gacctgtttg gcctcgtcgg tcgcgcggtc
      781 tttgtaggcc aggccggtga ccttgcttcc gtcgcccagc acctcggtgg tctgggcgtt
      841 caggatgacc tcgacgttgg gcaggctcgc cagctttttc tgcagcacgg cgtcggcctt
      901 catctgcggc atgaattcga acaccgtcac atgctcgacc aggccggcca ggtcgatggc
      961 cgcctcgacg ccggagttgc cgccgccgat caccgccacg cgcttgccct tgtagagcgg
     1021 gccgtcgcaa tgcgggcagt aggccacgcc cttggtccgg tactggtcct cgccgggcac
     1081 gttcatgttg cgccagcgtg cgccggtcga gaggatcacg gttttggctt tcagcaccgc
     1141 gccgttgtcc atctcgacct cgatcaggcc acccgctgcg gcggccggcc tcaaggcctt
     1201 ggcgcgctgc aggttcatca catcgacctc gtagtcgcgc acatggcgct ccagcgccgc
     1261 ggcgaactgc gggccctcgg tgtgcgagat cgagatgtag ttgtcgatat cgagcgtgtc
     1321 gttgacctgg ccgcccaggc gctcggccgc cagacccgtg cgaatgccct tgcgcgccgc
     1381 gtacacggcc gccgccgcgc cggccgggcc accgccgacg atgagcacgt cgaacgcctg
     1441 cttggcgtcg agcttggcgg cctcgcgctt tgccgcgccg gtgtcgagct tggcgacgat
     1501 ctcggccagc tccatgcggc cggcgccgaa caccgcgcca ttcaaataga ccatgggcac
     1561 ggccatgacc tcgcgctcgg tgacttcctt ctcgaaggcg ccgccctcga tcaccgtggt
     1621 cttgatcttc gggttcagga tggccatcag cgacagcgcc tgcaccacgt cagggcagtt
     1681 gtggcaggac aggctcatgt agacctcgaa gtggaagtcg ccgtcgagct gctgcacctg
     1741 ttcgatcagg tcttgctcga ccttgggcgg gtggccgccg gtccacagca gggccagcac
     1801 caacgaggtg aattcatggc ccaaaggcag gccagcaaag cgcaggctgc tatcgcttcc
     1861 gatgcgctgc agcgtgaacg agggcttgcg tgcgtcctgg ccgtcggtgc gcagcgtgat
     1921 cttgtcgctg cgcagggatt gaatcgtcgt cagcagctcg cgcatctcgc gcgaggtgtc
     1981 gccgtcgtcc agggaagcca ccatctcgaa cggctgcgtc acgcgctcca gataggtggc
     2041 gagttgggtt ttcaggttgt cgtcaagcat ggtggattcc tttatgtgtt ctagaagttc
     2101 agggcgtgaa aaagccggac ggcagggcgc atgcgcccca catctgtccg gctgtgcggg
     2161 cggcggctta gatcttgccg accagatcca gcgacggcgt cagcgtcttg gcgccttcct
     2221 tccacttggc cgggcacacc tggtcaggat gcgctgcagt gaactgggcc gccttgagct
     2281 tgcgcagggt ttctgccacg tcacgggcga tttcgttgga atgcacttcc atggtcttga
     2341 tcacgccgtc ggggttgacc acaaaggtgc cgcgcagcgc caatccttct tcggcaatat
     2401 gcacgccaaa ggcgttggtc agctggtgcg tggggtcgcc caccagcggg aaatgggcct
     2461 tgcgcacggc atccgacgtc tcgtgccaga ccttgtgcga gaaatgcgtg tcggtggtga
     2521 cgatgtagac ctcggcgccg gccttctgga actcggcgta atgctcggct gcgtcctcga
     2581 tctcggtcgg gcagttgaag gtgaaggccg cgggcatgaa gatcagcacc gaccacttgc
     2641 ccttgaggct ggcttcggtg acttcgatga attcgccttt gccgccacgg ttgacgaagg
     2701 cggtggtctt gaagggttga acttgcgtgt tgatcaggga catcgttgtt tcctttcggt
     2761 tgggttggaa gtgataacgg actgaagtct agttgcttgg cgacaatcaa tccaatcaat
     2821 tgaattcatt ggtttgattg ttcaaatcta taaaagttcc ccggcacaca ttccggcccg
     2881 catgtctcca ggcccatgcg aagtggatca caatgcggct cttcccccga caaccaggag
     2941 ccccccatga aaggcgacgc acaagccatt gcccacctgc aggcccagct caagaacgaa
     3001 ctcaccgcga tcaaccagta ctacgtgcat taccgcatgc tcaagcactg gggtttcgac
     3061 aggctggcga agaaggagta cgaagagtcg atcggtgaga tgaagcatgc cgacaagctg
     3121 atggagcgca tcttcatgct cgacggtctg cccaatctac aagacctggc gaaattgcag
     3181 atcggagagg atgtgcccga gatcctggaa tgtgacctgc gcgcggagcg cggcgcacag
     3241 accaccatca aggagggcat cgcctactgc gagtcggtgc gcgactatgt ctcgcgcgac
     3301 ctgctgcagg acattctgga ggacaccgag gagcacatcg attttctgga aacgcagatc
     3361 gagctcgtgg acaaggtggg cctgcacaac tacctgcagt cgcaaatggg cgatgccgac
     3421 tgacaccgaa cctgcctcac tacacaacgg gctgcggccc gtttttttac gcccgcatgc
     3481 cgacagcgat gtaaggcaaa ttcctacaaa gggcgtcaga caagcggacg gcacgcagcg
     3541 ccgaaccccg acttcatttt cttttggccg ggtttcacaa tccatttcaa cggatcacgc
     3601 aacacatgca accagttgca agcgatccac cactggaaag gattgaccca tgacctccga
     3661 acaacttctc gccgaaatcc gcgaagccaa cctcacttac ctgatgctgg cccagaccct
     3721 gatccgccag gacaaggccg aagccgtgtt ccgtctgggc atcaacgaag atgcggccga
     3781 cattctggca tcgctgtccg cggcccaggt gctcaagctg gcctcgcgca acacgctgct
     3841 gtgcggcttc cgcgtggacg acgacctggt gtggagcttg ctgaccaacc acaacacgcc
     3901 gcgcaagtcg atcaacgagg ccacgaacac gctgcacgcc aacatcctga tggccagccg
     3961 tgtctccgaa gtgctctgag ccaagccccc cctgcaccgc ccccaagcca acgaaagccc
     4021 gccatgtccg cacccgtcaa gagcgtcctc aacgagtcca agcaaatcga acgcgccgcc
     4081 atgttgatcg agatgggcgc ccgcatgcag gtgctggagt ccgagaccac gctgtcgtac
     4141 gagcgcctga tccgcctgta caaggagatc gcgggcaagt cgccctccaa gggccagctg
     4201 ccgttttcca ccgactggtt cctgacctgg caggagaaca tccacagctc gctgttcctg
     4261 aacatctacg agtacctgtc caagggcgtg agcctggaca gcgtggagct gctgaccaag
     4321 gcctaccgcc tgtacaacga gcaggtggcg gccgccgaga tcgagcccct gctgtccttc
     4381 acgcgcgcct ggcgcctgat caagttcgtc gatgccggca tgctcacgcg caccaaatgc
     4441 tcgcaatgcg gtggccagtt cgtcaccgag ttgtacgaga accgccacta cacctgcggc
     4501 ctgtgcaacc cgccggcacg cgccggcaag agcaagagcg caggtgcatt gaccctgcac
     4561 tgataaaaga ctagacctcc tgcgccagcg gcgcctggcc cacggccacc acgctggcgc
     4621 tgcacgcacg gcttgcggcg cacaccatgt cggccagcat gtccgccgtc gcggccgaca
     4681 gcgtccagcc caggtggcca tggccggtgt tgtagaacac gttcggccgg gcgcccgggc
     4741 ccacgcgcgg cagcatgctc ggcgtcatgg gacgcaagcc ggcccagggc acgacctggc
     4801 gcgtgctgac atccggaaag cactggttca cccagttgat cagcggctgg atgcggtcgg
     4861 cgcgaatatc gcgattggcg ccattgaact ccgcggtgcc agccacccgg aaccgatcct
     4921 gtcccaggcg gctggtgacc agcttggtct catcatcgag caggctcacg gtgggcgcgg
     4981 cctgctggct cggttgatcc agaagattga cggtgatgga atagcccttg accgggtaga
     5041 tgttgatgcg atctcccagc tgcgtggcca gcgcacggct gccaatgcct gcacagacga
     5101 ccatggcgtc gaaatgccgg cgctccgtga cgccgtcgca ctcgaagtca acggttgcgc
     5161 ccccgctatt gctggccacg cccttgacca gcgcgccaaa caggcagcgc ccaccatggc
     5221 gttcaaacgc cgcagccagg ccggtggtga acttgtggat gtcgccggtg gagtcgctct
     5281 cggtgaagta gccaccaaag aaatcgcccg ccagcgtggg ttcgatctgc cgcatttcct
     5341 cgggcgtcac ggcacgccgc tccagtccac cggcggccag cagcctggag acctcaccgg
     5401 catgatcgaa gcctttgcga tcacgataga tgtgcaggat gccctgcttt ttcagatcga
     5461 actccacgcc ctcggcctcg gcccaggcaa acaggtgctg gcgtgcggca atcgccatgc
     5521 gtgccgtggc aatggtgttc tctcgatatt tcggtatgtg ggcaatgaac tcggcaaacc
     5581 acgacagctt gtgccaggtc gggcgcgggt tgaccagcag cggcgcatcg ccgcgcagca
     5641 tccacttcag gcccttgagg atggtcgagc cgtgcgtcca gacctcggca ttcgaggcag
     5701 acagctgacc gccattggca aacgaggtgt ccatcgccgc atagcgatgc tgctcgaaca
     5761 gggtgacgga aaagccgcgc ctggcaagcg cgtaggccga ggtaacgccg gtgatgccac
     5821 cgccgatgac agcgattgat ttcatgaaag ctccaaacgt cggaagggtg gagtgcgacg
     5881 gcaacatgca tgccgcacgc cccctctgtt tggaacctga gagattcgca cccgcgccgc
     5941 tgcggggtgc ttgctcctgc ggtggggagg atgacgaatc cgcccgctct tcagagtgtc
     6001 atgaactggc cggtcctttt gcctgagagt ttccggggcg gttgctcctt cggcgccact
     6061 gggttgggat gcccagcggt ctctcccgac caatgcgcgc gatcatagcg ccgtttgcag
     6121 ccgtggcggc cttgccccat gcatccaagg cctgccgacg atcgcttcaa tattccccga
     6181 ccagcagacc gcgcacattc ccatccatgg gcagcgcata gtcatgcgcc gccagcacgg
     6241 cattgccatg cgggctcacg agcttgaggc tcacgtatac cgcctgagag gcggccgcat
     6301 aggttcccac cagcacggcc tgggcggcct gcgcctggct gacctcgccc acctcgcgcg
     6361 agagcagcaa ctcaccctgg cgtggccgca gggccagcgt ctcgcgcagg cgcagctcgg
     6421 tcacgcgcag gccgcgccgg gccaggcggc cggccagctg ctcggagatc aaacgcccca
     6481 ggcgcgagga ttcgcccagc cgatcgagtt gcaccacggt ggacacgagc acgggctgct
     6541 cggcattcag cgccacctgc tccagcaagg catcggcagc cccatgattg ctggccacca
     6601 ggtcaaccgg cgccactgcg gcccggtcgc cgtagtaata ctgcgcgcaa ccctgcaacg
     6661 cgcaggcgca tgccagcaca gcggcagcca gggccgcacg gcgtttcatg gcgccaccac
     6721 cttccaggtg cgcagcggcg caggcgccgg cggcggcagc tcgggctggt agagcgccgc
     6781 atccgcacgt tcgatgtagt acatgtctgc cgaccccgcc agatagcgat cctgggtctt
     6841 gagggtggtg gtgatgagca cctcggtgcg cgtggggccg cccgccgcgg cgccctgggt
     6901 gtggcgctgc gccagatcca tggccagacc cgcccccagg ccgatggcgg tggccacgcc
     6961 tggcagcaag acacgatcgg agtaatgcgt ttcccaatca cgccacacac ctacgccgct
     7021 gcccagcaga gtccagggcg ttggaatagg caggttgctt ggaacccctg cctggtgctg
     7081 caccaattgc gtctgaacct ccagctccag cgccgacggc ccggtggaca gtgccacccc
     7141 cttattgagc aggcgcaccc gcagaagctt aagaaagccc tggttgaaac tggagttgtc
     7201 ggctaccgtg acatggatgg gctgtccacc ctcgggccag gcggccgtct tctctgccac
     7261 gcgtgcggcc acgtcgtcgg ccagcacatc ccaatgatgc acggcacgcg ccttcttctg
     7321 cccggtggcc gggtagttgt cggcgcgcgg gacgtccagg ttgctgcaac cggccagcag
     7381 agtcgccgcc agcacagccg catgaaccga ggcgcggtgg atggatgaaa acatgatccc
     7441 gatgataggg atggagtccc ggggcaatcg acacaacaac gccgggtttc ccgttcaaag
     7501 cggcggtttc gatgcctcaa gcactattag catgcggtca caacagcaac atgaatgcaa
     7561 caccgcggga atccaccttc ctcctcatga ccaccatgca caccacatcg cacatcgcgt
     7621 ctctgctggc cttgactgcc gccttggcgg gctgcggcgg cgggacggag aaatccaccg
     7681 ccccaaccac gccagcaccc ccaccgccgt cgggcatcgt cagcttgagc ttcatcacca
     7741 acgcccaagc gaacgttttc gacaggggca cattgagcag cgcactgaac gtccaggcag
     7801 gcctcgatgg cagcaacgcc aacaacctca cggttacgtt cacgaccacc gccggcagcg
     7861 tcagcccggt gcaggtcgcc acgaacagca gcgggctggc agccacgtcg ctcagcatcc
     7921 ccaccgcgac gcccacaggc gccgtgcgca tcacggccac ggccgcggcc aacaacaccg
     7981 ccgccgccac ctttgccggc tatgtgcgcg ccgtgcccaa caggctggaa ctgctggtgc
     8041 ccgcctactt cagcgccggc tccaatgcgg cagcctggac ggcactcacc gacggcgcca
     8101 ccagctaccc cgatgtggcg atgaatgtca tcgtgaagcc ggacaacgcc agcaacggca
     8161 ccatgaccgc cgccatcacc aaggccgacg acaagctcat cacggccatc accgcgctca
     8221 agaccgcgca accccgcgcc aaggtgtttg gctacgtggc caccggcggc ggtgccaatg
     8281 cggcgatttc aagcaccgac gtctccgtca ccatcgacag gtacatcgcc ctctaccccg
     8341 ggcaacttga cggcattttt ctggacggca tggccaccga tgacagccgc agggcctttt
     8401 tccagggcat ctacgcctac gccaatgaca aggggctgaa ggtcatcggt aaccccggcg
     8461 cctacccggc ccccagctat gccggcctgg ccgacatgtt ggtgacctac accgggcccg
     8521 ccaccagcta ccaaggcgcc gacccccagc ccgccaacac ctgggtctac gacaggcaga
     8581 acaccgccca gggcatgctc gtgcataccg ccagcacctg ctccgaaatg cagaagctgg
     8641 tgaccgacgc caaccggccg cgcatgaaca cgggcctggt gtacgtcacc gaccaggcca
     8701 tcggcagccc ctggtctgcc ctgcccagct attggaagaa cctgctgggc accgtggacg
     8761 caaccaacaa gggccttgcg cggccggttt gctgatggtg cgcaagctca cccggtattg
     8821 cgcaaccccg ccgcaatgcc gttgatgcag atgtggatgc ccgtctgcac ccgctcgtcg
     8881 ccctggccgg tgcgccagcg gcgtatcagc tcgacctgca ggtggtgcag cgggtcgatg
     8941 tacggaaagc ggtggcgtat cgagcgtgcc agcgccgtgt tggccgccag gcgctgcttt
     9001 tcgccggtga tgcgcgtcag cgcctcggcc gtgcgctgcc attcctgctc tatggccgcg
     9061 aacaccttct tgcgcaggcg cgcgtcgggc accagctcgc tgtagcgcga ggccagggcc
     9121 aggtcgctct tggccagcac catg
//
LOCUS       DAOMTQ010000122         8888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1644_length_8888_cov_28.004415, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000122 DAOMTQ010000000
VERSION     DAOMTQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_8888_cov_28.004415"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PK518_11510"
     CDS             complement(<1..713)
                     /locus_tag="PK518_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRM49586.1"
                     /translation="MENKAHALAAGIFVIAVAALLAGLAMWLTRDQTDYRVYELSSKE
                     SVSGLQPQAAVRYKGVAVGKVRSIGFDPQAVGHVLIRIAVNTDAPIADTTFATLGYQG
                     VTGLAHIQLDDADAPLPQPAPGASGLPRLPLQSSPLSQLAEQGPAILGQVQDVMARLG
                     TLLGEDNQQHFGQALQHLGAAAASIDQLVRRLDHTVSTRLDPALAGMPALTQDAQQTL
                     QALRQAGASAAGAADEVNQA"
     gene            complement(713..1516)
                     /locus_tag="PK518_11515"
     CDS             complement(713..1516)
                     /locus_tag="PK518_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM49587.1"
                     /translation="MADADTIIEIRDLATVFGQGDSAFTVHDQLQLTVRRGEILALVG
                     GSGTGKTVLLRHILGLTRPTRGSVTVLGRPAAELGGEGASARVGMLFQHGALYSAFNV
                     LDNIAFALRELGTLPPALVQDVAMVKLRLAGLKAEHATRMPADLSGGMIKRVALARAL
                     IMDPPLLLLDEPTAGLDPFSSDEFCELLRELHAALGLTVIMVTHDLDTLFALSTRVAV
                     LADRHVITSGTAQQVAQFDHPFIHHYFAGERGRRAMAQPPDPAAAPQEA"
     gene            complement(1519..2637)
                     /locus_tag="PK518_11520"
     CDS             complement(1519..2637)
                     /locus_tag="PK518_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49588.1"
                     /translation="MPVPSPPTIVQDSTATPCALPQGDWVAAAFAERQAWRRVAGELK
                     ACGPGHAWNLRDLARLDHVGAQILWNHWGRAWPGQIELSDTQRQMLERVAQFSTPCAA
                     PAPWRLSTQIDRLGELVLHALDHLTGLVLLVGQLLLDMLRLAAKPLRAPWRDISGHLY
                     QMGARALPITALVGFLIGVVLAYLMSLQLRQFGAEAFIVNILGISLIRELGPMLGAIL
                     VAGRSGSAITAQIGVMRVTEELDAMRVMGIAHGFRLVLPRALALGLAMPLIALWTTLA
                     ALAGGMLAADLTMGISAAYFLQVLPAAVQVSNLWLAMAKSVVFGVFIALIGCHWGLRV
                     KPNTQSLGEGTTASVVSAITMVIIVDAVFAVAFRSVGF"
     gene            2882..4576
                     /locus_tag="PK518_11525"
     CDS             2882..4576
                     /locus_tag="PK518_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRM49589.1"
                     /translation="MSDTTFDYIIIGGGTAGALLANRLSADPQARVLLVEAGRKDDYH
                     WIHIPVGYLYCIGNPRTDWLYNTEPDAGLNGRSLRYPRGKTLGGCSSINGMIYMRGQA
                     RDYDQWADITGDDAWRWSNVLPAFRRHEDHWRLDEPGSASEAFQQLHGNKRTGSTGEW
                     RVDKQRLRWDILDAFAQAATEAGIPATDDFNRGTNEGVGYFEVNQKDGWRWNTAKAFL
                     RPTCYGRPNFELWTSAQATRVLTEKQADGSLRCTGVQVWTGEEMVTAHAQQEVILSAG
                     AVNSPQILQLSGMGPAALLRQHGIDVRLDLPGVGANLQDHLQIRAVYKVQNVATLNTL
                     ANSLVGKAKIGLEYLLKRSGPMSMAPSQLGAFTRSRPDLPWPNIEYHVQPLSLEAFGE
                     PLHDFPAFTASVCNLNPTSRGSVTIKNADFRSAPAIAPNYLSTPEDRQVAADSLRITR
                     HIVAQPALARYRPEEYKPGPQYQSDEELARLAGDIATTIFHPVGTTRMGRMDDPMAVL
                     DSRMRLLDGRGGAVAGLRVVDAGAMPSITSGNTNAPTLMMAEMAAQWIIEASRAKR"
     gene            4832..5590
                     /locus_tag="PK518_11530"
     CDS             4832..5590
                     /locus_tag="PK518_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRM49590.1"
                     /translation="MEWIIVSLASLLAGFVDAIVGGGGLILLPALFATFASAPPATLL
                     GTNKAAAFWGTSMATWQYSKRVQIPWRAMLPGVAAGFVGSFIGAWAVTQMSPDFLRKL
                     LPLVMVAVLAYTLAKKDLGLQHAPRFSGRTERLIVGTIGLVIGLYDGFFGPGTGSFFV
                     FAFVRLLGYDFLNASVSAKLLNLATNVSAIILFAAKGHIWWHFMVPLAVANVLGSMLG
                     TYMALRHGTGFVRAIFILVVGALILKTGYDAFLR"
     gene            5605..6516
                     /gene="rocF"
                     /locus_tag="PK518_11535"
     CDS             5605..6516
                     /gene="rocF"
                     /locus_tag="PK518_11535"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRM49591.1"
                     /translation="MTELTLIGAPTDVGASVLGASMGPDALRIAGIARALQGLGLAVQ
                     DLGNLSGPGNPQAAPQGGFRHLVEVTRWNEAVFAATLSALQAGRLPLLMGGDHCLAIG
                     SISAVARHCSARGRRLKVLWFDAHADANTPASSPSGNLHGMPVACLLGHGPAQLTQLA
                     GAAAALQAQQITLVGVRSVDATEKSFVNTLGIEVYDMRSIDELGMRAVMQAALADVDE
                     NTHLHLSFDMDGLDPTIAPGVGTPVRGGPTYRETQLFMEMLADSGALGSVDIMELNPA
                     LDLRNQTAELAVDLLESLFGKSTLMRA"
     gene            6713..7474
                     /locus_tag="PK518_11540"
     CDS             6713..7474
                     /locus_tag="PK518_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM49592.1"
                     /translation="MKMLKTLAAALAVCAAVTVQARPLEDIQKSGTIILASEGQYAPF
                     NFFQGKQLTGYEIEVGEAVAKKMGLKFEWKTVGFDALLTGLAQDRWDLVIASHAITEE
                     RAKAVTFTAPHYCSGGQIVSMTPAIAKAADLAGKTVAVQTGTTYLDAVKKVPGVKEVK
                     NFPTDDAARNALGSKRVDAWVTDRFVAKEMISKLPKAGFKIGDMLFIEQVASAVTKGN
                     QGLADAWNKALKELIADGTVGKISQKYFQEDVTCK"
     gene            7514..8296
                     /locus_tag="PK518_11545"
     CDS             7514..8296
                     /locus_tag="PK518_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM49593.1"
                     /translation="MLLALWPDHWSRSQRSNATLVAALVLMALVLSLLGQLLSFFPEP
                     IGSNAALFADGALTTLYLTLVSGAAGLVLGTGAALARTSRLAWLRYVASFYIWAIRGT
                     PLLVQILFVYFALPVLVPGLNLPDFAAAVVALGLNVGAYNAEAIRAGLLAVPRGQTEA
                     ARALGLGHGHVFLDVVFPQAFKIALPPLVSNFVALLKDSSLAYAIGVVELTNVGNRIQ
                     SATFQPVATLTTVAITYLILTTLVTQVTHAVEYRFDVEGRAK"
     gene            8293..>8888
                     /locus_tag="PK518_11550"
     CDS             8293..>8888
                     /locus_tag="PK518_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM49594.1"
                     /translation="MSKAYIVCRDVRKRFGEHEVLKGVSTEFQTGQVTVIIGASGSGK
                     STLLRAINRLEPHDSGSITIDGVEVSDDQRTLQKQRSEVGMVFQQFNLFGHLSVLDNV
                     TLAPRRIRRTPRTAANEQAMVLLKRVGMQDHAHKYPWQLSGGQQQRVAIARALAMQPK
                     VMLFDEPTSALDPEMVQEVLDVMRELARGGMTMIVVTH"
BASE COUNT         1491 a         3106 c         2951 g         1340 t
ORIGIN      
        1 gcctggttca cctcgtcggc cgcgccggcg gcgctggcgc cggcctggcg cagcgcctgc
       61 agggtctgct gcgcgtcctg cgtcagcgcg ggcatgccgg ccagggccgg atccaggcgc
      121 gtgctgacgg tgtgatccag gcgccgcacc agctggtcta tgctggcggc ggcggcgccc
      181 agatgctgca gcgcctggcc aaaatgctgc tggttgtcct cgcccagcaa ggtccccagg
      241 cgcgccatca cgtcctgcac ctggcccagg atggccgggc cctgctcggc cagctggctc
      301 agcggcgagc tctgcagcgg caggcgcggc aggccgctgg cgccgggcgc gggctgcggc
      361 agcggcgcat cggcgtcgtc cagctggata tgggccaggc cggtcacgcc ctggtagccc
      421 agggtggcga aggtggtgtc ggctatgggc gcgtcggtgt tcacggcgat gcggatcagc
      481 acatggccca cggcctgcgg gtcaaagccg atggagcgca ccttgcccac ggccacgccc
      541 ttgtagcgca cggcggcctg gggctgcagg ccgctgacgc tttccttgct ggagagctcg
      601 tacacccggt agtcggtctg gtcgcgcgtg agccacatgg ccagacccgc cagcagcgcg
      661 gcgaccgcta tcacgaagat gcccgcggcc agggcatgtg ctttgttttc catcaggcct
      721 cctggggcgc agcggcgggg tcaggcggct gcgccatggc gcggcgcccg cgttcgccgg
      781 caaagtaatg gtgaatgaaa ggatggtcga actgcgccac ctgctgcgcc gtgccgctgg
      841 tgatgacatg ccggtcggcc agcacggcca cgcgcgtgga cagggcaaac aacgtgtcca
      901 ggtcatgcgt gaccatgatc accgtcaggc ccagcgcggc gtgcagctcg cgcagcagtt
      961 cgcagaattc gtccgagctg aacgggtcca gccccgccgt gggctcgtcc agcagcagca
     1021 gcggcgggtc catgatcagc gcgcgcgcca gggccacgcg cttgatcatg ccgcccgaga
     1081 ggtcggccgg catgcgcgtg gcgtgctcgg ccttcagccc cgccaggcgc agcttgacca
     1141 tggccacgtc ctgcaccagc gcgggcggca gcgtgcccag ctcgcgcagc gcaaaggcaa
     1201 tgttgtccag cacgttgaag gccgagtaca gcgcgccatg ctggaacagc atgcccacgc
     1261 gggcgctggc gccctcgcct ccgagctcgg cggcgggccg gcccagcacc gtgacgctgc
     1321 cgcgcgtggg ccgcgtcagg cccaggatat ggcgcagcag caccgtcttg ccggtgccgg
     1381 agccgcccac cagcgccagg atttcgccgc ggcgcacggt cagctgcagt tggtcatgca
     1441 cggtgaaggc gctgtcgccc tggccgaaga cggtggccaa gtcgcggatc tcgatgatgg
     1501 tgtctgcatc cgccatgatc agaatcccac gctgcggaag gccacggcga acaccgcgtc
     1561 cacgatgatg accatggtga tggcgctgac caccgaggcc gtcgtgccct cgcccaggct
     1621 ttgcgtgttg ggcttgacgc gcagccccca gtggcagccg atcagggcga tgaacacgcc
     1681 gaacaccacc gacttggcca tggccagcca gaggttggag acttgcaccg ccgccggtag
     1741 cacctgcagg aaataggccg ccgagatgcc catggtcaga tccgccgcca gcatgccgcc
     1801 ggccagcgcc gccagcgtgg tccacagcgc gatcagcggc atggccaggc ccagcgccag
     1861 cgcacgcggc agcaccaggc gaaagccatg cgctatgccc atgacgcgca tggcgtcgag
     1921 ttcctcggtc acgcgcatca cgccgatctg cgcggtgatg gccgagcccg agcgcccggc
     1981 caccaaaatg gcgcccagca taggcccgag ctcgcggatc agcgagatgc ccaggatgtt
     2041 gacgatgaag gcctcggcgc caaactggcg cagctgcagg ctcatcagat aggccagcac
     2101 cacgccgatc agaaaaccca ccagcgccgt gatcggcagg gcccgcgcgc ccatctggta
     2161 gagatggccc gagatgtcgc gccagggcgc gcgcaagggc ttcgccgcca ggcgcagcat
     2221 gtccagcagc agctggccca ccagcaacac caggcccgtg aggtggtcca gcgcgtgcag
     2281 caccagctcg cccaggcggt cgatctgcgt ggacaggcgc cagggggcgg gggccgcgca
     2341 gggtgtggag aactgcgcca cgcgctccag catctggcgc tgggtgtcgc tcagttcgat
     2401 ctgccccggc caggcgcggc cccagtggtt ccacaggatt tgcgcgccca catgatccag
     2461 ccgcgccaag tcgcgcaggt tccaggcatg gccggggccg caggccttga gctcgccggc
     2521 cacgcgccgc caggcctggc gctcggcaaa ggccgccgcc acccagtcac cctgcggcag
     2581 ggcgcagggc gtggcggtgc tgtcttgcac gatggtcgga ggggacggga cgggcatggt
     2641 ggtggtaacg gggccgcaga cgcgaacggg atgcggcgca tcatttcaca gattgacacg
     2701 ggcctgtggc gggccatgaa aaaacatcaa aatctggcca tgacgcgcat gggctgttcg
     2761 ccaaaagcaa tcaaaaccat agcatgaagg agttcccctc tcgacacttc agcgccgatc
     2821 cgcacaatcc gccgcagcac cgcgccatcc ctgcgcggca ttctctccga cgacggcccc
     2881 catgagcgac accactttcg actacatcat catcggcggc ggcacggccg gcgccctgct
     2941 cgccaaccgc ctgagcgccg acccccaggc ccgcgtgctg ctggtggagg ccgggcgcaa
     3001 ggacgactac cactggatcc acattcccgt gggctacctg tactgcatag gcaacccgcg
     3061 caccgactgg ctctacaaca ccgagcccga cgccggcctg aacggccgca gcctgcgcta
     3121 cccgcgcggc aagacgctgg gcggctgcag cagcatcaac ggcatgatct acatgcgcgg
     3181 ccaggcgcgc gactacgacc aatgggccga catcaccggc gacgacgcct ggcgctggag
     3241 caacgtgctg cccgccttca ggcgccacga ggaccattgg cgcctggacg agccgggcag
     3301 cgccagcgag gccttccagc agctgcacgg caacaagcgc accggcagca cgggcgaatg
     3361 gcgcgtggac aagcagcgcc tgcgctggga catcctggat gcatttgccc aggccgcgac
     3421 cgaggccggc attcccgcca ccgacgactt caaccgcggc accaatgagg gcgtgggcta
     3481 cttcgaggtg aaccagaagg acggctggcg ctggaacacc gccaaggcct ttctgcgccc
     3541 gacctgctac ggccggccca acttcgaact ctggacatcg gcccaggcca cgcgcgtgct
     3601 caccgaaaag caggccgacg gcagcctgcg ctgcaccggc gtgcaggtgt ggacgggcga
     3661 ggagatggtc accgcccacg cccagcagga ggtcatcctg agcgccggcg ccgtgaactc
     3721 gccgcagatc ctgcagctgt cgggcatggg cccggcggcg ctgctgcgcc agcatggcat
     3781 tgatgtgcgg ctggacctgc ccggcgtggg cgccaacctg caggaccacc tgcagatccg
     3841 cgccgtgtac aaggtgcaga acgtggccac cctgaacacc ctggccaata gcctggtggg
     3901 caaggcaaaa attggcctgg agtacctgct caagcgtagc gggccgatga gcatggcgcc
     3961 gtcgcagctg ggcgccttca cgcgcagccg gcctgatctg ccctggccca acatcgaata
     4021 ccacgtacag ccgttgtcgc tggaggcctt cggcgagccg ttgcacgact tcccggcctt
     4081 caccgccagc gtctgcaacc tgaacccgac cagccgcggc agcgtgacca tcaagaacgc
     4141 cgactttcgc agcgcgccgg cgatcgcgcc caactacctg tccacccccg aggatcgcca
     4201 ggtggcggcc gacagcctgc gcatcacgcg ccacatcgtc gcccagccgg cgctggcgcg
     4261 ctaccgcccc gaggaataca agcccggccc gcagtaccag agcgacgagg aactggcgcg
     4321 cctggccggc gacatcgcca ccaccatctt ccaccccgtg ggcacgacgc gcatggggcg
     4381 catggacgac cccatggccg tgctcgactc gcgcatgcgc ctgctcgacg gccgcggcgg
     4441 cgcggtcgcc ggcctgcgcg tggtggacgc gggcgccatg cccagcatca ccagcggcaa
     4501 caccaacgcg cccacgctga tgatggccga gatggcggcg cagtggatca tcgaggcctc
     4561 gcgcgccaag aggtgacccc gctgcagcca cgatggcggc ttgcgggaaa tcaccaatgt
     4621 ctcttgcgcg actgctgccg tacagtcttg ccactcgacg cgtgcctcgg ctcgccgcac
     4681 gaggggaccg aggagacaaa ccccgaaatc aacaacacgc acatcattga ggcatccacc
     4741 atgagatgcc atttctcaag gcacctgcaa cccaggtgcc ttttttattg cccggcgcgg
     4801 tagcctctgc gcacgtagga caatcgccgc catggaatgg atcatcgtct cgctggcctc
     4861 gctgctggcc ggttttgtag atgcgatcgt gggcggcggc ggcctgatcc tgctgcccgc
     4921 actgtttgcc acctttgcca gcgccccgcc cgccacgctg ctgggcacca acaaggcggc
     4981 ggccttctgg ggcacctcca tggccacctg gcagtacagc aaacgggtgc aaatcccgtg
     5041 gcgcgccatg ctgccgggcg tcgccgcggg gtttgtcggt tcattcatcg gcgcctgggc
     5101 cgtgacccag atgtcgcccg acttcctgcg caagctgctg cccctggtga tggtggcggt
     5161 gctggcctac accctggcca agaaggacct gggcctgcag catgccccgc gcttcagcgg
     5221 ccgcaccgag cgccttatcg tcggcaccat cggcctggtg atcggtctgt atgacggttt
     5281 cttcggcccc ggcaccggca gttttttcgt gttcgccttt gtgcgcctgc tgggctatga
     5341 cttcttgaac gcctcggtct cggccaagct gctgaacctg gccaccaatg tctcggccat
     5401 cattctgttt gccgcgaagg gccatatctg gtggcatttc atggtgccgc tggccgtggc
     5461 caacgtactc ggcagcatgc ttggcaccta catggccctg cgccacggca cgggctttgt
     5521 gcgcgccatc ttcatcctgg tggtgggggc gctgatattg aagaccggct acgacgcctt
     5581 tctgcgctga aggagaaatc acccatgacc gagctgaccc tgataggcgc acccaccgac
     5641 gtcggcgcca gcgtgctggg cgccagcatg gggcccgatg cgctgcgcat cgccggcata
     5701 gcgcgcgccc tgcagggcct gggccttgcg gtgcaggacc tgggcaattt gagcggaccg
     5761 ggtaacccac aggcggcgcc gcaaggtggg tttcgccatc tcgtcgaggt cacccgctgg
     5821 aacgaggcgg tgttcgccgc cacgctgtcc gcgctgcagg ccgggcggct gccgctgctg
     5881 atggggggcg accactgcct ggcgatcggc tccatcagcg ccgtggcgcg ccactgcagc
     5941 gcgcggggca ggcggctgaa ggtgctgtgg tttgacgccc atgccgacgc gaacacgccg
     6001 gcgtcctcac ccagcggcaa cctgcacggc atgccggtgg cctgcctgct cggccatggg
     6061 ccggcgcagc tgacgcagct ggccggcgcc gcagcggccc tgcaggcgca gcagatcacg
     6121 ctggttggcg tgcgcagcgt ggacgcgacg gagaaaagct tcgtcaacac cctgggcata
     6181 gaggtctacg acatgcgcag catcgacgag ctgggcatgc gcgcggtgat gcaggcggcg
     6241 ctggccgacg tggacgagaa cacccacctg cacctgagct tcgacatgga cggcctggac
     6301 cccaccatcg cccccggcgt gggcacgccg gtgcgcggcg gccccaccta ccgcgagaca
     6361 cagctgttca tggagatgct ggccgacagc ggcgccttag gctcggtgga catcatggag
     6421 ctgaacccgg cgctggatct gcgcaaccag accgccgagc tggcggtaga cctactcgag
     6481 agcctgttcg gcaagtccac gctgatgcgc gcctgaacca gccgggcatc ggccgcaaca
     6541 ggggtttgtt gcaatgtatg catggcctta tgcataccta taatgcagcc gcctcgccag
     6601 gccccgaccc gggtcgccag cgtggccaac gacttccggc ttgtggatca ccagccggat
     6661 tttttgtacc tgagagcgtg gtcacgctcc cataccacgc aggaaccgct tcatgaagat
     6721 gctcaagacc ctggccgccg ccctggccgt ttgcgccgcc gtcaccgtgc aggcccgccc
     6781 cctggaagac atccagaaga gcggcaccat catcctcgca tccgaaggcc agtacgcgcc
     6841 cttcaacttc ttccagggca agcagctgac cggctacgag atcgaggtcg gcgaggccgt
     6901 ggccaagaag atgggcctga agttcgagtg gaagacggtg ggctttgacg ccctgctgac
     6961 cggcctggcg caagaccgct gggatctggt catcgccagc cacgccatca ccgaagagcg
     7021 agccaaggcc gtgaccttca cggccccgca ttactgctcg ggcggccaga tcgtcagcat
     7081 gacgccggcc atcgccaagg ccgccgacct ggccggcaag accgtcgccg tgcagaccgg
     7141 cacgacctac ctggacgccg tgaagaaggt gcccggcgtc aaggaggtga agaacttccc
     7201 gaccgatgac gccgcccgca acgccctggg cagcaagcgc gtggacgcct gggtgaccga
     7261 ccgtttcgtc gccaaggaga tgatctccaa gctgcccaag gccggcttca agatcggcga
     7321 catgctgttc atcgagcagg tcgcctccgc cgtcaccaag ggcaaccagg gcctggccga
     7381 cgcctggaac aaggcgctca aggagctgat cgccgacggc accgtgggca agatcagcca
     7441 gaagtacttc caagaagacg tcacctgcaa ataacccgca gcgccgggcc tgcgcgcccg
     7501 gcccgccttg cccatgcttc tcgctctctg gcccgaccac tggagccgct cccagcgcag
     7561 caatgcgacg ctggtggcgg ctttggtttt gatggcgctg gtactgtccc tgctgggcca
     7621 gctgctgtcg ttctttcctg aacccatagg ctccaacgcc gcgctgtttg ccgatggcgc
     7681 gctgaccacg ctgtacctga ccctggtcag cggcgccgcc ggcctggtgc tgggcacggg
     7741 cgcggcgctg gcgcgcacct cgcgcctggc ctggctgcgc tatgtggcca gcttctacat
     7801 ctgggccata cgcggcacgc cgctgttggt gcagattctg ttcgtctact ttgccctgcc
     7861 ggtgctggtg ccggggctga acctgcccga ctttgccgcc gccgtggtgg cgctgggcct
     7921 gaacgtcggc gcctacaacg ccgaggccat tcgcgccggc ctgctggccg tgccacgcgg
     7981 ccagaccgag gcggcgcgcg cgctgggcct gggccacggc catgtgttcc tcgacgtggt
     8041 gttcccgcag gccttcaaga tcgccctgcc accgctggta agcaactttg tcgcgctgct
     8101 caaggactcg tcgctggcct acgccatcgg cgtggtcgag ctgaccaacg tcggcaaccg
     8161 catccagtcg gccaccttcc agccggtggc cacgctgacc acggtggcca tcacctatct
     8221 gatcctgacc acgctggtga cgcaggtgac gcacgcggtc gagtaccgct tcgacgtcga
     8281 ggggcgcgcc aaatgagcaa ggcctatatc gtctgccgcg acgtgcgcaa gcgctttggc
     8341 gagcacgagg tgctcaaggg cgtctcgacc gagttccaga ccggccaggt caccgtcatc
     8401 atcggcgcct cgggctcggg caagagcacg ctcttgcgcg ccatcaaccg gctggagccg
     8461 cacgacagcg gcagcatcac catcgacggt gtcgaggtca gcgacgacca gcgcacgctg
     8521 caaaagcagc gcagcgaggt cggcatggtg ttccagcagt tcaacctgtt tggccacctg
     8581 agcgtgctcg acaacgtgac gctggcgcca aggcgcatcc gccgcacgcc gcgcaccgcc
     8641 gccaacgagc aggccatggt gctgctcaag cgtgtgggca tgcaggacca tgcgcacaaa
     8701 tacccctggc agctatcggg cgggcagcag cagcgcgtgg cgattgcgcg cgcgctggcc
     8761 atgcagccca aagtgatgct gtttgacgag ccgaccagcg ccctcgaccc cgagatggtg
     8821 caggaggtgc tggacgtgat gcgcgagctg gcgcgcggcg gcatgaccat gatcgtggtg
     8881 acgcacga
//
LOCUS       DAOMTQ010000123         8862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1659_length_8862_cov_27.355626, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000123 DAOMTQ010000000
VERSION     DAOMTQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1659_length_8862_cov_27.355626"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PK518_11555"
     CDS             complement(<1..930)
                     /locus_tag="PK518_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamide-nucleotide
                     adenylyltransferase/Nudix hydroxylase"
                     /protein_id="HRM49595.1"
                     /translation="MYDAAVLIGRLQPLHQGHLALLHEALARAHRAIVVLGSAHQART
                     PRNPFTWQERAQMLRDALPQAERARLIVLPMRDYYDEPRWAQAVRQAVAENTPAAARI
                     ALVGHFKDDTSGYLRAFPGWELIRMARQGAIDATPIRDAYFGATPDSLDAALAPLAQH
                     LPTATRSWLQQFAYTPHYPALQEEWRMLRAYRASWAGAPYPPVFVTVDALLRCQGQVL
                     LIRRGHAPGKGLWALPGGFVEPNDTLWQSCLRELQEETHCTLPEAHLRTALRGQRIFD
                     HPQRSLRGRVITQAYFFDLGQEPLPEVRGGDDAA"
     gene            complement(1135..2316)
                     /gene="metK"
                     /locus_tag="PK518_11560"
     CDS             complement(1135..2316)
                     /gene="metK"
                     /locus_tag="PK518_11560"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRM49596.1"
                     /translation="MANDFLFTSESVSEGHPDKVADQISDAILDAALAQDPLSRVAAE
                     TLTNTGLVVLAGEITTNAHVDYIQVARDTIQRIGYDNTEYGIDYKGCAVLVAYDKQSN
                     DIAQGVDHASDDHLNTGAGDQGLMFGYACDETPELMPAPIHYAHRLVERQAQLRKDGR
                     LPFLRPDAKSQVTMRYVDGKPHSIDTVVLSTQHSPDQSENAHKMKGSFIEAIIEEIIK
                     PVLPAEWLKDTKYLINPTGRFVVGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDP
                     TKVDRSAAYAARYVAKNVVAAGLARQCQVQVAYAIGVARPMNITVYTEGTGVIPDEKI
                     AAIVAREFDLRPKGIIQMLDLLRPIYAKTAAYGHFGREEPEFTWERTDKAAALRAAAG
                     L"
     gene            2452..3318
                     /locus_tag="PK518_11565"
     CDS             2452..3318
                     /locus_tag="PK518_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM49597.1"
                     /translation="MPSVFRLFSVLPLWLLHAIGAVLGWVAFLASPTYRRRFLDNAAC
                     AGYALADVRAAVAHAGRMVAEAPRLWLAPESPACEIRNPECVERAWSRGRGIVFLTPH
                     IGCFELSVQAVSQRWSPVHGPITILYRPARQRWLAEVMQTARNRPGIAAVPTTLQGVR
                     QMLKALRRGEAVGLLPDQVPPQGLGLWSPFFGREAYTMTLAARLVQQTGASVILARCE
                     RLTFGRGYVLHLQDLAAPLAPELEAAVLQINQAMEQLIRQSPEQYLWGYARYKQPRAE
                     MHGSRDAAEGGA"
     gene            3315..4187
                     /locus_tag="PK518_11570"
     CDS             3315..4187
                     /locus_tag="PK518_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HRM49598.1"
                     /translation="MKTRLAVALMGLLAHLPLPLLRGLGWLLGRLLFVLAAPRRKVAL
                     RNLALCFPEVPEAQRRAWARATFVVFCQTFLDRSWLWAGSEALVRRRVKLVGAVHELD
                     GDTPTIVFAPHFYSMDAGGLALPLNTAREFTSIFATNPDPALDAWFMGGRQRFGNVRM
                     LNRADGVKPIISCLRKGGLLYLLPDMDYGPGDSVFVPFFAMPDAATIPSLSRFARLGR
                     AKVVALYSRMTPDGYVAELTPAWENFPTEDHVQDTARMNRELEAAIRTMPAQYYWVHK
                     RFKTRPEGQASVYG"
     gene            complement(4263..4940)
                     /gene="yihA"
                     /locus_tag="PK518_11575"
     CDS             complement(4263..4940)
                     /gene="yihA"
                     /locus_tag="PK518_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRM49599.1"
                     /translation="MSTTPQAPAPGALPDAKVAMGWMHTARFLTTAAQLHQLPAIEVP
                     EIAFVGRSNAGKSTCINTLAQQRQLAFASKKPGRTQHINLFALGKQGVMDAVLADLPG
                     YGYAAVSRSDKQRWQQVMANYLVSRKSLSGIVLLCDPRLGLTELDEALLEVVRPRVEQ
                     GLKFLVLLTKADKLTRSEQAKALSITRLNAGGGEVRMFSALKKQGVDEVAQLLWQWAH
                     PAVDGES"
     gene            5023..5655
                     /locus_tag="PK518_11580"
     CDS             5023..5655
                     /locus_tag="PK518_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRM49600.1"
                     /translation="MKLLASLLMAAALAAPAIPAFAQGSAPAQPAKPDLAKGEASYAA
                     VCAACHAADGNSTIAVNPMLAQQHPEYLVKQLKEFKSGKRADPVMQGMAAMLSDDDMR
                     NVAAWLASQKAKEGFAKDKDLVSLGERIYRGGIQDRHIAACAGCHSPNGAGIPAQYPR
                     LSGQHGDYTVKQLTDFRDGKRGNNNQMRDVAAKLNDREIKAVADYIAGLR"
     gene            5785..7965
                     /locus_tag="PK518_11585"
     CDS             5785..7965
                     /locus_tag="PK518_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRM49601.1"
                     /translation="MSDHSLGHGRAPRSPATRSITELLSSMRFAIALLTVICIASVIG
                     TVMKQHEPVNNYVNQFGPFWADLFLALKLNAVYSAWWFLLILAFLVISTSLCIARHTP
                     KYLADLRNYKENIRAQSLQAFHHKAGATLAGEDTGAAAQRIGKLLVSGGWKVRLQQRQ
                     TPEGDGWMVAAKAGAANKLGYIAAHSAIVLVCLGGLLDGDLIVRAQMWFGGKTPYPGG
                     GFIADVKPEHRLPATNPTFRGNLLVTEGRHSSTAILSQSDGMLLQELPFSIELKKFVV
                     EHYSTGMPKLFASDIVIHDLATGEAKAARVEVNHPVRHRGVEIYQSSFDDGGSELKLR
                     ARPLVPGAEPFDVAGVVGGSTQLTNTGTGEALTLEFTGLRVLNVENFGDNGASASGAD
                     VRKVDLRESIESRLGAGNKTVAKRELRNVGPSVSYKLRDAAGQAREFNNFMLPVDTGD
                     GQAVFLLGVRETQADPMRYLRVPVDAEGRMDGFVRLRLALADPALREKAVRRYAAAAV
                     DAKRPELAEQLAQSAARALALFAGDTLGDGTMEKLAAVNGRPLAGLPAISEFMEANVP
                     EGERERAAEVLMRIMNGALFELAQITREQAGLAPLEPGEKTQAFMTQAVLSLSDSQAY
                     PAPMVFELTDFKQIQASVFEVARAPGKNVVYLGCALLIIGIFAMLYVRDRRLWVWLAP
                     GSGGAQATMALSANRKTLDTDREFAQLRTQLLGTDETLPHGGKT"
     gene            7962..>8862
                     /locus_tag="PK518_11590"
     CDS             7962..>8862
                     /locus_tag="PK518_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HRM49602.1"
                     /translation="MNTATTTLTLNEGYFARRNWLDWLFAALVLAGGLFALQRYGAHM
                     DVYEKGILLGSIPGAIWLGWFWRPLRLLMLAVAGCSLLAIGLYQNNGAGDLARAETVF
                     GLKYFLSSQSAILWMSMLFFMSTAFYWIGMFSRGQGPAMMRIGSRIAWVAVALALIGT
                     MVRWYESYQIGPDIGHIPVSNLYEVFVMFCWMTAVFYLYYEEQYATRALGGFVMLVVS
                     AAVGFLLWYTVVREAHEIQPLVPALNSWWMKLHVPANFIGYGTFALSAMVAFAYLIKQ
                     QAQETRWYKLAPLWLLGVVLCFEP"
BASE COUNT         1339 a         2896 c         3039 g         1588 t
ORIGIN      
        1 cgccgcgtcg tcgccgccgc gcacctcggg caggggttct tgccccaggt cgaagaagta
       61 cgcctgggtg atgacgcgcc cgcgcaggct gcgctgcgga tgatcgaaga tccgctggcc
      121 gcgtagtgcc gtgcgcaggt gcgcttcggg cagggtgcag tgggtttcct cttgcaattc
      181 acgcaggcag gactgccaga gcgtgtcgtt cggctcgaca aagccgccgg gcagcgccca
      241 cagccccttg ccgggggcgt ggccgcggcg gatcagcagt acctggccct ggcagcgcag
      301 cagcgcgtcc acggtgacga agaccggcgg gtagggcgcg ccggcccagc tggcgcggta
      361 ggcgcgcagc atgcgccatt cctcctgcag ggcggggtag tgcggggtgt atgcaaattg
      421 ctgcagccag ctgcgcgtgg cagtgggcag atgctgggcc aggggggcga gcgcggcatc
      481 cagcgagtcc ggcgtggcgc caaagtaggc gtcgcgtatc ggggtggcgt cgatggcacc
      541 ctggcgcgcc atgcggatca gctcccagcc ggggaaggcg cgcaggtagc cgctggtgtc
      601 gtccttgaaa tgaccgacca gggcgatgcg cgctgctgcc ggcgtgtttt cggcaacggc
      661 ctggcgcacc gcctgcgccc agcgcggctc gtcgtagtag tcgcgcatgg gcagcacgat
      721 gaggcgcgcg cgctctgctt gtggtagcgc atcgcgcagc atctgtgcgc gctcctgcca
      781 ggtgaatggg ttcctcggtg tgcgcgcctg gtgcgccgag ccgagcacga cgatggcccg
      841 gtgggcacgc gccagcgcct cgtgcagcag ggccaggtgg ccctggtgca gcggctggag
      901 gcggcctatg agaacggcgg cgtcgtacat ggtttcaaat gctccttgaa taatagcgtt
      961 cagcgcttat tcatcgggcg gtagggccga attttctcaa aaaccgtcag tgggttgcat
     1021 gggcttacag ccccgccgcg gcgcgcagcg cgaccgcctt gcggtctgcg ttcacagcgc
     1081 tgcgcgctat gggctgcgcc cgcgtaccgt cactgcgccg aaggcgcagc gggcttacag
     1141 cccggctgcg gcacgcagtg ccgcagcttt gtcggtacgc tcccaggtga attcgggctc
     1201 ttcgcggccg aagtggccgt aggcggcggt cttggcgtag atggggcgca gcagatccag
     1261 catctggatg atgcccttgg ggcgcaggtc gaattcgcgg gcgacgatgg cggcgatctt
     1321 ctcgtccggg atgacgcccg tgccctcggt gtagacggtg atgttcatcg gccgcgccac
     1381 gccaatggca taggccacct gcacctggca ttggcgcgca agaccggcgg cgacgacgtt
     1441 cttggcgaca tagcgcgcgg catacgctgc gctgcgatcc accttggtcg ggtccttgcc
     1501 gctgaaggct ccgccgccat gcgggcaggc gccgccgtag gtgtccacga tgatcttgcg
     1561 cccggtcagg ccgcagtcgc cctgcggccc gccgacgacg aagcggccgg tggggttgat
     1621 caaatacttg gtgtccttga gccactcggc cggcagcacg ggcttgatga tctcttcgat
     1681 gatggcctcg atgaagctgc ccttcatctt gtgcgcgttc tcgctctggt ctggactgtg
     1741 ctgggtggac agcaccacgg tgtcgatgct gtgcggcttg ccgtccacat agcgcatggt
     1801 cacctggctc ttggcgtcgg ggcgcaggaa gggcaggcgg ccgtccttgc gcagctgcgc
     1861 ctggcgctcg accaggcggt gcgcgtagtg gatgggcgcg ggcatcagct cgggcgtctc
     1921 gtcgcaggcg tagccaaaca tcaggccctg gtcaccggcg ccggtgttca ggtggtcgtc
     1981 cgacgcgtga tccacgccct gggcgatgtc gttgctctgc ttgtcgtagg ccaccagcac
     2041 ggcgcagccc ttgtagtcga tgccgtactc ggtgttgtcg tagccgatgc gctggatggt
     2101 gtcgcgcgcc acctggatgt agtccacatg ggcgttggtg gtgatctcgc cggccagaac
     2161 caccaggccg gtgttggtca gcgtctcggc ggccacgcgc gagagcgggt cttgcgccag
     2221 ggcggcgtcg aggatggcat ccgagatctg atccgccacc ttgtcggggt ggccttcaga
     2281 gaccgattcg gaagtgaaga ggaaatcgtt cgccatttga ataaactcca gtatttgcag
     2341 catttgggcg ttgcccacag ctttcaggag ccttgtggcg aacgctttag cagttgtgtt
     2401 taacgtcgcc ctgcaagttg ctcatttaac tcggcgacac ccgccattct aatgcctagc
     2461 gtttttcgcc ttttttctgt gttgccgttg tggttgctcc acgccattgg ggcggttctg
     2521 ggctgggtgg cgtttctggc atcgcccacc tatcggcgcc gttttctgga caacgccgcg
     2581 tgcgccggct acgcgctggc cgatgtgcgc gccgccgtgg cccatgccgg gcgcatggtg
     2641 gccgaggcgc cgcgcctgtg gctggcgccc gagtctcccg cctgcgagat ccgcaacccc
     2701 gaatgcgtcg aacgcgcctg gagccggggc cgcggcatcg tgttcctgac gccgcatatt
     2761 ggctgcttcg agttgtcggt gcaggccgtc tcgcagcgct ggtcgccggt gcatgggccg
     2821 atcaccatcc tgtaccgccc ggcgcgccag cgctggctgg ccgaggtgat gcaaaccgcg
     2881 cgcaaccgcc caggcatagc ggcggtgccg accacgctgc agggcgtgcg ccagatgctc
     2941 aaggcgctgc gccgcggcga ggccgtgggc ctgctgcccg accaggtgcc gccccagggc
     3001 ctgggcctgt ggtcgccgtt ctttggccgc gaggcctaca ccatgaccct ggccgcgcgc
     3061 ctggtgcagc agaccggcgc cagcgtgatc ctggcgcgct gcgagcgtct gacgttcggg
     3121 cgtggctatg tgctgcacct gcaggatctg gccgcgccgc tggcgccaga gctcgaagcg
     3181 gccgtgctgc agatcaacca ggccatggaa cagctcattc gccagagccc cgagcaatac
     3241 ctgtggggct atgcgcgcta caagcagccg cgtgcagaga tgcatggcag ccgtgacgct
     3301 gccgagggcg gcgcatgaag acgcgcctgg ccgtggccct gatgggcctg ctggcgcacc
     3361 tgcccctgcc gctgctgcgc ggcctgggct ggctgctggg ccggctgctg ttcgtgctgg
     3421 ccgcgccacg gcgcaaggtg gcgctgcgca atcttgcgct gtgctttccc gaggtgcccg
     3481 aggcccagcg ccgcgcctgg gcgcgcgcga ccttcgtggt gttctgccag acctttctgg
     3541 atcgcagctg gctctgggcc ggctccgagg ccctggtgcg ccgccgtgtg aagctggtgg
     3601 gcgccgtgca tgagctcgat ggcgacacgc ccaccatcgt tttcgccccg catttctaca
     3661 gcatggacgc gggtggcctg gcgctgccgc tgaacacggc gcgcgagttc acctccatct
     3721 ttgccaccaa ccccgacccg gcgctggacg cctggttcat gggtggacgc cagcgctttg
     3781 gcaatgtgcg catgctcaac cgcgcggacg gcgtcaagcc catcatctcc tgcctgcgca
     3841 agggcgggct tttgtacctg ctgccggaca tggactatgg gccgggcgac tcggtcttcg
     3901 tgcccttctt cgccatgccc gatgccgcca ccatcccctc gctgtcacgc tttgcgcgcc
     3961 tgggccgggc caaggtggtg gccttgtaca gccgcatgac gcccgatggc tacgtggccg
     4021 agctgacgcc cgcctgggag aacttcccca ccgaggacca tgtgcaggac accgcgcgca
     4081 tgaaccgtga gctggaggcg gccatccgca ccatgcccgc gcagtactac tgggtgcaca
     4141 agcggttcaa gaccaggccc gagggccagg cttcggtcta tgggtgaatg gttctgtggt
     4201 gagtagtaaa cggtaaatgg tgagtggtga atggttacga ctttccattc accacttacc
     4261 acttacgact caccatcaac cgccggatgc gcccactgcc acagcagctg cgccacctcg
     4321 tccacgccct gttttttcag cgccgagaac atgcgcacct cgccgccgcc ggcgttcagg
     4381 cgggtgatgg acagggcctt ggcctgctcc gagcgtgtga gcttgtcggc cttggtcagc
     4441 agcaccagga acttgagccc ctgctcgaca cgcgggcgca ccacctccag cagcgcctcg
     4501 tcgagttcgg tcaaccccag gcgcgggtcg cacagcagca cgatgcccga caggctcttg
     4561 cggctgacca ggtagttggc catcacctgt tgccagcgct gcttgtcgct gcgcgagacg
     4621 gcggcgtagc cgtagccggg caggtcggcc agcaccgcgt ccatcacgcc ctgcttgccc
     4681 agcgcgaaca ggttgatgtg ctgcgtgcgc cccggcttct tcgaggcaaa ggccagctgg
     4741 cgctgctgcg ccagggtgtt gatgcaggtg gacttgccgg cgttggagcg gccgacaaag
     4801 gcgatctcgg gtacctcgat ggcgggcagc tggtgcagct gcgcggcggt ggtcagaaag
     4861 cgtgcggtgt gcatccatcc catggccacc ttggcgtcgg gcagggcgcc gggcgcgggt
     4921 gcttgtggcg tggttgacat agattggcct caagggctgg aacaaatcag ggtgcattgt
     4981 agaatcgccg ggttttgcgc tcataagaca agaaccctcg atatgaagtt gctcgcctct
     5041 ttgctcatgg ccgccgccct ggctgccccc gccattccag cttttgcgca gggctcagcc
     5101 ccggcgcagc ccgccaagcc tgacctggcc aagggcgagg ccagctacgc cgcggtgtgc
     5161 gccgcctgcc acgcggccga tggcaactcc accatcgccg tcaaccccat gctggcgcag
     5221 cagcaccccg aatacctggt caagcagctc aaggaattca agtccggcaa gcgcgccgac
     5281 ccggtgatgc agggcatggc cgccatgctg agcgacgacg acatgcgcaa cgtggccgcc
     5341 tggctggcct cgcaaaaggc caaggaaggc tttgccaagg acaaggacct ggtctccctg
     5401 ggcgagcgca tctaccgtgg cggcatccag gatcgccaca tcgccgcctg cgccggctgc
     5461 cacagcccca atggcgccgg cattccggcg cagtacccgc gcctgtcggg ccagcatggc
     5521 gactacaccg tcaagcagct gaccgacttc cgcgacggca agcgcggcaa caacaaccag
     5581 atgcgcgacg tggccgccaa gctcaacgac cgcgagatca aggccgtggc cgactacatc
     5641 gccggtctgc gttgattcac atcaattctc tgggggtgat tcgcggcatt cagggaacct
     5701 tgcgccgata atcgcaccca agggcgggcg ggcaaatctg aacggatggc ccgccttttt
     5761 cacattcggg ctcccgcgtt tctcatgtct gaccactctt tgggccacgg ccgcgcgccg
     5821 cgctcaccgg ccacgcgctc catcaccgag ctgctgtcgt cgatgcgctt tgcgattgcg
     5881 ctgctgacgg tgatctgcat cgcctcggtg atcggcaccg tgatgaagca gcatgagccg
     5941 gtgaataact acgtgaacca gttcgggccg ttctgggccg acctgtttct ggccctcaag
     6001 ctcaatgccg tctacagcgc ctggtggttt ttgctgatcc tggcctttct ggtgatcagt
     6061 acctcgctgt gcattgcgcg ccacacaccc aagtacctgg ccgacctgcg caactacaag
     6121 gaaaacatcc gcgcgcaaag cctgcaggcc tttcaccaca aggccggcgc cacgctggcg
     6181 ggcgaggaca ccggggccgc cgcccagcgc atcggcaagc tgctggtcag cggcggctgg
     6241 aaggtgcgcc tgcagcagcg ccagacgccc gagggcgatg gctggatggt cgccgccaag
     6301 gccggcgcgg cgaacaagct gggctatatc gcggcgcaca gcgccatcgt gctggtgtgc
     6361 ctgggtgggc tgctggatgg cgacctgatc gtgcgcgcgc agatgtggtt tggtggcaag
     6421 acgccttacc cgggcggcgg ctttatcgcc gacgtgaagc ccgagcaccg cctgcccgcc
     6481 accaacccga cctttcgcgg caacctgctg gtcaccgagg gccggcattc cagcaccgcc
     6541 atcctgagcc agtccgacgg catgctgctg caggagctgc cgttttccat cgagctgaag
     6601 aagttcgtcg tcgagcatta ctccaccggc atgcccaagc tgttcgccag cgacatcgtg
     6661 atccacgacc tggcgacggg cgaggccaag gcggcgcgcg tggaggtgaa ccacccggtg
     6721 cgccatcgcg gcgtggagat ctaccagtcg agctttgacg atggcggctc cgagctcaag
     6781 ctgcgcgccc gtccgctggt gccgggggcc gagccgtttg acgtggcggg cgtggtcggc
     6841 ggctccaccc agctcaccaa caccggcacg ggcgaggcgc tgacgctgga gttcaccggc
     6901 ctgcgcgtgc tcaacgtaga aaactttggc gacaacggcg ccagcgcctc gggggccgat
     6961 gtgcgcaagg tggacttgcg cgagtccata gagtcgcgcc tgggcgcggg caacaagacg
     7021 gtagccaagc gcgagttgcg caacgtgggc ccgagcgtga gctacaagct gcgcgacgcc
     7081 gccggccagg cgcgcgagtt caacaatttc atgctgcccg tggacaccgg cgacggccag
     7141 gccgtgtttc tgctgggcgt gcgcgaaacc caggccgacc ccatgcgcta tttgcgcgtg
     7201 ccggtggacg ccgagggccg catggatggc tttgtgcgcc tgcgcctggc gctggccgac
     7261 ccggcgctgc gcgagaaggc ggtgcgccgc tacgcggccg cggccgtgga tgccaagcgc
     7321 cccgagctgg ccgagcagct ggcacagtcc gccgcccgtg cgctggcgct gtttgccggc
     7381 gacaccctgg gcgacggaac catggagaag ttggccgcag tcaacggcag gccgcttgcc
     7441 ggcctgccgg cgatctcgga attcatggag gccaacgtgc ccgagggcga gcgcgagcgc
     7501 gccgccgagg tgctgatgcg catcatgaac ggcgcgctgt tcgagctggc gcagatcacg
     7561 cgcgagcagg ccgggctggc gccgctggag ccgggtgaga agacccaggc cttcatgacc
     7621 caggccgtgc ttagcttgag cgactcccag gcctatccgg cgcccatggt gttcgagctc
     7681 accgatttca agcagatcca ggccagcgtc ttcgaggtgg cgcgagcccc tggcaagaac
     7741 gtggtgtatc tgggctgcgc actgctgatc atcggcatct ttgccatgct ctatgtgcgt
     7801 gaccgccgcc tgtgggtctg gctggcgccg ggcagcggcg gcgcgcaggc gacgatggcg
     7861 ctgtcggcca accgcaagac gctggacacg gatcgcgaat tcgcgcagtt gcgcacccag
     7921 ttgctgggca ccgacgaaac ccttccccac ggaggcaaga catgaacacc gccacgacga
     7981 cgctgaccct gaacgaaggc tacttcgcgc gccgcaactg gctggactgg ctgtttgccg
     8041 cgttggtgct ggcaggcggc ctgtttgcgc tgcagcgcta cggcgcccac atggatgtgt
     8101 atgaaaaggg catcctgctc ggctccatcc ccggcgccat ctggctgggc tggttctggc
     8161 gcccgttgcg cctgctgatg ctggccgtgg ccggctgctc gctgctggcc atagggctgt
     8221 accagaacaa tggcgcgggc gacctggcgc gcgccgagac ggtgttcggc ctcaaatatt
     8281 tcctctccag ccagtccgcc atcctgtgga tgagcatgct gttcttcatg agcacggcct
     8341 tctactggat aggcatgttc tcgcgcggcc agggcccggc catgatgcgc ataggttcgc
     8401 gcatcgcctg ggtggcggtg gcgctggcgt tgataggcac catggtgcgc tggtacgaga
     8461 gctatcagat cggccccgac atcggccata tcccggtgag caacctctac gaggtgttcg
     8521 tcatgttctg ctggatgacg gccgtgttct acctgtacta cgaggagcag tacgccacgc
     8581 gtgcgctcgg cggcttcgtg atgctggtgg tcagcgccgc cgtgggcttt ctgctctggt
     8641 acacggtggt gcgcgaggcg cacgagatcc agcccctggt gccggcgctc aacagctggt
     8701 ggatgaagct gcatgtgccg gccaacttca tcggctatgg caccttcgcg ctgtcggcca
     8761 tggtggcctt tgcctacctg atcaagcagc aggcgcagga gacgcgctgg tacaagctgg
     8821 cgccgctgtg gctgctgggc gtggtgctgt gcttcgagcc ca
//
LOCUS       DAOMTQ010000124         8827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1666_length_8827_cov_25.651961, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000124 DAOMTQ010000000
VERSION     DAOMTQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_8827_cov_25.651961"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK518_11595"
     CDS             <1..230
                     /locus_tag="PK518_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HRM49603.1"
                     /translation="DTKHIKTQVLVENGGTVVIGGIFEMEETNQENKIPLLGDVPVVG
                     NLFKSRTKESMKREMLVFITPKVITDRGPVR"
     gene            251..1630
                     /locus_tag="PK518_11600"
     CDS             251..1630
                     /locus_tag="PK518_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49604.1"
                     /translation="MKILKTIFTSAALAGALVACGGGGGSSGSQPGKTFQTTAPSSVT
                     LLPGSSGQYDILGGVPPYRVGNSDTAIAVGAVSGTRLYIGAVNAGSSLVNVVDNSGAT
                     VGVTVNVGTSTPLTSTAPDALTIGVGAASMRTFTIRGGVPPYAVESSNANTFTATKIG
                     TNQFSVTGVAIGSGVVTVTDATNTKLGVNVTVGAPELRVSPTDLKIFPGIDAVVKISG
                     GQPPYKVAGGIPSAIQADISGDEMHIKGLLASKLELGVADATGQTVKVTVEVTNGTGV
                     FNIMPGSLSITEDDNQDIRLNIYGAAPGPVCFFTDRTQLQPQVGGCPSNPSIVTLVTG
                     SAGSRCVSGNTNVTLTAVDSNGAIANAIVTIVDNGKCAAQALTASANSVTVRAKTATI
                     DATSAQVLIYGGSGTYAITSTNSGVASAAIAGNVLTIKGGDYTGTASVTVYDQQQPTF
                     SVTITVTAN"
     gene            complement(1827..2672)
                     /gene="panC"
                     /locus_tag="PK518_11605"
     CDS             complement(1827..2672)
                     /gene="panC"
                     /locus_tag="PK518_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRM49605.1"
                     /translation="MQIARTLPELRAALAGRQRPAFVPTMGNLHDGHIALVRQARPLA
                     DTLVASIFVNRLQFLPHEDFDSYPRTWDADCARLEAADCDIVFAPRERDLYPEPQTFK
                     IQPDPALADILEGQFRPGFFTGVCTVVMKLFAAVLLPCGGGTAVFGKKDYQQLMVIRR
                     MVEQFALPVDIVAGDTSRASDGLALSSRNGYLDAGQRTQAVQLSQALQALAQAALAGS
                     APLPQLEERAMQALQAQGWAPDYLTVRQRHDLQPPQSGTATGTLVTLGAARLGGTRLI
                     DNLEF"
     gene            complement(2689..3570)
                     /gene="panB"
                     /locus_tag="PK518_11610"
     CDS             complement(2689..3570)
                     /gene="panB"
                     /locus_tag="PK518_11610"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRM49606.1"
                     /translation="MSQTTTATGTPYGTLPPASPLPQRRPISLPRLAQMREAGEKIAM
                     LTAYDATFAAVADAAGVECILVGDSLGMVCQGLPSTVGVQLDTMAYHTESVTRGLHRV
                     QGTAWVVADLPFGSYHESREQALRSASRLMQAGAHMVKLEGGGWTAPTVQFLVERGIP
                     VCAHLGLTPQTVHALGGYRVQGKGDAAAAELRRHALELQDAGAAMLVLEMVPAQLSTS
                     LTAELPHCHTIGIGAGKGTAGQVLVMHDMLGVNLGKNPKFVRDFMQGAASVRGAIEAY
                     VQAVKNGSFPDDSLHAW"
     gene            3870..4718
                     /gene="nadC"
                     /locus_tag="PK518_11615"
     CDS             3870..4718
                     /gene="nadC"
                     /locus_tag="PK518_11615"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRM49607.1"
                     /translation="MTPESIAALARADVARALQEDLGSGDLTAALVPEGRRCRARILA
                     REAAVICGAPWAEAALRALDPTVSLTWLVAEGQRCAPDQVVLDIAGDARALLSAERTA
                     LNFLQLLSAVATKTAIYVEAVKGTRAAIVDTRKTIPGLRLAQKYAVRVGGGVNHRIGL
                     HDAVLIKENHIAAAGGVTAVLRAAEAVAARAAFIEIEVETLAQLAEALDAGAKMVLLD
                     NMDLPTLRQAVEINAGRAILEISGGVTQDGLRALAETGVDRISIGALTKDVKATDFSM
                     RLQELA"
     gene            4715..5830
                     /gene="nadA"
                     /locus_tag="PK518_11620"
     CDS             4715..5830
                     /gene="nadA"
                     /locus_tag="PK518_11620"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRM49608.1"
                     /translation="MNTTTLKEVEYEQPEGGAVCSTKYAWARVPPEPAPDERERLKAR
                     IRRLLKEKNAVMVSHYYVHPDLQDLAEETGGIVSDSLEMARFGRDHAAQTLVVSGVRF
                     MGETAKILSPEKTVLMPDLDATCSLDLGCPIDEFSAFCDQHPDRTVVVYANTSAAVKA
                     RSDWLVTSSCALDIVRALKDKGHKILWAPDRHLGDYIRRETGADMLFWNGACIVHDEF
                     KALELELLIKEHPQAKVLVHPESPAEVIALADAVGSTSAILKAASEMDATEFIVATDK
                     GMMHKLRQLNPGKQFYEAPTAGNSATCKSCAHCPWMAMNGLADVARVLETGANAVHVD
                     PAIIPRARMPIDRMLAFTAALKNGQPAGALVPHFGAA"
     gene            6064..6375
                     /locus_tag="PK518_11625"
     CDS             6064..6375
                     /locus_tag="PK518_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HRM49609.1"
                     /translation="MRNRSALLTAALLGLAGSALAQSGDSTVNRSAQVASPGLSRAEV
                     LADLELWQRAGMTYWPHPPAEMDMQFTAEYQAGLARYRQLRGGQAMQQTAARYPDMPG
                     K"
     gene            6511..7665
                     /gene="pabB"
                     /locus_tag="PK518_11630"
     CDS             6511..7665
                     /gene="pabB"
                     /locus_tag="PK518_11630"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRM49610.1"
                     /translation="MNSIIDFPDPHDTAAARLCHAFGQPLRTLAAWGPGEVRGLLDAV
                     QQAAQAGLWCLGWLRYEAAAAFDTAYADALHATPEGRPLAWFAVHEPPAGAAQSSFAA
                     QAGEAPRVFWQPGLERAAFDAAIARIHAAIAAGDCYQINYTAQLAGRLLGDAQALFAA
                     LRRAQPGGYVACLDDGVEQVLSVSPELFFDWDGERILTRPMKGTAARGATPAQDAALA
                     QAMRASPKERAENVMIVDLLRNDLSRIAVAHSVRVPRLFHAEQLPTVWQMTSDVEART
                     RPGIGLADVFAALFPCGSITGAPKRQAMRLIRELEPGPRGIYCGAVGVVRPGSAPGRV
                     HATFNVPIRTVVVRGDELRCGIGSGITADARAEAEWQEWAAKRAFLRGLG"
     gene            7662..8285
                     /locus_tag="PK518_11635"
     CDS             7662..8285
                     /locus_tag="PK518_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRM49611.1"
                     /translation="MTDFQILETLALEQGVARHLDRHLARMAASSAHFGYPWDEARVR
                     ACLADLAAGHASGLWRVRLLLDGAGNPQAQAFALQPTAQPVRLQLADRPLQEAHDEWV
                     RHKTTRRAHYAAFAPAPGLFDTVLYNAAGEVTESTFGNIAVLLDGRWLTPALSCGLLP
                     GVGRAVALEQGRVEEAVLRLADLPRVQAWAFINSLRGWLEARLAAPG"
     gene            complement(8507..>8827)
                     /locus_tag="PK518_11640"
     CDS             complement(8507..>8827)
                     /locus_tag="PK518_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HRM49612.1"
                     /translation="AYPEAQALAELNAAVCASPEVLDEADLKSRFVRNASFHVGTVAS
                     GRGFVHAQLRLLSGRSPEAKQDLAARVAEVLRRLTPRPAGVMVQLSVEIVDMDRPSYV
                     KERL"
BASE COUNT         1451 a         2804 c         3043 g         1529 t
ORIGIN      
        1 tagacaccaa gcacatcaag acccaggtgc tggtggaaaa cggcggcacc gtggtcattg
       61 gcggcatctt tgaaatggaa gaaaccaatc aggaaaacaa gatccccctg ctgggcgatg
      121 tgcctgtggt gggcaatctg ttcaagagcc gcaccaagga atccatgaaa cgggaaatgc
      181 tggtgtttat tacgcccaag gtcattacgg atcgcgggcc tgtgcgttga ccatcgaaag
      241 tatgaaagtc atgaaaatat tgaaaacgat atttacctcg gcagcgcttg cgggcgcttt
      301 ggttgcctgc ggtggtggag gaggctcctc gggtagccag ccagggaaga cgtttcagac
      361 aacggcgcct tcgtcggtaa ccttgttgcc tggctctagt ggtcaatacg acatcttggg
      421 tggagtgccg ccgtaccgcg tgggtaatag cgatacggca attgctgtcg gcgctgtcag
      481 tggtacgagg ctgtacatcg gcgcagtgaa tgcgggtagt tctctggtca acgtggtcga
      541 caattcgggg gctacggttg gcgtaacggt gaacgtaggc accagcactc cgctgaccag
      601 caccgcgcct gatgcgctca cgattggagt cggggcggca tcgatgcgca cgttcacgat
      661 ccgcggcggt gttcctccct atgcggtgga aagctccaac gccaacacct tcaccgccac
      721 caagattggc acgaatcagt tcagcgtgac tggcgtggcg attggctccg gagtggtcac
      781 ggtgaccgac gcaaccaaca ccaagctagg tgtaaacgta acggtgggag cgccagagtt
      841 gcgggtgtct cccaccgatc tgaaaatttt ccctggcatt gatgcggtgg tgaaaatctc
      901 aggcggtcaa ccaccctaca aagtggctgg tggcattcca tcggccatcc aggctgacat
      961 ctcgggtgat gagatgcata tcaaaggcct gttggcatcc aagctggagc ttggggttgc
     1021 cgatgccacg ggacagactg ttaaggtaac tgttgaggtg actaatggca ccggcgtgtt
     1081 caacatcatg cctggctccc tttcgattac cgaagatgac aatcaggata ttcgtctgaa
     1141 catctatggg gcagcccctg gcccggtttg cttcttcacc gatcgcactc agttgcagcc
     1201 tcaagttggc ggttgcccga gcaacccgtc catcgtaacc ttggtgaccg gttctgcagg
     1261 ctcgcgttgt gtgagcggca atacaaacgt gactttgacg gccgtagaca gcaacggagc
     1321 cattgcgaat gcaatagtga ccattgtgga caatggcaaa tgcgcagcgc aagcgttgac
     1381 ggcgtctgcg aattccgtga cggtgcgtgc caagacagcg accattgacg ctacctcggc
     1441 ccaagtgttg atctatggcg gctctggaac ctacgcgatc accagtacca actcgggtgt
     1501 tgcatctgcc gccattgccg gcaatgtgct gaccataaag gggggtgact acactggaac
     1561 ggccagcgta acagtgtatg accagcagca acccaccttc tcggtgacaa ttaccgtgac
     1621 cgcgaactga cggccacatc tgcgcaggct aggcttgccc agttcttgtc cccatcctcc
     1681 ccggcttgcc cggggatttt tttacgccca gcgtgggcgg atcatttacg cggggggagt
     1741 ccggaaaagg gtaggtgcgc tcctacgcaa aaatggcgtc ggtcaccatc tggaatcaca
     1801 acgccggcct tcatcaggcc agcccactaa aactccaggt tgtcaatcag ccgcgtgccc
     1861 cccagccgcg ccgcgcccag ggtcaccagg gtgccggttg cggtgccgga ctgcggcggc
     1921 tgcaggtcgt gccgctggcg cacggtcagg tagtcggggg cccagccctg ggcctgcagg
     1981 gcctgcatgg cccgctcttc caactggggc agcggtgcgc tgcccgccag cgcggcctgg
     2041 gccagagcct gcagggcttg ggacagctgc acggcctggg tgcgctgccc ggcgtccagg
     2101 tagccgttgc gcgagctcag cgccaggccg tcgctggcgc ggctggtgtc gccggcgact
     2161 atgtcgaccg gcagcgcaaa ctgctccacc atgcggcgta tcaccatcag ttgctggtag
     2221 tccttcttgc cgaacacggc cgtgccgccg ccgcagggca gcagcacggc ggcaaacagc
     2281 ttcatgacca cggtgcagac gccggtgaag aagccggggc ggaactggcc ttccaggatg
     2341 tcggccaggg ccgggtcggg ctggatcttg aaggtctgcg gctcggggta caggtcgcgt
     2401 tcgcgcggtg caaagacgat gtcgcagtcg gcggcctcca gccgggcgca atcggcgtcc
     2461 catgtgcgcg ggtagctatc aaaatcttcg tgtggcagga actgcaggcg gttcacgaag
     2521 atgctggcaa cgagcgtgtc ggccagtggc ctggcctggc gcaccagggc aatgtggccg
     2581 tcgtgcaggt tgcccatggt gggcacgaag gcggggcgct ggcgcccggc gagggcggcg
     2641 cgcagttcgg ggagggtgcg ggcgatttgc atgggggagt ggtgatgttt accaggcgtg
     2701 caggctgtcg tccgggaagc tgccgttctt gacggcctgc acataggcct cgatggcgcc
     2761 gcgcacgctg gcggcgccct gcatgaagtc gcgcacgaac ttcgggttct tgcccaggtt
     2821 cacgcccagc atgtcgtgca ttaccagcac ctggccggcc gtgcccttgc ccgcgcctat
     2881 gccaatggtg tggcagtgcg gcagctcggc cgtcaggctg gtggaaagct gcgcgggcac
     2941 catttccagc accagcatgg cggcgccggc gtcctgcagc tccagtgcgt ggcggcgcag
     3001 ctcggcggcg gccgcgtcgc ccttgccctg cacgcggtag ccgcccaggg cgtgcacggt
     3061 ctgcggtgtc aggcccaggt gggcgcagac ggggatgccg cgctcgacca gaaactgcac
     3121 cgtgggcgcg gtccagccgc cgccctccag cttgaccatg tgcgccccgg cctgcatcag
     3181 gcggctggcg ctgcgcaatg cctgctcgcg gctttcgtgg tagctgccaa agggcaggtc
     3241 ggccaccacc caggccgtgc cctgcacgcg gtgcaggccg cgcgtcacgc tctcggtgtg
     3301 gtaggccatg gtgtcgagct gcacgcccac ggtgctgggc aggccctggc agaccatgcc
     3361 cagcgagtcg ccgaccagga tgcattccac acccgcggcg tcggccacgg cggcaaaggt
     3421 ggcgtcgtag gccgtcagca tggcgatctt ctcgccggcc tcgcgcatct gcgccaggcg
     3481 gggcaggctg atggggcggc gctggggcag gggcgaggcc gggggcaggg tgccgtaggg
     3541 cgtgccggtg gcggtggtgg tctggctcat ggtgcggtgg cggatgggct gagaatccgg
     3601 cgagtctagg cgcattggcc ggggtgggtg gcgcgatgtg gcttcgggta cacctggggg
     3661 ggccttgttt gcttcgtttt aaatagctgt tcgcgcttta tgagcgggcg tttgtggcaa
     3721 ttttgattca ataattgtca ctattccaag aactgctgcg tccaccccgg ccgcgctggc
     3781 ctgcaccagg caacagccac ccaggccttg ccagctttgc gctgcggcta tccttatgcc
     3841 ccccatgacc ctagccctgt gattttgcga tgactcctga atctattgcc gccctcgccc
     3901 gcgccgatgt ggcccgcgcc ctgcaggaag acctgggcag tggcgacctg accgctgccc
     3961 tggtgcccga gggccggcgc tgccgggcgc gcatcctggc gcgcgaagct gccgtgatct
     4021 gcggtgcgcc ctgggccgag gccgcgctgc gtgcgctcga ccccaccgtc agcctgacct
     4081 ggctggtggc cgagggccag cgctgcgccc ccgaccaggt ggtgctcgac attgcgggcg
     4141 atgcgcgcgc gctgctctcg gccgagcgca cggcgctcaa ctttctgcag ctgctgtccg
     4201 ccgtggcgac caagaccgcc atctatgtcg aggccgtcaa gggcacgcgc gcggccatcg
     4261 tggacacgcg caagaccatt cccggcctgc gcctggcgca aaagtacgcc gtgcgcgtgg
     4321 gcggcggggt gaaccaccgc ataggcctgc acgacgcggt gctgatcaag gaaaaccaca
     4381 ttgccgccgc cggcggcgtg acggcggtac tgcgcgcggc cgaggccgtg gcggcgcgcg
     4441 ccgccttcat cgagatcgag gtggagaccc tggcgcagct ggccgaggcg ctggacgccg
     4501 gcgcgaagat ggtgctgctg gacaacatgg atctgcccac gctgcgccag gcggttgaaa
     4561 tcaacgccgg gcgcgccatc ttggagatat ccggcggcgt cacgcaagat ggcttgcgcg
     4621 cgctggcgga gaccggcgtg gatcgcatct ccataggcgc gctgaccaag gatgtgaagg
     4681 ccacggattt ctcgatgcgc ctgcaggagc tggcatgaac accacgacct tgaaagaagt
     4741 cgagtacgag cagcccgagg gcggcgccgt ctgttccacc aagtacgcct gggcgcgcgt
     4801 gccgcccgag cccgcgcccg atgagcgtga gcggctcaag gccaggatcc gccgcctgct
     4861 gaaggagaag aacgccgtca tggtgtcgca ctactacgtg cacccagacc tgcaggatct
     4921 ggccgaggag accggcggca tcgtcagcga ctcgctggag atggcgcgct tcggccgcga
     4981 ccatgcggcg cagaccctgg tggtcagcgg cgtgcgcttc atgggcgaga cggcgaagat
     5041 cctgagcccc gagaagaccg tgctcatgcc tgacctggac gccacctgct cgctggatct
     5101 gggctgcccc atcgacgaat tcagcgcctt ttgcgaccag cacccggatc gcacggtggt
     5161 ggtctacgcc aacaccagcg cggccgtgaa ggcgcgctcg gactggctgg tcacatcgag
     5221 ctgcgcgctg gacatcgtgc gcgcgctgaa ggacaagggc cacaagatcc tgtgggcgcc
     5281 ggatcggcac ctgggcgact acatccgccg cgagacgggc gccgacatgc tgttctggaa
     5341 cggcgcctgc atcgtgcatg acgagttcaa ggcgctggag ctggagcttt tgatcaagga
     5401 gcacccgcaa gccaaggtgc tggtgcaccc cgagagcccg gccgaggtca tcgccctggc
     5461 cgacgccgtg ggctcgacca gcgccatcct gaaggccgcc agcgagatgg atgcgaccga
     5521 gttcatcgtc gccaccgata agggcatgat gcacaagctg cgccagctca acccgggcaa
     5581 gcagttttat gaggcaccga cggccggcaa cagcgccacc tgcaagagct gcgcccattg
     5641 cccctggatg gcgatgaatg gcctggccga cgtggcccgg gtgctggaga ccggcgccaa
     5701 cgccgtgcat gtggatccgg ccatcatccc gcgtgcgcgc atgcctatcg accgcatgct
     5761 ggcctttacc gccgccctca agaacggcca gcccgctggc gcgctggtgc cgcactttgg
     5821 tgctgcatga tgctattgat agtatagctg ttggcgcttg atgggtgtgt gctagcaggc
     5881 atttcccctg tatttttcac ggactcagtc ttgacttgag tcatgattgc ttacgtccgt
     5941 tgcagtggtg tgcgcattcc gcacgacgac gcccggcagc caggcgcata gtcgcctgtt
     6001 gaaatgctgg ggcagaaatg tcaatcggca tttcctgtgt cgtgtgaaaa gaaaggatgt
     6061 gatatgcgca atcgatctgc acttttgacg gctgcgctgc ttggactggc tggctccgcg
     6121 ttggcccaga gtggcgactc cacggtaaac cgctcggcgc aggtcgccag ccccggcctg
     6181 agccgtgccg aggtgctggc cgacctcgaa ctctggcaac gcgccggcat gacctattgg
     6241 ccgcacccgc cggccgagat ggacatgcag ttcacggccg aataccaggc cggcctggcg
     6301 cgctaccggc aactgcgcgg cggccaggcc atgcagcaga ccgcggccag gtacccggac
     6361 atgcccggca agtgatcggg caacagcttg gcttcaacca taggcgcccg cggttgcggg
     6421 cgcttttttg acggtgatgc ccgccgttga tttactattg aattagaagc gtcttgcgca
     6481 ataccggtaa gcgatcaacg ctattttcaa ttgaactcca tcatcgactt ccccgaccca
     6541 cacgacacgg cagccgcgcg gctgtgccat gcgttcggcc agccgctgcg cacgctggcg
     6601 gcctggggtc ccggcgaggt gcgcggcctg ctggacgcgg tgcagcaggc ggcccaggca
     6661 gggctgtggt gcctgggctg gctgcgctac gaggccgctg cggcctttga cacggcctat
     6721 gccgatgcgc tgcatgccac gcccgagggg cggccgctgg cctggtttgc cgtacatgag
     6781 ccacccgcag gcgccgcgca gagcagcttc gccgcacagg ccggggaggc gccgcgggtg
     6841 ttctggcagc cgggcctgga gcgcgcggcc ttcgatgccg ccatcgcgcg catccatgcg
     6901 gcgatcgcgg ccggcgactg ttaccagatc aactacacgg cgcagcttgc cgggcggctg
     6961 ctgggggacg cccaggcgct gtttgccgcg ctgcggcgcg cccagcccgg cggctatgtg
     7021 gcctgcctgg acgatggcgt ggagcaggtg ctgtcggtct cgcccgagct gttcttcgac
     7081 tgggacggcg agcgcatcct gacgcgcccg atgaagggca cggccgcgcg cggcgccacg
     7141 ccggcgcagg acgcggcgct ggcacaggcc atgcgtgcct cgcccaagga gcgggccgag
     7201 aacgtgatga tcgtggatct gctgcgcaac gacctgtcgc gcatcgccgt ggcgcacagc
     7261 gtgcgcgtgc cgcggctgtt ccatgccgag cagcttccca cggtgtggca gatgacctcg
     7321 gacgttgagg cccgcaccag gccgggcata ggcctggcgg atgtgttcgc ggcgctgttt
     7381 ccctgcggct ccatcaccgg cgcgcccaag cgccaggcca tgcggctgat ccgcgaactg
     7441 gagcccgggc cgcgcggcat ctactgcggc gccgtcggcg tggtgcgccc gggcagcgcg
     7501 ccggggcggg tgcacgccac cttcaacgtg ccgatacgca ccgtggtcgt gcgcggtgac
     7561 gaactgcgct gcggcatagg cagcggcatt acggcggatg cccgggccga ggccgagtgg
     7621 caggaatggg ccgcgaaacg cgcttttttg cgggggcttg gatgacggac tttcagatcc
     7681 tggaaaccct ggcgctggag cagggcgtgg cgcgccacct tgatcggcac ctggcgcgca
     7741 tggcggcgag cagcgcgcat ttcggctacc cctgggacga ggcgcgcgtg cgtgcctgcc
     7801 tggccgatct ggccgcgggc cacgccagcg gcctgtggcg cgtgcgcttg ctgctggatg
     7861 gcgccggcaa cccgcaggcc caggccttcg ccctgcagcc cacggcccag ccggtgcggc
     7921 tgcagctggc agaccggccg ctgcaagagg cgcatgacga gtgggtgcgc cacaagacga
     7981 cgcggcgcgc tcactatgcg gcgtttgcgc cggcgccggg cctgttcgac accgtgctct
     8041 acaacgcggc cggcgaggtc accgaatcca cctttggcaa catcgccgtg ctgctggacg
     8101 gccgctggct cacgccggcg ctctcctgcg gcctgctgcc cggcgtgggc cgggcggtgg
     8161 cgctggagca gggccgcgtc gaggaggcgg tgctgcgcct ggccgatctg ccgcgcgtgc
     8221 aggcctgggc cttcatcaac agcctgcgcg gctggctgga agcgcgtttg gcggcgcccg
     8281 gatagctatt tatttaatag ctgtctgcgc tttgtggatg ggcgtaaagg actgatttgt
     8341 ttgactagac acatccctca gactgggccg caagccctca cccccgccct ctcccagagg
     8401 gagagggagt aaagccggcg caaccggtcg ccctcgcccc tttggggaga gggaggggcg
     8461 aggggcatct gcaacatgta catagccaag ctgatttgac ttgaaatcaa agccgctctt
     8521 tcacatagga cggcctgtcc atgtccacga tctccaccga cagctgcacc atcacccccg
     8581 ccgggcgcgg cgtgaggcgg cgcagcacct cggccacgcg ggcggccagg tcctgcttgg
     8641 cctcggggct gcggcccgac agcaggcgca gctgggcatg cacgaagccg cggcccgagg
     8701 ccacggtgcc gacatggaag ctggcgttgc gcacgaagcg gctcttgagg tcggcctcgt
     8761 ccagcacctc ggggctggcg cagacggcgg cgttgagctc ggccagcgcc tgggcctcgg
     8821 ggtaggc
//
LOCUS       DAOMTQ010000125         8824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1667_length_8824_cov_29.362185, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000125 DAOMTQ010000000
VERSION     DAOMTQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1667_length_8824_cov_29.362185"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK518_11645"
     CDS             complement(<1..342)
                     /locus_tag="PK518_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HRM49613.1"
                     /translation="MGFAKPSLKARALRLLAQREHSRSELEAKLAPHVQEGDDLATLL
                     DQLQARDFINEERVVESVLHSRAPRLGAQRVLQELRRKGLDEDLLRAAAGRLKDTELE
                     RARAVWHQRFGN"
     gene            complement(414..1517)
                     /gene="recA"
                     /locus_tag="PK518_11650"
     CDS             complement(414..1517)
                     /gene="recA"
                     /locus_tag="PK518_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRM49614.1"
                     /translation="MDAPVKTADNSEKAKALQAALAQIEKQFGKGTIMRLGEGEQAQD
                     IQVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKLGGTCAFID
                     AEHALDTQYAQKLGVNLNDILISQPDTGEQALEIVDSLVRSGAVDLIVIDSVAALVPK
                     AEIEGEMGDALPGLQARLMSQALRKLTATIKKTNCMVIFINQIRMKIGVMFGSPETTT
                     GGNALKFYASVRLDIRRTGTIKKGDEAIGNETRVKVVKNKVSPPFKQAEFDILFGEGI
                     SREGEIIDMGVTARILEKSGAWYAYNGEKIGQGRDNAREFLRENPELAREIENKVRDS
                     LGIALLPGAAPAAAPDDKPARGKKGDKTDAAEG"
     gene            1656..2132
                     /locus_tag="PK518_11655"
     CDS             1656..2132
                     /locus_tag="PK518_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRM49615.1"
                     /translation="MHLPPHDDDWRQTHLGRLLGHAMRRFDARVLQLMARDVQVPLAL
                     SNLAAREQVSAAHIHITRHLQLSGDRLTDLADRAGMTKQSMASLVDQCEAWGLVTRAA
                     HPHDARARLVRFTPTGLAWLQGFRAAVAQAEAEFREAVGPDVATVVALGLEAYANG"
     gene            complement(2135..2608)
                     /locus_tag="PK518_11660"
     CDS             complement(2135..2608)
                     /locus_tag="PK518_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRM49616.1"
                     /translation="MSELDSIDRRILDLLQQNGRLTMTELGEQVGLSTSPCSQRVKRL
                     EAQGVITGYHARVNPAALGRSLLVFVEITLAQKSEQIFKKVRDELQNMPEVLECHLIS
                     GSFDYLVKARLAGMSEYRSLLGSILQQMPVPAQSNSYVVMEEVKESFVVPLGRRP"
     gene            2735..4042
                     /locus_tag="PK518_11665"
     CDS             2735..4042
                     /locus_tag="PK518_11665"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HRM49617.1"
                     /translation="MKVVVLGSGVIGVTTAYYLARAGAEVTVIDRQDGPALDTSFANA
                     GQVSFGYSTPWGAPGIPMKALKWMFEKHAPLSIRPDGSLFQLRWMAQMLRNCSTERYA
                     VNKERMLRLSNYSRQCIAQLRADTGIEYEGRQAGTLQLFRSQKQVDQAQRDIAVLQEC
                     GIDYELLDPQGVARAEPALARADATIAGGLRLPADETGDCFKFTNQLAQMAEALGVKF
                     RFGTAVDWLLFDGQGAVNGVQLSGPQREVVKADRYVLAMGSYSRDLLLSSGLDIPVYP
                     VKGYSLTIPIRDEALAPVSTVLDETYKIALTRFDNRIRVGGMAELSGFDKNLRPQRRA
                     TLEMVTSQLFPGGDLARAEFWTGLRPMTPDSTPIVGRTPHKNLFLNTGHGTLGWTMAC
                     GSGRLLADIVSGKATEISTEGLSVDRYLGTAQSTHRRTAGQAA"
     gene            4039..5100
                     /gene="alr"
                     /locus_tag="PK518_11670"
     CDS             4039..5100
                     /gene="alr"
                     /locus_tag="PK518_11670"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRM49618.1"
                     /translation="MSRPALATFDLAALRANYAHARAVHGGRALAVLKANAYGHGAVE
                     CARALAGDADAFAVAFTEEALALREAGIQQPILVLEGAFGMDDMRLAQAHGLWLVVHQ
                     QEQLRMLESLRAGARVNAWLKIDTGMHRAGLEPGEAQACWQRLKACEQVAGITLMTHL
                     ASADEPDNPVTARQLAAFEAATAGLPGPRSVANSAGVLAWQGAHRDWARPGIMLYGAD
                     PRGTPSDALQPVMQLTSEVFNVRTIGAGEALGYGGSFVAERPTRVGLVAMGYADGYPR
                     RAHTGTPVAVGGHLTRIIGRVAMDMLTVDLTDLPEVGVGAPVELWGRQVSVNSVAAAA
                     GTIAYELLCNVKRVRRVYR"
     gene            complement(5738..6475)
                     /locus_tag="PK518_11675"
                     /pseudo
     CDS             complement(5738..6475)
                     /locus_tag="PK518_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            6513..7394
                     /locus_tag="PK518_11680"
                     /pseudo
     CDS             6513..7394
                     /locus_tag="PK518_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            7470..8144
                     /locus_tag="PK518_11685"
     CDS             7470..8144
                     /locus_tag="PK518_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM49619.1"
                     /translation="MRILIAEDDQVLADGLLRTLRGSGAVVDHVASGSEADTALLTNN
                     EFDLLILDLGLPRLHGLEVLKRLRGRGSALPVLILTAADSIEERVKGLDLGADDYMAK
                     PFALSELEARVRALTRRGMGGTSATIKHGPLVYDQAGRVATIDGKMVELSARELGLLE
                     VLLQRAGRLVSKEQLVERLCEWGEEVSNNAIEVYIHRLRKKIEKGPIRIATVRGLGYC
                     LEKIPG"
     gene            8211..>8824
                     /locus_tag="PK518_11690"
     CDS             8211..>8824
                     /locus_tag="PK518_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HRM49620.1"
                     /translation="MKIFQREQRSLFGEILDWMLTPLLLLWPVSLALTWLVAQGLANK
                     PFDRALEYNAHALAQLVTVQNGRVLFNLPQPASEILRADESDIIYYQVLGPKGELLSG
                     ERELPLPPPPEERSFSGDVRLRDAELRGIDIRVAYIWVRVPLPGAPAALVQVAETRSK
                     RSVLATEIIKGVMLPQFVILPLAVLLVWLALVRGIKPLRQLEER"
BASE COUNT         1408 a         2935 c         2942 g         1539 t
ORIGIN      
        1 attgccgaag cgctggtgcc aaacggcacg ggcgcgctcc agttccgtgt ctttcaggcg
       61 cccggccgcg gcgcgcagca aatcctcgtc cagccccttg cggcgcagct cctgcagcac
      121 gcgctgtgcc ccaaggcgcg gtgcgcggct gtgcagcacg gattcgacga cacgctcttc
      181 gttgatgaag tcccgggcct gcagctggtc aagcagggtg gcgagatcat cgccctcctg
      241 cacatggggc gcgagcttgg cctccagctc gctgcgcgaa tgctcgcgct gggccagcag
      301 gcgcagcgcg cgtgccttca gagagggctt ggcaaatccc atgattacta tttaaacaat
      361 agctatcagc gctttgtata taagcgctga atgcctgttt gtgtgttggt ggatcagcct
      421 tcggcggcgt cggtcttgtc gcccttcttg ccgcgcgcgg gcttgtcgtc gggggctgcg
      481 gccggcgcgg cgccgggcag cagggcaatg cccaggctgt cgcgcacctt gttctcgatc
      541 tcgcgcgcca gttccgggtt ctcgcgcaga aattcgcgcg cgttgtcgcg gccctggcct
      601 atcttctcgc cgttgtaggc gtaccaggcg ccgctctttt cgaggatgcg cgccgtcacg
      661 cccatgtcga tgatctcgcc ctcgcgcgag atgccctcgc cgaacaggat gtcgaactcg
      721 gcctgcttga acgggggcga gaccttgttc ttcaccacct tcacacgggt ttcgttgcct
      781 atggcctcgt cgcccttctt gatggtgccg gtgcggcgta tgtccaggcg caccgaggcg
      841 tagaacttga gcgcgttgcc gccggtcgtc gtctcggggc tgccgaacat cacgccgatc
      901 ttcatgcgga tctggttgat gaagatgacc atgcagttgg tcttcttgat ggtggcggtg
      961 agcttgcgca gcgcctggct catcagccgc gcctgcaggc cgggcagggc gtcgcccatc
     1021 tcgccttcga tctcggcctt ggggacgagg gcggcgaccg agtcgatcac gatcagatcc
     1081 acggcgcccg agcgcaccag gctgtccacg atctccagcg cctgctcgcc ggtgtccggc
     1141 tggctgatca ggatgtcgtt caggttcacg cccagcttct gtgcgtactg cgtgtccagc
     1201 gcatgctcgg cgtcgatgaa ggcacaggtg ccgcccagct tctgcatctc ggcgatgacc
     1261 tgcagggtca gagtggtctt gccggaggac tccgggccgt agatctcgac cacgcgcccg
     1321 cgcggcaggc cgcccacgcc cagggcaatg tccaggccca gcgagccggt ggagacgacc
     1381 tggatgtcct gcgcctgctc gccctcgccc aggcgcatga tggtgccctt gccaaactgc
     1441 ttctctatct gggccagcgc ggcctgcagg gccttggcct tttcgctgtt gtcggcggtc
     1501 ttgacgggtg cgtccatgaa attgctcctc ttggtggcca tagtgctttc gggtaagact
     1561 gtttcgatca acagtctgta tgcgtgaaca gtagtttagg agggctggat tgtacttgga
     1621 agatattttt ggtcagtttg gctgacaata tcgccatgca tctgccaccc catgacgatg
     1681 actggcgcca gacccacctg ggccgcctgc tgggccatgc catgcggcgc tttgatgcgc
     1741 gcgtgctgca gctgatggcg cgcgacgtgc aggtgccgct ggccctgtcg aatctggcgg
     1801 cgcgcgaaca ggtgagtgcg gcgcatatcc acatcacacg ccacctgcag ctttcgggcg
     1861 accggttgac ggacctggcc gatcgcgccg gcatgaccaa gcagtccatg gccagcctgg
     1921 tcgatcaatg cgaggcctgg ggcctggtga cgcgcgcggc ccatccgcat gacgcgcgcg
     1981 cccggctggt gcgcttcacg cccacgggcc tggcctggct gcagggtttt cgcgccgccg
     2041 tggcccaggc cgaggccgag ttccgcgaag ccgtggggcc ggatgtagcc accgtcgtgg
     2101 ccctggggct ggaggcctat gcgaatggct gatatcaggg cctgcggccc aggggcacga
     2161 caaagctctc cttgacctct tccatcacca catagctgtt ggactgcgcc ggcaccggca
     2221 tctgctgcag gatggagccg agcaggctgc ggtactcgga catgccggcc agccgcgcct
     2281 tcaccaggta gtcaaagctg cccgagatca aatggcattc cagcacctcg ggcatgttct
     2341 gcagctcgtc gcgcaccttc ttgaagatct gttcggactt ctgcgccagg gtgatttcga
     2401 cgaacaccag caggctgcgc cccagcgcgg ccgggttcac gcgcgcgtgg tagccggtga
     2461 tcacgccctg ggcctccagg cgcttgacgc gctgcgagca gggcgaggtg gacaggccca
     2521 cctgctcgcc cagctcggtc atggtcaggc ggccgttctg ctgcaaaaga tcgaggatgc
     2581 gccggtctat ggagtcgagt tcagacattg ctgtgtgggt cagggtttgt ggccgtaaac
     2641 agcaaatatt gctgtcatag agcagaattt tggcaaaaga ttagcatgaa aatccatata
     2701 gtcacgatca ttcatcgaac atcggagaca gatcatgaaa gttgttgtac tcggctctgg
     2761 cgtgatcggc gtgaccaccg cctactacct ggcccgtgcg ggcgccgaag tcaccgtcat
     2821 cgaccgccag gatggcccgg cgctcgatac cagctttgcc aatgccggtc aggtctcgtt
     2881 cggctactcc acgccctggg gcgcacccgg catccccatg aaggcgctca aatggatgtt
     2941 cgagaagcat gcaccgctgt ccatccgccc cgatggcagt ctgttccagc tgcgctggat
     3001 ggcgcagatg ctgcgcaact gctcgaccga gcgctacgcc gtgaacaagg agcgcatgct
     3061 gcgcctgtcc aactacagcc gccagtgcat cgcccagctg cgcgccgata ccggcatcga
     3121 gtacgagggc cgtcaggcag gcacgctgca gctgtttcgc tcgcaaaagc aggttgacca
     3181 ggcgcagcgc gacattgccg tgctgcagga gtgcggcatt gactatgagc tgctcgaccc
     3241 ccagggcgtg gcgcgcgccg agccggccct ggcccgggcc gacgccacga tcgccggtgg
     3301 cctgcgcctg cccgcggacg agaccggcga ctgcttcaag ttcaccaacc agctggcgca
     3361 gatggccgag gccctgggcg tgaagttccg ctttggcacc gcagtggact ggttgctgtt
     3421 cgacggccag ggcgccgtca atggcgtgca gctgtccggc ccgcagcgcg aggtggtcaa
     3481 ggccgaccgc tacgtgctgg ccatgggcag ctattcgcgc gatctgctgc tgtcctccgg
     3541 cctggacatc ccggtgtacc cggtcaaggg ctattcgctg accatcccga tccgcgacga
     3601 ggccctggcg cccgtgtcca cggtgctgga cgagacctac aagatcgccc tgacgcgctt
     3661 tgacaaccgc atccgcgtcg gtggcatggc cgagctctcg ggctttgaca agaacctgcg
     3721 cccgcagcgc cgcgccacgc tggagatggt caccagccag ctgttccccg gcggcgacct
     3781 ggcgcgcgcc gaattctgga ccggcctgcg ccccatgacc cccgacagca cgcccatcgt
     3841 cggccgcacg ccgcacaaaa acctgttcct gaacaccggc cacggcacgc tgggctggac
     3901 catggcctgc ggctcgggcc ggctgctggc cgacatcgtc agcggcaagg ccacggagat
     3961 ttccaccgaa ggcctgtccg tcgatcgcta cctgggcacg gctcagagca cgcaccgccg
     4021 caccgcgggt caggccgcat gagccgcccg gcactggcga cattcgatct agccgcgctg
     4081 cgcgccaact atgcccacgc cagggctgtc catggcggcc gggccctcgc cgtcctcaag
     4141 gccaacgcct acggccatgg cgcggtggaa tgcgcgcgcg ccctggcggg cgatgccgac
     4201 gcctttgccg tcgctttcac cgaggaagcg ctggcgctgc gcgaggccgg catccagcaa
     4261 cccatcctgg tgctcgaagg cgccttcggc atggacgaca tgcggctggc ccaggcgcat
     4321 ggcctgtggc tggtggtgca ccagcaggaa cagctgcgca tgctggaatc gctgcgcgcc
     4381 ggcgcgcgcg tcaacgcctg gctcaagatc gacaccggca tgcaccgcgc gggcctggag
     4441 cccggcgagg cccaggcctg ctggcagcgg ctcaaggcct gcgagcaggt ggccggcatc
     4501 acgctgatga cgcacctggc ctcggccgac gagccggaca acccggtcac cgcccggcag
     4561 ctggccgctt tcgaggctgc caccgcgggc ctgcccgggc cgcgcagcgt ggccaattcg
     4621 gccggcgtgc tggcctggca gggcgcgcac cgcgactggg cgcggcccgg catcatgctc
     4681 tacggcgccg atccgcgcgg cacgccatcg gatgcgctgc agccggtgat gcagctgacc
     4741 agcgaggtct ttaacgtgcg cacgattggc gctggcgagg ccctgggcta cggcggcagc
     4801 ttcgtcgccg agcgccccac acgcgtcggc ctggtggcca tgggctacgc cgacggctac
     4861 ccgcgccgcg cgcacaccgg aacgccggtg gcggtgggtg gtcacctgac gcgcatcatc
     4921 ggccgcgtgg ccatggacat gttgaccgtc gatctgacgg atctgccaga ggtcggcgtg
     4981 ggcgctccgg tggagctgtg ggggcggcag gtcagtgtga acagcgttgc ggcggcggcg
     5041 gggacgattg catatgagct gctgtgcaat gtgaagcggg tgcggcgggt gtatcgctag
     5101 tttttttgag tgtggctggc cgggatatgc gcccggcggc gcacctactt tcttttgctt
     5161 cgccaaaaga aagtaggcaa agaaaaggcg accctgctgt ccgtgacccc tccgcttcgc
     5221 tacggggcaa cctgtggtgc tcgcgcatgg ggtgcgccgt ggaactcact gcgcgcctgc
     5281 ggcgcttcgt tcaaacaacc acggcgagtc agtttaagaa gcatgggcgc ttcgacgccc
     5341 atgcacaccc catgcgctgt gctcctcggc acggccagaa gggagggggg acgacacggg
     5401 ccatcgctgc gctcggcctc gtgaggggcg cgccatttgt cgggccgagc gcagcgatgg
     5461 cccgtgtcgg gctgttcacc ccccctgtgg ctgcgccaca ggtttgcccc gtagcgcagc
     5521 gaaggggtcg cagacttggg ggcgtgttct ttgcctcctt tcttgcacga gcaagaaagg
     5581 aggtcgcccg ccggggcgag acccggccag cgacatcagc aatgtaaaga accacaagag
     5641 caatcaccgt gcgtcgtgag tcctgccgtt ttcaggccgt gcaggcccct cacccctccc
     5701 tctccccaaa ggggcgaggg cgatccgtcg cgcaagatca ggccttgtcc gccttgcctg
     5761 ccgcattcgc aaaccggctc agctgcctgt ccagccacca gggcgcgcgt ccctcgtctt
     5821 ccgcggcttc cttgcgccgt atggcgttgc gcaccacgat ggagcccagg tagcggatgg
     5881 gttcgggcgg aaacagcccc agcggcccgc gcgtgagcgg actgtgcgtc caggcgttgt
     5941 cttcgtccag caccagggac gacaggattt gcccgcccat gtagctcggg cccacgccat
     6001 tgcccgaata gccgaagccg tagaacatgt tggcggcgcc gttcaggcgg ccaaagaagg
     6061 gaaaaccgct gacagagcgg tccgacggtc cgttccagct ggcagtgacc ggcaccccgg
     6121 ccagcgacgg gaagaaaccg tgcaacgcct gcgtgagttg cccctcgtac ggcgagcgct
     6181 gatcgaacac cggcgcgatg cgtccgccgc gggcaaaggt gttgccgccc ttgcccagca
     6241 tcaggcgtcc gtcctcggtg ctgcggtagt agtgcacgaa ggtgcgcgag tctagtaccg
     6301 atacgccatc gatcaggccc atggatcgca gcagctcggg gcagcgctcg gtgatgacca
     6361 tgtcgctgga gacgatggcg atgctgcgct cgaactgtgg caatgcctcg gccatccagg
     6421 cgttcagcgc cagcaccagc tttttcgccg tgacgctgcc gcagggcgtg cgcacccgga
     6481 cgctggcgcc agcgtcatga cccagcatgg atgtgctggt gccgaacaac ggggcctact
     6541 gcgcgcgcct ggcgcgcatt gtcaaggtgc tcgggcgcaa gctgacgacc atcgactaca
     6601 gagaggccca gcaggtcagc gccgccgacg tggacaaggc gctcgccgcc gatccgtcca
     6661 tcacccatgt cgccctggtg cactgcgaga ccggcgccgg cgtgctcaat ccgctgcacg
     6721 aggtggcgca ggtggtggcc aggcatggca agggcctgat cgtggacgcc atgagctcgt
     6781 tcggtgcgat cgacatcgac gccagcaaga ccccgtttga cgccgtcatt gccgcctcgg
     6841 gcaaatgcct ggaaggcccg ccgggcatgg gcttcgtcat cgtgcgcaag agcgtgctgg
     6901 aaaagtgcga gggcaacgcc cattcgctgg cgctggacct gtacgaccag tgggtctaca
     6961 tgcaaaagac cacgcaatgg cgcttcaccc cgcccacgca tatcgtcgcc gcgctggacg
     7021 aggccatgac gcaataccag gaggaaggtg gcctcgcggc gcgcggcggg cgctacgcgc
     7081 gcaactgcaa ggctctgctc gatggcatgc gcgccatggg ctttcgcagt tatctgccgg
     7141 acgatctgca ggcgcccatc atcgtcacct tccacgcccc ggccgatccg gcctatgagt
     7201 tcaaggcctt ctaccaggag ggcaagaagc gcggctacat cctctacccc ggcaagctga
     7261 cgcagctgga aaccttccgc gtgggctgca taggccattt cggtgacgtc ggcatccccg
     7321 gcgccgtggc ggcggtggcc gatgcgctcc aggccatggg cgtcaagcag atcagcgtcg
     7381 ccgagccggc ctgaggcgcc gcatgctcct agaatcggcc atgggcccgg cggtgttgcc
     7441 gcggcccaag gccttccagg agaacaccca tgcgcatcct catcgccgaa gacgaccagg
     7501 tgctggccga cggcctgctg cgcacgctgc gcggctcggg cgccgtcgtg gaccatgtgg
     7561 ccagcggcag cgaggccgat accgcgctgc tgaccaataa cgagttcgac ctgctgatcc
     7621 tggatctggg cctgcccagg ctgcatgggc tggaggtctt gaagcgcctg cgcgggcgcg
     7681 gctcggccct gccggtgctg atcctcaccg cggccgacag catcgaggag cgcgtcaagg
     7741 gcctggatct gggcgccgac gactacatgg ccaagccctt tgccctgtcg gagctggagg
     7801 cgcgcgtgcg cgccctcacg cgccgcggca tgggcggcac cagcgccacc atcaagcatg
     7861 gccccctggt gtacgaccag gccgggcgcg tggccaccat tgacggcaag atggtggagc
     7921 tgtcggcgcg cgagctgggc ctgctggagg tgctgctgca gcgcgccggc cgcctggtga
     7981 gcaaggagca gctggtggag cgcctgtgcg agtggggcga ggaggtgagc aacaacgcca
     8041 tcgaggtcta catccaccgc ctgcgcaaga agatagagaa gggcccgatc cgcatcgcca
     8101 ccgtgcgtgg cctgggctac tgcctggaaa agatccccgg ttgatgctat tttgttgata
     8161 gctgttggcg cttatccaat aagcgctaca ggcttaaaac acttgaaacc gtgaaaatct
     8221 tccagcgcga gcagcgctcc ctctttggcg aaatcctgga ctggatgttg acgccactgc
     8281 tgctcttgtg gccggtcagc ctggcgctga cctggctggt ggcccagggt ctggccaaca
     8341 agcccttcga ccgcgcgctc gaatacaacg cccatgccct ggcccagttg gtcaccgtgc
     8401 agaacggccg cgtgttgttc aacctgcccc agccggccag cgagatcctg cgtgccgacg
     8461 agtccgacat catttactac caggtgctcg ggcccaaggg cgagctgctc tcgggcgagc
     8521 gcgagctgcc gctgccgccg ccgcccgagg agcgctcctt ctcgggcgat gtgcgcctgc
     8581 gcgatgccga gctgcgcggc atcgacatcc gcgtggccta tatctgggtg cgcgtgcccc
     8641 tgcctggcgc acccgcggcc ctggtgcagg tggccgagac gcgctccaag cgcagcgtgc
     8701 tggccaccga gatcatcaag ggtgtgatgc tgccgcagtt cgtcatcctg ccgctggccg
     8761 tgctgctggt gtggctggcc ctggtgcgcg gcatcaagcc gctgcgccag ctggaagagc
     8821 gcat
//
LOCUS       DAOMTQ010000126         8796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1673_length_8796_cov_26.186020, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000126 DAOMTQ010000000
VERSION     DAOMTQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_8796_cov_26.186020"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1855)
                     /locus_tag="PK518_11695"
     CDS             complement(197..1855)
                     /locus_tag="PK518_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM49621.1"
                     /translation="MDMYALMRWFSIRMRMVGAIGVVLLLLALLGGAGMLGMFRIQGA
                     SQDFVAQPHAAVRLLGELRGEMGQIRQLEKDMIIHYEKVDAVRAAHQKWLAALDRAKD
                     LAAGFDRLGLAGPQTLAQDIARLLDGYRAQFAHVARQLEEGGYDSATIANRMSTKAVA
                     QFDEAAQKLQALDQALGAEADGAIARQAGIAEQTQWLFGLAVLITVVVVVPSTMLNMV
                     SICRPLEAARALAQSIASGDLSRRMAVEGRDEAADLQRALLDMQAGLGAIVAQVRDAG
                     GNISVASQEIASGNQDLSARTEQTASNAQQAVAALSQLTTTVQQTASSSQVASQLVAS
                     ASGQATRGGQVVQQAVASMRDIAASSHKIGDIIGLIDSIAFQTNILALNAAVEAARAG
                     EQGRGFAVVAGEVRSLAQRSAQAASEIKGLIQSSVQAVDGGVRHVEDAGAAMQEIVGS
                     VQRVTDIIGEINSAASEQASGIGAVNASVAEIDRMTQQNAALVEESAAAADSLREQAA
                     RLSQVVQQFRLADGARGAAAQQPGAIALAPAQSGQTARPRLGMA"
     gene            complement(1975..4293)
                     /locus_tag="PK518_11700"
     CDS             complement(1975..4293)
                     /locus_tag="PK518_11700"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM49622.1"
                     /translation="MTSATLSSTAAAQTLDLGVGGMTCASCVGRVERALRKVPGVQDA
                     TVNLATERAHVVYDPSTAPGMDAVLRRAVRNAGYEPRSAAEQDAGEALEQSPWAGFMP
                     VAIGLLLSAPLVLPMFGDLFGQHWMLPAWAQFLLATPVQFWLGARFYKAGWHAARALT
                     GNMDLLVAIGTSAGYGLSLWLWWTAEAGHAPHLYFEASAVVITLVLLGKWLEARAKRQ
                     TTAAIRALHALRPEVAHLLSRDGEVDVPIAEVMAGDRLVVRPGERIPVDGLVHEGHTQ
                     VDESMLTGEPLPVLREAGGQLTGGSINGDGRIVMQVTAVGAETVLARIIRLVEDAQAA
                     KAPIQRLVDKVSAVFVPTVIVIALVTLLGWLWVGVGLETALIRAVAVLVIACPCALGL
                     ATPAAIMAGTGVAARHGILIKDAEALEVAHRVRTVAFDKTGTLTVGRPRLTAFHVEPG
                     GDEAGVLAAVAAVQSGSEHPLARAVVAAAQERGLHVAQPEGVRAVPGRGTEGEVAGRS
                     YLVGSLRWMQELGVDLGPLRDAAAALQGQGATVSAVAERVTAGLAARALLAFGDEPKP
                     GARAALAALKAQGVRCVMISGDNRGAAESMARRLGLDPAAGEVLAEVLPGDKAAAVTA
                     LKADGHFERRRAAPRREGPPGGQRPSAAAERGGHVVAMVGDGVNDAPALAAADVGMAM
                     GNGTDVAMHAAGVTLMRGDPALVAAALDISHRTVAKIRQNLFWAFAYNVAGIPLAALG
                     YLSPVVAGAAMALSSVSVMTNALLLKRWSPDK"
     gene            4437..4634
                     /locus_tag="PK518_11705"
     CDS             4437..4634
                     /locus_tag="PK518_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRM49623.1"
                     /translation="MQHQFQVTGMTCGHCERAVVHAVRSIDEEALVKVDLPTGQVTVE
                     SDKARDAIATAIREEGYQVAA"
     gene            4631..5050
                     /gene="cueR"
                     /locus_tag="PK518_11710"
     CDS             4631..5050
                     /gene="cueR"
                     /locus_tag="PK518_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HRM49624.1"
                     /translation="MRAATAARWPVAIGQAAERAGVSARMVRHYEGLGLLAGVARTDS
                     GYRQYTEADVHSLRFIRRARDLGFSMDEIAQLLGLWQDKARASSQVKQIAQKHIDNLA
                     ERIAAMQAMQRSLQTLVGCCHGDERPDCPILDDLAAN"
     gene            5150..5929
                     /locus_tag="PK518_11715"
     CDS             5150..5929
                     /locus_tag="PK518_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRM49625.1"
                     /translation="MTQAPLLVTRQDAIATLLFNRPEAMNALDVPTARALLAAMQDIA
                     QDTSVRAVLLKGAGRAFVAGGDLATLQADPVQGAKDLLAPLNQALLLLQSLNAPVIAQ
                     VHGAAAGAGLSLMLMCDFVLAAEGTKFNLAYINLGTSCDVGASWALPRLVGLRRALEI
                     AMLGEGFGADEALRLGLINRLLPAAELEDAARAFAQRLASGPTLAYGHMRRLLRAGLD
                     HDLATQLQAEAHAFDACAHSHDLREGINAFLAKRRPQFSGK"
     gene            complement(5984..6373)
                     /locus_tag="PK518_11720"
     CDS             complement(5984..6373)
                     /locus_tag="PK518_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRM49626.1"
                     /translation="MAPSGPGGNTPGNTPASTPGSTPESAPQNPGPSNPQAVPPVLDL
                     DAPKGDPVITADAIPPGSAPDNFGPNDLPTTPPSLNPDDSLSDPVIPVGAQDPVAGPK
                     NTVAEPSSLALLGIAALGLVLRRRRRA"
     gene            6375..6965
                     /locus_tag="PK518_11725"
     CDS             6375..6965
                     /locus_tag="PK518_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49627.1"
                     /translation="MATSEGVLPVPVAGVAGLSAGEAPGPLIAGGLAEEPAAGDDAPD
                     GDRCMPGNGMALAACCAWRPEAADAPPLLAGAAPAPEAPTTRAAPAPVASPRLAPPLP
                     TGAEGAGARPTMVATMPGSLDVGTARLPRPASRSGVFDDPCCVALAFSGAAASRWSGA
                     DLPARMTDPVAMEPAPSTTATASAIFNMGFFMAANF"
     assembly_gap    7332..7431
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7484..7735)
                     /locus_tag="PK518_11730"
     CDS             complement(7484..7735)
                     /locus_tag="PK518_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HRM49628.1"
                     /translation="MTDSAQIPLPDHLAVDPRSPHHVAAVFQQDAEIGIRFNGKERFD
                     VEEYCVSEGWIKVPAGKTRDRKGRPLLITLKGVVEAFYK"
     gene            complement(8189..>8796)
                     /locus_tag="PK518_11735"
     CDS             complement(8189..>8796)
                     /locus_tag="PK518_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49629.1"
                     /translation="LAVGGLLAAAVAGLARLPAERAAQVAGTTGAAVILGVVLLFLLA
                     GTWRRVNMYDAFIDGAKEGFGVAVQIIPYLIAILVAIGVFRAAGCMDALLSAIGAGVA
                     TLGWNTDFLPALPVGLMKVLSGAGARGLMIDVLQTHGVDSFVGRLAAIIQGSTETTFY
                     VLAVYFGSVGVKHHRHALACALLADAVGLVAAIGVGYAMWR"
BASE COUNT         1461 a         3109 c         2822 g         1304 t
ORIGIN      
        1 ctccctctcc ctctgggaga gggtgggggt gagggctcgc ggcccagtct gagatatgtg
       61 ttttgtcagg caaaaagtgg ctcgagcgct cgcccatcaa gcgtcaacag ctatgaatta
      121 cggagtgaaa aaaagcgcac ccgagggtgc gccaagaggg gactgctgcg ctggcccggg
      181 ccagcatgat tcgtcgtcag gccatgccca ggcggggccg tgcggtctgc ccggactggg
      241 ccggggccag cgcgatggcg cccggctgct gcgccgccgc gccccgcgcc ccgtccgcca
      301 gccggaactg ctgcaccacc tgcgacagcc gcgctgcctg ttcgcgcagc gaatcggcgg
      361 cggcggcgga ttcttccacc agggcggcgt tctgctgcgt catgcggtcg atctcggcca
      421 ccgaggcgtt caccgcgcct atgccgctgg cctgctcgct ggcggcggag ttgatctcgc
      481 caatgatgtc ggtcacgcgc tgcacgctgc cgacgatctc ctgcatggcg gcgccggcgt
      541 cctccacatg gcgcacgcca ccgtccacgg cctgcacgct ggactggatc aggcccttga
      601 tctcgctggc cgcctgggcg ctgcgctgcg ccagagagcg cacctcgccg gccaccacgg
      661 caaagccgcg gccctgctcg ccggcgcggg ccgcctccac ggcggcgttc agcgccagga
      721 tgttggtctg gaaggcgatg gagtcgatca ggccgatgat gtcgccgatc ttgtggctgg
      781 aggcggcgat gtcgcgcatg ctggccacgg cctgctgcac cacctggccg ccgcgcgtgg
      841 cctggcccga ggccgatgcc accagctggc tcgccacctg cgaggaagac gccgtctgct
      901 gcaccgtggt ggtcagctgc gacagcgcgg ccacggcctg ctgggcgttg ctggcggtct
      961 gctcggtgcg tgccgacaag tcctggttgc cgctggcgat ctcttggctg gccaccgaga
     1021 tgttgccccc ggcatcgcgc acctgggcca cgatggcgcc caggccagcc tgcatgtcca
     1081 gcagcgcgcg ctgcaggtcg gcggcctcgt cgcggccctc gaccgccatg cgccgcgaca
     1141 ggtcgccgct ggcaatcgac tgcgccaggg cgcgcgcggc ctcgagcggg cggcagatgg
     1201 agaccatgtt cagcatcgtc gacggcacca ccaccaccac ggtgatcagc accgccaggc
     1261 cgaacagcca ctgggtctgc tcggcaatgc cggcctggcg cgcaatggcg ccatcggcct
     1321 cggcacccag ggcctggtcc agcgcctgca gcttctgcgc cgcctcgtca aactgcgcca
     1381 cggccttggt gctcatgcgg ttggcaatgg tggccgagtc gtaaccgccc tcctccagct
     1441 ggcgcgccac atgggcaaac tgcgcgcggt agccgtccag cagcctggcg atgtcctggg
     1501 ccagggtttg cggcccggcc agtcccaagc ggtcaaagcc cgccgccagg tccttggccc
     1561 ggtccagggc cgccagccat ttttggtgcg cggcacgcac ggcatcgacc ttctcgtagt
     1621 ggatgatcat gtccttctcg agctggcgta tctggcccat ctcgccgcgc agctcgccca
     1681 gcaggcgcac ggcggcatgg ggctgggcaa caaagtcctg gctggccccc tggatgcgga
     1741 acatgcccag catgcccgcc ccacccagca gcgccagcag cagcagcacc acgccaatgg
     1801 cgcccaccat gcgcatgcga atcgagaacc agcgcatgag cgcgtacata tccattgctg
     1861 tcttcctgtc gttcaagcgg cactgggtgc ggtgccgggg cgcctatctg caacaaattg
     1921 ctgcagatta ttggccagat tgacagttga ttgacaaatt attacttgtg gcgattactt
     1981 atctggcgac cagcgcttga gcaacaaggc attcgtcatc acgctcaccg agctcagcgc
     2041 catggccgcg ccggccacca ccgggctcag gtagcccagg gccgccagcg gtatgccggc
     2101 cacgttgtag gcaaaggccc agaacaggtt ctgccggatc ttggccacgg tgcggtgcga
     2161 gatatccagc gccgctgcca ccagcgccgg gtcgccgcgc atcagcgtca cgccggcggc
     2221 gtgcatggcc acgtccgtgc cgttgcccat ggccatgccg acgtcggccg ccgccagggc
     2281 cggtgcatcg ttcacgccgt cgcccaccat ggcgacgaca tggcccccac gctccgccgc
     2341 tgcgctgggt cgctgccccc caggggggcc ttcgcgcctt ggggcggccc ggcggcgctc
     2401 aaaatgccca tccgccttca aggccgtgac ggccgccgcc ttgtcgccgg gcagcacctc
     2461 ggccagcacc tcgccggcgg ccggatccag gcccaggcgg cgcgccatgc tttctgccgc
     2521 gccgcggttg tcacccgaga tcatcacgca gcgcacgccc tgggccttga gagcggccag
     2581 cgccgcgcgc gcgcctggct tgggctcatc gccaaaggcc agcagggccc gggcggccag
     2641 ccctgcggtg acgcgctcgg ccacggccga cacggtggcg ccctgccctt gcagcgcggc
     2701 ggcggcgtca cgcaatggcc ccagatcgac gcccagctcc tgcatccagc gcaggctgcc
     2761 caccaggtag ctgcggcctg ccacctcgcc ctcggtgccg cgcccgggca cggcgcgcac
     2821 gccctcgggc tgcgcgacat gcaaaccacg ctcctgcgca gcggccacca cggcgcgcgc
     2881 cagcgggtgc tcgctaccgc tctgtacggc ggccaccgcc gcgagcacgc ccgcctcgtc
     2941 gccacctggt tccacgtgaa acgccgtcag gcgcggccgg cccacggtca gcgtgccggt
     3001 cttgtcaaag gccacggtgc gcacgcggtg cgccacctcc agcgcctcgg catccttgat
     3061 caggatgcca tgcctggccg ccacgcccgt gccggccatg atggccgccg gtgtcgccag
     3121 gcccagcgcg caggggcagg cgatgaccag caccgccacg gcgcggatca gcgccgtctc
     3181 cagccccacg ccgacccaca gccagcccag cagcgtgacc agggcaatca cgatcaccgt
     3241 gggcacgaac accgccgaca ccttgtccac cagccgttgt atcggcgcct tggcggcctg
     3301 ggcgtcctcc accaggcgga tgatgcgcgc cagcaccgtc tcggcgccca cggccgtcac
     3361 ctgcatcaca atgcggccat cgccgttgat cgacccgccc gtgagctgcc cgcccgcctc
     3421 gcgcagcacg ggcaggggct cgccggtcag catggactcg tccacctggg tgtggccctc
     3481 atgcaccagc ccgtccaccg gaatgcgctc gcccgggcgc accaccagtc ggtcaccggc
     3541 catgacctcg gcgatgggca catccacctc gccgtcgcgc gagagcagat gcgccacctc
     3601 gggccgcagc gcgtgcagcg cgcgaatcgc cgccgtggtc tggcgcttgg cgcgcgcctc
     3661 cagccacttg cccagcaaca ccagggtgat gaccacggcc gaggcctcga agtacaggtg
     3721 cggcgcatgg cccgcctcgg ccgtccacca cagccacagc gacaggccat agcccgcgct
     3781 ggtgcctatg gccaccagca gatccatgtt gccggtcagc gcgcgggccg catgccagcc
     3841 ggccttgtag aaacgcgcac ccagccagaa ctgcaccggc gtcgccaaca agaattgcgc
     3901 ccaggcaggc agcatccaat gctggccgaa cagatcgccg aacatgggca gcaccagcgg
     3961 cgccgacagc agcaggccga tggccaccgg catgaagccg gcccagggcg actgctccag
     4021 cgcctcgccg gcgtcctgct cggcggcgct gcgcggctcg tagccggcgt tgcgcacggc
     4081 gcggcgcaac acggcgtcca tgccgggcgc agtgctcggg tcatagacga catgggcgcg
     4141 ctcggtcgcc aggttcaccg tggcgtcctg cacgccgggc accttgcgca gggcgcgctc
     4201 cacgcggccc acgcagctgg cacaggtcat gccgcccacg cccaggtcca gcgtttgcgc
     4261 ggctgcggtg gatgaaagag tggcagaggt catgtcgggc accttggaat caagcatggc
     4321 gctaccataa accttgacat gatggcaaag tcaagccacg cgcagcgctt gactttgaca
     4381 ccatgtcagg cttcatcatg caagcccatt caccccgcaa ccccgaaagg atcaccatgc
     4441 aacaccagtt tcaagtcacc ggaatgacct gcggccactg cgagcgcgcc gtggtgcatg
     4501 ccgtgcgcag catcgacgaa gaggccctgg tcaaggtgga cctgcccacc ggccaggtga
     4561 cggtggaaag cgacaaggcg cgcgacgcca tcgccaccgc catccgtgaa gagggctacc
     4621 aggtcgccgc atgagggccg ccaccgccgc gcgctggccg gtggccatag gccaggctgc
     4681 cgaacgcgcg ggtgtttcgg cgcgcatggt gcgccactac gaaggcctgg gcctgctggc
     4741 cggcgtggcc cgcaccgaca gcggctatcg gcagtacacc gaggccgacg tgcacagcct
     4801 gcgcttcatc cgccgcgcac gcgacctcgg tttctcgatg gacgagatcg cccagctgct
     4861 cggcctgtgg caggacaagg cccgcgccag cagccaggtc aagcagatcg cgcaaaagca
     4921 catcgacaac ctggctgaac gcattgccgc gatgcaggcc atgcagcgca gcctgcagac
     4981 gctggtgggt tgctgccacg gcgacgagcg gccggattgc cccatcctgg atgacctggc
     5041 ggccaactga gcacggcgtc ccgcaacggc cacaagaaaa cttggtcaaa atggcatgaa
     5101 gcgcttttcc gtcaagcgcc agccgctatg gattttcaag gaaccaccca tgacccaagc
     5161 ccctttgctc gtcacccgcc aggacgccat tgccaccctg ctattcaacc gccccgaggc
     5221 catgaatgcg ctggacgtac cgaccgcccg ggccctgctg gcggccatgc aggacattgc
     5281 gcaggacacc agcgtgcgcg ccgtgctgct caagggcgcg ggccgcgcct tcgtcgccgg
     5341 gggcgacctg gccaccctgc aggccgaccc ggtgcagggc gccaaggatc tgctggcgcc
     5401 gctgaaccag gctttgctgc tgctacaaag cctgaacgcc ccggtcatcg cccaggtgca
     5461 tggcgcggcg gccggcgccg gcctgtcctt gatgctgatg tgcgacttcg tgctcgccgc
     5521 cgaggggacc aagttcaacc tggcctacat caacctgggc accagctgcg acgtgggcgc
     5581 gtcctgggcg ctgccgcgcc tggtgggcct gcgtcgcgct ctggagatcg ccatgctggg
     5641 cgagggcttc ggcgccgacg aggccctgcg cctggggctc atcaaccgcc tgctgcccgc
     5701 cgccgagctg gaggacgccg cccgggcctt tgcccagcgc ctggccagcg gccccaccct
     5761 ggcctatggc cacatgcgcc gcctgctgcg cgccgggctg gatcatgacc tggccaccca
     5821 gctgcaggcc gaggcccacg cctttgatgc ctgcgcccac agccacgacc tgcgcgaagg
     5881 catcaacgcc ttcctggcca aacgcaggcc gcagttcagc ggaaaatgag aaccagtcaa
     5941 aacgccggca attgccggcg ttttgacatc agggtgatgg cgctcaggcc cgcctgcgcc
     6001 ggcgcagcac caggcccagg gcggcgatgc caagcagggc cagggacgat ggctcggcca
     6061 cggtgttctt cgggccggcg acggggtctt gcgcgccgac ggggatcacc gggtcactca
     6121 gggagtcatc cgggttcagc gatggcgggg tggtcggaag atcattgggg ccgaagttgt
     6181 ccggcgcgct gccggggggg atggcgtcgg cggtgatcac cgggtcgccc ttgggagcat
     6241 ccaggtccag caccggcggg acggcttgcg ggttgctggg gccggggttc tgcggtgcgc
     6301 tttcaggggt gctgccaggg gtactcgcag gggtattgcc aggggtgttg ccgccagggc
     6361 cggaaggagc caccttggca acctcggaag gcgtgctgcc agtgccggtt gcgggggttg
     6421 ccggcttgtc agccggcgaa gcaccagggc cgctgattgc cggtggcttg gccgaagagc
     6481 ccgctgccgg cgacgatgca cccgatggcg accgctgcat gccaggcaat ggcatggcac
     6541 tggcggcctg ctgcgcatgg cggcccgagg cagccgatgc gccgccactg cttgccgggg
     6601 cagcgcccgc ccccgaagcg ccgacaacac gggcagcacc cgcgcctgta gcctcacccc
     6661 ggcttgcgcc gccgctgcca acaggcgctg agggcgcggg cgcaaggccg acgatggtcg
     6721 caaccatgcc cgggtcgctc gacgtgggca ctgcccgctt gccacggcct gcctcgcggt
     6781 cgggcgtctt tgacgacccc tgctgcgtag cgctcgcctt ctcaggcgcc gccgcaagcc
     6841 gctggtcggg tgcggatctg cccgccagga tgaccgatcc ggtcgcgatg gagcccgcgc
     6901 caagcaccac ggcaacggcc agcgcaatct tcaacatggg ctttttcatg gctgcaaact
     6961 tctgatgtct acgtgatcaa ggatcggctt cggtggcttc gtgagactca cgaggctttg
     7021 tatgaaacat taagcaaaca tcatgcctaa aaataaactt atttaaaatc aacaaggtgg
     7081 cgataaatta agtttagcgg catcctcatt tgtaagaaaa actgacaaaa cgtagttgga
     7141 atcacggctc acccaaaagc atcggtgccg ttgctcgctg ctgtcgctta ccgaaccaca
     7201 tggcggccgg cgccagaaaa aacgccgact aatgccggcg tcttacatgc caatgactgc
     7261 cctcaggccc tcatgcgccg gcgtgccacc aagcccagcc ccactatgcc aagcagggcc
     7321 agggtcgagg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtctcacgc
     7441 gctgtaaaaa atcccgacaa atcacggccg cgctgcggcc ggcctacttg tagaacgcct
     7501 ccaccacgcc cttgagcgtg atcagcaatg gccgcccctt gcggtcgcgt gtcttgcccg
     7561 cgggcacctt gatccagccc tcgctgacgc agtactcctc gacgtcgaag cgctccttgc
     7621 cattgaagcg tatgcctatc tcggcgtcct gctggaacac cgccgccaca tggtgcgggc
     7681 tgcgcgggtc aacggccagg tggtcaggca gggggatctg ggcggaatcg gtcacggcaa
     7741 ggccttgcaa aaagaacagg ggccgcattg tcccgcatct ttctcccggc acacattcac
     7801 tactaaaaca atagctgttt gcgcttacct acagggcgca aaagccatat ttcgatgtga
     7861 aactcgtacc tcagccacgc tgtggataag tctgcggagc attccggtac aagcgccggc
     7921 ttatgcacag cggcaggcgt cacgcccggt ttgtccccat ttcgggtacg ccgtcggcgc
     7981 atacccgtcc ccatgcttca ggacagttca aaccattgat atgacaacga ataaacggct
     8041 tgtccacagg cagggccggc ctctactact accactagtt ttttataaga acatcaatga
     8101 acaaccaggg gcgaaatccg gccgaggccg ccatgtaaaa aaaaccgcca tcgtcggcgc
     8161 ttgattgggg caagtgaggg ggccgggctc agcgccacat ggcgtagccc acgccaatgg
     8221 ccgccaccaa gcccacggcg tcggccagca gggcgcaggc cagggcgtgg cggtggtgtt
     8281 tcacgcccac gctgccgaag tacacggcca gcacgtagaa ggtggtctcg gtggagccct
     8341 ggatgatggc tgccaggcgc ccgacgaagg agtccacgcc gtgcgtctgc agcacgtcga
     8401 tcatcaggcc gcgcgcaccc gcgcccgaga gcaccttcat cagccccacc ggcagggccg
     8461 gcaaaaaatc ggtgttccag cccaaagttg ccacgcccgc acctatggcc gagagcagcg
     8521 catccatgca accggcagcg cgaaacaccc caatggccac caggatggcg atcagatagg
     8581 gaatgatctg caccgccacg ccaaagcctt ccttggcgcc gtcgatgaag gcgtcgtaca
     8641 tgttcacgcg gcgccaggtg ccggccagca ggaacagaag tacgacgccc aggatcaccg
     8701 ccgcgcccgt ggtgcccgcc acctgggccg cgcgttcagc cggcagccgc gccaggccgg
     8761 ccacggctgc cgccagcagg ccgccgacgg ccagca
//
LOCUS       DAOMTQ010000127         8777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1680_length_8777_cov_27.295689, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000127 DAOMTQ010000000
VERSION     DAOMTQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1680_length_8777_cov_27.295689"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..731
                     /locus_tag="PK518_11740"
     CDS             84..731
                     /locus_tag="PK518_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49630.1"
                     /translation="MNTEAARLSAGEGVNTAPGLTLTGLYNVLAALREGRPLTPKEKT
                     IHAQGLVGVLRELHDQLDAAVLAAYGLPEGSSGDALLEPLVRLNAQRAQQEAQGQIHW
                     LRPDFQNPQETHQNQELPALVTQAQDPDFYDEKSLSKQEQPAPTPWPATLPEQVRAVA
                     DLLATSPAPLPLAAIEARFKGRGPWKRGLPTLLSTLEALGRAQCVPQDGASAWRA"
     gene            1028..1387
                     /locus_tag="PK518_11745"
     CDS             1028..1387
                     /locus_tag="PK518_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HRM49631.1"
                     /translation="MKNLDDYPFEIRPLAAAEGGGFLISFPDFSECISDGETIEEAIA
                     HGRVALAATMAALQAKGLPVPAPKSGGVASGKFVARVPKTIHAQLAARAKAEGVSLNT
                     LVLAFIAEGLGRREHLA"
     gene            1471..2493
                     /locus_tag="PK518_11750"
     CDS             1471..2493
                     /locus_tag="PK518_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRM49632.1"
                     /translation="MCFSRRPLPLLAALCGLATLFALPALAGSLHGDATPRPFSQPAP
                     LAQELRHKMEHPLVRELQVRLRHAKYLALYDVDDRFGLKTRETVRKFQKDHGLRATGI
                     VDQATWDKLLPKSHQPTAAELNNTDVGAWFTGPGQAGYIKELQHRLKQIGHYQGAVDG
                     QYQQATQQAIAGFRTQAGLPASTVMDERTWARLIAKTRNPRYAQLFDKPPTSQMTQEL
                     DTRCLTGKVVCISWEQKKMSLVQDGRTLFTRAARFARPGWESERGEFRIWYMNSDTVS
                     TIFGERTPMPYAIFYDKNVAIHYSDDFDQVGYEGGSHGCSQLNDYQTAKWLYEQVKVG
                     DKVVVY"
     gene            2513..3631
                     /gene="hemE"
                     /locus_tag="PK518_11755"
     CDS             2513..3631
                     /gene="hemE"
                     /locus_tag="PK518_11755"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRM49633.1"
                     /translation="MTTSFAPLANDTFLRACRRQATDYTPLWLMRQAGRYLPEYKATR
                     AKAGSFMGLATNVDYATEVTLQPLDRFALDAAILFSDILTVPDAMGLGLSFAEGEGPK
                     FAKTVRTEADVAELAVPDMDKLRYVFDAVTSIRRALNGRVPLIGFSGSPWTLACYMVE
                     GKGSDDYRLVKSLMYSRPDLMHRILAVNADAVAQYLNAQIDAGAQAVMIFDSWGGVLA
                     DGAFQQFSLEYTRRVLAQLKRTGVDGQDVPRICFTKGGGIWLQDMKALDCEVLGLDWT
                     ANLGQARAIVGGSAGGPGKALQGNIDPNVLFAPPEAVAREARAVLDSFGQPHTDRTTT
                     GPTHIFNLGHGISQHTPPEHVAALVETVHGYSRQLRQR"
     gene            4249..6453
                     /gene="priA"
                     /locus_tag="PK518_11760"
     CDS             4249..6453
                     /gene="priA"
                     /locus_tag="PK518_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRM49634.1"
                     /translation="MNLPAAAPAAHLLQVAVSTPAHSALGDLLSYACDRPLPPGTLVR
                     VPLGRREVLGVVWDAPAEPPPLPAGMAPRAVAGVLEGMAPLDAAWRRLVAFAARYYQR
                     ALGELALAALPPQLRELTPEQLARRMRRTSPAFPPLPQAGEGGGEGCKNGHNAQSPAH
                     PCPLPQGGEGVVGSISLSAEQASAAAQIDTNPGPFLLFGSTGSGKTEVYLHSVQQALA
                     ASPAAQALVMVPEINLTPQLEARFRERFAPLYGDAAVVSLHSGMTNPQRLKSWLAAHA
                     GAARIVLGTRMAIFASLPGLRLIVVDEEHDASYKQQEGARYSARDLALWRARDQGAKV
                     ILGSATPSLESWHASDPAVGRYQRLEMPSRIGAGALPRLRLIDMGQQPRGAIFAPPLL
                     AAITERVERGEQCLVLLNRRGFAPVLHCHACGWKSDCPHCSAHQVFHKIDRTLRCHHC
                     GFAQRVPRACPSCGNPDIAPVGRGTEQLEEQLAQLLRNVITPERRAARVARIDADTTK
                     AKGALEEQLAQVHAGDVDVLVGTQMVAKGHDFRRITLVAAVQPDGALFSSDFRAPERL
                     FALLMQAAGRAGRDARYVAAQESHPEMWLQTWHPDHALYAALRRHDYPAFAAQQLAER
                     AGAGMPPFAHQALVRADARSQETAQAFLQAAAEAARTAALPGLEQVFIFPPVPLAVQR
                     VAGVERAQMLLESPQRGALQRFLAAWQPLLHAERARHKGLVRWLVDVDPQGI"
     gene            complement(6463..6915)
                     /locus_tag="PK518_11765"
     CDS             complement(6463..6915)
                     /locus_tag="PK518_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM49635.1"
                     /translation="MIHDLHHTIAALAVKPPCALTLTFADGVTLPVDLAEVAHAYPAL
                     AALADPALFATARIDARGGYVVWIDDDLELAADNLRNMAVEQAGGIGSERLANWMHKH
                     GLTQERAADAIGVSRRMLNYYLSGAKAIPKTVWLACLGWEAMKESAPA"
     gene            complement(6912..7142)
                     /locus_tag="PK518_11770"
     CDS             complement(6912..7142)
                     /locus_tag="PK518_11770"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRM49636.1"
                     /translation="MPTLHKLPNKSIRVNVPDHLPPHLHVVMADRRDALVDLTTLAII
                     SRTLRASEIADALAWIEANRAYCVQVFKECNP"
     gene            7332..8456
                     /locus_tag="PK518_11775"
     CDS             7332..8456
                     /locus_tag="PK518_11775"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HRM49637.1"
                     /translation="MTFQALRMSLVATAIAVAGFAAAPTLALAQDASAAAAAAAKAAA
                     MAKPNVVVLATGGTIAGAGASAANSATYTAAKVPVDKLLAGLPELANVANVKGEQVFQ
                     IASESFANDNLLTLARRVSQLAKQPDVDGIVITHGTDTLAETAYFLSLTVHTDKPIAV
                     VGSMRPGTALSADGALNLLSAVSVAASKDARGKGVLITMNDQIQSARDTNKDVNIETR
                     AFQSQWGSLGMVVEGKNYWFRAPVKRHTTQSEFNIDDITSLPSVEIVYSYGNVQPTAV
                     QALAAAGAKAIIHAGTGNGSVANRMVEPLRQVQAQGVTIVRSSRVPYGFVLRNAEQPD
                     DKYDWVVAHDMRPEKARILTMLALAKGANTKELQRMFWEY"
BASE COUNT         1508 a         3061 c         2846 g         1362 t
ORIGIN      
        1 agggcagggg tgagggtgca ggcagcgggc gcagcgccag cacaccccct cacccccacc
       61 ctctccccca agggggcgag ggagtgaata ccgaagcagc gcgcctgtca gcgggcgagg
      121 gagtaaatac cgcgcccggc ctgaccctca ccggcctgta caacgtgctg gccgccctgc
      181 gtgagggccg ccccctgacg cccaaggaaa agaccatcca cgcccagggc ctggtagggg
      241 tgctgcgcga gctgcacgac cagctggacg ccgccgtgct ggccgcctac ggcctgcccg
      301 agggcagcag cggcgacgcc ctgctggagc cgctggtgcg cctgaacgcc cagcgcgccc
      361 agcaagaggc ccagggccag atccactggc tgcgcccaga ctttcagaat ccacaagaaa
      421 cacatcaaaa ccaggagctg ccagcgcttg ttacacaagc gcaagaccct gatttttatg
      481 atgaaaaatc actatcaaag caagagcaac cggcgccaac cccctggccc gccaccctgc
      541 ccgagcaggt gcgcgccgtg gctgacctgc tggccaccag ccctgcgccc ctgcccctgg
      601 cggcgataga ggcccgcttc aagggccgcg gcccctggaa gcgcggcctg cccacgctgc
      661 tgtccaccct ggaggccctg ggccgcgcgc aatgcgtgcc gcaagacggc gccagcgcct
      721 ggcgcgcctg accgggagcg ccggcgtccc gccggcatgg ttgatgcgca gtatcaccag
      781 gaatactatt caacccatga acaccgccgc aaagctgctt gaagccatgc accaccagcc
      841 gctggactgg cgcatcgaac agcttcagac ggtggcccgg caacatggaa ttgactagcg
      901 gcacgatggc ggcagccatt gcgtgtttgt gcgcgcagac ggcacaaccc tgccggtgcc
      961 cgcgcaccgc ccgatcaagc ccatctatgt gaagaaattt ctggccctgg tcagcggagc
     1021 gtgagcgatg aaaaacctgg acgactaccc gtttgagatt cgacccctgg cggcggccga
     1081 gggcggcggt ttcctgatct cttttccgga tttttcagaa tgcatctcgg acggcgagac
     1141 catagaagaa gccatcgccc atggccgcgt cgcactggcc gccaccatgg ccgcgctgca
     1201 ggccaagggc ctgccggtgc ccgcgcccaa gagcggcggc gtggcctcgg gcaagtttgt
     1261 cgcccgcgtg cccaagacaa tccatgcgca gctggcggcc agggccaagg ccgagggcgt
     1321 ttcactcaac accctggtgc tggccttcat cgccgagggc ctggggcggc gcgagcacct
     1381 ggcctgacag ggagcgccgg catggacacg ctgcacctct tgagcgcgct caaccatccc
     1441 cggcgccagg cttcgggata atcccgcccc atgtgttttt cgcgccgtcc ccttcccctg
     1501 ctcgccgccc tgtgcggcct ggccaccctg tttgccttgc ccgccctggc cggctccctg
     1561 catggcgacg ccacgccacg ccccttcagc cagccggcgc cgctggcgca ggagctgcgc
     1621 cacaagatgg agcaccccct ggtgcgcgag ctgcaggtgc ggttgcgcca tgccaagtac
     1681 ctggcgctgt atgacgtgga cgatcgcttc ggcctgaaga cgcgcgagac ggtgcgcaag
     1741 tttcagaagg atcacggcct gcgcgccacc ggcatcgtgg accaggccac ctgggacaag
     1801 ctgctgccca agtcgcacca gcccacggcc gccgagctga acaacaccga cgtgggcgcc
     1861 tggttcaccg gccccgggca ggcgggctac atcaaggagc tgcagcaccg cctgaagcag
     1921 ataggccatt accagggcgc cgtggacggc cagtaccagc aggccacgca gcaggcgatt
     1981 gccggctttc gcacccaggc cggcctgccg gccagcacag tgatggacga gcgcacctgg
     2041 gcgcgcctga tcgccaagac gcgcaacccg cgctacgccc agctgttcga caagccgcct
     2101 accagccaga tgacgcagga gctggacacg cgctgcctga cgggcaaggt ggtgtgcatc
     2161 tcctgggagc aaaagaagat gtcgctggtg caggacggcc gcacgctgtt cacgcgcgcg
     2221 gcgcgctttg cccggccggg ctgggagagc gagcgcggcg agttccgcat ctggtacatg
     2281 aacagcgaca cggtctcgac catctttggc gagcgcacgc ccatgccgta tgccatcttt
     2341 tatgacaaga acgtggccat ccattactcg gacgatttcg accaggtggg ctacgagggc
     2401 ggctcgcacg gctgcagcca gctgaacgac taccagaccg ccaaatggct gtatgagcag
     2461 gtgaaggtgg gtgacaaggt ggtggtgtat tgaggctgcg acaatcggcc ccatgactac
     2521 ctcctttgcc cccctcgcca acgacacttt tttgcgcgcc tgccgccgcc aggccaccga
     2581 ctacacgccc ctgtggctga tgcgccaggc cggccgctac ctgcccgagt acaaggccac
     2641 gcgcgccaag gccggcagct tcatgggcct ggccaccaac gtggattacg ccaccgaggt
     2701 gacgctgcag ccgctggatc gctttgcgct ggatgcggcg attttgttca gcgacatcct
     2761 gaccgtgccc gacgccatgg gcctgggcct gtcctttgcc gagggcgagg ggccgaagtt
     2821 cgccaagacc gtgcgcaccg aggccgacgt ggccgagctg gccgtgcccg acatggacaa
     2881 gctgcgctat gtgttcgacg ccgtcaccag catccgccgc gccctgaacg gccgcgtgcc
     2941 gctgatcggc ttctcgggca gcccctggac gctggcctgc tacatggtcg agggcaaggg
     3001 atcggacgac taccgcctgg tcaagagcct gatgtacagc cgcccggacc tgatgcaccg
     3061 catcctggcc gtgaacgccg acgccgtggc gcagtatctg aacgcgcaga ttgacgccgg
     3121 cgcccaggcg gtgatgattt ttgacagctg gggcggcgtg ctggccgacg gcgccttcca
     3181 gcagttcagc ctggaataca cccgccgcgt gctggcgcaa ttgaagcgca ccggcgtgga
     3241 cggccaggac gtgccgcgca tctgcttcac caagggcggc ggcatctggc tgcaggacat
     3301 gaaggcgctc gactgcgagg tgctgggcct ggattggacg gccaacctgg gccaggcgcg
     3361 cgccatcgtg ggcggcagcg caggcggccc gggcaaggcg ctgcagggca atatcgaccc
     3421 caacgtgctg tttgccccac ccgaggccgt ggcgcgcgag gcacgcgccg tgctcgacag
     3481 ctttggccag ccgcacactg accgcacgac gaccgggccg acgcatatct tcaacctggg
     3541 ccacggcatc agccagcaca ccccgcccga gcatgtggcg gccctggtgg agacggtgca
     3601 cggctactcg cgccagctgc gtcagcggtg aatacccact cacattgacg cgcatgaggc
     3661 tatgacttat gcacatttac gccggggtgg ccatggggcg acaaagcccc acgcagcccc
     3721 cggcgcaaca gcgtcacaaa aatgaaacag gcgcgcaagt tattgattta tttaaacaag
     3781 ccggccatgc cgcttttctg ggcaggctaa acaaagcccg ctgcgacaag ggcttgcggg
     3841 cgccgggcca tggatatcaa caatgttatc cacagaaatt ctggacttcg cctgaatacc
     3901 ctgccaaatc aagcacttgc ggcccaatgt gcaagaaaga tcgagtgcag cggcatctgc
     3961 ctagcatatt ttgcggccac atccggctat tgacagcgca gttgtgcaca tgtttggcgc
     4021 agcggtgcct ggccagggcc aagcgccggc aattgccggg ccacgccttg gggcatgaca
     4081 aagatcattg attcatatgg ggttttcttt attcgcggct gcaaatggac tagcacccgg
     4141 gcacgcagcc atgcggcctg gcgggctggt cacacgggct ttcaacaaag ttatccacag
     4201 acccccgatt gactgtgggc aaggcggcgc cagccgcgcg ctgctgccat gaacctgccc
     4261 gctgcagcgc ccgccgcgca tctgctgcag gtggcggtga gcacgcccgc gcacagcgcc
     4321 ctgggcgact tgctgagcta tgcctgcgac cgcccgctgc cccccggcac cctggtgcgc
     4381 gtgccactgg gccggcgcga ggtgctgggc gtggtctggg acgcacccgc cgaacccccg
     4441 cccctccccg ccggcatggc gccgcgcgcc gtcgccgggg tgctggaggg gatggcccca
     4501 ctggacgccg cctggcgccg cctggtggcc tttgccgcgc gctactacca gcgtgccctg
     4561 ggcgagctgg ccctggccgc cctgccgccg cagctgcgcg agctgacgcc cgagcagctg
     4621 gcgcggcgca tgcgccgcac cagtcctgcc ttcccccctc tcccgcaggc gggagagggg
     4681 gggggggagg gttgcaaaaa tgggcacaac gcccaatcac ccgctcaccc ctgccctctc
     4741 ccccaagggg gcgagggagt tgtcggctca atcagcctga gcgcagaaca ggcaagcgcc
     4801 gcagcccaaa ttgacaccaa tcccggcccc tttctgctgt tcggcagcac cggcagcggc
     4861 aagaccgagg tctatctgca cagcgtgcag caggcgctgg ccgccagccc agccgcccag
     4921 gcgctggtga tggtgcccga gatcaacctg acgccccagc tggaggcgcg ctttcgcgag
     4981 cgctttgcgc cgctgtatgg cgacgcggcc gtggtcagcc tgcacagcgg catgaccaac
     5041 ccgcagcgcc tgaagagttg gctggcggcg cacgccggcg ccgcgcgcat cgtgctgggc
     5101 acgcgcatgg ccatttttgc cagcctgccg gggctacggc tcatcgtggt cgatgaggag
     5161 catgacgcca gctacaagca gcaggagggc gcgcgctact cggcgcgcga cctggcgctg
     5221 tggcgcgcgc gcgaccaggg cgccaaggtc atcctgggca gcgccacgcc ctcgctggag
     5281 agctggcatg cgagcgaccc ggccgtgggc cgctaccagc gcctggagat gcccagccgc
     5341 ataggcgcgg gcgcgctgcc gcgcctgcgc ctgatcgaca tgggccagca gccgcgcggc
     5401 gcaatatttg ccccgccgct gctggcggcg attaccgagc gcgtggagcg cggcgagcaa
     5461 tgcctggtgc tgctcaaccg ccgcggcttc gccccggtgc tgcactgcca cgcctgcggc
     5521 tggaagagcg actgcccaca ttgcagcgcg caccaggtgt tccacaagat agaccgcacg
     5581 ctgcgctgcc accactgcgg ctttgcccag cgcgtgccgc gcgcctgccc gtcctgtggc
     5641 aaccccgaca tcgcccccgt gggcaggggc acggagcagc tcgaagagca gctggcgcaa
     5701 ttgctgcgca acgtcatcac ccccgagcgc cgcgccgccc gcgtggcgcg catagacgcc
     5761 gacacgacga aggccaaggg cgcgctggag gagcagctgg cccaggtgca cgccggcgat
     5821 gtggacgtgc tggtgggcac gcagatggtg gccaaggggc acgactttcg ccgcatcacc
     5881 ctggtggccg ccgtgcagcc cgacggggcg ctgttttcca gcgactttcg cgcgccggag
     5941 cggctgtttg ccctgctgat gcaggccgcc ggccgcgccg ggcgcgacgc gcgctatgtg
     6001 gccgcgcagg agagccaccc cgagatgtgg ctgcagacct ggcacccgga tcacgccctc
     6061 tacgccgccc tgcgccgcca cgactacccg gccttcgccg cgcaacagct ggccgagcgc
     6121 gcgggcgccg gcatgccgcc ctttgcccac caggccctgg tgcgcgccga cgcgcgcagc
     6181 caggagactg cccaggcctt cctgcaggcc gcagccgagg ccgcacgcac cgccgccctg
     6241 ccggggctgg agcaggtgtt cattttcccg cccgtgcccc tggccgtgca gcgcgtggcg
     6301 ggcgtggaac gggcgcaaat gctgctggag agcccccagc gcggcgcgct acagcgcttt
     6361 ctggccgcct ggcagcccct gctgcatgcc gagcgcgcgc ggcacaaggg gctggtgcgc
     6421 tggctggtgg atgtggaccc gcaggggatt tgagggtgag cctcaggcgg gcgccgactc
     6481 cttcatcgcc tcccagccca ggcaggccag ccagacggtc ttgggtattg ccttcgcacc
     6541 cgacaggtag tagttcagca tgcggcgcga tacgccaatg gcatcggcgg cgcgctcctg
     6601 cgtcaggccg tgcttgtgca tccagttggc aaggcgttcg cttccaatgc cgccggcctg
     6661 ttcgaccgcc atgttgcgca ggttgtcggc cgccagttca aggtcatcgt caatccacac
     6721 cacatatcca ccccgcgcat cgatgcgcgc cgtggcaaac aacgccgggt cggcaagggc
     6781 cgccagcgcc gggtaggcat gggccacctc agccaggtcg acgggcagcg tcacgccatc
     6841 tgcaaatgtc agcgtcaggg cgcaaggcgg cttcactgcc agggcggcga tggtgtgatg
     6901 gagatcgtga atcatgggtt gcactccttg aaaacttgca cgcaatacgc ccggtttgcc
     6961 tctatccacg ccagtgcatc ggcaatttca gaggcccgca acgtgcggct gatgattgcc
     7021 agcgtcgtca aatccaccag cgcatcacgc ctgtccgcca tcaccacatg cagatggggc
     7081 ggcaagtggt ctggcacgtt gacgcggata ctcttgtttg gcaacttgtg aagcgttggc
     7141 atgcatgtat tttagtgcaa ccattgcaca aacatcaacg gaatgagccc ccaggaccaa
     7201 gcaaggccgc tgtgccaata ccgctcctgg cgcagcggtt ttgccgggtt tccaccattg
     7261 gcaactgcgc gaccatgcga cacagtgcag ccccggttca caagaccgct tttttgttta
     7321 tggattccca catgaccttc caagccttgc gcatgtccct ggtggccacc gccatcgccg
     7381 ttgccggttt tgccgccgcc cccaccctcg cactggcgca ggacgccagc gccgcggccg
     7441 cggcagcagc caaggccgca gccatggcca agccgaatgt ggtggtgctg gccaccggcg
     7501 gcaccattgc cggcgcaggc gcatcagcgg ccaacagcgc cacctacacg gcagccaagg
     7561 tgccggtgga caagctgctg gccggcctgc cggagctggc gaatgtcgcc aacgtcaagg
     7621 gcgaacaggt gttccagatc gcctcggaaa gctttgccaa cgacaacctg ctgaccctgg
     7681 ccaggcgcgt ctcgcagctg gccaagcagc ctgacgtgga cggcatcgtc atcacccacg
     7741 gcaccgacac cctggccgaa acggcgtact tcctgagcct gacggtgcac accgacaagc
     7801 ccatcgccgt ggtcggctcg atgcgcccgg gcactgccct gtcggccgac ggcgcgctca
     7861 acctgctgag cgccgtgagc gtggccgcgt ccaaggacgc caggggcaag ggcgtgctga
     7921 tcaccatgaa cgaccagatc cagagcgcgc gcgacaccaa caaggacgtg aacatcgaga
     7981 cccgcgcctt ccagagccag tggggctcgc tgggcatggt ggtcgagggc aagaactact
     8041 ggttccgcgc cccggtcaag cgccacacca cgcagtccga gttcaacatc gacgacatca
     8101 ccagcctgcc ctcggtggag atcgtctact cctacggcaa cgtgcagccc accgccgtgc
     8161 aggccctggc cgccgccggc gccaaggcca tcatccacgc cggcacgggc aatggctcgg
     8221 tcgccaaccg catggtcgag ccgctgcgcc aggtgcaggc ccagggcgtg accatcgtgc
     8281 gctcctcgcg cgtgccctac ggcttcgtgc tgcgcaacgc cgagcagccc gacgacaagt
     8341 acgactgggt ggtggcgcac gacatgcgcc ccgagaaggc acgcatcctg accatgctgg
     8401 ccctggccaa gggcgccaat accaaggaac tgcagcgcat gttctgggag tattgagact
     8461 cccgggcata tgtcgtggcc tttgctcatg gtattgatca aggtgttcgc agccggcctg
     8521 ctcgccctgt ttggcgcctg gtgatgggcc gtggcgctgg cgctgatctg ggtgctgcct
     8581 tggctggctg ttgtaatttt ggcctccagc gcttggtggt attgcgcttg tagctcttct
     8641 ttttgatgtt gctggccggg atgtgcgccc ggcggcgcac ctacctttct ttgcttcgtc
     8701 aaagaaaggt aggccaaaga aagacgaccc tactgtccgt gtcccttcgc ttcgctacgg
     8761 gcaacctgcg atgctcg
//
LOCUS       DAOMTQ010000128         8409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1846_length_8409_cov_28.965166, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000128 DAOMTQ010000000
VERSION     DAOMTQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1846_length_8409_cov_28.965166"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PK518_11780"
     CDS             complement(<1..902)
                     /locus_tag="PK518_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM49638.1"
                     /translation="MKVAVIGSGISGLAAAHRLRGQARVTLFEAGRYFGGHTHTVDVS
                     LPDAHGQTVTHGVDTGFLVFNERTYPGLIALLGELQVPTAASDMSFSVQVPGAGALGA
                     GALEWSGSSLATVFAQRRNLLRPRFLGMLSELLRFNRLCTALADSGQEQALAQPLGEF
                     LDQHGFGAAFRHWYFLPMLGCIWSCPTDQMLRFPVATMVRFCHNHGLIQVQNRPQWHT
                     VAGGARQYVHAILDGLDARLQTPVERIERNAAGVFIRTGSGVEHFDAVVLAVHSDQAL
                     RLLARPSAAEQRVLGAIRYQPNRAV"
     gene            1021..1995
                     /locus_tag="PK518_11785"
     CDS             1021..1995
                     /locus_tag="PK518_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230323.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRM49639.1"
                     /translation="MNSLTPPTVTAPPAPGNAGLPIAAVERETGLGKDTLRVWERRYR
                     FPTPSRDSSGDRLYSLEQVQQLKLISRLLDSGLRPGKVVGLGQAALQDLLTQHAPVSA
                     VILSKTIMDNAGADPLIEALLHAIGTHDPRALRHGLSHAQLRMGLAPFVTDLVAPLTT
                     AVGEAWAQGRFEVFEEHLYTEVITGVLRHAIASLTPQPVPQGPKVLLTTVPQEQHSLG
                     LLMVEALLALEGCTCVSLGTQTPLTDIAQAALAHRADVVALSFSNLHKAAVVQTSLRE
                     LRAQLPATTALWVGGACTALHQWPLPGISAVQHLSGLQPLVAQWRHAH"
     gene            2014..2616
                     /locus_tag="PK518_11790"
     CDS             2014..2616
                     /locus_tag="PK518_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRM49640.1"
                     /translation="MQFIARTLASARTPLSRRQRLGLLALLVVGTGLLLAGCASTRPP
                     PGVTAVTPFDVQRYAGRWYELARLDHAFERGMTDVTATYTAQADGSVRVVNRGYAPAT
                     SQWREAVGKALFTGAPTTGSLKVSFFGPFYGGYHVAALDPDYRWALVLGPDTSYCWIL
                     ARDKQLPAAQRDAIVARAQALGVDTSSLIWVPHERQDPAH"
     gene            2630..4177
                     /locus_tag="PK518_11795"
     CDS             2630..4177
                     /locus_tag="PK518_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HRM49641.1"
                     /translation="MGYTVVWFKRDLRVHDHAPLAHAAAHGPVLCLYVLEPSLWTQPD
                     SALQHYQFLRESLRDLAQQLRSCGARLQLAVGEVPEVLARLHALQPFARLVSHEETGN
                     GATYARDLAVARWCRRQGVAWQEWPQHGVVRRLPSREQWHGLWQAHMQAPCLPPPLPA
                     SLRSVCLPWRDTWPAPETMGLSDAHDPPQRQRGGRAPGQQVLHDFLHARAAFYRGGIS
                     SPLTAPTACSRLSPYLALGCLGMREVVQATQQRQVQLKLQDAQQAAHVRQGLAAFMSR
                     MHWHCHFIQKLESEPELEWRNLHRGYDGLREDDWNPAHFDALQAGRTGWPMVDACVAM
                     LRETGWVNFRMRAMLVSVAAYPLWLHWRAVGLWLARQFLDYEPGIHWSQMQMQAGTTG
                     INTTRVYNPIKQAQDHDPHGVFVRRWLPALRRVPDAWLFEPWRMPPEVQARCGVRVGQ
                     DIATPLVDLASATKAAKARLHALRNQEPIRAAKAAIVEKHGSRLVRHTAVRRQLPFTS
                     PQQSLDF"
     gene            4181..4345
                     /locus_tag="PK518_11800"
     CDS             4181..4345
                     /locus_tag="PK518_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HRM49642.1"
                     /translation="MHRKPHLPQKHCLCCGRPFAWRKKWAKVWDEVKFCSERCRRQRK
                     APHPPSGLQE"
     gene            4342..5880
                     /locus_tag="PK518_11805"
     CDS             4342..5880
                     /locus_tag="PK518_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRM49643.1"
                     /translation="MTAAHTLRLVLGDQLHPGHSWFSQVDEQVVYVLMEVRQETDYVL
                     HHAQKILAIFAAMRDFARYLRAGGHRVRYVALDDPGNRQSIPGNLAALAAHYAAQRIE
                     WQQPDEWRLDAQLQAWGAAQPLDWAVMDSEHFLTQRGELAALMGQRKQWLMEHFYRHM
                     RRRYKLLLDADGAPEGGQWNFDHDNRKRWPGTPPVPADWRPQHDHSDLWRMVQRAGVQ
                     SFGDPQAAALRWPLNRTEALACLDAFITTALPHFGDYEDALAAQAPRLFHSLLSFALN
                     VKMLHPLEVVQRAKAAWRGGQAPLAAVEGFVRQIVGWREYVRGIYWANMPGYETRNAL
                     GHHAPLPRWFWNGETHMRCLHLAITQSLQTAHAHHIQRLMVIGNFALLAGLEPQALHR
                     WYLGIYIDAFEWVELPNTLGMSQRADGGVIATKPYVSSAAYLQRMGDYCQGCAYDPKQ
                     KTGARACPFNALYWDFFERHAPTLQGNPRLALVYRQLQKMSDAQRAALRTQAGHLRER
                     LDTL"
     gene            5905..6603
                     /locus_tag="PK518_11810"
     CDS             5905..6603
                     /locus_tag="PK518_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM49644.1"
                     /translation="MQSLPAGYRALVLGASGAIGGALAAQLRDDPYCAKMLTLGRSTT
                     PAVDFDAPPSIAEAAHSLFTQGPWNLVLVATGMLQGPTGKPEKRLTDLSAEHMAASFA
                     VNTIGPALALAHFAPQLAHGERSVLAVLSAKVGSIGDNRLGGWYSYRASKAALNMVVK
                     TAAIELARTHPQAVVAALHPGTVDSALSAPFRGAQIWRPARDAARDLLAVIDGLQPED
                     SGGFWAYDGQRLRW"
     gene            6633..7535
                     /locus_tag="PK518_11815"
     CDS             6633..7535
                     /locus_tag="PK518_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRM49645.1"
                     /translation="MRILLTGGTGLIGRALCQHWQRQGHDLFVWSRDTAQVPRLCSGA
                     QGIAQLQALDGSAPLDAVVNLAGAPIADRPWTAARRDLLWHSRVDLTRTLVDWMGRQA
                     APPRVLLSGSAVGWYGDRGEQWLAEDSAPGNADFGSRLCVAWEQEAERARQWGVRVAL
                     LRTAPVLAPQGGMLARLLPPFRLGLGGRLGSGQQWMPWIHLDDQVALMDHLLHNDACS
                     GPYNACAPEAVRNTEFTQTLARTLGRPAVLPAPAWVLRLALGEMSVLLLGGQRLAPRR
                     TQGAGFAWRHPQLAAALAQLLRKS"
     gene            complement(7615..8214)
                     /locus_tag="PK518_11820"
     CDS             complement(7615..8214)
                     /locus_tag="PK518_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRM49646.1"
                     /translation="MSGRALAFLLVISHPMAFAGVPSVPPAPTAAAAGEQALSGCPAV
                     LRHRFPRLQDDSPQSLCQYQGKVILVVNTASYCGFTYQYQGLEALFQKYKDRGLVVLG
                     FPSNDFFQEKNDNKEIADFCYNTYGVIFPMFARSAVRGSDANPLYKQLAQMTGKVPGW
                     NFNKYLIDRSGLSVTHYPSTVEPESTAFLGQLEKLLAKP"
BASE COUNT         1376 a         2891 c         2784 g         1358 t
ORIGIN      
        1 acggcgcggt tgggctggta gcggatggcg cccagcaccc gctgctcggc ggcgctgggc
       61 cgcgccagca ggcgcaaggc ctggtcgctg tgtacggcca gcaccacggc atcgaagtgc
      121 tccacgccgc tgccagtgcg gatgaataca ccagcggcat tgcgctcgat gcgctctaca
      181 ggggtttgca gccgcgcatc caggccgtcc aggatggcat gcacatactg ccgcgccccg
      241 cccgccacgg tgtgccactg cggccggttc tgcacctgga tcaggccgtg gttgtggcaa
      301 aagcgcacca tggtggccac gggaaagcgc agcatctggt cggtcgggca gctccagata
      361 cagcccagca tgggcaggaa ataccagtgg cgaaacgccg cgccaaagcc atgctggtcc
      421 aaaaactcgc ccagcggctg cgccagcgcc tgttcctggc cgctgtcggc cagcgcggtg
      481 cacaggcggt tgaagcgcag cagctccgac agcatgccca aaaagcgcgg gcgcagcagg
      541 ttgcgacgct gggcaaacac ggtggccagg ctggagccgc tccactccag cgccccggcg
      601 cccagcgcgc ccgcgcccgg tacctggacg gaaaacgaca tgtccgacgc ggccgtgggc
      661 acctgcaatt cgcccagcag cgcgatcagg ccggggtagg tgcgctcgtt gaacaccaga
      721 aagccggtgt ccaccccgtg cgtcacggtc tggccatgcg catcgggcaa ggacacatcc
      781 acggtgtggg tgtggccgcc gaagtagcga cccgcctcga acagcgtgac ccgcgcctgc
      841 ccgcgcagcc ggtgtgcggc cgccaggccc gaaatgcccg atccaatgac tgcgactttc
      901 atggactttt ccgatccgtg atgcttgccc gtgcagcacg gttgcgctgc tcctgtggcg
      961 taatgtacta gacaaaaaca tttttgtcct agacaattaa acgaacaagc cccaatcgcc
     1021 atgaattcac tgacccctcc gaccgtcacg gcaccgcccg cgcccggcaa tgccgggctg
     1081 ccgattgccg ctgtcgaacg cgaaaccggc ctgggcaagg acacgctgcg cgtgtgggag
     1141 cggcgctatc ggtttcccac gccgtcgcgt gattcctcgg gcgacaggct gtacagcctg
     1201 gagcaggtgc agcagctcaa gctgatcagt cggctgctcg attccggctt gcgccctggc
     1261 aaggtggtcg gcctgggtca ggctgctttg caagacctgt tgacgcagca cgccccggta
     1321 tcggctgtca ttttgtccaa gacaatcatg gacaatgcag gcgctgatcc cctgattgag
     1381 gcgctgcttc acgccattgg cacgcacgac ccgcgcgccc tgcggcacgg cctcagccat
     1441 gcccagttgc gcatggggct ggccccgttc gtgaccgatc tggtggcgcc gctgacgacg
     1501 gcggtgggcg aagcctgggc gcaggggcgt tttgaagtct ttgaagaaca tctgtacacc
     1561 gaggtcatca ccggcgtgtt gcgccatgcc atcgcctcgc tgaccccgca gcccgtgcca
     1621 caaggcccca aggtgctgct caccaccgtg ccgcaagagc agcacagcct gggactgctg
     1681 atggtcgagg cgctgttggc gctggagggc tgcacctgtg tgtcgctggg cacgcagacc
     1741 ccgttgaccg acatcgccca ggcggcgctg gcgcatcgcg ccgatgtggt ggcgctgagc
     1801 ttcagcaacc tgcacaaggc ggccgtggtg cagaccagcc tgcgcgagct gcgcgcccaa
     1861 ctgccggcca ccaccgcact gtgggtgggc ggcgcgtgta ccgccctaca ccaatggccg
     1921 ctgcccggca tcagcgccgt acagcatctt tcgggcttgc agcccttggt ggcgcagtgg
     1981 cgccacgccc attgaccgaa ggaaggattg gccatgcagt tcattgcacg cacgttagcc
     2041 agcgcacgca cccccctgtc gcgccgccaa cgcctcgggc tgctggccct gctggtggtc
     2101 ggcactggcc tgctgctggc cggctgcgcc agcacgcgcc cgccgcccgg cgtcacggcc
     2161 gtcacccctt ttgacgtgca gcgctacgcg ggccgctggt acgaactggc gcggctcgat
     2221 cacgcgttcg agcgcggtat gaccgatgtc actgccacct acaccgcgca ggctgacggc
     2281 agcgtgcgcg tggtcaaccg cggctatgcg cccgccacca gccagtggcg cgaggccgtg
     2341 ggcaaggcct tgttcaccgg cgcgcccacc acgggctccc tcaaggtatc cttcttcggg
     2401 ccgttctacg gcggctacca cgtcgccgcg ctggacccgg actaccgctg ggcgttggtg
     2461 ctggggccgg acaccagcta ctgctggatt ctggcgcgcg acaagcaact gcccgccgcg
     2521 cagcgcgacg ccatcgtggc gcgggcgcag gcgctgggcg tcgatacctc gtcgctgatc
     2581 tgggtaccgc acgagcgcca ggatccggcg cattgagcgg atgtgagtca tgggctacac
     2641 cgtcgtctgg ttcaagcgcg acttgcgggt acacgaccat gccccgctgg cacatgccgc
     2701 cgcgcatggc ccggtgctgt gcctgtacgt gctagagccc agcctgtgga cgcagccaga
     2761 cagtgcgctg cagcactacc agtttctgcg cgagagcctg cgcgacctgg cgcagcaatt
     2821 gcgcagttgc ggcgcgcggc tgcaactggc cgtgggcgag gtgcccgagg tgctggcccg
     2881 gctgcacgcc ctgcagccct ttgcccgcct ggtatcgcac gaggaaaccg gcaacggcgc
     2941 cacctacgcc cgcgacctgg ccgtggcccg ctggtgccgc cggcagggcg tggcctggca
     3001 ggaatggccg cagcacgggg tggtgcggcg cctgccctcg cgtgagcagt ggcacggcct
     3061 gtggcaggcg catatgcagg caccctgcct gccgccgccg ctgccggcga gcctgcgatc
     3121 cgtttgcttg ccgtggcgcg acacctggcc cgcgccggag accatggggc tgtctgacgc
     3181 ccacgacccg ccccagcgcc agcgcggcgg gcgcgcgccg ggccagcagg tgctgcatga
     3241 ttttctgcac gcgcgcgcag ccttttaccg cggcggcatc tcgtcgccgc tgacggcgcc
     3301 cacggcttgc tcgcgcctgt cgccctatct ggcgctgggc tgcctgggca tgcgcgaagt
     3361 ggtgcaggcc acgcagcaac ggcaagtgca gctcaagttg caggacgcac agcaagctgc
     3421 gcatgtgcgc cagggcttgg cggccttcat gagccggatg cactggcact gccacttcat
     3481 tcagaagctt gaatccgagc ccgagctgga gtggcgcaac ctgcaccgtg gctacgacgg
     3541 cctgcgtgaa gacgactgga accccgccca cttcgatgcc ctgcaggcgg gccgcactgg
     3601 ctggcccatg gtggacgcct gcgtggccat gctgcgcgag accggctggg tcaactttcg
     3661 catgcgcgcc atgctggtgt cggtggctgc ctacccgctg tggctgcatt ggcgcgccgt
     3721 ggggctgtgg ctggcgcggc agtttctgga ctacgagccc ggcattcact ggagccagat
     3781 gcagatgcag gccggcacca ccggcatcaa caccacgcgc gtctacaacc ccatcaagca
     3841 ggcgcaggat cacgaccccc atggtgtgtt cgtgcgccgc tggctgcccg cgctgcggcg
     3901 cgtgcccgat gcctggctgt tcgagccctg gcgcatgccg cccgaggtgc aagcgcgctg
     3961 tggcgtacgc gttgggcagg acattgcgac gccattggtc gatctggcca gcgccaccaa
     4021 ggccgccaag gcgcgcttac acgccttgcg caaccaggaa ccgatacgtg cggccaaagc
     4081 agcgatcgtg gaaaaacacg gctcgcgcct ggtgcgccac accgccgtcc gccgccagct
     4141 gccgttcacc tccccccagc aaagcctgga tttttgagcc atgcaccgca agccgcatct
     4201 gccgcaaaag cactgcctgt gctgcgggcg cccctttgca tggcgcaaaa aatgggccaa
     4261 ggtgtgggac gaggtgaagt tctgctccga gcgctgtcgc cgccagcgca aggccccgca
     4321 tccgccatcg gggttgcagg aatgacggcc gcccacaccc tgcgcttggt gctgggcgac
     4381 caactgcacc caggccacag ctggttttcc caggtggacg agcaagtggt gtatgtgctg
     4441 atggaggtgc gccaggagac cgactacgta ctgcaccacg cgcagaaaat cctcgccatc
     4501 ttcgccgcca tgcgcgactt tgcgcgctac ttgcgcgcgg gcggccaccg cgtgcgctac
     4561 gtcgcattgg acgaccccgg caaccgccag tccatccccg gcaacctggc agcactggcc
     4621 gcgcactacg ccgcccagcg catcgagtgg cagcagcccg acgaatggcg gctggacgcc
     4681 caactgcagg cctggggcgc cgcccagccg ctggattggg cggtgatgga cagcgagcat
     4741 tttttgaccc aacgcggcga gcttgccgca ctgatgggcc agcgtaagca gtggctgatg
     4801 gaacatttct accgccacat gcgccgccgc tacaagctgt tgttggacgc cgacggcgcc
     4861 cccgaaggcg ggcaatggaa ttttgaccac gacaaccgca aacgctggcc cggcaccccg
     4921 cccgtgcccg ccgactggcg cccccagcat gaccacagcg acttgtggcg catggtgcag
     4981 cgcgccgggg tgcaaagctt tggcgacccg caagccgccg ccctgcgctg gccgctgaac
     5041 cgcacagaag cgctggcctg cctggatgcc ttcatcacca cagcgctgcc gcattttggc
     5101 gactatgagg atgcactggc cgcccaggcg ccgcgcctgt tccattcgct gctgtcgttt
     5161 gcgctcaatg tgaagatgct gcacccgctg gaagtggtgc agcgcgccaa agccgcctgg
     5221 cgcgggggcc aggcgccgct ggctgccgta gaaggatttg tgcgccagat cgtgggctgg
     5281 cgcgaatacg tgcgcggcat ctactgggcc aacatgcccg gctacgagac gcgcaacgcc
     5341 ctcggtcatc acgccccgct gccgcgctgg ttctggaacg gcgagacgca catgcgctgc
     5401 ctgcaccttg ccatcacgca gtcgctgcaa acggcgcacg cgcaccacat ccagcggctg
     5461 atggtgatcg gcaacttcgc gctgcttgcc gggctggagc cgcaggcgct gcaccggtgg
     5521 tacctgggta tctacatcga cgccttcgag tgggtggagc tgcccaacac cctgggcatg
     5581 agccagcgcg ccgatggcgg cgtgattgca accaagccct atgtgagcag cgccgcctat
     5641 ctgcagcgca tgggcgacta ctgccagggc tgcgcctacg acccaaagca aaaaaccggc
     5701 gcgcgcgcct gccccttcaa cgccctgtac tgggattttt ttgagcgtca cgccccgacg
     5761 ttgcaaggca acccgcgcct ggcgctggtg taccgccagc tgcaaaaaat gtccgacgcg
     5821 cagcgcgcgg cgctgcgaac gcaagccggg catctgcgcg aacggctgga cacgctctga
     5881 ctacagcacc cgaaaggact ctccatgcaa tccctacccg ctggttaccg cgcgctggtg
     5941 cttggcgcct caggcgctat cggtggtgca ctggccgccc aattgcgaga cgacccatac
     6001 tgcgcaaaga tgctcacgtt ggggcgctcc accacgccag ctgtggactt cgacgccccg
     6061 cccagcattg ccgaggcggc gcactcattg tttacacaag gcccatggaa cctggtgctg
     6121 gtggccacag gcatgctgca aggtcccacg ggcaagcccg aaaagcgtct gacagacctg
     6181 agtgccgagc acatggcggc tagctttgcc gtcaacacca ttgggccagc cttggcgctg
     6241 gcgcatttcg ccccgcagtt ggcgcacggc gagcgcagcg tgctcgcggt gctttcagcc
     6301 aaagtcggca gcattggcga caaccgcctg ggtggttggt acagctaccg cgcctccaag
     6361 gcggcgctga acatggtggt aaagacggcg gccatcgaac tggcccgcac ccacccgcag
     6421 gcggtggtcg cagcgctgca cccgggcacc gtggactcgg cgctctcggc cccgtttcgc
     6481 ggcgcgcaaa tctggcggcc tgcccgcgac gctgcgcgcg acctcttggc ggtgatcgat
     6541 ggcttgcagc ccgaggacag cgggggcttc tgggcctatg acggccagcg cctgcgttgg
     6601 taacccgcac aacgattgga aggaaacgca ccatgcgcat tctgctcacc ggcggcaccg
     6661 gcctgatcgg ccgggccttg tgccagcact ggcagcgaca gggccacgac cttttcgtct
     6721 ggagccgtga cacggctcaa gtaccgcgcc tgtgcagcgg cgcacagggc atcgcgcaac
     6781 tgcaagcgct tgacggtagc gcgccgctgg acgccgtggt caatctcgca ggcgcgccga
     6841 ttgccgaccg cccctggacc gctgcgcggc gcgacctgct gtggcacagc cgtgtggatc
     6901 tgacgcgcac cctggtcgac tggatgggcc ggcaagccgc gcccccgcgc gtgctgctgt
     6961 caggctcggc cgtcggctgg tatggcgacc gcggcgagca atggctggcc gaagacagcg
     7021 cgccgggcaa cgccgacttc ggcagccgcc tgtgcgtggc ctgggagcaa gaggccgagc
     7081 gcgcgcgcca gtggggcgtg cgcgtggcgc tgctgcgcac cgcgcccgtg ctggccccgc
     7141 aggggggcat gctggcgcgg ctgctgccgc ctttcaggtt gggcctgggc gggcgcctgg
     7201 gcagcggcca gcagtggatg ccctggatcc atctggacga ccaggtggct ctgatggacc
     7261 acttgttgca caacgatgcc tgtagcggcc cctacaacgc ctgcgcaccc gaggcggtgc
     7321 gcaacaccga attcacccag accctggcgc gcacgctggg ccgcccggcc gtgctgcccg
     7381 caccggcctg ggtgctgcgc ctggcgctgg gcgaaatgtc ggtgctgctg ctcgggggcc
     7441 agcgcctggc gccgcgccgc acgcaggggg cagggtttgc ctggcgccat ccccagctgg
     7501 cagcggccct ggcgcagctg ctgcgaaaat catagtaaaa atcgtagcgt atggcgcgct
     7561 gatcctgggg tcgaaactcg tagccagtgc ctgtaacaat ggctcggcgc ctcatcaggg
     7621 tttggcgagc agtttctcca actgccccag aaacgccgtg ctttcgggct cgaccgtgct
     7681 cgggtagtgg gtgaccgaaa gccccgatcg gtcaatgagg tatttgttga agttccatcc
     7741 cggcactttg ccggtcatct gcgccagctg tttgtagagt gggttggcat ccgagccccg
     7801 caccgcagag cgcgcgaaca tcgggaagat cacgccatag gtgttgtagc aaaagtccgc
     7861 gatttccttg ttgtcgtttt tttcctggaa aaagtcgttc gagggaaagc ccagcacgac
     7921 caggccgcgg tctttgtact tctggaacag cgcctccagg ccctggtatt ggtacgtgaa
     7981 cccgcaatag ctggccgtat tgaccaccag gatcaccttg ccctggtact ggcacagcga
     8041 ttgcgggctg tcatcttgca atctgggaaa cctgtgcctg agcaccgccg ggcaaccaga
     8101 caaggcctgc tcgccggctg cggctgcggt cggtgccggc gggacactgg ggacgcccgc
     8161 gaaggccatc gggtgagaaa taacgagcaa aaacgccaac gcacgaccgg acatatacag
     8221 cacccgtggg agagacgatg gtttcattgt cgtgcagcgg ctctagtccc gtaataaccc
     8281 tcccgccaac cccggcgcgc cgcctgcccg cacgcgctgc ggccgtcacg gcgggtggcg
     8341 tgcgaacagc ttgccgcgcg cggtggcggt gatgacggat acagccgggt gtgtgatgcg
     8401 ccgctccac
//
LOCUS       DAOMTQ010000129         8374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1864_length_8374_cov_24.362904, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000129 DAOMTQ010000000
VERSION     DAOMTQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_8374_cov_24.362904"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..699)
                     /locus_tag="PK518_11825"
     CDS             complement(265..699)
                     /locus_tag="PK518_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRM49647.1"
                     /translation="MRALLDINVLIALLDAAHMHHARATQWLQQEIAHGWASCPLTQN
                     GCLRIMAQPAYPQALPLAAVASRLAQAAAHPAHAFLADDYSLLDAGQLHWQHLLGHRQ
                     ITDSYLLGLAVRHRCRFVSFDARLSLNVVPGARPEHLVTLQS"
     gene            complement(703..948)
                     /locus_tag="PK518_11830"
     CDS             complement(703..948)
                     /locus_tag="PK518_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49648.1"
                     /translation="MRTTVDLDYDTLIAAKEIARMENTSLGKVISRLVRQALTGNATA
                     QAASSPATSATGFVPFEARGVVVSNELIDRLRDNDGV"
     gene            complement(1044..2093)
                     /gene="mnmH"
                     /locus_tag="PK518_11835"
     CDS             complement(1044..2093)
                     /gene="mnmH"
                     /locus_tag="PK518_11835"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRM49649.1"
                     /translation="MSHHRPTRPADRHGFDAIIDARSPAEFAEDHIPGAINCPVLDDA
                     ERATVGTLYAQQGAFEARRVGGAMVAANLARHLRQQFADKPQGWRPLIYCWRGGMRSG
                     SMVQWLRLVGWDAQQLAGGYKAFRRHVIEQIAALVPQLDLRVLVGATGSAKTRVLQAL
                     ASQGAQVLDLEHCARHKGSLLGAMPGVEQPSQKNFETQIATALEGFDLSRPVYVEGES
                     ARIGRLSLPVPLVQRLRAGACLEIQATPEERLAYLLRDYAYLGDDPEALAQRLEALTE
                     LHGKEVVQRWQTWARARQLAPLFDELMRLHYDPHYGRSQARNFAQWQARRVVAATDLS
                     DAGIERLAQTVSASA"
     gene            complement(2142..2468)
                     /locus_tag="PK518_11840"
     CDS             complement(2142..2468)
                     /locus_tag="PK518_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HRM49650.1"
                     /translation="MQKPPRTWISNATPKQGELVRVRAQIEHTMESGLRTDAAGQIRP
                     RNIVTRFEARLGPTLLLAWEPGISVAPNPYIEFTFLARESGELHLLWKDDAGQTLSAQ
                     KTITLG"
     gene            complement(2490..2969)
                     /locus_tag="PK518_11845"
     CDS             complement(2490..2969)
                     /locus_tag="PK518_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HRM49651.1"
                     /translation="MRQPHPTRRSLVAAGAALALPGTVLAQAKAPLVGPLAPNPQAFK
                     QAMEQFIGKARPQADGLLLDVPVLADNPSGVPVKVKITLPLTEQDWCEEIIVLAELNP
                     SPLACRLQFTAAAGTAEASVRLRLAQSQTVHALARMKSGKVLATKQAVTVAASGCGM"
     gene            complement(2971..4308)
                     /locus_tag="PK518_11850"
     CDS             complement(2971..4308)
                     /locus_tag="PK518_11850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRM49652.1"
                     /translation="MKRRNFLISPPALALGAAAAPALALAAPAIISPQARILPRQGKG
                     PRIVICGGGWGGLTAARYLRELIPNADVVVLERNPSFWSGPMSNKWLVDIVGTDFVQH
                     DMLRPANRYGYQLLQTEVTGFERAQKLVRTTHGLVEYDYLILSGGIRDAWDAWFGDDQ
                     RAIEHTRRHYASAYIPNQQMFGLKQRVKDFKGGTLVMTLPPPPHRCPPSPYERACLIA
                     SHIKKNKIPGKIVILDPKPKIAPIGMGYKQAFEELYADVITHVPNARVQEVDPFNKKI
                     KTTAGELKFDEAILMPPHQAADMVWHAGLIGKDAATGKPTGWADMHPRLFHAHGDDSV
                     YFVGDLMGAISPQFGHYPKSGHVANYIGRIVAKYIAQRVAGQEVKPLLPDNLCYMMVN
                     TEPQEEISVKFEYEVDPSGKVLQTQIDMDVRSPDLVKEDFAWARGMFSDFLAI"
     gene            complement(4327..5373)
                     /locus_tag="PK518_11855"
     CDS             complement(4327..5373)
                     /locus_tag="PK518_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRM49653.1"
                     /translation="MTLLWAGLLLGCAFGIAARLGRFCLLRGLRQHGLAAARENGGAP
                     ALQAFALALAVALLASQALAWAGLADLAQAQVVRARFSVPGVLLGGLLFGCGMALARA
                     CGARALVLLAGGNLRALVTLLCLGLAAQATLTGVLAPLRQWLQGWGQITLAHATLAQQ
                     LQAGGLPPTATLALATGLPALALLAYALWQPALRQSAVQLWAAMAIGALVAAGWWITA
                     QVGVDPFEPTPLTSLSFIGPVAEGLLYLQLAVGRGASVGPAIVAGTLAGAFAAALLTR
                     SLRWEGFEQPARLAASALGGLLMGLGGVLAVGCSIGQGLSGLSTLAFASLPAAIGIAG
                     GASITLRLQAAHTR"
     gene            5713..6780
                     /gene="selD"
                     /locus_tag="PK518_11860"
     CDS             5713..6780
                     /gene="selD"
                     /locus_tag="PK518_11860"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRM49654.1"
                     /translation="MTAAPTASTLTPRLTSLSHGGGCGCKIAPGVLAQILQSSGAAGV
                     MPPELLVGIETADDAAVYQLNDSQALVATTDFFMPIVDDPYEFGRIAATNALSDVYAM
                     GGRPIMALALVAMPVNQLPVDVIAAIIRGGQDVCRAAGIPIAGGHTIDSVEPIYGLVA
                     MGLVHPDRVRRNAGARAGDLLVLAKPLGVGVYSAALKKEQLADAHYREMIASTTQLNT
                     PGPLLADLPGVHAITDVTGFGLAGHGLEMARGAGLSVRIDWARVPLLPGVVELAAQGF
                     VTGASGRNWAGYGADVALGAGLPATAQALASDPQTSGGLLVACAPDSVDEVLALFARE
                     GFARAAVIGGVEAGPARLELR"
     gene            6777..7847
                     /locus_tag="PK518_11865"
     CDS             6777..7847
                     /locus_tag="PK518_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HRM49655.1"
                     /translation="MSDLPAPAPQPGMTAGQGGLRELSPAYFGLVMATGIVSLVADML
                     GWPLLAHALLALNAVQYLVLWGLYLLRAWRYPRRFFGDMVEHARGAGYFTMVAATGIM
                     ASQCIVMLDNLGLGFALWALAALLWLLLTYTIFTAFTIRRDKPTLDKGISGAWLLAVV
                     STQALAVCSALLAARIGQPLRLELNLLALSMWLWGGMLYIWMMALIFYRYLFFPFSPG
                     DLSPPYWINMGAMAISTLAGSLLMLNAPQAPFLVSLLPFLKGFTVFYWAAGTWWIPML
                     LLLGIWRHGVRRFPLRYDPLYWGAVFPLGMYAACTWQMDKAMQFGFLNLLPRLFFAVA
                     LLAWLLAFVGMLRSVARQLRAP"
     gene            7930..>8374
                     /locus_tag="PK518_11870"
     CDS             7930..>8374
                     /locus_tag="PK518_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRM49656.1"
                     /translation="MALTEQELLAAIAGVTDPHTGKDFVSTRALRNVQISAGDVAFDV
                     ELGYPARSLIPALRSQFIAAAKAAPGVQNVSVNITSKVTAHAVQRGVQLLPGVKNIVA
                     VASGKGGVGKSTTAANLALALAAEGARVGVLDADIYGPSQPMMLGI"
BASE COUNT         1201 a         2757 c         3018 g         1398 t
ORIGIN      
        1 gtgtaattgg ggtcagattc gaattaaatt ttgactccag cgcttgctgg tattgcgtga
       61 gcagctcttt tttttgatgt cactggccgg gatgggtgcc cggcggcaca ctcacttcct
      121 tttgcttcga agcaaagaac agtgtctcgt ctgccggggc caaacccggc tagccacagc
      181 aaaaagagga gctgctcgcg cgccaccaac aagcgtaaag gcccaatttg cctatgcatg
      241 agtaaccgaa gccagacacc tatttcatga ttgcagagtc accagatgct ccggcctggc
      301 gcctggaacg acgttcaggc tcagccgggc gtcgaagctg acaaagcggc agcggtggcg
      361 cacggccagg cccagcaggt agctgtcggt gatttgccgg tgacccagca ggtgctgcca
      421 gtgcagctgg cccgcgtcca gcaggctgta gtcgtcggcc agaaaggcgt gcgcggggtg
      481 tgcggcggcc tgtgccaggc ggctggcgac ggcggccagc ggcagggcct gggggtaggc
      541 gggctgggcc atgatgcgca ggcaaccgtt ttgcgtgagc gggcaggagg cccagccatg
      601 ggcaatttcc tgttgcagcc attgcgtggc ccgggcgtgg tgcatgtgcg ccgcgtccag
      661 caaggcgatc aggacgttga tgtccagcag ggcgcgcatg gcctacaccc cgtcgttgtc
      721 gcgcaggcga tcgatcaact cgttgctgac cacgacgccg cgggcctcaa agggaacaaa
      781 gcccgtggcc gaggtggccg ggctgctggc ggcttgcgct gtcgcgttgc ccgtcagtgc
      841 ctggcgcacc aggcgtgaga tgaccttgcc caggctggtg ttttccatgc gggcaatttc
      901 cttggcggct atcaaggtgt cgtagtccag atcaaccgtg gtgcgcatgg cgtgcctcct
      961 tcttccacaa tcgcattgtc ttttgattct agtgcatcat gatgcgatcg catcaatgat
     1021 gattaattgg aatctgaccc caattaggcg gaggccgaga ccgtctgtgc caggcgctct
     1081 atcccggcgt ccgaaagatc agttgccgcc acgacgcggc gggcctgcca ctgcgcaaag
     1141 ttgcgcgcct gcgagcggcc gtagtgcggg tcatagtgca gccgcatcag ctcgtcgaac
     1201 agcggcgcca gttgtcgcgc gcgggcccag gtctgccagc gctgaaccac ctccttgccg
     1261 tgcagctcgg tcagcgcctc caggcgctgg gccagggcct cggggtcgtc gcccaggtag
     1321 gcgtagtcgc gcagcaggta ggccaggcgc tcctcgggcg tggcctggat ttccaggcag
     1381 gcgccggcgc gcaggcgttg caccaggggc acgggcaggg acaggcggcc gatgcgcgcg
     1441 ctctcgccct ccacatagac cgggcgcgac aggtcaaagc cttccagcgc cgtggcgatc
     1501 tgcgtttcga agtttttttg cgagggttgc tcaacgccgg gcattgcgcc cagcagcgag
     1561 cccttgtggc gcgcgcagtg ctccaggtcc agcacctgtg cgccctggct ggccagggcc
     1621 tgcagcacgc gtgtcttggc gctgccggtg gcgccgacca gcacgcgcag atcaagctgc
     1681 ggcaccaggg cggcgatctg ctcgatcaca tggcggcgga aggccttgta gccgccggcg
     1741 agttgctgcg cgtcccagcc caccaggcgc aaccattgca ccatggagcc gctcctcatg
     1801 ccgccgcgcc agcagtagat cagcgggcgc cagccctggg gcttgtcggc aaactgttgg
     1861 cgcaggtggc gcgccaggtt ggcggcaacc atggcgccgc ccacgcgccg ggcctcgaag
     1921 gcgccctgct gcgcgtagag ggtgccgacg gtggcgcgct cggcgtcgtc gagcaccggg
     1981 cagttgatgg cgccggggat atggtcttcg gcaaactcgg cgggcgagcg cgcgtcgatg
     2041 atggcgtcga agccgtggcg gtcggcgggg cgggtggggc ggtggtggga cacgggctgg
     2101 gctgcgatgg gcagggggcg cccactatag accagccagg atcagcccag cgtgatggtc
     2161 ttttgcgcgc tcagcgtctg gccggcatcg tccttccaga gcaggtgcag ctcgccgctt
     2221 tcgcgcgcca gaaaggtgaa ctcgatgtac gggttgggcg cgaccgagat gcccggctcc
     2281 caggccagca gcagggttgg ccccaggcgc gcctcgaagc gtgtgacgat gttgcgcggc
     2341 cggatctggc ctgcggcgtc ggtgcgcagg ccgctttcca tggtgtgttc gatctgcgca
     2401 cgcacgcgca caagctcgcc ttgcttgggc gtggcgttgc tgatccaggt gcggggtggt
     2461 ttctgcattg cgtcgtcctt ggtgcgtctt tacataccgc agccgctggc ggccacggtg
     2521 acggcctgtt tggtggccag caccttgccg cttttcatgc gcgccagagc gtggacggtt
     2581 tgcgactgcg ccaggcgcag ccgcaccgat gcctcggccg tgcctgccgc agccgtgaac
     2641 tgcaggcggc aggccagggg cgaggggttg agctcggcca gcacgatgat ctcttcgcac
     2701 cagtcctgct cggtgagggg cagggtgatc ttgaccttga ccggcacgcc gctggggttg
     2761 tcggccagta ccggcacgtc cagcagcagc ccatcggcct ggggtcgggc cttgccgatg
     2821 aactgctcca tggcctgctt gaatgcctgc gggttgggcg ccagcggccc gaccagcggc
     2881 gccttggcct gcgccagcac ggtgccgggc agggccaggg cggcgccggc ggcgaccagg
     2941 cttcttcggg tggggtgggg ctggcgcatg tcagatggcc aggaagtcgc tgaacatgcc
     3001 gcgcgcccag gcgaagtctt ccttcaccag gtcggggctg cgcacgtcca tgtcgatctg
     3061 ggtctgcagc accttgccgc tgggatcgac ctcgtactcg aacttgaccg agatttcttc
     3121 ctgcggctcg gtgttgacca tcatgtagca caggttgtcg ggcagcagcg gcttgacctc
     3181 ctggcctgcc acgcgctggg caatgtattt ggcgacgatg cggccgatgt agttggccac
     3241 atggccgctc ttggggtagt ggccgaactg tggcgagatg gcacccatga ggtcgccgac
     3301 aaagtagacg ctgtcgtcgc cgtgcgcgtg gaacaggcgc gggtgcatgt cggcccagcc
     3361 ggtgggtttg ccggtcgccg cgtccttgcc gatcaggccg gcgtgccaga ccatgtcggc
     3421 cgcctggtgc ggcggcatga ggatggcttc gtcgaacttc agctcgccgg ccgtggtctt
     3481 gatcttcttg ttgaacgggt ccacctcttg cacgcgggcg ttgggcacat gggtgatgac
     3541 gtcggcgtac agctcctcga aggcctgctt gtagcccatg ccgatggggg cgatcttggg
     3601 cttgggatcg aggatcacga tcttgccggg gatcttgttc ttcttgatgt gcgaggcgat
     3661 caggcaggcg cgctcgtagg gcgagggtgg gcagcggtgc ggcggtggcg gcagcgtcat
     3721 gaccagcgtg ccgcccttga agtccttcac gcgctgcttg aggccaaaca tctgctgatt
     3781 cgggatatag gcgctggcgt aatgccggcg cgtgtgctcg atggcgcgtt ggtcgtcgcc
     3841 aaaccaggcg tcccaggcgt cgcggatgcc gcccgagagg atcagatagt cgtattccac
     3901 caggccgtgc gtggtgcgca ccagcttctg ggcgcgctca aagccggtca cctcggtttg
     3961 cagcagctgg tagccgtagc ggttggccgg gcgcagcatg tcgtgctgca cgaagtcggt
     4021 gcccacgatg tccaccagcc acttgttgct catcgggccg gaccagaagc tggggttgcg
     4081 ctccagcacc accacgtcgg cattcggaat cagctcgcgc aggtagcgcg ccgccgtcag
     4141 gccaccccag ccaccgccgc agatgacgat gcgcgggccc ttgccctggc gcggcaggat
     4201 gcgcgcctgc gggctgatga tggccggcgc cgccagcgcc agcgcgggcg cggcggccgc
     4261 gcccagggcc agggcggggg ggctgatgag aaagttgcgg cgtttcatgg cggttctcct
     4321 tgggggtcaa cgggtatggg cggcttgcag ccgcagggtg atggatgcgc cgcccgcgat
     4381 gccgatggcg gcgggcaggc tggcaaaggc cagggtggac agcccggaca gcccctggcc
     4441 gatggagcag cccacggcca gcacgccgcc caggcccatc agcaggccgc ccagggccga
     4501 tgcggccagg cgcgcgggct gctcgaagcc ctcccagcgc aggctgcgcg tgagcagcgc
     4561 ggcggcaaag gcgcccgcca gcgtgccggc gacgatggcc gggcctacgc tggcgccccg
     4621 acccacggcc agttgcaggt acagcaggcc ttcggcgacg gggccgatga agctcagtga
     4681 tgtgagtggt gtcggctcga acggatccac gcccacctgg gccgtgatcc accatccggc
     4741 ggccaccagg gcgccaatgg ccatggcggc ccacagttgc accgcgctct ggcgcagcgc
     4801 cggttgccac agggcgtagg ccagcagcgc cagcgctggc aggccggtgg ccagggccag
     4861 cgtggccgtg ggcggcaggc cgccggcctg cagctgctgc gccagcgtgg catgcgccag
     4921 cgtgatctgg ccccagccct gcagccattg gcgcagcggc gccagcaccc cggtcagcgt
     4981 ggcctgcgcc gccaggccca gacacagcag cgtcaccagg gcgcgcaggt tgccgccggc
     5041 cagcagcacc agcgcacgcg cgccgcacgc gcgcgccaac gccatgccgc agccaaacag
     5101 cagcccgccc agcagcacgc cgggcacgga aaaacgggcg cgcaccacct gggcctgcgc
     5161 cagatcggcc aggcccgccc atgccagtgc ctgcgaggcc agcagcgcca cggccagcgc
     5221 cagcgcgaag gcctgcagcg ccggcgcgcc gccgttttcg cgggcggccg caaggccatg
     5281 ctggcgcagc ccgcgcagca ggcaaaaacg ccccaggcgc gcggcaatgc caaaggcgca
     5341 gcccagcagt aatccggccc acagcagcgt catgggcgcc cccggatgcc ggccagaccg
     5401 ggagccgcca gcagcgcggc cagccaggcg cgggggcgct ggcgctggtg gtgcatggcg
     5461 gacgagggcg gtggcatgcg ggttcctgtt ctggggcacg cgcacgcgcg ccatccggcg
     5521 ggatgaatat gggggagctg gctggggcgg tgatggcgcg ggtcattgcc tgggcagacc
     5581 cgcggggtgg cgcatgggac ggccgtcacg cggcacagcg ctgaagccgt cgatcatacc
     5641 gaacgccccc tcccgcggcc gcatgaatct gggatgatcc atgcccttgc ccactcccag
     5701 ctccgcatcg ccatgaccgc tgcccccact gcctccaccc tcacgccgcg cctgacctcg
     5761 ctatcgcatg gcggcggctg cggctgcaag atcgcgccgg gggtgctggc gcagatcctg
     5821 cagtccagcg gcgcggccgg cgtcatgccg ccggagctgc tggtgggcat agagacggcg
     5881 gacgatgcgg cggtctacca gctcaatgac agccaggcgc tggtggccac caccgacttt
     5941 ttcatgccca tcgtggacga cccgtatgag tttggccgca tcgccgccac caacgccctg
     6001 agcgatgtgt acgccatggg tggccggccc atcatggcgc tggcgctggt ggccatgccg
     6061 gtgaaccagc tgcccgtcga tgtcatcgcc gccatcattc gcggcgggca ggatgtatgc
     6121 cgcgccgccg gcattcccat cgcgggcggg cacaccatcg actcggtcga gcccatctac
     6181 ggcctggtcg ccatgggcct ggtgcacccg gatcgcgtgc gccgcaacgc cggcgcgcgg
     6241 gcgggcgacc tgctggtttt ggcgaagccg ttgggcgtgg gggtgtattc ggcggccttg
     6301 aagaaggagc agctggccga cgcgcattac cgcgagatga tcgccagcac cacgcagctc
     6361 aacacccccg gcccgctgct ggcggatctg cccggcgtgc atgccatcac cgacgtgacc
     6421 ggcttcggcc tggccggcca tggcctggag atggcgcgcg gcgccggcct ctcggtgcgc
     6481 attgactggg cgcgcgtgcc gctgctgccc ggcgtggtgg agctggcggc gcagggcttt
     6541 gtgaccggcg ccagcggccg caactgggcc ggctatggcg ccgatgtggc gctgggcgcg
     6601 ggcctgccgg ccacggccca ggccctggcg agcgacccac agacatcggg cggcctgctg
     6661 gtggcctgcg cgccggacag cgtggacgag gtgctggcgc tgtttgcgcg cgagggcttt
     6721 gcccgcgccg cggtgatagg cggcgtagag gccggaccgg cgcggctgga gctgcgttga
     6781 gcgacctgcc cgcgcccgcg ccgcagcccg gcatgacggc cgggcagggc gggctgcggg
     6841 agctgtcgcc ggcctatttc ggcctggtca tggccacggg catcgtgtcg ctggtggccg
     6901 acatgctggg ttggccgctg ctggcgcatg cgctgctggc gttgaacgcc gtgcagtatc
     6961 tggtgctgtg ggggctgtac ctgctgcgcg cctggcgcta cccgcggcgc ttctttggcg
     7021 acatggtgga gcatgcgcgc ggcgccggct acttcaccat ggtggcggcc acgggcatca
     7081 tggccagcca gtgcatcgtg atgctggaca acctgggcct gggctttgcg ctgtgggcat
     7141 tggcggcgct gttgtggctg ctgctgacct acaccatctt caccgccttc accatccgcc
     7201 gcgacaagcc cacgctggac aagggcatca gcggcgcctg gctgctggcc gtggtgtcca
     7261 cgcaggcgct ggccgtgtgc agcgcgctgc tggccgcgcg cataggccag ccgctgcggc
     7321 tggagctgaa cctgctggcg ctgtcgatgt ggctgtgggg cggcatgctc tacatctgga
     7381 tgatggcgct gatcttctac cgctacctgt tctttccgtt ctcgcccggc gacctgtcgc
     7441 cgccgtactg gatcaacatg ggggccatgg ccatttccac gctggccggc tcgctgttga
     7501 tgctgaacgc gccgcaggcg ccgttcctgg tgtcgctgct gcccttcctc aagggcttca
     7561 cggtgttcta ctgggccgcc ggcacctggt ggattcccat gctgctgttg ctgggcatct
     7621 ggcgccatgg cgtgcgccgc tttccgctgc gctacgaccc tctgtactgg ggcgcggtgt
     7681 ttccgctggg catgtatgcg gcctgcacct ggcagatgga caaggccatg cagttcggct
     7741 ttctgaacct gctgccccgg ctgttctttg ccgtggcgct gctggcctgg ctgctggcct
     7801 tcgtcggcat gctgcgcagc gtggcgcgcc agctgcgcgc cccatagctg ctggcgatag
     7861 gcacaggcgg ggaggtgcca aggccgcttc gctagactcg gccgtcttcc ctttgcaaac
     7921 cacacagcca tggcattgac agaacaagag ctgctggcgg caatcgccgg cgtcacagac
     7981 ccccacacgg gcaaggactt cgtcagcacg cgcgcgctgc gcaatgtgca gatcagcgct
     8041 ggcgatgtcg cctttgacgt ggagctgggc taccccgcca ggagcctgat cccggcgctg
     8101 cgcagccagt tcatcgccgc ggccaaggca gcgcccggcg tgcagaacgt ctcggtcaac
     8161 atcacctcca aggtcacggc gcacgccgtg cagcgcggcg tgcagctgct gccgggcgtg
     8221 aagaacatcg tcgccgtggc ctcgggcaag ggcggcgtgg gcaagagcac caccgccgcc
     8281 aacctggcgc tggcgctggc ggccgaggga gccagggtgg gcgtgctgga cgccgacatc
     8341 tacggcccca gccagcccat gatgctgggc atca
//
LOCUS       DAOMTQ010000130         8374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1865_length_8374_cov_23.528068, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000130 DAOMTQ010000000
VERSION     DAOMTQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1865_length_8374_cov_23.528068"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PK518_11875"
     CDS             <1..576
                     /locus_tag="PK518_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HRM49657.1"
                     /translation="PRYTPTTCFETFPFPAGLTPADTAHQRTEALEGGALIPSGLAED
                     FKQKMPLAPTQQAQAAIKNIEARAAASAIAQAAQRLDQLRRNWLNPPEWTRAVPEAVP
                     LGMDASPYPDRIEPRPNLSEADAKALQKRTLTNLYNQRPAWLAQAHAQLDAAVAAAYG
                     WQDWRADMPDDEILRRLLALNHERSRTADAP"
     gene            573..839
                     /locus_tag="PK518_11880"
     CDS             573..839
                     /locus_tag="PK518_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49658.1"
                     /translation="MSRPRPATLAEIGDGQDRLVDVHGPEVHPDLRRAVLARMQPGDQ
                     LWRCPRRGAPRGLLGLLGMGQRDVVIEWWLLDAEGDLIEAFWEE"
     gene            complement(1077..1418)
                     /locus_tag="PK518_11885"
     CDS             complement(1077..1418)
                     /locus_tag="PK518_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine tRNA ribosyltransferase"
                     /protein_id="HRM49659.1"
                     /translation="MQHLEGQVIWPADTASDGAGLTLTLTVQLRDVALQDIAAPLVAE
                     HVAPVAAPAAGQASAFRLAWRTSAAAPGQGAPQLALQARVVDASGRLRYISTEHVAAA
                     PGARIRLQRVG"
     gene            complement(1405..2403)
                     /locus_tag="PK518_11890"
     CDS             complement(1405..2403)
                     /locus_tag="PK518_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HRM49660.1"
                     /translation="MILVVSYPGEEHTDAVCARLAAAGEPVHRLNLADFPARLPLACA
                     WTSQAAPALRLLGAEPIDLAAVDAVWWRRVQPFEIDPGVGPADRRQFVHSETEQAVLG
                     MLDSIDAPWINPRLADDAAHRKPLQWTQAAAVGLTLPPTCVTTAPDEARAFCAAHHSA
                     GVVFKPFLASIADWRETRLVEAEDLARLELVRLAPVIFQRRIQGVDLRVTVIGDALFA
                     AEIDARDSSYPVDMRMVIGEAKIRAVDLPPALSQRLLALMRRLGLVYGAIDLKQDEHG
                     DYYFLEVNPAGQWQFVESRAGLPITQAHADALRRMASAQRRRRGKTARSTQDHATP"
     gene            complement(2400..3722)
                     /locus_tag="PK518_11895"
     CDS             complement(2400..3722)
                     /locus_tag="PK518_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HRM49661.1"
                     /translation="MATRRKTEGGSTGAGGADPCECRAGPLAGTAFVGFRGLHGATGK
                     WLHYADVDGQAIFEGDIVLGSVADMQADESGNPVLFSVGITSPDGVQFRWAGGRVPYE
                     VDPALPNQARITDAIAHWESNTVIRFVLRTAANAAQHPDYVRFVPGGGCSSHVGRRGG
                     MQAITLGAGCTTGNTIHEIGHAVGLWHEQSREDRDQFVQVNWANIDPAMQHNFNQHIQ
                     DGDDLGAYDFGSIMHYPGTAFSINGQPTLVPRVALPPGVVMGQRTGLSAGDIAGVLAM
                     YPNVRPTIKEVGKDPIRDTIKEVRKDVIRDTIKEVRKDPIQDTVKEVRKDPIRDTAKE
                     LRKDPITDPITVKEVGRDPIGPIGPGPVINPAIRDYSAPFVLAGASRQPLGNDLGGGL
                     GDALQQAAAQVDQLAAAHEEAQAMADALAQELEQAVAQWQALGGEGGA"
     gene            complement(3936..4478)
                     /locus_tag="PK518_11900"
     CDS             complement(3936..4478)
                     /locus_tag="PK518_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49662.1"
                     /translation="MLDAAPWPDAQPAPAAPWSRTEDALPPEIARIAEGAVTLFKGGL
                     MNAGRQTWYALDLQRRCFLAVLRQSGAGLAPRPGARPVATCPAATESLHQASASAAQC
                     QAFAALAQRLLACADAQDGQPEPLPQGPLRRSLLLLHQGQALALPGGAAAQALRAEII
                     WLIQQPLQQELLGPDPHAGA"
     gene            complement(4504..5919)
                     /locus_tag="PK518_11905"
     CDS             complement(4504..5919)
                     /locus_tag="PK518_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HRM49663.1"
                     /translation="MTAHSHSHAQAHSCTIKTVAVAELAAAADRAVQINPLNAPSIQM
                     VRALGMPAEMPVQARLSVLTKKYWGSKGVKLTVGFMDSPPADLRARILSHMNAWGAWS
                     NVRFVETKTNPQVRIARTAGDGYWSYLGTDVLLIPANAPTMNLDGFTMNTPDSEFYRV
                     VRHETGHTLGFPHEHMRKEIIDRIDKQKAIAHFMQTQGWSEQEVIQQVLTPLAQSALI
                     ATAQADPTSIMCYWLPASIMKDGQAVSGGNDINALDAQFSGLIYPGPVAPSCIWPNGK
                     AYFFKGKQYLRYDPKADKTDAGYPKPMAGNWPGFPTTFSNKVDAFVLWGGGKAYFFKG
                     KQYLRYDVVADHVDPGYPRPIAGNWPGMWPEKIDAAICWPGNGKAYFFKGDQYLRYDI
                     AGDKTDAGYPRPIAGNWPGFPADFAAGVQAAAVWNNGKAYFFKGSQYLRYDIAADKVD
                     AGYPKPIAGNWPGFWPGGLDA"
     gene            complement(6085..>7077)
                     /gene="holA"
                     /locus_tag="PK518_11910"
     CDS             complement(6085..>7077)
                     /gene="holA"
                     /locus_tag="PK518_11910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRM49664.1"
                     /translation="LYTLHGDEALLQQEALDAIRAVARAQGYTERSSFTVAGAHFDWS
                     AVLAAGGSLSLFADRQIVEIRIPSGKPGKDGGAALQQIAQAAQGNDSVLTLVVLPRLD
                     KATRTGAWFSALEAHGATVQIDPVERAQLPAWIAQRLAAQGQRVAAGEEGQRTLQFFA
                     DRVEGNLLAAHQEIQKLALLHPQGELAREQVEAAVLNVARYDVFKLSEAVLSGQVART
                     QRMLDGLQAEGEAEVLVHWALAEDIRALKRVKDAMNAGKPLPMALRENRIWGAKERLY
                     QRLLPQASDAQLARLLQSAHVVDGIVKGLKQADWPQDGWQALSRLALQLCRLGR"
     assembly_gap    7078..7177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7178..7670)
                     /gene="lptE"
                     /locus_tag="PK518_11915"
     CDS             complement(<7178..7670)
                     /gene="lptE"
                     /locus_tag="PK518_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRM49665.1"
                     /translation="MRRRSLLACLAVAPLTACGFRLRGVPQFAFRTLYVQAPEGSAMA
                     RELVRTLQGAGGNLTVLTDPAQLPQAEAVLELPGEQQERIAVGMSAAGQIRELQLRLR
                     LRFRLRGQQGQEWIPETELRQQRDISYNETLALSKEGEEAMLFRNMRTDMVQQLMRRL
                     AAVK"
     gene            complement(7670..>8374)
                     /locus_tag="PK518_11920"
     CDS             complement(7670..>8374)
                     /locus_tag="PK518_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM49666.1"
                     /translation="GADTARLYTMFTAPPELTLEWNDAAVEGSYRFLRRVWNFGVKLS
                     AIDREAASASTAGVTSLNDVEFGKEAKALRLEIHTVLKQVEYDYQRMQYNTVVSGAMK
                     MINALEDFKAQDSAGAAVALIEGFGILLRVLYPATPHITHALWTELGYAGSLGDLLDA
                     AWPQVDAGALVQDELELMLQVGGKLRGAIRVPASADKAAIEAAALASPDFQKFAEGKA
                     PKKVIVVPGRLVNVVL"
BASE COUNT         1187 a         2845 c         2923 g         1319 t
ORIGIN      
        1 ccgcgctaca cccccaccac ctgcttcgaa accttcccct tccccgccgg cctgaccccc
       61 gcggacacgg cccaccagcg caccgaggcc ctggagggcg gcgcgctgat cccctccggc
      121 ctggcggaag atttcaagca aaaaatgccg ctagcgccca cccagcaagc gcaagcagct
      181 atcaaaaaca tagaagcacg cgccgccgcc agcgccatcg cccaggccgc ccagcgcctg
      241 gaccagctgc gccgcaactg gctcaacccg cccgagtgga cacgggccgt gcctgaggcc
      301 gtgccgctgg gcatggacgc ctcgccctac cccgaccgca tcgagccgcg ccccaacctg
      361 agcgaagccg acgccaaggc cctgcaaaaa cgcaccctga ccaacctcta caaccagcgc
      421 cccgcctggc tggcccaggc ccacgcgcaa ctggacgctg ccgtggccgc ggcctacggc
      481 tggcaggact ggcgcgccga catgcccgac gacgaaatcc tgcgccgcct gctggcgctg
      541 aaccacgagc gcagccgcac cgcagacgcc ccatgagccg cccccgcccc gcaacgctcg
      601 ccgagatcgg cgacggccag gaccgcctgg tcgatgtcca cggccccgag gtgcatcccg
      661 acctgcgccg cgccgtgctg gcgcgcatgc agcccggcga ccagctgtgg cgctgcccgc
      721 gccgcggcgc cccgcgcggc ctcctcgggc tgctgggcat gggccagcgc gacgtggtga
      781 tcgaatggtg gctgctggac gccgagggcg atttgatcga ggcgttctgg gaggagtagg
      841 ccgccagaaa aatcgatctg gcgcccgagg aacaagcctt ttgatccggc aagtagcggt
      901 cgttggctgt cgtcatcccg gcgcaggccg ggatccagca tccggcatcc aggctggcca
      961 agggcatccc tggatcccgg cctgcgccgg gatgacggtc aacgcttggt tgcggagtcg
     1021 cctggatgac ggtcagcgct tggttgccgg atccattgtt acggggccaa gtcgcttcac
     1081 cccacccgct gcagcctgat cctggcgccc ggagcggcgg cgacatgctc ggtgctgatg
     1141 tagcgcaggc ggccgctggc gtccaccacg cgtgcctgca gggccagctg gggcgcgccc
     1201 tggccgggtg cggcggcgct ggtgcgccag gcgagccgga aggcgctggc ctggccggcg
     1261 gcgggggccg caaccggggc gacatgttcg gccaccaggg gcgcggcgat gtcctgcagc
     1321 gccacgtcgc gcaactgcac ggtgagggtg agggtgagcc cggcgccgtc gctggcggtg
     1381 tcggcgggcc agatgacctg gccttcaagg tgttgcatgg tcttgggtgc ttctggctgt
     1441 tttgccgcgg cgccggcgct gcgcgctggc catgcggcgc agggcgtcgg catgggcctg
     1501 ggtgatgggc aggccggcgc gcgattctac gaactgccat tggccggcgg ggttgacctc
     1561 gaggaagtag tagtcgccgt gctcgtcctg cttcaggtcg atggcgccgt agaccagccc
     1621 cagccggcgc atcagcgcca gcagccgctg gctcagtgcc ggcggtaggt ccacggcgcg
     1681 gatttttgcc tcgcctatga ccatgcgcat gtccacgggg tagctgctgt cgcgcgcgtc
     1741 gatctcggcg gcaaagaggg catcgcctat caccgtgacg cgcagatcca caccctgtat
     1801 gcggcgctgg aagatcaccg gcgccagccg caccagctcc agccgcgcca ggtcttcggc
     1861 ctccaccagg cgtgtctcgc gccagtcggc aatgctggcc aggaagggct tgaacaccac
     1921 gccggcgctg tggtgcgcgg cgcagaaggc gcgcgcctcg tcgggcgcgg tggtcacgca
     1981 ggtgggcggc agcgtcaggc cgacggcggc ggcctgcgtc cactgcagcg gcttgcggtg
     2041 cgcggcgtcg tcggccaggc gcgggttgat ccagggcgcg tcgatggagt cgagcatgcc
     2101 cagcacggcc tgctcggtct cgctgtgcac gaactgccgg cggtctgccg ggccgacgcc
     2161 ggggtcgatc tcgaacggct gcacgcgccg ccaccagacg gcgtccaccg ccgccaggtc
     2221 gatgggctct gcgcccagca gccgcagcgc cggcgccgcc tggctagtcc aggcgcaggc
     2281 cagcggcagc cgggcgggaa agtcggccag attcagccgg tgcaccggct cgcccgccgc
     2341 ggccaggcgc gcgcagacgg cgtcggtgtg ctcctcgccc gggtaggaga cgaccaggat
     2401 catgcgccgc cctcaccccc cagcgcctgc cattgcgcca cggcctgctc cagctcttgc
     2461 gccagggcgt cggccatggc ctgggcctct tcgtgggccg cggccagctg atcgacctgc
     2521 gcggcggcct gctgcagcgc atcgcccagc ccgccgccaa ggtcgttgcc cagcggctgg
     2581 cggctggcgc cggccagcac gaagggggcg gagtaatcgc gaatggccgg attgatcacc
     2641 gggcccgggc ctatggggcc tatggggtcg cggccgacct ccttgaccgt gatcgggtcg
     2701 gtgatggggt ccttgcgcag ctccttggcc gtgtcgcgga tgggatcctt gcgcacctcc
     2761 ttgacggtgt cctggatggg gtccttgcgc acctccttga tggtgtcgcg gatgacgtcc
     2821 ttgcgcacct ccttgatggt gtcgcggatg gggtccttgc ccacctcctt gatcgtcggc
     2881 cgcacattgg ggtacatggc cagcacgccg gcaatgtcac cggccgacag gccggtgcgc
     2941 tgccccatca ccacgcccgg cggcagggcc acgcgcggca ccagggtggg ctggccgttg
     3001 atggaaaagg ccgtgcccgg gtaatgcatg atggagccga agtcgtaggc gcccaggtcg
     3061 tcgccgtcct gtatgtgctg gttgaagtta tgctgcatcg ccgggtcgat gttggcccag
     3121 ttgacctgca cgaactggtc gcggtcctcg cggctttgct catgccacag gcccacggcg
     3181 tggccgatct cgtggatggt gttgccggtg gtgcagcccg cgcccagcgt gatggcctgc
     3241 atgccgccgc gccgccccac atgcgaggag cagccgccgc cgggcacgaa gcgcacatag
     3301 tccgggtgct gcgcggcgtt ggccgccgtg cgcagcacga agcggatcac ggtgttggac
     3361 tcccaatgcg caatggcatc ggtgatgcgc gcctggttgg gcagcgccgg gtccacctca
     3421 tagggcacgc ggccgccggc ccagcggaac tgcacgccgt ccgggctggt gatgcccacc
     3481 gagaacagca ccgggttgcc ggattcgtcg gcctgcatgt cggccacgct gccaagcacg
     3541 atgtcgccct cgaagatggc ctggccatcg acatcggcat agtgcagcca cttgcccgtg
     3601 gccccatgca gccccctgaa gcccacgaag gccgtgcccg ccagcgggcc ggcgcggcat
     3661 tcacaggggt cggcgccgcc agcacccgtc gatccgcctt cggttttgcg tctggttgcc
     3721 atgatgattt acctcgctga agtgggcgcc aatcggctac gaggggggac tgcctgtagt
     3781 gaccagcgcg atgaatgcct gcttcatgct ccttcaaggt cagatcccga aaccggctgc
     3841 gaatccatgg ccagcaaggg caggatgcac atggttctga tcgttgcacg acagtaagtc
     3901 gtgactaacg agtgtcctac cgggcttggc gcgcgtcaag cgccggcatg tggatcgggc
     3961 cccagcagct cctgctgcag cggctgctgg atcagccaga tgatctcggc gcgcagcgct
     4021 tgcgcggcgg ccccgcccgg cagcgccagc gcctgcccct ggtgcagcag cagcaggctg
     4081 cggcgcagcg ggccctgggg caggggctcg ggctggccgt cctgtgcgtc ggcacaggcc
     4141 agcaggcgct gcgccagcgc agcaaaggcc tggcactgcg cggcgctggc gctcgcctgg
     4201 tgcagtgatt cggtggccgc cgggcatgtg gccaccggcc tggcgccggg ccgcggggcc
     4261 aggccggcac cgctttggcg cagcacggcc agaaagcagc ggcgctgcag atcgagtgcg
     4321 taccaggtct gccggccggc gttcatcagc ccgcccttga acagggtgac cgcgccctcg
     4381 gcaatgcgcg cgatctcggg cggcagggcg tcctcggtgc gcgaccaggg cgcggcgggc
     4441 gccggctgtg cgtcgggcca tggcgccgcg tcaagcacgg gcccgaaggg ccccggtggc
     4501 ccatcaggca tccagccccc cgggccagaa acccggccag ttgccggcaa tcggcttggg
     4561 gtagccggca tcgaccttgt cggcggcgat gtcgtagcgc aggtactggc tgcccttgaa
     4621 gaaataggcc ttgccgttgt tccacacggc ggcggcctgc acgccggcgg caaagtcggc
     4681 cgggaagccc ggccagttgc cggcaatcgg cctggggtag ccggcatcgg tcttgtcgcc
     4741 ggcgatgtcg tagcgcaggt actggtcgcc cttgaagaaa taggccttgc cgttgccggg
     4801 ccagcagatg gcggcgtcta tcttctcggg ccacatgccg ggccagttgc cggcgatggg
     4861 gcgcgggtag ccgggatcga catggtcggc caccacgtcg tagcgcaggt attgcttgcc
     4921 cttgaagaag taggccttgc cgccgcccca gagcacgaag gcatcgacct tgttgctgaa
     4981 ggtggtggga aagccgggcc agttaccggc catgggcttg ggatagccgg cgtcggtctt
     5041 gtcggccttg gggtcgtagc gcaggtattg cttgcccttg aagaaatagg ccttgccgtt
     5101 gggccagatg cacgatggcg ccaccgggcc ggggtaaatg aggccggaga actgggcatc
     5161 aagcgcgttg atgtcgttgc cgccgctgac ggcctggccg tccttcatga tgctggccgg
     5221 cagccagtag cacatgatgg aggtcgggtc ggcctgggcg gtggcgatca gggccgattg
     5281 cgccagcggc gtcagcacct gctggatcac ttcctgctcg ctccagccct gggtttgcat
     5341 gaaatgggca atggcctttt gcttgtcgat gcggtcgatg atttccttgc gcatatgctc
     5401 atgcggaaag cccagcgtgt ggccggtctc gtggcgcacc acgcggtaga actccgagtc
     5461 cggggtgttc atggtgaagc cgtccaggtt catcgtcggg gcgttggccg gtatcaacag
     5521 cacgtcggtg cccaggtagg accagtagcc gtcgcccgcg gtgcgggcaa tgcgcacctg
     5581 ggggttggtc ttggtctcga caaagcgcac gttgctccac gcgccccagg cgttcatgtg
     5641 gctcaggatg cgcgcgcgca ggtcggccgg cgggctgtcc atgaagccga ccgtgagctt
     5701 gacgcccttg gagccccagt attttttcgt cagcacgctc aggcgcgcct ggaccggcat
     5761 ctcggcgggc atgccgagcg cgcgcaccat ctggatcgat ggggcattca gcgggttgat
     5821 ctgcaccgcc ctgtccgctg ccgccgccag ttcggcaacg gctaccgtct tgatggtgca
     5881 tgagtgggcc tgggcgtggg aatgggaatg cgctgtcata caacctcctg ccgcaaaagc
     5941 gtgagtcgaa cgattaggcg cgcggtgtcg gccgccggca tgcctgcgcg ccagtgtcag
     6001 catttactcc catgccatgg cgctgtcaac ggtggctgac ggcggtgctc aggcaaggct
     6061 ccggcaaggc gccgggcagc ggcttcagcg ccccaggcgg cacagctgca gcgccaggcg
     6121 cgacagcgcc tgccagccgt cctgtggcca gtcggcttgc ttcaagccct tgacgatgcc
     6181 gtccaccaca tgcgccgatt gcagcaggcg cgccagctgc gcgtcgctgg cctggggcag
     6241 caggcgttgg tagagccgct ccttggcgcc ccagatgcgg ttctcgcgca gcgccatggg
     6301 caggggcttg ccggcgttca tggcgtcctt cacgcgcttc agggcgcgta tgtcctcggc
     6361 cagtgcccag tgcaccagca cctcggcctc gccctcggcc tgcaggccgt caagcatgcg
     6421 ctgggtgcgc gccacctggc cggagagcac ggcctcggag agcttgaaca cgtcgtagcg
     6481 cgcgacgttg agcacggcgg cctcgacctg ttcgcgcgcc agctcgccct gggggtgcag
     6541 cagggccagc ttctggatct cctggtgcgc ggccagcagg ttgccctcaa cgcggtcggc
     6601 aaagaattgc agcgtgcgct ggccctcctc gccggcggcc acgcgctggc cctgggcggc
     6661 caggcgctgc gctatccagg cgggcagctg ggcgcgctcc acggggtcga tctgcaccgt
     6721 ggcgccatgt gcttcgagcg ccgagaacca ggcgccggtc ctggtggcct tgtccaggcg
     6781 cggcagcacg accagagtga gcacgctgtc gttgccctgc gccgcctggg caatctgctg
     6841 cagggcggcg ccgccgtcct tgccgggctt gcccgagggg atgcggatct cgacgatctg
     6901 ccggtcggca aacagggaga gcgacccgcc agcggccagc accgcactcc agtcgaaatg
     6961 cgcgccggcc acggtgaagc tgctgcgctc ggtgtagccc tgggcgcgcg ccacggcgcg
     7021 gatggcgtcc agcgcctcct gctgcaacag ggcctcgtcg ccgtgcaggg tgtagagnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct tcaccgcggc caggcggcgc
     7201 atcagttgct gcaccatgtc ggtgcgcata ttgcgaaaca gcatggcctc ctcgccctcc
     7261 ttggacaggg ccagggtttc gttgtagctg atgtcgcgct gctggcgcag ctcggtctcg
     7321 ggtatccatt cctggccctg ctggccacgc agccgaaagc gcaggcgcag gcgcagctgc
     7381 agctcgcgga tctggccggc ggcgctcatg cccacggcaa tgcgctcctg ctgctcgccg
     7441 ggcagctcca gcacggcctc ggcctggggc agctgggcgg ggtcggtcag caccgtgagg
     7501 ttgccgcccg caccctgcag cgtgcgcacc agctcgcgcg ccatggccga gccttcgggc
     7561 gcctgcacgt acagcgtgcg aaaggcgaac tgcggcacgc cgcgcaggcg aaagccgcag
     7621 gcggtgagcg gcgccacggc caggcaggca agcaggctgc gtctgcgcat cacagcacca
     7681 cgttgaccag gcggccgggc acgacgatga ccttcttggg cgccttgcct tcggcaaatt
     7741 tctggaagtc ggggctggcc agcgccgcgg cctcgatggc ggccttgtcg gcgctggccg
     7801 gcacgcggat cgcgccgcgc agcttgccgc ccacctgcag catcagctcc agctcgtcct
     7861 gcaccagggc gccagcgtcc acctggggcc aggcggcgtc cagcagatcg cccagggagc
     7921 cggcgtagcc cagctcggtc cacagcgcgt gcgtgatgtg cggcgtggcc gggtagagca
     7981 cgcgcagcag gatgccaaag ccctcgatca gcgccacggc ggcgcctgcg ctgtcctggg
     8041 ccttgaagtc ctccagcgcg ttgatcatct tcatggcgcc ggagaccacg gtgttgtact
     8101 gcatgcgctg gtagtcgtac tcgacctgct tgagcacggt gtggatctcc aggcgcaggg
     8161 ccttggcctc cttgccaaac tcaacgtcat tcaggcttgt aacgcccgct gtacttgcgc
     8221 tggcagcttc tctatcgata gcagacagct tcacgccgaa gttccacacc cggcgcagga
     8281 agcggtagct gccctccacc gccgcgtcgt tccactccag cgtcagctcg ggcggcgcgg
     8341 tgaacatggt gtacaggcgc gcggtgtcgg cgcc
//
LOCUS       DAOMTQ010000131         8149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1951_length_8149_cov_24.836422, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000131 DAOMTQ010000000
VERSION     DAOMTQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1951_length_8149_cov_24.836422"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PK518_11925"
     CDS             <1..1131
                     /locus_tag="PK518_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoproteinase ArgC"
                     /protein_id="HRM49667.1"
                     /translation="VARTAALLPWRPTARGTQVAALRFESPGARGLRLGVLVQALPPG
                     AVLRFYAAAAEDAVQLSAEQLQAVAERNASAGADATLARTYWSPDFGGAQTTLEVEIP
                     EGAAPAAVRLAVPRLSHFTSTAAEAEAAFTTKAAVAGCLVDVSCHPDYLEQSRSVARM
                     TYVAEDGGAFYCTGTLLNDMAASGTPYFLSAQHCIPSQVVASTLVTDWLYRSASCNSA
                     AENPASRRLTGGARLLYASAGTDTAFMRLNDPLPAGVVYAGSYYGGVPIGTALASLHH
                     AEGGLQSISLGSVLRYSNCGDESCTATSGETGNYLALTWQQGSTEGGSSGSGVFVTIG
                     ARRYLAGQLHGGSASCARPDGTDYYGRFDLSYRAALKNWLNP"
     gene            complement(1138..3984)
                     /locus_tag="PK518_11930"
     CDS             complement(1138..3984)
                     /locus_tag="PK518_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRM49668.1"
                     /translation="MTPPPQPAAARHAVGIDLGTSHTVVAHVPLDGGVADIALLPLAQ
                     RISAAEVQEQPLLPSTRYQAAPGELGDAWRQPWPAQDATEQTPAVIGRWARDLGAAVP
                     GRLVASAKSWLSHAGVDRTAPILPWGAGQDVAKVSPLQASSSYLAHVRAAWDATHPDA
                     PLAEQLVVLTVPASFDEGARALTLQAAQLAGLPRVQLLEEPKAAFHDWLVLQGSQLAE
                     QLQDSRLVLVLDVGGGTTDLTLIRVEAAPGGGLPLLTRTAVGEHLMLGGDNMDLALAH
                     GLEPQFAGEGQRLPAARFAQLVQRCRLAKEQLLAANAPESVAVTLLGGGSQLLAQTRS
                     ATLTRDAVRQAVVEGFLPPAQITDQPARRQGALRTLSLPYPADAAISRHLALFLAQHA
                     AGELPDTLLLNGGVFHAHALVQRLTEQLGEWRGAPLRVLHNPHPDWAVARGAAAHGLA
                     EYESKRPPSLTGQAQAASKTIVPRIGGGAARSYWLVLPGQAGAAPQGICLLPRGTEEG
                     TRMVLAGRRFALRLGQPVRFALVARSAGQAQAGQIAALDGEGWVELPPLATVLPAPEG
                     RDKAQVQVELQACISEVGVLELRCIDTQDAGQSWLLPFAVRGAGPHPLPEGEGAKPPA
                     DRSPSPLWGEAGVRGALDAAIAQIDRIFGNQTQDVSPKEVRQLRATLEKTLGPRESWP
                     LPLLRTLFDALLARAKRRRRSAEHERVWLNLAGWCLRPGLGAELDGWRLQQLWPLYAQ
                     GLGHSQEGANWTEWWVLWRRVAAGLDEAQQMQVLEDVAGRMQQTVQRGAKSRWGSYDD
                     MLRLFAAMEAVPWQYRQEMGRWMLERLKRPDEPPHTWWAIGRLAARAPLAANAHLVMP
                     PEAAQEFLQATLAQDWRKNEPAMFAAVQIARMTGERLLDLDEAQRARVLDKLRASGAP
                     ERWLAQVAQVQEMNAEDRRLSLGDSLPPGLVLVG"
     gene            complement(4001..4885)
                     /locus_tag="PK518_11935"
     CDS             complement(4001..4885)
                     /locus_tag="PK518_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM49669.1"
                     /translation="MHSFFKHWLAAGAIAALALPTWAADATLAKIQSSGQLTIGYRLD
                     AAPLSYAGEGGKPVGYAVELCQQLAARIQSQLKLPALQLHYVPVTIAERFDRIADGKI
                     QLECADSTNTKARRERVAFGLTYYYAGARMLVRANDTRQQLAQMGQARVAVINGSTGQ
                     QVSERQPGLTLVTVASTEEGAKAVAQGRADAFVSDDISLIDQARLLKGTVKVVGARLS
                     VEPLAPLVSKYAPDFQNLVNQAMKDLYRDGTARQIYKKWFEQPLPGRGYSLDLPPDML
                     LNDTFRRPDGFVTDWTVL"
     gene            complement(5001..6884)
                     /locus_tag="PK518_11940"
     CDS             complement(5001..6884)
                     /locus_tag="PK518_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRM49670.1"
                     /translation="MTIETIADSALPTGATARFSIGIDLGTTHCALSYVDMQASDGEQ
                     VVQQVLDVPQLTAPASVEGRPLLPSFLYLPHESELPPADLALPWGTSADAVAGEFARQ
                     RGAATPIRLVASAKSWLCHPGVDRRAPLLPADAPPEVERISPLAASTRYLQHLRQAWD
                     AAHPGAPFDQQDITVTIPASFDPAARELTAEACRAAGYQQLTLLEEPQAALYSWIQAS
                     GGGWRKQVAHGDVILVVDVGGGTTDLSLIAVLEQGGNLQLQRVAVGEHILLGGDNMDL
                     ALAYGVARKLAAEGRQLDAWQTRALAHGCRAAKEQLLADDALQAVPVVVPSRGSKLIG
                     GSIRTEVTRAEVLALLVEGFFPPVAVSDKPQTRARAALTQLGLPYAQDAAVTRHLAAF
                     LSRQAGAVEQVEGLQGQPGASFLHPTAILFNGGVLKARQIEQRILEVVNGWLSAEGAP
                     PARLLEGANLDLAVARGAAYYGHVRRGRGVRIRGGTAQSYYVGVESNMPAIPGMEPPI
                     SALCLAPFGMEEGTEVALDAQEFGLVVGQPVRLRFFGSSMRRQDAVGSVLDFWGPEEL
                     VELQQIEAQLPAEGRTPGEVVAVQLLARVTDIGTLELLAVPVGGSERWKVEFDVRAQA
                     EEA"
     gene            complement(6881..7498)
                     /locus_tag="PK518_11945"
     CDS             complement(6881..7498)
                     /locus_tag="PK518_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HRM49671.1"
                     /translation="MSTPIHPSFLSRVAIAIGSFFAILANARLAGDIHRLRTGEPLAA
                     DVPPSEVRVEVPVEKIIETRIEVPVEKIVERRVEVATDTAALQLLGLLQREARFVDFI
                     QEDVAPYSDAEIGAAARVVHGGCRKLLAEHFTLAPVRAEAEGSRVTLAAGFDAAAVRL
                     TGNVVGQAPFTGTLSHRGWQVTQVRLPQLTDARAATIIAQAEVEL"
     gene            7687..>8149
                     /locus_tag="PK518_11950"
     CDS             7687..>8149
                     /locus_tag="PK518_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM49672.1"
                     /translation="MTIQTDDFSPAPAPRRERMVSAAPASPQEEAQERALRPKLLQEY
                     VGQAKAREQLEIFIGAARKRSEALDHVLLFGPPGLGKTTLSHIIAAELGVNLRQTSGP
                     VLEKPKDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGE"
BASE COUNT         1151 a         2887 c         2911 g         1200 t
ORIGIN      
        1 gtggcccgca ccgccgcgct gctgccatgg cggcccacgg cgcgcggcac gcaggtggcg
       61 gcgctgcgct ttgagtcgcc aggcgcgcgc ggtctgcgcc tgggcgtgct ggtgcaggcg
      121 ctgccgccgg gggcggtgct gcgcttttat gcagcagcag ccgaagacgc cgtgcagctg
      181 agcgccgagc agctgcaggc cgtggccgag cgcaacgcca gcgcgggtgc cgacgcgacg
      241 ctggcgcgca cctactggag cccggacttt ggcggcgcgc agaccacgct ggaggtcgag
      301 atacccgaag gcgcggcgcc cgccgccgtg cgcctggccg tgccgcggct gtcgcacttc
      361 acatcgacgg cggccgaggc cgaggccgcc tttaccacca aggccgcagt cgccggctgc
      421 ctggtggacg tgagctgcca ccccgattac ctggagcaaa gccgctccgt ggcgcgcatg
      481 acctatgtgg ccgaggacgg tggcgccttt tactgcaccg gcacgctgct caacgacatg
      541 gccgccagcg gcacgccgta cttcctgtcg gcccagcact gcatccccag ccaggtggta
      601 gcctcaacgc tggtgaccga ctggctgtac cgctcggcca gctgcaacag cgccgcggag
      661 aacccggcca gccggcgcct gacgggcggc gcccggctgc tgtacgcatc ggccggcacc
      721 gacaccgcct tcatgcggct gaacgacccg ctgcccgccg gcgtcgtcta cgccggctcg
      781 tactacggcg gcgtgcccat tggtacggcg ctggccagcc tgcaccacgc ggagggcggc
      841 ctgcagtcca tcagcctggg cagcgtgctg cgctacagca actgtggtga cgaaagctgc
      901 acggccacca gcggcgagac cggcaactac ctggcgctca cctggcagca gggcagcacc
      961 gagggcggta gcagcggctc gggcgtgttt gtcaccattg gcgcccggcg ctacctggcg
     1021 ggtcagctgc atggcggctc ggccagctgc gccaggcccg atggcacgga ctattacggg
     1081 cgctttgacc tgagctaccg cgctgcgctc aagaactggc tcaatccctg agggccttca
     1141 ccccaccagc accagccccg gcggcaggct gtcgcccagg ctgaggcggc ggtcctcggc
     1201 gttcatttcc tgcacctggg cgacctgggc cagccagcgc tcgggcgcgc cgctggcgcg
     1261 cagcttgtcc aggacccggg cgcgctgggc ctcgtccaga tccagcaggc gctcgcccgt
     1321 catgcgcgcg atctgcacgg cggcgaacat ggcgggctcg ttcttgcgcc agtcctgggc
     1381 cagcgtggcc tgcaagaact cttgtgcggc ctcgggcggc atgaccagat gggcgttggc
     1441 cgccagcggc gcgcgcgccg ccaggcggcc tatggcccac caggtgtggg gcggctcgtc
     1501 ggggcgcttg aggcgctcaa gcatccaccg gcccatctcc tggcggtact gccagggcac
     1561 ggcctccatc gcggcaaaca ggcgcagcat gtcgtcgtag ctgccccagc gtgacttggc
     1621 gccgcgctgc acggtctgtt gcatgcggcc ggccacgtct tcgagcacct gcatctgctg
     1681 ggcttcatcg aggcccgcgg ccacgcggcg ccacagcacc caccattcgg tccagttggc
     1741 gccctcctgg ctgtggccca ggccctgggc gtagagcggc cacagctgct gcaggcgcca
     1801 gccgtccagc tcggcgccca ggccggggcg caggcaccag ccggccaggt tgagccacac
     1861 gcgctcatgc tcggcgctgc gccggcggcg cttggcacgc gccagcaggg cgtcgaacag
     1921 cgtgcgcaag agcggcagcg gccaggattc gcgcgggccc agagtctttt ccagcgtggc
     1981 gcgcagctgg cgtacctctt tgggcgacac atcctgggtc tggttgccga agatgcggtc
     2041 gatctgggcg atggctgcgt ctagcgcgcc cctcacccct gcctctcccc agaggggcga
     2101 gggcgaccgg tcggctgggg gttttgcgcc ctctccctct gggagagggt gagggccggc
     2161 cccgcgcacg gcgaacggca gcagccagga ctggcccgcg tcctgcgtgt cgatgcagcg
     2221 cagctccagc acacccacct cgctgatgca ggcctgcagc tctacctgca cctgcgcctt
     2281 gtcgcgcccc tcgggggccg gcagcacggt ggccagcggc ggcagttcca cccagccctc
     2341 gccgtccaat gcggcgatct gcccggcctg ggcctggccg gcgctgcgcg cgaccagcgc
     2401 aaagcgcacc ggctggccca gccgcagcgc aaagcgccgg ccggccagca ccatgcgcgt
     2461 gccctcctcg gtgccgcgcg gcagcaggca gatgccctgg ggcgcggcgc cggcctggcc
     2521 cggcagcacc agccagtagc tgcgcgccgc gccgccgcct atgcgtggaa ctattgtttt
     2581 actagctgct tgcgcttgtc cagtaagcga tggtggcctt tttgactcat attccgccag
     2641 cccatgggcg gccgcgccgc gcgccacggc ccagtccggg tgcgggttgt gcagtacccg
     2701 caggggggca ccgcgccact cgcccagctg ttcggtcagg cgctgcacca gcgcatgggc
     2761 atggaacacg ccgccgttga gcagcagggt gtcgggcagt tcgcccgccg catgctgggc
     2821 caggaacagc gccagatggc gcgaaatggc cgcgtccgcc gggtagggca ggcttaaggt
     2881 ccgcagcgcg ccctggcgcc gcgcgggctg gtcggtgatc tgcgcgggcg gtaaaaagcc
     2941 ttcgaccacc gcctggcgta ccgcgtcgcg cgtcaatgtg gcggatcgtg tctgcgccag
     3001 cagttgcgag ccgccgccca gcagcgtcac ggccacggat tcgggtgcgt tggcggccag
     3061 cagctgctcc ttggccaggc ggcagcgctg caccagctgg gcaaagcgcg cggcgggcag
     3121 gcgctggcct tcgccggcaa actgcggctc caggccatgc gccagggcca gatccatgtt
     3181 gtcgccgccc agcatcaaat gctcgcccac ggccgtgcgc gtcagcagcg gcaggccgcc
     3241 gcctggtgct gcttccaccc ggatcagcgt caggtcggtg gtgccgccgc ccacgtccag
     3301 caccagcacc aggcggctgt cttgcagttg ctcggcgagt tgagagccct gcagcaccag
     3361 ccaatcgtga aacgcggcct tgggttcttc gagcagctgc acccggggca ggccggccag
     3421 ctgcgccgcc tgcagcgtga gcgcgcgcgc gccctcgtcg aaggaggcgg gcaccgtcag
     3481 caccaccagc tgctcggcca gcggcgcatc ggggtgcgtt gcatcccagg cggcgcgcac
     3541 atgcgccagg tagctgctgg aggcctgcag cggcgagacc ttggccacat cctggcccgc
     3601 gccccagggc agtatcggcg cggtgcggtc cacaccggcg tgcgacagcc agctcttggc
     3661 actggccacc agccgccccg gcacggccgc gccgaggtca cgcgcccagc ggccgatgac
     3721 ggccggggtc tgctcggttg cgtcctgcgc cggccagggc tggcgccagg catcgcccag
     3781 ctcgccgggc gcggcctggt agcgcgtgga gggcagcagt ggctgctctt gcacctcggc
     3841 cgccgagatg cgctgcgcca gcggcagcag cgcgatgtcg gccacgccgc catccagcgg
     3901 tacatgggcc accacggtgt ggctggtgcc caggtctatg ccgacggcgt ggcgcgcggc
     3961 cgctggttgg ggcggcgggg tcatggcctg cgttcagcgc ctacagcacc gtccagtcgg
     4021 tgacaaaacc gtcggggcgg cggaaggtgt cgttgagcag catgtcgggc ggcagatcca
     4081 gcgagtagcc gcgcccgggc agcggctgct caaaccattt cttgtagatc tggcgcgccg
     4141 tgccgtcgcg gtacaagtcc ttcatggcct ggttgaccag gttttgaaag tccggcgcgt
     4201 acttggacac cagcggcgcc agcggctcca ccgacaggcg cgcgcccacc accttcaccg
     4261 tgcccttgag caggcgggcc tggtcgatga gggagatgtc gtccgagaca aaggcgtcgg
     4321 cgcgcccctg ggccacggcc ttggcgcctt cctcggtgct ggccacggtg accagggtca
     4381 gccccggctg gcgctcgctg acctgctggc cggtgctgcc gttgatcacc gccacgcgcg
     4441 cctggcccat ttgcgccagt tgctggcgcg tgtcgttggc gcgcaccagc atgcgcgcgc
     4501 cggcgtagta gtaggtcagg ccaaaggcca cgcgctcgcg ccgcgccttg gtgttggtgg
     4561 agtcggcgca ttccagctgg atcttgccgt cggcgatgcg gtcaaagcgc tcggcgatcg
     4621 tcaccggcac atagtgcagc tgcagcgccg gcagcttgag ctggctttgg atgcgcgcgg
     4681 ccagctgctg gcacagctcg accgcatagc ccacgggctt gccgccctcg ccggcgtagg
     4741 acagcggggc ggcgtccagg cgatagccaa tggtgagctg gccgctggac tggatcttgg
     4801 ccagcgtggc gtcggcggcc caggtgggca gggccagggc ggcgatggcg cccgcggcca
     4861 gccagtgctt gaagaaggaa tgcatgtggt acctgcttga acggtggttc aggttgggaa
     4921 aattgctgct tcccgggtgg ggatatgccg cgggcgccgg gtttgagtag cgtgcggcag
     4981 tatcaaatgt aaccgtacgt tcaggcttcc tcggcctgtg cccgtacgtc gaactccacc
     5041 ttccagcgct cgctgccccc caccggcacg gccagcagct ccagcgtgcc gatgtcggtc
     5101 acgcgcgcga gcagttgcac ggccaccacc tcgcccggcg tgcggccttc ggcgggcagc
     5161 tgggcttcga tctgctgcag ctccaccagc tcctcggggc cccagaagtc gagcacgctg
     5221 cccacggcgt cctggcggcg catgctggag ccaaagaagc gcaggcgcac cggctggccc
     5281 accaccaggc cgaattcctg ggcgtccagc gccacctccg tgccctcctc catgccaaag
     5341 ggcgccaggc acagggcgct gatgggcggc tccataccgg ggatggcggg catgttgctc
     5401 tccacgccga cgtaataact ctgcgccgtg ccgccgcgta tgcgcacgcc gcggccgcgc
     5461 cgcacatggc cgtagtaggc ggcgccacgc gccacggcca ggtccaggtt ggcgccctcc
     5521 agcaggcgcg cgggcggcgc gccctcggcg ctgagccagc cgttcacgac ctccaggatg
     5581 cgctgctcga tctgtcgcgc cttcagcacg ccgccgttga acagaatggc cgtggggtgc
     5641 agaaagctcg cgcccggctg gccctgcagg ccctcgacct gctccacggc gccggcctgg
     5701 cgcgacagaa aggccgccag gtggcgcgtc accgccgcgt cctgcgcata gggcaggccc
     5761 agctgcgtga gcgcggcgcg ggcgcgggtc tgcggcttgt cgctgaccgc gaccgggggg
     5821 aagaagccct ccaccagcag tgccagcacc tcggcacgcg tgacctcggt gcggatgctg
     5881 ccgccgatca gtttgctgcc gcggctgggc acgaccacgg gcacggcctg cagggcgtcg
     5941 tcggccagca gctgctcctt ggcggcgcgg cagccgtgcg ccagggcgcg ggtctgccag
     6001 gcgtccagct gccggccctc ggccgccagc ttgcgcgcca cgccgtaggc cagggccaga
     6061 tccatgttgt cgccgccgag caatatgtgc tcgcccacgg ccacgcgctg caactgcagg
     6121 ttgccgccct gctccagcac ggcgatcagc gacaggtcgg tggtgccgcc gcccacgtcc
     6181 accaccagga tcacgtcgcc atgcgccacc tgcttgcgcc agccgccgcc gctggcctga
     6241 atccagctat agagcgcggc ctggggttcc tccagcaggg tcagctgctg gtagccggcg
     6301 gcgcggcagg cctcggccgt cagctcgcgc gcggccgggt cgaaggatgc gggaatggtg
     6361 acggtgatgt cctgctggtc gaacggcgcc ccggggtggg ccgcgtccca ggcctggcgc
     6421 aggtgctgca ggtagcgggt gctggcggcc aggggggata tgcgctctac ctcgggcggc
     6481 gcgtcggccg gcagcagcgg cgcgcgccta tccacgccgg ggtggcagag ccagctcttg
     6541 gcgctggcca ccaggcgtat cggcgtggcc gcgccgcgct ggcgtgcgaa ttcgccggcc
     6601 acggcgtcgg cactggtgcc ccagggcagg gccaggtcgg cggggggcag ctcgctctcg
     6661 tgcggcaggt acaaaaagga gggcagcagc gggcggcctt ccacgctggc cggggctgtg
     6721 agctggggaa cgtccagcac ctgctgcacg acctgctcgc catcgctggc ctgcatgtcc
     6781 acataggaca gggcgcaatg cgtggtgccc aggtcgattc caatggaaaa tcgggccgta
     6841 gcgcccgtgg gtaatgcgct atcagctatt gtttctatag tcacagctcc acctccgcct
     6901 gagcgatgat ggtggcggcc ctggcgtcgg tcagctgcgg caggcgcact tgggtcacct
     6961 gccagccgcg gtggctcaag gtgccggtga acggcgcctg gccgaccacg ttgcccgtca
     7021 ggcgcacggc ggcggcgtca aagccggcgg ccagcgtgac gcggctgccc tcggcctcgg
     7081 ctcgcacggg cgccaaagtg aaatgctcgg ccagcagctt gcggcagccg ccatgcacca
     7141 cgcgcgcggc ggcgccgatc tcggcgtcgc tgtagggcgc cacgtcctct tggatgaagt
     7201 ccacgaaacg cgcctcgcgc tgtagcaggc ccagcagttg cagggcggcg gtgtcggtgg
     7261 ccacctccac gcggcgttcc acgatcttct cgaccggcac ctcgatgcgg gtctcgatga
     7321 tcttttccac cggcacctcc acgcgcacct cgctgggcgg tacatctgcc gccaggggtt
     7381 cgcccgtgcg caggcggtgg atgtcgccgg ccaggcgggc gttggccagg atggcgaaaa
     7441 agctgccgat ggcgatggcc acgcggctga ggaaggatgg gtgtatcggg gtgctcatgc
     7501 ggttctcgct gcgagaaggg gacgccgggc caggggccac ggatgctgga aaagagtcac
     7561 gggcgcgcgc ccgtgaaagg gggcgatgtt acccaaacgc ggccggcagc cttctggccg
     7621 caccaggccg gcgggatgat tgggggacaa tgcacgcgcc tgatttggcg ccatttcctt
     7681 gcccgcatga ccatacaaac cgacgatttc tcccccgcac cggcgcccag gcgcgagcgc
     7741 atggtctcgg ccgcgccggc ctcgccgcag gaggaggcgc aagagcgcgc cctgcgcccc
     7801 aagctgctgc aggaatacgt gggccaggcc aaggcgcgcg agcagctgga gatcttcata
     7861 ggcgccgcca ggaagcgcag cgaggcgctg gaccatgtgc tgctgttcgg cccgcccggt
     7921 ttaggaaaaa ccacgctcag ccacatcatc gccgccgagc tcggcgtgaa cctgcgccag
     7981 accagcggcc cggtgctgga aaagcccaag gatctggcgg cgctgctgac caacctggag
     8041 aagaacgacg tcctgttcat cgacgagatc caccgcctgt ccccggtggt ggaagaaatc
     8101 ctctaccccg cgctggagga ctaccagatc gacatcatga tcggcgagg
//
LOCUS       DAOMTQ010000132         8133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1962_length_8133_cov_28.574523, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000132 DAOMTQ010000000
VERSION     DAOMTQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1962_length_8133_cov_28.574523"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /gene="trkA"
                     /locus_tag="PK518_11955"
     CDS             <1..980
                     /gene="trkA"
                     /locus_tag="PK518_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRM49673.1"
                     /translation="MPEVAVRIVAIYRRFPDEPDRFVPCDGTTRIEPGDEVFALAGMQ
                     RVRQVLAALRLRAGQPPRVVKRIMMAGGGVVSERLARLLAEQPERFHIKIVEPGQERS
                     VALASSLPEQVLVLQGETSDEELLAEEGIEEVDFFLALSDDDENNIMSCLLAKRMGAR
                     RVLALIHRQAYAELMHGTQIDIALSPAQAMLGELLAYTRRGDVQAVHSLRRGVAEALE
                     IVARGDRKSSRVVGRRVEELPLPPQVHMGLIVRGLPDADAPPPADGEGEGEGEGEGEA
                     PPAAPAEPEVIIPRSHTVIESNDHVVFFLPNKRLVREVEKLFRVSATFF"
     gene            991..2457
                     /locus_tag="PK518_11960"
     CDS             991..2457
                     /locus_tag="PK518_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRM49674.1"
                     /translation="MRDLFPVLRVLGMLMCMFALAMALPGAVSWWQQESVWRVYPLSM
                     AVTMGAGLLLWWLLRHHRQELQPRHGVILVTLVWVLLPLAASLPLWLAMAQVARPISF
                     THAYFEAVSGLTTTGSTVLSGLDALPLSVNIWRTFLQWMGGMGILILAVAVLPLLGVG
                     GSQLFKAEAAGPIKDTKLTPRMTETAKGLWGVYAGFSVLCAIAFWAAGMSPMDALMHM
                     FTTVSLGGLSSHDSSFAFFDSPLLEAVAVFFMLVASCNFALYFVALRKGHWQSFWRDP
                     EVRATLVALVGGGLLVALLLWAKGMYGPLTALRHGMFHVVSVATTTGYATVDYLAWPV
                     FAPVFMLLLSGVATSAGSTGGGIKMVRMLILVQQARRELTRLVHPRAVQPVLLGGRVV
                     DSRMIFSVMAFMLVYGATVIVLSMVLLLTDLDAVTAFSAVLASVHCTGPGLGMIGPSS
                     NYAQLTDFQVWVCTLGMLLGRLEILSFMALLSPAFWRR"
     gene            2525..3820
                     /gene="gshA"
                     /locus_tag="PK518_11965"
     CDS             2525..3820
                     /gene="gshA"
                     /locus_tag="PK518_11965"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRM49675.1"
                     /translation="MVPHLVTALTGPINELEQRILDSMPAIERWFRLEWMEHTPPFYT
                     SVDIRNAGFKLAPVDTNLFPGGWNNLTNEMLPLAVQAAMAAIEKICPEARNLLIIPEN
                     HSRNTFYLANVAQLMRIFNMAGLNVRVGSISADIKKSTTINLPHGESVTLEPVIRNKR
                     RLGLKNFDPCTILLNNDLSAGAPGILEELYEQYLLPPLHAGWSVRRKSHHFKSYEEVT
                     KRFGKMLGIDPWLINPLFGHCEGVDFNEGTGLDGLRATVDAVLAKVRRKYKEYGIKEK
                     PFVVVKPDNGTYGMGIMTVHDAKELDDLSRKTRNKMAVIKDGQVVHDVIVQEGVLTQE
                     RVHEAVAEPVVYMMDRYVVGGFYRINSERGVDENLNAPGASFVPLAFEHSTHLPQPGA
                     RPGVSAPNRFYMYGVIARLAMLAASYELEATDPEAEVYD"
     gene            3938..5806
                     /locus_tag="PK518_11970"
     CDS             3938..5806
                     /locus_tag="PK518_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRM49676.1"
                     /translation="MQNSKSSLAGLTVGAIGVVFGDIGTSVLYTVKEVFATGHVQFTH
                     DNVYGVLSLIFWTLTIIVSVKYVVLVLRADNNGEGGLVAMLTLASRAVADRPRLRHWM
                     LLVGIFGTCLFYGDGVITPAITVLGAMEGLEVMSPELTKYVIPLTLLVLFLLFLFQKQ
                     GTAGIGKFFGPVMLLWFATIAALGLLHIARHPEILWAVSPHYAWVFALHNPGITFIIL
                     GAVVLCVTGGEALYADMGHFGKRPIRLAWFSIAMPALVLNYFGQGALLLAEPEAVKNP
                     FFNLAPEWATLPLVCLATAAAVIASQALISGAFSVTKQVIQLGFLPRLQIQHTSVHDK
                     GQIYMPFVNWGLFALIVLAVGMFRSSGNLAAAYGIAVTTDMLITTVLTFFVVRYAWKL
                     PLLLCIGATGLFFTVDILFWSSNLLKLIHGGWFPLVIAGAVFLLMVTWRDGRALLTQA
                     LQDQALDLKDFLSALFIAPPTRVEGAAVFLTSRDGIVPNALLHNLKHNKVLHGQNLFV
                     TVQNHEVPWIGMEKRLEAEALGNDCWRVIVNYGFKNDYDLPKALSLLREQGVDLNPMT
                     TSYFLSRDIVTPTLGVGMALWREKLFAQMHHSASAAAEFLNLPNNAVVELGSKVEI"
     gene            5892..7094
                     /locus_tag="PK518_11975"
     CDS             5892..7094
                     /locus_tag="PK518_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRM49677.1"
                     /translation="MPQSVAMQFFKDLSLSAATAGFVAVLVGFTSSVAIVFQAAQAFG
                     ATPGQITSWMWALGLGMGLCSLVPSLILRQPVMVAWSTPGAAVLATAGLAGGFSMGEA
                     IGAFMVCALLITLAGVTGWFERVMNRIPMEIASALLAGVLARFGMQAFAALQTALPLV
                     LVMLAAYLLARRLLPRYAVVLTLAVGIAWAAVDGQMRWSEVHFALAVPVFTAPSFSLQ
                     ALMSLALPLFIVTMASQNLPGVAVMRATGYQLPVSRLISMTGLVTLLLAPFGAFALNF
                     SAITAAICMGPEAHPDPARRYTAAASCGLLYIAVGIFGAVVTGLLTAFPAELVVAIAG
                     LALLGAIGGGLASALADEGHREAALITFLVTLSGVSLAGIGSAFWGVVAGSAALFVQQ
                     YGRRGAHH"
     gene            7122..8075
                     /gene="gshB"
                     /locus_tag="PK518_11980"
     CDS             7122..8075
                     /gene="gshB"
                     /locus_tag="PK518_11980"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRM49678.1"
                     /translation="MNLLFIADPVEHFKIYKDTTFAMMREAQRRGHALAVSEPRHMHW
                     QRGGRVVARVRDITLTGHAQDWFIAGEPREVALAEYDAVLMRKDPPFDSEYFYATHLL
                     EQAEREGARVFNKPRALRDHPEKLAILEFPQFIGPTLVTRDAQDIRRFHAEHGDIILK
                     PLDGMGGMGIFRVGPDGMNLGSIIETLNRDGSQSVMVQKFLPEITEGDKRVLVIGGKP
                     VPYCLARIPQGTEVRGNLAAGGLGVARALSVRDREIGEALGPLLQQRGLLLAGVDVIG
                     DCITEINVTSPTCFQEIFDQTGCDVAALFVDALEAAVAQAS"
BASE COUNT         1203 a         2617 c         2808 g         1505 t
ORIGIN      
        1 agatgcccga ggtggcggtg cgcatcgtcg ccatctaccg gcgctttccc gacgagcccg
       61 accgcttcgt gccctgcgac ggcaccacgc gcatagagcc cggcgacgag gtgttcgcgc
      121 tggcggggat gcagcgcgtg cgccaggtgc tggccgctct gcgcctgcgt gcgggccagc
      181 cgccgcgcgt ggtcaagcgc atcatgatgg ccggtggcgg cgtggtcagc gagcgcctgg
      241 cgcgcctgct ggccgagcag ccggagcgct ttcacatcaa gatcgtcgag ccgggccagg
      301 agcgcagcgt ggcgctggct tccagcctgc ccgaacaggt gctggtgctg cagggcgaga
      361 cctcggacga ggagctgctg gccgaggagg gcatcgagga ggtggacttc ttcctggccc
      421 tgtcggacga cgacgagaac aacatcatgt cctgcctgct ggccaagcgc atgggcgcgc
      481 gccgcgtgct ggcgctgatc caccgccagg cctatgccga gctgatgcac ggcacgcaga
      541 tcgacattgc gctgtcgccg gcccaggcca tgctgggcga gctgctggcc tacacgcgcc
      601 gtggcgacgt gcaggccgtg cacagcctgc gccgcggcgt ggccgaggcg ctggagatcg
      661 tggcgcgcgg cgacaggaag agctcgcgcg tggtcggccg gcgcgtggaa gagctgccgc
      721 tgccgcccca ggtgcacatg ggcctgatcg tgcgcggcct gcccgatgcc gacgcgccgc
      781 caccggccga tggcgagggt gagggtgagg gcgagggcga gggcgaggca ccaccggccg
      841 cgccggcgga gcccgaggtg atcattccgc gcagccatac ggtcatcgag agcaacgacc
      901 atgtggtgtt ctttctgccg aacaagcgcc tggtgcgcga ggtggaaaag ctgttccgcg
      961 tgagcgccac gttcttttga gccctcggcc atgcgtgacc tgttccccgt tctgcgtgtg
     1021 ctgggcatgc tgatgtgcat gtttgcgctg gccatggcgc tgcccggcgc cgtgtcgtgg
     1081 tggcagcagg agagcgtgtg gcgcgtctac ccgctgtcca tggccgtgac catgggcgcg
     1141 ggcctgctgc tctggtggct gctgcgccac caccgccagg agctgcagcc gcgccatggc
     1201 gtgatcctgg tgacgctggt gtgggtgctg ctgccgctgg cggccagcct gccgctgtgg
     1261 ctggccatgg cgcaggtggc gcggccgatc agcttcacgc atgcctattt cgaggccgtc
     1321 tcgggcctga ccaccaccgg ctccacggtg ctgtcggggc tggatgcgct gccgctgtcg
     1381 gtcaatatct ggcgcacctt tttgcagtgg atgggcggca tgggcatcct gatcctggcc
     1441 gtggccgtgc tgccgctgct gggcgtgggc ggcagccagc tgttcaaggc cgaggccgcc
     1501 ggccccatca aggacaccaa gctgaccccg cgcatgaccg agacggccaa ggggctgtgg
     1561 ggtgtgtatg cggggttttc ggtgctgtgc gccatcgcct tctgggcggc gggcatgtca
     1621 cccatggatg cgctgatgca tatgttcacc accgtcagcc tgggcgggct gtcctcgcac
     1681 gacagcagct ttgccttctt tgactcgccc ctgctggagg ccgtggcggt gttcttcatg
     1741 ctggtggcca gctgcaactt tgcgctgtac ttcgtggcgc tgcgcaaggg gcattggcag
     1801 tcgttctggc gcgaccccga ggtgcgcgcc acgctggtgg cgctggtcgg cggcggcctg
     1861 ctggtggcgc tgctgctctg ggccaagggg atgtacgggc cgttgacggc gctgcgccac
     1921 ggcatgttcc atgtggtgtc ggtggccacc accaccggct atgccacggt ggactacctg
     1981 gcctggccgg tgttcgcgcc ggtgttcatg ctgctcttgt cgggcgtggc caccagcgcc
     2041 ggatcgaccg gcggcggcat caagatggtg cgcatgctga tcctggtgca gcaggcgcgg
     2101 cgcgagctca cgcgcctggt gcacccgcgc gccgtgcagc cggtgctgct gggcggcagg
     2161 gtggtggaca gccgcatgat cttctcggtg atggccttca tgctggtcta cggcgccacg
     2221 gtgatcgtgc tgagcatggt gctgctgctg accgatctgg atgcggtgac ggcgttttcg
     2281 gccgtgctgg ccagcgtgca ctgcaccggg ccggggctgg gcatgattgg gcccagctcc
     2341 aactacgcgc agctgaccga cttccaggtc tgggtctgca cgctgggcat gctgctcggg
     2401 cggctggaga ttctgagctt catggcgctg ctgtcccccg cgttctggcg tcgttgaact
     2461 acgtatgcgc ccgcctgggg cgcgtcccta caatgctgcg cttgactgca ttacatgggg
     2521 ttatatggtt ccgcaccttg ttaccgcact cacgggcccg atcaacgagc tggagcagcg
     2581 cattctcgac tccatgccgg ccatcgagcg ctggtttcgc ctggagtgga tggagcacac
     2641 gccgccgttc tacacctcgg tggacatccg caacgccggc ttcaagctgg cgccggtgga
     2701 taccaacctg tttcccggcg gctggaacaa tctgaccaac gagatgctgc cgctggcggt
     2761 gcaggcggcc atggcggcca tcgagaagat ctgccccgag gcgcgcaacc tgctcatcat
     2821 cccggagaac cattcgcgca acaccttcta cctggccaac gttgctcagc tgatgcgcat
     2881 cttcaacatg gcggggctga acgtgcgcgt gggctcgatc agcgctgaca tcaagaagag
     2941 caccaccatc aacctgccgc atggcgagtc ggtgacgctg gagccggtga tacgcaacaa
     3001 gcgccgcctg gggctgaaga acttcgaccc ctgcaccatc ttgctgaaca acgacctgtc
     3061 ggccggtgcg ccgggcatcc tcgaagagct gtacgagcaa tacctgttgc cgccgctgca
     3121 cgcgggctgg agcgtgcgca gaaagagtca ccacttcaag agctacgagg aggtgaccaa
     3181 gcgctttggc aagatgctgg gcatagaccc ctggctcatc aacccgctgt tcggtcattg
     3241 cgagggcgtg gacttcaatg agggcaccgg cctggacggc ctgcgcgcca cggtggatgc
     3301 ggtgctggcc aaggtgcggc gcaagtacaa ggaatacggc atcaaggaaa agcccttcgt
     3361 cgtggtgaag cccgacaacg gcacctatgg catgggcatc atgacggtgc acgacgccaa
     3421 ggagctggac gatctgagcc gcaagacgcg caacaagatg gccgtgatca aggacggcca
     3481 ggtggtgcat gacgtgatcg tgcaggaggg cgtgctgacg caggagcgcg tgcacgaggc
     3541 cgtcgccgag cccgtggtct acatgatgga tcgctatgtg gtcgggggct tttatcgcat
     3601 caattccgag cgcggcgtgg acgagaacct gaacgccccc ggcgccagct ttgtgccgct
     3661 ggccttcgag cacagcaccc atctgccgca gcccggtgcg cgcccgggtg tcagcgcgcc
     3721 gaaccgcttc tacatgtacg gcgtgatcgc acgcctggcc atgctggcgg ccagctacga
     3781 gctggaggcc accgacccgg aggccgaggt ctacgactga gcccggttca gcgcccgtgg
     3841 cagccctgcc acgggcttgg tgcgcggcgc ctgcggcggg cgcacaatca gcatcccttc
     3901 ctaccaggaa ccccggccgc gcgccgggag acagtccgtg caaaactcga aatcctcgct
     3961 cgcgggtctg accgtgggcg ccatcggcgt ggtcttcggc gacatcggca ccagcgtgct
     4021 ctacaccgtc aaggaggtgt tcgccaccgg ccatgtgcag ttcacccatg acaacgtgta
     4081 tggcgtgctg tcgctgatct tctggacgct caccatcatc gtctccgtca aatatgtggt
     4141 gctggtgctg cgcgccgaca acaacggcga gggcggcctg gtggccatgc tcacgctggc
     4201 gtcgcgcgcc gtggcggatc ggccccggct gcgccactgg atgctgctgg tgggcatttt
     4261 tggcacctgc ctgttctatg gcgacggtgt catcaccccc gcgatcacgg tgctgggcgc
     4321 catggagggg ctggaggtga tgtcgcccga gctgaccaag tacgtcatcc cgctgacgct
     4381 gctggtgttg tttctgctgt ttttgttcca gaagcagggc acggccggca taggcaagtt
     4441 cttcggcccg gtgatgctgc tgtggtttgc caccatcgcg gcgctggggc tgctgcacat
     4501 cgcccggcac ccggagattc tgtgggccgt ctcgccgcat tacgcctggg tgttcgcgct
     4561 gcacaacccg ggcatcacct tcatcatcct gggcgcggtg gtgctgtgcg tgaccggcgg
     4621 cgaggcgctg tacgccgaca tggggcattt tggcaagcgc ccgattcgcc tggcctggtt
     4681 ctccatcgcc atgccggcgc tggtgttgaa ctactttggc cagggcgcgc tgctgctggc
     4741 cgagcccgag gcggtgaaga acccgttctt caacctggcg cccgaatggg ccacgctgcc
     4801 gctggtctgc ctggccacgg cggcggcggt gatcgcctcg caggcgctga tctcgggcgc
     4861 tttcagcgtc accaagcagg tgatacagct cgggtttctg ccgcgcctgc agatccagca
     4921 caccagcgtg catgacaagg ggcagatcta catgcccttt gtcaactggg ggctgttcgc
     4981 gctcatcgtg ctggcggtgg gcatgttccg cagctcgggc aacctggcgg cggcctacgg
     5041 cattgcggtg accaccgaca tgctgatcac caccgtgctc accttcttcg tcgtgcgcta
     5101 tgcctggaag ctgccgctgt tgctgtgcat aggggcgacg ggcctgttct tcacggtgga
     5161 catcctgttc tggtcgtcga atctgctcaa gctgatccac ggcggctggt tcccgctggt
     5221 gatcgccggg gcggtgttcc tgctgatggt gacctggcgc gacggccgcg cgctgctgac
     5281 ccaggcgctg caggaccagg cgctggatct gaaggatttt ctgtcggcgc tgttcatcgc
     5341 cccgcccacg cgcgtcgagg gcgcggcggt gttccttacc tcgcgcgacg gcatcgtgcc
     5401 caatgccctg ctgcacaacc tcaagcacaa caaggtgctg cacgggcaaa acctgttcgt
     5461 caccgtgcag aaccacgagg tgccctggat aggcatggaa aagcggctgg aggccgaggc
     5521 gctgggcaat gactgctggc gcgtgatcgt caattacggc ttcaagaacg actacgacct
     5581 gcccaaggcg ctgtccctgc tgcgggagca gggcgtggat ctgaacccga tgacgaccag
     5641 ctacttcctc tcgcgcgaca tcgtcacgcc cacgctgggt gtgggcatgg cgctgtggcg
     5701 tgaaaagctg tttgcccaga tgcaccacag cgccagcgcg gcggcggagt tcctgaatct
     5761 gcccaacaac gcggtggtgg agctgggctc caaggtggag atttgaagtg tttttggccg
     5821 tttgcacatg ctggtaaagc gcaaacagct atcaaatata tagtgaatgc cgctgcgcga
     5881 taccggcggc gctgccacaa tccgtcgcca tgcagttctt caaagacctg agcctgtcgg
     5941 cagccaccgc gggctttgtg gccgtgctgg tgggtttcac cagctcggtg gccatcgtgt
     6001 tccaggcggc ccaggcgttt ggcgccacgc cggggcagat cacctcgtgg atgtgggccc
     6061 tgggcctggg catgggcctg tgctcgctgg tgccctcgct catcctgcgc cagcccgtca
     6121 tggtggcctg gtccacaccg ggcgccgcgg tactggccac ggcggggctg gcggggggct
     6181 tttcgatggg cgaggccata ggcgccttca tggtctgcgc gctgttgatc acgctggcgg
     6241 gggtgaccgg ctggttcgag cgcgtgatga accgcattcc catggagatc gcctcggccc
     6301 tgctggcggg cgtgctggcg cgctttggca tgcaggcctt tgcggcgctg cagacggcgc
     6361 tgccgctggt gctggtcatg ctggccgcct acctgctggc gcgtcgcctg ctgccgcgct
     6421 atgcggtggt gctcacgctg gccgtgggca tcgcctgggc cgcggtcgat gggcagatgc
     6481 gctggtccga ggtgcatttc gcgctggccg ttcccgtctt taccgcgccc agcttcagcc
     6541 tgcaggcgct gatgagcctg gccctgccgc tgttcatcgt caccatggca tcgcaaaacc
     6601 tgcccggcgt ggccgtgatg cgcgccacgg gctaccagct gccggtctcg cggctgatca
     6661 gcatgacggg ccttgtcacg ctgctgctgg cgccctttgg cgcgtttgcg ctgaacttca
     6721 gcgccatcac cgccgccatc tgcatggggc ccgaggcgca cccggatccg gcacggcgct
     6781 acaccgccgc ggcctcctgc gggctgctgt acatcgccgt cggcatcttc ggcgcagtgg
     6841 tcacggggct gctgacagcc ttccctgcgg agctggtggt ggccattgcc gggctggcgc
     6901 tgctgggcgc catcggcggt ggcctggcat cggcgctggc cgatgagggc caccgcgagg
     6961 ccgccctcat caccttcctg gtcaccctca gcggcgtgag cctagccggc ataggctcgg
     7021 cattctgggg cgtggtcgcc ggcagcgcgg cgctgtttgt gcaacagtat gggcgccgcg
     7081 gcgcgcacca ttgacgccgg ccgctgaagc cacagcctgc catgaacctc ctcttcatcg
     7141 ccgaccccgt cgagcatttc aagatctaca aggacaccac cttcgccatg atgcgtgagg
     7201 cccagcggcg cggccacgcg ctggccgtga gcgagccgcg ccacatgcac tggcagcgcg
     7261 gcggcagggt ggtggcgcgc gtgcgcgaca tcacgctcac cggccatgcg caggactggt
     7321 tcattgccgg cgagccacgc gaagtggcgc tggccgagta cgacgccgtg ctcatgcgca
     7381 aggatccgcc cttcgacagc gagtatttct acgccaccca cctgctggag caggccgagc
     7441 gcgagggcgc ccgcgtgttc aacaagccca gggcgctgcg cgaccacccg gagaagctgg
     7501 ccattctgga gttcccgcaa ttcatcggcc ccacgctggt gacgcgcgac gcgcaggaca
     7561 ttcggcgctt tcacgccgag catggagaca tcatcctcaa gcccctggac ggcatgggcg
     7621 gcatgggcat cttccgtgtc ggcccagatg gcatgaacct gggcagcatc atcgaaaccc
     7681 tgaaccggga cggcagccag agcgtcatgg tgcaaaagtt tctgcccgag atcaccgaag
     7741 gggacaagcg tgtgctcgtc atcggcggaa agcccgtgcc gtactgcctg gcacgtattc
     7801 cgcagggcac cgaggtgcgc ggcaacctgg ccgcaggtgg gctgggcgtg gcgcgcgcgc
     7861 tctcggtacg cgatcgcgag atcggcgagg ccctgggccc gctgctgcag cagcgcggct
     7921 tgctgctggc cggcgtggat gtcattggcg attgcattac cgagatcaac gtcaccagcc
     7981 cgacatgctt tcaggaaatc tttgaccaga ccggctgcga tgtggctgcg ctattcgtcg
     8041 atgcgctgga agcggccgtc gcacaggcca gctgaaatct ttccgtacgg gccttttttg
     8101 cggctatact agcgggctcg ctgcagcgaa agt
//
LOCUS       DAOMTQ010000133         8038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_1995_length_8038_cov_26.275586, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000133 DAOMTQ010000000
VERSION     DAOMTQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1995_length_8038_cov_26.275586"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PK518_11985"
     CDS             complement(<1..829)
                     /locus_tag="PK518_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM49679.1"
                     /translation="MDLALERIHKKVGAQTWLYELDMALQSNAVTVLLGATQAGKTSL
                     MRIMAGLDAPTGGRVRVDGRDVTGMPVRERNVAMVYQQFINYPSMSVADNIASPLKLR
                     GEKNVAARVRELAERLHIDMFLDRLPAELSGGQQQRVALARALAKGAPLMLLDEPLVN
                     LDYKLREELREELAQLFAAGQSTVVYATTEPGEALLLGGYTAVLDEGRLLQYGPTAEV
                     FHAPCSLRVARAFSDPPINLVPAVATAAGLRLQGGLELAPRLPLVALATKKVLGEDLL
                     "
     gene            1017..1784
                     /locus_tag="PK518_11990"
     CDS             1017..1784
                     /locus_tag="PK518_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM49680.1"
                     /translation="MNTNPRQIQLLEELRERKSATVEQLAETLGVTLQTVRRDVQRLA
                     EQGLLSRFHGGVRLPSSTVENIAHNQRETLNSEGKARIARAVAAQVPHHCSLILNIGT
                     TTEAIAKALLRHQGLRVITNNLNVAAILCGNPDCEVIVAGGVVRTRDGGIVGEAAVDF
                     IRQFRVDIAIIGISGIEDDGTLRDFDYREVKVAQTIVEHAREVWLAADHSKFNRPAMV
                     QLATLDQIDRLFTDAPPPEPFPALLGNADVQLVVATD"
     gene            1806..3302
                     /gene="glpK"
                     /locus_tag="PK518_11995"
     CDS             1806..3302
                     /gene="glpK"
                     /locus_tag="PK518_11995"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRM49681.1"
                     /translation="MTYLLALDQGTSSSRSIVFDERGRIVAQAQRELPQIYPQPGWVE
                     HDPLEIWRTQLATAREALAQAGIGASSVRALGITNQRETTVLWNRKTGQPVHHAIVWQ
                     DRRAEPACAQLRAQGHAAAIQAKTGLLVDAYFSGTKLQWLLDHVSGARAAAERGELAF
                     GTVDSWLIWQLTGGQRHVTDVSNASRTMLLNVHTNQWDEELLDLLRIPRSLLPEVLPS
                     ASDFGRTQADLLGGPIAIGGVAGDQQSALFGQACFSAGMAKNTYGTGCFMLMHTGSQF
                     QSSHNGLITTSAAQPTAQPAFALEGSVFVGGAVVQWLRDGLRAISASSEVQALAESVP
                     DSGGVMLVPAFTGLGAPYWNPDARGTITGLTRGSTLAHIARAALESIAYQSAALLSAM
                     SRDAVANGGAPVSELRVDGGACVNDLLMQFQADLLGIPVVRPAVVETTALGAAYLAGL
                     SSGVYASQDELTTLWQAERRFMPTLAREQAQALMARWEHAVAQAVLPA"
     gene            3460..4296
                     /gene="proC"
                     /locus_tag="PK518_12000"
     CDS             3460..4296
                     /gene="proC"
                     /locus_tag="PK518_12000"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRM49682.1"
                     /translation="MSHSHQPLPPIAFIGGGNMASAIIGGLIQQGLPASQIEVVEPFE
                     PARAALLQGFGVSAQPTATAALARAALVVWAVKPQTFKDAAQQAAPHTQGALHLSVAA
                     GIRSGSIAQWLSSERIVRAMPNTPALIGQGMTGLYARAAVTADERTLVEQVVATTGAW
                     LWLEQESLLDAVTALSGSGPAYVFYFLEAMTEAGEDMGLTREQAHQLAVATFAGASEL
                     ARRSDEPPAVLRQRVTSKGGTTHAAIESMEADDMAQRFMRALDAAEHRARELGDEFGA
                     TN"
     gene            4611..>5402
                     /locus_tag="PK518_12005"
     CDS             4611..>5402
                     /locus_tag="PK518_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRM49683.1"
                     /translation="MDKNTCAQLASPPPSYPGGMVTLDTVNLDENKADDCEPEIKVLA
                     TSGDGLLKGAAAKMRLPGSSTRLATLKSYRVKFDKGADWQGEDTLNLNKHPYDLTRVR
                     NKLAFDLMRQVPYHQSLRTQFVEIRYDAGAGTGAGAQPMGLFTHIEKLGGKSYLERRG
                     WVAGSNVYKVAAFNFNPDARLQTLADGKAGPGFEELLEIENDSGNHGAIVQTVKALAD
                     DGTPFATSFNRHFDRNNYLAWLASAILLGNWDTVNQNFGLYQPLGG"
     assembly_gap    5403..5502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5503..5720
                     /locus_tag="PK518_12010"
     CDS             <5503..5720
                     /locus_tag="PK518_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore coat protein CotH"
                     /protein_id="HRM49684.1"
                     /translation="AFADGTLVLQPAPVSGRTLLNLGALPAGQYLVRVTASDADGNRS
                     NAFDSTSFDGQTVDGAICLNMPGATPC"
     gene            complement(5727..6599)
                     /gene="ubiA"
                     /locus_tag="PK518_12015"
     CDS             complement(5727..6599)
                     /gene="ubiA"
                     /locus_tag="PK518_12015"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRM49685.1"
                     /translation="MTATPPRSRLSLYLDLIRFNRPAGWLVLVWPTLVALWVAAGGFP
                     GWHLLTVFVAGTVLMRSAGCCINDIADRGFDRHVKRTQARPITSGLVSVTEAALLGAA
                     LALVALALVLTTRWQAVAWSVPAVVFTILYPFTKRFFAMPQAFLGIAFNFGIVIAFAA
                     VTGGVPGQAWVLWLANMFLVLAYDTEYAMVDRDDDLLIGMKTSAITLGRFDVAAIMLF
                     FVACLALTAAVLVPLRLGWPLWLGLGLAAAQVLWHYRLIRTRTRAGCFVAFSKSHWIG
                     AAIFAGVALGFALR"
     gene            complement(6659..7198)
                     /locus_tag="PK518_12020"
     CDS             complement(6659..7198)
                     /locus_tag="PK518_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HRM49686.1"
                     /translation="MAKAAKTSKTVKTPASAQGIAAPGIRIGISDKDRAAIAQGLSRL
                     LADTYTLYLTTHNFHWNVTGTMFNTLHTMFMEQYTELWNAVDPIAERIRALGHVAPGS
                     YAAFGKLSSLDDVPADPPKALEMVRILVLGHEAVARTARELFPLSDKACDEPTADLLT
                     QRMTVHEQTAWMLRSLLED"
     gene            complement(7298..>8038)
                     /locus_tag="PK518_12025"
     CDS             complement(7298..>8038)
                     /locus_tag="PK518_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM49687.1"
                     /translation="AEIKEIAKRGKDPLAGALTLGVIYTIGPYLLPDLVRHAIALTPQ
                     MPLMLQENFTVKLLEMLRTGEIDCAILAEPFPDTGLAIAPLYDEPFLAAVPSSHPLAE
                     RKNVSALELKNETMLLLGAGHCFRDHVLEVCPEFARFASNAEGIRRTFEGSSLETIKH
                     MVSAGMGVTLVPRLSVPRDALHTGVRRRKSDDAHIRYLPIKESDGSAPPMRRVVLAWR
                     RSFTRYEAIAALRNAIYACELPGVKRLS"
BASE COUNT         1315 a         2840 c         2619 g         1164 t
ORIGIN      
        1 ccagcaggtc ttcgcccagc accttcttgg ttgccagcgc caccagcggc aggcgcggcg
       61 ccagctccag gccgccctgc aggcgcaggc ccgcggcggt ggccacggcc ggcaccaggt
      121 tgatgggcgg gtcgctgaag gcgcgcgcca cgcgcagcga gcacggtgcg tggaacacct
      181 cggccgtggg gccgtactgc agcaggcgcc cctcgtcgag cacggcggtg tagccgccca
      241 gcagcagcgc ctcgcccggc tcggtggtgg cgtagaccac ggtggactgg cccgcggcga
      301 acagttgtgc gagctcctcg cgcagctcct cgcgcagctt gtagtccagg ttcaccagcg
      361 gctcgtcgag cagcatcagc ggcgcgccct tggccagcgc acgcgccagc gccacgcgct
      421 gctgctggcc gcccgagagc tcggccggca ggcggtcgag gaacatgtcg atgtgcaggc
      481 gctcggcgag ttcgcgcacg cgcgccgcca cgttcttctc gccgcgcagc ttgagcggcg
      541 aggcgatgtt gtcggccacg ctcatcgagg ggtagttgat gaactgctgg tacaccatgg
      601 ccacgttgcg ctcgcgcacc ggcatgccgg tgacgtcgcg cccgtccacg cgcacccggc
      661 cgcccgtggg cgcatccagc ccggccatga tgcgcatcag actggtcttg ccggcctgcg
      721 tggcgcccag cagcacggtc acggcattgc tctgcaaggc catgtccagc tcgtacaacc
      781 aggtctgcgc acccaccttc ttgtggatgc gctccaatgc caaatccatg tgcttacccc
      841 aggcggcggg ccgccgcggc cccgtcctgg gccatgggca gacctgggac agtgcgttga
      901 tgttcgtatt ttttcattct tgttcttttt tgcgcgtatt gccctatggt tttcccttaa
      961 ttttttcttt ttttttcgtt ttacagtagc aggatcacca ccccctcccc ctcagcgtga
     1021 acaccaatcc ccgccagatc cagttgctgg aagagctgcg cgagcgcaag tcggccacgg
     1081 tcgagcaatt ggccgagacc ctgggcgtga ccctgcagac cgtgcggcgc gacgtgcagc
     1141 gcctggccga gcagggcctg ctctcgcgct ttcatggcgg cgtgcgcctg cccagctcca
     1201 ccgtggagaa catcgcccac aaccagcgcg agacgctcaa ctccgagggc aaggcgcgca
     1261 tcgcgcgcgc cgtggccgcg caggtgccgc accactgctc gctgatcctg aacatcggca
     1321 ccacgaccga ggccatcgcc aaggcgctgc tgcgccacca gggcctgcgc gtgatcacca
     1381 acaacctcaa cgtggcggcc atcctgtgcg gcaacccgga ctgcgaggtc atcgtcgccg
     1441 gcggcgtggt gcgcacgcgc gacggcggca tcgtgggcga ggcggcggtg gacttcatcc
     1501 gccagttccg cgtggacatc gccatcatcg gcatctcggg catcgaggac gacggcacgc
     1561 tgcgcgactt cgactaccgc gaggtgaagg tggcgcagac catcgtggag cacgcgcgcg
     1621 aggtctggct cgccgccgac cacagcaagt tcaaccgccc ggccatggtg caattggcca
     1681 cgctcgacca gatcgaccgc ctgttcaccg acgcgccgcc gcccgagccc ttccccgccc
     1741 tgctcggaaa cgccgacgtg caactggtcg tggccaccga ctgacccttt tccccacgca
     1801 ctcccatgac ctacctgctt gcactcgacc agggcacctc cagctcgcgc agcatcgtgt
     1861 tcgacgagcg cgggcgcatc gtcgcgcagg cccagcgcga gctgccccag atctacccgc
     1921 agcccggctg ggtggagcac gaccccctgg agatctggcg cacgcagctc gccacggcgc
     1981 gcgaggccct ggcgcaggcc ggcataggcg ccagcagcgt gcgcgccctg ggcatcacca
     2041 accagcgcga aaccaccgtg ctgtggaacc gcaagaccgg ccagcccgtg caccacgcca
     2101 tcgtctggca ggaccggcgc gccgagcccg cctgcgcgca gttgcgcgcc cagggccatg
     2161 ccgccgccat ccaggccaag accgggctgc tggtggacgc ctacttctcc ggcaccaagc
     2221 tgcagtggct gctggaccac gtgagcggcg cgcgcgcggc ggccgagcgc ggcgagctgg
     2281 ccttcggcac ggtcgacagc tggctcatct ggcagctcac gggcggccag cgccatgtga
     2341 cggacgtgag caacgccagc cgcaccatgc tgctgaacgt gcacaccaac cagtgggacg
     2401 aggagctgct ggacctgctg cgcattccgc gttcgctgct gcccgaggtg ctgccctcgg
     2461 ccagcgactt cggccgcacc caggccgacc tgctgggcgg ccccattgcc atcggcggcg
     2521 tggcgggcga ccagcaaagc gccctgttcg gccaggcctg cttttccgcc ggcatggcca
     2581 agaacaccta cggcacgggc tgcttcatgc tcatgcacac gggcagccag ttccagagca
     2641 gccacaacgg cctgatcacc accagcgccg cccagcccac ggcccagccg gcctttgcgc
     2701 tggagggcag cgtgttcgtc ggcggcgccg tggtgcagtg gctgcgcgac gggctgcgcg
     2761 ccatctcggc cagctccgag gtgcaggcgc tggccgagag cgtacccgac tcgggcggcg
     2821 tgatgctggt gccggccttc accggcctgg gcgcgcccta ctggaacccg gacgcgcgcg
     2881 gcaccatcac cggcctgacg cgcggcagca cgctggcgca catcgcgcgc gccgcgctcg
     2941 aatccatcgc ctaccaaagc gccgcgctgc tgtcggccat gagccgcgac gccgtggcca
     3001 acggcggcgc cccggtgagc gaactgcgcg tggacggcgg tgcctgcgtc aacgacctgc
     3061 tgatgcagtt ccaggccgac ctgctgggca ttcccgtggt gcgcccggcc gtggtcgaaa
     3121 ccaccgccct gggcgcggcc tacctggcgg gcctttccag cggcgtgtat gccagccagg
     3181 acgagctgac cacgctgtgg caggccgagc gccgcttcat gcccacgctg gcgcgcgagc
     3241 aggcccaggc gctgatggcg cgctgggagc atgccgtggc ccaggccgtg ctgccagcct
     3301 gagggcgaga taaaaaacat agctgctccc gcttggtgga ccttgatttc aggcaaaaac
     3361 agcctgtaac tgaatactgg caagcgcaaa cagctattgt ttttgggaca ggacaccagt
     3421 gcacaatggc gccccacgcc ctttctgcac gcccattcca tgagccacag ccaccagccc
     3481 cttcccccca tcgccttcat cggcggcggc aacatggcca gcgccatcat cggcggcctg
     3541 atccagcaag gcctgcccgc cagccagatc gaggtcgtcg agcccttcga gccggcgcgc
     3601 gccgcgctgc tgcaaggttt cggcgtcagc gcccagccca cggccacggc ggcgctggcg
     3661 cgtgctgccc tggtggtttg ggccgtcaag ccgcagacct tcaaggatgc cgcgcaacag
     3721 gcggcgccgc acacccaggg cgcgctgcac ctgagcgtgg ccgccggcat tcgttccggc
     3781 agcattgcgc aatggctctc cagcgagcgc atcgtgcgcg ccatgcccaa caccccggcg
     3841 ctgatcggcc agggcatgac cggcctgtat gcacgcgccg ccgtcactgc ggatgagcgc
     3901 acgctggtcg agcaggtggt ggccaccacc ggcgcctggc tgtggctgga gcaggagtcg
     3961 ctgctggacg ccgtcaccgc cctgtcggga tcgggcccgg cctacgtgtt ctactttctg
     4021 gaggccatga ccgaggccgg cgaagacatg ggcctgacgc gcgagcaggc gcaccagctg
     4081 gccgtggcca cgttcgccgg cgcctccgag ctggcgcgcc gctcggacga accgccggcc
     4141 gtgctgcgcc agcgcgtgac cagcaagggc ggcaccacgc atgcggccat agagtccatg
     4201 gaagccgacg acatggccca gcgcttcatg cgtgcgctcg acgcggccga gcaccgggcg
     4261 cgcgagctgg gcgacgagtt cggcgccacc aactgaaaca catagccatc gccgcgccac
     4321 gccgcccgca ggtacagccc ataccccatc tcacccgtcc ctggttccgc ctgtccctgc
     4381 tgggcgcctg cctggccctg tcgtcctgcg gcggtggcgg ctatgaaact ccgccgacgg
     4441 cgcccgcacc gcagccccag ccacaaccgc cgcaaagcca ggccccattc acccccggcc
     4501 ccggcgacct gccgcaggcc cagggcctct acccgtatgc ggcgccccag ctgctgacgc
     4561 tggacctgcg ctaccagagc acccccgttt ccggcgctgg cctgtatttg atggacaaga
     4621 acacctgcgc gcagctagcc agcccgccac cgagctaccc cggcggcatg gtcacgctgg
     4681 acacggtaaa cctggacgag aacaaggccg acgactgcga gcccgagatc aaggtgctgg
     4741 ccacaagcgg cgacggcctg ctcaagggcg ccgccgccaa gatgcgcctg cccggcagca
     4801 gcacgcgcct ggccacgctc aagtcctacc gcgtcaagtt cgacaagggt gcggactggc
     4861 agggcgagga cacgctgaac ctgaacaagc acccctacga cctgacccgc gtgcgcaaca
     4921 agctggcctt cgacctgatg cgccaggtgc cctaccacca aagcctgcgc acgcagttcg
     4981 tcgagatccg ctacgacgcg ggcgcgggca cgggtgccgg cgcgcaaccc atgggcctgt
     5041 tcacccatat cgaaaagctg ggcggcaaga gctacctgga gcgccgcggc tgggtcgccg
     5101 gctccaacgt ctacaaggtc gccgcgttca acttcaaccc ggacgcgcga ttgcagacgc
     5161 tggccgacgg caaggccggg ccgggctttg aagaactgct ggagatcgag aacgattccg
     5221 gcaaccatgg cgccatcgtg cagacggtga aggcgctggc cgacgatggc acgccgtttg
     5281 cgaccagctt caaccgccac ttcgaccgca acaactacct ggcctggctg gccagcgcca
     5341 tcctgctggg caactgggac acggtgaacc agaacttcgg cctgtaccag ccgctgggcg
     5401 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggccttt gccgacggca
     5521 ccctggtgct gcaacccgcg ccggtctcgg gccgcacgct gctgaacctg ggggcgctgc
     5581 ccgccgggca atacctggtg cgtgtgaccg ccagcgacgc cgacggcaac cgcagcaacg
     5641 cctttgacag caccagcttc gacggccaga ccgtggacgg cgccatctgc ctgaacatgc
     5701 ccggcgccac gccctgctga acgccttcag cgcagcgcga aacccagggc cacgccggca
     5761 aagatggccg cgccaatcca gtggctcttg ctgaaggcga caaagcagcc cgcgcgcgtg
     5821 cgcgtgcgga tcagccggta atgccacagc acctgggccg ccgccaggcc caggcccagc
     5881 cacagcggcc agcccagccg caagggcacc agcaccgccg ccgtcagtgc caggcaggcc
     5941 acgaagaaca gcatgatggc ggccacatca aagcgcccca gcgtgatggc cgaggtcttc
     6001 atgccgatca gcaggtcgtc gtcacggtcc accatggcgt actcggtgtc gtaggccagc
     6061 accaggaaca tgttggctag ccacagcacc caggcctggc ccggcacgcc gcccgtcacc
     6121 gcggcaaagg cgatcacgat gccaaagttg aaggcaatgc ccagaaacgc ctgcggcatg
     6181 gcaaagaagc gcttggtgaa cggatacagg atggtgaaga ccaccgccgg caccgaccag
     6241 gccacggcct gccagcgcgt ggtcagcacc agggccaggg ccaccagcgc cagcgccgcg
     6301 cccagcaggg ccgcctcggt cacgctgacc aggccgctgg tgatggggcg cgcctgcgtg
     6361 cgcttcacat ggcggtcaaa gccgcggtcg gcaatgtcgt tgatgcagca gccggcgctg
     6421 cgcatgagca ccgttccggc gacgaacacg gtcagcagat gccagcccgg aaagcccccc
     6481 gccgccaccc acagcgccac cagcgtgggc cagaccagca ccagccagcc ggccgggcgg
     6541 ttgaagcgga tcagatccag gtagagcgag agcctggagc gcggcggcgt ggcggtcatg
     6601 aacggagtct tcaaataaaa aagggccgaa gcccttgtgt acaaagcgta aaacgctatt
     6661 aatcttctag caaagagcgc agcatccagg ccgtctgctc atgcaccgtc atgcgctggg
     6721 tgagcaggtc ggccgtgggc tcgtcgcagg ccttgtcgga cagggggaag agctcgcgcg
     6781 cggtgcgtgc cacggcctcg tggcccagca ccaggatgcg caccatctcc agcgccttgg
     6841 gcggatcggc gggtacgtcg tccagcgacg acagcttgcc aaacgccgcg tacgagcccg
     6901 gcgccacatg gcccagcgcg cgtatgcgct cggcgatcgg gtcaacggca ttccacagct
     6961 cggtgtactg ctccatgaac atggtgtgca gcgtgttgaa catggtgccg gtcacgttcc
     7021 agtggaagtt gtgcgtggtc aggtacagcg tgtaggtgtc ggccagcagg cgcgacaggc
     7081 cctgggcaat ggcggctctg tccttgtcgc tgatgccgat gcggatgccc ggggcggcaa
     7141 tgccctgggc ggaagcgggc gtcttcacgg tcttgctggt ttttgcggct ttggccatgg
     7201 tgctttctcc ttcggttgac ttgataaaga ctgtagcgca gcagggctgc ctcgccatga
     7261 cttgtggcaa ttgcgggcat agccaacgcg gcccgcttca gctcaggcgc ttgacccccg
     7321 gcagctcgca ggcgtagatg gcgttgcgca gcgcggcgat ggcctcgtag cgcgtgaagc
     7381 tgcggcgcca ggccagcacc acgcggcgca tgggcggggc gctgccgtcg ctctccttga
     7441 tgggcaggta gcggatatgc gcgtcgtcgc tcttgcgccg gcgcacgccg gtgtgcagcg
     7501 cgtcgcgcgg caccgacagg cgcggcacca gggtcacgcc catgccggcc gagaccatgt
     7561 gcttgatggt ctccagcgag ctgccctcga aggtgcggcg tatgccctcg gcattgctgg
     7621 caaagcgcgc gaactcgggg cagacctcca gcacatggtc gcgaaagcag tggcccgcgc
     7681 ccagcagcaa catggtctcg ttcttcagct ccagggccga cacgttcttg cgctcggcca
     7741 gcgggtggct ggatggcacg gccgccagaa agggctcgtc gtacagcggc gcgatggcca
     7801 ggcccgtgtc gggaaagggc tcggccagga tggcgcagtc gatctcgccg gtgcgcagca
     7861 tctccaatag cttgacggtg aagttctcct gcagcatcag cggcatctgc ggcgtgagcg
     7921 cgatcgcgtg gcgcaccagg tcgggcagca gataggggcc tatggtgtaa atcacgccca
     7981 gggtcagcgc gccggccagc gggtccttgc cgcgcttggc gatctccttg atctcggc
//
LOCUS       DAOMTQ010000134         7972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2026_length_7972_cov_24.371479, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000134 DAOMTQ010000000
VERSION     DAOMTQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_7972_cov_24.371479"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK518_12030"
     CDS             <1..546
                     /locus_tag="PK518_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM49688.1"
                     /translation="RDVFYYVTLMNENYAQPDLPETAHPGVLRGCYLFDSYPRLSDGR
                     EAPVSSAKQVTLLGSGAILTEVVKAAELLAAEGIGAQVLSVTSWSELARDGQACEDSG
                     ATPWIAQVLASCGGPIVAATDYVRAVPESVRAYVPVGRAYRTLGTDGFGRSDTRAALR
                     SFFRVDAAHIAAAARQALAQG"
     gene            711..>1842
                     /locus_tag="PK518_12035"
     CDS             711..>1842
                     /locus_tag="PK518_12035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRM49689.1"
                     /translation="MSHIQRSALIDCTKGLACAAIVWHHLAFYGPMSDQLGQVLPGLM
                     AWLQEDARMAVQVFLVLGGFLAAASLAPRGHARFDDPWQRMARRFVRLVLPYAVALVV
                     AILVQAAVRPWFAHDSVSAEPGLWQLLAHALLLQGVLGEESLSAGVWYVAIDFQLFVL
                     TTLLLAGATTAATRLRARWGGDRLARRWPWAMTGAQLLVVLGVAASLLGFNRAAQWDN
                     WALYFFGAYGLGMLAYWAVQSRRPWGAWGWALLLAALVLAALVLEWRARIALAGATAL
                     LLVPALRLPAVARWGGWAPLNRLGQISYSVFLVHFAVCVAVNAVVSWFWPQALGAVAA
                     GMVLAFVLSIAAGHLLYERVERQLPSWQEALRWQAGMVGAGLI"
     assembly_gap    1843..1942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1943..2649)
                     /locus_tag="PK518_12040"
     CDS             complement(<1943..2649)
                     /locus_tag="PK518_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRM49690.1"
                     /translation="MNAQEIWQFISTQGAEFGIKLLTAIAAWVVGRWLISLAVRLMGK
                     VFERGNKIDATLSQYLQSIASVLMNIVLILAILDIFGVKTTSFAALLAGAGLAIGAAW
                     SGMLGNFAAGVFMQVLRPYKVGDFISAGGVVGTVKELGLFATTVITPDNVVTVVGNGK
                     VFGDNIQNYSALPVRRVDCVAKVANSVNPLDAIARLQPVIAAIPNVATDPAPVIEILE
                     FTPEGPKLCVRPFTHTD"
     gene            complement(2731..3591)
                     /gene="fghA"
                     /locus_tag="PK518_12045"
     CDS             complement(2731..3591)
                     /gene="fghA"
                     /locus_tag="PK518_12045"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HRM49691.1"
                     /translation="MSDTLELLSSHACFGGEQRFYRHASREIGLPMQFSVYLPPQALA
                     GGKCPALLYLAGLTCTEETFMVKAGAQQQAAELGLALIAPDTSPRGAGLPGEADAWDF
                     GVGAGFYLDATQAPWAAHWRMESYLLDELLPLAGSALPIDGARLGIFGHSMGGHGALT
                     LALRHPGRFKSLSAFAPICAPTRCPWGVKAFTGYLGPDRAGWGAHDATGLMEQQSTAP
                     YPAGILIDQGLADKFLAEQLHPELFNAACALVGQPLTLRRHEGYDHGYYFIQTFVQDH
                     LRHHAQQLAG"
     gene            complement(3699..4817)
                     /locus_tag="PK518_12050"
     CDS             complement(3699..4817)
                     /locus_tag="PK518_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRM49692.1"
                     /translation="MKSRAAVAFKAGEPLQIIEIDVAPPQPGEVLIRITHTGVCHTDA
                     FTLSGDDPEGIFPVVLGHEGAGIVVECGAGVTSVKPGDHVIPLYTAECGQCLFCKSGK
                     TNLCTAVRATQGKGVMPDGTTRFSYDGKPLYHYMGCSTFSEYTVVAEVSLAKVSPEAN
                     HEQVCLLGCGVTTGLGAVKNTAKVQPGDTVAVFGLGGIGLAVIQGAKLAKAGRIIAID
                     TNPSKFDLARTFGATDCVNPKDHEQPIQQVIVEMTGWGVDHSFECIGNVNVMRAALEC
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRRWLGTAFGGVKGRSELPGMVEDAMAGK
                     IQLAPFVTHTMGLADINRAFELMHAGKSIRSVINFATL"
     gene            complement(5004..6515)
                     /locus_tag="PK518_12055"
     CDS             complement(5004..6515)
                     /locus_tag="PK518_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRM49693.1"
                     /translation="MELIEHLSLGLQVAFTAQNLLYAFLGCLLGTLIGVLPGLGPVAT
                     IAMLLPTTYALPPTAALIMLAGIYYGAQYGGSTTAILVNLPGESSSVVTVIDGYQMAR
                     KGRAGPALASAALGSFFAGCVGTLVIAAFAPPLTEVAFKFGPAEYFSLMVLGLIGAVV
                     MASGSLLKAITMILVGLAFGLVGTDVNSGVARYSFDIPELTDGISFVAIAMGIFGYGE
                     IIANLSVPTHQREVFTDKVTGLMPTKEDFKRMFPAVLRGTAIGSALGILPGGGALLSA
                     FAAYTIEKKSRLKPGEIPFGQGNIRGVAGPESANNAGSQTSFIPLLTLGIPPNAVMAL
                     MVGAMTIHNIQPGPQVMTSNPELFWGLIASMWIGNLMLVVLNLPMIGLWIKLLSIPYK
                     WLFPAIVLFCAIGVYSENNNTFEIWMVAIFGVLGYVFHKLACEPAPLLLGLILGPMME
                     ENLRRALLLSRGEWSVLVTRPLSASLLAVAAVLVIIVMLPAVSKKREEAFVED"
     gene            complement(6547..7035)
                     /locus_tag="PK518_12060"
     CDS             complement(6547..7035)
                     /locus_tag="PK518_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRM49694.1"
                     /translation="MKINSQRDFTAGLLFSAFGVAFAWGATTYNVGSGARMGPGYFPL
                     IVGILIAVMGVLITARSFITHSEDGDPIGAIAWRPLGFIIGANLVFGVLLGGLPSIGL
                     PPMGLVVAIYALTLIASLAGDQFQLKGVLVLATVLSVGSYLAFVVALNLQFPVWPTFI
                     TG"
     gene            complement(7235..>7972)
                     /locus_tag="PK518_12065"
     CDS             complement(7235..>7972)
                     /locus_tag="PK518_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM49695.1"
                     /translation="FARLEQDFNHMLDRIEQLMAGVTHVSDAIAHNLRTPLTRLRLRL
                     QHAHDGAADAAALRAALGSAMHDIDDLSRVLDKLLSIAQAESGTRRAPFTPQSWGEIG
                     ADVVELYEDLAEHEGVTLTWECTHPAPLLGDRSLLAGALVNVVDNAIKYGGRGASVRV
                     QSRSARDADGAACSEIWVQDDGPGAPPEALATLGERFLRLRPDLPGHGLGLASVRAVM
                     QLHGGSMRLEPARPGLRVALRLPQHTP"
BASE COUNT         1251 a         2537 c         2751 g         1333 t
ORIGIN      
        1 cgcgacgttt tctactacgt cacgctgatg aacgagaact acgcccagcc cgacctgccc
       61 gaaaccgcgc acccgggggt gctgcgtggt tgctacttgt tcgatagcta tccgcgcttg
      121 tcggacgggc gcgaggcgcc tgtttcatct gcaaaacagg tgacgctgct gggctcgggc
      181 gccatcctca ccgaggtcgt caaggccgcc gagctgctgg cggccgaggg cattggcgcg
      241 caagtgctca gcgtgaccag ctggagcgag ctggcgcgcg atggccaggc ctgcgaggac
      301 agcggtgcga cgccctggat cgcccaggtg ctggcgagtt gcggcggccc catcgtcgcc
      361 gccaccgact atgtgcgcgc cgtgcccgag agcgtgcgtg cctatgtccc ggtcgggcgt
      421 gcctaccgca cactgggcac cgacggcttt ggccgcagcg acacgcgcgc cgcgctgcgc
      481 agcttcttcc gggtggatgc ggcgcatatt gccgcggccg cccggcaggc cctggcccag
      541 gggtgaaggg cgggtaaagc gcataaagcg ggctcgggcg cggcggcggc gcgcagtttg
      601 taaccaatgg ggtggctggc gcgttatgcc tggcattgcg cccctttgcc gagcccgatg
      661 ccctgggcgg cgctgaaccg tgcgcacccg ttcatctccc gccatctttc atgtcccaca
      721 ttcaacgcag cgcccttatc gactgcacca agggcctggc ctgcgcggcc atcgtctggc
      781 accacctcgc tttctatggc cccatgtccg accagctggg ccaggttctg cccggcctca
      841 tggcctggct gcaggaagat gcgcgcatgg cggtgcaggt gtttctggtg ctgggtggct
      901 ttctggcggc tgccagcctg gcgccgcgtg gccatgcgcg gtttgatgac ccctggcagc
      961 gcatggcgcg gcgctttgtg cgcctggtgc tgccctatgc cgtggcgctg gtcgtggcca
     1021 ttctggtgca ggcggcggtg cggccgtggt ttgcgcatga ctcggtctct gccgagcccg
     1081 gcctgtggca gctgctggcg catgcgctgc tgctgcaggg cgtgctgggc gaggagtcgc
     1141 tgtcggccgg ggtctggtat gtggccatcg actttcagct gttcgtgctg accaccttgc
     1201 tgctggccgg tgcgaccacg gccgccacgc ggctgcgcgc gcgctggggt ggcgaccggc
     1261 tggcgcgccg ttggccctgg gccatgaccg gcgcgcagct gctggtggtg ttgggggtgg
     1321 ccgcgtcgct gctgggcttc aaccgcgctg cgcagtggga caactgggcg ctgtactttt
     1381 ttggcgccta tgggctgggc atgctggcct attgggccgt gcagtcgcgg cggccctggg
     1441 gtgcctgggg ctgggcgctg ttgctggccg ccctggtgct ggcggcgctg gtgctggagt
     1501 ggcgcgcgcg catcgcattg gccggcgcca cggcgctgct gctggtgccg gccctgcgcc
     1561 tgccggccgt ggcgcgctgg ggcggctggg cgcccttgaa ccggctgggg cagatctcct
     1621 actcggtctt tctcgtccac tttgccgtgt gcgtggccgt gaacgccgtg gtcagctggt
     1681 tctggccgca ggccctgggg gcggtagccg cgggcatggt actggcgttt gtgctgtcga
     1741 ttgccgccgg ccatctgctg tacgagcgcg tcgagcgcca actgccttcc tggcaggagg
     1801 cgctgcgctg gcaggcgggc atggtgggtg ccgggctgat cannnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nntggtcggt gtgggtgaag gggcgcacgc acagcttggg
     1981 gccctcgggc gtgaattcca ggatctcgat caccggcgcc gggtcggtcg ctacgttggg
     2041 gatggcggcg atcaccggtt gcaggcgcgc aatcgcgtcc agcgggttga cgctgttggc
     2101 caccttggcc acgcaatcga cgcggcgcac gggcagggca ctgtagttct ggatgttgtc
     2161 gccaaacacc ttgccgttgc ccaccacggt aaccacgttg tcgggcgtga tgacagtggt
     2221 ggcaaacagg cccagctctt tcacggtgcc caccacgccc ccggcgctga tgaagtcgcc
     2281 caccttgtac gggcgcagca cctgcatgaa cacgccggcg gcaaagttgc ccagcatgcc
     2341 gctccaggcc gcgcctatgg ccaggccggc gccggccagc agggcggcaa acgaggtggt
     2401 cttgacgccg aagatgtcca ggatggccag gatcaggacg atgttcatca gcaccgaggc
     2461 gatggactgc aggtattgcg acagcgtggc gtcgatcttg ttaccgcgct cgaatacctt
     2521 gcccatcagg cgcaccgcca ggctgatgag ccagcggccc accacccagg cggcaatcgc
     2581 cgtgagcagc ttgatgccga attcagcccc ttgggtactg atgaattgcc agatttcttg
     2641 ggcgttcatg gagcctcctg ctgcaaaaaa agggctccat cctatggcct gtgactatcg
     2701 accaatgcaa atcattgcaa acgcctgatg ctatccggcc agttgctgcg cgtggtggcg
     2761 caggtgatcc tggacaaagg tttgtatgaa gtagtagcca tggtcgtagc cctcgtggcg
     2821 gcgcagcgtg agcggctggc cgacgagcgc gcaggcggcg ttgaacagct ccgggtgcag
     2881 ctgctcggcc aggaatttgt cggccaggcc ctggtcgatg aggatgccgg ccggataggg
     2941 cgctgtgctc tgctgctcca tgagcccggt ggcgtcatgc gcgccccagc cggcgcgatc
     3001 cgggcccagg tagcccgtga aggccttcac gccccagggg cagcgcgtgg gcgcgcagat
     3061 gggtgcgaag gccgacaggc tcttgaagcg ccccgggtgg cgcagcgcca gcgtgagcgc
     3121 gccatgccca cccatgctat ggccaaaaat gcccagccgc gcgccgtcga tgggcagggc
     3181 gctgccggcc agcggcagca gctcgtccag cagatagctc tccatgcgcc agtgcgcggc
     3241 ccatggcgcc tgcgtggcat cgaggtagaa gcccgcgccc acgccaaagt cccaggcgtc
     3301 cgcctcgccg ggtaggccgg cgccgcgtgg actggtgtcg ggggcgatca gggccaggcc
     3361 cagctctgcc gcctgctgct gcgcgccggc cttgaccatg aaggtctcct cggtgcaggt
     3421 caggccggcc aggtagagca gggcgggaca tttgccaccg gccagcgcct ggggcggcag
     3481 gtagacggag aactgcatcg gcaggccgat ctcacgcgag gcgtggcggt agaagcgctg
     3541 ctcgccgcca aagcaggcgt gactggagag caattccagg gtgtcggaca tgggggtgta
     3601 agctcctata ttggtagctg ttcacgctta gtttgaagca aattcacata aaaaatgccg
     3661 tgaacccatt gaaatgaaag cgcaaacagc tctctttttt acagcgtggc gaagttgatc
     3721 accgagcgta tggacttgcc cgcgtgcatc agctcgaagg cgcggttgat gtcggcaagc
     3781 cccatggtgt gcgtgacaaa gggcgccagc tggatcttgc cggccatggc gtcttccacc
     3841 atgccgggca gctcgctcct gcccttgacg ccgccaaacg ccgtgcccag ccacctgcgg
     3901 cccgtgacca gctgaaacgg gcgggtactg atttcctggc cgctgcctgc cacgccgatg
     3961 atgaccgact ggccccagcc gcggtgcgcg cattccagcg ccgcgcgcat gacgttcacg
     4021 ttgcctatgc actcgaagct gtggtccacg ccccagccgg tcatctccac gatcacctgc
     4081 tggatgggct gctcgtggtc tttcggattg acgcagtcgg tggcgccaaa ggtgcgcgcc
     4141 agatcgaact tggaggggtt ggtgtcgatg gcgatgatgc ggccggcctt ggccagtttt
     4201 gcgccctgga tcaccgccag gcctatgccg cccagaccga acacggctac cgtatcgccc
     4261 ggttgcacct tggcggtgtt cttcaccgcg cccaggccgg tggtcacgcc acagcccagc
     4321 aggcacacct gctcgtggtt ggcctcgggg ctgaccttgg ccaacgagac ctcggccacc
     4381 acggtgtatt cgctgaacgt cgagcaaccc atgtagtggt agaggggttt gccgtcgtag
     4441 gaaaagcgtg tcgtgccgtc gggcattacg cccttgccct gggtggcgcg cactgccgtg
     4501 cacaggttgg tcttgccgct cttgcagaac aggcattggc cgcattcggc ggtgtagagc
     4561 gggatcacat ggtcgcccgg cttgacgctg gtcacgcccg caccacattc aacgacgatg
     4621 cccgcgccct cgtggccgag cacgacgggg aagatgcctt cggggtcgtc acccgagagg
     4681 gtgaaggcgt cggtgtggca tacgccggtg tgggtgatgc ggatcagcac ctcgcccggc
     4741 tgcggcgggg cgacgtcgat ctcgatgatc tgcaggggct ctccagcctt gaaggcaacg
     4801 gcggcgcggg atttcatgga aacgctctcc tctggcgaat gattggggga tatggcgggc
     4861 agggcaggcc ccccgcatgg tgcaaggccc gtagtatggc ccgcccgcgc gcggccccgg
     4921 catgacaaag gcgcccggcc ccaggcccgc aatgggcatg ggccgggcgc ccgggcgccg
     4981 gatgtcggga ggctgtaggg tggtcaatcc tccacgaagg cctcctcgcg cttcttgctc
     5041 acggcgggca gcatgacgat gatcaccagc accgcggcca ccgccagcag cgaggccgac
     5101 agcgggcgcg tgaccagcac gctccactcg ccgcgcgaca gcagcagggc gcggcgcagg
     5161 ttctcctcca tcatcggccc caggatcagg cccagcagca gcggcgcggg ctcgcaggcg
     5221 agcttgtgga acacatagcc cagcacgcca aagatggcca ccatccagat ctcgaaggtg
     5281 ttgttgttct ccgagtacac gccgatcgca cagaacagca cgatggccgg gaacagccac
     5341 ttgtagggga tggacagcag cttgatccac agcccgatca tgggcaggtt cagcaccacc
     5401 agcatcaggt tgcctatcca catcgaggcg atcaggcccc agaacagctc cgggttgctg
     5461 gtcatcacct gcgggccggg ctggatgttg tggatggtca tggcgcccac catcagcgcc
     5521 atcacggcgt tgggcggaat gcccagcgtc agcagcggaa tgaaggaggt ctgcgagccc
     5581 gcgttgttgg ccgactcggg gccggccacg ccgcggatgt tgccctggcc gaaaggtatc
     5641 tcaccgggct tgagcctgga cttcttctcg atggtgtagg cggcaaaggc cgacagcagc
     5701 gcgccgcccc cgggcagtat gcccagcgcc gagccaatgg ccgtgccgcg cagcacggcg
     5761 gggaacatgc gtttgaagtc ctccttggtg ggcatgaggc cggtcacctt gtcggtgaac
     5821 acctcgcgct ggtgcgtggg tacggacagg ttggcgatga tctcgccata gccaaagatg
     5881 cccatggcaa tcgccacgaa gctgatgccg tcggtcagct ccgggatgtc gaagctgtag
     5941 cgcgccacgc ccgagttcac gtccgtgccc accaggccaa aggccaggcc gaccaggatc
     6001 atggtgatgg ccttgagcag cgagcccgag gccatgacca ccgcgccgat caggcccagc
     6061 accatcagcg agaaatactc ggccgggccg aacttgaagg ccacctcggt cagcggcggg
     6121 gcaaaggcgg cgatcaccag cgtgcccacg cagccggcga agaacgagcc cagcgcggcc
     6181 gaggccagcg ccggcccggc ccggcccttg cgcgccatct ggtagccgtc gatcaccgtg
     6241 accaccgagg acgactcgcc cggcaggttc accaaaatgg cggtggtcga gccgccgtac
     6301 tgcgcgccgt agtaaatgcc ggccagcatg atcagcgctg ccgtgggcgg cagggcgtag
     6361 gtggtgggca gcagcatggc aatggtggcc accggcccca ggcccggcag cacgccgatc
     6421 aaggtgccca gcaggcagcc caggaaggcg tacaacaggt tctgcgccgt gaacgcgacc
     6481 tgaagcccca gtgaaagatg ttctatcagt tccatgatga ttcctcgtga tgggttgcgc
     6541 ccgggctcag ccggtgatga aggtgggcca taccgggaac tgcaggttca gcgccacgac
     6601 gaaggccagg tagctgccca cggagagcac ggtggccagg acgagcacgc ccttgagctg
     6661 gaactggtcg cccgccaggc tggcgatcag cgtcagcgca tagatggcga ccaccagccc
     6721 catgggcggc aggccaatgc tgggcaggcc gcccagcagc acgccaaaca ccaggttggc
     6781 gccgatgatg aagcccagcg gacgccaggc aatcgcgcct atggggtcgc catcctcgga
     6841 atgcgtgatg aaggaacgcg cggtgatgag cacgcccatc accgcgatca gaatgccgac
     6901 gatgagcggg aagtagcccg gccccatgcg ggcgccgctg cccacgttgt aggtggtggc
     6961 gccccaggca aaggccacgc caaaggcgct gaacagcagc ccggcggtga agtctctctg
     7021 gctattgatt ttcaaggcaa aggtctcctg accggcctgc gtgcaggccg ggttggtgtg
     7081 tatagagggg gagcgggcat cgtaggcgcc atgccgcgca agcggggtga ccaaagcatt
     7141 gccaaacggt aatgcttgcc gctgttaact ataaaaataa tagctattgg cgctttccca
     7201 ttaagcgcta aaggcttatt tttctattga tacttcaggg tgtgtgctgc ggcaggcgca
     7261 gcgccacgcg caggccgggc cgggccggct ccagccgcat gctgccgcca tgcagctgca
     7321 ttaccgcgcg cacgctggcc agtcccaggc catggccggg caggtcgggg cgcaggcgca
     7381 ggaagcgctc gcccagtgtg gccagagcct cgggcggcgc cccggggcca tcgtcctgca
     7441 cccagatttc gctgcaggcg gcgccgtccg cgtcccgcgc gctgcgcgac tgcacgcgca
     7501 cgctggcgcc ccggccgccg tacttgatgg cgttgtccac cacgttcacc agcgcgccgg
     7561 ccagcaggct gcggtcgccc agcagggggg ccgggtgcgt gcattcccat gtcagcgtga
     7621 cgccctcgtg ctcggccagg tcttcgtaca gctcgaccac gtcggcgccg atctcgcccc
     7681 atgactgcgg cgtgaagggc gcgcggcgcg tgcccgattc ggcctgggcg atggacagca
     7741 gcttgtccag cacgcggctc aggtcgtcga tgtcgtgcat ggcgctgccg agtgccgcgc
     7801 gcagcgcagc ggcatcggcc gcgccgtcat gcgcgtgctg caggcgcagg cgcagccggg
     7861 tgagcggcgt gcgcaggttg tgcgcgatgg cgtcggacac atgggtcacg cccgccatga
     7921 gctgctctat gcggtcgagc atgtggttga agtcctgctc cagccgcgca aa
//
LOCUS       DAOMTQ010000135         7768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2123_length_7768_cov_25.392714, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000135 DAOMTQ010000000
VERSION     DAOMTQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2123_length_7768_cov_25.392714"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PK518_12070"
     CDS             complement(<1..638)
                     /locus_tag="PK518_12070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRM49696.1"
                     /translation="MSPAHFVLHDVFGYEQFRGPQQAIIEHVIAGGDALVLMPTGGGK
                     SLCYQVPAIVRRDAGHGVTVVVSPLIALMHDQVGALHEAGVEAAFLNSTLDWQQTLHV
                     ERRLSRGEITLLYAAPERVTTERFIGLLDDLHARGQLSLFAIDEAHCVSQWGHDFRPE
                     YRQLNLLHDRYPGVPRIALTATADGLTRADIVAGLQLSDARQFVSSFDRPNIR"
     gene            854..1816
                     /locus_tag="PK518_12075"
     CDS             854..1816
                     /locus_tag="PK518_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49697.1"
                     /translation="MQTLLRGTLAAALAFSATATLAQAYPDKPVTLVVPFAAGSGTDS
                     VARAVASGLAARLKQPVIVDNKPGASAQVAAQYVAKAKPDGYTLFMTTNTSHSANPAL
                     YKSLKYDPIKDFTPVARVGELPFALAVNPAVPAKTLAELLDYARANPGKLSYATPNST
                     SLVAMESIKHIAKIDVLGVPYKSSPQAMTDLVGGQVQIYVADLGSGMGMLKTDKVRAL
                     GVTTAQPTPMLPGVPPIGQTVKGFDLTSWNGIFGPAGLPQPMVQRLNTELQAVLADKD
                     LQDKLAQIGFQVWPSKTPEEFTQYVAGQLTHWRTLIQQAGIQPE"
     gene            1826..2209
                     /locus_tag="PK518_12080"
     CDS             1826..2209
                     /locus_tag="PK518_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM49698.1"
                     /translation="MTTSRYLVRAAGVPSYQPANHEHTFNQRLIGPETVGARQMEVLL
                     GTLHKGGGALPHAHPGIEQACYLLEGQARASVEGEAFDMLPGDMCFFPADRMHTFTVT
                     SDTPVKLLVIYSPPYGESPDKVIRP"
     gene            2206..3369
                     /locus_tag="PK518_12085"
     CDS             2206..3369
                     /locus_tag="PK518_12085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM49699.1"
                     /translation="MNFAPTPEQEQIKAAIERVCEPFDADYWLKKDQEGGFPHDFHQA
                     LAASGWLGIAMPEEYGGAGLGISEAALMMHTISATGAGLSGASAVHMNIFGLHPVVVF
                     GTEEQKKRWLPPLIEGKDKACFGVTEPNAGLNTLKLQTRAVRDGNHYVVHGQKVYIST
                     AQVANKILLLTRTTPVEQARGTEGLTLFYTDLDRSKIEVHEIAKLGRGCVDTNQLFID
                     GLRIPVEDRVGEEGKGFSYILHGMNPERILIAAEAVGLGRAALARAAGYAAEREVFGR
                     PIGKNQAIQHPLAEAWMDLEAAHLMVQKAAWLYDQGKNCAAEANAAKFLGAEAGYRAC
                     ERAIFTHGGMGYAKEYHVERYMRESWIPRLAPVSPQLILCFIAEKVLGLPKSY"
     gene            3395..4285
                     /locus_tag="PK518_12090"
     CDS             3395..4285
                     /locus_tag="PK518_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49700.1"
                     /translation="MKLHQLRYFAVLAEELHFGRAAERLAITQPPLSSGIKALEEELG
                     VRLFERSSKHVALTAAGQAYLAEVRVVLDRVASAAETARAVAAGLQGRLEIGFAGSMV
                     YRDMPRIVRAFGARAPGIDVNLRELSSAEQLDALLHRQLHAGFINAAAVPPRLACRPL
                     IPDHFVCCLPEAHPLARESAVALPALAQEGFVMFARDVAPANHDNVIALFERAGIHPR
                     TRHAARQWLTVVSLVALGMGVALVPASLAQAGVQGVRFLPIAGLHHPAVGVLAWHEEA
                     DHPALRVFLDCAAPFLQSGA"
     gene            4282..5316
                     /locus_tag="PK518_12095"
     CDS             4282..5316
                     /locus_tag="PK518_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRM49701.1"
                     /translation="MTLPHYTRGKELPAILEQRIAILDGAMGTMIQRFKLTEAQYRGA
                     RFANFARDVKGNNELLSLTRPDVIRDIHEGYLAAGADLIETNTFGATSIAQDDYGMGD
                     LAYEMNLESARLARAACDKFSTPDKPRFVCGALGPTPKTASISPDVNDPGARNISFEQ
                     LRAAYLEQTLALIAGGADVILVETIFDTLNAKAALFAVDEAFEQTGECLPIMISGTVT
                     DASGRILSGQTVTAFWHSVRHANPLSIGLNCALGAALMRPYIQELAKAAPDTFISCYP
                     NAGLPNPMSDTGFDETPDVTSRLVHEFAAEGLVNILGGCCGTTPDHIAAIARAVAPVG
                     TRRLFTAEPA"
     gene            5772..5966
                     /locus_tag="PK518_12100"
     CDS             5772..5966
                     /locus_tag="PK518_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49702.1"
                     /translation="MPAAPTTGADGYSAERLALGQACHSQPRAQLVAKGPGFETYHVD
                     CSNGDALSIRCEFGNCRVLR"
     gene            6079..>7768
                     /gene="mgtA"
                     /locus_tag="PK518_12105"
     CDS             6079..>7768
                     /gene="mgtA"
                     /locus_tag="PK518_12105"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HRM49703.1"
                     /translation="MRSMLFRDIFAGFVRTRRILRHYRRLALLETLTRTGVSREPPES
                     LTQALNAAAHSDGAALLTQLGSSAAGLTKTQADAVRERVGPNEVEHEKPLPWWLHLWH
                     SFRNPFNLLLTLLAGISFLTHDTKGALLISTMVVLSTLLRFWQERKSNQAADALKAMV
                     SNTATVLRPAQAAGAEARRMEVAIKLLVPGDLVWLSAGDMIPADCRVLTAKDLFVSQA
                     AMTGESMPVEKSAGLGPAPAGNALDLDNLVFMGTNVVSGSAAAVVLYTGNNSYFGALA
                     SRVIATNRAPTAFYAGVNKVSWLLIRFMFVMAPLVFFINGFTKGDWVEALLFALAVAV
                     GLTPEMLPMIVTSTLAKGAVFLSRKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKIF
                     LARHVDAWGEESGEVLELAYLNSYYQTGLKNLLDVAVLEHAEVSREIEPAKNFHKVDE
                     IPFDFTRRRMSVVVAEHDASHLLITKGAVEEMLTVCTAVRHGETQEPLTPELLTRIRA
                     VTADLNKEGLRVVAVAAKQGPPTQQVYGLADECGLTLIGYVAFLDPPKDSTAAAL"
BASE COUNT         1287 a         2733 c         2579 g         1169 t
ORIGIN      
        1 cggatgttgg gccggtcgaa gctgctgacg aactggcgcg cgtcggaaag ctgcaggccg
       61 gcaacgatgt cggcgcgcgt caggccgtcg gccgtggcgg tgagggcgat gcgcggcacg
      121 ccggggtaac ggtcgtgcag caggttcagc tggcggtact cgggccggaa gtcgtggccc
      181 cactggctca cgcaatgcgc ctcgtcaatg gcgaacaggc tcagttgccc gcgcgcgtgc
      241 aggtcgtcca gcaggccgat gaaacgctcc gtggtcaccc gctcgggcgc ggcgtaaagc
      301 agcgtgatct cgccgcgcga gaggcgccgc tcgacgtgaa gggtctgctg ccagtccagc
      361 gtggagttga gaaaggccgc ctcgaccccg gcctcgtgca gcgcgcccac ctggtcgtgc
      421 atgagggcga tcaggggcga aaccaccacc gtcacgccat ggcccgcatc gcgccgcacg
      481 atggccggca cctggtagca caggctcttg ccgccgcccg tgggcatgag cacgagtgcg
      541 tcgccgcccg cgatcacatg ttcgatgatg gcctgctgcg ggccacggaa ctgctcgtag
      601 ccgaagacgt catgcaggac gaaatgcgcg ggggacaaac cggaaaactc tctttttaat
      661 agctatcagc gcttgatgga taagcgcaaa gtgctgattt gactcaaatt ccgcaacaac
      721 gggcaacgga cgcctattgt gcgcccggtc aatcatttgc aatgcctatt gatcagtccg
      781 aagattgaat tggacatgaa tgattgatcg gcgcaagatg ccgcgcacaa ccatacccat
      841 ataggagaca tgtatgcaga ctctgctgcg cggcaccctc gccgctgccc tggccttcag
      901 cgccacggcg accctggccc aggcctatcc cgacaagccc gtgaccctgg tcgtgccctt
      961 tgcagccggc agcggcacgg actcggtggc gcgtgccgtg gcctcgggcc tggcggcgcg
     1021 gctcaagcag ccggtcatcg tggacaacaa gcccggcgcc agcgcccagg tggcagcgca
     1081 gtatgtggcc aaggccaagc ccgacggcta cacgctgttc atgaccacca acacctcgca
     1141 ttcggccaac ccggcgctgt acaagagcct gaagtacgac cctatcaagg acttcacgcc
     1201 cgtggcgcgc gtgggcgagc tgcccttcgc gctggccgtg aaccccgccg tccccgccaa
     1261 gaccctggcc gaactgctgg actacgcgcg cgccaacccg ggcaagctga gctacgccac
     1321 gcccaacagc acctcgctgg tggccatgga gagcatcaag cacatcgcca agatcgacgt
     1381 gctgggcgtg ccttacaagt ccagccccca ggccatgacc gacctggtgg gcggccaggt
     1441 gcagatctac gtggccgacc tgggctccgg catgggcatg ctcaagaccg acaaggtgcg
     1501 cgcgctgggc gtgaccacgg cccagcccac gcccatgctg ccgggcgtgc cgcccatagg
     1561 gcagacggtg aagggctttg acctgacatc ctggaacggc atcttcggcc ccgccggcct
     1621 gccgcagccc atggtgcagc ggctgaacac cgagctgcaa gccgtgctgg ccgacaagga
     1681 cttgcaggac aagctggcgc aaatcggctt tcaggtctgg cccagcaaga cgccggagga
     1741 attcacgcag tacgtcgccg gccagctgac ccactggcgc accctgatcc agcaggccgg
     1801 catacaaccc gagtgaggcc accccatgac cacatcccgc tacctggtgc gcgccgccgg
     1861 cgtgcccagc taccagcccg ccaaccacga gcacaccttc aaccagcgcc tgatcggccc
     1921 cgagaccgtc ggcgcgcggc agatggaggt gctactgggc acgctgcaca agggcggcgg
     1981 cgccctgccc cacgcccacc cgggcataga gcaggcctgc tacctgctgg agggccaggc
     2041 ccgcgcctcg gtcgagggcg aggccttcga catgctgccg ggcgacatgt gcttctttcc
     2101 ggccgaccgc atgcacacct ttaccgtcac cagcgatacg cccgtcaagc tgctggtgat
     2161 ctacagcccg ccctatggcg aatcgcccga caaggtaatc cgaccatgaa cttcgcaccc
     2221 acccccgagc aggaacagat caaggccgcc atagagcgcg tctgcgagcc cttcgacgcc
     2281 gactactggc tgaagaagga ccaggagggc ggctttccgc acgacttcca ccaggcgctg
     2341 gcggcatcgg gctggctggg catcgccatg cccgaggaat atggcggcgc cggcctgggc
     2401 atcagcgagg ccgcgctgat gatgcacacc atctccgcca ccggcgcggg cctgtcgggc
     2461 gcctcggccg tgcacatgaa catcttcggc ctgcacccgg tggtggtctt cggcaccgag
     2521 gagcagaaaa aacgctggct accgccgctg atcgagggca aggacaaggc ctgctttggc
     2581 gtgaccgagc ccaacgccgg cctgaacacg ctgaagctgc agacacgcgc cgtgcgcgat
     2641 ggcaaccatt acgtggtaca tgggcagaag gtctacatct ccaccgccca ggtggccaac
     2701 aagatcctgc tgctgacacg caccacgccg gtggagcagg cgcgcggcac cgagggcctg
     2761 acgctgttct acaccgacct ggatcgcagc aagatcgagg tgcacgaaat cgccaagctg
     2821 ggccgcggct gcgtggacac gaaccagctc ttcatcgacg gcctgcgcat ccccgtggag
     2881 gaccgggtgg gcgaggaggg caagggcttt tcctacatcc tgcacggcat gaaccccgag
     2941 cgcatcctga tcgccgccga ggccgtcggc ctgggccgcg cggccctggc gcgcgcggcc
     3001 ggctacgccg ccgagcgcga ggtcttcggc cgccccatcg gcaagaacca ggccatacag
     3061 cacccgctgg ccgaggcctg gatggacctg gaggccgcgc acctgatggt gcaaaaggcc
     3121 gcctggctgt acgaccaggg caagaattgc gccgccgagg ccaacgccgc caaattcctg
     3181 ggcgccgagg ccggctaccg cgcctgcgag cgcgccatct tcacccatgg cggcatgggc
     3241 tacgccaagg aataccacgt cgagcgctac atgcgcgagt cctggatccc gcgcctggcg
     3301 ccggtgagcc cgcagctcat cctgtgcttc atcgccgaga aagtgctggg gctgcccaag
     3361 tcgtactgac gcgcgcctgg ggcatgatgg ctccatgaaa ctgcaccagc tgcgctattt
     3421 cgccgtgctg gccgaggaac tgcacttcgg ccgcgccgcc gagcggctgg ccattacgca
     3481 gccaccgctg tcctcgggca tcaaggcgct ggaggaagaa ttgggcgtgc gcctgttcga
     3541 gcgcagcagc aagcatgtgg cgctcacggc cgccggccag gcgtatctgg ccgaggtgcg
     3601 cgtggtgctg gaccgcgtgg ccagcgccgc cgagacagcg cgcgcggtgg ccgcagggct
     3661 gcaggggcgg ctggagatcg gctttgccgg ctccatggtg taccgcgaca tgccgcgcat
     3721 cgtgcgcgcc ttcggcgccc gtgcgccggg catagacgtg aacctgcgcg agctgtcgtc
     3781 ggccgagcag ctggacgccc tgctgcaccg ccagctgcac gccggcttca tcaacgccgc
     3841 cgccgtgccc ccgcgcctgg catgccggcc gctcatcccc gaccatttcg tctgctgcct
     3901 gcccgaggcg caccccctgg cgcgtgaatc agccgtggcc ctgccggcgc tggcgcagga
     3961 gggcttcgtg atgttcgcgc gcgacgtggc gccggccaac cacgacaacg tgatcgcgct
     4021 gttcgagcgc gccggcatcc acccacgcac gcgccatgcg gcgcggcaat ggctcacggt
     4081 ggtgtcgctg gtggcgctgg gcatgggtgt ggccctggtg ccggcgtcgc tggcccaggc
     4141 gggcgtgcag ggcgtgcgct tcctgcccat cgccggcctg caccatccgg cggtgggcgt
     4201 gctggcctgg catgaggagg ccgaccatcc cgcgctgcgg gtgtttctcg attgcgccgc
     4261 gcccttccta caatcgggcg catgaccctg ccccactaca cccgcggcaa agagctgccc
     4321 gccatcctcg aacaacgcat cgccatcctg gacggcgcca tgggcaccat gatccagcgc
     4381 ttcaagctga ccgaggcgca gtaccgcggc gcgcgctttg ccaactttgc gcgcgacgtg
     4441 aagggcaaca acgagctgct gtcgctcacc cgcccggatg tgatccgcga catccacgaa
     4501 gggtatctcg ccgccggcgc cgacctgatc gaaaccaaca cctttggcgc aacatccatc
     4561 gcccaggacg actacggcat gggcgatctg gcctatgaga tgaacctgga gagcgcccgc
     4621 ctggcgcgcg ccgcctgtga caaattcagc acgcccgata aaccacgctt tgtctgcggc
     4681 gccctgggcc ccacgcccaa gacggccagc atcagccccg acgtgaacga ccccggcgcg
     4741 cgcaacatca gcttcgagca gctgcgcgcg gcctacctgg agcagacgct ggcgctgatc
     4801 gccggtggcg ccgacgtgat cctggtcgag acgatcttcg acaccctgaa cgccaaggcg
     4861 gcgctgtttg ccgtggacga ggccttcgag cagacgggtg aatgcctgcc catcatgatc
     4921 agcggcaccg tcaccgacgc ctcgggccgc atcctgtcgg gccagacggt gacggccttc
     4981 tggcacagcg tgcgccacgc caatccgctg tccatcggcc tgaactgcgc cctgggcgcg
     5041 gcgctgatgc gcccctatat ccaggagctg gccaaggccg cccccgatac cttcatcagc
     5101 tgctatccca acgccggcct gcccaacccc atgagcgaca ccggctttga cgagacgccc
     5161 gacgtcacca gccgcctggt gcacgagttt gccgccgagg gcctggtgaa catcctgggc
     5221 ggctgctgcg gcaccacgcc cgaccatatc gccgccatcg cccgcgccgt ggcgccggtg
     5281 ggcacgcgcc ggctgttcac cgccgagccg gcctgacggc ccaaagcggc ccctcgccca
     5341 tgcgcctggc ctgccctctt gccgccgcgt tgtgtgctgc ggttcttctc tggggctgtg
     5401 ccagcgcgcc ggcgcccgcg ccgctgccag acgcacccga cctgctgctg cgcgcgctat
     5461 gagacggatt tgcacgcctg ccgcgccggc gtgggcagtg ctggcgtgct gcgcgagacg
     5521 ctggatgtcg ccctgcaagg ggcgctggtg gccgccatgc tcgcctgggg tgcgggcggc
     5581 ggcgccgaca gcgtgcgtgg ctgggcctgg ggcggtgcgg cactggtcgg tggatcgcag
     5641 gggctgcagg cgctggagcg acgccggcag gcggtggtgc gctgcatgca ggcgcgtggc
     5701 catggcggcc cgccctacgc cgcggtggtg gcgccggcgg tggcgcgccc ccatccatcg
     5761 gtgccagtag tgtgcccgcc gcccccacca ccggggccga cggctatagc gcagaacgcc
     5821 tggcgctcgg ccaggcctgc catagccagc cgcgcgcgca actggtggcc aaagggccgg
     5881 gcttcgagac ctatcacgtg gactgcagca acggtgacgc gctgtcgatc cgctgcgaat
     5941 tcggtaactg ccgggtgctg cgctaacggc tggcggcaag ggctggtgct gttcagactg
     6001 gcctcagctt gcgccatgac gatacgggcg cggtaacgca cgcgggcacc cgctgcatgc
     6061 cgccaccagg aggctgtgat gcggtccatg ctttttcggg acatctttgc cggtttcgtg
     6121 cgcacccggc gcatcctgcg ccactaccgg cgcctggcgc tgctggagac gctgacgcgc
     6181 accggcgtca gccgcgagcc gcccgagtcg ctgacccagg ccctgaacgc cgcggcgcac
     6241 agcgacggcg cggcgctgct cactcagttg ggcagcagcg ccgccggcct gaccaagacc
     6301 caggccgacg ccgtgcgcga gcgcgtcggc cccaacgagg tggagcatga aaagcccctg
     6361 ccctggtggc tgcacctgtg gcacagcttt cgcaacccgt tcaacctgct gctgacgctg
     6421 ctggccggca tctccttcct gacgcacgac accaagggcg ccctgcttat cagcaccatg
     6481 gtggtgctgt ccacgctgct gcgcttttgg caggagcgca agtccaacca ggcggccgac
     6541 gcgctcaagg ccatggtcag caacacggcc acggtgctgc gcccggcgca agcggcgggc
     6601 gccgaggcgc ggcgcatgga ggtcgccatc aagctgctag tgcccggcga cctggtctgg
     6661 ctgtcggccg gggacatgat tcccgccgac tgccgcgtgc tcacggccaa ggacttgttc
     6721 gtctcgcagg cggccatgac cggcgagtcc atgccggtgg aaaagtcggc cggcctgggc
     6781 ccggccccgg ccggcaatgc gctggatctg gacaacctgg tcttcatggg caccaacgtg
     6841 gtctcgggct cggccgcggc ggtggtgctc tataccggca acaacagcta tttcggcgcg
     6901 ctggccagcc gcgtcatcgc caccaaccgc gcgcccacgg ccttctatgc cggcgtgaac
     6961 aaggtcagct ggctgctgat ccgcttcatg ttcgtgatgg cgccactggt gttcttcatc
     7021 aacggcttca ccaagggcga ctgggtggag gcgctgctgt tcgccctggc cgtggccgtg
     7081 ggcctgacgc ccgagatgct gcccatgatc gtcacctcga cgctggccaa gggggcggtg
     7141 ttcctgtcgc gcaagaaggt catcgtgaaa cggctggacg cgatacagaa cttcggcgcc
     7201 atggacgtgc tatgtaccga caagaccggc acgctgacgc aggacaaaat ctttttggcg
     7261 cgccatgtcg atgcctgggg cgaggagtcg ggcgaggtgc tggagctggc ctacctgaac
     7321 agctactacc agaccggcct gaagaacctg ctggacgtgg ccgtgctgga gcatgccgag
     7381 gtgtcgcgcg agatcgagcc ggcgaagaat ttccacaagg tcgatgagat cccgttcgac
     7441 ttcacgcgcc ggcgcatgtc ggtggtggtg gccgagcacg atgccagcca tctgctcatc
     7501 accaaggggg cggtcgagga gatgctgacc gtatgcaccg cggtgcgcca cggcgagacc
     7561 caggagccgc tcaccccaga gctgctcacg cgcatccgcg ccgtgacggc cgacctgaac
     7621 aaggaaggcc tgcgcgtggt ggccgtggcc gccaagcagg gcccacccac ccagcaggtc
     7681 tacggcctgg ccgacgaatg cggcctgacc ctgatcggct acgtggcctt cctcgaccca
     7741 cccaaggact ccacggccgc ggccctga
//
LOCUS       DAOMTQ010000136         7713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2151_length_7713_cov_26.286498, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000136 DAOMTQ010000000
VERSION     DAOMTQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2151_length_7713_cov_26.286498"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK518_12110"
     CDS             <1..198
                     /locus_tag="PK518_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HRM49704.1"
                     /translation="RKGVVEADKPLLKQIADLGVTVTQLSPAEREAFVKATRPVYDKW
                     KGQIGADLVGAAEKAIAARKQ"
     gene            complement(250..1173)
                     /locus_tag="PK518_12115"
     CDS             complement(250..1173)
                     /locus_tag="PK518_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49705.1"
                     /translation="MEQSPYLFFAEVVEAGSFATAARRLGMDRSNVSRRIKDLEHTAG
                     VQLLRRTTRKMVLTDLGAAFYEQCLAVRGEVEQAKSLLLSHGGVVRGALHVSCPPAIG
                     RQYLVPLFEAFCRRYPAVSLQITLKSGALDLIEERIDVAIRFTNEPSPQHVARVLGHT
                     QWIFCASPTYLAEQGTPQLPEDLAQLAWLGMRARMELQLRKESALHRVVLRSRVACPD
                     YGLLGQLARDGLGVCLLPVYVASRDLAQGELVQVLADYQIEPTPGSTLYAITLPGIHS
                     SPQVKAFVQFLAETVQAEFPPVDLKKIAASP"
     gene            complement(1251..2228)
                     /locus_tag="PK518_12120"
     CDS             complement(1251..2228)
                     /locus_tag="PK518_12120"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRM49706.1"
                     /translation="MNAITSRIPAALQNLALPIIASPMFTVSYPELVLAQCKAGIVGS
                     FPALNARQPELLGEWLHQMKADLDTYRAAHPGQPVGPIAVNQIAHSSNGRLMQDMETC
                     IQHQVPVIITSLRAPAREVVDAVHGYGGVIFHDVVNVRHAQKALEAGVDGLIVVAAGA
                     GGHAGTQSPFALVGEVRRIFDGPLALSGAMATGAAVLAARAMGADFAYMGTRFIASKE
                     ANASEDYKQAIVNAGASDIIYSNWFSGVHGNYLRESIERVGLDPENLPVGDKANMSFA
                     DGRSKAKAWKDIWGAGQGVGQIHDLPEVREIVARLRAEYDLARARICAG"
     gene            complement(2270..3256)
                     /locus_tag="PK518_12125"
     CDS             complement(2270..3256)
                     /locus_tag="PK518_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49707.1"
                     /translation="MKQQLSNLAAATLALCAAAAVTQAQAYPSKPVTIVVPYSAGGPV
                     DNFIRALSQQLSDSWKQPVVVLNKPGANEIIGADQVAKSAPDGYTLFAGTEASLTMSP
                     HLYKKLPYNVEADFVPISQLISLPLMLFTHKNTPANTVPEFVELARKAKAQGKPLSYG
                     SSGAGGIAHLPMVTFEKQEGISMTHVPYRGAANLIPDVIAGQVDAAVLAVSVIEQHVK
                     TGVLKPLAVSSETRSSALPQVPTFKEAGVKDIHAIFSIGLLAPKGTPAAVVEKVAASA
                     KAIIATPDFRQKNIDAFSYVPVASTPVEFKNYLGSNSKLQAERVKASGVTLD"
     gene            complement(3303..3764)
                     /locus_tag="PK518_12130"
     CDS             complement(3303..3764)
                     /locus_tag="PK518_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRM49708.1"
                     /translation="MTPRYFEDFTVGEHFTSSGRTVTEADLTLFNMISGDWNPIHADA
                     EFAAGTRFGQRIVHGAFGVALLTGFMHQMGIFDGTAVAMLSLREWAFKAPILIGQTLR
                     LQLTVTGLHPGSSPRVGRLGRHLQLVDQHGQVVQEGSSDLLILKRPPAQPA"
     gene            complement(3761..5392)
                     /locus_tag="PK518_12135"
     CDS             complement(3761..5392)
                     /locus_tag="PK518_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM49709.1"
                     /translation="MSDLRFSALMQARLAADPQAPAFVHGEGVTTVAALAQQVQCMTA
                     WLRSAGIGPGDRVAVWLVNRVEWLALLMAAARIGATVAAVNTRYRREEVAHILRTSGA
                     RVLVTQQPHQRLDFLAILAGLDPAHVPDLQQVALLGSGSAAPAGVADLAAGLRWPLVA
                     LDWQQCTPDAGPDESNPDATVVLFSTSGTTKAPKLVMHPQRTLVDHALRCATAYCLDA
                     PDACLLTLLPLCGAFGLNSTLAAFAAGAPVVLPALFEAEQAAALLVRHAVTHCFGSDE
                     MVRRLADSSQEAQPFPSLRFFGFGAFTSNFTEIAQACCARGIPLHGLYGSSEVLAVFS
                     AQAGDAPLAQRLEGGGVPVAGGQAHIRIRDEATGQLLPAGQSGQIEIQAPSLFQGYFR
                     NPEATAEAMYEGYFQTGDLGHLRPDGSLVFETRLGDAVRLGGNLVNPAEIEELLQRDP
                     GVREAQVVAVPIQGQDRPVAFIVPEAGAGPDTAALLARLRQSIASYKVPQRVWMLSAF
                     PLTQGANGNKISRAQLRQMAQERLAAEATEPQEAA"
     gene            complement(5553..5729)
                     /locus_tag="PK518_12140"
     CDS             complement(5553..5729)
                     /locus_tag="PK518_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49710.1"
                     /translation="MAFTQASLSDEVASQYAHDGSLVRYRLRGSECQALQAAQARGWE
                     QSCTLGRIGKNPNH"
     gene            5695..5814
                     /locus_tag="PK518_12145"
                     /pseudo
     CDS             5695..5814
                     /locus_tag="PK518_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450268.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
     gene            complement(5878..6231)
                     /locus_tag="PK518_12150"
     CDS             complement(5878..6231)
                     /locus_tag="PK518_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRM49711.1"
                     /translation="MDVNALQRQLRDFAAARDWQPYHSPKNLAMALMVEAAELLELFQ
                     WLTPQEARHLTDNAADKERVGDELADVLLYLLQLADHTGVDLEQAVAAKLAKNARKHP
                     ARHPAPPPDAAQGFE"
     gene            complement(6281..>7713)
                     /locus_tag="PK518_12155"
     CDS             complement(6281..>7713)
                     /locus_tag="PK518_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRM49712.1"
                     /translation="RFHLERQTGGMTRDIERGVRGIESMVSFALFNIVATLVEVLLVL
                     GVLAARFDIWFAVITLTALGLYIAYTVGVTEWRIQYRRQANQFDSAAHGKAIDSLLNY
                     ETVKYFNNEAFEARRYDQSLEQLRRAQLKSRSSLGLLNAGQQLIIAVGLVAMLWRATE
                     GVAAGRMTLGDLVMVNAFMIQIYIPLNFLGVIYREIKQNLTDLDKMFTLMDKEQEVAD
                     APGATDLAGLEPPTLRFEDVHFAYDPARPILRGVSFEIPAGKTVAVVGPSGAGKSTLS
                     RLLYRFYDIQQGRITIAGQDIRAVTQQSLRRAIGIVPQDTVLFNDTVAYNIAYGRTGA
                     SQAEVEQAARAAHIHGFIAAQPRGYETTVGERGLKLSGGEKQRVAIARTLLKNPPILI
                     FDEATSALDSANERAIQAELRQVAQGKTTLVIAHRLSTVVDAHEILVMEAGTIVERGT
                     HAALLAAGGRYASMWALQQSSSEMSA"
BASE COUNT         1335 a         2514 c         2574 g         1290 t
ORIGIN      
        1 cgcaagggcg tggtcgaggc cgacaagccg ctcctgaagc agatagcaga cctgggcgtg
       61 accgtgacgc agctctcgcc cgccgagcgc gaagccttcg tcaaggccac gcgcccggtg
      121 tacgacaagt ggaagggcca gatcggcgcc gacctggtgg gcgcggcaga gaaggcgatt
      181 gcggcgcgca agcagtaacc agcaagcaac ccagtagatc agaaaataat agctgccagc
      241 gcttgtacat caagggctgg cagctatttt ctttaaatcc accgggggaa attcagcctg
      301 cacggtttcc gccagaaatt ggacgaaggc cttgacctgc ggtgatgaat ggatgcctgg
      361 cagggtgatg gcatacagcg tgctgccggg cgtgggctcg atctggtagt cggccagcac
      421 ctgcactagc tctccctggg ccaaatcgcg ggacgcgaca tagaccggca gcaggcacac
      481 gcccagcccg tcgcgggcca gctggcccag caggccgtag tcagggcagg ccactcgcga
      541 gcgcagcacc acgcggtgca gggccgactc tttgcgcaac tgcagctcca tgcgggcacg
      601 catgcccagc caggccagct gcgccagatc ctcgggcagc tggggcgttc cctgctcggc
      661 cagataggtc gggctggcgc aaaaaatcca ttgcgtatgc cccagcacgc gggccacatg
      721 ctgcggcgac ggctcgttgg tgaagcgaat cgccacgtcg atgcgctctt cgatcaagtc
      781 cagcgcgccc gacttcaagg tgatctgcaa ggacaccgcg gggtagcggc gacaaaaagc
      841 ctcgaacagg ggcaccaggt actgcctgcc aatcgctggt ggacagctga catgcagcgc
      901 cccgcgcacc acgccgccgt gcgacagcag caggctcttg gcctgctcaa cctcgccgcg
      961 cacggccagg cactgctcgt agaacgcagc gcccaggtcg gtcagcacca tcttgcgggt
     1021 ggtgcggcgc agcagctgca cgcctgcggt gtgctcgagg tccttgatgc ggcggctgac
     1081 gttggagcgg tccatgccca ggcgccgcgc ggccgtggca aaactgcctg cttccacgac
     1141 ctcggcaaaa aataaatagg gcgactgttc caccggcaaa acctccggtg ggcgagcata
     1201 aggcctgcgc cctgccccgc ccggcgcgac ggtgggcaac acgggcgcgc tcaaccggcg
     1261 caaatgcggg cgcgggccag gtcgtattcg gcgcgcagcc tggccacgat ctcgcgcact
     1321 tcgggcaggt cgtggatctg gcccacgccc tggcctgcac cccagatgtc tttccaggcc
     1381 ttggccttgg atcggccatc ggcaaagctc atgttcgcct tgtcgcccac cggcaggttt
     1441 tccgggtcca ggcccacgcg ttcgatgctt tcgcgcaggt agttgccgtg cacgcccgag
     1501 aaccagttgc tgtagatgat gtccgacgcg cccgcgttca cgatggcctg cttgtagtcc
     1561 tcgctggcat tggcttcctt gctggcaata aagcgcgtgc ccatgtaggc aaagtccgcg
     1621 cccatggcgc gtgcggccag cacggcggcg ccggtggcca tggcgccgga cagggccagg
     1681 ggcccgtcaa aaatgcgccg cacctcgccc accagcgcaa acggcgactg ggtgcccgca
     1741 tggccacccg cacccgccgc cacgacgatc aggccatcga cgcccgcctc cagcgccttt
     1801 tgcgcatggc gcacattgac cacgtcatga aagatcacgc cgccgtagcc gtgcacggca
     1861 tccacgactt cgcgcgcagg cgcgcgcagc gaggtgatga tgaccggcac ctggtgctgt
     1921 atgcaggttt ccatgtcctg catcaggcgc ccgttggagc tgtgcgcaat ctggttgacc
     1981 gcaatcggcc ccaccggctg gccagggtgg gccgcccggt aagtatccag atcggccttc
     2041 atctggtgca gccactcgcc cagcagctcg ggctggcggg cgttgagcgc cgggaacgag
     2101 cccacgatgc cggccttgca ctgcgccagc accagctcgg gatagctgac ggtgaacatc
     2161 ggcgatgcga tgatgggcag cgccaggttc tgcagcgcgg cggggattcg ggacgtaatg
     2221 gcgttcatgg tcgctcgttc agaaggtcag ggaacaaaga ggggccatat cagtccagcg
     2281 tcacgcccga ggctttgacc cgctcggcct gcaacttact gttgctgccc aggtagttct
     2341 tgaactccac cggggtcgat gccacgggca cgtagctgaa ggcatcgatg tttttctggc
     2401 ggaagtccgg cgtggcgatg atggccttgg cgctggccgc caccttctcc accaccgccg
     2461 caggggtgcc cttgggcgcc agcaggccga tgctgaagat ggcgtgaatg tccttgacac
     2521 ccgcctcctt gaaggtgggc acctggggca aggccgagct gcgggtttcg gacgacactg
     2581 ccagaggctt gagtacaccg gtcttcacat gctgctcgat caccgacacg gccagaactg
     2641 cggcatccac ctggccggca atcacgtcgg gaatcagatt ggccgcgccc cggtagggca
     2701 catgggtcat ggagatgccc tcctgctttt caaaggtcac catgggcagg tgggcaatgc
     2761 cgcccgcgcc agacgagcca tagctcaacg gcttgccctg ggccttggcc ttgcgggcca
     2821 gctcgacaaa ctcgggcacg gtgttggccg gagtgttctt gtgcgtgaac aacatcagcg
     2881 gcaggctgat cagctggctg atgggcacga agtctgcttc cacgttgtag ggcagcttct
     2941 tgtagaggtg cgggctcatg gtgagcgagg cctcggtacc ggcgaacagg gtgtagccgt
     3001 ccggcgcgct cttggcgacc tggtcggcgc caatgatttc gttggcgccc ggcttgttga
     3061 gcaccaccac cggctgcttc cagctatcgg acagctgctg gctcagggcg cggatgaagt
     3121 tatccaccgg cccgcccgcg gaatacggca ccacgatggt cacgggcttg ctggggtagg
     3181 cctgggcctg ggtcaccgcc gccgcggcac acaaggccag ggtcgcggct gcaaggttgg
     3241 aaagctgttg cttcatgttg tctcctaagg atggaaaaaa ggcacctggt aaagctaaga
     3301 gatcaagcgg gttgcgcggg cgggcgcttg aggatgagta aatcgctgct gccttcttgc
     3361 accacctggc cgtgctggtc caccagttgc aaatgccggc ccaggcggcc cacgcgggga
     3421 ctgctgcccg gatgaagccc ggtcacggtg agttgcaggc gcagggtttg tccaatcagg
     3481 atgggtgcct tgaaagccca ttccctgagc gagagcatgg ccaccgccgt gccatcaaaa
     3541 attcccatct ggtgcatgaa gcccgtcagc aaggccacgc caaaggcgcc gtggacgatg
     3601 cgctggccaa agcgggtgcc ggcggcaaac tcggcgtcgg cgtggatggg gttccagtcg
     3661 cccgagatca tgttgaacag ggtgaggtcg gcttcggtca cggtgcggcc cgagctggtg
     3721 aaatgctcgc cgacggtgaa gtcttcaaag taacgggggg tcatgcggcc tcctggggct
     3781 cggtggcttc ggcggccagg cgttcctgcg ccatctggcg caattgcgca cgggaaatct
     3841 tgttgccgtt ggcgccctgg gtcagtggga aggcagagag catccacacg cgctgcggca
     3901 ccttgtagct ggcaatggac tggcgcagcc gggccagcag ggccgccgtg tccggccccg
     3961 cacccgcctc agggacgatg aaggccacgg gacggtcctg gccctggatg ggcaccgcca
     4021 ccacctgcgc ctctcgcacg ccagggtcgc gctgcaaaag ctcttcaatt tctgccgggt
     4081 tgaccaggtt gccgcccagg cgcacggcat cacccaggcg ggtctcgaac accaggctgc
     4141 catcggggcg caggtggccc aggtcgccgg tctggaaata gccttcgtac atggcctctg
     4201 ccgtggcctc cgggttgcgg aagtagccct ggaacaggct gggcgcctga atctcgatct
     4261 gccccgactg ccctgccggc agcaactggc ccgtggcttc gtcgcggatg cggatatgcg
     4321 cctggccccc ggccaccggc acgccgccgc cttccaggcg ctgcgccagc ggcgcgtcac
     4381 ccgcctgggc cgagaaaacg gccagcacct cgctggagcc gtacaggcca tgcagcggaa
     4441 tgccgcgcgc acagcaggcc tgggcaattt cggtgaagtt cgacgtaaag gcgccaaagc
     4501 caaagaagcg cagcgacgga aacggctgcg cctcctgcga gctgtccgcc aggcggcgca
     4561 ccatctcgtc gctgccaaaa caatgcgtca ccgcatggcg caccagcaag gcggcggcct
     4621 gttcggcttc aaacaaggct ggcaaaacca ctggcgcgcc agcggcgaac gcggccaggg
     4681 tgctgttcag cccgaaagca ccgcacagcg gcagcagggt cagcaggcag gcgtcgggcg
     4741 catccaggca gtaggcagtg gcacagcgca aggcatggtc caccagggtg cgctgcgggt
     4801 gcatgaccag cttgggcgcc ttggtggtgc ccgaggtgga gaacagcacc accgtggcat
     4861 cgggatttga ttcgtccggc ccggcatcgg gcgtgcattg ctgccagtcc aacgcaacca
     4921 gcggccagcg caatccggcc gcaagatccg ccacgccagc aggcgcggcg ctgccgctgc
     4981 ccagcaaggc cacttgctgc aagtcgggca catgcgcggg gtccaagcct gccaggatgg
     5041 cgagaaagtc cagccgttgg tggggctgct gcgtcaccaa cacacgggcg ccgctggtgc
     5101 gcagaatgtg ggccacctct tcccggcgat agcgggtgtt gacagcagct accgtggcgc
     5161 cgatgcgcgc cgccgccatc agcagcgcca gccattcgac ccggttcact agccacacag
     5221 ccactcggtc gcccggccct atacccgccg aacgcaacca ggccgtcatg cactgcacct
     5281 gctgcgccag cgccgccacc gtggtgacgc cctcgccatg cacaaaggcc ggcgcctgcg
     5341 ggtcggccgc caggcgggcc tgcatcagcg cagaaaaacg taaatccgac acagtctgct
     5401 ctcctccagg gactaacggg ctttggtgag caatctcgcc agcgtgcttt ctggtgcacg
     5461 gcgaacatcg attgctgttg atgccgattg tttcaggagg attgcaagcc aggaagacgg
     5521 tcaaaacaca ttggattgat gctcaagata catcaatgat tcgggttttt accaatccgg
     5581 ccgagcgtgc acgattgctc ccagccccgc gcctgggccg cttgcaaggc ctggcactcc
     5641 gagccgcgca accggtagcg gacaaggctg ccgtcgtgcg catattggct ggcgacttcg
     5701 tcagagagcg aggcctgcgt gaaggccacg cgcccggtgt acgacaagtg caagggccag
     5761 atcggcgccg acctggtggg cgcggcagag aaggcgattg cggcgcgcaa gcagtcaacc
     5821 aacgctcttg aaaaaagagc tgccaacgct ttcaaacagg gcgtttgcag ctctttttca
     5881 ttcgaagccc tgggccgcat ccggcggcgg cgcgggatgt cttgccgggt gcttgcgcgc
     5941 attcttggcg agcttggcgg ccaccgcctg ctccaggtcc acgccggtgt ggtcggccag
     6001 ctgcagcaga tagagcagca cgtcggcgag ctcgtcgccc acgcgttcct tgtcggcggc
     6061 gttgtcggtg aggtggcgcg cctcctgcgg cgtgagccac tggaacagct ccagcagttc
     6121 cgccgcctcc accatcagcg ccatggccag gttcttgggc gagtggtagg gctgccagtc
     6181 gcgcgccgcg gcgaagtcgc gcagctggcg ctgcagggcg ttcacatcca tggtggactc
     6241 acatcttttt tgatagctgc ttgcgcttta tgcataagcg ttaagcactc atttcgctcg
     6301 aactttgctg cagcgcccac atgctcgcat agcgcccgcc cgcggccagc agcgcggcgt
     6361 gcgtgccacg ctcgacgatg gtgccggcct ccatcaccag gatctcgtgc gcatcgacca
     6421 cggtggacag gcggtgcgcg atcaccagcg tggtcttgcc ctgggccacc tggcgcagct
     6481 cggcctggat ggcgcgctcg ttggccgagt ccagcgcgct ggtggcctcg tcgaagatca
     6541 ggatgggcgg gttcttcagc agcgtgcgcg cgatcgccac gcgctgcttt tcgccgccgc
     6601 tgagcttcag gccgcgctcg cccaccgtcg tctcgtagcc cctgggctgg gcggcgatga
     6661 agccgtggat atgcgcggcg cgcgcggcct gctcgacctc ggcctggctg gcgccggtgc
     6721 ggccataggc gatgttgtag gccaccgtgt cgttgaacag taccgtgtcc tgcggcacga
     6781 tgccgatggc ccggcgcagc gactgctggg tcacggcgcg gatgtcctgg ccggcgatgg
     6841 tgatacggcc ttgctggatg tcgtagaagc ggtacagcag gcggctgagc gtgctcttgc
     6901 ccgcgcccga ggggccgacc acggccaccg tcttgccggc aggaatctcg aagctcacgc
     6961 cccgcaggat gggccgcgcc gggtcgtagg cgaagtgcac gtcctcgaag cgcagcgtgg
     7021 gcggctccag ccccgccagg tcggtggcgc cgggcgcgtc ggccacctcc tgctccttgt
     7081 ccatcagcgt gaacatcttg tccaggtcgg tcaggttctg cttgatctcg cggtagatca
     7141 cgcccaggaa gttcagcggg atgtagatct ggatcatgaa ggcgttgacc atgaccaggt
     7201 cgcccagcgt catgcggccg gcggccacgc cctcggtggc gcgccagagc atggccacca
     7261 ggcccacggc aatgatgagc tgctgcccgg cgttgagcag ccccaggctg ctgcggctct
     7321 tgagctgggc gcggcgcagc tgctccaggc tctggtcgta gcgccgcgcc tcaaaggctt
     7381 cgttgttgaa gtacttgacg gtctcgtagt tcagcagcga atcgatggcc ttgccgtgcg
     7441 cggccgagtc gaattgattc gcctggcgcc ggtactggat gcgccactcc gtcacgccca
     7501 cggtgtaagc gatgtacagc cccagggcgg tgagtgtgat cacggcaaac cagatgtcga
     7561 accgggccgc cagcacgccc agcaccagca gtacctcgac cagcgtggcg acgatgttga
     7621 acagcgcgaa cgacaccatg gactcgatgc cgcgcacacc gcgctcgatg tcgcgcgtca
     7681 tgccgccggt ctggcgctcc aggtgaaagc gca
//
LOCUS       DAOMTQ010000137         7686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2164_length_7686_cov_30.687590, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000137 DAOMTQ010000000
VERSION     DAOMTQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_7686_cov_30.687590"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PK518_12160"
     CDS             complement(<1..236)
                     /locus_tag="PK518_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRM49713.1"
                     /translation="MALMDFIKKQFIDILQWTEEGDGTLAWRFPMAGMEIQTGATLVV
                     RESQMALFVNEGQVADVFGPGTYKLTTQTLPVLT"
     gene            398..700
                     /locus_tag="PK518_12165"
     CDS             398..700
                     /locus_tag="PK518_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HRM49714.1"
                     /translation="MSRIRNAARNNLPTLAKTGSYYVIHICVAAMVAYAVTGNWIASL
                     TLSLLEPTVQAVAFFFHEKAWQRAAERKAAAQASVSSSVPAMSSAVRSTSALALKA"
     gene            complement(622..945)
                     /locus_tag="PK518_12170"
     CDS             complement(622..945)
                     /locus_tag="PK518_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRM49715.1"
                     /translation="MADTRETMSSLLSIASSGLQAAQLRLNASAHNVANLNTPGFKDQ
                     TVQQQAVPPTGGVQARTAQAARPGVALEAEAVEQITASYAFKANALVLRTADDMAGTL
                     LDTLA"
     gene            complement(969..2015)
                     /locus_tag="PK518_12175"
     CDS             complement(969..2015)
                     /locus_tag="PK518_12175"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRM49716.1"
                     /translation="MMSSIRQAVSFARCLAGVLLAALAAGHAAAQDVVSNFASGPGNG
                     VYAFASSTPKTMQELMTGGGEAVNIRGHLFLPPGDGAKLPAVVLLHGSGGIYDALLDY
                     WPRQFNAAGMAVFTLDMFGPRGVQSTAEDQSAVPFVADVADSFAALRLLATHPRIDSK
                     RIALMGFSRGGSATLRANVTRIIAAQRLPDGLRYAAFIPTYAGGCVGVFRLVVRSGVF
                     TPAPMLFIHGDADDYTPIGPCLDYADKIGRAGTPVQFVTLAGARHKFDADDQRRHQMR
                     NAVRIRPDCTVETDIDTLTAHDGATGARLQGPAYRDALKACQAVGATVEGSTKARDQA
                     TQAALAFLKKVFGL"
     gene            complement(2249..3196)
                     /locus_tag="PK518_12180"
     CDS             complement(2249..3196)
                     /locus_tag="PK518_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49717.1"
                     /translation="MKRSASSTPRPRAVMGQLSDMDLRLLQVFKAVVECGGMAAAELE
                     LNIGTSTVSRHVKDLETRLGMTLCRRGRAGFALTPEGQRVYEETLRLLASVRGFLGSI
                     DDIHARMGGQLAVAVFDKTASNPAARIHAAIAAFAEQAPDVQLQLHVGSINAIERGII
                     DGGFQVGIIPAHRSSQSLDYTDLFGETMLLYCGAGHPLFGAAHDALDWDAVRRHPFAG
                     LGYHSPNMELSHQAQLERTATGFDQESIATLLLSGRFLGFLPDHYAEAFERRGRLQAV
                     RPDLFHYRCQFVSLLRRSPAPSRAALLFQQCLAQAHAAV"
     gene            complement(3206..3508)
                     /locus_tag="PK518_12185"
     CDS             complement(3206..3508)
                     /locus_tag="PK518_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM49718.1"
                     /translation="MARVQIARSAETDLLEGYVFYEQQQAGIGDYFLDSLSADIDALA
                     LYAGVHPRPDSRLHRTLSRRFPFAIYYDLKGDIATVVAVLDCRQNPASIAQRLGRP"
     gene            complement(3510..3731)
                     /locus_tag="PK518_12190"
     CDS             complement(3510..3731)
                     /locus_tag="PK518_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRM49719.1"
                     /translation="MTTINIAELPVAEKLRLMEALWDALSAQAEQAAVPAWHREVLAQ
                     RLQRLDSGTEPVTPWTQAKERIRAQVRAA"
     gene            3884..5239
                     /locus_tag="PK518_12195"
     CDS             3884..5239
                     /locus_tag="PK518_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRM49720.1"
                     /translation="MSFAVVDDNRTAIRQDAAWLDAHWMPFTGNRNFKANPRMVVGAQ
                     GAYYTDADGRKIFDGLSGLWCSGLGHGRKEVAEAIGRAAANLDYAPAFQFGHPAAFEL
                     ANRIKDLTPAGLDYVFFTGSGSEAADTSLKMARAYWRAKGQGGKTRLIGREKGYHGVN
                     FGGISVGGMVGNRKLFGQGVEADHMPHTQPPLGSFCRGMPDTDGRALADKLLDVIALH
                     DASNIAAVIVEPFSGSAGVVIPPVGYLQRLREICTQNNILLIFDEVITGFGRCGGWTG
                     AEVFGVTPDIMNFAKQVTNGAQPLGGCVAAKEIYDTFMAAGGPEYMLEFAHGYTYSAH
                     PVACAAGNAVLDILQKEDMPARVKALSPYFENAVHGLKGAKHVADIRNYGLAAGFTIA
                     SLPGEPARRPYEVAMKCWDKGFYVRYGGDTIQLAPPFISTEAEIDRLVSALGDALQET
                     A"
     gene            5360..6877
                     /locus_tag="PK518_12200"
     CDS             5360..6877
                     /locus_tag="PK518_12200"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRM49721.1"
                     /translation="MHHDQNVTATVGHLIDGQLVADTERTQPVFNPATGQSTTSVALA
                     SKATVEAAIASAEAAFPAWRATPPLKRARVMSRLKVLLEENADKIAAMITAEHGKVLS
                     DAHGEIQRGIENVEYASYAPELLKGEHSRNVGPGIDSWAEHQALGVTAGITPFNFPAM
                     VPLWMWPMAVACGNTFVLKPSERDPSSTLFIAQLALEAGLPPGVLNVVNGDKTAVDTL
                     LQDPRVKAVSFVGSTPIAEYIYSEGCKHGKRVQALGGAKNHAVLMPDADVANAVNALM
                     GAAYGSCGERCMAIPLLVAVGDEVGDAVVAGLKAEIARMKVGPGTDNGNDMGPLVTRQ
                     HFEKVKAYVDSGVAEGAQLVVDGRGVQVAGHEDGYFLGACLFDNVKPGMKIYQEEIFG
                     PVLGVVRVKTLQEAMALIDAHEYGNGTCIFTRDGEAARYFTDHIQVGMVGVNVPLPVP
                     VAYHSFGGWKRSLFGDLHAYGPDAVRFYTKRKTITQRWPSAGVREGAVFSFPSSR"
     gene            complement(6954..>7686)
                     /locus_tag="PK518_12205"
     CDS             complement(6954..>7686)
                     /locus_tag="PK518_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRM49722.1"
                     /translation="KAPPDGYTVILGDVGCMSMAPGLYSRLPYDPLRDFAPVSLVGRS
                     PLVLTVGTQSPLQSLQDLTAAAQAAPGKLNYPSSGTGGPNHLGAELYAMQAKVKVSHI
                     PYKGSAPSVVSLVAGETDFGFLTAVTIASQLNAGKVRALAVAHTERLPAMPNVPTMSE
                     AGLKGFTADAWFLAAVPAGTPKPIVDRLYDEIAKALPAPDVKAKLDSMGVLATGMDPK
                     ASAQFLGSEVEKWRGVIKTAGITLD"
BASE COUNT         1339 a         2543 c         2578 g         1226 t
ORIGIN      
        1 taggtcagca ccggcagggt ctgggtggtg agcttgtagg tgccggggcc gaagacgtcg
       61 gccacctggc cctcgttgac gaacagcgcc atctgcgact cgcgcaccac cagggtggcg
      121 cccgtctgga tctccatgcc cgccatgggg aagcgccagg ccagcgtccc gtcgccctct
      181 tcggtccact ggaggatgtc gatgaactgc ttcttgatga agtccatgag ggccatggtc
      241 gtgctcccgt tcgggtctgg aggggggtga aaaaacgagc ggccatcata gcggccgcac
      301 ccggcacgcc agggccgcta tccgcgggat agccataagc cattggcgaa ttaaatgaga
      361 atgactatca tttattagac caaacaagga gtgcaccatg tcccgcatcc gcaacgccgc
      421 ccgcaacaac ctgcctacgc tggccaagac cggcagttac tacgtcatcc acatctgcgt
      481 ggccgccatg gtggcctatg ccgtcacggg caactggatc gcctcgctca cgctgagcct
      541 gctggagccc acggtgcagg cggtggcgtt cttcttccat gaaaaggcgt ggcagcgggc
      601 ggcagagcgc aaggcggccg ctcaggccag cgtatccagc agcgtgcccg ccatgtcgtc
      661 ggccgtgcgc agcaccagcg cattggcctt gaaggcgtaa gaggcggtga tttgctccac
      721 ggcctcggcc tccagcgcca cgccgggccg cgccgcctgg gccgtccgcg cctgcacgcc
      781 gcccgttggc ggcacggcct gctgctgcac cgtctgatcc ttgaaaccgg gggtattcag
      841 gttggcgacg ttgtgggcgc tggcattcag gcgcagctgc gcggcctgca ggccggatga
      901 ggcgatggac agtagcgagg acatggtctc cctggtatcg gccatggggc ggccaactgt
      961 aggcccggct acagcccaaa caccttcttc aggaacgcca gcgccgcctg cgtcgcctgg
     1021 tcacgggcct tggtgctgcc ctcgacggtg gcgcccacgg cctggcaggc cttgagcgcg
     1081 tcgcggtagg ccggcccctg caggcgcgcc cccgtggcgc cgtcatgggc ggtcagggta
     1141 tcgatatcgg tctcgaccgt gcagtccggc ctgatgcgca ccgcgttgcg catctgatgg
     1201 cggcgttgat cgtcggcgtc gaacttgtgc ctcgcgccgg caagggtgac gaactgcacc
     1261 ggcgtgccgg ccctgccgat cttgtcggca tagtccaggc aggggccgat gggggtgtag
     1321 tcatcggcat ctccgtggat gaacagcatc ggtgccggcg taaacacgcc cgacctgacc
     1381 accaggcgga acacgcccac gcaaccgccg gcataggtgg gtatgaaggc agcgtagcgc
     1441 aggccgtcgg gcagcctttg cgcggcgatg atgcgcgtca cgttggcgcg cagggtggcg
     1501 ctgccgccac gcgaaaaccc catcagggcg atgcgtttgg agtctatgcg cgggtgcgtg
     1561 gccagcagcc ggagggccgc gaagctgtcg gccacgtcgg ccacgaacgg cacggcggac
     1621 tgatcctcgg ccgtgctctg cacgccgcgc ggaccaaaca tgtcgagcgt gaagaccgcc
     1681 atgccggcgg cgttgaactg cctgggccag taatcgagca aggcatcgta gatgccgccc
     1741 gagccgtgca gcagcaccac ggcgggcagc ttggcgccat cgcccggcgg caggaacagg
     1801 tggccgcgga tgttgaccgc ctcgccgccg cccgtcatca gctcctgcat ggtcttgggg
     1861 gtcgagctgg caaaggcata gacaccattg ccggggccgg atgcgaagtt gctcaccaca
     1921 tcctgggccg ccgcatggcc tgcggcgagc gctgcgagca gcacgcccgc cagacaacgc
     1981 gcaaatgaaa ccgcttgtcg aatgctggac atcatgatct gtcctttccg tggcaggagc
     2041 gccggggaga aatgctagta cagcaacctg gaagtagagg aacaaaatga aagcgatgga
     2101 ttcgcgccca gggcaaggcg caaaccgcag cgatagccgt agctatcgcg aggatttgca
     2161 acgccgccat gggtgcgaag gcgcgctttc atgtttcgat atttccgggt tgctgtacta
     2221 gacctcgccg tgccgctgtc aatgccgctt acaccgccgc atgcgcctgt gccaggcatt
     2281 gctggaacag cagcgccgcc cggctgggcg cgggcgagcg gcgcagcagg ctgacgaact
     2341 ggcagcggta atggaacaga tccggccgca cggcctgaag ccggccgcgc cgctcgaagg
     2401 cctcggcata gtggtcgggc agaaagccca gaaagcgccc ggacagcagc agcgtggcga
     2461 tggattcctg gtcgaaaccg gtggcggtgc gctcaagctg cgcctggtgc gacagctcca
     2521 tgttgggcga gtggtagccc aggccggcga aggggtggcg gcgcacggcg tcccagtcca
     2581 gcgcgtcatg cgccgcccca aacaaagggt ggccggcgcc gcagtacagc agcatggttt
     2641 cgccaaacag gtcggtgtag tcaaggcttt gcgagctgcg gtgcgccggg atgatgccca
     2701 cctgaaagcc gccgtcgatg atgccgcgct ctatggcgtt gattgagccc acatgcagct
     2761 gcagctgcac gtcgggcgcc tgctcggcaa aggcggcaat ggcggcgtgg atgcgcgcgg
     2821 ccgggttgct ggcggtcttg tcgaacaccg ccacggccag ctgcccgccc atgcgcgcgt
     2881 ggatgtcgtc gatgctgccc agaaagccgc gcacgctggc caagaggcgc agcgtctcct
     2941 cgtacacacg ctggccctcg ggcgtcaggg caaagccggc ccggccgcgg cggcacaggg
     3001 tcatgcccag gcgtgtctcc agatccttca catggcggct gacggtgctg gtgccgatgt
     3061 tcagctccag ctcggccgcc gccatgccgc cgcattcgac cacggccttg aagacctgca
     3121 gcaggcgcag gtccatgtcg gatagctggc ccatgacggc gcgtgggcgg ggggtagagg
     3181 aagcagaccg cttcatgacc accgattacg gtcgcccgag ccgctgagcg atggacgccg
     3241 ggttctggcg gcaatcgagc acggccacga ccgtggcgat gtcgcctttc aggtcgtagt
     3301 aaatggcgaa aggaaagcgc ctggatagtg tgcggtgcag gcggctatcg ggtctggggt
     3361 gaacacccgc gtacaaggcg agtgcatcga tgtctgcgga caggctgtcg agaaaatagt
     3421 cgccgatgcc cgcctgctgc tgttcgtaga acacatagcc ttccaggaga tcggtttccg
     3481 ccgaccgggc aatctgcacc cgcgccacgt cacgccgccc tgacctgcgc gcgaatgcgt
     3541 tccttggcct gcgtccaggg tgtgacgggt tcggtgccgc tgtccaggcg ctgcaggcgc
     3601 tgggccagca cttcccggtg ccaggcggga acggcggcct gctcggcctg tgcgctcagg
     3661 gcatcccaca gcgcttccat gagccgcaac ttttcggcca cgggcagttc ggcgatgttg
     3721 atggtggtca tggcggctcc agtgcgtcaa ccgaggaatg ttagcccaac tagtacgggg
     3781 atggtttttc ttgcatgttc aagcaagtaa acgttgatat ttgatacttt taagaattat
     3841 cgtcctggcc aacaatcgct gcattcattc ataggagacc gccatgtcct ttgccgtcgt
     3901 tgacgacaac cgcaccgcca tccgccagga cgccgcctgg ctcgacgccc actggatgcc
     3961 gttcaccggc aaccgcaact tcaaggcgaa tccgcgcatg gtggtcggcg cccagggcgc
     4021 ctactacacc gatgccgacg ggcgcaagat ttttgacggc ctgtcgggcc tgtggtgctc
     4081 gggcctgggc catggccgca aggaagtggc cgaggccatc ggccgcgccg ccgccaacct
     4141 ggactacgcc ccggccttcc agtttggcca cccggccgca ttcgaactgg ccaaccgcat
     4201 caaggacctg acgcccgccg ggctggacta tgtgttcttc accggatccg gctccgaggc
     4261 ggccgacact tcgctgaaga tggcacgcgc ctactggcgc gccaagggcc agggcggcaa
     4321 gacgcggctg atcggccgcg agaagggcta ccatggcgtg aattttggcg gcatctcggt
     4381 ggggggcatg gtgggcaacc gcaagctgtt tggccagggc gtggaggccg accacatgcc
     4441 ccacacgcag ccgccgctgg gctcgttctg ccgtggcatg cccgataccg acggccgtgc
     4501 tctggccgac aagctgctgg acgtgattgc gctgcacgac gcaagcaaca tcgccgccgt
     4561 gatcgtcgag ccgttcagcg gatcggccgg cgtcgtcatt ccacccgtcg gttatctgca
     4621 gcgcctgcgc gagatctgca cgcagaacaa catcctgctc atcttcgacg aggtcatcac
     4681 cggctttggc cgctgcggcg gctggacggg cgccgaggtg ttcggcgtga cgcccgacat
     4741 catgaacttt gccaagcagg tgaccaacgg cgcccagccg ctgggcggct gcgttgcagc
     4801 caaagaaatc tacgacacct tcatggccgc gggcgggccc gagtacatgc tggagtttgc
     4861 ccacggctac acctactcgg cccaccccgt ggcctgcgcg gcgggcaacg cggtgctgga
     4921 catcctgcag aaggaagaca tgccggcgcg cgtcaaggcg ctgtcgccgt attttgagaa
     4981 cgccgtgcat ggcctcaagg gtgcgaagca tgtggccgac atccgcaact acggcctggc
     5041 cgcgggcttc accatcgcca gcctgcccgg cgagccggcg cgccgcccct acgaggtggc
     5101 aatgaagtgc tgggacaagg gcttctacgt gcgctacggc ggcgacacga ttcagctggc
     5161 cccgcccttc atcagtaccg aggccgagat cgaccgcctg gtcagcgctc tgggcgacgc
     5221 actgcaagag accgcctgaa cctgggagcg ccggcgtccc gccggctctc catgctccac
     5281 gcaccataaa aacaatagct atcagcgctt accagataag cgctacagcc actttttatc
     5341 aaattttcaa cagcacccca tgcaccacga ccagaacgtc accgccaccg taggccacct
     5401 cattgacggc cagctcgtcg ccgacaccga gcgcacccag cccgtgttca accccgccac
     5461 cggccagtcg accaccagcg tggcgctggc gagcaaggcc acggtggagg cggccatcgc
     5521 cagcgccgag gccgccttcc ccgcctggcg tgccacgcca ccgctcaagc gcgcgcgggt
     5581 catgagccgg ctgaaagtgc tgctcgaaga aaacgccgac aagatcgccg ccatgatcac
     5641 cgccgagcac ggcaaggtgc tgtccgacgc gcacggcgag atccagcgcg gcatagaaaa
     5701 cgtggaatac gccagctacg cccccgagct gctcaagggc gagcacagcc gcaacgtcgg
     5761 ccccggcata gacagctggg ccgagcacca ggcgctgggc gtcacggccg gcatcacgcc
     5821 gttcaacttt ccggccatgg tgccgctgtg gatgtggccc atggccgtgg cctgtggcaa
     5881 cacctttgtc ttgaagccct ccgaacgcga cccgagcagc accctgttca tcgcccagct
     5941 ggcgctggaa gccggtctgc cacccggcgt gctcaacgtc gtcaacggcg acaagaccgc
     6001 cgtcgatacg ctgctgcaag acccgcgcgt gaaggcggtg agctttgtcg gctcgacgcc
     6061 gatcgccgaa tacatctatt cggaaggctg caagcatggc aagcgcgtgc aggccctggg
     6121 cggcgccaag aaccacgccg tgctgatgcc cgacgccgac gtggccaacg ccgtcaacgc
     6181 gctgatgggc gcggcctacg gcagctgcgg cgagcgctgc atggccatcc cgctgctggt
     6241 ggccgtgggc gacgaagtag gcgacgccgt ggtggccggc ctgaaggccg agatcgccag
     6301 gatgaaggtc ggccccggca cggacaacgg caacgacatg ggcccgctgg tcacgcgcca
     6361 gcactttgag aaggtcaagg cctatgtgga cagcggcgtg gccgaaggcg cgcagctggt
     6421 ggtggacggc cgcggcgtgc aggtggcggg acatgaggac ggctacttcc tcggcgcctg
     6481 cctgttcgac aacgtcaaac ccggcatgaa gatctaccag gaggaaatct tcggccccgt
     6541 gctcggcgtg gtgcgcgtca agacgctgca agaggccatg gcgctgatcg acgcgcacga
     6601 gtacggcaac ggcacctgca tcttcacgcg cgacggcgag gccgcgcgct acttcaccga
     6661 ccacatccag gtcggcatgg tgggcgtgaa cgtgcccctg cccgtgcccg tggcctacca
     6721 ctcgttcggc ggctggaagc gcagcctgtt cggcgacctg cacgcctacg gcccggacgc
     6781 cgtgcgcttc tacaccaagc gcaagaccat cacccagcgc tggcccagcg ccggcgtgcg
     6841 cgagggcgcg gtgttcagtt ttccgagcag ccgctgacgg accctgatag gcgctgaaag
     6901 cacaaagccc ccgggccgca aggtccgggg gctttgttat tggtgcgcgg ctatcagtcg
     6961 agcgtgatac cggcggtctt gatcacgccg cgccacttct ccacctcgct gcccagaaac
     7021 tgggccgatg ccttgggatc catgcccgtg gccagcacgc ccatggagtc gagcttggcc
     7081 ttcacgtcgg gcgcgggcag ggccttggcg atctcgtcat acaggcgatc gacgatgggc
     7141 ttgggtgtgc ccgccggcac ggcggccagg aaccaggcat cggccgtgaa gcccttgagg
     7201 ccggcctccg acatggtggg cacgttgggc atggccggca ggcgctcggt gtgcgccacg
     7261 gccagcgcgc gcaccttgcc ggcgttgagc tgcgaggcga tcgtcaccgc cgtgagaaag
     7321 ccgaaatccg tctcacccgc caccagcgac accaccgacg gcgcgctgcc cttgtacggt
     7381 atgtggctga ccttgacctt ggcctgcatg gcgtacagct ccgcccccag atggttggga
     7441 ccgcccgtgc cggacgaggg gtagttgagc ttgcccggcg cagcctgcgc cgcggccgtc
     7501 aggtcttgga gcgactgcag cgggctttgc gtgcccacgg tcagcaccag cgggctgcgc
     7561 ccgaccaggc tcacgggcgc gaagtcgcgc agcgggtcgt agggcaagcg ggagtacaga
     7621 cccggcgcca tggacatgca gcccacatcg cccaggatga cggtgtagcc gtccggtggc
     7681 gccttg
//
LOCUS       DAOMTQ010000138         7525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2233_length_7525_cov_26.174565, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000138 DAOMTQ010000000
VERSION     DAOMTQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_7525_cov_26.174565"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..543)
                     /locus_tag="PK518_12210"
     CDS             complement(109..543)
                     /locus_tag="PK518_12210"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HRM49723.1"
                     /translation="MTKTFAITLFAASCLMGGLAQAQSTSVLLVEFQGPGGHSSGNYG
                     RTSALHAAARAVIELQKSMPTGSYSVAYLSGGNSVNSIASDGSFAVRLSASAGMTAAD
                     FLAKVALAAKSGADAENAFRNVKPGDLTAGAPADIRYSVKQQ"
     gene            complement(555..2303)
                     /locus_tag="PK518_12215"
     CDS             complement(555..2303)
                     /locus_tag="PK518_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HRM49724.1"
                     /translation="MKPHHVPSSAPLLATVSAAVLLAACGGNSDGDAEAPLPKPTAIV
                     NGVPEIDPAIAESAKKVMADKKLQDIVAELSTAASEKARFNQHMELVRIASPSRYENR
                     MAAEVHRRMVNEWGYLASEVKTRADGNLPGSDVQIVDGLPVYNACVEIKGSYSSSPNA
                     QSYKGQFPKVLVESHIDVVNPEVLPPDSAPYQPIKLQSLKDAVVTSPEQLAAIGTELA
                     FDGSGRIIEDANYALASRWFATEAAAKDGGGVRIYVPGYSDAMGNTSSLFTLARLFKK
                     NNIKPVYDLWICGTAGEEGKGNLAGMKQLFGFDQNLGTGSNPLNFVANFGSEGGGAVN
                     LTGSHRFEMKFKAPTHPAAGQPSAVEAMAATIARIADVKTPSELRPGVVKTTYTVGQA
                     SCEAPAAGSDVVPSCTLLVDMRSERTDTLNEVRASIEPLFKAGLNAENARYGKAENAV
                     DAATLEQVWYGLRPAFVNGDQANIALQAGWVTAQIVDVDKQAKVPTGSSSLNDNVPAN
                     TGVPTYNWTLASNAAGGGGHAFWEWGTKGDPKLEVQRLKRAMTAILTTSGYHLSDGTV
                     VAPSVAPIGKRSRELN"
     gene            complement(2476..4275)
                     /locus_tag="PK518_12220"
     CDS             complement(2476..4275)
                     /locus_tag="PK518_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HRM49725.1"
                     /translation="MPSASTATATAAIHYRIDPAQTHARLYQVTLTVAAPAAGQELSL
                     PVWIAGSYLVREFSKNLQSLRASQGDKAVALAQLDKHRWLAQCQSGTPLVLTYEVAAY
                     DNSVRTAWLDGARGFFNATSLCLRVLGQEDEAHVLEVLQPPALAHWSLATGLAPEQVN
                     AAGFGRYRARDYDELADCPVEMGAFWSASFSACGVPHRFIVAGAAPSFDGARLVRDAQ
                     RICEEAIRFWHGEERPPFDGYLFMLNAVHDGYGGLEHRNSTALICARRDLPRLGQSDK
                     EIQASEGYTTLLGLISHEYFHTWNVKRLRPAEFARYDYARENYTELLWFFEGFTSYYD
                     DLLLRRAGLLDHAGYLKLLTKTINQVLQTPGRRVQSVAQASFDAWVKYYRQDENTPNA
                     TVSYYTKGALVALCLDLALRRDGRTQLDEVMRALWQRCDGGPMAEADLLAVLQQLSGR
                     DWAGEIAQWVHGTEELPLAELLGAHGITLQAEPAQTAQRLGLRVAENGGIQVKTVLHG
                     GAAEAAGFMAGDEWLALEAQGQAWRLHRLDELALYAGGATEVTAIVARDRRLLRLPLA
                     LPAAGPSADTVRLDVQDAAAAQRWLGAAQAATA"
     gene            complement(4412..5131)
                     /locus_tag="PK518_12225"
     CDS             complement(4412..5131)
                     /locus_tag="PK518_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HRM49726.1"
                     /translation="MKLVPTLLAASALCLGLNALAQDSTIRKTLAERIPQLQQIDEVR
                     PTPMKGLYEVRIGTDLFYTDAQGNYLIQGELIDTKARRNLTEDRLNKLTAVDFQALPL
                     QDAFTIVHGKGERKLAVFEDPNCGYCKRFEKDMQNVDNVTVYLFLYPILSPDSAEKSR
                     NIWCAKDRAGAWQDFMLRDKTPAAASCDTAALQRNLALGRKHKITGTPTLIFADGSRV
                     PGAVGAQEVEKRLAEAGNGTK"
     gene            complement(5161..6288)
                     /locus_tag="PK518_12230"
     CDS             complement(5161..6288)
                     /locus_tag="PK518_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRM49727.1"
                     /translation="MAQTFDVCIRGAGVVGRTLALLLARERLRVALVTPPAGAPQVAD
                     VRAYALNAASRALLESVRAWPDEAHATAVLQMQVQADQDGMVRFDAAQQGCDALTWIV
                     DVPALQERLQEAIRYQPLVEQVTEPVAAALTVVCEGRASRTRAEFGVEFDITPYAQHA
                     IATRLACERPHGQVARQWFAPDGSILAFLPLGGAQGNSVAVVWSVPQEHVAHWLQADE
                     QEFTQHLHEASRGALGALQQSGPRASWPLQQAQARHWCGALPGQPGRGWALAGDAAHN
                     VHPLAGQGLNLGLGDAGALARVLHQRAPWRGVGDLRLLRRYERERKAALLPMGMAMDG
                     LQQLFARRESALAALRNWGMKGFEHSGPLKAWVARRAMGTD"
     gene            6433..>7525
                     /gene="ychF"
                     /locus_tag="PK518_12235"
     CDS             6433..>7525
                     /gene="ychF"
                     /locus_tag="PK518_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRM49728.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNTGVV
                     EVPDPRLAQLAGIITPERVVPAIVEFVDIAGLVAGASKGEGLGNQFLAHIRETDAIVN
                     VVRCFEDPNVIHVAGKVDPISDIEVIQTELCLADLATVEKALNRYSKAAKSGNDKEAA
                     KLVSLLTPIQAALNEGKPARAIAVSKEDAPLLKQFCLITAKPAMFVGNVAEDGFENNP
                     LLDKLKEYAAAQNAPVVAICAKIEAEMAEMSDEDRDMFLAEMGQDEPGLNRLIRAGFK
                     LLGLQTYFTAGVKEVRAWTIHVGDTAPQAAGVIHGDFERGFIRAQTIAFDDFIQFKGE
                     QGAKDAGKMRAEGKEYVVKDGDVMNFLFNV"
BASE COUNT         1140 a         2473 c         2614 g         1298 t
ORIGIN      
        1 accaagctac caagctgcca agatcaattg ctgttgtccc cggcccgtga aagcaaaggc
       61 acagacccgg ggtcgggtcc gtgcctccat gggagacgcg ccttgcgctt actgttgctt
      121 gacgctgtag cggatgtccg ccggcgcgcc cgccgtcagg tcaccgggct tgacattgcg
      181 gaaggcgttc tcggcatcgg cgccgctttt cgcggccaag gcgaccttgg ccaggaagtc
      241 tgccgccgtc atgccggcgc tggcgctgag cctgaccgcg aagctgccat ccgaggcaat
      301 gctgttgacc gaattgccgc cgctcaggta ggccaccgaa taggagcccg tgggcatgct
      361 tttctgtagc tcgatgacgg cgcgggcggc ggcatgcagg gcgctggtac gcccgtagtt
      421 gccgctgctg tgcccgcccg ggccctggaa ttcgaccagg aggacggagg tgctttgcgc
      481 ctgcgccagg ccgcccatca ggcaggaggc ggcgaacagg gtgattgcga aggtcttggt
      541 catggtgtca cctcttagtt gagttcgcgg ctgcgcttgc cgatgggggc caccgagggg
      601 gcgaccaccg tgccgtcgga caggtggtag ccggaggtgg tcaggatggc ggtcatggcg
      661 cgcttcaggc gctgcacctc cagcttgggg tcgcccttgg tgccccattc ccagaaggca
      721 tggccaccgc cgcccgcggc gttggaggcc agggtccagt tgtaggtggg tacgccggtg
      781 ttggcaggaa cgttgtcgtt caggctgctg gagcccgtgg gcaccttggc ctgcttgtcc
      841 acgtcgacga tctgcgccgt gacccagccc gcctgcagcg cgatgttggc ctggtcgcca
      901 ttgacgaacg ccgggcgcag gccgtaccag acctgctcca gcgtcgcggc gtcgacggcg
      961 ttctcggcct tgccgtagcg tgcgttttcg gcattcaggc cggccttgaa caggggttct
     1021 atgctggcac gcacctcgtt gagggtgtcg gtacgctccg agcgcatgtc caccagcagg
     1081 gtgcagctgg gcactacatc gctgcccgcg gccggtgctt cgcagctggc ctggcccacg
     1141 gtgtaggtgg tcttgaccac gcccgggcgc agctcggacg gcgtcttgac atcggcaatc
     1201 ctggcaatgg tggcggccat ggcctcgacc gcgctgggct ggcccgccgc ggggtgggtg
     1261 ggcgccttga acttcatctc gaagcggtgg ctgccggtca ggttgacggc gccgccgccc
     1321 tcggagccaa agttggccac gaagttgagc gggttggagc ccgtccccag gttttggtca
     1381 aagccgaaca gctgcttcat gccggccagg ttgcccttgc cttcttcgcc ggccgtgccg
     1441 cagatccata ggtcgtacac cggcttgatg ttgttcttct tgaacaggcg tgccagcgtg
     1501 aacaggctgg aggtgttgcc catggcgtct gaatagcccg gcacgtagat gcgcacccca
     1561 ccgccgtcct tggccgcggc ctcggtggca aaccagcggc tggccagcgc gtagttggcg
     1621 tcctcgatga tgcggccgga gccgtcaaac gccagctcgg tgccgatggc ggccagctgc
     1681 tcgggtgagg tcaccacggc atccttcagc gactgcagct tgatcggctg gtagggggcg
     1741 ctgtcggggg gcagcacctc ggggttgacc acgtcgatgt gcgattcgac cagcaccttg
     1801 gggaactggc ccttgtagga ctgggcgttg ggcgagctgg agtagctgcc cttgatctcc
     1861 acgcaggcgt tgtagaccgg caggccgtcg acgatttgca cgtccgagcc tggcaggttg
     1921 ccgtcggcgc gggtcttgac ctcggatgcc agatagcccc attcattgac catgcgccgg
     1981 tgcacctcgg cggccatgcg gttttcgtag cgcgagggcg aggcaatgcg caccagttcc
     2041 atgtgctggt tgaagcgcgc cttctcgctg gcagcggtgc tcagctcggc cacgatgtct
     2101 tgcagctttt tgtctgccat gacctttttg gccgactcgg cgatcgcggg gtcgatctcg
     2161 ggcacgccgt tgacgatggc cgtgggcttg ggcagcggtg cttcggcgtc gccatcactg
     2221 ttgccgccgc aggcggccag cagcacggcg gccgacacgg tcgccagcag cggtgcggaa
     2281 ctcgggacat ggtggggttt catcgtgcac tgcctcctgg atcatggttc aaattgaaac
     2341 ggacacgggg agatgcttga tgtgtggcac ccgcgtaaca aggccaccgc atgaagcggg
     2401 tgcaaactta caatgattgc tgcagtctgt cgcgccagct ggcgccgcca cgcggcggtg
     2461 gcttgtggcc tggcgctatg cggtggccgc ctgggcggcg cccagccagc gctgtgccgc
     2521 ggcggcgtcc tgcacgtcca ggcgcacggt gtctgccgat gggccggcgg caggcagggc
     2581 cagcggcagg cgcagcaggc gccggtcgcg ggcgacgatg gccgtgacct cggtggcgcc
     2641 gccggcatag agcgccagtt cgtccagccg gtgcaggcgc caggcctggc cctgggcctc
     2701 cagcgccagc cattcgtcgc cggccatgaa gcccgccgcc tcggccgcgc cgccgtggag
     2761 cacggtcttg acctgtatgc cgccgttttc tgccacgcgc aggcccaggc gctgggcggt
     2821 ctgcgcgggc tcggcctgca gcgtgatgcc gtgggcgccg agcagctcgg ccagcggcag
     2881 ctcttcggtg ccatgcaccc attgcgcgat ctcgcccgcc cagtcacggc ccgagagctg
     2941 ctgcagcacg gccagcagat cggcctcggc catggggccg ccatcgcagc gttgccacag
     3001 cgcgcgcatc acctcgtcca gctgggtgcg gccgtcccgg cgcagcgcca gatccaggca
     3061 cagggcgacg agcgcgccct tggtgtagta gctgaccgtg gcgttgggcg tgttctcgtc
     3121 ctggcggtag tacttgaccc aggcgtcaaa actggcctgg gccacggact gcacccggcg
     3181 cccgggggtc tgcagcacct ggttgatggt cttggtcagc agcttcaggt agccggcgtg
     3241 gtccagcagg ccggcgcggc gcagcagcag gtcgtcgtag tagctggtga agccctcgaa
     3301 gaaccagagc agctcggtgt agttctcgcg cgcatagtcg tagcgcgcga actcggccgg
     3361 gcgcaggcgc ttgacgttcc aggtgtgaaa gtactcgtgg ctgatcaggc ccagcagggt
     3421 ggtgtagccc tcgctggcct ggatctcctt gtcgctctga cccaggcgcg gcaggtcgcg
     3481 ccgggcgcag atcagcgccg tgctgttgcg gtgctccagg ccgccgtagc cgtcgtgcac
     3541 ggcgttgagc atgaacaggt agccgtcgaa tggcgggcgc tcctcgccat gccagaagcg
     3601 gatggcctct tcgcagatgc gctgcgcgtc gcgcaccagc cgcgcgccgt cgaacgaggg
     3661 cgcggcgccg gcgacgatga agcgatgtgg cacgccgcag gcgctgaagc tggcgctcca
     3721 gaacgcgccc atttccaccg ggcagtcggc cagctcgtcg tagtcacgcg cgcggtagcg
     3781 gccaaagccg gcggcgttga cctgctcggg cgccaggccc gtagccagcg accagtgggc
     3841 cagggcgggt ggttgcagca cctccagcac atgcgcctca tcctcctggc ccagcacgcg
     3901 caggcacagg ctggtggcgt tgaagaagcc gcgcgctccg tccagccagg cggtgcgcac
     3961 cgagttgtcg taggcggcga cctcgtaggt cagcaccagg ggcgtgccgc tctggcattg
     4021 cgccagccag cggtgtttgt cgagctgagc gagcgcgacc gccttgtcgc cctggctggc
     4081 gcgcagcgac tgcaggttct tggagaactc gcgcaccagg tagctgccgg ctatccacac
     4141 gggcagcgac agctcctggc cggcggccgg cgcggccacg gtcagcgtga cctggtacag
     4201 gcgcgcatgg gtttgcgcgg ggtcgatgcg gtagtggatg gcggctgtgg cggtggcggt
     4261 ggaggcggag ggcattgcgg aatctataaa aaagatagct gtttgcgctt ggtattcatc
     4321 gttttcagtt taataactta tgaaagcgac ctgaatacaa gcgcaaacag ctcatgtatc
     4381 aatagcaaac ccggcgcatt gccggtagcg cttacttggt gccgttgccg gcctcggcca
     4441 ggcgcttttc cacctcttgc gcacccacgg cgccgggcac gcgcgaaccg tcggcaaaga
     4501 tcagcgtggg cgtgccggtg atcttgtgct tgcgtcccag cgccaggttg cgctgcagcg
     4561 cggccgtgtc gcagctggcg gcggccgggg tcttgtcgcg cagcatgaag tcctgccagg
     4621 cgccggcccg gtccttggcg caccagatgt tgcgcgactt ctcggccgag tcggggctga
     4681 ggatggggta gaggaacagg tagacggtga cgttgtccac gttctgcatg tctttctcga
     4741 agcgcttgca gtagccgcag ttggggtctt caaacaccgc cagcttgcgc tcgcccttgc
     4801 catgcacgat ggtgaaggcg tcctgcagcg gcagggcctg gaagtccacg gcggtgagtt
     4861 tgttcaggcg atcctcggtc aggttgcgcc tcgccttggt gtcgatcagc tcgccctgga
     4921 tcaggtagtt gccctgggcg tcggtgtaga acaggtccgt gcctatgcgc acctcgtaca
     4981 gccccttcat gggcgtgggg cgcacctcgt cgatctgctg cagctgcgga atgcgctcgg
     5041 ccagggtctt gcgtatcgtg ctgtcctgcg ccaatgcgtt caagcccagg cacagggcgc
     5101 tggcggccag cagggtgggg acaagtttca ttgcgtcgtt ccgttctttc atctcattgt
     5161 tcaatcggtg cccatggcgc ggcgcgccac ccaggctttc agcgggccgc tgtgctcaaa
     5221 gcccttcatg ccccagttgc gcagcgcggc cagcgcgctc tcgcgccggg caaacagttg
     5281 ctgcaggcca tccatggcca tgcccatggg cagcagcgcg gccttgcgtt cgcgttcgta
     5341 gcggcgcagc aggcgcagat cgcccacacc gcgccacggt gcgcgctggt gcagcacccg
     5401 cgccagcgcg ccggcatcgc ccagccccag gttcaggccc tgacccgcca gcggatgcac
     5461 gttgtgcgcc gcgtcaccgg ccagcgccca cccccggccg ggctggccgg gcagggcgcc
     5521 gcaccagtgg cgtgcctgcg cctgctgcag cggccagctg gcgcgcggcc cgctttgttg
     5581 cagcgcgccc agggcgccgc ggctggcctc gtgcagatgc tgcgtgaact cttgctcgtc
     5641 ggcctgcagc caatgcgcca cgtgctcctg tggaacagac cagaccacag ccacggagtt
     5701 cccctgcgct ccgcccagcg gcaaaaacgc caggatggag ccatcgggcg caaaccactg
     5761 gcgtgccacc tggccatgcg ggcgctcgca cgccaggcgc gtggcgatgg cgtgctgggc
     5821 gtagggggtg atgtcgaact ccacgccgaa ctcggcgcgc gtgcggctgg cgcggccctc
     5881 gcagaccacg gtcagcgccg cggccacggg ttcggtgacc tgctcgacca gcggctggta
     5941 gcggatggcc tcctgcagcc gctcctgcag cgccggtaca tccacgatcc aggtcagcgc
     6001 gtcgcagccc tgctgcgcgg cgtcaaagcg caccatgccg tcctggtcgg cctgcacctg
     6061 catttgcagc acggccgtgg cgtgcgcctc gtcgggccac gcgcgcaccg attcgagcag
     6121 cgcgcgcgag gcggcgttga gcgcgtaggc acgcacatcg gccacctgcg gtgcgcccgc
     6181 tggtggcgtg accagggcca cgcgcaggcg ctcgcgcgcc agcaaaagag ccagggtgcg
     6241 gcccaccacg cccgcaccac ggatacatac gtcaaaagtc tgcgccatgt gcgaattgta
     6301 gggatgcgcc cgccgcccct ggcgccgcga tggtcggccc ggccgtggcg gcggcgaaag
     6361 cccttggcgc gcgggatttg tccgcgctct taaaatggcg ggtttgtctc gctaccttac
     6421 gaaagccctg ccatgagcct gcaatgcggc atcgtgggcc tgcccaatgt gggcaagtcc
     6481 accctcttca acgccctcac caaggccggc atcgccgccg agaactaccc cttctgcacc
     6541 atagagccga acaccggcgt ggtcgaggtg cccgatccgc gcctggccca gctggccggc
     6601 atcatcacgc ccgagcgcgt ggtgccggcc atcgtcgagt ttgtggacat cgccggcctg
     6661 gtggccggcg ccagcaaggg cgagggcctg ggcaaccagt tcctggccca tatccgcgag
     6721 accgacgcca tcgtcaacgt ggtgcgctgc tttgaagacc cgaacgtgat ccacgtggcg
     6781 ggcaaggtcg atcccatctc cgacatcgag gtcatccaga ccgaactctg tctggccgac
     6841 ctggccacgg tggagaaggc tttgaaccgc tacagcaagg ccgccaagag cggcaacgac
     6901 aaggaggcgg ccaagctggt gtcgttgctg acgcccatcc aggccgcgct caacgagggc
     6961 aagccggcgc gcgccatagc ggtcagcaag gaagacgcgc cgctcttgaa gcagttctgc
     7021 ctgatcaccg ccaagccggc gatgtttgtc ggcaacgtgg ccgaggacgg cttcgagaac
     7081 aacccgctgc tggacaagct gaaggaatac gcggccgcgc agaacgcgcc cgtggtcgcc
     7141 atttgcgcaa aaatagaggc cgaaatggcc gagatgagcg acgaagaccg cgacatgttc
     7201 ctcgccgaga tgggccagga cgagcccggc ctgaaccgcc tgatccgcgc cggcttcaag
     7261 ctcttgggcc tgcagaccta tttcaccgcc ggcgtgaagg aggtgcgtgc ctggaccatc
     7321 cacgtcggcg acaccgcgcc ccaggcggcc ggcgtgatcc acggcgactt cgagcgcggc
     7381 ttcattcgcg cccagaccat cgccttcgac gacttcatcc agttcaaggg cgagcaaggc
     7441 gccaaggacg ccggcaagat gcgcgccgag ggcaaggaat atgtcgtcaa ggatggcgat
     7501 gtgatgaact tcctgttcaa cgtct
//
LOCUS       DAOMTQ010000139         7464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2253_length_7464_cov_26.467539, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000139 DAOMTQ010000000
VERSION     DAOMTQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_7464_cov_26.467539"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK518_12240"
     CDS             complement(<1..280)
                     /locus_tag="PK518_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49729.1"
                     /translation="MTLTELKYIVAVAREKHFGRAAEACYVSQPTLSVAVKKLEDELE
                     VKLFERSAGDVSVTPLGEEIVRQAQSVLEQAAEIKEIAKRGKDPLAGAL"
     gene            complement(355..2445)
                     /gene="recG"
                     /locus_tag="PK518_12245"
     CDS             complement(355..2445)
                     /gene="recG"
                     /locus_tag="PK518_12245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRM49730.1"
                     /translation="MVDQSPAQKALVKLGLTRPIDLALHLPLRYEDETRITPLRNTRE
                     GDMVQIEATVTASEVQLHPRRQLVVTVEDGTGSCELRFFSFYPSHQKTMAVGARLRVR
                     GEVRGGFWGRQMLHPAFRKAEGELPQALTPIYPTVAQLPQAYLRRAVASAMARVELPE
                     TLPPGLQPPVARFDRENGLQRPWNLRESLLFLHHPSPDVALATLQDHSHPAWQRLKAE
                     ELLAQQLSQLTAKRERALLRAPELRPAQAAGAPALHERLLAALPFALTAAQRRVGEEI
                     AHDLARPVPMHRLLQGDVGSGKTVVAALAACVCMDAGWQCALMAPTEILAEQHFTKLI
                     GWLEPLLAERGQQVAWLVGGQKKKERAAMLERIASGAAALVVGTHAVIQEQVQFKNLA
                     LAVIDEQHRFGVAQRLALRQKLARHGLEPHLLMMSATPIPRTLAMSYYADLDVSTIDE
                     LPPGRTPVVTKLIADSRKDQVVERIAAQVAAGRQVYWVCPLIEESEALDLSNATATHV
                     ALSEALPGVTVGLLHSRMPSAEKKAVMEAFKSGTMGVLVSTTVIEVGVDVPNASLMVI
                     EHAERFGLSQLHQLRGRVGRGAAASACVLLYSTGDSGRLGETAKGRLKAMAETTDGFE
                     IARRDLEIRGPGEFLGARQSGDALLRFADLATDGALLDWAREAAPQMLDQHPLLAEQH
                     IQRWLGTKSDYLKA"
     gene            2540..3780
                     /locus_tag="PK518_12250"
                     /pseudo
     CDS             2540..3780
                     /locus_tag="PK518_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
     gene            complement(3960..5135)
                     /locus_tag="PK518_12255"
     CDS             complement(3960..5135)
                     /locus_tag="PK518_12255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM49731.1"
                     /translation="MDTSFIWDDGFVTGLDVVDAQHHALVDLFNELNQALFSPAGDRQ
                     ALLADIYARLLAYAEYHFREEEQLMAQSGLDARHVQAHCRLHQQFVQQIRVMWARRGT
                     QADLGAMLVDFLTAWLGLHILGIDRSMARQIASRRQGLDAAEAFERERQRARHDDGMQ
                     TLLKMINRLYSALSVQNTQLASANQDLEERVTQRTRELELANERLRLLSRTDALLDIA
                     NRAHFNEWLEQACTLARRTQRPLGLVMIDVDCFKRYNDRYGHQQGDTCLQAVARAVAG
                     CLRRDADLVARYGGEELVVVLPDTGMAGATALAQRMVQAVQALALPHADSPVGPCVTI
                     SAGVCSQVPHAGADGASGSAALIAHADAALYQAKHQGRNRCVAHAEGGDAAGMLSKI"
     gene            5299..6399
                     /gene="queA"
                     /locus_tag="PK518_12260"
     CDS             5299..6399
                     /gene="queA"
                     /locus_tag="PK518_12260"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRM49732.1"
                     /translation="MPPSPRIHTLSDFDFELPELLIAQHPAPERSSSRLLDGRQSPPT
                     DRIFRELPGLLQPGDLVVFNDTKVVKARVFGEKASGGQLELLIERVLTGNEVVAHMKV
                     SKKPLPGATIHMAGGRRAGGFDATLLARWPDENGPLFHFALHGPAGESAWELMERHGH
                     LPLPPYIERQQNAAHDPDAAEDAERYQTVFARAPGAVAAPTAALHFDAAVLAQLQQRG
                     VETANVTLHVGAGTFQPVKTENLAEHQMHSEWYEIPLATLAALERCRQRGGRVLAVGT
                     TTVRTLESWAQSGQARGDTRIFITPGFAFQVVDLLLTNFHLPKSTLMMLVSAFAGYEH
                     IMLLYRHAIAQRYRFFSYGDAMLLQRRERTTP"
     gene            6620..>7464
                     /locus_tag="PK518_12265"
     CDS             6620..>7464
                     /locus_tag="PK518_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49733.1"
                     /translation="MAAQPSTRSAQQQLQRDERAAVLRRRRHAGLGNAQARQLPTVGL
                     ALSGGGVRSATFGLGLLRGLAAQGLFRRVDYLSTISGGGYVGAMLGRLISALGLAQAQ
                     QMLAAGHSDMLGWLRSNGRYLTPAGSRDVGILIVTYLRAFLAVHIEAFFALVPVALAL
                     TLPHLLQHSLGWLQTTAWQGWASPWFALALLWAAALLPGLLVGYWAAPETRSPDPAGR
                     RLYPGDAFLLAGLILVALALTLRPGRLPLLQAWRQAELVLPLLVLALWSAALGHASAM
                     ARLALA"
BASE COUNT         1142 a         2675 c         2473 g         1174 t
ORIGIN      
        1 tcagcgcgcc ggccagcggg tccttgccgc gcttggcgat ctccttgatc tcggccgcct
       61 gctccagcac gctttgcgcc tggcgcacga tctcctcgcc cagcggcgtg accgagacat
      121 cgcccgcgct gcgctcgaac agcttgacct ccagctcgtc ctcgagcttt ttcaccgcca
      181 ccgacagcgt gggctgcgag acatagcagg cctcggccgc gcggccaaaa tgcttttcac
      241 gggcgacggc gacgatgtat ttgagttcgg taagagtcat ctcgtggcaa tagcgaaaga
      301 ctaaaacgcg acattgtgca atgcgcggcc gcggcgcggt tgccgcgcgc cggctcacgc
      361 cttcagatag tcggactttg tgcccagcca gcgctgtatg tgctgctcgg ccagcagcgg
      421 gtgctggtcc agcatctgcg gcgcggcctc gcgcgcccag tccagcagtg cgccgtccgt
      481 ggccaggtcg gcaaagcgca acagcgcatc gcccgactgg cgcgcgccca aaaattcacc
      541 cgggccgcgt atctccaggt cgcgccgcgc gatctcgaag ccgtctgtcg tctccgccat
      601 ggctttcagg cggcccttcg ccgtctcgcc caggcggccg ctgtcgcccg tggagtacag
      661 cagcacgcag gccgaggccg cagccccccg ccccacgcgc ccgcgcagct ggtgcagctg
      721 cgacaggccg aagcgctcgg catgctcgat caccatcagg ctggcgttgg gcacgtccac
      781 gccgacctcg atgacggtgg tggagaccag cacgcccatg gtgccgctct tgaatgcctc
      841 catcaccgcc tttttctcgg cggatggcat gcgcgaatgc agcaggccca cggtgacgcc
      901 aggaagggcc tcgctcaacg ccacatgggt ggccgtggcg ttggacagat ccagcgcctc
      961 gctctcctcg atcaaggggc agacccagta cacctgccgc cccgccgcca cctgcgcggc
     1021 gatgcgctcg accacctgat ccttgcggct gtcggcgatc agcttggtga ccactggcgt
     1081 gcgcccgggc ggcagctcgt ctatggtgga gacgtccaga tccgcgtagt agctcatggc
     1141 cagggtgcgc ggtatgggcg tggcactcat catcagcagg tgcggctcca agccatggcg
     1201 agccaacttc tgccgcagcg ccaggcgctg ggccacacca aagcggtgct gctcgtcgat
     1261 caccgccagg gccaggttct tgaactgcac ctgctcctgg atgaccgcat gcgtgcccac
     1321 caccagggcc gccgcgccgc tggcgatgcg ctccagcatg gcggcgcgct ccttcttctt
     1381 ctgcccgcca accagccagg ccacctgctg gccgcgctcg gccaggagcg gctccagcca
     1441 gccaatgagc ttggtgaaat gctgctcggc caggatctcg gtgggcgcca tcagcgcaca
     1501 ttgccagccg gcatccatgc agacgcaggc ggcgagcgcc gcgaccacgg tcttgcccga
     1561 gcccacatcg ccctgcagca ggcggtgcat gggcacgggc ctggccaggt catgcgcgat
     1621 ttcttcgccc acgcggcgct gggccgcggt cagcgcaaag ggcagcgctg ccagcaggcg
     1681 ctcgtgcagg gccggtgcgc ccgccgcctg cgccgggcgc agctcgggcg cgcgcagcaa
     1741 ggcgcgctcg cgcttggccg tcagttgcga cagctgctgc gccagcagct cctcggcctt
     1801 caggcgctgc caggccgggt ggctgtgatc ctgcaacgtg gccagcgcca cgtcgggtga
     1861 tgggtgatgc aaaaataata gcgattcgcg cagattccac ggtctttgta gcccgttttc
     1921 cctatcaaac cgcgccacgg gcggctgcag tcctggcggc agggtctcgg gcagctccac
     1981 gcgcgccatg gcgctcgcca cggcgcggcg cagataggcc tggggcagct gggccacggt
     2041 gggatagatg ggggtcaatg cctggggcag ctcgccttcg gccttgcgaa aggccgggtg
     2101 cagcatctgc cggccccaga agccgcccct gacctcgccg cgcacgcgca gccgcgcacc
     2161 cacggccatg gtcttctggt gcgatgggta gaagctgaag aagcgcagct cgcagctgcc
     2221 ggtgccatcc tccaccgtca ccaccagctg gcggcgcggg tgcagctgca cctcgctggc
     2281 ggtcaccgtg gcctcgatct gcaccatgtc gccctcgcgt gtgttccgca aaggggtgat
     2341 gcgcgtttcg tcctcgtagc gcagcggcag gtgcagggcc aggtcgatgg ggcgcgtcag
     2401 gcccagcttg accagggcct tttgcgcggg gctttgatca accattgtca aagccgcgcc
     2461 ggcgtccggg caaggggttt cagcgtggtt ttgaaacaat taaaatagga agttactttc
     2521 gtcatcctga tcagttttca tgctcaaacg catccatgtg cagcagctca gcctgggaat
     2581 gtacctgcac gaactttgcg gctcgtggat ggagcatccg ttctggcgca cgcgttttct
     2641 gctcgaagac ccgcaggatc tgctgcgcat acgcgaaacc agcatccagg aggtgttgat
     2701 cgacatctcc aagggactgg atctggccaa cgcagccaca gcacaggccg aaggcgaggt
     2761 gagtgaagcc ccccaggcgt cgccggtgca ggcagagccg gtacaggcag agccgccgtc
     2821 ggccgccaca cccatggcgc aggagctgca gcgcgccgcc agcatttgcg ccaacgccaa
     2881 gcaggccgtg atcagcatgt tcagcgaggc gcgcatgggc cgtgcggtgg acgccagcag
     2941 cgccccgggc ctggtcagcg agatagcgga ctcggtgacg cgcaacccca gcgccctgat
     3001 cagcctggcg cgcatcaaga ccgccgacga atacaccttc atgcactcgg tggccgtgtg
     3061 cgcgctgatg gtgggcctgg cgcggcagat gggcctgaac gacgaggcca cgcagcaggc
     3121 gggcctggcc ggcctgctgc acgaccttgg caaggcccat gttccgctgg cgatcctgaa
     3181 caagccgggc aagctcagcg acgccgagtt cgccctgatg cgcggccacc ccggcgccgg
     3241 ccacgccctg ctgcagggcc agggtctgcc cgcgccggtg ctggacgcct gcctgcacca
     3301 ccacgagaag atcgacggca cgggctatcc gcaccgcctg ccggccgagc agatcaccac
     3361 cctggcgcgc atggcggcga tctgcgatgt gtacgacgcc atcacctcgg accgccccta
     3421 caaggccggc tgggacccgg ccgaatcgct gcgccgcatg gccgagtgga cgcgcgacca
     3481 tctggaccag cgcctgtacc aggccttcgt gaaaagcctg ggcatctacc ccgtgggctc
     3541 gctggtgcgc ctgacatcgg ggcgcctggg cgtggtcacc gagcagtcgc ccggcacgct
     3601 gctcacgccg cgcgtgaagg tttctattcc accaagtcgg acatgcgcat accgcccgag
     3661 ctgatcgacc tgtccgcgcc cggctgccgc gagcagatcg ccgggcgcga agacccggcg
     3721 cgctggcagt tcccggatct gaacgaactg tggtccgggc tgccgcacca gccctggtga
     3781 gcgccggcga cggcaaaaaa accaccgccg ttctgatcca gccatagcgg cctgcgaggg
     3841 gcaagctatg gcatcaggag cgcgccgcgc tcgccactgc aacgatttct atcgaatgcc
     3901 cgagaagtag cggttcaggc aatatttcat taaaaccgcc acgcagcaag cgcgaatagc
     3961 tatattttcg agagcattcc agcggcatcc ccaccctccg catgcgccac gcaacggttg
     4021 cgcccctggt gcttggcctg gtagagcgcg gcgtcggcgt gggcgatcaa cgccgccgag
     4081 ccgctggcgc cgtccgcacc cgcatggggc acctggctgc acacgccggc gctgatggtc
     4141 acgcagggcc cgaccggcga gtcggcatgc ggcagggcca gcgcctgcac ggcctgcacc
     4201 atgcgctgcg ccagggccgt ggcgccggcc atgccggtat cgggcaacac caccaccagc
     4261 tcctcgccgc cgtagcgcgc caccaggtcg gcgtcgcggc gcaggcagcc ggccacggcg
     4321 cgcgccacgg cctgcaggca ggtgtcgccc tgctggtggc cgtagcggtc gttgtagcgc
     4381 ttgaaacagt ccacgtcgat catcaccagg cccagtggcc gctgggtgcg gcgcgccagg
     4441 gtgcaggcct gctccagcca ttcgttgaaa tgcgcgcggt tggcgatgtc cagcagcgca
     4501 tcggtgcgcg agagcaggcg caggcgctcg ttggccagct ccagctcgcg cgtgcgctgg
     4561 gtcacgcgct cctccagatc ctggttggcg ctggccagct gggtgttctg caccgacagc
     4621 gccgaataca gccggttgat catcttcagc agcgtctgca tgccgtcgtc atgccttgcg
     4681 cgctggcgct cgcgctcgaa ggcctcggcc gcgtccagcc cctggcgccg ggacgcgatc
     4741 tgccgcgcca tcgaccggtc tatgcccagg atatgcagac ccagccacgc ggtcagaaag
     4801 tcgaccagca tggcgcccaa atcggcctgc gtgccgcgcc gcgcccacat cacgcgtatc
     4861 tgctgcacga actgctggtg cagccggcaa tgcgcctgga catggcgcgc gtccaggccg
     4921 ctttgcgcca tcagctgctc ctcttcgcgg aagtggtact cggcataggc cagcaggcgg
     4981 gcgtagatat cggccagcag cgcctggcgg tcgccggccg gcgagaacag cgcctggttc
     5041 agctcgttga acagatccac caacgcatgg tgctgcgcat ccaccacatc caggccggtg
     5101 acaaagccat catcccaaat gaaagaggta tccatacgcc gtcgaacgat tgcttgacaa
     5161 aagacaaagt ctatgggatt tggagtgcga tagcttgacc tttctaaagt gcccacgccg
     5221 ccacggggcc gcgccgcagt tgcgacaatc gccccctgcg cctgccgcca tggccggccg
     5281 cctttgtcaa gaatccgcat gcctcccagc ccccgcatcc ataccctcag cgacttcgac
     5341 ttcgagctgc ccgagctgct catcgcccag caccccgccc ccgagaggag cagctcgcgc
     5401 ctgctcgacg ggcgccagag tccgcccacc gaccgcatct tccgcgagct gcccggtctg
     5461 ctgcagccgg gcgacctggt ggtgttcaac gacaccaagg tggtcaaggc gcgcgtcttt
     5521 ggcgagaagg ccagcggcgg ccagctggag ctcttgatcg agcgcgtgct gacgggcaac
     5581 gaagtcgtgg cgcacatgaa ggtcagcaaa aagcccctgc ccggcgccac catccacatg
     5641 gccggcgggc gccgcgcggg cggcttcgac gccaccctgc tcgcgcgctg gcccgacgag
     5701 aacggcccgc tgtttcactt cgccctgcat ggcccggccg gcgagagcgc ctgggagctg
     5761 atggagcgcc acggccacct gccgctgccg ccctacatag agcgccagca aaacgccgcc
     5821 cacgacccgg acgcggccga ggacgccgag cgctaccaga ccgtgttcgc ccgcgccccc
     5881 ggcgccgtgg ccgcgcccac ggcggcgctg catttcgacg ccgccgtgct ggcgcagctg
     5941 cagcagcgcg gcgtggagac cgccaacgtc accctgcatg tgggcgccgg caccttccag
     6001 ccggtgaaga ccgagaacct ggccgaacat cagatgcaca gcgagtggta tgagatcccg
     6061 ctggccacgc tggccgccct ggagcgctgc cgccagcgcg gcgggcgggt gctggccgtg
     6121 ggcacgacca ccgtgcgcac gctcgagtcc tgggcgcaga gcggccaggc ccggggcgac
     6181 acgcgcatct tcatcacgcc gggctttgca ttccaggtgg tggacctgct gctgaccaac
     6241 ttccacctgc ccaagagcac gctgatgatg ctggtcagcg ccttcgccgg ctacgagcac
     6301 atcatgctgc tgtaccgcca cgccatcgcc cagcgctacc gtttcttcag ctacggcgac
     6361 gccatgctgc tgcagcgtag ggagaggaca accccctgag cggcttcgcc gcttccccct
     6421 tctctcgaat ggcggcgcca ttcgggaagg gggacgacgc cagcgcggcg gggcggccct
     6481 tgcgcggcgt ctgctggcct ggggtgtgcc agtttcatgc gccgcgcccc ttcttcaccc
     6541 caaaaaagcg gttgctcact acgccggcgc attcggccgg ctgttgacgt ggccctggcg
     6601 cgcgcctacc gtatgcgcca tggccgccca accgtccacc cgctcggcgc agcagcaatt
     6661 gcagcgcgat gaacgcgcgg cggttctgcg ccggcgccgc catgccggcc tgggcaacgc
     6721 ccaggcgcgc cagctgccca cggtggggct ggcgctgtcg ggcggcggcg tgcgcagcgc
     6781 cacctttggc ctgggcctgt tgcgtggcct cgcggcgcag gggctgttca ggcgcgtgga
     6841 ctacctgtcc accatctccg gcggcggcta cgtgggcgcc atgctggggc gactgatcag
     6901 cgcgctgggc ctggcgcagg cgcagcagat gctggccgcg ggccactccg acatgctcgg
     6961 ctggctgcgc agcaacggcc gctacctcac gcccgccggc tcgcgcgacg tgggcattct
     7021 catcgtcacc tatctgcgcg cctttctggc ggtgcatatc gaggccttct tcgcgctggt
     7081 gccggtggcg ctggcgctga ccctgccgca tctgctgcag cacagcctgg gctggctgca
     7141 gaccacggcc tggcagggct gggcctcgcc ctggtttgcc ctggctctgc tgtgggccgc
     7201 ggccctgctg cccggcctgc tggtgggcta ctgggcggcg cccgagacgc gcagcccgga
     7261 tcccgccggg cgccgcctct accccggcga cgcttttttg ctggcggggc tgatcctggt
     7321 ggccctggcg ctcacgctgc gcccgggccg gctgccactg ctgcaggcct ggcgccaggc
     7381 cgagctggtg ctgcccctgc tggtgctggc gctgtggtcg gccgcgctgg gccatgccag
     7441 cgccatggcc cgcctggcgc tggc
//
LOCUS       DAOMTQ010000140         7371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2294_length_7371_cov_25.069847, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000140 DAOMTQ010000000
VERSION     DAOMTQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2294_length_7371_cov_25.069847"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK518_12270"
     CDS             <1..354
                     /locus_tag="PK518_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HRM49734.1"
                     /translation="GRSNIVGKPMAMMLLAANATVTVCHSGTADLAAMTRQADIVVAA
                     VGKRNVLTADMVKPGAVVIDVGMNRNDAGKLCGDVDFDGVKQVAGWITPVPGGVGPMT
                     ITMLLVNTLEAAERL"
     gene            405..2435
                     /locus_tag="PK518_12275"
     CDS             405..2435
                     /locus_tag="PK518_12275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRM49735.1"
                     /translation="MSNPLLDFSAQIPFDRITPADVAPAVDQLLARADAALASVTAPD
                     FPAQWGSIARVLDVATEELGRAWGAVSHLNSVADTPELRAAYNAALPRVTEFWTRLGA
                     DERLYAKYKAIDPATLNAEQRQARSNAMRNFVLSGAELQGAAKERFAAIQERQAELQQ
                     KFSENALDATDAYAYYASAEEIEGLPADVALTARAAAEDEGKPGYKLTLKMPCYLPVM
                     QFAKSSALRERLYHGYVTRASDQAEGDAKRFDNSAVMTEILALRREEAQLLGYENFGQ
                     VSLVPKMAHTPEQVTHFLRDLAQRARPFAEKDVADLRAFAAEQLGLQDPQAWDWPYIS
                     EQLKEARYAFSEQELKQYFTAPKVLAGLFKIVETLFEVAIRRDQAPVWNEAVEFYRIE
                     RDGKLVGQFYLDQPARAGKRGGAWMDDARTRWLRPDTGRLQTPVAYLVCNFAQGVDGK
                     PPLLTHDDVITLFHEFGHGLHHMLTHVNERDVSGISGVEWDAVELPSQFMENFCWEWE
                     VLKHLTAHVDSGAPLPRALYDKMIAAKNYQAGMQTLRQIEFALFDMLLHTEHEPQGDL
                     LALLARVRAEVAVLTPPAFSRTAHTFSHIFAGGYAAGYYSYKWAEVLSADAYAAFEET
                     TGADGVPSLETGRRYRQAILEAGGSRPAMESFKAFRGREPTLDALLRHQGMA"
     gene            2553..3191
                     /locus_tag="PK518_12280"
     CDS             2553..3191
                     /locus_tag="PK518_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRM49736.1"
                     /translation="MTILRFVATCATLVALATAAQAQQVYRIVGPDGKVTFSDRAPSG
                     GAEAAQAGSGQRGGNANDALPYQLRQVAARFPVTLYSGNDCQPCNSARNLLINRGVPF
                     TERTITSNEDIDALKQLSGVASLPFGTIGGQQLKGFSDSEWSQYLDAAGYPAQSQLPP
                     SYRRPAPTPLVAAKPAAPATETAAPAAATPAARPAPAPATGKSPSNPAGIIF"
     gene            complement(3198..3887)
                     /gene="rpiA"
                     /locus_tag="PK518_12285"
     CDS             complement(3198..3887)
                     /gene="rpiA"
                     /locus_tag="PK518_12285"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRM49737.1"
                     /translation="MTAPTTLSQDELKTLVGQAALHYVVPGEIVGVGTGSTVNKFIDA
                     LASMKDSIRGAVSSSEASTARLQALGIPVFDANEVERLSVYIDGADEIDGAGYMVKGG
                     GAALTREKIVAALAERFVCIADESKLVPALGAFPLPVEVIPMAAAQIARRFAAMGGQA
                     TLRCKDGQPLVTDNGQHILDVRGLSIAEPLAFESQVNQWPGVVTVGVFAHQKAHVCLL
                     GTAQGVRTLQF"
     gene            3923..4969
                     /locus_tag="PK518_12290"
     CDS             3923..4969
                     /locus_tag="PK518_12290"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRM49738.1"
                     /translation="MSLLPYALARPFLFGMDAEAAHELTMDMLARGQRTPLQWAWCND
                     TVSDPVTLAGLRFPNRVGLAAGLDKNARCIDGLAAMGFGFVEVGTVTPKGQPGNPKPR
                     MFRLPQARALINRLGFNNEGLDAFIANVQQSQVRRQGKPLLLGLNIGKNASTPIEDAT
                     RDYLTCLEGVYPHADYVTVNISSPNTQNLRALQSDEALDGLLGAVAGHREKLAAAQGR
                     RVPIFVKIAPDLSEEQVSVIAATLQRHGMDGVIATNTTISRDAVQGLPHAAETGGLSG
                     APVLAASNAVIAQLRAALGPAFPIIGVGGIMSAEDAVSKIRAGADLVQIYTGLIYEGP
                     TLIGRAARAIQAMR"
     gene            complement(5033..5332)
                     /locus_tag="PK518_12295"
     CDS             complement(5033..5332)
                     /locus_tag="PK518_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49739.1"
                     /translation="MPGTTPTPTPAQQQVLDRIHAQRERLVKRRQAMRQARAAAATQG
                     RVDPNDPLLTRLLTFARLHPLALGAVAGIAALAGPNRVLRWVGVVLPAIARMRRG"
     gene            complement(5332..5736)
                     /locus_tag="PK518_12300"
     CDS             complement(5332..5736)
                     /locus_tag="PK518_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRM49740.1"
                     /translation="MARVNWAGLLNLQGLAGRWRSAMGEGATAVEDRMELARLEWADH
                     KRRLALLALLLVVFGVLAVVALLLLSAAVLVQFWDTSQRALAAWLLAGLWLVACGAVL
                     AVLLSMARKTRSAFALTRRELAQDWRVIKEQL"
     gene            complement(5744..6052)
                     /locus_tag="PK518_12305"
     CDS             complement(5744..6052)
                     /locus_tag="PK518_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HRM49741.1"
                     /translation="MSSVSETVSTSQGELEKLVGDLRGLLASRDLDGIPEIKQLRQRL
                     DDGIQTVRDATVRAAQQAADQARDAARAADRYAHDEPWRVASAALAVGALVGFLLARR
                     "
     gene            complement(6125..7225)
                     /locus_tag="PK518_12310"
     CDS             complement(6125..7225)
                     /locus_tag="PK518_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM49742.1"
                     /translation="MSDLVDCIVVGAGVVGLAVARALALDGREVLVLEAANAIGTETS
                     SRNSEVIHAGIYYPQGSLKARLCVQGKTMLYDYCAERGVPYQRSGKLIVATAPGQLAQ
                     LDGIRQRAAANGVDDLRWLTREEARALEPALECVAALLSPSTGIVDSHALMLALLGDL
                     ENAGGLLALNSPLAQAQCAQDAIHIEAKDGTRISARTVVNAAGLHAPGLARCFAGLDP
                     ALVPQAAYAKGNYFTLSGRAPFSHLIYPVPEAAGLGVHLTLDLGGQAKFGPDVQWVDG
                     PDDLVVDASRGQAFYAEVRKYWPGLPDGALAAGYAGIRPKIHGPQEPAADFLIQGPRQ
                     HGVAGLVNLFGIESPGLTSALAIGAHVADILR"
BASE COUNT         1325 a         2631 c         2338 g         1077 t
ORIGIN      
        1 ggccgcagca acatcgtcgg caagcccatg gccatgatgc tgctggccgc caacgccacc
       61 gtcaccgtct gccacagcgg cacggcagat cttgccgcca tgacgcgcca ggccgacatc
      121 gtggtcgccg ccgtgggcaa gcgcaatgtg ctcacggccg acatggtcaa gcccggcgcc
      181 gtggtcatcg acgtgggcat gaaccgcaac gacgcgggca aactctgcgg cgacgtggat
      241 tttgacggcg tcaagcaggt cgcgggctgg atcacccccg ttcccggcgg cgtcggcccc
      301 atgaccatca ccatgttgct ggtcaacacc cttgaagcgg ccgagcgcct gtagttgatc
      361 gatcgctgct tgaacccgct cccactgccg ccatctaaac ccgcatgagc aatcccctcc
      421 tcgacttctc ggcccagatc ccgttcgacc gcatcacccc cgccgacgtg gcgcccgccg
      481 tggatcagct gctggcacgc gccgacgcgg ccttggccag cgtcaccgcg cccgactttc
      541 ccgcgcagtg gggctcaatc gcccgcgtgc tggacgtggc caccgaggag ctgggccgcg
      601 cctggggtgc ggtgagccac ttgaacagcg tggccgacac gcccgagctg cgtgccgcct
      661 acaacgccgc cctgccgcgc gtgaccgagt tctggacgcg cctgggcgcc gacgagcgcc
      721 tgtacgccaa gtacaaggcc atagacccgg cgacgcttaa cgccgagcag cgccaggcgc
      781 gcagcaacgc catgcgcaac ttcgtgctct cgggcgccga gctgcaaggc gcggccaagg
      841 agcgcttcgc cgccatccag gagcgccagg ccgagctgca gcaaaaattc agcgaaaacg
      901 cgctggacgc caccgacgcc tacgcctact acgccagcgc cgaggagatc gaaggcctgc
      961 ccgccgacgt ggccctgacc gcgcgcgccg cggctgaaga cgagggcaag ccgggctaca
     1021 agctcacgct gaagatgccc tgctacctgc cggtgatgca gttcgccaag agcagtgcgc
     1081 tgcgcgagcg gctgtatcac ggctatgtca cgcgcgcctc ggatcaggcc gagggcgatg
     1141 cgaaacgctt tgacaacagc gccgtcatga ccgagatcct ggcgctgcgc agggaagagg
     1201 cgcagctact gggctacgag aatttcggcc aggtatcgct ggtgcccaag atggcgcaca
     1261 cgccggagca ggtcacccac ttcctgcgcg acctggccca gcgcgcccgg ccctttgccg
     1321 agaaagacgt ggccgacctg cgcgcctttg cggccgagca gctgggcctg caggatccgc
     1381 aggcctggga ctggccctat atctccgagc agctcaagga agcgcgctat gccttcagcg
     1441 agcaagagct gaaacaatat ttcacggcgc ccaaggtgct ggccggcctg ttcaagatcg
     1501 tcgagaccct gttcgaggtg gcgatacgcc gcgaccaggc gccggtgtgg aatgaggccg
     1561 tggagttcta ccggatcgag cgtgacggca agctggtggg ccagttctac ctggaccagc
     1621 ccgcgcgtgc cggcaagcgc ggtggcgcct ggatggacga cgcgcgcacg cgctggctgc
     1681 gccccgacac cggccggctg cagacgcccg tggcctatct ggtgtgcaac ttcgcgcaag
     1741 gagttgacgg caagccgccc ctgttgaccc acgacgacgt gatcaccctg ttccacgagt
     1801 tcggccatgg cctgcaccac atgctgaccc atgtgaacga gcgcgatgtc tcgggcatca
     1861 gcggcgtgga gtgggatgcg gtggagctgc ccagccagtt catggaaaac ttctgctggg
     1921 aatgggaggt gctcaagcac ctgacggccc atgtggacag cggcgcgccg ctgccgcgcg
     1981 ccctctacga caagatgatc gccgccaaga actaccaggc cggcatgcag accctgcgcc
     2041 agatcgagtt tgcgctgttc gacatgctgc tgcacaccga gcatgagccg cagggcgacc
     2101 tgctggcatt gctggcgcgc gtgcgcgccg aggtggcggt gctgacgccg ccggccttca
     2161 gccgcacggc ccacaccttc agccatatct tcgccggcgg ctacgcggcc ggctactaca
     2221 gctacaaatg ggccgaggtg ctgagcgccg acgcctacgc cgccttcgag gaaaccaccg
     2281 gcgccgacgg cgtgcccagc ctggagaccg gccggcgcta ccgccaggcc attctggagg
     2341 caggcggcag ccgcccggcc atggaatcct tcaaggcctt ccgcggccgg gagcccacgc
     2401 tggacgcctt gctgcgccac cagggcatgg cctgataaaa agtgagctgc ttgcgcttgt
     2461 ctaccaaggt atttaaacta ttttcagcgt taaaccagcc agatatcagc gcaagcagct
     2521 attgtttttg agaaccgcac aggattcgcc gcatgaccat cctccgcttt gtcgccacct
     2581 gcgccaccct cgtcgccctg gccacggccg cgcaggccca gcaggtctac cgcatcgtcg
     2641 ggcccgacgg caaggtcacg ttctccgacc gcgcacccag tggcggcgcc gaagcggcac
     2701 aggcgggcag cggccagcgc ggcggcaatg ccaatgacgc cctgccctat cagctgcgcc
     2761 aggtggcggc gcgctttccc gtcacgctct actccggcaa cgactgccag ccctgcaaca
     2821 gcgcgcgcaa cctgctcatc aaccgtggcg tgcccttcac cgagcgcacc atcaccagca
     2881 acgaagacat agacgcgctc aagcaattga gcggcgtggc cagcctgccg ttcggcacca
     2941 taggcggcca gcagctcaag ggtttttccg acagcgagtg gagccagtac ctggacgccg
     3001 ccggctaccc cgcgcagtcg cagctgccgc ccagctaccg ccgcccggcg ccgacgccgc
     3061 tggtggcagc caaaccggcg gcaccggcca cggaaaccgc agcacctgcc gccgcgacac
     3121 cggcggcacg cccggcgccg gcacccgcga caggcaagtc gccgtccaac ccggccggca
     3181 tcatctttta gaaccgttca gaactgcagc gtgcgcacgc cctgcgccgt gcccagcagg
     3241 cagacatggg ccttctggtg cgcgaaaacg cccacggtga ccacgccagg ccattggttg
     3301 acctggctct cgaaggccag cggctcggca atcgataggc cgcgcacgtc caggatatgc
     3361 tggccgttgt cggtcaccaa cggctggcca tccttgcagc gcagcgtggc ctggccgccc
     3421 atggcggcaa aacgccgcgc gatctgcgcc gccgccatcg ggatcacctc caccggcagc
     3481 gggaaggccc ccagcgccgg caccagcttg gactcgtcgg cgatgcagac aaagcgctcg
     3541 gccagtgcgg ccacaatctt ctcgcgcgtc agcgccgcgc cgccgccctt gaccatatag
     3601 ccggcaccgt cgatctcgtc cgcgccgtcg atgtagaccg acaggcgctc gacctcgttg
     3661 gcatcaaaca ccggtatgcc cagcgcctgc aggcgcgcgg tgctggcctc ggagctcgac
     3721 accgcgccgc gaatgctgtc cttcatgcta gccaacgcat caatgaactt gttcaccgtc
     3781 gagcccgtgc ccacgcccac gatctcgccg ggcaccacat agtgcagcgc ggcctggccg
     3841 accagggtct ttagttcgtc ttgggaaagc gtggtgggtg cagtcatggg agaaaatcgg
     3901 gagtgaacct tgaattatct ccatgtccct acttccctac gccctcgccc gcccttttct
     3961 gttcggcatg gatgccgaag ccgcccacga actgaccatg gacatgctgg cccgcggcca
     4021 gcgcacgccg ctgcaatggg cctggtgcaa cgacaccgtg agcgaccccg tcacgctagc
     4081 cggcctgcgt tttcccaacc gcgtgggtct ggccgccggc ctggacaaga acgcgcgctg
     4141 cattgacggc ctggccgcca tgggttttgg cttcgtcgag gtgggcaccg tcacgcccaa
     4201 gggccagccg ggcaacccca agccgcgcat gttccgcctg ccccaggcgc gcgccctgat
     4261 caaccgcctg ggcttcaaca acgaggggct ggacgccttc atcgccaacg tgcaacagtc
     4321 ccaggtgcgc cggcagggca agccgctgct gctgggcctg aacatcggca agaacgccag
     4381 cacgcccatc gaggacgcca cgcgcgacta cctgacctgc ctggagggcg tgtacccgca
     4441 tgccgactat gtgacggtga acatcagctc gcccaacacg cagaacctgc gcgcgctgca
     4501 aagcgacgag gcgctggacg gcctgcttgg cgccgtggcc gggcaccgtg aaaagctcgc
     4561 tgccgcccag ggccggcgcg tacccatctt tgtgaagatc gccccggact tgagcgagga
     4621 gcaagtgagc gtgattgccg ccacgctgca acgccacggc atggacggcg tgatcgccac
     4681 caacaccacc atcagccgcg acgcggtgca ggggctgccc catgccgcag aaaccggcgg
     4741 cctgagcggc gcaccggtgc tggcggcgag caacgccgtc atcgcgcagc tgcgcgccgc
     4801 gctggggccg gccttcccca tcatcggcgt gggcggcatc atgagcgccg aggacgcggt
     4861 gagcaaaatc cgcgcgggtg ccgacctcgt gcagatctac accggcctga tctacgaggg
     4921 cccgaccctg atcggccgcg ccgcacgcgc catccaggcc atgcgctgac caggccatgc
     4981 gctgaaatag caaagggccg cgtctgcagc ccttggattg aaacgccggc aatcagccgc
     5041 gccgcatgcg cgcaatggcc ggaagcacca cgcccaccca gcgcagcacg cgattcggcc
     5101 cggccaatgc ggcaatgccc gcaaccgcgc ccagcgccag agggtgcagc cgtgcaaacg
     5161 tcagcaagcg cgtcagcagg ggatcattgg gatccacccg cccctgcgtg gccgcagccg
     5221 cacgcgcctg gcgcatggcc tggcggcgct tgaccaggcg ctcgcgctgc gcatggatgc
     5281 ggtcgagcac ctgttgctgc gcgggggtcg gtgtgggggt tgtgccgggc atcacagctg
     5341 ctccttgatc acgcgccagt cctgcgccag ctcgcggcgc gtgagcgcaa acgcgctgcg
     5401 cgtcttgcgt gccatcgaca gcaagacggc caacaccgcg ccacaggcga ctaaccacag
     5461 gcccgccagc aaccaggccg ccagggcgcg ctgcgacgtg tcccagaact gcaccagcac
     5521 cgccgcggac agcagcaaca aggccaccac ggccagcacg ccaaagacga ccagcagcaa
     5581 cgccagcaga gccagacggc gtttgtggtc cgcccactcc agccgcgcca gctccatgcg
     5641 gtcttcgaca gcggttgcgc cctcgcccat ggccgagcgc cagcgcccgg ccaggccctg
     5701 caggttcagc aacccggccc agttgacacg cgccatcgac ggatcagcgg cgcgccagca
     5761 aaaaacccac caaggcccct acggccagcg cggcgctggc cacgcgccag ggttcgtcat
     5821 gggcgtaacg gtcggcggca cgggcggcgt ccctggcctg gtcggccgcc tgctgcgccg
     5881 cacgcaccgt ggcatcgcgc acggtctgga taccgtcgtc caggcgctgg cgcagttgct
     5941 tgatctcggg aatgccgtcc agatcgcggc tggccagcag gccgcgcagg tcgccgacca
     6001 gcttttcgag ttcaccctgg gatgtggaaa cggtttcgga tacggaggac atgaacaagc
     6061 ctttcatagc aggaaacaac gggaagacgc catacatggg aggcacgagg gcggatgcgt
     6121 catcttagcg cagtatgtcg gccacatgcg caccaatcgc caaggcgctg gtcaaacccg
     6181 gagattcgat gccaaacaga ttcaccagtc cggccacgcc atgctggcgc ggcccctgga
     6241 tcagaaaatc cgccgccggc tcctgcggac cgtggatctt cgggcgtatg ccggcatagc
     6301 cggctgccag cgcaccgtca ggcaggccag gccagtattt gcgcacctcg gcataaaagg
     6361 cctggccgcg gctggcgtcc accaccaggt catcggggcc atctacccat tgcacgtcgg
     6421 gcccgaactt ggcctggccg cccagatcca gcgtcaagtg cacgcccagg ccggcagcct
     6481 cgggcacggg gtagatcaaa tggctgaacg gcgcgcggcc cgacagcgtg aagtaattgc
     6541 ccttggcgta ggccgcctgc ggcaccaggg caggatcgag ccccgcaaag cagcgcgcca
     6601 gaccgggcgc atgcaggccg gccgcattca ccaccgtgcg ggcgctgata cgggtgccgt
     6661 ctttagcttc aatatgtata gcgtcttgcg cacactgcgc ctgcgccagg ggcgaattca
     6721 atgccaaaag accaccggca ttctccagat caccgagcag cgccagcatc agcgcatggc
     6781 tgtccacgat gccagtgctg ggcgacagca gcgcggccac gcattccagc gcgggctcca
     6841 gcgcacgcgc ctcttcgcgc gtgagccaac gcaggtcatc caccccgttg gctgcggcgc
     6901 gctgccggat gccgtcgagc tgcgccagtt gcccgggcgc ggtggccacg atgagcttgc
     6961 cgctgcgctg gtagggcacg ccgcgctcgg cgcaatagtc gtacagcatg gtcttgccct
     7021 gcacgcacag ccgcgccttg agcgagccct gggggtagta gatgccggcg tgtatcacct
     7081 cgctgttgcg cgagctggtt tcggttccga tggcgttcgc cgcctccagc accaagacct
     7141 cgcgcccatc cagggccagt gcccgtgcta ctgccagacc caccacgccg gcgcccacga
     7201 cgatgcaatc caccaagtca ctcatggtta tctccgttca aacaatgtta gacagtgcga
     7261 tcaggcaaca aaaaacccct tgcaggcaag ccttgcaagg ggttgtgttg gtgggcgata
     7321 catggatcga acatgtgacc cctgccgtgt gaaggcagtg ctctaccgct g
//
LOCUS       DAOMTQ010000141         7342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2303_length_7342_cov_20.121175, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000141 DAOMTQ010000000
VERSION     DAOMTQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_7342_cov_20.121175"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK518_12315"
     CDS             complement(<1..423)
                     /locus_tag="PK518_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRM49743.1"
                     /translation="MPILAWARTPVAPVGGALAACRPHELAAPLVARLLADAGLPASA
                     VDAVVLGNALGAGGNPARVLALAAGLPERTPAITLDSQCCAGLDAITHACGLLALGQA
                     QVVIAGGAEAWSRAPLRLQPVPLADAVAQARATLASAEA"
     gene            complement(429..1889)
                     /locus_tag="PK518_12320"
     CDS             complement(429..1889)
                     /locus_tag="PK518_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM49744.1"
                     /translation="MARQLRPGGPLPALFDWVHGPLAHWARTRPDALALANERQRLSF
                     AELHARVQARARRLDAEGAPATVLLGGDAGTLDGLVDFLGVIASGRCAAMGDAQWPAV
                     VAQAVAERLPVPAGAPGQGATPLSPFYIGFTSGSTGLPKGFMRHHRSWAESFRVSLRD
                     FGPAAATRVLAPGRMSHSLFLFGALLGLWTGAGALLQERFSAPRALATLQAGAAPLLV
                     AVPSQLVLMLQYAERRRLAPIAGVELVLISGARWLRERTPALRRLFPRARIVEFYGAS
                     EASYIAWMDADPAAPAQAVGRAFSNVDLHIGEHPEAPALPAGQPGLIWVRSPMLFMDY
                     VNQADATAALRVGDWLSVRDMGHLDASGLLHLCGRENRMLVTQGKNLFPEEVEQRLLA
                     HPAVAQASLQGAFDELRGSSVHAVLQWQGDAPAGAQQLAAWCREALEPFKTPRHWWLW
                     KGDWPQTASGKTDHGAIARALADTPVGASPLLEPWP"
     gene            complement(1919..2503)
                     /locus_tag="PK518_12325"
     CDS             complement(1919..2503)
                     /locus_tag="PK518_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRM49745.1"
                     /translation="MTTAVSRKASHSMALVALFAALMAVMGLIPKIDLPLGVPITLQS
                     LGVMLAGCLLGARLGFQAIALFLLAVAAGLPLLAGGRGGLGVFMAPSAGYLLGYALAA
                     GVTGALMRLLPQGQPLRTAISAFIASALGGLVFLHAMGIAGLVLLAKMPLEKAFFADL
                     VFVPGDLVKCLLCAVVVHTVAKAMPHWPFGGRER"
     gene            complement(2534..3127)
                     /locus_tag="PK518_12330"
     CDS             complement(2534..3127)
                     /locus_tag="PK518_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HRM49746.1"
                     /translation="MGSLYSEHLTWLHRWPAGAKLACMALLGTLLFLFNELWLLAAAG
                     LACALLWCSLGPATHKVRRLIRSVLVAAALVAGFHLWLGRPDVAAVSALRLACASTLG
                     VALTVTTRPTDLLLVLDALLAPLARLGLRPERLSLQLALMLRFTEHFFVQWQKLDEAH
                     RLRTGRPGGLRLIAPLTVQMLQAAQRVADALYARLGG"
     gene            complement(3137..3913)
                     /locus_tag="PK518_12335"
                     /pseudo
     CDS             complement(3137..3913)
                     /locus_tag="PK518_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4243..5448
                     /locus_tag="PK518_12340"
     CDS             4243..5448
                     /locus_tag="PK518_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49747.1"
                     /translation="MTTTSSTLERRNLGLLVAVQSLGGASPPIIISLGGLVGQMLSST
                     PSAATLPVSLYQLGLALSTLPAAWLIHRAGRRTAYLLGAVMGMLSGLVAAWGISQSDF
                     MLFCLGTTLAGFYGACVQSYRFAAIDAVGDPARHAGAISRVMVGGLIAAVIGPQVVIW
                     TRDSLPMTPFAGSFYSQAALALLALPLLLLLRLPPAPPKAVAHGARDLSEIVRTPQFI
                     VAATAGVVSYGLMAFLMTAAPMAMVGCGHSVGEAALGIQWHVLAMFGPSFFTGRLMDR
                     FGKRRITALGLILIAASGALALMGLALTHFWGALVLLGLGWNFGFIGATALLTEAYRP
                     SERAKVQALNDFLVFGTVAVASFGSGQLLHSVGWDGINLGMLPLVVVVLALLAWQGLR
                     QRQQMTARG"
     gene            5468..6076
                     /locus_tag="PK518_12345"
     CDS             5468..6076
                     /locus_tag="PK518_12345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM49748.1"
                     /translation="MQSASDEWFDEAYYQRYYFNKKTSVVDPAHTERLGAFVCSYLQY
                     LRVPVARVLDMGCGIGLWRDMVARHLPGAQYQGVEYSAYLCQRFGWQRGSVVDYPSAT
                     PYDFVICQGVLPYLGVQDLRRALANLGRLCQGALYLEAVTLEDYERGTIDEDLTDARQ
                     HRHRAALYRQGLARHFTDMGGGLWLSHRAQVPLFALEARDGA"
     gene            6073..6750
                     /locus_tag="PK518_12350"
     CDS             6073..6750
                     /locus_tag="PK518_12350"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HRM49749.1"
                     /translation="MTASPPRRSRCPRCLRPDATCLCPAVRSVASQVQVLILMHPQEE
                     HQAKGTARLLHLCLPASRLAVGETFAAQELARLLQEPWCDADARAPRHAMLLYPPTPS
                     DAQHPMPTPPPMPADWLSAPERLRLVLLDGTWRKSRKMLWSNPTLQALPRLALHHVPA
                     SRYAIRKAHAPHQLSTLEATQQALRHLEPANAGIAALGAAMQDFMARQQAYWPAAHWT
                     MLNATDS"
     gene            6766..>7342
                     /locus_tag="PK518_12355"
     CDS             6766..>7342
                     /locus_tag="PK518_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838418.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRM49750.1"
                     /translation="MTQRYPLPDLNTLPEDIRTRILEVQDKAGFIPNVFLAFARRPDE
                     WRAFFAYHDALMLREQGSLTKGEREMIVTATSAANQCLYCVVAHGAILRIYEKNPLVA
                     DQVAVNHRKADISARQQAMLDFAMKVCLRAQEIDDADFEALHPHGFDDEDIWDIAAIT
                     AFFGLSNRMANFSGMQPNPEFYLMGRMPREKK"
BASE COUNT         1161 a         2668 c         2515 g          998 t
ORIGIN      
        1 ggcctcggcc gaggccagtg tggcgcgcgc ctgggccaca gcatcggcca gcggcacggg
       61 ctgcaggcgc agcggcgcgc ggctccaggc ctcggcgcca ccggcgatca ccacctgcgc
      121 ctggcccagg gccagcaggc cgcaggcatg ggtgatggcg tccaggccgg cgcagcattg
      181 gctgtccagc gtgatggcgg gtgtgcgctc gggcaggcct gcggccagcg ccagcacgcg
      241 cgcggggttg ccgcccgcgc ccagtgcgtt gcccagtacc acggcatcca ccgcgctggc
      301 aggcaaccct gcgtcggcca gcaggcgcgc caccagcggg gccgccagct cgtgcggccg
      361 gcaggcagca agcgccccgc ccacgggcgc cacgggcgtg cgcgcccagg ccaggatcgg
      421 catggcgttc acggccaggg ctccagcagg ggcgacgcgc cgacgggcgt atcggccagc
      481 gcgcgggcta tggctccatg gtcggtcttg ccgctggcgg tctgcggcca gtcgcccttc
      541 cacagccacc agtggcgcgg ggttttaaaa ggctccagcg cctctcggca ccaggcggcg
      601 agctgctgcg ctccggcggg cgcatcaccc tgccactgca gcacggcatg cacactgctg
      661 ccgcgcagct catcaaacgc gccctgcagg ctggcctggg ccacggcggg gtgggccagc
      721 aggcgctgct ccacctcttc ggggaacagg ttcttgccct gggtgaccag catgcggttc
      781 tcgcgcccgc acaggtgcaa gaggccggat gcgtccagat ggcccatgtc gcgcaccgac
      841 agccagtcgc ccacgcgcag cgcggcggtg gcgtcggcct ggttcacgta gtccatgaac
      901 agcatggggc tacgcaccca gatcagcccc ggctggcccg cgggcagggc cggcgcctcg
      961 gggtgctcgc ctatgtgcag atcaacgttg ctgaaggccc ggcccacggc ctgagcgggg
     1021 gcggccgggt cggcgtccat ccaggcgatg tagctggcct cggacgcgcc atagaactcg
     1081 acgatgcgcg cgcgcggaaa cagccggcgc agcgccggcg tgcgctcgcg cagccagcgc
     1141 gcgccgctga tcagcaccag ctccacgccc gctatcggcg ccagccggcg gcgctctgcg
     1201 tactgcagca tcagcaccag ctggctgggc acggccacca gcagcggcgc ggcgccggcc
     1261 tgcagcgtgg ccagcgcgcg cggggcggaa aagcgctcct gcagcagcgc gcccgcgccc
     1321 gtccacaggc ccagcagcgc gccaaacaag aacagcgaat gcgacatgcg ccccggcgcc
     1381 agcacgcgcg tggcggcggc ggggccaaag tcgcgcaggc tgacgcggaa gctctcggcc
     1441 caggaacggt ggtggcgcat gaagcctttg ggcaggccgg tgctgcccga ggtgaagccg
     1501 atgtagaacg gcgacagcgg cgtggccccc tgccccggcg cgccggccgg cacgggcagg
     1561 cgctccgcca ccgcctgggc caccacggcc ggccattgcg catcgcccat ggcggcgcag
     1621 cgcccgctgg cgatcacgcc cagaaagtcc accaggccat cgagcgtgcc ggcgtcgccg
     1681 cccaacagca cggtggcggg tgcgccctcg gcgtcgagcc tgcgcgcccg ggcctgcacg
     1741 cgcgcatgca gctcggcaaa actgagccgc tggcgctcat tggccaacgc cagggcatcg
     1801 ggccgggtgc gcgcccagtg cgccagcggc ccgtgcaccc agtcgaagag tgcgggcagc
     1861 ggcccgccgg gccgcagttg tcgtgccagc gcgtgcggcg cgggggccag ggcggcgctc
     1921 aacgctcgcg cccgccaaac ggccagtgcg gcatggcctt ggccacggtg tgcacgacca
     1981 cggcgcagag caggcacttg accaggtcgc cgggcacgaa caccaggtcg gcaaaaaagg
     2041 ccttctccag cggcatcttg gccagcagca ccaggccggc aatgcccatg gcgtgcagga
     2101 acaccaggcc accgagcgcc gaggcaatga aggcgctgat tgccgtgcgc agcggctggc
     2161 cctggggcag caggcgcatc agcgcgcccg tcacgcccgc agccagggcg tagcccagca
     2221 ggtaaccggc cgagggcgcc atgaacaccc ccaggccgcc gcgcccgccc gccagcagcg
     2281 gcaggccggc cgccaccgcc aacagaaaca gagcgatggc ctgaaagccc aggcgcgcgc
     2341 ccagcaggca gccggccagc atcacgccca gggactgcag cgtgatgggc acgcccagcg
     2401 gcaggtcgat cttggggatc agccccatca cggccatcag ggcggcaaac agcgccacca
     2461 gggccatgga gtgggaggcc ttgcgggata cggcggtggt catgacaacg tccttgggtc
     2521 aaagtaaaaa ggtttagccg cccaggcgcg cgtacagggc gtcggccacg cgctgcgcgg
     2581 cctgcaacat ttgcaccgtg agcggcgcaa tcaaacgcag gccgccgggg cggccggtgc
     2641 gcaggcggtg ggcttcatcg agcttttgcc actgcacgaa gaaatgctcg gtaaagcgca
     2701 gcatcagggc cagctgcagc gacagacgct cgggccgcag gcccaggcgc gccagcgggg
     2761 cgagcagcgc atccagcacc aggagcagat cggtggggcg cgtcgtcacc gtgagcgcca
     2821 cgcccagggt ggaggcgcag gccaggcgca gcgcgctcac ggccgccacg tcgggccggc
     2881 cgagccacag gtgaaacccc gccaccagag ccgccgccac cagcaccgag cggatcagcc
     2941 gccgcacctt gtgcgtggcc ggccccaggc tgcaccacag cagcgcgcag gccaggcccg
     3001 ccgccgccag cagccagagc tcgttgaata gaaacagcag cgtgcccagc agcgccatgc
     3061 aggccagctt ggcgccggca ggccagcggt gcagccaggt caggtgttcg ctgtagagac
     3121 tgcccatgaa agaccttcaa gcgacggcgc gcagctccgg gcgcgcggcg ctgcgggcat
     3181 gcgccacctc ggccgccacg gccgcctcgt aggcggcgca gacctcgcgc cccggcccgt
     3241 cgccgcgcac ccggccctcg tgcagccaga tcacgcgggg gtagtcacgc accgggccca
     3301 gcacatgggt ggacaccagc acctgccggc cgcaggcggc aatgtcatcg gccagccgcg
     3361 cctgaccggg cagatccagg ctggcgtagg gctcgtccag cagcagcgtg cgcggcccgg
     3421 ccagcagcag ggccagccag cacagccgct ggcgctggcc ctggctcagc gtgcccacgg
     3481 cgcgctcggc ccagtcggcc aggccacgct cggcgaggaa ggcgcgggcg cgcgccagcg
     3541 cctcgcgccg actcaggccc tggggctgca ggcccaggcc caattcctcc tgcacggtag
     3601 gaaagatgat ctggtcgtcg gggttctgga acatcatgcc cacgcgctgg gcccgcagtt
     3661 gccccgctgg cagcacgtgc aagtcctgcc cctgcgacag cacctggccg ccctgcgggg
     3721 cctccagcgc gcacagcagg cgcagcaggc tggtcttgcc cgcgccgttg tggcctatca
     3781 ggcctatgcg ctactcggcc aggtgcaggt tcagcccggc gaacacctgg ctgccgcccc
     3841 ggcgcaaatg cacctggcgc agctccagcc aggggctggc ggcatcggcg ggcgcatcgg
     3901 cggcaggcgg cattcggttt ttccaaagcg ggtgttgcgc aaattctaac cacatcaaat
     3961 gcgtcaatct cgcacgcaaa aacaggcatt tttcaataac gtctttgatt acgcaggcaa
     4021 tttgcgcgcg caaaagcgtc taactgcagc gaaaattaga cccagatagg caccagttac
     4081 cgacagacag cgcaaacagc aaccaaccaa gctcttcttg ccgtcatttg ccgccttctt
     4141 gcggtcaaca taggccatgg ccgccgcaag aaaatgaatc caagggcgca caataggctc
     4201 tcgccgcgcg ccggccactc cccccttcct ccgtccctgc ccatgaccac aacctcttcg
     4261 acgctggagc gccgcaatct tggcctgctg gtggccgtcc agtcgctggg aggcgcgtcg
     4321 ccacccatca tcatttcgct gggtggcctg gtggggcaga tgctgtcgag cacgccatcg
     4381 gccgccacgc tgccggtcag cctgtaccag ctgggcctgg ccctgtccac gctgccagcg
     4441 gcctggctga tacaccgcgc cgggcggcgc acggcctacc tgctgggggc ggtgatgggc
     4501 atgctgtcgg gcctggtggc cgcctggggc atcagccagt cggacttcat gctgttctgc
     4561 ctgggcacga cgctggccgg tttctatggt gcctgcgtgc aaagctaccg ctttgccgcc
     4621 atcgacgccg tgggcgaccc ggcacggcat gccggcgcca tttcgcgcgt catggtgggc
     4681 ggtctgatcg ccgccgtcat cggcccgcag gtggtgatct ggacgcgcga cagcctgccc
     4741 atgacgccct ttgccggcag cttctacagc caggcggcgc tggcgctgct ggccctgccg
     4801 ctattgctgc tgctgcgcct gccgcccgct cctccaaagg ccgtggccca tggcgcgcgc
     4861 gacctgtccg agatcgtgcg cacgccgcag ttcatcgtcg ccgccacggc gggcgtggtc
     4921 agctacgggc tgatggcctt cctgatgacg gccgcgccca tggccatggt gggctgcggt
     4981 cacagcgtgg gcgaggcggc gctgggcatc cagtggcatg tgctggcgat gttcgggccg
     5041 agcttcttca ccggtcggct catggatcgc tttggcaaac gccgcatcac cgccctgggc
     5101 ctgatcctga tcgccgcctc tggcgcgctg gcgctgatgg gcctggcgct gacgcacttc
     5161 tggggcgccc tggtgctgct gggcctgggc tggaacttcg gcttcatcgg cgccacggcc
     5221 ctgctgaccg aggcctaccg cccgtccgag cgcgccaagg tgcaggcgct caacgacttc
     5281 ctggtgttcg gcaccgtggc cgtggcctcg ttcggctcgg gccagctgct gcacagcgtg
     5341 ggctgggacg gcatcaacct gggcatgctg cccctggtgg tcgtggtgtt ggcgctgctt
     5401 gcgtggcagg ggctgcgcca gcggcaacag atgaccgcac gcggttgagt ttccgaggat
     5461 tcaagtgatg caatcagcca gcgacgaatg gtttgacgag gcgtactacc agcgctacta
     5521 cttcaacaag aaaaccagcg tggtggaccc tgcccacacc gaacgcctgg gagcctttgt
     5581 ctgcagctat ctgcagtacc tgcgcgtgcc cgtggcgcgg gtgctggaca tgggctgtgg
     5641 cataggcctg tggcgtgaca tggtggcgcg gcacctgccg ggcgcgcagt accagggggt
     5701 ggaatacagc gcctatctgt gccagcgctt tggctggcag cgcggctcgg tggtggacta
     5761 cccgtcggcc acgccctatg acttcgtcat ctgccagggc gtgctgccct acctgggcgt
     5821 gcaggacctg cgccgcgcgc tggccaacct gggccggctg tgccagggcg cgctgtacct
     5881 ggaggccgtg accctggaag actacgagcg cggcaccata gacgaagacc tgaccgacgc
     5941 gcgccagcac cgccaccgcg ccgccctgta ccgccagggc ctggcgcggc atttcaccga
     6001 catgggcggc ggcctgtggc tgagccaccg cgcccaggtg ccgctgtttg cgctggaggc
     6061 acgggacggc gcatgaccgc ctcgcccccg cgccgcagcc gctgcccgcg ctgcctgcgc
     6121 ccggatgcaa cctgcctgtg ccccgccgtg cgcagcgtgg ccagccaggt gcaggtgctg
     6181 atcctgatgc acccgcagga agagcaccag gccaagggca cggcgcggct cctgcacctg
     6241 tgcctgccgg cgagccgcct ggccgtgggc gagacctttg cggcgcaaga actggccagg
     6301 ctgttgcaag aaccctggtg cgatgccgat gcccgggcgc cgcgccatgc catgctgctg
     6361 tacccgccca caccgtccga tgcgcagcac cccatgccca caccaccgcc catgccggcc
     6421 gactggctga gcgcgcccga gcggctgcgc ctggtgctgc tggacggcac ctggcgcaag
     6481 agccgcaaga tgctgtggag caaccccacg ctgcaggccc tgccgcgcct ggcgctgcac
     6541 catgtgccgg cctcgcgcta cgcgatccgc aaggcgcacg caccgcacca gctatcgaca
     6601 ctggaggcca cgcaacaggc gctgcggcat ctggagcccg ccaacgccgg tattgccgcg
     6661 ttgggcgccg ccatgcagga cttcatggcc cggcagcagg cctactggcc cgctgcgcac
     6721 tggactatgc tcaacgcaac cgactcttga accccggaga cagccatgac ccagcgctac
     6781 cccctgcccg acctgaacac cctgcccgag gacatccgca cgcgcatcct ggaggtgcag
     6841 gacaaggccg gcttcatccc caacgtattc ctggccttcg cacgccgccc ggacgaatgg
     6901 cgcgccttct ttgcctacca cgacgcgctg atgctgcgcg agcaaggcag cctgaccaag
     6961 ggcgagcgcg agatgatcgt caccgccacc agcgccgcca accagtgcct gtactgcgtg
     7021 gtggcccatg gcgccatcct gcgcatctat gagaaaaacc cgctggtggc cgaccaggtg
     7081 gccgtgaacc accgcaaggc cgacatcagc gcgcgccagc aggccatgct ggactttgcg
     7141 atgaaggtct gcctgcgcgc gcaggagatt gacgatgccg acttcgaggc gctccacccc
     7201 cacggcttcg acgacgaaga catctgggac atcgccgcca tcaccgcctt ctttggcctg
     7261 tccaaccgca tggccaactt ttccggcatg cagcccaacc ccgagttcta tctgatgggc
     7321 cgcatgccgc gcgagaagaa gg
//
LOCUS       DAOMTQ010000142         7338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2306_length_7338_cov_26.319648, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000142 DAOMTQ010000000
VERSION     DAOMTQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2306_length_7338_cov_26.319648"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PK518_12360"
     CDS             complement(<1..323)
                     /locus_tag="PK518_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRM49751.1"
                     /translation="MALIRSLLHLLVMIITVIPYTTVILLVRLLGGSPAARYRVARAW
                     LALCVDSARWIVGIRTRVEGMEHLPRDSTQGAVLLVKHQSAFETFLMPAIMPHALAYV
                     FKKELL"
     gene            complement(561..1136)
                     /gene="gmhB"
                     /locus_tag="PK518_12365"
     CDS             complement(561..1136)
                     /gene="gmhB"
                     /locus_tag="PK518_12365"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HRM49752.1"
                     /translation="MKLAILDRDGTLNALGDGFIASAADWTPLPGALEAVARLNHGGW
                     HVVLATNQPGLGRGLFDAQTLVAIHAKMHRQLAALGGRIDAVFYCPHAETEGCNCRKP
                     APGLMEQIRERYGADGDEILVVGSCPAHLQAASELGAQLHMVCTGRSAHLDPLQPLPP
                     GWPPGTRAHASLGALVDYLLPQGEAGLPAAL"
     gene            complement(1150..1752)
                     /locus_tag="PK518_12370"
     CDS             complement(1150..1752)
                     /locus_tag="PK518_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49753.1"
                     /translation="MAMAQAAAPGAGQRGAWLLFAPLAAWVGLRWVLQWLQDRAPQPA
                     AWPITPFVGAQDPWAWLRPAGMAIAALALLALLARAWARRHGWRAPLRLLTAAWLLLW
                     TAACAAQLQSFLNLRALVPQAPLQAELLGAHAQPPSQRSLGGQLWVLRIAGEDAPQQV
                     LVPDAAAAQVPLHARLNLRWARGRWHGRYLSGWDMLPVQP"
     gene            complement(1752..3872)
                     /gene="glyS"
                     /locus_tag="PK518_12375"
     CDS             complement(1752..3872)
                     /gene="glyS"
                     /locus_tag="PK518_12375"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRM49754.1"
                     /translation="MTHSNLLVELFVEELPPKALQKLGDAFAGVLREQLATQGLCAAD
                     TPLTAYASPRRLAAHIGNVLDQAPDKAVSQKLMPVAVGLGSDGLPTPALLKKLAALGA
                     DASAVAGLKRVHDGKAELLFYESQARGATLAQGLQKALDEAIARLPIPKVMRYQLQDG
                     WNSVNFVRPAHGLVALHGSAVVPVTVLGLAVGNSTHGHRFEAAVDPVVIHDADSYAAQ
                     LAEQGAVIASFAERRAEIARQLQAAADKIGGGVRPIADDALLDEVTALVERPNVLVCQ
                     FETEFLAVPQECLILTMKANQKYFPLLDASDKLTHQFLIVSNIRPQDASAVIQGNERV
                     VRPRLADAKFFFDQDRKKTLQSRVAQLAKVVYHNQLGTQGERVERVRAIARAIAARLG
                     GAQLAQQADQAALLAKTDLVTDMVGEFPELQGTMGRYYALNDGLDAAVADAIEDHYKP
                     RFAGDALPRGAAGVVVALADKLETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLVEK
                     DLPLDLIELVALAQDKFGDKLSGVDAIQGSLQLVDFIYDRLAGSLREQGYSAQEVDAV
                     LALRPQRLALVQKQLAAVRAFSALPEAPALAAANKRITNILKKAEAVDAHVNPELLQE
                     QAEQDLYAALQRFVPEADAQFAAGDYTASLQTLAVLRAPVDAFFDDVMVNAEQLDLRL
                     NRQGLLKSLHDAMNRVADLSRLAA"
     gene            complement(4012..4950)
                     /gene="glyQ"
                     /locus_tag="PK518_12380"
     CDS             complement(4012..4950)
                     /gene="glyQ"
                     /locus_tag="PK518_12380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRM49755.1"
                     /translation="MLTFQQIILKLQTYWADQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPAPANILELYLGSLEALGF
                     DLKKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPATGEITY
                     GLERLAMYLQGVDNVYNLTWTDGLTYGDVYHQNEVEQSTYNFEHSDAEFLFTAFGAHE
                     KQAQHLMGEQLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNLARAVGKSY
                     LDSRARLGFPMAPKAHADEVLAELAKAAEQQQKKAA"
     gene            complement(5055..5867)
                     /locus_tag="PK518_12385"
     CDS             complement(5055..5867)
                     /locus_tag="PK518_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRM49756.1"
                     /translation="MSYLSPAEFVTKMVDTGEAKIHMSTRDTLIRAFMAGAILALAAV
                     FAVSASVMSGSALIGALLFPVGVCMLYLLGFDLLTGVFMLTPLAWLDKRPGASIRGIL
                     RNWALVFVGNFLGALTVAGMMAFIFTYGFSVPPNEIGQKIGHIGEARTLGYAQYGLMG
                     WVTIFLRGVLCNWMVSTGVVGAMISTTVSGKVIAMWMPIMLFFAMGFEHSVVNMFLFP
                     SGLMLGGNFSLMDYLVWNEIPVVLGNLVGGLAFTGLTLYTTHARTAPQRALG"
     gene            complement(5889..6272)
                     /gene="nirD"
                     /locus_tag="PK518_12390"
     CDS             complement(5889..6272)
                     /gene="nirD"
                     /locus_tag="PK518_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HRM49757.1"
                     /translation="MKPDWTYICQLNDIPRQGARRVARPQGLEVALFRTGQDAVYALL
                     DHSPYKGGPLSQGMVFGANVACPLHNWTIDLASGQALAPDEGSTPVFRVQVEAGAVYL
                     RTDELASHALTQKRPLAGPARRATT"
     gene            complement(6486..>7338)
                     /locus_tag="PK518_12395"
     CDS             complement(6486..>7338)
                     /locus_tag="PK518_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HRM49758.1"
                     /translation="CRPAINYYLISTWPKEALDDPQSRAINERSHANIQKDGTYSVVP
                     RMWGGETSAAELRRIADVVDKYKIPGVKVTGGQRIDLLGVKKEDLQGVWNDIAMPCGH
                     AYGKSLRTVKTCVGSEWCRFGTQDSSQLGRDLERALWRMYAPHKVKLAVSGCPRNCAE
                     SGIKDVGIIGVDSGWEIHIAGNGGIKTEVAHFFTKVKTAEEVMEVSGAFLQLYREEGW
                     YLERTTHYVARVGLEHVKKKVLEDEANRQVLWARLQAALDGEPDPWFEGEKARVDARQ
                     FIPIAVA"
BASE COUNT         1158 a         2428 c         2512 g         1240 t
ORIGIN      
        1 agcagctcct tcttgaacac ataggccagg gcgtggggca tgatggccgg catcaagaac
       61 gtctcgaacg ccgactggtg cttgaccagc agcaccgcgc cctgcgtgct gtccctgggc
      121 agatgctcca tgccttcgac gcgcgtgcgt atgccgacga tccagcgcgc cgagtccacg
      181 cacagcgcca gccaggcgcg cgccacgcgg tagcgcgccg ccgggctgcc acccagcagg
      241 cgcaccagca ggatgaccgt ggtgtaggga atgacggtga tgatcatcac cagcaggtgc
      301 agcagcgagc gaatcaaggc catgggtggg tagttttcag tgtttttgcc tgattaggca
      361 catgcttcag atacggttgc cagccctcac ccccaccctc tcccagaggg agagggagta
      421 aaaccggcgc aaccggtcgc cctcgcccct ttggggagag ggaggggtga ggggcctctg
      481 cggcatgttc atagtcaggt gtttttggcc tttggcgctt atccataaag cgcaaacagc
      541 tacaaactta atagtaaaca tcacaaggcg gcgggcaggc ccgcctcgcc ctgcggcaac
      601 agatagtcca ccagcgcccc caggctggca tgggcgcgag tgccgggggg ccagcctggc
      661 ggcagcggct ggagcgggtc caggtgggcc gagcggccgg tgcacaccat atgcagctgc
      721 gcgcccagct cgctggcggc ctgcagatgc gccgggcagc tgccgacaac caggatctcg
      781 tcgccatcgg cgccatagcg ctcgcggatc tgctccatca ggccgggggc gggcttgcgg
      841 cagttgcagc cctcggtctc ggcgtgcggg cagtagaaca ccgcgtctat gcgcccgccc
      901 agcgcggcga gctggcggtg catcttggcg tggatggcga ccagggtctg ggcgtcgaac
      961 aggccgcggc ccaggcccgg ctggttggtg gccagcacca catgccagcc gccatggttc
     1021 agccgcgcca cggcctccag cgcgccgggc aggggcgtcc agtcggcggc cgaggcgatg
     1081 aagccgtcgc ccagggcgtt cagggttccg tcacggtcga ggatggcgag tttcatggcc
     1141 gcagcactct caaggttgga cgggcagcat atcccagccg ctcaggtagc gtccatgcca
     1201 gcgcccacgc gcccagcgca ggttcaggcg cgcatgcagc ggcacctgag cggcggcggc
     1261 gtccggcacc agcacctgct gcggcgcgtc ctcgccagcg atgcgcagca cccacagctg
     1321 gccgcccagc gagcgctgcg acggcggctg ggcatgggcg cccaacagct cggcctgcaa
     1381 cggagcttgc ggcacgaggg cgcgcaggtt caagaacgat tgcagctgcg ccgcgcaggc
     1441 cgccgtccac agcagcagcc aggcggccgt cagcagccgc agcggcgcac gccagccatg
     1501 gcggcgcgcc caggcgcgtg ccagcagggc cagcaatgcc agggcggcga tggccatgcc
     1561 cgccgggcgc agccaggccc agggatcttg cgcgccgacg aagggggtga tgggccaggc
     1621 tgcgggttgg ggcgcgcgat cctgcagcca ctgcagcacc cagcgcaggc ccacccaggc
     1681 ggccagcggg gcaaacagca gccaggcgcc gcgctgccct gcccccggcg ccgccgcctg
     1741 cgccatggcc atcaggcggc caggcgcgag aggtcggcga cgcggttcat ggcgtcgtgc
     1801 aggctcttga gcagcccctg gcgattcagg cgcagatcca gctgctcggc attcaccatc
     1861 acgtcatcga agaaggcatc caccggcgcg cgcagcacgg ccagggtctg caggctggcg
     1921 gtgtaatcgc cggcggcaaa ctgcgcgtcg gcctcgggca cgaagcgctg cagggccgcg
     1981 tacagatcct gctcggcctg ctcctgcagc aactcggggt tgacatgggc atccaccgcc
     2041 tcggccttct tcaggatgtt ggtgatgcgc ttgttggccg ccgccagggc cggggcctcg
     2101 ggcagcgcgg agaaggcgcg caccgccgcc agctgctttt gcaccagggc caggcgctgc
     2161 gggcgcaggg ccagcacggc gtccacctcc tgggcgctgt agccctgctc gcgcagggag
     2221 ccggccaggc ggtcgtagat aaagtcaaca agctgcaacg atccttgaat ggcatcgacg
     2281 ccactcagtt tgtcgccaaa cttgtcctga gccagcgcca ccagttcgat cagatccagc
     2341 ggcaagtcct tctcgaccag catgcggatc acgcccagcg cgtggcggcg cagcgcgaag
     2401 gggtcgcggt cgccggtggg caggttgcct atgccgaaca tgccgaccag ggtctccagc
     2461 ttgtcggcca gggccacgac cacgccggcc gcgccgcgcg gcagcgcatc gccggcaaag
     2521 cgcggcttgt aatggtcttc gatggcgtcg gccaccgccg cgtccaggcc gtcgttgagc
     2581 gcgtagtagc ggcccatggt gccctgcagc tcggggaact cgcccaccat gtcggtgacc
     2641 aggtcggtct tagccagcag cgcggcctgg tcggcctgct gcgccagctg ggccccgccg
     2701 agccgcgcgg caatcgcccg ggcgatggcc ctgacgcgct ccacgcgctc gccctgggtg
     2761 cccagctggt tgtggtagac caccttggcc agctgcgcga cgcggctttg cagggtcttt
     2821 ttgcggtcct ggtcgaagaa gaacttggca tcggccaggc gcgggcgcac cacgcgctcg
     2881 ttgccctgga tgacggcgct ggcgtcctgc ggccggatgt tgctgacgat gaggaattgg
     2941 tgcgtcagct tgtccgaggc gtccagcagc ggaaagtact tctggttggc cttcatggtc
     3001 aggatcaggc attcctgcgg cacggcgagg aattccgtct cgaactggca gaccagcacg
     3061 ttggggcgtt cgaccagggc cgtcacctca tcgagcagcg catcgtccgc tatggggcgc
     3121 acgccgccgc caatcttgtc ggccgccgcc tgcagctggc gcgcgatctc ggcgcggcgc
     3181 tcggcaaagc tggcgatcac cgcgccctgc tcggcgagct gcgcggcgta gctgtcggca
     3241 tcgtggatca ccaccgggtc cacggccgcc tcgaagcggt ggccatgcgt gctgttgccc
     3301 accgccaggc caagcaccgt caccggcacc acggcgctgc cgtgcagcgc caccaggcca
     3361 tgggcggggc gcacgaagtt cacgctgttc cagccgtcct gcagctggta gcgcatgacc
     3421 ttgggaatgg gcagcctggc aatggcttcg tccagcgctt tttgcaggcc ctgggccagc
     3481 gtggcaccgc gtgcctggct ctcgtagaac agcagctcgg ccttgccgtc atgcacgcgc
     3541 ttgagacccg ctacggccga ggcgtcggcg cccagcgcgg ccagtttctt gagcagcgcc
     3601 ggcgtgggca agccatcgct gcccagaccc acggccacgg gcatgagctt ttgcgacacg
     3661 gccttgtcgg gcgcctggtc cagcacgttg ccgatatgcg cggccaggcg gcgcggcgag
     3721 gcgtaggcgg tgagcggcgt gtcggccgcg cacaggccct gggtggcaag ctgctcgcgc
     3781 agtacgccgg caaaggcgtc gccgagcttt tgcagggcct tcggaggcag ttcttcgacg
     3841 aagagttcaa caagaaggtt tgaatgggtc atggtgtgtg atcttgtctg actccctctc
     3901 ccgctggcgg gagagggtgg gggtgagggt gggctcgggc tgggcgctgg gccctggcac
     3961 cccctcaccc ctgccctctc ccccaagggg gagagggagt gtggttgctc ctcaagccgc
     4021 ttttttctgc tgctgctcgg ccgccttggc cagctccgcc agcacctcat cggcatgcgc
     4081 cttgggcgcc atggggaaac ccaggcgcgc gcggctgtcc aggtaactct tgcccacggc
     4141 gcgcgccagg ttgcggatgc ggccaatgta ggcggcgcgc tcggtaacgc tgatcgcacc
     4201 gcgtgcatcc agcagattga aggtgtgcgc ggccttaagc acctgctcgt aggccggcag
     4261 cgccagctgc tcgcccatca aatgctgggc ctgcttttca tgcgcgccaa aggcggtgaa
     4321 caaaaactcg gcatccgaat gctcgaagtt gtaggtggac tgctcgacct cgttctggtg
     4381 gtagacgtcg ccataggtca ggccatccgt ccaggtcagg ttgtagacgt tgtccacgcc
     4441 ctgcaggtac atggccaggc gctccaggcc gtaggtgatc tcgcccgtgg cgggcttgca
     4501 gtcgatgccg ccgacctgct ggaaataggt gaactgcgtc acctccatgc cgttgagcca
     4561 cacctcccag cccaggcccc aggcgcccag cgtggggttc tcccagtcgt cctcgacgaa
     4621 gcggatgtcg ttcttcttca ggtcaaagcc cagggcctcc aggctgccca ggtacagctc
     4681 caggatattg gccggcgcgg gcttaagcac cacctggtac tggtaatagt gctgcaagcg
     4741 attggggttg tcgccatagc gcccgtcctt gggccggcgg ctgggctgca cgtaggcggc
     4801 tttccagggc tctggcccca atgctcttaa aaacgtagct gtatgcgacg taccggcgcc
     4861 gacttccatg tcgtatggct gcaaaagcgc gcaaccctgg tcggcccagt aggtttgcag
     4921 tttgaggatg atttgttgga aggtgagcat ggcggggggc gattcggtaa gggcgtgcgg
     4981 ccgggcgcac gcgaaccggt cattttaggc gctgcaaccg cgctggcttt ccccccggcc
     5041 tttcgttcag cggctcagcc cagcgcccgc tgcggcgcgg tgcgcgcatg cgtggtgtag
     5101 agcgtcaggc cggtgaaggc caggccaccc accagattgc ccagcaccac cgggatttcg
     5161 ttccagacaa gatagtccat cagcgagaag ttgccgccca gcatcagccc cgaggggaac
     5221 agaaacatgt tcaccaccga atgctcgaag cccatggcaa agaacagcat gatgggcatc
     5281 cacatggcga tgaccttgcc gctgacggtg gtggagatca tggcgccgac cacgccggtg
     5341 gagaccatcc agttgcacag cacgccgcgc aaaaagatgg tgacccagcc catcagcccg
     5401 tactgcgcgt agcccagggt gcgggcctcg ccgatatggc cgatcttctg cccgatctcg
     5461 ttgggcggca cggaaaagcc ataggtgaag atgaaggcca tcatgcccgc cacggtcagc
     5521 gcgcccagga agttgccgac gaacaccagc gcccagttgc gcaaaatgcc acgaatgctg
     5581 gcgccagggc gcttgtccag ccaggccagg ggcgtgagca tgaacacgcc ggtgagcagg
     5641 tcaaagccca gcaggtagag catgcacacg cccaccggga acagcagcgc gccaatcagc
     5701 gccgagccgc tcatgacgct ggcgctcacg gcaaacaccg ccgccagcgc caggatggcg
     5761 ccggccatga aggcgcggat caaggtgtcg cgcgtggaca tgtggatctt ggcttcgccg
     5821 gtatcgacca tcttggtgac gaattcggca gggcttaggt aagacatgtg gtgctcctgg
     5881 tggttgggtt aggtcgtggc gcgccgcgcc ggtccggcca gcgggcgttt ttgcgtcagg
     5941 gcgtggctgg ccagctcgtc ggtgcgcagg tagaccgccc cggcctcgac ctgcacgcga
     6001 aacaccggcg tgctgccctc gtcgggcgcc agcgcctggc cgctggccag gtcaatcgtc
     6061 cagttgtgca gcgggcaggc gacgttggcg ccaaacacca tgccctggct cagtgggccg
     6121 cccttgtagg ggctgtggtc gagcagggcg tagacggcgt cctggccggt gcgaaagagc
     6181 gccacctcca gcccctgcgg gcgcgccacg cggcgcgcgc cctggcgcgg gatgtcgttg
     6241 agttggcaga tgtaggtcca atcgggcttc atattgctat acctttcata gttgtttgcg
     6301 cttgtgtggt gggcgctgga gtctgttttg tttaaaaaaa tagcggtcgc tggcggtatt
     6361 tctccctctc ccgctggcgg gagagggtgg gggtgggggt gagggggctt gacgtgggcc
     6421 tggcgcttca tcaccccctc acccctgccc tctcccccaa gggggcgagg gagtgaatac
     6481 cgggctcagg cgacggcaat ggggatgaac tggcgcgcgt ccacccgcgc cttctcaccc
     6541 tcgaaccacg ggtcgggctc gccgtccagc gccgcttgca ggcgcgccca caggacctgg
     6601 cggttggcct cgtcttccag cacctttttc ttcacatgct ccaggccgac gcgcgccacg
     6661 tagtgggtgg tgcgctccag gtaccagcct tcttcgcggt agagctgcag gaaggcgccg
     6721 ctgacctcca tcacctcctc ggcggttttc accttggtga agaagtgcgc cacctcggtc
     6781 ttgatgccgc cgttgccggc gatgtggatc tcccagcccg aatccacgcc gatgatcccg
     6841 acgtccttga tgccgctctc ggcgcagttg cgcgggcagc cgctgaccgc cagcttgacc
     6901 ttgtgcggcg cgtacatgcg ccagagcgcg cgctccaggt cgcgcccgag ttggctgctg
     6961 tcctgcgtgc caaagcggca ccattcgctg cccacgcagg ttttcaccgt gcgcaggctc
     7021 ttgccgtagg cgtggccgca gggcatggcg atgtcgttcc acacgccctg caggtcttcc
     7081 ttcttcacgc ccaagaggtc tatgcgctgc ccgcccgtga ccttgacgcc ggggattttg
     7141 tatttgtcca ccacgtcggc aatgcggcgc agctctgccg cgctggtctc gccgccccac
     7201 atgcgcggca ccacgctgta ggtgccgtct ttttggatgt tggcgtggct gcgctcgttg
     7261 atggcgcggc tttgcgggtc gtccagcgcc tctttcggcc aggtgctgat caggtagtag
     7321 ttgatggccg ggcggcag
//
LOCUS       DAOMTQ010000143         7298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2323_length_7298_cov_26.941599, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000143 DAOMTQ010000000
VERSION     DAOMTQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_7298_cov_26.941599"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="PK518_12400"
     CDS             complement(<1..1865)
                     /locus_tag="PK518_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRM49759.1"
                     /translation="MKFRFPIVIIDEDYRSENTSGLGIRALAQAIEAEGFEVLGVTSY
                     GDLSQFAQQQSRASAFILSIDDEEFTLGTGQDPIVHSLRAFIEEVRRKNVEVPIYVHG
                     ETKTARHLPNDILRELHGFIHMFEDTPEFVAKHIIREAKSYLEGVQPPFFKALLDYAE
                     DGSYSWHCPGHSGGVAFLKSPVGQMYHQFYGENMLRADVCNAVEELGQLLDHNGAIGE
                     SERNAARIFNADHCFFVTNGTSTSNKIVWHHAVAPGDVVVVDRNCHKSILHSIIMTGA
                     IPVFLKPTRNHFGIIGPIPQSEFEPATIQEKIRANPLLKDVDAAAIKPRVLTLTQSTY
                     DGVLYNTQTIKGMLDGYVDNLHFDEAWIPHAAFHPFYGTFHAMGKKRARPQHSVVYAT
                     QSVHKLLAGISQASHVLVQDSQTTKLDRHLFNEAYLMHTSTSPQYSIIASCDVAAAMM
                     EPPGGTALVEESIVEALDFRRAMRQVAEEFGKDDWWFKVWGPEELVDEGLGTAENWVF
                     RSKGKKKNGAANASKWHGFGDLADGFNMLDPIKSTIVTPGLNLDGKFGKTGIPASIVT
                     KYLAEHGVVVEKTGLYSFFILFTIGITKGRWNTLLAALQQFKDDYEKNQPMWRILP"
     gene            2013..2444
                     /locus_tag="PK518_12405"
     CDS             2013..2444
                     /locus_tag="PK518_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRM49760.1"
                     /translation="MNLNPATLWWLMAGVTVAIELLSGTFYLLMLAIGMAAGAVAAHM
                     GLGLTMQIVTCAVVGASTVLVAYIKRRSRPGEPSPRAERSVNLDVGETVFIDAWQADG
                     TAQVKYRGANWTAIHRPGITPSVGAHRVAELVGNRLLVDPL"
     gene            2472..3383
                     /locus_tag="PK518_12410"
     CDS             2472..3383
                     /locus_tag="PK518_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HRM49761.1"
                     /translation="MEVAIVLFVIAVIFIARAVKIVPQQHAWVKERLGKYAGTLSPGP
                     KFIIPFIDRIAYKHSLKEIPLDVPSQVCITRDNTQLQVDGILYFQVTDPMRASYGSSN
                     YISAVTQLAQTSLRSVIGKLELDKTFEERDLINAKVVEAIDEAALNWGVKVLRYEIKD
                     LTPPAEILRAMQAQITAEREKRAVIATSEGKRQEQINLAEGERQAFIAKSEGEKQAAI
                     NNAEGEAAAITAVADATAGAIERIAAAIRQPSGDQAVQLKVAEKAVDAYSKVAADATT
                     TLIVPSNMTEVSALVASAMKMIQTSQR"
     gene            complement(3387..4295)
                     /locus_tag="PK518_12415"
     CDS             complement(3387..4295)
                     /locus_tag="PK518_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49762.1"
                     /translation="MTILRKTFLPPIADLLAFEAAARHASISRAAQELHLTQSAVSRQ
                     IRQLEQQLGMALFHRVRQRIVLTDAGRVYAADVRAALQQLSASTQKAMALSHAGGLLN
                     LAVLPTLGTRWLIPRLGSFTAQHPDVTVNCAARTEPFDFAGTPFDAAIHFGAPTWPGA
                     VCEFLMREEVVPVASPAFRQGHGIRSPQDLTRVVLLQQSTRPTQWAEWFEQVGAPTTV
                     ALRGPRLEHFAMIAQAAVCGQGVALLPRFLIAQELASAGLVQLFDDAVVSADAYYLVY
                     PEARAQTPLVMAFRDWLLVQCAGSQE"
     gene            4511..5422
                     /locus_tag="PK518_12420"
     CDS             4511..5422
                     /locus_tag="PK518_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM49763.1"
                     /translation="MASSYTKMAATAAGLALWCAALPAALAQAPSATLDKIKASGKAV
                     LGVRESSPPMAYALGANEKYVGYHVELCERVLKEIAPTAKLEYMAITAQSTMPLVSNG
                     TVDIGCGPTTNNLARQQQVAFAVTSYVSEVRMAVRKDSDLTSIAQLGGRTVAASTGTT
                     AVQLLRKQERVVGSPIKTVLGKDHHESFMLLESGRADAFVLDDNILAGVIANSKDPSA
                     YRIVGEPLGAEPIALLFRKDDPGFKAAVDGVLTRLMQSGEMEKIYTKWFVNPIPPKNA
                     SLNLPLGNALRQLFATPNDKPLETYQQ"
     gene            5431..7113
                     /locus_tag="PK518_12425"
     CDS             5431..7113
                     /locus_tag="PK518_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRM49764.1"
                     /translation="MSPALTPRTGGQILVRQLITHGVQQLFCVPGESYLAVLDALHDA
                     DIAVTVCRQEGGAAMMAEAQGKLTGRPGICFVTRGPGATNASAGVHIAHQDSTPMILF
                     VGQVARGAKGREAFQELDYRAVFGTMAKWVVDIDDPARLPELISRAFHVATSGRPGPV
                     VVALPEDMLTEAASVADALPYQVTETCPGTAALQELHARLQGAEQPVVILGGSRWSDQ
                     AVRDIATFAEHWQLPVYCSFRRQMLFPANHASYAGDLGLGVNPKLLARMRASDLVLVL
                     GARLSEIPSQGYELFDIPNPAQPLVHVHADADELGRLYRPAQTIHATPQAMAAALVSL
                     LPATAPGWAGHTQAARAEYLAWSDPAPIRIPGQLQMGAVMQHLKGVLPADTIFCNGAG
                     NFATWVHRFWPFTSYGSQLAPTSGSMGYGLPAGVGAKRLWPAREVVVFAGDGDFLMHG
                     QEFATAVQYGLPLVVVLLDNAMYGTIRMHQEREYPGRVSATALKNPDFKAYAQAFGGH
                     GERVERTEDFAPALERARASGLPAILHCFIDPEAITPTGTLQGIRNAALTNG"
     gene            7206..7296
                     /locus_tag="PK518_12430"
     tRNA            7206..7296
                     /locus_tag="PK518_12430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7240..7242,aa:Ser,seq:gga)
BASE COUNT         1373 a         2440 c         2254 g         1231 t
ORIGIN      
        1 ggaaggatgc gccacatcgg ctggttcttc tcgtagtcgt ccttgaactg ctgcagcgcg
       61 gccagcagcg tgttccagcg acccttggtg atgccaatgg tgaacaggat gaagaagctg
      121 tacagccccg tcttctcgac caccacgccg tgctcggcca ggtacttggt gacgatggag
      181 gccggaatgc cggtcttgcc aaattttccg tccaagttca ggccgggggt gacgatggtg
      241 gacttgatcg gatccagcat gttgaagcca tcggccagat cgccaaaacc gtgccacttg
      301 ctggcattgg cggcgccgtt cttctttttg cccttgctgc ggaagaccca gttctcggcc
      361 gtgcccaggc cctcatccac gagctcctcg gggccccaga ccttgaacca ccagtcgtcc
      421 ttgccgaatt cctcggccac ctggcgcatg gcgcggcgaa agtccagcgc ctcgacgatg
      481 ctttcctcca ccagcgccgt gccgccgggc ggctccatca tggcggccgc cacgtcacag
      541 ctggcgatga tgctgtactg cgggctggtc gaggtgtgca tcaggtaggc ctcgttgaac
      601 aggtggcggt cgagcttggt ggtctggctg tcctgcacca gcacgtgcga ggcctggctg
      661 atgccggcca gcagcttgtg caccgactgc gtggcataga ccaccgaatg ctgtggccgc
      721 gcgcgcttct tgcccatggc atggaaggtg ccgtagaacg ggtggaaggc cgcatgcgga
      781 atccaggcct cgtcaaagtg caggttatcg acatagccat cgagcatgcc cttgatggtc
      841 tgggtgttgt agagcacgcc gtcgtaggtg gactgcgtca gcgtcagcac gcgcggcttg
      901 attgcggccg catccacatc cttgagcaag gggttggcgc ggatcttttc ctggatggtg
      961 gctggttcga actcgctttg cggtatcggg ccgatgatgc cgaaatggtt gcgcgtgggc
     1021 ttaaggaaca ccggaatggc cccggtcatg atgatgctgt gcagtatcga cttgtggcag
     1081 ttgcgatcca ccaccaccac gtcgcccggc gccaccgcgt ggtgccagac gatcttgttg
     1141 gacgtgctgg tgccattggt gacgaagaag cagtggtcgg cgttgaagat gcgcgccgca
     1201 ttgcgctcgg actcgccaat cgcgccgttg tggtccaaca gctggccgag ttcttccacc
     1261 gcgttgcaca cgtcggcgcg cagcatgttc tcgccataga actggtggta catctggccg
     1321 accgggctct tcaaaaacgc cacgccaccc gagtggcctg ggcagtgcca gctgtaggag
     1381 ccatcctcgg catagtccag cagcgccttg aagaacggcg gctgcacccc ctccaggtag
     1441 ctcttggcct cacggatgat gtgcttggcc acaaactcgg gcgtgtcctc gaacatgtgg
     1501 atgaagccgt gcagctcgcg caagatgtcg ttgggcaggt ggcgcgcggt cttggtctcg
     1561 ccgtgcacgt agatcggcac ctcgacgttc ttgcgccgca cctcctcgat gaaggcgcgc
     1621 aagctgtgca cgatgggatc ctgccccgtg cccagggtga actcctcgtc gtcgatggac
     1681 aggatgaagg cgctggcgcg gctttgctgc tgggcaaatt ggctcaggtc gccatagctg
     1741 gtcacgccca gcacctcgaa gccctcggcc tctatggcct gggccagcgc cctgatcccc
     1801 aggccggagg tgttttctga acgatagtcc tcgtcgatga tgacgatggg aaagcgaaat
     1861 ttcatgcgct gcctccggca accgggtcaa taaacaaaat aggcgcgaag tgtatgaaaa
     1921 atgggcttgt tgcctatgaa actgctggta tttcgcccaa aattcgtcgc actggaaaca
     1981 aaaaaccaca atacgagcca aggagataca acatgaacct caatcccgcc actctctggt
     2041 ggctgatggc aggtgtcacc gtggccatag agctgctcag cggcaccttc tacctgctga
     2101 tgctggccat aggtatggcc gcaggcgccg tggcagcgca catgggcctg ggcctgacga
     2161 tgcagatcgt gacctgcgcc gtggtcgggg ccagcacggt gctggtggcc tatatcaagc
     2221 gccgctcacg cccgggcgaa cccagccccc gggccgagcg cagtgtgaac ctggacgtgg
     2281 gcgaaaccgt cttcatcgac gcctggcagg ccgacggcac cgctcaggtc aaataccgcg
     2341 gcgccaattg gactgccatc caccgtcctg gcatcacccc gtcggtcggc gctcatcgcg
     2401 tggccgagct ggtgggcaac cggctgctgg tcgatccgct gtaacttgaa atcacagaaa
     2461 gaataccccc catggaagtc gccatcgtcc tcttcgtcat cgccgtgatc ttcatcgccc
     2521 gcgccgtcaa gatcgtgccc cagcagcatg cctgggtgaa ggagcgcctg ggcaagtacg
     2581 cgggtaccct ctcgcccggc ccgaagttca tcattccctt catcgatcgc attgcctaca
     2641 agcacagcct gaaggaaatt ccgctggacg tgcccagcca ggtgtgcatc accagggaca
     2701 acacgcagct gcaggtggac ggcatcctgt actttcaggt caccgacccg atgcgcgcca
     2761 gctacggctc aagcaactac atcagcgccg tgacgcagct ggcgcagacc tcgctgcgct
     2821 cggtcatcgg caagctggag ctggacaaga cctttgagga gcgcgacctg atcaatgcca
     2881 aggtggtcga agccatcgac gaggcggcgc tcaactgggg cgtgaaggtg ctgcgctacg
     2941 agatcaagga cttgacgccc ccagccgaga tcctgcgcgc catgcaggcg cagatcaccg
     3001 ccgagcgcga aaagcgcgcc gtgatcgcga catcggaggg caagcgccag gagcagatca
     3061 acctggccga gggcgagcgc caggccttca tcgccaagtc ggagggcgag aagcaggccg
     3121 ccatcaacaa cgccgagggc gaggccgccg ccatcaccgc cgtggccgat gccaccgccg
     3181 gcgccatcga gcgcatcgcc gccgccatcc gccagcccag cggcgaccag gcggtgcagc
     3241 tcaaggtggc cgagaaggcc gtcgatgcct acagcaaggt cgccgccgac gccaccacta
     3301 ccctcatcgt gcccagcaac atgaccgagg tctcggccct ggtggcgtca gccatgaaga
     3361 tgatccagac cagccaacgt tgatgcctac tcctgactac ccgcgcactg caccagcagc
     3421 cagtctctga aggccatgac cagcggcgtc tgcgcacgcg cttcggggta gacaaggtag
     3481 taggcgtcgg cgctcacaac cgcatcgtca aacaattgca ccaggccggc gctggccagc
     3541 tcctgcgcga tcaagaagcg cggcaacagg gccacgccct ggccacacac cgcggcctgg
     3601 gcaatcatgg cgaaatgctc caggcgcggg ccgcgcagcg ccacggtggt gggtgcgccc
     3661 acctgttcaa accactcggc ccattgcgtg gggcgcgtgc tttgctgcaa cagcaccacg
     3721 cgcgtcagat cctgcgggct gcgtatgcca tgcccctggc gaaaggctgg gctggccacg
     3781 ggcaccacct cttcgcgcat caggaactcg cacacggcgc caggccaggt gggggcgcca
     3841 aaatggatgg cggcatcaaa aggcgtgccg gcaaagtcaa acggctcggt gcgcgctgca
     3901 cagttcacag ttacatcagg gtgctgcgcc gtgaacgatc ccaggcgcgg tatcagccag
     3961 cgcgtgccca gcgtgggcag cacggccagg ttcagcaggc cgccggcatg cgagagcgcc
     4021 atggccttct gcgtgctggc cgacagctgc tgcagcgctg cgcgtacatc ggcggcgtag
     4081 acacggccgg cgtcggtgag cacgatgcgc tggcgcacgc ggtgaaacag cgccatgccc
     4141 aactgctgct ccagctgccg tatctggcgc gacacggcgc tttgcgtcag gtgcagctcc
     4201 tgcgccgcac gcgagatgct ggcgtgccgc gccgcggcct cgaaggccag cagatccgca
     4261 atcggcggca gaaatgtctt gcgcaagatg gtcatgcagt taaggaatga ttgaaacaca
     4321 aggatgacac ggccactacg gtgccagcgc actgtttacg ccgccaacac acaccgatac
     4381 ccgagataac cccaggtagt tcataccaaa ttttcatgaa atagttccaa tttatcgcta
     4441 tgcacaggcc cgtcaagcat ggatagttgc ggcgtcgcaa agccaatacg attcttcaag
     4501 gagacacaac atggcaagct cgtacaccaa gatggctgca accgccgccg gcctggcgct
     4561 gtggtgcgcc gccctgcccg cggcgctggc ccaggcaccg tcggccacgc tggacaagat
     4621 caaggcctcg ggcaaggcag tgctgggggt gcgcgaatcg tcgccaccta tggcctacgc
     4681 actgggcgcc aacgaaaaat acgtgggcta ccacgtcgag ctgtgcgaac gcgtgctcaa
     4741 ggagatcgcg cccacggcca agcttgaata catggccatc accgcacaaa gcacaatgcc
     4801 cctggtgagc aacggcacgg tagacattgg ctgcggcccc accaccaaca acctggcgcg
     4861 ccagcagcag gtggccttcg ccgtcaccag ctacgtgagc gaggtgcgca tggcagtgcg
     4921 caaggactct gacctcactt ccattgccca gctgggcggg cgcaccgtgg cggcctccac
     4981 cggtaccacg gccgtgcagc tgctgcgcaa gcaggagcgc gtggtgggct cgcccatcaa
     5041 gaccgtgctc ggcaaggatc accacgagag cttcatgctg cttgaatccg gccgggccga
     5101 tgccttcgtg ctggatgaca acatcctggc cggcgtcatc gccaactcca aggatccaag
     5161 cgcctaccgc atcgtgggcg agcccctggg tgccgagccc attgccctgc tgtttcgcaa
     5221 ggacgacccg ggcttcaagg ccgccgtgga tggtgtgctc acgcgcctga tgcaatccgg
     5281 cgagatggaa aaaatctata ccaagtggtt tgtgaacccc atcccgccca agaacgccag
     5341 cctgaacctg cccctgggca acgccctgcg ccagctgttt gccaccccga acgacaagcc
     5401 gctggaaacc taccaacagt aaagcccaca atgtctcctg ccctcacccc tcgcaccggc
     5461 ggccagatcc tggtgcgcca gctcatcacc catggtgtcc agcagctgtt ctgcgtaccc
     5521 ggtgagagct acctggccgt tctcgacgct ctgcatgacg ccgacatcgc cgtcaccgtc
     5581 tgccgccagg agggcggcgc cgcgatgatg gctgaggccc agggcaagct cacgggccgg
     5641 cccggcatct gcttcgtgac acgcgggccg ggagcgacca atgcctcggc cggcgtgcac
     5701 atcgcccacc aggactccac gcccatgatc ctgttcgtgg gccaggtggc gcgcggcgcc
     5761 aaggggcgcg aggccttcca ggagctcgat taccgcgccg tctttggcac catggccaag
     5821 tgggtggtgg acatcgacga cccggcgcgc ctgcccgagc tgatctcacg cgccttccac
     5881 gtcgccacct cgggccgccc cggcccggtg gtcgtggccc ttcccgagga catgctgacc
     5941 gaggccgcca gcgtggccga cgccctgccc taccaagtca cggaaacctg ccccggcacc
     6001 gccgcgctgc aagagctgca tgcacgcctg cagggggcgg aacaacccgt cgtcatcctc
     6061 ggcggcagcc gttggtccga ccaagccgta cgcgacatcg ccacatttgc cgagcactgg
     6121 caactgccgg tgtactgctc gttccgccgg cagatgctct tccccgccaa ccacgccagc
     6181 tacgccggcg acctgggtct gggcgtcaac cccaagctgc tggcacgcat gcgcgcgagc
     6241 gatctcgtgc tggtgctcgg tgcccggctg tcggaaatcc cttcgcaggg ctacgaactg
     6301 ttcgatatac ccaatcctgc acagccactg gtgcatgtac atgccgatgc cgacgaactt
     6361 ggccgcctgt accgcccggc gcagaccata catgccacgc cccaggcgat ggccgcggcg
     6421 ctggtcagcc tgcttccagc caccgcaccg ggttgggccg gacacacaca ggcggcgcgc
     6481 gccgaatatc tggcctggag cgaccccgcg cccatccgca tccccggcca gctgcaaatg
     6541 ggcgccgtga tgcagcacct gaagggcgta ctgcccgccg acacgatctt ctgcaatggc
     6601 gcgggaaatt tcgccacctg ggtgcaccgt ttctggccct tcaccagtta tggcagccag
     6661 ctcgcgccca ccagcggctc catgggctac ggcctgccgg caggtgtggg cgccaagcgc
     6721 ctatggcctg cacgcgaggt agtggtgttc gccggcgacg gcgactttct gatgcatggc
     6781 caggaattcg ccaccgccgt gcaatacggc ctgcccctgg tcgtggtgct gctggacaac
     6841 gccatgtacg gcaccatccg catgcaccag gagcgcgaat accccgggcg cgtcagcgcc
     6901 accgcgctca aaaaccccga cttcaaggcc tacgcccagg ccttcggcgg ccatggtgag
     6961 cgcgtcgaac gcaccgagga cttcgcccct gccctggagc gcgcccgcgc cagtggcctg
     7021 ccggcaatcc tgcactgctt catcgacccc gaggccatca ctccgaccgg cacgttgcaa
     7081 ggcatacgca acgcggcgct gaccaatggc tgacagcaag gcggccccaa gaaaacgagg
     7141 gtttttctgc tgatccggca aatctgcaag attccgccac aagcatggct ataatcgcgg
     7201 gcttcggaga ggtggatgag cggtttaagt cgcacgcctg gaaagcgtgt gtgggttaat
     7261 agcccaccgc gggttcgaat cccgccctct ccgccaaa
//
LOCUS       DAOMTQ010000144         7248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2357_length_7248_cov_26.571528, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000144 DAOMTQ010000000
VERSION     DAOMTQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_7248_cov_26.571528"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..451
                     /locus_tag="PK518_12435"
     CDS             254..451
                     /locus_tag="PK518_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HRM49765.1"
                     /translation="MNAAYSPLVSEFESAEQEASYTAWLQAKVAASLADPRPAIPHDE
                     VERRMAERLQRLRERAKREAS"
     gene            451..744
                     /locus_tag="PK518_12440"
     CDS             451..744
                     /locus_tag="PK518_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM49766.1"
                     /translation="MLPIFWLETADADLAAITDYIGARNINAAESMWHRLRSCVLPLS
                     EHPYLFPSSDRAPGLREIVAHPNYIVMYRVAAGRIEIVNVVHARQQFPRSPHE"
     gene            824..1996
                     /locus_tag="PK518_12445"
     CDS             824..1996
                     /locus_tag="PK518_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRM49767.1"
                     /translation="MSSSQDKQLPATERKLQKARSDGQAARSRDLSHLAILGVGALGL
                     LTLAPWFVEYLQRALRQQLVFDAATVQATGTMLQRLQTIAGVGVLASLAFALLTSGAV
                     LLAAVGAGGWIYSLKPITPQFNRLNPLSGFANLFSKQQLTNVSKMVLMTTILSFVAWR
                     YMSDSVEKMAQLVLQPSPLSLGHAGQWVVSGVALLLLVVFVFAVVDVPLQAFFFKQRL
                     KMSHEEVKQEHKESDGNPQLKGKQRQRAREIADRASITAVPKADFVVMNPTHYAVALK
                     YDEASMGAPQVIAKGTDLLAFRIRELAQQHSVPVLQSPMLARALYAHAELEQPIPAQL
                     YTAVAQVLAYVYRLKAALRGEGRMPDAQPDPYVPPELDPHHQPRHAGAALNAVDKT"
     gene            1993..4083
                     /gene="flhA"
                     /locus_tag="PK518_12450"
     CDS             1993..4083
                     /gene="flhA"
                     /locus_tag="PK518_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRM49768.1"
                     /translation="MTTPSPLHAMRQWAGTHGSALQGLAAPILVVAILALMVLPIPAW
                     LLDTFFTLNIAVALMVMMVAAYMLRPLDFAAFPSVLLLTTLMRLSLNVASTRVVLLEG
                     HTGPGAAGAVIEAFGHFLIGGNFAVGLIVFAILVVINFVVVTKGAERIAEVSARFTLD
                     AMPGKQMAVDADLNAGLIDEKEAKRRRAEVQEEANFFGSMDGASKFVRGDAVAGILIL
                     IINIVGGFAIGMLSHGLSAGDAANSYILLAVGDALVAQIPGLLISVAAAMVVSRVGKE
                     KDMGQQIVQQLFMSPRVLGVTAGVLILLGVIPGMPHAVFLVIGSGLAWGAWLLLQRQK
                     QAAVEPEAPPVPMGDGEASWDDLQPVDLLGLELGYRLIALVDKNRQGDLLTRIKGVRR
                     KFAQEVGFLPPPVHVRDNLELKPSGYRVLLRGVVVGEGEAFPGMFLAINPGGISTPLI
                     GTPTTDPAFGLPAHWIDAGHKEAAQMAGFTVVDPETVMATHLSHLMQVQAARLLSRTE
                     VQQLVEHVAKLAPKLIEEVVPKMVSITTFQKVLQLLLEESVHIRDIRTIIETLAEHAA
                     SVTDPAELARRVRIALAPAIVQQIYGPTRELAVIAIEPGLERLLVQALSNANGQALDP
                     GVADLLTHKAAEVALKQEELGMPACLLVPDVIRSPIARLVRRVAPRLQVLAHSEIPET
                     HTIRIGPILQGASS"
     gene            4080..5687
                     /gene="flhF"
                     /locus_tag="PK518_12455"
     CDS             4080..5687
                     /gene="flhF"
                     /locus_tag="PK518_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRM49769.1"
                     /translation="MNIKRFTAPTAREALAKARMAFGDGTLILSNRPTAEGVEVMATA
                     EETLSSSLQGDADAEPGSRLQQRADDLAASKPQRRLKDQPRPAATPVAAPARTSVAKD
                     TEQLAMSTLSFQDYVRERMLRRRHEALQGTGSATPLTERAALERGLPETQFERPLNPA
                     PNRAFEVHAEAAPQAPAAQPAAMPVVRHNPLRTMHAALGAEQLTPRRTEAAPTLAAGA
                     GQQQLMQELQSMRNLIEDRFNTLTWLGQARQNPIQTQLMLKLIRAGYSPALSRAVLEH
                     LPEQIEAPEAVRWLMEVLERNLRTDAQTQPLYEQGGIYAMVGATGVGKTTTAAKLAAH
                     CARIHGPGSVGLITLDTYRVGAHEQLRSYGRMLGVVAHLAHDRAALQDLLGLLAGKKM
                     VLIDTTGVAPRDPRKRELLDVLDLPEVQRLLVLNASSHGDTLDDVLTAFKTSGAQQAI
                     LSKVDEAVKLGPALDALIRHQLELRGVTNGQRVPEDWEAADAHKLIATSMRSKVSSAF
                     DPKVGELNFFFSPATEAAQNQGLAHVA"
     gene            5677..6477
                     /locus_tag="PK518_12460"
     CDS             5677..6477
                     /locus_tag="PK518_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49770.1"
                     /translation="MLLDTAIHQAMGLNSITPQRELRLLPVVSQPDGGPWLEMLWQLC
                     WHLQRLGYPVVVLDATAQESHRSPGLAQLLGARPWPGAEALNDASDDGSLAVLPAARG
                     LAWLAGQAELGQAGALQRLQPLLRAYAAVVLYAPPEQLAPLLHGSTSVPLVMTGPGAQ
                     GMVRSYRQLKHMALHAGVRCTVAAIVAPDAQAQPRQTAEALATLARSARQHLGQHIHT
                     TSVAAARAQDIQRLALQLLENAATLDPATPLLQPPGACATATHPDWSH"
     gene            6482..7198
                     /locus_tag="PK518_12465"
     CDS             6482..7198
                     /locus_tag="PK518_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HRM49771.1"
                     /translation="MYTAKGQLNRDAMFHQHMPLVRRIAHHMIAKLPPNVELDDLIQV
                     GLIGLTDALTRYEVTQGVQFETFASQRIRGAMLDELRGGDWMSRGARKSQKDIEQAMH
                     RVEQRLGRSPLESEIAAELGMQLDEYQSLLGKVRGTQLVYLEDMGGSDEGEEGFLDRH
                     AADEDADPMAVLRDQRLRAALVAAIKLLPEREQHIMGMYYEHDMNLKEIAAVLGVTES
                     RVCQLHSQAIARLRTKMRGH"
BASE COUNT         1216 a         2564 c         2380 g         1088 t
ORIGIN      
        1 ctagtttctc cctctaccat cgcgggctat cgtatgcaca catctttcga tagcgcgcaa
       61 cgcatatttg gcgccggttt taactccctc gcccccttgg gtgagaggga gaaaccgccc
      121 caaggccacg cacatgttgt gtggcacgct atacaaaagg tgtgtggata cccatgtcca
      181 gacggggaat gcgtccgagt cagctcgcga ctcaagttgg cctatatgcc ggggctggaa
      241 tagactgtgc cccatgaacg ccgcttattc ccctctggtt tcggaattcg agagcgccga
      301 gcaggaggcc agctacacgg cctggctgca ggccaaggtc gccgccagcc tggccgaccc
      361 gcggcccgcg atcccgcatg atgaagtaga gcgccgcatg gctgagcgcc tgcaacgcct
      421 gcgcgagcgc gccaagcgcg aagcgtcctg atgctgccca ttttctggct ggagacggca
      481 gacgccgatc tggcagccat caccgactac atcggggcac gcaacatcaa cgccgcagaa
      541 agcatgtggc acaggctgcg ctcctgcgta ttaccgctat cggaacaccc ctacctgttc
      601 ccctccagcg atcgcgcccc gggactgcgc gaaatcgtcg cccaccccaa ctacatcgtc
      661 atgtaccgcg tcgcggccgg gcgcatagaa atcgtcaacg tcgtgcatgc gcgccagcaa
      721 ttcccgcgca gccctcacga atagcgccgc cccggcccct ttattcgggc tttagcttct
      781 gcccctgctc ccgacaatgg cgtgacccag cagtcacgcc accatgagct ccagccaaga
      841 caaacaactc cccgcaaccg agcgcaagct gcagaaggcg cgcagcgacg gccaggccgc
      901 gcgctcgcgg gatttgtcgc acctggccat cctgggcgtg ggcgcgctgg ggctgctgac
      961 gctggcgccc tggttcgtgg aatacctgca gcgcgcgctg cgccagcagc tggtgtttga
     1021 cgccgccacg gtgcaggcca ccggcaccat gctgcagcgg ctgcagacca ttgccggcgt
     1081 gggcgtgctg gccagcctgg cgtttgcgct gctgaccagc ggcgccgtgc tgctggccgc
     1141 cgtgggcgcg ggcgggtgga tctacagcct caagccgatc acgccgcagt tcaaccggtt
     1201 gaatcctctg tcgggctttg ccaacctgtt ttccaagcag cagctgacca acgtctcgaa
     1261 gatggtgctg atgacgacca tcctgagctt tgtcgcctgg cgctacatga gcgacagcgt
     1321 ggagaagatg gcgcagctgg tgctgcagcc ctcgccgctg tcgctgggcc atgcgggaca
     1381 gtgggtcgtc tcgggcgtgg ccttgctgct gctggtggtg tttgtgtttg ccgtggtcga
     1441 tgtgccgctg caggccttct tcttcaagca acgcctgaag atgagccatg aggaggtcaa
     1501 gcaggagcac aaggagtccg acggcaaccc gcagctcaag ggcaagcagc gccagcgggc
     1561 gcgcgagatt gccgaccgcg ccagcatcac ggcggtgccc aaggccgact tcgtggtcat
     1621 gaaccccacg cactacgccg tggcgctgaa gtacgacgag gccagcatgg gcgcacccca
     1681 ggtgatcgcc aagggcacgg acttgctggc cttcagaatt cgtgagctgg cccagcagca
     1741 cagcgtgccg gtgctgcagt cgcccatgct ggcgcgtgcc ctctatgccc acgccgagct
     1801 ggagcagccc atccccgccc agctgtacac ggccgtggcc caggtgctgg cctatgtcta
     1861 ccgcctgaag gcggcgttgc gcggcgaagg ccgcatgccg gacgcgcagc ccgaccccta
     1921 tgtgccgccc gagctcgacc cgcaccacca gccccggcac gccggcgcgg ccctgaacgc
     1981 ggtggataag acatgaccac gccctccccg ctccacgcca tgcggcaatg ggccggcacc
     2041 catggctcgg ccctgcaggg cctggcggcg cccatcctgg tggtagccat attggcactg
     2101 atggtgctgc ccattccggc ctggctgttg gacacctttt tcacgctgaa catcgccgtg
     2161 gcgctgatgg tgatgatggt cgcggcctac atgctgcggc cgctggactt tgccgccttc
     2221 ccgtcggtgc tgctgctcac cacgctgatg cgcctgtcgc tgaacgtcgc ctccacgcgc
     2281 gtggtgctgc tggagggcca taccggcccg ggcgcggccg gcgcggtgat cgaggcgttt
     2341 gggcatttcc tgatcggcgg caactttgcc gtcggcctga tcgtgtttgc catcctggtg
     2401 gtcatcaact tcgtcgtcgt caccaagggc gcggagcgca tcgccgaggt gtcggcgcgc
     2461 ttcacgctgg acgccatgcc cggcaagcag atggcggtgg acgccgatct gaacgccggc
     2521 ctgatcgacg aaaaggaggc caagcgccgg cgcgccgagg tgcaggagga ggccaacttc
     2581 ttcggctcca tggacggcgc gagcaagttc gtacgcggcg acgcggtggc cggcatcctg
     2641 atcctgatca tcaacatcgt cggcggcttt gccatcggca tgctctcgca cggcctgtcg
     2701 gccggcgacg cggccaacag ctacatcctg ctggccgtgg gcgatgcgct ggtggcgcag
     2761 attccgggcc tgctcatctc ggtggccgcg gccatggtgg tctcgcgcgt gggcaaggaa
     2821 aaggacatgg gccagcagat cgtgcagcag ctgttcatgt cgccgcgcgt gctgggcgtg
     2881 acggccggcg tgctgatcct gctgggcgtg ataccgggca tgccgcatgc tgtgttcctg
     2941 gtgatcggca gcggcctggc ctggggtgcc tggctgctgc tgcagcgcca gaagcaggcc
     3001 gccgtcgagc ccgaggcacc acccgtcccc atgggcgacg gcgaggccag ctgggacgac
     3061 ctgcaacccg tggatctgct gggcctggag ctgggctacc gcctgatcgc cctggtggac
     3121 aagaaccgcc agggcgacct gctcacgcgc atcaagggcg tgcggcgcaa gtttgcccaa
     3181 gaggtgggtt ttctgccgcc gccggtgcat gtgcgcgaca acctcgaact gaaacccagc
     3241 ggctaccgcg tgctgctgcg cggcgtggtg gtgggcgagg gcgaggcctt ccccggcatg
     3301 ttcctggcca tcaaccccgg cggcatctcc acgccgctga ttggcacacc caccaccgac
     3361 ccggccttcg gcctgccggc gcactggatc gatgccgggc acaaggaagc ggcacaaatg
     3421 gcgggtttta cggtggttga tccggaaacc gtgatggcca cccatttgtc acacttgatg
     3481 caagtacagg ccgccaggct cctgagtcgc accgaggtgc agcagctcgt ggagcatgtg
     3541 gccaagctgg cccccaagct catagaagaa gtggttccca agatggtctc gatcacgacg
     3601 ttccagaaag tgctgcagct cttgctggaa gagtccgtgc acatccgcga catccgcacc
     3661 atcatcgaaa ccctggccga gcatgccgcc agcgtcaccg accccgccga gctggcacgg
     3721 cgcgtgcgca ttgcgctggc gccggccatc gtgcagcaga tctacgggcc gacgcgcgag
     3781 ctcgccgtga tcgccatcga gccgggcctg gagcgcctgc tggtgcaggc cctctccaat
     3841 gccaatggac aggcgctgga tccgggcgtg gccgacctgc tgacacacaa ggcggccgag
     3901 gtggcgctca agcaggagga actgggcatg cccgcctgcc tgctggtgcc cgacgtgatt
     3961 cgcagcccca tcgcccgcct ggtgcgccgc gtggcgccgc gcctgcaggt gctggcgcac
     4021 agcgagattc ctgaaaccca caccatccgc atcggcccca tcctccaagg tgcatcgtca
     4081 tgaacatcaa acgctttacc gcccccaccg cccgcgaggc cctggccaag gcgcgcatgg
     4141 ccttcggcga cggcacgctg atcctgtcca accgccccac ggccgagggc gtggaggtga
     4201 tggccacggc cgaggaaacc ctgtcgtcca gcctgcaggg cgacgccgat gcagagcccg
     4261 gcagccgcct gcagcagcgt gccgacgacc tggccgcaag caagccgcaa cgccggctga
     4321 aagaccagcc ccgccccgcc gccacgcccg tcgccgcacc ggcgcgcacc agcgtggcca
     4381 aggacaccga gcagctggcc atgagcacgc tgtcgttcca ggactatgtg cgcgagcgca
     4441 tgctgcgccg tcgccacgag gcgctgcagg gcacgggcag tgccacgccc ttgactgagc
     4501 gcgccgcgct ggagcgcggc ctgcccgaga cacagttcga gcgcccgctg aaccccgcgc
     4561 cgaaccgcgc atttgaagtc cacgccgaag ccgcgccgca ggcgccagcc gcccaacccg
     4621 cggccatgcc cgtggtgcgc cacaacccgc tgcgcaccat gcatgccgcc cttggcgccg
     4681 agcagctcac gccgcgccgc accgaggccg cccccacgct ggcggccggc gccgggcagc
     4741 agcagctgat gcaggagctg cagtccatgc gcaacctgat cgaagatcgc ttcaacaccc
     4801 tgacctggct cggccaggca cggcaaaacc cgatacagac ccagctcatg ctcaagctga
     4861 tccgcgcagg ctactcgccg gcgctgtcgc gcgcggtgct ggagcatctg cccgagcaga
     4921 tcgaggcccc ggaggccgtg cgctggctca tggaggtgct ggagcgcaac ctgcgcaccg
     4981 acgcgcagac acagccgctg tacgagcagg gcggcatcta cgccatggtc ggcgccaccg
     5041 gcgtgggcaa gaccaccacc gcggccaagc tggcggcgca ctgcgcacgc attcacggcc
     5101 cgggcagcgt cggcctgatc acgctggaca cctaccgcgt gggtgcgcac gagcagctgc
     5161 gcagctacgg ccgcatgctg ggcgtggtgg cgcacctggc gcatgaccgc gccgcgctgc
     5221 aggacctgct gggcctgctg gcgggcaaga agatggtgct gatcgacacc accggcgtgg
     5281 cgccgcgcga cccgcgcaag cgcgaactgc tggacgtgct ggatctgccc gaggtgcagc
     5341 gcctgctggt gctcaacgcc agcagccatg gcgacacgct ggacgacgtg ctgacggcct
     5401 tcaagaccag cggcgcgcag caggccatcc tgtccaaggt ggacgaggcc gtgaagctcg
     5461 gcccggcgct ggacgcgctg atccgccacc agctggaact gcgcggcgtg accaacggcc
     5521 agcgcgtgcc cgaggactgg gaggcggccg acgcgcacaa gctgatcgcc acctcgatgc
     5581 gcagcaaggt cagctcggcc ttcgacccca aggtgggcga gctcaacttc ttcttctcgc
     5641 ccgccaccga agcggcccag aaccaaggac tggcccatgt tgcttgacac cgccatccac
     5701 caggccatgg gcctgaacag catcacaccg cagcgcgaac tgcgcctgtt gcccgtggtc
     5761 agccagcccg atggtggccc gtggctggag atgctgtggc agctgtgctg gcacctgcag
     5821 cgcctgggct atcccgtggt ggtgctggac gcaacggccc aggaaagcca ccgcagcccg
     5881 ggccttgcgc aactgctggg cgcccgcccc tggcccggtg ccgaggcatt gaacgacgcg
     5941 agcgacgacg gctccctggc cgtgctgccc gccgcacgcg gcctggcctg gcttgccggg
     6001 caggcagagc tgggccaggc cggcgccctg cagcgcctgc agccactgct gcgcgcctat
     6061 gccgccgtgg tgctgtacgc gccacccgag cagctggcac cgctgctgca cggcagcacc
     6121 agcgtgcccc tggtcatgac cggcccgggc gcgcagggca tggtgcgcag ctatcgccag
     6181 ctcaagcata tggcgctgca tgccggcgtg cgctgcacgg tcgccgccat cgtggcgcct
     6241 gacgcccagg cgcagccgcg ccagaccgcc gaggccctgg ccacgctggc gcgcagtgcc
     6301 cgccagcacc tggggcagca tatccacacc accagcgtgg ctgcggcgcg cgcccaggac
     6361 atccagcgcc tggcgctgca gctgctggaa aatgccgcca ccctggaccc cgccacgccc
     6421 ctgctgcagc cgcccggcgc ctgcgccacg gccacgcacc cggactggag ccactgagac
     6481 gatgtacacc gccaaaggcc agctcaaccg cgacgcgatg tttcaccagc atatgccgct
     6541 ggtgcgtcgc attgcccacc acatgatcgc caagctgcca cccaacgtgg agctggacga
     6601 cctgatacag gtgggcctga tcggcctgac cgacgcgctc acacgctacg aggtcacgca
     6661 gggcgtgcag ttcgagacct ttgccagcca gcgcatccgc ggcgccatgc tcgacgagct
     6721 gcgcggcggc gactggatga gccgcggcgc gcgcaagagc cagaaggaca tcgagcaggc
     6781 catgcaccgc gtcgagcagc gcctggggcg cagccccctg gaatccgaga tcgccgccga
     6841 gctgggcatg cagctggacg agtaccagtc gctgctgggc aaggtgcgcg gcacgcagct
     6901 ggtgtacctg gaggacatgg gcggcagcga cgagggcgag gaaggcttcc tcgaccgcca
     6961 cgcggccgac gaggacgccg accccatggc cgtgctgcgc gaccagcgcc tgcgcgccgc
     7021 cctggttgcc gccatcaagc tgctgcccga acgcgagcag cacatcatgg gcatgtacta
     7081 cgagcacgac atgaacctga aggaaatcgc cgccgtcctg ggcgtcaccg agtcgcgcgt
     7141 ctgccagctg cacagccagg ccatcgcccg cctgcgcacc aagatgcgcg ggcactgaaa
     7201 gcctcattgg ggtcagattc caattaattg gttaaatatg gatctgac
//
LOCUS       DAOMTQ010000145         7227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2371_length_7227_cov_25.695761, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000145 DAOMTQ010000000
VERSION     DAOMTQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_7227_cov_25.695761"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..875)
                     /locus_tag="PK518_12470"
     CDS             complement(69..875)
                     /locus_tag="PK518_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM49772.1"
                     /translation="MTSIVFSHANSFPAGTYRVLFDLLRQRGFTVSAVERFGHDARYP
                     VSNNWPHLVQQLADFAAAQQQRTGEPAFLVGHSLGGFLSVMAAARYPQLARGVLLIDS
                     PLIGGWRASALGVAKQAQVVGAVTPGRISRARRNSWASTEEALEHFRRKRVFAQWDPQ
                     VLQDYVTHGLQERDGQRVLVFDREVETRIYNTLPHNLGRILQAHPLQCPAAFIGGRDS
                     AEMRQVGMAMTQRITQGRVMMLDGSHLFPMEQPQATAAAIEAALLNLQNI"
     gene            1065..2174
                     /locus_tag="PK518_12475"
                     /pseudo
     CDS             1065..2174
                     /locus_tag="PK518_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            2253..5453
                     /locus_tag="PK518_12480"
     CDS             2253..5453
                     /locus_tag="PK518_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM49773.1"
                     /translation="MNLSRLFILRPIATSLLMVAILIAGLLAWRQLPISALPEVDYPS
                     IQVTTLYPGASPDVMTSNVTAPLERQLGQMPGLAQMSSTSSGGASVITLRFALDMSMD
                     VAEQQVQAAINAATSLLPNDLPMPPLYSKVNPADAPILTLAITSPTLPVIRVNDLVEN
                     RLAPRLSQVQGVGLVAIGGGRRPAVRIQANPTALAGLGLTLEDLRTSIAAANVKAAKG
                     GFDGPERASAIDANDQLQSAAEYRQLIIAFKNGNPVRLADVAATVDDAENTRLAAWAA
                     TPEAGARAGVILNIKRQPGANVIATVDRIQALLPQLKESLPASLDVQVLTDRTVTIRA
                     SVRDMQTELMLAIALVVAVIFVFLRSASATLIPSFAVPLSLVGTFGVMHLAGFSINNL
                     TLMALTISTGFVVDDAIVMIENIARHLEMPGNVSVQAHGAEGPVKDAQASGHSQNTPA
                     PHITPLEAALQGAKQIGFTIISLTISLVAVLIPLLFMGDVVGRLFREFAITMAVAILI
                     SAVVSLTLTPMLCARLLRHDPEQARGRLYHATGRFLDGMIAGYGRLLAWVLDRRGLTW
                     GVFLATLAITALLYLAVPKGFFPTQDTGTLQATTEADQSISFAAMAERQQALVERLLR
                     DPAVQSISSFIGVDGSNTTLNTGRLLIDLKPHGERDDAATVLRRLSDDSRGLPGIRLF
                     AQPVQDLTIEERAARTQYQLLLSSPDMAQLARSSAALVERMRALPELTDVGSDQQNLG
                     RQAYVEIDRAQASRLGVTVAAVDQALYNAFGQRLISTIFTQSNQYRVVLEVAPQFKLG
                     PEALQAIHVASTSGAPVPLSSIAQVSERSMALAVSHVGQLPAATISFNTAPGVALGHA
                     VKAVQGQLQAARDAGELPLAVEAQFQGAALAFQASLSNTLLLILAAVITMYIVLGVLY
                     ESTIHPVTILSTLPSAAVGALLALMVAGLELDIIAIIGIVLLIGIVKKNAIMMIDFAL
                     EAQREQGLSAREAIFQACLLRFRPILMTTLAALLSALPLMLGQGVGSELRHPLGVTMV
                     GGLLLSQLLTLFTTPVIYLTFEGWAQRWRGSEVKNA"
     gene            5715..>7227
                     /locus_tag="PK518_12485"
     CDS             5715..>7227
                     /locus_tag="PK518_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM49774.1"
                     /translation="MSLSTPFIHRPIATMLLTLGLALAGAVSYFLLPVAPLPQVDYPT
                     ISVSASLPGASPDTMAATVATPLERSLGAIAGVNEITSSSSLGNTRITLQFDLSRTVD
                     SAAHDVQAAINAARTLLPTGMPSSPTYRKVNPADAPIMILALTSASLTRGQMYDAAST
                     VLAQKLSQVEGVGQAVVSGGALPAVRVELDPVRLAASGISLEQVRGAITSTNANRPLG
                     ALERGGQYWQVATNDQARAAADYAPLVLAWRNGGAVRLADVARVTDSVQDVRNYGVAN
                     GQPAILLQIYKQPGANILDAVERVRALLPQLQASIPAAIDITVVSDRTPTLRASVDEV
                     QRALLIAVALVILVVFLFLRRLRATLIPAVAVPASLAGTFGVMYLAGYTLDNLSLMAL
                     TVATGFVVDDAIVVLENVMRHMERGKTALQAALDGAREIGFTVVSMSLSLIAVFVPIL
                     FMGGIVGRFFREFAVVMSAAILLSMLVSLTTTPMMCAALLRAPAPGRAAPGAWA"
BASE COUNT         1091 a         2460 c         2489 g         1187 t
ORIGIN      
        1 gggcggaatg agctattgaa aatatagctt ttggcgcaat atggataagc gctagcggca
       61 aattttgctt agatattttg taggttcagc agcgccgcct cgatggccgc cgccgtggcc
      121 tggggctgct ccatgggaaa gaggtggctg ccgtcgagca tcatcacgcg gccctgggtg
      181 atgcgctgcg tcatggccat gcccacctgg cgcatctctg ccgagtcgcg cccgcctatg
      241 aaggcggccg ggcattgcag cgggtgggcc tgcaagatgc gccccaggtt gtgcggcagg
      301 gtgttgtaga tccgggtttc cacctcgcgg tcaaagacca gcacgcgctg gccgtcgcgc
      361 tcctgcaggc catgggtcac gtagtcctgc agcacctgcg ggtcccattg ggcaaagacc
      421 cgcttgcgcc ggaaatgctc cagcgcctcc tcggtgctgg cccagctgtt gcgccgcgca
      481 cggctgatgc gcccgggcgt gacggcgccc accacctggg cctgcttggc cacacccagg
      541 gcgctggcgc gccagccgcc gatgagtggc gagtcgatga gcagcacgcc gcgcgccagt
      601 tgcgggtagc gcgcggcggc catcacgctc aagaagcccc ccagggagtg gcccaccaga
      661 aaggcgggct ctcccgtgcg ctgctgctgc gcggcggcaa agtcggccag ttgctgcacc
      721 aggtgcggcc agttgttgct gacgggatag cgcgcgtcgt ggccaaagcg ctccacggcg
      781 ctgaccgtga agccgcgctg gcgcagcaga tcgaacagca ctcggtaggt gccggccggg
      841 aagctgttgg cgtgggagaa gacgatgctg gtcatggtgc gggtgggttg ccccgagtgt
      901 agtggtgccg ttgcagtgtt tgcgcccgct gcatgcgcca ccacggcgcg gcccgctatg
      961 atcttttgct ctttgcagga gaaaccccct tgcccgcccc ccagtgtcgc cacaggtatc
     1021 gccgccctca tgaacaccga tgcgctgggc gtatccggcc ggtgctgctg accatcttgc
     1081 tgctggcctt gctggcggcg gccggcggct ggtggtatgt gaagagccag ggcgggcagg
     1141 gcgacggccc gggcgcgtcg ggccgccctg gcgggccgtc agcgcagccg gtgtcggtgg
     1201 gtgtggtgga caggcgcgac atgcgcgtgc tggtcagcag cattggcacc atgaacgccc
     1261 gcgccaccac cgtggtgcgc acccgcgtgt cgggcgagct ggagaagatt tttttcaagg
     1321 agggcgacga ggtgcgcgcc ggccagctgc tggcgcagat cgacgcgcgc ggctaccgcg
     1381 ccgcgctgca gcaggcccag ggtacgctgc agcgcgacca ggcgctgctg aagaacgccc
     1441 agctggacct gcggcgctac caggagctgc aggcgcaaga ttccatcgcc agccagcagg
     1501 tggacaccca ggcggccctg gtgcgccagt accagggcac ggtggccgcc gaccaggcgc
     1561 aggtggatgc agcgcggctg cagcttgatt acacgcgcat caccgcgccg attgccgggc
     1621 gcctgggcct gcgccaggcg gatctgggca atgtgctgaa cccctcggac gtgagtggca
     1681 tcgtcaccat cacccaggtg cggccgatag acgcgctgtt ctcagtgccc gaggcgcatg
     1741 tcggcgccat cgcccgcgcc ctggccgaga agcgcgcgct gcccgtggag ctgtgggacc
     1801 gcgagcaaaa gagcctgctg gccaccgggc gcctgaatgc gctggacaac gccatcgacg
     1861 ccagcaccgg caccgtcagg gccaaggcgg cgttcgacaa cgccgatggc cggctgtttg
     1921 ccaaccagtt cgtcaacgtg cggctgcaga tccggctcat cgagcaggcg cttaccgtgc
     1981 cggccacggc cgtgcagaac aaccatgtct acctgttgca ggccgacggc caggtgacgc
     2041 agcgccgcct ggagctgggc gtgactgacg gcgatcgcgt cagcgtcacc ggcgatctgc
     2101 agcccggcga ccgggtggtc accgacggcc tggaccgcct gcgcgagggc agcaaggtaa
     2161 cggtgataga ccccggccag gcgcaggcgc cggccgccga taggccgcgg gcggacaagg
     2221 gccgcgcgcg ctgaaacccg cagcgccctg ccatgaacct gtcgcgcctg ttcatcctgc
     2281 ggccgatcgc cacctcgctg ctgatggtgg ccatcctgat cgccggcctg ctggcctggc
     2341 gccagctgcc gatatcggcc ctgcccgagg tggactaccc cagcatccag gtgaccacgc
     2401 tctaccccgg ggccagcccg gacgtgatga cctccaacgt caccgcgccg ctggagcgcc
     2461 agctcggcca gatgccgggc ctggcgcaga tgtcgtccac cagctcgggc ggggcgtcgg
     2521 tgatcacgct gcgctttgcg ctcgatatgt cgatggacgt ggccgagcag caggtgcagg
     2581 ccgccatcaa cgccgccacg tcgctgctgc ccaatgacct gccgatgccg cccttgtata
     2641 gcaaggtgaa cccggccgac gcgcccatcc tgacgctggc catcacctcg cccacgctgc
     2701 cggtgatacg cgtgaacgac ctggtggaaa accgcctggc gcccaggctt tcgcaggttc
     2761 agggcgtggg cctggtggcg attggcggcg ggcgccgccc ggccgtgcgc atacaggcca
     2821 accccacggc gctggccggc ctggggctga cgctggagga cttgcgcacc tccatcgccg
     2881 ccgccaacgt caaggccgcc aagggcggct tcgacgggcc cgagcgcgcc tccgccatag
     2941 atgccaacga ccagctgcag tccgccgccg agtaccgcca gctgatcatc gccttcaaga
     3001 acggcaaccc ggtgcgcctg gccgacgtgg ccgccaccgt ggacgacgcc gagaacaccc
     3061 gcctggccgc ctgggccgcc acgcccgagg cgggcgcgcg cgccggcgtc atcctgaaca
     3121 tcaagcgcca gcccggcgcc aacgtcatcg ccacggtgga ccgcatccag gccctgctgc
     3181 cgcagctgaa ggagtcgctg cccgcgtcgc tggacgtgca ggtgctgacc gaccgcaccg
     3241 tgaccatacg cgcctcggtg cgcgacatgc agaccgagct gatgctggcc atcgccctgg
     3301 tggtggcggt gatcttcgtc tttctgcgct cggcatcggc cacgctgatc cccagctttg
     3361 ccgtgccgct gtcgctggtg ggcacctttg gcgtgatgca tctggcgggg ttctcgatca
     3421 acaacctgac gctgatggcg ctgaccatct ccaccggctt tgtggtcgat gacgcgattg
     3481 tcatgatcga aaacattgcc cggcatctgg agatgccggg caatgtttcg gtgcaggctc
     3541 atggggccga aggccccgtg aaggacgcgc aagcgtccgg ccatagccag aacacgccag
     3601 caccccacat cacccccttg gaggctgcac tccagggcgc caaacagata ggcttcacca
     3661 tcatctcgct gaccatctcg ctggtggcgg tgctgattcc gctgctcttc atgggcgacg
     3721 tggtggggcg gctgttccgg gagttcgcca tcaccatggc cgtggccata ctgatatcgg
     3781 ccgtggtctc gttgacgctc acgcccatgc tgtgcgcgcg cctgctcagg catgacccgg
     3841 agcaggcccg tggccgcctg taccacgcca ccgggcgttt tctggacggc atgatcgccg
     3901 ggtacggccg gctgctggct tgggtgctgg accgtcgcgg cctgacctgg ggcgtgtttc
     3961 tggccacgct ggccatcacc gcgctgctgt acctggcggt gcccaagggc ttcttcccga
     4021 cgcaggacac gggcacgctg caggccacca ccgaggccga ccagtcgatc tcgttcgccg
     4081 ccatggccga acgccagcag gcactggtcg agcgcctgct gcgcgacccg gcggtgcaga
     4141 gcatttcctc cttcatcggc gtggatggca gcaacaccac gctgaacacc ggccgcctgc
     4201 tcatcgacct gaagccccac ggcgagcgcg acgacgccgc caccgtgctg cgccgcctgt
     4261 cggacgacag ccgcggcctg cccggcatac ggctgtttgc ccagccggtg caggatctga
     4321 cgatagagga gcgcgcggcg cgcacccagt accagctgct gctgtcatcg cccgacatgg
     4381 cgcagctggc gcgcagcagc gcggccctgg tcgagcgcat gcgcgccctg cccgagctga
     4441 ccgacgtggg cagcgaccag cagaacctgg gccgccaggc ctatgtggag atagaccgcg
     4501 cccaggccag ccgccttggc gtgacggtgg cggcggtgga ccaggccctg tacaacgcct
     4561 tcggccagcg gctgatctcg accatcttca cgcagtcgaa ccaataccgc gtggtgctgg
     4621 aggtggcgcc gcagttcaag ctggggcccg aggcgctgca ggccatccat gtggcctcga
     4681 ccagtggcgc gccggtgccg ctgtcctcca tcgcccaggt cagcgagcgc agcatggccc
     4741 tggccgtcag ccatgtgggg caactgcccg cggccaccat ctccttcaac accgcgcccg
     4801 gcgtggccct gggccatgcc gtcaaggcgg tccagggcca actgcaggcc gcgcgggacg
     4861 ccggcgagct gccgctggcg gtggaggcgc agttccaggg cgcggcgctg gcgttccagg
     4921 cgtccttgtc caacacgctg ctgctgattc tggcggcggt gatcaccatg tacatcgtgc
     4981 tcggcgtgct gtatgagagc accatccacc cggtgaccat tttgtccacg ctgccctcgg
     5041 cggcagtggg ggcgctgctg gcgctgatgg tggcggggct ggagctggac atcatcgcca
     5101 tcatcggcat cgtgctgctg atcggcatcg tgaagaagaa cgccatcatg atgatcgact
     5161 tcgccctgga ggcgcagcgc gagcagggcc tgagcgcgcg cgaggccatc ttccaggcct
     5221 gcctgctgcg ctttcgcccc atcctgatga cgacgctggc ggcgctgctg agcgccctgc
     5281 cgctgatgct gggccagggc gtgggcagcg aactgcgcca cccgctgggt gtgaccatgg
     5341 tggggggcct gttgctgagc cagttgctga cgctgttcac cacgccggtg atctatctga
     5401 cctttgaggg ctgggcgcag cggtggcggg gttctgaggt gaaaaatgcc tgaagcgctt
     5461 gtgtaccaag cgctaggcgc tatctttatt tctaattggg gtcagattcc aattaaatcc
     5521 tggctggttg tttgcttgtt gctggccggg gcgagacccg gccagccata tcaaaaagaa
     5581 gagctttcag cgccctatca gcaagcgcta gatgccaaaa ttaattggag tctgacccca
     5641 attaggcttc caaacaggtg taagcgggta gttaattgga atctgacccc aattaagggc
     5701 gcggggggtg tggcatgagc ctgtccaccc ccttcatcca ccgccccatc gccaccatgc
     5761 tgctgaccct gggcctggcg ctggccgggg cggtgtcgta ttttttgctg ccggtggcgc
     5821 cgctgccgca ggtggattac ccgacgatct cggtcagcgc cagtctgccc ggcgccagcc
     5881 ccgacaccat ggccgccacg gtggcgacgc cgctggaacg ctcactgggc gcgatcgccg
     5941 gggtgaacga gatcacctcc agctccagcc tgggcaacac gcgcatcacg ctgcagttcg
     6001 acctgtcgcg caccgtggac agcgccgcgc acgacgtgca ggccgccatc aacgccgcgc
     6061 gcacgctgct gcccaccggc atgcccagca gcccgaccta ccgcaaggtg aacccggcgg
     6121 acgcgcccat catgatcctg gcgctcacct ccgccagcct cacgcgcggg cagatgtacg
     6181 acgcggcctc gacggtgctg gcgcaaaagc tctcgcaggt cgagggcgtg ggccaggcgg
     6241 tggtcagcgg cggggcgctg ccggcggtgc gtgtcgagct cgatccggtg cggctcgcgg
     6301 ccagcggcat ctcgctggag caggtgcgcg gcgccatcac cagcaccaac gccaaccggc
     6361 cgctgggcgc tctggagcgg ggcggccagt actggcaggt ggccaccaac gaccaggcgc
     6421 gcgcggcggc cgactacgcg ccgctggtgc tggcctggcg caatggcggc gccgtgcgcc
     6481 tggcggacgt ggcgcgcgtc accgactcgg tgcaggacgt gcgcaactac ggcgtggcca
     6541 acggccagcc ggccatattg ctgcagatct acaagcagcc gggcgccaac attttggacg
     6601 cggtggagcg cgtgcgcgcg ctgctgccgc agctgcaggc ctcgataccg gcggcgatcg
     6661 acatcaccgt ggtgtcggac cgcacgccca cgctgcgcgc ctcggtggac gaggtgcagc
     6721 gcgcgctgct gattgccgtg gcgctggtga tcctggtggt gttcttgttt ttgcgccggc
     6781 tgcgcgccac gctgattccg gccgtggccg tgccggcgtc gctggccggc acctttggcg
     6841 tgatgtacct ggcgggctat acgctggaca acctgtcgct gatggcgctg acggtggcca
     6901 ccggctttgt ggtggacgac gccatcgtgg tgctggagaa cgtcatgcgc cacatggagc
     6961 gcggcaagac ggccctgcag gcggcactcg acggcgcgcg cgagattggc tttaccgtgg
     7021 tctcgatgag cctgtcgctg atagcggtgt tcgtgcccat attgttcatg ggcggcatcg
     7081 tcgggcgctt ctttcgcgag ttcgcggtgg tcatgtccgc ggccattttg ctgtccatgc
     7141 tggtgtcgct gaccaccacg cccatgatgt gcgcggccct gctgcgcgcg cccgcgccgg
     7201 gccgggctgc gccgggcgcc tgggcac
//
LOCUS       DAOMTQ010000146         7227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2372_length_7227_cov_25.283882, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000146 DAOMTQ010000000
VERSION     DAOMTQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_7227_cov_25.283882"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PK518_12490"
     CDS             <1..1023
                     /locus_tag="PK518_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRM49775.1"
                     /translation="AAPAPAPRAREPGKPVKPRPRPAPAAPPAATEDSPADSPVQSAP
                     PAPIISPMEAEAAPASTEAAMPGSDSQAAPQVPPTTAEAPEPDKAAAAEPEKAAAAES
                     AETAGVEIRPPGQPGARASSEPPPVLLPPSTRLSFDVVGEVKKFHYNASAELLWRQQG
                     QRYEARQQIKAFLLGARTQTSVGSITPQGLQPERFGDKSRSERAAHFDFERHEVIFSA
                     NTPRATIGAGAQDRLSVFLQLGAMLAAAPERYPPGTQITLTTVGARHADRWTFSVQGP
                     ETLDLPAGSTPALKLERLPQADQQRDQKAELWLGTALHYLPVRIRLTQGNGDFADLLL
                     SGHEAP"
     gene            1171..1461
                     /locus_tag="PK518_12495"
     CDS             1171..1461
                     /locus_tag="PK518_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HRM49776.1"
                     /translation="MKMLYDSDSFVVVHMLPDAGLSDSAAKTAPLPPQLLRHGFEIVD
                     KRSGKEVYLDGSWAELFQQQILAWQRDTPTQEEVEDTLDSYVGLAQNPVVVH"
     gene            complement(1667..2221)
                     /locus_tag="PK518_12500"
     CDS             complement(1667..2221)
                     /locus_tag="PK518_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRM49777.1"
                     /translation="MATRVSLPGMRSPGAGFDQPFEMLEACHDRVRRSLDLLRRLRDY
                     LRGHGHGCDDSARQAARDVLRYFDIAAPLHHEDEELHVFPPLLAQEHDAPLVTLVRQL
                     QRDHVRMAERWATARGPLQAMADGQLPAFSAEHEAALDGFAECYADHLRHEDDSIYPA
                     ARALLDPGAQSAMGQEMARRRGVR"
     gene            complement(2228..2647)
                     /locus_tag="PK518_12505"
     CDS             complement(2228..2647)
                     /locus_tag="PK518_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HRM49778.1"
                     /translation="MKTEINHFDDLLHAARAQRERQRLLLVFAGTELPEDATPGQRER
                     FAQGDGGVLVPMMCLDRLPEELESFAALLRESRQFEPPGQPWRLVFTAALSGTPTRAP
                     SEDDAERVLGRMVEGVKTGAFDAYLPFNREGQPVRLG"
     gene            complement(2622..3278)
                     /locus_tag="PK518_12510"
     CDS             complement(2622..3278)
                     /locus_tag="PK518_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRM49779.1"
                     /translation="MGGASAADLAAALLQSRQTILPKRLGAPGPDAAQLQAMLAAAGH
                     APDHGRLLPWRFVLVPQEARAALAEVFAQALCERDSAATPEQQEQAREKAFRAPLLLL
                     LAVDELGGDPDIVPAERLVSAGCAVQNLLLMATALGFGSALTSGKALQSQGLRALFAL
                     GTGERALCFVSVGTVLARKPARVRPVPAQYVSTLVPGVGLQPWMDKGGHGHEDRNQPL
                     "
     gene            complement(3382..4215)
                     /locus_tag="PK518_12515"
     CDS             complement(3382..4215)
                     /locus_tag="PK518_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM49780.1"
                     /translation="MTSTCGTHACGCGSASASDSASDQAEVARINGVALHAAGAQPPG
                     DMLRQRACTELLRQQAQRQGLLAEDDAPGLDGATSAAAAQAIERLLEQALQVPEPSEA
                     ACRRYHAAHPGFGSQGERAHLRHVLFAVTPGVDVSLLRQRAERLLLELRCADDGGPRF
                     AEAAGEWSNCPSGAQGGDLGWLTPEDCAPEFAREVFGSQEVGVLARLVHSRFGLHVVQ
                     VCARKPAQPQAFDQVQASIALVLRQQAWVNALRQYLQLLAGSAEVEGVQLDAADSPLV
                     Q"
     gene            complement(4312..5040)
                     /gene="narI"
                     /locus_tag="PK518_12520"
     CDS             complement(4312..5040)
                     /gene="narI"
                     /locus_tag="PK518_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRM49781.1"
                     /translation="MTAWIDHFLFGLYPYICLAVFFIGSWLRFDRDQYTWKSDSSQLL
                     RTGSLRWASNLFHVGVLFLFFGHFVGMLTPHVLYDYVITAHDKQLLAMVSGGIAGLMA
                     FVGVTLLLHRRLTDARIRATSKTSDIALLWIFWVQLALGLATIPLSAQHLDGSMMMRL
                     AEWGQRIVTFRSGAVEMLAGAGWVFKAHLFLGMTVFLIFPFTRLVHVWSGFGTLAYVL
                     RPYQLVRARRLNVPPGQNEPRRSL"
     gene            complement(5037..5735)
                     /gene="narJ"
                     /locus_tag="PK518_12525"
     CDS             complement(5037..5735)
                     /gene="narJ"
                     /locus_tag="PK518_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRM49782.1"
                     /translation="MFKKTPETMRLTLRALARLLAYPGAGLRAQMPALIDALQTEQAL
                     PAARIAELQALAQQICAMDAYEAEGRYVDTFDRGHRTSLHLFEHIHGDSRERGPALID
                     LTQTYERAGLYLDAQELPDHLGVVLEFASTQPTAVAREFLAEMAHILNALFSALQAKA
                     SPYASVLAAVLEAAGETAQAVAIAPEPDMDEAWAEPEAFDGCATRGQNRPDQPQPIHI
                     VRKARPQSSQGVSP"
     gene            complement(5747..>7227)
                     /gene="narH"
                     /locus_tag="PK518_12530"
     CDS             complement(5747..>7227)
                     /gene="narH"
                     /locus_tag="PK518_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRM49783.1"
                     /translation="CIGCHTCSVTCKNVWTSRPGMEYAWFNNVETKPGIGYPKEWENQ
                     DKWNGGWTRHADGSITPRQGGKWKLLMRIFANPNLPQIDDYYEPFTFDYDHLQSAPEM
                     KNTPVARPRSLITGERLEKIVWGPNWEEILGGEFSRRSADVNFEQVQKDMYAQFENTF
                     MMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYY
                     NWQTGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIQEAASVERDKDL
                     YQAQLDIFLDPHDPEVIRQARLDGIPDNWLEAAKNSPVYKMAVDWKVALPLHPEYRTL
                     PMVWYVPPLSPITSAANAGHVGVNGEIPDVSALRIPVQYLANLLTAGDTGPVVRALER
                     MLAMRAYQRGVHVDKVQNMAVLQQTGLTVHDVEDMYQVMAIANYEDRFVIPSTHREYA
                     EDAFDVRGGCGFSFGNGCSDGATEVSIFGGKKPRTIPIKAVV"
BASE COUNT         1156 a         2497 c         2486 g         1088 t
ORIGIN      
        1 gccgcccccg cgcctgcccc ccgggcacgc gagccgggca agcccgtgaa acccaggccc
       61 cggccagcgc cggcggcccc acccgccgcc accgaggaca gcccggccga cagcccggtg
      121 cagtccgccc cgcctgcgcc gatcatctcc cccatggagg ccgaggccgc ccctgcgtcc
      181 accgaggcgg caatgccagg cagcgatagc caggccgcgc cgcaggtgcc gccaaccacc
      241 gccgaagcgc ccgaacccga caaggccgcc gcagccgaac ccgaaaaggc tgccgcggca
      301 gagtcggccg agaccgccgg tgtcgagatc cgccccccgg gccagccggg cgcgcgcgcc
      361 agcagcgagc cgccgccggt gctgctgccg ccctccacgc gcctgtcgtt cgacgtggtg
      421 ggcgaggtga aaaaattcca ctacaacgcc agcgccgagc tgctgtggcg ccagcagggc
      481 cagcgctacg aggcgcgcca gcagatcaag gccttcctgc tcggcgcgcg cacgcagacc
      541 agcgtgggca gcatcacgcc ccagggcctg cagcccgagc gctttggcga caagtcacgc
      601 agcgagcggg cggcgcattt cgacttcgag cgccacgagg tcatcttcag cgccaacacc
      661 ccgcgcgcca ccatcggcgc gggggcgcag gacaggctca gcgtgttcct gcagctgggt
      721 gccatgctgg ccgccgcgcc agagcgctac ccgccaggca cgcagatcac gctgaccacc
      781 gtgggcgcgc gccacgccga ccgctggact ttcagcgtcc aggggccgga gacgctggac
      841 ctgcccgccg gcagcacgcc ggccctgaag ctcgaacgcc tgccgcaggc ggatcagcag
      901 cgcgaccaga aggccgagct gtggctgggc acggcgctgc actatctgcc ggtgcgcata
      961 cgcctgacgc agggcaatgg cgactttgcc gacctgctgc tcagcggcca cgaagcgcca
     1021 tgaccacagc cggcctgggg ccatggcgcg aatccgggcg cggtacggca cgaggggggt
     1081 ttccatggaa tactggctgc atatccggtg gcccttgaac tcttgccagg cgttgccatc
     1141 tgatagccat acttataggg ggatcgcgcc atgaaaatgc tctacgactc tgactctttt
     1201 gtggtggttc acatgctgcc agacgccggc ctgagcgaca gcgccgcgaa aaccgcccca
     1261 ttgccgcccc agctgctgcg ccacggcttc gaaatcgtgg acaagcgctc gggcaaggag
     1321 gtgtacctgg acggctcctg ggccgagctg ttccagcagc agatcctggc ctggcagcgt
     1381 gatacgccga cccaggagga ggtggaagac acgctggaca gctacgtcgg actggcgcag
     1441 aatcccgtcg tagtacattg agtttcgggg gtctttaccc cctttgcggc ccacccccgg
     1501 taggccgcag ccccgcgagg ggcacagcgc gccgtggccg accgccacgg cgccttcgtt
     1561 ttgggcgcca accaatttgc cgggctcgcc aggttgttta gtcaaatacg cccgtagcgc
     1621 ccatgcataa agcgccaaaa gctattgttt aaatagcaaa acaactttag cgcaccccac
     1681 ggcgccgcgc catctcctgg cccatggcgc tctgggcacc cggatcgagc agcgcgcgcg
     1741 ccgcggggta gatgctgtcg tcctcatggc gcagatggtc ggcgtagcac tcggcaaatc
     1801 cgtccagcgc cgcctcatgc tcggcgctga acgccggtaa ctgcccatcg gccatggcct
     1861 gcagcgggcc gcgcgcggtg gcccagcgct cggccatgcg cacatggtcg cgctgcagct
     1921 ggcgcaccag ggtcaccagg ggcgcgtcat gctcctgggc cagcagcggc ggaaagacat
     1981 gcagctcctc gtcctcatgg tgcagcggcg cggcgatgtc gaagtagcgc agcacgtcgc
     2041 gcgccgcctg gcgcgccgag tcgtcgcagc cgtggccgtg gccgcgcagg tagtcgcgca
     2101 ggcgccgcag cagatccagc gagcggcgca cgcggtcatg gcaggcttcc agcatctcaa
     2161 agggctggtc gaagcctgcg cccgggctgc gcatgccggg cagggatact ctggtcgcca
     2221 tgggcaacta tcctagccgc acgggttggc cctcccggtt gaatggcagg taggcgtcaa
     2281 aggcgccggt cttcacaccc tccaccatgc ggccgagcac gcgctcggca tcgtcctcgg
     2341 acggcgcccg ggtgggggtg cccgacagcg ccgcggtgaa taccaggcgc cagggctggc
     2401 ccggcggctc gaactgccgc gactcgcgca gcagcgcggc aaacgattcc agctcctcgg
     2461 gcagcctgtc caggcacatc atgggcacca ggacaccgcc atcgccctgc gcaaagcgct
     2521 cgcgttggcc gggcgtggcg tcctcgggca gctcggtgcc ggcaaacacc agcagcaggc
     2581 gctggcgctc gcgctgcgcg cgcgcggcgt gcagcaggtc gtcaaagtgg ttgatttcgg
     2641 tcttcatggc catgccctcc cttgtccatc cacggctgca ggcccacgcc cggcaccagc
     2701 gtggacacgt actgcgccgg caccgggcgc acgcgcgcgg gcttgcgcgc gagcaccgtg
     2761 ccgacgctga caaaacacag ggcacgctcg ccagtcccga gcgcaaacag cgcgcgcagc
     2821 ccctgtgact gcagcgcctt gccactggtc agcgccgagc caaagcccag ggccgtggcc
     2881 atgagcagca ggttctgcac cgcgcagccg gccgatacca ggcgctcggc cggcacgatg
     2941 tcggggtcgc cccccagctc atcgaccgcc agcaacagca gcagcggggc gcgaaaggcc
     3001 ttttcgcgcg cctgctcctg ctgctcgggc gtggccgcgc tgtcgcgctc gcacaaagcc
     3061 tgggcaaaca cctcggccag cgccgcgcgc gcctcttgcg gcacgagcac gaagcgccag
     3121 ggcagcagtc ggccatgatc cggcgcatgg ccggccgccg ccagcatggc ctgcagctgc
     3181 gcggcatcgg gcccgggtgc gcccaggcgc ttgggcaata tggtctggcg cgactgcagc
     3241 agggccgcgg ccagatcggc ggccgaagcg ccgcccatcg ccggccatgg cgtggcggca
     3301 tcctgttcat cactgcacaa gcgtttgtcc atcatgttcc acatcgggcg ggccacagcc
     3361 cggtacccca ttttggctgc gctattgcac caaggggctg tcggcggcat cgagctgcac
     3421 gccttcgacc tcggcgctgc ccgccagcag ctgcaggtat tggcgcagcg cattcaccca
     3481 ggcctgctgg cgcagcacca gcgcgatcga ggcctgcacc tggtcaaacg cctggggctg
     3541 ggccggcttg cgcgcgcaaa cctgcaccac atgcaggcca aatcgactgt gcaccaggcg
     3601 cgcgagcacg ccgacctcct gcgagccaaa gacctcgcgc gcaaactcgg gcgcgcagtc
     3661 ctcgggcgtg agccagccca ggtcgccgcc ctgggcgccg ctggggcagt tggaccattc
     3721 gcccgccgcc tcggcaaagc gcgggccgcc gtcgtcggcg cagcgcagct ccagcagcag
     3781 gcgctcggcg cgctggcgca gcaggctcac gtcgacgccg ggcgtgacgg cgaacagcac
     3841 atggcgcagg tgggcgcgct cgccctggct gccaaaaccc ggatgggcgg cgtggtagcg
     3901 ccggcaggcg gcctcggagg gctcgggcac ctgcagggcc tgctccagca gccgctctat
     3961 cgcctgggcc gcggcggcgc tggtggcgcc gtccaggccg ggcgcgtcgt cctcggccag
     4021 caggccctgg cgctgcgcct gctggcgcag caactcggtg caggcgcgct ggcgcagcat
     4081 atcgccgggc ggctgcgcgc ccgcggcgtg cagggcgacg ccgttgatgc gcgccacttc
     4141 tgcctggtcg ctggcactgt cactggcact ggcactgccg cagccacagg catgggttcc
     4201 acaggtactg gtcatgggtc tctcctggca ggccccttgc gccatgccgg cggcatttgg
     4261 agccaaattg gcctgcagcg cttatctaca aagcgcaaac agctattatt tttacagtga
     4321 acgtcgtggc tcgttctggc ccggcggcac gttcaggcgc cgcgcgcgca ccagctgata
     4381 ggggcgcagc acataggcca gcgtgccaaa gccactccag acatgcacca ggcgcgtgaa
     4441 cgggaagatc aggaacaccg tcatgcccag gaacaggtgg gccttgaaga cccagcccgc
     4501 gccggccagc atctccaccg cgccggaacg gaaggtgacg atgcgctgac cccattcggc
     4561 caggcgcatc atcatcgagc cgtccaggtg ctgggccgac agcgggatgg tggccaggcc
     4621 cagcgccagc tgaacccaga agatccacag cagcgcgatg tcgctggtct tggaggtcgc
     4681 ccggatgcga gcgtccgtta ggcgccggtg cagcagcagg gtcacgccga cgaaggccat
     4741 caggccggca atgccaccgc tgaccatggc cagcagttgc ttgtcgtgcg cggtgatgac
     4801 gtagtcgtag aggacatgcg gcgtgagcat gccgacgaag tggccgaaga acaggaacag
     4861 cacgccgacg tggaacaggt tgctggccca gcgcaggctg ccggtgcgca gcagctgcga
     4921 ggagtcgctc ttccacgtgt actggtcgcg gtcaaagcgc agccagctgc cgatgaagaa
     4981 cacggccagg caaatgtagg gatacaggcc gaagaggaaa tggtcgatcc aggcggtcat
     5041 ggcgagactc cttgggaaga ttgcgggcgc gccttgcgca cgatgtgaat cggttggggc
     5101 tggtcggggc ggttctggcc gcgcgtggcg cagccgtcga aggcctcggg ctcggcccag
     5161 gcctcgtcca tgtcgggctc gggggcgatg gcgaccgcct gcgcggtctc gccggcagcc
     5221 tcgagcacgg cggcgagcac gctggcgtag gggctggcct tggcctgcag cgcgctgaac
     5281 agcgcattca ggatatgcgc catctcggcg agaaactcgc gcgccaccgc ggtcggctgg
     5341 gtcgaggcga actccagcac cacgcccagg tggtcgggca gctcctgcgc gtccaggtac
     5401 aggccggcgc gctcgtaggt ctgcgtcagg tcgatcagcg ccgggccgcg ttcgcgcgag
     5461 tcgccatgga tatgctcgaa caggtgcagc gaggtgcggt ggccgcggtc gaaggtatcg
     5521 acatagcgcc cctcggcctc gtaggcgtcc atggcgcaga tctgctgggc cagggcctgc
     5581 agctcggcga tgcgggccgc gggcagggcc tgctcggtct gcagcgcgtc gatcaatgcg
     5641 ggcatctgcg cgcgcagccc ggcgccgggg taggccagca gccgcgccag cgcgcgcagc
     5701 gtcaggcgca tggtctcggg tgtcttcttg aacatggtgg cgctcctcag accacggcct
     5761 tgatgggaat cgtgcgcggc ttcttgccgc cgaagatgct cacctcggtg gctccgtcgg
     5821 agcagccgtt gccgaaggag aagccgcagc cgccgcgcac gtcaaaggcg tcctcggcgt
     5881 actcgcggtg ggtggacggg atcacgaagc gatcctcgta gttggcaatc gccatcacct
     5941 ggtacatgtc ctccacgtcg tgcaccgtca ggcccgtctg ctgcagcacg gccatgttct
     6001 gcaccttgtc cacatgcacg ccgcgctggt aggcgcgcat ggccagcatg cgctcgagcg
     6061 cgcgcaccac cgggccggtg tcgccggcgg tcagcaggtt ggccaggtac tgcacgggga
     6121 tgcgcagcgc cgagacgtcg gggatctcgc cattcacgcc cacatggccg gcgttggccg
     6181 ccgaggtgat gggcgagagc ggcggcacat accagaccat gggcagggtg cggtactcgg
     6241 ggtgcagcgg cagggccacc ttccagtcca cggccatctt gtagactggg ctgttcttcg
     6301 ccgcctccag ccagttgtcg ggaatgccgt cgagccgcgc ctggcgtatc acctcggggt
     6361 catgcgggtc caggaagatg tcgagctgcg cctggtacaa gtccttgtcg cgctccacgc
     6421 tggccgcttc ctggatgcgg tcggcgtcgt agagcagcac gccaagatag cggatgcggc
     6481 cgacgcaggt ctcggagcag accgtgggct ggccggcctc gatgcgcgga tagcagaaga
     6541 tgcatttctc ggccttgccg gtctgccagt tgtagtagat cttcttgtac gggcaacccg
     6601 agacgcacat gcgccagccg cggcatttgt cctggtcgat gagcacgatg ccgtcttcct
     6661 cgcgcttgta gatcgagccg ctggggcagc tggccacgca ggccgggttc aggcagtgct
     6721 cgcacaggcg cggcaggtac atcatgaagg tgttctcgaa ctgggcgtac atgtcctttt
     6781 gcacctgttc gaagttcacg tcggcgctgc gcctggagaa ctcgccgccc aggatttctt
     6841 cccagttcgg accccagacg atcttctcca gccgctcgcc ggtaatcagg ctgcgcgggc
     6901 gcgccaccgg ggtgttcttc atctcgggcg ccgactgcag gtggtcgtag tcgaaggtga
     6961 agggttcgta gtagtcgtcg atctgcggca ggttcgggtt ggcgaagatg cgcatcaaga
     7021 gcttccactt gccgccctgg cgcggcgtga tgctgccgtc ggcatggcgc gtccagccgc
     7081 cgttccactt gtcctggttc tcccattcct tggggtagcc gatgccgggc ttggtctcga
     7141 cgttgttgaa ccaggcgtat tccatgccgg gccggctggt ccagacgttc ttgcaggtga
     7201 cggaacaggt gtggcagcca atgcact
//
LOCUS       DAOMTQ010000147         7183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2390_length_7183_cov_25.466470, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000147 DAOMTQ010000000
VERSION     DAOMTQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_7183_cov_25.466470"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK518_12535"
     CDS             complement(<1..405)
                     /locus_tag="PK518_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM49784.1"
                     /translation="MSNTVPLPPATEMQQAILQHHGAAFADFPAQPAPAPESDEPVYR
                     AALAACSALGFIAQDAGCLARAWAAQTRRLGFFDAAHWPDEPADFGLQPRPHAQPFAP
                     CPQALGLYAVLPDAAWVGRMARAGVPTVQLRYK"
     gene            complement(408..1211)
                     /locus_tag="PK518_12540"
     CDS             complement(408..1211)
                     /locus_tag="PK518_12540"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRM49785.1"
                     /translation="MTTDPLVLYGQSFASRLLLGTARYPSPAVLQAAVQRARPAMVTA
                     SLRRQGINATNAGNSFWELLKSLAVPVLPNTAGCHSVQEAVTTAQMAREVFDTPWIKL
                     EVIGDDYTLQPDTQQTVEAAAILIKDGFQVLPYCTEDLVQCQRLIDVGCQAVMPWAAP
                     IGTGRGPMNPYALQLLRERLNVPLLVDAGLGLPSHAAQVMEWGFDGVLLNTAVALSQD
                     PVAMAGAFADAVAAGRAARLAGAMEQHSSAQPSTPVLGTPFWHHDKAQA"
     gene            complement(1270..1467)
                     /gene="thiS"
                     /locus_tag="PK518_12545"
     CDS             complement(1270..1467)
                     /gene="thiS"
                     /locus_tag="PK518_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRM49786.1"
                     /translation="MKILLNQQSTDLPAQASVQDALALLAPRPPFAVAVNTVFVPKAQ
                     YPSHLLQDGDRVEVIAPVTGG"
     gene            complement(1464..2570)
                     /locus_tag="PK518_12550"
     CDS             complement(1464..2570)
                     /locus_tag="PK518_12550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRM49787.1"
                     /translation="MQPLLSPSSSVTILGAGLLGSLLATSLARQGHRVQVFEAGGPDG
                     VGSAAYVAAAMVAPLAESVVAEASIVRMGQHALARWPELIAPLPAPVYWQQNGTLIVW
                     HRQDAGDAARLRGLFEHRQAEVAGLPAMQPLDAAGVAAADPALAGRFAQGLLLPGEGQ
                     IDNRQLFAALAQDMQQLGVAVHYDSPRELGDFRPGEAGQSDWLIDCRGLGARQQWQAL
                     RGVRGEVVRIHAPEVTLARPTRLVHPRYSIYVAPKENHVFVIGATEIESDDRSGMSVR
                     SALELLSAAYTIHSGFAEARILELSTQCRPTLPDNLPGIRMPQPRVLQINGMYRHGFM
                     QAPALHDVALQWLNQGQSPLAAQFDLALQGTAVT"
     regulatory      complement(2635..2738)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2817..3656
                     /gene="thiD"
                     /locus_tag="PK518_12555"
     CDS             2817..3656
                     /gene="thiD"
                     /locus_tag="PK518_12555"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRM49788.1"
                     /translation="MNHSNTPAPRYARVLSIAGSDSGGGAGIQADLKTFSALGCYGMT
                     AITAITAQNTQGVRAIHGVPPDILRAQIDAVVEDIGVDAVKIGMLHAPEVVQVVADAI
                     RRHQLPNVVLDPVMVATSGDRLIAEETVGQLVRELFPLAALVTPNLDEGGWLLGRQIT
                     REDELDAAADELLALGARAVLLKGGHLRGERVVDLLALPGGEHHRLASARIATHNGHG
                     TGCTLSSAIAAQLALGLDLVAAVQAARGYILGAIAAGAAVHTGRGHGPLNHGYAPVEQ
                     VIL"
     gene            3763..4725
                     /locus_tag="PK518_12560"
     CDS             3763..4725
                     /locus_tag="PK518_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRM49789.1"
                     /translation="MSQNVIQFQHGLSWAQFHDCYGSEAQCEAAVVAARWPRGWRCAH
                     GGCKRFFHTRNGHGRLLWECFVCGYQSSSIAGTVMEHTKVPLTKWFQAMYLLTQNKNT
                     LSALALSRQIGVSYRTAWLLKHKLMQTMLLREESRPLQGRVEIDDAYLGGEREGHING
                     GRKAANKTAFVAAIQTSDDGHPLCMRLTPVVDFTNADMAPWAQGHLAPVCHVVSDVTW
                     AFAQVVQAAATHERHVTGGGRQGARTPQLRWVNTLLGNLKTSLTGTLHSFDHARYAAR
                     YLAEFAYRFNRRFDMPAMIPRLLRAVTVTKPQPLRSLRVCGVCT"
     gene            complement(4788..4967)
                     /locus_tag="PK518_12565"
                     /pseudo
     CDS             complement(4788..4967)
                     /locus_tag="PK518_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557982.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(4995..6080)
                     /locus_tag="PK518_12570"
     CDS             complement(4995..6080)
                     /locus_tag="PK518_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49790.1"
                     /translation="MPESINGVDLGVSVVLHPPLCQFSPLERHPKMTSATRRDILKAA
                     AAGTFLSTLASPYAMALASAWPTRAVNLVVGYPPGGLTDAGARFISNGMTKSLGQPVI
                     VDNKPGASGNVAANEVLRTPGGLKLLVANPSLTINPHTFSAPSPDPTAFTPIGLILES
                     QYILCVHPSAPTKNLAEFVAWAKAESNKGFSYATAGAGGNTHLMMEYFRERMGLPKLS
                     PVPYKGSAPAIQDVVGGQVPCLLDAASLLIPFIATGKLRPILVTGSTRHPALPDVPTA
                     TELGIKDFNMTAFIGLWGPPNLPADIVQKVNTAMNFALADPAIRDTITKNGDSVGGGT
                     PERLDTITKANYKLWGEVAKRNNIRSE"
     gene            complement(6118..6309)
                     /locus_tag="PK518_12575"
                     /pseudo
     CDS             complement(6118..6309)
                     /locus_tag="PK518_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6419..>7183)
                     /locus_tag="PK518_12580"
     CDS             complement(6419..>7183)
                     /locus_tag="PK518_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRM49791.1"
                     /translation="LVSEGVDVNRVLCNMRVDGTLQSAVAQADLVIEAAPEKLELKQK
                     LFADVAAAAPRHAVLASNTSVIPITRIGEALEPQARARLVGTHWWNPPHLVPLVEVVR
                     TAYTSEEVFEQTFAWLAAAGKCPVRVQHDVTGFIGNRLQHALWREAIALVEDGVCDAE
                     AIDLVVKNSFGMRMPVLGPMENADLVGLELTRDVHRVMFPDLKCDKTPSALLGRLIEK
                     GSTGMKAGQGLRAWTPEQAQEVHARLAAHLIRMTRD"
BASE COUNT         1227 a         2227 c         2428 g         1301 t
ORIGIN      
        1 cttgtagcgc agctgcaccg tgggcacgcc ggcgcgcgcc atgcggccga cccaggcggc
       61 atcgggcagc accgcgtaca ggccaagggc ctgggggcag ggggcaaagg gctgggcgtg
      121 cgggcgtggc tgcaggccaa agtccgcagg ttcgtcaggc caatgtgcgg catcgaaaaa
      181 ccccaggcgg cgcgtctgtg cggcccaggc gcgcgccagg cagccggcat cctgggcgat
      241 gaagcccagg gcgctgcagg cggccagcgc ggcgcggtag accggctcgt cggactcggg
      301 tgcgggcgcg ggttgtgcgg gaaagtcggc aaaggccgcg ccgtggtgct gcaggatggc
      361 ctgctgcatc tcggtggcgg gcggcagggg gacggtgttg ctcatgttca ggcctgcgcc
      421 ttgtcgtggt gccagaacgg cgtgcccagc accggtgtgc tgggctgggc actgctgtgc
      481 tgctccatgg caccggccag gcgggcggcg cggccggcgg ccacggcgtc ggcaaaggcg
      541 ccggccatgg ccacggggtc ttgcgacagg gccacggccg tgttcagcag cacgccgtca
      601 aaaccccatt ccatgacctg ggcggcgtgc gagggcaggc ccaggccggc gtccaccagc
      661 agcggcacgt tcaggcgctc gcgcagcagc tgcagggcgt aggggttcat ggggccgcgg
      721 cccgtgccga tcggggcggc ccagggcatg acggcctggc agcccacgtc gatcaggcgc
      781 tggcattgca ccagatcttc ggtgcagtag ggcagtacct gaaaaccgtc cttgatgagg
      841 atggcggcag cctccacggt ttgctgggtg tcgggctgca gggtgtagtc gtcgccgatc
      901 acctccagct tgatccaggg cgtgtcgaag acctcgcgcg ccatctgcgc cgtggtcacg
      961 gcctcctgca cgctgtggca gccagccgtg ttgggcaaca ccggcacggc caggcttttg
     1021 agcagctccc agaagctgtt gccggcgttg gtggcgttga tgccctggcg gcgcagcgat
     1081 gccgtgacca tggccggccg cgcgcgctgc acggccgctt gcagcacggc cggcgaaggg
     1141 tagcgcgccg tgccaagcag caggcggctg gcaaagctct ggccgtagag gacaaggggg
     1201 tctgtagtca tgttttcaat cctgtattga tagctgctgg cgcttgtttg atgggcgtta
     1261 gaggcagttt taaccaccag tgacgggagc aatcacctct acccggtcac cgtcctgcaa
     1321 caggtggctg gggtattgcg ccttgggcac gaacaccgtg ttcacggcca cggcaaaggg
     1381 cgggcgcggt gccagcaggg cgagcgcgtc ctgtacgctg gcctgcgccg gcagatcggt
     1441 ggattgttgg ttcagcaaaa tcttcatgta acagcggtcc cctgcagggc caggtcgaat
     1501 tgggcggcca gcggcgattg tccctggttc agccattgca gcgccacgtc gtgcagcgcg
     1561 ggcgcctgca tgaagccgtg gcggtacatg ccgttgatct gcaggacgcg cggctgcggc
     1621 atgcggatcc cgggcaggtt gtcgggcagg gtggggcggc actgcgtgct cagctccagg
     1681 atgcgcgcct cggcaaagcc gctgtggatg gtgtaggcgg ccgagagcag ctccagggcc
     1741 gagcgcacgc tcatgccgga gcggtcgtcg gactcgatct cggtggcgcc gatgacgaac
     1801 acatggtttt ccttgggcgc cacatagatc gagtagcgtg ggtggaccag gcgcgtgggc
     1861 cgcgccagcg tcacctcggg cgcgtggatg cgcaccacct cgccgcgtac gccgcgcagc
     1921 gcctgccatt gctggcgtgc gcccaggcca cggcagtcga taagccagtc gctctggccc
     1981 gcctcgccgg ggcggaagtc gcccagctcg cgtgggctgt cgtaatgcac cgccacaccc
     2041 agctgctgca tgtcctgggc cagcgcggca aacagctggc ggttgtcgat ctgcccctcg
     2101 cccggcagca gcaggccctg ggcaaagcgg ccggccaggg ccgggtcggc ggcggcaaca
     2161 cctgcggcat caaggggctg catggcgggc aggccggcaa cctcggcctg gcgatgctcg
     2221 aacaggccgc gcaggcgcgc cgcgtcgccc gcgtcctggc ggtgccagac gatgagcgtg
     2281 ccgttttgct gccagtacac cggcgcgggc agcggcgcga tcagctccgg ccagcgcgcg
     2341 agcgcatgct ggcccatgcg cacgatgctg gcctcggcca ccaccgattc ggccagaggc
     2401 gccaccatgg ccgcggccac ataggcggcc gatcccacgc cgtcggggcc gccggcctcg
     2461 aaaacctgca cgcggtggcc ctggcgcgcc aggctggtgg ccagcaggct gcccagcagg
     2521 cccgcgccca ggatggtgac cgacgaagag ggagaaagca gtggctgcat ggtgttttgt
     2581 ccgctatcca atcaaaaccg tggggggaca aaacaggcgc caagggcggc gtggccctgg
     2641 tggttgaaaa ctccccacgc cagcatgacc tggatcgggt gcggagggtg tttctcagtc
     2701 gccagcctgg ttgggccggg acacccctgt ttcgtccgtg agccgggatt acagcacaga
     2761 aggcaagccc atgggattga cctgggacag agcttgcatc cgccataatt tttgccatga
     2821 accactccaa caccccggcg ccgcgttatg cgcgcgtgct gtccatcgct ggctccgaca
     2881 gcggtggcgg cgcaggcatt caggccgacc tcaagacctt cagtgcgctt ggctgctatg
     2941 gcatgacggc catcaccgcc atcacggcgc agaacaccca gggggtgcgc gccatccacg
     3001 gcgtgccccc ggacatcctg cgtgcgcaga tcgatgccgt ggtcgaggac ataggcgtcg
     3061 atgccgtgaa gatcggcatg ctgcacgcgc ccgaggtggt gcaggtggtg gccgatgcca
     3121 tacgccgcca ccagctgccc aatgtggtgc tggacccggt gatggtggcc accagcggcg
     3181 accgactgat tgccgaggaa accgtgggcc agctggtgcg cgagctgttt ccgctggccg
     3241 cgctggtcac acccaacctg gacgagggcg gctggctgct ggggcgccag atcacgcgcg
     3301 aggacgagct ggatgcggcg gcggatgagc tgctggccct gggcgcgcgc gccgtgctgc
     3361 tcaagggcgg tcacctgcgg ggcgagcgcg tggtggacct gctggccctg cccgggggcg
     3421 agcaccatcg cctggcttcg gcgcgcattg cgacgcataa cggccacggc acgggctgca
     3481 cgctgtcgtc ggcgatcgcg gcgcagctgg cgctgggcct ggatctggtg gccgccgtgc
     3541 aggcggcgcg cggctacatc ctgggcgcca tcgccgcagg cgccgcggtg cacacgggcc
     3601 ggggccatgg gccgctgaac catggctatg cgccggtgga gcaggtcatt ttgtaggtat
     3661 ttttacctga ttaggcacat atttcagacg aaccactgct cgctctgggc atcaaaccag
     3721 caagagcgtt ttgctcgatt gcgaggcagc aggaggtgtg tgatgtccca gaacgtcatc
     3781 caattccagc atggtctgtc gtgggcgcag tttcatgact gctacggcag tgaggcccaa
     3841 tgcgaagctg ctgtggtggc agcacgctgg ccgcggggct ggcgctgcgc gcacggcggc
     3901 tgcaaacggt ttttccatac acgcaacggc catgggcgcc tgctgtggga atgctttgtc
     3961 tgcggctacc aaagctccag cattgcgggc accgtcatgg agcacaccaa agtgccactg
     4021 accaagtggt tccaggccat gtacctgctg acgcagaaca aaaacaccct cagtgccctg
     4081 gcgctgagcc gccagatcgg cgtgtcctac cgaactgcct ggctgctcaa gcacaagctg
     4141 atgcagacca tgctgctgcg cgaagaatcg cgccctttgc aaggccgtgt ggaaatcgac
     4201 gacgcttact tgggcggcga acgcgagggg cacatcaacg gcgggcgcaa ggcggccaac
     4261 aaaacagcct ttgttgctgc catacaaacc agcgatgacg ggcatccttt gtgcatgcgc
     4321 ctgacaccgg tggtggattt caccaacgca gacatggccc cctgggccca aggccatctg
     4381 gcccctgttt gccatgtggt cagcgatgtc acatgggcct ttgcacaagt ggtgcaggcg
     4441 gcagctacgc atgaacgcca cgtcaccggc gggggcagac aaggcgccag aacaccgcag
     4501 ctgcgctggg tcaacacctt gctgggcaat ctcaagacca gtctgacggg tacgctgcac
     4561 tcgtttgacc atgccaggta cgccgctcgc tacctggccg agttcgcgta ccgcttcaat
     4621 cggcgctttg acatgcccgc catgattccc aggctcctgc gggccgtcac ggttacaaaa
     4681 ccccagcctc tgcgctcgtt gcgtgtgtgt ggggtatgta cctaatcagg tatttttaag
     4741 gctgcagcga ttgtctggca agcgcaagca gctataaaaa tagtggctca agtagcgata
     4801 tctattgatt ggctcccaat gtccataacc cgcagggcag caggcttggt cacgatgtgg
     4861 agagcacggt ggattgttct gaaccagccc agggaggaat ccaagtattt ggccgccacc
     4921 cctttgaacg gacgcatcca ggccttcaag cggctgtggt gggctgaagt tacagttctg
     4981 gccgcctacg cggcctactc cgaacgaatg ttgtttcgct tcgcgacttc tccccaaagc
     5041 ttgtagttcg ctttggtgat tgtgtcgagc cgttcaggtg tgcctcctcc cacagaatcc
     5101 ccgtttttgg tgatcgtgtc tctgatcgcc ggatcggcca acgcaaagtt catcgcggta
     5161 ttgaccttct gcacgatgtc agcgggaagg ttcggtgggc cccacaggcc gataaaggcc
     5221 gtcatgttga aatccttgat gcccaattcg gtcgccgtcg gcacatcagg cagtgcagga
     5281 tggcgtgtgc tgcctgtcac aaggatggga cgcagcttcc ctgttgcaat aaacgggatc
     5341 aggagcgacg ctgcatcaag caagcatggc acttgccccc ccacaacgtc ttgaatcgca
     5401 ggggcactgc ccttgtacgg caccgggctg agtttgggca aacccatccg ctctcggaag
     5461 tactccatca tcagatgggt gttgccgccg gcgccggccg tcgcgtagct gaatcccttg
     5521 ttgctctctg cttttgccca agcaacgaac tccgccaagt tcttggtagg cgcggatgga
     5581 tggacgcaaa gtatgtactg cgactccagt atcagtccga tcggtgtgaa cgcagtggga
     5641 tctggcgacg gggcagaaaa ggtgtgggga ttgatggtta gacttggatt ggcaaccaac
     5701 agcttgagcc cacctggcgt gcgcaggact tcattggcag ccacgttacc ggatgctccg
     5761 ggcttgttgt caacgatcac cggctgtccc agcgatttcg tcattccgtt gctgatgaag
     5821 cgcgcgccgg catcggtcag gccgcccggg gggtacccca cgaccaggtt gaccgctcgc
     5881 gttggccagg ctgaggccag tgccatggcg tacggcgatg cgagtgtcga caggaaagtg
     5941 cctgccgcag cagctttcaa gatgtcacgt cgggttgctg aagtcatttt gggatgtctc
     6001 tctaaaggtg aaaattggca gagcggtgga tgcaggacga cgctgacccc gaggtcaacg
     6061 ccattgattg attcgggcat gcatgggatc gatagttgtc tcctgtttcg cgcctcaggc
     6121 cgcgctgtag aacgtgccga acgtttgctc gtggtgttcc gtctgatcca cgacgacgca
     6181 gtcgaccagg acgccggaga tcttgacctt gcgcgggttt agcgctcctt gcgtcgcaat
     6241 gcgccctgct tgcacgatca cgagatcgcc actgttgcgc gccgccatgg ccagcgggag
     6301 catttgcagt gccggcgccg atggcgctgg tcgagccggt gaccagggta gatttacctt
     6361 gcagcatggg tgtctccacg tttattcgtt cgttctggcg caactaagca ccgagagatc
     6421 aatcgcgggt catgcggatg aggtgcgcag ccagtcgagc gtgcacctct tgggcctgct
     6481 cgggcgtcca ggcgcgcagc ccttggccgg ccttcatgcc ggtgcttcct ttctcgatca
     6541 agcgaccgag caatgccgag ggggttttgt cgcacttaag atccggaaac atcacgcggt
     6601 gcacatcgcg cgtcagttct agaccgacca ggtcggcgtt ttccatgggc cctaataccg
     6661 gcattcgcat gccaaagctg ttcttgacca ccagatcgat ggcctcggca tcgcacacgc
     6721 cgtcttcgac caaagcgatg gcctcgcgcc acagtgcgtg ctgcaaccgg ttgccgatga
     6781 aacccgtcac gtcgtgctgc acgcgtaccg ggcacttgcc agcggcagcc agccaggcga
     6841 acgtctgttc gaacacttct tcgctggtgt aagcggtgcg cactacttca accagtggca
     6901 ccaagtgcgg cgggttccac cagtgcgtac cgaccagccg cgcgcgggcc tggggctcca
     6961 gcgcctcgcc gatgcgcgtg attggaatga ccgaggtgtt gctggccagc accgcatgcc
     7021 tgggtgcagc agcggcgacg tctgcgaaca gcttctgctt gagttcgagc ttctcgggcg
     7081 cagcttcgat caccaggtcg gcctgagcga ccgcgctctg cagcgtgccg tcgacgcgca
     7141 tgttgcacaa cacgcgattc acatcgaccc cctcggagac tag
//
LOCUS       DAOMTQ010000148         7159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2401_length_7159_cov_28.370214, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000148 DAOMTQ010000000
VERSION     DAOMTQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_7159_cov_28.370214"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1376
                     /gene="nrdD"
                     /locus_tag="PK518_12585"
                     /pseudo
     CDS             247..1376
                     /gene="nrdD"
                     /locus_tag="PK518_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
     assembly_gap    1397..1496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1688..1870
                     /gene="nrdD"
                     /locus_tag="PK518_12590"
     CDS             1688..1870
                     /gene="nrdD"
                     /locus_tag="PK518_12590"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM49792.1"
                     /translation="MTHFSAIESTALAAADVQLSNAERQPCEVWTRVMGYHRPVASFN
                     IGKQGEHAERQFFAEQ"
     gene            1867..2541
                     /locus_tag="PK518_12595"
     CDS             1867..2541
                     /locus_tag="PK518_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRM49793.1"
                     /translation="MTLRVGGLTRLTTIDFPGRLAAVVFCQGCPWRCGYCHNPELLDA
                     SAGGAIAWESVLAFLHGRRGLLDGVVFSGGEPLAQSALPQALAQVRAMGFATALHTGG
                     MYPDRLAAVLPLLDWVGLDIKGPQAGYDAITRTPGSGARAWQSLQLLLASGVDYECRT
                     TWHEGLFDTPGLLGLAEQLARAGVVHWALQECRTPDGPPTWGLTPAQQTLLQAQFPGF
                     VLRSGL"
     gene            2622..2990
                     /locus_tag="PK518_12600"
     CDS             2622..2990
                     /locus_tag="PK518_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49794.1"
                     /translation="MSTALKSPESARIDIFTQAVESLARKLGVAASETPMPISKFKSD
                     YSGLLRDARAGQLQQISRGGERYLVLSEAQVIAMERSSSQQRSLADTLASVQAPSGAL
                     DAGAALLPGERHDPFTLPRA"
     gene            3094..3552
                     /locus_tag="PK518_12605"
     CDS             3094..3552
                     /locus_tag="PK518_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49795.1"
                     /translation="MARPNRYLLGPQLLLGLCGKQARAVQWAAGVQEAECVLSRLAIA
                     IARSVINSGARSEVQRQDWHQALAATVSRMEAGGATLVDLNETVLERFHEYRTHAPLD
                     FKTPKGRAPLAQDVRLLITTAAVMDLVYAEYDDLYMEQLRSGTNVRVQAL"
     gene            3549..4553
                     /locus_tag="PK518_12610"
     CDS             3549..4553
                     /locus_tag="PK518_12610"
                     /inference="COORDINATES: protein motif:HMM:NF041079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS cGAMP-activated phospholipase"
                     /protein_id="HRM49796.1"
                     /translation="MSAPEDEPFTLPAGLPRAAPGRVRVLSLSGGGYRGLFTAALIRA
                     LEARLGPDPARPTPFGQRFDLIAGTSIGGILATALSCRATGAALQALLQRRGSSIFPP
                     MRLRGLRKLLGRAPYDPQRLRSAICELLPDAEQQLLGEHAPALLLTTVNWTTSKLQLL
                     GSRPTLGQRDQLGLTLMDAMLATSAAPAHFPVHSFQHHRFVDGGLAANAPDLHALQCA
                     QQMYPGAEVHMLSIGTANPLHGRDPSAVPGRGLMWAGPVIELVTSAQEMLAVQECAAR
                     MGTHRYLRLNMPPSSAQQKQLDFDVADQTSTDLLLSLADDCLASLQPADWHRLKAIVG
                     "
     gene            complement(4571..4909)
                     /locus_tag="PK518_12615"
     CDS             complement(4571..4909)
                     /locus_tag="PK518_12615"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRM49797.1"
                     /translation="MFVTWAAADREQGQYKSAPSLVVEVLSPSTSAYDRGAKFAANRK
                     LPSLSEYLLIDSERLSADLFRRDETGRWVLYPFDTAAQQVELASVGLQLPLQVLYEDV
                     RLEKSASAHP"
     gene            complement(4938..5159)
                     /locus_tag="PK518_12620"
     CDS             complement(4938..5159)
                     /locus_tag="PK518_12620"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRM49798.1"
                     /translation="MALPAAQPRCDAQTFLDWEEGQAEKHQYLDGEVFAVAGASDARV
                     TIALNVAMALRQHLRGGPCSEFITDIKLR"
     gene            5381..6127
                     /locus_tag="PK518_12625"
     CDS             5381..6127
                     /locus_tag="PK518_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HRM49799.1"
                     /translation="MKSLLIATLSSLSCLAALAAPITLTGTVRDFNADGVNFEGSGGS
                     GTGYVNTTLSGSSPTLTALGSSQISNSGPGAFSNWYTKTTDSMSYSLTLNETSPGSGI
                     YQYSNSNFFPIDGALLGNQGRSHNYHFTYNISSVFSYVQGAGQTFTFTGDDDVWVFFD
                     HKLGIDLGGVHAAQSQTVNLDTLMAGKTSGDYSFDFFFAERHTTQSNLMITTSLVLKS
                     PPTNVPEPGSLALMGLSMVGLAFVRRRKTA"
     gene            complement(6320..>7159)
                     /locus_tag="PK518_12630"
     CDS             complement(6320..>7159)
                     /locus_tag="PK518_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HRM49800.1"
                     /translation="YGLQDVVNQRRMIDDGDAAEEFKAVLRGKLDALLALAEATDCRR
                     QRLLAYFGEESQPCGNCDNCLNPPAVWDGTDAARKLLSTIYRVHEVSRLTFGVGHLMD
                     IVRGKATDKVRQFGHDQLSTFNLGAQYSEQQLRGVLRQLLALGAVGVHRVATEGGHSF
                     DTLTLTPGSRAVLRGEQQVQLREAQAKAAAKPKRRAGQPSAAAADLGPDAQVRFINLK
                     AWRAEVARAHNLPAYVIFHDATLAAIAERNPASLGELEGISGMGAKKLEAYGDEVLRV
                     CGA"
BASE COUNT         1208 a         2444 c         2208 g         1199 t
ORIGIN      
        1 taagctgcct tgcgccgcgt gtctcaggca tgcggcgcat tgccaggctg caattaattt
       61 aatagctact aacgcttatc catcaagcgc tacagcctta tttactccgc aagcaaggtt
      121 ctctggcgcc ttgctttttc agccggcttc aagccggctt tttttcgttt gtggggtctg
      181 gtggaaacca gacggagagc gcaagcttgt gttgcatcaa actacatata gtgtttagag
      241 cttgcaatga cttccatttc acctttcctc gccacgctgc cgcgcgacgt tatccagaga
      301 agcggcgagc gcgctgcgtt cgacgccgga cgcattgcct cggccatcgc gcgtgccggg
      361 gccgccaccg gcgagttcgg tgctgacgag gcgcagttgc tcaccgcgca ggtcaccaag
      421 gtgctgatcc accgcttcca cggggcggcg ccggccatag agcagatcca ggatgtggtc
      481 gagcaaacac tgatcgccgc caaccacctg cagacggcgc gcgcctacat cgcctaccgc
      541 gatcggcacg ccaccctgcg tgccgaccgc cagacactgg tgcatgtgga gtcctccatc
      601 aacgaatacc tcacgcgcgc cgactggcgc gtcaacgcca acgccaacca gggctattcg
      661 ctgggcgggc tgatcctgaa cgtggcgggc aaggtgacgg ccaactactg gctctcgcat
      721 gtctacgcgc ccgagatcgg ggaggcgcac aggagcggcg acctgcacat ccacgatctg
      781 gacatgctca gcggctactg cgcgggctgg tcgctgcgca cgctgttgca cgaaggcttg
      841 aacggcgtgc cgggcaaggt ggaggcgggc ccgcccaggc acatgagctc ggccgtgggg
      901 cagatcgtca actttctggg cacgctgcag agcgagtggg ccggcgcgca ggcgttctcg
      961 tccttcgaca cctatatggc acgacctacc gctttgccaa ggaagacaaa aagcggtggc
     1021 cgggcatcgt gcaagcgggg acgcccgccc agccctacta caccaacagc tcgcagctgc
     1081 ctgtgggctt taccgacgat ccctttgagg cgctagagcg gcaagaggct ttgcaggcca
     1141 agtacaccgg cggcacggtg ctgcacctct acatgggcga gcgcatcagc agcggcgcgg
     1201 cctgccgcga actggtcaaa cgcgcgctct ccaacttccg cctgccctac atcaccgtca
     1261 cgccgacgtt ttccatctgc ccgacgcacg gctatctggc gggcgagcac cgcttctgcc
     1321 cgacttgcga cgccgagcgc ctggccgaga agcgccggca gctgcaagcg gcgtgagcgc
     1381 cgtttctccc tctcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggc
     1501 cgagaagcgc cggcagctgc aagcggcgtg agcgccgttt ctccctctcc cgctggaggg
     1561 agagggcagg ggtgagggtg acaagaaacg aggcgcagcg cttcatcacc ccctcacccc
     1621 tgccctctcc cccaaggggg gcgagggaga aataccccca tttcaaccac ccccaaagga
     1681 gctatccatg acccatttct ccgccatcga atccaccgcc ctggccgccg ccgacgtgca
     1741 gctcagcaac gccgagcgcc agccctgcga ggtctggacc cgcgtcatgg gctaccaccg
     1801 cccggtggcc agcttcaaca tcggcaagca gggcgagcac gccgagcgcc agttcttcgc
     1861 cgagcaatga ccttgcgcgt gggtgggctg acccggctca ccaccatcga cttccccggc
     1921 cgcctggcgg ccgtggtgtt ctgccagggc tgcccctggc gctgcggtta ttgccacaac
     1981 ccggagcttc tcgacgccag tgcgggcggc gccatcgcct gggagtcggt gctggccttt
     2041 ttgcacggcc gccgcggcct gctcgacggc gtggttttct ctggcggcga gccgctggcg
     2101 cagtccgccc tgccccaagc cctggcccag gtgcgcgcca tgggctttgc cacggccctg
     2161 cacaccggcg gcatgtaccc ggatcgcctg gccgccgtgc tgccactgct cgactgggtg
     2221 ggcctggaca tcaagggccc gcaggccggc tacgacgcca tcacgcgcac gccgggcagc
     2281 ggcgcgcgcg cctggcagtc gctgcagctg ctgctggcca gcggcgtgga ctacgaatgc
     2341 cgcaccacct ggcatgaggg gttgttcgac accccgggcc tgctggggct ggccgagcag
     2401 ctggcgcgtg ccggcgtggt gcattgggca ctgcaggaat gccgtacacc ggacggccca
     2461 ccgacctggg ggctgacacc cgcgcaacag accctgttac aggcgcagtt tccgggcttt
     2521 gtgctgcgga gcggcctgta gggattctct tgacatctgg tcagcttggc ctaaatatca
     2581 aggcctgacc tgaccagatt gaccagaccc ggagccgccc catgtccacc gccctgaagt
     2641 cccccgagag cgcccgcatc gatatcttca cccaggcggt ggagagcctg gcgcgcaagc
     2701 tgggcgtggc agccagcgaa acgcccatgc ccatttccaa gttcaagtcc gactacagcg
     2761 ggctgctgcg cgatgcccgg gccggccagc tgcaacagat ctcgcgcggc ggcgagcgct
     2821 acctggtgct gagcgaggcg caggtcatcg ccatggaacg ctccagcagc cagcagcgca
     2881 gcctggcgga cacgctggcc tcggtgcagg cgccgtccgg tgcgctcgat gccggcgccg
     2941 ccctgctgcc cggcgagcgg cacgatccgt tcaccctgcc ccgggcctga ttccgcttcc
     3001 aaatcattcg tgtctaggcg cgaagccgca ggcagtgctg tagcacgaca aggcgaagca
     3061 acaacgacac ggatgatcta gaaatggaat gacatggcgc gccccaaccg ttacctactc
     3121 ggcccgcagc tgctgctggg cctgtgcggc aagcaggccc gcgccgtgca atgggcggcc
     3181 ggcgtgcagg aggccgaatg cgtgctcagc cgcctggcga tcgccatcgc ccgctcggtc
     3241 atcaacagcg gggcccgctc cgaggtgcag cgccaggact ggcaccaggc gctggccgcc
     3301 accgtctcgc gcatggaggc cggcggggcc accctggtcg atctgaacga gaccgtgctc
     3361 gagcgctttc acgaataccg cacccatgcc ccgctggact tcaagacgcc caagggccgc
     3421 gcgccgctgg cccaggacgt gcgcctgttg atcaccaccg ccgccgtcat ggatctggtc
     3481 tatgccgagt atgacgacct ctacatggag cagctgcgca gcggcaccaa tgtcagggtg
     3541 caggcactgt gagcgcgccg gaagacgagc ccttcaccct gccggccggc ctgccgcgcg
     3601 ccgcgcccgg ccgcgtgcgc gtgctgagcc tgtcgggcgg cggctaccga ggcctgttca
     3661 cggcggcgct gatccgggca ctggaggcca ggctcggccc cgaccctgcc cggcccacgc
     3721 cttttgggca gcgctttgac ttgatcgccg gcacctcgat aggcggcatc ctggccacgg
     3781 ccctgagctg cagggccacg ggcgccgccc tgcaggcact gctgcagcgg cgcggcagca
     3841 gcatcttccc acccatgcgc ctgcgtggcc tgcgcaagct gctgggacgc gcgccctacg
     3901 acccgcagcg cctgcgcagc gccatctgcg agctgctgcc cgacgcagaa cagcaattgc
     3961 tgggcgaaca tgcacccgcc ctgctgctga ccaccgtgaa ctggaccacc tccaagctgc
     4021 agctgctggg cagccggccc acccttgggc agcgcgacca gttgggcctg acgctgatgg
     4081 acgccatgct ggccacctcg gcggcgccgg cgcattttcc ggtgcacagc ttccagcacc
     4141 accgcttcgt cgatggcggc ctggcggcca acgcgcccga cctgcacgcg ctgcaatgcg
     4201 cgcagcagat gtacccgggc gccgaagtgc acatgctcag cataggcacg gccaacccgc
     4261 tgcacggccg cgacccatcg gccgtgccgg ggcgcggcct gatgtgggcc ggcccggtga
     4321 tagaactcgt caccagcgcc caggagatgc tggccgtgca ggaatgcgcc gcccgcatgg
     4381 gaacgcaccg ctacctgcgg ctgaacatgc cgcccagcag cgcacagcaa aagcagctgg
     4441 actttgacgt ggccgaccag accagcaccg atctgctgct gtcgctggca gatgactgcc
     4501 tggcatcgtt gcagccggcc gactggcacc ggctcaaggc catcgtcggc tagccgggac
     4561 tcctgcgcgc tcaggggtgg gcgctggcgc tcttctccag ccgcacatcc tcgtacaaga
     4621 cctgcagcgg aagttgcagc cccacgctgg ccagttccac ctgctgcgcc gcggtgtcga
     4681 aggggtagag cacccagcgc ccggtttcgt cgcggcgaaa caggtccgcg ctcaggcgct
     4741 cgctgtcaat gagcaggtac tcactcagac tgggcagctt gcggttggcg gcaaactttg
     4801 cgccgcggtc ataggcgctg gtcgatggcg aaagcacctc cacgaccagg ctgggggcgc
     4861 ttttgtattg accctgctcg cggtcggcgg cggcccaggt gacgaacacg tcggggtaga
     4921 agaaggcgtt actttcctca gcgcagcttt atgtcagtga tgaactcgct gcaggggccg
     4981 ccacgcagat gctggcgcag ggccatggcg acattcaggg caatggtcac acgggcgtcc
     5041 gaggccccag ccacggcgaa cacctcgcca tccagatatt ggtgcttctc ggcctgaccc
     5101 tcttcccaat ccaggaaggt ctgggcatca cagcgcggct gggctgcagg caaggccatg
     5161 attggctcct cgatgtaccg cattgcagca cagcttcatt caggcgtaca ccacgggcgg
     5221 cgcatcggcg ccatcgggca gctccgtgca cccccagggt cgtcggaaaa ttttacacat
     5281 cgaccacgga aaattaaaaa cacccctata aatcaacgcg ttacatgaat ggcacaagaa
     5341 atgctttact tggtcgatct tattcacccg gatggttacc atgaaatctc tgctcatcgc
     5401 gaccctgtcc agcctctcct gtctggctgc gcttgccgcc cccatcacgc tgaccggtac
     5461 tgtgcgtgac ttcaatgctg acggggtgaa ttttgaaggc agcggcggct ctggcacggg
     5521 ctatgtcaac actaccctga gcggttcatc gccgaccctg acggcactgg gaagcagcca
     5581 gatcagcaac agcggccccg gggcattcag caattggtac accaagacca ccgacagcat
     5641 gtcttattcg ctgacgctga acgagacatc tccaggcagc ggcatctatc agtacagcaa
     5701 cagcaacttc ttccccatcg acggtgcact gctgggcaac cagggccgct cgcacaacta
     5761 ccactttacg tacaacatct cctcggtatt cagttatgtc caaggcgccg gccagacctt
     5821 cacgttcacc ggggatgatg acgtctgggt gtttttcgac cacaaactcg gcattgacct
     5881 gggaggtgtt catgccgccc aaagtcaaac ggtgaacctc gatacgttga tggccggcaa
     5941 gacctccggc gactacagct ttgatttctt cttcgccgaa cgacacacca cacaatcgaa
     6001 tctgatgatt acgacatcgc tggttttgaa gtcgcccccg accaacgtgc ccgaacctgg
     6061 ttcgttggcc ttgatgggtc tttccatggt tggcctggcc tttgtccgtc gccgcaagac
     6121 ggcttgattg attcctaccc caaccacgca gcggcgcctt tgggcgccgt tgtcgtatgc
     6181 gggcgcatcg ccattccgtt tccaaaacat tcgtgtccag gcgcgaagcc gcaggcagtg
     6241 ctgtcgcacg acaaggcgaa gcaacaatga cacgagtgat ttagagatgg agtcgcatct
     6301 gctacttggc accgctgcgc tatgccccgc acacgcgcag cacctcgtcc ccgtaggcct
     6361 ccagcttctt cgcccccatg ccgctgatgc cctccagctc gcccaggctg gccgggttgc
     6421 gttcggcaat cgccgccagc gtggcgtcgt ggaagatcac gtaggccggc aggttgtgcg
     6481 cgcgcgccac ctcggcgcgc caggccttga ggttgatgaa gcgcacctgg gcgtccgggc
     6541 ccaggtctgc ggcggcggcg ctcggctggc cggcgcggcg cttaggtttc gccgcggcct
     6601 tggcctgggc ctcgcgcagc tggacctgct gctcaccgcg cagcacggcg cgcgagccgg
     6661 gcgtgagcgt cagcgtgtcg aagctgtggc caccctcggt cgccacgcgg tgcacgccta
     6721 ccgcccccag cgccagcagc tggcgcagca cgccacgcag ctgttgctct gaatactgcg
     6781 cgcccaggtt gaaggtggac agctggtcgt ggccaaactg ccgcaccttg tccgtggcct
     6841 tgccgcgcac aatgtccatc aaatggccga cgccaaaggt caggcggctc acctcgtgca
     6901 cgcggtagat ggtggacagg agcttcctcg ccgcgtccgt gccgtcccat accgcgggcg
     6961 ggttcaggca gttgtcgcag ttgccgcaag gctgggattc ttcaccaaag taggccagca
     7021 gccgctggcg ccggcagtcc gtggcctcgg ccagggccag cagggcgtcg agcttgccgc
     7081 gcagcacggc cttgaactcc tcggccgcgt cgccgtcgtc gatcatgcgg cgctggttca
     7141 ccacgtcctg caggccgta
//
LOCUS       DAOMTQ010000149         7143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2413_length_7143_cov_25.519611, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000149 DAOMTQ010000000
VERSION     DAOMTQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_7143_cov_25.519611"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK518_12635"
     CDS             complement(<1..508)
                     /locus_tag="PK518_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HRM49801.1"
                     /translation="MNDKQPAPSTLPRDGVTPLNHLGVIRVAGEDAAKFLHGQLTQDF
                     ALLDEQHARLAALLTAKGRMLASCIAFKRSDAEVLLVCARDLLAPTLKRLSMFVLRAK
                     ARLSDASGDYALYGLLGDAARAALPEGDEPWAKADLGDASVVRLYPADGQPRALWVAP
                     AAVEAPAGP"
     gene            556..1545
                     /gene="mltG"
                     /locus_tag="PK518_12640"
     CDS             556..1545
                     /gene="mltG"
                     /locus_tag="PK518_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRM49802.1"
                     /translation="MRRFLVLLVLIAVIVGAAAAWWLQAPLTLAANQSLELEIEPGTT
                     PRAVARAVVQAGVQTDARLLYWWFRLSGKDRGIKAGNYEIPQGTSPYELLQKLVRGEE
                     ALRAVTLVEGWNFRQLRQALARAEQLKPETADWSDADIMARLGHAGLAAEGRFFPDTY
                     TYAKGSSDLAVLRRALHAMDRRLEAAWAQRAPDTPLKSADQALILASIVEKETGRAED
                     RAQIAGVFTNRLRVGMLLQTDPTVIYGLGEKFDGNLRRRDLQADTPYNTYTRAGLPPT
                     PIAMPGRAALLAAVQPAQTRALYFVARGDGSSHFSESLDEHNRAVNRYQRGQK"
     gene            1546..2199
                     /gene="tmk"
                     /locus_tag="PK518_12645"
     CDS             1546..2199
                     /gene="tmk"
                     /locus_tag="PK518_12645"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRM49803.1"
                     /translation="MQGLFITFEGIDGAGKSSHIDTLAQALRAAGRQVTLTREPGGTP
                     LAEKLRAMLLHDAMDALTETLLVFAARRDHLRVVIEPALARGDVVLCDRFTDATFAYQ
                     GVGRGFDLQVLSYMERLAQTGIALEPDLMRNPDLTLWFDVPPEVAAERLAAARTPDRF
                     EAEGVEFFTRVARGYADRAAGATQRFARLDGAQPRAKVAAQMLAAVAARGWLQGATP"
     gene            2196..3191
                     /locus_tag="PK518_12650"
     CDS             2196..3191
                     /locus_tag="PK518_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRM49804.1"
                     /translation="MTAVAPWIAAQRTQLLAQRGHAWLLQGPSGLGQYQLALELVRAW
                     LCEAPTEQGACGQCASCHGIAVHTHADLCVLMPETQMLALGWPLPEKAQAEIEDKKRK
                     PSREIRVEAMRDAVEFAQRTSGRGRGKAVLVFPAEQMNHVTANALLKTLEEPPGDVRF
                     VLATEAAHELLPTIRSRCLGHTMLWPRADVAQAWLVEQGVTPDAAQALLRAAGGRPDD
                     VLALHRAGRSPQAWALIPRAMARGEVAALADLEPAQAVDALQKLCHDLLASRVGAQPR
                     YFAAADLPQAPGVGPLTRWSRALMQEARTADHPFNAGLMLESLVAQAKSTLNSRR"
     gene            3208..3567
                     /locus_tag="PK518_12655"
     CDS             3208..3567
                     /locus_tag="PK518_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRM49805.1"
                     /translation="MSSPSTAPRPSVMQLNIKEKAALYAAYIPFFEEGGIFVPTPREY
                     KLGDDVYVLLTLPDDPQRYPVAGRVAWVTPARAAGNRTQGVGIQFPKDEKSRQLKHKI
                     EEILGTALASDKPTQTI"
     gene            3626..4435
                     /locus_tag="PK518_12660"
     CDS             3626..4435
                     /locus_tag="PK518_12660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRM49806.1"
                     /translation="MFVDSHCHLNFPELIDQLPQIRQAMAAAQVDRALCICTTMEEFA
                     AVHGLATRYDNFWATVGVHPDTEHMTEPSVQDLVERAALPRVVAIGETGLDYYGMEDR
                     KGGRSIADLDWQRERFRTHIRAARLTGKPLVIHTRSASEDTLAILREEGEDGGAGSAG
                     GVFHCFTESAQVARAALDMGFYVSFSGIITFKSAQDLRDVAAFVPMDRLLIETDSPYL
                     APVPYRGKTNNPSYVPFVARQIAAVKGLAVEQVAEATSRNFEQLFLQGAAA"
     gene            4432..5112
                     /locus_tag="PK518_12665"
     CDS             4432..5112
                     /locus_tag="PK518_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRM49807.1"
                     /translation="MTRRRTTLAWLAWAATVPVSGCAHAGAYEDFFKAIELDNGAAIS
                     DLLKRGFDPNARDPKGQPALVVALREDSRKAAAALIGARGLKVEERNAKDESALMLAA
                     LRGNLPAARALIKLDADVNKTGWTPLHYAASGTTQDAKAMVALLLEENAYIDAASPNG
                     TTPLMMAVRYGPGDVARLLVQEGADPTLKNELGLTALDFARQADRADMVELIAQAVRR
                     RQPSAGKW"
     gene            5291..5536
                     /locus_tag="PK518_12670"
     CDS             5291..5536
                     /locus_tag="PK518_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49808.1"
                     /translation="MEYSPSSSSCQHALLARSPLGHVTVCPDCAVVHLSLNCVSVRLE
                     VEAFAALAEMLNRAQECLHGSRLAQGLGHGGQACAVH"
     gene            5555..6361
                     /locus_tag="PK518_12675"
     CDS             5555..6361
                     /locus_tag="PK518_12675"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HRM49809.1"
                     /translation="MDRLQQQAGSALRADVAHVVGLAGEHGVLSRELGRVQERCSRVI
                     AEQSAEIERLKAQLLRQRAVAIAKDTRIAFLAQDLERLKASLPEYANTMKLREKLEQL
                     GARQADLDAQNARLRRQLAEAQHMLGQHATGGRPDPVAQHHGDAPRAIPVTVHSRKKT
                     ILCVGGRNGHMSSYRDAVEKAGGHFAHHDGALEDRQGTLDAVLAAADLVICQTACISH
                     NAYWRVKDFCQRTGKQCVFVENPSTSLLTRKLQQIAADEVAAKPVASSRW"
     gene            complement(6376..7053)
                     /locus_tag="PK518_12680"
     CDS             complement(6376..7053)
                     /locus_tag="PK518_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM49810.1"
                     /translation="MTSAADPQQNLSDRIAEQVREKIVHGEFAPGQRLSEAALSESLE
                     ISRNTLREAFRMLTKEGLLKHEPNRGVSVAVPSIAAIIDIYRVRRLVECQSLAQAYPR
                     HPARKQMRQAVDAALRWREAGDWRGVGTANMEFHKAIVALADSERLNDMFTHLLAELR
                     LAFGLLDDPEFLHAPYVDSNVKIIDLFEAGHPQEAAAALGDYLVHSERIVLAAYARRI
                     AGSGAGR"
BASE COUNT         1148 a         2416 c         2449 g         1130 t
ORIGIN      
        1 cggggcctgc cggggcctcg acagccgcag gggccaccca cagcgcgcgc ggctggccgt
       61 cggcggggta caggcgcacc acgctggcgt cgcccaggtc ggccttggcc cagggctcgt
      121 cgccctcggg cagcgcggcg cgcgcggcgt cacccagcag gccgtagagg gcgtagtcgc
      181 cgctggcatc gctcaggcgc gccttggcgc gcagcacgaa catggacagg cgcttgagcg
      241 tgggcgccag caggtcgcgt gcgcagacca gcagcacctc ggcatcgctg cgcttgaagg
      301 cgatgcagct ggcgagcatg cggcccttgg ccgtcagcag ggcggccaac cgtgcgtgct
      361 gctcgtccag cagggcaaag tcctgggtca gctggccgtg gaggaatttg gcggcgtctt
      421 caccggctac gcggatcacg ccaagatggt tcaggggggt cacgccgtcc cggggcaggg
      481 tggacggcgc tggctgcttg tcgttcatgc gctgaattat gatgcccggt tgtctctctt
      541 acgggctgca gggttgtgcg tcgttttctc gttttgctgg tgctcattgc cgtgatcgtt
      601 ggcgccgcgg ccgcgtggtg gctgcaggcg cccctgacac tggccgcgaa ccagtcgctg
      661 gagctggaga tagagcccgg caccacgccg cgcgccgtgg cccgcgccgt ggttcaggcc
      721 ggggtgcaga ccgatgcccg cctgctgtat tggtggtttc gcctgtcggg caaggatcgc
      781 ggcatcaagg ccggcaatta cgaaatcccc cagggcacca gcccctatga gttgctgcag
      841 aaactggtgc gaggcgagga agcgctgcgc gccgtgaccc tggtggaggg ctggaacttc
      901 cgccagctgc gccaggccct ggcacgcgcc gagcagctca aacccgagac cgcggattgg
      961 agcgatgccg acatcatggc ccgccttggg catgctggct tggccgcaga gggccgtttc
     1021 tttcccgata cctacaccta cgccaagggc agcagcgatc tggccgtgct ccgccgcgcc
     1081 ctgcatgcca tggacagacg tctggaagcc gcctgggcgc agcgcgcgcc ggatacgccg
     1141 ctcaagtccg ccgaccaggc gctgatcctg gccagcattg tcgagaagga aactggccgt
     1201 gccgaggacc gcgcccagat cgccggcgtg ttcaccaacc gcctgcgtgt gggcatgctg
     1261 ctgcagaccg accccacggt gatctacggc ctgggcgaaa aatttgacgg caacctgcgc
     1321 cggcgcgacc tgcaggccga cacaccctac aacacctaca cccgcgccgg cctgccgccc
     1381 accccgatcg ccatgccggg ccgcgccgcg ctgctggccg ccgtgcagcc ggcgcaaacc
     1441 agggcgctgt actttgtggc gcgcggcgac ggttccagtc acttcagcga atcgctggac
     1501 gagcacaacc gcgccgtcaa tcgctaccaa cgcgggcaga aataaatgca aggcctgttc
     1561 atcaccttcg agggcataga cggcgcgggc aagtcctcgc atatcgacac cctggcacag
     1621 gccctgcgcg ccgccgggcg ccaagtgacg ctcacgcgtg aaccgggtgg cacgccgctg
     1681 gccgagaagc tgcgcgccat gctgctgcac gatgccatgg acgcgttgac cgagacgctg
     1741 ctggtgtttg ccgcgcggcg cgaccatctg cgcgtggtga tagagccggc gctggcgcgc
     1801 ggcgacgtgg tgctgtgcga ccgcttcacc gacgccacct ttgcctacca gggggtaggg
     1861 cgcggctttg atctgcaggt gctctcttat atggagcgcc ttgcgcagac gggtattgcc
     1921 ctggagcccg atttgatgcg aaacccggat ctgaccctgt ggttcgacgt accgcccgag
     1981 gtggcggccg agcgcctggc cgccgcgcgc acccccgatc gcttcgaggc cgagggcgtg
     2041 gagtttttca cccgcgtggc gcgcggctat gccgatcgtg ccgccggcgc cacgcagcgc
     2101 tttgcccgcc tggacggcgc ccagccgcgc gcgaaggtcg ccgcccagat gctggccgcc
     2161 gtggccgcgc gtggttggct gcagggggcc acgccatgac ggcggtcgcg ccctggattg
     2221 ccgcgcagcg cacacagctc ttggcccagc gcggccatgc ctggctgctg caggggccct
     2281 cgggcctggg ccagtaccag ctggcgctgg agctggtgcg cgcctggctg tgcgaggcgc
     2341 ccaccgagca gggcgcctgt ggccagtgcg ccagctgcca cggcatagcg gtgcacaccc
     2401 atgccgacct gtgcgtgctc atgcccgaga cgcagatgct ggccctgggc tggcccctgc
     2461 ccgagaaggc ccaggccgag atcgaggaca agaagcgcaa gcccagccgc gagatccgtg
     2521 tcgaggccat gcgcgacgcc gtcgagtttg cccagcgcac cagcgggcgc gggcgcggca
     2581 aggcggtgct ggtgtttccg gccgagcaga tgaaccatgt cacggccaac gcgctgctga
     2641 agacgctgga ggagccgccc ggtgacgtgc gctttgtgct cgccaccgag gccgcgcacg
     2701 agctgctgcc caccatacgc agccgctgcc tgggccacac catgctctgg ccccgggccg
     2761 acgtggcgca ggcctggctg gtggagcagg gcgtgacgcc ggatgcggct caggcactgc
     2821 tgcgcgccgc cggtggccgg cccgacgacg tgctggccct gcaccgcgcc ggccgcagcc
     2881 cccaggcctg ggcgctgatc cccagggcca tggcgcgcgg tgaggtggcg gccctggccg
     2941 acctggaacc ggcccaggcc gtggatgcgc tgcaaaagct ctgccacgac ctgctggcca
     3001 gccgcgtggg cgcacagccg cgctactttg ccgcagccga cctgccgcag gcgccgggcg
     3061 tgggcccact cacgcgctgg tcgcgcgccc tgatgcagga ggcgcgcacg gccgatcacc
     3121 ccttcaacgc cggcctgatg ctggagtcgc ttgtagcgca ggcgaagagc acactaaact
     3181 ctcgccgttg aacgaccaaa gccacccatg agcagtccct ccacagcgcc gcgccccagc
     3241 gtcatgcagc tgaacatcaa ggaaaaggcc gcgctgtacg cggcctacat cccgttcttc
     3301 gaggaaggcg gcatcttcgt gccgacgccg cgcgagtaca agctgggcga cgatgtctac
     3361 gtgctgctga cgctacccga tgacccgcag cgctaccccg tggccggccg tgtggcctgg
     3421 gtgacccccg cacgcgcggc cggcaaccgc acgcagggcg tgggcatcca gttccccaag
     3481 gacgaaaaat cgcgccagct caagcacaag atcgaggaaa tcctgggcac cgccctggcc
     3541 tcggacaagc ccacgcagac catttgagct ttacccgctc aaccgccgac cgcgcgccgg
     3601 tcggcgtttt tgtttcttta ccaccatgtt tgtcgattct cactgtcacc tgaactttcc
     3661 cgagctgatc gaccagctgc cgcagatccg ccaggccatg gctgcggccc aggttgatcg
     3721 cgcgctgtgc atttgcacca cgatggagga atttgccgcc gtgcatggcc tggccacccg
     3781 ctacgacaat ttctgggcca ccgtgggcgt gcatcccgac accgagcaca tgacggagcc
     3841 gtccgtgcaa gacctggtcg agcgcgccgc gctgccgcgc gtggtggcca ttggcgagac
     3901 cgggctggac tactacggta tggaggaccg caagggcgga cgcagcattg ccgacctgga
     3961 ttggcagcgc gagcgttttc gcacccatat ccgcgcggcg cggttgacgg gcaagccctt
     4021 ggtgatccac acccgcagcg catccgagga cacgttggcc atcctgcgcg aggaaggcga
     4081 ggacggcggc gcgggcagcg ccggcggggt attccactgc ttcaccgaga gcgcccaggt
     4141 ggcgcgcgcg gcgctggaca tgggtttcta cgtctccttc tcgggcatca tcaccttcaa
     4201 gagcgcgcaa gacctgcgcg atgtggcggc cttcgtgccc atggacaggc tgctgatcga
     4261 aaccgacagc ccctacctgg cgcccgtgcc ctaccgcggc aagaccaaca acccgtccta
     4321 cgtgcccttc gtggcccggc agatcgcggc cgtgaagggg ctggccgtgg agcaggtggc
     4381 cgaggccacc agccgcaatt ttgaacagct gttcttgcaa ggagccgccg catgacgcgc
     4441 cgccgcacca ccctggcctg gctggcatgg gccgccacgg ttcccgtctc gggctgcgcc
     4501 catgcgggtg cctacgagga tttcttcaag gccatagagc tggacaacgg cgccgccatc
     4561 agcgatctgc tcaaacgcgg cttcgacccg aatgcgcgtg accccaaggg ccagccggcg
     4621 ctggtcgtgg cgctgcgcga ggattcgcgc aaggccgccg cggccctgat cggcgccagg
     4681 ggcctgaagg tggaggagcg caacgccaag gacgaaagcg ccctgatgct ggccgcgctg
     4741 cgcggcaacc tgccggcggc gcgcgccctg atcaaactcg acgccgacgt caacaagacc
     4801 ggctggacgc cgctgcacta cgccgcctcg ggcaccacgc aggacgccaa ggccatggtg
     4861 gcgctgctgc tggaagaaaa cgcctacatt gacgccgcct cgcccaacgg caccacgccg
     4921 ttgatgatgg ccgtgcgcta cggcccgggc gatgtggcgc ggctcttggt gcaggagggc
     4981 gccgacccga cgctgaagaa cgaactgggc ctgacggcgc tggactttgc ccgccaggcc
     5041 gaccgcgccg acatggtgga gctgatcgcc caggccgtgc gccggcgcca gcccagtgca
     5101 ggcaaatggt aatgctataa aaataattgc tatctgcgct tgatacacaa gcgttaaggc
     5161 ggtattttgc tgttgaattg gtgatggaga actcctgcga aagcggctgg catggggttc
     5221 acgaagattc ttttaaatgc gaacgatttg catttagaat gaattctcca accacacacc
     5281 ggaggatcgc atggagtact cgccttcttc atccagctgc cagcatgcct tgctggcgcg
     5341 cagcccgctc ggtcatgtga ccgtctgccc ggattgcgcc gtggtgcatc tgtcgctcaa
     5401 ctgcgtctcc gtgcggctgg aggtggaggc cttcgccgcc ctggccgaga tgctgaatcg
     5461 ggcccaggaa tgcctgcatg gcagccggct agcgcagggc ctcggccatg gcggccaggc
     5521 gtgcgcggtg cactgaagag cgggtctgcg caccatggac aggctgcagc aacaggccgg
     5581 gtcggcgctg cgggccgacg ttgcccatgt cgttggtctg gccggcgagc atggggtctt
     5641 gtcccgcgag ctgggcaggg tgcaggagcg ctgctcccgg gtcatcgccg agcagtcggc
     5701 cgagatcgaa cggctcaagg cccagctgct gcgccagcgc gccgtggcga tcgccaagga
     5761 cacgcggatt gccttcctgg cgcaggatct ggagcggctc aaggcgtcgc tgccggagta
     5821 tgcaaacacc atgaaactgc gcgagaagct ggagcagctc ggcgcccggc aggccgatct
     5881 ggacgcgcag aatgcaaggc tgcgccggca gctggctgaa gcgcagcata tgctgggcca
     5941 gcatgcgacc gggggtaggc ccgatccggt cgcgcagcat cacggcgacg cgcccagggc
     6001 catcccggtg accgttcact caaggaaaaa aaccattttg tgcgtgggtg gccgcaacgg
     6061 ccatatgtcc agctaccggg atgccgtgga gaaggcaggc ggccattttg cgcaccacga
     6121 cggcgctctg gaggataggc aagggacgct ggacgcggtg cttgccgcgg ccgatctggt
     6181 catctgccaa accgcctgca tcagccacaa cgcctattgg cgcgtcaagg atttttgcca
     6241 gcgcaccggc aagcaatgcg tgttcgtgga gaaccccagc acctctttgt tgacccgcaa
     6301 gctgcaacaa atcgccgccg atgaggttgc tgcaaagccg gttgccagct cccgctggtg
     6361 accggcctga gggcctcacc tgcccgcgcc gctgcctgca atgcgccgcg cataggccgc
     6421 cagcacgatg cgctcggagt gcaccaggta gtcgcccagc gcggccgcag cctcttgggg
     6481 gtggccggct tcgaacaggt cgatgatctt cacgttgctg tccacatagg gtgcgtgcag
     6541 aaactccggg tcgtccagca gcccgaaggc caggcgcagc tcggccagca ggtgggtgaa
     6601 catgtcgttc agccgctcgc tgtcggccag cgccacgatg gccttgtgga actccatgtt
     6661 ggccgtgccc acgccgcgcc agtcgccggc ctcgcgccag cgcagggccg cgtccacggc
     6721 ctggcgcatc tgcttgcggg cggggtggcg tgggtaggcc tgggccaggg actggcattc
     6781 aacgagccgg cgcacgcggt agatgtcgat gatggccgcg atgctgggca cggccaccga
     6841 cacgccacgg ttgggctcgt gtttcagcag tccttccttg gtcagcatgc ggaaggcctc
     6901 gcgcagcgtg ttgcgcgaga tttccaggct ctcgctcagt gccgcttcgg acaggcgctg
     6961 gcccggggcg aactcaccat gcacgatttt ctcgcgcacc tgctccgcaa tgcggtcgga
     7021 gaggttttgt tgcgggtcgg cggcactcgt catgggctgg gcgggctggt gttgaggcag
     7081 caagtgtagc cagcacccgc atgcccgttt gccgcatggc ggcagcgctg cgggggtgat
     7141 ctc
//
LOCUS       DAOMTQ010000150         7130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2422_length_7130_cov_24.390530, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000150 DAOMTQ010000000
VERSION     DAOMTQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_7130_cov_24.390530"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK518_12685"
     CDS             complement(<1..206)
                     /locus_tag="PK518_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRM49811.1"
                     /translation="MKPHKNLELWQQAMVLVQMVYEATSGFPREELFGLTNQMRRAAV
                     SVPSNIAEGAGRGGEKEFLHFLNIA"
     gene            complement(250..1152)
                     /gene="rfbD"
                     /locus_tag="PK518_12690"
                     /pseudo
     CDS             complement(250..1152)
                     /gene="rfbD"
                     /locus_tag="PK518_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1234..2289)
                     /gene="rfbB"
                     /locus_tag="PK518_12695"
     CDS             complement(1234..2289)
                     /gene="rfbB"
                     /locus_tag="PK518_12695"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRM49812.1"
                     /translation="MILVTGGAGFIGANFVLDWLGGCDEPVLNLDKLTYAGNLHNLAS
                     LEGDARHVFVQGDIGDRALVESLLARHQPRAIVNFAAESHVDRSIHGPEDFIQTNVLG
                     SFRLLEAVRHYWKALPAAEKEAFRFLHVSTDEVYGSLAPSAPAFTEEHNYEPNSPYSA
                     SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPLHFPEKLIPLMIVNALAGKNLPVYGDG
                     MQVRDWLYVRDHCSAIRRVLEAGKVGETYNVGGWNEKPNIEIVKTVCALLDELRPRAD
                     GESYASQISYVTDRPGHDRRYAIDARKLERELAWKPAETFDTGIRKTVEWYLANPDWV
                     AQVQSGAYREWVAKQYA"
     gene            2458..2901
                     /locus_tag="PK518_12700"
     CDS             2458..2901
                     /locus_tag="PK518_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRM49813.1"
                     /translation="MARAPEDDDTSNPNPAHDLPRMARESAQQVWLAGLGAFAKAQQE
                     GGKVFEALVREGQALQRKTQDAAGEQLSEAAQRMGHLASGLGERAAGQWDRLEGIFEE
                     RVAKALQRLGVPTAQEVQALRERVAALEEALDDALAGRHDKKPGD"
     gene            2996..3688
                     /locus_tag="PK518_12705"
     CDS             2996..3688
                     /locus_tag="PK518_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM49814.1"
                     /translation="MARKAPRRTAERILEASLALFNRFGEPHVSTAAIAADLGISSGN
                     LYYHYRAKDEIVNALFAQYEQALGELLQAGGDVADVEDAWFFTHRLFELVWQYRFLYR
                     DLNNLLSRNRQLETQFPALLRAKTQAMHALLAALGRSGALRLPPDGAAQTTANCMVLV
                     ATYWLSFEYVHDPRRALEPEGAQAALLGGARHALGLLLPYLEPAQCAHLARLAGAYGA
                     AAHPAPALDDGE"
     gene            complement(3690..4466)
                     /locus_tag="PK518_12710"
     CDS             complement(3690..4466)
                     /locus_tag="PK518_12710"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRM49815.1"
                     /translation="MTFLAIDVGNSRLKWALYAAARPGAALLAHGVEFLDHIERLADG
                     QWADLPAPTHMLGCVVAGDAVKRRVAEQMDLWDVPARWVVPSAEEAGVVNGYDHPSRL
                     GADRWVAMIGARHHLLAQGPARPLVLAMVGTAVTVEALDAEGRFLGGLILPGHGIMLR
                     ALETGTAGLHVPTGEVRPFPTNTSDALTSGGTYAIAGAVERLYQHLQQHCGQEPLCLM
                     TGGAGWKMAPSMARPFELVENLIFDGLLEIGARRFAPAAA"
     gene            4607..5095
                     /gene="lysM"
                     /locus_tag="PK518_12715"
     CDS             4607..5095
                     /gene="lysM"
                     /locus_tag="PK518_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HRM49816.1"
                     /translation="MGLFTFIKEAGEKLFGGKDAHAASPTAPTPAELNAQAGKAIGNY
                     IAAQNLGVSDVQVTFDGAEGKVTVAGVAPTQAVKEKVTLCCGNVASVTSVDNQMSVTN
                     PEPEAQYHDVVRGDTLSAIAKKFYGDANKYPVIFEANKPMLSHPDKIYPGQKLRIPAL
                     AR"
     gene            complement(5397..5822)
                     /locus_tag="PK518_12720"
     CDS             complement(5397..5822)
                     /locus_tag="PK518_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM49817.1"
                     /translation="MNQAAIDQPRPADGYAGDISPQLAWQWASSGQAVLVDVRSDAER
                     DWVGHVPGALAVVWKQWPGMRLNIDFDAQLCAAVPPGTKVLLLCRSGVRSIAAARRAT
                     ELGLQAYNILEGFEGDPDANGQRNRLGGWRFHGLPWRQG"
     gene            complement(5834..6649)
                     /locus_tag="PK518_12725"
     CDS             complement(5834..6649)
                     /locus_tag="PK518_12725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRM49818.1"
                     /translation="MTMLLQGLLDLLGGAGDAPPAKTPAPTRPERPRQVLLAGQPVPY
                     SLQRARRRSIGFSVGPDGLAVRAPAWVTLAEVDAALQSKADWIVRKLAEQRLRREQQE
                     ATRIAWGHGARLPYLGQPLTVLLAGRQPARASLVPAQDGAHALHLPLAQGTAPAEVRA
                     AVQAWLMREARRRFTERLDHFAPLLGVHWTRLRLSSAATRWGSARTGGAISLNWRLMH
                     YPPPIIDYVVAHELAHLRVMDHSPRFWSVVHSVVPNHAQLRRQLREQPAPLWE"
     gene            complement(6646..>7130)
                     /locus_tag="PK518_12730"
     CDS             complement(6646..>7130)
                     /locus_tag="PK518_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM49819.1"
                     /translation="MPAIMPHALAYVFKKELLRIPFFGWSIGSLDMIHIDRSQKARAF
                     VHVIKRGKQLLGQGVWVIMFPEGTRTARGEKGTYQASGARLAIEAGVPVVPVAVTSAR
                     CWPRKAWVKTPGVVDVSIGRPMATAGRKSDELMREVEDWIESEMRRLDPDAYAPARDA
                     "
BASE COUNT         1163 a         2357 c         2452 g         1158 t
ORIGIN      
        1 gcaatgttca gaaaatgcaa aaactccttc tcgccaccac gcccagcgcc ttcggcaata
       61 ttggatggca cggaaacggc cgccctgcgc atctggttgg tcaagccaaa caactcttcg
      121 cgcgggaaac cagaagtcgc ctcgtagacc atttgcacca acaccatcgc ctgctgccac
      181 agctcaagat tcttgtgagg tttcattgtt tggtaagtgg ttggcaacgg gtcactcacc
      241 actcacgatt caccacttac caacctcctc cagcatgcgc gccacgccat gctgccaggg
      301 cggcagggtc aggccgaagg tggcttgcag tctggatgtg ttcaggcgcg agttgagcgg
      361 gcgccaggcg ggggtgggaa aggcactggt aggcacgggg ctgatctctt ttgctcttat
      421 ttgtatagct ttttgcgctt gactggcttg gtctatgacg tattttgcat aggaatgcca
      481 ggtggtctcg ccggcggcgg cgcagtggta caggccgccg tcctgcgggc gtgcctgcag
      541 ctggcgcagg gcgtgggccg tgacgtcggc cagcagatcg gcgccggtgg gcgcgcccca
      601 ttggtcgttg atcaccgtga gatgttcacg ctcctgggcc aggcgcagca tggtcttggc
      661 gaagttgccg ccgcgcgcgg cgtagaccca gctggtgcgc aatatcaggt gctggcagcc
      721 cgcttgctgg atgagctgct caccctccag cttggtggcg ccgtagaccg agagcggacc
      781 ggtggcgtcg tcctcggtcc agggccggtc gccgctgccg tcaaacacat agtcggtgct
      841 gtagtgcacc agccaggcgc ccaggcgcgc ggcctcttgc gccagcgcgc cgggcgtggt
      901 ggcgttgagc aggcgcgcgc gcgcgggctc gctctctgcc ttgtctacgg cggtatgtgc
      961 tgcggcgttg acgatgacgt cgggacacag ggcgcgcacg gtgtcggcca cggcatcggg
     1021 ctgggcaaag tcgccgcaca gctgggtgct gtcatggctc agcgcggtca cctggcccag
     1081 cacggacagg ctgcgctgca gctcccagcc gagctggccg ttcttgccaa agagcaggat
     1141 gttcatgggg gcagatggta agtagtgagt ggtaaatggt aagtggcgcg ctggttttca
     1201 ccatttacca ctcaccattc acgacttacc agattaagcg tactgcttgg ccacccattc
     1261 gcggtaggcg ccgctttgca cctgggccac ccagtccggg ttggccaggt accactcaac
     1321 ggtcttgcgg atgccggtgt cgaaggtttc ggcgggcttc caggccaatt cgcgctcgag
     1381 cttgcgcgcg tctatggcgt agcggcggtc gtggccgggg cggtctgtca cgtagctgat
     1441 ctggctggcg tagctttcgc catcggcgcg cgggcgcagt tcgtcgagca gggcgcagac
     1501 ggttttcacg atctcgatgt tgggcttttc gttccagccg ccgacgttgt aggtctcgcc
     1561 caccttgccg gcctccagca cgcggcggat ggcgctgcag tggtcgcgca cgtagagcca
     1621 gtcgcgcacc tgcatgccgt cgccgtagac gggcaggttt ttgcctgcca gggcgttgac
     1681 gatcatcagc gggatgagct tttccgggaa gtgcagcggg ccatagttgt tgctgcaatt
     1741 cgtcgtgagc accggcaggc cgtaggtgtg gtgccaggcg cgcaccaggt ggtcgctggc
     1801 ggccttgctg gcgctgtacg ggctgttggg ctcgtagttg tgttcttcgg tgaaggccgg
     1861 ggcgctgggg gccaggctgc cgtagacctc gtcggtgctc acatgcagaa agcgaaacgc
     1921 ttctttttct gcagctggca gcgctttcca gtagtgcctt acagcctcca aaagcctgaa
     1981 gctgccgagc acgttggtct ggatgaagtc ctcggggccg tggatggagc ggtccacatg
     2041 gctttcggcg gcaaagttga caatggcgcg cggctggtgg cgggccagca ggctttccac
     2101 cagggcccgg tcgccgatgt cgccctgcac gaagacatgg cgcgcgtcgc cttccaggct
     2161 ggccaggttg tgcaggttgc cggcgtaggt cagcttatcc aggttgagca cgggctcgtc
     2221 acagccgcca agccagtcga gtacgaagtt ggcgccaatg aagccggcgc cgccggtgac
     2281 caggatcatg gggaaaatct ctcgcgcaaa aggtaagcgc cgcaggggca aagtcaggga
     2341 ttatcccatt gacccgttga ctgacgtaac cagccgtcat gtatggatta gggccatctg
     2401 cctataccca tgccgcggca gttatccaca atggccgcat cacaccaagg aagcagcatg
     2461 gcccgcgcac ctgaagacga cgacacctcc aaccccaacc ccgcccatga cctgccgcgc
     2521 atggcgcgcg agtcggcgca gcaggtctgg ctggccgggc tgggcgcgtt tgccaaggcg
     2581 cagcaggagg gcggcaaggt cttcgaggcc ctggtgcgcg agggccaggc gctgcagcgc
     2641 aagacccagg acgccgccgg cgagcagctg agcgaggccg cccagcgcat gggccacctg
     2701 gcatcgggcc tgggcgagcg cgcggccggc cagtgggaca ggctggaggg catcttcgag
     2761 gagcgcgtcg ccaaggctct gcagcgcctg ggcgtgccca cggcgcagga agtacaggcg
     2821 ctgcgcgagc gcgtggcggc gctggaagag gcgctggatg acgccctggc cgggcggcac
     2881 gacaaaaagc ccggggattg acggccacgc gcccgcaacg ggctgcaggc tgacaccgat
     2941 ttgacccact tgcggcgtac agtgccatgg tctccacgac catcggagga cggccatggc
     3001 ccgcaaagcc ccacgccgca cggcagagcg catcctcgaa gccagcctgg cgctgttcaa
     3061 ccgctttggc gagccccatg tctccaccgc cgccattgcc gccgacctgg gcatcagctc
     3121 gggcaacctg tactaccact accgcgccaa ggacgagatc gtcaacgcgc tgttcgcgca
     3181 gtacgaacag gcgctgggcg agctcttgca ggccggtggc gacgtggccg atgtggagga
     3241 cgcctggttc ttcacgcacc gcctgttcga gctggtctgg caataccgct ttctgtaccg
     3301 cgacctgaac aacctgctgt cgcgcaaccg ccagctggag acgcagttcc cggccctgct
     3361 gcgcgccaag acccaggcca tgcacgcact gctggccgcc ctgggccgca gcggcgcgct
     3421 gcgcctgcca ccggacggcg cggcgcagac cacggccaac tgcatggtgc tggtggccac
     3481 ctactggctg agctttgaat atgtgcacga cccgcgccgc gcgctggagc ccgaaggcgc
     3541 ccaggccgcg ctgctgggcg gcgcgcgcca tgcgctgggc ttgctgctgc cctacctgga
     3601 gccggcgcaa tgcgcgcatc tggcgcggct ggcgggggcc tatggcgcgg cggcgcaccc
     3661 tgccccagcc ctggacgacg gggaatagct caggctgcgg ccggcgcaaa gcggcgcgcg
     3721 ccgatctcca gcaggccgtc gaagatcagg ttttccacca gctcgaaggg gcgcgccatg
     3781 ctgggcgcca tcttccagcc ggcgccgccg gtcatcaggc acaggggctc ctggccgcaa
     3841 tgctgctgca ggtgctggta caggcgctcc acggcgccgg cgatggcgta ggtgccgccg
     3901 ctggtgagcg cgtcactggt gttggtggga aacggccgca cctcgcccgt gggcacatgc
     3961 aggccggcgg tgccggtctc cagcgcacgc agcatgatgc cgtggccggg caggatcagg
     4021 ccgcccagaa agcggccctc ggcgtccagc gcctccaccg tcaccgccgt gcccaccatg
     4081 gccagcacca gcggacgcgc cgggccctgg gccagcagat ggtggcgcgc gccaatcatg
     4141 gccacccagc gatcggcgcc caggcgcgag gggtggtcgt agccgttgac cacgccggcc
     4201 tcctcggccg agggcacgac ccagcgcgcg ggcacgtccc acaggtccat ctgctcggcc
     4261 acgcggcgct tgacggcgtc acccgccacc acgcagccca gcatgtgcgt gggcgcgggc
     4321 agatcggccc actggccgtc ggccaggcgc tcgatgtggt cgagaaactc cacgccatgg
     4381 gcgagcagcg cggcgccggg ccgcgccgcc gcgtacagcg cccatttcag gcgggaattg
     4441 cccacgtcga tggccagaaa tgtcatgggg cgcgattatc caaaagtttt gcggcgcacg
     4501 gcgctgcagg cagcggaatg cgaagaattg tcaaagcgcg gtttatacgc ccatctgccg
     4561 ttacattcat ccgtcttgat ttgattgcaa ccaataggag agaaacatgg gcctgttcac
     4621 attcatcaag gaagccggtg aaaagctgtt tggcggcaag gacgcgcatg cagccagccc
     4681 cacggcgccc acgccggccg agctcaacgc ccaggccggc aaggccattg gcaactacat
     4741 cgccgcgcag aacctgggcg tgagcgatgt gcaggtcacg ttcgacggcg ccgagggcaa
     4801 ggtcacggtg gctggcgtgg cccccacaca ggccgtcaag gagaaggtga ccctgtgctg
     4861 cggcaacgtg gccagcgtga ccagcgtgga caaccagatg tcggtcacca accccgagcc
     4921 cgaggcccag taccacgacg tggtgcgcgg cgacacgctg agcgcgattg ccaagaagtt
     4981 ctatggcgac gccaacaaat acccggtgat cttcgaggcc aacaagccca tgctgagcca
     5041 cccggacaag atctacccag gccagaagct gcgcattccg gcattggcgc gctaagcttc
     5101 attcagcatc aaaatagctg ccagcgcttg tgtagcaagc gctggcagct attgttttta
     5161 ttgatggtga gcaacgcttg tttagcgcgg tttttaaccc cctcgccccc ttgggggaga
     5221 gggttggggt gagggggtga tgccgcgcaa cacccggttt tttgcaccct cacccctacc
     5281 ctctcccgca cgcgggagag ggagaaacag ccctcaaggc gcaatcacac tgcgcggtgc
     5341 gctatcaaaa aaagtatgca tccacaaagc cagagggggc gcagggtggg ccatcgtcac
     5401 ccctgccgcc acggcaggcc atgaaagcgc cagccgccca ggcggttgcg ctggccattc
     5461 gcgtcggggt cgccctcgaa gccttccagg atgttgtagg cctgcaggcc cagctcggtg
     5521 gcgcggcgcg ccgccgcgat ggagcgcacg ccgctgcggc acagcagcag gaccttggtt
     5581 cccggcggca cggcggcgca tagctgcgcg tcgaagtcga tgttcaggcg catgccgggc
     5641 cattgcttcc agaccacggc cagggcgccg ggcacatggc ccacccagtc gcgctcggcg
     5701 tccgagcgca cgtccaccaa caccgcctgg ccgctgctgg cccattgcca ggccagttgc
     5761 gggctgatgt cgccggcgta gccatcggcg ggccggggtt ggtcgattgc ggcttggttc
     5821 atgttcaaca gtcctattcc cacagcggcg ccggctgctc gcgcagctgg cgccgcagct
     5881 gggcatggtt gggcaccacg ctgtgcacca cgctccagaa acgcgggctg tggtccatca
     5941 cgcgcaggtg cgccagctca tgcgccacca cgtagtcgat gatgggcggc gggtaatgca
     6001 tcaggcgcca gttcaggctg atggcgccgc cggtgcgggc gctgccccag cgcgtggcgg
     6061 cgctggacag gcgcaggcgc gtccagtgca cgcccagcag gggggcgaaa tggtcgagcc
     6121 gctcggtaaa gcgccggcgc gcctcgcgca tcagccaggc ctgcacggcg gcgcgcacct
     6181 cggccggcgc cgtgccctgg gccagcggca ggtgcagcgc atgcgcgccg tcctgtgccg
     6241 gcaccaggct ggcgcgcgcc ggctggcggc cggccagcag cacggtgagc ggctggccca
     6301 ggtagggcag gcgtgcacca tggccccagg cgatgcgcgt ggcctcttgc tgctcgcggc
     6361 gcaggcgctg ctcggccagc ttgcgcacaa tccagtcggc cttggactgc aacgcggcat
     6421 cgacctccgc cagcgtgacc caggcgggcg cgcgcaccgc cagaccgtcg ggcccgacgc
     6481 tgaagcctat ggagcgccgg cgcgcgcgct gcaggctgta gggcaccggc tgcccggcca
     6541 gcagcacctg gcgcggccgc tctggcctgg ttggcgcggg cgttttggcg ggcggcgcat
     6601 cgcccgcgcc gcccagcaga tcgagcaggc cttgcagcag catcgtcagg cgtcccgggc
     6661 cggggcgtag gcgtcggggt ccaggcggcg catctcggac tctatccagt cctcgacctc
     6721 gcgcatcagc tcgtcggact tgcgcccggc cgtggccatg ggccggccta tggagacatc
     6781 gaccacgccc ggggtcttca cccaagcctt gcgcggccag cagcgcgccg acgtgacggc
     6841 cacgggcacg acgggcacgc cggcctcgat cgccaggcgc gcgccgctgg cctggtaggt
     6901 gcccttttcg ccgcgcgccg tgcgcgtgcc ctcggggaac atgatgaccc acacgccctg
     6961 gcccagcagc tgcttgccgc gcttgatgac atgcacgaag gcgcgcgcct tctggctgcg
     7021 gtcgatatgg atcatgtcca ggctgccaat cgaccagccg aagaacggga tgcgcagcag
     7081 ctccttcttg aacacatagg ccagggcgtg gggcatgatg gccggcatca
//
LOCUS       DAOMTQ010000151         7127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2424_length_7127_cov_26.860436, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000151 DAOMTQ010000000
VERSION     DAOMTQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_7127_cov_26.860436"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK518_12735"
     CDS             complement(<1..662)
                     /locus_tag="PK518_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRM49820.1"
                     /translation="MSYTKKDIAVRKFDVVIVGAGGSGMRASLELSRAGLSVACLTKV
                     FPTRSHTVAAQGGVSASLGNMSEDNWHYHFYDTIKGGDWLSDQDAVEFMCREAPNVVI
                     ELEHFGMPFDRNADGTIYQRPFGGHTANYGEKPVQRACAAADRTGHAMLHTLYQKNVE
                     SKTNFFVEWMALDLIRNSTGDVVGVTALELETGDLYELHAKAVLLATGGAGRIFAAST
                     NA"
     gene            complement(726..1091)
                     /gene="sdhD"
                     /locus_tag="PK518_12740"
     CDS             complement(726..1091)
                     /gene="sdhD"
                     /locus_tag="PK518_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HRM49821.1"
                     /translation="MAVTYGSKRTVVGAHYGWRDFVVQRITAILLLAFTLLLLAQLLF
                     TKGPIGYDTWAGIFSPQWMKALTFSVIVALIWHAWVGVCSVWYDYAKPAGLRLVLQAL
                     SAIWLVSCGGWAVQVLWRL"
     gene            complement(1116..1550)
                     /gene="sdhC"
                     /locus_tag="PK518_12745"
     CDS             complement(1116..1550)
                     /gene="sdhC"
                     /locus_tag="PK518_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRM49822.1"
                     /translation="MTELAKKKRPEFRNLNLFSDVRTYRLPLAGFISILHRVSGMLMF
                     ILLPLVIWMFDTSVSSEISFGRFSAAFNVGLGFVPGWFIKLVTLALIWAYLHHLAAGV
                     RHLYLDVSHKTTKDFGRQSAVATLVFSIGLTLILGAKLFGLY"
     gene            complement(1712..2503)
                     /locus_tag="PK518_12750"
     CDS             complement(1712..2503)
                     /locus_tag="PK518_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM49823.1"
                     /translation="MSSLLPPSTEGTPPASGGAATVTPAFSPLYQQIKGLILHSLQHG
                     EWKPGEAIPSEIELAARFRVSQGTVRKAIDELAAENLVTRRQGKGTFVATHAERQVQY
                     RFLKLLPDAGDASEEGPAQRTILECRRLRASADMARSLALRAGDSVIQIKRILSFVGV
                     PTILEDIWLPGQAFKGLTAEQLAHYQGPTYAMFELDFGVRMVRAEEKIRAVLPDETQA
                     QWLNVPAGTPLLSVERLAYTYNDVPMELRRGLYRTDTHHYHNKLN"
     gene            2701..3687
                     /locus_tag="PK518_12755"
     CDS             2701..3687
                     /locus_tag="PK518_12755"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRM49824.1"
                     /translation="MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQNALKGVIMELEDCAFPLLAGIEAHSDPMTAFKDTDYALLVGARPRGPGMERA
                     DLLAANAQIFTAQGKALNAVASRNVRVLVVGNPANTNAYIAMKSAPDLPRENFTAMLR
                     LDHNRAASQIATKMGVAVGDIEKLAVWGNHSPTMYADYRFATVGGKSVKDTINDHDWN
                     ANTFLPTVGKRGAAIIAARGLSSAASAANAAIDHMRDWALGTNGKWVTMGIPSNGEYG
                     IPKDVMFGYPVTCEGGKYHIVKDLPIDAFSQECINKTLAELQGEQDGVKHLL"
     gene            3853..4863
                     /locus_tag="PK518_12760"
     CDS             3853..4863
                     /locus_tag="PK518_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRM49825.1"
                     /translation="MTAAVPHPREVLLGSQAAALTLPVCDHYSGVEARMRKSLQLQAE
                     MLQEFGHCVFDVTLDCEDGAPVGQEREHAQLVAQLASNAAAGARVAARVHTVDHPAFA
                     QDMDIIAGQAGQRLCHIMIPKVESVDDVLQAERALLAAGAGHLPLHALIESPAAVHRA
                     FEIAAHPRIQSLSFGLMDFVSAHGGAIPASGMTSAGQFQHPLVVRAKLEIAAACHANG
                     KVPSHCVVTEFSDTAAMQAAAGRAAREFGYTRMWSIHPAQIRPILAAFAPEDAEIARA
                     ARIIAAAIDADWAPISEEGVLHDRASYRYFWQLLERAHQTGRALPQQAQAWFANPDSA
                     PT"
     gene            4919..5533
                     /locus_tag="PK518_12765"
     CDS             4919..5533
                     /locus_tag="PK518_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49826.1"
                     /translation="MKILVLSALLVLAPAWSMAQQAAQPAGKTVAKSAAKSTAAKPAQ
                     TAKAKAKPQAAVVAKAAPTSSRTRLHSGAMQVAAGISAAEAALTPEELAIADEVHTGR
                     MACELGAFVQVERDAQSPGRFHVEGKGFKFHMAPVASATGAVRLEDQQAGAVWIQIAN
                     KSMLMNQKLGQRMADECMSPQQFTVAEGFKKNPPPSLLDSPATK"
     gene            5653..>7127
                     /locus_tag="PK518_12770"
     CDS             5653..>7127
                     /locus_tag="PK518_12770"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HRM49827.1"
                     /translation="MLQAYRAHAAERAALGIPPLPLDAKQVADLIELIKNPPAGEAEF
                     LLDLLTHRVPPGVDDAAKVKASFLAAVAHGDVQVALISKARATELLGTMVGGYNVHPL
                     IELLDDADVAGVAAEALKKTLLMFDYFNDVAAKAKAGNAKAKEVLQSWADAEWFTSRA
                     KVAEKITVTVFKVPGETNTDDLSPAPDATTRPDIPMHYLAMLKNQRPDAAFKPEQDGV
                     RGPMQFIEDLKKKGHVVAYVGDVVGTGSSRKSATNSVIWATGEDIPFVPNKRFGGVTL
                     GGKIAPIFFNTQEDSGSLPIEVDVSKMEMGDVIDIYPYAGKIEKAGEKIADFALKSDV
                     LLDEVQAGGRINLIIGRSLTAKAREALGLAASTAFRLPQAPAPSKAGFTLAQKMVGRA
                     CGLPEGQGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAA
                     YPKPVDVKTHRTLPAFISNRGGVSLRPGDGVIHSWLNRLLLP"
BASE COUNT         1316 a         2374 c         2260 g         1177 t
ORIGIN      
        1 aaggcgttgg tcgatgcggc aaagatgcgg cccgcgccac cggtggccag cagcacggcc
       61 ttggcgtgca gctcgtacag gtcgccggtt tccagttcca gggcggttac accgaccacg
      121 tcgccggtgc tgttgcggat cagatccagc gccatccatt cgacgaagaa gttggtcttg
      181 gactcgacgt tcttctggta cagcgtgtgc agcatggcgt ggccggtgcg gtcggccgcg
      241 gcgcaggcgc gctgcacggg tttttcgccg tagttggcgg tgtggccgcc aaacgggcgc
      301 tggtagatgg tgccgtcggc gttgcggtca aacggcatgc cgaaatgctc aagctcgatg
      361 acgacgttgg gcgcttcgcg gcacatgaac tcgacggcgt cctggtcgga cagccagtcg
      421 ccgcccttga tggtgtcgta gaagtggtag tgccagttgt cctcgctcat gttgccgagc
      481 gacgccgata cgccgccctg ggcggccacg gtgtgcgagc gcgtggggaa gaccttggtc
      541 aggcaggcga cggacagacc ggcgcgcgag agttccagcg aggcacgcat gcccgagccg
      601 ccggcgccga cgatgacgac atcgaacttg cgcacggcaa tatctttttt ggtgtaactc
      661 atggttgtgg tgtgtggggt gctaatgggt tctgaaagaa gggtggggct atgggtgcag
      721 cgtgctcaca ggcgccacag cacctgcacg gcccaaccac cacaactgac cagccagatg
      781 gcgctgagcg cctgcagcac caggcgcagg ccggcgggct tggcgtagtc gtaccagaca
      841 ctgcacacgc cgacccaggc gtgccagatc agcgccacga tgaccgagaa ggtcagcgcc
      901 ttcatccact gcggtgaaaa aatgccggcc caggtgtcgt agccgatggg gcctttggtg
      961 aacagcagtt gcgccagcag cagcagggtg aaggccagca gcaggatggc ggtgatgcgt
     1021 tgcaccacga aatcgcgcca gccatagtgg gcaccgacaa cggtgcgctt ggagccatag
     1081 gtcacagcca tggtgtcatt ccttttcttg ttgaatcagt acaggccaaa cagcttggcg
     1141 ccgaggatca gcgtcaggcc gatgctgaac accagcgtgg ccacggccga ctggcggcca
     1201 aagtccttgg tggtcttgtg cgacacatcc aggtacaggt ggcgcacacc ggcggccagg
     1261 tggtgcaggt aggcccagat cagggccagc gtcaccagct tgatgaacca gccgggcacg
     1321 aagcccaggc cgacgttgaa ggcggcgctg aaacggccaa acgaaatctc cgaggacacc
     1381 gaggtgtcga acatccagat caccagcggc agcaggatga acatgagcat gccgctgacc
     1441 cggtgcagga tggagatgaa accggccagc ggcaggcggt aggttctgac atcggaaaag
     1501 aggttgaggt tgcgaaactc gggcctcttc tttttggcta gctctgtcat ggtgggtact
     1561 ttcttggtgg taactgcgtg gtaatcgcgc ggtgcagaca acccaaaatt ctattgcaat
     1621 gcaatacgga ggtgatgcgg cgactgcgta tttgcgctaa ttgtgtcgaa tgtcaggcgt
     1681 gagtcagttc gattcggccg tcctgccgcg gtcagttcag cttgttgtgg tagtggtggg
     1741 tgtcggtgcg gtacaggccg cggcgcaact ccatgggcac atcgttgtag gtgtaggcca
     1801 gccgctccac gctcagcagc ggcgtgccgg cgggcacgtt gagccactgg gcctgcgttt
     1861 cgtcgggcag cacggcgcgg attttttcct cggcacgcac catgcgcacg ccaaagtcca
     1921 gctcgaacat ggcataggtg ggcccctggt agtgcgccag ctgctcggcc gtcagtccct
     1981 tgaatgcctg gccgggcagc caaatgtcct ccaggatggt cggtacgccg acaaaagaca
     2041 ggatacgttt gatctggatc acagaatcgc ctgcgcgcag cgccaggctg cgtgccatgt
     2101 cggcgctggc gcgcagccgg cggcattcca gaatggtgcg ctgggccggg ccttcctcgc
     2161 tggcgtcgcc ggcgtccggc agcagcttga ggaagcggta ttgcacctgg cgctcggcat
     2221 gcgtggccac aaacgtgccc ttgccctggc ggcgcgtgac caggttctcg gccgccagct
     2281 cgtcgatggc cttgcgcacc gtgccctggc tcacgcgaaa gcgtgcggcc agctcgatct
     2341 cgctcggaat ggcctcgccg ggcttccact cgccatgctg caggctgtgc agtatcagcc
     2401 ccttgatctg ctggtacagc gggctgaagg ccggcgtgac cgttgccgcg ccgccacttg
     2461 cggggggcgt gccttcggtg ctgggcggaa gcagggacga catggtggtg ctggctgtgc
     2521 ctgtgggcga gggtgtttcc agtcgctaat catatcttat ataagacata agacaaattg
     2581 accgtaaccc tgaatcgaca gtaaaatcag cagttgtttg cggagcacgg gctgccggtt
     2641 acaggccctg gtgcacgcac ccacaggttt tttctgtcac ttcctttttg gagttttctc
     2701 atgagcaaga agcccgttcg cgtcgcagtc accggcgccg caggtcaaat cggttacgcc
     2761 ctgctgttcc gcatcgcctc cggcgaaatg ctgggcaagg accagcccgt catcctgcaa
     2821 ctgctcgaaa ttcccgacga gaaggcccag aacgcgctca agggcgtgat catggaactg
     2881 gaagactgcg ccttcccgct gctggccggc atcgaggcgc actctgaccc catgacggcc
     2941 ttcaaggaca ccgactacgc gctgctggtc ggcgcccgtc cgcgcggccc cggcatggag
     3001 cgcgccgacc tgctggccgc caacgcccag atcttcaccg cccagggcaa ggccctgaac
     3061 gccgttgcca gccgcaatgt gcgcgtgctg gtggtgggca accccgccaa caccaacgcc
     3121 tacatcgcca tgaagagcgc gccggatctg ccgcgcgaga acttcaccgc catgctgcgc
     3181 ctggaccaca accgcgctgc cagccagatc gccaccaaga tgggcgtggc cgtgggcgac
     3241 atcgagaagc tggccgtgtg gggcaaccac tcgcccacca tgtacgccga ctaccgtttc
     3301 gccaccgtgg gcggcaagtc cgtcaaggac accatcaacg accacgactg gaacgccaac
     3361 accttcctgc ccaccgtggg caagcgcggc gcggccatca ttgccgcacg tggcctgtcg
     3421 tcggccgcct cggccgccaa cgccgccatc gaccacatgc gcgactgggc cctgggcacc
     3481 aacggcaagt gggtcaccat gggcatcccc agcaacggcg aatacggcat ccccaaggat
     3541 gtgatgttcg gctaccccgt gacctgcgaa ggcggcaagt accacatcgt caaggacctg
     3601 ccgatcgacg ccttcagcca ggaatgcatc aacaagaccc tggccgagct gcagggcgag
     3661 caggacggcg tcaagcacct gctgtaaaac gatagcgcgt tgcgcatgat agacaagggg
     3721 ttcagcgttg aaacatgctg aaatccttgt cctcacagcg caagcagctt ctatattgat
     3781 agaagtctgc tgtgttgcca tggcgctgcc gtggccacac accagatttt tgcccccaac
     3841 ccccgcatcc gcatgaccgc tgccgtcccc catccccgcg aagtactgct tggcagccag
     3901 gcggcggcct tgaccctgcc cgtgtgcgac cactacagcg gcgttgaggc acgcatgcgc
     3961 aagagcctgc agctgcaggc cgaaatgctg caggagtttg gccactgcgt gttcgacgtc
     4021 acgctggact gcgaggacgg cgctcccgtg ggccaggagc gcgagcacgc ccagctggtc
     4081 gcgcagctgg ccagcaacgc cgccgccggc gcccgcgtgg cggcgcgcgt gcacaccgtg
     4141 gatcaccccg catttgcgca ggacatggac atcattgccg gccaggccgg gcagcgcctg
     4201 tgccacatca tgattcccaa ggtggagtcg gtggatgacg tgctgcaggc cgagcgcgcg
     4261 ctgctcgccg ccggcgctgg ccacctgccg ctgcatgcgc tgatcgaatc gcccgccgcc
     4321 gtgcaccgcg ccttcgagat cgccgcccac ccgcgcatcc agagcctgag cttcggcctg
     4381 atggacttcg tctcggcgca tggcggcgcc attcccgcct cgggcatgac ctctgccggc
     4441 cagttccagc accccctggt ggttcgcgcc aagctggaga ttgctgccgc ctgccacgcc
     4501 aatggcaagg tgccctcgca ctgcgtggtc acagagttca gcgatacggc cgccatgcag
     4561 gccgccgccg gtcgcgccgc gcgcgaattc ggctacacac gcatgtggag catccacccg
     4621 gcgcagatcc gccccatcct ggccgccttc gcgcccgagg atgccgagat cgcccgcgcc
     4681 gcccgcatca ttgctgccgc catcgacgcc gattgggccc ccatcagcga agaaggcgtg
     4741 ctgcacgacc gcgccagcta ccgctatttc tggcagttgc tggagcgtgc acaccagacc
     4801 ggacgtgcat tgccgcaaca ggcgcaggcc tggtttgcaa atcctgacag cgcaccgacc
     4861 tgatacggat atttcgccgc cacaatcaag gttttcgcct atttccgccg gagtcatcat
     4921 gaaaatcctc gtcctttccg ccctcctggt gctggccccc gcctggtcca tggcgcagca
     4981 ggcggcgcag ccggccggca agaccgtcgc caagagcgcg gccaagagca cggccgccaa
     5041 gccagcgcaa acggccaagg ccaaggccaa gccgcaggcg gccgtcgtcg ccaaggccgc
     5101 gcccaccagc agccgcacgc gcctgcacag cggcgccatg caggtcgcgg ccggcatctc
     5161 cgccgccgag gccgcgctca cccccgaaga actggccatt gccgacgagg tgcacaccgg
     5221 ccgcatggcc tgcgaactgg gcgccttcgt gcaggtggag cgtgatgcgc agtcgcccgg
     5281 ccgcttccat gtcgaaggca agggcttcaa gttccacatg gcccccgtgg catcggccac
     5341 cggtgccgtg cgcctggagg atcagcaggc cggcgccgtg tggatccaga tcgccaacaa
     5401 gtccatgctc atgaaccaga agctcggcca gcgcatggcc gacgagtgca tgagcccaca
     5461 gcagttcacg gtggccgagg gcttcaagaa gaacccgccg cccagcctgc tggacagccc
     5521 cgcaaccaaa taagaaaacc cggcgcccgt cggcgggccg ctggcactaa ccaccttggc
     5581 ggtgggcatg cccgtggccg actggtgatc gcccccactt tgaaaaccct gaccattccg
     5641 gagaaagaag tcatgttgca agcctaccga gcccatgccg ccgaacgcgc cgccctcggc
     5701 atcccgccgc tgccgctgga tgccaagcag gtcgccgact tgatcgagct gatcaagaac
     5761 ccgcccgctg gcgaggccga gttcctgctg gacttgctca cccatcgcgt gcccccgggt
     5821 gtcgatgacg cggccaaggt caaggcatcc ttcctggccg ccgtggcgca tggcgacgtg
     5881 caggtggcgc tgatcagcaa ggctcgcgcc accgagctgc tgggcaccat ggtgggtggc
     5941 tacaacgtgc acccgctgat cgagctgctc gacgacgccg atgtggctgg ggtggctgcc
     6001 gaggcgctca agaagacgct gctcatgttc gactacttca acgacgtggc ggccaaggcc
     6061 aaggccggca acgccaaggc caaggaagtg ctgcaaagct gggctgacgc cgagtggttc
     6121 accagccgtg ccaaagtggc tgaaaaaatc accgtcaccg tcttcaaggt gccgggcgag
     6181 accaacaccg acgacctgtc gcccgcgccc gacgccacca cgcgccccga catccccatg
     6241 cactacctgg ccatgctgaa gaaccagcgc ccggacgcgg ccttcaagcc cgagcaggac
     6301 ggtgtgcgcg gccccatgca gttcatcgag gacctgaaga aaaagggcca cgtcgtcgcc
     6361 tacgtgggcg acgtggtcgg cacgggttcg agccgcaaat ccgccaccaa ctcggtcatc
     6421 tgggccaccg gcgaagacat tcccttcgtg cccaacaagc gctttggcgg cgtcacgctg
     6481 ggcggcaaga tcgctcctat cttcttcaac acgcaagagg attccggctc gctgcccatc
     6541 gaggtcgatg tgagcaagat ggaaatgggc gacgtcatcg acatctaccc ctacgcgggc
     6601 aagatcgaga aggccggcga gaagattgcc gatttcgctt tgaagagcga cgtgctactc
     6661 gacgaagtgc aggccggtgg ccgtatcaac ctgatcatcg gccgctcgct caccgccaag
     6721 gcgcgcgaag ccctgggcct ggccgcttcc accgccttcc gcctgccgca agctccggct
     6781 ccatccaagg ctggcttcac cctggcgcag aagatggtcg gccgtgcctg cggcctgccg
     6841 gaaggccagg gcattcgccc cggcacctac tgcgagccca agatgaccac cgtcggctcg
     6901 caagatacga ccggcccgat gacgcgcgac gaactgaagg acctggcctg cctgggcttc
     6961 agcgccgaca tggtgatgca aagcttctgc cacaccgccg cctaccccaa gccggtggac
     7021 gtgaaaaccc accgcacgct gcccgccttc atcagcaacc gtggcggcgt gtccctgcgc
     7081 ccgggcgacg gcgtgatcca cagctggctc aaccgcctgc tgctgcc
//
LOCUS       DAOMTQ010000152         7107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2435_length_7107_cov_21.187606, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000152 DAOMTQ010000000
VERSION     DAOMTQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2435_length_7107_cov_21.187606"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK518_12775"
     CDS             complement(<1..227)
                     /locus_tag="PK518_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM49828.1"
                     /translation="MPAVSFQSVSKIFPTSRGPFQALHSVSLDIQEGEFFGLLGPNGA
                     GKTTMISILAGLSRASQGRVAVLGHDVQADFA"
     gene            complement(287..556)
                     /locus_tag="PK518_12780"
     CDS             complement(287..556)
                     /locus_tag="PK518_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRM49829.1"
                     /translation="MLVLPTELTHKQASVTLRMLLQALKAERDSKVLVDAAALASFDS
                     SALAVLLECRRTALSEGRGFMVKALPPALASLAGLYGVRELLPAV"
     gene            complement(573..1229)
                     /locus_tag="PK518_12785"
     CDS             complement(573..1229)
                     /locus_tag="PK518_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM49830.1"
                     /translation="MMNRRTLTQSAAALAAAACLGWAPAVLAADEAPDALVKRLSADV
                     LETVKKDKSIKAGDVDKIMALVNKTILPYVNFRRMTAAAVGPAWRTATPEQQQRLQDE
                     FKQLLVRTYSGALSEVSDQTIVVKPMRAAAGDTDVLVRTEVRGRGEPVQLDYRLEKAP
                     GADGGWKIYNLNVLGVWLVETYRTQFAQEINAKGLDGLIAALAARNQANAGTGVAGTK
                     "
     gene            complement(1322..2053)
                     /locus_tag="PK518_12790"
     CDS             complement(1322..2053)
                     /locus_tag="PK518_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRM49831.1"
                     /translation="MTKIPRFKAWAARSAALALAGALLGGFASGPNANPADPLEPYNR
                     SMTRFNDNLDKAVLKPVATAYRDVTPQPVRTGVGNFFSNLGDAWSFANNLLQLRGREA
                     FDSMVRFSVNSVLGLGGVLDIASEMGVDRHKQDFGLTLGRWGVPTGPYLVLPVLGPST
                     VRDTAALPVDFYVGDLVDHVHDIPVRNSMYGLRAIDKRAQLLGAGTVLDEAALDPYSF
                     TRDVYLKLRAGASGSAEDGKLPEDY"
     gene            complement(2050..2532)
                     /gene="mlaD"
                     /locus_tag="PK518_12795"
     CDS             complement(2050..2532)
                     /gene="mlaD"
                     /locus_tag="PK518_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRM49832.1"
                     /translation="MQHSKNDLWVGLFVLLGAAALVFLALQSANLLQLNFRSGYTISA
                     RFDNIGGLKPKAAVRSAGVVVGRVESIGFDDKTFQASVELEMDRRYLFPKDSSLKILT
                     SGLLGDQYIGIEAGADEHNLANGDTVTATQSAVVLENLIGQFLYGKAEGSAAGAGGKK
                     "
     gene            complement(2566..3348)
                     /gene="mlaE"
                     /locus_tag="PK518_12800"
     CDS             complement(2566..3348)
                     /gene="mlaE"
                     /locus_tag="PK518_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HRM49833.1"
                     /translation="MSWWRPSDVGYAVRCKLADIGTGARLLARLVGLFGATMRRFGLV
                     RDQVHFLGNYSLSLIAVSGMFVGFVLALQGYNVLQLYGSANSLGLVVTLGLVRELGPV
                     LTALLFAGRAGTSLTAEIGLMRAGEQLSAMEMMAVDPVQRILAPRFWGGVIAMPLLAL
                     VFNAVGVIGGWLVGVVLIGVDAGAFWGQMQSGVDIFKDVGNGIVKSLVFGVAVTFIAV
                     LQGYVAKPTPEGVSRATTRTVVMASLAVLGLDFVLTALMFSI"
     gene            complement(3345..4193)
                     /locus_tag="PK518_12805"
     CDS             complement(3345..4193)
                     /locus_tag="PK518_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM49834.1"
                     /translation="MPPDASSSADALVQLRNVTFGYGERVVLRDLSLTVPRGKVTAIM
                     GASGGGKTTVLRLIGGQQRAQQGQVLLAGQDVGGMDTAQLYAARRRMGMLFQFGALFT
                     DMSVFENVAFPLREHTDLPEELIRDIVLMKLHAVGLRGARDLMPSQISGGMARRVALA
                     RAIALDPDLIMYDEPFAGLDPISLGTAAQLIRELNDAMGLTTIIVSHDLEETFRLADQ
                     VIILGPGTVAAQGTPEEVRASVDPLVHQFVHALPVGPVPFHYPGPAVAQDFGPIGVEQ
                     KSGGSQ"
     gene            4545..4985
                     /gene="speD"
                     /locus_tag="PK518_12810"
     CDS             4545..4985
                     /gene="speD"
                     /locus_tag="PK518_12810"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRM49835.1"
                     /translation="MSQQKKGLSAMKNYQTVVPIQASAMARRATGLHLIGDLYGCLCD
                     SRLMLDAEYLENFCKERVATAGLSTVGSLFHSFGEGGGVTGVVVLAESHLSIHTWPEA
                     GYVTLDVYVCNYTENNRPKAKRLFDDLLAAFNPNDPHLHRVDRA"
     gene            5068..>5239
                     /locus_tag="PK518_12815"
     CDS             5068..>5239
                     /locus_tag="PK518_12815"
                     /inference="COORDINATES: protein motif:HMM:NF036419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRM49836.1"
                     /translation="MTTTRLVAERLNADFGLYLSDTRTLAQRQSPWQHIEVFENPQFG
                     RVMRIDGAFMTSE"
     assembly_gap    5240..5339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5340..5942
                     /locus_tag="PK518_12820"
     CDS             <5340..5942
                     /locus_tag="PK518_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HRM49837.1"
                     /translation="AMAREWLASIHKGAFDDPRFALRLGDAREFIASTPERFDQIVLD
                     LTDPFGPAVELYTVEFYQACRRILNPGGVLSLHLGSPIHLQESMRRIGASVRAVFPVF
                     RPYLQYVPLYGTLWAMAMASDSTDPALLTPAEVDVRIAERGITDLQLYNGATHQALLA
                     QPNFVRALFAQAAEPLRSGDQLADVRDPAELPPVQVTIQL"
     gene            complement(6036..6815)
                     /locus_tag="PK518_12825"
     CDS             complement(6036..6815)
                     /locus_tag="PK518_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM49838.1"
                     /translation="MPNTTSDVPMIEARDVKKRFGHNEVLRGVSLSVARGEVVAIIGP
                     SGSGKSTFLRCLNHLETIDSGQVAIEGELLASTDAAGHCRYPQDAEVRRICAKTGMVF
                     QHFNLFPHLTVLQNLIAAPMVVQGVARDAAIARAEALLAKVGLAQKRDNYPARLSGGQ
                     KQRVAIARALCMQPDIMLFDEPTSALDPELTGEVLRTMRELAEERMTMLVVTHEMGFA
                     REVAQRVAFMDQGELITARPAAEFFADPGHERARAFLQHML"
     gene            complement(6796..>7107)
                     /locus_tag="PK518_12830"
     CDS             complement(6796..>7107)
                     /locus_tag="PK518_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM49839.1"
                     /translation="RGQYEAAKVLGMSYGQTMRRIVLPQMVRSILPPMSNETITLVKD
                     TSLIYVLALNDLLRAARGIVQRDFTTTPFIVAAAFYLVMTLVLTWGFQRLEQRYAKYD
                     Q"
BASE COUNT         1269 a         2358 c         2244 g         1136 t
ORIGIN      
        1 gcaaaatcgg cctgcacgtc gtgccccagc accgccacgc ggccctggct ggcgcgcgag
       61 aggcccgcca ggatgctgat catggtggtc ttgccggcgc cattggggcc cagcaggcca
      121 aagaattcac cctcctggat gtccaggctg acactgtgca gggcctgaaa cggcccccgg
      181 gaggtgggaa agatcttgga gacggattgg aaggaaactg cgggcatgag aaccggagat
      241 tttaagcgcc gccccccttc agggccttgc cggcgcaata aagccctcaa accgcgggca
      301 gcagctctcg cacaccgtac aggccggcca ggctcgccag cgccggcggc aaggccttga
      361 ccatgaagcc gcggccctcg gacagggccg tgcgacggca ctccaacagc acggccaggg
      421 ccgatgagtc aaagctggcg agcgccgcgg cgtcgaccag cacctttgaa tcgcgctcgg
      481 ccttgagcgc ctgcaacaac atgcgcaggg tgaccgaggc ctgcttgtgc gtgagttcag
      541 tgggcagcac cagcatggag gaagcgccct gtttacttcg ttccggcgac accggtgccg
      601 gcattggcct ggttgcgtgc ggccagggct gctatcaggc catccagccc cttggcattg
      661 atctcctgcg caaactgggt gcggtaggtc tccaccagcc acacgccgag cacgttcagg
      721 ttgtagattt tccagccacc gtctgcacca ggggcttttt ccaggcggta atcgagctgc
      781 accggctcac cacggccgcg aacctcggtg cgcaccagca cgtccgtgtc gcccgctgct
      841 gcgcgcatgg gcttgacgac gatggtctga tccgacacct cgctcaatgc gcccgaatag
      901 gtgcgcacca ggagctgctt gaattcatcc tgcagccgct gctgctgctc cggcgtggcc
      961 gtgcgccagg ccgggcccac ggcggcagcc gtcatgcggc ggaagttcac atagggcagg
     1021 atggttttgt tcaccagcgc catgatcttg tccacgtcgc cggccttgat cgacttgtcc
     1081 ttcttcaccg tctccagcac atcggcggac aggcgcttga ccagcgcatc gggtgcctcg
     1141 tcggcggcaa gcacggcggg tgcccagccc agacaggctg cagccgccag ggcggcagcc
     1201 gattgcgtga gggtgcggcg attcatcatg gaagaaatcc ttggagttgt tctggattgt
     1261 gcaaaggcag aggccaacgc agtgcgtcgc gcgcatgtgc gaatgcaaac aagtgcaagc
     1321 gtcagtaatc ctcgggcagc ttgccgtcct cggcgctgcc agaagcaccg gcgcgcagct
     1381 tcaggtacac gtcgcgcgtg aaactgtagg gatcgagcgc cgcctcatcg agcaccgtgc
     1441 ccgcgcccag cagctgcgcg cgcttgtcga tggcgcgcag gccatacatg ctgttgcgca
     1501 cgggtatgtc gtgcacatgg tctaccagat caccgacgta gaagtccacc ggcagcgccg
     1561 ctgtgtcccg caccgtggac gggcccagca cgggcagcac cagatagggc ccggtaggca
     1621 cgccccagcg gcccaaagtc aggccaaagt cctgcttatg gcgatcgaca cccatttcgc
     1681 tggcgatgtc gagcacgccg cccaggccga gcacgctgtt gacactgaag cgcaccatgc
     1741 tgtcaaaggc ctcgcgcccg cgcagctgca gcaggttgtt ggcgaaggac caggcgtcgc
     1801 ccaggttgga aaagaagttg cccactccgg tgcgcacggg ctgcggcgtc acatcgcgat
     1861 aggccgtggc cacgggcttg agcacggcct tgtccaggtt gtcgttgaac cgggtcatgc
     1921 tgcggttata gggctctaag ggatcggccg ggttggcgtt cggcccgctg gcaaaaccac
     1981 cgagcagcgc gccagccagc gccagtgccg cgctgcgcgc ggcccaagcc ttgaatcgtg
     2041 ggattttggt cattttttac ctcctgcacc tgcagcgctg ccttcagcct tgccgtagag
     2101 gaactgcccg atcaggtttt ccagcaccac ggcggactgc gttgccgtca cggtatcacc
     2161 gttggccagg ttgtgctcgt cggcaccggc ctctatgccg atgtattggt cacccagcag
     2221 gccgctggtc aaaatcttca aggagctatc cttgggaaac aggtagcgcc tgtccatctc
     2281 cagctccacg ctcgcctgaa aagtcttgtc atcgaagcct atcgactcca cgcgccccac
     2341 gaccacgccg gcgctgcgta ccgcggcctt gggcttcagg ccgccgatgt tgtcaaagcg
     2401 cgccgagatg gtgtagccgg agcggaagtt gagctgtagc aggttcgccg actgcaaggc
     2461 cagaaagacc agcgccgcgg cgccgagcag gacgaacagg ccaacccaga gatcattctt
     2521 ggagtgctgc atggtgaaat ttcccgatgg cggtgctgtc cgccctcaaa tactgaacat
     2581 caaggccgtg aggacaaaat cgagccccag cacggcaagc gaagccatga ccaccgtgcg
     2641 cgtggtggcg cgcgacacgc cctcgggcgt gggcttggcc acatagccct gcagcacggc
     2701 gatgaaggtg acggcgacgc cgaagaccag gctcttgacg atgccgttgc ccacatcctt
     2761 gaagatatcg acgccacttt gcatctggcc ccagaaggca ccggcatcga cgccgatcaa
     2821 caccacgccg accagccagc cgccgatcac gcccacggca ttgaacacca gcgccaagag
     2881 cggcatggcg atcacgccgc cccagaagcg tggcgccagg atgcgctgca ccggatccac
     2941 agccatcatc tccatggccg agagctgttc gcccgcccgc atcagcccga tctcggccgt
     3001 gagcgaggtg cccgcgcggc cggcgaacag cagcgccgtg agcacgggcc ccagctcgcg
     3061 caccaggccc agcgtgacca ccagacccag ggaattggcc gagccgtaga gctgcagcac
     3121 gttgtagccc tgcagcgcca gcacgaagcc cacgaacatg cccgacacgg cgatgaggga
     3181 cagcgaatag ttgcccagga agtgcacctg gtcgcgcacc aggccaaagc gccgcatggt
     3241 cgcgccgaac aggccgacga ggcgcgcgag caggcgcgca cccgtgccga tatcggcgag
     3301 cttgcagcgc acggcatagc ccacatccga cggccgccac cagctcattg actgcctcca
     3361 gacttctgct cgacgccgat ggggccaaag tcctgcgcca cggcggggcc ggggtagtgg
     3421 aacggcaccg ggcccacggg caaggcatgc acgaactggt gcaccagcgg atcaacgctg
     3481 gcgcgcacct cctcgggcgt gccctgggcg gccacggtgc cggggccgag gatgatgacc
     3541 tggtcggcca ggcggaaggt ctcctccagg tcatgcgaca cgatgatggt ggtcaggccc
     3601 atggcgtcat tgagctcgcg gatcagctgc gccgccgtgc cgagcgatat cggatccagc
     3661 ccggcgaagg gctcgtcgta catgatcaga tcggggtcga gcgcgatggc ccgcgccagc
     3721 gccacgcgcc gcgccatgcc gcccgagatc tggctcggca tgaggtcgcg cgcgccgcgc
     3781 aggcccacgg cgtgcagctt catgagcacg atgtcgcgga tcagctcctc gggcaggtcg
     3841 gtgtgctcgc gcagcgggaa ggccacgttc tcgaacacgc tcatgtcggt gaacagcgcg
     3901 ccgaactgga acagcatgcc catgcgccgg cgcgcggcat acagctgcgc ggtatccatg
     3961 ccgcccacgt cctggccggc cagcagcacc tggccctgct gcgcgcgctg ctggccgccg
     4021 atcaggcgca gtaccgtggt cttgccgccg cccgaagcgc ccatgatggc cgtgaccttg
     4081 ccgcgcggca ccgtcagcga caggtcgcgc agcaccacgc gctcgccata cccgaaggtg
     4141 acgttgcgca gctgcacgag ggcatcggct gaagaggatg cgtctggggg catggtgatg
     4201 gatggggggc ttgccttacc ggcagaaatc ttgcaaagct ctacatcata ggtgcaaacc
     4261 cgctggtgcc agccagcagt ttgtgcaatt aagcaacagc gcatataatt agcggttcta
     4321 tagaaatgtc tcgcccttgg ttttttcagg gcgacatggc tgtcggtggt gcaagggcga
     4381 cgtggcgcgc ccagcacaag cgaaggtgcg gcaacggccc gggcaagcaa attgcatggg
     4441 ccagcggcat tcccccctcc cgcttggcat tcagcaccca tgccggcagc gcaccgggtg
     4501 gcatgcgtag atgaggctca tttgccccgc atgccgtttt tgtcatgtcc caacaaaaga
     4561 agggcctgag tgcaatgaag aactaccaga ccgtcgtccc catacaagcc tccgccatgg
     4621 cacgccgcgc caccggcctg catctgatcg gcgatctgta tggctgcctg tgtgacagcc
     4681 ggctgatgct ggacgctgaa tatctcgaga atttttgcaa ggaacgcgtg gccaccgccg
     4741 gcctgtccac ggtcggctcg ctgtttcaca gcttcggcga gggcggcggc gtgaccggcg
     4801 tggtcgtgct ggccgaatcg cacctgtcca tacacacctg gcccgaggca ggctacgtca
     4861 cgctcgacgt ctacgtctgc aactacaccg aaaacaaccg ccccaaggcg aaaaggctgt
     4921 ttgacgacct gctggccgcg ttcaacccga acgacccgca cctgcaccgc gtcgatcgcg
     4981 cctgaccccc aaaaaatagc cgccctcccc tgctaccgca tggggagcgt cattcattgc
     5041 gtgcgtcgca cctgcaggag ccccaccatg accaccaccc gcctggttgc cgaacgcctg
     5101 aacgccgatt tcggcctgta tctgagcgac acgcgcacgc tggcacaaag gcagtcgccc
     5161 tggcagcaca tcgaggtgtt tgagaacccg cagttcggcc gcgtgatgcg catcgacggc
     5221 gccttcatga ccagcgagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     5341 ccatggcgcg cgaatggctc gccagcatcc acaagggcgc cttcgacgac ccgcgctttg
     5401 cgctgcgcct gggcgatgca cgcgagttca tcgcctccac gccagagcgt tttgaccaga
     5461 tcgtgctgga tctgacagac ccgttcggcc cggcggtgga gctgtacacg gtggagttct
     5521 accaggcctg tcggcgcatc ctgaaccccg gcggcgtgct gtcgctgcac ctgggctcgc
     5581 cgatccacct gcaggagagc atgcgccgca taggcgcgtc ggtgcgcgcg gtgttccccg
     5641 tgtttcgtcc ctatctgcaa tatgtgccgc tgtacggcac gctgtgggcc atggccatgg
     5701 cgtcggacag caccgacccg gcgctgctca cgcccgccga ggtggacgtg cgcattgccg
     5761 agcgcggcat caccgacctg cagctgtaca acggcgccac gcaccaggcg ctgctggccc
     5821 agccgaactt cgtgcgcgcc ctcttcgccc aggcggccga gccgctgcgc agcggcgatc
     5881 agctggccga tgtgcgcgac ccggcggagc tgccgccggt gcaggtgaca atacagctat
     5941 aaaaatagta gctgtctgcg caataccaac aagcgctaca gccaaaaaac acctgattca
     6001 ccgggagcgc cggcgtctcg ccggcgtcca acccatcaca acatgtgctg cagaaaggcg
     6061 cgcgcgcgct catggcccgg atcggcaaag aactccgccg ccgggcgcgc cgtgatcagc
     6121 tccccctggt ccatgaaggc cacgcgctgc gccacctcgc gggcaaaacc catctcgtgc
     6181 gtgaccacca gcatggtcat gcgctcctcg gccagctcgc gcatggtgcg cagcacctcg
     6241 ccggtcagct cggggtcgag ggcgctggtg ggctcgtcga acagcatgat gtcgggctgc
     6301 atgcacaggg cgcgggcaat ggccacgcgc tgcttttgtc cgccggacag gcgcgcgggg
     6361 tagttgtcgc gcttttgcgc caggcccacc ttggccagca gcgcctcggc acgcgcaatc
     6421 gcggcatcac gcgccacgcc ctgcaccacc atgggcgccg ctatcaggtt ctgcagtacg
     6481 gtcaggtgcg ggaacaggtt gaaatgctgg aacaccatgc cggtcttggc gcagatgcgg
     6541 cgcacctcgg cgtcctgcgg gtaacggcaa tggcccgcgg cgtcggtgct ggccagcagt
     6601 tcaccctcga tggcgacctg gccgctgtct atcgtctcca ggtggttcag gcagcgcaga
     6661 aaggtgctct tgcccgagcc cgacggcccg atgatggcca ccacctcgcc gcgcgccacc
     6721 gacagcgaca cgccacgcag cacctcgttg tggccgaagc gcttcttcac gtcgcgcgcc
     6781 tcgatcatgg gcacatcact ggtcgtattt ggcatagcgt tgttccaggc gctggaagcc
     6841 ccaggtcagc accagggtca tgacgaggta gaaggcggcg gcgacgatga acggcgtggt
     6901 cgtgaagtcc cgctgcacga tgccgcgcgc ggcgcgcagc aggtcgttga gcgccagcac
     6961 atagatgagg gaggtgtcct tgaccagggt gatggtctcg ttgctcatgg gcggcaggat
     7021 gctgcgcacc atctgcggca gcacgatgcg gcgcatggtc tggccgtagc tcatgcccag
     7081 caccttggcg gcctcgtact ggccgcg
//
LOCUS       DAOMTQ010000153         7058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2463_length_7058_cov_25.975296, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000153 DAOMTQ010000000
VERSION     DAOMTQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2463_length_7058_cov_25.975296"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2097)
                     /locus_tag="PK518_12835"
     CDS             complement(<1..2097)
                     /locus_tag="PK518_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRM49840.1"
                     /translation="MSTTVGIKVDDLLRERIRNAAQNLNRTPHWLIKQAVLQYVDALE
                     RGASTIRIFGTDDGVPDDGKDEVQPQAQHEVQPFLEFAQSILPQTPLRAAITAAWHRP
                     ETECLPALLPLARTQDEAQAAKVTELATRLVQGLRDAPVASGVAALVQEFSLSSQEGV
                     ALMCLAEALLRIPDKATRDVLIRDKISRGDWKSHVGRSPSLFVNAAAWGLVLTGKLTS
                     TSSEKSLSSALTRVIGKGGEPLIRQGVHRAMKLMGEQFVTGQNIAEALANSRTYEKQG
                     FRYSYDMLGEAAATEHDAQRYLRDYEQAIHAIGAASAGRGIFEGPGISIKLSALHPRY
                     SRAQYDRVMGELLPRVLHLAELAKKYDIGMNIDAEEADRLEISLDLMEALCAAPSLKG
                     WSGIGFVVQAYQKRCPHVIDYLIDLARRSRRRLMVRLVKGAYWDSEIKRAQLDGQAGY
                     PVYTRKVYTDVSYLACARKLLEAPDAIYPQFATHNAQTLSSIYQLAASVGGSYYSGQY
                     EFQCLHGMGEPLYAQVTGAASEGKLARPCRIYAPVGSHETLLAYLVRRLLENGANTSF
                     VNRIGDASVPVSELVVDPVVEVMQIASQEGRLGAPHPRIALPTALFAGLGAQARANSQ
                     GVNLANEQQLASLAAALLYSTRQTYLAAPAGVELPADPARTAGWQALPNPAELRDTVG
                     WVREASADEVQTAAARA"
     gene            complement(2227..3312)
                     /locus_tag="PK518_12840"
     CDS             complement(2227..3312)
                     /locus_tag="PK518_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRM49841.1"
                     /translation="MSNFEQFVGTRPVSEQHAFDTEALTAWLSQHVQGFAGPLRVEMF
                     KGGQSNPTYQLITPARSYVMRSKPGPVARLLPSAHAIEREYAVMRGLAGSDVPVPQML
                     ALCEDESIIGRAFYIMEFMAGRVLWDQSLPGMSVLERGAIYDEMNRVIAALHSVDYAA
                     RGLGGYGKPGNYFERQIGRWSKQYQASITEPIAEMDRLMQWLPEHMPASARDESRVSI
                     VHGDYRLDNLMFHPTEPRAIAVLDWELSTLGHPLADFAYHCMSWHIPATLSRGIGGLD
                     IAALGIPQEADYIRRYCQRTGIQDVDSLHADWNFYLAYNMFRIAAILQGIAKRVEAGT
                     AASTQAKAAGATARPMAELAWTFAQKR"
     gene            complement(3343..4062)
                     /locus_tag="PK518_12845"
     CDS             complement(3343..4062)
                     /locus_tag="PK518_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM49842.1"
                     /translation="MDEPILTIEEREAINSGRWFSTLSPSLRHDILRCATVKRYKDGA
                     LMAARGEPPEGWIGCARGAVRVSSTSLSGKQITLTYVEPGIWFGDVAMFDGDRRTHDA
                     YAHGDTTVLHVARADFKKILAQHTELYEAMLRLHARRIRQLFGLVEDLNTLPLRARLA
                     KQLLHLVRSYGVPSLENGNEIRIGLQLAQEELAQLLGASRQRVNQELKAMEREDAIRI
                     EAGGLVVRDREALMRIAESGP"
     gene            complement(4257..4421)
                     /locus_tag="PK518_12850"
     CDS             complement(4257..4421)
                     /locus_tag="PK518_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49843.1"
                     /translation="MSAQSGSRVYLFDSEASSKLVLAAIVERTKNLVRALVVRQGRLG
                     FEAAQSSRYQ"
     gene            4677..6605
                     /locus_tag="PK518_12855"
     CDS             4677..6605
                     /locus_tag="PK518_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49844.1"
                     /translation="MFEVRPDAGKDLASSCLSARLVSGASTVSDSRVQVTPVLGGTVP
                     MVRVQARITADEPVLTVTLAAGCEGRVTRSYTFLADPPSGTPAVAQRPLDVAGFGAAA
                     RGTDGWGLADADAAATRAAARQRVLAGLEGEGALASGSARPARPSSAKTADRPARPRA
                     LAPVPAAPARLIVEPLDLWLDTPLTLRLAREEPLLVSAPDEARRAEFAALWKALNTSP
                     ENLRESMALLDKLQGEAAAQVRQAEAEKAAAAELRQRLAQVEEQSFSATWVYGLGGLL
                     ALAVGCIGWLLRRARRQETQAWQHSVARSEALQHGAVAAEGPSGQDDWPLEPDAADTW
                     QPSSLPEVDEPDVPVDAPDTIPVGPSAAATAPAAFEPTIQPAMAPATPKAARAQQILP
                     PDAVFDIQQQAEFFTSIGEHDQAIDVLRAHIEEHGDAMPWAYLESLRLFHMLGRTESF
                     NRQRLQFQERFNVRVPGFGQFHHAGQALEDYPEALAEIEALWSSPEVMGVLDGLLFRR
                     GEGADDERFDLTAFDDLLLLLAIAQTTPAHLRGAPAPRARTTPRANKVSLPPERSLDS
                     IAGDLSLMPSGYDVLTPEPAPRPMLDVDLSQTCQPDIAFDDHPTTAVTRMQGVGFGME
                     DDKLELRFELDQMDKKRF"
     gene            complement(6613..>7058)
                     /locus_tag="PK518_12860"
     CDS             complement(6613..>7058)
                     /locus_tag="PK518_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM49845.1"
                     /translation="FINDHWREAMAAGAYGAHLGQEDLDALTREELDELRASGLRLGV
                     STHGYAEMLRADAASPSYIAMGAVFPTTLKKMATVPQGVARLAAYARLMRGYPQVAIG
                     GIGLEQFGQVRATAVGSIAVVRALVNAEQPEAAAGRLMHALRAGL"
BASE COUNT         1099 a         2280 c         2454 g         1225 t
ORIGIN      
        1 ggcgcgtgcg gcggcggtct gcacctcgtc ggcgctggcc tcgcgcaccc agcccacggt
       61 gtcgcgcagc tcggccgggt tgggcagggc ctgccagccc gcggtgcgcg ccgggtcggc
      121 cggcagctcc acaccagcgg gtgcggccag ataggtttgg cgcgtgctgt agagcagggc
      181 ggcggccagt gacgcgagct gctgctcgtt ggccaggttc acgccctgcg agttggcgcg
      241 ggcctgcgcg cccaggccgg cgaacagcgc tgtgggcagg gcaatgcgcg ggtgcggcgc
      301 gcccaggcgg ccttcctggc tggcgatttg catgacttcg accaccgggt cgacgaccag
      361 ctcggacacc ggcacgctgg cgtcgccaat gcggttgacg aacgaggtgt tggcgccgtt
      421 ctccagcaga cggcgcacca ggtaggcgag cagcgtctcg tggctgccca ccggggcgta
      481 gatgcggcag ggccgcgcca gcttgccttc cgacgccgcg ccggtgacct gcgcgtacag
      541 cggctcgccc atgccgtgca ggcactggaa ttcgtactgg ccgctgtagt agctgccgcc
      601 cacgctggcc gccagctggt agatggagga cagcgtctgc gcgttgtgcg tggcgaactg
      661 cgggtagatg gcgtcggggg cctccagcag cttgcgtgcg caggccaggt agctcacgtc
      721 ggtgtagacc ttgcgcgtgt agaccgggta gccggcctgg ccgtccagtt gggcgcgctt
      781 gatctcgctg tcccagtagg cacccttgac caggcgcacc atcaggcggc ggcggctgcg
      841 gcgggccagg tcgatcagat agtcgatgac gtgcgggcag cgcttctgat acgcctgcac
      901 gacaaagccg atgccgctcc agcccttcaa gctgggcgcg gcgcacaggg cttccatcag
      961 gtcgagcgag atctccaggc gatcggcctc ctcggcgtcg atgttcatgc cgatgtcgta
     1021 cttcttggcc agctcggcca ggtgcagcac gcgcggcagc agctcgccca tgacgcggtc
     1081 gtactgcgcg cgcgagtagc gcgggtgcag ggccgagagc ttgatcgaga tgcccgggcc
     1141 ttcaaaaatg ccgcgcccgg cggacgctgc gccgatggcg tggatggcct gctcgtagtc
     1201 gcgcaggtag cgctgggcgt catgctcagt ggccgcggcc tcgcccagca tgtcgtagct
     1261 gtagcgaaag ccttgctttt cataagtgcg gctattggcc agcgcctcgg caatgttctg
     1321 gccggtgacg aactgctcgc ccatcagctt catggcgcgg tgcacgccct ggcggatcag
     1381 cggctcgccg cccttgccga tgacgcgcgt cagcgccgac gacaggctct tctcgctgct
     1441 ggtactggtc agcttgccgg tcagcaccag gccccaggcg gcggcgttga cgaacagcga
     1501 gggcgagcgg ccgacgtgcg acttccagtc gccgcggctg atcttgtcgc ggatcagcac
     1561 gtcgcgcgtg gccttgtcgg gaatacgcaa gagcgcctcg gccaggcaca tcagcgccac
     1621 gccttcctgg ctggacagcg aaaactcctg caccagcgcc gccacgccgc tggccacggg
     1681 cgcgtcgcgc aggccctgca ccaggcgcgt ggccagctcg gtcaccttgg cggcctgggc
     1741 ctcgtcctgg gtgcgcgcca gcggcagcag cgccggcagg cactcggtct cggggcggtg
     1801 ccaggcggcg gtgatggcgg cgcgcagcgg cgtctgcggc aggatggact gggcaaattc
     1861 gaggaagggt tgcacctcgt gttgcgcctg gggttgcact tcgtccttgc catcgtcagg
     1921 cacgccgtcg tcggtgccaa agatgcggat ggtgctggcg ccacgttcca gcgcatcgac
     1981 atattgcagt accgcctgct tgatcagcca gtgcggggtg cggttcagat tctgggcggc
     2041 gttgcggatg cgctcgcgca gcaggtcatc gaccttgatg ccaacggtag tgctcatggg
     2101 gaagaaaaag gtgcaaccaa tgaagtctgc gaatatcgca aaggttgcac ctctggcgga
     2161 ctagggcaaa ccctaggtaa tggtttgcat ctgtatcacc gagcccattg gcggagcttc
     2221 actctcttat cgtttctgtg cgaacgtcca ggccagttcg gccatggggc gggccgtggc
     2281 tccggcggcc ttggcctggg tgctggcggc cgtgccggct tcgacgcgct tggcgatgcc
     2341 ctgcaggatg gcggcgatgc ggaacatgtt gtaggccagg tagaagttcc agtccgcgtg
     2401 cagcgagtcc acatcctgga tgccggtgcg ctggcaatag cgccggatgt agtccgcctc
     2461 ttgcggtatg cccagggcgg ctatgtccag cccgccgatg ccgcgtgaca gcgtggccgg
     2521 gatatgccag ctcatgcagt ggtaggcaaa gtcggccagc ggatggccca gggtggacag
     2581 ctcccagtcg agcaccgcga tggcacgtgg ctcggtgggg tggaacatga ggttgtccag
     2641 ccggtagtcg ccatgcacga tggagacgcg gctttcatcg cgggcgctgg cgggcatgtg
     2701 ctcgggcagc cattgcatga gcctgtccat ctcggcaatc ggctcggtga tggaggcctg
     2761 gtactgcttg ctccagcggc cgatctggcg ctcgaagtag ttgcccggct tgccgtagcc
     2821 gcccaggccg cgcgcggcgt agtcgacgct gtgcagcgcc gcgatgacgc ggttcatctc
     2881 gtcgtagatg gcgccgcgct cgagcacgct catgcccggc agggattggt cccagagcac
     2941 gcggccggcc atgaattcca tgatgtagaa ggcgcgcccg atgatggatt cgtcctcgca
     3001 cagcgccagc atctgcggca cgggcacgtc gctgccggcc aggccgcgca tgaccgcata
     3061 ttcacgctct atggcgtggg ccgagggcag cagcctggcc accggcccgg gcttggagcg
     3121 catgacgtag ctgcgcgcgg gcgtgatcag ctggtaggtg gggttggact ggccgccctt
     3181 gaacatctcc acccgcagcg ggccggcaaa gccctgcaca tgctgcgaca gccaggcagt
     3241 cagggcctcg gtgtcgaagg cgtgttgctc ggaaacgggg cgggtgccga cgaattgctc
     3301 aaaattgctc atggggtcag atacaggggt tggaatgggg gctcatgggc cagattcggc
     3361 gatgcgcatc agtgcctcgc ggtcgcgcac caccaggccg ccggcttcga tgcggatcgc
     3421 atcctcgcgc tccatggcct tgagctcctg gttcacgcgc tggcgcgatg cgcccagcag
     3481 ttgtgccagt tcttcctggg ccagctgcag gccgatgcgg atttcgttgc cgttctccag
     3541 gctgggcacg ccatagctgc gcaccaggtg caacagctgc ttggccaggc gcgcgcgcag
     3601 gggcagggtg ttcaggtctt ccaccagacc gaacagctgg cgtatgcggc gcgcgtgcag
     3661 gcgcagcatg gcctcgtaga gttcggtgtg ctgggccagg atcttcttga aatcggcgcg
     3721 tgccacatgc aggacggtgg tgtcgccatg ggcataggca tcgtgcgtgc gccggtcgcc
     3781 gtcgaacatc gccacgtcgc cgaaccagat gccaggctct acataggtca gcgtgatctg
     3841 cttgcccgac agcgaggtgg agctgacgcg cactgcgccg cgcgcgcagc caatccagcc
     3901 ctcgggcggt tcgccgcgcg cggccatgag cgcgccgtcc ttgtagcgtt tcaccgtggc
     3961 acatcgcaat atgtcgtgac gcagggaggg ggagagggtg gaaaaccagc gaccggagtt
     4021 gatcgcctcg cgttcctcga tggtaagaat cggctcgtcc atggtctgtc ttgtgcgtga
     4081 ctgggatgcg agccattgtc gcgtcgggca cgggcgcggc agtgccgcgc tgtcacatgg
     4141 gcgtcccggg gcccgggcca gcgcaccagt gggcaggcaa gaccatcaga aagcagcctg
     4201 cattttgtgt atcaaaaacc ctatcaacgc ttataaacaa agcgcaataa gctatgttat
     4261 tgatagcggc ttgactgtgc agcttcaaac cccagccggc cctggcgcac aaccagggcg
     4321 cgtacaaggt tcttcgtgcg ctcgacgatg gccgccagca ccagcttgga gctggcttcg
     4381 ctgtcaaaaa gatacacccg gctaccggat tgggccgaca tttacgcgat tgcgcgccgc
     4441 atggccccaa tgcccacgat acccatcgaa gcaaatgtca tatccatgat caaattgtct
     4501 tttgggcata agaaccatat gagtttgtgt cagaatcaat aaaacattta gaaaccattg
     4561 tgaatatgcg taaggtaatc gttggcgcct gcttatgcac cgtggcctct ggcgttgccg
     4621 cgttgtcgct ggggccaagc cgcggcgcag tggtgctcgg ttcgcccatt gatctgatgt
     4681 ttgaggtacg gcccgatgcg ggcaaggatc tcgcatcgtc ctgtctgtct gcgcgcctgg
     4741 tttcaggcgc cagcactgtt tcagattcgc gcgtgcaagt cacgccggtg ctgggcggta
     4801 ccgtgcccat ggtgcgtgtg caagcgcgca tcaccgcaga tgagcccgtc ttgacggtga
     4861 cacttgccgc cggttgcgaa gggcgcgtga cgcgcagcta tacgtttctg gccgatccgc
     4921 catcgggcac cccggccgtt gcccagcgcc cgctggatgt tgccggtttt ggcgccgcgg
     4981 cgcgcggcac cgatggctgg ggcctggcgg atgccgatgc ggcggcgacg cgtgccgccg
     5041 cgcgccagcg cgtcctggcc gggttggagg gcgagggggc gctggccagc ggctctgcgc
     5101 gcccggcgcg tccatccagc gccaagaccg ccgacaggcc cgccaggccg cgtgcgctgg
     5161 cgccagtgcc ggccgccccg gcacgcctga tcgtcgagcc gctggatctg tggctggata
     5221 cacccctgac cttgcgcctg gcgcgtgaag agccgttgct ggtctcggcc cccgacgagg
     5281 ccaggcgtgc agaatttgcc gccctgtgga aggcgctgaa tacctcgccc gagaacctgc
     5341 gggagtccat ggcgcttctg gacaagctgc agggcgaggc cgcagcccag gtcaggcagg
     5401 ccgaggccga gaaggccgcc gccgccgagc tgcgccaacg cttggcacag gtggaggaac
     5461 agagtttttc cgccacctgg gtctatgggc tgggtggcct gctggcactg gccgtgggtt
     5521 gcatcggctg gctgctgcgg cgtgccaggc gccaggaaac ccaggcctgg caacattcgg
     5581 tggcccgcag cgaggcgctg cagcatggcg ccgtggcggc cgaagggccg agcggccagg
     5641 acgactggcc actggagccc gatgccgccg acacctggca acccagctcc ctgcctgagg
     5701 tagatgagcc cgatgtgcct gtcgatgccc ccgacaccat accggtggga ccgtcggcgg
     5761 cggccactgc acctgcggcg ttcgagccca ctatccagcc cgccatggcg cccgccacgc
     5821 cgaaggcggc gcgcgcgcag cagattctcc cgcctgatgc ggtattcgac atccagcaac
     5881 aggccgagtt tttcacctcg attggcgagc acgaccaggc catcgacgtg ctgcgcgcgc
     5941 atatcgagga gcatggcgac gccatgccct gggcctatct ggaatcgctg cgcctgttcc
     6001 acatgctggg gcgcacggag agcttcaatc gccagcggct gcagttccag gagcgcttca
     6061 acgtgcgggt tccgggcttt ggccagttcc accatgcggg ccaggcattg gaggactacc
     6121 ccgaggccct ggcggaaatc gaggcactgt ggtcatcgcc cgaggtcatg ggcgtgctcg
     6181 acggcctgct gttccgccgc ggcgaggggg ctgacgacga gcgtttcgac ctgaccgcct
     6241 tcgatgacct gctgctgctg ctggccattg cgcagaccac gcccgcgcat ctgcgcggcg
     6301 cgccggcgcc gcgtgcgcgc accacgccca gggccaacaa ggtgagcctg ccgcccgagc
     6361 gttcgctgga ctcgatcgct ggagacctgt cgctcatgcc ttcgggctac gatgtgctga
     6421 cccccgagcc ggcgccacgc cccatgctgg acgtggactt gtcgcagacc tgccagccgg
     6481 atatcgcatt cgacgatcac cccactaccg ccgtcacccg aatgcagggc gtgggtttcg
     6541 gcatggagga cgacaagctc gaactgcgtt tcgagctgga tcagatggac aagaagcgct
     6601 tctgaccgcg cgttacaacc cggcccgcag agcgtgcatc agccggccgg cggcagcctc
     6661 gggctgttcg gcgttcacca gggcccgcac cacggcgatg gagcctacgg ccgtggcccg
     6721 cacctggcca aactgttcca gaccgatgcc gccaatggcc acctgcggat agccgcgcat
     6781 gaggcgcgca taggccgcca ggcgtgccac gccctggggc acggtggcca ttttcttcag
     6841 cgtggtcggg aacaccgcgc ccatggcgat gtagctgggg ctggcggcgt cggcgcgcag
     6901 catctcggcg tagccgtggg tgctgacgcc caggcgcagg ccgctggcgc gcagctcatc
     6961 caattcttcg cgcgtgagcg cgtccagatc ctcctggccc aggtgcgcgc cataggcgcc
     7021 ggcggccatc gcctcgcgcc aatggtcgtt gatgaaca
//
LOCUS       DAOMTQ010000154         7032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2473_length_7032_cov_23.965315, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000154 DAOMTQ010000000
VERSION     DAOMTQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2473_length_7032_cov_23.965315"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK518_12865"
     CDS             <1..213
                     /locus_tag="PK518_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49846.1"
                     /translation="LADRGLSVIGQSANPLQKIAPSTWRDFSNRAKSCRVRSRQLLQS
                     LMPQGDSGLDGAVGAALGRFAPMLFY"
     gene            complement(210..383)
                     /locus_tag="PK518_12870"
     CDS             complement(210..383)
                     /locus_tag="PK518_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49847.1"
                     /translation="MSRIQDMAAPIARHHLHCMDNEGEESGKQRARSVGYVRYAIMPQ
                     MTSAAHGVSAARY"
     gene            520..912
                     /locus_tag="PK518_12875"
     CDS             520..912
                     /locus_tag="PK518_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM49848.1"
                     /translation="MFSHVMLGASDLDQSKQFYDAVLGALGAGPGFTDKKNRTFWRHA
                     GNTFAITAPINGEPACHGNGSTIGFAAQSPEQAAAAHAAGVAAGGTTCEDPPGWRGDH
                     GAGGMYLAYLRDPDGNKLCVLHRPPKAA"
     gene            930..1691
                     /locus_tag="PK518_12880"
     CDS             930..1691
                     /locus_tag="PK518_12880"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HRM49849.1"
                     /translation="MQLSEQWVLVTGGARGLGQAITRALAREGAGVVINYHRSEAAAL
                     ALAEELGPRAVALQADVTDAAAVQRLFAAARERTGASISAVVNNALAQFSFDGDARPK
                     LGEVAWERFSQQIEGAVKGALNTMQAALPAMRAQGFGRIVNVGTNLFQNPVVPYHDYT
                     AAKAALLSLTRTAANDLGPDGITVNMVSGGLLRTTDASRATPEAVFDLIASLTPLRRV
                     TTPEEFADAVLFFLSPWARAVTGQNLVVDSGLVKD"
     gene            1694..2386
                     /locus_tag="PK518_12885"
     CDS             1694..2386
                     /locus_tag="PK518_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRM49850.1"
                     /translation="MALTHPITAKWPAQHPDRLQLYSLPTPNGVKVSIALEEMQLPYE
                     AHKVSFESGDQLSPEFLSLNPNNKIPAIIDPDGPGGRPLALFESGAILIYLAEKTGQF
                     LPREPAARYETLQWLMWQMGGVGPMFGQLGFFHKFAGKDWEDKRPRERYAAESRRLLG
                     VLEQRLAASEWIMGGDYTIADIAVFPWVRNLVGFYNAGLLVGWADFPQVQRALDAFVA
                     RPAVQRGLQIPA"
     gene            2420..3754
                     /locus_tag="PK518_12890"
     CDS             2420..3754
                     /locus_tag="PK518_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRM49851.1"
                     /translation="MTASAPQKRGIPPAYLILVAMLVGIGVGYFVFVNFPDKQAAKEV
                     AGYISIMSDVFLRLIKMLIGPLVFSTLVVGIAHMGDAASVGRVFMKAMLWFVTASLVS
                     LVLGLVLANWLQPGHNLGLPLPDVGAATNLATAKFTLKEFVNHLVPKSFAEAMANNEI
                     LQIVVFSMFFGVALAALGEKGKTLVLVVEELAHVMLKITGYVMKLAPLAVLSAMAATV
                     AVNGLGILLKFAVFMGDFYLGLFILWALLIGAGFVFLGPRIFRLMVLIKEAFMLSFAT
                     ASSEAAYPKILDALDRFGVKRKISSFVMPMGYSFNLDGSMMYCTFATLFIAQAYDIHL
                     SLATQITMLMILMLTSKGMAGVPRASLVVIAATLNQFDIPEAGLLLILGVDTFLDMGR
                     SATNAVGNSIASAVVAKWEGQLLTTDEAAQHEQRMDEEAAARAHPIPGPDLA"
     gene            3751..4680
                     /locus_tag="PK518_12895"
     CDS             3751..4680
                     /locus_tag="PK518_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM49852.1"
                     /translation="MTALRHLLCAAPLLAALAVQAQDADSTPSNPGPQALTGTLAQVH
                     ASGSIALAYRGSSVPFSYLGAQGQPIGYSIELCKALVRAVEVAVQRPLEIRWVSVTSA
                     TRMQAIVSGQADLECGSTTSNLERQKTVAFSPTIFVAGTRLLVRKGEGIRDWRDLAHK
                     KVAVTAGTTNEQVMRNLSQRLKVPMTLLVAPDHAQAFAEVDSGAADAFATDDVLLYGL
                     VAQTPGAAKRLAVVGELLSYEPYAVMFRKADPQLAKLVTDSMRALARDGEIDRQYRRW
                     FLQKLPGSQQRLGLPMSAQLEAIIQSLAAAQVE"
     gene            4688..5407
                     /locus_tag="PK518_12900"
     CDS             4688..5407
                     /locus_tag="PK518_12900"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HRM49853.1"
                     /translation="MSHAVSELRAARLARSAKPFLARGGSRRPHCPACRLVTSHCQCA
                     LRPQVHTHAGICLLMHDTEPLKPSNTGWLIADLVADTHAFGWARTEVDPALLALLADP
                     QWQPYVVFPGEYAPPERVLQTLPEAAGAGPRPLFVLLDATWPEARKMFRKSPYLERLP
                     VLSLEGDVSRYRLRRSRRGEHLCTAEVAALCLDKAGDAQAGALLQAWLDVYSERYLLA
                     KNQRPVDPDSAAHRQLAAALA"
     gene            5458..6015
                     /locus_tag="PK518_12905"
     CDS             5458..6015
                     /locus_tag="PK518_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HRM49854.1"
                     /translation="MHRRQILAQLGAASLALLTGCARAAPGYTISQRQLQQALAERFP
                     RSYPLAGLLDLQLQAPRLTLLPERNQLNAVMDLAATGPLLARRYTGVFDVDFGLRYEA
                     QDRSIRAHRLQVNALQLEGLKPQAAALLQRYAQQLADQSLHGVVLHQLQDKDLAPIDA
                     LGLQPESINVTPRGLLVRFGPKPLS"
     gene            6238..>7032
                     /locus_tag="PK518_12910"
     CDS             6238..>7032
                     /locus_tag="PK518_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM49855.1"
                     /translation="MTQTPPAAPSRLSAAPPEPVVVITGASSGIGHATALAFARRGAR
                     VVLAARNADTLAPVAMACRAAGGQALGMPTDVTDPAAVLALADRAVRHFGRIDIWVNA
                     VGVGAVGRFDGVPLAAHRRVIEANLLGHVHGAHAALARFRAQGRGLLVNVISLGGWLP
                     TPYAAAYAASKFGLRGLSESLRAEVADLPLVHVCAVAPTVVDTPGLSHGANYTGRRLA
                     APLPMVDPHAVAQAIVELADNPEPRAVTWLGLGARPARLAHALAPQG"
BASE COUNT         1054 a         2490 c         2315 g         1173 t
ORIGIN      
        1 ctggccgata gggggctcag cgtcattggg cagtcagcaa acccgttgca gaaaattgcg
       61 ccatctactt ggcgcgattt ctcgaatcgc gccaaatcct gtcgcgtcag atcccgtcaa
      121 ctgctgcaga gcctgatgcc acaaggcgac tcgggtcttg acggggctgt tggcgctgca
      181 ctcgggcgct tcgcgccaat gctcttttat taatagcgtg cagcgcttac tccatgggct
      241 gcagaggtca tttgaggcat gattgcatag cgcacatagc ccactgatcg ggcccgttgc
      301 ttgccgcttt cttccccttc gttatccatg cagtgaaggt gatggcgcgc aattggcgcc
      361 gccatgtctt gaatgcggct catgtcttac ctgcctccaa aaaaagcaca ccgcaggcgc
      421 aggctgtcca ccgcgtgacc cgcagccgcc gccagggcca ctacgatacg gagcccacca
      481 tgcccatggt ggattcaccc cacaggagac cgatcaacca tgttcagcca cgtcatgctg
      541 ggagccagcg acctcgacca atccaagcag ttctacgacg ccgtcctcgg cgccctgggc
      601 gccggccccg gcttcaccga caagaagaac cgcactttct ggcgccacgc gggcaacacc
      661 tttgccatta ccgcgcccat caacggcgag ccggcctgcc acggcaacgg cagcaccatc
      721 ggcttcgcgg cccagtcgcc cgagcaggcc gctgccgccc atgccgccgg cgtggccgcg
      781 ggcggcacga cctgcgaaga ccccccgggc tggcgcggcg accatggcgc cgggggcatg
      841 tacttggcct atctgcgcga tcccgacggc aacaagctct gcgtgctgca ccgcccgccc
      901 aaggccgcct gaccccggag cgttcacgca tgcaactatc cgaacaatgg gtgctggtca
      961 ccggcggcgc ccgcgggctg ggccaggcga tcacccgcgc gctggcgcgc gagggcgcag
     1021 gcgtcgtcat caactaccac cgcagtgagg ccgccgcgct ggcgctggcc gaggagctgg
     1081 gcccgcgcgc cgtggcgctg caggccgacg tgaccgatgc cgccgccgtg cagcggctct
     1141 tcgccgcggc gcgcgagcgc acaggcgcca gcatcagcgc cgtggtgaac aacgcgctgg
     1201 cgcagttctc gttcgacggc gatgcgcgcc ccaagctggg cgaggtcgcc tgggagcgct
     1261 tcagccagca gatcgagggc gcggtcaagg gtgcgctcaa caccatgcag gcggcgctgc
     1321 ccgccatgcg cgcgcagggc ttcgggcgca tcgtgaacgt gggcacgaac ctgttccaga
     1381 acccggtggt gccctaccac gactacacgg cggccaaggc ggcgctgctg tcgctcacgc
     1441 gcacggcggc gaacgacctg gggcctgacg gcatcacggt gaacatggtc tcgggcggac
     1501 tgctgcgcac caccgacgcc agccgcgcca cgcccgaggc ggtgttcgac ctgatcgcca
     1561 gcctgacccc gctgcgccgc gtgaccacgc cggaggaatt cgccgacgcg gtgctgttct
     1621 tcctctcgcc ctgggcgcgc gcggtgaccg ggcagaatct ggtggtcgac agcggcctcg
     1681 taaaggattg accatggctc tcacccaccc gatcaccgcc aagtggcccg cccagcaccc
     1741 cgaccggctg cagctgtatt cgctgcccac gcccaacggc gtgaaggtgt cgatcgcgct
     1801 ggaggagatg cagctgccct acgaggcgca caaggtgagc ttcgagagcg gcgaccagct
     1861 ctcgcccgag ttcctgtcgc tgaacccgaa caacaagatc ccggccatca tcgaccccga
     1921 tggcccgggt ggtcggccgc tggcgctgtt cgagtccggc gccatcctca tctacctggc
     1981 cgagaagacg ggccagttcc tgccgcgcga gccggccgcg cgctacgaga cgctgcagtg
     2041 gctgatgtgg cagatgggcg gcgtggggcc aatgtttggc cagctggggt tcttccacaa
     2101 gtttgccggc aaggactggg aggacaagcg cccgcgcgag cgctacgcgg ccgagtcgcg
     2161 ccgcctgctg ggcgtgctgg agcagcgcct ggcggccagc gaatggatca tgggcggcga
     2221 ctacaccatt gccgacatag cggtctttcc gtgggtgcgc aacctggtgg gtttttacaa
     2281 tgccggcctt ttggtcggct gggccgactt tccccaggtg cagcgggcgc tcgatgcctt
     2341 cgtcgcgcgc cctgccgtgc agcgcgggtt gcagattccc gcctaagaac aacatccccg
     2401 catcccggaa ggactccaca tgaccgccag cgcaccgcaa aaaagaggca tcccgcctgc
     2461 ctatttgatt cttgtcgcca tgttggtagg cataggcgtg ggctactttg tcttcgtcaa
     2521 tttccccgac aagcaggccg ccaaggaggt ggcgggctac atctccatca tgtccgacgt
     2581 gttcctgcgc ctgatcaaga tgctgatcgg cccgctggtg ttctccacgc tggtggtggg
     2641 cattgcgcat atgggcgacg cggcctcggt ggggcgggtg ttcatgaagg ccatgctgtg
     2701 gttcgtcacg gcgtcgctgg tctcgctggt gctgggcctg gtgctggcga actggctgca
     2761 gcccggtcac aacctgggcc tgccgctgcc cgacgtgggc gcggccacca acctggccac
     2821 ggccaagttc acgctcaagg agttcgtcaa ccacctggtt cccaagtcct tcgccgaggc
     2881 catggcgaac aacgagatcc tgcagatcgt ggtgttctcg atgttcttcg gcgtggccct
     2941 ggcggccctg ggcgagaagg gcaaaaccct ggtgctggtg gtcgaggagc tggcccatgt
     3001 gatgctgaag atcaccggct atgtgatgaa gctcgcgccg ctggccgtgc tctcggccat
     3061 ggcggccacg gtggcggtga atggcctggg catcctgctc aagttcgccg tgttcatggg
     3121 tgatttctat ctgggcctgt tcatcttgtg ggcgctgctc ataggcgccg gcttcgtctt
     3181 cctggggccg cgcatcttca ggctcatggt gctgatcaag gaggccttca tgctgtcctt
     3241 tgccacggcc agctccgagg ccgcctaccc caagatcctg gacgcgctgg accgctttgg
     3301 cgtcaagcgc aagatctcca gcttcgtcat gcccatgggc tactcgttca atctggacgg
     3361 ctccatgatg tactgcacgt ttgccacgct gttcatcgcc caggcgtacg acatccacct
     3421 gtcgctggcg acgcagatca ccatgctgat gatcctgatg ctcacctcca agggtatggc
     3481 cggcgtgccg cgcgcctcgc tggtggtgat tgccgccacg ctgaaccagt tcgacattcc
     3541 cgaggccggc ctgctgctca tcctgggcgt ggacaccttt ctggacatgg gccgctcggc
     3601 caccaacgcg gtcggcaact cgattgccag cgccgtcgtc gccaagtggg aggggcagct
     3661 gttgaccacc gacgaagccg cccagcacga gcagcgcatg gacgaggagg cggccgcgcg
     3721 cgcccatccc attcccgggc cggatctggc atgaccgcgc tgcgccatct tctctgcgct
     3781 gcgcccctgc tggcggccct ggccgtgcag gcccaggacg cggacagcac acccagcaac
     3841 cccgggccgc aggcgctcac cggcaccctg gcccaggtgc atgccagcgg cagcattgcg
     3901 ctggcctacc ggggcagctc ggtgccgttt tcctacctgg gcgcgcaggg tcagcccatc
     3961 ggctactcga tcgagctgtg caaggcgctg gtgcgcgccg tcgaggtggc ggtgcagcgg
     4021 cccctggaga tccgctgggt gagcgtgacc tcggccacgc gcatgcaggc cattgtttcg
     4081 ggccaggccg atctggaatg cggctccacc accagcaacc tggagcggca gaagaccgtc
     4141 gccttctcgc ccaccatctt tgtcgccggc acgcggctgc tggtgcgcaa gggcgagggc
     4201 atacgcgact ggcgcgacct ggcgcataaa aaggtggcgg tgacggccgg caccaccaac
     4261 gaacaggtga tgcgcaatct gtcgcagcgc ctgaaggtgc ccatgacgct gctggtcgcg
     4321 cccgatcacg cccaggcctt tgccgaggtg gacagtggcg ccgccgacgc ctttgccacc
     4381 gacgacgtgc tgctctacgg cctggtggcg caaacccctg gtgcggccaa gcgcctggcc
     4441 gtggtgggcg agctgctgtc ctacgagccc tatgccgtca tgttccgcaa ggccgacccg
     4501 caactggcca agctggtcac cgacagcatg cgcgcgctgg cacgcgacgg cgagatcgac
     4561 cgccagtacc gccgctggtt cctgcaaaag ctgcccggca gccagcagcg cctgggcctg
     4621 ccgatgagcg cgcagctcga ggccatcatc cagagcctgg ccgccgccca ggtcgaataa
     4681 gcccttcatg agccacgccg tctccgagct gcgcgccgcc cgcctggcgc gcagcgccaa
     4741 gccctttctg gcacgcggtg gctcgcgccg cccgcattgc ccggcctgcc gcctggtcac
     4801 cagccactgc caatgcgcac tgcgcccgca ggtgcacacg catgccggca tctgcttgct
     4861 gatgcacgac accgagccgc tcaagcccag caataccggc tggctgatcg ccgatctggt
     4921 ggccgacact catgcttttg gctgggcgcg caccgaggtc gatccggccc tgctggcgct
     4981 gctggccgac ccgcaatggc agccctatgt ggtgttccct ggcgagtacg cgccgcccga
     5041 gcgcgtgctg cagaccctgc ccgaggccgc cggggcaggc ccgcgtccgc tgttcgtgct
     5101 gctggacgcc acctggccgg aggcgcgcaa gatgttccgc aagagcccct atctggagcg
     5161 cctgccggtg ctcagcctgg agggcgatgt ctcgcgctac cggctgcgcc gctcgcggcg
     5221 cggcgaacat ttgtgtaccg ccgaggtggc cgccctgtgc ctggacaagg ccggcgatgc
     5281 gcaggccggt gcgctgctgc aggcctggct tgatgtctac agcgagcgct acctgctggc
     5341 caagaaccag cgcccggtcg atccggacag tgcggcccac cggcaactgg ccgccgccct
     5401 ggcttgacgc aactgcttgc ctggcgcgcc atgcgcctgg ccacaatacg caccaccatg
     5461 caccgcagac agatccttgc ccagctgggc gccgcctccc tggccctgct gaccggctgc
     5521 gcccgcgcgg cgcccggcta caccatcagc cagcggcagc tgcaacaggc cctggccgag
     5581 cgctttccgc gcagctaccc gctggccggc ctgctggatc tgcagctgca ggcgccgcgc
     5641 ctgaccctgc tgcccgagcg caaccagctc aacgccgtga tggacctggc cgccaccggc
     5701 ccgctgctgg cgcgccgcta tacgggcgtt ttcgacgtgg acttcggtct gcgctacgag
     5761 gctcaggatc gcagcatccg cgcgcaccgg ctgcaggtga acgcgctgca gctggagggc
     5821 ctgaaacccc aggccgccgc gctgctgcag cgctatgccc agcagctggc agaccagtcg
     5881 ctgcatggcg tggtgctgca ccagctgcag gacaaggatt tggcgcccat tgacgcgctg
     5941 ggcctgcagc ccgagagcat caacgtcacg ccgcgtggtc tgctggtgcg ctttgggccc
     6001 aagccattgt cttgatcgct tctgttttaa tagctgtttg cgcttgatgg ataagcgtca
     6061 gagccggttt tactccctct ccctccggga gagggtgggg gtgagggctc acggcccagt
     6121 ctgaggcatg tgcctgaaca gggttgtttg ctttgacagg cacgggccat gtcctacgcc
     6181 ggtgcgtccg gcctgcggct acaaagaagg ggtttgcaac cgtccatgcc gcacaccatg
     6241 acccagaccc cgcccgccgc accgtcccgc ctgagcgcgg ccccgcccga gccggtggtg
     6301 gtgatcaccg gcgcctccag cggcataggc catgccacgg cgctggcgtt tgcgcgccgc
     6361 ggtgcacgcg tggtgctggc ggcgcgcaat gccgacaccc tggcccccgt ggccatggcc
     6421 tgccgcgccg ccggcggtca ggccctgggc atgcccaccg acgtgaccga ccccgccgcc
     6481 gtgctggccc tggccgacag ggccgtgcgc catttcgggc gcatcgacat ctgggtcaac
     6541 gccgtcggcg ttggggccgt ggggcgcttt gacggcgtgc cgctggccgc acacaggcgc
     6601 gtcatcgaag ccaacctgct cggccatgtg catggcgcgc atgcggcgct ggcgcgcttt
     6661 cgcgcccagg ggcgcggcct gctggtcaac gtcatctcgc tcggcggctg gctgcccacg
     6721 ccctatgccg cggcctatgc ggccagcaag ttcggcctgc gcggcctgtc cgagagcctg
     6781 cgcgccgagg ttgccgacct gccgctggtg catgtctgcg ccgtggcgcc cacggtggtg
     6841 gacacgcccg gcctgtcgca tggcgccaac tacaccggcc ggcgcctggc cgcgcccctg
     6901 cccatggtcg atccgcacgc ggtggcccag gccatcgtgg agctggccga caaccccgag
     6961 ccgcgcgccg tcacctggct gggcctgggc gcgcggccgg cgcgcctggc gcatgcgctg
     7021 gcaccccagg gc
//
LOCUS       DAOMTQ010000155         6765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2597_length_6765_cov_28.577645, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000155 DAOMTQ010000000
VERSION     DAOMTQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_6765_cov_28.577645"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..530
                     /locus_tag="PK518_12915"
     CDS             123..530
                     /locus_tag="PK518_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM49856.1"
                     /translation="MPTYSISDLAREFDLTTRAMRFYEDMGLLRPERAGPGGRNRVYS
                     GRDRARLKLTLRAKRLGLSLNEAKEIIDLYDSPRDTGVQLRKFLEVLAMHRKQLELQM
                     RDLQANLDELREHEREAMDLLGKIEPPTAETTA"
     gene            620..1810
                     /locus_tag="PK518_12920"
     CDS             620..1810
                     /locus_tag="PK518_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRM49857.1"
                     /translation="MSIATQLPGLNFQLGEDIDALRDAVREFAQSEIGPRAAEIDRDD
                     QFPMDLWRKFGDLGVLGITVPEEFGGANMGYLAHMVAMEEISRASASVGLSYGAHSNL
                     CVNQINRNGNPAQKAKYLPKLISGEHVGALAMSEPGAGSDVISMKLKAEDKGGYYLLN
                     GSKMWITNGPDADTLVVYAKTEPELGARGVTAFLIEKGMKGFSIAQKLDKLGMRGSHT
                     GELVFQDVEVPAENVLGMVNGGAKVLMSGLDYERAVLTGGPLGIMQSVMDNVVPYIHD
                     RKQFGQSIGEFQLIQGKVADMYTVLQAGRSFAYTVAKNLDMLGAEHVRQVRKDCASVI
                     LWTAEKATWMAGEGVQIFGGNGYINEYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELF
                     AETC"
     gene            1842..2504
                     /gene="can"
                     /locus_tag="PK518_12925"
     CDS             1842..2504
                     /gene="can"
                     /locus_tag="PK518_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HRM49858.1"
                     /translation="MPSSSINDLFEHNRAWAARMEREKPGFFTGLMAQQKPRYMWIGC
                     SDSRVPANQITGLEPGEVFVHRNVANVVVPTDLNCLSTIQYAVDQLKVEHLMVVGHYG
                     CGGVLAALEDVRVGLADNWIRHVKDVRDKHAALLDGIDLQWRHDALCELNAIEQVLNI
                     AHSTVMQDAWARGQKVQIHGWCYGLRNGLITNLHMTVPGVGGLHEVHQAAVEQVAQRA
                     RQ"
     gene            2538..4364
                     /gene="aceK"
                     /locus_tag="PK518_12930"
     CDS             2538..4364
                     /gene="aceK"
                     /locus_tag="PK518_12930"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRM49859.1"
                     /translation="MFPQRLDAPQAFDIAKAMLDGFNRHYRLFRAESARAKHRFETAD
                     WHGQQRAQRERIEFYDLRVKECVRRLDKEFNAGALPMVVWHQVKLHYIGLMVNHLRPE
                     LAETFFNSVTTKILHRTHFHNDFIFVWPAVSTEYIESEDPGARPTYRAYYPAGDGLHQ
                     AVADIIEQFALQRPFADLRRDVDCVVRAMQQQLGAAKLRSNFQIQVLTSLFFRNKGAY
                     VVGKLINGFTELPFALPILHGEQGTLVIDAALFGENDLQMLFSFARAYFMVDMEIPSA
                     YVQFLATLMPRKRRAELYISLGLAKQGKTLFYRDFLHHLRYSSDQFRIAPGIKGMVML
                     VFDLPSFPYVFKLIKDHFPAPKDTTREQVKAKYLLVKQHDRVGRMADTLEYSLVAFPR
                     DRFSDELIAEIQKHAPSQLEISDRDGDGRQEVIISHLYIERRMIPLNIHLQECLDAGL
                     DEPESASALEHAVLEYGNAIKDLVAANIFPGDMLWKNFGMTRNGKVVFYDYDEIEYLT
                     DCNFRRVPAPRCEEDELSGEVWYAVGPHDVFPETFGPFLLGNDAVRAAFMRHHADLLD
                     VDFWQSHKERIQAGHVYDVFPYDSTRRFGGVPLPTQPPGDIT"
     gene            4361..5551
                     /locus_tag="PK518_12935"
     CDS             4361..5551
                     /locus_tag="PK518_12935"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRM49860.1"
                     /translation="MSQHQDPVVIVSAARTPMGGFMSDFADLAAHDLGGVAIKAAVER
                     AGIAPALVEEVLFGNCLMAGQGQAPARQALFKGGLPQSTGAVTLSKMCGAGMEATILA
                     HDQLIAGSREVMVSGGMESMSGAPYLLKKGRGGYRMGHDKVFDHMMLDGLEDAYEPGR
                     SMGTFGEDCAAKYQFTREQQDAFAIASVTRAQEAARSGAFDAEIAPVTLKTRKGEVTV
                     SVDEGPAKARLDKIASLKPAFRKDGTITAASSSSINDGAAAMVLMRASTAQRLGCKPL
                     ARIVGHATHAQAPEWFSTAPVGATEKLLKKIGWQVGDVDLWEINEAFAVVPMALMADL
                     KIPHDKVNVNGGACALGHPIGASGARILVTLVHALKARGLQRGLATLCIGGGEATAMA
                     IELI"
     gene            5644..>6765
                     /locus_tag="PK518_12940"
     CDS             5644..>6765
                     /locus_tag="PK518_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM49861.1"
                     /translation="MLLTQDQEMIRDAVRAFAQEELWPHAARWDKEHHFPRDAHQGLA
                     ALGAYGICVPEDLGGAGLDYLSLALVLEEIAAGDGGTSTAISVTNCPVNAILMRYGNA
                     RQQRDWLAPLARGEMLGAFCLTEPHVGSDASALRTTAVRQGDEYVINGVKQFITSGKN
                     GQVAIVIAVTDKGAGKKGMSAFIVPTSTSGYVVARLEDKLGQHSSDTAQINFDDCRIP
                     ADNLIGAEGEGYKIALGALEGGRIGIAAQSVGMARSAFEFALQYAKERESFGQSIFNH
                     QAVGHRLADCATQIEAARQLIWHAAALRDAGRPCLKEAAMAKLFASEMAERVCSAAIQ
                     TLGGYGVVSDFPVERIYRDVRVCQIYEGTSDVQKIIIQRA"
BASE COUNT         1284 a         2168 c         2185 g         1128 t
ORIGIN      
        1 tacagcccaa accgccctcc cacttacata gtgggagggc gttttgcttt gggggtttga
       61 tgcgtgtctc ggttcacacg cgcaagctgc ggtagcattg acgttcacgt taacgtcaag
      121 acatgccaac ctacagcatc agtgatctgg cacgggagtt tgatctgact acccgcgcca
      181 tgcgtttcta cgaagacatg ggtctattgc gccccgaacg cgcggggcca ggtgggcgca
      241 accgcgtcta tagcgggcgc gatcgggcgc ggctcaagtt gacgttgcga gccaagcgcc
      301 tgggcctgtc attgaatgag gccaaggaga tcatcgacct ctatgacagc ccacgcgaca
      361 caggggtgca gctacgcaaa ttcctggagg ttttagccat gcaccgcaag caactggagt
      421 tgcagatgcg cgacttgcag gccaatctcg acgagttgcg tgaacatgag cgtgaagcca
      481 tggatctgct cggaaaaatt gaaccaccga cggctgaaac tacagcataa ttgacgttta
      541 cgtaaacgta atgcgccagg gcttttgcgg tctgccggcg acatgtatcc atctcacacc
      601 cccttccagg agagactcca tgagcattgc cacccagttg ccaggcctga attttcagtt
      661 gggcgaagac attgacgcat tgcgcgatgc cgtgcgcgag tttgcccagt ccgagatcgg
      721 gccacgcgcg gcggagatcg atcgtgatga ccagtttccc atggacctct ggcgcaagtt
      781 cggtgacctg ggcgtgctcg gcatcaccgt gcccgaggaa tttggcggcg ccaacatggg
      841 ctatctggcg cacatggtgg ccatggaaga gatttcgcgc gccagcgcat ccgtgggcct
      901 gtcctatggc gcgcacagca atctgtgcgt gaaccagatc aaccgcaacg gcaacccggc
      961 gcaaaaggcc aagtacctgc ccaagctgat cagcggtgag catgtgggcg ccctggccat
     1021 gagcgagccg ggcgccggct ccgacgtcat cagcatgaag ctcaaggccg aggacaaggg
     1081 cggttactac ctgctcaacg gcagcaagat gtggatcacc aacggcccgg acgccgacac
     1141 cctggtggtc tatgccaaga ccgagcccga gctgggcgca cgcggcgtca cggcctttct
     1201 gattgaaaag ggcatgaagg gcttttcgat tgcgcaaaag ctcgacaagc tgggcatgcg
     1261 cggcagccac acgggcgagc tggtgttcca ggatgtggag gttccggcgg agaacgtgct
     1321 gggcatggtc aacggcggcg ccaaggtgct gatgagtgga ctggattacg agcgcgccgt
     1381 gctcaccggc ggtccgctcg gcatcatgca gtcagtgatg gacaacgtcg tgccctacat
     1441 ccacgaccgc aagcagtttg gccagagcat tggcgagttc cagcttatcc agggcaaggt
     1501 ggccgatatg tacaccgtgc tgcaggctgg gcggtcgttc gcctacaccg tggccaaaaa
     1561 tctggacatg ctgggtgccg agcatgtgcg ccaggtgcgc aaggactgcg ccagcgtgat
     1621 cctgtggacg gccgagaagg ctacctggat ggcgggcgag ggggtgcaga tttttggtgg
     1681 taatggctat atcaacgagt acccgctggg ccgcctgtgg cgtgatgcca agctgtacga
     1741 gatcggcgcc ggtaccagcg agatccgccg catgctgata ggccgtgagc tgttcgccga
     1801 aacttgctaa ggtaagcgcc ccgtaaccac caaggaagcc catgcccagc agctccatca
     1861 acgacctctt tgagcacaac cgcgcctggg cggcgcggat ggagcgcgaa aaacccggct
     1921 ttttcaccgg cctgatggcg cagcagaagc cgcgctacat gtggataggc tgctccgaca
     1981 gccgggtgcc ggccaaccag atcacgggcc tggagccggg cgaggtgttc gtgcaccgta
     2041 acgtcgccaa cgtggttgtg ccgaccgacc tcaattgcct gtccaccatc cagtacgcgg
     2101 tcgatcagct gaaggtggag cacctgatgg tcgttggcca ctacggctgc ggcggcgtgc
     2161 tggcggcgct ggaggatgtg cgcgtgggcc tggcggacaa ctggatccgc catgtaaagg
     2221 atgtgcgcga caagcatgcc gcgctgctgg acggcatcga cctgcaatgg cgccacgacg
     2281 cgctgtgcga gctcaacgcc atcgagcagg tgctgaacat cgcccattcg acggtgatgc
     2341 aggatgcctg ggcgcgcggc cagaaggtgc agatccacgg ctggtgctat ggcctgcgca
     2401 acggcctgat caccaatctg cacatgaccg tgcccggcgt gggcggtctg cacgaggtgc
     2461 accaggccgc cgtggagcag gtggcgcagc gcgcgcgcca atagcccacg ggccacggca
     2521 cagcgcaggc aggccccatg tttccgcagc ggctggacgc cccgcaggcc tttgacatcg
     2581 ccaaggcgat gctggacggc ttcaaccgcc actaccgtct gttccgtgcc gaatcggcgc
     2641 gcgccaagca ccgtttcgag acggcggact ggcacggcca gcagcgcgcg cagcgcgagc
     2701 gcatagagtt ctacgacctg cgcgtgaagg aatgtgtgcg ccggctggac aaggagttca
     2761 acgccggcgc cctgcccatg gtcgtgtggc accaggtcaa gctgcactac atcggcctga
     2821 tggtgaacca cctgcgcccc gagctggccg agaccttctt caactcggtc accaccaaga
     2881 tcctgcaccg cacgcatttc cacaacgact tcatctttgt ctggcctgcc gtcagcaccg
     2941 agtacataga aagcgaagac cccggcgcgc gccccaccta ccgcgcctac tacccggcgg
     3001 gcgatggcct gcaccaggcg gtggccgaca tcatcgagca gtttgcgctg cagcgcccgt
     3061 ttgccgacct gcggcgcgac gtggactgcg tggtgcgcgc catgcagcag cagctgggcg
     3121 ccgccaagct gcgcagcaat ttccagatcc aggtgctgac cagcctgttc tttcgcaaca
     3181 agggcgccta cgtggtgggc aagctgatca acggcttcac cgagctgccg ttcgcgctgc
     3241 ccatattgca tggcgagcag ggcacgctgg tgatcgatgc cgcgctgttc ggcgagaacg
     3301 atttgcagat gctgttcagt tttgcgcgcg cctacttcat ggtggacatg gagattccca
     3361 gcgcctacgt gcagttcctg gccacgctga tgccgcgcaa gcgccgggcc gagctctaca
     3421 tctcgctggg cctggccaag cagggcaaga cgctgtttta tcgcgacttc ctgcaccatc
     3481 tgcgctattc cagcgaccag ttccgcattg cgcccggcat caagggcatg gtgatgctgg
     3541 tcttcgacct gccgagcttt ccctatgtgt tcaagctcat caaggatcat ttcccggcgc
     3601 ccaaagacac aacgcgtgag caggtcaagg ccaaatatct gctggtcaag cagcatgacc
     3661 gcgtgggccg catggccgac acgctggaat acagtctggt ggcctttccg cgcgaccgct
     3721 tcagcgatga gctgatcgcg gaaattcaaa agcatgcgcc cagccagttg gagatcagcg
     3781 accgcgatgg tgacgggcgc caggaggtca tcatctccca cctgtacata gagcggcgca
     3841 tgatcccgct gaacatccac ctgcaggaat gcctggatgc cggcctggac gaacccgagt
     3901 cagccagcgc gctggagcat gccgtgctcg aatacggcaa cgccatcaag gatctggtgg
     3961 ccgccaacat cttccccggc gacatgctgt ggaagaactt cggcatgacg cgcaacggca
     4021 aggtggtgtt ctacgactac gacgagatcg aatacctcac cgactgcaac ttccgccgcg
     4081 tgcccgcgcc gcgctgcgag gaggatgaac tgtccggcga ggtctggtac gcggtcggcc
     4141 cgcacgatgt cttccccgaa accttcggcc ccttcctgct cggcaacgac gccgtgcgtg
     4201 ccgcctttat gcgccaccat gcggatctgc tggacgtgga tttctggcag tcgcacaaag
     4261 agcgcataca ggccggccat gtctatgacg tgtttcccta cgacagcacg cggcgctttg
     4321 gcggcgtgcc gttaccaacc caacccccag gagatatcac atgagccaac accaagaccc
     4381 cgtcgtcatc gtcagcgccg cgcgcacgcc catgggcggc ttcatgagcg acttcgccga
     4441 cctggccgcg catgacctgg gcggcgtcgc catcaaggcc gccgttgagc gcgccggtat
     4501 tgcgcccgcg ctggtcgagg aggtgctgtt cggcaactgc ctgatggccg gccagggcca
     4561 ggcgccggcg cgccaggcgc ttttcaaggg cgggctgccg caaagcacgg gcgcggtgac
     4621 cctgtccaag atgtgcggcg ccggcatgga ggccaccatc ctggcccatg accagctgat
     4681 cgccggcagc cgcgaggtga tggtctccgg cggcatggag agcatgagcg gcgcacccta
     4741 cctgctgaag aagggccgcg gcggctaccg catgggccac gacaaggtgt tcgaccacat
     4801 gatgctcgac ggcctggaag acgcctacga gcccggccgc tccatgggca cctttggcga
     4861 ggattgcgcc gccaagtacc agttcacgcg cgagcagcag gacgcctttg ccatcgccag
     4921 cgtcacgcgc gcacaggagg ccgccaggag cggcgccttc gacgccgaga ttgccccggt
     4981 tacgctcaag acgcgcaagg gcgaggtcac ggtcagcgtg gacgaaggcc cggccaaggc
     5041 ccgcctggac aagatcgcca gcctcaagcc cgccttcagg aaggacggca ccatcaccgc
     5101 agcgtccagc tccagcatca acgatggcgc cgccgccatg gtgctgatgc gcgcgtccac
     5161 cgcccagcgg ctgggctgca agccgctggc gcgtatcgtc ggccatgcca cccatgccca
     5221 ggcacccgaa tggttcagca ccgccccggt gggtgcgacc gagaagctgc tgaagaaaat
     5281 cggctggcag gtgggcgacg tggatctgtg ggagatcaac gaggccttcg ccgtcgtgcc
     5341 catggccctg atggcggatc tgaagattcc ccacgacaag gtcaacgtca acggcggcgc
     5401 ctgcgcgctg ggtcacccca taggcgccag cggcgcgcgc attttggtca cgctggtgca
     5461 tgcgctcaag gcccgcggcc tgcagcgcgg cctggccacg ctgtgcatag gcggcggcga
     5521 ggccacggcc atggccatcg agctgatctg aggattgctc atttttcaat agctgttggc
     5581 gcttattcca tgggcgctgg aggcattttt ggccacaagc cactattttg cgagagcacg
     5641 accatgctgc tgacccaaga ccaggaaatg atccgcgacg ctgtgcgtgc gttcgcccag
     5701 gaagagctct ggccccacgc cgcgcgctgg gacaaggagc accactttcc gcgcgacgcg
     5761 caccagggcc tggccgcctt gggcgcgtat ggcatctgcg tgcccgagga tctcggcggc
     5821 gccgggctgg actacctgtc gctggccctg gtgctggaag aaatcgccgc cggcgatggc
     5881 ggcaccagca ccgccatcag cgtcaccaac tgcccggtca acgccatcct catgcgctac
     5941 ggcaatgcgc ggcaacagcg cgactggctt gcccccctgg cgcgcggcga gatgctgggc
     6001 gcgttctgcc tgaccgagcc gcatgtgggc tcggacgcct ccgcgctgcg caccacggcg
     6061 gtgaggcagg gcgacgaata cgtcatcaac ggcgtcaagc agttcatcac cagcggcaag
     6121 aacggccagg tggccatcgt catcgccgtc accgacaagg gcgcgggcaa gaagggcatg
     6181 agcgccttca tcgtgcccac cagcacgtcc ggctatgtgg tggcgcggct ggaggacaag
     6241 ctgggccagc acagcagcga cacggcgcag atcaacttcg acgactgccg catcccggcg
     6301 gacaacctga tcggcgccga gggcgagggc tacaagatcg ccctgggcgc gctggagggt
     6361 gggcgcatag gcatcgccgc gcaaagcgtg ggcatggcgc gtagcgcctt tgaattcgcc
     6421 ctgcaatacg ccaaggagcg cgagagcttc ggccagtcca tcttcaacca ccaggccgtg
     6481 ggccatcgcc tggccgactg cgccacgcag atcgaggccg cacgccagct catctggcac
     6541 gccgccgccc tgcgcgacgc gggccgcccc tgcctgaagg aggcggcgat ggccaagctg
     6601 ttcgccagcg agatggcgga acgcgtgtgc agcgccgcca tacagacgct gggcggctac
     6661 ggcgtggtga gcgacttccc tgtggagcgt atctaccgcg acgtgcgcgt gtgccagatc
     6721 tacgagggca cgagcgacgt gcagaagatc atcatccagc gcgcg
//
LOCUS       DAOMTQ010000156         6745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2610_length_6745_cov_32.456203, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000156 DAOMTQ010000000
VERSION     DAOMTQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_6745_cov_32.456203"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PK518_12945"
     CDS             <1..510
                     /locus_tag="PK518_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRM49862.1"
                     /translation="PRVLGYSFKPVSFWYCHRQDGSLRAIVAEVNNTFGERHAYLLDH
                     PHYGQELRARKAFHVSPFCAIDGGYRFEFRRGGADGLQSARVRIDYHDAEGPVLLTGM
                     AGRLEPLTAASRRRALWRYPLLTLGVMARILWNALLLWLKQVPFHTKPQAPAMPVSRS
                     SSRSSSSDH"
     gene            513..1733
                     /locus_tag="PK518_12950"
     CDS             513..1733
                     /locus_tag="PK518_12950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRM49863.1"
                     /translation="MNTTTTAFQLSLPAGLPAPARNGLRLLQRLQHGTLHLELPDGST
                     LQLGQGSQPHASLRLHDWRVFGAVLRSGDIGLAEGYIDQHWSTPHLGDLLRLLMANRE
                     ALESLVYGAWWGRLAYRLRHLLHRNTRTGSRRNIQAHYDLGNAFYQLWLDPSMNYSSA
                     WFQGDLAGDLTAAQHAKVRRALHSAGVQAGSRVLEIGCGWGALAEMAAGEFQAHVTGV
                     TLSNEQLAFGQQRLQRAGLAARAELRLQDYRDIQDAPFDAICSIEMVEAVGQAYWPSY
                     FGTVARLLKPGGRACVQSIVIDDALFERYVQGTDFIQQYVFPGGCLPSPARFRAAAQQ
                     AGLRVVEELAFGADYAETLRRWHRQFTQQHAQVLAQGFDAKFLRTWEFYLAYCEAAFD
                     SGSIDVVQYTLVKN"
     gene            1832..2371
                     /locus_tag="PK518_12955"
     CDS             1832..2371
                     /locus_tag="PK518_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRM49864.1"
                     /translation="MGVAVASAGPGVSAADASAPPAFIHQALPDATLWGQGQMRFFGL
                     RIYDARLWAGLQFEAADFGAYALALELTYHRAFTGAAIAQRSIEEIERQGDLAPAQAQ
                     RWQRALAAVLPDVQPGERLTGLYQPGLGMRLWRGQQALGAIDDAELARRFFGIWLSPR
                     TSEPGLRSALLARKPGAGP"
     gene            2368..3687
                     /locus_tag="PK518_12960"
     CDS             2368..3687
                     /locus_tag="PK518_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49865.1"
                     /translation="MTHPLSWNPSQVLPWRAGLAYGLLGLPLAFAALPLYVVLPHYYA
                     SNLGLPLASVGALLLLVRLIDAGAEPLLGRLSDRLYRRTAHTVLWVVGASALLLGGGM
                     AGLFFPPVQGPGALAAWMVAGLLFTCLAHSLLVIAHQAWGVRLGGDTVQRSRIVAWRE
                     GPGLIGVVTASALSVAWGAGTMLGVFALALLAGWLALWQAPRPPFSAAPQEGHEGREP
                     RYRQLWQPLTQAPFRRLLAVFLCNGIASAIPAALMLFFAQDRLQATSPQIALFLVLYF
                     VSGALSMPGWLRVVRRFGLERAWLAGMLLAVACFGWAAALQGGQVAAFAVICVLSGAA
                     LGADLAVPGALLALLIERHGAQGTNDGAYLGWWNLATKLNLALAAGATLPLLQWLGYM
                     PGSQDPLALQRLAWIYALLPCALKLMAAWLLHRLLITSRNPPQGVTP"
     gene            3684..4220
                     /locus_tag="PK518_12965"
     CDS             3684..4220
                     /locus_tag="PK518_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HRM49866.1"
                     /translation="MNTRRHVLLALLAGSAVLAGCASPQVSDYAQQRPKLELDRYFNG
                     RIRAHGIFQKRGGEVVRRFTVVMDCHWEGNQGVLDEAFSYSDGSTQRRVWRLAKLADG
                     RYTGRADDVVGEAKGQESGNAFRWNYTLRLPVDGKEYDVQFDDWMFLVDERVMLNRAT
                     MSKFGITLGEVLLSFTKD"
     gene            4225..5007
                     /locus_tag="PK518_12970"
     CDS             4225..5007
                     /locus_tag="PK518_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM49867.1"
                     /translation="MPLNPPITDWRGQRVWLVGASSGIGRAVASLLHARGAQVCVSAR
                     QQALLDAFVREHPGSQALALDVTDAQAVASAAQRLLAGGPLDLVCYCAGHYRPMSAHA
                     LDLPELLQHQSVNVTGALQVLAAVVPALLQSAGAGRTPHLSLVASVAGWRGLPQSLAY
                     GPTKAALINLAENLFLDLRPHGVGVTLVNPGFVDTPLTAQNQFAMPALLTPEQAALAI
                     VRGWQRGQFELHFPKRFTGVLKLMRCLPYRWYFPLVHRFTGL"
     gene            5010..5525
                     /locus_tag="PK518_12975"
     CDS             5010..5525
                     /locus_tag="PK518_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRM49868.1"
                     /translation="MRVSTPIVCGDTQATAQAATQRLIALYEQLSPEHLATLDAYYAS
                     DAHFKDPFNDVRGVPAIAQIFAHMFATLDQPRFAVTQHIVQGDQAFLGWEFRFRMRRW
                     RPQVEQCIHGATLVRFDAQGRVALHRDYWDAAQELYEKLPVLGGLMRWLRRSASATST
                     TKVSHAHRPSP"
     gene            5503..6192
                     /locus_tag="PK518_12980"
     CDS             5503..6192
                     /locus_tag="PK518_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRM49869.1"
                     /translation="MLTVHHLETSRSQRILWLLEELAVPYDIRLYRRDPVSRLAPAAL
                     KQIHPLGKSPVVTDGDTVVAESGAIIEYLVERFGAQARNELAQLEPARGTPEHRQCRF
                     WMHYAEGSLMNWLVMKLVFDTIPRQPMPFFVRPIARALCSKVQQKLIHPNVQTALAFI
                     DGHLAKNRWFAGEHLSMADFQMSFAVEAALARGGDESQWPHLVAYRQRMQERPAYQRA
                     LDKGGPVLMQD"
     gene            6205..>6745
                     /locus_tag="PK518_12985"
     CDS             6205..>6745
                     /locus_tag="PK518_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRM49870.1"
                     /translation="MTAPAHTDTQQPPARATSALVWLRRDLRCGDHAALYHALRRYPR
                     VYCAFVFDTDILDALPNHGNQRQDRRVEFIHASVLELHDGLQRLAAQSGAPGGGLIVR
                     HGPALPCIVQLAQELGVQEVLVNRDYEPQAIARDQHVAQALHALGIAFSDYKDQVLLD
                     RDEVLTQQGRPYSVFTPYKR"
BASE COUNT          968 a         2322 c         2283 g         1172 t
ORIGIN      
        1 ccgcgcgtgc tgggctacag cttcaagccg gtgagctttt ggtactgcca ccgccaggac
       61 ggcagcctgc gcgccatcgt ggccgaggtg aacaacacct ttggcgagcg ccacgcctac
      121 ctgctcgacc acccgcacta cggccaggag ctgcgggcgc gcaaggcctt ccacgtctca
      181 ccgttttgcg ccatcgatgg cggctaccgc tttgagtttc gccggggtgg ggccgacggc
      241 ctgcaatcgg cgcgcgtgcg catcgactac cacgacgccg agggccccgt gctgctcacc
      301 ggcatggccg ggcggctgga gccgctcacc gccgccagcc gccgccgcgc gctgtggcgc
      361 taccccctgc tgacgctggg cgtgatggcc cgcattctct ggaacgcctt gctgctgtgg
      421 ctcaagcagg tgccgttcca caccaagccc caggcgccgg cgatgcctgt gtcccgttca
      481 tcttcccgtt catcttcatc cgaccactga ccatgaacac cacgaccact gcgtttcaac
      541 tttcgttgcc tgccggcctg ccggcccctg cgcgcaacgg cctgcgcctg ctgcagcgcc
      601 tgcagcacgg cacgctgcac ctggagctgc ccgacggcag cacgctgcaa ctgggccagg
      661 gcagccagcc gcacgccagc ctgcgcctgc acgattggcg ggtgtttggc gcggtgctgc
      721 gctcgggcga catcggcctg gccgagggct atatcgatca gcactggagc acgccgcacc
      781 tgggtgactt gctgcgcctg ctgatggcca accgcgaggc actggagtcg ctggtgtacg
      841 gcgcctggtg ggggcggctg gcctaccggc tgcgccatct gctccaccgc aacacgcgca
      901 ccggcagccg gcgcaacatc caggcccact acgacctggg caacgccttc taccagttgt
      961 ggctggaccc gagcatgaat tactcgtccg cctggttcca gggcgatctg gcgggcgatt
     1021 tgaccgccgc gcagcatgcc aaggtgcgcc gcgcgctgca cagcgccggc gtgcaagcgg
     1081 gtagccgggt gctggagatt ggctgcggct ggggcgcgct ggccgagatg gcggcgggcg
     1141 agttccaggc ccatgtgacc ggcgtgacgc tctccaacga gcaactggcg tttggccagc
     1201 agcgcttgca gcgcgccggg ctggcggccc gggccgagct gcgcctgcag gactaccgcg
     1261 acatccagga cgcgccgttc gacgccatct gctccatcga aatggtcgag gccgtgggcc
     1321 aggcctactg gcccagctac tttggcaccg tggcgcgctt gctcaaaccg ggcgggcgcg
     1381 cctgcgtgca aagcatcgtc atcgacgacg cgctgttcga gcgctatgtg cagggcacgg
     1441 acttcatcca gcagtacgtc ttccccggcg gctgcctgcc cagcccggcg cgctttcgcg
     1501 cggcggcgca gcaggccggt ctgcgcgtgg tggaggagct ggcctttggc gccgactacg
     1561 ccgagacctt gcgccgctgg caccgccagt tcacccagca gcatgcccag gtgctggcgc
     1621 agggttttga tgcgaagttc ttgcgcacct gggagtttta cctcgcgtac tgcgaagccg
     1681 ccttcgacag cggcagcatc gatgtggtgc agtacacatt ggtgaaaaat taaaaaatga
     1741 tagctgccag cgcttgatgc atatgcctta ccgcctgatt tcaccattga tttacctggc
     1801 gagagtccgc gtgctggccg cagcggtggc catgggggtg gctgttgcca gcgcgggccc
     1861 tggcgtatcg gcagcggacg catcggcgcc gcccgccttc atacaccagg cgctgcccga
     1921 tgcaacgctg tggggccagg ggcagatgcg atttttcggg ctgcgcatct acgacgcgcg
     1981 cctgtgggcc ggcctgcagt ttgaagcggc cgactttggc gcctacgccc tggccctgga
     2041 gctgacctac caccgcgcct ttaccggcgc cgccattgcc cagcgctcca tcgaggagat
     2101 cgagcgccag ggagatctgg cgccagcgca ggcccagcgc tggcaaaggg cgctggccgc
     2161 cgtactgccc gacgtgcagc cgggcgagcg cctcacgggc ctgtaccagc ctggcctggg
     2221 catgcgcctg tggcgcggcc agcaggccct gggtgcgatt gacgacgccg agctggcacg
     2281 ccgctttttc ggcatctggc tgtcgccacg cacgtccgag cccggcctgc gcagcgcctt
     2341 gctggcgcgc aagccaggag ccgggccatg acccacccct tgtcatggaa cccctcgcaa
     2401 gtgctgccct ggcgcgccgg gctggcctac gggctgctgg gtctgccgct ggcttttgcc
     2461 gcgctgccgc tgtacgtggt gctgccgcac tactacgcca gcaaccttgg cctgccgctg
     2521 gccagtgtgg gggcgctgct gcttctggtg cggctgatcg atgccggggc cgaaccgctg
     2581 ctcgggcgcc tgagcgaccg gttgtaccgc cgcaccgcgc acaccgtgct gtgggtagtt
     2641 ggcgcatcgg cgctgctctt gggcggcggc atggcggggc tgttttttcc gccggtgcag
     2701 gggccggggg cactggcggc gtggatggtg gcgggcctct tgttcacctg cctggcgcac
     2761 agtctgctgg tcatcgccca ccaggcctgg ggggtgcgcc tggggggtga caccgtgcag
     2821 cgcagccgca tcgtggcctg gcgcgagggg ccgggcctga tcggcgtggt cacggccagc
     2881 gcgctctcgg tggcctgggg cgcgggcacc atgctgggcg tgttcgcact ggcattgctg
     2941 gccggttggc tggcgctgtg gcaggcgccg cgtccgccgt tttcagccgc gccgcaggag
     3001 gggcatgagg gacgggagcc gcgctatcgc caactttggc agccgctgac ccaggcgccc
     3061 ttccgccgtc tgctggcggt gtttctgtgc aatggcatcg ccagcgccat accagccgcg
     3121 ctgatgttgt tcttcgccca ggatcggctg caggctacgt cgccgcagat tgcgctgttc
     3181 ctggtgctgt atttcgtcag cggtgcgctg tccatgcccg gttggctgcg ggtggtgcgc
     3241 cgcttcgggc tggagcgggc gtggctggcc ggcatgttgc tggcggtggc ctgctttggc
     3301 tgggcggccg cgctgcaggg cgggcaggtg gcggcatttg ccgtgatctg cgtgctgtcg
     3361 ggcgcggcgc tgggcgccga cctggccgtg cccggcgcac tgctggcctt gttgatcgag
     3421 cgccacggcg cccagggcac gaatgacgga gcctacctgg gctggtggaa cctggccacc
     3481 aagctcaacc tggcgctggc cgccggggcc acgctgccgc tgctgcaatg gctgggctac
     3541 atgccgggca gccaggatcc gctggcactg caacgcctgg catggattta tgcgctgctg
     3601 ccgtgcgcgc tcaagctgat ggcggcctgg ttgctgcatc gcctgttgat cacctctcga
     3661 aacccacccc aaggagtgac accatgaaca cacgccgcca tgtcttgctt gccctgctcg
     3721 cgggctcggc tgtgctggcc ggctgcgcca gcccccaggt gagcgactac gcgcagcagc
     3781 gccccaagct ggagctggat cgctatttca atggacgcat ccgcgcccac ggcatttttc
     3841 aaaaacgcgg cggcgaggtg gtgcgccgct tcaccgtggt gatggactgc cactgggagg
     3901 gcaaccaggg cgtgctggac gaggccttca gctattccga cggcagcacc cagcgccgcg
     3961 tctggcgcct ggccaaactc gcggatggcc gctacaccgg ccgtgccgat gacgtggtgg
     4021 gcgaggccaa ggggcaggaa tctggcaacg ccttccgctg gaactacacg ctgcgcctgc
     4081 cggtggacgg caaggagtac gacgtgcagt ttgacgactg gatgttcctg gtcgatgagc
     4141 gggtgatgct caaccgcgcc accatgagca agtttggcat cacgctgggc gaggtgctgc
     4201 tgtctttcac caaggactaa cgccgtgccc ctcaaccccc ccatcaccga ctggcgcggc
     4261 caacgcgtgt ggctggtcgg tgcgtccagc ggcatcgggc gcgcggttgc gtcgctgctg
     4321 cacgcacgtg gcgcgcaggt gtgcgtgtcg gcgcgtcagc aggccttgct cgatgccttc
     4381 gtgcgcgagc accccggcag ccaggcgctg gcgttggatg tgaccgacgc gcaggccgtc
     4441 gccagcgcgg cgcagcggct gttggcgggc ggcccgctgg acttggtctg ctactgcgcc
     4501 ggccactacc gccccatgtc ggcccacgcg ctggacttgc ccgagctgct gcagcaccag
     4561 agcgtcaacg tcacgggcgc gctgcaggtg ctggccgccg tggtacccgc gctgctccag
     4621 tccgccggcg ccgggcgcac gccgcacctg agcctggtgg ccagcgtggc cggctggcgt
     4681 ggcctgccgc agagcctggc ctatggcccc accaaggcgg cgctgatcaa cctggcggaa
     4741 aacctgtttc ttgatctgcg cccacacggt gtgggcgtca ccctggtcaa ccccggtttc
     4801 gtcgataccc cgctgacggc gcaaaaccag tttgccatgc cggcactgct cacgcctgag
     4861 caggcggcgc tggccatcgt gcgcggctgg cagcgggggc agttcgagct gcactttccc
     4921 aagcgcttca cgggcgtgct caagctcatg cgctgcctgc cgtaccgctg gtatttcccg
     4981 ctggtgcacc gtttcacggg gctttgagca tgcgcgtttc caccccgatc gtttgcggcg
     5041 acacccaggc cacagcgcag gccgccaccc agcggctgat tgcgctttac gagcagctct
     5101 cgcccgagca cctggccacg ctggacgcct attacgcgag cgatgcgcac ttcaaagacc
     5161 ccttcaacga cgtgcgcggc gtgcccgcca tcgcgcagat ttttgcgcac atgttcgcca
     5221 cgctggatca gccccgcttt gccgtcaccc agcacatcgt gcagggcgac caggcctttc
     5281 tgggctggga gttccgcttt cgcatgcgcc gctggcgccc gcaggtggag caatgcatcc
     5341 acggtgccac cctggtgcgc tttgacgccc agggccgcgt ggcgctgcac cgcgactatt
     5401 gggatgccgc ccaggagctg tacgagaaac tgcccgtgct gggcggcctg atgcgctggc
     5461 tgcgccgatc cgcctcggcg acttcaacca ccaaggtgtc ccatgctcac cgtccatcac
     5521 cttgaaacgt cgcgctcgca gcgcatcctc tggctgctcg aagagctggc cgttccctat
     5581 gacatccgcc tctaccggcg cgacccggtg agccgactgg cgcctgcggc gctcaagcaa
     5641 atccaccccc tgggcaagtc gcccgtggtg accgatggcg ataccgtggt ggccgaatcg
     5701 ggcgccatca tcgaatactt ggtggaacgc tttggcgccc aggcacggaa cgaactggcc
     5761 cagctggagc cggcgcgggg gacacccgaa catcgccagt gccgcttctg gatgcactac
     5821 gcggaaggct cgttgatgaa ctggctggtc atgaagctgg ttttcgacac cattccgcgc
     5881 cagcccatgc cgttttttgt gcgcccgatc gcacgcgccc tatgcagcaa agtgcagcaa
     5941 aaactcattc accccaacgt tcaaaccgcg ctggcgttca tcgatgggca tttggccaag
     6001 aaccgctggt ttgccggcga gcacctgagc atggccgatt ttcagatgag ctttgccgtt
     6061 gaggccgccc tcgcgcgggg cggcgacgag tcccagtggc cgcacctcgt tgcctaccgc
     6121 cagcgcatgc aggaacggcc ggcttaccag cgagcgctgg acaagggcgg gccggtgttg
     6181 atgcaagact gaagcccaac caagatgaca gccccagccc acaccgacac gcaacagccg
     6241 cctgccaggg caacaagcgc cctggtctgg ctgcgccgcg acctgcgctg cggcgaccat
     6301 gccgcgctgt accacgcgct gcgccggtat ccgagggtgt attgcgcctt cgtgttcgat
     6361 accgacatcc tggacgcgct gcccaatcac gggaatcagc gccaggatcg ccgtgtggaa
     6421 ttcatccacg ccagcgtgct ggagctgcac gacggcctgc agcggctggc cgcgcaaagc
     6481 ggcgcgcccg gcggcgggtt gatcgtgcgc catggccccg cgctgccatg catcgtgcag
     6541 ctggcgcaag agctgggcgt gcaggaggtg ctggtcaatc gcgactacga gccccaggcc
     6601 attgcccgcg accagcacgt ggcccaggcc ttgcatgcgc tgggcattgc cttcagcgac
     6661 tacaaggatc aggtgctgct cgaccgggac gaggtgctga cccagcaggg ccggccctac
     6721 agcgtgttca cgccctacaa gcgcg
//
LOCUS       DAOMTQ010000157         6669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2650_length_6669_cov_24.610221, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000157 DAOMTQ010000000
VERSION     DAOMTQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_6669_cov_24.610221"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1329)
                     /locus_tag="PK518_12990"
     CDS             complement(286..1329)
                     /locus_tag="PK518_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HRM49871.1"
                     /translation="MTRLVDLPTLAALVRGMGPERFLCELADEIQASFLRWDDYDKSA
                     RLASHSPTGVIELMPVADERLYSFKYVNGHPGNPALGLPTVMAFGMLAEVATGLPLLL
                     SELTLATALRTAATSALAARQLARPGSRSMALIGNGAQAEFQALAFHALLGIDELRVF
                     DIAPAASERLQRNLATVPGLTLRRAASVIQAVRGADIVTTATASKSHACIITAAMLEP
                     GMHLNAVGGDCPGKTELHPDVLRAARIVVEYPPQTRVEGEIQQLPADHPVAELWRVLA
                     GQAPGREHEGQVTLFDSVGFALEDHATLRLIHRLAEAHHLGIYLELVPGQGDPKDLFG
                     RVRTTGQQSPTPS"
     gene            complement(1396..2223)
                     /locus_tag="PK518_12995"
     CDS             complement(1396..2223)
                     /locus_tag="PK518_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM49872.1"
                     /translation="MQPLALWPGPTRQHIFGVLTDIDDTLTTNGAITPDALAALADLK
                     AAGLHVIAITGRPVGWSEPFAASWPVDSIVAENGAVALLPQDTRKNSLERPPIKREQL
                     SKIYQQDAAARAANYARMQQVLADIEARVPGARRATDSAGRETDIAIDHSEFTQLPQA
                     AIDACVAIMRAAGMMATVSSIHINGWYGAHNKLEGARWIVRELLGRDLDAELSHWVYV
                     GDSTNDQLMFEHFENSVGVANIARFVPQLTHLPRYVTQGERGAGFAELARAILAARP"
     gene            complement(2274..>2975)
                     /locus_tag="PK518_13000"
     CDS             complement(2274..>2975)
                     /locus_tag="PK518_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49873.1"
                     /translation="VAVSQGVRHQVAQVEFVIVPRDQGRVRSTKPAQLSGLKDAKQLP
                     GVRSWLDMPRPLQDLYYNPIARDWNKYRESQVYGFLQAFHDRALKAGLPKSQLFSHQI
                     VPDVNSSWNPILFAAGTTLEGSAPWQHGLNMYGGATNSTWLRSFTERHKITDYGAPEF
                     NPQQWKRDGVHLAALQSRLDAGARFISPYYFSVIPDRFKGGVEHGVNRMEIRPDNTKD
                     GSDKFFHAIIEFAKR"
     assembly_gap    2976..3075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3076..3629)
                     /locus_tag="PK518_13005"
     CDS             complement(<3076..3629)
                     /locus_tag="PK518_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49874.1"
                     /translation="MISSLTRAVALLSFSLATAMASAQPEAQGQRRPMIISPTVDGML
                     LCDEAAKNTAITNIDAAYAYCGQRKLDGAAAITRLLNTLEPGGAKGAVQVGYTATLQL
                     LGLFQKTSKGWAIDKERVDTFLRVISKVDRPVVLYFSADHFDSIGPLAEELRGDPVNL
                     MQLRDGKPLELGYFGYRIIPYTLSP"
     gene            complement(3673..4659)
                     /locus_tag="PK518_13010"
     CDS             complement(3673..4659)
                     /locus_tag="PK518_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM49875.1"
                     /translation="MRIAVAIPCYKVTRHVLEVIQAIGPEVEAIYAVDDACPDGSGRL
                     IEEHNRDPRVRVLYNPENRGVGGAIVTAYQAAMADGMDIVVKIDGDGQMDPALLPSFV
                     RPLLQGRADYTKGNRFFRPESVQGMPPVRLFGNAMLSFLTKLSCGYWNVMDPTNGYTA
                     VRTSVLAELPLDKLEQRYFFETDMLFRLNTVRAVVKDIPMDSVYANEESNLKVGRVLP
                     EFLKKHISRLWRRYVYNYLVRDFNVGTLYSLSGVLLLLAGTAFGTWHWVSGNFNSHPA
                     TSGTVMLAALPVLIGIQSLIAFLHYDVSNVPTEPLSLSLDHSALPPNDLSVS"
     gene            complement(4672..5019)
                     /locus_tag="PK518_13015"
     CDS             complement(4672..5019)
                     /locus_tag="PK518_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49876.1"
                     /translation="MSIPLGLYTLLCVFGISIGQLLFKKASAALPATLSLIGLLQNGW
                     LMAALVLYGITTLGWVWILRHAPLYLAYPFMGLAFLIVPTLAWAFLGEPLHWRTLAGG
                     ALIMAGVALASTS"
     gene            5488..6582
                     /locus_tag="PK518_13020"
     CDS             5488..6582
                     /locus_tag="PK518_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49877.1"
                     /translation="MHTVHLTAVFANGQRVTLKGATTDVPTVVIQEIKRRHWRALALV
                     LLSVMVIVYALRRWGSEGLAWQQRWLDGNRMAWGIGAVFVLLVATGVTGSSMGVLFNS
                     PYGESVLEVQGASRTLLGDEGVRGDEWGVQLPNLLAQLHHEPRFPVINGLLGEGGQNM
                     GVIGMTGVPVQQWAALARPATWGYFVLPLRQAMAWHWQLPFWGCLLALWWMLNVLRPA
                     QRGLSLALSLAFCLAPYAAAWSNWPLYATLFPALAFVVATYLLRCTGFWMGAVLGAVL
                     GWLLACWCLVLYPAWIIVVGSAMAFIGLGWWADNRERIRFGSVQLVALVVSAAILGAL
                     LGSWWLDTKDAIALMRATVYPGGRGAMPGG"
BASE COUNT         1175 a         1864 c         2216 g         1314 t
ORIGIN      
        1 atgagttttc tgtccatcta tgtctccgtg gtgataaatg acgtggtacg tgatcggccg
       61 gtcactggtg atccggcggc gttgctgacg caagtaccgt gcctgtcatt tgcactgccg
      121 caatggcttg ctgtaccgag acatatgctc cgaacattgg catacgagtt cagaaacgga
      181 gcaggctgcc ccacggccag ccaaggctgg cgcagtccgg gccaggccct gggcgtcata
      241 tcagggcgca agccggtgta tcgcgcgctg gtatggcgtg gtgcttcaag agggggtggg
      301 cgactgctgg cctgttgtgc gcacacggcc aaacaagtcc ttggggtcgc cctggccggg
      361 caccagctcc aagtaaatgc ccaggtggtg cgcctcggcc agccgatgga tgaggcgcag
      421 ggtggcatgg tcttcgagcg caaagccgac cgagtcgaac agcgtcacct gcccctcgtg
      481 ctcgcgtcct ggcgcctggc ctgcgagcac ccgccacagc tcggcgaccg ggtggtcggc
      541 gggcagctgc tggatctcgc cctctacgcg ggtttgcggc gggtattcga caacgatgcg
      601 cgctgctcgc agtacatccg ggtgcagctc ggtcttgccc gggcagtcgc cgcccacggc
      661 attcaggtgc atgccgggct ccagcatggc ggccgtgatg atgcaggcgt ggctcttgct
      721 ggccgtggcc gtggtcacga tgtcggcgcc gcgcacggcc tggatcaccg atgcggcgcg
      781 ccgcagcgtc agcccaggca ccgttgccag gttgcgctgc agccgctcac tggccgccgg
      841 ggcgatgtcg aacacgcgca gttcgtcgat gcccagcagc gcgtgaaagg ccagggcctg
      901 gaactcggcc tgcgcgccgt tgccgatcag cgccatgctg cggctgcccg gccgcgccag
      961 ctgccgcgcc gccagggccg aggtggcggc ggtgcgcagg gccgtggcca gggtcagctc
     1021 ggacagcagc agcggcaggc ccgtggccac ctcggccagc atgccgaagg ccatcaccgt
     1081 gggcaggccc aacgccgggt tgccggggtg gccgttgaca tacttgaagc tgtagagccg
     1141 ctcgtccgcc acgggcatga gctcgatcac gccagtcggg ctgtggctgg ccaggcgtgc
     1201 cgatttgtcg tagtcgtccc aacgcaggaa gctggcctgg atctcgtcgg ccagctcgca
     1261 gaggaagcgt tctgggccca tgccccgcac cagggcggcc agggtcggaa gatcgacgag
     1321 tcgggtcatg gggcgatcct gtggcgagcc gggatgccgc cattgtggac ggccccacgc
     1381 cgccgggcaa accgcctacg gcctggcggc caggatcgcg cgcgccagct cggcaaagcc
     1441 cgcgccgcgc tcgccctggg tcacgtagcg cggcaggtgc gtgagctgcg gcacgaagcg
     1501 cgcgatgttg gccacgccca cgctgttctc gaaatgttcg aacatcagct ggtcattggt
     1561 cgagtcgccc acatagaccc agtgcgagag ctcggcatcc agatcgcgcc ccagcagctc
     1621 gcgcactatc cagcgcgcgc cttccagctt gttgtgcgcg ccataccagc cgttgatgtg
     1681 gatgctgctc accgtggcca tcatgcctgc ggcgcgcatg atggccacgc aggcgtcgat
     1741 ggccgcttgc ggcagctgcg tgaactcgct gtggtcgatg gcgatgtcgg tctcgcgccc
     1801 ggccgagtcg gtggcgcgcc gcgcaccggg cacgcgcgcc tcgatgtcgg ccagcacctg
     1861 ctgcatgcgc gcgtaattgg cggcgcgtgc ggcggcgtcc tgttgatata tttttgatag
     1921 ctgttcgcgc tttattggtg ggcgctcaag gctgtttttc ctagtatcct gcggcaacag
     1981 cgccacagcc ccgttctcgg ccacgatgct gtccaccggc cagctggcgg caaacggctc
     2041 gctccagccc acgggccggc cagtgatggc gatcacatgc aggccggcgg ctttcaggtc
     2101 ggccaacgcc gccagcgcgt cgggtgtgat ggcgccgttg gtggtcaggg tgtcgtcgat
     2161 gtcggtgagc acgccgaaga tgtgttggcg tgtcgggcca ggccacaggg caaggggttg
     2221 catagagggg cgtgtagtga aagccgttgc tagtccgcca aggtcgtgga tgctcaacgt
     2281 tttgcgaatt caatgatggc gtgaaagaac ttgtcggagc catccttggt gttgtcgggg
     2341 cggatctcca tgcggttgac cccatgctcc acgccgccct tgaatctgtc gggaataact
     2401 gaaaagtagt atgggctgat gaagcgtgcg ccggcatcga ggcgtgactg cagggccgcg
     2461 agatgcacgc cgtcgcgctt ccactgttgt ggattgaact caggagctcc gtagtcggtg
     2521 attttgtgac gctccgtaaa gctgcgcagc caggtactgt tggtggcacc tccgtacata
     2581 ttcaggccat gctgccacgg ggcactacct tccagggtgg tgcctgctgc aaaaagtatc
     2641 gggttccatg aagagttgac gtcgggaacg atctggtgtg aaaacaattg ggactttggt
     2701 agccccgcct tcagcgcccg gtcatggaat gcttgcagaa agccgtacac ctgtgattcg
     2761 cgatacttgt tccagtcccg tgcaatcggg ttgtagtaca ggtcttgcaa agggcgcggc
     2821 atgtccagcc atgaacgtac cccgggtagt tgcttggcgt ccttcagccc agaaagctgt
     2881 gcaggtttgg tgctgcgaac gcggccctgg tcacgaggca cgatcacgaa ttcaacctgg
     2941 gcaacttgat ggcgtacgcc ttgagagacg gcgacnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnggtga cagcgtgtag ggaatgatgc gataaccgaa gtagcccagc
     3121 tccagcggtt tgccgtcgcg cagttgcatg aggttgacgg ggtctccacg cagttcctca
     3181 gcaagcgggc cgatggagtc gaagtgatcc gccgagaaat acaggacgac ggggcgatct
     3241 accttgctga tcacgcgtag aaaggtgtcc acccgttctt tatcgatagc ccaccctttg
     3301 gacgttttct ggaacagtcc cagcaattgc agcgtggccg tgtagccgac ctgcaccgca
     3361 cccttggcgc ctcccggctc cagcgtgttc agtaggcgtg tgatggcggc agcgccatcg
     3421 agcttgcgct ggccacaata ggcatatgct gcgtcaatgt tcgtgatggc agtgttcttt
     3481 gcggcctcgt cgcacaacag catgccgtcc accgtagggg agatgatcat cggcctgcgt
     3541 tgcccctgcg cttcgggttg agcggatgcc attgctgtgg cgagtgaaaa gctgagcagg
     3601 gccacggccc gggtcaaaga ggaaatcatt cgggctccac cgagcgcagc agtgaagctg
     3661 ccaccatatg ttttatgaaa cactgagatc gttgggtgga agcgccgaat gatccagcga
     3721 caggctgagc ggttcggtcg gcacgttact cacgtcatag tgcaggaagg caatcaagct
     3781 ttggatgcca atcaacacag gcagggccgc aagcatgacc gtgccgctgg tggcggggtg
     3841 gctgttgaag ttgccggaaa cccagtgcca ggtgccaaac gcagtaccgg cgagcagcaa
     3901 tagcacgcca ctcaggctgt acagcgtgcc tacgttgaag tcgcgcacca gatagttgta
     3961 cacatagcgg cgccacaagc gtgaaatgtg cttcttaagg aattcaggca gcacgcgccc
     4021 gaccttgagg ttggactcct cattcgcata gaccgagtcc atcggaatgt ccttcaccac
     4081 ggcgcgcaca gtgttcaggc gaaatagcat gtcggtttcg aagaaatagc gctgctccag
     4141 cttgtccagc ggcagctcgg ccaacacgct ggtacgcacg gccgtatagc cgttggtggg
     4201 gtccattacg ttccagtagc cacagctgag tttggtcaga aaggacagca tggcattccc
     4261 aaacagccgc accggcggca tgccctgtac cgactcgggc ctgaagaagc ggttgccctt
     4321 ggtgtagtcg gcccgcccct gcagcagcgg ccgcacgaag ctgggcagca atgctgggtc
     4381 catctgtcca tcaccgtcga tcttcaccac gatatccatg ccatcggcca tggcggcctg
     4441 gtaggcggtg acgatggcgc cgcctacgcc acggttctcg gggttgtaca gcacccgcac
     4501 gcgcgggtcg cggttgtgtt cctcaattaa gcggccgctg ccgtccggac aggcatcgtc
     4561 caccgcgtaa atggcctcga cttcagggcc gatggcttgg ataacttcca ggacatgccg
     4621 ggtgactttg tagcagggaa tggcgacggc gatgcgcatg acaaacagag ctcagctcgt
     4681 ggaggccagc gctacgccgg ccatgatcaa ggcgccgcca gccaaggtgc gccagtgcag
     4741 gggctcaccc agaaaggccc aagccagcgt tggcacgatc aggaaggcca ggcccatgaa
     4801 gggataggca aggtagagcg gggcatgacg aagaatccag acccatccca gcgtggtgat
     4861 gccatagagc accaaagccg ccatcagcca gccgttttgc agcagaccga tcagagacag
     4921 ggtggcgggt agggcagcgg acgccttctt gaacagcaac tggccgatgg agatgccgaa
     4981 cacgcaaagt agcgtgtaca ggccaagggg gattgacatg gaaggcaata gggccgagcg
     5041 aaaacccatg gattctaggc gggcacgcag tttggcagtc ttttgagccg gccataatcg
     5101 cgagcccgat cacgtcaatc aggtgcatag cactgattcg ccattcatga tgcaccgatt
     5161 gtttttggtg ttttttctat tgctgagtac cctgtcggct actgttcggg gtgcggaagt
     5221 gccggaggtg catggggcga ttgagtcctg gagctatgac ccatcaatgc gccaagtgag
     5281 ccttcgaggt tggagtttgg gagggggtga tggccaacag ccgcccgaac tcatcctgaa
     5341 tctggggggc aaatctatga atcgacaaga ctggagttga actggacacc acgagtcgat
     5401 attccatcag accatccggc gactggtggg caggcggggg ttggatttgc ttggaatgtg
     5461 cgcttgcgtg atgccttgcc agccggcatg cacacggtgc atctgacggc cgtgttcgca
     5521 aatggacagc gtgtcacgct caaaggtgcg accaccgatg tgccaacggt agtgattcag
     5581 gaaatcaagc gccgacactg gcgtgcgctg gccttggtgc tgctgagcgt gatggtcatt
     5641 gtctatgcgc tgcgtcgctg gggtagtgag gggttggcgt ggcagcagcg gtggctggat
     5701 ggcaatcgca tggcatgggg tattggtgcg gtgttcgtgt tgttggttgc caccggtgtg
     5761 acaggcagct ccatgggggt gttgttcaac agtccatatg gagaaagcgt tcttgaagtg
     5821 caaggggcct cgcgtacctt gcttggtgac gaaggcgttc gtggcgatga atggggcgtg
     5881 caattgccca acctgttggc acagctgcac cacgagcccc gtttcccggt gatcaatggg
     5941 ctactaggcg agggcggtca gaacatggga gtgatcggga tgaccggcgt tcctgtgcag
     6001 caatgggcgg cgctggcgcg gcctgcaacc tggggctatt tcgtactgcc gttgcgccaa
     6061 gccatggcgt ggcattggca gttgccgttt tggggttgtt tgctggcttt gtggtggatg
     6121 ctcaatgtgc tgcggccggc acagcgaggg ctgagcctgg ccttgtcact agcgttttgt
     6181 ctagcgccgt atgcggcagc gtggtctaac tggccgctgt acgccacctt gtttccggct
     6241 cttgcattcg tagtggcaac gtaccttctg cggtgtaccg ggttctggat gggtgcggtg
     6301 ctgggggctg ttttgggctg gttattggcg tgctggtgcc tggtacttta cccggcatgg
     6361 atcatcgttg tgggtagtgc gatggccttt ataggtctgg gctggtgggc cgataaccgt
     6421 gaacgcattc gctttggaag cgtgcaactg gtggcgctgg tcgtatctgc tgcgatcctg
     6481 ggggcactgc tgggcagttg gtggttggac accaaggatg caatcgccct tatgcgcgct
     6541 acggtgtatc ccggcggacg cggtgccatg ccgggggggt gatctgggat ggtggtggca
     6601 tttgcgtggc tatcacggca tggagtcgct tcgtactacg ggactggaca gcaacccatc
     6661 ggaagctag
//
LOCUS       DAOMTQ010000158         6628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2674_length_6628_cov_26.164156, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000158 DAOMTQ010000000
VERSION     DAOMTQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_6628_cov_26.164156"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1881
                     /locus_tag="PK518_13025"
     CDS             91..1881
                     /locus_tag="PK518_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRM49878.1"
                     /translation="MSYTAPVKDMLFAIEHLARIDHVAQIPGFEDAGLDTAAAVLEEC
                     AKFNEGVVAPLNVEGDRNPSSFKDGQVTTTPGFKDAYAQFTEAGWQGLQHPAAFGGQG
                     LPKTIGAACGEILHAANLSFALCPLLTDGAIEALLTAGSEELKAIYLEKLISGQWTGT
                     MNLTEPQAGSDLAQVRTKAEPQGDGTYKVFGTKIFITYGEHDMAENIVHLVLARVAGA
                     PEGVKGISLFVVPKFMVNSDGSLGARNDAHCVSIEHKLGIKASPTAVLQFGDHGGAIG
                     YLVGEENRGLEYMFIMMNAARYGVGVQGIAVAERAYQQAVQYARERVQSRPVDGSLPA
                     AGPIIHHPDVRRMLMTMRAYTEGCRAMASVAAAAFDAAHHHPDPQVARDNAAFYEFLV
                     PLVKGYSTEMSQEVTSLGVQVHGGMGFIEETGAAQHYRDSKILTIYEGTTAIQANDLV
                     GRKTARDGGQLAKAVAAQIEQTEAELQASGTADAQAVARRLAAARQAFLDAVDFMAAN
                     TKANPNAAFAGSVPYLMLTGNLVAGWQMGRALLVAQQLSSKGQDQAFMQAKIATARFY
                     AEHILVKAPAQRDAIVEGAASVTALALDAF"
     gene            1961..2917
                     /locus_tag="PK518_13030"
     CDS             1961..2917
                     /locus_tag="PK518_13030"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRM49879.1"
                     /translation="MSSLPPALQKLSLPVIGAPLFIISNPQLVIAQCQAGIVGSMPAL
                     NARPAAQLEDWLAEITETLAAWDRAHPEHPAAPFAINQIVHKSNDRLEHDMQVCAKYK
                     VPIVITSLGAREDVNQAVHGWGGIVLHDIINNKFARKAIEKGADGLIAVAAGAGGHAG
                     VKSPFALIQEIRQWFDGPIALSGAIASGGAILAAQAMGADFAYIGSAFIATKEARAHD
                     AYKQAIVDGSSDDIVYSNLFTGVHGNYLAASIRAAGLDPENLPESDPSKMNFGGDAKK
                     AWKDIWGCGQGIGSITEVVPAAELVARLAREYQTARARLTLA"
     gene            complement(2930..3763)
                     /locus_tag="PK518_13035"
     CDS             complement(2930..3763)
                     /locus_tag="PK518_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRM49880.1"
                     /translation="MAPAALRFLNHLARRVRAAVAPVPDIGAELWLQTLGRHPFLAAL
                     PLQDQAKLRALSALFLQRKEFSGAHGLVVTDAMAVAIAAQACLPLLHWGAPDKALAWY
                     DDFVGIVVHPAEALARRKAVDEAGVVHHYHEVLLGEAMEHGPVMLSWPAVDGAGRHGA
                     TSVVIHEFMHKIDMRNGPADGCPPLPAGFLGQAGARNAHAAWQAAWQPAYEQFREQVI
                     IAERFGGDWPWLDAYGATAPAEFFAVACEAYFVDRPRFAQEFPTLAPMLQAFFQPALT
                     G"
     gene            complement(3768..4550)
                     /locus_tag="PK518_13040"
     CDS             complement(3768..4550)
                     /locus_tag="PK518_13040"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRM49881.1"
                     /translation="MQQAVPPMAELVAPAGWRTVDFLADLHLEPGQPATLQALRQYLE
                     STPADAVFLLGDLFEVWVGDDAIDEPDSFEGACSALLAGAARQRPMYFMHGNRDFLVG
                     AEFTRRTGVALLADPTVLQFLDQRWLLSHGDALCLNDVEYQRFRAVARNSAWQAQLLA
                     RPLHERRAQGRSARSESEARKQAGTAVYADVDEPTALAWLQAARAQALIHGHTHMPAD
                     HVLAQGLARHVLTDWDLAADPPRAGLLRLTAQGLQRLPIAPM"
     gene            complement(4559..5086)
                     /locus_tag="PK518_13045"
     CDS             complement(4559..5086)
                     /locus_tag="PK518_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM49882.1"
                     /translation="MSNPQVELHVTINVAETATQGVITLELDAVNAPKCTANFLAYVN
                     KGHYDGTIFHRVIKGFMIQGGGFTADMKQKGTDAPIENEAANGLKNDKYTIAMARTGE
                     PHSATAQFFINTVDNGFLNHTSPTPQGWGYAVFGKVVKGEDVVESIKKVRTTRKGFHD
                     DVPFDAVVIDKAVAL"
     gene            complement(5104..5679)
                     /locus_tag="PK518_13050"
     CDS             complement(5104..5679)
                     /locus_tag="PK518_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM49883.1"
                     /translation="MISRRNTAVAVAGIALAAMFSTANAQQAPKVKLATSLGDIVVQL
                     DPAKAPKTVENFLAYVQDKHYDGTVFHRVIDGFMIQGGGFTPDLVQKSTRAPIALEAN
                     NGLKNDKYTIAMARTSIPDSATAQFFINVNNNAMLNAPSPDGHGYAVFGKVVEGMAVV
                     DKIKGVATGNKGPHQNVPNTPVLIQSATLVK"
     gene            complement(5676..>6628)
                     /locus_tag="PK518_13055"
     CDS             complement(5676..>6628)
                     /locus_tag="PK518_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM49884.1"
                     /translation="APQPSAAASALPASATDTLSSLRSELRLRLDSTKAPPPQGSIPA
                     QFLELSARTRHAIAMDASRSRLYLFENTDKGLVLVADYYASVGKLGTEKVVEGDQRTP
                     LGVYYITSRLDPATLKDFYGAGALPINYPNPLDQSRGKTGSGIWLHGTPPDQFSRAPQ
                     ATDGCLALANPDLERILKTVEPRTTPVVIARQLQWVKPHALQAERQSFHAVLEAWRTA
                     RSHGDMQRLLTFYAPEFQGLRNLDLEQWTQTLQAEHRTLRGRAVLLKDKSMLRWTDSS
                     DTIVVTFGELAEGARTGTTRRQYWTRKGAHWQIFFEGVIG"
BASE COUNT         1075 a         2158 c         2269 g         1126 t
ORIGIN      
        1 acgcgctctg agcgcgcgcc accgtaccgg ggcatcgcgt gcgatgccct ttttttcgcc
       61 acccatctcc atccaaccat atccattccc atgagctata ccgcccccgt caaggacatg
      121 ttgtttgcca tcgagcacct ggcgcgcatc gaccacgtcg cgcagattcc cggctttgag
      181 gatgcgggcc tggacacggc cgccgccgtg ttggaggaat gcgccaagtt caacgagggg
      241 gtggtggcgc cgctgaacgt ggagggcgac cgcaacccgt cctcgttcaa ggacggtcag
      301 gtcaccacca cgcccggttt caaggacgcg tacgcccagt tcaccgaggc cggctggcag
      361 gggctgcagc acccggcagc ctttggtggc cagggcctgc ccaagaccat tggagcggcc
      421 tgcggcgaaa tcctgcacgc cgccaacctg agctttgcgc tgtgcccgct gctcactgat
      481 ggcgccatcg aggcgctgct caccgctggc agcgaggaac tcaaggccat ttacctggag
      541 aagctgattt ccggccagtg gaccggcacc atgaacctga ccgagcccca ggccggctcc
      601 gatctggccc aggtgcgtac caaggccgag ccgcagggcg acggcaccta caaggttttc
      661 ggcaccaaga tctttatcac ctatggtgag cacgacatgg ccgagaacat cgtgcacctg
      721 gtgctggcgc gcgtggccgg ggcgcccgag ggcgtcaagg gcatcagtct gttcgtggtg
      781 ccgaagttca tggtcaatag cgatggcagc ctgggcgcgc gcaacgatgc gcattgcgtc
      841 tccatcgagc acaagctggg gatcaaggcc agccccacgg ccgtgctgca gtttggtgac
      901 cacggcggcg ccatcggtta tttggtgggc gaggagaacc gcggcctgga gtacatgttc
      961 atcatgatga acgccgcccg ctacggcgtg ggcgtacagg gcattgcggt ggccgagcgc
     1021 gcctaccagc aggccgtgca gtatgcgcgc gagcgcgtgc aaagccgccc cgtcgatggc
     1081 agcctgccgg ccgccggccc catcatccac caccccgatg tgcgccgcat gctgatgacc
     1141 atgcgtgcct acaccgaggg ctgccgcgcc atggccagcg tggccgcggc cgccttcgat
     1201 gcggcgcatc accatcctga cccgcaggtg gccagggata acgcggcctt ctatgagttc
     1261 ctggtgccgc tggtcaaggg ctacagcacc gagatgagcc aggaggtcac cagcctgggt
     1321 gtgcaggtgc atggcggcat gggcttcatc gaggaaaccg gggccgccca gcattaccgc
     1381 gacagcaaga tcctgaccat ctacgagggc acgaccgcca tccaggccaa tgacctggtg
     1441 gggcgcaaga cggcgcgcga cggcggtcag ttggcgaagg cagtcgccgc gcagatcgag
     1501 cagaccgagg ccgagctgca ggccagcggc actgccgacg cccaggccgt ggcccggcgg
     1561 ctggcagccg cgcgccaggc ctttctggat gccgtggact tcatggccgc gaataccaag
     1621 gccaacccga acgccgcctt tgccggcagc gtgccctacc tgatgttgac cggcaacctg
     1681 gtggccggct ggcaaatggg tcgcgcgctg ctggtggcgc agcagctgtc gtccaaggga
     1741 caggatcagg ccttcatgca ggccaagatc gccacggcgc gcttctatgc cgagcacatt
     1801 ctggtgaagg ccccggcgca gcgcgacgcc atcgtcgagg gcgcggccag cgtcacggcg
     1861 ctggcgctcg atgcgttctg acgattgata ttgttttgat agctgcgtgc gcttgatgga
     1921 taagcgctgc agccctgttt attgccaagg agactgccct atgtccagct tgccgccggc
     1981 cctgcaaaag ctctcgctgc ccgtgatcgg cgcgccactg ttcatcatca gcaaccccca
     2041 gctggtgata gcgcagtgcc aggctggcat cgtcggctcc atgcccgcgc tcaacgcccg
     2101 cccggccgcg cagctggagg actggctggc cgagatcacc gagaccctgg ccgcctggga
     2161 cagggcgcat ccggagcatc cggcggcgcc gttcgccatc aaccagatcg tgcacaagag
     2221 caatgaccgg ctggagcacg acatgcaggt ctgcgccaaa tacaaggtgc ccatcgtcat
     2281 cacgtccctg ggtgcgcgcg aggacgtgaa ccaggccgtg catggctggg gcggcatcgt
     2341 gctgcacgac atcatcaaca acaaatttgc gcgcaaggcg atcgagaagg gcgccgacgg
     2401 cctgatcgcc gtggcggccg gcgccggcgg tcatgcgggc gtgaagagtc cgtttgcgct
     2461 catccaggag atccgccagt ggttcgacgg cccgatcgcg ctgtcgggcg ccattgcctc
     2521 gggcggcgcc attctggccg cgcaagccat gggcgcggac tttgcctata tcggctcggc
     2581 cttcatcgcc acgaaggagg cgcgcgccca cgatgcttac aaacaggcca tcgtcgatgg
     2641 ctcgtcggac gacatcgtct acagcaacct gttcaccggc gtgcatggca actacctggc
     2701 ggcctcgatc cgcgccgccg gcctggaccc ggagaacctg cccgagtccg acccgagcaa
     2761 gatgaacttt ggcggcgatg ccaagaaggc ctggaaggac atctggggct gtggtcaggg
     2821 cattggcagc attaccgagg tggtgccggc ggcagagctc gtcgcacgcc tggcgcgcga
     2881 ataccagacg gcacgcgcgc gtctgacgct ggcttgatgc gttacgccgt cagcccgtga
     2941 gggcgggctg gaagaaggcc tgcagcattg gcgccagcgt cggaaactcc tgcgcaaagc
     3001 gcggcctgtc gacaaagtag gcctcgcagg ccacggcaaa aaattctgcc ggcgccgtgg
     3061 cgccataggc gtccagccag ggccagtcgc cgccaaagcg ctcggcgatg atcacctgct
     3121 ctctgaactg ctcataggcc ggctgccagg ccgcttgcca ggcggcatgc gcgttgcgcg
     3181 ccccggcctg gcccaaaaag cccgcgggca gcggcgggca gccgtccgcc gggccgtttc
     3241 tcatgtctat cttgtgcatg aactcgtgga tcaccacgct ggtcgcgcca tggcggccgg
     3301 cgccgtccac ggccggccag ctcaacatga cggggccatg ctccatggcc tcgcccagca
     3361 gcacctcgtg gtagtggtgc accacgccgg cctcgtccac ggccttgcgc cgcgccaggg
     3421 cctcggccgg gtgcacgacg atgccgacaa agtcgtcata ccaggccagc gccttgtcgg
     3481 gcgcgcccca gtgcagcagc ggcaggcagg cctgggcggc gatggcgacg gccatggcgt
     3541 cggtcaccac caggccatgg gcgccggaaa actccttgcg ctgcaggaag agggcactga
     3601 gcgcgcgcag cttggcctgg tcctgcagcg gcagggcggc caggaagggg tggcggccga
     3661 gcgtctgcag ccacagttcg gcgccaatgt cgggaaccgg cgccacggcg gcgcgtacac
     3721 gccgggcgag gtggttcaga aaacgcaatg cagcgggagc catgccgcta catgggggcg
     3781 atgggcaggc gttgcaatcc ttgggccgtc aggcgcagca atccggcgcg cggcgggtcg
     3841 gcggccagat cccagtcggt gagcacatgg cgcgccaggc cctgggccag cacatggtcg
     3901 gccggcatat gggtgtggcc gtggatcagc gcctgggccc gcgcggcctg cagccaggcc
     3961 agggcggtgg gctcgtccac atcggcatag acggccgtgc cggcctgctt gcgcgcctcg
     4021 ctctcgctgc gggcgctgcg gccctgggca cggcgctcgt gcagcgggcg cgccagcagc
     4081 tgggcctgcc aggcgctgtt gcgcgcgacg gcgcgaaagc gctggtactc gacatcgttc
     4141 aggcacagcg cgtcgccatg cgacagcagc cagcgctgat ccagaaattg cagcaccgtg
     4201 gggtcggcca acagggcaac gccggtgcgg cgcgtgaact ctgcgcccac cagaaagtcg
     4261 cggttgccat gcatgaagta catgggccgc tggcgcgcgg cgccggccag cagggcgctg
     4321 catgcaccct cgaagctgtc cggctcatca atggcgtcgt cgcccaccca gacctcgaac
     4381 aggtcgccga gcaggaacac tgcatcggcg ggcgtgctct ccagatactg acgcaatgcc
     4441 tgcagcgtgg ccggctggcc cggctccagg tgcaggtcgg ccaggaaatc gaccgtgcgc
     4501 cagcccgctg gcgcgaccag ctcggccatc ggcggcacgg cctgctgcat ggcctggctt
     4561 acagcgccac ggccttgtcg atcaccacgg cgtcgaacgg cacgtcgtcg tggaaaccct
     4621 tgcgcgtggt gcgcaccttc ttgatgctct ccaccacgtc ctcgcccttg accaccttgc
     4681 cgaacacggc atagccccag ccctgggggg tgggcgaggt gtggttcaaa aagccgttgt
     4741 ccaccgtgtt gatgaagaac tgggccgtcg cgctgtgcgg ctcgccggtg cgcgccatgg
     4801 cgatggtgta cttgtcgttc ttcaggccgt tggcggcctc gttctcgatg ggcgcgtccg
     4861 tgcccttttg cttcatgtcg gcggtaaagc cgccgccctg gatcatgaag cccttgatca
     4921 cgcggtggaa gatggtgccg tcgtaatggc ccttgttcac gtaggccagg aagttggcgg
     4981 tgcacttggg cgcgttcacg gcgtccagct ccagcgtgat cacgccctgg gtggcggtct
     5041 cggcgacgtt gatggtgacg tgcagttcga cttgcgggtt gctcatggcg cttttccttt
     5101 cggttatttg accagggttg cggattggat cagcacgggc gtgttgggca cgttctggtg
     5161 cgggcccttg ttgccggtgg cgacgccctt gatcttgtcg accaccgcca tgccctcgac
     5221 gaccttgccg aacacggcat agccatggcc gtccgggctg ggggcattga gcatggcgtt
     5281 gttgttcacg ttgatgaaga actgcgccgt ggcggaatcc gggatggagg tgcgtgccat
     5341 ggcgatggtg tacttgtcgt tcttcagacc gttgttggcc tccagtgcga tcggcgcgcg
     5401 cgtgctcttc tgcaccaggt cgggcgtgaa accgccgccc tggatcatga agccgtcgat
     5461 gacgcggtga aacaccgtgc cgtcgtaatg tttgtcctgg acataggcga ggaagttctc
     5521 caccgtcttg ggcgccttgg ccggatcgag ctgcaccacg atgtcgccca ggctggtggc
     5581 cagcttgacc ttgggggcct gctgtgcatt tgctgtagaa aacatagctg ccagcgcaat
     5641 accggcaacg gctacagccg tatttcttct ggaaatcatc caatcactcc ttcaaaaaat
     5701 atctgccagt gcgcgccctt gcgcgtccag tactggcgcc tggtggtgcc ggtgcgcgcg
     5761 ccttcggcca gttcgccgaa agtgaccact atggtgtcgc tgctgtcggt ccagcgcagc
     5821 atgctcttgt ccttgagcaa taccgcgcga ccgcgcaggg tgcggtgctc ggcctgcagc
     5881 gtctgcgtcc actgctccag atcgaggttg cgcaggccct ggaactcggg tgcgtagaag
     5941 gtcagcaggc gctgcatgtc gccatgcgac ctggccgtgc gccaggcctc gagcacggca
     6001 tggaaggact ggcgctcggc ctgcagtgcg tggggcttga cccactgcag ctggcgcgcg
     6061 atgaccaccg gggtggtgcg cggctccacg gttttcagga tgcgctccag atccgggttg
     6121 gccagggcca ggcagccgtc ggtggcctgc ggcgcgcgcg agaactgatc gggtggcgtg
     6181 ccgtgcagcc agatgccgct accggtcttg ccccggcttt ggtccagcgg gttggggtag
     6241 ttgatgggca gggcgccggc gccgtaaaaa tccttcagcg tggccgggtc gagccggctg
     6301 gtgatgtagt acacgcccag aggtgtgcgc tggtcgccct cgacgacttt ctcggtgccg
     6361 agcttgccca ccgaggcgta gtagtcggcc accagcacca ggcccttgtc ggtgttctcg
     6421 aacaggtaca gccgcgagcg cgaggcatcc atggcaatcg catggcgcgt gcgtgccgac
     6481 agctccagaa actgcgccgg aatgctgccc tgtgggggcg gtgccttggt cgagtccagg
     6541 cgcaggcgca gttcactgcg caggctggac agcgtgtccg ttgccgacgc cggcaaggcc
     6601 gacgcggcgg cgctgggctg gggggcgg
//
LOCUS       DAOMTQ010000159         6533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2731_length_6533_cov_31.934393, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000159 DAOMTQ010000000
VERSION     DAOMTQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2731_length_6533_cov_31.934393"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1050)
                     /locus_tag="PK518_13060"
     CDS             complement(1..1050)
                     /locus_tag="PK518_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM49885.1"
                     /translation="MTGRSSILAALAAALLFGASTPFAKQMVGDVSPIMLAGLLYLGS
                     GIGLWTVRLIRDRGFAALGLPAREWPWLLGAIASGGVLGPLLLMIGLAHTTAADASLL
                     LNMEAVLTAVLAWVVFRENADRRIVLGIAFIVAGGLLLAWPQHDGMGQSSTLGSLAVA
                     GACLCWAVDNNLTRKVSASDAVFIAGTKGLVAGATNLALALALGATLPAWPRVAQAMS
                     VGLAGYGVSLVLFVLALRGLGSARTGAYFSTAPFIGAAIAIVALGEPTSGVFWLAAAL
                     MGVGVWLHLTERHEHAHTHEPLAHAHRHVHDEHHRHTHDFPWDGVEPHAHPHRHEPLS
                     HRHPHFPDVHHQHSH"
     gene            1913..2227
                     /locus_tag="PK518_13065"
     CDS             1913..2227
                     /locus_tag="PK518_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HRM49886.1"
                     /translation="MATIGTFTADKDGFTGTLRTLTLNIKAKLVPNDKGDNEHAPDFR
                     VQAAGFDIGAAWKKVSKAERPYVSVTLDDPSFPATVYARLIEDEDGTHTLIWSRSKPQ
                     AA"
     gene            complement(2588..2881)
                     /locus_tag="PK518_13070"
     CDS             complement(2588..2881)
                     /locus_tag="PK518_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM49887.1"
                     /translation="MAAKHTLSEALKTIRKARGLSQEAFSDVSSRTYMSSLERDLKSP
                     TLNKLAELCEVMEVHPLTLLTLAYAGDDAQRAEQLLAQVRQELEAMLKKDDAQ"
     gene            3106..3453
                     /locus_tag="PK518_13075"
     CDS             3106..3453
                     /locus_tag="PK518_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HRM49888.1"
                     /translation="MNRLITEDERRQLLAHGQSRAAGQAIDPLPVVRLFTPDAHATWL
                     LAALDPADGDTAHGLIDLGISMPELGTVKLSDLAAIVGPRQQPVMRDRYFQAVRPLSE
                     YLRLAQKNGSVVD"
     gene            3794..4543
                     /locus_tag="PK518_13080"
     CDS             3794..4543
                     /locus_tag="PK518_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HRM49889.1"
                     /translation="MGNPHHLAHWYPTAAYLYILGLDALALAWEYLRRHPDYRLDWLR
                     RARRPDAAQRWGLRLLEDPALDAREAYPAWVPGHDAVVQLYPDADPPQDATTFAFWRI
                     PGHKQLLHDGKGLMLIARSPGHWQRFVLAPGLEDGMAVAHAYRGRGVTHAPDTPAPMA
                     RPRPPPAALLELHTLQALDATLAGASSRDVAEGLFGADAVVDWYSDGGLRSKVRRLAR
                     RGDALMRGGYRRLAQLSPLEKGRFEGDAKRP"
     gene            4627..4908
                     /locus_tag="PK518_13085"
     CDS             4627..4908
                     /locus_tag="PK518_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM49890.1"
                     /translation="MRPAPLRPAATVSTAAAQPQRYLTNDEAAEYLRLSPRTLEKQRV
                     IGGGPRFRKFGRRVMYAVADLDAWAADRSFETTSDPEYAEHHSADSRAR"
     gene            4935..5783
                     /locus_tag="PK518_13090"
     CDS             4935..5783
                     /locus_tag="PK518_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HRM49891.1"
                     /translation="MSSPALPMRQRPLQEREQLDLFRALPGDMAPRDSQDLMAYPFFS
                     LGKSKRVKPIDFRAGNVTICVEGTAEHGIATIWDADILIWAASQIMEAKDAGLQPSRL
                     MRATPYEILRFIGRGKSLRDYQRLKAALDRLQSTTVATSIRETTGRRLHRFSWINEWK
                     ELADAKGAPLGIELILPDWFYAGVLDAALVLTIDPAYFRMTGGIERWLYRLVRKHGGR
                     QPGGWQFDFRHLHRKSGSTAKPYDFACDLRALVARQSLPGYVLGIERLDGAELLTFRP
                     VPYTAR"
     gene            6052..>6533
                     /gene="parA"
                     /locus_tag="PK518_13095"
     CDS             6052..>6533
                     /gene="parA"
                     /locus_tag="PK518_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HRM49892.1"
                     /translation="MIVALLNQKGGVGKTTLATHIAGELAMRGQHVVLLDADPQGSSL
                     DWTQRRSQQGLPRLFSAVGLARETLHQEAPELARRADHVVIDGPPRIAALARSALLAA
                     ERVLIPVQPSPYDLWASAEMVALIREAQVFRPALRAAFVINRRVSTTVIGREARQALA
                     "
BASE COUNT         1083 a         2209 c         2111 g         1130 t
ORIGIN      
        1 ctaatggctg tgttggtgat gcacgtccgg gaagtgcgga tggcggtgcg agaggggctc
       61 atgccgatgc gggtgggcgt gcggctcgac gccatcccag gggaagtcat gtgtgtggcg
      121 gtggtgttcg tcatgcacat gccggtgggc atgtgccagg ggctcatgcg tgtgcgcgtg
      181 ttcgtgccgc tcggtcaggt gcagccagac gcccaccccc atcagcgccg ccgccagcca
      241 gaacacaccc gaggtcggct cacccagggc cacgatggcg atggcggctc cgatgaaggg
      301 tgctgtcgag aagtacgccc ccgtgcgggc cgagccgagg ccgcgcaagg ccagcacgaa
      361 cagcaccagg ctgacgccgt agccggccag tccgacgctc atggcctgtg ccacgcgcgg
      421 ccaggccggc agcgttgcgc cgagcgccag tgccagcgcg aggttcgtgg cgcccgccac
      481 cagtcctttg gtgcccgcga tgaaaaccgc atcagaggcc gacaccttgc gcgtcaagtt
      541 gttgtccacg gcccagcaca ggcaggcgcc ggccacggcc aagctgccca gcgtgctgct
      601 ctgtcccatg ccatcgtgtt gcggccaggc cagcagcagg ccacccgcca cgatgaaggc
      661 tatgcccagc acgatgcgtc ggtcggcgtt ttcccggaag acgacccatg ccagcacggc
      721 cgtcagcacc gcctccatat tcagcagcag cgaggcatcg gcggccgtgg tgtgcgccag
      781 cccgatcatc agcagcaagg ggcccagcac gccgccactg gcgatggcgc caagcagcca
      841 gggccattcc cgcgcgggca ggccgagcgc ggcaaagccg cgatctctga tgaggcgaac
      901 ggtccacagg ccgatgccgc tgcccaggta cagcaggccg gccagcatga tgggcgacac
      961 gtcgccgacc atctgcttgg cgaagggcgt gctagcaccg aacagcagtg cggcggccag
     1021 ggcggcgagg atggaggagc ggcctgtcat ggagggtgaa cttacgaatc gaggggcaaa
     1081 tatcacctta caccgattcc ataacgcctc gatgatgcgg ggctggtttg agcgttgatg
     1141 cagcagggcg tatcggcgca atgcgccgcc gggctattgg gctgcgcccc gtgccgactt
     1201 tgtcgtcacg tccattcggc ggctatccct cacgcacctt cgcgcctgcg gcgcagcgct
     1261 tacctccggg ggaaagccct gcgggctatc cccgccgcgc aggcttggcg cccctcgata
     1321 ccaccgaacc ggcagcgccg gatcggccgg gccagcgccc cgtcgcatgg gcagtgcccg
     1381 gtgggtacgc cggggcttgc tgcggcaggt tgtgcgaatg cgtgccgtcc acgacgctgg
     1441 gggctcattg cccgtgttgt cacgaaggac ggttcacgga tggtcgctcg gccttgctgg
     1501 ggactccaca ccgtgcgttc agcatgaccg cctgcatggg atgaggcgca ttcccgctgg
     1561 tcgttgggga atggccttgt cgagtcaggg ggcgtgccgc tgaagccttc gtgcggggat
     1621 gccggggtgg ccatggctcc tttcggatgg ggggtggttc ggcccaaggc gtccttgtgt
     1681 tcttgtgaat cctggcgggg gcgcggctgc gcctcgggct tcatggctgc aaccgtccgg
     1741 cgaaaacaat ttccccgccg ctgtgcggct cctcgcagga caaattgttt ttgccccccg
     1801 gttctccact gcgttgcgac cgcaagcggt gcagcccgcc cgtccaccgc cggtcggatc
     1861 acaacaagga cgcgataggc gcgaaccttg ttcaaccgta aggagattca tcatggcaac
     1921 catcggcacc ttcaccgcgg acaaagacgg cttcaccggc acgctgcgca ccctgaccct
     1981 caacatcaag gccaagctgg ttcccaacga caagggggac aacgagcacg ccccggactt
     2041 ccgggtacag gcggcaggct tcgacatcgg cgcggcgtgg aaaaaggtca gcaaggccga
     2101 acggccctac gtgtccgtga ccctcgacga tccttcgttc ccggccacgg tctatgcccg
     2161 gctgattgag gacgaagacg gcacgcatac gctgatctgg tcgcgcagca agccccaggc
     2221 ggcctgacgg ccgcccagcg ccccgcccga gcggcggggc gctgggctgc tttcgcagca
     2281 cggcatggag gcaacatcgt cactggagcg cgacccgtgt cggcctgtta cagcgttgag
     2341 accgtggttg cacggacagc atggcgtgcc ggtgctttgc accgccgggc tttcgggctg
     2401 ggccccgtgc cggctgcgcc gtcacggcca ttcggcttca atccctcacg ccttcgcgcc
     2461 tgcggcgctg tgcgctttgc ttgcctccag gggaaagcct tgcggctatc cccgccgcgc
     2521 gacgcttggc gcccctgaaa actccgaacc ggctgtgccg gatcggccag gccaacgcca
     2581 atgcaggcta ctgcgcatcg tctttcttca gcatggcctc cagctcctgc cgcacttgtg
     2641 ccagaagctg ctcggcccgc tgcgcgtcgt cgccggcata ggccagcgtc aacagcgtga
     2701 gcgggtgaac ctccatgacc tcgcacagct cggcgagctt gttcaaggtg ggacttttca
     2761 ggtcgcgctc cagcgaactc atgtaggtac ggctggacac atccgagaag gcttcctggc
     2821 tcagtccacg cgccttcctg atggtcttca acgcctccga caatgtgtgt ttcgctgcca
     2881 accttggatc tccccgaaat ccaagatgac agttgattgc gccttataga gctacaatct
     2941 atagtgttca ttactgaccg attatcgctc ttgtgctttt atggcaatcc gtattcacgg
     3001 cttccgacgc aagcgcaaaa ccgcatccgt ggttttcctc aaacccgtcg atccggttct
     3061 acgctttcac gctttggcgg atttgtgcag caagaaggga ttgccatgaa ccgactcatc
     3121 accgaagacg agcgcaggca gttgctggcc cacggccaat cccgggccgc tggccaagcc
     3181 atcgacccgc tgcccgtggt gcggctgttc acgccggatg cccatgccac ctggttgctg
     3241 gctgcacttg acccggccga tggcgatacg gcccacggcc tgatcgacct cgggatcagc
     3301 atgcccgagc tgggaacggt gaagctctcc gacctcgctg ccatcgttgg gccccgccag
     3361 cagccggtga tgcgggatcg gtatttccag gcggtgcggc ctctgtcgga atacctgcgg
     3421 ctggcgcaga agaacggttc cgtcgtcgat tgaaacgttc gcgcgtcagc caagtggggc
     3481 gcattcagac tatttcagtc tggctccaga ctcaccacgt ctcaatcagc actcttgcgc
     3541 gcaggccctg tacttgtgct ggaaacagtc acgatctttc atgcagcttg cggcacggta
     3601 tctgatgccg cgcgatgttt tggaattgcg cggtcgtcgt attcggacga atttcgcaat
     3661 acaccggagc ccatcggtct tgacacccaa tgttacagcc caacccgatt gtgatggcag
     3721 ctgatcgtct gcgatagccc cgtgttgaac gcccgtgacg acgttcctga tcttcgacag
     3781 gagcttggcg atcatgggca accctcacca tctcgcgcac tggtatccga ccgctgccta
     3841 cctctacatc ctgggcctgg atgcactcgc gctggcctgg gagtacctgc gcaggcatcc
     3901 cgattaccgg ctcgactggc tgcgccgtgc gcgccgcccc gatgctgcgc agcgctgggg
     3961 cctgcgcctg ctggaagacc cggccctgga tgcgcgcgag gcgtatccgg cctgggtacc
     4021 cggccacgat gccgtggtgc agctctatcc ggacgctgat ccgccgcagg atgccaccac
     4081 cttcgcgttc tggcgcatcc ccggccacaa gcaactgctg cacgacggca aggggctgat
     4141 gctgatcgcg cgaagccccg gccattggca gcgcttcgta ctggcgcccg gcctggaaga
     4201 cggcatggcc gtggcccatg cctatcgcgg tcgcggcgtc acgcatgcgc ccgacacgcc
     4261 ggcgccaatg gcccggccca ggccgccccc cgctgcgctg ctggaactgc acaccttgca
     4321 ggcgctggac gcgaccctgg cgggcgcgtc ctcacgcgac gtcgccgaag ggctgttcgg
     4381 cgccgatgcc gtggtcgatt ggtacagcga cggcggcctg cgctcgaagg tgcgccgcct
     4441 ggcacggcgt ggcgatgcgc tgatgcgcgg cggctaccgc cgcctagcac aactgtcccc
     4501 gcttgagaag ggtcgttttg aaggcgacgc aaaacggccc tgagcaaaag ggccgcattt
     4561 tctgagactg cctccatccg gttgcgctgt gtggccgggc gttttcaacc gatggaggtt
     4621 cacaccatgc gtcccgctcc cttgcggcct gccgccactg tctcgaccgc tgccgcgcag
     4681 ccccaacgct atctcaccaa cgacgaagcc gccgagtacc tgcgcctgtc gccgcgcacg
     4741 ctggaaaagc agcgcgtgat cggcggcggc ccgcgctttc gcaagttcgg ccggcgcgtc
     4801 atgtacgccg tggccgacct cgatgcctgg gccgccgacc gcagtttcga gacgacttcc
     4861 gatcccgaat acgccgagca ccactcggcc gacagccgtg cgcgctgatc ggcggcgcgc
     4921 ggcaggccat cgccatgtcc agcccggcgc tgcccatgcg gcagcggcca ttgcaggagc
     4981 gcgaacagct cgacctgttc cgcgccttgc ccggcgacat ggcgccgcgc gacagccagg
     5041 acttgatggc ctatccgttc ttctcgctcg gcaagtccaa gcgggtcaag cccatcgact
     5101 ttcgcgcggg caacgtgacg atctgcgtgg aaggcacggc cgagcacggc atcgccacta
     5161 tttgggatgc cgacatcctg atctgggccg ccagccagat catggaagcc aaggacgcgg
     5221 gcctgcagcc gtcgcggctg atgcgcgcca cgccctacga gatcctgcgc ttcatcgggc
     5281 gcggcaagtc gctgcgcgac taccagcgcc tcaaggccgc gctggatcgc ctgcaatcga
     5341 ccacggtggc cacgtccatc cgcgagacga ccgggcggcg cctgcaccgt ttctcgtgga
     5401 tcaacgaatg gaaggaactg gccgatgcca agggtgcgcc cttgggcatc gagctgatcc
     5461 tgccggactg gttctacgcg ggcgtgctcg atgccgcctt ggtgctgacc atcgacccag
     5521 cgtatttccg gatgacgggc ggcatcgagc ggtggctgta ccgcctggtg cgcaagcacg
     5581 gcggacgcca gcccggcggc tggcaattcg acttccggca cctgcaccgc aaatcgggca
     5641 gcacggccaa gccctacgat ttcgcctgcg acctgcgcgc cttggtggcg cggcagtcgc
     5701 tgccgggcta cgtcctgggc atcgagcggc tggatggcgc cgagctgctg acgttccggc
     5761 ccgtgccgta cacggcacgg tgataactgc ggtaaagcct gtggacgggc tcgtgctatc
     5821 aggcgtgcca ggtatcgtgc tatcaggcgt gggactatcg tgctatcagg cgtgtccatc
     5881 gcccgcaaag ccaatgctgg cgcgggtttc ggcctccctt aacttcccta acttaaaaac
     5941 tctaactggt agtagaagcg ccgcgcctcg gtggacaacc accaaaccgg cgaacgaagg
     6001 gctcgaaaac cagcagcaac agcccggctt tccaacacgg agggccaggc catgatcgtc
     6061 gctttgctca accagaaagg cggcgtgggc aagaccacgc tcgccaccca catcgccggc
     6121 gaactggcga tgcgcggcca gcacgtcgtg ctgctggatg ccgacccgca gggttcatcg
     6181 ctggattgga cgcaacgcag gagccagcaa ggcttgccaa ggctgttcag cgccgtgggc
     6241 ctggcacgcg aaacgctgca tcaggaagca ccagaactcg ccaggcgggc cgatcacgtt
     6301 gttatcgacg ggccgccgag gatcgccgcc ttggcgcgct ccgcgctgct ggcggccgag
     6361 cgggtgctga tcccggtgca gcccagcccc tacgacctgt gggccagcgc cgagatggtg
     6421 gcactgatcc gcgaggcgca ggtgtttcgg cctgcgctgc gcgcggcctt cgtcatcaat
     6481 cgccgcgtca gtaccaccgt gatcgggcgc gaagcgcgcc aggcgctcgc cga
//
LOCUS       DAOMTQ010000160         6527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2735_length_6527_cov_17.929234, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000160 DAOMTQ010000000
VERSION     DAOMTQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_6527_cov_17.929234"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..537
                     /locus_tag="PK518_13100"
     CDS             70..537
                     /locus_tag="PK518_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRM49893.1"
                     /translation="MSTAENDLYQEVVLEHKRAPRNFGQLAHPTHQAQGTNPSCGDRV
                     SVQLQMDGQRVADIRFTGQGCAICMASTSMMTEAVKGRDLAVAQELQQHFRAVLTGEE
                     DPEDAPLGKLVSLAGVRQYPSRIKCALLGWHALMHAIADNDGQAVSTEEEGQP"
     gene            534..1088
                     /gene="sufT"
                     /locus_tag="PK518_13105"
     CDS             534..1088
                     /gene="sufT"
                     /locus_tag="PK518_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HRM49894.1"
                     /translation="MMNRRHREDVLVRRNVMVETIPSGQPVELEQGMLAQITQALGSS
                     FTLLVEGQLMRLKGLDADAIGKTPPETIAVPEDLHLDDLEPLVWETLRTCYDPEIPVN
                     IVDLGLVYRLEFQPSDEADKVRAVIDMTLTAPGCGMGESIANEVCDKVLALPRVADIT
                     VNLVFDPPWDRSMMSEEAMLALGL"
     gene            complement(1250..2491)
                     /locus_tag="PK518_13110"
     CDS             complement(1250..2491)
                     /locus_tag="PK518_13110"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM49895.1"
                     /translation="MSTPFFDAVIIGAGAAGLFCAAQAGQRGLKVLLIDHAEKVAEKI
                     RISGGGRCNFTNRELDMRAPQRHFVGQNPQFCRSALSRYTPQDFIALVQRHGIAFHEK
                     HKGQLFADRSAEDIIAMLLAECADGGVERWQPCGVKNVVFYASSAAGESAGSYQINTD
                     FGPVQARSLVIATGGLSIPKIGASDLGYRIARQFGLPLVAQRPGLVPLTFDGADWAPY
                     AQLAGLALPVEISVGAKKERMAFHEDLLFTHRGLSGPAVLQISSYWQPGQPIAIDLAP
                     GVDLVDALQQAKARSKKRIANELATLVPSRLAEAWASTDADWQRPINEAADKALARLA
                     ERLARWQLTPMGTEGYKKAEVTLGGVDTRALSQQTMECKSQPGLYFIGEVVDITGWLG
                     GYNFQWAWASAAACAQALPAA"
     gene            2568..3059
                     /locus_tag="PK518_13115"
     CDS             2568..3059
                     /locus_tag="PK518_13115"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRM49896.1"
                     /translation="MNLPAHQLSMTVLMSPDMANFSGNVHGGAVLRLLDQVAYACASR
                     YSGCYVVTLSVDQVMFLQPIHVGELVTFLASVNHTGKSSMEIGIKVIAEDIRTQVVRH
                     VNSCFFSMVAVDDAGKPTPVSPFTPASDDERRRWDAALLRKALRHEQAQRHEQIRRAH
                     AAR"
     gene            complement(3060..3809)
                     /gene="cobA"
                     /locus_tag="PK518_13120"
     CDS             complement(3060..3809)
                     /gene="cobA"
                     /locus_tag="PK518_13120"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRM49897.1"
                     /translation="MTGSCTLVGAGPGDPELLTLKALKAIQAATVLFVDDLVSEAIVA
                     LAPAGARVEHVGKRGGCKSTPQADIEARMLAAVRAGETVVRLKGGDPFIFGRGGEEVE
                     HLRAAGIPVQVVNGITAGLAGMTLLGAPLTHRRHAQGVVFITGHSQPGGAGGDWRQLA
                     ATARDAHLTLVIYMGVASCARIQQELLTALPAHTPAAVIQHASLPQQRHAVTTLGQLQ
                     QTIAREGLGSPSIIVIGDVLRGIAAASAGQP"
     gene            complement(3806..4735)
                     /gene="ybiB"
                     /locus_tag="PK518_13125"
     CDS             complement(3806..4735)
                     /gene="ybiB"
                     /locus_tag="PK518_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HRM49898.1"
                     /translation="MGISHYIKEIGRGARGAKALDRTQAADLFGQVLDGQVSDLELGA
                     FCVAMRIKGESVDEMCGFLDAVQARIARFPACADGRPLIVLPSYNGARKLPVLTPLLA
                     LLLARAGQSVLLHGMRTEARRVLASNVLEALDIPALTAPEAIAPGSVAHIATGALLPG
                     LARLLAVREAIGLRTPGHSVAKLIAPCAGPALVVASYTHAEYFELLGATFGAMQMHAL
                     LSRGLEGEVAADPRRQPRYDGYVVGRHQLLQEQQGGTAAEVPGLPAEIDVDHTADYTR
                     RVLAGDAPVPVALQQQVDQILLLTRQITAQERP"
     gene            complement(4838..6076)
                     /locus_tag="PK518_13130"
     CDS             complement(4838..6076)
                     /locus_tag="PK518_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM49899.1"
                     /translation="MTGMRTFLKSGHAPTLFAAFLYFDFCFAVWVLNGAMAPFISEAY
                     QLNAAQKGFMISVPILAGALMRFPLGVLAQYIGRKNAAMVEMALIMAALALGFFFIRG
                     YDHVIAMGVLLGIAGASFGVALSLGSGWFPPQHKGLAMGIAGAGNSGTVLAVLFAPPL
                     AQAYGWQAVYGLAGLCMLVPFTVMAVFAKEPPDHEKQSLRQHVACLFERDGWAFSLLY
                     VVTFGGFIGLVSFLPTYFHDQFGVTKVQAGQLTMLAALMGSGVRVVGGYLGDRFGGVN
                     TLSSVLILVAATLVACGLAEGHLLATTLLFMLCFAALGAGNGALFQLVPLRWPLTTAV
                     AGSMIGEIGALGGGLVPNAMGLSKQYLGSYLWGFIAFALLALAMLAVLRVMQVRWTRT
                     WSSPGGRAPAASPRAAPATP"
     gene            complement(6271..>6527)
                     /locus_tag="PK518_13135"
     CDS             complement(6271..>6527)
                     /locus_tag="PK518_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRM49900.1"
                     /translation="RALLQPGAKAPVPLAARGANVCACEGVAENAITDLLQRCAGDAP
                     ARLQRLKDTLRCGTQCGSCVPQLQRMVQASLVQKPGNIET"
BASE COUNT         1078 a         2230 c         2225 g          994 t
ORIGIN      
        1 gctctacaac accagcgacg aggttgatcg cttcgtcgcc gccgtggccc atgccgtcaa
       61 ggtttttcaa tgagcactgc agaaaacgac ctctaccagg aagtcgtgct ggagcacaag
      121 cgtgcgccgc gcaactttgg ccagctcgcg caccccacgc accaggccca gggcaccaat
      181 ccatcgtgtg gcgaccgcgt gtcggtgcag ttgcagatgg acggccagcg cgtggccgac
      241 atccgcttca caggccaggg ctgcgccatc tgcatggcct ctacctcgat gatgaccgag
      301 gccgtcaagg gccgtgactt ggccgtggcg caagagctgc agcagcattt ccgcgccgtg
      361 ctcacgggcg aggaagaccc cgaggacgcg cccctgggca agctcgtcag cctggccggg
      421 gtgcgccaat accccagccg catcaaatgc gcgctgctgg gctggcacgc gctgatgcat
      481 gccattgccg acaacgacgg ccaggcggtg agcaccgaag aggagggcca gccatgatga
      541 accgccgcca ccgcgaagac gtgctggtgc gccgcaacgt gatggtcgag accatcccca
      601 gcggccaacc ggtggagctg gagcagggca tgctggcgca gatcacgcag gccctgggct
      661 cgtcgttcac gctgctggtc gagggccagc tgatgcgcct gaaagggctg gatgccgacg
      721 ccatcggcaa aaccccgccc gagaccattg ccgtgcccga ggatttgcac ctcgacgacc
      781 tggaaccgct ggtctgggag accctgcgca cctgctacga ccccgagatt cccgtcaaca
      841 tcgtcgatct gggcctggtt taccgcctcg aattccagcc ctccgacgaa gccgacaagg
      901 ttcgcgcggt gatcgacatg acgctcaccg cccccggttg cggcatgggc gaatccatcg
      961 ccaacgaggt ctgcgacaag gtgctggccc tgccacgcgt ggccgacatc accgtgaacc
     1021 tggtgttcga cccgccctgg gatcgctcca tgatgagcga agaggcgatg ctggcgctgg
     1081 gcctctgatc gtttcttgaa ttttctccct ccccctctgg gggagggcag gggtgggggc
     1141 cagcgacgct cgcgtgttga tgccggcaag atgccggcgc tccagccccc gttccaacct
     1201 tcgcccacgg gggggaggag tttgagacaa gctgctcatc aagaatcgat tacgcggccg
     1261 gcagcgcctg cgcgcaggcc gcggcgctgg cccaggccca ctggaagttg tagccaccca
     1321 gccagccggt gatgtccacc acctcgccga tgaagtacag gccgggctgg cttttacact
     1381 ccatggtctg ctgcgacagg gcgcgcgtgt ccacgccgcc cagtgtgacc tcggccttct
     1441 tgtagccctc agtccccatg ggcgtcagct gccagcgcgc caaccgctct gccaggcgtg
     1501 ccagggcctt gtcggcggcc tcgttgatgg ggcgctgcca gtcggcgtcg gtgctggccc
     1561 aggcctcggc caggcggctg ggcaccaggg tggcgagttc attggcgatg cgtttcttcg
     1621 atcgcgcctt ggcctgctgc aatgcatcga ccagatccac gcctggtgcc aggtcgatgg
     1681 caatcggctg gcctggctgc caatagctgg aaatctgcag caccgccggc cccgacaggc
     1741 cgcggtgcgt gaacagcagg tcttcgtgga aggccatgcg ctccttcttc gctcccacgc
     1801 tgatctccac cggcagcgcc aggccggcca gctgcgcata gggtgcccag tcggcaccgt
     1861 cgaaggtcag cggcaccagg cccgggcgct gcgccaccag cggcaggccg aactgccgcg
     1921 cgatgcggta acccaggtcg ctggcgccga tcttggggat ggacagaccg cccgtggcga
     1981 tcaccaggct gcgcgcctgc accgggccga agtcggtatt tatttgatag ctgccagcgc
     2041 tttcccctgc tgcgctactg gcataaaaga ccacattctt cacgccacag ggctgccagc
     2101 gctcaacgcc accatcggcg cattcggcca gcagcatggc gatgatgtcc tcggccgaac
     2161 gatcagcaaa cagctggccc ttgtgctttt catgaaaggc aatgccgtgg cgctgcacca
     2221 gggcgatgaa gtcctgtggt gtgtagcgcg acagggccga gcggcagaac tgcgggttct
     2281 ggcccacgaa gtgccgctgc ggggcgcgca tgtccagctc gcggttggtg aaattgcagc
     2341 gcccgccgcc cgagatacgg atcttctcgg ccaccttctc ggcatggtcg atgagtaata
     2401 ccttcagtcc gcgctggccc gcctgggcgg cgcagaacag gccggcggcg ccggcgccga
     2461 tgatgacggc atcaaagaat ggggtggaca cggggctgca ggggggtggg caaagccgga
     2521 cattgtcgcc cagcgcgcgc gtcgccctgg ctttctacaa tagccgcatg aacctgcccg
     2581 cgcaccagct cagcatgacc gtgctcatgt cgcccgacat ggccaatttc tccggcaacg
     2641 tccatggcgg cgccgtcctc cggctgctcg accaggtagc ctatgcctgc gccagccgct
     2701 actcgggctg ctatgtggtc acgctcagcg tggatcaggt gatgttcctg cagcccatcc
     2761 atgtgggtga gctcgtgacc ttcctggcca gcgtgaacca caccggcaag tcgtcgatgg
     2821 aaatcggcat caaggtcatt gccgaggaca tccgcaccca ggtggtgcgg catgtgaaca
     2881 gctgcttttt ctccatggtg gcggtggatg acgcgggcaa gcccacgccc gtgtcaccgt
     2941 tcacccccgc cagcgacgac gagcgccgcc gctgggacgc cgccctgctg cgcaaggccc
     3001 tgcgccacga gcaggcccag cgccacgagc agatccgccg cgcccacgcg gcgcgctgat
     3061 caaggctggc ccgcgctggc cgcggcgatg ccgcgcagca catcgccgat cacgatgatg
     3121 gacgggctgc cgagtccctc gcgcgctatg gtttgctgca gctgccccag cgtggtgacg
     3181 gcatggcgct gctgcggcag gctggcgtgc tgtatcaccg cggccggcgt gtgcgcgggc
     3241 agggcggtga gcagctcttg ctggatgcgc gcgcagctgg cgacgcccat gtagatcacc
     3301 agggtcaggt gggcatcgcg cgccgtggcg gccagctggc gccagtcgcc acccgcgccg
     3361 ccgggctggc tgtggccggt gatgaagacc acgccctgag catggcggcg gtgcgtgagc
     3421 ggcgcgccca gcagcgtcat gccggccagg ccggccgtga tgccgttgac cacctgcacg
     3481 gggatgccgg ccgcgcgcag gtgctccact tcctcgccgc cgcggccaaa gatgaagggg
     3541 tcgcccccct tgaggcgtac caccgtctcg ccggcgcgca cggccgccag catgcgcgcc
     3601 tcgatgtcgg cctggggcgt gctcttgcag ccgccgcgct tgcccacatg ctcgacgcgc
     3661 gcgccggccg gggccagggc cacgatggcc tcgctcacca ggtcgtccac gaacagcacc
     3721 gtggccgcct ggatcgcctt cagcgccttc agcgtcagca gctcggggtc gcccgggccg
     3781 gcgccgacca gggtgcagct gccggtcatg gccgctcctg cgccgtgatc tggcgcgtga
     3841 gcagcaggat ctgatcgacc tgctgctgca gcgccacggg cacgggcgca tcacccgcca
     3901 gcacgcggcg cgtgtagtcc gcggtgtgat caacgtcgat ctcggccggc aggcccggca
     3961 cctcggcggc cgtgccgccc tgctgctcct gcagcagctg gtgccggccg acgacgtagc
     4021 cgtcatagcg cggctggcgc cgtgggtcgg ccgccacctc gccctccagg ccgcgcgaga
     4081 gcagggcatg catctgcatg gcgccgaagg tggcgcccag cagctcgaag tactcggcgt
     4141 gcgtgtagct ggccaccacc agcgccgggc cggcgcaggg ggcgatcagt ttggccacac
     4201 tgtggccggg ggtgcgcagg ccgatcgcct cgcgcaccgc caggagacgc gccaggcccg
     4261 gcagcagcgc gccggtggcg atatgcgcca cgctgccggg tgctattgct tcaggagctg
     4321 tcagcgcagg tatgtcaagc gcttcaagca cattggatgc caacacccgg cgcgcctcgg
     4381 tgcgcatgcc gtgcagcagc acgctctggc cggcgcgcgc cagcagcagc gccagcaggg
     4441 gcgtgagcac gggcagcttg cgtgcgccgt tgtagctggg cagcacgatc agcggccgcc
     4501 cgtccgcgca ggcggggaac cgggcgatgc gcgcctgcac ggcatccaga aagccgcaca
     4561 tctcgtccac gctctcgccc ttgatgcgca tggccacgca gaaggcgccc agctccaggt
     4621 cgctgacctg gccgtccagc acctggccga acagatcggc cgcctgggtg cgatccagcg
     4681 ccttggcgcc gcgcgcgccg cggccgattt ccttgatgta gtggctgata cccatggtgt
     4741 ggtctgtgat gcagtggccg gccgattgtc ggtcaggatc gctatcggct ggcgcatgag
     4801 cttatgcctg ccagggcaca caggcgctgc cggggcttca cggcgttgcg ggcgccgccc
     4861 ggggggatgc agcaggggcg cgcccgcccg ggctgctcca ggtgcgggtc cagcgcacct
     4921 gcatgacgcg cagcacggcc agcatggcca gggccagcag ggcgaaggcg atgaagcccc
     4981 acaggtagct gcccaggtat tgcttggaca ggcccatggc gttgggcacc aggccgccgc
     5041 ccagggcgcc gatctcgccg atcatggagc cggccacggc cgtggtcagc ggccagcgca
     5101 gcggcaccag ctggaacagc gcgccgttgc ccgcgcccag cgcggcaaag cacagcatga
     5161 agagcagcgt ggtggccagc agatggccct cggccaggcc gcaggcgacc agcgtggcgg
     5221 cgaccaggat cagcacgctg ctcagggtgt tgacgccgcc gaagcggtcg cccagatagc
     5281 cgccgacgac gcgcacaccc gagcccatga gcgcggcgag catggtcagc tgcccggcct
     5341 gcaccttggt cacgccgaac tggtcgtgga agtaggtggg cagaaagctc accaggccga
     5401 tgaagccgcc gaaggtcacc acgtacagca ggctgaaggc ccagccgtcg cgctcgaaca
     5461 ggcaggcgac atgctggcgc aggctctgtt tttcatgatc cggcggctcc ttggcgaaaa
     5521 cggccatcac cgtgaagggc accagcatgc acaggcccgc caggccatag acggcctgcc
     5581 agccgtaggc ctgggccagc ggcggcgcaa acagcacggc cagcacggtg cccgaattgc
     5641 ctgcgcccgc gatgcccatg gccaggccct tgtgctgcgg cgggaaccag ccgctgccca
     5701 ggctcagcgc cacgccgaag ctggccccgg cgatgcccag cagcacgccc atggcgatca
     5761 catggtcgta gccgcggatg aagaaaaagc ccagcgccag cgccgccatg atgagcgcca
     5821 tctccaccat ggcggcgttc ttgcgcccga tgtactgggc cagcacgccc agcgggaaac
     5881 gcatcagcgc cccggccagg atgggcacgg aaatcatgaa gcccttctgt gcggcgttca
     5941 gttggtaggc ctcggaaatg aacggggcca tggcgccgtt gagcacccag accgcaaaac
     6001 agaaatcaaa atacaaaaag gccgcgaaca gcgtgggcgc gtggccggat ttgagaaagg
     6061 tgcgcattcc cgtcatggtg cttctccggt gggtcgttga aatggtgaga tggggcgtcg
     6121 tgggttcagg caccggctag tacagcaacc tggaagtagc ggaacaaaat gaaagcgatg
     6181 gattcgcgcc catggctagg cgcaaaccgc agcgatagcc gtagctatcg cgaggatttg
     6241 caacaccgac atgggtgtga agacgcgctt tcatgtttcg atatttccgg gtttctgtac
     6301 taggctggcc tgcaccatgc gctgcagctg cggcacgcag gagccgcact gcgtgccgca
     6361 acgcagcgtg tccttgagcc gctgcaggcg cgccggggcg tcgcccgcgc agcgctgcag
     6421 caggtcggtg atggcgttct cggccacgcc ctcgcaggcg cagacgttgg cgccgcgtgc
     6481 ggccagcggc acgggcgcct tggcaccggg ctgcagcagg gcgcggc
//
LOCUS       DAOMTQ010000161         6521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2739_length_6521_cov_21.651717, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000161 DAOMTQ010000000
VERSION     DAOMTQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2739_length_6521_cov_21.651717"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /gene="ffh"
                     /locus_tag="PK518_13140"
     CDS             <1..1247
                     /gene="ffh"
                     /locus_tag="PK518_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRM49901.1"
                     /translation="ALPVVRDFIARVKDKALGQEVLGSLKPGQALVGIVNKELAATMG
                     DGVADINLAAQPPAVILMAGLQGAGKTTTTAKLAKHLIEKRKKKVLTVSGDVYRPAAI
                     EQLKTVSKQAGAEWFASTPDQKPRDIALAALDYAKKHYFDVLLVDTAGRLAIDELLMQ
                     EIKDLHAVLNPVETLFVVDAMQGQDAVNTARAFKEALPLTGIVLTKLDGDSRGGAALS
                     VRQVTGAPIKFAGVSEKIDGLEVFDAERHAGRILGMGDIVALVEQVTAGVDMEAAQKL
                     AAKVKSGDGFDLNDFLAQLQQMKQMGGLSSLMDKLPAQLTAKAGQVDMDKAERDIRRK
                     EGIICSMTAQERRKPELIKATRKKRIAMGAGVQVQEVNRLLNEFDQMQGMMKKMKGGG
                     LMKLMKKMGGMKGMKGMPGMPF"
     gene            complement(1366..1668)
                     /locus_tag="PK518_13145"
     CDS             complement(1366..1668)
                     /locus_tag="PK518_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HRM49902.1"
                     /translation="MGTLKLRKWDSAEHLKTEEDMVLYLQACMEEAGDDAAFIAAALG
                     DIARAKGMSQLAKETGLGRESLYKALSGEGNPSFGTILKVMHALGLKLQPQAMGQA"
     gene            complement(1681..1971)
                     /locus_tag="PK518_13150"
     CDS             complement(1681..1971)
                     /locus_tag="PK518_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM49903.1"
                     /translation="MRTIHTTETFGDWFAGLRDKHAEKRIQARIRRAELGNFGDCAPV
                     GEGISEMRIHYGPGYRVYFTQRGMEIVILLAGGDKSTQTKDIKTALGLARQL"
     gene            complement(2195..2722)
                     /locus_tag="PK518_13155"
     CDS             complement(2195..2722)
                     /locus_tag="PK518_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49904.1"
                     /translation="MSVLPAPCISLNTAAVLTGRSVRTWQRRIEEGLVPRLGDERGRA
                     LVAWDAVRPALALRLDEAEVELLVRADQGDARAQAEVGALFALAALRDAKAAAPSNLG
                     GGSAMPALHFLTRAAEQGEADAMHWLGLLHAAGLGESGDGDALALMWVARAAAHGHAI
                     AARQLAGLLPAAAAV"
     gene            2950..3504
                     /locus_tag="PK518_13160"
     CDS             2950..3504
                     /locus_tag="PK518_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRM49905.1"
                     /translation="MKATLQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRTRVSEG
                     LVLAGAAKLAVSETFAANGGVAITGCNNPCTAAPVAGGTGYQFTATKYVQGMQIANIA
                     ATPVAGNGRVTINYSAATGMNGGALFIALTPGSGALVAATNLPTSPMTAGAPVVWGCT
                     TGTALGTPSGAAAMYKYVPANCRF"
     gene            3803..4513
                     /locus_tag="PK518_13165"
     CDS             3803..4513
                     /locus_tag="PK518_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRM49906.1"
                     /translation="MAALLVLWVWYPHPYGLLSGGRHLFLLLMGVDVVCGPLLTLVLF
                     NPTKPRRELLTDMALVVCIQLAALAYGLYTAQEARPLFLVHEVDRFRVVTAGDYGDVD
                     VRGAIGRLAAPLRPHLLRGPVTVGIREPKDAQEHQQVLMESVSGGRDYAQRPEFYLPY
                     DAAYAPRALARARPLPAFVKQFPATAGAVADLLGKQGVAMEDALFLPVLHKQEWVAVL
                     DKSARILGFVPGDGFAVP"
     assembly_gap    4539..4638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4832..5152)
                     /locus_tag="PK518_13170"
     CDS             complement(4832..5152)
                     /locus_tag="PK518_13170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM49907.1"
                     /translation="MVKRGEIWLVNLDPTVGSEIQKTRPCVVISPAELHDHLRTVIVA
                     PMTSKGFAAPFRVPVTHAGTKGLIVLDQLHTVDKMRLVKRLGTISGKTLNTTLTTLRE
                     IFEE"
     gene            complement(5146..5361)
                     /locus_tag="PK518_13175"
     CDS             complement(5146..5361)
                     /locus_tag="PK518_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRM49908.1"
                     /translation="MVLPKTLLAQAGLETVAVANVSVEQGAIVLRKPAKLARAGWADA
                     AKGIAAAGDDALLMGEFGNAADQDLTW"
     gene            5607..6161
                     /locus_tag="PK518_13180"
     CDS             5607..6161
                     /locus_tag="PK518_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRM49909.1"
                     /translation="MTVSSPPRHFIGEQEYLDGELHGELRHEYIDGQVYAMTGASDRH
                     GLILNALAYSMTPAARRKGCQLFTSDMKLRLEIGGKTCFYYPDLLLSCDPQDREPYFR
                     RSPCLIVEVLSESTARIDRREKLLAYQSLPSLQAYLLVEQEARRVELYRRATDWRVEY
                     HDAGEIWLDCLELALPLAEVYADV"
     gene            complement(6334..>6521)
                     /locus_tag="PK518_13185"
     CDS             complement(6334..>6521)
                     /locus_tag="PK518_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRM49910.1"
                     /translation="LVEGLSKRDGNELMGRTECNRVVNFPGHERLIGQLIDVKITEAR
                     TYTLRGEVVMQESGQSL"
BASE COUNT         1126 a         1915 c         2090 g         1290 t
ORIGIN      
        1 tggccctgcc cgtggtgcgc gacttcatcg cccgcgtcaa ggacaaggcc ctgggtcagg
       61 aggtgctggg cagcctcaag cccggccagg cgctggtggg catagtcaac aaggaactgg
      121 ccgccaccat gggcgacggc gtggccgaca tcaatctggc cgcccagccc cccgccgtca
      181 tcctgatggc cggcctgcag ggcgcgggca agaccaccac cacggcaaaa ctggccaagc
      241 acctgataga gaagcgcaag aagaaggtgc tcaccgtctc gggcgacgtg taccgcccgg
      301 cggccataga gcagctcaag accgtcagca agcaggccgg cgccgagtgg tttgccagca
      361 cgcccgacca gaagccgcgc gacatcgccc tggccgcgct ggactacgcc aaaaagcatt
      421 acttcgacgt gctgctggtc gataccgccg gccgcctggc catcgatgaa ctgttgatgc
      481 aggagatcaa ggacctgcat gccgtgctga acccggtcga gaccctgttc gtggtggatg
      541 ccatgcaggg ccaggacgcg gtgaataccg cccgcgcctt caaagaggcg ctgccgctca
      601 ccggcatcgt gctgacaaaa ctggacggcg actcgcgcgg cggcgcggcg ctgtcggtgc
      661 gccaggtgac gggcgcgccc atcaagttcg ccggcgtgtc cgagaagatc gacggcctgg
      721 aggtcttcga cgccgagcgc cacgccggcc gcatcctggg catgggcgac atcgtggccc
      781 tggtcgagca ggtgacggcc ggggtggaca tggaggcggc gcaaaagctc gccgccaagg
      841 tcaagagcgg cgacggcttc gacctgaacg actttctggc ccagctgcag cagatgaagc
      901 agatgggcgg gctgtcgagc ctgatggaca agctgcccgc ccagctgact gccaaggccg
      961 gccaggtgga catggacaag gccgagcgcg acatccgccg caaggagggc atcatctgca
     1021 gcatgaccgc ccaggagcgc cgcaagcccg aactcatcaa ggccacgcgc aagaagcgca
     1081 tcgccatggg cgccggcgtg caggtgcagg aggtcaaccg cctgctcaat gagttcgatc
     1141 agatgcaggg catgatgaag aagatgaagg gcggcggcct gatgaagctg atgaagaaga
     1201 tgggcggcat gaagggcatg aaaggcatgc ccggcatgcc tttctagccg gtggccagcc
     1261 ggcatcaatg caaaaacaat agctgcttgc gcttgctggt aaagcgctag agctattttt
     1321 ttgttttttt gactagaacg cggtgcaggc tggcgccacc gcgtttcagg cctggcccat
     1381 ggcctggggc tgcagcttca agcccagtgc gtgcatcacc ttcaggatgg tgccaaaact
     1441 cgggttgccc tcgcccgaca gtgccttgta caggctctcg cgccccaggc ccgtttcctt
     1501 cgcgagctgg ctcatgccct tggcgcgggc aatgtcgccc agggcagccg cgatgaaggc
     1561 ggcgtcatcg ccggcttcct ccatgcaggc ttgcaggtac agcaccatgt cctcctcagt
     1621 cttgagatgc tcggcactgt cccatttacg cagtttcagg gttcccatgg tggcttctcc
     1681 ttacagttgc cgcgccagcc ccagcgcggt cttgatgtcc ttggtctggg tggacttgtc
     1741 gccaccggcc agcaggatca cgatctccat gccgcgctgt gtgaaataca cgcgatagcc
     1801 cggcccgtag tgaatccgca tttccgatat gccttcgcca accggtgcgc aatcgccgaa
     1861 gttgcccaac tccgcccggc gaattcgtgc ctgtatgcgt ttttcggcgt gcttgtcccg
     1921 cagtcccgca aaccagtcac cgaatgtctc ggtggtgtgg atggttcgca tgcgtcaatt
     1981 gtataaaaca agatacaaat aatccagcat ccggctaaac catcatggaa acatggaagg
     2041 cctgcaccat tgcgctgagc ggttgcgccg gttttacccc ctctccctct gggagagggc
     2101 aggggtgagg gcaggcaacc gtatttgatg cgtattgcaa atcgggcaaa aaccgcctca
     2161 acccatgcag attaagcgca accagctatc gtttttaaac cgctgccgct gccggcagca
     2221 aacccgccag ctgccgcgcc gcaatcgcat gcccgtgcgc tgcggcccgg gccacccaca
     2281 tcagcgccag ggcgtcgccg tcgccggact cgcccaaccc ggcggcgtgc agcaggccca
     2341 gccagtgcat ggcgtccgcc tcgccctgct cggcggcccg cgtgagaaag tgcagcgcgg
     2401 gcatggcgct gccccctccc agattggagg gcgcggctgc cttggcgtcg cgcagggccg
     2461 ccagtgcgaa tagcgcgccc acctcggcct gggcccgggc gtcgccctgg tcggcgcgca
     2521 ccagcagctc cacctcggcc tcgtccagcc gcagcgccag cgccggccgc accgcatccc
     2581 acgccaccag cgcccgcccg cgctcgtctc ccaggcgcgg caccagcccc tcctctatgc
     2641 gccgctgcca ggtgcgcacg ctgcgcccgg tgagcacggc ggcggtgttc aggctgatgc
     2701 agggggcggg aagtacggac atgcgccgat tctatggggc gttccccatg ggtcgtgtcg
     2761 cgcaaaattt gcgacaaaaa gtgtgcaggt gtcgctggat ggcggcgacg taactttacg
     2821 tgtctatgtg ttacggcgac agggcgccgc tttttgccgt ttttgcgccg ttttcaggct
     2881 ggcacgccgc ttgcgctagg tacttcgccc aggcctggtg cctgtttttt tctgaatgag
     2941 ggacttgtga tgaaagcaac tttgcaaaag ggtttcaccc tgatcgaact gatgatcgtg
     3001 gtggcgatca ttggtatttt ggctgccgtg gcgttgccgg cttatcagga ctacacggtg
     3061 cgtacccgcg tgagcgaggg cctggttttg gctggggcgg caaagctggc tgtgtcggag
     3121 actttcgccg ccaatggagg ggttgcaatt acgggatgta ataatccctg tactgcggct
     3181 cctgtggctg gtggtaccgg ctaccagttc acggcaacca agtatgttca aggtatgcag
     3241 attgctaaca ttgcggccac tcctgtggct ggcaatggcc gcgtcactat caactacagc
     3301 gcagcaactg gcatgaatgg cggtgcattg tttattgctc tgacgcccgg gtcgggtgct
     3361 ttggttgccg ctaccaatct tcccaccagc cctatgactg caggtgcgcc ggttgtttgg
     3421 ggttgcacca cgggtaccgc gctaggtacg ccgagtggcg cagccgcgat gtacaagtac
     3481 gttcctgcaa actgccgctt ctgagtttgc attcacagag caagggccac tgcggtggcc
     3541 cttttgtttt gtcaatggat ggcttttaac ggggccggga gaggtggcca ggtcttcggc
     3601 atgtaatcga cgtagtgttg gttccgggca gacaagaatg catgcgacaa gcagttggga
     3661 tgcctgaacg caaggggatc tgcctgcgat ccaactggcg tagcagatgt atggttgaga
     3721 aatggtgata tccaagcgac gcgcacgggg tgcgactaag gcggcatttg tccattttct
     3781 ggggtcgata tgcgtggcga gcgtggccgc cctgctggtg ctctgggtct ggtacccgca
     3841 cccctatggc ctgctgtcgg gcgggcgcca tctgttcctg ctgttgatgg gggtggatgt
     3901 ggtgtgcggg ccgctgctca ccctggtgtt gttcaacccg accaaacccc ggcgcgagct
     3961 gttgacggac atggcgctgg tggtgtgcat tcagcttgcg gcgctggcct atgggctgta
     4021 cacggcgcaa gaggcacggc ccttgttcct ggtgcatgag gtggatcgct tccgcgtggt
     4081 aaccgcgggc gactatggtg atgtggatgt acgcggcgcc atcggccgtc tggctgcgcc
     4141 gctgcgcccg catctgctgc gtggcccagt gacggtgggc attcgcgagc ccaaagatgc
     4201 ccaggagcat cagcaggtgt tgatggagtc cgtgtcaggc gggcgcgact acgcgcagcg
     4261 ccccgagttc tacctgccct acgacgccgc ctacgccccc agggcgctgg cgcgcgcccg
     4321 gcccctgcca gccttcgtca agcagtttcc ggcgaccgcc ggcgctgtgg ccgacttgct
     4381 ggggaagcag ggggtggcta tggaggatgc gctgttcctg cccgtgctgc acaagcagga
     4441 atgggtggcg gtgctggaca agtccgcccg cattctgggc tttgtgccgg gcgacgggtt
     4501 tgccgtgccg tagtgcggcg gcaggccagg gtgttttgnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnngt gccgggcgac gggtttgccg tgccgtagtg cggcggcagg
     4681 ccagggtgtt ttgtgcataa aaatagctgc tagcgcttta ccataaagcg tgagcagcta
     4741 tcttttttgt aattggggtc gtattccaat ttactcttca aaaatctctt gcaaggtggt
     4801 cagagtgggg taattggggt cagattccaa tttactcttc aaaaatctct cgcaaggtgg
     4861 tcagagtggt attcagggtc ttgcccgaaa tcgtccccag ccttttcacc agccgcattt
     4921 tgtccacggt gtgcagctga tcgagcacga tcagcccctt ggtgccggcg tgggtgacag
     4981 gcacgcgaaa aggcgcggca aagcctttgg aggtcatggg cgcgacgatg accgtgcgca
     5041 agtgatcgtg cagttccgca ggagaaatca ccacgcaggg ccgcgtcttt tgaatctcgc
     5101 tgcctaccgt gggatccagg ttgaccagcc agatctctcc acgcttcacc acgtgagatc
     5161 ctggtctgcg gcgttgccaa actcacccat cagcaatgca tcgtcacccg ctgcggcgat
     5221 gcccttggcg gcatctgccc agccggcgcg agccagtttg gcaggtttgc gcagcacgat
     5281 ggcgccttgc tcgaccgaga cattggccac tgcgacggtt tccaaccctg cctgggcgag
     5341 caaggtcttg ggcaacacca tgcctttgct gttgcctatg gggtaattgg ggtcagactc
     5401 caattgtttg ctgaaaactg gccataaggc ttgctgtttg ggcggtagta gctcttcttt
     5461 ttgatgtgat tggccgtgtt ttgctccagc gggcgaggtg gcgttagacc tcgccaagat
     5521 cagacgccac ccatgcggtt gggtttcggg actgcatggc ggccgatcgg gcgtagactg
     5581 cagcgcatgc gaggaaaggg gttcccatga cggtatcgag cccacccagg catttcattg
     5641 gcgagcagga gtatctggac ggcgagctgc atggtgagct gcgccatgag tacatcgacg
     5701 ggcaggtgta tgcgatgacc ggggccagcg accggcatgg cctgatcctg aatgccctgg
     5761 cctactcgat gacgccggcg gcgcggcgca agggctgcca gctgttcacg tccgacatga
     5821 agctgcggct ggagattggc ggcaagacct gtttttatta cccggatctg ctgctgtcct
     5881 gcgacccgca ggatcgggag ccgtatttca ggcgctcgcc ctgcctgatc gtcgaggtgc
     5941 tgtcggaatc taccgcgcgc atcgatcggc gcgaaaagct gctggcctac cagagcctgc
     6001 ccagcctgca ggcgtatctg ctggtggagc aggaggcgcg gcgcgtggag ctgtaccgcc
     6061 gcgccaccga ctggcgcgtg gagtaccacg acgcgggcga gatctggctg gactgcctgg
     6121 agctggcgct gccactggcc gaggtgtatg cggatgtgtg atgtttttgg ggtcagtacc
     6181 tctcagttaa caggtttgta ggagcggctt cagccgcgaa tgacgccgtt cgcggctgaa
     6241 gccgctccta cacacagatg gcatcggttg ctgtctgaaa agtattgttt agtgcgcaag
     6301 cacagacagt gcaatcgcct aatgctgcgt cttttatagt gactgacccg actcctgcat
     6361 caccacctcg ccgcgcaggg tgtaggtgcg tgcctcggtg atcttcacgt ctatcagttg
     6421 gcctatcagg cgttcgtggc cggggaagtt gaccacgcgg ttgcattcgg tgcggcccat
     6481 gagctcgttg ccgtcgcgtt tggagaggcc ctcgaccagc a
//
LOCUS       DAOMTQ010000162         6502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2758_length_6502_cov_26.119435, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000162 DAOMTQ010000000
VERSION     DAOMTQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_6502_cov_26.119435"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PK518_13190"
     CDS             complement(<1..833)
                     /locus_tag="PK518_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRM49911.1"
                     /translation="MKLRTFLTTAVAAAAALAFSAPALAQSNYKSEYRMSLVLGPAFP
                     WGKGGELWAAKVKEKTQGRINIKLYPGVSLIQGDQTREFSALRQGVIDMAIGSTINWS
                     PQVKQLNLFSLPFLFPNYAAVDAVTQGEVGASIFKTLDKAGVVPLAWGENGYREISNS
                     KHAIKSPADLKGLKIRVVGSPLFLDTFTALGANPTQMSWADAQPAFASGAVDGQENPL
                     SIFTAAKLHNVAQKHITMWGYMNDPLVFVVNKDVWNSWTPADREAVKQAAIEAGKEQI
                     A"
     gene            complement(858..2156)
                     /locus_tag="PK518_13195"
     CDS             complement(858..2156)
                     /locus_tag="PK518_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRM49912.1"
                     /translation="MIATLLFSAFLLLMFLGVPIGAALGLAGAAAIALANAETQWFGL
                     LAVPQNFYAGLGKYPLLAIPMFVLVGSIFDRSGVALRLVNFAVSIVGRGPGMLPLVAI
                     SVAMFMGGISGSGPATAAAVGAVMIAAMARAGYPGAFSASVVAAAAATDILIPPSVAF
                     IVYSVLVPGASVPALFAAGMFPGILAGLALIIPAVWLARRHKMGRLEASLPRPPFWRS
                     LREAAWGLAAPVVILGGMRMGWFTPTEAAVVAVFYGLFVGMVVHRTIGVRDLFPILRE
                     AGELSAVILIVVSLAGIFAFSLSTLGVIDPVTRFIVESGMGETGVMAVLMVLLLIMGM
                     FLDGISIMLIFVPLVLPLVQHFQWDVVWFGTVLVLTIAIGQFTPPMAVNLMVASKIAQ
                     VRMEATTRWVLWLVLAMCLAMLAVVAWPQIALWLPAKLGY"
     gene            complement(2153..2710)
                     /locus_tag="PK518_13200"
     CDS             complement(2153..2710)
                     /locus_tag="PK518_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRM49913.1"
                     /translation="MDSTTSAPATPPVPSPEEPRSLRLEDWLTVLIMALLALITFANV
                     IVRYFTDSSFAWTEEISVFLMIALALVAGSAAVARDQHIRIEFFCQSGTALRRRRLAQ
                     LGAACVALLFATIAVLSVRVVWDDYRFEETSPGIGVPQWWYSIWLPVLSALITWRAIG
                     LLRRRTRQQDSEYLAQDAQQPEVSP"
     gene            2832..3770
                     /locus_tag="PK518_13205"
     CDS             2832..3770
                     /locus_tag="PK518_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM49914.1"
                     /translation="METKWLEDFVSLAETRSFSRSAQLRHVTQPAFSRRIQALEAWAG
                     TDLVDRSSYPTRLTAAGTTLYDQALEVLQALQNTRAMLRGHSSSSSDMIEFAVPHTLA
                     FTFFPAWVSAVRADFGPLKSRLIALNVHDAVMRLVEGGCDLLISYHHPSQPLQLDPER
                     YDMVTLGQEVLAPYSKAGPDGQPLFCLPGSVGEPLPYLGYAPGAYLGRVTELILKQSG
                     TTIHLERVYETDMAEGLKAMALEGHGVAFLPYSAVKKELRAHKLVSAAPPGVEDWQMI
                     MEVRAYREKPSRKESAKGLAQALWAYLQAQGGQNPI"
     gene            3966..4865
                     /locus_tag="PK518_13210"
     CDS             3966..4865
                     /locus_tag="PK518_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM49915.1"
                     /translation="MKKHLLAAAITVLAAGSALAQANDTLAKIKSSGAVTLGVRESSG
                     LGYTLGSGKYVGFHTEMGERILADIQKQLGLAKMEIKYQPVTSQNRIPLVVNGTVDIE
                     CGSTTNNMARQKDVAFAYTTYVEEVRIAVNAKSGITGIKDLNGKTIVTTTGTTSVQTL
                     RKNKRADGLTFKEVMGKDHADSFLMLETGRADAFIMDGSILAANISKSKNPADYKVVG
                     EVLSVEPIACMLRKDDPAFKKAVDDSIVRQIKDGSLAKLYDRWFMQPIPPNNVKVGLP
                     LSEATKEAWANPNDKPMESYEVK"
     gene            4980..5732
                     /locus_tag="PK518_13215"
     CDS             4980..5732
                     /locus_tag="PK518_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM49916.1"
                     /translation="MSWDWQVFCQDTITQEVGASCFGQGGDITYLDWMLSAWGWTVSV
                     SLLSLIVALVVGSVIGTLRTLPDSPWIVRLGNAWVEFFRNIPLLVHIFLWYHVIPSIF
                     PAMKSLPGFVLVVFALGFFTSARIAEQLRSGILALPRGQRYAGMAMGFTTFQYYRYVL
                     LPMAFRIIIPPLTSETMNIFKNSSVAFAVSVAELTMFAMQAQEETSRGIEVYLAVTGL
                     YVISALAINRIMAFVEKRSRVPGMIAAGGGGH"
     gene            5734..6405
                     /locus_tag="PK518_13220"
     CDS             5734..6405
                     /locus_tag="PK518_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM49917.1"
                     /translation="MNLNIDWSFFTWDLFTNFVLKGLYFSLFLTVVATAGGIALGTVL
                     ALMRLSGKKWLDLPATIYVNGMRSIPLVMVILWFFLLVPLIIGRPIGAEVSAIITFVA
                     FEAAYFSEIMRAGIQSIPRGQVFAGQAMGMTYGQNMKLVVLPQAFRNMLPVLLTQTII
                     LFQDTSLVYAIGAYDMLKGFEIAGKNYGRPIEAYMAAAVVYFIICYALSWSVKRLHKK
                     IAIIR"
BASE COUNT         1191 a         2104 c         2118 g         1089 t
ORIGIN      
        1 atggcgatct gctccttgcc ggcctcgatg gcggcctgct tgacggcctc acggtcggcc
       61 ggtgtccagc tgttccagac atccttgttc accacgaaga ctagcgggtc gttcatgtag
      121 ccccacatgg tgatgtgctt ttgcgccacg ttgtgcagct tggcggcagt gaagatggac
      181 agggggtttt cctgtccgtc cacggcgccg ctggcgaagg ccggctgggc gtcggcccag
      241 ctcatttgcg tggggttggc gcccagggcg gtgaaggtgt ccaggaacag cggcgagccg
      301 acgacgcgga tcttcaggcc cttcaggtcg gccgggcttt tgatggcgtg cttggagttg
      361 ctgatctcgc ggtagccgtt ctcaccccag gccagcggca cgacaccggc cttgtccagg
      421 gtcttgaaga tgcttgcgcc cacctcgccc tgcgtgactg catcgacggc ggcgtaattg
      481 gggaacaaaa agggcagcga gaacaggttc agctgcttga cctggggcga ccagttgatg
      541 gtcgagccaa tggccatgtc gatcacgccc tggcgcagcg cgctgaattc acgcgtctgg
      601 tcgccctgga tcagcgacac gccgggatag agcttgatgt tgatgcggcc ctgggtcttt
      661 tccttgacct tggcggccca cagctcgccg cccttgcccc aggggaaggc cgggcccagc
      721 accagcgaca tgcggtattc gctcttgtag ttgctctgcg ccagcgcggg cgcggaaaaa
      781 gccagcgcag cggcggcggc gacggcggtg gtcagaaagg tacgcagttt catcgtcaaa
      841 gtctccttgg gaaaaaatca ataccccagc ttggcgggca gccacagcgc gatctgcggc
      901 caggccacca cggccagcat ggccaggcac atggccagca ccagccacag cacccagcgc
      961 gtggtggctt ccatgcgcac ctgggcgatt ttcgaggcca ccatcaagtt gacggccatg
     1021 ggcggggtga actggccgat ggcgatggtg agcaccagca cggtgccgaa ccacaccacg
     1081 tcccactgga aatgctgcac cagcggcagc accagcggca cgaagatgag catgatggag
     1141 atgccatcga ggaacatgcc catgatgagc agcagcacca tgagcacggc catgacgccg
     1201 gtctcgccca tgccggattc gacgatgaag cgcgtcaccg ggtcgatcac gcccagggtg
     1261 gacagcgaga acgcaaagat gcctgccagc gacaccacga tcaggatcac cgccgacagc
     1321 tcgcccgcct cgcgcaggat gggaaacagg tcgcgcacgc caatcgtgcg gtgcaccacc
     1381 atgcccacga acaggccgta gaacacggcc accacggcgg cctcggtggg tgtgaaccag
     1441 cccatgcgca tgccgcccag gatcaccacc ggcgcggcca ggccccaggc ggcctcgcgc
     1501 aggctgcgcc agaacggcgg gcggggcagg ctggcctcca gccggcccat cttgtggcgc
     1561 cgcgccagcc acacggccgg gatgatgagc gccagccccg ccaggatgcc ggggaacatg
     1621 ccggccgcaa acagtgccgg caccgatgcg ccgggcacca gcaccgaata gacgatgaag
     1681 gccaccgagg gcggaatcag gatgtcggtg gccgcggcgg ccgccaccac gctggcagaa
     1741 aacgcaccag ggtagccggc gcgtgccatg gcggcgatca tcaccgcgcc cacggcggcg
     1801 gccgtggccg ggcccgagcc ggagatgccg cccatgaaca tggccacgct gatggccacc
     1861 agtggcagca tgcccggccc gcgcccgact attgatacgg caaagttcac cagccgcagc
     1921 gccacgcccg aacggtcgaa gatggagccc acgagcacga acatcgggat ggccagcagc
     1981 gggtacttgc ccagcccggc gtagaagttc tgcggcacgg ccagcaggcc gaaccactgg
     2041 gtctcggcat tggccagcgc aatcgccgcc gcgccggcca ggcccagggc cgcgccaatc
     2101 ggcacgccca gaaacatcag cagcaaaaac gcgctgaaga gcagggtggc gatcatggcg
     2161 acacctccgg ctgctgcgcg tcctgcgcca ggtactcgga gtcctgctgg cgcgtgcgcc
     2221 ggcgcagcag gccgatggca cgccaggtga tcagcgctga cagcaccggc agccagatcg
     2281 agtaccacca ctgcggcacg cctatgccgg gcgaggtttc ctcaaaacgg taatcgtccc
     2341 agaccacgcg cacactgagc acggcaatcg tggcaaacag cagcgccacg caggccgcgc
     2401 ccagctgcgc caggcgccgg cggcgcagcg ccgtgccgct ctggcagaaa aattcgatgc
     2461 ggatgtgctg gtcgcgtgcc acggcggccg agcccgccac cagggccagg gcgatcatca
     2521 ggaacaccga gatttcctcg gtccaggcga aggacgaatc ggtgaagtag cgcacgatca
     2581 cgttggcaaa ggtgatcagt gcaagcagag ccatgatgag gacggtgagc cagtcctcca
     2641 ggcgcaggct gcgcggctcc tccgggctgg ggacgggggg cgtggctggg gcagaagtgg
     2701 tggaatccat gaacgctggg cagaggcagg acagacggct gggcgccgca cggccgggcg
     2761 attatgcgcg ctgctatgga aactatgtct tttgcgcagc ccatgcccgc gcgggcggat
     2821 aatgccgcgc catggaaacc aaatggcttg aagacttcgt cagcctggct gaaacccgca
     2881 gcttcagccg ctcggcacag ctgcgccatg tcacgcagcc ggcgttctcg cgccgcatcc
     2941 aggcgctgga ggcctgggcg ggcacggatc tggtggatcg cagctcctac cccacgcggc
     3001 tcaccgccgc cggcaccacg ctgtacgacc aggcgctgga ggtgctgcag gcgctgcaga
     3061 acacacgcgc catgctgcgc gggcattcca gcagcagctc cgacatgatc gagttcgccg
     3121 tgccgcacac gctggccttc accttcttcc cggcctgggt gtcggcggtg cgcgcggact
     3181 tcgggccgct caagagccgg ttgatcgcgc tcaacgtgca cgacgccgtg atgcgcctgg
     3241 tggagggcgg gtgcgacctg ctgatctcct accaccaccc gtcgcagccg ctgcagctcg
     3301 accccgagcg ctacgacatg gtgaccctgg gccaggaggt gctggcgccg tactccaagg
     3361 ccggccccga tggccagccg ctgttctgcc tgcccgggag cgtgggcgag ccgctgccct
     3421 atctgggcta cgcgccgggc gcctatctgg gccgtgtcac ggagctgatc ctcaagcagt
     3481 cgggcaccac catccacctg gagcgggtgt acgagaccga catggccgaa ggcctgaagg
     3541 ccatggcgct cgaaggccat ggcgtggcct ttctgcccta cagcgccgtg aagaaggagc
     3601 tgcgcgcgca caagctggtg agcgccgccc cgcctggcgt ggaagattgg cagatgatca
     3661 tggaagtgcg cgcctaccgt gaaaaacctt cgcgcaagga gtcggccaag ggcctggctc
     3721 aggcgctgtg ggcctatctg caggcccagg gcgggcaaaa ccccatctga ttgaacttgg
     3781 gcgctgttct ggaaataccc taggaatctt gcgccccggg aggcggtgga gcacgcacaa
     3841 tacgaggcct gtgctccccg ttcgggagcg ggttgcactt ggggcacgcg ccattggcga
     3901 tctgcgccgc catgcaaccc atgaaccaca gcccaatcat cgtttcaccc acaaggagat
     3961 cccacatgaa gaaacatttg ctggccgccg cgatcaccgt tctggctgca ggcagcgccc
     4021 tggcgcaggc caacgacacc ctggcgaaga tcaaatcgtc tggcgccgtc accctgggcg
     4081 tgcgcgaatc atcgggcctg ggctacacgc tgggcagtgg caagtacgtg ggttttcaca
     4141 ccgagatggg tgagcgcatc ctggccgaca tccagaagca gctgggcctg gccaagatgg
     4201 agatcaagta ccagcccgtg acctcgcaga accgcatccc gctggtggtc aacggcaccg
     4261 tggacatcga gtgcggctcc accaccaaca atatggcgcg ccagaaggac gtggcctttg
     4321 cctacaccac ctatgtggaa gaggtgcgca tcgcggtgaa tgccaagtcg ggcatcaccg
     4381 gcatcaagga tctgaatggc aagaccatcg tcaccaccac cggcacgacc tctgtgcaga
     4441 cgctacgcaa gaacaagcgc gccgatggcc tgaccttcaa ggaggtcatg ggcaaggacc
     4501 acgccgacag cttcctgatg ctggagacgg gccgcgccga cgccttcatc atggacggct
     4561 ccatcctggc ggccaacatc tccaagtcca agaacccggc cgactacaag gtcgtcggcg
     4621 aggtgctgtc ggtcgagccc atcgcctgca tgctgcgcaa ggatgatccg gccttcaaga
     4681 aggccgtgga cgactccatc gtgcgccaga tcaaggacgg ctcgctggcc aagctctacg
     4741 accgctggtt catgcagccc atcccgccga acaacgtgaa ggtcggcctg ccgctgtccg
     4801 aggccaccaa ggaagcctgg gcgaacccga acgacaagcc catggagtcc tacgaagtca
     4861 agtaagcggc gctgcacata ggctggcccc ttctgacctg atggacggaa ggggcctttt
     4921 tgttgcgcac cgcaatgcgg gccgcgcatg tccgagttaa caaacaaaga ggtgttctta
     4981 tgagttggga ttggcaggtg ttctgccagg acaccattac ccaggaggtg ggcgcgagct
     5041 gcttcggcca gggtggtgac atcacctatc tggactggat gctgtcggcc tggggctgga
     5101 ccgtgtcggt gtctctgctg tctttgatcg tggcgttggt cgtgggatcg gtgattggta
     5161 ccttgcgcac cctgcccgat agtccgtgga tcgtgcgcct gggcaatgcc tgggtcgagt
     5221 tcttccgcaa tattccgctg ctggtacaca tttttctgtg gtaccacgtg atcccgtcga
     5281 tcttcccggc catgaagtcg ctgcccggct ttgtgctggt ggtgtttgcc ctgggcttct
     5341 tcacctcggc gcgcattgcc gagcagctgc gctcgggcat tctggcgctg ccgcgcggcc
     5401 agcgctacgc cggcatggcg atgggcttca ccaccttcca gtactaccgc tatgtgctgc
     5461 tgccgatggc gtttcgcatc atcatcccgc cgctgaccag cgagaccatg aacattttca
     5521 agaactcgtc ggtggccttt gccgtgtcgg tggccgagct gaccatgttc gccatgcagg
     5581 cgcaggagga aacctcgcgc ggcatcgagg tctatctggc ggtgaccggg ctgtacgtga
     5641 tctcggcctt ggccatcaac cgcatcatgg ccttcgtcga gaagcgctcg cgcgtgcccg
     5701 gcatgattgc tgcgggcggg ggagggcatt gatatgaacc tgaatatcga ttggtccttc
     5761 ttcacctggg atctgttcac caacttcgtg ctcaaggggc tgtacttcag cctgtttctg
     5821 accgtggtgg ccacggctgg cggcattgcc ctgggtacgg tgctggcgct gatgcggctg
     5881 tcaggcaaga agtggctgga ccttccagcc accatctacg tcaacggcat gcgctccatc
     5941 ccgctggtga tggtgatcct gtggttcttc ctgttggtgc cgctgatcat cgggcggccc
     6001 ataggtgccg aggtctcggc catcatcacc ttcgtggcct tcgaggcggc ctacttcagc
     6061 gagatcatgc gcgccggcat ccagtccata ccccggggcc aggtgtttgc cggccaggcc
     6121 atgggcatga cctatgggca gaacatgaag ctggtggtgc tgccgcaggc gtttcgcaac
     6181 atgctgccgg tgctgctcac gcagaccatc atcctgttcc aggacacctc gctggtctat
     6241 gccattggcg cctatgacat gctcaagggc ttcgagatcg ccggcaagaa ctatggccgc
     6301 ccgatcgaag cctacatggc ggcggccgtg gtctatttca tcatttgcta cgcgctgtcg
     6361 tggagcgtca agcgcctgca caagaagatc gccatcatcc gctgatatgc agcacaaccc
     6421 cctgaggcgc tgcgcgcctt cccccttctc tcgcggcgct gcgcgccgcg ggaaggggga
     6481 cgccaccagc gcggcggggc gg
//
LOCUS       DAOMTQ010000163         6495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2762_length_6495_cov_26.108540, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000163 DAOMTQ010000000
VERSION     DAOMTQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2762_length_6495_cov_26.108540"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..101
                     /locus_tag="PK518_13225"
     tRNA            17..101
                     /locus_tag="PK518_13225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Leu,seq:caa)
     gene            complement(719..946)
                     /locus_tag="PK518_13230"
     CDS             complement(719..946)
                     /locus_tag="PK518_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49918.1"
                     /translation="MATAQPMYHAPTFEPTVDEIATLKQLEMGEAIAVPDALKHHLSG
                     RLLDWGYIGKSPTGVFHITDTGRKLIRRQDN"
     gene            1086..1231
                     /locus_tag="PK518_13235"
                     /pseudo
     CDS             1086..1231
                     /locus_tag="PK518_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466196.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
     gene            complement(1254..2207)
                     /locus_tag="PK518_13240"
     CDS             complement(1254..2207)
                     /locus_tag="PK518_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM49919.1"
                     /translation="MRRHLPSTQALACFEAAARHESYTRAAQELALTQSAVSRQILAL
                     EDQLGVQLFRRTRHGVVLTPAGRHYARQVARWLQALEQGTLDVMAHQGQGGAISLAAV
                     ATFATRWLIPRLPQLAARHPEIVVHIETQTRPFLFTDTGFDAALYAGTPEQVANWPGV
                     QAQLLMPEDVLPVCSPRLLEQAAEQSGMPRGTGRAHQGLAPQLLARMPLLQQSTRPYG
                     WQQWFEAMGVQDAPRALDGPRYELFSMTAVAAAQGLGVALIPPMLIEAEMARGELVVA
                     CARPLRGERGYYLVSPAQPQPPALAAFARWLQDMVAAGPVA"
     gene            2373..3569
                     /locus_tag="PK518_13245"
     CDS             2373..3569
                     /locus_tag="PK518_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRM49920.1"
                     /translation="MTRAAFQWDDPFHLDQQLTEDERAIRDAAHAYCQDKLAPRVLEM
                     FRHEKTDVSIFREMGELGLLGPTIPTEYGGAGLNYVSYGLVAREIERVDSGYRSMASV
                     QSSLVMVPINEFGSEAQKQKYLPKLASGEYIGCFGLTEPDHGSDPGSMATRAYKVDGG
                     YRLKGNKMWITNSPVADVFVVWAKEVEPSGTVGQIRGFILDKGMKGLSAPAIHGKVGL
                     RASITGEIVMDDVFVPEENAFPEVRGLKGPFTCLNSARFGIAWGAMGAAEACWHTARQ
                     YTLDRKQFGRPLAANQLIQKKLADMQTDIALGLQAALRVGRMKDEHQNVIEVTSLIKR
                     NNCGKALDIARLARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDVHALILGRAQTG
                     IAAFAN"
     gene            3684..4652
                     /locus_tag="PK518_13250"
     CDS             3684..4652
                     /locus_tag="PK518_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugE"
                     /protein_id="HRM49921.1"
                     /translation="MIRRSILTLGILAMMGSTAQAQSSYPAKPIRLLVPFAAGGTTDL
                     IARVLAEPLGRELGQPVVVENKGGGGGAIGAAETARATPDGYNLGMATVSTTATNPAI
                     NPKIPYNVFTDFTPVVNVAATPNVIAANPQFAGKDYKSFLAEVKKNPGKYSFGSPGTG
                     SIGHLMMEMFMLETGAQMIHVPYRGSGPALNDAVAGQIPLTFDNLPSALPFIKDKRLT
                     ALVVAAPQRLAVLPDVPTLAEVGVPNVNRMAYYGIIGPKGLPKDIVDKVNAAVHKVLK
                     DPAVRKRIEDTGSLIVADTPEKFAQEIKAEYTAYKTVVDKQKLTME"
     gene            4846..5391
                     /locus_tag="PK518_13255"
     CDS             4846..5391
                     /locus_tag="PK518_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49922.1"
                     /translation="MSLMHHPLLRIASAGVLGALMAGCVGVPGDPYYDGGYSGGGYYP
                     GSASVTVYEQPGVIYTPPPVGWRNYPPPPHGWREDAWRERQWREHREREARERREHER
                     RDMERRRDFERRDAERQREAMRHEAERRRELERQRHMEPHAHQPRVPQRPNMDAEAGR
                     HPREREPHNEREHRNPRYDWR"
     gene            5395..6042
                     /locus_tag="PK518_13260"
     CDS             5395..6042
                     /locus_tag="PK518_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRM49923.1"
                     /translation="MPTPSPATPALALLLAAMACWAQPAAAQVMRCTDPATGQVTYTD
                     GGCHRGDAVREVQERQSPQDIARERQQAAEALERRQERLRLEAEQRRDAQQAERAARQ
                     ATTAPRDDPAQSMACRQARQQLQQVLATMGQGMYDEQSRLDAAQRQADLACLSPAEQA
                     RTERRRDHHPGHATPYYAPPVVVVPPRPMPPPPRREIANCNVFRCYDAQGNTYPR"
     gene            complement(6030..>6495)
                     /locus_tag="PK518_13265"
     CDS             complement(6030..>6495)
                     /locus_tag="PK518_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM49924.1"
                     /translation="LLHLGRAGLRLSILRVPGIYAPDRAGGTPEARLRRGTPVLAAPD
                     DVYTNHIHADDLARACVAALWRGQAQRAYNVADDSQLKMGDYFDLAADLYGLPRPPRV
                     PRSQAQEQLPLSLLSFMSESRRLVTTRMARELRLRLRYPTVHTGLLPAGQRG"
BASE COUNT         1178 a         2326 c         2052 g          939 t
ORIGIN      
        1 ttcgctttta gcgcctgccc gggtggtgaa attggtagac gcaggggact caaaatcccc
       61 cgccgcaagg cgtgccggtt cgattccggc cccgggcacc acgaataagg ccgcacagtt
      121 cccagaacgt gcggcttttt ctttgggcca agatgcgaaa aagccccgca agcggggctg
      181 tactgctcag cgtttcatca ccccaggtgg ggcggcgtgt ccgctggctc ggggggtggc
      241 gcgggaacgt cgggctccac gggcggctcc gccggttggc ggtgtatgtg tgcaatgcac
      301 gtcatcatga ccatctgcct cctgacccgc tcattgtgcg cctctccacc atggcagcgg
      361 caaagtgcct ggcacctgcc ccggggctac gccaatcgaa gcccaggccc cgcagatacg
      421 ctgcctgcgg aagatcctgc caaccttgcg gcgcccgcat gcgcaacaag ggccgtattg
      481 cctgcacgcc ggctgagccc ctggcccggc catggtgcag catgaatccg accaccgccg
      541 gcgcaaccat ttcctgcacc tgtgtgcctg caataggcac cattgacagg aatcggctcc
      601 gcccgcaatc accacatccg atcaggacgc agcgtgtgca cattcagtta ctaccgcgag
      661 tggatattaa cattttgcaa cgcatgcgca agtacttctg catctgaccc cagcgcggtc
      721 agttgtcctg gcgccggatc agcttgcgcc ccgtgtcggt gatatgaaaa acccccgtcg
      781 gtgacttgcc gatgtaaccc caatccagca gccgccccga cagatgatgc ttgagcgcat
      841 cgggcaccgc gatggcctcg cccatttcaa gctgcttgag cgtggcgatc tcgtctaccg
      901 tgggttcgaa cgtgggtgcg tggtacatgg gctgagcggt ggccatggcg ttctccttta
      961 tggattggga ttgcgcggcg ccggcaatgt tccacggaca catcacccgc gccacgtagg
     1021 cgacagggta ttgcgagcac gcgcggcgcg caatgggcca atcacccgca ccggatgcaa
     1081 atccacgcga cgaacgcgag cgcgccaacc tgcccaggca agaacccgaa cggctggccg
     1141 cgctgcgcgc cgcctgggag gcctgggacg catccatgcc gccgatacca cccgacgggg
     1201 ctattccgcc aaggacatgc cacagcgctg aaccaacgga ccgccgcacc ccattaggcg
     1261 acaggcccgg ccgccaccat gtcctgcagc cagcgcgcaa acgctgccag tgctggcggc
     1321 tggggctgcg ccgggctgac gaggtagtag ccgcgctcgc cgcgcagcgg ccgggcgcag
     1381 gccacgacca gttcgccgcg cgccatctcg gcctcgatca gcatgggcgg tatcaacgcc
     1441 acgcccaggc cctgggccgc ggccacggcc gtcatcgaga aaagttcata gcgcggcccg
     1501 tccagcgcac gcggcgcgtc ctgcacgccc atggcctcga accactgctg ccagccatag
     1561 ggccgggtgc tttgctgcag cagcggcatg cgcgccagta gctgcggcgc caaaccctgg
     1621 tgcgcgcgcc cggtgccgcg cggcatgccc gactgctctg ccgcctgctc caggaggcgc
     1681 gggctgcaca cgggcagtac atcctcgggc atcagcagct gggcctgcac gccgggccag
     1741 ttggcgacct gctcgggcgt gcccgcgtac agggccgcgt caaagccggt gtcggtgaac
     1801 aggaagggcc gcgtctgggt ctcgatatgc acgacgatct ccgggtggcg cgcggccagc
     1861 tgcggcaggc gcggtatcag ccagcgtgtg gcaaaggtgg ccaccgccgc cagcgagatg
     1921 gcgccgccct ggccctggtg cgccatcaca tccagagtgc cctgctccag cgcctgcagc
     1981 cagcgcgcca cctggcgcgc gtagtggcgc ccggcgggcg taagcaccac gccgtggcgc
     2041 gtgcgccgaa acagctgcac gcccaactga tcctccagcg ccaggatctg gcgcgagacg
     2101 gcgctttgcg tcagcgccag ctcctgtgcg gcgcgggtat agctttcgtg ccgcgcggcg
     2161 gcctcgaaac aggccagggc ctgggtggat ggcagatgtc gtcgcatgcc ggacatctta
     2221 tccatatatt cgtaaacgtc atttcttgat gaaaattact cgcttgccat caacgatagc
     2281 aagggggtac gatcccagcc atcaatcttt catgcctgca atgcataggt tgatcccacc
     2341 cagattcatc atccatcagc actggagaca ccatgacccg cgccgccttc caatgggacg
     2401 acccctttca cctcgaccag cagctcaccg aggacgagcg cgccatccgt gacgccgccc
     2461 acgcctactg ccaggacaag ctggcgccgc gcgtgctgga gatgttccgc cacgagaaga
     2521 ccgacgtctc catcttccgc gagatgggcg agctcggcct gctcggcccc accatcccca
     2581 ccgagtacgg cggcgcgggc ctgaactacg tcagctacgg cctggtggcg cgcgagatcg
     2641 agcgcgtgga ctcgggctac cgctccatgg ccagcgtgca gtcgtcgctg gtgatggtgc
     2701 cgatcaacga attcggctcc gaggcgcaga agcagaaata cctgcccaag ctggcatcgg
     2761 gcgagtacat cggctgcttc ggcctgaccg agcccgacca cggctccgac cccggcagca
     2821 tggccacgcg tgcctataag gtcgatggtg gttatcgcct caagggcaac aagatgtgga
     2881 tcaccaacag ccccgtggcc gacgtgttcg tggtatgggc caaggaagtc gagccatccg
     2941 gcaccgtggg ccagatccgc ggcttcatcc tcgacaaggg catgaagggc ctgtccgccc
     3001 cggccatcca cggcaaggtg ggcctgcgcg cctcgatcac cggcgagatc gtcatggacg
     3061 acgtcttcgt gcccgaggag aacgccttcc ccgaggtgcg cggcctgaag ggcccgttca
     3121 cctgcctgaa cagcgcgcgc ttcggcattg cctggggcgc catgggcgcg gccgaggcct
     3181 gctggcacac cgcgcgccag tacacgctgg accgcaagca gttcggccgg ccgctggccg
     3241 ccaaccagtt gatccagaag aagctggccg acatgcagac cgatatcgcc ctgggcctgc
     3301 aggccgcgct gcgcgtgggc cgcatgaagg acgagcacca gaacgtgatc gaggtcacgt
     3361 cgctgatcaa acgcaacaac tgcggcaagg ccctggatat cgcccgcttg gcgcgcgaca
     3421 tgatgggcgg caacggcatc agcgacgagt tcggcgtggc gcgccacctg gtgaacctgg
     3481 aggtggtcaa cacctacgag ggcacgcacg acgtgcacgc gctcatcctg ggccgtgcac
     3541 agaccggcat cgccgccttt gccaactgag ttaactagca aaaaccatag ctgccttcgc
     3601 ttaatacata agcgctagca gctattttta ttgcaataat ccatcaccaa agcgccagcc
     3661 actcacacaa ggagaccatc cgcatgatcc gtcgcagcat cctcaccctg ggcattctgg
     3721 ccatgatggg cagtaccgct caggcccagt ccagctaccc cgccaagccc atacgcctgc
     3781 ttgtgccctt tgccgccggc ggtaccaccg acctgattgc ccgcgtgctg gccgagccgc
     3841 tgggccgcga actgggccag cccgttgtcg tcgagaacaa gggcggcggc ggcggtgcca
     3901 taggggccgc cgaaaccgcg cgcgccacgc ccgacggcta caacctgggc atggccaccg
     3961 tgtccaccac ggccaccaac ccggccatca acccgaagat cccgtacaac gtgttcaccg
     4021 atttcacgcc cgtcgtgaac gttgcagcca cgcccaacgt gatcgctgcc aacccccagt
     4081 tcgcaggcaa ggactacaag agcttcctcg ccgaggtgaa gaagaatccg ggcaaatact
     4141 cgttcggctc gcccggcacc ggcagcatcg gccatctgat gatggaaatg ttcatgctgg
     4201 agaccggcgc ccagatgatt cacgtgccct accgcggctc cggcccggcg ctcaacgacg
     4261 ccgtggccgg ccagattccg ctgaccttcg acaacctgcc ctccgccctg cccttcatca
     4321 aggacaagcg cctgaccgcc ctggtggtgg ccgcgcccca gcgcctggcc gtgctgccgg
     4381 acgtgcccac actggccgaa gtgggcgtac ccaacgtgaa ccgcatggcc tactacggca
     4441 tcatcggccc caagggcctg cccaaggaca tcgtggacaa ggtgaatgcc gccgtacaca
     4501 aggtgctgaa agaccccgcc gtgcgcaagc gcatcgagga tacgggctcg ctcatcgtcg
     4561 ccgacacgcc cgagaagttc gcccaggaaa tcaaggccga gtacaccgcc tacaagaccg
     4621 tggtggacaa gcagaagctg accatggagt gatcgcccgc ccatggccac acacagcccc
     4681 gccgcccgcg gggctttttg ttgcctgccc ggcatcggaa acgcacagac accatcgtga
     4741 acaatgcgca aagtacggca aaccattgcc gcctgggtca accaaaatac cggctgatgc
     4801 gtctcacacc cagtcaccct gtgctcggca cgattggatc cacacatgtc acttatgcac
     4861 caccccttgt tgcgcatcgc cagcgccggc gtactcggcg cgctgatggc cggctgcgtg
     4921 ggcgtgccgg gtgaccccta ctacgacggt ggctacagcg gcggcggcta ctacccggga
     4981 tcggcatcgg tcacggtgta cgaacaaccc ggcgtgatct acacgccccc gcccgtgggc
     5041 tggcgcaact acccgccgcc gccgcacggc tggcgcgagg acgcctggcg cgaacgccag
     5101 tggcgcgaac accgcgagcg cgaggcgcgt gagcgccgtg agcacgaacg ccgcgacatg
     5161 gagcgccgcc gcgatttcga gcgccgcgac gccgagcgcc aacgcgaggc catgcgccac
     5221 gaggccgagc gccgccggga gctggagcgc cagcgccaca tggagccgca tgcccaccag
     5281 ccgcgcgtgc cgcaacgccc gaacatggat gccgaggccg gccgccaccc acgcgagcgt
     5341 gaaccgcaca acgagcgcga gcatcgcaac ccgcgctacg actggcgcta gtacatgccc
     5401 acaccatccc ctgctacccc tgccctggcc ctgctgctgg cggccatggc gtgctgggcg
     5461 cagcctgcgg cggcccaggt catgcgctgc accgacccgg ccacgggcca ggtgacctac
     5521 accgacggcg gctgccaccg cggcgatgcg gtgcgcgagg tgcaggagcg ccagtccccg
     5581 caggacatcg cgcgcgagcg ccagcaggcg gccgaggcac tggaacgcag gcaggagcgc
     5641 ctgcggctgg aggccgaaca acggcgcgat gcacagcagg ccgaacgcgc agcacgccag
     5701 gccaccaccg ccccccgcga cgacccggcc caatccatgg catgccggca agcgcgccag
     5761 cagctgcagc aggtgctggc caccatgggc cagggcatgt atgacgagca gtcgcggctg
     5821 gacgccgccc agcgccaggc ggatctggcc tgcctgagcc cggccgagca ggcgcgcacc
     5881 gagcgccgcc gcgaccacca tcccggccac gccacgccct actacgcccc gccggtggtg
     5941 gttgtgccac cccgccccat gccgccaccg ccacggcgcg agatcgccaa ctgcaacgtg
     6001 tttcgctgct acgacgccca gggcaatacc tacccacgct gacccgcagg cagcagccct
     6061 gtatgcaccg tggggtagcg caggcgcagg cgcagttcgc gcgccatgcg cgtggtcacc
     6121 agccggcgcg actcgctcat gaagctcagc aacgacagcg gcagctgctc ctgcgcctgg
     6181 ctgcgcggca cacgcggcgg gcgcggcagg ccgtacaggt cggccgccag gtcaaagtag
     6241 tcgcccatct tcagctggct gtcatcggcc acgttgtagg cgcgctgcgc ctgcccacgc
     6301 cacagcgcgg ccacgcaggc acgcgccagg tcgtcggcat ggatatggtt ggtatagaca
     6361 tcgtcgggcg cggccagcac cggggtgccg cgacgcagcc gcgcctcggg cgtgccgccc
     6421 gcgcgatcgg gcgcatagat gccgggcacg cgcaggatgc tcaggcgcag gccggcgcgc
     6481 cccaggtgga gcagc
//
LOCUS       DAOMTQ010000164         6492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2764_length_6492_cov_26.437005, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000164 DAOMTQ010000000
VERSION     DAOMTQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2764_length_6492_cov_26.437005"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PK518_13270"
     CDS             complement(<1..773)
                     /locus_tag="PK518_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRM49925.1"
                     /translation="MFVTPQSPLPAARPRALALAPLLALALAGCAATGPSEPLGSPVV
                     VPTAWANRPATQAAPGPDNLAQWWQQLGDAQLTGLIDEALQAHTSMRSARAAVEQARA
                     QRDMQAAGLLPSVDGSASAQRSRSSGNTANSFQAGLDASWEPDLFGRIGSGVRAGDAD
                     LLAAEAGLAAARVALAAEVAVNYIDLRGQQQRLAIAERNLAAQRETAQITQWRVQAGL
                     ANSLAEQQARTSVEQTAAQLPALRSAMAQSRHALAVLTGR"
     gene            complement(848..1888)
                     /gene="tsaD"
                     /locus_tag="PK518_13275"
     CDS             complement(848..1888)
                     /gene="tsaD"
                     /locus_tag="PK518_13275"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRM49926.1"
                     /translation="MSQLILGIESSCDETGVALVRAEGGGVPRLLAHALHSQIDMHQA
                     YGGVVPELASRDHIRRVLPLTQTVLHEAGEHLDAVDVVAYTRGPGLAGALLVGAGVAC
                     ALGAALDIPVLGVHHLEGHLLSPFLSSDPPEFPFIALLVSGGHTQLMRVDGVGRYEIL
                     GETIDDAAGEAFDKSAKLMGLGYPGGPALSRLAEQGDAKAFKLPRPLLHSGDLDFSFA
                     GLKTAVLTQAKKLGDELVTRKADLAASTQAAIVEVLVKKTLAALDQSGMRRVVVAGGV
                     GANRLLRAQLDAACAARGVRVHYPELELCTDNGAMIAMAAAMRLQAGRQQANRDYAFD
                     VKPRWPLDAIAT"
     gene            complement(1944..2381)
                     /locus_tag="PK518_13280"
     CDS             complement(1944..2381)
                     /locus_tag="PK518_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM49927.1"
                     /translation="MTTVAEIIKSKPDGKVHSVKPTDTVLSALKLMADMHIGALLVME
                     GDKIAGIFTERDYARKVVLLGRASIDTPISEVMTRAVRFVHPTNSAEECMALMTENRL
                     RHLPVMDGEQVVGLVSIGDLVKSVISEQQFIITQMEQYITRVW"
     gene            complement(2470..3135)
                     /locus_tag="PK518_13285"
     CDS             complement(2470..3135)
                     /locus_tag="PK518_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRM49928.1"
                     /translation="MNTPASTPLLAQELMQHLQGAPLQQMAQQLGSDQAQTQSAVEMA
                     LPLLLGALGRNAQDEQGAQSLFGALQRDHMDAASQQALGLGGMDLGGLLGSVLGAGAP
                     SGAAMGGGAGAAILGHIFGGGRERAESGLGQASGLGANAGQLMQMLAPIVMSFLAQRV
                     SSGGMDAGGLGQMLGQERASAQRQGAPGGDLLSSLLDQDGDGQLGLGDLLKLGGSLLG
                     GRR"
     gene            3421..4719
                     /locus_tag="PK518_13290"
     CDS             3421..4719
                     /locus_tag="PK518_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM49929.1"
                     /translation="MTTATRDHRPTALVFPHPLRRHGGRLLGSLSLALALAACGDKAA
                     SPVAQQGPAQVGVITLQTQQQQLDATLPGRTRASLTAEVRPQVSGIVQQRLFTEGALV
                     RKGQALYQIDDRALRAAEASAEAALARAEASARTLEATARRNAELLKIDAISHQAHDE
                     SQAAAAQARADMGVARANLETARINLRYSRIEAPIAGRISLSSVTPGALVTANQTNAL
                     TTIVQMDPMYVDFTQSSNEIVQLRRDWDAGRYQKLDGNQARVRIRLDDGSDYAHEGRL
                     QFAGMIVNDSTGTVTLRAVVPNPEGVLMPGMYVQALLPTGLASDALLIPQQAVNRDLT
                     GRANVLVVNADDVVEKRPVELARALGSRWLLDSGLVAGERLVVDGFQRVKPGDKVSPQ
                     PVVLKTSKGEAGPPQAPEDGLRPRNGQGVNIATPQAGAPR"
     gene            4733..>6492
                     /locus_tag="PK518_13295"
     CDS             4733..>6492
                     /locus_tag="PK518_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM49930.1"
                     /translation="MAQFFINRPIFAWVLSIIVMLAGGLAIFTLPLEQYPDIAPPRVT
                     ISAQYTGASAETVENSVTQIIEQQLKGIDNMIYMSSTSDASGRSATTLTFAPGTNIDV
                     AQVQVQNKLQSAMNRLPEAVKSRGVFVNKGGQDYLVTYSFFSGDPKVTAVDVGDYLNS
                     NLVDVIGRLDGVGDIRVFGTFYAMRIWMDPARMEKYQLMPSDLIAALNAQNAQVSAGQ
                     LGALPAVPDQQLNATITARTKLQTVPEFEAIVLKTATDGAVVTIKDVARVELGPDTLS
                     IKAKLNGRPGAGMGIVLADGANAMAVSDAVAAKLAELKPFFPNEIDYFVSSDSTPFVR
                     ASIKEVVITLAEAMVLVVLVMFVFLQNLRATLIPAIAVPVVLLGTFGVLAAAGYSINT
                     LTMFALVLAIGLLVDDAIVVVENVERVMHDTGLPPKEATRHSMREITPALVGIGVTLA
                     AVFVPMAFFGGSTGVIYRQFSITIVAAMALSVFVALTLTPALCASILKAPTHGGGAPR
                     IRTGLLGLSDRFFLWFNRQFDAGAARYQGRVAWMLHRAKRMLLIFAAICLAVWWIITR
                     VPTSFLPEEDQGYILASINLP"
BASE COUNT         1068 a         2293 c         2153 g          978 t
ORIGIN      
        1 cgaccggtga gcacggccag cgcatggcgc gactgggcca tggccgagcg cagcgcgggc
       61 agctgggcgg ccgtctgctc gacgctggtg cgcgcctgct gctcggccag cgaattcgcc
      121 aggcccgcct gcacgcgcca ttgggtgatc tgcgccgtct cgcgctgcgc ggccaggttg
      181 cgctcggcaa tcgccaggcg ctgctgctgg ccgcgcaggt cgatgtagtt caccgccacc
      241 tcggccgcca gcgccacccg ggccgcggcc aggccggcct cggcggccag caggtcggcg
      301 tcgccagcgc gtacgccact gccaatgcgg ccaaacaggt cgggctccca gctcgcgtcc
      361 agccccgcct ggaagctgtt ggctgtattg ccgctgctgc ggctgcgctg ggccgaagcg
      421 gacccatcca ccgacggcag caggcccgcg gcctgcatgt cgcgctgggc gcgcgcctgc
      481 tccacggcgg cgcgcgcgct gcgcatgctg gtgtgggcct gcagcgcctc gtcgatcagg
      541 ccagtgagct gggcgtcgcc cagttgctgc caccactggg ccaggttgtc tggcccgggc
      601 gcagcctgcg tggctggccg attcgcccag gctgtgggta cgaccacagg gctgcccagc
      661 ggctcggatg gacccgtagc ggcgcagccg gccagcgcca aggccagcag cggcgccaga
      721 gccaacgctc gcggccgggc tgccgggagg ggagattgcg gcgtaacaaa catgccggca
      781 ttgttaccgc gccatttgta gcgcccgcca gcggcgcatg aacttttgta aagacgcagc
      841 gcaaccttca ggtagctatg gcatccagcg gccagcgcgg cttgacgtca aaggcgtagt
      901 cacggttggc ctgctgacgg ccagcctgca ggcgcatggc ggcggccatc gcgatcatgg
      961 cgccgttgtc ggtgcacagt tccagctcgg ggtaatgcac gcgcacgccg cgtgccgcgc
     1021 aggcggcgtc cagctgcgcg cgcagcagcc ggttggcgcc cacgccgccg gccaccacca
     1081 cgcggcgcat gccgctctgg tccagggccg ccagggtctt cttgaccagc acctcgacga
     1141 tggcggcctg ggtgctggcg gccaggtcgg ccttgcgcgt caccagctcg tcgcccagct
     1201 ttttggcctg ggtcagcacc gccgtcttca ggccggcaaa ggaaaagtcc aggtcgccgc
     1261 tgtgcagcag cgggcgcggc agcttgaagg ccttggcgtc gccctgctcg gccaggcgcg
     1321 acagggcggg accaccgggg tagcccaggc ccatgagctt ggcggacttg tcgaaggcct
     1381 cgccggccgc gtcgtctatg gtttcgccca gaatttcgta gcggccgacg ccgtccacgc
     1441 gcatcagctg ggtgtggccg ccggagacca gcagggcgat gaagggaaac tcgggcgggt
     1501 cgctgctcaa aaacggcgac agcagatggc cttccaggtg atgcaccccc agcaccggaa
     1561 tgtccagcgc cgcgcccagc gcacaggcca cgccggcgcc caccagcagc gcgcccgcca
     1621 ggccggggcc gcgcgtgtag gccaccacat ccacggcgtc cagatgttca ccagcttcgt
     1681 gcaacacggt ttgcgtcagc ggcagcacgc ggcggatatg gtcgcggctg gcaagctccg
     1741 gcaccacgcc gccataggcc tggtgcatgt cgatctggct gtgcagggca tgggccagca
     1801 gccgcggcac gccgccgccc tcggcacgca ccagggccac gccggtttcg tcacaggaag
     1861 attcgatgcc caggatcagc tgactcatgc ccgggagtgt agtggcgccc ctcagcactt
     1921 gggctgcggg ggcttatgcg gcatcaccag acgcgggtga tgtattgctc catctgcgtg
     1981 atgatgaact gctgctcgga gatcacgctc ttgaccaggt cgccgatgga caccaggccc
     2041 accacctgct cgccgtccat caccggcagg tggcgcaggc ggttttcggt catcagcgcc
     2101 atgcattcct cggcgctgtt ggtcgggtgc acgaagcgca cggcgcgcgt catgacctcg
     2161 ctgatcgggg tgtcgatgga ggcccgcccc agcagcacca ccttgcgggc atagtcgcgc
     2221 tcggtgaaga tgccggcgat cttgtcgccc tccatcacca gcagcgcacc gatgtgcatg
     2281 tcggccatga gcttcaaggc ggagagcacg gtgtccgtcg gcttgaccga atgcaccttg
     2341 ccgtcgggtt tggacttgat gatttctgca acagttgtca aagcggcctc ccaggctgtc
     2401 aagtgaaacg agggcctgag tttccgccga accggggcct gaatgcaacc cggtacctgt
     2461 cagcgtgtat cagcgccgcc cgcccaacag actaccacca agtttgagca agtcacccaa
     2521 tcccagctgg ccgtcgccgt cctgatcgag caggctgctc agcaggtcgc caccgggcgc
     2581 gccctggcgc tgggcgctgg cacgctcctg accgagcatc tgccccaggc cgccggcgtc
     2641 catgccgccc gagctcacgc gctgggccag aaaggacatg acgatgggcg ccagcatttg
     2701 catcagctgg ccggcgtttg cccccaggcc gctggcctgg cccagcccgg attcggcacg
     2761 ctcgcgtcca ccgccaaaaa tatgccccag gatggcagcg ccggcaccac cgcccatcgc
     2821 agcacccgac ggcgcgccag caccgagcac ggagccaagc aggccgccca gatccatgcc
     2881 gcccaggccc agtgcctgct gcgacgccgc atccatgtgg tcgcgctgca aggcgccgaa
     2941 cagcgactgc gcgccctgct cgtcctgggc gttgcggccc agggcgccga gcaacagcgg
     3001 caaggccatt tccaccgcgc tttgcgtctg tgcctggtcg gaacccagtt gctgggccat
     3061 ctgctgcagg ggcgcgccct gcaggtgctg catcaactct tgggccaaca ggggggtgga
     3121 tgcgggggtg ttcatgacag gttgctccta tgcaaggtca atgggatcag cgacagcgac
     3181 ccaaccgtgc atggtatgcg ctacccgaaa ccggacatgc ggcaaggccc ggccaagcaa
     3241 ccaagtttga caattgcgcg cggcaggggc gagtaaagaa aaatgaagta acgcagccag
     3301 cgcaacaggc ccggcggcga tatgcgacag tgcgacgttt accttcggtg acattcaacc
     3361 gaggcttgcc acaccacgaa gacaatgatg gcttcgccag gactattgcg ctgcgccccc
     3421 atgactaccg cgacccgcga ccaccgcccc accgccctcg tatttccgca cccgctgcgc
     3481 cgccacggcg gacggctgct gggatcactg agcctggccc tggccctggc ggcctgtggt
     3541 gacaaggcgg caagcccggt ggcccagcag gggccggccc aggtcggcgt gatcacgctg
     3601 cagacgcagc agcagcagct tgacgccacc ctgcccgggc gcacccgcgc ctcgctcacg
     3661 gccgaggtgc ggccacaggt ctcgggcatc gtgcagcagc gcctgttcac cgaaggcgcc
     3721 ctggtgcgca agggccaggc gctgtaccag atcgacgacc gcgcgctgcg cgccgccgag
     3781 gccagcgccg aggccgccct ggccagggcc gaggccagcg cacgcacgct ggaggccacg
     3841 gcacggcgca acgccgagct gttgaagata gacgccatca gccaccaggc ccatgacgag
     3901 agccaggccg cggccgcgca ggcgcgcgcc gacatgggcg tggcccgcgc caacctggag
     3961 acggcacgca tcaacctgcg ctacagccgc atcgaggcgc cgatcgccgg gcgcatcagc
     4021 ctgtccagcg tcacgcccgg cgccctggtc acggccaacc agaccaacgc cctgacaacc
     4081 atagtgcaga tggatccgat gtacgtggac ttcacccagt ccagcaacga gatcgtgcag
     4141 ctgcgccgcg actgggacgc gggccgctac cagaagctgg acggcaacca ggcgcgcgtg
     4201 cgcatacgcc tggacgacgg cagcgactac gcgcacgagg gccggctgca attcgccggc
     4261 atgatcgtca acgacagcac gggcacggtg acgctgcgcg ccgtggtgcc caaccccgag
     4321 ggcgtgctga tgcccggcat gtatgtgcag gcactgctgc ccacgggcct ggcgagcgac
     4381 gccctgttga taccgcagca ggccgtgaac cgcgacctga cgggccgcgc caatgtgctg
     4441 gtggtgaacg ccgacgacgt ggtcgaaaag cgccccgtgg agctggcgcg cgccctgggc
     4501 agccgctggc tgctggacag cggcctggtc gccggcgagc gcctggtggt ggacggcttt
     4561 caacgcgtca agcccggcga caaggtcagc ccccaaccgg tggtcctgaa gaccagcaag
     4621 ggcgaggccg gcccgcccca ggcccccgag gacggcttgc gccccagaaa tggccagggc
     4681 gtgaacatcg ccacgccgca ggccggcgcc cctcgataag cggcaggcgc acatggccca
     4741 gttcttcatc aaccgcccca tcttcgcgtg ggtgctgtcc atcatcgtca tgctcgccgg
     4801 cggcctggcg atcttcacgc tgccgctgga gcaatacccc gacatagcgc cgccgcgcgt
     4861 caccatctcg gcgcagtaca cgggggcatc ggccgagacg gtggaaaact cggtgacgca
     4921 aatcatcgag cagcagctca agggcataga caacatgatc tacatgagct cgacgtcgga
     4981 cgcgtcgggc cgatcggcca ccaccctcac ctttgcgcca ggcaccaaca tcgacgtggc
     5041 ccaggtgcag gtgcagaaca agctgcagtc ggccatgaac cgcctgcccg aggccgtcaa
     5101 gagccgcggc gtgttcgtca acaagggcgg ccaggactac ctggtgacct acagcttctt
     5161 ctcgggcgac cccaaggtca cggccgtgga tgtgggcgac tacctgaact ccaacctggt
     5221 ggacgtgatc ggccgcctgg acggcgtggg cgacatccgc gtgttcggca ccttctacgc
     5281 catgcgtatc tggatggatc cggcgcgcat ggagaagtac cagctcatgc cctcggacct
     5341 gatcgccgcg ctgaacgcgc agaacgcgca ggtctcggcc ggccagctgg gcgccctgcc
     5401 ggccgtgccg gatcagcagc tcaacgccac catcaccgcg cgcaccaagc tgcagaccgt
     5461 gcctgaattt gaagccatag tactcaaaac cgccacggat ggtgcggtgg taactatcaa
     5521 agatgtagca cgcgtggagc tgggcccgga taccctgtcg atcaaggcca agctcaacgg
     5581 ccggcccggc gccggcatgg gcatcgtgct ggccgacggc gccaacgcca tggccgtgtc
     5641 cgacgccgtg gcggccaagc tggccgagct caagcccttc ttccccaacg agatcgacta
     5701 cttcgtcagc tccgactcca cgcccttcgt gcgcgcctcc atcaaggagg tggtcatcac
     5761 cctggccgag gccatggtgc tggtggtgct ggtgatgttc gtctttctgc agaacctgcg
     5821 cgccacgctg atcccggcga tcgccgtgcc ggtggtgctg ctgggcacct ttggcgtgct
     5881 ggcggccgcg ggctattcca tcaacaccct gaccatgttc gccctggtac tggccattgg
     5941 cctgttggtg gacgacgcca tcgtggtggt ggagaacgta gagcgcgtga tgcacgacac
     6001 cggcctgccg ccgaaggagg ccacgcgcca ctccatgcgc gagatcacgc ccgcgctggt
     6061 gggcattggc gtcacgctgg ccgccgtgtt cgtgcccatg gccttctttg gcggctccac
     6121 gggcgtgatc taccggcagt tctccatcac catcgtggcg gccatggcgc tgtcggtgtt
     6181 cgtggcgctc acgctcacgc cggcgctgtg cgccagcatc ctcaaggcgc ccacgcacgg
     6241 gggcggcgcg ccccgcatcc gcaccggtct gctgggcctg agcgatcgct tctttctctg
     6301 gttcaaccgc cagttcgacg ccggggccgc ccgctaccag ggccgtgtgg cctggatgct
     6361 gcaccgcgcc aaacgcatgc tgctgatctt tgccgccatc tgcctggccg tgtggtggat
     6421 catcacccgc gtgccgacct ccttcctgcc cgaggaagac cagggctaca tcctggccag
     6481 catcaacctg cc
//
LOCUS       DAOMTQ010000165         6472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2783_length_6472_cov_22.471716, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000165 DAOMTQ010000000
VERSION     DAOMTQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_6472_cov_22.471716"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PK518_13300"
     CDS             <1..445
                     /locus_tag="PK518_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HRM49931.1"
                     /translation="LAAGLPAEVALRSRPGQPLAARVLRTEPLADAVTEELLAKIVFD
                     ALPEPLPPIGELAELTVRLPALPESPTIPNAALRTVAGQRGVWKITDGALAFAPVRLG
                     RADLDGQVQVLDGLAVGDSIVLYSEKALTAKSRVHVVERIAGVAP"
     gene            442..1641
                     /locus_tag="PK518_13305"
     CDS             442..1641
                     /locus_tag="PK518_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49932.1"
                     /translation="MISLAGRDILHTWGKFVFTGIGLGLLIGVTLTMAGVYRGMVEDG
                     RVLLDNSGADLWVVQRDTLGPYAESSSVPDDLWRGIRAMPGVVQAANITYLTMQVGLG
                     EQDVRAMVVGISPGGSGEPGWPPQLVAGRQITRGHYEAVADLASGARLGDVLRIRRNH
                     YRVVGLTRRMVSSSGDPMVFIPLKDAQEAQFLKDNDAILMQRRRTEANPAFNRPAVPD
                     LLDAVIASQTSNSYVNAVLVRIAPEFTAQQVAEPIRRWKRLTVYDRAQMEGILVGKLI
                     ATSARQIGMFLVILALVSAAIVAFLIYTLTMDKIREIAVLKLIGTKNRTIAAMIVQQA
                     LVLGLIGFVVGKITATFAAPLFPKYVLLVPFDTAAGFAAVLAICVLASVVAIRVALRV
                     DPAEAIG"
     gene            1646..2356
                     /locus_tag="PK518_13310"
     CDS             1646..2356
                     /locus_tag="PK518_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM49933.1"
                     /translation="MNRQGIRIERLSKRYGSGDTAFWALKDVNMQVAPGEVVGLIGPS
                     GSGKSTLLKCLGAVIDPTSGRMTLDDQVIYDEGWRVRDLGALRRDQIGFVFQAPYLIP
                     FLDATDNVALLPMLAGVPNDEARRRALELLTALDVQHRARAMPSQLSGGEQQRVAIAR
                     GLVNRPPVILADEPTAPLDSQRAMAVIRILNDMAQQLQTAVIVVTHDEKIIPTFKRIY
                     HIRDGVTHEEAGEGRGLG"
     gene            complement(2440..3747)
                     /locus_tag="PK518_13315"
     CDS             complement(2440..3747)
                     /locus_tag="PK518_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRM49934.1"
                     /translation="MRFLFLPAAISLALITGCASTTAPNQPSASAPAAVPAPPTPPVV
                     PTPDATPATAGTSDLARTIAGFHAWLRSFAQEARAAGISQQTVDATLAQARWQPRVVE
                     LDRAQPEFTRTPWAYLDSAVSPQRVAQGREQRRLHAAALDAAEQRHGVPAGIITAIWG
                     MESNYGGNFGSFSAVDALATLAYDGRRRDWARGELLAALRIVDRGDIAADALVGSWAG
                     AMGHTQFLPSVYLAHAVDADGDGRRDIWGSVPDVLASTANFLAHSGWQAGQPWGVEVR
                     LPPGFDYARTELNVRQDSQAWALDGVRAVDGQPLPALPNAAIVTPAGASGPAILVGPN
                     FGALLRYNNSVNYALAVGLLARQIDGGDGLLTPWPRDLPALSRGEIKVLQQALNDRGF
                     GAGAVDGVLGPATRAGVRRLQQSLGLPADGFATPQLLERLQAP"
     gene            3816..5252
                     /gene="sbcB"
                     /locus_tag="PK518_13320"
     CDS             3816..5252
                     /gene="sbcB"
                     /locus_tag="PK518_13320"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HRM49935.1"
                     /translation="MHTFVWHDYETFGTDTRRDRPAQFAAIRTDADLNEIGTPLMIYC
                     RPANDYLPDPQSCLITGITPQLCLQKGLPEHEFAERIEAELALPGTIGVGYNTIRFDD
                     EITRHMFWRNLIDPYAREWQNQCGRWDLLDVVRMCYALRPDGIHWPMVDGKPSFRLEH
                     LTKANGLAHEAAHDALSDVRATIALARLVRQHNPKLFDFALSLHKKERVAAELRLPAT
                     AQTARPFLHVSGMFPAERGCLAVMWPLASHPTNKNELLAWDLAQDPAELATLQPDEIR
                     QRLFTRQADLPEGVTRLPLKSVHLNKSPMVVGNLNTLSPAMAERWRMDLAQAARHAQA
                     ARELPDMSAIWAEVFQRPREATPDVEQDLYGGFVGNEDRRRLNRLRALPPEELARDGT
                     GFDDGRLAELVWRYRARNFPQTLTPEDHERWEAHRQARLVEGEGGGLSFDALFERLDQ
                     LGAEADERGQQILEAVYDYAESIAPGME"
     gene            5221..6123
                     /locus_tag="PK518_13325"
     CDS             5221..6123
                     /locus_tag="PK518_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HRM49936.1"
                     /translation="MPKASPPAWSEGGAPLQALHAFVQRHPRLFVITGAGCSTAAGIP
                     DYRDANGDWKRPQPVNYQAFMGDAATRRRYWARSLLGWPVMAGARPGAAHQALAALEG
                     AGRVELLLTQNVDGLHHAAGSQRTLDLHGRIDTVRCMHCHARSPRAALQAELLRRNPA
                     WAALRAGAAPDGDADLEGRDFSGFDVPDCAQCGTGLLKPDVVFFGENVPRERVEAAHA
                     ALARADAVLVAGSSLMVYSGFRFVQAAVDRGLPVVAINQGRTRADGLLALKLQQDVGQ
                     TLVDLAHQLGVAVSRWQSPVISGS"
     gene            6256..6354
                     /gene="kdpF"
                     /locus_tag="PK518_13330"
     CDS             6256..6354
                     /gene="kdpF"
                     /locus_tag="PK518_13330"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HRM49937.1"
                     /translation="MTGLTLIHALAGALALGLFVYLGYALLRPENF"
BASE COUNT          984 a         2188 c         2290 g         1010 t
ORIGIN      
        1 cctggcggcg ggcctgccgg ccgaggtggc gctgcgctcg cgccccggcc agccgctggc
       61 cgcgcgcgtg ctgcgcaccg aaccgttggc cgatgccgtg accgaggagc tgttggcaaa
      121 aatcgtcttt gatgcgctgc ccgagccgct gccgccgatt ggcgagctgg ccgagctcac
      181 cgtgcgcctg ccggccctgc ccgaatcgcc caccatcccc aatgccgccc tgcgcaccgt
      241 ggccggccag cgcggcgtgt ggaagatcac tgatggcgcg ctggcctttg cccccgtgcg
      301 cctgggccgc gccgacctgg acggccaggt gcaggtgctg gacggcctgg ccgtgggcga
      361 ttccatcgtg ctctacagcg aaaaggccct caccgccaag agccgcgtgc atgtggtcga
      421 gcgcattgcc ggggtggcgc catgatcagc ctggccgggc gcgacatcct gcacacctgg
      481 ggcaagttcg tcttcaccgg cataggcctg ggcctcttga tcggcgtgac gctgaccatg
      541 gccggcgtct accgcggcat ggtggaagac ggccgggtac tgctggacaa cagcggcgcc
      601 gacctgtggg tggtgcaaag ggataccctg ggcccgtatg ccgaatcgtc cagcgtgccc
      661 gacgacctgt ggcgtggcat acgcgccatg cccggcgtgg tgcaggcggc caacatcacc
      721 tacctgacca tgcaggtggg cctgggcgag caggacgtgc gcgccatggt ggtgggcatc
      781 agccccggcg gctccggcga gcccggctgg ccgccgcagc tggtggccgg gcggcagatc
      841 acgcgcggcc attacgaggc cgtggccgac ctggccagcg gcgcgcgcct gggcgacgtg
      901 ctgaggatcc gccgcaacca ttaccgggtg gtggggctca cgcggcgcat ggtgtcctcc
      961 agcggcgacc cgatggtgtt cataccgctg aaggacgcac aggaggcgca gtttctgaag
     1021 gacaacgacg ccatcctgat gcagcgccgc cgcaccgagg ccaacccggc cttcaaccgc
     1081 ccggccgtgc cggacttgct ggacgcggtg attgcctcgc agaccagcaa cagctatgtc
     1141 aacgccgtgc tggtgcgcat agcgcccgaa ttcacggcgc agcaggtggc cgagcctatc
     1201 cgtcgctgga agcgcctcac cgtgtacgac cgcgcgcaga tggagggcat tctggtgggc
     1261 aagctgatcg ctacctcggc ccggcagatc ggcatgtttc tggtgatatt ggcgctggtc
     1321 agcgcggcca tcgtggcgtt tctgatctac acgctgacca tggacaagat ccgcgagatc
     1381 gccgtgctca agctgatcgg cacgaagaac cgcaccattg ccgcgatgat cgtgcagcag
     1441 gcgctggtgc tgggcctgat cggttttgtc gtgggcaaga tcacggccac ctttgctgcg
     1501 ccgctgttcc ccaaatatgt gctgctggtg ccgttcgaca cggccgcggg ctttgccgct
     1561 gtgctggcga tctgcgtgct ggccagcgtg gtggcgatac gcgtggcgct gcgggttgac
     1621 cctgccgagg ccatagggtg aggccatgaa ccggcagggc atacgcatag agcgcctgag
     1681 caagcgctat ggctcgggcg acacggcatt ttgggcgctc aaggacgtga acatgcaggt
     1741 cgcgcccggc gaggtggtgg ggctgatcgg cccctcgggc tcgggcaaga gcacgctgct
     1801 caaatgcctg ggcgcggtca tagaccccac atcggggcgc atgacgctgg acgaccaggt
     1861 gatctacgac gagggctggc gggtacgcga cctgggcgcg ttgcgccgcg accagatcgg
     1921 cttcgtgttc caggcgccct acctgatccc gttcctggac gccaccgaca acgtggccct
     1981 gctgcccatg ctggccggcg tgcccaacga cgaggcgcgc cggcgcgcgc tggagctgct
     2041 caccgcgttg gacgtgcagc accgcgcgcg cgccatgccg tcgcagctgt cgggcggcga
     2101 gcagcagcgc gtggccatcg cgcgcggcct ggtcaatcgc cccccggtca tcctggccga
     2161 tgagcccacg gcgccgctgg attcacagcg cgccatggcg gtgatccgca tcctgaacga
     2221 catggcacag cagctgcaga cggccgtgat cgtggtcacg cacgatgaaa aaatcatccc
     2281 caccttcaag cgcatttacc acatacgcga cggcgtcacc catgaggagg cgggcgaggg
     2341 acgcggcctt ggatgatgtt ttcttgaaag aaatatgcct ctggcgcttt atggacaagc
     2401 gcaagcagct atcaaaaaaa tagtattttc agttgctgct cacggcgcct gcaggcgttc
     2461 gagcagctgc ggcgtggcaa agccatcggc cggcaggccc aggctttgct gcaggcggcg
     2521 cacgcccgcg cgcgtggccg ggcccagcac gccgtcgacc gcgccggcgc caaaaccgcg
     2581 gtcgttcaat gcctgctgca gcaccttgat ctcgccgcgc gagagcgcgg gcaggtcgcg
     2641 cggccagggc gtgagcaggc cgtccccgcc gtcgatctgg cgcgccagca ggcccacggc
     2701 cagcgcgtag ttgaccgagt tgttgtagcg cagcagggcg ccaaagttgg gccccaccag
     2761 gatggccggg ccgctggcgc cggcgggcgt gacgatggcg gcattgggca gcgcgggcag
     2821 cggctggccg tccacggcgc gcacgccatc aagagcccag gcctggctgt cctggcgcac
     2881 gttcagctcg gtgcgcgcgt agtcaaagcc cggtggcagg cgcacctcca caccccaggg
     2941 ctggcctgcc tgccagcccg agtgcgccag aaagttggcc gtggaggcca gcacatccgg
     3001 cacgctgccc cagatgtcgc gccgcccgtc gccgtcggca tccaccgcat gggccaggta
     3061 caccgagggc aggaactgcg tgtggcccat ggcgccggcc caggagccga cgagcgcatc
     3121 ggccgcgatg tcgccccggt ccacgatgcg cagggcggcc agcagttcgc cgcgtgccca
     3181 gtcgcggcgc cggccgtcat aggccagcgt ggccagcgca tccacggccg agaagctgcc
     3241 gaagttgccg ccgtagttgc tctccatgcc ccagatggcc gtgatgatgc cggccggcac
     3301 gccgtggcgc tgctcggcgg cgtccagcgc cgcggcatgc aggcggcgct gctcgcgccc
     3361 ctgggccacg cgctggggcg ataccgcgct gtcgaggtag gcccagggcg tgcgcgtgaa
     3421 ttcgggctgg gcgcggtcga gctccaccac gcgcggctgc caccgggcct gggccagcgt
     3481 ggcgtccacg gtctgctggc tgatgccggc ggcccgggcc tcctgggcga agctgcgcag
     3541 ccaggcgtga aaaccggcga tggtgcgcgc caggtcgctg gtgccggcgg tggcgggcgt
     3601 ggcatcaggc gtcggcacga cgggtggcgt cgggggcgcg gggacggcgg cgggggcgga
     3661 agcggagggc tgattggggg ccgtggtgct ggcacagcca gtgatcaacg ccaggctgat
     3721 ggcggcggga agaaacagaa aacgcatacg gcgattgtgc cgccgcgggc catgcatact
     3781 ccgggcattg tttccgcctg caacccccaa gcctcatgca caccttcgtc tggcacgact
     3841 acgaaacctt tggcaccgac acgcgccgcg accgcccggc gcagtttgcc gccattcgca
     3901 ccgatgccga cctgaacgag atcggcacgc cgctgatgat ttactgccgg ccggcgaacg
     3961 actacctgcc cgacccgcag tcgtgcctga tcaccggcat cacgccccag ctgtgcctgc
     4021 aaaagggcct gcccgagcat gagtttgccg agcgcatcga ggccgagctg gctctgcccg
     4081 gcaccatagg cgtgggctac aacaccatcc gttttgacga cgagatcacg cgccacatgt
     4141 tctggcgcaa cctcatcgac ccctatgcgc gcgaatggca gaaccagtgc ggacgctggg
     4201 acttgctgga tgtcgtgcgc atgtgctacg cgctgcgccc ggacggcatc cactggccca
     4261 tggtcgatgg caaacccagc ttcaggctgg agcatctgac gaaggccaac ggcctggcgc
     4321 atgaggccgc gcacgatgcg ctgtccgacg tgcgggcaac gattgctctg gcgcggctcg
     4381 tgcgccagca caaccccaag ttgttcgact ttgccctgtc gctgcacaag aaggagcgcg
     4441 tggccgccga gttgcgcctg ccggccacgg cgcagacggc gcggccgttt ttgcatgtct
     4501 cgggcatgtt cccggccgag cgcggctgcc tggccgtgat gtggccgctg gccagccacc
     4561 cgaccaacaa aaacgagctg ctggcctggg atttggcgca ggacccggcc gagctggcga
     4621 ccttgcagcc cgacgagatc cgccaacgcc tgttcacgcg ccaggccgat ctgcccgagg
     4681 gcgtgacgcg actgcctttg aaaagcgtgc acctgaacaa gtcgcccatg gtggtgggca
     4741 atttgaacac gctgtccccg gccatggccg agcgctggcg catggatctg gcgcaggccg
     4801 cgcgccacgc ccaggccgcg cgcgagctgc ccgacatgag cgccatctgg gccgaggtct
     4861 tccagcggcc gcgcgaggcc acgcccgacg tggagcagga tctgtacggt ggcttcgtgg
     4921 gcaacgagga cagacgccgc ctgaatcgcc tgcgcgcgct gccgcccgag gagctggcgc
     4981 gcgatggcac cggctttgac gatggccgcc tggccgagct ggtctggcgc taccgggcgc
     5041 gcaactttcc gcagaccctg acgcccgaag accatgagcg ctgggaggcg caccgccagg
     5101 cccggctggt ggagggcgag ggcggcggcc tgagctttga tgcgctgttc gagcgcctgg
     5161 accagctcgg tgccgaggcc gacgagcgcg gccagcagat tctggaggcc gtgtacgact
     5221 atgccgaaag catcgccccc ggcatggagt gagggcggcg cgccgctgca ggcgctgcac
     5281 gccttcgtgc aacgccaccc gcgcctgttc gtgatcaccg gcgcgggctg cagcacggcg
     5341 gccggcattc ccgactaccg cgatgccaat ggcgactgga agcgcccgca gcccgtcaac
     5401 taccaggcct tcatgggcga cgcggccacg cgccggcgct actgggcgcg cagcctgctg
     5461 ggctggccgg tgatggctgg cgcgcggccc ggtgcggcgc accaggcgct ggcggccctt
     5521 gagggcgcgg gccgcgtcga gctgctgctg acccagaacg tggatggcct gcaccatgca
     5581 gcgggcagcc agcgcacgct ggatctgcat ggccgcatcg acaccgtgcg ctgcatgcat
     5641 tgccatgcgc gcagtccacg cgctgccctg caggccgagc tgctgcggcg caaccccgcc
     5701 tgggcggcgc tgcgcgcggg ggccgcgccc gatggcgacg ccgatttgga ggggcgcgac
     5761 ttttccggct tcgacgtgcc agattgcgcg caatgcggca ccggtttgct caagcccgac
     5821 gtggtgttct ttggcgagaa cgtgccgcgc gagcgtgtcg aagcggccca tgcggcgctg
     5881 gcgcgagccg acgccgtgct cgtcgccggg tcgtcgctga tggtgtattc gggctttcgc
     5941 ttcgtgcagg cggcggtcga tcgcggcctg ccggtggtgg ccatcaacca gggccgcaca
     6001 cgcgccgatg gcctgctggc gctgaagctg cagcaggatg tcggccagac cctggtggac
     6061 ttggcgcatc aactgggggt ggcggtgagc cgttggcagt cgccggtgat ttcgggctct
     6121 tgatactttc ttgatgccca ccccacgaat ctttgccacg ccttgaagcg cccatttcta
     6181 cgatgccttc attgacttga aggaggtgga aatgacgaat gcaaagccct gccaacgggg
     6241 ccgcggggag ggcgcatgac cggcctgacc ctgatccatg ccctggccgg cgccttggcg
     6301 ctggggctct tcgtgtacct gggctacgcc ttgctgcggc ccgagaactt ttgaaccgga
     6361 ggcgccctgt gcaagacctt ctgttcatcg gcctgtcggc cctgttcttc tgcgccacgg
     6421 cggcactggt gctcgccctg cggcggctgt gagggggagc catggaactc at
//
LOCUS       DAOMTQ010000166         6436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2811_length_6436_cov_22.640652, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000166 DAOMTQ010000000
VERSION     DAOMTQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2811_length_6436_cov_22.640652"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PK518_13335"
     CDS             complement(<1..1100)
                     /locus_tag="PK518_13335"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM49938.1"
                     /translation="MHTSSAPTPAALSGVRVIEMGQLIAGPFCGKTLGEFGADVVKIE
                     AVGSGDPLRNWRLIKEGTSVWWQVQSRNKKSVALDLRQAEAQAIARRLIAEADVLIEN
                     FRPGTLEGWGMAPEELHALNPGLVILRISGYGQTGPYRDLPGFGLIGEAMGGLRHLTA
                     EPGRVPVRVGVSIGDTLAALHGVIGVMMALYHRKVNGGAGQVIDVALHEAVFNCMESL
                     IPEYSAFGAVREPAGSALPGIAPSNAYPCRDGWVLVAGNGDSIFKRLMATIDRQDLAD
                     DAQLADNAGRVARVAEIDAAIGTWTGQHSVAQVMERLAAARVPAGKVYTAKDIAEDPH
                     YQARAMLLQQQTRDGYSITVPGVVPKLSATPGT"
     gene            1226..2128
                     /locus_tag="PK518_13340"
     CDS             1226..2128
                     /locus_tag="PK518_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM49939.1"
                     /translation="MKLDPLSLRLFVAVMEDGAIARAAAREHIAPSAASRRLAELEGQ
                     LRVELFARSNRGAEPTAAAYALLNLARGVLNDLDGIATQMRDYRAGVRGHVRVVANIS
                     AITQFLPDELQRFMAAHPQVDVRLQEQISSAVAQSVAENAADVGILNHGGYGERVTLL
                     PYREDELVLIVPVGHALARRQRVRLAEALPHPFVGMHPGSAINNLLTRCAAELDMPLK
                     LRMQVTSYDALCLMVSAGLGVAVLPRGSAQNLRGSLALRAITLAEPWARRRLSLCVRA
                     QESLSGAAGLLVAHLRAQIAEATP"
     gene            2125..3207
                     /gene="earP"
                     /locus_tag="PK518_13345"
     CDS             2125..3207
                     /gene="earP"
                     /locus_tag="PK518_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HRM49940.1"
                     /translation="MTGPLRWDIFCQVIDNHGDIGVCWRLAAELGARGHCVRLWVDDA
                     SALAWMAPQGAAGVQVLPWPSTAPPDGPGDVVVEAFGCEIAPKFIAAIAHHTCANGQK
                     PLWINLEYLSAEGYVERCHRLPSPLMNGPGKGLTRWFFYPGFTPHTGGLLRERDLAAR
                     QAAFDRQAWRARHGIAPDDLAVSLFCYEPPALPQLLAQLHARGDARLLVTPGRAQAAV
                     LHQIGLQPAWNKREQLFISYLEACPQPAFDEMLWASDLNLVRGEDSLVRALWAGQPLV
                     WQIYPQHDDAHHAKLAAFLDWLQAPASLRRWHALWNGLEAAPALPSLDSATLAEWRAC
                     VQAARARLLAQPDLLTLLIGFVNEKS"
     gene            3362..3916
                     /gene="efp"
                     /locus_tag="PK518_13350"
     CDS             3362..3916
                     /gene="efp"
                     /locus_tag="PK518_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRM49941.1"
                     /translation="MKIAQEIRAGNVIMQGKDPMIVLKTEYARGGRGAATVRMKLKSL
                     LGNMGTEVVFKADDKIDNVILDKKDCTYSYFADPMYVWMDADYNQYEVEAGNMGDAIS
                     YLEDGMTAEVVFYDGKAISVELPTSVEREITWTEPAVKGDTSGKVLKPAKIGTGFEVA
                     VPLFVGQGDKIEIDTRTGEYRKRV"
     gene            complement(4024..4506)
                     /locus_tag="PK518_13355"
     CDS             complement(4024..4506)
                     /locus_tag="PK518_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49942.1"
                     /translation="MILLKTAAGQQAFKERHGALSLRQRSAFLLFDGQRSLAQVLEAT
                     AALGISWDDVRNMVDAGLLELAEAGPAARNPGSAKPPVPPVAMSGGVGSERSTSERYQ
                     AGYLIATELTAALGLRGFRLHLAVEGATGFESLAALAPRIREAVGADKCARLEHALFG
                     "
     gene            complement(4623..>6436)
                     /locus_tag="PK518_13360"
     CDS             complement(4623..>6436)
                     /locus_tag="PK518_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HRM49943.1"
                     /translation="WMSAASFISLSGALYLQGFSGTPDQPGGLAYLLGWTGGFVLVAM
                     LVAPHLRAMKLYTIPDFFQVRFGGCWPRIIAALSAVLCSFTYVVAQIYGVGLIASRLT
                     GVQFEIGIMLGLGGVLLCSFLGGMRAITWTQVAQYMVILLAFLIPVSWLAYKQLGNPV
                     APIVAGKQLGKIAQLEDGLLASEVERQVVAAYLQRARDYEARLTDVPAALAREREATR
                     ARLQRLRAQNADVGLIVAASRELASMPRDAASARERWTREMLANYERARELGGLPRHS
                     QAYAGDPDGTPAERQAFDHSRRNFLALMFCLMVGTAGLPHLLTRYYTVPSVGAARTSV
                     AWSLFFVALLYLSAPALAVLVKYEVMNNLVGSSFDQLPNWMAQWARVDASLLSVEDMN
                     GDGIVQFGEIRFGADLIMLATPEIAGLPYVISGLVAAGGLAAALSTADGLLLTISNAL
                     VRDLYFQQSGRKASPEQRVILTKFTLLSVALSAAFVAALKPSEILPMVSASFSIAASA
                     FVPAMVLGIFWRGTTRRGAVAGMLAGLSVTMYYLLSQVDGVREVVPDMLLADGLWFGI
                     SPISSGVFGVPTGLLVTWLVSLVTRPGRVAVVIPDGL"
BASE COUNT         1091 a         2283 c         2100 g          962 t
ORIGIN      
        1 gtgcccggcg tggccgagag cttggggacg acgcccggca cggtgatgct gtagccatcg
       61 cgcgtctgct gttgcagcag catggcgcgc gcctggtaat gcggatcctc ggcgatgtcc
      121 ttggccgtgt agaccttgcc ggcgggcacg cgcgccgcgg ccagccgctc catcacctgg
      181 gccacgctgt gctggcccgt ccaggtgcca atggcggcat cgatctcggc cacgcgcgcc
      241 acgcggccgg cgttgtcggc cagttgcgcg tcgtcggcca ggtcctgccg gtcaatcgtg
      301 gccatcaggc gcttgaagat gctgtcgcca ttgccggcca ccagcaccca gccgtcccgg
      361 caggggtagg cgttggacgg cgcaatgccc ggcagcgcgc tgcccgcggg ctcgcgcacg
      421 gcgccaaagg cgctgtactc ggggatcagg ctttccatgc agttgaacac cgcctcgtgc
      481 agcgccacgt cgatcacctg gcccgcgcca ccattcacct tgcggtggta cagcgccatc
      541 atcacgccga tcacgccatg cagcgccgcc agcgtgtcgc caatcgacac accaacccgc
      601 accggcacgc ggccgggctc ggccgtcaag tggcgcaggc cgcccatggc ctcgccgatc
      661 aggccaaagc ccggcaggtc gcggtaggga ccggtctggc cgtagcccga gatacgcagg
      721 atgaccaggc ccgggttgag cgcgtgcaat tcctcgggcg ccatgcccca gccctccagc
      781 gtgccggggc ggaagttctc gatcagcaca tcggcctcgg caatcaggcg gcgcgctatg
      841 gcctgggcct cggcctggcg caggtccagc gccaccgact tcttgttgcg cgactgcacc
      901 tgccaccaga ccgaggtgcc ctccttgatc aggcgccagt tgcgcagcgg gtcgccgctg
      961 cccacggcct ctatcttcac gacgtcggcg ccaaactcac ccagcgtctt gccgcagaac
     1021 ggcccggcga tgagctggcc catctcgatc acgcgcacgc cggacagggc cgcgggcgtg
     1081 ggggcgctgc ttgtatgcat gttgtgtctc ctgaaaatcc ggcgcgcatc atgcgccagc
     1141 gcctgcccag ggcaaaacgc tatggcacga ggctgccatc gcaaaccggc atggcgggcg
     1201 ccggagcgca gtaccatcgc cggccatgaa gctcgatccg ctctccctgc gcctgttcgt
     1261 cgccgtgatg gaggacggcg ccatcgcccg tgccgccgcg cgcgagcata ttgcgccgtc
     1321 ggccgccagc cgccgcctgg ccgagctgga gggccagctg cgcgtggagc tgttcgcgcg
     1381 cagcaaccgc ggcgccgagc ccacggccgc ggcctacgcg cttttgaacc tggcgcgcgg
     1441 cgtgcttaac gacctggacg gcatcgccac gcagatgcgc gactaccgcg ccggcgtgcg
     1501 cggccatgtg cgcgtggtgg ccaacatctc ggccatcacc cagttcctgc ccgatgagtt
     1561 gcagcgcttc atggccgcgc acccgcaggt cgatgtgcgc ctgcaggagc agatcagcag
     1621 cgccgtggcg cagtcggtgg ccgagaacgc ggccgacgtg ggcatcctca accacggcgg
     1681 ctatggcgag cgcgtcaccc tgctgcccta ccgcgaggac gagctggtgc tcatcgtgcc
     1741 cgtgggccat gcgctggcgc gcaggcagcg cgtgcgcctg gccgaggccc tgccccaccc
     1801 cttcgtcggc atgcacccgg gcagcgccat caacaacctg ctgacgcgct gcgcggccga
     1861 gctggacatg ccgctgaagc tgcgcatgca ggtcaccagc tacgacgcgc tgtgcctgat
     1921 ggtctcggcc ggcctgggcg tggctgtgct gccgcgcggc agcgcgcaaa acctgcgcgg
     1981 ctcgctggcg ctgcgcgcca tcaccctggc cgagccctgg gcgcgccggc gcctgtcgct
     2041 gtgcgtgcgc gcgcaggagt cgctctcggg cgcggcgggc ctgctggtgg cgcacctgcg
     2101 cgcccagatc gcggaggcca cgccatgact gggccgctgc gctgggacat cttctgccag
     2161 gtcatagaca accatggcga catcggcgtg tgctggcgcc tggcggccga gctgggcgcg
     2221 cgcggccact gcgtgcgcct gtgggtggac gacgccagcg ccctggcctg gatggcgccg
     2281 cagggcgcgg ccggcgtgca ggtgctgccc tggcccagca ccgcgccgcc ggacggcccg
     2341 ggcgatgtgg tggtggaggc cttcggttgc gagattgctc cgaaattcat agctgctatc
     2401 gctcaccata cctgcgcaaa cggccaaaaa cccttgtgga tcaacctgga atacctgtcg
     2461 gccgagggct atgtggagcg ctgccatcgc ctgccatcgc ccctgatgaa cggcccgggc
     2521 aagggcctga cgcgctggtt cttctacccc ggcttcacgc cgcacaccgg cggcctgctg
     2581 cgcgagcgcg acctggcggc gcgccaggcg gcctttgacc gccaggcctg gcgcgcgcgc
     2641 cacggcattg cgccggatga tctggccgtg tcattgtttt gttacgagcc gccggccctg
     2701 ccccagctgc tcgcgcaact gcatgcacgc ggcgacgctc ggctcttggt cacaccgggc
     2761 cgggcccagg ctgctgtttt gcatcaaatt ggcctgcaac ccgcatggaa caagcgcgaa
     2821 cagctattta tttcatatct tgaagcctgc ccgcaaccgg cctttgacga aatgctctgg
     2881 gcctcggatc tgaacctggt gcgcggcgag gactcgctgg tgcgcgccct ctgggccggc
     2941 cagcccttgg tctggcagat ctacccgcag catgacgacg cgcaccacgc caagctggcg
     3001 gcctttctgg actggctgca ggcgcccgct tcgctgcggc gctggcatgc gctgtggaat
     3061 gggttggagg ccgcgcctgc gctgccgtcg ctcgatagcg ccaccctggc cgaatggcga
     3121 gcctgcgtgc aggcggcacg cgcgcggctg ctggcacaac ccgatttatt gaccttactc
     3181 atagggtttg tgaacgagaa aagctagaat gcttggcttt gcgccatgaa aaggcggcac
     3241 tccccgtgcc ccaccggcgc gccccgccgc ggaacatgcg ccggggtgga aggcagccct
     3301 cccggccacg cccacccgcc ccgccctgtg gagcggccca acccttcgct gaagccaaac
     3361 catgaaaatc gctcaagaaa tccgcgccgg caacgtgatc atgcagggca aagacccgat
     3421 gatcgtcctg aagaccgagt acgcccgcgg cggccgcggc gccgccaccg tacgcatgaa
     3481 gctcaagtcc ctgctgggca acatgggcac cgaagtcgtg ttcaaggccg acgacaagat
     3541 cgacaacgtc atcctggaca agaaggactg cacctactcc tacttcgccg acccgatgta
     3601 tgtgtggatg gacgccgact acaaccagta cgaggtggaa gccggcaaca tgggcgacgc
     3661 catcagctac ctggaagacg gcatgaccgc cgaagtcgtg ttctatgacg gtaaggccat
     3721 ctcggtcgag ctgcccacca gcgtggagcg cgaaatcacc tggaccgagc ccgccgtcaa
     3781 gggcgacacc tccggcaagg ttctgaagcc cgccaagatc ggcaccggct tcgaagtcgc
     3841 cgtgccgctg tttgtgggcc agggcgacaa gatcgaaatc gacacccgca ccggcgaata
     3901 ccgcaagcgc gtctaagcca cctgctcacc acaaaaaagg aacgtcacga cgttcctttt
     3961 tttatgcgtg acgccggcgc agtgcgggca atagatgccg cctgcgcatc ggtcgtaaga
     4021 ctgtcagcca aacaatgcat gctccaggcg cgcgcatttg tccgcgccca cggcctcgcg
     4081 gatcctgggc gccagggccg ccagcgactc gaagccggtc gcaccctcca cggccagatg
     4141 caggcgaaag ccgcgcagcc ccagggctgc ggtgagttcg gtggcgatca ggtagccggc
     4201 ctggtaacgc tcgctcgtcg agcgctcgct gcctacgccg cccgacatcg ccacgggcgg
     4261 tactggcggc ttggcgctgc cggggttcct ggcggctggc cccgcttcgg ccagctccag
     4321 cagcccggca tcgaccatgt tgcgcacatc gtcccaggat atgcccaggg ctgcggtggc
     4381 ctccagcacc tgcgccaagc ttcgctggcc atcaaacaag agaaaggccg aacgctggcg
     4441 cagcgaaaga gcgccatggc gctccttgaa cgcctgctgt ccggccgcag tcttgagcaa
     4501 aatcatgcct gtcccttgct gtgcattgca tccaggcgca gtagcgccgc aaaggtttca
     4561 gtttgccacg tttgttttca attgtgaacc tggcggcgtc gcgccgattg caccggccca
     4621 agtcatagcc cgtccggaat caccacggcc accctgcctg gccgcgtaac caggctcacc
     4681 agccaggtca cgagcaggcc tgtgggcacg ccaaacacgc cagaggagat ggggctgatg
     4741 ccgaaccaca gcccatcggc aagcagcatg tcgggaacga cctcgcgcac cccgtccacc
     4801 tgggacagca ggtagtacat ggtgaccgac aggcccgcca gcatgcccgc caccgcgcct
     4861 cggcgcgtgg tgccgcgcca gaagatgccc agcaccatgg ccggcacaaa ggccgatgcg
     4921 gcaatcgaga acgaggccga gaccatgggc aggatctcgg agggtttcag cgccgccacg
     4981 aaggccgccg acagggccac cgagagcaac gtgaacttgg tcagaatcac gcgctgctcg
     5041 ggcgatgcct tgcgcccgct ttgctgaaag tacaggtcac gcaccaaggc attgctgatg
     5101 gtcagcagca ggccatcggc ggtggacagc gccgccgcca ggccacccgc ggccaccagg
     5161 cccgaaatga cgtaaggcag gccggcgatc tcgggcgttg ccagcatgat caggtcggcg
     5221 ccaaagcgga tctcgccaaa ctgcacgatg ccgtcgccat tcatgtcctc caccgacaac
     5281 agcgaggcat ccacccgcgc ccattgcgcc atccagttgg gcagctggtc gaagctgctg
     5341 cccaccaggt tgttcatgac ctcgtacttc accagcaccg ccagggcggg ggcgctcagg
     5401 tacagcagtg ccacgaagaa cagcgaccag gccaccgagg tgcgcgccgc ccccaccgac
     5461 ggcacggtgt agtagcgcgt gagcaaatgt ggcaggccgg ccgtgcccac catcaggcag
     5521 aacatcagcg ccaggaagtt gcgccgcgaa tgatcgaagg cttggcgctc ggccggcgtg
     5581 ccgtcggggt cgccggcata ggcctggctg tggcgcggca gaccgccgag ctcgcgcgcg
     5641 cgctcatagt tggccagcat ctcgcgcgtc cagcgctcgc gcgcgctggc cgcgtcacgc
     5701 ggcatggagg ccagctcgcg gctggctgcc acgatcaggc ccacgtcggc gttctgcgcg
     5761 cgcaggcgct gcaggcgtgc acgcgtggcc tcgcgctcac gcgccagcgc cgcgggcacg
     5821 tcggtcaggc gcgcctcgta gtcgcgcgcg cgctgcaggt aggcggccac cacctggcgc
     5881 tccacctcgg aagccagcag gccgtcttcc agctgcgcga tcttgcccag ttgcttgccg
     5941 gcaacgatgg gtgccaccgg gttgcccagc tgcttgtagg ccagccagga cacgggtatc
     6001 aagaacgcca gcaggatcac catgtactgc gccacctgcg tccaggtaat cgcccgcatg
     6061 ccgcccagaa aggaacacag cagcacgccg cccaggccca gcatgatgcc gatctcgaac
     6121 tgcacgcccg tcaggcgcga tgcaatcagg cccacgccgt agatctgcgc caccacatag
     6181 gtgaacgagc acagcacggc cgacaacgcc gcgatgatgc gcggccagca cccgccaaag
     6241 cgcacctgga agaaatccgg gatggtgtag agcttcatgg cccgcaggtg cggcgccacc
     6301 agcatcgcca cgagcacgaa gccgcccgtc cagcccagca gataggccag gccgccgggt
     6361 tgatccggtg tgcccgaaaa accctgcagg tacagcgccc ccgacaggct gatgaacgag
     6421 gccgcgctca tccagt
//
LOCUS       DAOMTQ010000167         6416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2826_length_6416_cov_29.021852, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000167 DAOMTQ010000000
VERSION     DAOMTQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2826_length_6416_cov_29.021852"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK518_13365"
     CDS             complement(<1..344)
                     /locus_tag="PK518_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRM49944.1"
                     /translation="MSNPVAQACSFLFVPATHPERFQKALASGADMAIADWEDAVAPA
                     DKAGARAALLQAAAAMDAAQRARLLVRINAEGTPWFADDLQALATLAAQGYAGAVVPK
                     AESAATLQAVAQA"
     gene            complement(356..1342)
                     /locus_tag="PK518_13370"
     CDS             complement(356..1342)
                     /locus_tag="PK518_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HRM49945.1"
                     /translation="MKTFTFSPSRIAALLALGLATTAQAAWPDKPVTLVVPYSAGGPT
                     DVVARVLAVPMGQSLGQTVVVENTVGAGGTIAPARVAKARNDGYTILIHHMGMATAPA
                     LYKKLPYNPLTDFEYIGQVLDVPMTLLSRKDFPANNFSELLEYVKKNQDKVSLANAGI
                     GAVSQLCGMLFMDRIGVKLTTVPYKGAGPAMNDLMGGQVDLLCDQTTQTAPVIADGKR
                     AKVFGVTTAQRLASMPNIPTLDEQGLKGFDLKVWHGMYAPKGTPKEVIDQLNKALNMA
                     LKDDNVRKRIAELSSDLVSPDKATPESLRKQLESETARWAKVIKAAGVSAEQ"
     gene            complement(1392..2765)
                     /locus_tag="PK518_13375"
     CDS             complement(1392..2765)
                     /locus_tag="PK518_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRM49946.1"
                     /translation="MKQETQATIHPLAQFAATLQWSDVPEPVQRRAEDLWVDWFGSVL
                     AGCKARPVASITRFALSQGPAQGPSEVIAHGATSSPMMAALANAAASHFAEQDDVHNG
                     SVFHPAAVVFPPALAVAQSIGASGAELMAACVAGYEVGIRVGEFLGRSHYKIFHTTAT
                     AGTLAAAAAVGRLLKLTPAQMQHAFGSAGTQSAGLWEFLRTAADSKQLHTAHAASAGL
                     MAAYLAQDGFTGAQDIFTGPQGLAAGMSTDANPAKLTDGLGQRWATAETSFKWHASCR
                     HTHPAADALLQVMRQHGLQTADIAQVTCHVHQGAIDVLGPVVSPATVHQSKFSMGTVL
                     ALAARFGHAGLSEFDADYLAPATVALRDKVSMVLDAEVDAAYPQRWIGKVTVATTDGR
                     ALHGRVDEPKGDPGNTLSRGEITAKALRLAAYGGAVSAAQAEAAVASLWQVAQWPRVG
                     QLLPTDA"
     gene            complement(2950..4131)
                     /locus_tag="PK518_13380"
     CDS             complement(2950..4131)
                     /locus_tag="PK518_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRM49947.1"
                     /translation="MTRPLDGITVISLEHAVAAPFATRQLADLGARVIKVERPGSGDF
                     ARGYDQRVHGQSSHFTWLNRNKESLALDVKQPQAKEALLQLLKTADVLVQNLAPGAAA
                     RMGLSYAALKEHNPKLIVCDISGYGADGPYRDKKAYDLLIQSEAGFLSVTGTPDTPAK
                     SGISVADIAAGMYAYSNILSALLLRGKTGEGSQIDVSMLEAMGEWMGYPLYYAFEGAP
                     PPPRTGASHASIYPYGPFQAGDGGTVMLGLQNEREWQAFCDGVLQRPEVAADPRFCSN
                     ALRNQHRAELQALILETFAPLTAAQVVERLDAASIANARVNDMADLWAHPQLKARGRW
                     RSVGTPAGAVPALLPPGVNSAFDYRMEAVPAVGQHNAAILAELGWSAEQIDALQAPQA
                     I"
     assembly_gap    4181..4280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4301..5467)
                     /locus_tag="PK518_13385"
     CDS             complement(4301..5467)
                     /locus_tag="PK518_13385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM49948.1"
                     /translation="MIQHTGSNNHHEIRDAIRALCAEFPDEYFRKVDESRAYPAEFVD
                     ALTKAGWMAALIPHEYGGSGLGLTEASVIMEEINRCGGNSGACHGQMYNMGTLLRHGS
                     TAQKEKYLPKIASGEWRLQSMGVTEPSTGTDTTKIKTTAVKKDGRYVINGQKVWISRI
                     QHSDWMILLARTTPLAEVTKKSEGMSIFMVDLREAEKSGMTVRPILNMVNHETNELFF
                     ENLEIPEENLIGQEGKGFKYILDGLNAERTLIAAECIGDGYWFLDRVTNYVRDRQVFG
                     RPIGQNQGVQFPIADAFIEVEAANLMRWKACELFDKHEPMGAQANMAKYLAAKASWEA
                     ANACLQFHGGFGFACEYDVERKFRETRLYQVAPISTNLIYSYVAEHILGLPRSF"
     gene            complement(5540..6409)
                     /locus_tag="PK518_13390"
     CDS             complement(5540..6409)
                     /locus_tag="PK518_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRM49949.1"
                     /translation="MDPAALAHLQTWHGKSETLSDSFTAMPVAALSATLDRDDPEPAL
                     GTVVPPLWHWLYFLPHARASEIGPDGHPKRGGFLPPVPLPRRMWAGGRLSWEPANPLC
                     VGQQVQRRSTIQSVSHKAGRSGELLFVLVEHKYLNAHGLALTEEHDIVYRSAAQPGEA
                     APAPQKPPLAGQAAWSRTIVPDDVLLFRYSALTFNGHRIHYDRKYVTEVEGYPGLIVH
                     GPLIATLLLDLLRRQLPDAQVARFEFRAVRPTFDLQPFSVHGKPSADGKTVELWAQDH
                     EGWLTMQGQATLA"
BASE COUNT          907 a         2064 c         2222 g         1123 t
ORIGIN      
        1 gcttgggcca cggcctgcag cgtggccgcg ctctcggcct tgggcaccac ggcgccggcg
       61 tagccctggg ccgccaacgt ggccagcgcc tgcaggtcgt cggcaaacca gggcgtgccc
      121 tcggcgttga tgcgcaccag caggcgcgcg cgctgtgcgg catccattgc ggccgcggcc
      181 tgcagcaggg cggcgcgggc gccggccttg tcggccggtg ccacggcgtc ctcccagtcg
      241 gcgatcgcca tgtcggcgcc gctggccagg gccttctgaa aacgctcggg atgcgtggcg
      301 ggcacgaaca aaaaggagca ggcctgggcc accgggttcg acatggcggc ctgcctcatt
      361 gctcggccga cacgccggcc gccttgatga ccttggccca gcgcgccgtc tcgctctcca
      421 gctgcttgcg caggctctcg ggcgtggcct tgtcgggcga gaccaggtcg gagctgagct
      481 cggcgatgcg cttcctgacg ttgtcgtcct tcagcgccat gttcagcgcc ttgttcagct
      541 ggtcgatgac ctccttgggc gtgcccttgg gtgcgtacat gccgtgccac accttgaggt
      601 cgaagccctt caggccctgc tcgtccagcg tggggatatt gggcatggac gccaggcgct
      661 gggccgtggt cacgccgaac accttggcgc gcttgccgtc cgcaatcacg ggcgcggtct
      721 gcgtggtctg gtcgcacagc agatcgacct ggccgcccat caggtcgttc atggccgggc
      781 cggcgccctt gtagggcacg gtggtcagct tgacgccgat acggtccatg aacagcatgc
      841 cgcacagctg cgacacggca cctatgccgg cgttggccag gctcaccttg tcctggttct
      901 tcttcacata ctccagcagc tcggagaagt tgttggccgg aaagtccttg cgcgacagca
      961 gggtcatcgg cacatccagc acctggccta tgtactcaaa gtcggtcagc gggttgtagg
     1021 gcagcttctt gtacagcgcc ggggccgtgg ccatgcccat gtggtgaatg aggatggtgt
     1081 agccgtcgtt cctggccttg gccacgcgcg ccggcgcgat ggtgccgccc gcgcccacgg
     1141 tgttctccac caccaccgtc tggcccagcg actggcccat gggcacggcc agcacgcgcg
     1201 ccaccacgtc ggtggggccg ccggcgctgt agggcacgac cagggtgacg ggcttgtcgg
     1261 gccaggcggc ctgggccgtg gtggccaggc ccagggccag cagggcggcg atgcggctgg
     1321 gcgagaaggt aaacgtcttc atggctttgt ctccgatgtt gtggatgtga gaaatggcac
     1381 cactgtaagc gtcatgcgtc cgtgggcagt agctggccca cgcgcggcca ttgcgccacc
     1441 tgccacagag aggcgacggc ggcctcggcc tgggccgcgc tcaccgcgcc gccataggcc
     1501 gccaggcgca gcgccttggc ggtgatctcg ccgcgcgaga gcgtgttgcc ggggtcgccc
     1561 ttgggttcgt ccacgcggcc gtgcagtgcg cgcccatcgg tggtggcgac cgtgaccttg
     1621 cctatccagc gctgcgggta ggcggcgtcc acctccgcgt ccagcaccat gctcaccttg
     1681 tcgcgcaggg ccacggtagc gggggccagg tagtcggcat cgaattcgct caggcccgca
     1741 tggccaaagc gcgcggccag cgccagcacc gtgcccatcg agaatttgct ctggtgcacc
     1801 gtggccggcg agaccaccgg gcccagcacg tcgatggcgc cctggtgcac atggcaggtg
     1861 acctgggcga tatcagcggt ttgcaggcca tgctggcgca tcacctgcaa cagcgcgtcg
     1921 gccgcggggt gcgtgtggcg gcaggaggca tgccacttga agctggtctc cgccgtggcc
     1981 cagcgctggc ccaggccgtc ggtgagcttg gcgggattgg catccgtgga catgccggcg
     2041 gccaggccct gcggcccggt gaagatgtcc tgcgcgccgg taaagccgtc ctgggccagg
     2101 taggccgcca tcagccccgc gctggcggcg tgcgccgtgt gcagctgctt gctgtcggcg
     2161 gcggtgcgca agaactccca caggcctgcc gactgcgtgc cggccgagcc aaaggcgtgc
     2221 tgcatctgcg ccggcgtcag tttgagcagc cgccccacgg cggccgccgc cgccagcgtg
     2281 ccggccgtgg ccgtggtgtg gaagattttg taatgactgc ggcccaggaa ctcgcccacg
     2341 cgtatgccga cctcgtagcc cgccacgcag gcggccatca gctcagcgcc gctggcgcca
     2401 atgctttgcg ccacggccaa cgctggcggg aagaccaccg ccgccgggtg gaacaccgag
     2461 ccgttgtgca cgtcgtcctg ctcggcaaag tggctggcgg cggcattggc cagggcggcc
     2521 atcatgggcg agctggtggc gccatgggcg atgacctcgc tggggccttg tgccgggcct
     2581 tgcgacagcg caaagcgcgt gatgctggcg acgggacgcg ccttgcagcc ggcgagcacc
     2641 gagccgaacc aatcgaccca caggtcttcg gcgcggcgct gtacgggctc gggtacgtcg
     2701 ctccactgca aggtggcggc gaactgggcc agggggtgga tggtggcttg ggtttcttgc
     2761 ttcattttgg actccaggac aggcgggtca agcgccggta gctatctttc tgaaaatggt
     2821 tgccttgtgc gagtggcccc tcacccctcc ctctccccag aggggcgagg gcgatagctt
     2881 ggctgtggct ttcactccct ctccctctgg gagagggtag gggtgagggc cggctacggt
     2941 gttcatcggt caaatcgcct gcggagcctg cagcgcgtcg atctgctccg ccgaccatcc
     3001 cagctcggcc aggatggcgg cgttgtgctg gcccacggcg ggcacggcct ccatgcggta
     3061 gtcgaaggcg ctgttcacgc ccggcggcaa cagggccggc accgcgccgg cgggcgtgcc
     3121 cacgctgcgc cagcgcccgc gcgccttgag ctgcgggtgc gcccacagat ccgccatgtc
     3181 gttcacgcgg gcgttggcta ttgacgcggc gtccaggcgc tccaccacct gggcggcggt
     3241 gagcggcgca aaggtttcca ggatcagtgc ctgcagctcg gcgcggtgct ggttgcgcag
     3301 ggcgttggag cagaagcgtg ggtcggctgc gacctcgggg cgttggagga cgccgtcgca
     3361 gaacgcctgc cattcgcgct cgttctgcag gcccagcatc accgtgccac cgtcgcctgc
     3421 ctggaacggc ccgtaggggt agatgctggc gtgcgatgcg ccggtgcgcg gcggcggtgg
     3481 cgcgccttca aacgcgtagt acagcgggta gcccatccac tcgcccatgg cttccagcat
     3541 ggagacatcg atctggctgc cctcccccgt cttgccgcgc agcagcaggg ccgagaggat
     3601 gttgctgtag gcgtacatgc cggcggcgat gtcggccacc gagatgccgg acttggctgg
     3661 cgtgtccggc gtgccggtga cggacaggaa gccggcctcg ctctggatca acaggtcgta
     3721 ggccttcttg tcgcggtacg ggccgtcggc gccatagccg ctgatgtcgc agacgatcag
     3781 cttcgggttg tgttctttca gggccgcgta ggacagcccc atgcgcgccg cggcgccggg
     3841 cgccaggttc tgcaccagca cgtcggccgt cttcaatagc tgcaacagcg cctcttttgc
     3901 ctggggttgt ttcacgtcca gcgccaggct ctccttgttg cggttgagcc aggtgaagtg
     3961 cgacgactgg ccgtgcacac gctggtcgta gccgcgcgcg aagtcgccgc tgccggggcg
     4021 ctcgaccttg atcacgcgcg cgcccaggtc ggcgagctgg cgcgtggcaa agggcgcggc
     4081 gacggcgtgt tcgagggaga tgacggtgat gccgtcgagg gggcgggtca tgaagggctc
     4141 ccgtgcaata tcaaaacagg gcgggctgtt tctccctcgc nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn gggagaggga gtgaaagccg tcagaacgat cgcggcaggc
     4321 ccaggatgtg ctcggccacg taggaataga tcaggttggt ggagatcggc gccacctggt
     4381 acagccgcgt ctcgcggaac ttgcgctcca cgtcgtattc gcaggcaaag ccgaagccac
     4441 cgtggaactg caggcaggcg ttggccgcct cccagctggc cttggccgcc aggtacttcg
     4501 ccatgttggc ctgtgcgccc atgggttcat gcttgtcgaa cagctcgcag gccttccagc
     4561 gcatcagatt cgcggcctcg acctcgatga aggcgtcggc gatcgggaac tgcacgccct
     4621 ggttctggcc gatcgggcgg ccaaacacct ggcggtcgcg cacatagttg gtcacgcgat
     4681 ccaggaacca gtagccgtcg ccaatgcact cggcggcgat cagcgtgcgc tcggcgttca
     4741 ggccgtccag gatgtacttg aagcccttgc cctcctggcc gatcaggttc tcctcgggga
     4801 tctccaggtt ttcgaagaac agctcgttgg tctcgtggtt gaccatgttg agaatcgggc
     4861 gcaccgtcat gccgctcttc tcggcttcgc gcagatccac catgaagatc gacatgccct
     4921 cgctcttctt cgtcacctcg gccagcggcg tggtgcgcgc cagcagaatc atccagtcgc
     4981 tgtgctggat gcggctgatc cagacctttt gcccgttgat gacgtagcgg ccgtcttttt
     5041 tcaccgccgt ggtcttgatc ttggtggtgt cggtgccggt gctgggctcg gtcacgccca
     5101 tcgattgcag gcgccattcg ccggatgcaa tcttcggcag gtacttttcc ttctgcgccg
     5161 tcgaaccatg gcgcagcagc gtgcccatgt tgtacatctg gccgtggcag gcgcccgagt
     5221 tgccgccaca gcggttgatc tcctccatga tcacgctggc ctcggtcagc cccaggccgg
     5281 agccgccgta ttcgtgcggg atcagcgccg ccatccagcc ggccttggtg agcgcatcga
     5341 cgaactcggc cgggtaggcc ctgctctcgt ccaccttgcg gaagtactcg tcggggaact
     5401 cggcgcacag cgcgcggatc gcgtcgcgga tctcgtggtg gttgttggag ccggtgtgtt
     5461 ggatcatggt ggtctttcta tggttttggc ctctagcgcc cgtccatcaa gcgcaaatag
     5521 ctattgattc aatagtgttt caggccagcg tggcctggcc ctgcatggtc agccagccct
     5581 cgtgatcctg ggcccacagc tccacggtct tgccgtcggc cgagggcttg ccgtgtacgc
     5641 tgaagggctg caggtcgaag gtcggccgca cggcgcgaaa ttcaaagcgc gccacctggg
     5701 catcgggcag ctggcggcgc agcaggtcca gcagcagcgt ggcaatcagc gggccgtgca
     5761 cgatcaggcc ggggtagccc tcgacctcgg tgacgtactt gcggtcgtag tggatacggt
     5821 ggccgttgaa ggtcagggcc gagtagcgaa acagcagcac gtcatcgggc acgatggtgc
     5881 gcgaccaggc agcctggccg gccaacggcg gcttttgcgg cgcgggcgcc gcctcacccg
     5941 gctgggccgc actgcgatag acgatgtcgt gctcctctgt cagcgccagg ccatgggcgt
     6001 tgagatactt atgctccacc agcacgaaca gcagctcgcc ggaacgcccg gccttgtgcg
     6061 agacggactg tatggtcgag cggcgctgca cctgctggcc cacgcacagc gggttggccg
     6121 gctcccagga caggcgtcca ccggcccaca tgcgccgcgg cagcggcacg ggcggcagaa
     6181 aaccgccgcg cttcggatgg ccatcgggcc cgatctcgct ggcgcgcgca tggggcagga
     6241 agtacagcca atgccacagc ggcggcacga ccgtgcccag ggccggttcg gggtcgtccc
     6301 ggtccagcgt ggccgacagg gccgccacgg gcatggcggt gaagctgtcg ctcagagttt
     6361 cgctcttgcc atgccaggtc tgcaggtggg cgagggcggc ggggtctatg gtggtg
//
LOCUS       DAOMTQ010000168         6368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2857_length_6368_cov_32.513702, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000168 DAOMTQ010000000
VERSION     DAOMTQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2857_length_6368_cov_32.513702"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PK518_13395"
     CDS             <1..648
                     /locus_tag="PK518_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49950.1"
                     /translation="ATPSHAASPMVTDDARIVDARACQVESWVTSYRGSTEYSIQPAC
                     NITGNLELALGSTRTNAHGHSQTTDVVLQGKMVLRPLQANGWGLALAAGSVGHPQAAA
                     RRDWYAYAPVSFSLRDDALQVHANVGWLRDGAARRDRLTWGLGTEIQLSARAWLLAET
                     FGQNQGRPMHQLGLRYSLVPDRVQLDATYGNRNGTHLRERWFSIGLRLLTPAFLP"
     gene            complement(670..2082)
                     /locus_tag="PK518_13400"
     CDS             complement(670..2082)
                     /locus_tag="PK518_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRM49951.1"
                     /translation="MNAPAHTTQLLARPVPQALLDALSQRFGTQYSSALAVRAQHGRD
                     EGSIEAPPPAAVVFAESRQDVQDAVRLAAQHAVPVIAYAAGSSLEGHLLAVQGGISID
                     VNRMNRVLSIDADDLTVTVQPGITRKQLNEAIKDRGLFFPIDPGADASMGGMAATRAS
                     GTNAVRYGTMRENVLALEVVTASGEAIRTGTRAKKSSAGYDLTRLMVGSEGTLGIFTE
                     VTLRLFPLPEAVSAAICSFPSIEAAVRTVIATIQLGVPIARVELIDANSVRMVNAHSK
                     LDLREEPMLLMEFHGSPSGVREQAETVQALASEHGGQAFEWASTPEERTRLWTARHNA
                     YFAAVQSRPGCRAISTDTCVPISRLADCLLDSVAEADASGIPYFLVGHVGDGNFHFGY
                     LIDPNDVGERERAERLNHQLVTRALAMGGTCTGEHGVGIHKMGFLLDEAGAGAVDMMR
                     AIKRALDPQNILNPGKIFTL"
     gene            complement(2212..2439)
                     /locus_tag="PK518_13405"
     CDS             complement(2212..2439)
                     /locus_tag="PK518_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49952.1"
                     /translation="MPTTQQRRPISPFDARSVMLFAALTMGGALAQAQTSSTPAAQSK
                     HQASRSPAPTGLKLIIGSSARGAEASLKRPA"
     gene            2595..3164
                     /locus_tag="PK518_13410"
     CDS             2595..3164
                     /locus_tag="PK518_13410"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRM49953.1"
                     /translation="MGNRLTQIATRTGDDGTTGLGDNTRVSKASGRPHAMGDVDELNS
                     HIGLLLCESLPADMRELLIDVQHQLFNLGGELSMPGYELLKDDGLLQLDNALARYNAE
                     LPRLQEFILPAGTRAACQAHVCRTVARRAERAVVGLQEGEAMRAAPRQYLNRLSDLLF
                     VLSRVLNRLDAGSEVYWKSERLARQNATE"
     gene            complement(3421..3843)
                     /locus_tag="PK518_13415"
     CDS             complement(3421..3843)
                     /locus_tag="PK518_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRM49954.1"
                     /translation="MPIWKKPLTLELLHSAHANTAIAHLGIEITEIGDDFLRGRVPVD
                     ERTRQPYGLLHGGVSVVLAETLGSMGAFYSCPAGQRAVGLDINANHLRAATSGWVTGT
                     ARPVHIGKTTQVWQIDMVNDEGQLTCVSRITMAVLAPK"
     gene            complement(3907..5442)
                     /locus_tag="PK518_13420"
     CDS             complement(3907..5442)
                     /locus_tag="PK518_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HRM49955.1"
                     /translation="MSSRIQHPGLAQKIMSASDAAALIPAGATVGMSGFTGSGHPKLV
                     PQALAQRIAAEHAAGKPFQVNVWTGASTAPELDGALAKVGGMHQRLPFQSDPLCRNKI
                     NAGELHYLDIHLSHVAQQAWFGFLGRMDVAVVEVLGILPDGRLIPSAAVGNNKTWLDI
                     ADKVILEVNSLPPKEMEGMHDIYYGTAIPPRRQPIHLTQPIERIGEPYLRVDPNKVIA
                     VVETHHGDRDTAFARPDAVSNQIAAHIIDFLAHEVKLGRLPKAMLPLQSGVGNVANAV
                     LAGLLDGPFEDLLGFTEVLQDGMLELIKRGKMAAASATAISLSREALKDFTDNIDFYR
                     QRIVLRPQEISNHPELVRRLGVIAMNAMIEADIYGNVNSTHIMGSGMMNGIGGSGDYA
                     RNGYLSFFVTPSVAKGGDVSCIVPMVSHVDHTEHDTQIIVTEQGLADLRGLSPRQRAQ
                     VIIDKCAHPDWRPMLQDYFDRARRQGAQHTPHLLSEALSWHQRCLETGSMRATEDELA
                     AVA"
     gene            5630..6367
                     /locus_tag="PK518_13425"
     CDS             5630..6367
                     /locus_tag="PK518_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRM49956.1"
                     /translation="MPSTSLCPSCRQPADKRRFAAADGSMLELDLCFACQGLWFDPQE
                     NTRLAPAAVLQLFALLHEHHGDAHHPLSDRLRCPHCRKDLVKGYDLAQGGRYVTYRCA
                     ARHGRFSTFGSFMVEKGFVRHLSTLEIEALARRVGSIACTSCGGTVDIRNDHACPYCR
                     SALALLDPQAVDKALQRHGQAASQAASQAANGQSPEALADALIALERNRTREERERQR
                     ERFEGRREDQFDLVAAGIEFVWSLLRR"
BASE COUNT         1014 a         2111 c         2168 g         1075 t
ORIGIN      
        1 gccaccccga gccatgccgc cagccccatg gtcaccgacg atgcacgcat cgtcgatgcc
       61 agggcctgcc aggtggagag ctgggtcaca agctatcggg ggagtaccga gtactcgatc
      121 cagccggcct gcaatatcac cggcaacctg gagctggccc tgggcagcac gcgcaccaat
      181 gcccatgggc acagccagac caccgatgtg gtgctgcagg gcaagatggt attgcggccc
      241 ctgcaagcca atggctgggg cctggccctg gcggccggca gcgtgggcca tccacaagca
      301 gccgcacgac gcgattggta cgcctatgcg ccggtcagct tctcactgcg cgatgacgcc
      361 ttgcaggtgc atgccaatgt cggctggctg cgcgatgggg cggctcggcg tgatcgtctg
      421 acctggggcc tggggacgga gatccagctg agcgccagag cctggctgct ggccgagacc
      481 tttggccaga accagggccg ccccatgcat cagctggggc tgcgctattc gctggtgccc
      541 gatcgcgtgc agctcgatgc cacctatggc aatcgcaatg gcacgcacct gcgcgagcgt
      601 tggttttcca tcggcctgcg ccttttgacc cccgccttcc tgccgtagcg ggagtagggc
      661 aggcaggcgt cacagcgtaa agatcttccc tggattgagg atgttctgcg ggtcgagcgc
      721 gcgcttgatc gcgcgcatca tgtccaccgc gccggcgccg gcctcgtcca gcaaaaagcc
      781 catcttgtgg atgcccacgc catgctcgcc ggtgcaggtg ccgcccatgg ccagggcgcg
      841 cgtcaccagc tggtggttga gccgctcggc gcgttcgcgt tcgccgacgt cgttggggtc
      901 gatcaggtag ccgaagtgga agttgccgtc gcccacatgg ccgaccagga agtaggggat
      961 gccgctggcg tcggcctcgg ccacggagtc gagcaggcag tcggccaggc ggctgatggg
     1021 cacgcaggtg tcggtactga tggcgcggca gccggggcgg ctttgcaccg cggcaaagta
     1081 ggcgttgtgc cgcgccgtcc acaggcgcgt gcgctcctcg ggcgtgctgg cccactcgaa
     1141 ggcctggccg ccatgctcgc tggccagtgc ctgcaccgtc tcggcctgct cgcgcacacc
     1201 gctgggcgag ccgtggaact ccatcagcag catcggctcc tcgcgcagat ccagcttgct
     1261 gtgggcgttg accatgcgca cgctgttggc gtcgatcagc tccacgcgtg cgatcggcac
     1321 gcccagctgg atcgttgcaa tgacggtgcg caccgcggcc tcgatgctgg ggaaggagca
     1381 gatggccgcg ctcaccgcct cgggcagggg gaacagacgc agggtgacct cggtgaagat
     1441 gcccagcgtg ccctcgctgc ccaccatcag gcgcgtcagg tcgtagccgg cgctgctctt
     1501 cttggcgcgc gtgccggtgc gtatcgcctc gccgctggcc gtcaccacct ccagcgccag
     1561 cacgttctcg cgcatcgtgc cgtagcgcac ggcattcgtg ccgctggcgc gcgtggcggc
     1621 catgccgccc atgctggcat ccgcgcccgg gtcgatgggg aagaacaggc ccctgtcctt
     1681 gatggcctcg ttgagttgct tgcgcgtgat gccgggctgt acggtgaccg tgaggtcatc
     1741 ggcatcgatg ctcagcacgc ggttcatgcg gttcacgtcg atgctgatgc cgccctgcac
     1801 ggccagcaga tgaccttcga gcgaggagcc ggcggcgtag gcgatcaccg gcacggcgtg
     1861 ctgcgcggcc aggcgcacgg catcctgcac gtcctgccgg ctctcggcaa agaccacggc
     1921 ggccggcggt ggtgcttcga tggagccctc gtcgcgtcca tgctgtgcgc gcacggccag
     1981 ggcgctggaa tattgcgtgc caaagcgctg ggacaacgca tccagcaggg cctgaggcac
     2041 ggggcgggca agcagttggg tggtatgggc gggggcgttc atggggctgc gacgggctgg
     2101 atggaagcac ccattctaaa agtcgagcca ccgcccaagg cgcaggcaaa aaaagcccgc
     2161 caaggccctg gagtacagcc ttggcggggg aaggtgcctg gcgcctggcg ctcaggcagg
     2221 gcgcttcaac gaggcttctg cgccgcgggc gctgctcccg atgatgagct tgagcccggt
     2281 gggtgcgggg ctgcggctgg cctggtgctt ggactgcgcg gccggcgtgg atgaggtctg
     2341 ggcctgcgcc aacgcaccgc ccatggtaag ggctgcgaac agcatcacgc tgcgtgcgtc
     2401 gaacggggag atggggcgtc gctgctgtgt tgtcggcatc gattcggtct ccggcaagag
     2461 ttgaacaggg ccgcattgca ccgcagcaaa aaagaccagg ccagcaattt ttcccgctgt
     2521 tgccctactc aacaattggt cggcaggtgt aacgcacaat cgccgtttcg accccgaaac
     2581 tgaaggaatg cgccatggga aacagactga cacagatcgc cacacgcacc ggtgacgacg
     2641 gcaccaccgg cctgggcgac aacacgcgcg tatccaaggc cagcggccgc ccgcacgcca
     2701 tgggggatgt ggatgagctg aattcgcaca tcggcctgct gctgtgcgag agcctgccag
     2761 ccgacatgcg cgagctgctc atcgacgtgc agcaccagct gttcaactta gggggcgagc
     2821 tgtccatgcc cggctatgag ctgctcaagg acgacggcct gctgcagctg gacaacgccc
     2881 tggcgcgcta caacgccgag ctgccgcgcc tgcaggaatt catcctgccc gccggtacgc
     2941 gcgctgcctg ccaggcccat gtctgccgca cggtggcgcg ccgcgccgag cgcgccgtgg
     3001 tggggctgca ggagggcgag gccatgcgcg ctgcgccgcg ccagtacctg aaccgcctct
     3061 cagacctgct gttcgtgctc tcgcgcgtac tcaaccggct cgatgccggc agcgaggtgt
     3121 actggaagag cgagcgcctg gcgcggcaga atgctactga ataaatagct gttgacgctt
     3181 gattgataag cgctgcaggc caattcgcct gcctatgcac atatcgcaga ggcccctcac
     3241 ccctccctct ccccaaaggg gcgagggcga ccggttgcgc cggctttact ccctctccct
     3301 ctgggagagg gttggggtga gggctcgcgg cacagtctga ggtatgtgcc taatcagacc
     3361 aattcgactt gaacctgacg caagctaggc cagcggcgca gccgctcagg gggttgttct
     3421 ttatttcggc gccagcaccg ccatggtgat gcgcgagacg caggtcagct gcccctcgtc
     3481 gttcaccatg tcgatctgcc agacctgggt ggtcttgccg atgtgcaccg gccgcgccgt
     3541 gcccgtgacc cagccgctgg tggcggcgcg caggtggttg gcgttgatgt ccaggcccac
     3601 ggcgcgctgg cctgccgggc aggaatagaa ggcgcccatc gaccccaggg tctcggccag
     3661 caccacgctc acgccgccat gcagcaggcc atagggctgg cgcgtgcgct cgtccaccgg
     3721 cacccggccg cgcagaaagt catcaccaat ctcggtgatc tctatgccca ggtgcgctat
     3781 ggctgtgttg gcatgggcac tgtgcagcag ttcaagggtc aggggttttt tccagatcgg
     3841 catggtgcaa ggcaatgtgg gttgcaaagc ctgcatcatg cccactgtgg ttcgcccctg
     3901 cagcgcttag gcgacagccg ccagctcgtc ctcggtggcg cgcatgctgc cggtctccag
     3961 gcagcgctgg tgccagctaa gcgcctcgct gagcaggtgc ggcgtgtgct gcgcgccctg
     4021 gcggcgcgcg cggtcgaagt agtcctgcag catggggcgc cagtccgggt gggcgcattt
     4081 gtcgatgatg acctgcgcgc gctggcgcgg cgacaggccg cgcaggtcgg ccaggccctg
     4141 ctcggtgacg atgatctgcg tgtcgtgctc ggtgtggtcc acatgcgaga ccatgggcac
     4201 gatgcagctc acgtcgccgc ccttggccac cgagggcgtg acaaagaacg acaggtagcc
     4261 gttgcgcgcg tagtcgcccg agccgcctat gccgttcatc atgccgctgc ccatgatgtg
     4321 cgtgctgttc acgttgccgt agatgtcggc ctcgatcatg gcgttcatgg cgatcacgcc
     4381 caggcggcgc accagctcgg ggtggttgct gatctcctgc gggcgcagca cgatgcgctg
     4441 gcggtaaaag tcaatgttgt cggtgaagtc cttgagcgcc tcgcggctca gggatatggc
     4501 ggtggccgac gccgccgcca tcttgccgcg cttgatcagc tccagcatgc cgtcctgcag
     4561 cacctcggta aagcccagca ggtcctcgaa cgggccgtcc agcagcccgg ccagcacggc
     4621 attggccaca ttgcccacgc ccgactgcag cggcagcatg gccttgggca ggcggcccag
     4681 cttcacctcg tgcgccagaa agtcgatgat gtgcgccgca atctggttgc tgaccgcgtc
     4741 gggccgggca aaggccgtgt cgcggtcgcc gtggtgcgtc tcgaccacgg cgatcacctt
     4801 gtttgggtcc acgcgcagat agggctcgcc gatgcgctct atcggctgcg tcaggtggat
     4861 gggctggcgc cgcggcggaa tggccgtgcc gtagtagatg tcatgcatgc cctccatctc
     4921 cttcgggggc aggctgttga cctccaggat caccttgtcg gcaatatcca gccaggtctt
     4981 gttgttgccc acggcggccg aggggatcag gcgcccgtcg ggcaagatgc ccagcacctc
     5041 caccaccgcc acatccatgc ggcccagaaa gccgaaccag gcctgctgcg ccacatgcga
     5101 caggtggatg tccaggtaat gcagctcgcc ggcgttgatc ttgttgcggc acagcgggtc
     5161 ggactggaaa ggcaggcgct ggtgcatgcc gcccaccttg gccagcgcgc cgtccagctc
     5221 gggcgcggtg gaggcgcccg tccacacatt cacctgaaag ggcttgcccg ccgcatgctc
     5281 ggccgctatg cgctgggcca gggcctgcgg caccagcttg gggtggcccg agccggtgaa
     5341 gccgctcatg ccgacggtgg cgccggccgg aatcagcgcc gcggcatccg acgcgctcat
     5401 gattttctgg gccaggcccg ggtgctggat acgggaagac atcgttgggg acacacacac
     5461 tccagagaaa caatcgcggc gcgcggcatc tgcgggcgcc tgctgccgct ggtaagaaag
     5521 acagcaaatt ttaacagtca tgcaagggtt aacccttgtc tccagagtgt ggcgaatggc
     5581 tgtacggccg ctcatggcat ggccgtgcag gcgctaccat cccccattca tgccatcgac
     5641 ctcactttgc ccctcctgcc gccagccggc cgacaagcgc cgctttgccg ccgccgacgg
     5701 cagcatgctg gagctggatc tgtgctttgc ctgccagggc ctgtggttcg acccgcagga
     5761 gaacacgcgc ctggcgccgg ccgccgtgct gcagctcttc gcgctgctgc atgaacacca
     5821 tggcgacgcg caccaccccc tgtccgaccg gctgcgctgc ccgcattgcc gcaaagacct
     5881 ggtcaagggc tacgacctgg cccagggcgg gcgctacgtc acctaccgct gcgcggcacg
     5941 gcacgggcgc ttctccacct ttggctcctt catggtcgaa aagggctttg tgcgccacct
     6001 ctccacgctc gagatcgagg ccctggcgcg gcgcgtgggc agcatcgcct gcaccagctg
     6061 cgggggcacg gtggacatac gcaacgacca cgcctgcccg tactgccggt cggccctggc
     6121 actgctcgac ccccaggcgg tggacaaggc cctgcagcgc cacggccagg ccgccagtca
     6181 agcagccagc caggcggcca acggccagtc gcccgaggcc ctggccgacg ccctgatcgc
     6241 cctggaacgc aaccgcacgc gcgaggaacg cgagcgccag cgcgaacgtt tcgagggcag
     6301 gcgggaggat cagttcgacc tggtggcagc gggcatagag ttcgtgtggt cgctgctgcg
     6361 gcgctgag
//
LOCUS       DAOMTQ010000169         6290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2910_length_6290_cov_18.450842, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000169 DAOMTQ010000000
VERSION     DAOMTQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2910_length_6290_cov_18.450842"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..2672
                     /locus_tag="PK518_13430"
     CDS             120..2672
                     /locus_tag="PK518_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HRM49957.1"
                     /translation="MGNCRWLRCLLSSGGSHLSVSPVNMSPLFVAHHRPSDNTWQQLD
                     NHLLGVGALAAQFAGKLGLQEMGELLGLLHDLGKYSQEFQTYLKSAIGALNQDEDEDW
                     VDAKSLKGKVDHSTAGAQFAWQALGKAGTLETAQALALCIASHHSGLIDCISARADAF
                     GANLFARRMEKAQQRTHLDEVQRLADPQLLQRCRGLLADVRVASKFQEMLTLIGRSNQ
                     GHGSPVTTQQQWGLLVRFLFSCLIDADRIDTADFEKRRQRSFRPGGRYTDWPVLIERL
                     ERHLALLPPTRPIDHLRQDISLHCQQAASRPVGIYTLTVPTGGGKTLASLRFALHHAR
                     ERGLDRIIYIIPFTSIIDQNARVVRDVLEPADAPQDQGRVVLEHHGSLTPEQQTWREK
                     VLCENWDAPVVYTTMVQLLGALFGAGTRGARRMHQLARAVLVFDEVQTLPVKCAHLFN
                     NAINFLAAQCNTTVLLCTATQPLLDSVNPTLGATRLATAPELMPNVGHLFKALKRVAV
                     NDQRKAGGWSDQEVADLALAEVQASGSCLVIVNTKASARRIFELCATAMEPDLVFHLS
                     TDMCPAHRKARLAALLVRLKADQPTLCVSTQLIEAGVDVDFGSVIRFMAGLDSIAQAA
                     GRCNRNGLRATGSVHVVNPQVESLDKLPDILQGRNAALRVLDEFQQNPERFDGDLLSP
                     AAMHAYYRYYFFDRAADMSYAVAAGDAAQNDTLLNLLSLNTASVTEYARQHASAPPLF
                     FRQAFMTAATAFRAIDSATQAVIVPFGPDGKQLIADLCAAYDVELEIELLRRAQQYSV
                     NVFPHVLKKLMAAQALHEVKPDTRILCLNERYYSDLFGLVTEPVSPMEVLYV"
     gene            2698..3390
                     /gene="cas5c"
                     /locus_tag="PK518_13435"
     CDS             2698..3390
                     /gene="cas5c"
                     /locus_tag="PK518_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HRM49958.1"
                     /translation="MSGRYALFTDPLSKVGGEKCSYHVPTYEALKGIAKSIYWKPTLI
                     WVIDEVRVMKRIRTQTKGVKPLDLGGGNSLAIYTFLADVQYQVRAHFEWNEHRPELTD
                     DRIEAKHHAIAQRMVERGGRQDIFLGTRDCQGYVQACEFGAGAGELDDAGELAFGVMF
                     HGFDYPDETGGTGLHARFWRPTMVNGVIRFARPQDCTERRLVRTMSIKPFGLNQGVRP
                     VDADACEMGVSA"
     gene            3387..5312
                     /gene="cas8c"
                     /locus_tag="PK518_13440"
     CDS             3387..5312
                     /gene="cas8c"
                     /locus_tag="PK518_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HRM49959.1"
                     /translation="MSWLPKLLATYDECKGKEPQGSTPLMPICHSTQNAQIEIVLDGA
                     GQFRRASVLEKAQGVTLIPCTEASGGRSGKKPVNHPLHDKLQYLAQDFTSWDGEVTVG
                     FANDPAEPYRDYVKSLSDWAAADPQPKLLAVLAYVQGGHVVADLIQAGILPVGDDGKL
                     LKTWSADKASAPAIFKVLAPQQSPDDAFVRWRVEVGDDPNSAPWTDEALLASWIAHYQ
                     RSQEKVGLCMATGQHAALAVQHPAKLRHGGDKAKLISANDSSGYTYRGRFTSDEEALG
                     VGFVATQKAHNALRWLIERQGYRSGDQVFVAWEPTGKSIPDPMLATFAAFGGASEDWA
                     LGSSHADAGQAFSLQLRKAIAGYAALLAPRDEVVVLGLDSATPGRMAITFYRELKGSE
                     FLARLKTWHAQFAWPQSYGKECQFIGAPAPRDIAEAAFGSRVDDKLRASTVERLLPCI
                     VDGVPLPLDLLRSAVRRVANRVGMEAWEWERCLGIACALFKGFFNERGYLMALEQERT
                     SRDYLFGRLLAVAEDMESYALYAAGEKARDTSAARLMQRFADRPVSTWRTIELSLRPY
                     IARLRAMRPGVLHKKESLLDDIVALFDPQEFLTDTPLSGEFLLGYHCQRQALRSRGES
                     SDDASDTTPTATTGEPA"
     gene            5309..6151
                     /gene="cas7c"
                     /locus_tag="PK518_13445"
     CDS             5309..6151
                     /gene="cas7c"
                     /locus_tag="PK518_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HRM49960.1"
                     /translation="MSALQNKIDFAVVLRVKRANPNGDPLNGNRPRTDYGGFGEITDV
                     CLKRKLRDRLLESGQAIFVQSDDRKVDGETSLRNRAESAVNGLGKDAFKKNANKDATA
                     KLACAKWFDVRAFGQVFAFAAGGDAGGVSIPIRGPVTIQSAFSVEPVFITSTQITKSV
                     SGEGDGTKRGSDTMGMKHRVDSGIYCTFGSINPQLAERTGFGDADAQELKAILPRMFE
                     NDASSARPDGSMEVLTVVWWRHNCKSGQYSSAKVHGSLTVNPDGSIGLDPLDGLDPEI
                     INGF"
BASE COUNT         1229 a         1877 c         1957 g         1227 t
ORIGIN      
        1 gtgggaggga gcgggatgtc ggaaccttgg gcccattcgg gtgggcattt gctggtggag
       61 catttacggg cggtagccca catggcagcc gtttttttaa gtgtcctgga attccgtctt
      121 tgggaaattg caggtggcta cgatgcctgc tttcttcggg aggcagtcac ttgtcagtgt
      181 caccagtaaa tatgtcaccg ttattcgtcg cccatcatcg cccatcggat aacacgtggc
      241 agcagctgga caatcacctg ctcggtgtcg gagcgctcgc tgcgcagttc gcaggcaaac
      301 ttgggttgca agaaatgggc gaactgctgg gcctgctgca cgacctgggc aagtacagcc
      361 aggaatttca gacgtatctg aaatccgcca tcggtgcact gaaccaggat gaggacgagg
      421 attgggtgga tgccaaaagc ctgaagggca aggtggatca ctcgaccgca ggggcgcagt
      481 ttgcgtggca ggcgctgggc aaggctggaa cactcgaaac ggcccaggcg ctggccttgt
      541 gcatcgcctc gcaccattca ggcttgatcg attgcatcag cgcacgggcg gatgcttttg
      601 gagcaaatct ttttgcccgc cgcatggaga aggcgcagca gcgcacccac ctggacgaag
      661 tgcagcgcct cgccgatccc cagttattac aacgctgccg aggcttgctc gcagatgtcc
      721 gcgtcgcctc caagtttcag gaaatgctca cactgatcgg gcgcagcaac caagggcacg
      781 gcagtccggt gacgacccag cagcaatggg gcttgctggt gcggtttctg ttcagttgcc
      841 tgatcgatgc cgaccgcatc gacacggccg attttgaaaa gcggcgccag cgcagctttc
      901 gccctggcgg gcgctacacc gattggcccg tgttgattga acgcctggag cgccacttgg
      961 cgctgctgcc gcccacccgc cccatcgacc acctgcgcca ggacatttcc ttgcattgcc
     1021 aacaagctgc ctctcggccc gtcggcatct acaccctgac cgtgcccacg ggtggcggca
     1081 aaaccctggc cagcctgcgc tttgccttgc accacgccag ggaacgtggc ctggatcgga
     1141 tcatctacat catccccttc acgtccatca tcgaccagaa cgccagggtg gtgcgagacg
     1201 tgctggagcc tgcggatgcc ccgcaagatc agggccgtgt cgtgctggaa caccacggca
     1261 gcctcacgcc agagcagcag acctggcgcg agaaggtgtt gtgcgagaac tgggatgcac
     1321 cggttgtcta caccaccatg gtgcagttgc tgggagcctt gtttggcgcg ggtacccgtg
     1381 gcgcacgccg tatgcaccag ctggcccggg cggtgctggt gttcgacgag gtgcaaaccc
     1441 tgcccgtgaa atgcgcccac ctgttcaata acgccatcaa ttttttggcg gcgcagtgca
     1501 acaccacggt gctgctgtgt accgccacgc agccgctgct cgatagcgtc aatcccactt
     1561 tgggcgctac gcgtttggcg acggcccctg agttgatgcc caacgtcgga catctgttca
     1621 aggcactcaa gcgcgttgcg gtgaacgatc agcgcaaagc cggtggctgg agcgatcagg
     1681 aagtggccga tctcgccttg gccgaggtac aggccagcgg gagttgcctg gtcatcgtca
     1741 ataccaaggc gtcggcccgg cgtatttttg agctgtgcgc caccgcgatg gagccagacc
     1801 ttgtgtttca tctgagcact gacatgtgcc cggcccatcg caaggcacgt ctggcagcac
     1861 tgcttgtgcg tttgaaggca gaccagccga cgctgtgcgt gagcacgcaa ttgatcgagg
     1921 ccggcgtgga tgtggacttt ggcagcgtca tccgtttcat ggccgggctc gactcgattg
     1981 cccaggcggc ggggcgctgc aaccgcaacg gcctgcgtgc caccggcagc gtgcatgtcg
     2041 tcaacccgca ggtggaaagc ctggacaagc tacctgacat cctgcaaggc cgcaacgccg
     2101 ccttgcgggt gctcgatgag ttccagcaaa acccggagag attcgatggt gacttgctca
     2161 gccccgcggc aatgcacgcc tactaccgct actacttctt cgatcgtgcc gcagacatga
     2221 gctatgcggt ggccgctggc gatgccgcgc aaaacgacac cttgctcaat cttttgtcgc
     2281 tgaataccgc cagcgtgacc gaatacgccc gccagcacgc atcggcaccc ccgctttttt
     2341 tccgccaggc cttcatgaca gcggccacgg ccttccgtgc catcgactcg gccacgcagg
     2401 cggtcatcgt tccctttggg cctgatggca agcaactgat tgccgacctg tgcgcggcct
     2461 acgacgtgga gctggaaatc gagctgctgc ggcgggcgca gcaatacagc gtgaacgtgt
     2521 ttccccatgt gctgaaaaaa ctcatggcgg cgcaagcctt gcacgaggtc aagccggaca
     2581 cacgcatcct ttgtttgaat gagcgctact acagcgactt atttggtctg gtcactgagc
     2641 ctgtttcccc catggaggtt ttgtatgtct aagccgcgac gcagcatcga gttcgaggtg
     2701 tccgggcgct acgccctgtt caccgaccct ttgagcaagg tgggtggcga aaaatgctcg
     2761 taccacgtcc cgacctacga agccttgaag ggcatcgcca aatccattta ctggaagcca
     2821 acgctgatct gggtgatcga cgaagtgcgg gtgatgaagc gcatccgcac ccagaccaag
     2881 ggagtcaagc cgttggatct gggtggcggt aacagcctgg ccatctacac cttcctggcc
     2941 gacgtgcaat accaggtgcg agcccacttt gagtggaacg agcaccgccc cgagttgacg
     3001 gatgaccgca tcgaagccaa gcaccacgcc atcgcccagc gcatggtcga acgcggcggg
     3061 cggcaggata tctttttggg cacgcgcgac tgccaaggct atgtgcaggc atgtgagttt
     3121 ggcgcgggcg cgggagagct ggatgatgcc ggcgaactgg cctttggcgt gatgttccat
     3181 ggctttgact accccgatga aacaggcggc acaggactgc acgcccgctt ctggcgccca
     3241 accatggtta atggcgtgat tcgatttgca cgcccgcagg actgcaccga acgccggctc
     3301 gtgcgcacca tgagcatcaa acccttcgga ttgaatcaag gcgtccggcc cgtggatgca
     3361 gacgcctgcg aaatgggggt gtctgcatga gttggttgcc caaacttctt gccacctacg
     3421 acgagtgcaa gggcaaggag ccgcagggct caacaccttt gatgcccatc tgccacagta
     3481 cgcagaacgc gcagattgaa atcgtgctcg atggtgctgg tcagttcagg cgcgcatccg
     3541 tgttggaaaa agcccagggc gtgaccttga tcccttgcac cgaggcatcg ggcgggcgca
     3601 gtggcaagaa gcccgtcaac cacccgctgc atgacaagct gcaatacctc gcccaggact
     3661 tcacgtcatg ggatggggag gtgacggtcg gtttcgccaa cgaccctgcc gagccgtatc
     3721 gcgattacgt gaaatctctg tcggattggg ccgctgccga tccacagcca aagctgctgg
     3781 ccgtactggc ctatgtgcag ggtggtcatg tggtggccga cttgattcaa gcaggcattc
     3841 ttcctgtggg cgacgacggc aagctgctca agacgtggag tgctgacaag gccagcgccc
     3901 ccgccatctt caaagtcttg gcaccgcagc aatcgcccga cgatgctttt gtgcgctggc
     3961 gcgtggaggt gggcgacgac cccaattcag cgccatggac agatgaagcg ctgctggcct
     4021 cctggattgc gcactaccag cgcagccagg aaaaagtggg cttgtgcatg gcgacgggcc
     4081 agcacgctgc attggccgtg cagcacccgg cgaagctgcg ccacggtggc gacaaggcga
     4141 agctgatttc cgccaatgac agctcgggct atacctaccg ggggcgcttc accagcgacg
     4201 aagaagcctt gggcgttggc ttcgttgcca cccaaaaggc gcacaacgct ctgcgttggc
     4261 tgattgaacg gcagggctac cgcagcggcg atcaggtctt tgtagcctgg gagcccacgg
     4321 gtaaatcgat tcccgacccc atgttggcca cgtttgcggc gtttggcggt gcatccgaag
     4381 attgggcgct gggcagcagc catgccgatg ccggacaggc tttcagcttg cagctgcgta
     4441 aggccattgc gggctacgcc gcactgcttg cgcccaggga tgaagtggtg gtgctggggc
     4501 tggattccgc aacccctggg cgcatggcca tcacctttta ccgtgaactc aagggctccg
     4561 agtttctggc acgcctgaaa acgtggcacg cgcaatttgc ttggccgcag agctatggca
     4621 aggagtgcca attcattggt gcaccggcac cgcgcgacat tgcggaagcg gcctttggca
     4681 gccgcgtgga cgataagttg cgcgccagta ccgtggagcg gctgctgcct tgcatcgtcg
     4741 atggcgtgcc gctgcccctg gatctgcttc gatcagcggt gcgccgcgtg gccaatcggg
     4801 tcggaatgga agcgtgggaa tgggagcgct gcctgggcat tgcctgcgca ctgttcaaag
     4861 gttttttcaa cgagaggggg tatctgatgg cattggagca agaacgcacc agccgcgact
     4921 atttgtttgg tcgcttgctg gcggttgcgg aagacatgga gagctacgcg ctctacgccg
     4981 caggcgagaa ggccagggac acctcagcgg cgcgcctgat gcagcgcttt gcagatcggc
     5041 cggtttcaac gtggcgcacc attgaattgt ccttgcgccc ctacatcgcc aggctgcgtg
     5101 cgatgcgccc aggggtgttg cacaagaaag agagcctgct cgatgacatc gtggcgctgt
     5161 ttgatccgca ggaatttttg accgacacgc cactgagcgg cgaatttttg ctgggctacc
     5221 actgccaacg tcaagccttg cgctcacgcg gtgagtcaag cgatgacgca tccgatacca
     5281 ccccaacagc tactactgga gaacccgcat gagcgctttg caaaacaaga tcgacttcgc
     5341 cgtggtgctg cgcgtcaaac gcgccaaccc caatggcgac cccctgaacg gcaaccgccc
     5401 gcgcaccgac tacggcggct ttggcgagat caccgatgtg tgcctcaagc gcaagctgcg
     5461 cgaccgcctg ctggagtctg gccaggccat tttcgttcag tcggacgacc gcaaggtgga
     5521 tggcgaaacc agcctgcgca accgcgcgga gtccgctgtc aacggtttgg gcaaggacgc
     5581 cttcaagaag aacgccaata aggatgcaac cgccaagttg gcctgcgcca agtggtttga
     5641 cgtgcgggct ttcggccagg tgtttgcttt tgctgctgga ggtgatgcag gcggggtgtc
     5701 catccccatc cgtggcccgg tcaccatcca gtcggccttt agcgtggagc cggttttcat
     5761 caccagcacg caaatcacca agagtgtcag cggtgagggc gatggtacga agcgcggttc
     5821 agacaccatg ggcatgaagc accgcgtgga cagcggtatc tattgcacct ttggcagcat
     5881 caacccgcaa ctggccgagc gaacggggtt tggcgatgcc gatgcacagg agctcaaagc
     5941 catcctgccc aggatgttcg agaacgacgc ttcctcagcc cggcccgacg gcagcatgga
     6001 agtgctgacc gtcgtctggt ggcggcacaa ctgcaaatcc gggcagtatt cgtcggccaa
     6061 ggtgcatgga tccttgacgg tgaaccccga tggcagcatc ggtttggatc ctttggatgg
     6121 gctggatcca gaaatcatca atggcttttg acgaaagcgc tgcaaggcct gctgcaactg
     6181 ccatgcaggc cttttcgagg agcatttgat gaaaatcctg cccacccaat tcgatggcca
     6241 cgccatccgt cggctgtatg acgaggcgac ggacacctgg tggttttccg
//
LOCUS       DAOMTQ010000170         6225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_2954_length_6225_cov_21.166937, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000170 DAOMTQ010000000
VERSION     DAOMTQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_6225_cov_21.166937"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..821
                     /locus_tag="PK518_13450"
                     /pseudo
     CDS             234..821
                     /locus_tag="PK518_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
     gene            833..2203
                     /locus_tag="PK518_13455"
     CDS             833..2203
                     /locus_tag="PK518_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM49961.1"
                     /translation="MNKQTQAAAQAASSTDLQTLLGDGLARRWWQSITLWLGLLAVAA
                     VAGGWWYWQSSQQAQAAPRYVTQEVQRGQVKLTVAANGTLQPTRSVTIGSELSGTVRR
                     VLVDVNDQVKKGQVLVELDTTKLDSQVLRSRASVASAQARLAQATATTKEARASLARL
                     EEVARLSGGKVPSAAELDAGRAAYERAQADEAAARASVQDARAALATDETNVTKASIR
                     SPIDGVVLSRVVEPGNAVAASLQAVTLLTLAEDLGRLRLEVNVDEADVGSVQPGQQAS
                     FTVSAYPSRPYPARTTRVAFGSTKTDNVVTYITYLEVDNADLSLRPGMTAAATITSTE
                     RNDVLTVPNTALRFTPMGAAGDGAAQGAKPAAGGDKAGGGSGIMGQLMPRMPRPTGQR
                     RSAADGNGKAGAGQRQIWVLEAGSPKAVRVQTGISDGRVTEVHSDALQPGMQVITDQL
                     AAGAKP"
     gene            2200..2973
                     /locus_tag="PK518_13460"
     CDS             2200..2973
                     /locus_tag="PK518_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM49962.1"
                     /translation="MSAQAQPSQDSQPLIRLRGITKVYGEGALAFQALKGVDLDIAAG
                     EFLAIMGPSGSGKSTAMNVIGCLDRPSAGDYLFRGVPVQALSRDDRARLRRRFMGFVF
                     QGFNLLARTSAQENVELPLLYRGESAAARHAAAARALQAVGLTGWEHHTPAELSGGQQ
                     QRVAIARAMVSQPAVLLADEPTGNLDTQRSREIMELLWRLNVDQGITVLMVTHEMEMA
                     QYARRIVRFVDGRIDSDRANPEPYALARAAAAQPAEVAP"
     gene            2973..4181
                     /locus_tag="PK518_13465"
     CDS             2973..4181
                     /locus_tag="PK518_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM49963.1"
                     /translation="MLLNSLLLALRSIRRNLMRSFLTVLGIVIGVSAVITMVTLGNGA
                     TLSVQAQIASLGTNLLQVRPGQRMGPGGASGAPAFKDVDADAIAQQIGGILAVAPEAR
                     TSATAVAGGRNWTTSVLGSTNDWLQTGSWTLARGREFTPDEQRVGAAVCLIGQTVQRE
                     LFGASTDPLGERVRVRQFSCEVVGVLASKGQGAFGNDQDDVLLLPLKTLQRRVIGSTR
                     VPTLLVSMKDGSDAERVKASLTELLRERRKLSDSDDDNFNVLDTKQLADTFSGTTRVM
                     TTLLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGLRLAIGALEREVLMQFLIEAVVL
                     AALGGLIGIVLATGASMLLAGAMGIPYIFHPGVNVLAFVFSAVIGVVFGFFPARRAAR
                     LDPIEALRHE"
     gene            4331..5119
                     /locus_tag="PK518_13470"
     CDS             4331..5119
                     /locus_tag="PK518_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HRM49964.1"
                     /translation="MIASLISSPRLFLAVCTGLLASALAGCARLPEAMPGLEQRHLQF
                     TPNDFDAASMHTRHVLDSQANTCEAARRALLSQGYLVNTASADTVAGRKYYQPHQDVH
                     YEVQMRVVCAPEGQGDARTSAFASALQDRYVIKKVNNSASLGVGGLGSVSLPVSATDD
                     TLVKVGSETITDAQFYARFFQLFERYVRLAPPGTEPALMHPASTPAQAPVLAPAPATT
                     AGVVQPAASSQPLPLQEPEAEQRAAATALPPDAAMPPAAPVGQQ"
     gene            5138..>6225
                     /locus_tag="PK518_13475"
     CDS             5138..>6225
                     /locus_tag="PK518_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM49965.1"
                     /translation="MPSLSRRQAVRACSLALLPLSATTTSAQGAPTKLALIESLSGSF
                     ANTGEAVFRNLLWATERVNARGGVKLPGGARPLQLLRYDSKGSNEEALSALRAAIDDG
                     VHFVLQGNSSATAAALIEAISKHNQREPGRRVVFLNYSAVDPLLTNERCNFWHFRFDA
                     HADMRMAALMEVLREDKALQRAYLIGQDYSFGQAVLREARRQLSAQRPDVTIVGDELH
                     PVGRVKDFAPYAVKIKASGAQAVITGNWGNDLTLLVKAAREVGYDGMFYTFYGNALGA
                     PAALGEAGVGKVVAVADWLPNLPTEGSAAFYRAFRERFPKPQDDYVHMRMQLMVESLA
                     QAMEKAASTDALPVARALEGASVSLYGQA"
BASE COUNT          934 a         2192 c         2209 g          890 t
ORIGIN      
        1 ggcggaagcc gcgaagcagc gcctctgtcg cgccacaccc gttcgcggct tccgccgctc
       61 ctgcaacagg cgggcgatga aggatcgcgt ttcctacact gcggcttttc ccgtacacct
      121 tcatgtcaag gagcttcgcc atgcccatgt tcatcgacac ctcgccaccc ggccagtgca
      181 tcttctgccg cctcgtggcg ggcgagatcc cttcggcccg cgtggccgag gacgatctgg
      241 cgctggcctt tccggccgat accctgcgcc agcgccccga tgtgcgccag gccgagcagc
      301 gcgtgcgcgc cgcctgggag cgcgtggccc aggccgagga ccagcgcctg cccagcctgc
      361 gcattggtgg cacgctgggc ctgcgcgcgc tgaccctggg ggcgctcacg ggcggcggcg
      421 ccacggcggc atcgctgctg gccagcgtgt cgttgccggt gtttgatggt ggcgcgctgc
      481 gcgcccaggt gcgcgcgcag caggccgcgc tggagcaaag ccgcgcgggc tatgaagagg
      541 cggtgctggg cgcgctcaag gatgtggagg acgcgctggt ggcgctgcag ggcgagcgcg
      601 agcggctgac gcgcctgaac gccgcggccg atgccgcggg caacgcggcc ctgctggcgc
      661 agcagcagta cgccagcggt ctggtggact ttcagaccgt gctggacacg cagcgccagc
      721 tgctctcggc ccaggacggc gtggccagca ccaccgccag cataggcacg ggccatgtgc
      781 gcctgtacaa ggcattgggc ggcggctggc aaagcaattg acagggacag gaatgaacaa
      841 gcaaacacaa gcagcggcgc aggccgcctc gtccaccgat ctgcagaccc tgctgggcga
      901 cggcctggcc cggcgctggt ggcagagcat tacgctgtgg ctgggcctgc tggccgtggc
      961 cgccgtggcc ggtggctggt ggtactggca gtccagccaa caggcccagg ccgcgccgcg
     1021 ctatgtgacg caggaggtgc agcgcggcca ggtcaaactg accgtggcgg ccaacggcac
     1081 gctgcagccc acgcgctcgg tgaccattgg cagcgagctg tcgggcacgg tgcgccgcgt
     1141 gctggtggat gtgaacgacc aggtgaagaa gggccaggtg ctggtggagc tggataccac
     1201 caagcttgac tcccaggtgc tgcgctcgcg tgcctcggtg gcctcggccc aggccaggct
     1261 ggcgcaggct acggccacca ccaaggaggc gcgcgccagc ctggcgcggc tggaagaggt
     1321 ggcgcgcctg tctggcggca aggtgccctc ggccgccgag ctggacgccg gccgcgccgc
     1381 ctacgagcgc gcccaggccg acgaggccgc cgcccgcgcc agcgtgcagg acgccagggc
     1441 ggcgctggcc accgatgaga ccaacgtcac caaggcctcg atccgctcgc ccatcgacgg
     1501 cgtggtgctg tcgcgcgtgg tcgagccggg caatgccgtg gccgcctcgc tgcaggccgt
     1561 gaccctgctc acgctggccg aagacctggg ccggctgcgc ctggaggtga acgtggacga
     1621 ggccgacgtg ggctcggtgc agccgggcca gcaggccagc ttcaccgtca gcgcctaccc
     1681 ctcgcgccct tacccggcgc gcaccacgcg cgtggccttc ggctccacca agaccgacaa
     1741 cgtggtcacc tacatcacct acctggaggt ggacaacgcc gacctgagcc tgcgaccggg
     1801 catgacggcg gcggccacca tcacctccac cgagcgcaat gacgtgctca ccgtgcccaa
     1861 cacggcgctg cgcttcacgc ccatgggcgc ggccggcgac ggcgccgccc agggcgccaa
     1921 gccggccgcc ggcggcgaca aggcgggcgg cggcagcggc atcatgggcc agctgatgcc
     1981 gcgcatgccg cgccccacgg gccagcgccg cagcgccgcc gacggcaatg gcaaggcggg
     2041 cgccgggcag cgccagatct gggtgctgga ggcaggcagc ccgaaggccg tgcgcgtgca
     2101 gaccggcatc agcgacggcc gtgtgaccga ggtacacagc gacgcgctgc agcctggcat
     2161 gcaggtcatc accgaccagc tggcggcggg ggccaagcca tgagcgccca ggcgcagccg
     2221 tcgcaagaca gccagccgct gatccgcctg cgcggcatca ccaaggtcta tggcgagggc
     2281 gcgctggcct ttcaggcgct caagggcgtg gatctggaca tagcggccgg cgagttcctg
     2341 gccatcatgg gccccagcgg ctcgggcaag tccacggcca tgaacgtcat cggctgcctg
     2401 gaccggccca gcgccggtga ctacctgttt cgcggcgtgc cggtgcaggc cctgtcgcgc
     2461 gacgaccggg ccaggctgcg ccggcgcttc atgggctttg tgttccaggg cttcaacctg
     2521 ctggcgcgca cctcggcgca ggagaacgtg gagctgccgc tgctctaccg cggcgagagc
     2581 gcggccgcgc gccacgcggc cgcggcccgg gcgcttcagg ctgtgggcct cacgggctgg
     2641 gagcaccata cgccggccga gctctcgggc ggccagcagc agcgggtggc gattgcacgc
     2701 gccatggtca gccagccggc ggtgctgctg gccgacgagc ccacgggcaa cctcgacacc
     2761 cagcgcagcc gcgagatcat ggagctgctc tggcgcctga acgtcgatca gggcatcacc
     2821 gtgctgatgg tcacgcacga gatggagatg gcgcagtacg cgcggcgcat cgtgcgcttt
     2881 gtcgatggcc gcatcgacag cgaccgggcc aaccccgagc cctacgcgct ggcgcgcgcg
     2941 gcggcggcgc agccggcgga ggtggcgcca tgatgctgct caactcgctg ctgctggcgc
     3001 tgcgctcgat acggcgcaac ctgatgcgct cgtttctcac cgtgctgggc atcgtcatcg
     3061 gcgtcagcgc ggtgatcacc atggtcacgc tgggcaacgg cgccacgctg tcggtgcagg
     3121 cgcagattgc cagcctgggc accaatttgc tgcaggtgcg cccgggccag cgcatggggc
     3181 cgggcggcgc cagcggcgcg ccggccttca aggacgtgga cgccgacgcc atcgcccagc
     3241 agatcggcgg cattctggcc gtcgcgcccg aggcgcgcac cagcgccacc gccgtcgccg
     3301 gcgggcgcaa ctggaccacc agcgtgctgg gcagcaccaa tgactggctg cagaccggca
     3361 gctggacgct ggcgcgcggc cgcgagttca cgcccgacga gcagcgcgtg ggcgcggccg
     3421 tgtgcctgat cggccagacc gtgcagcgcg agctgttcgg cgccagcacc gacccgctgg
     3481 gcgagcgcgt gcgcgtgcgc cagttctcct gcgaggtggt gggcgtgctg gcctccaagg
     3541 ggcagggggc gtttggcaac gaccaggacg atgtgctgct gctgccgctc aagaccctgc
     3601 agcggcgcgt gatcggcagc acgcgcgtgc ccacgctgct ggtgtcgatg aaggacggca
     3661 gcgacgccga gcgcgtcaag gccagcctga ccgagctgct gcgcgagcgg cgcaagctct
     3721 cggacagcga cgacgacaac ttcaacgtgc tcgacaccaa gcagctggcc gacaccttct
     3781 cgggcaccac gcgcgtcatg acgacgctct tgggcgcggt ggccgcggtg agcctgctgg
     3841 tgggcggcat aggcatcatg aacatcatgc tggtgagcgt caccgagcgc acgcgcgaga
     3901 tcggcctgcg cctggcgatc ggcgcgctcg agcgcgaggt gctgatgcag tttttgatag
     3961 aggccgtggt gctggcggcg ctgggcgggc tgataggcat cgtgctggcc acgggcgcct
     4021 cgatgctgct ggccggggcc atgggcattc cctacatctt ccatccgggg gtgaatgtgc
     4081 tggcgttcgt gttctcggcc gtgatcggcg tggtgttcgg cttctttccc gcgcggcgcg
     4141 cggcccggct cgatcccatc gaggcgctgc ggcatgagtg agggttgaag gaaaattggc
     4201 cgtttacgct ttatggataa gcgcaaatag ctattttaat tatagcaaaa tcgatgctgc
     4261 caagggcctt cggcgggcca cggccccggc cggcctgcac aatgcgggtt gcactgccga
     4321 ctgtttcgcc gtgattgctt ccctgatttc atccccacgc ctgttccttg ccgtttgcac
     4381 cggcctgctg gccagcgccc tggcgggctg cgcgcgcctg cccgaggcca tgcccgggct
     4441 ggagcagcgc cacctgcagt tcaccccgaa cgatttcgat gccgccagca tgcacacgcg
     4501 ccatgtgctc gattcgcagg ccaacacctg cgaggccgcg cgccgcgcgc tgctcagcca
     4561 gggttacctg gtgaacaccg ccagcgccga cacggtggcc gggcgcaagt actaccagcc
     4621 gcaccaggac gtgcactacg aggtacagat gcgcgtggtc tgcgcgcccg aggggcaggg
     4681 cgatgcgcgc acctcggcct tcgccagtgc gctgcaggac cgctacgtga tcaagaaggt
     4741 gaacaactct gcctcgctgg gcgtgggcgg gctgggctcg gtgtcgctgc cggtgtcggc
     4801 caccgacgac accctggtca aggtcggcag cgagacgatc accgacgcgc agttctacgc
     4861 ccgcttcttc cagctgttcg agcgctatgt gcgcctggcg ccgccgggca ccgagccggc
     4921 gctgatgcac ccggccagca ccccggcgca ggcgccggtg ctggcgccag ccccggccac
     4981 caccgccggt gtggtgcaac cggcagccag ttcccagccg ctgccgctgc aagagcccga
     5041 ggccgagcag cgtgccgcag ccacggcgtt gccgccggat gcggccatgc cgcccgctgc
     5101 gcccgtcggg cagcagtaat ttttctcagg ttcttccatg ccctccctat cccgtcgcca
     5161 ggccgtgcgc gcctgttcac tggcgctgct gccgctgtct gccaccacca ccagcgccca
     5221 gggcgcgccc accaagctgg cgctgatcga aagcctctcg ggcagctttg ccaacaccgg
     5281 cgaggcggtg ttccgcaacc tgctctgggc caccgagcgc gtgaacgcgc gcggcggcgt
     5341 caagctgccg ggcggtgcca ggccgctgca actgctgcgc tacgacagca agggcagcaa
     5401 cgaggaggcg ctatccgccc tgcgcgcggc catcgacgat ggcgtgcact tcgtgctgca
     5461 gggcaacagc tcggccacgg cggcggcgct gatcgaggct atcagcaaac acaaccagcg
     5521 cgaaccgggc cggcgcgtgg tcttcctcaa ctactcggcc gtcgatccgc tgctgaccaa
     5581 cgaacgctgc aacttctggc atttccgctt cgacgcccat gccgacatgc gcatggccgc
     5641 gctgatggag gtgctgcgcg aggacaaggc tttgcagcgc gcctacctga taggccagga
     5701 ctacagcttc ggccaggccg tgctgcgcga ggcgcggcgc cagctctccg cgcagcggcc
     5761 ggacgtcacc atcgtcggcg acgagctgca ccccgtcggc cgggtgaagg acttcgcccc
     5821 ctacgccgtg aagatcaagg ccagcggcgc ccaggccgtc atcaccggca actggggcaa
     5881 cgacctgacg ctgctggtca aggccgcgcg cgaggtgggc tacgacggca tgttctacac
     5941 cttctacggc aacgccctgg gcgcgcccgc cgccctgggc gaggccggcg tgggcaaggt
     6001 ggtggccgtg gccgactggt tgccaaacct gcccaccgag ggcagtgccg ccttctaccg
     6061 cgcgtttcgc gagcgcttcc ccaagccgca ggacgactac gtgcacatgc gcatgcagct
     6121 catggtcgag tccctggccc aggccatgga aaaggctgcc agcaccgacg cgctgcccgt
     6181 ggcgcgcgcg ctggagggcg ccagcgtcag cctgtacggc caggc
//
LOCUS       DAOMTQ010000171         6143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3018_length_6143_cov_25.700558, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000171 DAOMTQ010000000
VERSION     DAOMTQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3018_length_6143_cov_25.700558"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..988)
                     /locus_tag="PK518_13480"
     CDS             complement(68..988)
                     /locus_tag="PK518_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM49966.1"
                     /translation="MHNRHDPRVLPMRDGVNPSCVVLPSIVHGLLLDFLVQRLPAVSR
                     ADWLRRLGAGEVVDEQGVAATAQSPCTPGLRYYYYRELPSETPIPFEETVLYQDEHIL
                     VADKPHFLPVVPAGRYLQQTLLVRLKRRLGLPELSPVHRIDRDTAGLVLFSVQRATRG
                     MYQALFRERAVRKVYEAVAPWRADLPLPRVHESRLEELGHFFRMQEVPGTPNAITAMA
                     VVEQEGPWARYRLEPITGKRHQLRVHMAALGLPLCGDGFYPEVNDPPEGDYSNPLQLL
                     ARTLSFTDPVTGQARDFASRLQLRALASLG"
     gene            complement(990..1820)
                     /locus_tag="PK518_13485"
     CDS             complement(990..1820)
                     /locus_tag="PK518_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM49967.1"
                     /translation="MFRTRPPAYALACAALWFIQLCANLLLVFTNLPSGASHYWLLFN
                     LVTTILLGVLILLQLPRALQWRPRRSARPSHELRQERQRIARDLHDQVGSQLVHAMAM
                     VDAHNPSMQPLAKALEHCLLDVRLLVDSMDGDDDALIDRLARLRHRIQPSLNQRGITL
                     QWDVAFAGAAAMPMGAPARELTAIVQEAASNVLQHSGATAVAIALQHVQDGAGGAWQL
                     QVSDNGKGLPAAALNGQDAGHGLAGMRQRAAKAGGRLQLLPGLQGQGLCIQVTVPTRN
                     "
     gene            complement(1953..2672)
                     /locus_tag="PK518_13490"
     CDS             complement(1953..2672)
                     /locus_tag="PK518_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM49968.1"
                     /translation="MWPDFMTGQVEKPVRVLLVDDDPHMRRVIAQELMADERTMLVAQ
                     AGSVREGRKAIKQHEFDVLLVDLNLGDGEGFELIDYLKSNRPSAEAIVISIMENDDQV
                     LHAFELGATGYLVKNSWFGNYPQAVLQVANGGASITPNLARRLLQRFDHGAPAAPAAA
                     APEAQAPVQATAEHEPERLSQREKEVLRMVASGYTSAEIGTQLQISALTVNTHIKNIY
                     RKLQVRTRAQAVRFASLRGLF"
     gene            complement(2962..6135)
                     /locus_tag="PK518_13495"
     CDS             complement(2962..6135)
                     /locus_tag="PK518_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRM49969.1"
                     /translation="MLPDYAELHALSNFSFQRGASHPQELVERAHALGYSALALTDEC
                     SVAGVVRAHVAARQLGLPLIVGSEFLWGELRIVALARDLQGWGDLCEFISAARACAAK
                     GGYRVDAQSPWALLRGCELLLAPRREVFTDASDLIAISACLESARGLFCYKCWLAVEL
                     HLALDDGLWLGTLTQAGARLGLPLVAAGDVHLHARAAKPLQDVITAVGLGRSLADCGF
                     ALQPHAEKRLRPRSQLAGLYPPELLAATLTVAARCRFSLDEISYRYPLETVLPGMTAQ
                     QTLAWLTWEGAHARYPAGVPERVQAQIGKELDLIADCGYEMYFLTVHDIVRYARSQGI
                     LCQGRGSAANSVVCYCLGITAVDPQHAHLLFERFISKERSEPPDIDVDFEHERREVVI
                     QYIYAKYGRERAALTAVVISYRTRSALRDVGKALGLAPALVDAFAKDHHWFDDVLAVD
                     HLQALAASLGEDLPAHQAALWLELTRRLRGFPRHLSQHVGGFVLTQGKLTRLVPVEPA
                     AMPGRSIIQWDKDDLDAMGLLKVDVLALGMLSALRRCLDARALLRGEPWGLADIPQED
                     PATYDMVCRADTVGVFQIESRAQMSMLPRLQPRSFYDLVVQVAIVRPGPIQGGMVHPY
                     LRARERQRWLGPGEPFPLEYPQLAEALARTLGVPIFQEQVMQVAIAGAGFTPGEADAL
                     RRSMAAWKRVGGVHQFEERFKRGLLLRGYPQEFADRLFQQILGFGEYGFPESHAYSFA
                     LLAYASSWLKRHEPACFLAALLNSQPMGFYAPSQLVQDARRHGVRVLPIAVTASDWDC
                     TLEEPLAPVHGVQLPQPAVRLGLRLVAGLRQDAARRLVHARHDGPFASTEDLALRARL
                     DRQDLQALAAADALRALSGHRRQQMWDAAAQLHALPLLQQAPVHEEALQLPAAPEGED
                     ILFDYAATGLTLRRHPLALLRAHLARWRIATALQLRDIPRGRRVRACGIVTVRQRPGT
                     AQGTMFVTLEDETGPVNVIVWPAQLERWRDALMRARLLAVEGVWQCSDAPDAGMAVRH
                     LIGQRFKDLSPLLGRLAQALGGSRDFH"
BASE COUNT         1038 a         2060 c         2075 g          970 t
ORIGIN      
        1 ttcccttcac ccgctccaca gcaccttcct gcctttgtct gcagggcccc ccctctccca
       61 aaccggctca gccaagactc gccagtgcac gcagctgcag gcggcttgca aaatcgcgcg
      121 cctggcccgt cactggatct gtgaatgaca gggtgcgcgc cagcagctgc agcgggttcg
      181 aataatcacc ttcgggcggg tcgttcacct caggatagaa gccatcaccg cacaggggca
      241 ggcccagcgc cgccatgtgc acgcgcagct ggtggcgctt gccggtgatg ggctccaggc
      301 gatagcgggc ccacggcccc tcctgctcca cgacggccat ggcagtgatg gcgttgggcg
      361 tgcccggcac ctcttgcatg cgaaagaaat ggccgagctc ctccaggcga ctttcgtgca
      421 cgcgtggcag cggcaggtct gcccgccagg gggcgacggc ctcgtacacc ttgcgcaccg
      481 cgcgctcccg gaacaaggcc tgatacatgc cacgcgtggc ccgctgcacc gagaacagca
      541 ccagccctgc ggtatcgcgg tcgatgcggt gcacgggcga gagttcgggc aggcccaggc
      601 ggcgcttcag gcgcaccaac agggtttgct gcaggtagcg gccggcgggt accaccggca
      661 ggaaatgcgg tttgtcggct accaggatgt gttcgtcctg gtagagcacc gtctcttcaa
      721 acgggatggg tgtttcgctg ggcagttcgc gatagtagta gtagcgcaaa cccggcgtgc
      781 aggggctttg cgcggtggcg gcaacgcctt gctcgtcaac cacttcgccg gcaccaagcc
      841 ggcgcagcca atctgcgcgc gagacggctg gcaggcgctg caccagaaag tccagcaaca
      901 gcccatgcac gatcgaaggc agcaccacgc aactgggatt cacgccatcg cgcatgggca
      961 gcacgcgggg gtcgtgccgg ttgtgcatgt caattccgag tgggcacggt cacctgtatg
     1021 caaagcccct ggccctgcag gccgggcagc agctgcagcc gcccgccggc cttggcggcg
     1081 cgctggcgca tgccggccag gccatggccg gcgtcctgtc cgttaagcgc cgcggccgga
     1141 aggcccttgc cgttgtcact gacctgcagc tgccaggcgc cgcctgcgcc atcctgcaca
     1201 tgctgcagcg ctatggccac ggccgtggca cccgaatgct gcagcacgtt gctcgcggcc
     1261 tcttgcacga tggcggtgag ttcgcgcgcc ggcgcgccca tgggcatggc ggcagcgccc
     1321 gcgaaggcca cgtcccactg cagcgtgatg ccacgctggt tcagcgatgg ctgaatgcgg
     1381 tggcgcaggc gggccagtcg gtcgatgaga gcgtcgtcat ccccgtccat ggaatcgacc
     1441 agcaggcgca cgtccagcag gcagtgctcc agcgccttgg ccagcggctg catgcttgga
     1501 ttgtgtgcat ccaccatggc catggcatgt accagctgcg agccgacctg atcatgcaga
     1561 tcgcgcgcaa tgcgctggcg ctcctgcctg agctcatggc tgggacgcgc gctgcgccgg
     1621 ggccgccatt gcagtgcccg cggcagctgc agcaggatca gcacgcccag cagaatggtc
     1681 gtcaccagat tgaaaagcag ccagtagtga cttgcacccg aaggcaggtt ggtgaagacc
     1741 aggagcaggt tggcacacag ctgtatgaac caaagcgcgg cgcaggccaa tgcatacgcc
     1801 ggcgggcggg tgcgaaacat gatgtccctc taatctatgc acccacaagg cacccgcgcc
     1861 ttgtaactgc cttgaccgcg aaagttggcc acaaaatgca acgtgtgcga ggtacctgcc
     1921 gacacccgag gggtgggttt cctctcacgc tctcagaaca ggccccgcag cgaggcaaag
     1981 cgcaccgcct gggcgcgggt gcgcacctgc agcttgcggt agatgttctt gatgtgcgtg
     2041 ttcaccgtca gggcgctgat ctgcagctgg gtgccgatct cggcactggt gtagccgctg
     2101 gcaaccatgc gcagcacctc tttttcgcgc tgcgacaggc gctctggctc gtgctctgca
     2161 gtggcctgca caggtgcctg cgcttcgggt gctgcggctg ctggcgccgc gggcgcgcca
     2221 tggtcaaagc gctgcagcag ccggcgcgcc agattgggcg tgatggacgc gccgccattg
     2281 gccacctgca gcaccgcctg ggggtagttg ccgaaccacg aattcttgac gagatagccg
     2341 gtggcgccaa gctcaaaggc atgcaacacc tggtcgtcgt tttccatgat ggagatgacg
     2401 atggcttcgg cggacggccg attggatttc aggtagtcga tgagctcgaa gccctcgcca
     2461 tcgcccaggt tcagatccac cagcagcacg tcgaattcat gctgcttgat ggccttgcgc
     2521 ccttcgcgca cgctgccggc ctgggccacc agcatggtgc gctcatcggc catgagctcc
     2581 tgggcaatga cgcgccgcat gtgcggatcg tcatcgacga gcagcacgcg caccggcttt
     2641 tcgacctggc cggtcatgaa gtccggccac atggacggtg ggctttggtg tacggcaaac
     2701 tggcccgcgc ctggctggct cgcctcggca gaagaggtcg tttgcgcggc gttggcttca
     2761 ttcgaattca gcatgctgtg ctccaggcag tggttcttgc gggcaaacac caacggaaat
     2821 tttggtatcc aaaagctcag acagagcttg gtgaaataac tggaaagtta caccgaaata
     2881 cgctgattta cggcataaaa ctttttcaag ttaccagatt aactttctga aactattgta
     2941 gtgcgaaagt cggaagaaat ctcagtggaa atctcgactg ccgcccagcg cctgcgccaa
     3001 tcgccccagc agcgggctga gatccttgaa gcgctgcccg atcaggtggc gtactgccat
     3061 gccggcatcc ggcgcatccg agcattgcca cacgccctcc acggccagca gcctggcgcg
     3121 catgagcgcg tcgcgccagc gctcgagctg cgcgggccag acgatgacgt tgaccgggcc
     3181 ggtctcgtcc tccagcgtga cgaacatcgt gccctgggcc gtgccgggcc gctggcgcac
     3241 ggtgacgatg ccgcaggcac gcacacgccg cccccggggt atgtcgcgca gttgcaacgc
     3301 cgtggcgatg cgccagcgcg ccaggtgcgc acgcagcagc gccagcgggt ggcggcgcag
     3361 ggtcaggccg gtggctgcgt agtcgaaaag aatgtcctcg ccctcgggcg cggccggcag
     3421 ctgcagggct tcttcgtgca cgggcgcctg ctgtagcaat ggcaatgcat gtagctgtgc
     3481 ggcagcatcc cacatctgct ggcggcggtg gcccgacagc gcgcgtagtg catcggccgc
     3541 ggccagggcc tgcagatcct gccggtcgag ccgcgcgcgc agggccaggt cttcggtgct
     3601 ggcaaagggg ccgtcgtggc gcgcatgcac cagccgccgg gccgcgtcct ggcgcaggcc
     3661 ggccaccagc cgcaagccca ggcgtacggc gggctggggc agttgcacgc catgcacggg
     3721 tgccagcggt tcttccagcg tgcagtccca gtcgctcgcc gtcacggcta tgggcagcac
     3781 acgcacaccg tggcggcgcg cgtcctgcac cagctgagac ggagcgtaaa agcccatggg
     3841 ctgggagttg agcagcgccg ccagaaagca ggccggctcg tggcgcttga gccagctgct
     3901 ggcgtaggcc agcagggcga agctgtaggc gtggctttcg gggaagccgt attcgccaaa
     3961 gcccaggatc tgctggaaca ggcggtcggc aaactcctgc ggatagccgc gtagcagcag
     4021 gccgcgcttg aagcgctcct cgaactggtg cacgccgccc acgcgtttcc atgcggccat
     4081 ggagcggcgc agcgcatcgg cctcgcccgg cgtgaagccc gcgccggcga tggcgacctg
     4141 catcacctgc tcctggaaga tgggcacgcc cagcgtgcgc gccagcgcct cggccagctg
     4201 tgggtattcc agcgggaagg gctcgccagg ccccagccag cgctggcgct cacgcgcccg
     4261 caggtagggg tggaccatgc cgccctggat cgggccgggc cgcacgatgg ccacctgcac
     4321 caccaggtcg tagaagctgc gcggctgcag gcgcggcagc atgctcatct gcgcgcggct
     4381 ctcaatctgg aacacgccca cggtgtcggc gcggcagacc atgtcgtagg tggcggggtc
     4441 ttcctgcgga atatcggcca ggccccaggg ctcgccgcgc agcagggcac gggcatccag
     4501 gcagcggcgc agggcgctga gcatgcccag cgccagcacg tccaccttca agaggcccat
     4561 ggcgtccagg tcgtccttgt cccactggat gatggagcgc cccggcatgg ccgcgggctc
     4621 caccggcacc aggcgcgtga gcttgccctg ggtcagcacg aagccgccca catgctggct
     4681 caggtggcgc ggaaagccgc gcagccggcg cgtgagctcc agccacagcg cggcctggtg
     4741 ggcgggcaag tcttcgccca ggctggcggc cagggcctgc aaatgatcca cggccagcac
     4801 gtcgtcgaac cagtggtgat ccttggcaaa ggcatccacc agggccggcg ccaggcccag
     4861 ggccttgccc acatcgcgca gggcgctgcg cgtgcggtag ctgatgacca cggccgtgag
     4921 ggcggcgcgc tcgcgcccgt atttggcgta gatgtactgg atgaccacct cgcgccgctc
     4981 gtgctcgaag tccacgtcga tgtcgggcgg ctcgctgcgc tccttgctga tgaagcgctc
     5041 gaacagcagg tgtgcgtgct gcggatcgac ggccgtgatg cccaggcagt agcagaccac
     5101 cgaattggcc gccgaccccc tgccctggca aaggatgccc tggctgcgcg cgtaacgcac
     5161 aatgtcgtgc acggtcagga agtacatctc gtagccgcag tcggcaatca agtccagctc
     5221 cttgccgatc tgcgcctgca cccgctcggg cacgccggcg gggtagcggg cgtgcgcgcc
     5281 ctcccaggtc agccaggcca gggtctgctg cgccgtcatg ccgggcagca cggtctccag
     5341 cgggtagcgg tagctgattt catccaggct gaaacggcag cgcgccgcca cggtcagcgt
     5401 ggccgccagc agttcgggcg ggtacaggcc ggcgagttgg ctgcgcgggc gcaggcgctt
     5461 ctcggcatgg ggctgcagcg caaaaccgca atcggccagg ctgcggccca ggcccacggc
     5521 ggtgatcacg tcctgcagcg gcttggccgc gcgcgcatgc aggtgcacgt cgcccgcggc
     5581 caccagcggc aggcccaggc gcgcgccggc ctgcgtgagc gtgcccagcc acaggccatc
     5641 gtccagcgcc aggtgcagtt ccacggccag ccagcatttg taacaaaata ggcctctagc
     5701 gctttccagg caagcgctaa tagctatcaa atcagaagca tctgtaaaaa cctcgcggcg
     5761 cggcgccagc agcagttcgc agccgcgcag cagcgcccag ggcgactgcg catccacccg
     5821 gtagccgcct ttggcggcac acgcgcgcgc ggcgctgatg aattcgcaca ggtcgcccca
     5881 gccctgcagg tcacgcgcca gggccacgat gcgcagctcg ccccacagga attcgctgcc
     5941 cacgatgagc ggcaggccca gctgccgggc cgccacatgg gcgcgcacca cgccggccac
     6001 cgagcattcg tcggtcagcg ccagggcgct gtagcccagg gcgtgggcgc gttcaaccag
     6061 ctcctgcggg tgcgatgcgc cacgctggaa gctgaagttg ctcagcgcgt gcagctcggc
     6121 gtagtcgggt agcatgatcc tag
//
LOCUS       DAOMTQ010000172         6124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3031_length_6124_cov_27.655627, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000172 DAOMTQ010000000
VERSION     DAOMTQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3031_length_6124_cov_27.655627"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PK518_13500"
     CDS             complement(<1..334)
                     /locus_tag="PK518_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HRM49970.1"
                     /translation="MKHARRPLAQLLRVATLSTLLGMSLAHAADDYSDITQLLRAGKA
                     QDALAKADQRISTNPRDPQLRFLRGVAQADIGKQTDAISTFTKLTEDYPELPEPYNNL
                     AVLYANQNQ"
     gene            555..1931
                     /gene="cysS"
                     /locus_tag="PK518_13505"
     CDS             555..1931
                     /gene="cysS"
                     /locus_tag="PK518_13505"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRM49971.1"
                     /translation="MSLRIYNTLTRALEHFSPLEPGHVRMYVCGMTVYDLCHLGHARS
                     MIAFDVVQRWLRASGLAVTYVRNITDIDDKIIRRAVENGETIRSLTDRMIDALHEDAD
                     ALGIERPTHEPRATEYVPQMLGLIQRLEDKGLAYRAGSGDVNYAVRKFPGYGRLSGKS
                     LDELNAGERVAVQDGKLDPLDFVLWKSAKPEEPAEVKWASAYGEGRPGWHIECSAMGC
                     ALLGESFDIHGGGADLAFPHHENEIAQSEGATGKPFAQLWMHNGFINVDNEKMSKSLG
                     NFFTIRDVLKEYDAETVRFFVVRSHYRSPLNYSDLHLDDARAALKRLYTALSLVAPQP
                     VVIDWAQPHAARFKAAMDEDFGTPEAVAVLFELAGEVNRGKSPEVAGLLKALGAHLGL
                     LQDDPEAFLKAGAGLDAAAIEAQIAARAAAKAAKNFAEADRIRGELLAQGIVLKDSAA
                     GTTWEAAQ"
     gene            1977..2624
                     /locus_tag="PK518_13510"
     CDS             1977..2624
                     /locus_tag="PK518_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRM49972.1"
                     /translation="MAAIKKEVIASAPDYWAEACKHLIKKDRVLRRLIPQVGDVALYQ
                     RGDAFTTLARSIVGQQVSVASAQRVWDRLAALPRSMTPRSVLKLKVDDMRAAGLSARK
                     VEYLVDLALHFDSGQLHVKQWDQMDDPAIVAELVAIRGISRWTADMFLIFHLARPNVL
                     PLDDATLIQGISQHYFSGDPVSRSDAREVAQAWNPWCSVASWYIWRSLAPLPVDY"
     gene            2680..3654
                     /locus_tag="PK518_13515"
     CDS             2680..3654
                     /locus_tag="PK518_13515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRM49973.1"
                     /translation="MAKKTFLDFEQPIAELESKIEELRYVQNESAVDISEEIEQLSKK
                     SQQLTKDIYSDLSPWQITKIARHPERPYTMDYVREIFTDFVELHGDRHFADDQSIVGG
                     LARFNGHACMVLGHQKGRDTKERAARNFGMSRPEGYRKALRLMKTAEKFKLPVFTFVD
                     TPGAFPGIDAEERGQSEAIGRNIYEMAQLEVPIITTVIGEGGSGGALAISVADQVIML
                     QYSIYSVISPEGCASILWKTSDKAQDAADAMGITAHRLKALGLVDKIVSEPVGGAHRD
                     HKQMAAFLKRALGDAYRQLSDLKPKELLDRRYERLQSYGRFNDTKAER"
     gene            3721..4728
                     /gene="tilS"
                     /locus_tag="PK518_13520"
     CDS             3721..4728
                     /gene="tilS"
                     /locus_tag="PK518_13520"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRM49974.1"
                     /translation="MTQSFDAAMQAFAPRLPLAVAFSGGADSTALLLACAERWPGQVH
                     AFHVHHGLQVAADDFERHCIALCMRLGLPLSVQRVDARHAPGQSPEDAARQARYKAFE
                     ALAHDRQAQSAIQTIALAQHADDQVETLLLALSRGAGVAGLAAMPAHWQRGGLHWQRP
                     LLGVAGQEVRAWLRARGQAWVEDPTNSDERYTRNRIRRQLLPALQQAFPQFRDTFARS
                     CAHAAQAHELLQELAQADLATVGQPPRIAALRSLSRARQANVLRHWLRSQHGATPTAA
                     QLDALLRQIAACSTRGHRIQLKVGSGHVLREGELLGWYNQPVLDFISARDGGAPKQSS
                     Q"
     gene            4765..6033
                     /locus_tag="PK518_13525"
     CDS             4765..6033
                     /locus_tag="PK518_13525"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRM49975.1"
                     /translation="MALFVHKYGGTSMGSTERIRNVAKRVAKWARAGHQVVVVPSAMS
                     GETNRLLALAKELTPAHANTAYFRELDMLAATGEQASSALLAIALQSEGMPAVSYTGW
                     QVPIRTDSSYTKARIESIDDKRVRADLDAGRVVIITGFQGIDDEGHITTLGRGGSDTS
                     AVAVAAAMKASECLIYTDVDGVYTTDPRVVPQATRLKTVSFEEMLEMASLGSKVLQIR
                     SVEFAGKYKVPMRVLSSFTPWDIDINEEAASGTLITFEEDEQMEQAVVSGIAFNRDET
                     KISVLGVPDKPGIAYQILGPVADANIEVDVIIQNISKDGRTDFSFTVNHNDHTRTLEL
                     LREKVVPQLGALEVVGDTAICKVSIVGIGMRSHVGVAAKMFRVLSEEGINIQMISTSE
                     IKTSVVIEDKYLELAVRALHKAFDLDQSAA"
BASE COUNT         1109 a         1974 c         2021 g         1020 t
ORIGIN      
        1 gctggttctg gttggcgtac agcacggcca ggttgttgta gggctcgggc agctccgggt
       61 agtcctcggt gagcttggta aaggtgctga tcgcgtcggt ctgcttgccg atgtccgcct
      121 gggccacgcc gcgcagaaag cgcagctggg ggtcacgcgg attggtagag atgcgctggt
      181 cggccttggc caacgcgtcc tgcgccttgc cggcgcgcag cagctgggtg atgtcggagt
      241 aatcgtctgc cgcatgggcc agtgacatac ccaacagcgt agacagggtc gcgacgcgca
      301 ggagttgggc aaggggacgg cgtgcgtgct tcattggtag tgtttgtgca agggaaatgc
      361 aggccctgcg ggcggcttat actgcgactg attgtagccg agggggtgtc atgacggcgc
      421 cacgcagccg tccgcccagt cgtgctctgc accaggcagc gagcctcccc gtggccctat
      481 ctggctggcc tttgctggcg tcgtggtagg ccggccggcg gttgcgattc ccacctgttt
      541 ctgaccttga tcccatgagt ttgcgcatct acaacacgct gacgcgtgcc ttggagcatt
      601 tctctccgct cgaacccggc catgtgcgca tgtatgtctg cggcatgacg gtgtacgacc
      661 tgtgccacct gggtcatgcg cgctcgatga tcgcctttga cgtggtgcag cgctggctgc
      721 gcgcctcggg cctggccgtg acctatgtgc gcaacatcac cgacatcgac gacaagatca
      781 tccgccgcgc ggtggaaaac ggcgagacca tacgcagcct gacggaccgc atgatcgacg
      841 ccctgcacga ggacgccgac gctctgggca tagagcgccc cacgcacgag ccgcgcgcca
      901 ccgaatacgt gccgcagatg ctgggcctga tccagcgcct tgaggacaag ggcctggcct
      961 accgtgccgg cagcggcgat gtgaactacg cggtgcgcaa gttcccgggc tacggccggc
     1021 tgtcgggcaa gtcgctcgac gagctgaatg ctggcgagcg cgtggcggtg caggacggca
     1081 agcttgatcc gctggatttt gtgctctgga agagcgccaa gcccgaggag ccggccgagg
     1141 tgaaatgggc cagcgcctac ggcgagggcc ggcccggctg gcatatcgag tgctcggcca
     1201 tgggctgcgc gctgctgggc gagagctttg acatccacgg cggcggtgcc gaccttgctt
     1261 ttccgcacca cgagaacgag atcgcgcaga gcgagggcgc cacgggcaag ccgtttgcgc
     1321 agctgtggat gcacaacggc ttcatcaacg tggacaacga gaagatgtcc aagagcctgg
     1381 gcaacttctt caccatccgc gacgtgctca aggagtacga cgccgaaacc gtgcgcttct
     1441 tcgtcgtgcg cagccactac cgcagcccgc tgaactacag cgacctgcat ctggacgacg
     1501 cgcgcgccgc gctcaagcgc ctgtacacgg cgttgtcgct ggtggcgccc cagccggtcg
     1561 taatcgactg ggcccagccg catgccgcgc gtttcaaggc ggccatggac gaggacttcg
     1621 gcacgcccga ggccgtggcc gtgctgttcg agctggccgg cgaggtcaac cgcggcaagt
     1681 cgcccgaggt tgccggcctg ctgaaggcgc tcggcgccca tctgggcctg ctgcaggacg
     1741 accctgaggc ctttctgaag gccggcgcgg gcctggatgc cgccgccatc gaggcgcaga
     1801 tcgccgcgcg cgcggccgcc aaggcggcga agaacttcgc cgaggccgat cgcatcaggg
     1861 gcgagctgct ggcccagggc atcgtgctca aggactcggc cgcgggcacg acctgggagg
     1921 ccgcgcagta aatgaattca gagtcaaatt ggctgctgac gcagatagga taagcggtgg
     1981 cagctataaa aaaagaagta attgcaagcg cgccggacta ttgggccgag gcctgcaagc
     2041 acctgatcaa gaaggatcgc gtgctgcgcc gcctaattcc gcaggtgggc gacgtggcgc
     2101 tgtaccagcg cggcgacgcc ttcaccacgc tggcgcgttc catcgtcggc cagcaggtga
     2161 gcgtggcgtc ggcgcagcgc gtgtgggaca ggctggcggc gctgccgcgc agcatgacgc
     2221 cgcgcagcgt gctcaagctc aaggtggacg acatgcgcgc ggccggcctg tcggcgcgca
     2281 aggtggagta cctggtcgat ctggccctgc atttcgacag cggccagttg catgtcaagc
     2341 agtgggacca gatggacgac ccggccatcg tcgccgagct ggtggccata cgcggcatca
     2401 gccgctggac ggccgacatg tttctgatct tccacctggc gcggccgaat gtgctgccgc
     2461 tggacgacgc cacgctgatt cagggcatca gccagcatta cttctcgggc gaccccgtga
     2521 gccgcagcga tgcgcgcgag gtggcccagg cctggaatcc ctggtgcagt gtggcgagtt
     2581 ggtatatttg gcgctcgctt gcccccctgc cggttgacta ttgatcacac attgagagca
     2641 gggcggcagg tttcgggcac cgccccgagg agaaacaagt tggctaaaaa aacctttctg
     2701 gatttcgagc aacccattgc cgagctggaa tccaaaatcg aagaactgcg ctatgtgcaa
     2761 aacgaaagcg cggtggatat ttccgaagag atagagcagc tcagcaagaa gagccagcag
     2821 ctcaccaagg acatctacag cgatctgagc ccctggcaga tcacgaagat tgcgcgccat
     2881 cccgagcgcc cctacaccat ggactacgtg cgcgagatct tcacggactt cgtggagctg
     2941 cacggtgacc gccactttgc cgatgaccag tccatcgtcg gcgggctggc gcgcttcaac
     3001 ggccatgcct gcatggtgct gggccaccag aaggggcgcg acaccaagga gcgcgccgcg
     3061 cgcaacttcg gcatgagccg ccccgagggc taccgcaagg cattgcgcct catgaagacg
     3121 gccgagaagt tcaagctgcc ggtgttcacc ttcgtggaca cgccgggcgc cttccccggc
     3181 atcgatgccg aggagcgcgg ccagtccgag gccattggcc gcaacatcta cgagatggcg
     3241 cagctggagg tgcccatcat caccacggtg atcggcgagg gcggctccgg cggcgcgctg
     3301 gccatcagcg tcgccgatca ggtcatcatg ctgcagtact cgatctactc cgtgatcagc
     3361 cccgagggct gcgcctccat tctctggaag accagtgaca aggcgcagga cgcggccgac
     3421 gccatgggca tcacggcgca ccgtctcaag gccctgggtc tggtggacaa gatcgtcagc
     3481 gagcccgtgg gcggggcgca tcgcgaccac aagcagatgg cggccttcct gaagcgtgca
     3541 ttgggcgacg cctaccgcca gctgtccgac ctgaagccca aggaactgct ggaccgccgc
     3601 tatgagcggc tgcaaagcta cggccgcttc aacgacacca aggccgagcg ttaatcacgt
     3661 attcggcgcc acggcagacg gccccgcggg gccgttgtgc tttgtgcgag catccgcccc
     3721 atgacccaga gtttcgatgc cgccatgcag gctttcgcgc cccggcttcc gctggctgtg
     3781 gccttcagcg gcggggcgga ctccacggcg ctgctgctgg cctgcgccga gcgctggccg
     3841 ggccaggtgc atgcctttca cgtacaccat ggcctgcagg ttgcggccga cgatttcgag
     3901 cggcactgca tcgccctttg catgcgcctg ggcctgccct tgagcgtgca gcgtgtcgat
     3961 gcccggcacg cgccaggcca aagccccgag gatgcagcgc gccaggcacg ttacaaggct
     4021 tttgaggctc ttgcccatga cagacaagcg caatcagcta ttcaaacaat agcattggcg
     4081 cagcatgccg acgaccaggt cgaaaccctg ctgctggccc tgtcgcgcgg cgcgggtgtg
     4141 gccggcctgg cggccatgcc tgcgcattgg cagcgcggcg gcctgcactg gcagcggccg
     4201 ctgctgggcg tggcggggca ggaggtacgc gcatggctgc gtgcgcgtgg ccaggcctgg
     4261 gtggaagacc cgaccaacag cgacgaacgc tacacgcgca accgcatccg gcgccagctg
     4321 ctgcccgcgc tgcagcaggc gtttccgcag tttcgcgaca ccttcgcgcg cagttgcgcc
     4381 catgcggccc aggcccacga gttgctgcag gaactggcgc aggccgatct ggccacggtg
     4441 ggccagccgc cgcgcatcgc cgcgctgcgc tctttgagcc gtgcacgcca ggccaatgtg
     4501 ctgcgccact ggctgcgcag ccagcatggc gccacgccca cggccgcgca gctcgatgcg
     4561 ctgctgcgcc agatcgccgc ctgcagcacg cgcgggcacc gcattcaatt gaaggtgggc
     4621 agcggccatg tgctgcgtga aggcgagctg ctcggttggt acaatcagcc ggttttggat
     4681 ttcatttccg cacgggacgg cggcgcacca aaacaatctt cgcaatagcg ccgctcctca
     4741 gcgttccgtc attgcagata ttccatggca ctgttcgttc ataaatacgg cggcacctcg
     4801 atgggctcca ccgagcgcat ccgcaacgtc gccaagcgcg tagccaagtg ggcgcgggcc
     4861 ggccaccagg tggtggtggt tcccagcgcc atgagtggcg agaccaaccg cctgctggcc
     4921 ctggccaagg agctcacacc cgcacatgcc aacacggctt acttccgtga gcttgacatg
     4981 ctggccgcca ccggcgagca ggcctcgtcg gccctgctgg cgattgcgct gcagtccgag
     5041 ggcatgccgg ccgtgagcta taccggctgg caggtgccga tccgcaccga cagcagctac
     5101 accaaggcgc gcatcgagtc catcgacgat aagcgcgtgc gcgccgacct ggatgccggc
     5161 agggtggtca tcatcaccgg cttccagggc atcgacgacg agggccacat caccacgctg
     5221 ggccgtggcg gctccgacac ttcggccgtg gccgtggctg cggccatgaa ggccagcgag
     5281 tgcctgatct acaccgacgt cgatggcgtc tacaccaccg acccgcgtgt cgtgccccag
     5341 gccacgcgcc tgaagaccgt gagcttcgag gaaatgctgg aaatggccag cctgggcagc
     5401 aaggtgctgc agatccgctc ggtggaattt gccggcaagt acaaagtgcc catgcgcgtg
     5461 ctctccagct tcacgccctg ggacatcgac atcaacgaag aggccgcctc cggcaccctg
     5521 atcacctttg aggaagacga acaaatggaa caagccgtcg tttccggcat cgctttcaac
     5581 cgcgacgaaa ccaagatttc cgtgcttggc gtgcccgaca agcctggcat tgcctaccag
     5641 atcctgggtc ccgtggccga cgccaacatc gaagtggacg tgatcatcca gaacatcagc
     5701 aaggatggcc gcaccgactt cagcttcacc gtgaatcaca acgaccacac gcgcacgctg
     5761 gagctgctgc gcgagaaagt cgtgccccag ctgggcgcgc tcgaggtggt cggcgacact
     5821 gccatctgca aggtcagcat cgtcggcatc ggcatgcgca gccatgtggg cgtggcggcc
     5881 aagatgttcc gcgtgctcag cgaggagggc atcaacatcc agatgatctc cacctccgag
     5941 atcaagacct ccgtggtgat cgaagacaag tacctggagc tggccgtgcg cgccctgcac
     6001 aaggccttcg acctggatca gtccgctgcc tgagctttcc ctgaaggggc ctcaaactga
     6061 ggcataatag cgggattgga aacgtgaccg agtggccgaa ggtgctcccc tgctaaggga
     6121 gtat
//
LOCUS       DAOMTQ010000173         5934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3195_length_5934_cov_29.101378, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000173 DAOMTQ010000000
VERSION     DAOMTQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3195_length_5934_cov_29.101378"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK518_13530"
     CDS             complement(<1..523)
                     /locus_tag="PK518_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRM49976.1"
                     /translation="MEKKDQWNIGYWIVAGLLLLTLQNYWQAAKTVEPVPYSEFEKAL
                     AEGRVAEVLVSDRTVTGRLKSPDSRDKTTIVATRVEPDLAERLSKYDVPYARVVESTW
                     LRDVLSWILPTVAFFGVWFFLFRRFAEKQGMGGFLSIGKSRAKVFMEKNTGVTFADVA
                     GVDEAKAELVEIVD"
     gene            complement(551..1981)
                     /gene="cls"
                     /locus_tag="PK518_13535"
     CDS             complement(551..1981)
                     /gene="cls"
                     /locus_tag="PK518_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRM49977.1"
                     /translation="MSDPNGLTWAATLVSLAVAVPTAGHAVIYKRDPRSATLWVLLIA
                     LLPLGGSLLYGLFGINRYQRRARRLFPGADPAVRQDLAPTMPQAVSAPFAGLAHLVGR
                     ATGQSLTSGNRIEPLVDGEQAYPAMLAAIESARHSVALASYIFDSQGIGAQFVDALRR
                     AHERGVQVRVLIDDVYARWTPRSAYRALQRAGVPAATFNPTLIPARLHAAHLRNHRKL
                     LVIDGETGFTGGMNIFSPYWRPDAPGQACHDLHFRLRGPVVAHLMRCFTDDWCDTTGE
                     RLSKGFWGEPPATADEQGTSWARGIEAGPDEALDRMRWTFMGALSAAKHSVRIWTPYF
                     VPDQPMIAALSTAALRGVRIDVLTPANGDHPTVQWAARAHYWQVLEHGVRIFERPGPF
                     DHSKLMLIDGQWCCLGSANWDARSLRLNFEFNVEVYDTALSTRLSSLFDAARDASGEI
                     SAKALRARPLAIRLRDGVARLFTPIL"
     gene            complement(1998..>2326)
                     /locus_tag="PK518_13540"
     CDS             complement(1998..>2326)
                     /locus_tag="PK518_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpC"
                     /protein_id="HRM49978.1"
                     /translation="TLTFDQTLIDHFAEEGYKPEFGARELKRLIRSELETALAREMLG
                     GGIGKADHASARWDDKAERVVFERQEPAAQPAEPEKPDAANVAEAPPNGESKPARKKK
                     SAGGGS"
     assembly_gap    2327..2426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2427..4315)
                     /locus_tag="PK518_13545"
     CDS             complement(<2427..4315)
                     /locus_tag="PK518_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRM49979.1"
                     /translation="MARKQCQVCGQPATVRVEANLNGRHSTMLLCDDHYRQLVRQQKR
                     TVSPLEALFGSRSGLFEDFLGSDFFRIGDDAPSMAADTDEVVDASFGEPAPAGTGTAR
                     RRGSGLASRISEQSEALLQEAARHAAEFGRAEVDTEHLLLALSDSDVVKTILGQFKIK
                     VDDLKRQIESEAKRGDKPFEGEIGVSPRVKDALSRAFVASNELGHSYVGPEHFLIGLA
                     EEGEGLAANLLRRYGLTPQALRQQVSKVVGKGAEDGRAETPTNTPELDKYSRDLTKMA
                     REGKLDPVIGRAQEIETTIEVLARRKKNNPVLIGEPGVGKTAIVEGLAQRMVAGEVPE
                     TLRDKRLVELNINAMVAGAKYRGEFEERVQKVLKEVTEHQGELILFIDEVHTIVGAGQ
                     GGGEGGLDVANVFKPMMARGELNLIGATTLNEYQKYIEKDAALERRFQPVMVPEPTVA
                     QTMMILRGLRDTFEAHHKVSITEDAIIAAAELSDRYITARFLPDKAIDLLDQAAARVK
                     LSATARPVAVQELESELHQLRREQDYVASRKQYDKAAELGKHIETKEAELKKLVEEWE
                     RERASGSAEVKAEHVAQIVSRLTGIPVNELTVEEREKLLHLEQRLHERLVGQDEAVRA
                     VADAVR"
     gene            complement(4409..4978)
                     /locus_tag="PK518_13550"
     CDS             complement(4409..4978)
                     /locus_tag="PK518_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003847761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRM49980.1"
                     /translation="MDIDFKKLAPWNWFKKEQEEQQSTASLPVQRNDLPVAGGPVSPI
                     LQLHREIDRLFDDAFRGFGFPTLAMPRWPSDWPGMLKPALDIQETDKQYKIALEVPGI
                     EEKDIQITLDNDVLLVRGEKRQEQETKDGGFHRVERSYGSFQRALNLPADANQDTIKA
                     AFKNGVLTITMEKREASTPKQGRSIPING"
     gene            complement(5014..5295)
                     /locus_tag="PK518_13555"
     CDS             complement(5014..5295)
                     /locus_tag="PK518_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM49981.1"
                     /translation="MTARQQAIFTLDELAAYLKVGKRTLYRLASHGEIPAFKVGGTWR
                     FRQSEIDRWINDQTQAGRKKEVIRADEQPKSAKHPVSPGRAVRRRRQTE"
     gene            5561..>5934
                     /locus_tag="PK518_13560"
     CDS             5561..>5934
                     /locus_tag="PK518_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRM49982.1"
                     /translation="MHAVDARVVVAKALAADKFAAWCAELPPGCLVALEACGGAHHWA
                     RKLRGYGLDARLIAGHFVTPYRMAGKGRKNGAADAAAICEAATRPHMRFVPVKPPAQT
                     LLASHQLRKPPDSLTQLIPACLG"
BASE COUNT          971 a         1934 c         1821 g         1108 t
ORIGIN      
        1 aatcgacgat ctcaaccaac tccgccttgg cttcatcgac gccagcgaca tcggcgaagg
       61 tgacgccggt gttcttctcc atgaacacct tggcgcggct cttgccgatg ctcaggaaac
      121 cacccatgcc ctgcttctcg gcaaagcggc ggaacaggaa gaaccagacg ccgaagaagg
      181 ccaccgtcgg cagaatccag gagagcacat cacgcagcca ggtgctctcc accacccgcg
      241 catagggcac gtcgtacttg gacagccgct cggccaggtc gggttcgaca cgggtggcca
      301 cgatggtggt cttgtcccgg ctgtccggcg atttcaggcg tccggtgacc gtgcggtccg
      361 acaccagcac ttcggcgacg cgcccctcgg ccagcgcctt ctcgaattcg ctgtagggca
      421 cgggctcgac ggtcttggcc gcctgccagt agttctgcag cgtcagcagc aacaggccgg
      481 cgacgatcca gtagccaatg ttccattgat ctttcttttc catgggcaat gttcctcgca
      541 agtcgtgtcg ctagagaatg ggggtgaaca gacgggccac cccgtcgcgt aagcggatgg
      601 ccagcgggcg ggcacgcaac gccttcgccg atatctcgcc cgacgcatca cgggcggcat
      661 caaaaaggga tgacagccgc gtagacaacg cggtgtcgta cacctccaca ttgaactcga
      721 agttgaggcg caggctgcgc gcatcccaat tggccgaacc caggcagcac cactggccgt
      781 ctatcagcat cagcttgctg tggtcgaacg ggccaggccg ttcgaagatg cgtacaccgt
      841 gctccagcac ctgccagtag tgggcgcgcg cggcccattg caccgtggga tggtcgccgt
      901 ttgcgggcgt cagcacgtcg atacgcacgc cgcgcaacgc ggccgtgctc agcgccgcga
      961 tcatcggctg atcgggcacg aagtaaggtg tccagatgcg caccgaatgc ttcgccgcgc
     1021 tcaaagcgcc catgaaggtc cagcgcatcc tgtccagggc ctcatcggga ccggcctcga
     1081 tgccgcgcgc ccaagaggtt ccttgctcat cagccgttgc gggcggttcg ccccagaaac
     1141 ccttgctgag ccgctcgccc gtggtatcgc accaatcatc ggtgaagcac cgcatcagat
     1201 gcgcaaccac cggcccgcgc agacgaaagt gcaaatcgtg acaggcctgg cccggcgcat
     1261 cgggccgcca atacgggctg aagatgttca tgccgccggt gaaacccgtc tcgccatcga
     1321 tcaccagcag cttgcggtga ttgcgcagat gcgcggcatg caggcgcgcg ggaatcaacg
     1381 tcgggttgaa cgtcgccgcc ggaacgccgg cgcgctgcaa ggcgcggtag gcgctgcggg
     1441 gcgtccagcg ggcatacacg tcgtcgatca gcacccgcac ttgcacgccg cgctcatgag
     1501 cccggcgcag cgcatcgacg aactgcgccc cgatgccttg gctgtcgaag atgtacgaag
     1561 ccagcgcgac gctgtgccgc gccgactcga tggcagcgag catggccggg taggcctgct
     1621 cgccatcgac gagcggctcg atgcggttgc cgctggtcag cgactggccg gtggcacgtc
     1681 ccaccaggtg ggccaggccg gcaaacggcg ccgatacggc ctggggcatc gtaggcgcga
     1741 ggtcctgccg aacagcagga tctgcccccg ggaacagccg ccgcgcccgc cgctggtaac
     1801 ggttgatgcc gaacagccca tacagcagcg aaccgcccag cggcaacaag gcgatcagca
     1861 gtacccacag cgtggccgat cgagggtcac gcttgtagat gaccgcgtgc cccgctgtgg
     1921 gaacagcgac cgccagcgac acgagggtgg ctgcccatgt cagcccattg gggtcggaca
     1981 cgacagcctc ccatcgctca agatccgccg cccgctgact tcttcttgcg cgcaggcttg
     2041 ctctcgccgt tcggcggtgc ctcggccacg ttcgctgcat cgggcttttc aggctcggcc
     2101 ggctgcgcgg ctggctcctg gcgctcgaag accacccgtt cggccttgtc atcccagcgg
     2161 gcgctggcgt gatcggcctt gccgatgcca ccaccgagca tctcgcgtgc cagcgcggtt
     2221 tccagctcgc tgcggatcag ccgcttgagc tcgcgcgcgc cgaactcggg tttgtagcct
     2281 tcctccgcga agtgatcgat caaggtctga tcgaaggtca gcgtcannnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnncgca cggcatcggc caccgcgcgc accgcttcgt
     2461 cctggcccac caggcgctcg tgcagccgct gctccagatg cagcagcttc tcgcgttctt
     2521 ccaccgtcag ctcgttgacc ggaatgccgg tcaggcgcga gacgatctgc gcgacatgct
     2581 cggctttgac ttcggcgctg cccgaggcgc gctcgcgttc ccattcctcg acaagcttct
     2641 tgagttcggc ctctttggtc tcgatgtgct tgcccagctc ggcggccttg tcgtactgct
     2701 tgcgcgaggc cacatagtcc tgctcacgcc gcagctggtg cagttcggac tccagctctt
     2761 gcaccgccac cgggcgggcc gtggccgaca gcttcacgcg tgcggccgcc tggtcgagca
     2821 ggtcgatggc cttgtcaggc aaaaagcgcg cggtgatgta gcggtccgac aactcggcgg
     2881 cggcgatgat cgcatcctcg gtgatgctga ccttgtggtg cgcctcgaag gtgtcgcgca
     2941 ggccgcgcag gatcatcatg gtctgcgcta ccgtcggctc gggcaccatc accggctgga
     3001 agcgacgctc cagcgcggcg tccttctcga tgtacttctg atactcgttg agcgtggtgg
     3061 cgccgatcag gttcagctcg ccgcgcgcca tcatcggctt gaacacgttg gccacgtcca
     3121 gcccgccttc gccgccaccc tggccggcac cgacgatggt gtgcacctcg tcgatgaaga
     3181 gaatcagctc gccctggtgc tcggtcactt ccttgagcac cttctgcacg cgctcctcga
     3241 actcgccgcg gtacttggcg ccggccacca tggcattgat gttgagttcc accaggcgct
     3301 tgtcgcgcag cgtctcgggc acttcgccgg cgaccatgcg ctgcgccagc ccttcgacga
     3361 tggcggtctt gccgacgccg ggctcgccga tcagcaccgg gttgttcttc ttgcgccggg
     3421 ccagcacttc gatggtcgtc tcgatctcct gcgcgcggcc gatgaccgga tcgagcttgc
     3481 cctcgcgcgc catcttggtg aggtcgcgcg aatacttgtc gagttccggc gtgttggtcg
     3541 gcgtctcggc gcggccatcc tcggcccctt tgccgaccac cttgcttacc tgctggcgca
     3601 gcgcttgcgg cgtgaggccg taacggcgca gcaggttggc cgccaaacct tcgccttcct
     3661 cggcgagccc gatcaggaaa tgctccggcc cgacataaga gtggccgagt tcgttggagg
     3721 ccacgaaagc acggctgagc gcgtccttga cccggggcga cacgccgatc tcgccctcga
     3781 acggcttatc gccgcgcttg gcttcggatt cgatctggcg cttgaggtca tcgaccttga
     3841 tcttgaactg ccccaggatg gtcttgacca cgtcgctgtc ggatagcgcc agcagcaggt
     3901 gttcggtatc gacttcggcg cgcccgaact ctgcagcgtg tcgggcggcc tcctgcaata
     3961 gggcctcgga ctgttcgctg atacggctgg cgagcccact gccgcgacgg cgcgcggtgc
     4021 ccgtaccggc cggggcgggt tcgccgaacg aggcatcgac gacctcgtcg gtatcggccg
     4081 ccatggacgg tgcgtcgtca ccgatgcgga agaagtcgct gccaaggaag tcttcgaaca
     4141 gcccgctgcg cgagccgaac aaggcttcca gcggtgagac ggtgcgcttt tgctggcgca
     4201 ccagttggcg atagtgatcg tcacacaaca gcatggtgct gtggcgacca ttgagattgg
     4261 cttccacccg caccgtggcg ggctggccgc agacttggca ttgttttctg gccatgctga
     4321 tgctcctgta aagattgatc tgacgaggca ccgccgatca cggtgcctca tctctttgtg
     4381 gtttttgaga aaaacgcgct gccccgcgtc agccgttgat cgggatcgag cgcccctgct
     4441 tgggcgtact ggcctcgcgc ttttccatcg tgatcgtgag cacgccgttc ttgaaagcgg
     4501 ccttgatcgt gtcctggttg gcatcggccg gcaggttcag agcgcgctga aagctgccgt
     4561 aggagcgctc cacccggtgg aaaccgccgt ctttcgtttc ctgctcctga cgcttttcac
     4621 cgcgcaccag cagcacgtcg ttgtcgagcg tgatctggat gtctttttcc tcgatgccgg
     4681 gtacttccag ggcgatcttg tactgcttgt cggtctcctg gatgtccagc gccggcttca
     4741 gcatgcccgg ccagtccgac ggccagcgtg gcatggccag cgtcgggaaa ccgaagcctc
     4801 gaaacgcgtc gtcgaacagg cggtcgatct cgcgatgcaa ttgcaggatg ggactgacgg
     4861 gcccacccgc caccggcagg tcattgcgct gcaccggcag cgaggcagtg ctctgttgtt
     4921 cttcctgctc ctttttgaac cagttccagg gagccaattt cttgaaatca atgtccatgt
     4981 cacacctcca gaagaaaaat tgaagactct cgctcactcc gtttgcctgc ggcgacggac
     5041 agcgcgccca ggcgacacgg gatgtttcgc tgacttcggc tgctcatctg cgcgtatcac
     5101 ctccttcttt ctgcctgctt gggtctgatc attgatccat cgatcgattt cactttgacg
     5161 aaaccgccac gtcccgccga ccttgaaggc cggaatctcc ccgtgcgagg cgagccggta
     5221 aagcgtccgc ttgccgactt tcaagtaggc agccaactcg tcgagtgtga agatcgcctg
     5281 ctggcgcgct gtcataccac ccccacaagt tccgtcgtca cgcggcctcg cctgaggaaa
     5341 ttcttgcaag atattgcgca taacatgccg acagtcaaga gtcaacatcc agcgatctgc
     5401 tggccgattg atgtgactca atcgtaagca gccaggaaac aaatgttggc tgccaccgcg
     5461 atggtgctcc ccaagtcatc aatctgcagc catgtgaaag gggaagtcca tgaacgagat
     5521 tgcccgagtc ggggtggatc tggccaagcg ggttatctag gtgcatgcgg tggatgcgcg
     5581 cgtggtggtt gccaaggcgc tggccgctga caagtttgcg gcctggtgcg cggagctgcc
     5641 tcccggctgc ctggtggccc tggaggcctg cggcggtgcg catcactggg cacgcaagct
     5701 gcgcggctat gggctggatg cgcggctgat tgcaggccac ttcgtcacgc cctaccgcat
     5761 ggccggcaag ggacgcaaga acggtgcggc cgatgccgca gcgatctgcg aggcggcaac
     5821 ccgcccgcac atgcgcttcg tgccggtgaa gccgccggcg cagacgctgc tggccagcca
     5881 tcagctacgc aaacctcccg attcactcac gcagcttatt cctgcttgcc tagg
//
LOCUS       DAOMTQ010000174         5760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3366_length_5760_cov_19.281683, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000174 DAOMTQ010000000
VERSION     DAOMTQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_5760_cov_19.281683"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PK518_13565"
     CDS             <1..1065
                     /locus_tag="PK518_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM49983.1"
                     /translation="ALQKTTGIVDLDDSSIADAPRQLVLVDRRKAALMGVAQGAIVSA
                     LHAGLAGENATWLRDGQSKYATPVLLQLPAEQQGSLDALLQLPVRTSAGSTVPIRELV
                     TITDTLREQPIYHKDLLPVNLVVADMAGKVDSPLYGMFDARSAIHQITAPDGGQIEEY
                     FIRQPSDPYRGYSLKWDGEWQITYETFRDMGAAYGVGLILIYLLVVAHFGSYLTPLII
                     MAPIPLTIIGVMPGHALLGAQYTATSMIGMIALAGIIVRNSILLVDFIRLQVQAGMEF
                     KQAIVQSAITRAQPILLTGLAAMLGAFFILDDPIFNGLAISLIFGIAVSTVLTLVVIP
                     ILYFMAYRRRVDVVRGEGGA"
     gene            1110..2456
                     /locus_tag="PK518_13570"
     CDS             1110..2456
                     /locus_tag="PK518_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRM49984.1"
                     /translation="MAGFNAKATDLLQAWQAAEQHERELAVARAAHAAADPLRRQADA
                     LWRPGVGVTAAAGWGHGESEMRGAQFSAPGMGTSSGVAFGTSVTSGAATRVALQASQP
                     LYHPERRAQQQQLNLQADMGELQWQAAQQAAMLRTSERYLALAVAQEQLAVLGGQLTA
                     VDKAATEAQDRFDLGAAPITAVHEASAQRAQLQARRLAAQAELDIQRRALADATGLPD
                     ASLQARLPSSVLPPVQALALWQEQAAAGNLGIRLQQLAADLARAEADKHRASAAPRLD
                     LVAQAEQQRLSGSGDYGSGARNRQTNALLGVQFTVPLYTGGWRSAKEEESLARYNEAQ
                     AQLDATRETVARQVHTAWLGLQAGTEQVRALQQALIAGDARQDATRTGYEVGQRTLLD
                     VLHADNDRAATALALAQARSRLVIHRLQLAQLAGQLDAAALAQANQALAPIQPLKQ"
     gene            2499..3107
                     /locus_tag="PK518_13575"
     CDS             2499..3107
                     /locus_tag="PK518_13575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM49985.1"
                     /translation="MTFWDQAFDTPHFKYGTAPNAFVRAQAHRLRPAARVLVPGDGEG
                     RNGVWLAAQGHAVLSVDASGVGLRKAQQWAAEQGVALQTELADLTSWQPAPGAFDGVV
                     LTFVHLPSALRRDVHQRLVRALRPGGWLILEAFTPEQLRYASGGPKDADMLYTLDDLR
                     TDFAGLHEHLAWAGEVWLDEGPGHQGPAQVVRYLAQALGGRA"
     gene            3104..4240
                     /locus_tag="PK518_13580"
     CDS             3104..4240
                     /locus_tag="PK518_13580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRM49986.1"
                     /translation="MTQAARITILGAGFGALATVRELRRRDAGAQITVVSPRPELHYL
                     PSIIWIPSGLRRREDLIVPLGPFFERMGVRHVAAEVTGLTDDGRSVHTTAGDIDNDAL
                     VIATGGRFLKKLPGIENAITPCEGIAAAELIRDRLAAMQGGTIAIGFAANPQEPQAVR
                     GGPMFEFLFGIDTQLRREGRRERFQLVFFNPSQEPGARLGAKTVQGLLAEMARRGIAT
                     HLGHKMLRFEPGKVVTEGGEIAADLILFMPGMTGNAWFDATTLPRSPGGLLRADAQCR
                     IEGWQHCYVVGDSGSFPGPEWAPKQAHMAELHAKAAAANLLDALAGRPEQAGFVPQLA
                     CIIDSGSHGTLVLRSPQRTLVLPPLRLMHWAKRRFEARYLGQYR"
     gene            4265..4474
                     /locus_tag="PK518_13585"
     CDS             4265..4474
                     /locus_tag="PK518_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRM49987.1"
                     /translation="MTSWRVVRIFAGFFILLSLALGIPGSPIFASQWWLAFTAFVGIN
                     LLQSGLTKWCLLETMLRKLGVQPGC"
     gene            4570..5577
                     /locus_tag="PK518_13590"
     CDS             4570..5577
                     /locus_tag="PK518_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM49988.1"
                     /translation="MPASPTNHWSRRQLLAGGAATATALWLPAARAQANWPSKPLRLV
                     VPFAPGGSSEIVARAVAAEVSKTIGQNMFVDNKPGAAGNIAMQEVAGSSDEHTLILGH
                     IGTLAVNPFIFPKLPYDVNRDFAPITLVSKVPSLYVVNPDLPVKNLKEFIAYVKSKPG
                     QLNYGSAGNGSAGHLAFEYLKMVSDIFMLHVPYRGTGPMLTDLMAGRLQAASVGAPAL
                     LGFIKTGKLRCIATGTGQRLAQLPDVPTVAESGFKGFEMTQWYGLMAPSKWQKPHLAR
                     LEAEAVKAVRGPLVRDKLAQETALAAGNTGTEFSAFIQSEQARWKPVIERAQIRPEGM
                     G"
     gene            complement(5594..>5760)
                     /locus_tag="PK518_13595"
     CDS             complement(5594..>5760)
                     /locus_tag="PK518_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRM49989.1"
                     /translation="AGREQLGEAVQALCFLAGANSIFYGEKLLVTGNPDVAADERLLA
                     KLGLNGRRAP"
BASE COUNT          924 a         2048 c         1904 g          884 t
ORIGIN      
        1 gcgctacaaa aaacgacagg tatcgtcgat ctggatgatt cctcgattgc cgatgcaccg
       61 cgccagctgg tgctggtgga tcggcgcaag gcggcgctga tgggcgtggc gcagggcgcc
      121 atcgtcagcg ccctgcacgc aggcctggcc ggcgagaacg ccacctggct gcgcgatggc
      181 cagagcaaat acgcaacgcc ggtgttgctg caactgcccg ccgagcagca gggcagcctg
      241 gacgcgctct tgcagctgcc ggtgcgcacc agcgcaggca gcaccgtgcc cattcgcgag
      301 ctggtgacga tcaccgacac gctgagggag cagcccatct accacaagga tttgctgccg
      361 gtgaatctgg tcgtggccga tatggcgggc aaggtggaca gcccgctcta cggcatgttt
      421 gacgcgcgtt cggctattca ccagatcacc gcgcccgatg gcggccagat cgaggaatat
      481 ttcatccgcc agcctagcga cccctaccgc ggctatagcc tgaagtggga cggcgagtgg
      541 caaatcacct acgagacctt ccgcgacatg ggcgccgcct acggcgtggg gctgatcctg
      601 atctacctgc tggtcgtcgc gcactttggc tcctacctca cgccgctgat catcatggcg
      661 ccgataccgc tcaccatcat cggcgtcatg cccggccacg cgctgctcgg cgcgcagtac
      721 acggccacca gcatgatcgg catgattgcg ctggccggca tcatcgtgcg caactccatc
      781 ctgctggtgg acttcatcag gctgcaggtg caggcgggca tggagttcaa gcaggccatc
      841 gtgcagtcgg ccatcacgcg cgcccagccc attttgctca ccggcctggc ggccatgctg
      901 ggcgccttct tcatcctcga cgaccccatc ttcaacggcc tggcgatctc actgatcttt
      961 ggcatcgcgg tgtccaccgt gctgacgctg gtggtcatac ccattctgta cttcatggcc
     1021 taccgccggc gcgtggatgt ggtgcgcgga gaaggaggcg catgatgact atgaaatatg
     1081 tagctgccag cgctttattg gtaggcgtca tggccggatt caatgccaaa gccaccgatc
     1141 tgctgcaggc ctggcaggcg gccgagcagc atgagcgcga gctggccgtg gcgcgcgccg
     1201 cgcatgccgc ggccgacccg ctgcgtcgtc aggccgacgc gctgtggcgc cccggcgtgg
     1261 gcgtgacggc ggccgccggc tggggccatg gcgagagcga gatgcgcggc gcgcagttct
     1321 ccgcccccgg catgggcacg agcagcggcg tggcgttcgg cacctcggtc accagcggag
     1381 cggccacgcg cgtcgccctg caggccagcc agccgctcta ccaccccgag cgccgcgccc
     1441 agcagcagca actgaatctg caggccgaca tgggtgagct gcaatggcag gcagcccagc
     1501 aggccgccat gctgcgcacc agcgagcgct atctggcgtt ggccgtggcg caagagcagc
     1561 tcgccgtgct gggcggccag ctcaccgccg tggacaaggc cgccaccgag gcgcaagacc
     1621 gcttcgacct aggcgccgcg ccgatcaccg ccgtgcacga ggccagcgcg cagcgcgccc
     1681 agctgcaggc acggcgcctg gccgcgcagg cggagctgga catccagcgc cgcgcgctgg
     1741 ccgatgccac cggcctgccc gatgccagcc tgcaggcgcg cctgccctcc tcggtcttgc
     1801 ccccggtgca agccctggcg ctgtggcagg agcaggccgc cgccggcaat ctgggcatac
     1861 gcctgcagca gctggccgcc gacctggcac gcgccgaggc ggacaagcac cgcgccagcg
     1921 ccgcgcccag gctcgatctg gtggcccagg ccgagcaaca gcgcctgtcg ggcagcggcg
     1981 actacggcag cggcgcgcgc aaccggcaaa ccaacgcgct gctgggcgtg cagttcaccg
     2041 tgccgctgta caccggcggc tggcgctcgg ccaaggaaga agaaagcctg gcgcgctaca
     2101 acgaagccca ggcgcagctg gacgccacgc gcgaaaccgt cgcgcgccag gtgcataccg
     2161 cctggctggg cctgcaggcg ggcaccgagc aggtgcgcgc gctgcagcag gccctgattg
     2221 ccggcgacgc ccggcaggac gccacgcgca ccggctacga ggtcggccag cgcacgctgc
     2281 tcgacgtgct gcacgccgac aacgaccgcg ccgccaccgc gctggccctg gcccaggcgc
     2341 gcagccggct tgtcatccac cggctgcagc tggcgcagct ggccggtcag ctcgatgccg
     2401 ctgcattggc ccaggcgaat caggccctgg cgccaataca acccctaaag caatagctgc
     2461 atgcgcttat ttgtattgcc ttacacggtg atttgaccat gacattctgg gaccaagcct
     2521 tcgacacgcc gcacttcaaa tacggcactg cgcccaatgc cttcgtgcgc gcgcaggccc
     2581 atcgcctgcg gcctgccgcc cgtgtgctgg tgcccggcga tggcgaaggg cgcaacggcg
     2641 tctggctggc ggcgcaaggc catgcggtgc tgtccgtcga tgcctccggc gtcggcctgc
     2701 gcaaggcgca gcaatgggcc gcagagcaag gcgtggcact gcagaccgag ctggccgacc
     2761 tgaccagctg gcagcccgcg ccgggcgcct ttgacggcgt ggtgctgacc tttgtccacc
     2821 tgccctcggc cctgcgccga gacgtgcacc agcgcctggt gcgggcgctg cgccccgggg
     2881 gctggctgat tctggaggcc ttcacgcccg agcaactacg ctacgccagc ggtgggccca
     2941 aggatgccga catgctctac accctggacg atctgcgcac ggacttcgcc ggcctgcacg
     3001 agcatctggc ctgggctggt gaagtatggc tcgacgaagg ccctggccac cagggcccgg
     3061 cccaggtcgt gcgctacctg gcgcaagccc tcggaggccg cgcatgaccc aggccgcccg
     3121 catcaccatc ctcggcgcag gctttggcgc gctggccacg gtgcgcgagc tgcgccggcg
     3181 cgacgcgggg gcgcagatca ccgtcgtctc cccgcggcct gagctgcact acctgcccag
     3241 catcatctgg atacccagcg gcctgcgccg gcgcgaagac ctgatcgtgc cgctggggcc
     3301 gttctttgag cgcatgggcg tgcgccatgt ggcagccgag gtcacggggc tgacggatga
     3361 cggccgcagc gtgcacacca cggccggcga catcgacaac gacgcgctgg tgattgccac
     3421 cggtgggcgc ttcctcaaga agctgccggg cattgagaac gccatcacgc cctgcgaggg
     3481 cattgccgcg gccgagctga tccgcgaccg cctggccgcc atgcagggcg gcaccatcgc
     3541 catcggcttt gccgccaacc cgcaggagcc gcaggccgtg cgcggcgggc cgatgttcga
     3601 atttttgttc ggcatcgata cccagctacg ccgcgaaggc cggcgcgagc gcttccaact
     3661 ggtgttcttc aaccccagcc aggagcccgg cgcacgcctg ggcgccaaga ccgtgcaggg
     3721 cctgctggcc gagatggcgc gccgcggcat cgccacccac ctgggccaca agatgctgcg
     3781 ctttgagccc ggcaaggtgg ttaccgaggg cggcgagatc gccgccgacc tgatcctgtt
     3841 catgcccggt atgaccggca atgcctggtt cgatgccacc accctgcccc gctcgcccgg
     3901 tggcctgctg cgcgccgacg cgcaatgccg catcgagggc tggcagcact gctatgtggt
     3961 cggtgactcg ggcagctttc ccggccccga atgggcgccc aagcaggcgc acatggccga
     4021 actgcatgcc aaggcggcag cggccaacct gctggacgcc ctggcgggca ggcccgagca
     4081 ggccggcttt gtgccgcagc tcgcctgcat catcgactcg ggcagccacg gcaccctggt
     4141 gctgcgcagc ccacagcgca ccctggtgct gccaccgctg cgcctgatgc actgggccaa
     4201 gcgccgtttc gaggcgcgct atctgggtca gtaccgctga tcaatttttt ctaggagaac
     4261 cctcatgacc tcttggcgcg tcgtccgcat ttttgccggc ttcttcatcc tgctgtcgct
     4321 ggcgctgggc attcccggca gcccgatctt cgccagccaa tggtggctgg ccttcaccgc
     4381 ctttgtcggc atcaacctgc tgcaaagcgg gctgaccaag tggtgtctgc tggagaccat
     4441 gctgcgcaag ctgggtgtgc agccgggctg ctgagcgggc gctctggttc ctgcaaaccg
     4501 ctcaagccgc cgtattcgat gtgctggtac gctggcgccg tcattcatcg cctattggag
     4561 acacacacca tgccagcatc ccccaccaac cattggagcc gccgccagct attggccggc
     4621 ggcgctgcca cggccactgc gctatggctg cctgcggcgc gcgcgcaggc caactggcca
     4681 tccaagccgc tgcgcctggt ggtgccgttc gcgcccggcg gcagctcgga gatcgtggcc
     4741 cgcgccgtgg cggccgaggt gtccaagacc attggccaga acatgttcgt ggacaacaag
     4801 cccggcgcgg ccggcaacat cgccatgcag gaggtggcgg gcagcagtga cgagcacacg
     4861 ctgatcctgg gccatatcgg cacgctggcc gtgaacccct tcatcttccc caagctgccc
     4921 tacgacgtga accgcgactt cgcgccgatc acgctggtgt ccaaggtgcc cagcctgtac
     4981 gtggtgaacc ccgatctgcc ggtgaagaac ctcaaggagt tcatcgccta cgtgaagtcc
     5041 aagcccggcc agctgaacta cggctcggcc ggcaacggca gcgccggcca cctggcattc
     5101 gaatacctga agatggtcag cgacatcttc atgctgcatg tgccctaccg cggcaccggc
     5161 ccgatgctga ccgacctgat ggccggacgg ctgcaggcgg cgtcggtggg cgcacccgcc
     5221 ctgcttggtt tcatcaagac cggcaagctg cgctgcatcg ccaccggcac ggggcagcgc
     5281 ctggcccagc tgcccgacgt gcccaccgtg gccgagtccg gcttcaaggg cttcgagatg
     5341 acccagtggt atggcctgat ggccccttcc aagtggcaaa agccgcactt ggccaggctt
     5401 gaggccgagg ccgtcaaggc cgtgcgcggc cctctggtaa gggacaagct ggcccaggaa
     5461 accgcgctag ccgcgggcaa caccggcacc gagttcagcg ccttcatcca gtccgagcag
     5521 gcccgctgga agccggtgat cgagcgcgcg cagatcagac ccgagggcat gggctgatgg
     5581 ctgccgcagt gcgctacggc gcgcgcctgc cgttcaggcc cagtttggcc agcagccgct
     5641 cgtccgcagc gacatccggg ttgcccgtca ccagcagctt ctcgccgtaa aagatggagt
     5701 tggcgccggc cagaaagcac agcgcctgca ccgcctcgcc cagctgctcg cgcccggccg
//
LOCUS       DAOMTQ010000175         5592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3517_length_5592_cov_16.788514, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000175 DAOMTQ010000000
VERSION     DAOMTQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3517_length_5592_cov_16.788514"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PK518_13600"
     CDS             complement(<1..593)
                     /locus_tag="PK518_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM49990.1"
                     /translation="MKRPILAALVALNVALGLGLAAMWITPQGQLRNLQWVRPAAIQP
                     DFQQMLPALPTQQPVSTSAFLALLERPLFSSTRRPPPPPPPPTPAPPPDLLANAQVLG
                     IYQSGAASGIIVRIEGKARRIRLNESINGWQLQSVQERAAIFTSAGQTRELPLLRAKV
                     GSAAADPMPRVPATANAPLPPPTPAAAPSAPEAATPPP"
     gene            complement(602..1186)
                     /gene="gspM"
                     /locus_tag="PK518_13605"
     CDS             complement(602..1186)
                     /gene="gspM"
                     /locus_tag="PK518_13605"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HRM49991.1"
                     /translation="MSAPTSNARLMQQYLGRQVLVPLATVACLIALAAVGGLYVYDKH
                     QWVQARLTDFVEPRYARLMGLKAGTEALAMASTRASELEALYSHAPDQDSNQIGNDAQ
                     QRVRSLLTAAGMTISSSQVLPSKEESGLERIPLSVRAEGDIVALQGALVGMAEQRPVV
                     LLDAINIQAQGVPERGVQRLSVQFNFLILRRLSS"
     gene            complement(1203..2384)
                     /locus_tag="PK518_13610"
     CDS             complement(1203..2384)
                     /locus_tag="PK518_13610"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRM49992.1"
                     /translation="MSPPTTSLQLFGLDLRLLWQEFRTAWQAAAQSRYLAWLTPAAPV
                     RLLRADGTQALWSGRRFAAEPAKGRAAEFEAIEVSDDLVLRKRLHMPEMPVAAIAQAV
                     QLEAQTASPFAAGNLVWGYDITAATAAGARSVELVMAERAQLGRYVDQQRHRLAPALA
                     REPEVWVIGSGGQPIVLSGWGEQARLRAAVQHRRAACALIASALLIGLAMAVTPSLQL
                     RARSIDAIAAFESLQREVAPVVAQRDQFTRSVERLDAVRSVLAEHIDMLRLMTALTKA
                     LPDSTYLQSVQTQGLKLSIHGLTSDSAALMQTLGAVPGFKEVRAPMAATRASGADAEN
                     FRIEIQLDPAVFALTAGTPAQDAPAQPAAAPAPPAAGASASAEAASAAAPRKSRFTSG
                     G"
     gene            complement(2390..3256)
                     /locus_tag="PK518_13615"
     CDS             complement(2390..3256)
                     /locus_tag="PK518_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRM49993.1"
                     /translation="MMRRRSTPGAGRRGIALVAVLWIVAALSIAVTGIVHTIRSETRT
                     IGASRRMLDLQAVGEAAIMLALQGMTAPPQAGQRVPWRRVDLPYDGRVVTVEVNALNG
                     FVDINRAAPELLAALFAVAGGLKGPAAAALAQATVQARQQPDATGQPLRFEAIEDLMR
                     VPGVDYDLYARLRPLITADALGSGKVNAQAAPQELLAVLADGNLARAAALSNARLQGA
                     PTMDTSTLRTDFIDGGVGTRYRMQAFVPMADGSLGVVLRTVDLNPDQRAGLPWRIFNA
                     ETWMHVPPEKGG"
     gene            complement(3253..3912)
                     /locus_tag="PK518_13620"
     CDS             complement(3253..3912)
                     /locus_tag="PK518_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM49994.1"
                     /translation="MTRPPMGAKGFTLVELLVSMALLSMVMVGMLSVMRSMAQAQDRV
                     EHRLDDSDDLRVATAFIDNVMGHVSARKPQRALQPGESPFQFSARPQELIWVGVMPAR
                     FGMGGRSYFRLALEAGQGGQALVLRFAPWDGAARPPDWNAAQSYVIAPVVTSFAMFYE
                     DAWQPVPQWVGAWQRVDSLPARVRIELATGTGPWPLWIVPMRALPANQPGAARFTMGG
                     E"
     gene            complement(3909..4331)
                     /locus_tag="PK518_13625"
     CDS             complement(3909..4331)
                     /locus_tag="PK518_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM49995.1"
                     /translation="MIRAHRRRRVLRGLSLLELLVALTIMALAVGVLYRVLGASVRNV
                     GMLQGQQGAVLLAQSLLATKDAVAPEGWSEAGDSAGYRWQVRSRPYGALSAQPGTTAL
                     HEVAISIHWFDGGQPRQIELHTLRPQRRPVAPTTGAGS"
     gene            complement(4328..4774)
                     /locus_tag="PK518_13630"
     CDS             complement(4328..4774)
                     /locus_tag="PK518_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRM49996.1"
                     /translation="MRRVRGFTLVELLVVFAILALLMGLAPIAFDRMRDAANYRDTLR
                     GVMTEMRAARQQALVARTETRFVVDLAQRNYRVDGHKAHAIAQPLQVRVTMAGVESTG
                     QEAGIRFFPGGGATGGSVDILRPSGEGARLRVDWLSGRVSLEAVTP"
     gene            complement(4800..5237)
                     /gene="gspG"
                     /locus_tag="PK518_13635"
     CDS             complement(4800..5237)
                     /gene="gspG"
                     /locus_tag="PK518_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRM49997.1"
                     /translation="MARSAPFARQTYSRARGFTLIELLVVLAILTLLAGLVGPKVLNQ
                     LGGAKSKTAAVQIADLDKSLELFKLDVGRYPSTEEGLEALVKKPGSANGWNGPYLKGG
                     LPTDPWGHAYRYTSSGGNIEILSLGADGAPGGEGENADIRNTP"
     gene            complement(5266..>5592)
                     /locus_tag="PK518_13640"
     CDS             complement(5266..>5592)
                     /locus_tag="PK518_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM49998.1"
                     /translation="TVGNTVLRQALAKVAPIVKEGGKVVQAITATGIFEPLAINLIRV
                     GEETGRVGSMMLELSNILNREVETGIKRLLTLVEPVLILVLGVLIAAIIVSILLGILS
                     INDLAV"
BASE COUNT          868 a         1819 c         2036 g          869 t
ORIGIN      
        1 ggcggcggcg tggctgcctc gggcgccgaa ggcgcggctg caggggtggg gggcggcaac
       61 ggcgcgttgg ccgtggccgg cacgcgcggc attgggtcag ccgcggcgga gccgaccttg
      121 gcacgcagca gcggcagttc ccgcgtctgg ccggcactgg tgaatatggc ggcccgctcc
      181 tgcacggatt gcagctgcca gccattgatg ctttcgttca ggcgaatgcg gcgggctttg
      241 ccctcgatgc gcacgatgat gccgctggcc gcgccggact ggtagatgcc cagcacctgt
      301 gcgttggcca gcagatcagg cggcggcgcg ggggtgggcg gcggcggcgg cggcggcggg
      361 cggcgcgtgc tggaaaacag cgggcgttcg agcagggcca ggaaggcgct ggtggacacg
      421 ggctgctgcg tgggcagcgc gggcagcatt tgctggaaat cgggctgtat ggccgcgggg
      481 cgcacccact gcaggttgcg tagctgtccc tgcggggtga tccacatggc ggccaggccc
      541 aggcccaggg cgacgttgag ggccacgagc gcggccagga tggggcgttt catcaccaaa
      601 ctcatgacga caggcgccgc agtatgagga agttgaactg caccgacagg cgctgcacgc
      661 cgcgctcggg cacgccctgg gcctggatgt tgatggcgtc gagcagcacc acggggcgct
      721 gctcggccat gcccaccagg gcgccttgca gggcgacgat gtcgccctcg gcgcgcaccg
      781 acagggggat gcgctccagg ccggattctt ccttggaggg cagcacctgg ctgctggaga
      841 tggtcatgcc ggcggccgtg agcaggctgc gcacgcgctg ctgggcgtcg ttgccgatct
      901 ggttggagtc ctggtcgggg gcgtggctgt acagcgcctc gagttcgctg gcgcgtgtgc
      961 tggccatggc cagcgcctca gtgccggcct tcaggcccat caggcgtgcg tagcgcggtt
     1021 cgacaaagtc ggtcaggcgc gcctgcaccc attggtgttt gtcgtagacg tacaggccgc
     1081 cgacggcggc gagcgcaatg aggcaggcca cggtggccag cggcacgaga acttgcctgc
     1141 ccaggtactg ctgcatgagt ctggcgttgg aggtgggtgc ggacatgcca tgtgccatgg
     1201 gttcagcccc ccgaggtgaa gcggcttttg cgcggtgccg cggcgctggc ggcttcggcc
     1261 gacgcgctgg cgccggccgc gggcggcgct ggagcggctg ccggctgcgc cggcgcgtcc
     1321 tgggccgggg tgccggcagt caaggcgaat acggcggggt cgagctgtat ctcgatacgg
     1381 aaattctcgg catcggcgcc gctggcgcgc gtggcggcca tgggggcgcg cacctccttg
     1441 aagccgggca cggcgcccag ggtttgcatc agggcggcag agtccgaggt gaggccgtgg
     1501 atgctgagct tcagcccctg ggtctgcacg ctttggaggt aggtggagtc gggcagggcc
     1561 ttggtgagcg cggtcatgag ccgcagcatg tcgatatgtt cggcgagtac gctgcgcacg
     1621 gcgtcgagcc gctctaccga acgcgtgaac tggtcgcgct gcgcaacgac cggggctacc
     1681 tctcgctgca ggctttcgaa ggcggctatg gcgtcgatgg agcgcgcacg cagctgcagc
     1741 gatggcgtga cggccatggc gaggcctatc agcagggcgc tggcaatgag tgcgcaggct
     1801 gcgcggcggt gctgcacagc ggcgcgcagc cgggcctgct cgccccagcc gctcaggacg
     1861 atgggctgac cgccgctgcc gatcacccac acttcaggct cgcgcgccag tgcgggcgcc
     1921 aggcggtggc gctgctggtc aacgtagcgt ccgagttggg cacgctcggc catgacgagc
     1981 tccacgctgc gcgcgcctgc ggcagtggcc gccgtgatgt catatcccca caccaggttg
     2041 ccagcggcga agggcgaggc cgtttgtgct tcgagctgca ccgcctgggc gattgcggcg
     2101 accggcattt cgggcatgtg caggcgcttg cgcagcacca ggtcatcgct gacttcgatg
     2161 gcttcgaatt cggcggcccg gccctttgcc ggttcggccg cgaaacgcct gccgctccac
     2221 agcgcctgcg ttccgtcggc acgcaacagg cgcaccggcg cggccggggt cagccaggcc
     2281 aggtaacggc tttgcgcagc cgcctgccag gcggtacgga actcctgcca cagcaggcgc
     2341 agatccaggc caaagagttg aagagaggtg gttggtgggg acatgggatt cacccgcctt
     2401 tttccggcgg tacgtgcatc caggtttcag cgttgaatat gcgccacggt aggccggcgc
     2461 gttggtcggg gttgaggtca acggtgcgca aaaccacgcc aagggagccg tcggccatgg
     2521 gcacgaaggc ctgcatccgg tagcgggtgc cgacgccgcc atcaatgaaa tcggtgcgca
     2581 gcgtgcttgt gtccatcgtc ggcgcgcctt gcaggcgggc attcgacagc gcggcggccc
     2641 gcgccaggtt gccgtcggcc agcacggcca gcagctcctg cggggcggcc tgcgcgttga
     2701 ccttgcccga tcccagcgcg tcggcggtaa tgagcgggcg cagtctagca tagaggtcgt
     2761 aatcgacccc cggcacgcgc atcaggtctt cgatggcctc gaaacgcagc ggctgcccgg
     2821 tggcatcggg ctgctggcgc gcctggacgg tggcctgcgc cagggcggcg gcggccggcc
     2881 ccttgagccc gccggccacg gcaaacagcg cagccagcag ttccggcgcg gcccgattga
     2941 tgtccacaaa accgttgagg gcgttgacct cgacagtgac gacgcgcccg tcgtagggca
     3001 agtccacgcg gcgccacggc accctctgcc cggcctgcgg cggtgcggtc atgccctgca
     3061 gcgcgagcat gatggccgcc tcgcccacgg cctgcaggtc gagcatgcgg cgcgatgcgc
     3121 cgatggtgcg cgtttcggat cgaatggtgt gcactatgcc ggtcaccgcg atgctcaggg
     3181 cggccacgat ccacagcacc gcgaccaggg cgatgccgcg ccggcccgcg ccgggggtgc
     3241 ttctgcgtcg catcattcgc cccccatggt gaagcgggcg gcaccgggct ggttggccgg
     3301 cagcgcccgc atgggcacga tccacaaggg ccacggccct gtgcccgtgg cgagttctat
     3361 gcgcacccgc gccggcaggc tgtccacgcg ctgccatgcg ccgacccact gcggcacggg
     3421 ctgccacgcg tcttcgtaaa acatggcaaa cgatgtgacc acgggtgcaa tcacatagga
     3481 ctgggccgcg ttccagtcgg gcggcctggc tgccccgtcc cacggcgcaa agcgcagcac
     3541 caaggcctgg ccaccctgcc ccgcctccaa cgccagccgg aagtaactgc gcccgcccat
     3601 gccaaagcgc gccggcatca cgcccaccca gatcagttcc tggggacgag ccgaaaattg
     3661 gaagggactt tctcccggtt gcagcgcccg ctgcggcttg cgggcggaga catggcccat
     3721 gacgttgtcg atgaaggcgg ttgccacacg caagtcgtcg gagtcgtcaa gccggtgttc
     3781 cacgcggtct tgggcctggg ccatggagcg catcacggac agcatgccca ccatcaccat
     3841 ggacagcagg gccatgctga cgagcaattc cacgagggtg aaaccctttg cgcccatggg
     3901 tggccgggtc atgatccagc ccccgtggtg ggggcaaccg ggcggcgctg cggtcgcagg
     3961 gtgtgcaact cgatctggcg cggctggccg ccatcaaacc aatggatgct gatggcgacc
     4021 tcgtgcagtg cagtggtgcc cggctgcgcc gacaaggcgc catagggccg gctgcgcacc
     4081 tgccagcggt agccagcaga gtcgcccgcc tcgctccagc cctcgggggc gacggcatcc
     4141 ttggtggcca gcagggactg cgcgagcagc acagcccctt gctggccctg cagcatgccg
     4201 acgttgcgca cgctggcgcc caggacgcga tagagcacgc ccacggccag agccatgatg
     4261 gtcagggcca cgagcagctc aagcagggac aggccgcgca gcacccgccg ccgccgatgg
     4321 gcgcgtatca cggagtcacg gcctccagcg agacgcggcc cgacagccag tccacgcgca
     4381 ggcgcgcccc ttcacccgag gggcgaagga tgtccacgct gccgcccgtg gctccgccgc
     4441 ctgggaaaaa gcgtatgcct gcctcttgcc ccgtggactc caccccagcc atggtgacgc
     4501 gcacctgcaa aggctgcgca atggcgtggg ccttgtgacc atcgacgcga tagttgcgct
     4561 gggcgagatc gacgacgaag cgggtttcgg tgcgcgcaac cagcgcttgc tgccgcgccg
     4621 cacgcatttc ggtcatgacg ccgcgcaatg tgtcgcggta gttggcggcg tcacgcatgc
     4681 gatcgaaggc gatgggcgcc aggcccatga gcagggccag tatggcgaac accaccagca
     4741 actcgaccag cgtgaagccg cgcacgcgcc gcatggatca ccgccgccgt acgcggtcgt
     4801 cagggggtgt tgcggatgtc ggcattctcg ccctcgccgc cgggtgcgcc gtccgccccc
     4861 agggagagga tttcgatgtt gccgccgctg ctggtatagc ggtaggcgtg gccccagggg
     4921 tcggtgggca ggccgccctt gaggtagggg ccgttccagc cgttggcgct gccgggcttc
     4981 ttcaccaggg cttccaggcc ttcctcagtg ctgggataac ggcccacgtc gagcttgaag
     5041 agttccagcg atttgtcgag gtcggcgatc tgcacggccg cggtcttgga cttggcgccg
     5101 cccagctggt tgagcacctt gggaccgacc aggccggcca gcagggtgag gatggccagc
     5161 accaccagca gttcgatcag cgtgaagccg cgcgcgcggg aataggtttg gcgggcaaaa
     5221 ggtgcgctac gggccataaa agaggctttc ttgggcaaac aaaaattata cggcgaggtc
     5281 attgatggac aggatgccaa gcaggatgga gacgatgatg gcggcgatga gcacgcccag
     5341 caccaggatg agcacgggct cgaccagggt cagcaggcgt ttgatgccgg tctcgacctc
     5401 gcggttcagg atgttggaca gctccagcat catcgatccg acgcggccgg tttcttctcc
     5461 gactcggatc aggttgatgg ccaaaggttc aaaaatgccg gtggccgtga tggcctgcac
     5521 caccttgccg ccctccttga cgatgggcgc caccttggcc agggcctgac gcagcacggt
     5581 gttgcccacg gt
//
LOCUS       DAOMTQ010000176         5578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3536_length_5578_cov_24.614521, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000176 DAOMTQ010000000
VERSION     DAOMTQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3536_length_5578_cov_24.614521"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1038)
                     /locus_tag="PK518_13645"
     CDS             complement(199..1038)
                     /locus_tag="PK518_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM49999.1"
                     /translation="MSAASPTTAAPASATPLVEVNGIEVIYNHVILVLKGVSLKVPHH
                     SIVALLGGNGAGKTTTLRAISNLLRGERGEVTKGSITLEGERIENLSPAELVKRGVVQ
                     VMEGRHCFAHLTIEENLLTGAYTRSDKGEIAANLEKVYNYFPRLKTRRTSQAAYTSGG
                     EQQMCAIGRAIMSNPRMVLLDEPSMGLAPQIVDEVFHIVKDLNSKEKVTFLLAEQNTN
                     MALKYSDYGYIMESGRIVMDGPAQELANNEDVKEFYLGMGGGERKSFKDVKSYKRRKR
                     WLA"
     gene            complement(1172..2485)
                     /locus_tag="PK518_13650"
     CDS             complement(1172..2485)
                     /locus_tag="PK518_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM50000.1"
                     /translation="MMFKKVAIAAATVAAGMGALLGAPAAQAQEQFQPLLVYRTGQFA
                     PLGIPWADGKQDYLKLVNARGGINGVKIAFEECETAYDTAKGVECYERLKGRAGGTAS
                     FDTQSTGITFAVSDKAPGDKATIVTAGYGLSQSADGRVFEWNFPLLGTYWTGADSMIQ
                     DILKKEKGSLKGKKIALVYHDSPYGKEPIPLLEKRAAKEGFELLKLPVTAPGVEQKST
                     WLQVRQQRPDYVLLWSAGVMTPTALREAQATGYPREKMYGIWWAGSDHDVKDIGAGAK
                     GYNVVTIHNTAEHDKVHEEVKAQLYDKGQGAAKDAKELGAMAHTRGMVISMLQVEAIR
                     AAQEKYGKGKVMTSEQVRWGLENLNLTQERLNELGFGKILKPVKTSCDNHMGTDWSRI
                     AQWDGGQWKVVSDWYQGDKALIDPLVKEYGEKYAKEKNLKVRSCN"
     gene            complement(2544..3608)
                     /locus_tag="PK518_13655"
     CDS             complement(2544..3608)
                     /locus_tag="PK518_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM50001.1"
                     /translation="MFYRENGQFKTSYAADQQIFAVTQDRIAIWLLLAVAFLVVPFVA
                     SNYVFQAILIPLLIMSLGALGLNILVGYCGQISLGTAAFMAVGAYAAYNLQARFEGMP
                     LLLALIGGGLIAMVFGVVFGLPSLRIRGLYLAVATLAAQFFMDWLTTRADWVTNNSSS
                     GSVSAKALAIAGWQIDTPMEKYLLCLGILCVLGLGAKNLVRSSVGREWMAMRDMDVAA
                     AVIGIRPVYAKLSAFAVSSFIVGIAGALWGFVHLGAWEPAAFSLDKSLQLLFMIIIGG
                     LGSVMGSIFGAAFFILLPLFLNQVPHWLGLPISTATATYLEHMVFGALIVFFLIVEPH
                     GLAKLWSTARQKLRVWPFPH"
     gene            complement(3627..4556)
                     /locus_tag="PK518_13660"
     CDS             complement(3627..4556)
                     /locus_tag="PK518_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM50002.1"
                     /translation="MGFFLETLIGGLMVGTLYALIAIGFVLIFKASGVFNFAQGAMVL
                     FSALAMARFAEWIPGWLGMAPGVVGNLLAIVVTLALMVLVAWVIERFALRHLVNQEPI
                     ALLMATLGIAYLLDGLGPMVFGSAVYSINVGMPKDPVFVMDGLIPGGVMLSLEDLYAA
                     CIAALLVVCLSLFFQKTKTGRALRAVADDHQAAQSIGIPLSRIWVIVWSVAGGVALVV
                     GIIWGSKLGVQYSLSLVALKALPVVILGGLTSLPGAIIGGLIIGVGEKLSEVYIGPMV
                     GGGIETWFAYGLALCFLLVRPQGLFGDKIIDRV"
     gene            complement(4577..5362)
                     /locus_tag="PK518_13665"
     CDS             complement(4577..5362)
                     /locus_tag="PK518_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM50003.1"
                     /translation="MTSKKIGDVILDIKNISLRFGGVKALTDISFNVKEHEIRSIIGP
                     NGAGKSSMLNCINGVYTPQEGSITFRGQTFSHMNSRQVAEMGVARTFQNLALFKGMSV
                     IDNIMTGRNMKIKSNLLLQALRWGPAEREEIAHREKVEHIIDFLEIQAWRKTPVGQLP
                     YGLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDMCRFILDVNDEFGTTIVLIEHD
                     MGVVMDISDRVVVLDYGKKIGDGAPNEVRDNEDVIRAYLGTAH"
     gene            complement(5373..>5578)
                     /locus_tag="PK518_13670"
     CDS             complement(5373..>5578)
                     /locus_tag="PK518_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRM50004.1"
                     /translation="DDELTRTRKVRRNFIADKYGVLIDALYDGKSEQFIETQVKFEDG
                     RKGAVSATLQIIDAKTHPARAAA"
BASE COUNT          929 a         1780 c         1824 g         1045 t
ORIGIN      
        1 acgccgggct gctctgctgg gcgtgggcaa tctggcgcgg cagcgcggcc atgtgggcgg
       61 cctcgcgctc ggccgggctg cgggtttcca gggcgtcgta gaactggctc atgcaatgct
      121 ttcgtctgcg ttcgagggtt tgatgaaaat cagccgccag ggcatatgga ataagcgcta
      181 gcagctatga atattgattt aagccagcca gcgcttgcgc cgcttgtagc tcttgacgtc
      241 cttgaagctc ttcctttcgc cgccgcccat gcccaggtag aactccttca cgtcctcgtt
      301 gttggccagt tcctgggccg ggccgtccat gacgatgcgc ccgctttcca tgatgtagcc
      361 gtagtccgag tatttgagcg ccatgttggt gttctgctcg gccagcagga aggtgacctt
      421 ttccttgctg ttgaggtctt tgacgatgtg gaacacctcg tccacgatct gcggcgccag
      481 gcccatggag ggctcatcga gcagcaccat gcgcgggttg ctcatgatgg cgcggccgat
      541 ggcgcacatc tgctgctcgc cgcccgaggt ataggccgcc tggctggtgc gccgggtttt
      601 caggcgcgga aagtagttgt agaccttctc caggttggcg gcgatttctc ccttgtcgct
      661 gcgcgtgtag gcgccggtga gcaggttctc ctcgatggtc aggtgggcaa agcagtgccg
      721 cccctccatg acctgcacca cgccgcgctt gaccagctcg gcgggtgaga ggttctcgat
      781 gcgctcgccc tccagggtga tgctgccctt ggtgacctcg ccgcgctcgc cgcgcagcag
      841 gttggagatg gcgcgcagcg tggtggtctt gccggcgccg ttgccgccca gcagggccac
      901 gatgctgtgg tgcggcacct tcagggagac gcccttgagc accaggatca catggttgta
      961 gatgacttcg atgccgttga cctccaccag cggcgtggcg ctggcggggg cggcagtggt
     1021 gggggatgcg gcgctcatgg ggtcacctga tgctcattgt ttgatagctg ttaccgctta
     1081 tcagataagc gtttaaatgg agtttgactg ctattgcacg gcggcgcagg ccaggtggat
     1141 gcgccaggcg cacccaccag gaatggacgc atcagttgca gctacgcacc ttgaggttct
     1201 tctccttggc gtacttctcg ccgtattcct tgaccagggg gtcgatcaag gccttgtcgc
     1261 cctggtacca gtcggagacc accttccatt gaccgccgtc ccactgggcg atgcgcgacc
     1321 agtcggtgcc catgtggttg tcgcagctgg tcttgacggg cttgaggatc ttgccgaagc
     1381 ccagctcgtt caggcgctcc tgcgtcaagt tgaggttctc caggccccag cgcacctgct
     1441 cggaggtcat caccttgccc ttgccgtact tttcctgcgc cgcgcggatg gcctcgacct
     1501 gcagcatgga gatcaccatg ccgcgcgtat gggccatggc gccgagttcc ttggcgtcct
     1561 tggccgcgcc ctggcccttg tcgtagagct gggccttgac ctcctcgtgc accttgtcgt
     1621 gctcggcggt gttgtggatg gtgaccacgt tgtagccctt ggcgccggcg ccgatgtcct
     1681 tcacgtcatg gtccgagccg gcccaccaga tgccgtacat cttctcgcgc gggtagcccg
     1741 tggcctgggc ctcgcgcagg gccgtgggcg tcattacgcc ggccgaccac agcagcacat
     1801 agtccgggcg ctgctggcgc acctgcagcc aggtggactt ttgctccacg ccgggcgccg
     1861 tcaccggcag tttcagcagc tcgaagccct ccttggcggc gcgcttttcc agcagcggga
     1921 tgggctcctt gccgtagggc gagtcgtggt agacgagggc gatcttcttg cccttcaggc
     1981 tgcccttctc cttcttcagg atatcctgga tcatggagtc ggccccagtc cagtaggtgc
     2041 ccagcagcgg gaagttccac tcgaacaccc ggccgtcggc cgactgcgac aggccgtagc
     2101 ccgcggtgac gatggtggcc ttgtcgccgg gcgccttgtc gctgacggcg aaggtgatgc
     2161 ccgtggactg ggtgtcaaag ctggcggtgc cgccggcgcg gcccttgagg cgctcgtagc
     2221 actccacgcc cttggcggtg tcgtaagcgg tctcgcattc ctcgaacgcg atcttcacgc
     2281 cgttgatgcc gccccgggcg ttgaccagct tcaggtagtc ctgcttgccg tcggcccatg
     2341 gaatgcccag cggggcgaac tggccggtgc ggtacaccag caggggctgg aactgctcct
     2401 gtgcctgtgc ggcgggtgcg cccagcaggg cacccatgcc ggcggccacg gtggcggcgg
     2461 cgatcgcgac tttcttgaac atcatgtctc tgtctccttc gggttgtgaa aggcagtcct
     2521 ggccgggctg ccagggggtg aaatcaatgc gggaagggcc acacgcgcag cttctggcgc
     2581 gccgtggacc acagcttggc caggccgtgg ggctcgacga tcaggaagaa cacgatcagc
     2641 gcgccgaaca ccatgtgctc caggtaagtg gcggtggcgg tggagatcgg caggcccagc
     2701 caatgcggca cctggttcag gaacagcggc agcaggatga agaaggccgc gccaaagatg
     2761 ctgcccatga cggagcccag gccgccgatg atgatcatga acagcagctg cagcgatttg
     2821 tccaggctga aggccgccgg ctcccaggcg cccaggtgca cgaagcccca cagcgccccg
     2881 gcaatgccga cgatgaagct gctcacggca aaggccgaca gcttggcata caccgggcgg
     2941 atgccgatca cggcggcggc cacgtccatg tcgcgcatgg ccatccattc gcggcccacc
     3001 gagctgcgca ccaggttctt ggcgcccagc ccgagcacgc acaggatgcc caggcacagc
     3061 aggtacttct ccatgggcgt atcgatttgc cagccggcaa tggccagggc cttggcgctg
     3121 accgagcccg aggaggaatt gttcgtcacc cagtcggcgc gggtggtgag ccagtccatg
     3181 aagaactgtg ccgccagagt ggccacggcc aggtacaggc cgcggatgcg cagggacggc
     3241 aggccgaaca ccacgccgaa caccatggca atcaggccgc cgccgatcag cgccagcaac
     3301 agcggcatgc cctcgaagcg cgcctgcagg ttgtaggcgg cataggcgcc cacggccatg
     3361 aaggccgccg tgcccagcga gatctgaccg cagtagccga ccaggatgtt caggcccagg
     3421 gcgcccagcg acatgatgag cagcggaatc aggatggcct ggaagacata gttgctggcc
     3481 acgaagggca cgaccagaaa ggccacggcc agcagcagcc agatggcgat gcggtcctgg
     3541 gtgacggcaa agatctgctg gtcggccgcg tagctggtct tgaactggcc gttctcacga
     3601 taaaacatgt tcttgttcct tcaggctcag acgcggtcga tgatcttgtc cccaaacagg
     3661 ccttgggggc gcacgagaag aaagcacagc gccaggccat aggcaaacca ggtttctatg
     3721 ccgccgccca ccatcgggcc gatgtagacc tcggagagct tctcgcccac gccgatgatg
     3781 agcccgccga tgatggcgcc gggcagcgag gtcaggccgc ccaggatcac caccggcagg
     3841 gctttcaggg ccaccagcga cagcgagtac tgcacgccca gcttggagcc ccagatgatg
     3901 cccaccacca gggccacgcc gccggccacc gaccagacga tgacccagat gcgcgacagc
     3961 ggaatgccga tcgactgggc cgcctggtgg tcgtcggcca cggcgcgcag cgcgcgcccg
     4021 gtcttggtct tctggaagaa caggctcagg cagaccacca gcagcgcggc gatgcaggcg
     4081 gcgtacagat cctccaggct gagcatgacg ccgccgggaa tcaggccgtc catgacgaac
     4141 accgggtcct tgggcatgcc cacgttgatg ctgtagacgg cgctgccgaa caccatgggc
     4201 cccaggccgt cgagcaggta ggcaatgccc agcgtggcca tcaagagtgc gatgggctcc
     4261 tggttcacca ggtggcgcag ggcaaagcgc tctatcaccc aggccaccag caccatcagg
     4321 gccagcgtga ccacgatggc cagcaggttg cccaccacgc ccggcgccat gcccagccag
     4381 ccgggaatcc actcggcaaa gcgcgccatg gccagggccg agaacagcac catggcgcct
     4441 tgcgcgaagt tgaagacgcc cgaggccttg aagatcagca cgaagccgat ggcgatcagc
     4501 gcgtacagcg tgcccaccat caggccgccg atcagggttt cgagaaaaaa tcccatgttc
     4561 tgtgctcctg ctgggtttaa tgggccgtgc ccagataggc gcggatcacg tcttcgttgt
     4621 cgcgcacctc gttcggcgcg ccgtcgccga tcttcttgcc gtagtccagc accaccacgc
     4681 ggtcggagat gtccatcacc acgcccatgt cgtgctcgat gagcacgatg gtggtgccga
     4741 actcgtcgtt cacgtccagg atgaagcggc acatgtcctg cttttcctcc acgttcatgc
     4801 cggccatggg ctcgtccagc agcagcacct gcggttccat ggccagggca cgccctaagt
     4861 ccacgcgctt ttgcaggccg tagggcagct ggcccacggg ggtcttgcgc caggcctgga
     4921 tttcgaggaa gtcgatgatg tgctcgacct tctcgcggtg cgcgatctcc tcgcgctcgg
     4981 ccgggcccca gcgcagcgcc tgcagcagca ggttgctttt gatcttcatg ttgcgcccgg
     5041 tcatgatgtt gtcgatcacg ctcatgccct tgaacagcgc caggttctgg aaggtgcgcg
     5101 ccacgcccat ctcggccacc tgacggctgt tcatgtggct gaaggtctgg ccgcggaagg
     5161 tgatggagcc ctcctgcggc gtgtagaccc cgttgatgca gttgagcatg gagctcttgc
     5221 cggcgccgtt ggggccgatg atggagcgga tctcatgctc cttgacgttg aaggaaatgt
     5281 ccgtcaaggc cttcaccccg ccaaagcgca ggctgatgtt cttgatgtcg agaatgacat
     5341 cgccgatctt cttgctggtc atgctgggtc tttcaggcgg cggcgcgggc cgggtgggtc
     5401 ttggcgtcga tgatctggag cgtggcgctg accgcgccct tgcgcccgtc ctcgaacttc
     5461 acctgggttt cgatgaactg ctcgctcttg ccgtcataca gcgcgtcgat gagcacgccg
     5521 tacttgtcgg cgatgaagtt gcgccgcacc ttgcgcgtgc gcgtgagctc gtcgtcgt
//
LOCUS       DAOMTQ010000177         5527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3595_length_5527_cov_25.663194, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000177 DAOMTQ010000000
VERSION     DAOMTQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3595_length_5527_cov_25.663194"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="rpsA"
                     /locus_tag="PK518_13675"
     CDS             complement(<1..1296)
                     /gene="rpsA"
                     /locus_tag="PK518_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRM50005.1"
                     /translation="MRTRKHMSESFAALFEESLQRTEMRPGEVITAEVVRVEHNFVVV
                     NAGLKSEAYVPIDEFKNDQGEIEVQVGDFVSVAIGSIENGYGDTILSRDTAKRLASWL
                     SLEKALESGEFVTGTTSGKVKGGLTVLVNGIRAFLPGSLIDTRPVKDLTPYENKTLEF
                     KVIKLDRKRNNVVLSRRAVVEASMGEERAKLMETLREGAIVNGVVKNITEYGAFVDLG
                     GIDGLLHITDMAWRRVRHPSEVVTAGQEIMAKILKFDTEKNRVSLGLKQMGDDPWMGV
                     SRRYPSATRLFGKVTNIADYGAFVELEPGIEGLVHVSEMDWTNKNVAPNKLVSLGDEV
                     EVMVLEIDEDKRRISLGMKQCRANPWQEFAQNTKRGDRVKGPIKSITDFGVFVGLAAG
                     IDGLVHLSDLSWNETGEAAVRSYKKGQEVEAIVLAVDVDR"
     gene            complement(1441..3474)
                     /locus_tag="PK518_13680"
     CDS             complement(1441..3474)
                     /locus_tag="PK518_13680"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HRM50006.1"
                     /translation="MFAIPFLDLPPLVGAAGSVQLPGSKSISNRVLLLAALSQGTTEV
                     HDLLASDDTRVMLDALRELGCTVDEAGNGLVRITGLGGAAPRSPARLFMGNAGTAMRP
                     LSAALALLGGEYELSGVPRMHERPIGDLVDALRQLGCHIDYLGNDGYPPLRIAHGAGL
                     PPLQLQAPIRVRGDVSSQFLTALLMALPLVAIKQDVVIEVVGELISRPYIHITLELLA
                     RFGIAVRHDNWQRFVIPAGSRYQSPGSIHVEADASSASYFIALGAIAAGANGQNGIKI
                     EGVGLDSIQGDIRFVEAARAMGAQITGGPNWLHITRGSWPLKAISLDCNHIPDAAMTL
                     AAMALYAEGTTELTNIASWRVKETDRLAAMAIELTKLGASVEEGADYLRVTPPATARD
                     WRAASIHTYDDHRVAMCFSLAAFNPARLPVRIEDPKCVAKTFPDYFEALFSLAQADAA
                     QLPVICIDGPTASGKGTVAAAVAERLGYHFLDSGALYRVTALAAVRAGIPISAENEAA
                     IAQLARQLPVRFAGSRIWLGDEDVSEAIRTEEAGMNASRVSALPLVRKALIALQHSFA
                     RLPGLVADGRDMGTVIFPTAPLKVFMTASAACRADRRYKQLISKGISANIFDLRADLE
                     ARDARDQNRSAAPLKPAQDALPLDNSALTIDETVNQVLHWWQERQPFASRAAG"
     gene            complement(3474..4358)
                     /locus_tag="PK518_13685"
     CDS             complement(3474..4358)
                     /locus_tag="PK518_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRM50007.1"
                     /translation="MFEQLGLIGCGLMGGSFALAMKKAGLVQRVVGYSKSPSTTERAR
                     QLGVIDVEAPSALLAAAGADIVLLAVPVAATEATLKAIKHLVTPQMLIMDVGSTKADV
                     VQAAQRALRDQFGSFVPAHPITGREVSGVEHAEADLYRGRQVILTPTERTLTAQLRRA
                     EGLWTQLGSRVSSMSPESHDASFAAVSHLPHMLAFAMLSSIIGQPQADDLLALAGPGF
                     RDFTRIAASDPKMWRDILRANRDEVLAQSQLFKQALQGLEDAMRASSDQPLEDLITLA
                     SHTRAHWRMGAQRGHKDA"
     gene            complement(4368..5465)
                     /gene="pheA"
                     /locus_tag="PK518_13690"
     CDS             complement(4368..5465)
                     /gene="pheA"
                     /locus_tag="PK518_13690"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRM50008.1"
                     /translation="MTEPQASPDLAGLRVQIDSIDQQLLTLLNQRAQVAEQVGEVKKR
                     EGTPFFRPDRVAQVIEKITRANPGPLKGAHVAAIWREIMSACLALESPQRVAVLGPEG
                     TFCEQAAIEYFGGAADLMYCANFDEVFHATAAGSAQYGVVGVENSNEGVVTRSLDMFL
                     HTPCHVVGEVSLLVRHNLLRQVNNAEHIEAVLAHPQALAQCQAWLSKHLPHAERRPVS
                     SNAEGARLAATNPAWAALASERAAQQYGLHVVAHAIQDEAYNRTRFAVICLPHTLATA
                     APSGHDCTSLIISVPNRPGAVHDLLVPLKKYGVSMTRFESRPARTGQWEYYFYIDIEG
                     HPAEPNVAQALGELQQLCAFYKLLGTYPIAA"
BASE COUNT          854 a         1749 c         1955 g          969 t
ORIGIN      
        1 gcggtccacg tccacggcca gcacgatggc ttcgacttcc tggcccttct tgtagctgcg
       61 cacggcggct tcgccggttt cattccagga caggtcggac aggtgcacca ggccgtcgat
      121 gccggcggcc agacccacga acacaccaaa gtcggtgatc gacttgatcg gacccttgac
      181 gcggtcgccg cgcttggtgt tctgggcaaa ttcctgccag gggttggcgc ggcactgctt
      241 catgcccagg gagatgcggc gcttgtcttc gtcgatctcc agcaccatga cctcgacctc
      301 gtcgcccagg ctgaccagct tgttgggggc cacgttcttg ttggtccagt ccatctcgga
      361 gacgtgcacc aggccttcga tgccgggctc gagctcgacg aaggcgccgt agtcggcgat
      421 gttggtgacc ttgccgaaca ggcgcgtggc cgacggatag cggcgcgaga cgcccatcca
      481 ggggtcgtcg cccatttgct tcaggcccag gctgacacgg ttcttctcgg tgtcgaactt
      541 gaggatcttg gccatgattt cctggccggc ggtgaccacc tcggaggggt ggcgcacgcg
      601 gcgccatgcc atgtcggtga tgtgcagcag gccgtcgatg ccgcccagat ccacgaaagc
      661 accgtattcg gtgatgttct tgaccacgcc attgacgatg gcgccttcgc gcagcgtctc
      721 catcagcttg gcgcgctctt cgcccatgga ggcttccacc acggcgcggc ggctcagcac
      781 cacgttgttg cgcttgcggt cgagcttgat gaccttgaat tccagggtct tgttctcgta
      841 gggggtcagg tccttgaccg ggcgcgtgtc gatcagcgag ccgggcagga aggcgcggat
      901 gccgttgacc agaacggtca ggccgccctt gaccttgccg ctggtggtgc cggtgacgaa
      961 ttcgccggat tccagggcct tttccaggct cagccaggag gccaggcgct tggcggtgtc
     1021 gcgcgagagg atggtgtcgc cgtagccgtt ttcgatggag ccgatggcca cggacacgaa
     1081 gtcgcccacc tggacttcga tttcgccctg gtcgttcttg aattcgtcga ttggcacgta
     1141 ggcttcggac ttcaggccag cgttcacgac gacgaagttg tgctcaacgc gcacgacttc
     1201 ggcggtgatg acctcgccag gacgcatttc ggtgcgttgc agcgattctt caaacagggc
     1261 ggcaaaagat tcggacatgt gtttccttgt ccgcaccaca gggtttacgg ctgcaccatt
     1321 gcggccgttt ctaagactat gggcggaggg tggtggctag ggtcgccacg gggtaaaagg
     1381 tcaaaaaacc acaccaccaa tgcgtctgac ggcgcgaaag gggcggtgtg ggccggttgt
     1441 tcagcctgcc gcccgagagg cgaaaggctg gcgctcctgc caccagtgca aaacctggtt
     1501 cacggtctcg tcgatcgtga gcgcggagtt gtccagaggc aaagcgtctt gcgcaggctt
     1561 gagcggtgcg gcgctgcggt tttgatcgcg agcgtcgcgc gcctcaaggt ctgcgcgaag
     1621 gtcgaagata ttagccgaaa tccccttgga aatcaactgt ttgtagcgcc ggtcggcgcg
     1681 gcaggcggcg ctggcggtca tgaagacctt cagcggcgcc gtggggaaga tcacggtgcc
     1741 catgtcgcgc ccgtcggcca ccaggcccgg caggcgggca aagctgtgtt gcagcgcgat
     1801 gagcgccttg cgcaccaggg gcagggcgga gacgcgcgag gcgttcatgc cggcctcttc
     1861 ggtacgaatg gcttcgctca cgtcctcgtc gcccagccag atacggctgc ctgcgaagcg
     1921 caccggcagc tgccgcgcca gctgcgcgat ggcggcttcg ttttcggcgc tgatagggat
     1981 gccggcccgc accgccgcca aggccgtgac gcggtagagc gcgcccgagt cgagaaagtg
     2041 atagcccagc cgctcggcca cggcggccgc caccgtgccc ttgcccgagg ccgtgggccc
     2101 gtcgatgcag attaccggga gctgcgccgc gtcggcctgg gcgagcgaaa acagtgcctc
     2161 gaagtaatca gggaaggtct tggccacgca tttcgggtct tcgatgcgca ccggcaggcg
     2221 cgccgggttg aaggccgcga gcgagaagca catggccacg cggtggtcgt cgtaggtgtg
     2281 gatgctggcg gcgcgccagt cgcgcgccgt ggccggaggc gtgacgcgca ggtagtccgc
     2341 gccttcctcg acgctggcgc cgagcttggt cagctcgatg gccatggccg ccaggcggtc
     2401 ggtttccttg acgcgccagc tggcgatgtt ggtcagctcg gtcgtgccct cggcgtacag
     2461 cgccatcgcg gccagcgtca tcgccgcgtc ggggatatgg ttgcagtcca ggctgatggc
     2521 cttgagcggc cagctgccgc gcgtgatgtg cagccagttc ggcccgccgg tgatctgcgc
     2581 acccatggcg cgcgcggcct cgacgaagcg gatgtcgccc tggatggaat ccaggcccac
     2641 gccttcaatt tttatgccat tttggccatt tgcgcccgct gcaattgcgc ctaaagctat
     2701 gaaataacta gcagatgagg catctgcctc gacatggatg ctgcccggcg actggtagcg
     2761 gctgccggcc gggatgacaa agcgctgcca gttgtcgtgc cgcacggcaa tgccaaagcg
     2821 cgccagcaac tctagcgtga tgtggatgta gggcctggag atcagctcgc ccaccacctc
     2881 gatcaccacg tcctgcttta tggccaccag cggcagtgcc atcagcaggg cggtgaggaa
     2941 ctggctggag acatcgccgc gcacgcgtat cggcgcctgc agctgcagcg gcggcagacc
     3001 ggcgccatgc gctatgcgta gcggcggata gccgtcgttg cccagatagt cgatgtggca
     3061 gccgagctgg cgcagcgcat cgaccagatc gccaatcggg cgctcgtgca tgcgcggcac
     3121 gccagacagc tcgtattcgc cgcccagcag cgccagcgcc gcgctcaaag ggcgcatggc
     3181 cgtgccggcg ttgcccatga acagccgtgc cggcgagcgt ggcgcggcgc cacccaggcc
     3241 ggtgatgcgc accaggccgt tgccggcctc gtccaccgtg cagccgagct cacgcagcgc
     3301 gtccagcatc acgcgcgtgt cgtcgctggc cagcaggtca tgcacttcgg tcgtcccctg
     3361 gctcagggcg gccagcagca gcacccggtt ggagatgctt ttggagccgg gcagctgcac
     3421 gctgcccgcc gcgcccacca ggggcggcag gtcgagaaat gggatggcaa acatcaggcg
     3481 tccttgtggc cgcgctgcgc gcccatgcgc cagtgggcgc gggtgtggct ggccagggtg
     3541 atcaggtctt ccagcggctg gtcgctgctg gcgcgcatgg cgtcctccag tccttgcagc
     3601 gcctgcttga acagctgcga ctgcgccagc acctcgtcgc ggttggcgcg caggatgtcg
     3661 cgccacatct tcgggtcgct ggcggcaatg cgcgtgaagt cgcgaaagcc cgggccggcc
     3721 agggccagca ggtcgtccgc ctggggctgg ccgatgatgc tggacagcat ggcgaaggcc
     3781 agcatatgcg gcaggtggct cacggcggca aagctggcgt cgtgtgactc gggggacatg
     3841 ctgctgacgc ggctgcccag ctgtgtccac agcccttcgg cgcggcgcag ctgggccgtg
     3901 agcgtgcgct cggtgggcgt gaggatgacc tggcggccgc ggtacaggtc ggcctcggca
     3961 tgttccacgc ccgagacctc gcgcccggtg atcgggtgtg ccggcacgaa ggagccgaac
     4021 tggtcacgca gggcgcgctg cgcggcctgc accacgtcgg ccttggtcga gcccacgtcc
     4081 atgatcagca tctgcggcgt gaccaggtgt ttgatggcct tgagcgtggc ctcggtggcg
     4141 gctacgggca cggccagcag cacgatgtcg gcgccggccg cggccagcag agcagagggc
     4201 gcctcgacgt cgatcacgcc gagctggcgc gcgcgctccg tcgtcgaggg tgacttgctg
     4261 tagcccacca cgcgctgcac caggccggcc ttcttcatgg ccagggcgaa ggagccgccc
     4321 atcaggccgc aaccgatcag gccgagttgt tcaaacatgg ccgctcctca tgcagcgatg
     4381 gggtaggtgc ccagcagctt gtagaaggcg caaagctgtt gcagctcgcc cagggcctgg
     4441 gccacgttgg gctcggcggg gtggccctcg atgtcgatgt agaagtagta ctcccactga
     4501 ccggtgcgcg cggggcgcga ctcgaagcgc gtcatcgaca cgccgtactt cttcagcggc
     4561 accagcaggt cgtgcacggc gccagggcgg ttcggcaccg agatgatgag gctggtgcaa
     4621 tcgtggcccg agggcgcagc ggtggccagc gtgtgcggca ggcagatgac ggcaaagcgc
     4681 gtgcggttgt aggcctcgtc ctggatggca tgggccacca catgcaggcc atattgctgc
     4741 gccgcgcgct cgctggccag cgccgcccag gccggattcg tggcggccag gcgcgcgccc
     4801 tcggcgttgc tcgacaccgg gcggcgctcg gcgtgcggca gatgcttgga cagccaggcc
     4861 tggcattgcg ccagcgcctg ggggtgggcc agcacggcct cgatgtgttc ggcgttgttg
     4921 acctggcgca gcaggttgtg gcgcaccaag aggctcacct cgcccaccac atggcagggg
     4981 gtgtgcaaaa acatgtccag cgagcgtgtg accacgccct cgttggagtt ctcgaccccc
     5041 accacgccgt actgggcact gcccgcggcc gtggcgtgga agacttcgtc gaagttggcg
     5101 cagtacatca ggtcggccgc gccgccaaag tattcgatgg ccgcctgctc gcaaaacgtg
     5161 ccttcggggc cgagcacggc aacgcgctgg ggcgactcaa gtgccaggca ggccgacatg
     5221 atctcgcgcc agatggcggc cacatgcgcg cccttcagcg ggccggggtt ggcgcgggtg
     5281 attttctcga tcacctgggc gacgcggtcg ggccgaaaga agggcgtgcc ctcgcgcttc
     5341 ttgacctcgc ccacctgctc ggccacctgg gcgcgctggt tgagcagggt gagcagctgc
     5401 tggtcgatgc tgtcgatctg cacgcgcagg ccggccaggt cgggggaggc ttggggttcg
     5461 gtcatggggc gtggtgcgtt gtgcgttgtg cgtggagcga tatgcgtgga gcgatatgcg
     5521 tggggcg
//
LOCUS       DAOMTQ010000178         5497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3634_length_5497_cov_25.230246, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000178 DAOMTQ010000000
VERSION     DAOMTQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3634_length_5497_cov_25.230246"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PK518_13695"
     CDS             <1..909
                     /locus_tag="PK518_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM50009.1"
                     /translation="GALLALRLPWALRLLGLPLLLPALWWQAPRPPPGQFELLAADVG
                     QGQAVLVRTARHALLYDAGPRYHQDSDAGQRVLVPLLRALGERLDLLLLSHGDSDHTG
                     GAAAVLAQQPQAELLASMEAGHPLQALRRARPCVAGQGWHWDGVRFELLHPPQHLLQP
                     APDAAGGRAPKPNALSCVLRITAADGRAALLVGDIERDQEQALLAAAAPLAADLLLVP
                     HHGSKTSSSDAFIAAVAPRLALVQAGYRNRFGHPAPEVAARYTERGITLVQTPQCGAA
                     RWVSGQPQDMHCERQAGRRYWHHPVP"
     gene            1033..2469
                     /locus_tag="PK518_13700"
     CDS             1033..2469
                     /locus_tag="PK518_13700"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRM50010.1"
                     /translation="MTYPDTRLFIAGQWQDAADGKTIAVHNPATGQQIGRVAHAGIAD
                     LDQALAAAQKGFEVWRDMPAIERAKAMRRAATLVRERADAIAAIMTQEQGKPRAEARV
                     ETLAAADIIEWFADEGMRVYGRIVPSRNLAVRQLVVKDPVGVVAAFTPWNFPINQVVR
                     KLGAALAAGCAVLVKAPEETPASPAELIRAFADAGLPAGVVGLVYGNPAEISNYLIPH
                     PVVRKVTFTGSTPVGKMLAGLAGQHMKRVTMELGGHAPVIVCEDADIELAVRASGGAK
                     FRNAGQVCIAPTRFLVHEDVRADFVAALTRHAQALKVGDGLAEGTTMGPLANPRRVAA
                     MADFLGDAVQAGATVCTGGERIGEVGNFFAPTVLDNVPTSARIFNEEPFGPVASVRGF
                     TRLEDAIAESNRLPYGLAAYAYTGSLKNAHLLSQRVEVGMLWINQPATPSAELPFGGI
                     KDSGYGSEGGPEALEACLNTRAISITNV"
     gene            complement(2694..3593)
                     /locus_tag="PK518_13705"
     CDS             complement(2694..3593)
                     /locus_tag="PK518_13705"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRM50011.1"
                     /translation="MPFTTLISAPQLQSLMHSGQPLMVFDCSFDLGDPALGARQYREA
                     HVRGALYADLDKHLSAQHGAPGAHGSVVTSAADQPASGGRHPLPSRERFATWLSRIGF
                     ANDMQAVVYDRNGANYCGRLWWMLKWAGHENVAVLDGGLQAWQAAGGAMAQGDEPAHF
                     QSNFALGAPLRRLVTVQEVLATLGQPCQTIVDARATPRYRGEVEPLDPVAGHIPGALN
                     RPFAQNIGPDGCFKSAEQLRSEFADLLGQRPAASVVHQCGSGVSAIPNLLAMEVAGLG
                     PTALFAGSWSEWCSDPGRPVERG"
     gene            3748..4134
                     /locus_tag="PK518_13710"
     CDS             3748..4134
                     /locus_tag="PK518_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM50012.1"
                     /translation="MLSHTSVTTMLPVKDMARARFFYEGCLGLEPGGLRPDGKFVYQV
                     GGSTLALFPKPGGTKADHTAISFRVPDIVAAIARLERTGVVFEDYDFPDLKTVNHVCV
                     LGAEKAAWFQDTEGNFLCIHEDLPGP"
     gene            complement(4142..5053)
                     /locus_tag="PK518_13715"
     CDS             complement(4142..5053)
                     /locus_tag="PK518_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM50013.1"
                     /translation="MQALWMVLAAFLFASMGVCIKLASAHFNAAELVFYRGLISMAVL
                     WALARHQGVSLATRYPGMHAWRSLIGVTSLGCWFYVIGQLPLPTAMTFNYMSSLWIAA
                     FLVGGTLLSWRPSAEQPRPPLQIPLVLCVLVGFGGILLMMRPSLEGSQGFAGIVGMVS
                     GMATALAYMQVVALSRLGEPEARTVFYFALGCAVAGGAALPFTGTSPWPGWPGLWLLP
                     LGLLAAGGQLCMTLAYARSPSARGTLVVANLQYTGLIFAAIYGITVFGDELPLIAWVG
                     MLLIVGSGIAATVLRARTGQETPQQER"
     gene            complement(5137..>5497)
                     /locus_tag="PK518_13720"
     CDS             complement(5137..>5497)
                     /locus_tag="PK518_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HRM50014.1"
                     /translation="RGAIWLTYYPHIMVEWYPHVLTVSTLYPMGVDKTMNMVEFYYPE
                     EIAAFEREFVEAQQAAYMETAIEDDEIGERMDAGRRALLARGDNQVGPYQSPMEDGMQ
                     HFHEWYRARLGDAVPRG"
BASE COUNT          844 a         1921 c         1933 g          799 t
ORIGIN      
        1 ggcgcgctgc tggcgctgcg cctgccctgg gccctgcgcc tgctggggct gccgctgctg
       61 ttgccggctc tgtggtggca ggcgccacgc ccgccgccgg ggcagttcga gctgctggcg
      121 gccgatgtgg gccagggcca ggcggtgctg gtgcgcaccg cgcgccatgc gctgctgtac
      181 gacgccggcc cgcgctacca ccaggacagc gacgccggcc agcgcgtgct ggtgccgctg
      241 ctgcgcgcgc tgggcgagcg gctggatctg ctgctgctga gccatggcga cagcgaccac
      301 accggcggcg cggcggccgt gctggcacag caaccccagg cggagctgct ggcatcgatg
      361 gaggccgggc acccgctgca ggcgctgcgc cgggcccggc cctgcgtggc ggggcagggc
      421 tggcattggg atggcgtgcg ctttgagctg ctgcacccgc cgcagcacct gctgcaaccg
      481 gcgcctgacg ccgctggggg gcgtgcgccc aaacccaatg cgctgagctg cgtgctgcgc
      541 atcaccgccg ccgatggccg ggccgccctg ctggtgggcg acatagagcg cgaccaggag
      601 caggccctgc tggccgccgc tgccccgctg gccgccgacc tgctgctggt gccgcaccat
      661 ggcagcaaga cctcgtccag cgatgccttc atcgccgccg tggcgccgcg cctggccctg
      721 gtgcaggccg gctaccgcaa ccgctttggt caccccgccc ccgaggtggc ggcgcgctac
      781 accgagcgcg gcatcaccct ggtgcagaca ccgcaatgcg gcgcggcgcg ctgggtctcg
      841 ggccagccgc aggacatgca ttgcgagcgc caggccgggc ggcgctactg gcaccatccc
      901 gtgccgtgat gtggagcaag aattcccgca cctgcagtcg ggtgctggca cctggggtga
      961 gcgccgggtg gcccacggcc tatagtccgt gggttcatct caagcacaac agcaacacaa
     1021 ggagccaagc gcatgaccta ccccgacacc cgacttttca tcgccggcca atggcaggac
     1081 gccgccgacg gcaagaccat cgccgtgcac aacccggcca cgggccagca gatcggccgc
     1141 gtggcccatg ccggcattgc cgatctggat caggccctgg cggcggcgca aaagggcttc
     1201 gaggtctggc gcgacatgcc ggccatcgag cgcgccaagg ccatgcgccg cgccgccacc
     1261 ctggtgcgcg agcgcgccga cgccattgcc gccatcatga cccaggagca gggcaagccc
     1321 cgggccgagg cgcgcgtgga gaccctggcc gcggccgaca tcatcgagtg gtttgccgac
     1381 gagggcatgc gcgtctatgg ccgcatcgtg ccgtcgcgca acctggccgt gcgccagctg
     1441 gtggtgaaag acccggtggg cgtggtggcc gccttcacgc cctggaactt ccccatcaac
     1501 caggtggtgc gcaagctggg cgcggcgctg gccgcaggct gcgcggtgct ggtcaaggcg
     1561 cccgaggaga cgccggccag cccggccgag ctgatccgcg cctttgccga cgccggcctg
     1621 cccgctggcg tggtgggcct ggtgtatggc aacccggccg agatttccaa ctacctgatc
     1681 ccgcaccccg tggtgcgcaa ggtcacgttc accggctcca cgcccgtggg caagatgctg
     1741 gccggcctgg ccggccagca catgaagcgc gtgaccatgg aactgggcgg ccatgcgccg
     1801 gtcatcgtct gcgaggacgc cgacatcgaa ctggccgtgc gcgcctcggg cggcgccaag
     1861 ttccgcaacg ccggccaggt gtgcatcgcc cccacgcgtt ttctggtgca cgaggacgtg
     1921 cgcgcggact tcgtggccgc gctcacgcgc catgcccagg cgctcaaggt gggtgacggc
     1981 ctggcggagg gcacgaccat gggcccgctg gccaacccgc gccgcgtggc cgccatggcc
     2041 gacttcctgg gcgatgcggt gcaggccggc gccaccgtct gcaccggcgg cgagcgcatc
     2101 ggcgaggtgg gcaacttctt tgcccccacg gtgctggaca acgtgcccac cagcgcgcgc
     2161 atcttcaacg aggagccctt cggccccgtg gcctcggtgc gcggcttcac ccggctggag
     2221 gacgccatcg ccgaatccaa ccgcctgccc tatggcctgg cggcctatgc ctataccggc
     2281 tcgctgaaga acgcgcacct gctgtcgcag cgcgtggagg tcggcatgct gtggatcaac
     2341 cagccggcca cgccctcggc cgagctgccg tttggcggca tcaaggactc gggctacggc
     2401 tccgagggcg ggcccgaggc gctggaggcc tgcctgaaca cgcgcgcgat ctcgatcacc
     2461 aacgtgtgag cgccagcgct gaaaagcgct ggcgggtctt cactcctctc ccgcgtgcgg
     2521 gagagggtag gggtgagggt gcaaaaacag ggcgctgcgc cgcatcaccc cctcacccca
     2581 accctctcct ccaaggggac gggggggctg gggcccccgc taaataagcg ttgtgcgcta
     2641 tcaagaaatg tgtgcaccca caagcccgcg aggggcgagg ggcttatgtt tggctagcca
     2701 cgctccaccg gccggcccgg gtcgctgcac cattcgctcc agctgccggc aaacagcgcc
     2761 gtcggcccca ggccggcgac ctccatggcc agcaggttgg ggatggcgct cacgccgctg
     2821 ccgcactggt gcaccacgct ggcggcgggg cgctggccca gcaggtcggc aaattcgctg
     2881 cgtaactgct cggcgctctt gaagcagcca tccgggccga tgttctgcgc aaacgggcgg
     2941 ttgagcgccc ccgggatatg gccggccacg gggtccagcg gctccacctc gccgcggtag
     3001 cgtggcgtgg cgcgcgcgtc cactatggtc tggcatggct ggcccagggt ggccaggact
     3061 tcctgcaccg tgaccaggcg gcgcagcggc gcaccgagcg caaagttgga ctggaaatgc
     3121 gccggctcat cgccctgcgc catggcgccg cccgccgctt gccaggcctg caggccgccg
     3181 tcgagcacgg ccacgttctc gtgcccggcc catttcagca tccaccacag gcggccacag
     3241 taattggcgc cgttgcggtc atagaccacg gcctgcatgt cgttggcaaa gcctatgcgc
     3301 gacagccagg tggcaaagcg ctcgcggctg ggcagcgggt ggcgcccgcc ggaggcgggc
     3361 tgatccgccg ccgacgtgac cacgctgcca tgcgcgccgg gcgcgccatg ctgggcgctc
     3421 aggtgcttgt ccaggtccgc gtacagggcg ccgcggacat gcgcctcgcg gtactggcgc
     3481 gcgcccagcg cggggtcgcc caggtcgaag ctgcaatcga acaccatcag cggctggccg
     3541 ctgtgcatca ggctttgcag ctggggggcg gagataaggg tggtgaaggg catggtggaa
     3601 ctcgtgcggg gtaacggtca ggccaggatt gtggccttgt tgccggcgct ggcgcagggc
     3661 aggcgatcgg cctaagctgt attttcggcc cggcaggccg acccgcgagc gcgcaccggg
     3721 gcccgtgcgg gcgaaaggac tttccgtatg ctttcccaca ccagcgttac caccatgctg
     3781 ccggtcaagg acatggcgcg cgcacgcttt ttctacgagg gctgcctggg cctggagcct
     3841 ggcggcctgc gccccgacgg caagtttgtc taccaggtgg ggggcagcac gctggcgctg
     3901 ttccccaagc ccggcggcac caaggccgac cacacggcca tcagctttcg ggtgccggac
     3961 atcgtggcgg ccattgcccg gctcgagcgc accggcgtgg tcttcgagga ctatgacttt
     4021 cccgacctga agaccgtgaa ccatgtctgc gtgctgggcg ccgagaaggc cgcctggttc
     4081 caggacaccg aaggcaactt cctctgcatt cacgaggatc tgcccggccc gtgatgggcg
     4141 ctcagcgctc ctgttgcggc gtttcctgcc ccgtgcgcgc gcgcagaacg gtggcggcga
     4201 tgccgctgcc cacgatgagc agcatgccga cccaggcgat gagcggcagc tcgtcgccaa
     4261 acaccgtgat gccgtagatg gcggcgaaga tcagcccggt gtactgcagg ttggccacca
     4321 ccagcgtgcc gcgcgcggac ggcgagcgcg cgtaggccag cgtcatgcac agctggccgc
     4381 cggcggccag cagccccagg ggcagcagcc acagcccggg ccagccgggc cagggcgagg
     4441 tgccggtgaa cggcagcgcg gcgccgccgg ccacggcgca gccgagcgcg aaatagaaca
     4501 ccgtgcgcgc ctcgggctcg cccaggcgcg acagggccac cacctgcata taggccagcg
     4561 ccgtggccat gcccgagacc atgccgacga tgccggcaaa gccctggctg ccctccaggc
     4621 tggggcgcat catcagcagg atgccgccaa aacccaccag cacgcacagc accagcggta
     4681 tctgcagcgg cgggcgcggc tgctcggcgc tggggcgcca ggagagcagc gtgccgccga
     4741 ctaggaaggc ggcaatccac aggctgctca tgtagttgaa ggtcatggcc gtgggcagcg
     4801 gcagctggcc gatgacgtag aaccagcagc ccagcgaggt cacaccgatc aggctgcgcc
     4861 aggcatgcat gccggggtag cgcgtggcca ggctcacgcc ctggtgccgc gccagggccc
     4921 acagcacggc catgctgatc aggccgcggt agaacaccag ctcggcggca ttgaaatgcg
     4981 ccgacgccag cttgatgcag acccccatgc tggcgaacag gaaggccgcc aataccatcc
     5041 acagcgcttg catggaaatt tggttttgaa agaaaaaagc cgccagcgct tatgtatcag
     5101 gcgctggcag ctattatttt tatagttaat gcgtgctcag ccacgcggca ccgcgtcacc
     5161 aagccgcgcg cggtaccact cgtggaaatg ctgcatgccg tcttccatgg ggctctggta
     5221 ggggccgacc tggttgtcgc cgcgcgccag cagggcgcgc cggccggcgt ccatgcgctc
     5281 gccgatctcg tcgtcctcga tggcggtctc catgtaggcg gcctgctgcg cctcgacgaa
     5341 ttcgcgctcg aaggcggcga tctcctcggg gtagtagaac tccaccatgt tcatggtttt
     5401 gtccacgccc atggggtaga gcgtggagac tgtcagcaca tgcgggtacc actcgaccat
     5461 gatgtgcggg tagtaggtca gccagatggc gccgcgc
//
LOCUS       DAOMTQ010000179         5491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3640_length_5491_cov_24.323216, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000179 DAOMTQ010000000
VERSION     DAOMTQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3640_length_5491_cov_24.323216"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..779)
                     /locus_tag="PK518_13725"
     CDS             complement(321..779)
                     /locus_tag="PK518_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRM50015.1"
                     /translation="MLSLAAAVDAQPRSNAFDDPFVQATAAMPGCPVPEGPLYSAQEV
                     RELAHERAQHGGSCYRSGRCRLPNSYLYDAEIVPRVRQFIREDGGFDDTSVWVLGQRR
                     IVTLTGCVRSVEQGRALEQAVGLVDDVIGVVNLLHVPGEGVAPRYPLARP"
     gene            complement(849..2141)
                     /locus_tag="PK518_13730"
     CDS             complement(849..2141)
                     /locus_tag="PK518_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM50016.1"
                     /translation="MASYRISAVAGMTTIMAMTTATPTLRQDARTIGLIGLAHGSSHF
                     FHMLLPPLFPFLIADFGFSYSELGLLLSVFFVVSGTGQAMAGFLVDKVGARPVLFAAL
                     ACFVAAGLVAGTAGGYAGLVVAAVLAGLGNAPFHPVDFTILNKRVSPQRLGHGFSVHG
                     ISGNLGWATAPVFMAGIATASGSWRVACLAGAALALLVLAIMVWNRDALDDRQGSWAH
                     QTAKGAAAAPEHPMAFLKLPAVWLCFSFFFWSTCSLSAIQSFAAPSLQNMYGIPLSLT
                     TMVVTGYMLCGAMGMVIGGFLVGRVERLEKTISVCLLASALLLTLVGLGVLPGMLALA
                     VAALAGMGIGIAGPSRDMLIKRAAPPGATGRVYGTVYSGLDLGFSIAAPIFGAMLDAQ
                     MNSSIFYGSALALVLGVVSAGFVGADVAARSRPAAAAA"
     gene            2156..3046
                     /locus_tag="PK518_13735"
     CDS             2156..3046
                     /locus_tag="PK518_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM50017.1"
                     /translation="MPNQPAPDATPAPPPPRPVRVPSYVEALTPHLFVPSRARPVRAK
                     HRELTADTHVRPHSHPWAQVAISTTGVIRLTVPHGAYIVPPSRALWIPPGVEHAVTMV
                     EDADLRTLYFHQPPGRCGPAVARAQEEPWRQCRVLEVSDLLRALVREMPAEPDDGAPL
                     APEALHRERHLSALILDELRRASSVRLGVELPRDKRLRHLCETVLADPTHHETLADWA
                     RDTGASPRTVARLFRQELGCTFTQWRQQVILARAVTLAAGRRPINQIAAELGYNSPSA
                     FSAMVRRTVGLAPGQFLGSC"
     gene            complement(3175..4215)
                     /locus_tag="PK518_13740"
     CDS             complement(3175..4215)
                     /locus_tag="PK518_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRM50018.1"
                     /translation="MHLKTKPLLAAACAVIALTGCETTNMKMGSAESKTVATGAAAGG
                     TAANASSQLEHCQSPLGTVSLVENQDAGWYTILRNEYKLPPTANLLRLLIQQSNCFIV
                     VERGAAGMGAMARERQLMGSGEMRGGSNFGKGQMVASDYGMSPEIIFSNNDAGGIGGV
                     LGGLIGGGSGRAVAAIGGGLKTREASAMLTLVDNRSGVQVAASEGSASKSDFAGFGGI
                     FGGGAGGGLGGYQNTAQGKVITAAFMDAYNQMVVALRNYKAQSVQGQGLGGGGKLGVD
                     GGVAPSQTKAGAAMSMREAQERLNGLGYNVGTPDGAAGPKTAKALREFQQAQGLPVTG
                     KLDTATAGALSR"
     gene            complement(4332..5087)
                     /locus_tag="PK518_13745"
     CDS             complement(4332..5087)
                     /locus_tag="PK518_13745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM50019.1"
                     /translation="MRLPWPLPALAVWAGAWLVFVGLRAVLPPLPALLLACALGVVGS
                     LLAATWWRRGLIALGFPLSAALTGAVALPAWAWLLPLALLLLVYPINAWRDAPLFPTP
                     PGALRGLQHHAALADGAPVLDAGCGLGDGLKALRAAYPRAQLHGLEWSWPLALLCRLR
                     CPWARVRRGDIWAADWSGYALVYLFQRPESMARVAAKASAEMAPGNWLVSLAFELPGV
                     RPSMRLQSFAGHGVWVYRLPLQPMAAGAPAGEL"
     gene            complement(5100..>5491)
                     /locus_tag="PK518_13750"
     CDS             complement(5100..>5491)
                     /locus_tag="PK518_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HRM50020.1"
                     /translation="NLADPVQTMLMAGDIDMGHARALLALDRATQITAGNQIAAKKLS
                     VREAESLVKKLGAEFNLTPQKPKKDKSRDMKRVEEELSDLLMAEVEVRVKKRVKRGSR
                     VEEMGELAIAFGSLEALNGLIERLRGD"
BASE COUNT          950 a         1981 c         1806 g          754 t
ORIGIN      
        1 cgacgatgcg tgcatcgtcg gtgaccatgg ggctggcggc atggctcggg gtggcaaccg
       61 cccccagcat ggtcaggccc aggcaggcaa ggtaacgcgc cggcatgcgc ggcgcgggaa
      121 tgtaaaagtg catggtcagc agcagagggg ccggatgatc ttgcgcccac ccggcccggc
      181 ggtggcacaa ggcccggcga ttgtaggcaa ggcctgcttc acgccatctg cacccgccgc
      241 gccgccatgc gcatggtcaa cgcccacagc cgcctcgcgc tgcgcgagga gcccatgctg
      301 ctcactgtag acgcgggcgc tcaggggcgc gccagcgggt agcgcggcgc caccccctca
      361 cccggaacat gcaacaggtt gaccacgccg atcacgtcgt ccaccagccc cacggcttgt
      421 tccagcgcgc ggccctgctc caccgagcgc acgcagcccg tcagcgtgac tatgcgccgc
      481 tggcccagca cccagacgct ggtgtcatcg aagcccccgt cctcgcgtat gaactggcgc
      541 acgcgcggga cgatctctgc gtcgtacagg taggagttgg gcaaccggca acgacccgag
      601 cgatagcagc tgccgccatg ctgggcgcgt tcatgagcca gctcgcgcac ctcctgcgcg
      661 ctgtagagcg gtccctcggg cacggggcaa ccgggcatgg cggccgtggc ctggacgaag
      721 gggtcgtcaa aggcattgct gcgcggctgg gcgtccacag ccgccgccag cgacagcaaa
      781 acaaggcatg aaaatcggcc gcatcgccct aacataaagc gcaataagct accatttata
      841 tagtaaaact aagctgcggc cgccgcgggc cgcgaccgcg ccgccacatc cgcccccaca
      901 aagcccgccg acaccacgcc cagcaccagg gccagcgccg agccgtagaa gatgctggaa
      961 ttcatctgcg cgtccagcat ggcgccaaaa atgggcgcgg cgatggaaaa gcccagatcc
     1021 aggcccgagt acacggtgcc gtagacgcgc ccggtggcgc ccggcggggc ggcgcgcttg
     1081 atgagcatgt cgcgcgacgg gccggcgatg cctatgccca tgccggccag cgcggccacg
     1141 gccaacgcca gcatgccggg cagcacgccc aggcccacca gcgtaagcag cagggccgag
     1201 gccagcaggc agacgctgat ggtcttctcc agccgctcca cgcggcccac caggaagccg
     1261 ccgatcacca tgcccatggc gccgcacagc atgtagccgg tgaccaccat ggtggtgagc
     1321 gacaggggaa tgccatacat gttctgcagc gacggggcgg caaagctctg gatggcgctg
     1381 agcgagcagg tgctccagaa gaagaacgag aagcacagcc acacggccgg cagcttcagg
     1441 aaggccatgg ggtgctcggg ggctgcggcg gcgcccttgg cggtctggtg cgcccagctg
     1501 ccctggcggt cgtcgagtgc gtcgcggttc cacaccatga tggccagcac cagcagcgcc
     1561 agcgccgcgc cagccagaca ggccacgcgc caggagccgc tggccgtggc aatgccggcc
     1621 atgaacaccg gcgccgtggc ccagcccagg ttgcccgaga tgccatgcac cgagaagcca
     1681 tggcccaggc gctggggcga cacgcgcttg ttgagaatgg tgaagtccac cgggtgaaac
     1741 ggcgcattgc ccagaccggc cagcaccgcc gcgaccacca ggccggcgta gccgccagcc
     1801 gtcccggcca ccagaccggc ggcgacaaag cacgccaggg cggcgaacag caccgggcgc
     1861 gcgcccacct tgtccaccag aaagccggcc atggcctgac cggtgcccga gaccacgaag
     1921 aacaccgaca gcagcaggcc cagctcggaa tagctgaagc cgaaatccgc tatcaggaac
     1981 ggaaacagcg gcggcagcag catgtgaaag aagtgcgagg agccatgcgc caggccgatc
     2041 aggcctatgg tgcgcgcgtc ctggcgcagg gtgggggtag cggtcgtcat ggccatgatg
     2101 gtagtcatgc cggcgacggc cgatatacga tagctggcca attgctgtcg caaacatgcc
     2161 aaaccaaccc gcaccggacg ccacccctgc cccaccgccc cccagaccgg tgcgcgtgcc
     2221 gtcctatgtg gaggcgctca cgccccacct gttcgtgccc agccgcgcgc ggccggtgcg
     2281 cgccaagcac cgcgagctga cggccgacac ccatgtgcgc ccgcacagcc acccctgggc
     2341 gcaggtggcg atctccacca ccggcgtgat tcgcctgacg gtgccgcatg gcgcctacat
     2401 cgtgccgccg tcgcgcgcgc tgtggattcc gcccggcgtg gagcatgccg tgaccatggt
     2461 cgaggacgcc gacctgcgca cgctgtactt ccaccagccg cccgggcgct gcggcccggc
     2521 cgtggcgcgc gcacaggagg agccctggcg ccagtgccgc gtgctggagg tgtccgacct
     2581 gctgcgcgcc ctggtgcgcg agatgccggc cgagcccgac gacggcgccc cgctggcacc
     2641 cgaggccctg caccgtgagc gccacctgag cgccctgatc ctcgacgagc tacgccgcgc
     2701 cagcagcgtg cgcctgggcg ttgagctgcc gcgcgacaag cgcctgcgcc acctgtgcga
     2761 gaccgtgctg gccgacccca cgcaccacga aaccctggcc gactgggcgc gcgacaccgg
     2821 cgccagcccg cgcaccgtgg cgcggctgtt ccgccaggag ctgggctgca cctttaccca
     2881 atggcgccag caggtcatcc tggcgcgcgc cgtgaccctg gccgcgggcc gccggcccat
     2941 caaccagatt gccgccgaac tgggctacaa cagccccagc gccttcagcg caatggtgcg
     3001 ccgcacggtg gggctggcgc cggggcagtt tctcggctct tgttgatgcc gaaacacacc
     3061 cccaataact attattttta tagcttgttg cgcttgtcaa atgggcgcta cagcccgttt
     3121 caagcaaaaa cgcggccccg ggggccgcgc tcatgccaga gggcaaaaaa acgatcagcg
     3181 cgacagcgcg ccggccgtgg ccgtgtccag cttgcccgtc accggcaggc cctgggcctg
     3241 ctggaattcg cgcagcgcct tggccgtctt cgggccggcg gcgccgtcgg gcgtgccgac
     3301 gttgtagccc aggccgttca ggcgttcctg ggcctcgcgc atggacatgg cggcgccggc
     3361 cttggtctgc gagggtgcca cgccaccgtc aacgcccagc ttgccaccgc cgcccaggcc
     3421 ctggccttgc acgctttgcg ccttgtagtt gcgcagcgcc acgaccatct ggttgtaggc
     3481 gtccatgaac gcggccgtga tcaccttgcc ctgggccgtg ttctggtagc cgcccaggcc
     3541 gccgccggcg ccaccgccga agatgccgcc aaagccggca aagtcgctct tggaggcact
     3601 gccctcggag gccgccacct gcacgcccga gcggttgtcc accagggtca gcatggcgct
     3661 ggcctcgcgc gtcttcaggc caccgccaat ggccgccacg gcgcggccgc tgccgccgcc
     3721 gatcaggccg ccaagcacgc cgccaatgcc gccggcgtcg ttgttgctga agatgatctc
     3781 gggcgacatg ccatagtcag aggccaccat ctggcccttg ccaaaattgc tgccgccgcg
     3841 catttcgccc gagcccatca gctggcgctc gcgcgccatg gcacccatgc ctgccgcgcc
     3901 acgctcgacc acgatgaagc agttggactg ctggatcaag aggcgcagca ggttggccgt
     3961 gggcggcagc ttgtattcgt tacgcaaaat ggtgtaccag cccgcgtcct ggttctcgac
     4021 cagcgacacc gtgcccaatg gcgactggca atgctcaagc tggctgctgg cgttggcggc
     4081 cgtgccaccc gcggccgcgc ccgtggccac ggtcttggac tcggcgctgc ccatcttcat
     4141 gttggtggtt tcgcagcccg tcagcgcgat cacggcgcag gcggccgcca gcaggggctt
     4201 ggttttcagg tgcatggagg aactccttcg gttgttgtgt catggatctg cgaagctggg
     4261 gcctgaaccc gcgcgcaagg gacaaaatat taacgccttg ccttgagttg caacaacatt
     4321 aatcggcccg cctacagctc gccagccggc gcgcctgccg ccattggctg caatggcagg
     4381 cggtacaccc acaccccatg gcccgcaaag gactgcaagc gcatgctggg gcgtacgccg
     4441 ggcagctcga acgccaggct gaccagccag ttgcccggag ccatttcggc gctggccttc
     4501 gcggccacgc gggccatgct ttccgggcgc tggaacagat acaccagggc atagccgctc
     4561 cagtcggcgg cccagatatc gccgcggcgc accctggccc aggggcagcg cagacggcac
     4621 aacagggcca gcggccagct ccattccagc ccatgcagct gcgcgcgcgg gtaggccgcg
     4681 cgcagggcct tcaagccatc gcccaggcca cagccggcgt ccagcacagg ggcgccatcg
     4741 gccagcgcgg catgatgctg cagcccgcgc aaggcccccg gcggcgtggg aaacaggggt
     4801 gcgtcacgcc aggcattgat gggatagacc agcagcagca gggccaacgg caacagccag
     4861 gcccaggccg gcagcgccac cgcgcccgtc agcgcggcag agagcggaaa cccgagcgcg
     4921 atcaagcccc gtcgccacca ggtggccgcc agcaggctgc ccaccacccc cagggcgcag
     4981 gccagcagca atgccggcag cggcggcaat accgcgcgca ggccgacaaa caccagccag
     5041 gcaccggccc agacagccaa ggctggcagg ggccaaggca ggcgcatcat gagtgtggct
     5101 cagtcgccgc gcaggcgctc gatcaacccg ttgagcgcct ccagcgagcc gaatgcaatc
     5161 gccagctcgc ccatttcctc gacgcggcta ccccgcttga cgcgcttttt cacgcgcacc
     5221 tcgacctctg ccatgagcag gtcggagagt tcttcttcca cccgcttcat gtcgcgcgac
     5281 ttgtccttct tgggcttttg cggtgtcagg ttgaactcgg cgccgagctt cttgaccagg
     5341 ctttcggcct cgcgcaccga cagcttcttg gccgcgatct ggttgccggc ggtgatctgc
     5401 gtggcgcgat cgagcgccag cagcgcgcgc gcatggccca tgtcgatgtc gccggccatg
     5461 agcatggtct gcaccggatc ggccaggttc a
//
LOCUS       DAOMTQ010000180         5461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3681_length_5461_cov_22.412505, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000180 DAOMTQ010000000
VERSION     DAOMTQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_5461_cov_22.412505"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PK518_13755"
     CDS             <1..1190
                     /locus_tag="PK518_13755"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM50021.1"
                     /translation="PARAAAAQAEAPGLRLLDDPLRLAADPGVDVLLELAGGSAARDW
                     VLAALAQGKHVVTANKALLAEHGEQLAQAAVRHGRVLAYEAAVAGAVPVIKALREGLA
                     GNRIDALAGVLNGTSNYILTRMQAAGLDFATALAEAQALGYAEADPSLDVDGTDAAHK
                     LTLLAANAFGLPPALDAVHVEGLRDLQPGDMAAAAQLGYVVKLLAIARRGAQGMELRV
                     HPALVPAQHALARLDGASNGLLVRADAAGEAFFSGAGAGGEPTASAVLADLVDLARHP
                     GTDGRWGGPTAIPTLGLRADAPAAPLPMAQVRTRQLLRLLPGKTFNEAQVLRQLAHAG
                     VKVERRAWGPAEAAGASSSLLLLTAPVPDGLAAQAAERLQAHTGARVRRLRVEDFGAQ
                     QFE"
     gene            complement(1237..1851)
                     /locus_tag="PK518_13760"
     CDS             complement(1237..1851)
                     /locus_tag="PK518_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRM50022.1"
                     /translation="MKLHTLLSVADFERRAQALLPRAVLGYVNGGTEDGLTLQANRDA
                     LRAVQFRPKGLVGVAERSQRVTLWGRSYAHPFGIAPMGVTAMCRHRCEQDLALAAARH
                     GRVLAAVPRDFPVLIDGGFRRGTDILKAMALGARMVFLGRAMLYGAAVAGQAGVARVI
                     DILGTEIDRNLGLLGCPDVNALDQDFIVRLRAGHPGMQAGYQTL"
     gene            1979..4159
                     /gene="dinG"
                     /locus_tag="PK518_13765"
     CDS             1979..4159
                     /gene="dinG"
                     /locus_tag="PK518_13765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HRM50023.1"
                     /translation="MSAQEWADQALQSFESVVQASAGFRVREGQHLMARQVAETFARA
                     QLGKPEDDVDEPERAIAVIQAGTGVGKSLAYSVPAIAMALARGTRVLISTATVALQEQ
                     LVHKDLPQLAARMEQPFAFALAKGRGRYVCKLKLERLAGGGSGGGDDDLPDDDLFPDE
                     AAQARHPRHDAEARLRFYTSLADALAQGGWDGDRDSLDTPPEPEVWSPVAAEASSCTG
                     RHCPLFNQCSYFERRKQLVAAQVIVANHDLLLSSIGARLLPELDNCLLVIDEAHHLPA
                     TALDQFACEMDLSRLTWLDKLASRALRIGQLVEVEEIADIPNHTARLRAALQDLARLV
                     MDVYGEQLRAPLPGQRSFNSSMPTRARVSGGLLPDALVGPLGQAAHHADGFLQALSAI
                     AKALKSEMRDKPDEARRLSQLYAQIGSLAPRLEGVHACAQLLLQDAPEGAVPAAKWFT
                     LQVEGDFIVVRAHASPVLPGNTLKNHLWSAVRGAVLTSATLTSCGQFDFFLRESGLHG
                     DAAVTTLAVASPFDYARQGLLVATETQADPRDAQAFTAEMVDALLSDLALVEAGALVL
                     FTSREQMRQAVDAMPTAMRPVVLVQNTLPRQQLLARHRERVAQGLPSIIFGMQSFGEG
                     LDLPGSLCESLFITKLPFAPPDDPVGEARAEWLRGAGRDPFSELVVPATAIRLAQWVG
                     RAIRTEDDQAHVYCYDKRLVRTGYGQRLLAGLPPFALQRRQSAA"
     gene            4156..4509
                     /locus_tag="PK518_13770"
     CDS             4156..4509
                     /locus_tag="PK518_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HRM50024.1"
                     /translation="MSQHSIPPCPQCGLTNTYPDGDSYICPDCAFEWPMQAEAAPADA
                     EGSRVRDANGNDLADGDAVILVKDLKVKGSSTVLKKGSKIKGIRLVDGANGHNVDCKT
                     ELGSMLLKSEFLKKA"
     gene            4515..5144
                     /locus_tag="PK518_13775"
     CDS             4515..5144
                     /locus_tag="PK518_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM50025.1"
                     /translation="MAAPIAVIDFETTGMAPAQGARATEVAIVLMEGEQVVDRFDSLM
                     RTGVPIPPFIEQLTGISNAMLATAPPAEAVMREAARFVGSAPMVAHNAAFDSKFWQSE
                     LQHAGLAAPQPFACTVLLSRRVFPDAPSHSLGRITDHLGLPRTGRAHRALADAEMAAA
                     LLARMQRELCQRFGVPWPDHALLMQLQRCARQSIGKLLDRLSTGAAHTS"
BASE COUNT          845 a         1990 c         1868 g          758 t
ORIGIN      
        1 ccccggcgcg ggcggcggcc gcgcaggccg aggcgcccgg cctgcgcctg ctggacgacc
       61 cgctgcggct ggccgccgac cccggcgtgg acgtgctgct ggagctggcc ggcggcagcg
      121 ccgcgcgcga ctgggtgctg gccgcgctgg cccaaggcaa gcatgtggtc acggccaaca
      181 aggccctgct ggccgagcat ggcgagcagc tggcgcaggc cgccgtacgc catggccggg
      241 tgctggccta cgaggccgcc gtggccggcg cggtacccgt catcaaggcg ctgcgcgagg
      301 gcctggcggg caaccgcatc gacgccctgg ccggcgtgct caacggcacc agcaactaca
      361 tcctgacgcg catgcaggcg gcgggcctgg actttgccac cgccctggcc gaggcccagg
      421 ccctgggcta cgccgaggcc gacccgagcc tggacgtgga cgggacggac gccgcacaca
      481 agctgaccct gctggcggcg aacgccttcg gcctgccccc ggcgctggac gccgtgcatg
      541 tcgaaggcct gcgcgacctg caaccgggcg acatggccgc agccgcgcag ctgggctacg
      601 tcgtcaagct gctggcgatc gccaggcgcg gcgcgcaggg catggagctg cgcgtgcacc
      661 cggccctggt gccggcgcag catgccctgg cccggctgga tggggccagc aacggcctgc
      721 tggtgcgcgc cgacgccgcg ggcgaggcct tcttcagtgg cgccggcgca ggcggcgagc
      781 ccacggcctc ggccgtgctc gccgacctgg tggatctggc acgccacccc ggcaccgatg
      841 gccgctgggg cggcccgacc gccataccca ccctgggcct acgcgcggac gccccggccg
      901 cccccctgcc catggcccag gtgcgcacgc gccagctgct gcgcctgctg ccgggcaaga
      961 cgttcaacga ggcccaggtg ctgcgccaac tggcgcacgc cggcgtcaag gtggagcgcc
     1021 gcgcctgggg cccggccgaa gcggcaggcg cgtcgtccag cctgctgctg ctgaccgcgc
     1081 ccgtcccgga cggcctggcc gcgcaggcgg ccgagcgtct gcaggcgcac acgggcgccc
     1141 gggtgcggcg tttgcgcgtg gaggatttcg gcgcccagca gtttgaatga aaatggcctc
     1201 cagcgcccta tgcatagtcg ctaaacgcta taatttttat agtgtttgat atccggcctg
     1261 catgccggga tggccagcac gcaaacgcac gatgaagtcc tgatccagcg cgttcacatc
     1321 ggggcagcca agcaggccca ggttgcggtc gatctccgtg cccaggatgt cgatcacacg
     1381 cgccacgccg gcctggccgg ccacggccgc gccataaagc atggcacgcc ccagaaacac
     1441 catgcgcgcg cccagggcca tggccttgag gatatcggtg ccacggcgaa agcccccgtc
     1501 gatcagcacc ggaaagtcgc gcggcacggc ggccagcacc cggccatggc gtgcggcggc
     1561 cagggccaga tcctgctcac agcgatggcg gcacatggcc gtcacgccca tgggggcgat
     1621 gccaaacggg tgggcataac tgcggcccca cagcgtgacc cgttgcgagc gctcggccac
     1681 gcccaccagc cccttggggc gaaactgcac ggcgcgcagg gcatcgcggt tggcctgcaa
     1741 ggtcaggcca tcctcggtgc cgccattcac gtagccgagc acggcgcgcg gcagcagggc
     1801 ttgtgcgcgg cgttcaaagt cggcaaccga cagcaaggtg tgcagcttca tgacgcgcga
     1861 ttgttgcccg tttcggtgca atgcccctgc ggccgcgggc aggcaacgca atcgactacc
     1921 ataactggac gaaatcacag catccggtgc gccacggttg aagcgcccgc ccccatccat
     1981 gtccgcacaa gaatgggccg atcaggccct gcaatccttt gagtcggtgg ttcaggccag
     2041 cgccggcttt cgcgtgcgcg agggccagca cctgatggcc cgacaggtgg ccgagacctt
     2101 cgcccgggcg cagctgggca agcccgagga cgatgtcgat gaacccgagc gcgccatcgc
     2161 cgtcatccag gccggcaccg gcgtgggcaa gtcgctggcc tacagcgtgc cggcgattgc
     2221 catggcgctg gcgcgcggca cgcgggtatt gatctccacg gccacggtgg cgctgcagga
     2281 gcagctggtg cacaaggatc tgccgcagct tgcggcgcgc atggagcagc cctttgcctt
     2341 tgcgctcgcc aaggggcgcg gacgctatgt ctgcaagctg aagctggagc gcctggccgg
     2401 cggcggcagc ggcggcggcg atgacgatct gcccgacgac gacctgttcc ccgacgaggc
     2461 cgcgcaagca cggcacccgc gccacgacgc cgaggcacgc ctgcgcttct acaccagtct
     2521 ggccgacgcc ctggcccagg gcggctggga cggcgaccgc gacagcctgg acaccccacc
     2581 cgagcccgag gtctggagcc ccgtggcggc cgaggccagc tcctgcaccg gcagacattg
     2641 cccgctgttc aaccagtgca gctacttcga acggcgcaaa cagctggtgg cggcccaggt
     2701 catcgtcgcc aaccatgacc tgctgctgtc ctcgattggc gcgcgcctct tgcccgagct
     2761 ggacaactgc ctgctggtca tcgacgaggc gcaccacctg ccggccacgg cgctcgatca
     2821 gttcgcctgc gagatggatc tgtcgcgcct gacctggctg gacaagctgg ccagccgcgc
     2881 gctgcgcata ggccagctgg tggaggtgga agagatagcc gacatcccca accacaccgc
     2941 acgcctgcgc gccgcgctgc aggacctggc gcggctggtg atggatgtgt atggcgagca
     3001 gctgcgcgcg ccgctgcccg gccagcgctc attcaactca tccatgccca cgcgcgcccg
     3061 cgtgagcggc ggcctgctgc ccgatgcgct cgtcgggccc ctgggtcagg ctgcgcacca
     3121 tgcggacggc tttctgcagg ccctcagcgc catcgccaag gcgctcaaga gcgagatgcg
     3181 cgacaagccc gacgaggcgc gccgcctgtc ccagctgtat gcccagatcg gcagcctggc
     3241 gccacggctg gagggcgtgc acgcttgtgc ccagttgctg ctgcaggacg cgcccgaggg
     3301 cgcggtgccg gcggccaagt ggttcacgct gcaggtcgag ggcgacttca tcgtcgtgcg
     3361 cgcccacgcc agcccggtgc tgcccggcaa cacgctgaag aaccacctgt ggtcggccgt
     3421 gcgcggcgcg gtgctgacat cggccacgct gacgagctgc gggcagttcg acttcttcct
     3481 gcgcgagtcg ggcctgcatg gcgacgccgc cgtgaccacg ctggccgtgg ccagcccctt
     3541 cgactatgcg cgccagggcc tgctggtggc caccgaaaca caggccgacc cacgcgacgc
     3601 ccaggccttc acggccgaga tggtggacgc cctgctgtcc gacctggccc tggtggaggc
     3661 cggcgcgctg gtgctgttca cctcgcgcga gcagatgcgc caggcggtgg acgccatgcc
     3721 gaccgccatg cgccccgtgg ttctggtgca gaacaccctg ccgcgccagc agctgctggc
     3781 gcgccaccgc gagcgcgtgg cccagggcct gccgtccatc atcttcggca tgcagtcctt
     3841 tggcgagggg ctggacctgc cgggcagcct gtgcgaatcg ctgttcatca ccaaactgcc
     3901 ctttgccccg cccgacgacc cggtgggcga ggcgcgcgcc gaatggctgc gcggcgccgg
     3961 gcgcgacccg ttcagcgagc tggtggtgcc ggccaccgcc atccgcctgg cgcaatgggt
     4021 gggccgggcg attcgcaccg aggacgacca ggcccatgtc tactgctacg acaagcgcct
     4081 ggtgcgcacc ggctatggcc agcgcctgct ggccgggctg ccgccgtttg cgctgcagcg
     4141 gcgacaatcg gcggcatgag ccagcacagc attccaccct gcccgcaatg cgggctgacc
     4201 aacacctacc ccgacggcga cagctacatc tgcccggact gcgcctttga atggcccatg
     4261 caggccgagg cggcgccggc cgatgccgaa ggcagccgcg tgcgcgacgc caacggtaac
     4321 gatctggccg acggcgacgc cgtgatcctg gtgaaggatc tgaaggtcaa gggatcgtcc
     4381 acggtgctca aaaagggcag caagatcaag ggcatacgcc tggtggacgg cgccaacggc
     4441 cacaacgtgg actgcaagac cgagctgggc agcatgctgc tcaagtccga gttcctgaaa
     4501 aaggcctgaa ccccgtggcc gcacccatcg ccgtcatcga cttcgagacc accggcatgg
     4561 cgccggccca gggcgcccgt gccaccgagg tggccatcgt gctcatggaa ggcgagcagg
     4621 tcgtcgatcg ctttgacagc ctgatgcgta ccggcgtacc catcccgccg ttcatcgagc
     4681 agctcaccgg catcagcaat gccatgttgg ccacggcgcc gcccgccgag gcggtgatgc
     4741 gcgaggcggc gcgctttgta ggcagcgcgc ccatggtggc gcacaacgct gcgtttgaca
     4801 gcaagttctg gcagtccgaa ctgcagcacg ccggcctggc cgcgccccag ccctttgcct
     4861 gcaccgtact gctgtcgcgc cgcgtgtttc ccgacgcgcc cagccacagc ctgggccgca
     4921 tcaccgacca cctgggcctg ccgcgcaccg gccgcgccca ccgcgcgctg gccgacgccg
     4981 aaatggccgc cgccctgctg gcacgcatgc agcgcgagct gtgccagcgc tttggcgtcc
     5041 cctggcccga tcacgccctg ctgatgcagc tgcagcgctg cgcgcgccag tccatcggca
     5101 agctgctgga caggctgtca acgggcgcgg ctcacacctc atagctattg ttttaatagc
     5161 tatttgcgct taccgtacaa gcgatacagg ccgtttttat ctagttcttg gcgccactca
     5221 aactacttcc ctcactggag gaaggtagcg gccgaaagca tagacagcgc acccgccgcc
     5281 tgcgggacat caggacaccg cccgcagcgc cgccgccaga tcgcgcgcga ggcgccccat
     5341 gtcgcgcagg cccagggccg ccaggttgat gcggatcgcc gggccactgc ccaggcgcag
     5401 cggcgcgccc gcctgcacgg cccagccgcg cgcggccatg tgctgcacca cggcggcctc
     5461 g
//
LOCUS       DAOMTQ010000181         5430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3713_length_5430_cov_27.703256, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000181 DAOMTQ010000000
VERSION     DAOMTQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3713_length_5430_cov_27.703256"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK518_13780"
     CDS             complement(<1..488)
                     /locus_tag="PK518_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRM50026.1"
                     /translation="MSKIKPAPLPPDTVIGGYRVVHKLAAGGFGVVYLAKDAEGQQVA
                     IKEYLPASLATRTAGELLPQVQPEKLSLYRLGLKSFFEEGRSLAQISHASVVSVLNFF
                     RENETVYMVMNHLEGASLQDFIITARELKTEKVFRESTIRSLFDEVLRGLRIVHQHKM
                     LHL"
     gene            572..1465
                     /locus_tag="PK518_13785"
     CDS             572..1465
                     /locus_tag="PK518_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRM50027.1"
                     /translation="MTGYASVQFTGAAAGEGGPGNARRLGLEIRSVNSRFLDLSLRLS
                     DELRAQEPALRALLTQQLKRGKVELRAFVDSAEFAALAEPTPRLLQRVGTVQDTVRSW
                     LPDAAPLSVAEALRLCANAGAPEQDWSGPTMDLAQRALADMVAAREREGERLVAMLLD
                     RLKQLRALAEQAGPLVPRLVEQQRQRFMERWKEAMQLAEGAAVPEAAQDRALTEATAF
                     AIRIDVAEEVTRLQSHLDEIQRLLKKGGEVGKRLDFLIQELHREANTLGSKSAAMELT
                     RISVDMKVLIEQMREQVQNIE"
     gene            1498..2118
                     /gene="gmk"
                     /locus_tag="PK518_13790"
     CDS             1498..2118
                     /gene="gmk"
                     /locus_tag="PK518_13790"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRM50028.1"
                     /translation="MDYPGNLFVVSAPSGAGKSSLVKALMELDARVQPSVSHTTRAPR
                     GQEKHGREYFFASEQEFDAMVEANGFVEWANVHGRRYGTSRKAIEERVAQGADVVLEI
                     DYQGALQVKQVFKNAVLIFILPPSWDELRSRLERRGEDAADVIDLRLKNAAQEMGEAC
                     KFDFVIINEVFERALFDLKTIVHAQRLKYAAQRRARAETFESLNIP"
     gene            2138..2341
                     /gene="rpoZ"
                     /locus_tag="PK518_13795"
     CDS             2138..2341
                     /gene="rpoZ"
                     /locus_tag="PK518_13795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRM50029.1"
                     /translation="MARITVEDCLEQIPNRFQLVLAATYRARMLSQGHAPKIESRNKP
                     AVTALREIAAGKVGLEMLKKVPG"
     gene            2491..4707
                     /locus_tag="PK518_13800"
     CDS             2491..4707
                     /locus_tag="PK518_13800"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRM50030.1"
                     /translation="MAASGDASAAVANAAAASFAALLEKLDYLDSTGIEQVRQAYRYA
                     DTAHLGQLRNSGEPYITHPIAVAALCASWKLDVQALMAALLHDAMEDCGITKADLIDR
                     FGAPVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRVILIKLADRTHNMRT
                     LSDMPRSKWGRISSETLEIYAPIAHRLGLNQTYRELQDLAFRHLHPWRYATLAKAVNK
                     SRSRRRDIVQKVQSDVDAAFARVGMPVRLAGREKTLYAIHQKMSLKNLSFAQVSDLYG
                     FRVVVPSVTDCYTALGVLHQMYKPAPGKFKDHIAIPKLNGYQSLHTTLVGPAGLNIEF
                     QVRTEEMHVVAEAGVAAHWLYKAQDASGAAAERMGTKWLQSLLDIQNETRDAAEFWDH
                     VKVDLFPDAVYVFTPRSQIMALPRGATAVDFAYAIHSDVGDRTTAVRINDEQVPLRTE
                     LKNGDVVEVITADDAKPNPAWLAFVRTGRARSKIRHYVKTLAQNESADLGERLLTQAL
                     RSEGMEQLPGSEEHAAIWDKLLRFTGSKTRAELMTDIGLGKRIASIVAKRLVLLLSED
                     GQRPDALLLTRERYGAHEQVSQGSITLDGSENASVQYASCCRPVPGDPIVGYLGRGEG
                     LVVHHAQCAVARKLQNKDGERFIAVDWADEPARSFEAGITVTVANGKGVLARIAAEFA
                     SCEADIVRVDMDDAMDTTDLRFVVTVRDLAHVEAVLRTLRRTQPVLRAFRVLPQLA"
     gene            complement(4714..5265)
                     /gene="greB"
                     /locus_tag="PK518_13805"
     CDS             complement(4714..5265)
                     /gene="greB"
                     /locus_tag="PK518_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HRM50031.1"
                     /translation="MSKAFTKESDNEDDEPTLPAIPVGGKNYITPQGYARLRAELLTL
                     IDDERPKVVDIVHWAASNGDRSENGDYLYGKKRLREIDRRIRFLTKRLEIAEVVDPSA
                     HAGSDQVFFGATVTYVDDEGVERTVTILGVDEADNARQQVSWISPVARALLKAREGDE
                     VALQTPAGMRSLEILSVAYPTPR"
     gene            complement(5325..5401)
                     /locus_tag="PK518_13810"
     tRNA            complement(5325..5401)
                     /locus_tag="PK518_13810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5365..5367),aa:Arg,seq:acg)
BASE COUNT         1004 a         1695 c         1772 g          959 t
ORIGIN      
        1 aggtgcagca tcttgtgctg gtgcacgatg cgcaggccgc gcagcacctc gtcgaacagc
       61 gagcgtatgg tggattcgcg gaagaccttc tccgtcttca gttcgcgcgc ggtgatgatg
      121 aagtcctgca gggacgcgcc ctccaggtgg ttcatcacca tgtaaacggt ttcgttctcg
      181 cgaaagaaat tcagcacgct caccaccgat gcgtgcgaga tctgcgccag cgagcggccc
      241 tcttcaaaaa agctcttcag gcccaggcgg tacagcgaca gtttctctgg ctgcacctgg
      301 ggcagcagtt cgccggccgt gcgcgtggcc agcgacgcgg gcaggtactc cttgatggcc
      361 acctgctggc cctcggcatc cttggccagg tacaccacgc caaagccacc tgcggcgagc
      421 ttgtgcacca cgcggtaacc gccaatgacg gtatcggggg gcagcggggc gggtttgatt
      481 tttgacatag acacgcgtgt ggggcgggag gccggataat cgccggtttg caagcaacca
      541 accaccgaag aatcccatgg cagtctacag catgaccgga tacgccagcg tgcagttcac
      601 gggcgccgcc gcaggggagg gcgggccggg caacgcgcgc cgcctgggcc tggaaatacg
      661 ctcggtcaac agccgcttcc tggacttgtc cctgcgcctg tccgacgagc tgcgtgccca
      721 ggagcccgcg ctgcgcgcgc tgctcacgca gcagctcaag cgcggcaagg tcgagctgcg
      781 cgcctttgtg gacagcgccg aattcgctgc cctcgccgaa cccacgcccc ggttgctgca
      841 gcgcgtgggc acggtgcagg acaccgtgcg ctcctggctg cctgatgccg cgcccttgag
      901 cgttgccgag gccctgcgcc tgtgtgccaa tgctggcgcc cccgagcagg actggagcgg
      961 gccgaccatg gatttggcgc agcgcgccct ggccgacatg gtggccgcac gcgagcgcga
     1021 gggcgagcgc ctggtggcca tgttgctgga tcgactcaag cagctgcgcg cgctggccga
     1081 gcaggccggc ccgttggtgc cccggctcgt ggagcaacag cgccagcgct tcatggagcg
     1141 ctggaaggag gccatgcagc tggccgaggg tgcggccgtg cccgaggccg cgcaagaccg
     1201 ggcactgacc gaggccacgg ccttcgccat ccgcatcgac gtggccgagg aggtcacccg
     1261 gctgcagtcg cacctggatg aaatacagcg cctgctgaag aagggtggcg aggtgggcaa
     1321 gcgcctggac ttcctgatcc aggagctgca ccgcgaggcc aacaccctgg gctccaagtc
     1381 cgcggccatg gagctgacgc gcatcagcgt ggatatgaag gtcctgatcg agcagatgcg
     1441 tgagcaggtg cagaacatcg aataagcaac tttgatagcg ccttgcgctt atcccttatg
     1501 gattaccccg gaaatttgtt tgtagtctca gcccccagcg gggcgggcaa gtccagcctg
     1561 gtgaaggcct tgatggagct ggatgcacgg gtgcagccct cggtgtcgca caccacgcgc
     1621 gcgccgcgcg ggcaggaaaa gcatgggcgc gaatatttct ttgcctccga gcaggaattc
     1681 gacgccatgg tcgaggccaa cggtttcgtc gaatgggcca atgtgcatgg tcggcgctat
     1741 ggcacctcgc gcaaggccat cgaggagcgt gtggcacagg gcgccgacgt ggtgttggag
     1801 atcgattacc agggcgcgct gcaggtcaag caggttttta agaatgcggt gttgatcttc
     1861 atcctgccac cgagctggga cgagttgcgc tcgcgcctgg agcggcgcgg tgaagatgcc
     1921 gccgatgtca tcgacctgcg cctgaagaac gccgcgcagg agatgggcga ggcctgcaag
     1981 ttcgacttcg ttataatcaa cgaagttttt gaacgagcgc ttttcgacct gaaaaccatt
     2041 gttcacgccc agcgcctgaa gtacgccgca cagcgtcgcg cccgggccga gaccttcgaa
     2101 tcgctcaaca tcccctgatt gtccccggag aaaaaccatg gcccgcatta ccgtggaaga
     2161 ctgccttgag cagattccca accgtttcca gctggtgctc gccgccacct accgcgcgcg
     2221 catgctcagc cagggccacg cacccaagat cgaaagtcgc aacaagccgg ccgtcaccgc
     2281 gctgcgcgag atcgccgctg gcaaggtggg cctggagatg ctgaagaaag tcccgggctg
     2341 atggcccccg gcctgttgtc caaacagcaa aaaagcaccg caccggcggt gctttttttt
     2401 gtctgactgc tggcgatcgg ccgggcaagt tacagttgcg ccatgaatgg tgggttcaac
     2461 aaggcagttg caaccggttt gccggccgga gtggcagcgt ctggcgacgc gtcagcggcg
     2521 gtggccaatg cggccgcggc cagctttgcc gcgctgctgg aaaagctcga ctatctcgac
     2581 agtaccggca ttgagcaggt gcgccaggcc taccgctatg ccgacaccgc gcacctgggt
     2641 cagctgcgca acagcggcga gccctatatc acgcatccga tcgccgtggc ggccctgtgc
     2701 gccagctgga agctggatgt gcaggcgctc atggcggcgc tgctgcacga tgccatggag
     2761 gactgcggca ttaccaaggc ggatctgata gaccgttttg gcgccccggt ggcagagctg
     2821 gtcgacggcc tgacgaaact tgacaagctg cagttcaaca cgcgcgagga gaaccaggcc
     2881 gagtcgtttc gcaagatgtt gctggccatg gcgcgcgatg tgcgcgtcat cctcatcaag
     2941 ctggcggatc gcacgcacaa catgcgcacg ctgtcggaca tgccgcgcag caagtgggga
     3001 cgcatttcgt ccgaaacgct ggagatctac gcccccattg cgcaccgcct gggcctgaac
     3061 cagacctatc gcgagctgca ggatctggcg tttcgtcacc tgcacccctg gcgctatgcc
     3121 acgttggcca aggccgtgaa caaatcgcgc agccgacgcc gtgacatcgt gcaaaaagtc
     3181 cagtccgatg tcgatgcagc ctttgcccgc gtgggcatgc cggtgcgcct ggcaggacgt
     3241 gaaaagacct tgtacgccat ccaccagaag atgagcctga agaacctgag cttcgcccag
     3301 gtgagcgacc tctacggctt tcgcgtggtc gtgccctcgg tcaccgactg ctacacggca
     3361 ctgggcgtgc tgcaccagat gtacaagccg gcgccgggca aattcaagga ccatatcgcc
     3421 atccccaagc tcaacggcta ccagtcgctg cacaccacgc tggtggggcc ggcagggttg
     3481 aacatcgagt tccaggtacg caccgaagaa atgcatgtgg tggccgaggc cggcgtggcg
     3541 gcgcactggc tgtacaaggc gcaggatgcc agcggcgccg ctgccgagcg catgggcacg
     3601 aaatggctgc agtccctgct cgacatccag aacgagacgc gtgatgctgc cgagttctgg
     3661 gaccatgtca aggtcgatct gttccccgat gcggtctatg ttttcacgcc cagaagccag
     3721 atcatggccc tgccgcgtgg cgctaccgcg gtcgatttcg cctacgccat ccacagcgac
     3781 gtgggcgaca gaaccacggc cgtgcggatc aacgacgagc aggtgccgct acgcacggaa
     3841 ctcaagaacg gcgacgtggt ggaggtcatc accgccgacg acgccaaacc caaccccgcc
     3901 tggctggcct ttgtgcgcac cggacgcgcc cgctcgaaga tccgccacta tgtgaaaacc
     3961 ttggcgcaga atgagtctgc tgatctgggc gaaaggctgt tgacccaggc gttgcgctcc
     4021 gaaggcatgg agcagctgcc tggctccgag gagcatgccg ccatctggga caagctgctg
     4081 cgctttaccg gcagcaagac ccgggccgaa ctgatgaccg acattggcct gggcaaacgc
     4141 attgccagca tcgtcgcgaa gcggctggtg ttgctgctgt cggaagatgg ccagcgtccc
     4201 gatgcattgc tgctcacgcg cgagcgctac ggcgcgcacg aacaggtgtc gcagggcagc
     4261 atcacgctgg acggcagcga gaatgcgtcg gtgcaatatg ccagctgctg tcgccccgtg
     4321 cccggcgacc ccatcgtcgg ttacctgggg cgcggcgagg ggttggtggt gcaccacgcg
     4381 caatgcgcag tggccaggaa gctgcagaac aaggatggcg agcgcttcat cgccgtggac
     4441 tgggcggacg agcctgcgcg cagctttgag gcaggtatca ccgtcacggt ggccaatggc
     4501 aagggcgtgc tggcgcgcat cgccgcagag tttgccagct gcgaggcgga catcgtgcgc
     4561 gtggacatgg acgatgccat ggataccacg gacctgcggt ttgttgtcac cgtgcgtgat
     4621 ctggcccatg tcgaggccgt gttgcgcacc ttgcgccgca cccagcccgt tctgcgggcc
     4681 ttccgagtgc tgccgcagct ggcctgactg ttttcagcgc ggtgtagggt aggccactga
     4741 caggatttcc aggctgcgca tgcccgcggg tgtttgcagc gccacctcgt cgccttcgcg
     4801 ggccttgagc agcgcgcgcg ccaccggaga aatccagctg acctgctggc gggcgttgtc
     4861 cgcctcgtcc acgccgagta tggtcaccgt gcgttcgaca ccctcgtcgt cgacataggt
     4921 cacggtggcg ccaaaaaaga cctggtcgct gcccgcatgc gcggacggat caaccacctc
     4981 ggcaatctcc agtcgcttgg tcaggaagcg gatgcgccga tcgatctcgc gtagccgctt
     5041 cttgccgtag aggtagtcgc cgttttcgga gcggtcaccg ttgctcgcgg cccaatgcac
     5101 gatgtccacc accttggggc gttcgtcgtc gatgagtgtc aggagctcag cgcgcaggcg
     5161 cgcgtagccc tggggcgtga tgtagttttt gccaccgacc ggtatggcgg gcagggtggg
     5221 ctcgtcgtct tcgttatctg actctttggt gaaggctttg ctcatgggcg catgatgcac
     5281 aaacgaaaaa gccctgaagt ctctcaactt cagggctttg cgtttggtgc ggctggcagg
     5341 aatcgaaccc acgacccctt ggttcgtagc caagtactct atccagctga gctacagccg
     5401 ccaagctttg aattgtagca cgaaaaaatc
//
LOCUS       DAOMTQ010000182         5416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3732_length_5416_cov_26.383697, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000182 DAOMTQ010000000
VERSION     DAOMTQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_5416_cov_26.383697"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..547)
                     /gene="grpE"
                     /locus_tag="PK518_13815"
     CDS             complement(17..547)
                     /gene="grpE"
                     /locus_tag="PK518_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRM50032.1"
                     /translation="MSDIQNPAPEEIEAALAANAADELARLQGELAELKTKSAELADQ
                     FLRAKAEAENARRRAEEEVAKARKFGIESFAESLLPVCDSLDAALAIESATAEQLREG
                     SDATLRQLVGALERNKVVVINPAGGDKFDPHQHQAISMVPAEQEANTVVSVLQKGYLI
                     ADRVLRPALVTVAAPK"
     gene            776..1150
                     /gene="rpsF"
                     /locus_tag="PK518_13820"
     CDS             776..1150
                     /gene="rpsF"
                     /locus_tag="PK518_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRM50033.1"
                     /translation="MRHYEIILLIHPDQSEQVPAMLERYKGMITAGGGQVHRVEDWGR
                     RQLAYLINKLAKAHYLCLNIEADQAVMAELEHAFKFNDAVLRHLTVQKKKADTGPSSM
                     MKTVEREEARKASQAEFAATER"
     gene            1163..1453
                     /gene="priB"
                     /locus_tag="PK518_13825"
     CDS             1163..1453
                     /gene="priB"
                     /locus_tag="PK518_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HRM50034.1"
                     /translation="MSNHCTLTACIAEAQPLRYTPAGLPALDLRLEHESLQREAGSER
                     QVKASVKAIAFGTLAERLARQALGSSWTFQGFLAMGRGGKGLVFHIQDIQQN"
     gene            1471..1752
                     /gene="rpsR"
                     /locus_tag="PK518_13830"
     CDS             1471..1752
                     /gene="rpsR"
                     /locus_tag="PK518_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRM50035.1"
                     /translation="MAAFKKFNKDKRPKRNTQSLLFKRKRFCRFTVAGVEEIDYKDVD
                     TLRDFIAENGKIIPARLTGTRAIYQRQLNTAIKRARFLALVPYSDQHKI"
     gene            1765..2217
                     /gene="rplI"
                     /locus_tag="PK518_13835"
     CDS             1765..2217
                     /gene="rplI"
                     /locus_tag="PK518_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRM50036.1"
                     /translation="MQIILLDKVVNLGNLGEIVKVKDGYARNFLIPTGRARRATTAAK
                     AEFEARRAELEKAAAEKLAAAQAQGEKLAGSSVKLTQKAGVDGRLFGSVTNQDIAEEL
                     NKQGYGLVKSQVRLPNGPIKTVGDNTITVSLHTDVAVEVTVSVYGETA"
     gene            2403..3818
                     /gene="dnaB"
                     /locus_tag="PK518_13840"
     CDS             2403..3818
                     /gene="dnaB"
                     /locus_tag="PK518_13840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRM50037.1"
                     /translation="MSAVLPLPIDGYLAVPPDREVAQLRVPPHSIEAESSVLGGLLLD
                     NMAWDRVGDLLVDGDFYRHEHQQIYAAIGALVNASKPADVITVYEQLQSLGKAEETGG
                     LAYLNSLAQYVPSASNIRRYAEIVRERAILRKLVTASDEIATNAFNPQGKPVERILDE
                     AEQKIFAIGEEGSRMKQGFQSLDTLVIDLLDRVQEMADNPMDVTGVPSGFVDLDRMTS
                     GLQAGDLVVLAARPSMGKTSFAVNIAEHVALNEGLPVAIFSMEMGAAQLAVRIVGSIG
                     RVNQGNLRTGKLSDEEWPRLTEAIERLRTVSLHIDETPGLTPSELRANARRLARQCGK
                     LGLIVVDYLQLMSGSGSSSSDNRATELGEISRGLKMLAKELQCPVIALSQLNRSVEQR
                     TDKRPMMSDLRESGAIEQDADIIMFIYRDDYYNKDSKEPNIAEVIIGKQRNGPTGTVK
                     LFFQKNQTRFENLAMGSGDDY"
     gene            3891..>5416
                     /locus_tag="PK518_13845"
     CDS             3891..>5416
                     /locus_tag="PK518_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRM50038.1"
                     /translation="MPDSHTYLQQVLQDLGLNLQAFAGSDCTIRAPRDGAVLAHLKWC
                     GAAQAQAQIANAQRASQTWRNVPAPVRGDLVRRLGELLRRHKAALGALVSLEAGKITA
                     EGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPMGVVGIISAFNFPVA
                     VWSWNAALALVCGNAIVWKPSEKTPLTAIACQHLLLQAMAAFNADRPGTVPEGLSQLL
                     IGGRELGELLAASAQVAVVSATGSTRMGRQVAVKVAERFGRAILELGGNNAMIVTPTA
                     DLELAARAITFAAVGTAGQRCTTLRRLIVHESVADALLARLGQIYQSIAIGDPLQEGT
                     LVGPLIDRAAFDAMQQALAQARAEGGQVLGGERVREELGQEAWYVRPALVQMPQQSAV
                     VQHETFAPILYVLRYDGDTDMAIALQNAVPQGLSSAIFTQSLMDAERFMSAAGSDCGI
                     ANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKGYMRRATNTINYSGALPLAQGVRF
                     "
BASE COUNT          980 a         1762 c         1758 g          916 t
ORIGIN      
        1 cgctcaacgc tcaacctcac ttgggcgccg ccaccgtcac cagcgccggg cgcagcacgc
       61 ggtcggcaat caggtagccc ttttgcagca cgctgaccac ggtgttggct tcctgctcgg
      121 cgggcaccat gctgatggcc tggtgctggt gcgggtcgaa cttgtcgccg ccggcggggt
      181 tgatcaccac caccttgttg cgctccagcg caccgaccag ctggcgcagc gtggcgtcgg
      241 agccctcgcg cagttgttcg gccgtggcgc tttcgatggc cagggccgcg tccaggctgt
      301 cgcacacggg cagcaggctt tcggcaaagc tttcgatacc gaacttgcgc gccttggcga
      361 cttcttcctc ggcgcggcgg cgggcgtttt cggcctcggc cttggcgcgc aggaactggt
      421 cagccagttc ggcgctcttg gtcttgagct cggccagctc gccctgcagg cgggccagct
      481 cgtcggcggc attggccgcc agtgcggctt cgatttcttc gggggctgga ttctggatgt
      541 cggacatgtc ggctagggtt ggctttgatt aatgcagctt gatgcgcggc tacatggggg
      601 cggttcggca gacttcaagc gacaagacgc cccgtcccgt tcggtgcctg tggtgtcccc
      661 ccggttggca ggcgccaagt gtacaggctt ggggatagtg gttataatgc cgggctttgc
      721 cttgccacac cgctacacaa gacgcggcgg gtggtgtcat ccaaaaggag tttgcatgcg
      781 tcactacgaa atcattttgt tgatccatcc ggatcaaagc gagcaggttc cggccatgct
      841 ggagcgctac aagggcatga tcaccgccgg cggtggtcag gtgcaccgcg tggaagattg
      901 gggtcgccgc caactggcct acctgatcaa caagctggcc aaggcccatt acctgtgcct
      961 gaacatcgaa gccgaccagg ccgtgatggc cgagctggag catgccttca agttcaacga
     1021 cgccgtgctg cgccacctga cggtgcaaaa gaagaaggcc gacaccggcc cgtcttccat
     1081 gatgaagacc gtcgagcgcg aagaggcacg caaggccagc caggccgagt ttgccgccac
     1141 cgagcgctga tcaccggagg atgtgagcaa ccattgcacc ctgactgcct gtatcgccga
     1201 ggcccagccc ctgcggtaca cgcccgccgg tctgccagcg ttggatctgc gactcgagca
     1261 tgagtcattg cagcgcgagg caggtagcga gcgccaggtc aaggccagcg tcaaggcgat
     1321 tgcctttggc actctggccg agcggctggc gcgacaggca ctcggaagca gctggacatt
     1381 ccagggcttt ctggccatgg gacgcggcgg caagggtctg gtgtttcaca tccaggatat
     1441 tcaacaaaat taacttccaa gaggcttcaa atggccgcat tcaagaaatt caacaaggac
     1501 aagcgtccta agcgcaacac ccaatcgctg ctgttcaagc gcaagcgctt ctgccgcttc
     1561 accgtcgccg gcgtcgaaga aatcgactac aaggatgtcg acaccctgcg tgacttcatc
     1621 gccgaaaacg gcaagatcat tcccgcacgc ctgaccggca cgcgcgccat ttaccagcgc
     1681 cagctgaaca ccgccatcaa gcgcgcgcgc ttcctggccc tggtgcccta cagcgaccag
     1741 cacaagatct aaggagccgg aaccatgcaa atcattctgc tcgacaaggt cgtgaacctc
     1801 ggcaacctgg gcgaaatcgt caaggtcaag gacggctacg cccgcaactt cctgatccct
     1861 acgggccgcg cccgccgcgc caccaccgcc gccaaggccg agtttgaagc gcgccgcgcc
     1921 gaactggaaa aggctgccgc cgagaagctg gccgctgccc aggcacaagg cgaaaagctg
     1981 gccggctcct cggtcaagct gacgcaaaag gcgggtgtcg atggccgcct gttcggctcc
     2041 gtcaccaacc aggacatcgc cgaagagctg aacaagcaag gctatggcct ggtgaagtcg
     2101 caggtgcgcc tgcccaatgg ccccatcaag acggtgggcg acaacaccat cacggtgtcg
     2161 ctgcacaccg acgtggccgt cgaggtcacg gtgtcggtct acggcgaaac cgcctgatcg
     2221 ccacgcaact tcggttgcgc cacgcaaaag ccgccccatg ggcggctttt gctttttgca
     2281 cgaccgcgtt ttgcacctgc ggcccaccgc ctgtgcactc cttatccaca gggttgtgca
     2341 atgacgtccg cccgcgtggc ggctagcatt tcacgtttgt ccgtatcgaa agcaatagtc
     2401 ccatgtcagc cgttctgccc ctgcccatcg atggctacct tgccgtgcca cccgaccgcg
     2461 aggtggccca gctgcgcgtg ccgccgcatt cgatagaggc cgagtccagc gtgctcggcg
     2521 gcctgttgct ggacaacatg gcctgggatc gggtgggcga cctgctggtc gatggcgact
     2581 tctaccgcca cgaacaccag cagatctacg ccgccatcgg tgccctggtc aacgccagca
     2641 agcctgccga cgtgatcacc gtgtacgagc aactgcaaag cctgggcaag gccgaggaga
     2701 cgggcggcct ggcctacctg aacagcctgg cgcagtacgt gcccagcgcc agcaacatcc
     2761 gccgctatgc cgagatcgtg cgcgagcgcg ccatcctgag aaagttggtc acggccagcg
     2821 acgagattgc caccaacgcc ttcaaccccc agggcaagcc ggtggagcgc atcctggacg
     2881 aggccgagca gaagatcttc gccatcggcg aagagggctc gcgcatgaag cagggcttcc
     2941 agtcgctcga caccctggtc atcgacctgc tggaccgcgt gcaggagatg gccgacaacc
     3001 ccatggacgt gaccggcgtg ccctcgggct tcgtcgatct ggaccgcatg accagcggcc
     3061 tgcaggccgg cgacctggtg gtgctggcgg cgcggccctc gatgggtaag acctcgttcg
     3121 ccgtgaacat cgccgagcat gtggcgctga atgagggcct gcccgtcgcc atcttctcca
     3181 tggaaatggg cgcggcccaa ctggccgtgc gtatcgtggg ctccatcggc cgcgtgaacc
     3241 agggcaactt gcgcaccggc aagctcagcg acgaggaatg gccgcgcctg accgaggcca
     3301 tagagcgctt gcgcacggtg tctctgcaca tcgacgagac cccgggcctg acgccgtccg
     3361 agttgcgcgc taacgcgcgc cgcctggcgc gccagtgcgg caagctgggg ctgatcgtgg
     3421 tggactacct gcagctgatg agcggctccg gctccagcag ctcggacaac cgcgccaccg
     3481 agctgggcga gatctcgcgt ggcctgaaga tgctggcgaa agagctgcaa tgcccggtga
     3541 tcgcgctgtc gcagttgaac cgatccgtgg agcagcgcac cgacaagcgc ccgatgatga
     3601 gcgacctgcg cgaatcgggc gccatcgagc aggacgcgga catcatcatg ttcatctacc
     3661 gcgacgatta ctacaacaag gacagcaagg agccgaacat cgccgaggtc atcatcggca
     3721 agcagcgtaa cggccccacg ggcacggtga agctgttctt ccagaagaac cagacaagat
     3781 tcgaaaacct ggccatgggt tcgggtgacg actattgata ctacttaatt gatagctgct
     3841 tgcgcttgat ggataagcgc tggagccttg tttgaccttg aggaggcgtc atgcccgatt
     3901 cgcacacgta tttgcagcag gtattgcagg atctgggcct gaacctccag gccttcgcgg
     3961 gcagcgactg caccatccgt gcgccgcgcg atggggcggt actggcccac ttgaaatggt
     4021 gcggtgccgc gcaggcccag gcgcagatcg ccaacgcaca gcgcgccagc cagacctggc
     4081 gcaatgttcc cgcccccgtg cgtggcgatc tggtgcgccg cctgggtgag ctgctgcgcc
     4141 ggcacaaggc ggccctgggc gccctggtgt cgctggaggc gggcaagatc actgccgagg
     4201 gcctgggcga ggtgcaggag atgatcgaca tctgcgactt cgccgtcggc ctgtcgcgcc
     4261 agctgcacgg cctgacgatt gccagcgagc ggcccggcca ccgcatgatg gaaacttggc
     4321 accccatggg cgtggtgggc atcatctcgg ccttcaactt ccctgtcgcc gtgtggtcgt
     4381 ggaatgccgc cctggccctg gtctgcggca atgccatcgt ctggaagccg tcggaaaaaa
     4441 cgccgctcac ggccatcgcc tgccagcatc tgctgctgca ggccatggca gccttcaatg
     4501 ccgacaggcc aggcaccgtg cccgagggtc tgagccagct cttgatcggc gggcgcgagc
     4561 tgggcgaact gcttgccgcc agtgcgcagg tggcggtggt cagcgccacg ggctccacgc
     4621 gcatgggccg ccaagtcgcg gtaaaggtgg ccgagcgctt tggccgcgcc atcctggagc
     4681 tgggcggcaa caacgccatg atcgtcacgc ccacggccga cctggagctg gcggcgcgcg
     4741 ccatcacctt tgccgccgtc ggcacggccg gccagcgctg caccacgctg cggcggctga
     4801 tcgtgcatga gtccgtggcc gatgcgctgc tggcgcgcct ggggcagatc tatcagagca
     4861 ttgccatcgg cgacccgctg caggagggca ccctggtcgg cccgctgatc gaccgcgccg
     4921 cgtttgatgc catgcagcag gcgctggccc aggcccgggc cgagggcggc caggtgctgg
     4981 gcggcgaacg cgtgcgcgag gaattggggc aggaagcctg gtatgtgcgc ccggcgctgg
     5041 tgcagatgcc gcagcagtcc gcggtggtgc agcacgagac cttcgcgccc atcctttatg
     5101 tgctgcgtta cgacggcgac accgacatgg ccatcgcgct gcagaatgct gtgccccagg
     5161 gtctgtcgtc tgccatcttc acgcagagcc tgatggatgc cgagcgcttc atgtccgctg
     5221 cgggcagcga ctgcggcatt gccaacgtca acatcggcac ctcgggcgcc gagatcggcg
     5281 gcgccttcgg tggcgagaag gaaaccggcg gcgggcgcga gtcgggatcg gacgcctgga
     5341 agggctatat gcgccgcgcc accaacacca tcaactacag cggcgcgctg ccgctggcgc
     5401 agggcgtgcg ttttga
//
LOCUS       DAOMTQ010000183         5389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3778_length_5389_cov_29.103487, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000183 DAOMTQ010000000
VERSION     DAOMTQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3778_length_5389_cov_29.103487"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1003
                     /gene="gap"
                     /locus_tag="PK518_13850"
     CDS             2..1003
                     /gene="gap"
                     /locus_tag="PK518_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRM50039.1"
                     /translation="MTIKIGINGFGRIGRNVLRSALQNFSDIEVVAINDLLEPDYLAY
                     MLKYDSVHGRFKGEVAVDGNTLIINGKKVRLTQERDPAQLKWNEVGADIVIESTGLFL
                     DKASAEKHLAAGAKKVLLSAPSKDDTPMFVYGVNHKSYDGQAIISNASCTTNCLAPVA
                     KVLHDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPALNKKLTGMSFRVPTSDVSVVDLTVELEKEASYDEIKAEMKAQSQGALKGVLAYT
                     EDKVVATDFVGETHTSVFDADAGIALDKTFVKVVAWYDNEWGYSNKCLEMVRVMAGK"
     gene            1224..2849
                     /locus_tag="PK518_13855"
     CDS             1224..2849
                     /locus_tag="PK518_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM50040.1"
                     /translation="MRINLPVSGQEYPFPSGHTLVSTTDTKGRILYCNPMFIEVSGYS
                     REELLGQPHNIVRHPDMPEEAYRDMWQTIAAGRPWSAPVKNRRKNGDYYWVMANATPL
                     LENGRPIGYMSVRTEATREQIAAAERLYATMRTEKESGRCMHQLSSGRPIRRTLGGRV
                     HEMLRLGLSAKLLLASFANIGMGYLLGHFLSPALGLGLLALVILFAWNLKVGLVARPL
                     TALIATANRLAAGDLAQTMECNRSDELGELQQALNQLSVNLCSIVRDARDQSSSMVLG
                     TTEIAQGNHNLSQRTEAQAANLEQTAAAMEQITGTVQHTADSAHQASQLTAQTRNIAE
                     RSNQAVDEVGQTMRAIQDASRRIGDITQVIDSIAFQTNILALNAAVEAARAGEHGRGF
                     AVVAAEVRALAQRSAQSAREIKQLTEDSARTVQAGHDTTEVARRTMAEVVTAVRQVNA
                     LVDEISHAAGEQVGGISQVNQAVAQLDGITQQNAAMVEQMAAAAQSLRNLAEATSQTV
                     QVFRLKGASAADAPDAVALRRRHKAQASLALQA"
     gene            2925..4307
                     /locus_tag="PK518_13860"
     CDS             2925..4307
                     /locus_tag="PK518_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRM50041.1"
                     /translation="MEYYQQAFDSSPDGLLVVDPQGLIVQANAQACTLFGYASGELDQ
                     QMIEALVPKHRRRQHIGHREHYGESPHPRAMGGQGLQLAGLRKDGSEFSVDVMLSPLR
                     GNIGPMTLCVVRDVTERVHTERLMRDLLESAPDAMVIVNAQGRIELVNSQTETVFGFA
                     RQELLGQPIEVLIPQRFRAHHPQYRSGYHANPRVRPMGEELQLYGLRKDGSEMPIEIS
                     LSPLKTSNGTLVVSAIRDISSRKRAEQLILDSLKEKEVLLKEIHHRVKNNLAVISSLF
                     SLQSNNLDDEHLKTILRQSQDRVRSMALVHETLYSHNLAEVDFTEYVTSLSAQLLTNY
                     SLSTGQVQLVTELEPILLSIDMAVPCGLILNELITNALKHGLQDGRAGRLLVALRRTE
                     PGRFELEVSDDGCGLPPDFDLASGNSLGKRLIRSLVKQLNGQVQFTSAQPGTRVLLTL
                     PLPARKESGT"
     gene            4304..>5389
                     /locus_tag="PK518_13865"
     CDS             4304..>5389
                     /locus_tag="PK518_13865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM50042.1"
                     /translation="MSPSADITPYRLLIVEDEALIAEETADRLTRLGYQVLGIADTGP
                     QAIAMAQQLQPDLVLMDIRLKGAMSGIEAAEQIYQSLQTPVIFASAHSDRETLQRAQI
                     NAQFGYIVKPFRENDLTMALRVALHRHQVEESLRSSHITHASILASINDCVVVANAQG
                     RVGFMNLPAEELLGCAGGDCFDKPLADIVPLLDAHGRQSITIDKNLPDGTTCLLNNRH
                     GQELHVEVTVTPIADTTQREIGTTVVLKDVTERKRQDALIWRQAHFDELTGLPNRAMF
                     REQLQGAMHKAAGLQGSVALLLLDLDNFKEVNDTLGHARGDELLRQVAQRIEACVRQG
                     DLVARLGGDEFAIILPGMAETTAIAQLA"
BASE COUNT         1112 a         1806 c         1659 g          812 t
ORIGIN      
        1 catgaccatc aagattggca tcaacggctt cggccgcatc ggccgcaacg tgctgcgctc
       61 ggcgctgcag aacttctccg acatcgaagt cgttgccatc aacgacctgc tggagcccga
      121 ctacctggcc tacatgctca agtacgacag cgtgcatggc cgtttcaagg gcgaagtcgc
      181 ggtggacggc aacaccctca tcatcaacgg caagaaggtt cgcctgacgc aggagcgcga
      241 ccccgctcag ctcaaatgga acgaggtcgg tgccgacatc gtgatcgagt ccaccggcct
      301 gttcctggac aaggccagcg ccgagaagca cctggccgcc ggcgccaaga aggtgctgct
      361 gtcggccccc tccaaggacg acacgccgat gttcgtctac ggtgtgaacc acaagagcta
      421 cgacggccag gccatcatca gcaacgccag ctgcaccacc aactgcctgg cccccgtggc
      481 caaggtgctg catgacaagt ggggcatcaa gcgcggcctg atgaccaccg tgcacgccgc
      541 caccgccacg caaaagaccg tggacggccc gtccaacaag gactggcgcg gcggccgcgg
      601 cattctggag aacatcatcc ccagcagcac cggcgccgcc aaggccgtgg gcgtggtgat
      661 cccggcgctg aacaagaagc tgaccggcat gagcttccgt gtgcccacct ccgacgtgtc
      721 ggtggtggac ctgacggtgg agctggaaaa ggaagccagc tacgacgaga tcaaggccga
      781 gatgaaggcc cagtcgcagg gcgcgctcaa gggcgtgctg gcctataccg aggacaaggt
      841 agtcgccacc gacttcgtcg gcgagaccca cacctcggtg ttcgacgccg acgccggcat
      901 cgccctggac aagaccttcg tcaaggtcgt ggcctggtac gacaacgaat ggggctactc
      961 caacaagtgc ctggagatgg tgcgcgtgat ggccggcaag taagccacca ctacaacccc
     1021 atccaagaac gcgccctcgg gcgcgttttt tcatgtcaat cagggtaacc cccccccacc
     1081 acaaaatcgc aacaaatatt tacataaatc tcgaatgcga caaaatcacc tgccttgact
     1141 tgagttagca aacgccaccg caccgggttc ccacccagtg ccctcgacca tcccgccatc
     1201 ccgtgccctt gtgaggtcca cccatgcgca tcaacctgcc cgtttccggc caggaatacc
     1261 ccttccccag cggccatacc ctggtttcga ccaccgacac caaggggcgc atcctctact
     1321 gcaacccgat gttcatcgag gtcagtggtt actcgcgcga ggagctgctg ggccagccgc
     1381 acaacatcgt gcgccaccct gacatgcccg aggaggccta tcgcgacatg tggcagacga
     1441 ttgccgccgg ccggccctgg tcggccccgg tgaagaaccg gcgcaagaac ggcgactatt
     1501 actgggtgat ggcgaatgcg acgccgctgc tggaaaacgg tcggcccatt ggctacatgt
     1561 cggtacgcac cgaggccacg cgcgagcaga ttgccgccgc cgaacggctc tacgcgacca
     1621 tgcgcacgga aaaggagagc ggccggtgca tgcaccagct gtccagcgga cgacccattc
     1681 ggcgcacgct ggggggcagg gtgcacgaaa tgctgcgcct gggactgtca gccaagctcc
     1741 tgttggcctc gttcgccaac atcggcatgg gctacctgtt ggggcatttc ctgagcccgg
     1801 ccctgggcct gggcctgctg gcactggtga ttctgttcgc ctggaatctc aaggtaggcc
     1861 tggtcgcccg tcccctgaca gcactgatcg cgacggccaa ccgactggcc gccggcgacc
     1921 tggcgcagac catggagtgc aaccgcagtg acgaactcgg ggaactgcag caggcgctga
     1981 accagctcag cgtgaacctg tgctccatcg tgcgcgatgc gcgcgatcag agcagctcca
     2041 tggtgctcgg caccaccgag attgcccagg gcaaccacaa cctgtcgcag cgcaccgagg
     2101 cccaggccgc gaacctggag cagaccgcgg ccgccatgga gcagatcacc ggcacggtgc
     2161 agcacacggc ggattcggcc caccaagcga gccagctgac ggcacaaacc cgcaacatcg
     2221 ccgagcgcag caaccaggcc gtcgacgagg taggccaaac catgcgtgct attcaggacg
     2281 cgtcgcggcg cattggcgac atcacccagg tcatcgactc catcgccttc cagaccaata
     2341 tcctggccct caatgccgcc gtggaggctg cgcgcgctgg cgagcatggg cgcggctttg
     2401 ccgtggtggc cgccgaggtg cgcgccctgg cgcagcgcag cgcccaatcg gccagggaga
     2461 tcaagcaact cacggaggac tccgcgcgta ccgtgcaggc cggccatgac accaccgagg
     2521 tggcgcgccg caccatggcc gaggtggtga ccgcggtgcg ccaggtgaat gccctggtcg
     2581 atgaaatcag ccatgcggca ggtgaacagg tcggcggaat ctcgcaagtg aaccaggccg
     2641 tggcgcagct cgatggcatt acccagcaga acgccgccat ggtggagcag atggcggctg
     2701 cggcgcagtc gctgcgcaac ctggcggagg ccacgagcca gaccgtgcag gtattccgcc
     2761 tcaaaggcgc gagcgcggcc gacgcccccg acgccgtggc cctgcggcgc cggcacaagg
     2821 cgcaggcaag cctggccttg caggcgtagt gtccgaaagg atccattgcc aaaccagtac
     2881 cgcagcccga tatcgcggct gcctcaaggg gagaggaagc acatatggag tactaccagc
     2941 aggcattcga ctcatcgccc gacgggctcc tggtggtcga tccacagggg ctcatcgtgc
     3001 aagccaacgc ccaggcctgc accttgtttg gctatgcatc aggcgagctt gatcagcaaa
     3061 tgatcgaagc cctggttccc aagcaccgca gacgacagca catcggtcac cgtgagcact
     3121 acggtgagtc cccccacccg cgcgccatgg gtggccaggg actgcagttg gcgggtctgc
     3181 gcaaggacgg cagcgaattt tccgtggacg tcatgctcag ccccctgcgc ggcaacatcg
     3241 gccccatgac cttgtgcgta gtccgcgacg tgaccgagcg cgtgcacacc gaaaggctga
     3301 tgcgcgacct gctggagtcg gcaccggacg ccatggtcat agtcaacgca cagggcagga
     3361 tagagttggt caactcgcaa accgaaaccg tgtttggctt tgcacggcag gagcttctgg
     3421 gccagccgat cgaggtgctg atcccgcagc gctttcgcgc ccaccacccc cagtaccgaa
     3481 gtggctacca tgccaatccg cgggtgcggc ccatggggga agaacttcag ctctatggcc
     3541 tgcgcaagga cggctcggag atgcccatcg aaatcagcct cagccccttg aagaccagca
     3601 acggcacgct ggtcgtgagc gccattcgcg acatcagcag ccgcaagcgc gcagagcaac
     3661 tgatactcga ctcactcaag gaaaaggaag tcctgctcaa ggagatacac catcgggtaa
     3721 agaacaacct ggcggtgata tccagcctgt tttcgctgca gtccaacaac ctggacgacg
     3781 agcacctgaa aaccatactg cgccaaagcc aggatcgggt gcgctcaatg gccctggtac
     3841 acgagacctt gtacagccac aacctggccg aggtggactt taccgaatat gtaaccagcc
     3901 tgagcgccca gctcctcacc aactacagcc tgagcaccgg ccaggtacag ctcgtcaccg
     3961 agctggagcc catcttgctc agcatagaca tggccgtgcc ctgcgggctg atcctcaacg
     4021 agctgatcac caatgccctc aagcatggcc tacaagacgg acgcgcgggc cggttgctgg
     4081 tggcgttgcg ccggacagag cctggacgtt tcgagctgga ggtgagcgac gatggctgcg
     4141 gcctgccgcc ggattttgac ctggccagtg gcaactcgct gggcaagcgc ctgatacgct
     4201 cgttggtcaa gcagctcaat ggccaggtgc agttcacgtc agcgcagccc ggcacaagag
     4261 tgttgctgac actacctctg cccgcacgca aggaatccgg tacatgagcc ccagtgccga
     4321 catcaccccc tatcggctgc tcatcgtcga ggacgaggcc ctgattgccg aggaaaccgc
     4381 cgaccgcctc acccgcctgg gctaccaagt gctgggcatt gccgacacgg ggccccaagc
     4441 catcgccatg gcgcagcagt tacaaccaga cttggtgctg atggacatac gcctgaaagg
     4501 cgccatgagc ggcatagagg cggccgagca gatctaccaa agcctgcaga cgccggtgat
     4561 cttcgcctcg gcgcattccg accgggagac cctgcagcgc gcgcagatca atgcgcagtt
     4621 cggctacatc gtgaagccgt ttcgcgagaa cgacctgacg atggcgctgc gcgttgcgct
     4681 gcaccgccac caggtggagg aaagcctgcg ctcgtcccac atcacgcatg cctccatcct
     4741 cgccagcatc aacgactgcg tggtggtggc caacgcccag gggcgtgtgg gcttcatgaa
     4801 cctgccggcc gaggagctgc tgggctgcgc cggcggggac tgtttcgaca agccactggc
     4861 ggacatcgtg ccgctgctgg acgcccatgg ccggcaaagc atcaccatcg acaagaacct
     4921 gcccgacggc accacctgcc tgctgaacaa ccgccacggg caggagctgc atgtcgaggt
     4981 caccgtcacg ccgatagccg acaccacgca gcgcgagatc ggcaccaccg tggtgctcaa
     5041 ggacgtgacc gagagaaaac gccaggacgc gctgatctgg cgccaggcgc atttcgacga
     5101 actgaccggc ctgcccaacc gcgccatgtt ccgtgaacag ctgcagggcg ccatgcacaa
     5161 ggccgccggc ctgcagggca gcgtggcgct gctgctgctg gatctggaca acttcaagga
     5221 ggtcaacgac accctgggcc acgcccgggg cgacgagctg ctcaggcagg tggcgcagcg
     5281 catcgaggcc tgcgtgcgcc agggcgacct ggtggcgcgc ctgggcggtg acgagttcgc
     5341 catcatcctg cccggcatgg ccgagaccac ggccattgcg cagctggcc
//
LOCUS       DAOMTQ010000184         5377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3787_length_5377_cov_27.196167, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000184 DAOMTQ010000000
VERSION     DAOMTQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3787_length_5377_cov_27.196167"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PK518_13870"
     CDS             complement(<1..667)
                     /locus_tag="PK518_13870"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM50043.1"
                     /translation="MTASASSHEAWYPNVEKTSQTDDQRIEDITVLPPPEHLIRFFPI
                     RGTEVESLIGDTRRNIQRIMSGEDDRLLVILGPCSIHNPQAALEYAQKLKAVRAQYKD
                     QLEVVMRVYFEKPRTTVGWKGLINDPYLDGSYRIDEGLRIARQLLIDINRMGVPAASE
                     FLDVISPQYIGDLISWGAIGARTTESQVHRELASGISAPIGFKNGTDGNIRIATDAIQ
                     SASR"
     gene            complement(926..2386)
                     /gene="tldD"
                     /locus_tag="PK518_13875"
     CDS             complement(926..2386)
                     /gene="tldD"
                     /locus_tag="PK518_13875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HRM50044.1"
                     /translation="MISREPTIERLALARELLLTPFGLDESHLSRALAEIRAHQVDDA
                     DLYFQYTRAEGWSLEEGIVKTGSFSIDQGVGVRAVSGEKTAFAYSDDISQASLLDAAR
                     TVRSISAAAKGGRARVASKKIAKSRSLYPHIDPIASMDSTAKVQLLERAEQRARAKDP
                     RVVQVMAGLASEYDVVLVARADGTLAADVRPLVRLSITVIAEQGGRREVGSAGGGGRF
                     GLAYFSDEQIGKYVDEAVNAALVNLESRPAPAGEMTVVLGPGWPGVLLHEAVGHGLEG
                     DFNRKGSSAFSGRIGQRVAAKGVTVLDDGTIADRRGSLNVDDEGHATQRNVLIEDGIL
                     KGYIQDAMNARLMGAQPTGNGRRESYAHIPMPRMTNTYMLAGDKDPQEIVASIKKGLY
                     ATNFGGGQVDITSGKFVFSASEAYWVENGKILYPVKGATIVGSGPESLKKVSMIGNDM
                     RLDSGVGTCGKEGQSVPVGVGQPTLRIDGITVGGTA"
     gene            complement(2439..3626)
                     /gene="rodA"
                     /locus_tag="PK518_13880"
     CDS             complement(2439..3626)
                     /gene="rodA"
                     /locus_tag="PK518_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRM50045.1"
                     /translation="MSTSAFDTPSLPQRILPLFRGFDWPLIGLLLLLSGIGLVAMYSS
                     GFDHGTRFYDHGRNMLLAAGILFVVAQVPPQRLMVLAVPLYALGVALLVAVALFGITK
                     KGATRWINVGVVIQPSEIMKIAMPLMLAWWFQKREGQLRAAEFIVAGVLLLIPVGLIM
                     KQPDLGTSLLVMAAGLSVIFFAGLPWKLVAPPVLLGALGIALIVWYEPQLCADGVRWS
                     VLRDYQQQRICTLLDPTRDPLGKGFHIIQGMIAIGSGGVWGKGFMSGTQTHLEFIPER
                     TTDFIFAAFSEEFGLAGNLVLICCFILLVWRALVIAAHAGTLFGRLMAGAVAVIFFVY
                     SFVNMGMVSGILPVVGVPLPFVSYGGTAMVTLGLALGVLMSVARAQQQPEEDPPDTFQ
                     GGL"
     gene            3660..4250
                     /locus_tag="PK518_13885"
     CDS             3660..4250
                     /locus_tag="PK518_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HRM50046.1"
                     /translation="MTTTHLLYLHGFRSSPQSAKARLTAERVAARHPGVFFWCPQLPP
                     SPRAAMALVAAGIAHWPQGTGMAVIGSSLGGYYASWVASQARCKSVLLNPAVQPARDL
                     ARHIGEQTSWHDPEERFFFRPEFVDELQALDVSHLPPAAPELLIAAQGDELLDWREMV
                     ARYPQAQQRVLEGGDHALSSFPQQLAEVLRFCDLAP"
     gene            4323..>5377
                     /locus_tag="PK518_13890"
     CDS             4323..>5377
                     /locus_tag="PK518_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRM50047.1"
                     /translation="MHALFDDAGKFLAGRILSETDSSAQIELDSGKRVKAKGANILLK
                     FDKPAPAELLAQARDIAAGIELPLAWEFAPEDEFGFADLAREYFSANCPVTEQAGMLL
                     ALFDAPHYFRRAGKGRFKKAPADILAQALAGIEKKKQLQAQIDGWAAELAAGSCPQPI
                     REQLYKILFRPDKNTPEYKAVVEASRAAHRAPLDLLQAAGAIDSPYQFHWKRFLFENF
                     PKGTAFPPLAAPQPPQDLPLADVQAYSIDDSQTTEIDDALSVRGLGTGQVTVGIHIAA
                     PGLAIQPGGDLDKLGRQRLSTVYMPGYKITMLPDEVVQIYTLDEGRANPAVSLYVTVD
                     EATLAITASETRLERVPV"
BASE COUNT          916 a         1821 c         1777 g          863 t
ORIGIN      
        1 cgcggctggc ggactggatg gcgtcggtgg cgatgcggat gttgccgtcg gtgccattct
       61 tgaagccgat gggcgccgag atgccggagg ccagctcgcg atgcacctgg ctttcggtgg
      121 tgcgcgcgcc gatggcgccc cagctgatca ggtcgccgat gtactgcgga ctgatcacgt
      181 ccagaaattc gctcgctgcc ggcacgccca tgcggttgat gtcgatgagc agctggcgcg
      241 cgatgcgcag gccctcgtcg atgcggtagc tgccatccag gtaggggtcg ttgatcaggc
      301 ccttccagcc cacagtggtg cgcggcttct cgaagtagac gcgcatcacc acctcgagct
      361 gatccttgta ctgcgcgcgc accgccttga gcttttgcgc gtattccagc gccgcctggg
      421 ggttgtggat ggagcagggg ccgaggatga ccagcaggcg gtcgtcctcg ccgctcatga
      481 tgcgctggat gttgcggcgc gtgtcgccaa tcagcgattc gacctcggtg ccgcgaatgg
      541 ggaagaagcg gatcagatgt tcgggagggg ggagcacggt gatgtcctcg atgcgttggt
      601 cgtcggtctg gctggttttc tcgacgttgg gataccaggc ttcgtggctc gaggcagatg
      661 cggtcatggg gcgatccttg tgggtggata gggcgtaggt cgggacaggt ttgaaaacaa
      721 aaaaaaccgc cgggctttgc agcttcggcg gttcttgtga gaagggggct cggtcagggt
      781 gcgcgtctac ctctctcatc cgccatggac agagaaccaa aagtaaaagt aaaacgcgct
      841 gtggactggc atggctttca atgtagcatg atttgcggca ctgcaacaaa accatggtgt
      901 agccatggcg ccacaggttt tggcatcagg ccgtgccccc gacggtgatg ccgtcgatgc
      961 gcagcgtggg ctggcccacg cccacgggca cgctctgccc ctccttgccg caggtgccaa
     1021 cgccgctgtc aaggcgcatg tcgttgccga tcatgctcac ttttttcagc gactcggggc
     1081 cgctgccgac tattgtggcg cccttgacgg ggtagagaat cttgccgttt tccacccaat
     1141 aggcctcgct ggccgagaac acgaacttgc cgctggtgat gtccacctgg ccgccgccaa
     1201 aattggtggc gtacagcccc ttcttgatgc tggcgacgat ttcctgcgga tccttgtcgc
     1261 cggccagcat gtaggtgttg gtcatgcgcg gcatgggaat gtgggcatag ctctcgcgcc
     1321 ggccgttgcc ggtgggctgg gcgcccatca ggcgcgcgtt cattgcgtcc tggatatagc
     1381 ccttcaggat gccatcctcg atcaacacat tgcgctgcgt ggcgtggcct tcgtcgtcca
     1441 cgttgagcga gccgcgccgg tcggcaatgg tgccgtcgtc cagcaccgtg acgcccttgg
     1501 ccgccacgcg ctggccaatg cggccggaaa acgccgacga acccttgcgg ttgaagtcgc
     1561 cctccagccc atggcccacg gcctcgtgca gcagcacgcc gggccagccc gggcccagca
     1621 ccacggtcat ctcgccggcc ggcgcggggc gcgattccag gttcaccagg gcggcgttga
     1681 cggcctcatc cacatatttg ccgatctgct cgtcgctgaa ataggccagg ccgaagcgcc
     1741 cgccgccgcc ggccgagccc acctcgcggc gcccgccctg ctcggcgatc acggtgatgg
     1801 acaggcgcac cagcgggcgc acgtcggccg ccagggtgcc gtctgcgcgc gccaccagca
     1861 ccacgtcgta ctcgctggcc aggcccgcca tgacctgcac cacgcgcggg tccttggcgc
     1921 gggcgcgctg ctcggcgcgc tccagcagct gcaccttggc cgtgctgtcc atggacgcta
     1981 tggggtcgat gtgcggatag agggagcggc tttttgctat ctttttagaa gctactcgcg
     2041 cgcgcccacc cttggctgca gccgaaattg accgcacggt gcgggcagca tcgagcagcg
     2101 aggcctggga gatgtcgtcg gaatacgcaa aggccgtctt ctcgccgctg acggcgcgta
     2161 cgcccacgcc ctggtcgatg gaaaacgacc cggtcttgac gatgccctcc tccaggctcc
     2221 agccctcggc gcgcgtgtac tgaaagtaca ggtcggcgtc gtcaacctgg tgcgcgcgga
     2281 tttccgccag cgcgcgggag aggtgggatt catccaggcc gaagggcgtg agcagcagct
     2341 cgcgggccag ggccaggcgt tctatggtgg gttcgcggga gatcatgggg cgattgtagg
     2401 caggcgccgc caaccctacg cccggtctgg gccggcggct acagcccgcc ctggaaggtg
     2461 tctggcggat cctcttcggg ctgttgctgg gcgcgcgcca ccgacatcag cacccctagc
     2521 gccagcccca gcgtgaccat ggccgtgccg ccatagctga caaacggcaa tggcacacca
     2581 accacgggca ggatgccgct gaccatgccc atgttcacga acgagtagac aaagaagatc
     2641 accgccacgg cgccggccat caggcggcca aacagcgtgc cggcgtgggc ggcgatcacc
     2701 agggcgcgcc acaccagcag gatgaagcag cagatcagca ccaggttgcc cgccaggccg
     2761 aactcttcgg agaaggcggc gaagatgaag tcggtggtgc gctcgggtat gaactccaga
     2821 tgcgtctgcg tgcccgacat gaagcccttg ccccagaccc cgcccgagcc tatggcgatc
     2881 atgccctgga tgatgtgaaa gcccttgccc agcgggtcgc gcgtggggtc cagcagggtg
     2941 cagatgcgtt gctgctggta gtcgcgcagc accgaccagc gcacgccgtc ggcgcacagc
     3001 tggggttcat accagacgat gagcgcaatg cccagcgcgc ccagcagcac cggcggcgcc
     3061 accagcttcc agggcaggcc ggcaaagaag atcaccgaca ggccggcggc catgaccagc
     3121 agcgaggtgc ccagatccgg ctgcttcatg atcagcccca cgggaatcag cagcagcacg
     3181 ccggcgacga tgaactccgc cgcgcgcagc tgcccctcgc gcttctggaa ccaccaggcc
     3241 agcatcagcg gcatggcgat cttcatgatt tcactcggct gtatcaccac gcccacgttg
     3301 atccagcgcg tggcgccctt cttggtgatg ccgaacagcg ccaccgccac cagcagcgcc
     3361 acgcccagcg catacagcgg cacggccagc accatcaggc gctgcggcgg cacctgcgcc
     3421 accacgaaca gaatgcccgc cgccagcagc atgttgcgcc catggtcata aaagcgcgtg
     3481 ccatggtcga agcccgacga atacatggcc accaggccta tgcccgacag cagcagcagc
     3541 agcccgatca gcggccagtc gaagccacgg aacaggggca ggatgcgctg cggcagggac
     3601 ggcgtgtcga aggcggaagt ggacatgggg cgcaattatc ggcggccaca taatccccca
     3661 tgaccaccac ccatctgctc tacctgcacg gctttcgctc ctcaccccag tccgccaagg
     3721 cccgcctgac ggccgagcgc gtggccgccc gacacccagg cgtgttcttc tggtgcccgc
     3781 agctgccgcc ttcgccgcgc gcggccatgg cgctggtggc ggccggcatc gcccattggc
     3841 cccagggcac gggcatggcg gtgatcggct cgtcgctggg cggctactac gccagctggg
     3901 tggcaagcca ggcacgctgc aagagcgtgc tgctcaaccc cgccgtgcag ccggcgcgcg
     3961 acctggcgcg ccatattggc gagcagacca gctggcatga cccggaggaa cgctttttct
     4021 tccgccccga attcgtggac gagttgcagg cgctggacgt gagccacctg cccccggccg
     4081 cgcccgagct gctgatcgcc gcccagggcg acgaactgct ggactggcgc gagatggtgg
     4141 cgcgctaccc gcaggcgcag cagcgcgtgc tggagggtgg cgaccatgcg ctgagcagct
     4201 tcccccagca gctggccgag gtgctgcgct tctgcgacct ggccccgtag accctgcagg
     4261 atcgacccgc cggcggtgac agtccccggg gccggcacgc cggcatggga aaatcgccgg
     4321 ctatgcatgc actgttcgac gacgccggca aattcctcgc cggccgcatc ctctccgaaa
     4381 ccgacagctc ggcgcagatc gagctggact ccggcaaacg cgtcaaggcc aagggcgcca
     4441 acatcctttt gaagttcgac aagccggccc ccgccgagct gctggcgcag gcgcgcgaca
     4501 tcgccgccgg catcgagctg ccactggcct gggaattcgc ccccgaggac gagttcggct
     4561 ttgcggatct ggcgcgcgag tacttctctg ccaactgccc ggtgaccgag caggccggca
     4621 tgctgctggc gctgtttgat gcgccgcatt acttcaggcg cgccggcaag ggccgcttca
     4681 agaaggcccc ggccgacatc ctggcccagg cgctggcggg tatcgagaag aagaagcagc
     4741 tccaggcgca gatcgacggc tgggccgcag aacttgcggc cggcagctgc ccgcagccga
     4801 tacgcgagca gctctacaag atcctgttcc gcccggacaa gaacacgccc gagtacaagg
     4861 ccgtggtcga ggccagccgc gccgcgcaca gggcgccgct cgatctgctg caggccgcgg
     4921 gcgccatcga ctcgccctac cagttccact ggaagcgctt tctgttcgag aacttcccca
     4981 agggcacggc cttcccacca cttgccgcgc cgcagccgcc acaggacctg ccgctggccg
     5041 acgtgcaggc ctattccatc gacgactcgc aaaccaccga gatcgacgac gccctgtcgg
     5101 tgcgcggcct gggcacgggg caggtcaccg tgggcataca cattgccgcg cccggcctgg
     5161 cgatacagcc cggcggcgac ctggacaagc tgggccgcca gcgcctgtcc acggtctaca
     5221 tgccgggcta caagatcacc atgctgcccg acgaggtggt gcagatctac accctggacg
     5281 agggccgcgc caacccggcc gtgtcgctgt acgtgacggt ggacgaggcg acgctggcca
     5341 tcaccgccag cgagacgcgc ctggagcgcg tgccggt
//
LOCUS       DAOMTQ010000185         5341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3838_length_5341_cov_28.736285, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000185 DAOMTQ010000000
VERSION     DAOMTQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3838_length_5341_cov_28.736285"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PK518_13895"
     CDS             <1..1017
                     /locus_tag="PK518_13895"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HRM50048.1"
                     /translation="SPDVTLEEDLARRDLTINAIAVGADETGVEAIFDPYDGRRDIAQ
                     RLLRHVTDAFREDPVRILRVARFAARFTDFSVAPETMQLMREMVQHGEADHLVAERVW
                     QELSRGLMEAQPSRMFAVLRECGALAVVLPELDRLWGVPQRAEYHPEVDTGVHVMMVL
                     DMAARLEAPLAVRFACLVHDLGKGTTPQDVLPRHIGHEQRSSKLLQAVAERLRVPVDC
                     RELADVVAREHGNIHRSSELGAAALVRLLERCDAIRKPARLDEILLACECDARGRLGY
                     EDAPYPQRARINAALAAVQSVVTSSIAAQAAAQGLQGAKVGERIHAARVRAVADWLGT
                     ASTH"
     gene            complement(1006..1443)
                     /gene="trxC"
                     /locus_tag="PK518_13900"
     CDS             complement(1006..1443)
                     /gene="trxC"
                     /locus_tag="PK518_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HRM50049.1"
                     /translation="MTTEALHIVCPHCHTTNRVQAAQLASSPDCGSCHRPLFTGAPLE
                     LDAASFDKHVGRSHIPVVVDFWAPWCGPCRQMAPAFAQAARDLEPGVRLAKLDTEAHP
                     AIAGRYGIRSIPTMIMFRGGQEVARVSGALGAADIARWVRSVG"
     gene            complement(1504..1698)
                     /locus_tag="PK518_13905"
     CDS             complement(1504..1698)
                     /locus_tag="PK518_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRM50050.1"
                     /translation="MLRWLIVVFLALVLINGLTPWLKRLGLGRLPGDFQFRLFGRDWF
                     IPLASTLLLSFLLSLAVKWL"
     gene            complement(1699..2097)
                     /locus_tag="PK518_13910"
     CDS             complement(1699..2097)
                     /locus_tag="PK518_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM50051.1"
                     /translation="MTLEIDRIDHIVLNVADVQASAAWYARVLGMEREVFDSRTGPRV
                     AVKFGRQKINLRPMGEDTVAWFTGANPMPGSDDLCFITAQPMRQVLDHFASVGVALEV
                     GPVERTGALGAMQSVYCRDPDGNLVEVAVY"
     gene            complement(2116..3201)
                     /locus_tag="PK518_13915"
     CDS             complement(2116..3201)
                     /locus_tag="PK518_13915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM50052.1"
                     /translation="MTTPTDALIHHIHQQITAAGGWLGFDRFMELALYTPGLGYYAGG
                     LRKLGRMPEDGSDFVTAPELSPVFGQVLAAQVREALDATGTQEVWEFGAGSGALAEQV
                     LGELGSSLRRYTIVDLSGELRARQQERLAPWGDKVAWAERLPAAIEGVVVGNEVLDAM
                     PVQLLQRTDDIWHERGVVTGQDGGFAWQDRPTDLRPPVEIEGGHDYLTEIHAQAEAFM
                     RTLAAHLKRGAAFLIDYGFGEDEYYHPQRHMGTVMCHRAHRADDDPLADVGQKDITAH
                     VNFTAMAVAAQDAGLHVLGYTSQARFLLNCGLLQKMELLTLVQRAPAAKLMLEHEMGE
                     LFKVLAVAPGEPWTPMGFAQGDRTHRL"
     gene            3370..4164
                     /locus_tag="PK518_13920"
     CDS             3370..4164
                     /locus_tag="PK518_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM50053.1"
                     /translation="MNHTDWVLVTGAAHRLGREIALAFAAAGWNVLCHYRASLAGAEG
                     TQAAVRALGRECLLVPADLARPDAATALLQQCVAVTGRAPRCIVNNASIFEADDARTA
                     SAEALLRHFQTNTATPLLLGNALHAWLIAQGEATHGQHSVIHVLDQKVHNLNPDYFSY
                     TVSKLALERSVQLQAQALAPLVRVCGLSPGLMYLSGPQTQDNFDQASRVNLLREPITP
                     LDVARCAVFIASNASLNGCTLQADNGQHLVPLARDVMFAIGENPRP"
     gene            4161..4574
                     /locus_tag="PK518_13925"
     CDS             4161..4574
                     /locus_tag="PK518_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRM50054.1"
                     /translation="MNSILLAFGALDARLATQCRALSLRRHEVPVRIGVHAHERQGAQ
                     RMWFDVDLCVKLAQAPADRDHISQTLDYDFVREVIAREVGGQHHELQETLCDAIATAL
                     LQENGVHAVRVATRKPDIYPDSASIGVERVCIKPW"
     gene            4579..4971
                     /locus_tag="PK518_13930"
     CDS             4579..4971
                     /locus_tag="PK518_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRM50055.1"
                     /translation="MTELPYHSRPGEGRQALTLTGLRFDANLGILAHEKAAPQPIQVD
                     AELHLGPQPLAPRDDDIRHVLDYRKVRQIIIDECTAEHVNLLESLIGKLAQRLMQLPG
                     VLGVRVKIAKLEIFDDCEVAIRVETGQW"
     gene            4977..>5341
                     /locus_tag="PK518_13935"
     CDS             4977..>5341
                     /locus_tag="PK518_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM50056.1"
                     /translation="MNTITENTPLQAADWIGEEPATDNRKIEREQIKLEKRLCREVGR
                     AVLDYNMIEEGDRVMVCMSGGKDSYTLLDILRKLQKRAPIKFDIVAVNLDQKQPGFPE
                     HVLPEYFKSIGVEYHIENQ"
BASE COUNT          908 a         1825 c         1797 g          811 t
ORIGIN      
        1 tcgcccgacg tgacgctgga ggaagacctg gcacggcgcg acctcactat caatgcgata
       61 gctgttggcg ccgatgagac gggcgtagag gccatttttg acccttatga cggcaggcgc
      121 gacatcgcgc agcgcctgct gcgccatgtg accgacgcct tccgcgaaga cccggtgcgc
      181 atcctgcgcg tggcgcgctt tgcggcgcgc ttcaccgatt tctcggtggc gcccgagacc
      241 atgcaactga tgcgcgaaat ggtgcagcac ggcgaagccg accatctggt agccgagcgc
      301 gtgtggcaag aactgtcgcg cgggctgatg gaggcacagc cgtcgcgcat gttcgcggtg
      361 ctgcgcgaat gtggcgcact ggccgtggtg ctgcccgagc tcgaccgtct gtggggcgtg
      421 ccccagcgcg ccgaatacca ccccgaggtg gacaccggcg tgcacgtgat gatggtgctg
      481 gacatggcgg cgcgcctgga ggcgccactg gccgtgcgct ttgcctgcct ggtgcatgac
      541 ctgggcaaag gcacgacgcc gcaggatgtt ctgccgcgcc acatcggcca cgagcagcgc
      601 agcagcaaac tgctgcaggc agtggccgag cgcctgcgcg tgcccgtgga ctgccgcgag
      661 ctggccgacg tggtggcgcg cgagcacggc aacatccacc gcagcagcga gctgggcgcg
      721 gccgcgctgg tgcgattact ggagcgctgc gacgcgatcc gcaagccggc gcggctggac
      781 gagattttgc tggcctgcga gtgcgacgca cgcgggcgcc tgggctatga agacgcgccc
      841 tacccgcagc gcgcgcgcat caacgcggcg ctggcggcag tgcagtcggt cgtcaccagc
      901 agcattgccg cgcaggccgc cgcccagggc ctgcaaggcg ccaaggtggg cgagcgcatc
      961 cacgccgcac gcgtgcgcgc cgtggccgat tggctaggca cggcctcaac ccactgagcg
     1021 cacccagcgc gcgatgtcgg ccgcgcccag ggcacccgag acgcgcgcca cctcttgccc
     1081 tccacggaac atgatcatcg tcggaatgct acgaatgccg tagcgcccgg cgatggctgg
     1141 atgcgcctcg gtatcgagct tggccaggcg cacgcccggc tccagatcgc gcgccgcctg
     1201 ggcgaaagcc ggcgccatct gccggcaggg cccgcaccag ggcgcccaga agtccaccac
     1261 cacggggatg tggctgcgcc ccacatgctt gtcgaagctg gcagcgtcca gctccagggg
     1321 cgcgccggtg aacagcggcc ggtggcagct gccgcaatcg gggctgcttg ccagctgcgc
     1381 ggcctgcacg cgattcgtgg tgtggcaatg ggggcagacg atgtgcaggg cttctgtggt
     1441 catggctgtg ggtttgcaac gtgttgccag cctaactgcg gccagatcgc acggatttca
     1501 agctcagagc cacttcaccg ccaggctcag caaaaaactc agcagcagcg tgctggccag
     1561 cggaatgaac caatcgcgcc cgaacagccg aaactggaag tcccccggca gccgccccag
     1621 ccccaggcgc ttgagccagg gcgtgagccc gttgatcagc accagggcca gaaagacgac
     1681 gatgagccag cgcagcattc agtaaacggc aacttcgacg aggttgccgt ccgggtcgcg
     1741 gcaatagacc gactgcatgg cgcccaacgc ccccgtgcgc tccacgggcc ctacctccaa
     1801 cgcaacgcct acgctggcga aatgatccag cacctgccgc ataggctggg cggtaatgaa
     1861 acacaggtcg tcactgcccg gcatggggtt ggcgccggtg aaccaggcca cggtgtcctc
     1921 gcccatgggg cgcaggttga tcttctggcg gccgaacttc accgccacgc gcgggccggt
     1981 gcgcgagtcg aacacctcgc gctccatgcc cagcactctg gcataccagg cggcgctggc
     2041 ctgcacgtcg gcgacgttga ggacgatgtg gtcgatgcgg tcgatttcga gggtcatggg
     2101 gtcatccttt ccagatcaca gcctgtgcgt gcggtcgccc tgggcaaagc ccatgggcgt
     2161 ccagggctcg cccggcgcca cggccagtac cttgaacagc tcgcccatct catgttccag
     2221 catcaatttg gccgctggcg cacgctggac aagcgtcaac agctccattt tttggagcaa
     2281 accgcaattg agcagaaagc gcgcctggct ggtgtagccc agcacgtgga ggcccgcgtc
     2341 ctgcgccgcc acggccatgg ccgtgaagtt gacgtgggcg gtgatgtcct tctggcccac
     2401 gtcagccagt gggtcgtcgt ccgcgcggtg ggcgcggtgg cacatcaccg tgcccatgtg
     2461 gcgctggggg tggtagtact cgtcctcgcc aaagccgtag tcgatgagaa aggccgcgcc
     2521 gcgcttcagg tgcgcggcca gggtgcgcat gaaggcctcg gcctgcgcat ggatctcggt
     2581 caggtagtcg tgcccgcctt cgatctcgac gggggggcgc agatcggtgg gccggtcctg
     2641 ccaggcaaag ccgccatcct gccccgtgac cacgccgcgc tcgtgccaga tatcgtccgt
     2701 gcgctgcagc agctgcacgg gcatggcgtc cagcacctcg ttgccgacga ccacgccctc
     2761 gatggccgcg ggcaggcgct cggcccaggc caccttgtcg ccccagggcg caaggcgctc
     2821 ctgctggcgc gcgcgcagct cgcccgacag atcgacgatg gtgtagcggc gcaggctgct
     2881 gcccagctcg cccaggactt gctcggccag cgcgccgctg ccggcgccga attcccagac
     2941 ctcctgcgtg ccggtggcgt ccagcgcctc acgcacctgg gcggccaaca cctggccaaa
     3001 gacgggggac agttccggtg cggtgacgaa gtcgctgccg tcctcgggca tgcggcccag
     3061 cttgcgcaga ccgcccgcgt aatagcccag gccgggggtg tagagggcca gctccatgaa
     3121 gcggtcaaag cccagccagc cgccggcggc ggtgatctgc tggtggatat ggtggataag
     3181 cgcgtcggtg ggggtggtca taatttgcgg ttttgcgcgc gcgtcatgcg ccggtctgat
     3241 gagcgattgt ttcaactcat ttcccccgcg accctcgcct cggtgggggc agtggcgccc
     3301 atggcgtgcc gggctgcgcg gcatgccggg cattgtattt cccggccata gcccccaagg
     3361 agcgaccgca tgaaccacac cgactgggtc ctcgtcaccg gcgccgcgca ccgcctgggg
     3421 cgcgaaatcg ccctggcctt cgcggccgcg ggctggaatg tgctgtgcca ctaccgcgcc
     3481 tcgctcgccg gcgccgaagg cacgcaagcc gcagtgcgcg ccctggggcg cgaatgcctg
     3541 ctggtgccgg ccgacctggc gcggcccgat gccgccactg ccctgttgca gcagtgcgtg
     3601 gccgtcaccg gccgggcgcc gcgctgcatc gtcaacaacg cctccatctt cgaggccgac
     3661 gacgcgcgca cggccagcgc cgaggcgctg ctgcggcatt tccagaccaa caccgccacg
     3721 ccgctgctgc tgggcaatgc cctgcatgcg tggctgattg cgcagggcga ggccacgcac
     3781 gggcagcaca gcgtgatcca cgtgctggac cagaaggtgc acaacctgaa ccccgactat
     3841 ttctcttaca ccgtgagcaa gctggccctg gagcgcagcg tgcagctaca ggcccaggca
     3901 ctggcgccgc tggtgcgcgt atgcggcctg tcgccggggc tgatgtacct gagcggcccg
     3961 cagacccagg acaacttcga ccaggccagc cgcgtgaacc tgctgcgcga gccgattacg
     4021 ccgctggacg tggcgcgctg cgcggtgttc atcgccagca acgccagcct gaacggctgc
     4081 acgctgcagg ccgacaacgg ccagcacctg gtgcccctgg cgcgcgacgt gatgtttgcc
     4141 atcggcgaaa atccgcgccc atgaattcca tactgctcgc cttcggcgcc ctggacgcgc
     4201 gcctggccac gcaatgccgc gccttgagcc tgcgccgaca cgaggtgccg gtgcgcatag
     4261 gcgtgcatgc gcacgagcgc cagggcgcgc agcgcatgtg gttcgacgtg gatctgtgcg
     4321 tcaagctggc ccaggcgccg gcggatcgtg accatatctc gcagacgctg gactacgact
     4381 tcgtgcgcga ggtgatcgcg cgcgaggtcg gcggccagca ccatgagctg caggaaaccc
     4441 tgtgcgacgc catcgccacc gccctgctgc aagaaaatgg cgtgcacgcc gtgcgcgtgg
     4501 ccacgcgcaa gcccgacatc taccccgaca gcgcatccat cggcgtggag cgggtatgca
     4561 tcaagccctg gtgaagccgt gaccgaactt ccctatcatt cgcgccctgg cgaggggcgg
     4621 caggccctca ccctcaccgg cctgcgcttt gacgccaacc tgggcatcct ggcccacgag
     4681 aaggccgcgc cgcagccgat acaggtggat gccgagctgc atctgggccc ccagccgctg
     4741 gcaccgcgcg acgacgacat acgccatgtg ctggactacc gcaaggtgcg ccagatcatc
     4801 atcgacgaat gcacggccga gcatgtgaac ctgctggaga gcctgatcgg caagctggcg
     4861 cagcgcctga tgcagctgcc cggcgtgctg ggcgtgcgcg tgaaaatcgc caaactcgaa
     4921 atttttgatg actgcgaagt tgccattcgc gtcgaaaccg gacagtggta agccccatga
     4981 acacgatcac cgaaaacacc cccctgcagg ccgccgactg gattggcgaa gagcccgcca
     5041 ccgacaaccg caagatcgag cgcgagcaga tcaagctgga gaagcgcctg tgccgcgagg
     5101 tcggccgcgc ggtgcttgac tacaacatga tcgaggaggg cgaccgcgtc atggtctgca
     5161 tgtccggcgg caaggacagc tacacgctgc tggacattct gcgcaagctg caaaagcgcg
     5221 ccccgatcaa gttcgacatc gtggccgtga acctggacca gaagcagccc gggttccccg
     5281 agcatgtgct gcccgagtac ttcaagagca taggcgtcga gtaccacatc gagaaccagg
     5341 a
//
LOCUS       DAOMTQ010000186         5304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3887_length_5304_cov_26.692322, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000186 DAOMTQ010000000
VERSION     DAOMTQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3887_length_5304_cov_26.692322"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK518_13940"
     CDS             complement(<1..232)
                     /locus_tag="PK518_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50057.1"
                     /translation="MTDANQPDPQTPAPAATDTGGYGEGAIQILEGLEAVRKRPGMYI
                     GDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIV"
     gene            complement(343..1449)
                     /gene="dnaN"
                     /locus_tag="PK518_13945"
     CDS             complement(343..1449)
                     /gene="dnaN"
                     /locus_tag="PK518_13945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRM50058.1"
                     /translation="MIVLKATQDKVLAVLQSVSGIVERRHTLPILANVLIRKTGNALQ
                     LTTSDLEIQIRTTAELGGDTGDFTTTVGARKLIDILKTMPGDQTVSLENNQSKLILKG
                     GKSRFTLQTLPAEDFPLVQEAANFGPAFSVPQKTLKTLLSQVSFAMAVQDIRYYLNGI
                     LFVAEGKKLTLVATDGHRLAFSSADLEVEVPKQEVILPRKTVLELQRLLSDADGAIEM
                     QFAGNQAKFSFGGMEFVTKLVEGKFPDYNRVIPRNHHNIITLGRAPLLASLQRTAIMT
                     SEKFKGVRLNIEPGSLRVASNNAEQEEAQDELDIDYGGDNIEIGFNVTYLLDVLANMG
                     QDMVKLELADGNSSVVFGVPDNPHFKYVVMPMRI"
     gene            complement(1561..2982)
                     /gene="dnaA"
                     /locus_tag="PK518_13950"
     CDS             complement(1561..2982)
                     /gene="dnaA"
                     /locus_tag="PK518_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRM50059.1"
                     /translation="MNEGLHSPSHDIDNASAGQALWQACTEQLAQDLPEQQFNTWIKP
                     LTAQVAPDFSKVTLLVANRFKLDWIRAQYAGRISALLEGLYGQPVTLELALAQRESVA
                     RTYVRPAASQATEAAGNAPAAPAAEEAPNGSFRTRLNPALTFETLVEGTANRMARSAA
                     MHVAGSPGHLYNPLFIYGGVGLGKTHLVHAVGNQLLKDRPDAKVLYIHAEQFVSDVVK
                     SYQRRTFDDFKERYHSLDLLLIDDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDT
                     YPKGLANIHERLVSRFDSGLTVAIEPPELEMRVAILINKARAEHAEMPEEVAFFVAKN
                     VRSNVRELEGALRKILAYSRFNQKEVSIQLAREALRDLLSIQNRQISVENIQKTVADY
                     YKIKVADMYSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKM
                     GAERQTNTELNQQLHVLEQTLKG"
     gene            3266..3400
                     /gene="rpmH"
                     /locus_tag="PK518_13955"
     CDS             3266..3400
                     /gene="rpmH"
                     /locus_tag="PK518_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRM50060.1"
                     /translation="MKRTYQPSKTRRARTHGFLVRMKTKGGRAVINARRAKGRKRLAV
                     "
     gene            3475..3924
                     /locus_tag="PK518_13960"
     CDS             3475..3924
                     /locus_tag="PK518_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRM50061.1"
                     /translation="MQRLKTRPQFQAALAAGTAARTAHFALHRLRLAPMDMPPTGPDS
                     SPQAGQAQALFGVGAPAVWLGAMVPKRWARRAVTRNTIKRQIYAVAAGFEARMQPAAH
                     VVRLRAAFDRKHFVSATSDTLKDAVRAELQQLFAAAERRKPAVEAAP"
     gene            3921..4214
                     /gene="yidD"
                     /locus_tag="PK518_13965"
     CDS             3921..4214
                     /gene="yidD"
                     /locus_tag="PK518_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRM50062.1"
                     /translation="MMRRLLMGLVRGYRLLLSPWLGSACRFEPTCSAYSLQALEQYGA
                     AAGSYLTVRRLARCHPWCEGGIDPVPRELPRGMRLFSRLMPTEPPSSSATKHS"
     gene            4211..>5304
                     /gene="yidC"
                     /locus_tag="PK518_13970"
     CDS             4211..>5304
                     /gene="yidC"
                     /locus_tag="PK518_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRM50063.1"
                     /translation="MNDIRRTILWVIFGFSMVLLWDKWQLHNGNKATFLPSPAAVGAA
                     APAAGASAPLAGAASVPASTAPAVASIGASAVPGTPAPAAPAAREKIEVTTDVYRLTF
                     DGEGGSLVHAELLKHADMTDKAKNFVLFDESTQRVYAAQTGLIGGSFPTHKTVMTAVP
                     GPRTLKDGENELTIRFESPEQGGVKLVKTWTLKRGAYDIAVKHEVVNVGSTAVSPQLY
                     LQLVRDGNKPPGESSFYSTFTGPAVYTDAKKYQKIDFKDIKNKKFDIPSESTNGYIAM
                     VQHYFATAWLLPDGVKRELRLDSADIGANTPDCCYRVQMITPLGELAPGQSKTIDARL
                     FAGPQVETMMEKLYPGLELVKDYGWLTILS"
BASE COUNT          890 a         1654 c         1711 g         1049 t
ORIGIN      
        1 cgacgatgtc gtcgcaatgc ccggccaggg cctcgtcgat ggagttgtcc accacctcga
       61 acaccaggtg gtgcaggccg gtgccgtcgg acgtgtcgcc gatgtacatg cccgggcgct
      121 tgcgcacggc ctccagaccc tccaggatct ggatggcgcc ttcgccgtag ccgccggtat
      181 cggtggctgc aggggcgggg gtctgtggat cgggctggtt cgcgtcggtc atggaatgcc
      241 ttggggctgc ggcagcccag cgctggtggc tgggcaatac ttgaagcaaa agtgctgttt
      301 gcgcccgtct atcaagcgca aatagctata aaaatagtag tatcagatgc gcatcggcat
      361 cacgacatac ttgaaatgcg ggttgtcggg aacgccaaac accaccgagc tgttgccgtc
      421 ggccagctcc agcttcacca tgtcctggcc catgttggcc agcacgtcca gaagataggt
      481 gacgttgaag ccgatttcga tgttgtcgcc gccgtagtcg atgtcgagct cgtcctgggc
      541 ctcttcctgc tcggcgttgt tggaggccac gcgcaggctg cccggctcga tgttcaggcg
      601 cacgcccttg aacttctcgc tggtcatgat ggccgtgcgc tgcaggctgg ccaggagcgg
      661 cgcgcgcccc agcgtgatga tgttgtggtg gttgcgcgga atcacgcggt tgtagtcggg
      721 gaacttgccc tccaccagct tggtgacgaa ctccatgccg ccaaagctga acttggcctg
      781 gttgccggca aactgcatct cgatggcgcc gtcggcatcg gacagcaggc gctgcagctc
      841 cagcaccgtc ttgcgcggca ggatcacctc ctgcttgggc acctcgacct ccaggtcggc
      901 gctggagaac gccaggcggt gaccgtcggt ggccaccagg gtgagctttt tgccctcggc
      961 cacgaacagg atgccgttca ggtagtagcg aatgtcctgc accgccatgg caaacgacac
     1021 ctgggacagc agcgtcttga gcgtcttttg cggcacgctg aaggcggggc cgaagttggc
     1081 ggcctcctgc accagcggga agtcctcggc cggcagggtt tgcagcgtga agcggctctt
     1141 gccacccttc agaatcagct tggactggtt gttctccagg ctcaccgtct ggtcgccggg
     1201 catggtcttc aggatgtcga tgagcttgcg tgcgcccacg gtggtcgtga agtcgcccgt
     1261 gtcgccgccg agctcggccg tggtgcggat ctgtatctcc aggtcgctgg tggtgagctg
     1321 cagggcgttg cccgtcttgc ggatcagcac attggccaat atgggcaggg tatgccggcg
     1381 ctcgacgatg ccggacacgg attgcagaac ggcgagtacc ttgtcttgtg tggccttcag
     1441 gacaatcatg tcgtcaacct ttggtggttt tgggatgggt gcgcaaacgg cggcatgggc
     1501 atggccccct ctcggccgcc aaacatggca ttttgcctgg gaacaagggc gccttgacca
     1561 tcagcccttg agcgtctgct ccagcacatg cagttgctgg ttgagctcgg tgttggtctg
     1621 gcgctcggcg cccatcttgc gcacggcgtg cagtaccgtg gtgtggtctc tgccgccaaa
     1681 cagctcaccg atctcgggca ggcttttttg cgtcagctcc ttggccaggt acatggcgat
     1741 ctggcgcggc cgggcaatgc tggccgggcg cttcttgctg tacatgtcgg cgaccttgat
     1801 cttgtagtag tcggccaccg tcttctggat gttctcgacg ctgatctgac ggttctggat
     1861 gctgagcagg tcgcgcagcg cctcgcgcgc cagctggatc gagacctcct tctggttgaa
     1921 gcggctgtag gccaggatct tgcgcagcgc gccctcgagc tcgcgcacgt tggagcgcac
     1981 gttcttggcg acgaagaagg ccacctcctc gggcatctcg gcatgctcgg cgcgcgcctt
     2041 gttgatcagg atggccacgc gcatctccag ctcgggcggc tctatggcca ccgtcaggcc
     2101 cgagtcgaag cgcgagacca ggcgctcgtg gatgttggcc aggccctttg ggtaggtgtc
     2161 gctggtcatg acgatgtggc tcttcttggc cagcagcgcc tcgaaggcgt tgaagaattc
     2221 ttcctgcgtg cggtccttgt tggcgaagaa ctgcacgtcg tcgatcagca gcagatccag
     2281 cgagtggtag cgttccttga aatcgtcaaa ggtgcgtctt tggtaggact tgaccacatc
     2341 cgagacgaat tgctcggcat ggatgtagag aaccttggcg tcgggcctgt ccttgagcag
     2401 ctggttgccc acggcatgca ccagatgggt cttgcccagg cccacgccgc cgtagatgaa
     2461 cagcgggttg tacaaatggc cgggcgagcc cgccacatgc atcgcggccg agcgcgccat
     2521 gcggttggcc gtgccctcga ccagggtctc gaaggtcagt gcggggttca ggcgcgtgcg
     2581 aaagctgccg ttcggtgcct cctccgccgc aggggccgcg ggggcgttgc cggcggcctc
     2641 ggttgcctgc gaagcggctg gtcgaacata agtcctggca acggattcgc gctgagcgag
     2701 tgctaactcc aaggtgactg gctggccata caaaccctcc agcagggcag agatgcggcc
     2761 ggcgtactgg gcgcggatcc agtcgagctt gaagcggttg gcgaccagta gcgtcacctt
     2821 ggagaaatcg ggtgcaacct gggccgtcag aggtttgatc caggtgttga actgctgctc
     2881 gggcaggtct tgggccagct gttcggtgca ggcctgccac aaggcctggc cggcgctggc
     2941 gttgtcgata tcgtggctgg gagaatgcag tccctcgttc attgcggtgc ttgtagttgt
     3001 ggatagagga acggtaaatg gccgccgatt ttagcttgtc aaagacttat ccacaatttg
     3061 ccgcgggccg gttggcaggc aatttcaacc ccaggcattg ccagttcctg cataccctgc
     3121 aataatcgcc ggtttccctg aaggatcttt cagggtgatc aaacccgggc ggcggttttt
     3181 ctgcactgat gtgtggcgag gctgccggtc cggcgggcgc ggccgatcga agcggcgcct
     3241 gtaactgaat cttcaaggat tgaacatgaa acgtacttac cagccttcca agacccgccg
     3301 cgcccgcacc catggtttcc tggtgcgcat gaagaccaag ggtggccgcg ccgtcatcaa
     3361 cgcccgccgc gccaagggcc gcaagcgcct ggccgtctaa ggtcggcttg ccaggctttc
     3421 ggccgcttcc tggtctgcac cgccggttct aggcggggac gctgccctgc caccatgcaa
     3481 cggctgaaaa cccgccccca gttccaggcc gccttggcgg caggcacggc ggcccgcacc
     3541 gcgcattttg cgctgcaccg actcaggctt gcccccatgg acatgcctcc cacagggccc
     3601 gactcctcgc cgcaggcggg gcaggcgcag gccctgtttg gcgttggtgc gcccgccgtc
     3661 tggctgggcg ccatggtgcc caagcgctgg gcgcgccgcg ccgtgacgcg caacaccatc
     3721 aagcggcaga tctacgccgt ggccgccggt ttcgaggcgc gcatgcagcc ggccgcccat
     3781 gtggtgcgcc tgcgcgcggc ctttgaccgc aagcatttcg tcagcgccac gtcggacacc
     3841 ctcaaggacg ccgtgcgcgc cgagctgcag cagctgtttg ccgctgccga gcggcgcaag
     3901 cccgccgtgg aggccgcgcc atgatgcgcc gcctgctcat gggcctggtg cgcggctacc
     3961 ggttgctgct cagcccctgg ctgggctccg cctgccgctt cgagcccacc tgctcggcgt
     4021 actcgctgca ggcactggag caatacgggg ccgccgccgg cagctacctg acggttcggc
     4081 gcctggcgcg ctgccatccc tggtgcgagg gcgggatcga tcccgtgccg cgggagctgc
     4141 cgcgcggcat gcgcctgttc tcgcggctca tgcctaccga acccccatct tcctcagcta
     4201 caaagcattc atgaacgata ttcgccgcac catcctgtgg gtgatctttg gcttttccat
     4261 ggttttgctg tgggacaaat ggcaattgca taacggcaac aaggccacat tcttaccgtc
     4321 gcctgccgcg gtgggagccg cagccccggc agccggagcc tcggcgccgc tggccggtgc
     4381 tgccagtgtg cctgcttcca cagcgcccgc agtcgcgtcc atcggcgcct cggccgtgcc
     4441 cggaacgcct gcgccagcag cccctgcagc gcgtgaaaaa atcgaagtca ccaccgacgt
     4501 ctaccgcctg acgtttgacg gcgaaggcgg ctcgctcgtg catgccgagc tgctgaagca
     4561 cgcggacatg accgataagg cgaagaactt tgtgctgttt gacgagagca cacagcgcgt
     4621 ctacgccgca caaacgggcc tgatcggtgg cagcttcccc acccacaaaa cggtgatgac
     4681 cgccgtgcct ggcccccgta ccctcaagga cggtgagaac gagctgacca ttcgctttga
     4741 atcgcctgag caaggcggcg tcaagctggt gaagacctgg acgctcaagc gtggtgctta
     4801 cgacatcgcc gtcaagcacg aggtggtgaa cgtgggcagc accgcagttt cgccgcagct
     4861 gtatctgcaa ctggtgcgcg atggcaacaa gccgccagga gagtcttcgt tctactccac
     4921 cttcacaggc cctgcggtgt acaccgatgc caagaagtac cagaagatcg atttcaagga
     4981 catcaagaac aaaaagttcg atattccctc agagtcgacg aacggctaca tcgcgatggt
     5041 gcagcactat ttcgccaccg catggctgct ccccgatggt gtaaagcgag agttgcggtt
     5101 ggattcagca gacatcggcg ctaacacccc tgactgctgc taccgagttc agatgattac
     5161 ccctttgggg gagttggctc ccggtcaatc taagaccatc gacgcgcgcc tgttcgccgg
     5221 tccgcaggtc gagaccatga tggaaaagtt gtaccccggc ctggaactgg tcaaggacta
     5281 cggctggctg accatcctgt ccaa
//
LOCUS       DAOMTQ010000187         5226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_3988_length_5226_cov_29.157997, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000187 DAOMTQ010000000
VERSION     DAOMTQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_5226_cov_29.157997"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="PK518_13975"
     CDS             complement(<1..2261)
                     /locus_tag="PK518_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HRM50064.1"
                     /translation="MDIQPTSAADRRLAQLARQRFVEGLCAALPDLDKKLYAFLTDLM
                     AQTATQRDMQLRRDAWMNYQAHRKAWVEDVVRAWREALVPQQSPSATMPLDGGLELVG
                     DDVIENRLLASRMALTVTEHVGASFDVARQRTQHLDGRELSSRDILRPETLCLRLVES
                     WLDAGLKREELQLALDPVQTALSELAQAQYQALLGLYDQQGVITAEELRTRQVRVASV
                     GASRSGALGRDSAGVALSRDTPFPATMLATPGGMQPPQGMPVPMAGVRPGMGGFSTPL
                     AYARQRAQGVMMQLRRMLAPSATGQGAAPAQPASSALMHALADERVEADLYYSGMATL
                     VEDYSPQAVVRVAHVVRERADEFKKKASTASEKAVIEVVALMFQSILSEDRIPPAVRV
                     WFARLQVPVLRVALAEPGFFSDLAHPARQLIDRMGSCVLGFDASAINGGALEAEIRRI
                     VQVIEQYPETGKRVFELVLAEFEKFLAKYLTEGQGTARIISVAQQVEQRETLAIQYTI
                     ELRNMLKDMPVREEIRDFLFKSWAEVLAMSAVRNGAQHAETTAFKRAAADLVWAASAK
                     PNRADRARVIAQLPALLQRLRLGLVLVGVTGAPQDAQVKLLTDTLAEAFLSKTASIPQ
                     EQIEAMTRRLENLEDFIDDETLGDIPLSAENIELLLGMDASSIHVVADDGAAVQEDMV
                     AWAQALPLGAWFTIEHNGASARVQYVWHSQRKQLHLFAALGGSSYLLQLKPLAGYMQA
                     GLLLAQEEESLTLR"
     gene            complement(2353..3306)
                     /gene="secF"
                     /locus_tag="PK518_13980"
     CDS             complement(2353..3306)
                     /gene="secF"
                     /locus_tag="PK518_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRM50065.1"
                     /translation="MEFFRIKKDIPFMKYALVLNAVSFITFALAVFFLVTRGLHLSVE
                     FTGGTVMEVAYSQTADIGKVRDKVAALGYPDVVVQNFGTSRDVMIRLPVQKGVTSAQQ
                     SEQVLAALKSEDAGVTLRRTEFVGPQVGEELVHGGLMALGMVVLGIVIYLAFRFEWKF
                     GVAAIIANLHDVVIILGFFAFFQWEFSLSVLAGVLAVLGYSVNESVVIFDRIREAFRR
                     FRKLSTHEVIDHAITSTMSRTIITHASTEAMVLSMFFFGGPSLHYFALALTIGILFGI
                     YSSVFVAAAIAMWLGVKREDLVKTSARKDGDPNDPNSGAVV"
     gene            complement(3321..5222)
                     /gene="secD"
                     /locus_tag="PK518_13985"
     CDS             complement(3321..5222)
                     /gene="secD"
                     /locus_tag="PK518_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRM50066.1"
                     /translation="MNRYPVWKYAILVIVLLIGALYTLPNFFGEAPAVQVSSAKATIK
                     VDAALQRRVEEALQAAGVSPDSVSFDGNSVRARFDTPDNQLKAKDAIQKALVSDPSDP
                     PYIVALNLVSRSPDWLTAINARPMYLGLDLRGGVHFMLQVDMQAALTKKAESYAGDLR
                     TTLREKSIRHGGISRDGQAIDIRVRDEATLTATRNLIADQFPDLLTTSSPDGEGFQLR
                     ATIKPEALRKVQEQALKQNMVTLHNRINELGVAEPVIQQQGLDRIVVQLPGVQDTAKA
                     KDILGRTATLEVRMVDEGTEARAAEIGSGPVPFGDEKYLDRNGQAVIVKKQVILTGEN
                     LTDAQPGFDGQTQEPTVNLTLDAKGSRIFKDITRENIGKRMAIVLFEKGKGEVVTAPV
                     IRSEIGGGRVQISGRMTTQEANDTALLLRAGSLAAPMEIIEEYTIGPSLGADNIDRGI
                     HSVVWGMVAIAAFMCVYYMLFGVFSTIALGMNVLLLIAVLSMLQATLTLPGIAAMALA
                     LGVAIDSNVLINERIREELRAGIAPQAAIAAGYERAWATILDSNVTTLIAGLALLAFG
                     TGPVRGFAVVHCIGILTSMFSAVFFSRGLVNLWYGRQKKLKSVAIGQVWKPEGEDNRS
                     VAGRSGSTD"
BASE COUNT          870 a         1779 c         1695 g          882 t
ORIGIN      
        1 cgcagggtca ggctttcttc ttcctgggcc agcagcagcc cggcctgcat atagccagcc
       61 aggggcttga gctgcaacag atagctgctg ccaccaaggg cggcgaacag gtgcagctgc
      121 ttgcgctggc tgtgccacac atattgcacc cgcgccgagg ctccgttgtg ctctatcgtg
      181 aaccaggcgc ccagcggcag cgcctgcgcc caggccacca tgtcctcctg cacggccgcg
      241 ccgtcgtcgg caaccacatg gatagacgag gcatccatgc ccagcagcag ttcgatgttt
      301 tccgcgctca acggtatgtc gcccagggtc tcgtcgtcga tgaagtcttc caggttttcc
      361 aggcgcctgg tcatggcctc gatctgctcc tgcgggatgg aggccgtctt cgacagaaag
      421 gcctcggcca gggtgtcggt gagcagcttg acctgtgcat cctgcggcgc gccggtgaca
      481 cccaccagaa ccagccccag acgcaaccgc tgcagcagtg ccggcaactg cgcgatgacg
      541 cgtgcgcggt cggcacggtt gggcttggcg ctggcggccc agaccaggtc ggcagcggca
      601 cgcttgaagg cggtggtttc ggcatgctgg gcgccgttgc gcaccgccga catggccagc
      661 acctcggccc aggacttgaa caggaaatca cgaatttcct cgcgcaccgg catgtccttg
      721 agcatgttgc gcagctctat ggtgtattgg atcgccagcg tctcgcgctg ctcgacctgc
      781 tgggcaacgc tgatgatgcg cgccgtgccc tggccttcgg tcaggtactt ggcaaggaac
      841 ttctcgaact ccgccaacac cagctcgaac acgcgcttgc cagtctcggg gtattgctcg
      901 atcacctgca cgatgcggcg gatttcggcc tccagcgcgc caccgttgat ggcgctggcg
      961 tcaaagccca gcacgcagga gcccatgcgg tcgatgagct ggcgcgccgg atgggccagg
     1021 tcgctgaaga accccggctc ggccagcgcc acgcgcagca ccggcacctg caggcgtgca
     1081 aaccacacgc gcaccgcggg cggtatgcgg tcttcgctga ggatgctctg gaacatcagg
     1141 gccaccacct cgatgacggc cttctcgctg gccgtgctgg cctttttctt gaattcgtcc
     1201 gcgcgctcgc gcaccacatg ggccacgcgc accacggcct gggggctgta gtcctcgacc
     1261 agcgtggcca tgccgctgta gtacaggtcg gcctccacgc gctcgtcggc caatgcatgc
     1321 atcagggccg acgaggccgg ctgcgccggg gccgcgccct ggcccgtggc cgacggcgcc
     1381 agcatgcgcc gcagctgcat catcaccccc tgcgcacgct ggcgcgcata ggccaggggg
     1441 gtggaaaaac cgcccatccc cgggcgtacg ccagccatgg gcaccggcat gccctggggt
     1501 ggctgcatgc cgccgggcgt tgccagcatg gtggcgggaa acggtgtatc ccggctcagg
     1561 gccacaccgg cgctgtcgcg gcccaaggcg cccgagcggc tcgcccccac gctcgccacc
     1621 cgcacctggc gcgtgcgcag ctcctcggcc gtgatcacac cctgctggtc atacagcccg
     1681 agcaacgcct ggtactgcgc ctgggccagc tcggacaggg cggtctgcac cggatcgagc
     1741 gccagctgca gctcctcgcg cttgaggccg gcatccagcc acgactccac cagccgcagg
     1801 cacagggtct cggggcgcag gatgtcgcgg ctggaaagct cacgtccgtc caggtgctgg
     1861 gtacgctggc gcgcgacgtc aaaggacgcg cccacatgct cggtgacggt cagcgccatg
     1921 cgcgaggcca gcagccggtt ctcgatcacg tcgtcgccaa ccagctccag tcccccgtcc
     1981 aggggcatgg tggcggaggg cgactgctgc ggcaccagag cctcgcgcca ggcgcgcacc
     2041 acatcctcca cccaggcctt gcggtgggcc tggtagttca tccaggcgtc gcggcgcagc
     2101 tgcatgtcgc gctgcgtcgc cgtctgcgcc atcagatcgg tcaggaacgc atacagcttc
     2161 ttgtccagat ccggcaaggc cgcgcacagg ccttccacaa aacgctgccg cgccagctgg
     2221 gccaggcggc ggtcggcggc agaggtgggc tggatgtcca tagaccttgg atcttagccg
     2281 ttgccctcgc cctgttgtgg cgtatccaag acagtttcag tgcgttgcca cagcgccggc
     2341 gcgagcgccc gcttagacca ccgccccgga gttggggtcg ttgggatcgc catccttgcg
     2401 cgccgaggtc ttcaccagat cctcgcgctt gacacccagc cacatggcga tcgccgccgc
     2461 cacgaacacc gaggaataga tgccgaacag aatgcctatg gtcagcgcca gcgcaaagta
     2521 gtgcaggctg gggccgccga agaagaacat cgacagcacc atggcctcgg tcgaggcgtg
     2581 ggtgatgatg gtgcggctca tggtcgaggt gatggcgtgg tctatgacct cgtgcgtgga
     2641 gagcttgcga aagcgccgga aggcctcgcg aatccggtca aagatcacca ccgactcgtt
     2701 caccgaatag cccagcacgg ccagcacccc ggccagcacc gagagcgaga actcccactg
     2761 gaagaaggcg aagaagccca ggatgatgac cacgtcgtgc aggttggcga tgatggccgc
     2821 gacgccgaac ttccactcga aacggaacgc caggtagatg acgatgccca gcaccaccat
     2881 gcccagcgcc atcaggccgc cgtgcaccag ctcctcgccc acctgcgggc cgacgaactc
     2941 cgtgcgccgc agcgtcacgc ccgcgtcctc gctcttcagc gccgccagca cctgctcgct
     3001 ttgctgggcc gaggtcacgc ccttttgcac cggcaggcgg atcatcacgt cgcgggacgt
     3061 cccgaagttc tgcaccacca cgtcggggta gcccagggcc gccaccttgt cgcgcacctt
     3121 gccgatgtcg gccgtctgcg agtaggccac ctccatgacg gtgccgccgg tgaattccac
     3181 cgacagatgc aggccacgcg tgaccagaaa gaacaccgcc agggcgaagg tgatgaagga
     3241 caccgcgttg agcaccaacg cgtacttcat gaacggaatg tcttttttga tgcggaaaaa
     3301 ctccatggtc tttcctcctt ttaatcagtg ctgccgctgc ggccggccac ggagcggttg
     3361 tcttcgccct cgggcttcca gacttggccg atggccacgc tcttgagttt cttctggcgt
     3421 ccataccaga gattcaccag gccgcgcgag aagaacacgg ccgagaacat gctggtcaga
     3481 atgcctatgc aatgcaccac ggcaaaaccg cgcaccggcc cagtgccaaa ggccagcagc
     3541 gccagaccgg cgatcagcgt ggtcacgttg gagtccagga tggtggccca ggcgcgctcg
     3601 tagcccgcgg caatcgccgc ctgcggcgcg atgccggcgc gcagctcctc gcggatgcgc
     3661 tcgttgatca gcacgttgga gtcgatggcc acgcccagcg ccagcgccat ggcggcaatg
     3721 ccgggcaggg tcagcgtggc ctgcagcatg gacagcacgg cgatcagcag cagcacgttc
     3781 atgcccagcg caatcgtgga gaacacgccg aacagcatgt agtacacgca catgaaggcg
     3841 gcaatggcca ccatgcccca gaccacggaa tggatgccgc gatcgatgtt gtcggcgccc
     3901 aggctggggc caatggtgta ttcctcgatg atttccatgg gcgcggcgag cgagccggcg
     3961 cgcagcagca gggccgtgtc gttggcttcc tgcgtcgtca tgcggcccga gatctgcacc
     4021 cggccaccgc cgatttcgct gcgaatcacc ggcgccgtga cgacctcgcc cttgcccttc
     4081 tcgaacagca cgatggccat gcgcttgccg atgttctcgc gcgtgatgtc cttgaagatg
     4141 cgcgagccct tggcgtccag cgtcaggttg acggtgggct cctgggtctg gccgtcaaag
     4201 ccgggctggg cgtcggtcag gttttcgccg gtgaggatga cctgcttctt cacgatcacg
     4261 gcctggccgt tgcgatccag gtatttctcg tcgccaaagg gcacggggcc gctgccgatc
     4321 tcggcggcgc gcgcctcggt gccctcgtcc accatgcgca cctccagggt ggcggtacgg
     4381 cccaggatgt ccttggcttt ggccgtgtcc tgcacgcccg gcagctgcac cacgatgcgg
     4441 tccaacccct gctgctggat caccggctcg gccacgccca gctcgttgat acggttgtgc
     4501 agcgtgacca tgttctgctt gagcgcctgc tcctgcacct tgcgcagggc ctcgggcttg
     4561 atggtggcgc gcagctggaa gccctcgcca tccgggctgc tggtggtcag caggtcgggg
     4621 aactggtctg cgatcaggtt gcgcgtggcc gtcagcgtgg cctcgtcgcg cacgcggatg
     4681 tctatggcct ggccgtcacg gctgatgccg ccgtggcgga tgctcttctc gcgcagcgtg
     4741 gtgcgcagat cgcccgcgta agactcggcc ttcttggtga gcgctgcctg catgtccacc
     4801 tgcagcatga agtgcacgcc gccgcgaagg tccaggccca ggtacatggg gcgggcattg
     4861 atggcggtga gccagtcggg cgagcgcgac accaggttca gcgccacgat gtagggcggg
     4921 tcggacgggt cgctgaccag ggccttctgg atcgcgtcct tggccttgag ctggttgtcc
     4981 ggcgtgtcaa agcgcgcgcg caccgagttg ccgtcgaagc tcaccgagtc cgggctcacg
     5041 ccggccgcct gcagcgcctc ttcgacgcgc cgctgcagcg cggcatcgac cttgatggtg
     5101 gccttggccg atgacacctg caccgcaggc gcctcgccaa agaaattggg cagggtgtac
     5161 aaggccccta tcagcagcac gatcaccagg atcgcgtact tccagaccgg ataacggttc
     5221 atgatc
//
LOCUS       DAOMTQ010000188         5117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4133_length_5117_cov_26.565784, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000188 DAOMTQ010000000
VERSION     DAOMTQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_5117_cov_26.565784"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3969
                     /locus_tag="PK518_13990"
     CDS             <1..3969
                     /locus_tag="PK518_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRM50067.1"
                     /translation="YRYVAHNGEINTVRGNYNWMLAREGVMASPVLGEDLKKLYPISF
                     AGQSDTATFDNCLELLTMAGYPISQAVMMMIPEPWEQHEGMDERRRAFYEYHAAMIEP
                     WDGPASIVFTDGRQIGATLDRNGLRPSRYVVTEDDLVILASEAGVLPVPDGKVLRKWR
                     LQPGKMLLIDLEQGRLIEDDELKANIVNTKPYKQWIENLRVKLDEVQAPPAAPVASEL
                     PLLERQQAFGFTQEDIKFLLAPMAANGEEGIGSMGNDSPLAVLSERSKPLYNYFRQMF
                     AQVTNPPIDPIREAIVMSLVSFVGPKPNLLDINQVNPPMRLELHQPVLDFEGMAKLRN
                     IEAHTHGKFKSATIDITYPLSWGKQGVEAKLASLCAQAVDEIKGGANILIISDRLLSA
                     NQVAIPALLALSAIHQHLVREGLRTTTGLVVETGTAREVHHFAVLAGYGAEAVHPYLA
                     LETLVEMHQELPGALSADKAIYNYVKAIGKGLSKIMSKMGVSTYMSYCGAQLFECVGL
                     NSDTVAKYFTGTASRVEGIGVFEIAEESIRTHIAAFGNDPVLATMLETGGEYAWRTRG
                     EEHMWTPDAIAKLQHATRGGNFSTYKEYAQIINDQSKRQLTLRGLFEFKFDPAQAVPL
                     DEVEPAKEIVKRFATGAMSLGSISTEAHATLAVAMNRIGGKSNTGEGGEDPARYRKEL
                     KGISITKGEKLSTIIGADKVESDIELQDGDSLRSKIKQVASGRFGVTAEYLSSSDQIQ
                     IKMAQGAKPGEGGQLPGGKVSEYIGQLRHSVPGVGLISPPPHHDIYSIEDLAQLIHDL
                     KNVAPHADISVKLVSEVGVGTIAAGVAKCKSDHVVIAGHDGGTGASPWSSIKHAGSPW
                     EIGLAETQQTLVLNRLRGRIRVQADGQMKTGRDVVIGALLGADEFGFATAPLVVEGCV
                     MMRKCHLNTCPVGVATQDPQLRAKFSGKPEHVVNYFFFVAEEARQIMAQLGVRKFDDL
                     IGHTELLDMRRGLQHWKAQGLDFSRLFAQPQVPADVARFHVETQDHCLEKALDVKLIE
                     RCQPAIQKGEPVRLMEVARNVNRSVGAMLSGAVTKAHPEGLPDDSIRIHFEGTGGQSF
                     GAFLCKGITLNLMGEANDYTGKGLSGGRIVVRPSHEFRGEAARNTIVGNTVMFGATSG
                     QAFFAGVAGERFGVRLSGATAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGVA
                     YVYDEDGRFDGRCNLAMVSLERVLPADEQVASMDPGHWHKGQTDEEQLKKLLEEHSRQ
                     TGSRRARELLDNWAAARSRFVKVFPTEYKRALVEMYERKVLEEQATPAQGAPKTEVIA
                     AK"
     gene            4122..>5117
                     /locus_tag="PK518_13995"
     CDS             4122..>5117
                     /locus_tag="PK518_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRM50068.1"
                     /translation="MGKTTGFMEYERIEEGYPAPAERVKHYKEFVIGLDQSQAKTQAA
                     RCMDCGIPFCNHGCPVNNIIPDFNDLVYHGDWKSASTVLHSTNSFPEFTGRICPAPCE
                     AACTLNFNSDAVGIKSIEHAIVDRAWAEGWITPQKAARKTGKKVAIVGSGPAGLAAAQ
                     QLARVGHSVTLFEKNDRVGGLLRYGIPDFKLDKGHIDQRVKQLEAEGVVIRTSVFVGA
                     EKDGLGKDSKVTNWAKETITPEQLHKDFDAVLLTGGAEQSRDLPAPGRDLAGIHFAME
                     FLPQQNKVNAGDKLKGQIRADGKHVIVIGGGDTGSDCVGTSNRHGAASVTQFEVMP"
BASE COUNT          995 a         1723 c         1651 g          748 t
ORIGIN      
        1 taccgctacg tggcgcacaa cggcgagatc aacaccgtgc gcggcaacta caactggatg
       61 ctggcgcgcg agggcgtgat ggcctcgccg gtgctgggcg aggacctgaa aaagctctac
      121 cccatcagct ttgccggcca gagcgacacc gccaccttcg acaactgcct ggaactcttg
      181 accatggccg gctacccgat cagccaggcg gtgatgatga tgattcccga gccctgggag
      241 cagcacgagg gcatggacga gcgccgccgc gccttctatg aataccacgc cgccatgatc
      301 gagccctggg acggcccggc ctccatcgtc tttaccgacg gccgccagat cggcgccacg
      361 ctggaccgca acggcctgcg gccgtcccgc tacgtggtga ccgaggacga cctggtcatc
      421 ctggcctccg aagccggcgt gctgcccgtg cctgacggca aggtgctgcg caagtggcgt
      481 ttgcagccgg gcaagatgct gctcatcgac ctggagcagg gccgcttgat cgaagacgac
      541 gagctcaagg ccaacatcgt caacaccaag ccctacaagc agtggatcga gaacctgcgc
      601 gtcaagctcg acgaggtgca ggcgccgccc gccgcgcccg tggcgtctga attgccgctg
      661 ctcgagcgcc agcaggcctt tggcttcacg caggaggaca tcaagttcct gctcgccccc
      721 atggccgcca acggcgagga gggcataggc tccatgggca acgacagccc gctggcggtg
      781 ttgtccgagc gcagcaagcc gctctacaac tacttccgcc agatgtttgc ccaggtgacc
      841 aacccgccga tcgacccgat ccgcgaggcc atcgtcatga gcctggtgtc cttcgtcggc
      901 cccaagccca atctgctgga catcaaccag gtcaaccccc ccatgcgctt ggagctgcac
      961 cagccggtgc tggacttcga gggcatggcc aagctgcgca acatcgaggc gcacacgcac
     1021 ggcaagttca agagcgcgac catcgacatc acctacccgc tgtcctgggg caagcagggc
     1081 gtggaagcaa aattagcgtc tctgtgcgcg caggcggtcg atgagatcaa gggcggggcc
     1141 aacatcctca tcatcagcga ccggctgctg tcggccaacc aagtcgccat tccggccctg
     1201 ctggccctgt ctgccatcca ccagcacctg gtgcgcgagg gcctgcgtac caccaccggc
     1261 ctggtggtcg agaccggcac ggcgcgggag gtccaccact tcgccgtgct cgccggctat
     1321 ggcgccgagg ccgtccaccc ctacctggcg ctggagaccc tggtggagat gcaccaggag
     1381 ctgcccggcg ccctgtcggc cgacaaggcc atctacaact acgtcaaggc gatcggcaag
     1441 ggcctgtcca agatcatgtc caagatgggc gtgtccacct atatgagcta ctgcggcgcg
     1501 cagctgttcg agtgcgtggg cctgaacagc gacaccgtgg ccaagtactt caccggcacc
     1561 gccagccgcg tcgagggcat aggcgtgttc gagatcgccg aggaaagcat ccgcacccac
     1621 atcgccgcct ttggcaacga cccggtgctg gccaccatgc tcgagaccgg cggcgaatac
     1681 gcctggcgca cccgtggcga agagcatatg tggacgcccg acgccatcgc caagctgcag
     1741 cacgccacgc gcggcggcaa cttcagcacc tacaaggaat acgcgcagat catcaacgac
     1801 caatccaagc gccagctcac gctgcgcggc ctgttcgagt tcaagttcga cccggcccag
     1861 gccgtgccgc tcgacgaggt cgaacccgcc aaggaaatcg tcaagcgctt tgccaccggc
     1921 gccatgtcgc tgggctcgat ttccaccgag gcgcatgcca cgctggccgt ggccatgaac
     1981 cgcatcggcg gcaagagcaa caccggcgag ggcggcgaag acccggcgcg ctatcgcaag
     2041 gaattgaagg gcatcagcat caccaagggt gagaagctct cgaccatcat cggcgccgac
     2101 aaggttgagt ccgacatcga gctgcaagac ggcgacagcc tgcgctccaa gatcaagcag
     2161 gtggcgtccg ggcgctttgg cgtgacggcc gaatatttgt cgtcgtccga ccagatccag
     2221 atcaagatgg cccagggtgc caagcccggc gagggcggcc agctgcccgg cggcaaggtg
     2281 tcggaataca tcgggcagct gcgccacagc gtgcccggcg tgggcctcat cagcccgccg
     2341 ccgcaccacg acatttattc catcgaagac ctggcgcagc tgattcacga cctgaagaac
     2401 gtcgcgccgc acgcggatat ttcggtcaag ctggtgtctg aagtgggcgt cggcacgatt
     2461 gccgcgggcg tcgccaagtg caagagcgac catgtggtca ttgccgggca cgacggcggc
     2521 acgggcgcct cgccctggtc gtccatcaag cacgccggca gcccctggga aatcggcctg
     2581 gccgagaccc agcagaccct ggtcttgaac cgcctgcgcg gccgtatccg cgtgcaggcc
     2641 gacggccaga tgaagaccgg gcgcgacgtg gtgattggcg cgctgctggg cgcggacgag
     2701 ttcggctttg ctaccgcgcc gctggtcgtc gagggctgcg tgatgatgcg caaatgccac
     2761 ctcaacacct gcccggtggg cgtggccacg caagacccgc agctgcgcgc caagttctcg
     2821 ggcaagcccg agcatgtggt gaactacttc ttcttcgttg ccgaagaggc gcgccagatc
     2881 atggcgcagc tgggcgtgcg caagtttgac gacttgatcg gccataccga gctgctcgac
     2941 atgcgccgtg gcctgcaaca ctggaaggcc cagggcctgg acttctcgcg cctgttcgcc
     3001 cagccgcagg tgccggccga cgtggcacgc tttcatgtcg agacgcaaga ccactgcctg
     3061 gaaaaggcgc tggacgtcaa gcttatcgag cgctgccagc ccgccattca gaagggcgag
     3121 ccggtgcggt tgatggaggt ggcgcgcaac gtgaaccgtt cggtaggcgc catgctctcg
     3181 ggcgcggtca ccaaggcgca ccccgagggg ttgcccgatg acagcatccg catccacttc
     3241 gagggcacgg gcgggcagag ctttggcgcc ttcctgtgca agggcatcac gctgaatctg
     3301 atgggcgagg ccaacgacta caccggcaag ggcctgtcgg gcgggcgcat cgtcgtgcgc
     3361 cccagccacg agttccgcgg cgaggcggcg cgcaacacca tcgtcggcaa caccgtgatg
     3421 tttggcgcca ccagcggcca ggccttcttt gccggcgtgg ccggcgagcg ctttggcgtg
     3481 cgcctgtcgg gcgccacggc ggtcgtcgag ggcgtgggcg accacggctg cgaatacatg
     3541 accggcggca ccgtggtggt gctgggcaag accgggcgca acttcgccgc cggcatgagc
     3601 ggcggcgtag cctatgtcta cgacgaggac gggcgctttg acggccgctg caacctggcc
     3661 atggtgagcc tggagcgcgt gctgcccgcc gacgagcagg tcgccagcat ggaccccggc
     3721 cactggcaca agggccagac cgacgaggag cagctgaaga agctgttaga ggagcacagc
     3781 cgccagaccg gcagccgccg cgcgcgcgaa ctgctggaca actgggccgc cgcgcgcagc
     3841 cgcttcgtca aggtcttccc caccgagtac aagcgtgccc tggttgagat gtatgagcga
     3901 aaagtgcttg aagagcaggc tacgcctgcg caaggagctc ctaaaacaga agtaatagcg
     3961 gctaagtaga ccggacgcaa ctccctctcc cgctggcggg agagggcagg ggtgagggtg
     4021 gcttccccag cgggcacccc cacccccacc cttccccgct acgcagggga gggagttcaa
     4081 ccccaagaac acccacagac acagcgaaag acaccacaat catgggcaag accaccggtt
     4141 tcatggaata cgagcgcatc gaggagggct acccggcccc ggccgagcgc gtcaagcact
     4201 acaaggagtt cgtcatcggc ctggatcaat cccaggccaa gacccaggct gcgcgctgca
     4261 tggactgcgg cataccgttc tgcaaccacg gctgcccggt gaacaacatc attccggact
     4321 tcaacgacct cgtctatcac ggcgactgga agagcgccag cacggtgctg cacagcacca
     4381 acagcttccc cgagttcacc ggccgcatct gccccgcgcc ctgcgaggcc gcctgcacgc
     4441 tcaacttcaa cagcgatgct gtcggcatca agagcatcga gcacgccatc gtcgaccggg
     4501 catgggccga gggctggatc acgccgcaga aggcagcgcg caagaccggc aagaaggtgg
     4561 ccatcgtcgg cagcggcccg gccggcctgg cggccgccca gcagctcgcc cgcgtgggcc
     4621 acagcgtgac gctgttcgag aagaacgacc gcgttggcgg cctgctgcgc tacggcatcc
     4681 ccgacttcaa gcttgacaag ggccatatcg accagcgcgt gaaacagctc gaagccgagg
     4741 gcgtggtgat ccgcaccagc gtctttgtcg gcgccgagaa ggacggcctg ggcaaagaca
     4801 gcaaggtcac caactgggcc aaggaaacca tcacccccga gcagctgcac aaggactttg
     4861 acgccgtgct gctgaccggc ggtgccgagc aaagccgcga cctgcccgcg ccgggccgtg
     4921 atctggccgg catccacttc gccatggagt ttttgccgca gcaaaacaag gtcaacgcgg
     4981 gcgacaagct caagggccag atccgtgccg atggcaagca tgtgatcgtg attggcggcg
     5041 gcgacaccgg cagcgactgc gtgggcacca gcaaccgcca cggcgcggcc agcgtgacgc
     5101 agttcgaggt catgccc
//
LOCUS       DAOMTQ010000189         4853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4496_length_4853_cov_23.960192, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000189 DAOMTQ010000000
VERSION     DAOMTQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4496_length_4853_cov_23.960192"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PK518_14000"
     CDS             complement(<1..794)
                     /locus_tag="PK518_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM50069.1"
                     /translation="MAAAPANYADFHRRSIDERDAFWAEQAQLIEWQQAPQQICNYDK
                     PPFAKWFVGGTTNLCHNAVDRHLAARAQQNALVAISTETDTERAYTFAELHAEVQRMA
                     AVLQSLGVKQGDRVLIYMPMIAEAAFAMLACVRIGAIHSVVFGGFASSSLASRIEDAE
                     PTVIVSADAGSRGGKGVPYKPLLDEAIDLSAHKPAAVLLVDRGLAPAAMRAGRDHDWA
                     ALREQHMDAQVPCVWLESTHPSYTLYTSGTTGKPKGVQRDTGGYTVA"
     gene            complement(995..1606)
                     /locus_tag="PK518_14005"
     CDS             complement(995..1606)
                     /locus_tag="PK518_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRM50070.1"
                     /translation="MLLDATESQLVLVDYQERLMPAIFEGQAALANAIRLAQIARLVD
                     VPVWGTEQNPTRLGSNDAQLRALCQRTLSKMHFSAAADGLGAWLRPPAKPQGGNARSL
                     PKHLQKAREQEQQAIERPSIVIAGCEAHVCLLQTALELLEEEFEVWVVTDACSSRTER
                     NRDAAFDRLAGAGAELVTTEMVAFEWLRSCEHPAFKDMLAFVK"
     gene            complement(1664..2563)
                     /locus_tag="PK518_14010"
     CDS             complement(1664..2563)
                     /locus_tag="PK518_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM50071.1"
                     /translation="MDWDNLRYFLELARTGTLAAAARRTGVEHTTVARRIQALEKQMG
                     APLFAREATGHRLTEAGRQLLPAVEAMEAAVLGMERAAPANAASGGPSGLVRVGVSEG
                     FGTLILAPQLARLTQRHPHLSIDLLALPRMLHLSRREADIVISLERPKRGSVIVTKLA
                     DYRLYLYGQREYLARRPLVARREDLRHHDFISYVDDLLFSKELQFIDQLWPPERFALR
                     STSITAQYEAVRAGAGLAVLPAFLADRDPILARVLPHEAVFTRTFWMSMPAEGKHLAR
                     MQAVWGFLKEVGAKEGARLVPAA"
     gene            2687..4204
                     /locus_tag="PK518_14015"
     CDS             2687..4204
                     /locus_tag="PK518_14015"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRM50072.1"
                     /translation="MDASTAVQAPTVKLLINGQLVESKTTQWRNVVNPATQEVLARVP
                     FATPDEVNAAVANAKEAFKTWRKTPIGTRARIFLKLQQLIRENMKELAALLTAEQGKT
                     LPDAEGDVFRGLEVVEHAAAIGNLQLGELANNVASGVDTYTVMQPLGVCAGITPFNFP
                     AMIPLWMFPMAIATGNTFVLKPSEQDPMVTMRLCELALEAGIPPGVLNVVHGGEDVVN
                     AICDHPDIKAISFVGSTKVGTHVYNRASLAGKRVQCMMGAKNHAIVLPDANKEQSINA
                     IVGASFGAAGQRCMAISVVLLVGEAQKWIPDFVAKAKALKISAGSTPGTDVGPVISCA
                     ARERVEGLIERGIADGAVIELDGRKPNVPGFEKGNFVGPTIFSGVKPGMSVYDQEIFG
                     PVLCIAGADDLEQAIEFINANPNGNGTAIFTQNGAAARMFQEDIDVGQVGINLPVPVP
                     VPLFSFTGSRASKLGDLGPYGKQVVLFYTQTKTVTARWFDDSTLHHGVNTTISLK"
     gene            4201..4593
                     /locus_tag="PK518_14020"
     CDS             4201..4593
                     /locus_tag="PK518_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HRM50073.1"
                     /translation="MTGPRAAALALALCLVGQAAWAQAGGACRPGGTVEETNACAVRD
                     YQEADTAIQVLYGDVMRALSAHERPALRQDHANWQRARITRCKEAQRTNEALPEWPRL
                     YHECLTAQTQARRQALSHWLHHGQAPQP"
     gene            4618..>4853
                     /locus_tag="PK518_14025"
     CDS             4618..>4853
                     /locus_tag="PK518_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM50074.1"
                     /translation="MNFELTEDQRAFADTARAFAEGQLAPHAAHWDAEGIFPVDAIAK
                     AGELGFCGLYAPENAGGLALPRLDATLVFEELAAV"
BASE COUNT          809 a         1661 c         1621 g          762 t
ORIGIN      
        1 gccacggtgt agccgccggt gtcgcgctgc acgcccttgg gcttgccggt ggtgccgctg
       61 gtgtacaggg tgtagctggg gtgggtggat tccagccaca cgcagggcac ctgcgcgtcc
      121 atatgctgct cgcgcagcgc cgcccagtcg tggtcgcgcc cggcgcgcat ggcagccggg
      181 gccaggccgc ggtccaccag cagcacggcg gcgggcttgt gcgccgacag gtcgatggcc
      241 tcgtccagca gcggcttgta gggcacgccc ttgccgccgc gcgagccggc atcggcactg
      301 acgatgacag tgggctcggc atcctcgatg cgcgatgcca gcgaggacga ggcaaagccc
      361 ccaaacacta ccgaatggat ggcgccaatg cgcacgcagg ccagcatggc aaaggcggcc
      421 tcggcaatca tgggcatgta gatcagcacg cggtcgccct gcttgacgcc cagcgactgc
      481 agcacggcgg ccatgcgctg cacctcggcg tgcaattcgg cgaaggtgta ggcgcgctcg
      541 gtatccgtct cggtggaaat ggccaccagc gcgttttgct gcgcgcgcgc cgccaggtga
      601 cgatcgacag cgttgtggca caggttggtg gtaccgccca cgaaccactt ggcaaacggc
      661 ggcttgtcgt agttgcagat ctgctgcggc gcttgctgcc attcgatcag ctgggcctgc
      721 tcggcccaga aggcgtcgcg ctcgtcaatc gagcggcggt ggaaatcggc gtagttggct
      781 ggggctgcgg ccatggtgct gtctcctggg agtggcatgc gccgtggtgg cgcgggcgat
      841 gggttatcca aaattcataa ttattcacat ggcgcttgcg ggaagctgac gtgccagatg
      901 ggcattggcg cgcgctgaaa acacacctgc aggcgtgacc ctgaccccat ccgaccttcc
      961 cccggctagt gtcctgaaat ggcgctaccc aggtttactt gacgaacgcc agcatgtcct
     1021 tgaacgccgg atgctcgcag ctgcgcagcc attcgaaggc caccatctcg gtggtcacca
     1081 gctcggcgcc ggcgccggcc aggcggtcga atgcggcgtc gcggtttcgt tcggtgcgcg
     1141 agctgcaggc gtctgtcacg acccagactt caaactcttc ctccagcagc tccagcgccg
     1201 tctgcagcag gcagacatgg gcctcgcagc cggcgatgac gatgctgggg cgctcgatgg
     1261 cctgctgttc ctgctcgcgc gccttttgca ggtgcttggg caggctgcgc gcattgccgc
     1321 cctggggttt ggccggcggg cgcagccagg cgcccaggcc gtcggccgcg gcgctgaagt
     1381 gcatcttgga cagggtgcgc tggcacaggg cgcgcagctg cgcgtcgttg cttcccaagc
     1441 gcgtggggtt ttgctcggtg ccccagacgg gcacgtccac caggcgggcg atttgcgcca
     1501 gcctgatggc gttggccagc gccgcctggc cttcaaaaat ggcgggcatc aggcgttcct
     1561 ggtagtccac gagcacgagt tgggattcgg tggcgtcaag cagcatggcg gtcacggggt
     1621 cagaaacaaa gatgcggtca ttgtcgcagg cgggcggcgg gggctatgcg gccggcacca
     1681 gccgcgcgcc ctctttcgcc cccacctctt tcagaaagcc ccacaccgcc tgcatgcgcg
     1741 ccaggtgctt gccctcggcc ggcatgctca tccagaaggt gcgggtgaat accgcctcgt
     1801 gcggcagcac gcgcgccagg atggggtcgc ggtcggccag aaaggccggc agcacggcca
     1861 ggccggcgcc ggcgcgcacg gcctcgtact gggcggtgat gctggttgag cgcagggcaa
     1921 agcgctcggg cggccagagc tggtcgatga actgcagttc tttgctgaac aacagatcgt
     1981 ccacatagct gatgaaatcg tggtggcgca ggtcttcgcg ccgcgccacc agcgggcggc
     2041 gcgccaggta ttcgcgctgg ccgtacaggt aaagccggta gtcggctaat tttgtgacga
     2101 tgaccgatcc gcgcttgggc cgctccagcg agatgacgat gtcggcctca cgccggctca
     2161 ggtgcagcat gcgcggcagc gccagcaggt ctatggacag gtgcgggtgg cgctgtgtca
     2221 ggcgcgccag ctgcggcgcc aggatcagcg tgccaaagcc ctcggacaca ccgacgcgca
     2281 ccaggcccga cgggccgccg ctggccgcgt tggccggcgc ggcgcgctcc atgcccagca
     2341 cggcggcctc catggcttcc accgcgggca gcagctggcg gccggcctcg gtcaggcggt
     2401 ggccggtggc ctcgcgcgcg aacagcggcg cgcccatctg cttttccagc gcctggatgc
     2461 gccgcgccac cgtggtgtgc tccacgcccg tgcgccgcgc ggccgcggcc agggtgccgg
     2521 tacgggccag ctcaagaaag tagcgcaggt tgtcccagtc catggtggtc gattgcgatg
     2581 tgcaaatttg caaagctgaa gtgcgcaatt atctattgca tgggtaattt cgcacacgta
     2641 ccatggcacc cattcatcca ccacccctac ccaggagacc agcgccatgg acgcatccac
     2701 cgccgtacaa gcccccaccg tcaagctgct gatcaacggc cagctggtcg aatccaagac
     2761 cacccagtgg cgcaacgtgg tcaaccccgc cacgcaagag gtgctggcgc gcgtgccctt
     2821 tgccacaccc gacgaggtga acgccgccgt ggccaacgcc aaggaagcct tcaagacctg
     2881 gcgcaagacc cccatcggta cccgcgcgcg catcttcctc aagctgcagc agctgatccg
     2941 cgaaaacatg aaggagctgg ccgcgctgct gacggccgaa cagggcaaga ctctgcccga
     3001 cgccgagggc gatgtgttcc gcggcctgga ggtggtcgag catgccgccg ccattggcaa
     3061 cctgcagctc ggtgagctgg ccaacaacgt ggccagcggc gtggacacct acaccgtgat
     3121 gcagccgctg ggcgtgtgcg ccggcatcac gccgttcaac ttccccgcca tgatcccgct
     3181 gtggatgttc cccatggcga ttgccacggg taataccttc gtgctcaagc cctccgagca
     3241 ggacccgatg gtcaccatgc gcctgtgcga gctggcgctg gaggccggca ttccccccgg
     3301 cgtgctgaac gtggtgcacg gcggcgagga cgtggtcaac gccatctgcg accacccgga
     3361 catcaaggcg atcagcttcg tcggctcgac caaggtcggc acgcacgtct acaaccgcgc
     3421 cagcctggcc ggcaagcgcg tgcagtgcat gatgggtgca aagaaccacg ccatcgtcct
     3481 gcccgacgcg aacaaggagc agtccatcaa cgccatcgtc ggcgcatcgt tcggcgcggc
     3541 cggccagcgc tgcatggcga tctcggtggt gctgctggtg ggcgaggcgc agaagtggat
     3601 tcccgacttc gtcgccaagg ccaaggcgct caagatttcg gccggcagca cgcccggcac
     3661 cgacgtgggc ccggtcatct cctgcgccgc ccgcgagcgc gtcgagggcc tgatcgagcg
     3721 cggcattgcc gacggcgccg tcatcgagct ggacggccgc aagcccaacg tgcccggctt
     3781 tgagaagggc aacttcgtcg gcccgacgat cttcagcggc gtcaagcccg gcatgagcgt
     3841 ctatgaccag gaaatcttcg gccccgtgct gtgcatcgcc ggcgccgacg acctggagca
     3901 ggccatcgag ttcatcaacg ccaaccccaa cggcaacggc acggccatct tcacgcagaa
     3961 cggcgcggcc gcgcgcatgt tccaggaaga catcgacgtc ggccaggtcg gcatcaacct
     4021 gcccgtgccg gtgccggtcc cgctgttctc gttcaccggc agccgcgcct ccaagctcgg
     4081 tgacctgggc ccctacggca agcaggtggt gctgttctac acgcagacca agacggtgac
     4141 ggcccgctgg tttgacgaca gcacgctgca ccatggcgtg aacaccacca tcagcctgaa
     4201 gtgaccggcc cgcgcgctgc cgcccttgcc cttgcgctgt gccttgtggg ccaggccgcc
     4261 tgggcgcagg ccggcggcgc ctgccgcccc ggcggcaccg ttgaggaaac caacgcctgc
     4321 gccgtgcgcg actaccagga ggccgacacg gccatccagg tgctgtacgg cgatgtgatg
     4381 cgcgccctgt cggcgcatga gcgccctgcc ctgcgccagg atcacgccaa ctggcagcgc
     4441 gcccgcatca cccgatgcaa agaagcgcag cgcaccaacg aagcacttcc cgaatggccg
     4501 cgcctgtacc acgaatgcct gacggcacag acccaggcgc gccgccaggc cttgagccac
     4561 tggctgcacc acggccaggc gccgcaacca taaaaccaac gaccaggagg ccccgccatg
     4621 aactttgaac tgaccgaaga ccagcgcgcc tttgccgaca ccgcgcgtgc gtttgccgaa
     4681 ggccaactgg cgccgcacgc cgcgcattgg gacgccgagg gcatcttccc ggtcgacgcc
     4741 attgccaagg ccggcgagct gggcttttgc ggcctgtacg cgcccgaaaa cgctggcggc
     4801 ctggctctgc cgcgcctgga cgccaccctg gtcttcgaag agctggccgc cgt
//
LOCUS       DAOMTQ010000190         4829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4531_length_4829_cov_21.985756, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000190 DAOMTQ010000000
VERSION     DAOMTQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4531_length_4829_cov_21.985756"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /gene="mltB"
                     /locus_tag="PK518_14030"
     CDS             complement(<1..1032)
                     /gene="mltB"
                     /locus_tag="PK518_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HRM50075.1"
                     /translation="MTFLTFKTTFLIAACALSISATAKNNPKKQTAKAAPAPHYAESA
                     EAMRFADDLAERRGLDREWVRQAIGQARLLREVPRLVLPPPKGQPKNWAAYRARFVEP
                     VRIRAGLRFWQDNAETLARAEKEYGVPAEIIVGIIGVETIYGQHMGGYRVIDALATLA
                     FDFPDAHPRAPERRAFFQRELEQFLSLMDRTGTDPHSPRGSYAGAMGLGQFMPSSWSR
                     WAVDFDGDGRIDLWRSAPDAIGSVANYFVAHGWHSGMATHYAVAFDTASLQLDELLAP
                     DILPTFSVASMAAKGAQVQGDGATYAGPLALVELQNGAAPASYVAGTENFYAVTRYNW
                     SSYYAMAVIE"
     gene            complement(1029..3089)
                     /locus_tag="PK518_14035"
     CDS             complement(1029..3089)
                     /locus_tag="PK518_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRM50076.1"
                     /translation="MTLAGRLAPLSRLPRDARDTLFLLAVIACCIAPLAAHLPAWASA
                     MTATLLAWRGWLAVGGRPLPGRWLTALLLALVVLLTLLSHGTIVGRDAGVTLIVMLLA
                     LKTLELRARRDAMVVFFLGFFTLLANFFYSQALPIAALMLVALLGLLTALVNAHMPVG
                     RPPFLQAMRTAAHMMLLGAPLMALLFVLFPRMAPLWGLPVDKTSARTGLSAQMSIGSM
                     ASLVQDGSVALRVRFDGGEPPAPQELYFRGPVLTAFNGREWFALSQPEARAVAGWAAP
                     TAANLQVQGAPLGYELTLEPHQQTWLLTLDAAQAPPELPHGGRALMSPELQWSATRPI
                     HSVLRYRAESHLRFTHGPQRRTPQMQPYVQLPPELNPRTLALARQMQANPRLAAGGAQ
                     AFVDDALRRLRSGGYRYTLEPGLYGAHTADEFWFDRKEGFCEHIASAFVVLMRALDVP
                     ARIITGYQGGQQNPIDGYWTVHQSDAHAWAEVWIAGRGWVRVDPTSAVAPGRVGQLQR
                     LQAPRGMLGTAMDAVVSPTLVQHLRTVWEAANNGWNQWVLNYTQARQFDLLKTLGFAA
                     PSWLDLVRLLGLLICAAALAGALWALWERSRQDPWQRLLLKARARLARAGLALPAHLP
                     PRAMASQARERLGAPAEAAAQWLLRLEQTRYAPQPDATLGQLRREFTRLPWPTAQSRG
                     TGTP"
     gene            complement(3194..4234)
                     /locus_tag="PK518_14040"
     CDS             complement(3194..4234)
                     /locus_tag="PK518_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRM50077.1"
                     /translation="MPTTPGQAVNPAAPFPPTSPPARRRTGLLARWYRWWSARLPRTD
                     TLQLTQRNVFILPTGAGWMLALTLLVLLVASINFQLNLGYLLTFLLTGSAAASLYLCH
                     ATLRGLSLRLLVPEPQFLGAGAVLQVQLHNAARATRYGIGVAVRGSHQSREWVWCDVP
                     GQGSATVQVAFAPARRGLQGAPLLKLETRYPLGTFRSWALWRPAAQVLVYPRPETPAP
                     PLPPPEPHSSGPGQARARGLGEFDGVRAYQRGDPLKLVVWKKAAKSLATGSNDLVSRD
                     TQQSQRQQLWLDFAATGLPAPEDRLSRLTAWVLQADRLGQDYGLRLPGREIAPDHGPA
                     HRRRCLEALALC"
     gene            complement(4236..>4829)
                     /locus_tag="PK518_14045"
     CDS             complement(4236..>4829)
                     /locus_tag="PK518_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM50078.1"
                     /translation="ASPKTQSALLEAMEEKQVSVEGATRPLPQPFFVIATQNPHDQLG
                     TFALPESQLDRFLMRISLGYPDRASERQLLAGGDRRDMLTALPPLLSEAELAALQHQV
                     LAVHAAAPLLNYVQDLIAATRSGRWFVQGLSPRAGIALMRAAKAQALIEGRDYVAPDD
                     VQAILPQTIAHRLVPVGDAGRGSVEQVRAMLESVPLP"
BASE COUNT          706 a         1609 c         1809 g          705 t
ORIGIN      
        1 ctcgatcacc gccatggcgt agtagcttga ccagttgtag cgcgtgacgg cgtagaagtt
       61 ttcggtgccg gccacgtagc tcgctggtgc ggcgccgttt tgcagctcca ccagggccag
      121 cgggccggcg tacgtggcgc cgtcgccctg cacctgggcg cccttggcgg ccatgctggc
      181 cacgctgaag gtgggcagga tgtcgggggc cagcagctcg tcaagctgca ggctggcggt
      241 gtcgaaggcc acggcgtaat gcgtggccat gccgctgtgc cagccatggg ccacgaagta
      301 gttggccacc gagcctatgg cgtccggcgc gctgcgccac aggtcgatgc ggccgtcgcc
      361 gtcaaagtcc accgcccagc gtgaccagct cgatggcatg aactggccca ggcccatggc
      421 gccggcgtag ctgccgcgcg ggctgtgcgg gtcggtgccg gtgcgatcca tcagggacag
      481 gaactgctcc agctcgcgct ggaagaaggc gcgccgctcg ggcgcgcgcg ggtgggcgtc
      541 ggggaagtcg aaggcgaggg tggccagcgc gtcgatgacg cggtagccgc ccatgtgctg
      601 gccgtagatg gtctccacgc cgatgatgcc gacgatgatt tcggccggca cgccgtactc
      661 tttctcggcc cgcgccagcg tctcggcgtt gtcctgccag aagcgcaggc cggcgcgtat
      721 gcgcaccggc tcgacaaagc gcgcgcggta ggcggcccag ttcttgggtt ggcccttggg
      781 cggcggcagc accaggcgcg gtacctcgcg cagcaggcgt gcctggccta tggcctggcg
      841 cacccactcg cggtccaggc cgcggcgttc ggccaggtcg tcggcgaagc gcatggcttc
      901 ggcgctttcg gcgtaatgcg gggcgggggc tgccttggca gtctgttttt tagggttgtt
      961 tttggctgta gcgcttatgg ataaagcgca agcagctatc aaaaatgtag ttttgaaggt
     1021 caagaatgtc atggcgttcc tgtgccgcgc gattgtgccg tgggccaggg caggcgggtg
     1081 aattcgcgcc gcagctgtcc cagggtggcg tcgggttgcg gggcgtagcg tgtttgctcc
     1141 aggcgcagca gccattgcgc ggcggcctcg gcgggtgcgc ccagccgctc gcgcgcctgg
     1201 ctggccatgg cgcgcggcgg caggtgggcg ggcagtgcca ggccggcgcg tgccaggcgc
     1261 gcacgcgcct tcagcagcag ccgttgccag gggtcctggc ggctgcgctc ccacagcgcc
     1321 cacagggcgc cggccagggc ggcggcgcag atcagcaggc ccagcaggcg caccaggtcc
     1381 agccagctgg gtgcggcaaa gcccagggtc ttgagcaggt cgaactggcg tgcctgggtg
     1441 tagttgagca cccactggtt ccagccgttg ttggccgcct cccagacggt gcgcaggtgc
     1501 tgcaccaggg tggggctgac gacggcgtcc atggccgtgc cgagcatgcc gcgcggcgcc
     1561 tgcaggcgct gcaactggcc gacgcggccg ggggcgacgg cgctcgtcgg atccacgcgc
     1621 acccagccgc ggccggcgat ccagacctcg gcccaggcat gggcgtcgct ctggtgcacg
     1681 gtccagtaac cgtctatggg gttttgctgt ccgccctggt agccggtgat gatgcgcgcg
     1741 ggtacgtcca gcgcgcgcat cagcaccacg aaggccgagg cgatgtgctc gcaaaagccc
     1801 tctttgcggt caaaccagaa ctcgtcggcg gtgtgcgcgc catacaggcc gggctccagc
     1861 gtgtagcggt aaccgccgct gcgcagtcgc cgcagggcgt catcgacgaa ggcctgggcg
     1921 ccgccagcgg cgagccgggg gttggcctgc atctgtcgcg ccagggccag ggtgcgcggg
     1981 ttcagctcgg gcggcagttg cacatagggc tgcatctgcg gcgtgcgccg ctgcgggccg
     2041 tgggtgaagc gcaggtggct ctcggcgcgg tagcgcagca cgctgtggat ggggcgggtg
     2101 gcgctccact gtagctcggg cgacatcagc gcgcggccgc catggggcag ctcgggcggg
     2161 gcttgggcgg cgtccagggt cagcagccag gtttgctggt gcggctccag cgtcagttcg
     2221 tagcccagcg gcgcgccctg cacctgcagg ttggcggccg tgggcgcggc ccagccggcg
     2281 acggcgcgcg cctcgggttg cgacagggca aaccattcgc gcccattgaa ggccgtgagc
     2341 accgggccgc gaaagtacag ctcctgcggc gctggaggct cgccgccgtc aaagcgcacg
     2401 cgcagcgcca cgctgccgtc ctgcaccagc gacgccatgc tgcctatgct catctgcgcc
     2461 gacaggccgg tgcgcgcgct ggtcttgtcc acgggcaggc cccacaacgg ggccatgcgc
     2521 gggaacagca caaacagcag cgccatcagg ggcgcgccca gcagcatcat gtgcgccgcc
     2581 gtgcgcatgg cctgcaggaa cggcggccgg cccaccggca tatgtgcgtt gaccagcgcc
     2641 gtgagcaggc ccagcagcgc caccagcatc agcgcggcaa tcggcagcgc ctgcgaatag
     2701 aagaagttgg ccagcagcgt gaaaaaaccc aggaagaaca ccaccatggc gtcacgtcgt
     2761 gcgcgcaact ccagcgtctt cagcgccagc agcatgacga tcagggtcac gccggcgtcg
     2821 cgcccgacga tggtgccgtg cgacagcagc gtcagcagca ccaccagcgc cagcagcaag
     2881 gccgtgagcc agcgccctgg aagcggccga ccgcccacgg ccagccagcc gcgccaggcc
     2941 agcagcgtgg ccgtcatggc gctggcccag gccggcaggt gcgcggccag cggcgcgatg
     3001 cagcaggcga tgacggccag cagaaacagc gtgtcgcgcg catcgcgcgg caggcgcgac
     3061 agcggggcca ggcggccggc cagggtcatg gccggcctcc agtgcttttg tcgccagtct
     3121 tttcgctata taaaatatag ctgtttgcgc ttggtggcat tgcgttagtt gccaattttg
     3181 ttcatatttc cgatcagcac agcgccaggg cctccaggca gcggcgccgg tgtgccgggc
     3241 catggtcggg cgcgatctcc cgcccgggca ggcgcaggcc gtagtcctgc cccaggcggt
     3301 cggcctgcag cacccaggcc gtcaggcgcg acaggcgatc ctcgggcgcc ggcaggccgg
     3361 tggcggcaaa gtccagccac agctgctggc gctgggactg ctgggtatcg cggctgacca
     3421 ggtcgttgct gccggtggcc agcgacttgg cggccttctt ccagaccacc agcttcagcg
     3481 ggtcgccgcg ctggtaggcg cgcacgccgt caaattctcc caggccacgc gcgcgggcct
     3541 ggccggggcc gctgctgtgc ggctcaggtg gcggcagcgg cggtgcaggg gtctcgggcc
     3601 ggggataaac cagcacctgc gccgccgggc gccacagcgc ccagctgcga aaggtgccca
     3661 gcgggtagcg cgtctccagc ttcaacagcg gcgcgccttg caggccgcgc cgcgcgggcg
     3721 caaaggccac ctgcaccgtg gcgctgccct ggccgggcac gtcgcaccag acccattcgc
     3781 gcgactgatg gctgccgcgc acggccacgc ctatgccata gcgtgtggcg cgggcggcgt
     3841 tgtgcaactg cacctgcagg actgcgcccg cgcccaggaa ctgcggctcg ggcaccagca
     3901 ggcgcagcga caggccgcgc agcgtggcgt ggcacaggta caggctggcg gccgcgctgc
     3961 cagtcagcag aaaggtcagc agatagccca ggttgagctg gaagttgatc gacgccacca
     4021 gcagcaccag cagtgtcagc gccagcatcc agcccgcgcc ggtgggcagg atgaagacgt
     4081 tgcgctgggt cagctgcaat gtgtcggtgc gcggcaggcg cgcgctccac cagcggtacc
     4141 agcgcgcgag caggccggtg cggcgccttg ccggtgggct tgtcggagga aatggggccg
     4201 cggggttcac cgcctggcca ggggttgttg gcatgtcagg gcaggggcac ggattcgagc
     4261 atggcgcgca cctgctccac gctgccgcgc ccggcgtcgc ccacgggcac caggcggtgg
     4321 gcgatggtct gcggcaggat ggcctgcacg tcgtcgggcg ccacatagtc gcgcccctct
     4381 atcagcgcct gggccttggc cgcgcgcatc agcgcaatgc cggcgcgcgg cgacaggccc
     4441 tgcacgaacc agcgccccga gcgcgtggcg gcgatcaggt cctgcacgta gttgagcagc
     4501 ggagcggccg catgaacggc cagcacctgg tgctgcagag cggccagctc ggcctcggac
     4561 agcagtggcg gcagggcggt cagcatgtcg cggcggtcgc cgccggccag cagctggcgc
     4621 tcgctggcgc gatcggggta gcccagcgag atgcgcatca gaaagcggtc cagctggctc
     4681 tcgggcaggg caaaggtgcc cagctggtcg tgcgggttct gcgtggcgat gacgaagaag
     4741 ggctggggca gggggcgcgt ggcgccctcc accgagacct gcttttcctc catggcctcg
     4801 agcagcgcgc tctgcgtctt ggggctggc
//
LOCUS       DAOMTQ010000191         4805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4572_length_4805_cov_26.343368, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000191 DAOMTQ010000000
VERSION     DAOMTQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4572_length_4805_cov_26.343368"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1659
                     /gene="fumC"
                     /locus_tag="PK518_14050"
     CDS             268..1659
                     /gene="fumC"
                     /locus_tag="PK518_14050"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRM50079.1"
                     /translation="MTANTRTEHDSFGPIDVPADRLWGAQTQRSLLNFDISGERQPRE
                     LIHALAQVKRASARVNHGLGLLEARKQAAIVAAADEVIAGRHEDEFPLVVWQTGSGTQ
                     TNMNLNEVLANRASEIMGGPRGQARLVHPNDDVNKSQSSNDVFPTAMHVAAVQAIAER
                     LLPALALLRATLHAKAQAFDDIVKIGRTHLQDATPLTLGQEFSGWVAQLAHAERHVRD
                     ALPHLCELALGGTAVGTGLNAPPGYADQVAADLAAQTGLPFVTSPNKFESLASCDALV
                     AAHGALKTLAASLMKIANDVRWLASGPRSGIGEITIPENEPGSSIMPGKVNPTQCEAL
                     TMLAAQVLGNDVAINVGGSSGNFELNVFRPMVAHNFLQSVRLLADGMKSFNDHCAVGI
                     EPNRARIAELVERSLMLVTALNTHIGYDKAAQIAKKAHQDGSSLRQAALALGHVTAAQ
                     FDEWVVPGKMVGR"
     gene            complement(1886..2662)
                     /locus_tag="PK518_14055"
     CDS             complement(1886..2662)
                     /locus_tag="PK518_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM50080.1"
                     /translation="MNATLRPSPDAQSQRPLLTAREREAIGRSPWFAALTPSLRHDIL
                     RLGRVTRHAHGEAIVEQGQTMRDWFTCASGALRFRRTTPAGKQVTLAYVEPGVWVGEA
                     EVLYGRPCTYEAHAHGPTTLLSVPETALRSLLQQHPTFGEALLTLQAWSMRSLYTMME
                     DVATMPLRARLAKQLLHLLERFGAPYAEPQASRPLGLSLTQDELAGLLGGSRQRVNVE
                     LKWLEQQGLISVRPRGVEVHDAAGLQTLVDQAADEGKTGG"
     gene            complement(2761..3771)
                     /gene="proX"
                     /locus_tag="PK518_14060"
     CDS             complement(2761..3771)
                     /gene="proX"
                     /locus_tag="PK518_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HRM50081.1"
                     /translation="MPHLPQVLRQLAAATSLALLTLGAQAQALPGKGVTVLPLKSSIA
                     EETFQTLLVMKGLEQLGYDVQPIKEVEYPTAHIAIAQGDATFLADHWNPLHADYYKNA
                     GGDAKLFRKGVYSGNAAQGYLIDKKTADAHRISNIAQLKDPKIAALFDTNGDGKADLT
                     GCNPGWGCEAVIEHQLDAYGLRPTVTHNQGSYSALIADTITRFKAGKPVLYYTWTPYW
                     VSNELKPGKDVVWLEVPFSSLPGEQKGSNTQLPNGKNYGFVVNSQQIVANKAWAEKNP
                     AAARLFEVMRLPVADINAQNHLMSQGQNQAADIERHVNAWIKAHQKTFDGWLQQARAV
                     AH"
     gene            complement(3847..>4805)
                     /gene="proW"
                     /locus_tag="PK518_14065"
     CDS             complement(3847..>4805)
                     /gene="proW"
                     /locus_tag="PK518_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HRM50082.1"
                     /translation="APAPAGGDDASTGLAGLWQQLGSDGLPVQGWINDGLAWLVEHFR
                     PFFQTVRAPIDATLSGITDALLALPWPVMVLLITLLAWQFAGRALAVASAISLAAVVL
                     LGIWPDAMVTLALVLTSLLFCVVIGLPLGIWLASSDRAQRWMRPLLDAMQTTPAFVYL
                     VPVVMLFGIGNVPGVIVTIVFALPPLVRLTNLGLRQVRPDLVEASRAYGASPTQLLLK
                     VQLPLAMPSIMAGINQTLMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGLG
                     IVLLAIVLDRITQAMGEPRRGGQRWWHGGPLGLLLRKRTKKQ"
BASE COUNT          781 a         1520 c         1717 g          787 t
ORIGIN      
        1 gggtggtagt aggagatgta ctgcagcgct gcagctacgg actccacaag gtcgttgtaa
       61 cggatggtcg tggtcatgta ggcagggggt tgggggtgga acaaacccgg ggattatcgc
      121 ccccgagctt gcgtggggct tatgcgggga catgggcgcg gcgccgattg agcctactgc
      181 cggtaggggg cgggctttgc cctgcggggc tgctggggtg aaatggggct gcgccgccat
      241 cgccgccgcg gcagaaaggt ttacgccatg accgccaaca cccgcacaga gcacgacagt
      301 ttcggcccga ttgacgtgcc cgccgaccgg ctgtggggcg cgcagacgca gcgctcgctg
      361 ctgaacttcg acatctcggg cgagcgccag ccgcgcgagc tcatccacgc gctggcccag
      421 gtcaagcgcg cgtcggcgcg cgtcaaccat gggctgggcc tgctggaggc gcgcaagcag
      481 gcggccatcg tggccgcggc cgacgaggtg attgccggcc ggcatgagga tgagtttcca
      541 ctggtggtgt ggcagacggg ttcgggcacg cagaccaata tgaacctgaa cgaggtgctg
      601 gccaaccgcg ccagcgagat catgggcggc ccgcgcggcc aggcgcgcct ggtgcacccg
      661 aatgacgatg tgaacaagag ccagtcgagc aacgatgtct tccccacggc catgcatgtg
      721 gcggcggtgc aggccattgc cgagcggctg ctgccggccc tggccctgct gcgcgccacg
      781 ctgcacgcca aggcccaggc ctttgacgac attgtgaaaa ttggccgcac ccatttgcag
      841 gacgccacgc cgctgacgct ggggcaggag ttttcgggct gggtggcgca gctggcgcat
      901 gccgagcgcc atgtgcgcga tgcgctgccg cacctgtgcg agctggcact gggcggcacg
      961 gccgtgggca cggggctgaa tgcgccgccg ggctatgccg atcaggtggc cgccgacctg
     1021 gcggcgcaga ccgggctgcc gttcgtcacc tcgccaaata agttcgagtc gctggccagc
     1081 tgcgatgcac tggtggccgc gcatggcgcc ctgaaaaccc tggccgccag cctgatgaag
     1141 attgccaacg acgtgcgctg gctggccagc gggccgcgca gcggcattgg cgagatcact
     1201 atccccgaga atgagccggg ctccagcatc atgcccggca aggtcaaccc cacgcagtgc
     1261 gaggccctga ccatgctggc cgcgcaggtg ctgggcaacg atgtggccat caacgtgggg
     1321 gggagcagcg gcaacttcga gctgaacgtg ttccgcccca tggtggcgca caactttctg
     1381 caaagcgtgc gcctgctggc cgacggcatg aagagcttca acgaccattg cgccgtgggc
     1441 attgagccca accgcgcgcg catcgcggag ctggtggagc gctccctgat gctggtgacg
     1501 gcgctcaaca cccacatcgg ctacgacaag gccgcgcaga tcgccaagaa ggcgcatcag
     1561 gacggcagca gcctgcgcca ggcggcgttg gccctgggcc atgtcacggc ggcgcagttc
     1621 gacgaatggg tggtgccggg gaagatggtg gggcggtgag tgctggcgct tgatggtgca
     1681 aggcgtttct ttggcgcggc agtcactccc tctcccgcgt gcgggagagg gttggggtga
     1741 gggtggcaag gacggacgcc gcagccaatc accccctcac ccccgccctc tcccccaagg
     1801 gggcgaggga gtaaaagcca aagtgatatg ttgcgcccgc ccatacagcg cgaagcgcta
     1861 tcaaaaaagt aggtcgaggt gcgcctcagc cccccgtctt gccctcgtcc gcagcctgat
     1921 ccaccagggt ttgcaggccg gcggcgtcat gcacctccac gccacgcggg cgcacgctga
     1981 tcaggccttg ttgttccagc catttcagct ccacgttcac gcgctggcgc gagccgccga
     2041 gcaggccggc cagctcgtcc tgcgtcagtg acaggcccag gggacggctc gcctggggtt
     2101 cggcgtaggg ggcgccaaag cgctccagca gatgcagcaa ttgcttggcc aggcgcgcgc
     2161 gcagcggcat ggtggccacg tcttccatca tggtgtagag cgagcgcatg ctccacgcct
     2221 gcagcgtgag cagggcctcg ccgaacgtcg ggtgctgctg cagcagcgag cgcagcgccg
     2281 tctccggcac gctcagcagc gtggtcgggc catgggcatg ggcctcgtag gtgcaggggc
     2341 ggccgtagag cacctcggcc tcgcccaccc agacgcccgg ctccacatag gccagcgtga
     2401 cctgcttgcc cgccggcgtg gtgcgccgaa agcgcagcgc gccgctggcg caggtgaacc
     2461 agtcgcgcat ggtctggcct tgctcgacga tggcctcgcc atgcgcgtgg cgcgtgacgc
     2521 ggcccaggcg caggatgtcg tggcgcagcg atggcgtgag ggcggcaaac caggggctgc
     2581 gaccaatggc ttcgcgctcc cgggcggtca gcagcgggcg ttgagactgg gcatcgggac
     2641 tggggcgcag ggtggcgttc atgggcaggc aggcggcgcg gtgactgact gctcatttta
     2701 ggccgggctt gcatatgcgc cattgccgtg gcgcaaggcc tggcttgccg cggccgcctg
     2761 tcaatgcgcg acggcccggg cctgctgcag ccagccgtca aaggtcttct gatgcgcctt
     2821 gatccaggcg ttcacatggc gctcgatgtc ggcggcctgg ttctgaccct ggctcatcaa
     2881 atggttttgc gcgttgatgt cggccacggg caggcgcatc acttcaaaca gcctggcggc
     2941 ggccgggttc ttctcggccc aggccttgtt ggcgacgatc tgctggctgt tcacgacgaa
     3001 gccgtagttc ttgccgttgg gcagctgggt gttgctgccc ttttgttcgc caggcagtga
     3061 ggagaacggc acctccagcc agaccacatc cttgccgggt ttgagttcat tgctgaccca
     3121 gtacggcgtc caggtgtagt acagcaccgg cttgccggcc ttgaagcgcg tgatggtgtc
     3181 ggcgatcagc gccgagtagc tgccctggtt gtgcgtgacg gtggggcgca ggccgtaggc
     3241 gtccagctgg tgctcgatca ccgcctcgca gccccagccc gggttgcagc cggtgagatc
     3301 cgccttgccg tcgccattgg tgtcgaacag cgcggcgatc ttcgggtcct tgagctgggc
     3361 gatgttgctg atgcggtgtg cgtcggccgt cttcttgtcg atcagatagc cctgggcggc
     3421 gttgcccgag tagacaccct tgcgaaacag cttggcgtcg ccgcccgcgt tcttgtagta
     3481 gtcggcgtgc agcgggttcc agtggtcggc cagaaaggtg gcgtcgccct gggcgatggc
     3541 gatatgcgcc gtggggtact cgacctcctt gatcggctgc acgtcgtagc cgagttgttc
     3601 caggcccttc atcaccagca gggtctgaaa ggtttcctcg gcaatggagc ttttcagcgg
     3661 cagcactgtc acgcccttgc cgggcagcgc ctgggcttga gcgcccaggg tcagcagggc
     3721 aaggctggtg gcggcggcca gttggcgcag aacttggggc aggtgtggca tgtgaatctc
     3781 ctgagtgaaa attttggata aaaatgggct gtagcacttg tgtatcaagc gcggatagct
     3841 atggttttat tgtttcttgg tgcgcttgcg cagcagcagg cccagcggcc cgccgtgcca
     3901 ccagcgctgg ccgccacggc gcggctcgcc catggcctgg gtgatgcggt cgagcacgat
     3961 ggccagcagc actatcccta agccgccgac ggtggccagc cccatgtcca gccggccgat
     4021 gccgcgcagc accatctgcc ccaggccgcc gacggcgatc atcgaggcga tgaccaccat
     4081 ggacagactg agcatcagcg tctggttgat gccggccatg atggacggca tggccagcgg
     4141 tagctgcacc ttgagcagca actgcgtggg tgaggcgcca taggcgcggc tggcctcgac
     4201 caggtccggc cgcacttggc gcagacccag attggtcagg cgcaccagcg gcggcagcgc
     4261 aaacacgatg gtgacgatga cgccgggcac gttgccgatg ccaaacaaca tcaccaccgg
     4321 aaccagatag acgaaggccg gcgtggtctg catggcgtcc agcagcggac gcatccagcg
     4381 ctgggcgcgg tcgctgctcg ccagccagat gcccagcggc aggccgatca ccacgcagaa
     4441 cagcagcgag gtgagcacca gcgccagcgt gaccatggca tcgggccaga tgcccagcaa
     4501 caccacggcg gcgagcgaga tggcgctggc gacggccagt gcgcggccgg cgaattgcca
     4561 ggccagcagc gtgatgagga gcaccatcac cggccagggc agggccagca gcgcgtcggt
     4621 gatgccgctg agcgtggcgt cgatgggcgc gcgcacggtc tggaagaagg ggcgaaagtg
     4681 ctccaccagc caggccaggc cgtcgttgat ccagccctgc acgggcaggc cgtcgctgcc
     4741 gagctgctgc cacaggccgg ccaggccggt gctggcgtca tcgccgccgg ctggcgcggg
     4801 cgcgg
//
LOCUS       DAOMTQ010000192         4774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4621_length_4774_cov_17.004662, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000192 DAOMTQ010000000
VERSION     DAOMTQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_4774_cov_17.004662"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PK518_14070"
     CDS             <1..955
                     /locus_tag="PK518_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM50083.1"
                     /translation="PDDLQDASAAERALALQIVWDIRLPRTLGAWAAGALLGLAGAVA
                     QGLFRNPLADPYLLGSASGASLGVAAVLALMGSVGFLGTGRGALAASVYSGQWLVRLG
                     LTGAAFVGAVAAVLLTLALARGVQHTLRLLLAGVVVGVVLGACTSLILLLSPDSLQAM
                     QAFLLGSTGFVGWTSCLLLLAVWLPCWLVAWALSRALDALALGESTAHSLGLPLAQQR
                     AALVVVLALATGTAVAQTGLIAFVGLAAPHLVRAAVRVTHGRLMLLASLMGGALLCAA
                     DLLARGLLAPQELPVGVLTAVLGGGYLLWLMHRKTAAGGGL"
     gene            1021..1728
                     /locus_tag="PK518_14075"
     CDS             1021..1728
                     /locus_tag="PK518_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM50084.1"
                     /translation="MHGVDVRLAAGRWTSIVGPNGAGKSTLLKALAGLLPVAGGGVQL
                     LGRDLHAWPRRERAQAMAWLGQNEASADDLRVYDVAMLGRLPHQGFLQPASSADHAAV
                     EQALRATQAWDWRERPLSQLSGGERQRVLLARALAVQARVLLMDEPLANLDPPHQADW
                     MQTVRALVARGTTVVSVLHELGMALAADELVVMQGGRVLMQGACADAATHRAVEQVFD
                     RRVAVHSLQGQWVALPL"
     gene            1795..2472
                     /locus_tag="PK518_14080"
     CDS             1795..2472
                     /locus_tag="PK518_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRM50085.1"
                     /translation="MHIEPAVVEGAKIALSYASGTAALGYAAKLALQAMKTDGALALL
                     VRTLIAAVAVFISFEVLPHTPVGVSEVHLILGTTLLLMLGVAPAALGLAAGLLVQSLF
                     FAPQDLPQYGMNVTTLLVPLFATAALASRIIPRATAYVDLSYAQTLKLSLAYQGGIVV
                     WVAFWALYGHGVGAANLQNIATFGVAYMGVVLLEPLIDLAVLAAAKALRGLQGSALVQ
                     SRVYSAA"
     gene            2592..3161
                     /gene="cobO"
                     /locus_tag="PK518_14085"
     CDS             2592..3161
                     /gene="cobO"
                     /locus_tag="PK518_14085"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRM50086.1"
                     /translation="MEIETPPIHRDTPKPQGERRGLVLVHTGTGKGKSTAAFGLALRA
                     HGRGKAVKVYQFMKVPSARFGEHRLFEQLGIPIEGLGDGFSWKSRDLDHSAQLAKDGW
                     ARAEASIRAGELFMVVLDEIMYPLRYGWIALDDVLACLRERPGHVHVVLTGRGAPAEL
                     VELADTVTEMTLIKHHFKAGVPAQRGIED"
     gene            3161..4009
                     /locus_tag="PK518_14090"
     CDS             3161..4009
                     /locus_tag="PK518_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRM50087.1"
                     /translation="MAHLPHEQRSLNRSAALALLCALAAPGQAQQRIVTVAPSLTESV
                     CALGHCGQIVGTDRYSTGPGPVAQLPKVGGLADASIEHIVALRPDLVLLGPRTRLDDR
                     LKQLGIAVQTFNVRTHAEQKAMLRALGRTLGESARAESLIAQVERELDAAAARLPAQL
                     RGRSVYVEIGLGPKAASEATFIGETLARLGLVNIVGAEQGFFPRVNPEFIVRRAPDLI
                     IGPAALLANLHQRPGWERLPAVRRQQVCALDPERMALLERPGPRLGEAAQMLVDCLRQ
                     LPGVPH"
     gene            4038..>4774
                     /gene="cobW"
                     /locus_tag="PK518_14095"
     CDS             4038..>4774
                     /gene="cobW"
                     /locus_tag="PK518_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HRM50088.1"
                     /translation="MHLRKIPATIVTGFLGAGKTTLLRHLLANADGRRIAVIVNEFGE
                     LGIDGELLRACGIGCDESGQDNGQLFELANGCLCCTVQEEFAPVMEQLAARRDAIDHL
                     IIETSGLALPKPLVQAFQWPQIRNAFTVDAVVTVVDAPAVLAGQFAEDPVAVDAQRRA
                     DDNLDHESPLAELFEDQLATADLVVLNKLEGLDADAVAQVEALVRAETPAGVQLVHAA
                     QGRVAPEVLLGQGRAVEDLIDARRTHH"
BASE COUNT          629 a         1650 c         1717 g          778 t
ORIGIN      
        1 cccagacgat ttgcaggacg ccagcgccgc cgagcgcgcg ctggcgctgc aaatcgtctg
       61 ggacatccgc ctgccgcgca cgctgggcgc ctgggccgcg ggcgcgctgc tggggctggc
      121 cggcgccgtc gcccaggggt tgtttcgtaa cccgctggcc gacccctatc tgctgggcag
      181 cgcctcgggc gcgtcgctgg gcgtggcggc ggtgctggcg ctgatgggca gcgtgggatt
      241 tttgggcacc gggcgcgggg cgttggcggc gtcggtctat tcgggccagt ggctggtgcg
      301 cctgggcctg acgggcgcgg ccttcgttgg cgcggtggcc gccgtgctgt tgacgctggc
      361 gctggcgcgc ggcgtgcagc acacgctgcg cctgctgctg gccggcgtgg tggtgggcgt
      421 ggtgctgggc gcgtgcacct cgctcatctt gctgctgtcg cccgattcgc tgcaggccat
      481 gcaggcgttt ttgctcggct ccaccggctt cgtcggctgg acttcctgcc tgctgctgct
      541 ggccgtgtgg ctgccctgct ggctggtggc ctgggcgctt tcgcgcgcgc tggacgcgct
      601 ggcgctgggc gagtccaccg cgcacagcct gggcctgccc ttggcgcagc agcgcgcggc
      661 gctggtggtg gtgctggcgc tggccaccgg cacggcggtg gcgcagaccg ggctgatcgc
      721 cttcgtcggc ctggccgcgc cgcacctggt gcgcgcggcg gtgcgcgtga cgcacgggcg
      781 gctgatgctg ctggccagcc tgatgggcgg cgccctgctg tgcgccgccg acctgctggc
      841 gcgcggcctg ttggcaccgc aggagctgcc ggtgggcgtg ctgaccgcgg tactgggcgg
      901 cggctacctg ctgtggctga tgcaccgcaa gacggcggcg ggaggtgggc tgtgattgct
      961 cctgaaaaaa tagctatcaa cgcatgccag ataagcgttt ccatcggaaa agcccaaata
     1021 ttgcacggtg tggatgtgcg gctggcggcg gggcgttgga cgagcatcgt cggccccaac
     1081 ggcgcgggca agtccacctt gctcaaggcg ctggccggcc tgctgccggt tgccggcggc
     1141 ggcgtgcagc tcttggggcg cgatctgcac gcctggcccc ggcgcgagcg cgcccaggcc
     1201 atggcctggc tggggcagaa cgaggccagc gccgacgacc tgcgcgtgta cgacgtggcc
     1261 atgctcgggc gcctgccgca ccaaggtttt ttgcaacccg ccagcagcgc cgatcacgcc
     1321 gccgtggagc aggcgctgcg cgccacgcag gcctgggact ggcgcgagcg gccgctgtcg
     1381 cagctctcgg gcggcgagcg ccagcgcgtg ctgctggcgc gcgcactggc ggtgcaggcg
     1441 cgcgtgctgt tgatggacga gccgctggcc aacctggatc cgccgcacca ggccgactgg
     1501 atgcagacgg tgcgcgcgct ggtggcgcgc ggcaccacgg tggtcagcgt gctgcacgag
     1561 ctgggcatgg cgctggcggc cgacgagctg gtcgtgatgc agggcgggcg cgtgctgatg
     1621 cagggcgctt gcgccgacgc ggccacgcac cgcgcggtgg agcaggtatt cgaccggcgc
     1681 gtcgccgtgc attcgctgca gggccaatgg gtggccttgc cgttgtgatg gatttttgat
     1741 tttcaacccg cgccctggca gccgttgggg cgcactactt gtgaggagac tgccatgcat
     1801 atcgaacccg cagttgtcga gggcgccaag atcgccctga gctacgccag cggcacggcc
     1861 gcgctgggct atgcggccaa gctggcgctg caggccatga agaccgacgg cgcgctggcg
     1921 ctgttggtgc gcacgctgat cgctgcggta gcggtgttta tatcgttcga ggtgctgccg
     1981 catacgccgg tgggcgtctc cgaggtgcac ctgattctgg gcaccacgct gctcttgatg
     2041 ctgggcgtgg cgccggctgc cctgggcctg gccgcgggcc tgctggtgca aagcctgttc
     2101 tttgcgccgc aagacctgcc gcagtacggc atgaacgtga cgacgctgct ggtgccgctg
     2161 tttgccaccg cggcgctggc cagccgcatc atcccgcgtg ccaccgccta cgttgacctg
     2221 agctatgcgc agacgctcaa gctctcgctg gcctaccagg gcggcatcgt cgtctgggtg
     2281 gccttctggg cgctctacgg ccacggcgtg ggcgccgcca acctgcagaa catcgccact
     2341 tttggcgtgg cctatatggg cgtggtgctg cttgagccct tgatcgacct ggccgtgctc
     2401 gccgccgcca aggcgctgcg cggcctgcag ggcagcgcgc tggtgcaaag ccgcgtgtac
     2461 agcgcggcgt gactttgatc tccctccccc tctgggggag ggctggggtg ggggccaacg
     2521 gcgcgccctg gcccccatcc ccaccttccc ccagaggggg aaggagtaaa gacaactcct
     2581 cattgaatcc catggaaatc gaaactcccc ccattcatcg cgacaccccc aagccccagg
     2641 gcgaacgccg tggcctggtg ctggtgcaca ccggtaccgg caagggcaag agcacggccg
     2701 cctttggcct ggcgctgcgc gcgcacgggc gcggcaaggc cgtcaaggtg tatcagttca
     2761 tgaaggtgcc ctcggcgcgc tttggcgaac accgcctgtt cgagcagctg ggcattccca
     2821 tcgagggcct gggcgatggc tttagctgga agagccgcga cctggaccat tcggcgcaac
     2881 tcgccaagga cggctgggcc agggccgagg ccagcatccg cgcgggcgag ttgttcatgg
     2941 tggtgctcga cgaaatcatg tacccgctgc gctacggctg gattgcgctc gatgacgtgc
     3001 tggcctgcct gcgcgagcgc cccggccatg tgcacgtggt gctgaccggg cgcggcgcgc
     3061 cggccgagct ggtcgagctg gccgacaccg tgaccgagat gaccctcatc aagcaccact
     3121 tcaaggccgg cgtgccggcg cagcgcggca tcgaggactg atggcgcacc tccctcacga
     3181 gcagcgctcc ctgaacaggt ctgcggcgct ggctttgctg tgcgcgctgg ccgcgccggg
     3241 ccaggcgcag cagcgcatcg tcacggtggc gccctcgctc accgaatcgg tgtgcgcgct
     3301 gggccactgc ggccagatcg tcggcaccga ccgctattcc accggccccg gccccgtggc
     3361 ccaactgccc aaggtgggcg ggctggccga cgcgtcgata gagcacatcg tcgccctgcg
     3421 cccagacctg gtgctgctcg gcccgcgcac ccggctcgac gaccgcttga agcagctggg
     3481 cattgccgtg cagaccttca acgtgcgcac ccacgccgag caaaaggcca tgctgcgcgc
     3541 gctgggccgc accctgggcg agagtgcgcg cgcagaaagc ctgatcgcgc aggttgagcg
     3601 cgagctggac gccgccgccg cgcgcctgcc cgcgcagcta cgcgggcgca gcgtgtacgt
     3661 ggagataggc ctcggcccca aggcggcgag cgaggccacc ttcatcggcg agacgctggc
     3721 gcggttaggc ctggtgaaca tcgtcggcgc ggagcaggga tttttcccgc gcgtcaaccc
     3781 cgagttcatc gtgcgccgcg cgcccgatct catcatcggc cccgccgccc tgctggccaa
     3841 cctgcaccag cgccccggct gggagcgcct gcccgccgtg cgccgccagc aggtctgcgc
     3901 gctcgacccc gagcgcatgg cgctgctcga gcgccccggc ccgcgcctgg gcgaggcggc
     3961 gcagatgctg gtcgattgcc tgcgccagct acccggcgtg ccgcattgaa tccctttttt
     4021 gaatcctgga gcctttcatg cacctgcgca aaatccccgc caccatcgtc accggctttc
     4081 tcggcgccgg caaaaccacg ctgctgcgcc acctgctggc caacgccgat ggccggcgca
     4141 tcgccgtcat cgtcaacgag tttggcgagc tgggcataga cggcgagctg ctgcgcgcct
     4201 gcggcattgg ctgcgacgag tcgggccagg acaacggcca gctttttgaa cttgccaacg
     4261 gctgcctgtg ctgcacggtg caggaagaat ttgccccggt gatggagcag ctcgccgcgc
     4321 ggcgcgatgc catcgaccac ctgatcatcg agacctcggg cctggcgtta cccaagcccc
     4381 tggtccaggc cttccagtgg ccgcagatcc gcaacgcctt caccgtggac gccgtggtca
     4441 ccgtggtcga tgcgcctgcc gtgctcgccg gccagtttgc cgaagacccg gtggccgtcg
     4501 atgcccagcg ccgcgccgac gacaacctgg accacgaatc ccccctggcc gagttgtttg
     4561 aagaccagct cgccacggcc gacctggtgg tgctcaacaa gctcgaaggg ctggacgccg
     4621 atgctgtcgc ccaggtcgaa gccctggtgc gcgccgagac gcccgccggc gtgcagctgg
     4681 tgcacgccgc ccagggccgc gtcgcccccg aggtgctgct cggccagggc cgcgcggtgg
     4741 aagacctgat cgacgcgcgc cgcacgcacc acga
//
LOCUS       DAOMTQ010000193         4740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4671_length_4740_cov_19.297759, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000193 DAOMTQ010000000
VERSION     DAOMTQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_4740_cov_19.297759"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /gene="gspN"
                     /locus_tag="PK518_14100"
     CDS             <1..682
                     /gene="gspN"
                     /locus_tag="PK518_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HRM50089.1"
                     /translation="ATLPFAIAGTVLAIGFVVSFNSGPLVLTGGAGSEDQAALPGRVR
                     WRLRPAWGGAQAEIAADCCTAQPLTLRAALGLGALQLQITDGQSQWPAGVLAGLGTPW
                     NTVQPQGRLQLVTTGLALQWAAGRMQLQGQARLDALDMSSRLSTLRPMGSYRLLLQGG
                     SGGQAPQLTLSTLDGALLLSGNGQWVGQRLRFTGEASAAPERAAALANLLNIIGRRSG
                     ARSIITLG"
     gene            687..3017
                     /gene="gspD"
                     /locus_tag="PK518_14105"
     CDS             687..3017
                     /gene="gspD"
                     /locus_tag="PK518_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HRM50090.1"
                     /translation="MPFSSRHPWRFALHSIAVYALLVCANGQINAQTAAKSNPPSVRA
                     SEPVTLNFANAEIEAVARTMATITGRNVVVDPRVKGQITLVTENAVLPAAAFQQFQAA
                     LRLQGFTVVESGGLYKVVPEADAKLQTGAVSVTQGSSGSAPAGGQMVTQIFKLNFENA
                     NNLVPVLRPLISPNNTINVNPGNNSLVITDYADNLQRLARIIAAMDVSNATDLEVIPL
                     QHALASDLAPLVARLIEGTGSTGAAPAAAQGQTDTAFKTTLLAEPRSNALIVRAANPA
                     RLALVRSLVAKLDQPAAPGSSAASGNIHVVYLKNADAVKLAATLRAALQASNPGGGAG
                     ATLSGGTGVSGLAQTSPSGGMGNQSSLSSSAMGSSTGGGLGSGTATGLNRGGNSNQPS
                     TGGQIQADPTTNSLIISAPEPQYRQLRAVIDKLDGRRAQVLIESLIVEVSANKVAEFG
                     IQWQGVMGNHGSTLGVLGTNSSVAGTNILDLALAAASGNVSSLAAAKPSAGMNFALAP
                     RINGKYYLGALANFLENTGDANVLSTPNLMTLDNEEAQIIIGNNVPFPTGSYTNTGGN
                     NGAVNPFTTVERKDVGLMLRVRPQINENGTVKLTLYQEVSTIAEGTIGNVNGPSTRKR
                     SIESTVMVDDGNVIVLGGLLEDNYTLNQDKVPVMGDLPILGNLFRNEKRTRQKSNLMV
                     FLRPLVIRENITNDALVIDRYEAIRAQQKIIQPEPNSIMRSVSDAPLLPQLLPKTTSP
                     FTQRSNPPSIPGETTIRQNSYPDLLSPDSAPNTGDH"
     gene            3413..>4740
                     /gene="gspE"
                     /locus_tag="PK518_14110"
     CDS             3413..>4740
                     /gene="gspE"
                     /locus_tag="PK518_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HRM50091.1"
                     /translation="MRHPLPYAFARSSQLLLEDDGQQLVLWHGPQPDAGALSEVLRKH
                     AVQQLMQIDAAPLAQRISAAYAQSESSAATVVSEVEEEADLSRMMQELPAVEDLLESA
                     GDAPIIRMLNALLTQAVRDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALI
                     SRLKIMADLDISEKRLPQDGRISLRLGTRAIDVRVSTLPNAHGERAVLRLLDKSESKL
                     SLESVGMQGEALTRFEGLIAQPHGIILVTGPTGSGKTTTLYAALSRLDATRSNIMTVE
                     DPIEYELPGVGQTQVNAKIDLTFAKALRAILRQDPDVIMIGEIRDFETAQIAIQASLT
                     GHLVLATLHTNDAASAITRLTDMGVEPFLLSSSLLGVLAQRLVRKYCGHCHGGPHGTG
                     CEHCGHTGYAGRTGVFELLVVNDAIRAQIHGQAAEAEVRAQALAGGMQLMR"
BASE COUNT          961 a         1615 c         1443 g          721 t
ORIGIN      
        1 ggccaccctg cccttcgcca tcgccggcac cgtgctggcc atcggtttcg tcgtctcgtt
       61 caacagcggc ccgctggtgc tgaccggcgg cgccggcagc gaagaccagg ccgccctgcc
      121 cgggcgcgtg cgctggcgcc tgcgccccgc ctggggcggc gcccaggcag agattgcggc
      181 cgattgctgc acggcccagc cgctcacgct gcgcgccgcg ctaggcctgg gcgcactgca
      241 actgcagatc accgatggcc agagccaatg gccggccggc gtgctggccg gcctgggcac
      301 gccctggaac accgtacagc cacagggccg gctgcagctg gtcaccaccg gcctggccct
      361 gcaatgggcg gccgggcgca tgcagctgca gggccaggcg cggctggatg cgctggacat
      421 gtcctcgcgc ctgtccacgc tgcggcccat gggcagctac cggctgctgc tgcagggcgg
      481 ctcgggcggc caggcaccgc agcttacgct gtccacgctg gatggcgcgc tgctgctgtc
      541 gggcaacggc cagtgggtgg ggcagcgcct gcgttttacc ggcgaggcca gcgccgcgcc
      601 cgagcgcgcg gcggcgctgg cgaatcttct gaacatcatc ggacggcgca gcggcgcgcg
      661 atccatcatc accctggggt gaacctatgc ctttctcttc ccgacaccca tggcgttttg
      721 ctcttcattc gatagctgtt tacgctttac tggtatgcgc taacggccaa ataaatgcac
      781 aaactgcagc caaatccaat ccgcccagcg tgcgcgccag cgagccggtg acgctgaact
      841 tcgccaacgc cgagatcgag gccgtggcgc gcaccatggc caccatcacc gggcgcaacg
      901 tggtggtcga tccacgcgtc aagggtcaaa tcaccctggt gaccgagaac gccgtgctgc
      961 cggcggcggc cttccagcag ttccaggcgg cgctgcgcct gcagggcttc acggtggtcg
     1021 aatcgggcgg gctgtacaag gtggtgcccg aggccgacgc caagctgcag accggcgccg
     1081 tgagcgtgac ccagggcagc agcggcagcg cccccgcggg cgggcagatg gtgacgcaga
     1141 tcttcaagct caacttcgag aacgccaaca acctggtgcc ggtgctgcgc cccctgatca
     1201 gcccgaacaa caccatcaac gtgaaccccg gcaacaactc gctggtgatc accgactacg
     1261 ccgacaacct gcagcggctg gcgcgcatca tcgccgccat ggatgtctcc aacgccaccg
     1321 acctggaggt gataccgctg cagcatgcgc tggcctccga cctggcgccc ctggtggcgc
     1381 ggctgatcga gggtacgggc agcaccgggg ccgcaccggc cgcggcccag ggccagaccg
     1441 acaccgcctt caagaccacg ctgctggcgg agccacgcag caacgcgctg atcgtgcgcg
     1501 cggccaatcc cgcgcggctg gcgctggtgc gctcgctggt ggccaagctg gaccagccgg
     1561 ccgcgcccgg cagctcggcc gccagcggca acatccatgt ggtctacctg aagaatgccg
     1621 atgccgtgaa gctggccgcc acgctgcgcg ccgcgctgca ggcaagcaat ccgggcggcg
     1681 gcgccggcgc caccttgagc ggcggcacgg gcgtcagcgg cctggcgcaa accagcccgt
     1741 caggcggcat ggggaaccaa agcagcctga gcagcagcgc catgggcagc agcaccggcg
     1801 gcggcctggg ctcgggcacg gccacgggcc tgaaccgcgg cggcaacagc aaccagccca
     1861 gcacgggcgg acagatacag gccgacccga cgaccaactc gctcatcatc agcgcgcccg
     1921 agccgcagta ccgccagctg cgcgccgtga tcgacaagct ggacggccgg cgcgcccagg
     1981 tgctgatcga gagcctgatc gtcgaggtga gcgccaacaa ggtcgccgag ttcggcatcc
     2041 agtggcaggg cgtgatgggc aaccacggca gcacgctggg cgtgctgggc accaattcga
     2101 gcgtggcggg caccaacatc ctggatctgg cgctggccgc ggccagcggc aacgtcagct
     2161 cgctggcggc ggccaagccc tcggccggca tgaacttcgc actggcaccg cgcatcaacg
     2221 gcaagtacta cctgggggcg ctggccaact ttctggagaa caccggcgac gccaacgtgc
     2281 tgtccacccc caacctgatg acgctggaca acgaagaggc gcagatcatc atcggcaaca
     2341 acgtgccctt ccccaccggc tcatacacca acaccggcgg caacaacggc gccgtgaacc
     2401 ccttcaccac cgtggagcgc aaggacgtgg gcctgatgct gcgcgtgcgc ccgcagatca
     2461 acgagaacgg caccgtcaag ctgacgctgt accaggaggt gtcgacgatc gccgagggca
     2521 cgatcggaaa cgtaaacgga ccctcaacgc gcaagcgctc cattgaatcc actgtaatgg
     2581 tcgatgacgg caacgtaatc gtgttgggcg gtcttctaga agataactac acacttaatc
     2641 aggataaggt acctgtgatg ggagatttgc ccatactagg caatctattc cggaacgaaa
     2701 aacgaacccg ccagaaatcc aacctaatgg ttttcttgcg tcctctagtg atccgtgaaa
     2761 atattaccaa cgatgcccta gtgattgatc gctacgaggc catccgagca cagcaaaaaa
     2821 taattcaacc tgagccaaat tccattatgc gctcagtttc tgatgcccca ctcctaccgc
     2881 agcttctacc aaaaacaacc tcccccttca cacaacgatc aaacccacca tcgataccag
     2941 gagaaacaac gatacgacaa aattcatacc ctgatttact atcgcccgac agtgccccaa
     3001 acactggaga tcattaaaaa tcatgaaata cattaaatat ataatttcga tttatataat
     3061 tgccgcattt tcttccgcac atgcagccgg cgaagtagta caaagctgct caggatacgg
     3121 accttatggc aatataatac ccggatgggc attgataagc ataaaaacat atgcagcagg
     3181 accatgcgga ataccttcta gcactcaata cacctggaag aatatcaatg gatatcctta
     3241 tggcagtcgc gaaattgttt gcagctttga gaatctccca aattggtggc caattcaata
     3301 tattagaaat accagccctg cccaaggtcc ctgcaacggc gctgcataca cttacctcgt
     3361 agagtacaga ggaaattaat caaccaaata atccaagatc attcctgcaa atatgcgcca
     3421 ccccctgccc tacgcctttg cgcgcagcag ccagctgctg ctggaggacg acggccagca
     3481 gctcgtgctg tggcacggcc cgcagcccga cgccggagcg ctgtccgagg tgctgcgcaa
     3541 gcacgccgtg cagcagctca tgcagatcga cgccgcgccg ctggcccagc gcatcagcgc
     3601 cgcctatgcg caaagcgaat cgagtgccgc caccgtggtc agcgaggtcg aggaagaggc
     3661 ggatctgtcg cgcatgatgc aggagctgcc cgcggtcgag gatctgctcg aatcggccgg
     3721 cgacgcgccc atcatccgca tgctcaacgc cctgctgacc caggccgtgc gcgacggcgc
     3781 gagcgacatc cacatcgagc cctacgagcg ccattccagc gtgcgctttc gcgtggacgg
     3841 cacgctgcgc gaggtggtgc agcccaatcg cgcgctgcac gcggcgctga tcagccggct
     3901 gaagatcatg gcggatctcg acatctcgga gaaacgcctg ccgcaggacg ggcgcatcag
     3961 cctgcgcctg gggacaaggg ccatcgacgt gcgcgtctcc accctgccca acgcccatgg
     4021 cgagcgcgcc gtgctgcgcc tcctggacaa gagcgaaagc aaattgagcc tggaatccgt
     4081 gggcatgcag ggcgaggcac tgacgcgttt cgagggcctg atcgcccagc cgcacggcat
     4141 catcctggtc accggcccca cgggttcggg caagaccacc acgctgtacg cggcgctctc
     4201 gcgactggac gccacgcgca gcaacatcat gacggtggaa gaccccatcg aatacgagct
     4261 gcccggcgtg ggccagaccc aggtcaacgc aaaaatagac ctgaccttcg ccaaggccct
     4321 gcgcgccatc ctgcgccagg acccggacgt gatcatgatc ggcgagatcc gcgacttcga
     4381 gaccgcgcag atcgccatcc aggccagctt gaccggccac ctggtgctgg ccacgctgca
     4441 caccaacgac gcggccagcg ccatcacgcg cctgaccgac atgggggtgg agccgttttt
     4501 gctgtcctca tccctgctcg gcgtgctggc ccagcgcctg gtgcgcaaat actgcggcca
     4561 ctgccatggc ggcccccatg gcacgggctg cgagcattgc ggccacaccg gctacgccgg
     4621 gcgcaccggc gtgttcgagc tgctggtagt gaatgacgcc atccgcgccc agatccacgg
     4681 ccaggcggcc gaggccgagg tgcgcgccca ggccctggcc ggcggcatgc agctgatgcg
//
LOCUS       DAOMTQ010000194         4679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4771_length_4679_cov_26.906791, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000194 DAOMTQ010000000
VERSION     DAOMTQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_4679_cov_26.906791"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PK518_14115"
     CDS             <1..639
                     /locus_tag="PK518_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM50092.1"
                     /translation="ASHELRSPLTRIRMGLELMQGGQPSPAFRAEIERNITELDQLVD
                     EILLASRLDAGAADVGTVEPVDLVGLAVEECVRVDAELQVGAGGADDDGAATIEVPGV
                     SKLLRRAVRNLLENARRYSQGEITLHITRDGSTAVLRVCDLGPGVPPDQRERIFEPFY
                     RLPGASERAGGVGLGLALVRSIAARHGGSVVCEGRPDGAAGACFVLRLPTTN"
     gene            875..1675
                     /locus_tag="PK518_14120"
     CDS             875..1675
                     /locus_tag="PK518_14120"
                     /inference="COORDINATES: protein motif:HMM:NF038132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038132 family protein"
                     /protein_id="HRM50093.1"
                     /translation="MKAFQLMGLAIGALLGAGTAAAGPLPGGWSCTGNCGTNTAADGD
                     VTLAPGFGSYQWISTAGGLTGAGQLPVGPTGQETNGSSAQTPTFSVNAGDKLNFYFNY
                     ITSDGVQFTEYAWAGLYSGTSTFDSYLFTARTTTSGNTVPGFGLPGLGAGVTLTPSTS
                     AIIPGGPDFSPLGGSSGGCYGGSGQGCGYTGWIQMEYTFTTAGIYSLGFGVTNALDTA
                     FDSAFAIAGVSINDTPIDPNPVPEPATLTLLGASLLGLGALRRRRQQG"
     assembly_gap    1887..1986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1987..2190
                     /locus_tag="PK518_14125"
     CDS             <1987..2190
                     /locus_tag="PK518_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRM50094.1"
                     /translation="GFLEQGSRFLNPVFVGDTLYCTLTVSDLKPGNTTGVITLGTEVK
                     NQKGVVVMDGFQKYLLRKRHPGQ"
     gene            2315..2809
                     /locus_tag="PK518_14130"
     CDS             2315..2809
                     /locus_tag="PK518_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRM50095.1"
                     /translation="MSTADIGTLQRTPTGFEGQLTRQLDHDAVAVWRMLTDPLAMAQW
                     LAPGSVEQRAGGTVHIDFADSGTVIDSRVSAFEDGRLLAYSWSSGSEPERPMRWELVP
                     ANGGTRLTLTLHIPAQEDAAKACAGFEGHLDMLAAALEGVPIKFPFQRFLQARGAYTE
                     QLAG"
     gene            2818..3378
                     /locus_tag="PK518_14135"
     CDS             2818..3378
                     /locus_tag="PK518_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRM50096.1"
                     /translation="MTSSAYHPAAPPLQVAQWFNTREPLSLESLRGRVVLLHAFQMLC
                     PGCVEHGIPQARRVHEAFSPDQVTVIGLHSVFEHHAVQGTPAALQAFIHEYRLRFPIA
                     LDQPHPTRNIPLTMQELMLQGTPSVVVIDAQGRVRLHHFGHLDDLRLGALLGQLVGEA
                     TAPVASAPAAPGCDDHGCARPDLPQS"
     gene            3375..4091
                     /locus_tag="PK518_14140"
     CDS             3375..4091
                     /locus_tag="PK518_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRM50097.1"
                     /translation="MNSTPPQAVAKLRALLTGRALPYTRPGSVSAIAKQPRSGPVAVG
                     LLGLADDEQGDPRVHGGPDKAVHCYAFSQYAPWRQELAGNATAQALLAQPGAFGENFS
                     LDGINESQVCLADQWQVGSARFEVSQGRQPCWKLNDRFGLPDMALRVQTSLRAGWYLR
                     VLQVGQVQAGDTVWLLARPHPEWPIDRLLRVIAERDCAPETLREILTLPLPPSWHRLF
                     TRRLESASVEDWSSRLVGKP"
     gene            complement(4095..>4679)
                     /locus_tag="PK518_14145"
     CDS             complement(4095..>4679)
                     /locus_tag="PK518_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRM50098.1"
                     /translation="AAGGGAAAARGVHALAYGLAGLGYIVTATFLPVIARGALPAGSP
                     WPDLFWPMFGAGVAVGAALSTRAPTRWDRRWLLVAAYAMQAGGIGIGLWWPTPAGFAL
                     SSILLGLPFTAITFYGLQEARRLWPASADSFASLITAVYGLGQIMGPPMVAWLLARGG
                     QAQGFAQGLALAAAALVAGALMYLASVWRWPLVR"
BASE COUNT          780 a         1628 c         1473 g          698 t
ORIGIN      
        1 gcctcgcacg agctgcgctc gccgctcacg cgcatccgca tggggctgga gctgatgcag
       61 ggcggccagc cctcgccagc cttcagggcc gagatagagc gcaacatcac cgagctcgat
      121 caactggtgg acgagatttt gctggccagc cggcttgacg ccggcgccgc cgacgtgggc
      181 acggtggagc ccgtcgatct ggtgggcctg gccgtggagg aatgcgtgcg cgtggacgcc
      241 gagctacagg tgggcgcagg cggcgcggat gacgatggcg ccgccaccat cgaggtgccc
      301 ggcgtctcca agctgctgcg ccgcgccgtg cgcaacctgc tggagaacgc gcgccgctac
      361 agccagggcg agatcacgct gcacatcacg cgcgatggca gcaccgccgt gctgcgcgtc
      421 tgcgacctcg gcccgggcgt gccacccgac cagcgcgaac gcatcttcga gcccttctac
      481 cgcctgcccg gcgccagcga gcgcgccggc ggcgtgggcc tgggcctggc cctggtgcgc
      541 tccatcgccg cacgccatgg cggcagcgtg gtctgtgagg ggcggccgga tggcgctgcg
      601 ggtgcatgct ttgtgctgcg gctgccgacc accaattgac cagctcggcc agttcagtag
      661 gtactcatac ccaacgtcag ccggacaact ccctggccat caacatgacg cgggacaact
      721 gtcatcacag gcagattgca aacaactgtc gatttttttt acaccaaccc cgccacagaa
      781 cttacgacac atcacaacac tatgaattta ttggcttttt catgtttggc acaaattatg
      841 cttattgcgc ttggcaacaa ccatggagaa gatcatgaag gcatttcaac tgatgggttt
      901 ggcgatcggc gccctgctag gtgccggcac agccgcagcc ggcccgctgc caggcggctg
      961 gtcctgcact ggcaactgcg gcaccaacac cgccgctgac ggcgacgtaa cgttggcgcc
     1021 aggcttcggc tcttatcaat ggatttcgac ggcgggcggc ctaacgggtg ccggccagct
     1081 gccagtcggc cccaccggcc aggagaccaa tggctcgtcg gcgcaaacgc ccactttctc
     1141 ggtgaacgct ggcgacaagc tgaacttcta tttcaactac atcacctctg acggcgtgca
     1201 attcaccgaa tacgcctggg ccggcctgta ttcaggcacc agcactttcg attcgtattt
     1261 gttcaccgca cgcaccacaa ccagcggcaa caccgtgcca gggttcggac tgccaggcct
     1321 tggcgccggc gtgacgctga cgccctccac ttcggccatc atccccggcg gccccgactt
     1381 ttctcccctg ggcggcagct ctggtgggtg ctacggaggt tccggacagg gctgcggcta
     1441 caccggctgg atccagatgg agtacacatt caccaccgcc ggcatctatt cgctgggctt
     1501 cggcgtcacc aatgcactcg acacggcatt cgactccgcc tttgccatcg ctggcgtgtc
     1561 gatcaacgac acacccatcg accccaaccc ggtgcccgaa ccggcaaccc tgacgctgct
     1621 gggcgccagc ctgctgggcc tgggcgcatt gcgccgccgg cgccagcagg gctgacaagc
     1681 cccctgggca ccgcagcctc ggggcggtgc ccggtttgca tgcctttgca tgcaccgtct
     1741 ttggcgcccg gcagcgccag cccgcaaggc gcggtttcat gttccgtgtc acgccgggca
     1801 ccaacttcct cgccagcaaa ctggccaagt ggagccaggt ggtcaagacc gcgaaaatgg
     1861 cggtgcaccg aggccccaaa ggaagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnggct ttctggaaca aggcagccgt ttcttgaacc ccgttttcgt gggcgatacc
     2041 ctgtactgca ccctcaccgt gtcggacctc aagccgggca acaccacggg cgtgatcacc
     2101 ctcggcaccg aggtgaaaaa ccagaagggt gtggtggtga tggatggctt tcaaaaatat
     2161 ctgctgagaa aacgccatcc gggtcagtga accacgcggg ccttgcagcg ctgcctgcca
     2221 tggcacatcc ccagcgcccg tgctgccatt cacgcgggtg cggtgcgccc atctgctaca
     2281 acccgtggta tcgcaatgcc tcggagaaaa aaccatgagc acagccgaca tcggcaccct
     2341 gcagcgcacg ccgacgggct ttgaaggcca actgacgcgc cagctcgacc acgacgcagt
     2401 agccgtctgg cgcatgctga ccgacccgct ggccatggcg caatggctgg cgccgggcag
     2461 cgtggagcag cgcgccggcg gcaccgtgca catcgacttt gccgacagcg gcaccgtcat
     2521 cgacagccgc gtcagcgcct ttgaggatgg tcgcctgctg gcctattcgt ggagcagcgg
     2581 cagcgagccc gaacgcccca tgcgctggga gctggtgccc gcgaatggag gcacgcggct
     2641 cacgctgacg ctgcacattc ccgcccagga ggacgccgcc aaggcctgcg ccggcttcga
     2701 gggccatctg gacatgctgg ccgcagccct ggagggcgtg cccatcaagt tcccgttcca
     2761 gcgctttctg caagcgcgcg gcgcctatac cgagcagctg gcgggttgag catcgccatg
     2821 accagcagcg cctaccaccc cgccgcaccg ccgctgcagg tggcgcaatg gttcaacacg
     2881 cgcgagccgc tctcgctcga atcgctgcgc ggacgcgtgg tgctgctgca cgcctttcag
     2941 atgctgtgcc cgggctgcgt ggagcatggc attccgcagg cgcggcgcgt gcacgaggcg
     3001 ttttcgccgg atcaggtgac ggtcatcggc ctgcactcgg tattcgagca ccacgccgtg
     3061 cagggcacgc cggcggcgct gcaggccttc atccacgaat accggctgcg ctttccgata
     3121 gcgctggatc agccccaccc gacgcgcaat attccgctga ccatgcaaga gctgatgctg
     3181 cagggcacgc ccagcgtggt ggtcatcgac gcccagggcc gcgtgcgcct gcaccacttc
     3241 ggccatctgg acgatctgcg cctgggcgcg ctgctgggcc agctggtggg tgaagccacg
     3301 gcgcctgtgg caagcgcgcc agcggcgcct ggctgcgacg accacggttg cgcccgcccg
     3361 gatctgccac agtcatgaac agcaccccgc cacaagcggt ggccaagttg cgcgccctgc
     3421 tcaccggccg cgccctgccc tacacgcggc ccggcagcgt gagcgccatc gccaagcagc
     3481 cgcgcagcgg cccggtggcc gtgggccttc tcgggcttgc cgacgacgag cagggcgatc
     3541 cgcgcgtgca tggcggcccc gacaaggccg tgcactgcta cgccttcagc caatacgccc
     3601 cctggcgcca ggagctggcg ggcaacgcca cggcccaggc gctgctagcc cagcccggcg
     3661 cctttggcga gaacttcagc ctggacggta tcaatgagtc tcaggtctgc ctggccgacc
     3721 agtggcaggt aggcagcgcg cgcttcgagg tcagccaggg gcgccagccc tgctggaagc
     3781 tcaatgaccg ttttggcctg cccgacatgg cgctgcgcgt gcagaccagc ctgcgcgccg
     3841 gttggtacct gcgcgtgctg caggttggac aggtgcaggc cggcgatacg gtgtggctgc
     3901 tggcgcggcc gcaccccgaa tggcccatag accggctgct gcgcgtgatt gccgagcgcg
     3961 actgcgcccc cgagacgctg cgcgagatac tgacactgcc gctgccgccg tcctggcacc
     4021 ggctgttcac gcgccggctg gagagcgcca gcgtcgaaga ctggagctcg cgcctggtcg
     4081 gcaagccctg aacctcaacg caccagcggc cagcgccaga ccgacgcaag atacatcagc
     4141 gcgcccgcca ccagggccgc cgccgccagc gccagcccct gggcaaagcc ctgcgcctgg
     4201 ccaccacgcg ccagcaacca ggccaccatg ggtgggccca tgatctgccc caggccatag
     4261 acggcggtga tcaggctggc aaagctgtcg gccgacgccg gccacaggcg ccgcgcttcc
     4321 tgcagcccat agaaggtgat ggccgtgaac ggcaggccca gcaggatgct gctgagtgca
     4381 aagcccgccg gtgtgggcca ccacaggcct atgccgatgc cgccggcctg catggcgtag
     4441 gccgccacca gcaaccagcg cctgtcccag cgcgtcgggg cgcgtgtgct cagcgccgcg
     4501 cccacggcta cgccggcgcc gaacatgggc cagaacaaat ccggccaggg cgaacccgcc
     4561 ggcagcgcgc cgcgggcgat caccggcaga aaggtggcgg tgacgatgta gcccagcccc
     4621 gccagcccat aggccagcgc atgcacgcca cgcgccgccg ccgcgccgcc gcccgcggc
//
LOCUS       DAOMTQ010000195         4617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4867_length_4617_cov_23.318281, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000195 DAOMTQ010000000
VERSION     DAOMTQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4867_length_4617_cov_23.318281"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2728)
                     /locus_tag="PK518_14150"
     CDS             complement(<1..2728)
                     /locus_tag="PK518_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50099.1"
                     /translation="MTTPTDFIAQWGPGGSSAHLNEKQGAQSYFIALCELLDVPKPDS
                     PEVAQEYSFERQSLILGEARGYADVFYRDHFAWENKAPGKNLDAALRQLQQYSLALSN
                     PPLLVVCDRLTIRIHTQFNGHPSETHTVRIEQLDQPEKRQLLRRLWTDPQSFRPRQTN
                     RDITEAAAKSFALLAEQLRGRSHHPDAVAHFLTQCLFCFFAEDVGLLPGRMFERLVGN
                     RQITSERLTTGLAQLFTTMQTGGLYGPDDIPWFNGGLFAHIHVPKLEILDITELRNAA
                     GLNWSAIDVSIFGTLFERGLDPAKRSQLGAHYTDPATIARIIEPVITRPLLQEWELLA
                     QDLQAQTAKIRKHGDAAYKKAHNRFVQWLERLRAFRILDPACGSGNFLFLGLKALKDV
                     ELYCITQAANLGLAREQDLVTGPHNMLGIELNEYAAELARVSLWIGEIQWRIAHGYEV
                     KTNPVLEPLEQIECRDALLSWLPRPQAREGIPPNLPLPLAGEGRGEGDSTGRTAEAPP
                     HPCPLPQGGEGATAATAAAPALAPAHPATQASGSDPDFAQRNSGSDPEAGPPGATATE
                     APWPRADVVVGNPPFLGGSKKRGELGGNYFDALNLVYGSRVPGFADLVCYWFDKALQE
                     IQLHGLQAAGLVATNSIRGGVNRTVLEAICKDSAIFDAWSDEPWVNDGAAVRVSLVCF
                     GNCNATRLNGRTTTHIDANLADLAGFDASTAVHLPENENTAFEGTKKYGTFEISGEIA
                     RYWLRLPNVNGMSNSRVIRPWSNGANVVRRPQDMWIVDFGVNATEPEASLYEAPFEHV
                     LKHVKPTRKDAKWWLHERSRPAMRIALERLARFALTVRVSKHRLFVWSPGSAIPDTAT
                     VAIARADDTTFGILHSRMHELWSLRMGTWLGVGNDPRYTPTTCFETFPFPAGL"
     gene            complement(2776..3069)
                     /locus_tag="PK518_14155"
     CDS             complement(2776..3069)
                     /locus_tag="PK518_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50100.1"
                     /translation="MSHNTALRQLDELQAQLHALRAQQTSNHAFSAGARGHAALLAAL
                     PPRYAEVLLALLDRLEAGALFTEESCSFSHQGLLDSLQQWLDKARATLAGKTA"
     gene            3137..4417
                     /locus_tag="PK518_14160"
     CDS             3137..4417
                     /locus_tag="PK518_14160"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRM50101.1"
                     /translation="MNALNIAEYTHTLGLQAKTASALMARAPAAIKNKALLALARLLR
                     ENVQALQADNAADLARARAAGLPEPMVDRLKLTPAVLETCALGCEQLAAMPDIIGEII
                     GMKQQPSGIRVGQMRVPIGVFGMIYESRPNVTIEAASLSIKSGNACILRGGSEAIDSN
                     RALARLVALALAEAGLPEHGVQLVQTTDRAAVGQLIAMPAYVDVIIPRGGKGLIERIS
                     AEAKVPVIKHLDGNCHSYVDDPCDLDLAVTVVDNAKTQKYSPCNATESLLVARAVAPA
                     FLPRIGAVFAEKGVEMRVCAESRALLATVSGAKLVDATEADWSEEYLAPIISIRVVAG
                     LDEAIAWINRYSSHHTEAILTTDHRHAQRFLREVDSASCMVNASTRFADGFEYGLGAE
                     IGISTDKFHARGPVGLEGLTSLKYVVLGEGEVRR"
BASE COUNT          728 a         1363 c         1673 g          853 t
ORIGIN      
        1 tcaggccggc ggggaagggg aaggtttcga agcaggtggt gggggtgtag cgcgggtcgt
       61 tgcccacgcc tagccaggtg cccatgcgca gcgaccagag ttcgtgcatg cggctgtgca
      121 ggatgccgaa ggtggtgtcg tcggcgcggg cgatggcgac ggtggcggtg tcggggatgg
      181 cgctgcccgg gctccacacg aagagtcggt gtttggatac acggacggtg agcgcaaaac
      241 gtgccaagcg ctccagcgcg atgcgcattg cggggcgact gcgctcatgt aaccaccact
      301 tggcatcctt gcgtgtgggc tttacatgct tcaagacgtg ttcgaacgga gcctcgtaga
      361 gcgatgcttc gggttcggtg gcgtttacac cgaaatcgac aatccacatg tcttgcgggc
      421 ggcgcacaac gtttgcgcca ttactccacg ggcggattac ccggctgttg ctcatgccgt
      481 tgacgttagg caatcgcagc caatacctgg cgatctcacc cgagatctca aaggtgccgt
      541 atttcttggt gccctcgaat gctgtgtttt cgttttccgg caaatgaacg gcggtactgg
      601 catcgaagcc ggcaaggtca gccaaattgg catcgatgtg cgtggtcgtg cggccgttaa
      661 ggcgtgtggc gttgcaattg ccaaaacaga ccaatgacac tcgcactgct gccccgtcgt
      721 tgacccacgg ctcgtcagac caagcatcaa aaattgcaga gtctttgcat atggcctcca
      781 ggacggtgcg gttgacgccg ccgcgaatgg agttggttgc gaccagccct gccgcttgta
      841 aaccgtgcaa ctgaatttct tgcagggctt tgtcgaacca atagcacacc aagtcagcaa
      901 acccggggac gcggctgcca tagacgaggt tgagagcatc aaagtagttg cctcccagct
      961 cgccgcgttt cttgctgccc cccaaaaacg gcggattgcc caccaccacg tcggcgcggg
     1021 gccagggggc ttcggtggcg gtagcgcccg gtgggccggc ttcggggtcg gagcccgagt
     1081 ttcgctgcgc gaaatcggga tccgaccccg aagcctgggt ggctgggtgc gcgggggcta
     1141 gcgctggcgc ggcggcggtt gcggctgttg ctccctcgcc cccttggggg agagggcagg
     1201 ggtgaggggg tgcttcggcg gtgcgcccgg tggagtcacc ctcacccctg ccctctcccg
     1261 caagcgggag agggagattg ggggggatgc cctctctcgc ttgcgggcga gggagccagg
     1321 acagcagcgc gtcgcggcat tcgatctgct ccagcggctc cagcacgggg ttggtcttga
     1381 cctcgtagcc gtgggcgatg cgccactgga tctcgcctat ccacagcgac acgcgcgcca
     1441 gctcggcggc gtattcgttc agctctatgc ccagcatgtt gtgcgggccg gtgaccagat
     1501 cctgctcgcg cgccaggccc aggttggcgg cctgggtgat gcagtacagc tccacgtcct
     1561 tcagcgcctt caggcccagg aacagaaagt tgccgctgcc gcaggcgggg tcgaggatgc
     1621 ggaacgcgcg cagccgctcc agccattgca cgaagcggtt gtgggccttt ttgtaggcgg
     1681 cgtcgccatg tttcctgatc ttggccgttt gcgcttgcag gtcttgcgcc agaagctccc
     1741 attcttgtag caatggccgg gtgatgacgg gctcgatgat gcgggcaatg gtcgccgggt
     1801 cggtgtagtg cgcgcccagc tggctgcgct tggcgggatc caggccgcgt tcgaacaggg
     1861 tgccgaagat ggagacgtcg atggcgctcc agttcaggcc ggcggcgttg cgcaactcgg
     1921 tgatgtcgag gatctccagc ttcgggacgt ggatgtgcgc gaacagcccg ccgttgaacc
     1981 aggggatgtc gtccggcccg tacaggccgc cggtctgcat ggtggtgaag agctgcgcca
     2041 ggccggtggt caggcgctcg ctggtgatct ggcggttgcc caccaggcgc tcgaacatgc
     2101 gcccgggcag caggcccacg tcttcggcaa agaagcagaa caggcattgg gtgaggaaat
     2161 gcgccaccgc gtcggggtgg tggctgcgcc cgcgcagctg ctcggccagc agggcaaagc
     2221 ttttggcggc ggcctcggtg atgtcgcggt tggtctggcg cgggcgaaag ctttgcgggt
     2281 cggtccacag ccggcgcagc agctggcgct tctccggctg gtcgagctgc tcgatgcgca
     2341 cggtgtgcgt ctcgctcggg tggccgttga actgggtgtg gatgcggatg gtcaggcggt
     2401 cgcagaccac cagcagcggc gggttggaga gtgccaggct gtactgctgc agctggcgca
     2461 gcgcggcgtc caggttcttg ccgggggcct tgttctccca ggcgaaatgg tcgcggtaga
     2521 acacgtcggc atagccgcgc gcctcgccca ggatgaggga ctggcgctcg aagctgtatt
     2581 cctgcgccac ctcggggctg tcgggcttgg gcacatcgag cagctcgcac agggcgatga
     2641 agtagctttg cgcgccctgc ttttcgttca ggtgggcgct gctgccgccg gggccccatt
     2701 gggcgatgaa gtcggtgggg gtggtcacgg gttgcttttg ttttgctagc tgctggcgct
     2761 ggtttggcgg gcgtatcagg cggttttgcc cgcaagtgtg gcacgcgcct tgtccagcca
     2821 ttgctgcagg ctgtccagca ggccctggtg gctgaaggag cagctttcct cggtgaacag
     2881 ggcgccggcc tccaggcggt cgagcagggc cagcagcacc tcggcgtagc gcggcggcag
     2941 ggcggccagc agggcggcgt ggccgcgcgc gccggcgctg aaggcgtggt tgctggtctg
     3001 ctgcgcgcgc agggcgtgca gctgggcttg cagctcgtcg agctggcgca gggcggtgtt
     3061 gtggctcatg gggcgtagcg tagcgcctgt gccctggcgg gcgcgtggcg tggtgatctg
     3121 cgtcagaatc gcacccatga atgccctgaa catcgccgaa tacacgcaca ccctgggttt
     3181 acaggcaaaa acggcttccg cgcttatggc gcgggcgcca gcagctatta aaaacaaagc
     3241 attgctggcg ctggcgcggc tgttgcgcga gaacgttcag gccctgcagg cggacaacgc
     3301 cgccgacctt gcgcgcgccc gagccgccgg gttgcctgag cccatggtcg atcgcctgaa
     3361 gctgaccccc gcggtgctgg agacctgcgc cctgggctgc gagcagctgg cggccatgcc
     3421 ggacatcatt ggcgagatca tcgggatgaa gcagcagccc agcggcatcc gcgtgggcca
     3481 gatgcgcgtg ccgattggcg tgttcggcat gatttacgag agtcggccga atgtgacgat
     3541 cgaggcggcc agcctgtcga tcaagagcgg caacgcctgc attctgcgcg gcggatcgga
     3601 agcgattgac tccaaccgcg cgctggcgcg gctggtggcg ctggcgctgg ccgaggccgg
     3661 cctgcccgag cacggtgtgc agctggtgca gaccacagac cgcgccgccg tgggccagct
     3721 gatcgccatg cccgcgtatg tggacgtgat cattccgcgc ggcggcaagg ggctgataga
     3781 gcgcatcagc gcggaggcca aggtgccggt gatcaagcac ctggacggca attgccacag
     3841 ctatgtcgat gacccttgcg acctggatct ggccgtgacg gtggtggaca acgccaagac
     3901 gcagaaatac agcccctgca acgccaccga gagcctgctg gtggcgcgcg ccgtggcgcc
     3961 ggccttttta ccgcgcatcg gcgccgtctt cgccgaaaaa ggggtggaaa tgcgggtttg
     4021 cgctgaaagt agggcgctgc tagctactgt ttcaggagca aaactggtcg atgccaccga
     4081 ggctgactgg agcgaggagt atctggcgcc catcatctct atccgcgtgg tggcggggct
     4141 ggacgaggcg attgcctgga tcaaccgcta cagcagccac cacaccgagg ccattctgac
     4201 caccgaccac cggcatgcgc agcgcttttt gcgcgaggtg gattcggcca gctgcatggt
     4261 caacgccagc acgcgctttg ccgatggttt tgagtacggt ctgggcgcgg agatcggcat
     4321 cagcaccgat aaattccacg cgcgcgggcc ggtggggctg gaggggctga cgtcgctgaa
     4381 gtatgtggtg ctgggcgagg gcgaggtgcg gcgctgagcc gctgggccat gcgcttgttt
     4441 ttggatagcg cgttgcgccg tatccgcaag gctggggccc ggcttctccc tcgccccctt
     4501 gggggagagg gtaggggtga gggggttgtt caggcgcagt ggctgttgac atacagccct
     4561 cacccccgcc ctctcccgcg cgcgggagag ggagtcgaga cgccgcgcgc tggcgac
//
LOCUS       DAOMTQ010000196         4590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4909_length_4590_cov_27.093275, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000196 DAOMTQ010000000
VERSION     DAOMTQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4909_length_4590_cov_27.093275"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PK518_14165"
     CDS             complement(<1..785)
                     /locus_tag="PK518_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRM50102.1"
                     /translation="MGPLLDSFWRAVGYCLMPRIIALSLLPLILITLVAAGGAHFYWA
                     AAVAWTQAQLEGANWLQLLWGWLHHFGVDNLPVMLAPLLVLMLAIPIIVIVCVLVVAV
                     LMAPSIVSLVAQRRFEQLERKRGGTLFISVLWSLGSTLAAILALVLSMPLWLVPPLVL
                     VLPPLIWGWLTYRVMAFDALAEHASKPERQEIFKRHRLSLLAIGILTGLLGAAPGIVW
                     ASGVVFAAAFFVLVPVAIWIYTLVFAFSSLWFTHYCLAALQRLR"
     gene            complement(813..1817)
                     /locus_tag="PK518_14170"
     CDS             complement(813..1817)
                     /locus_tag="PK518_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRM50103.1"
                     /translation="MANALSWPSQWFDTAQQWLFEALVQPLLFALGQASVLEDAYAAT
                     GWLLVGLLQLAVMLVVIAPLERWRPVEPLRDHAAVRTDVIYTLIHRLGLVRVVLFFTT
                     AVLWDGLFGALRTHGWQTWQLDALWPGVTDQPLLSLLLYLLVFDLLDYWIHRGQHHFD
                     WWWSLHSLHHSQRQMTQWSDSRNHLLDDLLRDAIIVSVAQCIGVAPGQFVAIVALTQL
                     SENLHHANLRLWFGHWGERLWISPRFHRLHHAVGVGHESLAPAGPGTGSMALGGRNFG
                     VLLPWWDMLFGTANFEHRYEPTGVRDQLEPGADGRLRDYGRGFWAQQWRGLLRLAGRG
                     "
     gene            complement(1828..2685)
                     /locus_tag="PK518_14175"
     CDS             complement(1828..2685)
                     /locus_tag="PK518_14175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM50104.1"
                     /translation="MPNADRHSPSSRRVYIHFAIYVIAACALPISAKAENACKGTVYL
                     TFDTGHMGVASLVAEVLHRQGVQATFFAADEHTQTGGSSLDEHWAPWWRARVAEGHEF
                     ASHTMHHVYWRGDAGTARQPSFKVRPTAGPQAGQNLTLTAAQYCAQIDAATQRLRTIT
                     GKEPLPMFRAPGGKTSPRLLRVAQSCGYHHVGWTPAGFLGDELPSERYSNAQLLQQAL
                     RAIHDGDVLLAHLGIWSRKDPWAPAVLEPLIQGLKERGLCFRTLREHPRFQAGLTSDD
                     AARSGSGRR"
     gene            complement(2672..3619)
                     /locus_tag="PK518_14180"
     CDS             complement(2672..3619)
                     /locus_tag="PK518_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HRM50105.1"
                     /translation="MFMVACWACGLQAAQPPVFVLNSLQADISVIDPESWVETARIPT
                     GKEPHHLYLTPDEKSLIVANALGDSLTFVDPATAQVQRTVRGIVDPYHLRFSPDMHWL
                     VTAANRLNHVDFYRWDGQNLTLVQRVPTGKTPSHLWIDSGSRVLYASMQDSDELVAID
                     IGTQKVLWRTSTGAMPADVYGSPDGKRIFVGLTGSDSVEVFDISGEQPRSVQRIKTGS
                     GAHAFRAAGDGRHLFVSNRVANTISKVDMQALRVVDSYPAPGGPDCMDVSADGRWIYV
                     SARWARKLLVIDTVERKVARSIPVGKSPHGVWTLKHAKR"
     gene            complement(3782..4519)
                     /locus_tag="PK518_14185"
     CDS             complement(3782..4519)
                     /locus_tag="PK518_14185"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HRM50106.1"
                     /translation="MQLQDILYSQGFGTRRVCAGLIQQGWVSVYQANLPEPMTCLEAT
                     QDFQAQDLVLRVQGFDWPYQAKAYVMLHKPVGTECSQKPSAYPSVYTLLPAPLRQRPS
                     KGAVSGVQAVGRLDQDTTGLLLLSDDGQFIHRMSSPKKHVPKVYQVTTKHPVTAAQIE
                     RLQAGVVLDDDPRPVRAAACVQLDERCIDLTLTEGKYHQVKRMIAAVGNRVEALHRSR
                     IGGLSLPDDLQPGQWRWLTAQQIALLT"
BASE COUNT          966 a         1500 c         1374 g          750 t
ORIGIN      
        1 cgcagccgct gaagcgctgc caggcaatag tgcgtgaacc acagcgagga aaaggcaaat
       61 accagcgtgt agatccagat cgccaccggc accagcacaa agaaagccgc cgcgaacacc
      121 acccccgagg cccagacgat gcctggcgcg gcgcccaaca gcccggtcag aatgcctatg
      181 gccagcaggc tgaggcggtg gcgcttgaag atctcctgcc gctctggctt gctggcatgc
      241 tcggccagcg catcaaaggc catcacccga taggtcagcc agccccagat cagcggcggc
      301 agcaccagca ccaggggcgg aaccaaccac agcggcatgg aaagcaccaa ggccaggatc
      361 gccgccaggg tggagccaag cgaccacagc acgctgatga acaaggtgcc gccgcgcttg
      421 cgctccagtt gttcgaagcg ccgctgcgca accaatgaca cgatggaagg cgccatcagc
      481 acggccacga ccagcacaca gacaatgacg atgatgggta tggccagcat gagcaccagc
      541 agcggcgcca gcatcacggg caggttgtcc acgccaaagt ggtgcagcca gccccacaat
      601 agctggagcc agttggcacc ctccagctgg gcctgtgtcc aggccacggc cgctgcccaa
      661 tagaaatgcg ccccgcctgc ggccaccagg gtgatcagga tcagtggcaa caacgatagc
      721 gcaatgatgc gcggcatcag gcaatagccc acggcgcgcc agaaagaatc taagagtggc
      781 cccatacccg caagcatagc gcggctgccc gctcagccac gccccgccag gcgcaacagg
      841 ccgcgccatt gctgcgccca aaagcctcgg ccgtagtcgc gcaagcggcc atcggccccc
      901 ggttcgagtt ggtcgcgcac ccccgtgggc tcataacgat gctcgaagtt ggccgtgcca
      961 aacagcatgt cccaccaggg cagcagcacg ccaaagttgc gcccgcccag ggccatgctg
     1021 cccgtgcccg gccctgcggg tgcaagcgat tcatggccca cgccgacggc atggtgcagc
     1081 cggtgaaaac gcgggctgat ccacaggcgc tcgccccagt ggccaaacca caggcgcagg
     1141 ttggcatggt gcaggttttc gctgagctgc gtcagcgcga cgatggccac gaactgcccc
     1201 ggcgccacgc ctatgcactg cgccacgctg acgatgatgg cgtcgcgcag caggtcgtcc
     1261 agcaggtgat tgcggctgtc gctccactgg gtcatctggc gctgtgaatg gtgcagggaa
     1321 tgcaggctcc accaccagtc gaaatggtgt tggccacgat gtatccagta atccagcaga
     1381 tcgaaaacca gcagatacag cagcaggctg agcaatggct ggtcggtcac accgggccat
     1441 agcgcgtcga gctgccaggt ttgccagcca tgcgtgcgca gggcaccaaa caaaccatcc
     1501 cagagcacgg cggtggtgaa aaacagcact acacgtacca ggcccagacg atggatcaag
     1561 gtgtagatca catcggtccg caccgccgca tgatcgcgca gcggctccac cgggcgccag
     1621 cgctccagcg gggcgatcac gaccagcatc accgccagtt gcagcaaacc cacgagcagc
     1681 cagccggtgg cggcgtacgc atcctccagc acgctggcct ggcccagcgc aaacagcagg
     1741 ggctgcacca gggcttcgaa caaccactgc tgagcggtgt cgaaccattg actcggccag
     1801 gacagggcgt ttgccatcaa tcggccctca tcgacgcccg gaaccactgc gggctgcgtc
     1861 atcagaggtc agcccggcct gaaagcgcgg atgctcacgc agggtacgaa aacacaggcc
     1921 acgctccttc aggccctgga tcaggggttc gagcacggcc ggcgcccagg gatccttgcg
     1981 cgaccagatg cccaggtgtg caagcagcac gtcgccatcg tgaatagcgc gcaaggcttg
     2041 ttgaagcagc tgggcgttgc tgtagcgctc gctcggcagc tcatcaccca ggaaaccggc
     2101 aggcgtccag cccacatggt ggtagccgca ggattgcgcc acgcgcaaca ggcgcggcga
     2161 ggtcttgcca cccggtgcac gaaacatcgg caacggctcc ttgccggtga tggtgcgcag
     2221 ccgctgcgtc gcggcatcga tctgtgcaca gtattgcgcc gccgtcaggg tcaggttctg
     2281 cccggcctgg gggccggcgg taggcctgac cttgaaggac ggctgccggg ccgtacccgc
     2341 gtcgccacgc cagtacacat ggtgcatcgt gtgcgaggcg aactcgtgcc cttcggccac
     2401 ccgcgcgcgc caccaggggg cccaatgctc atccaggctg ctgcctccgg tctgggtatg
     2461 ctcgtcagcg gcaaaaaaag tggcctgcac gccctgccgg tgcagcactt cggccaccaa
     2521 cgaggctacg cccatatggc cggtgtcaaa ggtcaggtag acagtgcctt tacaagcatt
     2581 ttccgccttt gcgcttattg gcaaagcgca agcagctatt acatatatag caaaatgaat
     2641 atagaccctg cgtgacgacg gcgaatggcg gtcagcgttt ggcatgtttc agcgtccaga
     2701 caccgtgggg cgacttgccc acggggatgg atcgtgccac cttgcgctcc acggtgtcga
     2761 tcacaagcag cttgcgcgcc cagcgcgcgc tcacgtagat ccagcgtcca tcggccgaaa
     2821 catccatgca atcgggccca cccggcgcag ggtaagagtc gacaacgcgc aatgcctgca
     2881 tatcgacctt gctgatggtg ttggccaccc ggttgctgac gaacagatgc cgtccgtcgc
     2941 cggcggcgcg aaaggcgtga gcgccactgc cggttttgat gcgctgcacg ctgcgtggct
     3001 gctcgcccga tatatcgaac acctccacgc tgtcgctacc ggtgagcccg acaaatatgc
     3061 gcttgccatc cgggctaccg tacacgtcgg cgggcatggc gccggtgctg gtgcgccaca
     3121 ggactttctg cgtaccgatg tcgatggcga ccagctcgtc actgtcctgc atggacgcat
     3181 acagtacacg gctgccgctg tctatccaca gatggctggg cgtcttgccc gtgggcacgc
     3241 gctgcaccag cgtcaggttc tggccgtccc aacggtaaaa atcgacatgg ttaagccggt
     3301 tggccgcagt caccagccaa tgcatgtcgg gcgagaaccg caggtgatag ggatccacaa
     3361 tgccacgcac cgtgcgctgc acctgggccg tggccggatc gacaaaggtc aggctgtcgc
     3421 cgagagcatt ggcaacgatg agcgattttt catccggcgt caggtaaaga tggtgcggct
     3481 ccttgccggt ggggatgcgc gccgtctcga cccaactctc cgggtcgatc acgctgatgt
     3541 cggcctgcag ggaattgagc acaaacaccg gcggctgtgc tgcctgcagc ccgcatgccc
     3601 agcaggcgac catgaacatc ccgacagccc gcagcatcat ccgttgtttc actcttgctc
     3661 ccttgaaatt agcgagtgcc aacaaccagc tgcagcacgc acgcccgatg aaaggcctga
     3721 tgcataacgc gcattcagta ttcatcgttg acgcgctccc aatcattgct gggaacgccg
     3781 actatgtgag cagcgcgatc tgctgcgcag tcagccaccg ccactgccct ggctgcaagt
     3841 cgtcaggcaa tgacaagcca ccgatacggg aacgatgcag tgcctcgacg cgattgccca
     3901 cggcggcaat catgcgcttg acctggtggt acttgccctc tgtcagtgtc aagtcaatgc
     3961 accgctcatc cagctgcacg caggcagcag cccgtaccgg gcgcgggtcg tcatccagta
     4021 ccactccagc ctgtaaacgt tcaatctgcg cggcggtcac tggatgtttg gtcgtcacct
     4081 gatacacctt ggggacatgc ttcttgggtg aactcatgcg atgaatgaac tgtccatcat
     4141 cactgagcaa cagcaagccg gtcgtatcct gatccagccg cccaacagcc tgcacaccac
     4201 tcaccgcgcc cttgctcggc cgctgccgca gtggcgccgg cagtagcgta tacacactgg
     4261 gataggctga tggcttttgt gaacattccg tgccgacggg tttatgcagc atcacatagg
     4321 ccttggcctg gtacggccag tcgaaaccct ggacacgcag caccaaatcc tgcgcctgaa
     4381 agtcttgcgt cgcttccagg catgtcatcg gctccggaag gttcgcctga tacacggata
     4441 cccagccttg ttgaatcaaa cccgcacaaa cacggcgagt gccaaagccc tgtgaataaa
     4501 gaatatcttg aagttgcata gagctcaaat caaatcccca aagcaaaacg ccctcccact
     4561 atgtaagtgg gagggcggtt tgggctgtaa
//
LOCUS       DAOMTQ010000197         4543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_4980_length_4543_cov_27.793226, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000197 DAOMTQ010000000
VERSION     DAOMTQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4980_length_4543_cov_27.793226"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1035)
                     /gene="fliG"
                     /locus_tag="PK518_14190"
     CDS             complement(40..1035)
                     /gene="fliG"
                     /locus_tag="PK518_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRM50107.1"
                     /translation="MDEQGLNDAAIMLMSLGEEEAAEVFKHLSPKEVQKLGETIARMR
                     AVSKEKVDEVLTRFTNDAAAQSLLVSDTGNYVRSVLKRALGDDKAALLIDRILQGGDV
                     SGIESLKWMDPLSVAELLRNEHPQIVAAILVHLDPDHASGILGQFTERQRGEVMLRIA
                     TLEGIQPTALKDLNEVLFKALAGGDKVRKSSLGGVQAAASIINLMGSGMDAAVLESIR
                     GHDIDLAQKIMDKMFVFDDVAKLDDRSIQTVLREVASETLVVALKGAQPEVRDKILNN
                     MSSRAAAAMREDLESRGPMRLSEVEAQQREILKTVRRLADEGQIALSGGGDDAFV"
     gene            complement(1066..2760)
                     /gene="fliF"
                     /locus_tag="PK518_14195"
     CDS             complement(1066..2760)
                     /gene="fliF"
                     /locus_tag="PK518_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRM50108.1"
                     /translation="MSAVAEVPVTPPPAASAGWQQRLASLDRGARLRLGAAALLLLGV
                     IAAAIFYGRSPDYRVLFSGLNDKDGGAIVAQLTTMNVPYKYTEGGGAIMVPAERVHDV
                     RLRLATQGLPKGSVTGFELMETNRFGVTQFQERLNFQRGLEGELTRSIQALASVQSAR
                     VHLALPNQNGFFREQQKPSASVLLSLYPGRMLDRAQLAGIVHLVASSVPELAPSAVSV
                     LDDTGKLLSQSPDGGASNGVDAQQLAYVQQIEQQYTRRILDLLEPVVGKNNVKAQVTA
                     EVDFNQTESTVEQHRPNLAPDASAVRSQQIVESGGDKGAQPPTGVPGAVSNQPPQGST
                     APINGANPPPTAANAQQQPGAAGQGKRESITNYEVDKTTRVTRGGMGAIKRVNAAVVV
                     NYQSVSEEEGKPPVAKALSPEQIEQMTALVRETIGYSKERGDSVNLMNTPFLADAAPA
                     ADLPLWKQPEVVELAKSLGWPVGLSLAVALLLLGLVRPVLKGRKSATPAQDSAPGGQL
                     NALASEELERPALPAPEQPQDLQPTAEQLRLEDARRLARENPVAVASIIKTWVNGE"
     gene            2937..3248
                     /gene="fliE"
                     /locus_tag="PK518_14200"
     CDS             2937..3248
                     /gene="fliE"
                     /locus_tag="PK518_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRM50109.1"
                     /translation="MDLRINSTPNPLTSTQLARRVTAPQPGTQEVGFSSAFKNALQSV
                     SSAQDRASALQREVQLENPKVSIEETMVAMQKAQVGFQATLHVRNRMVQAYTDIMNMQ
                     V"
     gene            3466..3708
                     /locus_tag="PK518_14205"
     CDS             3466..3708
                     /locus_tag="PK518_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRM50110.1"
                     /translation="MFAERHVRLFRNGRNQAVRIPREFELDAQDAIMRREDGRLVIEP
                     VRKQGLLATLAALAPLAEDWPDDLDAGLQALDDIKL"
     gene            3705..4160
                     /locus_tag="PK518_14210"
     CDS             3705..4160
                     /locus_tag="PK518_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRM50111.1"
                     /translation="MSAPRYLLDTNVLSDLVRHPQGIVARKIAAVGEAAVCTSIIVAA
                     ELRFGAAKRGSARLSHQVHAILSAIEVLPLDMPADTTYAQLRWALEQSGCPIGPNDML
                     IAAQALAHECVLVTANVGEFSRITDLQVTDWLTVLDEPPPDTSPTTSTG"
     gene            complement(4274..>4543)
                     /locus_tag="PK518_14215"
     CDS             complement(4274..>4543)
                     /locus_tag="PK518_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HRM50112.1"
                     /translation="AIENSSAKMLEAARLKDWDEVVRYEGACAVLIEQLRYRAQNEDL
                     EPEQRKEKTRIMQRILRNDAQIRILAEPWLAQFDHVLEGQPQVLH"
BASE COUNT          714 a         1598 c         1441 g          790 t
ORIGIN      
        1 cccagctctt cgccggggat gaagcgcgag tagtggcggc tagacgaagg catcgtcacc
       61 tccgccgctc aatgcgatct ggccttcgtc ggccaggcgg cgcacggtct tcagaatctc
      121 gcgctgctgc gcctcgacct cggacaggcg catggggcca cgcgactcca ggtcttcgcg
      181 catggcagcg gccgcgcgcg aggacatgtt gttcaggatc ttgtcgcgca cctcgggctg
      241 ggcgcccttc agggccacca ccagggtctc ggaggccacc tcgcgcagca cggtctggat
      301 ggagcggtcg tccagcttgg ccacgtcgtc aaagacaaac atcttgtcca tgatcttctg
      361 cgccaggtcg atgtcgtggc cgcggatcga ctccagcacc gccgcatcca tgcccgagcc
      421 catcaggttg atgatgctgg ccgccgcctg cacgccgccc agcgagctct tgcgcacctt
      481 gtcgcccccg gccagggcct tgaacagcac ctcgttcaag tccttgagcg ccgtgggctg
      541 tatgccctcc agcgtggcga tgcgcagcat cacctcgccg cgctggcgct cggtgaactg
      601 ccccaggatg cccgaggcat ggtccggatc caggtgtacc aggatggcgg cgacgatctg
      661 cgggtgctcg ttgcgcagca gctcggccac cgacagcggg tccatccact tcaggctctc
      721 tatgcccgag acatcgccgc cctgcaatat gcggtcgatc aagagcgccg ccttgtcgtc
      781 gcccagcgcg cgcttgagca ccgagcgcac gtagttgccg gtgtcggaca ccagcaggct
      841 ttgcgccgct gcgtcgttgg tgaagcgggt cagcacctcg tccaccttct ccttggacac
      901 ggcgcgcatg cgggcgatgg tctcgcccag cttctgcacc tccttgggcg acaggtgctt
      961 gaagacctcc gccgcctcct cctcgcccag cgacatcagc atgatggctg cgtcgttcag
     1021 tccctgttcg tccatatcgg tctcgccgtc agctacgggc cggcgtcatt cgccgttgac
     1081 ccaggttttg atgatgctgg ccacggccac cgggttctcg cgcgccaggc ggcgggcgtc
     1141 ctccagccgc agctgttcgg ccgtgggctg caggtcctgc ggttgctcgg gggcgggcag
     1201 cgcggggcgc tccagctcct cggaggccag ggcattcagc tggccgccgg gcgccgaatc
     1261 ctgcgccggc gtggcgctct tgcggccctt gagcaccggg cgcaccagcc ccagcagcaa
     1321 cagcgccacg gccagcgaca ggcccacggg ccagcccagg ctcttggcca gctcaaccac
     1381 ctcgggctgc ttccacagcg gcaggtcggc ggcgggcgcg gcatcggcca ggaagggcgt
     1441 gttcatcagg ttcaccgaat cgccgcgctc cttgctgtag ccaatggtct cgcgcaccag
     1501 ggccgtcatc tgctcgatct gctcggggct cagggccttg gcgaccggcg gcttgccctc
     1561 ctcctcgctc acgctctggt agttgacgac cacggccgcg ttcacgcgct tgatggcacc
     1621 cataccgccg cgcgtcacgc gcgtggtctt gtccacctca tagttggtga tggattcgcg
     1681 cttgccctgc cccgccgcgc ccggctgctg ctgggcattg gcggccgtgg gcggcgggtt
     1741 ggcgccgttg atgggcgccg tggagccctg cggcggctgg ttgctgacgg cgccgggcac
     1801 gcccgtgggc ggctgcgcgc ccttgtcgcc gccgctttcc acgatctgct ggctgcgcac
     1861 ggcgctggcg tcgggtgcca ggttggggcg gtgctgctcc accgtggact cggtctggtt
     1921 gaagtccacc tcggccgtca cctgggcctt gacgttgttc ttgcccacca cgggctccag
     1981 caagtccagg atgcggcgcg tgtactgctg ctctatctgc tgcacatagg ccagctgctg
     2041 cgcgtccacg ccattgctgg cgccgccgtc gggcgactgc gacagcagct tgccggtatc
     2101 gtccagcacg ctcaccgccg acggcgccag ctcgggcacg ctggatgcca ccaggtgcac
     2161 gatgcccgcc agctgcgccc gatccagcat gcggcccggg tacagcgaca gcagcaccga
     2221 cgccgaaggt ttttgctgct cgcgaaagaa accgttctga ttgggcagcg ccaggtgcac
     2281 gcgcgcgctc tgcaccgagg ccagggcctg gatggagcgc gtcagctcgc cctccagccc
     2341 acgctggaaa ttcagccgct cctggaactg ggtcacgcca aagcggttgg tctccatcag
     2401 ctcgaagccc gtcaccgagc ccttgggcag gccctgcgtg gccaggcgca ggcgcacgtc
     2461 gtgcacacgc tcggccggca ccatgatggc gccgccgcct tcggtgtatt tgtagggcac
     2521 gttcatggtg gtcagctggg ccacgatggc gccgccatcc ttgtcgttca ggcccgagaa
     2581 cagcacccgg tagtccgggc tgcggccata gaagatcgcc gcggcgatca cccccagcag
     2641 caacaacgcc gccgcaccca ggcgcaggcg cgcaccgcgg tcgagcgaag ccaggcgctg
     2701 ctgccagccg gcgctggcgg cggggggcgg ggtaacaggg acttcggcaa ctgcagacat
     2761 cgtgctttcc acaaaagggc gggcgccagg cgggactctg gccgtgctgc gattattgaa
     2821 gtcggccgcg atgccgttcg ctggataagc ccgccctttg gccatctttt cgcacggccg
     2881 cggctcgggt cgcgccctag gattgcacca tcgccccaac cctcgaccga ccggccatgg
     2941 acctgcgcat caacagcacc ccgaacccgc tgaccagcac ccagctggcg cgccgcgtca
     3001 cggcgccgca gcccggcacg caagaggtgg gcttttcatc ggctttcaag aacgccctgc
     3061 agtcagtcag cagcgcgcaa gaccgcgcca gcgccctgca gcgcgaggtg cagctggaaa
     3121 accccaaggt cagcatcgaa gagaccatgg tcgccatgca gaaggcacag gtcggctttc
     3181 aggccacgct gcatgtgcgc aaccgcatgg tgcaggccta tacggacatc atgaacatgc
     3241 aggtgtgacg ctattgcggc atcacattac tacttatttg atagcgcatg gcgccttatt
     3301 gacgggcgca acatccgttt tcatgcaaga cccctgtacc ggcgggctgg ggcgcgcggc
     3361 cctggagact atttcaaccg cgggcgcatg tcctgcccgg atttgacctg aagccactcc
     3421 aagcatatgc tttgacatat gccaattcaa atggaggaaa agaacatgtt tgccgaacgc
     3481 catgtccgcc tgtttcgcaa cgggcgcaat caggccgtgc gcatcccccg tgaattcgag
     3541 ctggacgcgc aagacgccat catgcgaaga gaagacggtc gcctggtgat cgaacccgtg
     3601 cgcaagcagg ggctgctggc cacgctggca gcccttgctc cactggcaga agattggccg
     3661 gatgacctgg atgccggctt gcaagcgctg gacgacatca agctatgagc gcgccgcgct
     3721 acttgctgga caccaacgtc ctctctgacc tggtgcggca cccgcaaggc atcgtggccc
     3781 gcaagattgc ggccgtcggc gaggccgccg tatgcacatc catcatcgtg gcggccgaac
     3841 tgcgctttgg cgccgccaag cgcggctccg cccggctctc gcaccaggtg cacgccattt
     3901 tgtcggccat cgaagtgctg ccgctggaca tgcccgccga tacaacctac gcgcaactgc
     3961 gctgggcact ggagcagtcg ggctgcccca tcggccccaa cgacatgctg atcgccgccc
     4021 aggccctggc ccatgaatgc gtgctggtca cggccaacgt gggcgagttt tcccgaatca
     4081 ccgatctgca ggtcaccgac tggctgactg tcctcgacga accgcctccc gacaccagtc
     4141 cgacgacctc aaccggttga acctaagccg gcacaccggc cgggagcaca aagaaaaaga
     4201 gccggccacc tcacggcgcc ggctcactgc cttggggtac cttgcacgcg gcgccaccac
     4261 gccgccgcac ccgtcaatgc agcacctgtg gctggccttc cagcacatgg tcaaactgcg
     4321 ccagccaggg ctcggccagg atgcggatct gcgcgtcgtt gcgcaatatg cgctgcatga
     4381 tgcgcgtctt ctccttgcgc tgctcgggct ccagatcctc gttctgcgcg cggtagcgca
     4441 gctgctctat cagcactgca caggcgccct cgtagcgcac tacctcgtcc cagtccttca
     4501 ggcgcgcggc ctccagcatc ttggcgctgc tgttttcgat ggc
//
LOCUS       DAOMTQ010000198         4498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5066_length_4498_cov_23.684898, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000198 DAOMTQ010000000
VERSION     DAOMTQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5066_length_4498_cov_23.684898"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK518_14220"
     CDS             complement(<1..289)
                     /locus_tag="PK518_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRM50113.1"
                     /translation="MSLSDRYAPSTGLSRNAVIALSVVTLHVAALWALQSGLMRKAAE
                     IIIPAEVLSEFLTPQPPAPPAPPTPPAPPPPKPAPPKPSPTPPKPRVAKPTP"
     gene            complement(413..667)
                     /locus_tag="PK518_14225"
     CDS             complement(413..667)
                     /locus_tag="PK518_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRM50114.1"
                     /translation="MIVCVCRRVSDREIARHARAGMSFDEIQFELGVATQCGRCEDCA
                     RDVVAQCCSSQPVAALYKEGLPGATGAGCCGQQGAACGCA"
     gene            complement(866..1156)
                     /locus_tag="PK518_14230"
     CDS             complement(866..1156)
                     /locus_tag="PK518_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRM50115.1"
                     /translation="MATAKTAPAAPAKKRTPNAAFMKPLTPSPALAAVVGATPLPRTE
                     IISKLWVYIKAHNLQDAANKRMINADAKLKEVFGKPQVSMFEMAGLIGKHVK"
     gene            1367..1867
                     /gene="rraA"
                     /locus_tag="PK518_14235"
     CDS             1367..1867
                     /gene="rraA"
                     /locus_tag="PK518_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HRM50116.1"
                     /translation="MTTSAAFSTCDMCDAHKGDDSGSFWVLPPVFQCFGGVAAFSGPV
                     STVKCFEDNTLVKAAVDSPGQGRVLVVDGGGSLRRALVGGNLAASAARNGWAGIVVNG
                     CVRDVAELNAEQVGIRALALMPLPTEKRGEGLAEVAVQIQGVWVRPGDWLYADADGIV
                     VSSQAL"
     gene            complement(1899..2690)
                     /locus_tag="PK518_14240"
     CDS             complement(1899..2690)
                     /locus_tag="PK518_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HRM50117.1"
                     /translation="MSNAHELVTLAADLERMLKLRTPPIGMKLLESAETLRQIPKLRR
                     PQAIHTMDQIVGQAARLGWTMGVQAADLVGEQCRAVVGLGGQDADWYSGQAMTGVWFA
                     TVEDSARHQAAMDVVPVGRYQALVVAPLQRWPDFGAGADEPDIALFYATPGAMIYFIN
                     GLQWSGYERFDWSVVGESSCADSWGRALKTGKPSLSIPCFAERRYGGVLDDEMLMALK
                     PADIAKSLAGMQALARNGLRYPFASYGIQQDARAGMGVSYPKKEA"
     gene            complement(2726..3640)
                     /locus_tag="PK518_14245"
     CDS             complement(2726..3640)
                     /locus_tag="PK518_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM50118.1"
                     /translation="MSLVGSYVALSKPLAAAFPVLLLAWMRFGIGALAMLTWLKKPAD
                     EPPLTPQTRGLLFLQSLLGNFLFTVCMIYGVSLTSATSAGVIMACIPACVAVMSWLFL
                     RERVARRTWVAVACAVIGIALFSLSKPEHPAQSGSALEPDNAIYLQWLGYALLLCASV
                     CEGAYSVIGKKLTGAMGPKRITSLINLWGFLLSMPFGLYLAWGFDFAAVPWSIWLLLL
                     FYALAACMWTVWLWMTGLRAVPAAQGGVFTVLLPVSAALVGVLVLGERFTPLQLLAFG
                     LALASVVLATLPSRLALRVGRGAARPDD"
     gene            4038..>4498
                     /locus_tag="PK518_14250"
     CDS             4038..>4498
                     /locus_tag="PK518_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRM50119.1"
                     /translation="MSNSLNSQLSREQQIAALEKDWATNPRWKGVKRGYSAADVVRLR
                     GSLQPEYTLAKRGAEKLWEKVNGGAKKGYVNAFGAITAGQAMQQAKAGLEAVYLSGWQ
                     VAADGNTSETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWGRGINPGDK"
BASE COUNT          842 a         1401 c         1509 g          746 t
ORIGIN      
        1 cgggtgtcgg cttggccacg cgcggcttgg gcggcgtcgg tgacggcttc gggggcgcgg
       61 gcttgggcgg gggcggcgca ggtggtgtcg gtggcgcggg cggggctggc ggctggggcg
      121 tgaggaattc gctcaagacc tcggccggga tgatgatctc cgccgccttg cgcatcaggc
      181 cggactgcaa ggcccatagc gccgccacat gcagggtcac gacggacagc gcaatcacgg
      241 cgttgcggct cagtccggtg gacggggcat agcgatctga aagcgacata aatggatagc
      301 tgcaggcgct tgcccagcaa gggctacagg cctaaaagac gtgaaactct gcgccggctt
      361 gtgcagggcg caccgggccg agcatataac gacagcccgc tggcaaacgg actcaggcgc
      421 agccgcatgc cgcgccttgc tggccgcagc agcccgcgcc agtggcgccc ggcaggcctt
      481 ccttgtacaa ggccgccacc ggttgcgagg agcagcactg cgccacgacg tcgcgtgcgc
      541 agtcttcgca gcgcccgcat tgcgtggcca cgccgagctc gaactggatc tcatcgaagc
      601 tcatgcccgc gcgcgcatgg cgtgcaatct cacggtcgga tacgcggcgg caaacacaga
      661 cgatcatggt gcaggctgtt ctggcaggct atgaaagcga tgggcggatt ataaatacga
      721 atccatcgca ttctcgatct gtatccctgc tgtgatgtcg gacaacacgc tggccgtgcg
      781 gggacgaaaa aaaaccgacc cattgcgagg tcggtttttt tgcagctcgg tcgccttggc
      841 gccgctgcgg cctggtgccc gggccttact tgacgtgctt gccgatcagg ccggccatct
      901 cgaacatcga gacctggggc ttgccgaaca cttccttgag cttggcgtcg gcgttgatca
      961 tgcgcttgtt ggccgcgtcc tgcaggttgt gggccttgat gtaaacccac agcttgctga
     1021 tgatttccgt gcgcggcagg ggcgtggcgc cgaccacggc agccagggcg gggctggggg
     1081 tcaggggctt catgaaagcg gcgttggggg tgcgcttctt tgccggggct gcgggggcgg
     1141 tcttagcagt tgccatgtcg tacggtcctc ttggaaatga ataagtattg ccagcgaaag
     1201 tctgctgctt tactggttcg cgcgatgcta ctgggaaaaa aacgccgctc caagggaaat
     1261 cgtttctttt ttgcctcgat ttgttgcgtg tcaccgccgg cggccacgcc cgataatgcg
     1321 cgcgcggccc gccgcagccc cttcatcatc agagagacct tgccccatga cgacctcagc
     1381 agccttcagt acctgtgaca tgtgcgacgc ccacaagggc gacgacagcg gcagcttctg
     1441 ggtgctgccg cccgtattcc aatgctttgg cggcgtggcg gccttcagcg ggccggtcag
     1501 caccgtgaaa tgcttcgagg acaacaccct ggtgaaggcc gcggtggaca gcccgggcca
     1561 gggccgcgtg ctggtggtgg acggcggcgg ctccctgcgc cgcgccctgg tgggcggcaa
     1621 cctggcggcc agcgcggcgc gcaacggctg ggccggcatc gtggtcaacg gctgcgtgcg
     1681 cgacgtggcc gagctcaatg ccgagcaggt gggcatacgc gcgctggcgc tgatgcccct
     1741 gcccaccgaa aagcgcggcg agggcctggc cgaggtggcg gtgcagatcc agggtgtgtg
     1801 ggtgcgcccg ggcgactggc tgtatgccga cgccgacggc atcgtggtca gctcgcaggc
     1861 gttgtgaaac ccgttggcag tccgccggcc agcagggttc aggcctcttt cttcgggtag
     1921 ctcacgccca tgccggccct ggcgtcctgc tggatgccat aactggcaaa cgggtagcgc
     1981 aggccgttcc tggccagcgc ctgcatgccg gcgagtgact tggcgatatc ggcaggcttg
     2041 agcgccatca gcatctcgtc gtccagcacg ccgccatagc ggcgctcggc aaagcagggg
     2101 atggacaggc tgggtttgcc ggtcttcagg gcgcggcccc aggagtcggc acaggaggat
     2161 tcgccgacga cgctccagtc aaagcgctca tagcccgacc actgcaggcc gttgatgaag
     2221 tagatcatgg cgcctggcgt ggcgtagaac agcgcgatgt cgggttcgtc ggcgcccgcg
     2281 ccaaagtccg gccagcgctg cagcggcgcc accaccagcg cctggtagcg gcccacgggc
     2341 accacgtcca tcgcggcctg gtggcgcgcg gagtcctcca cggtggcaaa ccagacgccg
     2401 gtcatggcct ggcccgaata ccagtcggca tcctgtccgc ccaggccgac gacggcgcgg
     2461 cattgctcac ccaccaggtc agcggcctgc acgcccatgg tccagcccag gcgcgcggcc
     2521 tggccgacga tctggtccat ggtgtggatg gcctgcgggc gccgcagctt ggggatctgc
     2581 cgcagcgtct cggcggactc cagcagtttc atgccgatgg gcggagtgcg cagcttgagc
     2641 atgcgctcca gatcggcggc cagcgtgacg agttcatgtg cgttgctcat ggggtctcct
     2701 ggtgttgtga tggggcaagg aacagtcagt catccggccg ggccgcgccg cgccccacgc
     2761 gcagcgccag ccgcgagggc agggtggcca gcaccacgct ggccagggcc aggccaaagg
     2821 ccagcagctg cagcggcgtg aagcgctcgc ccagcaccag cacgccgacc agcgcggcgc
     2881 tgaccggcag cagcacggtg aacacgccgc cctgcgccgc gggcacggcg cgcaggccgg
     2941 tcatccacag ccacaccgtc cacatgcagg cggccagggc gtagaacagc agcagcagcc
     3001 agatgctcca gggcacggcc gcaaagtcaa aaccccacgc aaggtacagg ccgaacggca
     3061 tggacagcaa gaacccccac aggttgatca gcgacgtgat gcgcttgggg cccatggcgc
     3121 ccgtgagctt cttgccaatc accgaatagg ccccctcgca caccgaggcg cacagcagca
     3181 gggcgtagcc cagccactgc agatatatgg cgttatctgg ctccagcgcc gatccagatt
     3241 gcgcagggtg ctctggtttg gatagtgaaa acagcgcaat gccaatgacc gcacaggcca
     3301 cggccaccca ggtgcgccgc gccacgcgct cgcgcaggaa cagccagctc atcaccgcca
     3361 cgcaggcggg gatgcaggcc atgatcaccc cggccgaggt ggcgctggtc aggctgaccc
     3421 cgtagatcat gcacacggtg aacaaaaaat tgcccagcag cgattgcaga aacagcaggc
     3481 cgcgtgtctg cggcgtcagg ggcggctcgt cggcaggttt tttcagccag gtcagcatcg
     3541 ccagggcgcc aatgccaaag cgcatccagg ccagcagcag taccgggaac gcggccgcca
     3601 ggggtttgga caatgccaca taactgccca ccagcgacat gctgagagcg agacacagat
     3661 aggcgatggg gcggctgggg gcgttcactt cgggtcactg atgcacaaag acgcgcatca
     3721 tgcacaagcc agggcgtgcc gcgatggagt ggggctgcat ggattgcgac ggcttgtcag
     3781 ccttattttg caatgcggaa gtttgatttt gcattgtgaa gtttcgcgct tgctgcagcg
     3841 cagaattttt tctcaatatg agaaataaaa ttttacattg agatatttgt gatctatatg
     3901 gttgatttat aaagaaaaaa tttatctctt atataagaca taagagcaaa ttttggtctt
     3961 atagaagaca tagaattgcg tccaagggcc gcgcatagca gcccgccgat tcctcaagct
     4021 ttttttggag agcaaccatg tcgaactcgc tgaactcgca actgagccgt gaacaacaaa
     4081 tcgccgccct ggagaaggac tgggccacca acccgcgctg gaagggtgtc aagcgcggct
     4141 acagcgccgc tgacgtggtg cgcctgcgtg gcagcctgca gcccgagtac acgctggcca
     4201 agcgcggcgc cgagaagctg tgggagaagg tcaacggcgg cgccaagaag ggctatgtga
     4261 acgccttcgg cgccatcacc gccggccagg ccatgcagca ggccaaggca ggtctggagg
     4321 ccgtctacct gtcgggctgg caggtcgcag ccgacggcaa cacgtcggaa accatgtacc
     4381 ccgaccagtc cctgtacgcc tatgactcgg tgccgaccat ggtgcgccgc atcaacaaca
     4441 ccttcaagcg cgccgacgag atccagtggg gccgcggcat caaccccggc gacaagga
//
LOCUS       DAOMTQ010000199         4423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5203_length_4423_cov_28.393086, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000199 DAOMTQ010000000
VERSION     DAOMTQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5203_length_4423_cov_28.393086"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK518_14255"
     CDS             <1..508
                     /locus_tag="PK518_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HRM50120.1"
                     /translation="LQGEVAGDAAAARQARLLGAELELAAGDAPAALSYLEGSLALAD
                     ASQPDAPRRRPELLLRTQALLRTGAAAGMTAPLQTWLATHPGDATAWQALAQVWQQQG
                     QPLRAVRAEAEAQVARYDYAAAVDRFKAGQDLARRGGAANDHIEASIIDTRLRAVQSL
                     LKEQTAER"
     gene            complement(476..832)
                     /locus_tag="PK518_14260"
     CDS             complement(476..832)
                     /locus_tag="PK518_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRM50121.1"
                     /translation="MRLLLKWLLSAAALLCVAYVYSGVEVHSFGAALGAAFVIGLFNV
                     VLRPILVILTLPVTIVTIGLFLFVINALMFWAAASVLGDGFQVHGFTAALIGSLIYSL
                     LGLVIESALGGLFLQK"
     gene            complement(832..1596)
                     /locus_tag="PK518_14265"
     CDS             complement(832..1596)
                     /locus_tag="PK518_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRM50122.1"
                     /translation="MDLDFLTHTPFWIALGQIIIIDILLGGDNAVVIALACRKLPAAQ
                     RSKGIIWGTAGAIVLRVVLIIFAMTLLNLPFLKVVGALLLVWIGVKLLAPDEDAHGNV
                     AESDKLLAAIKTIIVADLVMSVDNVIAIAGAAQSSGEHSTLLVVLGLLISIPIIVWGS
                     QLVIKLMERFPMIITAGGMLLGWIAGGMLVTDPAFVNPDKWQWMPKLGTVDSNGMAEI
                     SRTLYWTAHVAGALLVLAIGKFITSRRGATDSAQHG"
     gene            2021..2431
                     /locus_tag="PK518_14270"
     CDS             2021..2431
                     /locus_tag="PK518_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HRM50123.1"
                     /translation="MTTIHITDIEAAINHWRACAWAPDGVALAPELSALAEVYGQMVF
                     ARAEEIAPGQLPAAAMAAWLAWYETQPDTPCIAICSTSQGDDCCKGCGRSFAEVQHWL
                     ELSPADKRAVWRRITREGRALRFTRYAERAAAAG"
     gene            2471..2776
                     /locus_tag="PK518_14275"
     CDS             2471..2776
                     /locus_tag="PK518_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRM50124.1"
                     /translation="MLRLTQAPDIATAMLWSDLLCEAGMPASVQRQHLGAAAGHLPPG
                     ECLPEIWLAHAEHADPARALLRDYERLPQRQWVCRACGESIEGGFEQCWNCGALMPR"
     gene            complement(2784..3344)
                     /locus_tag="PK518_14280"
     CDS             complement(2784..3344)
                     /locus_tag="PK518_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HRM50125.1"
                     /translation="MALKSTIFKANLSIADIDHGYYADHQLTLARHPSETDERMMVRL
                     LALALQAHELQDTCRGDGQLAFGAGLSDPDDPDVSLTDFTGRKRLWIEVGQPEDKPLS
                     KACSRADAVVVYAFAHAADVWWKGIQPKLTRLPKLQVWRVPSDAAQQLAALAERSMQL
                     QATVQEGALTLSSERGSVHVEPLRWK"
     gene            complement(3416..>4423)
                     /gene="purB"
                     /locus_tag="PK518_14285"
     CDS             complement(3416..>4423)
                     /gene="purB"
                     /locus_tag="PK518_14285"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRM50126.1"
                     /translation="INNTSHALQIRAGRDQVIVPGLDAIIAKLREMAHQHAQVPMLSR
                     THGQTASPTTVGKELANVVMRLQAATARITTVKILAKMNGAVGNYNAHLSAWPDFDWE
                     AFSQKVVETHEPQGLGLTFQPYSIQIEPHDYMAELFDAIARANTILIDLSRDIWGYVS
                     LAYFKQKLKEGEIGSSTMPHKVNPIDFENAEGNLGLANALLRHLAEKLPISRWQRDLT
                     DSTVLRNIGVAMGYAALAYSSLMTGLNKLELNQEALNADLDSSWEVLAEPIQTVMRRY
                     GVPGAYEKLKEVTRGKSVTAEALHALIRSLDIPQADKERLLALTPASYIGMATELARR
                     V"
BASE COUNT          722 a         1437 c         1525 g          739 t
ORIGIN      
        1 cttgcagggg gaggtggcgg gcgacgccgc cgccgcgcgc caggcgcgcc tgctgggggc
       61 ggagctggaa ctggcggccg gtgatgcgcc ggcggcgctg tcctatctgg agggcagcct
      121 ggcgctggcc gatgccagcc agccggatgc gcccaggcgc cggcccgaac tgctgctgcg
      181 cacccaggcg ctgttgcgca ccggcgccgc cgcaggcatg acggcgccac tgcagacctg
      241 gctggccacc cacccgggtg atgccacggc ctggcaggcc ctggcccagg tgtggcagca
      301 gcaggggcag ccgctgcgcg cggtgcgcgc cgaagccgag gcccaggtgg cgcgctacga
      361 ctacgccgcg gccgtcgatc gcttcaaggc cgggcaggac ctggccaggc ggggcggcgc
      421 ggccaatgac cacatcgagg cctcgatcat tgacacgcgg ctgcgcgccg tgcagtcact
      481 tctgaaggaa cagaccgccg agcgctgact cgatgaccag ccccagcagc gagtagatca
      541 gcgagccgat cagcgccgcc gtgaagccat gcacctgaaa accgtcgccc agcaccgagg
      601 ctgcggccca gaacatcagg gcgttgatga cgaacaggaa cagcccgatg gtgacgatgg
      661 tgaccggcag cgtgaggatc accaggatgg gtcgcaacac cacgttgaac agcccgatca
      721 cgaaggccgc ccccagggcg gcgccaaagc tgtgcacctc gacgccgctg tagacatagg
      781 ccacgcacag cagcgccgca gcgctgagca gccatttgag cagcagccgc atcaaccgtg
      841 ctgcgccgaa tcggtggcac cgcggcgcga ggtgatgaac ttgccgatgg ccagcaccag
      901 cagcgcgccg gcgacatggg ccgtccagta cagggtgcgc gagatctcgg ccatgccgtt
      961 gctgtccacc gtgcccagct tgggcatcca ttgccatttg tcggggttca cgaaggccgg
     1021 gtcggtgacc agcatgccgc cggcgatcca gcccagcagc atgccgccgg cggtgatgat
     1081 catcgggaag cgctccatca gcttgatcac cagctggctg ccccagacga tgatgggaat
     1141 cgagatcaac agacccagca ccaccagcag cgtggaatgc tcgcccgagc tttgcgcggc
     1201 gccggcgatg gcgatcacgt tgtccacgct catcaccagg tcggcgacga tgatggtctt
     1261 gatggcggcc agcagcttgt cgctttcggc cacattgccg tgggcgtcct catcgggcgc
     1321 cagcagcttc acgccaatcc acaccagcag cagcgcaccc accaccttca ggaagggcag
     1381 gttcagcagc gtcatcgcaa agatgatgag caccacgcgc aggacgatgg cgccggccgt
     1441 gccccagatg atgcccttgg agcgctgggc cgccggcagc ttgcggcagg ccagggcgat
     1501 caccaccgcg ttgtcgccgc ccagcaggat gtcgatgatg atgatctgcc ccagcgcgat
     1561 ccagaacggc gtgtgggtga gaaagtccaa atccatatgt ctttacctcg atgtcgaatg
     1621 ccggttcggg ggaactcggc ggcgttcaaa taccgggcag catggaaagc gtggggcaat
     1681 cgcctgcgtg caagccttgc ctgcttgcag aaaaacggat gcgctttgag ccaaccatgg
     1741 gtgcctgtgg ctcaaaggtc ttgctcggcg atggcgctgg ccttgcctga caggccggaa
     1801 gcacatggct tcgtattgac gacctgacat ccgtttgcag gcggccctga cgcaaggcct
     1861 tgtgcaaaac ggggaactac tcccccttga ggggcgccat ttgaccacag tttggcgatt
     1921 cgtgggcaag aacgttgggg gccgggcggc atgtcgcacc agggtcgggg cactatcatg
     1981 gccgcctttt cgtgaccgca tcgttgcccg ctcaccgcgc atgaccacca tccacatcac
     2041 cgatatcgag gccgccatca accattggcg tgcgtgcgcc tgggcgcccg atggcgtggc
     2101 gctggcgccc gagctctcgg ccctggccga ggtctacggg cagatggtgt tcgcgcgtgc
     2161 cgaggagatt gcgccggggc agttgcccgc agccgccatg gccgcgtggc tggcctggta
     2221 cgaaacccag cccgacacac cctgcatcgc gatctgctcc accagccagg gcgatgattg
     2281 ctgcaagggc tgcggccgca gctttgccga ggtgcaacat tggctggagc tgagccccgc
     2341 ggacaagcgc gccgtgtggc gccgtatcac gcgcgagggc cgcgccctgc gctttacgcg
     2401 ctatgccgag cgcgcggcgg cagcaggctg aggctttacg gcgcggcgtc gcgcggctat
     2461 cgtggggcgc atgctgcgtc tgacccaggc ccccgacatt gccacggcca tgctgtggag
     2521 cgatctgctg tgcgaggccg gcatgcccgc cagcgtgcag cgccagcacc tgggcgcggc
     2581 ggccggccat ttgccacccg gtgaatgcct gcccgagatc tggctggcgc atgcggagca
     2641 tgccgatccg gcccgcgccc tgctgcgtga ctacgagcgc ctgccccagc gccagtgggt
     2701 ctgccgcgcc tgtggcgagt cgattgaggg cggtttcgag caatgctgga actgcggcgc
     2761 cctgatgccg cgctgaagcc gggttatttc cagcgcagcg gctccacatg cacgctgccg
     2821 cgctcgctgc tgagcgtgag cgcgccctcc tgcaccgtgg cctgcagctg catgctgcgc
     2881 tcggccaggg cggcgagctg ctgcgcggca tcagagggca cgcgccagac ctgcagcttg
     2941 ggcaggcgcg tgagcttcgg ttggatgccc ttccaccaca catccgccgc atgggcaaag
     3001 gcgtagacca ccaccgcgtc ggcgcggctg caggccttgg acagaggctt gtcctcgggc
     3061 tggccgacct ctatccacaa gcgcttgcgg cccgtgaagt cggtcagcga cacgtccgga
     3121 tcgtcggggt cggacaggcc ggcgccaaag gccagctggc cgtcaccacg gcaggtgtcc
     3181 tgcagctcat gggcctgcag cgccagggcc agcaggcgca ccatcatgcg ctcgtcggtc
     3241 tcgctggggt ggcgcgccag ggtcagctgg tggtcggcgt agtagccatg gtcgatgtcg
     3301 gcgatggaca gattcgcctt gaagatggtg gacttgaggg ccatgaggtt tctcaaaaat
     3361 aagagctgcc cgcgcttgac tggcaagggc gggcagctaa aaagactgaa aaaagtcaga
     3421 cgcgtcttgc cagctcggtg gccatgccga tgtagctcgc cggcgtcagc gccagcaggc
     3481 gctccttgtc cgcctggggg atgtccaggc tgcgaatcag cgcgtgcagg gcctcggcgg
     3541 tcacgctctt gccgcgcgtg acctccttga gcttttcata ggcgcccggc acgccatagc
     3601 ggcgcatgac ggtctggatg ggctcggcca gcacttccca gctgctgtcc aggtcggcgt
     3661 tcagcgcttc ctggttgagt tccagcttgt tcaggccggt catcagcgag ctgtaggcca
     3721 gggctgcata gcccatggcc acgccgatgt tgcgcagcac ggtgctgtcg gtgaggtcgc
     3781 gctgccagcg gctgatgggc agcttttccg ccagatggcg caagagcgcg ttggccaggc
     3841 ccaggttgcc ctcggcgttc tcaaagtcga tcgggttgac cttgtgcggc atggtgctgg
     3901 acccgatctc gccttccttc agcttctgct tgaagtaggc cagcgagaca taaccccaga
     3961 tgtcgcgcga gaggtcgatc agtatggtgt tggcccgcgc gatggcgtcg aacagttcgg
     4021 ccatgtagtc gtgcggctcg atctggatgc tgtagggctg gaaggtcagg cccaggcctt
     4081 gcggttcgtg cgtttccacg accttctggc tgaaggcctc ccagtcgaag tcgggccagg
     4141 ccgacagatg ggcgttgtag ttgcccacgg cgccgttcat cttggccagg atcttcaccg
     4201 tggtgatgcg cgccgtggcc gcctgcaggc gcatgaccac gttggccagc tccttgccca
     4261 cggtggtcgg gctggccgtc tggccatggg tgcggctgag catgggcacc tgggcatgct
     4321 ggtgcgccat ctcgcgcagc ttggcgatga tggcgtccag gccaggaaca atgacctggt
     4381 cgcgcccggc gcggatctgc agcgcgtggc tggtgttgtt gat
//
LOCUS       DAOMTQ010000200         4423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5204_length_4423_cov_25.951465, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000200 DAOMTQ010000000
VERSION     DAOMTQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5204_length_4423_cov_25.951465"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PK518_14290"
     CDS             complement(<1..804)
                     /locus_tag="PK518_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM50127.1"
                     /translation="MSTSNQNLFAALRAAFPADLEQIAVETMSASGEPLLYSWRDLDR
                     ASARIANLLAALELPEGSRIAVQVEKSVEAMLLYLATLRAGHVFLPLNTAYQSAEIEY
                     FVGNAEPAVVVCTPGNFGWVSKIAFTAGTQHVFTLGDDRTGSLLERAAHFGDDHVPVA
                     RGADDLAAILYTSGTTGRSKGAMLTHGNLLSNAVMLKDYWGWVPGDVLIHALPIFHVH
                     GLFVAIHAALLNGSKMIWLAKFDPRAAIAAMARATVFMGVPTLYVRMLAE"
     gene            complement(823..1638)
                     /locus_tag="PK518_14295"
     CDS             complement(823..1638)
                     /locus_tag="PK518_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRM50128.1"
                     /translation="MSGEVVAAMAAAEAAGRGVLRVTLRHDGRLNAMSRAMWRHLRSV
                     FESVQRSIDVRCVLIEGAGQAFCAGGDISEYPDFRFDVAQLRDFHENDVWGGLSAMLA
                     CDVPIVARIAGACMGAGVEIASCCDIRIAETGARFGAPIAKLGFPMAPREAALVAGAV
                     GDVTARQMLLEAATFDAAEMARRGFLSRVVEADQLEAQAVGAALRIAALAPGAARMNK
                     QTLRAFQAQKMPTTPDHQAQIAIKTIVDPYAYADSAEHREGITAFLEKRSPVF"
     gene            complement(1668..2690)
                     /locus_tag="PK518_14300"
     CDS             complement(1668..2690)
                     /locus_tag="PK518_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM50129.1"
                     /translation="MQHQDHTTRFTQLSSRRDLLRTAGAAGLALATGAAWAQGASGWP
                     SKPVNLIVPFPAGGGTDAFARPLAAQFSKSTGKTLVIDNRGGAGGTVGASAAAKAPAD
                     GYNLFMGGVHHVIAPSMYPRLDYDIEKDFIPLALLAKVPQVLVVNPRNVTVSDFRQFM
                     ELVKANPAKLNYASAGAGTSHHLAGELFKLQTGTFITHIPYRGAGPALQDLIAGNVDM
                     LFDGLGSSAAHIKGGRIRALMVSGASRNPAFPDVPCAAELGLPDYTVSTWYGLWAPKG
                     TPADVQARIVEEMRKLGNAEEIKTIWAGNGAEFGQLSQADFQKMVSGEVKRWAQVVKA
                     SGARLE"
     gene            complement(2739..4211)
                     /locus_tag="PK518_14305"
     CDS             complement(2739..4211)
                     /locus_tag="PK518_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HRM50130.1"
                     /translation="MNNATSAWISRSVDRLRPAKGKAAAAKDKPGDGPGEPQSGRSTH
                     ERLAATLRRGGEALSPRALRQLLAQLQDVLAPRVSEIEGGRRAQAVATWYGQAEPAQR
                     RDLWLLMGEQFVPDAARMKNAQQRYQAALGTADEPQAEAQLRRALNSPRTRLLQRFAA
                     FPEGMRFLVDLRAELLPHLKADQCLLPLEAELEQLFSTWFDVAFLELRRLSWDSPASL
                     IEKLIKYEAVHDIRSWDDLKNRLDSDRRCYGFFHPRLPNEPLIFVEVALTDEISDSIT
                     PLLDESAAPSDIQRASTAIFYSISNTQAGLRGVSFGDSLIKHVVETLQQEFPRLRHFA
                     TLSPIPGFRAWLQKNCATQLELLDAKRLAELGRAVGFEPKGAKDLPAQVLAAADKALE
                     LAPRSALRQLLQQWAARYLARELQDGKPLDPVARFHLGNGARVERLNWAADPSAKGLK
                     QSFGLMVNYLYDLKRIDKHRDLLAQAKVPVSGDIEDLCRP"
     gene            complement(4208..>4423)
                     /locus_tag="PK518_14310"
     CDS             complement(4208..>4423)
                     /locus_tag="PK518_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRM50131.1"
                     /translation="GDLRKILRLARHQTLHVPGRLQQSLAEHLQVFAALKSGDAGAAE
                     QAMRQHLLAQREALRELARNHPSRIAP"
BASE COUNT          702 a         1496 c         1516 g          709 t
ORIGIN      
        1 ctcggccagc atgcgcacgt acagcgtggg cacgcccatg aacacggtgg cgcgcgccat
       61 ggcggcgatg gccgctcggg gatcgaactt ggccagccag atcatcttgc tgccattcag
      121 cagcgcggcg tggatggcca cgaacaggcc atgcacatgg aagatgggca gggcgtggat
      181 gagcacgtcc ccaggcaccc agccccagta gtccttgagc atcaccgcgt tggacagcag
      241 attgccgtgc gtcagcatcg cccccttgct gcgcccggtg gtgccgctgg tgtagaggat
      301 ggcggccagg tcgtcagcgc ccctggcgac tgggacatgg tcgtcgccaa agtgcgcggc
      361 ccgctccagc aggctgcccg tgcggtcgtc gcccagcgtg aacacatgct gcgtaccggc
      421 ggtaaacgcg atttttgaca cccagccgaa gttgcccggc gtgcagacca ccacggccgg
      481 ctcggcattg ccgacgaagt attcgatctc ggcgctctgg taggcggtgt tgagcggcaa
      541 gaacacatgg cccgcgcgca gcgtggccag gtacagcagc atggcctcga ccgatttttc
      601 cacctgcacg gcgatgcggc tgccctcggg cagctcgagc gcggccagca ggttggcgat
      661 gcgggcgctg gcgcgatcga ggtcgcgcca gctgtagagc agcggctcgc cgctggcgga
      721 catggtctcc accgcgatct gctccaggtc ggcgggaaag gcggcgcgca gagcggcaaa
      781 gaggttctgg ttggaggtgc tcatggtgtg tgcaagccaa aatcaaaata caggggagcg
      841 cttctccagg aaggcggtga tgccctcgcg gtgctcggcg ctgtcggcat aggcgtacgg
      901 atcaactatt gttttaatag ctatttgcgc ttggtggtcg ggcgttgtag gcattttttg
      961 tgcttgaaac gcgcgcaggg tctgtttgtt catgcgcgcc gcacccggcg ccagcgcggc
     1021 aatgcgcagc gcggcgccaa cggcctgggc ctccagctgg tcggcctcga cgacgcggct
     1081 cagaaagccc cgtcgggcca tctcggcggc atcgaaagtg gcggcctcca gcaacatctg
     1141 gcgcgcggtc acgtcgccca ccgcgccggc caccagggcc gcctcgcgcg gcgccatcgg
     1201 aaagccgagc ttggcgatgg gcgcgccaaa gcgcgcgccg gtttcggcga tgcggatgtc
     1261 gcagcagctg gcgatctcca cgcccgcgcc catgcaggcg ccagcaatgc gcgcgacgat
     1321 gggcacgtcg caggccagca tggccgacag gccgccccag acgtcgtttt cgtgaaagtc
     1381 acgcagctgc gcgacatcga agcgaaagtc cgggtactcg gagatgtcac cgcccgcgca
     1441 gaacgcctgc cctgcgccct ctatgagcac gcagcgcacg tcaatactgc gctgaacgct
     1501 ctcgaaaaca gagcgcaagt ggcgccacat ggcgcggctc atggcgttca gccgcccatc
     1561 gtggcgcagc gtcacgcgca gcacgccgcg gccggcggcc tccgctgcgg ccatggccgc
     1621 gacaacttca cccgacatgc aatcgctctt tctctcgttg aatgaactca ctccagcctg
     1681 gcgcccgatg ccttgaccac ctgcgcccag cgcttgacct cgccactgac catcttctgg
     1741 aagtccgcct gcgacagctg gccaaactcg gcgccgttgc cggcccagat ggtcttgatc
     1801 tcctcggcgt tgcccagctt gcgcatctct tcgacgatgc gcgcctgcac gtcggccggc
     1861 gtgcccttgg gcgcccacag gccgtaccag gtggagacgg tgtagtccgg caggcccagc
     1921 tcggcggcgc agggcacgtc ggggaaggcc gggttgcggc tggcgcccga gaccatcagc
     1981 gcccggatgc gcccgccctt gatatgcgct gccgacgagc ccaggccgtc gaacagcatg
     2041 tccacgttgc cggctatcaa gtcctgcagc gccgggcccg cgccgcggta cggaatgtgg
     2101 gtgatgaagg tgcccgtctg cagcttgaac agctcacccg ccagatggtg cgaggtgccc
     2161 gcgccagcgg atgcgtagtt gagcttggcc ggattggcct tgaccagctc catgaactgc
     2221 ctgaaatcgc tcaccgtgac gttgcgcggg ttcaccacca gcacctgcgg caccttggcc
     2281 agcagggcca gggggatgaa gtccttctcg atgtcgtagt ccagccgcgg gtacatggag
     2341 ggcgcgatca catggtgcac gccgcccatg aacaggttgt agccatcggc cggcgccttg
     2401 gccgccgcgc tggcgcccac cgtgccgccc gcgccgccgc ggttgtcgat gaccagggtc
     2461 ttgcccgtgg acttggaaaa ctgcgccgcc agcgggcgcg caaacgcatc cgtgccgcca
     2521 cctgccggaa agggcacgat caggttcacc ggcttggacg gccagccgct ggcgccctgc
     2581 gcccaggcag cgcccgtggc cagggccagg cccgccgccc cggccgtgcg caacaggtcg
     2641 cgccggctcg acagctgcgt gaagcgcgtg gtgtgatcct ggtgttgcat gaatttgtct
     2701 ccttggtgtt atgaaaagaa tgccctgccc gcactgcgtc aagggcggca cagatcctcg
     2761 atgtcgcccg acaccggcac cttggcctga gccagcaggt cgcggtgctt gtcgatgcgc
     2821 ttcaagtcgt acaggtagtt gaccatcagt ccaaaggact gcttcaggcc cttggccgag
     2881 gggtccgcgg cccagttcag ccgctccacg cgcgcgccgt tgcccaggtg aaaacgcgcc
     2941 acgggatcca gcggcttgcc atcctgcagc tcgcgcgcca ggtagcgcgc ggcccattgc
     3001 tggagcagct ggcgcagcgc cgaccggggc gccagctcca gcgccttgtc ggctgcggcc
     3061 agcacctggg ccggcaggtc cttggcgccc ttgggctcaa agccgacggc gcggcccagc
     3121 tcggccaggc gcttggcgtc cagcagctcc agctgcgtcg cgcagttctt ctgcagccag
     3181 gcgcgaaagc ccgggatggg cgagagcgtg gcgaaatggc gcaggcgcgg aaactcctgc
     3241 tgcagcgtct ccaccacatg cttgatcagc gagtcgccaa agctcacgcc gcgcagcccg
     3301 gcctgggtgt tgctgatgga gtaaaaaatc gccgtgctgg cgcgctgtat gtcagacggc
     3361 gcggccgatt cgtccagcag cggtgtgatg ctgtcggaga tttcatccgt cagcgccacc
     3421 tcgacgaaga tcagcggctc attgggcagg cgcgggtgaa agaagccata gcagcgccgg
     3481 tcgctgtcca ggcgattctt caggtcgtcc cagctgcgga tgtcgtgcac cgcctcgtac
     3541 ttgatgagct tttcgatcag cgacgcgggc gagtcccagg acaggcgtct gagctccaga
     3601 aaggcgacgt caaaccaggt ggagaacaac tgctccagct cggcctccag cggcaacagg
     3661 cattggtcgg ccttcagatg gggcagcagc tcggcgcgca agtccaccag aaaacgcatg
     3721 ccctcgggaa aggcggcaaa gcgctgcagc agccgcgtgc gcggcgaatt cagcgcgcgg
     3781 cgcagctggg cctcggcctg gggctcgtcg gccgtgccca gggcggcctg gtaacgctgc
     3841 tgggcgttct tcatgcgcgc ggcgtcgggc acgaactgtt cgcccatcag cagccacaga
     3901 tcgcgccgct gggccggctc ggcctggcca taccaggtgg ccacggcctg ggcgcggcgc
     3961 ccgccctcga tctcgctcac gcgcggcgcc agcacatcct gcagctgggc cagcagctga
     4021 cgcagcgcgc gcggcgacaa ggcctcgccg ccacggcgca gcgtggccgc caggcgctcg
     4081 tgcgtactgc gcccggactg gggctcgccc gggccgtccc cgggcttgtc cttggccgca
     4141 gctgccttgc ctttggcggg ccgcaggcga tcgacgctgc gcgaaatcca ggcggaggtg
     4201 gcgttgttca tggtgctatg cgggaggggt ggttgcgggc cagctcgcgc agcgcctcgc
     4261 gctgggccag aagatgctgg cgcatggcct gctcggcggc gccggcgtcg ccgctcttga
     4321 gcgcggcaaa cacctgcaga tgctcggcca ggctttgctg caggcggccc ggcacatgca
     4381 gcgtctggtg gcgcgccagg cgcaggatct tgcgcaggtc gcc
//
LOCUS       DAOMTQ010000201         4419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5211_length_4419_cov_29.051329, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000201 DAOMTQ010000000
VERSION     DAOMTQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_4419_cov_29.051329"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..948)
                     /locus_tag="PK518_14315"
     CDS             complement(22..948)
                     /locus_tag="PK518_14315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM50132.1"
                     /translation="MPLAHIALPPEIIVMERSWLSANNLLFLGRDATALVDSGYCSNA
                     AQTVALVQSSLGGRPLDLLLNTHLHSDHCGGNAALQARWPAVQTWIPPGQWDQVQDWD
                     PVALSYAPTGQDCPRFRAEARLMPGSEVQLGDRAWQVHAAPGHDNHAVLLFEPRARLL
                     ISGDALWENGFGVVFPELDGEDAFAEVGATLDLIARLQPLTVIPGHGGVFADVPEAIA
                     RARRRLAGFEADPVRHARYAAKVLLKYKLLEWQQAPLTQVHAWLAATPYYARLHQRYF
                     ADRMAGQWTEELVQELIATGAARREGETLHNA"
     gene            complement(974..2659)
                     /locus_tag="PK518_14320"
     CDS             complement(974..2659)
                     /locus_tag="PK518_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM50133.1"
                     /translation="MDRIWLKNYPPGVPHDVDADQYQSVAQMLDESFARHADRPFSVC
                     MDRWLSYGALDRLSGQLGAWLQGQGLAPGARVAIMLPNVPQFAVTMAAVLRAGYTCVN
                     VNPLYTARELQHQLQDSGASAIVILENFAHTLAEVVELSAVQHVVIASMGDLLGPWYG
                     RWISFAVRHLAKMVPAYELPLAGGRQVLSFKRALALGARRRLRPAQVTPDTPAFLQYT
                     GGTTGLSKGAVLTQRNIIAATLQAESWFSPALARVGDAGRINSIAALPLYHIFALTLC
                     LLALRQGSRLTLIPNPRDIPKFVEVLKKRPFHLLPAVNTLFNALLQNDRFRQLDFSQL
                     CVSQAGGMAASESTAQQWHRLTGSTMIEGWGMSETCAIGTNNPVNASSFSGSIGLPLP
                     GIDIAIKDDAGASLATGQAGEICIRGPNVMPGYWNQPEENARTFTADGFMRTGDIGIM
                     DEGGYTRIVDRKKDMILVSGFNVYPNELEQVVALCPGVLECAAVGVPDEAQGEAIKIF
                     VVRSDPALTEDEVARHCHDQLTGYKRPRYIEFRDELPKTNVGKILRRELRAHA"
     gene            2753..3676
                     /locus_tag="PK518_14325"
     CDS             2753..3676
                     /locus_tag="PK518_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM50134.1"
                     /translation="MTNARWQRWLVAWHGVLVLVWLQGFWQRSVPLALAGLVAVPLLS
                     RLTMLPQFLLMAWVCRHDAAPRLGAARLLRAWWAESRWAALVFGWWQPFRMHAVPDWL
                     PPRAADRPAPRGVVLVHGFLCNRAFWTPWFAPLRARGHAFVAVTLEPAFGSIDDYAPA
                     IEAAVRRVTEATGQAPLIVGHSMGGLAIRAWLRATPDGDARVHRVVTIGSPHHGTWPA
                     AHARSIPGQQMRLHGPWVQQLAAHEPPARRALFRCWYSNGDNAVYPPAAATLAGADNR
                     FVASLAHVELAFDPRVLADCLELIKLGSDSN"
     gene            3851..>4419
                     /locus_tag="PK518_14330"
     CDS             3851..>4419
                     /locus_tag="PK518_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRM50135.1"
                     /translation="MPPFSALKKPKSLRMAAALLPLSAMAQQAPAPAPVHTLPAVTVQ
                     EQAIDPNPNAEVGAPYKARTSADTRHTRPLAETPQTIQVVTGEAIRDSGQTDLKSILA
                     TQPGITLGTGENGNAFGDRYIIRGQEARSDVFVDGLRDPGMTTRESFAIEQVEISKGP
                     NSSFAGRGSAGGAVNAVTKQATLDYDFTR"
BASE COUNT          683 a         1513 c         1494 g          729 t
ORIGIN      
        1 atctgacccc aattaatgcg atcaagcgtt gtgtagcgtc tcgccctcgc gtcgcgcggc
       61 gccggtggcg atgagttcct gcaccagctc ctctgtccac tggccggcca tgcgatcggc
      121 aaagtagcgc tggtgcagcc gcgcgtaata gggcgtggcg gccagccagg cgtggacctg
      181 ggtcagcggg gcctgctgcc attcgagcag cttgtatttc agcagtacct tggcggcgta
      241 gcgggcatgt ctcacggggt cggcctcgaa gcccgccagg cgccggcgcg cgcgcgcgat
      301 ggcctcgggc acgtcggcaa acacgccgcc atggccgggg atgacggtca gcggctgcag
      361 gcgcgctatc agatccagcg tggcacccac ctcggcaaag gcgtcttcac cgtcgagctc
      421 ggggaacacc acgccaaagc cgttttccca tagcgcgtcg cccgagatca gcaggcgtgc
      481 gcgcggctcg aacagcagca cggcgtggtt gtcgtggccc ggcgcggcgt gcacctgcca
      541 ggcgcgatct ccgagctgca cctcgctgcc gggcatcagg cgcgcctcgg cgcgaaagcg
      601 cgggcagtcc tggcccgtgg gtgcgtagct cagcgccacc gggtcccagt cctgcacctg
      661 atcccactgg cccggtggga tccaggtctg cacggccggc cagcgcgcct gcagcgcggc
      721 gttgccaccg caatggtcgc tgtgcagatg ggtgttgagc agcagatcca gcggccgccc
      781 gcccaggctg gactgcacca gcgccacggt ctgcgcggcg ttgctgcaat agccgctatc
      841 gaccagggcc gtggcgtcgc gccccaggaa cagcaggttg ttggccgaca gccagctgcg
      901 ctccataaca atgatttctg gtggtagggc aatatgggca agcggcatca gctattaaat
      961 agatagtaaa atttcaggcg tgggcgcgca gttcgcgccg cagtatcttg cccacattgg
     1021 tcttgggcag ctcgtcgcgg aactctatgt accgggggcg cttgtagccg gtcagctggt
     1081 cgtggcaatg gcgcgccacc tcgtcctcgg tcagggccgg gtcgctgcgc acgacgaaga
     1141 tcttgatggc ctcgccctgg gcctcgtcgg gcacgcccac ggccgcgcat tcgagcacgc
     1201 cggggcacag cgccaccacc tgctcgagct cgttgggata gacgttgaag ccgctgacca
     1261 ggatcatgtc cttcttgcgg tccacgatgc gcgtgtagcc gccctcgtcc atgatgccga
     1321 tgtcgcccgt gcgcatgaag ccgtcggccg tgaaggtgcg cgcgttttcc tcgggctgat
     1381 tccagtagcc gggcatgacg ttggggccgc ggatgcagat ctccccggcc tggccggtcg
     1441 ccaggctggc gccggcgtcg tccttgatgg cgatgtctat gcccggcagc ggcaggccga
     1501 tgctgccgct gaagctgctg gcgttgaccg ggttgttggt gcctatggcg caggtctcgc
     1561 tcatgcccca gccctcgatc atggtgctgc cggtgagcct gtgccattgc tgcgccgtgg
     1621 actcgctggc ggccatgccg ccggcctggg agacgcagag ctgcgaaaaa tcgagctgcc
     1681 ggaacctgtc gttctgcagc agtgcgttga acagcgtgtt caccgccggc agcaggtgga
     1741 aggggcgctt cttcagcacc tcgacaaact tcggtatgtc gcgcgggttg ggaatcagcg
     1801 tcaggcgcga gccctggcgc agcgccagca ggcacagcgt gagggcgaag atgtggtaca
     1861 gcggcagcgc ggcgatgctg ttgatgcgac cggcgtcacc cacccgggcc agcgccgggc
     1921 tgaaccagct ttctgcctgc agcgtggcgg cgatgatgtt gcgctgcgtc agcaccgcgc
     1981 ccttggacag cccggtggtg ccgcccgtgt actgcagaaa ggccggcgta tcgggcgtga
     2041 cctgcgccgg ccgcagcctg cggcgcgcgc ccagggccag ggcgcgcttg aaactcagca
     2101 cctggcgccc gccggccagc ggcagctcat aggcgggcac catcttcgcc aggtggcgca
     2161 cggcaaagct gatccagcgc ccgtaccagg ggccgagcag gtcgcccatc gatgcgatca
     2221 ccacatgctg cacggcgctg agctctacca cctcggccag cgtgtgtgcg aaattctcca
     2281 ggatcacgat ggcgctggcg cccgagtcct gcagctggtg ctgcagctcg cgcgcggtgt
     2341 acagcgggtt cacgttcacg caggtgtagc cggcgcgcag cacggccgcc atggtcacgg
     2401 cgaactgcgg cacattgggt agcatgatgg ccacgcgcgc gcccggcgcc aggccctggc
     2461 cctgcagcca ggcgcccagc tggccggaca ggcggtcgag cgcgccatag ctcagccagc
     2521 gatccatgca caccgaaaac ggcctgtcgg cgtggcgcgc aaaggactcg tcgagcatct
     2581 gcgccaccga ctggtattgg tcggcatcaa catcgtgggg cacgcccggg gggtaattct
     2641 tgagccagat tctgtccatg aggagccttg tgaagatcgc acgcattgtc aaagtgcgcg
     2701 ccgcgccgcc ctaccgggct tgtcctatgt ttggtcactc cggagacagc cggtgacgaa
     2761 cgcgcgctgg cagcgctggc tggtggcctg gcacggggtg ctggtgctgg tctggctgca
     2821 gggcttctgg cagcgctcgg tgccgctggc gctggccggg ctggtggccg tgccgctgct
     2881 gtcgcggttg accatgctgc cgcaattcct gctcatggcc tgggtctgcc ggcacgatgc
     2941 ggcgccacgc ctgggcgccg cacggctgct gcgcgcctgg tgggccgagt cgcgctgggc
     3001 cgccctggtg tttggctggt ggcagccgtt tcgcatgcat gccgtgcccg actggctgcc
     3061 gccgcgtgcg gccgataggc ctgcgccgcg cggcgtggtg ctggtgcatg gctttctgtg
     3121 caaccgggcc ttctggacgc cctggttcgc accgctgcgc gcgcgcggcc atgcctttgt
     3181 cgccgtgacg ctggagccgg ccttcggctc catcgacgat tacgcgccag ccatagaagc
     3241 cgccgtacgc cgcgtcaccg aggccaccgg tcaggccccg ctcatcgtcg gccacagcat
     3301 gggtggcctg gcgatacgcg cctggctgcg cgccacgccc gatggcgatg cgcgcgtgca
     3361 ccgcgtggtc acgattggtt cgccacacca cggcacctgg cctgccgccc atgcgcgcag
     3421 catccccggc cagcagatgc gcctgcacgg cccctgggtg cagcagcttg cggcgcacga
     3481 gccgcccgcg cgcagggcgc tgttccggtg ctggtacagc aacggcgaca acgccgtcta
     3541 tcccccggct gccgccacgc tggcaggcgc ggacaaccgc ttcgtcgcca gcctggcgca
     3601 tgtggagctg gcctttgacc cacgcgtgct ggccgattgc ctggaactga ttaaattggg
     3661 gtcagattcc aattaaccgg caatcaaagc aagcacccca ggcgtcagcc cacgttgcct
     3721 gacagcaaca tcacaccgcc gacgaaccag ccatcacccg gtgaatgcga ctttttcgcg
     3781 tcgattattg caaatgcgat tgatttgcaa taataatcca gcccgaaatt ccgctactgt
     3841 tgagacgcac atgccaccct tttccgccct gaagaaaccc aaaagcctgc gcatggccgc
     3901 cgcgctgctg ccgctgtcgg ccatggccca gcaggcaccg gcgcccgcgc cggtgcacac
     3961 cctgcccgcc gtgacggtgc aggagcaggc catagacccc aaccccaacg ccgaggtggg
     4021 tgcgccctac aaggccagga cctcggccga cacgcgccac acgcgcccgc tggccgagac
     4081 gccgcagacc atccaggtgg tgacgggcga ggccatccgc gactccggcc agaccgacct
     4141 gaagagcatt ctggccaccc agcccggcat caccctgggc acgggcgaga acggcaacgc
     4201 ctttggcgac cgctacatca tccgcggcca ggaggcgcgc tcggacgtgt tcgtggacgg
     4261 cctgcgcgac cccggcatga ccacgcgcga gagctttgcc atcgagcagg tcgaaatctc
     4321 caagggcccc aactccagct ttgccgggcg cggctcggcc ggtggcgcgg tgaacgccgt
     4381 gaccaagcag gccacgctgg actatgactt cacgcgcat
//
LOCUS       DAOMTQ010000202         4399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5254_length_4399_cov_28.655617, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000202 DAOMTQ010000000
VERSION     DAOMTQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5254_length_4399_cov_28.655617"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1029)
                     /gene="rsmI"
                     /locus_tag="PK518_14335"
     CDS             complement(106..1029)
                     /gene="rsmI"
                     /locus_tag="PK518_14335"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRM50136.1"
                     /translation="MSASFASALHAARDAAAAQHYPQGALYVVATPIGNLADISLRAL
                     HVLQLADTIACEDTRHTQALLRAYGIDKPGERLLALHQHNEVEAAQTVLQRLQAGQRV
                     AYVSDAGTPAVSDPGARLVAAVQAAGLRCMPLPGASSVTSALSVSGAIAHASENQGFV
                     FAGFLPSRSAERNSAIQRLAAEPRCVLLLEAPHRILELAQALAMLGERRVTLARELTK
                     QFEEVTTHAAQDLAAWLQGAPQRSKGEFVVLLHPAPVPQDDDAAAERVLRLLLAELPT
                     KTAVKLTADITNAPRNALYELALQIKRSEQD"
     gene            1029..1427
                     /locus_tag="PK518_14340"
     CDS             1029..1427
                     /locus_tag="PK518_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRM50137.1"
                     /translation="MGFLGKKIMGGAPRHTTRAIGQVAEDRALACLVAAGLVLVERNY
                     RTPGRGGGEIDLVMRERDGTLVFVEVRSRGAGDFGGAAASIGATKRRRIILAAQHYLL
                     RWSAPPPCRFDAVLIDDGRVQWLRAAFDAG"
     gene            1488..2090
                     /locus_tag="PK518_14345"
     CDS             1488..2090
                     /locus_tag="PK518_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM50138.1"
                     /translation="MLEQRIQQHFIDSADLKYQAAQALSQPITAAVQAIWACVTNGAK
                     VLACGSGPSAAQAQQLAAFCVTGFERERPELAALALVPDALWLAAATTGAGADIAALA
                     RQVRALGQAGDLLLAITIAGNEPAMLEAVQAAHERDMTVVALTGRDGGALATLLRETD
                     VQICVPHDRPARVREVHALVLHCLCDGVDAQLLGEQENPI"
     gene            2087..2743
                     /locus_tag="PK518_14350"
     CDS             2087..2743
                     /locus_tag="PK518_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRM50139.1"
                     /translation="MKMQSMRTLGALLAAASLVGSLAACVPLVVGGGAVVGTMMAVDR
                     RTAGTQVEDEGIELRAVNRIREAMGERAHVNVTSYNRQVLLTGEVPTAADSQKVEQIV
                     LAVDNVRSVVNDLGVMANTSLSQRSKDTFITGKVRASLVDAKDLTANAFKVVTERGVV
                     YLMGRVTQREAKRSAEIARGVDGVHKVVRVFEVISEEELARGVAAQPAPVSQDPAAAR
                     "
     gene            complement(2747..3673)
                     /locus_tag="PK518_14355"
     CDS             complement(2747..3673)
                     /locus_tag="PK518_14355"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM50140.1"
                     /translation="MSSINPRSYEPSPTRRVAFLGLGVMGYPMAGHLATAGHQVTVYN
                     RTATKSEAWCAEFAGVTGQKHAKTPREAAQDAEIVFCCVGNDEDLRSVVLGADGAFAG
                     MRPGATFVDHTTASAEVARELYAAAKKLGLQFIDAPVSGGQAGAQNGQLTVMCGGDQA
                     AFDAMRPTAMAFSRACTRIGDSGAGQLAKMVNQICIAGLVQGLSEAIAFGQQAGLDMP
                     LVLDVIGKGAAQSWQLDNRGKTMVQGRFDFGFAVDWMRKDLGLVLDEARRNGARLPVT
                     ALVDQFYADVQRAGGNRWDTSSLITRLVPPRP"
     gene            complement(3753..>4399)
                     /locus_tag="PK518_14360"
     CDS             complement(3753..>4399)
                     /locus_tag="PK518_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRM50141.1"
                     /translation="VEDPIEFVHPHKNCVVTQREVGLDTDTWEAALKNTLRQAPDVIL
                     MGEIRDRETMEHAVAFAETGHLCLATLHANSANQALDRIVNFFPEERRAQLLMDLSLN
                     LKAMVSQRLLPKQDGKGRAAAVEVMLNTPLIADLIFKGDVPEIKEIMKKSRNLGMQTF
                     DQSLFDLFEANVIGYEDALRNADSVNDLRLQIKLSSQRAKTNDLSSGTEHFAIV"
BASE COUNT          667 a         1363 c         1557 g          812 t
ORIGIN      
        1 ccaccaaatc gcaacgccca accgttctcg gttgggcgtt tttttgtgga tcggatgcac
       61 ccctgcgggc tggctattgg gtgcgcgcgc ctgtcagcag gcgtttcagt cctgttcact
      121 gcgtttgatc tgcagtgcta gttcgtacag ggcatttcgc ggcgcattgg tgatgtccgc
      181 cgtcagcttc acggcggttt tggtgggtag ctcggccagc agcaggcgca gcacgcgctc
      241 ggccgcagcg tcatcgtctt gcgggacggg cgcagggtgc agcagcacca cgaactcgcc
      301 cttgctgcgc tggggtgctc cctgtagcca ggcggcaaga tcctgggccg cgtgggtggt
      361 gacttcttcg aactgtttgg tcagttcgcg tgccagtgtc acgcggcgct ctcccagcat
      421 ggccaaggcc tgtgccagtt cgagtatgcg gtgcggcgcc tccagcagca gcacgcagcg
      481 cggctcggcg gccaggcgct gtatggcgct gttgcgctcc gcgctcctgc tgggcaggaa
      541 tcccgcgaat acgaagccct ggttttcgct ggcgtgggcg atggcaccgc tcacgctcag
      601 ggcactggtc acgctgctgg cgccgggcag gggcatgcag cgcaggccgg cggcctgcac
      661 ggctgcaacc aggcgcgcgc cggggtcgct cactgcgggt gttccggcgt cgctcacata
      721 ggccacgcgc tgtccggcct gcaggcgctg gagcacggtc tgtgcggcct ccacctcgtt
      781 gtgctggtgc agcgccagca ggcgttcgcc gggtttgtcg atgccgtagg cacgcagcag
      841 tgcctgggtg tggcgcgtgt cctcgcaggc gatggtgtcg gccagttgca gcacatgcag
      901 ggcgcgcagg ctgatgtcgg ccagattgcc aatgggtgtg gccaccacgt acagggcgcc
      961 ctgcggataa tgctgggcag ccgcagcgtc gcgcgcggca tgcagggcag aggcaaagga
     1021 tgcgctcaat ggggttcctc ggcaaaaaga tcatgggtgg tgcgccaagg cacaccacgc
     1081 gtgccatagg gcaggtggct gaagaccggg cgctggcctg tctggttgcg gcgggcctgg
     1141 tgctggtcga gcgcaattat cgaaccccgg ggcgcggcgg cggcgagatt gaccttgtca
     1201 tgcgcgagcg cgacggcacg ctggtgttcg tggaggtgcg cagccgcggt gctggcgatt
     1261 ttggtggggc agcggccagc ataggcgcca ccaagcgccg gcgcatcatt ctggcggcgc
     1321 agcactatct gttgcgctgg tctgcgccgc caccatgccg tttcgatgcc gtgctcatcg
     1381 acgatggccg ggtgcagtgg ttgcgtgctg ccttcgatgc cgggtaatgg ggctggtcta
     1441 ccgtttgcta cagaaactgc aacatcggcg ggtatcattc gcggcccatg cttgagcaac
     1501 gcattcaaca gcatttcatc gacagcgccg acctgaagta ccaggctgcg caagccctca
     1561 gccagcccat cactgccgca gtgcaggcga tatgggcctg tgtgaccaat ggcgcgaagg
     1621 tgctcgcctg cggcagtggc ccatcggccg cgcaggcgca gcagctggcg gctttttgcg
     1681 tgaccggttt tgagcgcgaa cgcccggaac tggcagcgct ggccctggtg cccgacgcgc
     1741 tgtggctggc cgcggccacg acgggtgccg gtgcggacat cgcagccctg gcgcgccagg
     1801 tgcgtgctct gggccaggcc ggtgatctgc tgctggccat caccattgcc ggcaatgagc
     1861 cagccatgct ggaggcggtg caggctgccc atgagcgcga catgaccgtg gtggccctga
     1921 ctggccgcga tggcggtgcc ctggcgacct tgctgcgtga gaccgatgtg cagatctgcg
     1981 tgccgcatga ccgcccagcg cgcgtgcgcg aggtgcatgc cctggtgctg cactgccttt
     2041 gcgatggcgt ggacgcacag ttacttggag aacaggagaa ccccatatga agatgcagtc
     2101 gatgcgaacc cttggcgcct tgctggcagc ggcctccctg gtcggcagtc ttgccgcctg
     2161 tgtgccgctg gtggttggcg gcggtgccgt ggtgggcacc atgatggccg tcgataggcg
     2221 caccgcgggc acgcaggtcg aggacgaggg catagagctg cgcgcagtga accgtattcg
     2281 cgaagcaatg ggcgaacgcg cgcatgtgaa cgtgaccagc tacaaccgcc aggtcttgtt
     2341 gaccggcgag gtgcccacgg cggcagacag ccagaaggtg gagcagatcg tgctggcggt
     2401 ggataacgtg cgctcggtgg tgaacgacct gggcgtgatg gccaacacct cgctgtcgca
     2461 gcgctccaag gacactttca tcaccggcaa ggtgcgcgcc agcctggtgg acgccaagga
     2521 tctgacggcc aatgccttca aggtggtgac cgagcgcggc gtggtctatt tgatgggccg
     2581 cgtcacgcag cgcgaggcca agcgttcggc cgagatcgcg cgtggcgtgg atggtgtgca
     2641 caaggtggtg cgcgtgttcg aggtcatttc cgaggaggag ctggcgcgcg gcgtggcggc
     2701 gcaaccggcg ccagtttcac aggatccggc ggccgcgcgt tgactatcag ggtcggggtg
     2761 gcaccagcct ggtgatcagg ctggaggtgt cccagcggtt gccgcccgcg cgctgcacgt
     2821 cggcatagaa ctgatccacc agcgccgtca ccggcaggcg tgcgccattg cgtctggctt
     2881 cgtcgagcac cagtcccaga tccttgcgca tccagtccac ggcaaagccg aagtcgaagc
     2941 ggccttggac catggtcttg ccgcggttgt ccagctgcca gctttgcgcc gcgcccttgc
     3001 cgatcacgtc cagcaccagc ggcatgtcca ggcccgcctg ctgcccgaag gcaatcgcct
     3061 cggacaggcc ctgcaccagg ccggcgatgc agatctggtt gaccatcttc gcgagctggc
     3121 cggcgccgct gtcgccgatg cgcgtgcagg cgcgtgaaaa cgccatcgcc gtggggcgca
     3181 tggcatcgaa ggctgcctgg tcgccgccgc acattacggt gagctggcca ttctgcgcgc
     3241 cggcctggcc gccggagacg ggggcgtcga tgaactgcag ccccagcttc ttggcggcgg
     3301 cgtagagttc gcgcgccacc tcggccgagg ccgtggtgtg atccacgaag gttgcgcccg
     3361 gccgcatgcc ggcaaaggcg ccatcggccc ccagcaccac gctgcgcagg tcttcgtcat
     3421 tgcccacgca gcagaagacg atctcggcat cctgggccgc ctcgcgcgga gttttggcat
     3481 gtttttggcc tgtaacgccc gcaaattcag cgcaccaagc ttctgatttt gtagcggtac
     3541 ggttgtacac cgtgacctgg tgcccggccg tcgccagatg cccggccatc ggatagccca
     3601 tgacgcccag gcccagaaag gccacgcggc gcgtggggct tggttcatag ctgcgtgggt
     3661 tgatgctgct catggtggtt tgcgctccag aagaaaaaac aaggcccgcc cggcatggta
     3721 agccgggcgg gccggggtgt tgatcacgcg cttcagacga tggcgaaatg ctcggtgccc
     3781 gaagacaggt cgttggtctt ggcgcgttgg ctggacagct tgatctgcag gcgcaggtcg
     3841 ttcaccgagt cggcgttgcg cagcgcgtcc tcgtagccga tgacgttggc ctcgaacagg
     3901 tcgaacagcg actggtcgaa ggtctgcatg cccaggttgc ggctcttttt catgatctcc
     3961 ttgatctcgg ggacatcgcc cttgaagatc aggtcggcga tcaggggggt attgagcatg
     4021 acctccacgg cggcggcgcg gcccttgccg tcctgcttgg gcaacaggcg ctgcgacacc
     4081 atggccttga ggttcagcga caggtccatc agcagctgcg cgcggcgctc ctcggggaag
     4141 aagttgacga tgcgatccag cgcctggttg gcgctgttgg catgcagcgt ggccaggcac
     4201 aggtggccgg tttcggcaaa ggccacggca tgttccatgg tctcgcggtc gcggatttcg
     4261 cccatcagga tcacgtcggg cgcctggcgc agcgtgttct tcagcgcggc ctcccaggtg
     4321 tccgtgtcca ggcccacctc gcgttgcgtg accacgcagt tcttgtgcgg gtgcacgaac
     4381 tcgatcgggt cttcgacgg
//
LOCUS       DAOMTQ010000203         4393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5263_length_4393_cov_22.053020, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000203 DAOMTQ010000000
VERSION     DAOMTQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_4393_cov_22.053020"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK518_14365"
     CDS             <1..969
                     /locus_tag="PK518_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM50142.1"
                     /translation="RLLTVHGAKGLEAHSVLLLDTDAQPKKAETMAVLVDWPGEQALP
                     RRFIFLARESSPPPSAEAAMAHEKAERQREFLNTLYVAITRAKQCLVLSCVQSKNANP
                     GSWWERLAPLMQQDVQPQDAVAAASADAAFTLLELPALPPMAGGEGAQPSPATAADPD
                     SLSARQGQAMHALLEQVGGAGVPLAQARSSGWDARRLARLAREFSLPASAAQAAAAMA
                     QTILNGDGAWAWDDDALDMAVNEAPLQHQGQSLRIDRLVRLRQARADARWWVLDYKSA
                     ADPARQPALMEQLQRYRQAVQQQQPGEAVGAAFLTGEGRVVVLAAD"
     gene            complement(983..1906)
                     /locus_tag="PK518_14370"
     CDS             complement(983..1906)
                     /locus_tag="PK518_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM50143.1"
                     /translation="MDRIDQLRIFIRVAHSGSFTLAADQLGLPRPTVSLALQQLEARL
                     GARLLHRTTRRVSLTPDGAALLERAPSLVADVEELEQQFRPDAGPLAGRIRVDMPSRI
                     ARRLVAPALPAWLALHPGLQIELGSSDRSIDLVQEGVDCALRVGTPASSSLVARPVGR
                     VRLIHCASPAYLARYGVPHSPLDLPRHQAVHYAAPGSGRAAPWEWEEQGRIQQCPMDG
                     SVTVNNAETYIACCLAGLGLIQIPAFDVREHLAAGELVELLQDWPAPAMPMHLVYPHR
                     RQLSQRLQAFSQWLLPLLQQACCEDGEPPRP"
     gene            2011..2784
                     /locus_tag="PK518_14375"
     CDS             2011..2784
                     /locus_tag="PK518_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009973227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM50144.1"
                     /translation="MSTHSIQGKVVLIAGGAKNLGGLLARDLAAHGAKAVAIHYNSAA
                     SKADADATVAAVQKAGARAVALQADLTSAAAMEKLFADAVAAVGRPDIAINTVGKVLK
                     KAIADISEAEYDEMSAVNAKSAFFFLKEAGKHVNDNGKICTLVSSLLGAFTPFYASYA
                     GTKAPVEHFTRAAAKELGARGISVTAVGPGPMDTPFFYGQESADAQAYHKSAAALSPF
                     SKTGLTDIEDVVPFIRHLVSDGWWITGQTILINGGYTTK"
     gene            2797..3066
                     /locus_tag="PK518_14380"
     CDS             2797..3066
                     /locus_tag="PK518_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50145.1"
                     /translation="MHVALVMAGGLVLLGLFVLFGWLWGASSAGMALAAKWFVPAWLL
                     VAAVNMWVGVQHAGYSVREEAPILLIVFAVPAAVAAFVVWWLPRS"
     gene            3207..>4393
                     /locus_tag="PK518_14385"
     CDS             3207..>4393
                     /locus_tag="PK518_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HRM50146.1"
                     /translation="MAAEILAQAGHSVHVFDAMPSVGRKFLLAGKGGLNLTHSEPFEA
                     FVSRFGERRAQIEPLLSVFGAEQLRAWAQGLGVDTFVGTSGRVFPTDMKAAPLLRAWL
                     ARLRAAGVVLHMRHRWLGWDADGALCFASAAGQQRVAARATVLALGGASWPRLGSDAA
                     WVPLLAARGVQIAPLQPANCGFDVPRVDAPVGHLFDISRREFFRELLGRGQASAVGWT
                     PFFAERFAGQPFKSVAIAFTDSQGRQFARRGEFVATATGVEGSLVYAASGLLRDEITA
                     HGHATFHLDLLPDHSPERVLAEVRHPRGARSLTSHLKGRLGLDGIKAAILYEQLGKVG
                     MQDAQLLAQAIKRLPITVLAMRPVQEAISTAGGVSFEALDAHLMLRAMPGVFCAGEML
                     DWEA"
BASE COUNT          647 a         1468 c         1578 g          700 t
ORIGIN      
        1 cgcctgctca ccgtgcacgg cgccaagggg ctggaggccc acagcgtgct gctgctcgac
       61 accgacgccc aaccaaaaaa agccgagacc atggccgtgt tggtggactg gccgggcgag
      121 caggcgctgc cgcggcgctt catatttctg gcgcgcgaat ccagcccgcc acccagcgca
      181 gaggccgcca tggcgcacga gaaggccgag cgccagcgtg agtttttgaa caccttgtac
      241 gtcgccatca cacgcgccaa gcaatgcctg gtgctgtcct gcgtgcaatc gaaaaacgcc
      301 aaccccggca gctggtggga gcgcctggcg cccttgatgc agcaagacgt gcaaccgcag
      361 gacgccgtcg ccgctgccag cgcggacgcc gccttcaccc tgctggagct gccggcgcta
      421 ccccctatgg cagggggtga gggcgcccag cccagcccgg cgacggcagc agacccggac
      481 agcctgagcg cccgccaggg ccaggccatg cacgcgctgc tcgagcaggt gggcggggcc
      541 ggcgtcccgt tggcgcaggc ccgcagcagc ggctgggacg cgcgccgcct ggcgcgcctg
      601 gcgcgtgaat tttctctgcc ggccagcgcc gcccaggccg ccgccgccat ggcgcagacc
      661 atcttgaacg gtgacggcgc ctgggcctgg gacgacgatg ccttggacat ggcggtcaac
      721 gaagcgccgc tgcagcacca gggccaaagc ctgcgcatcg accgcctggt gcgcctgcgc
      781 caggcccgcg ccgacgcgcg ctggtgggtg ctggactaca agagcgccgc cgaccccgcg
      841 cgccaaccgg cgctgatgga gcagctgcaa cgctaccgcc aggcggtcca gcagcagcag
      901 cccggcgagg ccgtgggcgc ggcgttcctg acgggcgagg gcagggtggt ggtactggcg
      961 gctgattgag ccgagagctt cgtcaggggc gaggcggctc gccatcctcg caacaggcct
     1021 gctgcagcag gggtagcagc cattgactga aggcctgcag ccgctgcgac agctggcgcc
     1081 ggtgcgggta gaccaggtgc atgggcatgg cgggcgcggg ccagtcctgc agcagctcga
     1141 ccagctcgcc ggcggccagg tgctcgcgca cgtcgaaggc cggaatctgg atcagcccca
     1201 gcccggccag gcagcaggcg atataggtct cggcgttgtt cacggtcacg ctgccgtcca
     1261 tggggcattg ttggatgcgg ccttgctcct cccattccca gggcgcggcg cggccgctgc
     1321 cgggcgcggc gtagtgcacg gcctggtgcc gcggcaggtc cagcgggctg tgcggtaccc
     1381 cgtagcgcgc cagataggcc gggctggcgc agtggatcag ccgcacccgc cccacgggcc
     1441 gggccaccag gctgctgctg gccggcgtgc ccacgcgcag cgcgcagtcc acgccttctt
     1501 gcaccaggtc gatgctgcgg tcgctgctgc ccagctcgat ctgcaggccg gggtgcaggg
     1561 ccagccatgc gggcagcgcc ggcgccacca ggcggcgggc gatgcggctg ggcatgtcca
     1621 cgcggatgcg cccggccagc ggcccggcgt cggggcgaaa ctgctgctcc agctcctcca
     1681 catcggccac cagcgagggc gcgcgctcca gcagggctgc gccgtcgggc gtcaggctga
     1741 cgcggcgcgt ggtgcggtgc agcaggcggg cgcccaggcg cgcttccagc tgctgcagcg
     1801 ccagcgacac ggtggggcgc ggcaggccca gctggtcggc cgccagggta aagctgccgc
     1861 tgtgcgctac gcggatgaag atgcgcagct ggtcaatgcg atccatggag ggtgattggt
     1921 agtgatttat ggatggagca tctatttttc agcatttatt tgtgttttgc ttatcaataa
     1981 agtggcgctt ttacccacaa ggagccatcc atgtccacac acagcatcca aggcaaagtc
     2041 gttctgatcg ccggtggcgc caaaaacctc ggtggcctgc tggcgcgtga cctggcggcg
     2101 catggcgcca aggccgtggc cattcactac aacagtgccg ccagcaaggc cgacgccgac
     2161 gccaccgtgg cggcggtgca aaaggccggt gcacgggccg tggccctgca ggccgacctg
     2221 accagcgccg ccgccatgga aaagctgttt gccgacgccg tggccgccgt cggccgcccg
     2281 gacattgcca tcaacaccgt gggcaaggtg ctcaagaagg cgattgccga catctccgag
     2341 gccgagtacg acgagatgag tgcggtcaac gccaagtcgg cctttttctt cctcaaagaa
     2401 gcgggcaagc atgtcaacga caacggcaag atttgcaccc tggtgagctc gctgctgggc
     2461 gcattcacgc cgttctatgc gtcctacgcg ggcaccaagg cgccggtgga gcatttcacg
     2521 cgcgcggctg ccaaggagtt gggcgcacgc ggcatctcgg tcacggccgt gggccccggc
     2581 cccatggaca cgcccttctt ctacggccag gaaagcgccg acgcccaggc ctaccacaag
     2641 agtgccgccg cgctatcgcc cttcagcaag accggcctga ccgacatcga agacgtggtg
     2701 cccttcatcc gccacctggt cagcgatggc tggtggatca cgggccagac gattctgatc
     2761 aacggcggct ataccaccaa gtaacggggg gaagccatgc atgtagcgct tgtgatggcc
     2821 ggcggcctgg tgctgctggg cctgttcgtg ctgtttggct ggctgtgggg cgccagcagc
     2881 gctggcatgg cgctggcggc caagtggttt gtgccggcct ggctgctggt ggcggcggtg
     2941 aacatgtggg tgggcgtgca gcacgcgggt tacagcgtgc gcgaggaggc gcccattttg
     3001 ctcatcgtct ttgccgtgcc ggcggcggtg gctgcttttg tggtgtggtg gctgccgcgt
     3061 agctgaagag ctgcgggcgg tgcggccgac tgcgtggatg cagggccgca aggtgggatg
     3121 atcggcgcat gtcttatgtg aacgattctt ccagcgcgcc cctcgcgtgc gacgccgcca
     3181 tcatcggcgg cggcccggcc gggttgatgg ctgccgaaat actggcccag gcgggccatt
     3241 ctgtgcatgt gttcgacgcc atgccctctg tcgggcgcaa gtttttgctg gccggcaagg
     3301 gcgggctgaa tctgacccac tccgagccct tcgaggcctt tgtgtcgcgc ttcggcgagc
     3361 gccgcgcaca gatcgagccg ctgctgagcg tctttggcgc cgagcagctg cgcgcctggg
     3421 cgcagggctt gggcgtcgat acgtttgtcg gcacctcggg gcgcgtcttt cccaccgaca
     3481 tgaaggccgc gccgctgctc cgcgcctggc tggcgcggct gcgtgcagct ggcgtggtgc
     3541 tgcacatgcg ccaccgctgg ctgggttggg atgccgacgg cgccttgtgc tttgccagcg
     3601 ccgccgggca gcagcgcgtg gccgcgcgcg ccacggtgct ggcgctggga ggcgccagct
     3661 ggccgcgtct gggctccgac gctgcctggg tgccgctgct ggcggccagg ggagtgcaga
     3721 ttgcgccatt gcagccggcc aactgcggct ttgacgtgcc gcgcgtggac gcgcccgtgg
     3781 gccacctttt tgacatttcg cggcgcgagt tctttcgcga gctgctgggg cgcggccagg
     3841 catccgccgt gggctggaca ccgttttttg ccgagcgctt tgcgggccag ccgttcaagt
     3901 cggtggccat tgccttcacc gacagccagg gccgtcagtt tgcgcggcgc ggcgagttcg
     3961 tcgccaccgc cacgggggtg gagggcagcc tggtctatgc cgcctccggc ctgctgcgcg
     4021 atgaaatcac ggcccacggc cacgccacct tccatctgga cctgctgccc gaccacagcc
     4081 ccgaacgggt gctggccgag gtgcgccatc cgcgtggtgc gcgcagcctg acaagccacc
     4141 tcaaggggcg gctggggctc gatggcatca aggccgccat cctctacgaa cagctgggca
     4201 aggtcggcat gcaggatgcg cagctgctgg cacaggccat caaacggctg cccatcaccg
     4261 tgctggccat gcgcccggtg caggaggcga tcagcaccgc cggcggcgtg tccttcgagg
     4321 cgctggatgc gcatctcatg ctgcgggcca tgcccggggt gttctgcgcc ggcgagatgc
     4381 tggactggga ggc
//
LOCUS       DAOMTQ010000204         4381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5291_length_4381_cov_28.305825, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000204 DAOMTQ010000000
VERSION     DAOMTQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_4381_cov_28.305825"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PK518_14390"
     CDS             complement(<1..875)
                     /locus_tag="PK518_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM50147.1"
                     /translation="MAMDRINWDNLRLFLAVVRAQSAQEAARRLELDHSTITRRLHRL
                     EKELGARLFERTPAGHLLTPVGQRLLEHVERMESSMVLVGEDIGGDSHMLTGHVRLGA
                     TEGFGSFFLAPQLSHFCERHPGIQVELLIVPRFINLSQREADLAVNIERPHRTGELCS
                     KLTDYRLRLYASRRYVSEHGPIRRLQDLAAHRFFGYVEELVFSQELHYLSDIVPDVSS
                     SLRSTSVVAQYHAVREGRGLAVLPCFMAEPCQELIAVLPDEVELRRTFWLAAPADRRE
                     LARVRALWDYLRELV"
     gene            1014..2228
                     /locus_tag="PK518_14395"
     CDS             1014..2228
                     /locus_tag="PK518_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM50148.1"
                     /translation="MQPLRHSIALAALAFCAGAQAQISDDVVKIGILTDMAGPYSGMG
                     GAGSVVAARMAIDDCLKAECKGMKIEVVSADHQNKADIAATKAREWLDRDKVDALADL
                     TNSAGALAVQKLIKEKGGIAMYSGPATTRLTEDDCAENGFHWMFDTYSSAAGAAVALT
                     KAGDKSWFFVTVDYAFGHSLEKDASDMVKANGGTVVGSVRHPLNASDFSSFILQAQNS
                     KAQVIGLANGAQDTVNAIKAAREFGITGAKGQKVAALLMFLTDVHSLGLRQAQGLMFS
                     EGFYWDMDDKTRAFSARFEKLHKGFKPTMVQAGVYSSVLHYLRSVAAAKTDDWKVVAQ
                     KMRELPIDDPVMRNASIRPDGRVIHDMYLFQVKTPAESKGAWDYYKTVSTIPANAAFK
                     PLAQSTCPLVKK"
     gene            2244..3749
                     /locus_tag="PK518_14400"
     CDS             2244..3749
                     /locus_tag="PK518_14400"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRM50149.1"
                     /translation="MSTPTTIHHFIGGKHYPSSTNQWRDVTNPATQEVVARVPFATRE
                     EVELAVANAHQAFQSWRSSSLGARMRIMLKLQHLIRENTAELAQLITREHGKTLPDAE
                     GEVARGLEVVEHACSITTLQLGEIAENAATGVDVYNLLQPLGVGAGITAFNFPVMLPC
                     FMFPMAIACGNTFILKPSEQDPSSTMRLVELAHEAGVPPGVLNVIHGGPEVADMLCDH
                     PDIKALSFIGSTHVGTHIYRRASEAGKRVQSMMGAKNHCVVMPDAPKEHALNNLLGSA
                     FGAAGQRCMANSVAVFVGAARDWLPDLVAKSKALKVGPGSDRSADLGPLVNPRAKERV
                     LGLIDSGVAQGAKLLLDGRSLTVPGYEKGNFVGPTVFADVTETMDIYSQEIFGPVLNV
                     VCVDTLEDAIAFINRNPNGNGTSIFTSSGWAARKYQHDINVGQVGINVPIPVPVAYFS
                     FTGSRASKLGDLGPNGKQAVLFWTQTKTVTARWYEDHGSASDGVNTTISMK"
     gene            complement(3844..>4381)
                     /locus_tag="PK518_14405"
     CDS             complement(3844..>4381)
                     /locus_tag="PK518_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM50150.1"
                     /translation="GTSNRHGAASVTQFEVMPMPPEQENKPLVWPYWPLKLRTSSSHD
                     EGCVREFAISTKEFIGQKGKVTGLKTVQVEFKNGQFTEVPGTEKEYKADLVLLAMGFT
                     SPVAAVLDAFGVEKDARGNARASVDFIGGYATSVPKVFAAGDMRRGQSLVVWAIREGR
                     QAARAVDEFLMGRSDLPR"
BASE COUNT          798 a         1486 c         1391 g          706 t
ORIGIN      
        1 tccaccagct cgcgcaggta gtcccacagc gcgcgcaccc gcgccagctc gcggcggtcc
       61 gctggcgctg caagccagaa ggtgcgccgc agctccacct cgtcgggcag cacggcgatc
      121 agctcctggc agggctcggc catgaagcag ggcagcacgg ccaggccgcg gccctcgcgc
      181 accgcgtggt actgcgccac cacgctggtg ctgcgcaggg agctggaaac atcggggacg
      241 atgtcgctca ggtaatgcag ctcctggctg aagaccagct cctccacata gccgaaaaat
      301 cggtgcgcgg ccagatcctg cagtcggcgg atgggcccat gctcgcttac ataacgccgg
      361 ctggcataca gccgcaggcg gtaatcggtg agcttgctgc acagctcgcc cgtgcggtgc
      421 ggacgctcga tgttcacggc cagatcagcc tcgcgctgcg agaggttgat gaagcgcggc
      481 acgatcagca gctccacctg tatgcccgga tggcgctcgc agaaatggct cagctgcggc
      541 gccaggaaga agctgccgaa accctcggtc gcacccaggc gcacatggcc ggtgagcatg
      601 tggctgtcgc cacctatgtc ctcgcccacc agcaccatgg agctttccat gcgctccaca
      661 tgctccagca ggcgctggcc cacgggcgtg agcaggtgac cagccggcgt gcgctcgaac
      721 agccgtgcgc ccagctcctt ctccagccgg tgcaggcggc gcgtgatggt ggaatggtcc
      781 agctccaggc gccgggcagc ctcctgcgcc gactgggcac gcaccacggc gaggaacagg
      841 cgcaggttgt cccagttgat gcgatccatg gccatgcttg cgggaactgt agtggtgcaa
      901 atctgcattg agcatatgcc caaatgccct atccctttgc ataactgttg gcttagagtg
      961 gtctgcgccg tggccttggc ccggacacgc gatcgacaac ccaggagaca tgcatgcaac
     1021 ccctaagaca ttccattgcc ctcgccgcgc tggcattctg cgccggtgca caggcgcaga
     1081 tttccgacga cgtggtgaag atcggcatcc tcaccgacat ggccggcccc tactcgggca
     1141 tggggggcgc gggctcggtg gtggcggcca ggatggccat cgacgactgc ctgaaggccg
     1201 aatgcaaggg catgaagatc gaggtggtga gcgccgacca ccagaacaag gccgacatcg
     1261 ccgccaccaa ggcgcgcgaa tggctggacc gcgacaaggt cgatgcgctg gcagacctga
     1321 ccaactccgc cggcgccctg gcggtgcaga agctgatcaa ggaaaagggc ggcatcgcca
     1381 tgtacagcgg accggccacc acgcgcctga ccgaggacga ctgtgccgag aatggctttc
     1441 actggatgtt cgacacctat tcctcggccg ccggcgccgc cgtggcgctg accaaggccg
     1501 gcgacaagag ctggttcttc gtcaccgtgg actacgcctt cggccattcg ctggagaagg
     1561 atgcgtctga catggtcaag gccaacggcg gcaccgtggt gggcagcgtg cgccacccgc
     1621 tgaacgccag cgatttctcg tccttcatcc tgcaggcgca gaactccaag gcccaggtca
     1681 taggcctggc caacggcgcg caggacaccg tgaacgccat caaggccgcg cgcgagttcg
     1741 gcattaccgg cgccaagggc cagaaggtgg ccgccctgct gatgttcctg accgacgtgc
     1801 attcgctcgg cctgcgccag gcccaggggc tgatgttctc cgagggcttc tactgggaca
     1861 tggacgacaa gacgcgcgcc ttctcggccc gcttcgagaa gctgcacaag ggcttcaagc
     1921 cgaccatggt gcaggcgggg gtgtattcca gcgtgctgca ttacctgagg tccgtggccg
     1981 ccgccaagac cgacgactgg aaggtggtgg cgcagaagat gcgcgagctg cccatcgacg
     2041 acccggtgat gcgcaacgcc agcattcgcc ccgacggccg cgtgatccac gacatgtatc
     2101 tgttccaggt caagaccccg gccgagtcca agggcgcctg ggactactac aagaccgtga
     2161 gcaccattcc cgccaacgcc gccttcaagc cgctggccca gtccacctgc cccctcgtga
     2221 agaaataacc ccaggagaga gtcatgagca cccccaccac catccaccat ttcattggtg
     2281 gcaagcatta cccgtccagc accaaccagt ggcgcgacgt gaccaacccc gcgacgcagg
     2341 aggtcgtggc gcgcgtgccc tttgccacgc gcgaagaagt ggagctggcc gtcgccaacg
     2401 cgcaccaggc cttccagagc tggcgcagca gctcgctggg cgcgcgcatg cgcatcatgc
     2461 tcaagctgca gcacctgata cgcgaaaaca ccgccgagct ggcgcagctg atcacgcgcg
     2521 agcatggcaa gaccctgccc gacgccgagg gcgaggtggc gcgcggcctg gaggtggtgg
     2581 agcatgcctg ctccatcacc acgctgcagc tgggcgaaat tgccgagaac gccgccaccg
     2641 gcgtggacgt gtacaacctg ctgcagcccc tgggcgtggg cgcgggcatc acggccttca
     2701 acttcccggt gatgctgccc tgcttcatgt tccccatggc gattgcctgc ggcaacacct
     2761 tcatcctcaa gccctccgag caggatccca gctcgaccat gcgcctggtg gagctggcgc
     2821 atgaggcggg cgtgccgccc ggcgtgctca acgtgatcca cggcggcccc gaggtggccg
     2881 acatgctgtg cgaccacccg gacatcaagg ccctgtcctt catcggctcc acccatgtgg
     2941 gcacgcatat ctaccgccgc gccagcgagg ccggcaagcg cgtgcagtcc atgatgggcg
     3001 ccaagaacca ttgcgtggtc atgcccgatg cgcccaagga gcatgcgctg aacaacctgc
     3061 tgggctcggc ctttggcgcc gcgggccagc gctgcatggc caactcggtg gccgtgttcg
     3121 tgggcgccgc gcgcgactgg ctgcccgatc tggtggccaa gtccaaggcc ctgaaggtcg
     3181 gccccggcag cgaccgcagc gccgacctgg ggcccctggt gaacccacgc gccaaggagc
     3241 gcgtgctcgg cctgatcgat tccggcgtcg cccagggtgc caagctgctg ctggacggcc
     3301 gcagtctgac cgtgcccggc tacgagaagg gcaacttcgt cggccccacg gtgtttgccg
     3361 acgtgaccga gaccatggac atctacagcc aggagatctt cggccccgtg ctcaacgtgg
     3421 tctgcgtgga tacgctggaa gacgccatcg ccttcatcaa ccgcaacccc aacggcaacg
     3481 gcacctccat cttcaccagc agcggctggg cggcgcgcaa gtaccagcac gacatcaacg
     3541 tgggccaggt gggcatcaac gtgccgatcc cggtgccggt ggcctacttc agcttcaccg
     3601 gctcacgcgc ctccaagctc ggcgaccttg ggcccaacgg caagcaggcc gtcctgttct
     3661 ggacccagac caagaccgtg acggcccgct ggtatgagga tcacggcagc gccagcgatg
     3721 gcgtgaatac caccatctcg atgaagtagg accgcgccag gcggccagga agaaagcacc
     3781 tatagctata atttaaatag ctttaggcgc ttgtcacata aggcctattg gccattttta
     3841 ttcttaacgc ggtaggtcgc tgcgccccat caggaactca tcgaccgcgc gcgccgcctg
     3901 gcgcccttcg cggatggccc agaccaccag cgactgcccc cggcgcatgt cgccggcggc
     3961 aaacaccttc ggaacactcg tggcgtagcc accgatgaaa tccacgctgg cgcgggcgtt
     4021 gccgcgtgcg tctttctcga cgccaaaggc atcgagtacg gcggccacgg ggctggtgaa
     4081 gcccatggcc agcagcacca ggtcggcctt gtattctttt tcggtgccgg gcacctcggt
     4141 gaactggccg ttcttgaact caacctgcac ggttttcagg cccgtgacct tgcctttctg
     4201 cccgatgaat tccttggtgg agatggcgaa ctcgcgcacg cagccctcgt cgtggctgga
     4261 gctggtgcgc aatttcagcg gccagtaggg ccagaccagg ggcttgtttt cctgctcggg
     4321 cggcatgggc atgacctcga actgcgtcac gctggccgcg ccgtggcggt tgctggtgcc
     4381 c
//
LOCUS       DAOMTQ010000205         4376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5301_length_4376_cov_27.588521, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000205 DAOMTQ010000000
VERSION     DAOMTQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5301_length_4376_cov_27.588521"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /gene="fabA"
                     /locus_tag="PK518_14410"
     CDS             <1..317
                     /gene="fabA"
                     /locus_tag="PK518_14410"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HRM50151.1"
                     /translation="QGDPVMPGCLGLDAMWQLIGFYLTWLRLPGRGRALGAGEVKFTG
                     EVGPDVKLVTYEIDIKRVIKRKLNMAIGDARLLADGKEIYVANDLRVGLFLREEGAKE
                     AA"
     gene            314..1537
                     /gene="fabB"
                     /locus_tag="PK518_14415"
     CDS             314..1537
                     /gene="fabB"
                     /locus_tag="PK518_14415"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HRM50152.1"
                     /translation="MSKKRVVITGAGIVSCIGNDLDTVEASLRQGKSGIRAVEKFTEL
                     GLRSQVAGRPEIDIEARIDRKQLRFMGDAAAYAQIALEDAIKQAGLSPEQVSHPRTGL
                     IMGSGGGSPANQIEAADTLREKGIRRVGPYQVTRCMSSTVSACLATNFKVKGINYSIT
                     SACSTSAHCIGAAAQQIAWGMQDVMFAGGGEELSWGMALLFDGMGAMSAKYNATPDKA
                     SRAYDAHRDGFVIGGGGGAVVLESLEHALARGATILAEVVGFGATSDGEDMVAPSGDG
                     AVACMRQAMEGLAGPIDYINTHGTSTPVGDMQEVRAMRAVFGDQVPPFSSTKSLTGHS
                     LGATGVQEAIYCLIMMNKGFIAGSVNVETPDPELDGMPLVTATRDAQLTQVLSNSFGF
                     GGTNASLVLRRWQGS"
     gene            1699..2934
                     /locus_tag="PK518_14420"
     CDS             1699..2934
                     /locus_tag="PK518_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HRM50153.1"
                     /translation="MTPHTALRLHVPEPTGRPGCKTDFSYLSVSPAGSVRRPPPDTAP
                     ADTADLATSLVRVLDDDGKAQGPWAPAMHPERMRRGLRAMMKTRIFDARMLLAQRQKK
                     LSFYMQCLGEEAIAVAHSMALQDGDMCFPTYRQQGLLLARDDVSMAEMICQLMSNEAD
                     PIKGRQLPVMYSYKRAGFFSISGNLGTQVPQAVGWAMASAIKGDTKIASAWIGDGSTA
                     ESDFHTALTFAHVYRAPVIINVVNNQWAISTFQAIAGGEGTTFAQRGVGVGIASLRVD
                     GNDFLAVYAASQWAAERARSNCGPTLIEWETYRAGPHSTSDDPSKYRPADDWQRFPLG
                     DPIERLKKHLIVIGEWSEGQHEAAHKELEAEVMAAQKEAERHGSLLDGRVPSAATMFD
                     DVYKDMPEHLRRQRQQMGV"
     gene            2938..3978
                     /locus_tag="PK518_14425"
     CDS             2938..3978
                     /locus_tag="PK518_14425"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRM50154.1"
                     /translation="MAEHNQVQGGVPMTMIQALRSGLDVMMGRDDNVIVYGEDVGYFG
                     GVFRVTEGLQAKYGKTRCFDAPINESGIVATAIGMAAYGLKPVVEIQFADYFYPATDQ
                     IVSEAARLRHRSAGDFSAPMVIRMPCGGGIYGGQTHSQSPEAFFTHVCGLRTVMPSNP
                     YDAKGLLISSIECEDPVIFLEPKRLYNGPFDGHHDKPVVPWSKHDMGRVPEGYYNVPL
                     DKAVVFRPGKAVTVLAYGTMVWVAEAAAREAGVDAEIIDLRSLWPLDLDTILESVKKT
                     GRCVVVHEATRTSGFGAELTALVQEHCFYHLEAPIERVTGWDTPYPHAQEWAYFPGPA
                     RVAEALKRAVEA"
     gene            3983..>4376
                     /locus_tag="PK518_14430"
     CDS             3983..>4376
                     /locus_tag="PK518_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRM50155.1"
                     /translation="MGIYVIRVPDIGEGIAEVELVTWNVQPGDSVTEDQHLADVMTDK
                     ATVEVPSPVHGKVISLGGAVGQVMAVGAELVRLEVEGEGNLKENSAPTPTSKAQAAPV
                     SVAVPAPVATPVAPPASAPAPKAAPARAA"
BASE COUNT          765 a         1477 c         1460 g          674 t
ORIGIN      
        1 tccagggcga cccggtcatg cccggctgcc tggggctgga tgccatgtgg cagctgatcg
       61 gcttttacct gacctggctg cgcctgcccg gccgtggccg cgccctgggt gccggcgagg
      121 tcaagttcac cggcgaggtc ggccccgacg tgaagctggt gacctacgag atcgacatca
      181 agcgcgtcat caagcgcaag ctgaacatgg ccataggcga tgcgcgcctg ctcgccgacg
      241 gcaaggaaat ctacgtcgcc aacgacctgc gcgtggggct gttcctgcgc gaggaagggg
      301 caaaggaagc agcatgagca agaagcgcgt cgtcatcacg ggtgcaggca tcgtgtcctg
      361 cattggcaac gacctggaca cggtcgaggc ctcgctgcgc cagggcaaga gcggcatccg
      421 cgcggtggag aagttcaccg agctgggcct gcgcagccag gtggccggcc ggcccgagat
      481 cgacatcgag gcgcgcatag accgcaagca attgcgcttc atgggcgacg ccgcggccta
      541 cgcgcagatc gcgctggaag atgcgatcaa acaggccgga ttgagccccg aacaggtcag
      601 ccacccgcgc accggcctga tcatgggttc gggcggcggc tcgccggcca accagatcga
      661 ggccgccgac acgctgcgcg agaagggcat tcgccgcgtc ggcccgtacc aggtcacgcg
      721 ctgcatgagc agcacggtgt cggcctgcct cgcgaccaac ttcaaggtca agggcatcaa
      781 ctactccatc acctcggcct gctcgacgtc ggcgcactgc ataggcgcgg cggcgcagca
      841 gatcgcctgg ggcatgcagg acgtgatgtt tgccggcggc ggcgaggaat tgagttgggg
      901 catggcgctg ctgttcgacg gcatgggcgc catgtcggcc aagtacaacg ccacgcccga
      961 caaggcgtcc cgcgcctatg acgcccatcg cgacggcttt gtgatcggcg gcggcggcgg
     1021 cgccgtggtg ctggagagcc tggaacatgc cctggcgcgc ggcgccacca tcctggccga
     1081 ggtcgtgggc tttggtgcga cgagcgacgg cgaggacatg gtcgcgccgt ccggcgacgg
     1141 cgccgtggcc tgcatgcgcc aggccatgga ggggctggcc ggccccatcg actacatcaa
     1201 cacccacggc acctccacgc cggtgggcga catgcaggag gtgcgcgcca tgcgcgccgt
     1261 gtttggcgac caggtgccgc cgttttccag caccaagtcg ctgaccggcc actcgctggg
     1321 cgccaccggc gtgcaggagg cgatctactg cctgatcatg atgaacaagg gtttcatcgc
     1381 cggctcggtc aacgtcgaga cgccggaccc tgagctcgac ggcatgccgc tggtcaccgc
     1441 cacgcgcgat gcgcagctga cccaggtgct gtccaacagt ttcggctttg gcggcaccaa
     1501 cgccagcctg gtgttgcgcc gctggcaggg cagctgattt ttgccgcccc ggtaaaaacc
     1561 cgggggtctg gcgacaactc caccacctag aatctgcggt tgcgcttttg gcgcttcagg
     1621 gacagggcgc cacgatctgg cgccctgttc tgttcctaac gctgtgcccg acacaatttg
     1681 gcacaaggag aatagtggat gaccccgcac acagccctac ggctgcatgt gcccgagcct
     1741 actggcaggc cgggctgcaa gaccgacttt tcctacctct ccgtctcgcc cgcaggcagc
     1801 gtgcgcagac ccccacccga taccgccccc gcagacaccg ccgacctggc caccagcctg
     1861 gtgcgcgtgc tcgatgacga tggcaaggcg caaggcccct gggccccggc catgcacccc
     1921 gaacgcatgc gccgcggcct gcgcgcgatg atgaagacgc gcatcttcga cgcgcgcatg
     1981 ctgcttgccc agcggcagaa aaagctctcc ttctacatgc agtgcctggg tgaggaggcg
     2041 atcgccgtgg cgcattccat ggcgctgcag gacggggaca tgtgctttcc cacctaccgc
     2101 cagcaggggc tcttgctggc gcgtgacgat gtctcgatgg ccgagatgat ctgccagctg
     2161 atgagcaacg aggccgatcc catcaagggc cgccagctgc cggtgatgta ctcgtacaag
     2221 cgcgccggtt tcttcagcat ctcgggcaac ctcggcaccc aggtgccgca ggccgtgggc
     2281 tgggccatgg cgtcggccat caagggcgac acgaagattg cgtcggcctg gatcggcgac
     2341 ggctccacgg ccgagagcga ctttcacacc gcgctcacct tcgcccatgt ctaccgcgcg
     2401 ccggtgatca tcaacgtggt caacaaccag tgggccatct ccaccttcca ggccattgcc
     2461 ggcggcgagg gcaccacctt tgcccagcgc ggcgtgggcg tgggcattgc ctcgctgcgc
     2521 gtcgatggca acgacttcct ggccgtctat gcggcctcgc aatgggcggc cgagcgcgcc
     2581 cgcagcaact gcggcccgac cctgatcgag tgggaaacct accgcgccgg cccgcactcc
     2641 acgtcggacg acccgagcaa ataccgcccc gccgacgact ggcagcgctt cccgctgggc
     2701 gacccgatag agcgcctgaa gaaacacctg atcgtcatcg gcgaatggag cgaggggcag
     2761 cacgaggccg cgcacaagga gctcgaagcc gaggtcatgg ccgcgcagaa agaggccgag
     2821 cgccacggct cgctgctcga tggccgcgtg cccagcgccg cgaccatgtt cgacgatgtg
     2881 tacaaggaca tgccggagca cctgcgccgg cagcgccagc agatgggggt ctaagccatg
     2941 gcagaacaca accaagtcca gggcggcgtc cccatgacca tgatccaggc gctgcgctcg
     3001 ggtctggacg tgatgatggg tcgcgacgac aacgtcatcg tctacggcga agacgtgggt
     3061 tacttcggcg gcgtgttccg tgtcaccgag ggcctgcagg ccaagtatgg caagacgcgc
     3121 tgctttgacg cgccgatcaa cgaatcgggc atcgtcgcca cggccatcgg catggcggcc
     3181 tacggattga agcccgtggt cgagatccag ttcgccgact acttctaccc ggccaccgac
     3241 cagatcgtct ccgaggcggc gcgcctgcgc caccgctcgg cgggcgactt ttccgccccc
     3301 atggtgatcc gcatgccctg cggcggcggc atctatggcg ggcagacgca cagccagagc
     3361 cccgaggcct tcttcaccca tgtctgcggc ctgcgcacgg tcatgccgag caacccgtat
     3421 gacgccaagg gcctcttgat ctcgtccatc gagtgcgaag acccggtgat cttcctggag
     3481 cccaagcgcc tgtacaacgg ccccttcgac ggccaccacg acaaacccgt cgtgccctgg
     3541 tccaagcacg acatgggccg cgtgcccgag ggctactaca acgtgccgct ggacaaggcc
     3601 gtggtgttcc gccccggcaa ggcggtgacg gtgctggcct acggcaccat ggtctgggtg
     3661 gccgaggcgg cagcgcgcga ggccggcgtg gacgccgaga tcatcgacct gcgcagcctc
     3721 tggccgctgg atctggacac cattctggag tcggtgaaga agaccggccg ctgcgtcgtg
     3781 gtgcacgagg ccacgcgcac cagcggcttt ggcgccgagc tgaccgcgct ggtgcaggag
     3841 cattgcttct atcacctcga agcccccata gaacgcgtga cgggttggga caccccctat
     3901 ccgcacgccc aggaatgggc gtatttcccc ggcccggcgc gcgtcgccga ggccctgaag
     3961 cgcgcagtgg aggcatgaca tcatgggtat ttatgtaatt cgcgtgcccg acattggcga
     4021 gggcatagcc gaggtggagc tggtgacctg gaatgtgcag ccgggcgaca gcgtgacaga
     4081 ggatcagcat ctggccgacg tgatgaccga caaggccacc gtcgaggtgc cgtcgcccgt
     4141 gcacggcaag gtgatttctc tgggcggcgc cgtgggccag gtgatggccg tgggcgccga
     4201 actggtgcgc ctggaggtcg agggcgaggg caatctaaag gaaaattcgg ctccaacgcc
     4261 caccagtaaa gcgcaagcag ctcctgtttc agtagctgta cctgcgccag tggcgacccc
     4321 ggtggcgccg cctgccagcg cccccgcgcc caaggccgcg cccgcccggg ccgcac
//
LOCUS       DAOMTQ010000206         4347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5341_length_4347_cov_23.565238, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000206 DAOMTQ010000000
VERSION     DAOMTQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5341_length_4347_cov_23.565238"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PK518_14435"
     CDS             <1..1172
                     /locus_tag="PK518_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRM50156.1"
                     /translation="LRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLV
                     AFGAATGVMPLDMPESVLVRFKGKLQPGVTLRDLVHAIPLYAIKAGLLTVAKAGKINV
                     FSGKILEIEGLPDLKVEQAFELSDASAERSAAGCTIKLNPEPIKEYLTSNIVLMKNMI
                     ADGYADAKTLARRIEKVEAWLAKPDLLEADKDAEYAAVIEIDMNDIKEPIVCCPNDPD
                     DAKFLSEVAGTKIDEAFIGSCMTNIGHFRAAAKLLGGQRDIPTKLWVAPPTKMDESEL
                     IKEGHYAAFGTAGARTEMPGCSLCMGNQAQVREGATVISTSTRNFPNRLGKNTNVFLG
                     SAELTAIASRLGRLPTPAEYLAEIGVVDANKDSIYRYMNFDQIEEYAEVAKGVTA"
     gene            complement(1284..2108)
                     /locus_tag="PK518_14440"
     CDS             complement(1284..2108)
                     /locus_tag="PK518_14440"
                     /inference="COORDINATES: protein motif:HMM:NF022681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HRM50157.1"
                     /translation="MKPVACRYAVLQFSPFRETGEFANAGVILLCPETGYFGFKLQTQ
                     RAKRITDFFDELPREIYLRAIKSMQDELQRVAQRVADTPAQGRSDALRHLFDALIHPR
                     EAMVRFGPARAVLTPDPATELQRQFEHCVERRFITPEYVEQTMEKRIKYLLSSLPLAA
                     PFARQRVGDEVFHASFPLVQQQGDVLTKIIKPLRLNQEAPVDIYEHGDAWLQKVKRLR
                     GRNLLPESVLFAIKPPAPDHTQRHAAYLDICSDLHSLAVQTVPDHEEQRIADFAQH"
     gene            complement(2204..2836)
                     /locus_tag="PK518_14445"
     CDS             complement(2204..2836)
                     /locus_tag="PK518_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50158.1"
                     /translation="MSTAMIPIVEVIGPAQQGLSRPLLCVGADDQRYYVKGQQTNRAS
                     LWSEWICAHLAQALGLPLPPFSLVQLDPALVRELPKDLQAVGSLPAFGSREVVHTVWL
                     ELGIDHKVAPELQRKVLAFDWWVRNTDRLRGNTNLLWDTAHQSLVVIDHNLAFGNNFD
                     AAEFIDHHVFADQWPALTHDLVEQAQHAQWLHGAMGAAATAFATAPEEWL"
     gene            complement(2833..>4347)
                     /locus_tag="PK518_14450"
     CDS             complement(2833..>4347)
                     /locus_tag="PK518_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRM50159.1"
                     /translation="MNLRVSEEFGLYFARIPLKSSPHVVHGNALRIDWNEVLPAERCS
                     YVLGNPPFVGKQHQNPQQKADVAPLFDRLEGGGVLDFVAAWYIKGAHYARLNHALRVG
                     YVSTNSIVQGEQVGVLWSWMRAQGMHTHFAHQTFKWSNEASGKAAVHCVIVGFAAQES
                     AEKWLYEYAQIDGEPLAVQAQHINPYLVDAADAFLVSRRQSICPVPPVVFGSMPNDGG
                     HLFLDDEEKAALLQECPTAAAWVRPFLGADEFINNIPRWCLWLQDCPPAQLRAMPAVM
                     QRVQKVKALRAASSRAATQALAATPALFGEIRQPKGSYLLIPRHSSERREIIPMGFLE
                     PEVIVGDANLCMPDATLLHFGVMTSRMHMAWVKYVCGRIKSDYRYTSQIVYNNFPWPD
                     LPPHLEPNKPPTPAHKAQIAIETAAQAILDARAKFPGSSLADLYDPLTMPPLLLKAHQ
                     KLDAAVDAAYALCGGKKTWNNDAERVAFLFERYVQLTSLLPAPKAKPARKRPAP"
BASE COUNT          821 a         1331 c         1404 g          791 t
ORIGIN      
        1 ccctgcgccc gggcgacggc gtgatccaca gctggctcaa ccgcctgctg ctgcccgaca
       61 ccgtgggcac cggcggcgac tcgcacacgc gcttccccat cggcatcagc ttcccggcag
      121 gctcgggcct ggtggccttt ggcgccgcca ccggcgtcat gccgctcgat atgcccgaat
      181 cggtgctggt gcgcttcaag ggcaagctgc aaccgggcgt gaccctgcgc gacctggtgc
      241 acgcgattcc gctgtacgcc atcaaggcag gcctcttgac cgtggccaag gccggcaaga
      301 tcaacgtctt ctcgggcaag atcctggaaa tcgaaggcct gccagacctg aaggtcgagc
      361 aagcgtttga gctgtccgac gcgtcggccg agcgcagcgc cgctggctgc accatcaagc
      421 tcaaccccga gccgatcaag gagtacctga ccagcaacat cgttctcatg aagaacatga
      481 ttgccgacgg ctacgcagac gccaagaccc tggcgcgccg catcgagaag gtcgaagcct
      541 ggctggccaa gcctgacctg ctcgaagccg acaaggacgc cgagtacgct gccgtgatcg
      601 agatcgacat gaacgacatc aaggagccca tcgtctgctg ccccaacgac ccggacgacg
      661 ccaagttcct gtctgaagtg gccggcacca agatcgacga agccttcatc ggttcgtgca
      721 tgaccaacat cggccacttc cgcgcagccg ccaagctgct gggcggccag cgcgacatcc
      781 cgaccaagct gtgggtggcc ccgcccacca agatggacga gagcgagctg atcaaggaag
      841 gccactacgc ggcgtttggc acggccggcg cgcgcaccga aatgccgggc tgcagcctgt
      901 gcatgggcaa ccaggcgcag gtgcgcgaag gtgcaaccgt catctcgacc agcacgcgga
      961 acttccccaa ccgcctgggc aagaacacca acgtgttcct gggctcggcc gaactcaccg
     1021 ccatcgcctc gcgcttgggg cgtctgccca cccccgccga gtacctggcc gagatcggcg
     1081 tggtcgatgc caacaaggac agcatctacc gctacatgaa cttcgaccag atcgaggaat
     1141 acgccgaagt ggccaagggc gtgacggcct gatggtttga ggccagcgca cgctggccct
     1201 gaagcacaaa acccccgccg gcgtgaaccg ggcgggggtt ttgtttttgg ggccggacag
     1261 aactagccgg tcaaaacgct gcctcagtgc tgcgcaaagt cagcaatgcg ctgctcttca
     1321 tggtcaggaa cggtttgcac cgccaagcta tgcagatcgc tgcaaatgtc caggtaggcg
     1381 gcgtggcgtt gtgtgtggtc aggtgcagga ggtttgatgg cgaacagcac cgattcgggc
     1441 aggagattgc gccccctgag ccgtttgacc ttttgcagcc aagcgtcgcc gtgctcgtaa
     1501 atatccaccg gcgcctcctg gttcagacgc aagggcttga tgatcttggt caacacatcg
     1561 ccctgctgct gcaccaaggg gaagctggca tggaacacct cgtcgccgac acgctggcgt
     1621 gcaaaaggtg cggctagcgg caggctgctc agcaagtact tgatgcgttt ttccatcgtc
     1681 tgctcgacgt attcaggcgt gatgaacctg cgctccacgc agtgctcgaa ctgccgctgc
     1741 aactccgttg cagggtctgg cgtcagcacg gcgcgcgccg ggccaaagcg caccatggcc
     1801 tcgcgggggt ggatcaatgc gtcaaacaaa tggcgcagcg catcgctgcg gccttgggcg
     1861 ggggtgtcgg caacccgctg ggccacgcgc tgcaattcgt cctgcatgga cttgatggcg
     1921 cgcagataga tctcgcgcgg caactcatca aaaaaatcgg tgatgcgttt ggcccgctgg
     1981 gtttgcagct tgaagccgaa gtagccggtt tcggggcaca gcaagatgac accggcgttg
     2041 gcaaattccc ccgtttcgcg gaaaggcgaa aactgaagca cggcgtaccg acatgcgacg
     2101 ggtttcatgg cgctctccag aaatcagggg tggcacatcg ctccagctgg cgcagcgctt
     2161 ggacgctgtc gaatttggct ggaacatcga actcggaatt ttcctacaac cactcctctg
     2221 gggccgtcgc aaaggctgtt gccgctgcgc ccatggcgcc atgcagccat tgggcgtgct
     2281 gggcctgttc aaccaagtcg tgggtgagcg caggccattg atcggcaaac acatggtgat
     2341 caataaattc cgcagcgtcg aagttgttgc cgaaggcgag gttatggtct atcacgacca
     2401 atgattgatg tgcggtgtcc caaagcaagt tggtgttgcc gcgcagacgg tcggtattgc
     2461 gcacccacca gtcaaacgcc agtaccttgc gctgcagctc tggggcgacc ttgtggtcga
     2521 tgcctaattc gagccacacc gtgtgcacca cctcgcgcga gccgaaggcg ggcaggctgc
     2581 ccacggcctg caagtccttg ggcagctctc gcaccagggc ggggtcgagc tgcaccaggg
     2641 agaagggcgg caagggcagg cccagcgcct gcgccagatg ggcgcaaatc cactcactcc
     2701 acaggctcgc gcggttggtc tgctggccct tgacgtaata gcgctggtcg tcggcgccaa
     2761 cgcacagcaa gggccgcgat agcccctgct gggccgggcc gatcacctcc acgatgggga
     2821 tcatggcggt gctcatggtg ctgggcgttt tctggccggc ttggccttgg gcgcgggcag
     2881 caagctggtc agctgcacat agcgctcaaa caaaaaggcc acgcgctcgg cgtcgttatt
     2941 ccaggttttc ttgccgccgc acagggcgta ggcggcatcg accgcggcgt cgagcttttg
     3001 gtgcgccttg agcagcaggg gcggcatggt cagcgggtcg tacaggtcgg ccaggctgct
     3061 gccggggaac ttggcgcggg cgtcgaggat ggcttgtgct gcggtttcga tagctatttg
     3121 cgctttatgg gcgggcgttg gaggcttgtt tggctctaaa tggggcggca gatcgggcca
     3181 ggggaagttg ttgtagacga tctggctggt gtagcggtaa tcgcttttga tgcggccgca
     3241 aacgtacttg acccaggcca tgtgcatgcg cgaggtcatg acgccgaagt gcaacagggt
     3301 ggcgtcgggc atgcagaggt tggcatcgcc cacgatgacc tcgggctcca aaaaacccat
     3361 gggaatgatt tccctgcgtt ctgacgaatg acggggtatc aacaaatagg atccctttgg
     3421 ctggcgaatt tccccaaata gtgcaggggt ggccgccagc gcttgggtgg ctgcgcggct
     3481 gctggcagcg cgcagcgcct tgaccttctg cacccgctgc atcaccgcag gcatggcgcg
     3541 cagttgggcg ggcggacagt cctgcagcca caggcaccag cgagggatgt tgttgatgaa
     3601 ttcgtcggcg cccagaaagg gccgaaccca cgcggcggcg gtggggcact cttgcagcag
     3661 ggcggctttt tcttcgtcgt ccagaaacag gtggccgcca tcgttgggca tggagccaaa
     3721 cactacgggc ggcacagggc aaatgctttg cctgcgcgag accagaaagg cgtcggcggc
     3781 atcaaccaga taagggttga tgtgctgggc ctggacggcc aggggctcgc cgtcgatctg
     3841 ggcgtattcg tagagccatt tctcggcgct ctcctgcgcc gcaaagccaa cgatcacgca
     3901 atgcacggcg gctttgccgc tggcctcgtt gctccatttg aaggtttggt gggcaaagtg
     3961 ggtgtgcatg ccctgggcgc gcatccaact ccagagcacc ccgacctgct cgccttgcac
     4021 gatgctattg gtggacacat agcccacccg cagggcatgg ttgagccgcg catagtgggc
     4081 ccctttgatg taccaggccg ccacaaaatc gagcacgccg ccgccttcca ggcggtcgaa
     4141 gagcggtgcc acatcagcct tttgctgcgg attttggtgc tgcttgccta cgaacggcgg
     4201 attgcccagc acatagctgc agcgctcggc cggcagcacc tcgttccagt cgatgcgcag
     4261 cgcgttgcca tgcaccacat gcgggctgct tttgagcggg atgcgcgcga agtacaggcc
     4321 aaactcttcg ctcacgcgca ggttcat
//
LOCUS       DAOMTQ010000207         4326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5381_length_4326_cov_24.459611, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000207 DAOMTQ010000000
VERSION     DAOMTQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5381_length_4326_cov_24.459611"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..842
                     /locus_tag="PK518_14455"
                     /pseudo
     CDS             4..842
                     /locus_tag="PK518_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000182.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(997..1797)
                     /gene="fabI"
                     /locus_tag="PK518_14460"
     CDS             complement(997..1797)
                     /gene="fabI"
                     /locus_tag="PK518_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRM50160.1"
                     /translation="MGFLAGKKLLITGVLSNRSIAYGIARACHQQGAELAFSYVGERF
                     KDRISEFAAEFGSKLVFDCDVADDAQIEKMFADLSATWGKFDGFVHSIGFAPREAIAG
                     NFLEGLSREGFRIAHDISAYSFPAMAKAALPYLNDKSSLLTLSYLGALRSIPNYNTMG
                     LAKASLEASVRYLAEAVGRTADGRAIRANGISAGPIKTLAASGIKDFGKLLGRVADAA
                     PLRRNVTIEDVGNVAAFLLSDLASGVTAEITYVDGGFSQTAGLSADQV"
     gene            1844..3667
                     /locus_tag="PK518_14465"
     CDS             1844..3667
                     /locus_tag="PK518_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM50161.1"
                     /translation="MRYWLICSLFFPCLALWPGAPARAAHAYAMWGDPALPAGFAHLP
                     YVNPDAPKGGELRLVSNLRASTFDKYNPFTIRGNAPAYLSALMFDTLLSGSMDETATG
                     YGLLAEDVSVAPDGLSATFRLRPAARFHNGKPVTAQDVKHSYDTLVGPYTSPGFKTLL
                     IEVAGVDVLDDHTVRYRFKAPNRELPLTVGGLPVFSRDWGLGEDGKPKPFDQVVMDIP
                     IGSGPYRVGPVNFGKDITYVRDPNYWARDLNVRRGTANFDRVLIKIYKDNTARLEALK
                     AGEFDLMRFFSAGDWARRVNGKKFDTGELVKAEFANKLPTGFQSYVLNTRRPLLADRR
                     VREALGLAFDFEWMSRQLFYGAYRRVHGIFGNTMCETHGLPTAAELALLEPWRKNLPP
                     EVFGPMFEPPVTKDAMALREHLRRALVLLKEAGWEVKDGVLRNAQGEPFVLEYMDSNE
                     GGVRTLSSWQRNLAKLGITLRFRSVDFALYQQRLQKFDFDITTIAYQGTNNPGQEFAD
                     LFGSKAADQEDSGNFPGVKNPAVDAMIRAMVSAKTLQQMLPACHDLDRIIAHEHYLIP
                     QWSASTHRMVYDAWRLAKPPVVPPYSPGEMWVIDTWWSKKP"
     gene            3713..4180
                     /locus_tag="PK518_14470"
     CDS             3713..4180
                     /locus_tag="PK518_14470"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRM50162.1"
                     /translation="MFNLEHKIPPPVVGLACAGLAWLLAGAAPALALPWSARLALAVA
                     LLLALAGLALELWGLWVFRCHRTTPNPLAPERARAVVQSGPYRFTRNPMYVGVALQLL
                     GWCAYLRNPLALLALVAFVAYITRFQILPEERALAQNFGEPYRQYLRLVRRWL"
     gene            4196..>4326
                     /locus_tag="PK518_14475"
     CDS             4196..>4326
                     /locus_tag="PK518_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837909.1"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HRM50163.1"
                     /translation="MFAYILKRILLMLPTLLGVLLLTFVVIQFVPGGPVEQYLAEAKA
                     "
BASE COUNT          737 a         1384 c         1429 g          776 t
ORIGIN      
        1 cacatatatg aaagatcgac tcccaagccc agaaggagaa ctgaaaggac agatgtaacc
       61 gtagtcgtcg gcgccggcgg catcggcttg gcgattgtcc accgcatcag cagcggccgc
      121 catgtcctgc tctccgatca ccgtcaggaa gccgctgacg cggccgccaa attgctcgag
      181 aacgcgggat tcgaaaccac ggccatgcag gccgatgttt cggatcgaaa ctcgatccag
      241 gccgtgatcc gcaaggcgca ggaactgggg cagatcaagg cgctggtgca ggtggcgggc
      301 gtgtcgccct cgcaggcgtt catcgaggcg atcctcaagg ttgatctgta cggcatctcg
      361 atgctgctgg aagagttcgg ccgggtgatt gccaaaggcg gcttgggcgt ggtgatctcc
      421 tcgcagtccg gctaccgcat gccggcgctg actgccgcac aggatgcgct gctggcgacc
      481 gcgccggcgg acgacttgct ggcactcgac ttcgtcaaga acgtcgaaag cacgctgcat
      541 gcgtgcaact cgctgcgcgt gcgcggcgag gcagtgcagt gggccaagcg cggcgcgcag
      601 gtcaattcga tccgcccggc atcatcgtga agccgctggc gcacgacgaa ctgcatggcg
      661 aacgcgcgga cttctaccag gcgatgctga agaagatgcc agccggacgc gccggtacgc
      721 cggacgaagt ggctgcgctg atcatggggc cggaaggcgg ctttatcact ggcagtgaat
      781 tcttgatcga cggtggtgcg acggccaatt tctactacga tccggacgct ggcaaagcgt
      841 gacggaccag aagaagcgca atctgcagac cggtttctga tgggtgacgg aaagtcatca
      901 agaccaccag tccgcacaag tatgcgcagt attggcgcta aaaagccctg cgcaccgcat
      961 tggccgcagg gctgggtttt ttggctttgc ggcggtttat acctgatccg cgctcagccc
     1021 cgccgtctgg ctgaagcccc cgtccacgta ggtgatctcg gccgtcacac cgctggccag
     1081 gtcggagagc aggaaggccg ccacattgcc cacgtcctcg atcgtcacgt tgcggcgcag
     1141 cggggcggcg tcggccacgc ggccgagcag tttgccgaag tccttgatgc cgctggcggc
     1201 cagggttttg atggggccgg cgctgatgcc gttggcgcgg atggcgcggc cgtccgccgt
     1261 gcggcccacg gcctctgcca gatagcgcac gctggcctca aggctcgcct tggccaggcc
     1321 catggtgttg tagttgggga tgctgcgcag cgcgcccagg taggacaggg tcaagagcga
     1381 cgatttatcg ttcaggtagg gcagggcggc cttggccatg gccgggaagc tgtaggcgct
     1441 gatgtcgtgc gcgatgcgaa agccctcgcg tgacaggcct tcgaggaagt tgccggcaat
     1501 cgcctcgcgc ggcgcaaagc ctatgctgtg cacgaagccg tcaaacttgc cccaggtggc
     1561 ggacaggtcg gcaaacatct tctcgatctg tgcatcgtcg gccacgtcgc agtcaaacac
     1621 cagcttggag ccgaactcgg ccgcaaactc gctgatgcga tccttgaagc gctcgcccac
     1681 gtagctgaag gcgagctcgg ccccctgttg gtggcaggcg cgggcgatgc cgtaggcgat
     1741 ggagcggttg gacagtacgc ccgtgatgag cagcttcttg ccggcgagaa aacccatatt
     1801 cgttctcctg tggatgcgtt cagaggtaat gcagaattgt cgcatgcggt attggctgat
     1861 ctgttcactg ttcttcccgt gcctggcgtt gtggcccggc gcgcctgcgc gggccgcgca
     1921 cgcctatgcc atgtggggcg atcccgcctt gccggccggt tttgcgcacc tgccctatgt
     1981 gaaccccgat gcgcccaagg gcggcgagct gcgcctggtg agcaacctgc gcgcatccac
     2041 cttcgacaag tacaacccgt tcaccatccg cggcaacgcg ccggcctacc tgtcggcgct
     2101 gatgttcgac acgctgctgt ccggctcgat ggacgagacc gccaccggct acggcctgct
     2161 ggccgaggac gtcagcgtgg cgcctgacgg tctgtcggcc accttccgcc tgcggcccgc
     2221 agcgcgcttt cacaacggca agccggtcac ggcccaggat gtgaagcaca gctacgacac
     2281 gctggtcggc ccctacacct cgccaggctt caagaccctg ctgatcgagg tggccggcgt
     2341 ggatgtgctg gacgatcaca cggtgcgcta ccgcttcaag gcgcccaacc gcgagctgcc
     2401 gctgaccgtg ggcggcctgc cggttttcag tcgcgactgg ggcctgggcg aggacggcaa
     2461 gcccaagccc tttgaccagg tggtcatgga tattccgata ggcagcgggc cgtatcgtgt
     2521 tggcccggtc aacttcggca aggacatcac ctacgtgcgc gaccccaact attgggcgcg
     2581 cgacctcaac gtgcgccgcg gcacggccaa cttcgaccgg gtgctgatca agatctacaa
     2641 ggacaacacg gcacggctgg aggcgctgaa ggccggcgag ttcgacctga tgcgtttttt
     2701 cagcgccggc gactgggcgc ggcgcgtgaa cggcaagaag tttgataccg gcgagctggt
     2761 caaggccgaa tttgccaaca agctgcccac gggcttccag agctatgtgc tgaacacgcg
     2821 ccggccactg ctggcagacc ggcgcgtgcg cgaggccctg ggcctggcgt tcgactttga
     2881 atggatgagc cgccagttat tctacggcgc ctaccggcgc gtacacggca tcttcggcaa
     2941 caccatgtgc gaaacccatg gcctgcccac tgcggcggag ctggccctgc tggagccctg
     3001 gcgcaagaat ttgccgcccg aggtcttcgg ccccatgttc gagccgccgg tcaccaagga
     3061 cgccatggcg ctgcgcgagc acctgcggcg cgccctggtg ctgttgaaag aagccggctg
     3121 ggaggtcaag gacggcgtgc tgcgcaacgc ccagggcgag ccttttgtgc tggagtacat
     3181 ggacagcaac gagggcgggg tgcgcacgct gtcgtcgtgg cagcgcaacc tggcgaagct
     3241 gggcataacg ctcaggttcc gctcggtgga ctttgcgctc taccagcagc ggctgcagaa
     3301 gttcgacttc gacatcacca ccatcgccta ccagggcacg aacaaccccg gccaggagtt
     3361 cgccgacctg ttcggcagca aggcggcgga ccaggaggac tcgggcaact tccccggcgt
     3421 gaagaacccg gccgtggacg cgatgatccg cgccatggtg tcggccaaga cgctgcagca
     3481 gatgctgccc gcctgccacg acctggaccg catcatcgcc cacgagcatt acctgatccc
     3541 gcaatggtct gcctccacgc accgcatggt gtatgacgcc tggcgcctgg ccaagccccc
     3601 ggtggtgccg ccgtactcgc cgggcgagat gtgggtcatc gatacctggt ggtctaaaaa
     3661 accatagctg tttgcgcttg atggataagg gttacagcct gttttaatca aaatgttcaa
     3721 tctggaacac aagatccccc cgccggtcgt cggcctggcc tgcgccggcc tggcctggct
     3781 gctggccggc gccgcgccag ctttggcgtt gccgtggtcg gctcggctgg cgctggccgt
     3841 ggcgctgctg ctggccctgg ccggcctggc gctggagctg tggggcctgt gggtgtttcg
     3901 ttgccaccgc accacgccca acccgctggc gcccgaacgc gcgcgtgccg tggtgcagtc
     3961 cgggccgtac cgcttcacgc gcaatcccat gtatgtcggc gtggccctgc agctgctggg
     4021 ctggtgcgcg tacctgcgca acccactggc gctgctggcg ctggtggcat tcgtggccta
     4081 catcacgcgc tttcagatcc tgcccgagga gcgggcgctg gcacagaact ttggcgagcc
     4141 ataccgccag tacctgcgcc tggtgcggcg ctggctctga atatcatcga cgcccatgtt
     4201 tgcctacatc ctcaagcgca tcctgctcat gctgcccacg ctgctgggtg tgctgctgtt
     4261 gaccttcgtc gtcatccagt tcgtgcccgg cggcccggtg gagcagtacc tggccgaggc
     4321 caaggc
//
LOCUS       DAOMTQ010000208         4263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5501_length_4263_cov_24.672529, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000208 DAOMTQ010000000
VERSION     DAOMTQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5501_length_4263_cov_24.672529"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1386)
                     /gene="glcF"
                     /locus_tag="PK518_14480"
     CDS             complement(148..1386)
                     /gene="glcF"
                     /locus_tag="PK518_14480"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HRM50164.1"
                     /translation="MQTQLAPEYQGTPEGQEAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDSPRGRIYLIKQVLEGMEPTRKTQAHLDRCLTCRNCETTCPSGVQYGNLVDIGRRIV
                     EEKVQRPLGERAVRWALKEVLPSPLFGPAMKAGRAVRGLLPEGLRDKVQPHPPAGAWP
                     RRSHARKVLLLEGCVQPSMAPNINSATARVLDAAGIETLTAPEAGCCGAVKFHLNDQD
                     GGRAQMRANIDAWWPYIERGEVECLVMNASGCGVTVKEYGHVLARDAQYADKAARISS
                     LTRDLSELLPGLVPLLKDRVRAAGRAFAYHPPCTLQHGQKLRGGVERHMAALGFTLRV
                     ARTESHLCCGAAGTYSVLNPELSRQLRERKLGALGEAFADAPPAAILSANMGCITHLQ
                     GGTDIPVRHWVELLDEALLA"
     gene            complement(1418..2527)
                     /gene="glcE"
                     /locus_tag="PK518_14485"
     CDS             complement(1418..2527)
                     /gene="glcE"
                     /locus_tag="PK518_14485"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HRM50165.1"
                     /translation="MDAALSQIIDRVRDSAARQAPLCIRGGGTKDFHGPGAPRHAGEP
                     LDMRPLAGIVSYEPSELVVTALAGTPLAELEALLARHGQCLAFEPPRFAAGGTVGGMV
                     AAGLSGPARASVGAVRDFVLGVEIINGRGELLRFGGQVMKNVAGYDVSRLMAGSWGQL
                     GVITEVSLKVLPVAPAEATLRFDCGQAEALKMLHAWGGQPLPLNASCWLEEGGAGQLY
                     VRLRGARAAVEAACRDMGGEPQDAPNVADDWQACRDQRLPWFAARPADQALWRLSLPQ
                     TAPVLPLPAGVAAPLIEWQGGLRWLQAPMHHREALRTLALQVGGSASCFIAAGADGMG
                     DKGIFDTNSGALATIHRRLKQAFDPAAILNPGRLL"
     gene            complement(2628..3932)
                     /gene="amt"
                     /locus_tag="PK518_14490"
     CDS             complement(2628..3932)
                     /gene="amt"
                     /locus_tag="PK518_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRM50166.1"
                     /translation="MRKVHAFLVSGLCLLAGAACAQAPAAPRIDSGDTAWMLTSTLLV
                     ILMTIPGLALFYGGLGRAKNMLSVLVQVFVIFALCSLLWAIYGYSLAFSGEGKFVGDL
                     SKMFLKGVGLESFGALTTIPEYVFFAFQGTFAAITVALIVGSFAERMRFSAVLIFAVL
                     WFSFSYVPMAHIVWGGGLLAADGALDFAGGTVVHINAGIAGLVGAYMVGKRIGFGREA
                     FTPHSLTLTMVGASLLWVGWFGFNAGSAGAANAVAGLAFVNTVLATAAATLSWSAGEA
                     VHKGKASMLGAASGAVAGLVAVTPAAGFVGPMGAIAIGLVSGLVCLWGVGGLKRMLKV
                     DDAFDVFGVHGLGGIVGAILTGVFAAQGLGGTGGLTPDTFSMGAQLWIQVKSVLFTIV
                     WSGVVSFVAYKIADLLVGLRVSEEAERQGLDISYHGETAYNR"
     gene            complement(3955..>4263)
                     /locus_tag="PK518_14495"
     CDS             complement(3955..>4263)
                     /locus_tag="PK518_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRM50167.1"
                     /translation="FKLDEVREALSDIGVQGITVTEVKGFGRQKGHTELYRGAEYVVD
                     FLPKVKVEAAVDDALVERAIEAIEGAARTGKIGDGKIFVAALEQVVRIRTGETGVQAL
                     "
BASE COUNT          663 a         1531 c         1468 g          601 t
ORIGIN      
        1 gctgggcgtg cggcgcggct gcggcctctg cggcggcggc gggtggcgtg gcctcggcgg
       61 ggggcgtcgg caggtcgttc acgggatcgt tcatggctca gaaacgcgcg gcatggcgca
      121 tgcggcgcgg cgggtcaaaa cccgccatca tgccagcaat gcctcgtcca gcagctccac
      181 ccagtggcgc acggggatgt cggtgccgcc ttgcaggtgg gtgatgcaac ccatgttggc
      241 cgacaggatg gccgcgggcg gcgcgtcggc aaaggcctcg cccagggcgc ccagcttgcg
      301 ctcgcgcagc tgccgcgaca gctcggggtt gagcaccgag taggtgccgg ccgcgccgca
      361 gcacaggtgc gattcggtgc gcgccacgcg cagcgtgaag cccagggccg ccatatgccg
      421 ctccacgccg ccgcgcagct tctggccatg ctgcagcgtg cagggcgggt ggtaggcaaa
      481 ggcccggccg gcggcgcgca cgcggtcttt caagagcggc accaggcccg gcagcagctc
      541 ggacaggtcg cgcgtcaggc tgctgatgcg cgccgccttg tcggcgtatt gcgcgtcgcg
      601 cgccaggaca tggccgtact ccttgaccgt gacgccgcag cccgaggcgt tcatgaccag
      661 gcattcgacc tcgccgcgct cgatgtaggg ccaccaggcg tcgatgttgg cgcgcatttg
      721 cgcgcggccg ccgtcctggt cgttcaggtg gaacttgacg gcgccgcagc agccggcctc
      781 gggggcggtc agtgtctcta tgcccgcggc gtcgagcacg cgcgccgtgg cgctgttgat
      841 gttgggcgcc atgctgggct gcacgcagcc ctccagcagc agcaccttgc gcgcatggct
      901 gcgccggggc caggcgccgg ccggcgggtg gggctgaacc ttgtcgcgca agccctcggg
      961 cagcaggccg cgcacggccc ggccggcctt catggcaggc ccgaacagcg gcgagggcag
     1021 cacctccttg agcgcccagc gcacggcgcg ctcgcccagg gggcgctgca ccttttcctc
     1081 gacgatgcgc cggcctatgt ccaccaggtt gccgtattgc acgccgctgg ggcaggtggt
     1141 ttcgcagtta cggcaggtca ggcagcggtc cagatgcgct tgggtcttgc gcgtgggctc
     1201 catcccttcg agcacctgct tgatcaggta gatgcgcccg cgcgggctgt ccagctcgtc
     1261 gcccagcagc tggtaggtgg ggcaggtggc ggtgcaaaag ccgcagtgca cgcatttgcg
     1321 caggatggcc tcggcctcct gcccttcggg cgtgccctgg tactcgggtg cgagctgggt
     1381 ttgcatgggt gctttcctgt atgggcctgc cgcgcggcta caggagccgg ccggggttga
     1441 ggatggcggc cggatcgaac gcctgcttca ggcgccggtg tatcgtcgcc aacgccccag
     1501 aattggtatc aaaaatgcct ttatcgccca tcccatcagc gccagcagct ataaaacaag
     1561 aagcactacc accgacctgc agggccagcg tgcgtaacgc ctcgcggtgg tgcatgggcg
     1621 cctgcagcca gcgcaggcca ccctgccatt cgatgagcgg cgccgccacg cccgcgggca
     1681 gtggcagcac cggcgccgtc tgcggcaggg acaggcgcca cagcgcctgg tcagccgggc
     1741 gcgccgcaaa ccagggcaga cgctggtcgc ggcaggcctg ccagtcgtcg gccacattcg
     1801 gcgcgtcctg cggctcgccg cccatgtcgc gacaggcggc ctccaccgcc gcccgtgcgc
     1861 cgcgcaggcg cacataaagc tggcccgcgc cgccctcttc cagccagcag ctggcattca
     1921 aaggcagggg ctggccgccc caggcgtgca gcatcttcag cgcctcggcc tggccgcagt
     1981 caaagcgcag cgtggcctcg gccggcgcca cgggcagcac cttcaggctg acctcggtga
     2041 tcacgcccag ctggccccag ctgcccgcca tcaggcgcga gacgtcgtag ccggcgacgt
     2101 tcttcatcac ctggccgcca aagcgcagca gctcgccacg gccgttgatg atctccacgc
     2161 ccagcacgaa gtcgcgcacc gcgcccacgc tggcgcgcgc cgggcccgac aggccggcgg
     2221 ccaccatgcc gccgacggtg ccaccagcgg caaagcgcgg cggctcgaag gccaggcatt
     2281 ggccatgccg ggccagcagc gcctccagct cggccagcgg cgtgccggcc agggccgtca
     2341 ccaccagctc gctgggctcg tagctgacga tgccggccag gggccgcatg tccagcggct
     2401 cgcccgcatg gcgcggcgcg ccggggccgt gaaagtcctt ggtaccgccg ccgcggatgc
     2461 acagcggggc ctggcgcgcg gcgctgtcgc gcacgcggtc gatgatctgg ctgagggctg
     2521 cgtccatggc ggccatggta ctgccggccc cgcgcgcggc gggcgggcgc gggtttgatt
     2581 tttttgaacg cgcggcgtga aggctttgtg cccagccgca cccaccatca acggttgtac
     2641 gccgtctcgc cgtggtagct gatgtccagg ccctggcgct cggcctcctc gctcacgcgc
     2701 aggcccacca gcaggtcggc gatcttgtag gccacgaagg acaccacgcc ggaccaaacg
     2761 atggtgaaca gcacgctctt gacctgtatc cacagctgcg cgcccatgct gaaggtgtcg
     2821 ggagtcaggc ccccggtgcc acccaggccc tgggccgcga acacgccggt gaggatggcg
     2881 ccgacgatgc cgcccaggcc atgcacgccg aacacgtcga aggcgtcgtc caccttgagc
     2941 atgcgcttca agccgcccac gccccacagg cagaccaggc cggagaccag gccgatggcg
     3001 atggcgccca tgggcccgac gaagcccgcg gccggcgtga ccgccaccag gccggcgacg
     3061 gcgcccgagg ccgcgcccag catcgatgcc ttgcccttgt gcacggcctc gcccgcgctc
     3121 cacgacagcg tggcggcggc ggtggcgagc acggtgttga cgaaggccag gccggcgacg
     3181 gcgttggcgg cgccggccga gccggcgttg aagccgaacc agcccaccca cagcagcgac
     3241 gcgcccacca tggtcagcgt caacgagtgc ggcgtgaacg cctcgcggcc aaagcctatg
     3301 cgcttgccca ccatgtaggc gcccaccagg ccggcgatgc cggcgttgat gtgcaccacg
     3361 gtgccgccgg caaagtccag cgcgccatcg gccgccagca gaccaccacc ccagacgata
     3421 tgcgccatgg gcacgtagct gaagctgaac cacagcacgg cgaagatcag cacggccgag
     3481 aagcgcatgc gctcggcgaa cgagcccacg atcagcgcca cggtgatggc cgcgaacgtg
     3541 ccctggaagg cgaagaacac gtattcgggg atggtggtca gcgcgccaaa actctccagg
     3601 cccacgccct tgaggaacat cttggacagg tcgcccacga acttgccctc gcccgagaac
     3661 gccaggctgt agccgtagat ggcccacagc agcgagcaca gggcaaagat cacgaacacc
     3721 tgcaccagca ccgacagcat gttcttggcc cgccccaggc cgccgtagaa cagcgccagg
     3781 ccggggatgg tcatcaggat caccagcagc gtcgaggtca gcatccaggc cgtgtcaccc
     3841 gaatcgatgc gcggtgcggc cggcgcctgg gcgcatgcgg cgccggccaa taggcacagg
     3901 cctgacacca ggaaagcgtg tacttttctc atgatgggtc tccgtcgtct acggttacag
     3961 ggcctgcacg ccggtctcgc cggtgcgtat gcgcaccacc tgctccagcg cggccacgaa
     4021 gatcttgccg tcgccgatct tgccggtgcg cgccgcgccc tctatggcct cgatggcgcg
     4081 ctccaccagc gcatcgtcca ccgcggcctc gaccttcacc ttgggcagaa agtccaccac
     4141 atactcggcg ccgcggtaca gctcggtatg gcccttctgg cggccaaagc ccttgacctc
     4201 ggtgacggtg atgccctgca cgccgatgtc cgacagcgcc tcgcgcacct cgtcgagctt
     4261 gaa
//
LOCUS       DAOMTQ010000209         4182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5666_length_4182_cov_27.614248, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000209 DAOMTQ010000000
VERSION     DAOMTQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5666_length_4182_cov_27.614248"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PK518_14500"
     CDS             <1..693
                     /locus_tag="PK518_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM50168.1"
                     /translation="SLMVPRADVVCLDVLESFEENLRRIDESEHARFPVVRGGMENIL
                     GVLSARQWLSRAVRDPAARELGALALHTALYVPETINAKELLDNFRQSGMHMAFVIDE
                     YGEVQGIVTVQDLIEAITGEFQSLDPDDSWAVQRDDGSWLLDGHIPVPELKDRLQLAT
                     VPQEDRGRYQTLSGMMMLLTGKLPGVTDTVQWEGWRFEVVDMDGKTIDKVLASRLPEV
                     QLHMGDQAVSGD"
     gene            complement(700..1368)
                     /locus_tag="PK518_14505"
     CDS             complement(700..1368)
                     /locus_tag="PK518_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM50169.1"
                     /translation="MFFVFGPSGQIYRGGSGKLAQLAPVQRVQRPQGLRARALEPDAA
                     PRHTGAVKAQTAQQQRLQEAAAAYVQTEQGPDQPRLPVLRVADMMTVGAVTLPPEMPA
                     HDAWLALARQRVAQAPVVNAQGRVVGLLLRADIAPVDLLPQPAAVPQAIARARRSVSE
                     AMVSPVPTVAAHTELRRVAPVLLDTGLPGLPVTDDAGVLQGFITRTDILRAVVADPPL
                     DLWS"
     gene            1468..2409
                     /locus_tag="PK518_14510"
     CDS             1468..2409
                     /locus_tag="PK518_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRM50170.1"
                     /translation="MIHRAAITAARRQLASQPGFLRTTPLLRLSGRSVGVDCDEVWLK
                     LEQLQVGGSFKARGMLYRLLANPVPDSGVIIASGGNAGIAVAAAAKALGVRCEVFVPE
                     VSPEAKRARLRALGAEVVVTGAAYAEAFQACVARQQATGALQAHAYDQPEVVAGAGTL
                     ALEIEEQGGRLPDSLLVSVGGGGLIGGVAAWVDSRAHVVALEPERAPTLHAARAAGMP
                     VDVEVGGLAADSLGARRIGAIGWEVSQRHVHASLLLPDDAIRAAQLWLWKEFKLAVEP
                     AAALGLAALQTGAYRPQPRETVALILCGANCDPASLA"
     gene            2441..3757
                     /gene="serS"
                     /locus_tag="PK518_14515"
     CDS             2441..3757
                     /gene="serS"
                     /locus_tag="PK518_14515"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRM50171.1"
                     /translation="MLDILLLRKDLASAVARLESRKKPQAFLNVEAFQALEAERKTIQ
                     TRTEELQSQRNQLSKQIGALMGKGEKDAAEAAKAQVAALKNELDGSVVRLEQIQAELQ
                     AMLAAVPNLPHESVPVGADEAGNVELRRWGAPKAYDFAVKDHVDLGAPLGLDFDLGAK
                     LSGSRFTVMQGQMARLHRALAQFMLDVQTGEHGYTECYVPYIVNADSLKGTGQLPKFE
                     GDLFAAKKGGQDGEPVPDNAALYLIPTSEVPLTNIVRDEVLQEAELPIRLTAHSPCFR
                     SEAGSYGRDTRGMIRQHQFDKVEMVQIVHPEKSYQALEEMTGHAEAILQKLGLPYRVV
                     ALCTGDMGFGAAKTYDLEVWLPAQNTFREISSVSNCEAFQARRMQARFKNAQGKNELV
                     HTLNGSGLAVGRTLVAVLENYQNQDGSITVPEALRPYMGGMEILKP"
     gene            3791..3880
                     /locus_tag="PK518_14520"
     tRNA            3791..3880
                     /locus_tag="PK518_14520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3825..3827,aa:Ser,seq:cga)
     gene            complement(4049..4174)
                     /locus_tag="PK518_14525"
     CDS             complement(4049..4174)
                     /locus_tag="PK518_14525"
                     /inference="COORDINATES: protein motif:HMM:NF017343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HRM50172.1"
                     /translation="MRYMPGVTAQDRVIHNGTIYGIESLIDVRSDHRELVLMCKG"
BASE COUNT          710 a         1408 c         1415 g          649 t
ORIGIN      
        1 tcgctgatgg tgccgcgcgc cgacgtggtg tgcctggacg tgctggagtc gttcgaggaa
       61 aacctgcgcc gcatcgacga gtccgaacat gcgcgctttc cggtggtgcg cggcggcatg
      121 gagaacatcc tgggcgtgct cagcgcgcgc caatggctgt cgcgcgcggt gcgcgacccg
      181 gccgcgcgcg agctgggcgc gctggcgctg cacacggcgc tgtacgtgcc cgagaccatc
      241 aacgccaagg agctgctgga caacttccgc cagtccggca tgcacatggc cttcgtcatc
      301 gacgagtacg gcgaggtgca gggcatagtc accgtgcagg acttgatcga ggccatcacc
      361 ggcgaattcc agtcgctgga cccggacgac tcctgggccg tgcagcgcga cgatggcagc
      421 tggctgctgg acggccatat tcccgtgcct gagctcaagg atcgcctgca gctggccacc
      481 gtgccgcagg aggatcgcgg gcgctaccag acgctcagcg gcatgatgat gctgctcacc
      541 gggaagctgc ctggggtcac cgacaccgtg cagtgggagg gctggcgctt cgaggtggtg
      601 gacatggacg gcaagaccat agacaaggtg ctggccagcc gcctgcccga ggtgcagttg
      661 cacatgggcg accaggccgt gagcggggac tgatcgccct cagctccaca gatcgagtgg
      721 cggatcggcc accacggcgc gcagtatgtc ggtgcgcgtg atgaagccct gcagcacgcc
      781 ggcatcgtcc gtcacgggca ggcccggcag gcccgtgtcc agcagtaccg gcgccacgcg
      841 gcgcagctcg gtgtgggcgg ccacggtggg caccgggctg accatggcct cggacacgct
      901 gcggcgtgcg cgtgcaatgg cctgcggcac ggcggccggc tggggcagca gatccacggg
      961 agcgatgtcg gcgcgcagca gcaggccgac cacccgcccc tgggcgttga ccaccggcgc
     1021 ctgggccacg cgctgccgtg ccagcgccag ccaggcgtca tgggccggca tctcgggcgg
     1081 cagggtgacc gcacccacgg tcatcatgtc ggccacgcgc agcaccggca ggcgcggctg
     1141 atcggggccc tgctcggtct gcacataggc ggcagcggcc tcctgcaggc gttgctgctg
     1201 cgccgtctgg gccttgacgg cgccggtgtg ccggggcgct gcatcgggct ccagggcgcg
     1261 tgcgcgcagg ccctgcgggc gctgcacgcg ctgcaccggc gccagctggg ccagcttgcc
     1321 cgagccgccg cggtagatct gccccgaagg accaaacaca aaaaacatga ccacctcctg
     1381 catggccccc ggtggacctg ccatcgccat gttatcgaca gccgtgccgg ccactacact
     1441 gcaccctttt tttcgccagg ttcccgcatg atccatcgcg ccgccatcac cgctgcccgc
     1501 cgccagctgg ccagccagcc gggctttctg cgtaccacgc cgctgctgcg cttgtctggc
     1561 cgctcggtgg gggtggattg cgacgaggtc tggctcaagc tggagcaact gcaggtgggt
     1621 ggcagcttca aggcgcgcgg catgctctat cgcctgctgg ccaatcccgt gcccgacagc
     1681 ggcgtcatca tcgcctcggg cggcaacgcc ggcattgccg tggcggccgc cgccaaggcc
     1741 ctgggcgtgc gctgcgaggt gttcgtgccc gaggtctcgc ccgaggccaa gcgcgcccgg
     1801 ctgcgcgccc tgggggccga ggtggtggtg accggcgccg cgtatgccga ggcctttcag
     1861 gcctgcgtgg cccgccagca ggccacgggc gcgctgcagg cccatgccta tgaccagccc
     1921 gaggtggtgg ccggcgccgg cacgctggcc ctggagatcg aggagcaggg cggccggctg
     1981 cccgacagcc tgctcgtcag cgtgggcggc ggcggcctca tcggcggcgt ggccgcctgg
     2041 gtggacagcc gagcccatgt ggtggcactg gagcccgagc gtgcgcccac cctgcatgcc
     2101 gcccgggccg caggcatgcc ggtggacgtg gaggtcggcg gcctggccgc cgattcgctg
     2161 ggtgcgcgcc gcatcggcgc catcggctgg gaggtgagcc agcgccatgt gcacgcatcg
     2221 ctgctgttgc ccgacgacgc cattcgtgcc gcgcaactgt ggctgtggaa ggagttcaag
     2281 ctggccgtgg aacctgccgc agccctcggt ctggctgccc tgcagaccgg cgcctaccgc
     2341 ccgcagccac gtgaaaccgt ggcgctgatc ctgtgcggcg ccaactgcga cccggcaagt
     2401 ttggcctgac cccggcacgc ctgccctcta aaattgcgcc atgctagaca tcctgctcct
     2461 tcgcaaagac ctcgcctcgg ccgtcgcccg gctcgaatcc cgcaaaaaac cccaggcctt
     2521 cctgaacgtg gaggcgttcc aggctctgga ggctgagcgc aagacgatac agacgcgcac
     2581 cgaggagttg cagtcgcagc gcaaccagct gtccaagcag atcggcgcgc tgatgggcaa
     2641 gggtgagaaa gacgccgccg aggccgccaa ggcccaggtg gcagcgctca agaacgagtt
     2701 ggacggctcc gtcgtgcgcc tggagcagat ccaggccgaa ctgcaggcca tgctggcggc
     2761 cgtgcccaac ctgccgcacg agagcgtgcc cgtgggcgct gacgaggccg gcaacgtcga
     2821 gctgcgccgc tggggcgcgc ccaaggccta cgacttcgcg gtgaaagacc atgtggatct
     2881 gggcgccccg ctgggcctgg acttcgatct gggcgccaag ctgtcgggct cgcgtttcac
     2941 cgtcatgcag ggccagatgg cgcgcctgca ccgggcgttg gcgcagttca tgctggacgt
     3001 gcagaccggc gagcatggct acaccgaatg ctatgtgccc tacatcgtca acgccgactc
     3061 gctcaagggc acgggccagc tgcccaagtt cgagggcgac ctgttcgccg ccaagaaggg
     3121 cggccaggac ggcgagcccg tgcccgacaa tgccgcgctg tacctgatcc cgaccagcga
     3181 ggtgccgctc accaacatcg tgcgcgacga ggtgctgcaa gaggccgagc tgcccatcag
     3241 gctcaccgcg cattcgccgt gctttcgctc cgaggcaggc agctacggcc gcgacacgcg
     3301 cggcatgatc cgccagcacc agttcgacaa ggtcgagatg gtgcaaatcg tccaccccga
     3361 gaaaagctac caggcgttgg aagagatgac cggccacgcc gaggccattt tgcaaaagct
     3421 gggcctgccc taccgtgtgg tggctctgtg caccggcgac atgggctttg gcgccgccaa
     3481 gacctacgac ctggaagtct ggctgccggc gcagaacacc ttccgcgaga tcagctcggt
     3541 gagcaactgc gaagccttcc aggcgcgccg catgcaggcg cgtttcaaga acgcacaggg
     3601 caagaacgag ttggtgcaca ccttgaacgg ctcgggactt gccgtgggcc gtacgctcgt
     3661 cgcggtgctg gagaactacc agaaccaaga cggctccatc accgtgcccg aggcgctgcg
     3721 cccctacatg ggcggcatgg aaatcttgaa gccctgattt aactgctaga atcgcaggct
     3781 tcgacacaca ggagaggtgg cagagtggtc gaatgtacct gactcgaaat caggcgtagt
     3841 ggcaacacta ccgtgggttc gaatcccacc ctctccgcca ggctatcaat aaaatcaaca
     3901 acttacagcg gtttttgaaa tctgtaccac ataaaagccc acataaaaca aagccacctt
     3961 gcctagctcg gtggcttttt tcttggctgt gatagctcgc ggtttttcac aaaagcgcca
     4021 ggggggcact gacaaaaccg acgaaaccct accccttgca catcaacacc agctcgcggt
     4081 ggtctgagcg aacatcaatc aagctctcaa tgccgtagat ggtgccgttg tggatcacgc
     4141 gatcctgcgc cgtgacgcct ggcatgtagc gcatcttgat cc
//
LOCUS       DAOMTQ010000210         4152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5739_length_4152_cov_22.481816, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000210 DAOMTQ010000000
VERSION     DAOMTQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5739_length_4152_cov_22.481816"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PK518_14530"
     CDS             <1..479
                     /locus_tag="PK518_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HRM50173.1"
                     /translation="QQRGSDNKALAVGTSAGAAAGAAAGAKVGAMIDVGTGGLTLGLG
                     TALGALLGGGTAWAVRNLQKKDAQLEGAQDLLQHMAEAACTRYLALAHLGRVPAEQAQ
                     ELGARWQAEVTGTVAAHWSELNTALRADPPTSDPLQPLLGSMLRGILQRSFVAEAP"
     gene            complement(562..1854)
                     /locus_tag="PK518_14535"
     CDS             complement(562..1854)
                     /locus_tag="PK518_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HRM50174.1"
                     /translation="MAFSLNATAQSLEAWWVQLSHFPWRTTAHMLRERFREDRLGVTA
                     SSLTFTTLLALVPFFTVALAVFAAFPIFGKMQLMLQRWLIDSLVPDSISRPVLGYLTQ
                     FAAKAGGLGAAGFSILMVTALALILTIDRTLNGIWRVRRLRPLGQRVLIYWAAMTLGP
                     LVLAASLALTTAIAGAASRELGATLPGGARLLLDAIEFLLLAAGVAAMYRYVPNTQVH
                     WRDAWAGSLFVSLAMELAKKGLGLYLAKVPTYSVIYGTFATLPILLLWIYMAWVIVLL
                     GAVVAAYLPSLLAGVARRAGGQGWPFLLAVEALQQLHGARGQAAHGLTAAQLAQRLGV
                     EVLQLEPVLDALTALDWVVQVNEAATGAPGSVDARYVLLADPATTHMEPLVQRLLLTR
                     TEALERFWGHTGLEVLRLADVLERPQAPGMPPQSLLKK"
     gene            1876..2304
                     /locus_tag="PK518_14540"
     CDS             1876..2304
                     /locus_tag="PK518_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HRM50175.1"
                     /translation="MQSETAPPPSPILPDVSTDVAATRWLAVGSLLGLILLCLAWELW
                     LAPLRPGGTWLALKALPLALPLAGLLKRRMYTYRWVSLLVWLYFTEGVVRAWSDPAPG
                     NWLAGIEILLCLLLFTACALHVRLRLAAAKASTQPATEPT"
     gene            2301..3737
                     /locus_tag="PK518_14545"
     CDS             2301..3737
                     /locus_tag="PK518_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRM50176.1"
                     /translation="MTTALLDTLRQIVGAAHVLTHEDPTTDLTAYEQDWRKRATGRAL
                     AVVRPASTAEVAAVVKACAAARTAIIPQGGNTGLSVGGTPDASGTQVVLSLTRMNQVR
                     AIDRDNLTMTVEAGCILQTLQETADKAGLLFPLSLAAEGSCTIGGNLGTNAGGTQVVR
                     YGNARELCLGLEVVTPQGEVWDGLSGLRKDNTGYDLRNLFIGSEGTLGVITAATMKLY
                     PQPAAQLTAWAAVPEMAAAVRLLGLAHQHLGAGLTGFEVMGQFALSLVIKHMPQLRVP
                     FAGLKGAPPYYVLLENSDSESEQHARERFETLLELAFEDGCVLDAVVAESLAQAHELW
                     HIRESIPLAQAEEGLNIKHDISIAASRIPAFVEHTDALLAREIAGVRLVNFGHLGDGN
                     LHYNVQAPEGGDARDFLATQEDRVNQLVYDAVAQFGGSFSAEHGVGQLKADKLAHYKS
                     PVALSMMRAIKQALDPQGLMNPGCLLQK"
     gene            3933..>4152
                     /locus_tag="PK518_14550"
     CDS             3933..>4152
                     /locus_tag="PK518_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRM50177.1"
                     /translation="MTPPPIRSQYEDFMRHVFEHGVSKGDRTGTGTRSVFGHQMRFDL
                     SEGFPLITTKKVHLRSIIQELLWFLTGSS"
BASE COUNT          711 a         1496 c         1367 g          578 t
ORIGIN      
        1 cgcagcagcg cggcagcgac aacaaggccc tggctgtggg cacatcggcc ggtgccgccg
       61 ccggtgccgc cgccggcgcc aaggtgggcg cgatgatcga tgtgggcacg ggcggcctga
      121 ccctgggcct gggcacggcc ctgggcgcgc tgctgggcgg cggcaccgcc tgggcagtgc
      181 gcaatctgca aaagaaggac gcgcagctgg agggcgcgca agacctgctg cagcacatgg
      241 ccgaggccgc ctgcacgcgc tacctggcgc tggcccacct ggggcgcgtg ccggccgagc
      301 aggcgcagga actgggcgcg cgctggcagg ccgaggtcac cggcacggtg gccgcgcatt
      361 ggagcgagct gaacacagcc ctgcgcgccg atcccccaac cagcgacccg ctgcagccgc
      421 tgctgggcag catgctgcgc ggcatcttgc agcgcagctt tgtcgccgag gcgccctgat
      481 gggaacgccg gcaccttgcg ggcaagatgc caaaatatta atcaaatagg cctctagcgc
      541 ttacccatca agcgtaatca gctatttttt caatagtgac tgtggcggca tgccaggcgc
      601 ctgcggccgc tccagcacat cggccagccg cagcacctcc aggccggtat ggccccaaaa
      661 gcgctccagc gcctcggtgc gcgtgagcag caggcgctgc accagcggct ccatgtgcgt
      721 cgtggcgggg tcggccagca gcacatagcg cgcatccacc gagccgggcg cgccggtggc
      781 ggcctcattc acctgcacca cccagtcgag ggccgtgagc gcgtccagca ccggctccag
      841 ctgcagcacc tccacgccca ggcgctgggc cagctgcgcg gcggtgaggc catgcgcggc
      901 ctgaccgcgc gcgccgtgca gctgctgcag cgcctccacg gccagcagga agggccaacc
      961 ctgcccgccg gcgcgccgcg ccacgcccgc cagcagactg ggcagatagg cggccaccac
     1021 cgcgcccagc agcacgatga cccaggccat gtagatccac agcagcagga tgggcagggt
     1081 ggcaaaggtg ccgtagatca ccgaataggt gggcaccttg gccaggtaca ggcccaggcc
     1141 ctttttggcc agctccatgg ccagcgacac gaacaggctg ccggcccagg cgtcgcgcca
     1201 gtgcacctgg gtgttgggca catagcggta catggcggcc acgccggcgg ccagcagcaa
     1261 aaattcgatc gcgtccagca ataggcgtgc gccgccgggc agcgtggcgc ccagctcgcg
     1321 cgaggcggcg ccggcgatgg ccgtggtcag cgccaggctg gccgccagca ccagcgggcc
     1381 cagggtcatc gcggcccagt agatcagcac gcgctgtccc agcgggcgca gccggcgcac
     1441 gcgccagatg ccgttgagcg tgcggtctat cgtcaatatc agcgccagcg ccgtcaccat
     1501 caagatggaa aagcccgccg cccccagccc cccggccttg gccgcgaact gcgtcagata
     1561 gcccagcacc gggcgcgaga tgctgtcggg caccaggctg tcgatgagcc agcgctgcag
     1621 catcagctgc atcttgccga agatgggaaa ggccgcgaac accgccagcg ccacggtaaa
     1681 gaacggcacc agcgccagca gcgtggtaaa ggtgaggctg ctggcggtga cgcccagccg
     1741 gtcctcgcga aagcgctcgc gcagcatatg ggccgtggta cgccagggga aatgcgacag
     1801 ttgaacccac cacgcctcaa ggctctgcgc agtggcatta agggaaaacg ccatggccga
     1861 tatgatgcca gccccatgca gtcagaaacc gcccccccgc cctctcccat cctccccgat
     1921 gtcagcaccg atgtcgccgc cacgcgctgg ctggccgtgg gcagcctgct cggcctgatc
     1981 ctgctctgcc tggcctggga gctgtggctc gcccccctgc ggcccggcgg cacctggctg
     2041 gccctcaagg ccctgccgct ggccctgccg ctggcggggc tgctcaagcg ccgcatgtac
     2101 acctaccgct gggtcagcct gctggtgtgg ctgtacttca ccgaaggcgt ggtgcgcgcc
     2161 tggagcgacc cggcccccgg caactggctg gccggcatcg aaatcctgct gtgcctgctg
     2221 ctgttcaccg cctgcgccct gcatgtgcgc ctgcgcctgg cggcggccaa ggcatctacc
     2281 caacctgcca cggagcccac atgaccaccg ccctgctcga caccctgcgc cagatcgtcg
     2341 gcgccgccca tgtgctgacg cacgaagacc ccaccaccga cctgacggcc tatgagcagg
     2401 actggcgcaa gcgcgccacc ggccgcgccc tggccgtggt gcgcccggcc tccaccgccg
     2461 aggtggccgc cgtggtcaag gcctgcgcgg cggcgcgcac tgccatcatc ccccagggcg
     2521 gcaacaccgg cctgtcggtg ggcggcacgc ccgacgccag cggcacccag gtggtgctga
     2581 gcctgacgcg catgaaccaa gtccgcgcca tcgaccgcga caacctgacc atgaccgtgg
     2641 aggccggctg catcctgcag accctgcagg aaacggcgga caaggcgggc ctgctgttcc
     2701 ccctgagcct ggccgccgag ggcagctgca ccataggcgg caacctgggc acgaatgccg
     2761 gcggcaccca ggtcgtgcgc tacggcaacg cgcgcgagct gtgcctgggc ctggaggtgg
     2821 tcacgcccca gggcgaggtg tgggacggcc tgtccggcct gcgcaaggac aacaccggct
     2881 acgacctgcg caacctgttc ataggcagcg agggcacgct gggcgtgatc accgccgcca
     2941 ccatgaagct ctatccgcag cccgcggccc agctcacggc ctgggccgcc gtgccggaga
     3001 tggcggccgc cgtgcgcctc ttgggcctgg cacaccagca cctgggcgcg gggctgacgg
     3061 gcttcgaggt catggggcag tttgcgctgt cgctggtgat caagcacatg ccccagctgc
     3121 gcgtgccgtt cgccggattg aagggcgcgc cgccctacta cgtgctgctg gaaaacagcg
     3181 acagcgagtc cgaacagcat gcgcgcgagc gcttcgagac gctcctggag ctggcgtttg
     3241 aagacggctg cgtgctcgac gccgtggtgg ccgagagtct ggcccaggcg cacgagctgt
     3301 ggcatatccg cgagagcatc cccctggccc aggccgagga aggcctgaac atcaagcacg
     3361 acatatcgat tgccgcctcg cgcatcccgg cctttgtgga gcacaccgac gccctgctgg
     3421 cgcgcgagat tgccggcgtg cgcctggtca acttcggcca cctgggcgac ggcaatctgc
     3481 actacaacgt gcaggcgccc gagggcggcg acgcgcgcga tttcctggcc acgcaggagg
     3541 atcgcgtgaa ccagctggtc tatgacgcgg tggcgcagtt cggcggctcc ttctcggccg
     3601 agcatggcgt gggccagctc aaggccgaca agctggcgca ctacaagtcg cccgtggccc
     3661 tgtcgatgat gcgcgccatc aagcaggcgc tggacccgca gggactgatg aaccccggct
     3721 gcttactaca aaaataatag cttactccgc ttgacagata agcgctatat ccacttttaa
     3781 taccagaatt gtcatgctgt gcaagcccct cacccctccc tctccccaaa ggggcgaggg
     3841 cgaccggtcg cgccccggtc tttctccctc tccctctggg cagagggcgg gggtgagggc
     3901 cagccaccct aatcccaaac atgccaccca ccatgacccc accccccata cgcagccaat
     3961 acgaagactt catgcgccat gtgttcgagc atggcgtcag caaaggcgac cgcaccggca
     4021 ccggcacgcg cagcgtcttc ggccaccaga tgcgctttga cctctcggaa ggctttccgc
     4081 tgatcaccac caagaaggtg cacctgcgct ccatcatcca ggagctgctg tggttcctga
     4141 ccggctccag ca
//
LOCUS       DAOMTQ010000211         4116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5823_length_4116_cov_27.608225, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000211 DAOMTQ010000000
VERSION     DAOMTQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5823_length_4116_cov_27.608225"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK518_14555"
     CDS             <1..228
                     /locus_tag="PK518_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014816.1"
                     /note="catalyzes the formation of malonyl-CoA from
                     malonate and CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HRM50178.1"
                     /translation="LVGVPHPDFGEVGVAVVIAKPGAQLDGEAIITALKSQLANFKIP
                     KRCFVATELPRNTMGKVQKNLLREQHKGLFA"
     gene            complement(340..783)
                     /locus_tag="PK518_14560"
     CDS             complement(340..783)
                     /locus_tag="PK518_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM50179.1"
                     /translation="MYKRILIATDGSQLSDMAVESGLSLAGLTGASVIALKVVPRYPR
                     SYFDGTLPVEAADIKRIEKQWSDAAQVLVNKVKARGSDEGVSVKAVVVKSDLIAEAVI
                     AAAKKHNCDLIVMASHGRKGIKRLLLGSETQHVLTHSHIPVLVLR"
     gene            896..3271
                     /locus_tag="PK518_14565"
     CDS             896..3271
                     /locus_tag="PK518_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM50180.1"
                     /translation="MSNKPAGQLAATDTAMLLDDPQEWSAFGRPHGRAAADMPPTQTA
                     WESHVVLEGMHCTACALTIEDALRQVPGVSDVDVSAATRRARVVWNPAQVLPSQWMEA
                     VRRAGYRSIPAMDALAREERRRENRRALWRWLVAGFCMMQVMMYAWPAYQAMPGDLTF
                     EMEQLLRWASWVISLPVVLFACGPFFSSALRDLRQRTVSMDLPVALGMLITFVVSMLG
                     TFDPAGPFGREVYYDSLTMFVFFLLTGRWLELRLRDRTAGALEAVMNRLPDSVLRRTA
                     AGDFERVATRRLKLGDVLRVLPGEAFPADGRILNGSTQADEALLTGESTPVAKQPASP
                     VTAGSYNLSAAVEVVVEALGAQTRFGQIVTLMESASLQKPRLAQLADRVARPFLVGVL
                     LAAALAAAWWWPTDPSHALMVAVAVLVVTCPCALSLATPVAMLTAAGTLARSGVLVRN
                     LQGLEALAAVDTVVFDKTGTLTRDRLAVTGTQLGPDAGLTAMQALALAARLAQHSLHP
                     VARAVVAAAEVLPPHDDSWLVGAVEELPGAGLQALGSRNGQQGEAILRLGSVAHARVA
                     PAEGAPQQVVLSAEREGRVTELARFDLSEELRPEAPAVVQQLRDAGIAVELLSGDHGL
                     AVQRVAQRTGIDAAQGACTPQDKLQHLQALQAQGHLVAMVGDGLNDGPVLAGAHASLA
                     FGKSVALAQSRSDIVVLGDNLALVAQTLLLARRTMHIVRQNLWWAAGYNAVGVPLAIA
                     GYMPAWLAGLGMALSSLLVVLNAARLARTAGGDAPANAHAPLSAQGPVGVG"
     gene            3274..3411
                     /gene="ccoS"
                     /locus_tag="PK518_14570"
     CDS             3274..3411
                     /gene="ccoS"
                     /locus_tag="PK518_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRM50181.1"
                     /translation="MDILYLLIPLSVVLVLFVLAGLWWAVYRGQFENVEQEGERILRG
                     D"
     gene            3477..>4116
                     /locus_tag="PK518_14575"
     CDS             3477..>4116
                     /locus_tag="PK518_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRM50182.1"
                     /translation="MESSQTKVAHYDDTVVRQFSIMAVVWGVVGMLVGVFIAAQLTWP
                     ELNFGIPWLSYGRLRPLHTNAVIFAFGGCGLFATSYYVVQRTCQTKLFGGPLIPFTFW
                     GWQIVIVAAAISLPLGYTTSKEYAELEWPIDILIALVWVSYAIVFFGTVGKRKVKHIY
                     VANWFFGAFILAVALLHIVNSAEMPAGWMKSYSAYAGVQDAMVQWWYGHNAVG"
BASE COUNT          632 a         1274 c         1400 g          810 t
ORIGIN      
        1 ctggtgggcg tgccgcaccc ggattttggc gaggtgggcg tggcggtggt catcgccaag
       61 cccggcgccc agctcgacgg cgaggcgatc atcaccgcac tcaagagcca gctggccaac
      121 ttcaagatcc ccaagcgctg cttcgtcgcc accgaactgc cacgcaacac catgggcaag
      181 gtgcagaaga acctgctgcg cgagcagcac aagggcttgt ttgcctgagg gcgccgcgcc
      241 cagaaaaacc aaaggcggcc agggccgcct ttgctcaaat actatataaa taatagctgc
      301 ttgcgcccgt atccagggcg ctggcggctt gttttgcctt cagcgcagca ccagcaccgg
      361 gatgtgtgaa tgcgtcagca catgctgggt ttcgctgccc agcagcaggc gtttgatgcc
      421 cttgcggccg tgcgaggcca tgacgatcag gtcgcagttg tgcttcttgg cggcggcgat
      481 cacggcctcg gcaatcaggt cggacttcac caccacggcc ttgacgctca cgccctcgtc
      541 cgagccgcgc gccttgacct tgttcaccag aacctgggcc gcatcgctcc attgcttctc
      601 gatgcgcttg atgtccgctg cctcgaccgg cagggtgccg tcgaagtagc tgcgcgggta
      661 gcgcggcacc accttcaggg caatcaccga tgcgcccgtc aggcctgcca gcgacaggcc
      721 actctccacg gccatgtccg acagctgtga accgtccgtg gcgatcagta tgcgtttgta
      781 catgagctgc tcctaatatt tggttgaaac ggcgaaccag cataggatag cccacggcgc
      841 tcgtcttgat gcatatcaat ttgagccttg acgcaatacg gataattcgc gatctatgtc
      901 aaacaaaccc gccggccagt tggcggccac cgataccgcc atgctgctgg acgaccctca
      961 ggaatggtcg gccttcggtc ggccgcatgg ccgtgcggcg gcggatatgc caccgacgca
     1021 gaccgcctgg gaatcccatg tggtgctgga gggcatgcat tgcacggcct gcgccctgac
     1081 catagaggac gccttgcgcc aggtgcccgg cgtcagcgac gtggacgtga gcgccgccac
     1141 gcgccgcgcg cgcgtggtgt ggaacccggc gcaggtgctg ccatcgcaat ggatggaggc
     1201 cgtgcgcagg gccggctacc gatccatccc cgccatggac gcgctggcgc gcgaggagcg
     1261 ccggcgtgag aaccgccgcg ccctctggcg ctggctggtc gcgggttttt gcatgatgca
     1321 ggtcatgatg tacgcatggc ccgcctacca ggccatgccc ggtgacctga cgttcgagat
     1381 ggagcagctg ctgcgctggg cttcctgggt catcagcctg cccgtggtgc tgtttgcctg
     1441 cgggccgttc ttctccagcg cgctgcgcga tctgcgtcag cgcaccgtga gcatggatct
     1501 gccggtggcg ctgggcatgc tgatcacctt tgtggtgagc atgctgggca ccttcgaccc
     1561 cgccgggccg ttcgggcgcg aggtgtatta cgactcgctg accatgttcg tgttcttttt
     1621 gctcactggc cgttggctgg agctgcgcct gcgcgaccgc acggcggggg cgctggaagc
     1681 ggtcatgaac cgcctgcccg acagcgtgtt gcggcgcacg gcggcgggag actttgaacg
     1741 cgtggccacg cggcgcctga agctgggcga tgtgctgcgg gtgctgcccg gcgaggcttt
     1801 cccggccgat gggcgcatcc tgaatggctc cacccaggcc gacgaggcat tgttgacggg
     1861 tgagtccacg ccggtggcca agcagcccgc cagcccggtg acggccggca gctataacct
     1921 gagcgcggcg gtcgaggtgg tggtggaggc actgggcgca cagacacgtt ttggccagat
     1981 cgtcacgctg atggaaagcg cctcgctgca aaaaccccgc ctggcgcagc tggccgaccg
     2041 tgtcgcgcgg ccgtttctgg tcggcgtgct gcttgctgcg gcgctggccg ccgcctggtg
     2101 gtggcccacc gatcctagcc atgcgttgat ggtcgccgtc gcggtgctgg tggtcacctg
     2161 cccctgtgcg ctgtcgctgg ccacgccggt ggccatgctc accgctgcag gcacgctggc
     2221 gcgcagcggc gtgctggtgc gcaacctgca ggggctggag gctctcgccg ctgtggatac
     2281 cgtggtgttt gacaagaccg gcacactcac gcgcgaccgg ctggcggtta ccggtacgca
     2341 gctggggcct gatgccgggc ttacggccat gcaggctctg gccctggcgg cccggctggc
     2401 gcagcattcc ctgcatccgg tggcgcgtgc agtggtggcc gcggccgagg tcttgccgcc
     2461 gcatgacgac tcctggcttg tgggcgcggt ggaggagttg ccgggcgcag gcctgcaggc
     2521 gcttgggagc cggaatggac agcaggggga ggcgatcctg cgcctggggt cggtagcgca
     2581 tgcgcgtgtg gcgcccgcag aaggtgcgcc acagcaggtc gtgctgtcgg ctgagcggga
     2641 aggccgggtt accgaactgg cgcgctttga cttgagcgaa gaactgcgcc ccgaggctcc
     2701 agccgtggtg cagcagctgc gcgacgcggg gatcgccgtg gagctgttgt ctggcgacca
     2761 tggcctggct gtgcaacgcg tggcacagcg cacgggcatc gatgcggcgc aaggggcctg
     2821 cacaccgcag gacaagctgc agcatctgca ggctctgcag gcgcaggggc acctggtggc
     2881 catggtgggt gatggcctca atgacggtcc ggtgttggct ggcgcacacg cttcgctggc
     2941 ctttggcaag tctgttgcgt tggcgcaatc ccgttctgac atcgttgttt tgggcgacaa
     3001 tctcgccctc gtagcccaga ccctgctatt ggcgcggcgc accatgcaca tcgtgcgcca
     3061 gaacctgtgg tgggctgctg gctacaacgc cgtgggtgta cccctggcca ttgccggtta
     3121 catgccggcc tggctggcag ggctgggcat ggcgctcagc tcgctgctgg tggttttgaa
     3181 tgccgcgcgc ctggcgcgta ccgcgggtgg tgatgctccc gccaacgccc acgcgccgct
     3241 ctcggcgcaa gggcctgtgg gggtgggttg accatggaca ttttgtatct gttgattcct
     3301 ctatccgtcg tgcttgtgct gtttgtgctg gcgggcctgt ggtgggcggt ctaccgagga
     3361 caatttgaaa acgtcgaaca agagggagag cgcatattgc ggggcgattg acaatagtca
     3421 ataaccctag tgcgagtctg gttgaaactt ttttctgctt agcaacgagg taaccgatgg
     3481 agtcatcaca aacaaaagtt gcgcactacg acgataccgt cgtgcgccag ttctcgatca
     3541 tggcggtcgt ctggggggtg gtcggcatgc tggtcggcgt gttcatcgcc gcccagctca
     3601 catggcccga gcttaatttt ggtattccat ggctgagcta tggccgcctg cgcccactgc
     3661 acaccaatgc ggtgattttt gcctttggcg gttgcggcct gttcgctacc agctactacg
     3721 tggtgcagcg cacctgccag accaagctgt ttggtgggcc gctgatcccg tttaccttct
     3781 ggggttggca aatcgtcatc gtggccgccg ccatctccct gccgctgggt tacaccacga
     3841 gcaaggagta cgccgagctg gaatggccta tcgacatcct gatcgccctg gtctgggtgt
     3901 cctatgccat cgtgttcttc ggcaccgtcg gcaagcgcaa ggtcaagcac atctatgtgg
     3961 ccaactggtt ctttggcgca ttcatcctgg ccgtggccct gctgcacatc gtcaacagcg
     4021 cggagatgcc cgctggctgg atgaagagct actccgccta cgcgggcgtg caggatgcca
     4081 tggtgcagtg gtggtatggc cacaatgccg tgggct
//
LOCUS       DAOMTQ010000212         4114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5830_length_4114_cov_25.581670, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000212 DAOMTQ010000000
VERSION     DAOMTQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5830_length_4114_cov_25.581670"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK518_14580"
     CDS             complement(<1..586)
                     /locus_tag="PK518_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM50183.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFL
                     TRYDPREVACLIVDIRMGGMTGLELQDRLLERNSPLPIVFITGHGDVPMAVNTMKKGA
                     LDFIQKPFDEQELLGLVERMLDHAREAFTDHQQAASRDALLSKLTGREAQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLNANTVAD"
     gene            complement(583..3138)
                     /locus_tag="PK518_14585"
     CDS             complement(583..3138)
                     /locus_tag="PK518_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRM50184.1"
                     /translation="MATPSPSPSSPVSAIKTPARWWRAWWRSLSPMRQDRFATLAPLA
                     AVLMFLAAIVASFWYLRSEEIAREKEALKRDVEYAQQRVRLRLLERQEQIMRIARDLS
                     NEELERADFVSRAEALISQYPELQSITWIDERRRIRASQAAPTVTSSQLRVTGEVLKT
                     GEAADLFKQARQLRQPVYSLPTAGAGDTAPLLQLQVPLSPHDQFGGVVLAEYSIDSLL
                     RYGTPTEVLARYAVTLLDGRGQVLAGTPLAPRHNALLPWTTKANAYASPVSPVGNGLV
                     LRAQAYRTSLGVVGSGLFWLVGSLSVMTSWLLIATWRHTRRRMRAQEALIVETNFRRA
                     MENSILTGMRALDLQGRITYVNSAFCQMTGWTEAELVGQTPPYSYWPEGEYDKLHANL
                     RDELSGKTVPGGFQVRVRRKSGTLFDARLYVSPLIDARGQQTGWMTSMTDITEPNRVR
                     EQLTASHERFTIVLEALDASVSVAPLGSQELLFANRLYRQWFGSQTGGHLEMVAQAGV
                     PPSPSAPSGDDVGAEDDLLGLPVDPLARARAENAEIYRSDLGKWLEVRSRYLNWVDGR
                     LAQMVIATDITPRRVAEEQAARQAERAQSVSRLITMGEMASSVAHELNQPLTAINNYC
                     SGMVSRIQNGTLSEEALLTALQKTAHQAQRAGQIIQRIRAFVKKSEPNRTLANVHDMV
                     GEALELAEIELRRHNVRLTHHVAARLPPVMADSILIEQVLINLMKNGAEAIAQAQRPP
                     ALRSVELRVVPRQVDGAEVIEFSVQDTGRGLAPEVLAHLFEAFFSTKSEGMGIGLNLC
                     RSIVESHQGRMAAENLYNATEVTGCRFSFWLPLANTGDSAITSAATTYPRTTA"
     gene            3313..>4114
                     /gene="aceE"
                     /locus_tag="PK518_14590"
     CDS             3313..>4114
                     /gene="aceE"
                     /locus_tag="PK518_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805356.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRM50185.1"
                     /translation="MSDPTKAKTAADIDQEETREWLEALSAVIEKEGPERAHFLLEQL
                     LEHGRQSSIDLPFSANTGYVNTIEPEQEARCPGNIAIEKRLRAYMRWNAMAMVVRANR
                     LHPADGGDLGGHIGSFASLASMWGAGFNHFWHAESETHGGDCIYFQGHSAPGIYARAF
                     LEGRISEDQLENFRQEVDGKGLSSYPHPKLMPGFWQFPTVSMGLGPMMAIYQARFLKY
                     LHARGIADTAKRRVWVFLGDGEMDEPESKGAISLAARENLDNLIFVINC"
BASE COUNT          729 a         1294 c         1325 g          766 t
ORIGIN      
        1 ggtcggccac ggtgttggcg ttgagctttt ccatgatgtt ggcgcggtgc gcctcgacgg
       61 tcttgatgct gatgcccaga tcgtcggcga tctgcttgtt caagcggccg gcgacgatgc
      121 gctcgagcac ctgggcctcg cggccggtga gcttggacag aagcgcatcg cggctggcgg
      181 cctgctggtg gtcggtgaag gcctcgcggg catgatcgag catgcgctcc accaggccca
      241 gcaactcctg ctcgtcaaac ggcttctgga tgaaatccag ggcgcccttc ttcatggtgt
      301 tgacggccat gggcacgtcg ccatggccgg tgataaagac gatgggcagg ggcgagttgc
      361 gttccagcag cctatcttgc agctccagcc cggtcatgcc gcccatgcgg atgtccacga
      421 tcaggcaggc cacttcacgc gggtcgtagc gggtcaggaa agactctgcc gaatcgaagc
      481 accgaacgcg gtaatccttg ccctccagca gccactgaag tgaatcgcgg acggcttcat
      541 cgtcatcgac gacatagacg gtaccttttt tcggaatcaa gctcatgcag tagtccttgg
      601 atatgtcgtt gctgctgaag taatagcact atcgcctgta ttggcgagtg gcaaccagaa
      661 agaaaaccga caaccggtaa cctctgttgc attgtagagg ttctccgcgg ccatccttcc
      721 ctggtgcgat tcgacaatgc tgcggcacaa atttaagcct atccccatac cctcgctctt
      781 ggtggagaaa aaggcctcga acaaatgcgc cagcacttcc ggcgccaggc ccctgccggt
      841 gtcctgtacc gagaattcga tgacctcggc gccatccacc tggcgtggta ccacgcgcag
      901 ctccacgctg cgtagcgccg gcgggcgctg cgcctgggcg atggcctcgg cgccattctt
      961 catcaggttg atgagcacct gttcaatcaa tatggagtcc gccatgaccg gtggcaggcg
     1021 ggccgcgaca tggtgtgtca ggcgcacgtt gtggcggcgc agctctattt cggccaactc
     1081 caacgcttcg cccaccatgt cgtgcacgtt ggccagcgtg cgattgggct cgcttttctt
     1141 cacgaaggcg cgaatgcgct ggatgatctg gccggcgcgc tgggcctggt gcgcggtctt
     1201 ttgcagcgcg gtcagcagag cctcctcgct gagggtgccg ttctggatgc gcgagaccat
     1261 gccactgcag tagttgttga tcgcggtgag cggctgattg agctcgtgcg ccacgctcga
     1321 cgccatctcg cccatggtga tcagtcggct gaccgactgg gcgcgctcgg cctggcgtgc
     1381 agcctgttcc tcggccacgc gccgcggggt gatgtcggtg gcgatgacca tctgcgccag
     1441 ccggccatcg acccagttca ggtagcgcga gcgcacttcc agccatttgc ccaggtcgct
     1501 gcggtagatc tcggcgtttt ccgcgcgggc cctggccagc ggatccaccg gcaggcccaa
     1561 caggtcgtcc tctgccccca cgtcgtcacc acttggcgca gacggagagg gcggaacacc
     1621 cgcctgcgcc accatctcca ggtgccctcc ggtctgcgag ccaaaccact ggcggtacag
     1681 gcggttggca aacagcagct cctggctgcc cagcggggcg accgacaccg atgcatccag
     1741 cgcctccagc acgatggtga agcgctcgtg tgaggcggtg agctgctcgc gcacgcggtt
     1801 gggctcggtg atgtccgtca tcgatgtcat ccagccggtc tgctggccgc gcgcgtcaat
     1861 cagcggtgag acgtacaggc gcgcatcgaa cagggttccg ctcttgcgtc gcacgcgcac
     1921 ctgaaagccg ccgggcacgg tcttgcccga gagctcgtcg cgcaggttgg cgtgcagctt
     1981 gtcgtattcg ccctcgggcc agtagctgta gggcggtgtc tggcccacca gctcggcctc
     2041 ggtccagccc gtcatctggc agaaggcgga gttcacgtag gtgatgcgcc cttgcagatc
     2101 cagggcgcgc atgccggtca ggatggagtt ttccattgcc cggcggaaat tggtctcgac
     2161 gatcaaggcc tcttgcgcgc gcatgcgccg gcgcgtgtgg cgccaggtgg cgatcaacag
     2221 ccatgacgtc atgacgctca agctgcccac cagccagaac aggccactgc ccaccacgcc
     2281 cagcgaggtg cggtaggcct gggcgcgcag taccaggcca ttgcctaccg gcgagacggg
     2341 gctggcatag gcgttggcct tggtcgtcca gggcagcagg gcgttgtgcc tgggcgccag
     2401 aggcgttccg gccagaacct ggccacggcc gtcgagcagg gtcacggcgt agcgcgccag
     2461 aacctccgtg ggcgtgccgt agcgcagcag gctgtcgatg gagtattcgg ccagcaccac
     2521 gccgccaaac tggtcatgcg gcgatagcgg cacctgcaat tgcagcagtg gggcggtgtc
     2581 gcctgctcct gccgttggca gtgaatacac gggttgccgc aactggcgcg cctgcttgaa
     2641 caaatcagct gcctcgcccg tcttcagcac ctcgccggtc acgcgcaact ggctgctggt
     2701 cacggtgggg gcggcctggc tggcacggat acggcggcgc tcgtcgatcc aggtgatgga
     2761 ctgcagctcg gggtactggc tgatcagggc ctcggcgcgg ctcacgaagt cggcgcgttc
     2821 cagttcttcg ttggacagat cgcgcgcgat gcgcatgatc tgctcctggc gctccagcag
     2881 gcgcagtcgt acgcgctgct gggcgtattc cacgtcgcgt ttgagggcct ccttttcacg
     2941 ggcgatctct tcggagcgca aataccagaa agaagccacg atcgccgcca ggaacatcag
     3001 caccgccgcc agcggggcca gcgtggcaaa gcgatcctgg cgcatcggcg acaggctgcg
     3061 ccaccatgcg cgccaccagc gcgccggcgt ttttatcgca gacacgggtg agctgggcga
     3121 ggggctgggg gtggccatgg agtggagttt aaggccgcgc aggagctgtt ttcattgtga
     3181 aaaatgatgt gcatctgcag caaaatttca taataagaaa tcaacagccg ctatttgaaa
     3241 ttttgtcgaa ctatgcgaaa cttgcgcccg taattcgttc accatagtca ctcacttcaa
     3301 aggagacagg gcatgtccga tccgaccaaa gccaagactg ctgccgatat agaccaagag
     3361 gaaacccgcg aatggctgga ggcgctgtcc gcggtcattg agaaagaggg cccggagcgg
     3421 gcgcactttc tgctggagca gctgctcgaa catggtcggc aaagcagcat cgacctgccg
     3481 ttctcggcca acaccggcta tgtgaacacc atcgagcccg agcaggaggc gcgctgcccc
     3541 ggcaacatcg ccatcgaaaa acgcctgcgc gcctatatgc gctggaacgc catggccatg
     3601 gtggtgcgtg ccaaccgcct gcacccggcc gatggtggcg acctgggcgg gcacatcggc
     3661 tcgtttgcct cgctggcttc catgtggggc gcgggcttca accatttctg gcatgccgag
     3721 agcgaaaccc acggcggcga ctgcatttac tttcagggcc atagcgcgcc cggcatctat
     3781 gcccgcgcct ttctggaggg ccgtatcagc gaagaccagc tggagaactt ccgccaggaa
     3841 gtggacggta aaggcctgtc aagctacccc caccccaagc tgatgccggg cttctggcag
     3901 ttccccaccg tgtccatggg cctgggcccg atgatggcca tctaccaggc acgctttttg
     3961 aaatacctgc acgcccgcgg cattgccgac acggccaagc gccgcgtctg ggtgttcctg
     4021 ggtgacggcg agatggacga gcccgagagc aagggcgcga tcagcctggc ggcgcgcgag
     4081 aacctggaca acctgatctt cgtcatcaac tgca
//
LOCUS       DAOMTQ010000213         4072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5920_length_4072_cov_18.982574, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000213 DAOMTQ010000000
VERSION     DAOMTQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5920_length_4072_cov_18.982574"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PK518_14595"
     CDS             complement(<1..834)
                     /locus_tag="PK518_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRM50186.1"
                     /translation="MSDLSLQLQQAASQLPVSSYFDAALLQRERELLFQQGPRYVGHA
                     HAIPEPGDYYVLPQEKGGRALVRKPDGSIELLSNCCRHRQAIMLQGRGNLNTEGPGHA
                     GGNIVCPIHRWTYSCSGELLGAPHFAEDPGLHLPNYALREWNGLLFEDNGRDIAADLA
                     GMGVASELSFDGYVLDRTEMHECNYNWKTFIEVYLEDYHVGPFHPGLGNFVTCDDLQW
                     QFAKDYSVQTVGVAPSFGKPGSAVYKKWHEVLLKYQGGELPRRGAIWLTYYPHIMVEW
                     YP"
     gene            1096..1338
                     /gene="xseB"
                     /locus_tag="PK518_14600"
     CDS             1096..1338
                     /gene="xseB"
                     /locus_tag="PK518_14600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRM50187.1"
                     /translation="MPKAAAAPAPQSEPASYEAALEELEQLVARIESGQLPLEQMLAG
                     YQRGAVLLNFCRARLDAVQDQIKVLDEGALQPWTQE"
     gene            1338..2231
                     /locus_tag="PK518_14605"
     CDS             1338..2231
                     /locus_tag="PK518_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRM50188.1"
                     /translation="MHTADHDFDGWMREQLARVESGLSLWVRRDAPAGLGEAMRYAVL
                     DGGKRLRPLLVLAACAAVDGHQEAALRAACAVELIHAYSLVHDDMPCMDNDVLRRGKP
                     TVHVRFGQAQALLAGDALQALAFELLTPEDNVPPAMQASLCRLLAQAAGADGMAGGQA
                     IDLASVGRGLSEAELRHMHRLKTGALLQASVVMGAACGAPGQQAAQALAQYGAALGLA
                     FQVMDDVLDVTQDSATLGKTAGKDAASDKPTYVSLMGLAPAQAYAEALRTQAHAALAA
                     SGLPDTRALAALADMVVRRSH"
     gene            2304..>4072
                     /gene="dxs"
                     /locus_tag="PK518_14610"
     CDS             2304..>4072
                     /gene="dxs"
                     /locus_tag="PK518_14610"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRM50189.1"
                     /translation="MSTMNYPLLERVNDPADLRCLPRAELKSLAHELRAFVLESVSKT
                     GGHLSSNLGTVELTVALHAVFDTPRDRLVWDVGHQTYPHKILTGRRERMPSLRQLGGL
                     SGFPQRAESEYDTFGTAHSSTSISAALGMALAARQKGEDRHAIAIIGDGAMTAGMAFE
                     ALNNAGVADCNLLVILNDNDMSISPPVGALNRYLAQLMSGQFYATAKGVGKSVLKNVP
                     PLLELAKRLEQQAKGMVVPATLFEKFGFNYIGPIDGHDLDSLIPTLENIKGLKGPQFL
                     HVVTRKGQGYKLAEADPVAYHGPGKFDPKIGLVKPATPAKQTFTQVFGQWLCDMAAKD
                     QRLVGITPAMREGSGMVEFHQRYPGRYYDVGIAEQHAVTFAAGMACEGVKPVVAIYST
                     FLQRAYDQLIHDVALQNLPVVFALDRAGLVGADGATHAGAYDIAFVRCIPNMSLACPA
                     DERECRQLLTTAYEQDHPVAVRYPRGSGAGVAPLASLEGLPFGKGEVRRQGRRIAILA
                     FGTLLYPALQAAEKLGATVVNMRWAKPLDAELLLRVAAEHEALVTVEEGCIMGGAGSA
                     VAEALNAAGVQRPLLQLGLPDTF"
BASE COUNT          675 a         1386 c         1381 g          630 t
ORIGIN      
        1 cgggtaccac tcgaccatga tgtgcgggta gtaggtcagc cagatggcgc cgcgcctggg
       61 caactcgccg ccctggtact tcagcagcac ctcgtgccac ttcttgtaca ccgccgagcc
      121 gggcttgccg aacgacggcg ccacgcccac ggtctgcacc gagtagtcct tggcgaactg
      181 ccactgcagg tcgtcgcagg tgacgaagtt gcccaggccg gggtggaagg ggccgacgtg
      241 gtagtcctcc agatagacct cgatgaaggt cttccagttg tagttgcact cgtgcatttc
      301 ggtgcggtcg agcacgtagc cgtcgaacga cagctcggac gccacgccca tgccagccag
      361 gtcggcagcg atgtcgcgcc cgttgtcctc gaacagcagg ccgttccact cgcgcagcgc
      421 gtagttgggc aggtgcaggc cggggtcctc ggcaaaatgc ggcgcgccca gcaactcgcc
      481 gctgcacgaa taggtccagc ggtggatggg gcagacgatg ttgccgccgg cgtggcccgg
      541 gccctcggtg ttcaggttgc cgcgcccctg cagcatgatg gcctggcggt ggcggcagca
      601 gttggagagc agctcgatgc tgccatcggg cttgcgcacc agcgcgcgcc cgcctttttc
      661 ttgcggcagc acgtagtagt cgcccggctc ggggatggca tgggcatggc cgacatagcg
      721 cggcccctgc tggaacaata gctcgcgctc gcgctgcagc agcgcggcgt cgaagtagct
      781 ggaaactggt agttggcttg cggcctgctg cagttgaaga cttaaatcag acatgggtgt
      841 cgtgacctaa agactcccca cagggagggt gcgtgtgcgc acggacaaga aaaaacgaaa
      901 ccggcccaga gaaaacccta aaagccggtg cgtgacgaag agaatgggat tatagatggt
      961 ggggttattt ccgcagctag cgtactggta ttgcatagag ggggggtgtg ggatgtgacg
     1021 gcggctgccg ccccgttttt gacgtagcgc ctaaaattac gggtttttac ttgcgcgcca
     1081 ggcgccacac cacccatgcc caaggccgcc gccgccccag ccccacagtc cgaacccgcc
     1141 agctacgagg ctgcgctgga ggagcttgag cagctcgtgg cccgcatcga gtcgggccag
     1201 ctgccgctgg agcagatgct ggccggctac caacgtggcg ccgtgctgct caacttctgc
     1261 cgcgcccggc tggacgcggt gcaggatcag atcaaggtgc tggacgaggg ggcgctgcag
     1321 ccatggacgc aggagtaatg cataccgctg accatgactt cgacggctgg atgcgcgagc
     1381 agctcgcgcg ggtcgaatcg ggcctgtcgc tctgggtgcg tcgcgacgcg cccgcgggcc
     1441 tgggcgaggc catgcgctac gccgtgctcg atggcggcaa gcgcctgcgc ccgctgctgg
     1501 tgctggccgc ctgcgccgca gtggacgggc accaggaggc tgccttgcgc gccgcctgcg
     1561 ccgtggagct gatccacgcc tattcgctgg tgcacgacga catgccctgc atggacaacg
     1621 acgtgctgcg ccgcggcaag cccacggtgc atgtgcgctt tggccaggcc caggcactgc
     1681 tcgccggcga cgcgctgcag gccctggcct tcgagctgct gacccccgag gacaacgtgc
     1741 cccccgccat gcaggccagc ctgtgccgcc tgctggccca ggccgcgggc gccgacggca
     1801 tggccggcgg ccaggccata gacctggcca gcgtgggccg cggcttgagc gaggccgagc
     1861 tgcgccacat gcaccgcctg aagaccggcg cgctgctgca ggccagcgtg gtcatgggcg
     1921 cggcctgcgg tgcgcccggc cagcaggcgg cgcaggcgct ggcgcaatat ggcgcggccc
     1981 tgggcctggc cttccaggtc atggacgacg tgctggacgt gacccaggat tcggccaccc
     2041 tgggcaagac cgccggcaag gacgccgcca gcgacaagcc cacctatgta tcgctgatgg
     2101 gcctggcccc cgcccaggcc tatgccgagg ccctgcgcac ccaggcgcac gccgcgctgg
     2161 ccgccagcgg cctgcctgac acgcgtgcgc tggcggcctt ggccgacatg gtggtgcggc
     2221 gctcgcactg ataacggcgt ttgtataaaa acggcctcta acgcttattt gataagcgct
     2281 aacagctata gaaacaagag cttatgtcca cgatgaatta cccgctgctt gaacgcgtga
     2341 acgacccggc cgacctgcgc tgcctgccgc gcgccgagct caagagcctg gcccatgagc
     2401 tgcgcgcctt cgtgctcgag agcgtctcca agaccggcgg gcatctgtcg tccaacctgg
     2461 gcacggtgga gctgacggtg gcgctgcacg ccgtgttcga cacaccgcgc gaccggctgg
     2521 tgtgggacgt gggccaccag acctatccgc acaagatcct caccggccgg cgcgagcgca
     2581 tgcccagcct gcgccagctg ggcggcctgt cgggctttcc gcagcgcgcc gagagcgagt
     2641 acgacacctt cggcacggcg cattccagca ccagcatctc cgccgcctta gggatggccc
     2701 tggccgccag gcaaaagggc gaggatcgcc acgccatcgc catcatcggt gacggcgcca
     2761 tgaccgccgg catggccttc gaggcgctga acaacgccgg cgtggccgac tgcaatctgc
     2821 tcgtcatcct gaacgacaac gacatgagca tcagcccgcc cgtgggcgcg ctgaaccgct
     2881 atctggcgca gctcatgagc ggccagttct acgccaccgc caagggcgtg ggcaagagcg
     2941 tgctgaagaa cgtaccgccg ctcctggagc tggccaagcg cctggagcag caggccaagg
     3001 gcatggtggt gccggccacg ctgttcgaga agttcggctt caactacatc ggccccatcg
     3061 acgggcatga cctggattcg ctcatcccca cgctggagaa catcaagggc ctgaagggcc
     3121 cgcagttcct gcatgtggtc accaggaagg gccagggcta caagctggcc gaggccgacc
     3181 cggtggccta tcacggcccc ggcaagttcg acccgaagat cggcctggtc aagcccgcca
     3241 cgccagccaa gcagaccttt acccaggtct tcggccaatg gctgtgcgac atggccgcca
     3301 aggaccagcg cctggtgggc atcacgccgg ccatgcgcga gggctccggc atggtcgagt
     3361 tccaccagcg ctaccctggc cgctactacg acgtgggcat tgccgagcag catgccgtga
     3421 cctttgccgc cggcatggcc tgcgagggcg tgaaacccgt ggtcgccatc tactcgacct
     3481 tcctgcagcg cgcctatgac cagctgatcc acgacgtggc gctgcagaac ctgccggtgg
     3541 tgttcgcgct cgatcgcgca gggctcgtcg gcgccgacgg cgccacgcac gccggtgcct
     3601 acgacatcgc ctttgtgcgc tgcatcccca acatgagcct ggcctgcccg gccgacgagc
     3661 gcgaatgccg ccaactgctc accaccgcct acgagcagga tcaccccgtc gccgtgcgct
     3721 acccgcgcgg cagcggcgcg ggcgtggcgc cgctggccag cctggaggga ctgcccttcg
     3781 gcaagggcga ggttcgccgc cagggcaggc gcatcgccat cctggccttc ggcacgctgc
     3841 tctacccggc gctgcaggcg gcggaaaagt tgggcgccac cgtggtcaac atgcgttggg
     3901 ccaagccgct ggatgccgag ctgctgctgc gggtggccgc cgagcatgag gccctggtca
     3961 ccgtggagga gggctgcatc atgggcggcg ccggcagcgc cgtggccgag gccctgaacg
     4021 ccgccggcgt gcagcgcccg ctgctgcagc tgggcctgcc cgacaccttc at
//
LOCUS       DAOMTQ010000214         4063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5941_length_4063_cov_27.703343, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000214 DAOMTQ010000000
VERSION     DAOMTQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5941_length_4063_cov_27.703343"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PK518_14615"
     CDS             <1..223
                     /locus_tag="PK518_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50190.1"
                     /translation="GRAPGLSCIPCLVTDHGHWQDEKAALTQLNTELDARVAARTARI
                     ERLAQDARHAAMPPCRRDARAPAPGARPA"
     gene            159..815
                     /locus_tag="PK518_14620"
     CDS             159..815
                     /locus_tag="PK518_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM50191.1"
                     /translation="MPPCRHAAVTRERLRLARGLHDTLAHSLMALLTQIRMMRKLGPS
                     WSREQLDAELADTETLAASGLVQARAAIAQMRDAGVHDNGLGPELQALLQRLAERSGL
                     RVQAHIDAAAADLVDERAAMVLAMAREALRNVERHAGAHQVSLSLAPLDAPDAGGDAP
                     APWRLVLRDDGCGFDPGAIPDGHFGLVGLREQAQQLGATLHIDSAPGQGCRVQLDFAA
                     "
     gene            complement(862..1974)
                     /locus_tag="PK518_14625"
     CDS             complement(862..1974)
                     /locus_tag="PK518_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRM50192.1"
                     /translation="MTTLFDPIRAGDLHLANRIAMAPLTRNRSPGAVPGDMAVTYYAQ
                     RASAGLLITEATAISQQGQGYSDVPGLYDSEQLQGWKKVTQAVHAQGGKIVTQLWHVG
                     RVSHCSLQPGNAAPVAPSALRAQAKTYVVDAATGQGQFVPTSSPRALKAEELPDIVHH
                     YAAAARNAVHTAGFDGVELHGANGYLLDQFLKDGANQRRDDYGGAIANRVRLTLEVVR
                     AVVEAVGGGRVGIRLSPVTPSNDIYDSHPQPLFEHLAQQLAPLGLAYLHVIEGSTGGP
                     REIADRPFDYAAFKAAYRNAGGRGAWMVNNGYDRALALQAVASGHADMVAFGRAYIAN
                     PDLVLRLHRDEPLNPCDRTTFYGGGAKGYIDYPALG"
     gene            complement(2041..2649)
                     /gene="gstA"
                     /locus_tag="PK518_14630"
     CDS             complement(2041..2649)
                     /gene="gstA"
                     /locus_tag="PK518_14630"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HRM50193.1"
                     /translation="MKLYYSPGACSLSPHIVLHEAGLAYEPVLASTKTHQLADGTDFY
                     TINPLGYVPVLELDDGTRLREGPAIVQYLADQAPHKNLAPAHGTLGRYRLQEWLTFIG
                     TEIHKTFSPLFVPGTPEDYKPQVKQRLLARLQWVDSELAGKPYLMGEQFSVADAYLFT
                     VTNWGQYVGLDISQLKNLVAYRERIAARPAVQAAMKAEGLLK"
     gene            complement(2687..2980)
                     /locus_tag="PK518_14635"
     CDS             complement(2687..2980)
                     /locus_tag="PK518_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRM50194.1"
                     /translation="MDCRPGCGACCTAPSISSPIPGMPDGKPAGVPCVQLDEQLRCRL
                     FGQPERPEVCRSLHPHAQMCGDSRQEAEAYLLRLEQLTLPADPPTPARTAHKA"
     gene            complement(2990..3568)
                     /locus_tag="PK518_14640"
     CDS             complement(2990..3568)
                     /locus_tag="PK518_14640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM50195.1"
                     /translation="MKLLVFAGSTRQQSFNRRLARIATDLGREAGAEATLLELSELDI
                     PMYNADLEARGTPADVIRLKEVLDAHPAWIICSPEYNGSYTGLLKNTIDWASSPVAGH
                     PVWTDVTRPFAGKVVGVLSASNGALGGLRSQSHLAPMLVNLGCWLAPRNYALARAASA
                     FDEHGQLTDAAQREQVRGVVEQVLWAARRLTD"
     gene            3693..>4063
                     /locus_tag="PK518_14645"
     CDS             3693..>4063
                     /locus_tag="PK518_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM50196.1"
                     /translation="MLYPQEFDVIVVGGGHAGTEAALAAARMGCATLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMALATDESGIQFRILNSSKGPAVRATRAQADRI
                     LYKAAIRRMLENQPNLWLFQQA"
BASE COUNT          622 a         1303 c         1472 g          666 t
ORIGIN      
        1 cgggcgcgcc ccgggcctat cgtgcattcc atgcctggtg accgatcacg ggcattggca
       61 ggacgagaag gcggccctga cccagctcaa caccgagctg gacgcccgcg tggcggcgcg
      121 caccgcacgc atcgaacggc tggcgcagga tgcgcgccat gccgccatgc cgccatgccg
      181 ccgtgacgcg cgagcgcctg cgcctggcgc gcggcctgca tgacacgctg gcgcattcgc
      241 tgatggcgct gctgacgcag atccgcatga tgcgcaagct gggcccaagc tggagccgcg
      301 agcagctcga cgccgagctg gcggacaccg aaaccctggc cgccagcggc ctggtgcagg
      361 cgcgcgcggc catcgcccag atgcgcgacg ccggcgtgca cgacaacggc ctggggcccg
      421 aactgcaggc cctgctgcag cgcctggccg agcgcagcgg gctgcgggtc caggcgcata
      481 tcgatgccgc cgccgccgac ctggtggacg agcgcgccgc catggtgctg gccatggcgc
      541 gcgaggcgct gcgcaatgtg gagcgccatg ccggggcgca tcaggtgagc ttgtcgctgg
      601 cgcccctgga tgcgcccgat gccggtggcg acgcccccgc gccctggcgg ctggtgctgc
      661 gcgacgatgg ctgcggcttt gaccccggcg ctataccgga tggccatttc ggcctcgtcg
      721 gcctgcgcga acaggcccag cagctgggcg ccacgctgca tatagacagc gcgccggggc
      781 agggctgccg cgtgcagctg gactttgccg cctgactttc gtggcccgcg cgctcgtact
      841 gcggtgtggg agcggcggcc gctacccgag tgcggggtag tcgatataac ccttggcacc
      901 gccgccatag aaggtggtcc tgtcgcaggg gttcagtggc tcgtcacggt gcaggcgcag
      961 caccaggtcg gggttggcaa tataggcccg gccaaaggcc accatgtcgg catggccgct
     1021 ggccacggcc tgcagggcca gggcgcggtc gtagccgttg ttcaccatcc aggcgccgcg
     1081 cccgccggca ttgcggtagg cggccttgaa ggcggcgtag tcgaaggggc ggtcagcgat
     1141 ctcgcgcggg ccgccggtcg agccctcgat cacgtggaga taggccagac ccaggggcgc
     1201 cagctgttgc gccaggtgtt cgaacagtgg ttgcggatgg ctgtcataaa tgtcgttgga
     1261 tggagtaacc ggcgacaggc gtatgcccac gcggccgcca cccacggcct cgaccacggc
     1321 gcgcacgact tccagcgtca ggcgtacacg gttggcaatg gcgccgccgt agtcgtcgcg
     1381 gcgctggttg gccccgtcct tgaggaactg gtccagcaga tagccattgg cgccgtgcag
     1441 ctccacgccg tcaaacccgg cagtgtgcac ggcattgcgc gccgcggcgg catagtggtg
     1501 cacgatgtcg ggcagctctt cggccttgag cgcgcgcggg gacgatgtgg gcacgaactg
     1561 accctggccg gtggcggcat ccacgacata ggtcttggcc tgggcgcgca gggccgatgg
     1621 cgccacgggt gcggcgttgc ccggctgcag gctgcagtgc gacacgcgcc ccacatgcca
     1681 gagctgggtg acgattttgc cgccctgggc atgcacggcc tgggtgactt tcttccagcc
     1741 ctgcagctgc tcgctgtcgt acaggcctgg cacatcggaa tagccctggc cctgctggct
     1801 gatggccgtg gcctcggtga tcaacagacc ggcgctggcg cgctgggcgt agtaggtgac
     1861 cgccatgtcg cctggcaccg cccctggcga gcggttgcgc gtcagcggcg ccatggcgat
     1921 gcggttggcc agatgcaggt ctccagcccg gatgggatca aacaaggtgg tcatgcgtgt
     1981 ggcggcagag gggaaagaca aaggcccctg tttcacgtga aacaggggcc ggtggggcac
     2041 ttacttcagc aggccttcgg ccttcattgc cgcctgcacg gctgggcggg cggcaatgcg
     2101 ttcgcggtag gcgacgaggt ttttcagctg tgaaatgtcc aggccgacgt actgccccca
     2161 gttggttacg gtgaacagat aggcatcggc cacgctgaac tgctcgccca tcagataggg
     2221 tttgccggcg agctcgctat ccacccattg caggcgtgcc agcagccgtt gcttgacctg
     2281 gggcttgtag tcctcgggcg taccggggac gaacagcggg ctgaaggtct tgtggatctc
     2341 cgtgcctatg aaggtcagcc actcctgcag gcggtagcgg cccagcgtgc catgggctgg
     2401 tgccaggttt ttgtgcggcg cctggtcggc caggtattgc acgatggccg ggccttcgcg
     2461 caggcgggtg ccgtcgtcca gctccagcac cggcacatag cccagggggt tgatggtgta
     2521 gaaatccgtg ccgtcggcca gctggtgcgt cttggtgctg gccagcaccg gctcgtaggc
     2581 caggccggct tcgtgcagca cgatgtgggg cgagagcgag caggcgccgg gggagtagta
     2641 cagcttcatt cggtggttct cctgtggttg acgaaacctg tcgatcctat gccttgtgcg
     2701 ccgtccttgc aggggtaggc ggatcagcgg gcagggttaa ctgctccagc cgcagaagat
     2761 aggcctcggc ttcctggcgt gagtcgccgc acatctgtgc atgcgggtgc agcgaacggc
     2821 aaacctctgg gcgctcgggc tggccgaaga ggcggcagcg cagctgttcg tcgagctgta
     2881 cgcagggtac gcctgccggc ttgccgtccg gcatgcccgg aatcggcgac gagatggacg
     2941 gcgcggtgca gcaggcgccg cagccggggc ggcagtccat gaccaagact caatcggtga
     3001 gccgccgtgc cgcccacagc acctgctcca ccacgccgcg cacctgctcg cgctgggctg
     3061 cgtcggtgag ctggccatgc tcatcgaagg cgctggccgc gcgcgccagt gcgtagttgc
     3121 gcggcgccag ccagcagccc aggttgacca gcatgggtgc caggtgactt tgcgagcgca
     3181 ggccgcccag tgcgccgttg gaggcgctca gcacccccac caccttgccg gcgaaggggc
     3241 gcgtcacatc ggtccacacc gggtggccgg ccacggggct ggaggcccag tctatggtgt
     3301 tcttcagcag gccggtgtag ctgccgttgt attcgggcga gcagatgatc caggcagggt
     3361 gcgcgtccag aacttccttg agccggatca cgtcggcagg ggttccgcgt gcctccagat
     3421 cggcgttgta catggggatg tccagctccg acaactccag caaggtggcc tcggcgccgg
     3481 cctcgcggcc caggtcggtg gcgatgcgcg ccaggcggcg gttgaacgat tgttggcggg
     3541 tgctgcccgc gaagacgagt agtttcatgg ggtggattgg agcacagcca ccgtgccgga
     3601 ctgcggcgag ggtttcacgt gaaacatcgc aaccgggcag ggctgtggca aagatgggaa
     3661 aattgcgggt tttcccgtca ccgagccccc tcatgctgta cccccaggaa tttgatgtca
     3721 tcgtcgtcgg cggtggccac gccggcaccg aggccgcgct ggccgccgcg cgcatgggct
     3781 gcgccacgct gctgctcacg cacaacatcg agacactggg gcagatgagc tgcaacccca
     3841 gcattggcgg cattggcaag ggccatctgg tgaaagaggt ggacgcgctg ggcggcgcca
     3901 tggcgctggc gaccgacgag tcgggcatcc agttccgcat cttgaacagc agcaagggcc
     3961 cggcggtgcg cgccacgcgc gcgcaggccg accgcatttt gtacaaggcg gcgattcgcc
     4021 gcatgctgga gaaccagccg aatctgtggc tgttccagca ggc
//
LOCUS       DAOMTQ010000215         4054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_5972_length_4054_cov_27.965741, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000215 DAOMTQ010000000
VERSION     DAOMTQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5972_length_4054_cov_27.965741"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PK518_14650"
     CDS             <1..1358
                     /locus_tag="PK518_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM50197.1"
                     /translation="VGDDDQSIYGWRGATLDNLKKLPQDFPQLKVIKLEQNYRSTSAI
                     LRAANNVIGPNPKLFPKTLFSELGEGEPVRVVPCDNEEHEAERAVARIQSLRAAANPP
                     PAWKNFAILYRANHQAKPFEKALRKANIPYKVSGGTSFFDRAEIRDLCAWFRLWINND
                     DDPAFLRAITAPKRGVGHTTLGALGEFATQHKQSMFGALFNAMLPAVVPKRGLEGLME
                     FGRQINELEHRARHTHGAEDARAFLTDWLKDIGYEQHLYDGEDSEKVAAARWSNVLEF
                     CDWMAQRAGGQIDDTAATTVKPTKSLLEVAQTIALLSTISEREQDQDMVVLSTLHASK
                     GLEWPHVMLVGVTEGMLPFKLDDDDGRQQKVSDDIAARLQEERRLMYVGITRAQRSLA
                     VSWTKKRKKGREMVACQPSRFIAEMKLDAATTREDPREKLRQLRAEFAAKAQAAPNAT
                     P"
     gene            1467..1784
                     /locus_tag="PK518_14655"
     CDS             1467..1784
                     /locus_tag="PK518_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50198.1"
                     /translation="MEQPELIGRWQADMPGLAGPIVLELAPHPEWDGTVKGRILRPGF
                     SAIVVGDVHQGQLTMEESHDGRKVTGNWSGEVVEGSCAREIRGEWADEADRPFAFTLR
                     KLP"
     gene            1781..2932
                     /locus_tag="PK518_14660"
     CDS             1781..2932
                     /locus_tag="PK518_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HRM50199.1"
                     /translation="MTRAALATLIGAALPLMAQAQAPVAGDAGWRQCSALADGQARLA
                     CFDQWAGHQAWAAPAASASAQPAEDAATAPAAPTTPLAGRQPPAVADDTPGDGCRGTE
                     YTTLSRFWELEAGTDCGALRFRGYRPMSVSIVGSDSVNQQPTSASADHSASKPVDYRK
                     TEMRLQLSVRTKLAKGLLTQGNSQLRDSLWFGYSQQSYWQLFSPGISRPFRTTDHEPE
                     ILYVYPTDAQLPWGWRWRYSGIGLVHQSNGQSLPLSRSWNRVYLMTGLELDDRWRLNA
                     RIWKRLPESAANDDNPGISNYIGRAELGLGWNLDRNNTLVLTTRHSLSSTARGSARLE
                     WLYSLGTSWVGQSTNLRLYTQLFTGYGDSLIDYNRKRTVFSVGVSLVDF"
     gene            3146..3532
                     /locus_tag="PK518_14665"
     CDS             3146..3532
                     /locus_tag="PK518_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50200.1"
                     /translation="MELQYELKAGNYYLYDMRDAPSSVTGERRCKLKTDSVAIAFDQH
                     TGQVHQHGSPARIQSWAMHQRRRLRAAGALDQANGIVVVSGPLPVDELNKCLWISGYC
                     RRMFTRLASLPHGKLQGHNNQWKKAA"
     gene            complement(3596..>4054)
                     /locus_tag="PK518_14670"
     CDS             complement(3596..>4054)
                     /locus_tag="PK518_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM50201.1"
                     /translation="LALDAVRHGIALVRRGGLQATLVLGHVQEEATFMELATRGADMV
                     AAASVEAAQDLMASAVALVDAAGVPYETEIGLGAVAATLVDMVERCGCDLLFIGARGL
                     GGLRGAFLGSVSQSLVHISPVPVTVVKHAEPQELPDDDEDDEDDEPAAGE"
BASE COUNT          740 a         1478 c         1279 g          557 t
ORIGIN      
        1 ccgtgggcga cgacgaccag agcatctacg gctggcgcgg cgccacgctg gacaatttga
       61 agaagctgcc gcaggacttt ccgcagctca aggtcatcaa gctggagcag aactaccgct
      121 ccacctcggc catcctgcgc gcggccaaca acgtcatcgg cccgaatccc aagctgttcc
      181 ccaagacgct tttcagcgaa ctgggcgagg gcgagccggt acgcgtggtg ccctgcgaca
      241 acgaggagca cgaggccgag cgcgccgtgg cccgcatcca atcgctgcgc gccgccgcca
      301 acccgccgcc ggcctggaag aacttcgcca tcctgtaccg cgccaaccac caggccaagc
      361 cgttcgagaa ggcgctgcgc aaggccaaca tcccctacaa ggtctcgggc ggcacgtcgt
      421 tctttgaccg cgccgagatc cgcgacctgt gcgcatggtt ccgcctgtgg atcaacaacg
      481 acgacgaccc ggcctttctg cgcgccatca ccgcgcctaa gcgcggcgtg ggccacacca
      541 cgctgggcgc cctgggcgaa ttcgccacgc agcacaagca aagcatgttc ggcgcgctgt
      601 tcaacgccat gctgccggcc gtggtgccga aacgcgggct agaggggctg atggagttcg
      661 gccgccagat caacgagctg gagcaccgcg cgcgccacac gcatggggcc gaggatgcgc
      721 gcgccttcct caccgactgg ctgaaggaca taggctacga gcagcatctg tacgacggcg
      781 aggacagcga gaaggtggcc gccgcgcgct ggagcaatgt gctggagttc tgcgactgga
      841 tggcccagcg cgcgggcggg cagatcgacg acaccgcggc caccaccgta aagcccacca
      901 agagcctgct ggaggtggcg cagaccattg ccctgctgtc caccatctcc gagcgcgaac
      961 aggatcagga catggtggtg ctctccaccc tgcacgccag caagggcctg gagtggccgc
     1021 atgtgatgct ggtcggcgtg accgagggca tgctgccctt caagctcgac gatgacgacg
     1081 gccggcagca aaaggtcagc gacgacatcg ccgcgcgcct gcaggaagag cgccgcctga
     1141 tgtacgtggg catcacccgc gcccagcgca gcctggccgt gagctggacc aagaagcgca
     1201 agaagggccg cgagatggtg gcctgccagc ccagccgctt catcgccgag atgaagctgg
     1261 acgccgccac cacccgcgaa gacccgcgcg agaagctgcg ccagctgcgc gccgaattcg
     1321 ccgccaaggc ccaggccgcc cccaacgcca ctccctgaat catgcgagta actatatttt
     1381 taatagctgt cagcgcttgt ctgataagcg cttgtgccca aaaccattca aaaaccacac
     1441 gccccgcctg cccccaggcc gcgcagatgg agcagcccga gctcatcggc cgctggcagg
     1501 ccgacatgcc gggcctggcc ggccccatcg tgttggagct ggcgccccac cccgaatggg
     1561 acggcaccgt caagggccgc atcctgcgcc ccgggtttag cgccatcgtg gtgggcgacg
     1621 tacaccaggg ccagctgacc atggaagagt cgcacgacgg caggaaggtc accggcaact
     1681 ggagcggcga ggtggtcgaa ggcagctgcg cgcgtgagat ccgcggcgaa tgggcggacg
     1741 aggccgaccg gccgttcgcc ttcacattgc gcaagctgcc atgacgcgcg ccgctcttgc
     1801 caccctgata ggcgccgccc tgccgctgat ggcccaggcg caagcccccg tggccggcga
     1861 tgccggctgg cgccaatgtt ccgccctggc cgatggccag gcgcgcctgg cctgctttga
     1921 ccagtgggcg ggccaccagg cctgggccgc gcccgcggca tcggccagcg cccagccggc
     1981 ggaagacgcg gcgaccgcgc cagccgcgcc aaccacgccg ctggcgggcc gccagccccc
     2041 cgccgtggcc gatgacaccc ctggcgacgg ctgccgtggc accgaataca ccaccctgtc
     2101 gcgcttctgg gagctggagg ccggcaccga ctgcggcgcg ctgcgctttc gcggctaccg
     2161 gcccatgagc gtgtccatcg tcggctcgga cagcgtgaac cagcagccga catccgccag
     2221 cgcggaccac agcgccagca agcccgtgga ctaccgcaaa accgagatgc gcctgcagct
     2281 gtcggtgcgc accaagctcg ccaaggggct gctcacgcag ggcaactcac agctgcgcga
     2341 ctcgctgtgg tttggctaca gccagcagtc gtactggcag ctcttcagcc ccggcatctc
     2401 gcggccgttt cgcaccaccg accacgagcc cgagatcctc tacgtctacc ccaccgatgc
     2461 gcagctgccc tggggctggc gctggcgcta cagcggcata ggcctggtgc accagtccaa
     2521 cggccagagc ctgccgctgt cgcgcagctg gaaccgcgtg tacctgatga ccggcctgga
     2581 gctggatgac cgctggcggc tcaacgcccg catctggaag cgcctgcccg aaagcgccgc
     2641 gaacgacgac aaccccggca tcagcaacta catcggccgc gccgagctgg gcctgggctg
     2701 gaacctggac cggaacaaca ccctggtgct gaccacgcgc cactcgctca gctccaccgc
     2761 gcgcggctcg gcgcggctgg aatggctgta cagcctgggc accagctggg tcggccaaag
     2821 caccaacctg cgcctgtaca cgcagctatt caccggctac ggcgacagcc tgatcgacta
     2881 caaccgcaaa cgcaccgtgt tcagcgtggg cgtgagcctg gtggacttct gagcaccaca
     2941 cccggttacc gtttgccccg cgccacgcct tgaagcgccc ctgggccggg cagacaatcg
     3001 caggcggcca cccctgctat cgaccttgag gtggtcataa ggccatgcct atcgggctgg
     3061 cgccgataag gcaccggttt cgcacggttt tcaggccttt gcccgcgggc cttccccgca
     3121 acaatgccca ccggaggagg agaccatgga actgcaatac gagctcaagg ccggcaacta
     3181 ctacctgtac gacatgcgcg atgcgcccag cagcgtcacg ggcgagcgcc gctgcaagct
     3241 caagaccgac agcgtggcca tcgccttcga ccagcacacc ggccaggtgc accagcatgg
     3301 cagcccggcg cgcatccagt cctgggccat gcaccagcgc cggcgcctgc gcgcggccgg
     3361 cgcgctggac caggccaacg gcatcgtcgt cgtctccggt ccgctgccgg tggacgagct
     3421 gaacaaatgc ctgtggatca gcggctactg ccggcgcatg ttcacgcgcc tggccagcct
     3481 gccgcatggc aagctgcaag gccacaataa tcaatggaaa aaagccgcct aaggcttatc
     3541 aacaaagcgt caacagctca ctatttaata gcgaacgagc cctctggccc gttcttcatt
     3601 cgcctgctgc aggctcgtcg tcctcatcgt cctcgtcatc gtccggcagc tcctgcggct
     3661 cggcatgctt gaccacggtg accggcaccg ggctgatgtg caccaggctt tgcgacaccg
     3721 atcccaggaa ggccccgcgc aggccgccca ggccgcgcgc gcctatgaac agcaggtcgc
     3781 agccgcagcg ctccaccatg tccaccagcg tggcggccac cgcacccagg ccgatctcgg
     3841 tctcataggg cacaccggcg gcgtccacca gggccacggc cgaggccatc agatcctgcg
     3901 ccgcctccac gctggcggcg gccaccatgt cggcgccgcg cgtggccagc tccatgaacg
     3961 tagcctcctc ctgcacatgg cccagcacca gcgtggcctg caggccgccg cggcgcacga
     4021 gggcgatgcc atggcgcacg gcgtccagcg ccag
//
LOCUS       DAOMTQ010000216         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6083_length_4003_cov_26.835360, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000216 DAOMTQ010000000
VERSION     DAOMTQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6083_length_4003_cov_26.835360"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PK518_14675"
     CDS             <1..410
                     /locus_tag="PK518_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRM50202.1"
                     /translation="IADPTPAPAAPVGVVEPQPAAKPVEAPVAAAPAPAAPPAPAIVQ
                     PSSDASHLNNPKPVYPAVSKRLGEQGKIVLRVLIGADGIPQKVEIKQSSGFERLDRQA
                     VDTVTRWRFVPGTRNGVPEAMWYLQPINFVLQQ"
     gene            426..1145
                     /locus_tag="PK518_14680"
     CDS             426..1145
                     /locus_tag="PK518_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRM50203.1"
                     /translation="MESQFGIANVWIQGDLVTRAVALLLLGMSLASWIVILIKALDII
                     KYQKHARLARDFWHSEDFAAGLTKLGADPSNPFRHLALEGREATAHHRNTQAHLHDSL
                     DVSDWVTRCLRNCIDEFTARLQSGLAILASVGSTAPFIGLFGTVWGIYHALVAIGASG
                     QSTIDKVAGPIGEALIMTALGLAVAIPAVLGYNALVRGNKTILGSLNSFAHDLHAYFV
                     TGARVTVQGEGAARVLPIKKG"
     gene            1151..1561
                     /locus_tag="PK518_14685"
     CDS             1151..1561
                     /locus_tag="PK518_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRM50204.1"
                     /translation="MAFGTQDDADEVMNEINMTPLVDVMLVLLIIFIITVPVMKHAVP
                     VDLPRASNEREVIKPETIRLSVTADGKYHWNETTISDEELEPRLQAEAQKDPQPDLHI
                     RGDKDVRYERVAQAMSAAQRAGVRKIGFVTDPAQ"
     gene            1677..1898
                     /locus_tag="PK518_14690"
     CDS             1677..1898
                     /locus_tag="PK518_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HRM50205.1"
                     /translation="MQLSLTAHTTAPRSRLFAHGDVGRSEASEHLPAVVTALDSNNLL
                     RGRKAVTIVHNGASYRLQATKLGKLILTK"
     gene            complement(2047..2454)
                     /locus_tag="PK518_14695"
     CDS             complement(2047..2454)
                     /locus_tag="PK518_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRM50206.1"
                     /translation="MKTKPELELADLKLIAAAAEAEALKNHWAVTIAIVDDGGHLLWL
                     QRLDGAPAISAHIAPAKAHTAALGRRESKGYEDVINGGRTSFLSAPTVQGMLEGGVPI
                     MKDGQCLGAVGVSGVKSSEDAQIARAGIAAIAL"
     gene            2714..3406
                     /locus_tag="PK518_14700"
     CDS             2714..3406
                     /locus_tag="PK518_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRM50207.1"
                     /translation="MNTQVTQIRTGGYGISQEERNRVLRNTYWLLALSLLPTVLGAWL
                     GVATGITESLRGGLGLIVFLGGAFGFMFAIEKTKNSAAGVAVLLGFTFFMGLMLSRMI
                     GMVLGFKNGTDLIMTAFAGTAGVFFVMASLATVIKRDLSGMGKWLFVGALVLMVGAIV
                     NVFVGSSAGMMAISVAAIGIFSAYMLYDLKQILDGGETNYISATLALYLDLFNVFQSL
                     LALLGIMGGERD"
     gene            3507..3887
                     /locus_tag="PK518_14705"
     CDS             3507..3887
                     /locus_tag="PK518_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50208.1"
                     /translation="MPPLNPTHRLARIPLLALALLPLAGCEIPSLPQDPRVVQREAEA
                     KAVGGACRHALRGLEDCFTLNPKAAKAQVFAGWKEMDQYMRENKLEGAPSVLTQLEQK
                     PAAAPARSPEGDEEDPPRSSRKRG"
BASE COUNT          760 a         1363 c         1221 g          659 t
ORIGIN      
        1 ccattgccga ccccacgccg gcacccgccg cccccgtggg cgtggtcgag ccacaacctg
       61 cggcaaagcc cgtcgaggcg cccgtggccg ccgccccggc gcccgcggcc ccgcccgctc
      121 cggccatcgt tcagcccagc agcgacgcca gccacctgaa caaccccaag ccggtctacc
      181 ccgccgtgag caagcgcctg ggcgaacagg gcaagatcgt gctgcgcgtg ctgatcggtg
      241 ccgacggcat tccgcaaaag gtcgagatca agcagtccag cggcttcgag cgcctggacc
      301 gccaggccgt ggacacggtc acgcgctggc gcttcgtgcc cggcacgcgc aacggcgtgc
      361 ccgaggccat gtggtacctg caacccatca acttcgttct ccaacaatag cccccgggag
      421 ttttcatgga atcgcaattt ggcatcgcca acgtctggat acagggggat ttggtcaccc
      481 gcgccgtcgc cctgctgctg ctgggcatgt cgctggcctc gtggatcgtc atcctcatta
      541 aggcgctgga catcatcaaa taccagaagc acgcccgcct ggcgcgcgac ttctggcaca
      601 gcgaggactt tgccgccggc ctgaccaagc tgggcgccga cccgagcaac ccgtttcgcc
      661 acctggcact ggaggggcgc gaggccacgg cacaccaccg caacacccag gcgcatctgc
      721 acgacagcct ggacgtgagc gactgggtca cgcgctgcct gcgcaactgc atcgacgagt
      781 tcaccgcccg tctgcaatcg ggcctggcca tcctggcgtc ggtgggctcc accgcaccct
      841 tcattggcct gttcggcacc gtctggggca tctaccacgc gctggtggcc attggcgcct
      901 cgggccagtc caccattgac aaggtagccg gcccgattgg cgaggcgctg atcatgaccg
      961 ccctgggcct ggccgtggcc atcccggcgg tactgggcta caacgccctg gtgcgcggca
     1021 acaagaccat tctgggcagc ctgaacagct ttgcgcacga cctgcacgcc tatttcgtga
     1081 ctggcgcacg cgtgaccgtg caaggcgaag gcgcggcccg cgtgctgccc atcaagaaag
     1141 gctgacgcgc atggccttcg gtacccagga cgacgccgat gaggtgatga acgagatcaa
     1201 catgacgccg ctggtggacg tcatgctggt gctgctcatc atcttcatca tcaccgtgcc
     1261 cgtcatgaag cacgccgtgc cggtcgatct gccacgcgcc agcaacgagc gcgaggtgat
     1321 caagcccgag accatacgcc tgtcggtgac ggcggacggc aagtaccact ggaacgaaac
     1381 caccatcagc gacgaggagc tggagccacg cctgcaggcc gaggcgcaga aagacccaca
     1441 gccggacctg cacatccgcg gcgacaagga cgtgcgctat gaacgcgtgg cccaggccat
     1501 gtcggccgcc cagcgcgccg gtgtgcgcaa gatcggcttt gtgaccgacc cggcgcaata
     1561 agcgcggcag caggcatccg tttgaagcaa gaagatacga aattcggtac aaacaatgct
     1621 tgacatgtaa ttgcgaatca ttatcattca ataatcgcaa cacaacgaga cagtccatgc
     1681 agctcagcct gaccgcacac accacagcac cgcgctcacg cctctttgcc catggtgacg
     1741 tgggtcgatc cgaagcctcg gaacatctgc cggcagttgt cacagcactg gacagcaaca
     1801 acctgctgcg cggccgcaag gcggtgacca tagtgcacaa cggcgccagc taccgtctgc
     1861 aggccaccaa gctgggcaag ctgatactga ccaaatagtt ttcatcgtgg ggcgactcgg
     1921 ggacttcggt tcaagggtcg tttttggcga gccaaggggc atgcccgcat agccaagcct
     1981 tttttcacac gctgagacag acaaaaaagc cggcatccag ccggcttttt catggggcga
     2041 agttgtttac agagcgatgg ccgcgatgcc ggcgcgtgca atctgcgcgt cctcgctgga
     2101 cttgacgccg ctcacgccca cggcgcccag gcactgaccg tccttcatga tgggcacgcc
     2161 gccctcgagc atgccctgca ccgtgggcgc gctcagaaac gaggtacggc caccattgat
     2221 cacatcctcg tagcccttgc tctcgcggcg gcccagggcg gccgtgtgcg ccttggccgg
     2281 cgcgatgtgg gcggaaatgg cgggcgcacc atccaggcgc tgcagccaca gcaggtggcc
     2341 gccatcgtcc acgatggcaa tggtcacggc ccagtggttc ttcagcgcct cggcctctgc
     2401 ggcagcggcg atgagcttca ggtcggcgag ttcaagctcg ggcttggttt tcatgagaaa
     2461 aatcctgtag agatcaatga gggacgcgcg ccagcccacg gcaggcgcgc agcgtgtggg
     2521 gagcatagcg ccgcaagccc agcacaatga accaatgcaa ggcaaatgaa tccgaccttt
     2581 tagtaccaga ctgcccggcg cagaaaaggc gccgtgccgg gtatctggtg ggatgacacc
     2641 cgcatgccgc ggcgcaccac tagaatgcgc cgtgccgcca ccatcgcagg ccattgcagg
     2701 agaacaccaa gctatgaaca cacaagtcac ccagatccgc accggcggct atggcatatc
     2761 gcaagaagag cgcaaccgcg tgctgcgcaa cacctactgg ctgctggcgc tgagcctgct
     2821 gcccaccgtg ctcggcgcat ggctgggcgt ggccacgggt atcaccgagt cgctgcgcgg
     2881 cgggctgggg ctgatcgtct tccttggcgg cgccttcggc ttcatgttcg ccatcgagaa
     2941 gaccaagaac tcggccgccg gcgtggccgt gctgctgggc ttcaccttct tcatgggcct
     3001 gatgctctcg cgcatgatcg gcatggtgct gggcttcaag aacggcaccg acctgatcat
     3061 gaccgccttt gccggcacgg ccggcgtgtt cttcgtcatg gccagcctgg ccacggtgat
     3121 caagcgcgac ctctcgggca tgggcaagtg gctgttcgtg ggcgcgctgg tgctcatggt
     3181 gggcgccatc gtcaacgtgt tcgtgggctc ctcggccggc atgatggcca tctcggtggc
     3241 ggccatcggc atcttcagtg cctacatgct gtacgacctc aagcagatcc tggacggcgg
     3301 cgagaccaac tacatcagcg ccacgctggc cctgtacctg gacttgttca acgtgttcca
     3361 gagcctgctg gccctgctgg gcatcatggg cggcgagcgc gactgaccga cagcgctctt
     3421 ccagccaaga aaggccccgg ctgcgcaccg gggctttttt cttcgcgccc tcaacttatg
     3481 gcccaggctg ccgatatcag gcatacatgc caccccttaa ccccacccac cgtcttgccc
     3541 gcatccccct gctcgccctg gcgctgctgc cactggcggg ctgcgagatc cccagcctgc
     3601 cccaggatcc gcgcgtggtt cagcgcgagg ccgaggccaa ggccgttggc ggcgcctgcc
     3661 gccatgcgct gcgcgggctg gaggactgct tcaccctgaa ccccaaggcg gccaaggccc
     3721 aggtgtttgc gggctggaag gagatggacc agtacatgcg cgagaacaag ctcgagggcg
     3781 caccctcggt gctcacgcag ctggagcaaa agccggccgc cgcccctgcc cgcagccccg
     3841 agggtgatga agaagaccca ccgcgcagct cgcgcaagcg tggttgatgc tattgtaaaa
     3901 atagctatcc gcgcttacct gtaaagcgcc aaagccaaaa aatgcctgac taggcacata
     3961 cctcagactg ggccgcgaac cctcaccccc accctctccc aga
//
LOCUS       DAOMTQ010000217         4002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6085_length_4002_cov_24.834558, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000217 DAOMTQ010000000
VERSION     DAOMTQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_4002_cov_24.834558"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PK518_14710"
     CDS             <1..1333
                     /locus_tag="PK518_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM50209.1"
                     /translation="GGWPAVAPLAAMSPALEAALKANAANAQPLPQAHTPAPLATWLL
                     ALAGAVLGGLILNLMPCVFPVLAIKVVGFARHAQDARAHRVSGLAYTAGVLLSFLALG
                     ALMLALRAAGEAVGWGFQLQSPAVVAALAALFTLIALNLAGMFEFRAMLPQSLAGLQA
                     RHPVLDAFLTGVLAVAVASPCTAPFMGASLGLTAQLPAAQALSIFAALGLGLALPYLL
                     ASWLPAFARALPRPGAWMETLRRFLAFPMFATVVWLVWVLGQQSGIHGAAALLALLVL
                     LAMAVWALTLRGLSRAVLAPLSIAACAWFTWAWGPKIIELTPHASETATAAPVGASAW
                     QAWRPGLADELLAQGRPVFVDYTAAWCVTCQYNKSTTLAKTEVLGDFAAKQVALLRAD
                     WTRRDPAITAALAALGRSGVPVYVLHAPGREPVVFSEILNADALRAAVHGL"
     gene            1395..2339
                     /locus_tag="PK518_14715"
     CDS             1395..2339
                     /locus_tag="PK518_14715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM50210.1"
                     /translation="MSGCLPPSASSLPASCDALPAVVLELLQASEQLIGIFDAQDILR
                     FANPPFQQAYHARPDGRRTWGDIMRANYLQQRGTPVQTQDFDRWLASARSRRGKQAFR
                     AFETELHDGRWIWMAETTLAGGWMLCVASDISALSQDGRSLRQTRDQALRTAQTDDLT
                     GISNRAHMLQQADALIAQGQPLVLVLLDLDHFKGINDRFGHPAGDEVLRDFARQLQAS
                     TRRDDGCGRIGGEEFMMLLPGTDLPQARAVVQRLLEHVRAAHPLAEAPAQGYTTSAGL
                     ALHRAGECTGALYRRADQALYRAKQAGRDRLVCDGAPA"
     gene            2392..2616
                     /locus_tag="PK518_14720"
     CDS             2392..2616
                     /locus_tag="PK518_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRM50211.1"
                     /translation="MHQLKLTQIGNSVGVILPKEALARLKLQKGETVFLTESPEGFIL
                     TPYDPALEEEITAGRDFMREYRGTFHELAK"
     gene            2613..2999
                     /locus_tag="PK518_14725"
     CDS             2613..2999
                     /locus_tag="PK518_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HRM50212.1"
                     /translation="MSWRWISKQALLLLHAESLAEHGGGQGLRDEGLLDSALTRPQNL
                     AAYGNPDHAALAASYGLGLAKNHAFVDGNKRAALLATGLFLYLNGWRLTASQADTTLA
                     MLSLAAGELSEDAFAAWLRRNSAPRG"
     gene            3056..3394
                     /locus_tag="PK518_14730"
     CDS             3056..3394
                     /locus_tag="PK518_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HRM50213.1"
                     /translation="MKAVFVELPAFARHRADYLDDDGFRGLQAAMMANPQAGDVIEGT
                     GGLRKLRHADPRRGKGKRGGLRVIYYWWDGGPQFWLFTLYDKDEMADLTPAECKALKH
                     LLKTELEARQ"
     gene            3391..3705
                     /locus_tag="PK518_14735"
     CDS             3391..3705
                     /locus_tag="PK518_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM50214.1"
                     /translation="MNKRNLFAELTEGFDALAQARAGKKTLRTHEVTTPPAPTITAAQ
                     LLALRERLHLSRPVFAGYLRTNPRTLENWEQGRAQPNAQAALLIRLVEKYPDTVQRLA
                     TV"
     gene            3738..>4002
                     /locus_tag="PK518_14740"
     CDS             3738..>4002
                     /locus_tag="PK518_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50215.1"
                     /translation="MGDSHHANSAATPADADNNHAAAAFIARWQGSSASELATAQSFV
                     IELCQLLGVDRPHPTPAQDYMFERPITFWHGDGASSSGRIDCYK"
BASE COUNT          636 a         1497 c         1275 g          594 t
ORIGIN      
        1 cggcggctgg cccgccgtgg cgccactggc cgccatgtcg ccggcgctcg aggccgcgct
       61 caaggccaac gccgccaacg cgcagcccct gccccaggca cacaccccgg cgccgctggc
      121 cacctggctg ctggcgctgg ccggcgcggt gctcggcggc ctgatcctca acctcatgcc
      181 ctgcgtgttc ccggtgctgg ccatcaaggt ggtgggcttt gcgcgccacg cgcaggacgc
      241 gcgcgcgcac cgcgtcagcg gcctggccta taccgccggg gtgctgctgt cctttctggc
      301 gctgggcgcg ttgatgctgg ccctgcgcgc cgccggcgag gccgtgggct ggggctttca
      361 gctgcagtcg ccggccgtgg tggcggcact ggccgcgctg ttcacgctga tcgcgctgaa
      421 cctggccggc atgttcgagt tccgcgccat gctgccgcag tcgctcgccg gcctgcaggc
      481 gcgccacccg gtgctggacg ccttcctgac cggcgtgctc gccgtcgccg tggcgtcgcc
      541 ctgcacggcg ccgttcatgg gcgcgtcgct cggcctcacg gcccagctgc cggccgcgca
      601 ggcgctgtcc atcttcgccg ccctgggcct gggcctggcc ctgccctatc tgctggccag
      661 ctggctgccg gcctttgccc gcgccctgcc ccggcccggc gcctggatgg agacactgcg
      721 ccgcttcctg gcctttccca tgttcgccac cgtggtctgg ctggtctggg tgctgggcca
      781 gcaaagcggt atccacggcg cggccgcgct gctggcgctg ctggtgctgc tcgccatggc
      841 cgtctgggcg ctgacgttgc gcggcctcag ccgggccgtg cttgctccac tatccatagc
      901 tgcctgcgct tggttcacat gggcctgggg cccaaaaatc attgaactga cgccccatgc
      961 cagcgaaacc gcaaccgcgg ccccggtggg cgcatccgcc tggcaggcct ggcgcccggg
     1021 cctggccgat gaactgctgg cccagggccg cccggtgttc gtggactaca ccgccgcctg
     1081 gtgcgtgacc tgccagtaca acaagagcac caccctggcc aaaaccgaag tgctgggcga
     1141 ctttgccgcc aagcaggtgg ccctgctgcg cgccgactgg acgcggcgcg acccggccat
     1201 caccgccgcc ctcgccgcgc tgggccgcag cggcgtgccg gtctacgtgc tgcacgcgcc
     1261 gggacgcgag ccggtggtgt tctccgagat cctgaacgcc gacgcgctgc gcgcggccgt
     1321 gcatgggctg tagacggcac cccgggcagc cgatatcgtt atgatgcatg taataaattg
     1381 atacatattc atgcatgtct ggctgcctcc ctccctccgc aagctcacta ccggcatcgt
     1441 gcgacgccct gcccgccgtg gtgctggagc tgctgcaggc cagcgaacag ctgataggca
     1501 tctttgacgc gcaggacatc ctgcgctttg ctaatccgcc cttccagcag gcctaccatg
     1561 cgcgccccga tggccgccgc acctggggcg acatcatgcg cgccaactac ctgcagcagc
     1621 gcggcacgcc ggtgcagacg caggacttcg accgctggct ggcctcggcg cgctcgcgcc
     1681 ggggcaagca ggcctttcgc gccttcgaga ccgagctgca cgacggccgc tggatctgga
     1741 tggccgaaac cacgctggcg ggcggctgga tgctgtgcgt ggccagcgac atcagcgccc
     1801 tgagtcagga cggccgcagc ctgcgccaga cacgcgacca ggccctgcgc accgcgcaga
     1861 ccgacgacct taccggcatc agcaaccgcg cccatatgct gcagcaggcc gacgccctca
     1921 tcgcccaggg ccagcccctg gtgctggtgc tgctggatct ggaccatttc aagggcatca
     1981 acgaccgctt tggccacccc gccggcgacg aggtgctgcg cgactttgcg cgccagctgc
     2041 aggccagcac ccggcgcgac gacggctgcg ggcgcatcgg cggcgaggaa ttcatgatgc
     2101 tgctgcccgg caccgacctg ccccaggccc gcgccgtggt gcagcgcctg ctggagcatg
     2161 tacgcgccgc ccacccgctg gccgaggcac cggcgcaggg ctacaccacc agcgccggcc
     2221 tggccctgca ccgcgccggc gaatgcaccg gcgcgctgta ccgccgcgcc gaccaggccc
     2281 tgtaccgcgc caagcaggcc gggcgcgacc ggctggtgtg cgacggcgct cctgcatagc
     2341 gcgcgaggcg gtgctagtat tacaaacgta ataacgtttg tgaggcaccc catgcaccag
     2401 ctcaaactca cccagatcgg caattccgtc ggcgtcatcc tgcccaagga ggccctggcc
     2461 cgcctgaagc tgcaaaaggg cgaaacggtg tttctgaccg aatccccaga aggcttcatc
     2521 ctcacgccct acgacccggc gctggaagaa gaaatcaccg ccggccgcga cttcatgcgc
     2581 gaataccgcg gcaccttcca cgagctggcc aaatgagctg gcgctggatc agcaagcagg
     2641 ccctgctgct gctgcacgcc gaaagcctgg ccgagcacgg cggcggccag ggcctgcggg
     2701 acgaaggcct gctcgactcc gccctaacca ggccgcaaaa cctggccgcc tatggcaatc
     2761 ccgaccacgc cgccctggcc gccagctacg gcctggggct ggccaagaac cacgctttcg
     2821 tcgatggcaa caagcgcgcc gccctgctgg ccacgggcct gttcctgtac ctcaacggct
     2881 ggcgcctcac cgcgagccag gccgacacca cactggccat gctcagcctc gccgctggcg
     2941 agctgagcga agacgccttc gccgcctggc tgcgccgcaa cagcgcaccg cgcgggtaaa
     3001 cctcggtcag tacagggttg gctaacatac gcaatttgcg tatgatttga cccgcatgaa
     3061 agccgtcttc gtcgaactcc ctgccttcgc ccgccaccgg gcggactacc tggatgacga
     3121 cggatttcgc ggcctgcagg cggccatgat ggccaacccg caagcgggcg acgtgattga
     3181 aggaacgggt ggcctgcgca agctgcgcca tgccgaccca cgccgtggca aaggcaagcg
     3241 tgggggcctg cgggtgattt actactggtg ggatggcgga ccgcagttct ggctgttcac
     3301 cctgtacgac aaggacgaaa tggccgactt gacacccgcc gagtgcaagg ccctcaagca
     3361 tctgctgaaa accgaactgg aggcacgcca atgaacaagc gcaacctgtt tgccgaactg
     3421 accgagggat tcgacgccct ggcccaggcc cgcgccggca agaaaaccct gcgcacgcac
     3481 gaagtaacga caccgcccgc gcccaccatc acggcagcgc aattgcttgc cctgcgcgag
     3541 cgcctgcacc tctcgcgccc ggtgtttgcc ggctacctgc gcaccaaccc ccgcaccctg
     3601 gaaaactggg agcaagggcg cgcccagccc aacgcccagg ccgccctgct gattcgcctg
     3661 gtcgaaaaat accccgacac ggtgcagcgt ctggccacgg tctaatcacc cctaagcgac
     3721 ggctatcatc cccgcgcatg ggagatagcc accacgccaa cagcgcggcc acgccagccg
     3781 acgccgacaa caaccacgca gccgccgcct tcatcgcccg ctggcagggc agcagcgcca
     3841 gcgagctggc cacggcgcaa agcttcgtca tcgagctatg ccagctgctc ggggtggaca
     3901 ggccccaccc cacgcccgcg caggactaca tgttcgagcg ccccatcacc ttctggcatg
     3961 gcgacggcgc cagcagcagc gggcgcatcg actgctacaa gc
//
LOCUS       DAOMTQ010000218         3981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6127_length_3981_cov_25.004330, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000218 DAOMTQ010000000
VERSION     DAOMTQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6127_length_3981_cov_25.004330"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK518_14745"
     CDS             <1..354
                     /locus_tag="PK518_14745"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203270.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRM50216.1"
                     /translation="VSYAARGVPPEIMGIGPIEAIPAALRYAGLQSSDIGWYELNEAF
                     AAQSLAVMNTLGLDPAKVNPMGGAIALGHPLGATGAIRAATVVHALRRHKLKYGMVTM
                     CVGTGQGAAGIFESL"
     gene            412..1239
                     /locus_tag="PK518_14750"
     CDS             412..1239
                     /locus_tag="PK518_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRM50217.1"
                     /translation="MTHATHPFDDALALDPGAPDHFSGRTTPGYWNMVGPYGGITAAT
                     LLQAVLRHPLLLGEPLSLTVNYAAAVAEGPFTVQATPVRTNRSTQHWMVTVQQANAGG
                     EQMVTTTATVVTAARRETWGVSDQPMPTVPAPAQCQRTPPMFRVEWLNRYEMRPVTGE
                     LPTQWDDSGHTSLTQLWLRDAPERALDFAALAAMCDVFYPRVWLRRARHVPAGTVSIT
                     VYFHAGSALLAQTGDGYLLAQARAQEFRNGFFDQSAQLWNQAGSLLATSHQIVYYKE"
     gene            1245..2036
                     /locus_tag="PK518_14755"
     CDS             1245..2036
                     /locus_tag="PK518_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRM50218.1"
                     /translation="MSSTQDMLVHTEAGVTTLTFNRADKKNSITEAMYAAMADALAQA
                     AQDAAVRCVVFQGDMAIFSAGNDIADFLQQSSKGAAPELAGERPVWRFLRLLAGFPKP
                     LVAAVCGPAVGIGTTLLLHCDLVYAGDNAAFSLPFVNLGLCPEAASSLLLPQMLGYHR
                     AAEALLLGEPFMAEAALEVGLVNRVVPPAECNALAQAQARKLAAKPLSALIETKRLMK
                     AGQASEVQARMQEEGASFARLMAGPAAKEAFTAFMQKRKPDFSSC"
     gene            2234..2818
                     /locus_tag="PK518_14760"
     CDS             2234..2818
                     /locus_tag="PK518_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRM50219.1"
                     /translation="MAVQNPFFGKRDNDAFQARQPSPVPGGSNVTGQTSTSTTNPSTA
                     SSMNTSKPAASSTTESGGSKLTVGPNIKLKGVEITDCDTLVVEGTVEATMDSRVIQIT
                     EQGAFRGTAEIDIAEIHGEFDGSLTVREKLVIFSTGKVTGKIRYGKVVIEEGGQLSGE
                     ISVGVGGARAPAASTSAVTSSTSSAASSSTLAAA"
     gene            complement(2845..2991)
                     /locus_tag="PK518_14765"
     CDS             complement(2845..2991)
                     /locus_tag="PK518_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50220.1"
                     /translation="MRQPRGWRTFERDDEPLRYWFSVALYLVCAALGLGYGGVAVYAL
                     LLRT"
     gene            complement(3070..3492)
                     /locus_tag="PK518_14770"
     CDS             complement(3070..3492)
                     /locus_tag="PK518_14770"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRM50221.1"
                     /translation="MSQDRLSAATQLPTDKELVLKVIPMPADTNGNGDIFGGWVMAQV
                     DLAGSVLPARLIQGRMATVAVNEFIFKQPVRVGDLLSFFSSVTRLGNTSITVQVEVYA
                     EHIRAQGQHVKVTEARLTYVAIDDQGRPRPIAPAQLPA"
     gene            3536..>3981
                     /locus_tag="PK518_14775"
     CDS             3536..>3981
                     /locus_tag="PK518_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRM50222.1"
                     /translation="MRRHGDSSPPMSAAAEPKTRSDAATLARLLPYLWEYKWRVAAAI
                     ALMLGAKMANVGVPLLLKQLVDAMTIAPGAPAALLVVPVGLLLAYGALRFTNSLFNEL
                     RELVFAKATHGAARAIALRTFEHLHALSLRFHLERQTGGMTRDIERG"
BASE COUNT          647 a         1402 c         1307 g          625 t
ORIGIN      
        1 gtcagctacg ccgcgcgtgg cgtgccgccc gagatcatgg gcatcggccc catcgaggcc
       61 attcccgccg cgctgcgcta cgccggcctg cagtcctcgg acattggctg gtacgagctc
      121 aatgaggcct tcgctgccca gtccctcgcc gtgatgaaca cgctggggct ggatccggcc
      181 aaggtcaacc ccatgggcgg cgccatcgcc ctgggccacc cgctgggcgc caccggggcg
      241 atacgcgccg ccaccgtggt gcatgcgctg cgccgccaca agctgaagta cggcatggtg
      301 accatgtgcg tgggcacggg ccagggcgcg gccggcatct tcgaaagcct ttgaggccag
      361 ggtcgcccgc atgacgcggg ccccgcccca aaccgtgcac acttgggtgc catgactcac
      421 gccacacatc ctttcgacga cgcgctggcc cttgaccccg gcgcgcccga ccatttcagc
      481 ggccgcacca cgcccggcta ctggaacatg gtcgggccct atggcggcat cacagcggcc
      541 acgctgctgc aggcggtgct gcgccacccg ctgctgctgg gcgagccgct gtcgctcacc
      601 gtgaactacg ccgccgccgt ggccgagggg ccatttaccg tgcaggccac gccggtgcgc
      661 accaaccgct ccacccagca ctggatggtg acggtgcagc aggccaatgc cggcggcgag
      721 cagatggtga ccaccaccgc caccgtggtc accgccgcac ggcgcgagac ctggggcgtg
      781 agcgaccagc ccatgcccac cgtgcctgcg ccggcgcaat gccagcgcac cccgccgatg
      841 ttccgtgtgg agtggctgaa ccgctatgag atgcgccccg tcacgggcga gctgcccacg
      901 cagtgggacg acagcggcca caccagcctg acgcagctgt ggctgcgcga cgcgcctgag
      961 cgcgcgctgg actttgcggc gctggccgcc atgtgcgacg tgttctaccc gcgcgtctgg
     1021 ctgcgccgcg cgcgccatgt gccggcgggc acggtgtcga tcaccgtgta ctttcatgcc
     1081 ggcagtgcgc tgctggccca gaccggtgac ggctatctgc tggcccaggc gcgtgcgcag
     1141 gagtttcgca acggcttctt cgaccagtcg gcccagctgt ggaaccaggc cggcagcctg
     1201 ctggccacca gccaccagat cgtttactac aaggaatgaa cgccatgagc agcacgcaag
     1261 acatgctggt gcacaccgag gctggtgtga ccacgctgac cttcaaccgc gccgacaaga
     1321 aaaactccat caccgaggcc atgtatgccg ccatggccga cgccttggcg caggccgcgc
     1381 aggacgccgc cgtgcgctgc gtcgtgttcc agggcgacat ggccatcttc agcgccggca
     1441 acgacattgc cgactttctg cagcagtcct cgaaaggcgc cgcgcccgag cttgcgggcg
     1501 agcgcccggt gtggcgcttt ctgcgcctgc tggcgggctt ccccaagccc ctggtggcgg
     1561 ccgtctgcgg cccggccgtg ggcattggca ccacgctgct gttgcattgc gacctggtct
     1621 acgcgggcga caacgccgcg ttctcgttgc ccttcgtgaa cctgggcctg tgccccgagg
     1681 cggcctccag cctgctgttg ccgcagatgc tgggctacca ccgggcggcc gaggccctgc
     1741 tcttgggcga gcccttcatg gccgaggcgg cgctggaggt gggcctggtg aaccgcgtcg
     1801 tgcctccggc tgaatgcaac gccctggccc aggcccaggc gcgcaagctg gcggccaaac
     1861 ccctgtccgc gctgatcgag accaagcgcc tgatgaaggc cggccaggcc agcgaggtgc
     1921 aggcgcgcat gcaggaagag ggggcgagct ttgcccgcct gatggccggc cccgccgcca
     1981 aggaggcctt caccgccttc atgcagaaac gcaagccgga cttcagcagt tgctgaggcc
     2041 cttgccgctg caggtgccgg ctgcgggtga tgcaaaattt gtagcattcg cagcgcttgc
     2101 tgtgtgatgc tgcaagccat gaaagcgcat gcaacgaacg agcggtttgg ttaccgttct
     2161 tgcgcaatca gggcggtttt gctgcgctgc aacttctacg atccgtggtg tttttatcag
     2221 gagatccgaa cccatggccg tgcaaaaccc cttctttggc aaacgtgaca acgatgcgtt
     2281 ccaggcccgt caaccctcgc ccgtgcccgg cggcagcaac gtgaccggtc agaccagcac
     2341 cagcaccacc aaccccagca ccgccagcag catgaacacc agcaagcccg cagcgtccag
     2401 caccaccgag agcggcggca gcaagctcac cgtgggcccc aacatcaagc tcaagggcgt
     2461 ggagatcacc gattgcgaca ccctggtggt ggagggcacg gtggaggcga ccatggactc
     2521 gcgcgtgatc cagatcaccg agcagggcgc gtttcgcggc acggccgaga tcgacattgc
     2581 cgagatccat ggcgagttcg acggctcgct caccgtgcgc gagaagctgg tgatcttcag
     2641 caccggcaag gtgacgggca agatccgcta cggcaaggtg gtgatcgagg agggcggcca
     2701 gttgtcgggc gagatttcgg tcggcgtggg cggcgcccgc gcgccagcgg catccacctc
     2761 tgctgtgacc agcagcacca gcagcgcggc atcctcgtcc accctggcgg cggcctgacc
     2821 tgccaccggc gatcggcttg gctatcaggt gcgcagcagc agcgcgtaca cggccacgcc
     2881 gccgtagccc agccccaggg cggcgcacac caggtacagc gccacgctga accagtacct
     2941 cagcggctca tcgtcgcgtt caaaagtacg ccagccgcgc ggctggcgca cctcgccgct
     3001 gtgcagcgcc tgccgctcca ggtgcaggca ccaaccccgg cgcccagcac agcagcaggg
     3061 cccagagcat catgcgggca gctgcgcggg cgctatcggg cgcggccggc cctggtcgtc
     3121 aatggccaca taggtcaggc gcgcctcggt caccttgaca tgctgaccct gggcgcgaat
     3181 atgctcggcg tacacctcca cctgcaccgt gatcgaggtg ttccccaggc gggtcacgct
     3241 ggagaagaac gacagcaggt cgcccacgcg caccggctgt ttgaagatga actcgttcac
     3301 cgccacggtg gccatgcgcc cctggatcag gcgcgccggc agcaccgagc cggccagatc
     3361 gacctgcgcc atcacccaac cgccgaagat gtcgccgttg ccgttggtgt ccgccggcat
     3421 gggtatgacc ttgagcacca gctccttgtc ggtgggcagt tgcgtggcgg cgctgaggcg
     3481 atcctgggac atgggcacaa tctcgggctt gttttgactc ctttgattgt cctccatgcg
     3541 ccgccacggc gattcctcgc cccccatgtc cgcggccgca gagcccaaaa cccgatccga
     3601 cgccgccacg ctggcgcggc tgctgcccta cctgtgggaa tacaaatggc gcgtggccgc
     3661 ggccattgcc ttgatgctgg gcgcaaaaat ggccaatgtg ggcgtgccgc tgctgctcaa
     3721 gcagctggtg gacgccatga ccatcgcgcc aggggcgccg gcggcgctgc tggtcgtgcc
     3781 cgtgggcctg ctgctggcct atggggcgct gcgcttcacc aattcgctgt tcaacgaact
     3841 gcgcgagctg gtgtttgcca aggccacgca tggcgcggcg cgtgccattg cgctgcgcac
     3901 cttcgagcat ctgcatgcgc tctcgctgcg ctttcacctg gagcgccaga ccggcggcat
     3961 gacgcgcgac atcgagcgcg g
//
LOCUS       DAOMTQ010000219         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6136_length_3978_cov_30.364517, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000219 DAOMTQ010000000
VERSION     DAOMTQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6136_length_3978_cov_30.364517"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PK518_14780"
     CDS             <1..624
                     /locus_tag="PK518_14780"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM50223.1"
                     /translation="GMSGPNAPLRRYWQAMTHPRWAGDVGLLGRPHDLGNISAYRGSP
                     TGLQDYMGYLSANFDPSISWKDLEWIRAFWKGPMVIKGILDPEDAKDAVRFGADGIIV
                     SNHGGRQLDGVLSSARALPAIADAVKGQIKILADSGIRNGLDVVRAIALGADCAMIGR
                     AYIYALATAGEAGVKHLLELLEKEMRVAMTLTSVAKVADITGELLVS"
     gene            complement(704..2665)
                     /gene="htpG"
                     /locus_tag="PK518_14785"
     CDS             complement(704..2665)
                     /gene="htpG"
                     /locus_tag="PK518_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRM50224.1"
                     /translation="MSKHTHSFQAEVAQLLHLVTHSLYSNKEIFLRELVSNASDACDK
                     LRFEALNDATLYEDQSNLEVRVSFDKAARTLTIQDNGIGMSTQEAIDHLGTIAKSGTK
                     DFMSRLSGDQKQTASEQGQLIGQFGVGFYSGFIVADKITVESRRAGLKANEGVRWTSS
                     GAGDFEVEQIERAARGTSVILHLRGDAEEYLNAWQLKQIIGKYSDHISLPILMEKEEW
                     KEGEKEGDAGAMVKTGEWETVNKASALWSRPKKDITTEQYQDFYKSISHDHEAPLSWS
                     HNRVEGNTEYTQLLYIPAKAPFDLWNRDKKAGVKLYVKRVFIMDDAEALMPQYLRFVK
                     GVIDSADLPLNVSRELLQESRDVRLIRDGSVKRVLSMLEDLAKHDKHEASESADGVTD
                     VVSDEDKAKEGKYSQFYAEFGAVLKEGLGEDFANRERLAKLLRFASTSSDTASVSLQD
                     YKARMKEGQEAIYYITADTLAAAKNSPQLEVFKKKGIEVLLMTDRVDEWALNYLHDFD
                     GTPLQSVAKGAVDLGKLQDEAEKKAAEEAAEAFKPLLAKLKEALKDKAEDVRVTTRLV
                     DSPACLVVQDDGMSQQLARMLKQAGQSAPETKPVLEVNPEHALVKKLDGSVHFHDLAH
                     ILFDQALLAEGGLPEDPAAYVKRVNALLV"
     gene            complement(2782..3534)
                     /locus_tag="PK518_14790"
     CDS             complement(2782..3534)
                     /locus_tag="PK518_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM50225.1"
                     /translation="MSQELSLHQRRRPPTAQELAGIPWLARLQPSERERAVAAVVVGD
                     ADPGDYVCRVGRPVTYWFGVVQGLLKMNTDTVDGASMTLAGLPPSGWFGEGTAVKREP
                     YRYNVQALRKSVVAGLPMDSFHWLLDHSIGFNRFVMGQLNERLGQFIAVREIDRLTNP
                     DLRVARSLAALFHPVLFPGVGEVLRITQQELANLVGLSRQRVNEALAVLQEQGAIRVE
                     YGGLRVLDLPALRSSPFVRATVVAGALNQPAH"
     gene            complement(3666..>3978)
                     /locus_tag="PK518_14795"
     CDS             complement(3666..>3978)
                     /locus_tag="PK518_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HRM50226.1"
                     /translation="AATAPESAQAQAPEPKPARPAPQATQAPVFAQADLPPAVREQLP
                     TLQLAGVTYSTNPLYRMVIVNGQVLHEGDQAAPGLVLERIEPGRTVWAFRGYRYALPS
                     Q"
BASE COUNT          598 a         1310 c         1343 g          727 t
ORIGIN      
        1 ggcatgagcg gccccaacgc cccgctgcgc cgctactggc aggccatgac gcacccccgc
       61 tgggcggggg acgtgggcct gctgggccgc ccgcacgacc tgggcaacat ctcggcctac
      121 cgcggcagcc ccacgggcct gcaggactac atgggctacc tcagcgccaa cttcgacccc
      181 tcgatctcgt ggaaggatct ggagtggatc cgcgccttct ggaaggggcc gatggtcatc
      241 aagggcatcc tcgaccctga ggatgcgaag gatgcggtgc gcttcggcgc cgacggcatc
      301 atcgtctcca accacggcgg gcgccagctc gacggcgtgc tgtcgtcggc gcgcgcgctg
      361 ccggccatcg ccgacgcggt gaagggccag atcaagatcc tggccgactc gggcatccgc
      421 aacgggctgg acgtggtgcg cgccatcgcc ctgggggccg actgcgccat gatcggccgc
      481 gcctacatct acgcgctggc cacggccggc gaggccggcg tcaagcacct gctggagctg
      541 ctggaaaagg aaatgcgcgt ggccatgacc ctgaccagcg tggccaaagt ggcggacatc
      601 acgggcgaac tgctggtgtc ctgaattggc gctgcagccc cgcagccccg cagccccgca
      661 gccctacagc atggcggcca gcgccagcgg gctttggctc acatcacacc aatagcgcgt
      721 tcacccgttt gacgtaagcc gccggatcct ccggcaggcc accctcggcc agcaacgcct
      781 ggtcgaacag gatgtgcgcc aggtcgtgga agtgcacgct gccgtcgagc ttcttcacca
      841 gcgcatgctc ggggttcacc tccagcacgg gcttcgtctc gggcgcgctc tggccggcct
      901 gcttgagcat gcgggccagt tgctggctca tgccgtcgtc ctgcaccacc aggcaggcgg
      961 gtgaatcgac caggcgggtg gtgacgcgca catcctcggc cttgtctttc aacgcttcct
     1021 tcagcttggc gagcaggggc ttgaaggcct cggcggcctc ttccgcggcc tttttctcgg
     1081 cctcgtcctg cagcttgccc aggtccaccg cgcccttggc gacggactgc agcggcgtgc
     1141 cgtcaaagtc gtgcaggtag ttcagcgccc attcgtccac gcggtcggtc atgagcagca
     1201 cctcgatgcc cttcttcttg aagacttcga gctgcgggct gttcttggcg gcggccaggg
     1261 tgtcggcggt gatgtagtag atggcctcct ggccctcctt catgcgcgcc ttgtagtcct
     1321 gcaggctgac gctggccgtg tcgctgctgg tgctggcaaa gcgcagcagc ttggccaggc
     1381 gctcgcggtt ggcgaagtcc tcgcccaggc cttccttgag cacggcgccg aactcggcgt
     1441 agaactggct gtacttgcct tccttggcct tgtcctcgtc gctcaccaca tcggtcacgc
     1501 cgtcggcgga ttcgctggcc tcgtgcttgt catgcttggc caggtcttcc agcatggaga
     1561 gcacgcgctt gaccgagccg tcgcggatca ggcgcacgtc gcggctttcc tgcagcagct
     1621 cacggctcac gttcagcggc aggtcggcgg agtcgatcac gcccttgacg aagcgcaggt
     1681 actgcggcat cagcgcctcg gcgtcgtcca tgatgaagac gcgcttgacg tacagcttca
     1741 cgccggcctt cttgtcgcgg ttccacaggt cgaacggggc cttggcgggg atgtacaaga
     1801 gctgcgtgta ctcggtgttg ccctcgacgc ggttgtggct ccaggacagc ggcgcctcgt
     1861 ggtcgtggct gatggacttg tagaagtcct ggtactgctc ggtggtgatg tccttcttgg
     1921 ggcgcgacca cagggcgctg gccttgttca ccgtctccca ttcgccggtc ttcaccatgg
     1981 cgcccgcatc gccctctttc tcgccctcct tccattcctc tttctccatg aggatgggca
     2041 ggctgatgtg gtcggagtat ttgccgatga tctgcttgag ctgccaggcg ttcaggtact
     2101 cctccgcgtc gccccgcagg tgcaggatga cgctggtgcc gcgcgccgcg cgctcgatct
     2161 gctcgacctc gaagtcgccc gcgccgctgc tcgtccagcg cacgccctca ttggccttca
     2221 ggcccgcgcg gcggctttcc acggtgatct tgtcggcgac gatgaagccc gagtagaagc
     2281 ccacgccgaa ctggccgatc agctggccct gctcgctggc ggtctgcttc tggtcgcccg
     2341 agaggcggct catgaagtcc ttggtgccgc tcttggcgat ggtgcccagg tggtctatgg
     2401 cctcctgcgt gctcatgcca atgccgttgt cctggatggt cagggtgcgc gcggccttgt
     2461 cgaaggacac gcgcacctcc aggttggact ggtcttcata cagcgtggcg tcgttcagcg
     2521 cctcaaagcg cagcttgtcg caggcgtcgg acgcgttgga gaccagctcg cgcagaaaga
     2581 tttccttgtt ggagtacagc gagtgcgtga ccaggtgcag cagttgcgcg acttcggcct
     2641 ggaaggaatg ggtgtgcttg ctcatgcgtg ttgttgcgtc gaatgaatca gagaaagtga
     2701 gtgccggcac agccggaacg cccctgcata tgggggcgca atggtgctct tcaagggggc
     2761 gccgcagcca gctgccgcgc gtcagtgtgc gggctggttg agcgcacccg ccaccaccgt
     2821 ggcacgcaca aacgggctgc tgcgcagcgc cggcaggtcg agcacgcgca ggccgccgta
     2881 ttcgacgcgg attgcgccct gctcctgcag cacggccagg gcctcgttga cgcgctggcg
     2941 cgacaggccg accaggttgg ccagctcttg ctgggtgatg cgcagcacct cgcccacgcc
     3001 cggaaacagc accgggtgga acagcgccgc caggctgcgc gccacgcgca ggtcggggtt
     3061 ggtgaggcgg tcgatctcgc gcacggcgat gaactggccc aggcgctcgt tgagctgacc
     3121 catgacaaag cggttgaagc cgatggagtg gtccagcagc caatggaagc tgtccatggg
     3181 caggccggcg accacgctct tgcgcagggc ctgcacgttg tagcggtagg gctcgcgctt
     3241 gacggccgtg ccttcgccaa accagccgct gggcggcagg ccggccagcg tcatgctggc
     3301 gccgtccacg gtgtcggtgt tcatctttag caggccctgc accacgccaa accagtaggt
     3361 cacgggccgg ccgacgcggc agacatagtc gccagggtcg gcatcgccca ccaccacggc
     3421 ggccacggcg cgctcacgct ccgagggctg cagccgcgcc agccagggaa tgccggccag
     3481 ctcctgggcg gtgggcgggc ggcgccgctg gtgcagggag agttcttggc tcatgcctgc
     3541 gaagcatgcg tgcaaagtgc ctgaaccggc agcgggaata ccacgaaagc gccaatgatt
     3601 agtactattt tgatagctgt taacgcttta cagaaaagcg ctggagccta atttatacaa
     3661 caactctact gggatggcag cgcatagcgg tagccacgaa acgcccaaac cgtgcgccct
     3721 ggctcaatgc gctcgagcac caggccgggt gctgcctggt cgccctcgtg cagtacctgg
     3781 ccattgacga tgaccatgcg gtacagcggg ttcgtggaat aggtcacccc ggccagctgc
     3841 agcgtgggta gctgctcgcg cacggcgggc ggcaggtcgg cctgggcgaa taccggcgcc
     3901 tgggtggcct gcggggccgg ccgcgcgggc ttgggttcgg gcgcctgcgc ctgcgcggac
     3961 tcgggggccg tggcggcg
//
LOCUS       DAOMTQ010000220         3909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6296_length_3909_cov_27.716399, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000220 DAOMTQ010000000
VERSION     DAOMTQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6296_length_3909_cov_27.716399"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PK518_14800"
     CDS             complement(<1..865)
                     /locus_tag="PK518_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM50227.1"
                     /translation="MKSNSSGPAPPLAPEPGDGVRPDGLPQLIAATSQAEPGQADALA
                     RARAFAEPLLIGETLETGENTLAHADAVAAILNALGSSETMQAAAYLVYTCNHLNKPE
                     EVIGKAFGSNFAALAVETTKLMRVQTQAREAQVAGLQVDDAATQNENVRKMLLAFSRD
                     LRVVMLRLASRLQTLRYYAASKRPVSPAIAREAQQVFAPLANRLGIWQMKWELEDLAF
                     RFLEPDTYREVARLLDEKRVERELYMEQLRARLESELRAHSISASVSGRPKHIYSIVK
                     KMRGKSLNFDQV"
     gene            complement(862..1803)
                     /locus_tag="PK518_14805"
     CDS             complement(862..1803)
                     /locus_tag="PK518_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM50228.1"
                     /translation="MIQPTLNYVPCLGPAAAGGAAEQGHRMAYWEWNATGNPAHPHVI
                     VCVHGLTRQGRDFDHLARHLSRQARVICPDVAGRGESDWLADPMAYQVPVYAADMLTL
                     LLQLHQQAPMAMLDWVGTSMGGLIGMGVCGMPDLPLPVPVRRLVLNDVGPRIEWAALQ
                     RIGEYLGQPLRFDSLQQAADALWQISSSFGPHTPEQWLDLSRAMVRPAPGGGFKLHYD
                     PAIALPFKALTPEAASAGEQQLWQLYDQISAQVLLLRGAQSDLLSAHTAQEMGQRGPR
                     ARLVEFADVGHAPTLVNAGQIQVVADFLFDRQQGPGA"
     gene            1873..2655
                     /locus_tag="PK518_14810"
     CDS             1873..2655
                     /locus_tag="PK518_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HRM50229.1"
                     /translation="MLNGKTALVTGSTSGIGLGIAKALARQGANIVLNGFGDVDGPRA
                     EVLAAGQGHGVRVQHHGADMSRAGDIEDMMKFSAAQFGRVDILVNNAGIQHVASVQDF
                     PAERWDAIIAINLTSAFHTTRLALPAMQAANWGRIINVASVHGLVASAQKSAYVAAKH
                     GIVGLTKVTALENATTGVTCNAICPGWVLTPLVQKQVDAKAAEHGLSNEDAKKLLLGE
                     KEPSMQFTTPEELGELAVFFCSAAANNVRGVAWNMDGGWAAQ"
     gene            complement(2717..3391)
                     /locus_tag="PK518_14815"
     CDS             complement(2717..3391)
                     /locus_tag="PK518_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913875.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRM50230.1"
                     /translation="MGVTTVFAQYSLTPAPQDVLQLSATGAVEVQQDLLVLTLSATRE
                     AADAAAVQTQLKQALDGALGLARAAAQPQQLEVRTGAFGMYPRHGKDGKISGWQGRAE
                     LVLQGRDFARITATAGKIQSMAISQIAFDLSREARARVESEAQAKAIEAFKGRATELA
                     RGFGFTGYTLREVSVSSNEMAPGPRPRMLAMEAKAMSVASDAPVPVEAGKAQVTVTLS
                     GSVQLR"
     gene            3641..>3909
                     /locus_tag="PK518_14820"
     CDS             3641..>3909
                     /locus_tag="PK518_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM50231.1"
                     /translation="MASTTNRTDKILVVDDDARIRDLLRRYLTQEGFEVMVAEDGKAL
                     NRILLRETVDLIVLDLMMPGEDGLSICRRLRAASDRTPIIMLTAKG"
BASE COUNT          672 a         1327 c         1266 g          644 t
ORIGIN      
        1 acacctggtc gaagttgagc gacttgccgc gcatcttctt gacgatgctg tagatatgct
       61 tgggccggcc cgagacgctg gcgctgatgc tgtgggcgcg cagctcggat tccagccggg
      121 cgcgcagctg ctccatgtac agctcacgct cgacgcgctt ttcgtccagc agccgtgcca
      181 cctcccggta ggtatcgggc tccaggaagc gaaacgccag gtcttccagc tcccacttca
      241 tctgccagat gcccaggcgg ttggccaggg gcgcgaacac ctgctgcgcc tcgcgggcaa
      301 tggccggaga gacggggcgc ttgctggccg cgtagtagcg cagcgtctgc aggcgcgatg
      361 ccaggcgcag catgaccacg cgcaggtcgc gcgagaaggc cagcagcatc ttgcgcacgt
      421 tttcgttctg cgtggccgcg tcgtccacct gcagaccggc cacctgcgcc tcgcgcgcct
      481 gggtctgcac gcgcatgagc ttggtggtct ccacggccag cgccgcgaag ttgctgccaa
      541 aggccttgcc aatgacctct tcaggcttgt tcaggtggtt gcaggtatag accagatagg
      601 ccgccgcctg catggtctcg gaactgccca gcgcattcag aatggcggcc acggcatcgg
      661 catgggccag ggtgttttcg ccggtctcca gggtttcgcc gatgagcagc ggctcggcaa
      721 aggcgcgggc acgcgccagc gcgtcggcct ggccgggctc ggcctgcgag gtggcggcaa
      781 tcagctgagg caagccatca gggcgtacgc cgtcacccgg ctccggggcc aggggcggcg
      841 caggaccgga ggagttgctt ttcatgctcc cggcccctgt tgccgatcga acagaaagtc
      901 ggcgaccacc tggatctgcc cggcgttgac cagggtgggt gcatggccca cgtcggcaaa
      961 ctcgaccagt cgcgctcgtg ggccacgctg tcccatttcc tgggcggtat gcgcactcag
     1021 cagatccgac tgcgcgccgc gcagcagcag tacctgggcg ctgatctggt cgtacagctg
     1081 ccacagctgc tgctccccgg cgctggccgc ctcgggcgtc agggccttga atggcagggc
     1141 gatcgccggg tcgtaatgca gcttgaaacc gccgccgggt gcgggccgca ccatggcgcg
     1201 cgacagatcc agccactgct cgggggtgtg cgggccaaaa ctgctcgaaa tctgccacag
     1261 cgcatcggcg gcctgctgca gcgagtcgaa acgcagcggc tgccccaggt actcgcctat
     1321 gcgctgcagt gccgcccact cgatgcgcgg ccccacgtcg ttgagcacca ggcgccgcac
     1381 gggcaccggc agcggcagat cgggcatgcc gcaaaccccc atgccgatca gccctcccat
     1441 gctggtgccc acccagtcca gcatggccat gggcgcctgc tggtgcagtt gcagcaacag
     1501 ggtcagcatg tcggcggcgt acaccggcac ctggtaggcc atggggtcgg ccagccagtc
     1561 gctctcgccc cgacccgcca cgtccgggca gatgacgcgc gcctggcggc tcagatggcg
     1621 tgccagatgg tcgaagtcgc gtccctggcg cgtcaggcca tgcacgcaga cgatgacatg
     1681 cgggtgcgcg ggattgcccg tggcattcca ctcccagtag gccatgcggt ggccctgttc
     1741 ggcagccccg cctgcagcgg cggggcccag gcacggtacg tagttcagcg taggttgaat
     1801 catggaaacg gtaccgtcat acagcaatcg ataatccctg cgctgcatcg taattcattc
     1861 ggagagactc tcatgctgaa cggcaaaacc gccctcgtca ccggctccac cagcggcatc
     1921 ggccttggca tcgccaaggc cctggcgcgc cagggggcaa acatcgtgct caacggcttt
     1981 ggcgacgtgg acggcccacg cgccgaggtg ctggccgccg gccagggcca tggggtgcgc
     2041 gtccagcacc acggcgcgga catgagccgc gcgggcgaca tcgaggacat gatgaagttc
     2101 agcgccgccc agttcggccg cgtggacatt ctggtgaaca acgccggcat tcagcatgtg
     2161 gccagcgtgc aggacttccc tgcggagcgc tgggacgcca tcatcgccat caacctgacc
     2221 agcgccttcc acaccacgcg cctggccctg ccggccatgc aggcggccaa ctgggggcgc
     2281 atcatcaacg tggcctcggt gcatggcctg gtggcctcgg cgcagaaatc ggcctatgtg
     2341 gcggccaagc atggcatcgt cggcctgacc aaggtgacgg cgctggagaa cgccaccacc
     2401 ggcgtgacct gcaacgccat ctgccccggt tgggtcctca cccccctggt gcaaaagcag
     2461 gtggacgcca aggccgccga gcatggcctg tccaacgaag acgccaagaa gctgctgctg
     2521 ggcgagaaag agccctcgat gcagttcacc acgccggagg agctgggcga gctggccgtg
     2581 ttcttctgct cggccgctgc caacaacgtg cgcggcgtgg cctggaacat ggatggcggc
     2641 tgggcggccc agtaagcagt ttaactattg tttttatagc tgtcatcgcc ctactattgg
     2701 acgctagcgg ccaaaatcac cgcaactgca ccgaacccga aagggtgacc gtcacctgcg
     2761 ccttgccggc ctccaccggc accggcgcgt cactggccac ggacatcgcc ttggcctcca
     2821 tggccagcat gcgcggacgc gggccggggg ccatctcgtt gctgctcacg ctcacctcac
     2881 gcagggtgta gccggtaaag ccaaagccac gcgccagctc ggtggcgcgc cccttgaagg
     2941 cctctatggc cttggcctgc gcctcgctct ccaccctggc gcgcgcctcg cgcgagaggt
     3001 cgaacgcgat ctggctgatg gccatgctct ggatcttgcc tgccgtggcg gtgatgcgcg
     3061 caaagtcccg cccctgcagc accagctcgg cgcgcccctg ccagccgctg atcttgccgt
     3121 ccttgccatg gcgcggatac atgccaaacg cccccgtgcg cacctccagc tgctgcggct
     3181 gggccgcggc gcgcgccaga ccgagcgcgc catccagggc ctgcttgagc tgcgtctgca
     3241 cggccgccgc atcggccgcc tcgcgggttg cgctcaatgt caataccagc agatcctgct
     3301 gcacctcgac ggccccggtg gccgaaagct gcagcacatc ctgcggcgca ggcgtcaggc
     3361 tgtattgggc aaaaacggtt gtaacgccca tcagtaaagc gccaacagct acatttttta
     3421 aagcaagtct catatccatc tttctcctgg aatggggaat tgaccaccat cgcaacgata
     3481 agccagcgcg cctgcgacgg cgctggaagg acccgtttac gcccatgccg gcctgggcaa
     3541 aacctgtaac agttggtcag gttcccgagg aaaacgcggt ttttggcgcg caagctggtc
     3601 agaatcggcg ttgttacaaa ttggactggg gaaagacagc atggcttcaa caaccaaccg
     3661 caccgacaag atcctggtgg tggacgacga tgcgcgcatc cgcgacctgc tgcgccgcta
     3721 tctgacgcag gaaggcttcg aggtcatggt ggcggaagac ggcaaggcgc tcaaccgcat
     3781 cctgctgcgc gagacggtgg acctgatcgt gctcgacctg atgatgcccg gcgaggacgg
     3841 cctgtccatc tgcaggcgcc tgcgcgccgc cagcgaccgc acgcccatca tcatgctcac
     3901 cgccaaggg
//
LOCUS       DAOMTQ010000221         3850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6442_length_3850_cov_29.617391, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000221 DAOMTQ010000000
VERSION     DAOMTQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_3850_cov_29.617391"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..>3850
                     /locus_tag="PK518_14825"
     CDS             135..>3850
                     /locus_tag="PK518_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HRM50232.1"
                     /translation="MSHFLDRLSYFSQPRETFSNGHGETNGEDRTWERAYRDRWAHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRPDLPNHEPRGCARGASYSWY
                     LYSANRVKYPLVRGRLLKHWRAALAVAKSPVDAWAAIVQNQEARREWQRQRGLGGFVR
                     SSWDEVNQMIAAANVYTIKQYGPDRVIGFSPIPAMSMISYAAGARYLSLIGGVPLSFY
                     DWYCDLPPSSPQTWGEQTDVPESADWYNSNFIIAWGSNVPQTRTPDAHFFTEVRYKGA
                     KIVAVTPDYSEVAKLSDLWLHPKQGTDAAVAMAMGHVILKEFYFDRRSAYFDEYARRY
                     TDLPLLVLLKEKQLPDGRTAMVPDRYVRASDFAGNLGQANHPDWKTVAYGLDGKVALP
                     NGSIGFRWGKEGRPDEGLWNLEDKDARSGNDVKLKLSVLEDGAQAHAVADVAFPYFGG
                     VQTPHFQANDQGGDVMLRRVPITHLDLHGEDAQGRVAVATVFDLLAGNYGIARGLDGE
                     ALDGSYDANAPYTPAWQESITGVPREHVITVARQFADNADKTQGKSMVIIGAGMNHWY
                     HCDMNYRSIINMLMLCGCIGQSGGGWSHYVGQEKLRPQTGWTALTFALDWIRPPRQQN
                     ATSFFYAHTDQWRYEKLGVDEVLSPLADKAAYQGSMIDYNVRAERMGWLPSAPQLQAN
                     PLQLARDAQERGLDPKDYVVQSLKNGSLSLSCEDPDNPLNWPRNLFVWRSNLLGSSGK
                     GHEYFLKHLLGTQNGVQGKDLGPQDAKPEEVKWHASAPEGKLDLLVTLDFRMSTTCLY
                     SDIVLPTATWYEKNDLNTSDMHPFIHPLSAAVDPAWQARSDWEIYKGFAKAFSEVCVG
                     HLGVEKEVVLTPIMHDTPGELAQPFGVKDWKRGEIDLIPGVTAPQVTVVERDYPNTYA
                     RFTALGPLMDRLGNGGKGIGWNTQTEVEQLGDLNGRVREEGVAQGRPRIVSDIDATEV
                     VMMLAPETNGHVACKAWEALGKQTGRDHVHLALHREDEKIRFRDIQAQPRKIISSPTW
                     SGLESEKVSYNAGYTNVHELIPWRTLTGRQQFYQDHPWMRDFGEGLCSYRPPVDLKTL
                     HEVAGKKPNGHPELQLNFITPHQKWGIHSTYSDNLHMLTLNRGGPVVWISEEDAKRGG
                     IVDNDWIELFNANGAIAARAVVSQRVKPGMVLKYHAQEKTINTPGSEITGTRGGIHNS
                     VTRVVLKPTHMIGGYAQYSYGFNYYGTIGTNRDE"
BASE COUNT          752 a         1316 c         1227 g          555 t
ORIGIN      
        1 tgccttgctg acaagaggtt gagcgttgag ggttgagcgt tttgcgcaaa cccaacgccc
       61 aacgcccaac gcccaacgcc caacgcccaa cgcacaacgc acaacgcaca acgattgaac
      121 caaggagcac cacgatgagt cactttctcg accgtctctc gtatttttcg cagccacgcg
      181 aaaccttctc caacggccat ggcgagacca atggcgaaga ccgcacctgg gagcgcgcct
      241 accgcgaccg ctgggcgcat gacaagatcg tgcgctccac ccatggcgtg aactgcaccg
      301 gctcctgctc ctggaagatc tacgtcaagg gcggcatcgt cacctgggaa acccagcaga
      361 ccgactaccc ccgcacgcgc cccgacctgc ccaaccacga gccccgtggc tgcgcgcgcg
      421 gcgccagcta cagctggtac ctgtacagcg ccaaccgcgt gaaatacccg ctggtgcgcg
      481 ggcgcctctt gaagcactgg cgcgcggcgc tggccgtggc caagagcccg gtcgatgcct
      541 gggccgccat cgtgcagaac caggaggcgc gccgcgaatg gcaaaggcag cgcggcctgg
      601 gcggctttgt gcgctcgagc tgggacgagg tgaaccagat gattgccgcg gccaacgtct
      661 acaccatcaa gcagtacggg ccggaccgcg tcatcggctt ctcgccgatt ccggccatgt
      721 ccatgatctc gtacgcggcg ggcgcgcgct acctgagcct gatcggcggc gtgccgctgt
      781 cgttctacga ctggtactgc gacctgccac cgtccagccc ccagacctgg ggcgagcaga
      841 ccgacgtgcc cgagtcggcc gactggtaca actccaactt catcatcgcc tggggctcca
      901 acgtgccgca gacgcgcacg cccgacgcgc atttcttcac cgaggtgcgc tacaagggcg
      961 cgaagatcgt cgccgtcacg cccgactatt ccgaggtcgc caagctctcc gacctgtggc
     1021 tgcaccccaa gcagggcacg gatgcggccg tggccatggc catgggccat gtgatcctca
     1081 aggagttcta cttcgacagg cgctcggcct acttcgacga gtacgcgcgc cgctacaccg
     1141 acctgccgct gctggtgctg ctcaaggaga agcagctacc cgacggccgc acggccatgg
     1201 tgcccgaccg ctatgtgcgc gccagcgact tcgccggcaa cctgggccag gccaaccacc
     1261 ccgactggaa gaccgtggcc tacggcctgg acggcaaggt ggcgctgccc aacggctcca
     1321 tcggctttcg ctggggcaag gagggccgtc ccgacgaggg cctgtggaac ctggaggaca
     1381 aggatgcgcg cagcggcaac gacgtgaagc tcaagctctc ggtgctggaa gacggtgccc
     1441 aggcgcatgc ggtggccgac gtggccttcc cctacttcgg tggcgtgcag acgccgcatt
     1501 tccaggccaa cgaccagggt ggtgacgtga tgctgcgccg cgtgcccatc acccacctgg
     1561 atctgcacgg cgaggacgcc cagggccgcg tggccgtggc cacggtgttc gacctgctgg
     1621 ccggcaacta cggcatcgcg cgcggcctgg acggcgaggc cctggacggc agctacgacg
     1681 ccaatgcgcc ctacaccccg gcctggcagg agagcatcac cggcgtgccg cgcgagcatg
     1741 tgatcaccgt ggcgcgccag ttcgccgaca acgccgacaa gacccagggc aagagcatgg
     1801 tcatcatcgg cgccggcatg aaccactggt accactgcga catgaactac cgcagcatca
     1861 tcaacatgct gatgctgtgc ggctgcatag gccagtcggg tggcggctgg tcgcactacg
     1921 tgggccagga gaagctgcgc ccgcagaccg gctggacggc gctgaccttt gcgctggact
     1981 ggatacgccc gccgcgccag cagaacgcca ccagcttctt ctacgcccac accgaccagt
     2041 ggcgctacga gaagctgggt gtcgatgagg tgctctcgcc gctggcggac aaggccgcct
     2101 accagggcag catgatcgac tacaacgtgc gcgccgagcg catgggctgg ctgcccagtg
     2161 cgccgcagct gcaggccaat ccgctgcagc tggccaggga tgcgcaggag cgcggcctgg
     2221 accccaagga ctacgtggtg cagtcgctga agaacggctc gctgtccttg agctgcgaag
     2281 acccggacaa cccgctgaac tggccgcgca acctgtttgt ctggcgctcc aacctgctgg
     2341 gcagctcggg caaggggcat gagtatttct tgaagcacct gctgggcacg caaaacggcg
     2401 tgcagggcaa ggatctgggc ccgcaggatg ccaagcccga ggaggtgaag tggcatgcca
     2461 gcgcacccga gggcaagctc gatctgctgg tgacgctgga cttccgcatg agcaccacct
     2521 gtctgtactc cgacatcgtg ctgcccacgg ccacctggta cgagaagaac gacctgaaca
     2581 cctcggacat gcaccccttc atccacccgc tgtcggccgc tgtcgatccg gcctggcagg
     2641 ccagaagtga ctgggagatc tacaagggct tcgccaaggc cttcagcgag gtctgcgtgg
     2701 gccacctggg ggtggaaaaa gaggtggtgc tcacgcccat catgcacgac accccgggcg
     2761 agctggcaca gccgtttggc gtcaaggact ggaagcgcgg cgagatcgac ctgatccccg
     2821 gcgtgaccgc gccgcaggtc acggtggtcg agcgcgatta ccccaacacc tatgcgcgct
     2881 tcacggccct gggccccttg atggacaggc tgggcaatgg cggcaagggc attggctgga
     2941 acacccagac cgaggtcgag caactgggcg acctgaacgg ccgcgtgcgc gaggagggcg
     3001 tggcgcaggg caggccgcgc atcgtgagcg acatcgacgc caccgaggtg gtgatgatgc
     3061 tcgcgcccga gaccaacggc catgtggcct gcaaggcctg ggaggccttg ggcaagcaaa
     3121 ccgggcgcga ccatgtgcac ctggcgctgc accgcgagga cgagaagatc cgctttcgcg
     3181 acatccaggc gcagccgcgc aagatcatca gctcgcccac ctggtcgggg ctggaatccg
     3241 agaaggtcag ctacaacgcc ggctacacca acgtgcacga gctgatccca tggcgcaccc
     3301 tcaccggccg ccagcagttc taccaggacc acccctggat gcgcgacttt ggcgagggcc
     3361 tgtgcagcta ccgcccgcct gtcgatctga agaccctgca cgaggtggcc ggcaagaaac
     3421 ccaatggcca ccccgagctg caactgaact tcatcacgcc gcaccagaaa tggggcatac
     3481 acagcacgta cagcgacaac ctgcacatgc tcacgctcaa ccgcggcggc ccggtggtct
     3541 ggatcagcga ggaggacgcc aagcgcggcg gcatcgtgga caacgactgg atagagctgt
     3601 tcaacgccaa tggcgcgatc gccgcgcgcg cggtggtcag ccagcgcgtc aagcccggca
     3661 tggtgctgaa ataccacgcg caggaaaaga ccatcaacac ccccggctcg gagatcaccg
     3721 gcacgcgcgg cggcatccac aactcggtca cgcgcgtggt cctcaagccc acgcacatga
     3781 tcggcggcta cgcgcagtac agctacggct tcaactacta cggaaccatt ggcaccaacc
     3841 gcgacgagtt
//
LOCUS       DAOMTQ010000222         3845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6454_length_3845_cov_25.930079, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000222 DAOMTQ010000000
VERSION     DAOMTQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6454_length_3845_cov_25.930079"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1412)
                     /locus_tag="PK518_14830"
     CDS             complement(<1..>1412)
                     /locus_tag="PK518_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRM50233.1"
                     /translation="DAGAEVLRNWYALRDMDDGCANAAAEMLAARQVKPLDIWRKARL
                     AAEANRLRVARKAVEVVAPEALAPLREALDAPAKYLKGHATARGRERQELVVLALVRM
                     AMSDAGVAASLLENKWGVHLSAEERNWLWGVIGKQAALSLSPDAAGYFGNVSRDADLN
                     DDLLAWKVRAALRAGQWKQVGRAIDAMSPTARQDSTWTYWKARSLTAGRAGDEERAQA
                     RQLYERIAGSTGFYEQLALEELGERVTPPPAPAPLTDAEKAAARANPGLQRALYAIAM
                     GLRAEGVREWNYSTNLHQAGGMAERELLAAADLACQQQVWDRCINTSERTKTVIDAGQ
                     RFPMPFRSAVVERAQGVGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWT
                     AKKIGLTGFTPSQINDRDTNITIGTAYLKLALDDFDGSMALAAAAYNAGPGRPRNWRN
                     GPVLDAAIWAENVPFNETRD"
     assembly_gap    1413..1512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1513..1724)
                     /locus_tag="PK518_14835"
     CDS             complement(<1513..1724)
                     /locus_tag="PK518_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRM50234.1"
                     /translation="MQWLKILTPLLTTAVLAASAPWAAAQNRGDDTLLEMQQAFRKGD
                     RQRLTTLLPGARGHVLEPWAAYWELRA"
     gene            1740..2312
                     /locus_tag="PK518_14840"
     CDS             1740..2312
                     /locus_tag="PK518_14840"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRM50235.1"
                     /translation="MDKAALRRDLIAQRLSLPDRLLRADQLQSVMRIWLVDRPDTVIG
                     AYWPIKGEFDPLPALHRWKEDGELLDQPQLRRIGLPVVNKAHKTLTFHAWYPGCPMEE
                     DAYGIPKPKDTEPIHPTLLFVPCVGYGPGGYRLGYGGGFYDRTLATLQPRPTTVGLGY
                     THGYLDEFEPEAHDLPLDAILNDNGVVWPV"
     gene            2391..3077
                     /locus_tag="PK518_14845"
     CDS             2391..3077
                     /locus_tag="PK518_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRM50236.1"
                     /translation="MLQLYIGNKNYSSWSMRPWVLLRQAGIAFEEVSVRFDSFDAGSQ
                     FKRQLQAVSPAGKVPVLVDGDTAIWDSLAIAEYLAETYPDKQLWPQDKAARARARSVT
                     AEMHSGFTALRSHCGMNIEARLPEVGAILWRDQAGVRADVQRLIDMWGELLAQHGGPM
                     LFGQFTIADAFYAPVCMRLATYALPVPQPIADYVRRVQQLPGVKAWIDGALAERDFLD
                     FEEPYRIRRA"
     gene            3091..>3845
                     /locus_tag="PK518_14850"
     CDS             3091..>3845
                     /locus_tag="PK518_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRM50237.1"
                     /translation="MKLHILSDLHLGVQGMEHPQTDADIVVLAGDIARPAQAVAWAQG
                     FGKPVLYVPGNHEFYGASHAQVLAELQRLSQGTRVQLLDNRSVTLAGVRFVGSTLWSD
                     FRIFASEEQRQQAMEQAQRFMYDFSRIRMGPEPGDALFTPQDAAALYDANLLWLRAAL
                     AEPFDGPTVVITHHAPTLNSIHPRFAGSPLNGIFVSDAQDLVAASGARLWIHGHTHDS
                     FDYQVQGTRVLCNPRGYAKEGKVENALFDARLVV"
BASE COUNT          573 a         1322 c         1264 g          586 t
ORIGIN      
        1 tagtcgcgcg tctcgttgaa gggcacgttc tcggcccaga tggcggcgtc cagcaccggg
       61 ccgttgcgcc agttgcgcgg gcggcccggg ccggcgttgt aggcggcggc ggccagcgcc
      121 atggagccat cgaaatcatc cagcgccagc ttcaaatacg ccgtgcctat ggtgatgttg
      181 gtgtcgcggt cgttgatctg gctgggcgtg aagcccgtca ggccgatctt tttcgccgtc
      241 cagcgcgccg tggccggcat cacctgcatc aggcccgagg cgcccacgcc cgagcgcgcg
      301 tccatgatga agcggctttc ctgccggatc aggccataga cataggccgg gtccaggccg
      361 acgccctgcg cccgctcgac cacggcactg cgaaacggca tcggaaagcg ctggccggcg
      421 tcgatcacgg tcttggtgcg ttcgctggtg ttgatgcagc ggtcccacac ctgctgctgg
      481 caggccaggt cggccgcggc cagcagctcg cgctcggcca tgccgccggc ctggtgcagg
      541 ttggtgctgt agttccattc gcgcacgccc tcggcgcgca ggcccatggc gatggcgtac
      601 agggcacgct gcaggccagg attggcgcgc gcggcggcct tctccgcatc ggtgagcggc
      661 gcgggcgcgg gcggcggcgt gacgcgctcg cccagctcct ccagcgccag ctgctcgtaa
      721 aagcccgtgc tgccggcgat gcgctcgtac agctgacgcg cctgggcgcg ctcttcgtcg
      781 ccggcacggc ccgccgtgag cgagcgcgcc ttccagtagg tccaggtgct gtcctggcgc
      841 gctgtggggc tcatggcgtc gatggcgcgg cccacctgct tccactggcc ggcgcgcagc
      901 gcggcgcgca ccttccaggc cagcaggtca tcgttcagat ccgcgtcacg gctgacgttg
      961 ccaaagtagc cggcggcatc gggcgacagc gacagcgccg cctgcttgcc gatcacgccc
     1021 cagagccagt tgcgctcctc ggccgacagg tgcacgcccc atttgttctc cagcaggctg
     1081 gcggcgacgc cggcgtcgct catggccatg cgcaccaggg ccagcaccac cagctcctgg
     1141 cgctcgcggc cgcgcgccgt ggcgtggccc ttgaggtatt tggcgggcgc gtccagcgcc
     1201 tcgcgcagcg gtgccagggc ctcgggggcg acgacctcca ccgccttgcg cgccacgcgc
     1261 aggcggttgg cctcggcggc caggcgcgcc ttgcgccata tatccagcgg cttgacctgc
     1321 ctggcggcca gcatctcggc ggcggcgttg gcgcagccgt cgtccatgtc gcgcagcgcg
     1381 taccagttgc gcagcacctc ggcgccggcg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nngcgcgcag ctcccagtag gcggcccagg gctccagcac atggccgcgc
     1561 gcgccaggaa gcagcgtggt cagcctttgc ctgtcgccct tgcgaaaggc ctgctgcatc
     1621 tccagcaagg tgtcgtcgcc acggttttgc gccgccgccc agggggcgga ggcggcgagc
     1681 accgccgtgg tcagaagggg tgtcagaatc ttgagccatt gcatgggggg aattatcgga
     1741 tggacaaagc agccctacgc cgcgatttga ttgcacaacg cctgagcctg cccgaccggc
     1801 tgctgcgcgc cgaccagctg caaagcgtca tgcgcatctg gctggtggac cggccagaca
     1861 ccgtcatcgg cgcctactgg cccatcaagg gcgagttcga cccgttaccg gccctgcacc
     1921 gctggaagga ggacggcgag ctgctggacc agccccagct gcgccgcatc ggcctgccgg
     1981 tggtgaacaa ggcgcacaag accctcacct tccacgcctg gtacccgggc tgcccgatgg
     2041 aggaggacgc ctacggcatc cccaagccca aggacaccga acccatccac cccacgctgc
     2101 tgttcgtgcc ctgcgtgggc tacgggccgg gcggctaccg cctgggctat ggcggcggct
     2161 tctacgaccg taccctggcc acgctgcagc cgcgccccac caccgtgggc ctgggataca
     2221 cccacggcta cctggacgaa ttcgagcccg aggcgcacga cctgccgctg gacgccatac
     2281 tgaatgacaa cggcgtggtc tggccggtgt gaaagaaaag tggctgtaac gcaatacagc
     2341 aaagcgccga cagctcttat ttttgaagca tcaacacaaa gggatgcacc atgctccagc
     2401 tctacatagg caacaaaaac tattcctcct ggtccatgcg gccctgggta ctgctgcgcc
     2461 aggccggcat tgccttcgag gaggtgtcgg tgcgctttga cagctttgat gccggctcgc
     2521 agttcaagcg ccagctgcag gccgtcagcc ccgcgggcaa ggtgccggtg ctggtggatg
     2581 gcgacaccgc catctgggac agcctggcga ttgccgaata cctggccgag acctaccccg
     2641 acaagcagct gtggccccag gacaaggccg cacgcgcccg cgcgcgcagc gtgacggccg
     2701 agatgcacag cggcttcacc gcgctgcgta gccattgcgg catgaacatc gaggcgcggc
     2761 tgcccgaggt gggcgccatc ctctggcgcg accaggccgg cgtgcgcgcc gacgtgcagc
     2821 gcctgatcga catgtggggc gagctgctgg cgcagcatgg cgggcccatg ctgttcggcc
     2881 agttcacgat tgccgatgcc ttctacgcgc cggtgtgcat gcgcctggcg acctacgccc
     2941 tgcccgtgcc gcagcccatc gccgactatg tgcggcgcgt gcagcagctg cccggcgtca
     3001 aggcctggat cgacggcgcg ctggccgagc gggacttcct ggacttcgag gaaccctacc
     3061 gtatccgccg cgcctgacgg cccatcgtcc atgaaactgc acatcctctc cgacctgcac
     3121 ctgggcgtgc agggcatgga gcacccgcag accgatgccg acatcgtcgt gctggccggc
     3181 gacattgccc gccccgcgca ggccgtcgcc tgggcccagg gctttggcaa gccggtgctg
     3241 tatgtaccgg gcaaccacga gttctacggc gccagccacg cccaggtgct ggccgaactg
     3301 cagcggctga gccagggcac gcgggtgcaa ctgctggaca accgcagcgt cacgctggcc
     3361 ggcgtgcgct ttgtgggctc cacgctgtgg agcgactttc gcatctttgc cagcgaagaa
     3421 cagcgccagc aggccatgga gcaggcgcag cgcttcatgt acgacttcag ccgcatccgc
     3481 atgggccccg agcccggcga cgcgctgttc accccgcagg acgcagcagc cctgtacgac
     3541 gccaacctgc tgtggctgcg cgcggcgctg gccgagccgt ttgacggccc cacggtggtc
     3601 atcacgcacc atgcgcccac gctcaacagc atccacccgc gctttgccgg ctcgccgctg
     3661 aatggcatct tcgtctcgga cgcgcaggac ctggtggcgg ccagcggcgc gcggctgtgg
     3721 atacacggcc atacgcacga cagctttgac taccaggtac agggcacgcg cgtgctgtgc
     3781 aacccgcgcg gctacgccaa ggagggcaag gtggagaacg cgctgttcga cgcgcggctg
     3841 gtggt
//
LOCUS       DAOMTQ010000223         3833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6488_length_3833_cov_26.033351, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000223 DAOMTQ010000000
VERSION     DAOMTQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6488_length_3833_cov_26.033351"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK518_14855"
     CDS             complement(<1..515)
                     /locus_tag="PK518_14855"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRM50238.1"
                     /translation="MPATLSPTPLCQLLGCRLPLLLAGMGGVSRHELVAAVTEAGGFG
                     FLGMVREPLALIEREVAQLRARTDRPFGVNLIPAGTPAALLQSQVDLCIALGVPVVGL
                     FWDVDRPLIARLRAAGITVVHQVGSLQDALDAQDAGAQALIAQGVEAGGHVRGRQPLM
                     QLLAELLPHARV"
     gene            complement(589..1989)
                     /locus_tag="PK518_14860"
     CDS             complement(589..1989)
                     /locus_tag="PK518_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM50239.1"
                     /translation="MSSPDSPGTPSHHAGPSRAGFSSSLGVLAATLGSAVGLGNIWKF
                     PSLTGANGGAGFLLVYLLSTLLIGLPLMVAEIAIGRQARANPISALRQLAPRGAPWWL
                     VGLGGMAAAFLILSFYSEVVAWVFAYVAKALAGSLLSADPAQTQASFDALVTDPVQSL
                     LWQWVVLAFIGSILLLGVTKGIEAVTKKLMPLLFLLLLLLCGYSLTLPGAGLALSFLF
                     APEWHKITPAVVLTAMGLAFFKLSIGMGTMMTYGSYFTPGQNIPATATRVMLADLSVS
                     LLAGMAIFPAVFSFGFAPSAGPSLVFITIPAVFAQIPGGQVLLAAFFILTAIAATGAM
                     LSIMEVPVVILHERWGLARPRATLLTLLILMLLGTGCALSQSLLAHIRPGGRTLFDWA
                     DYLSSNLLMPMGGIAIALFVGWAWGEQAFAAQLSNQGQLHNRALARALCLLLRWVTPV
                     LIGLVMLAGLGVFGSA"
     gene            complement(2202..3413)
                     /locus_tag="PK518_14865"
     CDS             complement(2202..3413)
                     /locus_tag="PK518_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRM50240.1"
                     /translation="MVQSVLGSLVLGYRPLWNAARKLAGVQLYVHGHGSQRVDAAHLL
                     RTIQELWSAASAPLLLSPRSQALLIDLLQHAPRGAPWIAVPGDWLALPAVRENVQRAH
                     GRGLRLVWSGTLSRLPDADTARLFDNSLLQLSGSDAAAALQAAGGKQPSPLIAGQMYD
                     NVASRALLSQCLDQGRALAVAGWPMEDVLHSLRHQPLAPAHEVVQKLLKAIDDDQSID
                     RLEQILGQDPLLAHRFLVYTNSAALGLRTGVDSLRRGLVMMGYGSLSRWLSDQLPHCA
                     TEPDLRPIRSAMVLRARLTEQLLQAGAGTELRGEVYLAGLFSQLDELLHENLGAILHR
                     LPLSERIYNAAVLRTGAYAPSLELACALEGDSAGAIVQLCEAHELELEDINRALLRVL
                     SELAVERPGGH"
     gene            complement(3478..>3833)
                     /locus_tag="PK518_14870"
     CDS             complement(3478..>3833)
                     /locus_tag="PK518_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRM50241.1"
                     /translation="SPSLMSSIDSAIKDITNATNSTQAAQAVSQALAHMDAGIEQLSN
                     IRGYAGELLNRADRITGDQESRSIQLEADRSRAEDLDMLKGISDFQNANVGLEAALKS
                     YAQVQRLSLFNYVNG"
BASE COUNT          630 a         1283 c         1395 g          525 t
ORIGIN      
        1 acgcgcgcgt ggggcagcag ctcggccagc agctgcatca gcggctggcg cccgcgcaca
       61 tggccgccgg cctccacgcc ctgggcgatg agggcctggg cgccggcgtc ctgggcgtcc
      121 agcgcgtcct gcagcgagcc cacctggtgc accacggtga tgccggcggc gcgcagccgc
      181 gcgatcaggg gccggtccac gtcccagaac aggccgacca cgggcacgcc gagcgcaatg
      241 cacagatcca cctggctctg cagcagcgcg gcgggcgtgc cggcggggat gaggttgacg
      301 ccaaagggcc ggtcggtgcg tgcgcgcaac tgggccacct cgcgctcgat gagcgccagc
      361 ggctcgcgca ccatgcccag gaagccaaag ccgccggcct cggtgacggc ggccaccagc
      421 tcatggcgcg acacgccgcc catgccggcc agcagcagcg gcaggcggca gcccagcagc
      481 tggcacagtg gggtggggct gagggtggct ggcatggcat ctcctgttgg cggctgacgg
      541 gccggccatg atgcggcgcc atcgccagtg gtgcaagcgc cccggacatc aggcgctgcc
      601 aaacaccccc agcccggcca gcatgaccag cccgatcagc accggcgtga cccagcgcag
      661 cagcaggcac agtgcgcgcg ccagggcgcg gttgtgcagc tggccctggt tggacagctg
      721 cgcggcaaag gcctgctcac cccaggccca gccgacgaag agggcgatgg cgatgccgcc
      781 catgggcatg agcaggttgg acgagaggta gtcggcccag tcgaacagcg tgcgcccgcc
      841 ggggcggata tgggccagca ggctctggct cagggcgcag cccgtgccca gcagcatcag
      901 gatcagcagc gtgagcagcg tggcccgggg ccgcgccagg ccccagcgct cgtgcagtat
      961 caccaccggc acctccatga tggacagcat ggcgccggtg gcggcaatcg cggtcaggat
     1021 gaagaacgcc gccagcagca cctggccgcc cggtatctgc gcgaacaccg cggggatggt
     1081 gatgaacacc agcgacggcc ctgccgaggg cgcgaagcca aagctgaaca ccgccggaaa
     1141 gatggccatg ccggccagca gcgacaccga caggtcggcc agcatcacgc gcgtggcggt
     1201 ggccggaatg ttctgcccgg gcgtgaagta gctgccgtag gtcatcatcg tgcccatgcc
     1261 gatggacagc ttgaagaacg ccagccccat ggcggtaagc actacggcgg gcgtgatctt
     1321 gtgccattcg ggcgcgaaca ggaacgacag cgccaggccc gcgcccggca gcgtcaggct
     1381 gtagccgcac agcaaaagca gcagcaggaa cagcagtggc atcagcttct tggtcaccgc
     1441 ctctatgccc ttggtcacgc ccagcagcag gatggagccg atgaaggcca gcaccaccca
     1501 ttgccacagc agcgactgca ccgggtctgt gaccagggcg tcgaagctgg cctgggtctg
     1561 tgccgggtcg gcggacagca ggctgccggc gagcgccttg gccacatagg caaacaccca
     1621 ggcgacgacc tcggagtaga acgacaggat caggaaggcc gccgccatgc cccccaggcc
     1681 gaccagccac cagggcgcgc cgcggggcgc gagctggcgc agcgccgaga tcgggttggc
     1741 gcgcgcctgg cggccgatgg cgatctcggc caccatcagc ggcaggccga tgagcagcgt
     1801 ggacagcaga tagaccagca aaaagcccgc cccgccgttg gcgccggtga gcgaggggaa
     1861 cttccagata ttgcccagcc cgacggccga gcccagcgtg gcggcgagca cgcccaggct
     1921 ggaggaaaag ccggcgcggc tggggccggc atggtgcgac ggcgtgccgg gggagtctgg
     1981 tgaactcatg atttgatagc tgtttgcgtc cagtgtaccg gcgctgtggg ctaaaaaggc
     2041 ctgactatgt acatgccgca gaggcccctc acccctccct ctccccaaag gggcgagggc
     2101 gatcggttgc gccggttttt actccctctc cctcttgggg gagggtgggg gtgagggccg
     2161 ccaccgtatc tgaggcatgt cgccagtcag gcaaaaaggc ctcaatgccc gccgggccgc
     2221 tccaccgcca gctcggacag cacgcgcagc agcgcgcggt tgatgtcttc cagctccagc
     2281 tcatgcgcct cgcacagctg gacgatggcg ccggcgctgt cgccctcgag cgcgcaggcc
     2341 agctccaggc tgggggcgta ggcgccggtg cgcagcacgg cggcgttgta gatgcgctcg
     2401 gacagcggca ggcggtgcaa tatggcgccc aggttctcgt gcagcagctc gtcgagttgc
     2461 gagaacaggc cggccagata cacctcgccg cgcagctcgg tgccggcgcc ggcctgcagc
     2521 agctgctcgg tcaggcgcgc gcgcagcacc atggcgctgc gtatcgggcg caggtcgggc
     2581 tcggtggcgc agtgcggcag ctggtcggac agccagcgcg agagcgagcc gtagcccatc
     2641 atcaccaggc cgcggcgcag cgaatccacc cccgtgcgca ggcccagcgc ggccgaattg
     2701 gtatagacca ggaagcggtg ggccagcagc gggtcctggc ccaggatttg ctccaggcgg
     2761 tctatcgact ggtcatcgtc tatggccttg agcagcttct gcaccacctc gtgcgccggc
     2821 gccagcggct ggtggcgcag gctgtgcagc acatcctcca tgggccagcc ggccacggcc
     2881 agggcacggc cctggtccag gcattgcgag agcagcgcgc ggctggccac gttgtcgtac
     2941 atctgcccgg cgatcagcgg gctgggctgc ttgccaccgg ctgcctgcag cgcggcggcg
     3001 gcatcgctgc cggacagctg cagcaggctg ttgtcgaaca ggcgcgcggt gtcggcgtcg
     3061 ggcaggcggc tcaaggtgcc gctccacacc aggcgcaggc cgcggccatg ggcgcgttgc
     3121 acgttctcgc gcacggcggg cagcgccagc cagtcgcccg gcaccgctat ccagggcgcg
     3181 ccgcgcgggg cgtgctgcag caggtcgatg agcagcgcct gcgagcgcgg cgacagcagc
     3241 agcggcgccg atgcagcgct ccacagctcc tggatggtgc gcagcaggtg cgcggcatcg
     3301 acccgctggc tgccatggcc atgcacgtac agctgcacgc cggcgagctt gcgcgcggca
     3361 ttccatagag ggcggtagcc cagtaccagg ctgcctagga cggattggac catggggtgg
     3421 ggtcaggcaa tggagatgat ggctgcgatt gtcgcgcctt cgaacatgct ccaggcccta
     3481 tccgttgacg tagttgaaca gcgacaggcg ctgcacctgg gcataggact tgagggccgc
     3541 ctccaggccg acgttggcgt tctggaagtc ggagatgccc ttgagcatgt ccaggtcttc
     3601 ggcgcgcgag cggtcggcct ccagctggat ggaacggctt tcctggtcgc ccgtgatgcg
     3661 gtcggcgcgg ttgagcagct cgccggcgta gccgcggatg ttgctcagct gctcgatgcc
     3721 ggcatccatg tgcgccaggg cctggctcac ggcctgtgcg gcctgcgtgc tgttggtggc
     3781 gttggtgatg tccttgatgg cgctgtcgat ggagctcatc aggctggggc tgg
//
LOCUS       DAOMTQ010000224         3764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6677_length_3764_cov_23.472365, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000224 DAOMTQ010000000
VERSION     DAOMTQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6677_length_3764_cov_23.472365"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="murB"
                     /locus_tag="PK518_14875"
     CDS             complement(<1..979)
                     /gene="murB"
                     /locus_tag="PK518_14875"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRM50242.1"
                     /translation="MLVEKNVPLQPYNSFGIAARAHTLVRVRSAQDVHELLADHRLAR
                     QPLFVLGGGSNIVLTGDVKPVVLKMEIKGRRLLEETDKAWIVEAGAGEPWHDTVDWTL
                     TQGWPGLENLALIPGTVGAAPVQNIGAYGVELQDRFHSLVAIDLATGQPFTLDAAQCA
                     FGYRDSVFKHAAPLAEQEGAGLPGHWGRGMGLAGRAVITHVRFLLRKDWAPELGYLDL
                     ERKRIEAGVARPTARQIFDWVCEIRRAKLPDPAVIGNAGSFFKNPTVSPEQCQDIIAR
                     DPKIVHYPMADGSIKLAAGWLIDACGWKGKSVGKAGVYDKQALVLVNRGT"
     gene            1048..1533
                     /locus_tag="PK518_14880"
     CDS             1048..1533
                     /locus_tag="PK518_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRM50243.1"
                     /translation="MPSFDTVCEANFVEVKNAVENTAKEIGTRFDFKGTSAGVELKDK
                     EITLFGDAEFQLQQVEDILRGKLSKRSVDVRFLDVQKPQKIGGDKLKQVVKVRNGIDS
                     EQAKKIQKLIKESKLKLQAAIQEEKVRVTGAKRDDLQAAMALIRKEVADLPLTFDNFR
                     D"
     gene            1547..2191
                     /locus_tag="PK518_14885"
     CDS             1547..2191
                     /locus_tag="PK518_14885"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRM50244.1"
                     /translation="MRYPLTCLSTALAFCLPAQVQAQDVALAGILGGKALLVVNGSAP
                     RGVAPGESHMGVQVVSVGREDAVVDSAGGRRSLRLGEAPVRVGGSGTGQRVVLKADAR
                     GHFVSSGQINGRIMQYMVDTGASTVAIGRPDAQRMGLKFEEQGQSVRMNTANGVAQGW
                     RLRLDSVRVGDVELRGVDAIVTPQPMPYVLLGNSFLREFEMSRNGDEMVLLKRR"
     gene            2218..3216
                     /locus_tag="PK518_14890"
     CDS             2218..3216
                     /locus_tag="PK518_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HRM50245.1"
                     /translation="MTAQSPANAPDTVDSEYEDLLGQWSDLESALSVLLARPRSVQDF
                     AFKLRQCDRWLQDLVAHDIDAALYLMFQLAATSSVGYSAAHALVCATLCHILGHELRL
                     PVQERDSLVRAAFTMNLAMTALQDELAQQREPLTPAQRSAVDQHPRAGLDLLEELGIS
                     DELWLEVVGQHHTPQPEADALAGLSALERLTRILGAIDRYAAMISPRKSRAGRSATDS
                     VRAIVGQDADVRDEVGLTLVRTVGLCPPGTFVRLDNGETAIVLRRGERANFPLVASVV
                     DAKGEPQSEPRLYHTERGQQRIQAALARSAVTVELPYRTMVRLGLYAAQRSASVSG"
     gene            complement(3231..>3764)
                     /gene="plsY"
                     /locus_tag="PK518_14895"
     CDS             complement(3231..>3764)
                     /gene="plsY"
                     /locus_tag="PK518_14895"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRM50246.1"
                     /translation="PRSYGSKNPGATNVLRSGSKAAAVVTLLLDAAKGWLPVALVRWF
                     GAPHGLEEGTMAMVGLAAFAGHLWPVFFRFEGGKGVATALGVLLGVSGWLGLATLLTW
                     VIVAFFFRYSSLASLVAAAFAPFFYFLGAGMVWYSDARLGVAMVVMAGLLAWRHKANI
                     QRLIQGKESRLGAKKKA"
BASE COUNT          640 a         1314 c         1254 g          556 t
ORIGIN      
        1 gcgtgccgcg gttcaccagc accagggcct gtttgtcgta cacccctgcc ttgcccacgc
       61 tcttgccctt ccagccgcag gcgtctatca accagcccgc cgccagcttg atgctgccgt
      121 ccgccatggg gtagtgcacg atcttcgggt cgcgcgcgat gatgtcctgg cactgctcgg
      181 gcgacaccgt ggggttcttg aagaagctgc cggcgttgcc gatgacggcc gggtcgggca
      241 gcttggcgcg gcgtatctcg cacacccagt cgaagatctg ccgcgccgtg ggccgcgcca
      301 cgccggcctc gatacgcttc ctctccagat ccaggtagcc cagttcaggc gcccagtcct
      361 tgcgcagcag aaagcgcaca tgggtgatca ccgcccggcc cgccagcccc atgccccggc
      421 cccagtggcc gggcaggccg gcgccctcct gctcggccag cggcgcggca tgcttgaaca
      481 ccgagtcgcg gtagccgaag gcgcattgcg cggcgtccag cgtgaagggc tggcccgtgg
      541 ccaggtcgat cgcgaccagc gaatgaaagc ggtcctgcag ctccacgcca taggcgccaa
      601 tgttctgcac cggcgccgcg cccacggtgc cggggatcag ggccaggttc tccagccccg
      661 gccagccttg ggtcaaagtc cagtccacgg tgtcgtgcca gggctcgccg gcgccggcct
      721 cgacgatcca ggccttgtcg gtttcttcca gcaggcgccg gcccttgatc tccatcttca
      781 gcaccacggg cttcacatcg cccgtgagca cgatgttgct gcccccgccc agcacaaaca
      841 gcggctggcg cgccaggcgg tggtcggcca gcagctcatg cacatcctgc gccgagcgca
      901 cgcgcaccaa cgtatgggcg cgcgcggcaa tgccgaagct gttgtagggc tgcaggggga
      961 cgtttttctc gactaacatc ggccggattg tcgcacccgc cccactgggc tgcgggtttg
     1021 ccccaggttt gaccagagag tattgccatg ccgtcttttg ataccgtttg tgaagccaac
     1081 ttcgtcgaag tgaagaatgc cgtggagaac acggccaagg agataggcac gcgctttgac
     1141 ttcaagggca cgtcggccgg cgtggagctc aaggacaagg agatcacgct gtttggcgac
     1201 gccgaatttc agctgcagca ggtcgaggac attctgcgcg gcaagctcag caagcgcagc
     1261 gtggacgtgc gcttcctcga cgtgcaaaag ccgcagaaga tcggcggcga caagctcaag
     1321 caggtggtca aggtgcgtaa cggcatcgac agcgagcagg ccaagaagat ccagaagctc
     1381 atcaaggaaa gcaagctcaa gctgcaggcc gccattcagg aagaaaaggt gcgcgtgacc
     1441 ggcgccaagc gcgacgacct gcaggcggcc atggcgctga tccgcaagga agtggccgac
     1501 ctgccgctca cattcgacaa cttccgcgac tgactttccc cgcaccatgc gctacccgct
     1561 gacctgcctg agcaccgcgc tggcgttttg cctgccggcg caggtgcagg cgcaggacgt
     1621 ggccctggcc ggcattctgg gtggcaaggc gctgctcgtc gtcaatggca gcgcgccgcg
     1681 cggcgtggcg ccgggcgagt cccacatggg ggtgcaggtt gtctcggtgg gccgcgagga
     1741 tgccgtggtg gacagtgccg gcggccggcg cagcctgcgc ctgggcgagg cgccggtgcg
     1801 cgtcggcggc agcggcacgg gccagcgcgt ggtgctgaag gccgatgcgc gcgggcattt
     1861 cgtcagcagt ggccagatca acggccgcat catgcagtac atggtggaca ccggcgcctc
     1921 cacggtcgcc ataggccggc ccgacgcgca gcgcatgggc ctcaagttcg aggaacaggg
     1981 ccagagcgtg aggatgaaca ccgccaacgg cgtggcccag ggctggcgcc tgcggctgga
     2041 ttcggtgcgc gtgggcgatg tcgaactgcg cggcgtggac gccatcgtca ccccccagcc
     2101 catgccctat gtgctgctgg gtaacagctt cctgcgcgaa ttcgagatga gccgcaacgg
     2161 cgacgaaatg gtgctgctca agcggcgctg aaccacccca cacaaaccac cccgcccatg
     2221 accgcacagt cacccgccaa cgccccggat acggtcgata gcgagtacga agacttgctg
     2281 ggccagtggt cggatctgga gtccgcgctg tcggtgctgc tggcgcgccc gcgcagcgtg
     2341 caggactttg cgttcaagct gcgccagtgc gaccgctggc tgcaggacct ggtggcgcac
     2401 gacatagacg ccgccctgta cctgatgttc cagctcgccg ccacctccag cgtgggctac
     2461 agcgccgcgc acgccctggt gtgcgccacg ctgtgccaca tacttggaca cgagctgcgc
     2521 ctgcccgtgc aggagcgcga cagcctggtg cgcgcggcct tcaccatgaa cctggccatg
     2581 acggcgctgc aggacgaact ggcccagcag cgcgagccgc tcacgcccgc gcagcgcagc
     2641 gccgtcgatc agcacccgcg cgcgggcctg gacttgctgg aggagctggg catcagcgac
     2701 gagctgtggc tggaggtggt gggccagcac cacacgccac agcccgaagc cgacgccctg
     2761 gccggcctgt cggcgctgga gcggctgaca cgcatccttg gcgccataga ccgctacgcg
     2821 gccatgatca gcccgcgcaa gtcgcgcgcc ggccgcagcg ccaccgattc ggtgcgggcc
     2881 atcgtcggcc aggatgccga cgtgcgcgac gaggtcggcc tgaccctggt gcgcaccgtg
     2941 ggcctgtgcc cgcccggcac cttcgtgcgg ctcgacaatg gcgagacggc catcgtgctg
     3001 cgccgcggcg agcgcgccaa ctttccgctg gtggccagcg tcgtcgatgc caagggggag
     3061 ccgcaatcag aaccccggct gtaccacacg gagcgcggcc agcagcgcat tcaggcggcg
     3121 ctggcgcgct cggcggtcac cgtggagctg ccctaccgca ccatggtgcg cctggggctg
     3181 tacgcggcgc agcgcagcgc cagcgtctcg ggctgatcag gccttctttt tcaggccttc
     3241 ttttttgccc ccaggcgcga ctccttgccc tggatgagcc gctgaatgtt cgccttgtgg
     3301 cgccaggcca gcaggcccgc catgaccacc atggccacgc ccagccgcgc atcgctgtac
     3361 cagaccatgc cggcgcccag aaagtaaaag aacggcgcaa aagccgccgc caccagcgac
     3421 gccagcgagg aatagcggaa gaagaacgca acgatgaccc aggtcagcag cgtggccagg
     3481 cccagccagc cgcttacgcc cagcagcacg cccaacgccg tggccacgcc cttgccgccc
     3541 tcgaagcgga agaacaccgg ccacagatgg cccgcaaacg ccgccaggcc gaccatggcc
     3601 atggtgccct cctccagccc atggggcgcg ccaaaccagc gcaccagggc caccggcagc
     3661 cagcctttgg cggcgtccag cagcagcgtg acgacggccg ccgccttgga gccagagcgc
     3721 agcacattcg tagcgccggg gttcttgctg ccatagctgc gcgg
//
LOCUS       DAOMTQ010000225         3758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6690_length_3758_cov_25.214691, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000225 DAOMTQ010000000
VERSION     DAOMTQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6690_length_3758_cov_25.214691"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK518_14900"
     CDS             complement(<1..205)
                     /locus_tag="PK518_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRM50247.1"
                     /translation="MHKAISVTVIGAGLMGHGIGQVFARAGHEVVITDPNATVLNMAL
                     ERVEANLVSEGVDVNRVLCNMRVD"
     gene            complement(218..1159)
                     /locus_tag="PK518_14905"
     CDS             complement(218..1159)
                     /locus_tag="PK518_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRM50248.1"
                     /translation="MAKPQDKVIISCAVTGAMHTPTMSPYLPITPDQIAEQSIEAAEA
                     GAAILHLHARNPVDGSPTPDPKIFDQFVPRIKAATDAVINITSGGSTRMTLEERLAYP
                     LMAKPEMCSLNMGSMNFSIHQAAHKITDWKHPWEKPYVEGMEDLIFRNTFKDIKHILQ
                     TLGDGCGTRFEFECYDIGHLYNLAYFIDEGLVKGPLFIQSIYGILGGVGPDPENLVMM
                     RTTADRLFGRENYRFSVLGAGRHQMSLLTMGAVMGGNVRVGLEDSLYLAKGQLAHTCA
                     DQVRKIRRILEELSLEIATPDEARAMLGLKGKDSVGF"
     gene            1437..2459
                     /locus_tag="PK518_14910"
     CDS             1437..2459
                     /locus_tag="PK518_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM50249.1"
                     /translation="MSAKPWINEKIYAPYKLAALVETLTELGVPSQMSLQGTGLALCD
                     VENPETKTSVGQYFTACRNAVRTSKTPAAPFLVGRRMHLSAYGMYGYALMCSLTLRDF
                     FNEGVKYHKLATPTVVIEWREQDGAAIWSFPRLVAPELSVDLFQFLIEQQLVQHAIHL
                     NDVAGPECRPLRALLSYPAPAHEHLYAEYLGCPAYFGSETCELHYDAAILDQPTQLAH
                     KLTSALMQATCERLIGQAKTTVGISGQVYQILMASPEQLPRMEDVAAMVHMNERTMRR
                     KLESEGTSFGQIIDDVRASLAAEYLKTTKMTTEDIAALVGFSDAANFRRAFKRWTGKT
                     PSEYRT"
     gene            complement(2597..>3758)
                     /locus_tag="PK518_14915"
     CDS             complement(2597..>3758)
                     /locus_tag="PK518_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRM50250.1"
                     /translation="LVPALGTQQALLAIVAGSIIGAGLLGWVAKLGCDSGLASAGLMH
                     AVYGRGFASLPILLNIVQLVGWGTFELVVMRDATVAIGRQSGLLAAAWWPVAATLLWG
                     GVVALLISGSMVRLVRRVIARVALPLVVLSLIWLSWQFLSMAQAQGLDEIWQRRGAGG
                     MGVMPALDLVIAMPVSWLPLVADYARHGVSGAGALRGSWLGYALANIWCYCLGVLVAL
                     TLPSQDLVTALLLAQGGLLALSLILIDEVDNAYGDAYSGAVSAHSLRPGWSVRRWGLV
                     LAALCTGLALVLPMHSLEPFLLLLSSVFVPLFGVILGRLAFGVAAPQRLLDARAVHGL
                     PVALWLGGIACYHLLPRVLPGLGAALPTLALCLVLAWLTRPRGACSTRRSTH"
BASE COUNT          715 a         1231 c         1177 g          635 t
ORIGIN      
        1 cgtcgacgcg catgttgcac aacacgcgat tcacatcgac cccctcggag actaggttgg
       61 cttccacgcg ttccagagcc atgttgagca ccgttgcgtt gggatcggtg atcacgacct
      121 cgtggccggc tcgcgcaaac acctggccga tgccgtgtcc catcaggcca gcgccaatca
      181 cggtgacgga gattgctttg tgcatgtcgg ccctcgctca gaagccgacg ctgtctttgc
      241 ccttaaggcc cagcatcgcg cgggcttcgt caggcgtggc gatctccaac gacagctctt
      301 cgagaatgcg ccggatcttg cgcacctgat ccgcacatgt gtgcgccagt tggcctttgg
      361 cgaggtacag gctgtcctcc agacccacgc gcacgttgcc acccatgacg gcgcccatgg
      421 tcagcagcga catctggtgc cggcccgcgc cgaggacgga gaaacggtag ttctcgcggc
      481 cgaacagccg atcggccgtg gtgcgcatca ttaccaggtt ctctggatcg gggccgacgc
      541 cgccgaggat gccgtagatc gactggatga acagtgggcc tttgaccaat ccttcgtcga
      601 tgaagtaagc taggttgtag aggtggccga tgtcgtagca ctcaaactcg aagcgcgtgc
      661 cgcagccatc gcccagggtc tgcaagatgt gcttgatgtc cttgaaagtg ttgcgaaaga
      721 tcaagtcttc catgccctct acatacggtt tctcccaggg gtgcttccaa tccgtgatct
      781 tgtgagcggc ctgatggatg gagaaattca tcgagcccat gttcagcgag cacatctcgg
      841 gcttggccat tagcgggtag gccaagcgtt cctccagcgt catgcgcgtg ctgccgccgc
      901 tggtgatgtt gatgacagcg tccgtcgccg ctttgatgcg cggcacgaat tggtcgaaga
      961 tcttcggatc cggcgtgggg ctgccgtcca ccgggttgcg tgcatggaga tgcaggatcg
     1021 cggcgcccgc ttcggccgcc tcgatcgact gctcagcgat ctgatccggt gtgatgggca
     1081 ggtacggcga catggtcggc gtgtgcatcg cgccggtcac ggcacaactg atgatgactt
     1141 tgtcttgtgg cttggccatg atggtcgcgg tctccagagg ttcgtttggc ggttccatct
     1201 gcctcgatac atgaggccgg acgtcatgcg acgtggccgg atcaatcgct gcaaactggc
     1261 acgaatcgta gaagtcgcat cgcttgtaaa acaggtcgct cccggtctat ggcgggtgca
     1321 taccggacat ctatgggtca gtaaaaaccc aggggtacgc gcctgcgagg acggtgatac
     1381 tgcgcaccgg gcgcttttgc ccctgtcctg gcaactccac cgctggctcc ttatcgatgt
     1441 ctgcaaaacc ctggatcaac gagaaaatct acgcgccgta caagctcgca gcgttggtcg
     1501 agacgctgac cgaacttggc gtaccctcac agatgagctt gcaggggaca ggcttggcgc
     1561 tgtgcgacgt agagaacccg gaaaccaaga cgtcggtcgg tcaatacttc acggcttgcc
     1621 ggaacgccgt gcgcacgtcc aagacgccgg ccgcgccttt cctggttgga cggagaatgc
     1681 acctgtcggc ttatggcatg tatgggtacg cgttgatgtg cagcttgacg ctgcgcgact
     1741 ttttcaacga gggcgtcaag taccacaagc ttgcgacgcc cacagtggtc atcgaatggc
     1801 gtgagcaaga tggcgccgcc atctggagct tcccacggtt ggtggcacca gaactcagcg
     1861 tcgacttgtt tcagttcctg atcgagcagc aactggtgca acacgccatc catttgaacg
     1921 acgtggcagg cccggaatgc cggccgctgc gcgcgctgct gtcgtaccct gcgcctgcgc
     1981 acgagcacct gtacgcggag taccttggtt gcccggcgta cttcggcagc gaaacctgcg
     2041 agctgcatta cgacgctgcc atccttgacc aaccgactca gctggcacac aagctcactt
     2101 cggccctcat gcaggcgacc tgtgagcggt taatcggcca ggccaagacc accgtcggca
     2161 tctcgggtca ggtctaccag atcctgatgg cgagtccaga gcaactgccg cgcatggaag
     2221 atgtggcggc catggtgcac atgaacgagc gcaccatgcg ccgcaagctc gaatctgagg
     2281 gcacctcatt tggtcagatc atcgacgacg tgcgagcgtc gttggccgct gagtacctca
     2341 agaccacgaa gatgacgacg gaggacatcg ctgcactcgt tgggttcagc gatgctgcga
     2401 acttccgccg agccttcaag cgctggacgg gcaagacgcc gagcgaatat cgaacctgac
     2461 gaccttgcct tcccaattca acggttcgat gagccggcgc tcgaatcgac gagcaccact
     2521 atcaacattg ccttctgcgt accgcggttg atccaggcat ggttggtacc acggtgcagg
     2581 atcgcatcga agggtttcaa tgtgtgcttc gtcttgtcga gcacgccccg cgcgggcgcg
     2641 tcagccaggc cagcaccagg cacagcgcca gcgtgggcag ggccgcgccc agcccgggca
     2701 gcacgcgcgg cagcaggtga tagcaggcaa tgccgcccag ccacagcgcc acgggcaggc
     2761 cgtgcacggc gcgcgcatcc agcagccgct gcggcgcggc cacgccgaac gccaggcgtc
     2821 ccaggatcac gccgaacagc ggcacgaaca ccgagctgag cagcagcaaa aacggctcca
     2881 ggctgtgcat gggcagcacc agggccaggc ccgtgcacag cgcggccagc accaggcccc
     2941 agcggcgcac gctccagccc ggccgcaggc tgtgcgccga gacggcgccc gaatacgcgt
     3001 cgccataggc gttgtccacc tcgtcaatga ggatcagcga cagcgccagc agcccgccct
     3061 gggccagcag cagggccgtc accagatcct ggctgggcag ggtcagcgcc accagcacgc
     3121 ccaggcagta gcaccagatg ttggccagcg catagcccag ccagctgccg cgcagggcgc
     3181 cggcgccgct gacgccatgg cgcgcgtagt cggccaccag cggcagccag gagacgggca
     3241 tggcgatcac cagatccagc gccggcatca cccccatgcc gcccgcgccg cggcgctgcc
     3301 agatctcgtc cagcccctgg gcctgggcca tggacaggaa ttgccaggac agccagatca
     3361 gcgacagcac caccagcggc agcgccacgc gcgcaatcac gcggcgcacc agccgcacca
     3421 tggagccgct gatcagaagc gccaccacgc cgccccacag cagcgtggcc gccaccggcc
     3481 accaggcggc ggccagcagg cccgactgcc ggcctatggc cacggtggcg tcgcgcatca
     3541 ccaccagctc gaaggtgccc cagcccacca gctgcacgat gttcagcaat atgggcaggc
     3601 tggcgaagcc gcggccatag acggcgtgca tcagcccggc gctggccagg ccgctgtcgc
     3661 agcccagttt tgccacccag cccagcaggc ctgcgccgat gatggagccg gcgacgatgg
     3721 ccagcagcgc ctgctgcgtg cccaaggctg gcaccagg
//
LOCUS       DAOMTQ010000226         3718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6796_length_3718_cov_27.635545, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000226 DAOMTQ010000000
VERSION     DAOMTQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6796_length_3718_cov_27.635545"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="proV"
                     /locus_tag="PK518_14920"
     CDS             complement(<1..1196)
                     /gene="proV"
                     /locus_tag="PK518_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HRM50251.1"
                     /translation="MAKQIIIDQVFKVFGDAPEQAMDLVRQGLGKQDILARTGHSIGV
                     FDASFTIEPGEIFVVMGLSGSGKSTLVRMLNRLIEPTAGRILVDGQDIHALTDKALRQ
                     WRRKDISMVFQSFALMPHMTVLDNTAFGLELAGTPKAERQQMARQALEQVGLADWGAS
                     YPDELSGGMQQRVGLARALAADPAILLMDEAFSALDPIIRTEMQSELLRLQQLKRRTI
                     VFISHDLDEAMRIGDRIAIMKDGQVVQVGTPDEILRKPANGYVRDFVRGVDAAAVFKA
                     GDIARQAFTVTSAREDRGSRATLRLLEDSDRDHAYVLTSRKQYLGVVSTQSLRDALHG
                     HHGPLGLRHAFLPDIPQLAADTPVAELYGPMGSAPCPLPVVAEDGRFLGVVSRTTLMK
                     FLDRDTP"
     gene            1679..3673
                     /gene="acs"
                     /locus_tag="PK518_14925"
     CDS             1679..3673
                     /gene="acs"
                     /locus_tag="PK518_14925"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRM50252.1"
                     /translation="MSDSSSAIQSVLVENRVFPPPEALVKAARISGMAGYEALCAEAN
                     KDFEGFWARLARENINWTKPFTQTLDESNAPFYKWFADGELNASANCLDKHMGTPVEN
                     KTAIIFEADGGEVTKVTYKELLARVSQFANALKARGVQKGDRVLIYMPMTIEGIVAMQ
                     ACARIGATHSVVFGGFSAKAVQERIQDAGAVAVITANYQLRGGKELPLKAIVDEAIAS
                     GGCECVKTVFVYERTTSAWARVAGRDISFAEAVAGQSTECAPVPVEAEHPLFVLYTSG
                     STGKPKGVQHATGGYMLWAKLTMDWTFDIQPSDVFWCTADIGWVTGHTYITYGPLAAG
                     ATEVVFEGVPTYPNAGRFWQMIERHKVSIFYTAPTAIRSLIKAADSDEAVHPGKSDLS
                     SLRILGSVGEPINPEAWMWYYKNVGQERCPIVDTWWQTENGGHLITPLPGATPLVPGS
                     CTLPLPGIDAAIVDETGNDVPNGSGGILVIKRPWPSMIRTIWGDPERFKKSYFPEELK
                     GYYLAGDGAVRDAKTGYFRITGRIDDVLNVSGHRMGTMEIESALVAKSDLVAEAAVVG
                     RPDDLTGEAICAFVVLKRPRPTGDEAKQIAKELRDWVAKEIGPIAKPKDIRFGDNLPK
                     TRSGKIMRRLLRSLAKGESITQDTSTLENPAILDQLAQNN"
BASE COUNT          669 a         1234 c         1224 g          591 t
ORIGIN      
        1 ggcgtgtcgc ggtccagaaa cttcatcagc gtggtgcggc tgaccacgcc caggaagcgt
       61 ccgtcctcgg ccaccacggg cagcgggcag ggggcgctgc ccatggggcc gtacagctcc
      121 gccaccgggg tgtcggcggc aagctggggg atgtccggca gaaaggcgtg ccgcagcccc
      181 agcggcccgt ggtggccgtg cagggcgtcg cgcagcgact gcgtgctgac gacccccagg
      241 tattgcttgc ggctggtcag cacgtaggcg tggtcgcggt ctgaatcttc cagcaggcgc
      301 agcgtggcgc ggctgccgcg gtcctcgcgc gccgatgtca cggtgaaggc ctggcgcgca
      361 atgtcgccgg ccttgaacac cgccgccgca tccaccccgc gcacgaagtc gcgcacatag
      421 ccgttggccg gcttgcgcaa gatctcgtcg ggcgtaccca cctgcaccac ctggccgtcc
      481 ttcatgatgg cgatgcggtc gccaatgcgc atggcctcgt ccaggtcgtg cgagatgaag
      541 acgatggtgc gccgcttgag ctgctgcagg cgcaggagct cggactgcat ctcggtgcgg
      601 atgatggggt cgagtgccga gaaggcctcg tccatcagca ggatggccgg gtcggccgcc
      661 agcgcgcgcg ccaggcccac gcgctgctgc atgccgccgg agagctcgtc cgggtagctc
      721 gcgccccagt cggccaggcc cacctgctcc agcgcctgcc gcgccatttg ctggcgttcg
      781 gccttgggcg tgccggccag ctccaggccg aaggcggtgt tgtccagcac cgtcatgtgc
      841 ggcatcagcg caaacgactg gaacaccatc gagatgtcct tgcgtcgcca ctggcgcagg
      901 gccttgtcgg tcagcgcatg gatgtcctgg ccatcgacca ggatgcggcc cgccgtgggc
      961 tcgatgagcc ggttgagcat gcgcaccagc gtggatttgc ccgagcccga caggcccatg
     1021 acgacgaaaa tctcgccggg ctcgatcgtg aagctggcgt cgaacacgcc gatcgaatgg
     1081 cccgtgcggg ccagtatgtc ctgtttgccc aggccctggc gtaccagatc catggcctgt
     1141 tcgggcgcgt ccccgaagac cttgaaaacc tggtcgatga tgatttgctt ggccatcatg
     1201 accctcctgt gtagagaaag cgggttgacg ccgtccgacg gcggcctgac gtgctgccag
     1261 gcatgcgccg ccaatggctg cgcctggcac tcaggcggcc gggacgttca acgcaagtga
     1321 ctgaaagcgg ctcgcatggc acctggggtg gtgcgcaaga gcctgaaagg catcacggcc
     1381 gcaacctggc ggccgtggag gcggcaatgg cattgctgct gcaagccgtg ccaacctgcc
     1441 gaaaaaccca ctccaggtca tcagggatag gagcgggcaa cctgcgtggc aggtggtgaa
     1501 acatgtaaaa agtatatgaa tgcagggttt tcgagtcaaa acacaagttt ttcaaagcat
     1561 gctattcgcg cgagcgctgc tttttgcctg gtttgcagcc gtctgccgcg tcagtggctt
     1621 gtaagtagca ggcacgaaag tacgcccgga ttttttcagt cacttggaga caacccacat
     1681 gagcgattcc tcttccgcca tccagtctgt tttggttgaa aaccgtgtct tccccccgcc
     1741 agaggcgctg gtgaaggccg cccgcatctc cggcatggcc ggctacgaag ccctgtgcgc
     1801 cgaggcgaac aaggatttcg agggcttctg ggcccgtctg gcgcgcgaga acatcaactg
     1861 gaccaagcct ttcacgcaaa ccctggacga gtcgaatgca ccgttctaca agtggtttgc
     1921 cgacggcgag ctgaacgcca gcgccaactg cctggacaag cacatgggga cgccggtcga
     1981 gaacaagacc gccatcatct tcgaggccga cggcggtgag gtcaccaagg tcacctacaa
     2041 ggagctgctg gcgcgcgtga gccagtttgc caacgccctg aaggcgcgcg gcgtgcaaaa
     2101 gggcgaccgc gtgctgatct acatgcccat gaccatcgag ggcatcgtgg ccatgcaggc
     2161 ctgcgcgcgc atcggcgcca cgcacagcgt ggtgttcggc ggcttctcgg ccaaggctgt
     2221 gcaggagcgc atccaggatg ccggcgccgt ggccgtgatc accgccaact accagctgcg
     2281 cggcggcaag gagctgccgc tgaaggccat cgtggatgag gccattgcct cgggcggctg
     2341 cgagtgcgtg aagaccgtgt tcgtgtacga gcgcaccacc agcgcctggg cccgcgtggc
     2401 cggccgcgac atctcgtttg ccgaggccgt cgccggccag agcaccgaat gcgcccccgt
     2461 gccggtggaa gccgagcacc cgctgttcgt gctctacacc agcggctcca cgggcaagcc
     2521 caagggcgtg cagcatgcca ccggcggcta catgctgtgg gccaagctga ccatggactg
     2581 gaccttcgac atccagccca gcgacgtgtt ctggtgcacg gccgacatcg gctgggtcac
     2641 gggccacacc tacatcacct acgggccgct cgccgccggc gccaccgagg tggtgttcga
     2701 gggcgtgccc acctacccca acgccggtcg cttctggcag atgatcgagc gccacaaggt
     2761 cagcatcttc tacaccgcgc ccacggccat tcgctcgctg atcaaggcgg ccgattcgga
     2821 cgaggcagtg catccgggca agtcggatct gtcgagcctg cgcatcctgg gcagcgtggg
     2881 cgagcccatc aaccccgaag cctggatgtg gtactacaag aacgtgggcc aggagcgctg
     2941 ccccatcgtg gacacctggt ggcagacgga aaacggcggc cacctcatca ccccgctgcc
     3001 gggcgccacg ccgctggtgc cgggcagctg cacgctgccg ctgccgggca tcgatgccgc
     3061 catcgtcgat gagacgggca acgacgtgcc caacggctcg ggcggcatcc tggtcatcaa
     3121 gcgcccctgg ccatcgatga tccgcaccat ctggggcgac cccgagcgct tcaagaagag
     3181 ctacttcccc gaagagctca agggctacta cctggccggc gacggcgccg tgcgtgacgc
     3241 caagaccggc tacttccgca ttaccggccg catcgatgac gtgctcaacg tctcgggcca
     3301 ccgcatgggc acgatggaaa tcgaatccgc cctggtggcc aagagcgacc tggtggccga
     3361 ggccgccgtg gtgggccgcc ccgacgacct gacgggcgag gccatctgcg ccttcgtggt
     3421 gctgaagcgc ccgcgcccca ccggcgacga ggccaagcaa atcgccaagg agctgcgcga
     3481 ctgggtggcc aaggaaatcg gccccatcgc gaaacccaag gacatccgct tcggcgacaa
     3541 cctgcccaag acccgcagcg gcaagatcat gcgccgcctg ctgcgatcgc tggccaaggg
     3601 cgagtccatc acccaggaca ccagcacgct ggaaaacccc gcaattctcg atcagttggc
     3661 gcagaacaac tgatcgagaa ggcagcgcaa gctgccagcg cgtagcgctt gcggggtt
//
LOCUS       DAOMTQ010000227         3717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6797_length_3717_cov_25.490169, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000227 DAOMTQ010000000
VERSION     DAOMTQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_3717_cov_25.490169"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK518_14930"
     CDS             complement(<1..211)
                     /locus_tag="PK518_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM50253.1"
                     /translation="MFADSRLSPLPHVPARAPAMPPALRVGLIGMGTVGTGVWRVLRR
                     NQALIAARAGRAVEIVAVATRTPARA"
     gene            594..896
                     /locus_tag="PK518_14935"
     CDS             594..896
                     /locus_tag="PK518_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRM50254.1"
                     /translation="MTAAPTPTITRLLRIHGQVQGVYYRQSMIEAASRLGVRGWVRNR
                     LDGTVEALASGPAEAVQALIAWAHDGPPAARVQRVQVEEMAIDEESAPGFHRRETV"
     gene            complement(897..1142)
                     /locus_tag="PK518_14940"
     CDS             complement(897..1142)
                     /locus_tag="PK518_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM50255.1"
                     /translation="MHTARKALPAIDPGRCTGCGWCVAVCAPHVLSLQVQGESGWGPK
                     RSTLHDADGCTGCALCALRCPFDAIRMVRVSKPKAGA"
     gene            complement(1149..1772)
                     /locus_tag="PK518_14945"
     CDS             complement(1149..1772)
                     /locus_tag="PK518_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRM50256.1"
                     /translation="MYIPEHFAESRLDELHRIMRAHPLAMLCTSGPQGLDANHLPFLL
                     DADSGPHGRLIGHVARANPLWQSVAPDSEVLVVFRGAEGYVSPNWYPSKHETHRAVPT
                     WNYEVVHAHGTLTIHDDVKHVRSVVARLTHAHEAGEPHPWKMGDAPPDYLADMLRAIV
                     GIEVHITHLEGKRKLSQNRGTADRSGVMAALHERDHRALAQAMQQTG"
     gene            complement(1837..2235)
                     /locus_tag="PK518_14950"
     CDS             complement(1837..2235)
                     /locus_tag="PK518_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRM50257.1"
                     /translation="MRSPIAIVDVDRPDSWTRYRAGLCASCAANCCTMPLEVQLSDLV
                     RLGLVDAFEAEHEDPKRIAKRLQKARLIDHFNHKHLLFTLARRADGDCQYLDARTRLC
                     TVYEQRPETCRLHPQKKSPKPGWCPYGRKA"
     gene            complement(2232..2732)
                     /locus_tag="PK518_14955"
     CDS             complement(2232..2732)
                     /locus_tag="PK518_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM50258.1"
                     /translation="MPPLTIRAATPADIPVILRFVRALAVYENAEHEVLATPDHVQRT
                     LFCNQPKVFGLICEQGDTPVGFAVYFFNYSTWQGRHGLYLEDLYVAPEHRGKGAGMAL
                     LQHLAQIAVAQDCGRFEWSVLDWNTPSIALYDSLGARPQSEWIRYRLTGAELQALADG
                     ARPQTP"
     gene            complement(2803..>3717)
                     /locus_tag="PK518_14960"
     CDS             complement(2803..>3717)
                     /locus_tag="PK518_14960"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HRM50259.1"
                     /translation="VPTHWLLGRPSEAIAAMARHAGGAGRAAQADYQALQAVLGAVDG
                     QFHPLLLRRLALWRAAPVDEVITAARMALQLAPGCAQGRPLRALAVAGNDSKFFERHA
                     ALLTALLDLRFDGAAGRAGLSAFLDASADDEHWLLVAPLAPGLLPFARQRVPASELQA
                     TPLPAAHILLVENERCLHLLPRPLAGTIAILGAGRNLAWLAAPWLQARRVAYWGDIDT
                     WGLAMLAGARGHLPHLQALLMDRATLEAHPDRAVAEPQPAPEPGPGALLAQELALDAH
                     LRGLARGRLEQEFLNPARVAQALTGWAA"
BASE COUNT          546 a         1237 c         1376 g          558 t
ORIGIN      
        1 ccgcccgcgc cggggtgcgc gtggccacgg cgacgatctc cacggcccgc ccggcgcgcg
       61 ccgcgatcag tgcctggttg cgccgcaaca cgcgccagac cccggtgccc acggtgccca
      121 tgccaatcag gcccacgcgc agggcagggg gcatggccgg cgcgcgcgcg ggcacatggg
      181 gcaaggggct gagccgggaa tctgcaaaca tggtcaatct ccaaacaagg ctgcgagagc
      241 caaaacaaaa aaacccagcg gccgggctgg gtttcgttcg gagggggaat gagacaccag
      301 ggggccgcct tagcggccac cctgcgcgtg cgctaggcag ccgcggtggt catggtgcta
      361 atcatgcgtg cgctcgcgcc ggccggcgcc aaatgggcgc tggtgctggt gcgtgcgagg
      421 tgcaatgaca tggggcgcag tgtagcgcag cgcgtgatta cgctcttgtt gcttttatat
      481 tgatagctga tggcgcttta tccataagcg ctacaagcca ttttggttgt agtgacatgg
      541 cgtgtgcgac atggtggccg gcaagccgat gggcagggcg ctacagtccg tgcatgaccg
      601 ctgcacccac ccccaccatc acccgcctgc tgcgcatcca tggccaggtg cagggggtgt
      661 attaccgtca atccatgatc gaggccgcct ctcgcctggg tgtgcggggc tgggtgcgca
      721 accgcctgga tggcacggtg gaggccctgg ccagcgggcc ggcagaggcc gtgcaggcac
      781 tcatcgcctg ggcgcacgac ggcccgccag cggcgcgcgt gcagcgggtg caggtcgagg
      841 aaatggccat cgatgaagag tccgcgcccg ggttccatcg gcgcgagacg gtctgatcaa
      901 gcgcccgcct tgggcttgct cacgcgcacc atgcgaatcg cgtcgaacgg gcagcgcagc
      961 gcgcacagcg cgcagccggt gcagccatcg gcatcgtgca gcgtggagcg cttgggcccc
     1021 cagccggatt cgccctgcac ctgcagcgac agcacatgcg gcgcgcacac cgccacgcac
     1081 cagccgcagc cggtgcagcg gccggggtct atggcgggta gggccttgcg tgctgtgtgc
     1141 atgctgtact agccggtctg ctgcatggcc tgggccaacg cccggtgatc gcgttcgtgc
     1201 agcgcggcca tgacgccgct gcgatccgcc gtgccgcggt tctggctcag cttgcgcttg
     1261 ccctccaggt gcgtgatgtg tacctcgatg ccgacgatgg cgcgcagcat gtcggccagg
     1321 taatcgggcg gcgcgtcgcc catcttccag ggatgcggtt cgccggcctc gtgcgcatgc
     1381 gtcaggcgcg ccaccaccga gcgcacatgc ttcacgtcgt catggatggt gagcgtgccg
     1441 tgggcgtgca cgacctcgta attccaggtg ggcacggcgc ggtgcgtctc gtgtttgctc
     1501 gggtaccagt tgggcgagac atagccctcg gcgccgcgaa acaccaccag cacctcgctg
     1561 tcgggcgcca cgctctgcca cagcggattg gcgcgcgcca catggcctat caggcggccg
     1621 tgcgggccgc tgtcggcgtc gagcagaaag ggcaggtggt tggcgtccag cccctgcggg
     1681 cccgacgtgc acagcatggc cagcggatgg gcgcgcatga tgcggtgcaa ctcgtccagg
     1741 cgtgattcgg cgaagtgttc tgggatgtac atggcgggcc cgttcaggtg gtggccctat
     1801 ggtacggccg cccccgcggc agggttgccc ctgccgctat gccttgcggc cgtaggggca
     1861 ccagcccggc ttggggcttt tcttctgcgg gtgcaggcgg caggtctctg ggcgctgctc
     1921 gtacaccgtg cacagccggg tcctggcgtc caggtactgg cagtcgccat cggcgcgccg
     1981 cgccagcgtg aacagcaggt gcttgtggtt gaagtggtct atcagccgcg ccttctgcag
     2041 ccgcttggcg atgcgtttgg ggtcctcgtg ctcggcttcg aaggcgtcca ccaggcccag
     2101 gcgcaccagg tcggagagct gcacctccag cggcatggtg cagcagttgg cggcgcagct
     2161 ggcgcacagg ccggcgcggt agcgcgtcca gctgtccggg cggtccacgt caacgatggc
     2221 gatgggcgat ctcatggcgt ttggggccgg gcgccatcgg ccagcgcctg cagctcggcg
     2281 cccgtcaggc ggtagcgtat ccactcgctc tgcggccggg cgcccaggct gtcgtagagg
     2341 gcgatggagg gcgtgttcca gtccagtacg ctccattcga agcgaccgca gtcctgggcc
     2401 acggcgatct gcgccaggtg ctgtaagagc gccatgcctg cgcccttgcc gcggtgctcg
     2461 ggcgcgacgt acagatcctc caggtacaac ccgtggcggc cctgccaggt ggaatagttg
     2521 aagaagtaga cggcaaagcc cacgggcgta tcaccctgtt cgcagatcaa accgaacacc
     2581 ttgggctggt tgcagaacag cgtgcgctgc acatgatcgg gcgtggccag cacctcgtgt
     2641 tcggcgttct cgtacacggc cagcgcgcgc acgaagcgca ggatgacggg gatgtcggcc
     2701 ggggtggccg ctcggatggt caaggggggc atgggaggcg gaaaggaagg cggcggcgcg
     2761 cagcgctgca cggtgggccg ctgggccgtt aggggcgtga ttttaggcag cccagcccgt
     2821 cagcgcctgc gccacgcggg cggggttcag aaactcctgc tccaagcggc cacgcgccag
     2881 gccgcgcaga tgtgcgtcca gcgccagctc ctgtgccagc agggcgccgg ggccgggttc
     2941 tggtgccggc tgcggctcgg ccacggcgcg gtcagggtgc gcctccagcg tggcccggtc
     3001 catcagcagc gcctgcagat gcggcagatg gccgcgcgcg ccggccagca tggccaggcc
     3061 ccaggtgtct atgtcgcccc aataggccac gcggcgcgcc tgcagccagg gcgcggccag
     3121 ccaggccagg ttgcgccccg cgcccaggat ggcgatggtg cccgccagcg gccggggcag
     3181 caggtgcagg cagcgttcgt tctccaccag caggatgtgg gcggcgggca ggggcgtggc
     3241 ctgcagctcg ctggccggca cgcgctggcg cgcgaagggc agcaggccgg gggccagggg
     3301 cgcgaccagc agccaatgct cgtcgtcggc gctggcatcc agaaaggcgc tcaggcccgc
     3361 gcggccggcg gcgccgtcaa agcgcaggtc gagcagggcg gtgagcaggg ccgcgtggcg
     3421 ctcgaaaaac ttgctgtcgt tgcccgccac ggccagggcg cgcagcggcc ggccctgggc
     3481 gcagccgggc gcgagctgca gcgccatgcg cgcggcggtg atcacctcat ctaccggagc
     3541 cgcgcgccac agcgccaggc ggcgcagcag cagcgggtga aactgcccat cgaccgcacc
     3601 cagcacggct tgcagcgcct ggtagtcggc ctgggccgcc cggcccgcgc cgcccgcatg
     3661 ccgggccatg gcggcgatcg cctcggacgg ccggcccagc agccaatgcg tgggcac
//
LOCUS       DAOMTQ010000228         3701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6850_length_3701_cov_20.121777, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000228 DAOMTQ010000000
VERSION     DAOMTQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_3701_cov_20.121777"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1626
                     /locus_tag="PK518_14965"
     CDS             100..1626
                     /locus_tag="PK518_14965"
                     /inference="COORDINATES: protein motif:HMM:NF039478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HRM50260.1"
                     /translation="MGAGQSWRIAATGAGAPATLPAASLLISAGCYYVQGQHCVWEQA
                     ASLANQPDGGVALRLAPGQHLIYLRAWQRHISALEDPAIRELALGGPDTATRTRQMAQ
                     VRALALPTPANNQAWHCGAPVAGWDSLTAAPGARLAARAEPQLAATSVCDIAASAGYR
                     RLENQLYRVEIHSVDANTGAATFKWSRENASVAYAVQSVTIDSVAQQTIVRVAARGRD
                     ANLDLSPHDRVELLDDASAREQRAGQLFEFLHDGDDALELVLGGLPQSSLGQDASGHP
                     LLRRWEQRPTAAGQHALAVVEGGWIPLEDGVQVRFAPGGSYRVGDYWQIPARTATADV
                     EWPLDEDGAPQACPPAGVRDGWARLGLVTVDAGGLISAITDCREWFPPLTQLTQLLYV
                     GGDGQEAAPNTTLAEPLRLRVARGALPLAGSRVRFTLEAGGGSVNPPRPRPMRRAWHS
                     ATGSWAAPGRSACAPTCSPAAAQPCPSRHWCLPPWPRHRRRRAAVVASPLARAATLSS
                     "
     gene            1521..2540
                     /locus_tag="PK518_14970"
     CDS             1521..2540
                     /locus_tag="PK518_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50261.1"
                     /translation="MPEQTLVFAAMAQAPAQAGGGCCITIGKGGDFEQLSTELLMKLL
                     EEHGGQLCLCLLPGTHELADLTVNQVPPGARLSLHGCGPTAIVAVHAPIALGGFAALE
                     LRDLQLAMDPGTSLQLTGNRELGLHSLRVRGKQDQPQPWIVVDGSSELRMHQCEVAAA
                     APASVVLQNITGQCEVMHCVIAGDLAFYGMPPPDGTASAPIATVLVNALISGDFQVPA
                     GRGQLVLGHNRLRRLNLGEEMLKQLIQRQFNGLFQTVVLQGNTFSSAPSLCAGVLLTV
                     NGNHFTAGQTAENLHGVLAGRRAAASGNASDLLNDKMTLVFLLTNRTQFRGAANLMPT
                     TPPSI"
     gene            complement(2583..>3701)
                     /locus_tag="PK518_14975"
     CDS             complement(2583..>3701)
                     /locus_tag="PK518_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRM50262.1"
                     /translation="GLLPSLGASGAAIRARGAGSGSGAGTGNSYSLGLNASWELDLWG
                     RLSGAVDASQAQAQASSADLAAARLSLQASLAQAYFALRTAEAQQRLLQESLAAYEQS
                     WQLTRNRERAGVASSADVAQAEAQYKSTQAQLIEAGTSRAQLEHALAALLGLAPAAFD
                     LPATGLLPAPPLVPAELPAELLRRRPDIAAAERRVAAANADLGVARTAYFPALTLSGA
                     ADYRGARLSDLVSAPQLFWSLGPALAATLFDGGARDASVESARAALDLATAGYRQSVL
                     TALQEVEDNLAAATSLAQQQQVQTEAVAAAQRALDVVSNQYRAGTVAYLNVLSAQTTV
                     LAARRSLIDVQGRRLVAVNTLLKNVAGRWESAESYQKQ"
BASE COUNT          533 a         1340 c         1332 g          496 t
ORIGIN      
        1 cgggtgagca gccggtggcc gaggaccggg ccggccagcc gcttcacgtc gtccgggacg
       61 gtgtagtccc ggccgtccag cacggccgac gcctgggccg tgggcgccgg ccagtcctgg
      121 cgcatcgcgg ccacgggcgc gggcgccccc gccaccctgc ccgcggccag cctgctgatc
      181 agcgccggct gctactacgt gcagggccag cattgcgtct gggagcaggc cgcatcgctg
      241 gccaaccagc ccgatggcgg cgtggccctg cgcctggcgc cgggccagca cctgatctac
      301 ctgcgcgcct ggcagcgcca catcagcgcg ttggaagacc ccgccatccg cgagctggcc
      361 ctgggcgggc cggacacggc cacacgcacg cgccagatgg cccaggtacg cgccctggcc
      421 ctgccgaccc cggccaacaa ccaagcctgg cactgcggcg cgccagtggc tggctgggac
      481 agcctgacgg cagcgcccgg cgcccgcctg gcggcacgcg ccgagccgca gctggcggcc
      541 accagcgtgt gcgacatcgc agccagcgcc ggctaccggc ggctggaaaa ccagctctac
      601 cgcgtggaga tccacagcgt ggacgccaac accggcgccg ccaccttcaa gtggtcgcgc
      661 gaaaacgcct cggtggccta cgccgtgcag tccgtgacca tcgacagcgt ggcccagcag
      721 accatcgtgc gcgtggccgc gcgcgggcgc gatgccaacc tggatctgtc gccgcacgac
      781 cgcgtggagc tgctcgacga tgcgagcgcg cgggagcaac gcgccggcca gctgttcgag
      841 tttctgcacg acggcgacga cgcgctggag ctggtgctcg gcggtctgcc ccagagcagc
      901 ctgggccagg acgccagcgg ccacccgctg ctgcggcgct gggagcagcg ccccactgcc
      961 gccggccagc acgcgctggc cgtggtcgag ggcggctgga ttcccctgga agacggcgtg
     1021 caggtgcgct ttgcccccgg cggcagctac cgcgtgggcg actactggca gatccccgcg
     1081 cgcacggcca cggccgatgt cgagtggccg ctggacgagg acggggcgcc gcaagcctgc
     1141 ccgcccgccg gtgtgcgtga cggctgggcg cgcctgggcc tggtgacggt ggacgcgggt
     1201 ggcctgatca gcgccatcac cgattgccgc gaatggttcc cgccactcac ccagctgacg
     1261 cagctgctct acgtgggcgg cgacggccag gaggccgcgc ccaacaccac cctggccgaa
     1321 ccgctgcgcc tgcgcgtggc acgcggcgcg ctgcccctgg ccggcagccg ggtgcgcttc
     1381 acgctggagg ccggtggcgg cagcgtcaac cccccgaggc cacgaccgat gcgcagggcc
     1441 tggcacagtg caactggcag ctgggcggcg ccgggccgca gcgcgtgcgc gccaacctgc
     1501 tcaccagcgg cggcgcagcc gtgcccgagc agacactggt gtttgccgcc atggcccagg
     1561 caccggcgca ggcgggcggc ggttgttgca tcaccattgg caagggcggc gactttgagc
     1621 agctgagcac cgagctgctg atgaaactgc tggaggaaca tggcggccag ctgtgcctat
     1681 gcctgctgcc cggcacgcat gagctcgcag acctgacggt gaaccaggtg ccgcccggag
     1741 ccaggctgtc gctgcacggc tgtggcccca cggccatcgt cgccgtgcac gcacccatcg
     1801 cgctgggcgg gtttgccgcg ctggagctgc gcgacctgca gctggccatg gaccccggca
     1861 cctcgctgca actgacgggc aaccgcgaac tgggcctgca cagcctgcgc gtgcggggaa
     1921 agcaggatca gccccagccc tggatcgtgg tggacggcag cagcgagctg cgcatgcacc
     1981 agtgcgaggt ggcggcggcg gcgccggcct cggtggtgct gcagaacatc accgggcaat
     2041 gcgaggtcat gcactgcgtg atcgctggcg acctggcgtt ctatggcatg ccgccgcccg
     2101 acggcacggc ttcggcacct atcgcaacgg ttctggtgaa tgcgttgatc agcggcgact
     2161 tccaggtgcc ggccggccgc ggccagctgg tgctggggca caaccggttg cgccgcctga
     2221 acctgggcga ggaaatgctc aagcagttga tacaacggca attcaacggc ctgttccaga
     2281 ccgtggtgct gcagggcaat accttcagca gcgcgcccag cctgtgcgcc ggcgtgctcc
     2341 tgacggtcaa cggcaaccat ttcacagccg gccagacagc ggaaaacctg catggcgtgc
     2401 tggcgggccg ccgcgcggcc gccagcggca atgcaagcga cctgctcaat gacaagatga
     2461 cgctggtgtt cctgctcacc aaccgcaccc agttccgtgg cgcggccaac ctgatgccca
     2521 cgacgccgcc atcgatttga agaaaaattc gaccgtagcg cctgaccgtc aagcgctgac
     2581 agctattgtt tttgatagct ttcggcgctc tcccagcgcc cggccacatt tttcagcagc
     2641 gtattcaccg ccaccaggcg gcggccctgc acgtcgatca ggctgcgccg ggcggccagc
     2701 accgtggtct gggccgagag cacgttgagg taggccacgg tgcccgcgcg atactggttg
     2761 ctcactacat ccagcgccct ttgcgcggcg gcgacggcct cggtctgtac ctgctgctgc
     2821 tgggccaggc tggtggcggc ggccaggttg tcctcgacct cctgcagcgc cgtgagcacg
     2881 ctctggcggt agccggccgt ggccagatcg agcgcggcgc gggccgactc gaccgacgca
     2941 tcgcgtgccc caccgtcaaa caaggtggcg gccagggccg ggcccagcga ccagaacagc
     3001 tgcggcgcac tcaccagatc tgacaggcgc gcgccacggt agtccgccgc gcccgacagg
     3061 gtgagtgccg gaaagtaggc cgtccgggcc acgcccagat cggcgttggc ggcggccacg
     3121 cggcgctcgg cggcggcgat gtcggggcgc cggcgcagca gctcggccgg cagctcggcg
     3181 ggcaccaggg gaggcgccgg cagcaggccg gtggccggca gatcgaaggc cgcgggcgcc
     3241 aggcccagca aggcggccag ggcgtgctcc agctgggcgc ggctggtgcc ggcctcaatc
     3301 agctgggcct gggtgctttt gtactgggcc tcggcctggg ccacgtcggc gctggaggcc
     3361 acgcctgcgc gctcacggtt gcgcgtgagc tgccagcttt gctcgtaggc ggccaggctc
     3421 tcctgcagca gccgctgctg ggcctcggcg gtgcgcagcg cgaaataggc ttgcgccagg
     3481 ctggcctgca gcgacaggcg cgccgccgcc aggtcggcgc tgctggcctg ggcctgcgcc
     3541 tggctggcat ccaccgcgcc cgacaggcgg ccccatagat ccagctccca gctggcgttc
     3601 aggcccaggc tgtagctgtt gccggtgccc gcaccgctgc ccgagcccgc gccgcgtgcc
     3661 cgtatggccg cgccgctggc gcccaggctg ggcagcaggc c
//
LOCUS       DAOMTQ010000229         3657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_6982_length_3657_cov_27.988617, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000229 DAOMTQ010000000
VERSION     DAOMTQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6982_length_3657_cov_27.988617"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PK518_14980"
     CDS             complement(<1..144)
                     /locus_tag="PK518_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM50263.1"
                     /translation="MAATHYRFKPWNVGRFIVWTLFALLLIAAPQLFASSLALTMLSQ
                     MGYL"
     gene            complement(150..1103)
                     /locus_tag="PK518_14985"
     CDS             complement(150..1103)
                     /locus_tag="PK518_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRM50264.1"
                     /translation="MNLEFFVISLLNGVSYGLLLFMLSSGLTLIFSMMGVLNFAHTSF
                     YMLGAYFAYTLSGTIGFWPALIVAPLAVGLLGAAFERYCLRRVHKFGHVPELLVTFGL
                     SYLVLELVQLVWGRSTVPYGLPPLLQGPLFTLYGTQFPRSRSFIMLVALLMLLAVWLL
                     LTRTRIGLVIQAALKHPQMVEALGHNVPRVFMLVFGGGCALAGLAGVIGGNTYITEPA
                     MAASVGSIIFVVVVVGGMGSLAGAFLASLLIGILQTFAVALDYSLAGLLAHFGMPVGE
                     GSPGWPVLKLTVSQVAPILPYLLLVLILILRPKGLLGTRED"
     gene            complement(1192..2427)
                     /locus_tag="PK518_14990"
     CDS             complement(1192..2427)
                     /locus_tag="PK518_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM50265.1"
                     /translation="MRFAVKTIAACAMLASAGAVFAQKGETVKIAWLDPLSGLMAALG
                     TNQMKSWQYLAEEFSKNNAAGVKFEIVPIDNKLSPQETTSALRSAMDQGVRYVVQGNG
                     SGPALAIIDALDKHNARNKGKEVVYLNYAAVDPDLTNSKCSYWHFRLDADTSMKMEAL
                     TTYMKDLPEVKKVYLINQNYSHGQQVSKYAKEMLKRKRADVEIVGDDLAPLAQVRDFA
                     PYIAKIKQSGADSVITGNWGSDLALLIKAANDAGLNNVKFYTYYAVATGAPTALGAAA
                     AGKVYMVGYSHPNHEGPINPIVRGFKAKFNDDMYTASVYHGFAMLTEAFQRAKSTDPV
                     KVAAVMEGMKFKSFNGEVEMRKADHQLQQGLYITRWEKADAKHPLDAENTGHTFVPVK
                     YYEPYVASTPTSCQMKRPS"
     gene            complement(2563..>3657)
                     /locus_tag="PK518_14995"
     CDS             complement(2563..>3657)
                     /locus_tag="PK518_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM50266.1"
                     /translation="SMCYTSGTTGNPKAALYSHRSTTLHAYAAALPDVMCLSARDSVL
                     PVVPMFHVNAWGIPYSAALTGCKLVFPGPALDGKSVYELMEAEGVTFAAGVPTVWQML
                     LTHVKTNGLQFSSLKRTVIGGSACPPAMITAFQEGYGVSVMHAWGMTEMSPLGTLCSL
                     KNKHLHLPHDEQMHILEKQGRAVYGVEMKIVGADGSEQPWDGKTYGDLLVRGPWILDS
                     YYKGECPLVKDADGVGWFPTGDVATIDADGYMQITDRSKDVIKSGGEWISSIDIENIA
                     MAHPAVAMAACVGMPHPKWDERPIVVVVRRPGQELTREELLAFYEGKTAKWQIPDDVV
                     FVDAIPLGATGKMLKTRLREQLADYKLPGL"
BASE COUNT          654 a         1173 c         1175 g          655 t
ORIGIN      
        1 cagatagccc atctgcgaca gcatggtcag tgccaggctg ctggcaaaca gctgcggcgc
       61 ggcaatcagc agcagcgcaa acagcgtcca gacgatgaag cgccccacgt tccagggctt
      121 gaagcggtaa tgggtggcgg ccatgggggt caatcctcgc gcgttcccag caggcccttg
      181 gggcgcagga tgagtatcaa aaccaatagc aggtagggca atataggagc cacctgcgag
      241 acggttagct tcaaaaccgg ccagcccggg ctgccctccc ccaccggcat gccgaaatgc
      301 gccagcagcc cggccagcga atagtccagc gccacggcaa aggtctgcag gatgccgatc
      361 aagagcgagg ccaggaatgc cccggccagc gagcccatgc cgcccaccac caccaccacg
      421 aagatgatgg agcccaccga cgcggccatg gccggctcgg tgatataggt gttgccgcct
      481 atcaccccgg ccaggccggc aagcgcgcag ccgccgccga acaccagcat gaacacgcgc
      541 ggcacgttgt ggcccagggc ctcgaccatc tgcgggtgct tgagcgccgc ctgtatcacc
      601 aggccgatgc gcgtgcgcgt cagcagcagc cacacggcca gcagcatcag cagggccacc
      661 agcatgatga aggagcgcga ccgggggaac tgcgtgccgt acagcgtgaa cagcggcccc
      721 tgtagcagcg gcggcaggcc atagggcacg gtggagcggc cccagaccag ctgcaccagc
      781 tcgagcacca ggtaggacag gccgaaggtg acgagcaact cgggcacatg gccgaacttg
      841 tgcacccggc gcaggcagta gcgctcgaac gcggcaccca gcaagcccac ggccagcggc
      901 gccacgataa gcgccggcca gaagccgatg gtgccggaca aggtgtaggc gaaatacgcg
      961 cccagcatgt agaagctggt gtgggcgaag ttgagcacgc ccatcatgct gaagatcagc
     1021 gtcagcccgg aactcagcat gaacagcagc agcccatagc tgacgccgtt gagcagcgag
     1081 atcacgaaaa actcaagatt catctaaagc gcctggtggc tgccgaaaaa agggcccgag
     1141 ctcactcggg ccccaagggt tgccccgcct ggagcaatgc cacggtcagg gtcagctggg
     1201 ccgcttcatc tggcaggagg tcggcgtgct ggccacatag ggttcgtaat acttcaccgg
     1261 cacaaaggtg tggccggtgt tctccgcgtc cagcgggtgc ttggcatcgg ccttttccca
     1321 gcgcgtgatg tacaggccct gctgcagctg gtggtcggcc ttgcgcatct ctacctcgcc
     1381 gttgaagctc ttgaacttca tgccctccat gacggccgcc accttcaccg ggtcggtgct
     1441 cttggcgcgc tggaaggcct cggtcagcat ggcaaagccg tggtagaccg aggcggtata
     1501 catgtcgtca ttgaacttgg ccttgaagcc acggacgatg gggttgatcg gcccctcgtg
     1561 gttcgggtgg ctgtagccca ccatatagac cttgccggca gccgccgcac ccaaggccgt
     1621 gggcgcgccc gtggccacgg cgtagtaggt gtagaacttg acgttgttca aacccgcgtc
     1681 gttcgccgcc ttgatcagca gggccagatc ggagccccag ttgccggtga tcacgctgtc
     1741 ggcgcccgac tgcttgatct tggcgatgta gggtgcaaag tcgcgcacct gggccagcgg
     1801 cgccaggtcg tcgccgacga tctccacatc ggcgcgcttg cgtttgagca tctccttggc
     1861 gtacttgctg acctgctgac cgtgcgagta gttctggttg atcaggtaga cctttttgac
     1921 ttcgggcagg tccttcatgt aggtggtcag cgcctccatc ttcatggagg tgtcggcatc
     1981 caggcggaaa tgccagtagc tgcacttgct gttggtcagg tcgggatcga cggcggcgta
     2041 gttgaggtag accacctcct tgcccttgtt gcgcgcgttg tgcttgtcca gcgcatcgat
     2101 gatggccagc gccgggcccg agccattgcc ctgcaccacg tagcgcacgc cctggtccat
     2161 ggccgagcgc agcgcgctgg tggtttcctg cgggctgagc ttgttgtcta tggggacgat
     2221 ctcgaacttc acgccggcgg cgttgttctt gctgaactcc tcggccaggt actgccaact
     2281 cttcatctgg ttcgtgccca gcgccgccat caggcccgac agcggatcca gccaggcgat
     2341 cttgacggtt tcaccctttt gggcaaatac ggccccagcg cttgccagca ttgcgcaagc
     2401 ggctattgtt tttactgcaa acctcatcgt tgtctccttc ctttgcatga atgggcgaca
     2461 gcgtacaggc tgcaacaaat ggcttcttca gggatagccc gtagcagcta tcgccaatgt
     2521 gaccggtgcg ccgcccggcg tgcggcgcac cggcgggcag gctcacagcc cgggcagttt
     2581 gtagtcggcc aattgctcgc gcaggcgggt cttgagcatc ttgccggtgg cgcccagcgg
     2641 gatggcatcg acgaacacca cgtcatcggg tatctgccat ttggccgtct tgccctcgta
     2701 gaaggcaagc agctcctcgc gcgtgagctc ctgccccggg cgcctgacca cgacgacgat
     2761 gggccgctcg tcccacttgg ggtgcggcat gcccacgcag gcggccatgg ccacggccgg
     2821 gtgggccatg gcgatgtttt cgatgtcgat ggagctgatc cactcgccgc ccgacttgat
     2881 cacgtccttg ctgcggtcgg tgatttgcat gtagccgtcg gcgtctatgg tggccacgtc
     2941 gccggtgggg aaccagccca cgccgtccgc gtccttgacc agcgggcatt cgcccttgta
     3001 gtagctgtcc agaatccagg ggccgcgcac cagcaggtcg ccataggtct tgccgtccca
     3061 gggctgctcg ctgccgtcgg cgccgacgat cttcatctcc acgccataga ccgcccgacc
     3121 ctgcttttca agtatgtgca tctgctcgtc atggggcagg tgcaggtgct tgttcttgag
     3181 cgagcacagc gtgcccagcg ggctcatctc ggtcatgccc caggcatgca tcaccgacac
     3241 gccgtagcct tcctggaagg ccgtgatcat ggccggcggg caggccgaac cgccgatcac
     3301 ggtgcgtttg agactgctga actgcagccc gttggtcttc acatgggtca gcagcatctg
     3361 ccacaccgtg ggcacgccgg cagcaaaggt cacgccctcg gcctccatca gctcatacac
     3421 cgacttgcca tcgagcgccg ggccggggaa caccagcttg cagccggtga gcgccgccga
     3481 gtatgggatg ccccaggcgt tgacgtggaa catgggcacc acgggcagca cgctgtcgcg
     3541 cgccgacagg cacatcacat cgggcagggc tgccgcatag gcgtgcagcg tggtggagcg
     3601 gtggctgtac agggcggcct tggggttgcc cgtggtgccg ctggtgtagc acatgct
//
LOCUS       DAOMTQ010000230         3591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7196_length_3591_cov_29.263857, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000230 DAOMTQ010000000
VERSION     DAOMTQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7196_length_3591_cov_29.263857"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /gene="flgK"
                     /locus_tag="PK518_15000"
     CDS             complement(<1..1359)
                     /gene="flgK"
                     /locus_tag="PK518_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRM50267.1"
                     /translation="MSLLSVGARALLANQTALQTTGHNIANVSTAGYSRQSVHLQTVQ
                     GQFSGSGYIGNGVNVVTIMRNHNELLTRQAAAAQAVQAGDTVRAERLTQLQDVFQGGA
                     TGLGAAISDMLNSLVDVVAAPSDITARTVTLTRMNEMASRMRNSGNQLHELEYSVNEQ
                     LTNNATRINQLAQSIAEANEQVARAKGNGQSPNDLLDRRDQLIRELNQYIQTTQIPAD
                     DGSIGVFIGGSQALVLGNQAASVSVAESQLFTGSNQMRLFFNRPGATPSEMQEDMLGG
                     GEMAGLLKFANNDLVEGRNLLGRMALAIGMSMNAQNKLGLTLDGKVGGDLFTVPAGAK
                     GYTSSAGLAGSNVSYADPTRFAASDYEVRFTGANAGQVVRLSDGKTTGFTSLTDPSLQ
                     NIDGLTFNLTGAGAAGERILFKPFADAAANIKALVNSPRDLAAANPINAAMGTSNGGT
                     LQL"
     gene            complement(1373..2308)
                     /gene="flgJ"
                     /locus_tag="PK518_15005"
     CDS             complement(1373..2308)
                     /gene="flgJ"
                     /locus_tag="PK518_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HRM50268.1"
                     /translation="MSIPLNPSTTVNARQALAVDGRSLNALKFQSTQGDAQATQGAVQ
                     EAAKQLESLFMRELIKSMREATAKSGLLDGAEGNLANDLLDQQLSVQMSGLPGGLSDA
                     IQRQLSKQLGGDGQTALVPGSTLSLDSTLRKAAPADNPRARGPKGRDDFVQHHRAAAE
                     RVAQDSGIPASFMLGQAGHETGWGKSEIRNKDGSNAFNLFGIKAGKGWTGKVAEVTTT
                     EYVNGVPRKTVAKFRAYDSYEDSFKDYARLINDSPRYEKARAKTHSAVAFAAELQKAG
                     YATDPQYASKLSRAIQSTHGIARSPLLARATGTQA"
     gene            complement(2308..3453)
                     /locus_tag="PK518_15010"
     CDS             complement(2308..3453)
                     /locus_tag="PK518_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRM50269.1"
                     /translation="MTTLALPLSSTAIGALRQLAVFGLLALAAAQPAHALRIKEVAAV
                     QGVRSNQLTGYGLVVGLDGTGDQTTQMPITTQAMQNYLQQAGISLPAGATAPQLKNVA
                     TVIVTAQLPAFAQPGQSIDVTVASMGNAKSLKGGTLIATPLRGADGEIYALAQGNVVV
                     GGAGASAGGSKVQVNHLSAGRVPDGAQVERSVPTPLHEGETIKLGLNASDFQTARKVA
                     RVINERLGGGTATALDGRTVQVQAPQDPGARVNFIAELEELPLPDSTPAAKVVINPRT
                     GSIVLNQAVTLGPCAIAHGNLSITISSTPVISQPAPLSQGQTVVAQKSDIQIKQDGGK
                     VLQVPASPQLADVVRALNTLGATPQDLLAILQAIKAAGALNAELEVI"
BASE COUNT          474 a         1183 c         1257 g          677 t
ORIGIN      
        1 gagctgcagc gtgccgccat tggacgtgcc catggcggcg ttgatggggt tggcggccgc
       61 caggtcgcgc ggcgaattca ccagggcctt gatgttggcc gccgcgtcgg cgaagggctt
      121 gaacaggatg cgctcgcccg ccgcgcccgc gccggtgagg ttgaacgtca ggccgtcgat
      181 gttctgcagg ctcggatccg tgaggctggt gaagcctgtg gtcttgccgt cggacaggcg
      241 caccacctgg cccgcattgg ctccggtaaa gcgcacctcg tagtccgatg cggcgaagcg
      301 cgtcgggtcg gcatagctga cattgctgcc cgccaggccg gcgctgctgg tgtagccctt
      361 ggcgccggcg ggcactgtga acaggtcgcc accgaccttc ccgtccagcg tcaggcccag
      421 cttgttctgc gcgttcatgc tcatgcctat ggccagcgcc atgcgcccga gcaggttgcg
      481 cccctccacc aggtcgttgt tggcaaactt cagcaggccg gccatctcgc cgccgcccag
      541 catgtcctcc tgcatctccg acggcgtggc gccggggcgg ttgaagaaca ggcgcatctg
      601 gttgctgccc gtgaacagct gtgattcggc caccgacacc gatgccgcct ggttgcccag
      661 caccagcgcc tggctgccgc cgatgaacac gccgatggag ccatcgtcgg ccggaatctg
      721 cgtggtctgt atgtattggt tcagctcgcg tatcagctgg tcacgccggt ccagcaggtc
      781 gttgggcgac tggccgttgc ccttggcgcg cgccacctgc tcgttggcct cggcaatgct
      841 ttgcgccagc tggttgatgc gcgtggcgtt gttggtgagt tgctcgttca ccgagtattc
      901 gagctcgtgc agctggttgc cgctgttgcg catgcgcgat gccatctcgt tcatgcgcgt
      961 cagcgtcacg gtacgcgccg tgatgtcgga gggcgcggcc accacgtcca ccagcgagtt
     1021 gagcatgtcg gatatcgccg cccccaggcc tgtggccccg ccctggaaca cgtcctgcag
     1081 ctgcgtcagg cgctcggcgc gcaccgtgtc gccggcctgc acggcctgcg cggcggcggc
     1141 ctggcgcgtc agcagctcgt tgtggttgcg catgatggtg accacgttga cgccgttgcc
     1201 gatgtagccc gagccgctga actggccttg caccgtctgc aggtgcacgc tctggcgcga
     1261 atagccggcg gtgctgacgt tggcaatgtt gtgccccgtg gtctgcaggg ccgtctggtt
     1321 ggcgagcagg gcgcgggcac cgacgctgag caggctcatg atctacccct tgtcaggcct
     1381 gtgtgcctgt ggcccgggcc agcagcggcg agcgcgctat gccgtgcgtg ctctggatgg
     1441 cgcggctcag cttgctggcg tattgcgggt cggtggcgta gccggccttt tgcagctctg
     1501 ccgcaaaggc cacggccgaa tgggtcttgg cgcgcgcctt ctcgtagcgc gggctgtcgt
     1561 tgatcaggcg cgcatagtcc ttgaacgagt cctcgtagga gtcgtaggcg cggaatttgg
     1621 ccaccgtctt gcgcggcacg ccgttcacgt actcggtggt ggtcacctcg gccaccttgc
     1681 cggtccagcc cttgccggcc ttgatgccga acaggttgaa ggcgttgctg ccgtccttgt
     1741 tgcggatctc gcttttgccc cagccggtct cgtggccggc ctggcccagc atgaagctgg
     1801 cggggatgcc actgtcctgc gccacgcgct cggcggcggc gcggtggtgc tgcacgaagt
     1861 cgtcgcgccc cttggggccg cgcgcgcgcg ggttgtcggc gggggcggcc ttgcgcagcg
     1921 tgctgtccag gctcagcgtg gagccgggca ccagggcggt ctggccgtcg ccgccgagct
     1981 gcttggagag ctggcgctgg atggcgtccg acaggccgcc gggcaggccc gacatctgca
     2041 ccgacagctg ctggtcgagc aggtcattcg ccaggttgcc ctcggcgcca tcgagcaggc
     2101 cggacttggc cgtggcctcg cgcatgctct tgatcagctc gcgcatgaac agcgattcga
     2161 gctgcttggc ggcctcttgc accgcaccct gggtggcctg cgcgtcgccc tgggtggact
     2221 ggaatttcag cgcattcagc gagcgcccat cgacggccag cgcctggcgg gcgttgaccg
     2281 tggtgctggg gttcagcggg atgctcatca gatcacctcc agctcggcat tcagcgcgcc
     2341 cgccgccttg atggcctgca ggatggccag caggtcctgc ggcgtggcgc ccagggtgtt
     2401 gagggcgcgc accacgtcgg ccagttgcgg cgaggccggc acctgcagca ccttgccgcc
     2461 gtcctgcttg atctggatgt cgctcttttg tgcgaccacg gtctggccct gcgacagcgg
     2521 tgccggctgg ctgatgaccg gcgtgctgct gatggtgatg gacaggttgc catgggcgat
     2581 ggcgcagggc cccagcgtca ccgcctggtt gagcacgatg gagccggtgc gcgggttgat
     2641 caccaccttg gccgcagggg tggaatcggg cagcggcagc tcttcgagct cggcaatgaa
     2701 gttcacgcgc gcaccggggt cttgcggtgc ctgtacctgc accgtacgac cgtcgagcgc
     2761 cgtggccgtg ccaccgccca ggcgctcgtt gatgacgcgc gccaccttgc gcgcggtctg
     2821 gaagtcggat gcgttcaggc ccagtttgat ggtctcgccc tcgtgcagcg gcgtgggcac
     2881 gctgcgctct acctgggcgc catcgggcac gcggccggcg ctcaaatggt tgacctgcac
     2941 cttggagccg ccggccgatg cgcccgcgcc gcctaccacc acgttaccct gggccagggc
     3001 gtagatctcg ccatccgcgc cgcgcagcgg cgtggcaatc agcgtgccgc ccttgagcga
     3061 cttggcattg cccatcgagg ccacggtcac gtcgatgctt tgccccggct gggcaaaggc
     3121 cggcaactgc gcggtgacga tcaccgtggc cacgttcttg agctgcggcg ccgtggcgcc
     3181 cgcgggcagg ctgatgccgg cctgttgcag gtagttctgc atggcctggg tggtgatagg
     3241 catctgcgtg gtctggtcgc ccgtgccgtc cagccccacc accaggccgt agcctgtgag
     3301 ctggttgcta cgcacgccct gcaccgccgc cacctccttg atgcgcagcg catgcgccgg
     3361 ctgcgccgca gccagcgcca gcagcccgaa cacggccagc tgccgcagcg cgccgatggc
     3421 cgtcgatgaa aggggcaggg caagcgtggt catggcaatg attgtgggcg tgtgcctcgc
     3481 catgcaaagc gctgaaaaga tgccggaaag cccgttaatt gccggacacc gacgccaaga
     3541 actaatcgga attaatgggg gtcaaattcc aattaatttt ggcctctagc g
//
LOCUS       DAOMTQ010000231         3589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7200_length_3589_cov_26.990096, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000231 DAOMTQ010000000
VERSION     DAOMTQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7200_length_3589_cov_26.990096"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PK518_15015"
     CDS             complement(<1..1019)
                     /locus_tag="PK518_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50270.1"
                     /translation="MGWLSLLPAGAVRTLRGFLLKQGRRMGGAGMLARSKSSALRACM
                     DAARHSPAYRTLLQESGINPSGIGPQTDLTSLPVLTKANTFERFALAQLARPLPATTL
                     ADVLTSSGRGGRSYGFRLTAREQYEEAWFDIDLGLQDIFGVDDKPTLLVNCLPMGVVF
                     RSRAVAVANVSVREDMACSILRDVGPGFAQTLLCTDPMFIRRLLDEAQAVGVDWRALG
                     TSVILGEEVLVETQRDYIAARMGIAIDHDPHRLVGSSFGVGELGLNLLFETRETIRMR
                     RAMRGNPALAQLLCGGAPPDAMPSVFCYNPLRTHLEVLNPDANGFGELCITMLGAGNV
                     IALPR"
     gene            complement(1025..1756)
                     /locus_tag="PK518_15020"
     CDS             complement(1025..1756)
                     /locus_tag="PK518_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HRM50271.1"
                     /translation="MPAVSQPQQKFSVNFSGHAVQPSRDKAFFKSIEELRYQVYCEEC
                     GFLAPDDYPEKRETDEHDANSAHFAALNEAEQLVGYVRLVFPDALQSFPFQTHCVSIL
                     DNVILPPPAQSAEISRLMVHQNYRRRLGELPPAGFPLAETPAEAPRANDQRNPSPQIM
                     LTLYREMYVYSVNNNIRYWYAAMERSLARILARMNFGFQQIGPFTDYYGPVAPYVADL
                     RVLEYQVGQRDPALLAWMRAPLAQS"
     gene            2485..>3589
                     /locus_tag="PK518_15025"
     CDS             2485..>3589
                     /locus_tag="PK518_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM50272.1"
                     /translation="MHRTSLAADRLPPGLLGTQGVTAPDEGVVADMALINTLLQQGKI
                     DQALKAIDALQKKRPADVVPPFLRGRALLQKGDKAGARKAMEQALQIDANFFPAIGVL
                     AVIDNAENHPDEARARLEAAIGQQPGNIQAYQLLLELRAAHGADKAELTAILRRAVEA
                     APKNPLAHQFLVEFLLRSGEHKEALAAAQNAVAALPDKAQLLDAQGRAQAANGEFDQA
                     QASFNRMAALQPQSPQPYLRMSTAYLMAGDRAAAADKLRKALDIDPSLLEAQQGLVNL
                     AMAANKPDEALAIARTVQKQRPREQAGYRLEGDIHAAGKAWDQAAEAYRTGIKLSDSP
                     ELAVRLHSALQSAGKKADADRWAADWTRSHPKDA"
BASE COUNT          674 a         1115 c         1166 g          634 t
ORIGIN      
        1 tagcgcggca gggcaatgac gttgccggcg cccagcatgg tgatgcacag ctcgccaaag
       61 ccattggcgt cgggattcag cacctccagg tgggtgcgca gcgggttgta gcagaacacc
      121 gagggcatgg cgtccggtgg cgcaccgccg cacagcagct gcgccagggc cgggttgcca
      181 cgcatggcgc ggcgcatgcg tatggtctcg cgcgtttcaa acagcaggtt caggcccagc
      241 tcgcccacgc cgaacgagct gcccaccagg cggtgcgggt cgtggtcgat ggctatgccc
      301 atgcgcgcgg cgatgtagtc gcgctgggtc tccaccagca cctcttcgcc cagaatcacg
      361 ctggtgccga gggcgcgcca gtccacgccc acggcttgtg cctcgtccag cagccggcgg
      421 atgaacatcg ggtcggtgca cagcagggtt tgtgcaaagc ctgggcccac gtcacgcagg
      481 atggagcagg ccatgtcctc gcgcacgctg acattggcca cggccaccgc gcgcgagcga
      541 aacaccaccc ccatgggcag acagttgacg agcagcgtgg gcttgtcgtc cacgccaaaa
      601 atgtcctgca ggcccaggtc gatgtcgaac caggcctctt cgtactgttc gcgcgccgtc
      661 aggcgaaagc cgtagctgcg tccgccccga ccggagctgg tcagcacatc ggccagcgtg
      721 gtggcgggca gcggccgggc cagctgggcg agcgcaaagc gctcgaaggt gttggccttg
      781 gtgagtacgg gcaggctggt tagatcggtt tgggggccaa tgccggatgg gttgatgccg
      841 ctttcttgca gcagggtccg gtaggccggg ctatgccggg cggcgtccat gcaggcgcgc
      901 agcgcgctgc tttttgagcg ggcaagcatg cctgcgccgc ccatgcgccg gccctgcttc
      961 aacaaaaagc cgcgcagggt gcgcacggcg ccagcgggca gcagcgagag ccagcccatt
     1021 cagttcaaga ctgtgccagc ggcgctcgca tccaggcgag caatgccggg tcgcgctggc
     1081 caacctggta ctcaagcacc cgaagatctg cgacgtaggg ggccacgggg ccgtagtagt
     1141 cggtaaacgg gccaatctgc tgaaaaccga agttcatgcg ggcgagtatg cgcgccaggg
     1201 agcgctccat ggcagcgtac caataacgga tgttgttgtt cacgctgtac acatacatct
     1261 cgcggtacag cgtgagcatg atttgcggcg atgggttgcg ctggtcgttg gcccgcggcg
     1321 cttcggcggg cgtttcggcc aatggaaacc cggcgggggg cagttcgcca agacgccggc
     1381 ggtagttctg gtgcaccatc agcctgctga tttcggccga ctgcgcgggc gggggcagga
     1441 tgacgttgtc gagaatcgag acgcagtggg tctggaacgg aaagctctgc aacgcgtcag
     1501 gaaacaccag ccgcacgtac cccaccagct gttctgcttc attcagcgcc gcgaaatgcg
     1561 cagagttggc gtcgtgctcg tccgtttcac gcttctcggg gtagtcatcg ggtgccagga
     1621 agccgcattc ctcacaatag acttggtagc gtagctcctc tatggacttg aaaaatgcct
     1681 tgtcgcgact gggttgcacg gcgtggccgg agaagttgac cgagaacttt tgttgaggct
     1741 gcgaaacggc aggcatgcga gctgctcctt ctggtatttg gacgactgga gggttctggc
     1801 gacgagatgc ggcgcctttt cagcgggccg cgcatgccaa aaaaagatga ttgggcaggc
     1861 tcgctgcgca atctgcgtcc atgatcctgc agtacggcaa atccatggat attcttagtc
     1921 aaattcgtaa caaaataata tttgatagct agttatgact atgtcgatct cgcttgtata
     1981 cggtcttgat gttcatcaat tcgatggcat tctctgatac gcctgttgct gtattgtggg
     2041 cttactcagt ggtgttttcc cttgatgacc tgtcagaggg catgcgggtg gttgccaaag
     2101 cgatgcgcca tggccggttg gggctgcact tgcatatcgc tatcgaaatt tgccatttgg
     2161 caacattttc tggagcatcc agcggttgcc ccaggcggcg gccgcgacgg gctatcaaac
     2221 tcccaggtgc ttcggggcaa actatcatcg tcagcgcgct ggctctgccg ggttcatgcc
     2281 aatgtcggcc gttgaactca tggagcttgg caacggtgcc aataccccgg tacaaaaaaa
     2341 cttcctggag aacgaaaaat gatggctatc gcgtcccgca ttgttttttt tgcggcctct
     2401 cttgcaactg ccctgccggt atccgctgcc agcgacgccg cgggtcagtc ggccaacgca
     2461 tccagcggca cgccgcaagg cctcatgcat cgcacctctt tggctgccga ccggctgcca
     2521 ccggggttgc tcggcacgca aggcgtcact gcgcccgatg agggtgtggt cgcggatatg
     2581 gcgctgatca acaccctgct gcagcagggc aagatcgacc aggcgctcaa ggccatagac
     2641 gcgctgcaaa aaaagcgtcc cgccgacgtg gtgccaccct tcctgcgcgg gcgcgccctg
     2701 ctgcaaaagg gcgacaaggc cggcgcacgc aaggccatgg agcaggcgct gcagatcgat
     2761 gccaacttct tcccagccat cggcgtgctg gcggtcattg ataacgccga aaaccacccc
     2821 gacgaagccc gcgcccggct ggaggccgcc atcgggcagc agccgggcaa tatccaggcc
     2881 taccagttgc tgctggagct gcgcgccgcc catggcgccg acaaggccga gctgaccgcc
     2941 atcctgcgcc gtgcggtgga ggccgcgcct aagaacccgt tggcgcatca gtttctggtt
     3001 gagttcctgc tgcgcagtgg cgagcacaaa gaggccctgg ccgcggcgca gaatgccgtt
     3061 gccgcattac ccgacaaggc gcagttgctt gacgcccagg ggcgcgcaca agcggcgaat
     3121 ggtgaatttg accaggccca ggccagcttc aaccgcatgg cggcgctgca accccagtcg
     3181 ccccagccct acctgcgcat gtccaccgcc tacctgatgg cgggcgaccg cgccgcggcc
     3241 gcggataagc tgcgcaaggc gctcgatatc gaccccagcc tgctcgaggc ccagcaaggc
     3301 ctggtcaacc tggccatggc ggccaacaag cctgacgaag cactggccat cgcccgcacg
     3361 gtgcagaaac aacggcccag ggagcaggca ggttatcgcc tggaaggtga tatccacgcc
     3421 gctggcaagg cctgggatca agcggccgag gcttaccgta cgggcatcaa gctgtccgac
     3481 agccccgagc tggccgtgcg cctgcacagc gccctgcaga gcgccggcaa gaaagcggat
     3541 gccgaccgct gggccgcgga ctggacacgc agccacccca aggacgcca
//
LOCUS       DAOMTQ010000232         3589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7201_length_3589_cov_25.245614, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000232 DAOMTQ010000000
VERSION     DAOMTQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7201_length_3589_cov_25.245614"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK518_15030"
     CDS             complement(<1..629)
                     /locus_tag="PK518_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRM50273.1"
                     /translation="MGANSQIQLKTTATAGGSVVTTPQVRAELVAQAPEGVAPGRPLQ
                     LGLLLTHSPDWHTYWKNPGDSGLPTQLEWQLPAGLDAGEIAWPVPKKISIGHLANYGY
                     EGTALLPVPVTVSPAFNPGPLAQEVTIRLKASWLVCRQECIPEDGEFTLQLPIQGTTA
                     LHAAAFDAAAQAHPQNLPAHADSGARVDGGAVLLRVAGLPGALQGQKLEV"
     gene            769..1602
                     /locus_tag="PK518_15035"
     CDS             769..1602
                     /locus_tag="PK518_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRM50274.1"
                     /translation="MSYPSRFWADLSTRDFAEARTWGLAAQTVAVLPVAAVEQHGPHL
                     PLSVDAALLDGVIQAALPLLPAELPVLFLPPQQLGLSTEHRSYPGTLTLSPSTLIALW
                     TELGECVARAGIKKLLLLNGHGGNVSPMDIVARELRQRCGLLVYSSSWFGLPLPDAVS
                     GLFSAQEHRFGIHGGEIETSMMLHLAPETVHMEHARHWRSTSQDRAERHAILGNGKSA
                     KMGWAMEDYHSAGAVGNAAAASADKGQAVVQAAGAALAQLLAELHALPLSTLAPRPGP
                     P"
     gene            1699..2898
                     /locus_tag="PK518_15040"
     CDS             1699..2898
                     /locus_tag="PK518_15040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM50275.1"
                     /translation="MLQIRPPLQASTTKYAQQLRRGFRWLRFDAPLEAEYRAYSHSDE
                     YVRVLWFSALCLLLWGGYGLFDLWRIDAARLGAGQQPLLTALRVTRVAVTVAIALVLV
                     GALWRAPMRWLHGALMGLGVALSCGAAFAVYAYKVFGVPHETAVLVMIMLGLFSPFAI
                     NLWAQLAMAALYLACVGALAWVAPQPEVQAQMLRLGVELALAALVFSFAAYWREHQRR
                     EQFLYRGDAHWLAMRDALTGLYNRRMFNHHLEQVMAQARREGQPLALLIVDVDHFKAY
                     NDLHGHPQGDAVLQQVAQVGLACIGRPLDIAARVGGEEFAVLLYGSSAEHARALGQSL
                     VAGVRQRALAHGASPVADVVTVSVGGAMVQPEDRPQTLFARADRVLYQAKNNGRDRCH
                     WEAALQA"
     gene            complement(2889..3560)
                     /locus_tag="PK518_15045"
     CDS             complement(2889..3560)
                     /locus_tag="PK518_15045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM50276.1"
                     /translation="MGTLYLVRHGQASFGADDYDQLSARGQQQSERLGAYWRERGLRF
                     DAVLCGTLRRHAQTLAGIAQGLGAMPEPQLLPGLNEYNSQALISAIHPAPLPRPDTPE
                     LYRQHFRLLCDALAQWMAGTISPAGMPGWDGFSGGVRAALEQVRRQHVGGSVLLVSSG
                     GPIAAAVGEVLRTPPEVTIALNMRIRNSAVTEFSVSPKRLMLQTFNSLAHLDAPEYAG
                     WVTHA"
BASE COUNT          497 a         1178 c         1322 g          592 t
ORIGIN      
        1 acctccagct tttgcccctg cagcgcgccg ggcaggccgg ccacgcgcag cagcacggcg
       61 ccgccgtcca cgcgcgcgcc gctgtccgcg tgcgcgggca ggttttgcgg atgggcctgg
      121 gcggcggcgt caaaggccgc ggcgtgcagg gcggtcgtgc cctgtatggg cagctgcagg
      181 gtgaattccc cgtcctcggg aatgcattcc tggcggcaca ccagccacga ggccttgagc
      241 cggatggtga cctcctgcgc cagcgggccg gggttgaagg ccggcgacac ggtcacgggc
      301 acgggcagca gtgccgtacc ctcgtagccg tagttggcca ggtggcctat ggagatcttt
      361 ttgggcacgg gccaggcgat ctcgcccgca tccaggccgg cgggcagctg ccattccagc
      421 tgcgtgggca ggcccgagtc gccggggttc ttccagtagg tgtgccagtc agggctgtgg
      481 gtcagcagca ggcccagctg cagcggccgg cccggggcca cgccctcggg ggcctgggcg
      541 acgagctcgg cacgcacctg gggggtggtt acgacgctgc cacctgccgt ggccgtggtt
      601 tttagctgta tttggctgtt ggcgcccatg ggtagcgcgc tgacagctat aaaaacaata
      661 gttggcagga tctgccgtag aagtcggtga agcatgtcgc agggtgggag cggcggggcg
      721 ccgtttggtt ccttggggca atggtagagc cacattaaga tggctggcat gtcatacccc
      781 tcccgtttct gggccgacct gtccacgcgt gactttgccg aggcgcgtac ctggggcctg
      841 gccgcgcaga ccgtggctgt gctgcccgtg gcggcggtgg agcagcatgg gccgcatctg
      901 ccgctgtcgg ttgacgctgc gttgcttgac ggcgtgatcc aggcggcgct gccgctgctg
      961 ccggccgagt tgccggtgct gtttttgccg ccacagcagc tgggcctgtc caccgagcac
     1021 cgctcctacc ccggcacgct gacgctgtcg cccagcacgc tgatcgcgct gtggaccgag
     1081 ctgggcgaat gcgtggcgcg cgccggcatc aagaagctgc tgctgttgaa cggccatggt
     1141 ggcaacgtga gccccatgga catcgtggcg cgcgagctgc gccagcgctg cggcctgctg
     1201 gtctacagca gcagctggtt cggcctgccg ctgccggacg cggtgagcgg gctgttcagc
     1261 gcgcaggagc accgctttgg catccacggc ggcgagatcg agacgtcgat gatgctgcac
     1321 ctggcgcccg agaccgtgca catggagcac gcgcgccact ggcgctcgac atcccaggat
     1381 cgcgccgagc gccatgccat cctgggcaac ggcaagagcg ccaagatggg ctgggccatg
     1441 gaggactacc acagcgccgg cgccgtgggc aacgccgccg ccgccagcgc cgacaagggc
     1501 caggccgtgg tgcaggcggc gggcgccgcg ctggcgcagc tcctggccga gctgcatgcg
     1561 ctgccgctgt ccaccctggc gcccaggccg gggccgccgt agccggccgg tgctggcttg
     1621 gcactactgt gatagcgcac atcaaggcgc aaaatgaagc acagtggtac aaccggccgc
     1681 attgggagaa tcaaccgcat gcttcagatc aggccgccct tgcaggcatc caccaccaag
     1741 tacgcacagc agctgcgccg gggctttcgc tggctgcgct tcgatgcgcc gctggaggcc
     1801 gaataccgcg cctatagcca cagcgacgag tatgtgcgcg tgctgtggtt cagcgccctt
     1861 tgcctgttgc tgtggggtgg ctacgggctg tttgacctgt ggcgcatcga tgccgcgcgg
     1921 ctgggcgccg ggcagcagcc gctgttgacc gccctgcgcg tgacgcgcgt ggccgtcacg
     1981 gtggcgattg cgctggtgct ggtcggcgcc ttgtggcgcg cgcccatgcg ctggctgcat
     2041 ggggcgctga tggggctggg cgtggcgctc agctgcggtg cggcgtttgc ggtgtatgcc
     2101 tacaaggtgt tcggcgtgcc gcatgagacg gccgttctgg tgatgatcat gctgggcctg
     2161 ttttcgccgt tcgccatcaa cctgtgggcg caactggcca tggccgcgct gtatctggct
     2221 tgcgtgggtg cgctggcctg ggtggcaccg cagcccgagg tgcaggcgca gatgctgcgc
     2281 ctgggcgtgg agctggcgct ggcggcgctg gtgttcagct ttgccgccta ctggcgcgag
     2341 caccagcggc gcgagcagtt cctgtaccgc ggcgacgccc actggctggc gatgcgcgat
     2401 gcgctcaccg gcctgtacaa ccgccgcatg ttcaaccacc acctggagca ggtcatggcc
     2461 caggcgcggc gcgagggcca gccgctggcc ttgttgatcg tcgatgtgga tcacttcaag
     2521 gcctacaacg acctccatgg ccacccgcag ggcgacgccg tgctgcagca ggtggcgcag
     2581 gtcggcctgg cctgcattgg ccggccgctg gacatagcag cgcgcgtggg cggcgaggag
     2641 tttgccgtgc tgctgtacgg cagcagcgcc gaacacgccc gggcgctggg acaaagcctg
     2701 gtggccggcg tgcgccaacg cgcgctggcc catggggctt caccggtggc cgatgtggtc
     2761 acggtgagcg tgggcggcgc catggtgcag cccgaagacc gcccgcagac gctgtttgca
     2821 cgcgccgacc gggtgctcta ccaggccaag aacaacgggc gcgaccgctg ccactgggag
     2881 gcggcgcttc aggcgtgagt gacccagccc gcgtattcgg gcgcgtccag gtgcgccagg
     2941 ctgttgaagg tctgcagcat caggcgtttg gggctcacgc tgaactcggt gacggcgctg
     3001 ttgcggatgc gcatgttcag cgcaatcgtc acctcgggcg gcgtgcgcag cacctcgccc
     3061 acggcggcgg caatcggccc gccgctggag accagcagca cgctaccccc cacatgctgg
     3121 cgccgcacct gctccagcgc ggcgcgcacg ccgcctgaaa acccatccca gccgggcatg
     3181 cccgccgggc tgatggtgcc ggccatccac tgcgccagcg cgtcgcacag caggcggaaa
     3241 tgctgcctgt acagctcggg tgtgtccggg cgcggcaggg gcgcggggtg gatggcgctt
     3301 atcagcgcct ggctgttgta ttcgttcagg ccgggcagca gctggggctc gggcatggcg
     3361 cccaggccct gggctatgcc cgccagcgtc tgcgcgtggc ggcgcagcgt gccgcagagc
     3421 acggcgtcaa agcgcaggcc gcgttcgcgc cagtaggcgc ccaggcgctc ggactgctgc
     3481 tggccgcgcg cgctcagctg gtcgtagtca tccgcgccaa acgaggcctg gccgtggcgc
     3541 acgaggtaga gggttcccat gccggcgatt ttgatcaaac aggccgctg
//
LOCUS       DAOMTQ010000233         3577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7234_length_3577_cov_24.270017, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000233 DAOMTQ010000000
VERSION     DAOMTQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7234_length_3577_cov_24.270017"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PK518_15050"
     CDS             <1..1224
                     /locus_tag="PK518_15050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRM50277.1"
                     /translation="WSLPYTNLAGKFNANSQLLSLWPWAAALLLASWQERGLRGAWYS
                     AWLGLVAAAAMLSKYYSGVFLAGFLLPTLLHADGRRWLTTPRPYLALAVFGLALAPHL
                     AWVAAHDWAPLGYAMDQGGGGTSWPYVARFALAPLFYWLPAWLALCLVWARVQARHDG
                     APWGRTAARLLLRSWLPRGWDDLLFWLACMPWALTLAFGAAGVVELSTPWAIPIGYAF
                     ALLWLRNLQLLAPAAAEGTLQRLGRAWWPVLALVLLLGVVAAMVSALRGDQGYYRPTP
                     EAARAIVDDWQRRHPGQRLQWVGGAWAENAMLAFYAQPGLLTVPGLPDTELARLSALA
                     DWQRRNGLLLCPLGPVADPQPPSDCQRQAEDWLAARGQPVQAQRLQVQRGGWRFPRPQ
                     AWAYAVYDVDAAPAR"
     gene            1241..2278
                     /locus_tag="PK518_15055"
     CDS             1241..2278
                     /locus_tag="PK518_15055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM50278.1"
                     /translation="MTETPAPYRVTPNADYHADYAPAAPPAQLPRAPLRLSCVVPAHN
                     EEANLEDFVRALAQAAGRLTPEFEIIIVNDGSRDATHAVALRLAQDLPVRYLALSRNF
                     GKEAALTAGLAHARGSAVLLIDADFQHPLELLPEMHALWLAGYDMVYGVIVDRGFESG
                     TKRVGTGLFYRLINTGKQVKVPPNAGDFRWMDRRVADALNALPERNRFMKGLYAWVGF
                     KAAALPFVPHDRAGGQTSFSMRRLGSLALLGLTSFTTLPLRVWSVVGSLVALLALVYG
                     AWITLDTILHGTDLQGWPTLAAGIMLFSGVQLMSIGILGEYIGRIYEEVKRRPTYLVA
                     RDEDNSPWRDS"
     gene            2275..2682
                     /locus_tag="PK518_15060"
     CDS             2275..2682
                     /locus_tag="PK518_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRM50279.1"
                     /translation="MSAQALASQLTRLPQPLQFALVGGCAAATHLAVVALLVQLTQMA
                     PLVANVLAFLVAFVVSYNGHALLTFSSAGARGWPVVGRYFAVACLSFVGNELLYALAL
                     DWLHWHYLWSLAGVLLLVAVATFVLSKFWAFKA"
     gene            2679..3485
                     /locus_tag="PK518_15065"
     CDS             2679..3485
                     /locus_tag="PK518_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRM50280.1"
                     /translation="MSAGTRGITLCADDYALHPLVDEAVVLLAQAGRLSATSCMSTAP
                     RWREAAPQLLPLRGRIQLGLHFNLTEGHGGAHAAHSLGQVLTAAYTRRLPASALQDAW
                     RAQLDAFEDAIGSAPDFIDGHQHVHQLPGVRRALLAELQRRYAGRAMPWVRSTAPAGR
                     LWRDPKAFVIALLGGWSATRLLRRAGVLQNQGFGGVYGFDAATPGEYGALMQAWLEEA
                     RDGSLLMCHPATAEVPGDAIGRQRPVEFAYLMSDAFAQALQRQGCQIKQF"
BASE COUNT          435 a         1293 c         1267 g          582 t
ORIGIN      
        1 tggagcctgc cctacaccaa cctggcgggc aagttcaacg ccaacagcca gctcttgtcg
       61 ctctggccct gggccgcggc cctgctcctg gccagctggc aggagcgcgg cctgcgcggc
      121 gcctggtaca gcgcctggct gggcctggtg gccgcggcgg ccatgctgag caagtactac
      181 agcggcgtgt tcctggccgg ttttctgctg cccacgctgc tgcacgccga cggccggcgc
      241 tggctgacca cgcccaggcc ctacctggcg ctggcggtct ttggcctggc gctggcgccg
      301 cacctggcct gggtggcggc gcacgactgg gcgccgctgg gctatgccat ggaccagggc
      361 ggcggcggca cctcctggcc ctatgtggcg cgctttgcgc tggcgccgct cttttactgg
      421 ctgcccgcct ggctggcgct gtgcctggtc tgggcgcggg tgcaggcgcg ccatgatggc
      481 gcgccctggg gccgcacggc ggcacgcctg ctgctgcgca gctggctgcc gcgcggctgg
      541 gacgacctgc tgttctggct ggcctgcatg ccctgggcgc tgaccctggc ctttggcgcg
      601 gccggcgtgg tggagctgtc caccccttgg gcgataccca ttggctacgc gtttgcgctg
      661 ctgtggctgc gcaacctgca gctgttggcg ccggcggcgg ccgaggggac gctgcagcgc
      721 ctgggccggg cctggtggcc ggtgctcgcg ctggtgctgc tgctgggcgt ggtggccgcc
      781 atggtcagcg ccctgcgcgg cgaccagggc tattaccggc ccacgccgga ggccgcgcgc
      841 gccatcgtcg atgactggca gcggcgccac cccggccagc gcctgcaatg ggtgggcggc
      901 gcctgggccg agaacgccat gctggccttc tacgcccagc ccggcctgct gaccgtgccc
      961 ggcctgccag acaccgagct ggcgcgcctg tcggccctgg ccgactggca gcggcgcaac
     1021 ggcctgctgc tgtgcccgct ggggccggtg gccgacccgc aaccgcccag cgactgccag
     1081 cgccaggccg aggactggct ggccgcgcgc ggccagccgg tgcaggcgca gcggctgcag
     1141 gtgcagcgcg gcggctggcg ctttccgcgc ccgcaggcct gggcctatgc ggtgtatgac
     1201 gtggatgcag cccctgcacg ctgaacagac aatcgacacc atgaccgaaa cgcccgcccc
     1261 ctaccgcgtc acgccgaatg cggactacca cgccgactac gccccggccg cgccgcccgc
     1321 gcaactgccg cgtgcgccgc tgcgcctgag ctgcgtggtg ccggcgcaca acgaggaggc
     1381 caacctcgaa gactttgtgc gcgccctggc gcaggcggcg ggccggctca cgcccgagtt
     1441 tgaaatcatc atcgtcaacg acggcagccg cgacgccacc cacgccgtgg cgctgcgcct
     1501 ggcgcaggat ctgccggtgc gctacctggc cctgtcgcgc aacttcggca aggaggcggc
     1561 gctgacggcc ggcctggcgc acgcgcgcgg cagcgccgtg ctgctgatag atgccgattt
     1621 ccagcatccc ctggagctgc tgcccgagat gcacgcgctg tggctggcgg gttacgacat
     1681 ggtctatggc gtgatcgtcg atcgcggctt tgagagcggc accaagcgcg tcggcaccgg
     1741 cctgttctac cgcctgatca acaccggcaa gcaggtcaag gtgccgccca acgcgggcga
     1801 cttccgctgg atggacaggc gcgtggccga cgccctgaac gccctgcccg agcgcaaccg
     1861 gttcatgaag ggcctgtacg cctgggtggg cttcaaggcc gcggccctcc ccttcgtgcc
     1921 gcacgaccgc gcgggcgggc agaccagctt cagcatgcgc cgcctcggct cgctggcgct
     1981 cttggggttg acctctttca ccaccctgcc gctgcgtgtg tggagcgtgg tcggcagcct
     2041 ggtggccctg ctggcgctgg tctacggcgc ctggatcacg ctggacacca tcctgcatgg
     2101 caccgacctg cagggctggc ccacgctggc cgccggcatc atgctgttct cgggcgtaca
     2161 gctcatgtcc atcggcatcc tgggcgagta catcggccgc atctacgaag aggtcaagcg
     2221 ccgccccacc tacctcgtcg cgcgcgacga ggacaacagc ccctggcgcg actcatgagc
     2281 gcgcaagccc tggccagcca gctcacgcgc ctgccccagc cgctgcagtt cgcgctggtg
     2341 gggggctgcg ccgcggccac gcacctggcg gtggtggcgc tgctggtgca gctcacgcag
     2401 atggcgccgc tggtggccaa tgtgctggcc tttctggtgg ccttcgtcgt cagctataac
     2461 ggccacgcgc tgctcacctt ttctagcgcc ggcgcgcgcg gctggcccgt ggtggggcgc
     2521 tacttcgccg tggcctgcct gtccttcgtg ggcaacgagc tgctgtatgc cctggcgctt
     2581 gactggttgc actggcatta cctgtggagc ctggccggtg tgctgctgct ggtggcggtg
     2641 gccacctttg tcctgagcaa attctgggcc ttcaaggcat gagcgccggc acccgcggca
     2701 tcaccctgtg cgccgacgac tacgcgctgc accccctggt ggacgaggct gtggtactgc
     2761 tggcccaggc cggccgcctg tccgccacca gctgcatgag caccgccccg cgctggcgcg
     2821 aggccgcgcc gcagctattg cccctgcgcg ggcgcatcca gctgggcctg cacttcaacc
     2881 tgaccgaggg ccatggcggc gcccacgcgg cgcacagcct gggccaggtg ctgacggcgg
     2941 cctacacccg gcgtctgccc gccagcgcgc tgcaggacgc ctggcgcgcc cagcttgacg
     3001 ccttcgagga cgccatcggc agtgcgcccg acttcatcga cggccaccag catgtgcacc
     3061 aactgcccgg cgtgcggcgc gccttgctgg ccgagctgca gcggcgctac gccggccgcg
     3121 ccatgccttg ggtgcgctcc accgcgcccg ccggccggct atggcgcgac cccaaggcct
     3181 ttgttatcgc cctgctgggc ggctggagcg ccacacggct gctgcgccgt gccggcgtgc
     3241 tgcagaacca gggctttggc ggcgtgtatg gctttgacgc ggccacgccc ggggagtacg
     3301 gcgcgctgat gcaggcctgg ctggaggagg cgcgtgacgg cagcctgctg atgtgccacc
     3361 ccgccaccgc cgaggtgccg ggtgacgcca taggtcgcca gcgcccggtg gagttcgcct
     3421 acctgatgtc ggacgccttt gcccaggcgc tgcagcggca gggctgccag atcaagcagt
     3481 tctaaaacgg tagctgctgg cgcttgctgg tattgcgctg gcggccggga gcgccggcgt
     3541 taattggggt cagattccaa ttaattttgg cctctag
//
LOCUS       DAOMTQ010000234         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7383_length_3529_cov_21.273172, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000234 DAOMTQ010000000
VERSION     DAOMTQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7383_length_3529_cov_21.273172"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK518_15070"
     CDS             <1..264
                     /locus_tag="PK518_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRM50281.1"
                     /translation="LAADIQSAQAQAGAAAAQEQAMARNAELARATRGFVAKAFSLGE
                     ADWPTRLRVEQDAQLAERQWQRARVDAAAATSTLRQALGLLPE"
     gene            355..891
                     /locus_tag="PK518_15075"
     CDS             355..891
                     /locus_tag="PK518_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50282.1"
                     /translation="MYHRRFLSPALAALLGLSALAVQAHEGHDDAPAAVQTSAAPRFA
                     AVSEQFELVGVIEGHRLSLYLDHAADNSPVPDATLELELGGKPLAVTRVGEGLYQAEL
                     SQPLADGETPVTATVVAGADSDLLAADIDWHAPATAVPPSAWAGKSRLALAGGAVLLA
                     LAALWAWSRRTQRTGGAA"
     gene            888..2000
                     /locus_tag="PK518_15080"
     CDS             888..2000
                     /locus_tag="PK518_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRM50283.1"
                     /translation="MSRQTKYLNALALACALSLPLFVQAHEGHDDAPPALSGDSPRRQ
                     SDGSVFIPKSAQRQLGLRTLAVAAGEHARAFELAGTVVMDPNAGGKVQAALAGRLEAG
                     PQGLPLPGQSVKKGQVLAYVVPTAGALERSGQLAQQAELRAAKELAQRRLARLRELAD
                     TVARKDIEAAESELQSLTARLAAVGAGLQQRDALVAPVSGVIASSNAVAGQVLDAREQ
                     VFEVVDPARLRIEALAYDPAQAQDVAGASLALPGGQSVPLRFLGAARSLREQALPLSF
                     AGDAQALSQLALGQSVRLVVQTRSRVAGLQVPLAALQKNPSNQAIVWVKTGPEQYAPR
                     VVRIQPLDGVHVAVTDGLQPGERVATQGASLLNQIR"
     gene            2010..>3529
                     /locus_tag="PK518_15085"
     CDS             2010..>3529
                     /locus_tag="PK518_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM50284.1"
                     /translation="MFKWLLEKSLAHRLLVLVTAAVLMAYGAYTLSRMPVDVFPDLNK
                     PTVTVMTEAGGMAAEEVEQLITFPLETSMNGLPGVESVRSTSSAGLSFIYVTFDWSVD
                     IFRARQMVSERLSSMDEGLPAGVEPSMGPVSSVMGEIMMVAIPIATERTTPMAVREYA
                     DWVLRPRLLAIPGVAQVIPIGGEVRQFQVQPDTARMAQLGITPEQLSGALKGFSSNTS
                     GGFLEQGGREYLIRHLGRTSRLEDLQNLALAAPNGQSVLLRQVASVSFAPAIKRGDAG
                     FEGQPAVILGIQKQPTADTMALTQRIEQALDELAQTLPAGMDKPRVTFRQASFIEASV
                     TTLQGKLIGASVFVAVILFVFLGTLRPTVIALTAIPVSIFLTALVFKYFGLSINTMTL
                     GGLAIAIGGLVDDAVVDVENVLRRLKMDRHKQGSARLHPLEIVKLASLEVRSGIVYAT
                     AIIVLVFLPLFALPGIEGRLFVPLGVAFIVSTLASLLVSVTVTPVLAFYLLPRMKNL"
BASE COUNT          501 a         1269 c         1225 g          534 t
ORIGIN      
        1 ctggccgccg acatccagtc cgcccaggcc caggccggcg ccgccgccgc ccaggagcag
       61 gccatggcgc gcaacgccga gctggcccgc gccacgcgcg gcttcgtcgc caaggccttt
      121 tctttgggcg aggccgactg gcccacgcgc ctacgcgtgg agcaggacgc ccagctggcc
      181 gaacgccagt ggcagcgcgc ccgcgtggac gccgccgccg ccacctccac cctgcgccag
      241 gcgctgggcc tgctgcctga gtaatcttta agcaaaaaag gcctgcagcg cccttccata
      301 aagcgcaaac agctattaaa tatatagctt acaggccatt gttccgggac cgatatgtac
      361 caccgacgtt ttctttcccc cgccctggcg gccttgctgg ggctgtctgc cctggccgtc
      421 caggcccatg agggtcatga cgatgcgccc gccgccgtgc agaccagcgc cgcgccgcgc
      481 tttgccgctg tgtccgagca gttcgagctg gtcggcgtga tcgagggcca caggctgtcc
      541 ctgtacctgg accatgcggc cgacaacagc cccgtgccgg atgccacgct ggagctggag
      601 ctgggcggca agcccctggc cgtgacccgg gtgggcgagg gcctgtacca ggctgagctg
      661 agccaacccc tggcggacgg cgagactccg gtgaccgcca ccgtcgtcgc cggcgccgac
      721 agcgacctgc tggccgccga catcgactgg cacgccccgg ctacggccgt gccgccatcg
      781 gcctgggcgg gcaagagccg cctggccctg gccggcggtg ccgtcctgct ggccctggcc
      841 gctctgtggg cctggagccg ccgcacgcaa cgcacgggag gtgcagcatg agccgccaaa
      901 ccaaatacct gaacgccctg gccctggcct gcgccctgtc cctgccactg ttcgtccagg
      961 cccacgaggg ccatgacgac gcgcccccgg ccctcagcgg cgacagcccg cggcgccaga
     1021 gcgacggcag cgtattcatc cccaagtcgg cccagcgcca gctgggcctg cgcaccctgg
     1081 ccgtggcggc gggcgagcat gcgcgcgcgt tcgagctggc gggcaccgtg gtcatggacc
     1141 cgaacgccgg cggcaaggtg caggcggccc tggccggccg cctggaggcc ggcccgcagg
     1201 gcctgccgct gccgggccag agcgtgaaga agggccaggt gctggcctat gtggtgccca
     1261 cggcgggtgc gctggagcgc tccggccagc tggcccagca ggccgagctg cgcgcggcta
     1321 aagagttggc ccagcgccgc ctggcgcgcc tgcgcgagct ggccgacacc gtggcgcgca
     1381 aggacatcga ggccgccgag agcgagctgc aaagcctgac ggcgcgcctg gccgccgtgg
     1441 gcgccggcct gcagcagcgc gacgcgctgg tggcgccggt gtcgggcgtc atcgcctcca
     1501 gcaacgccgt cgccggccag gtgctggacg cgcgcgagca ggtgttcgag gtcgtcgatc
     1561 cggcgcgcct gcgcatcgag gccctggcct acgacccggc ccaggcgcag gacgtggccg
     1621 gcgccagcct ggccctgccg ggcgggcaga gcgtgccgct gcgctttctg ggcgcggccc
     1681 gcagcctgcg cgagcaggcc ctgccgctgt cctttgccgg cgacgcccag gccctctcgc
     1741 aactggccct gggccagagc gtgcgcctgg tggtgcagac gcgctcgcgc gtggccgggc
     1801 tgcaggtgcc gctggcggcg ctgcagaaga acccgtccaa ccaggccatc gtctgggtca
     1861 agaccgggcc cgagcagtac gcgccgcgcg tggtgcgcat ccagccgctg gacggcgtgc
     1921 atgtggccgt caccgacggc ctgcagcccg gcgagcgcgt ggccacgcag ggtgcctccc
     1981 tgctgaacca gattcgctaa ggagccgcaa tgttcaagtg gcttcttgaa aaaagtctgg
     2041 cgcaccgcct gctggtgctg gtcaccgcgg ccgtgctcat ggcctatggc gcgtacacgc
     2101 tgtcgcgcat gccggtggac gtgttccccg acctgaacaa gccgacggtc acggtcatga
     2161 ccgaggccgg cggcatggcc gccgaggagg ttgagcagct catcaccttc ccgctggaga
     2221 ccagcatgaa cggcctgccc ggcgtggaga gcgtgcgctc cacctccagt gccggcctgt
     2281 ccttcatcta cgtcaccttt gactggagcg tggacatctt tcgcgcgcgc cagatggtgt
     2341 ccgagcgcct gtcctccatg gacgagggcc tgcccgccgg ggtggagccg agcatggggc
     2401 cggtcagctc cgtcatgggc gagatcatga tggtggccat tcccatcgcc accgagcgca
     2461 ccacgccgat ggcggtacgc gagtacgccg actgggtgct gcgcccgcgc ctgctggcca
     2521 tacccggcgt ggcccaggtg ataccgattg gcggcgaggt gcgccagttc caggtgcagc
     2581 cggatacggc gcgcatggcg cagctgggca tcacgcccga gcagctctct ggcgcgctca
     2641 agggcttctc cagcaacacc tcgggcgggt ttctggagca gggtgggcgc gagtacctga
     2701 tccgccacct gggccgcacc tcgcgcctgg aagacctgca gaacctggcc ctggccgccc
     2761 ccaacggcca gagcgtgctg ctgcgccagg tggccagcgt gagctttgcc ccggccatca
     2821 agcgcggcga tgccggcttc gaggggcagc cggcggtgat cctgggcatt caaaagcagc
     2881 ccacggcaga caccatggcc ctgacccagc gcatagagca ggccctggac gagctggcgc
     2941 agaccctgcc cgccggcatg gacaagccgc gcgtcacctt ccgccaggcc agcttcatcg
     3001 aggcctcggt caccaccctg cagggcaagc tgataggcgc atcggtgttc gtggcggtga
     3061 tcctgttcgt gtttctgggc acgctgcggc ccacggtgat tgcgctcacc gccattcccg
     3121 tgtccatctt tctgacggcg ctggtgttca agtacttcgg cctgtccatc aacaccatga
     3181 cgctgggcgg cctggcgatt gccatcggcg gcctggtgga cgatgccgtg gtggacgtag
     3241 aaaacgtgct gcgccgcctg aagatggacc ggcacaagca gggcagcgcg cgcctgcacc
     3301 cgctggagat cgtcaagctc gcctcgctgg aggtgcgctc cggcatcgtc tacgccacgg
     3361 ccattatcgt gctggtgttt ctgccgctgt tcgcgctgcc gggcatcgaa ggccggctgt
     3421 tcgtgccgct gggcgtggcc ttcatcgtct ccacgctggc gtcgctgctg gtgtcggtca
     3481 ccgtcacgcc ggtgctggcc ttctacctgc tgccgcgcat gaagaacct
//
LOCUS       DAOMTQ010000235         3520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7411_length_3520_cov_25.909668, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000235 DAOMTQ010000000
VERSION     DAOMTQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7411_length_3520_cov_25.909668"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="serC"
                     /locus_tag="PK518_15090"
     CDS             complement(<1..1046)
                     /gene="serC"
                     /locus_tag="PK518_15090"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRM50285.1"
                     /translation="MNRPYNFSAGPAAIPEEVLQRAASEMLDWHGSGMGVMEMSHRGK
                     EFISIYEQAEADLRELLAVPPEFKILFMQGGGLAENAIVPLNLSRAATVDFVVTGSWS
                     QKSRKEAAKYAAEVHTAASGEASGFTTLPDPAGWQLSRGASYVHICSNETIHGVEFHE
                     LPDLKSLGSEAPLVIDFSSHVASRPVDWSRVGLAFGGAQKNIGPAGLTMVIVRDDLLG
                     HALSACPSAFDYRVVADNHSMYNTPPTWGIYMAGLTFQWLKKQREGALSGVAAMEARN
                     IAKARLLYDFIDASPFYVNKVAANCRSRMNIPFFLQDESRNDAFLAGAKARGLLQLKG
                     HKSVGGMRASIYNA"
     gene            complement(1113..>3520)
                     /gene="gyrA"
                     /locus_tag="PK518_15095"
     CDS             complement(1113..>3520)
                     /gene="gyrA"
                     /locus_tag="PK518_15095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM50286.1"
                     /translation="HPHGDIAVYDTIVRMAQDFSLRHMLVDGQGNFGSVDGDNAAAMR
                     YTEIRLSKIAHEMLADIDKETVDFGPNYDGSEKEPLVLPSRLPNLLVNGSAGIAVGMA
                     TNIPPHNLNEVVDACLHLLRHPEATIDELMEIIPAPDFPTAGIIYGIQGVKDGYRTGR
                     GRVVMRAKVHFEDIDRGQRQAIIVDELPYQVNKKTLQERMAELVNDKKIEGISHIQDE
                     SDKSGMRLVIELKRGEVPEVVLNNLYKQTQLQDTFGMNMVALVDGQPRLCNLKDLIEV
                     FIGHRREVVTRRTVFELRKARERGHVLEGLAVALANIDEFIRIIRNAPTPPVAKAELM
                     ARSWDSQLVREMLTRTREDGGVVSADDYRPEGLEREYGMQDGGLYRLSDTQAQEILQM
                     RLQRLTGLEQDKIVAEYKDIMAVIEDLLDILAKPARVSTIIGEELSAVRQEFGQTKLG
                     ARRSEIEHSAQDLSTEDLITPTDMVVTLSHTGYIKSQPLSEYRAQKRGGRGKQAAATK
                     EDDWIDQLFIANTHDYILCFSNRGRLYWLKVWEVPAGSRGSRGRPIVNMFPLQEGEKI
                     NVVLPLTGEMRSFPEDRYIFMATSMGTVKKTALDEFSNPRKAGIIAVNLDDGDYLVGA
                     ALTDGQHDVMLFSDGGKAVRFDENDVRPLGRQARGVRGMMLEDGQSVIAMLVAEDEEQ
                     SVLTATENGYGKRTSIVEYTRHGRGTKGMIAIQQSERNGKVVAATLVHADDEIMLITD
                     TGVLVRTRVSEIRELGRATQGVTLISLDEGAKLSGLQRIVENDANAAAEAHDTDAPPP
                     VDA"
BASE COUNT          531 a         1119 c         1149 g          721 t
ORIGIN      
        1 atggcgttgt agatgctggc gcgcatgccg cccacggact tgtggccctt gagctgcagc
       61 aggccgcgcg ccttggcgcc ggccaggaag gcgtcgttgc ggctttcgtc ctgtaggaag
      121 aacgggatgt tcatgcgcga gcggcagttg gcggccacct tgttcacata gaagggcgag
      181 gcgtcgatga agtcgtacag cagccgcgcc ttggcaatgt tgcgcgcctc catggccgct
      241 acgccgctca aggccccttc tcgctgcttc ttgagccatt ggaaggtcag gcccgccatg
      301 tagatgcccc aggtcggcgg cgtgttgtac atggagtggt tgtcggccac gacacgatag
      361 tcaaaggcgc tgggacaggc gctcaatgca tggcccagca ggtcgtcacg cactatgacc
      421 atggtcagcc cggccgggcc gatgttcttt tgcgcgccgc caaaggccag gccgacgcgg
      481 ctccaatcga cggggcgcga ggcgacatgg gaggaaaagt cgatcaccag cggcgcttcg
      541 ctgcccaggc ttttcaggtc gggcagctcg tggaattcga cgccgtggat ggtctcgttg
      601 ctgcagatat gcacgtagct ggcgccacgg ctgagctgcc agcccgccgg gtcgggcagg
      661 gtggtgaagc cgctggcctc gcccgaggca gcggtgtgta cctcggccgc gtacttggcg
      721 gcttccttgc gcgacttctg gctccagctg ccggtgacca cgaagtccac ggtggccgcg
      781 cgcgagaggt tcagtggcac gatggcgttt tcggccaggc cgccgccctg catgaacaga
      841 atcttgaact ccggcggcac ggccagcagc tcgcgcaggt cggcttcggc ctgttcgtag
      901 atgctgatga actccttgcc gcggtggctc atctccatca cgcccatgcc gctgccatgc
      961 cagtccagca tctcgctggc ggcgcgttgc agcacctctt cgggaatggc ggccgggccg
     1021 gccgagaagt tgtaggggcg attcatggct atcaatcaaa tatgcatcta gcgcttatgt
     1081 gacaagcgca agcagctatt atttttataa tactacgcgt ccactggagg tggtgcgtcc
     1141 gtgtcgtgcg cctcggctgc ggcattggcg tcgttctcca cgatgcgctg caggccgctg
     1201 agcttggcac cttcgtccag cgagatcagt gtcacgccct gcgtcgcgcg gccgagctcg
     1261 cggatctcgg acacacgcgt gcgcaccagc acgccggtgt cggtgatcag catgatctcg
     1321 tcgtcggcat gcaccagggt ggcggccacg accttgccgt tgcgctcgct ttgctggatg
     1381 gcgatcatgc ccttggtccc gcggccgtgg cgcgtgtact cgacgatgct ggtgcgcttg
     1441 ccgtagccgt tttcggtggc ggtgagcacg ctttgctctt cgtcctcggc caccagcatg
     1501 gcgatcacgc tctggccgtc ctccagcatc atgccgcgca cgccgcgcgc ctggcggccc
     1561 agcgggcgca cgtcgttttc gtcaaagcgc acggccttgc cgccgtcact gaacagcatc
     1621 acgtcgtgct ggccgtcggt cagcgccgcg cctacaagat agtcgccatc gtccaggttc
     1681 acggcaatga tgccggcctt gcgcgggttg gaaaattcgt cgagcgccgt cttcttcacc
     1741 gtgcccatgc tggtggccat gaagatatag cggtcttcgg ggaagctgcg catttcgccg
     1801 gtgagcggca gcacgacgtt gattttttcg ccctcctgca gcgggaacat gttgacgatg
     1861 ggccgcccgc gcgagccgcg cgagccggca ggcacctccc acaccttgag ccagtacagc
     1921 cggcctcggt tggagaaaca caggatgtag tcatgcgtgt tggcgatgaa gagctggtcg
     1981 atccagtcgt cctctttggt ggccgcagcc tgcttgccgc gtccgccgcg cttttgcgcg
     2041 cggtactccg ataggggctg gctcttgata tagccggtat gcgacagcgt caccaccatg
     2101 tcggtgggcg tgatcaggtc ttcggtggac aggtcttgcg cgctgtgctc gatctcgcta
     2161 cggcgcgcgc ccagcttggt ttgaccgaac tcctggcgca cggcggacag ctcttcaccg
     2221 atgatggtgg acacgcgtgc gggcttggcc agaatgtcca gcaagtcctc gatgaccgcc
     2281 atgatgtcct tgtactcggc gacgatcttg tcctgctcca ggccggtcag gcgctgcagg
     2341 cgcatctgca aaatctcttg tgcctgggtg tccgacaggc ggtacaggcc gccgtcctgc
     2401 atgccgtatt cgcgctccag gccctcgggg cggtagtcgt cggcgctcac cacgccgccg
     2461 tcctcgcgcg tgcgcgtcag catctcgcgc accagctggc tgtcccagct gcgggccatc
     2521 agctcggcct tggccaccgg tggcgtcggg gcattgcgga tgatgcggat gaactcgtcg
     2581 atattggcta gcgccacggc caggccctcg agcacatggc cgcgttcgcg tgccttgcgc
     2641 agctcgaaca cggtgcggcg cgtcacgacc tcgcggcggt ggccgatgaa gacctcgatc
     2701 aagtccttca gattgcacag cctgggctgg ccgtcgacca gcgccaccat gttcatgccg
     2761 aaggtgtcct gcaactgcgt ttgcttgtac aggttgttga gcaccacctc gggcacttcg
     2821 ccgcgcttca attcaatcac caggcgcatg ccggacttgt ccgactcatc ctggatatgg
     2881 ctgatgccct cgatcttctt gtcgttgacc agctcggcca tgcgctcctg cagcgtcttt
     2941 ttgttcacct ggtagggcag ctcatcgacg atgatggcct ggcgctggcc gcggtctatg
     3001 tcctcgaagt gcaccttggc gcgcatcacc acgcgcccgc gcccggtgcg gtagccgtcc
     3061 ttgacgccct ggatgccgta gatgatgccg gccgtgggga agtcgggcgc ggggatgatc
     3121 tccatcagct catcaatcgt tgcctcgggg tggcgcagca agtgcaggca ggcatcgacg
     3181 acttcgttaa ggttgtgcgg cggaatgttc gtcgccatgc ccacggcaat gccggcgctg
     3241 ccgttgacca gcaggttcgg caggcggctg ggcagcacca gcggttcttt ctcgctgcca
     3301 tcgtagttgg gcccgaagtc caccgtctcc ttgtcgatgt cggccagcat ctcgtgggcg
     3361 atcttcgaca ggcggatttc cgtgtagcgc atcgcggcgg cgttgtcgcc atcgaccgag
     3421 ccgaagttgc cctggccgtc gaccagcatg tggcgcagag aaaaatcctg cgccatgcgc
     3481 acgatggtgt cgtacaccgc gatgtcgccg tgcgggtggt
//
LOCUS       DAOMTQ010000236         3401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7800_length_3401_cov_24.112373, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000236 DAOMTQ010000000
VERSION     DAOMTQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7800_length_3401_cov_24.112373"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2603)
                     /locus_tag="PK518_15100"
     CDS             complement(<1..2603)
                     /locus_tag="PK518_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HRM50287.1"
                     /translation="MARTAAPRFRKTMLALAAGAVVAPCSAWALNLAEAPPGTVEPYV
                     RPNVIISVDDSGSMDWQVTNSNTGDAAITTPNADGSWPNNAKRINILKYALTSIFDPT
                     HPEYDPALLPDKKIRLAWQTMWNNGNSPDAKNVDSTNMRQNSMRILEGSHRSNFLSFV
                     SSIKASNGTPSHWMFSQADAYMRRPLTVNSPWASDPGTTDTPYLACRRSYHIMMTDGR
                     WNGTALPVPDTNKRDNATNLPLPDGMVYGGASAADIAKTALYRDNDGWTSLADWAFYS
                     WAVPMQTGGMTGTMQTPADYNKAPATENFGKDSANKDAILERYWNPRYNPARWPHMVT
                     YTIGFSNDATTWPGASNIFAPAASDRLPFKFTGSFQDLVTGTKTWPPMDAESKRSLDL
                     WHAAINGRGRFYAVTKGEDLEQAFRDIFGQINTATDPNLSASATSGSNVSRSEVGKYT
                     AGYEPDKFWKGTITADKVKKDGTTYQDPNWNGKSTADLLDAATVGSRLVLSWSDKWTG
                     TAFQGGVPFQWAADESKLSTNQKLWLQKNSSNVDEGATKGQQRLDYIRGDRSLEGVDP
                     GGYSGSKPFRQRMSIQGDIINSDVWYTGAPAANHMLKGYTAFVKYHKSRPGMLYVGGN
                     DGMLHGFAAYDGQEKLAYVPRGVIPTLTLLTDPQYNNKHKYFVDGSPMTGDVDLNGGM
                     QDPNDPNYGIYTANWRTLLVGTLGLGGKGYFVLDVTNPSANNLGNGTPGMSETNAAQL
                     VKLDRTRGAGESAPNCAVLTGAEQAACLQIVDEDKDIGHITARPVRDDNDSMRTTQIT
                     RMNNNRWAVVLGNGYNSANQRPVLLVQYLDGAKELLRLPVTTDSAGTGKAADNGLASP
                     RVVDLNGDGLT"
     gene            complement(2694..>3401)
                     /locus_tag="PK518_15105"
     CDS             complement(2694..>3401)
                     /locus_tag="PK518_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRM50288.1"
                     /translation="FVMLSMLLALWASRTSLFNELVVGNDADYQRAFEAAQALLQDAE
                     LDIRDERPDGTACNAAGCRDMGSAKKIPLSAQEVTPLLADLDAQPTKCQDGLCVKRTG
                     RQDFWNYTSATSPAPTNLQADEFKLEDMTKANVGARYGDYTGATAGSASSPANPILAD
                     RSADNRGGWYWIEIMNYDESSKNSGLIVGGATTDNLLRLNLTPNVVYRITALAYGRKP
                     GTMAVLQQTYARQIRKD"
BASE COUNT          513 a          939 c         1151 g          798 t
ORIGIN      
        1 tcggtaaggc catcgccgtt gaggtccacc acacgcggcg aggccaggcc gttgtccgcg
       61 gccttgccgg taccggcact gtcggtggta acgggcaggc gcagcaactc cttggcgccg
      121 tcgaggtact gcaccaacag caccgggcgc tggttggcgc tgttgtagcc attgcccagc
      181 accacggccc agcggttgtt gttcatgcgc gtgatttgcg tggtgcgcat gctgtcgttg
      241 tcgtcccgca cggggcgggc cgttatgtgg ccgatgtcct tgtcttcatc tactatttga
      301 aggcaggcgg cttgttccgc gcccgtgagg acggcgcaat tgggcgcaga ttcgccggct
      361 ccgcgcgtgc gatcgagttt gaccagttgc gcggcattgg tctctgacat ccccggggtt
      421 ccgttgccca gattgttggc gcttgggttg gtgacatcca gcacaaagta acccttgccg
      481 cccaggccca gagtgcctac cagcagcgtg cgccagttgg cggtgtagat gccgtaattg
      541 ggatcgttgg gatcctgcat gccgccgttg aggtctacgt cgcccgtcat gggtgaacca
      601 tcgacgaaat acttgtgctt gttgttgtac tgcggatctg tcagcaacgt gagtgtggga
      661 atgaccccgc gcggcacata ggcgagtttt tcttgtccgt cgtacgctgc aaatccgtgc
      721 agcatgccgt cgttgccacc gacatacagc atgcctgggc gggacttgtg gtacttcacg
      781 aaggctgtgt agcctttgag catatggttg gccgcaggtg ccccggtgta ccacacgtcg
      841 gagttgatga tgtcgccctg gatgctcatg cgctggcgga agggcttgga tccgctatag
      901 cccccggggt ccacaccctc cagggatctg tcaccccgga tgtagtccag ccgctgctgg
      961 cctttggtgg cgccctcatc gacgttgctg ctgtttttct gtagccacag cttttggttg
     1021 gtgctgagct tgctttcgtc tgcggcccat tgaaagggaa ccccgccctg aaaggcggtt
     1081 ccagtccact tgtcgctcca gctcaacacc aggcgtgagc caacggttgc ggcgtcaagc
     1141 aggtctgcag tggacttgcc attccagttg gggtcttggt aggtggtgcc gtcctttttg
     1201 actttgtctg cggtgatggt gcccttccag aacttgtcgg gttcgtagcc cgcggtgtac
     1261 ttgccgactt cgctgcgcga gacgttggag ccgctggtgg cgctggcgct gaggttgggg
     1321 tcggtggcgg tattgatctg gccgaaaatg tcacggaagg cttgttccag atcctcgccc
     1381 ttggtcaccg cgtagaagcg gcccctgccg ttgatcgcgg cgtgccacaa atccagcgag
     1441 cgcttgcttt cggcatccat tgggggccag gttttcgtgc cagtcacaag atcctggaag
     1501 ctgcccgtga acttgaaggg caagcggtcg ctggcggccg gggcaaagat attggatgca
     1561 cccggccagg tggtggcatc gttactgaag ccgatggtgt aggtgaccat gtgcggccac
     1621 ctggcggggt tgtagcgcgg gttccagtag cgttcaagga tggcatcctt gttggcgctg
     1681 tccttgccga agttttcggt agcgggggct ttgttgtaat cggctggggt ttgcatggtg
     1741 ccggtcatgc cgccggtctg catggggact gcccagctgt agaacgccca atcggccaga
     1801 gaagtccacc catcgttgtc gcggtaaagc gctgtcttgg cgatgtccgc cgcgcttgcg
     1861 ccgccgtaca ccatgccgtc tggcagcggc aggttggtgg cgttgtcacg cttgttcgtg
     1921 tccgggactg gcagtgccgt accgttccaa cgcccgtcgg tcatcatgat gtgatagctg
     1981 cgcctgcacg caaggtacgg cgtgtctgta gtgcctgggt cgcttgccca ggggctgttg
     2041 accgtgagtg gccggcgcat ataggcatcg gcctggctga acatccagtg cgaaggggtg
     2101 ccgttgcttg ccttgatgct gctgacaaag ctcagaaaat tgctgcgatg gctgccctcc
     2161 aggatgcgca tggagttttg ccgcatgttt gtgctgtcaa cgtttttcgc atccggggag
     2221 ttgccgttgt tccacatggt ttgccaggcc aggcgtatct ttttgtctgg cagcagggct
     2281 gggtcgtact ctgggtgtgt cgggtcaaaa atcgacgtga gcgcgtattt caatatgttg
     2341 atgcgcttcg cgttgttggg ccagcttccg tcagcattgg gggtggtgat ggctgcgtcg
     2401 ccggtgtttg agtttgtaac ctgccagtcc atgctccccg agtcatccac ggagatgatg
     2461 acattcgggc gcacataggg ttcgaccgtt ccaggcggcg cctcggccag gttgagtgcc
     2521 caggcactgc agggtgcaac aacggcgccg gccgccaggg cgagcatggt tttgcgaaaa
     2581 cgaggggcag cggtgcgggc catggctggc tccttggttc aatgggttaa tggcaaaaat
     2641 gtggcagtgg cgtgcttcga tcaatcacga cttgctacat aagcggtagc ggttcagtct
     2701 ttgcgtattt gccgggcgta ggtttgctgc agcacggcca tggtgccggg cttgcgccca
     2761 taggccaggg cggtgatgcg gtacaccacg ttgggcgtca ggttcagacg cagcaggttg
     2821 tcggtggtgg cgccgccaac gatcaggcca gagttcttgc tgctttcgtc gtagttcatg
     2881 atttcgatcc agtaccagcc gccacggttg tcggcgctgc ggtcggccag gatggggttg
     2941 gcggggctgc tggcactgcc tgcggtggcg ccggtgtagt cgccgtagcg ggcgccgacg
     3001 ttggccttgg tcatgtcttc cagcttgaat tcatcggctt gtagattggt gggggcggga
     3061 ctggtggcgc tggtgtagtt ccagaagtcc tgtcggccgg tgcgcttgac gcacagaccg
     3121 tcctggcact tggtgggctg tgcgtccagg tcggccagca ggggggtgac ctcctgggcg
     3181 gacagcggga ttttctttgc gctgcccatg tcccggcatc cggcggcgtt gcacgcggtg
     3241 ccatctggac gctcgtcgcg aatgtcgagt tctgcatcct gcagcagtgc ctgggcggct
     3301 tcgaaggcgc gctggtagtc ggcatcgttg cccaccacca actcattgaa cagcgaggtg
     3361 cgcgaagccc acagcgccag cagcatggac agcatcacga a
//
LOCUS       DAOMTQ010000237         3370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_7918_length_3370_cov_21.677225, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000237 DAOMTQ010000000
VERSION     DAOMTQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7918_length_3370_cov_21.677225"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PK518_15110"
     CDS             <1..146
                     /locus_tag="PK518_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRM50289.1"
                     /translation="ARDLGYTFVSHQQEVGAGYFDDVTTVIQGGSSSVKALTGSTEEE
                     QFH"
     gene            complement(269..1135)
                     /locus_tag="PK518_15115"
     CDS             complement(269..1135)
                     /locus_tag="PK518_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRM50290.1"
                     /translation="MNHQDTVFACIDGLATTHAVVDGAFWAAERLQAPMALLHALERP
                     EPMPPVGDYSGVIGMGAQDMLLERLNTLDEERTHVAQEAARRMLDEAQARVDQTRIPS
                     LQVLLRHGELTDVLLEHEDGARLFVLGRQHRATAARKLRLDHRVESVIRNVRQPVMVM
                     TTPEFTPPEGFVVAFDGSTTARRAVEAVARSPLLRGMPGLLAMAGEPTPEMLEQLHGA
                     QAQLQQAGFAIDTRVVPGLPEEALPALLQSLGNMLLVLGAYGHSRIRQLIVGSTTTAL
                     LRLCSVPVVVLR"
     gene            complement(1285..2190)
                     /locus_tag="PK518_15120"
     CDS             complement(1285..2190)
                     /locus_tag="PK518_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM50291.1"
                     /translation="MNISSRQIDAFLALAAQRNFTRAAAQCHLSQPAFSALIRALEDA
                     LGLRLFDRSTRHVALTAEGENFHASALRIRAEVDAALAGMRDAAQLRRGRVAIALLPS
                     LAAGWLPGVLADFHARHPGVELDIADVLSEPCMERVASGRADFALAAIRADTPELQAE
                     PFCQDGFHLVCRADHPLATRTLPAGPAQLRVLAEWPFIHLARHSSVRQYLEAAFHPQA
                     MNTLMEVEQLATVMGMVRAGLGISVVPSLTLFHFQQPDLLTRPLALPGLRRQIYLVRR
                     RDRSLSVAAQALYQLMLLQRPQDAA"
     gene            2284..>3370
                     /locus_tag="PK518_15125"
     CDS             2284..>3370
                     /locus_tag="PK518_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HRM50292.1"
                     /translation="MANHSPLLVGCAAGFSGDRTDAAAPVVQALVDSGRPAVLIFETL
                     AERTLALAQLARRGNPEAGYEPLLDDLLRPVLAQCLRHGVRIVSNFGAANPHGAALRI
                     QQLARELGARRPRVAVVHGDDLSGPEHRALLAQALGADMPTQPIVSANAYIGAQPIAD
                     ALRAGADIVVCGRVADPSLVVGPALAHYGWQLDDWDRLARATMAGHLLECGAQVTGGY
                     FADPGYKDVAGLARLGYPIAEIDADGHCSITKPAGTGGRVDERTVKEQLLYELHDPAA
                     YLTPDVVADITGAHVAQAGTDRVRLDGVRGHARTPTLKVNVCFESGWFAEGEISYAGA
                     RAEARARLAGDVLRERLAGVAPLRIDLI"
BASE COUNT          527 a         1171 c         1174 g          498 t
ORIGIN      
        1 ccgcacgcga cctgggctac accttcgtgt cgcaccagca ggaagtgggc gcgggttact
       61 tcgacgacgt gaccaccgtg atccagggtg gctcgtccag cgtcaaggcg ctgaccggct
      121 ccaccgaaga agagcagttc cactgatctg tcggccgacg gccggactga aacaacaaag
      181 cgcccgcgag ggcgctttgt tgtttatgga taaaaaatgg ctttagcgct tatgtatcaa
      241 gcgcataaag ctattgtttt gatagtaatc agcgcaacac caccaccggc accgagcaca
      301 gccgcagcag cgccgtggtg gtgctgccca cgatcagctg gcggatgcgc gaatgcccgt
      361 aggcgcccag caccagcagc atgttgccca gcgattgcag cagggccggc agggcctctt
      421 cgggcaggcc gggcacgacc cgggtgtcga tggcaaagcc tgcctgctgc agctgcgcct
      481 gggcgccgtg cagctgctcc agcatctccg gcgtgggctc gccggccatg gccagcaggc
      541 cgggcatgcc gcgcagcagc ggactgcgcg ccacggcctc cacggcgcgg cgggcggtgg
      601 tgctgccgtc gaaggcgacg acgaagccct cgggcggcgt gaactcgggc gtggtcatca
      661 ccatcaccgg ctggcgcacg ttgcggatca cgctttccac gcggtgatcc aggcgcagct
      721 tgcgcgccgc cgtggcgcgg tgctggcgcc cgagcacgaa caggcgcgcg ccatcctcgt
      781 gctccagcag cacatccgtc agctcgccat ggcgcagcag cacctgcagc gacggtatgc
      841 gcgtctgatc cacgcgcgcc tgggcctcgt ccagcatgcg ccgagcggcc tcctgcgcga
      901 catgggtgcg ctcttcgtcc agggtgttca ggcgctcgag cagcatgtcc tgcgcgccca
      961 tgccgatgac gccgctgtag tcgcccaccg gcggcatggg ctcgggccgc tccagcgcgt
     1021 gcagcagggc catcggggcc tgcaggcgct ccgccgccca gaaggcgcca tccaccacgg
     1081 catgggtggt ggccagcccg tcgatgcagg cgaaaacggt gtcttggtgg ttcatggggc
     1141 tccttcctgg cgacctgacc atgggttgag tgtggcaccg tggcgcaggc tgtgccaatc
     1201 cactctccaa tgtggggttg gttttaggtg aaatatgcca tcaacgcaat acaggcgtgc
     1261 gctataagct atagtttttg tagttcatgc tgcatcttgt ggccgttgca gcagcatcag
     1321 ctggtacagc gcctgcgccg ccaccgacag gctgcggtcg cgccggcgca ccaggtagat
     1381 ctgccgcctc aggccgggca gggccagcgg ccgcgtgagc agatcgggct gctggaagtg
     1441 aaacagcgtc aggctgggca cgacgctgat gcccagtccg gcgcgcacca tgcccatcac
     1501 ggtggccagc tgctccactt ccatcaaggt gttcatggcc tgggggtgga aggccgcctc
     1561 caggtactga cgcacgctgc tgtggcgtgc caggtgaatg aagggccatt cggccagcac
     1621 gcgcagctgc gccgggccgg cgggcagggt gcgggtggca agcgggtgat ccgcgcggca
     1681 gaccaggtga aaaccgtcct ggcagaaggg ttcggcctgc agttcgggcg tgtcggcgcg
     1741 aatggccgcc agggcaaagt cggcccggcc gctggccacg cgctccatgc agggctcgga
     1801 cagcacatcc gcaatgtcca gctccacgcc cgggtggcgc gcgtggaaat cggccagcac
     1861 ccccggcagc cagcccgcgg ccagcgaggg cagcagcgcg atggccacgc gcccgcgccg
     1921 cagctgggcc gcatcgcgca tgccggccag ggccgcgtcc acctcggcgc gtatgcgcag
     1981 tgcgctggcg tggaaattct cgccctcggc cgtcagagct acatggcgcg tgctgcggtc
     2041 aaacaggcgc aggcccaggg cgtcctccag cgcacggatg agcgcgctga aggccggctg
     2101 cgacaaatgg cactgcgccg ccgcgcgcgt gaagttgcgc tgcgcggcca gggccaggaa
     2161 ggcgtcgatc tggcggctgg agatattcat ttgaattacc gatgaattga tcttgacaat
     2221 ctatttcaca gaataacccg cgccgcctac agtgcacgcc agagacaacg cgaaggacag
     2281 gccatggcga atcactcccc cctgctggta ggctgcgcgg ccggtttttc cggcgaccgc
     2341 accgacgccg ccgcgcccgt ggtgcaggcc ctggtggaca gcggccggcc cgcggtgctg
     2401 atcttcgaga ccctggccga gcgcacgctg gcgctggccc agctcgcccg ccgcggcaac
     2461 cccgaagccg gctacgaacc gctgctggac gacctgctgc gccccgtgct ggcgcaatgt
     2521 ctgcgccatg gcgtgcgcat cgtgagcaac tttggcgcgg ccaacccgca tggcgcggcg
     2581 ctgcgcattc agcagctggc gcgggagctg ggcgcgcgcc gcccgcgcgt ggccgtggtg
     2641 catggcgacg acctgagcgg ccccgagcac cgcgccctgc tggcgcaggc cctgggcgcc
     2701 gacatgccca cccagcccat cgtcagcgcc aacgcctaca taggcgcgca gcccattgcc
     2761 gacgcgctgc gcgcgggcgc cgacatcgtg gtctgcggcc gcgtggccga cccgtccctg
     2821 gtggtcggcc cggcgctggc gcactacggc tggcagctgg acgactggga ccgcctggcg
     2881 cgcgccacca tggccggcca tctgctcgaa tgcggcgccc aggtcacggg cggctacttt
     2941 gccgaccccg gctacaagga cgtggcaggc ctggcgcgcc tgggctaccc cattgccgag
     3001 atcgacgccg acggccactg cagcatcacc aagccggccg gcacgggcgg gcgcgtggac
     3061 gagcgcaccg tcaaggagca gctgctgtat gagctgcacg acccggccgc ctacctgacg
     3121 cccgacgtgg tggccgacat caccggcgcc catgtggcgc aggccggcac cgaccgcgtg
     3181 cgcctggacg gcgtgcgcgg ccatgcgcgc acgcccacgc tcaaggtgaa tgtgtgcttc
     3241 gagtccggct ggttcgccga gggcgaaatc tcctacgccg gcgcccgcgc cgaggcccgc
     3301 gcccgcctgg ccggcgacgt gctgcgcgag cgcctggcgg gcgtggcgcc gctgcgcatc
     3361 gacctgatag
//
LOCUS       DAOMTQ010000238         3322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_8102_length_3322_cov_30.811754, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000238 DAOMTQ010000000
VERSION     DAOMTQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8102_length_3322_cov_30.811754"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..260)
                     /locus_tag="PK518_15130"
     CDS             complement(9..260)
                     /locus_tag="PK518_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50293.1"
                     /translation="MSKTSFPLRIQNDERERAKRLAEALGVSENRLYAELIHDGMLIR
                     EQMLYMGQLRALAAGTPLQDALAVLDKVPATPPLETDRV"
     gene            complement(283..708)
                     /locus_tag="PK518_15135"
     CDS             complement(283..708)
                     /locus_tag="PK518_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRM50294.1"
                     /translation="MRYLLDTNVLVAALRSSQGASHALVRRALQGELPLVVHQKLVYE
                     YRDVLSRPTTLADTGLAWADAELVLAHLVARAQEVHVRYLWRPNLRDEGDNFVLEIAV
                     AAWPCTLVTHNLADFARGQLRFPQIPITTPGQLLRSLTH"
     gene            complement(771..1562)
                     /locus_tag="PK518_15140"
     CDS             complement(771..1562)
                     /locus_tag="PK518_15140"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HRM50295.1"
                     /translation="MEPLNEFFSWNLRHINAEGERLMTEDELLELFERMEADDLPETA
                     MSMGEADGYMTALVIAPDPVMLHQWMEAIFGQPTLPLAEDPAAQTRLLELLRARYLDI
                     QLRLATPRSQITTDNLFTPLHADVDPGDCITPYQLDADGLRRGDWTLRDWAQGFRTAV
                     SEDLSWDELIEDPDDYHLISPIVLFDLGHNPDRPEQQIDQDDAFAQLLITTLYELMAW
                     WRDPHRRAVREQLARMPLLRQSVKVGRNDPCPCGSGKKYKKCCGA"
     gene            complement(1576..1797)
                     /locus_tag="PK518_15145"
     CDS             complement(1576..1797)
                     /locus_tag="PK518_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRM50296.1"
                     /translation="MMGIEWLRPAAFLGSILYALIGVVIFWLCFVLIDKITPTDLWAE
                     IVEKQNRALALVVAAMCLGISIIVAAAIH"
     gene            complement(1835..>3322)
                     /locus_tag="PK518_15150"
     CDS             complement(1835..>3322)
                     /locus_tag="PK518_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HRM50297.1"
                     /translation="SAPQRSYRAPCPGCGAPVEFRSAQSTHAICAYCQSMVVRSGEVL
                     QRLGKMAELFDDHSPLRLMASGRIEQDGKDQPFTLIGRLQYQGEQGRWTEWIAFLQDG
                     GSGVLSEDNGAYVFTRPARLGRDAPAPEQLRVGMTTAVGGQPFSVAANMQVRLVSAQG
                     ELPKLPPLGQPFQVVELRSADGEVLSIDYGSQPPQVQRGRAVRLQDLRLVGIKDESAR
                     DEQGRQFNCPRCGAPVRVELASSKSCTCPACDSIIDLSAGLGGELRHAAQYEPVQPLI
                     ALGSVGQFEGAPWQVVGFQHRSGREAGDDENFGWEEYLLYNRQRGFIFLVDASDGWSL
                     VRPTTGAPQYKPGAQQASYLGRQYRLTSSYQAETTYVAGEFYWPVVRGQKTGNSDFAA
                     VNGRGILSREQTPGEITWSHGSRIDSSAVAQAFKLGDKAGLFRRQDAGPVAAASGIGC
                     GTLILVLFILFVLLVLMSNCSGSPGGGVRSSGGSFGGYSTGGGHK"
BASE COUNT          506 a         1084 c         1172 g          560 t
ORIGIN      
        1 gcggcgcttc aaaccctgtc cgtctccagg ggcggggtgg cgggcacctt gtccagcacc
       61 gccagcgcat cctgaagcgg cgtgccggcc gccagcgcgc gcagctggcc catgtagagc
      121 atttgctcgc ggatcagcat gccgtcgtgg atcagctcgg cgtagaggcg gttctccgac
      181 acgcccaggg cctcggccag gcgcttggcg cgctcgcgct cgtcgttctg gatgcgcagg
      241 ggaaaggatg ttttgctcat catggcctcc ggtggtggcg ggtcaatgcg tcaggctgcg
      301 cagcagctgc ccgggtgtgg tgatggggat ctgcgggaag cgcagctgac cccgggcaaa
      361 gtcagccagg ttgtgcgtga ccagggtgca gggccaggcg gcaaccgcga tttccagcac
      421 gaagttgtcg ccctcgtcgc gcagattggg gcgccagagg tagcgcacat gcacctcctg
      481 ggcacgtgcc acgaggtgcg ccagcaccag ctcggcgtcg gcccaggcaa ggccggtatc
      541 ggccagcgtg gtgggtcggc tcaacacatc gcggtattcg tacaccagct tctggtgcac
      601 cacgagcggc agctcacctt gcaaggcgcg gcgcaccagg gcgtggctgg caccttggct
      661 ggaacgcaag gcagccacca gcacgttggt gtcaagcaga tatcgcatgg atagatgata
      721 tctaaagtga tgtcatttat cccctgtggt ttatttcccc acgggacaag tcacgccccg
      781 cagcatttct tgtacttctt gccactgccg caggggcagg ggtcgttgcg gccgaccttg
      841 accgattggc gcagcagcgg catacgggcc agctgctccc gcacggcccg gcggtggggg
      901 tcgcgccacc aggccatcag ctcatacagg gtggtgatca gcagctgcgc aaatgcgtcg
      961 tcctggtcga tctgctgctc gggccggtcg gggttgtggc ccaggtcgaa gagcacgatg
     1021 gggctgatca ggtggtagtc gtcggggtct tcgatcagct cgtcccagct caagtcctcg
     1081 gacacggcgg tgcgaaagcc ctgggcccag tccctgagcg tccagtcgcc ccggcgcagg
     1141 ccgtccgcgt cgagctggta gggcgtgatg cagtcgccgg gatcgacgtc ggcgtgcagc
     1201 ggcgtgaaca ggttgtcggt ggtgatctgg ctgcgcggcg tggccaggcg cagctggatg
     1261 tcgaggtagc gcgcgcgcag cagttccagc agccgcgtct gggcggcggg gtcttcggcc
     1321 agcggcaggg tgggctggcc gaagatggcc tccatccact ggtgcagcat caccgggtcg
     1381 ggggcgatga ccaacgcggt catgtagccg tcggcctcgc ccatgctcat ggcggtctcg
     1441 ggcaggtcgt cggcctccat gcgctcgaag agctccagca gctcgtcctc ggtcatcagg
     1501 cgctcgccct cggcgttgat atggcgcagg ttccaggaga aaaattcgtt cagcggttcc
     1561 atgggcacag gcctttcagt ggatggcggc ggcgacgatg atgctgatcc ccaggcacat
     1621 ggccgccacc accagcgcca gggcgcggtt ttgtttttcg acgatctcgg cccacaggtc
     1681 ggtgggcgtg atcttgtcga tgagcacaaa gcacagccag aagatgacca cgccgatcag
     1741 cgcgtagagg atggacccga gaaaggccgc gggccgtagc cattcgattc ccatcatggc
     1801 gtttgctcct tgtagggttg ggtgaaagaa cgattcattt gtggccgccg ccggtgctgt
     1861 agccgccgaa cgagccgccc gagctgcgca cgcccccgcc cgggctgccc gagcagttgc
     1921 tcatcagcac cagcagcacg aagaggatga acaacaccag gatcagcgtg ccacagccta
     1981 tgccggaggc ggcagcgacc ggccccgcgt cctggcgccg gaacagcccg gccttgtcgc
     2041 cgagcttgaa ggcctgggcc acggcgctgc tgtcgatgcg gctgccgtgc gaccaggtga
     2101 tctcgcccgg tgtctgctcg cgcgagagga tgccgcggcc gttcaccgcg gcaaagtcgc
     2161 tgttgccggt cttctggccg cgcaccacgg gccagtagaa ctcgccggcg acataggtcg
     2221 tctcggcctg gtagctggag gtcaggcggt actgccggcc caggtagctg gcctgctgcg
     2281 cgcccggttt gtactgcggc gcgccggtgg tggggcgcac caggctccag ccgtcgctgg
     2341 cgtccaccag aaagatgaag ccgcgctggc ggttgtagag caggtattcc tcccagccaa
     2401 agttctcatc gtcgccggct tcgcgcccgg agcgatgctg aaaaccgacc acctgccagg
     2461 gcgcgccctc gaactggccc acgctgccca gggcgatcag cggctgcacg ggttcgtact
     2521 gggcggcgtg gcgcagctcg ccccctaggc cggcgctcag gtcgatgatg ctgtcgcacg
     2581 ccgggcaggt gcagcttttg ctgctggcca gctccacgcg caccggtgcg ccgcagcgcg
     2641 ggcagttgaa ctggcggccc tgctcgtcgc gggcggattc gtccttgatg ccgacaaggc
     2701 gcaggtcttg cagccgcacg gcgcgcccac gctgtacctg gggtggctgg ctgccgtagt
     2761 cgatggacag cacctcgcca tcggcgctgc gcagctccac cacctggaag ggctggccca
     2821 gcggcggcag cttgggcaac tcgccctggg ccgagaccag gcgcacctgc atgttggccg
     2881 ccacgctgaa gggctggccg cccacggccg tggtcatgcc cacgcgcagc tgctcgggcg
     2941 ccggggcgtc gcggcccagc cgggccgggc gggtgaagac gtaggcgccg ttgtcctcgc
     3001 tcaacacacc gctgccgccg tcttgcaaaa acgcgatcca ctccgtccag cggccctgct
     3061 cgccctggta ctgcaggcgg ccgatcaggg tgaagggctg atccttgcca tcctgctcga
     3121 tacgcccgct ggccatgagg cgcagcgggc tgtggtcgtc gaacagctcg gccatcttgc
     3181 ccaggcgttg cagcacctcg ccgctgcgca ccaccatgct ctggcagtag gcgcagatgg
     3241 cgtgcgtgga ctgggcgcta cggaattcga ccggcgcgcc gcagccgggg cagggcgcgc
     3301 ggtagctgcg ctggggggcg ct
//
LOCUS       DAOMTQ010000239         3287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_8233_length_3287_cov_28.722463, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000239 DAOMTQ010000000
VERSION     DAOMTQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8233_length_3287_cov_28.722463"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK518_15155"
     CDS             complement(<1..357)
                     /locus_tag="PK518_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM50298.1"
                     /translation="MPSNPSTAPRLPLEGLRVVEFTHMVMGPTCGMVLADLGAEVIKV
                     EPVEGDRTRHLLGAGAGFFPMFNRNKKSIAIDLRKRQGREVAIRLASSADVVVQNFKP
                     GVMAKYGLDYAALSRLN"
     gene            613..918
                     /locus_tag="PK518_15160"
     CDS             613..918
                     /locus_tag="PK518_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRM50299.1"
                     /translation="MSSKENAAVFQLFEKLECRYALRVLWALRDGHPQTFRLLQDSVG
                     GITPNTLNTRIKELREAGFMDHGSDGYIVTPSGQDLLKRLSDVQAFANRWTAARAKK"
     gene            1034..1393
                     /locus_tag="PK518_15165"
     CDS             1034..1393
                     /locus_tag="PK518_15165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRM50300.1"
                     /translation="MAFTTTASGLQYEDSVVGTGAQATRGQSVRVHYTGWLYKDGQQG
                     AKFDSSRDRNDPFEFPLGAGMVIKGWDEGVQGMQIGGQRTLIIPPELGYGARGAGGVI
                     PPNATLKFDVELLAVKG"
     gene            complement(1465..>1707)
                     /gene="rimO"
                     /locus_tag="PK518_15170"
     CDS             complement(1465..>1707)
                     /gene="rimO"
                     /locus_tag="PK518_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837786.1"
                     /note="catalyzes the methylthiolation of an aspartic acid
                     residue of the S12 protein of the 30S ribosomal subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRM50301.1"
                     /translation="EVSTARLQRRVGQTLQVLVDKSVGLGKKGGVGRSWADAPEIDGL
                     VHLLPPEKISKTYKVGDFVKARIVGTQGHDLVGMPV"
     assembly_gap    1708..1807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1808..2502)
                     /locus_tag="PK518_15175"
     CDS             complement(<1808..2502)
                     /locus_tag="PK518_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM50302.1"
                     /translation="MSEALSPTKNPKIGFVSLGCPKNLTDSELILTQLSAEGYETSKT
                     FQGADLVIVNTCGFIDDAVRESLDTIGEALADNGKVIVTGCLGARADDGGGNLVKGVH
                     PNVLAVTGPHATQEVMEHVHQHLPKPHDPFIDLVPGAFSEAGIKLTPRHYAYLKISEG
                     CNHRCTFCIIPSMRGDLVSRPIGDVLKEARALFAGGVKELLVVSQDTSAYGVDVKYRT
                     GFWDGKPVRTRLL"
     gene            complement(2530..3069)
                     /gene="phaR"
                     /locus_tag="PK518_15180"
     CDS             complement(2530..3069)
                     /gene="phaR"
                     /locus_tag="PK518_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRM50303.1"
                     /translation="MSDNNTAPEAPTTQRVIKKYPNRRLYDTSTSTYVTLAEVKQLVM
                     NGESVAVRDAKSGEDLTRSILLQIILEEEAGGAPMFSEAALANIIRFYGHAMQGFMGA
                     YLEKNVQMFTEIQSKLAEQTQGFTPEMWSQFMNLQSPMMKGMMGNYMEQSQSMYQQMQ
                     EQMQKQTEQMLGAFGLKKR"
BASE COUNT          576 a         1027 c          973 g          611 t
ORIGIN      
        1 gttcaggcgc gagagcgcgg cgtagtccag gccgtacttg gccatcacgc ccggcttgaa
       61 gttctgcacc accacgtcag ccgagctcgc caggcggatc gccacctctc gcccctggcg
      121 cttgcgcagg tcgatggcga tgctcttctt gttgcggttg aacatcggga aaaaacccgc
      181 ccccgccccc agcaggtggc gcgtgcggtc gccctccacg ggctcgacct tgatgacctc
      241 ggcccccagg tcggccagta ccatgccgca ggtgggaccc atgaccatgt gggtgaactc
      301 caccacgcgc aggccttcaa gcggcaggcg gggtgcagtg gatggattcg aaggcatgtg
      361 gcaaagaccg atcaggagcc aagcgggaaa gccgcaaagc atgccatgct acgctgccgt
      421 cggccctgat ccaaccatca acactgcggc caatttatga cgtttgcgca cgacagatcg
      481 gcgccaggag ctaccaaact tgtagcggtt gcatttccgc gttatattgc tatcgttttt
      541 tgcgaccccg gtcaccccgc cgcatgcggc ggagaccccc atcacccaac ccaagcgtaa
      601 ggagaagcgt tcatgagctc caaggaaaac gccgccgtct ttcagctgtt cgagaaactc
      661 gaatgccgct acgcgctgcg cgtgctgtgg gcgctgcgcg acggccaccc gcagaccttc
      721 cgcctgctgc aggacagcgt tggcggcatc acgcccaaca cgctgaacac ccgcatcaag
      781 gaactgcgcg aagccggttt catggaccac ggcagcgacg gctacatcgt caccccctcg
      841 gggcaggatc tgctcaagcg cctgtccgac gtgcaggcct ttgccaaccg ctggacggca
      901 gcacgcgcga agaagtaaga cagccccttc acccacccaa cccgctcgat gcgggttttg
      961 gtctttttga gctcaagcgc ttgattggca agcgcagtaa gctctacttt ttgaagcaat
     1021 tcaaggaatc aacatggcat tcaccaccac ggcctccggc ctgcagtacg aagacagcgt
     1081 cgtcggcacc ggcgcccagg ccacacgcgg ccagtcggtg cgcgtgcact acaccggctg
     1141 gctctacaag gacggccagc agggcgccaa gttcgactcc agccgtgacc gtaacgatcc
     1201 gttcgagttt cccctgggcg cgggcatggt catcaagggc tgggacgagg gcgtgcaggg
     1261 catgcagatc ggcggccagc gcaccctcat catcccgccc gagctgggct atggcgcgcg
     1321 cggcgcgggc ggcgtgattc cgcccaacgc cacgctgaag ttcgacgtgg agctgctggc
     1381 cgtcaagggc tgaagccgcc ctgccccggc ccagaaaaaa aggagcgtgt gcaccacgct
     1441 cctttttttg tgccgacgat ggggtcagac cggcatgccc accaggtcat gcccctgcgt
     1501 gccgacgatg cgcgccttga cgaagtcgcc caccttgtag gtcttgctga tcttctcggg
     1561 cggcagcaag tggaccaggc catcgatctc gggcgcgtcg gcccagctgc ggcctacgcc
     1621 gcccttcttg cccaggccca cggacttgtc caccagcacc tgcagggtct ggcccacgcg
     1681 gcgctgcagg cgggcggtgg agacctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnntcc aacaggcgcg tgcgcaccgg cttgccgtcc caaaagcccg tgcggtattt
     1861 cacgtccacg ccgtaggccg aggtgtcctg gctgaccacc agcagctcct tcacgccgcc
     1921 cgcaaacagc gccctggcct ctttcagcac atcaccaatc gggcgcgaca ccaggtcgcc
     1981 acgcatcgag gggatgatgc agaaggtgca gcggtggttg cagccctcgg aaatcttcag
     2041 gtaggcgtag tggcgtggcg tgagcttgat gccggcctcg ctgaaggcgc cgggcaccag
     2101 gtcgatgaaa gggtcgtgcg gcttgggcaa gtgctggtgc acatgctcca tcacctcctg
     2161 cgtggcatgc gggccggtga cggccagcac attcgggtgc acgcccttaa ccaggttgcc
     2221 gccgccatca tcggcgcgcg cgcccaggca gccggtgacg atgaccttgc cgttgtcggc
     2281 cagggcctcg ccaatcgtgt ccaggctctc gcgcacggca tcgtcgatga agccgcaggt
     2341 gttgacgatg accagatccg cgccctggaa ggtcttggag gtctcgtagc cctcggcgga
     2401 gagctgggtg agtatgagtt cggagtccgt caggttcttg gggcagccca ggctgacgaa
     2461 gccgatcttg gggttttttg tcggggagag tgcttcgctc atccccgtat tttcccagtt
     2521 tagggaacgt cagcgttttt tgaggccaaa cgcacccagc atctgctcgg tctgcttctg
     2581 catctgctcc tgcatctgct ggtacatgct ttgcgactgc tccatgtagt tgcccatcat
     2641 gcccttcatc atgggtgact gcaggttcat gaactgcgac cacatctcgg gcgtgaagcc
     2701 ttgcgtctgc tcggccagct tggactggat ctcggtgaac atctgcacgt tcttctcgag
     2761 gtaggcaccc atgaagccct gcatggcatg gccatagaag cggatgatgt tggccagcgc
     2821 cgcctcgctg aacatgggcg cgccgccggc ctcttcttcc agaatgattt gcagcaagat
     2881 gctgcgcgtg aggtcttcgc cgctcttggc gtcgcgcacg gcgaccgact cgccattcat
     2941 caccagctgc ttgacctcgg ccagcgtcac ataggtcgag gtggaagtgt cgtacagccg
     3001 gcgattggga tacttcttga tcacgcgctg cgtggttggg gcttctggcg cggtattgtt
     3061 gtctgacact tacgactcct cgggtctctg gcccgtctgt attgcagtgc agcagattct
     3121 aggcaggcct ggcctgcatg gcggacagga ataccctcac tttgcatctg ccagctgatc
     3181 aaaattcagg caagaaaaaa gcaccaggca tttctgcgag gtgcttgatt caattggtag
     3241 gcgcgattgg actcgaacca acgaccccca ccatgtcaag gtggtgc
//
LOCUS       DAOMTQ010000240         3180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_8659_length_3180_cov_30.859840, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000240 DAOMTQ010000000
VERSION     DAOMTQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8659_length_3180_cov_30.859840"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK518_15185"
     CDS             complement(<1..316)
                     /locus_tag="PK518_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM50304.1"
                     /translation="MPPQPPHTHGPQSPDSTDPGADTPANASDIADSAEAAATAELQR
                     SAAARTPQSPLAPLSVPVFRMLWLTWLVANTCMWMNDVAAAWLMTTLTSSPVLVALVQ
                     TAS"
     gene            complement(337..822)
                     /locus_tag="PK518_15190"
     CDS             complement(337..822)
                     /locus_tag="PK518_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM50305.1"
                     /translation="METLDKYDLAILGALQRDARLTNAELAQRVGLSAAPCWRRVRAL
                     EEAGFIRGYRAEIDRHKIGLGVLAFVRLDAERSTGELTQAMERAIAQIPEVVSCHYIS
                     GTGTFELQVVARDLDSFSQFARGVLLNLPNVKDMHTSFSLGEVKAGGALPLAHLEQAR
                     A"
     gene            1005..>3180
                     /locus_tag="PK518_15195"
     CDS             1005..>3180
                     /locus_tag="PK518_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRM50306.1"
                     /translation="MNAPLPDHVRRALESVTLDDKYALGQGRAFMSGVQALVRLPMLQ
                     RQRDQAAGLNTAGFISGYRGSPLGGYDQALLGARKHLAESHVVFQPGVNEELGATAVW
                     GTQQLDIYPESKKYDGVFGIWYGKGPGVDRCSDVFKHANMAGTARHGGVIAVAGDDHI
                     SKSSTAAHQSDHIFKACGLPVFFPSSVQDILDMGLHAFAMSRFSGLWSGLKTVQEVVE
                     SSSSISVDPDRVNIILPEDFVMPPGGLHIRWPDAPLEQEARLMDYKWYAALAYVRANK
                     LNYNVVATPRDRFGLIASGKAYNDMRQALIDLGLDDGHCRQLGIRVHKVNVVWPLEAG
                     ITRDFAEGLQEILVVEEKRQVIEYQIKEQLYNWRADVRPDVLGKFDEVEGDHSGGEWG
                     MPNPSQNWLLRPQADLTPAIIARAIAKRLKKLGVPADIAARMDERLSVIEARERGMAE
                     QKLDTGERMPWFCSGCPHNTSTRVPEGSRAVAGIGCHYMVNWMPDRHTSTFTQMGGEG
                     VTWVGQAPFSKTPHVFANLGDGTYFHSGLLAIRQAIASGANITYKILYNDAVAMTGGQ
                     QVGERPEGHSVAQIAHSLRSEGVARLVVVTDEPEKYHGRTHSLAANPARAKHQELLND
                     LPPGVEVFHRDELDRIQRELRDIAGCTALIYDQTCATEKRRRRKRGTLAAPTKTVIIN
                     ELVCEGCGDCGVASNCLSVEPVETEFGRKRRINQSTCNKDYSC"
BASE COUNT          549 a         1067 c         1085 g          479 t
ORIGIN      
        1 tggaggccgt ctgcaccagc gccaccagca ccggcgagct ggtcagcgtg gtcatgagcc
       61 aggcggccgc cacgtcgttc atccacatgc aggtattggc caccagccag gtcagccaga
      121 gcatgcggaa cacgggcacg gacagcggcg ccaggggtga ctgcggcgtg cgcgcggcgg
      181 cgctgcgctg cagctcggca gtggctgcgg cctcggcgga gtcggcgatg tccgaggcgt
      241 tggcgggggt gtcggcgccg gggtctgtgc tgtcggggct ctggggcccg tgggtgtgcg
      301 gtggctgtgg cggcatgggc ccgccattat gaaacctcag gcccgggcct gctccagatg
      361 cgccagcggc agcgcgccgc cggccttgac ctcgcccagc gagaagctgg tgtgcatgtc
      421 cttcacgttg ggcaggttca gcagcacgcc gcgcgcgaac tgcgagaagc tgtccaggtc
      481 gcgcgccacc acctgcagct cgaaggtgcc ggtgccgctg atgtagtggc aggagacgac
      541 ctcggggatc tgcgcgatgg cccgctccat ggcctgcgtc agctcgccgg tgctgcgctc
      601 ggcgtccagg cgcacgaagg ccagcacgcc caggccgatc ttgtggcggt cgatctcggc
      661 gcggtagccg cggatgaagc ccgcttcctc cagcgcccgc acgcggcgcc agcagggcgc
      721 ggccgacagg cccacgcgct gcgccagctc ggcattggtc aggcgcgcgt cgcgttgcaa
      781 ggcgcccagt atggccaggt cgtatttgtc cagtgtttcc attttttgat tatttaaaga
      841 aagattcttt cttgcattgg ggtaaacgcg gcaaacaaag caaacacata tcagtaatca
      901 gcgcatacac tgcgcgccat gggccgggcc tgatcggcgc cggttcgttt gccgtgcccc
      961 cgcaacccgg ggcgcaagcc cgaagaacag cccggagaca ccccatgaat gcccccctgc
     1021 ccgaccatgt gcgccgcgcg ctcgagtccg tcaccctgga tgacaaatat gccctgggtc
     1081 agggccgcgc cttcatgagc ggcgtgcagg ccctggtgcg cctgcccatg ctgcagcgcc
     1141 agcgcgacca ggccgcgggc ctgaacacgg cgggcttcat cagtggctac cgtggctcgc
     1201 cgctgggcgg ctacgaccag gccctgctcg gcgcgcgcaa gcatctggca gagagccatg
     1261 tcgtgttcca gcccggcgtg aacgaggagc tcggcgccac ggccgtgtgg ggcacgcagc
     1321 agctcgacat ctaccccgag agcaaaaagt acgacggcgt gtttggcatc tggtatggca
     1381 aggggccggg cgtggatcgc tgctcggacg tgttcaagca tgccaacatg gccggcacgg
     1441 cgcgccacgg cggcgtgatc gccgtggccg gcgacgacca catcagcaag agcagcaccg
     1501 ccgcgcacca gagcgaccat atcttcaagg cctgcggcct gccggtgttc tttccgagca
     1561 gcgtgcagga catcctggac atgggtctgc atgcctttgc catgagccgc ttctcgggcc
     1621 tgtggtcggg cctgaaaacg gtgcaggagg tggtcgagtc gtcgagcagc atctcggtcg
     1681 atccggatcg tgtgaacatc atcctgcccg aggactttgt gatgccgccg ggcggcctgc
     1741 acatacgctg gcccgacgcg cccctggagc aggaggcgcg cctgatggac tacaagtggt
     1801 atgcggccct ggcctatgtg cgcgccaaca agctcaacta caacgtggtc gccacgcccc
     1861 gggatcgctt tggactcatc gccagtggca aggcctacaa cgacatgcgc caggctctga
     1921 tcgacctggg gctcgatgac ggccattgcc gccagctggg catccgcgtg cacaaggtga
     1981 acgtggtctg gccgctggag gccggcatca cgcgcgactt tgccgagggg ctgcaggaga
     2041 tcctggtggt cgaggaaaag cgccaggtca tcgaatacca gatcaaggag cagctctaca
     2101 actggcgcgc cgacgtgcgc ccggacgtgc tgggcaagtt cgacgaggtc gagggcgacc
     2161 attcgggcgg cgagtggggc atgcccaacc ccagccagaa ctggctgctg cgcccccagg
     2221 ccgacctgac gccggccatc atcgcgcgcg ccatcgccaa gcggctgaag aagctgggcg
     2281 tccccgcgga catcgcggcg cgcatggacg agcgcctgtc ggtgatagag gcgcgtgagc
     2341 gcggcatggc cgagcagaag ctggacaccg gcgagcgcat gccctggttc tgcagcggct
     2401 gcccgcacaa caccagcacc cgcgtgcccg agggcagccg tgccgtggcc ggcattggct
     2461 gccactacat ggtcaactgg atgcccgacc gccacaccag taccttcacg cagatgggcg
     2521 gcgagggcgt gacctgggtg ggccaggcgc cgttttccaa gaccccccat gtcttcgcca
     2581 atctgggcga cggcacctac ttccacagcg gcctgctggc gatccgccaa gcaatagcgt
     2641 ctggcgccaa cattacctac aagatcctct acaacgacgc cgtggccatg accggcggcc
     2701 agcaggtggg cgagcggccc gagggccact cggtcgcgca gatcgcccac agcctgcgct
     2761 ccgagggcgt ggcgcgcctg gtggtggtga ccgacgagcc cgagaagtac cacggccgca
     2821 cgcacagcct ggccgccaac cccgcgcgcg ccaagcacca ggagctgctc aacgacctgc
     2881 cgccgggtgt cgaggtattc caccgcgatg agctcgaccg catccagcgc gagctgcgcg
     2941 acatcgccgg ctgcacggcg ctgatctacg accagacctg cgccaccgaa aagcgccggc
     3001 gcaggaagcg cggcacgctt gcagcgccga ccaagacggt catcatcaac gagctggtct
     3061 gcgagggctg tggcgactgc ggggtggcgt ccaactgcct gtcggtggag ccggtcgaaa
     3121 ccgaattcgg ccgcaagcgc cgcatcaacc agagcacctg caacaaggac tattcctgcg
//
LOCUS       DAOMTQ010000241         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_8688_length_3173_cov_23.897049, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000241 DAOMTQ010000000
VERSION     DAOMTQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8688_length_3173_cov_23.897049"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..588
                     /gene="folK"
                     /locus_tag="PK518_15200"
     CDS             109..588
                     /gene="folK"
                     /locus_tag="PK518_15200"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRM50307.1"
                     /translation="MSAWIGLGANLGDARAALRSAVQAMAALPGTRLLRVSSLYRSAP
                     VDAGGPDYLNAVAELDTTLVPLELLHALQRIEQAAGRERPYRNAPRTLDLDLLLYGTL
                     RLDSAELTVPHPRMGERAFVLLPLAELAPPLVDAQQLAAVAGQRMECVATAQQWLQN"
     gene            complement(673..1644)
                     /locus_tag="PK518_15205"
     CDS             complement(673..1644)
                     /locus_tag="PK518_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM50308.1"
                     /translation="MNRRQLLLSAAAVAAASVTSLGHAQDGTIRLVVPYAPGGPIDVT
                     ARALAERVRDSLGTVIIDNKAGAGGNIGADLVAKSAPDGLTIGIAATATHAVNPWLYS
                     RMPYDAARDFAAITQMVRVPNVLVVNAARAEQLKIHSLQDLIAYARANPGKLNYGSGG
                     NGSAGHLAGEMFKQKAGIYALHIPYRGASPAQLALLAGEVDFNIDNLAAAAPNIKAGK
                     LKALAVTSLAASPMLPGVPPLSSSFKGFAIDTWWGLVAPAGTPGPVIARLNKAFTDAL
                     RAPETKTRFEALMAEPVPTTPEQFDAFMAAERAKYQQVVKASGAKVD"
     gene            complement(1718..2779)
                     /locus_tag="PK518_15210"
     CDS             complement(1718..2779)
                     /locus_tag="PK518_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HRM50309.1"
                     /translation="MTSPQTAPAAFQISVQPSGRTFQAQGDEAILAAAIRSGVGLPYG
                     CKDGACGSCKCKKLSGSVVHGEHQHKALSVEEEDAGYVLTCCARALTDVVLESRQVTD
                     ESAYPIKKLPVRVTTLTRASHDVMQLRLQLPANDQFRYHAGQYIEFILRDGSRRAYSM
                     ANAPHTQEATPGLELHIRHMPGGKFTDHVFGAMKEKEILRVEGPFGSFFLREDSKKPM
                     ILLASGTGFAPIKALIERMQHQKITRPAALYWGGRRPRDLYMDGWIRERLADLPQLYY
                     VPVVSDALPEDEWTGRTGLVHQAVLDDFADLSGFQVYACGAPIVVDSARTAYGEQRGL
                     PAEEFFADSFTSEADKHGG"
     gene            2845..>3173
                     /locus_tag="PK518_15215"
     CDS             2845..>3173
                     /locus_tag="PK518_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRM50310.1"
                     /translation="MPSNQNPLGALPARFRRQRLLIIGCGDIGLRVARGLRSRLQVLA
                     LTQSPERVPALRAAGITPLVGDLDAAASLARLAGLATRVLHLAPPPALGEIDARSLAL
                     ARALRRRA"
BASE COUNT          450 a         1057 c         1137 g          529 t
ORIGIN      
        1 ggccggcgct gatggcgctg atggcgaggc cgcagcgtcc aagaagcgcc gccggcgccg
       61 ccgcaaaccc gccgggggcg gcgaggcagg cacgggctca gacctggcgt gagcgcctgg
      121 ataggcctgg gtgccaacct gggcgacgcg cgcgccgccc tgcgcagcgc cgtgcaggcc
      181 atggcagcct tgccgggcac gcggctgctg cgcgtgtcat cgctctaccg cagcgcgccc
      241 gtcgatgccg gcgggcccga ttacctcaat gccgtggccg agctggacac cacgctggtc
      301 cccctggagc tgctgcacgc cctgcagcgg attgaacagg ccgcgggccg cgagcgcccc
      361 taccgcaacg cgccgcgcac gctggatctg gacctgctgc tgtatggcac gctgcggctg
      421 gacagcgccg agctcaccgt gccccacccg cgcatgggcg aacgcgcctt cgtgctgctg
      481 cccctggcgg aactggcacc gccgctggtg gatgcgcagc aactcgccgc cgtggccggc
      541 cagcgcatgg agtgcgtggc gacggcgcag cagtggctgc aaaattgata gctgttcgcg
      601 cttgtcattt ggcgtttatg aatggaaaac atttgataaa cgccatccat caagcgtgag
      661 tagctattgt ttttagtcca ccttggcgcc cgatgccttc accacctgct ggtatttggc
      721 gcgctcggcg gccatgaagg cgtcgaactg ctcgggcgtg gtgggcacgg gctcggccat
      781 cagggcctca aaacgggtct tggtctcggg ggccctgagg gcatcggtga aggccttgtt
      841 cagccgggcg atcaccggcc cgggcgtgcc ggcgggcgcc accaggcccc accaggtgtc
      901 gatggcaaaa cccttgaagc tgctcgacag cggcggcacg ccgggcagca tggggctggc
      961 ggccagtgag gtcacggcca gtgccttgag cttgccggcc ttgatgttgg gcgcggcggc
     1021 agccaggttg tcgatgttga agtccacctc gccggccagc agcgccagct gcgccgggct
     1081 ggcgccgcgg taggggatgt gcagggcgta gatgccggcc ttctgcttga acatctcgcc
     1141 cgccagatgg ccggcgctgc cgttgccgcc gctgccgtag ttgagcttgc cggggttggc
     1201 gcgggcgtag gcaatcaaat cctgcaggct gtggattttg agctgctcgg cgcgcgcggc
     1261 gttgaccacc agcacattgg gcacgcgcac catctgcgtg atggcggcaa agtccctggc
     1321 cgcgtcgtag ggcatgcggc tgtacagcca ggggttgacg gcatgcgtgg ccgtggcggc
     1381 aatgcctatg gtcaggccat cgggcgcgga cttggccacc aggtcggcgc caatgttgcc
     1441 gccggcgccg gccttgttgt cgatgatcac cgtgcccagg ctgtcgcgca cgcgctcggc
     1501 cagggcgcgc gcggtgacgt cgatggggcc accaggcgca tagggcacga ccaggcggat
     1561 ggtgccgtcc tgggcatggc ccagggaggt gacggaagct gcggcaacgg ccgctgccga
     1621 aagaagaagt tgtctgcggt tcatggatgg gcgtgagaga aagaaaagcg atatcggctg
     1681 cccgctggca gcctgcggtg tgcgcggttg ggcgatttca gccgccgtgc ttgtcggctt
     1741 ccgaggtgaa ggagtcggcg aagaactcct ccgccggcag gccgcgctgc tcgccatagg
     1801 cggtgcgcgc cgaatcgacc acgatgggcg cgccgcaggc atagacctga aagcccgaga
     1861 ggtcggcaaa gtcgtccagc acggcctggt gcaccaggcc tgtgcggccc gtccattcat
     1921 cttcgggcag ggcgtcggac accacgggca cgtagtacag ctggggcagg tcggccaggc
     1981 gctcgcgtat ccagccgtcc atgtacaggt cacgcgggcg gcgcccgccc cagtagagcg
     2041 cggccgggcg cgtgatcttc tggtgctgca tgcgctcgat cagcgccttg atgggcgcaa
     2101 agcccgtgcc cgaggccagc aggatcatcg gcttcttgga gtcctcgcgc aggaagaagc
     2161 tgccaaacgg cccctccacg cgcaggattt ccttctcttt catggcgccg aacacatggt
     2221 cggtgaactt gccgccgggc atgtggcgta tgtgcagctc caggccgggc gtggcctcct
     2281 gggtgtgcgg cgcgttggcc atggagtagg cgcggcgcga gccatcgcgc aatatgaatt
     2341 cgatgtactg gcccgcgtgg tagcgaaact ggtcgttggc cggcagctgc aggcgcagct
     2401 gcatcacgtc gtgcgaggcg cgcgtgagcg tggtcacgcg caccggcagc ttcttgatgg
     2461 ggtaggcgct ctcgtcggtc acctggcgcg actccagcac cacgtcggtc agcgcgcgcg
     2521 cgcagcaggt cagcacatag ccggcgtctt cttcctccac gctcagcgcc ttgtgctggt
     2581 gctcgccatg caccaccgag ccgctcagct tcttgcactt gcaggagccg caggcgccgt
     2641 ccttgcagcc atagggcagg cccacgccgc tgcggatggc ggccgccagt atggcttcgt
     2701 cgccctgcgc ctggaatgtg cggccgctgg gctgcacgct gatctgaaag gccgctggcg
     2761 cggtctgtgg actcgtcatg tatcggtatc cttggatgtt ttcatgccgg ggccgaggcc
     2821 ccgcctcgct cgacggagcc gattttgccc tcaaaccaaa accctctcgg cgccctgccg
     2881 gcgcgctttc ggcgccagcg cctgctcatc atcggctgcg gcgacatagg cctgcgcgtg
     2941 gcgcgcggcc tgcgctcgcg cctgcaggtg ctggcgctga cccagtcgcc cgagcgcgta
     3001 cccgcactgc gcgccgccgg catcacgcca ctcgtgggtg acctggacgc ggccgcgtcg
     3061 ctggcgcgcc tggccggcct ggccacgcgc gtgctgcacc tggcgccccc cccggcgctg
     3121 ggcgagatcg atgcgcgcag cctggcgctg gcgcgtgcgc tgcgccggcg tgc
//
LOCUS       DAOMTQ010000242         3147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_8806_length_3147_cov_25.790427, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000242 DAOMTQ010000000
VERSION     DAOMTQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8806_length_3147_cov_25.790427"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1674)
                     /gene="rlmD"
                     /locus_tag="PK518_15220"
     CDS             complement(220..1674)
                     /gene="rlmD"
                     /locus_tag="PK518_15220"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM50311.1"
                     /translation="MSEPTDNFPAPQEPSCDLPPGWLEVTSMDMDAQGVARRPDGKVV
                     FIDGALPTELVSANTHRKKNNWEQASLTAIHRESSQRVRPGCPHFGLHAGACGGCKMQ
                     HLDASAQVAMKQRVLEDNLWHLAKVKAETILRPIQGPTWGYRYRARLSVRYVAKKGVV
                     LVGFHERKSRYIADMQTCKILPPHVDAMLMPLRALIGSMAARDTCPQIELACGDSVTA
                     LVLRHLEPLCDADKDQLRAFAAQHGVQWWLQPKGPDTVHLLDEGGEQLAYGLPDFGIN
                     MPFRPTDFTQVNPHINRVLVTRALRLLDAQKHERVIDWFCGLGNFTLPIATQAREVLG
                     VEGSEALVARSRENYKLNQAQRPQGQALETTDFVARNLFEMTPETLIADGVADKWLVD
                     PPREGAFALSKALADIHQARLGAEGAPALPAGHAGWQPPRRIVYVSCNPATLARDAGL
                     LVHQAGYRCTAAGVVNMFPHTAHVESMAVFERAV"
     gene            complement(1728..2021)
                     /locus_tag="PK518_15225"
     CDS             complement(1728..2021)
                     /locus_tag="PK518_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50312.1"
                     /translation="MTAIRLAATAAAALVAGLFAQGSWAACYIVYGADKQIIYRSQLP
                     PVDMSRPLHETVPRIAPGGTLVFSLDSNGCELEIHNLPRAASKPARRDAGERR"
     gene            complement(2057..2581)
                     /locus_tag="PK518_15230"
     CDS             complement(2057..2581)
                     /locus_tag="PK518_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRM50313.1"
                     /translation="MLPDAAVVQAANALGQSLIARHGLQAQVRAERLHVTLMSLGWAH
                     ALSAEQLAWARGAAARVSVAPFTLRLDRALSFDRPARAKRPCVLCGPGDAHAGFHALH
                     VALHAALWPARPVPAVMPHMTLCYSHQAVPAQAVAPLEWTVRRFALLHNLRGSSGPYE
                     VLGEWPLDAAGTGQ"
     gene            complement(2639..2896)
                     /locus_tag="PK518_15235"
                     /pseudo
     CDS             complement(2639..2896)
                     /locus_tag="PK518_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
BASE COUNT          546 a         1048 c         1020 g          533 t
ORIGIN      
        1 actaggcaca tacctcagac tgggccgcga accctcaccc ccaccctctc ccagagggag
       61 agggagtaaa gccggcgcaa ccggtcgccc tcgccccttt ggggagaggg aggggtgagg
      121 ggcatctgcg acatgtacat agtcaggaaa aaatgcctga gcagcggcat gtgctgagca
      181 tgccaccctc aggcaaacaa acgcccaccg tcaaacgcgt caaacggccc gctcgaacac
      241 cgccatgctc tccacatggg ccgtgtgcgg gaacatgttg accacgcccg ccgccgtgca
      301 gcggtagccc gcctggtgca ccagcaggcc ggcgtcgcgc gccagcgtcg ccgggttgca
      361 gctcacgtag acgatgcgcc ggggtggctg ccagcccgca tgcccggcgg gcagcgcggg
      421 cgcgccctcg gcgcccaggc gcgcctggtg gatgtcggcc agggccttgg acagggcaaa
      481 ggcaccctcg cgcggcggat cgaccagcca tttgtcggcc acgccgtcgg cgatcagcgt
      541 ctccggcgtc atctcgaaca ggttgcgcgc tacaaaatca gtagtttcca gcgcttgccc
      601 ctgcggcctt tgcgcctgat tcagcttgta attctcacgc gatcgtgcga ccagtgcctc
      661 actgccctcc acgcccagca cctcgcgcgc ctgcgtggca atgggcagcg tgaaattgcc
      721 caggccgcag aaccagtcga tcacgcgctc atgcttttgc gcgtccagca ggcgcagcgc
      781 gcgcgtcacc agcacgcggt tgatgtgcgg attgacctga gtgaagtccg tgggcctgaa
      841 cggcatgttg atgccgaagt ccggcaggcc ataggccagt tgctcgcccc cctcgtccaa
      901 caggtgcacg gtgtccggcc ccttgggctg cagccaccat tgcacgccgt gctgggcggc
      961 gaaggcgcgc agctgatcct tgtcggcgtc gcacaggggc tccaggtggc gcagcaccag
     1021 ggccgtgaca ctgtcgccgc aggccagctc gatctgcggg caggtgtcgc gcgccgccat
     1081 gctgccgatc agggcgcgca gcggcatcag catggcatcc acatgcggcg gcaaaatctt
     1141 gcaggtctgc atgtcggcga tatagcggct cttgcgctcg tgaaagccga ccagcaccac
     1201 gcccttcttg gccacgtagc gcaccgacag gcgcgcgcgg tagcggtagc cccaggtcgg
     1261 gccctggatg gggcgcaata tcgtctcggc cttgaccttg gccaggtgcc agaggttgtc
     1321 ctccagcacg cgctgcttca tggccacctg ggcactggca tcgaggtgct gcatcttgca
     1381 gccgccgcag gcgcccgcgt gcaggccgaa atgcgggcag ccggggcgca cgcgctggct
     1441 cgactcgcgg tggatggcgg tcaggctggc ctgctcccag ttgttcttct tgcggtgggt
     1501 gttggcgctg accagctcgg tgggcagggc gccgtcgatg aacaccacct tgccgtccgg
     1561 acggcgcgcc acgccctggg cgtccatgtc catggaggtg acttcgagcc agccgggcgg
     1621 caggtcgcag gagggttctt ggggtgcggg aaagttgtct gtgggctcac tcatgcccgc
     1681 aattgtctca ggctcgcccc ttccctacgg gcgtggggcc gtgattttca gcggcgctca
     1741 ccagcgtcgc gccgtgctgg cttgctggcc gcgcggggca ggttgtggat ctccagctcg
     1801 cagccgttgg agtccagcga aaacaccagc gtgcccccgg gcgcaatgcg cggcacggtc
     1861 tcgtgcagcg ggcgcgacat gtccaccggc ggcagctgcg agcggtagat gatctgcttg
     1921 tctgccccat agacgatgta acaggcggcc cagctgccct gggcaaacag gccggccacc
     1981 agggctgccg cggcggtcgc ggccaggcga atggctgtca tggttggcta ccttcccgta
     2041 gtctgttcag gggcgattat tgcccagtgc ctgcggcatc cagcggccac tcgcccagca
     2101 cctcataggg gccgcttgag ccgcgcaggt tgtgcagcaa cgcaaagcgg cgcaccgtcc
     2161 attccagtgg cgccacggct tgcgcgggta cggcctggtg gctgtagcac aacgtcatgt
     2221 gcggcatgac ggctggtacc ggcctggcgg gccacagcgc cgcgtgcaag gctacatgca
     2281 gcgcatgaaa cccggcatgc gcgtcacccg gcccgcacag cacgcagggg cgcttggcgc
     2341 gcgccggacg gtcaaagctc aaggcgcgat ccaggcgcaa cgtgaacggc gccaccgaga
     2401 cacgcgccgc cgcgccgcgc gcccatgcca gttgctcggc agacagcgca tgcgcccagc
     2461 ccagcgacat cagcgttaca tgcaaacgct cggcgcgcac ctgggcctgg aggccgtggc
     2521 gcgcaatcag cgactggccc agggcgttgg ccgcctgcac caccgcggca tcgggcagca
     2581 cggcgaaaaa caaatggtgc cggggctgcg gtccggcatt gaattgtgtt gagtcaccca
     2641 gcaatgcact cagcgcctgg ttgtgcgtga acgccgccac ccgccgctca ttggccagca
     2701 tggccagaaa accctcgacc tcctcccacc ccatgtcgcg cggatgccgc aaaccatgcc
     2761 acttcacaac cttgcgcacc cattgcacat aggcgtcctc ggtacgcagg ctatcgtgtt
     2821 tgtaacgaat tcgctcacgg acttgatcca gcaaccgtgt agtgcgtaac accggggaag
     2881 gagtagcggg tttcatggct gaattgagtg ctgtattttt gtgcagtatt ccgactgcca
     2941 agtcaggctg caagcccctt gcagcctttt ttgcgcggca atttagaccg cagatgcaag
     3001 ttagatctct caagctaatg acatggttca caccgtcata aaacccattt cggagttcgc
     3061 agctgcacta gcagaaattc cgcttagccc tggaaagcac gttttacaag gtggctagac
     3121 atgaagactg aatgcgacct cgccaag
//
LOCUS       DAOMTQ010000243         3146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_8811_length_3146_cov_24.872857, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000243 DAOMTQ010000000
VERSION     DAOMTQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8811_length_3146_cov_24.872857"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK518_15240"
     CDS             <1..199
                     /locus_tag="PK518_15240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRM50314.1"
                     /translation="DGSQPLRQAAGGASQVWVLHGPEGGLTAQEEDAALAAGFVPASL
                     GTRVLRAETASVAALAVLGLE"
     gene            complement(226..1803)
                     /locus_tag="PK518_15245"
     CDS             complement(226..1803)
                     /locus_tag="PK518_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRM50315.1"
                     /translation="MRFTSRLILCAVVPAGLFVLALAISLWGLTRTQSDFSRYMGTEQ
                     AVAAGLSELYAQGLQSGQALRNIVLDPANQVAYDNLKASHQAFDEAYEATLAVVRGTP
                     VEAALLPLKQLRVTQVGVQGQVRDLARSDPAAAAQALNSLETPAWRALRSALLQQIKV
                     ARATADEVHRSTQARAERMQRIAAAVALFAALAAAALTVVMVRTLRLELGGEPAEARQ
                     ALGRVAEGDLSAAAGHGVARGLMAELVRMRASLRSLVTRVHGTAVEITHASGEIAQGN
                     ADLSARTESQASALEETAASMEQLSATVRQNADNARAANQLALNASNVAAQGGEVVSQ
                     VVDTMKGINDSSRQIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR
                     SLAQRSAEAAKQIKELITASVERVQQGSCLVDQAGATMGEVVAAIRRVTDIMGEISAA
                     SGEQSAGVAQVGEAITQMDQATQQNAALVEESAAAAAALKKQAQELLAAISQFRLADG
                     GMPEALPAGASPRLLAA"
     gene            complement(2122..>3146)
                     /locus_tag="PK518_15250"
     CDS             complement(2122..>3146)
                     /locus_tag="PK518_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM50316.1"
                     /translation="KRQGVPHPRIVGLIETCEESGSADLLPYIDALRTRLGEVALVIC
                     LDSGAGNYDQLWLTTSLRGMASGTLKVEILTEGIHSGDASGLVPSSFRIMRQVLNRLE
                     DSATGRLLPARFHCEVPADRLAQARATAAILGEEVYRRFPWAHHDCGGSQVFALPTTT
                     DPVQALIKRTWEPTLSVTGAEGFPALKDAGNVLRPYTAFKLSLRLPPLVDAATAVQEL
                     KTLLEDNAPYQARVTWEGASAASGWNAPPITPWFEQALNAASQAQFGAPCGYIGQGGT
                     IPLMNMLSQGFPKAQMMVCGVLGPKSNAHGPNEFLHVPYAKRLTAAVAQVIAAMPGCM
                     QAHAEV"
BASE COUNT          436 a         1083 c         1125 g          502 t
ORIGIN      
        1 tgacggcagc cagccactgc gccaggccgc cggcggcgcc agtcaggtct gggtgctgca
       61 cggccccgag ggcgggctga cggcgcagga ggaagacgcg gcgctggccg caggctttgt
      121 ccccgccagc ctgggcacgc gcgtgctgcg cgccgagacg gcctccgtcg ccgccctggc
      181 cgtgctgggg ctggaatgat ggctgcggtc cgcgccgccg ccgcttcagg cggcaagcaa
      241 ccggggcgac gcgccggccg gcagcgcctc gggcatgccg ccgtcggcca agcggaactg
      301 cgagatcgcg gcgagcagct cctgggcctg tttcttcagc gccgcggctg cggccgcgct
      361 ttcctcgacc agcgccgcgt tctgctgcgt cgcctgatcc atctgcgtga tcgcctcgcc
      421 gacctgggcc acgccggcgc tttgctcgcc gctggccgcg ctgatctcgc ccatgatgtc
      481 ggtcacgcgc cgtatcgccg ccaccacctc gcccatggtc gcgccggcct gatcgaccag
      541 gcatgagccc tgctgcaccc gctccacgct ggccgtgatc agctccttga tctgcttggc
      601 cgcctcggcg ctgcgctgcg cgaggctgcg cacttcgctg gccacgacgg caaagccgcg
      661 gccctgctcg ccggcgcgcg ctgcctccac ggccgcgttc agcgccagga tgttggtctg
      721 gaaggcaatg ccgtcgatca cgccgatgat gtccgcgatc tggcggctgc tgtcgttgat
      781 gcccttcatg gtgtccacga cctgggacac gacctcgccg ccctgggccg caacattgga
      841 ggcgttcagc gccagctggt tggccgcgcg cgcgttgtcg gcgttctggc gcacggtggc
      901 gctgagctgc tccatggagg ccgcggtttc ttccagcgca gaggcctggc tttcggtgcg
      961 cgccgacagg tcggcattgc cctgggcgat ttcgccggac gcatgggtga tttcgacggc
     1021 cgtgccgtgc acccgggtca ccaggctgcg caggctggca cgcatgcgca ccagctcggc
     1081 catcagtccg cgcgccacgc catgcccggc ggcggcgctg agatcgccct cggccacccg
     1141 gccgagcgcc tggcgcgcct cggcgggctc gccgcccagc tccaggcgca gcgtgcgcac
     1201 catgaccacg gtcagggcgg ccgccgccag ggccgcgaac aaggccacgg ccgccgcgat
     1261 gcgctgcatg cgctcggccc gggcctgggt gctgcggtgc acctcgtcgg ccgtggcgcg
     1321 tgcgaccttg atctgctgga gcagcgccga gcgcagcgcg cgccaggccg gcgtttccag
     1381 gctgttcagc gcctgggccg ccgccgcggg gtcgctgcgc gccagatcac gcacctggcc
     1441 ttgcacgccc acctgggtca cgcgcagctg cttgagcggc agcagcgcgg cttccaccgg
     1501 cgtgccgcgg acgaccgcca gcgtggcctc gtaggcctcg tcgaaggcct ggtgcgaggc
     1561 cttgaggttg tcgtaggcca cttgattggc cgggtcgagc acgatgttgc gcagcgcctg
     1621 gcccgattgc aggccctggg cgtagagctc ggacaggccg gccgccacgg cctgctcggt
     1681 gcccatgtag cggctgaaat cggattgggt gcgcgtcagc ccccacaggc tgatggccag
     1741 ggccagcacg aacaggccgg ccggcacgac ggcgcaaaga atcaaacggg aagtgaatcg
     1801 catcgggatg cctctcgcag ccaacgggtc atcgctggcc gttaataaat ataaattgtt
     1861 aacaatgtaa ccaaaagatg tggcctgcga gactaggata aacacgtggc cgccgaggac
     1921 tccggtgcgt cttgcttcag cgccgcgggc cttggaaagg cccgcaacgg cacaaggccg
     1981 tgccgcccgg gagattcggc ggggtgcgcg cgcggcccgg ccatggcggc gcaggcgctc
     2041 gccgccatgc cgggttcgca ttttgctatt aataatatag ctgtttgcgc tttaccagta
     2101 agtgtaaaac cccaatttca ttcaaacttc cgcgtgcgct tgcatgcagc cgggcatggc
     2161 ggcgatcacc tgcgccacgg ccgcggtgag cctcttggca tagggcacat gcaggaactc
     2221 attggggcca tgggcgttgc tcttggggcc gagcacgccg cagaccatca tctgcgcctt
     2281 cgggaaaccc tggctcagca tgttcatcag cgggatcgtg ccgccctggc cgatgtagcc
     2341 gcagggggca ccaaactgcg cctggctggc ggcgttcagg gcctgctcaa accagggggt
     2401 gatgggcggg gcgttccagc cgctggcggc actggcgccc tcccaggtga cgcgcgcctg
     2461 gtagggggcg ttgtcttcca gcagggtttt cagctcctgc acggcggtgg cggcgtccac
     2521 cagcggcggc aggcgcaggc tgagcttgaa ggcggtgtag gggcgcagca cgttgccggc
     2581 gtccttcagc gccggaaagc cctcggcgcc ggtgacgctc aaggtgggct cccaggtgcg
     2641 cttgatcaac gcctgcaccg ggtcggtggt ggtgggcagt gcaaatacct ggctgccacc
     2701 gcagtcgtgg tgcgcccagg ggaagcgccg gtagacctct tcgcccagga tggcggccgt
     2761 ggcgcgcgcc tgggccaggc ggtcggccgg cacctcgcag tgaaagcgcg cgggcagcag
     2821 gcggccggtg gccgagtcct ccaggcggtt cagcacctgg cgcatgatgc ggaacgacga
     2881 gggcaccagg ccggatgcgt cgccggaatg gatgccctcg gtcaggatct cgaccttgag
     2941 cgtgccgctg gccatgccgc gcaggctggt ggtgagccac agctggtcgt agttgccggc
     3001 gcccgagtcc aggcagatca ccagggccac ctcgcccagg cgggttctca aggcgtcgat
     3061 atagggcagc aggtcggcgg agccgctttc ctcgcaggtc tcgatcaggc cgacgatgcg
     3121 cgggtgcggc acgccctggc gcttca
//
LOCUS       DAOMTQ010000244         3103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_8988_length_3103_cov_27.329724, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000244 DAOMTQ010000000
VERSION     DAOMTQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8988_length_3103_cov_27.329724"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PK518_15255"
     CDS             complement(<1..156)
                     /locus_tag="PK518_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRM50317.1"
                     /translation="MPRRALLLLTALVLILHWLVLVGLPLGGGAGAAPQPTAFHTRML
                     SAPPAAPP"
     gene            234..1217
                     /locus_tag="PK518_15260"
     CDS             234..1217
                     /locus_tag="PK518_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRM50318.1"
                     /translation="MKLATYKDGSRDGQLVVVARDLGAAHYATGIASRLQQVLDDWNF
                     LAPQLQDLYEQLNAGRARHAFAFDARQCMAPLPRAYQWADGSAYLNHVELVRRARGAE
                     VPASFYTDPLMYQGGSDDFLGPCDDVVVPSEAMGIDFEAEIAVITGDVKMGASPEQAL
                     DGVRLLMLANDVSLRNLIPAELAKGFGFFQSKPATAFSPVAVTLDELGDAWQGGRVHL
                     TLQSTWNGRRVGMCDAGAEMSFHFGQLIAHIAKTRNVRAGSIVGSGTVSNKDWSKGYS
                     CIAEKRCIETIEDGRPSTEFMKYGDTIRIEMKGRDGQSLFGAIDQQIVSHG"
     gene            1486..1953
                     /locus_tag="PK518_15265"
                     /pseudo
     CDS             1486..1953
                     /locus_tag="PK518_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349163.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
     gene            2140..2340
                     /locus_tag="PK518_15270"
                     /pseudo
     CDS             2140..2340
                     /locus_tag="PK518_15270"
                     /inference="COORDINATES: protein motif:HMM:NF016283.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            2427..2918
                     /locus_tag="PK518_15275"
                     /pseudo
     CDS             2427..2918
                     /locus_tag="PK518_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044399455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          605 a          990 c          976 g          532 t
ORIGIN      
        1 gggtggcgcg gcgggcggcg ctgacagcat gcgcgtgtga aacgccgtcg gctgcggcgc
       61 cgcgccggcc ccgccgccca gcggcaggcc gaccagcacc agccagtgca ggatcagcac
      121 cagggcagtc agcagcagca gggcgcggcg tggcatggct agaattttct gctttgtgca
      181 atcaacccgc tgcgccacca tgggcgcggg cggtcagacg gagggcctgg cccatgaaac
      241 tggcgaccta caaggacggt tcgcgcgatg gtcagctggt tgtggtcgcg cgcgacctgg
      301 gcgcggccca ttatgcgacc ggcattgcca gccgcctgca gcaggtgctg gacgactgga
      361 acttcctcgc cccccagctg caggacttgt acgagcagct caacgccggc cgcgcgcgcc
      421 acgcgtttgc gttcgacgcg cgccagtgca tggcgccgct gccgcgcgcc taccagtggg
      481 ccgatggttc ggcctacctc aaccatgtcg agctggtgcg ccgcgcgcgt ggcgccgagg
      541 tgccggcgag cttttacacc gacccgctga tgtaccaggg cggcagcgac gattttctgg
      601 gcccctgcga cgatgtggtc gtgccgagtg aggccatggg catcgacttc gaggccgaga
      661 tcgccgtcat caccggcgac gtgaagatgg gcgcgagccc ggagcaggcg ctggacggcg
      721 tgcgcctgct gatgctggcc aacgacgtca gcctgcgcaa cctgataccg gcggagctag
      781 ccaagggctt cggcttcttc cagtccaagc cggccactgc cttcagcccg gtggccgtga
      841 cgctggacga actcggcgac gcctggcagg gcgggcgcgt gcacctgacg ctgcagtcca
      901 cctggaacgg ccgcagggtt ggcatgtgcg acgccggggc ggagatgagc tttcacttcg
      961 gccagctcat cgcccatatt gccaagacgc gcaacgtgcg cgccggctcc atcgtgggca
     1021 gcggcacggt gagcaacaag gactggtcca agggctatag ctgcattgcc gagaagcgct
     1081 gcatcgagac catcgaggac ggccggccga gtacggaatt catgaagtac ggcgacacca
     1141 tccgcatcga gatgaagggc cgggacgggc agagcctgtt cggcgccatc gaccagcaga
     1201 tcgtgtcgca cggttgattt agaggtgaat attcgcccag gccttgaagt acaagcgcaa
     1261 gaagctatgg ttttatgagt aactggcggc cgcctcgacc tgcaccgcgc agtcgtagaa
     1321 cgtaggcccg gcgcccaggt cggtcagggc ctggctggtg agctggttga tgttgcaact
     1381 gtccagccca tacttgtgtc ttcagctgcc acgcataatc gttaacgaca tgtttcgagg
     1441 aatgcagtgc ctcttgttcc ccatacgtcc taccagtccc aatacatcgc ctggctgctt
     1501 tcaaggcaga tcgggcgtga ctccccccga cctcctggcc tccaccctgg ttgatgcgcg
     1561 ggtcgatctg aatccgcacc aggtcgaggc agcactgttc gcgttccgaa gcccgctttc
     1621 gcaaggcgtg atcctggcgg acaaggtggg cctgggcaag accatcgagg ctggcctggc
     1681 gattgtccag cgctggacag agcgcaagcg ccgcatcctc atcatcaccc cggccaactt
     1741 gcgcaagcag tggcaccagg agctgcaaga caagttcagc ctgcaagcgc tgattctgga
     1801 agccaaaagt tacaacgagc agcgcaaggc gggtttgccc aacccctttg accaaaccca
     1861 ggaccaagcc agccagatcg tcatctgctc ctaccaacca aggccgatga cctgtgccgc
     1921 gtgccctgcg atcaggtggt ggtgatcaat gagctggagg ggaggctctg ttcgacatcg
     1981 gccctcgaag aggtattcaa actccaatgg gagttggcat gaagaaccgg atcatcaacg
     2041 tcaaaggagc ggaggtctcc attgccaccc ggcatgatca ggaccacatc taccgatatg
     2101 gtcaagaggt tcggagacga aagcatcctg tacaactgga tgcgcaaccg caacaccatt
     2161 gagtttctgg gtatttggga gcaaatctac aacccgcatt tcaaacctct cgaattcgag
     2221 aggtttagaa ggcaagctgg tctgaacagc ttctctctgt cacccaaaaa atggattgag
     2281 gcaacaggtg ccatcggtct gtacgccaaa gcagggcgcg gaggcggcac ctatgcccac
     2341 aaagacattg ccttcgagtt tggctcctgg ctcagcccgg agttcaagct ctacctcagc
     2401 tggccgcgct gcaagcgcgt caagcctcca tcgaaaccat cgaatcgcaa gcgcccgggt
     2461 tctgccccca ttgcggcgcc gcccacatcg tcaagaacgg ctttgcccgc ggcctgcagc
     2521 gctttttgtg ccgtggctgc acatgcacct tcaacgcgct gacgggcgcg ccgctgagtg
     2581 ggctgcatca gcggggcaaa tggctggcgc acacccaggc gctcagccaa ggcctgagca
     2641 ttccccgggt ggccgagcgg ctggaggtgg cctggagcac ggcgttccgc tggcgtcacc
     2701 gcttcctgca gctgcccagt cagcgccagg ccagcgcatt gcgcggcatt gccgaggccg
     2761 atgagtccta cttcctcgaa tcgagcaaag gccagcgtgc attgctgcgc caggcgcgcc
     2821 ggcgcggcgg caaggccagc cagcgcggcg tctccaagga gcaggtatcc gtgctcatgg
     2881 tgcgcgaccg tgcgggcgcc acggccaaca tcctcgtcgc gcgtaaacgg tgttaactgt
     2941 ctggcggcaa ctcgccagcg aggcagtcac accgacctca atttaaacca accagcctcc
     3001 tcgaaatccg gggctgttca tgtcgaggca aacgataaag cctacacatc agaacaccga
     3061 ccagccggtg aactctgaca actcttgcag cgcgcgcgtg ccg
//
LOCUS       DAOMTQ010000245         3072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9127_length_3072_cov_27.052038, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000245 DAOMTQ010000000
VERSION     DAOMTQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9127_length_3072_cov_27.052038"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PK518_15280"
     CDS             complement(<1..326)
                     /locus_tag="PK518_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRM50319.1"
                     /translation="MTERGAGRSYMARLNRIMALYVVGLLGFVGLMAWAEQQGLSRQW
                     IGPIFLFLTVMAYAGIGVYARTTDADEYYVAGRRIPPFYNGMAAAADWMSAASFISLS
                     GALYLQG"
     gene            complement(319..648)
                     /locus_tag="PK518_15285"
     CDS             complement(319..648)
                     /locus_tag="PK518_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRM50320.1"
                     /translation="MPQRRTFAESQIEGDYEGAPFAPDLHDARHLWLKAVLLLVWLLV
                     SFVACYFARDLPVTVGGWQLSYWLAAQGAVLVFIVIVGVYCVAMDHFERQDMAAQGQP
                     GAAQAHD"
     gene            complement(656..3046)
                     /gene="ppsA"
                     /locus_tag="PK518_15290"
     CDS             complement(656..3046)
                     /gene="ppsA"
                     /locus_tag="PK518_15290"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRM50321.1"
                     /translation="MSARFEATALVVPFENLRMSDVEAVGGKNASLGEMISQLPQGVR
                     VPTGFATTAHAFREFLKHEGLAERISARLATLDVDDVRALAAAGAEIRGWLENQPFPA
                     DLEKAVRAAFAELSAGNAQASFAVRSSATAEDLPDASFAGQQETFLNVVGIEDVLHKM
                     KEVFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDLGAAGVMFTIDTESGFDQV
                     VFITSSYGLGETVVQGAVNPDEFYVHKPMLQAGKQAVIRRNLGSKLIQMVFATPEEKA
                     ASGKLVKTTDVAPELRNRYSLSDAEVQQLAHYALVIEQHYGRPMDIEWGRDGTDGQLY
                     ILQARPETVKSQAKGQAEQRYKLKGHGTVLAEGRAIGQKIGTGRVRLVHDIAEMDKVQ
                     AGDVLVTDMTDPNWEPVMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGHATELLK
                     DGTLVTVSCAEGDTGHIYDGLLETEVTEVQRGEMPQISTKIMMNVGNPQLAFDFAQLP
                     NEGVGLARLEFIINNNIGVHPKAILDYPAVDADLKKAVESVARGHASPRAFYVDKVAE
                     GVATIAAAFYPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGAARYISKDFG
                     EAFAMECEALKRVRNDMGLANVQIMVPFVRTLGQAERVTGLLAAHGLKRGENELKLIM
                     MCEVPSNAILAERFLEYFDGFSIGSNDLTQLTLGLDRDSGLELLAQDFDERDPAVEAL
                     IHKVILACRAQGKYIGICGQGPSDHPDFAQWLAREGISSISLNPDSVIDTWHKLAG"
BASE COUNT          507 a         1044 c          987 g          534 t
ORIGIN      
        1 ccctgcaggt acagcgcccc cgacaggctg atgaacgagg ccgcgctcat ccagtcagcg
       61 gccgcagcca tgccgttgta gaacggcgga atgcgccgcc cggcgacgta gtattcgtcg
      121 gcgtccgtgg tgcgcgcgta gacacctatg ccggcgtagg ccatcaccgt caggaacagg
      181 aagatggggc ctatccactg gcgcgacagg ccctgctgct cggcccaggc catcaggccc
      241 acgaagccca gcaggccgac gacatacagc gccatgatgc ggttcaggcg cgccatgtag
      301 gagcgccctg ccccgcgttc agtcatgggc ctgggcagcg ccgggctggc cttgcgctgc
      361 catgtcctgc cgctcgaaat gatccatcgc cacgcagtac acgccaacga tgacgatgaa
      421 caccagcacc gccccttgcg ccgccagcca gtagctcagc tgccagccgc ccaccgtgac
      481 cggcaggtcg cgcgcgaagt agcaggcgac aaaggacacc agcagccaca ccagcagcag
      541 cacggccttg agccacaggt ggcgcgcgtc atgcagatcg ggggcgaagg gcgcgccctc
      601 gtagtcgccc tcgatctggc tttcggcgaa cgtcctgcgc tgcggcatgg ctcgttcagc
      661 cggcgagctt gtgccaggtg tcgatcacgc tgtcggggtt cagcgagatc gaggaaatgc
      721 cctcgcgcgc cagccactgg gcaaagtccg ggtgatcgct gggcccctgg ccgcagatgc
      781 cgatgtactt gccctgcgcg cggcaggcca ggatcacctt gtggatcagc gcctcgacgg
      841 ccgggtcgcg ctcgtcgaaa tcctgcgcca gcagctccag gcccgagtcg cgatcgagcc
      901 ccagcgtgag ctgggtcagg tcgttggagc caatggagaa gccgtcgaag tactcaagga
      961 agcgctcggc caggatggcg ttgctcggca cctcgcacat catgatcagc ttgagctcgt
     1021 tctcgccgcg cttgaggcca tgcgcggcca gcagccccgt cacgcgctcg gcctggccca
     1081 gcgtgcgcac gaagggcacc atgatctgca cgttggccag gcccatgtcg ttgcgcacgc
     1141 gcttgagcgc ttcgcattcc atcgcaaagg cctcgccaaa gtccttgctg atgtagcgcg
     1201 ccgcgccgcg aaagcccagc atggggtttt cttcctcggg ctcgtagcgg ctgccgccga
     1261 tcagcttgcg gtactcgttg ctcttgaagt ccgacaggcg cacgatgacc ggcttggggt
     1321 aaaaggccgc cgcaatcgtc gccacgccct cggccacctt gtccacgtag aaggcacgcg
     1381 ggctggcgtg gccgcgcgcc acgctctcca cggccttctt caggtcggca tcgacggcgg
     1441 ggtagtccag gatggccttg gggtgcacgc cgatgttgtt gttgatgatg aactccagcc
     1501 gcgccaggcc cacaccctca ttcggcagct gggcgaagtc aaaggccagc tgcggattgc
     1561 cgacattcat catgatcttg gtgctgatct gcggcatctc gccgcgctgc acctcggtca
     1621 cctcggtctc cagcaggcca tcgtagatgt ggccggtgtc gccctcggcg cagctcaccg
     1681 tcaccagcgt accgtccttc agcagctcgg tggcgtgacc gcagccgacc acggcgggga
     1741 tgccgagttc gcgcgcgatg atggcggcgt ggcaggtgcg cccgccgcgg ttggtgacga
     1801 tggccgaggc cttcttcatc accggctccc agttcgggtc ggtcatgtcg gtgaccagca
     1861 cgtcgcccgc ctgcaccttg tccatttcgg caatgtcatg aaccaggcgc acccggccgg
     1921 tgccgatctt ctggccaatc gcgcggccct cggccagcac ggtgccatgg cccttgagct
     1981 tgtagcgctg ctcggcctgg cccttggcct ggctcttcac ggtctcgggg cgcgcctgca
     2041 ggatgtagag ctggccgtcg gtgccgtccc tgccccactc gatatccatg gggcggccgt
     2101 aatgctgctc gatgaccaag gcgtaatgcg ccagctgctg cacctcggca tcgctcaggc
     2161 tgtagcggtt gcgcagctcg ggggcaacat cggtggtctt gaccagcttg cccgaggcgg
     2221 ccttttcttc gggagtagca aacaccatct ggatcagctt gctgcccaga ttgcggcgaa
     2281 tcaccgcctg cttgccggcc tggagcatgg gcttgtgcac atagaactcg tcggggttta
     2341 ccgccccctg caccacggtc tcgcccaggc cgtagctgct ggtgatgaac accacctggt
     2401 caaaaccact ctcggtatcg atggtgaaca tcacgccggc ggcgcccaga tccgagcgca
     2461 ccatgcgctg cacacctgcc gacagcgcca ccacgtcgtg cgcaaagccc ttgtgcacgc
     2521 ggtagctgat ggcgcggtcg ttgtagaggc tggcaaacac ctccttcatc ttgtgcagca
     2581 cgtcctcgat gccgacgacg ttcagaaaag tctcctgctg gccggcgaac gaggcatcgg
     2641 gcagatcctc ggccgtggcc gagctgcgca cggcaaagct ggcctgtgca ttgcccgccg
     2701 acagctcggc aaaggccgcg cgcacggcct tttccagatc cgccggaaag ggttggtttt
     2761 ccagccagcc gcgaatctcg gcaccggcgg cggccagcgc gcgcacatcg tccacgtcca
     2821 gcgtggccag gcgggcgctg atgcgctcgg ccaggccctc gtgctttaga aattcgcgga
     2881 aggcgtgcgc cgtggtggca aagccggtgg gcacgcgcac cccttggggc agctgcgaga
     2941 tcatctcgcc gaggctggcg ttcttgccgc cgacggcctc gacgtcgctc attctcaggt
     3001 tttcaaacgg tacgaccagg gcggtcgcct caaaacgtgc agacatggga aagctccaga
     3061 agttgaaacc gg
//
LOCUS       DAOMTQ010000246         3060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9187_length_3060_cov_23.388686, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000246 DAOMTQ010000000
VERSION     DAOMTQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_3060_cov_23.388686"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PK518_15295"
     CDS             complement(<1..1563)
                     /locus_tag="PK518_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM50322.1"
                     /translation="MPRLSQQLHPHREPVTPLNAATVLLLRDAPVAGGTVLEVLMTRR
                     SDKASFVPGAYVFPGGGIEAQDGDGASHALADRRPTQTDARLTDAMAAIRESFEELGV
                     LLARRADGSWADAADIAAIARKLPPETTFAAACAARGLRLAADKVYQLAHWTADRNLP
                     KRFAVPFLVARMPEGQEPVADESEQFEPVWVRPADALARHEAGQFFMIFPTIRTLQRL
                     AQFADVDAVLAAVAGEQPLWASSPRSGLLAGKEARFMEHEAPYGELALVTPNGRGEHV
                     LDWQSETPVPLLKNLQRLTAANPGVMTGPGTNSYLVGDPGTGYIAIDPGPADKAHLER
                     LWRAAGGDIRMIVCTHSHPDHSPGAAPLQTMVAAAGRPQPPVLGLPSAPTARAASQFT
                     PDRSLQNDELLTLEGQGPEGKITHTLQVVHTPGHAANHLCLLLVEDGLLFSGDHILNG
                     STTVIDPPDGNMADYLDSLDRLDALCAEHDVDFIAPAHGYVLGEARAAIARLKAHRLA
                     REAKVLAAMQAQP"
     gene            1623..2501
                     /gene="dapF"
                     /locus_tag="PK518_15300"
     CDS             1623..2501
                     /gene="dapF"
                     /locus_tag="PK518_15300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRM50323.1"
                     /translation="MHIRFTKMQGAGNDFVVLDETQEILGLTRAQYRFLADRHFGVGA
                     DQILSVRPSPAQGIDFEYLIHNADGGQVEQCGNGARCFARFVRDKGLTHKDEIRVQTM
                     AGVIAPRLEADGRVTVDMGRPVLEPERVPFDATGLQPVTQGFTQKWPLTFDGQAHEAT
                     VFIVPISMGNPHAVQLVDDVDTAPVAQTGSLIENHPRFAQRVNAGYLQIISRAEVRLR
                     VYERGAGETLACGTGACAAVVAGIGLGLLDGRVDVHTRGGLLSIAWAGGVQDPVLMTG
                     PAVTVFEGQIAIPDLP"
     gene            2498..>3060
                     /locus_tag="PK518_15305"
     CDS             2498..>3060
                     /locus_tag="PK518_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRM50324.1"
                     /translation="MNHSSVTPITEDDIADFLVNTPGFFERHAELLSAVTISSPHGQR
                     AVSLQERQAEMLREKIKGLEHRVMDMVRHGHENTAITHKIHQWSRELLAVQDVAELPQ
                     AVEQGIHRLFDVPQVALRLWGTAGMHADAPFTHGASEDVRSFASSLTMPFCGPNLGFE
                     ATAWLPQAGQAQSLALLPLRRGAIDSA"
BASE COUNT          456 a         1015 c         1096 g          493 t
ORIGIN      
        1 gggctgggcc tgcatggcgg ccagcacctt ggcctcgcgc gccaggcggt gggccttcag
       61 gcgggcgatg gcggcgcgcg cctcgcccag cacatagccg tgggccgggg cgatgaagtc
      121 cacgtcatgc tcggcgcaca gcgcatccag gcggtcgagc gaatccaggt agtcggccat
      181 gttgccgtcc ggcgggtcta tgaccgtggt gctgccgttg aggatgtggt cgcccgagaa
      241 cagcaggccg tcctcgacca gcagcaggca caggtggttg gccgcgtggc cgggcgtgtg
      301 caccacctgc agggtatggg taattttgcc ttctggccct tgtccttcaa gcgtgagcag
      361 ctcatcattt tgtagtgaac ggtcgggtgt gaactggctg gcggcgcgcg ccgtgggggc
      421 tgacggcagg cccagcacgg gcggctgcgg gcgcccggcg gccgccacca tggtttgcag
      481 cggcgcggcg cccggggaat ggtcggggtg cgaatgcgtg cagacgatca tgcgaatgtc
      541 gccgcccgcg gcgcgccaca gccgctccag atgcgccttg tcggccgggc cggggtcgat
      601 ggcgatgtag cccgtgccgg ggtcgcccac caggtagctg ttggtgcccg ggccggtcat
      661 cacgccgggg ttggcggcgg tcaggcgctg caggtttttc aggagcggca ccggggtttc
      721 gctctgccag tccagcacat gctcgccgcg gccgttgggt gtgaccaggg ccagctcgcc
      781 atagggcgcc tcatgctcca tgaagcgcgc ctccttgccc gccagcaggc ccgagcgcgg
      841 gctgctggcc cacagtggct gctcgcccgc gacggcggcc agcacggcat ccacgtcggc
      901 aaactgcgcc aggcgctgca gcgtgcgtat ggtcgggaag atcatgaaga actgcccggc
      961 ctcgtgccgc gccagggcgt ctgctgggcg cacccagacg ggctcgaact gctcggactc
     1021 gtcggccacc ggctcctgcc cttcgggcat gcgcgccacc agaaagggca cggcgaagcg
     1081 cttcggtaaa ttgcggtcgg cagtccaatg cgccagctgg tagaccttgt cggccgccag
     1141 gcgcaggccg cgcgcggcgc atgcggcggc aaacgtcgtc tccggcggca gcttgcgcgc
     1201 aatggcggca atgtcggcgg cgtcggccca gctgccatcg gcgcggcgcg ccagcagcac
     1261 gcccagctct tcaaagctct cgcggatggc ggccatggcg tcggtcaggc gcgcgtcggt
     1321 ctgcgtgggg cggcggtcgg ccagcgcgtg gctggcgccg tcgccgtcct gggcctcgat
     1381 gccgccgccg ggaaagacat aggcgccggg gacaaagctg gccttgtccg atcggcgcgt
     1441 catcaagacc tccagcaccg tgccgccggc gacgggggca tcgcgcagca gcagcacggt
     1501 ggcggcattc aggggcgtga cgggttcacg gtgggggtgg agttgttggc tgaggcgtgg
     1561 catcggcgca ttatgggcaa gccagggtga tcgcgccgat gcggcgcgga taatcgcgcc
     1621 ccatgcacat ccgctttacc aagatgcaag gcgcgggcaa cgactttgtg gtgctcgacg
     1681 agacgcagga aatcctgggt ctgacgcgcg cgcaataccg ctttctggcg gaccgccatt
     1741 tcggcgtggg cgccgaccag atcctcagcg tgcgcccctc gcccgcccag ggcatcgatt
     1801 ttgaatacct gatccacaac gccgacggcg gccaggtgga gcagtgcggc aacggtgcgc
     1861 gctgctttgc gcgctttgtg cgcgacaagg ggctgaccca caaggacgag atccgcgtgc
     1921 agaccatggc cggcgtcatc gcccccaggc tggaggccga cggccgcgtg acagtggaca
     1981 tggggcgccc ggtgctggag ccggagcggg tgcctttcga tgccaccggt ctgcagcccg
     2041 tgacccaggg tttcacacaa aaatggcctc tgacgtttga tggtcaagcg catgaagcta
     2101 ctgtttttat agtgccgata tccatgggca acccgcatgc cgtgcagctg gtggatgacg
     2161 tcgacaccgc ccctgtggcc cagaccggct cgctgatcga aaaccacccg cgctttgcgc
     2221 agcgcgtgaa cgcgggttac ttgcagatca tcagccgcgc cgaggtgcgc ctgcgcgtgt
     2281 acgagcgcgg cgccggcgag accctggcct gcggcaccgg cgcctgcgct gcggtggtgg
     2341 ccggcattgg cctgggcctg ctggacggac gcgttgacgt gcacacacgc ggcggcctct
     2401 tgagcattgc ctgggccggc ggcgtgcaag acccggtgct gatgaccggc cccgccgtga
     2461 ctgtttttga aggccagata gccattccag acctaccatg aaccattctt ccgtcacccc
     2521 catcaccgaa gacgacattg ccgatttcct ggtcaacacg ccgggttttt tcgagcgcca
     2581 cgccgagctg ctgtcggccg tcaccatctc cagcccgcat ggccagcgcg ccgtgagcct
     2641 gcaggagcgc caggccgaga tgctgcgcga gaagatcaag gggctggagc accgcgtcat
     2701 ggacatggtg cgccacggcc acgagaacac cgccatcacg cacaagatcc accaatggtc
     2761 acgcgagctg ctggcggtgc aggacgtggc cgagttgccg caggccgtgg agcagggtat
     2821 tcaccggctg ttcgacgtgc cgcaggttgc gctgcgcctg tggggcacgg ctggcatgca
     2881 tgccgatgcg cctttcaccc acggcgcgag cgaggatgtg cgctccttcg cctcgtcgct
     2941 gaccatgccg ttctgcggcc ccaacctggg cttcgaggcc acggcctggc tgccccaggc
     3001 gggccaggcg cagtcgctgg cgctgctgcc gctgcgccgc ggcgccatcg acagcgccga
//
LOCUS       DAOMTQ010000247         3020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9382_length_3020_cov_25.491062, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000247 DAOMTQ010000000
VERSION     DAOMTQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9382_length_3020_cov_25.491062"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PK518_15310"
     CDS             <1..685
                     /locus_tag="PK518_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM50325.1"
                     /translation="VAAVKGYQLILVMPDSMSIERRRLMLAYGASFDLTPKEKGMKGA
                     IARAEELKAQTPGAWIPQQFDNPANADVHARTTAQEILTDFPDGLDVLITGVGTGGHL
                     TGCARVLKERFPQLKVFAVEPALSPVISGGQPGPHPIQGLGAGFIPKNLDTSLLDGVI
                     QMDAEPAREYARRAAREEGLLVGISSGATLAAIAQKLPELPAGSRVLGFNYDTGERYL
                     SVEGFLPTA"
     gene            734..1876
                     /locus_tag="PK518_15315"
     CDS             734..1876
                     /locus_tag="PK518_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRM50326.1"
                     /translation="MTAPRHTPVRIPQTPSAAPLSAQQKKFNRHIERINEERALLSAW
                     QEAIAAYRERHAREVVPLRGSHIALLVELVQHLDAASDDKKLSKAARRTLSEGITDRV
                     ADLIDAVPDEATRAGLKDIYNRHSGGDYDAEEAEDNEVAKEMAQAMVRDLFGVDLDLQ
                     GKDTESPEDLLRRIEEQMQAQRAQAEQQREAHQAKRRAKPNARERKAQEEAAQATQSV
                     REIYRKLASSLHPDRETDPAERERKTALMQRVNQAYANNKLLDLLQLQLEIEQIDPEH
                     IAGLSEERLKHYNRVLAEQLKELQQEVNDVETHFLMEFGLDPYQRLKPAGLMRELRAD
                     LQVLERENLRLNRELNGLRADPAAIKDWIKAERQLQKQQDDFDPWF"
     gene            2008..>3020
                     /locus_tag="PK518_15320"
     CDS             2008..>3020
                     /locus_tag="PK518_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM50327.1"
                     /translation="MIRLSELRLPLAALPLDPEQHPDTALHALAAQTLGLAPEAIATL
                     RVHKRSFDARKAELLAVYIVDVALADAAQESALLAQHAGHPHIQPTPDMAWRPVGQAP
                     AGFQERPVVVGFGPCGIFAALVLAQMGFKPIVLERGKAVRERTQDTWGLWRRRELNPE
                     SNVQFGEGGAGTFSDGKLYSQIKDPRHLGRKVLQEFVAAGAPPEILYTAHPHIGTFRL
                     VKMVQELRAQIIALGGEVRFEQRVTDLLLDGRRLRGLRVLDLPSGQESELATTHAVIA
                     LGHSARDSFAMLHGHGVAMQAKPFSIGVRIEHPQSVIDRARWGKDAGHPLLGAADYKL
                     VHHA"
BASE COUNT          563 a         1067 c          977 g          413 t
ORIGIN      
        1 ggtggccgcc gtcaagggct accagctgat cctggtgatg cccgacagca tgagcatcga
       61 gcgccgccgc ctgatgctgg cctatggcgc gagcttcgac ctgacgccca aggaaaaagg
      121 catgaagggc gccatcgccc gtgccgagga actcaaggcg caaacgcccg gcgcctggat
      181 tccgcagcag ttcgacaacc cggcgaatgc cgacgtgcac gcacgcacca cggcgcagga
      241 gatcctgacc gactttcccg atgggctgga cgtgctcatc accggcgtcg gcacgggcgg
      301 gcacctgacg ggctgcgcgc gcgtgctgaa agagcgcttc ccacagctca aagtgtttgc
      361 cgtggagccc gcgctgtcgc ccgtgatctc cggcggccag cccggccccc accccatcca
      421 gggcctgggc gcgggcttca tccccaagaa cctggacacc agcctgctcg acggcgtgat
      481 ccagatggac gccgagccgg cgcgcgaata cgcccgccgc gccgcgcgcg aggaaggcct
      541 gctggtgggc atctccagcg gcgccacgct ggcggcgatt gcgcaaaaac tgcccgagct
      601 gcccgccggc agccgcgtgc tgggcttcaa ctacgacacc ggcgagcgtt acctgtcggt
      661 ggagggcttt ttgcccaccg catgacgtcg cgatggcacc ggggcgatgg cgcataggca
      721 caatcgcccc accatgaccg cgccacgcca cacccccgtc cgcatcccgc aaactcccag
      781 tgcagcgcca ctctcggcgc agcaaaagaa gttcaatcgc catatcgagc gcatcaacga
      841 ggagcgtgcg ctgctcagcg cctggcagga ggccatcgcg gcctaccgcg agcgccatgc
      901 gcgcgaagtc gtgccgctgc ggggctcgca tatcgccctt ctggtcgagc tggtgcagca
      961 cctggacgcg gccagcgatg acaaaaagct cagcaaggcc gcgcgccgca cgctcagcga
     1021 gggcatcacc gaccgcgtcg ccgatctgat cgacgccgtg cccgatgagg ccacgcgcgc
     1081 cggcctcaag gacatctaca accgccacag cggcggcgac tacgacgccg aagaggccga
     1141 ggacaacgag gtggccaagg agatggccca ggccatggtg cgcgacctgt ttggcgtgga
     1201 tctggatctg cagggcaagg acacggagtc gcccgaggac ctgctgcgcc gcatagaaga
     1261 acagatgcag gcccagcgcg cccaggccga gcagcagcgc gaggcacacc aggccaagcg
     1321 ccgtgccaag cccaacgcac gcgagcgcaa ggcgcaggaa gaggccgccc aggccacgca
     1381 gtcggtgcgc gagatctacc gcaagctcgc cagcagcctg cacccggacc gcgaaaccga
     1441 ccccgccgag cgcgagcgca agacggcact aatgcagcgc gtcaaccagg cctacgccaa
     1501 caacaagctg ctcgacctgc tgcaactgca gctggagata gagcagatcg accccgagca
     1561 catcgccggc ctgagcgagg agcgcctgaa acactacaac cgcgtgctgg ccgagcagct
     1621 caaggagctg cagcaggagg tgaacgacgt ggaaacccat tttttgatgg agttcgggct
     1681 cgacccctac cagcgcctca aacccgctgg gctgatgcgc gagctgcgcg ccgatctgca
     1741 ggtgctggag cgcgaaaacc tgcgtctgaa cagggagctg aacgggctgc gcgccgaccc
     1801 ggccgccatc aaggactgga tcaaggccga gcgtcagctg caaaagcagc aggacgattt
     1861 cgacccgtgg ttttgacagc atccgtgtta ctatttattt gatagctgtt tacgcttacc
     1921 cacaaagcgc tacagcccta tttgattcaa gtttatcggg gccatggtgc taccatggcg
     1981 cccccttttg cttgatagac ccaagccatg atccgcctgt ccgaactgcg cctgcccctg
     2041 gccgccctgc cgctcgatcc cgagcagcac cccgacaccg ccctgcacgc cctggccgcc
     2101 cagaccctgg gcctggcgcc cgaggccatc gccacgctgc gggtgcacaa gcgcagcttt
     2161 gacgcgcgca aggccgagct gctggccgtg tacatcgtcg atgtagccct ggccgatgcc
     2221 gcgcaggaat ccgccctgct ggcgcagcac gccggccacc cgcatatcca gcccacgccc
     2281 gacatggcct ggcgccccgt gggccaggcg ccggcaggct ttcaagaacg cccggtggtg
     2341 gtgggctttg gcccctgcgg catctttgcc gcgctggtgc tggcgcagat gggcttcaag
     2401 cccatcgtgc tggagcgcgg caaggccgtg cgcgaacgca cccaggacac ctggggcctg
     2461 tggcgcaggc gtgagctcaa tcccgagtcc aacgtgcagt ttggcgaggg tggcgccggc
     2521 acattttccg acggcaagct ctacagccag atcaaggacc cgcgccacct ggggcgcaag
     2581 gtgctgcagg agttcgtcgc cgccggcgcc ccgcccgaaa tcctgtacac cgcccacccg
     2641 catatcggca ccttccgcct ggtgaagatg gtgcaggagc tgcgcgcgca gatcatcgcc
     2701 ctgggcggcg aggtgcgctt tgagcagcgc gtgaccgacc tgctgctgga cggccggcgc
     2761 ctgcgcggcc tgcgcgtgct ggacctgcca agcggacagg agagcgagct ggccaccacg
     2821 cacgccgtca tcgccctggg ccacagcgcg cgcgacagct ttgccatgct gcacgggcat
     2881 ggcgtggcca tgcaggccaa gccgttctcg ataggcgtgc gcatcgagca cccacaaagc
     2941 gtgatcgacc gcgcgcgctg gggcaaggac gcgggccacc cgctgctggg cgcggccgac
     3001 tacaagctgg tgcaccacgc
//
LOCUS       DAOMTQ010000248         3008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9429_length_3008_cov_11.803590, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000248 DAOMTQ010000000
VERSION     DAOMTQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9429_length_3008_cov_11.803590"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PK518_15325"
     CDS             complement(<1..746)
                     /locus_tag="PK518_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM50328.1"
                     /translation="MTAQPSPELRVSGLAHAFGPRAVLEDIHLHLPAGRTLALVGPSG
                     SGKSTLLHLCAGLLTLRDGRIANGFARTTMLFQQPHLLPWKSALDNIALGLKARGMAR
                     GARLQAAHAMGRAMGLDELALAQFPPQLSGGMQSRVALARALVLEPDLLLLDEPFSAL
                     DIGMRQQMHRLLLAEQQRRPLAVLMITHDVMEAVALADQVLVLAGTPARLRWRLDLAQ
                     PAARRDDAWVHRQTARLLAQLLAHGCPEEV"
     gene            complement(743..1555)
                     /locus_tag="PK518_15330"
     CDS             complement(743..1555)
                     /locus_tag="PK518_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM50329.1"
                     /translation="MALLRNWLLGIPRYLWGGWGAIASLLLLVALWEALAAAYGPLVL
                     PTPLQTAHMLGQWMAQGGELWPALAVTARRAFIGLALAVLAGSLLGVAAGMSMTASMM
                     ARPWITVLLGTPPIAWLVLAMLWFGSGDGTPVFTVFIACLPVVFVGGMQGARTLERHW
                     RDLARAYRLPWHMRVLDVYMPHVVSYLFPAWIVALGSSWKVVVMAELLSSDTGVGAEL
                     ATSRAQLDMAATLAWIGAVVLVLLALEYLLLEPIKREIERWRDSAGTAEERA"
     gene            complement(1555..2550)
                     /locus_tag="PK518_15335"
     CDS             complement(1555..2550)
                     /locus_tag="PK518_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM50330.1"
                     /translation="MRWPTAPAAPLRAQPGGGTAPARRPRLTLSGPFAAVSYPLMRIA
                     DSGALADMADKVEFSSWKTPDQLRALAIEQSADLVAMPSNVAANLFNRGVPLALLDIS
                     TWGLLWMVSREAGLRTLADFRGKEVVMPFRGDMPDILFRLLAERQGIAVGKDIRLRYV
                     ASPIDAMQLLITRRADHALLAEPAVSMGLRKSQSFPVKVIAPELHRSVDLQQEWGRVL
                     GRAPRIPQAGITALGRVQGDAALLARFEQEHAKALAWCQAQPEACGEMAARHVEMLTP
                     EGVADAMRATQSTMTLVGASQARAELEFFYQQLLERQPGLVGGKLPPAAFYAGRA"
     gene            2549..>3008
                     /locus_tag="PK518_15340"
     CDS             2549..>3008
                     /locus_tag="PK518_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50331.1"
                     /translation="MWRASAPVQIDKTAESVAEIKREMAVFFGGTRGTTAEELARQQK
                     GLTLSLPGAYETASAVMGTIGGIVRYGRPDDYVFKRKAEIEAMTPAQANGAAKALDPN
                     ALTWVIVVPSPPETDWPLTSSKPVMPAAASAKTAAELARIGFQGTRRPDRP"
BASE COUNT          481 a         1116 c         1044 g          367 t
ORIGIN      
        1 tgcacctcct ccgggcagcc gtgcgcgagc agctgcgcca gcagccgcgc cgtctggcgg
       61 tgcacccagg cgtcgtcgcg gcgcgcggcg ggctgggcca gatccagacg ccagcgcaga
      121 cgcgcgggcg ttccggccag caccagcacc tggtcggcca gcgccacggc ctccatcacg
      181 tcgtgcgtga tcatcagcac ggccagcggc cggcgctgct gctcggccag cagcaggcgg
      241 tgcatctgct gcctcatgcc gatatcgagc gcggagaacg gctcgtcgag cagcagcagg
      301 tcgggctcca gcaccagcgc gcgcgccagc gccacgcggc tttgcatgcc gcccgagagc
      361 tgcggcggaa actgcgccag cgccagctca tcgagcccca tggcccggcc catggcatgc
      421 gcggcctgca ggcgcgcgcc acgcgccatg ccgcgcgcct tcaggccgag ggcaatgttg
      481 tccagcgcgc tcttccaggg caacaggtgc ggctgctgga acagcatggt ggtgcgcgca
      541 aagccgttgg cgatgcgccc gtcacgcagc gtcagcaggc cggcgcacag gtgcagcagc
      601 gtgctcttgc cgctgcccga ggggccgacc agcgccagcg tgcggccggc cggcagatgc
      661 aggtggatgt cttcgagcac ggcgcgcggg ccgaaggcat gcgccaggcc gctcacgcgc
      721 agctcggggc tgggctgtgc cgtcatgcgc gctcctcggc cgtgccagcg ctgtcgcgcc
      781 agcgttcgat ctcgcgcttg atgggctcca gcagcaggta ttccagcgcc agcaacacca
      841 gcaccacagc tcctatccag gccagcgtcg ccgccatgtc cagctgtgcg cggctggtag
      901 ccagctcggc gccaacgccc gtgtcggacg acagcagctc ggccatcacc accaccttcc
      961 agctgctgcc cagcgcgacg atccaggccg ggaacaggta ggacaccaca tgcggcatgt
     1021 acacgtcgag cacgcgcata tgccagggca accgataggc gcgcgccagg tcgcgccagt
     1081 ggcgctccag cgtgcgcgcg ccctgcatgc cgccgacaaa gaccaccggc aggcaggcaa
     1141 tgaacacggt gaacaccggc gtgccatcgc cgctgccgaa ccacagcatg gccagcacca
     1201 gccaggcgat gggcggcgtg cccagcagca ccgtgatcca ggggcgcgcc atcatcgacg
     1261 ccgtcatcga catgccggcg gccacgccca gcaggctgcc ggccagcacc gccagcgcca
     1321 gcccgatgaa ggcgcgcctg gccgtcaccg ccagcgccgg ccacagctcg ccgccctgcg
     1381 ccatccactg gccgagcatg tgcgccgtct gcaacggcgt cggcagcacc agcggcccgt
     1441 aggcggcggc cagcgcctcc cacagggcca ccagcagcag caggctggcg atcgcgcccc
     1501 agccacccca gagatagcgc ggaatgccca gcagccagtt gcgcaacaag gccatcaggc
     1561 gcgtcccgcg tagaacgcgg ccggcggcag cttgccgccg accaggccag gctgccgctc
     1621 cagcagctgc tggtagaaga actccagctc ggcgcgcgcc tggctggcgc ccaccagggt
     1681 catggtggat tgggtcgcgc gcatggcgtc ggccacgccc tcgggcgtca gcatctcgac
     1741 atggcgcgcc gccatctcgc cgcaggcctc gggctgggcc tggcaccagg ccagcgcctt
     1801 ggcgtgctcc tgctcgaagc gcgccagcag cgccgcatcg ccctgcaccc ggcccagcgc
     1861 cgtgatgccg gcctgaggaa tgcgcggggc gcgccccagc acgcggcccc actcctgttg
     1921 cagatcgacg ctgcggtgca gctcgggggc aatcaccttc accgggaacg actggctctt
     1981 gcgcagcccc atggataccg ccggttcggc cagcagcgca tggtcggcgc ggcgcgtgat
     2041 gagcagctgc atggcgtcga tgggcgaggc cacatagcgc agccggatgt ccttgcccac
     2101 ggcgatgccc tggcgctcgg ccagcaggcg aaagaggatg tcaggcatgt cgccgcgaaa
     2161 cggcatcacc acttccttgc cgcgaaagtc cgccagggta cgcaggcccg cctcgcgcga
     2221 gaccatccac agcagacccc aggtgctgat gtccagcagc gccagtggca cgccgcggtt
     2281 gaacaaattg gctgccacat tgctgggcat ggccaccagg tcggcggatt gctcgatggc
     2341 cagcgcgcgc agctgatccg gcgttttcca gctgctgaac tccaccttgt cggccatgtc
     2401 ggccagcgca ccgctgtcgg caatgcgcat caaggggtag gacacggcgg cgaacgggcc
     2461 ggacagcgtc agcctggggc gccgcgccgg tgccgtgccg cccccgggct gcgcgcgcag
     2521 cggcgccgcc ggtgcggtcg gccagcgcat gtggcgcgcc tcggcgccgg tgcagatcga
     2581 caagaccgcc gagtcggtgg ccgagatcaa gcgcgagatg gccgtgttct tcggcggcac
     2641 gcgcggcacc actgccgagg aactggcgcg ccagcagaag ggcctgaccc tgagcctgcc
     2701 gggcgcgtac gaaaccgcca gcgccgtgat gggcaccatc ggcggcatcg tccgctacgg
     2761 ccgcccggac gactacgtgt tcaagcgcaa ggccgagatc gaggcgatga ccccggccca
     2821 ggccaatggg gcggcgaagg cgctggaccc gaacgcgctg acctgggtga tcgtcgtgcc
     2881 ctcgcccccg gagacggact ggccgctgac cagctcgaag ccggtgatgc cggcggcagc
     2941 cagtgcgaag acggcggccg agctggccag gatcggcttc cagggcacgc gtcgcccgga
     3001 ccgacccg
//
LOCUS       DAOMTQ010000249         2995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9492_length_2995_cov_24.640816, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000249 DAOMTQ010000000
VERSION     DAOMTQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_2995_cov_24.640816"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PK518_15345"
     CDS             complement(<1..874)
                     /locus_tag="PK518_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRM50332.1"
                     /translation="MTPITLKTYQQTALDTLAAFARAARVKGPALAFGELAGRPWHAG
                     AFGDGLPCVCLRIPTGGGKTVLAAHAVPLLAREWAAVDAPVAVWLVPSDAIRQQTLKA
                     LQTPGHPYRAALADAYGEGLRVCALEDVAQIAPPEWGRNAVVVVATIQSFRIEDAGQR
                     NVYSFSESFEPHFRGADERALAGLRDLPDALVTPEDVAADTTGVLAGFAGQPRWSLAN
                     WLALHRPILIVDEAHNAKTDKSFTALQRLNPGFILELTATPIAAKTNVLYHVSAQELA
                     AEDMIKLPIVLAEHP"
     gene            complement(871..2766)
                     /locus_tag="PK518_15350"
     CDS             complement(871..2766)
                     /locus_tag="PK518_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM50333.1"
                     /translation="MNAQELLESLNLLDENERIEAKRAQEAGKSLLETICAFANEPHL
                     GGGWLLLGVAPDEQALFPGYAVEGVPQPDKLSADLASQCTTAFNRPLRVEIRTETLAG
                     EPVVVVFVPEAAPQDKPIYFKATGLPKGAFRRIGSTDQRCTEDDLETLYQSRQRESFD
                     AGLVSGAELDDFDDAAIAEYRQARAEANPDAEELRWTDAELLQALNAVRRDAAGAWKP
                     TVAGLLLFGKSVALRRCFPMTRVDYIRVPGREWVPHPDRRFDTVELRAPLFTLLRRTQ
                     AAVLDDLPKGFGLEEGQLQRSDTPVIPLRALREALVNALMHRSYRVQAPVQVIRYANR
                     LEIRNPGFSLKSPDHLGEPGSMPRNPHIAAVLHETRFAETKGSGIRAMREAMDKAGLV
                     PPLFESDRGQDQFSTLFFFHHFLGEDDIRWLAQFKALHLSDEEARALVVAREAGAIDN
                     ATYRALNKVDTLAASNALRRLRDAGLFSQQGRGSATWYQPTDRLLGGAPGGLPGQLDA
                     LSSKSAPLSSMPEGLSSKSEGLSSKSDEERNALLDEIPGQLAARIGSIGRRHPPEPVR
                     ELVVELCRLRAWRAEELALLLSRKPETIRQDYLRPLLAQKRIAMTQPDKPKSPQQAYR
                     SVQESLP"
     gene            complement(2772..>2995)
                     /locus_tag="PK518_15355"
     CDS             complement(2772..>2995)
                     /locus_tag="PK518_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRM50334.1"
                     /translation="YYLLFNGILGDKRPASGNVLTRAVLEALLQLHAATPHPEAPLVV
                     YGEANRLGPETLARARVTFKHIPYDVKAR"
BASE COUNT          456 a         1040 c         1008 g          491 t
ORIGIN      
        1 cggggtgctc ggccagcacg atgggcagct tgatcatgtc ctcggccgcc agctcctgcg
       61 cgctgacgtg gtacagcacg ttggtcttgg cggcgatggg cgtggccgtc agctccagga
      121 tgaagcccgg attcagccgc tgcagcgcgg tgaagctctt gtccgtcttg gcgttgtgcg
      181 cctcgtccac gatgaggatg ggccggtgca gcgccagcca gttcgccagg ctccagcgcg
      241 gctggcccgc aaagccggcc agcacgccgg tggtgtcagc cgctacatcc tcgggcgtga
      301 ccagcgcgtc gggcagatcg cgcagccctg ccagcgcccg ctcgtccgcg ccccggaaat
      361 gcggctcgaa gctctcggaa aagctataca cattgcgctg gcccgcgtcc tcgatgcgaa
      421 agctctggat cgtcgccacc accacaacgg cattacggcc ccactcgggc ggcgcgatct
      481 gcgccacgtc ttcgagcgcg cacacgcgca gcccctcgcc gtaggcatcg gccagcgccg
      541 cgcggtacgg gtggccgggc gtctgcagcg ccttcagggt ttgctggcga atcgcatcgc
      601 tgggcaccag ccacacggcc accggcgcat ccaccgccgc ccactcgcgc gccagcagcg
      661 gcacggcgtg cgcggccagc accgtcttgc cgccaccggt ggggatgcgc aggcacacgc
      721 agggcagccc gtcgccaaac gccccggcgt gccagggccg gcccgccagc tcgccaaagg
      781 ccagcgccgg gcccttgacc cgcgccgcgc gggcgaaggc ggccagggtg tcgagggcag
      841 tttgctggta ggtcttgagg gtgatggggg tcatggaagg ctttcctgca cgctgcggta
      901 tgcctgctgc ggcgacttgg gcttgtccgg ctgagtcatg gcaatgcgct tttgcgccag
      961 cagcggacgc aggtaatcct gccggatggt ctcaggcttg cggctcagca acagggccaa
     1021 ttcttcggcc cgccatgccc gcaggcggca aagctccacc accagttctc gcaccggctc
     1081 cggcggatgg cgccgaccta tgctgccgat gcgcgccgcc aattgacctg gaatttcatc
     1141 cagcagcgca ttccgttcct cgtccgactt gctggataaa ccctcagact tactggataa
     1201 gccctcaggc atgctggata agggggctga cttactggat agcgcatcca gctggccagg
     1261 caagccgcca ggcgcgccgc ccaacagcct gtccgtgggc tggtaccaag tggccgagcc
     1321 ccggccttgc tggctgaaca ggcccgcgtc gcgcagccgc cgcagggcgt tgctggcggc
     1381 cagggtgtcc accttgttca gggcgcggta ggtcgcgttg tcgatggcgc ccgcctcgcg
     1441 cgccacaacc agggcgcggg cctcttcgtc gctgaggtgc agcgccttga actgcgccag
     1501 ccagcggatg tcgtcctcgc ccagaaagtg gtgaaagaaa aacagggtgc tgaactgatc
     1561 ctgcccccgg tcagactcga acaggggcgg caccagcccg gccttgtcca tcgcctcgcg
     1621 catggcgcga atgccgctgc ccttggtttc ggcaaagcgg gtttcgtgca gcacggcggc
     1681 gatgtggggg ttgcgcggca tcgagcccgg ctcgcccaga tggtctggcg acttgagcga
     1741 aaagccgggg ttgcggattt cgagccggtt ggcatagcgg atcacctgca ccggcgcctg
     1801 cacgcggtag ctgcggtgca tcagggcatt gaccagcgcc tcgcgcaggg cgcgcagcgg
     1861 gatgacgggc gtgtcgctgc gctgcaactg gccttcttcc agcccaaagc ccttgggcaa
     1921 gtcatccagc accgcggcct gggtgcggcg cagcagcgtg aacagcggcg cgcgcagctc
     1981 caccgtgtca aagcgccggt cgggatgcgg cacccattcg cgcccaggca cccggatgta
     2041 atccacccgc gtcatcggga agcagcgccg caaggcaacg gacttgccaa acagcaacaa
     2101 cccggccacc gtgggcttcc atgcgcctgc agcatcccgc cgcaccgcgt tcagcgcctg
     2161 caagagttct gcgtccgtcc agcgcagctc ctcggcatcg ggattggcct cggcccgggc
     2221 ctggcggtac tcggcaatgg cggcgtcgtc gaaatcatcc agctcggcgc cagaaaccag
     2281 ccccgcgtca aagctctcgc gctggcgcga ctggtacagc gtttccagat cgtcctcggt
     2341 gcaccgctgg tcggtgctgc cgatgcggcg aaatgcgccc ttgggcaggc cggtggcctt
     2401 gaagtaaatg ggcttgtcct gcggcgccgc ctcgggcacg aacaccacca cgacgggttc
     2461 gcccgccagc gtttcggtgc ggatctccac ccgcagcggc ctgttgaatg ccgtggtgca
     2521 ctggctggcc aggtcggcgc tgagcttgtc cggctggggc acgccctcca ccgcgtagcc
     2581 gggaaacagc gcctgttcat ccggcgccac gcccagcaac agccaaccgc cgcccaggtg
     2641 gggttcattg gcaaaggcgc aaatggtttc cagcagcgac ttgcccgcct cctgcgcgcg
     2701 cttggcctcg atgcgctcgt tttcatcgag caggttcagg ctttccagca gctcctgtgc
     2761 gttcatcccc ctcacctcgc cttcacgtcg tacggaatgt gcttgaacgt gacccgcgcg
     2821 cgcgccagcg tctcggggcc gaggcggttg gcctcgccgt agaccaccag cggcgcctcg
     2881 gggtggggcg tggcggcgtg tagttgcagc agtgcctcca gcaccgcgcg cgtgagcacg
     2941 ttgccgctgg cggggcgctt gtcgcccagg atgccgttga acagcaggta gtagg
//
LOCUS       DAOMTQ010000250         2994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9501_length_2994_cov_25.892140, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000250 DAOMTQ010000000
VERSION     DAOMTQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9501_length_2994_cov_25.892140"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2555)
                     /gene="ppc"
                     /locus_tag="PK518_15360"
     CDS             complement(<1..2555)
                     /gene="ppc"
                     /locus_tag="PK518_15360"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRM50335.1"
                     /translation="MTAPARKTVEAAPAAAPARKIDKDLPLIQDIRLLGRILGDVIRE
                     QEGVAAYEVVEQVRKLSVAFRRDADQEADRALKKLLKSLTGDQTVSVIRAFTYFSHLA
                     NLAEDRHHIRRRAVHERAGSSQEGSIEVALARLRWAGIAPRTVAQTLATSYVAPVLTA
                     HPTEVQRKSILDAEREIAQLLTQRDDIQARAQLYNSAKDALTPRELAANEAQLRARVA
                     QLWQTRLLRYSKLTVADEIENALSYYESTFLREIPRIYADLERELGGNQAVASFLRMG
                     QWIGGDRDGNPNVSADTLALALRRQAEVALRHHLTEVHNLGRELSLSSNLVQITPEMR
                     ALAERSPDTSEHRSDEPYRRALTGIYARLAATLTELTGGKAALHAVAPQNPYASAAEF
                     LADLRVIEASLHSHRGAVLTAERLHPLMRAVEVFGFHLATVDLRQSSDQHEKVVAELL
                     AKARVHADYAALPEAERRALLMTLLCDARPLRVVGAQYSELATSEIAIFETARQMRER
                     YGADAIRHYIISHTETVSDLLEVLLLQKEVGLMQGQLDAGARADLIVVPLFETIEDLR
                     NAAPIMREFYQLPGIAQLVRAGGGEQDIMLGYSDSNKDGGIFTSNWELYRAEIALVAL
                     FDELNAGQAGAPIRLRMFHGRGGTVGRGGGPSYQAILAQPPGTVRGQIRLTEQGEVIA
                     SKYANPEIGRRNLETLVAATLEATLLQPTKPATKAFLEAAGQLSQASMAAYRALVYET
                     PGFTDYFFNSTPIREIAQLNIGSRPASRKANQRIEDLRAIPWGFSWGQCRLTLPGWFG
                     FGAAVQAFMNEPGKEPKAQLALLQKMYRQWPFFRTLLSNMDMVLAKSDLALASR"
     gene            2666..>2994
                     /locus_tag="PK518_15365"
     CDS             2666..>2994
                     /locus_tag="PK518_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRM50336.1"
                     /translation="MTTARALPPIVIATRESQLALWQAEHVQALLTARGHSVSLLGMT
                     TRGDQILDRTLSKVGGKGLFVKELEVALEEGRADIAVHSLKDVPMELPTGFSLACVME
                     REDPRDA"
BASE COUNT          438 a         1014 c         1032 g          510 t
ORIGIN      
        1 cgcgaggcca gggccaggtc gctcttggcc agcaccatgt ccatgttgga cagcaacgtg
       61 cgaaagaacg gccactgccg gtacatcttc tgcagcagcg ccaactgcgc cttcggctcc
      121 ttgcccggct cgttcatgaa ggcctgcacc gccgcgccaa agccgaacca gcctggcagc
      181 gtcaggcggc actggcccca gctgaagccc cagggtatgg cgcgcaagtc ctcgattctc
      241 tggttggcct tgcgcgatgc gggacgcgag ccgatgttga gctgggcgat ctcgcgtatc
      301 ggagtggagt tgaagaaata gtcggtaaag ccgggcgtct catacaccag cgcgcggtag
      361 gccgccatgc tggcctgcga cagctgcccg gccgcctcca ggaaggcctt ggtggccggt
      421 ttggtgggct gcagcagcgt ggcctccagc gtggcggcga ccagggtctc gaggttgcgc
      481 cggccgatct cagggttggc gtatttgctg gcaatcacct cgccctgctc cgtcagccgg
      541 atctggccgc gcacggtgcc cggcggctgc gccaggatgg cctggtagct ggggccgccg
      601 ccgcggccca cggtgccgcc acggccgtgg aacatgcgca ggcgtatcgg cgcaccggcc
      661 tggccggcgt tcagctcgtc gaacagcgcc accagcgcga tctcggcgcg gtacagctcc
      721 cagttgctgg tgaagatgcc gccgtccttg ttgctgtcgc tgtagcccag catgatgtcc
      781 tgctcgccgc cgcccgcgcg caccagctgc gcgatgcccg gcagttggta gaactcgcgc
      841 atgatgggcg cggcattgcg cagatcctct atggtttcga acagcggcac gacgatcagg
      901 tcggcgcgcg cgccggcatc cagctggccc tgcatcaggc ccacctcttt ttgcaaaagc
      961 agcacctcca gcaggtcgct cacggtctcg gtgtggctga tgatgtaatg acggatcgcg
     1021 tcggcaccat agcgctcgcg catctgccgc gcggtctcga agatggcgat ctcacttgtc
     1081 gccagctcgg aatattgcgc gcccaccacg cgcagcggcc gcgcatcaca cagcagcgtc
     1141 atcagcagcg cacggcgctc ggcctcgggc agggcggcat agtcggcatg cacgcgcgcc
     1201 ttggccagca gctcggccac caccttctcg tgctgatccg agctttggcg caagtccacc
     1261 gttgccaggt gaaagccgaa cacctccacg gcacgcatca gggggtgcag gcgctcggcc
     1321 gtgagcaccg cgccgcggtg cgagtgcagc gaggcctcga tgacgcgcag atcggccaga
     1381 aactccgcgg cactggcata ggggttttgc ggcgccacgg catgcagcgc ggccttgccg
     1441 ccggtcagct cggtcagcgt ggcggccagg cgcgcataga tgccggtgag cgcgcggcgg
     1501 tagggctcgt cgctgcggtg ttcgctggtg tcgggtgagc gctcggccag ggcacgcatc
     1561 tcgggggtga tctgtaccag gttgctggac agcgacagct cgcggcccag gttgtgcacc
     1621 tcggtcaggt ggtggcgcag cgccacctcg gcctggcggc gcagggccag ggccagggtg
     1681 tcggcactga cattggggtt gccgtcgcgg tcgccgccta tccactggcc catgcgcaga
     1741 aagctggcca cggcctggtt gccgcccagc tcgcgctcca ggtcggcgta gatgcgcggt
     1801 atctcccgca aaaaagtgga ttcgtagtac gacagcgcgt tctcgatctc gtcggccacc
     1861 gtgagcttgg agtagcgcag cagccgcgtc tgccacagct gggccacgcg ggcgcgcagc
     1921 tgggcctcgt tcgcagccag ctcacgcggc gtgagcgcgt ccttggcgct gttgtagagc
     1981 tgggcgcgcg cctgtatgtc gtcgcgctgg gtgagcagct gggcgatctc gcgctcggcg
     2041 tccaggatgc tcttgcgctg cacctcggtg gggtgggccg tgagcaccgg tgccacatag
     2101 ctggtggcca gggtctgggc cacggtgcgc ggcgcaatgc cggcccagcg caggcgcgcc
     2161 agcgccacct cgatgctgcc ctcctggctg ctgccggcgc gctcatgcac ggcccggcgg
     2221 cggatatggt gccgatcctc ggccaggttc gccaggtgac tgaaataggt gaaggcgcgg
     2281 atcacgctca ccgtctggtc gcccgtcagg ctcttgagca gttttttcag cgcgcgatcc
     2341 gcctcctggt cggcgtcgcg ccgaaaggcc accgagagct tgcgcacctg ctccaccacc
     2401 tcgtaggcgg ccacgccctc ctgctcgcgg atcacgtcgc ccaggatgcg cccaagcagg
     2461 cgaatgtcct ggatcagcgg cagatccttg tcgatcttgc gtgccggggc tgcggcaggc
     2521 gcggcttcta ctgtcttgcg tgcgggagcg gtcatgcgcg ggtgtctcct gatgcgcctg
     2581 tgggtatggc gggtgctgca gcgcagcatg ctagcatccc ctgctgcccc aagccgtgtc
     2641 cacggggccc gaaaaaactc tccccatgac cactgcccgc gccctccccc ccatcgtcat
     2701 cgccacgcgc gaaagccagc ttgccctgtg gcaggccgag catgtgcagg ccctgctcac
     2761 cgcgcgcggc cacagcgtga gcctgctggg catgaccacg cgcggcgacc agatcctcga
     2821 tcgcacgctg tccaaggtcg gcggcaaggg cttgttcgtc aaggaactgg aggtggcgct
     2881 ggaggaaggc cgggccgaca tcgccgtgca ttcgctcaag gatgtgccga tggagctgcc
     2941 caccggcttt tccctggcct gcgtgatgga gcgcgaagac ccgcgcgacg cctt
//
LOCUS       DAOMTQ010000251         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9539_length_2983_cov_25.148224, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000251 DAOMTQ010000000
VERSION     DAOMTQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9539_length_2983_cov_25.148224"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="PK518_15370"
     CDS             complement(<1..1808)
                     /locus_tag="PK518_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM50337.1"
                     /translation="MARPQHPLGISGRIAQAFQAAQITPLLALLALLLGLFAVLVTPR
                     EEEPQIDVTMANVIVPFPGASVADVEQLIATPAEQVLSQMLGVEHVTSVSHPGVAVLT
                     VQFKVGVQRNDALVRLHDTLRSHADWLPRGLGAMEPIIRPKGIDDVPVVTLTLYSEQD
                     DTGAYDLERVAHSMEAELMRVDGARTVSTIGGPGRAVRVQLDAARMAGRGVTVDDIRA
                     TLQSANQGLPVGELLGGNQSVAIEAGPFLAHAREVGELVVSSRGGKPVFLQDVAQVED
                     GPLPAQRYVWYAGVKDGQASEYPAVTLAVTKKAGANAIAVARAVMQRVQDLRGTVIPA
                     AVQVAETRDYGATANDKAQKLIQKLLFATVSVVALVFVALGRREAVIVGVAVGLTLAA
                     TLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHQSLYPEKTLTELIPGAV
                     DEVGGPTILATFTVIAALLPMAFVTGLMGPYMAPIPINASMGMLISLAVAFTVTPWLA
                     RLWMKSHGSQSQAAQEHTGLAARLAPLFTRIFTPLLDDTRGRRNRTLLGLAVAGLIAL
                     SVLLPMIGWVQLKMLPFDNKSEFQVIVDMPAGTPPEA"
     gene            complement(1810..2853)
                     /locus_tag="PK518_15375"
     CDS             complement(1810..2853)
                     /locus_tag="PK518_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRM50338.1"
                     /translation="MSLAPWVLSVLTVGLAAQAHVHASEAAAAPGYYTVQATGSTGWS
                     SMDAKVEAVRETLLAAQVPGAIVALPVKAGDAVKAGQELARIDARMASQGAAASSAQV
                     AAAQAGLNVATKEYERQKQLFAKRYISQAALDGAEAQWRAAQAQVTALQAQAGVAATQ
                     TGLHSVRAPYSGIVSSVPVNLGDMAMPGRPLLSLYDPAALRLTAQLTQDQARQLRSGV
                     QVEIPGLTVQRLSIAASQIQLLPTADPLSHTVAVRVQLPANQQGAMPGMFARLWWQGD
                     AADAAQRVLIPSTAVVRRAEMTGVYVQGDNGKPQLRQVRLGLPQGEMVEVLSGLRVGD
                     QVAVEPQAAARVR"
BASE COUNT          488 a         1046 c          985 g          464 t
ORIGIN      
        1 gcctcgggcg gcgtgccggc gggcatgtcg acgatgacct ggaactcgga tttattgtcg
       61 aacggcagca tcttgagctg cacccagccg atcatgggca gcagcaccga cagggcgatc
      121 aggcccgcca cggccaggcc cagcagcgtg cggttgcgcc gcccgcgtgt gtcatccagc
      181 agtggggtga agatgcgcgt gaacagcggc gccagcctgg ccgccaggcc ggtgtgctcc
      241 tgtgcggcct gggactggct gccatggctt ttcatccaca gccgcgccag ccagggcgtg
      301 acggtgaagg ccacggccag cgagatcagc atgcccatgc tggcgttgat gggaatcggc
      361 gccatgtacg gccccatcag cccggtgacg aaggccatgg gcagcagggc ggcaatcacc
      421 gtgaaggtgg ccaggatggt cggcccgccg acttcatcga ccgcgccggg gatcagctcg
      481 gtcagcgtct tttctggata gagagactga tggcgatgga tgttctccac cacgacgatg
      541 gcatcgtcca ccaggatgcc gatggagaag atcagcgcga acagcgacac gcggttgagc
      601 gtgaaacccc aggcccagga ggcgaacagc gtggccgcca gcgtcaggcc cacggccacg
      661 ccgacgatga cggcctcgcg ccggcccagc gcgacaaaca ccagggccac gacggagacg
      721 gtggcaaaca gcagcttctg gatcagcttc tgcgccttgt cgttggccgt agcgccgtag
      781 tcgcgcgtct cggctacctg cacggcggcg gggatgacgg tgccgcgcag atcctgcacg
      841 cgctgcatta cggcacgcgc cacggcgatg gcgttggcgc cggccttctt cgtcacggcc
      901 agggtcacgg ccgggtattc gctggcctgg ccgtccttca caccggcgta ccagacatag
      961 cgctgagcgg gcagggggcc gtcttccacc tgggccacgt cctgcaaaaa cactggcttg
     1021 ccgccacggc tgctgaccac cagctcaccc acttccctgg cgtgggccag gaacggcccg
     1081 gcctctatgg ccacggactg gttgccaccc agcagctcgc ccacgggcag gccctggttg
     1141 gcggactgca gcgtggcgcg gatatcgtcc accgtcaccc cgcgcccggc catgcgcgcc
     1201 gcatcaagct gcacgcgcac ggcgcggccc ggcccgccga tggtgctcac ggtgcgcgcg
     1261 ccatccacac gcatcagctc agcctccatg ctgtgcgcca cgcgctccag gtcgtaggcg
     1321 ccggtgtcat cctgctcgct gtacagcgtc agcgtgacca cgggcacgtc gtctatgccc
     1381 ttggggcgga tgatgggctc catcgcgccc aggccccgcg gcagccagtc ggcgtggctg
     1441 cgcagggtgt cgtgcaggcg caccagcgcg tcgttgcgct gcacccccac cttgaactgc
     1501 accgtgagca ccgccacacc ggggtgcgag acgctggtca catgctccac gcccagcatt
     1561 tgcgagagca cctgctcggc cggcgtggcg atcagctgct ccacgtcggc caccgaggca
     1621 ccagggaagg gcacgatgac gttggccatg gtcacgtcga tctgcggctc ctcctcgcgc
     1681 ggcgtgacca acacggcaaa cagcccgagc agcagcgcca gcagcgccaa cagcggcgtg
     1741 atctgcgccg cctgaaaggc ctgggcgatg cggcccgaga tgcccagggg gtgttgtgga
     1801 cgggccatct cagcgcaccc tggccgcggc ctgcggctcc acggccacct ggtcgcccac
     1861 gcgcaggccg ctcagcacct ccaccatctc gccctggggc aggcccaggc gcacctggcg
     1921 cagctgcggc ttgccgttgt cgccttgcac atagacgccc gtcatctcgg cacggcgcac
     1981 gactgccgtg gacggtatca gcacccgctg agctgcatcc gcggcatcac cctgccacca
     2041 caggcgcgca aacatgcctg gcatggcgcc ctgctgattg gccggcagct gcacgcgcac
     2101 cgccacggtg tggctcaagg ggtcggccgt gggcagcagc tggatttgag acgctgcaat
     2161 cgacaggcgt tgcacggtca atcctggaat ttccacctgc acacctgaac gcagttgccg
     2221 ggcttgatcc tgcgtcagct gcgccgtcag gcgcagagca gcggggtcgt acaaggacag
     2281 cagcggccgg cccggcatgg ccatgtcgcc cagattgacg ggcacgctgg agacaatgcc
     2341 ggaatagggt gcgcgtacgc tgtgcagccc ggtttgcgtg gccgccacac cggcctgtgc
     2401 ctgcagcgcg gtcacctgcg cctgggctgc acgccactgc gcctcggcgc catcgagcgc
     2461 cgcctggctg atgtagcgct tggcaaacag ctgcttctgg cgttcgtact ccttggttgc
     2521 cacgttcagg ccggcctggg ctgcggccac ctgtgcgctg ctggccgccg cgccttgact
     2581 ggccatgcgc gcgtcgatac gcgccagctc ctgcccggct ttcacggcgt caccggcctt
     2641 gaccggcagg gccacgatgg cgcccggcac ctgggccgcg agcagcgtct cgcgcaccgc
     2701 ctcgaccttg gcatccatgc tgctccagcc ggtgctgccg gtggcttgca ccgtgtaata
     2761 gcccggtgct gcagctgctt cgctggcatg cacgtgcgcc tgagccgcca gcccaacggt
     2821 cagaacggac aacacccaag gtgcaaggga catggcaagg ggctccctgt gaaaatctgg
     2881 acattaatta agtaaactct aatgtaccat gttacgccac caatcgtgta ggtgcagcgc
     2941 gccagaagcg ctgtccaaac gcagccgtcg acgtagcaac tta
//
LOCUS       DAOMTQ010000252         2943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_9731_length_2943_cov_24.852839, whole genome shotgun sequence.
ACCESSION   DAOMTQ010000252 DAOMTQ010000000
VERSION     DAOMTQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9731_length_2943_cov_24.852839"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PK518_15380"
     CDS             <1..1545
                     /locus_tag="PK518_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM50339.1"
                     /translation="YQPDRQRRTQVLIAGAGVAGLAAARALRLAGVEDFALLELEDQA
                     GGNSRAGSVGGIACPLGAHYLPVPGDDAPEVQDLLEELGLRQRVAGRWQYGGDDGRHL
                     CHSPQERLFIDGQWQDGLLPVAGVGAATLAQYRRFAARVDALSRAARFTMPVVSGFRS
                     NKALAPVHQALDAITFAEWLAREGLDDAHLRWYLDYCCRDDYGAGSARVSAWAGIHYF
                     ASRHGFHAPGEGGADAEGVLTWPQGNGWLTQRLAAPLADRLHAGASVLRIAEGRHGVE
                     LDALDAASGQVVRWQARRCIVALPLFVAARLVQPVPDFLTEAARRLQWAPWLVANIHI
                     DRPLADRSGAAPAWDNVLYQDANPGGLGYVDAGHQRLDARAAQAAPTVLTYYQALGDL
                     PDGRSALLERPWTHWRDAILATLAGPHPDIHERAQRMELTRYGHAMAIPVPGLQVFLH
                     EISLSHLSGKRYQLSNGERAAVPPTPATPRLLFAHADWSGYSVFEEAFTRGHAAGVLA
                     AALAAR"
     gene            complement(1493..>2943)
                     /locus_tag="PK518_15385"
     CDS             complement(1493..>2943)
                     /locus_tag="PK518_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM50340.1"
                     /translation="APLRVLAVDAPPMARYDPASKSYSGIGVDVWCFITRELGLRHDL
                     LPGQNLSLADKLQQVQDGRADVFMPLSHQADRARLGLFTLPYYESYYAVIARKGRPLP
                     VHGLADLAPYRVGLVKGVALEPQVQAVVAAPRLTRFDQAGSEGMFQALRAGAIDLAVY
                     SRDIFEEKRYAHEFFDLEVIHTLRDDPRSYRFYFSPTPAHQRVAAAFDRYLAAIDSSA
                     SVAAHVIGERQFIERYVAQRSQRAMWQTTSVAGALLALVLGLGFLYYRRMAHRLAASH
                     QQVQQQQQALQAANQALEQQSLSDTLTGLPNRRAFDLALHREHARQQRTGQPLSLLLL
                     DVDHFKGVNDAYGHSIGDDYLRAVAQVLRKVVTRSTDLAARHGGEEFTCLLPDTSATQ
                     AQALAERIREATQRLTLPNARAWHPMLTLSIGVATLEAGPASAQQLLEQADGQLYVAK
                     RTGRNRVCATVLRGAVTEPPMPQPAPPPHARA"
BASE COUNT          387 a         1022 c         1088 g          446 t
ORIGIN      
        1 taccagcccg accgccagcg ccgcacccag gtgctgattg ccggcgccgg cgtggccggc
       61 ctggcggcgg cgcgcgcgct gcgccttgcc ggggtggagg acttcgcgct gctggagctc
      121 gaagaccagg ccggcggcaa cagccgcgcc ggcagcgtgg gcggcatcgc ctgcccgctg
      181 ggcgcgcatt acctgcccgt gcccggcgac gacgcgcccg aggtgcagga tttgttggag
      241 gagctgggcc tgcgccagcg cgtggccggg cgctggcagt acggcggcga cgacgggcgc
      301 cacctgtgcc acagcccgca ggagcggctg ttcatcgatg gccaatggca ggacggcctg
      361 ctgcccgtgg cgggggtggg cgcggccacg ctggcgcagt accggcgctt tgccgcgcgc
      421 gtggacgcgc tctcgcgcgc cgcgcggttc accatgccgg tggtcagtgg ttttaggtca
      481 aacaaggctc tggcgcccgt ccatcaagcg ctggacgcta tcactttcgc agaatggctg
      541 gcgcgcgagg ggctggatga cgcgcatctg cgctggtacc tggactactg ctgccgcgac
      601 gactacggcg ccggcagcgc gcgcgtatcc gcctgggccg gcatccacta ctttgccagc
      661 cgccatggct tccacgcccc gggcgagggc ggtgccgacg ccgagggcgt gctgacctgg
      721 ccccagggca atggctggct gacgcagcgc ctggccgcac cgctggccga tcgcctgcac
      781 gccggggcca gcgtgctgcg catcgccgag gggcgccatg gcgtggagct ggacgcgctg
      841 gacgccgcca gcggccaggt cgtgcgctgg caggcgcgcc gctgcatcgt ggcgctgccg
      901 ctgttcgtcg ccgcgcgcct ggtgcagccg gtgcccgact ttttgaccga ggccgcgcgc
      961 cgcctgcaat gggcgccctg gctggtggcc aatatccaca tcgaccgccc gctggccgac
     1021 cggagcggcg cggcgccggc ctgggacaac gtgctctacc aggacgccaa ccccggtggc
     1081 ctgggctatg tggacgccgg ccaccagcgc ctggacgcgc gcgccgcgca ggccgcgccc
     1141 acggtgctga cctactacca ggcgctgggc gacctgcccg acggtcgcag tgcgctgctg
     1201 gagcggccct ggacgcattg gcgggacgcc atcctggcga ccctggccgg gccgcatccg
     1261 gacatccatg agcgcgccca gcgcatggag ctcacgcgct atggccacgc catggccatc
     1321 ccggtgccgg gtctgcaggt ttttttacat gaaattagcc tctcgcactt atctggtaag
     1381 cgctaccagc tatcaaatgg ggagcgtgcc gccgtaccgc ccacgccagc cacgccgcgc
     1441 ctgctgttcg cccacgcgga ctggtccggc tactcggtgt tcgaggaggc tttcacgcgc
     1501 gggcatgcgg cgggggtgct ggctgcggca ttggcggctc ggtaaccgcg ccccgcagta
     1561 cggtggcgca gacgcggttg cgcccggtgc gcttggccac atagagctgg ccgtcggcct
     1621 gctccagcag ctgctgggcg ctggccgggc cggcttccag ggtggccacg ccaatgctca
     1681 gcgtcagcat ggggtgccag gcgcgggcgt tgggcagggt gagccgctgc gtcgcctcgc
     1741 gtatgcgttc ggccagggcc tgcgcctgtg tggcgctggt gtcgggcagc aggcaggtga
     1801 attcctcgcc cccgtggcgc gccgccaggt cggtggagcg ggtcaccacc ttgcgcagca
     1861 cctgggccac ggcgcgcagg tagtcgtccc cgatggagtg gccataggcg tcgttcacgc
     1921 ccttgaagtg atccacgtcc agcagcagca ggctcagcgg ctggcccgtg cgctgctggc
     1981 gtgcatgctc gcggtgcaag gccaggtcga aggcgcgccg gttcggcagg ccggtcaggg
     2041 tgtcggacag gctttgctgc tccagcgcct ggttggccgc ctggagggcc tgctgttgct
     2101 gctgcacctg ctggtggctg gcggccagcc ggtgcgccat gcgccggtag tacaaaaaac
     2161 ccaggccgag caccagcgcc agcagggcgc cggccacgct ggtggtctgc cacatggcgc
     2221 gctggctgcg ctgggccacg tagcgctcta tgaattggcg ctcgccgatc acatgcgcgg
     2281 cgacggatgc cgagctgtcg atggccgcca ggtagcggtc gaacgccgcc gccacgcgct
     2341 ggtgcgccgg cgtggggctg aagtagaagc ggtaggagcg cgggtcatcg cgcagggtgt
     2401 gtatgacctc caggtcgaag aactcgtgcg cgtagcgctt ttcctcaaag atgtcccggc
     2461 tgtagacggc caggtctatc gcgcccgcgc gcagcgcctg gaacatgccc tcgctgccgg
     2521 cctggtcgaa gcgggtgagc cggggtgcgg caaccacggc ctgcacctgt ggctccagcg
     2581 ccacgccctt gaccaggcca acgcggtagg gcgccagatc ggccaggccg tgcaccggca
     2641 gcggccgccc cttgcgggcg atgacggcgt agtaactctc gtaatacggc agggtgaaca
     2701 ggcccagccg ggcgcgatcg gcctggtggc tcagcggcat gaagacatcg gcgcgcccgt
     2761 cctgcacctg ctgcagcttg tcggccaggc tcaggttctg gccgggcagc aggtcgtggc
     2821 gcaggcccag ctccctggtg atgaagcacc acacatcgac gccgatgccg ctgtagctct
     2881 tgctggccgg gtcgtagcgc gccatcggcg gcgcatccac cgccagcacg cgcagcggcg
     2941 cca
//
LOCUS       DAOMTQ010000253         2880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_10063_length_2880_cov_29.631504, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000253 DAOMTQ010000000
VERSION     DAOMTQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10063_length_2880_cov_29.631504"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="motA"
                     /locus_tag="PK518_15390"
     CDS             <1..729
                     /gene="motA"
                     /locus_tag="PK518_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HRM50341.1"
                     /translation="GAFIINNQPKVLKATMGALPQLFKGGKYTKARYMELLAMLYEIL
                     QKARKEGLMAIEADVESPKDSEIFKKFPTVGSDHHVIEFTTDYLRMMVSGNLNAHEIE
                     SLMDSEIDTHHQEAHAPVAALTRLAGALPAFGIVAAVLGVVNTMGSVGQPPAVLGGMI
                     GSALVGTFLGILLAYGVVEPLAGLLEQKSEDAAKEFACIKSTLLASMQGYNPATAIEF
                     GRKVLFSGDRPSFSELENHVKGKK"
     gene            745..1683
                     /gene="motB"
                     /locus_tag="PK518_15395"
     CDS             745..1683
                     /gene="motB"
                     /locus_tag="PK518_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRM50342.1"
                     /translation="MAEKKLQPIIIKRIKKGGHAAHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTAKGELQGIASYFASPLKVAMQGGDGSGNSSSIIPGGGNDLSKVHGQVRRSES
                     DTNTNPRSIDTVRAERARVDAQRIKALQSKIDAMITGNPKLNEYRSQIRIDVTPDGLQ
                     IQIVDDQSRPMFDSGSALVKPYMRDILREIGAALGGVENRISLAGHTDAAPYGNGDRG
                     YSNWELSADRANASRRELVAAGMPDTKLGRVVGLAASDLLEPDSPRAAMNRRITITVL
                     THEAEERLLGKKTTVLAAPESRAEKPDNVGEPPKNQ"
     gene            1749..2135
                     /gene="cheY"
                     /locus_tag="PK518_15400"
     CDS             1749..2135
                     /gene="cheY"
                     /locus_tag="PK518_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HRM50343.1"
                     /translation="MSSALRFLIVDDFSTMRRIVRNLLKESGYTEADEAEDGVAALNK
                     LRNSHFDFVVSDINMPNMNGFQLLAEIKKDDKLKHLPVLMVTAEARKEDIVAAAQGGA
                     AGYIVKPFTKATLEEKVGLILKKMGL"
     gene            2138..2788
                     /locus_tag="PK518_15405"
     CDS             2138..2788
                     /locus_tag="PK518_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HRM50344.1"
                     /translation="MEVADQGLATGAGAGASTGTDDVHQKIGSLTRQLHTALNELGFA
                     DPLRGSMGELPDAQSRLSYIARLTGEAAEKVLGRVEQAKAQHDYIASETRRMVTSLVA
                     DPVAAVARGEIYNFLTDVERVTQEADGHLTEIMMAQDFHDLTGQVIARVVNLASTIEQ
                     QLVQLLLQTAPAGSAPAPTTPVRSEALQGPVVDAESRTDVVTDQSQVDDLLASLGF"
BASE COUNT          570 a          896 c          949 g          465 t
ORIGIN      
        1 ggggcgttca tcatcaacaa ccagcccaag gtgctcaagg ccaccatggg cgcgctgccg
       61 cagctgttca agggtggcaa gtacaccaag gcgcgctaca tggagctgct ggcaatgctc
      121 tatgaaatcc tgcagaaggc gcgcaaagag gggttgatgg ccatcgaggc ggacgtggag
      181 tcgcccaagg attcggagat cttcaagaaa tttcccaccg tgggctcgga tcaccatgtg
      241 attgagttca ccaccgacta cctgcgcatg atggtctcgg gcaacctgaa cgcacacgag
      301 atcgagtcgc tgatggacag cgagatcgac acccatcacc aggaggcgca tgcccccgtg
      361 gccgccctga cccgcctggc cggcgcgctg ccggccttcg gtatcgtggc cgccgtgctg
      421 ggtgtggtga acaccatggg ctcggtgggc cagccgccgg ccgtgctggg cggcatgatc
      481 ggatcggccc tggtgggtac ctttctgggc atcttgctgg cctatggcgt ggtggagccg
      541 ctggccggtc tgctggagca aaagagcgag gacgcggcaa aggagttcgc ctgcatcaag
      601 tccacgctgc tggccagcat gcagggctac aacccggcca cggcgataga gtttggccgc
      661 aaggtgctgt tctcgggcga ccggcccagc ttctcggagc tggagaacca cgtcaagggc
      721 aagaagtaag cggggtctgg cgccatggcc gagaagaagc tccagcccat catcatcaag
      781 cgcatcaaga agggcggcca tgccgcccat ggcggcgcct ggaagattgc gtacgccgac
      841 ttcgtgacgg ccatgatggc cttctttttg ctgatgtggc tgctgggctc cacggccaag
      901 ggggagctgc agggcattgc ctcgtacttc gcctcgccgc tcaaggtggc aatgcagggt
      961 ggcgatggct cgggcaacag ctccagcatc attcctggcg gcggcaacga tttgtccaag
     1021 gtgcatggcc aggtgcgccg ctcggagtcg gacaccaaca ccaacccgcg cagcatagac
     1081 acggtgcgcg ccgagcgcgc gcgcgtggat gcgcagcgca tcaaggcgct gcagtccaag
     1141 atcgacgcca tgattacggg caaccccaag ctcaacgaat accgctcgca gatacgcatc
     1201 gacgtgacgc ccgacgggct gcagatccag atcgtggacg accagagccg gcccatgttc
     1261 gacagcggca gcgcgctggt caaaccttat atgcgcgaca tactgcgcga gatcggtgcg
     1321 gccttagggg gcgtggagaa ccgcatcagc ctggccggcc ataccgacgc cgcgccctat
     1381 ggcaatggcg accggggcta cagcaactgg gagctgtcgg ccgaccgggc gaatgcctcg
     1441 cgccgcgagc tggtggccgc cggcatgccc gacaccaagc tgggccgcgt ggtcggcctg
     1501 gcggccagcg acctgctgga gccggacagc ccgcgcgcgg cgatgaaccg gcgcatcacc
     1561 atcaccgtac tcacgcacga ggccgaggag cggctgctgg gcaagaaaac cacggtattg
     1621 gcggcgcctg aaagccgtgc cgaaaagcct gacaatgtcg gcgaaccccc caaaaatcag
     1681 taactatttg ttcatgactg atttttttaa cgacaatcac ctctccactt gcaccgaaag
     1741 ggtcctttgt gtcctctgcc cttcgctttc tgatcgttga tgacttttcc accatgcgcc
     1801 gcatcgtgcg caacctgctc aaggagagcg gctataccga agccgacgag gccgaggacg
     1861 gcgtggccgc gctgaacaag ctgcgcaaca gccatttcga ctttgtggtc tcggacatca
     1921 acatgccgaa catgaacggc tttcagctgc tggccgagat caagaaggac gacaagctca
     1981 agcacctgcc ggtgctgatg gtcacggccg aggcgcgcaa ggaggacatc gtcgccgctg
     2041 cccagggtgg cgcggccggc tacattgtga aacctttcac caaggccacg ctggaagaaa
     2101 aagtgggcct gatcctcaag aaaatggggt tgtgaccatg gaggtggcgg atcaaggcct
     2161 ggcaacaggc gcaggcgcag gcgcaagcac tggcacggac gatgtgcatc agaagatcgg
     2221 ctcgctcacg cgccagctgc acaccgcact caacgagctg ggctttgccg acccgctgcg
     2281 cggcagcatg ggcgagctgc ccgatgcgca aagccgcctg tcctacatcg cccgcctgac
     2341 cggcgaggcg gccgagaagg tgctgggccg cgtggaacag gccaaggccc agcacgacta
     2401 catcgccagc gaaacacggc gcatggtgac atcgctggtg gccgacccgg tggccgccgt
     2461 ggcccggggc gagatctaca actttctgac cgacgtggag cgcgtgaccc aggaggccga
     2521 cggccacctg accgaaatca tgatggccca ggactttcat gacctgaccg gccaggtgat
     2581 tgcgcgcgtg gtcaacctgg ccagcaccat cgagcagcag ctggtgcagc tgctgctgca
     2641 gacagcaccc gcgggcagcg ccccggcgcc gactaccccc gtacgcagcg aagccctgca
     2701 ggggccggtg gtggacgccg agtccagaac cgacgtggtg acggatcagt cgcaggtgga
     2761 cgacttgctg gccagcctgg ggttttgatc gctggcttgg ctggttcgcc aggagtcgca
     2821 ggcggccata ttttggccat ctgccggtgt aggcgcagtg tggaggcgct tgcggctagt
//
LOCUS       DAOMTQ010000254         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_10093_length_2874_cov_22.000000, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000254 DAOMTQ010000000
VERSION     DAOMTQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10093_length_2874_cov_22.000000"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /gene="cbiB"
                     /locus_tag="PK518_15410"
     CDS             <1..894
                     /gene="cbiB"
                     /locus_tag="PK518_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HRM50345.1"
                     /translation="WGEPPAPLHPVVWMGRALGACGARLAPARATGRDLWSFWLAALI
                     WCALAAIVLFLAAALQWLAWAYLPAWGVALVLGLLLKPLLAWRMLRDEVLAVESALGQ
                     SLAAGRARLAWLVSRDVQQLDEAAVRESAIETLAENLCDSVVAPLFWFVLLGLPGAAL
                     YRFANTADAMWGYPGMRGGRYWQWAGKWAARADDVLSWLPARLTALLLWAAALGAVRW
                     RDVRTQARVTPSPNGGWPMGAMALALGVRLSKPGVYVLNAAGRVVLAGDAERALRLAA
                     RAIAALVMLAVVGLESITHFK"
     gene            1027..1305
                     /locus_tag="PK518_15415"
     CDS             1027..1305
                     /locus_tag="PK518_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM50346.1"
                     /translation="MEATKVGIREFRADLAEYIAANSPVAVTRHGQTVGYFIPVHGQA
                     EADLVALQKASQSLDKLLSAQGVDVEDIVTDFKKARKKSTSGAGAGAK"
     gene            1302..>1639
                     /locus_tag="PK518_15420"
     CDS             1302..>1639
                     /locus_tag="PK518_15420"
                     /inference="COORDINATES: protein motif:HMM:NF021634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRM50347.1"
                     /translation="MNDAAIVLDANILIRAVLGQRVRELIRAHASTVKFFAPDVAYAD
                     ARKYLPALLDKRGVDPVPAMQVLDALERIVHALDVEVYGGLQQQALRRIKTRDADDWP
                     VLACAMVLGCP"
     assembly_gap    1640..1739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1763..2761
                     /locus_tag="PK518_15425"
     CDS             1763..2761
                     /locus_tag="PK518_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM50348.1"
                     /translation="MSDQAPAHGGPDALGVPLHDFSTNANACGPCPVAFAALQQADRQ
                     HYPDPAYTRLRALLAGFHGVDVERIVIAASASEFIHRITAHAARSGLRRAVSPAHGYG
                     DYARAARNWGLDCGTGQAMALALHWACEPASPLGMADAALAAWVQRQGDGALRVLDCA
                     YAPLRLDGQATALPEGVWQLWSPNKALGLTGVRAAYAIAPAAAVTDGSAAALRALAPS
                     WPLGVDGVALLSAWVQADTQDWLAQSLATLRQWKAAQLRLCADLGWAVLPGSLANFFT
                     ARPPVPDLAAALQALRAQGIKLRDCASFGLAGHVRLGVLAPAAQRALAAAWRQWAP"
BASE COUNT          365 a          871 c         1032 g          506 t
ORIGIN      
        1 tggggcgagc cccccgcgcc cttgcacccc gtggtgtgga tggggcgcgc gctgggcgcc
       61 tgcggcgcgc gtctggcgcc ggcccgggct acggggcgcg atttatggtc tttttggctt
      121 gcagcgctta tttggtgcgc gctggcagct atcgttttgt ttttggctgc agccctgcag
      181 tggctggcct gggcctatct gccggcctgg ggcgtggcgc tggtgctggg gctgctgctc
      241 aagcccctgc tcgcctggcg catgttgcgt gacgaggtgc tggcagtgga gtccgccctg
      301 gggcagtcgc tggccgccgg gcgtgcgcgc ctggcctggc tggtcagccg cgatgtgcaa
      361 cagttggacg aggcggcggt gcgcgagagc gccatcgaga ccctggccga gaacctctgc
      421 gactcggtgg tcgcgccgct gttctggttt gtgctgctgg gcctgccggg cgcggcgctg
      481 taccgctttg ccaatacggc cgatgccatg tggggctacc ccggcatgcg tggcgggcgc
      541 tactggcagt gggctggcaa atgggctgcg cgggccgacg atgtgctgtc gtggctgccg
      601 gcgcggctga cggcgttgct gctgtgggcg gcggcgctgg gcgcggtgcg ctggcgcgat
      661 gtgcggacgc aggcgcgcgt gacgccgtcg cccaatggcg gctggcccat gggcgcgatg
      721 gccctggcgc tgggggtgcg gctgtccaag ccgggggtgt atgtgctgaa tgccgcgggg
      781 cgggtggtgc tggcggggga tgcggagcgg gcgttgcgct tggcggccag ggctattgcg
      841 gcactggtga tgttggcggt ggtgggcttg gagtcaatca cccacttcaa atagcggaat
      901 cacgcagcgc actattggcg tgtcagggtc aggtgtgatt gcgctgtgcg ttatcggcgt
      961 aatagccagc gaaggtcgta ttgcttaaaa tgtgtacaca ttgatccaca agtcaggagt
     1021 tactccatgg aagccacaaa ggtcggaatt cgtgaatttc gtgcggatct ggccgagtac
     1081 attgctgcga acagcccggt cgcggtgact cgccacggtc agacggtcgg ttacttcatt
     1141 cctgtacacg gccaggccga ggcggatttg gtggcgctcc agaaagccag ccagtcactc
     1201 gacaagctgc tgtctgcgca aggcgtggac gtagaggaca tcgtgaccga cttcaagaag
     1261 gcgcgaaaga agagcacttc cggcgccggt gccggggcca aatgaacgac gcggccattg
     1321 ttctggatgc gaacatcctg atccgcgcgg tgcttggcca gcgtgtccgt gaactgatcc
     1381 gggcgcatgc ctccacggtc aagttcttcg cgcccgatgt ggcgtatgcg gatgcgcgca
     1441 aatacctgcc ggcgctgctg gataaacgtg gcgtcgatcc tgtccccgcc atgcaagtgc
     1501 ttgatgcact cgagcgcatt gttcatgccc tggacgtgga ggtctatggc ggattgcaac
     1561 agcaggcact gcggcgcatc aagacacggg atgccgacga ttggccggtg ttggcttgcg
     1621 ccatggtttt ggggtgcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1741 cgcttggcta tagtgcccgc ccatgtctga ccaagccccg gcgcacggtg gccccgacgc
     1801 cctgggcgtg ccgttacacg acttttccac caatgccaat gcctgcgggc cttgccccgt
     1861 ggcctttgcc gcgctgcagc aagccgaccg ccagcattac cccgatccgg cctatacccg
     1921 gctgcgcgcg ttgctggcgg gctttcatgg ggtggacgtc gagcgcatcg tgatcgccgc
     1981 cagtgccagc gagttcatcc atcgcatcac ggcccatgcg gcgcgcagcg gcctgcgccg
     2041 cgccgtatcg ccggcacatg gctatggcga ctatgcgcgc gcggcgcgca actggggttt
     2101 ggattgcggc accgggcagg ccatggcgct cgcgctgcac tgggcctgcg agcccgcgag
     2161 cccgctgggc atggcggatg cggcgctggc cgcatgggtg caacggcagg gcgatggcgc
     2221 gctgcgcgtg ctcgactgcg cctatgcccc gctgcgcctg gacggtcagg ccacggcgct
     2281 gcccgaaggg gtttggcagc tgtggtcgcc caacaaggcc ctgggcctga cgggcgtgcg
     2341 cgcggcctat gccattgcgc cggccgctgc cgtgaccgat ggcagcgctg cggcgctacg
     2401 ggcgctggcg ccgtcctggc cgctgggggt ggatggcgtg gcgctgctca gcgcctgggt
     2461 gcaggccgac acgcaggatt ggctggccca gtcgctcgcc accttgcgcc agtggaaggc
     2521 ggcgcagctg cggttgtgcg ccgatctggg ctgggccgtg ctgccgggca gcctggccaa
     2581 cttcttcacc gcccgcccgc cggtgccaga cctggccgcc gcgttgcagg ccctgcgcgc
     2641 gcagggcatc aagctgcgcg actgcgcctc gttcggcctg gccggccatg tgcgcctggg
     2701 cgtgctggcg ccggctgcgc agcgggccct ggccgcagca tggcgacaat gggcgccatg
     2761 acgcacaaga cattgcccgg cccggcgcgg gccatcatgg tgctgggcac cagcagcggc
     2821 gccggcaaga gctggctggc cacggccctg tgccgccact acagcaacca gggg
//
LOCUS       DAOMTQ010000255         2872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_10100_length_2872_cov_25.291800, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000255 DAOMTQ010000000
VERSION     DAOMTQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10100_length_2872_cov_25.291800"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1567)
                     /gene="egtB"
                     /locus_tag="PK518_15430"
     CDS             complement(284..1567)
                     /gene="egtB"
                     /locus_tag="PK518_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HRM50349.1"
                     /translation="MNQSTDTTTAQARNDDAVGRYLTVRAATEALARPLSAEDCCVQS
                     MPDASPAKWHLAHTSWFFETFILERHEQGFCAFDAAFRVLFNSYYQGVGRQHPRPQRG
                     LLTRPSLATVLAYRADVDARMLRLLQQDGLPEPIWSLLELGLQHEQQHQELLLTDIKH
                     LLWCNPLWPAYRDAPAVADSEAAAPSDWQPFEGGLVQTGHAGAGFAFDNELPRHSVFL
                     QPYALATRPVMNAEYLAFMADGGYANPAHWLAEGWDWCRSQQITHPLYWRQEEAGWQE
                     FTLAGGRPLVPRQPVVHLSYYEADAYARWAGARLPTEAEWEAAAGQADSQGHFADSGL
                     FQPLPERQQGAGLRQMFGDVWEWTQSSYAPYPGFATAPGAVGEYNGKFMINQYVLRGG
                     SCATPAGHVRASYRNFFPAAARWQFTGVRLARDMR"
     gene            complement(1582..2592)
                     /gene="egtD"
                     /locus_tag="PK518_15435"
     CDS             complement(1582..2592)
                     /gene="egtD"
                     /locus_tag="PK518_15435"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HRM50350.1"
                     /translation="MPVIAQSQSKSQRIAHILHGPEPRRGLPAAGALLDRRQEITRAL
                     LAPRASISPKYFYDQRGSELFEAITRLPEYYPTRTEQALLQQHGQAIAAAVGSGGVVI
                     EPGAGNCAKGLALCRSLAATHFVGVDISLAYLQGAVARLRQALPGLDARAVGGDITAG
                     VRLPADIPRQRRLVFYPGSSIGNFDPPQAVALLAQMRALALDGGGLLIGIDLPKDRAV
                     LEAAYDDAAGVTAAFNRNVLAHLNRLIGSDFAPQCWRHRAFFNAAASRIEMHLEACGA
                     QRVRWPGGGRDFAAGERIHTENSYKHALPAFTALLRQAGFAGTQHWTDERGWFAMVYA
                     RA"
BASE COUNT          488 a          985 c          950 g          449 t
ORIGIN      
        1 aagcgttgtc gatgttgcct gaaatgctat aaaaaagatg tgtgcataca caaccccaga
       61 gggagagggg agtaaaaccg gcgcaaccgg ccgccctcgc ccccttgggg ctgaaggctg
      121 cggctccagt gccgcctgcg gggaactgga cagcctgcag agcacagcgt ctcgctgccg
      181 cccccgggag gggtgagggg catctgccac atgtacatag tgaggcattt ttagcctccg
      241 gcgcttatac agcaagcgct ggaagctatg cttttgaaag cattcagcgc atatcgcgcg
      301 ccagtcgcac gcccgtgaac tgccagcgcg ccgcggccgg aaagaagttg cggtagctgg
      361 cgcgcacatg gcccgccggc gtggcacagg agccgccgcg cagcacatac tggttgatca
      421 tgaacttccc gttgtactca cccaccgcgc ccggcgctgt ggcaaagccg gggtatggcg
      481 cgtagctgga ctgggtccat tcccagacat cgccaaacat ctgccgcagc cctgccccct
      541 gctggcgttc cggcaggggt tggaacaacc cgctgtcggc gaaatgcccc tgggaatccg
      601 cctggcctgc ggccgcttcc cattcggcct cggtgggcag gcgcgcgccg gcccagcggg
      661 cataggcatc ggcctcgtaa tacgacagat gcaccaccgg ctggcgcggc accagcggcc
      721 ggccgcccgc aagcgtgaat tcctgccagc cagcctcctc ctggcgccag tacagcgggt
      781 gggtgatctg ctggctgcgg caccagtccc agccctcggc cagccagtgc gcggggttgg
      841 cataaccacc atcggccatg aaggccaggt actcggcatt catcaccggc cgcgttgcca
      901 gcgcataggg ctgcagaaag acgctgtggc gcggcagttc gttgtcgaag gcaaagcccg
      961 cgcctgcatg gcctgtctgc accaggccgc cctcgaacgg ctgccagtcg ctgggcgcag
     1021 ccgcctcgct gtctgccacc gccggggcat ctcggtaggc gggccacaac gggttgcacc
     1081 acagcaaatg cttgatgtcg gtcaacagca gctcctggtg ctgctgctcg tgctgcagcc
     1141 ccagctccaa caaactccag atgggctccg gcaagccatc ttgctgcagc aggcggagca
     1201 tgcgcgcatc gacgtcggcg cggtaggcca gcacggtggc cagcgacggc cgcgtcagca
     1261 ggccgcgctg cgggcgcggg tgctggcgcc ccacgccctg gtagtaggaa ttgaacagca
     1321 cgcgaaacgc cgcgtcgaac gcgcaaaagc cctgctcgtg ccgctccagg atgaaggttt
     1381 cgaagaacca gctggtatgt gccagatgcc atttggcggg gctggcgtcg ggcatggact
     1441 gcacgcagca gtcctcggcc gacagcggcc gcgccagcgc ctcggtggcg gcgcgcacgg
     1501 tcaggtagcg gcccaccgca tcgtcgttgc gcgcctgcgc tgtggtggtg tcggtgctct
     1561 ggttcatggt ggggcgcatc ctcacgcccg ggcgtacacc atggcaaacc agccgcgctc
     1621 gtccgtccaa tgttgggtgc cggcaaaacc cgcctggcgc agcagcgcgg taaaggccgg
     1681 cagcgcatgc ttgtagctgt tctcggtatg gatgcgctcg ccggcggcaa agtcgcgccc
     1741 gccgccaggc caacgcacgc gctgcgcgcc gcaggcctcc aggtgcatct ctatgcgcga
     1801 cgcagcggcg ttgaagaacg cccggtggcg ccagcactgc ggggcaaagt cgctgcctat
     1861 caggcggttc agatgtgcca gcacattgcg gttgaaggcc gctgtcacgc ccgccgcatc
     1921 gtcataggcg gcctccagca cggccctgtc cttgggcagg tctatgccga tcagcaggcc
     1981 acccccgtca agggccagcg cacgcatctg cgccagcagc gccacggcct gcggcggatc
     2041 gaagttgcct atggatgaac ccgggtagaa caccaggcgc cgctggcgcg gaatatccgc
     2101 cggcagccgc acgcccgccg tgatgtcgcc gcccacggcg cgcgcatcca agcccggcag
     2161 cgcctggcgc aggcgcgcca ccgcgccctg caggtaggcc agcgaaatat ccacgcccac
     2221 gaaatgcgtg gcggccaggc tgcggcacag cgccaatccc ttggcgcagt tgccggcgcc
     2281 gggctctatc accacgccgc cgctgccaac ggcagccgca atggcctggc catgctgctg
     2341 caacagagcc tgttcggtgc gcgtggggta gtactcgggc aggcgcgtga tggcctcgaa
     2401 cagctcggag ccgcgctggt catagaaata cttgggcgag atgctcgccc ttggcgccag
     2461 caaggcgcgt gtgatctcct gccggcgatc caggagcgcc cccgccgccg gcagcccccg
     2521 ccgtggttca gggccgtgca ggatgtgcgc gatgcgctgg gacttggact gggactgggc
     2581 aatgacgggc ataacgccgc agcgtagtga acccggcacc gcccaccagc cagccatggc
     2641 ccgcaccgcg ggtaggacga gcgcgcaaat cccgcgccaa ggcttgcccg atcgcgccgg
     2701 ccatggcagg cagcggttta gccgcctatg gctttttttg gccgtagccc tttaccattg
     2761 ggcgctgtca gctatgtttt ttgataaaca tgaggctgcg gcaatacccc tcacaaggcc
     2821 ttcttcaact ccgccacctc ggcctggccg ggaaacttgt ccccggcggc ag
//
LOCUS       DAOMTQ010000256         2812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_10440_length_2812_cov_23.418571, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000256 DAOMTQ010000000
VERSION     DAOMTQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10440_length_2812_cov_23.418571"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PK518_15440"
     CDS             <1..876
                     /locus_tag="PK518_15440"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HRM50351.1"
                     /translation="ELIKFADQAMYTAKAGGRNRFAHFNPAMQAQIVRRAMLLRDLRQ
                     ALQGRQMLLHYQPIIDLRSGQVAKAEALLRWQHPQHGLVSPMEFIPLAEESGLIHEIG
                     EWVLRQAVQTVAHIRAHQARLLPINVNVSAKQLARPGFSATHWARLMDAHGLPRKAIT
                     MEITEGTLVESSQLVRDCLTGFVQHGVEVALDDFGTGFSSLAYLKRFDIDYLKIDASF
                     VRGLEAGGNDHAIVEAIITMAHKLGIQTIAEGVETTAQRDLLKALGCDHAQGYLFAKP
                     LPLAAFEAYISTNPA"
     gene            complement(1137..1481)
                     /locus_tag="PK518_15445"
     CDS             complement(1137..1481)
                     /locus_tag="PK518_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRM50352.1"
                     /translation="MQIQVHTNDNIQGGESLIRWAQDEAAAKLARFREHVTRVELFLS
                     DVDAGKSGVDDKRCVIEARPAGRQPVAATAEAAKVADAVAAALDKLVRSLGDDITRAK
                     DRQGRESIRTAE"
     gene            1684..2565
                     /locus_tag="PK518_15450"
     CDS             1684..2565
                     /locus_tag="PK518_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRM50353.1"
                     /translation="MSLLSHSASRLKIGLSACFSHADPDRSLFTNKTLQYVEQSIAHW
                     IMSAGALVVMVPCPTGETARGDVTLKHYAEWLDGVVMHGGSDVWPGSYGEEPLKDAWL
                     GDRVRDLYDLAVVEAFSQAGKPIFGVCRGLQLINVAFGGTLYQDIETQHPGALQHRNA
                     ITYDQHVHEVDIVPHTQLARLYPGRRRVTVNSIHHQGIKRLAPGFDIEAWSHPDGLPE
                     AIRRQGGNGRGYIAATQWHPEFRSREGVTLDDMPLLRDFLAACSQARARPRPGRGPLQ
                     IRDRAERLLRRALLRRH"
     gene            complement(2627..>2812)
                     /locus_tag="PK518_15455"
     CDS             complement(2627..>2812)
                     /locus_tag="PK518_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM50354.1"
                     /translation="ADGQPQVFNVVKIKGPEVHLDGNHPLAGRALRFACKVTEVRAAS
                     AEEIAHRHVHGGHGHHH"
BASE COUNT          481 a          957 c          897 g          477 t
ORIGIN      
        1 gagctgatca agttcgccga ccaggccatg tacaccgcca aggccggggg gcgtaaccgt
       61 ttcgcccact tcaacccggc catgcaggcc cagatcgtgc gccgcgccat gctgctgcgc
      121 gatctgcgcc aggcgctgca ggggcggcaa atgctgctgc actaccagcc catcatcgac
      181 ctgcgcagcg gccaggtggc caaggccgag gcgctgctgc gctggcagca tccgcaacac
      241 ggcctggtca gccccatgga gttcataccg ctggccgagg agtcgggcct gatccatgag
      301 atcggcgaat gggtgctgcg ccaggcggtg caaaccgtgg cgcatatccg tgcgcaccag
      361 gcgcggctgc tgcccatcaa cgtcaacgta tcagcgaaac agctggcccg ccccggcttc
      421 agcgccaccc actgggcccg cctgatggat gcccacggcc tgccgcgcaa ggccatcacc
      481 atggagatca ccgagggcac gctggtggag agctcgcagc tggtgcgtga ctgcctcacg
      541 ggctttgtcc agcacggggt ggaggtggcg ctggacgact ttggcaccgg cttttcatcg
      601 ctggcctacc tgaagcgctt tgacatcgac tacctgaaga tcgacgcctc cttcgtgcgc
      661 gggctggagg ccggcggcaa cgaccacgcc atcgtcgagg ccatcatcac catggcgcac
      721 aagctgggca tacagaccat cgccgagggg gtggaaacca cggcccagcg cgacctgctc
      781 aaggccctgg gctgcgacca tgcgcagggc tatctgttcg ccaaaccgct tccgttggcg
      841 gcattcgagg catacatcag caccaacccg gcatagctcg cggcaggcct attgacccgg
      901 caacaaaggg ttgaccgtca tcccggcgaa agccgggatc cagggacgcc cttagccagc
      961 gtagctgctg gatcccggct ttcgccggga tgacgacagt ccacaaccgt tagttgccgg
     1021 ttctataaga acgcaaaggg ctgcgaacgc attgcatgcg cgcagccctt tcttgaacaa
     1081 aaaaatgctc taacgctctt tcagcaaacg ccggcagcta ttattttgat agaagatcac
     1141 tccgccgtgc gaatgctttc gcggccctgg cgatccttgg cgcgggtgat gtcgtcgccc
     1201 agcgagcgca ccagcttgtc cagtgccgcg gccacggcgt cggccacctt ggcggcctcg
     1261 gcggtggctg ccaccggctg gcggccggcg gggcgggcct cgatcacgca gcgcttgtcg
     1321 tccacgccgg acttgcccgc gtccacgtcg gacaggaaca gctccacgcg cgtcacatgt
     1381 tcgcgaaagc gcgccagctt ggctgccgcc tcgtcctggg cccaacggat cagcgactca
     1441 ccgccctgga tgttgtcgtt ggtgtgcact tggatctgca tgtctatcct ccttggttga
     1501 aaccaggccc gaacccgggc ccttggtatc catcatgcct gagccggcaa gctggcgctt
     1561 gaatcccatc cacaggcggg tggcggcatg gacagtggcc cgggcgccac attccccctg
     1621 ctgttggcag taccgcagcc tgcgcctaca gacgtttccg gatgtcaatg ctacaactgt
     1681 tgcatgtcgc tcctgtccca ttccgccagc cgcttgaaga ttggcctgtc ggcctgcttc
     1741 tcgcatgccg accccgatcg ctcgctgttc accaacaaga ccctgcagta cgtggagcag
     1801 tcgattgcgc actggatcat gtcggccggc gcactggtgg taatggtgcc ctgccccacg
     1861 ggcgagacgg cgcgcggcga tgtcacgctc aagcattacg ccgaatggct cgatggcgtg
     1921 gtcatgcacg gcggctcgga tgtctggccc ggcagctatg gcgaggagcc gctcaaggac
     1981 gcctggctgg gtgaccgcgt gcgcgacctg tatgacctgg cggtggtcga ggccttcagc
     2041 caggccggca agcccatttt tggcgtgtgc cgcgggctgc agctgatcaa cgtggccttt
     2101 ggcggcacgc tgtaccagga cattgagacc cagcaccccg gcgccctgca gcaccgcaac
     2161 gccatcacct acgaccagca tgtgcacgag gtggacatcg tgccccacac gcagctggcg
     2221 cggctgtacc cggggcgccg gcgcgtcacc gtcaacagca tccaccacca gggtatcaag
     2281 cggctggcgc cgggttttga catcgaggcc tggagccacc ccgacggcct gcccgaggcc
     2341 atacgccgcc agggcggcaa cggacgcggc tacatcgcgg ccacgcagtg gcacccggag
     2401 ttccgctcac gcgagggcgt gacgctggac gacatgccgc tgctgcgcga ctttctggcc
     2461 gcctgcagcc aggcccgcgc gcgtccgcgg cccggccgcg gcccgttgca gatccgcgac
     2521 cgcgccgagc gcctgctgcg ccgcgcgctg ctgcggcgcc attgatgcgc aatgaaatac
     2581 ggcgctaacg cttatctgta aagcgccagc agctatcaat aaatgatcag tggtgatggc
     2641 catggccgcc atgcacatgg cggtgggcaa tctcctcggc gctggcggcg cgcacctcgg
     2701 tgaccttgca ggcaaagcgc agggcgcggc cggccagcgg gtgattgccg tccaggtgca
     2761 cctcggggcc cttgatcttg acgacattga agacctgcgg ttggccgtcg gc
//
LOCUS       DAOMTQ010000257         2752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_10788_length_2752_cov_23.993326, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000257 DAOMTQ010000000
VERSION     DAOMTQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_2752_cov_23.993326"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /gene="trbE"
                     /locus_tag="PK518_15460"
     CDS             complement(<1..2285)
                     /gene="trbE"
                     /locus_tag="PK518_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005304525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HRM50355.1"
                     /translation="MLNLAEYRQRPALLADWLPWAGLVAPGVVLNKDGSFQRTARFRG
                     PDLDSATQGELIATSARLNNALRRLGSGWALFIEAERRSAADYPHSDFPEPLSWLVDE
                     ERRAAFEDSGNHFESGYHLTLAYLPSEESRARAAGLLYENRPTEGVDWRERLHAFVAE
                     TDRIFDLLDGVMPEIAWLDDSQTLTYLHATISTRRYRVGVPEVPFHIDALLTDSALIG
                     GLAPMLGEQHLRVVSVRGFPTSTWPGILDDLNRLGFAYRWSTRFLCLDKAEAEKELSR
                     LRRQWFAKRKNVIALLRETIFQQESPLVDTDANNKAADADAALQELGSDQVAFGYLTA
                     TVTVMDADAAVADEKLRMVERVIQGRGFVTIPETLNAVDAWLSSIPGNAYANVRQPIV
                     STLNLAHMMPVSAVWAGQERNAHLDGPPLIVTRTDGATPFRLVTHIGDVGNTLVVGPI
                     GMGKSVLLATMAMQFRRYRGSRIFAFDMGRSIRAAILGMGGEHYDLGADDEIAFQPLA
                     RIDREGYRTWASEWVDGRLLHEGAAVGPDEKAAIWSALGSLASAPVEQRTLTGLTALL
                     QSNTLRQALAPYVLGGAHGRLLDADHDRLGTADVQAFEMEELMHSKAAVLAVLRYLFA
                     RFEERLDGAPTLLILDEAWLFLDDPVFAARIRQWLKTLRKKNVSVIFATQSLADVKDS
                     AIAPAIIESCASRIFLPNPQATEPQIRTIYEGFGLNSRQIEIVATAQPKRDYYYQSRL
                     GNRVFDLDLGPATLAFAGASTP"
     gene            complement(2296..2568)
                     /locus_tag="PK518_15465"
     CDS             complement(2296..2568)
                     /locus_tag="PK518_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HRM50356.1"
                     /translation="MSGPDTFAAGFEVPLHRSLTEPILLGGAPRTVAIANGTLAAAVG
                     LGLQLWIPGVVLWIVGHSLAMWGARVDPQFMQVFARHIKHRPLLDV"
     gene            complement(2565..>2752)
                     /locus_tag="PK518_15470"
     CDS             complement(2565..>2752)
                     /locus_tag="PK518_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HRM50357.1"
                     /translation="QGPVARIVAVIIIIATGLALAFGDTSGGFRKLIQIVFGLSIAFA
                     ASSFFLSFFSFSGGAVV"
BASE COUNT          484 a          903 c          934 g          431 t
ORIGIN      
        1 ggcgtggaag cgcccgcgaa ggccagcgtc gccggcccca ggtcgaggtc gaacacgcgg
       61 ttaccgaggc gggattggta gtagtagtcg cgcttgggtt gggcggtggc gacgatctcg
      121 atctggcgcg agttcagccc gaagccttcg tagatcgtgc gaatctgcgg ttcggtcgcc
      181 tgcggattcg gaaggaagat gcggcttgcg cagctttcga tgatggccgg cgcgatggct
      241 gaatccttca cgtctgcgag cgactgcgtg gcgaagatga cgctgacgtt cttcttgcgc
      301 agcgtcttga gccactggcg gatgcgcgcc gcaaacaccg ggtcatcgag gaacagccac
      361 gcttcatcaa ggatcagcag cgtgggcgca ccatccagcc gctcctcgaa gcgcgcgaac
      421 aggtagcgca gcaccgcgag cacagcggcc ttgctgtgca tcaattcctc catctcgaac
      481 gcctgcacgt cggccgtgcc gaggcggtca tggtcggcat ccagcagcct gccgtgcgcg
      541 ccgcccagga cgtagggcgc gagtgcctgg cgcaacgtgt tcgattgcag caaggccgtc
      601 aggcccgtga gcgtgcgctg ctccactggc gcgctggcca ggctccccag cgcagaccag
      661 atggccgcct tctcgtccgg gccgacggcc gcgccttcgt gcagcaggcg gccgtccacc
      721 cattcgctgg cccaggtgcg gtagccctcg cggtcgatgc gtgcgagcgg ctggaaggcg
      781 atctcgtcat ccgcgcccag gtcgtagtgc tcgccgccca tgcccaggat ggccgcgcgg
      841 atcgagcgcc ccatgtcgaa cgcgaagatg cgcgagccgc gatagcggcg gaactgcatc
      901 gccatcgtcg ccagcagcac cgacttgccc atgccgatgg ggccaaccac cagcgtgttg
      961 cccacgtcgc cgatgtgcgt caccagccgg aacggcgtgg cgccgtctgt gcgcgtgacg
     1021 atcagcggcg ggccatcgag atgcgcattg cgttcttgac cggcccatac cgccgacacc
     1081 ggcatcatgt gcgccaggtt cagcgtcgag acgatgggct ggcggacatt ggcgtatgcg
     1141 ttgcctggaa tggacgaaag ccacgcatcc acggcgttga gcgtttcagg gatggtgacg
     1201 aagccgcgcc cctgaatgac gcgctccacc atgcgcagct tctcgtcggc tacggcggcg
     1261 tccgcgtcca tgacggtgac ggtcgccgtc aggtagccga aggcgacttg atcgctgccc
     1321 agctcctgca aggcagcgtc ggcatctgcg gccttgttgt tggcgtcggt atcgaccagc
     1381 gggctttcct gctggaagat cgtctcgcgc agcagcgcga tgacgttctt ccttttcgca
     1441 aaccactggc ggcgcaggcg ggaaagctcc ttctccgcct cggctttgtc gaggcacaga
     1501 aatcgcgtac tccagcgata cgcaaagccc aggcgattga ggtcgtccag aatccccggc
     1561 caggtcgagg tcgggaaacc gcgcaccgac accacgcgca gatgctgctc gcccagcatc
     1621 ggcgccagcc cgccgatcag cgcggaatcg gtcagcagcg cgtcgatgtg gaacggcact
     1681 tcgggcacac cgacgcggta acgtcgcgtg gagatggtcg cgtgcaggta ggtcagcgtc
     1741 tggctgtcat cgagccacgc gatttccggc atcacgccat cgagcaggtc aaagatgcga
     1801 tccgtttcag cgacgaaggc gtgcagccgt tcgcgccaat ccacgccttc ggtcggccgg
     1861 ttttcgtaca gcagacccgc ggcgcgggca cgggattcct ccgatggcag gtaggccagc
     1921 gtcaggtgat agccgctctc gaagtggttg cccgagtcct cgaaggcagc gcggcgttct
     1981 tcatcgacca gccaggacag cggttcgggg aaatcggagt gcgggtagtc ggcggcagac
     2041 cggcgctcgg cttcgatgaa cagcgcccag cccgaaccca ggcggcgcag cgcgttgttc
     2101 aagcgcgccg atgtggcgat cagctcgcct tgcgtcgcgc tgtcgaggtc ggggccacga
     2161 aagcgcgccg tgcgctggaa cgagccatcc ttgttcagca cgacgcccgg cgcgaccagc
     2221 ccggcccacg gcagccagtc ggcaagcaag gccgggcgct ggcggtattc ggcaaggttc
     2281 agcatggcat tcccctcaca cgtccagcag cgggcggtgc ttgatgtgtc gcgcgaagac
     2341 ctgcatgaac tgcggatcga cgcgcgcacc ccacatcgcc agcgaatggc cgacgatcca
     2401 gagcacgaca ccgggaatcc acagttgcag gcccagcccg acagcggcgg ccaacgtgcc
     2461 gttggcgatc gccacggtgc gcggtgcgcc gcccagcagg atcggctcgg tgagcgagcg
     2521 atgcaaaggc acctcgaacc cggccgcgaa ggtgtccggg ccgctcatac gacggccccg
     2581 cccgagaagc tgaagaacga caggaagaag ctcgaagccg cgaacgcgat ggacaagcca
     2641 aagacgatct ggatgagctt gcggaagccg ccggacgtgt cgccgaacgc gagcgccagg
     2701 cccgtggcga tgatgatgat gaccgcgacg atgcgcgcca ccggcccctg ga
//
LOCUS       DAOMTQ010000258         2702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11076_length_2702_cov_25.803929, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000258 DAOMTQ010000000
VERSION     DAOMTQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11076_length_2702_cov_25.803929"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PK518_15475"
     CDS             <1..1016
                     /locus_tag="PK518_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM50358.1"
                     /translation="VTLAFGTILWPLNGAIFWGVVLAILFSPLHRRLQRRMPRRRNLA
                     AFVTLCICLVIVILPMALIGLSLVQEASVAYERVRSGQLNYGAYLQQMLAALPAWVLE
                     LLDRFHLTSMAELQARLTAFGAQASQFLATKALDLGQNTLQFVVSFGVMLYLLFFLLR
                     DGRALAARIREAIPLDAEHKDQLAQKFTTVIRATVKGNIVVAASQGALGGLIFWLLDI
                     QGPVMWGVLMAFLSLLPAVGASLIWGPVAIYFLATGATGQAAILTAYGIGVIGLVDNL
                     LRPLLVGKDTKMPDYVVLISTLGGMALFGLTGFVIGPVIAALFMATWGLFAPAGDSHS
                     SQK"
     gene            complement(1078..2064)
                     /locus_tag="PK518_15480"
     CDS             complement(1078..2064)
                     /locus_tag="PK518_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM50359.1"
                     /translation="MNPSMHRRRALAIVAATLACAAAGPASAQAPAYPAKAITFIVPF
                     AAGSATDQLARALGQSVTTDTKQPVVVDNKAGASGMIAAQAAAKAPADGYTVLITTNT
                     THAANEHLYKKLSYDPVKDFAPVTGLGKGGQVLVVNAGAPYKSVAELLAFAKKNPGKL
                     SFGSGSSSSRMAGEMLKQLADVDILHVPYKSNPLAITDLLGGQIDMMITDTSTGVPQI
                     KAGKLRALGYSTQKRGAQLPDVPTIAEAGVKGYDMGYWFAAYVPAGTPAPVVARLNEL
                     LAHATKSEAARSFFTNAGSEAWTTTPEELAKFQAAETQKWGKVIKAAGIEPE"
     gene            complement(2107..2469)
                     /locus_tag="PK518_15485"
     CDS             complement(2107..2469)
                     /locus_tag="PK518_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50360.1"
                     /translation="MTDTVLTVPLHRLAHGRTGDKGNRSNISVIAWHPALWDVLVDQV
                     TEEAVARQFAERRPSQVTRHVLPKLQAMNFVLDGVLDGGVNDALNLDSHGKALSFLLL
                     DLPVQVPAALAQHLAGPR"
     gene            complement(2484..>2702)
                     /locus_tag="PK518_15490"
     CDS             complement(2484..>2702)
                     /locus_tag="PK518_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50361.1"
                     /translation="QAPGDARDVRLRVALQHTEHASAQRLVREVTALYCCGPAGGGGV
                     RTAMRPRLGTVSCLVPRAAVTTGFTLLD"
BASE COUNT          381 a          878 c          933 g          510 t
ORIGIN      
        1 tcgtcacgct ggcctttggc accatcctct ggccgctcaa tggcgccatt ttctggggag
       61 tggtgctggc cattctgttc agcccgttgc accgccgctt gcagcggcgc atgccacggc
      121 ggcgcaacct ggcggccttc gtcacgctgt gcatctgcct ggtgatcgtc atcctgccca
      181 tggcgctcat aggactgtcg ctggtgcagg aggccagcgt ggcctatgag cgggtgcgct
      241 ccgggcagct gaattacggc gcctacctgc agcagatgct ggccgccctg cccgcctggg
      301 tgctggagct gctggaccgc ttccacctca cctccatggc cgagctgcag gcgcgcctga
      361 cggcctttgg cgcccaggcc agccagttcc tggccaccaa ggcgctggac ctggggcaga
      421 acacgctgca gttcgtcgtc agctttggcg tcatgctgta cctgctgttc tttctgctgc
      481 gcgacgggcg ggcactggcg gcgcgcatcc gcgaggcgat tccgctggac gccgagcaca
      541 aggatcagct ggcgcagaag ttcaccaccg tgatccgtgc cacggtcaag ggcaacatcg
      601 tcgtcgccgc ctcgcagggc gcgctgggcg gactgatctt ctggctgctg gacatacagg
      661 ggccggtgat gtggggcgtg ctgatggcct ttctgtcgct gctgccggcc gtgggcgcca
      721 gcctgatctg gggcccggtc gccatctact tcctggccac cggcgccacc ggccaggccg
      781 ccatcctcac ggcctacggc atcggcgtca tcggcctggt ggacaacctg ctgcgcccgc
      841 tgctggtggg caaggacacc aagatgccgg actacgtggt actgatctcc acgctgggcg
      901 gcatggcgct gtttggcctg accggcttcg tcatcggccc cgtgattgcc gccctgttca
      961 tggccacctg gggcttgttt gcccccgccg gggactcaca tagctcccaa aagtagagct
     1021 actcgcgctt atccataaag cgcttgaagc caaaaatatt caaaattcat cgcccactca
     1081 ttcgggctct atgcccgcgg ccttgatgac cttgccccat ttctgggtct cggcggcctg
     1141 gaatttggcg agctcctcgg gcgtggtggt ccaggcttcg gagccggcgt tggtgaagaa
     1201 cgacctggcg gcctcgcttt tcgtggcgtg ggccagcagc tcgttcaggc gcgccaccac
     1261 cggcgcgggc gtgccggcgg gcacgtaggc ggcgaaccag tagcccatgt cgtagccctt
     1321 cacgccagcc tcggcgatgg tgggtacgtc gggcagctgg gcgccgcgct tttgcgtgga
     1381 gtagcccagg gcgcgcagct tgccggcctt gatctgcggc acgccggtag aggtgtcggt
     1441 gatcatcatg tcgatctgcc cgcccagcag gtcggtaatc gccagcgggt tgctcttgta
     1501 gggcacatgc aggatgtcca catcggccag ctgcttgagc atctcgccgg ccatgcggct
     1561 cgatgagctg ccgctgccaa agctgagctt gccggggttc ttcttggcaa aggccagcag
     1621 ctcggccacg cttttgtagg gcgcgccagc attcaccacc agcacctgcc cacccttgcc
     1681 caggccggtg acgggcgcga agtctttcac cgggtcgtag gacagctttt tgtacaggtg
     1741 ctcgttggca gcgtgcgtgg tgttggtggt gatgagcacg gtgtagccgt cggctggcgc
     1801 cttggccgcc gcctgggcgg cgatcatgcc gctggcgccg gccttgttgt ccaccaccac
     1861 gggctgcttg gtgtcggtgg tgaccgactg gcccagggcg cgcgccagct ggtcggtggc
     1921 gctgccggcg gcaaacggca cgatgaaggt gatggccttg gccggatagg ccggtgcctg
     1981 cgcgctggcc gggccggccg cggcgcatgc cagcgtggcg gcaacgatag ccagtgcgcg
     2041 gcggcggtgc atggatgggt tcatgtttgt ctcctgggtt tgctgtgttg aaaatcgaaa
     2101 aatggcttac cgggggccag ccaggtgctg cgccagtgcc gccggcacct gcaccggcag
     2161 atcgagcagc aagaaggaca gggccttgcc atggctgtcg aggttcagcg cgtcgttgac
     2221 gccgccgtcg agcacgccgt cgagcacgaa gttcatggcc tgcagcttgg gcaacacatg
     2281 gcgcgtgacc tggctgggcc gtcgctctgc aaactggcgc gccacggcct cttcggtgac
     2341 ttgatcgacc agcacatccc agagcgcggg gtgccaggcg atgacgctga tgttggagcg
     2401 attgcccttg tcgcccgtac ggccgtgggc caggcggtgc aggggaacgg tgagaacggt
     2461 atcggtcatg gttattgcac ctttcaatcc agcagcgtga aaccggtggt gacggccgcg
     2521 cgcggcacca ggcaggacac cgtgcccagg cgcggccgca tggcggtgcg cacgccgccg
     2581 ccgccggccg gaccgcagca gtacagcgcc gtcacctcgc gcaccaggcg ctgggcgctg
     2641 gcgtgctcgg tgtgttgcag cgccacgcgc aggcgcacgt cgcgcgcatc gccgggcgcc
     2701 tg
//
LOCUS       DAOMTQ010000259         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11428_length_2644_cov_30.446504, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000259 DAOMTQ010000000
VERSION     DAOMTQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11428_length_2644_cov_30.446504"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..836
                     /locus_tag="PK518_15495"
     CDS             57..836
                     /locus_tag="PK518_15495"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRM50362.1"
                     /translation="MAYECITVRTEGEKVGVITLNRPKQLNALNDQLMNELGQALQAF
                     DADESIGCIILTGSEKAFAAGADIGAMAKYSFADAYKGDYITRNWEAIRSIRKPVIAA
                     VSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKSKAMDM
                     CLTARMMDAQEAERAGLVSRVVPLDKMMDEALGAALIISGFSQIAVMAAKESVNRAFE
                     GTLADGVMFERRLFHALFATQDQKEGMDAFVNKRAANFTHQ"
     gene            874..950
                     /locus_tag="PK518_15500"
     tRNA            874..950
                     /locus_tag="PK518_15500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:908..910,aa:Met,seq:cat)
     gene            1025..1423
                     /locus_tag="PK518_15505"
     CDS             1025..1423
                     /locus_tag="PK518_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50363.1"
                     /translation="MQRCTLPALAIAAMLALPVAVHAQPTPAGQRHFPEAALRGQLTI
                     HSSAEARLNNQSVRLAPGLRIFNTSNALVFAHSLINQPLKVNYVIEASTGMLLTAWIL
                     TEDEARQPRKGSDGVQNNIRTESHTPQTAR"
     gene            1434..>2644
                     /gene="miaB"
                     /locus_tag="PK518_15510"
     CDS             1434..>2644
                     /gene="miaB"
                     /locus_tag="PK518_15510"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRM50364.1"
                     /translation="MSKKVFIKTFGCQMNEYDSAKMADVLGAAQDYVETSNPEEADLI
                     LFNTCSVREKAQEKVFSDLGRVKHLKEKGVLIGVGGCVASQEGEEIIKRAPFVDVVFG
                     PQTLHRLPDLLNARQKQHKPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCS
                     YCVVPYTRGEEVSRPFEDLLVEVAGLADQGVKEVTLLGQNVNAYLGKMGDTQDTADFA
                     LLLEYVAEIPGIERIRYTTSHPNEFTPRLIEAYAKIPKLVSHLHLPVQHGSDRILMAM
                     KRGYTAMEYKSTVRKLKAIRPDMAMSSDFIVGFPGETEEDFQKMMKLIHDVRFDNSFS
                     FIFSPRPGTPAANLADDTPHDVKLRRLQELQAVINRNILEISQERVGTVQRLLVEGLS
                     KRDGNELMGRT"
BASE COUNT          563 a          914 c          771 g          396 t
ORIGIN      
        1 agcttggtag cttggttata gtcaaccggt tcaacccacc cataccggag atccccatgg
       61 cctacgaatg catcaccgtc cgcaccgagg gcgagaaggt cggcgtcatc acgctgaacc
      121 gccccaagca gctcaacgcc ctgaacgacc agctcatgaa cgagctgggc caggccctgc
      181 aggcctttga cgccgacgag tccatcggct gcatcatcct caccggcagc gagaaggcct
      241 tcgccgccgg cgccgacatc ggcgccatgg ccaagtacag ctttgccgac gcctacaagg
      301 gcgactacat cacgcgcaac tgggaggcca tccgcagcat caggaagccg gtgatcgccg
      361 ccgtcagcgg ctttgccctg ggcggcggtt gcgagctggc catgatgtgc gacttcatca
      421 tcgccgccga caacgccaag ttcggccagc ccgagatcaa gctgggcgtc atccccggcg
      481 cgggcggcac gcagcgcctg ccgcgcgccg tgggcaagtc caaggccatg gacatgtgcc
      541 tgaccgcccg catgatggac gcccaagagg ccgaacgcgc cggcctggtc agccgcgtcg
      601 tgccgctgga caagatgatg gacgaggccc tgggcgccgc cctgatcatc agcggcttct
      661 cgcagatcgc cgtcatggcc gccaaggagt cggtgaaccg cgcgttcgaa ggcacgctgg
      721 ccgacggcgt gatgtttgag cgccgcctgt tccacgcgct gttcgccaca caagaccaga
      781 aggaaggcat ggacgccttc gtgaacaagc gcgcggcaaa tttcacgcac cagtgatggc
      841 acgccaaatt ttcgttctat aattcgcggc ttcggtggta tagctcagtt ggttagagcg
      901 cagcattcat aatgctgatg tcccaggttc aagtcccggt accaccacca cacaagaaaa
      961 acccgcagtg cctcaggtgc tgtgggtttt ttgcattgat agcaacagcc cctggataca
     1021 caccatgcaa cgctgcaccc tccccgcatt ggccatcgcc gccatgctgg ccctgcccgt
     1081 ggccgtccac gcgcagccca cgcccgccgg ccagcgccac tttcccgagg ccgcgctgcg
     1141 cggtcagctg accattcaca gcagcgccga ggcgcgcctg aacaaccagt cagtgcgcct
     1201 ggcgccaggc ctgcgcatct tcaacaccag caacgcgctg gtgtttgcgc acagcctgat
     1261 caaccagccg ctcaaggtga attacgtgat cgaggccagc accggcatgc tgctcacggc
     1321 ctggatcctg accgaggacg aagccaggca gccgcgcaag ggcagcgacg gcgtgcagaa
     1381 caacatccgc accgagtccc acaccccgca aaccgcacgc tgaaggtcgg atcatgagca
     1441 aaaaagtctt catcaaaacc tttggctgcc agatgaacga gtacgactcg gccaagatgg
     1501 ccgacgttct gggcgcagcg caggactatg tggaaaccag caaccccgaa gaggccgacc
     1561 tgatcctctt caacacctgc tcggtccgcg agaaggcgca agaaaaagtc ttcagcgacc
     1621 tgggccgcgt caagcacctc aaggaaaaag gcgtgctcat cggcgtgggc ggctgcgtcg
     1681 ccagccagga gggcgaggag atcatcaagc gcgcaccctt cgtggacgtg gtcttcggcc
     1741 cgcagaccct gcaccgcctg cccgacctgc tgaacgcgcg ccagaagcaa cacaaacccc
     1801 aggtggacat cagcttcccc gagatcgaga agttcgacca cctgccgccc gcgcgcgtcg
     1861 agggcgcaag cgccttcgtc tccatcatgg agggctgctc caaatactgc agctactgcg
     1921 tggtgcccta cacgcgcggc gaggaggtca gccgcccgtt cgaggacctg ctggtggagg
     1981 tcgcgggcct ggccgaccag ggcgtgaagg aggtcacact cctgggccag aacgtcaacg
     2041 cctacctggg caagatgggc gacacgcaag acacagccga ctttgccctg ctgctggaat
     2101 acgtggccga gataccgggc atagagcgca tccgctacac caccagccac cccaacgagt
     2161 tcacgccgcg cctgatcgag gcctacgcca aaatcccgaa gctggtcagc cacctgcacc
     2221 tgcccgtgca gcatggcagc gaccgcatcc tgatggccat gaagcgcggc tacaccgcca
     2281 tggaatacaa gagcacggtg cgcaagctca aggccatacg ccccgacatg gccatgagca
     2341 gcgacttcat cgtcggcttt cccggcgaga ccgaggaaga cttccagaaa atgatgaagc
     2401 tcattcacga cgtgcgcttc gacaactcct tcagcttcat cttcagcccc cgccccggca
     2461 cccccgccgc caacctggcc gacgacacgc cgcacgacgt gaaactgcgc cgcctgcaag
     2521 aactgcaggc cgtcatcaac cgcaacatcc tggaaatcag ccaggaacgc gtgggcacgg
     2581 tgcagcgcct gctggtcgag ggcctctcca aacgcgacgg caacgagctc atgggccgca
     2641 ccga
//
LOCUS       DAOMTQ010000260         2635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11488_length_2635_cov_21.569767, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000260 DAOMTQ010000000
VERSION     DAOMTQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11488_length_2635_cov_21.569767"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PK518_15515"
     CDS             complement(<1..470)
                     /locus_tag="PK518_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRM50365.1"
                     /translation="MASTINTNVASLTAQRNLGISQNSLNTSIQRLSSGLRINSAKDD
                     AAGLAISERFTSQIRGLNQAVRNANDGISLAQTAEGALKSTGDILQRVRELSVQSANA
                     TNSAGDRKAIQAEVGQLLSEMDRISQTTEFNGQKLLDGSFGSATFQVGANANQT"
     gene            812..2290
                     /locus_tag="PK518_15520"
     CDS             812..2290
                     /locus_tag="PK518_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRM50366.1"
                     /translation="MASTINTNVASLTAQRNLGVSQGSLNTSIQRLSSGLRINSAKDD
                     AAGLAISERFTAQIRGLNQAARNANDGISLSQVAESALAGAGNILQRVRELSVQSANA
                     TNSASDRKAIQAEVGQLLSELDRMAVTTEFNGQKLLDGSFGSATFQVGANANQTITAT
                     TGNFRTATYGTQLKQSAPIVVTAAAAPTALTGTFDVAGLQTKTVTLTATDTMATAAAK
                     INALTEATGVSATARNQVELSNFAAGGSYSLAVKGDNSTAANVTFSVSAAGATAAGLA
                     ESIKAFNDASSQTGITAKLNANGNGVVLINESGADIQVQNNSAAGNTISLGAYDAAAS
                     ATSGTSTFVPGVAAAPAGGFATARGYLEMNSDKGFSIGNLGGTPPVTATASTLNSVNT
                     IDVSTVDGSTKALKIIDSALAAVNGQRASFGALQARFETAIANLNTSSENMSASRSRI
                     QDADFAQETANLSRTQILQQAGTAMVAQANQIPQGVLSLLKG"
     gene            2449..>2635
                     /locus_tag="PK518_15525"
     CDS             2449..>2635
                     /locus_tag="PK518_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596656.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HRM50367.1"
                     /translation="MFVILGYLITFGCIFGVFIAHGGNIKVVLEALPFEMITIGGAAA
                     GAFIINNQPKVLKATMGA"
BASE COUNT          482 a          891 c          811 g          451 t
ORIGIN      
        1 atggtctgat tggcattcgc tcccacctgg aaggtggccg agccgaacga gccgtccagc
       61 agcttctggc cgttgaactc ggtggtctgc gagatgcggt ccatttcgga cagcagttgg
      121 cccacttcgg cctggatggc cttgcggtcg ccggcgctgt tggtggcgtt ggccgactgc
      181 accgacagtt cgcgcacgcg ctgcaggatg tcgccggtgc tcttgagcgc gccttccgcc
      241 gtctgcgcca gcgagatgcc gtcgttggcg ttgcgcacgg cctggttcag gccgcggatc
      301 tggctggtaa agcgctccga aatcgccagg ccggcggcgt cgtccttggc gctgttgatg
      361 cgcaggcccg acgacaggcg ctggatggag gtgttgagcg aattctggct gatgcccagg
      421 ttgcgctggg cggtgagcga ggcgacgttg gtgttgatgg tggaagccat ggcaaatctc
      481 ctgaaaagta ccgattggat atggcgttgc cgccaactgg gcccagaacc ttctggccac
      541 agcgttcaca gtgccaccca ggctgcctgt tgaacggcgg gtcgtcactt cttgtttcaa
      601 acggcccgtt gaatgtgttt tcgggttgtg gccgaaaaac tttaggggcg tccggccgaa
      661 tcggcggatt gggcggcttt tgcgccgttt atccagccaa cgcggcggcg gcagctgccc
      721 acaatcaccc catcgttccc gcaaagtcgg ccgctggagc aaggcctgag ccggcaacca
      781 cacttttacc cagccacgca ggagttccgc catggcatcc acgatcaaca ccaacgtcgc
      841 ctcgctcacc gcccagcgca acctgggcgt gagccagggg tcgctcaata cctccatcca
      901 gcgcctgtcg tcgggcctgc gcatcaacag cgccaaggac gacgcggcgg gcctggcgat
      961 ctccgagcgc ttcacggccc agatccgcgg cctgaaccag gcggcgcgca acgccaacga
     1021 cggcatctcg ctgtcgcagg tggcagaaag cgccctggcc ggcgccggca acatcctgca
     1081 gcgcgtccgc gaactgtcgg tgcagtcggc caacgccacc aactcggcca gcgaccgcaa
     1141 ggccatccag gccgaggtcg ggcagctgct gtcggagctg gatcgcatgg ccgtgaccac
     1201 cgagttcaac ggccaaaagc tgctggatgg ctcgttcggc tcagccacct tccaggtggg
     1261 ggcgaatgcc aaccagacca tcactgccac caccggcaac tttcgcactg ctacctatgg
     1321 cacgcagctc aagcagtcgg cgcctatcgt cgtcacagca gcagcggcac ccaccgccct
     1381 gaccggtacc ttcgacgtgg cggggctgca gaccaagacc gtgaccctga cggccacgga
     1441 caccatggcg acggccgcgg ccaagatcaa tgcattgacg gaggccacgg gggtgagcgc
     1501 cacggcgcgc aaccaggtcg agctgagcaa ctttgccgcc ggcggctcct actccctggc
     1561 cgtcaagggc gacaacagca cggccgccaa cgtgaccttc agcgtcagcg ctgcgggcgc
     1621 cactgcggcg ggcctggcgg agagcatcaa ggccttcaac gacgcgtcgt cgcagaccgg
     1681 catcacggcc aagctcaatg ccaatggcaa tggcgtggtg ctgatcaacg agtcgggcgc
     1741 cgacatccag gttcagaaca attccgctgc gggcaacact atctccctgg gcgcctacga
     1801 tgccgcagcc tccgccactt cgggtacctc gacctttgtg cccggcgtgg cggcggcacc
     1861 agcaggtggt tttgctactg cgcgcggcta tctggagatg aattcggaca agggcttctc
     1921 catcggcaac cttggaggca caccgccggt gaccgccaca gcttcgacgc tcaactcagt
     1981 gaacaccatc gacgtctcca ccgtggacgg ctccaccaag gccctgaaga tcatcgactc
     2041 cgccctggcc gccgtgaacg gccagcgtgc cagctttggc gcactgcagg cacgctttga
     2101 gacggccatc gccaacctga acacgtcgtc ggagaacatg tccgcctcgc gcagccgcat
     2161 ccaggatgcg gactttgccc aggaaaccgc caacctgtca cgcacccaga tcctgcagca
     2221 ggccggcacg gccatggtgg cccaggcgaa ccagattccg cagggcgtgc tgtccctgct
     2281 caagggttga gcggcctggc ggccgtatcg gcagcaggca ccctttgagg catcggccat
     2341 gcgcttcccc atgccttgac cagattcaca atcagataca gtacatccct catacagggt
     2401 atgcctgcca cgcttgaccc aaggctgcca tagcaacccg gatccaccat gttcgtcatc
     2461 ctcggctacc tcatcacctt cggctgcatc ttcggcgtgt tcatcgccca tggcgggaac
     2521 atcaaggtgg tgctggaggc actacccttc gagatgatca ccatcggtgg cgcggcagcc
     2581 ggggcgttca tcatcaacaa ccagcccaag gtgctcaagg ccaccatggg cgcgc
//
LOCUS       DAOMTQ010000261         2628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11533_length_2628_cov_21.455499, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000261 DAOMTQ010000000
VERSION     DAOMTQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11533_length_2628_cov_21.455499"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PK518_15530"
     CDS             <1..321
                     /locus_tag="PK518_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRM50368.1"
                     /translation="LELAPRLPLAAASAADLTVGLRASALRVQARPGDVAVQGQVELA
                     EISGSDTFVHVATPMGEVVAQLTGVHYFALGSTLSLYFDPAQVYVFAGHGALLLAPAR
                     MGGH"
     gene            323..1399
                     /locus_tag="PK518_15535"
     CDS             323..1399
                     /locus_tag="PK518_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM50369.1"
                     /translation="MAHITLDLAHSYKPRPQQDSDYALLPLAMEFQDGGAYALLGPSG
                     CGKTTMLNIMSGLLVPSHGKVLFDGHDVTRATPQDRNIAQVFQFPVIYDTMTVAENLA
                     FPLKNRKLPEARIRERVGRIAEMLEMSGQLDQRAAGLAADAKQKISLGRGLVREDVAA
                     VLFDEPLTVIDPHLKWQLRRKLKQIHHELKLTLIYVTHDQVEALTFADQVVVMSRGKV
                     MQLGTPDALFERPQHQFVGHFIGSPGMNFLPAQSAAGRLQVAGHALEQPAGRGLPEGA
                     LQVGVRPEYLALAQPGAAGALPCTVQRVQDIGTYQMLTAQVGEHLLRARFAPEQRLPA
                     PGETAWLQVLGAHTCYYQNEELLP"
     gene            1396..2292
                     /locus_tag="PK518_15540"
     CDS             1396..2292
                     /locus_tag="PK518_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM50370.1"
                     /translation="MSATTKPVNQKAWFLILPVLLCVAFSAIVPLMTVVNYSVQDIIS
                     PERRVFVGTEWFAAVMRDEELHAALWRQLTFSLAVLLVEIPLGILLALSMPAQGWKAS
                     AVLVLVALSLLIPWNVVGTIWQIYGRTDIGLLGAALQSMGIDYNYTGHASDAWVTVLV
                     MDVWHWTPLVALLCYAGLRSIPDAYYQAARIDGASKLAVFRYIQLPKMRGVLMIAVLL
                     RFMDSFMIYTEPFVLTGGGPGNATTFLSQYLTQKAVGQFDLGPAAAFSLIYFLIILLL
                     CFILYNWMQRVGTTAKEGAGHE"
     gene            2285..>2628
                     /locus_tag="PK518_15545"
     CDS             2285..>2628
                     /locus_tag="PK518_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM50371.1"
                     /translation="MNDKRFQKRTLFLIAYLVFAILPIYWMVNMSFKTNEEILSSFSL
                     WPQHFTWDNYKTIFTDESWYSGYINSLIYVAINMVIALTVALPAAYAFSRYQFLGDKH
                     VFFWLLTNRMTPP"
BASE COUNT          405 a          915 c          866 g          442 t
ORIGIN      
        1 ctggagctgg cgccgcgcct gccgctggcc gcggccagcg ccgccgacct gaccgtgggc
       61 ctgcgcgcca gcgcgctgcg cgtgcaggcg cggccggggg atgtggcggt gcagggccag
      121 gtggagctgg cagagatctc cggctccgac accttcgtgc acgtggccac gcccatgggc
      181 gaggtggtgg cgcagctcac cggcgtgcac tactttgccc tgggcagcac gctcagcctg
      241 tatttcgacc cggcgcaggt ctacgtgttc gccggccatg gcgccctgct gctggcgccg
      301 gcgcgcatgg gagggcactg acatggcgca tatcacgctg gatctggcgc actcgtacaa
      361 gccgcgcccg cagcaggaca gcgactacgc cctgctgccc ctggcgatgg agttccagga
      421 cggcggcgcc tacgcgctgc tggggccctc gggctgcggc aagacgacca tgctcaacat
      481 catgtcgggc ctgctcgtgc cctcgcacgg caaggtgctg ttcgacgggc acgacgtcac
      541 gcgcgccacg ccgcaggacc gcaacatcgc ccaggtgttc cagttcccgg tcatctacga
      601 caccatgacc gtggccgaga acctggcctt cccgctcaag aaccgcaagc tgcccgaggc
      661 gcgcatccgc gagcgcgtgg gcaggatcgc cgagatgctg gagatgagcg gccagctcga
      721 ccagcgcgcc gcaggcctgg cggccgatgc caagcagaag atctcgctgg gccgcggcct
      781 ggtgcgcgag gacgtggcgg ccgtgctgtt cgacgagccg ctcaccgtca tcgacccgca
      841 cctcaagtgg caactgcggc gcaagctcaa gcagatccac cacgagctca agctcacgct
      901 gatctacgtc acgcacgacc aggtcgaggc gctcaccttc gccgaccagg tggtggtcat
      961 gagccgcggc aaggtcatgc agctgggcac gcccgatgcg ctgttcgagc gcccgcagca
     1021 ccagttcgtc gggcacttca tcggctcgcc gggcatgaac ttcctgcccg cgcagagcgc
     1081 ggccggccgc ctgcaggtgg cgggccacgc gctggagcag cccgcggggc gcggcctgcc
     1141 cgaaggcgcg ctgcaggtgg gcgtgcgccc cgagtacctg gcgctggccc agcccggggc
     1201 ggcgggcgcc ctgccgtgca ccgtgcagcg cgtgcaggac atcggcacct accagatgct
     1261 caccgcgcag gtgggcgagc acctgctgcg tgcgcgcttt gctcccgagc agcgcctgcc
     1321 cgcgccgggc gagacggcct ggctgcaggt gctgggcgcg cacacctgct actaccagaa
     1381 cgaggagctg ttgccatgag tgcaacgacc aaaccggtga accagaaggc ctggttcctc
     1441 atcctgcccg tgctgctgtg cgtggcgttc tcggccatcg tgcccctgat gacggtggtc
     1501 aactactcgg tgcaggacat catctcgccc gagcgccgcg tgttcgtggg caccgagtgg
     1561 tttgccgccg tgatgcgcga cgaggaactg cacgcggccc tgtggcgcca gctcaccttc
     1621 tcgctggccg tgctgctggt ggagatcccg ctgggcattc tgctggccct gtccatgccg
     1681 gcgcagggct ggaaggcctc ggccgtgctg gtgctggtgg cgctgtcgct gctcatcccg
     1741 tggaacgtgg tgggcaccat ctggcagatc tatggccgca ccgacatcgg cctgctgggc
     1801 gcggcgctgc agtccatggg catcgactac aactacaccg gccatgccag cgacgcctgg
     1861 gtcaccgtgc tcgtgatgga cgtgtggcac tggacgccgc tggtggcgct gctgtgctac
     1921 gcgggcctgc gctccattcc cgatgcgtac taccaggcgg cgcgcatcga cggcgcgagc
     1981 aagctggcgg tgttccgcta catccaactg cccaagatgc gcggcgtgct gatgatcgcc
     2041 gtgctgctgc gcttcatgga cagcttcatg atctacaccg agcccttcgt gctcacgggc
     2101 ggcgggccgg gcaatgcgac cacgttcctg agccagtacc tcacgcaaaa ggccgtgggc
     2161 cagttcgacc tggggccggc ggccgccttc tcgctgatct acttcctcat catcctgctg
     2221 ctgtgcttca tcctctacaa ctggatgcag cgcgtgggca ccacggccaa ggagggcgcg
     2281 ggccatgaat gacaagcgct tccagaaacg cacgctgttt ctcatcgcct acctggtgtt
     2341 cgccatcctg cccatctact ggatggtgaa catgagcttc aagaccaacg aggagatcct
     2401 gtcgagcttc tcgctctggc cccagcattt cacctgggac aactacaaga ccatcttcac
     2461 cgacgagagc tggtactcgg gctacatcaa cagcctgatc tacgtggcca tcaacatggt
     2521 catcgccctc accgtggcgc tgcccgcggc ctatgccttc agccgctacc agttcctggg
     2581 cgacaagcat gtgttcttct ggctgctgac caaccgcatg acgccgcc
//
LOCUS       DAOMTQ010000262         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11569_length_2622_cov_28.316323, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000262 DAOMTQ010000000
VERSION     DAOMTQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11569_length_2622_cov_28.316323"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="gidA"
                     /locus_tag="PK518_15550"
     CDS             <1..636
                     /gene="gidA"
                     /locus_tag="PK518_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465095.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRM50372.1"
                     /translation="AEFRLQLREDNADMRLTEAGRRMGLVDDARWDAFSRKRDAVSRE
                     TERLKATWVSPRILAAAESERVLGKAIEHEYNLFDLLRRPDVGYASLMSLDGGKYASS
                     AVSRETLGDLSAPVVEQVEIAAKYSGYIDRQKDEVQRAAHFEKLCLPGDLDYMQVAAL
                     SIEVRQKLQKHRPETLGQASRISGVTPAAISLLMVHLKKGGFKGFAAEATA"
     gene            633..1286
                     /gene="rsmG"
                     /locus_tag="PK518_15555"
     CDS             633..1286
                     /gene="rsmG"
                     /locus_tag="PK518_15555"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRM50373.1"
                     /translation="MSAPLDVALRAGCQTLGLELDEAQIAKLLDFMGLLQKWNKVYNL
                     TAVRNPEEMLTHHLLDSLAAVAPLRRHLAQQGGAGRRLLDVGSGGGLPGVVFAICCPD
                     LDVSCVDTVAKKAAFIQHAAAALQLSNLHGLHARVENLDGPFDVVSCRAFAALADFTA
                     WSRQALAPHGVWLAMKGRHPQDEIDALPGDVQVFHVEQLTVPGLQAERCIVWLRPVA"
     gene            1325..1939
                     /locus_tag="PK518_15560"
     CDS             1325..1939
                     /locus_tag="PK518_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRM50374.1"
                     /translation="MFGIADYGAFVAAIVLFLAIPGPGNLAIITSTAKGGVRAGMAAC
                     FGVIAADQALMWLAVAGVAGLLAAYPAAFAAVQWLGAAYLGWMGYKMLTAKPGQAPVV
                     NIKPRHYFRQGAVITLLNPKAIVFYMAFFPLFVNPATHRGLVTFAAMAATIAVLTFLY
                     CLVVVLLTQHMAERMRANPTVVRTLEKLAGVFLIGFGVKLAISK"
     gene            1955..>2622
                     /locus_tag="PK518_15565"
     CDS             1955..>2622
                     /locus_tag="PK518_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM50375.1"
                     /translation="MAKIFCIANQKGGVGKTTTSVNLAAGLAKIGQRVLLVDLDPQGN
                     ATMGSGVDKRQLALSVYDVLLESTSVKEAAVLAEPCAYHVLGANRELAGAEVELVPLE
                     HREKRLKAALAEVDQDYDFVLIDCPPSLSMLTLNGLCSANGVIVPMQCEYFALEGLTD
                     LVNTIKQVHANLNRDLQIIGLLRVMFDPRITLQNQVSAQLKDHFGDKVFDTVIPRNVR
                     LAEAP"
BASE COUNT          437 a          849 c          875 g          461 t
ORIGIN      
        1 gccgagttcc gcctgcagct gcgcgaggac aacgccgaca tgcgcctgac cgaggccggg
       61 cgccgcatgg gcctggtgga cgacgcgcgc tgggatgcat tcagccgcaa acgcgatgcg
      121 gtttcacgtg aaaccgagcg cctgaaagcc acctgggtga gcccgcgcat cctcgccgcg
      181 gccgagtccg agcgcgtgct gggcaaggcc atagagcatg agtacaacct gttcgacctg
      241 ctgcgccgcc cggacgtggg ctacgccagc ctcatgagcc tggacggtgg caagtacgcc
      301 agcagtgctg tttcacgtga aaccctgggc gatctgagcg cgccggtggt ggagcaggtg
      361 gagattgccg ccaagtattc gggctacata gaccggcaaa aggacgaggt gcagcgcgcc
      421 gcgcatttcg agaagctgtg cctgccgggc gacctggact acatgcaggt ggcagcgctg
      481 tccatcgagg tgcgccagaa gctgcaaaag caccggcccg agactctggg ccaggcgtcc
      541 cgcatctccg gcgtgacgcc ggcggcgatc tcgctgctca tggtgcatct gaagaagggc
      601 ggcttcaagg gctttgccgc ggaggccacg gcatgagcgc gccgctcgat gtggcgctgc
      661 gcgcaggttg ccagaccctg ggcctggagc tcgatgaggc gcaaatcgcc aagctgctgg
      721 acttcatggg cctgctgcaa aagtggaaca aggtctacaa cctgacggcc gtgcgcaacc
      781 ccgaggagat gctgacgcac cacctgctcg acagcctggc ggccgtggcg ccgctgcgcc
      841 gccacttggc gcagcagggc ggggcggggc ggcgcctgct ggacgtgggc agcggcggag
      901 gcctgccggg cgtggtgttt gccatctgct gccccgacct ggatgtcagc tgcgtggata
      961 ccgtggccaa gaaggcggcc ttcatccagc acgcggcagc ggcgctgcaa ctgtcaaacc
     1021 tgcacggcct gcatgcccgg gtggaaaacc tggatggccc gttcgacgtt gtgagctgcc
     1081 gcgccttcgc cgcgctggcg gacttcaccg cctggtcgcg ccaggcgctg gcgccacatg
     1141 gcgtgtggct ggccatgaag gggcggcacc cgcaggacga gatcgacgcg ctgcccggtg
     1201 acgtacaggt gtttcacgtg gaacagctca cagttccggg tttgcaggca gagcgctgca
     1261 tcgtctggct gcgacccgtc gcttaaactc gagcgtttcc ttcttcgctc gcagcgctcc
     1321 tcccatgttt ggcattgctg actacggcgc tttcgtcgcc gccatcgtgt tgtttctggc
     1381 gattcccggg ccgggcaacc tggcaatcat cacctcgacg gccaagggtg gggtgcgcgc
     1441 cggcatggcc gcatgcttcg gggtcattgc ggcagaccag gcactgatgt ggctggccgt
     1501 ggccggcgtg gctggcctgc tggcggccta cccggcggcg tttgcggccg tgcaatggct
     1561 gggcgcggcg tacctgggct ggatgggcta caagatgctt accgccaagc ccggccaggc
     1621 gccggtcgtg aacatcaagc cgcgccatta ttttcgccag ggggcggtga tcacgctgct
     1681 caaccccaag gccatcgtgt tctacatggc cttcttcccc ttgttcgtga acccggcgac
     1741 gcaccgtggg ctggtgacct tcgccgccat ggcggccacc atcgccgtac tcactttttt
     1801 gtattgcctg gtggtggtgc tgctgaccca gcacatggcc gagcgcatgc gcgccaaccc
     1861 cacggtggtg cgcacgctgg agaagctggc gggcgttttc cttatcggct ttggcgtcaa
     1921 gctggccatc tccaagtaaa ccggacggga ctgcatggcc aagatcttct gcattgccaa
     1981 ccaaaagggt ggcgttggca agaccactac ctccgtcaac ctcgccgccg ggctggccaa
     2041 aatcggccag cgcgtgctgc tggtggattt ggatccgcaa ggcaatgcca ccatgggctc
     2101 gggcgtggac aagcgccagc ttgcgctgag cgtgtatgac gtgctgctcg aatccacctc
     2161 cgtcaaggaa gccgccgtgc tggccgaacc ctgcgcctac catgtgctgg gcgccaaccg
     2221 cgaactggcc ggcgccgagg tcgagctggt gccattggag caccgtgaaa agcgcctcaa
     2281 ggcggcgctg gccgaggtgg atcaggacta cgacttcgtg ctcatcgact gcccgccgtc
     2341 gctatccatg ctgacgctca acggtctgtg cagcgccaac ggcgtcatcg tgcccatgca
     2401 gtgcgagtac ttcgcgctcg aaggcctgac ggatctggtc aacaccatca agcaggtgca
     2461 cgccaacctg aaccgcgacc tgcagatcat tggcctgctg cgcgtcatgt tcgacccgcg
     2521 catcacgctg caaaaccagg tcagcgccca gctcaaggat cattttggcg acaaggtgtt
     2581 cgacaccgtc atcccgcgca acgtgcgcct ggccgaggcg cc
//
LOCUS       DAOMTQ010000263         2603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11688_length_2603_cov_20.031005, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000263 DAOMTQ010000000
VERSION     DAOMTQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11688_length_2603_cov_20.031005"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="PK518_15570"
     CDS             complement(<1..1861)
                     /locus_tag="PK518_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HRM50376.1"
                     /translation="MTMPLEIRLRGALRVLLSHYVANGVAGALGLLLISSLVHVLLGS
                     AAGSAAAVGAIVAIPPDIPAPRRGKFLHMLPAPLLALPLFFVVQLLHEQPWLLGLLIV
                     PASFFAFLAMAWGKRGAPVAIAAVLALVFSMAVPVHTGAEGWRAALTTSFYFGLGSLL
                     FVPWSVLVNRLLNGRYRAQVLADVLLSQAALMRLQALQFTPVLALGERANPLMGQLLL
                     RQATLADQLQSARDIVLESPGSARQQRLAGMLLCALEMRDHLLASALDLEELRRQPEQ
                     AGALTRQHGVLLALAAQMEQLADALLRGQVPAAFEDLRAQLQLAVPQDETGFTPARLA
                     QALSHRIGHIHDEVARLVALARGEAAPDLALVRVSWQMFVSPTGWGWRPLLGLWRWSA
                     APLRHAMRAALAIGVGYALSQLLPWREHAYWIVLTIVVVLRGSLAQTLERRNERVAGT
                     LLGCMLASIVLLANLPHWALLACVTLAQAVAHAFTLRRYLFTAVAATVLGLVQAHLLG
                     AGGSAGFVLLERMADTLIGAGIAWAFAYVLPSWERQQIPALVARALAAQTRHAREALA
                     LGQLQAVDNHPELAWRLARREAYDSLGVLVQATARAWKEPRAVRPPLAPLERMQ"
     gene            complement(1899..>2603)
                     /locus_tag="PK518_15575"
     CDS             complement(1899..>2603)
                     /locus_tag="PK518_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRM50377.1"
                     /translation="LLQIENEFYGTIRPKRTVQRGERPLHALRERGVEYVEVRLMDLD
                     PFENVGITAPTMRLLDIFLLHCLLSDSPPDTPQEIAEIKHNQHQTAERGREPGLQLLR
                     AGQAVPLADWAAQLLGECTPIATALDAAQGGGDYAAALQQAQARLADSSLTPSARVLA
                     AMRGQHGCSYEAFSFAQSQQAHERLLALPWDAAQQQHYEALAAQSLAAQKAIEDADTL
                     PFEDWRQQYMDPRQLG"
BASE COUNT          421 a          955 c          916 g          311 t
ORIGIN      
        1 cctgcatgcg ctccagcggc gccagcggcg ggcgcacggc gcgcggctcc ttccaggcgc
       61 gcgccgtggc ctgcaccagc acgcccaggc tgtcgtaggc ctcgcggcgc gccaggcgcc
      121 aggccagctc ggggtggttg tccacggcct gcagctggcc cagggccagg gcctcgcgcg
      181 catggcgcgt ctgcgccgcc agcgcgcgcg ccaccagagc cggtatctgc tggcgctccc
      241 aggagggcag cacataggca aaggcccagg cgatgccggc gcctatcagg gtgtcggcca
      301 tgcgctcaag cagcacgaag ccggcgctgc cgcccgcgcc cagcagatgc gcctgcacca
      361 gtccgagcac ggtggccgcc acggccgtga acaggtagcg gcgcagcgtg aaggcatggg
      421 ccacggcctg ggccagggtc acacaggcca gcagcgccca gtgcggcagg ttggccagca
      481 acacgatcga ggccagcatg cagcccagca gcgtgccggc cacgcgctcg ttgcgccgct
      541 ccagcgtctg cgccaggctg ccgcgcagca cgacgacgat ggtcagcacg atccagtagg
      601 catgctcgcg ccagggcagc agctgcgaca gcgcatagcc cacgccgatc gccagcgccg
      661 cgcgcatggc gtggcgcagc ggcgccgcgc tccagcgcca caggcccagc aatggccgcc
      721 agccccagcc cgtggggctg acgaacatct gccagctcac gcgcaccagg gccaggtcgg
      781 gcgcggcctc gccgcgcgcc agggcgacca ggcgcgccac ctcgtcatgg atatggccta
      841 tgcggtgcga cagcgcctgc gccagccgcg cgggggtaaa gccggtctcg tcctgcggca
      901 ccgccagctg cagttgcgcg cgcagatcct caaaagccgc cggcacctgc ccgcgcagca
      961 gggcatcggc cagctgctcc atctgcgcgg ccagcgccag cagcacgcca tgctggcgtg
     1021 tcagcgcccc ggcctgctcg ggctgccggc gcagttcctc cagatccagc gcgctggcca
     1081 gcagatggtc gcgcatctcc agcgcgcaca gcagcatgcc ggccagccgc tgctggcgcg
     1141 cgctgccggg cgactccagc acgatgtcgc gcgccgactg cagctgatcg gccagcgtcg
     1201 cctggcgcag caagagctgg cccatgagcg ggttggcgcg ctcgcccagg gccagcacgg
     1261 gcgtgaactg cagggcctgc agccgcatca gagcggcctg cgagagcagc acatcggcca
     1321 gcacctgcgc gcggtagcgg ccattgagca gccggttgac cagcaccgac cagggaacga
     1381 acagcagcga gcccaggcca aagtaaaagc tggtggtaag ggccgcgcgc cagccctcgg
     1441 ccccggtgtg caccggcacg gccatggaga acaccagcgc cagcacggcg gcgatggcca
     1501 cgggcgcgcc gcgcttgccc caggccatgg ccaggaaggc aaaaaaactc gccggcacga
     1561 tgagcagacc cagcaaccag ggctgctcgt gcagcagctg cacgacaaag aacagcggca
     1621 gcgccagcag cggcgcgggc agcatgtgca gaaacttgcc gcgccgcggc gctggaatgt
     1681 cgggcggaat ggcgacgatg gcccccaccg cggccgccga ccccgccgcg ctgcccagca
     1741 gcacatgcac cagcgaggaa atgagcagca gcccgagcgc accggccacg ccattggcga
     1801 cgtaatgcga cagcagcacc cgcagggcgc cgcgcagccg gatctcaagg ggcatggtca
     1861 tggcgcccca tcctacgacc gaatgggctc agccaggttc agccgagctg gcgcggatcc
     1921 atgtactgct ggcgccagtc ctcgaacggc agggtgtccg catcctcgat ggccttttgc
     1981 gccgccaggg actgcgcggc cagggcttcg tagtgctgct gctgcgccgc gtcccagggc
     2041 agggccagta gccgctcgtg cgcctgctgc gattgcgcaa aggaaaacgc ctcgtaactg
     2101 cagccatgtt ggccccgcat ggcggccagc acccgtgccg acggggtcag gctgctgtcg
     2161 gccagccggg cctgggcctg ctgcagcgcg gcggcgtagt cgccgccgcc ctgggcggca
     2221 tccagcgccg tggcgatggg ggtgcattcg cccagcagct gcgcggccca gtcggccagc
     2281 ggcaccgcct ggcccgcacg cagcagctgc aggcccggtt cgcgcccgcg ctccgcagtc
     2341 tggtgctggt tgtgcttgat ctcggcaatc tcctgcggcg tgtccggcgg actgtcggac
     2401 agcaggcagt gcagcaagaa tatgtccagc aggcgcatgg tgggggcggt gatgcccacg
     2461 ttctcgaacg gatccaggtc catcagccgc acctcgacat attccacgcc gcgctcgcgc
     2521 agcgcatgca gcgggcgctc gccgcgctgc acggtgcgct tggggcggat ggtgccgtag
     2581 aactcgttct cgatctgcag cag
//
LOCUS       DAOMTQ010000264         2582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11830_length_2582_cov_28.204986, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000264 DAOMTQ010000000
VERSION     DAOMTQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11830_length_2582_cov_28.204986"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PK518_15580"
     CDS             <1..1025
                     /locus_tag="PK518_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRM50378.1"
                     /translation="AAVPGTLTQSKSRWVPVAWSELPGFDADALHEAWNAWLRSCERP
                     APAFAPLCGDVRRLSIAAPEEQRGWIMQRLQPYRIETLEGSADGLLTGYFEPYLDAVR
                     QPGNGFGVPLYAPPADLGQRKPWYTRQQIETLPQAQAALAGRAIAWVRDPVEALVLHI
                     QGSGRLRVQEADGSQRLVRLAYAGTNDQPYKSVGRWLLDQGLVRDATWPGITAWVRQN
                     PQRVSEMLWSNPRYVFFREEPLDGLDAAFGPKGAQGVPLTPGRSIAVDRQSIPYGTPV
                     WLASSGPTAQLSRLVLAQDTGSAILGAVRADYFTGWGPEAGDMAGRLKQNLRLWALWP
                     RQGATP"
     gene            complement(1125..>2582)
                     /locus_tag="PK518_15585"
     CDS             complement(1125..>2582)
                     /locus_tag="PK518_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM50379.1"
                     /translation="MTTLTSSPVLVALVQTASTLPVFLLGLPSGALADILDRRKYFMV
                     TQFWVAAVAVVLCGVILAGGVSPHLLLALTFANGIGLAMRWPVFSAIVPELVERPHLP
                     AALALNGVAMNASRIIGPLLAGAIIASFGSAWVFVLNAVLSLLAGFTIMRWRRQATPN
                     PLGRERLASAMRVGLQFVRESPPMRAVLWRISVFFLHATALLALLPLVARGLQGGGAG
                     TFTLLLAAMGAGAIGAAMLLPRLRQMMAADQLVTRGTLVQSLASAIVALAPNVYLAVP
                     AMVVAGAAWITVANALVVAAQFALPNWVRARGMAIFQMAIMGATAAGAALWGQVAAMS
                     SVPVSLALAAAIGVTVMTLVQRLVSNRHGEEDLSPSRAFNLPQAETPPEAGLRLVTSI
                     EYLIEPARAAEFRAVMQESRRARLRQGALSWELQHDIADPRRYVERVVDESWTEHLRR
                     FDRVTASDVALRDRKNAFHIGETPPVISRYVVEGD"
BASE COUNT          400 a          906 c          928 g          348 t
ORIGIN      
        1 cggcggccgt gccgggcacg ctgacccagt ccaagagccg ctgggtgccc gtggcctgga
       61 gcgagctgcc cggctttgac gccgacgccc tgcacgaggc ctggaacgcc tggctgcgca
      121 gctgcgagcg cccggccccg gccttcgcgc cgctgtgtgg cgacgtgcgc cgcctgagca
      181 tcgccgcgcc cgaggagcag cgcggctgga tcatgcagcg cctgcagccc taccgcatag
      241 aaacgctgga gggcagcgcc gatggcctgc tgaccggcta cttcgagccc tacctggacg
      301 ccgtgcgcca gccgggcaac ggctttggcg taccgctgta tgccccgccg gcggatctgg
      361 gccagcgcaa gccctggtac acgcgccagc agatcgagac cctgccccag gcccaggcgg
      421 ccttggccgg ccgcgccatt gcatgggtgc gcgacccggt cgaggccctg gtgctgcaca
      481 tccagggctc gggccggctg cgcgtgcagg aggccgacgg cagccagcgc ctggtgcgcc
      541 tggcctatgc cggcacgaat gaccagccgt acaagagcgt gggccgctgg ctgctggacc
      601 agggtttggt gcgtgatgcc acctggcccg gcatcacggc ctgggtgcgg cagaacccgc
      661 agcgcgtcag cgagatgctg tggagcaacc cgcgctacgt gttctttcgc gaggagccat
      721 tggacgggct ggacgccgcc ttcggcccca agggcgcgca gggcgtgccg ctgacccccg
      781 gccgctccat cgccgtggac cgccagagca ttccctacgg cacgccggtg tggctggcct
      841 ccagcgggcc cacggcccag ctgtcgcgcc tggtgctggc gcaggacacg ggcagcgcca
      901 tcctgggcgc ggtgcgcgcc gactacttca ccggctgggg cccggaggcg ggcgacatgg
      961 ccgggcggct gaagcagaac ctgcgcctgt gggcgttgtg gccgaggcag ggcgccaccc
     1021 cctgaggcgc ccgtatttga gcgcgcagct tgcggtggat attggcaaaa atagactgta
     1081 gcgtttattg ataaagcgtc aatagctatt aattgtatag cttatcaatc gccttccacc
     1141 acatagcgcg aaattaccgg cggtgtctcg ccgatatgga aggcgttctt gcggtcgcgc
     1201 agtgccacgt cggaggcggt gacgcggtcg aagcggcgca ggtgttcggt ccaggactcg
     1261 tccaccacgc gctcgacgta gcggcgcggg tcggcgatgt cgtgctgcag ctcccagctg
     1321 agggcaccct ggcgcaggcg cgcgcgccgg ctctcctgca tcacggcgcg aaactcggcc
     1381 gcgcgcgcgg gctcgatgag gtattcgatg ctggtcacca ggcgcagccc ggcctctggc
     1441 ggggtctcgg cctgcggcag gttgaaggcg cgcgaggggc tcaggtcttc ctcgccatgg
     1501 cggttgctca ccaggcgctg caccagggtc attaccgtca cgcctatggc cgcagccagc
     1561 gccaggctca cgggcacgct gctcatggcg gccacctggc cccacagcgc cgcgccggct
     1621 gcggtggcgc ccatgatggc catctggaag atggccatgc cgcgcgcgcg cacccagttg
     1681 ggcagggcga actgtgccgc caccaccagg gcgttggcca cggtgatcca ggccgcaccg
     1741 gcgaccacca tggccggcac ggccagatac acgttgggtg ccagggccac gatggccgag
     1801 gccagcgact gcaccagcgt gccgcgcgtg accagctggt cggcggccat catctggcgc
     1861 aggcgtggca gcagcatggc cgcgcctatg gcacccgcgc ccatggccgc cagcagcagc
     1921 gtgaaggtgc cggcgccgcc gccctgcagg ccgcgtgcca ccagcggcag cagcgccagc
     1981 agggccgtgg cgtgcaggaa gaacaccgaa atgcgccaca gcacggcgcg catcggtggc
     2041 gactcgcgta cgaactgcag gcccacgcgc atggcgctgg ccaggcgctc gcgcccgagc
     2101 gggttgggcg tggcctggcg ccgccagcgc atgatggtga agcccgccag cagcgacagc
     2161 acggcgttca gcacaaagac ccaggcgctg ccgaagctgg caatgatggc gccggccagc
     2221 aggggcccga tgatgcgcga ggcgttcatc gccacgccgt tgagcgccag cgccgcgggc
     2281 aggtgcgggc gctccactag ctcgggcacg atggccgaga acaccggcca gcgcatggcc
     2341 aggcctatgc cgttggcaaa ggtgagcgcc agcagcaggt gcgggctgac cccgccggcc
     2401 aggatcaccc cgcacagcac cacggccacg gccgccaccc agaactgcgt gaccatgaag
     2461 tacttgcgcc ggtccagtat gtcggccagc gcaccgctgg gcaggccgag caggaacacc
     2521 ggcagggtgg aggccgtctg caccagcgcc accagcaccg gcgagctggt cagcgtggtc
     2581 at
//
LOCUS       DAOMTQ010000265         2567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11944_length_2567_cov_27.700637, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000265 DAOMTQ010000000
VERSION     DAOMTQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11944_length_2567_cov_27.700637"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PK518_15590"
     CDS             complement(<1..553)
                     /locus_tag="PK518_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50380.1"
                     /translation="MSLFSWFTRKSKPQAPAAAAAAPGAHGKPLPSSPSAPSQDQVRR
                     SERTERRELLYTAVRDAMVRAGVLSAGYKFKVLSLDQRGSQFLVMMDLAPEYGGDMGR
                     LGEIETLIAQSAKTRFGIGVPAVYWRTNAQIVVGAALRQAVAGAPAQSRVPEFTAGAV
                     AASPVVVAPRPAAARQAAPRPTAL"
     gene            complement(635..1126)
                     /locus_tag="PK518_15595"
     CDS             complement(635..1126)
                     /locus_tag="PK518_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRM50381.1"
                     /translation="MNIAAIPILKAQLMGLVQAISQAATEDSMGNMLSSEQWDLLLPY
                     LRPMELPVGQVLFAQGTTERTLYLVESGSLTVHYEDEQQRVRLAIVGPGSVVGEGAFF
                     SHRPRSATVQAGAPTRLWNLTAIRFAEFANRNPAVALQLVMAIGAVQAKRLANRRRRV
                     AST"
     gene            complement(1197..>2567)
                     /gene="mnmE"
                     /locus_tag="PK518_15600"
     CDS             complement(1197..>2567)
                     /gene="mnmE"
                     /locus_tag="PK518_15600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRM50382.1"
                     /translation="AAIATAPGRGAVGIVRVSGRGLAPFVQDLLGRALQPRQAHYLPF
                     PDAQGQPIDQGLALFFPAPHSYTGEDVLELQAHGGPVVLQLLLARCLEAAQTALPRLR
                     LAEPGEFTERAFLNDKIDLAQAEAIADLIDASTEAAARSAGRSLSGEFSKEIHGLRDA
                     LIHLRMLVEATLDFPEEEIDFLRKADARGQLSNLQQTLAGVMQRAGQGALLREGIKVV
                     IAGQPNAGKSSLLNALAGAELAIVTPIAGTTRDKVQQTIQIEGVPLHIIDTAGLRESS
                     DEVERIGIARAWEEIAGADAVLFLHDLTRMNAPEYIAGDAAITSDLDQKLPENVPVID
                     VWNKLDASTHQPPQAVAGHEAVRLSARTGAGLDTLRQRLLQVAGWQQGAEGVYIARAR
                     HLEALRAVDAHLMEAAAQLMSDGPALDLLAEELRLAQNALNSITGEFGADDLLGVIFS
                     SFCIGK"
BASE COUNT          400 a          877 c          883 g          407 t
ORIGIN      
        1 ccaatgcggt cgggcgtggg gcggcctggc gcgctgccgc gggccgcgga gcgaccacga
       61 ccggcgaggc cgcaacggcc cctgccgtga attcgggcac gcggctctgc gctggcgcac
      121 ctgccacggc ctggcgcagc gcggcgccca ccacgatctg cgcattagtg cgccaataca
      181 cggccggcac gccgatacca aagcgcgtct tggcggactg ggcgatcagc gtctcgatct
      241 cgcccagacg acccatgtcg ccgccatatt cgggcgccag atccatcatc accaggaact
      301 gcgagccccg ctgatccagc gacagcacct tgaacttgta gcccgccgac agcacgccgg
      361 cacgcaccat ggcgtcgcgc accgcggtgt agagcagctc gcggcgctcg gtgcgctcgc
      421 tcctgcgcac ctgatcctgc gagggcgccg acggcgagga tggcaacggc ttgccatggg
      481 caccgggtgc cgcagcggct gcggccggtg cctggggctt ggatttgcga gtgaaccaac
      541 tgaacaatga catacctgcc ttcgcgaaga agtgctaaat ttgtcagtaa gtgtactgcg
      601 caccagcatc aacgccacgc cgggcgtggg actgtcaggt ggatgcaacg cggcggcggc
      661 ggttggccag gcgcttggcc tgcacggcgc ctatggccat gaccagctgc agggccacgg
      721 ccgggttgcg gttggcgaac tccgcgaagc ggatcgccgt caggttccac agcctggtcg
      781 gcgccccggc ctgcaccgtg gcgctgcgcg ggcggtgcga gaaaaacgcg ccctcgccca
      841 ccaccgagcc cgggccgacg atggccaggc gcacgcgctg ctgctcgtcc tcatagtgca
      901 cggtcaggct gccgctttcg accaggtaca gggtgcgttc ggtcgtgccc tgggcaaaca
      961 acacctggcc gaccggcagc tccatgggcc gcagataggg caggagcaga tcccactgct
     1021 cggagctgag catattgccc atgctgtctt cggtggcggc ctggctgatg gcctgcacca
     1081 ggcccatcag ctgggccttg aggatgggaa tggcggcgat gttcatcgcg cgcgaaagta
     1141 acgtggtcgt gccgttcaca caagcacgca ttacttttgt tcacaaaaac gtcgcttcac
     1201 ttgccgatgc agaagctgga aaaaatcacc cccagcaggt cgtccgcgcc aaattcgccg
     1261 gtgatgctgt tgagcgcgtt ctgcgccagg cgcagctcct cggccagcag atccagcgcg
     1321 gggccatcgc tcatcagctg cgccgccgcc tccatcaggt gtgcgtccac ggcgcgcagg
     1381 gcctcgaggt ggcgcgcgcg cgcgatgtac acaccttcgg cgccctgctg ccagccggcg
     1441 acctgcagca gccgctggcg caacgtgtcc agcccggcgc cggtgcgcgc cgacagccgc
     1501 acggcctcgt ggccggcgac cgcctggggc ggttggtgcg tactggcatc gagcttgttc
     1561 cagacgtcga tcaccggcac attttcgggc agtttttggt ccagatcgct tgtgatggca
     1621 gcgtcaccag ctatgtattc gggagcattc atgcgcgtca agtcgtgcag gaacaacacg
     1681 gcgtcggcgc ccgcgatctc ctcccaggca cgggcgatgc ctatgcgctc gacctcgtcg
     1741 ctgctctcgc gcaggccggc ggtgtcgatg atgtgcagcg gcacgccctc gatctgaatg
     1801 gtctgctgca ccttgtcgcg cgtggtgccg gcgatcgggg tgacgatggc cagctcggcg
     1861 cccgccaggg cgttgagcag cgagctcttg ccggcgttgg gctggccggc gatgaccacc
     1921 ttgatgccct cgcgcagcaa cgcgccctgc cccgcgcgct gcatcacgcc ggccagggtt
     1981 tgctgcaaat ttgagagctg tccgcgcgcg tccgccttgc gcaggaagtc gatttcctcc
     2041 tcgggaaagt ccagcgtggc ctcgaccagc atgcgcaggt ggatgagggc gtcgcgcagg
     2101 ccgtggatct ctttggagaa ctcgcccgag agcgagcgcc cggcgctgcg cgctgcagcc
     2161 tcggtgctgg cgtctatcag gtcggcaatc gcctcagcct gggccaggtc gatcttgtcg
     2221 ttcaagaacg cccgctcggt gaactcgccg ggctcggcca ggcgcaggcg cggcagagcg
     2281 gtctgcgccg cctccaggca gcgcgccagc agcagctgca gcaccacggg cccgccgtgg
     2341 gcctggagct ccagcacgtc ctcaccggtg tagctgtgcg gcgcggggaa gaaaagcgcc
     2401 agcccttggt cgatgggctg gccctgggcg tcgggaaagg gcaggtaatg cgcctggcgc
     2461 ggctgcagcg cgcggcccag cagatcctgc acgaagggcg ccaggccacg gcccgacacg
     2521 cgcacgatgc ccacggcgcc acgccctggg gcggtggcga tggcggc
//
LOCUS       DAOMTQ010000266         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_11963_length_2564_cov_19.260662, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000266 DAOMTQ010000000
VERSION     DAOMTQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11963_length_2564_cov_19.260662"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK518_15605"
     CDS             complement(<1..200)
                     /locus_tag="PK518_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50383.1"
                     /translation="MKKALIWLHKWLGLVLALFFLMWFASGVVLYFVPFPSLTQTERL
                     QAAPPLALPAGCCLSAAAAARQA"
     gene            complement(203..1654)
                     /locus_tag="PK518_15610"
     CDS             complement(203..1654)
                     /locus_tag="PK518_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRM50384.1"
                     /translation="MDVQRHNYALALTARASSALSATLSLDGGVNDDARYFGTPLRNG
                     QLDARLRRSNYNVADSVVRYDDRMWRLKVDYQVNAALRLRNETYHLSTDRHWRNAESY
                     AFNAAGTQILRGDYLEILHDQQQTGNRLDATLDGQLGGLKNRFIVGLDWYRSHLVQGS
                     NAPYGGASSVDPFNFDPGLFTSPVPTRSTRDATLRTTAWFAENALDLSRQWKLVAGLR
                     SERMALAQVNLTNGASLGKQYQPLTGRLGAVWSPGSALSLYGQWASGTDPLSGALSLP
                     GGGDKFDLTRGRQVEVGAKGALPALRGEWTLALYQIKKRNLLSRDASNPAITQQIGQQ
                     SSTGLELAVAAEPLRGWTIDANAALLRARYDDYQELVAGKLVSRSGNTPTGVPERAFN
                     VWSAWRLAPQWRLGLGLRHVGARQGNTANTTQLPAYTVWDAGLGYEPTRQLQVSLSVK
                     NLANRDYAVSGQAGKSWLLGAPRTVMLTLRTQF"
     gene            complement(1745..2341)
                     /locus_tag="PK518_15615"
     CDS             complement(1745..2341)
                     /locus_tag="PK518_15615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRM50385.1"
                     /translation="MRNFCYQPRHLPLHSLALAVSGVAFSVQAQESATLPAVQVRAAP
                     TPAGSSLGLDEPSATGSRLGLTARETPASVSSLGAADMAERDLTRAQDAAVRMPGITE
                     SPSPGNGGTALVARGFGGHNSVAQMVDGTRLIVASGSISYPFSSWPLESVQVLRGPAS
                     VLYGDGAIGAAVNYVTKQPRFDKTEREAFVAAGSYGAL"
     gene            complement(2373..>2564)
                     /locus_tag="PK518_15620"
     CDS             complement(2373..>2564)
                     /locus_tag="PK518_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRM50386.1"
                     /translation="AQEVRRGLAGACLWLLTDGRTLESPRAPQGAQLVLVDFDDRLRP
                     AGRCPAWARDWRADYRLPG"
BASE COUNT          384 a          881 c          921 g          378 t
ORIGIN      
        1 gcctggcgcg ccgccgccgc cgcgctcagg cagcagcccg caggcagcgc cagcggcggc
       61 gcggcctgca ggcgctcggt ctgcgtcagg ctgggaaagg gcacgaagta cagcaccacg
      121 ccgctggcaa accacatcaa aaaaaacaac gccagcacca ggcccagcca tttgtgcagc
      181 cagatcagcg cctttttcat actcagaatt gcgtgcgcag cgtcagcatc acggtgcgtg
      241 gcgcgcccag cagccagctc ttgccggcct ggccggagac ggcgtagtcg cggttggcca
      301 ggttcttcac cgacagactc acttgcagct ggcgcgtggg ctcgtagccc aggccggcgt
      361 cccacacggt gtaggcgggc agctgggtgg tgttggcggt gttgccctgg cgcgcgccca
      421 catggcgcag gcccaggccc aggcgccact gcggcgccag gcgccaggcg ctccagacgt
      481 tgaacgcgcg ctcgggcacg cccgtcggcg tattgccgct gcgcgagacc agcttgcccg
      541 ccaccagctc ctggtagtcg tcgtagcgcg cgcgcagcaa ggcggcgttg gcgtctatcg
      601 tccagccgcg cagcggctcg gcagccacgg ccagctccag gccggtggag gactgctggc
      661 cgatctgctg cgtaatggcc gggttgctgg cgtcgcgcga cagcaggttg cgctttttga
      721 tctggtacag cgccagcgtc cattcgccgc gcagcgcggg cagcgcgccc ttggcgccga
      781 cttcgacctg gcggccccgg gtcaaatcga acttgtcgcc gccgccgggc agcgacagcg
      841 cgcccgacag cgggtcggtg ccgctggccc attgaccgta cagcgacagc gcgctgccgg
      901 gcgaccagac cgcgcccagg cggccggtga gcggctggta ttgcttgccc aggctggcgc
      961 cattcgtcag gttgacctgc gccagcgcca tgcgctcgct gcgcaggccg gccaccagct
     1021 tccactggcg cgacaggtcg agcgcgttct cggcaaacca ggccgttgtg cgcagcgtgg
     1081 cgtcgcgcgt cgaacgggtg ggcacggggc tggtgaacag gccggggtcg aagttgaacg
     1141 gatcgacgct gctggcgccg ccgtagggcg cattgctgcc ctgcaccaaa tggctgcggt
     1201 accagtccag gccgacgatg aagcgatttt tcaggccgcc gagctggcca tcgagggtgg
     1261 cgtccaggcg gttgccggtc tgctgctggt cgtgcaggat ttccaggtag tcgccgcgca
     1321 aaatctgcgt gccggcggcg ttgaaggcgt agctctcggc gttgcgccag tggcggtcgg
     1381 tgctcaggtg gtaggtctcg ttgcgcaggc gcagcgcggc attcacctgg tagtccacct
     1441 tcaggcgcca catgcggtcg tcgtagcgca ccaccgagtc ggccacgttg tagttgctgc
     1501 ggcgcaggcg cgcatccaac tggccattgc gcagcggcgt gccgaagtag cgcgcgtcgt
     1561 cgttcacgcc gccgtccagc gacagcgtgg ccgacagcgc gctgctggcg cgcgccgtca
     1621 gcgccagggc gtagttgtgg cgctgcacgt ccatgaagcg gcggtagccg tcgctttttt
     1681 ctgcatccag atagaccgag taggccaggc tgtcattgat gggcccacgc aggccgacgc
     1741 cgccctacag cgcgccgtag gagccggcgg cgacgaaggc ctcgcgctcg gtcttgtcaa
     1801 agcgcggctg cttggtgacg tagttcaccg ccgcgccgat ggcgccgtcg ccgtagagca
     1861 ccgaggccgg gccgcgcagc acctgcaccg actccagcgg ccagctcgaa aacgggtagc
     1921 tgatgctgcc cgaggcaacg atcaggcgcg tgccatcgac catctgcgcc accgagttgt
     1981 ggccgccaaa gccgcgcgcc accagggccg tgccgccgtt gccggggctg ggcgactctg
     2041 ttatgccggg catgcgcacc gccgcgtcct gggcgcgggt caggtcacgc tcggccatgt
     2101 cggccgcccc caggctgctg acgctggccg gcgtctcgcg cgccgtcagg cccaggcgcg
     2161 aaccggtggc cgagggctca tcgagcccca ggctggagcc ggccggggtg ggcgcggcgc
     2221 gcacctgcac ggcgggcaag gtggcgcttt cctgggcctg gacagaaaag gccacgccag
     2281 aaacggccag ggccagggaa tgcaacggca ggtgccgggg ctgatagcag aagttgcgca
     2341 tgaagatttt ttcttgaaaa cacagagaaa cgtcagccgg gcaggcggta atccgccctc
     2401 cagtcccgcg cccaggccgg gcagcggccg gccgggcgca ggcggtcgtc aaagtccacc
     2461 agcaccagct gcgcgccctg cggcgcgcgc ggcgattcga gcgtgcggcc atcggtcagc
     2521 agccacaggc aggcgccggc caggccgcgc cgaacctcct gggc
//
LOCUS       DAOMTQ010000267         2558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_12012_length_2558_cov_21.995605, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000267 DAOMTQ010000000
VERSION     DAOMTQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12012_length_2558_cov_21.995605"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK518_15625"
     CDS             complement(<1..675)
                     /locus_tag="PK518_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM50387.1"
                     /translation="MSLLRVEGLRKSFGGVQAVQGVSFALAAGELLALIGPNGAGKST
                     CFNMLGGQIRPDAGRVLLAGQDITGLPPRVIWRLGVGRTFQIAQTCASFTVLQNLQLA
                     LLSHDRRGWRWWRRAGQHRVPDALALLEQVGMQDQADRPCSELAYGDVKRVELAMALA
                     HAPRLLLMDEPTAGMASAERLALMRLVRRLARERAMAVLFTEHSMDVVFGQADRVAVL
                     VRGQLLA"
     gene            complement(672..>2558)
                     /locus_tag="PK518_15630"
     CDS             complement(672..>2558)
                     /locus_tag="PK518_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM50388.1"
                     /translation="SGLLAQLLNGLAGASTLFLVAAGLSLIFGVTRIVNFAHGSFYML
                     GLYVAYSSVALLGDAIGFWPALLLAPLVLGLLGALVELLLLRRVYGAPELLQLLATFA
                     LVLVIKDAALWLWGPQELFGPRAPGLEGAVDILGRRFPAYDLLLIAVGPLVLLLLTLL
                     LTRTRWGTLVRAATQDREMVGALGVNQAWLFTGVFALGAALAGLGGALQLPREPASLE
                     LDMLTIGAAFVVVVVGGMGSLPGAFVAALLIAELKAVCIWLGVVQIAGVAIAFPKLTL
                     VVEFLVMAAVLIWRPWGLMGRPQAAVRGVGAPEAPLAGAGPLAGTGWAVLLLALALLP
                     LALGQGSYASVLLADIFIAALFAASLHAIVGPGGLHSFGHAAYFGLGAYGAALLTRAA
                     GLPMGAALLLAPLVAAGGALLYGWFCVRLSGVSLTMLTLAFAQITWAIAFQWDGLTGG
                     SNGLTGVWPPDWAQGTAFYWLVLAFVAAGLYLLRRLLFAPLGWALRAARDSATRAEAI
                     GINVRGVQWAAFVVAGLLAGLAGALFTFSKGGVAPEVLWVGKSVDGLVMVLLGGMQQL
                     AGPLVGAAAFTWLHDSVARATDYWRALLGGIMLALVLLFPQGISGFAQYWQALIAPKK
                     GVIA"
BASE COUNT          469 a          969 c          871 g          249 t
ORIGIN      
        1 ggccagcagc tggccgcgca ccagcacggc cacgcggtcg gcctggccga agaccacgtc
       61 catgctgtgc tcggtgaaca gcacagccat ggcgcgctcg cgcgcgaggc gccgcaccag
      121 ccgcatcagc gccaggcgct cggcgctggc catgccggcc gtgggctcgt ccatcaacag
      181 caggcgcggc gcgtgcgcca gggccatggc cagctcgacg cgcttgacat cgccataggc
      241 cagctcgctg caggggcggt cggcctgatc ctgcatgccc acctgctcca gcagggccag
      301 cgcgtccggc acgcggtgct ggccggcgcg gcgccaccag cgccagccgc gtcggtcgtg
      361 cgacagcagg gccagctgca ggttctgcag cacggtgaag ctggcgcagg tctgggcaat
      421 ctggaaggta cggcccacac ccaggcgcca gatgacgcgc ggcggcaggc cggtgatgtc
      481 ctggccggcc agcaacacgc ggccggcgtc gggccggatc tgcccgccca gcatgttgaa
      541 gcaggtgctc ttgcccgcgc cgttggggcc gatcagcgcc agcagctcgc cggccgccag
      601 cgcgaacgac acgccctgca cggcctgcac gccgccaaag gacttgcgca ggccctccac
      661 gcgcagcagg ctcatgcgat tactcctttt ttaggagcta tcagcgcttg ccaatattgc
      721 gcaaaccccg aaattccctg tggaaacagc agcaccagcg ccagcatgat gccgcccagc
      781 agcgcgcgcc agtagtcggt ggcgcgggcc acgctgtcgt gcagccaggt gaaggccgcc
      841 gcgcccacca gcgggccggc cagctgctgc atgccgccca gcagcaccat caccaggccg
      901 tcgaccgact tgccgaccca cagcacctcg ggcgccacgc cgccctttga aaaagtgaac
      961 agcgcgcccg ccagccccgc cagcaggccg gcgacgacga aggccgccca ttgcacgccg
     1021 cgcacgttga tgccaatggc ctcggcgcgc gtggccgagt cgcgcgcggc gcgcagcgcc
     1081 cagcccaggg gcgcgaacag caggcgccgc agcaggtaga ggcccgccgc gacaaaagcc
     1141 agcaccagcc agtagaaggc cgtgccctgc gcccaatcgg gcggccaaac gccggtcagg
     1201 ccattgctgc cgcccgtgag gccgtcccac tggaaggcga tggcccaggt gatttgcgcg
     1261 aaggccagcg tcagcatggt cagcgacacg cccgacaggc gcacgcaaaa ccagccatag
     1321 agcagcgccc cgcccgccgc caccaggggg gccagcagca gcgccgcgcc catgggcagg
     1381 cccgccgcgc gcgtgagcag cgccgcgcca taggcgccca ggccaaagta ggccgcgtgg
     1441 ccaaacgaat gcagcccgcc cggcccgacg atggcgtgca ggctggcggc aaacagcgcg
     1501 gcgatgaaga tgtcggccag cagcacgctg gcgtagctgc cctggcccag agcgagcggc
     1561 agcagcgcca atgccagcag caggacggcc cagccggtgc cggccagcgg gccggcgccg
     1621 gccaggggcg cctcgggcgc gcccacgccg cgcacggcgg cctgcggccg gcccatcaac
     1681 ccccaggggc gccagatgag cacggcagcc atgaccagaa actccaccac cagggtgagt
     1741 ttggggaagg cgatcgccac ccctgcgatc tgcaccacgc ccagccagat gcacacggcc
     1801 ttgagctcgg cgatcagcag cgcagcgaca aaggcgccgg gcagcgagcc catgccgccc
     1861 accaccacga cgacgaaggc cgcgcctatg gtcagcatgt ccagttccag gctggcgggc
     1921 tcgcgcggca gctgcagcgc gccacccagg ccggccagcg ccgcgcccag cgcgaacacg
     1981 ccggtgaaca gccaggcctg gttcacgccc agggcgccga ccatctcgcg gtcctgcgtg
     2041 gcggcgcgca ccagcgtgcc ccagcgcgtg cgcgtcagca acagcgtgag cagcagcagc
     2101 accagcgggc cgacggcgat gagcagcagg tcataggccg gaaagcgccg gcccaggatg
     2161 tccaccgcgc cttccagccc cggcgcgcgc gggccgaaca gttcctgcgg gccccacagc
     2221 cacagcgccg cgtccttgat caccagcacc agggcaaagg tggccagcag ctgcagcagc
     2281 tctggcgcac cgtagacacg gcgcaggagc agcagctcga ccagcgcgcc caagaggccc
     2341 agaaccagcg gcgccagcag cagcgcgggc cagaagccta tcgcatcgcc cagcagcgcg
     2401 acgctggaat acgctacgta cagccccagc atgtagaacg agccatgggc aaagttgacg
     2461 atgcgcgtga cgccaaagat cagcgacagc ccggccgcca ccaggaacag cgtggacgcg
     2521 ccggccaggc cgttgagcag ctgggccagc aggccgga
//
LOCUS       DAOMTQ010000268         2515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_12329_length_2515_cov_25.497561, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000268 DAOMTQ010000000
VERSION     DAOMTQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12329_length_2515_cov_25.497561"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /gene="accB"
                     /locus_tag="PK518_15635"
     CDS             complement(<1..388)
                     /gene="accB"
                     /locus_tag="PK518_15635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRM50389.1"
                     /translation="MDLRKLKTLIDLVSESNVSELEITEAEGKVRIVKSGTAVVQQFV
                     AAPVQQAPVAAAAAAVAAPVAELPAPAAAAMPAGHTVKSPMVGTFYRASSPGAKPFVE
                     VGSQVKEGETICIIEAMKILNEIEADK"
     gene            complement(496..1068)
                     /locus_tag="PK518_15640"
     CDS             complement(496..1068)
                     /locus_tag="PK518_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRM50390.1"
                     /translation="MSHSSVEPGVDSSQALQASRRRLFLWGAGAAAGLAGAGLAAWRL
                     RVADAQADQPQALWDASFEGVGGETLQMASMRGRPLLLNFWATWCPPCVQELPLLNAF
                     YGEHRAKGWQVLGLAIDQPANVQKFLQRMPLDFPVVLGGLGGAELSRGLGNDKGGLPF
                     TVMFAADGSISKRKIGQLTEKDLESWFKSV"
     gene            complement(1065..1676)
                     /locus_tag="PK518_15645"
     CDS             complement(1065..1676)
                     /locus_tag="PK518_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50391.1"
                     /translation="MENALATEIANSAARLVVEEGLDWGPAKRRAVKVLGLPARTPLP
                     DNEQVEDAVRAYIELFCADTQPRELRALRELALLWMERMQAFRPYLGGAVWHGTATRL
                     SDIFIALFCDDPKSAEIALIDHRVNYEPGSTKGMRGEQVEVLSLSSHSRELNEEIGVH
                     LLIYDLDDLRGALRPDGRGRTPRGDIDAVRRLLQQSPTSLVHP"
     gene            1884..>2515
                     /locus_tag="PK518_15650"
     CDS             1884..>2515
                     /locus_tag="PK518_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRM50392.1"
                     /translation="MHIHILGICGTFMGGLAALAREAGHRVTGCDSGVYPPMSDQLRA
                     LGIELIEGYGAEQLALAPDMFVVGNVVSRARLADGSPKFPLMEAILDAGAPYTSGPQW
                     LAEQVLTGRHVLAVAGTHGKTTTTSMLAWILECAGLAPGFLVGGVPMNFGISARLGSL
                     AAGQERPLFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDL"
BASE COUNT          475 a          840 c          768 g          432 t
ORIGIN      
        1 acttgtcggc ctcgatctcg ttgaggatct tcatggcctc gatgatgcag atggtttcgc
       61 cttccttgac ctggctgcct acctcgacaa aaggcttggc accagggctg gaggcacggt
      121 agaacgtgcc taccatgggc gacttgaccg tgtggcccgc gggcatggcc gcagcagccg
      181 gggccggcaa ttcggccacg ggcgccgcga cagctgccgc ggcggcagcc accggcgcct
      241 gttgcacggg agcggcgaca aactgctgca ccacggccgt accgctcttg acgatacgca
      301 ccttgccctc ggcttcggtg atctcgagtt cggaaacatt cgactcggag accagatcaa
      361 tgagggtttt gagttttcgc aaatccatgg gagctccaac ggctagaaac ggaatggggg
      421 gcgaatttac cctaatttcg gtctatttca ccgaaacaac cccaaaaatc cactttctca
      481 tgcagaggac cggggttaca cgctcttgaa ccagctttcc agatctttct ccgtcaactg
      541 gcctatttta cgcttcgata tgctgccatc tgcggcaaac atcaccgtaa atggcagccc
      601 tcccttgtcg ttgcccaagc cacgcgacag ctcggctccg ccgaggccgc ccaacaccac
      661 gggaaagtcc agcggcatgc gttgcaggaa tttttgcaca ttggccggct gatcgatagc
      721 caggccaagc acctgccagc ctttggcgcg gtgttcgccg taaaaggcgt tcagcagggg
      781 cagctcctgc acgcagggcg ggcaccaggt ggcccagaag ttcagcagca aggggcggcc
      841 acgcatggag gccatctgca acgtctcgcc gccaaccccc tcgaagctgg cgtcccacag
      901 ggcctgcggc tgatccgcct gcgcgtcggc gacccgcaag cgccacgcgg ccaggcccgc
      961 cccggccagg ccggccgccg cgccggcccc ccacaggaac aagcgacgac gcgaggcctg
     1021 cagcgcctgc gatgaatcaa caccaggttc gacggatgaa tggctcatgg atgcaccaag
     1081 gaagtagggg attgctgcag caaccggcga acagcatcga tgtcgccccg cggcgtgcgg
     1141 ccacgcccat cgggacgcaa cgcgccgcgc agatcgtcca ggtcatagat caacaagtgc
     1201 acaccgatct cctcgttcag ctcgcggctg tggctgctca ggctgagcac ctcgacctgc
     1261 tcgccgcgca tgcccttggt gctgcccggc tcatagttca cacggtggtc tatcagcgcg
     1321 atttccgcag acttgggatc gtcgcagaac agcgcaatga aaatgtcgga caggcgcgtg
     1381 gccgtgccat gccagaccgc gccgcccaga taggggcgaa aggcctgcat gcgctccatc
     1441 cacaacaggg ccagctcgcg cagagcgcgc aactcgcgcg gctgggtgtc ggcacagaac
     1501 agctctatgt aggcgcgcac cgcatcctcg acctgctcgt tgtcgggcaa gggcgtgcgc
     1561 gcgggcaggc ccagcacctt gacggcgcgg cgcttggccg gcccccagtc caacccctcc
     1621 tcgaccacca ggcgggctgc ggagttggca atttcggtag cgagggcgtt ttccatggtg
     1681 gcgacaatcc cggacacggg cctccattgt ggcgtcaagc ccaggccaag acagggaata
     1741 ccccgtgcac gacacaccgg gtttgctgac acctccaaag aatactccta attcgatagc
     1801 tatttgcgct gattgcacaa gcatcaatgg ccatttttgt tcaaaatcca taaccagacg
     1861 ccagctccct agaatcacgc cccatgcaca tacatatcct aggcatttgc ggcactttca
     1921 tgggcggcct ggccgccctg gcgcgcgagg cgggtcaccg cgtcaccggc tgcgacagcg
     1981 gcgtctaccc gccgatgagc gaccagctgc gcgcgctggg catagagctg atcgagggct
     2041 atggcgccga gcagctggcg ctggcgcccg acatgttcgt cgtcggcaac gtggtcagcc
     2101 gcgcccggct ggccgatggc agcccgaaat tcccgctgat ggaggccatt ctggacgcgg
     2161 gcgcgcccta taccagcggc ccgcaatggc tggccgagca ggtgctgacc ggccgccatg
     2221 tgctggccgt ggccggcacg catggcaaga ccaccaccac ctcgatgctg gcctggattc
     2281 tggaatgcgc gggcctggcg cccggctttc tggtcggcgg cgtgcccatg aacttcggca
     2341 tctcggcgcg cctggggtcg ctggcggcag gacaggagcg gccactgttc gtgattgagg
     2401 ccgacgagta cgacaccgcc ttcttcgaca aacgcagcaa gttcgtgcac taccgcccgc
     2461 gcacggccgt gctgaacaac ctggagttcg atcacgccga catctttgac gatct
//
LOCUS       DAOMTQ010000269         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_12350_length_2511_cov_26.552932, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000269 DAOMTQ010000000
VERSION     DAOMTQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12350_length_2511_cov_26.552932"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PK518_15655"
     CDS             <1..1584
                     /locus_tag="PK518_15655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM50393.1"
                     /translation="AGEQLKLLNGNTVTLDEKVGVIADERAVESLAGIMGGDATAVSD
                     DTRHIYVEAAFWWPEAVAGRSRRYNFSTDAGHRFERGVDPQLTVEHIERITQLIVEIC
                     GTPETACAPIDDQQVNLPAPNPVRMRVARAAKVIGMPLTLAQCVDALKRLGLPVQQGE
                     GVLTVQPPSFRFDLRLEEDLIEEVARVVGYNNLPTTPPLAPITPKLRLESHRSSFAVR
                     RELAALGYQETINFSFVEEHWEKELAGNDTPIKLLNPIASQLSVMRSSLLGSLLQVLK
                     FNLDRKAARVRVFELGRVFLRDASVKNTDTTVEGFHQPMRVAGLAYGAPDALQWGRKD
                     KAVDFYDAKGDVEALLAPLRPTFESATHPALHPGRCARVLLAGQAIGYVGELHPQWRQ
                     SWDLPTAPMLFELELDAVLQRQVPAFRAVAKRQLVERDIAVVVAERVTHDEVMRAIRG
                     SLPGELLRSAVLFDVFRPQPQRAGEAMAAGAIAQGEKSLAIRLTLGSDAEALTDAQID
                     AAVQSVLQALTQQTAARLR"
     gene            1602..1919
                     /locus_tag="PK518_15660"
     CDS             1602..1919
                     /locus_tag="PK518_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRM50394.1"
                     /translation="MAIEFAVDSLESPALTKAQLADLLFEHIGLNKRESKDMVDAFFD
                     LIAQSLIAGEDVKLSGFGNFQIRTKAARPGRNPRTGEAIPIEARRVVTFHASSKLKEQ
                     IQG"
     gene            2031..2432
                     /locus_tag="PK518_15665"
     CDS             2031..2432
                     /locus_tag="PK518_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM50395.1"
                     /translation="MSQSLPAIPVKRYFTIGEVAQLCGVKPHVLRYWEQEFTQLRPMK
                     RQGNRRYYQHHEVLMIRRIRDLLYDQGFTIVGARNRLHELAQADKGARDAALPPDALD
                     DQPIDRAPALALPLDSNALRTELEEIRALLF"
BASE COUNT          437 a          813 c          813 g          448 t
ORIGIN      
        1 gcgggcgagc agctcaagct gctgaacggc aacaccgtca cgctggacga aaaggtcggc
       61 gtgatcgccg acgagcgtgc agtcgagtcg ctcgccggca tcatgggcgg cgacgccacg
      121 gccgtgtccg acgacacgcg ccacatttac gtcgaggccg ccttctggtg gcccgaggct
      181 gtggccgggc gctcgcgccg ctacaacttc tcgaccgacg ccggccaccg cttcgagcgc
      241 ggcgtcgatc cgcagctcac cgtggaacat atcgagcgca tcacgcaact catcgtcgag
      301 atctgcggca cgcccgagac ggcttgtgcc cccattgatg accagcaggt gaatctgccc
      361 gcgccgaacc cggtgcgcat gcgcgtggcg cgtgccgcca aggtcattgg catgccgctg
      421 acgctggcgc aatgcgtgga tgcgctcaag cgcctgggcc tgccggtgca gcagggcgag
      481 ggcgtgctca ccgtgcagcc accgtccttt cgcttcgacc tgcggctgga ggaggatctg
      541 atcgaggaag tcgcccgcgt ggtcggctac aacaacctgc ccacgacgcc gccgctggcg
      601 cccatcacgc ccaagctacg cctggagtcg caccgcagca gctttgccgt gcgccgcgaa
      661 ctggccgccc tgggctacca ggaaaccatc aacttcagct ttgtcgaaga gcactgggag
      721 aaggagctgg ccggcaatga cacccccatt aagctgctca accccatcgc cagtcagctg
      781 agcgtgatgc gctcgtcgtt gctgggttcg ctcttgcagg tcttgaagtt caacctggat
      841 cgcaaggctg cccgcgtgcg cgtgtttgaa ctgggccgtg tattcctgcg tgatgccagc
      901 gtgaagaaca ccgacaccac ggtggagggc tttcatcagc ccatgcgcgt ggccggtctg
      961 gcctatggcg cgcccgatgc gctgcaatgg ggccgcaagg acaaggccgt ggacttctac
     1021 gacgccaagg gcgacgtgga ggcgctgctg gcgccgctgc gcccaacgtt cgagtcggcc
     1081 acgcaccccg cgctgcaccc gggccgctgc gcccgtgtgc tgctggccgg ccaggccatt
     1141 ggctatgtcg gcgagctgca tccgcagtgg cgccagtcct gggatctgcc gacggcacca
     1201 atgctgttcg agctggagct cgacgccgtg ctgcagcgcc aggtgccggc cttccgcgcc
     1261 gtcgccaagc gtcagctggt ggagcgtgac attgccgtgg tggtggccga gcgcgttacg
     1321 catgacgagg tgatgcgtgc catccgaggc tcgttgccgg gtgaactgtt gcgctcggct
     1381 gtgctgttcg acgtcttccg ccctcagccg caacgcgcag gtgaggctat ggctgctggt
     1441 gccatcgccc agggcgagaa gagcctggcc attcgcctga ccctgggcag cgacgccgag
     1501 gccttgaccg atgcgcaaat tgatgccgcc gtgcagtccg tgctgcaggc gttgacccag
     1561 caaaccgcag cgaggttgcg atgacgatgg aatccgagcc gatggcaatc gagtttgccg
     1621 tggacagtct ggagtcgccg gcgctcacca aggcgcagct ggccgatttg ctgttcgagc
     1681 atatcggcct gaacaagcgc gagtccaagg acatggtgga cgcgtttttc gacctgatcg
     1741 cgcaaagcct gatcgctggc gaagatgtca agctctcggg ttttggcaac ttccagatcc
     1801 gcaccaaggc cgcgcggccc gggcgcaatc cgcgcactgg cgaggccatt cccatcgagg
     1861 cacgccgcgt ggtcaccttc catgccagca gcaagctcaa ggagcagatc caggggtgag
     1921 cggcgccaat gccaggttga cactgctttg cttttgcaac cggtggcaag cctggtggcc
     1981 gcaatagacc tgcagtactc tcaacgcttt cgccgcgtca acgcatcacc atgagccagt
     2041 cgctgcccgc cataccagtc aagcgctact tcacgattgg tgaggtggcg cagctgtgcg
     2101 gcgtcaagcc ccatgtgctg cgctattggg aacaggagtt cacccagctg cgccccatga
     2161 agcgccaggg taaccggcgc tactaccagc accacgaggt gctgatgatc cgccgtatcc
     2221 gcgacctgct gtacgaccag ggtttcacca tcgtcggcgc acgcaaccga ctgcatgagc
     2281 tggcgcaggc agacaaggga gcgcgtgacg ccgcgttgcc gccggatgca ttggacgatc
     2341 agccgattga tcgtgccccc gccttggccc tgccgctgga ctccaatgcg ctcagaacgg
     2401 aattggaaga aattcgcgca cttctttttt agtgctgatt tttcggtata gaatccaagt
     2461 cttgtcggcg tgtagcgcag cctggtagcg cacttgcatg gggtgcaagg g
//
LOCUS       DAOMTQ010000270         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_12404_length_2504_cov_29.997958, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000270 DAOMTQ010000000
VERSION     DAOMTQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12404_length_2504_cov_29.997958"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="PK518_15670"
     CDS             complement(<1..1781)
                     /locus_tag="PK518_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRM50396.1"
                     /translation="MKTTFPHLLLQHAAQRPEAPALREKEYGIWQTWSWQAAATEMRH
                     MACGLLALGFARGQNLALVSDNRPHVYMGFVAVQSIGGVPIPLYQDAVAGEMVFVIED
                     ADIHFAFVENQEQVDKMLEVRESLPGIQYIIYDDPRGLRNYDQPGLISTAELVAKGAE
                     WDRDHPGVWESMVGQVGPQDVSVILYTSGTTGKPKGVCQTHASFIGSASGAAEVDKLG
                     PSDSIISYLPPAWVGDHLFSLAQWLVAGYTINCPESASTISIDMREIGPTYYFAPPRV
                     FEGMLTTISIRMEDAAPFKRRMYEKAMELARRVGSDILDGKPVSSLDRLKYKLADLTI
                     YGPLRNAMGLSRIRVAYTAGAAIGPELFRFFRSIGINLKQFYGQTETCAYVCLQRDGQ
                     VDLNTVGQAAPGIELKLADNSEVLVKGVSVLKEYYKRPDATAEVIDADGWFHTGDAGV
                     IDTHGQLRIIDRAKDVGKLKGGAMFAPNYIENKLKFFSHVKEAVCFGHDKDKVCAFIN
                     IDFDAVGNWAERQNLPYAGYVDLASKAKVLELIADCVAQVNADLAGETGMAETQVARF
                     LVLHKELDPDDDELTRTRKVRRNFIAD"
     gene            2000..>2504
                     /locus_tag="PK518_15675"
     CDS             2000..>2504
                     /locus_tag="PK518_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM50397.1"
                     /translation="MYAPFFGLQHPPFSIAPDPRYLFMSERHREALAHLLYGLDAGGG
                     FVLLTGEIGTGKTTVCRCFLEQIPAHCNVAYIFNPKLTVPELLRSICDEFGVAHQAAA
                     PGVETVKDCLDPLNAFLLQQHAAGRNNVLIIDEAQNLAPDVLEQLRLLTNLETSERKL
                     LQIILIGQ"
BASE COUNT          394 a          831 c          819 g          460 t
ORIGIN      
        1 ttgtcggcga tgaagttgcg ccgcaccttg cgcgtgcgcg tgagctcgtc gtcgtccgga
       61 tccagctcct tgtgcagcac cagaaagcgc gccacctggg tttcggccat gccggtctcg
      121 cccgccaggt cggcgttcac ctgggccacg cagtcggcga tcagctccag caccttggcc
      181 ttggaggcca ggtccacata gccggcgtag ggcaggttct gccgctcggc ccagttgccc
      241 acggcgtcga agtcgatgtt gatgaaggcg cacaccttgt ccttgtcgtg gccgaagcac
      301 acggcctctt tgacgtgcga gaagaacttg agcttgttct cgatgtagtt gggcgcgaac
      361 atggcgccgc ccttgagctt gcccacgtcc ttggcacggt cgatgatgcg cagctggcca
      421 tgggtgtcga tcacgccggc gtcgccggtg tggaaccagc catcggcgtc gatgacctcc
      481 gccgtggcgt cggggcgctt gtagtactcc ttgagcaccg acacgccctt gaccagcacc
      541 tcgctgttgt cggccagctt gagttcgatg ccgggcgcgg cctggcccac ggtgttcaag
      601 tccacctggc cgtcgcgctg caggcagaca taggcgcagg tctcggtctg gccgtagaac
      661 tgcttgaggt tgatgccgat ggagcggaag aagcgaaaca gctccggccc aatggccgcg
      721 cccgcggtgt aggccacgcg gatgcggctc agtcccatgg cgttgcgcag cgggccgtag
      781 atcgtcaggt cggccagctt gtacttcagg cgatccaggc tgctcacggg cttgccgtcc
      841 aggatgtccg agcccacgcg gcgcgccagc tccatggcct tttcgtacat gcggcgcttg
      901 aacggggcgg cgtcctccat gcggatggag atggtggtca gcatgccctc gaacacgcgc
      961 ggcggcgcga agtagtaggt cgggccgatc tcgcgcatgt cgatgctgat ggtgctggcc
     1021 gactccgggc agttgatggt gtagcccgcc accagccact gcgccagcga gaacaggtgg
     1081 tcgcccaccc aggccggcgg caggtaggag atgatgctgt cgctcgggcc gagcttgtcc
     1141 acctcggccg cgccgctggc cgagccgatg aagctggcgt gcgtctggca cacgcccttg
     1201 ggcttgcccg tggtgcccga cgtatagagg atgaccgaca catcctgcgg gccgacctgt
     1261 ccgaccatgg actcccacac gccggggtgg tcacgatccc actcggcgcc cttggccacc
     1321 agctcggcgg tgctgatcag gccgggctgg tcgtagttgc gcaggccgcg cgggtcgtca
     1381 tagatgatgt actggatgcc gggcagggac tcgcgcacct ccagcatctt gtccacctgc
     1441 tcctggttct cgacgaaggc gaaatggatg tccgcgtcct cgatgacgaa gaccatctcg
     1501 ccggccaccg cgtcctggta cagcggaatc ggcaccccgc caatgctttg cacggcgaca
     1561 aaacccatgt acacatgcgg ccggttgtcg ctgaccagtg ccaagttctg cccccgggca
     1621 aagcccagcg ccagcaggcc gcaggccatg tggcgcatct cggtggcggc ggcctgccag
     1681 ctccaggtct gccagatgcc atattctttt tcacgcaggg ccggcgcctc ggggcgctgg
     1741 gcggcgtgct gcagcagtag atgggggaat gtggtcttca tcccggttcc tgttggaatc
     1801 tgtggaaggg gctggcaggc cagggcatgc catcaattgc gagcgatggt aggtggcact
     1861 ttgacatcat tgtgtctgcg cggcgacaat ttgcgggtta atccctaaag gtcaaaccct
     1921 gagcccatcc aggccggcat ggaccagccc gcgtggcgcc agccctggcg catccggtgg
     1981 gggttgtatc ctcgccccca tgtacgcccc gttctttggc ctgcagcacc cgccgttctc
     2041 catcgccccc gatccgcgct acctgttcat gagcgagcgc caccgcgagg cgctggcgca
     2101 tctgctctac ggcctggacg cgggcggcgg cttcgtgctg ctcaccggcg agatcggcac
     2161 cggcaagacc accgtctgcc gctgctttct ggagcagata ccggcgcact gcaatgtggc
     2221 ctacatcttc aaccccaagc tcacggtgcc cgaactgctg cgctcgatct gcgacgagtt
     2281 cggtgtggcg caccaggccg ccgcgcccgg cgtcgagacc gtcaaggact gcctggatcc
     2341 gctcaacgcc ttcttgctgc agcagcatgc cgccgggcgc aacaacgtgc tcatcatcga
     2401 cgaggcgcag aacctggcgc ccgacgtgct ggagcagctg cgcctgctga ccaacctgga
     2461 gaccagcgag cgcaagctgc tgcagatcat cctcatcggc cagc
//
LOCUS       DAOMTQ010000271         2501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_12429_length_2501_cov_16.324203, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000271 DAOMTQ010000000
VERSION     DAOMTQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12429_length_2501_cov_16.324203"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PK518_15680"
     CDS             <1..1016
                     /locus_tag="PK518_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM50398.1"
                     /translation="MMMKKNEKLELRVRLERADVVRNQWDARVQALRTSSLQLQPDRP
                     AVALLAWPLGGDPAQALPLVGAALPVPKGRVGVYVSEAVRDLYGVRPGADWPLLSEVF
                     RPLAQSGQAPTAIFYVAGVWRDYVRQHGAIALDSQDFMRLTGDARASDLALWPAPGAS
                     ADALQAQVLQQARQSLGPAAAGALAFTTAEAIRARSMAMFDRSFAITYWLQAVAIAIG
                     LFGVAASFSAQVLARRKEFGLLAHLGLTRRQILAVVAAEGAAWTAVGAVAGLLLGLAV
                     AAVLVHVVNPQSFHWSMDLALPWARLGALAAAVVAVGTLTAWLAGRQAAGQDAVMAVK
                     EDW"
     gene            1034..2113
                     /locus_tag="PK518_15685"
     CDS             1034..2113
                     /locus_tag="PK518_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HRM50399.1"
                     /translation="MPTITAPPSPSASAWLHHAIARIEADYQRSADTHLIPLPLPAYS
                     AAGIDLYLKDESTHPTGSLKHRLARSLFLYALCNGWVREGRAIVEASSGSTAVSEAYF
                     ARLLGLPFVAVMPRSTSAEKIALIEFYGGRCHFVDGAGEVYDAARAVAAETGGHYMDQ
                     FTYAERATDWRGNNNIAESMFSQMRRERHPVPRWIVVGAGTGGTSATIGRYVRYQRHA
                     TQVCVADPAGSVFAAYHRTGDATLTAPGSRIEGIGRPRVEASFIRTLVDRMDEVQDVD
                     SIVAMRALSALLGRRVGPSTGTNFVAMLRLAQEMRARGEQGSILSLLCDAGERYLPTY
                     HDADWVRRCMGDCAAAEQRLREQLA"
     gene            2110..>2501
                     /locus_tag="PK518_15690"
     CDS             2110..>2501
                     /locus_tag="PK518_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HRM50400.1"
                     /translation="MINRRALLALGVLAWAGPAAALPARTLVFPRDHGSHPDLRTEWW
                     YLTGQARTGPTPGRLWGFQITFFRSRVDATQGLQSAFAARQLLFAHAALTDLQGGRQL
                     HDQRIARAGFGIAQAAEDDTNIHLRDWSL"
BASE COUNT          340 a          903 c          886 g          372 t
ORIGIN      
        1 ccatgatgat gaagaagaac gagaagctcg agctgcgcgt gcgcctcgag cgcgccgacg
       61 tggtgcgcaa ccagtgggac gcgcgcgtgc aggcgctgcg caccagcagc ctgcagctgc
      121 agcccgatcg cccggccgtg gcgctgctgg cgtggccgct gggtggcgac ccggcccagg
      181 ccctgccgct ggtgggcgcg gccctgccgg taccaaaggg gcgcgtgggc gtgtatgtga
      241 gcgaggcggt gcgcgacctg tacggcgtca ggccgggcgc ggactggccc ctgctttcag
      301 aggttttcag gcctctagcg caatcaggac aagcgccaac agctatattt tatgtagctg
      361 gcgtatggcg cgactatgtg cgccagcatg gcgccatcgc cctggacagc caggacttca
      421 tgcgcctgac gggcgacgcg cgcgccagcg acctggcgct gtggcccgcg cccggcgcca
      481 gcgccgatgc cctgcaggcc caggtgctgc aacaggcgcg ccagagcctg gggccggcgg
      541 cggccggcgc gctggccttc accacggccg aggcgatacg cgcgcgctcc atggcaatgt
      601 tcgaccgcag tttcgccatc acctattggc tgcaggcggt ggcgatcgcc atcgggctgt
      661 tcggcgtggc cgccagcttc agcgcccagg tgctggcgcg gcgcaaggag tttggcctgc
      721 tggcccacct gggcctgacg cgccggcaaa tcctggccgt ggtcgccgcc gagggcgcgg
      781 cctggacagc cgtgggcgcc gtcgccggcc tgctgctggg cctggccgtg gcggcggtgc
      841 tggtgcatgt ggtgaacccg cagagctttc actggagcat ggatctggcc ctgccctggg
      901 cgcgcttggg cgcgctggct gccgccgtcg tggccgtggg cacgctgacg gcctggctgg
      961 cgggccgcca ggccgccggg caggacgcgg tcatggccgt caaggaggac tggtagaccg
     1021 cacaatgcgc ctcatgccca ccatcaccgc ccccccatcc cccagcgcca gcgcctggct
     1081 gcaccacgcc atcgcccgca tcgaggccga ctaccagcgc agcgccgaca cacacctgat
     1141 accgctgcca ctgccggcct actccgccgc cggcatcgac ctgtacctga aggacgagtc
     1201 cacccacccc acgggcagcc tcaagcaccg gttggcgcgc tcgctgtttt tgtacgcgct
     1261 gtgcaacggc tgggtgcgcg agggccgggc catcgtcgag gcatccagcg gctccaccgc
     1321 cgtgagcgag gcctacttcg cgcgcctgtt gggcctgccc tttgtggcgg tgatgccgcg
     1381 cagcacctcg gccgaaaaga tcgcgctgat cgagttctac ggcgggcgct gccatttcgt
     1441 ggacggcgca ggcgaggtct acgacgccgc gcgcgccgtg gcggccgaga ccggtggcca
     1501 ttacatggac cagttcacct atgccgagcg cgccaccgac tggcgcggca acaacaacat
     1561 cgccgagagc atgttcagcc agatgcggcg cgagcgccac ccggtgccgc gctggatcgt
     1621 ggtcggcgcc ggcacgggcg gcaccagcgc caccattggt cgctatgtgc gctatcaacg
     1681 gcacgccacc caggtctgcg tggccgaccc ggccggctcg gtgtttgccg cctaccaccg
     1741 cacgggcgac gccacgctca cagcaccggg ctcgcgcatc gagggcatag gccgcccgcg
     1801 cgtggaggcc agcttcatcc gcaccctggt cgatcgcatg gacgaggtgc aggacgtgga
     1861 ttccatcgtc gccatgcgcg ccctgtccgc gctgctgggg cggcgcgtgg ggccgtccac
     1921 cggcaccaac ttcgtcgcca tgctgcgcct ggcgcaggag atgcgtgcgc gcggcgagca
     1981 gggctccatc ctgtcgctgc tgtgcgacgc gggcgagcgc tacctgccca cctaccacga
     2041 cgcggactgg gtgcggcgct gcatgggcga ctgcgcggcg gccgagcagc ggctgcggga
     2101 acagctggct tgatcaaccg ccgcgcgctg ctggccctgg gcgtcctggc ctgggccggg
     2161 ccggcggcgg cgctgccggc gcggacgctg gtctttccgc gcgaccatgg cagccacccg
     2221 gatctgcgca ccgagtggtg gtacctcacc ggccaggcgc gcaccggccc cacgcccggg
     2281 cggctgtggg gctttcagat cacttttttt cgctcgcgcg tcgatgccac gcagggcctg
     2341 caatcggcct ttgccgcgcg ccagctgctc tttgcccatg ccgcgctgac cgacctgcaa
     2401 ggtgggcgcc agctgcacga ccagcgcatc gcgcgcgccg gctttggcat agcgcaggct
     2461 gccgaggacg acaccaacat ccacctgcgc gactggtcgc t
//
LOCUS       DAOMTQ010000272         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_12695_length_2466_cov_25.869349, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000272 DAOMTQ010000000
VERSION     DAOMTQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12695_length_2466_cov_25.869349"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..756)
                     /locus_tag="PK518_15695"
     CDS             complement(76..756)
                     /locus_tag="PK518_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRM50401.1"
                     /translation="MTAAARLAATAALALLTGCATLNPPPPVDLLPTTPPPVAATPRP
                     QVATTGSLFHAASYRPAFEDRRARLVGDIVTIQIVEKVTAKQNSSSNVDRSSETSAGV
                     SAFPFLKANVLGKLGLGAKSENAFSGKGGTENSNTFTGSITATVVEVLPNGHLVVAGE
                     KQIGVNENVDVLRFSGTVDPRSLQPGSVVASTQVANARVQSRGRGAQSEAQAMGWLSR
                     AFNSVAPF"
     gene            complement(767..1549)
                     /gene="flgG"
                     /locus_tag="PK518_15700"
     CDS             complement(767..1549)
                     /gene="flgG"
                     /locus_tag="PK518_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRM50402.1"
                     /translation="MINSLWIAKTGMTAQQTQLDVISHNLSNVSTTGFKRNNAVFEDL
                     IYQNLRQVGAQTSEENQLPTGLHLGLGVRTVATSRNFTQGSLQQSSNSLDVAINGNGF
                     FEVQLPDGTIGYTRDGSFQLDSQGRLVTSGGLPVANGITVPQGATSISISESGVVSAT
                     LAGNPQPQQLGQLAMSSFINAAGLQPVGQNMYKESAASGAPQQGQPGTNGLGVMRQGF
                     LETSNVNVVEELVTMIQTQRAYEMNSKAIQTSDQMLAKLSQL"
     gene            complement(1567..2307)
                     /gene="flgF"
                     /locus_tag="PK518_15705"
     CDS             complement(1567..2307)
                     /gene="flgF"
                     /locus_tag="PK518_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRM50403.1"
                     /translation="MDRIIYTSMTGASAAAHRQAVLSNNLANVSTQGFRAEMANFRSV
                     PLQGSGSTTRVFALEATSGHVDTPGPAQTTGRPLDAMAVGNAWFAVQGLDGTEAYTRA
                     GHFELNAEGQLTTPGGLAVLSDGGAPITAPQGAELSLGADGTISAQIPGQQPQNLGRI
                     KLATPDAQDPLKRGDDGLFRTTSGEPMGNDNQARMVSGALEGSNVNPVEAMVGMIAAA
                     RQFEQQMRLMQTAENDDKSAAQLLSLNG"
     gene            complement(2333..>2466)
                     /locus_tag="PK518_15710"
     CDS             complement(2333..>2466)
                     /locus_tag="PK518_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HRM50404.1"
                     /translation="LEDSNVDLTAELVNMMTAQRAYQANAQTIKTQDQVLSTLVNLR"
BASE COUNT          339 a          796 c          881 g          450 t
ORIGIN      
        1 tacccacgca ataccaccaa gcgctagagg ccaaaattaa ttggaatctg accccaatta
       61 aaccccaatt aacccctaaa acggcgccac cgagttgaac gcccgcgaca gccagcccat
      121 ggcctgggcc tcgctttgcg cgccgcgccc gcgcgactgc acgcgggcgt tggccacctg
      181 ggtggaggcc accacgctgc ccggctgcag cgagcgcgga tccaccgtgc ccgagaagcg
      241 cagcacgtcc acgttctcgt tcacgccgat ctgcttctcg ccggcgacta ccagatggcc
      301 gttgggcagc acctcgacca ccgtggcggt gatggaaccg gtgaaggtgt tgctgttctc
      361 cgtgccgccc ttgcccgaga acgcgttctc gctcttggcc cccaggccca gcttgcccag
      421 cacattcgcc ttcaggaagg ggaaggcaga gacgccggcc gaggtctcgg agctgcgatc
      481 gacgttggag ctggagttct gcttggccgt caccttctcc acgatctgga tggtgacgat
      541 gtcgcccacc agccgcgcgc gccggtcttc aaacgccggg cggtagctgg cggcgtggaa
      601 caggctgccg gtggtcgcca cctggggcct cggggtggcg gccacgggcg gcggcgtggt
      661 gggcagcaga tccaccggcg gcggcgggtt caacgtggcg cagcccgtca gcagggccag
      721 ggcggcggtg gcggcaaggc gcgcggcggc ggtcatggcg gctcccttac agctgcgaca
      781 gcttggccag catctggtcg ctggtctgga tggccttgga gttcatctcg taggcgcgct
      841 gggtctggat catggtcacc agctcctcca ccacgttgac gttggaggtc tccagaaagc
      901 cctggcgcat cacgcccagg ccgttggtgc ccggctggcc ctgctgcggc gcgccggagg
      961 cggcggactc cttgtacatg ttctgcccca cgggctgcag gccggcggcg ttgatgaagc
     1021 tggacatggc cagctgcccc agctgctgcg gctgcgggtt gccggccagc gtggcgctga
     1081 ccacgccgct ctcgctgatg ctgatgctgg tcgcgccctg cggcacggtg atgccgttgg
     1141 ccaccggcag gccgccggag gtgaccaggc gcccctgcga gtcgagctgg aacgaaccgt
     1201 cgcgcgtgta gccaatggtg ccgtcgggca gttgcacttc aaagaaaccg ttgccgttga
     1261 tcgccacgtc caggctgttg ctcgactgct gcaggctgcc ctgggtgaag ttgcggctgg
     1321 tggccacggt gcgcacgccc agccctaagt gcaggccggt ggggagctgg ttttcctcgc
     1381 tggtctgggc gcccacctgg cgcaggttct ggtagatcag gtcttcgaac accgcgttgt
     1441 tgcgcttgaa gccggtggtg gagacgttgg agaggttgtg cgagatcacg tccagctgcg
     1501 tctgctgggc ggtcatgccg gtcttggcga tccacaggga attgatcatg gtgttctcct
     1561 cggtgtttag ccgttcaggc tgagcagctg ggcggcgctc ttgtcgtcgt tctcggcggt
     1621 ctgcatcagg cgcatctgct gctcgaactg gcgcgccgcg gcgatcatgc cgaccatggc
     1681 ctcgaccggg ttcacgttcg agccctccag cgcgccggac accatgcgcg cctggttgtc
     1741 attgcccatg ggctcgcccg aggtggtgcg aaacaggccg tcgtcgccgc gtttgagcgg
     1801 gtcttgtgca tcgggcgtgg ccagtttgat gcgccccagg ttctgcggct gctggccagg
     1861 tatctgggcg ctgatggtgc cgtcggcgcc caggctcagc tcggcgccct gcggcgcggt
     1921 gatgggcgcg ccgccgtcgg acagcacggc caggccgccg ggggtggtga gctggccctc
     1981 ggcgttgagc tcgaaatggc cggcgcgggt ataggcctcg gtgccgtcca gcccctgcac
     2041 ggcaaaccag gcattgccca cggccatggc gtccagcggg cggccggtgg tctgcgccgg
     2101 gccgggggtg tccacatggc cggaggtcgc ctccagcgcg aacacgcgtg tggtcgagcc
     2161 gctgccctgc agcggcaccg agcggaagtt ggccatctcg gcgcgaaagc cctgggtgga
     2221 gacattcgcc aggttgttgg acagcaccgc ctgccggtgc gcggcggcac tggcgccggt
     2281 catgctggtg tagatgatgc gatccatggc ggggtctccc ggtcaggcgt cttcagcgca
     2341 ggttgacgag cgtggacagc acctggtcct gcgtcttgat ggtctgcgcg ttggcctggt
     2401 aggcgcgctg ggcggtcatc atgttcacca gctcggcggt cagatcgacg ttggagtcct
     2461 ccagcg
//
LOCUS       DAOMTQ010000273         2319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_13933_length_2319_cov_22.825530, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000273 DAOMTQ010000000
VERSION     DAOMTQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13933_length_2319_cov_22.825530"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK518_15715"
     CDS             <1..182
                     /locus_tag="PK518_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRM50405.1"
                     /translation="AQSLALLPLRRGAIDSADEPAFGLLVLGSPDPHRFEATMGTDYL
                     ARIAELASAGLTRLL"
     gene            266..1249
                     /locus_tag="PK518_15720"
     CDS             266..1249
                     /locus_tag="PK518_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRM50406.1"
                     /translation="MKPIVVEPGPQLPPEALRYLEHARVEKRLAARTLTLYTLDLQRL
                     VAFAAGVDLPLLRLQSAHIRRFVAQMHAGGRSGRGIALILSGWRGFFTWAARQGLVAH
                     NPVQDVRAPKAPKPLPKALGVDDAVRLAEHAATRADPWLEARDAAMVELLYGCGLRVG
                     ELVGLDLVPSPAAHSQGRGWIDLQAREAHVFGKGSKRRSLPVGQAAARALAHWLALRE
                     QPFGPAGAARLDGALFLGRRGARITAQTVWLRLRQRSQGAGLTTPVHPHMLRHSFASH
                     LLQSSGDLRAVQELLGHASITTTQVYTRLDFQHLAKVYDQAHPRARKKPES"
     gene            1318..1548
                     /locus_tag="PK518_15725"
     CDS             1318..1548
                     /locus_tag="PK518_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HRM50407.1"
                     /translation="MADAWLTTLITDTPQQGFELAITLSRRGVKYTQPDSEVLHKLRP
                     EYANDHAALTAASQVIAMNFQTVAAANNYWRK"
     gene            1638..>2319
                     /locus_tag="PK518_15730"
     CDS             1638..>2319
                     /locus_tag="PK518_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM50408.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRLLTEAHGQKIAVIENEFGEEN
                     IDNDILVTESQEQIVQMSNGCICCTIREDLRETLQLLAAKRRKGLLQFDRVVIETTGL
                     ADPGPVAQTFFMDDEIAETYLIDSIITLVDAKHAQQQLDERQEARRQVGFADQIFLSK
                     TDLVSADETHALIHRIKHMNPRAPIKAVHFGEVPLSEVLDLRGFNLNAKLDIDPDFLK
                     EDDDHDHDH"
BASE COUNT          396 a          800 c          752 g          371 t
ORIGIN      
        1 aggcgcagtc gctggcgctg ctgccgctgc gccgcggcgc catcgacagc gccgacgagc
       61 cggccttcgg cctgctggtg ctgggttcgc ccgacccgca ccgcttcgag gccaccatgg
      121 gcacggacta cctggcgcgc attgccgagc tggcgagtgc cgggttgaca cgcctgctct
      181 gaaaaagaga gttgtcagcg ctgatgtaga gcgggttatc aggggttttg tatctgaaat
      241 catggcatga aaagcgcaac aagctatgaa acccatagtg gttgaacccg gcccgcagct
      301 gccgcccgag gcgttgcgct acctggagca tgcgcgcgtc gaaaagcgcc tggccgcgcg
      361 caccctcacg ctctacaccc tggacctgca gcgcctggtg gcctttgccg cgggtgtgga
      421 tctgccgctg ttgcgcctgc aaagcgcgca tatccgccgc tttgtcgcgc aaatgcatgc
      481 tggcgggcgc agcgggcgcg gcatcgcgct catcctctcg ggctggcgtg gcttcttcac
      541 ctgggccgcg cgccagggcc tggtggcgca caacccggtg caggacgtgc gcgcgcccaa
      601 ggcgcccaag ccgttgccca aggccctggg cgtggacgac gccgtgcgcc tggccgagca
      661 tgcggcgacc cgcgccgacc cctggctgga ggcgcgtgac gcggccatgg tggagctgct
      721 ctacggctgc ggcctgcgcg tgggcgagct ggtcggcctg gacctcgtgc ccagcccggc
      781 ggcgcacagc caggggcgcg gctggatcga tctgcaggcg cgtgaggccc atgtcttcgg
      841 caagggttcc aagcgccgca gcctgcccgt gggccaggcg gcagcccggg cgctggcgca
      901 ctggctggca ctgcgcgagc agccctttgg cccagccggc gcggcgcggc tggatggcgc
      961 gctgttcctg ggccggcgcg gcgcacgcat cacggcgcag accgtctggc tgcggctgcg
     1021 ccagcgcagc cagggcgcgg ggctgaccac gcccgtgcac ccgcacatgc tgcgccactc
     1081 gttcgccagc catctgctgc agtccagcgg cgacctgcgc gccgtgcagg agctgctggg
     1141 ccacgccagc atcaccacca cccaggtcta tacgcggctg gactttcagc atctggccaa
     1201 ggtgtacgac caggcccatc cgcgcgcgcg caaaaagccc gagtcctgac catggtttgt
     1261 gcccgcctgg cgcgggcgtg ggatagtggc cgcttttcag caaaggagaa ccccaccatg
     1321 gcagacgctt ggcttaccac cttgatcacc gacacgcccc agcagggttt cgagctggcc
     1381 atcaccctga gccggcgcgg cgtgaaatac acccagcccg actcggaggt gctgcacaag
     1441 ctgcgccccg agtacgccaa cgaccatgcg gcgctcacgg ccgcgtcgca ggtgatcgcc
     1501 atgaacttcc agaccgtggc cgcggccaac aattactggc gcaagtaatg gcttgacagg
     1561 ctggctacac tgagtctctg gtttgtttcg cggcggccat ccggtcgccg tttgcatttg
     1621 ttgacggaaa tacctgtatg tccctgatcc ccgccaccat cctcaccggc ttcctcggct
     1681 cgggcaagac cacgctgctc aagcgcctgc tcaccgaggc ccatggccag aagattgccg
     1741 tgatcgaaaa cgagtttggc gaggagaaca tcgacaacga catcctggtc accgaaagcc
     1801 aggagcagat cgtgcagatg agcaacggct gcatctgctg caccatccgc gaggatctgc
     1861 gcgagacgct gcagctgctg gccgccaagc gccgcaaggg cctgctgcag ttcgaccgcg
     1921 tggtcataga gaccaccggc ctggccgacc ccggccccgt ggcacagacc ttcttcatgg
     1981 acgacgagat cgccgagacc tacctcatcg actccatcat caccctggtg gacgccaagc
     2041 atgcccagca gcagctcgac gagcgccagg aggcgcggcg ccaggtgggc tttgccgacc
     2101 agatcttcct gtccaagacc gacctggtga gcgccgacga gacccatgcg ttgatccacc
     2161 gcatcaagca catgaacccg cgcgcgccga tcaaggccgt gcattttggc gaggtgcctt
     2221 tgagtgaggt gctggatctg cgcggcttca acctgaatgc caagctggac atagaccccg
     2281 acttcctcaa ggaagacgac gaccatgacc atgaccatg
//
LOCUS       DAOMTQ010000274         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_14048_length_2305_cov_17.335111, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000274 DAOMTQ010000000
VERSION     DAOMTQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14048_length_2305_cov_17.335111"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2305)
                     /locus_tag="PK518_15735"
     CDS             complement(<1..>2305)
                     /locus_tag="PK518_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50409.1"
                     /translation="RLALAREPRLLAVARLRNQLTRWLRGALLACLLLAGAGALDWAS
                     WWLLLMLSSSQPSGWVWGGVGAGGALLFVLRGLAPVLQQLAAQAEARHTMALGPLLLN
                     VAGHLGALVLLLAWLLAVQWWVFAEAPLALLRGFPAWMRWAMLALAALGWWLTTARLA
                     QAANASSLSGFYQARLVRAWLAVGNRRRGICTPFTPADARLRSVTEVAAGDDVDLTCH
                     HPEADGGPLHLIGACLNQTRDDASGLFNADRKGRLVIASARALEVGTQEVLRWDDGAA
                     TASAGRAAGTLGGWVAVSGAAAAPGAGAYTSRGWALVMFLLGIRLGHWIESPLPQALT
                     PLKTWLWRRGPKPLMLWSEARARFFGPARPWWYLSDGGHFDNTGVYALIKRECDFIIL
                     SDASADPEYRFADIENLVRKARIDLDAEIEFYSRQEAARLLSLPGDNLTVLSPDELAD
                     NSTRRGVLLARIRYRHSDPARGEREGTLLVVKPNLHVGLDLDLLSYAQRHPSFPHEST
                     GDQFFDEAQWESYQRLGQDLGQAVYDRWLAQLPGWDRVQLHKLALPERLQQPRETQAP
                     PAPDAQARLPRWRRSVRAAAIGTSVGVGVTGTLLLSLWQLQEQLNRSTDARQAEISKL
                     LNDSSRELLEVSGSCRRVGAHTLGHVEQLRALDGSEALSPAEQITVKRLLDRLNEQCT
                     GNAQAIAACAETERQHQKDLCEVAQKKIGSGSALNYWQPLPRPPDAIHNLAQAWALLR
                     GREQSASVAPAPAAPVSTPAPAPTATST"
BASE COUNT          310 a          853 c          847 g          295 t
ORIGIN      
        1 ggtgctggtc gccgtgggcg ccggcgcggg cgtggacacg ggcgcggctg gggccggggc
       61 cacgctggcg gactgctccc ggccgcgcag cagcgcccat gcctgcgcca ggttgtggat
      121 ggcgtcgggg ggccggggca ggggctgcca gtagttcagc gcactgccgc tgccgatttt
      181 cttttgcgcg acctcgcaca agtccttctg gtgctggcgt tcggtctcgg cgcaggcggc
      241 aatggcctgg gcgttgcccg tgcattgttc gttcaggcga tcgagcagcc gcttcacggt
      301 gatctgctcg gccgggctca gggcctcgct gccgtccagg gcgcgcagct gctccacatg
      361 gcccagggtg tgcgcgccca cgcgcctgca gctgccggac acctcgagca gctcgcgcga
      421 ggagtcgttc agcagcttgc tgatctcggc ctggcgcgcg tcggtgctgc ggttgagctg
      481 ctcctgcagc tgccacagcg acagcagcag cgtgccggtc acgcccacgc cgaccgatgt
      541 gcctatggcc gccgcacgca cgctgcggcg ccagcgcggc aggcgcgcct gggcgtccgg
      601 cgccggcggt gcctgggtct cgcgcggctg ctgcaggcgc tcgggcaggg ccagcttgtg
      661 cagctgcacc ctgtcccagc cgggcagctg ggccagccag cggtcgtaca cggcctggcc
      721 caggtcctgg cccaggcgtt ggtagctctc ccactgcgcc tcgtcgaaga actggtcgcc
      781 cgtggactcg tgcggaaagc tggggtggcg ctgggcgtag gacagcaggt ccagatccag
      841 gccgacatgc aggttgggct tgacgaccag cagcgtgccc tcgcgctcgc cgcgcgccgg
      901 gtcgctgtgg cggtagcgta tgcgcgccag cagcacgccg cggcgcgtgc tgttgtcggc
      961 cagctcgtcg ggcgagagca cggtcaggtt gtcaccgggc aggctgagca ggcgcgcggc
     1021 ctcctggcgg ctgtagaact cgatctcggc gtccaggtcg atgcgcgcct tgcgcaccag
     1081 gttctcgatg tcggcaaagc ggtactcggg gtcggcgctg gcgtccgaca ggatgatgaa
     1141 gtcgcactcg cgcttgatca gcgcgtacac gccggtgttg tcgaaatgcc cgccgtccga
     1201 cagataccac cagggccgcg ccggcccgaa gaaccgggcg cgcgcctcgc tccagagcat
     1261 cagcggcttg ggcccgcgcc gccacaacca ggtcttcagc ggtgtcagcg cctggggcag
     1321 gggcgactct atccagtggc ccaggcgtat gccgagcaga aacatcacca gcgcccagcc
     1381 gcgcgaggtg taggcgcccg cgcccggcgc cgccgccgcg cccgagacgg ccacccagcc
     1441 gcccagcgtg ccggcggcgc ggccggcgct ggccgtggcg gcgccgtcgt cccagcgcag
     1501 cacctcttgc gtgccgacct ccagcgcgcg cgccgaggcg atcaccagcc gccccttgcg
     1561 gtcggcgttg aacaggcccg aggcgtcgtc acgcgtctgg ttcaggcagg cgcctatcag
     1621 gtgcagcggg ccgccgtcgg cctcggggtg gtggcaggtc agatccacgt cgtcgcccgc
     1681 ggccacctcg gtcacgctgc gcaggcgggc atcggccggc gtgaagggcg tgcagatgcc
     1741 gcgccgccgg ttgcccaccg ccagccaggc gcgcaccagg cgcgcctggt agaagcccga
     1801 cagcgaggag gcattcgccg cctgcgccag ccgcgccgtg gtcagccacc accccagggc
     1861 cgccagcgcc agcatggccc agcgcatcca ggccggaaag ccgcgcagca gcgccagcgg
     1921 ggcctcggca aacacccacc actgcacggc cagcagccag gccagcagca gcaccagggc
     1981 ccccaggtgg ccggcaacgt tcagcagcag cgggcccagc gccatggtgt ggcgcgcctc
     2041 ggcctgggcc gccagctgtt gcaagaccgg cgccagcccg cgcagcacga acagcagggc
     2101 gccgcccgcg cccacgccgc cccagaccca gcccgacggc tggctgctgg agagcatcag
     2161 cagcagccac cagctggccc agtccagcgc gccggcgccg gccagcagca gacaggccag
     2221 cagggcaccg cgcagccacc gggtcagctg gttgcgcagc cgcgccacgg ccagcaggcg
     2281 cggctcgcgg gccagcgcca ggcgg
//
LOCUS       DAOMTQ010000275         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_14519_length_2259_cov_23.882486, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000275 DAOMTQ010000000
VERSION     DAOMTQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14519_length_2259_cov_23.882486"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..488
                     /locus_tag="PK518_15740"
                     /pseudo
     CDS             33..488
                     /locus_tag="PK518_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
     gene            complement(617..2077)
                     /locus_tag="PK518_15745"
     CDS             complement(617..2077)
                     /locus_tag="PK518_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM50410.1"
                     /translation="MLTRSPDRSLTEQLAERFAERIRTRLLPAGARLPSVRECARQQG
                     VSPHTVVAAYDKLLAQGLVEARRQRGFYVRDIVQKMPLAQDGQAQAAILSEATAASPL
                     GPMGLCLPGSRINATALIRGMFHQVSNKPQPGSGVLPVAWLEEARFLSAAVRKITAGP
                     ALQGGSLSYGEPMGDAGLRQALARRMADIHIPARPGQIITTVGATQALDIVSRTMLKA
                     GDPVMVEEPGWSVEFARLEALGMRVLPVPRGPEGPDLAVMARYCELHAPRLFVSVSVL
                     HNPTGYSLSPAGAHQVLQLAQRHDFHIVEDDTYGHIAPEHATRLSMLDGLRRTIYVSG
                     FAKILAPNWRIGYLAAPPDLVERMLDTKLLSTLTTPAILEKALALCIDQGQLRRHAER
                     MRQRIAQARTRSVQLALAAGCRFAAEPAGMFGWVDTGVDTDMLAQRLLDEGYLIAPGA
                     LFHATRQPCTLMRINFGNTQEPAFWRAWQRVVASVK"
BASE COUNT          321 a          787 c          808 g          343 t
ORIGIN      
        1 ggagggcgta acggaggatg aggccatcaa tgatgcccgt ggccgccgcc ggcggcctgg
       61 ccgatggccg tgacgtggcg cgcgtgctgg cccagggcgc ccaggccgcc gtgctgggca
      121 cggccctgat cgccacgccg gaatcctttg cccacgacta ccacaagcgc cgcctggtcg
      181 ccgcccgcgc cagcgacacc gtgctgaccg aagacttcca catcaactgg ccgccccacg
      241 ccgccgtgcg cgtgctggcg agcagcgtca cccgcggcga gcgcggcgac cccttcagcg
      301 ccgccgcccg caccaccatt ggcgaagaag agggccggcc catctacctg ttcagcaccg
      361 actcgccgct gcgctccatg acgggcgatt tcgaaggcat ggccctgtac gccggccggg
      421 gcgtggatcg catcggcgac gtagagcccg cgggcgagcg cgtgcggcgc attgccgagg
      481 atgcggcgca ggagctgcac ccggcctgct gacccggcaa caaagggttg accgtcatcc
      541 cggcgaaagc cgggatccag gggcaccctt ggccagcgcg gccgttgaat ccctcttcgc
      601 tcgctggcca ggcggcttat ttcaccgacg ccaccacccg ctgccacgcc cgccagaacg
      661 ccggctcctg cgtgttacca aagttgatgc gcatcagcgt gcagggctgg cgcgtggcgt
      721 ggaacagggc gccgggggcg atcaggtagc cctcgtcgag caggcgctgg gccagcatgt
      781 cggtgtccac gccggtgtcc acccagccga acatgccagc gggctcggcg gcgaagcggc
      841 agccggccgc cagggccagc tgcacgctgc gcgtgcgcgc ctgggcgatg cgctggcgca
      901 tgcgctcggc gtggcggcgc agctggccct ggtctatgca cagcgccagc gccttttcca
      961 ggatggcggg ggtggtcagg gtggacagca gtttggtgtc cagcatgcgc tccaccaggt
     1021 cgggcggcgc cgccaggtag cctatgcgcc agttgggcgc cagaatcttg gcgaaaccgc
     1081 tcacatagat ggtgcggcgc aggccgtcga gcatggacag gcgcgtggcg tgctcgggcg
     1141 cgatatggcc gtaggtgtcg tcctcgacga tgtgaaagtc gtgccgctgc gccagctgca
     1201 gcacctggtg cgcgccggcc ggcgacaggc tgtagcccgt ggggttgtgc agcacgctga
     1261 cgctgacgaa cagcctgggc gcgtgcagct cgcagtagcg cgccatgacg gccaggtccg
     1321 gcccctcggg gccgcgcggc acgggcagca cgcgcatgcc cagggcttcg aggcgcgcga
     1381 actccacgct ccagccgggc tcctccacca tcaccgggtc gccggccttg agcatcgtgc
     1441 ggctgacgat gtccagtgcc tgcgtggcgc ccacggtggt gatgatctgc ccgggccggg
     1501 cggggatgtg gatgtcggcc atgcgccgcg ccagcgcctg gcgcaggccg gcgtcgccca
     1561 tgggctcgcc gtagctcagc gagccgccct gcagcgccgg gccggcggtg atcttgcgca
     1621 cggcggcgct caggaagcgc gcctcttcca gccaggccac gggcagcacg cccgagccag
     1681 gctggggctt gttcgagacc tggtggaaca tgccgcggat cagcgccgtg gcgttgatcc
     1741 gcgagcccgg caggcacagg cccatggggc ccagtgggga tgccgctgtt gcttctgaaa
     1801 gaatagctgc ctgcgcttgc ccatcctgcg ctagcggcat tttttgcact atgtcgcgca
     1861 catagaagcc gcgctggcgc cgagcctcca ccagcccctg ggccagcagc ttgtcgtagg
     1921 ccgcgaccac ggtgtggggg ctcacgccct gctggcgcgc gcattcgcgc accgagggca
     1981 ggcgcgcgcc ggcgggcagc aggcgcgtgc gtatgcgctc ggcaaagcgc tcggcgagtt
     2041 gttcggtcag gctgcggtcg gggctgcggg taagcatggc tgtgtcagtg gatgaaccgg
     2101 tacagttgct tcatgtgtgg gtttgactgt accggtactg taatggcgtc tgcccctaca
     2161 gtacagcctt cctgcccctg ccccacaacg ccccatgccc ctggttgaat tcaccgccct
     2221 gctggtgctg gccaccgcga tgagcttcac gcccggccc
//
LOCUS       DAOMTQ010000276         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_14666_length_2246_cov_24.600183, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000276 DAOMTQ010000000
VERSION     DAOMTQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14666_length_2246_cov_24.600183"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..393)
                     /locus_tag="PK518_15750"
     CDS             complement(169..393)
                     /locus_tag="PK518_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HRM50411.1"
                     /translation="MFPEYRDLITKIKGQDGHFTRLFDKHNELDQLIKNMEARIVAAT
                     IHEIEKLKKEKLLLKDQIYAHLRRVDGAQA"
     gene            596..1474
                     /locus_tag="PK518_15755"
     CDS             596..1474
                     /locus_tag="PK518_15755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HRM50412.1"
                     /translation="MQRLYIKRLQLCFLMILGCMAAHAAPPSPTLAQRYHPGIDVTAY
                     RVSEKYDGVRALWTGSQLQSRQGLPIRAPAWFTAGWPAEPLDGELWAGRGRFAAAQAA
                     VAAGTPDDAQWRGLRYMVFDVPSAPGGFDARLPTLQRQVEAIGQPWVQAVPQWRVASH
                     GELMQQLRAHDKAGAEGLMLRRGDAPYRGGRSEDLLKLKSFDDAEGLVVGHVPGRGKY
                     AGQTGALLVQMPDGQRFRLGSGLSDALRRDPPPLGTLVTYRFNGTHAGGLPRFARFWR
                     VRADQPPGEKTSQIGP"
     gene            complement(1669..>2246)
                     /locus_tag="PK518_15760"
     CDS             complement(1669..>2246)
                     /locus_tag="PK518_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HRM50413.1"
                     /translation="QQFKDDYEKNQPMWRILPEFCQQHKRYEKMGLRDLCQHVHELYA
                     KFDIARLTTEMYLSDLTPAMKPSDAYAHIAQRKTERVPVDELEGRITTSLITPYPPGI
                     PLLIPGEVFNRKIVDYLKFAREFAKLCPGFETDIHGLVEIEDESGNVHYYADCVAQPG
                     ATAAPKARSARASAKDKLVQVGSDGPFGRTL"
BASE COUNT          396 a          709 c          715 g          426 t
ORIGIN      
        1 tcattcgggc caatagccga ttaagctcca aacagaatgg aacactacac tagcttcgcg
       61 tagtccgctg ccccccgagg ggggcttttc atcttggggc ggcccggcaa tgaaaaaacc
      121 ctgccagcaa caatggcctg cggggctgga tggttcaggc aggcagcatc aggcttgcgc
      181 cccgtccacg cgtcgcaggt gcgcgtagat ctgatccttg agcagcagct tctccttctt
      241 cagtttctcg atctcatgga tcgtggcggc gacgatgcgc gcctccatgt tcttgatcag
      301 ttggtccagc tcgttgtgct tgtcaaacag gcgcgtgaag tggccgtcct ggcccttgat
      361 cttggtaatc aggtcgcgat actcgggaaa catcggtgct ccttaggggg gtgaatgacg
      421 cctacagttt acgggctcgc ccccgcctcc cgaattcacc accgaggccg ccgcaccacc
      481 gctgcccctg tgcaagcatg ttcatgcggc ctcgacaagc cacccgggag atcccatgcg
      541 ccgcgttgat gccactttgc gcccgaaccg ccatgatact gagcatgttt ttcacatgca
      601 gcgcttatat ataaagcgtt tgcagctatg ttttttgatg attttgggtt gcatggcggc
      661 gcatgccgct ccgccctcgc ccacgttggc acagcgttac caccctggaa tagacgtgac
      721 cgcctaccgg gtcagcgaga aatacgacgg cgtacgcgcg ctgtggacgg gcagccagct
      781 gcaaagccgc cagggcctgc cgattcgcgc acccgcctgg tttaccgccg gctggccggc
      841 agagcccctg gatggcgagc tgtgggccgg acgcggccgg ttcgccgccg cgcaggccgc
      901 agtggcggcc ggcacgcccg acgacgcgca gtggcgcggc ctgcgctaca tggtgttcga
      961 tgtgcccagc gcgccgggtg gtttcgatgc ccggctgccc actctgcaac gccaggtcga
     1021 agccattggc cagccctggg tgcaggcggt gccgcagtgg cgcgtggcca gccatggcga
     1081 gctgatgcag cagctgcgcg cccacgacaa ggccggcgcc gagggcctga tgctgcgccg
     1141 gggcgatgca ccctaccgcg gcgggcgcag cgaggatttg ctcaagctca agtccttcga
     1201 cgatgcggaa ggcctggtgg tgggccatgt accgggacgc ggcaagtacg cgggccagac
     1261 cggggcgctg ctggtgcaaa tgccggacgg ccagcgcttt cgcctgggca gcggcctgag
     1321 cgatgcactg cgccgcgatc cgccaccgct gggcacgctg gtgacctacc gcttcaacgg
     1381 cacgcacgcc ggcggtctgc cacgctttgc gcgcttctgg cgcgtgcggg cggatcagcc
     1441 accgggtgaa aaaactagcc aaatcgggcc ctagcgcccg tccataaagc gctatcagct
     1501 atcaaaacaa tggctcaagt agcgattcct attgacgccg aatgacaagc ctaccgagcg
     1561 tggcacgggc caatcaacag atatcgctac ttgagccaaa acaatagaaa aaaccgaggg
     1621 ccatagctca ccctcggttc tggcctgacc ggtatgccag gtggcgcgtc agagggtgcg
     1681 gccgaagggg ccgtcgctgc ccacttgcac cagcttatcc ttggccgatg cacgcgcgct
     1741 acgcgccttg ggcgcagcgg tggcgcccgg ctgggccacg cagtcagcgt agtagtgcac
     1801 gttaccgctt tcgtcctcga tctccaccag gccgtggatg tcggtttcga aaccgggaca
     1861 cagcttggcg aactcgcgcg cgaacttgag gtagtccacg atcttcctgt tgaagacctc
     1921 acccgggatc aggagcggaa tacccggcgg gtacggcgtg atcagactgg tggtgatgcg
     1981 gccttccagc tcatcgacgg gcacgcgctc ggtcttgcgc tgggcaatgt gggcataggc
     2041 gtcgctgggc ttcatggccg gggtcaggtc tgagaggtac atctcggtcg tcaggcgcgc
     2101 gatgtcgaac tttgcgtata gctcgtgcac atgctggcac aggtcacgca agcccatctt
     2161 ctcgtagcgt ttgtgctgct ggcaaaactc tggaaggatg cgccacatcg gctggttctt
     2221 ctcgtagtcg tccttgaact gctgca
//
LOCUS       DAOMTQ010000277         2211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_15018_length_2211_cov_23.219852, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000277 DAOMTQ010000000
VERSION     DAOMTQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15018_length_2211_cov_23.219852"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PK518_15765"
     CDS             complement(<1..818)
                     /locus_tag="PK518_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRM50414.1"
                     /translation="MSIFTTASLLDSCGCCTGRIWHARRAFLLAAAGAAALPVAAQVD
                     VGKSSAMRQLVPAEQLETSATQQYDQMLAQAKAKRALAPDNHPQLIKLRAIAQRIIPY
                     AQQWNSRAGSWRWEVNLIGSKQINAFCMPGGKIAFYTGILDQLKLTDDEIAMVMGHEM
                     AHALREHARERIAKSQATSIGLSLGAQLLGLGELGNMAANLGTQLLTLKFSRSDESEA
                     DLVGLELAARAGYDPQAAVTLWQKMGQATGNGGIGFLSTHPTGPDRIRELEQNVP"
     gene            complement(829..1710)
                     /locus_tag="PK518_15770"
     CDS             complement(829..1710)
                     /locus_tag="PK518_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM50415.1"
                     /translation="MNDTRHSARGMALVLLAAMLWGTTGTAQSLAPATLSPYWVGALR
                     LVIGSAFFAVLAWRAPARSGPWPWAQMALAGGCIAVYNLSFFAGVKQSGVALGTALAI
                     GSGPIWAGLLQTLLARRLPPALWWLGMLVSVAGGAAMALGQGGELRLNATGVALCLLA
                     GLAYASYTLVNKPLVLRLGPARANLAVFAGAALLSVPVAWVLGGPLQAGAAAWGVVLF
                     LGLVSTGLSYLLFSSGLRHISGTTGVTLALGEPVTAFVLAVWVVGERQQPLAWLGLAG
                     VIAGLLLVVWAELRAAP"
     gene            complement(1841..>2211)
                     /locus_tag="PK518_15775"
     CDS             complement(1841..>2211)
                     /locus_tag="PK518_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM50416.1"
                     /translation="AAHLALLLQAPRVLARRGLDLDLLRAAVEGALHQDWPTARMAAL
                     FLLSPQRFHARLLELTGHTPQQWLRARRLDVAERALARGQSLEATALRTGYASASALA
                     YALRRERGQGARVLRRGDAI"
BASE COUNT          350 a          816 c          759 g          286 t
ORIGIN      
        1 ggcacgttct gctccagctc gcggatgcgg tctggccccg tggggtgggt ggacagaaat
       61 cctatgccgc cgttgcccgt ggcctggccc atcttctgcc acagggtcac ggcggcctgc
      121 gggtcgtagc cggcgcgcgc ggccagctcc aggcccacca ggtcggcctc gctctcgtcg
      181 ctgcggctga atttgagcgt caaaagctgc gtgcccaggt tggccgccat attgcccagc
      241 tcgcccaggc ccagcagctg cgcgcccagc gacaggccga tgctggtggc ctggctcttg
      301 gcgatgcgct cgcgcgcgtg ctcgcgcagg gcatgcgcca tctcgtggcc catgaccatg
      361 gcgatctcgt cgtcggtcag tttcagctga tccaggatgc cggtgtagaa ggcgatcttg
      421 ccgccgggca tgcagaaggc attgatctgc ttgctaccga tcaggttcac ctcccagcgc
      481 cagctgccgg cgcggctgtt ccactgctgg gcgtagggaa tgatgcgctg cgcgatggcg
      541 cgcagcttga tgagctgcgg gtggttgtcc ggcgccagcg cgcgcttggc cttggcctgg
      601 gccagcatct ggtcgtactg ctgggtggcc gaggtctcca gctgctctgc cggcaccagc
      661 tggcgcatgg ccgacgactt gcccacgtcc acctgggccg ctaccggcag ggcggcggcg
      721 ccagcggcgg ccagcaggaa ggcgcggcgc gcgtgccaga tgcggccggt gcagcagccg
      781 cagctgtcca agagtgaagc ggtggtaaag atgctcatgc gcgcaagtct acggcgcggc
      841 gcgcagctcc gcccacacca ccagcaacag cccggcgatc accccggcca gtcccagcca
      901 ggccagtggc tgctggcgct cgcccaccac ccacaccgcc agcacaaagg ccgtcaccgg
      961 ctcgcccagc gccagcgtca cgccggtggt gcccgagata tggcgcagcc cgctggagaa
     1021 caacagatac gacagcccgg tgctgaccag ccccagaaac agcaccacgc cccaggccgc
     1081 agcccccgcc tgcaatggcc cgcccaacac ccaggccacc ggcaccgaca gcagcgccgc
     1141 gccggcaaac accgccagat tggcgcgcgc cgggcccagg cgcagcacca gcggcttgtt
     1201 caccagggtg tagctggcat aggccagtcc ggccagcagg cacagcgcca cgcccgtggc
     1261 gttcagccgc agctccccac cctggcccag cgccatggcg gcgccgccgg ccacgctgac
     1321 cagcatgccc agccaccaca gcgcgggcgg caggcgccgc gccaacagcg tctgcagcag
     1381 cccggcccag atggggccac tgccaatggc aagggccgtg cccagggcca cgccgctttg
     1441 cttgacgccg gcaaaaaagc tcaggttgta gacggcgatg cagccgccgg ccagggccat
     1501 ctgcgcccag ggccaggggc cactgcgcgc cggtgcgcgc caggccagga cagcaaaaaa
     1561 ggcgctgcca atcaccagcc gcagcgcccc cacccagtag ggcgagagcg tggccggcgc
     1621 caggctttgc gccgtgccgg tcgtgcccca gagcatggcg gccagcagca ccagggccat
     1681 gccgcgggcg gaatgtcgtg tgtcgttcat gcctgccatg ctgcgcccgg attggcgggc
     1741 aactatcgag caacgcgcgt agtgcgggcc gcctgggcgg cggaagaccc aagcaaaata
     1801 cggcctttgc gcccgccaat aaagcgctaa cagctattta ttaaatagca tcaccgcgcc
     1861 gcagcacccg cgccccctgg ccgcgctcgc gccgcagcgc gtaggccagg gcgctggcgc
     1921 tggcgtagcc ggtgcgcagg gccgtggctt cgagcgactg gccgcgggcc agggcgcgct
     1981 cggccacatc cagccggcgc gcgcgcagcc attgctgggg cgtgtggccg gtcagctcca
     2041 gcaggcgcgc gtgaaagcgt tgtgggctca gcaggaacag cgccgccatg cgcgccgtcg
     2101 gccagtcctg gtgcagcgcg ccctccacgg cggcgcgcag caggtccaga tccagccccc
     2161 ggcgcgccag cacgcgcggc gcctgcagca gcagggccag atgggccgcc g
//
LOCUS       DAOMTQ010000278         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_15152_length_2199_cov_31.954291, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000278 DAOMTQ010000000
VERSION     DAOMTQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15152_length_2199_cov_31.954291"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1065)
                     /locus_tag="PK518_15780"
     CDS             complement(91..1065)
                     /locus_tag="PK518_15780"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRM50417.1"
                     /translation="MKTRITELFGIEHPIIQGGMHYVGYAELASAVSNAGGLGLITAL
                     TQKTPALLAQEIARCREMTDKPFGVNLTFLPAVNAPDYPGYVKAIIEGGVKIVETAGN
                     NPQKWLPTLKDAGIKVIHKCTSVRHALKAESIGCDAISVDGFECAGHPGEDDIPNFIL
                     LPRAAEELKIPFVASGGMADGRSLVAALALGAEGINMGTRFIATKEAPVHQNVKDTIV
                     AASELDTRLIMRGLRNTERVLISHATERVMAKERELGSAITFADIAPELAGVYPRIMQ
                     QGDMEAGVWSCGMVAGLIRDVPSVQELITRIMAEAESIIASRLAGLLR"
     gene            complement(1073..2068)
                     /locus_tag="PK518_15785"
     CDS             complement(1073..2068)
                     /locus_tag="PK518_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM50418.1"
                     /translation="MKPNAIHRRAALALAAAPLLAGICGGALAQAAWPDKMIKLIVPF
                     PAGGPTDTASRIVGQKLAERLKQPVVVENRPGASGSIAAVQVAKSPADGYTLMMLATP
                     TLLAPHLYKKAGYDTVKDFAPVATVYDLPIVIVVNPRLLPDVTDLKGLIAHAKAQKTP
                     INYTSSGAGSFGHLSMELLKQMAGFDMQHIPYKGGVPAITDTIGGQVPIMYADLVAAL
                     PHIKAGKLRAIAVGSPQRVVMLPEVKTIAEQGVKGYDAVSWGGLLAPQGTPKAVVDRI
                     ATEVQQILADKEVQERLLGAGAIARFEDPAQMGQRIAQDYQRWGQIIRDKGISPE"
BASE COUNT          336 a          710 c          752 g          401 t
ORIGIN      
        1 cgtccgattt cacgcggacc ttcacgttgt agtccaccac gcgtttgacg gggacgagga
       61 ttttcataag ttgctcctaa caaaaaaagg tcagcgcagc aggccggcga ggcggctggc
      121 aatgatggat tcggcctcgg ccatgatgcg ggtgatgagc tcctgcacgc tgggcacgtc
      181 gcggatcagg ccggccacca tgccgcagga ccacacgccc gcctccatgt cgccctgctg
      241 catgatgcgc ggatagacgc cggcgagctc gggggcgatg tcggcaaagg tgatggcgct
      301 gcccagctcg cgctccttgg ccatcacacg ctcggtggca tggctgatca gcacgcgctc
      361 ggtgttgcgc aggccgcgca tgatcaggcg cgtgtccagc tcgctggcgg cgacgatggt
      421 gtccttgacg ttctggtgca ccggcgcctc tttcgtggcg atgaagcgcg tgcccatgtt
      481 gatgccctcg gcccccaggg ccagcgcggc caccaggctg cggccgtcgg ccatgccgcc
      541 ggaggcgacg aagggaatct tcagctcctc ggccgcgcgc ggcagcagga tgaagttggg
      601 gatgtcgtcc tcgcccgggt ggccggcgca ttcaaagccg tccacgctga tggcgtcgca
      661 accaatcgat tcggccttga gcgcatggcg caccgaggtg catttgtgga tcaccttgat
      721 gccggcatcc ttcaatgtcg gcagccactt ttgcgggttg ttgccggccg tctcgacgat
      781 cttcacgccg ccttcgatga tggccttgac atagcccgga tagtccggcg cgttcaccgc
      841 tggcagaaag gtcagattca cgccaaaggg cttgtcggtc atctcgcggc agcgcgcgat
      901 ttcctgcgcc agcagcgcgg gcgtcttttg cgtcagcgcc gtgatcaggc ccaggccgcc
      961 ggcgttggac acggccgatg ccagctcggc gtagcccacg taatgcatgc cgccctggat
     1021 gatggggtgc tctatgccga agagctctgt gatgcgggtc ttcatatgct gtttactccg
     1081 gcgaaatgcc cttgtcgcgg atgatctggc cccagcgctg gtagtcctgc gcgatgcgct
     1141 ggcccatttg cgcgggatct tcgaagcggg cgatggcgcc cgcgcccaac aggcgctcct
     1201 gcacctcctt gtcggccagg atctgctgca cctcggtggc gatgcgatcc accactgcct
     1261 tgggcgtgcc ctgcggcgcc agcaggccgc cccaggaaac ggcgtcgtag cccttcacac
     1321 cctgctcggc gatggtcttc acctcgggca gcatcaccac gcgctggggc gagcccacgg
     1381 cgatggcgcg cagcttgccg gccttgatgt gcggcaaggc cgccaccagg tcggcataca
     1441 tgatgggcac ctggccgcct atggtgtcgg tgatggccgg cacgccgccc ttgtagggga
     1501 tgtgctgcat gtcaaaaccc gccatctgct tgagcagctc catgctcagg tggccgaagc
     1561 tgccggcacc cgagctggtg tagttgatcg gcgtcttttg cgccttggcg tgggctatca
     1621 agcccttgag gtcggtcaca tcgggcagca gcctggggtt caccacgatg acgatgggca
     1681 ggtcgtacac cgtggccacg ggcgcgaagt ccttcaccgt gtcgtagccg gccttcttgt
     1741 acaggtgcgg cgccagcagc gtgggcgtgg ccagcatcat cagcgtgtag ccgtcggccg
     1801 ggctcttggc gacctggacg gcggcgatgg agccggaggc gccggggcga ttttccacca
     1861 ccacgggctg cttcaggcgc tcggcgagct tctggcccac gatgcgcgag gccgtgtcgg
     1921 tggggccgcc ggccgggaag ggcacgatga gcttgatcat cttgtcgggc caggcagcct
     1981 gggccagggc gccgccgcaa atgccggcga gcagcggcgc ggcggccagg gccagggcgg
     2041 cgcggcgatg gatggcgttg ggtttcatat ggatctcctg gagcaaataa gcctgatgag
     2101 cgacatgcct caggccaggc cgcgagccct cacccccgcc ctctcccaaa gggagaggga
     2161 gtaaaaccgg cgcaaccggt cgccctcgcc cctttgggg
//
LOCUS       DAOMTQ010000279         2189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_15257_length_2189_cov_21.225398, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000279 DAOMTQ010000000
VERSION     DAOMTQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15257_length_2189_cov_21.225398"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..628
                     /locus_tag="PK518_15790"
     CDS             350..628
                     /locus_tag="PK518_15790"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRM50419.1"
                     /translation="MARSLYLVAYDVCNPRRLARVTRYFHSYRVAGQKSVPEIWVTPA
                     ELAHIQADLRRLLQEGEDRLQLIALDPRMTPRCMGQAESFKATHFCVT"
     gene            668..1693
                     /gene="cas1"
                     /locus_tag="PK518_15795"
     CDS             668..1693
                     /gene="cas1"
                     /locus_tag="PK518_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HRM50420.1"
                     /translation="MSTLFVDRRDIALEHDSGALIIRDGGERIATVPLAPITRVVLRG
                     NAQLTASLLATLGERGIGVLVLTGRQGKPSLFFGRPHNDARLRVEQTRRSLQDDFCLA
                     YAQRLVQRKLERQCQWIDAAREHYPAQRYPLSQALRQLQEQLPQIAHTRRLDSLRGLE
                     GAAASGYFAGLRAVVPASLGFHERNRRPPRDPFNALLSLTYTLAHAEAAFTLHAAGLD
                     PCIGYYHQLSHSRESLACDIMETIRPLADRLCLKLVAQQVLTAEHFSQSDAGCLLGKA
                     GRSRYYGAYEEHAQPLRHAVQQEVRSLRQRIAPDMPEFECTRPDYDGDIYGALADSSA
                     EAAEATP"
     gene            1690..1989
                     /gene="cas2"
                     /locus_tag="PK518_15800"
     CDS             1690..1989
                     /gene="cas2"
                     /locus_tag="PK518_15800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRM50421.1"
                     /translation="MKETIICYDITCPRRLARIHRLLKKQALPLQYSVFLFTGTALQL
                     DRCLAQLERLMDKRHDDIRAYPLPQRGLRWSLGKATLPEGIHLGDLPAPWNGVIV"
BASE COUNT          438 a          829 c          605 g          317 t
ORIGIN      
        1 ggtgcatagg caaccgcaca tctactttta atagcgcctc acgcaataca gatacgggtt
       61 aacggctgtt ttccctctcc ccttgcggga gagggttggg gagaggggga gaaacgcctg
      121 caccaaccgc tttggcaagc ttgcccgccc accgccaggc aagcacgcgc aacacaatcc
      181 tttcatcgcc accccaccgg cacattcttg aaaggacacc ccatgaccat caactggcct
      241 tccgtggccg ccaaggcagc agcagcagcg ctcgtcgccg ccgctgcagc gctggccgaa
      301 gccctcagcg aaaagcgctg agcccaccac ccaaggctgc acccacccca tggcccgcag
      361 cctctacctc gtcgcctacg acgtatgcaa ccccaggcgg ctggcccggg tcacgcgcta
      421 ctttcacagc taccgcgtgg cggggcaaaa gtcagtgccc gaaatctggg tcacgcccgc
      481 cgagctggcg cacatccagg ccgacctgcg ccgcctgctg caagagggcg aagaccggtt
      541 gcaacttatc gccctcgatc cgcgcatgac gccgcgctgc atgggccagg ccgaaagctt
      601 caaggccacg cacttttgcg tgacctgaga gccgaacaac accgctaaac atcgccaaag
      661 gacagacatg agcaccctct tcgttgaccg ccgcgacatt gcactggagc acgacagcgg
      721 cgccctcatc attcgcgacg gcggcgagcg tatcgccacc gtgcccctgg cgcccattac
      781 ccgcgtcgta ctgcgcggca atgcccagct cacggccagt ctgctcgcca cattgggcga
      841 gcgcggcatt ggcgtgctgg tgctcaccgg ccgccagggc aagcccagcc tgttctttgg
      901 ccgcccgcac aacgacgcac gcctgcgcgt cgagcaaacc cggcgcagcc tgcaagacga
      961 cttttgcctg gcgtatgcgc aacgcctggt gcagcgcaag ctcgaacgcc aatgccaatg
     1021 gatcgatgca gcgcgcgagc actaccccgc ccagcgctac cccttgagcc aggcgctgcg
     1081 ccagctgcaa gaacagctgc cgcaaatcgc ccacacccgg cgcctggaca gcctgcgcgg
     1141 cctggagggc gccgccgcca gcggctactt tgccggcctg cgcgccgtgg tgcccgccag
     1201 cctgggcttt catgaacgca accgccgccc gccgcgcgac ccgttcaacg ccctgctgtc
     1261 gctcacctac accctggcgc acgccgaagc cgcctttacc ctgcacgccg ccgggctcga
     1321 cccctgcatc ggctactacc accaactgag ccactcgcgc gaatcgctgg cctgcgacat
     1381 catggaaacc atccgccccc tggccgaccg cctgtgcctg aagctggtgg cacagcaggt
     1441 gctgacggcc gaacacttca gccaaagcga cgccggctgc ctgctgggca aggccgggcg
     1501 cagccgctac tacggcgcct acgaagaaca cgcccaaccg ctgcgccacg ccgtgcaaca
     1561 agaagtcaga tccctgcgcc agcgcattgc gcccgacatg ccggaatttg aatgcacccg
     1621 ccccgactac gacggcgaca tctacggcgc gctggccgac agcagcgccg aagccgcgga
     1681 ggccacgcca tgaaagaaac catcatctgc tacgacatca cctgcccgcg ccgcctggcg
     1741 cgcatacacc gactgctgaa gaaacaggcg ctgccattgc aatactcggt gttcctgttc
     1801 accggaacgg ccttgcaact cgaccgctgc ctggcgcaac tggagcgcct catggacaag
     1861 cgccacgacg acatccgcgc ctaccccctg ccacaacgcg gcctgcgctg gagcctgggc
     1921 aaagccaccc tgcccgaagg catccacctg ggcgaccttc cagccccgtg gaacggcgtc
     1981 atcgtgtagc cccccgccga gtcgcagggg ccgacattta caatagacaa cgtcgaagcc
     2041 accttgcccc atgcccagcc cgcaccctgg aaagacatct cgggccccaa cgcccaacgt
     2101 ccataaggca ctctgcaagt gcttgtcagg caagggtttt ttcgaccttg gagctccaag
     2161 ccagccctga attcaaaggg attaagaca
//
LOCUS       DAOMTQ010000280         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_15374_length_2178_cov_23.887423, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000280 DAOMTQ010000000
VERSION     DAOMTQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15374_length_2178_cov_23.887423"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PK518_15805"
     CDS             complement(<1..584)
                     /locus_tag="PK518_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRM50422.1"
                     /translation="MVTKKPKGLGRGLEALLGPKVAEPAEQAQAAAAGLPSALALDQL
                     VPGMYQPRTRMDEGALYELAESIKAQGVMQPILVRRLQEGEHAGKYEIIAGERRFRAS
                     RLAGLNEVPVLVRDVPNESAAAMALIENIQREDLNPLEEAQGLQRLVKEFGLTHEQAA
                     QAVGRSRSAASNLLRLLNLADPVQTMLMAGDIDMG"
     gene            complement(609..1253)
                     /locus_tag="PK518_15810"
     CDS             complement(609..1253)
                     /locus_tag="PK518_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50423.1"
                     /translation="MARHNQPQRKVVTRFALIAIGGFIVLMLAWVKASPWLSYPVAVL
                     SHVALEQSTPMWIRSVDKQPGKITVDTSVEIVVPDSGGRRAEVLLEANPGRYAFGLPI
                     LGALLFAAWAVRRAPGRLWRLGLGYVLLLPFQSFSLVMYLLMELASASQFNIRTLRIE
                     QWQLEAIVYGYQFGVLVVPTLVPVLVWLLLDGKFFSAFAHDWKQSTRRAVWPSA"
     gene            complement(1295..1891)
                     /gene="xrtH"
                     /locus_tag="PK518_15815"
     CDS             complement(1295..1891)
                     /gene="xrtH"
                     /locus_tag="PK518_15815"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HRM50424.1"
                     /translation="MLRFFLIFLAWQMSLFGLNMLGWVQQHLVLPWTALLARVCAHLV
                     LWFDTTAAAAGKVLWNTVSGFGVSIEPGCNGIEACIVLFAAIMAYPASWRHKVIGIVA
                     GFAAVQGVNVLRVISLFYLGQWRTDVFNFAHEYLWQGLIMLDVLVVWLLWVRVVVRSR
                     PEESDPPEQPPAPPVLPVAPRGNPGKGVVSSSLDLSPR"
     gene            complement(1891..>2178)
                     /locus_tag="PK518_15820"
     CDS             complement(1891..>2178)
                     /locus_tag="PK518_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM50425.1"
                     /translation="DVERPDLATQIRGWAPIALQALPFPAVLVGSHNDPYCSFERAGA
                     LAADWGARLVDGGALGHINAESGLGAWPQGQALMRQLLAPDLEQEQQKEQG"
BASE COUNT          412 a          754 c          667 g          345 t
ORIGIN      
        1 cccatgtcga tgtcgccggc catgagcatg gtctgcaccg gatcggccag gttcagcagg
       61 cgcagcaggt tgctggcggc gctgcgcgag cggcccacgg cctgggccgc ctgctcgtgc
      121 gtgagaccaa attccttgac caggcgttgc aggccctggg cctcttccag cgggttcagg
      181 tcctcgcgct ggatgttctc gatcagcgcc atggcggcgg cagactcgtt gggaacgtcg
      241 cgcaccagca ccggcacctc gttcaggcca gccaggcgtg aggcacgaaa acgccgctcg
      301 ccggcgatga tttcgtactt gccggcatgc tcgccctcct gcaggcggcg caccagaatg
      361 ggctgcatca ctccttgggc cttgatgctc tcggccagct cgtacagtgc gccctcatcc
      421 atgcgcgtgc gcggctggta catgccgggc acgagttgat ccagcgccag cgcgctgggc
      481 aggccggcgg cggccgcctg ggcctgctcg gccggttctg cgaccttggg gccgagcagg
      541 gcttcgaggc cgcggcccag gcccttgggt ttttttgtca ccataagtga tgaagctttc
      601 tatcaaatct aggccgatgg ccacaccgcg cggcgtgtgg actgtttcca gtcatgcgca
      661 aatgcgctga aaaacttgcc atcgagcaac aaccacacca gcaccggcac cagcgttggc
      721 acgaccagca cgccaaactg gtaaccgtac acgatggcct ccagctgcca ctgctctatt
      781 ctgagcgtgc ggatattgaa ttggctggcg ctcgccaact ccatcagcaa atacatcacc
      841 aggctgaaac tttggaatgg cagcagcagc acatagccca ggcccaggcg ccagagccgg
      901 cccggcgcgc gcctgaccgc ccaggcggca aacagcagcg cacccagtat cggcagaccg
      961 aacgcatagc gccccgggtt ggcctccagc agaacctcgg cgcggcgccc accggagtcc
     1021 ggcacgacga tctcgaccga ggtatccacc gtaatcttgc cgggctgctt gtccacgctg
     1081 cgtatccaca tgggcgtgga ttgttccagt gccacgtgtg aaagcaccgc caccgggtac
     1141 gacagccagg gcgacgcctt gacccacgcc agcatcagca cgatgaagcc gccgatggcg
     1201 atcaacgcaa agcgcgtgac caccttgcgc tggggctggt tgtgacgtgc catgccttgt
     1261 tgtgcgttgt catccgccat caaatgcctc tccatcagcg cggcgacagg tccagcgaac
     1321 tgctgaccac gcccttgccg gggttgccgc gtggcgccac gggcagtacc ggcggcgctg
     1381 gcggctgttc tggcgggtca gactcttcgg gccggcttcg gaccaccacg cgcacccaca
     1441 gcagccacac caccagcacg tcgagcatga tcaggccctg ccagagatat tcgtgggcaa
     1501 agttgaaaac gtcggtgcgc cactgcccca ggtaaaacag gctgatcacg cgcaggacat
     1561 tcaccccctg caccgcggca aagccggcaa cgatgcctat caccttgtgc cgccaggacg
     1621 ccgggtaggc catgatggcg gcaaacagca cgatgcaggc ctcaatgcca ttgcagcccg
     1681 gctctatcga gacgccaaag ccggagaccg tgttccagag caccttgcca gccgccgcag
     1741 cagtggtgtc gaaccacagc accagatggg cacaaacgcg cgccagcaag gccgtccacg
     1801 gcaacaccag gtgctgctgt acccagccaa gcatgttcag gccgaagagc gacatctgcc
     1861 aggccaggaa gatcagaaag aaacgcagca tcagccctgc tccttctgct gctcttgctc
     1921 caggtctggc gccagcaact gccgcatcag cgcctgcccc tggggccagg cgcccaggcc
     1981 cgattcggca ttgatatgcc cgagcgcgcc gccatcgaca aggcgcgccc cccagtctgc
     2041 cgccagggcg ccggcgcgtt cgaagctgca atagggatcg ttgtggctgc ccaccagcac
     2101 cgcaggaaac ggcaacgcct ggagcgcaat gggcgcccag ccgcggatct gcgtggccag
     2161 gtcgggccgc tccacatc
//
LOCUS       DAOMTQ010000281         2171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_15448_length_2171_cov_28.551040, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000281 DAOMTQ010000000
VERSION     DAOMTQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15448_length_2171_cov_28.551040"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..983)
                     /locus_tag="PK518_15825"
     CDS             complement(6..983)
                     /locus_tag="PK518_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRM50426.1"
                     /translation="MKKRTLIQAAAAAAASLAFSSPALAQDFPPAKPVTLVVGFAAGG
                     AADAAARLIAKKLGENIGQSVVVDNKGGAGGNIAHQQVAAGAADGTVLLLGSVGPLTI
                     APHLMKLPYDPFKDLAPITGGVYFPNVLVVHKAAGAKTLAEFVQLSKKKPGSVDFAST
                     GAGSASHLAGELFNQRAGIDMVHVPYKGGAPALQDLLGERVTSYFAAPPTAMPHVETG
                     KLIPLATTGLTRPAYLPNIPTVAESGYPGFQALNWYSFMASGKTPAPLLERWNQEIVK
                     VLNDPGVKDALNKHGLSPHPTTRAEFASFMKKEYDQWGAIVRERKITGQ"
     gene            complement(1071..>2171)
                     /gene="tcuB"
                     /locus_tag="PK518_15830"
     CDS             complement(1071..>2171)
                     /gene="tcuB"
                     /locus_tag="PK518_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HRM50427.1"
                     /translation="MQICNACRYCEGFCAVFPAMTRRLEFLKADVHYLANLCHNCGAC
                     LHACQYAPPHEFAVNVPQAMARVRGQTYHDYAWPLALGQLYKRNGLTLSLALVAALTL
                     FLALAAALKGGWGALWGVDLGANFYHLFPHNLMVGLFAPIFLFAVLALAMGVRGFWRD
                     VKPATSGADVSDAAASEAAHDVLRLKYLDGGHGDGCNNEDDAFSLTRRRCHHLTFYGF
                     LLCFAATSLATVYHYAFGWVAPYDLPSLPKILGAVGGASLMLGTAGLWRLNLRRHPLQ
                     GDAAQKPMDLGFIALLFLVAASGLALWLVRITPAATPALALLLCLHLGAVMALFATMP
                     YGKFAHGIFRTAALLRHNTEKRRPNPIGLGSD"
BASE COUNT          342 a          699 c          786 g          344 t
ORIGIN      
        1 cggcctcact gccccgtgat cttgcgctcg cgcacgatgg cgccccactg gtcgtattcc
       61 ttcttcatga agctggcgaa ctcggcgcgc gtggtcgggt ggggcgacag gccgtgcttg
      121 ttcagcgcgt ccttcacgcc ggggtcgttg agcaccttga cgatctcctg gttccagcgt
      181 tcgagcagcg gcgcgggcgt cttgcccgag gccatgaacg aataccagtt cagcgcctga
      241 aagccggggt agcccgactc ggccacggtg ggtatgttgg gcaggtaggc cggccgcgtc
      301 agccccgtgg tggccagcgg gatcagtttg ccggtctcga catgcggcat ggccgtgggc
      361 ggggcggcaa agtagctggt cacgcgctcg cccagcaggt cttgcagcgc gggcgcgccg
      421 cctttgtagg gaacatgcac catgtcgatg ccggcgcgct ggttgaacag ttcgcccgcc
      481 aggtgcgatg ccgagcccgc gccggtagag gcaaagtcca cgctgccggg cttctttttg
      541 gacagctgga cgaactcggc cagcgtcttc gcgccggcgg ccttgtgcac caccagcacg
      601 ttggggaaat acacgccacc ggtgatgggc gccaggtcct tgaacgggtc gtagggcagc
      661 ttcatgaggt gcggcgcaat cgtcagcggc cctaccgagc ccagcagcag cacggtgccg
      721 tcggcagcgc ccgccgccac ctgctggtgg gcgatgttgc ccccggcgcc gcccttgttg
      781 tccaccacca cgctctggcc gatgttctcg cccagcttct tggcgatgag ccgcgccgcc
      841 gcatcggccg cgccgccggc ggcaaagccc accaccagcg tcactggctt ggccggcgga
      901 aagtcctgcg ccagcgcagg gctggaaaag gccagcgagg cggccgctgc ggcggcggcc
      961 tggatcaggg tgcgtttttt catcgtgagg ctccttggcg gacgttttaa gtgaaatagg
     1021 gctgcagccc ttgtggggca agcgctgaca gctattgttt ttgtggtgtt tcagtcgctg
     1081 cccaggccta tggggttggg ccggcgcttt tcggtgttgt ggcgcagcag cgccgcggtg
     1141 cggaagatgc cgtgggcaaa cttgccgtag ggcatggtgg caaacagcgc catcaccgcg
     1201 cccaggtgca ggcacagcag cagggccagg gcgggcgtgg ccgccggggt aatgcgcacc
     1261 agccacagcg ccaggccgct ggcggccacc aggaacagca gggcgatgaa gcccaggtcc
     1321 atgggtttct gggcggcgtc gccctgcagc gggtggcggc gcaggttcag gcgccacagg
     1381 ccggccgtgc ccagcatcag gctggcgccg cccaccgcac ccaggatctt ggggaggctg
     1441 ggcaggtcat acggcgccac ccagccaaag gcatagtggt acaccgtggc cagcgaggtg
     1501 gcggcaaagc acagcaggaa gccgtagaag gtcaggtggt ggcagcgccg gcgcgtcagg
     1561 ctgaaggcgt cgtcctcgtt gttgcaaccg tcgccatggc cgccgtccag gtatttcagg
     1621 cgcagcacgt cgtgcgcggc ctcgctggcg gcggcgtcac tcacgtcggc gccgctggtg
     1681 gcgggcttga cgtcgcgcca gaagccgcgc acgcccatgg ccagcgccag caccgcaaac
     1741 aggaagatgg gcgcaaacag gcccaccatc aggttgtgcg ggaacaggtg gtaaaagttg
     1801 gcgcccaggt ccacgcccca cagcgcaccc cagccgccct tgagcgcggc ggccagcgcc
     1861 aggaacagcg tcagcgcggc caccagggcc agcgacagcg tcaggccatt gcgcttgtag
     1921 agttgtccga gcgcgagcgg ccaggcgtag tcgtggtagg tctggccgcg cacccgggcc
     1981 atggcctgcg gcacgttgac ggcaaactcg tgcggcggcg cgtactggca ggcgtgcagg
     2041 caggcgccgc agttgtggca caggttggcc aggtagtgca cgtcggcctt gaggaattcg
     2101 agccggcgcg tcatggccgg gaacacggcg caaaagccct cgcagtagcg gcaggcgttg
     2161 cagatctgca t
//
LOCUS       DAOMTQ010000282         2145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_15720_length_2145_cov_30.233971, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000282 DAOMTQ010000000
VERSION     DAOMTQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15720_length_2145_cov_30.233971"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..851
                     /locus_tag="PK518_15835"
     CDS             114..851
                     /locus_tag="PK518_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM50428.1"
                     /translation="MIELKNVSKWYGSFQVLNDCSTNIQKGEVVVVCGPSGSGKSTLI
                     KTINALEPIQKGEIYVDGTPIHDPKTNLPKLRSRVGMVFQHFELFPHLSVTENLTIAQ
                     IKVLGRSPDDAKARGLKMLDRVGLMAHKDKFPGQLSGGQQQRVAIARALSMDPIVMLF
                     DEPTSALDPEMVGEVLDVMMGLAHEGMTMMCVTHEMGFAKKVSDRVIFMDVGGKILED
                     CSKEEFFGNPDARQPRTKDFLNKILQH"
     gene            complement(981..1652)
                     /locus_tag="PK518_15840"
     CDS             complement(981..1652)
                     /locus_tag="PK518_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRM50429.1"
                     /translation="MTLIGVGAATNATQNGRVSLGHIGSGYTFPGMYGQAVYTTPNMG
                     GFSVDLGLMSPTNNLSTAKKDNLQFQARATYAGQGFKAWVGAKTQKYDGVGGFTMNAG
                     EVGGSVSAGAFGLLANYQGGKGIGILADGDQAGVKGNNLLLQGTMQAAQNVKLGLGYG
                     RSKNDSAFTGSDLRSNENLTAGVYYNLTKSVTLVGEIGQTRSKGFSGASAKQNSVAIG
                     GILFF"
     gene            complement(1649..2107)
                     /locus_tag="PK518_15845"
     CDS             complement(1649..2107)
                     /locus_tag="PK518_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50430.1"
                     /translation="MDRKLIAAAALAAFALPAAQAVDIQAGDWTVSIGGNVNAFYTQT
                     DCKQPAGTVTGTALGDAGLACAGKEKTTVIGNGLLPSYLSVGAKSRQGGFDVSANVGI
                     GVSVATGSSIAQNNDVDVRQAYLSFGNDGMARSSWGATTGCSVFIPSSAT"
BASE COUNT          395 a          643 c          711 g          396 t
ORIGIN      
        1 gcgctgcggg aagggggacg ccaccagcgc ggcggggcgg cccttgcgcg gtggccctgg
       61 cctgggccgc gcttcttgca cgcgtagcgg ggcataagag attggagtga aaaatgattg
      121 aactgaagaa cgtttccaag tggtacggca gcttccaggt gctcaatgac tgctccacca
      181 acatccagaa gggtgaggtg gtggtggtct gcggcccctc gggatcgggc aaatccacgc
      241 tgatcaagac catcaacgca ctcgaaccca ttcaaaaggg cgagatttat gtggacggca
      301 cgcccatcca cgaccccaag accaatctgc ccaagctgcg ctcgcgcgtg ggcatggtgt
      361 tccagcattt cgagctgttt ccgcacctgt cggtgaccga gaacctgacc atcgcccaga
      421 tcaaggtgct ggggcgcagc ccggacgatg ccaaggcgcg cggcctgaag atgctcgacc
      481 gcgtgggcct gatggcgcac aaggacaagt tcccgggcca gctctcgggc ggccagcagc
      541 agcgcgtggc gattgcgcgc gcgctgtcca tggaccccat cgtcatgctg ttcgatgagc
      601 ccaccagcgc gctcgacccc gagatggtgg gcgaggtgct ggacgtgatg atgggcctgg
      661 cccacgaggg catgaccatg atgtgcgtga cgcacgagat gggttttgcc aagaaggtca
      721 gcgaccgcgt catcttcatg gacgtgggcg gcaagattct ggaagactgc agcaaggaag
      781 agttctttgg caaccctgac gcgcgccagc cgcgcaccaa ggacttcctg aacaagatcc
      841 tgcagcattg atgggcctgg tggctcgcag gcctgccggg gcagggacgc gaggctgtgc
      901 cggggcgaaa aaaaggcgac cgaccgtccc cgcagcagcc ggtcgccaac cctgaagaac
      961 aaaaaaccgt tgcgtgtcga tcagaagaag aggatgccgc cgatagcgac gctgttctgc
     1021 ttggcgctgg cgccgctgaa gcccttggat cgggtctgac cgatttcacc caccagggtc
     1081 acgctcttgg tcaggttgta gtagacgcca gcggtcaggt tttcattcga gcgcagatca
     1141 gagccggtga aggcgctgtc gttcttgctc ctgccgtaac ccaggcccag cttcacgttc
     1201 tgggcggcct gcatggttcc ttgcagcaga aggttgttgc ccttcacgcc ggcctggtcg
     1261 ccgtcggcca gaatgccgat gcccttgccg ccctggtagt tggccagcag gccgaacgcg
     1321 ccggcgctga cggatccgcc gacttcgccg gcgttcatgg tgaagccgcc cacgccgtca
     1381 tatttctggg tcttggcgcc cacccaggcc ttgaacccct ggccagcgta ggtggcccgg
     1441 gcctggaact gcaggttgtc cttcttcgcc gtgctcaggt tgttggtcgg gctcatcagg
     1501 cccagatcca cactgaaacc acccatgttc ggggtggtgt aaacggcttg cccgtacatg
     1561 ccggggaagg tgtagccgga gccgatatgg cccagcgaga cgcggccatt ttgcgttgcg
     1621 ttggtggccg caccgacacc gatgagggtc atgtcgctga tgatggaatg aaaaccgaac
     1681 agcccgtagt cgcgccccag cttgaccgtg ccatgccgtc attgccaaag ctcaggtagg
     1741 cctggcgaac gtccacgtca ttgttctgcg cgatggagct gccggtggcg acggagacgc
     1801 ctatgcccac attggcggac acatcaaagc cgccctgcct ggacttggcg ccgacggaga
     1861 ggtaagacgg cagcaggccg ttgccgatca cggtggtctt ttccttgccg gcgcaggcca
     1921 ggccggcatc acccagggcg gtgccagtca cggtgcccgc gggttgcttg caatcggtct
     1981 gggtgtagaa ggcattgacg ttgccgccga tggacacagt ccagtcgccg gcctggatgt
     2041 caacggcctg ggctgcgggg agggcgaagg ctgcgagtgc agcggctgca atgagttttc
     2101 tgtccatcta tgtctccgtg gtgataaatg acgtggtacg tgatc
//
LOCUS       DAOMTQ010000283         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_16341_length_2090_cov_24.620639, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000283 DAOMTQ010000000
VERSION     DAOMTQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16341_length_2090_cov_24.620639"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PK518_15850"
     CDS             complement(<1..158)
                     /locus_tag="PK518_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRM50431.1"
                     /translation="MTKQRVVVGLSGGVDSAVTAYLLKQQGHEVVGIFMKNWEDDDDS
                     EYCSSNID"
     gene            225..1196
                     /locus_tag="PK518_15855"
     CDS             225..1196
                     /locus_tag="PK518_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM50432.1"
                     /translation="MELRQLRYFVRVVELGSMGRAAIDLNLVQSALSQQIARLEGELA
                     TRLLHRTPRGVAPTEAGAAFFREARLTLRHAEQAARAAQQARLSGTVTMGLAPTTSAV
                     LGLPLMQAMRARYPDVRLHMVEGMSGHLSAMLHARDLDLAVLFDSRLHQAAQAGRGPR
                     WQVQALLEEDLFLMRGAAAARLPAQVTLADLQDEPLILPTGPHGLRSTLDTAFASAGI
                     APRVVLEVDSLAMVMAAVDAGLGATVQPWAALGRYPDADTRFQCALLTGAPVRRVNLL
                     CNLPDEELSPAALAARVVLLDCVRALVAEGRWSGTRLMAEPPATPAA"
     gene            1333..>2090
                     /gene="tcuA"
                     /locus_tag="PK518_15860"
     CDS             1333..>2090
                     /gene="tcuA"
                     /locus_tag="PK518_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HRM50433.1"
                     /translation="MQSPTMDADVLVIGGGNAALCAALMAREAGARVLLLESAPRDWR
                     GGNSAHTRNLRCMHDAPQDVLVDAYPEEEYWQDLLKVTGGITDEHLARLVIRHSSACR
                     PWMRRHGVRFQPALAGTLHVARTNAFFMGGGKALVNAYYRSAQALGVQVRYDSPVDRL
                     DIEDGQFQAAWVQGQRITAKTCVLAAGGFESNREWLREAWGQNSRGEWPADNFLIRGT
                     AFNRGVLLKHLLDDHGADRIGDPTQAHMVAIDARA"
BASE COUNT          290 a          752 c          730 g          318 t
ORIGIN      
        1 aagtcgatgt tggacgagca gtactcgctg tcgtcgtcat cctcccagtt cttcatgaag
       61 atgccgacca cctcgtggcc ctgttgtttc aatagatagg ccgtgacggc ggaatccacc
      121 ccgccggaga ggcccaccac cacccgttgt ttcgtcatag gtgtgcgatt atcccgctct
      181 accccggccg ggcggcccat ggggtcaaca tgaggcgccg gacgatggaa ctgcggcaac
      241 tgcgatattt cgtgcgtgtg gtggagctgg gctccatggg ccgcgccgcg atcgacctga
      301 acctggtgca gtcggccctg agccagcaga tcgcgcgcct ggagggcgag ctggccacgc
      361 gcctgctgca ccgcacgccg cgcggcgtgg cgcccaccga ggccggggcg gccttctttc
      421 gcgaggcccg gctgaccctg cgccatgccg agcaggccgc gcgcgcggcg cagcaggcgc
      481 ggctgtcggg cacggtcacc atgggcctgg cgcccaccac ctcggccgtg ctgggcctgc
      541 cgctgatgca ggccatgcgc gcacgctacc ccgatgtgcg gctgcacatg gtggagggca
      601 tgtcgggcca cctctcggcc atgctgcacg cgcgcgatct ggatctggcg gtgctgttcg
      661 actcgcgcct gcaccaggcc gcgcaggccg ggcgcggccc gcgctggcag gtgcaggcat
      721 tgctggagga agacctgttt ctgatgcgcg gcgcggccgc cgcccgcctg ccggcgcagg
      781 tgaccctggc cgacctgcag gacgagcccc tgatcctgcc caccggcccg cacggcctgc
      841 gcagcacgct ggacacggcc tttgccagcg ccggcattgc accgcgcgtg gtgctggagg
      901 tggattcgct ggccatggtg atggccgccg tcgatgccgg cctgggcgcc acggtgcagc
      961 cctgggccgc cctgggccgc tacccggatg cggacacgcg ctttcaatgc gcgctgctga
     1021 ccggcgcgcc ggtgcggcgg gtgaatctgc tgtgcaacct gcccgacgaa gagctgtcac
     1081 ccgcggcact ggccgcgcgg gtggtgctgc tggactgcgt gcgcgccctg gtggccgagg
     1141 ggcgctggtc cggcacccgc ctgatggccg agcccccggc cacgccggcc gcctgatcgg
     1201 cacgcgccgg tcatcagtga tcgtgatggc cccatgcccc cgccggcctg tgcgccgcgc
     1261 gggatgcggc ctacagtcgg cgcatcccat tcgatagaag cggcgcgcct cgacgtgcgc
     1321 cagaacatca ccatgcaatc tcccacgatg gacgccgatg tcctggtcat cggcggcggc
     1381 aacgccgcgc tgtgcgccgc gctgatggcg cgcgaggcgg gtgcgcgcgt gctgctgctc
     1441 gaatcggccc cgcgcgactg gcgcggcggc aactccgcgc acacgcgcaa cctgcgctgc
     1501 atgcacgacg caccgcagga cgtgctggtc gatgcctacc ccgaagaaga gtattggcag
     1561 gacttgctga aagtgaccgg cggcattacc gacgagcacc tggcacgcct ggtgatccgc
     1621 cactcgtcgg cctgccgccc ctggatgcgc cgccacggcg tgcgttttca gcccgcgctg
     1681 gccggcacgc tgcatgtggc gcgcaccaat gccttcttca tgggcggcgg caaggcgctg
     1741 gtcaacgcct actaccgcag cgcccaggcg ctgggcgtgc aggtgcgcta cgactcgccc
     1801 gtcgatcggc tggacatcga ggacgggcag ttccaggccg cctgggtcca gggccagcgc
     1861 atcacggcca agacctgcgt gctggcggcc ggcggctttg agtccaaccg cgaatggctg
     1921 cgcgaggcct ggggccagaa ttcgcgcggc gaatggccgg ccgacaattt tctgatccgc
     1981 ggcacggcct tcaaccgcgg cgtgctgctc aaacacctgc tcgatgacca cggcgccgac
     2041 cgcatcggcg accccacgca ggcgcacatg gtggccatcg acgcacgcgc
//
LOCUS       DAOMTQ010000284         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_16380_length_2086_cov_26.356475, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000284 DAOMTQ010000000
VERSION     DAOMTQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16380_length_2086_cov_26.356475"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="murA"
                     /locus_tag="PK518_15865"
     CDS             <1..968
                     /gene="murA"
                     /locus_tag="PK518_15865"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM50434.1"
                     /translation="ARFGEATVSLPGGCAIGSRPVDQHIKGLQAMGAQITVEHGYMIA
                     KLPPGHSRLRGARISTDMITVTGTENLLMAATLAEGETVLQNAAQEPEVTDLAEMLIK
                     MGAKIEGHGTSRISIQGVDRLHGCTHQVVADRIEAGTFLCAVAATGGDAFLRHARADH
                     LDAVIDKLLEAGVQVTAEDGGMRVRSPGGALLKAQSFRTTEYPGFPTDMQAQFMALNV
                     LAQGTSTVTETIFENRFMHVNELSRLGAHIAIDGREATVTGGAQLSGATVMATDLRAS
                     ASLVIAGLVASGETLVDRIYHLDRGYDRMEDKLRGLGADIERLST"
     gene            971..1612
                     /gene="hisG"
                     /locus_tag="PK518_15870"
     CDS             971..1612
                     /gene="hisG"
                     /locus_tag="PK518_15870"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRM50435.1"
                     /translation="MITLALSKGRIFDETLPLLAAAGITVTEDPEKSRKLILATNQPH
                     VRVVLVRASDVPTYVEYGGADLGVAGKDSLIEHGGQGLFRPLDLQIAKCRVSVAVRNG
                     FDYQEAVTQGSRLKVATKYTSIARDFFSAKGVHVDMIKLYGSMELAPLTGMADAIVDL
                     VSTGNTLKANDLVEVEHIMDISSHLVVNQAALKLKQAGLKPIIDAFASAVPQD"
     gene            1624..>2086
                     /locus_tag="PK518_15875"
     CDS             1624..>2086
                     /locus_tag="PK518_15875"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRM50436.1"
                     /translation="MQFVAAPARLSTASATFEADFAARLHWSAEQDAAIEERVAAILA
                     DVQTRGDAAVLEYTQRFDGVQAQSMAALELSQAELKAAYDSLPAEQKDALTAAAARVR
                     SYHEAQKKACGESWSYRDQDGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMN"
BASE COUNT          379 a          715 c          680 g          312 t
ORIGIN      
        1 tggcgcgctt tggcgaggcc acggtgtcgc tgcccggcgg ctgcgccata ggctcgcgcc
       61 cggtggacca gcacatcaag ggcctacagg ccatgggcgc gcagatcacg gtcgagcatg
      121 gctacatgat cgccaagctg ccgccggggc acagccgcct caggggcgcg cgcatctcca
      181 ccgacatgat caccgtcacc ggcaccgaga acctgctgat ggcggccacg ctggccgagg
      241 gcgagacggt gctgcagaac gccgcccagg agcccgaggt gaccgacctg gccgaaatgc
      301 tgatcaagat gggcgcgaag atcgagggcc atggcaccag ccgcatcagc atccagggcg
      361 tggatcggct gcacggctgc acgcaccagg tggtggccga ccgcatcgag gccggcacct
      421 ttttgtgcgc cgtggccgcc accggcggcg acgccttttt gcgccacgcc cgtgccgacc
      481 atctggatgc cgtgatcgac aagctgctgg aggccggcgt gcaggtcacc gccgaggacg
      541 gcggcatgcg cgtgcgcagc cctggcggcg cgctgctgaa ggcgcagagc tttcgcacca
      601 ccgaataccc cggctttccg accgacatgc aggcgcagtt catggccctg aacgtgctgg
      661 cccagggcac gtccacggtc accgagacca tctttgaaaa ccgcttcatg catgtcaatg
      721 agctctcgcg cctgggcgcg catatcgcca ttgacgggcg cgaggccacg gtgaccggcg
      781 gcgcccagct gtctggcgcc accgtcatgg ccaccgacct gcgcgcctcg gccagcctgg
      841 tgattgccgg cctggtggcc agcggcgaga ccctggttga ccgcatctac cacctggacc
      901 gtggctacga ccgcatggag gacaagctgc gcggcctggg tgccgacatc gaaagactct
      961 ccacatgagc atgatcaccc tggccctgtc caagggccgc atctttgacg agaccctgcc
     1021 gctgctggcc gccgccggca tcacagtgac ggaagacccg gagaagtcgc gcaagctcat
     1081 tctggccacc aaccagcccc atgtgcgcgt ggtgctggtg cgcgccagcg acgtgccgac
     1141 ctacgtcgaa tacggcggcg ccgacctggg cgtggccggc aaggactcgc tgatcgagca
     1201 tggcggccag gggctgtttc gcccgctgga cctgcagatt gccaagtgcc gcgtcagcgt
     1261 ggcggtgcgc aatggctttg attaccagga ggccgtcacc cagggctcgc gcctgaaggt
     1321 ggccaccaag tacaccagca tcgcgcgcga cttcttctcg gccaagggcg tgcatgtgga
     1381 catgatcaag ctctacggca gcatggagct ggcgccgctg accggcatgg cggacgccat
     1441 cgtcgatctg gtctccaccg gcaacacgct caaggcgaac gacctggtcg aggtcgagca
     1501 catcatggac atcagcagcc acctggtggt gaaccaggcc gcgctgaaat taaagcaagc
     1561 cggactcaag cccatcatcg acgcctttgc ctctgccgtg ccccaggatt gatcaggttt
     1621 actatgcaat tcgtagctgc tcccgcacgc ttgtcaaccg cttcagccac ttttgaggct
     1681 gattttgcag cccgcctgca ctggtcggcc gagcaggacg ccgccatcga ggagcgtgtc
     1741 gccgccatcc tggccgacgt gcagacacgc ggcgacgcgg cggtgctcga atacacccag
     1801 cgctttgacg gcgtgcaggc acagagcatg gccgcgctgg agctgagcca ggccgaattg
     1861 aaagccgcct acgacagcct gccggccgag caaaaagacg cgctgacggc cgccgccgcc
     1921 cgcgtgcgca gctaccacga ggcgcagaaa aaagcctgcg gcgagagctg gagctaccgc
     1981 gaccaggacg gcacgctgtt gggccagaag gtcaccccac tcgaccgcgt cggcatctac
     2041 gtgcccggcg gcaaggccgc ctaccccagc agcgtgctga tgaacg
//
LOCUS       DAOMTQ010000285         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_16681_length_2061_cov_24.844965, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000285 DAOMTQ010000000
VERSION     DAOMTQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_2061_cov_24.844965"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK518_15880"
     CDS             <1..351
                     /locus_tag="PK518_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HRM50437.1"
                     /translation="ASAGFEGAWARDLARDAGEPEPLVRTTLSRLARRGELHQVVKDL
                     FYPPPAMAQLAALARQVGQAHHGVVLAAQFRDMTGLGRKRAIQILEYFDRIGFTRRVA
                     DMHRLRGDCALFEV"
     gene            474..569
                     /locus_tag="PK518_15885"
     tRNA            474..569
                     /locus_tag="PK518_15885"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:508..510,aa:Sec,seq:tca)
     gene            complement(570..962)
                     /locus_tag="PK518_15890"
     CDS             complement(570..962)
                     /locus_tag="PK518_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRM50438.1"
                     /translation="MSFTEHDAAGIAILLWSADPGCPERLATPFFHAAAAAAMDMPVE
                     IYFSAASVRLLVPAVAAGLRASSRVEKTIADNLGEAVQLGARLFACSDALAAQGLAAP
                     DLMPGCERGGAVQFMARAMDLRWRALVF"
     gene            complement(959..1417)
                     /locus_tag="PK518_15895"
     CDS             complement(959..1417)
                     /locus_tag="PK518_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HRM50439.1"
                     /translation="MSALQLHFPPDLHYLLEHQVWARLDGDGLATVGITALGIALSGE
                     IYMCRPKRLGTALAQGATLAVVELAKSIVAVKTPVSGSIVAINPALEQRPGLVHTDPY
                     GAGWIAQLALTDWAADLGALVHGDAVGPAMAAHARAYGHELAAGGAGAAP"
     gene            1606..>2061
                     /gene="ssb"
                     /locus_tag="PK518_15900"
     CDS             1606..>2061
                     /gene="ssb"
                     /locus_tag="PK518_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM50440.1"
                     /translation="MASVNKVIIVGNLGRDPEMRTFPSGDQVANVRIATTDRWRDKNT
                     GENKEATEWHSVVFNGRLAEIVGQYLRKGSQVYVEGSLRTRKWTDQSGQERYTTEIRA
                     DTMQMLGSRQGMGGGQGGGAGGYDDGGYGDAGGGYEAPRRAAPPQQRPAA"
BASE COUNT          343 a          737 c          712 g          269 t
ORIGIN      
        1 gccagcgccg gcttcgaggg tgcctgggcg cgcgacctgg cgcgcgatgc cggcgagccc
       61 gagcccctgg tgcgcaccac gctgtcgcgc ctggcgcgcc gcggcgagct gcaccaggtg
      121 gtcaaggact tgttctaccc gccgcccgcc atggcgcaac tggccgccct ggcgcgccag
      181 gtgggccagg cgcaccacgg cgtggtattg gcggcacagt ttcgcgacat gacgggcctg
      241 gggcgcaagc gggcgataca aattctggaa tacttcgacc gcatcggctt cacgcgccgc
      301 gtcgcggaca tgcaccggct gcgcggcgac tgcgctttgt tcgaagtttg aactcgtagg
      361 agcggcttca gccgcgaatg gcgccgttcg cggctgaagc cgctcctacg gccggtgcac
      421 gcttcaaagg gcctagatca atattgacct gattggggcg gagcaatgac aacggaaggg
      481 caaccaccct ggtgggtggg ccgggcttca aacccggtgg gtggcgccaa gcgccgccgg
      541 gtgggttcga ctcccatgcc tttccgccat caaaaaacca gggcgcgcca gcgcagatcc
      601 atggcccgcg ccatgaactg caccgcaccg ccgcgctcgc agcccggcat caggtctggc
      661 gcggccaggc cctgggccgc cagcgcgtcg gagcaggcaa acagccgcgc ccccagctgc
      721 accgcctcgc ccaggttgtc ggcaatcgtc ttctccaccc ggctgctggc gcgcaggccg
      781 gccgccaccg ccggcaccag caggcgcacg ctggcggcgc tgaagtagat ctccaccggc
      841 atgtccatgg ccgccgccgc cgccgcgtga aagaacggcg tcgccagccg ctcggggcag
      901 ccagggtcgg ccgaccacag caggatggcg atgccggccg catcgtgctc ggtaaagctc
      961 atggcgccgc cccggcgccg cccgctgcca gctcatggcc gtaggcacgg gcatgcgcgg
     1021 ccatggcagg gccgaccgcg tcgccatgca ccagcgcgcc caggtcggcg gcccagtcgg
     1081 tcagcgccag ttgcgcgatc cagcccgcgc cataggggtc ggtatgcacc aagccggggc
     1141 gctgctccag ggctgggttg atggcgacga tgctgcccga caccggggtc ttcaccgcga
     1201 cgatggactt ggccagctcc accaccgcca gcgtcgcccc ctgcgccagg gccgtgccca
     1261 ggcgcttggg gcggcacatg tagatctcgc ccgacagggc aatgcccagt gccgtgatgc
     1321 ccaccgtggc caggccgtcg ccgtccagcc gcgcccagac ctggtgctcc agcaggtaat
     1381 gcagatcggg cggaaagtgc agttgcaagg cgctcatgga cggggtgatg gcgttggggc
     1441 gaggggcatg atagccaggg gccgcaattg ccggaatacg ggctgcaaca accgcgccgc
     1501 tgtgcgctat gctgcgcacc tgcggcctcg gaggaggccg cgcattcaag ctcatctggc
     1561 ccggaatcct cccccctgcc gtccgggcta ccgcaaagga attccatggc atccgtgaac
     1621 aaagtcatca tcgtcggcaa cctggggcgc gaccccgaga tgcgcacctt ccccagcggc
     1681 gaccaggtcg ccaacgtgcg catcgccacc accgatcgct ggcgcgacaa gaacaccggc
     1741 gagaacaagg aagccaccga gtggcacagc gtggtcttca acggccgcct ggccgagatc
     1801 gtcggccagt acctgaggaa gggcagccag gtctatgtgg aaggcagcct gcgcacgcgc
     1861 aagtggaccg accagagcgg ccaggagcgc tacaccaccg aaatccgcgc cgacaccatg
     1921 caaatgctgg gtagccgcca aggcatgggc ggcggccagg gcggcggcgc cggtggctat
     1981 gacgacggtg gctacggcga cgccggcggc ggctacgagg ccccgcgccg cgccgcgccc
     2041 ccgcagcaac gccccgcggc t
//
LOCUS       DAOMTQ010000286         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_16747_length_2056_cov_28.447276, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000286 DAOMTQ010000000
VERSION     DAOMTQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16747_length_2056_cov_28.447276"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1049)
                     /locus_tag="PK518_15905"
     CDS             complement(261..1049)
                     /locus_tag="PK518_15905"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRM50441.1"
                     /translation="MSDEADLLQVERDARGVVTLTLNDPKRFNALGHEMLAALQQALA
                     DVARDEGARVVVLAAAGKAFCAGHNLKDMAQHPDLAWYQDLFGRCSRVMLAIHKLPVP
                     VIARVQGMATAAGCQLVAQCDLAVASEAASFATSGIHYGLFCATPSVPLVRNVPTKRA
                     MEMLLTGDFIDARTALEQGLVNRVVAPGLLDVEVELLVQSIVEKPRAAVAMGKALVYQ
                     QRELGLEAAYQLAGQAMACNMMDAAAQEGARAFAEKRQPAWKPG"
     gene            complement(1064..1969)
                     /locus_tag="PK518_15910"
     CDS             complement(1064..1969)
                     /locus_tag="PK518_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRM50442.1"
                     /translation="MGPGHPECPARLDAIEDRLLVSGVSAALDHREAPLASLADIELA
                     HDRMHVAALRGLSLRLVEEEEAGGSPYFQLDTDTAMNRHTWTAALRAAGAAVDAVDAI
                     MAGELENAFCSVRPPGHHAEHKKAMGFCFFNNVAVAAKYALQRHNLKRVAVIDFDVHH
                     GNGTEDILAGDERALMVSIFQHPFYPYSGDQDPAPNMLNVPVPAYTKGMDVREIVEMV
                     WMPRLEAFKPEMIFVSAGFDAHRDDDMGQLGLTEQDYTWITMRIKDVARRFANKRIVS
                     CLEGGYVMGPLARSVEAHVRVLADL"
BASE COUNT          345 a          687 c          648 g          376 t
ORIGIN      
        1 cgtccgattt cacgcggacc ttcacgttgt agtccaccac gcgtttgacg gggaccaaga
       61 ccttcattgc tgctgctcct tcaattgttg attgacgtgt acgtaaaccg gatcattcta
      121 ttgcgcactg cagcattttg accatcggca atacctgcaa gcccgcactt tctaaaaaaa
      181 gaacgatcgt gcttttaaat gattatgcca gggtaccgat gcgcagccaa gcggctgcga
      241 cacctttgcc accgggacga tcagcccggc ttccaggccg gctgacgttt ctcggcaaag
      301 gcgcgcgcgc cctcctgggc cgccgcgtcc atcatgttgc aggccatggc ctgaccggcc
      361 agctgatagg cggcctccag tcccagttcg cgctgttgat agaccagggc cttgcccatg
      421 gcgacggccg cgcggggctt ttccacgatg gactgcacca gcagctcgac ctcgacgtcc
      481 agcagcccag gcgccacgac gcggttcacc agcccctgct ccagggccgt gcgcgcgtcg
      541 atgaaatcgc ccgtgagcag catttccatg gcccgcttgg tcggcacgtt gcgcaccagg
      601 ggcacgctgg gcgtggcgca gaacaggccg tagtggatgc cgctggtggc aaagctggcg
      661 gcctcgctgg ccacggccag gtcgcattgc gccaccagct ggcagccggc cgccgtggcc
      721 atgccctgca cgcgcgcgat caccggcacg ggcagcttgt ggatggccag catcacgcgg
      781 ctgcagcgcc caaacaagtc ctggtaccag gccagatccg ggtgctgcgc catgtccttg
      841 aggttgtggc cggcgcagaa ggccttgccg gccgcggcca gcaccaccac gcgcgcgccc
      901 tcgtcccgcg ccacatcggc cagcgcctgc tgcaacgcgg ccagcatctc gtggcccagg
      961 gcgttgaagc gcttgggatc attcagggtc agcgtgacca cgccgcgcgc atcgcgctcc
     1021 acctgcagca agtcggcttc atcggacatt ggtgcccact ccttcacaga tcggccagta
     1081 cgcgcacatg ggcttccacg ctgcgcgcca gtggccccat cacgtagccg ccctccagac
     1141 aggagacgat gcgcttgttg gcaaagcgcc gcgccacgtc cttgatgcgc atggtgatcc
     1201 aggtgtagtc ctgctcggtc aggccgagct ggcccatgtc gtcgtcgcgg tgcgcgtcaa
     1261 agccggcgct gacaaagatc atctcgggct tgaaggcttc caggcgcggc atccagacca
     1321 tctccacgat ctcgcgcacg tccatgccct tggtgtaggc gggcacgggc acgttgagca
     1381 tgttgggcgc cggatcctgg tctccgctgt aggggtagaa ggggtgctgg aagatgctga
     1441 ccatgagggc gcgctcgtcg ccggccagga tgtcctcggt gccgttgcca tggtgcacgt
     1501 cgaaatcgat cacggccaca cgcttcaggt tgtggcgttg cagcgcatat ttggcagcca
     1561 cggccacgtt gttgaaaaag caaaagccca tggccttctt gtgctcggca tggtggccgg
     1621 gcgggcgcac gctgcagaag gcgttttcca gctcgccggc catgatggca tccacggcat
     1681 cgaccgccgc acccgctgcg cgcagcgccg ccgtccaggt gtggcgattc atggcggtgt
     1741 cggtgtcgag ctgaaaatag ggtgagccgc cggcctcttc ttcctcgacc aggcgcagcg
     1801 acaggcctcg cagcgccgcc acatgcatgc ggtcgtgtgc cagttcgatg tcggccaggg
     1861 aggccagcgg cgcctcgcgg tgatcgaggg cggccgacac gccggagacc aacagcctgt
     1921 cctcgatcgc atcaagccgc gccggacatt cggggtgacc cggccccatc tcgtgcaggc
     1981 ggcagtcgcg atgcgtgtaa tagcccgtct tgctcacagt caatctcccg tgtatcaatt
     2041 gttttcggat aacgtc
//
LOCUS       DAOMTQ010000287         2012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_17286_length_2012_cov_20.215125, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000287 DAOMTQ010000000
VERSION     DAOMTQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17286_length_2012_cov_20.215125"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..>2012
                     /locus_tag="PK518_15915"
     CDS             337..>2012
                     /locus_tag="PK518_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HRM50443.1"
                     /translation="MATLIPALGTCVARMTSGERRWAERLQDKLEDDYLLWWDVPIGP
                     KQTRPDFVVLHPRRGALILEVKDWRLDTIRRATREYFEIAPDGTPKVVMSPLQQARHC
                     AIQVVNALERDPQLVQGAGPYQGKLAFPWGHGVVFTHITRKQFEGAGLGEAIPAHLAI
                     CKDEMVEDVDAEAFQERLWAMFPHTFGSKGAITLPQLDRVRWILFPEVRVPQQQSLFG
                     SGAQGDDEDARLPDIMRVMDLQQEQLARSLGDGHRVIHGVAGSGKTMILGYRAEHLAQ
                     TQGPGSKPILILCYNEPLALKLAATMQAKGLDARAHVRHFHKWCRDQLVAYHLLPEGT
                     QRWPVDRLMQEYVQRVITGVDAGHIPAGQYHAILIDEGHDFAPEWLKLVTQMVDPTTN
                     SLLLLYDDAQSIYDRTKKKTFSLKSVGIQAAGRTTILKINYRNTRQILQLAHRMAGDM
                     LKPEDAEGGDDGIPLLQPLSCGREGPEPIVIDLPTLPERAARVAELLADQHAQGHAWG
                     DMAVLCRHHKEMDECSAALARKGLPHRVRKRSGDYRPEEDAIKVMTMHAS"
BASE COUNT          375 a          688 c          664 g          285 t
ORIGIN      
        1 ggcaaggacc gcgtcggcat gttcgagctg acgggcatcc tgggccagca cctgggctga
       61 tgcgcacgcc gccaggctga gccagcgcga gcgcatcggc tggggcggcc tggtggcctg
      121 gtggagcagg ccatcgcggg cgcagccgcg cagcgcatat ttgatagctg atagcgcttg
      181 ctgggtaagc gttaggggct gttttggctg tcaatccgcc gagcctgttt ctccgcactc
      241 gcctacggcg cacccggggt ttacgtcagt ttgaaatcag cttcgccgcc tataaggtgt
      301 ttcgagtcga aacaaacaag gggtaggcgg gggtggatgg cgacattgat accggccctg
      361 gggacatgcg tggcgcgcat gacatcgggc gagcgcagat gggccgagcg cctgcaagac
      421 aaactggaag acgattacct gctctggtgg gacgtgccca tcggccccaa gcagacgcgt
      481 cccgacttcg tggtgctgca cccgcgccgg ggcgcgctga tcctggaggt aaaggactgg
      541 cgcctggaca ccatccggcg cgccacgcgc gagtatttcg agatcgcgcc ggacggaacg
      601 cccaaggtcg tcatgagccc gctgcagcag gcgcggcact gcgccatcca ggtggtcaac
      661 gcgctggagc gcgacccgca actcgtgcag ggcgcggggc cgtaccaagg caagctggcc
      721 ttcccctggg gccacggcgt ggtcttcacc cacatcacgc gcaagcagtt cgagggcgcg
      781 ggcctgggcg aggccattcc cgcgcacctc gccatctgca aggacgagat ggtcgaggac
      841 gtggacgccg aagcgttcca ggagcggctg tgggccatgt tcccccacac cttcggcagc
      901 aagggcgcca tcacgctgcc gcagctcgac cgcgtgcgct ggatcctctt tcccgaagtg
      961 cgcgtgcccc agcagcaaag cctgtttggc tccggcgcgc aaggcgacga cgaggacgcc
     1021 cgcttgcccg acatcatgcg cgtgatggac ctgcagcaag agcaactggc ccgcagcctg
     1081 ggcgacggcc accgcgtgat ccacggcgtg gcgggctcgg gcaagaccat gatcctgggc
     1141 taccgcgccg agcacctggc gcaaacgcaa ggcccgggca gcaagcccat cctcatcctt
     1201 tgctacaacg agccgctggc cttgaagctg gccgccacca tgcaggccaa ggggctggat
     1261 gcgcgcgcgc acgtgcgcca cttccacaaa tggtgccgcg accagctcgt ggcctaccac
     1321 ctgctgcccg aaggcacgca gcgctggccc gtggacaggc tgatgcagga gtacgtgcag
     1381 cgtgtcatca ccggcgtgga tgcgggccac atccccgccg gccagtacca cgccatcctc
     1441 atcgacgagg gccacgactt cgcgcccgag tggctcaagc tggtcacgca gatggtggac
     1501 cccaccacca acagcctgct gctgctctac gacgacgcgc agagcatcta cgaccgcacc
     1561 aagaaaaaga ccttcagcct gaagagcgtg ggcatccagg ccgctggccg caccaccatc
     1621 ctgaagatca actaccgcaa cacgcggcaa atcctgcaac tggcccaccg catggccggc
     1681 gacatgctga agcccgagga cgccgagggc ggcgacgacg gcatccccct gctgcaaccc
     1741 ctgagctgcg gccgcgaagg ccccgagccc atcgtcatcg acctgcccac cctgcccgag
     1801 cgcgcggccc gggtggccga actgctggcc gaccagcacg cccagggcca cgcctggggc
     1861 gatatggccg tgctgtgccg acatcacaag gaaatggacg aatgctccgc cgccctggcc
     1921 cgcaaggggc tgccgcaccg ggtgcgcaaa cgttcaggcg attaccggcc ggaagaagac
     1981 gccatcaagg tgatgacgat gcatgcgagc aa
//
LOCUS       DAOMTQ010000288         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_17505_length_1996_cov_26.369912, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000288 DAOMTQ010000000
VERSION     DAOMTQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17505_length_1996_cov_26.369912"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK518_15920"
     CDS             <1..365
                     /locus_tag="PK518_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRM50444.1"
                     /translation="RRLVLAFQPHRYSRTRDCFEDFVKVLGGADAVLLTEVYAAGEAP
                     IVAADGRALARALRVAGSVEPVFIDDIADLPQRIASAARDGDVVLCMGAGSIGGVPAK
                     IVELLQKSELSAQEGRAQ"
     gene            362..1360
                     /locus_tag="PK518_15925"
     CDS             362..1360
                     /locus_tag="PK518_15925"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRM50445.1"
                     /translation="MSQKDLEINPPALGKVAVLMGGTSAEREVSLMSGGGVLQALRAR
                     GVDAHAFDPAHADLSELKTHGYNRCFIALHGRHGEDGTVQGALELLGIPYTGPGVMAS
                     AIAMDKIMTKRIWRFEGLPTPDWRLVASSAETAQAFAELGAPMIVKPSREGSTIGLTK
                     VTSLGQCEQAYLLAAQHDPEVLCEQFIDGDETTCPVLGQGDAARALPVIRIVAPDGNY
                     DYQNKYFTDVTQYHCPSGLSEAEEREIQRIVVQAYRTLGCRGWARADIMIRKGDRKPF
                     LLEINTSPGMTGHSLVPMSAKASGISYPDLCLRILASASLDALQGRAGAPGAQEHA"
     gene            1357..>1996
                     /locus_tag="PK518_15930"
     CDS             1357..>1996
                     /locus_tag="PK518_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRM50446.1"
                     /translation="MNATLPVPLDVKLMNMTATVMFLGCAAGVLAAGGAWLLRQPAFS
                     IAHIAVEGDVAHTSAMSLRANVAPQLKGNFFTVDLEAARRAFEQVPWVQSAQVRREFP
                     SGLRVHLQEHDVAAYWGAEGSATLVNSQGQVFEADADEVEEDGLPRLLAASPERAAEM
                     LDMARRLAPLLAPLGAGLETLELTGNSGWRVLLASGAVLQLGGGKPDEVLERV"
BASE COUNT          310 a          692 c          701 g          293 t
ORIGIN      
        1 gccggcgcct ggtgctggcc ttccagccgc accgctacag ccgcacgcgc gactgcttcg
       61 aggacttcgt caaggtgctg ggtggcgccg acgccgtgct gctgaccgag gtctatgccg
      121 ccggcgaggc gcccatcgtc gccgccgacg gccgcgccct ggcgcgcgcg ctgcgcgtgg
      181 ccggcagcgt ggagccggtg ttcatcgacg acatcgccga tctgccccag cgcatcgcca
      241 gcgccgccag ggatggcgac gtggtgctgt gcatgggcgc gggctcgatc ggcggcgtac
      301 ccgcgaagat cgttgaatta ctacaaaaaa gcgagctgtc agcgcaggaa ggacgggcgc
      361 aatgagccaa aaagacttgg agatcaaccc gcccgcgctg ggcaaggtgg cggtgctcat
      421 gggcggcacc tcggccgagc gcgaggtctc gctgatgtcc ggcggtggcg tgctgcaggc
      481 gctgcgcgcg cgcggcgtcg atgcccatgc cttcgatccg gcgcatgccg acctgtccga
      541 gctgaaaacc cacggctaca accgctgctt catcgccctg catggccgcc acggcgagga
      601 cggcacggtg cagggcgcgc tggagctctt gggcattccc tacaccggcc ccggcgtcat
      661 ggcctcggcc attgccatgg acaagatcat gaccaagcgc atctggcgct tcgagggtct
      721 gcccacgccc gactggcgcc tggtggccag cagtgccgag acggcacagg cctttgccga
      781 gctgggtgcg cccatgatcg tcaagccctc gcgcgagggc tcgaccattg gtctgaccaa
      841 ggtcacctcg ctcggccagt gcgagcaggc ctatctgctg gccgcccagc atgaccccga
      901 ggtgctgtgc gagcagttca tagacggcga cgaaaccacc tgcccggtgc tgggccaggg
      961 cgacgccgcg cgcgcgctgc cggtgatccg catcgtggcc cccgatggca actacgacta
     1021 ccagaacaag tacttcaccg acgtcacgca ataccactgc cccagcggat tgagcgaggc
     1081 cgaggagcgc gagatccagc gcatcgtggt gcaggcctac cgcactctgg gttgccgtgg
     1141 ctgggcgcgc gccgacatca tgatccgcaa gggcgaccgc aagcccttcc tgctggagat
     1201 caacacctcg cccggcatga ccggccattc gctggtgccc atgtcggcca aggccagcgg
     1261 catcagctat cccgacctgt gcctgcgcat tctggccagc gcctcgctgg atgcgctgca
     1321 gggccgtgct ggcgcgccgg gagcccagga gcacgcatga acgccacgct gcccgtaccg
     1381 ctggacgtca agctcatgaa catgaccgcc acggtcatgt tcctgggctg cgccgccggc
     1441 gtgctggcgg cgggcggggc ctggctgctg cgccagccag cgttttccat agcgcatatc
     1501 gccgtcgagg gcgatgtggc gcacaccagc gccatgagcc tgcgcgccaa cgtggcgccg
     1561 caactcaagg gcaacttctt caccgtcgat ctggaggctg cgcgccgcgc cttcgagcag
     1621 gtgccctggg tgcaaagcgc ccaggtgcgg cgcgaattcc ccagcggcct gcgcgtgcat
     1681 ctgcaggagc atgacgtggc cgcctactgg ggcgccgagg gcagcgccac gctggtcaac
     1741 agccagggcc aggtcttcga ggccgatgcc gacgaggtgg aagaggacgg cctgccgcgc
     1801 ctgctggcag cgtcgcccga gcgcgcggcc gaaatgctgg acatggcgcg gcgcctggcg
     1861 ccgctgctgg cgccgctggg agccggcctg gagacgctgg agctcaccgg caacagcggc
     1921 tggcgcgtgc tgctggccag cggcgccgtg ctgcagctgg gcggcggcaa gcccgatgag
     1981 gtgctggagc gcgtgc
//
LOCUS       DAOMTQ010000289         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_17602_length_1989_cov_28.869700, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000289 DAOMTQ010000000
VERSION     DAOMTQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17602_length_1989_cov_28.869700"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PK518_15935"
     CDS             complement(<1..545)
                     /locus_tag="PK518_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM50447.1"
                     /translation="MMNTLSRWYDSDVAHSFRSSPVAILAALIALVCAVCALFAGWVA
                     PHNPFDLSTLELSDARLPPWWLEGGTSKYPLGTDDQGRDILSALIYGARISLIVGLAS
                     VLLSVVLGVALGLVAGFRGGWIDGLLMRMCDVMLSFPAILIALLIAGVARAVFPNAPE
                     WLAFGVLIMAISLTGWVQYART"
     gene            complement(623..1843)
                     /locus_tag="PK518_15940"
     CDS             complement(623..1843)
                     /locus_tag="PK518_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRM50448.1"
                     /translation="MLAPRFKAGGRAFAQIAEFHPELTALRRDLHAHPELGFEEVYTS
                     ARVCEALRAAGVDEVHEGVGRTGVVGVIHGQGRSSGSMIGLRADMDALPMTEHNDFAW
                     KSAKSGLMHGCGHDGHTTMLVGAARYLAATRQFDGTAVLIFQPAEEGMGGARVMIEDG
                     LFDRFPVQSVYAMHNWPAMKPGTVGINSGAMMAAADRVTIDITGRGGHGAHPYQTVDV
                     VLVAGHIITALQSIVSRNVRALDSAVVSLCAVQAGDLGAFSVLPGQATLVGTVRAFDP
                     AVQDMVEQRIKDLCHATALGFGATATVRYERIYPATINTEAEARFAGDVAAALVGEDG
                     VDRDLEPSMGAEDFSFMLQSRPGAYLRLGQGMGAGNSTLHNSRYDFNDDILPLGAALH
                     AGLVERAMPLAGGQ"
     gene            complement(1843..>1989)
                     /locus_tag="PK518_15945"
     CDS             complement(1843..>1989)
                     /locus_tag="PK518_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM50449.1"
                     /translation="QAVTFADIPVMAAYLCLIALIFVVINLVVDLLYFVVDPRLRVGG
                     KGGH"
BASE COUNT          353 a          700 c          651 g          285 t
ORIGIN      
        1 gtgcgcgcgt actgcaccca gccggtcagc gaaatcgcca tgatcagcac gccgaaggcc
       61 agccactcgg gagcattcgg aaacaccgcg cgcgccacgc cggcgatcaa cagcgcgatc
      121 aggatggccg ggaaggacag catcacgtcg cacatgcgca tcagcaggcc atcgatccag
      181 ccgccgcgaa aacccgccac caggcccagg gccacaccca gcaccaccga cagcagcacc
      241 gaggccagac cgacgatgag cgagatgcgc gcgccgtaga tcagcgccga gaggatgtcg
      301 cgcccctggt catccgttcc cagcgggtac ttgctggtgc cgccctccag ccaccagggc
      361 ggcagacgcg cgtccgacag ctccagcgtg gacaggtcga aggggttgtg cggcgccacc
      421 cagccggcaa acagcgcgca gaccgcgcag accagagcaa tcagcgccgc cagaatggcc
      481 acgggcgagg atcggaaact gtgggccacg tcgctgtcat accagcgcga taaggtgttc
      541 atcatggctg caaatcgttg gttactatta aattagtagc ttgttgcgct tgatggacgg
      601 gcgctacagg ccatttttat gtttattgcc cgcccgccaa cggcatggca cgctccacca
      661 gcccggcatg cagcgccgcg cccaggggca ggatgtcgtc gttgaagtcg tagcggctgt
      721 tgtgcaaggt gctgttgccc gcgcccatgc cctggcccag gcgcaggtag gcgccggggc
      781 gggactgcag catgaaggag aaatcctccg cgcccatgct gggctccagg tcacggtcca
      841 caccatcctc gcccaccagg gcggcggcca cgtcgccggc aaagcgcgcc tcggcctcgg
      901 tgttgatggt ggcggggtag atgcgctcat agcgcaccgt ggccgtggcg ccaaagccca
      961 gggccgtggc gtggcacaag tccttgatgc gctgctccac catgtcctgc acggcggggt
     1021 cgaaggcgcg caccgtgccc accagcgtgg cctggcccgg cagcacgctg aaggcgccca
     1081 ggtcgccggc ctgcaccgcg cacaggctga ccaccgcgct gtccagcgcg cgcacattgc
     1141 gcgagacgat gctttgcagc gccgtgatga tgtgccccgc caccagcacc acatccaccg
     1201 tctggtaggg gtgggcgccg tggccgccgc gaccggtgat gtctatggtc acccggtcgg
     1261 cggcggccat catggcgccg ctgttgatgc ccacggtgcc gggcttcatc gccggccagt
     1321 tgtgcatggc gtacaccgac tgcacgggaa agcgatcgaa cagcccgtcc tcgatcatca
     1381 cgcgtgcccc gcccatgccc tcctcggccg gctggaagat cagcaccgcc gtgccgtcaa
     1441 actgccgcgt ggccgccaga tagcgcgccg cgcccaccag catggtggtg tggccgtcgt
     1501 ggccgcagcc gtgcatcagg ccgctcttgg cggacttcca ggcgaagtcg ttgtgctcgg
     1561 tcatcggcag tgcgtccatg tcggcgcgca ggccaatcat gctgccgctg ctgcgcccct
     1621 ggccgtggat cacgccgacc acgccggtgc ggcccacgcc ctcgtgcacc tcgtccacgc
     1681 cggcggcgcg cagcgcctca cacacgcggg cgctggtgta gacctcctca aagcccagct
     1741 cggggtgggc atgcaggtcg cggcgcagcg cggtgagctc ggggtgaaac tcggcaatct
     1801 gcgcaaaggc gcgcccgccg gccttgaagc gcggagccag catcaatgcc ctcccttgcc
     1861 acccacgcgc aggcgcggat cgacgacgaa gtacaacagg tccaccacca gattgatgac
     1921 cacgaagatc agcgcgatca ggcacaggta ggcggccatc accggaatgt cggcaaaggt
     1981 cacggcctg
//
LOCUS       DAOMTQ010000290         1985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_17652_length_1985_cov_23.101036, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000290 DAOMTQ010000000
VERSION     DAOMTQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17652_length_1985_cov_23.101036"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1869)
                     /locus_tag="PK518_15950"
     CDS             complement(133..1869)
                     /locus_tag="PK518_15950"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HRM50450.1"
                     /translation="MPSRTGTAHVVTTKRIYKDKVYCTHLLRRSYREDGKVKNETLGN
                     LSHLPEALIDIIRRSLQGETFVPVSQAFEVVRSRAHGHVQAVALAMQRLGLASVIASQ
                     PSRERDLVLAMVAARIVQPDTKLATCRRWHCSSLAEDFGVTDATEDDLYAAMDWLLAR
                     QDAIEHKLATRHLREDALVLYDLSSSYFEGSTCPLAKRGYSRDGRPGTLQVNYGLLTD
                     ARGCPVAVSVFEGNTSDSLTFLPAVQRVRERFGLQQVVMVGDRGMVSQKAIDELRGQG
                     GIDWITALKSVSIRGLVEQGHLQLGLFDQRNLAEITSPEYPGERLVACRNDALAQLRA
                     HKRESLLQATEALLAQVLASVQAGRLTGQDKIGVQVGRVINRHKVAKHFELSIAETTL
                     GWARRQDAIEAEAALDGLYVIRTSLEAQRMDAPSCVRSYKALSNVERAFRSLKTMDLK
                     VRPIHHRLAERVRAHILLCMLAYYVEWHMRQAWAPLMFADTDQAAKATRDPVAPAKRS
                     AAAQRKAQSKVIDDGQPAHSFATLMAEMATIVRNTCRTPSADPDAPTFELLTTATAHQ
                     QRALALIQAIRP"
BASE COUNT          328 a          627 c          672 g          358 t
ORIGIN      
        1 gccgcgccct gggtgaggcg ttggccacgg gtttggcctt gggcgtgttt taggctgaag
       61 ttcccccgcc gtcagcgcga attttcctgt tccatcaacg acttgcgatg gattgcgggt
      121 ttgggtttct gactacggac ggatggcctg gatcagcgcc agggcgcgct gctggtgggc
      181 ggtggcggtg gtgagcagtt cgaaggtcgg tgcatccgga tcggcgctgg gcgtgcgaca
      241 ggtgttgcgc acgatggtgg ccatctcggc catcagggtg gcgaagctgt gcgcgggctg
      301 accatcatcg atcaccttgg attgggcctt gcgctgcgca gcagccgagc gcttggccgg
      361 cgccacaggg tcgcgcgtgg ccttcgccgc ctgatcggtg tcggcgaaca tcagcggcgc
      421 ccaagcctgg cgcatgtgcc actcgacgta gtacgccagc atgcacagca ggatgtgcgc
      481 gcgcacccgc tcagccaggc gatggtggat ggggcgtacc ttcagatcca tcgtcttcaa
      541 tgaacggaac gcccgctcca cattggacag cgccttgtag ctgcgcacgc agctcggtgc
      601 atccatgcgc tgtgcctcca gcgaggtgcg aatgacgtac aacccatcga gcgccgcctc
      661 cgcctcgatg gcgtcctgcc ttcgcgccca gcccaacgtc gtctcagcga tgctcagctc
      721 aaagtgcttg gccaccttgt ggcggttgat gacccggccc acctgcacgc cgatcttgtc
      781 ctgccccgtg agccggcccg cctggacgct ggccaagacc tgcgccagca gggcctcggt
      841 ggcctgcagc agactctcgc gcttgtgcgc acgcagctgg gccagggcgt cgttgcgaca
      901 ggccaccagg cgttcgccgg gatactcggg tgaagtgatc tcggccaggt tgcgctgatc
      961 gaacaagccc agctgcaagt ggccttgctc gaccaggcca cggatcgaga cgctcttgag
     1021 cgcggtgatc cagtcgatgc cgccttggcc gcgaagctcg tcgatggcct tttgcgacac
     1081 catgccgcgg tcgcccacca tcaccacctg ctgcaggccg aagcgctcgc gtacgcgctg
     1141 cacggctggc aggaaggtca ggctgtccga ggtattgccc tcgaacaccg agacggccac
     1201 cgggcagccg cgcgcatcgg tgagcagccc atagttgacc tgcagcgtgc cggggcggcc
     1261 gtcacggctg tagccgcgct tggccagcgg gcaggtactg ccctcaaagt agctcgacga
     1321 caggtcgtac agcaccaacg cgtcctcgcg caggtggcgc gtggccagct tgtgctcgat
     1381 ggcgtcctgg cgcgccagta gccaatccat ggcggcatac aggtcgtcct cggtggcgtc
     1441 cgtcacgcca aagtcctcgg ccaggctcga gcagtgccag cgcctgcagg tggccagctt
     1501 ggtgtcgggc tggacgatgc gcgcggccac catggccagc accaggtcac gttcacggct
     1561 gggctgagag gcgatcaccg aggccagtcc cagacgctgc atggccaggg ccacggcctg
     1621 cacgtgacca tgggcacgcg agcgcacgac ctcgaaggcc tgggagacgg ggacgaaggt
     1681 ctcgccctgc agcgagcgcc ggatgatgtc gatcagcgct tcgggcagat gcgacaggtt
     1741 gcccagggtc tcgttcttga ccttgccgtc ttcgcggtag ctgcggcgca gcaggtgggt
     1801 gcaatagacc ttgtccttgt agatccgctt ggtggtgacg acgtgagcag ttccagttcg
     1861 tgacggcata gggcagataa tgcggcgatt tgagtgcaat gtcaattgtt ttagtgacta
     1921 cacgtccgga cgaaaaaaag gcccgaaagg gccaaattcg tgaagcacga caaggactta
     1981 ccggc
//
LOCUS       DAOMTQ010000291         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_17823_length_1971_cov_29.379436, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000291 DAOMTQ010000000
VERSION     DAOMTQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17823_length_1971_cov_29.379436"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /gene="aceE"
                     /locus_tag="PK518_15955"
     CDS             <1..1937
                     /gene="aceE"
                     /locus_tag="PK518_15955"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRM50451.1"
                     /translation="RENLDNLIFVINCNLQRLDGPVRGNGKIVQELEGDFRGAGWHVI
                     KLLWGKGWDELLARDKSGKLKQLMMETLDGDYQTYRAMDGAYIRKHFFGKYPETLKLV
                     EHLTDDEIWELRRGGHEPEKVYAAFHAANEHHGEPTVLLVKTVKGYGMGKAGEAKNTV
                     HQTKKLDDEDIKYIRDRFNIPIPDSELEKLPYYKPADDTPEMQYLHERRKALGGYLPK
                     RREQADEHFTVPALDTFKAVIEPTAEGREISTTQAYVRFLTQLLRDKALGPRVVPILV
                     DEARTFGMEGLFRQIGIYNPLGQLYTPVDRDQVMYYKEDKAGQILQEGINEAGGICSW
                     IAAATSYSTNNRIMIPFFVYYSMFGFQRFGDFAWAAGDMQARGFILGGTSGRTTLNGE
                     GLQHEDGHSHILAGTIPNCISYDPTFAHEVGVILHAGLKRMVEKQENVYYYITLLNEN
                     YAMPGLTAGTEEQILKGMYLCKQGAEGALRVQLLGSGSILRESIEAQKILAADWGVQS
                     DVWSCTSFNELTREGQDAERHNLLHPTATPRVPFVTQQLTGHAGPVVASTDYMKNYAE
                     QIRAFIPAGRSYKVLGTDGFGRSDFRSKLREHFEVNRHFITVAALKALADDGKLPLAK
                     VAEAIARYGIKTDKINPLHA"
BASE COUNT          412 a          649 c          609 g          301 t
ORIGIN      
        1 cgcgcgagaa cctggacaac ctgatcttcg tcatcaactg caacctgcag cgcctcgatg
       61 gcccggtgcg cggcaatgga aagatcgtgc aggagctcga aggcgacttc cgcggcgccg
      121 gctggcatgt catcaagctc ttgtggggca agggctggga cgagctgctg gcgcgcgaca
      181 agagcggcaa gctcaagcaa ctgatgatgg agacgctgga cggcgactac cagacctacc
      241 gcgccatgga cggcgcctac atccgcaagc atttcttcgg caagtacccc gagacgctca
      301 agctggtcga acacctgacc gatgacgaga tctgggagct gcgccgcggt ggccacgagc
      361 ccgagaaggt ctacgccgcc ttccatgcgg ccaatgagca ccatggcgaa cccacggtgc
      421 tgctggtgaa gaccgtcaag ggttacggca tgggcaaggc cggtgaggcc aagaacaccg
      481 tgcaccagac caagaagctc gacgacgagg acatcaagta catccgcgac cgcttcaaca
      541 ttccgatccc ggacagcgag ctggaaaagc tgccctacta caagcctgcc gacgatacgc
      601 ccgagatgca gtacctgcac gagcgccgca aggccctggg tggctacctg cccaaacgcc
      661 gcgagcaggc cgacgagcat ttcaccgtgc ccgcgctgga taccttcaag gccgtcattg
      721 agcccacggc cgagggccgc gagatcagca ccacccaggc ctatgtgcgc ttcttgacgc
      781 aactgctgcg cgacaaggca ctgggcccgc gcgtcgtgcc catcctggtc gatgaggcgc
      841 gcaccttcgg catggagggg ctgttccgcc agatcggcat ctacaacccg ctgggccagc
      901 tctacacccc cgtcgaccgc gaccaggtga tgtattacaa ggaagacaag gccgggcaga
      961 tcctgcaaga gggcatcaac gaggcgggcg gcatctgctc gtggattgcg gcggctacgt
     1021 cgtactcgac gaacaaccgc atcatgatcc cgttcttcgt ctactactcg atgttcggct
     1081 tccagcgctt tggcgacttt gcctgggccg ccggcgacat gcaggcgcgc ggcttcatcc
     1141 tgggcggcac gtccgggcgc accacgctca atggcgaggg cctgcagcac gaggacggcc
     1201 acagccacat cctggcgggc accattccca actgcatcag ctacgacccg acgtttgcgc
     1261 atgaggtcgg cgtgatcctg cacgcaggcc tgaagcgcat ggtggaaaag caggagaacg
     1321 tgtactacta catcaccctg ctgaacgaaa actacgccat gccgggcctg acggccggca
     1381 ccgaggagca gatcctgaag ggcatgtatt tgtgcaagca gggcgccgaa ggcgcgttgc
     1441 gcgtgcagct gctgggttcg ggctccatcc tgcgcgaatc catcgaggcg cagaagatcc
     1501 tggccgccga ctggggcgtg cagtccgacg tctggagctg caccagcttc aatgagctca
     1561 cccgtgaggg ccaggacgcc gagcgccaca acctgctgca ccccacggcc acgccgcgcg
     1621 tgcccttcgt cacgcaacag ctcaccggtc acgccggccc ggtggtggct tcgaccgact
     1681 acatgaagaa ctacgccgag cagatccgcg ccttcatccc ggccgggcgc agctacaagg
     1741 tgctgggcac cgacggcttt ggccgcagcg acttccgcag caaactgcgt gagcatttcg
     1801 aggtcaaccg ccacttcatc accgtggccg ccctgaaggc gctggccgac gatggcaagc
     1861 tgccgctggc caaggtggcc gaggcgattg ccaggtacgg catcaagacc gacaagatca
     1921 accccctgca cgcctgaaga cgagggagac aaccatggca ttgattcaaa t
//
LOCUS       DAOMTQ010000292         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_17824_length_1971_cov_26.059499, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000292 DAOMTQ010000000
VERSION     DAOMTQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17824_length_1971_cov_26.059499"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1613)
                     /locus_tag="PK518_15960"
     CDS             complement(33..1613)
                     /locus_tag="PK518_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM50452.1"
                     /translation="MQFRALFLSMAAASLLAAVPAAHAKTFKWASQGEISTWDIHSQN
                     NALQNGLHSYVYETLVTYNSRTFAVEPLLATAWREVSPTQVRFTLRQGVKFQDGSAFT
                     ADDAVYSLQRAMSKTSNYTPYVQGITKVVKVDANTMDVFLAAPNPVLLRQMTELRMMS
                     KAWAEKNKSVEPKDMKGKDENFAHRNAMGTGPYTLESWQPDVKMVFKRNPNWWGQMDG
                     NVTEIVYTPIKSAATRIAALLSGEVDMVLDPSPQDLQRLRSTDDIRVVDGLENRTIFL
                     GMDQFRDELVGSNVKGKNPLKDLRVRKALYQAIDANALTRNILRGLGKPTGTLVAPQV
                     AGWTEAVGNRLPYDVEGAKKLLAEAGYAEGFEVDFACPNNRYINDEAICQAVTAMWAR
                     IGVKAKLRTLPLVSYFPMIQRSEASIYMLGWGVPTFDALYSLQSLTRSVGQGGDGNYN
                     VGRYKNERMDYLVDRVKVETDAPVRTRMLTEALQLSGETVSHIPLYDQVIPWAMKKKV
                     ELVHRADNRVDMRLVKIN"
     gene            complement(1772..>1971)
                     /locus_tag="PK518_15965"
     CDS             complement(1772..>1971)
                     /locus_tag="PK518_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRM50453.1"
                     /translation="GSATGAFNGNANFYVAVADYAFSKRTDAYFGVDHTSISGGGAVV
                     LDTASKARSRTGITIGLRHRF"
BASE COUNT          347 a          629 c          617 g          378 t
ORIGIN      
        1 ccctctcccg ccagcgggag agggtgcaaa aatcagttga tcttcaccag ccgcatatcc
       61 acccggttgt ccgcgcggtg caccagctcc acctttttct tcatggccca ggggatgacc
      121 tggtcgtaca gcggaatatg cgacacggtt tcgcccgaga gctgcagcgc ctcggtcagc
      181 atgcgcgtgc gcacgggggc gtcggtctcc accttgacgc ggtctaccag gtagtccatg
      241 cgctcatttt tgtagcgccc cacgttgtaa ttgccgtcgc ccccctggcc cacgctgcgc
      301 gtgagcgact gcaggctgta cagcgcatca aaggtcggca cgccccagcc cagcatgtag
      361 atgctggcct cgctgcgctg gatcatgggg aagtagctca ccagcggcag ggtgcgcagc
      421 ttggccttga cgccaatgcg cgcccacatc gcggtcaccg cctggcaaat ggcctcgtcg
      481 ttgatgtagc ggttgttcgg acaggcgaag tccacctcga aaccctcggc gtagccggcc
      541 tcggccagca gcttcttggc gccttccacg tcatagggca ggcgattgcc cacggcctcg
      601 gtccagcccg ccacctgcgg cgccaccagg gtgcccgtgg gtttgcccaa gccgcgcagg
      661 atgttgcgcg tgagcgcgtt ggcgtcgatg gcctggtaca aggccttgcg cacgcgcaag
      721 tccttgagcg ggttcttgcc cttcacattg gagcccacca gctcgtcgcg aaactgatcc
      781 atgcccagga agatggtgcg gttctccagg ccatcgacca cgcggatgtc gtccgtggaa
      841 cgcaggcgct gcagatcctg cggcgaaggg tcgagcacca tgtccacctc gcccgagagc
      901 agcgcggcga tgcgcgtagc cgccgacttg atcggcgtgt agacgatctc ggtcacattg
      961 ccgtccatct ggccccacca gttcgggttg cgcttgaaga ccatcttcac gtccggctgc
     1021 caagactcca gggtgtacgg ccccgtgccc atggcattgc ggtgggcgaa gttctcgtcc
     1081 ttgcccttca tatccttggg ttcgaccgac ttgttcttct cggcccaggc cttgctcatc
     1141 atgcgcagct cggtcatctg gcgcagcagc accgggttgg gcgcggccag gaacacatcc
     1201 atggtgttcg cgtccacctt caccaccttg gtgatgccct gcacataggg cgtgtagttg
     1261 gaggtcttgg acatggcgcg ctgcagcgaa tacacagcgt cgtcggccgt gaaagccgaa
     1321 ccgtcctgga acttcacgcc ctggcgcagc gtgaagcgca cctgcgtcgg gctgacctcg
     1381 cgccaggcgg tggccagcag cggctcgacc gcgaacgttc ggctgttgta ggtcaccagg
     1441 gtttcgtaca cataggaatg caggccgttt tgcagcgcgt tgttctgcga gtggatgtcc
     1501 caggtcgata tctcgccctg gctggcccat ttgaaggtct tggcatgggc ggccggtacg
     1561 gccgccagca aggatgcggc ggccatggac aggaacaagg cacggaattg catgggaagc
     1621 ctcctgaaaa acaaaacccg caactatggc agcgcactag caagatgcat gcgaggggaa
     1681 acccccaggg tttgatttgc ttgcgtcttg cgcggcatca aagcaagaaa cctcccgcag
     1741 gaggtttctt gcaagccggt tcgaaaccgc gtcagaagcg atggcgcaag ccgatggtga
     1801 tgccggtgcg gctgcgggcc ttgctggccg tgtccagcac gaccgcaccg ccaccgctga
     1861 tgctggtgtg atccacacca aagtaggcat cagtacgctt gctgaaggcg tagtcggcga
     1921 ccgccacata gaagttggca ttgccattga aagccccggt ggccgagccc g
//
LOCUS       DAOMTQ010000293         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_18024_length_1958_cov_23.459800, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000293 DAOMTQ010000000
VERSION     DAOMTQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18024_length_1958_cov_23.459800"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="PK518_15970"
     CDS             <1..1602
                     /locus_tag="PK518_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM50454.1"
                     /translation="AWARRAAHLGAAVQRGYRRSLAWGLRHQPLVLLALAGVVALNVH
                     LYRSIDKGFMPDQDTGRIMGFIRADQATSYQAMEQRLKRFLAIVQEDPAVEYVTGFTG
                     GGQRNAASMFMTLKPMAQRQASSDQVIARLRDKLRNEPGARLFMMTQKDIRIGGRQSS
                     ASYDYTLQADDIADLRTWEPRVRQALARLPELEDVNSDVQDFGQQTLLEIDRDAATRL
                     GLTVAQIDATLNDAFGQRQVGVIFNPLNQYRVVMEAAPRYLQSPETLAGFFFVNKDGR
                     QVPLTAFARIARGTTPLSVNHQGGTPASTIGYSLAPGVSLSQANDAIRDAVAGLGVPV
                     SVRGSFSGTAGAFQQALAGQPLLILAAIVTIYLVLGMLYEDLVHPITILSTLPSAGVG
                     ALLALMLFKTEFSLIALIGVLLLIGIVKKNAILMIDFALARQKSGRGTPVQAIYRACH
                     LRLRPILMTTVAAIFGALPLALGHGVGAELRQPLGIAIVGGLLVSQLLTLYTTPVVYV
                     LLERLRRRLRARRGGGAAVLNQTGL"
     gene            1621..>1958
                     /locus_tag="PK518_15975"
     CDS             1621..>1958
                     /locus_tag="PK518_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HRM50455.1"
                     /translation="MQLSKVLIISLPIALTLLAGCASAPPYQRPAIALPAQFKQATAA
                     AQDAGIWRPVQPQDAPAPEAWWRLYADPHLDRLQQQAASANPGIAQAVARLRAAQAAV
                     AGSRAGLLPSL"
BASE COUNT          300 a          701 c          658 g          299 t
ORIGIN      
        1 gcctgggcac gccgggccgc gcacctgggc gcggccgtgc agcgcggcta ccgccgcagc
       61 ctggcctggg gcctgcgcca ccagccgctg gtgctgctgg cgctggcggg cgtggtggcg
      121 ctgaacgtgc acctgtaccg gtccatagac aagggcttca tgccggacca ggacacgggc
      181 cgcatcatgg gtttcatacg cgccgaccag gccacgtcct accaggccat ggaacagcgc
      241 ctgaagcgtt ttctggccat cgtgcaggaa gacccggcgg tggaatatgt caccggcttt
      301 accggcggcg gccagcgcaa cgcggccagc atgttcatga cgctcaagcc catggcgcag
      361 cgccaggcct cgtcggatca ggtcatcgcc cgcctgcgcg acaagctgcg caacgagccc
      421 ggcgcgcgcc tgttcatgat gacgcaaaag gacatacgca taggcgggcg gcaaagcagc
      481 gcgtcgtacg actacacgct gcaggccgac gacatcgccg acctgcgcac ctgggagccg
      541 cgcgtgcgcc aggcgctggc gcgcctgccg gagctggagg acgtgaacag cgacgtgcag
      601 gacttcggcc agcagaccct gctggagata gaccgcgacg ccgccacgcg cctgggcctg
      661 acggtggcgc agatcgacgc caccttgaac gacgccttcg gccagcgcca ggtgggcgtg
      721 atcttcaacc ccttgaatca gtaccgtgtg gtcatggagg ccgcgccgcg ctacctgcaa
      781 agccccgaga ccctcgccgg cttcttcttc gtcaacaagg acggccggca ggtgccgctg
      841 acggcctttg cgcgcatcgc gcgcggcacc acgccgctgt cggtcaacca ccagggcggc
      901 acgccggcca gcaccatcgg ttacagcctg gccccgggtg tgtcgctgtc gcaggccaac
      961 gacgccatac gcgacgccgt ggccggcctg ggcgtgccgg tgtcggtgcg cggcagtttc
     1021 agcggcacgg ccggggcctt tcagcaggcg ctggcgggcc agccgctgct gatactggcg
     1081 gccatcgtca ccatctacct ggtgctgggc atgctgtatg aagacctggt gcaccccatc
     1141 accatcctct ccacgctgcc ctcggccggc gtcggggcgc tgctggcgct gatgctgttc
     1201 aagaccgagt tctcgctgat cgcgctgatc ggcgtgctgt tgctcatcgg catcgtgaag
     1261 aagaacgcca tcctgatgat cgactttgcg ctggcgcggc agaaaagcgg ccgcggcacc
     1321 cccgtgcagg ccatctaccg cgcctgccac ctgcgcctgc gccccatcct gatgaccacc
     1381 gtggctgcca tcttcggcgc gctgccgctg gccctgggcc acggcgtggg cgccgagctg
     1441 cgccagccgc tgggcattgc catcgtcggc ggcctgctgg tgagccagct gctcacgctc
     1501 tacaccacgc ccgtggtcta tgtgctgctg gagcgcctgc gccggcgcct gcgggcgcgg
     1561 cgcgggggtg gtgcagcggt tttgaatcaa acagggctct aacgcttaca ggacaagcgc
     1621 atgcagctat caaaagtatt aattattagc cttcctatcg ccctgaccct attggctggc
     1681 tgcgccagcg cgccgcccta ccagcgcccg gcgatagcgc tgcccgcgca gttcaagcag
     1741 gccacggcag ccgcgcagga tgccggcatc tggcgcccgg tgcagccaca ggacgcaccg
     1801 gcacccgagg cgtggtggcg tctgtatgcc gacccgcatc tggaccgcct gcagcagcag
     1861 gccgcgagcg cgaatcccgg catcgcccag gccgtggcac ggctgcgcgc cgcccaggcg
     1921 gccgtggcgg gcagccgcgc cggcctgctg cccagcct
//
LOCUS       DAOMTQ010000294         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_18245_length_1941_cov_28.060445, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000294 DAOMTQ010000000
VERSION     DAOMTQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18245_length_1941_cov_28.060445"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1486)
                     /locus_tag="PK518_15980"
     CDS             complement(68..1486)
                     /locus_tag="PK518_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRM50456.1"
                     /translation="MEQLQEFFTRETPRMAALADQIWSLAELRYAETASARLHMDALA
                     ASGFRVTPDVAGIPTAFMAEWGDSGPVIAFLGEYDALSGLNQQSGALVCTPSVDSPGL
                     AGHGCGHHLLGTSAHFAAVALQAHLLASGQKARVRYYGCPAEEGGSGKTFMARAGVFD
                     DVDVALTWHPGSFTSVFSQTTLANIQAKFIFHGKASHAAHSPHLGRSALDAVELMNVG
                     VNYLREHMPSDARVHYAVTDSGGLSPNVVQARAEVLYLIRAAQNHEAAELYERVQNVA
                     RGAALMTDCKVDIVFDKACSNLLQNRTLNQVLYAKLLAEGALTVDAGEQGQAGRFQAT
                     LSKNDLDAVSRPLASVLRGQVPAVFEGVAPYDPAVNEILFGSTDVADVSWITPTVQAW
                     VACYAFGTPMHSWQMVSQGQSALAHAGMVRAARVLTATAIELVNRPELIAQAQAELLE
                     RRQGKPYECPIPADVPLPFLRT"
     gene            complement(1498..>1941)
                     /locus_tag="PK518_15985"
     CDS             complement(1498..>1941)
                     /locus_tag="PK518_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRM50457.1"
                     /translation="IQIVPYRGAGPALNDALAGQVQLMPDQLPSAMPHIKSGKLIPFV
                     QASDGRSPDLPNVPTFKELGYEGLNQLGISWFGIVVPKNTPAPIVKRLQEAAVKATHL
                     PEVQQRLKTVGANASDMDQGQFPAQIARESQRNKALLEKIGVKPE"
BASE COUNT          286 a          655 c          682 g          318 t
ORIGIN      
        1 tggccaaggc cacatattga tcaaaatggc ctgtagcgcc cgccaatagg gcgcagcagc
       61 tatcaaatca ggtgcgcaag aatggcaggg gtacatccgc cggtatcggg cactcatagg
      121 gcttgccctg gcggcgctcc agcagctccg cctgggcctg ggcgatgagt tcgggccggt
      181 tcaccagttc gatggccgtg gccgtgagca cgcgcgcggc gcgcaccatg ccggcatggg
      241 ccagggcgga ttggccctgc gacaccatct gccaggagtg catgggcgtg ccgaaggcgt
      301 agcaggccac ccaggcctgg actgtgggcg tgatccagct cacgtcggcc acgtcggtgg
      361 agccgaacag gatctcgttg accgccgggt cgtagggtgc cacgccctcg aacacggcgg
      421 gcacctgacc gcgcagcacg ctggccagcg gccggcttac cgcatccagg tcgttcttgc
      481 tcagcgtggc ctggaagcgc ccggcctggc cctgctcgcc ggcgtccacg gtgagcgcgc
      541 cctcggccag cagcttggcg tacagcacct ggttcagcgt gcggttctgc agcaggttgg
      601 agcaggcctt gtcgaagacg atgtccacct tgcagtcggt catcagcgcc gcgccgcggg
      661 ccacgttctg cacgcgctcg tacagctcgg cggcctcgtg gttctgcgcc gcgcgtatca
      721 ggtacagcac ctcggcgcgc gcctgcacca cgttgggcga caggccgccc gagtcggtca
      781 cggcgtagtg cacgcgcgcg tcggacggca tgtgctcgcg caggtagttc acgcccacgt
      841 tcatcagctc caccgcgtcc agcgcgctgc gccccaggtg cggcgaatgc gcggcgtgcg
      901 aggccttgcc gtggaagatg aacttggcct ggatgttggc cagcgtggtc tggctgaaca
      961 cgctggtgaa gctgcccggg tgccaggtca gggccacatc cacgtcgtcg aacacgccgg
     1021 cgcgcgccat gaaggtcttg cccgagccgc cctcctcggc cgggcagccg tagtagcgca
     1081 cgcgcgcctt ttggccgctg gccagcagat gcgcctgcag cgcgaccgcc gcaaagtgcg
     1141 ccgaggtgcc cagcaggtgg tggccgcagc cgtggcccgc caggcccggg ctgtccacgg
     1201 atggcgtgca caccagggcg ccgctttgct ggttcagccc cgacagggcg tcgtactcgc
     1261 ccagaaaggc gatcaccggg cccgagtcgc cccattcggc catgaaggcc gtgggaatgc
     1321 cggccacatc cggcgtgacg cgaaagcccg aggcggccag cgcgtccatg tgcagccggg
     1381 ccgatgccgt ctcggcatag cgcagctcgg ccagcgacca gatctggtcg gccagcgcgg
     1441 ccatgcgcgg cgtctcgcgc gtgaagaatt cctgcagttg ttccatgctt gcctcgctca
     1501 ctcgggcttc acgccgatct tctcgagcag cgccttgttg cgctgcgact cgcgcgcgat
     1561 ctgggccggg aactggccct gatccatgtc gctggcattg gcgcccacgg tcttcaggcg
     1621 ctgttgcacc tcgggcagat gcgtggcctt gacggccgcc tcctgcaggc gcttgacgat
     1681 gggcgcgggc gtgttcttgg gcacgacgat gccaaaccag ctgatgccca gctggttcag
     1741 gccctcatag cccagctcct tgaaggtggg cacgttgggc aggtcgggcg agcggccgtc
     1801 ggaggcctgg acaaaaggaa tcagcttgcc gctcttgatg tgcggcatgg cggacggcag
     1861 ctggtcgggc atgagctgca cctggccggc cagcgcgtcg tttagcgccg ggccggcgcc
     1921 gcggtagggc acgatctgga t
//
LOCUS       DAOMTQ010000295         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_19224_length_1876_cov_29.049423, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000295 DAOMTQ010000000
VERSION     DAOMTQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19224_length_1876_cov_29.049423"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="murG"
                     /locus_tag="PK518_15990"
     CDS             <1..1065
                     /gene="murG"
                     /locus_tag="PK518_15990"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRM50458.1"
                     /translation="KTALIMAGGTGGHIFPGLAVAEELKARGWRVHWLGTPGSMESRI
                     VPQHGLPLETIDFSGVRGKGVITLALLPLNLLRAFWQALAVVRRVRPDVVIGMGGYVT
                     FPGGVMAVAAGKPLLLHEQNSVAGMANRVLASMADRIFTAFPGVFKKGHWVGNPLRAA
                     FTRQAGPAERFAGRTGPLKLLVVGGSLGARALNDIVPQALALLPEAQRPQVIHQSGQG
                     VNGGQIEQLRANYQAAGVQAELTPFIDDTAAAFAEADVIVCRAGASTVTEIAAVGAAA
                     VFVPFPHAVDDHQSTNARFLVDAGGGWLVQQADLTPRGLADMLLNMERSALLERALKA
                     KNMQKTQATREVVSACEELA"
     gene            1062..>1876
                     /locus_tag="PK518_15995"
     CDS             1062..>1876
                     /locus_tag="PK518_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRM50459.1"
                     /translation="MKHAIHHIHFVGIGGSGMCGIAEVLHNLGYKVSGSDLADSATLA
                     RLRTLGVATHVGHAAAHIAGADAVVTSTAVQQDNPEVLAARERKIPVVPRAVMLAELM
                     RLRKGIAIAGAHGKTTTTSLVASVLAEGGLDPTFVIGGRLNSAGTNAKLGGGEYIVVE
                     ADESDASFLNLLPVMAVVTNIDADHMETYGHDFGRLKQAFVDFLHRMPFYGTAILCVD
                     NPAIRDILPRVTCPVTSYGLAEDAEVRAVDVRAVGTQMHFTVLRRNGVTLPDL"
BASE COUNT          288 a          642 c          654 g          292 t
ORIGIN      
        1 aagaccgccc tcatcatggc cggcggcacc ggcggccata tcttccccgg cctggccgtg
       61 gccgaggagc tcaaggcacg gggctggcgc gtgcactggc tgggtacgcc cggcagcatg
      121 gaatcgcgca tcgtgccgca gcatggcctg ccgctggaga ccatcgactt ttccggcgtg
      181 cgcggcaagg gcgtgatcac gctggcactg ctgccgctga atttgctgcg cgccttctgg
      241 caggcgctgg ccgtggtgcg gcgcgtgcgg cccgatgtcg tcatcggcat gggcggctat
      301 gtcacctttc ccggcggcgt gatggccgtg gcggcgggca aaccgctgct gctgcacgag
      361 cagaactcgg tggccggcat ggccaaccgg gtgctggcca gcatggcgga tcgcatcttc
      421 acggccttcc ccggcgtgtt caaaaaaggc cactgggtgg gcaacccgct gcgcgcggcc
      481 ttcacgcggc aggccggccc ggccgagcgc tttgccggcc gcaccgggcc actcaaactg
      541 ctggtggtgg gcggcagcct gggcgcgcgc gcgctcaacg acatcgtgcc ccaggcgctg
      601 gcgctgctgc ccgaggcgca gcgcccccag gtcatacacc agagcggcca aggtgtgaat
      661 ggagggcaga tcgagcagct gcgcgccaat taccaggccg ccggcgtgca ggccgagctg
      721 acgcccttca tcgacgacac ggccgcggcg ttcgccgagg ccgacgtcat cgtctgccgc
      781 gccggcgcca gcaccgtcac cgagatcgcg gccgtgggcg cggccgcggt gttcgtgccg
      841 tttccgcatg cggtggacga ccaccagagc acgaacgccc gctttctcgt cgatgcgggc
      901 ggcggctggc tggtgcagca ggccgacctg acgccgcgcg gcttggccga tatgctatta
      961 aatatggagc gttccgcgct tttggagcgg gcgctgaagg caaaaaacat gcaaaaaacc
     1021 caggccaccc gcgaggtggt gtccgcctgc gaggagttgg catgaaacac gccattcatc
     1081 acatccactt cgtcggcatc ggcggctcgg gcatgtgcgg cattgccgag gtgctgcaca
     1141 acctgggcta caaggtgtcg ggctcggatc tggccgacag cgccacgctg gcgcgcctgc
     1201 gcacgctggg cgtggccacc catgtgggcc acgcggccgc gcatatcgcg ggcgcggacg
     1261 ccgtggttac ctccacggcg gtgcagcagg acaaccccga ggtgctggcc gcgcgcgaac
     1321 gcaagattcc cgtcgtgccg cgtgccgtga tgctggccga gctgatgcgc ctgcgcaagg
     1381 gcatcgccat tgccggcgcg catggcaaga ccaccaccac cagcctggtg gccagcgtgc
     1441 tggccgaggg cgggctcgat cccaccttcg tcatcggcgg gcgcctgaac agcgccggca
     1501 cgaatgccaa gctgggcggg ggcgagtaca tcgtggtcga ggcggatgaa tccgacgcct
     1561 cgttcctgaa cctgctgccg gtgatggcgg tcgtcaccaa catcgatgcc gaccatatgg
     1621 agacctatgg ccatgacttt ggccggctca agcaggcctt cgtggacttt cttcaccgca
     1681 tgccgttcta cggcacggcc atcctgtgcg tggacaaccc ggccatacgc gacatcctgc
     1741 cgcgcgtgac ctgcccggtg accagctacg gcctggccga ggatgccgag gtgcgcgccg
     1801 tcgacgtgcg cgccgtgggc acgcagatgc acttcaccgt gctgcggcgc aacggcgtca
     1861 cgctgcccga cctgca
//
LOCUS       DAOMTQ010000296         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_19290_length_1872_cov_25.412768, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000296 DAOMTQ010000000
VERSION     DAOMTQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19290_length_1872_cov_25.412768"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..946
                     /gene="slmA"
                     /locus_tag="PK518_16000"
     CDS             308..946
                     /gene="slmA"
                     /locus_tag="PK518_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HRM50460.1"
                     /translation="MSEFTPTYPESSKPDEARVTRRRLQPGERREQILQALAAMLQQP
                     GAERVTTAALARQLGVSEAALYRHFASKAQMFEGLIDFIEHSVFTLVRQIEEQGDQAN
                     GALRAARIAAMVLQFGEHNPGMVRVMVGDALVFENERLQQRMNLFFDKIEAQLRQCLR
                     PAGEAVAAPTLQAQVRAAALCDLIRGRLQRYARTGFRRLPSEQLDATLALLL"
     gene            1190..1837
                     /locus_tag="PK518_16005"
     CDS             1190..1837
                     /locus_tag="PK518_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM50461.1"
                     /translation="MTLPITPRKTAAAPRREGRAMHKGQQTKAAIVDAALGLATHIGL
                     EGLSIGALADVTGMSKSGVFAHFGSREELQISVIREYHQRFEHEVFFPAMDAPRGVAR
                     LRAMFGNWMQRTSIEIDSGCIYISGAVEFDDRDGPVRDALAHSVLTWQAAMKRAIMQC
                     KDSGEIRRDVAEDQMLFEIHGLILALHYEARFLHTPGSIDRANKGFDNILARYAA"
BASE COUNT          340 a          589 c          597 g          346 t
ORIGIN      
        1 ccatgctgct cgaaattctg accgaccagg cctacggcac gatgatccgc agcagctgat
       61 agggctgcca gcggttgatc cggcttggtt attggctttg taggagcggc ttcagccgcg
      121 aatggcgcca ttcgcggctg aagccgctcc tacgcacaga tggcatcggc tgctgtcaga
      181 tcagtattgt gcagatggtg aaggttgcag atttggaaca aaagttgcaa cttagccacg
      241 tacgcatatc cacccatggg catttcgacg ggctgtgcga gaatcaattg cacacatttg
      301 tttcgatatg tctgaattca cgcctaccta tcccgagtcc tccaagcccg acgaggcgcg
      361 cgtcacgcgc cgtcgcctgc agcccggtga gaggcgcgag cagattttgc aggccctggc
      421 ggccatgctg cagcagccgg gggccgagcg tgtcaccacg gccgccctgg cgcgccagct
      481 gggggtgagc gaggcggcgc tgtaccgcca tttcgccagc aaggcgcaga tgttcgaagg
      541 gctgatcgac ttcatagagc acagcgtatt caccctggtg cgccagatcg aggagcaggg
      601 tgaccaggcc aatggggcct tgcgcgccgc gcgcatcgcg gccatggtgc tgcagtttgg
      661 cgagcacaac ccgggcatgg tgcgcgtcat ggtgggcgat gcactggtgt tcgagaacga
      721 gcgcctgcag cagcgcatga acctgttctt cgacaagatc gaggcccagc tgcgccagtg
      781 cctgcgcccg gccggcgagg ccgtagccgc gcccacgctg caggcgcagg tgcgcgcagc
      841 ggcgctgtgc gacctgatcc gcgggcggct gcaacgttac gcgcgtacgg gctttcgccg
      901 actgccgagc gagcagttgg acgctacatt ggcgctgttg ctgtagggac ttgcatattc
      961 ccgcctggct ggccatatcg actagatagc gtgcggtctc tggctgggcc gcaagccctc
     1021 acccccaccc tctcccagag gcaggggggt aatgtcgggg caaccggcag ccctcgcacc
     1081 tcgcgggtga ctagtggttt acccgtaaat acactgctca accaaccgag gactggcaca
     1141 agtccgcagt tttgtgcaaa atagaacgat cgttcgtttt atttttccca tgaccctacc
     1201 catcacccca cgcaagactg ccgccgcgcc caggcgcgaa gggcgggcca tgcacaaagg
     1261 ccagcagacc aaggcggcca tcgttgatgc cgcgctgggc ctggccacgc atatcgggct
     1321 ggaggggttg tccataggcg cgctggccga tgtgacgggc atgagcaagt cgggcgtgtt
     1381 tgcccatttc ggctcgcgcg aggagttgca gatctcggtg atccgcgaat accaccagcg
     1441 ttttgagcac gaggtgttct tccctgccat ggatgcgccg cgcggcgttg cgcgcctgcg
     1501 cgccatgttt ggcaactgga tgcagcgcac ctcgatagag atcgattcgg gctgcatcta
     1561 catcagcggc gccgtggagt ttgacgaccg cgacggcccg gtgcgcgatg cgctggccca
     1621 ttcggtgctg acctggcagg ccgccatgaa gcgcgccatc atgcagtgca aggacagcgg
     1681 cgagatccgc cgggacgtgg cggaagacca gatgctgttc gagatccatg gcctgatcct
     1741 ggcgctgcac tacgaggcgc gctttctgca cacccccggc tcgatagacc gcgccaacaa
     1801 gggcttcgac aatatcctgg cgcgctacgc cgcctgatgc cgtcgcccac tcaccactta
     1861 ccactcacca ct
//
LOCUS       DAOMTQ010000297         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_19404_length_1865_cov_20.677348, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000297 DAOMTQ010000000
VERSION     DAOMTQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19404_length_1865_cov_20.677348"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK518_16010"
     CDS             <1..429
                     /locus_tag="PK518_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50462.1"
                     /translation="QAALAAMRPQRAAPKIERLTQYDDAYYARGQGAEARLRRPLPVW
                     RAHWPEDGITVYADPAAARIALQTGSRAPYQRWLYHGLHSLDFAPLLARPLLRDALVL
                     GLSLLGLGLCATSCVLAWRVLAPAPRQRPLARATMRPRQS"
     gene            433..960
                     /locus_tag="PK518_16015"
     CDS             433..960
                     /locus_tag="PK518_16015"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRM50463.1"
                     /translation="MTLRSCTLSTPLGDMLAVASPAGLCLLEFVGQKGVERELAQVEA
                     VRGSLAQMGDSPILAQARAELAEYFAGQRQGFGVPLDLVGTTFQQAVWRALLAIPHGQ
                     TRSYAQQAASIGQPSATRAVAAANGANKVSIIVPCHRVIGSNGSLTGFGGGLARKRAL
                     LALEGGGAQAELYDH"
     gene            950..1657
                     /gene="bluB"
                     /locus_tag="PK518_16020"
     CDS             950..1657
                     /gene="bluB"
                     /locus_tag="PK518_16020"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HRM50464.1"
                     /translation="MTTEPLPAQASPAQAYSAAERAALYRAIDERRDMRHFAGGSVAP
                     ELLTRLLTAAHHAPSVGFMQPWRFIRVRSCALREQLHACVEVERQRTADALGGRRDGF
                     LQLKVEGLLDAAEVFAVALADGREAHVFGRRTLPEMDLASVACAIQNLWLAARAEGLG
                     MGWVSIFDPADVASLLQLPPGARAVALLCLGPVHGFYEEPMLQRERWARRAPLESVVF
                     DEGWGCRADWLTQAPGG"
     gene            complement(1662..>1865)
                     /locus_tag="PK518_16025"
     CDS             complement(1662..>1865)
                     /locus_tag="PK518_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM50465.1"
                     /translation="AERRFDMPVQFSDFAEFDQRMMRPSFADHRIDAALQARVAAAFA
                     PHCTAEGARFVRPMHVRLLRRKG"
BASE COUNT          244 a          685 c          662 g          274 t
ORIGIN      
        1 caggcggcgc tggccgccat gcgcccgcag cgggccgcgc cgaagatcga gcgcctgacg
       61 cagtacgacg acgcctacta cgcgcgcggc cagggcgccg aggcgcggct gcgccgcccg
      121 ctgccggtgt ggcgcgcgca ttggccagag gacggcatca ccgtgtatgc cgacccggcg
      181 gcggcgcgca tcgccctgca gaccggcagc cgcgcgccct accagcgctg gctctaccat
      241 ggcttgcaca gcctggactt tgccccgctg ctggcgcgcc cgctgctgcg cgatgcgctg
      301 gtgctggggc tgtcgctctt gggcctgggg ctgtgcgcca cgtcctgcgt gctggcctgg
      361 cgcgtgctgg cgccggcgcc acggcagcga ccgctggcgc gggcgacaat gcggccccgg
      421 caaagctgaa ccatgaccct gcgctcctgc accctctcca cgcccctggg cgacatgctg
      481 gccgtggcct cgccggccgg cctgtgcctg ctcgaattcg tcggccaaaa gggcgtggag
      541 cgcgagctgg cccaggtcga ggccgtgcgc ggcagcctgg cgcagatggg tgacagcccc
      601 atcctggcgc aggcccgcgc cgagctggcc gaatactttg ccgggcagcg ccagggcttt
      661 ggcgtgccgc tggatctggt cggcacgacc ttccagcagg cggtctggcg ggcgctcttg
      721 gccatacccc atggccagac gcgcagctac gcccagcagg ccgcgtccat aggccagccc
      781 agcgccacgc gcgccgtggc cgccgccaac ggcgccaaca aggtgtccat catcgtcccc
      841 tgccaccgcg tcatcggcag caacggcagc cttaccggct ttggcggcgg cctggcgcgc
      901 aagcgcgcgc tgctggcgct tgaaggcggg ggcgcacaag cagaacttta tgaccactga
      961 accccttccc gcccaggcct ctcctgccca ggcctacagc gcggccgagc gcgccgccct
     1021 gtaccgcgcc atcgacgagc gccgcgacat gcgccacttt gccggcggca gcgtggcgcc
     1081 cgagctgctc acgcgcctgc tcaccgccgc gcaccacgcg cccagcgtcg gcttcatgca
     1141 gccctggcgc ttcatccgcg tgcgcagctg cgccttgcgc gagcagctcc atgcttgtgt
     1201 tgaagtagag cgccaacgca ctgcggacgc cctgggcggg cgccgcgacg gttttttgca
     1261 gttgaaggtg gaggggctgc tcgacgccgc cgaggtcttc gccgtggccc tggccgacgg
     1321 gcgtgaggcc catgtgtttg gccgccgcac cctgcccgag atggacctgg cctcggtcgc
     1381 ctgcgccatc cagaacctgt ggctggccgc gcgcgccgag gggctgggga tgggctgggt
     1441 ctccatcttc gacccggccg acgtcgccag cttgctgcaa ctgccgccgg gcgcgcgcgc
     1501 cgtggcgctg ttgtgcctgg ggccggtgca tggcttttac gaggagccca tgctgcagcg
     1561 cgaacgctgg gcacgccgtg cgccgctcga atcggtggtg tttgatgaag gctggggctg
     1621 ccgcgctgat tggctgaccc aggcgccagg cggctgaagg ctcagccctt gcgccgcagc
     1681 agccgcacat gcatggggcg cacaaagcgc gcaccttcgg ccgtgcaatg cggcgcaaag
     1741 gccgcggcca cgcgcgcctg cagggccgcg tcgatgcggt ggtcggcaaa gctggggcgc
     1801 atcatgcgct ggtcaaactc ggcaaagtcg ctgaactgca cgggcatgtc aaaacgccgc
     1861 tcggc
//
LOCUS       DAOMTQ010000298         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_20053_length_1826_cov_22.356861, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000298 DAOMTQ010000000
VERSION     DAOMTQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20053_length_1826_cov_22.356861"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PK518_16030"
     CDS             complement(<1..561)
                     /locus_tag="PK518_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRM50466.1"
                     /translation="MKLPSLQRRTLALIAVIVPLLLLFVYVALRSGPLAPVAVTLTTV
                     QSRAVAPALAGIGTVQVRYLYKIGPTVAGRLQRLDVHVGDRVRAGQLLGEMDAVDLDE
                     RMRAAQAAILSAEAGLRQAQARQIFAQSQARRYEQLVSVRVSSEESYATRQQELAVAD
                     AALAGAREDASRLRAELQALRAQRGNL"
     gene            complement(558..1166)
                     /locus_tag="PK518_16035"
     CDS             complement(558..1166)
                     /locus_tag="PK518_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM50467.1"
                     /translation="MNEQAKYLPADERRSATVEAVVDLAAQQNPSDITTTAIAQHMGL
                     TQGALFRHFPSKDAILQAVMTWVAERLLLRLDKAAGQAASPTAALQAVFMAHIGFVSE
                     RPGVPRLIFGELQRPGQTVAKQMVQTLIQQYGERLRRLLEAGKAQGELDAALDVPAAA
                     VLFIGTVQGLVMQSLLAGDVARMRQDAPGVFAIYLRGIRRQP"
     gene            1267..>1826
                     /locus_tag="PK518_16040"
     CDS             1267..>1826
                     /locus_tag="PK518_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50468.1"
                     /translation="MTSLAVTALQYRQNPPDVEGQGPFRYRLLAEGDSWMDKSGPALG
                     ALPQFLAEEMNRQRQSVLIINISTAGDTLRRITHVMQGEFGWWLDQFRYDGILLSAGG
                     NDFIDAARDPGPGRGILRDMAGQALPGDGYDCVNQQALAKLIGRYLKPNFATVYGAIR
                     SHPLNAATPIFLNSYDTPTARNAPAAP"
BASE COUNT          292 a          669 c          587 g          278 t
ORIGIN      
        1 caggttgccg cgctgggcgc gcagcgcctg caactcggcg cgcaggcggc tggcgtcctc
       61 gcgcgcgccg gccagggcgg catcggccac ggccagctcc tgctggcgcg tggcgtagct
      121 ttcctcgctg gacacacgca ccgacaccag ctgctcgtag cgccgcgcct ggctctgcgc
      181 aaagatttgc cgggcctggg cctggcgcag gccggcctcg gcgctcagga tggcggcctg
      241 ggcggcgcgc atgcgctcgt ccaagtccac cgcgtccatc tctcccagca gctggccggc
      301 gcgcacccgg tcgcccacat gcacgtccag gcgctgcagg cgcccggcca ccgtcgggcc
      361 tatcttgtag aggtagcgca cctgcaccgt gcctatgccc gccagcgccg gcgccacggc
      421 gcgcgactgc accgtggtca gcgtcacggc cacgggcgcc agcggcccgg agcgcagcgc
      481 cacatacaca aacagcagca gcaaaggcac gatgacggca atcaacgcca gcgtgcggcg
      541 ctgcaggctg ggcagcttca tggctgcctc ctgatgccgc gcaggtagat ggcgaagacc
      601 cccggcgcgt cctggcgcat gcgcgccacg tccccggcca gcagcgactg catcaccagc
      661 ccctgcaccg tgcctatgaa gagcacggcg gcggccggca cgtccagcgc cgcgtccagc
      721 tcgccctggg ccttgccggc ctccagcagc cggcgcaggc gctcgccgta ctgctgaatc
      781 agcgtctgca ccatctgctt ggctaccgtc tggcccggcc gctgcagttc gccaaagatc
      841 aggcgcggca cgcccgggcg ctcgctgacg aaaccgatat gcgccatgaa caccgcctgc
      901 aaggccgccg tgggcgacgc cgcctgcccc gccgccttgt ccaggcgcag cagcaggcgc
      961 tcggccaccc aggtcatcac cgcctgcagg atggcgtcct tgctcgggaa atgccggaac
     1021 agcgcgccct gcgtcaaccc catgtgctgg gcgatggccg tggtcgtgat gtcgctgggg
     1081 ttttgctgcg cggccagatc gaccaccgcc tccaccgtgg cgctgcgccg ctcatcggcc
     1141 ggcaggtatt tggcttgttc gttcatgatc gctcagcgaa agaaagtaat ttattactat
     1201 cttatcgaca aaaccgtgcg atgcaaccat gatgcagatt cgcgcacatc accaaagagg
     1261 cttgccatga cctcccttgc cgtcacagcc ctccagtacc ggcagaaccc gcccgatgtc
     1321 gagggccagg ggccgtttcg ctaccggctg ctggccgagg gcgattcatg gatggacaag
     1381 agcgggccgg ccctgggcgc cctgccccag tttttggccg aggagatgaa ccggcaaagg
     1441 cagtccgtgc tcatcatcaa catctccacg gcgggcgaca ccctgcgccg catcacgcat
     1501 gtgatgcaag gcgagttcgg ctggtggctg gatcagttcc gctacgacgg cattctgttg
     1561 agtgctggcg gcaacgattt catcgacgcg gcgcgcgacc ccggcccggg ccggggcatc
     1621 ctgcgcgaca tggcgggcca ggctctgccc ggcgatggct atgactgcgt gaaccagcag
     1681 gcgctggcca agctgatcgg tcgctacctg aagccgaact ttgccaccgt ctacggcgcc
     1741 atccgcagcc acccgctgaa tgcggccacg ccgattttcc tcaacagcta cgacacgccg
     1801 acggcgcgca acgcgccggc cgcgcc
//
LOCUS       DAOMTQ010000299         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_20327_length_1808_cov_22.643468, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000299 DAOMTQ010000000
VERSION     DAOMTQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20327_length_1808_cov_22.643468"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PK518_16045"
     CDS             <1..1122
                     /locus_tag="PK518_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRM50469.1"
                     /translation="SHALGQRILQLEKEAYDIAGQPFNLGSPKQLGEIFFDKLGMPVV
                     KKTATGARSTDEEVLEKLAEDYPLPAKLLEHRSLAKLKSTYTDKLSQLADPRTGRVHT
                     HYAQAVAVTGRLSSNDPNLQNIPVRTAEGRRIREAFIAPPGRVIASCDYSQIELRIMA
                     HISGDEALLRAFHDGLDVHRATASEVFGVAVDQVSSEQRRYAKTINFGLIYGMSSYGL
                     AKSLGIDNQAAKNYIDRYFQRYPGVWQYMQETKAQAKARGYVETVFGRRLVLPEINSP
                     NGPRRAAAERAAINAPMQGTAADLIKKAMVAVQQVLDAQKPGVLMIMQVHDELVFELP
                     EAEVDWLRLEVPRLMAGVAQLKVPLLAEVGVGPNWEQAH"
     gene            1119..>1808
                     /locus_tag="PK518_16050"
     CDS             1119..>1808
                     /locus_tag="PK518_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRM50470.1"
                     /translation="MRHSEAAPRLTPAVRMGLSIALATGLYGISFGALAVAAGLTVAQ
                     AMALSLLMFTGGSQFAFIGVIAGGGGGAAALSAATLLGVRNGVYGMQMQQLFAPRGWR
                     RAVMAQITIDESAATALGQAEPDEQRRGFWTAGVGVFVLWNAFTLLGALLGDALGDPR
                     RFGLDGAAVAAFLGLLWPRLQGREPIALALACGLVTALAIPQVPPGLPILLAAAVGAA
                     WGWLRPAPPAAG"
BASE COUNT          280 a          646 c          626 g          256 t
ORIGIN      
        1 agccacgccc tgggccagcg catcctgcag ctcgaaaaag aggcctacga catcgccggc
       61 cagccgttca acctcgggtc gcccaagcag ctgggcgaga tcttcttcga caagctgggc
      121 atgcccgtcg tcaagaagac cgccaccggc gcgcgcagca ccgacgaaga ggtgctggaa
      181 aaactggccg aggactaccc cctgccggcc aagctgctgg agcaccgctc cctcgccaag
      241 ctcaagagca cctacaccga caagctcagt caacttgccg acccgcgcac cggccgcgtg
      301 cacacccact acgcccaggc cgtggccgtc accgggcgcc tgtccagcaa cgacccgaac
      361 ctgcagaaca tccccgtgcg cacggccgag ggccggcgca ttcgcgaggc cttcatcgcc
      421 ccgccgggcc gcgtcatcgc cagctgcgac tacagccaga tcgagctgcg catcatggcc
      481 cacatcagcg gcgacgaagc cctgctgcgc gccttccacg acggcctgga cgtgcaccgc
      541 gccaccgcat cggaagtgtt cggcgtggcc gtcgatcaag tgtcaagcga gcagcgccgc
      601 tacgccaaga ccatcaactt cggcctgatc tacggcatga gcagctacgg cctggccaag
      661 agccttggca tagacaacca ggccgccaag aactacatag accgctactt ccagcgctac
      721 cccggcgtgt ggcaatacat gcaggaaacc aaggcccagg ccaaggcgcg cggctatgtc
      781 gagaccgtgt tcggccggcg cctggtgctg cccgagatca actcccctaa cggcccgcgt
      841 cgcgccgcgg ccgagcgcgc cgccatcaac gcccccatgc agggcacggc cgccgacctg
      901 atcaagaagg ccatggtggc cgtgcagcag gtgctggacg cgcaaaagcc cggggtgctg
      961 atgatcatgc aggtgcacga cgaactggtg ttcgagctgc ccgaagccga ggtggactgg
     1021 ctgcgcctgg aggtgccgcg cctgatggcc ggcgtggcgc agctgaaagt gccgctgctg
     1081 gccgaggtgg gcgtggggcc gaattgggag caggcgcatt gaggcatagc gaagcggcgc
     1141 cgcgcctcac ccccgccgtg cgcatgggcc tgtccatcgc cctggccacg gggctgtacg
     1201 gcatctcgtt tggcgccctg gccgtggccg cgggcctgac cgtggcccag gccatggcgc
     1261 tcagcctgct aatgtttacc ggcggctcgc agttcgcctt catcggcgtg attgccgggg
     1321 gcggcggagg tgcggcggcg ctgagcgcgg cgaccctgct gggggtgcgc aatggcgtct
     1381 atggcatgca gatgcagcag ctgtttgccc cgcgcggctg gcgccgggcg gtgatggcgc
     1441 agatcaccat cgacgagtcg gccgccacgg ccctgggcca ggcagagccc gacgagcagc
     1501 gccgcggctt ctggactgcc ggcgtcggcg tcttcgtgct gtggaacgcc ttcacgctgc
     1561 tgggcgcgct gctgggcgac gcgctgggtg acccgcgccg cttcgggctg gacggcgcgg
     1621 ccgtggcggc ctttctgggc ctgttgtggc cgcgcctgca ggggcgcgag cccatcgcgc
     1681 tggcgctggc ctgcggcctg gtgacggcgc tggccattcc gcaggtgccg ccgggcctgc
     1741 ccatcctgct ggcagccgcc gtcggcgcgg cctggggctg gctgcggccc gcgccgcctg
     1801 cggccggg
//
LOCUS       DAOMTQ010000300         1793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_20593_length_1793_cov_17.809551, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000300 DAOMTQ010000000
VERSION     DAOMTQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20593_length_1793_cov_17.809551"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..93
                     /locus_tag="PK518_16055"
     tRNA            17..93
                     /locus_tag="PK518_16055"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Ile,seq:cat)
     gene            185..1306
                     /locus_tag="PK518_16060"
     CDS             185..1306
                     /locus_tag="PK518_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRM50471.1"
                     /translation="MPSHTAYAAHAAEQAGAAAQYTSVAPALYRAALGAVNPDHYLAA
                     FERFDGVGRVLPGWNLAAALCPLGWLVFRRLWRHALVLALGLVAGALLLWAGHRWLAV
                     PLPMLGGVALAGLLMLGAGLGLYGDALVHADVHRRMTAAVSAAPNMREAVALLQRQAA
                     SWRRLWWLVASAAAGLVLVVVAAWWTFAGRVTAQPVQDAVAVTVVATARPESVPQPSQ
                     PALPSAAVMESLPDPAGEPGMAEPDPWPAAVVRTPAARPPSAQAVPESAPVAAAPQAT
                     SEPRAQAVAPVEERAPASRAQRRLYVNVGLFADAENARRAYLRLRQAGLSATIDPLVR
                     ADGSRLQRVRVGPFTSAAQANQAAARVRALGLDAVAAAQ"
     gene            1407..1733
                     /locus_tag="PK518_16065"
     CDS             1407..1733
                     /locus_tag="PK518_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HRM50472.1"
                     /translation="MWNSLRIAWLTGLVAVWLLGGCSQLGPASAPVPIGASTAPVGGT
                     CNVQAAQWAVGKSGTARVVEEARVRSGARMARVVHPGTAAQAFDAQRLNLEVDGNGTI
                     TAVRCG"
BASE COUNT          232 a          549 c          682 g          330 t
ORIGIN      
        1 gcgtcttgcg caccctgggg cgttagctca gttggttaga gcagaggact cataatcctt
       61 tggtcgtagg ttcaagtcct acacgcccta ccagtccaga aaacccgcca cgacttcgtt
      121 tgtggcgggt ttttgctttt ttgctcctgg gcgggcgcat ggcctgcatg ccagaatcgc
      181 gcgcatgcca agccacaccg cctatgccgc acatgccgcc gaacaggctg gcgcggccgc
      241 gcagtacacc agcgtcgcac cggcgctgta tcgtgcagcg ctgggcgcag tgaatcctga
      301 tcactacctg gcagcgtttg aacgcttcga tggggtgggg cgggtgctgc ccgggtggaa
      361 tctggcggcg gccctgtgcc cgctgggatg gctggttttc aggcggctgt ggcgccatgc
      421 gttggtgctg gcgctgggtc tggtggcggg cgcgttgctg ctgtgggccg ggcatcgatg
      481 gctggccgtg cccctgccca tgctgggcgg cgtggccctg gcgggcttgc tgatgctggg
      541 cgccgggctg ggcttgtatg gcgacgcact ggtgcatgcc gacgtgcaca ggcgcatgac
      601 cgccgccgtg tctgccgcgc ccaacatgcg cgaggccgtg gccttgctgc agcgccaggc
      661 tgccagctgg cgcaggcttt ggtggttggt ggcgtcagcg gcggcagggc tggtgctggt
      721 cgttgttgcc gcgtggtgga cgtttgcggg ccgtgtgacg gcccaaccgg tgcaagacgc
      781 ggtggccgtg acggtcgtgg cgacggccag gcccgagtcg gtgccgcaac cctcgcagcc
      841 ggcgctgcca tcggctgcgg tgatggagtc cttgcccgat ccggccggag aacccggcat
      901 ggcggagcct gatccatggc ccgcggccgt tgttcggacg ccggccgcac ggccgccatc
      961 ggcgcaagcg gtgccggaat ccgcacctgt tgccgctgcg ccgcaagcca cgtccgagcc
     1021 gcgtgcccaa gccgtcgcac cagtcgaaga gcgcgcgcct gccagcaggg cgcagcggcg
     1081 gctgtatgtc aacgtggggc tgtttgccga cgctgaaaac gcgcgccgcg cctacctgcg
     1141 cttgcggcag gcgggcttgt cggccaccat cgacccgctg gtgcgggccg acggcagccg
     1201 cttgcagcgt gtgcgcgtgg ggccgtttac ctcggccgcg caggccaatc aggctgcggc
     1261 gcgtgtgcgt gcgctggggc tcgatgccgt ggcggccgcg cagtgactgc gctcgggctt
     1321 gaacagggat tggaacaaca gcggcagtgc tgcgccgggc gcgtcacctg gggtatgggg
     1381 gtttcttttt tctggaagga ctgaacatgt ggaattcatt gcgcattgcc tggctgacgg
     1441 gcctggtggc tgtgtggctg ctgggcgggt gttcgcaact cgggccggcc agtgcaccgg
     1501 tgcccattgg cgccagcacc gcgcccgtgg ggggcacctg caatgtccag gcagcgcagt
     1561 gggccgtggg caaaagcggc acggccaggg tggtggaaga ggcgcgcgtg cggtctggcg
     1621 cgcgcatggc gcgcgtggtg catccgggga cagcggctca ggcgtttgat gcccagaggt
     1681 tgaacctgga ggtggatggc aacggcacga ttacggccgt gcgctgcggt tgatactagt
     1741 gcagtgttcg acgctatctg gaacataaaa ccgcgccttt tcggcctggg cgg
//
LOCUS       DAOMTQ010000301         1760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_21174_length_1760_cov_17.245748, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000301 DAOMTQ010000000
VERSION     DAOMTQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21174_length_1760_cov_17.245748"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1548)
                     /locus_tag="PK518_16070"
     CDS             complement(235..1548)
                     /locus_tag="PK518_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HRM50473.1"
                     /translation="MPWLAWLLPCIDPAPGVPPDAQGWWALRFTPCVTLLDEALLLEV
                     DSVERLWGGRAALQALLRNQAPAAAQENAADQAWATGPTALQALALLRLQRMGRSPPQ
                     RLPHDLPIATLTALRPHVAALQHLGCRSWGDLRALPRAGLARRFGAACLLALDQAWGD
                     APHGLIWITLPERFALELELSALVESAPALLMAGQRLLDALQGWLRARQQGVLALELA
                     WRHDLRRVDGVDLPPWQSLELRTAEPLQAMAHLRRLLAERLAHQRLAAPVNRLALRSL
                     QTAPLAQASASLLPPAPDEARGEPWHQLIERLSARLGPASLQVARLHADHRPERMQHW
                     HPAITPPSPAQDEAADSPAGLWPPWLVRPPQPLDLQGHRPCLLGRPLRLLAGPQRVEA
                     GWWEGESGTGLARRDYFLAHNAAAGSVWVFRDRATGGWFLQGVYG"
     gene            complement(1514..>1760)
                     /locus_tag="PK518_16075"
     CDS             complement(1514..>1760)
                     /locus_tag="PK518_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50474.1"
                     /translation="ASPAPLRLWLQPQADRLLIHVIKRRGPSALHPVALPGHAALLAA
                     VLQGQAQRRQQAQAATWGRARPQEVAHAVAGLAAALH"
BASE COUNT          252 a          650 c          644 g          214 t
ORIGIN      
        1 ctagaaacag cagttgaccg ctttcaaact tcacgagaac cgcatgggta ttgcctttag
       61 ctgcctcgtt gccgctcacc tgcggggtga cagcgctacg cacttgccag caccgcgctc
      121 gccgcgccat gcggcgttgc tcctccttgc gggcagtagc ccgccgcgtc gtcacgcctt
      181 gcctgacacg ccgatcacgg cactggcaag cgcgtccaac tgccgtttct aggatcaccc
      241 gtacaccccc tgcagaaacc agccgcccgt ggcccggtcg cgaaacaccc agacgctgcc
      301 ggcggcggcg ttgtgcgcca gaaagtaatc gcgccgggcc aggccggtgc ctgactcacc
      361 ctcccaccaa ccggcctcca cacgctgcgg cccggcaagc aggcgcaagg ggcggcccaa
      421 caggcagggg cgatggcctt gcaggtccag gggctgcggt ggacgcacca gccagggggg
      481 ccacagtcca gcggggctgt cggccgcctc gtcctgggca ggagacggcg gcgtgatggc
      541 ggggtgccag tgctgcatgc gctctggccg gtggtcggca tgcagccgcg ccacctgcag
      601 gctggccggg cccagccggg ccgacaggcg ctcgatgagc tggtgccagg gctcgccccg
      661 ggcctcgtcg ggcgcgggcg gcagcaggct ggcgctggcc tgggccaggg gcgccgtctg
      721 caggctgcgc agcgccaggc ggtttaccgg cgcggccagg cgctggtgcg ccagtcgctc
      781 ggccagcagg cggcgcaggt gggccatggc ctgcagcggc tcggcggtgc gcagctccag
      841 gctttgccag ggcggcaagt ccacgccatc gacgcggcgc aggtcatggc gccaggccag
      901 ctccagcgcc agcacaccct gctggcgcgc acgcagccag ccctgcagcg catccagcag
      961 gcgctggccg gccatcagca aagccggggc cgattcgacc agggccgaca actccagctc
     1021 cagcgcaaac cgttcgggca gggtgatcca gatgaggcca tgcggggcgt cgccccaggc
     1081 ctgatccagc gccagcaggc aggcggcacc aaagcgccgc gccaggccgg cacgcggcag
     1141 cgcgcgcaaa tcgccccagc tgcggcagcc caggtgctgc agcgccgcca catgcggacg
     1201 cagtgccgtc agggtggcga tgggcaggtc gtgcggcagc cgctgcggcg ggctgcggcc
     1261 catgcgctgc aggcgcagca aggccagggc ctgcagggcc gtggggccgg tggcccaggc
     1321 ttgatcggcc gcgttctctt gcgctgctgc gggcgcctgg ttgcgcagca gcgcctgcag
     1381 ggcggcgcgc ccgccccaca ggcgctccac gctgtcgacc tccagcagca atgcttcgtc
     1441 cagcaaggtc acgcacggcg tgaagcgcag cgcccaccag ccctgggcgt ctggcggcac
     1501 accgggcgcc gggtcaatgc agggcagcag ccaggccagc cacggcatgt gccacctcct
     1561 gcggcctggc cctgccccag gtggcggcct gcgcctgctg gcgccgctgc gcctggccct
     1621 gcaggacggc ggccagcagc gcagcatgcc cgggcagtgc cacggggtgc agcgcgctcg
     1681 ggccgcggcg cttgatgacg tggatcagca gccgatccgc ctgcggctgc agccacaggc
     1741 gcaggggcgc gggcgacgcc
//
LOCUS       DAOMTQ010000302         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_21766_length_1730_cov_24.989254, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000302 DAOMTQ010000000
VERSION     DAOMTQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21766_length_1730_cov_24.989254"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PK518_16080"
     CDS             <1..812
                     /locus_tag="PK518_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRM50475.1"
                     /translation="TAVLNNLEFDHADIFDDLAAIERQFHHLVRTVPPSGRVVFNGLE
                     ESLARVLHAGCWSAVASFGAAVSDFSARGEPHDFEVLQRGEPVARVQWGLSGTHNQLN
                     ALAAIAAAEHVGVAPDQAAQALARFENVKRRMELRGQVRGISVYDDFAHHPTALRTTL
                     DGLRRRVGPEARILAAFEPRSNTMKLGTMKAQLPWALEAADLAFCHTAGLDWDAAEAL
                     APMGARASTAADVDQLVTDLVAQARPGDHIVCMSNGSFGGIHAKLLNKLSN"
     gene            complement(873..1181)
                     /locus_tag="PK518_16085"
     CDS             complement(873..1181)
                     /locus_tag="PK518_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50476.1"
                     /translation="MQSEVLVTLSPGQEFRLSTEGPTLSNEQARRWLDDEFTRLDCEP
                     LRASGKVLLADKVLTVAQAAGATLLADREWSSQFARAAVAALTRPVVRVDVAAMAVTF
                     "
     gene            complement(1224..>1730)
                     /locus_tag="PK518_16090"
     CDS             complement(1224..>1730)
                     /locus_tag="PK518_16090"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM50477.1"
                     /translation="GTDGNIRIATDAIQSASRGHHFLSVHKNGQVAIVRTAGNKDCHV
                     ILRGGKAPNYDAESVAIACKDLAAAGLPASLMVDCSHANSSKQHERQRDVAHDIAGQI
                     AGGSRSVFGVMIEGHLQAGAQKFTPGKDDPAKLEYGKSITDACLGWDDSVACLAELAA
                     AVQARRSV"
BASE COUNT          273 a          572 c          595 g          290 t
ORIGIN      
        1 gcacggccgt gctgaacaac ctggagttcg atcacgccga catctttgac gatctggcgg
       61 cgatcgagcg gcaattccac catctggtgc gcaccgtgcc gccctcgggc cgggtggtgt
      121 tcaacgggct ggaggaaagc ctggcgcgcg tgctgcacgc cggctgctgg agcgcggtag
      181 ccagctttgg ggcggccgtg agcgacttca gcgcacgcgg cgagccgcat gatttcgagg
      241 tgctgcagcg cggcgaaccg gtggcgcgtg tgcagtgggg gttgagcggc acacacaacc
      301 agctcaatgc cctggcggcg attgctgccg ccgagcatgt gggcgtggcc cccgaccagg
      361 cggcccaggc gctggcgcgc ttcgagaacg tgaagcgccg catggagctg cgcggccagg
      421 tgcgcggcat cagcgtctat gacgactttg cccaccaccc gacggccctg cgcaccacgc
      481 tcgatggcct gcgccggcgc gtgggccccg aggcgcgcat cctcgcggcc ttcgagccgc
      541 gcagcaacac catgaagctg ggcaccatga aggcgcagct gccctgggcc ctggaggcgg
      601 ccgacctggc cttttgccac accgccgggc tggactggga tgccgccgaa gccctggcgc
      661 cgatgggcgc gcgggccagc acggcggcag acgttgacca actggtgacc gacctggtgg
      721 cgcaggcgcg accgggcgac cacatcgtct gcatgagcaa tggcagcttt ggcggcatac
      781 acgccaagct gctcaataaa ctatcaaatt aatagctgtt ggcgcttatc catcaagcgc
      841 aaacagccta tttcatccga attttcagag tgtcagaacg tgacggccat ggccgccacg
      901 tccacccgca ccacggggcg ggtcagcgcc gccacggcgg cccgggcaaa ctggctggac
      961 cactcgcggt cggccagcag cgtggcaccc gcggcctggg ccacggtgag caccttgtcg
     1021 gccagcaaca ccttgccgct ggcgcgcagg ggttcgcagt ccaggcgcgt gaactcgtca
     1081 tccagccagc ggcgggcttg ctcgttgctg agggttgggc cttcggtact caggcggaat
     1141 tcctgtccgg gcgagagggt gaccaatact tcgctctgca tagctcgtct ttcttgagat
     1201 gggtggtgat gtgccagtgc agcttacacc gaacggcgcg cctgcacggc ggccgccagc
     1261 tcggccaggc aggccacgga atcgtcccaa cccaggcagg catcggtgat gctcttgccg
     1321 tactccagct ttgcagggtc gtccttgccc ggtgtgaact tctgcgcgcc ggcctgcaga
     1381 tggccctcga tcatgacgcc gaacacgctg cgcgagccgc cggcgatctg gccggcgatg
     1441 tcatgcgcca cgtcgcgctg gcgctcatgc tgcttgctgc tgttggcgtg gctgcagtcc
     1501 accatcagcg aagccggcag acccgcagcc gccagatcct tgcaggcaat ggccacgctc
     1561 tcggcgtcgt agttgggcgc cttgccaccg cgcaggatga catggcaatc cttgttgccg
     1621 gcggtgcgca cgatggccac ctggccgttc ttgtgcaccg acaggaaatg gtggccgcgg
     1681 ctggcggact ggatggcgtc ggtggcgatg cggatgttgc cgtcggtgcc
//
LOCUS       DAOMTQ010000303         1718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_21982_length_1718_cov_13.912808, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000303 DAOMTQ010000000
VERSION     DAOMTQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21982_length_1718_cov_13.912808"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PK518_16095"
     CDS             <1..679
                     /locus_tag="PK518_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRM50478.1"
                     /translation="LHAGAGRVLLALLDDGASSALPELPELMLRRVEALRLEQGAVVC
                     GCGGGAAVAARLPAVLQQAQRLVLDADALNAIAADAELQRLLSERGRHADCATVLTPH
                     PLEAARLLATSAAAVQADRLAAAQALADRYQCTVLLKGSGSIIAAPGQLPHINPTGNA
                     LLATGGTGDVLAGLVGARLAAGPAASAAAAFDAAIQACWQHGAVADDWRAPQALTASR
                     LAQSLHP"
     gene            689..1519
                     /locus_tag="PK518_16100"
     CDS             689..1519
                     /locus_tag="PK518_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRM50479.1"
                     /translation="MHPDSNQIRARRATFRQLHAAGCFAIPNPWDAASARWLAGLGFQ
                     ALASTSAGHAWSQGHADGGMPLPAVLDYLRSLVAATDLPVNADFEHGFADDAAGVARN
                     VALAVDTGVAGLSIEDSTGRPDAPLLPLDEAVQRVRAARAAIDASGQDVLLVGRAENF
                     FVGRPDLDDAIGRLRAYAEAGADCLYAPGIHTPEQIAAVVAAVAPKPVNVLVGSASTL
                     TMADLAALGVRRVSVGGGLARAAWGGFDRAARLLANEGRFDGFADALPGQRLNQFFGA
                     "
     gene            complement(1501..>1718)
                     /locus_tag="PK518_16105"
     CDS             complement(1501..>1718)
                     /locus_tag="PK518_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM50480.1"
                     /translation="ALRAGRATEATADLAISTASQQGWRRPLLAWLLLRVAQAQAAGD
                     TVLAAALQRRIALIESGGAPAAQAPKN"
BASE COUNT          210 a          663 c          619 g          226 t
ORIGIN      
        1 gctgcacgcg ggcgcggggc gcgtgctgct ggcactgctc gatgacggcg ccagcagcgc
       61 cctgcccgag ctgccggagc tgatgctgcg ccgcgtggag gccctgcgcc tggaacaggg
      121 cgccgtggtc tgcggttgcg gcggcggcgc tgccgtggcc gcccggctgc ccgccgtgct
      181 gcaacaggcg cagcgcctgg tgctggatgc ggacgctctg aatgccatag ctgccgacgc
      241 agagctgcag cgcctgctga gcgagcgtgg ccgccatgcc gactgcgcca cggtgctcac
      301 cccgcatccg ctggaggccg cgcgcctgct ggccaccagc gccgccgccg tgcaggccga
      361 ccgcctggcc gccgcccagg cactcgccga tcgctaccag tgcacggtgc tgctcaaggg
      421 ctcgggcagc atcattgccg caccgggcca gctgccgcac atcaacccca cgggcaatgc
      481 gctgctggcc accggcggca cgggcgacgt gctcgccggc ctggtgggcg cacgcctggc
      541 cgcgggcccg gccgccagcg ccgcggcagc ctttgatgcc gccatccagg cctgctggca
      601 gcatggcgcc gtggccgatg actggcgcgc gccacaggcc ctgaccgcat cgcgactggc
      661 gcagtccctg cacccataag gaaacagcat gcaccccgac agcaaccaga tccgcgcccg
      721 gcgcgccacc ttccgccagc tgcacgcagc gggctgcttt gccatcccga atccctggga
      781 cgcggcctcg gcgcgctggc tcgccggcct gggctttcag gcgctggcca gcaccagcgc
      841 gggccatgcc tggtcgcaag gccatgccga cggcggcatg ccgctgccgg ccgtgctgga
      901 ctacctgcgc agcctggtgg cggcaaccga tctgcccgtc aacgccgact tcgagcatgg
      961 ctttgccgac gatgcggcgg gcgtggcgcg caacgtggcg ctggccgtgg acacgggcgt
     1021 ggccggcctg tccatcgaag actccaccgg ccggcccgat gcgccactgc tgccgctgga
     1081 cgaggcggtg cagcgcgtgc gcgccgcacg cgccgccata gacgccagcg gccaggatgt
     1141 gctgctcgtc ggccgggccg agaacttctt cgtcggccgc ccggatctgg acgacgccat
     1201 tggccgcctg cgtgcctacg ccgaggccgg tgccgactgc ctgtacgccc ccggcatcca
     1261 cacccccgag cagatcgccg ccgtggtggc cgcggtagcg cccaagccgg tgaacgtgct
     1321 ggtgggctcg gccagtaccc tcaccatggc cgacctggcg gccctgggcg tgcgccgcgt
     1381 cagcgtgggc ggcggtctgg cgcgcgccgc ctggggcggc tttgaccgcg ccgcgcgcct
     1441 gctggccaat gaaggccgct ttgacggctt tgccgacgcg ctgccaggcc agcgcctgaa
     1501 tcagttcttt ggcgcctgag cggcgggcgc gccgccgctc tcgatcagcg caatgcgccg
     1561 ctgcaaggcg gccgccagca ccgtatcgcc cgccgcctgg gcctgggcca cgcgcagcag
     1621 cagccaggcc agcagcggcc ggcgccagcc ctgctgcgac gcggtgctga ttgccagatc
     1681 cgccgtggcc tcggtggcgc ggccggcgcg cagcgcag
//
LOCUS       DAOMTQ010000304         1684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_22659_length_1684_cov_28.920196, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000304 DAOMTQ010000000
VERSION     DAOMTQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22659_length_1684_cov_28.920196"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..>1684
                     /locus_tag="PK518_16110"
     CDS             180..>1684
                     /locus_tag="PK518_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM50481.1"
                     /translation="MMQRTTFKLGMVATAMVLAMGSAQAASMRWAGANDILTIDPHAQ
                     NHQTTHAFLQQIYESLVRYDGKYQTEPALATKWTQISPTQVRFELRKGVKFHDGAPFT
                     ADDVVFSLTRAGTAPSNMMSSVQSVKEVKKVDDHTVDLILKGPNPILLRELTEARIMN
                     KAWAEKHNSLKSQDYAGKEESYASRNANGTGPFSLVAWQPDQKLTLKKNPNWWDKPKG
                     NIDEVVFTPIKSAATRSAALISGQVDFVVDPPPQDLARMKADSNLKLIEGAENRTIYL
                     GLDQFRDELPGSGVAGKNPLKDKRVRQALYQAIDSAGLHSRTMRNLSVPAGVMVAPMV
                     HGWSKQLDERAAKYDVEAAKKLLADAGYPQGFTLKLDCPNDRYVNDEAICQAVTAMWT
                     RIGVKTNLQTAPMAQFVSRVMNNDVSAYLFGWGVATFDALYTLDSLMSTKDGKTAAGV
                     YNGGRFSDPKLDAMIQQIKVEMDTPKRDALIHDALKLTKDEYYYLPLHHQIR"
BASE COUNT          353 a          571 c          521 g          239 t
ORIGIN      
        1 gcccagcagt ggatcatcaa cacccgcatg ggcgtgccgc cgcagttcaa cctgcccaag
       61 ttccgctgac ccgcccccgc gtatgaagga cgacggccgc tttagcggcc gttgtctttt
      121 tcggtaccat gttcagccac tacatccggt agtcaaccaa cacacaagga gccagacgga
      181 tgatgcagcg aacaaccttc aaactcggca tggtggcgac ggccatggtg ctggccatgg
      241 gcagcgcgca ggcggccagc atgcgctggg ccggcgccaa tgacattctg accatagacc
      301 cacacgcgca gaaccaccag accacgcacg ccttcctgca gcagatctac gagagcctgg
      361 tgcgctacga cggcaagtac cagaccgagc cggccctggc caccaagtgg acgcagatct
      421 cgcccacgca ggtgcgcttc gagctgcgca agggcgtcaa attccacgac ggcgcgccgt
      481 tcacggccga cgacgtggtg ttctcgctca cgcgcgcggg cacggcgccg tccaacatga
      541 tgtcctcggt gcagagcgtc aaggaggtga agaaggtcga tgaccacacc gtggatctga
      601 tcctcaaggg cccgaacccc atcctgctgc gcgagctgac cgaggcgcgc atcatgaaca
      661 aggcctgggc cgagaagcac aactcgctca agtcgcagga ctacgccggc aaggaggaaa
      721 gctacgcctc gcgcaacgcc aacggcaccg gccccttcag cctggtggcc tggcagccgg
      781 accagaagct cacgctgaag aagaatccga attggtggga caagcccaag ggcaacatcg
      841 acgaggtggt gttcacgccc atcaagtcgg ctgccacgcg ctccgcggcg ctgatctcgg
      901 gccaggtgga cttcgtcgtc gatccgccgc cgcaggatct ggcgcgcatg aaggccgaca
      961 gcaacctgaa gctcatcgag ggcgccgaga accgcaccat ctacctggga ctggaccagt
     1021 tccgcgacga gctgcccggc tcgggcgtgg ccggcaaaaa cccgctcaag gacaagcgcg
     1081 tgcgccaggc gctgtaccag gcgatcgact cggccggcct gcacagccgc accatgcgca
     1141 acctgtcggt gcccgccggc gtgatggtgg cgcccatggt gcatggctgg agcaagcagc
     1201 tggacgagcg cgccgccaag tacgacgtcg aggccgccaa gaagctgctg gccgacgccg
     1261 gctatcccca gggctttacc ctcaagctcg actgcccgaa cgaccgctac gtgaacgacg
     1321 aggccatctg ccaggccgtc acagcgatgt ggacgcgcat cggcgtcaag accaatctgc
     1381 agaccgcgcc gatggcgcag tttgtctcgc gcgtgatgaa caacgacgtc agcgcttacc
     1441 tgttcggctg gggcgtggcc acgtttgatg cgctgtacac gctggactcg ctgatgtcca
     1501 ccaaggacgg caagacggcg gccggcgtct acaacggcgg gcgcttcagc gaccccaagc
     1561 tcgacgccat gatccagcag atcaaggtgg agatggacac gccgaagcgc gacgcgctga
     1621 tccacgacgc gctcaagctg accaaggacg agtactacta cctgccgctg caccaccaga
     1681 tccg
//
LOCUS       DAOMTQ010000305         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_23128_length_1661_cov_25.096513, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000305 DAOMTQ010000000
VERSION     DAOMTQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23128_length_1661_cov_25.096513"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PK518_16115"
     CDS             complement(<1..1250)
                     /locus_tag="PK518_16115"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRM50482.1"
                     /translation="MKLADNKATLSFSNGKPSVELPVYQGSIGPDVIDIRKLYGQTDM
                     FTYDPGFLSTASCQSSITYIDGDKGELLYRGYPIEQLAQKCDYLDTCYLLLNGDLPNE
                     GQRTDFHKLVLKHTMVNEQMQFFLRGFRRDAHPMAVLTGLIGALSAFYHDSTDINNPE
                     HRHISAIRLISKLPTLVAMAYKYGVGQPYMYPRNDLSYAGNFLRMMFGTPCEDYKVNP
                     VLERAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LNMLEHIQANGGIAKVGEFMEQVKDKNSGVKLMGFGHRVYKNYDPRAKLMQETCNEVL
                     AELGLENDPLFKLAKQVEKIALEDDYFVSRKLYPNVDFYSGIVQRAIGIPVSLFTGIF
                     ALARTVGWIAQLNEMLADPEYKIGR"
     gene            complement(1302..1574)
                     /locus_tag="PK518_16120"
     CDS             complement(1302..1574)
                     /locus_tag="PK518_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HRM50483.1"
                     /translation="MADDALLDERDVAILRWRSRRGLVENDLFIERFFERHGAHLTTR
                     QAHAMTLLMNLSDNDLLDLLLKRKEAEGEIATDDVRALLELLRERA"
     gene            complement(1581..>1661)
                     /gene="sdhB"
                     /locus_tag="PK518_16125"
     CDS             complement(1581..>1661)
                     /gene="sdhB"
                     /locus_tag="PK518_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733632.1"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol; the
                     catalytic subunits are similar to fumarate reductase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HRM50484.1"
                     /translation="VDVCPKGLKPAAAISKIKELMVRRAI"
BASE COUNT          266 a          485 c          574 g          336 t
ORIGIN      
        1 cggccgatct tgtactcggg gtcggccagc atttcgttga gctgggcgat ccagcccacg
       61 gtgcgggcca gggcgaagat gccggtgaac aggctgaccg ggatgccgat ggcgcgctgc
      121 acgatgccgg agtagaagtc cacgttcggg tagagcttgc gcgagacgaa gtagtcgtct
      181 tccagggcaa tcttctccac ctgcttggcc agcttgaaca gcgggtcgtt ctccaggccc
      241 agctcggcca gcacctcgtt gcaggtttcc tgcatcagct tggcgcgcgg gtcgtagttc
      301 ttgtacacgc ggtggccaaa gcccatcagc ttcacgccgc tgttcttgtc cttgacctgc
      361 tccatgaact cgccgacctt ggcgatgccg ccattggcct ggatatgctc gagcatgttc
      421 aggcaggcct cgttggcgcc gccatgggcc gggccccaca ggcaggccac gccggcggcg
      481 atggccgcga acgggttcgt gcccgagctg ccgcacaggc gcacggtgga ggtggaggcg
      541 ttctgctcgt ggtcggcgtg caggatgaag atgcgatcca tggcgcgctc aagcaccgga
      601 ttgaccttgt agtcctcgca gggcgtgccg aacatcatgc gtaggaagtt gccggcgtag
      661 gacaggtcgt tgcgcggata catgtagggc tggccgacgc cgtacttgta ggccatggcc
      721 accagcgtgg gcagcttgga gatcaggcgg atggccgaga tgtggcggtg ctcggggttg
      781 ttgatgtcgg tgctgtcgtg atagaaggcc gacagcgcgc cgatcaggcc ggtgagcacg
      841 gccatggggt gggcgtcacg gcgaaagccg cgcaggaaga actgcatctg ctcgttcacc
      901 atggtgtgct taagcaccag cttgtggaag tcggtgcgct ggccctcgtt gggcagatcg
      961 ccgttgagca gcaggtagca ggtatcgagg tagtcgcatt tttgcgccag ctgctcgatg
     1021 gggtaaccgc ggtacagcag ctcgcccttg tcgccgtcga tgtaggtaat ggacgattgg
     1081 caggacgccg tggacaggaa gcccgggtcg taggtgaaca tgtcggtctg gccgtacagc
     1141 ttgcggatgt cgatcacgtc cgggccgatg ctgccctggt acacgggaag ttccacgctc
     1201 ggcttgccgt tgctgaacga tagcgttgct ttgttgtctg ccagtttcat ttccagtttc
     1261 ctcggttgat tactaggtga gaggggcagg gcctggctgc ttcaggcgcg ttcgcgcagc
     1321 agctccagca gggcccgaac atcgtcggtc gcgatctcgc cttcggcctc cttgcgcttg
     1381 agcagcaggt ccagcaggtc gttgtccgac agattcatga gcagcgtcat cgcgtgcgcc
     1441 tggcgtgtcg tcaggtgcgc gccgtggcgc tcaaaaaaac gctcgatgaa caggtcgttc
     1501 tccaccagcc cgcgccggct gcgccagcgc aggatggcca catcccgttc gtcgagcagg
     1561 gcgtcgtcgg ccatcgcggc tcagatggcg cggcgcacca tcagctcctt gatcttgctg
     1621 atggcggcgg cgggcttgag gcccttgggg cacacgtcca c
//
LOCUS       DAOMTQ010000306         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_23972_length_1623_cov_26.863520, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000306 DAOMTQ010000000
VERSION     DAOMTQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23972_length_1623_cov_26.863520"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PK518_16130"
     CDS             <1..341
                     /locus_tag="PK518_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRM50485.1"
                     /translation="SAGGAESAVLQQAALALLGRGGGDLTGDIARQLGLDEIGVKGPQ
                     AGAGADASEAALTLGKRLTKDLYVTYERSLSGVLGTLYIFYDLSRRLQLRGQTGMQSA
                     VDLIYTVRYD"
     gene            455..529
                     /locus_tag="PK518_16135"
     tRNA            455..529
                     /locus_tag="PK518_16135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:488..490,aa:Arg,seq:cct)
     gene            complement(496..1422)
                     /locus_tag="PK518_16140"
     CDS             complement(496..1422)
                     /locus_tag="PK518_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRM50486.1"
                     /translation="MQRLAWLCAALMLVVASLSAFIRQSRSEQGCEPWPQCQGIAAVQ
                     VGATLPEAGAGVAMARMGHRVVASATLLLVLAMVGLALSAGVAMRAEARLALGLLGLV
                     LFLALLGVLAGPSRLPAVTLGNLLAGFAMCALSVRLALMAGHADAARSRPLPSAWLAV
                     ATALAWAQVALGGMVSAGEAGLSCPALGACDWRAGSWQALDPWALPAGDLLPGHAAGA
                     LVHMLHRWSGMALTLVLLVLAWQAWRLGRRAVAAWLVALPLLLVVLGLVQVWLRLPLP
                     LVLVHNTAAALLLALLSGVGLDGPPDRNRTCI"
BASE COUNT          324 a          595 c          524 g          180 t
ORIGIN      
        1 ccagcgccgg cggcgccgag tccgccgtgc tgcagcaggc cgccctggcc ctgctgggcc
       61 gcggcggcgg cgacctgacc ggcgacatcg cgcgccagct gggcctggac gagatcggcg
      121 tgaaaggccc gcaggccggc gccggcgccg acgccagcga agccgccctg accctgggca
      181 agcgcctcac caaggacttg tacgtgacct acgagcgcag cctctcgggc gttctgggca
      241 cgctgtacat cttctacgac ctgtcccggc gcctgcagct gcgcgggcag acgggcatgc
      301 agagcgcggt ggatctgatc tacaccgtgc gctatgactg attttgatga aatcggcccg
      361 cagcgcataa cagacaagcg caagcagcta taaaaaacgc agcagtcaca tcccaccgga
      421 acacccgcgc cgcgccctta caatgcggcc cgtcctcccc gtagttcaat ggatagaacg
      481 agcgcctcct aagcgctaga tacaggttcg attcctgtcg gggggaccat ccagccccac
      541 gccagacaac agcgccagca gcagcgcggc cgccgtgttg tgcaccagta ccaggggcag
      601 cggcaggcgc agccagacct gcaccagccc caacaccacc agcagcaacg gcaacgcaac
      661 cagccaggcc gccactgcgc ggcgccccag gcgccatgcc tgccaggcca gcaccagcag
      721 caccagggtc agggccatgc cgctccagcg gtgcagcatg tgtaccagtg cgcctgcggc
      781 atggccaggc agcaggtcgc cggcgggcag cgcccacgga tcgagtgcct gccaggagcc
      841 ggcacgccaa tcgcaggccc cgagggcggg gcagctcagg cccgcctcgc cggcactgac
      901 catgccgcca agcgccacct gcgcccaggc cagggccgtg gccacggcaa gccatgcgga
      961 tggcaggggc cttgatcggg ctgcgtcggc atgccccgcc atcaaggcca ggcgcacgct
     1021 gagcgcgcac atggcaaagc ccgccagcag gttgcccagc gtgaccgcgg gcaggcgcga
     1081 cggcccggcc aggacgccca gcagtgccag aaacagcacc agccccagca agcccagcgc
     1141 cagacgcgcc tcggcgcgca ttgccacgcc cgcagacagg gccagcccca ccatcgccag
     1201 caccagcagc agggtcgccg aggccaccac cctgtgaccc atgcgcgcca tggccacccc
     1261 cgcgccagcc tctggcagcg tcgcgccgac ctgcacggcg gcgatgccct ggcactgtgg
     1321 ccagggttca cagccctgtt ccgagcggga ctgacggatg aaggcactca ggctggcgac
     1381 caccagcatc agggccgcgc acagccaggc caggcgttgc aataacaggc gccgacgggt
     1441 tggggaagca aaagagagca tgaaacagac agaaggaaag ccccaaaggc gcgcaggttg
     1501 acgcaagtcc agcgccaacg ccgtcaagac cgtggattat tcaaatcaag ggtttgcccc
     1561 attccatatc aagaacctga catgccgggc caggcgattg tggcatcagg gctgcgggtg
     1621 ggc
//
LOCUS       DAOMTQ010000307         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_24111_length_1617_cov_28.132522, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000307 DAOMTQ010000000
VERSION     DAOMTQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24111_length_1617_cov_28.132522"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1617)
                     /locus_tag="PK518_16145"
     CDS             complement(<1..>1617)
                     /locus_tag="PK518_16145"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HRM50487.1"
                     /translation="AARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLS
                     LVVEREEKIRKFVSRDYWEIHAGFHAEAGEYLGKWFDPQWKKSDDPEARADRLWSQRD
                     AETIAHAVRGKAATVTEESKPTTQASPLLFDLTSLQREANGKFGFSAKTTLALAQALY
                     ERHKALTYPRTDSRALPEDYLPVAKNTFGMLATSGMRHLAPYAQQALDDGYVRPSKRI
                     FDNAKVSDHFAIIPTTQAPHGLSEAEQKLYDLVVRRFMAVFFPSAEYQVTTRISQVVG
                     HSFKTEGKVLVRPGWLAIYGKEAANEVEDGKAGDKGQPLVPVKPGEMTHTEFADVKGL
                     KTKPPARYSEATLLGAMESAGKQIDDEELRSAMQEKGLGTPATRAAIIEGLLTEKYML
                     REGRELIPTAKAFQLMTLLRGLDVQELCRAELTGEWEYKLAQMERGQLSREAFMQQIQ
                     AMTEKLVKKAKEYDRDTIPGDYATLAAPCPNCGGVVKENYRRYACTGAAGSGEGCGFS
                     FGKSPAGRTFEVAEAEQLLRERRIGPLEGFR"
BASE COUNT          203 a          528 c          577 g          309 t
ORIGIN      
        1 cgaaagccct ccagcgggcc tatgcggcgc tcgcgcaaga gttgctcggc ctcggcgacc
       61 tcaaaggtgc gcccggcagg cgacttgccg aacgagaagc cgcagccctc gccgctgccc
      121 gccgccccgg tgcaggcgta gcggcggtag ttttccttca ccacgccgcc gcagttgggg
      181 cagggcgcgg ccagggtggc gtagtcgccg gggatggtgt cgcggtcgta ttccttggcc
      241 ttcttgacca gcttctcggt catggcctgg atctgctgca taaaggcctc gcgcgagagc
      301 tggccgcgct ccatctgcgc gagcttgtat tcccactcgc cggtgagctc ggcgcggcac
      361 agctcctgca cgtcgaggcc gcgcagcagc gtcatcagct ggaaggcctt ggccgtgggg
      421 atgagctcgc ggccttcgcg cagcatgtat ttctcggtca gcaggccttc gatgatggcg
      481 gcgcgcgtgg ccggcgtgcc caggcctttc tcttgcatgg cgctgcgcag ttcctcgtcg
      541 tcgatctgct tgccggcgct ctccatggcg cccagcagcg tggcttcgct gtagcgcgcg
      601 gggggtttgg tcttcaggcc cttcacgtcg gcaaactcgg tgtgcgtcat ctcgccgggc
      661 ttgacgggca ccaggggctg gcccttgtcg ccggccttgc cgtcttccac ctcgttggcg
      721 gcctccttgc cgtagatggc cagccagccg ggcctgacca gcaccttgcc ctcggtcttg
      781 aacgagtggc ccacgacctg gctgatgcgc gtcgtgacct ggtattcggc gctggggaag
      841 aacaccgcca tgaagcggcg caccaccagg tcgtagagct tctgctcggc ctcggacagg
      901 ccatgcggcg cctgcgtggt ggggatgatg gcgaagtggt ccgacacctt ggcgttgtcg
      961 aagatccgct tggatggccg cacatagccg tcgtccagcg cctgctgggc gtagggcgcc
     1021 aggtggcgca tgccgctggt ggccagcatg ccgaaggtgt tcttggcgac gggcaggtag
     1081 tcctcgggca gggcgcgcga gtcggtacgc gggtaggtca gggccttgtg gcgctcgtac
     1141 agggcctggg ccagggccag cgtggtcttg gcgctgaagc cgaacttgcc gttggcctcg
     1201 cgctgcaggc tggtcaggtc gaacagcagc gggctggcct gggtggtggg cttgctttcc
     1261 tccgtcaccg tggcggcctt gccacgcacg gcgtgggcta ttgtctcggc atcgcgctgc
     1321 gaccacaggc ggtcggcgcg ggcctcgggg tcgtcgcttt tcttccattg ggggtcgaac
     1381 cacttgccca ggtattcgcc ggcctcggcg tgaaagccgg cgtggatctc ccagtagtcg
     1441 cggctgacga acttgcggat cttttcctcg cgctccacca ccagcgacag cgtgggcgtc
     1501 tgcacccggc ccacggtggt caggaagaag ccgccatcgc gcgagttgaa ggccgtcatg
     1561 gcgcgcgtgc cgttgatgcc gaccagccag tcggcctcgg agcggctgcg cgcggcg
//
LOCUS       DAOMTQ010000308         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_24390_length_1605_cov_26.434194, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000308 DAOMTQ010000000
VERSION     DAOMTQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24390_length_1605_cov_26.434194"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PK518_16150"
     CDS             <1..702
                     /locus_tag="PK518_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRM50488.1"
                     /translation="GVLDADIYGPSQPMMLGISGRPESFDGKNMEPLVNHGVQVMSIG
                     FLVEQDQAMIWRGPMATQALEQLLRQTNWKDLDYLIVDMPPGTGDIQLTLSQRVPITG
                     AVIVTTPQDIALIDARKGIKMFEKVNVPILGLVENMAQHVCSNCGHVEHIFGADGGKK
                     MAAEYGIDYLGALPLARQIREQADSGRPTVVADPDGEVAQIYKHVAREVAVKIALKSK
                     DFSSKFPTISVSKST"
     gene            740..>1605
                     /locus_tag="PK518_16155"
     CDS             740..>1605
                     /locus_tag="PK518_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM50489.1"
                     /translation="MSQSSGSAGARQRAAGTSSFLSLGWRTLWRDLRAGELRLLMVAV
                     TLAVAALTSVGFFADRLQGGLARDAVQLLGGDAVVASDNPTPAAFVQQARTLGLASVT
                     TLSFPTMGRASDAQGGASRLVALKSVEAGYPLRGHVQVTDDPQRPGSPAQDIPAPGEV
                     WVDAPLLDSLGLQLGDYLLLGDTRLRATRIITLEPDRGAGFMSFAPRVMVNAADLSAT
                     GLVQPASRITYRFAVAGNPAAVQRFSDWAARAAEQPGMHGVRVETLDSGRPEMRQTLD
                     RAQKFLSLVALLA"
BASE COUNT          246 a          543 c          567 g          249 t
ORIGIN      
        1 ggcgtgctgg acgccgacat ctacggcccc agccagccca tgatgctggg catcagcggc
       61 cgccccgaga gctttgacgg caagaacatg gagccgctgg tgaaccatgg cgtgcaggtc
      121 atgtcgattg gcttcctggt ggagcaggac caggccatga tctggcgcgg ccccatggcc
      181 acgcaggcgc tggagcagct cttgcgccag accaactgga aggatctgga ctatctgatc
      241 gtggacatgc cgccgggcac cggcgacatc cagctgacgc tgtcgcagcg cgtgcccatc
      301 accggcgccg tcatcgtcac cacgccgcag gacattgcgc tcatcgatgc cagaaagggc
      361 atcaagatgt tcgagaaggt gaacgtgccc atcctcgggc tggtcgagaa catggcccag
      421 catgtgtgca gcaactgcgg ccatgtggag catatctttg gcgccgatgg cggcaagaag
      481 atggcggccg agtacggcat cgactacctg ggcgcgctgc cgctggcgcg gcagatccgc
      541 gagcaggccg acagcggccg gcccaccgtg gtggccgacc ccgacggcga ggtggcacag
      601 atctacaagc atgtggcgcg cgaggtggcg gtgaagatcg ccctcaagtc caaggatttt
      661 tcgtccaagt tccccaccat ctcggtgagc aagagcacct gacggcaccc cggtgcaagc
      721 cggtggcatg atgccggcca tgtctcaatc ttcaggctcg gcaggcgcgc ggcagcgcgc
      781 tgccggtaca tcctcttttc tttccctggg ctggcgcacg ctgtggcgcg acctgcgcgc
      841 gggcgagctg cggctgttga tggtcgccgt cacgctggcc gtggcggcgc tgacctcggt
      901 gggctttttt gccgatcgcc tgcagggcgg gctggcgcgc gacgccgtgc agctgctggg
      961 cggcgacgcc gtcgtggcca gcgacaaccc cacgccggcg gcgtttgtgc agcaggcgcg
     1021 cacgctgggc ctggcgagcg tcaccacgct gagctttccc accatgggcc gcgccagcga
     1081 cgcccagggc ggcgccagcc gcctggtggc gctcaagagc gtggaggccg gctacccgct
     1141 gcgcggccat gtgcaggtca ccgacgaccc gcagcgcccc ggcagccctg cgcaggacat
     1201 accggcgccg ggcgaggtct gggtggacgc gcccttgctc gactcgctgg ggctgcagct
     1261 gggcgactac ctgctgctgg gtgatacgcg gctgcgcgcc acgcgcatca tcacgctgga
     1321 gcctgaccgc ggcgccggct tcatgagctt tgccccgcgc gtcatggtga acgcggccga
     1381 cctgagcgcc accggcctgg tgcagccggc cagccgcatc acctaccgct ttgccgtggc
     1441 cggcaacccc gcggccgtgc agcgcttttc tgactgggcc gcgcgggcgg cggagcagcc
     1501 cggcatgcat ggcgtgcgcg tggagacgct ggacagcggc cgccccgaga tgcgccagac
     1561 gctggatcgg gcgcaaaaat tcttgagcct ggtggcgctg ctggc
//
LOCUS       DAOMTQ010000309         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_24540_length_1598_cov_32.000648, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000309 DAOMTQ010000000
VERSION     DAOMTQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24540_length_1598_cov_32.000648"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK518_16160"
     CDS             <1..366
                     /locus_tag="PK518_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HRM50490.1"
                     /translation="GGAGGSGGSALGGALGGGADLSALSALGLTSSGTASNAAGVITY
                     CMKNNYLNADKAAQVKNQLLGKMGLGQKEEPKDEGYLSGLSGMVTGSGGKSFSLDKVK
                     GDLKEKACDYVLDNAKSLI"
     gene            383..1027
                     /locus_tag="PK518_16165"
     CDS             383..1027
                     /locus_tag="PK518_16165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM50491.1"
                     /translation="MQTTRIIAIRHGETAWNVDTRIQGHLDIPLNDTGLWQARQVGQA
                     LADEPVAAIYSSDLQRAYATAQAVAQATGAPLTPERGLRERGFGDFQGRTFQQIERES
                     PEQALRWRKRDPHFVPDGGGESLVTLRERIACTVDEIAARHCGQQVVLVAHGGVMDVL
                     YRLATRLDLQAPRTWQLTNAAINRLLWTGGNGLTLVGWADTQHLEQHSRDEIHS"
     gene            complement(1063..>1598)
                     /locus_tag="PK518_16170"
     CDS             complement(1063..>1598)
                     /locus_tag="PK518_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM50492.1"
                     /translation="CIAKGKARTPYEFGVKVSIATTLKEGPVVGMRSMPGNPYDGHTL
                     AETLEQVGILTGADKPPKTVIVDKGYQGVEVPGVRILRSGQKRGITKTLRAMIKRRSA
                     VEPTIGHMKMDGRLARNPLKGALGDAIHAVMCGAGHNLRLILAALRLFCARLGVAVQE
                     IIGLLADRLRFAGAVFA"
BASE COUNT          280 a          563 c          516 g          239 t
ORIGIN      
        1 gggggcgcgg gcggttccgg cggctcggcg ctgggcggcg cgctcggcgg cggcgccgac
       61 ctgtcggccc tgtcggcctt gggtctgacc agctcgggca cggccagcaa cgcggccggc
      121 gtgatcacct actgcatgaa gaacaactac ctgaacgccg acaaggccgc acaggtcaag
      181 aaccagctgc tgggcaagat gggcctgggc cagaaggaag aacccaagga cgagggctac
      241 ctgagcggcc tgtcgggcat ggtcacgggc tcgggcggca agagcttcag cctggacaag
      301 gtcaagggcg acctgaaaga aaaggcctgc gactacgtgc tcgacaacgc caagtcgctg
      361 atctgaacac ccgagagcca ccatgcagac cacccgcatc atcgccatac gccacggtga
      421 gaccgcctgg aacgtggaca cgcgcatcca gggccatctg gacattccgc tgaacgacac
      481 cggcctgtgg caggcgcgcc aggtcggcca ggcgctggcc gacgagcccg tggccgccat
      541 ctacagcagc gacctgcagc gcgcctacgc cacggcccag gccgtggccc aggccacggg
      601 cgcgcccctc acgcccgaac gcggcctgcg cgagcgcggc tttggcgact tccagggccg
      661 caccttccag cagatcgagc gcgaatcgcc cgaacaggcg ctgcgctggc gcaagcgcga
      721 cccacatttc gtgcccgatg gcggcggcga gtccctcgtc acgctgcgcg agcgcatcgc
      781 ctgcaccgtc gatgagatcg ccgcgcgcca ctgtggccag caggtcgtgc tggtggccca
      841 tggcggcgtg atggacgtgc tgtaccgcct ggccacgcgc ctggacctgc aggcgccgcg
      901 cacctggcag ctgaccaacg ccgccatcaa ccgcctgctg tggacgggcg gcaacggcct
      961 gacgctggtg ggctgggcgg acacccagca cctggaacag cacagccgcg atgagattca
     1021 ctcctgatgc gctggtctag tccgccctga acaattcagt tttcaagcaa agaccgcccc
     1081 cgcaaagcgc agccggtcgg ctaatagccc gataatttcc tgaaccgcca cccccaatcg
     1141 ggcgcaaaaa agccgcagcg cggccaggat caggcgcagg ttgtggcctg cgccgcacat
     1201 cacggcatgg atggcatcgc ccagggcgcc cttgagcggg ttgcgcgcca atcggccatc
     1261 catcttcatg tgcccgatgg tgggctccac ggcgctgcgc cgcttgatca tcgccctgag
     1321 ggtcttggtg atgccccttt tctgccccga gcgcaaaatg cgcacccctg gcacctcaac
     1381 gccctggtag cccttgtcca cgatgaccgt cttcgggggc ttgtccgctc cggtgaggat
     1441 gcccacctgc tccagcgtct cggccagggt atgaccgtca tacggattgc ccggcatgct
     1501 gcgcatgccc accaccgggc cttccttcaa ggtggtggcg atgctgacct tgacgccaaa
     1561 ctcgtagggc gtcctggcct tgcccttggc gatgcatt
//
LOCUS       DAOMTQ010000310         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_24575_length_1597_cov_17.166018, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000310 DAOMTQ010000000
VERSION     DAOMTQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24575_length_1597_cov_17.166018"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PK518_16175"
     CDS             complement(<1..907)
                     /locus_tag="PK518_16175"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HRM50493.1"
                     /translation="MTAPLPTLAEIEAAAQLIYRQFQPTPQYRWGLLAERLGAECWLK
                     HENHGPVGAFKIRGGLSYFAQLAQDGRLPREVISATRGNHGQSIGWAARLHGVACTIC
                     VPHGNSVEKNAAMRALGVNLVEHGEDFQAAREHALQLAGQRGAHMVPSYHPALVRGVA
                     SYWWEFLRAVPRMDWAYVPIGLGSGACAAVAAKRALGHGVRLVGVVSSGARTYAESFA
                     AGRVVESPVSTQLADGMAVRLADPEALAILRGGLDHLVQVTDDEVAAAMRALFTDTHN
                     LAEGAGAAALAGALQEKDMLHGQCVG"
     gene            complement(911..>1597)
                     /locus_tag="PK518_16180"
     CDS             complement(911..>1597)
                     /locus_tag="PK518_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM50494.1"
                     /translation="FMYLLPNFQNPTGRTMSEERRAALSAKAAELGIPLVEDNPYGDL
                     WFDQPPPAPLTARNPEGCIYLGSFSKVLAPGLRLGYVVAPKAIYPKLLQAKQAADLHT
                     PSFNQRIVAEVLKDGFLARHVPTIRALYKHQRDAMLAALEREMAGLGVQWNSPAGGMF
                     LWVRLPEGMNAIELLPKAVERNVAFVPGAAFYADHGDPRTLRLSFVTASAAQIDTGIA
                     ALAATIRQAL"
BASE COUNT          224 a          547 c          593 g          233 t
ORIGIN      
        1 ggcccacgca ttggccgtgc agcatgtcct tttcctgcag tgcgccggcc agggcggcgg
       61 cgcccgcgcc ctcggccagg ttgtgcgtgt cggtgaacag ggcgcgcatg gcggcggcca
      121 cctcatcgtc cgtgacctgc accaggtgat cgagcccgcc cctgaggatg gccagcgcct
      181 ccgggtcggc caggcgcacg gccatgccgt cggccagctg ggtgctgacg ggcgactcga
      241 ccacgcggcc ggcggcaaac gattcggcat aggtgcgcgc gccgctgctg accacgccca
      301 ccaggcgcac gccatggccc agggcgcgct tggccgccac ggcggcgcag gcgcccgagc
      361 ccaggccgat cggcacatag gcccagtcca tgcgcggcac ggcgcgcaga aattcccacc
      421 agtagctggc tacgccgcgc accagggccg gatggtagct gggcaccatg tgggcgccgc
      481 gctgcccggc cagctggagc gcatgctcgc gcgcggcctg gaagtcctcg ccatgctcca
      541 ccaggttgac gcccagcgcg cgcatggcgg cgttcttttc caccgagttg ccatgcggca
      601 cgcagatggt gcaggccact ccatgcaggc gcgcggccca gccaatgctc tggccatggt
      661 tgccgcgcgt ggcgctgatg acctcgcgcg gcagccggcc gtcttgcgcc agctgggcaa
      721 agtagctcag gccgccgcgg atcttgaacg cgcccacggg gccgtggttc tcgtgcttga
      781 gccagcactc ggcgcccagg cgctcggcca gcaggcccca gcggtactgg ggcgtgggct
      841 ggaactgccg gtagatgagc tgcgccgcgg cctcgatctc ggccagcgtg ggcagcggcg
      901 cggtcatggt ctagagcgcc tggcggatgg tggcggccag ggccgcaatg ccggtgtcga
      961 tctgcgcggc gctggccgtg acgaaggaca ggcgcagcgt gcgcgggtcg ccatggtcgg
     1021 cataaaaggc ggcgccgggc acgaaggcca cgttgcgctc cacggccttt ggcaaaagct
     1081 cgatggcgtt catgccctcg ggcaggcgca cccacagaaa catgccgccg gcggggctgt
     1141 tccattgcac gcccaggccg gccatctcgc gctccagcgc ggccagcatg gcgtcgcgct
     1201 ggtgcttgta gagcgcgcga atggtgggca catggcgcgc caggaagccg tccttgagca
     1261 cctcggcgac gatgcgctgg ttgaagctgg gcgtgtgcag gtcggccgcc tgcttggcct
     1321 gcagcagctt ggggtagatg gccttgggcg ccaccacata ccccaggcgc aggcccgggg
     1381 ccagcacctt ggagaagctg cccagataga tgcagccctc ggggttgcgc gccgtcagcg
     1441 gtgcgggcgg cggctggtcg aaccacaggt cgccgtaggg gttgtcttcg accagcggga
     1501 tgcccagctc ggcggccttg gccgacagcg cggcgcggcg ctcctcgctc atggtgcggc
     1561 cggtggggtt ctggaagttg ggcagcaggt acatgaa
//
LOCUS       DAOMTQ010000311         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_24739_length_1590_cov_31.117915, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000311 DAOMTQ010000000
VERSION     DAOMTQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24739_length_1590_cov_31.117915"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PK518_16185"
     CDS             complement(<1..1185)
                     /locus_tag="PK518_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM50495.1"
                     /translation="MKKSFTTVLAMLACALGAAQAQDVYTIGEINSYKAQPAFLEPYK
                     KGMELALAQINITGGIGGKKLVLVTRDDNGNPADAVRAAEELIAREKVDVLMGSFLSH
                     VGLALTDFAQQKKRFFLAAEPLTDKIVWEQGNRYTFRLRPSTYMQVAMLVPEAAAMKK
                     KRWAIVYPNYEYGQSAVATFKKLLTAAQPDVEFVAEQATPLGKVDAGSVVQALADARP
                     DAIFNVLFAADLAKFVREGNTRGLFQGKGVVSLLTGEPEYLDTLQGETPLGWVVTGYP
                     WYAIFTPDHDAFATAYRKRFKDYPRAGSVVGYNAIQSIAAGLRKAGSADTEALIAAFR
                     GLEVRTPFGPITYRAQDHQSTMGAYVGKTGLYGGKGVMVNFKYMDGAKFQPSDAEVKK
                     LRP"
     gene            1388..>1590
                     /locus_tag="PK518_16190"
     CDS             1388..>1590
                     /locus_tag="PK518_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRM50496.1"
                     /translation="MSAFLEERVLSVHHWTDRLFSFTTTRDPALRFSNGHFTMIGLKV
                     NDKPLLRAYSIVSANYEEHLEFLS"
BASE COUNT          260 a          542 c          499 g          289 t
ORIGIN      
        1 cggccgcagc ttcttcacct cggcgtcgga cggctggaac ttggcgccat ccatgtactt
       61 gaagttcacc atcaccccct tgccgccgta cagcccggtc ttgcccacat aggcgcccat
      121 ggtggactgg tggtcctggg cgcggtaggt gatggggccg aacggcgtgc gcacctccag
      181 gccgcggaag gcggcgatga gcgcctcggt gtcggcgctg ccggccttgc gcagccccgc
      241 ggcgatggac tggatagcgt tgtagcccac caccgagccg gcgcgcggat agtccttgaa
      301 gcgcttgcga taggcggtgg caaaggcgtc gtggtcgggt gtgaagatgg cgtaccaggg
      361 gtagccggtg accacccagc ccagcggcgt ctcgccctgc agcgtgtcca ggtactcggg
      421 ctcgcccgtg agcaggctga ccacgccctt gccctggaac aggccgcgcg tattgccctc
      481 gcggacaaac ttggcgagat cggccgcgaa cagcacgttg aagatggcgt ccggcctggc
      541 atcagccagc gcctgcacca cgctgccggc gtccaccttg cccagcggtg tggcctgctc
      601 ggcgacaaac tccacatccg gctgcgctgc ggtaagcagc ttcttgaacg tggccacggc
      661 cgactggccg tactcgtaat tggggtagac gatggcccag cgctttttct tcatggccgc
      721 ggcctcgggc accagcatgg ccacctgcat gtaggtcgag gggcgcaggc ggaaggtgta
      781 gcggttgccc tgctcccaga cgatcttgtc ggtcagcggc tcggcggcca ggaagaagcg
      841 cttcttctgc tgcgcgaagt cggtcagcgc cagccccaca tgcgacagaa agctgcccat
      901 gagcacgtcc accttctcgc gcgcgatcag ctcctcggcc gcgcgcacgg cgtcggccgg
      961 gttgccgttg tcgtcgcgcg tgaccagcac cagcttcttg ccgccaatgc cgccggtgat
     1021 gttgatctgc gccagcgcca actccatgcc cttcttgtag ggctcgagga aggccggctg
     1081 ggccttgtag ctgttgatct cgccaatggt gtagacatcc tgcgcctggg ccgcgcccag
     1141 ggcgcaggcc agcatggcca ggacggtggt gaatgacttc ttcatgcgtg aatccttgct
     1201 gcagcgcaac agccgcggcc cgcaactgta ccgccgccgc cgccttgccc cacaatatgc
     1261 gggtttgccc gcatgacggg cgggcggttc ggcgcaattc ggcttatgcc tgacacgaat
     1321 aaaaaaaccg cgacactagc cgcaaagccc agcctccccc acttttcatc tttcttcctg
     1381 tccatccatg agcgcattcc tcgaagagcg tgtcctgtcg gtgcaccact ggaccgatcg
     1441 cctattcagc ttcaccacca cgcgcgaccc ggcgctgcgg ttctccaacg gccacttcac
     1501 catgatcggc ctgaaggtga acgacaagcc gctgctgcgc gcctacagca tcgtcagcgc
     1561 caactacgag gagcacctgg agttcctgtc
//
LOCUS       DAOMTQ010000312         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_25069_length_1577_cov_24.187254, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000312 DAOMTQ010000000
VERSION     DAOMTQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25069_length_1577_cov_24.187254"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK518_16195"
     CDS             complement(<1..300)
                     /locus_tag="PK518_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM50497.1"
                     /translation="MNRAALDRPVASVRRYAGEHQAHDHAHAQVLYALQGRMELELAG
                     RAAFVDAACGVVVPAGTRHGFLAGAGASILVIDAPPAAEVDRVRRFAVPPQARAEP"
     gene            343..>1577
                     /locus_tag="PK518_16200"
     CDS             343..>1577
                     /locus_tag="PK518_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM50498.1"
                     /translation="MSSHSAPAARRSWRQAWLVYLEPASLRMLALGFSAGLPLLLVLG
                     TLSFRLREAGVDRTTIGYLSWVGLAYAFKWVWSPLVDRLPLPLLTRRLGRRRGWLLLA
                     QGLVMAGLVGMALTDPRAELAPLVWCALLVAFGSATQDIALDAFRIESADAERQAALA
                     ATYQTGYRLAMIWAGAGVLWIAARAEVAGVDGYQAGAWAAAYLVMAASMLVGTVTVLL
                     SPEPAPRPLPPAKGLGDWLHGAVVAPFADFIGRYRWQAALILALIAVYRISDVVMGIM
                     ANPFYVDMGYSKDEVAAVTKVFGVVMTLAGAFIGGVLSMRLGVMRVLMLGAVLSALSN
                     LLFAWLGQRGHDVTGLVLVVSADNLAGGIASAAFIAYLSGLTNVQYSATQYALFSSMM
                     LLLPKWLAGFSGQFVDAHG"
BASE COUNT          171 a          562 c          579 g          265 t
ORIGIN      
        1 cggctcggcg cgcgcctgcg gcggcacggc aaagcggcgc acccggtcca cctcggccgc
       61 gggcggcgcg tcgatcacca ggatggatgc gccggcgccg gccagaaagc cgtgccgcgt
      121 gcccgccggc accaccacgc cgcaggccgc atcgacaaag gctgcgcggc cggccagctc
      181 cagctccatg cggccctgca gcgcgtacag cacctgggca tgggcgtggt cgtgcgcctg
      241 gtgctcgccg gcgtagcggc gcaccgaggc gacggggcga tcgagcgcgg cgcggttcat
      301 gggcgcgatg gtagccgcgc gcgggccgga taatcgcgcg ccatgtcgtc ccattccgct
      361 cccgccgcgc gccgctcctg gcgccaggcc tggctcgttt atctggagcc cgccagcctg
      421 cgcatgctgg cgctgggctt ttccgccggg ctgccgctct tgctggtgct gggcaccctg
      481 tccttcaggc tgcgcgaggc gggcgtggac cgcaccacca tcggctacct gagctgggtg
      541 ggcctggcct atgccttcaa gtgggtctgg tcgccgctgg tcgatcgtct accgctgccg
      601 ctgctcacgc gccgcctggg gcgcaggcgc ggctggctgc tgctggcgca gggcctggtg
      661 atggccggcc tggtcggcat ggcgctgacc gacccgcgcg ccgaactggc gcccctggtc
      721 tggtgcgcgc tgctggtggc ctttggctcg gccacgcagg acatcgcgct ggacgccttc
      781 cgcatcgaat cggccgacgc cgagcgccag gccgccctgg ccgccaccta ccagacgggc
      841 taccgcctgg ccatgatctg ggccggcgcg ggcgtgctgt ggatcgccgc gcgcgccgag
      901 gtcgcgggcg tcgatggcta ccaggccggc gcctgggccg cggcctatct ggtgatggcg
      961 gcctccatgc tggtcggcac cgtgacggtg ctgctctcgc ccgagcccgc gccgcgcccg
     1021 ctgcccccgg ccaagggcct gggcgactgg ctgcacggcg cggtggtggc gccgtttgcc
     1081 gacttcatcg ggcgctaccg ctggcaggcg gcgctgatcc tggcgctcat cgccgtctac
     1141 cgcatcagcg acgtggtcat gggcatcatg gccaacccct tctatgtcga tatgggctac
     1201 agcaaggacg aggtggccgc cgtcaccaag gtcttcggcg tggtcatgac gctggccggg
     1261 gcgtttatcg gcggcgtgct gtcgatgcgc ctgggcgtga tgcgcgtgct gatgctgggc
     1321 gccgtgctct cggcgctatc gaacctgctg ttcgcctggc tgggccagcg cgggcacgac
     1381 gtgaccggcc tggtgctggt ggtttcggcc gacaacctgg ccggcggcat cgcctcggcg
     1441 gccttcatcg cctacctgtc gggcctgacc aacgtgcagt actcggccac gcagtacgcg
     1501 ctgttcagct ccatgatgct gctgctgccg aaatggctgg cgggcttctc ggggcagttt
     1561 gtcgatgcgc atggcta
//
LOCUS       DAOMTQ010000313         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_25329_length_1566_cov_23.009265, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000313 DAOMTQ010000000
VERSION     DAOMTQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25329_length_1566_cov_23.009265"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..541
                     /locus_tag="PK518_16205"
     CDS             233..541
                     /locus_tag="PK518_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM50499.1"
                     /translation="MTRTAQAPANSSRVASPPGHWLLQDAKARFSELVRRVRSEGPQH
                     VTVHGRDEVVVIAAEEYRRLQGLQTGRALVEVLQATPYPEIDLAIAPPTYAPVRDVSL
                     "
     gene            538..969
                     /locus_tag="PK518_16210"
     CDS             538..969
                     /locus_tag="PK518_16210"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRM50500.1"
                     /translation="MKGYLLDTNILSELRRPRPEARVVDFVSAQPLESLFVSAVTFAE
                     IRFGIELVAEVHKRAALHDWLQQQLRPMFESRVLPVSEDVMFKWRLLVETGRKAGRTF
                     PQPDLIIAATALHHGLTVVTRDVGGFDGTDVPLLNPWQQPA"
     gene            1064..1564
                     /locus_tag="PK518_16215"
     CDS             1064..1564
                     /locus_tag="PK518_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM50501.1"
                     /translation="MDTRKTLLIVYHSLTGGTRQMAEAARDGAGAEPAVAVRLLHAAQ
                     AGPEDMLAADGYLFATPENLAAISGQMKDFFDRCYYPVLDRINGRPYASLICAGSDGQ
                     NAARQMARIATGWRLKMVAEPLIVCTHAQTPEVILAPKQIGAGDLEQCRALGEALATG
                     LALGVF"
BASE COUNT          262 a          494 c          520 g          290 t
ORIGIN      
        1 gcggccgggg tgtttgaggt gctataagca ccggcggctg ccccggtcaa cagaacaccc
       61 cgcatgggcc gcaaggcttg tgtggggtgt ttgcttttgt tttgatagct tctggcgctt
      121 tatgggtgcg cgtagcaggc cgaaataccg ttgttttttg cgaacttgcg tggccccagc
      181 agaacgcggg attcgcttgc acactatgag gtcggatgat attctggtca gtatgactag
      241 aactgcacaa gcccctgcca attccagccg cgtagcctcg cctccgggcc actggctgct
      301 gcaggacgcc aaggcccgct tcagcgagct ggtgcggcgc gtgcgcagcg aagggccgca
      361 gcatgtgacg gtgcacgggc gcgatgaggt cgtggtcatc gcggccgagg aataccggcg
      421 cttgcagggg ctgcaaacgg gccgagcgct ggtagaggta ttgcaggcca cgccataccc
      481 cgaaatcgac ctggccatcg cgccgcccac ctacgcaccg gtgcgagacg tgtcgctgtg
      541 aagggctacc tgctcgacac caacatcctt tccgagctgc gccgcccgcg ccccgaggcc
      601 agggtggttg acttcgtgag tgcgcagccg ctcgaaagcc tgtttgtcag cgcagtcacg
      661 ttcgccgaaa tccgttttgg catcgagctg gtcgccgagg ttcacaagcg cgccgccctg
      721 cacgactggc tccagcagca actgcggccc atgtttgaga gccgggtgct gccggtcagc
      781 gaagatgtga tgttcaagtg gcgcctactg gtggaaaccg gccgcaaggc cgggcgcaca
      841 tttccccagc ccgacctgat cattgcggcc acggcgctgc accatggcct gacggtcgtc
      901 acccgcgatg tgggtggctt cgatggcacc gatgtgccgc tgctcaaccc gtggcagcag
      961 ccagcatgag ccgacaagta tcaaggccag attgcccgtg tcatgcacga cattggcgac
     1021 gcccaattgg atacgtcata gccctggggt ctatgcttgc cccatggaca cccgcaaaac
     1081 tctgttgatc gtctaccact ccctgaccgg cggcacgcgc cagatggccg aggcggcgcg
     1141 cgacggtgcc ggggccgagc cggcggtggc ggtgcgcctg ctgcatgccg cgcaggccgg
     1201 gcccgaggac atgctggcgg ccgatggcta cctgtttgcc acgcccgaga atctggccgc
     1261 catcagcggg cagatgaagg atttttttga ccgctgctac tacccggtgc tcgatcgcat
     1321 caatggccgt ccctatgcca gcctgatctg cgccggcagc gacggccaga acgcggcgcg
     1381 ccagatggcg cgcattgcca cgggctggcg cctgaagatg gtggccgagc cgctgatcgt
     1441 ttgcacccat gcccagacgc ccgaggtcat cctggcgccc aagcagattg gcgcgggtga
     1501 tctggagcaa tgccgcgccc tgggtgaggc gttggccacg ggtttggcct tgggcgtgtt
     1561 ttaggc
//
LOCUS       DAOMTQ010000314         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_25841_length_1547_cov_32.211126, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000314 DAOMTQ010000000
VERSION     DAOMTQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25841_length_1547_cov_32.211126"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="PK518_16220"
     CDS             <1..1437
                     /locus_tag="PK518_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRM50502.1"
                     /translation="IYSIVKKMRGKSLNFDQVFDIRALRVIVPNVKDCYAALSWVHSQ
                     FSPIEAEFDDYIAKPKPNGYQSLHTVVRDEAGRAIEIQIRTQAMHDHAEHGVAAHWAY
                     KEAGTKGYAGVSAASEYDAKIAVLRQLLAWERDLVGSASRSSLFEDRIYVLTPDAAVV
                     ELPQGATPVDFAYAVHTNVGHRCRGARVDGAMVPLNTPLQNGQTVEISTVKEGRPSRD
                     WLNPELGYLVSHRAKAKVRAWFNAQATAVTVLRGRELVEKLLQREGKTALKHDDLAEQ
                     LGFKTADALFEMVGKDELSLRSIETLLRPPEPVAPTPDMLVRKARASDGGAKGGVLVV
                     GVDSLMTQLAKCCKPAPPDDIRGFVTRGKGVSVHRSDCRNFHEMFARNAERVIDVQWG
                     KQQRQAAGAAVYPVDVAVEASDRQGLLRDISEVFAREKTNVIGVQTQSVKGTAWMTFT
                     VEVSDAGRLNKVLGMVANVQGVRSARRR"
BASE COUNT          282 a          499 c          519 g          247 t
ORIGIN      
        1 atctacagca tcgtcaagaa gatgcgcggc aagtcgctca acttcgacca ggtgttcgac
       61 atacgcgcgc tgcgcgtgat cgtgcccaac gtcaaggact gttacgccgc cctgagctgg
      121 gtgcacagcc agttcagccc catagaggcc gagtttgacg actacatcgc caaacccaag
      181 cccaacggct accagtctct gcacaccgtg gtgcgtgacg aggcggggcg cgccatcgag
      241 atccagatcc gcacccaggc catgcacgac catgccgagc atggcgtggc cgcgcactgg
      301 gcctacaagg aggcgggcac caagggctat gccggcgtgt ccgcggccag cgagtacgac
      361 gcaaagattg ccgtgctgcg ccagctgctg gcctgggagc gcgacctggt cggctccgcc
      421 agccgcagca gcctgttcga agaccgcatc tatgtgctga cccccgacgc cgccgtggtc
      481 gagctgccgc agggcgccac gcccgtggac tttgcctatg ccgtgcacac caatgtcggc
      541 caccgctgcc gcggtgcgcg cgtcgacggc gccatggtgc cgctgaacac gccgctgcag
      601 aacggacaga ctgtggagat cagcaccgtg aaggagggcc ggccctcacg cgactggctc
      661 aacccggagc tgggctacct cgtcagccac cgcgccaagg ccaaggtgcg cgcctggttc
      721 aacgcccagg ccaccgcggt cacggttctg cgcgggcgcg aactggtgga aaagctgttg
      781 cagcgcgagg gcaagacggc cctcaagcac gacgacctgg ccgaacaatt gggcttcaag
      841 acggccgacg cgctgttcga gatggtgggc aaggacgagc tctcgctgcg ctccatcgaa
      901 accctgttgc gcccgcccga gcccgttgcg ccaacgcccg acatgctggt gcgcaaggcg
      961 cgcgcgagcg acggcggcgc caagggcggg gtgctggtgg tgggcgtgga ttcgctcatg
     1021 acgcagctgg ccaaatgctg caagcccgcg ccaccggacg atatccgcgg ctttgtcacg
     1081 cgcggcaagg gcgtcagcgt gcaccgcagc gactgccgca attttcacga aatgtttgca
     1141 cgcaacgccg agcgcgtgat cgacgtgcag tggggcaaac agcagcgcca ggccgcgggc
     1201 gctgcggtct atcccgtgga cgtggccgtg gaggcctcgg atcggcaggg gctgctgcgt
     1261 gacatctcgg aggtgttcgc gcgcgagaaa accaacgtca tcggcgtgca gacccaatcc
     1321 gtgaagggca cggcctggat gaccttcacc gtggaggtgt ccgatgccgg ccggctgaac
     1381 aaggtgctgg gcatggttgc caacgtgcaa ggcgtgcgtt cggcacgcag gcgttgagtc
     1441 gatttttttt gggccgtttt ttgctgctat actcccagct tctcgaacaa acacaggcgc
     1501 gtagctcagc tggttagagc accaccttga catggtgggg gtcgttg
//
LOCUS       DAOMTQ010000315         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Alicycliphilus sp. isolate AMR_MDS_5752
            NODE_26331_length_1528_cov_22.243720, whole genome shotgun
            sequence.
ACCESSION   DAOMTQ010000315 DAOMTQ010000000
VERSION     DAOMTQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867973
            Sequence Read Archive: SRR1616983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alicycliphilus sp. (wastewater metagenome)
  ORGANISM  Alicycliphilus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Alicycliphilus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.153
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:31:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,246
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,186
            CDSs (with protein)               :: 3,186
            Genes (RNA)                       :: 27
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 12 of 33
            Pseudo Genes (incomplete)         :: 23 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 5 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Alicycliphilus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26331_length_1528_cov_22.243720"
                     /isolate="AMR_MDS_5752"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1872437"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK518_16225"
     CDS             complement(<1..597)
                     /locus_tag="PK518_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRM50503.1"
                     /translation="MLALAVAMGVGRFAFTPLLPMMLHDGVVTLTEGSWLATANYVGY
                     LVGALACMALPWLLPRLYERWHPARLARAGLVATVLLTLAMALPLPDSWPALRFAAGV
                     ASAFVLLNTAAWAMVRLTVLGRPAMGGLIFCGPGVGIALTGLATSLMVQAQWRAASGW
                     VVFGLLSVLLCLVVWPVVQGRAAKAQAGAAHAPAAAARA"
     gene            783..>1528
                     /locus_tag="PK518_16230"
     CDS             783..>1528
                     /locus_tag="PK518_16230"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRM50504.1"
                     /translation="MHHDLLARLGLSLPIIQGPMTGSDTPQLAAAVSAAGGLGFLGCG
                     MRSPAAMLEAAAAVRAATDKPFGMNLFVLDSPRPEAAEVATALARLAPMYARFGLQPE
                     PPARWCEDFAMQFEALIQARPAVASFTFGILSSAQVRRIKMDAGSYVIGTATTPAEAL
                     AWAEVGADAVVASGLEAGGHRGTFLGDWEGSQIGTLALVPACVDALSIPVIAAGGIMD
                     GRGIAAAQALGAQAVQMGTAFLACPESAITP"
BASE COUNT          224 a          577 c          531 g          196 t
ORIGIN      
        1 ggcgcgcgcc gcagccgctg gcgcatgcgc cgcgcccgcc tgcgccttgg ccgcacgccc
       61 ctgcaccacg ggccagacca ccaggcacag caacaccgac agcaggccaa acaccaccca
      121 gcccgatgcc gcgcgccact gcgcctgcac catcaggctg gtcgccaggc cggtgagcgc
      181 aatgcccacg cccgggccgc agaaaatcag cccgcccatg gccgggcgcc ccagtaccgt
      241 gaggcgcacc atggcccagg cggcggtatt cagcagcaca aaggcgctgg ccacgccggc
      301 ggcaaagcgc aaggcgggcc agctgtccgg cagtggcagg gccatggcca gggtcagcag
      361 caccgtggcc accaggccgg cgcgcgccag gcgcgccggg tgccagcgtt catacaggcg
      421 cggcagcagc cagggcaggg ccatgcaggc cagggcgccc accaggtagc ccacgtaatt
      481 ggcggtcgcc agccagctgc cctcggtcag cgtgaccacg ccgtcgtgca gcatcatggg
      541 cagcagcggc gtgaaggcaa aacgccccac gcccatggcc acggccagcg ccagcatgcc
      601 ggccagggcc acggcggcgg ggcggtgcga ccagtcgcgc gcggagggcg aggtcggcgg
      661 ggcgggagtg gatgcgtcca tggtgatgcg ctggcaatgg ggaagcaggg attattgcca
      721 tggccctgcg ggtatcctgc ggtcgctatc gtctatctgc ctctcttctt cccctgcacg
      781 ccatgcacca cgacctactt gcccgcttgg ggctatcgct tcccatcatc cagggcccca
      841 tgaccggctc cgacacgccg cagctggccg ccgccgtatc cgccgccggc gggctgggct
      901 ttctgggctg cggcatgcgc tcgcccgccg ccatgctgga ggcggcagcc gccgtgcgcg
      961 ccgccaccga caagcccttt ggcatgaacc tgttcgtgct ggacagcccc cggcccgagg
     1021 ccgccgaggt agccaccgcc ctggccaggc tggcacccat gtatgcgcgc tttggcctgc
     1081 agcccgagcc gcccgcgcgc tggtgcgaag acttcgccat gcagttcgag gcgctgatcc
     1141 aggcccgccc ggcggtcgcc agcttcacct tcggcattct gagcagcgcc caggtgcgcc
     1201 gcatcaagat ggatgccggc agctacgtca tcggcaccgc cacgacgccg gccgaggccc
     1261 tggcctgggc ggaggtgggc gccgatgccg tggtcgccag cggcctggag gccggcgggc
     1321 accgcggcac ctttctgggc gactgggagg gcagccagat cggcaccctg gcccttgtgc
     1381 cggcctgcgt ggatgccttg agcattcccg tcatcgccgc cggcggcatc atggacgggc
     1441 gcggcatcgc cgccgcccag gccctgggcg cacaggcggt gcagatgggc acggcctttc
     1501 tggcctgtcc cgagtcggcc atcactcc
//
